LOCUS       DASSMB010000001        34063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_380237, whole genome shotgun sequence.
ACCESSION   DASSMB010000001 DASSMB010000000
VERSION     DASSMB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 34063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34063
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380237"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2264)
                     /locus_tag="VFJ72_00005"
     CDS             complement(<1..2264)
                     /locus_tag="VFJ72_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1695..1697),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7477872.1"
                     /translation="MSIRDSIAAWPVLRQVRGKDYLGLGSSARSARSASLAPLTSGAD
                     GVVKSVCPYCAVGCGQRVFVKDGRITQIEGDPDSPISRGRLCPKGAASRSLVQSPARE
                     YKIKYRRPYGTGWEDLDLETAMGMISDRLIKTRDETWQDEDEDGRHLHRTLGFAHLGG
                     ATLDNEENYLIKKFFTAMGAVQIENQARIUHSSTVPSLGTSFGRGGATTFQQDLANAD
                     SILIMGSNMAENHPVGFQWVMEARERGAKIMHVDPRFTRTSAMSNLHVPIRAGTDIAF
                     LGGIINHILENEKYFRDYVVHYTNAPVIINEQFADTEDLDGLFSGWDPESGSYDVDTW
                     QYAGMEVSGSAGKREQGGVTGEQAHYAQGGQLEHGEPTEMDESLEHPRCVFQLLKKHY
                     SRYTPEMVERICGCPKEKFLEVAETLCENSGRERTSAFCYAVGWTQHSYGVQKIRAAS
                     ILQLLLGNMGRPGGGIMALRGHASIQGSTDIPTLYNLLPGYLPMPHVTQDVTLADYVA
                     KNRSETSYWANIEEYLVSLMRAYWGAAATAENDWGYGYLPRIDQDHSVYRTVLGMLDG
                     TVEGYIVAGENPAVGSANSRLHRLAFAKLKWLVVRDLVEIETATFWKAGPEIETGELR
                     TEAIPTEVFLMPAAAHTEKDGSFTNTQRLLQWHHQAIEPPGDCRSELWFYYHLGRRIQ
                     EKLAGSKEPRDEGIRKLRWDYPTHGQIEEPSAEAVLAEINGFTVDGAPLSTYEQLKKD
                     GSTSCGCWIYCGVYA"
     gene            complement(2572..3630)
                     /locus_tag="VFJ72_00010"
     CDS             complement(2572..3630)
                     /locus_tag="VFJ72_00010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7477873.1"
                     /translation="MELGLYTFADVGPEITPEQRLRNLIEEIELADQAGLDVFGVGEH
                     HRPDYAVSSPAVALAAAAARTRNIRLTSAVTVLSSDDPVRVFQDFATLDLISGGRAEI
                     MAGRGSFIESFPLFGYDLGDYDDLFAEKLDLLLKLRESERVTWSGRHRAPLEDAGVYP
                     RPVQDPLPVWIAVGGTPQSVVRAAALGLPLAIAIIGGQPENFAPLARLYREAAARSGH
                     DPSRLPISINSHTYVAETSRQAGDEYFPAYAAMMNRIGRERGWGPMGRRQFEMGRSPR
                     GALLVGSPEEVVEKVLYEHELFGNQRFMAQISVGAMPHEKVMRAIELFGAEVAPAVRK
                     EIERRTSASAEGSAAARS"
     gene            complement(3834..4220)
                     /locus_tag="VFJ72_00015"
     CDS             complement(3834..4220)
                     /locus_tag="VFJ72_00015"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HET7477874.1"
                     /translation="MELLLLIGRILFGGFFIMSGINHFMKVGMISGYAGSKNVPAPGL
                     AVMGTGVMLVLGGLSVLLGFLPVLGLLLLILFLIPTSFLMHNFWTISDPMQRAAEQVN
                     FLKNIALTGAALALMYGAADWPLSLS"
     gene            complement(4319..4927)
                     /gene="wrbA"
                     /locus_tag="VFJ72_00020"
     CDS             complement(4319..4927)
                     /gene="wrbA"
                     /locus_tag="VFJ72_00020"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HET7477875.1"
                     /translation="MSVKVAVIYYSATGNVYKLAQAIKEGAEEADAEVRLRKVHELAP
                     EEAVKSNQGWYDHALMTEDVPEASMDDLEWADAYIFGTPTRFGNVSAQLKQFLDQTGG
                     LWFQGKLADKVVSGFTSAQNAHGGQESTLLSMYNVFMHWGAILVTPGYTDPVLFESGG
                     NPYGVSSTDGGEGGPSEQELAAARYQGKRVAEKAALLAGVQV"
     gene            5007..5681
                     /locus_tag="VFJ72_00025"
     CDS             5007..5681
                     /locus_tag="VFJ72_00025"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7477876.1"
                     /translation="MSRVQEQMVQEIGKKYGEMEWSLDQAGRALPPGTERKDAARNRR
                     KIVETARRLFSERGVTQVSMEEISRAAGVGKGTLYRRFPNKGLLCQALMDEPTREFQE
                     ETLRILGDARRGPLEKLDLFLDGLVRFTDENLDLLYGGNEPLCGADRLARFEHPAFDW
                     RRWTVLGLLRAAAREGDLNETLDLEYLAAALLAPLDVDFFYFQRRVREMTTKRISAGL
                     RSLVPT"
     gene            5745..6707
                     /locus_tag="VFJ72_00030"
     CDS             5745..6707
                     /locus_tag="VFJ72_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET7477877.1"
                     /translation="MELGGLHHMTAVTTVAARNVEFYTRVLGMRLVKKTVNQDDTSAY
                     HLFYGDELGRAGTEVTFFDWPQLRVAPNHPGAGEVSTTELRVRGREALDYWAGRLEEF
                     EVAHHGIEERDGRLAISFTDPEGQRLRLADDSAAVVEGGRQWEKSDVPAEFGVRGLGA
                     FDLTVREAAPTAWVLTDILGFRESAEYEEDGARVRAFEVGPGGPGAVVRLFERPDAPW
                     AHLGRGGVHHVAFRAPDDEQHEAWRKRIRSAGIGVTPQIDRYYFRSIYFREPGGVLFE
                     IATDGPGFATDEDAAHLGERLSLPPFLEDRREEIEARLEPIMVH"
     gene            6766..7305
                     /locus_tag="VFJ72_00035"
     CDS             6766..7305
                     /locus_tag="VFJ72_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7477878.1"
                     /translation="MGNLSALLLRLVLGGLLAGHGAQKLFGSFNGPGLEGTSGFMEML
                     GLKPGKPWAVMAGLSEFGGGVLTVLGLLNPLGPIGVIGAMSMATAKAHWGKPIWVTEG
                     GAELPVTNIAAATALILNGPGKYSLDRALGLKLPSWLAPLGLVAVILTVLYAGIGSGA
                     QPPEANQEEAREDLAGPEE"
     gene            7356..7496
                     /locus_tag="VFJ72_00040"
                     /pseudo
     CDS             7356..7496
                     /locus_tag="VFJ72_00040"
                     /inference="COORDINATES: protein motif:HMM:NF023949.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
     gene            7548..7955
                     /locus_tag="VFJ72_00045"
     CDS             7548..7955
                     /locus_tag="VFJ72_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7477879.1"
                     /translation="MRAAVFRDGKILLIKEPEDGLWSLPGGWADVGETPSETTAREVY
                     EESGYRVRVVRLLAAYDRDRQGHTPLPYHVYKLCFLCEIVGHDSSVAVEADGIGFFAE
                     DDLPPLSASRVTEAQIQTFFHQHRHPEAPAGFD"
     gene            complement(7959..8900)
                     /locus_tag="VFJ72_00050"
     CDS             complement(7959..8900)
                     /locus_tag="VFJ72_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7477880.1"
                     /translation="MRAVVVDPNAPHRLAISDVEEPAPAPSEALVRVAAVSLNRGEVR
                     RATGADEPGARPGWDLAGTVERAAADGTGPAEGARVVGFLPAGAWAELAAVPANALAE
                     LPDGVSFEQASTLPIAGLTALYALEKGSALLGRRVLVTGASGGAGLFALQLARLAGAR
                     VVALVRREEHVRLVRDAGAHEVAVGEDGAAAREYGPYDLVLESVGGAVLGNALSMLAT
                     GGTCVSFGVSGGAECTFDVRSLFWTGGAKLYGFIIFHEVLAHPASEGLARLVRLIEEG
                     NLMPRIEVEEDWAEIGAVADRLMQRGFTGKAVLRVGG"
     gene            9006..10028
                     /gene="corA"
                     /locus_tag="VFJ72_00055"
     CDS             9006..10028
                     /gene="corA"
                     /locus_tag="VFJ72_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET7477881.1"
                     /translation="MQPAATKSLPENVDLMVLDASGGLREEVGLEELPGYLADKRALI
                     WCDIYSTQGGQHGPYGRLLSGVFGFDELTVEDCFTRSHLPKVDIYEDYLFVALFSFHL
                     SEKTRRVQTVEVDMYFGENFVVCVHPRPLRELERVRRRLLSGDEFVSSSPANVAHTVF
                     DAVVDEYLPIMNRLSALVDGIEDDLLGEDNDVPEDVLDHLFHLKHELTALRRLAVPLR
                     DVVSILMRPTIRLIPEESRMHYDDVRDHLVRVIDMIDTMRDYLTGSLDIYTTQQTQRI
                     NQSMQRLTAISLIFLPLTFLTGIYGMNFSYMPETESHYGFYVLITLCALVGLGMLYYL
                     HRKRML"
     gene            complement(10045..10761)
                     /locus_tag="VFJ72_00060"
     CDS             complement(10045..10761)
                     /locus_tag="VFJ72_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477882.1"
                     /translation="MKAFLCVDGRDWEGVVRGAARYLADEGTATLAHVVDERAPRGYE
                     MSLRGLLGRRRPGDAEERMVRASREAAEGFLGDARDLLQRLRPGNMIETRVLDGDPNK
                     ELMRAAGEAGAQTIFISRGTPGSRSRETVSGIVEGWNQNREGRFDGLVLGDGTEVRFP
                     PHRAEAVRSGISDGAEIEASGVWQGRRVLHAYSITDTRNGVPIEAHESPEAMPGERPL
                     GHTARFVVDHALCDVVILNL"
     gene            complement(10799..12082)
                     /locus_tag="VFJ72_00065"
     CDS             complement(10799..12082)
                     /locus_tag="VFJ72_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET7477883.1"
                     /translation="MSAVLAGVILAATLGLVLTRPWDIGEAWWAVLGGGLTLVLGLVS
                     LGQAWDILLETQDALVLLIGMMSLSAVAEKAGFFDWAASITARAGRERVLLLYGFVFL
                     VGTLVTVTLSLDATAIVLTPIVYGMVVRLRLSPMPFMFACVYTANTASLFLPISNLTN
                     LLAYDRFDLGFARFGLVMLLPGVLAILTNFAIFVFLFRNDLRGNYDLGKGARFEAENK
                     GLFRVAAAGIGGVLVAFFAAPLLGIPIGFVALAAGAIVVIVSRAAGWVTLREVVGSVS
                     WGIIFLVVGLFLVVQGVQNAGLSGLVESAFAAAAPGDGFFQILALTFGSAIGANIINN
                     VPMTVLALGTMESLVKGGTLGVSAVYAMVVGTSIGPNLTTVGSLATLIWLSIARGRGL
                     DITAKDYLKIGAIAAPAILLAAAVGLWISVALLGT"
     gene            complement(12170..12406)
                     /locus_tag="VFJ72_00070"
     CDS             complement(12170..12406)
                     /locus_tag="VFJ72_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477884.1"
                     /translation="MNISSHPSADDLRQAIRARGGAAVCRECGSEDLSLEQVEPMDAA
                     GTGYGTRRLKRADLVCENCGHVMGYELDKLRSQG"
     gene            complement(12494..13072)
                     /locus_tag="VFJ72_00075"
     CDS             complement(12494..13072)
                     /locus_tag="VFJ72_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477885.1"
                     /translation="MGDKVKGLMMQYIEEMEGRSGRRPTKPVTFSMRLSERDHAKLVW
                     LAKNLDIAKTPLAEELLKAAVDEAIEQYAGWASSDRPEDFVEEMERAIPPAPPQRAAR
                     SFGSRATSVSGTVARWKANHHGDTDGFYLKDGREIGFPPHRAAEVQDLFREGTAIEAV
                     GEWHGERFHAYTIADTESGTRVEAHKPPRPGP"
     gene            13358..14722
                     /locus_tag="VFJ72_00080"
     CDS             13358..14722
                     /locus_tag="VFJ72_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7477886.1"
                     /translation="MSKTGDSGERREQPGENRSRGKAGRERVRFRDRGRKGGVRDRLR
                     NRPHIRPKVYGYLTEEKESLRQGFAALFLSSTGELVAGLALASITETLDQLVGLAVLI
                     PAAIGMRGAIFGAMGSRISTAIQTGLFSFNVRRGVLAENVQAAAVLSLLSGIFLAFLA
                     RGFCGVLGVRTELSVFDYVVISTVGGLLAGLLLLGITIIVARLSVARGWDMDNIAAPM
                     ITAAGDILTLPSLVLATYLIGIPVFSSALAAALILCAAVIVTLAFRLGAANLRRILAE
                     SLPILALTGMVTILVGVALQQREEQFSTLLALSILLPAFLQEGGALGGILSSRLSSKV
                     HLGLLAPRGIPDLAAFRDFTLTYIFAAGVYVFIGGASHFLAVGLFSTEASPGFVAMLG
                     ISALAGLIATTAAVFAAYYGSTISYRLGLDPDTYGIPIITAAVDLLGFMSLIISLVVF
                     GLVG"
     gene            14727..15932
                     /locus_tag="VFJ72_00085"
     CDS             14727..15932
                     /locus_tag="VFJ72_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HET7477887.1"
                     /translation="MRDTRRGTQPRNLKDMLAEAKNTSELMVDLAYAAIFYNSEDFSE
                     EVLRLEERLNDLVFDMRTLAILAARSPTDAEQMAGILGVVEDIEKIGNAAIDIAKIVL
                     KRLGIPQELLHDIPEAEEIPSRVRIHAESALDGRSLEEVDLPTETGMRPIAIRSGDEW
                     NFDPEPEDILRARDVLFMQGPPEGVAEVRELAGAAPLEPAPSVEAGALSELERAVDIL
                     IEMKNLSEVAVGLAYSALLYYDAGLAREVVAIENEMDEMRYRLERWVLLAARHIEDSS
                     RLRGVLHLATASETIADCAMEMVWIVEKGEEVHPVLSAAVGESDEVVMKLTVSPGSPA
                     DGRSLEELEFETETGMYALAINRGGRWTYRPRDNYVLLGGDSVLVTGAPEGVEPFAEL
                     FGQELEETP"
     gene            16004..17179
                     /locus_tag="VFJ72_00090"
     CDS             16004..17179
                     /locus_tag="VFJ72_00090"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET7477888.1"
                     /translation="MYALLFLRFAGLPPDLKDNSNDLRIYRDTGEAVLRGEFPYRDFF
                     IEYPPGSVPAFVPPAFFSDSTSGYITLFANEMALLLVAALALVALAARKMWTGYSWSV
                     PALTFAASAVLLYPVAVTRYDAVVALTLAVATWCAVRGGRWPVILGYASLGFGAAAKL
                     VPALAALPLALIRRRSAVPGFFVFFAVVALFFGPAVLLAGGQFADSFAYHAQRGLQVE
                     SVGSSVLMQLGWIREIVFEFGAFEARGRGTEFASSISLPVTAALLGLTALVMYRKHRE
                     GGTDAGLFARHAAALILAFMLGSKVLSPQYMIWLLPLLPLAAPRLTGLGVSALFLAAC
                     WTTTQVFPVHYNDLLTLRSPGPQLLLARNLLLAFLWVLMLLLPTSAPTGERSDKAAS"
     gene            17176..19062
                     /locus_tag="VFJ72_00095"
     CDS             17176..19062
                     /locus_tag="VFJ72_00095"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET7477889.1"
                     /translation="MKRSAILLLILLLLPLLSVTLGLRGGTHLDEQQARQRAELAPKV
                     ANLVEHPSVQPSATYDSVSDTWLVLLTEEVSGSVVAKVTVDDDTGDVVRTEISPNATG
                     ISYPSLSEEDATKLATADIRVRDYLSDFGSYSTDTEYENGEWTIHYRIQGTGAVGGVP
                     DPEAGTKEIVRAGVDDDTWVVEYVWAGDQVGWQMARGEYGSYGKQANYWYVWGPMALL
                     FALAFVRADKMFSLRNLDILMLLGFLVSHGFWRAGDSYEAVLLWYPPLIYLLVRTLLM
                     GFGIGERVEKTANFPTPVLFILAALAGGFVLALNLDARVIDVGYAGVVGADRILDGTL
                     PYGHMPSDVGTGDTYGPLNYLLYVPFVLMFGFSGKWDYLPAAHATTAFAFTVGALALL
                     YAGYRLAGARGAAAMVFAWAVFPYTLYSTNNNTNDVIVAAASAVSLAFATSPLGRGAS
                     IAAGFAIKLYPLILAPLWMAQDRWKRSSIVDFALGGVGVCLLTFWILTLDGDIISALK
                     LFYQKTITFQGDRETPWTIFAQVPQIAFLKRLLTAAVIFLAVLVAIFPRKRTVRRLAA
                     LSAALVIAVQLVTNYWFYTYVVWFEPFVFLALFTATNEKTALDGETEPPPEASVPQNA
                     GTGG"
     gene            complement(19066..20355)
                     /locus_tag="VFJ72_00100"
     CDS             complement(19066..20355)
                     /locus_tag="VFJ72_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET7477890.1"
                     /translation="MDLFDSSGSESLARRAPLAERLRPKTLDEVVGQEHLTGENGPLR
                     VVAERGRIGSVVFWGPPGTGKTTLARVLANTVEDEFVPLSAVTSGVKDLRAALDAARD
                     RLKYEGRGTLLFVDEVHRFNKVQQDALLPALEEGLVDFIGATTENPSFEVTAPLLSRS
                     RVLRLRSLSEEDLGTLLERGLSELGARISEDAREYLLRLSGGDGRRMLNALEVAAAGT
                     KNVEVADVERAVGRRALRYGREEHYDVISAFIKSVRGGDPDAALHYLARMIEAGEDPV
                     FIARRLVILASEDIGNADPRGLPLATAAAQAVQMIGMPEGRIPLAQATTYLASAPKSN
                     AAYAGINAALADVRREVTPEVPMHIRNAPTGLMKEEGYGANYRYAHTDEPEGMNDRYL
                     PDELAGRIYYEPKVSGVEARIKERLDRWRKAREERER"
     gene            complement(20398..21822)
                     /locus_tag="VFJ72_00105"
     CDS             complement(20398..21822)
                     /locus_tag="VFJ72_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7477891.1"
                     /translation="MPSGPETDLCLLGAVDLARLIRSRETSAVEVMRAHLERIEQANP
                     AVNAIVTFLPERALEGARRADRALHRGEEAGPLHGLPVAHKDLADTAGIRTTYGSRIY
                     KDHVPERDNLTVERLKRAGAISVGKTNTPEFGAGSQTFNAVFGATRNPYDLSKTCGGS
                     SGGAAVALACGMLPIADGSDMGGSLRNPAAFCNVVGLRPSPGRVPSWPSMAPWSPLSV
                     EGPMGRSVEDVALMLSAIAGPDPRAPLSISEPGGGFDPLPERDFRGTRIAWSPDLGGL
                     PVERQVREVFETSGKMMEEIGCGVDEAAPDFSGADEVFKVLRAWQFELAYGGLLDSHR
                     DLLKDTVVWNIEEGRRLTGPRIGAAERQRAEILERVRLFMERYEFLVLPTTQVAPFGV
                     RTPYVEEIDGEPMETYIDWMKSCYYITVTGLPAVSVPGGFTPEGLPVGVQIVGRWRDE
                     IGVLQLARAFERATRFGERRPEAL"
     gene            21928..22272
                     /locus_tag="VFJ72_00110"
     CDS             21928..22272
                     /locus_tag="VFJ72_00110"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET7477892.1"
                     /translation="MDEARHEVFELADRISDRLAEIAGVEAVALGGSWARGEAHPDSD
                     VDLGIYYRSGSPPRIEELRRLARELDDRHPDDSLTDFGGWGPGSTTVDGSRSKAATWT
                     GSTATSTSSSAP"
     gene            22227..22790
                     /locus_tag="VFJ72_00115"
     CDS             22227..22790
                     /locus_tag="VFJ72_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477893.1"
                     /translation="MDWLYRDLDLVERTIEECRAGNPSVHYQPGHPHGFHTQIYLGEV
                     HHCHPLHDPNYTLRSLKNLADPYPPCLKRTIVQKQLWEARFAVETCRKSAARGDAFYV
                     AGCLFRCAACLAQTLFALNERYVINEKGAIEVAGSLAVSPPYFADTIGSVLACPGESP
                     DLLQSSVRRLEELLEDVEALCRDQSSA"
     gene            complement(22775..23014)
                     /locus_tag="VFJ72_00120"
     CDS             complement(22775..23014)
                     /locus_tag="VFJ72_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477894.1"
                     /translation="MRFLSESRLGRWIVWVFYRAANKPLPVPHAAEWMMIPEHSVDQV
                     LETGALRSLYPQDVLEAARRMDRQRREHDERYAED"
     gene            complement(23057..23671)
                     /locus_tag="VFJ72_00125"
     CDS             complement(23057..23671)
                     /locus_tag="VFJ72_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477895.1"
                     /translation="MLEPDWDDTSSPQTDLRKMWWDIRFDPEPFENSAIDECLKALRE
                     TRIHGGILLGCFRVSEHKVFDWFASRNRLDEANFFRQFFVSPTVAVALPELRTEGLLL
                     DGDGFFGLPEFEWGSSFTLDGEIAEKLVIGGCHMSFPGTPTEAKELGDRFCKAIFGER
                     YTETQVYRSFDRWHEWFSYVEHLTWLLIDKRYRRIWLLCLTDND"
     gene            23894..24646
                     /locus_tag="VFJ72_00130"
     CDS             23894..24646
                     /locus_tag="VFJ72_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7477896.1"
                     /translation="MKLEGKVAVITGAGSGIGRATALKFVREGARVVVAEYNEEGGRE
                     TVQLCEEAGGEALFVYTNVAEFEQVEAAVDRAVEEYGSLDVMFNNAGTGHFAPLLEHD
                     PKDYDRVVRVNQYGVYYGILAAGRKMAALGVHGVIVNTASVFAFLASPGVFGYHAAKG
                     AVKMMTQAAALELAPHGIRVIAVAPGSVDTSIIQGYKDMGMEERLARVQMRGKLLQPE
                     QIANAVALLVTDEADAINGSVVMTDDGYAAFK"
     gene            24664..25032
                     /locus_tag="VFJ72_00135"
     CDS             24664..25032
                     /locus_tag="VFJ72_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET7477897.1"
                     /translation="MNRETTDFYEMVERALEGLPPEISALLDNVAIMVEEWPELSTPL
                     VGPDEDDTLYGLYEGTPLTERGWGYYAALPDRITIFRGPLERDFRPEDLEEQVRITVI
                     HEIAHHFGFGEDRLEELGYG"
     gene            25029..25463
                     /locus_tag="VFJ72_00140"
     CDS             25029..25463
                     /locus_tag="VFJ72_00140"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7477898.1"
                     /translation="MKKGRISIDQHIDAPAESVATYIGDFRNAKEWMVGVESVEKIGD
                     DLYRLSLESPIGKLSPEARVLEHSDHLLRWVYASTIDGGGSVEVSPGENGTCTVSYSG
                     EFRLKNKLLNRAAQAVGIERFAQMNGERSLARLKHLMEARRY"
     gene            25463..25651
                     /locus_tag="VFJ72_00145"
     CDS             25463..25651
                     /locus_tag="VFJ72_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7477899.1"
                     /translation="MGLFELSILLFVVFLVLGPKRITNLLRALGRGARDFKTEFGGKD
                     RDPELTEDDDRDANLRKK"
     gene            complement(25687..26631)
                     /locus_tag="VFJ72_00150"
     CDS             complement(25687..26631)
                     /locus_tag="VFJ72_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477900.1"
                     /translation="MDEPDRREVDGEREPDGMDAGTDGGADTSEVEVQREYGDDPVEL
                     KARLGIKDNHIRELYDQVSSSRLAADEAWASRQAGEGRVRALEDERARLKERIRTLEE
                     EARERRRGREGYERQIERLQRELERKDGEISRRDYLLERRAEELEAFSLDASDLITRK
                     DQALEDALRRVEGLERDLEERESEAAGLRATIDELRADLEEEYELRRRLAEPANRLRA
                     GIDLFNDSEQRRQMNALSRTLGQPEVHVSLDRGEEPPVLLAFTWRGITWQTYSVNPGL
                     DVEEPRIFLKSAGEDLSGVDREPPNGRVGPGGRVLLGL"
     gene            complement(26638..27933)
                     /locus_tag="VFJ72_00155"
     CDS             complement(26638..27933)
                     /locus_tag="VFJ72_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7477901.1"
                     /translation="MFTRSKTMPVPEVGAEAPEFNLPSAQGGQLRLSMRTVRGPVVVA
                     FYRPWSEEDVAYFRELAAKEDEINLAAGSVAAIGVAEPDEAREFARASGLKSYVLYDY
                     ARVTSRQWGLLEREKERGDYTRPAVFIVGPDGKVVHAWSDERPSAEELLSRVSEITGL
                     PKPAEEPKSEKTAGDAEPAAEGAEAEAKPAPKKLTAEEREKIKAERRAAREAGKSLKT
                     SQPEKADEGGAEGAAASDAPKKMSREERERIKAERRAARESGQSLKAPQPGKETGAGA
                     AGAEPAGAEPAGAEPAGAEPAGDGDDAPKKMSREERERIKAERRAAREAGLSLKTGGE
                     APRSEAADTPKPGAKDPDAGAGRPRDTSEAAKAAATASGTADDGSAEGSKVRSEASET
                     GASEPAQAEVTKAEPVEGEAAAVDEKESRKKTDGKPAEE"
     gene            28038..29585
                     /locus_tag="VFJ72_00160"
     CDS             28038..29585
                     /locus_tag="VFJ72_00160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7477902.1"
                     /translation="MTHGYMFSGSGSNVYVQNLCRALVREGHDVHLLCQEPAPLSYDF
                     VDEHRTANADGAHTHGEQETPYSGRCIVYNPEIGDLLPVYVYDEYPRWRVKTFLDLSD
                     EEFEDYVEKNAAALRAVLGASGAEAVVTNHSVPGPLIARRALEGTGVPYTSIVHGSCL
                     QYVSRRSETYMGVTREGLEGARKILALSSHSAGTIAEDFPEMKEKTLALPGGVDTGLF
                     RPDALDPGALKALIGGPGRGPDQRAALLHALDDRTSSAAELAKRLHKVEASYDGRSHD
                     SDAGERLGRLLEDGSPIIIYVGKLIHSKGVHSLISAFARIHRETGAHLLVVGYGTFRE
                     GLEALVHALGTGDRETLETLARGGALFEGGPEKRMEHFEVPESLLREASGMEDSVQFV
                     GPLYHAELAKLLPAAEVAVVPSIFPETFGLVAAEFAASGVAPFVADHSGLREAGGIVG
                     RGLPFDLRVGMDRFEEDLARALRDYLELPEEERRRCREVVRRNSVDYLSWNTLAEKVV
                     KIAGEKD"
     gene            29589..30824
                     /locus_tag="VFJ72_00165"
     CDS             29589..30824
                     /locus_tag="VFJ72_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HET7477903.1"
                     /translation="MEIEYILRDFGLILGAGLVSQLIARIIRLPEMIVLVAMGALIGP
                     SILGLVANPLGSVGSQLLFNIGVALILFHGGVGISLRVISKTAVGLGLLVLPGVLLTA
                     LIVALAVAPVFGVALPIALMVGAVLAATDPAILIPLFERIKLRPKVSQTVIAESAFND
                     PVGTVLTLTVAGVVSSGHFTVSGPALDFGRELVLGTLIGVGAGLILAVLVASSARIGI
                     WDEAPGVAILAVVALVYFSNEALHGSGYLAAFVMGLIVGNMDEFKLGQSEEHGKLLED
                     FAAQTAQIATLLVFVTLGLNLPFDALGKYFFGGLIIMAVFIFVARPVAVLACLLPDRR
                     GQWTWKEIIFLSWCRETGVVPAAVGALLLADKVPGAQLAVSMVALAVCATLLLQATTA
                     GFVASKLGLIEDGNSDTSG"
     gene            30892..31878
                     /gene="moaA"
                     /locus_tag="VFJ72_00170"
     CDS             30892..31878
                     /gene="moaA"
                     /locus_tag="VFJ72_00170"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET7477904.1"
                     /translation="MIQLTDSFNRKMDYLRISVTDRCNFRCQYCMPEDIQFQDKSHIL
                     TLEEMLTFAEACLQLGVTKVRVTGGEPLVRRGVVWFVSQLKAMGFEDVTMTTNGFLLK
                     ENLEGLVEAGLDRINISLDTLQPEKFAFITRRNHFERVWEAVMAALETPLSPVKLNAV
                     AMRGVNDDEISDMAKLTMKYPMHVRFIELMPLNGDTDGSRFRKLFISGKEILEKAQGE
                     LGDLRPIEKEDPAAPADEFKFEGAPGTFGVITPVSEPFCARCSRLRLTADGKIHPCLL
                     TDLDVPVKDAIRSADPIPAIHEVLWHTAGVKPAAGLTLPEEARNRTMSQIGG"
     gene            complement(31880..33343)
                     /locus_tag="VFJ72_00175"
     CDS             complement(31880..33343)
                     /locus_tag="VFJ72_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7477905.1"
                     /translation="MRLSGAGIAGAAVLGTAAGSSGCSNLWGGDTEMNVVVVVIDSLR
                     KDHVGAYGNRWIQTPNLDALSRESLMFTRAYPESLPTICARRAIHTGRRTWPFRDWKP
                     VKSDLFKMPGWQPIPEDQTTLAEVLSDSGYDTMFVTDTVHQFKPFYDMHRGFKAFHFI
                     RGQERDFFRPASAISETELDRVLEGGGVRQEATDIVRQHFANTRGRIREEDWFAPQVF
                     ITAMDFLSAAAQTEGPFFLLVDAYDPHEPWDPPENYIRLYDDHDGPKLMSSKSGRSAW
                     LGEKALRRMHTLYSAEVSMVDHWLGSFMEKFHELNLGENTLLILLSDHGVAFGEHGFA
                     GKVSEALYPELTDVPFLIRHPQGKKAGETTDYFASTHDVAPTVLGALGMRPSGPIDGQ
                     NLYALFEGDEMNARPHFTLGYHDHSWARDDRYALISQTSGAGAKLFDIQSDPGMDRDI
                     AGKHPEIVKRMFKDYVLEDAGGPLPDYGLEGSSNDSG"
     gene            complement(33802..>34063)
                     /locus_tag="VFJ72_00180"
     CDS             complement(33802..>34063)
                     /locus_tag="VFJ72_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET7477906.1"
                     /translation="ETTRRKMTTEPPYKVILHNDDYTPMEHVIQVLRKVIPRMSLRRA
                     VSIMLEAHTNGKAVAAKCHKELAEMYEEGLRAEGLIATIEPD"
BASE COUNT         5692 a        11591 c        10738 g         6042 t
ORIGIN      
        1 gcgtacaccc cgcagtagat ccagcagccg caggaggtcg agccgtcctt cttcagctgc
       61 tcgtacgtgg acagcggcgc gccgtcgacc gtgaagccgt tgatctccgc gagcacggcc
      121 tcggcgctcg gctcctcgat ctgcccgtgc gtggggtagt cccaacgcag cttccggatg
      181 ccctcgtcgc gcggctcctt ggagccggcc agcttctcct ggatgcggcg gccgaggtgg
      241 tagtagaacc acagctccga ccggcagtcg ccggggggct cgatcgcctg gtggtgccac
      301 tggagcagcc gctgcgtgtt cgtgaaggag ccgtccttct cggtgtgcgc ggccgccggc
      361 atcaggaaca cctcggtcgg gatcgcctcc gtgcgcagct cgccggtctc gatctccggc
      421 ccggccttcc agaaggtcgc cgtctcgatc tcgacgaggt cgcgcacgac gagccacttc
      481 agcttcgcga acgcgagccg gtgcagccgg gagttggcgg agccgacggc ggggttctcc
      541 cccgccacga tgtacccctc gacggtgccg tcgagcatgc cgagcacggt ccggtacacg
      601 gagtggtcct ggtcgatccg gggcaggtag ccgtagcccc agtcgttttc ggccgtcgcg
      661 gcggcgcccc agtaggcgcg catcaggctc acgaggtact cctcgatgtt cgcccagtag
      721 ctcgtctccg agcggttctt cgcgacgtag tccgcgaggg tgacgtcctg cgtcacatgc
      781 ggcatcggca ggtagcccgg cagcaggttg tagagcgtcg ggatgtcggt ggagccctgg
      841 atcgacgcgt gcccgcgcag ggccatgatg ccgccccccg gtcgacccat gttgcccagc
      901 agcagctgca ggatcgaggc ggcgcggatc ttctgcaccc cgtagctgtg ctgggtccag
      961 ccgacggcgt agcagaacgc ggaggtccgc tcccggcccg agttctcgca gagggtctcg
     1021 gcgacttcga ggaacttctc tttgggacag ccgcagatcc tctccaccat ctccggcgtg
     1081 tagcggctgt agtgcttctt gagtagctgg aagacgcagc ggggatgctc cagcgactcg
     1141 tccatttcgg tgggctctcc atgctctagc tggccgccct gcgcgtagtg ggcctgctct
     1201 ccggtcacgc cgccctgctc gcgcttgccc gccgagccgc tgacctccat cccggcgtac
     1261 tgccaggtgt ccacgtcgta ggagccggac tccgggtccc agccggagaa gagaccgtcc
     1321 aggtcctcgg tgtcggcgaa ctgctcgttt atgatcacgg gcgcgttcgt gtaatgaaca
     1381 acgtagtctc tgaagtactt ctcgttctcc aggatatggt tgatgatgcc gcccagaaag
     1441 gcgatgtccg taccggcccg tatggggacg tgcaggttcg acatcgcgct cgtgcgggtg
     1501 aagcggggat cgacgtgcat gatcttcgcc ccccgctctc ttgcctccat cacccactgg
     1561 aaccccacgg ggtggttctc cgccatgttc gagcccatga tgaggatgga gtcagcattc
     1621 gcgaggtcct gctggaaggt cgtagcgcct cccctgccga acgaggtccc gagactcggg
     1681 accgtggagg agtgtcatat ccgggcctgg ttctctatct gcacagcccc cattgccgtg
     1741 aagaacttct ttatcaggta gttctcctcg ttgtctaggg tcgcgccgcc gaggtgggcg
     1801 aagcctaagg tgcggtgaag gtgcctgccg tcctcgtcct catcctgcca ggtctcgtcg
     1861 cgggtcttga tgagacggtc ggagatcatg cccatcgccg tctccaggtc gagatcctcc
     1921 cacccggttc cgtacgggcg gcggtacttg atcttgtact ccctggcggg gctctggacc
     1981 agactgcggg aggcggcgcc cttcgggcag agcctgccgc gggagatggg agagtcgggg
     2041 tcgccctcga tctgggtaat ccttccgtcc ttgacgaaca cccgctggcc gcacccgacc
     2101 gcgcagtagg gacagacaga cttcaccacg ccgtcggccc cggaggtgag cggcgcgagg
     2161 gaggccgaac gcgccgatcg cgcggagctg ccgagtccga ggtagtcctt gcctctgacc
     2221 tggcgcagta ccggccaggc tgctatcgag tcccttatgc tcaaccgtcc tccttcgcca
     2281 cgaatcccgt aacggtccgg cggctatcgt gtccgaagcc gcctcgacac gacctggccg
     2341 gcgagacaat tagtgtcccc cgtaaatagc acgcccgacg cttttcgcgg cgaccgccta
     2401 cgtagctttc ccggacagtc gtcctctcaa gcgtccctta tgggagatca tgctacgtgg
     2461 tccggaggat tctgaagtaa agatgatgta aagggagggt aaagcttctg caagaacacc
     2521 ttccgagcgg ggctatcttg acgtaatcga gcacagccgg gagaacacgt ctcaggatct
     2581 ggctgctgcc gatccctcag cgctcgctga tgtgcggcgt tctatctcct tgcggacggc
     2641 gggggcgacc tcggcgccga acaactcgat ggcgcgcatg accttctcat ggggcatcgc
     2701 gccgacactg atctgggcca tgaagcgctg gtttccgaag agctcgtgct cgtagaggac
     2761 cttctcgacg acttcctccg ggctgccgac gagtagcgcg ccgcgcggcg agcggcccat
     2821 ctcaaactgc ctgcggccca ttggccccca cccgcgctcg cggccgatgc ggttcatcat
     2881 cgcggcgtag gcggggaagt actcgtctcc cgcctgccgg gacgtctccg cgacgtaggt
     2941 gtgcgagttt atgctgatcg gcaaccggga ggggtcgtgt ccggaccgcg ccgcggcctc
     3001 gcggtagaga cgggcgagcg gcgcgaagtt ttcgggctgg ccgccgatga tcgcgatggc
     3061 cagcggcagg ccgagcgccg cggcccgcac taccgattgc ggcgtcccgc cgacggcgat
     3121 ccacaccggc aaagggtcct ggaccgggcg cgggtagacc ccggcgtctt cgagcggggc
     3181 gcgatgcctg cccgaccacg tcacccgctc ggactcgcgc agcttcagca gcaagtccag
     3241 cttctcggcg aacaggtcgt cgtagtcgcc caggtcgtag ccgaacaaag ggaacgactc
     3301 gataaacgag ccgcgtccgg ccatgatctc cgcgcgcccg cccgagatga ggtcgagcgt
     3361 ggcgaagtcc tggaagacgc ggaccgggtc gtccgaactg agcaccgtca ccgcgctggt
     3421 caggcgtata tttctcgtgc gcgcggcagc ggcggcgaga gccacggccg gcgacgagac
     3481 ggcgtaatcg gggcggtggt gctcgcccac gccgaagacg tcgaggccgg cttgatccgc
     3541 cagttcgatc tcctcgatga ggtttcgcag ccgctgctcg ggggtgatct cgggtccgac
     3601 atcggcgaac gtatagaggc cgagttccat tgtttcgggg gtcctttcgg gtcgcgtcct
     3661 acggaaacgg atgtccggcg acggttctga gcatctggcc tacgccatct accagaggtc
     3721 gttgcgacgc atgaggtccg tcttctcgtt cgggctgggc ccggtcacga cgaaggcccg
     3781 atctttcgac cgggccctct ctgtattgta gtcttccggt acctgggcgc tccctagctg
     3841 agagatagcg gccagtcagc cgcgccgtac atgagcgcca gggccgcgcc cgtcagcgcg
     3901 atgttcttga ggaagttgac ctgctccgcg gcccgctgca tcgggtcgct gatggtccag
     3961 aagttgtgca tcagaaacga ggtcgggatc aggaacagga tcaagagcag caaccccagt
     4021 accggcagga agccaagcag gaccgaaagc ccgccgagca ccagcatcac cccggtcccc
     4081 atgaccgcga gacccggagc gggcacgttc ttcgagccag cgtaaccgct tatcatcccc
     4141 actttcatga agtggttgat cccggacata ataaagaacc cgccgaacag tatccgcccg
     4201 ataagcaaca gtagctccac gatctccctt tctttctaaa cgtctcttac atttgtaact
     4261 tatattatat attggaccca ccggaccagg tgtcctcgga cagcccggtg gagcatcttt
     4321 aaacctggac cccggcgagg agcgccgcct tctcggccac cctcttgccc tggtagcgcg
     4381 ccgcggcgag ctcctgctcg ctgggaccgc cctcgccgcc gtcggtgctg ctgacgccgt
     4441 aggggttgcc gccggactcg aagagcaccg ggtcggtgta gccgggcgtg accaggatag
     4501 cgccccagtg catgaacacg ttgtacatgc ttaggagcgt ggactcctga ccgccgtgcg
     4561 cgttctgcgc gctggtaaaa ccgctgacga ccttgtccgc gagcttgccc tggaaccaca
     4621 gcccgccggt ctgatccagg aactgcttga gctgcgccga gacgttcccg aaccgggtcg
     4681 gcgtgccgaa gatgtaggcg tccgcccact ccaggtcgtc catcgaagcc tccggcacgt
     4741 cctcggtcat gagagcgtgg tcgtaccagc cctggttgct ctttacggcc tcctccggcg
     4801 ccagctcgtg caccttccgc agccgaacct ccgcgtcggc ctcctccgcg ccctccttta
     4861 tcgcctgggc cagcttatat acattgcccg tcgcgctgta gtagatcacc gctaccttga
     4921 ccgacattgc tctcctctcg ttaaaacttt cactaaagtg gacccacgtc cactttcagc
     4981 tctgaggcta acgcggtaga ctgaccgtgt caagggttca ggagcagatg gtgcaggaga
     5041 tcggcaagaa atacggcgag atggagtggt cactggacca ggccggccgc gctctgccgc
     5101 cggggacgga gcgtaaggat gcggcgcgta accggcgtaa gatcgtcgag acggcgcgcc
     5161 ggttgttctc cgagcgcggc gtaactcagg tgagcatgga ggagatctcg cgtgccgccg
     5221 gagtcgggaa aggcaccctg taccgcaggt tccccaacaa gggcctgctc tgccaggcgt
     5281 tgatggacga gccgacgagg gagttccagg aggagaccct gcggattctc ggcgacgccc
     5341 ggcgcggtcc gctggagaag ctggacctct tcctcgacgg gctcgtccgc ttcaccgacg
     5401 agaacctgga cctgctctac gggggcaacg agccgctgtg cggcgcggac cggctggccc
     5461 ggttcgagca tccagccttc gactggcgcc gctggaccgt cctcgggctc ctgcgggcgg
     5521 ccgcgcgcga gggagacctg aacgagaccc tggacctgga atacctcgcc gccgcactcc
     5581 tcgcgccgct cgacgtggac ttcttctact ttcagcgccg ggtgcgggag atgacgacga
     5641 agaggatcag cgcagggcta cgttcgcttg tgcccacata gagaggcgca aagagaacag
     5701 taaaactgga ccaggaagac ctataagaaa ggaacggaga gaaaatggaa cttggcggat
     5761 tgcatcacat gacggcggtg acgacggtgg ccgcgaggaa cgtggagttc tacacgcggg
     5821 tgctcggcat gagactggtc aagaagaccg tcaaccagga tgacacatcg gcatatcacc
     5881 tgttctacgg cgacgagctg ggccgggcgg ggaccgaggt cacgttcttc gactggcccc
     5941 agttgagggt cgcgccgaac caccccggcg cgggcgaagt ctcgacgacc gagttgcgcg
     6001 tgaggggacg cgaggcactg gattactggg cggggcgcct ggaggagttt gaagtagctc
     6061 accacggcat cgaggagcgc gacgggcggc tcgcgatcag cttcaccgac cccgagggac
     6121 agcggttgcg cctcgcggac gatagcgcgg ccgtcgtcga gggcggcaga cagtgggaga
     6181 agagcgacgt cccggcggag ttcggggtgc gcgggctcgg ggctttcgac ctcacggtgc
     6241 gcgaggcggc cccgaccgcc tgggtgctga ccgacatcct gggcttcagg gagtcggccg
     6301 agtacgagga ggacggcgcg cgggtccgcg ctttcgaggt cgggccgggc ggaccgggag
     6361 cggtggtgcg gctcttcgag aggccggacg ctccgtgggc gcacctggga cgcggcgggg
     6421 tgcatcacgt cgccttccgc gcgccggacg acgagcagca cgaggcttgg cgcaagcgca
     6481 tcaggagcgc cggcatcggg gtgacgcccc agatcgaccg ctactacttc cgctccatct
     6541 acttccggga gccgggcggg gtgctcttcg agatcgccac ggacggaccc ggcttcgcca
     6601 ccgacgagga cgccgcccac ctcggagagc ggctctcgct gccgccgttc ctcgaagacc
     6661 ggagggagga gatagaggct cgtctggagc cgataatggt ccattgaatc caacgcggtt
     6721 atagtgaatg cttcgtaaag tagagcttta gcggaaggag aggtaatggg taatctttcg
     6781 gcgttgttgt tgcggctggt cctgggaggg ctcctcgcgg gccacggggc gcagaagctg
     6841 ttcgggtcct tcaacggtcc cggcctggag ggaacgagcg ggttcatgga gatgctcgga
     6901 ctcaagccgg gcaagccctg ggcggtcatg gccggtctct ctgagttcgg cggcggcgtg
     6961 ctcacggtgc tggggctgct aaacccgctc ggccccattg gggtcatagg cgcgatgtcc
     7021 atggcgacgg ccaaggcgca ctggggaaag ccgatctggg tgaccgaagg cggcgcggag
     7081 ctgccggtga ccaacatagc cgcggcgacg gccctgatcc tgaacggtcc gggcaagtac
     7141 tcgctcgacc gcgccctggg actcaagctc ccgtcttggc tcgcgccgct gggactcgtg
     7201 gcagtcatcc tcacggtact ctacgccggc atcggcagcg gcgcccagcc gccggaggcg
     7261 aaccaggaag aggctcgcga agacctcgcc ggaccggaag agtaaccgtc cgtcctgata
     7321 cgaacagtaa ggcacacacc gacccgcagt ggctccgctg ggtcaagcgg ctgcaagcca
     7381 tagcccagga cggcctcacc tacaccgaag atagctacga cgtcggccgc tacgagcaga
     7441 tccgggagct agcggccgac atcctcgcct cccactcgac cggagacctg aacggcgccc
     7501 gcgagttact cgctctacag tccgggcccg ctacccccaa ggtagacgtg cgcgccgccg
     7561 tcttccggga cgggaagata ctcctcataa aagagccgga ggacggtctc tggtccctgc
     7621 cgggcgggtg ggccgacgtg ggcgagaccc cgtcggagac gacggcgcgt gaggtatacg
     7681 aggagtccgg ctaccgggtg cgggttgtga ggctcctcgc cgcctacgac cgcgaccgtc
     7741 agggccacac gcccctcccc taccacgtct acaagctgtg tttcctgtgc gagatcgtgg
     7801 gccacgattc ctcggtagcc gtcgaggccg acggcatcgg gttcttcgcg gaggacgacc
     7861 tgccgccgct ctccgcctcg cgggtgacgg aggcccagat ccagaccttc ttccaccagc
     7921 accgccatcc cgaagccccc gccggcttcg actgatggtc agcccccgac ccgcagcacc
     7981 gccttgccgg tgaagcctcg ctgcatgagc cggtcggcga ccgccccgat ctccgcccag
     8041 tcctcctcga cctcgattcg cggcatcagg ttcccttctt ctatgaggcg caccaatctc
     8101 gcgagcccct ctgatgccgg gtgagccagg acctcgtgga agatgatgaa cccgtagagc
     8161 ttcgcgccgc cggtccagaa taggctccgc acgtcgaagg tacactccgc gccgcccgag
     8221 acaccgaacg agacgcaggt cccgccggtt gcgagcatag agagcgcgtt tccgagcacc
     8281 gccccgccga cggactcaag gaccaggtcg tagggaccgt actccctcgc cgccgccccg
     8341 tcctccccga cggcgacctc atgcgccccg gcatcccgga cgagccgaac atgctcctcc
     8401 cggcgcacga gcgccacgac ccgcgccccg gccagccgcg cgagctgcag cgcgaagagc
     8461 ccggcccctc ccgaggcgcc cgtcaccagc acccgtctgc cgaggagcgc actgcctttc
     8521 tccagggcgt agagcgccgt gagaccggcg atgggcagcg tcgaagcctg ctcgaacgag
     8581 accccgtccg gcagctccgc gagcgcgttc gccggtactg ccgccagctc cgcccacgcc
     8641 ccagccggca ggaacccgac tacccgcgct ccctcggccg gtcccgttcc atcggcggcg
     8701 gcgcgctcca cagtcccggc caggtcccag ccgggacggg ctccgggctc gtcggcgccc
     8761 gtcgcgcggc gcacctcgcc ccggttgagt gagaccgccg ccacccgcac cagcgcctcc
     8821 gacggagccg gcgccggctc ctccacatcc gagatcgcca gccggtgcgg ggcgttcggg
     8881 tctacgacta cggctcgcat cctggtcacg gtctctctcc tatccggtcg gtacgttgct
     8941 ccgcccgtag tctagcggag agcttccagc gcctcccagt cctggccgtc cggtataatc
     9001 gctccatgca acccgccgcg acaaagagcc ttccggagaa cgtggacctc atggtcctcg
     9061 acgcatccgg cgggctgcgc gaagaggtcg ggctggagga gcttcccgga tatctggcgg
     9121 acaagcgcgc cctgatctgg tgcgacatct acagcacgca gggcggccag cacggtccct
     9181 acgggcggct gcttagcggg gtcttcgggt tcgacgaact gaccgtagag gactgcttca
     9241 ccaggagtca tctcccgaag gtagacatct atgaggatta cctcttcgtg gcgctcttct
     9301 ccttccatct ctcggagaag acccgcaggg tccagaccgt cgaggtggat atgtacttcg
     9361 gggagaactt cgtcgtctgc gtacacccgc gcccgcttag ggagctggag cgggtgcggc
     9421 gccgactgct ctccggcgat gagttcgtct catcctcgcc ggccaacgtc gcccacacgg
     9481 tcttcgacgc ggtggtggac gagtacctgc cgatcatgaa ccggctctcc gcgctggtgg
     9541 acggcattga ggacgacctg ctcggcgagg acaacgacgt ccccgaggac gtgctcgacc
     9601 acctcttcca tctcaagcac gagcttaccg cgctgcggcg cctcgccgtg ccgctgcggg
     9661 acgtggtcag catcctcatg aggcccacca tcaggctcat ccccgaggag agccggatgc
     9721 actacgacga cgtgcgcgac cacctcgtgc gggtcataga catgatcgac accatgcgcg
     9781 actaccttac cgggtcactt gacatctaca ccacccagca gacccagcgc ataaaccaga
     9841 gcatgcagcg cctgaccgcc atctcgctaa tcttcctgcc gctcacgttc ctgaccggca
     9901 tctacggcat gaacttctcc tacatgcccg agaccgagtc ccactacggg ttctacgtcc
     9961 tgatcacgct ctgcgccctc gtcggcctgg ggatgctcta ctacctccac cgcaagagga
    10021 tgctgtaggt gctttcccga acggctacag gttgaggatc accacgtcgc acagcgcatg
    10081 gtccaccaca aagcgcgcgg tatgtccgag aggtctctcg cccggcatcg cctcgggaga
    10141 ttcgtgcgcc tcgatcggga cgccgttgcg cgtgtcggtg atggagtaag cgtgcaggac
    10201 gcgcctcccc tgccaaacgc ctgacgcctc gatctctgcc ccgtcgctta tcccggagcg
    10261 cacggcttcg gcccggtgcg gcggaaaccg gacctcggtc ccgtcgccga gaacgagacc
    10321 gtcgaacctg ccctcgcggt tctggttcca gccttcgaca atgcctgaaa ccgtctccct
    10381 ggaccgggat ccgggcgtcc cccggctgat gaagatggtc tgcgcaccgg cttcgcccgc
    10441 ggcgcgcatc aactccttgt tcgggtcgcc gtccagaacc ctggtctcga tcatattacc
    10501 tgggcggagg cgctggagga gatcccgcgc gtctcccagg aacccttcgg cggcctcccg
    10561 cgaggcgcgg accatcctct cttccgcgtc gcccggccgg cgccttccga gcaaccctcg
    10621 cagcgacatc tcgtagccgc gaggagcccg ctcgtccacc acatgagcca gggtggccgt
    10681 cccttcgtca gccaggtaac gagcggcgcc ccgtaccacc ccttcccagt cccggccatc
    10741 aacgcacaag aaagccttca tactcctcct tctctctgta ttccagtcta ctccctcctt
    10801 acgtaccgag cagcgcgacg gagatccaca accccacggc agcggccagc aggatcgcgg
    10861 gcgcggcgat tgccccgatc ttgaggtagt ccttcgcggt tatgtccagt ccccgcccgc
    10921 gagcgatgct aagccagatc agggtcgcca gcgaacccac ggtggtcagg ttcgggccga
    10981 tgctggtccc caccaccatc gcgtagaccg ccgagactcc gagcgtcccg cccttgacga
    11041 gagactccat cgtgcccagc gccaggaccg tcatgggaac attgttgatg atgttggcgc
    11101 cgatggcgct cccgaaggtc agggccagga tctggaagaa gccatccccc ggagccgccg
    11161 ccgcgaaggc gctctccaca agtcccgaga gacccgcgtt ctgaacaccc tgcaccacca
    11221 ggaacaggcc cactacgagg aagatgatgc cccaggagac cgatcccacc acctcccgca
    11281 gcgttaccca gcccgccgcg cgcgacacta tcaccacgat agcccccgca gcgagggcca
    11341 cgaacccgat cggtatcccg agaagcggcg ccgcgaagaa cgccacaagc acgcccccga
    11401 tccccgccgc ggctacccgg aacagcccct tgttctccgc ctcgaaccgg gctcccttgc
    11461 ccaggtcgta gttgccgcga aggtcgttgc ggaagaggaa gacgaagatc gcgaagttgg
    11521 tcaggatcgc cagcacgccc ggcagcagca tgacgagccc gaaccgcgcg aagcctaggt
    11581 cgaacctgtc gtaagccaga aggttggtga ggttggagat ggggaggaag agcgaagcgg
    11641 tgttcgccgt gtagacgcag gcgaacatga agggcatggg gctcaggcgt aaccggacga
    11701 ccatgccgta gacgataggg gtcagcacga tcgccgtggc atccagggag agcgtcaccg
    11761 tgacgagcgt gccgaccagg aacacgaacc cgtacagcag gaggacacgc tccctgccgg
    11821 cccgggcggt tatggaggcc gcccagtcga agaagccggc tttctccgcg acggcggaga
    11881 gggacatcat tccgatcaag aggacgagcg cgtcctgggt ctccagcagg atgtcccaag
    11941 cctgcccgag agacaccagc cccagcacga gcgtcagacc gccgccgagc accgcccacc
    12001 aagcctcccc gatgtcccag ggccgcgtga gcacgagccc cagggtggcc gcgaggatca
    12061 cccccgctag caccgcgctc acgcggcggt ctcccgaccg gcaatgagcc cggtaaaggt
    12121 tctcgggagg gcagactgga gacgatgctc gaccggcggc atcttacggt tatccctggg
    12181 accgcagctt gtccagctcg taacccatca cgtgtccgca gttctcgcag acgaggtccg
    12241 cgcgcttcag acgccgggtg ccgtagcccg ttcccgcggc gtccatcggc tcgacctgct
    12301 ccagggaaag gtcctcgctg ccgcactccc tgcacacggc cgcgccacca cgcgccctga
    12361 tcgcctgccg cagatcatcg gccgaagggt gggaagatat gttcataccg gaacctcctt
    12421 ttggtttcgc cgtggattcg accccgcagc atgacattca gacgtcgtgg cgcgcgaata
    12481 ccagggctcc tatctagggg ccggggcgag gtggtttgtg cgcttccacg cgggtcccgg
    12541 attccgtgtc ggcgatagtg taggcatgga acctctcgcc gtgccactcc ccaacggcct
    12601 cgatagcggt accctcccga aaaaggtcct ggacctccgc ggcccggtgc ggggggaatc
    12661 cgatctcgcg cccgtctttc agataaaagc cgtcagtgtc cccgtgatgg ttggccttcc
    12721 acctcgcaac ggttccggag acggatgtcg ccctggaacc gaaagaccgc gccgcgcgct
    12781 gcggcggagc gggcgggatg gcgcgctcca tctcctcgac gaagtcctcc ggcctgtcgg
    12841 aggatgccca tccggcatac tgctcgatgg cctcgtctac cgccgccttg agaagctcct
    12901 ccgcaagagg tgtcttggca atgtccaggt tcttcgccag ccagaccagc ttggcatgat
    12961 cacgctcgct caaccgcatc gagaacgtca ccggcttcgt cggcctcctt ccggatcttc
    13021 cctccatctc ctctatgtac tgcatcatta gacccttcac cttgtcgccc atctactcct
    13081 ctcctttcca ggttcgcagc ccgtagcttg acagctatac accccagccc tcctcccgta
    13141 accgtgtaat acacagtttt gtactacaac tgtattattt attcaagtat tttatttatg
    13201 acaccgtttt atgtcggttg gtggtgtggg aaagggaccg aagcggcatc tccgatcccg
    13261 aaggtcctgt atagacttgc cgggagacgc gagcgacgga cacttctcgg ggcgctggcg
    13321 ctttacaacg cgtggcgcag cgactataca acccggagtg agcaagacgg gcgacagcgg
    13381 agagcggcgc gaacaacctg gtgagaaccg gagccgggga aaggcggggc gggagagagt
    13441 gcgcttccgg gaccggggcc gtaagggcgg cgtgcgagac cggctgcgga accggccgca
    13501 catccggccg aaggtctatg ggtaccttac cgaggagaaa gagtccctgc ggcagggttt
    13561 cgcggcgctc ttcctctcat cgaccgggga attggtcgct ggtctggcgc tcgccagcat
    13621 cacggagacg ctcgatcaac tcgtcgggct cgcggtactc atcccggcgg ccatcgggat
    13681 gcgcggcgcg atcttcggcg cgatggggag ccgtatctcg accgctatcc agacgggcct
    13741 cttcagcttc aatgtccggc gcggtgtgct ggccgagaac gtgcaggcgg cagcggtact
    13801 ctcattattg tccggcatct tcctggcatt tctggcgcgg ggtttctgcg gggtgctcgg
    13861 tgttcggacg gagctatcgg tcttcgacta tgtggtcatc tcgacggtcg gtgggctcct
    13921 ggcgggactg ctgctgctcg gaatcaccat tatcgtcgcc aggttgtccg tggcgcgcgg
    13981 gtgggacatg gacaacatcg cggcgcccat gatcacggcc gccggagaca tcctcaccct
    14041 tccctctctg gtgctggcga cgtatcttat aggcatcccg gtattctcca gcgccctggc
    14101 cgccgccctg atactctgcg ccgccgtcat agtcacgttg gccttccggc tcggcgcggc
    14161 gaacctccgg cgcatcctgg ccgagtcgct ccccatcctc gccctcaccg gcatggtgac
    14221 catcctcgtc ggtgtcgcgc tccagcagag agaggagcag ttctcgacgc tgctggcgct
    14281 ctcgatactg ctgccggcgt tcttgcagga gggcggcgcg ctcggcggca tcctctccag
    14341 ccggctctcc tcgaaggtcc acctcgggct tctggccccg cgcggcatcc ccgatctcgc
    14401 ggcgttcaga gacttcacgt tgacctacat cttcgccgcc ggagtgtacg tgttcatcgg
    14461 aggggcgtcg cacttcctgg cggtggggtt gttctcgact gaggccagtc cggggttcgt
    14521 ggcgatgctc gggatcagcg cgctcgcggg gctcatagcg acgacggcgg cggtgttcgc
    14581 ggcgtactac gggtccacta taagctaccg gctcgggctc gacccggata cctacggtat
    14641 cccgataatc acggcggcgg tggacctgct cgggttcatg agccttataa tctctcttgt
    14701 agtcttcgga ttggtgggat agacacatgc gcgatacgcg gcgcggaacg caacccagga
    14761 acctcaagga catgctcgct gaagcgaaga acacctcgga gctgatggtg gacctcgcat
    14821 acgcggcgat cttctacaac tcggaggact tctccgagga ggtcctgcgg ctggaggagc
    14881 gtctcaacga cctcgtcttc gatatgcgca ccctcgcgat actcgccgcc cgcagcccca
    14941 ccgacgccga gcagatggcc ggcatcctcg gcgtcgtgga ggacatagag aagatcggca
    15001 acgccgccat agacatcgcc aagatcgtgt tgaagcggct cgggataccg caggagcttc
    15061 tgcacgacat cccggaggcc gaggagatcc cctctcgggt acgcatccac gccgagagcg
    15121 cccttgacgg ccggagcctg gaagaggtgg acctcccgac cgagaccggg atgcgcccca
    15181 tcgccatccg ctccggcgac gagtggaact tcgaccctga gcccgaggac atcctccgcg
    15241 cccgcgacgt actgttcatg cagggaccac cggagggcgt cgccgaggtc cgggagctcg
    15301 ccggggcggc tccgctggaa cccgccccgt ctgtcgaggc cggagcgtta tccgagctgg
    15361 agcgggccgt ggacatcctg atcgagatga agaacctctc ggaggtcgcc gtgggtctcg
    15421 catactcggc cctcctctac tacgacgccg ggctcgcccg cgaggtcgtc gccatagaga
    15481 acgagatgga cgagatgcgc taccgtctcg aacgctgggt gctcctggcg gcccggcaca
    15541 tcgaggactc gtcgaggctg cgtggagtgc tgcacctggc gaccgcctcc gagaccatcg
    15601 ccgactgcgc gatggagatg gtctggatcg tggagaaggg cgaggaggtc caccctgtcc
    15661 tctccgccgc tgtcggcgag tccgacgagg tagtaatgaa gctgaccgtc tcccccggct
    15721 cccccgccga cggcaggagc ctggaggagc tggagttcga gaccgagacc gggatgtacg
    15781 ccctcgccat caaccgcggc ggacgctgga cctaccggcc tcgcgacaac tacgtactgc
    15841 tcggcggaga ctccgtcctc gtcaccggcg cccccgaggg cgtcgaaccc tttgccgaac
    15901 tcttcggcca ggagctggag gagaccccat aacaggtaag cacgcagcgg accggaccgg
    15961 gggagccttc ttctggcttc cgctggccgt cctcgcggcc gccatgtacg cgctgctctt
    16021 tctgcggttc gcgggtctac cgccggacct gaaggacaac tcgaacgacc tccgcatcta
    16081 ccgggacacg ggagaggccg tgctgcgcgg cgagttcccc taccgggact tctttatcga
    16141 gtacccgccg ggaagcgtac cggccttcgt gccgcccgca ttcttctccg actccacgag
    16201 cggatacatc acgcttttcg ccaacgagat ggcgctcttg ctcgtggcgg cgctcgcgct
    16261 ggtcgcgctg gcggcgcgca agatgtggac aggatattca tggtccgtgc cagcgcttac
    16321 gttcgccgcg tcggcggtcc tgctctaccc ggtggccgtg acccgctacg acgccgtcgt
    16381 cgccctgacc ctggcggtgg ctacgtggtg cgcggtccgg ggcggacggt ggcccgttat
    16441 cctgggctat gcgtcgctgg ggttcggagc ggcggctaag ctggtgccgg ctttagcggc
    16501 gctcccgctg gcgctcattc ggcggcggag cgcggtcccc ggattcttcg tgttcttcgc
    16561 tgtggtggcg ctctttttcg gaccggcggt gttgctggcg ggcggacagt tcgcggacag
    16621 cttcgcctac cacgcgcagc gcgggttgca ggtggagagt gtcgggtcgt cggtgttgat
    16681 gcagctcggg tggatcaggg agatagtgtt cgagttcggg gcgttcgagg cgcggggccg
    16741 gggaacggag ttcgccagct ccatcagcct gcccgtgacg gccgcgctgc tcgggcttac
    16801 ggcgctcgtg atgtacagga agcaccggga gggcggtact gacgccgggc tgtttgcacg
    16861 ccatgcggcg gcgcttatac tggcgttcat gctcggctcc aaggtactct ctccgcagta
    16921 catgatctgg ctgctccccc tcctcccgct ggccgcgccg cgcctcacgg gactcggcgt
    16981 ctcggcgctc ttcctcgccg cctgctggac gacgacccag gtcttccccg tccactacaa
    17041 cgacctcctg accctccgct ctcccggccc ccaactgctc ctggcccgca acctgctact
    17101 cgcgttcctc tgggtcctca tgctcctgtt gcccacgagc gcgccgactg gcgaacgctc
    17161 ggataaggca gcgtcatgaa gcgatccgcc atactcctcc tcatactctt gctcctgccg
    17221 ctcctgtcgg tgacgctcgg gctgcggggc ggcacccatc tggacgagca acaggcgcgg
    17281 cagagggcgg agcttgcccc gaaggtcgcg aacctggtgg aacatccgag cgtgcagccg
    17341 tcggcgacct atgactcggt ctcggacacc tggctcgtgc tgctcacgga agaggtctcc
    17401 gggagcgtgg tggcgaaggt cacggtagat gatgacaccg gcgacgttgt ccggacggag
    17461 atctccccaa acgccaccgg tatctcgtat ccgagcctct ccgaagagga cgcgacgaag
    17521 ctggcgacgg cggacatacg ggtccgggac tacctgtcgg acttcggctc ctacagcacc
    17581 gatacggagt acgagaatgg cgagtggacg atccactacc gcatccaggg aaccggggcg
    17641 gtcggcggcg tcccggaccc ggaggcgggg accaaggaga tagtgcgggc gggcgtggac
    17701 gacgacacct gggtcgtgga gtatgtgtgg gccggagacc aggtcggctg gcagatggcc
    17761 cgtggcgagt acggctcata cggcaagcag gccaactact ggtacgtctg gggaccgatg
    17821 gcgctgctct tcgccctcgc cttcgtccgc gcggacaaga tgttctccct gcgcaacctg
    17881 gacatcttaa tgctgctcgg gttccttgtg tcgcacggct tctggcgggc gggcgactcg
    17941 tacgaggcgg tgctgctctg gtatccgccc ctgatctacc tgctcgtccg caccctgctc
    18001 atgggcttcg gcatcgggga gcgcgtggag aagacggcca acttccccac ccccgtcctc
    18061 ttcatcctcg cggccctcgc cggaggcttc gtactcgccc tgaacctgga tgcccgcgtc
    18121 atagacgttg gctacgccgg agtcgtcggg gccgaccgca tcctcgacgg caccctcccc
    18181 tacggccaca tgccgagcga cgtggggacc ggcgacacct acggcccgct caactacctg
    18241 ctctacgttc ccttcgtcct gatgttcggc ttctccggca agtgggacta cctgcccgcc
    18301 gcccacgcca cgaccgcctt cgccttcact gtgggcgcgc tggcgctgct ctacgcaggt
    18361 tacaggctcg cgggggcgcg cggggcggcg gcgatggtct tcgcctgggc cgtcttcccg
    18421 tacacgctct actcgaccaa caacaacacc aacgacgtaa tcgtcgcggc ggcctcggcc
    18481 gtgagcctgg ccttcgccac ctccccgctc gggcgcggcg ccagcatcgc cgccgggttc
    18541 gccataaagc tctacccgct catcctcgcg ccgctctgga tggcccaaga ccgctggaag
    18601 agaagctcca tcgtggactt cgcgctcggc ggcgtgggcg tgtgcctttt gacgttctgg
    18661 atcctgacgc tcgacggcga cataatcagc gcgctcaagc tcttctacca gaagaccata
    18721 accttccagg gcgaccgcga gaccccgtgg accatcttcg cccaggtccc gcagatagcc
    18781 ttcctcaagc ggctgctcac ggcggccgtg atcttcctgg ccgtcctcgt cgccatcttc
    18841 ccccgcaaac gcaccgtccg ccgcctcgcc gccctctccg ccgcgctcgt aatagccgtc
    18901 cagttagtta ccaactactg gttctacacc tacgtcgtct ggttcgagcc cttcgtcttc
    18961 ctggcgctct tcaccgccac gaatgaaaaa acggctctgg atggtgagac ggaaccgccg
    19021 cccgaagcca gcgtcccgca gaacgccggg accggcggat aacccttacc gttcgcgttc
    19081 ctcgcgcgcc ttccgccacc gatccagcct ttccttgatc ctcgcctcca ccccgctcac
    19141 tttcggctcg taatagattc gtccggcgag ttcatccggc agataacggt cgttcatgcc
    19201 ctcgggctca tcggtgtgcg cgtagcggta gttcgcgccg tagccctcct ctttcatcaa
    19261 tccggttggg gcgtttcgga tgtgcatggg gacttccggc gttacttcgc ggcgcacgtc
    19321 ggcgagcgcg gcgttgatgc cggcgtaggc ggcgttggac ttgggggcgg aggcaaggta
    19381 cgtggtagcc tgggccagcg ggatgcgtcc ctcgggcatc ccgatcatct gcaccgcctg
    19441 cgccgccgcc gtcgcgagcg gcaacccgcg cgggtcggcg ttgccgatgt cctcggaggc
    19501 cagtatgacg agccgccgcg cgatgaacac cgggtcttct ccggcctcga tcatacgcgc
    19561 caggtagtgg agcgccgcgt ccgggtcgcc gccgcgcacg ctctttatga acgcgcttat
    19621 gacgtcgtag tgctcctcgc gcccgtaacg tagcgctctc cgcccgacag cccgctccac
    19681 gtccgcgacc tcgacgttct tcgtccccgc cgccgcgact tccagagcgt tcagcatccg
    19741 ccgcccatcc ccgcccgaga gacgcagcag gtactctcta gcatcctccg agatccgggc
    19801 tcccagttcc gatagaccgc gttccagcaa cgtacccagg tcttcctccg agagggaccg
    19861 cagccgcaac acccgcgacc gcgacagcag cggcgccgtc acctcgaacg acgggttctc
    19921 ggtagtcgcc ccgataaagt ccacgagccc ctcttcgagc gcgggtagca gcgcgtcctg
    19981 ctgtaccttg ttgaagcggt ggacctcgtc cacgaaaagg agcgtcccgc gtccttcgta
    20041 cttgagacgg tcgcgggcgg cgtccagagc cgcgcgaagg tccttcacac cgctcgtcac
    20101 ggcgcttaaa ggcacgaact cgtcctctac ggtgttcgcc agcacccgtg ccagcgtcgt
    20161 cttgccggtg ccgggcggac cccagaagac tacggagccg atcctgccgc gctcggccac
    20221 cacccgcaga ggtccgttct cgcccgtcag gtgctcctgt cctaccacct cgtccagcgt
    20281 cttgggccgc agcctctcgg cgagcggggc gcggcgggcc aggctctcgg agccggagga
    20341 gtcaaagagg tccatatcgc tagtataccg gccggatgcc cggctcccag cgtgtctcta
    20401 gagcgcctcc ggccgccgct ctccgaaacg ggtggcccgc tcgaaggcgc gggcgagttg
    20461 caggacgccg atctcgtccc gccagcggcc cacgatctgc accccgaccg gcagtccttc
    20521 cggcgtgaac ccgcccggaa cggagaccgc cggcagcccc gtcaccgtga tgtagtagca
    20581 ggacttcatc cagtcgatgt aggtctccat cggctcgccg tctatctcct ccacataggg
    20641 cgtccgcacg ccgaagggcg cgacctgcgt cgtcggcagc accagaaatt cgtagcgctc
    20701 cataaacagc cgcacgcgtt ccaggatctc cgcccgttgt cgctccgccg ccccgatccg
    20761 cggcccggtc aaccgacgcc cctcctcgat attccacacg acggtatcct tcaggagatc
    20821 ccggtgagag tcgagcaggc cgccgtaggc gagctcgaac tgccacgctc gcagcacctt
    20881 gaacacctcg tccgccccag agaagtccgg cgcggcttcg tctactccgc acccgatctc
    20941 ctccatcatc tttccgctcg tctcgaatac ttcccggact tgccgctcga ccggaagccc
    21001 tccgaggtcc gggctccacg cgatccgggt cccccggaag tcccgctccg ggaggggatc
    21061 gaagccgccc cccggctcgg agatggagag tggagcgcgc gggtccggcc cggcaatcgc
    21121 ggagagcatc agggcgacat cctcgactga acggcccatc ggaccctcca cggagagcgg
    21181 cgaccacggc gccatcgagg gccaagatgg gacccggccc ggcgacggcc gcagacccac
    21241 gacgttgcag aacgccgccg ggttcctgag cgacccgccc atgtcggagc cgtcggcgat
    21301 gggcagcata ccgcaggcca gcgcaaccgc cgcgccgcca ctcgaaccgc cgcaggtctt
    21361 cgagaggtcg taggggttcc tcgtcgcgcc gaagacagcg ttgaacgtct gcgaacccgc
    21421 gccgaactcc ggagtgttcg tcttgccgac ggagatcgcg ccggcccgct tcaggcgctc
    21481 cacggtgagg ttgtcgcgct cggggacgtg atccttgtag atcctggagc catacgtcgt
    21541 gcgtattccg gcggtatcgg cgaggtcctt gtgggccacc gggagaccgt gcagcggccc
    21601 cgcctcctcg ccccggtgga gcgcccggtc cgcccgccgc gccccttcga gcgcccgctc
    21661 cggcaggaac gtgacgatgg cattcacggc gggattcgcc tgctctatac gctccaggtg
    21721 cgcccgcatg acttcgaccg ccgaagtctc ccgagaccgg atgagccgcg cgaggtccac
    21781 ggctccaagc agacagaggt ccgtctcagg cccgcttggc atactccact tcccttccgc
    21841 aatgccgacg atctccggcg ctgtatgatt cgaagaggtc cacgcggtca gtatactggc
    21901 gtctcgttcg gtgtttcagg gagggcgatg gacgaagcga ggcacgaggt cttcgagctg
    21961 gcagaccgta tctcggatcg cctcgcggag atcgcgggcg tcgaggcggt ggccctcggc
    22021 ggctcgtggg cgcgcggcga ggcccacccg gactcggacg tggatctcgg catctactac
    22081 cgatccggaa gcccgccccg gattgaggag ttgcggcgcc tggcacggga gctcgacgac
    22141 cgccatccag acgactctct gacggacttc ggcggctggg gccctggatc aacgacggtg
    22201 gatggctcaa gatcgaaggc cgcaacgtgg actggctcta ccgcgacctc gacctcgtcg
    22261 agcgcaccat agaagagtgc cgcgccggca acccatccgt ccactatcaa cccggccacc
    22321 cgcacggctt ccacacgcaa atctacctcg gcgaggtcca ccactgccac ccgctgcacg
    22381 acccgaacta cacgctgcga tccctgaaga acctcgccga cccgtacccc ccgtgcctca
    22441 aacggacaat tgtccagaaa caactctggg aagcgcgctt cgccgttgag acctgccgaa
    22501 agtccgccgc acggggcgac gccttctacg tcgccggctg cctcttccgg tgcgcggcct
    22561 gcctcgcgca gaccctcttc gccctcaacg agcggtacgt tatcaacgag aaaggcgcca
    22621 tcgaagtcgc gggttcgcta gcggtaagtc cgccgtattt tgctgacacc ataggctccg
    22681 tgctcgcgtg tccgggagag agcccggatc tactccagag cagtgtgcga cggctggaag
    22741 agttattaga ggacgtggaa gctctctgtc gggatcagtc ctcggcgtaa cgttcgtcgt
    22801 gctcgcggcg ctggcggtcc atgcggcggg cagcttccaa gacgtcctgg gggtagaggc
    22861 tgcggagggc gccggtctcc aggacttgat caacggagtg ctccgggatc atcatccact
    22921 ccgccgcgtg cggcaccggc aggggcttgt tggccgcccg gtaaaagacc cacactatcc
    22981 agcgcccaag gcgcgattcg gacagaaacc tcaaactctc acctcccgca agccctgatc
    23041 cgggccgaac gatacctcag tcgttatccg tcaaacaaag caaccagatt ctacgatagc
    23101 gtttgtctat tagtagccag gtgaggtgtt cgacataaga aaaccactcg tgccagcggt
    23161 cgaaagacct gtacacctgg gtctcggtgt atcgctcgcc gaaaatagcc ttgcagaacc
    23221 tgtcgcccag ttcctttgct tctgttggcg tacccgggaa ggacatgtgg cagccaccga
    23281 tcaccagttt ttcggcgatc tcaccgtcta gcgtgaacga gctgccccac tcgaattcgg
    23341 gcagcccgaa gaatccgtcc ccgtccagca gcaacccttc ggtccgcaac tccggcagcg
    23401 cgacggctac tgtaggggat acgaagaact gcctaaagaa attcgcctca tctagacggt
    23461 tccgggaagc gaaccagtcg aacactttat gttcggagac gcggaaacat cccagcaata
    23521 ttccaccgtg tatgcgcgtc tcacgcagag ccttcaggca ttcgtctatg gctgaattct
    23581 cgaaaggctc cggatcaaac cgtatgtccc accacatctt ccgcaagtca gtttgcggag
    23641 atgaggtatc gtcccagtct ggttcgagca ttcccatacg atatttcaat gctaacatgc
    23701 cgtgttcagg tcgagccttg caatccggtt accgtcctgc acacgggccg gacaaggacc
    23761 tcaatgcgcc tcctcgtgat acggaacctg cggcaagcaa ttctatccta gccgggctat
    23821 cacggcggcg cacgggtccg acccgttaga atgtggtccg ttcgcacaac gatccgagga
    23881 tgttgggaga ggcatgaaac tagaaggcaa ggtcgcggtg atcacgggcg cggggagcgg
    23941 tatcgggcgc gcgacggcgc tgaagttcgt gcgggagggc gcgcgggtgg tggtcgccga
    24001 gtacaacgag gagggcggcc gggagacggt gcagctctgc gaggaggcgg gcggcgaggc
    24061 gctgttcgtc tacacgaacg tggcggagtt cgagcaggtc gaggcggcgg tggaccgggc
    24121 ggtggaggag tacgggtcgc tcgacgtaat gttcaacaac gccgggaccg gacacttcgc
    24181 gccgctcctg gagcacgacc cgaaggacta cgaccgggtc gtccgggtca accagtacgg
    24241 cgtctactac ggcatcctcg ccgccgggcg caagatggcc gcgctcgggg tgcatggcgt
    24301 gatcgtgaac accgcctccg tcttcgcgtt cctggcctct ccgggcgtct tcggttacca
    24361 cgccgcgaaa ggcgccgtga agatgatgac ccaggcggcg gcgctggagc ttgcgccgca
    24421 cggcatacgg gtcatagccg tcgcgcccgg cagcgtggac acgtccatca tccagggcta
    24481 caaggacatg gggatggagg agcggctcgc ccgcgtccag atgcgcggca agctgttgca
    24541 gccggagcag atcgccaacg ccgtcgccct gctcgtcacc gacgaggccg acgccatcaa
    24601 cggctccgtg gtgatgaccg acgacggtta tgccgcgttc aagtagggcg gtacaatacg
    24661 tccgtgaaca gggagactac tgacttctac gagatggtgg agcgggcgct cgaagggctg
    24721 ccgccggaga tctcggcgtt gctcgacaac gtggcgataa tggtggagga gtggccggag
    24781 ctctcgacgc cgctcgtcgg cccggacgag gacgacacgc tctacggact gtacgagggg
    24841 acgccgctca cggagcgggg ctggggttat tatgcggcgc tgccggacag gatcacgatc
    24901 ttccgcggcc cgctggagcg ggacttccgg ccggaggatc tggaggagca ggtcaggatc
    24961 acggtaatcc acgagatcgc gcaccacttc ggtttcgggg aggaccgcct ggaggagttg
    25021 ggctacggat gaagaagggc cgcatcagca tcgaccagca catagacgcc ccggcggaga
    25081 gcgtcgccac ctacatcggg gacttccgca acgcgaagga gtggatggtc ggcgtggaga
    25141 gcgtcgagaa gatcggcgac gacctgtacc gcctgagcct ggagagcccc atcggcaagc
    25201 tctcgcccga ggcccgcgtc ctggagcaca gcgatcacct gctccggtgg gtctacgcct
    25261 ccactataga cggcggcggc agcgtcgagg tatcgccggg tgaaaacggc acctgcacgg
    25321 tctcgtacag cggcgagttc cggctcaaga ataagctcct gaaccgcgcg gcgcaggcgg
    25381 tcggaatcga gcgcttcgcc cagatgaacg gcgagcggtc cctggcgcgc ctcaagcacc
    25441 tgatggaagc gaggcgctac tgatggggct tttcgagctc agcatcctgc tcttcgtcgt
    25501 cttcctcgtc ctcggaccaa agcgtataac gaacctcctg cgagccctcg gacgcggcgc
    25561 ccgggacttc aagaccgagt tcggaggcaa ggacagagac ccggagctga ccgaagacga
    25621 cgaccgggac gccaacctcc gcaagaagta gccgcggagc aggcggtgtc ccggctttga
    25681 cgccgcttac agtcccagca aaaccctccc gcccggaccc acgcggccgt tcgggggctc
    25741 gcggtccacg ccggagaggt cctcgcccgc gctcttgagg aagatgcgcg gctcctcgac
    25801 atcgagaccg gggttcacgg agtaagtctg ccaggtaatg ccccgccagg tgaatgcgag
    25861 cagtacgggc ggctcctccc ctctatccag cgagacatgc acctccggct ggcccagggt
    25921 gcgcgagagg gcgttcatct ggcggcgctg ctcggagtcg ttgaagaggt ctatcccggc
    25981 gcgcagacgg ttggcgggtt cggcgaggcg gcggcgaagc tcgtactcct cttccaggtc
    26041 cgcgcgaagc tcgtctatcg tggcgcgcag gccggcggcc tcggactcgc gctcctccag
    26101 gtcgcgctcc aagccctcga cgcggcgcag ggcgtcttcc agggcctgat ccttgcgggt
    26161 gatgaggtcc gaggcgtcga ggctgaaggc ttccagctcc tcggcgcggc gctccagcag
    26221 gtagtcgcgg cgggagatct caccgtcctt gcgctctagc tcgcgctgta gccgctctat
    26281 ctggcgctcg taaccctccc ggccccggcg gcgctcgcgg gcctcttcct caagggtacg
    26341 gatgcgctcc ttcaaccgcg cgcgttcgtc ctccagcgcc cggacgcggc cctcgcccgc
    26401 ctgccgggag gcccaggctt catccgcggc gagcctggaa gaggagacct gatcgtacag
    26461 ctcccggatg tggttgtcct tgatcccgag ccgcgccttc aactcgaccg ggtcgtcgcc
    26521 gtactcccgc tgaacctcga cctcggaggt atccgcgccg ccgtccgtcc cggcgtccat
    26581 gccatccggc tcgcgctctc catccacctc tcggcggtcc ggctcgtcca cggctcccta
    26641 ctcctccgcc ggctttccgt ccgtcttctt ccggctctcc ttctcgtcca ccgcggcggc
    26701 ctctccttct acaggctccg ccttcgtaac ttcggcttgc gccggttcag atgccccggt
    26761 ttcggatgcc tcggacctca ccttcgaacc ctcggcggac ccatcatccg ccgtcccgga
    26821 cgcggtagcc gctgccttag cggcttcgga cgtatcccgc ggccgcccgg cgccggcatc
    26881 cgggtccttc gcgccgggtt tcggcgtatc tgccgcttcg gacctgggag cttctccgcc
    26941 ggtcttgaga gacagcccgg cctcgcgagc tgctcgccgt tcggccttta tccgctcccg
    27001 ctcctcgcga gacatcttct taggagcatc atccccgtca ccggcaggtt cagctccggc
    27061 aggttcggca ccagcaggtt cggcaccagc aggttcggct ccagccgccc cggcgccggt
    27121 ctccttcccc ggttgcgggg ctttcagcga ctggcccgat tcgcgggccg cccggcgctc
    27181 ggctttgata cgttcccgct cctcccggga catcttcttc ggggcgtcgc tcgcggcagc
    27241 tccctcagcg ccaccttcgt cagccttctc cggttgcgag gttttcagcg atttcccggc
    27301 ctcgcgggcg gcgcggcgtt cggccttgat cttctcccgc tcctccgccg tgagcttctt
    27361 tggcgccggc ttcgcctccg cttccgctcc ttctgcggca ggctcggcgt ctccggcggt
    27421 cttctcggat ttcggctcct cggcgggctt ggggaggccc gtgatctcgc tgaccctcga
    27481 cagaagctcc tcggctgacg gccgctcatc actccaggcg tgtaccacct taccgtccgg
    27541 gcccacgata aagaccgccg gacgagtgta gtcgccgcgc tccttctcgc gctcaaggag
    27601 cccccattgg cggctcgtga ccctcgcgta atcgtagagg acgtaggact tcaggccgga
    27661 cgcgcgggcg aactcccgag cctcgtccgg ctcggcgacg ccgatagcgg ccacggagcc
    27721 tgcggcgagg ttgatctcat cctccttcgc cgccagttcc ctgaagtacg cgacatcctc
    27781 ctcactccac gggcggtaga acgcgaccac caccggcccc cgcacggtac gcatgctcaa
    27841 ccgcaactgc ccgccctggg cgctgggcaa attgaactcc ggggcctccg ccccaacctc
    27901 cgggaccggc atcgtcttcg agcgcgtgaa catcgtcctc ctaatgctta tacgaacgaa
    27961 ccctcttggg aatactacag gctgcgcgtc cgtttggccc gcccgctctg ctatcctccc
    28021 ataccatgcg gctcctgatg acccacggat acatgttctc cggctccggt agcaatgtct
    28081 acgtgcagaa cctctgccgc gccctggtgc gcgagggcca cgacgttcac ctgctctgcc
    28141 aggaacccgc cccgctctca tacgacttcg tagacgaaca ccggacggca aacgctgacg
    28201 gcgcgcacac gcacggcgag caagagacac cctactccgg ccgctgcatc gtctacaacc
    28261 cggagatagg cgacctcctg ccagtttacg tctacgacga gtacccccgc tggcgcgtca
    28321 agactttcct ggacctctcg gatgaagagt tcgaggacta cgtggagaag aacgccgccg
    28381 ccctccgcgc cgtcctcggc gcgagcggcg ctgaggctgt agtgaccaac cactccgtgc
    28441 ccggaccgct catcgcgcgc cgcgctctcg aagggaccgg cgttccctac accagcatcg
    28501 tgcatggtag ctgcctgcag tacgtctcgc gacggagcga gacgtacatg ggcgtcaccc
    28561 gcgagggtct cgaaggagcg aggaaaatac tggccctctc ctcccacagc gcgggcacca
    28621 tcgccgaaga cttcccggag atgaaagaga agaccctcgc cctccccggc ggcgtggata
    28681 ccggcctctt ccgcccggac gccctcgacc ccggcgccct gaaagcgctc atcggcggtc
    28741 ccggacgcgg gccggatcag cgcgcggccc tgttacatgc actggatgac aggacgagca
    28801 gcgccgcaga gttggcgaag cggctacata aggtagaagc gtcctacgac ggccgctcgc
    28861 acgactcgga tgcgggggaa cgtttgggaa ggttgctcga agacggctct cctatcatca
    28921 tctacgtcgg caagctcata cactcgaagg gcgtccacag cctcatctcc gccttcgccc
    28981 gcatccaccg cgagaccgga gcgcatctcc tggtggtcgg ctacggcacc ttcagggagg
    29041 ggctggaagc tctcgttcac gccctcggca cgggcgaccg cgagaccctg gagacccttg
    29101 ccagaggcgg cgcgctcttc gagggcggtc ccgagaagcg catggagcac ttcgaggtcc
    29161 cggagagcct gctgcgcgag gcgtccggga tggaggactc ggtccagttc gtcgggccgc
    29221 tataccacgc ggaactggcg aagctcttac cggcggcgga ggtggcggtc gtgccgtcca
    29281 tcttcccgga gaccttcggg ctcgtagcgg ccgagttcgc ggcgagcggg gtggcgccgt
    29341 tcgtggcgga ccactccggg ctgcgggagg cgggcggcat cgtcgggcgc ggcctcccgt
    29401 tcgacctgcg ggtcgggatg gatcgtttcg aggaggacct cgcccgcgct ctacgagact
    29461 acctggagct cccggaagag gagcggcggc ggtgccggga ggtcgtgcgc cgcaacagcg
    29521 tggactacct gagctggaac acgctcgcgg agaaggtagt aaaaatcgcg ggcgaaaaag
    29581 actaaggctt ggagatagag tacatactca gggacttcgg tctcatcctg ggcgcggggc
    29641 tagtctccca gctaatcgcc aggatcatcc ggttgcccga gatgatcgtg ctggtggcga
    29701 tgggagccct gatcggcccg tccatactcg gccttgtcgc gaaccctctc ggtagcgtcg
    29761 gttcgcaact cctcttcaac atcggggtcg ccttgatcct gttccacggc ggcgtcggta
    29821 tctcgctgcg cgtcatctcg aagaccgcgg tcgggcttgg cctgctcgtc ctgccgggtg
    29881 tgttgctcac cgccctgatc gtggccctcg ctgtcgctcc ggtgttcggc gtcgctctcc
    29941 cgatcgcgtt aatggtcggc gccgtgctcg cggcgacgga ccccgccatc ctcatccccc
    30001 tgttcgagcg catcaagctg cgccccaaag tctcccagac ggtcatcgcc gagtcggcgt
    30061 tcaacgaccc ggtcggcacc gtcctgacgc tgacggtggc gggagtggtc agttccggtc
    30121 acttcacggt ctcgggaccg gcgctggatt tcgggcggga gctcgttctg ggtaccctga
    30181 tcggcgtggg agccggcctg atcctcgccg tcctagtcgc ttcgtcggcg cgaataggga
    30241 tctgggacga ggcgcccggc gtcgccatcc tcgcggtggt ggcgcttgtg tacttctcca
    30301 acgaggcgct ccacggctcc ggctacctcg ccgccttcgt gatgggtctg atcgtcggca
    30361 atatggacga gttcaagctg ggccagagcg aggagcacgg aaaactcctc gaagatttcg
    30421 cggcgcagac cgcgcagatc gccacgctgc tcgtcttcgt taccctgggc ctgaacctgc
    30481 ctttcgacgc cctgggcaaa tacttcttcg gcggcctcat cataatggcg gtcttcatct
    30541 tcgtggcccg ccctgtcgcc gtcctggcat gcctgctccc ggaccggcgc gggcagtgga
    30601 cgtggaagga gatcatcttc ctcagttggt gccgcgagac gggagtcgtc cccgccgcgg
    30661 tcggcgcgct gcttttggcc gacaaagtcc caggcgcgca actcgccgtc tcgatggtcg
    30721 ccctcgccgt gtgcgcaacc ttgctcctcc aggctactac cgccgggttc gtcgcaagta
    30781 agctcggact gatcgaagac ggtaattcag atacctccgg ttgaccatct cccgccaaac
    30841 gtccgaaaga cggtacaatg aactccgggc aagcgcgata gagagggagc gatgatccag
    30901 ctcaccgaca gtttcaaccg aaagatggac tatcttcgca tctcggtcac ggaccgttgc
    30961 aatttccgct gccagtactg catgcccgag gacatccagt tccaggacaa gtcccacatc
    31021 ctcacgttag aggagatgct caccttcgcc gaagcctgcc tccaactcgg cgtaacgaag
    31081 gtccgcgtaa ccggcggtga gccgctagtc cgccgcggcg tcgtctggtt cgtcagccag
    31141 ctaaaggcaa tgggcttcga ggacgtgacc atgacgacca acggtttcct cctcaaggag
    31201 aacctcgaag gtctcgtgga ggccggccta gaccgcatca acatctctct cgacaccctg
    31261 cagccggaga agttcgcctt catcacccgc cgcaaccact tcgagagagt gtgggaggct
    31321 gtaatggcgg ctctggagac gccgctctcg ccggtgaagc tcaacgccgt cgccatgcgc
    31381 ggggtgaacg acgatgagat ctccgacatg gcgaagctga cgatgaagta ccccatgcac
    31441 gtccgcttca tcgagctgat gccccttaac ggcgacaccg acggctcgcg cttccgcaag
    31501 ctgttcatct ctggcaagga gatcctggag aaggcccagg gagagttggg cgacctgcgt
    31561 cccatagaga aagaagaccc cgcagccccc gccgacgagt tcaagttcga gggcgcgccc
    31621 ggaacgttcg gggtaataac gcccgtctcg gagccgttct gcgcgaggtg cagccgtctc
    31681 aggctgacgg cggacggcaa gatccacccc tgcctgctca cggacctgga cgtgccggtc
    31741 aaggacgcca tccgtagcgc cgaccccatc cccgccatcc acgaggtgct ctggcacacc
    31801 gccggagtaa agcccgcagc cggcctcacc ctcccagagg aggcgcgcaa ccgcaccatg
    31861 agccagatcg gcgggtaacc tacccggagt cgttgctgga tccctccaaa ccgtagtccg
    31921 gcagcggtcc tccggcgtcc tccaagacgt agtccttgaa catccgcttc actatctcgg
    31981 ggtgtttgcc ggcgatatct ctgtccatgc cgggatcgct ctggatatcg aagagcttcg
    32041 cgcccgcgcc gcttgtctgt gagatcaggg catagcggtc gtcgcgggcc caggaatggt
    32101 cgtgataccc gagggtgaag tgcggcctcg cgttcatctc atccccctcg aacagggcgt
    32161 agaggttctg gccgtctatg ggaccggatg gtctcatgcc aagcgccccg agcaccgttg
    32221 gagcgacatc gtgcgtggaa gcgaagtagt cggtggtctc gccggccttc ttgccctgcg
    32281 gatgccttat gaggaagggc acgtcggtga gctcggggta gagcgcctcc gaaaccttcc
    32341 cggcgaaccc gtgctccccg aaggctacgc cgtgatccga cagcaggatg agcagcgtgt
    32401 tctctcccag gttcagttcg tggaacttct ccatgaagct gccgagccag tggtccacca
    32461 tcgacacttc cgccgagtag agagtatgca tcctccggag cgccttctcg ccgagccagg
    32521 cggaacgtcc gctcttcgac gacatcagct tggggccgtc gtgatcgtcg tagagcctga
    32581 tgtagttctc gggcgggtcc cacggctcgt gcgggtcgta agcgtccacg agcaagaaaa
    32641 atggaccttc tgtctgcgcg gctgcactca ggaagtccat ggcggtgatg aagacctgcg
    32701 gcgcgaacca gtcttcctcg cggatacgcc ccctcgtatt ggcgaaatgc tggcgaacga
    32761 tgtccgtagc ttcctgcctg actccgccgc cttccaggac cctgtccagt tccgtctccg
    32821 agatggctga ggccggccgg aagaagtccc tctcctgccc ccggatgaag tggaaggctt
    32881 taaagcccct gtgcatgtcg tagaacggct tgaactggtg aaccgtgtcg gtgacgaaca
    32941 tggtgtcgta gccggaatcc gagaggacct cagccagcgt cgtctggtct tcggggatag
    33001 gctgccagcc gggcatcttg aagaggtccg acttcaccgg cttccagtcc ctgaaaggcc
    33061 aggtcctcct gcccgtgtga atggcccggc gcgcgcatat ggtcggcaga gactcaggat
    33121 acgccctcgt aaacatcagg ctctccctgc taagcgcgtc caggtttgga gtctggatcc
    33181 aacggttccc ataagccccg acgtggtcct tgcgcaggct gtctatcacc acgactacga
    33241 cgttcatctc ggtatcccca ccccacaggt tgctacatcc cgaactgccg gcggcggtcc
    33301 caagaacggc ggctcctgct atcccggccc cactcagcct cataaaatcc cggcgtgtca
    33361 gcgtatgtct cacctacatc accacgaaaa gatccaccct tataccggcc cctgaagact
    33421 ataataaact aatcgcccga cggcgcagag gagaggagtg taaaaccctc gctccgccgc
    33481 ccccatcccc gccatccatg aggagaaccg caaccgcgct ataagcccgt tcggcggttg
    33541 acgtgcgtgt cgagtacgat caccgcgcct cggtgcgagg ctttccagtc attcccagcc
    33601 acgctttgaa aaggctcatc gtacttcacc agctcgccac tcagaggatt agaacatccg
    33661 gtctttcgcc gacagcatca cctcaggcct cccccgtgcg tagaggcaga ccatctatcg
    33721 taacggtccg atcttccttg acgaggacat cggtcttgtc tggctccaca gtcactcgcc
    33781 tctctgctta ctatctggcg atcagtccgg ttctatggtc gcgatcaagc cctccgcccg
    33841 gagcccttcc tcgtacattt ccgcgagctc tttgtggcac ttggcagcta cggctttgcc
    33901 gttggtgtgg gcctccagca taatcgagac ggcgcggcgc aggctcatgc gcgggatgac
    33961 cttccggagc acctgtatga cgtgttccat cggcgtgtag tcgtcgttgt gcaggatgac
    34021 cttgtagggc ggctcggtgg tcatctttcg ccgggtcgtc tct
//
LOCUS       DASSMB010000002         4879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_507318, whole genome shotgun sequence.
ACCESSION   DASSMB010000002 DASSMB010000000
VERSION     DASSMB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4879
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507318"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..231
                     /locus_tag="VFJ72_00185"
     CDS             40..231
                     /locus_tag="VFJ72_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477907.1"
                     /translation="MEIPKAMIVEQVRSRSGAEKAQEADRELPDKVDTDTDAELLSKY
                     DIDPGELQETFKGQSPTAG"
     gene            complement(390..1541)
                     /locus_tag="VFJ72_00190"
     CDS             complement(390..1541)
                     /locus_tag="VFJ72_00190"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7477908.1"
                     /translation="MLPLEGVKVLDLSRVLAGPYATMVLADLGADVLKVEHPERGDDT
                     RHWGPPFAGEGEARESAYFLAVNRNKRSVGVDLKTPEGLELVKKLATDADVVIENWRR
                     GALAKIGLGYEALQESNPDLIYCSITGFGPGPDEDRPGYDFLMQARAGIMGITGQPGG
                     EPMKVGVAAADIFCGMTAANAILAALYRRNVTGEGARLEVPLFESTLSWLANRGQEYL
                     ISGKDTGLIGNAHPSIVPYQTFDASDKPIVVAVGNNTQFAALCRAVGRPDLAEDERYA
                     TNPDRVANREELIEVLQEEFGKRTADEWAEEIRAAGVPCGPVNTLADVFEDEHVLGSG
                     ILREVEHPAAGTLKTIGSPVLLDGERLPIRRPPPTLGQHTDEVEGGDWT"
     gene            complement(1546..1737)
                     /locus_tag="VFJ72_00195"
     CDS             complement(1546..1737)
                     /locus_tag="VFJ72_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477909.1"
                     /translation="MEEPHEVPFDEIEYQVGDGSEPLLASASEALLLPEAVPGAEAQR
                     SLDLSRGPIQLFLVDGTGA"
     gene            complement(2183..3688)
                     /locus_tag="VFJ72_00200"
     CDS             complement(2183..3688)
                     /locus_tag="VFJ72_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7477910.1"
                     /translation="MESRNDQGLRGGALSTGEAMIVSMGFMAPSVAMFFNTPFIAGFA
                     GGAMPLAMLFSFGAVFLVALGLSQLARRTASAGSVYSIVSNAINPKTGFMGMWFFIIG
                     YGIFEAATYSIFASFASEEMQRIFGIAMPWVIPFFVIAGLVYGLSVLGITQSVRGGLI
                     FLAYEILIVTIMLVSVIAQGGAEGNTLAVFSPTTGSGLGGVFLGMIFGIMSFVGWKAA
                     ASLGEETRDPHTSIPRALLGAVAVIGIYYVFVTYAATIGFGPDNMDKFAGDAAWFDTL
                     TRQYMGSGWAPFVGIAALTGAIASAIGIHNSTVRLLYALGRDGVLPRALAKVHPTRRS
                     PWVAASVQAGYSVVLGLIFGYLVFGDPSTTYGYFGGLGTLAVLFVYIFINVSVFLYFR
                     NKERENFSFVRHAIVPLAATAAVGLPIYGLIYPVPDPPYNLWPYLIALWAIIGFAFLF
                     FISRRRPEIVEVMGRAFGESGDDEPDTTSDLPRRTTGRDITGERPRTESAE"
     gene            4005..>4879
                     /locus_tag="VFJ72_00205"
     CDS             4005..>4879
                     /locus_tag="VFJ72_00205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET7477911.1"
                     /translation="MSQMKAIRVEEFGEPEVLRYVDAERPSPGEGEVLIEVRSAGINY
                     ADTMRRRNQYVVSQDLPFVPGSEVAGIVAEVGEGVESVSISDRVVTLLGTGGYAEYAV
                     APARNLIPIPHGLGFDEAAAIPLQGLTAYHCIKTSGALKEGESVLVHAAAGGVGTLAV
                     QMAKLLGAGKVIATASSDEKLDLAKSLGADVLINYTEEGWPEKVREATDGKGADIILE
                     MVGGDFLEKNLKCLNVFGRMVVYGAASGERGSIIPLALMKRCHAVVGFYLPQIMSRPD
                     LFVPSLRETLGWISSG"
BASE COUNT          924 a         1570 c         1546 g          839 t
ORIGIN      
        1 tcgcggcggc gagttcgacc atgaagagaa ggagggaaaa tggagatacc caaggccatg
       61 atcgtcgagc aggtgcgtag ccggagcggc gccgagaaag ctcaagaagc ggacagggaa
      121 ctccccgaca aggtggatac cgacaccgac gccgagttgc tctcgaagta cgatatagac
      181 cccggcgagt tgcaggagac gttcaaggga cagtccccga ccgcgggtta gatcccgtaa
      241 acaaaactag cgccagagag ccgtccgggg aactgtcctg ggcggctctt ctcatgaccc
      301 gcaccgcctt cccgccgccg aaatccgggc tggattcata ctcccgatta cggtccttgg
      361 tgtaggagcg aagggtcgaa aagtctcttc tatgtccagt ctccgccttc cacctcatcc
      421 gtgtgctgac cgagtgtcgg cggagggcgg cgtatcggga gccgctctcc gtcgagcagc
      481 accggcgagc ctatcgtctt cagggttccg gctgccgggt gctccacctc tcgcagtatc
      541 ccggagccga gcacgtgctc gtcctcgaac acgtcggcga gggtgttgac cggaccgcag
      601 ggcacaccgg cagcacggat ctcctccgcc cactcgtccg ctgtccgctt gccaaactct
      661 tcttgcagga cctcgataag ctcttcgcgg ttggcgacgc ggtccgggtt ggtggcgtaa
      721 cgctcgtctt cggcgaggtc cggacgcccg acggcccggc agagcgccgc gaactgggta
      781 ttgttgccga cggcgacgac gatgggtttg tccgaggcgt cgaaggtctg gtaagggacg
      841 atggacgggt gagcgttccc aatgaggcca gtgtccttac cgctgatgag gtactcctgt
      901 ccccggttcg cgagccagga cagggtggac tcgaagagcg ggacttcgag gcgcgcgcct
      961 tctccggtta cgtttctcct gtagagcgcc gccaggatcg cgttagctgc ggtcatcccg
     1021 cagaagatgt ccgccgccgc gacgcccacc ttcatcggct cgccgccagg ttgtcccgtt
     1081 atgcccatga tgcccgcccg cgcctgcatc aagaagtcgt agccgggacg atcctcatcc
     1141 ggccccggac cgaagccggt gatggagcag tagatgaggt ccgggttcga ctcttgcagg
     1201 gcctcgtacc ccaggccgat cttcgccagc gcaccgcgcc gccagttctc tatgaccaca
     1261 tccgcgtccg tggcgagctt ctttaccagc tccagacctt ccggcgtctt caagtccacg
     1321 cctaccgagc gcttgtttcg gttgaccgcc aggaagtagg ccgactcgcg ggcttcgccc
     1381 tctcccgcga agggtggtcc ccagtgccgg gtgtcgtctc cgcgttcggg gtgttcgacc
     1441 ttgaggacgt ccgccccgag gtcggcgagc accatcgtcg cgtagggacc ggcgaggacc
     1501 cgtgagagat cgagaacctt cacgccttcg agtggcaaca tggctctacg ctccagtgcc
     1561 gtctacgagg aagagttgaa tcgggccgcg agacaggtcg agacttcgct gagcctctgc
     1621 gcccgggacg gcctccggca gcaggagagc ctcgctcgcg gaggctagta gaggttcgct
     1681 tccgtcaccg acctgatact cgatctcgtc gaagggaacc tcgtgcggct cttccatgtc
     1741 gtcgatcctc cttcgtcgcc ttgcgagatg ccgtgaacgc gccccgcgct cttcctggag
     1801 ccttcgcgtc acactcatgg ttctccatct gttgagaagg ttaccggatc gccgtcgccg
     1861 ccgcgaaggt catggcccgg ccacggggac gtccgacagc ccgccttccg aaaggttctc
     1921 gatgctccgt acgaatccta cctcggaggt gccggaaatc gggaggcgga gttcgtagcc
     1981 ggtccgggga gaagctcgga tggcagccac cccgccgggt ctacttccac caggttagtc
     2041 ccggcgtgga gggctgtcct gtcctgatcg gcggagcggc gccgcacaac tggtcattct
     2101 gcggcaccgc acgctcataa aggttgctga gagccggagc gccgggctgc tcctgttccg
     2161 ctctaaagcc cgctgcgtcg cgctactccg ccgattccgt gcggggccgc tctccggtaa
     2221 tgtcgcggcc cgtagtccgt cgcggcaggt cgctcgtcgt gtccggctcg tcgtcgcccg
     2281 actcgccaaa agcccgtccc atgacctcga cgatctctgg tctgcggcgg gagatgaaga
     2341 acaagaacgc gaatccgatt atggcccaga gcgcgatcag gtacggccac aggttgtacg
     2401 gcggatcggg gaccgggtag atcaggccgt atatcggcag gcctaccgcc gccgtcgccg
     2461 ccagcgggac gatcgcgtgc ctgacgaacg agaagttctc ccgctctttg ttgcggaagt
     2521 acaggaagac cgagacgttg atgaagatgt acacgaagag taccgccagc gtcccgagac
     2581 ccccgaagta accataggtg gtcgaggggt ccccgaagac gaggtacccg aagatgagac
     2641 ccagcactac cgagtacccg gcctgcaccg atgccgcgac ccacggtgaa cggcgggtgg
     2701 gatgcacttt agcgagtgcg cgcggcagga cgccgtcgcg cccgagagcg tagagcagac
     2761 ggacggtcga gttgtggata ccgatagcag aagctatcgc tccggtgagg gcggcgatgc
     2821 cgacgaacgg cgcccagccc gagcccatgt actggcgcgt cagagtgtcg aaccatgccg
     2881 cgtcgccggc gaacttatcc atgttgtccg gaccgaagcc tatggtcgcg gcgtaagtga
     2941 cgaagacgta gtagatcccg atgacggcga cggcgccgag cagggcgcgc gggatgctcg
     3001 tgtgcgggtc gcgcgtctcc tcgcccagag aagccgccgc cttccagccg acgaaggaca
     3061 tgatcccgaa gatcataccc aggaacacgc cgccgagccc gctgcccgtc gtcgggctga
     3121 acacggccag agtgtttccc tcggcgcctc cctgcgcgat caccgagacc agcatgatcg
     3181 tgactatgag gatttcgtag gccaggaaga tcaaaccgcc gcgcaccgac tgcgtgatgc
     3241 ccaggaccga gagcccgtag accagccccg cgatgacgaa gaacgggatc acccacggca
     3301 tggctatgcc gaagatgcgc tgcatctcct cgctcgcgaa cgaggcgaag atagagtacg
     3361 tcgccgcctc gaagatccca tacccgatga tgaagaacca catacccatg aagccggtct
     3421 tcgggttgat ggcgttcgag actatcgagt acacgctgcc cgccgaggcc gtcctgcggg
     3481 cgagctgcga aagccccagc gccaccaaaa agaccgcccc gaaagagaac aacatcgcca
     3541 gcggcatcgc gccaccggcg aacccggcaa tgaacggtgt gttgaagaac atcgccaccg
     3601 atggcgccat gaaacccatc gagactatca tcgcttcgcc ggtcgagaga gcgccgcctc
     3661 tcaacccctg gtcattcctg gactccaact aagagcctcc tccacatgtc agggctccca
     3721 agggagcccg agataaacaa caccaaaaca aacaacacca aaacgtctcg cgaatattaa
     3781 ccattcttgt agggataacg ctacagcaat aggatcaatt tttccggccg gggcggcaaa
     3841 atctttatac ctacgtccac ctcgcgtttg gcgctcgttt gagaggagag atctctggct
     3901 cgtcaggggc ccccgcaagg cagttgcctg gataagggcg ctgttccgga cttgactggt
     3961 atagtctggc agttgcttac gtggagtcca gtgggaggag cggcatgtcg cagatgaagg
     4021 cgatccgggt ggaggagttc ggagaaccgg aggtgctccg gtacgtggac gccgaacgtc
     4081 cctcgcccgg tgaaggggag gtcttgatcg aggtccgctc cgccggcatc aactacgccg
     4141 acaccatgcg ccgccgcaac cagtacgtcg tcagccagga cctgcccttc gtccccggct
     4201 ccgaggtggc gggcatcgtc gccgaggtgg gcgagggcgt cgagagcgtc tccatcagcg
     4261 accgggtcgt gacgcttctc ggcacgggtg gatacgcgga gtacgccgtc gctcccgcgc
     4321 gcaacctgat ccccattccg cacggcctcg gtttcgacga ggctgccgcc atcccgctcc
     4381 aagggctcac cgcctaccat tgcataaaga cctccggagc cctcaaggaa ggtgagagcg
     4441 tcctggttca cgcagcggcc ggcggggtcg gcaccctcgc cgtccagatg gcgaagctcc
     4501 tcggcgcggg caaggtcatc gctaccgcga gcagcgacga gaagctcgac ctggcgaagt
     4561 ctctcggcgc tgacgtcctc ataaactaca ccgaggaagg ctggcccgag aaggtccgcg
     4621 aggcgacgga cggcaaggga gctgacatca tcctggagat ggtcggcggc gactttctgg
     4681 agaagaacct gaagtgtctc aacgtcttcg gaaggatggt cgtctacggg gcggcaagcg
     4741 gggagcgggg tagtatcatc cccctcgcgc taatgaagcg ctgccacgcg gtcgtcgggt
     4801 tctacctgcc ccagatcatg agccgcccgg acctcttcgt ccccagcctg cgggagactc
     4861 tcggttggat ctcctccgg
//
LOCUS       DASSMB010000003        23066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_381040, whole genome shotgun sequence.
ACCESSION   DASSMB010000003 DASSMB010000000
VERSION     DASSMB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 23066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23066
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381040"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..358)
                     /locus_tag="VFJ72_00210"
     CDS             complement(17..358)
                     /locus_tag="VFJ72_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7477912.1"
                     /translation="MYGIAVGKTGGTDILVGLTGEFDISALEELRGALDAAAYLRGPA
                     VVDLSGITFLDLLCARELAVRSQIHAHKIRLRSPSRPVLDSARACGLEDWLLLAPGTG
                     DREPPVISGAL"
     gene            626..1195
                     /locus_tag="VFJ72_00215"
     CDS             626..1195
                     /locus_tag="VFJ72_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7477913.1"
                     /translation="MEDRDLAARASSGDVEAFGRLVRDHSALVYRVAMRICGEGDAQD
                     ASQEVWVRVWRNINTFRGGSAFSTWLYKVTVNTCLSAREREARREMREYVSELPFLPE
                     PPGGDADPETAALNAERREEIEKALSYVRPDHRAALVLRHMEGLSYAEIAEILDVPDG
                     TAKGWVSRGRAAMLVALSNNGDGPEGGSS"
     gene            1192..1551
                     /locus_tag="VFJ72_00220"
     CDS             1192..1551
                     /locus_tag="VFJ72_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477914.1"
                     /translation="MTVRGEERWEVLESIGAYAAGELAGEEARAVERLIMERPDHRKL
                     AESYARMLVMLSVLGNESVEAPEAIANYAIRRAYLSAFMRQAEDFLGGIGRSYLDAFV
                     YYFGLRPARRESYGGEI"
     gene            1551..2381
                     /locus_tag="VFJ72_00225"
     CDS             1551..2381
                     /locus_tag="VFJ72_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7477915.1"
                     /translation="MPDNTHDLVRVLTEFTPRLVGAVLVLLLALLVALFLQRLVARFL
                     EKLGLDDLFEQTGASNSLMQVGYDAGPSRLLGIVLFWGVMLTGLAAALSVLGLSSLQS
                     TMDQIVNLSGRALVALVILIAGVMFAGWLAEMVAREADRSGLRSSGGFRRAVFITIVS
                     ISALLAAGQLGLETSILLMLAIVVLATIGLVAALAIGQGLALLSSNVAAGRYVQDGTE
                     VGDVISVDGIEGTVEEMGHASITLRSEDGYLYRIPNRTLLESVVRKQERRPEERGPEE
                     "
     gene            2507..5254
                     /locus_tag="VFJ72_00230"
     CDS             2507..5254
                     /locus_tag="VFJ72_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HET7477916.1"
                     /translation="MKKPRNVSPAHDVLGYERQPLDAIFKPDAVAVIGASDRAGSVGR
                     TIMWNLISNPFGGTVFPVNRNRPSVLGIKAYPSVTDIPQRVDLAVIVAPAPAVPEIIR
                     ECAEAEVPGAIIISAGFRETGSGGAELERRVLEEARKGRMRIIGPNCLGVMSPNTGLN
                     ATFAGAMARPGTVGFLSQSGALLTAILDRSFKENVGFSNIVSVGSMMDVGWGDLIYYL
                     GDDPKTKSIVIYMESVGDARSFLSAAREVALTKPIIVIKAGRTEAAGKAAASHTGSLT
                     GSDEVLEAAFRRSGVLRVNEIAELFDMAEVLGKQPRPKGPRLTILTNAGGPAVLATDA
                     LITGGGELAGISPETMSSLNEFLPAPWSHGNPIDVLGDADPERYAKTLETAAGDPESD
                     GLLVVLTPQDMTDPTETAQALVPYANSTRKPVLASWMGGSAVSAGTSILNGAGVPTFD
                     YPDTAARAFTNMWRYTYTLRGIYETPDLAADEDDVDRERAGEVMAGVRDAGRTILTEY
                     EAKQVLAAYGIPTVETEVARTADEAAELAERFGYPVVLKLDSETITHKTDVGGVRLNL
                     SGAEEVRQAYTEMQQSITEDFGPEDFAGATVQPMVSLDGYELIVGSSVDPQFGPVLLF
                     GSGGQLVEVYRDRALALPPLTTTLARRMMERTRVFEALKGVRGRAPVETGALEKLLVR
                     FSRLVAEQPWISELDINPLLASPERIIALDARVVLHDPETAEEKLPRPAIRPYPTQYV
                     WTEEARDGAPVVVRPIRPEDEPLMVRFHESLSEQSVYMRYFHMMNLDQRTAHDRLTRI
                     CFIDYDREMALVAERTDPESGEKEIMGVGRLSKGGGVTDEAEFSVLINDRYQRQGLGT
                     ILLSHLLDVGRAEGLKRITAEILFENYPMQRISRKLGFHLHRDTEEMVMKADMDLLQP
                     V"
     gene            complement(5314..5616)
                     /locus_tag="VFJ72_00235"
     CDS             complement(5314..5616)
                     /locus_tag="VFJ72_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477917.1"
                     /translation="MRKLMVLVAMLAMMMVAAAPAFAQATGGDVDLSFVDASQSATVD
                     VTQSQFGDATATSGDVGSAADASIDNSLDVSVTQVNGGFVDDGMFMDDDFGFILIF"
     gene            5922..6179
                     /locus_tag="VFJ72_00240"
     CDS             5922..6179
                     /locus_tag="VFJ72_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7477918.1"
                     /translation="MSILTWIVFGLIAGIIAKLLTPGRDPGGCIITMLLGVGGAFVGG
                     FLYSQLTNRPFNAQFDLGSLIVAILGAVVLLIIYRLLAGRA"
     gene            6367..6891
                     /locus_tag="VFJ72_00245"
     CDS             6367..6891
                     /locus_tag="VFJ72_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET7477919.1"
                     /translation="MAALMERLGLGGREMTDSGHSKNFVLKVVQPSLVGLMDGSVSTL
                     APLFATAFATRDPRVTFLVGLAAAVGAAISMAFSEGLSDDGTLTGRGHPFLRGTITGV
                     ATFLGGILHTLPFLLPNVSMALYVAFGVVGVELLAISFIRYRYFQMSFLMSAMQVILG
                     GALVFSAGILIGSA"
     gene            complement(6904..8130)
                     /locus_tag="VFJ72_00250"
     CDS             complement(6904..8130)
                     /locus_tag="VFJ72_00250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET7477920.1"
                     /translation="MTIPARQLAVVFVSLIAAAALLAFTGAAWSEPPGDLVDEGPGGA
                     EYAAGELIVTYEDQEHEQEVESLGEEAGATVAEDLPDADAKVLEFPEVKSERSQEVRE
                     RDLERIKEKLEKSPGVESVYYNYVRTGMYTPNDPRFRYQYGLRKPGFEKAWSRTRGRG
                     TRVAVIDSGAAIQHPDLQGRVVAQYDFVNNNSTVEDLHGHGTHTAGTVAARTGNGIGV
                     AGGCPGCRLIIGKALNRDLVGNDSDIANAITWSVDHGAKVVNLSLGGPGEKSILKGAI
                     DYATRKGANVVAAAGNGGTGPIYPAAYANVIAVAATGPNDGRDSSFSTGSYIDVAAPG
                     INILSTVPGGYEYRSGTSMSSPHVAALAGLLRGQGRGPLATRYRILRTAVDLGPRGKD
                     PYYGYGRINADRATRR"
     gene            complement(8207..8563)
                     /locus_tag="VFJ72_00255"
     CDS             complement(8207..8563)
                     /locus_tag="VFJ72_00255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7477921.1"
                     /translation="MVTGVAVVWMPVQDIERAKGFYGDTLGLTVSKEDGDWAEVNANG
                     LTIGLNGREPEGAQSDGGPVVTFQPEGSLEDTVSGLKEKGVEFPAEISEHPWGRVATF
                     KDSEGNDIQLYEPPKS"
     gene            complement(8659..9321)
                     /gene="lipB"
                     /locus_tag="VFJ72_00260"
     CDS             complement(8659..9321)
                     /gene="lipB"
                     /locus_tag="VFJ72_00260"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET7477922.1"
                     /translation="METERRASSSESIEVRRLPGLTPYAEGWEMQAELVRLRKRGGIP
                     DTLVLLEHPPVYTLGRAAKDASNLGAGEDYLRSLGAEVFWIDRGGDVTFHGPGQVVGY
                     PILRLKVRDTHAYLRELEEVVIRALADYGLEGWRHPAYTGVWVGENKICAIGVKFSSG
                     WITSHGFALNVKTDLSWFDRVTPCGIREHGVTSLQRELNRDVTLAGVEDRITLHFLES
                     FG"
     gene            complement(9331..10248)
                     /gene="lipA"
                     /locus_tag="VFJ72_00265"
     CDS             complement(9331..10248)
                     /gene="lipA"
                     /locus_tag="VFJ72_00265"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HET7477923.1"
                     /translation="MPFRQQSRGVPGSHGRPDWLKARIPDGETYRDIKETMRGLSLHT
                     VCEEARCPNIGECWNNRTATFMILGNVCTRSCGFCAVLTGKPTELDIDEPHRVADAAK
                     QMGLKHAVITSVNRDELKDGGAGIFAATIQAIRQQVPGCAVEVLTPDFKGDRDAIQTV
                     IEARPDTFNHNIETVPRLYPAVRPQAKFRRSLEVLRYAKELNPDGLTKSGFMVGLGEV
                     EEELHQTMRDLRDHDVDILTIGQYLRPSENHLPMSRYYTPREFAELKKYGVSLGFKHV
                     ESGPLVRSSYHAHEQTTDARIAASAGASV"
     gene            complement(10422..10883)
                     /locus_tag="VFJ72_00270"
     CDS             complement(10422..10883)
                     /locus_tag="VFJ72_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7477924.1"
                     /translation="MGGEAGYRWAETLEEILSVFREAFEMDVAFISEFVGEDQVFRVL
                     EGERETFGIEEGTSLPLEGSICQRVVDGRVPNAIPDTRADERVKDLDITRAADVGSYV
                     GFPLRLSDGRLYGTLCCASHSPDPWLRDRDLRLVRDLADRATARLEREGML"
     gene            complement(11020..12432)
                     /gene="lpdA"
                     /locus_tag="VFJ72_00275"
     CDS             complement(11020..12432)
                     /gene="lpdA"
                     /locus_tag="VFJ72_00275"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7477925.1"
                     /translation="MADKFDLVIIGGGNAGYIPAIRASQLGMKVALIEKREGGHLGGT
                     CLNVGCIPTKALLQTAHLLHDAKNGKEFGVKTGNVELDYPTAAKRRESVVNQLRKGVQ
                     GLMKKNKVTVYNGVGSFVEPKKIKVETGDGTEELEAENVLISTGSAVNTLPGLEFDGD
                     RVISSDDIVTNNEDYPDSIIILGSGAVGVEFASMYNDFGTEVTIVELLDKIVPLEDPE
                     ISDALRKEFEGRGMKVLTGVKADPESLEKTDSGVKLKVEGKDGDETLEAECLLVAVGR
                     KTVTEDLNLDATKVEVSDRGIIQVDEYGRTAEDGVYAAGDVIGGYWLAHAAGHEGIIA
                     VEHMAGEDPMPMDQSLVPRVTFCRPEVASFGLTKAQAEEEGYEVKEAKFPFRAIGKAL
                     IEGEPNGFFKIVADSETDLILGMHAIGPKVTDLIAEGVFAKLVEGTPQEIGMAIHAHP
                     SLAEVVSEAAMAVEGHAIHF"
     gene            complement(12451..13311)
                     /gene="mutM"
                     /locus_tag="VFJ72_00280"
     CDS             complement(12451..13311)
                     /gene="mutM"
                     /locus_tag="VFJ72_00280"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET7477926.1"
                     /translation="MPELPEVETIKEDLRELVVGSEIERAGVLDHSLIEQPAEEEFVR
                     RIAGAGISGARRRAKHLIVELSTGDSLVFQLKIGGQLLLVPPVEEPSTALMLMLGLDG
                     GRKLFLRDETGFSRARLLDEQELEERLSALGPEPFGDEFSPEYLRGKLGSRRVQIKPA
                     LLDQKVVSGIGNIYVDEILYDARLHPRRKTNTLAAAEWAALYEAIKKNLAAGIEHRGT
                     TVRLYRDVLDRPGQHQNHLRVFEKHGKPCPGCEGTVVREKVGGRPTHFCPACQPEDGF
                     EGERQLESLW"
     gene            complement(13358..14092)
                     /locus_tag="VFJ72_00285"
     CDS             complement(13358..14092)
                     /locus_tag="VFJ72_00285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7477927.1"
                     /translation="MTGWYREDLAHIHDAGFGDYALGSAPGILKILGRSRVREGLVVD
                     LGCGSGLWARELVDAGYRVLGIDISEAMVEISRRRVPEAEFRVGSLFDAGIPPCNAVT
                     AVGEVLNYLFDPQGDEDTLLPLFRRVHGALAPGGVFVFDVAEPGQVRRGISERSFTEG
                     DGWVVLVEKEEDTERSILTRRISTFRQVGESYRRDDETHRQRLYRAPEVAGELRRAGF
                     RVRARRAYGAYRLPEAHAAFVARRPA"
     gene            complement(14105..14836)
                     /locus_tag="VFJ72_00290"
     CDS             complement(14105..14836)
                     /locus_tag="VFJ72_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET7477928.1"
                     /translation="MDSNEFLDRLKREVIGHPALTHPFLERFGDGDASPDGVREFAIQ
                     YYRHVRVSRLYLASLISGCREDEKLQLALAEILFDEYGHLNPDETHPALYRRFLRALD
                     ISEEEWEAPKTLPEIEMYITTHYALTRDPDIRLGLGALGPASEWPVPPIYVRLSEGLR
                     KAAGLPDEALEIFTSHVTMDVTHARIMMDSIAPYAEDEEGQRKVREGAMRSLDARSVM
                     LDGLYKTVYREPVPLAGTSVRVAGE"
     gene            complement(14901..16544)
                     /locus_tag="VFJ72_00295"
     CDS             complement(14901..16544)
                     /locus_tag="VFJ72_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET7477929.1"
                     /translation="MISIKRSPDSSAFQRAQLEQLIRLITPVAFGFALVSEVAAALFR
                     DVYMGFTGAFIFLFGCLVLVARALVRRGRWQSAVNTICAGILGTTLAVALVQPDWFPT
                     LVITSLLTVMVALPYTEDRVLLPLLVGAWMVALVVAVVSRLIPYSSELPYVVQSLFLV
                     GSLAATVATLLLLLWQFRSRLTRTLAQTRAAEERYALAARATNDGLWDWDLTTDEVYF
                     SPRWKEMLGYGEGEVGTGSSEWFDRIHPDDRAGVEERLRAHLNGDKGNFATEYRALHR
                     DGEYLWMLARGVAVWDEEGKAVRIAGSQSNINERKRAEEELKKRAAQLASYNDELEQF
                     AFSVSHDLRAPLRWITGFSKILIEDQAEKLDAESKDYLYRILEASDSMGERVDSLLEI
                     SRLTRRKLHREPVDLGLMAQGIADDLRKSHPESNVEFIIAEGLSADVDEQLMRVAIEN
                     LLDNAWKFTRDQSSPRIEFGAVDEEDERVYFVKDNGVGFDETYSENLFGPFQRLHTDE
                     EFEGTGLGLATVQRIIHRHGGRVWAEGEIGKGAAFFFTL"
     gene            complement(16541..17428)
                     /locus_tag="VFJ72_00300"
     CDS             complement(16541..17428)
                     /locus_tag="VFJ72_00300"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477930.1"
                     /translation="MSSFGTFKDEADYRRFHATRIGRLAARFHPDASLRVLQLVSNWY
                     GWMFLRDDQRDESELGERPEQLAASNARFLEILEGSKPVSEEVPLAHSLWDLRRRVLT
                     EAPAREWMEQLIASIGEHFDSTVWEATNRSRGVIPDMDNYIRMRPVTGGLNVDTQFIE
                     IAEHTHLSPEVRNHPILQTLTRTSNNAVCWANDIFSLHKEIERDVHNLVLVLQRAEGL
                     TLQQAIDRAAEMHNAEVRTFIDSAAQLRSLGEQPDENLERFVGVLQTRMRGFVDWANE
                     SGRYQPSVGVSQSEPGDRT"
     gene            17871..18371
                     /locus_tag="VFJ72_00305"
     CDS             17871..18371
                     /locus_tag="VFJ72_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477931.1"
                     /translation="MSVPRQREEERLDPHVYRRGINGLTVEEGLARAFPRVMEILARS
                     GERYSEFERQLVRQTMRACLISQVSRANLYRIAGILVADRRDEGAHLGSSLALAYVAA
                     LREAFLAGLSVGERVETPLGSGEVCYVSADGADAVVMLDAGRRSEVWLPVEKLSRPGG
                     PLRRAS"
     gene            18443..19363
                     /locus_tag="VFJ72_00310"
     CDS             18443..19363
                     /locus_tag="VFJ72_00310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET7477932.1"
                     /translation="MEKTRARVICNPTSGGGAYDPDGIREALEGLDIEWIETEGPDDA
                     VEAAREYRDGLLLVAGGDGTINDVVNGIGQAGFPESVTLGLLPSGTGNDLAATLCIPE
                     DPEQAVKVIRQNLARTLDVAQVQSEGVGERFFINVATGGLGAEISDANDEELKEKWGK
                     LSYLRASLEVSRDFDVRELDLYLDGELRKVRAVNVVVGNCRYAGGGWLAAPKANPEDG
                     LLDVVIIETLGVTDLLTLAPASLARSSYLDKEGVFFARAKEIRVETQPPGLEFTADGE
                     VIGDEPAEFTLIPHALNVIVGPEYTPEPAE"
     gene            19373..19615
                     /locus_tag="VFJ72_00315"
     CDS             19373..19615
                     /locus_tag="VFJ72_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET7477933.1"
                     /translation="MVKVLLFGAAADAAGTRQLELRLDNAGTLHDLWPLLESQHPGLS
                     PMRDTLAFAVNGEYARMDDRVSPGDEVAVLPPVSGG"
     gene            19627..20043
                     /locus_tag="VFJ72_00320"
     CDS             19627..20043
                     /locus_tag="VFJ72_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET7477934.1"
                     /translation="MVEEPLNVGALVSAAQRPDCGAVSTFVGTTRVDESSGASVEYLE
                     YEAYRPMADRKLEEIGAEILDRWDVRHVSIVHRLGRVDPGEASVAIVVASPRRGPAFE
                     ASRYAIERIKEIVPIWKREVWSDGYVWVGSQVGTRT"
     gene            complement(20080..20460)
                     /locus_tag="VFJ72_00325"
     CDS             complement(20080..20460)
                     /locus_tag="VFJ72_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HET7477935.1"
                     /translation="MSGEMPHDSPSRDQTVVREHLANERTLLAWVRTGVGLISIGLVV
                     ERAGALAAAAPAKVGPISASEVFGLALAVLGAITLVIGTVQFLRNRRRISSGDFAPAV
                     AAYLVVVAGSLALAGAFIAYVLLQ"
     gene            complement(20457..21410)
                     /locus_tag="VFJ72_00330"
     CDS             complement(20457..21410)
                     /locus_tag="VFJ72_00330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7477936.1"
                     /translation="MDVTVIGCGVIGLTTAIRLREAGMDASIVAAALPPDTTSSVAAA
                     IWYPYKAYPEDRVLAWGKRTFQEFVDLSDLPQSGVRIREGVEVYRRPVPDPWWKEAVP
                     GVRRCREDELPPGCRDGHVFIVPIIEMPVYLGYLMQRFADAGGTVEQRAVSSLEEAGD
                     GVVVNCSGLGAREIAGDEGMSPIRGQVVRVRNPGIPRFILDEDNAEGVTYVVPRSEDC
                     VLGGTADEGRWDTEPDIKTAGNILRRCARLEPLLEGAEVLEHKVGLRPGRMEVRLETE
                     ILSGGTTCVHNYGHGGSGVTLSWGCAGEAVGLVREAFGVAV"
     gene            21460..21945
                     /locus_tag="VFJ72_00335"
     CDS             21460..21945
                     /locus_tag="VFJ72_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7477937.1"
                     /translation="MVKERAQGEMDFGIRVAAVIAREGSLLLVRHQKPDRPPYWVLPG
                     GRLEPGESIPECAVREVLEETGLDASFSGVLYVSEFMREGRHTVDITVRMTAEGEAQL
                     GGDPEVVPGSEPTLSELRWVALEELNELDLLPARLKERLLRDAAGGWPEREAYLGGDR
                     G"
     gene            21929..22660
                     /locus_tag="VFJ72_00340"
     CDS             21929..22660
                     /locus_tag="VFJ72_00340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7477938.1"
                     /translation="MVIGVSDPQRVLELVVLQNAILASLTIGLFWLGVFVARRFGRSS
                     SYSLSPLGFSRPRAGVFAGAGIGLLVGIGALMISIPLNLASAAVLERFGYSTQSTVQG
                     PFMEGLREWVQQSPILAIPAIVLVVVILGPAVEELTFRGGVFNALYHLGRLASKGNEN
                     KRFVSTVEKVSFAAAALVSSAVFALLHLEPVLLPALLILAVVLCVLFRRTGSLLPCFV
                     AHATFNSFATLLIILSGLGVFQVPV"
BASE COUNT         3913 a         7785 c         7195 g         4173 t
ORIGIN      
        1 gctccgggtc cgcaggctag agagcgccgg agatgacggg aggctcgcgg tcgccggtcc
       61 caggggcgag gagcagccag tcttccaggc cgcaggcgcg ggcggagtcc aggaccgggc
      121 gggaggggga gcgcagcctg atcttgtggg cgtggatctg ggagcggacg gcgagctcgc
      181 gggcgcacag gaggtccagg aaggtgatgc cggagaggtc cacgacggcg ggcccccgga
      241 ggtaggccgc ggcgtcgagc gcgccgcgca gttcttcgag cgcggagatg tcgaactctc
      301 ctgttagccc tacgagaatg tctgtaccgc cggtcttgcc aactgctata ccgtacaaga
      361 tcacaccttc cccaggatac cgatggttgc tctcaacaca cctgcttgat tatttagacc
      421 accgcggcgg ccggatggtt acactttgcg gcgcgaaaga gaagaaattc tgtcccgcgg
      481 ccccgttagc ggagcgcgcc gccgcgagcc gccgggttct gttatcttgt ggcatggaga
      541 tgcaccggcg cggcgactca tcggttccat cagttttccg ggttccccta caaggttccc
      601 aagcggagtg ccgctcttga cgtacatgga agacagggac ctggccgcgc gcgcctcctc
      661 gggagatgtc gaggcgttcg gcaggcttgt ccgggatcac tccgcgctcg tataccgggt
      721 ggcgatgcgg atatgcgggg agggtgacgc tcaggacgcg agccaggagg tttgggttcg
      781 tgtctggagg aacataaaca ctttccgagg tggtagtgct tttagtacgt ggctatacaa
      841 ggttacagtc aacacctgtc tcagcgcccg cgagcgggag gcccggcgcg agatgcgcga
      901 atatgtaagc gagcttccgt tcctgcccga gccgcccgga ggagacgcag atccggagac
      961 cgcggcgctc aacgccgagc ggcgcgaaga gatagagaaa gctctctcct acgtgcggcc
     1021 cgaccaccgg gccgctctcg tgttgcggca catggagggg ctctcctacg cggagatagc
     1081 ggagatcctg gacgtccccg acggcacggc gaagggctgg gtatcccgcg gtcgggcggc
     1141 catgctcgta gctctctcga ataacggcga cggcccggag ggaggctcct cgtgacggtg
     1201 cgcggcgagg agcgctggga ggtgctggag agcatcgggg cgtacgccgc cggagagctg
     1261 gcaggcgagg aggcgcgcgc ggtcgagcgg ctcataatgg agcgcccgga ccaccgcaag
     1321 ctcgccgagt cctacgcccg catgctcgtg atgctctccg tcctgggcaa cgagagcgtc
     1381 gaggcgccgg aggccatcgc gaactacgcc atccgccggg cctacctctc ggcgttcatg
     1441 cggcaggccg aggacttcct aggcggcatc ggacgctcat acctcgacgc tttcgtctat
     1501 tatttcgggt tgcgtcccgc gaggcgcgag agctacggag gagagatcta atgcccgaca
     1561 acacacacga ccttgtgcgc gtcctgaccg agttcacccc ccggctcgtt ggcgcggtgc
     1621 tggtgctcct gctggcgctc ctggtcgcgc tcttcctgca gcgcctggtg gcccgcttcc
     1681 tggagaagct ggggctggac gacctcttcg agcagaccgg agcctccaac tcgctcatgc
     1741 aggtcggcta cgacgccggt ccgtcgcgcc tgctgggcat cgttctcttc tggggcgtca
     1801 tgctcaccgg cctcgccgcc gctctcagcg tgctcggcct ctcctccctg cagtctacga
     1861 tggaccagat cgttaacctc tccggccggg cgctggtcgc gctcgtcatc ctcatagccg
     1921 gcgtcatgtt cgccggatgg ctcgcggaga tggtcgcccg cgaggccgac aggtccgggc
     1981 tgcgcagctc gggcggcttc cgccgggccg tctttataac catagtctcc atctccgcgc
     2041 tgctggccgc cggtcagctc ggccttgaga cttccatact cctcatgctc gccatcgtcg
     2101 tgctcgccac catcgggctc gtcgccgcgc tcgccatcgg gcagggtctc gccctgctct
     2161 caagcaacgt cgccgccggc cgctacgtgc aggacggcac cgaggtgggc gacgtcatct
     2221 ccgtggacgg catcgagggc accgtcgagg agatgggcca cgcatccatc acgctccgct
     2281 ccgaggatgg ctacctctac cgcatcccca accgcaccct cctggagagt gtagtccgca
     2341 aacaggagcg ccgtccggag gagcgcggcc ccgaggagta gggcctctcc gtggcctccg
     2401 tagaagagat tacgcgcccg gacggggcgc cgtgttacga cttccggtag acggcgacag
     2461 tccttcgccg agtgcaagag aagaatctcg caagaggagt cagacgatga aaaagcccag
     2521 gaacgtcagc ccggcccacg atgtgctcgg atacgagcgg cagccgctcg acgctatctt
     2581 caagcccgat gcggtggcgg taatcggggc gagcgaccgg gcggggagcg tcgggcgaac
     2641 gattatgtgg aacttgatct ccaacccctt cggcggcacg gtcttcccgg tgaacaggaa
     2701 ccgtccgagc gtgctcggta tcaaggcata tccctccgtc acagatatcc cccagagggt
     2761 agacctcgcc gtcatcgtcg cgcccgcccc cgccgtcccg gagataatcc gcgagtgcgc
     2821 cgaggcggaa gtccccggcg cgatcatcat ctccgccggc ttccgggaga ccggctccgg
     2881 gggcgcggag ctggagcggc gggtgctgga ggaggcgcga aagggccgga tgcggatcat
     2941 cggcccgaac tgcctcggcg tgatgagccc gaacaccggc ctcaacgcca ccttcgcggg
     3001 cgcgatggcc cgacccggca ccgtcgggtt cctcagccag agcggggcgc tcctcacggc
     3061 catcctcgac aggagcttca aggagaacgt cggcttctcc aacatcgtct ccgtcggctc
     3121 catgatggac gttggttggg gagacctgat ctactacctc ggcgacgacc cgaagacgaa
     3181 gagcatcgtc atctacatgg agtccgtcgg cgacgcccgc tcgttcctgt cggcggcgag
     3241 ggaggtagcc ctcacaaagc ccatcattgt cataaaggcc gggcgcaccg aggccgccgg
     3301 aaaggccgcc gcctcccaca ccggctccct gaccggctcc gacgaggtcc tcgaagccgc
     3361 cttccgccgc agcggcgtcc tgcgcgtgaa cgagatagcc gagctgttcg acatggccga
     3421 ggtcctcggc aagcagcccc gcccaaaggg gccgcgcctg accatcctga ccaacgcggg
     3481 cggccccgcc gtcctcgcca ccgacgccct cataaccggc ggcggagagc tggccggcat
     3541 ctcccccgag accatgagct ctctgaacga gtttctgccc gctccctgga gccacggcaa
     3601 ccccatagac gtgctcggcg acgccgaccc ggaacgctac gcgaagacgc tcgaaaccgc
     3661 cgccggagac ccggagagcg atggccttct ggtcgtcctc accccccagg acatgactga
     3721 tcccaccgag accgcccagg ccctcgtccc ctacgcgaac agcacccgca agcccgtctt
     3781 ggcgagctgg atgggcggct cggccgtctc cgccgggacc tccatcctca acggcgccgg
     3841 cgtcccgacc tttgactacc cggacaccgc cgcccgcgcc ttcaccaaca tgtggcgcta
     3901 cacttacacc ctccgcggca tctacgagac cccggacctc gccgccgacg aggacgatgt
     3961 ggaccgcgag cgggccgggg aggtcatggc cggggtgcgc gacgcgggac ggaccatcct
     4021 caccgagtac gaggcaaagc aggtcctcgc cgcctacggc atcccgaccg tggagaccga
     4081 ggtcgcccgt acagccgacg aagccgcaga actggccgag cgcttcggct atcccgtggt
     4141 cctcaagcta gactcggaga ccatcaccca caagaccgac gtcggcggcg tgcgcctgaa
     4201 cctgagcggc gccgaagagg tccggcaagc ctacacggag atgcaacagt ccatcaccga
     4261 ggacttcggg ccggaggact tcgcgggagc gacggtgcag ccgatggtga gcctggacgg
     4321 gtacgagttg atcgtcggga gcagcgtgga cccgcagttc gggccggtcc tgctcttcgg
     4381 ctccggcggc cagctcgtcg aggtctacag ggaccgggcg ctggcgctgc cgccgcttac
     4441 cacgacgctc gcccggcgca tgatggagcg cacccgcgtc ttcgaggcgc tgaaaggcgt
     4501 gcggggccgg gctccggtgg aaactggcgc tctggagaag ctgctcgtgc gcttctcccg
     4561 gctcgtggcg gagcagccct ggatctccga gctggacatc aacccgctcc tcgcctcgcc
     4621 ggagcgcatc atcgcgctgg acgcccgcgt cgtgctgcac gatcccgaga ccgccgagga
     4681 gaaactgccc cggcccgcta tccgccccta cccgacccag tacgtctgga ccgaggaggc
     4741 gcgggacggc gccccggtag tcgtgcggcc gatccgcccc gaagacgagc ccttgatggt
     4801 caggttccac gagtccctct ccgagcagag cgtctacatg cgctacttcc acatgatgaa
     4861 cctcgaccag cgaaccgccc acgaccgtct cacgcgcatc tgcttcatag actacgaccg
     4921 cgaaatggcg ctcgtcgccg agcgcaccga cccggagagt ggagagaaag agatcatggg
     4981 cgtcggacgt ctcagcaagg gcggcggcgt cacggacgag gcggagttct ccgtcctgat
     5041 caacgaccgc taccagcgcc agggcttggg cacgatactc ctctcccacc tcctggacgt
     5101 gggccgcgcc gagggcctca agcgcataac cgcggagatc ctgttcgaga attaccccat
     5161 gcagcgcatc agccggaagc tcggcttcca tctccaccgc gacaccgagg agatggtcat
     5221 gaaggccgac atggacctac tgcaaccagt atagaaacga aaaggagcgg ctccccttgg
     5281 aagggagccg ctcctcgaaa ctggacgctc tgtctagaag atcaggatga agccgaagtc
     5341 gtcgtccatg aacataccgt cgtcgacgaa cccgccgttg acctgcgtga cggatacgtc
     5401 gaggctgttg tcgatggagg cgtccgccgc gctgccgaca tcgccggagg tagcggtggc
     5461 gtcgccaaac tgtgactggg taacatctac cgtcgccgac tgggaagcgt caacgaacga
     5521 gaggtccaca tccccacccg tagcctgggc gaaagccgga gccgccgcca ccatcatcat
     5581 cgccagcatc gccaccagga ccataagttt ccgcatcttc tcttctcctt caattgggtt
     5641 gcgccttgct ctctctaccg tgaactacat gtcgtctaca tgccgcttca cctcttcgtc
     5701 agggtatgtt atctacgaag ttccatacgc ctcggatcac aacctaatcg ccctacggtc
     5761 tctcgctcac ctctcatccg aacacccact acatggtggc accccgcgcg aaattgcgta
     5821 tcacgcaaat gagtgatttt tctctagccc tttcggatta atgaccggcg agaagcggta
     5881 tatggtgaac gtcatacagt cttgaagtgg cggagggaat aatgagcatc ctgacctgga
     5941 tagtcttcgg cctcatcgcc gggatcatcg ccaagctact tacccccggc cgcgaccccg
     6001 gcggctgcat catcacgatg cttctcggcg tcggcggcgc tttcgtcggc gggttcctct
     6061 actcccagct aaccaaccgc ccgttcaacg cccagttcga cctcgggagc ctgatcgtcg
     6121 cgatcctggg cgccgtcgta ctgctgatca tctaccgcct cctcgccggc cgcgcctgat
     6181 ccacacgaga catctccagg cgggaatcgc tcccatctat ggcgcgattc ccgctttttc
     6241 accctcccca caccctggca atcgctaatg actcccatta accagtaata agaactgttc
     6301 ttgatttatg cggtttctcc gctagaataa aggggtgtat gggtcatcta aacggaggat
     6361 ggattcatgg cggcattaat ggagcggctg ggtctcggag gccgggagat gacggattcg
     6421 gggcacagca agaacttcgt gttgaaggtg gtgcagccga gtctggtcgg gttgatggac
     6481 gggtcggtct ccacgctggc gccgttgttt gcgacggcgt tcgccacgag ggacccgcgg
     6541 gtgacgtttc tggtggggct cgcggcggcg gttggggcgg cgatcagcat ggccttctcc
     6601 gaggggctct cggacgacgg cacgctcacc gggcggggtc atcccttcct gcgggggacc
     6661 ataacgggcg tcgcgacgtt cctgggaggc atcctgcaca cgctgccgtt cctgctgccg
     6721 aacgtgagta tggctctata cgtggccttc ggggttgtgg gggtggagct gctggcgatc
     6781 tccttcatcc gctaccgcta cttccagatg agcttcctca tgtccgcgat gcaggtcata
     6841 ctgggcggcg cgctcgtctt tagcgccggt atcctgatcg ggagcgcgta gagtagcttc
     6901 ggtctatctc ctggtggccc ggtccgcgtt gatgcggccg tagccgtagt aagggtcctt
     6961 tccgcgaggt ccgaggtcca cggcggtgcg gagtatcctg tatctcgttg ccagagggcc
     7021 ccgtccctgg ccgcgcagga gcccggccag cgccgcgacg tgcggcgagg acatgctcgt
     7081 gccgctcctg tactcgtagc cgccggggac agtggagagt atattgatgc cgggagccgc
     7141 cacgtcgatg tagctgccgg tgctaaatga gctatctcgt ccgtcattgg ggccggtggc
     7201 agccacggcg atcacgttag cgtaagcggc gggatagatc ggtcccgtac cgccgttgcc
     7261 cgcggcggct acgacgtttg cgcctttcct ggtggcatag tctatggcgc ccttcaggat
     7321 gctcttctca cccggtccgc caagagacag gttgaccact ttggccccgt ggtcgacgct
     7381 ccacgttata gcgttagcta tatcggaatc gttgcctacg agatctcgat tcagagcctt
     7441 gccgattatg agccggcagc cggggcaacc tccggccact ccgatcccgt ttcccgtccg
     7501 ggcggccacc gttcccgccg tgtgagtacc gtgcccgtgc agatcctcga ccgtagagtt
     7561 gttgttcacg aagtcatatt gtgcgacgac ccgaccttgt agatccggat gttggatggc
     7621 cgctccgctg tcgattacgg ctaccctcgt acccctgcct ctggtccggc tccaggcctt
     7681 ctcgaagccc ggctttctca gaccatactg gtatctaaac ctgggatcat tcggcgtgta
     7741 catgcccgtg cggacgtagt tgtaatagac gctctcgaca cctggactct tctcgagctt
     7801 ctccttgatc cgctccaggt cccgctcccg gacctcctgg gaacgctcgc tcttcacctc
     7861 cgggaactcc aggaccttcg cgtcggcatc ggggagatct tctgcgaccg tcgctccggc
     7921 ttcctcaccg agcgactcca cttcctgctc atgttcctgg tcttcgtagg tgacgatcaa
     7981 ctctccggcg gcgtactcgg cgccgcccgg cccctcatcc acgaggtcgc cgggaggttc
     8041 gctccacgca gcgccagtga atgccagcaa tgctgccgca gctattagag atacgaaaac
     8101 aacggcaagc tgccgcgcgg gaatcgtcat gccccctcca agtgtcgcgc ctcgccccat
     8161 actacaggaa acgaggcgcg agatgccagt cgtcaggcgg cggtgtctag ctcttcggcg
     8221 gctcgtagag ttggatgtcg ttgccctcgg agtccttgaa tgtcgctacc cggccccagg
     8281 ggtgctcgga gatctccgcc gggaactcga cgcccttctc cttcaggccg ctcacggtat
     8341 cctccagact tccctcgggc tggaaggtca cgacgggacc gccgtcgctc tgagcgccct
     8401 cgggctcccg gccgttcagc ccgatggtca ggccgttggc attcacctcg gcccagtcgc
     8461 catcttcctt gctgaccgtc aggcccaggg tatctccgta gaagcccttg gcacgctcta
     8521 tgtcttgtac gggcatccag actaccgcta cgccagtgac catgtttcct ccttcggttg
     8581 cgtacacgcg cgaacgtgga agccgatcgc gcttacagca caggattcca gctaataccc
     8641 gcggaaactg taagtaaact aaccgaagga ctccagaaag tgcaacgtta tcctatcttc
     8701 gacgccggcc agggtaacgt cgcggttcag ttccctctgc aggctggtga cgccgtgctc
     8761 gcggatgcca caaggagtta cccggtcgaa ccaggagagg tcggtcttca cgttcagagc
     8821 gaagccgtgg gaggtgatcc agccggacga gaacttgacg ccgatggcgc agatcttgtt
     8881 ttcccccacc cacacgcccg tgtacgcagg gtgccgccac ccctccagcc cgtagtcagc
     8941 gagcgcccgg atgacgacct cctccagctc gcgcaggtac gcgtgcgtgt cgcggacctt
     9001 cagccgcagg atggggtaac cgacaacctg tccgggaccg tggaacgtga cgtcgccgcc
     9061 gcggtcgatc cagaacactt ccgcgcccag cgagcgcagg taatcctccc ccgcccccag
     9121 gttgctcgcg tccttcgccg cccgtccgag cgtgtagacc ggcggatgct ccagaagcac
     9181 gagggtgtcc gggatcccac cccgcttcct aagccggacc aactccgcct gcatctccca
     9241 gccctcggcg tacggcgtca accccggcag ccgccgcacc tcgatagatt ctgaggacga
     9301 ggctctccgc tcggtctcca aacccgcgtc ctagacgctc gcgccggcgc tggccgcgat
     9361 gcgcgcgtcg gtggtctgct cgtgggcgtg gtaagagcta cgcaccagcg gtccgctctc
     9421 gacgtgcttg aagcccaagg agacaccgta cttcttgagt tccgcgaact ccctcggcgt
     9481 gtagtagcgg ctcatcggca gatggttctc cgagggccgc aggtactgcc cgatggtcag
     9541 gatgtccacg tcgtgatctc gcaggtcgcg catcgtctgg tggagctcct cctccacctc
     9601 gccgagaccc accatgaacc cactcttcgt gagcccgtcg gggttcaact ccttcgcgta
     9661 gcgcagcact tcgagcgaac gccggaactt ggcctgcgga cgcaccgccg ggtacaggcg
     9721 cggcacggtc tcgatattgt ggttgaaggt gtcgggccgg gcttcaatga cggtttgtat
     9781 ggcgtcgcgg tcgcccttga agtccggggt gaggacctcg acggcgcagc cggggacctg
     9841 ctgccttatc gcctggatcg tggcggcgaa gatgcccgcg ccgccgtcct tcaactcgtc
     9901 gcggttgacg ctcgttatga cggcgtgctt gagtcccatc tgcttcgcgg cgtcggcgac
     9961 gcggtgcggc tcgtcgatgt ccagctctgt cggcttgccg gtaaggacgg cgcagaagcc
    10021 gcaagagcgg gtgcagacgt tgcccaggat catgaaggtc gcggtgcggt tgttccagca
    10081 ctcgccgatg ttcgggcagc gcgcctcctc acagacggtg tgaagagaga gaccgcgcat
    10141 ggtctccttg atgtcgcggt atgtctcgcc gtccgggatg cgggccttca gccagtccgg
    10201 gcgaccgtga gacccaggaa ctccccgcga ctgctggcgg aagggcaggt actccgtcgg
    10261 gacgccgtcg ggcggcgggg cgacgggctt atcttcccat cggtgtcgga cctcgaacgt
    10321 cttcgtaccg ttttcgagga ctttcaggtt gatctgtctg cgggccacgg ctctcctatc
    10381 tacctcgcgc tctgcggttc atagtatcgc acggcgggcg gttacagcat cccctcgcgc
    10441 tccagccgcg cggtggctct atccgccagg tccctcacga gccgcaggtc gcggtcgcgg
    10501 agccaggggt cgggcgagtg gctcgcgcag cagagcgtcc cgtacagccg tccatccgag
    10561 agccgcagcg ggaagcccac gtacgaccca acatccgccg cgcgggttat atccaggtcc
    10621 ttcacccgct cgtcggctcg ggtgtccggg atcgcgttcg ggacgcggcc gtctaccacc
    10681 cgctggcaga tcgaaccttc cagcggcagg ctcgtcccct cctcgatccc gaaggtctcc
    10741 ctctcccctt ccaggacgcg gaagacctga tcctcgccca cgaactcgga gatgaaggct
    10801 acatccatct cgaatgcctc ccggaacacg gagagtatct cttcgagcgt ctcggcccat
    10861 ctataaccag cctcgcctcc cacaggaggt cctgtttctc cgccactcat tgtgtcgtcc
    10921 tgacacttcc caaacgttcg acaactgacg gacagagatt atagcgctcc ccggcggccc
    10981 gccgtgtatc cggttacggc gggccgccgg gcgagagaac tagaagtgta tggcgtgacc
    11041 ttcgacggcc atggccgcct cgctgacgac ctccgccagg gacgggtggg cgtggatcgc
    11101 catcccgatc tcctgcggcg tcccctcgac cagcttggcg aagacgcctt ccgcgatcag
    11161 gtccgtcacc ttcggcccta tggcgtgcat acccaggatc aaatccgtct ccgaatcggc
    11221 cacgatcttg aagaagccgt tcggctctcc ctcgatgagc gccttgccga tggcccggaa
    11281 cgggaacttg gcctccttga cctcgtagcc ttcctcctcg gcctgggcct tggtgagccc
    11341 gaaggaggcg acctcggggc ggcagaaggt taccctggga acgaggctct gatccatggg
    11401 catcgggtcc tcgccagcca tgtgctctac cgcgatgatg ccctcgtgcc cggcggcgtg
    11461 ggcgagccag tagccgccga tcacgtcccc ggcggcataa acgccgtcct cggcggtccg
    11521 gccatactcg tccacctgga tgatgccgcg gtcgctcact tctaccttcg tcgcgtcgag
    11581 attcaggtcc tcggtgacgg tcttgcgacc gaccgcgacg agcaggcact cggcttcgag
    11641 cgtctcatcg ccatccttgc cctcgacctt gagcttcacg cccgagtcgg tcttctccag
    11701 gctctccggg tcggccttga cgccggtcag gaccttcatg ccgcgtccct cgaactcctt
    11761 cctcagagcg tcggagatct ccgggtcttc gagcggcact atcttgtcga gaagctcgac
    11821 tatggtcact tcggtgccga agtcgttgta catgctggcg aactctactc cgacagcgcc
    11881 gctaccgagg atgatgatcg agtccgggta gtcctcgttg ttggttacga tgtcgtccga
    11941 ggagatgacc ctgtcgccgt cgaactccag tcccggcaga gtattgacgg cggagccggt
    12001 cgagatgagg acgttctcgg cctccagctc ctcggtgccg tcgccggtct caacctttat
    12061 cttcttcggc tcgacgaagc tgccaacgcc gttgtagacg gtgaccttgt tcttcttcat
    12121 caggccctgc acgcccttgc gaagctggtt cacgaccgac tcgcggcgct tggcggcggt
    12181 cggataatcc aactcgacgt tgcccgtctt gacgccgaac tccttgccat ttttggcgtc
    12241 gtggagcagg tgcgccgtct ggaggagagc cttggtcggg atgcatccta cgttcaggca
    12301 tgtgccgccg aggtgaccgc cttcgcgctt ctctatgagt gccaccttca tgcctagctg
    12361 actcgcacgt atcgcgggta tgtatcctgc gtttcctccg cctatgatca cgaggtcgaa
    12421 cttatccgcc accctgttct cctttcacgt ttaccacagt gattctaact gcctctctcc
    12481 ctcgaagcca tcttccggct gacaggccgg acagaagtgg gttggcctcc ctcccacctt
    12541 ttcccgaacg actgttccct cacaccccgg acagggcttt ccatgcttct cgaagacccg
    12601 caggtggttc tggtgctgtc cgggccggtc cagtacgtcc cggtagagcc ggaccgtggt
    12661 gccccggtgc tctattccgg ccgcgaggtt cttctttatg gcctcgtaga gcgctgccca
    12721 ctccgcggcc gcgagcgtgt tcgtcttgcg ccggggatgt agcctggcgt cgtacaggat
    12781 ctcgtccacg tagatgttcc ctatgccgga cacgactttc tggtccagta gagccggttt
    12841 tatctggacc ctccgggagc cgagcttccc ccggagatac tcagggctga attcgtcccc
    12901 gaacggctcc ggtcccagcg ccgagagacg ctcctccagc tcctgctcgt ccagcaggcg
    12961 cgcccggctg aaacccgtct cgtcccgcag gaacagcttc cggccgccat cgagaccgag
    13021 catcagcatg agcgccgtgg acggctcctc gaccggcggt acgagcagta actgaccccc
    13081 gatctttagc tgaaacacca gcgagtctcc ggtgctcaac tccacgatga ggtgcttcgc
    13141 ccgccgccga gcgccggaga tcccggctcc cgcgatccgc cggacgaact cctcctccgc
    13201 gggctgctcg atgagggaat gatcaagcac cccggcccgc tcgatctccg agcccacgac
    13261 cagttcgcgc aaatcctctt tgatggtctc gacctcggga agctccggca tgcgcgtatt
    13321 tataccccct ccgcgtcccg gagccgtggg agacggttca cgccggtctg cgcgcgacga
    13381 aggccgcgtg agcctctgga aggcgatacg ctccgtaagc gcgcctcgcc cggacccgga
    13441 agccggcccg ccggagctcc ccggcgacct ccggcgctct gtataaacgc tggcggtgcg
    13501 tctcgtcatc ccgccggtag gattcgccca cctgccggaa ggtggagatc ctgcgggtca
    13561 ggatgctccg ctccgtatct tcctctttct cgacgagcac cacccagccg tccccctcgg
    13621 tgaagcttct ctccgagatc ccccgccgga cctgtccggg ttccgcgacg tcgaagacga
    13681 agaccccgcc gggagcgagc gcgccgtgta cccggcggaa gagcgggagc agcgtgtcct
    13741 catcgccctg gggatcgaac aggtaattga gcacctcccc gaccgccgta acggcgttgc
    13801 acggtgggat cccggcgtcg aagagcgacc cgacccggaa ctcggcctcc gggacgcgcc
    13861 tccgcgagat ctcgaccatc gcctcggaga tgtctatgcc gaggacacgg tatccggcgt
    13921 caaccagctc ccgcgcccac agaccgctgc cgcagcccag gtccaccacc agcccctccc
    13981 ggacgcggct acggcccagt atcttcagga tgcccggagc cgacccgagc gcgtagtccc
    14041 cgaaaccggc gtcatggatg tgcgccaggt cttctctgta ccagcccgtc acgtccggcg
    14101 gcgctcactc cccggcgacg cgcaccgaag tccccgccag gggtaccggc tccctgtaga
    14161 cggtcttgta gagcccgtcg agcatgacgg aacgcgcgtc gagcgagcgc atggcgccct
    14221 cccggacctt tcgctgtccc tcttcgtcct ctgcgtaagg ggctatggag tccatcatga
    14281 tgcgggcgtg ggtaacatcc attgtgacgt ggctggtgaa gatctccagc gcttcgtccg
    14341 gcagtcccgc cgccttcctc aaaccctcgg agagtcgaac gtagatcggg ggtaccggcc
    14401 actcgctcgc cggtcccagg gcccccagcc cgagccggat gtccgggtcg cgggtcaggg
    14461 cgtagtgggt ggtgatgtac atctctatct cggggagcgt cttcggcgcc tcccattcct
    14521 cctcggagat gtccagcgcg cggaggaagc ggcggtagag ggctggatgg gtctcgtcgg
    14581 ggttcaggtg accgtactcg tcgaagagga tctcggcgag cgcgagctgc agcttctcgt
    14641 cctcgcggca cccggagatg agggaagcca gatagaggcg gctgacccgc acgtgccggt
    14701 agtactggat cgcgaactcc cgtactccat ccgggctcgc gtcaccgtcc ccgaaacgct
    14761 ccaggaacgg gtgcgtcagc gcgggatgcc cgatgacctc ccgcttcaac cgatccagaa
    14821 actcgttgga atccatgcgc gccctttcac cggaagctct ttgcaaaaag tttaccgcct
    14881 gtcggcgagg acgttggaga ctaaagggta aagaaaaacg ccgcgccctt cccgatctcg
    14941 ccttccgccc acacgcgccc gccatgccgg tggatgatgc gctgcacagt tgcgaggccg
    15001 aggccggtac cctcgaactc ctcgtccgta tgcagccgct ggaaggggcc gaagaggttc
    15061 tccgagtaag tctcgtcgaa ccccacgccg ttgtccttca cgaagtacac tctctcgtct
    15121 tcctcatcca ctgccccgaa ctctatcctg gggctgctct ggtctctggt gaacttccag
    15181 gcgttgtcca gcaggttctc tatggctacg cgcatgagct gctcgtccac gtcggccgac
    15241 agcccctcgg cgatgatgaa ctccacgttg ctctcgggat ggctcttgcg gaggtcgtcc
    15301 gcgatgccct gggccataag gccgaggtcc accggttcgc ggtgcagctt cctgcgcgtc
    15361 agacgcgata tctcaagcag gctatccacc cgctcgccca tgctgtcgct cgcctccagg
    15421 atgcggtaca gatagtcctt gctctccgcg tcaagcttct ctgcctggtc ttcgatcagt
    15481 atcttcgaaa agcccgtgat ccaacgcagc ggagcccgca ggtcgtgcga gacggagaac
    15541 gcgaactgct ccagctcgtc gttgtagctc gcgagctgcg cggcgcgctt cttcaactcc
    15601 tcctccgccc gcttgcgttc gttgatgttc gactgagacc cggctatgcg gacagccttc
    15661 ccctcctcgt cccacacagc cacgccgcgg gcgagcatcc acaggtactc gccgtctctg
    15721 tgcagcgccc ggtactcggt cgcgaagttg cctttatctc cgttcaggtg cgcccgcagc
    15781 cgctcctcga cgccagcgcg gtcgtcgggg tgtatgcggt cgaaccactc cgaagagccg
    15841 gtccccacct ctccttcgcc gtacccgagc atctccttcc agcgcggcga gaagtacacc
    15901 tcatccgtgg tcaggtccca gtcccacagc ccgtcgttag tggcgcgggc ggccagcgca
    15961 taacgttcct cggccgcgcg ggtctgggcc agcgtccggg tcagccggct cctgaactgc
    16021 cacaggagca gcagcaaggt ggcgacggta gccgcgagcg agcctaccag aaagaggctc
    16081 tgcaccacgt aagggagttc ggaggagtac ggtatcagcc ggctgacgac ggccaccacc
    16141 agcgccacca tccaggctcc gacgagcagg ggtagcagga cgcggtcttc cgtatatgga
    16201 agcgcaacca tgacggtcag tagcgacgta atgaccagcg tggggaacca gtccggctgt
    16261 acgagagcga ccgccagggt cgtcccgagg atgccagcac agatcgtatt gaccgccgac
    16321 tgccaccggc cccgccgcac cagagcgcgc gctaccagta ccagacatcc gaataagaat
    16381 ataaacgctc ccgtgaaacc catgtagaca tcgcggaaca atgcggcggc gacctccgac
    16441 acgagagcga aaccgaacgc caccggcgtg atcaggcgga ttagctgctc cagttgggct
    16501 ctctggaagg ctgacgagtc tggcgagcgc ttgatgctta tcatgtccgg tctccgggct
    16561 cgctttggga cacgcctacc gacggctgat agcgccccga ctcattggcc caatccacga
    16621 acccgcgcat ccgcgtctgg aggacgccga cgaagcgctc cagattctcg tccggctgtt
    16681 ctcccagaga cctaagctgc gccgcggaat ctatgaaggt acggacttcc gcgttgtgca
    16741 tctccgcggc ccggtctata gcctgctgca acgtcagacc ttccgcgcgc tggaggacca
    16801 ggaccaggtt gtgtacgtcg cgctcgatct ccttgtggag agagaatatg tcgttggccc
    16861 agcacaccgc attgttcgag gtgcgcgtga gcgtctggag gatcgggtga ttccggacct
    16921 ccggcgacag atgggtgtgc tcggcgatct cgatgaactg cgtgtccacg ttcagcccac
    16981 ccgttacggg ccgcatcctt atgtagttat ccatgtccgg gataacgcct ctcgaacggt
    17041 tggtcgcctc ccacacggtc gaatcgaagt gctccccgat gctggcgatc agctgctcca
    17101 tccattccct ggcgggcgcc tccgtcagca cgcgccgccg gaggtcccac agagagtgcg
    17161 ccagaggaac ctcctccgag accggcttgg agccctccag gatctccagg aacctggcgt
    17221 tcgacgccgc cagctgctct ggccgctccc cgagctcaga ctcatccctc tggtcgtccc
    17281 tgaggaacat ccacccgtac cagttggaga cgagctgcaa caccctcaac gaggcatcgg
    17341 gatggaaccg ggccgcgagc ctgccgatcc tcgtggcatg gaacctccgg taatccgcct
    17401 cgtccttgaa ggtaccgaag ctactcatcc actcgacggt cccctcatgc acctcgtcgg
    17461 cgtgcgggtt tatcctcggc gggaaaggcg agtaaagttc agggagaata atcaaatcga
    17521 tttagctttc taacctcatc ccccggctgc aacgagcgga gatagcgcgc tttatgacaa
    17581 tgcgaccgtc cgcgaaccgt caagctatac catgaatcat atagtaatcg caaacgccgt
    17641 gaatcggaca agcgaacacg gcggccgatt ctatttaccc ggctctacgg tcccatccaa
    17701 ggagatcacg aacctcgcaa cggcgcgcca gtgttcccgt aaagactcgc ccggacgatt
    17761 caatcaagca taaaactccc cttttgcagg caatttatag atgataaacg tacgcggcgc
    17821 ggtataattc gcatacgatg gatacgagaa aagagatacg gcaggattgg ttgagcgtcc
    17881 cgcggcagcg ggaggaggag cggttggacc ctcacgtgta ccggcgtggg atcaacggcc
    17941 tgacggtaga ggaggggctc gcgcgcgcct tcccgagggt catggagata ctggcgcgca
    18001 gcggggagcg ttactcggag ttcgagcggc agctcgttcg gcagacgatg agggcgtgtc
    18061 tgatctcgca ggtcagccgg gcgaacctct accgcatcgc gggcatcctc gtcgccgacc
    18121 ggcgggacga gggcgcgcac ctcggcagct cgctggcgct cgcctacgtg gcggctctga
    18181 gggaggcgtt tctggcgggc ctttcggtcg gtgaacgggt cgagacgcca ctgggaagcg
    18241 gtgaggtctg ctacgtctcc gcggacgggg cggacgcggt cgtgatgctc gacgcggggc
    18301 gccgctccga ggtgtggctg ccggtagaga agctctcgcg ccccggcggt cccctgcgcc
    18361 gggcctccta gcatccgcca tcgtagcctg gcggggttag gggtagacta gaagcggtgg
    18421 tataggctac ggagggcggg cgatggagaa gaccagggcg cgggtgatct gcaaccccac
    18481 ctccggcggt ggggcatacg acccggacgg catccgcgaa gcgctcgaag ggttggatat
    18541 cgagtggatc gagaccgaag gacccgatga cgccgtcgag gccgcccggg agtaccgcga
    18601 cgggctcctc ctcgtcgctg gcggcgatgg caccataaac gacgtcgtga acggcatcgg
    18661 ccaggccggc tttccagaga gcgtaaccct cggtctgctc ccctccggca ccggcaatga
    18721 ccttgccgca acactctgca tccccgaaga cccggaacag gccgtaaagg tgatccgcca
    18781 gaacctggcg cggacgctcg atgtcgccca ggtgcagtcc gagggcgtcg gggagcggtt
    18841 cttcatcaac gtggcgacgg gagggctcgg ggcggagatc tccgacgcca acgacgagga
    18901 gttgaaagag aagtggggaa agctctccta cctccgcgcc tcgctggagg tctccaggga
    18961 cttcgacgtg cgcgagctgg acctctacct ggacggcgag cttcggaaag tgcgagccgt
    19021 caacgtggtc gtcgggaact gccggtacgc cggcggcggc tggctcgccg cgccgaaggc
    19081 gaaccctgag gacggtctgc tcgacgtggt catcatagag actctgggcg ttacggacct
    19141 gctcaccctc gcgccggcgt ccctggcgag gtccagctac ctcgacaaag agggcgtatt
    19201 cttcgcgcgg gcgaaggaga tccgggtcga gactcagccg cccggcctgg agttcaccgc
    19261 cgacggcgag gtcatcggcg acgagcccgc ggagttcacc ctgatcccgc acgccctcaa
    19321 cgtcatcgtc gggcccgagt acaccccgga gccggcggag tagaataccg gcatggtaaa
    19381 ggtactgctc ttcggagccg cggccgacgc ggccgggacc cgtcaactcg aactgcggct
    19441 ggacaacgcc gggacgctgc acgacctctg gccgctcctc gaaagtcagc accccggact
    19501 gtcccccatg cgcgataccc tggccttcgc cgtcaacggc gagtacgccc ggatggacga
    19561 cagggtctcg cccggcgacg aggtggcggt cctgccgccg gtctcggggg gctgaccctg
    19621 ttcgccatcg tcgaggagcc gctcaatgtg ggggcgctcg tctccgccgc gcagaggccc
    19681 gactgcggcg cggtctctac ctttgtcggg accacgaggg tagacgagtc atccggcgcg
    19741 agcgtcgagt atctggagta tgaggcgtac cggccgatgg cggaccgcaa gctggaggag
    19801 atcggggcgg agatactgga ccgctgggac gtgcggcacg tctccatcgt tcaccggctc
    19861 ggccgggtgg accccggcga ggctagcgtt gcgatagtcg tggcctcgcc ccggcgcggg
    19921 ccggccttcg aggcgagccg ctacgccatc gagcggatca aggagatcgt cccgatctgg
    19981 aagcgcgagg tctggtcgga cggatatgtc tgggtcggca gccaggtagg cacccgaacc
    20041 tgagagcgcc tgccgtgatc gcccgctgcg acgacacgcc tactgaagca gcacgtacgc
    20101 gataaaggcc ccggcgaggg ccaggctccc ggcgacgacg accaggtacg ccgccacggc
    20161 gggggcgaag tcgcccgagg agatcctgcg ccggttcctg agaaactgca cggtcccgat
    20221 gactagcgtg atcgccccca gcaccgccag cgccagcccg aagacctcgg aggcgctgat
    20281 gggaccgacc ttcgcgggag cggctgcagc cagcgctccg gcccgctcga ccacgagccc
    20341 tatcgagatg agccctaccc ccgtccgaac ccacgcgagc agcgtccgct cgtttgccag
    20401 atgctcccgc accaccgtct ggtcccgcga cggcgagtcg tgcggcatct ctccgctcac
    20461 accgccaccc cgaaagcctc ccgaaccaga ccaaccgcct ccccggcgca accccacgag
    20521 agcgttacgc cagagccgcc gtgaccgtag ttatgaacgc aggtcgtgcc gccggaaagg
    20581 atctccgtct ccagcctcac ctccatccgt cccggacgaa gtcctacctt atgctccagc
    20641 acctccgcgc cttcgagcag cggctccagc ctcgcgcaac ggcgcaggat gttcccggct
    20701 gtctttatgt ccggctccgt atcccatctc ccctcgtccg ccgtaccacc cagtacacag
    20761 tcctccgagc gtgggacgac gtaggtaacg ccttccgcgt tgtcctcatc caggatgaag
    20821 cgcgggatgc cggggttccg cacgcgcacg acctggccgc gtatcggtga cataccctcg
    20881 tcaccagcta tctcccgcgc ccccagtccg gagcagttga cgaccacgcc atccccggcc
    20941 tcttccaagg aggatacagc gcgctgctcc acggtccctc cggcgtcagc gaagcgctgc
    21001 atgaggtagc cgaggtagac cggcatctcg ataatgggga ctatgaagac gtgcccgtcg
    21061 cggcagcccg gcggaagctc gtcctcgcgg catcgccgga ctccgggaac ggcttccttc
    21121 caccacgggt ccgggacggg gcggcggtag acctcgacgc cctcgcgtat gcggacgccg
    21181 ctctgcggaa ggtccgagag gtccacgaac tcctggaagg tacgctttcc ccaggcgagt
    21241 acgcggtcct cggggtaggc cttgtacggg taccagatcg ccgcggcgac gctggaggtg
    21301 gtgtcgggag gcagagcggc ggcgacgatg ctggcgtcca tcccggcctc gcgcaggcgg
    21361 atggccgtcg tcaggccgat cacgccgcat ccgatcaccg taacgtccac cccttcacta
    21421 tagccgaagc acggggcgcg ggatgtatac tccacgcgca tggtcaaaga gcgcgcgcag
    21481 ggcgagatgg acttcgggat tcgggtcgcg gcggtgatcg cgcgcgaagg ctccctgctc
    21541 ctcgtgcgcc accagaaacc agaccgtccg ccctactggg tcctccccgg cggacgcctg
    21601 gaaccgggcg agtccatccc ggagtgcgcc gtccgcgagg tgctggaaga gacgggtctc
    21661 gacgccagct tctccggcgt cctctacgtc agcgagttca tgcgcgaggg ccgccacacg
    21721 gtggacatca cggtccgcat gacggcggaa ggcgaagctc aactgggcgg cgacccggag
    21781 gtagttcccg gttcggagcc gaccctgagc gagttgcgct gggtcgcgct cgaggaacta
    21841 aacgagctgg acctgctccc ggcgcggctg aaggaacgtc tcctgcgcga cgcggcgggc
    21901 gggtggccgg agcgcgaagc gtacctgggt ggtgatcggg gttagcgatc cgcagcgggt
    21961 gctggagctg gtcgtcctac agaacgcgat tctcgcgagc ctgacgattg gtctgttctg
    22021 gctcggggtc ttcgtggcgc ggaggttcgg aaggagttcg agctactccc tctctcccct
    22081 ggggttctca cgcccacgag ccggagtgtt cgccggggct gggatcgggc tgctggtcgg
    22141 aatcggcgcc ctgatgatca gcatccccct gaacctggca agcgcagccg tcctggaacg
    22201 cttcggctac tcgacgcaga gcacggtcca gggaccgttc atggagggac tgagagagtg
    22261 ggtgcaacag agccccattc tcgccatacc ggcgatagtg ctggtcgtcg tgatcctcgg
    22321 ccccgcagtc gaggagttga cgttccgggg cggcgtcttc aacgcgctct accacctcgg
    22381 ccggctggcc tctaaaggga acgagaacaa gcgtttcgtt agcaccgtgg agaaggtctc
    22441 gttcgcagcg gcggcgctcg tctcctccgc cgtgttcgca ttacttcacc tggaaccggt
    22501 gctgctcccc gcgctcctga tcctggcggt agtcctatgc gtcctcttcc ggcgcaccgg
    22561 gagcctactg ccctgctttg tcgcccacgc gacgttcaac tccttcgcca ccctgctcat
    22621 catcctgagc ggtctcggcg tcttccaggt gccggtctag agcaacaaaa acgacccccg
    22681 gatacaatct ccgggggccg cacccttctg agtgctacgc aggtatctgg tctcagcacg
    22741 tccgcctata actactgaag aggcggatgg aaaataaccc agtatcccgc agggttttca
    22801 gcggttctta agtcagagcc acctctccca gaagatacct gcaactacta ctcggtacgt
    22861 ctatctggat cacctcctct ccgtgccttt acgaggggaa gtttactatc ggggtttgta
    22921 tacagcaaga gtaattctta agatttattt tttctcttcg atccgcagcc gcaagaggtc
    22981 cagcgcccgg ttcgcgctgc gctcccggat cgcatccctg gaccgcgccc acgccgacag
    23041 gtccaacctc tcagcctcgg tcttct
//
LOCUS       DASSMB010000004         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1267947, whole genome shotgun sequence.
ACCESSION   DASSMB010000004 DASSMB010000000
VERSION     DASSMB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267947"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VFJ72_00345"
     CDS             <1..678
                     /locus_tag="VFJ72_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7477939.1"
                     /translation="YFEHREQGHDVGNSSFLYPFLGKGAQPLEEVVKEVQGSMLQKMQ
                     EVNRLRAAAAESEAAAISEIAVAVSERLDRGGKIITFGNGGSATDANDLVADCLDPPP
                     GFSSIPAVSLSAESANITAIANDIGAEAVFARQLIAHARPEDVAVGISTSGGSANILA
                     ALAEARKRGLLTVGIVGYDGGRIVGERLADHAVVVRSDYIPRIQEVQASIYHVLRGLI
                     EELRKEG"
     gene            682..1020
                     /gene="hypA"
                     /locus_tag="VFJ72_00350"
     CDS             682..1020
                     /gene="hypA"
                     /locus_tag="VFJ72_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET7477940.1"
                     /translation="MHELSIAESIVRVASNHADGRRVTKVYVKVGHLRQVVPSALAFS
                     FELVAEGTPAEGAELALEEVPIMAACRECGVESQLAAFPLLCNSCGSADLRIVEGEEL
                     LVESLEMEEA"
     gene            1024..1701
                     /gene="hypB"
                     /locus_tag="VFJ72_00355"
     CDS             1024..1701
                     /gene="hypB"
                     /locus_tag="VFJ72_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET7477941.1"
                     /translation="MHRTAVKKVVLEGVLDANDALAQANRDRFDAAGNYVINMMSSPG
                     AGKTALLERTLDRLRGKLRLGILEGDVQTTLDADRLARFHVPLVQVNTDPGFGGECHL
                     DANMVRSGLAELPLDEMDVLIIENVGNLVCPAEFKVGEDARVMVYSVTEGEEKPLKYP
                     LMFRSADLVLVNKVDLLEHLDFDLELFLANLDAVNPGVERILTSARTGQGVEEWCSWL
                     GRRKELP"
     gene            complement(1708..>2219)
                     /locus_tag="VFJ72_00360"
     CDS             complement(1708..>2219)
                     /locus_tag="VFJ72_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7477942.1"
                     /translation="FTGTIYELLSAPVSYIEIVISYVGAAATKSIILGLIILATSGLF
                     VPLEIQHPLWMLAFLILTAVTFSLFGFILGIWADGFEKLQLVPFLIITPLIFLGGSFY
                     SINMLPPFWQTATLFNPVVYLISGFRWSFYGIADVSVGESLAMTVCFMAVCLGTVWWI
                     FKTGYRLKT"
BASE COUNT          426 a          669 c          756 g          368 t
ORIGIN      
        1 tacttcgagc accgcgaaca gggccacgat gtgggcaact cctcgttcct ctaccccttc
       61 ctcggcaagg gcgcgcagcc gctcgaagag gtcgttaaag aggtgcaggg ctcgatgctc
      121 cagaagatgc aggaggtgaa ccgactgcga gcggctgctg ccgagtcgga agccgccgcg
      181 atctcggaga tagccgtggc tgtctcagaa cggttggatc gaggaggcaa gatcatcacc
      241 ttcggtaacg gcggctcggc cacggacgcc aacgatctcg tcgctgactg cttggacccg
      301 ccgccggggt tcagttccat accggccgtc tctctgtcgg cggagtcggc caacataacg
      361 gctatcgcca acgacatcgg ggcggaggca gtcttcgccc gccagctcat cgcacacgcc
      421 cgccccgagg acgtggcagt cggaatctcg acgagcgggg gatccgccaa catcctcgcc
      481 gcgctcgccg aggcccgcaa acgggggctg ttgacggtcg ggatcgtggg ttacgacggc
      541 ggcaggatcg tcggtgagcg gctcgcggac cacgcggtag tcgtccgctc ggactacatc
      601 ccgcgcatcc aggaggtgca ggcgtccatc taccatgtcc tgcgcggcct gatcgaagag
      661 ttgaggaagg agggctgacc cctgcacgag ctctccatag ccgagtccat cgttcgggtt
      721 gccagcaacc acgcggacgg gaggcgcgtc acgaaggtgt acgtgaaggt cggacatctg
      781 cggcaggtcg tcccctcggc cctggctttc agctttgaac tcgtcgccga gggcacgccc
      841 gccgagggcg cggagctggc gttggaggag gtgccgatca tggctgcgtg ccgcgagtgc
      901 ggagtggaga gccagcttgc cgcctttccg ctgctgtgta actcgtgcgg gagcgccgac
      961 ctgcgtatag tggagggcga ggagctactg gtggagtcgc tggagatgga ggaggcatag
     1021 gagatgcacc gcaccgccgt caagaaagtg gtcctcgaag gcgtgctgga cgccaacgac
     1081 gcgctcgccc aggctaatcg cgatcggttc gacgcagccg ggaactacgt cataaacatg
     1141 atgagctctc cgggcgcggg gaagacggcg ctcctggagc ggacgctgga ccggttgcgc
     1201 ggcaagctcc ggctcggtat cctcgaaggc gacgtgcaga ccaccctcga cgccgaccgg
     1261 ctcgctcgtt tccacgtccc gctcgtgcag gtcaacaccg atcccggctt cggtggcgag
     1321 tgtcacctcg acgccaacat ggtgcgttcc ggcctcgccg agcttccgct ggacgagatg
     1381 gatgtcctga tcatcgagaa cgttggcaac ctggtgtgtc cggcggagtt caaggtgggg
     1441 gaggacgcgc gggtcatggt ctactctgtc acggagggcg aggagaagcc gctcaagtac
     1501 ccgctgatgt tccgctcagc ggacctggtg ctcgtgaaca aggtagactt gctggagcat
     1561 ctggacttcg acctggaact gttcctcgcc aacctggacg cggtcaaccc cggcgtcgag
     1621 cgcatcctca cgagcgcccg cacggggcag ggcgtcgagg agtggtgttc ctggctgggg
     1681 cggcgcaagg agctcccgta gcggaggcta cgtcttgagg cggtacccgg ttttgaagat
     1741 ccaccacacc gtccccaggc acacggccat gaagcagacc gtcatggcaa ggctctcgcc
     1801 gacgctcacg tcggcgatcc cgtagaagct ccagcggaag ccgctgatca ggtagacgac
     1861 gggattgaag agggtggccg tctgccagaa cggcggcagc atgttgatgg agtagaagct
     1921 gccgcccagg aagatcaacg gtgtgatgat gaggaacggg acgagctgca gcttctcgaa
     1981 accgtcggcc catatgccga ggataaagcc gaagaggcta aaggtcacgg ccgtgaggat
     2041 taggaacgcg agcatccaca gcgggtgctg gatctccagc ggcacgaaca atcccgaggt
     2101 agccaggatg atcaaaccaa gaatgatcga cttggtcgcc gccgccccga cgtagctgat
     2161 tacgatctcg atgtaggaga caggcgccga caacagctcg tagatcgtgc ccgtgaact
//
LOCUS       DASSMB010000005         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1268053, whole genome shotgun sequence.
ACCESSION   DASSMB010000005 DASSMB010000000
VERSION     DASSMB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268053"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1161)
                     /locus_tag="VFJ72_00365"
     CDS             complement(370..1161)
                     /locus_tag="VFJ72_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477943.1"
                     /translation="MAGSTIAIEDQGTLRKITKIVPYNSGGFAVLAPYHKAQKGYLFK
                     HPVDYSQRRMEISREDNVEYSAEDRVKLSLHPDGFIQFSGENPGKIVSGRDPDTGDPR
                     GLGIMSHPLDTPINTGPTFGCVAWGVADFESLDGIGKSNVIAFAEEDYYYRACTPDTW
                     NGYLIEGFVLEERYWGATRHRGGKLLLTVMPMFEAIGAVFEFRVVPLPGQPLLLGMLV
                     SRVRTEFQSPSGFTLSGPADVREELALAALYPAPQDLEASSLDYR"
     gene            1271..1750
                     /locus_tag="VFJ72_00370"
     CDS             1271..1750
                     /locus_tag="VFJ72_00370"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET7477944.1"
                     /translation="MVRVLFVCMGNICRSPIAQGVFEDRIRRKGLEGEIEVDSAGTHG
                     FYNAGAPPDPRAQRSARARGLDISGQRARLLDAEDCHRFDYILTMDEENYWRVAGLCR
                     GSAVVRPLLDYAPGPETEVPDPYYGGPDGFEYALGLIEEASEGLLREIQARHIGDRV"
     gene            1743..2603
                     /locus_tag="VFJ72_00375"
     CDS             1743..2603
                     /locus_tag="VFJ72_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HET7477945.1"
                     /translation="MSERVISEGVEAHLGERLRSARPLEGGCIGEVYRLELEDGTPLV
                     AKVDREAEANLEREAYMLRYLREKSDLPVPEVYHGSETLLLMQFIEGTNSFSDEAERH
                     AAELVAGLHGITTDSYGHERDTLIGSLNQPNPLTKNWTDFFREERLLYIGRVAYEAGR
                     LPSEDRARVERLAERLEDLISEPNPPALIHGDVWSGNVLAKDGRITAFLDPALYHADP
                     EMELSFISLFNSFGDAFFERYAEIRSIRAEFFETRRDLYSLFPLLVHVYYFGGGYLDS
                     VRSTLSRFGF"
     gene            complement(2623..3093)
                     /locus_tag="VFJ72_00380"
     CDS             complement(2623..3093)
                     /locus_tag="VFJ72_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HET7477946.1"
                     /translation="MSGSIISRTLRLGAIGGLRSMSAPAMVSRAIADGRLDNLEGTPF
                     AMLGSRPVPTILRVLQVGELLGDKLPMTPSRTAALSLLGRMASGAIAGTTLFAAEDRS
                     PAVGGIIGAGAAVAAAFAGEQLRALVGEKTGVPDPVVALVEDGVVLLGGPRLLR"
     gene            complement(3119..4195)
                     /locus_tag="VFJ72_00385"
     CDS             complement(3119..4195)
                     /locus_tag="VFJ72_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET7477947.1"
                     /translation="MDPKTIVVMEGDQTGQELLEESLRVLDPAVTGVDLEFRRFDLSL
                     ENRRATENAVVHEAAAAMKECGLGIKAATITPETSGDVGSPNAILRKGIDGTVIVRTG
                     RRIPGVSPVTGVHAPISVVRMAVDDAYGAEEWREGEGLDEVAYRTEKISRRVCRGVSE
                     FAFIQARKMRAKVFGGPKYTVSPVYEGMLKEEMDRAAENNRDVRYEPQLIDATYALLL
                     TTYGEPLVIPTLNRDGDCISDLVMQMFGSIAGAESLLIALDEDEHPETVMAEAPHGTA
                     PSLQGKDVANPMAMILAGAGLLTFFHEEEASRVSRAIYEATFETILDGVKTADLGGAA
                     GTTEFTDEVIRHVKTKLEVWSALA"
     gene            4357..4680
                     /locus_tag="VFJ72_00390"
     CDS             4357..4680
                     /locus_tag="VFJ72_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477948.1"
                     /translation="MTTGYALWTALSAVPGAGLVLAKDPGSGAYLLLAGLVALAGIVY
                     LAFRRSRKGDGSSGTDGGAGGGIGGSGYYGDFHHGHHASSGDHSGGGSDSGGDGGGGD
                     GGGAD"
     gene            complement(4686..>5258)
                     /locus_tag="VFJ72_00395"
     CDS             complement(4686..>5258)
                     /locus_tag="VFJ72_00395"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HET7477949.1"
                     /translation="EEILEMVLREEVQLGLTRAMSHPEVESLQLYEDELVLVVDPDHR
                     FTARGSASLADVSEETLILFDKDSSYTEQTHALFRNAAIREFRTMEVDNIEAAKRMVE
                     HRLGVAFLPRTAIVRSVSAGNLVLVNVEENPSLNRSIAALKRRDAPASGPVAAFLEVA
                     EKMGEQRSQTHLSPALTAEFENLQLIDLFT"
BASE COUNT          927 a         1731 c         1645 g          955 t
ORIGIN      
        1 gggctgctcg ctcccatagt ccgggtcgaa gcactccagc atcgcctcga tgtcgcggct
       61 attcagcgcc tccagcaact tcccgaccac agtctccatc accgtacctc cacggaccat
      121 tggcccaaaa gcaccttcgc ctgtcggcag gatgccaccg ataccggccc ggcgcacccc
      181 cggcgccgcg tgtccagttg gaacgcggcg aatggatgag agcggaactg ctctacgcgc
      241 ttgtgttcac cctcaccctg gtcatatttc tcaactccat ggagtacctg tacttctccg
      301 tctgacaaca atcagatatg agagatccgc acgcttccaa cggggcctac ggagaccata
      361 acgaaacagc tatcggtagt ctaggctcga agcctcaagg tcttgagggg ctggatacag
      421 cgcggccaaa gcgagttctt ctctcacatc ggcgggaccg ctcaaggtga aaccggaagg
      481 cgactggaac tctgtcctaa cacggcttac caacattcct aacagcaaag gctgacccgg
      541 caacggtacc acccggaact cgaagacggc cccgattgcc tcaaacatcg gcatcacggt
      601 tagcagcagc ttccctccac gatggcgcgt ggctccccag taccgttctt ccaacacgaa
      661 accttcgata aggtagccgt tccaggtatc cggggtgcaa gcccgataat aatagtcttc
      721 ctcagcgaag gcgataacgt tactcttccc gatgccgtcg agggactcaa agtcagcgac
      781 accccacgca acgcagccga aggtcggacc cgtgttgatc ggcgtgtcca acgggtggga
      841 catgatacct agacctcttg gatctccagt atccggatct cgcccggaaa ctatctttcc
      901 aggattctcg cccgagaatt gtatgaatcc atcaggatgc aaggacagct tcacccgatc
      961 ctcagcggaa tactcgacgt tgtcctcacg ggaaatctcc atgcggcgct gactgtagtc
     1021 tacgggatgt ttgaaaaggt agcctttctg ggccttgtgg tacggagcaa gcacagcgaa
     1081 gccaccgcta ttgtagggta cgatcttggt aatctttcta agcgttccct gatcttcgat
     1141 agcgatggtt gacccggcca cgcaacctat tttactagct tacctgtagt ctgtagcaac
     1201 ctcattgtga tagatgtggg cgcggacggt caacctcggg gcgcaataat aactggtaga
     1261 ttaagcagat atggtgcgcg tgttgttcgt ctgcatgggg aacatctgcc ggtctccgat
     1321 agctcagggc gtcttcgagg atcggatacg gcgcaaggga ctcgaaggcg agatagaggt
     1381 tgactcggcc gggacccacg gcttctacaa cgcgggcgct ccgccggacc cgagggcgca
     1441 gaggagcgcg cgggcgagag ggttggatat ctccggccag cgggcgaggc tgctggacgc
     1501 ggaggactgc caccgcttcg actacatcct gacgatggac gaggagaact actggagggt
     1561 ggccgggctg tgccgcggaa gtgcggtggt gcggccgctc ctggactacg cgcccggccc
     1621 ggagaccgag gtgccggacc cgtactacgg cggtcctgac gggttcgagt acgcgctggg
     1681 gcttatagag gaggcttcgg aggggctgct gcgggagata caggcgcggc acatcggaga
     1741 ccgtgtctga gcgcgtgatc tcggagggcg tcgaggccca cctgggcgag cggctgcgga
     1801 gcgcgcgtcc tctagagggc ggctgcatcg gcgaggtcta ccggctggag cttgaggacg
     1861 gcaccccgct cgtcgccaag gtggaccggg aagccgaggc caacctggag cgcgaggcgt
     1921 acatgctccg ctacctgcgc gagaagagcg acctcccggt ccccgaggtc tatcacggct
     1981 ccgagacgct actgctcatg cagttcatcg aagggacaaa cagcttctcc gatgaagccg
     2041 agcgccacgc cgcggaactg gtcgccgggt tgcacggcat aaccaccgat tcctacggtc
     2101 acgagcgcga cacccttatc ggcagcctca accagccgaa cccgcttacg aagaactgga
     2161 cagatttctt ccgtgaggag cggttactgt acatcgggcg ggtcgcttac gaggcgggac
     2221 ggttgccttc cgaggaccgt gccagggtgg agcggctggc ggagaggttg gaggatttga
     2281 tctccgagcc gaacccgccg gccctgatcc acggcgacgt gtggagcggg aacgtgctgg
     2341 cgaaggacgg ccgtattacc gccttcctcg accccgccct ctaccacgcc gacccggaga
     2401 tggagctctc cttcatcagc ctcttcaact ccttcggcga cgctttcttc gagcggtacg
     2461 cagagatacg gtcgatcagg gccgagttct tcgagacccg ccgcgacctt tacagtctct
     2521 tcccgctact ggtccacgtc tactacttcg gcggcggcta cctggattcg gtgcggagca
     2581 ccttgagccg gttcggattt tgagagagct aacgcgacag cgctaacgca ggaggcgcgg
     2641 gcctccaagc aggaccacgc cgtcctcaac cagggcgacc acagggtcgg ggacgccggt
     2701 cttctctccc acgagcgcgc gcaactgctc gccagcaaag gccgccgcca cggcggctcc
     2761 ggccccgatg atgccgccca cggcgggcga gcgatcttcg gcagcgaaca gcgtcgtgcc
     2821 cgcgatggcg ccggacgcca tccggccgag caaggagagc gctgctgtcc ggctcggcgt
     2881 catcggcagc ttgtcgccaa gtaactcacc gacctggagc acccgcaaga tcgtcggcac
     2941 cgggcgcgag ccgagcatcg cgaacggcgt gccttccagg ttgtccaggc ggccgtccgc
     3001 gatggcccgg ctcaccatcg ccggggccga catcgagcgc agcccgccga tagccccgag
     3061 cctcagtgtc ctggaaatga tagatccgct catggttgtc cctctctggt cgctgccgct
     3121 aggccagcgc gctccacact tctagcttgg tcttgacgtg ccttatgacc tcgtcggtga
     3181 actccgtcgt cccggccgct ccgccgaggt ccgccgtctt tacgccgtcg aggatggtct
     3241 cgaaggtggc ctcgtagatg gcgcgcgaga cgcggctcgc ctcctcctcg tggaagaatg
     3301 tgaggagacc ggcaccggcc aggatcatcg ccatcgggtt cgccacgtcc ttgccctgta
     3361 gcgatggcgc ggttccgtgc ggcgcctcgg ccatgacggt ctcggggtgc tcatcttcgt
     3421 cgagcgcgat gagcagcgac tcggccccgg ctatggagcc gaacatctgc atgacgaggt
     3481 cggagatgca gtcgccgtcc cggttgaggg tcgggatgac gagcggctcg ccgtaggtcg
     3541 tcagcagcag ggcgtaggtc gcgtctatta gctggggctc gtagcggacg tcgcggttgt
     3601 tctcggcggc gcggtccatc tcctccttga gcatcccctc gtagacgggg gagactgtgt
     3661 acttcggacc gccgaagacc ttcgcccgca tcttgcgcgc ctggatgaac gcgaactccg
     3721 atacgccgcg gcagacgcgg cgggagatct tctcggtcct gtacgctacc tcatccagcc
     3781 cttcgccctc gcgccactcc tccgcgccgt aggcgtcgtc cacggccatc ctgaccaccg
     3841 agatcggggc gtgcacgccc gtaaccgggc tgacgcccgg tatacgccgt cccgtccgca
     3901 cgatgacggt gccgtctatg cccttgcgca ggatggcgtt cggcgagccc acgtcgccgc
     3961 tcgtctccgg cgtgatggtc gccgccttga tcccgaggcc gcactccttc atcgccgccg
     4021 ccgcctcgtg gaccaccgcg ttctccgtcg cccggcggtt ctccagcgag aggtcgaagc
     4081 gccggaactc caggtccacg cccgtcacgg ccgggtccaa cacgcgcagg gactcctcca
     4141 gcaactcctg gccggtctgg tcgccctcca tcaccactat cgtctttgga tccactaatt
     4201 gcacctctgg ctagtctcgt gtgttcgctc gcctagtata cgactctgca agtgccccat
     4261 tccgtgagat tgtgcccggc tcccaggccg catgtagact atgccttatc aaccgtacag
     4321 agcggggagg agacgcatgg ccggaaagac ctacgcatga ccacaggcta cgcgctttgg
     4381 accgcgctat cggccgtgcc cggcgccgga ctcgtccttg cgaaagaccc gggttcggga
     4441 gcgtacctgc ttctcgcggg cctcgtcgcg ctggccggta tcgtgtacct ggccttccgt
     4501 agatcccgca agggagacgg ttccagcgga actgacggcg gagcgggcgg cggaatcggc
     4561 ggcagcggct actacggcga cttccaccac ggccatcacg cttccagtgg agatcacagc
     4621 ggcggcggga gtgacagcgg cggcgatggc ggcggcggag acggtggcgg ggcagactga
     4681 cctttctacg taaacaggtc tatgagctgc aggttctcga actcggcggt cagggcgggg
     4741 gagaggtggg tctggctgcg ctgctcgccc atcttctccg cgacctccag gaacgcggcc
     4801 acgggaccgg acgcgggcgc gtcccggcgc ttgagggccg cgatggagcg gttcagcgag
     4861 gggttctcct cgacgttcac cagcaccagg ttcccggcgg agacgctgcg cactatcgcc
     4921 gtgcgcggca ggaacgccac ccccagacga tgctcgacca tcctcttggc cgcctcgatg
     4981 ttgtccacct ccatcgtgcg gaactcccgg atggccgcgt tgcggaacag cgcgtgcgtc
     5041 tgctccgtgt agctggagtc cttgtcgaag aggatgagcg tctcctccga cacgtccgcg
     5101 aggctcgccg acccccgcgc cgtgaacctg tggtccgggt ccaccaccag gaccagctcg
     5161 tcctcgtaga gctgcaaact ctccacctcc gggtggctca ttgcccgcgt cagcccgagc
     5221 tgcacctcct cccggagcac catctccagg atctcctc
//
LOCUS       DASSMB010000006        37737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_634630, whole genome shotgun sequence.
ACCESSION   DASSMB010000006 DASSMB010000000
VERSION     DASSMB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 37737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37737
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634630"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFJ72_00400"
     CDS             <1..451
                     /locus_tag="VFJ72_00400"
                     /inference="COORDINATES: protein motif:HMM:NF036577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477950.1"
                     /translation="VRETARLERLSAVVEGLLPEDAELVEARFSGGPLLTLLVDRRDG
                     LVDHEFTTRVVSAISPALEEEGYDGMVEVSSPGIERPLTKPEHYRRFVGHEAKVRVGE
                     PIDGRRNFTGVIDRADDEGFVLQLPEEEVEVELPYRSVTRANLKEDI"
     gene            456..1592
                     /gene="nusA"
                     /locus_tag="VFJ72_00405"
     CDS             456..1592
                     /gene="nusA"
                     /locus_tag="VFJ72_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET7477951.1"
                     /translation="MNTALLSALREIETDKNIPFDTITGVLEESLLAAYEGRESAEEG
                     AEVVLDRETGDLRVMKDGEDITPADFTRIAAQVMRQTFYQRLNEIHNEQLLEEYGSRM
                     GDVVTGIVQQSHRRMTLVDLGRVEALLPASEQVPNERYENGQRIKVYLLEIREPGRGP
                     SMTVSRRHEGLLRGLFELEVPEIYDGYVEIKAVAREAGLRSKVAVYSKESGIDPVGAC
                     VGPRGSRVRAVVSELRNEKIDIIQWDPDPARFIAKALSPARVREVYLDEDERQAEVIV
                     PDDQLSLAIGREGQNARLAVKLTDWKIDIKPASQATEFEDEEEDWEPDEDSAMHRCRA
                     VLSNGRRCANMALPGSLFCGIPSHQAQAAEFEGMVEGTQEVSDE"
     gene            1585..3762
                     /gene="infB"
                     /locus_tag="VFJ72_00410"
     CDS             1585..3762
                     /gene="infB"
                     /locus_tag="VFJ72_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7477952.1"
                     /translation="MSRRVYEIARELGLNNKEVLNRFNAAGIEVKSHSSSVDDSAYEQ
                     VFGNGAGVANGRTEAQEAEAQEARTEAEQSGDGAAVSDAPAEEPVAVPDDRPKKQRGG
                     GKKRRRVVIDASATNRGPRPTAAPAAAKETSRPAAEEKPEISAVRVEPGATVKDLADA
                     LDIAPTQVIKVLMGLGEMKTVTQTLSTEEIELTAEVLGREVEVAAVEEPAPEEVLPED
                     DPGDLVEKPPVVTVMGHVDHGKTSLLDRIRKANVQSGEAGGITQHIGAYQVEAGEGDE
                     RKKITFIDTPGHEAFTEMRARGAKVTDVVVLVVAADDGIMPQTQEAIEHSRAAGVPLV
                     VALNKIDVPNANPDRVLGQLSEQGLMPEVYGGETVTVPVSAKTGEGIDELLENILVVS
                     ELEDLKANPNAPASGYVIEGERDPGRGPVATLLLRRGTLHKGDVVLAGTAYGRVRAML
                     DYTGKRIKEAGPGSPVEILGLSGVPEAGTRFEVVEHERVARDKAQQAEAALRRQELAQ
                     GGSRRTLEELLGEGGVQDLNLVVKADVAGSVEALKEALAKLSTDEVRVNIVRSGVGAL
                     TDSDVMLASASDGILLGFNVRPTNTAKQVAEREGVEIRTYDVIYKALEEIEAAMRGML
                     APEERENETGTAEVRQIFRVPNIGVVAGCYVTSGEIFRTNRVRVIRDGVVIYEGNIAS
                     LRRFKDDARSVRQGFECGIGIENFNDVKEGDELEFFEIVEIPR"
     gene            3785..4054
                     /locus_tag="VFJ72_00415"
     CDS             3785..4054
                     /locus_tag="VFJ72_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET7477953.1"
                     /translation="MRLELELPFASSLKDKRKTIRSLKDRLRRKNVSVVESDNHDSWQ
                     RATMEIAVAAVSRGAAEEKREEIRRSLLNYEELSIVEWREEFVKL"
     gene            4051..4410
                     /gene="rbfA"
                     /locus_tag="VFJ72_00420"
     CDS             4051..4410
                     /gene="rbfA"
                     /locus_tag="VFJ72_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET7477954.1"
                     /translation="MSERTRKVESQLREIVGEEVDSLSDPRINGLVTVTGVDVSPDLA
                     HATVFYSVLAGEDTEEAHDGLQSAAGRIQAAVGRQMRMRRTPRLRFEPDPVVETVSRI
                     EAALREVKAEHDDTDDR"
     gene            4388..5350
                     /locus_tag="VFJ72_00425"
     CDS             4388..5350
                     /locus_tag="VFJ72_00425"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET7477955.1"
                     /translation="MTTQTTGNTIREIADFLKTLDRVAITTHVGADGDAIGASAALLH
                     LMRQLGAEAVFCHSEEVPNYLQWLIPDALRELPDGHDLLVVDTSRADRTGVPIPEAGA
                     RLNLDHHEDNPLYAEHNIVRPRAAASAEIVADLYAELSVPLDKQAAEAIYTGVRTDTG
                     GFRFRNISPEVHELVAELLRVGVVPAEVDDRINRTGTIEQLNIVGVTLANAVRYGEAI
                     IATVDNDDYERTGATELDSKESIDQLRTVAGVDVVAHLREVPQGTKGSLRSETVDVGE
                     IARRFGGGGHRLAAGYTRPGMRPDEAREELLDALKGVVDLGETR"
     gene            5347..6225
                     /gene="truB"
                     /locus_tag="VFJ72_00430"
     CDS             5347..6225
                     /gene="truB"
                     /locus_tag="VFJ72_00430"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7477956.1"
                     /translation="MAEPPSNGALALDKPSGITSAGAVARVKRLLPKKSRIGHTGTLD
                     PLASGLLVLMIGRATRLSRYVTDLDKSYTATARFGATSDTLDADGEITALNLPMPDER
                     SIREALPRFTGDLLQTPPMASAIKVGGERLYNLHRRGITVERESRPVKVTRFDLASLD
                     PDRESATFEIRCSSGTYVRSLVSDLAASLETGAYLTALRRTSVGNIHVESAISPDELT
                     PEDIFNRIIPSGAVLDRLPVLEVGGESVAAICNGRPLCPCGIEGSFRVESGGELLAVY
                     RGDGFEGRAEVVLCAP"
     gene            6213..7109
                     /locus_tag="VFJ72_00435"
     CDS             6213..7109
                     /locus_tag="VFJ72_00435"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET7477957.1"
                     /translation="MRSVVVALGNFDGVHLGHQAVLRRAVEEARLRGERVVAATFHPH
                     PRAVLRPGEEPKLLSTLETRRELLVCYGADEVYVIRFDKTLSQKSPEEFVRDVLVGEV
                     GAGAVVVGENFRFGHRAAGDFDDLRRLMRDHGGDAISVPLRAANGQEGINSTRIRAMI
                     SAGDVVGAGRLLGRPYAIRGEVVAGDHRGRSIGFPTANLLPDASVTVPARGVYAGYAR
                     VGESQYAMCANIGVAPTFDRRESKIEAHLLDFDADLYGRVMEVSFVARIREEQRFSGV
                     EELKEQIARDVEEARRLADDAP"
     gene            7181..7447
                     /gene="rpsO"
                     /locus_tag="VFJ72_00440"
     CDS             7181..7447
                     /gene="rpsO"
                     /locus_tag="VFJ72_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7477958.1"
                     /translation="MAVVENKTEIIQEHRTHDDDTGSPEVQVAVLTKRISHLTEHMRE
                     HKHDFHSRRGLLKLVGKRRRLLKYLQKKDVERYRALIAKLGLRR"
     gene            7491..9548
                     /locus_tag="VFJ72_00445"
     CDS             7491..9548
                     /locus_tag="VFJ72_00445"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7477959.1"
                     /translation="MRLEIPVGERSVVLETGKLAKQAGGAVTVRLGDTMLLSTATRSR
                     HPRPGATFLPLSVDIEERMSSAGKIPGGFIKREGRPSERAILTARLTDRPLRPLFPKN
                     YHHDIQVVGTVLVADQETPYDTVSMVGASAALALSDLPFDGPIGAVRVGRSLDGEFIL
                     NPTYQQIADSDLDLVVAGTKEAITMVEAGANEVPEDVMVEALQIAQETVRAQAEAIDQ
                     WAREHGKEKQHVEPVEENPFYGELRDGYLGRIKDSIVHTDRRARNEALDEIRDELVEG
                     RDEAEVPQILDVFSTLEKEAFRELYLKDRKRTDMRALDEIRPTSAEVGVLPRVHGTGL
                     FTRGETQVLSSLALADLGLAQRLDTIEPQTGKRYMHHYNFPPYSTGETGRLGSPRRRE
                     IGHGALAERALLPVVPSEETFPYALRIISEVLESNGSSSMASVCGSTLALMDGGVPIK
                     APVAGVAMGLVKEGEDYVILTDIQGLEDHMGDMDFKVAGTSDGITALQMDMKITGVSA
                     DLLKEALGQARTGRLQILETMREAIAEPRPEVSDFAPRVEVLKIPKDKIGMLIGPGGK
                     TINALQEQFGVNISVEDDGTVFVAGEGVVSKEAASAINGMMKDVEAGEIYTGKVVKTT
                     NFGAFVELTPGRDGLVHISRLAPGKQRVEKVEDVVTEGDRIKVRVLEIDKQNRISLEK
                     LED"
     gene            9552..10814
                     /locus_tag="VFJ72_00450"
     CDS             9552..10814
                     /locus_tag="VFJ72_00450"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET7477960.1"
                     /translation="MSDPNIRQKQLPGGLRVFTEPLDEATSVSLGVWIRAGSRDEKDE
                     VAGISHLMEHMLFKGTPSMNALQVAEAFESIGAQENAATGEEYTVLYARFLPEHLEKA
                     LDIMSDMVLRPTLADLEREREVIVEEIKMYEDRPDQLADEYLSSLIFHDDTLGRPIIG
                     SAETVRGVDHDTLQRFHGSTYNASNVFVVGAGKLDQDEFERMVEEKLSGLPEGEPFVR
                     EADPKQPESRFFYKFKETEQYHVSLGSLGIPAKSEDRYAMSALNNVLGGGMSSRLFQE
                     VREKRGLAYAVYSYHQGYSDTGAIKTYVGSTTGNVEEAVKVITEQLEKIQHELVTDEE
                     LERTKQQLKSSTLLALESTAVRMTRIGRGIITGSELLTPEEISDLIEAVTPQDIERLA
                     KKHLKLDNMYLSAVGPKELDLGQYLSKN"
     gene            10920..12563
                     /locus_tag="VFJ72_00455"
     CDS             10920..12563
                     /locus_tag="VFJ72_00455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET7477961.1"
                     /translation="MQVIPLGGLGEIGKNMTAVRQESGIVLVDAGMAFPDEEMPGIDL
                     VLPDFSYLREHADELKGIVLTHGHEDHVGAVPYLLREFNVPVYATKLTLGLLRSKLQE
                     FGIKKGDLREIKAGDTQNLGGFGMEFINVNHSIPDAVAIAIRTRAGLIVFSGDYKIDL
                     TPTEGDPMDLARLAALGTEGVLAYFGDSTNSERPGYVPSERVVSDTLNRVFEDADGRI
                     IVASFASHIHRVQQVVEAAKRHGRLVGVTGRSMIRNVDIARELGYLDLPQAMYVNQRD
                     VNKIPDSDIVVLTTGSQGEPLAALSRIANGTHRTIEVGEDDTVVIAAHPIPGNERGVS
                     RTINNLMKRGANVCYSPLMPVHVSGHGAKEEQKIVLSLLKPRYLVPVHGEFRHLRHHA
                     NTGMSMGIPEENVFILENGAGVEFNNGNAKRMKPVEAGMFLVDGGGLADDSESVIRER
                     QQLAEDGMFIVVARINGQTGQLLGPPDVISRGFANPQGSNGLVEESINVVGKTLERTA
                     KQRLTDWSELKSAIRKDLSGFLSQRTRKRPIILPLIVEV"
     gene            12663..14975
                     /locus_tag="VFJ72_00460"
     CDS             12663..14975
                     /locus_tag="VFJ72_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7477962.1"
                     /translation="MTKAPAKKHRGSKNARKGMVSRFWERVRGWLAPEVIGGVLLAVG
                     LFFTAAFVSGRGAWLGDTGLMAATHLMGVAGYALAPLGALAGGLLLLQRLPAVRTFGG
                     GLILLGFATSMAARLPEGRLFEAGEYDAAGGIVGSAIYGAVYWTGGVVGAAVALVFLY
                     AVGLSLLTGISFESALGASRTFLTSSLDSLREWRKRRAEAAEKAAAEQRKSIEIPSRS
                     SRAKKAPKPAQGVVDLYPELDKEESAKRAVPESQESFEVVLPEERQEPAGEPVIHQES
                     EGSASGEYTPPPMSLLKLGGGGPHHDAEGTSQRLTQALADLGVAAQVVRAVVGPRVTR
                     YELRLGSGVKVGKVSNLQQDIAYALAATEVRILAPIPGKSAVGVEVPNTRPATVTLGD
                     VFQDYPEGNDWALPVGLGKDISGRAVFLDLADMPHLLVAGTTGSGKSVMLNGLLTSLL
                     LATDPREVKMVLIDPKRVELSQFARIPHLITPVVTDVKKAANALGWAVAEMERRYEVL
                     EQKGVRSLEGYNARAEYPMPYVLVVIDELADLMMQAGAKVEDAIVRLAQKARAVGIHL
                     VVATQRPSVDVITGMIKANIPSRIAFAVSSQVDSRVILDTSGAEALLGMGDMLYKPVS
                     AARASRVQGAFISEAEVERVVSASVAAARGKAETEFVEEVTAPKNEKQETGEPEDELL
                     PEAASFVVATQQASVSAVQRRFRVGYSRAGRIIDALERKGIVGPYEGSKSRSVVASEL
                     ELSSIFSGESSDGGGSGGPSGTGEKQSESW"
     gene            15022..15897
                     /locus_tag="VFJ72_00465"
     CDS             15022..15897
                     /locus_tag="VFJ72_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HET7477963.1"
                     /translation="MYERDGGLEIGPTLEKARLQRELTLDEVEQATKIRKRYIQAIEK
                     DDYGVLPDAVYVQGFVKTYANYLGLNGEELAGELRSRRQSRRERHVNHARLTKGGFDK
                     PLINPGGVAGAEKRRISGGAVLGLLASILLIVLVIGALYYVGRGSRVQVPDDGASAPQ
                     AEQADKNSDKPESGKPDAGVSSTGSAGTAGGTKKAGGNEAGNAGAETPAPVEVAIQVE
                     GSPAWISVKSDGTVVYEQVADPGFSDTFEGEQTVGISTGNAGAVKLKVNGKDEGAMGG
                     AGEVVSREFTPESES"
     gene            16010..17074
                     /gene="recA"
                     /locus_tag="VFJ72_00470"
     CDS             16010..17074
                     /gene="recA"
                     /locus_tag="VFJ72_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET7477964.1"
                     /translation="MDKSKAIGAAVTQIERQFGKGSIMRMGDRGTQKIPVIPTGAMSL
                     DLALGVGGLPRGRIIEIFGPESSGKTTLALHVISEAQKAGGLAAFIDAEHALDPAYAE
                     RIGVDLENLYFSQPDNGEQALEIADTLVRSGALDLVVIDSVAALVPRAEIEGEMGDSH
                     VGLQARLMSQALRKLSGSLNRSGTTAIFINQLREKIGVMFGSPETTPGGRALKFYSSV
                     RLDIRRIGALKNGNDTVGNQTKVKVVKNKVAPPFKVVEFDIMYGEGISKEGSLLDIGI
                     EQGVVQKSGAWFGYGEERLGQGRENAKQFLKENDAVRERIVHDIYDKLGLNAIEGGEE
                     SNGEGVSPERPAKEPAGQVL"
     gene            17091..17720
                     /locus_tag="VFJ72_00475"
     CDS             17091..17720
                     /locus_tag="VFJ72_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HET7477965.1"
                     /translation="MPQITGVRERRGRARVFVDGEFWAEIDANVALERGLYEGAALSC
                     DELDEARVAGEKALAMSRALNFLAYRSRSEKEVRDRLFRYGYGAETVDGVLERLAELG
                     YLDDEGFARTAAREKSRKYGPRRVSADLMRGGVSGEIARAAVEEAFAEHSEREMAREL
                     AARRYNKSERSDAQARRVYGFLVRRGFSADVCAGIAREYRGPDASEADV"
     gene            17777..19321
                     /gene="rny"
                     /locus_tag="VFJ72_00480"
     CDS             17777..19321
                     /gene="rny"
                     /locus_tag="VFJ72_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET7477966.1"
                     /translation="MNVLLLVLGLAVGLLGGAALGYYVCKSRMEARQAEARSDARSLI
                     EDANRQVEASRREAELAAKEAALKVKDEAEAELRSRRAEIARVEERLQNRDATLDRRE
                     SELDRRRQKVSETEEALQRREEDLKSREAEQLRALEEISGLTRAEAERRLYSGLEQDL
                     EDRLGRMVRDRTLEAEERADNEARRIISTTMERLASDLTSESTVKAIALTSDDMKGRI
                     IGREGRNIRAFEAATGVDVIIDDTPETVVISCFDPVRREIARVSMERLVQDGRIHPGR
                     IEQIVAKAKKDVEKEMKAAGRQALYDAKISGGMNNDLIRLLGALKYRTSYGQNVLAHS
                     VEVANLCGMMAAELGANVKISRRGALLHDVGKAIDHEVEGTHALIGGRYAKKSGESED
                     VVRAISAHHHEIEMETVEDVLVATADAVSAARPGARRETTETYIERLRSLEDIALGHR
                     GVDKAYAIQAGREVRVMVQPSEVDDRIAAKLAFDISKQIEEDLEYPGQIKVTVIRESR
                     VSEVAR"
     gene            complement(19408..19602)
                     /locus_tag="VFJ72_00485"
     CDS             complement(19408..19602)
                     /locus_tag="VFJ72_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477967.1"
                     /translation="MAAGHSRLEDHLRVAVIQALPVINNGQSPVAATAQDRGDVNAAS
                     SGVAGVPEQLHQRVFHGGDT"
     gene            complement(19662..19808)
                     /locus_tag="VFJ72_00490"
     CDS             complement(19662..19808)
                     /locus_tag="VFJ72_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477968.1"
                     /translation="MARRLDLDARLGDWRTRSGKVIVSKVAENDVGDEAETQAALNAR
                     VRIW"
     gene            20116..20256
                     /locus_tag="VFJ72_00495"
     CDS             20116..20256
                     /locus_tag="VFJ72_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477969.1"
                     /translation="MARIAAILDEAARASYLEVRDLRQHSRPGEIRLSARVEEPEDLY
                     EQ"
     gene            20246..21616
                     /locus_tag="VFJ72_00500"
     CDS             20246..21616
                     /locus_tag="VFJ72_00500"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HET7477970.1"
                     /translation="MSSRTEAEQAVGQAAGQAVRVALSTKPAKGVRPGSGEARTACIR
                     TFGCQMNVHDSDRIRRMIRDAGYAEVPTYEEADLVILNTCYVRENAVDRVRGHLGELT
                     RLRREGRVKRIALTGCIGAAEESSELKRQYGIDLVLGTHNTYELAEFVGLPTMEETYT
                     PDLPGTEGDNSAFVTIMTGCNYQCSYCIVPTVRGRMVCRPMDNILEEVRRLVESGTSY
                     ITLLGQTVDAWREDGRRFCDLLERVSEIAPRVWFTTSHPSNMEDRTLRLIGEKDTIVK
                     RLHLPVQSGSDRMLRVMHRGYKSDRYRRKIELFREVAPEGTLSTDLIIGHPGETEEDH
                     QETLKLVEDCSFDSAYVFKFSPRRGTEAASIQESVPDDVVQRRFLQVLRKIEQNAFNR
                     NRTRIGRVEDIYIRYERNRDGVAIGETWNGHAVHVETDAPTGQYVRAEIKAAGPHVLY
                     AGETLI"
     gene            21724..22470
                     /locus_tag="VFJ72_00505"
     CDS             21724..22470
                     /locus_tag="VFJ72_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477971.1"
                     /translation="MVDSMQVYREIPTITNQRRQRPAEMVGVASVLDEWTVARHRDMS
                     LGILAGIDIPFVLDAGTGMYLNALLLEIELSPKVEPHIRAVAESLSQGDVNPRRASRE
                     RELRLAGAPERSSIWDANLRYETTLIYIRPDRESLDKNIQRRANHIIQNAIAESETIA
                     ALISRGATLSPQVSGAIGVKEMLDLVAGSLTGAEAESRIAARTRRLARRQIRWFDKLT
                     RTISDRAAVEVVSHSSDAETMHTLHDTIGS"
     gene            22467..23723
                     /gene="hflX"
                     /locus_tag="VFJ72_00510"
     CDS             22467..23723
                     /gene="hflX"
                     /locus_tag="VFJ72_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET7477972.1"
                     /translation="MSDTENTGIDRAVLVGAGEGRYLDELGRLAGTLGVKVVAEVEQN
                     RRDGSGYLGKGKREELRDVVAEAGAAMVIADDELTASQARVLERSSGASVVDRTELII
                     RIFEAHARDSASRLEVELADLQYRLPRVKGRNEALSRLGGGGVTTRGPGEQQLEYDRR
                     VIRERMSSIERKLEEERSARAVQGSRLRKSGPPTVALVGYTNAGKTTILNRISGAGRS
                     TMDRLFETLETTSRLVEGRADGKVARPDFVLTDTVGFIRKLPKQLVRSFESTMEVARD
                     ADVRVLCADASSAELEDEISTVREILDDENAGESGVNNILCLNKWDLISKERGSDLRA
                     EYPDAVTISARNGETHPLLERIYDSIAGGRERMNLLIPHSEYANAARLYGVAEIHSSN
                     DTTTGVCLDVTLPKHVARRYGRYRVA"
     gene            23735..24502
                     /locus_tag="VFJ72_00515"
     CDS             23735..24502
                     /locus_tag="VFJ72_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7477973.1"
                     /translation="MTAAIKIYPPPSETFRVEDLYTDIEFPDHNITDPSLPYVVINMV
                     SSLDGKAALNGVANGIGGPADRRVMRNLRSRADAVLRGAGTLRAEKISAGVPEDLARR
                     RMSEGRPAQPLELILSRSDTASLSNLLNASSGTVALVTQDRGPVKNLEDMERIVVPAR
                     PDGLVDLVKAMIILKRDHGLSVILLEGGPSLNHALISSGLVSELFLTLSHRLLGGSQD
                     QILTLLNGQPLPPELSTPLRLISAHLTDDEWFLRYKM"
     gene            complement(25245..26204)
                     /locus_tag="VFJ72_00520"
     CDS             complement(25245..26204)
                     /locus_tag="VFJ72_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7477974.1"
                     /translation="MTTRKTNLPAPLESEVATLSPTSTEERLLSVYLRTLTSPQTIKT
                     YNTEIRMFLAYTREIGKDLEQITAEDISLYREHLLKTYASATTAKKLAALRRFLVFTY
                     MGGATSVNPEALRFFAKSPRVRQDPSYNVLTEDELARMLSAARATSYRDYVMLAVMAS
                     CGLREAEVVGIKVGDFRDVGGGQVLLRILGKGDKIRNVPISPELWQLVQRFVLLSGRS
                     FTSHADARKPLFISREGKDRSLTTRSIRYIVKKYVNSAGITKAISPHSIRHTVGTNMA
                     VNEAPLLVIQQYLGHSDPKTTMRYIRRAEELASKAYQYNTLPI"
     gene            complement(26185..26733)
                     /locus_tag="VFJ72_00525"
     CDS             complement(26185..26733)
                     /locus_tag="VFJ72_00525"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET7477975.1"
                     /translation="MASGIDVREIERHIRTVPDFPKPGISFKDITPLIEDGPALRAAV
                     DSLASATEDIDYDRIVSAEARGFVFGTALAYRAGKGLILARKPNKLPRETIAASYELE
                     YGFETLEAHLDSIQPGTRVLVMDDLLATGGTAQAMCELVESAGGVVAGAAFLIELSYL
                     NGRERLAPHDVVSLITYDDPED"
     gene            complement(26733..27401)
                     /locus_tag="VFJ72_00530"
     CDS             complement(26733..27401)
                     /locus_tag="VFJ72_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7477976.1"
                     /translation="MENGGNKDSYTIGEVVTRLRQDFPTLSVSKIRYLERRRLINLSR
                     TRGGYRLFSNQDVELLRYILTLQDKEYLPLKVIKKRIEGGAPVPSTGEGGGDLSRVLE
                     DRVYSREELADALEADSRFVDDLISVGVIGRTGELTQRDVEIARMALEMSKYSIQPRH
                     LRGIMAAVDREAAMFKQILNPELRSSDEVRVAEAMNKARHLAAVDSRLKELMMASIIE
                     SFVP"
     gene            complement(27454..27909)
                     /locus_tag="VFJ72_00535"
     CDS             complement(27454..27909)
                     /locus_tag="VFJ72_00535"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7477977.1"
                     /translation="MNVCPECGSPVNERMKFCAECGAKLAHGQSTVSYSPEFIEDEDT
                     SGASAGGKAGAALIELDQVEGTAGRRMHDISEEVVTVGRSPSCNIFLDDVTVSRNHAE
                     ILQSTRGFRISDSGSLNGTYVNRVRVDSVDLRNGDEIQVGKYRFKFVNT"
     gene            complement(27906..28169)
                     /locus_tag="VFJ72_00540"
     CDS             complement(27906..28169)
                     /locus_tag="VFJ72_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477978.1"
                     /translation="MGSEGFEWEDVDDVTSLSEEELRGRLEALTGEEREISYRRRILQ
                     GRIDLIRAELVRRGDAALSPQELARVLMDRTGGAQEEQGGGPA"
     gene            complement(28270..28818)
                     /locus_tag="VFJ72_00545"
     CDS             complement(28270..28818)
                     /locus_tag="VFJ72_00545"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7477979.1"
                     /translation="MPRVEDRNPPSDRVMTLPNALTLFRLLMVPVVVGLLLAEADGIA
                     VAVFVAAALTDFLDGRIARRQGPTRLGQLLDPVADRLMLSSSAIVLAIRGLLPVWAVA
                     ILVGRDVLALLGSFLVRGKMRVNVVGKAATAVLMVSVGIVIFSPGALGETIFYAGCVL
                     SLAAAALYAVGFYLLADRGEIP"
     gene            complement(28851..30155)
                     /gene="der"
                     /locus_tag="VFJ72_00550"
     CDS             complement(28851..30155)
                     /gene="der"
                     /locus_tag="VFJ72_00550"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7477980.1"
                     /translation="MSRLPVVAIVGAPNVGKSSLVNRILGRRQAVVSGEPGTTRDRSY
                     NRAEWAGREFTLVDTGGMEPTAKVGLEALVTLQARIAAEEADVILHLTDSRLGPTEAD
                     AAIARELRRAPGSVVLAVNKLDDPANDADRHLFHALGEGEPYAVSAAHGLGTGDLLDA
                     IVERLPETEADEEDKIPRLAIIGRPNVGKSTLLNRLLGAERAVVSDVAGTTTDAVAGE
                     VEVKSPEGIEKYLLLDTAGVNRSARRADGVPYYSSLRTEEAIRRSDVSLLLVDAVEGL
                     VGGDLQLARRIEEAGRTCGILINKSDLVPRERLQEIEDSLYARMTDLKPHAISISALT
                     GAGVKEVLPFAARLRQAYNLRVPTHEIADLVNRLVDRSAPPRGVKIRYATQTGSAPPR
                     FMLFANRPKDIPDSYLRYLENALRERYDLYGVSVRLRVRSSR"
     gene            complement(30152..30730)
                     /locus_tag="VFJ72_00555"
     CDS             complement(30152..30730)
                     /locus_tag="VFJ72_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7477981.1"
                     /translation="MIGRVLFGFTVHGAENVPKTGPLILASNHSQYADPVLVNMAVPR
                     RVQWMGKKELFVFPFGKFFRFIGTFPVDRQSGGRAALRAALDYLADGWTLGIFPEGTH
                     RNHGISRDAKSGVVMLSTRGNAPVLPIYMSRIPNLAQRMRGAKLHAYIGEPMTTDNTT
                     RGGRAYREAADEILRTIYALPEKHAKEVGDGS"
     gene            complement(30813..31478)
                     /gene="cmk"
                     /locus_tag="VFJ72_00560"
     CDS             complement(30813..31478)
                     /gene="cmk"
                     /locus_tag="VFJ72_00560"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7477982.1"
                     /translation="MGGLLVTIDGPAGSGKSSVARAVAERLGVVNLNTGAAYRAVALL
                     ALRDGVAFDDGAGLARLARAVDLDPDGVRIDGERVQDPDLRTPEVSAAASKISAHPEL
                     REVLLTVQRRAAERARQQGGAVVEGRDIGTVVLPDADLKVFLSAAPEERARRRAVQSG
                     REGELERIRKAIETRDRQDSEREVSPLRPAPDAVVLDTTELTLDGVISKVLDLARQTR
                     SRV"
     gene            complement(31513..32847)
                     /gene="aroA"
                     /locus_tag="VFJ72_00565"
     CDS             complement(31513..32847)
                     /gene="aroA"
                     /locus_tag="VFJ72_00565"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET7477983.1"
                     /translation="MRPVSGVPGEDFSVEDVRGSFPQEIEIKPLNGPVDAEIPVPGSK
                     SVTNRALIVAALASGASTIRNPLFSDDSYWLLAALTQLGFAVNVDRTRAVAHITGQSG
                     IIPRKGMDVYVGNAGTVARFLPPALALGEGPYRVDGVARMRERPVDDLVDAMRQLGAD
                     VGYGGEEGRFPLVVRGGGLRGGEARVEGSKSSQFLSGLLMAAPYARARVELRVGGDLV
                     SRPYVGITTGVMRDFGVQVDVSRDGRYVIEPGYYRAGEYVVEPDASGASYFFAAAAAA
                     GGRVRVPGLGSGSTQGDLRFVEVLREMGCEVDMTRDYVEVRGTGRLRGVEVDMNEFSD
                     TMITLAAIAPLAEGPTRITNIEHTRHQETDRISAVATELSRMGVKVREERDGLEISPG
                     PVRPAQVRTYGDHRIAMAFAVTGLVAPGITIQDPGCVTKTFPEYFERLDSLR"
     gene            complement(32844..33545)
                     /locus_tag="VFJ72_00570"
     CDS             complement(32844..33545)
                     /locus_tag="VFJ72_00570"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7477984.1"
                     /translation="MRLQTYLARAGASPSRRKGEALISAGRVTVNGSTATIGTTVSSS
                     DSILLDGRAVRLPQTTAYLALHKPAGYLTTLKDEPGRDRRTIRDLMPSVPGLVPVGRL
                     DAATTGLILLTNDGKFAHRISHPSSEVEKEYLLTVNSPVSDATLAALAAGPKLDDGKM
                     LPPVITSVRRGPRNTTITLTIHEGRNRIIRRACAVVGLGLISLHRTRIGPVRLGDLPP
                     GRHRSLTSSELKALQ"
     gene            complement(33546..34169)
                     /locus_tag="VFJ72_00575"
     CDS             complement(33546..34169)
                     /locus_tag="VFJ72_00575"
                     /inference="COORDINATES: protein motif:HMM:NF016005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET7477985.1"
                     /translation="MSEPPTPAPATPAARIEAVLLVSPRPVSEAALKSAAGLSDSELR
                     DAMGLLGERYSPRESGIVLRRVAGGYQLATNPVCASAVERFREESRPAPLSNAAHEVL
                     SCALYLGPLTRAEISAARGVNSDAVVRGLIERGLLAETGSDRESPGAPALLDVTDEFL
                     AATGATSREDFAPLDSLVGPEELARVRERVGAAETGSEQEPATPEES"
     gene            complement(34166..34939)
                     /locus_tag="VFJ72_00580"
     CDS             complement(34166..34939)
                     /locus_tag="VFJ72_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET7477986.1"
                     /translation="MKPSAVKPSAVNPSAVQSAGAPGRSLADFTVELDVYSGPYEWLL
                     ALIMKDELEIFEIPLRELVETYLTSHGSGERPHSLERDTDFVSSAASLVLLKSRTLSP
                     IFDAEPEAEEEALTPDQLAEKLTGYLRIRRGAESLRERFAANAGFYPTAQTVQPRPGR
                     LKIRTERLEAAARRALSRIVEPPVRHLGPITVTVQELAALIRTSLARGPVSFEVLVRD
                     MDRLHSAVAFAAALSLAHEGRLRLLQTEPLGPLTLEAPA"
     gene            complement(34936..36069)
                     /gene="ald"
                     /locus_tag="VFJ72_00585"
     CDS             complement(34936..36069)
                     /gene="ald"
                     /locus_tag="VFJ72_00585"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7477987.1"
                     /translation="MRIGVVKELTPGESRVALAPHAVNELARSGHRVLVETGAGEASS
                     ISDAEYEQAGARLVSDPAVVYSESELVAKVYGPIREEHQNLREGLILISFLSLPVNPG
                     LTQALVESRCVGFAMESIRDGRGSLPILTPMSEIAGRLVPQIGAHYLQRPAGGRGILL
                     GGSVGVKPGRVVILGCGIVGSGAARMASGLGADITVIDRDLHRLRLLENARLRNVTTL
                     SASAMSIQDAVLRADLLIGAVFVAGSRTPALVDRATVAEMKPGSVIVDVDVDYGGCVE
                     TSRPTTLDDPTFVESGVIHYCVKNVPAAVPVTATRALSNALLPSIRQVAGIGLIDALN
                     AETGLRRGVVVAEGRIVDRTLARTIGSEHHPLSSVLPLHSEVR"
     gene            complement(36066..37043)
                     /locus_tag="VFJ72_00590"
     CDS             complement(36066..37043)
                     /locus_tag="VFJ72_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HET7477988.1"
                     /translation="MEVQEDRFSYSIEILDGPLSGEVHRGVFYPEMGAAPGPGNEVIV
                     NTVGLEMDLGTGGFAVILPSAGGEAPRNEDHFVKMPYTPLQYPVSPARQALTLEGVRV
                     VVLPLHSHLAPACCAAADLRPGVRTAFVWQEGGALPVEFSRTARVLRDKGLLDIVISC
                     GNCFGGDLESPNVYSGLLAAAAVADVVIVGIGPGLIGTGAPYGHGGMSSAIALNAALA
                     LGAEPVLAPRVSAADPRERHRGLSHHTTTVLRATLAPCRVALPSGTGISVAGLTDQHD
                     CVEVAYTAAGLEERFGLTFESMGRSYEDDAGFFDAAAAAVSLALGKGVE"
     gene            complement(37076..37513)
                     /locus_tag="VFJ72_00595"
     CDS             complement(37076..37513)
                     /locus_tag="VFJ72_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477989.1"
                     /translation="MEWRSTAEMPRPTGLLGAAARNAAWTEDPEGAARTLRAEIIALT
                     GPALGAALEDALRRIAAHAALTDAVLQVRAVHGLPPRIVPGETLAALARDLYGAGFRV
                     CFAPMMAPAPGDGVPLGMRGAERELREFVESHPEWELPERGIA"
BASE COUNT         7216 a        11474 c        12613 g         6434 t
ORIGIN      
        1 ggtgagagag acggcgaggc tggagagatt atcggcggtt gtagaggggc ttttgccgga
       61 ggatgcggag cttgtagagg ctcgtttctc cgggggaccg ctgctgacgc tgctggtcga
      121 ccggcgcgat ggcctggtgg atcacgagtt caccacgcgg gtcgtctccg ctatctcgcc
      181 ggcgctggaa gaggagggct acgacggcat ggtcgaggtc tcctcgccgg gtatcgagcg
      241 gcctctgacg aagccggagc attaccggcg cttcgtcggg cacgaggcga aggtccgggt
      301 cggagagccg atagacggcc ggcgcaattt cacgggcgtc atagaccggg cggacgatga
      361 aggttttgtc ctacaactcc ccgaggagga agtggaggtg gagttgccgt accgctccgt
      421 tacccgcgcc aacctcaagg aggacatata acgatatgaa cacggcacta ttatccgcgc
      481 taagagagat agagacagac aagaacatcc ccttcgacac catcaccggt gtcctggagg
      541 agtcgctcct ggccgcctac gagggacgtg agtcggctga ggagggggcc gaggtggtcc
      601 tcgaccgcga gaccggcgac ctgcgggtga tgaaggacgg ggaggacata acacccgcgg
      661 acttcacgcg catcgccgct caggtaatgc gccagacgtt ctaccagcgc cttaatgaga
      721 tccacaacga gcaactcctg gaagagtacg ggagccggat gggcgacgtc gtgaccggca
      781 tcgtgcagca gtctcatcgc cggatgacgc tggtggacct cggacgggtc gaggcgctgt
      841 tgccggcgag tgagcaggtc ccgaacgagc gctacgagaa tgggcagagg atcaaggtct
      901 acctcctgga gatccgggag ccgggtcgcg gcccgagcat gaccgtgagc cgccgccacg
      961 aggggctgct gcggggtctc ttcgagctcg aagtcccgga gatctacgac ggttacgtcg
     1021 agataaaggc cgtggcccgc gaggccgggc tgcgctcgaa ggtcgccgtg tactcgaagg
     1081 agagtggtat agacccggtc ggggcgtgcg tggggccgcg cggaagccga gtgcgggcgg
     1141 tcgtctcgga gctgaggaac gagaagatag acatcatcca gtgggacccg gacccggcgc
     1201 gctttatagc gaaggcgctg agtcctgcgc gagtgcgcga ggtctacctg gatgaagacg
     1261 agcggcaggc cgaggtgatc gtcccggacg accagctctc gctcgcaatc ggacgcgagg
     1321 gccagaacgc ccggctcgcg gtgaagctga ccgactggaa gatagacata aagcccgcca
     1381 gccaggcgac agagttcgag gacgaggaag aggactggga gccggatgaa gactccgcga
     1441 tgcaccgctg ccgggccgtc ctctcgaacg gtcggcgctg cgcgaacatg gccctgccgg
     1501 gttcgctctt ctgcggtatc ccgtcgcatc aggcgcaggc cgcggagttc gagggcatgg
     1561 tagagggcac gcaggaggta tcggatgagt aggcgcgttt acgagatagc aagagagctg
     1621 ggactgaaca ataaagaggt tttgaaccgt ttcaatgcgg ccgggatcga ggtaaagagc
     1681 cactcttcgt ccgtggacga ttccgcttac gagcaggtct tcggaaatgg cgctggagtg
     1741 gccaacggtc gcacggaggc gcaggaggcc gaggcgcagg aggcgcgaac cgaagcagag
     1801 caatcgggag atggcgcagc ggtctccgac gcgccggccg aggagccggt cgcggtcccg
     1861 gatgacaggc caaagaaaca gcgcggcggc ggcaagaagc ggcgccgggt cgtaatcgac
     1921 gcgagcgcga cgaatcgtgg tccgcgtccg accgcggcgc cggccgcggc gaaggagact
     1981 tccaggccag cggcggagga gaagcctgag atcagcgcgg tgcgcgtcga gcccggcgcg
     2041 acggtcaaag acctggccga cgctctggac atcgccccga cacaggtcat caaggtcctg
     2101 atgggcctcg gagagatgaa gaccgtcacc cagaccctct cgaccgagga gatcgagctt
     2161 acagccgagg tgcttgggcg cgaggtcgag gtcgccgccg tcgaggagcc cgcgccggaa
     2221 gaggtgctgc cggaggatga tccgggagac ctcgtggaga agccgccggt cgtgaccgtg
     2281 atgggccacg tggaccacgg caagacatct ctgctcgacc gcatccggaa ggcgaacgtg
     2341 cagtcgggcg aggcaggcgg catcacgcag cacatcggcg cctaccaggt cgaggcgggc
     2401 gaaggcgacg agcggaagaa gatcacgttc atagacactc ccggtcacga ggcgtttacc
     2461 gagatgcgcg cccggggcgc gaaggtgacg gacgtggtcg tgctggtcgt cgcggccgat
     2521 gacggtatca tgccgcagac gcaggaggcc atagagcact ccagggcggc tggtgtgccg
     2581 ctggtcgtgg cgctaaacaa gatcgacgtc ccaaacgcaa acccggaccg ggtactcggc
     2641 cagctctccg agcagggcct gatgccggag gtctacggcg gcgagaccgt tacggtcccc
     2701 gtctccgcca agaccggcga ggggatagac gagcttctgg agaacatcct tgtagtctcc
     2761 gaactggagg acctcaaggc caacccgaac gccccggcga gcgggtacgt gatcgagggc
     2821 gagcgcgacc ccggacgtgg gccggttgcg actctccttt tgcgccgcgg gacgctccac
     2881 aagggcgacg tagtcctggc cgggacggcc tacgggcgcg tccgggcgat gctcgattac
     2941 accggaaagc ggatcaagga ggccggtccc ggaagcccgg tcgagatcct gggtctctcc
     3001 ggcgtcccgg aggcgggcac gcgcttcgag gtggtggaac acgagcgggt cgcgcgcgac
     3061 aaggcgcagc aggccgaggc cgctctcagg cggcaggagc ttgctcaggg aggctcgcgg
     3121 cgcacgctgg aggaactgct cggcgaaggt ggcgtccagg acctcaacct ggtcgtaaag
     3181 gcggacgttg ccggctcggt ggaggcgctc aaggaggcgc tcgccaagct ctcgaccgac
     3241 gaggtgcgag taaacatcgt gcgcagcggc gtcggggcgc tcacggactc ggacgtcatg
     3301 ctcgcgagcg cttcggacgg catcctgctc gggttcaacg tgcggccgac taacacggcc
     3361 aagcaggtcg ccgagcggga gggcgtggag atccgtacct acgacgtcat ctacaaggcg
     3421 ctcgaagaga tcgaagccgc catgcgcggc atgctcgcgc cggaggagag ggagaacgag
     3481 accggcaccg cggaggtgcg tcagatcttc cgcgtcccga atatcggggt tgtggcgggc
     3541 tgttacgtca cgagcggcga gatcttccgc acgaaccggg tccgtgtgat ccgagacggc
     3601 gtggttatct acgagggcaa tatcgcctcg ctacgccgct tcaaggacga cgctcgttct
     3661 gtccggcagg gtttcgagtg cggtatcggc atcgagaact tcaacgacgt gaaggaaggc
     3721 gacgagttgg agttcttcga gatcgtggag atcccgaggt aatcgggagc cattgttctg
     3781 tgccatgagg cttgagctgg agttgccgtt tgcctccagc ctcaaggata agcgcaagac
     3841 gatacgctcc ctgaaggacc gcctccggcg gaagaacgtc tccgtggtcg agtcggacaa
     3901 ccacgactcg tggcagcggg cgacgatgga gatcgccgtc gccgccgtgt cgcgcggcgc
     3961 ggcggaggag aaacgcgagg agatccggcg ttctctactc aactacgagg agctttcgat
     4021 cgtggagtgg cgcgaggagt tcgtgaagct atgagtgaac gcacccgcaa ggtagagtcc
     4081 cagttgcgcg agatcgtggg cgaggaagta gactcgctct cggacccgcg aattaacggt
     4141 ctggttaccg ttacgggcgt ggacgtgagc ccggatctcg cgcacgcgac ggtgttttac
     4201 agcgtcctcg cgggagaaga taccgaggag gctcacgacg ggcttcagag cgcggccgga
     4261 cgtatccagg ccgctgtcgg acgccagatg cgcatgagac gtacaccccg gctgcgcttc
     4321 gagccggacc cagtcgtaga gacagtgagc cgcatagagg cggccttgag ggaggtcaag
     4381 gcagaacatg acgacacaga cgacaggtaa cacaatccgt gagatcgcgg acttcctgaa
     4441 gaccctcgac cgggtcgcca tcacgaccca cgtgggggcc gacggggacg ccatcggcgc
     4501 ctcggccgcg ctgctgcacc tgatgcgcca gctcggcgcg gaggcggtct tctgccactc
     4561 ggaagaggtg ccgaactacc tgcagtggct catcccggac gccctgcgcg agctgccgga
     4621 cggccacgac ctgctcgtgg tggatacctc gcgggccgac cggacgggcg tccccatccc
     4681 cgaggccggg gcgcgactga acctggacca ccacgaggac aacccgctct acgccgagca
     4741 caacatcgtc aggccccgcg ccgccgcgag cgccgagatc gtcgccgacc tgtacgcgga
     4801 gttgagtgtc ccgctggaca agcaggcggc cgaggcgatc tacacgggcg tccgcaccga
     4861 caccggcggt ttccgtttcc gtaacatctc cccggaagtt cacgagctcg tcgccgagtt
     4921 gctgcgtgtc ggggtagtgc ccgccgaggt ggacgaccgt atcaaccgca ctggaaccat
     4981 cgagcagcta aacatcgtcg gcgtcacgct ggcgaacgcc gtccgttacg gcgaggcgat
     5041 catagccacc gtggacaacg acgactatga gcgtaccgga gctaccgagc tcgactcgaa
     5101 ggagtcaata gatcaactcc gcaccgtggc cggggtggac gtggtggccc acctgcgcga
     5161 ggtgccgcag ggcaccaagg gctcgctccg ctccgagacg gttgacgtag gggagatagc
     5221 ccgccgcttc ggcggagggg gacatcggtt ggccgcgggc tacacccgtc ccggtatgcg
     5281 ccccgacgag gcccgcgagg agcttctcga cgcgctcaag ggcgtcgtgg acctgggaga
     5341 gacgcgatag ccgaaccgcc ctcgaacggc gcgctcgcgc tcgacaaacc gtccggcatt
     5401 acgagcgccg gggccgtcgc gcgcgtcaaa cgcctgctcc cgaagaagtc caggatcggc
     5461 cacaccggca ccctcgaccc gctcgcctcc gggctcctgg tgctcatgat cggccgcgcc
     5521 acgcgcctct cccgctacgt gacggacctt gacaagtcct acaccgccac cgcccgcttc
     5581 ggagctactt ccgacaccct cgacgcggac ggcgagatca cagctctcaa ccttccgatg
     5641 ccggatgaga ggagcatccg cgaagccctg ccacgcttca ccggagacct tctacagacc
     5701 cccccgatgg cctccgccat caaagttggc ggcgaacgtc tatacaatct ccaccgccgg
     5761 ggcatcacgg tggagcgcga gagccggccg gtgaaggtta cgcgcttcga tctcgcctct
     5821 ctcgatcctg accgggagag cgcaaccttc gagatacgct gcagcagcgg cacctacgtg
     5881 cgatccctcg tttccgacct cgcagcctcc ctggagaccg gagcctacct gacagcgctc
     5941 cgtcgcacct ccgtgggtaa catccacgta gaatcagcca tcagccccga cgaactgact
     6001 cccgaagaca tatttaaccg cataatacct tcaggagcag tgctggaccg attaccggtc
     6061 ctggaggtcg ggggggagtc tgtggcggct atctgcaacg gccgtccgct gtgtccgtgc
     6121 gggatcgagg ggagtttccg ggtcgagagt ggaggcgagc tcctggccgt gtatcggggc
     6181 gatgggttcg aggggcgggc ggaggtggtc ttatgcgctc cgtagtcgtg gcgctcggca
     6241 acttcgatgg tgtgcatctc ggacatcagg cagtcctacg tagagcggta gaggaggcgc
     6301 gtctcagagg agagcgggtt gtggcggcga ctttccaccc gcatccgcga gcagtactca
     6361 ggccgggaga ggagccgaag ctgctctcaa cgctggagac gaggcgcgag ttgctcgtgt
     6421 gttacggggc tgatgaggtg tatgtgatcc gcttcgacaa aacactctcg caaaagagtc
     6481 ccgaagagtt cgtgcgggac gtgctggtcg gtgaggtcgg tgcgggggcc gtggtcgtag
     6541 gggagaactt ccggttcggg catcgggctg cgggggattt cgatgacctc cggcgtctga
     6601 tgcgggacca tggcggcgac gctatcagtg tgccgcttcg cgccgcgaac gggcaggagg
     6661 ggatcaactc gaccaggata cgggccatga tctcagccgg cgacgtggtc ggtgcggggc
     6721 ggctgctggg ccgtccgtat gcgatccggg gggaggtcgt ggcgggagac catcggggca
     6781 ggtccattgg cttcccgacg gcgaacctct tgcccgatgc gtccgtgacg gtccccgccc
     6841 gcggggtata cgcgggatac gcccgggtgg gagagagcca gtacgcgatg tgcgccaaca
     6901 tcggcgtcgc cccgacgttc gaccggaggg agagcaagat agaggctcat ctgctcgatt
     6961 tcgatgcaga cctctatggc cgcgtcatgg aggtgagctt cgtcgcgcgc atccgtgaag
     7021 agcagcgttt ctccggcgtt gaggagctca aagagcagat agcgcgggac gttgaggagg
     7081 cgcggaggct ggcggacgat gcaccttgat gcttgtggta gcatatggcg tggactagag
     7141 tgagataaat aaacggttag caaagaggag aaaaaagaag ttggcggtag tagagaacaa
     7201 aacggaaata attcaggagc accggaccca cgacgatgac acgggctctc ccgaggtcca
     7261 ggtcgcggtg ctgaccaagc gcatctccca cctgacggag cacatgcgcg agcacaagca
     7321 cgactttcat tcgcgccggg gcctgctgaa gctggtcggc aagcggcgtc gtcttctgaa
     7381 gtaccttcag aagaaggacg tcgagcgcta ccgcgctctc atcgccaagc tcggcctgcg
     7441 ccgttaagca gcgctccgag aacacccctt agcaagagga gaaagagaat atgcgtttag
     7501 agatcccagt cggcgagcgc tccgtcgtgc tcgaaaccgg gaagctggcg aagcaggccg
     7561 gtggagcggt taccgtgagg ctcggagaca cgatgttgct ctccaccgcg acccgttcgc
     7621 gccacccgcg ccctggagcg actttcctgc ccttgagcgt ggacatcgag gagcggatgt
     7681 cgtcggccgg taagatcccc ggcggcttta tcaagcgcga gggccgaccc tccgagcggg
     7741 ccatacttac cgcccggcta acagaccgcc ccttgagacc gctcttcccg aagaactacc
     7801 accacgatat ccaggtggtc gggaccgttc ttgtcgccga ccaggagacg ccctacgaca
     7861 cggtcagcat ggtcggcgcc tcggcggcgc tcgcgctctc cgacctgccg ttcgatggcc
     7921 ccatcggggc cgtgcgggtg ggacgctcgc tcgacggcga gttcatcctc aacccgacgt
     7981 accagcagat agcggacagc gacctggacc tggtcgtggc ggggacgaag gaagcgatca
     8041 cgatggtcga ggccggcgcg aacgaggtgc cagaggacgt gatggtcgag gcgctccaga
     8101 tcgcgcagga gaccgtccgc gctcaggcgg aggcaatcga ccagtgggcc cgcgagcacg
     8161 gcaaggagaa gcagcacgtc gagcccgtcg aggagaaccc cttctacggc gagttgcgcg
     8221 acggatacct gggacggatc aaggacagca tcgtccatac cgaccgccgc gcccgcaacg
     8281 aggcgctcga tgagatccga gacgagctgg tcgagggccg cgacgaggcg gaagtgcccc
     8341 agatcctgga cgtcttctct acgctcgaga aagaggcgtt ccgggaactg tacctcaagg
     8401 accgcaagcg gaccgacatg cgggcgctgg atgagatccg tccgacctcc gccgaggtcg
     8461 gcgtccttcc tcgcgtccac ggcacgggac tcttcacccg tggcgagacg caggtcctga
     8521 gctccctcgc cctcgccgat ctcggcctcg cgcagcggct agacaccatc gagccgcaga
     8581 ccggcaagcg ctacatgcac cactacaact tcccgcctta ctccacgggc gagaccggca
     8641 ggctaggcag ccccagacgg cgtgagatcg gtcacggcgc cctcgccgag cgggcgctac
     8701 ttcctgtcgt gccatccgag gagaccttcc cctacgcgct acgcataata tctgaggtgc
     8761 ttgagtcgaa cggctcctcg tctatggcga gcgtgtgcgg ttcgacgctg gcgctcatgg
     8821 acggcggcgt gccgatcaag gcccccgtgg ccggggttgc tatgggtctc gtcaaggagg
     8881 gcgaagatta cgtcatcctg acggacatcc agggtctcga agaccacatg ggtgacatgg
     8941 acttcaaggt cgccggcacg agcgacggca tcaccgcgct gcagatggac atgaagatca
     9001 cgggcgtctc cgccgacctg ttgaaggagg ctctcgggca ggcccgcacg ggacggctcc
     9061 agatcctgga gacgatgcgc gaggccatag ccgagccgcg cccggaggtc tcggacttcg
     9121 cgccgcgggt cgaggtattg aagatcccca aggacaagat cgggatgctc atcgggccgg
     9181 gcggcaagac cattaatgcc ctgcaggagc agttcggcgt gaacatctcc gtcgaggacg
     9241 acgggacggt gttcgtggcc ggcgagggcg tcgtctccaa ggaggcggcc tcggcgataa
     9301 acggtatgat gaaggacgtg gaggccggcg agatctacac cggcaaggtc gtcaagacca
     9361 ccaacttcgg ggccttcgtt gagcttacgc cgggccgcga cggcctggtc cacatctcgc
     9421 gcctcgcgcc gggcaagcag cgcgtcgaga aggtcgagga cgtggtgacg gagggcgacc
     9481 gcatcaaggt gcgcgtcctg gagatagaca agcagaaccg catctcgcta gagaagctgg
     9541 aggactagac cttgagcgac ccgaacattc ggcagaagca gcttccgggc gggttgcggg
     9601 tcttcacgga gccgctggac gaagcgacga gcgtctcttt gggcgtctgg atccgggccg
     9661 gctcccgcga cgagaaggac gaggtcgcgg gcatcagcca cctgatggag cacatgctct
     9721 tcaagggcac gccctcgatg aacgccctcc aggtcgccga ggcgttcgag agcatcggcg
     9781 cccaggagaa cgcggctacg ggcgaggagt acaccgtgct ctacgcccgg ttcctgcccg
     9841 agcacctcga aaaggctctg gacatcatga gcgacatggt gctccggccg acgctcgcgg
     9901 acctggagcg ggagagggag gtgatcgtcg aggagatcaa gatgtacgag gaccggccgg
     9961 accagttggc cgatgagtac ctctcgtccc tgatcttcca cgacgacacc ctcggacgcc
    10021 cgatcatcgg ctccgccgag accgtccgcg gcgtggacca cgatacccta cagaggttcc
    10081 acggctcgac ctacaacgcc tctaacgtct tcgtcgtcgg cgcgggcaag ctcgaccagg
    10141 acgagttcga gcggatggtc gaggagaagc tctccggtct gccggagggc gagccgttcg
    10201 tccgcgaggc agatccgaag cagcccgaga gccgcttttt ctacaagttc aaggagaccg
    10261 agcagtacca cgtctcgctg ggctcgctag gcatcccggc gaagagcgag gaccgctatg
    10321 cgatgtcggc tctgaacaac gtgctcggcg gcggcatgag cagccggctc ttccaggaag
    10381 tgcgcgagaa gcgcgggctg gcctacgccg tctactccta ccaccagggg tactcggata
    10441 ccggagcgat caagacctac gtcggctcca cgaccggcaa cgtcgaggaa gcggtgaagg
    10501 tcatcaccga gcaactggag aagatacagc acgagctggt tactgacgag gagctggagc
    10561 gcacgaagca gcagctcaag agctcgaccc tgctcgcgct ggagagcacc gctgtcagga
    10621 tgacccgcat cggacgcggc atcataactg gctcggagct gctgaccccg gaggagatct
    10681 ccgacctcat agaagcggtg acgccgcagg atatagaacg gctcgccaaa aagcatctga
    10741 aactagacaa catgtacctc tccgcggtcg gaccgaagga gctcgacctc gggcagtacc
    10801 tgagcaagaa ctagccgttg gctggcgccg atcagacctc gggaaaagag atcgaggcct
    10861 gatctccggt agctgatagc tcctgaagga ggtaatccac gctgttagac aacaagacct
    10921 tgcaggtcat tccccttgga ggacttgggg agatcggcaa gaatatgacc gccgtgcgcc
    10981 aggagagcgg tattgtcctg gtcgacgcgg gtatggcgtt cccggatgag gagatgcccg
    11041 gcatcgacct cgtcctgccg gacttctcct acctgcgcga gcacgccgac gaactgaagg
    11101 gcatagtact gacccacggc cacgaggacc acgtgggagc cgtgccgtac ctgctccggg
    11161 agttcaacgt cccggtatac gcaacaaagc tcaccctggg gctgctcagg agcaagctgc
    11221 aggagttcgg catcaagaaa ggcgacctgc gcgagatcaa ggccggtgat acccagaacc
    11281 tgggcggctt cggcatggag ttcatcaacg tgaaccactc catcccggac gctgtcgcca
    11341 tcgccatccg cacgagagcc ggcctgatcg tcttctccgg cgactacaag atcgacctga
    11401 ccccgacgga gggtgatcct atggacctcg cgcgcctcgc ggcgctcggc accgagggcg
    11461 tcctggcgta ctttggggac tcgaccaact ccgaacggcc gggatacgta ccgtcggagc
    11521 gcgtggtcag cgacacgctc aaccgggtct tcgaggatgc ggatggcagg atcatcgtcg
    11581 cttcattcgc ctcgcatatc caccgggtcc agcaggtcgt cgaggctgcc aagcgccacg
    11641 gacgtctcgt cggtgtgacg ggacgctcca tgatccgcaa cgtagacatc gcccgcgagc
    11701 tcggctacct cgacctgccc caggcgatgt acgtgaacca gcgggacgtc aacaagatcc
    11761 cggactcgga catcgtggtc ctgacgaccg gctcgcaggg cgaaccgctg gccgcgctct
    11821 cgcgcatcgc caacggcacc caccgcacca tcgaggtcgg cgaggacgac acggtcgtga
    11881 tagcggccca tccgatcccc ggtaacgagc gcggcgtctc gcgcaccata aacaacctca
    11941 tgaagcgcgg cgcgaatgtc tgctacagtc cgctgatgcc ggtccacgtc tcgggccacg
    12001 gggcgaagga ggagcagaaa atagtcctct cgctcctgaa gccgcgctac ctggtccccg
    12061 tgcatggcga gtttcgtcac ttgcgccacc acgccaacac cggcatgtcc atgggcatcc
    12121 cggaggagaa tgtcttcatc ctggagaacg gcgcgggcgt cgagtttaac aacggcaacg
    12181 cgaagcggat gaagcccgtc gaggccggca tgttcctggt ggacggcggc ggtctggccg
    12241 acgactcgga gagcgtcatc cgggagcgcc agcagctcgc cgaggacggg atgttcatcg
    12301 tcgtggcccg cataaacggt cagaccggac aactgctggg accaccggac gtaatatcgc
    12361 gcggcttcgc gaacccacag ggttcgaacg gtctggtcga ggaatctata aatgtcgtgg
    12421 gcaagacgct cgaacgcacc gcgaagcagc gtctgaccga ctggagcgag ctgaagagcg
    12481 cgatccgcaa ggacctctcc ggtttcctct cccaacgcac ccgcaagcgg ccgatcatcc
    12541 tgccgctaat agttgaggtc taaacttcaa cctctactat atggtgcggt tgaccgtgag
    12601 caagaggttg atgcgctaga ctaaggctca tggcaaagag cagacgcgcg cccaagaggt
    12661 ccatgacaaa ggctccggcg aagaagcatc gcggaagcaa gaacgccagg aaaggtatgg
    12721 tttcgcgttt ctgggagcgc gtccggggtt ggttagcgcc ggaggtgatc ggcggggtgt
    12781 tgctcgcggt cgggttgttc tttactgcgg cgttcgtgag cgggcgaggg gcgtggctcg
    12841 gggatacggg gttgatggcc gcgactcacc tcatgggtgt cgcgggttac gcgctcgctc
    12901 cgctcggggc gctggccggc ggtttgttgc tgctgcagag gcttccggcc gtcaggacgt
    12961 tcgggggagg cttgatcttg ctgggcttcg ccacgagcat ggccgcgcgg ctgcccgagg
    13021 gacggctatt cgaggcgggg gaatatgatg cggcgggcgg tatcgtgggc agcgcgatct
    13081 acggcgccgt ctattggact ggtggagtag taggagccgc cgtggcgctg gtgtttctgt
    13141 acgctgtcgg tctctcctta ctcacgggaa tatccttcga gtcggcgctg ggcgcatccc
    13201 gtaccttcct gacttcatcc ctggacagtc ttcgagagtg gcggaagcgc agggctgagg
    13261 ccgccgaaaa ggccgccgcg gagcaaagga agtcgatcga gattccttcc cgatcatcga
    13321 gagcaaaaaa ggctccgaag ccggcgcaag gtgtagtgga cctctatccg gagctcgata
    13381 aggaggagtc ggctaaaagg gcggtcccgg agagtcagga gagcttcgag gtggtgttgc
    13441 ccgaggagcg gcaagagccc gccggcgagc cggtgatcca ccaggagagt gaaggaagcg
    13501 cgtcgggaga gtacaccccg ccgccgatgt ctttgctgaa attgggcggc ggaggaccgc
    13561 accatgacgc ggaggggacg agccagcgtc tcacgcaggc gctcgcggac ctcggcgtcg
    13621 cggcccaggt ggtccgggcg gttgtcggcc cgcgcgtcac gcgctacgag ctgcggctcg
    13681 gaagcggtgt caaggtcggg aaggtcagca acctccagca ggatatcgcc tacgctctgg
    13741 cggcgacgga ggtaaggata ctcgccccga tccccggcaa gagcgccgtg ggtgtggagg
    13801 tgccgaacac ccgaccggcg accgtgacgc tcggggacgt gttccaggac tatccggagg
    13861 gcaacgactg ggcgctgccg gtcgggctcg ggaaggatat ctccggccgg gcggtcttcc
    13921 tggacctggc tgatatgccg cacctgctcg tcgccggcac tacgggtagc ggaaagagcg
    13981 tcatgctcaa cgggctgctc acctcgctgc tcttggcgac ggacccgcgc gaggtcaaga
    14041 tggtcctcat agaccccaag cgggtcgagc tgtcgcagtt cgcgcgcata ccgcacctca
    14101 taacccccgt cgtcacggac gtgaagaagg ctgcaaacgc cctgggctgg gccgtcgcgg
    14161 agatggagcg gcgctatgaa gtcctggagc agaaaggtgt ccgatctctg gaggggtaca
    14221 acgcgcgcgc cgagtatccc atgccatacg tactcgtcgt gatagacgag ctggcggacc
    14281 tgatgatgca ggccggtgcc aaggtcgagg acgccatcgt gcggctggcc cagaaggcgc
    14341 gggcggtcgg aatacatcta gtggtcgcca cccagcggcc gagcgttgac gtgatcacgg
    14401 gcatgatcaa ggcgaacatt ccgagccgca tcgcgttcgc cgtatcgagc caggtggact
    14461 cgcgcgtgat cctggacacc tccggcgccg aggcccttct cggtatgggc gacatgctct
    14521 acaagccagt ctccgcggcg cgggcgtccc gggtgcaggg agcgtttatc tcggaggccg
    14581 aggtcgagcg agtggtctcg gcgagcgtgg cggcggcgcg gggcaaggcc gagaccgagt
    14641 tcgtcgagga ggtgaccgcg cccaagaacg agaagcagga gaccggagag cccgaggacg
    14701 agttgctgcc ggaggcggcg agcttcgtgg tggccaccca gcaggcgtcc gtgagcgcgg
    14761 tgcagcgccg gttccgggtc gggtactcgc gggcggggcg tatcatcgac gcgctggagc
    14821 ggaagggcat cgtcgggccg tatgaagggt caaagagccg ctcggtggtg gcgagcgagc
    14881 tggagttgag cagcatattc agcggggaat cgagtgacgg cggcggctcc ggcggaccct
    14941 ccgggacagg ggagaaacag tccgagagct ggtagctttt gtgataggct aatcaatggc
    15001 atgaatgacg aaggccagag attgtacgag cgcgacggcg gactggagat cgggcccacg
    15061 ctggagaaag cgcgtcttca gcgcgagctt accctcgacg aggtggagca ggcgaccaag
    15121 atccgcaagc gctacatcca ggctatcgag aaggatgatt acggggtctt gcccgatgcc
    15181 gtctatgttc agggtttcgt aaagacttac gcgaattacc tgggtctaaa cggagaggag
    15241 ttggccggtg aactcaggag ccgccgccag tcccgccggg agcggcacgt aaaccatgcg
    15301 aggttgacca agggcggctt cgacaaaccg ctcataaatc ccggcggggt cgcgggagcg
    15361 gagaagcgcc ggatctccgg cggcgcggtg ctcgggttgc tggcttcgat cttgttgatc
    15421 gtgctggtta tcggggcgtt gtattacgtt ggccggggct ccagagtcca ggtgccggat
    15481 gatggcgcgt ccgctccgca ggctgagcag gccgacaaga actccgataa acccgagagc
    15541 gggaagccgg atgcgggagt gtcttccaca ggttccgctg gcaccgcagg cggtactaag
    15601 aaggcgggag gcaacgaggc cggaaatgcc ggagccgaga ccccggctcc ggtagaggtc
    15661 gcgatacagg tggaggggtc tcctgcctgg atcagcgtca agtccgacgg aacggtggtc
    15721 tacgagcagg tggcggatcc gggtttctcg gataccttcg agggagagca gacggtcggt
    15781 atctcgacgg gcaacgccgg cgcggttaag ctcaaggtca acggcaagga cgaaggggcg
    15841 atgggcggtg ccggcgaagt cgtaagccgg gagttcacgc cggaatccga gagctagagc
    15901 tggaggcggg acctctctgc gaacaaaagg ggttggggcg cggtccggct acagtataat
    15961 agctgcaatg tatgcgagtc tttagcgacg atctccgtgg aggatagagt tggataagag
    16021 caaagccata ggcgccgccg taacgcagat cgagcgtcag ttcggcaagg gttccatcat
    16081 gcgtatgggc gaccgcggta cgcagaagat acccgtgatc ccgaccggcg ccatgtctct
    16141 ggacctggcg ctcggggtcg gcgggttgcc gaggggcagg atcatcgaga tcttcggccc
    16201 cgagtcgagc ggcaagacta ccctggccct gcacgtgatc tccgaggccc agaaggccgg
    16261 cggtctggcc gccttcatag acgcggagca tgccctggat cccgcctacg ccgagagaat
    16321 aggcgtggac cttgagaacc tctacttctc ccagccggat aatggagagc aggcgctcga
    16381 aattgcggac accctcgttc ggtccggagc cctcgacctg gtcgtcatag actccgttgc
    16441 cgctctcgtg cctcgtgctg agatagaggg tgagatgggc gactctcacg tgggacttca
    16501 ggctcgtctg atgagtcagg cgctacggaa gctctccggg tctctgaacc gctcggggac
    16561 cacggcgatc ttcatcaacc agttgcgcga gaagatcggg gtgatgttcg gttcgccgga
    16621 gacgacgccg ggcgggcggg cgttgaagtt ctattccagc gtccggctgg acatccggcg
    16681 catcggggcg ctgaagaacg gcaacgacac ggtcggcaac cagacgaagg tgaaggtcgt
    16741 caagaacaag gttgcgccgc cgttcaaggt ggtggagttc gacatcatgt acggcgaggg
    16801 gatctccaag gaaggcagtc tcctggacat cgggatcgag cagggcgtgg tccagaagtc
    16861 cggggcgtgg ttcggttacg gcgaggagcg tctcggtcag ggccgggaga acgccaagca
    16921 gttcctcaag gagaacgacg ctgtccggga gcgtatcgta cacgatatct acgataagct
    16981 cggcctcaac gccatagagg gcggcgagga gagcaatgga gagggcgtat ccccggagcg
    17041 gcccgccaag gagccggccg ggcaggtcct gtagggttcc ctggcaaaat atgccgcaga
    17101 taaccggcgt ccgggagcgc cggggaaggg ccagagtctt cgtcgatgga gagttctggg
    17161 ccgagattga cgcgaacgtg gcgctggagc gcggtctcta cgagggggcc gcgctctctt
    17221 gcgatgagtt ggacgaggcg cgggttgcgg gggagaaggc tctggctatg agccgcgccc
    17281 tgaactttct ggcatatcgc tctcggtcag agaaagaggt gcgggatcgg ctcttccgct
    17341 acgggtatgg agcggagact gtcgatggcg tgctggagcg tcttgcggag ctgggatacc
    17401 tggacgacga gggatttgcg cggacggcgg cgcgtgagaa gtcccgcaag tacggccccc
    17461 ggagggtgtc cgccgacctg atgcgcggcg gcgtgagcgg cgagatcgcg cgcgccgcgg
    17521 tcgaggaagc attcgcggaa cattccgagc gcgaaatggc gcgcgagttg gcggcccggc
    17581 gctataataa gtccgaaagg tccgatgctc aggcgcgtcg ggtctacggc ttcctggtac
    17641 gccgtggctt ctcggccgat gtttgcgccg ggatcgcgcg agaataccgt ggaccggatg
    17701 cgtccgaagc cgacgtgtag atactgatta tgatccggtc gctgccgggg cggcggcctg
    17761 ttttggagga caatctgtga atgttctatt actggtcctg gggctggccg tcggacttct
    17821 gggcggtgct gctctgggtt actacgtctg caagtcacgt atggaggcta ggcaggcgga
    17881 ggcgcgctcc gatgcccggt cgctcataga agatgccaac cggcaggtag aggcgtcccg
    17941 ccgcgaggcg gagcttgccg cgaaggaagc cgcgttgaag gtcaaggacg aagccgaggc
    18001 cgagttgcgt tcccggcgcg cggagatcgc ccgcgttgag gaacgtctgc agaaccgcga
    18061 cgctactctg gaccgccgtg agagcgaact ggaccggcgg cggcagaagg tctccgagac
    18121 cgaggaggcc ctacagcggc gcgaagaaga cctcaaatcg cgtgaggccg agcagttacg
    18181 ggccctcgaa gagatctccg gtcttacccg cgccgaggcc gaacgacgcc tctactccgg
    18241 gcttgagcag gacctcgagg accggctcgg ccgcatggtg cgcgaccgca ccctcgaagc
    18301 cgaggagcgg gcagataacg aggcgcgtcg gatcatctcg acgaccatgg agcgcctggc
    18361 ctcggacctc acctcggagt ccactgtcaa ggcgatagcc ctcacctcgg acgacatgaa
    18421 gggcagaatc atcggccggg agggccgcaa catccgggcg ttcgaggccg cgacgggggt
    18481 ggacgtgatc atcgacgaca cgcccgagac ggtggttatc tcctgcttcg acccggtgcg
    18541 gcgcgagata gcgcgcgtct cgatggagcg gctggttcag gacgggcgta tccatccggg
    18601 gcgcatcgag cagatcgtgg cgaaggctaa gaaggacgtc gagaaggaga tgaaggccgc
    18661 gggccgccag gctctctacg acgccaagat ctccggcggt atgaacaacg acctgatccg
    18721 cctgctgggc gccctgaagt accgtacctc ttacggccag aacgtactcg cgcactcggt
    18781 cgaggtcgcc aacctgtgcg gcatgatggc cgccgagctt ggcgctaacg tgaagatctc
    18841 tcgccgcggc gcgctcctgc acgacgtggg caaggctata gaccatgaag tcgaggggac
    18901 ccacgccctt atcggggggc gctacgccaa gaagagcggc gagtccgagg acgtggtacg
    18961 ggcgatcagc gctcaccacc atgagataga gatggagacc gtcgaggacg tgctggtggc
    19021 gactgctgat gccgtgtccg ccgcccgtcc cggcgcccga cgcgagacga ccgagactta
    19081 tatagaacgc ctgagatctc tggaggacat cgcgcttgga catcggggcg tggacaaggc
    19141 gtatgctatc caggccggcc gggaggttcg cgtgatggtg cagccctcgg aggtggacga
    19201 ccggatagct gcgaagctgg ccttcgatat ctcgaagcag atagaggagg acctggagta
    19261 tccggggcag atcaaggtga ccgtcatccg tgagagccgg gttagcgagg tggcccggta
    19321 gccgaggggt accgcgaagg accgtaccgg gatctcggcg gcgggtttgc ccggctgact
    19381 ggtgtcgagg gcgcccggcg aggcccctca cgtatcgcct ccgtggaaga cgcgctggtg
    19441 gagttgctcc ggaactcccg cgacgccgga gctggcagcg tttacgtcgc ctctgtcttg
    19501 cgccgtcgcc gctaccggac tctgaccgtt attgatgacg gggagggcct ggatgaccgc
    19561 tacgcggaga tgatcttcga gccgggagtg accagccgcc acctctctcc gaccctggat
    19621 ccctcggacc ctggttctac acacggcgcg ggcctctccc tctaccatat ccgcactctg
    19681 gcattgagcg ccgcctgcgt ctcggcttcg tccccgacgt cgttctcggc gaccttcgac
    19741 actattacct tgcccgaacg tgtcctccaa tcccccagcc gagcatcgag gtccaatctc
    19801 ctcgccacta cagaagcctt tgctcactca ccccaggctc ctaccgtcta ctatggatcc
    19861 ccggccacca tcctcgctac cctcctgaaa taccgcataa tacaatcaaa tgaatctgcg
    19921 gccgggatag cagaggaggc gaggaggttg gggttgcgtg tctcgttacg gaccgtgcat
    19981 cggataaaga gaggcgagat cgaggctgag gcgccggttg gaaggtcgag ggatgcgcgt
    20041 gaacgcggga gtgttggagt cggtggggga agagccgagg gtgggggaga catactcagc
    20101 ctcggagaag aggaaatggc acggatcgcg gctatactag acgaggcggc gcgggcaagt
    20161 tacctggagg tacgcgacct ccggcagcat tcgcgtccgg gggagatcag gctgagcgcc
    20221 cgagtcgagg agccggagga tctatatgag cagtagaacc gaagccgagc aagctgtcgg
    20281 gcaagccgcc gggcaggccg tgagggtcgc gttgtcgacc aagcccgcaa aaggcgtacg
    20341 cccgggctca ggcgaagcca ggactgcctg tataaggacc ttcggctgcc agatgaacgt
    20401 acacgactcg gacaggatcc ggcgcatgat ccgtgacgcc gggtacgccg aggtccccac
    20461 atacgaagag gcggacctcg ttatccttaa cacctgctac gtccgggaga atgccgttga
    20521 ccgtgtccgg ggtcatctcg gagagctgac ccgtctgcgc cgcgaaggcc gcgtcaagag
    20581 gatagccctt accggctgca taggggcagc cgaggagtcc tctgaactaa agcgccagta
    20641 cggtattgac ctggtgttgg gaacgcataa tacatacgag ctggcggagt tcgtaggttt
    20701 acccacgatg gaggagacgt atacgccgga ccttcccggc acggagggtg acaactccgc
    20761 cttcgtcacc ataatgacgg gatgtaacta tcagtgtagc tactgcatag tgcctacggt
    20821 gaggggccgg atggtttgca ggccaatgga caacattctc gaggaagtgc ggaggcttgt
    20881 agagagcggg accagctaca taacgcttct gggtcagacg gtggacgcct ggcgggaaga
    20941 cggaagaagg ttctgcgacc tgctagagag ggtgtcggag atagcgccgc gcgtctggtt
    21001 cactaccagc catccctcca acatggaaga caggacactg cggctaatcg gcgagaagga
    21061 tacgatagta aagaggctcc accttccggt acagtctggc tccgatcgca tgctgcgggt
    21121 tatgcaccgc gggtacaagt cagacaggta ccggcgcaag atagagctat tccgggaagt
    21181 cgctccagag gggaccctga gcacagatct catcatcggg catccgggcg agacagagga
    21241 ggaccatcag gagacgctca agcttgtaga agactgttca tttgattccg cctatgtctt
    21301 taagttctcg ccccggcggg ggaccgaggc cgcgagcata caggagagcg tcccggatga
    21361 cgtggtccag cggcggtttc tccaggtgct aagaaagata gagcagaacg ccttcaaccg
    21421 taaccgcacc aggataggta gggtagaaga catctacata cggtacgagc ggaaccggga
    21481 tggcgtggct atcggagaga cctggaacgg gcacgcggta cacgttgaga cggatgcgcc
    21541 cacaggccag tacgtccgcg cggagatcaa ggccgccgga cctcacgtgc tttacgctgg
    21601 ggagactttg atctgaacag ggctatcgta gtctgcgggc ccacagcgag cggaaagagc
    21661 gacctcgcgg acgggattgc tcacaatctc tcggagagct atcgtaagga agtcaccacg
    21721 ctcgtggtcg attccatgca ggtgtaccgt gagataccca cgataaccaa ccagagacgc
    21781 cagcgacccg ccgagatggt cggtgtggct tccgtcctgg atgagtggac cgtcgcccgc
    21841 caccgggata tgagccttgg tatcctggcc ggcatcgata taccgtttgt cctggatgcc
    21901 ggtaccggta tgtacctgaa tgcccttctc ctggagatag aactctcccc caaggtcgaa
    21961 ccccacatcc gagccgtggc tgagtcccta tcacaggggg acgtgaaccc tcgccgggcc
    22021 agccgcgaga gagagctgcg actggccggc gcgccagaga gaagctccat ctgggacgcc
    22081 aacctgagat acgagaccac gctaatatat atccggcccg acagagagag cctcgacaaa
    22141 aacatccagc gtcgtgccaa ccacataata caaaatgcca ttgcggaatc cgagacgata
    22201 gcggctctga tctctcgcgg agcgaccttg agcccgcagg taagtggggc catcggcgtg
    22261 aaggagatgc tggatctcgt cgccggttcc ctgactgggg cagaggccga gtcaaggata
    22321 gcggcgcgca ctcgccggct ggcgcgacgg cagatccgat ggttcgataa gcttacccgt
    22381 actatctcgg atagagctgc tgtcgaagtg gtcagccaca gcagtgacgc agagacgatg
    22441 catactttgc atgatacaat aggctcatga gcgatacgga gaatacgggg atagacaggg
    22501 ccgtgcttgt gggcgcgggt gaggggcggt acctggacga attgggacgt ctggcgggta
    22561 ctctgggagt caaggtggta gccgaggtag agcagaaccg cagggatggt tctggttatc
    22621 tggggaaagg caagagggag gagttgcggg acgtcgtcgc cgaagccggg gctgcaatgg
    22681 ttatagcgga tgatgagttg acggcgtctc aggcgagggt tctcgagcgg tcgtcgggag
    22741 cgtctgtggt ggaccgcacg gagctgatca tcaggatatt cgaggctcat gccagggact
    22801 cggcgagccg gctggaagta gagcttgcag acttgcagta taggttgccg cgggtcaagg
    22861 ggcgaaacga agctcttagc aggctcggtg gcggaggcgt aacaacgcga ggaccgggcg
    22921 agcagcagct agagtacgac cggcgtgtaa tccgggagag gatgtccagt atagagcgca
    22981 agctcgagga ggagagatcc gctagagcgg tacagggttc gcggcttagg aagtccggtc
    23041 ctcctacggt ggctctggtg ggttatacga acgcgggcaa gactactata ctcaacagga
    23101 tcagcggcgc cgggagatcc actatggacc gtctgttcga gacgcttgag actacttcgc
    23161 ggcttgtcga gggtcgggca gacggcaagg tcgcgcgccc ggacttcgtt ttgacggata
    23221 cggttgggtt cataaggaag ctccccaagc aactggtgcg ctctttcgag agtaccatgg
    23281 aggtcgcgag agacgcggat gtgcgtgtgc tttgcgcgga tgcttccagc gcagagctcg
    23341 aagacgaaat cagcactgta agagagatcc tcgacgatga gaacgctgga gagagcggtg
    23401 tgaacaacat cctttgtctg aataagtggg atctcatctc taaagagagg gggtctgacc
    23461 tgcgagcgga gtacccggac gcggtaacga taagcgcccg gaatggcgaa acccatccgc
    23521 tcctggagcg gatttacgac tctatagccg gaggacggga gaggatgaac ctcctgatac
    23581 ctcactccga gtacgctaac gccgctcgtc tatatggcgt agccgagatc cacagctcca
    23641 atgacacaac tacgggcgtt tgtctcgacg tgacgcttcc caagcacgtc gccagaagat
    23701 acggccgcta cagagtagca taatacccat agccgtgacg gcagcgatca agatatatcc
    23761 tcctccttca gagacttttc gtgtagagga cctgtatacg gacattgagt ttcccgatca
    23821 caatataact gacccgtcgc ttccttatgt agtaatcaat atggtctcgt ctctagatgg
    23881 aaaggcggca cttaacgggg tggcgaacgg catcggagga cccgctgatc gcagggtcat
    23941 gcgcaacttg aggtccaggg ctgatgctgt actcaggggg gctggcactc tgcgggctga
    24001 gaagatctcc gctggcgtac ctgaggacct ggcccgccgt cgaatgtccg agggtcggcc
    24061 cgcgcaaccg ctggaactga tactgtcgag aagcgacaca gcatccttga gcaacctctt
    24121 gaacgcatct tcaggcaccg ttgcgcttgt gactcaggat cgcggacccg tcaagaatct
    24181 cgaagacatg gagcgtatag tcgtgccggc ccggccagat ggtctcgtgg atctggttaa
    24241 ggccatgatc atcctgaagc gggaccacgg cttgagcgtc atcctcctcg aaggcggtcc
    24301 gtccctgaac cacgcgctta tctcttcagg actcgtgagt gagctgttcc tgacgttatc
    24361 tcacaggctg ctcggcggca gccaggatca aatcctcacc ctcctgaatg gccagccact
    24421 tccgcccgaa ctatcaactc cgctccgact catatcggca cacctcacag acgacgaatg
    24481 gttcctgcga tataagatgt aatgagatat ccagggaaaa agacgccgag tttccgataa
    24541 gtacaattct cggaaactgt accacaggct gtctgggtgc gcttcttggg cgggtgtccg
    24601 tagcggagta tctacattta gtgctcaatt ggtggagagt cgtctatctg gttggtggag
    24661 ctttttgtag gcttttagct ttgactgttc agtaccggga tatctatact gctcttatgc
    24721 tggtgtttgt tattgtggac agtgtggttt gaaggaggtc ttttgggtgg tctgagtctt
    24781 cccgttaaac ttgcgcttgt ctgtctggtt ctggcgtgtg gtttcactgc ccgtgtggga
    24841 tgggcgtatc tggatcttgg atcctctgtg aaagagataa aagcggctca agctgcaagc
    24901 gacactcaga ctaccgaatt cgcccagagc gactcatcag gtagcgatat aaacgttaca
    24961 gcccaggata gcagttcgag tggtcaagag gctaccaatc aagagacgac caacgccgct
    25021 caggggcaat acgatgacgg ctccggcacg ggatcctcta gcctgatgtc ggcaggcggc
    25081 tcgaaggacg gacctgttcc tctgatgtcc gacgggacgt gccctatcga gtaccccctg
    25141 gaacttccag atggatgcta tcgtattccc tagcttgctc ctaacctcct aaattatagt
    25201 ccttagaacg taacgaataa tttaggtagt tcctagacag agccctagat aggaagagta
    25261 ttatattgat atgccttgct tgcgagttct tcggcgcggc gtatgtagcg cattgtagtc
    25321 ttggggtctg agtgtccgag gtactgttgg atcaccagta ggggcgcttc attgacggcc
    25381 atgtttgtac cgacggtgtg ccggatcgag tgtggcgata ttgcttttgt aatacccgcg
    25441 gagttgacgt atttcttgac tatgtagcga attgacctcg ttgtgagtga tcggtccttg
    25501 ccttctcttg agatgaatag aggcttgcgc gcatcggcgt gagatgtaaa gctacggccc
    25561 gatagaagga cgaagcgctg gacgagttgc cagagctccg gggatattgg gacgttccgg
    25621 atcttgtctc ccttccctaa gatcctgagc agtacctgtc ccccacctac atcccggaaa
    25681 tcgcctacct tgataccaac cacctcggcc tcgcgcagac cacagctcgc cattaccgcc
    25741 agcattacgt aatcccgata actcgttgct cgcgccgcac tgagcatacg agccaactca
    25801 tcctcggtca ggacattgta ggagggatcc tgcctcaccc gcgggctttt cgcgaagaac
    25861 cgtagcgcct ccgggttgac gctcgtcgct cctcccatgt acgtaaagac caggaacctt
    25921 cgcagagcag cgagcttctt cgccgtagtc gccgacgcat aagtcttgag aagatgctcc
    25981 cggtacagag agatatcctc cgccgttatt tgctcaagat cctttccgat ctctcttgtg
    26041 tacgccagga acatcctaat ctctgtatta taagttttta tagtctgcgg gcttgtgagg
    26101 gtacggagat agacggagag cagccgctcc tctgtggacg ttggtgagag ggtggctacc
    26161 tcggattcga gaggtgcggg gaggttagtc ttccgggtcg tcataggtga tcagggagac
    26221 aacgtcatgc ggagccaggc gctcccggcc gttgaggtat gaaagttcga tcaggaatgc
    26281 ggcgccggcg accactcccc cggcgctctc gactagctcg cacatcgcct gcgcggtgcc
    26341 gccggtcgcg aggaggtcgt ccatcaccaa gacgcgggtg ccgggctgta tcgagtcaag
    26401 atgcgcctcc agagtctcga agccgtactc aagctcgtag gaagccgcga tagtctcgcg
    26461 cgggagcttg ttgggtttgc gcgccaggat cagtccctta cccgcccggt acgcgagggc
    26521 ggtgccaaag acgaagccgc gcgcctccgc cgagactatc cggtcgtagt cgatatcctc
    26581 ggtagcgctc gcgagagagt caacagccgc gcggagtgcg ggaccatcct ctatcagggg
    26641 cgtaatgtct ttaaaggaga taccgggctt cgggaaatcc gggacggtgc ggatatggcg
    26701 ttcgatctcc cggacgtcga ttccggatgc cactagggga cgaagctctc gatgatgctc
    26761 gccatcatca gttccttaag gcgagaatca accgcggcca ggtggcgggc cttgttcatc
    26821 gcctccgcca cgcgaacctc gtcgctggag cgcaactcgg ggttcagaat ctgcttgaac
    26881 atcgcagcct cgcggtccac agcggccatg ataccgcgca gatgacgagg ctggatcgag
    26941 tacttcgaca tctccagcgc catccgggcg atctcgacgt ctcgctgtgt caactccccg
    27001 gtccggccga tgacgccgac ggagatcagg tcgtccacaa agcgggagtc ggcctccagg
    27061 gcgtccgcca gctcttctct agagtagaca cggtcctcca ggacgcggga caggtcgccg
    27121 ccgccctcgc cggtggatgg gaccggagcg ccgccctcga tacgcttctt tatcaccttt
    27181 agcggtagat actctttgtc ctgaagggtg aggatatagc gcagaagctc cacgtcctgg
    27241 tttgagaaaa ggcggtagcc acctcgggtg cgggacaggt ttatgaggcg ccgccgctcc
    27301 agatagcgga tcttgctgac cgaaagcgtc ggaaagtcct gccgcagccg ggttactacc
    27361 tcgcctatgg tgtagctgtc cttgttgccg ccattctcca cgctaactca ctcccatcct
    27421 ctacgccatc acaccggccc accaatagac gctctacgta ttgacgaact tgaaccggta
    27481 tttgccgacc tggatctcat ccccattcct aaggtcaacc gagtcgacac gcacgcggtt
    27541 gacgtaagtc ccgttcaagc tcccggagtc gctgatcctg aacccccgcg tgctctgtag
    27601 tatctcggcg tggttcctgg ataccgtcac atcgtccagg aagatattgc aactcggaga
    27661 gcgacccacg gtgacgactt cctccgagat gtcgtgcatc ctgcgtccgg cagtaccctc
    27721 tacctgatcg agctcgatga gcgccgcacc agccttcccg cccgcgctcg cgcccgatgt
    27781 gtcctcgtcc tcgatgaact ctggcgaata ggagaccgta gactgaccgt gcgcgagctt
    27841 ggcgccgcac tcggcgcaga acttcatccg ctcgttgacg ggagacccgc actctggaca
    27901 gacgttcatg ccggaccccc tccctgctct tcttgtgcgc cgccggtgcg gtccatcaga
    27961 acccgcgcca actcctgggg cgagagcgcc gcatcccccc ggcgcaccag ctcggcccgg
    28021 atcaggtcta tccttccctg caatatacga cgccggtagc tgatctcccg ctcttccccg
    28081 gtgagcgcct ccagacgccc gcgcaactct tcctcggaga gcgaggtgac gtcatctaca
    28141 tcttcccatt caaatccttc ggaccccacc catgtactca taagcgaaag tataaactca
    28201 tctcaccctt aagtacagtc ttagatttgg ggtttttcac ttaagaaagg ttgagatgcg
    28261 aggtactcgt catgggatct cacctcggtc cgcgaggagg tagaaaccga cggcgtagag
    28321 tgccgcggcg gcgagagaga ggacgcatcc agcgtagaaa atcgtctctc ctagagcgcc
    28381 ggggctgaat atgacgatcc cgaccgagac cattagcacg gctgtagcag ctttcccgac
    28441 gacgttcacc cgcatcttgc cccttacgag gaagctgccg agcagcgcga gcacatccct
    28501 ccccacaagg atggcgacgg cccagacggg caggagtccc cggattgcga gcacgatggc
    28561 ggagctagag agcatcagcc ggtccgcaac ggggtcgagg agttgaccga gcctggtcgg
    28621 tccctgacgc ctcgctatgc ggccgtcgag gaagtcggta agggccgcgg cgacgaagac
    28681 cgcgacggcg atgccatccg cctcggcgag caaaagcccg accacgaccg ggaccatgag
    28741 aaggcggaag agggtaagag cgttcggcag cgtcatgacg cggtcgctcg gtgggttcct
    28801 gtcctcgact cttggcatgc cgctcctgaa tctagaccag atcttctgat ctaacggcta
    28861 cttcttacac gcaaccgcac gcttaccccg tagagatcat acctctcccg cagcgcgttc
    28921 tccaggtatc tgagataact atccggtata tcttttggcc ggttcgcgaa gagcatgaac
    28981 ctgggcgggg cgctaccggt ctgggtggcg taacggatct tgacgcctct cggcggcgcg
    29041 ctgcggtcca cgagccggtt tacgaggtcg gcgatctcgt gcgtcgggac ccgcaggttg
    29101 tacgcctgcc ggagccgggc ggcgaaggga aggacttctt taactccggc ccccgttagc
    29161 gcggagatgg agatcgcgtg cggcttgagg tcggtcatgc gcgcatagag gctgtcctct
    29221 atctcctgca agcgctcccg cggcacgagg tcgctcttgt ttataaggat gccacaggtc
    29281 ctcccggcct cctcgatcct gcgcgcgagc tggaggtctc cacccacgag gccctccacg
    29341 gcatcaacta acagcagcga gacgtccgag cgccggatcg cctcctccgt acgcagagac
    29401 gagtagtacg gcactccgtc ggccctcctg gcgctccggt tgacgcccgc cgtatcgagg
    29461 agcaggtact tctctatacc ttcggggctc tttacctcga cctctccggc cacggcatcc
    29521 gtggtagtac cggcaacgtc ggagactacc gcgcgctctg ctcccaaaag gcggtttagg
    29581 agcgtgctct tcccgacgtt gggcctgccg atgatggcga gacgcgggat cttgtcttcc
    29641 tcatcagcct cggtctccgg aaggcgctcg acgatggcgt ccaggagatc cccggtgccg
    29701 agcccgtggg ccgccgatac cgcatacggc tcgccctcgc ccagagcgtg gaacaggtga
    29761 cggtccgcat cgttggcggg gtcatccagc ttgttgacgg cgagcacgac gctaccgggg
    29821 gcgcggcgca actcgcgagc tatggcggcg tcggcctcgg tcggtccgag cctggagtct
    29881 gtgaggtgga ggatcacgtc cgcctcttcc gcggctatcc tcgcctgcag ggtcacaagg
    29941 gcctccagac cgactttcgc ggtgggctcc atcccgccgg tatctaccag cgtgaactct
    30001 ctaccggccc actcggcacg gttgtacgat cggtccctgg tcgtaccggg ctcaccggag
    30061 acgaccgcct gccggcgtcc caggatgcgg ttcaccagcg agctcttccc cacattagga
    30121 gccccgacga tcgcgaccac cggcaagcgg ctcacgagcc gtccccgact tccttcgcgt
    30181 gcttctccgg cagcgcgtag atggtacgca ggatctcgtc cgccgcctcg cggtaagccc
    30241 gtccacctct tgtagtatta tctgtagtca tcggttctcc gatgtaggcg tggagcttcg
    30301 cgccgcgcat ccgttgggcg aggttcggga tgcggctcat atagatggga aggacgggag
    30361 cgttgccacg ggttgagagc atgacgaccc cgctcttggc gtctctcgaa ataccgtggt
    30421 tgcggtgggt gccttccggg aagatcccga gcgtccagcc gtcggcgagg tagtctagcg
    30481 cggcgcgcag agccgcgcgc ccgccactct gacggtcaac gggaaaagtc ccgataaacc
    30541 tgaagaactt gccgaaaggg aagacgaaca gctctttctt acccatccat tgcacccgcc
    30601 ggggtaccgc catgttgacc aggacaggat ccgcatactg actgtggttg gaggcgagaa
    30661 ttagaggacc ggtctttggc acgttctccg ccccgtgtac cgtaaacccg aagaggaccc
    30721 tgccgatcaa gatcatcaat ctccggaaag cgcgatacct gaaagacatc ctggtagacg
    30781 tactcttcat cggagcacac cacgcaatct cctcataccc tgctcctggt ctgacgcgcc
    30841 aggtcgagga ccttcgagat tacaccgtcg agggttagtt cggtcgtatc gaggacgacg
    30901 gcgtcgggag cgggccgcag cggcgagacc tcccgctcgg agtcctggcg gtcgcgggtc
    30961 tctatcgcct tgcgaatgcg ctctagctcg ccctctctgc cgctctggac ggccctgcgc
    31021 cgggcgcgct cctcgggcgc ggcggagagg aagactttca ggtccgcgtc cggcaggacg
    31081 accgttccga tgtcccggcc ctcgacgacg gcgccgccct gctggcgcgc gcgctcggcg
    31141 gcccgccgct gcacggtgag aaggacctct cgtaactccg ggtgcgcgga gatcttggac
    31201 gccgccgcag agacctcggg cgtgcggagg tccgggtcct ggactctctc tccgtctatc
    31261 ctgacgccat cggggtccag gtcaaccgcg cgggccagac gcgcaagccc cgcgccgtca
    31321 tcgaaagcga cgccgtcgcg cagggccagg agagcgacgg cccggtaggc cgctccggtg
    31381 ttgaggttca cgacaccgag ccgctcggcc acggcgcgag cgacggagct cttcccgctt
    31441 ccagcgggac cgtcgatggt tacgaggagt cctcccattg atgctcgcag tatagccgat
    31501 agcgtgaggt ggttatcgca aggagtcgag acgctcgaaa tattcgggga aggtcttggt
    31561 gacgcatccg gggtcctgga tggtgatgcc cggcgcgacc aggccggtga cggcgaaggc
    31621 catcgcgatc ctgtggtccc cgtaggtcct gacttgcgcc ggtcgaaccg gtcccggaga
    31681 gatttcgagc ccgtctcgct cctcccgtac cttgacgccc atccgcgaga gctcggtggc
    31741 taccgccgag atacggtccg tctcctgatg gcgggtgtgc tcgatgttgg tgatgcgggt
    31801 cggtccctcg gccaacgggg ctatggctgc cagcgtgatc atggtgtccg agaactcgtt
    31861 catgtccacc tcgacgcccc gcagccgccc cgttccccgg acctcgacgt agtcccgcgt
    31921 catatccacc tcgcagccca tctcccgcag aacctcgaca aagcggaggt ccccctgcgt
    31981 ggaccccgat cccagccccg gcacccgaac ccttccgcca gccgcagccg ccgctgcaaa
    32041 gaagtacgaa gcccccgaag cgtccggctc tacgacgtac tcgccggctc ggtagtagcc
    32101 gggctctatc acataccgtc cgtcccgtga aacatccacc tgcacaccga aatctcgcat
    32161 tacgccggtc gttatcccga cgtacggacg agacaccaga tctcccccga cacgcaactc
    32221 aacccgcgcc ctcgcatacg gcgccgccat gagaagcccg ctgaggaact ggctgctctt
    32281 gctcccctca acccgcgcct cgccgcctcg aagaccgccg ccccgcacca caagagggaa
    32341 cctcccctcc tctcccccat accctacgtc agcccccaac tgcctcatgg cgtccaccag
    32401 gtcatccacc ggacgctctc tcatccgggc caccccatca acccgatatg gcccttctcc
    32461 caacgcaagc gccggcggca gaaaccgcgc caccgtcccc gcgttcccaa cgtatacgtc
    32521 catgcctttt cttggtatta tgccactttg tccggtaatg tgggctacgg ctcgggtgcg
    32581 atctacgttg acggcgaagc cgagttgggt gagggcggcg agtagccagt aggagtcgtc
    32641 gctgaagagt gggttgcgga tggtggatgc gccggaggcg agcgcggcga ctataagggc
    32701 gcggttggtg acgctcttgg agccgggaac cgggatctcg gcgtctacgg gaccgttcag
    32761 gggttttatc tctatctcct gcgggaagct gccccggacg tcctcgacgg agaagtcctc
    32821 tccggggact ccggatacgg gtctcactgg agagccttca attccgatga agtcagggaa
    32881 cggtgtcgtc caggaggcag gtctccaagt ctcacgggac cgatccgggt gcggtgcaga
    32941 gagatcagac ccagccccac cacagcgcag gctcgtcgta ttatgcggtt acggccttca
    33001 tggatcgtca gggtaatggt ggtgttgcgc gggccacggc gcacgctggt gatgacgggt
    33061 gggagcatct ttccgtcgtc gagcttgggg ccggcggcga gggcggcgag ggtcgcgtcg
    33121 gagacgggcg agtttacggt taggagatac tctttctcga cctcggagga cgggtgggag
    33181 atgcggtggg cgaacttgcc gtcgttggtt agcagtatca ggccagtcgt cgccgcgtcg
    33241 aggcgtccga caggcaccaa acccggaacc gacggcatca ggtcccggat ggtgcggcgg
    33301 tcccggccgg gctcgtcctt gagggtggtc aggtagccgg ccggcttgtg aagggcgagg
    33361 taggccgtcg tctgcggtag acgcacggcg cgcccgtcca gcaggatgct gtcggaagaa
    33421 gagacggtgg tcccgatggt cgcggtggag ccgttaacgg tcacgcggcc ggcggagatc
    33481 agggcttcgc ccttccggcg ggagggcgaa gctcccgcgc gcgccagata cgtctggagg
    33541 cgcaactacg attcctccgg ggtggccggc tcctgctcgc tcccggtctc ggcggccccg
    33601 acacgctccc ggacgcgggc cagctcttcg ggccccacga gggagtcgag cggcgcgaag
    33661 tcctcgcggg aggtggcgcc ggttgcggcc aggaactcat ccgtgacgtc caggagagcc
    33721 ggagcgccgg ggctctcccg gtccgagccg gtctcggcga gcaggccgcg ttcgatcaga
    33781 ccccggacca ccgcatcgga gttgacgccg cgggcggcgg agatctccgc ccgcgtcaac
    33841 ggccccaggt acagggcgca cgagagtacc tcgtgcgcgg cgttggagag cggagcgggc
    33901 cggctctcct cccggaaacg ctcgaccgcg gaggcgcaga ccgggttcgt ggcgagctgg
    33961 taaccgcccg cgacccgccg aagcacgatt cccgactcgc gcggcgagta ccgctcgccc
    34021 agcagcccca tcgcgtctcg cagttcggaa tcagagagcc cggcggcgga cttcagcgcg
    34081 gcctcggaga cgggacgcgg gctaaccaaa aggactgcct cgatgcgggc ggccggggta
    34141 gccggcgcgg gggtgggcgg ttcgctcaag ccggcgcttc cagtgtcagg gggccgagag
    34201 gctcggtctg tagcagccgc agccggcctt cgtgggccag cgacagggcc gcggcgaagg
    34261 ccacggcgga gtggagacgg tccatatccc ggacgaggac ctcgaaggag accgggccgc
    34321 gggcgaggga ggtccggatc agggccgcaa gctcctgcac cgtgacggtg atcggcccga
    34381 gatgccgcac cggcggctcc acgatccggg agagagcgcg gcgcgcggcg gcttcgagac
    34441 gctcggtccg tatcttcaac cttcccggac ggggctggac ggtctgggcg gtcgggtaga
    34501 agcccgcgtt ggcggcgaac ctctcgcgca gggactcggc tccgcgccgg atcctcaggt
    34561 acccggtgag cttctcggcg agctggtctg gagtgagggc ttcctcctcc gcctcgggct
    34621 cagcgtcgaa gatcggtgag agcgtccggc ttttcagcag cacgagcgag gccgcggagc
    34681 ttacgaaatc cgtgtcccgc tccagggagt gcgggcgctc cccggagccg tgcgaagtca
    34741 ggtacgtctc gaccagctcg cgaagcggga tctcgaatat ctccagctcg tccttcatga
    34801 tcagggcgag gagccactcg tagggaccgg agtacacgtc cagctcaacg gtgaagtcag
    34861 ctaacgagcg acccggagcg cccgcactct gcacggcaga cgggttaacc gctgaaggtt
    34921 tcaccgctga aggtttcacc gcacctccga gtgcaggggc aggaccgagg agagaggatg
    34981 atgctccgag ccgatggtgc gcgccagtgt gcggtccacg atccgcccct cggcgaccac
    35041 gaccccgcgc cggaggcccg tctcggcgtt gagcgcgtct ataagcccga tccccgccac
    35101 ctggcggatg gacgggagga gcgcgttcga gagcgctcgc gtggccgtca cgggcacggc
    35161 tgccgggacg ttcttcacgc agtagtggat gacgccggac tccacgaacg tgggatcgtc
    35221 cagggtcgtg ggccgggagg tctccacgca tcccccgtag tccacgtcaa cgtccacgat
    35281 cacgctgccc ggtttcatct cggcgaccgt ggcgcggtcg acgagggctg gcgtgcgcga
    35341 cccggccacg aaaaccgccc cgatcaggag gtccgcccgc aggacggcgt cctggatgct
    35401 catggcgctc gcggagagcg tagtcacgtt cctgagccgg gcgttctcca gaagccgcag
    35461 gcggtgcagg tcccggtcta taacagttat atcggcgccg aggccgctag ccatcctcgc
    35521 cgccccggac ccgactatgc cgcagcccag gatcacgacg cggcccggct tcacgcccac
    35581 ggacccgccg aggagtatcc cgcgcccgcc ggctggccgc tggaggtaat gggcgccgat
    35641 ctgcggaacg agccggccgg cgatctcgct catcggcgtc aatatgggga gacttccccg
    35701 gccgtcccgg atgctctcca tggcgaagcc cacgcaccgc gattcaacga gagcctgcgt
    35761 cagacccggg ttcacgggga gcgagaggaa ggagataagg attaaaccct cccgcagatt
    35821 ctgatgttct tcccgaatcg gcccgtacac cttcgctacg agctccgact ccgagtacac
    35881 tacagccggg tcacttacga gccgagcccc ggcctgctcg tactcggcat ccgaaatgct
    35941 cgaagcctcg ccggccccgg tttcgaccag gacgcgatgc ccggaccgcg ccagctcgtt
    36001 taccgcgtgg ggggcgagcg caacccggct ctcccccggc gtaagctcct ttactacccc
    36061 gatcctcact ccacgccttt cccgagagcg agactgacgg ccgccgccgc ggcgtcgaag
    36121 aatcccgcgt cgtcttcgta acttcgtccc atgctctcga aggttaaccc gaagcgctcc
    36181 tctaatccag cggcggtata cgcgacctcg acgcagtcgt gctgatcggt gagaccagct
    36241 accgaaatgc cggtcccgga cggcagcgca accctgcacg gcgcgagagt cgctctcagg
    36301 accgtcgtgg tgtggtgcga gaggccgcgg tgccgttcgc gggggtcggc ggcggagacc
    36361 ctgggcgcga gcaccggctc cgccccgaga gcgagcgccg cgttgagagc gatggcggag
    36421 gacatgcccc cgtgtccgta cggcgcgccg gtcccgatga ggcccggccc aatccccacg
    36481 atcacgacat ccgcaacggc cgcagcagcg agcagccccg aatacacgtt gggcgactcc
    36541 aggtcccccc cgaagcagtt tccacacgag attacgatgt ccagcagtcc cttatcgcgc
    36601 aagacgcggg cggtccggga gaactccacc ggcaaagcgc cgccctcctg ccacacgaag
    36661 gccgtccgga ctccgggacg caggtcggcc gccgcgcagc acgccggcgc gagatgagag
    36721 tgcagcggca gcaccacgac ccggacgccc tccagagtga gagcctgccg cgcaggggag
    36781 accggatact gaagcggcgt gtagggcatc ttgacgaaat ggtcctcatt acgcggcgct
    36841 tcgccgccgg ccgaaggcag tatcacggcg aagccgcccg ttccgaggtc catctccagc
    36901 ccgacggtat tcacgataac ttcgttacca ggtccgggag cagcccccat ctccgggtag
    36961 aagacgcccc tgtggacctc tcctgaaagc gggccgtcca agatctcgat gctgtacgag
    37021 aatcggtctt cctgtacctc cacgaccctc gcgcgccgga gtacgggacc ggttttcagg
    37081 ctatcccccg ctcgggaagc tcccactcgg gatgcgactc cacgaactcc ctcagctctc
    37141 tctccgcccc tcgcatacct agcggcacgc cgtcccccgg cgcgggcgcc atcatggggg
    37201 cgaagcacac cctgaatcct gcgccgtaga ggtctcgcgc cagagccgcc agcgtctccc
    37261 ccggcacgat gcgcggcggc aatccgtgga ccgctcgcac ctgaagcacc gcgtcggtca
    37321 gcgcggcgtg agcggctatc ctccgcaggg catcttcgag agccgctccg agagctggac
    37381 ccgttaaggc gattatctcc gcccgcaagg tccttgccgc gccttccggg tcttcggtcc
    37441 aggccgcgtt gcgcgcggcg gcgccgagaa ggccggtggg ccggggcatc tcagcggtag
    37501 agcgccattc catggtcgcc tccaactcga ccaacccggc ggtacgggtt gccagcgtcc
    37561 cggcgggctg gtcggtcggg aggatagcgg cccttcgggt agccgatgcc gtcgcggcgg
    37621 cgttcagcgg gccgcccgct cctctatgcc ggcctgctct agctcagaag tctttttcaa
    37681 gctctcgcca caggccccga cgaagccctt gaagagcggg tgcgggcgca ggggccg
//
LOCUS       DASSMB010000007        10880 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1812, whole genome shotgun sequence.
ACCESSION   DASSMB010000007 DASSMB010000000
VERSION     DASSMB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..975
                     /locus_tag="VFJ72_00600"
     CDS             256..975
                     /locus_tag="VFJ72_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7477990.1"
                     /translation="MKKVCIVGASGKLGKYMVQHALDRGYEVVGVCRTESVAKLDAFK
                     GRITIVPGATNDPEVIKRAVAGCDGVLVVLVPRGVHGYSTGTAQAVLDHAPPGARLVF
                     SCGWHITRDGQDVYSWKLKTLVKVFGPLARLARFADLDDQVEACRRVFASDTLWTVVR
                     GSDLEEGESQGLPVWSRHVGDPILASNLTRRVDFALFMVAALENDELIHEAPAIVGCQ
                     TPSAIAFKAQTQSLMEGVRQG"
     gene            complement(1060..2025)
                     /locus_tag="VFJ72_00605"
     CDS             complement(1060..2025)
                     /locus_tag="VFJ72_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7477991.1"
                     /translation="MDLQINSKSHIPVHVQLEEQLKHLILTGRFEVGSRLPSIRALAG
                     YLRVNRNTVARVISDLEREGFVESRRGSGVYVVEPPVDAERLRRQEVLEKVLDLAAAQ
                     GVRVEDLAYALLARAGAEPQVKTRILFVECTQVELDQFSAELEAQLPVEVDRVLLEDL
                     PERISGVEQLPWRVAVTTFFHVHEVQDLVEPLGIETVALLAEANLESLQRLTELPAGT
                     PVGVVGWGQTCMENLSRSIEGAGLDHLDFVSVNVDQEPEEVRTVLDQVRAVLCASVTA
                     QKLRERVTVPADVEIIEEDRILDKGGIEMLGGLLRQLPRTKQETE"
     gene            2121..2723
                     /locus_tag="VFJ72_00610"
     CDS             2121..2723
                     /locus_tag="VFJ72_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477992.1"
                     /translation="MREWSEFEMYRERSRDLLREAEEARLAREARNGKVEKRRRLPLR
                     EDERASIRVRWGCVEDEAGIADLLDLNGMPRWVAFEERFIVAVENGVVRAALRYRTES
                     KRLMLGLLVVDPWAGERRLARALYTGARDLGREMCVREVLAASGTRADYPGDAGYRRR
                     GKVWRWDATQPAGAGVSDVRGAHLSLTTLAGTFLRRVGWR"
     gene            2714..3331
                     /locus_tag="VFJ72_00615"
     CDS             2714..3331
                     /locus_tag="VFJ72_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7477993.1"
                     /translation="MAVIRLHDNLVSGNGYKVRLLLTQLGISFERIEYDIERGETRTP
                     EFLERINANGRVPVLETESGEFLPESNAILFYLAEGTPYLPEEKFERARVLQWMFFEQ
                     YSHEPNIAVARAWVHFGVEMTAERRAVLEQKRELGYAALGVMERRLADREFFAGERYS
                     IADIALYAYTHVADEGGFDLGPYPAVREWLEKCAAQPAHIPITAG"
     gene            3336..4109
                     /locus_tag="VFJ72_00620"
     CDS             3336..4109
                     /locus_tag="VFJ72_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET7477994.1"
                     /translation="MDAETRTNARIPRAPDTDGTTKGHESVSETAITFTLAGALAGAR
                     RCVPIAVSGFAIGLVFGTLAGQAGLGAVEATLMSALVFSGAAQFVVIGMWAASPPAGA
                     IALATLVVGLRHLLMGAALAPEFSRLPRRKAYTSVFFMADENWALTMGEFRKGRRDAA
                     FLLGGGMLMALSWTTSTLIGSTLGGALEDPSRWGLDFAFTAVFLALIAGMWKGRSDLL
                     PWTVAALVAVAAHHWLPGQWYILLGGLAGSLAGAMRRGA"
     gene            4099..4401
                     /locus_tag="VFJ72_00625"
     CDS             4099..4401
                     /locus_tag="VFJ72_00625"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET7477995.1"
                     /translation="MAPDPMTMLTILLMALATYATRAGGLWLTSRLKMSGRVEAWLNY
                     VPGAILISLVAPTVLAGGIADTLAAVAVLIVALRTGSLPAAMVTGVGAVLLFRTFL"
     gene            4475..5980
                     /locus_tag="VFJ72_00630"
     CDS             4475..5980
                     /locus_tag="VFJ72_00630"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HET7477996.1"
                     /translation="MIEDFESCYRVVRSRDARFDGWFFVAVKSTGIYCRPSCPAITPK
                     RRNVRFYPSAGAAQYAGFRACKRCRPDSVPGSPEWDTRADLAGRAMRLIADGVVDREG
                     VSGLARRLCYTERHLHRLLVAEVGAGPIALARARRAHTARLLIETTELPISEVAFAAG
                     FASIRQFNDTVRAVFSATPRELRRSRGRRGDVATGKISLRLPRREPFDAASILQFLGD
                     RAIAGVEEMFEGVYRRALRLPHGAGIVELSDGGDHVRCVLTLEEPRDLGAAVQRCRRL
                     LDLDADPVAVDDVLGADSILAPLVRRAPGLRVPGSVDGGELALRAVLGQQVSVAGART
                     LAGRLVQDVGQPLPEALTGAGERLTRLFPEIAAVAGAGLESLAMPAARRDALRSLAGA
                     LAAGDIALDPGAERDEARNKLLELRGIGPWTASYIAMRALGDPDAFLPTDLGVRRGIA
                     HLGHASDPKSVAALSERWRPWRSYAVQNLWASLEQRQRENEHRAENREVAS"
     gene            5977..6498
                     /locus_tag="VFJ72_00635"
     CDS             5977..6498
                     /locus_tag="VFJ72_00635"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7477997.1"
                     /translation="MNAYVDTVESPAGLLAFAVDEDGALIRLHFAESDQGAVMEEELE
                     AEGYDVASDESRTSGVRRELAEYAAGRRTTFDIPLSFAGSQWQKTVWRELTRIPFGET
                     RYYGEIADRLGRPGAARAVGRANATNVLPLVVPCHRVISADGTLGGFNGGLHLKTRLL
                     EHERRVLAAADAS"
     gene            6521..7447
                     /locus_tag="VFJ72_00640"
     CDS             6521..7447
                     /locus_tag="VFJ72_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7477998.1"
                     /translation="MSPKDLAALLLLGVLWGGSFLFIRVAVPALGPFALMEGRTALAA
                     AVLLLCAVVVGRLPKLRAYWRQFLILGAVNSAAPFTLIAFAQLELTASLAAILNSMTV
                     LFTALVAAFWTGEPLTTSKLAGVLIGVAGVAVLVGWDPVALSGTVIISIAAMLVASFF
                     YAVGSTYAKKTFAGRPPLEMAIGQQGGAALVLIVPALTTLPPVPLPGGAPSWTVALCL
                     LGLAVLSTAVAYLLYFRLLTNVGPTSTVTVTFLVPVFGLLFGVGLLGEPFGFGTLAGL
                     AIILVSVALVTDFRFGGAKRPDRDRAARDAAQ"
     gene            7486..7962
                     /locus_tag="VFJ72_00645"
     CDS             7486..7962
                     /locus_tag="VFJ72_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7477999.1"
                     /translation="MVDLMSLGEQVEKDFAVARRRARIHGLGRRLRGGKETRTLLPFD
                     RTRRTMGAVGGIRSGRIPVEISRIVGSAGKYDQFDDEFMPLRAASPERWKRIDRAFRS
                     GAELPPVSLYQMGGAYFVQDGHHRVSVARFHGAEWIDAEVTNFMTPQPLAAGAPAH"
     gene            complement(7975..8385)
                     /locus_tag="VFJ72_00650"
     CDS             complement(7975..8385)
                     /locus_tag="VFJ72_00650"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7478000.1"
                     /translation="MSILDELNKRGVGTLPGLVGLEILNAEEGRIHSHLELRDELMAP
                     NGYLHAATVVALADTSCGYGCFITLPEGAEGFTTIELKSNFLGTKREGVIECAANLVH
                     GGRTTQVWDASVSDEETGKPIALFRCTQMILYPR"
     gene            complement(8501..9082)
                     /locus_tag="VFJ72_00655"
     CDS             complement(8501..9082)
                     /locus_tag="VFJ72_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET7478001.1"
                     /translation="MRAKTLLGASLALMVALLTGTAWAQGSSQTLQLTPSHNSGVSGT
                     ATLTETGNGVEVKLDMQGLPKEGVKHLAHIHEGATCADDRAGNGGGVKFPLKDVVAQG
                     GAGSSTTNLDTSFDELFDGTPRYINVHAEQTDPKAVPPGVACADLKMTSADGSSSVPE
                     TGGTSPLTLLMAAGAALVALGAAAGFIARRRSV"
     gene            9190..>10880
                     /locus_tag="VFJ72_00660"
     CDS             9190..>10880
                     /locus_tag="VFJ72_00660"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET7478002.1"
                     /translation="MFRKTAARSLRAPRRVRAYLAANADRPVLLVASSEEEAERYARD
                     ARCFTDEPVVYLPSRDVVYGDVFDPPVSRVGERQRALHALGGAKLVVAGPLSLAERTP
                     LYDPMEISSGIEIELEAALERLVSLGYERVDRLSRPGEFAVRGGIVDVFSSTRRSPVR
                     VEWWGDEIESVRAVSLATQRVVRELEAVTLYAAREADLSSLAAANGEFPEDALRGVRV
                     PGLDRLLLDLDLTSPKDLLPAEAEAWFEEPREALPADLDGLVRELYDAEPPEADLRFV
                     GDEEGEVVSAPPVTAFAGSLREAARRLDSLVQDGLAVFVACGSGGEAKRAVYAFGEIN
                     RRVREAGRVDTSLPPGLYAVPGEIEESFTYPDAGIAVIRRDSLFGRKRARGGRPGRTF
                     TSFADLKTGDLVVHATQGIGRFEGLVAKEVLGATRDYMEVAYRGGDTLFVPYEQMELL
                     HKYIGGEGTRLDKLGGSTWAAVTDKVRNRVKALAGELLQLYARRAAAPGYAFPEDSEW
                     ERELEESFPYQETPDQEAAIAAIKADMQSPHPTDRLVCGDVGFGKTEVAVRAAFKA"
BASE COUNT         1696 a         3524 c         3767 g         1893 t
ORIGIN      
        1 ggcgggtctc tctcggcagg ctacaacgcc aaccttatcc agttcacctt catcggggag
       61 gtgttgctca tggtttggct cttgtggaaa ggggtcctgg cgaagaacga gttgcccgag
      121 tacgaaaggg agtgatccaa acgggcaaga cgcaacagat acgacctgat cccgtaagag
      181 cgaggtttca gaagagttac tgaagatgag aacggaagcg cagaccgcat acctaaagga
      241 cgcaaggaga tggatatgaa gaaggtttgt atcgtgggcg cctccgggaa gctcggtaag
      301 tacatggtgc agcacgcgct ggatcgaggc tacgaggtgg ttggcgtgtg ccggacggaa
      361 agcgtggcga agctcgatgc gttcaagggg cgcatcacca tagttccggg agcgacgaac
      421 gaccccgagg tcatcaagag ggcagtcgcc gggtgcgacg gggtgctcgt cgtgctggtc
      481 ccgcgaggcg tccacgggta ctcgacgggg acggcccagg cggtgctcga ccacgcgcct
      541 ccgggggcgc gactcgtgtt ctcgtgcggg tggcacatca cgcgcgacgg ccaggacgtg
      601 tactcgtgga agctcaagac gctggtgaag gtcttcggcc cgctcgcgcg cctcgctcgc
      661 ttcgccgacc tcgacgacca ggtggaggcg tgccggcggg tgttcgctag cgacacgcta
      721 tggaccgtcg tgcgcgggag cgatctcgaa gagggagaga gtcagggcct gcccgtgtgg
      781 agccggcacg tgggcgaccc catcctcgcg agcaacctca cgcgccgggt ggacttcgct
      841 ctcttcatgg tggcggccct agagaacgac gagcttatcc acgaggcccc ggcgattgta
      901 gggtgccaaa cgccctccgc gatcgctttc aaagcgcaga ctcagagctt gatggaagga
      961 gttcgccagg gttgatcgac gcaccgcctg attaggaacg tcatcccggt gcaataggct
     1021 cactcatcga aggagcacct tctggcttgc ggaccgccat tactccgtct cttgcttggt
     1081 ccgcggaagc tggcggagca ggccgccgag catctcgatg ccgcccttat ccaggatacg
     1141 gtcttcttcg atgatctcca cgtccgccgg caccgtgacg cgctcgcgca gcttctgggc
     1201 ggtgacgctt gcgcacaaca ccgcgcgtac ctgatccaga acggttcgga cctcttcggg
     1261 ttcctggtcc acattgacgg acacgaagtc caggtggtcg aggcccgcgc cctcgatgga
     1321 ccgagagagg ttctccatgc acgtctgtcc ccagcccacg accccgaccg gggtgccggc
     1381 cgggagctct gtcaggcgct gaagactctc caggttcgcc tctgcgagca gcgcgaccgt
     1441 ctctataccc aggggctcca cgaggtcctg tacctcgtga acgtggaaga aagtggtcac
     1501 cgctacccgc cagggcaact gctccacgcc ggagatcctc tccggaaggt cctccagcag
     1561 cacccggtcc acttcgaccg gcaactgcgc ctctagctcg gccgagaact gatccaactc
     1621 cacctgcgta cactcgacga acagtatccg cgtcttgacc tgcggctccg cgccggcgcg
     1681 agccaggagc gcgtacgcca gatcctccac cctaacgccc tgcgccgccg cgaggtccag
     1741 aaccttctcc agaacctcct gccgccgcaa tctctcggcg tccaccgggg gctccaccac
     1801 gtaaaccccg cttccgcgcc ggctctccac gaacccctct cgctccaggt ccgagatcac
     1861 gcgcgccacc gtgttccggt tcacccgcag ataccccgcg agcgcccgga tactcggcaa
     1921 ccggctcccc acctcgaacc gccccgtcaa tatgaggtgc ttgagctgct cctccaactg
     1981 tacgtggacc gggatgtgac tcttcgaatt tatctgtaaa tccataaatt atcctattgt
     2041 cctatgacga taagtcaatt ataattgtcc caggctgtta gggcaattat agcgctacgg
     2101 tgaagatgcg gaggcgagtg atgcgcgagt ggtcggaatt cgagatgtat cgggagcgaa
     2161 gccgggatct cctgcgggag gcggaggagg cgcggctggc gcgcgaggcg cggaatggaa
     2221 aggtggaaaa gaggcgcaga ttgccgcttc gggaggacga gcgcgcgagc atccgggtgc
     2281 ggtgggggtg tgtagaggat gaggccggga tcgcggacct gctggatctg aacgggatgc
     2341 cgcgctgggt cgccttcgag gaacggttca tcgtggcggt ggagaatggc gttgttcggg
     2401 ccgccctgcg ctaccggacg gagtcgaagc gccttatgct gggcctgctg gtggtagacc
     2461 cgtgggccgg agagcggcgt ctcgcgcggg cgctgtacac cggcgcgcgc gacctcggcc
     2521 gcgagatgtg tgttcgtgag gtactggccg ccagcggaac gcgagcggat tatcccggcg
     2581 acgccgggta tcggcgccgg ggcaaagtct ggcgatggga tgccacgcaa cccgctgggg
     2641 ccggagtctc tgacgtgcgc ggcgcgcacc tctccctgac aacgcttgca gggacgttct
     2701 tgcggcgcgt ggggtggcgg tgatccggct gcacgacaac ctcgtttcgg ggaacggcta
     2761 taaggtccgt ctgctcctta ctcagctcgg catctcgttc gagcggatcg agtacgacat
     2821 agagagggga gagacgcgca cgcccgagtt cctggaacgt atcaacgcga acggacgcgt
     2881 cccggtactg gagacggaga gcggagagtt cttgccggaa tctaacgcca tcctcttcta
     2941 cctcgccgaa ggcaccccgt atctaccgga ggagaaattc gagcgggcgc gggtcttgca
     3001 gtggatgttc ttcgagcagt acagtcacga gccgaacatc gccgtggccc gcgcctgggt
     3061 ccacttcgga gtggagatga ccgccgagcg gcgggccgtt ctggagcaga agcgcgagct
     3121 gggttacgcg gctctcggcg tgatggagcg gcgcctcgct gaccgcgagt tcttcgccgg
     3181 ggagcgctac tctatcgctg atatcgccct ctacgcctac acccatgtgg cggatgaagg
     3241 cggcttcgac ctcgggccct acccggcggt ccgtgagtgg ctcgaaaaat gcgcggcgca
     3301 gcccgcacac atccccataa cggcgggtta gagtgatgga tgccgagacg cgcaccaacg
     3361 ctcgtattcc tcgcgcaccg gatacggacg gcacgactaa aggacacgag tccgtatccg
     3421 agaccgctat tacttttacg ctcgccgggg cgctggccgg ggcgcggagg tgtgtaccga
     3481 tcgcggtgag cggcttcgcc atcgggctcg tcttcgggac gctggcgggt caggcggggc
     3541 tcggcgccgt cgaggccacc ctgatgagcg cgctggtctt ctcgggagcg gcgcagttcg
     3601 tggtgatcgg gatgtgggcc gcctcaccgc cggcgggggc catcgcgctc gccacgctcg
     3661 tcgtgggctt gaggcacctg ctcatgggcg ccgccctcgc gccggagttc tcgcggctgc
     3721 cacgcagaaa ggcgtacacg tcggtattct tcatggccga cgagaactgg gcgttgacga
     3781 tgggggagtt ccgcaaggga cgccgggacg ccgctttcct gctcggcggc ggtatgctaa
     3841 tggcgctctc gtggaccacc tccaccctga tcggcagcac tctcggcggc gctctggaag
     3901 acccttctcg ctggggactg gacttcgcgt tcacggcggt cttcctcgca ctgatcgccg
     3961 gtatgtggaa gggacgttcg gacctgctgc cctggaccgt cgccgcgctc gtcgcggtcg
     4021 cggcccatca ctggctgccg gggcagtggt acatcctgct cggcggactg gccgggagcc
     4081 tcgcgggagc gatgcgccgt ggcgcctgat cccatgacca tgctcaccat cctgctcatg
     4141 gctctcgcga cctacgccac ccgagccggc ggactctggc tgacgagccg cctcaagatg
     4201 tccgggcgtg tcgaggcgtg gctcaactac gtccccggcg cgatcctcat ctccctcgtc
     4261 gctccgaccg tccttgccgg cggtatcgcc gatactctcg ccgccgtcgc cgtcctgatc
     4321 gtcgccctgc gtaccggcag cctgccagcc gcaatggtca cgggcgtcgg cgcggtgctg
     4381 ctatttcgga cgttcttata ggaggttgga tgacgccacg gcgaagtccg atttccgcca
     4441 gtaattggtt gggtcaggtg cgacaatcct taccatgatc gaagatttcg agagttgcta
     4501 cagggttgta cggagccggg acgctcgctt cgacgggtgg ttcttcgtcg cggttaagtc
     4561 cactggtatc tactgccgtc cgagttgtcc ggccatcacc ccgaagcgcc ggaacgtcag
     4621 gttctatccc agcgccggcg ccgcccagta cgcgggattc cgggcctgca aacggtgccg
     4681 gcccgattcc gtgcccggct cgcccgagtg ggatacccgc gcggacctcg ccgggcgggc
     4741 catgcggctc atcgccgacg gcgtcgtgga ccgcgaaggc gtctccgggc tcgcgcgcag
     4801 gctctgctac acggagcggc acctgcatcg cctgctagtc gccgaggtgg gcgcaggacc
     4861 gatagctctc gcccgcgccc ggcgcgcgca taccgcgagg ctactcatcg agacgaccga
     4921 gcttccaatc tccgaggtcg ccttcgccgc gggtttcgcg agcatccgtc agttcaacga
     4981 cacggtccgc gccgtcttct ccgccacccc gcgagagttg cgacggtcgc ggggacgccg
     5041 cggcgacgtg gcaacgggga agatatcctt gcgtctgcca cgccgggagc cgtttgatgc
     5101 ggcttcgatc ctccagttcc tgggggaccg tgcgatagcg ggtgtggagg agatgttcga
     5161 gggcgtctac cggcgggcgc tgcggctgcc gcatggagcg ggcatcgtgg agctgtccga
     5221 cggtggggat cacgtccggt gcgtgctcac gttggaggaa ccgcgagacc tcggcgccgc
     5281 ggtgcagcga tgccgccggc tcctcgacct ggacgccgac ccggtcgcgg tagatgatgt
     5341 cctcggcgcg gattcgatcc tcgcgccgct cgtgcgccgc gcgccgggac tgcgtgttcc
     5401 gggaagcgtg gacggcggcg agctcgccct gcgggccgtg ctcggccagc aagtctccgt
     5461 cgccggagcc cggacactcg ccggccggtt agtccaggat gtggggcagc cgctaccgga
     5521 ggcgcttacc ggagcgggag agagattgac gcgcctcttc ccggagatag ccgcggtcgc
     5581 cggtgccggt ctggagagtc ttgccatgcc cgctgcccgc cgcgacgctc tgcgtagtct
     5641 ggccggtgcg ctggctgccg gagacatcgc gctcgatccc ggcgccgaac gcgatgaggc
     5701 ccggaacaag ctgctcgaac tgcggggtat cggtccatgg accgcgtcgt acatcgccat
     5761 gcgggcgctc ggagatccgg acgccttcct cccgaccgac cttggcgtgc ggcgcggcat
     5821 cgctcatctg ggtcatgcga gcgatccaaa gagcgtcgcg gcgctctcgg agcggtggcg
     5881 tccctggcgc tcctacgccg ttcagaacct gtgggcgagc ctggagcagc gtcagcgaga
     5941 gaatgaacat cgagcagaga acagagaggt ggcttcatga acgcatacgt cgacacggta
     6001 gagtccccgg cggggctact cgccttcgcg gtggacgaag acggcgccct cataaggcta
     6061 cacttcgccg agagcgatca aggcgcggtg atggaggagg agctggaggc cgaaggatac
     6121 gacgttgcaa gtgatgagtc ccggacgtcc ggtgtgcgga gggaactggc ggagtacgcg
     6181 gccggacgcc gcacgacatt cgacataccg ctatcgttcg ccggctcgca gtggcagaaa
     6241 accgtctggc gggagttgac gcgcatcccg ttcggcgaga ctcgctatta cggcgagatc
     6301 gcggaccgcc tcggccgtcc cggcgcggcg cgggccgtgg gccgggccaa cgccaccaac
     6361 gtcctgccgc tggtggtccc gtgccaccgc gtgatctccg ccgacgggac gctcggcggt
     6421 ttcaacggcg gcctccacct caagacccgg ctcctggagc acgagcggcg cgtgctggcc
     6481 gcggcggacg cttcgtgaga cagcgcccgg aggtcccggc ttgagcccga aggatctcgc
     6541 agcgctgcta ctgctagggg tgctgtgggg cggctcgttc ctgtttatcc gggtggccgt
     6601 cccggcgctc gggccgttcg cgctcatgga gggccggacg gcgctggcgg cggcggtcct
     6661 gctactgtgc gcagtcgtcg tgggacgatt gccgaagctg cgggcgtact ggaggcagtt
     6721 cctgatcctg ggggccgtaa actcggcggc gcccttcacc ctgatcgcgt tcgcgcaact
     6781 ggagctgacc gcctcgctcg cggccatact caactcgatg acggtgctgt tcaccgcgct
     6841 cgtagccgcc ttctggacgg gcgagccgct gaccaccagc aagctcgcgg gcgtcctcat
     6901 cggcgtcgcg ggcgtcgcgg tgctcgtggg ctgggacccg gtggcactga gcgggaccgt
     6961 catcatctcc atagccgcga tgctcgtcgc gtcctttttc tacgcggtgg gctcgaccta
     7021 cgccaagaaa accttcgcgg gacgcccgcc gctggagatg gcgataggcc agcagggggg
     7081 cgcggccctc gtgctgatcg tccccgctct gacgacgctt ccgcccgtcc cgctgcccgg
     7141 cggcgcgccc tcgtggacgg tggcgctgtg tttgctgggg ctcgccgtac tctcgacggc
     7201 ggtggcgtac ctcctctact tccggctcct gaccaacgtc ggcccgacga gtaccgtgac
     7261 cgtgaccttc ctcgtgccgg tcttcggcct cctcttcggc gtcgggctcc tcggggagcc
     7321 gttcgggttc gggacgctgg cggggctcgc gatcatcctc gtaagcgtag ccctcgttac
     7381 ggacttccgg ttcggtggcg cgaagagacc ggatcgagac cgcgcggccc gcgacgccgc
     7441 gcagtagaat ttatgtgatt aaagcttcaa gaagaggtaa cggcgatggt ggatctcatg
     7501 agcctggggg agcaggtgga gaaggatttc gccgtcgccc ggcgtagggc gcggatacac
     7561 gggctcgggc gaagactgcg gggcggcaag gagacccgca cgctcctgcc gttcgatcgg
     7621 acccgccgga ccatgggggc cgtaggcggc atccggtcgg gtagaatccc ggtggagatc
     7681 tcccgcatcg tcggaagcgc cggcaagtac gaccagttcg acgacgagtt tatgcccctt
     7741 cgcgcagcct cgcccgagag atggaagcgg atcgaccggg cgttccggtc gggcgccgag
     7801 ttgccgcccg tgagcctcta ccagatggga ggcgcgtact tcgtgcagga cggtcaccac
     7861 cgggtgagcg tggcccgttt ccacggcgcc gagtggatag acgccgaggt cacgaacttc
     7921 atgacgcctc aacccctggc ggccggagcg ccggcccact aacgcggact gacgctaacg
     7981 tgggtacagg atcatctgcg tgcagcggaa cagtgctatc ggcttgccgg tctcctcgtc
     8041 ggacaccgag gcgtcccaca cctgggtcgt gcgtccgccg tggacgaggt tcgccgcgca
     8101 ctcgatcaca ccctcccgct tcgtgccgag gaagttgctc tttagctcta tcgtcgtgaa
     8161 gccctccgcg ccttcgggga gtgttatgaa gcacccgtag ccgcaggaag tatcggcgag
     8221 ggcgacgacg gtggcggcat gcaggtagcc gttcggagcc atgagttcat cgcggagttc
     8281 gaggtggctg tggatcctgc cctcctccgc gttgaggatc tccaggccca cgagccccgg
     8341 tagagttcca acgccacgtt tgtttagttc gtcaagtatg gacaccgccg cgcctctccg
     8401 ttcgtgtgca gtagatcccg ccaattctct caaaactgac agtggcagag gggcgggacc
     8461 gcggccccgc ccctcgattc ccgcctatct ttggtgttgg ctagaccgaa cgtcgccgcg
     8521 cgatgaagcc ggcagcggct cccagtgcca ccagggccgc gccggccgcc atcagcagcg
     8581 tgagcgggga agtgcccccg gtctccggga cggagctgga accgtccgcg gaggtcatct
     8641 ttagatccgc gcaggccacg cccggaggga ctgccttggg gtcggtctgt tcggcgtgga
     8701 cgttgatgta gcgcggtgtt ccgtcgaaga gctcgtcgaa gctggtgtcg aggttcgtgg
     8761 tgctggagcc cgcgccgccc tgggccacga cgtccttcag cgggaacttg acgccgccgc
     8821 cattcccggc gcggtcgtcg gcgcaggtgg cgccctcgtg gatgtgcgcc aggtgtttca
     8881 cgccctcttt cggcagaccc tgcatgtcga gtttgacctc gacgccgttg ccggtctccg
     8941 ttagggtcgc cgtgccgctg acgccggagt tatgactcgg ggtgagctga agggtctggc
     9001 tggagccctg tgcccaggcg gtccccgtga gtagcgccac catcagcgcg agcgaagccc
     9061 ccagtagtgt cttggcgcgc atggatcttc ccctttcatc gtctttccct gtccagtctt
     9121 aaatacgtag ctgcgccgcc ggtggatgaa cgcactttga tcccggcgaa agccgggtag
     9181 aatccacgag tgttcaggaa gaccgcagcc cgctccctcc gcgccccgcg ccgcgtccgg
     9241 gcatacctcg ccgccaacgc cgatcggccc gtcttgctcg tagcctcctc ggaggaagag
     9301 gccgaacgtt acgcccgcga cgcccggtgc ttcactgatg agccggtggt ttacctgccg
     9361 tcgcgcgatg tcgtctacgg ggacgtcttc gatccgcccg tgagccgggt aggggagcgt
     9421 cagcgcgcgc tgcacgccct gggaggggcg aagttagtcg tggccggtcc actgtcgctc
     9481 gcggagcgga cgccgctgta cgatccgatg gagatctcct ccgggatcga gattgagttg
     9541 gaggcggcgc tggagcgact ggtctctcta ggttacgagc gggtggaccg gctctcgcgc
     9601 cccggcgagt tcgccgttcg cggcggtatc gtggacgtgt tctccagcac ccggcgctcg
     9661 cccgtgcgcg tggaatggtg gggggatgag atcgaaagcg tccgcgccgt ctctctggcg
     9721 acccagcgcg tcgtccgcga gctggaagcc gtaaccctct acgccgcccg cgaagcggac
     9781 ctctcaagcc tggccgcggc gaacggcgag ttcccggagg acgcgctccg gggcgtgcgc
     9841 gtgccgggcc tcgaccgcct gctcctcgac cttgacctca cgtccccgaa ggacttgctc
     9901 ccggccgagg ccgaagcctg gttcgaggag ccacgcgaag ccttgccggc ggacctggac
     9961 ggtctcgtgc gcgagctgta cgacgcggaa ccgccggagg ccgacctgcg gttcgtcggg
    10021 gacgaggagg gtgaggtcgt ctccgcgccg ccggtaacgg cgttcgccgg gtcgctgcgg
    10081 gaggcggctc gcaggctgga ctcgctggtg caagatgggc tcgcggtgtt cgtcgcgtgc
    10141 ggatcgggcg gcgaggccaa gcgcgccgtg tatgcgttcg gggagatcaa ccgcagggtc
    10201 cgggaggccg ggcgcgtgga tacgagcctc ccgccgggcc tctacgcggt gccgggcgag
    10261 atcgaggaga gcttcaccta cccggacgcc ggtatcgccg ttatccgccg ggactccctc
    10321 ttcggccgca agcgcgcccg cggcggacgt ccggggcgca cgttcacttc ctttgccgac
    10381 ctgaagaccg gcgacctcgt ggtgcacgcg acgcagggca tcgggcgctt cgaggggctc
    10441 gtggcgaagg aggtgctcgg cgcgacgcgg gactacatgg aggtcgcgta ccggggcggg
    10501 gacacgctct tcgtccccta cgagcagatg gagctcctgc acaagtacat cggcggtgag
    10561 ggcacacgcc tggacaagct cggcggctcg acctgggccg ccgttacgga caaggtccga
    10621 aaccgcgtca aggcgctcgc cggggagcta ctccaactct acgcccgccg cgccgccgcg
    10681 ccgggttacg ccttcccgga ggactccgag tgggagcggg agcttgagga gagcttcccg
    10741 taccaggaga ccccggatca ggaagcagcc atcgccgcga taaaggcgga catgcagagc
    10801 ccgcacccga cggaccggct ggtgtgcggc gacgtcggct tcggaaagac cgaggtcgcg
    10861 gtgcgggcgg cgttcaaggc
//
LOCUS       DASSMB010000008         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_888564, whole genome shotgun sequence.
ACCESSION   DASSMB010000008 DASSMB010000000
VERSION     DASSMB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888564"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFJ72_00665"
     CDS             complement(<1..432)
                     /locus_tag="VFJ72_00665"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET7478003.1"
                     /translation="METNFGASAPYTVGVEEEFQLVDPSSLALTPEIDAVLAARDGAG
                     LSADVVTSELSASCLEARTSPCGTVADLAKEVSGLRSRVRKLVEGCGARLAAAGTHPF
                     SDSMEQPITGDNRYRQVEERMGWTARMQAIYGLHVHVAVPDA"
     gene            600..1367
                     /locus_tag="VFJ72_00670"
     CDS             600..1367
                     /locus_tag="VFJ72_00670"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7478004.1"
                     /translation="MRREVSEKGVWKVGELAGRTGVSVRTLHHYDEIGLLSPSGRTAS
                     GHRLYSADDVLRLQQIRSLRALGFRLEEIRRCLEDSDLSAQRVIELHIRGIEEQIQRQ
                     NDLLGRLRAVAARLRSAEEVSAVELVNTAMEVIEMSERVERYYTKEQLKYLEQRRREF
                     GEERIRGFEAEWAELIEQVNAEMEAGTDPADGRVRRLAKRWMELIAEFTGGDPGIARS
                     AGNMWQQEENIHGMDTAHMRELGAYVSKSLAASDRAE"
     gene            1585..>2480
                     /locus_tag="VFJ72_00675"
     CDS             1585..>2480
                     /locus_tag="VFJ72_00675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET7478005.1"
                     /translation="MPELHWIGKRHVLDLADRVPTRSLRRVPEISMPPGSGNGILHGD
                     NLLALKALLPRYRDRVKLVFIDPPYNADAERWTYSDPIETPEMRGWRGEVGAEGEDPF
                     RHDKWLCMMYPRLTLLRDLLAPDGTIFISIGDNELPRLRLLMDEVFGARNFLGCAVWN
                     KGPAQDDDAGLLTSTHNYIVVYARDRAQASTSEDLTTWWTREDFGDSEEARRELEDLF
                     PEASALFSIPKPVRLIQRIVQAATTPHTRDIVLDCFAGSGTTAHAVLKQNERDGGNRR
                     FILIEMQNFTGGLTAGRVRRAIR"
BASE COUNT          432 a          805 c          839 g          404 t
ORIGIN      
        1 ggcgtccggg acggcgacgt ggacgtgcag gccatagatc gcctgcatcc gggccgtcca
       61 gcccatcctc tcctccacct gccggtaacg gttgtcgccc gtgatcggct gctccatcga
      121 gtcgctaaac gggtgcgtcc cagccgccgc gagccgcgcc ccgcaaccct ccacgagctt
      181 cctcaccctg ctccgcaggc cggacacctc cttcgccaga tccgcgaccg taccacaggg
      241 cgaagtacga gcttccaggc acgacgcgga gagctcggac gtaacaacgt cggcggagag
      301 cccggccccg tcgcgggcgg cgaggacggc gtctatctcc ggggtgagcg cgagcgagga
      361 cgggtccacg agctggaact cctcctcgac cccgacggtg tatggcgcgg aggcgccgaa
      421 gttggtctcc atccggctac tctagcagtt tggcagcgga gcttcggggc cttcatgttc
      481 cggttcgcgg ccctgcgggt ggtggatttt tctttttttt cgggatctcc cttgaacctc
      541 acgtaacgtg agggcgtatc ctctggtttg tagacgttgg cggccaggag agaaacgtgt
      601 tgcggcggga ggtgtctgag aagggcgtct ggaaggttgg agagctggcg ggccggacgg
      661 gggtctcggt acgcaccctc catcattacg atgagatcgg cctgctctcg ccgtccggac
      721 gcaccgcgtc agggcatcgc ctctactcgg cggacgatgt gctgcgcctg cagcagattc
      781 gctcgctaag ggctctgggt ttccggctgg aggagatccg gcgttgtctg gaggattcgg
      841 acctgtcggc gcagagggtg atcgaactgc acatccgcgg gatcgaggag cagatccagc
      901 gccagaacga tctcctggga cgcctgaggg cggtcgcggc ccgtctacgc tcggcggagg
      961 aggtctcggc cgtagagttg gtaaacactg ctatggaggt gatcgagatg tccgagaggg
     1021 tggagaggta ctacaccaag gagcaattga agtacctgga gcagaggcgt cgcgagttcg
     1081 gagaggagcg gatacgaggc ttcgaggccg agtgggccga actcatcgag caggtgaacg
     1141 cggagatgga ggcgggcacc gacccggcgg acgggagggt gcggcggctc gcgaagcgct
     1201 ggatggagtt gatcgccgag ttcaccggag gggaccccgg catcgcgcgc tccgccggca
     1261 atatgtggca gcaggaggag aacatccacg gcatggacac cgcccacatg cgcgagctgg
     1321 gcgcctacgt ctcgaagtcg ctcgccgcgt cggaccgcgc ggagtaggag cgccctccag
     1381 cccgtgaacc gcgcgccccg cggtcgggaa acaggcctga gaccgtgctt tggttgcgga
     1441 gcgctcgtgc ccgacgccga cggaccggcc caccgctatc tcggcgcctc accgggttgc
     1501 tgggccgccc tctaaccccg ccagccgtct cctcaccgag gctatactac gtcgcgtcga
     1561 gtagtagccc cggagaggat ccgcttgccc gagcttcact ggataggtaa gcgtcacgtg
     1621 ctcgacctcg cggacagggt tcccacgcgc tccctgcgcc gtgtcccgga gatatccatg
     1681 cctcccggat cggggaacgg gatcttgcac ggcgacaacc tgctggcgct gaaggccctc
     1741 ctcccccgct accgggaccg cgtcaagctc gtcttcatag acccgccata caacgccgac
     1801 gccgagcgct ggacctacag cgatcccatc gagacgccgg agatgagggg gtggcgcgga
     1861 gaggtcggcg ccgagggcga ggacccgttc cgccacgaca agtggctgtg catgatgtac
     1921 ccgcgcctca cgctcctgcg ggacctgctc gcgccggacg ggaccatatt catctccatc
     1981 ggcgacaacg aattgccccg gctgcggctg ctcatggacg aagtcttcgg cgcacggaac
     2041 ttcctgggct gcgcggtctg gaacaagggt cccgcgcagg acgatgatgc gggcttactc
     2101 accagcaccc acaactacat cgtcgtctac gccagggaca gagcgcaggc ttctacctcg
     2161 gaagacctga cgacgtggtg gacgagggag gacttcggcg atagcgagga agcccggcgc
     2221 gagctcgagg atctcttccc ggaggcgtcg gcgctcttct ccatcccgaa gccggtgcgt
     2281 ctgatccaac gcatagtaca agccgccacc accccccaca cccgcgacat cgtcctggac
     2341 tgcttcgctg gttcggggac caccgcccac gcggtcctga agcaaaacga gagagacggc
     2401 gggaaccggc gctttatcct catagagatg cagaacttca ccggaggcct gaccgccggg
     2461 cgcgtgcgcc gggccatccg
//
LOCUS       DASSMB010000009        41183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_382245, whole genome shotgun sequence.
ACCESSION   DASSMB010000009 DASSMB010000000
VERSION     DASSMB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 41183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41183
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382245"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..706
                     /locus_tag="VFJ72_00680"
     CDS             62..706
                     /locus_tag="VFJ72_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7478006.1"
                     /translation="MKTHRVLLVEDHPALRQALAWVLEEEPDLTVVGQAGTLAEAREV
                     SGGVDLAIIDPGLPDGDGVELIPELRERNPDCRVLVLTASLDHLDFARAVEAGAEGVL
                     NKTVSIQEILNAVRRLRAGETLLSAGEVIELMGFAALQQDRDEAARAILESLTARERE
                     VLAALAAGNDNSEIARRLGIAIETERYHMAHLLTKLGVKSRLQALLFALRHNFV"
     gene            990..1703
                     /locus_tag="VFJ72_00685"
     CDS             990..1703
                     /locus_tag="VFJ72_00685"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HET7478007.1"
                     /translation="MSGSVRPRISRRSQETELTLDGVSGGVSRKSVKVSRALLYSALL
                     VLVALAALGAYMATGVFTGKQDSSTYAMAQDIPTSFGVVAVEYVQKTPGLTAKQLGGM
                     THGIGSNIPPDKVQVQANVVLTNITNHTVDYSPEQFSMISGSGEKKIPLTSATLQGGT
                     LQPDASISGMVSFVAPRDGSNLAIDFNDNDGKDIVIDLGRTDTTPSNALDGYHHHGAD
                     SHQGRDEGAKQDGRSPPSN"
     gene            1769..7789
                     /locus_tag="VFJ72_00690"
     CDS             1769..7789
                     /locus_tag="VFJ72_00690"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HET7478008.1"
                     /translation="MKAVIPANFQQFVRSPWTLIQQSGMILYAAALLSLAAALIHLWV
                     TPEHFGEWWGYGSFMLAATVCQGLFAVVLLRWPGKLVLRAGIAGNLAIIGLYGITRFF
                     EIPLGPHAGHIEEIGATDLLCTASEIGLVALLAVLLRGLRSADAFASSRPSARPLALG
                     LFSFVLAYGGGLWLYLLHRAEGAVELNEPPFLIHWLRDSTLALPLVFLAVWMGIGLAR
                     RILDGREELSPVLSGAVTTATVAALASVFMASGSPVHEVLFNAHDGGHDLPVYLHVGR
                     DALFALAVNLPLAALVSLMLRGDPWSRPEASSRVRYLQFKGRFIAQGATAVLGLLLPA
                     MILAWGTFGAAPSATAAIDPGAPCPTAAPVKTFDVTAIDVDITLNRFGDHDPAGKMYV
                     LTGIDGRATNGVAKVRQEETAPLPGRVSIGLHGHDAIQPLVIRVNQGDCVKIDFHNQA
                     TGGDYGVHIDGLAYDTGSSGDQIGNNASSAVPNGASQSYMYYVPNDDALEGSHYIRPG
                     PGNRESISHGLFGSLMVEPPGSKYLALDSGNPVDSGWEASIVPGGATADTSDDAPSFR
                     EFALMHHEIGNEKFKIKNVDGGDLPTVDPHTDSYRPGSRALNYRSEPFMNRLDLDHEQ
                     KSLSYNSYTFGDPATPMPRGYLSDPTKIRLVHAGTEMFHVFHLHGGGDRWRFNPLADT
                     TFNYADTGLNKHPKTTHSPSSRLDSQAIGPGESYNLEIEGGAGGVQQSVGDFLFHCHI
                     AKHYNSGMWSFWRVYNTLQDNFKPLPDRVPLPKAVDSAGLIGKNFNGTTITKDNLKSW
                     VEQQVPTQGVPKNTQDASVWNWKWDDSNPAGSVALGEPEDQTPWPNHPGLLWPDLADG
                     SHPGGQPGDQFVGDRPKIMFDPVSGHPAFPLLRPHIAQRSPFAGNGHSGTPYLGASGD
                     QAPNPNAPGGVDPYANRDDGLCPSKTAGGAPTPLRNFDITSFVTPIQVTAKGKTDQTG
                     MIFALNKDKAAIQAGNKPAQPLAIRGNIGDCIQINFNTELTDAASFGNFAKGNMHIHH
                     VQFDIQGSDGASAGFVYDQSVRPYQAVDPQLTQAANAGDTVLHLSSVAKFQNGVWIEV
                     GEGTDNLDVRQIKSIDSAANTVTLTQPLAKSHATNEWAGTEFMRYRWYPDVELDNIFW
                     HDHVDGIHGWGKGLVGQLIIEPEGSTYHDPKTGTQVDSGTIVDIHSNNPLAPGVPGSF
                     REMALWTIDENPATDSTLNLRAEPWADRLANNGDPSLLFSSYTHGDPNTPLPKAYPGD
                     PFVIRTINVSGGVDTLHIDGQRLFVENRYADANGKTEASPLDTIHYGISEKYTAILEG
                     GAGGPNHIPGDYLYQNGIGRRFTQGAWGILRVLAGQSPDLQPLPGHPAPAGGGLPSPN
                     NGRPPAASGPGNPCPAGAPQRDFNVSAVDVPGAAPGSGHELAFVPTAAGAAAANEPLV
                     LHAAAGECINVTFKNESSNDRASFHVGKLERTIESSGINVGFNPEQTVGIGGTRQYRF
                     YADTEKIGSAVISDFGGKPVVPDPVTGAIPTNVDTGPQGLYGAIVIAPQGATFSDPTS
                     GAATDVGTQVDVHLPGAQPSGYRDFTLAFAEDDPRIGQDGMPYPTDVAGPALVNYTSA
                     PLLNDTNAAFSGNQPTPLLRAYRGDPVKVHAIGAPGSEQMHVFNLGGLSWPIDAYIDN
                     SNDMTNMGFGPLESIDAEINGGAGGQSHTTGDFYYGDMRRPFTQAGMWGLFRVSSDAS
                     CAPDAKSLKPLPGRDCTGAVDHDAPTLTADPGAGVFNASQQIKILSSEPAKIHFTTDG
                     SKPTQRSPLYKTPLTVDKSETVKAIAIDQAGNRSAVASWDFTIDTVAPTVSANPKPGT
                     YDQAQQVKLQASESGSRIFYTTDGSTPTPQSTELQPGTAIPVSVSGSIKAIAVDPAGN
                     ASDPATFAYTIRQASKVTLNVATTPLRLGKVRTIAGVVSPSHAGSVRLTIKRNGKTVL
                     AKSLPLKSSRFSMPYTPRAVGSYSVIVNFAGDADHKPSTANKSFNVVR"
     gene            complement(7863..8582)
                     /locus_tag="VFJ72_00695"
     CDS             complement(7863..8582)
                     /locus_tag="VFJ72_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478009.1"
                     /translation="MAQEFRLPGSTYEELVNIIVAYGTRDEASNPGDVGKLNSVHQSS
                     VSRNNAFLTGIGVLRGEREKLVTRRGRSLALALARKDREGIRDNWRAVVSTSEFLQNV
                     VSAVKLREGMLYPTVQAYIAHAAGQPRNKPVMSGAGALIEILKAANLLKEEAGELVAT
                     FDEDLKDELPEPEEEATPEERPAKETVSAVVAGEDIEATGEEGGGAKVQVHLHISCTA
                     DEIEDLGPKLKALIQDLSSPR"
     gene            8883..9389
                     /locus_tag="VFJ72_00700"
     CDS             8883..9389
                     /locus_tag="VFJ72_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478010.1"
                     /translation="MEGIFYTTSEVRDMTLKQKPDAPPITYPSPAEDEPRPAGVGSRW
                     LGFALVVIGVGLISLAATDAFAGAATGGGADTLRGSEPGERLNGYAGDDYINGYAGDD
                     ALSGGDGADEVYGGPGRDLLFGGAGDDFIEAKDGVADYVDCGPGDDVASVDGADRASP
                     SCETVYPG"
     gene            9414..10904
                     /locus_tag="VFJ72_00705"
     CDS             9414..10904
                     /locus_tag="VFJ72_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7478011.1"
                     /translation="MRFGAHTLTRRDFLMLAGAGAMGASGLSLVGAREYEKVTGRRIS
                     LGEQMNVIMIVIDSLRKDHVGAYGNTWIQTPNLDAFAKESLRFTRAQPEATPTIPARR
                     TIHTGLRTWPFRDWDPHKGDSVMLYGWQPIPEQQWTTAEILQAQGFETMLVTDAYHEA
                     KAAMNFQRGFNVFDIIRGQENDHFHPMWMCPQDKIENTLLSGDRRNVEGKMRQYFANT
                     TGRSGEQDYFSPQVFSRAARWLEGASKHGRPFFMSVDSFDPHEPWDPPEKYAKLYSDG
                     YAGPEPYTSVYGSSDYLEEAELDRMKALYAAEISMVDHWLGNFLDKVESLGLMENTMI
                     LLLSDHGHALGEHGAVGKPPYALWPELTDVAFMIRHPEGKRAGDESDYYASTHDAAPT
                     VLGALGVEPPSPMDGQDLMVFFEGREPEARPYFVGGLHDHLWVRDGDYAMISRNTGSG
                     AKLYDLKIDPTQQKDIAWRKPDVVKRMFDDYVIKAAGGEPPPNYDY"
     gene            complement(10898..12997)
                     /locus_tag="VFJ72_00710"
     CDS             complement(10898..12997)
                     /locus_tag="VFJ72_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478012.1"
                     /translation="MLLDLTPGLFAAVAAAVVPGYFWSRLFATTADRAERLAFSTALS
                     ITLVPATALALIGFSGTGVTLAIAIVSVLAVFVSGLAAYRIFSSPKGLDEPIIPVTTP
                     PGPLALALLALAFGLVAAAYSGILQGRQVMLAVALLLLFAGLVYTVEVRRGDPVASAS
                     RELEPRWPAMRRLLLPMVLLLVLFRGYSGPVLHDWPYLRGGDQYFHAMMANMMLTRGK
                     IEPYAVYPPGLHTLDAVISRLSGLEPVALFPVYTPALILLPPLALYALARRMWGWEYG
                     VAAAALSGLVCNSTFIYLSYQAMYPTLVSAEFLLVMAVAALVGLYARPSARSVLLLAL
                     LGSSVALYHPVGSLYAAALFGLVGVLILPYLLLRERRTGLALLGSFALLGLLAAAYAW
                     DTYNLPQTVADLLRGSGTTAGGQAVSMAVGTQAPLALEHFLKTITQPVLWLGLLGALL
                     MLGGREGPLTSAMSRSTLLLWCLVLFVGSRTSVSGFPERFERDLAIPLSLLAAFALVA
                     ILRSLRGSGMLARAAAVAVAALVAGVIGVQAVWNLHAAAKPSPRVVLTPQIVAAGEWL
                     GAHNTGGNIMVAPRGNQVTGRAMLALSGYFALEAFTEGRIHYDRDLPPYGAGPLLDVL
                     YVIKHPDDERTRQLLEKHDVRYVVFYKRFGEGTTWDRTPVQDWRGHVAQKDLYRVAFE
                     NEGVIVLEPRPAELRDQ"
     gene            13365..14570
                     /locus_tag="VFJ72_00715"
     CDS             13365..14570
                     /locus_tag="VFJ72_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-sulfoquinovose synthase"
                     /protein_id="HET7478013.1"
                     /translation="MKVLVLGGDGYCGWPTSLHLSDAGHDVVIVDNFVRRTIDHELGA
                     GSLTPIADPKERVKAWKEVTGQTIGLEYGDLLDWEFVSELFERHKPEAVVHFAEQRAA
                     PYSMIDRRHAVYTQHNNVVGNLNVLFAIQEYVPECHLVKLGTMGEYGYPNIDIEEGYI
                     EIEHNGRKDTMLYPKLPGSWYHLSKVHDSHNIHFACRIWGMRATDLNQGIVYGTVTPQ
                     TERDPRLMNRFDYEEVFGTALNRFCIQAAIGHPMTVYGKGGQTRGFLDIRDTVRCIEL
                     AVDNPADRGEFRVFNQFTEQFSVLQLAEMVADASAKLGIEARLENVPNPRVEKEEHYY
                     NAKHTGLIELGLEPHYLSDSLLDSMLNIAIEHKGRVDMDAIMPSVDWRTGKRTARERA
                     AAPAESPAL"
     gene            complement(14633..16750)
                     /locus_tag="VFJ72_00720"
     CDS             complement(14633..16750)
                     /locus_tag="VFJ72_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478014.1"
                     /translation="MIWDLIRALAAATAAGVIPGYLWASCLCPTADRIERFVYGIGLS
                     LMLVPAMGLLQAKLFGTGLSLPIAISSVLLVFGGGLAFCLKFGPVKKPHGPLAPPSTP
                     LGLPTLIPLIAALGVAFAAESHAVLAAWTGPLALLLVLAAGLARLLDTGIPTVPASTT
                     DDTVHEPERKPETVVRYLLLGVVLSLTLARGYLGPLRYDWPYIRGVDDYEHVVMTNMT
                     ISTGSTDSFMLYPPGFHYLMATISQLSGMDAMKIFPVLAPMLLTLVALGCYALARRIW
                     GWEAGIVAAFFAGVVSYGSYMHFVEARYPNLLTVHFLFTLAVGALLILLAVPTRRSGV
                     LLALLGSSAVLYHQVATLYEIVLFGLAALCFLPYLLLRDRRRGLAMLYSFAALGVLAV
                     VFAWDTYDLPQAISGLFGGPGGGRGDDALKMAIGTQPPLSLTHLLAMISPPVLWFGAL
                     GAVLLALDRGRTDPPYAFGRAMLLIWGLLLFIGSRTSASGFPERFERDLAIPLSLLAA
                     FALVQLLRSLQPRMPVTVFATSLATVLAAALIGVQTVRNLDVAGGPASQIPPQLITSA
                     TQRMLTPKIEAAGNWLRDHNTGGNILVSPYLDLVPSRAILALGGYTGMQSYDSGRIEL
                     ARDLPPFGAKPLKDALFMLGHPDDERTLQLIEKYDVRYVVLYKRVPPPRGMDFQTFEK
                     SSNYKKVFENGRVVIFAPGETKR"
     gene            17013..18074
                     /locus_tag="VFJ72_00725"
     CDS             17013..18074
                     /locus_tag="VFJ72_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 family protein"
                     /protein_id="HET7478015.1"
                     /translation="MTAEHVRIVGIVVALLFAGYGIIRYRGGEQSRFDLLTALLIAAG
                     VATVSTIPQIGEVFVRLLGLQNRAFALLSVATLLLFGLYLHLLGRVREASRRNGEIVT
                     GLAVREYEENYLSPEEQLPERLAERREILIVVPAYDEEGGIREVLRRIPDEVLGYQVK
                     TVVVDDGSTDATAAITREEGVPIVTHVVNRGQGDALRTGFAIAIKEAADIVINLDADG
                     QYKPEEIDRLVKPIVEDEADFVLGSRFMGFYEEAGSVRHVGVVFFSRMISLLTGVKVS
                     DCTNGFRAIRVSELPKLNLREDRFNANEIILEALKNKLRFTQVPISMMSRAAGETKKP
                     PKLAYPLGVFRVIISTWLR"
     gene            18118..19395
                     /locus_tag="VFJ72_00730"
     CDS             18118..19395
                     /locus_tag="VFJ72_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET7478016.1"
                     /translation="MKNFLSAAKGRQGAGAGSLYIAFSFAVSGALTFGFQSLAAHALG
                     KAGYAPLALLWSSTFLVVQVLWIGATQTLGRYIAEREAKGQDWRPVIRSVKRWQIGLS
                     GAFVLVALPISPWLTGLFGDPWLTVVFVVSMVAYAPEYFRRGIFNGHHQSSRLGAQIL
                     AESAGRLLIAAALLAAGMGVLGPAIAILLAPIIGVLAVRPAPAEPPEHEGEPFSAMNA
                     LKFAGPVLTCVAFAQVLMNGGPILVSVLGGTPAQVGLFGAAMILTRIPQYIISPAIGA
                     LLPHASRILSTGGYGPFGRFVGRAVGVVGLVGVLMVGATWLLGEWGIKLFAGSEFDAS
                     REMLVTLAAVAAFYLLCDMLNQALFALGHARLASLGWLVGLVVSALCLAILDTDVLSR
                     VSYSLAIGTLAAAMAQAAFYLMARRSATVSRSG"
     gene            complement(19403..20377)
                     /locus_tag="VFJ72_00735"
     CDS             complement(19403..20377)
                     /locus_tag="VFJ72_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7478017.1"
                     /translation="MKWLITGGCGFIGTAVIQSLLEEGGHDIRVLDKLAVGTREDLAS
                     VCEFSEGEGNGPIVSGVELVVGDIMDEELALRVVEGADVILHLAANTGVEPSVKDPRY
                     DCVTNIVGTLNYLEAARHGGAGRFVSASSGAALGAVDPPYHEELPAHPVSPYGASKVA
                     GEGYCSAYFRTFGVDTVALRFGNVYGPLSGHKNSAVAKFIRRARNGEALEIYGDGTQT
                     RDYIYIDDLVEAIKLSATAEGVGGEVFQIATSTETTVRELIDELLPVLASAGINDVEV
                     RQTTPRQGDVQRNYSDTSKAEKMLGWKAEVALPDGLRRTVGWFMERPA"
     gene            20534..21145
                     /locus_tag="VFJ72_00740"
     CDS             20534..21145
                     /locus_tag="VFJ72_00740"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7478018.1"
                     /translation="MRKGERTRRRILERSAPVFNTKGYFGASMSDLIRASGLEKGGIY
                     NHFGSKEELALESFDYAAGIMRARFDAALADREGAIERLFAVVDVLGALVEDPPVAGG
                     CPVLNTAVESDDAHPALKARAEEAMIGWLRLIGRIVKDGARDGELRTDTDPREVASVV
                     VATLEGALMISKLCDDPSHMQRAVGHLKGYIETLATRDGRSEV"
     gene            21147..21710
                     /locus_tag="VFJ72_00745"
     CDS             21147..21710
                     /locus_tag="VFJ72_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478019.1"
                     /translation="MKVIEEDWTVARAVFAPGAESPGVPRYGGNIRVRAVERGDLDGL
                     WRMLSRSSSDTIYQRFHAPYPRVPERVMELLVDVDPRDGKALVAVAGEEIVGHAMYAR
                     TRDEEAEIAVVVEDGWQSRGLGRLLIFEIASVARSRGVEVLYCSALVENRRALDLSAL
                     MFGEVESVFSGGLRWIRVPLRSLKEKV"
     gene            21707..22240
                     /locus_tag="VFJ72_00750"
     CDS             21707..22240
                     /locus_tag="VFJ72_00750"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7478020.1"
                     /translation="MTETMDRTLTVSWEDPAPGAEAARTMSGLEYLRAVAKGDLPAPP
                     LAHLVGFGLHEVEEGRAVFECEPGEQHYNPIGSVHGGLACTLFDSAMGCAVHTALPAG
                     TGYTTVEIKVNLLRPITVDTGKLLCEGKTIHVGGRIATAEARLFDEAGKLYGHATTTC
                     MVFRDEANGSSVGEGTS"
     gene            22237..23226
                     /locus_tag="VFJ72_00755"
     CDS             22237..23226
                     /locus_tag="VFJ72_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7478021.1"
                     /translation="MRVARVEEFGGPEVLRVAEEPRPEPGESEVLIELRSAGVNRADV
                     LTRSGRYHAAGQPPIVPGFEGSGVVRKTGASVTGASVGERVIAFGGRPGFYAEYVAVP
                     EEHVVAMPDGLDEDSAAALPVAPLTAFYCLRRLARVEPGETVLVWAAASGVGDVAVQL
                     AKAAGAEVIATAGTDEKVAWTLENGADHGINHSIESVVDRVVELSGGSGVEVVLDTVG
                     GARFGESLKVAGHGGRVVSLANVALEGSALDTRDFYPKNVTIYGFQITNLMQRLGYDP
                     REDLAELANLVVDGSLKVHVDRAFPLEEAADAHRYLEERRNRGKIMLHPGEGG"
     gene            23223..24122
                     /locus_tag="VFJ72_00760"
     CDS             23223..24122
                     /locus_tag="VFJ72_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7478022.1"
                     /translation="MSDPLGEFPKPSPPPAPTTSDLSGFEERVEHRYAENEGVRIHYA
                     VLGEGPLVVMMHGFPDFWYTWRHQMNALSQNYRVAALDLRGYNLSDRPDSIESYGMKS
                     LLGDVAAVIRAEGYEKAVIVGHDWGGAISWQFAERLSGMTEKLVILNLPHLTGLTREL
                     ANNPDQQRASSYTRFFQQEGAHESLSAGKLARWVSDPDAREKYVEAFERSSFEAMLYY
                     YKRHYPAEPYTELGPPQAKVPVPVLVIHGLQDPYLLPDALSGTWDWVERDLTLVTVPE
                     AGHFVQQDAADFVTRTIESWLAR"
     gene            24119..25417
                     /locus_tag="VFJ72_00765"
     CDS             24119..25417
                     /locus_tag="VFJ72_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478023.1"
                     /translation="MKGESRGRLYYGWIVVAVVFLALLVSAGVRAAPTVLINPLENEL
                     GWGRATISFAVSIGLLLYGLSGPVAGWLMDRFGPKLLTLAGLAVIGGSTLAGATVTEL
                     WQLNLFWGVLSGVGTGVVAPVLGATVANRWFFERRGLVLGIFGAAASAGQLVSVPALM
                     WLVVAVGWRQGVVFLALAALTVIVPILLFMRDDPRKMGLRPYGDTESEEPAAQSSPAP
                     EPGTPLADVDPQRSGVLRRAVRAPEFWLLSGSFFICGASSNGIIGVHFIPHSIDHGIP
                     EVTAAGVLALMGAMNFVGTIASGFLTDRYDPRKLLAVYYSLRGLSLLLLPFVTHFEGL
                     AIFAVFFGLDYIATVPPTVALVADRFGRMNVGTVFGWVFFSHQLGAALASYLGGVARD
                     SLGDYTAAFIIAGILAGMAALMALLVRRQRPPAAGEPVRA"
     gene            complement(25418..26365)
                     /locus_tag="VFJ72_00770"
     CDS             complement(25418..26365)
                     /locus_tag="VFJ72_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7478024.1"
                     /translation="MKSGRLLATGGAVGGLALYNQRLARSGGEIGNRIGGETRYFRWK
                     GEDLAYAVAGEGPPLLMVHGVYAGASSFEFLKNFLELSESFRVYALDLLGCGLSARPR
                     RRYEPEDVSSQIEDFAREEIGNPAHLIASSLTAALAMPALVRSPRLFKKAVLICPTGY
                     GSLDRPSGVLGDFIQGLFSAPVIGDSLYHAIVSRPGLRYFLGGMSYHDRSFVTEELVE
                     SYYRISHQPGAKHFPAAFVAGKLNLGVADYWPRVPHRTMICWGQEAKTTPVSELQTFL
                     THNPRTAPRVFRDAALLPHDERADTFNQQVTEFLLAKGA"
     gene            26461..26931
                     /locus_tag="VFJ72_00775"
     CDS             26461..26931
                     /locus_tag="VFJ72_00775"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7478025.1"
                     /translation="MDREQLIDEVLMLLPALMRLVGRPGPVEMGEIARRGIPVDAHIS
                     PGHVQILISLSRGPRSVGQLAEDVGVSPPAATQLIDKLVEHGMVERRHDHADRRVVLV
                     DYVPGMHEVARRIMEDRRKPIERTINSLTDEEARAFVKGLKLMTEGLGTGPVES"
     gene            27138..30656
                     /locus_tag="VFJ72_00780"
     CDS             27138..30656
                     /locus_tag="VFJ72_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7478026.1"
                     /translation="METIVRWCLKNKSVVILAALLLIGAGAYATTRLNQELLPDISFP
                     VIVVSTPVSGAGPDVVDEQVTQPVESAIKGITGIESVQSTSSQGFSVVVVQFNLDTDT
                     EEAEADLQSALDGIQLPSQAGTPEVKSQSASQFPILNLSLAAKDRDLADLTKYAEDDV
                     VPAIEEVDGVASVDLIGGAEKRIQVDLDPKKLKEKGLSTEAVVGAISGAEVDSPVGSV
                     PVGGLDTPIRATSKLGGVKALEDLPVGVEGGSASAAPTGTASSASTNVPAGIPAGIPA
                     GATGAAPSGSASPSGAPAAGTTAAPTATAAGDQAPKPVLLGDVAEVREVGSDLSGISR
                     TNGEPSLGLNVIKETDANTVEVAADVEKVLDDARDKIGDGQVVVVSNSATDVEESVNG
                     LVEEGLVGAALAILVIFAFLRSLRATLVTAVSLPTSVLAALLFSWADNLTLNIITLAG
                     LTIAVGRVVDDAIVVLENSYRYVQRGYEPEEAALKGTTEVASAITSSTLTTTAVFLPL
                     ALVGGIISKFFVPLSLTVALALLASLIVAVTIIPVLVSIFVKRRAGDYTPDASADESD
                     DEPLRRVDMRSRRGGLARFLVGVVVFLIASAAAVVIASLAGVLGRLPGLPQSYAEAVD
                     GFVSGVGFGSPVFLAVVGVAGALLLAGLVLLVVRAARRPSGGESDGVLVRLYTPMLRW
                     SLRHRLAVLVLAFLAFAGGLGLVQLLPVSFFPPSEERLLIADVELPAGTSLQKTSDRL
                     RPFEDFLGKDRGVKSYQASIGGEDTLDPESPVRPGNKAQAFINVKASANVGRTLDRID
                     AEGDDLYGENFKIQVISNGPPQGGLEAILTGGSKKDRADAADMVSKEFNKLDNVNNVE
                     SDLSGGNPEVQVTVDPEKAAKVGLSPGGVSSSLGALLGGSTVTTLGDTPVVVGAPAKT
                     VDSLDEVRKLSVGSGSTVGDVAQVDQVESPAAVSRTDGDRAVTVTGEINSNDTQTIST
                     KAQAAVAKLDLPGNVKAQVGGETDDIDESFRNLFLSIIVALVLVFLILVVFFGSLLIP
                     MVILLAVPLTTVGAFGALYLTNTALSVPSLLGILLLIGIVVSNAILLVDFAVKALDHY
                     ETPDEAILAAGQARLRPILMTAFATVFALMPLALGLSGGGNGLISSSLAITVVGGLAT
                     STFLTLLVVPVGFSFLKSGLRRKKKV"
     gene            30660..30857
                     /locus_tag="VFJ72_00785"
     CDS             30660..30857
                     /locus_tag="VFJ72_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478027.1"
                     /translation="MEQKIEAGDRAPGFSLLAAGSGRRLSVRKSEGRSLGLIFYPQGA
                     APAARRSTGPSAPVIRPRTTF"
     gene            30884..31144
                     /locus_tag="VFJ72_00790"
     CDS             30884..31144
                     /locus_tag="VFJ72_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478028.1"
                     /translation="MPPVYRMAVGMVLNTSYEQAAEEIPQDMNPAEYVINGPDYGGLM
                     SRRYGVRNTGRAAAIVDGDSNISGSYQGERPVENVFEMLDAR"
     gene            31276..31482
                     /locus_tag="VFJ72_00795"
     CDS             31276..31482
                     /locus_tag="VFJ72_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478029.1"
                     /translation="MRESLRQRLEDGPARIAREEVEEIGADIGMEPVDAVRLFDRLKG
                     VSWRGDYVESDGGRWAAVWVTEVN"
     gene            31552..32232
                     /locus_tag="VFJ72_00800"
     CDS             31552..32232
                     /locus_tag="VFJ72_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HET7478030.1"
                     /translation="MDVREERPFRDRADAGRRLAGRLENYRDEEPLVLALPRGGVPVG
                     FEVARALGAPLDVFVSRKLGAPGQPELGIGAVAQGGTRVLNERLVRRLGIPDEYLEYI
                     TRREQAEVERRLRALRGDRPEPEVRGRTAILVDDGLATGVTAMAAIEALKTRNPRRIV
                     LAVPVCAAPTAEMMRPEVDDLVSLRIPEDLYAIGFWYEDFEQVPDETVIELLKRARRE
                     GERDDREG"
     gene            32201..33250
                     /locus_tag="VFJ72_00805"
     CDS             32201..33250
                     /locus_tag="VFJ72_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET7478031.1"
                     /translation="MKGRETTGRDNLHVYTDAAMRDHVNGLSHVEVPERFPAAIAGVE
                     AAESAGVAVEWRASDPAPEEALLAVHERSYLEKLERVCGTGGGFLGTDTAAGPGSWEA
                     ALLGAGCAVGAVESALSGETAFAAVRPPGHHAGRATAMGFCLLNNAAIAAEHVRSLGK
                     DRIAILDWDVHHGNGTQDIFYEDGEILYLSVHRSPFYPGTGAAKEVGAGEGRGMTVNV
                     PLPRGTGEATYAVAFAGVLLPILRAFRPELLIVSAGYDAHADDPLGGMRLQAESFGRF
                     AATLDALVREIGAAPLVMLLEGGYDLAALTGSIASTLRGIGQEPPDWNMEGDPDDPEV
                     ENSREALEPYWHSLR"
     gene            complement(33318..34187)
                     /locus_tag="VFJ72_00810"
     CDS             complement(33318..34187)
                     /locus_tag="VFJ72_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7478032.1"
                     /translation="METMVTRRRERDADTPDLIPGYFARIDKGNLLTHAEEIELSRNA
                     KAGDTRARQKLIEKNLRLVVSVAKKYRGMGLPFEDLIQEGNIGLMRAVEKYDPERGWR
                     FSTYATWWIRQAVQRAVADKGRTIRVPVHMGEKIRKMARAYTELSGELERDPSDEEVA
                     ERIGWTADEVRDVKSAMPDATSLNQPLSSDGDASELGELVEDERASDTAGAVVREMET
                     EGLGAAIEQLPERQRHVLIRRYGLDAQEPATLAQLSDELGVSRERIRQLQREAEQMIR
                     AGEYGRFLDIVAA"
     gene            complement(34406..36085)
                     /locus_tag="VFJ72_00815"
     CDS             complement(34406..36085)
                     /locus_tag="VFJ72_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HET7478033.1"
                     /translation="MKIDFDRYLRYEELTDTLHALAGEHPDLCTITSVGKSYEGRDIW
                     LAELTNEATGPASEKPAFWVDGNTHAGEVTGSMSALYLIHTLLEGHGSDEKITRLLDE
                     QTFYILPRLSPDGAERYLTTPYTLRATLRPWPEPEEEPGLHAEDVDGDGHILQMRVED
                     PHGEWRVSDKDPRLMLPRAPDDADPRATYYRIYREGYFKDYDGFERKIARPLHGLDMN
                     RQYPYHWKGDDDQRGAGPYPLSEPESRAHVDALLARKNVFSAHTYHTFSGVILRPYSN
                     RDDLEMPDHDLAVYKALGERGTALTGYPNISVYHDFRYEPTKFITGTFDDWAYDSYGV
                     FAFTVEFWSAAKAAGVEVKDFIEFIRRPPEDAQIKMLAWNDRDLDGEGFVDWRPFDHP
                     QLGRVEIGGWKTKFTAQNPPPKFLEAECAALTRFALSNAEAAPRLEATLQAEEVSPGL
                     RRLELRVQNTGYLPTNVTRIAADKKLVQTVKAEIELPKGATLVSGDLELDLGHLTGRS
                     SLEGNSWKDSSFFVGLPSDYARRIVWVVRGDGPVKVEVRSEKAGTLRLSTE"
     gene            36252..37646
                     /locus_tag="VFJ72_00820"
     CDS             36252..37646
                     /locus_tag="VFJ72_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7478034.1"
                     /translation="MRHERYTVEGGEVLDASVRVLGGRYRIQGTLGTGGMAVVYKAED
                     AILGRKVALKTLHDRYAEEPAFRRRFKQEARAMASLDHENIVKVYDISQDGDNPFIVA
                     ECVSGKDVGDLLAQNEGGPLNEQFTRRVAEQLLRALSYAHRRGIIHRDIKPSNLLITH
                     EGTVKVADFGIARIVEDEDAGEPGEIVGSARYMSPEQLRGEEAIPASDIYSVGILLYH
                     CLTGEPPFSGDVKSLARQHMKSDPTPPRRLNKKISRHMEAVILKALAKDPKDRYTSAD
                     VMLDDLEAEGPSRKAIGTREAPKPAAVSKAPKKGKSRRRGLLLIPALVLLILAAGGGA
                     FAAGLVDVPSKQSIQQAFGQAQEVVSDPIEAPKASSADKASQPVKDDRKDEVSAEMVP
                     VPDVRVYYDYSAREILVNRGFKVRIVYSPQEGYAERGVAWGTKPAIGEMAPKGSTVTV
                     YATPKNLYQPQIRS"
     gene            complement(37669..38109)
                     /locus_tag="VFJ72_00825"
     CDS             complement(37669..38109)
                     /locus_tag="VFJ72_00825"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET7478035.1"
                     /translation="MQQDPRNYGRAEEPTDGALSPQDERVWSALSHASILVWPATGLL
                     PIAPLIIWLVYRDRSPQVGFHALQSLWYQLAWLAIATVGGILGTLFSIVTLGLGLFLV
                     IPLAAVLGLVPFVHSLYAAYRVYTGADFRYPYIADRMEGTGRTF"
     gene            complement(38130..38660)
                     /locus_tag="VFJ72_00830"
     CDS             complement(38130..38660)
                     /locus_tag="VFJ72_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478036.1"
                     /translation="MRSSTQAPELIPWNMLQDRLDGSFAARARGLLRASYTLTDRSGT
                     PFAELRARGGRVTLTAGSLEIEIEGDSRGGYRMLSGGTELARAASPGGSATVLRVECA
                     GQAYEARLRLLSNVTVVHTLDGSEEVSEVARLSGGLAGRRYKAVFDAEVQGALPVAIL
                     VLYHSVTLRRRIYLAG"
     gene            38747..39112
                     /locus_tag="VFJ72_00835"
     CDS             38747..39112
                     /locus_tag="VFJ72_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478037.1"
                     /translation="MELKNKEEIRTEILQSWLRNAWVYPQQGAGEQTFLRLTPGGRLK
                     MRRKIGELETSLGVSGGELAKQEEAGTLPVERDRMELAMMVQAYTSERKFIQSQGANL
                     GTPAVELEGEEPESGPEET"
     gene            complement(39109..39831)
                     /locus_tag="VFJ72_00840"
     CDS             complement(39109..39831)
                     /locus_tag="VFJ72_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7478038.1"
                     /translation="MPEWLPVEFDPSNFSLENIREKYLDFGLAFQNLDVIVQAVLVTL
                     VLALLAEVFGIVFGLVLALLKISRSRIISFPAQLYIDIFRGTPLLVQIIFIYYATPLI
                     GIRFQSLFFAGLTALTFNSAAYVAEIFRSGIQSIDKGQMEAGRASGLSYAQTMRHIIV
                     PQAFRRTIPPLTNEFVMLIKDTSIVSVIGLEELLRAARVLQSATANATPLVVAALIYL
                     AICLPLIYLTNALERRLNRKTA"
     gene            complement(39876..40700)
                     /locus_tag="VFJ72_00845"
     CDS             complement(39876..40700)
                     /locus_tag="VFJ72_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7478039.1"
                     /translation="MKRLNGVMLVALVFALSMVVAACGGGGGGETTGAGGTTSGLEGA
                     ASGQTVKVASDIAYRPFEFSEGGEPKGFDIDLMNEIGKRAGFTPEYQNVTFDGIIGGL
                     GSNLYDAAISAMTITPEREQQVDFSDPYFNADQSLLVASDSGIKSVDDLADSTVGVQI
                     GTTGASKAEEFKSQGKISEVRTFDTVEDAFAALENGQVDAVINDLPVSQDKANKSGGA
                     LKIVQTIPTGEQYGIAFPKDSELVAPTNKALKEIKDDGTYAKIYKKWIGTEPKEIP"
     gene            40900..>41183
                     /locus_tag="VFJ72_00850"
     CDS             40900..>41183
                     /locus_tag="VFJ72_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478040.1"
                     /translation="MREPDSAFVSAVTRAVEDRRDDAVRLLRALVNIPSVTGGEGAVA
                     EAVAHAFRERGLDVDVWEATREEMEPYLEHVGEQETYAGRPNVVGRRAGSG"
BASE COUNT         7321 a        13245 c        13620 g         6997 t
ORIGIN      
        1 gacgtgatat tatcgataca attaatccaa atctaagttc atcaaaggtt gttcgtctgc
       61 gatgaagacg caccgtgtcc tgctcgtcga agatcatccc gcgcttcgtc aggcgctggc
      121 gtgggtgctg gaggaggaac cggacctcac ggtggtgggg caggcgggga cgttggcgga
      181 ggctcgcgag gtgagtggtg gggtagacct ggcgataata gaccccggtt tgcctgatgg
      241 agacggtgta gaactcatcc cggagttgcg cgaacgtaac ccggattgca gggtactggt
      301 cttgacggcg agcctcgacc atctggattt cgcccgggcg gtcgaagccg gcgcggaggg
      361 tgtcctgaac aagacggtct cgatccagga gatcttgaac gccgtgcgcc gtctgcgcgc
      421 gggagagacg ctgctgtcgg cgggagaggt catcgagctg atgggtttcg ccgcgcttca
      481 gcaagatcgg gatgaagccg cgcgcgcgat tctggagagc ctgactgctc gtgagagaga
      541 agtgctggcg gcgctcgcgg cgggtaacga caacagcgag atcgcgcgcc gcctggggat
      601 agctatcgaa accgaacgct atcatatggc gcacctcctc accaagctcg gggtcaagtc
      661 gcgtttgcag gcgctgcttt tcgcgctgcg ccacaacttc gtgtagatcc ttcctgccca
      721 tagtcttctt ccgccaacga agcagggtgg tgcagccggg gatggacttt gggtgcgccc
      781 caaactgtcc cagacggtag attccggctg gttttctgat gccctgatgc cgctcggagt
      841 gcgccgcatc ttctgtgatt agtccacttg ggctagagaa ttcttagctc atccgggcta
      901 gctaaaaatc attaatccaa acgatggcgg atgtgtctcc ccgcttcatt attcaggttg
      961 tcagatatga agctcgacgg aggcgcagga tgtcaggcag cgtaaggccc aggatatcgc
     1021 ggagatcaca ggagacggag ctcacgctcg atggagtctc cggcggggtc tcgcgtaagt
     1081 ctgtcaaggt ctcgcgcgcg ctgctgtact cggctcttct ggtactggtg gcgctggccg
     1141 ctcttggggc ttacatggcc accggcgttt ttaccggtaa gcaagactcc agcacgtacg
     1201 ctatggccca ggacatcccg accagcttcg gcgtcgtggc cgtcgagtac gtccagaaga
     1261 ctcccggcct gacggcgaag cagctcggcg gcatgacgca cggcatcggg agcaacatcc
     1321 cgccggacaa ggtgcaggta caggcgaacg tagtcctgac caacataacg aaccacacgg
     1381 tagattactc tcccgaacag ttcagcatga tctctggtag cggcgaaaag aagatcccgc
     1441 taaccagcgc cacgctccag ggcggaactc tgcaacccga tgcgagcatc tccgggatgg
     1501 tcagtttcgt cgcaccgcgc gacggctcga acctggctat cgacttcaac gacaacgacg
     1561 gcaaggacat cgtcatagac ctcggccgca cggataccac tcccagtaac gccctcgacg
     1621 gctaccatca tcacggagcc gacagccacc agggccgcga cgagggagcg aagcaggacg
     1681 gcaggtctcc gccctccaac tagctccatt accggagcat ctccgctctt ctctcggcta
     1741 ccaatcgcaa caccacggag gcaacaaaat gaaggctgtt atccccgcaa acttccaaca
     1801 gttcgtccgc tcgccctgga cgctaattca gcagtccggc atgatcttgt acgcggctgc
     1861 gctcctctcc ctggccgcgg ccctgatcca cctgtgggtc accccggagc actttgggga
     1921 atggtggggc tacggctcct tcatgctggc ggcgaccgtc tgccagggtc tcttcgccgt
     1981 ggtccttctg cgctggcccg gcaagctggt cctgcgcgcc gggatcgcgg gcaacctggc
     2041 gatcatcggt ctgtacggga ttacgcggtt cttcgagata ccgctcgggc cgcacgcggg
     2101 acacatagaa gagatcgggg cgaccgacct cctctgcacg gcctccgaga tcgggctcgt
     2161 cgcccttctg gcggtcctgc tgcgcggtct ccggtccgct gatgcgttcg cgtcctcgcg
     2221 gccttccgcg cgtccgctgg cgctggggct gttctcattc gtcctggcct acggcggcgg
     2281 cctctggctc tacctcctgc accgggccga gggcgcggtc gaactcaacg agccgccgtt
     2341 cctgatccac tggctccggg attcgaccct ggcgcttccg ctggtcttcc tggcggtttg
     2401 gatggggatc gggctcgccc gccggatact cgacgggcgc gaggaactct cgcccgtcct
     2461 ctcgggagcg gtcacgacgg cgaccgtcgc ggctctcgcg agcgtcttta tggcgtccgg
     2521 gagcccggtg cacgaggtcc tcttcaacgc tcatgatggc gggcatgacc tgcccgtcta
     2581 tctgcacgtc gggcgcgacg ccctgttcgc tctggcggtc aaccttccgc tggcggctct
     2641 cgtatctctc atgttacgcg gagacccgtg gtccaggccg gaggcttcca gccgggtgag
     2701 gtaccttcag ttcaagggac gtttcatagc tcagggcgca accgcggttc tgggactttt
     2761 gcttccggcc atgatcctgg cctggggcac cttcggcgcc gcgccgtccg ctaccgcggc
     2821 gattgacccc ggcgcgccgt gtcccactgc cgcgccggtc aagaccttcg acgttaccgc
     2881 tatagacgtg gatatcaccc tgaaccgctt cggcgaccac gaccccgcag gcaagatgta
     2941 cgtcctcacc ggcatagacg gacgggcgac gaacggcgtc gcgaaggtac gacaggagga
     3001 gaccgctccg ctgccgggcc gcgtctccat cgggctgcac gggcacgacg cgatccagcc
     3061 gctcgtcatc cgcgtcaacc agggtgactg cgtgaagatc gacttccaca accaggcgac
     3121 cggcggggac tacggtgtcc acatcgacgg cctggcctac gacactggct cctcgggcga
     3181 ccagatcggc aacaacgcct cctcggcggt gccgaacggc gcatcgcagt cctacatgta
     3241 ctacgtcccg aacgacgacg cgctggaagg ctctcactat atccggcccg gtcccggcaa
     3301 ccgcgagtcc atctcgcacg gcctctttgg ttcgctgatg gtcgagcctc cgggctccaa
     3361 gtacctcgcc ctggacagcg gcaacccggt cgactccggc tgggaggcga gcatcgtccc
     3421 cggcggcgca acggccgaca ccagtgacga cgctccctcc ttccgcgagt tcgcgttgat
     3481 gcaccacgag ataggcaacg agaagttcaa gatcaagaac gtcgacggcg gcgacctccc
     3541 gaccgtggac ccgcacaccg acagctaccg gccgggctcg cgcgctctca actaccgctc
     3601 cgagccgttc atgaaccggc tggacctcga ccatgagcag aagtcgctct cgtacaactc
     3661 ctacacgttc ggtgacccgg cgaccccgat gccgcgcggc tacctgagcg atcccaccaa
     3721 gatccgtctc gtacacgccg gcacggagat gttccatgtc ttccatctgc acggcggcgg
     3781 cgaccgctgg cgcttcaacc cgctggcgga cacgaccttc aactacgccg acaccggcct
     3841 caacaaacac cccaagacga cccactcgcc ctccagcagg ctcgactccc aggcgatagg
     3901 ccctggcgag tcctacaacc tggagataga gggcggggcc ggcggcgtcc agcagtcggt
     3961 cggggacttc ctgttccact gccacatcgc caagcactac aactccggta tgtggagctt
     4021 ctggcgcgtc tacaacacgc tccaggacaa cttcaagccg ctgccggacc gcgtaccgtt
     4081 gccgaaggcg gttgactccg ccgggctcat cggcaagaac ttcaacggca ccacgataac
     4141 gaaggacaac ctcaagtcct gggtcgagca gcaagtccca acccagggcg tgccaaagaa
     4201 cacccaggac gcctcggtgt ggaactggaa gtgggacgat tcgaaccccg cggggtccgt
     4261 cgccctcggc gagcccgagg accagacgcc gtggccgaac catccgggcc tgctctggcc
     4321 ggacctcgcg gacggctcgc atcccggtgg tcagcccggc gaccagttcg tcggagaccg
     4381 tccaaagata atgttcgatc ctgtaagcgg acacccggcg ttcccactgc ttcggccaca
     4441 catcgcccag cgctcgccgt tcgcggggaa cggacactcc ggcacaccat acctgggcgc
     4501 gagtggagac caggcgccca acccgaacgc tccgggcggc gtggacccct acgccaaccg
     4561 cgacgacggc ctctgcccgt ccaagacggc cggaggagcg ccgacgcccc tcaggaactt
     4621 cgacatcacc tcgttcgtga cgccgatcca ggtgacggct aaaggcaaga cggaccagac
     4681 gggcatgatc ttcgccctga acaaggacaa ggcggctatc caggccggca acaagcctgc
     4741 ccagccgctc gccatccgcg gcaacatcgg cgactgcatc cagatcaact tcaacaccga
     4801 gctgacggat gccgcctctt tcggcaactt cgccaagggc aacatgcaca tccaccacgt
     4861 ccagttcgac atccagggct ccgacggcgc gtccgcgggc ttcgtctacg accagtcggt
     4921 ccgcccgtac caggccgtcg atccgcagct cacgcaggcc gcgaatgcgg gcgacacggt
     4981 cttgcacctc tccagcgtcg ccaagttcca gaacggagtc tggatcgagg tcggcgaagg
     5041 caccgataac ctggacgtgc gccagatcaa gtcaatagac tccgcggcca acacggtcac
     5101 gctcacccag ccgctcgcca agagccacgc gacgaacgag tgggccggaa ccgagttcat
     5161 gcgctaccgc tggtacccgg acgtggagct ggacaacatc ttctggcacg atcacgtcga
     5221 cggcatccac ggctggggca agggcctcgt cggacaactc atcatcgagc ccgaaggctc
     5281 cacctaccac gaccccaaga ccgggacgca ggtggactcc ggcaccatcg tggatatcca
     5341 ctcgaacaac ccgctggcgc cgggtgtccc cggcagcttc cgggagatgg cgctgtggac
     5401 catagacgag aacccggcca ccgactcgac gctcaacctc agggccgagc cctgggccga
     5461 caggctcgcc aacaacggcg acccgtcgct gttgtttagt tcctacacgc acggcgaccc
     5521 gaacacgccg cttccgaagg cataccctgg ggacccgttc gtgatccgca cgataaacgt
     5581 cagcggtggg gtcgacaccc tgcatattga tgggcaacgg ctcttcgtcg agaaccgcta
     5641 cgcggatgcc aacggcaaga ccgaggcttc accgctcgac accatccact acggcatctc
     5701 cgagaagtac acggcgatcc tcgaaggcgg cgccggcggt ccgaaccaca tacccggcga
     5761 ctacctgtac cagaacggta tcggacgccg ctttacccag ggagcctggg gcatcctccg
     5821 cgtcctggcc gggcagagcc ccgacctgca acccttgccg ggccatcccg cgccggccgg
     5881 aggcgggctg ccatcgccca acaacggacg tccgcccgcg gcctcgggac cgggcaaccc
     5941 ctgcccggcg ggcgcgccgc agcgcgactt caatgtcagc gccgtggacg tgcccggagc
     6001 cgcgcccgga agcgggcacg agctggcctt cgtgccgacc gctgccgggg ccgctgccgc
     6061 caacgagccg ctcgtgctgc acgcggcggc cggggagtgc atcaacgtga cgttcaagaa
     6121 cgagagctcc aacgaccgcg cctccttcca cgtcggcaag ctggagcgca ctattgagtc
     6181 gagcggtatc aacgtcgggt tcaaccccga gcagaccgtc ggcatcggcg gcacgcgcca
     6241 gtaccgcttc tacgccgaca ccgagaagat cgggagcgca gtcatcagcg acttcggcgg
     6301 caagccggtc gtccccgacc ccgttaccgg cgcgatcccg accaacgtgg ataccgggcc
     6361 acagggcctc tacggagcca tcgttatagc tcctcagggc gcgaccttca gcgacccgac
     6421 tagcggagcc gccacggacg tgggcaccca ggtggacgta catctgccgg gagcgcagcc
     6481 ctcaggctac cgcgacttca cgctcgcctt cgcggaggac gacccgcgca tcggccagga
     6541 cggcatgccc tacccgacag acgtcgccgg tcccgcgctc gtcaactaca cctccgctcc
     6601 gttgctcaac gatacgaacg cggcgttcag cggtaaccag ccgacgcctc tcttgcgggc
     6661 ctaccgggga gacccggtca aggtccacgc catcggcgcg ccgggcagcg agcagatgca
     6721 cgtgttcaac ctcggcgggc tctcgtggcc gatagacgcc tacatagaca acagcaacga
     6781 catgaccaac atgggcttcg gtcccttgga gagcatcgac gccgagataa acggcggcgc
     6841 cggcggacag tctcacacca cgggagactt ctactacggc gacatgcgcc gaccgttcac
     6901 ccaggccggt atgtggggcc tcttccgggt ctcctcggat gcgagctgcg cgcccgacgc
     6961 caagtcactc aagcctctcc ccggccgtga ctgcacgggc gctgtggacc acgacgcccc
     7021 gaccctgacc gccgacccgg gagcgggcgt gttcaacgct tcgcagcaaa tcaagatact
     7081 gtcctcggag cctgccaaga tccacttcac caccgacggc agcaagccga cccagcgtag
     7141 cccgctgtac aagacgccgc tcacggtgga caagtcggag acggtcaagg cgatcgcaat
     7201 agaccaggcg ggcaacagga gcgccgtagc ttcctgggac ttcacgatag acaccgtcgc
     7261 cccaaccgtg agcgcgaacc cgaagcccgg aacctacgac caggcccagc aggtcaagct
     7321 gcaggccagc gagtcgggct ccaggatctt ctacaccacc gacggttcga cgccgacacc
     7381 gcagagcacc gagctccagc ccggtacggc gatacctgtc agcgtctccg ggtccatcaa
     7441 ggccattgcg gtcgatccgg ccggtaacgc gagcgatcct gcgacattcg cctacacgat
     7501 ccggcaggca agcaaggtaa cgctcaacgt cgcgacaacg cccctgaggc tgggtaaggt
     7561 tcggaccata gcgggcgtgg tcagcccgtc ccacgcggga tcggtcaggt tgacgatcaa
     7621 gcgcaacggg aagacggtcc tcgccaagag cctgccgctg aagtcttcgc ggttcagcat
     7681 gccgtacacg ccacgcgctg tgggaagcta ttcggtgatc gtcaacttcg ccggggacgc
     7741 cgaccacaag ccgagcacgg cgaacaagag cttcaacgtg gtccggtagt catgatgcgc
     7801 cggtagtcta cggcgcggcc gcgagtctta tagagcctcg tccgaacagg acggggctct
     7861 ctctatctgg ggctggagag gtcctggatc agggccttga gcttcggacc gaggtcttct
     7921 atctcgtcag ccgtgcaact gatgtgcagg tgtacctgga ccttcgcgcc tccgccttct
     7981 tccccggtgg cctcgatgtc ctccccggcg actaccgcgg ataccgtttc cttggccggc
     8041 ctctcctcag gagtagcttc ttcctccggc tcgggcaact cgtctttcag gtcttcgtcg
     8101 aaggtggcga ccagttcgcc ggcctcctct ttcagcaggt tggcggcctt caggatctcg
     8161 atgagcgctc cggctccgct catgacgggt ttgttgcgcg gctgtccggc ggcgtgggcg
     8221 atgtacgcct ggacggtcgg gtagagcatc ccctccctga gcttcaccgc cgagaccacg
     8281 ttctgcagga actcgctcgt cgagaccacg gcgcgccagt tgtcgcggat accctcccgg
     8341 tccttcctcg cgagcgcgag cgccagcgac cttccccgcc gcgtgaccag cttctcccgc
     8401 tccccgcgca ggaccccgat gccggtcagg aaggcgttgt tgcggctgac gctcgactgg
     8461 tgcacggagt tgagcttgcc aacatcgccg gggttcgacg cctcgtcccg cgtcccgtaa
     8521 gccacgataa tgttgaccaa ctcctcgtag gtcgagcccg gcagcctgaa ctcttgcgcc
     8581 atccaacacc ttcccgaaga tcatccaccc gcagataaaa ctcgggaaca ttatcctaaa
     8641 tttggacggt ccatgctctg gcggcgcgtc caccccggcg tggactaact ccgggcacgg
     8701 cgtgaaccat cgtgagtacg ggctctttct atgtctcgcc gcgcaacctc gaccggggcg
     8761 ccggggttct ggatcccctc tccggatcac cctcgccggg ggctcttttt aaccaagatt
     8821 taagcgggat gtttccgtcc tttaaccacg ggcggtgtgc cggtccctag aatccggtgg
     8881 gtatggaagg aatcttttac acgacgagcg aggtgcgcga catgaccctg aagcagaagc
     8941 ccgacgctcc gccgatcacc tacccgtcgc ccgccgaaga tgaaccgcgt ccggccgggg
     9001 tcggttcccg ctggctgggc ttcgcgttag tggtcatagg ggtcggcctg atctctctcg
     9061 ccgccaccga tgccttcgcg ggcgccgcta ccggcggtgg ggctgacacg ctgcgcggtt
     9121 cggagccggg cgagcgcctg aatggatacg cgggcgacga ctacataaac ggctatgcgg
     9181 gcgacgacgc gctctcgggt ggcgatgggg cggacgaggt ctacggcggc cccggccgcg
     9241 atctcctttt tggcggcgcc ggggatgact tcattgaggc gaaggacggc gtggcggact
     9301 acgtggactg cggccccgga gacgacgtgg cgagcgtgga cggcgcggac cgcgcctcgc
     9361 cgagttgcga gaccgtctat cccggctgac cccgtgagcg gcgcaaccag gcattgaggt
     9421 tcggcgcgca cacgctgacg cggcgggact tcctgatgct cgcgggcgcg ggcgcgatgg
     9481 gcgcttccgg gctctcgctg gtgggcgcgc gggagtacga gaaggtaacg ggacgcagga
     9541 tctccctggg agaacagatg aacgtcataa tgatcgtcat agacagcctg cgtaaggatc
     9601 acgtcggggc gtacgggaac acctggatcc agaccccaaa cctcgacgct ttcgcaaagg
     9661 agagcctgcg cttcacccgc gcccagcccg aggccactcc gaccatcccg gcccggcgca
     9721 caatccatac cggcctgcgc acctggccct tcagggactg ggacccgcac aagggcgact
     9781 ccgtgatgct ctacggctgg cagcccatcc ccgagcagca gtggacgacg gccgagatcc
     9841 tccaggctca gggcttcgag accatgctag ttaccgacgc ctaccacgag gcgaaagccg
     9901 ccatgaactt ccagcgcggc ttcaacgtct tcgacatcat ccgcggccag gagaacgacc
     9961 acttccaccc gatgtggatg tgcccgcagg ataagataga gaacaccctc ctctccggcg
    10021 accggaggaa cgtggagggg aagatgcgcc agtacttcgc caacacgacc ggacgctccg
    10081 gcgagcaaga ctacttctcg ccgcaggtct tctcgcgcgc cgcgcgctgg ctcgaaggcg
    10141 ccagcaagca cggccggcca ttcttcatga gcgtggactc cttcgacccg cacgagccct
    10201 gggacccgcc ggagaagtac gccaagctct acagcgacgg ctacgccgga cccgagccct
    10261 acacctcggt ctacggcagc tcggactatc tggaagaagc ggagcttgat cggatgaaag
    10321 cgctctacgc cgccgagatc tcgatggtgg accactggct aggcaacttc ctcgacaaag
    10381 tggagagcct gggcctgatg gagaacacca tgatcctgct gctctccgac catggccacg
    10441 ccctcggcga gcacggcgcc gtcggcaaac ctccctacgc tctgtggccc gaactgaccg
    10501 acgtggcgtt catgatccgc catccggaag gcaaacgcgc cggggatgag agcgactact
    10561 acgcctccac ccacgacgcc gccccgacgg tcctcggcgc gctcggcgtg gaaccgccgt
    10621 cgcctatgga cggccaggac ctgatggtct tcttcgaggg cagggagccg gaggcgcggc
    10681 cgtacttcgt cggcgggctg cacgaccacc tctgggtgcg ggacggcgac tacgccatga
    10741 tctcgcgcaa caccggctcc ggcgcgaagc tctacgacct gaagatcgac cccacccaac
    10801 agaaagacat cgcctggcgg aaaccggacg tggtgaagcg catgttcgac gattacgtca
    10861 tcaaggcagc gggcggcgaa cctccgccga actacgacta ctgatcccgc aactccgccg
    10921 gacgcggttc cagcacgatg actccctcgt tctcgaaggc gacccggtag aggtctttct
    10981 gcgcgacgtg gccccgccag tcctgaaccg gcgtcctgtc ccaggtcgtg ccctcgccga
    11041 agcgcttgta gaagaccacg taccggacgt catgtttctc tagcagttgc cgcgttcgtt
    11101 cgtcgtccgg gtgttttatg acgtagagca cgtcgaggag cggtccggcg ccgtagggcg
    11161 ggaggtcgcg gtcgtagtgg atcctgcctt ccgtgaacgc ctccagcgcg aagtagccgc
    11221 tcagggcgag catcgctctc ccggtcacct ggttgccgcg cggggcgacc atgatgttcc
    11281 cgcccgtgtt atgcgctccg agccactcgc cggccgccac gatctgtggc gtcaggacca
    11341 ccctcgggct gggcttcgcc gccgcgtgca ggttccatac cgcctggacc ccgatcaccc
    11401 ccgccaccag cgccgccacg gccaccgccg cagccctcgc gagcatcccg cttccccgga
    11461 gagagcgtaa gatagccacg agcgcgaagg ccgcgaggag ggagagcggg atggcgaggt
    11521 ccctctcgaa gcgctcgggg aagccgctca ccgacgtgcg gctcccgacg aaaagtacga
    11581 ggcaccagag caggagtgtg cttcgggaca tggcggatgt caggggcccc tcgcgcccgc
    11641 cgagcatcag cagcgctccg agcaaaccga gccacagcac cggctgcgtg atcgtcttca
    11701 ggaagtgctc cagcgcgagt ggcgcctggg tcccgaccgc catcgagacc gcctggccac
    11761 ccgccgtcgt cccggagccg cgcaggagat cagcgaccgt ctgcggcagg ttgtaggtgt
    11821 cccaggcgta ggcggcggcg agcaggccca gcagcgcgaa cgagcccagc agcgcgaggc
    11881 cggtccgccg ctcgcgcagc agcaggtacg gcaggatcag cacgcccacc agcccgaaca
    11941 gcgccgccgc atagagactc ccgacgggat gatagagagc gaccgaagag ccgagcagcg
    12001 cgagcagcag cacggacctc gcggaaggtc tggcgtagag gccgacgagg gcggcgacgg
    12061 ccatcacgag caggaactcg gcggagacga gggtaggata catcgcctgg tagctcaggt
    12121 agataaaggt gctgttgcag accaggcccg atagtgcggc ggctgcgacc ccgtactccc
    12181 atccccacat gcgtcgcgcc agcgcgtaga gagccagggg tggcagcaga atcagggcgg
    12241 gcgtgtagac ggggaagagg gcgaccggct ccaggccgct gaggcgcgag atcacggcgt
    12301 ccagagtgtg cagccccggc ggatagaccg catatggctc tatcttccct ctcgtgagca
    12361 tcatgttcgc catcatcgcg tggaagtact ggtctccgcc gcgcaggtac ggccagtcgt
    12421 gcagaaccgg ccccgaatat ccccggaaca acacgagcaa cagaaccatc gggagcagga
    12481 gcctccgcat cgccggccac cggggctcca attcccgcga ggccgacgct accggatctc
    12541 ccctgcggac ctcgactgtg tacacgagcc cggcgaagag gagcaacagc gcaacagcca
    12601 acatcacctg ccgaccttgg agaatccccg aatacgccgc cgcaacaagt ccgaacgcca
    12661 gagcgagcag cgccagcgcg agcggaccgg ggggagtggt gaccggtatg atgggctcgt
    12721 ccagtccttt cggtgagcta aagatccggt acgccgcgag tccggagacg aagacggcca
    12781 gcaccgaaac gatggcgata gcgagggtga cgcccgttcc tgaaaacccg atgagggcca
    12841 gggctgtggc ggggacgagc gtgatggaga gcgcggtcga gaatgccagc ctctcggcgc
    12901 ggtctgcggt ggtggcgaag agcctcgacc agaagtagcc cggaacaact gccgcggcga
    12961 cggcggcgaa gagccctgga gtcagatcca gcagcataaa tacttcgcat ccggcgtatg
    13021 gttcacgccg gtaggttagc aagatgcccg gggatcgcgg cgccgccggg atggattaaa
    13081 cagaacgttt accaggattt agaatccatt taaccgaacg ccgggcgcgg ttctctagaa
    13141 tccgctgcta acgtttgttt gttaaagaaa tggagatatt gttaaagtac accaatgttc
    13201 ttgagaaacg tgcgagaata gagcgtcgcc agtgtccgtg tcagagtctc gcgcgggcgg
    13261 atacccgaaa aggcgaactc tcactgaatt cttagaaagg gcgagtaaat tttgatttcg
    13321 accgagacgg gccgaaaatc catatcctca aggaggtacg actgatgaaa gtgctggtac
    13381 taggtggcga tggttactgc ggatggccga cttccctgca cctgtctgat gcgggacacg
    13441 atgtggtcat cgtggacaac ttcgtccgca ggacgataga ccacgagctg ggggcgggca
    13501 gccttacgcc gatagccgat cccaaagagc gagtaaaagc ctggaaggaa gtaactgggc
    13561 agaccatcgg cctggagtac ggcgacctgc tcgactggga gttcgtatcg gagctcttcg
    13621 agcggcacaa gccggaagct gtcgtgcact tcgcggagca gcgggccgcg ccctactcca
    13681 tgatcgaccg tcgccacgcc gtctacacgc agcacaacaa cgtggtcggc aacctcaacg
    13741 tcttgttcgc gattcaggag tacgtgccgg agtgtcatct ggtgaagctc ggcacgatgg
    13801 gcgagtacgg ctacccgaac atcgacatcg aagagggcta catagagatc gagcacaacg
    13861 gccgcaagga cacgatgctc tatccgaagc tgcccggtag ctggtaccac ctctcgaagg
    13921 tccacgactc gcacaacatc cacttcgcct gccgcatctg gggcatgcga gctacggacc
    13981 tgaaccaggg catcgtctac ggcaccgtca cgccgcagac cgagcgcgac ccgcgcctga
    14041 tgaaccgctt cgactacgag gaggtcttcg gcacggccct gaaccgcttc tgtatccagg
    14101 cggccatcgg ccacccgatg acggtctacg gcaagggcgg ccagacgcgg ggcttcctgg
    14161 acatccgcga cacggtgcgt tgtatcgagc tggcggtgga caacccggcc gaccggggcg
    14221 agttccgggt gtttaaccag ttcacggagc agttcagcgt gctgcagctc gccgaaatgg
    14281 tcgcggacgc ctcggccaag ctcggcatcg aggcgcgcct ggagaacgtc ccgaacccgc
    14341 gggtcgagaa agaggagcac tactacaacg ccaagcacac gggcctgatc gagctcgggc
    14401 tggagccgca ctacctctcc gactccctgc tggattcgat gctcaacatc gccatcgagc
    14461 acaaggggcg ggtggatatg gacgccatca tgccgagtgt tgactggcgc accggcaagc
    14521 gtaccgcccg cgagcgtgcc gccgcgcccg ccgagtcccc ggccctctag cggaccgcta
    14581 acatacaggc gctaccgcgc ccccggagac tcggattaag agtttccggg ggctatcttt
    14641 tcgtctctcc gggcgcgaag atcacgacgc gcccgttctc gaagactttc ttatagtttg
    14701 agcttttctc gaaggtctgg aagtccatgc cgcgcggcgg cggtacccgc ttgtagagca
    14761 cgacgtagcg gacgtcgtac ttctcgatga gttgaagggt gcgctcgtcg tccgggtggc
    14821 cgagcatgaa gagcgcgtcc ttcagcggtt tcgctccgaa tggcggcagg tcgcgggcaa
    14881 gttcgatgcg cccggagtcg taggattgca ttcccgtgta accgccgagg gcgagaatcg
    14941 cgcggctcgg gaccaggtcc aggtaggggc tcacgaggat gttgccgccg gtgttgtggt
    15001 ctcgcagcca gttccccgcc gcctctatct tcggtgtgag catccgctgg gtggcggagg
    15061 tgatgagttg aggcgggatc tgggaggccg gtccgccggc tacgtcgagg ttcctgacgg
    15121 tctgaacgcc tatcagggcc gcggcgagca cggtggcgag ggaagtcgcg aagaccgtta
    15181 ccggcatacg gggttgcagg gagcgcagta gctggacgag cgcgaaggcg gcgagcaggg
    15241 agagcgggat ggcgaggtcg cgctcgaagc gctcggggaa gccgctcgcc gacgtgcggc
    15301 tcccgataaa gagcaggagc ccccagatga gcagcatagc gcgaccgaag gcgtatggcg
    15361 ggtccgtcct gccccggtcg agcgccagca ggaccgcgcc cagcgcccca aaccacagta
    15421 cgggagggga gatcatcgcc agcaggtgcg tgagcgatag cggcggctgg gtgccgatgg
    15481 ccatcttgag ggcgtcatcg cccctgccgc cccccggacc gccgaagagg ccggagatcg
    15541 cctgcggcag gtcgtaggtg tcccaggcga agacaacggc gagcactccc agcgccgcga
    15601 acgagtaaag catggcgagt ccgcgccgcc ggtcgcggag gagcaggtac ggcaggaagc
    15661 acagcgccgc gagcccgaac aggactatct cgtagagggt ggcgacctga tgatagagca
    15721 cggccgagga gccgagcagc gccagcagga cgccgctgcg ccgggtcggg acggcgagga
    15781 ggatcagcag tgcgccgacg gccagcgtga agaggaagtg tacggtgagc aggttggggt
    15841 agcgggcctc gacaaagtgc atgtacgagc cgtaggagac cactccggcg aagaaagccg
    15901 ccacgatccc agcttcccat ccccatatcc ggcgcgccag cgcgtagcag ccgagcgcca
    15961 cgagcgtgag cagcatcggc gcgagcaccg ggaagatctt catcgcgtcc atgccgctca
    16021 gttgcgagat cgtcgccatc aggtagtgga agccgggagg atagagcatg aagctatcgg
    16081 tggagccggt cgagatggtc atgttggtca tcacgacgtg ctcgtagtcg tctacgcccc
    16141 ggatgtacgg ccagtcgtag cgcaggggtc cgagataccc gcgcgcgagc gtcagcgaga
    16201 gcactacgcc caggagcagg taccggacga ccgtctccgg tttccgctcc ggctcgtgta
    16261 cggtgtcgtc ggtggttgat gcggggaccg taggtatgcc ggtgtccagc aggcgggcga
    16321 gtccggcggc gagtacgagc agcagggcaa gcggcccggt ccaggcggcc agcaccgcgt
    16381 gcgactcagc cgcgaacgcg actcccaggg cggcgatgag tgggatgagg gtcggtagcc
    16441 ccaggggagt tgagggtggc gctagcggtc cgtggggttt ctttaccgga ccgaacttga
    16501 ggcagaaagc gagccctccg ccgaagacga gcagcacgga ggagatggcg atgggtagtg
    16561 agagacccgt gccgaagagc ttcgcctgca agagccccat cgctgggact agcatcagcg
    16621 agagcccgat accgtagacg aagcgctcga ttcggtcggc ggtcgggcac aggcaggagg
    16681 cccacaggta gcccggaatc accccggccg cggtcgcggc ggcgagcgcc cggatcaggt
    16741 cccagatcat gtttcgttgt ccggtgcggt gctcgtttct ggcctccgtg tgacagtgtc
    16801 gaatcttcga gtacgtttta atcgctcagt cgggaaaatt cgagcaagtt taaccgaccg
    16861 tttaccagtt tgttgccaac atttaatatt cttttctata caatctccca acggcaagat
    16921 tccgcggggc gcgagcgcac tacgagccgc gcgccgcgag gaccggatag aattctcaga
    16981 gaggtgaact agatgagttc gaggagtaca ggttgacggc ggagcacgtg cggatagttg
    17041 gtatcgtcgt cgcgctgctg ttcgccggct atggcatcat caggtatcgc ggcggagagc
    17101 agagcaggtt cgacctgctg acggcgctgt tgatcgccgc cggggtggcg acggtctcga
    17161 ccatcccgca gatcggcgag gtcttcgtgc gtctgctcgg gttgcagaac cgcgcgttcg
    17221 cgctgctctc ggtggcgacg ctcttgctct tcgggctgta tctgcacctg ctgggccggg
    17281 tcagagaagc gagccggcgc aacggggaga tcgtcaccgg cctcgccgtc cgggagtacg
    17341 aggagaacta cctctccccg gaggagcaac tccccgaacg tctcgccgag cggcgcgaga
    17401 tcctgatagt agtgccggcc tatgatgagg agggcggcat ccgcgaggtg ctccgccgca
    17461 taccggatga ggtcctgggc taccaggtta agacggtcgt cgtggacgat ggatcgacgg
    17521 acgcgacggc tgccatcacc cgcgaagagg gcgtccccat cgtcacccac gtcgtcaacc
    17581 ggggccaggg cgacgcgctc aggacaggct ttgcgatagc catcaaggag gcggcggaca
    17641 tcgtgatcaa cctggacgcc gacggccagt acaagcccga ggagatcgac cgtctcgtaa
    17701 agccgatcgt cgaggacgag gcggacttcg tgcttggctc gcgcttcatg ggcttctacg
    17761 aggaggcggg gagcgtccgg cacgtcgggg tcgtgttctt ctcgcgcatg atctcgctgc
    17821 tcaccggcgt gaaggtctcc gactgtacaa acggcttccg cgccatcagg gtctcggagc
    17881 tgcccaagct aaacctgcgc gaagaccgct ttaacgccaa cgagatcatc ctggaagccc
    17941 tcaagaacaa gctgcgcttc acccaggtcc ccatctccat gatgagccgc gcggcgggcg
    18001 agaccaagaa gcccccgaag ctggcctacc cgctcggggt cttccgggtc atcatctcga
    18061 cctggctgcg ctagggcaac cccggctccc gcggctcggg gcgcgtaaga tagtcccatg
    18121 aagaacttcc tcagcgcggc taaaggacgg cagggagcgg gcgccgggag cctgtacatc
    18181 gcgttctcat tcgcggtatc cggcgcgctg accttcgggt tccagtcact cgcggcgcac
    18241 gctctcggga aggcgggcta cgcgccactc gcgctgctct ggtcctcgac tttcctggtg
    18301 gtgcaggtac tctggatcgg cgccacccag acgctcggtc gctacatcgc ggagcgggag
    18361 gcaaaggggc aggactggcg gcccgtgatc cgctctgtta agcgttggca gatcgggctg
    18421 tccggggcgt tcgtgctcgt ggcgctgccc ataagcccgt ggctgaccgg actcttcgga
    18481 gacccgtggc tcacggtggt gttcgtggtc tccatggtcg cctacgctcc ggagtacttc
    18541 cggcgcggca tcttcaacgg ccaccaccag tcctcgcggc tgggtgccca gatcctggcg
    18601 gagtcggcgg gccggttgct gatcgccgca gccctccttg ccgccgggat gggcgtgctc
    18661 ggtcccgcca tcgccatcct tctcgcgccc atcatcggcg tcctggccgt gcggcccgcg
    18721 cccgcggagc ctcccgagca cgagggagag cccttctccg ccatgaacgc cctcaagttc
    18781 gccgggccgg tgctaacgtg cgtggcgttc gcgcaggtcc tgatgaacgg cggtcccatc
    18841 ctcgttagcg tcctcggcgg cacaccggcg caggtggggc tctttggcgc ggcgatgatc
    18901 ctgacccgca tcccgcagta catcataagc cccgccattg gggcgttgct gccgcatgcg
    18961 agccgcatcc tctcgaccgg gggctacggg ccgttcggcc gcttcgtggg ccgcgccgtc
    19021 ggggtcgtgg gcctcgtcgg cgtcctgatg gtcggggcca cgtggctgct cggggagtgg
    19081 gggataaagc tcttcgccgg aagcgagttc gacgcgagcc gggagatgct ggtgacgctg
    19141 gcggcggtcg ccgccttcta cttgctgtgc gacatgctca accaggcgct cttcgccctc
    19201 ggccacgcac ggctcgcctc cctcggctgg ctcgtcggcc tggtcgtctc cgctctctgc
    19261 ctggctattc tggatacgga cgtcctctcc cgcgtctcct actccctcgc catcggcacc
    19321 ctcgccgccg cgatggcgca ggctgccttc tacctgatgg cgcggcgttc ggcgacggta
    19381 tcgagatccg gctaagaacg cgctatgctg gccgctccat gaaccagccg accgtgcggc
    19441 gcagtccgtc cggcagggcc acttccgctt tccaccccag catcttctcc gccttcgagg
    19501 tgtcggagta gttgcgctgc acgtcgccct gacgcggggt ggtctgccgg acctccacgt
    19561 cgtttatccc ggcgctcgcc aacacgggta ggagctcgtc tattagctcc cggacggtcg
    19621 tctcggtgct ggtggcgatc tggaatacct cgccacccac tccctcggcg gtcgcggaga
    19681 gcttgatcgc ctctacgaga tcgtcgatgt atatgtagtc gcgggtctgg gtgccgtcgc
    19741 cgtagatctc cagcgcttcc ccgttccttg cccgccggat gaacttggcg accgcgctgt
    19801 tcttgtgccc ggatagcggt ccgtagacgt tcccgaagcg caacgctacg gtatccacgc
    19861 cgaaagtgcg gaagtatgct gaacagtatc cttcgccagc gaccttgctc gcgccgtaag
    19921 gggagaccgg atgcgccggc agctcctcgt ggtagggcgg gtcaaccgcg ccgagcgcgg
    19981 ccccgctgga ggccgagacg aatctgcccg cgccgccgtg ccgggccgct tccaggtagt
    20041 tgagcgtgcc cacgatgttc gtcacgcagt cgtagcgcgg gtccttcacg gaaggctcga
    20101 cacccgtgtt ggcggctagg tgcaggatca catccgcccc ctccaccacc ctgagcgcca
    20161 gctcctcgtc catgatgtcc ccgaccacaa gctcgacacc agagacgatg ggaccgttcc
    20221 cctcgccctc cgagaactcg cagaccgagg ccaggtcctc gcgcgtgccg acggccagct
    20281 tgtccagcac ccggatgtcg tgcccgccct cttccagcag agattggatc accgccgtcc
    20341 cgataaaccc acacccgccc gttatgagcc acttcatgtt cagcccgcct ctcgtattgg
    20401 agtttttcat gagcggacag cataccccgc tagccacccg ctcgaaagcc cattcgtata
    20461 ccgttgaaaa aagaccaatc ggtctgttat tatagaaact gtggcataca gagtagtacg
    20521 aggaggtgcg aggatgagga aaggcgagag gacgcggcgc aggatcctag agcgctccgc
    20581 gccggtcttc aacacgaaag gctacttcgg ggcttcgatg agcgatctga tccgggcgtc
    20641 agggctggag aagggcggaa tatacaacca cttcgggagc aaggaggagc ttgcgctgga
    20701 gtccttcgac tacgcggcgg gaatcatgcg cgcccgcttc gacgccgcgc tggcggatag
    20761 ggagggcgct atagagcgtc tcttcgccgt agtggacgta ttaggcgcgc tcgtggaaga
    20821 tccgccggtc gcgggcggtt gcccggtgct caacaccgcc gtggagtccg acgacgccca
    20881 cccggcgcta aaagcgaggg ccgaagaggc gatgatcgga tggctccggc tcataggacg
    20941 cattgtcaag gacggcgcgc gggacggaga gctgaggact gatacggacc ccagagaggt
    21001 ggcgagcgta gtcgtggcta cgctcgaagg ggcgttgatg atcagcaagc tctgcgacga
    21061 cccttcccat atgcagcggg ccgtcgggca cctcaaaggc tatatagaga cactggctac
    21121 gcgggacgga cggagcgagg tgtagggtga aggttatcga ggaagactgg accgttgcgc
    21181 gagcagtctt tgcgcctggt gctgagagtc ccggcgtccc gcggtatgga ggcaacatcc
    21241 gggtccgggc cgtggagcgt ggagacctgg acgggctctg gcggatgctc tcccggagtt
    21301 cgtcggacac tatataccag aggttccacg cgccgtaccc gcgcgtgccc gaacgggtta
    21361 tggagttgct ggtagatgtg gatcctcgcg acgggaaggc gctcgtggcg gtcgcgggag
    21421 aagagatcgt gggtcacgcg atgtacgcgc ggacgcggga tgaagaagcc gagatcgcgg
    21481 tagtggtaga agacggctgg cagtcgagag gattgggacg gctcttgatc ttcgagatag
    21541 catcggtggc ccgcagcagg ggcgtcgagg tcctctattg ttcggcgctc gtcgagaacc
    21601 gccgcgcgtt agacttgtcg gcgttgatgt tcggtgaggt cgagtccgta tttagcggtg
    21661 gactgcggtg gatacgtgtg ccgctgcgtt ccctgaagga gaaggtatga ccgaaacgat
    21721 ggatcgcacg ctcacggtga gctgggagga ccccgcgccc ggagcggaag ctgcccggac
    21781 catgagtggc ctggagtacc tgcgggcggt ggcgaaggga gacctgccag cgccacccct
    21841 ggcgcacctc gtcggcttcg gcctccacga ggtcgaggag ggacgggccg tcttcgagtg
    21901 cgagcccggc gagcagcact acaaccccat cggcagcgtg cacggaggtc tcgcctgcac
    21961 gctcttcgac tccgccatgg gctgcgccgt gcacaccgcg ctcccggcgg ggaccggtta
    22021 caccacggtc gagatcaagg tgaacctgct gcgcccgatc acggtcgata ccgggaagct
    22081 tctctgcgag gggaagacca tccacgtcgg cgggcgcatc gccaccgccg aggcccggct
    22141 ctttgatgaa gctggcaagc tctacggaca cgccaccacg acctgcatgg tcttccggga
    22201 cgaggccaac ggaagttccg tcggagaggg gacgtcatga gggtcgcgcg cgtcgaggag
    22261 tttggcgggc cggaggtctt gcgggtcgcg gaggagcccc ggcccgagcc cggagagagc
    22321 gaggtgttga tcgagctgcg ctccgcgggc gtcaaccggg cggatgtcct caccaggagc
    22381 ggccgctacc acgccgccgg acagcctccg atagtcccgg gtttcgaggg aagcggcgtc
    22441 gtgcggaaga ccggcgcgtc ggtaacgggc gcgagcgtcg gggagcgtgt gatcgccttc
    22501 ggcgggcgtc cgggtttcta cgccgaatac gtcgccgtcc ccgaggagca cgtcgttgcg
    22561 atgccggatg gcctggatga agattcggcg gctgccctcc ccgtcgcgcc gctcaccgcc
    22621 ttctactgcc tgcggcgtct ggcgcgggtc gagccgggcg agacggtgct cgtatgggcc
    22681 gcggccagcg gggtcgggga tgtggcggtg caactcgcaa aggccgccgg ggcggaggtg
    22741 atcgccaccg ccggcaccga cgaaaaggtc gcctggaccc tggagaacgg cgcggaccac
    22801 gggataaacc actccatcga gagcgtcgtg gaccgggtcg tggagctttc gggcgggtcc
    22861 ggtgtggagg tcgtgctcga cacggtagga ggggcgcgtt tcggggagag cctaaaggtg
    22921 gccggccacg gcgggcgggt ggtctccctg gcgaacgtcg cgctggaagg aagcgcgctc
    22981 gacacccgcg acttctaccc caagaacgtg acgatctacg ggttccagat cacgaacctc
    23041 atgcaacgcc tcgggtacga tcccagggaa gacttggcgg agctggcaaa cctggtggtc
    23101 gatggcagtc tgaaggtaca cgtggaccgg gccttcccac tggaggaggc cgccgacgcc
    23161 caccgctacc tcgaagagcg ccgcaaccgg ggcaagataa tgcttcatcc tggggagggc
    23221 ggatgagcga cccactcggc gagttcccga aaccctcccc gcctcccgcc cccactactt
    23281 ccgacctctc cggcttcgag gagcgcgtcg agcaccgcta cgcggagaac gaaggcgtga
    23341 ggatccacta cgcggttctc ggcgaggggc cgctcgtcgt gatgatgcac ggcttcccgg
    23401 acttctggta tacctggcgg caccagatga acgccctctc ccaaaactac cgcgtcgcag
    23461 ccctcgatct gcgcggctac aacctctccg acaggccgga tagcatcgag agctacggga
    23521 tgaagtcgct cctgggtgac gttgcggccg taatccgggc ggaaggctac gagaaggccg
    23581 taatcgtcgg ccacgattgg ggtggcgcca tctcctggca gttcgcggag cgcctgtcag
    23641 ggatgacgga gaagctcgtc atcctcaacc tacctcatct caccggattg acccgcgagc
    23701 tggcgaacaa ccccgaccag cagcgggcca gctcctacac ccggttcttc cagcaggagg
    23761 gcgcccacga gtcgctctcc gccgggaagc tcgcccgctg ggtctccgac cccgacgccc
    23821 gcgagaagta cgtcgaggcg ttcgagagat ccagcttcga ggcgatgctc tactactaca
    23881 agcgtcacta cccggcggaa ccgtacaccg agctcgggcc gccgcaggct aaagtgccgg
    23941 tgccggtact cgtgatccac ggcctgcaag acccgtatct gttaccggat gctctatccg
    24001 gcacctggga ctgggtcgag agagacctga ccctcgtcac cgtcccggag gccggccact
    24061 tcgtccagca agacgccgcg gacttcgtca cgcggaccat cgaaagctgg ttggcacgat
    24121 gaagggtgag tctcgcggac ggctctatta cggctggatc gtggtcgcgg tcgtgttcct
    24181 ggctctgctc gtctcggccg gggtccgggc ggcgccgacg gtcctgatca acccgctcga
    24241 aaacgagctc gggtggggcc gggcgacgat ttccttcgcc gtctccatcg ggctcctgct
    24301 ctacggtctc tccggtcccg tcgccggatg gctgatggat cgcttcggac cgaagctcct
    24361 cacgctcgcc gggctggccg tgatcggcgg cagcaccctc gccggcgcga ccgtaaccga
    24421 gctttggcaa ctcaacctgt tctggggtgt cctgagcggc gtcgggaccg gggtcgtcgc
    24481 ccccgtgctc ggggccacgg tcgccaaccg ctggttcttc gagcgccgag gcctcgtcct
    24541 cggcatcttc ggggccgcgg cctccgccgg acaactcgtc tccgtccccg cgctgatgtg
    24601 gctcgtcgtt gctgtcggct ggcggcaggg cgtcgtcttc ctcgccctcg ccgcgctcac
    24661 ggtaatcgtc ccgatcctgc tgttcatgcg cgacgacccg cggaagatgg gcctccgccc
    24721 ctacggagac acggagtccg aggaacccgc tgctcagagt agtcccgctc cagaacccgg
    24781 aactccgctc gcggatgtgg acccgcagag gagtggcgtg ctgcggcggg cggtgcgcgc
    24841 gccggagttc tggctgctct cgggaagctt ctttatctgc ggggcgtcct ccaacggcat
    24901 catcggcgtc cacttcatcc cgcactcaat agatcacggc atcccggagg tcacggcggc
    24961 gggcgtgctg gcgctgatgg gggcgatgaa cttcgtcgga accatcgcct ccggtttcct
    25021 caccgaccgc tacgatcccc gcaagctcct cgccgtctac tactcgctgc gcgggctctc
    25081 gctactcttg ctgccgttcg tcacgcactt cgagggtctc gccatcttcg cggtcttctt
    25141 cggcctggat tacatagcta ccgtcccgcc gaccgtcgcc ctcgtcgccg accgcttcgg
    25201 caggatgaac gtcggcacgg tcttcggctg ggtcttcttc tcgcaccagc tcggcgcggc
    25261 gctggcctcc tacctcggcg gcgtcgcccg cgactctctg ggtgactaca ccgcggcctt
    25321 catcatcgcc gggatactcg ctggaatggc cgctttgatg gcgcttctcg tgcgccgcca
    25381 gcggccaccg gctgccggag agcctgtcag agcctagtca ggcgccctta gcgagtagga
    25441 actccgtgac ctgttggttg aaggtatcgg cccgctcgtc gtgaggtagc agggcggcgt
    25501 cgcggaagac gcggggggcg gtgcgcgggt tgtgggtcag gaaggtctgg agctcggaga
    25561 cgggtgtggt cttcgcctcc tggccccagc agatcatggt ccggtgcggc acccgcggcc
    25621 agtagtccgc aacgccgagg ttgagcttcc ccgccacgaa ggccgccggg aagtgcttcg
    25681 cccccggctg gtggctgatc cggtagtagc tctccaccag ctcctcagta acgaagctcc
    25741 ggtcgtggta ggacatgccg cccaggaagt accgcagccc cggccgcgag acgatggcgt
    25801 gatagagcga gtcgccgata acgggcgccg agaacagccc ctggataaag tcgccgagca
    25861 cgccggatgg acggtcgagg ctgccgtagc cggtcggaca gatcaggacc gccttcttga
    25921 agaggcgcgg gctgcggacg agcgccggca tcgccagggc ggcggtcagg gaactggcga
    25981 taaggtgcgc cgggttcccg atctcctcgc gcgcgaagtc ctctatctgt gagcttacgt
    26041 cctccggctc gtagcgacgc cgcggccggg ccgagagccc gcaccccagc aggtcgagcg
    26101 catacacccg gaagctctcc gacaactcca ggaagttctt aaggaactcg aacgatgacg
    26161 ccccggcgta caccccgtgt accatcaaca gcggtggtcc ctctcccgcg accgcgtagg
    26221 cgaggtcctc gcccttccac cggaagtacc gggtctcgcc cccgatcctg ttcccgatct
    26281 ccccgccgct ccgcgcgagc cgttggttgt aaagcgccag cccaccgacc gcgcctcctg
    26341 tggcgagcaa cctcccagat ttcatgacac cctcccgatg aaatttcacg ttgacaactg
    26401 gactatatac gatattgttt aaggcgttta aataagtgtc ttaatgaaat aggagatcgc
    26461 ttggaccggg agcagctcat agacgaggtc ttgatgttgt tgccggcctt gatgcggctg
    26521 gtcgggcggc cggggccggt cgagatgggt gagatcgcgc gtcgcgggat accggtggac
    26581 gcgcacatct cgccggggca cgtgcagatc ttgatctctc tctcgcgcgg gccgcggtcg
    26641 gttggacaac tggccgagga cgtgggggtt tcgccgccgg ccgccaccca gcttatcgac
    26701 aagttggtag agcatgggat ggtcgagcgg cgccacgacc acgcggaccg gcgggtggta
    26761 ctggtggact acgtgccggg gatgcacgag gtcgcgcggc gcatcatgga agaccggcgc
    26821 aaaccgatag agcggaccat aaactcactc accgacgagg aggcccgcgc cttcgtcaag
    26881 ggactcaagt taatgaccga aggtctcggg acggggccgg tggagagttg acctgtggca
    26941 gcgagtacag aagtcgtgga gagacgctat atggtcctca agtctgacga gggatccatc
    27001 tcaaggacca tgtgccgttt gttcgtgcct tttacagtaa cgtcgtgtcc cgcggaccag
    27061 gaagggaacc cggtcaaggc cgcggatact gcttttacgg aacctagctt cggcgtggcg
    27121 ccgggggagg agagttgatg gagactatcg tccgctggtg cctcaagaac aagtcggtgg
    27181 tcatcctggc ggcccttctc ctcataggag caggggcgta cgcgaccacg cgtctgaatc
    27241 aggagttgtt gccggacatc tcgttcccgg tcatcgtcgt ctcgacgccg gtctccgggg
    27301 cggggccgga cgtggtggac gagcaggtca cgcagccggt cgagagcgcc ataaagggta
    27361 ttaccgggat cgagagcgtc cagtccacct cctcgcaggg cttctcggtc gtggtagtac
    27421 agttcaacct cgacaccgac acggaggagg ctgaggcgga cctgcagtcg gccctcgacg
    27481 gaatacagct cccgtcacag gccgggacac cggaggtcaa gagccagtcc gcatcgcagt
    27541 tcccgatcct caacctctct ctggccgcga aggaccgcga cctcgccgac ctcaccaaat
    27601 atgccgaaga cgatgtcgtg cccgccatcg aagaggtgga cggcgtggcg agcgttgacc
    27661 tgatcggcgg cgccgagaaa cgcatccagg tggatctcga cccgaaaaaa ctgaaggaga
    27721 agggcctctc gaccgaggcc gtcgtcggcg cgataagcgg cgccgaggtt gactccccgg
    27781 tcggcagcgt tcccgtgggc ggtctcgaca cacccatccg cgccaccagc aagctcggcg
    27841 gtgtgaaagc cctcgaagac ctgcccgtgg gagtcgaagg cggatcggcc agcgccgcac
    27901 cgaccggcac ggcctccagc gcgtctacaa acgtacctgc tggtataccc gctgggatac
    27961 ccgctggcgc aacaggcgct gctccttccg gctcggcgtc tcccagcggc gcccccgcag
    28021 ccgggactac ggctgctccc accgctacgg ccgctggaga ccaggcgcca aagccggtgt
    28081 tgctgggaga tgtggcggag gtgcgcgagg tcggctccga cctctctggg atctcacgga
    28141 ccaacggtga gccgagcctg gggctgaatg tcatcaagga gacggacgcc aacaccgtcg
    28201 aggttgcggc ggatgtcgag aaggtcctcg atgacgcgcg ggataagatc ggtgacggcc
    28261 aggtcgtggt tgtctccaac tcggcgacgg acgtcgagga atcggtgaac gggcttgtcg
    28321 aggaggggct cgtcggggcg gcgctggcga tactggtgat cttcgccttc ctgcgctccc
    28381 tgcgcgcaac actcgtaacc gccgtctctc tgccgacatc ggtgctggcg gcccttctgt
    28441 tctcctgggc ggacaacctg acgctcaaca tcatcaccct cgccggcctg actatcgccg
    28501 ttgggcgggt cgtggacgac gccatcgtcg ttctggagaa ctcgtaccgc tacgttcaga
    28561 gaggctacga gccggaggag gcggccctca aaggaacgac cgaggtggcg agcgccatca
    28621 cctcctcgac cctcacgacg accgccgtct tcctcccgct ggcgctggtt ggcggcatca
    28681 tctccaagtt cttcgtcccg ctctcgctga ccgtcgccct ggcgctcctg gcgtccctga
    28741 tcgtggccgt caccatcatc cccgtgctag tcagcatctt cgtcaagcgc cgcgccggag
    28801 actacacccc ggatgcttca gcggacgagt ccgacgatga gccgctacgg agagtggata
    28861 tgcgcagccg gagaggtggc ttggcacgct tcctcgtagg ggtagtagtg ttcctgatcg
    28921 cctcggcggc tgcggtcgtt atcgcctcgc tggccggcgt gctcgggagg ctccccggtt
    28981 tgccgcagag ctacgccgaa gccgtggatg gtttcgtctc cggggtcggc ttcggctccc
    29041 cggtcttcct ggcggtagtt ggggtggccg gggcgttgct gctcgcgggg ctcgtgctgc
    29101 tcgtcgtgcg ggcggcgcgg cgtccgagcg gcggggagtc ggacggcgtg ctggtccggc
    29161 tctacactcc gatgctgcga tggagcctgc gacaccggtt ggcggtgctg gtgctggcgt
    29221 tcctggcgtt cgccggcggg ctcgggctcg tgcaactgct cccggtcagc ttcttccccc
    29281 cgagcgagga gcggctcctg atcgccgacg tggaactgcc tgcgggcacc agcctgcaga
    29341 agacgagcga caggctccgt ccgttcgagg atttcctggg caaagaccgg ggcgtaaaga
    29401 gctaccaggc ctccatcggc ggagaagaca ccctcgatcc ggagtcgccc gtccggcccg
    29461 gtaacaaggc ccaggcgttc atcaacgtga aagcgagcgc caacgtcggg cgcacgctcg
    29521 acaggatcga cgccgaggga gacgacctct acggcgagaa cttcaagata caggtcatct
    29581 ccaatggccc gccccagggc ggtctcgaag ccatcctcac cggcggctcc aagaaagacc
    29641 gggccgacgc cgcggacatg gtctccaaag agttcaacaa gctcgacaac gttaacaacg
    29701 tcgagagcga cctctccggc ggcaaccccg aggtccaggt aacagtggac cccgagaagg
    29761 ccgcgaaggt cgggctctct ccgggaggtg tctccagctc gctcggcgcc ctgctcggcg
    29821 gaagtaccgt gacgacgctc ggcgacacgc cggtcgtggt cggggcgccg gcgaagaccg
    29881 ttgactcgct ggacgaggtg cggaagctat ccgtcgggag cggaagcacg gtcggagacg
    29941 tggctcaagt agatcaggta gagtcaccgg cggccgtcag ccgcaccgat ggggaccggg
    30001 cggtgacggt gacgggcgag atcaactcga acgataccca gactatctct accaaagccc
    30061 aggctgccgt ggcgaagctc gacctgccgg gtaacgtcaa ggctcaggtg ggcggcgaga
    30121 ccgacgacat agacgagagc ttcaggaacc tcttcctctc cataatcgtg gcgctggtgc
    30181 tggtgttcct gatcctggtg gtcttcttcg gctcgctcct gataccgatg gtgatcctgc
    30241 tggccgtgcc cctcacgacg gtcggtgctt tcggcgcgct gtatcttacg aacacggcgc
    30301 tctccgtccc gtccctgctg ggcatcctgc tcctcatcgg gattgtggtc tcgaacgcca
    30361 tcctgctggt ggacttcgcc gtcaaggccc tggaccacta cgagacaccg gacgaggcca
    30421 tcctggcggc gggccaggcg cggctccggc ccatcctgat gaccgctttc gcgaccgtct
    30481 tcgccctgat gccgctggcc ctcggtctct ccggcggggg caacgggctc atctcgtcca
    30541 gcctcgcaat aacggtcgtc ggggggctcg cgacctccac gttcctgacg ctgctggtcg
    30601 ttccggtcgg tttctctttc ctgaagagcg gcctgaggcg caagaagaag gtgtagctgt
    30661 tggagcagaa gatcgaggca ggagaccgtg cgccggggtt cagcctgctg gcggcgggct
    30721 ccgggcggcg gttgagcgtg cggaaatcgg aggggcgttc gctcgggctc atcttctacc
    30781 cgcagggcgc ggctccggcg gcgcggaggt caaccgggcc gtccgcgccc gttatccgtc
    30841 cacggacgac gttctgatcg ccagcgtcat agacctctcc atcgtgccgc ctgtctaccg
    30901 gatggcggtc gggatggtcc tgaacacgtc ctacgagcag gcggccgaag agatccctca
    30961 agacatgaac ccggcggagt acgtgatcaa cggccccgac tacggcggcc ttatgagccg
    31021 ccgctacggc gtccgcaaca ccggccgcgc cgccgccatc gtggatggag actcgaacat
    31081 ctccggctcc taccagggcg aacggcctgt cgagaacgtc tttgagatgc tggatgcgag
    31141 gtgagtgtta gagacgacag tcgtgtgagg ctgtcggctg cacactgaag tcaggaagac
    31201 gcggagagaa aggagcatgc cataggcagg cagagacatc gccggaggac gcccgaccgt
    31261 cagaaccagg gccgcatgcg cgagtccctc aggcagaggc tcgaagacgg cccggcccgc
    31321 atcgcccgcg aggaggtgga ggagatcggg gctgatatcg gcatggagcc cgtggacgcc
    31381 gtgcggctct tcgaccgcct gaagggcgtc tcgtggcgcg gcgactacgt cgagtccgac
    31441 ggaggacgct gggccgccgt ctgggtgaca gaggtcaact agactttatc ctcgcgcgtc
    31501 caaactggac tctcgcgtgc cgcgggtccg ggatcgtggt aaaaggcccg tatggacgtt
    31561 cgggaagaga gaccgttcag agaccgggcc gacgccgggc gaaggctcgc cgggaggctc
    31621 gaaaattacc gcgatgagga gccattggtc ctggcgttgc cgcgaggcgg ggtcccggta
    31681 gggttcgagg tcgcgcgcgc cctcggtgcg ccactggacg tgtttgtctc gcgcaagctc
    31741 ggcgcaccgg gccagccgga gctggggatc ggcgccgtcg cccagggcgg cacgcgcgtc
    31801 ctgaacgagc gcctggttcg gcggctcggc atcccggacg agtacctgga gtacatcacc
    31861 cgccgggagc aggccgaggt cgagcggcgg ctgcgcgccc tgcgcggcga ccggcccgaa
    31921 ccggaggtcc gcgggcgcac ggcgatcctg gtggacgacg gccttgctac cggggtgacg
    31981 gcgatggccg ccatagaggc tctcaagaca aggaacccgc ggaggatcgt cctcgccgtc
    32041 ccggtctgcg ccgctccgac ggcggagatg atgcggccgg aggtggacga tctggtatcc
    32101 ctccgtatcc ccgaggacct ctacgccatc ggcttctggt acgaagactt cgagcaggtc
    32161 ccggacgaga ccgtgatcga gcttctgaag cgagcccgcc gtgaagggga gagagacgac
    32221 cgggagggat aacttgcacg tctacaccga tgccgccatg agagaccacg tgaacgggct
    32281 ctctcacgtc gaggtcccag agcggttccc ggcggcaatc gccggagtcg aggcggcgga
    32341 gtccgccggc gtcgccgtgg agtggcgagc gagcgatccc gccccggaag aggcgctcct
    32401 cgcggtccac gagcggtcgt acctggagaa actggagcgg gtctgcggca cgggcggcgg
    32461 gttcctgggg acggacaccg ccgccgggcc gggctcctgg gaggctgcgc tgctcggcgc
    32521 cgggtgcgcc gtgggggcgg tggagagcgc gctatctggc gagaccgcgt tcgccgcggt
    32581 ccggcctccc ggacaccacg ccggacgcgc gaccgcgatg ggcttctgtc tcctcaacaa
    32641 cgccgcgata gccgccgaac acgtccgctc gcttgggaaa gacaggatcg ccatcctcga
    32701 ctgggacgtt caccacggca acgggaccca ggacatcttc tacgaggacg gagagatcct
    32761 gtacctctcg gtacaccgca gcccattcta ccccgggacc ggcgccgcga aagaggtagg
    32821 agcgggcgaa gggcggggca tgaccgtcaa cgtcccgttg ccgcgcggca cgggcgaagc
    32881 tacttacgct gtggcattcg ccggagtcct cttgcctata ctgcgcgcgt tccgtccaga
    32941 actcctgatc gtctccgccg gatacgacgc tcacgccgac gaccctctcg gcggtatgcg
    33001 cctgcaagcg gaatccttcg gtcgcttcgc ggccacgctc gatgccctcg tccgcgagat
    33061 aggagcggct ccgctcgtga tgctgctcga aggaggctac gatctcgccg cgctcaccgg
    33121 cagcatcgcc tccacactaa gaggaatcgg acaggagccg ccggactgga acatggaagg
    33181 tgatcctgac gatcctgagg tcgagaacag ccgcgaagcc ctggagccct actggcacag
    33241 tctacggtaa ctaccagcaa gcaaaagcgc cgccggaatc tccggcggcg ctcggttcgt
    33301 gcggttggag gctggtgtta cgcggcgacg atatccagga atcggccgta ctcgccggca
    33361 cggatcatct gctccgcctc gcgctggagc tggcggatgc gctcgcggga gacgcccaac
    33421 tcgtcgctga gctgcgcgag ggtcgcgggc tcctgcgcgt cgagaccgta gcgccggatc
    33481 aggacgtgcc gctggcgctc cgggagctgc tcgatagcgg cgccgagccc ttcggtctcc
    33541 atctcgcgca cgactgcccc ggcggtgtcg gaggcgcgct cgtcctctac cagctcgccc
    33601 agctcggagg cgtctccgtc ggagctgagg ggctggttga ggctcgtggc gtccggcatg
    33661 gcgctcttca cgtcgcgcac ctcgtcggca gtccagccga tcctctcggc gacctcctcg
    33721 tcggacgggt cgcgctcaag ttcaccggag agctcggtgt aggcgcgggc catcttccgg
    33781 atcttctctc ccatgtgcac gggcacccgg atggtgcggc ccttgtccgc gacggcccgc
    33841 tgcacggcct gacggatcca ccacgtagcg tacgtggaga agcgccaacc ccgctcgggg
    33901 tcgtacttct cgaccgcccg catgagcccg atgttgccct cctggatgag gtcctcaaag
    33961 ggtagaccca tcccgcggta cttcttcgca acgctcacca cgagccgcag gttcttctcg
    34021 atcagcttct gccgcgcccg cgtatctccc gccttcgcgt tccgcgagag ttcgatctct
    34081 tcggcgtgcg taagcaggtt gcccttgtct atccgggcga agtagcccgg tatgagatcg
    34141 ggggtgtccg catcgcgttc ccttcgccgt gtcaccatag tctccatata tcaacctccg
    34201 tctgtctctg atcgccggac gcttccccgc ccggcacttc cgtcaaacgc cgcaaaaccc
    34261 tcatacccga taccctccgg gcaatgatta ccaagttgag ctatcctggg atctgaaagg
    34321 atttgcttgt caacattctc gtcaacctga taatcatata cccaaattcg cgtttgtcaa
    34381 cacctcggag cgagtaaaca gcgtattatt ctgtgctgag gcgcagggtt ccggccttct
    34441 cgctgcggac ttctaccttg acagggccgt cgccacgcac gacccatacg atgcgccggg
    34501 cgtagtctga gggcaggccc acgaagaaag aggagtcttt ccaggagttg ccttcgagtg
    34561 aggaccggcc ggtgaggtgc ccgagatcca gctcaagatc gccggagacg agagtcgctc
    34621 ctttcggcag ctctatctcc gccttgacgg tctggactag cttcttgtcg gcggcgatgc
    34681 gggttacgtt ggtcggcagg tagccggtgt tctggacgcg cagctccagc cggcgcagtc
    34741 cgggcgagac ttcttcggct tgcagggtgg cttcgaggcg cggggcagcc tcggcgttgg
    34801 agagagcgaa gcgtgtgagc gcggcgcact cggcttcgag gaacttcggc ggcgggttct
    34861 gggcagtgaa cttggtcttc cagcctccga tctccacccg ccccagttgc gggtggtcga
    34921 acggccgcca gtctacgaac ccttcgccgt ccagatcccg gtcgttccat gcgagcatct
    34981 ttatctgggc gtcctcgggc gggcggcgga tgaactcgat aaagtccttg acctccacgc
    35041 ccgctgcctt cgcggccgac cagaactcga cggtgaaggc gaagacgccg taggagtcgt
    35101 aggcccagtc gtcgaaggtg ccggtgatga acttggtcgg ctcgtagcgg aagtcgtggt
    35161 agacggagat gttcgggtag ccggtaaggg cggtcccccg ctcgccgagg gccttataga
    35221 cggcgaggtc gtgatcgggc atctccaggt catcgcggtt ggagtaggga cgaaggatca
    35281 cgccggagaa cgtgtgatag gtgtgagcgg agaagacgtt cttgcgcgcc agcagggcgt
    35341 ccacgtgcgc cctcgactcc ggctccgaga gcgggtacgg ccccgcgccg cgctggtcgt
    35401 catctccctt ccagtggtac gggtactggc ggttcatgtc cagaccgtgc aggggccgcg
    35461 ctatcttccg ctcgaagccg tcgtagtcct tgaaatagcc ctcgcggtag atgcggtagt
    35521 aggtggccct cgggtctgcg tcgtccgggg cgcgggggag catcaggcgg gggtctttgt
    35581 cggagacgcg ccattcgccg tgagggtcct cgacgcgcat ctggaggatg tggccgtcgc
    35641 cgtccacgtc ttcggcgtgc aggccgggtt cctcctccgg ctcgggccag gggcggaggg
    35701 tggcgcggag ggtgtagggg gtggtcaggt agcgttcggc gccgtcgggg gagaggcggg
    35761 ggaggatgta gaaggtctgc tcgtccagga ggcgggtaat cttttcgtcc gaaccgtggc
    35821 cttcgaggag ggtgtggatc aggtacaggg cggacatcga gccggtgact tctccggcgt
    35881 gggtattgcc gtccacccag aaggcgggct tctcggaggc gggaccggtt gcctcattgg
    35941 ttagctccgc cagccagatg tcgcggccct cgtagctctt gcctacggag gttatggtgc
    36001 agaggtccgg gtgctcccct gcgagcgcgt ggagggtgtc ggtcaactct tcgtagcgca
    36061 gataacggtc gaaatctatc ttcatacgcc ccttctgatt gcttcgcggg ccaaacatga
    36121 gcattatgga acatttttcg ggacgcgaga ccgcaaaatg tagtggggta acgtgttaat
    36181 ctgcagcatg ttgggtgccg tcgcggtagc tacggccctt atgtatcgag tgaacggcga
    36241 gggatatata attgcggcac gagcggtata cggttgaagg cggtgaagtt ctggacgcct
    36301 cggttcgcgt cctcgggggg cgctacagga tacagggtac gctaggaacc ggggggatgg
    36361 ccgtagtcta caaggctgag gacgccatcc tcggccgcaa ggttgccttg aagaccctgc
    36421 acgaccggta tgcggaggag cctgccttcc ggcggcgctt caagcaggag gcgcgggcga
    36481 tggcctcgct ggaccatgag aacatagtca aggtctacga catctcccag gacggcgaca
    36541 acccgtttat cgtggcggag tgcgtcagcg gcaaggacgt tggggacctg ctggcgcaga
    36601 acgagggcgg acctctcaac gagcagttca cgcggcgggt agcggagcag ctcctgcgcg
    36661 cgctctccta cgcgcaccgg cgcgggatca tccaccggga cataaagccg tcgaacctgc
    36721 taatcacgca cgagggcacg gtcaaggtgg cggacttcgg tatcgcgcgc atcgtcgagg
    36781 acgaggatgc gggggagccg ggggagatcg tcgggagcgc ccgctacatg tcgccggagc
    36841 agctcagggg tgaggaggca atcccggcga gcgacatcta ctccgtcggc atcctgctct
    36901 atcattgcct cacgggcgag ccgcctttct cgggcgacgt gaagagcctg gcccggcagc
    36961 acatgaagag cgatcccacg cccccacgcc ggctgaacaa gaagatctcg cgtcatatgg
    37021 aggccgtgat cctgaaggca ctcgccaagg acccgaagga ccgttacacc tccgcggacg
    37081 tcatgctcga cgacctggag gccgaggggc catcccggaa ggctatcggg acgcgggaag
    37141 ccccgaagcc cgccgccgtg agcaaggctc cgaagaaggg caagagcagg cgcagggggc
    37201 tgcttctgat cccggctctg gtgctgttga tcctcgccgc aggaggaggc gctttcgcgg
    37261 cggggcttgt ggacgtgcct tcgaagcaga gcattcagca ggcgttcggc caggcgcagg
    37321 aggtcgtgtc ggatcccata gaagcgccga aagcttcttc ggccgacaag gcttcgcagc
    37381 ccgtcaagga cgataggaag gatgaggtct ccgcggagat ggtcccggtc ccggacgtgc
    37441 gggtgtacta tgattactcg gcgcgggaga tcctggtgaa ccgcggcttc aaggtccgga
    37501 tcgtatatag tcctcaggaa ggctatgcgg aacgtggggt ggcctggggt acgaagccgg
    37561 ccatcggaga gatggccccg aagggctcca cggttacagt ctacgccacg cccaagaacc
    37621 tgtatcaacc tcagatacgg agttgatcct ggtccgtaac ggcccctgct agaaggtcct
    37681 tcccgtgcct tccatcctgt ccgcgatgta ggggtagcgg aagtcggcgc ccgtatagac
    37741 cctgtaggcg gcgtagaggc tgtgcacgaa ggggaccagt cccaggaccg ccgcgagggg
    37801 tatcaccagg aagagaccca ggccgagggt cacgatggag aacaacgtgc ccaggatgcc
    37861 tccaacggtc gcgatggcga gccaggccaa ctggtaccag agcgattgca gggcgtggaa
    37921 gccgacctgc ggcgagcggt cccggtagac gagccagatg atcaggggcg ctatgggcag
    37981 cagccccgtg gcgggccaga cgaggatact cgcatgggag agcgcggacc agacgcgctc
    38041 atcctgcggg ctcaaagctc cgtccgtggg ctcctcggcc ctaccgtagt tgcgtggatc
    38101 ctgctgcatc catgatcctc ctgtaagagt caacccgcta gatatattcg tcgcctcagt
    38161 gtcaccgagt ggtacagcac gaggatcgcg accggcaacg ctccctgtac ctccgcatcg
    38221 aagacagcct tgtagcggcg gcccgcgagg ccaccggaga gccgcgctac ctcgctaacc
    38281 tcctcgctac catccagcgt gtgtactacc gtaacgttgc tgagcagccg cagccgcgct
    38341 tcgtacgcct gacccgcgca ctcaactcgc agtacggtgg cgcttcctcc gggagacgcg
    38401 gcccgtgcca gctcggtccc tcccgagagc atcctgtatc cgccgcgcga gtccccttcg
    38461 atctcgatct caaggcttcc ggccgtgagt gttacccggc cgccccgcgc tcgcagctcg
    38521 gcgaacggag tcccgctccg gtccgtcagg gtataggacg cgcggaggag accgcgagcg
    38581 cgggcggcga aagagccgtc gagacgatcc tggagcatgt tccaggggat gagttctgga
    38641 gcttgtgtag aagagcgcat ctcgtcctct aatacgcggg aggggtgttg gagtttcggg
    38701 aggagagcgg ttacattacg gggagtcgag taggaagaga gcgccgatgg aactcaagaa
    38761 caaagaggag atccgaaccg agatcttgca gtcctggcta aggaatgcgt gggtataccc
    38821 gcagcagggc gccggcgagc agactttcct gcgcctgacg cccggcggac ggctcaagat
    38881 gcgccgcaag atcggcgagc tggaaacctc tctgggcgtg agcggcggcg agctggcgaa
    38941 gcaggaagag gccgggaccc tccccgtaga gcgcgacaga atggaactcg ccatgatggt
    39001 tcaggcatac acctccgagc ggaaatttat ccagagccag ggcgcgaatc ttggcactcc
    39061 ggccgttgaa ctagaaggcg aggagccgga gagcggtccc gaggaaacct aggctgtctt
    39121 gcggttcagg cggcgttcta gggcgttggt caggtagatg aggggcaggc agatcgccag
    39181 gtagatgagc gccgccacga cgagcggcgt ggcgttggcg gtagcggatt gcaggacccg
    39241 cgcggcccgc agcagctctt cgaggccgat gacggagacg atggacgtgt cttttatgag
    39301 catcacgaac tcgttggtga ggggcgggat ggtgcgccgg aaggcctgcg gcacgattat
    39361 gtgacgcatg gtctgggcgt aggagaggcc ggacgcgcgc ccggcctcca tctggccctt
    39421 atctatgctc tggatgccgg agcggaagat ctccgcgacg taggcggcgc tgttgaaggt
    39481 cagcgcggtg agcccggcga agaagaggct ctggaagcgg atgccgataa ggggtgtcgc
    39541 atagtagatg aagatgatct gtaccagtag cggcgtgccg cggaagatgt ctatgtagag
    39601 ctgcgccggg aagctgatta tccgggagcg cgagatcttc aggagagcca gcacgaggcc
    39661 gaacactatg ccgaacacct ccgccaggag agccagcacc agcgtcacca ggacggcctg
    39721 aacgataacg tccaggttct ggaacgcgag tccgaagtcc aggtatttct cccggatatt
    39781 ctccaggctg aagttgctgg ggtcgaactc taccggcaac cactcgggca tctggctctc
    39841 ccactgctag agctaacgga ccttacggga actctttacg ggatctcctt gggctccgtg
    39901 ccgatccact tcttatagat cttcgcgtag gtgccgtcgt ccttgatctc cttgagggcc
    39961 ttgttggtcg gggccacgag ttcgctgtcc ttggggaagg ctatgccgta ctgctcgccg
    40021 gtcgggatgg tctgcacgat cttgagcgct ccgccgctct tgttggcctt gtcctgggag
    40081 acgggcaggt cgtttatgac agcgtccacc tggccgttct ccagcgccgc gaaggcgtcc
    40141 tccaccgtgt cgaaggtccg cacctcggag atcttgccct gggacttgaa ctcctcggct
    40201 ttcgaggctc cggtcgtccc gatctggacg ccgaccgtag aatccgcgag gtcatccacg
    40261 ctctttatgc cggagtcgct ggctacgagg agcgactggt cggcgttgaa gtaggggtct
    40321 gagaagtcga cctgctgctc gcgctccggg gttatggtca tcgcggagat ggccgcgtcg
    40381 tagaggttgg agccaagccc gccgatgatg ccgtcgaagg tgacgttctg gtactcgggc
    40441 gtgaaaccgg cgcgcttgcc gatctcgttc atcaggtcta tgtcaaagcc tttcggctcg
    40501 ccgccctcgg agaactcgaa cggccggtag gcgatgtccg aggcgacttt tacggtctgt
    40561 ccgctcgccg cgccttccag cccactcgtc gtgccgcccg ctccggtcgt ctcgccgcca
    40621 ccgccgcctc cgcaggccgc aacgaccatc gagagcgcga agacgagcgc aaccagcatc
    40681 acaccattta atctcttcaa ttcttccttc ctttcataaa gtttccaaac ctcggccagc
    40741 gtatcggccg gaagcggttg atccctccgg tagttaataa caaaattact catcagcgca
    40801 agtcaggccg caaaggcccg actacatacc ctcgcgttgc ccggcgtccg gggccgtgct
    40861 acggtgcggc gttgccggcg acggagggag tatgtagccg tgagagaacc cgatagcgcg
    40921 ttcgtcagcg ccgtaacgcg ggccgtcgag gaccgtcgcg acgatgcggt acggctgctg
    40981 cgagcgttag tgaacatacc ttcggtgacg ggcggagagg gagccgtcgc ggaagcagtc
    41041 gcgcacgcct tccgggagcg cggcctggat gtggacgtct gggaggcgac ccgcgaggag
    41101 atggaacctt acctggagca tgtcggggag caggagacgt atgcgggccg cccgaacgtg
    41161 gttgggagac gggcgggctc cgg
//
LOCUS       DASSMB010000010        12728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1015897, whole genome shotgun sequence.
ACCESSION   DASSMB010000010 DASSMB010000000
VERSION     DASSMB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12728
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015897"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFJ72_00855"
     CDS             complement(<1..886)
                     /locus_tag="VFJ72_00855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7478041.1"
                     /translation="MDQRNLGSEGLVVSELGLGCMGMSEFYGTTDEDESIATIHRAIE
                     LGINFLDTADMYGPFTNEKLVRKAIADRRDQVVIATKFGNERGDDGSFIGVNGKPEYV
                     RSSCDASLKRLGIDHIDLYYQHRVDPDTPIEETVGAMKELVDAGKVRYLGLSEAGPGT
                     IRRAHAVHPISALQSEYSLFTRDVEDEILPTVRELGVGLVPYSPLGRGFLTGRFKSIE
                     DMPENDTRSARFPRFARENFEKNVKLAGRIREIADDRGITPGQLALAWVLAQGDDIVP
                     IPGTKRRKYLEENAGAADV"
     gene            1085..1291
                     /locus_tag="VFJ72_00860"
     CDS             1085..1291
                     /locus_tag="VFJ72_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478042.1"
                     /translation="MTKAFQVTMPEGGAHTFDSFECAIHVLAPSCEHCGVKVIGHGVE
                     AGGSFYCCAHCASMAGGSGVRDRA"
     gene            complement(1298..1657)
                     /locus_tag="VFJ72_00865"
     CDS             complement(1298..1657)
                     /locus_tag="VFJ72_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478043.1"
                     /translation="MDRYSFDVRLTPKARTMLKPGEAVVLDWHRLAMCCAGAGESSLY
                     VTSEESARGRKGLVRLKGEDQIYAARAIFPHLAGREVLIDARKTLGFRRFVSNLPADF
                     GLRAVMGRLPEPERAAR"
     gene            complement(1670..2665)
                     /locus_tag="VFJ72_00870"
     CDS             complement(1670..2665)
                     /locus_tag="VFJ72_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7478044.1"
                     /translation="MEYRRLGNTGLMVSELCLGCMTFGRETDEAGSREIVARFLEAGG
                     NFIDTADVYSEGVSEEITGRAIKSVRDDVVLATKVRFPMGDGPNDVGLSRKHITRGCD
                     ASLKRLGTDFIDLYQVHCWDAATPLEETLSALTDLVRAGKVRYIGVSNFTAWQLAKAM
                     GVSELRGFERFVCLQPQYSLVERNIEREILPVCREEGLGIIPWSPLGGGFLSGKYRRH
                     EKPPEDSRIAAAEEPMEEHWSRRATGRNWRVLDAVGEIAEASGKSYAQVALNWLLRQE
                     GVTAPIIGARTIEQLEDNLGATGWELTGEQVRKLSEAGALEDTYPYRFIKNAQRI"
     gene            2771..4423
                     /locus_tag="VFJ72_00875"
     CDS             2771..4423
                     /locus_tag="VFJ72_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7478045.1"
                     /translation="MRSTKLWRDQLQVSDVVGTRGTRGSRTALAAALACWVAGALLFA
                     AFPEGSGARSITALGVSWAAMIFAGLALAAALSRAGRSERLFWMLMGGGVALRFAGDL
                     LWTMSRFLDVDTNAALAPQDVAYAISYSLFFGALLRLVSLTTRRVTLVSALDAASIML
                     SVGTLAWYFVLGPGAAEAGLDNLRDALVALFQPVCDAALLYLGLVVMSNSRRPSFVGP
                     LIGGFLAFLIADTMYLGLRSDGPYDLGSWPDLFWALGIVLVGVAAAATLSAPAPLAPQ
                     HRIAPWRVLTFWLGPLSPPLQFGVMLLWGAFNPPLPAYIPAGAAVLLLYLAVRIGLIS
                     FVSRSLSEEQEALARELEQSRILYELHDTVKQSIHGVSLTLTSALEAERRGDHDAARE
                     MFGRALETSREAEFQISRPYDELQSLRRETPPRAGDFLCQRLRKFEEYFGVKTHDDLQ
                     APLEVLSPAQIAALTRVFVEALWNVAKHSKAQNLYLESRRVGSLLMVRIRDDGRGFDT
                     EDPSPGLGLEYMRRRAGEVGARLDVISTPGRGTTVQIRFDRT"
     gene            complement(4437..6167)
                     /locus_tag="VFJ72_00880"
     CDS             complement(4437..6167)
                     /locus_tag="VFJ72_00880"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HET7478046.1"
                     /translation="MRVALAQINTTVGDVWGNAEKAAGALERALEAGADLIAFPELTT
                     TGYPPEDLLMRPNFIEENLQALDELAARVPEGVAAAIGFVDLDADLYNACAVVSGGEV
                     LHRYHKRYLPNYGVFDENRYFREGTGAPVVRLGGALVGVSVCEDIWYPGGPAREQALG
                     GASVLLNISASPYHRSKGAFRERMLGVRASDYGCYVLFCNLVGGQDELVFDGHSVIYD
                     PEGELLARADQFREDLILADIFPEQSLIQRLHDPRPRKENPDSPPRVVEIPASVGKGE
                     PVEPVEYRVEPLLGEEGEVFEALVLGLGDYFGKNGFSRAVLGLSGGIDSSLAAAVAVE
                     ALGPENVTGVLMPSRYTSDLSNTDAAALAKNLGIDTQTVAIGPAFDAYREMLAGAFKG
                     LPEDVAEENLQSRIRGNILMALSNKFGWIVLSTGNKSEMSVGYSTLYGDMAGGFAVIK
                     DVPKTLVYSVCRHINELKGREIIPDSVLTKEPSAELREDQRDTDSLPPYDILDPILEA
                     YIEEDRSIGEIVAVGFEEADVRRVVQLVDRAEYKRRQAPTGIKVTGRAFGRDRRMPIT
                     NRYSERSGEE"
     gene            6166..7188
                     /locus_tag="VFJ72_00885"
     CDS             6166..7188
                     /locus_tag="VFJ72_00885"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HET7478047.1"
                     /translation="MRPIYRARPSAAVYATAAATALATALAAASGAGCAAGVEWFAGT
                     AVSRDTGEDWREVAKNDLPAGETRRRVESARRKTTPWIERYARFGYVAYGLVYALVGF
                     FAVQAAFSGGGKTTGQEGALQSILSAPLGRVLLVLIAVGLLGYAAWRLLQGIMDPDDD
                     GSDAKGIVKRLDHGLNGLFHGALALTAGRLALGLGGGGGGGSPDGWTARLLAQPFGRW
                     LAVIAGVIIVGVGLYQFYKAYKASFRDELKLHEMSLREKIWATRSGRLGYAARGVVFG
                     VIGVFIIQAAVQTDPQKARGLGGALQTLARQPFGPYLLGAVALGLVSYGVFMLVMARY
                     RRIQTP"
     gene            complement(7201..8106)
                     /locus_tag="VFJ72_00890"
     CDS             complement(7201..8106)
                     /locus_tag="VFJ72_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7478048.1"
                     /translation="MFPTKIMVATDGSVESERAVRMAVTLSDRLDSELHVLHVGHIPN
                     VYTAPEATIVDPEFHEKMREIGERDAREQMREELEKIEKMGGRVADHHVEVGRTDARI
                     VAVAEEIGAGLVVVGSRGLGPLRRALLGSVSQSVVRHAHGPVLVVRDGGMERDYLPGR
                     VLLALDGSREAAAAARAAVQIAGATASELHLLFVLHNDLGMPPAHVLTSERSEAILHQ
                     ARHDARQFIDRQAENLEAEGAKVKDAHLAFGRADEEIVRLSEELEAGLIVMGSRGLEG
                     VRRSLLGSVSDSVVRHAHCPVLIVR"
     gene            8341..8820
                     /locus_tag="VFJ72_00895"
     CDS             8341..8820
                     /locus_tag="VFJ72_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478049.1"
                     /translation="MSFPDSQPEASAHSSAHSFVTDGVRAAGALEEACRRSLEETQSL
                     HLEVVRDLAADLRRQLDALSGMVEERAEPRVDAALACADLATLAACNVIQMPPAEART
                     VANAVHIAAGAAHSLSGTAENISKDEAEYLARDARGARWKADLASRQADEFLSSRRS"
     gene            complement(8817..9446)
                     /locus_tag="VFJ72_00900"
     CDS             complement(8817..9446)
                     /locus_tag="VFJ72_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7478050.1"
                     /translation="MDERIDILDEWGRPTGEVAWKSEAHRLGLWHRCFHCWIAGSDDD
                     GPYLLVQRRAAVKDTWPGYLDVTAAGHLGTGEEPVAGGLRELEEELGLRVDPARLIPL
                     GTRRVEQEIPAGRDREFHEVYILQDSTPPGDLRLQREEVASVVRVGLGDAERLLPGRT
                     APAREFADGGSGAITVSLSNFVPHEDDYLRRVAQASLALLAGESVGRPF"
     gene            9544..10578
                     /locus_tag="VFJ72_00905"
     CDS             9544..10578
                     /locus_tag="VFJ72_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET7478051.1"
                     /translation="MTTVVIASYFEEEHVRRIREVDGRARVLYREDLVPPPRWDGDHR
                     GIDGWERTPEQDEEFLAMLAEAEVLLDFPRGHGRELTKVAPKLRWLQGSMAGAGEPAR
                     RAGLIESDIVVTTAGGVYSGPLAEFVLLALLSHVKDFDRLRSDQRERRWGERYVETLE
                     GKTLCIVGVGSIGRAIARRARPFGMRVLGVKRTVREEDPAREETDELYETARLREALG
                     EADYVAATLPLTPQTERLLDAGTIAAMKPGAYFVNVGRGKVVDEAALVEALRSGHLSG
                     AALDVFEEEPLPGENPLWGMQNVIVSPHSTDNVPGLTNRLLTDLFCENLRRYLDGEPL
                     KNELNKRLLY"
     gene            10736..12067
                     /locus_tag="VFJ72_00910"
     CDS             10736..12067
                     /locus_tag="VFJ72_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7478052.1"
                     /translation="MIFPALRILGALSLVVLNGLFVAAEFSLVKIRATQVDRLVEEGR
                     TSAGIVKEATGKLDAYLAVCQLGITISSLALGALGEPAIASLIEPVLEPLGIAGGTLH
                     LIAFIIGFGIITFLHVVFGELAPKSIAIQSAEGTSLFVAPFMKFFYYLLLPATTVFNG
                     TANLFIRMLGYGSASESDETHSEGEIRTLLTQSAQQGEVEEDEEDMISAVFELNDKSA
                     REIMVPRPDTVSLPADLPLRKLVSVAAAGNYTRYPIYEDDSPDRIIGMVHVKDVLRSV
                     DEKGLEAEITARDLMRGVLIVPENRPIDQILEDFQDQEIQMAIVIDEWGSFEGLFTIE
                     DILEEIVGEIRDEFDEEEPAVRKLSDGSYSVDGRIPISVVNEALGSEFESEDFDTIGG
                     LVLGSLGRVPDTGDEVTLDGYALRVDEVDGTRVAQVIIREAPPEESGETGE"
     gene            complement(12079..>12728)
                     /locus_tag="VFJ72_00915"
     CDS             complement(12079..>12728)
                     /locus_tag="VFJ72_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HET7478053.1"
                     /translation="LDWEKYRSRPRFDSGLDLADPALYPVRASGTEARVPAMHLKSAV
                     ISERRDTRVAAENGLVTLDGHDDLLHAALVDREGAWVSRALVSGFAANLDGLASTYNT
                     TTQLLVLGRRPEAMALAARRGRDLNGGIVIVEGREIVYELALPITGMMADLPFEEVVE
                     RNRRLSETVSAAGYEHHDILYTLLFLTCDFLPALRLTPLGLLDVKSSEVLIPAGR"
BASE COUNT         2074 a         4213 c         4167 g         2274 t
ORIGIN      
        1 tcacgtccgc ggctccggcg ttctcctcca ggtacttgcg acgcttcgtg ccggggatcg
       61 ggacgatgtc gtcaccctgc gcgagcaccc aggcgagagc gagctggccg ggggttatgc
      121 ccctgtcgtc ggctatctct cttatgcggc ccgcgagctt tacgttcttc tcgaagttct
      181 ctctggcgaa gcgcgggaag cgggcgctgc gcgtgtcgtt ctccggcatg tcctcgatgc
      241 tcttgaagcg tccggtgagg aagccgcgcc cgagcgggct gtatggaacg aggccgacgc
      301 cgagttctct cacggttggc aggatctcat cctccacatc ccgcgtaaag agcgagtact
      361 cggactgcaa ggcgcttatc ggatggaccg cgtgcgcccg ccggatagtt cccggcccgg
      421 cctccgagag ccccagatag cggactttac ccgcatccac cagctccttc atagctccga
      481 ccgtctcctc gatgggcgta tccgggtcca cgcggtgctg gtagtagagg tctatgtggt
      541 ctataccgag gcgcttcagg ctggcgtcgc aagagctacg gacatactcg ggcttaccgt
      601 tcacgccgat aaaactgcca tcatcgccgc gctcgttgcc gaacttggtg gcgatgacga
      661 cctgatcccg gcgatccgct atcgccttac ggacgagctt ctcgttggtg aacggaccgt
      721 acatgtccgc cgtgtccagg aagtttatgc ccagctctat agcgcggtgg attgtggcga
      781 tggactcgtc ctcatcagta gtgccgtaga actcgctcat ccccatgcac ccgagcccca
      841 gctccgagac gaccagacct tcgctaccga ggttcctctg atccactacc tatccttccg
      901 tcgagaactc cttgcggggc atgagtttac tgcaactacg gtttcggaga gtttccatca
      961 cgatactcga tgaccgatcc tcgcaggttt cgatccccac tccccggaaa atttatcttt
     1021 ccgagggaaa gcgaacggga aaggagcgta acgatggcgc agtgcgaagt ctgcggaaat
     1081 gactatgaca aaagcgtttc aggtgactat gccggagggt ggcgcccaca ccttcgacag
     1141 ctttgagtgc gcgatccacg tcctcgcccc atcttgcgag cattgcgggg tcaaggtgat
     1201 cggtcacggc gtcgaggctg gcgggtcttt ctactgctgc gcccactgcg ccagcatggc
     1261 cgggggaagc ggagtccggg accgcgcctg agactctcta gcgggccgcc ctctcgggct
     1321 ccggtaggcg gcccattacg gcgcgcaggc cgaagtccgc gggcaggttg gaaacaaagc
     1381 gccggaatcc gagggtcttg cgagcatcga tcaggacctc gcgtccggcc aggtgcggga
     1441 agatggcgcg ggcggcgtag atctggtctt cccccttgag ccgcacgagg cccttgcgtc
     1501 cgcgggcgct ctcctcactc gtcacgtaga ggctcgactc gcccgccccg gcgcaacaca
     1561 tcgccagccg gtgccagtcc aataccacag cctcgccggg cttgagcatc gtccgcgcct
     1621 tcggggtgag tcgcacgtcg aatgagtacc tgtccatgca acgagtctat catatgcgct
     1681 gggcattctt tataaagcgg tatggatacg tgtcttcgag agcaccggcc tcggagagct
     1741 tgcgcacctg ttccccggtc agctcccagc cggtcgcgcc gaggttgtct tctagttgct
     1801 ctatggtgcg ggcgccgatg atcggggctg tgacgccctc ctgacgtagc agccagttga
     1861 gagcgacctg ggcgtaggac ttgccgctcg cttccgctat ctcaccgacg gcgtcgagga
     1921 cgcgccagtt gcgcccggtg gcgcgccgcg accagtgctc ctccatcggc tcctcggccg
     1981 cggcgatgcg cgagtcctcc ggcggcttct cgtggcgacg gtacttgccg gagaggaacc
     2041 cgccgccgag agggctccac ggtatgatgc cgagcccttc ttcccggcag accggcagta
     2101 tctcccgctc tatgttccgc tcgacgagcg agtactgcgg ttgcaggcag acgaaacgct
     2161 cgaaaccccg aagctcgctc acgcccatcg ccttcgccag ttgccaggcg gtgaagttgg
     2221 agacgccgat atagcggacc ttgccggctc gcacgaggtc ggtgagcgcg gagagggtct
     2281 cttcgagcgg ggtggcggcg tcccagcagt ggacctggta gaggtctata aagtcggtgc
     2341 cgagacgctt gaggctggcg tcgcagcccc gtgtgatgtg cttgcgggag agtccgacgt
     2401 cgttcgggcc atcgcccatc gggaagcgga ccttcgtggc gagcaccacg tcgtcgcgga
     2461 cgctctttat tgcgcggccg gtgatctctt cggagacgcc ctccgagtag acgtcggcgg
     2521 tatctatgaa gttacccccg gcttcgagaa agcgggccac tatctccctt gagcccgcct
     2581 cgtccgtctc gcgcccgaac gtcatgcacc ccagacacag ctcggagacc ataagacccg
     2641 tgttgcccag ccgtctgtac tccatcaaac ccctctctaa aagcgctcgc cggaagatct
     2701 cactctagca ttggacgtgg ttgaggggta tcttggggcc gggcagcctt gcaagaagtg
     2761 gattgatagg atgcgttcga cgaagctctg gagggaccaa ctgcaagttt cggacgtagt
     2821 cgggacgcgc ggtacacgcg gctcaaggac cgcgctggcg gcggcccttg cctgctgggt
     2881 tgctggtgct ctgctcttcg ccgcgttccc cgagggctcc ggcgccaggt ctattacggc
     2941 gctgggcgtc tcgtgggcgg ccatgatctt cgccggtctc gccctggcag ccgccctgtc
     3001 gcgcgccggg cgatccgagc gcctcttctg gatgttgatg ggcggcgggg tggccctgcg
     3061 cttcgcggga gacctcctct ggaccatgtc gcggttcctg gatgtggata cgaacgcggc
     3121 gctggcgccg caggacgtag cctacgccat ctcgtactcg ctattcttcg gagcgttgct
     3181 gcgcctggtc tcgctcacga cgcgcagggt aacgctggtg agcgccctcg acgcagcgag
     3241 cataatgctc tccgtcggca cgctcgcctg gtacttcgtg ctgggtccgg gcgcggcgga
     3301 ggccggtctg gacaacctgc gggacgcgct ggtcgccctc ttccagccgg tctgcgacgc
     3361 ggcgttgctc tacctcgggc tcgtggtgat gtccaactcc cgcaggccca gcttcgtcgg
     3421 gcccctgatc ggcggtttcc tggcgttcct gatcgcggac accatgtacc tcgggctgcg
     3481 ctccgacggt ccctacgacc tgggtagctg gcccgacctc ttctgggccc tgggcattgt
     3541 cctcgtcggg gtggcggcgg ccgcgacgct ctccgcgccg gcgcccctcg cgcctcagca
     3601 ccgcatcgcg ccctggcggg tactcacgtt ctggctcgga ccgctctccc cgccgctcca
     3661 gttcggggtc atgctgctct ggggcgcgtt caatccgcca ttgcccgcct atataccggc
     3721 gggcgcagcg gtcctgctgc tctacctggc ggtgcggatc ggcttgatct ccttcgtcag
     3781 ccggagcctc agcgaggagc aggaggccct tgcccgcgag ctggagcaga gccgcatcct
     3841 ctacgaactg cacgacaccg tgaaacagag cattcatggc gtatccctga cgctaacctc
     3901 agctctcgaa gccgagcggc gaggagatca tgacgccgct agggagatgt tcgggagagc
     3961 tctggagacc tcgcgggaag ccgagttcca gatctcgcgc ccctacgacg agcttcagtc
     4021 cctgcggcgg gagacgccac ccagggccgg cgacttcctg tgccagcgcc tgcggaagtt
     4081 cgaggagtat ttcggggtaa agacccacga cgacctgcag gctccgctgg aagtcctgag
     4141 cccggcccag atcgccgccc tcaccagggt cttcgtcgag gctctctgga acgtcgccaa
     4201 gcactcgaag gcccagaacc tctacctgga gtcccggagg gtgggctctc tgctcatggt
     4261 ccgcatccgg gacgacggcc gcggcttcga cacggaggac ccatccccag gcctcggtct
     4321 cgaatatatg cgccggcggg ccggcgaggt gggggcacga ctggacgtca tctccacccc
     4381 cggccgcggg accaccgtcc agatccgctt cgatagaacc tgaagctctc ggtctactac
     4441 tcttccccgg agcgttcgga gtagcggttg gtgatgggca ttcggcggtc gcggccaaag
     4501 gcgcggccgg ttacttttat gcccgtgggg gcctggcggc gcttgtactc ggcgcggtcg
     4561 acgagctgga cgacgcggcg gacatcggcc tcttcgaagc cgaccgccac gatctcgccg
     4621 atgctccggt cctcctcgat gtaggcttcg aggatcgggt cgaggatgtc gtagggcggc
     4681 agcgaatctg tgtcgcgctg gtcctcgcgc aactcggcgg aaggctcctt cgtcagtacg
     4741 gagtccggga tgatctcgcg ccccttcaac tcgtttatgt ggcggcagac gctgtagacc
     4801 agcgtcttgg gcacgtcttt tatgacggcg aacccgcccg ccatgtctcc atagagcgtg
     4861 gaatagccga cgctcatctc gctcttgttc ccggtactga gaacgatcca gccgaacttg
     4921 ttcgacagag ccatgagtat gttgccccgg atgcggctct gcaagttctc ctccgccaca
     4981 tcctccggta atcccttgaa ggcgcccgcg agcatctccc ggtaggcgtc gaaagccggc
     5041 cctattgcga cggtctgggt gtctatcccg aggttcttgg cgagcgccgc ggcgtctgtg
     5101 ttgctgaggt ccgatgtgta gcggctgggc atgaggacgc cggtcacgtt ctccggcccc
     5161 agcgcctcca cggccacggc ggcggcgagc gaggagtcta tgccgccgga gagaccgagc
     5221 accgcccgcg agaatccgtt cttcccgaag tagtccccga ggccgagtac gagcgcctcg
     5281 aagacctcgc cctcctcgcc gagcagcggt tcgacgcgat actcgacagg ctcgacaggc
     5341 tcgcctttcc cgacgctggc gggtatctcc accactctgg gcggactatc ggggttctcc
     5401 ttgcggggtc gagggtcgtg caggcgctgg atgagggact gctccgggaa gatgtccgcc
     5461 aggatcaggt cctccctgaa ctggtccgcc cgcgccagca gctccccctc cgggtcgtag
     5521 atcacggaat gcccgtcgaa gacgagctcg tcctgtccgc cgacgaggtt acagaacagg
     5581 acgtagcatc cgtaatccga cgcccggacc ccgagcatcc gttcccggaa ggcgcccttg
     5641 ctccggtggt agggtgacgc cgagatgttt agcagcacgc tcgccccgcc caaagcctgc
     5701 tccctggccg gtccacccgg ataccagata tcctcgcaga cgctcacccc cacgagcgcg
     5761 ccgccgagcc ggacgaccgg cgctcccgtc ccctcccgga agtagcggtt ctcgtcgaag
     5821 acgccgtagt tcggcaggta gcgcttgtgg tagcggtgca gcacctctcc acccgagacg
     5881 accgcgcagg cgttgtacag gtcagcgtcc aggtccacga agcctatcgc ggcggcgacg
     5941 ccctccggga cgcgcgcggc gagttcatcg agcgcctgga ggttctcctc gatgaagttc
     6001 ggccgcatca ggaggtcttc cggcgggtag ccggtcgtgg tgagctccgg gaaggcgatc
     6061 aggtccgctc cagcctccag cgcccgctcc agggcgccgg ccgccttctc ggcgttaccc
     6121 caaacgtcgc ctacggtcgt gttgatctga gcgagagcga ccctcatgcg cccaatatac
     6181 cgcgcccggc catccgctgc tgtatacgct accgcggcag ctacggcgct cgctaccgcg
     6241 ctcgctgccg cgtccggcgc ggggtgcgcc gcgggtgtag agtggttcgc cgggacggct
     6301 gtgtcccgcg ataccggaga ggactggaga gaagtggcga agaacgatct acccgccgga
     6361 gagacccgcc gtcgcgtcga gagcgccagg aggaagacaa ctccctggat tgaacgctac
     6421 gcccgtttcg gttacgtggc ctacgggctc gtgtacgcgc tggtgggatt cttcgcggtg
     6481 caggccgcct ttagcggcgg tggaaagact accggccagg aaggcgccct ccagtccatc
     6541 ctgagcgcgc cgctggggag ggtgctgctc gtcctcatcg ccgtcggcct gctgggatac
     6601 gccgcgtggc gcctgctcca gggcatcatg gacccggacg acgatggctc ggacgcgaag
     6661 gggatcgtga agcgcctgga ccacggactc aacggcctgt tccacggcgc cctggccctc
     6721 accgcggggc ggctggccct cggcctcggc ggtggcggcg gtggcggctc gccggacggc
     6781 tggaccgccc ggctcctggc ccagcccttc ggccgctggc tggccgttat cgcgggcgtg
     6841 atcatcgtcg gcgtcggtct ctaccagttc tacaaggcat acaaggcttc tttccgggac
     6901 gagttgaagc tgcacgagat gagcctccgc gagaagatct gggccacgcg cagcgggcgt
     6961 ctcggctacg ccgcgcgcgg cgtggtcttc ggcgtgatcg gggtcttcat tatccaggcc
     7021 gccgtccaga ccgacccgca gaaagcccgc ggcctgggcg gcgccctgca gaccctcgcc
     7081 cgccagccct tcggtcccta cctgctcggc gccgtcgccc tcggcctagt ctcctacggc
     7141 gtcttcatgc tcgtgatggc ccgctaccgc cgcatccaga cgccctgacc cgcgctagcc
     7201 ctaccgaacg atgaggaccg ggcagtgagc gtgccgcacg acggagtccg agacgctccc
     7261 caagagcgag cgcctcacgc cttcgaggcc gcggctcccc atgacgatca ggccggcctc
     7321 cagctcctcg cttagccgga cgatctcctc gtccgcccgt ccgaaggcga gatgagcgtc
     7381 cttgaccttc gctccctcgg cctccaggtt ctcggcctga cggtctatga actggcgcgc
     7441 gtcgtgccgg gcctgatgaa ggatcgcctc ggacctctca ctcgtcaaca cgtgagccgg
     7501 cggcatcccc aggtcgttgt ggaggacgaa gagcaggtga agctccgagg cggtggcgcc
     7561 cgctatctgg accgccgccc gcgccgctgc cgcagcctcc ctggagccgt cgagcgccag
     7621 aagcacccgt cccggcaggt aatcccgctc catcccaccg tcccggacga cgaggaccgg
     7681 accgtgagcg tggcggacca cgctctggga gacgctcccc aggagcgcgc gcctcaaggg
     7741 acccaacccc cggctgccga caaccacgag cccggccccg atctcctcgg ccacggccac
     7801 tatcctcgcg tcggtgcgcc cgacctccac gtgatggtcc gcgaccctgc cacccatctt
     7861 ctctatcttc tccaactcct cccgcatctg ctcgcgggcg tcgcgctccc cgatctcacg
     7921 catcttctcg tgaaactccg ggtccacgat ggtcgcctcc ggcgccgtgt acacgttcgg
     7981 gatgtgcccg acgtgcagca catgaagctc cgagtccagc cgatcagata gcgtaaccgc
     8041 catccgcacc gcccgctccg actctacaga cccgtccgtc gccaccatta tcttcgtcgg
     8101 aaacatcacc cacactcctc gtctcgcctt tcccactatc attctaaact ctaagtgttt
     8161 tagtatcaaa tatcacacga catgcatgcc tccctgttcg cgctctcatt cctttgatga
     8221 cactgtattc ggtgggatgc gggctgccat gagcagggtg ctctatttga atgatcaaga
     8281 ttgaccaaaa gccgcgctgc gcgggagtcg ccgtccaggg ttcggataga atgtccggtg
     8341 atgagtttcc cggacagcca gcccgaggct tcggcccatt cttcggccca ttcgttcgtg
     8401 accgacgggg tacgcgccgc cggcgcgctg gaggaggcgt gccggaggag tctggaggag
     8461 acgcaatctc tgcatctgga ggtagtgcgg gacctcgccg cggacctgag acgtcagctc
     8521 gacgctctgt cggggatggt agaggagcgt gcggagccgc gcgtggacgc ggcgctcgcc
     8581 tgcgcggacc ttgcgacact cgcggcttgc aacgtgatcc agatgcctcc cgcagaggcg
     8641 cgcacggtcg cgaatgccgt tcacatcgcc gccggagccg cccactctct gtcaggcacg
     8701 gcggaaaata tctcgaaaga cgaagcagag tacctggcgc gggacgcgcg cggtgcgcgg
     8761 tggaaggcgg acctcgcgtc ccggcaggcg gacgagttcc tgtcctcccg gagaagctag
     8821 aagggccgtc ccacgctctc tcccgcgagg agcgctaggg aagcctgcgc cacgcgccgc
     8881 aggtagtcgt cctcgtgcgg cacgaagttc gagaggctca cggtgatcgc gccggatccc
     8941 ccatcagcga actcccgggc gggagcggtg cgtccgggga gcagcctctc cgcgtcgccg
     9001 aggccgacgc ggacaacgga ggcgacctcc tcgcgctgca atcgcagatc accgggagga
     9061 gtggagtctt gcaggatata gacctcatgg aactcgcggt cgcgtccggc gggtatctcc
     9121 tgctcgacgc ggcgcgtacc cagcgggatg agccgggcgg ggtccacacg caggcccagt
     9181 tcctcctcaa gctctctcag ccctcccgcg accggctcct cgccggtccc gagatgtccg
     9241 gcggcggtga cgtctaggta tccgggccag gtgtccttga cggcggcgcg gcgctggact
     9301 agcaggtacg gtccatcgtc gtcagagccc gcgatccagc agtgaaaaca gcgatgccac
     9361 aggcccagac ggtgcgcctc gctcttccat gcgacctcgc cggtagggcg gccccactcg
     9421 tccaggatgt ctatcctctc gtccacggtg agagggtagc atcaaagccc gcgggactca
     9481 gccatcgccg ataaccgcta agctgaatgc gaagaaccgg gattgagatc caggaggtat
     9541 cacttgacca ccgtagtcat cgcgagctac ttcgaggagg agcacgtccg gcgcatccgg
     9601 gaggtggacg gacgggcgcg ggtactctac cgcgaggacc tcgtgccgcc gccgaggtgg
     9661 gacggcgacc atcggggcat cgacggctgg gaacggacgc cggaacagga cgaggagttc
     9721 ctggcgatgc tcgccgaggc tgaggttctg ctggacttcc cgcgcggaca cggccgcgag
     9781 ttgacgaagg tcgcgccgaa gctgcggtgg ttgcagggga gcatggccgg ggccggggag
     9841 ccggcccggc gggcgggttt gatcgagtcg gacatcgtag ttacgacggc gggcggggtc
     9901 tactccgggc cgctggccga gttcgtgctg ctggcgctcc tctcgcacgt aaaggacttc
     9961 gaccggctgc gctccgacca gcgggagcgg cgctggggcg agaggtacgt cgagacgctt
    10021 gaaggcaaga cgctttgtat cgtgggagtg ggcagcatcg gccgggcgat agcgcgccgg
    10081 gcgcggccct tcgggatgcg agtcctgggc gtcaagcgga cggtgcgcga ggaagatcca
    10141 gcccgcgagg aaaccgacga actgtacgag accgcccggc tgcgtgaagc cctcggcgag
    10201 gcggactacg tggcggcaac tctgccgctc acgccgcaga ccgagcggct gctggacgcc
    10261 gggaccatag cggcgatgaa gcccggcgcg tacttcgtga acgtcggtcg ggggaaggtg
    10321 gtggacgagg cggcgctcgt ggaggccctg cggagcgggc acctgtcggg ggcggctctg
    10381 gatgtgttcg aggaggagcc gctgccggga gagaacccat tgtggggtat gcagaacgtc
    10441 atcgtcagcc cgcactcgac ggacaacgtg ccgggcctga caaaccggct gctcacggat
    10501 ctcttctgcg agaacctgcg gcggtatctg gatggagaac cgctcaagaa cgagttgaac
    10561 aagcggctgc tgtattgaga cgctcccggg cggggcggat ccgtttgcaa gagtatgcgg
    10621 gatggtatat tttcagccct atctacatgg accttagcga gacatcgagt acctgtgcgc
    10681 ctcccggcgg gaggcgcgtc cgtaaattcc ccttcgagga gtcgttctgg aagctgtgat
    10741 cttccccgcg ctgcggatac ttggcgcgct ctctcttgtc gtgttgaacg ggttgttcgt
    10801 ggcggcggag ttctctctcg taaagatacg ggccactcag gtggaccggc tcgtggagga
    10861 aggccggact tcggccggga tagtcaagga ggccacgggc aagctcgacg cctacctcgc
    10921 ggtgtgccag ttggggatca cgatctcctc gctggccctc ggcgctctag gggagccggc
    10981 gatagcctcc ctcatagaac cggtgctgga gccgctcggt atagccggag gcacgctgca
    11041 cctcatcgcc tttatcatcg ggttcgggat catcacgttc ctgcacgtgg tcttcgggga
    11101 actcgcgccg aagagcatag ccatccagag cgccgagggg acctccctct tcgtcgcgcc
    11161 gttcatgaag ttcttctact accttctgct gcccgcgacc acggtcttca acgggacggc
    11221 caacctcttc atcaggatgc tcggctacgg ctccgcctcc gagagcgacg agacgcattc
    11281 ggagggtgag atccggaccc tcctcaccca gtcggcccag cagggagagg tggaagagga
    11341 cgaggaggac atgatcagcg cggtcttcga gctgaacgac aagtcggcgc gggagatcat
    11401 ggtcccaaga cccgacaccg tctctttgcc ggcagacctt cctcttcgca agctggtctc
    11461 ggtcgccgct gccggcaact acacccgcta ccccatctac gaggacgact cccccgacag
    11521 gatcatcggc atggtccacg taaaagacgt gctgcgctcg gttgacgaaa agggtctaga
    11581 agcggagata accgcccgcg acctcatgcg cggcgtgctc atagtcccgg agaaccgtcc
    11641 gatagaccag atcctggagg acttccagga ccaggagatc cagatggcca tagtcataga
    11701 tgagtggggc tcgttcgagg gcctcttcac catcgaggac atcctggagg agatagtcgg
    11761 cgagatccgg gacgagttcg acgaggagga acctgccgtc cgcaagctct ccgacgggtc
    11821 ctactctgta gacggccgca tccccatcag cgtcgtgaac gaggccctcg gctcggagtt
    11881 cgagagcgag gacttcgaca ccatcggcgg cctggtgctc ggcagcctcg gccgcgtccc
    11941 cgataccggc gacgaggtca cgctggacgg ctacgccctg cgcgtcgatg aggtggacgg
    12001 gacccgcgtc gcccaggtca taatccggga agcgcccccg gaagagagcg gcgagaccgg
    12061 agagtaaccg ggcgagcgct accgcccggc ggggatcagg acctcggaag acttcacgtc
    12121 cagcaagccc agcggcgtca gacgcagcgc gggcaggaag tcgcaggtca ggaagaggag
    12181 cgtgtagagg atgtcgtggt gctcgtagcc agcggcggac acagtttcgg agagccgccg
    12241 gttccgttca acgacttcct cgaacggtag atcggccatc atgccggtga tggggagggc
    12301 gagttcatag acgatttctc tgccctctac gattactatt cctccgttca gatccctgcc
    12361 ccgccgggcc gcgagagcca tagcctccgg gcggcgtccg agcaccagca gttgggttgt
    12421 ggtgttgtag gtgctggcga ggccatccag gtttgcggcg aacccggaga cgagggcgcg
    12481 ggagacccag gctccctccc ggtccacgag cgcggcgtgc agcaggtcgt cgtgaccatc
    12541 cagcgtgacg aggccgttct cggccgcgac ccgcgtatcg cggcgctcgg agatcacggc
    12601 ggacttcagg tgcattgccg gcaccctcgc ctccgtcccg gacgctctga ccgggtagag
    12661 tgcgggatcg gcgagatcca ggccgctgtc aaagcgcggg cggctccggt atttctccca
    12721 gtcgagac
//
LOCUS       DASSMB010000011         6796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_762245, whole genome shotgun sequence.
ACCESSION   DASSMB010000011 DASSMB010000000
VERSION     DASSMB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762245"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFJ72_00920"
     CDS             complement(<1..203)
                     /locus_tag="VFJ72_00920"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478054.1"
                     /translation="MCGICGEISTNGAADIAAVARMNETMNDRGPDGGGFWSQGGVAL
                     GHRRLKIIDLSECAAQPMTDPELG"
     gene            complement(205..1545)
                     /locus_tag="VFJ72_00925"
     CDS             complement(205..1545)
                     /locus_tag="VFJ72_00925"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET7478055.1"
                     /translation="MNEVYKPDGEARGFYRPLLEVLTNMGPEDFAERRERARVLLEDL
                     GATFPLPGEDNDRVLPADWMPRIIPRDHWEKLSAGLLQRGKAINAWILDLYNGSQSVV
                     PDEVIESSVFYRRDQLPASAVPVHVYGPDVVHLQSGEYVVLEDNVRVPSGVAYSEAVR
                     KAARAAFENTFEPYRVSRISPYYEMLRTTLGSAAPEDVEDPTIAIVTSGPGDSAYFEH
                     RRIAERCGIRLLTLDECCIEGGEVRMRSDGTRIDVIYRRVEGGYVVADLPELERVHRE
                     GRVGFANALGVGVADDKAVFAYVPAMIEHYLGEKPILNNAPTISLADPEQRAETLDRL
                     PELVLKPREGYGGLGVIIGPEADRETLDEARRNVEKNPEGFVAQECLDFSMHVLDDGD
                     GGQPGASFVDLRAFVLPAVDYVMPGGLTRVAKPGTRVVNSSSGGSFKDTWVLED"
     gene            complement(1556..2875)
                     /locus_tag="VFJ72_00930"
     CDS             complement(1556..2875)
                     /locus_tag="VFJ72_00930"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET7478056.1"
                     /translation="MTTFEGRCIGLEQEFFLVDATGTLSDRADEFLARCAELTRSAGR
                     DPEGFFPECARCMVEINTPPSYSLANLSREYLQNVELAMEAARDLGLRLYPLATYPLK
                     AEGSLRDEPRYELQARTVGRRRFENAGQCAGVHLHLETAAGAVDPRVGVSYESTRAAR
                     RGLLDLYNLATALDAALIALSRSCPFYADAATGLAMRTACYRGDPDLFPGGLYAELPL
                     VGGLLPYAESIEDLVEQQFARYYSWLGAMDRADVDPRLFERLGGGLLNSSSWNPVRLN
                     THGTVELRGLDSNYPETVLALLALVKTVADRVRRDGLRVVPDDDLRVFEISCGKLHVP
                     SFEYLSGDLFREAVSRGVESPEISAYLDSIFEFARDGEDYAALRSGGGYRTTEADILD
                     RYPGDTVLTRERGLALVLEACDELEAQVTRLKGWETPDMTAAGANGD"
     gene            3156..3404
                     /locus_tag="VFJ72_00935"
     CDS             3156..3404
                     /locus_tag="VFJ72_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478057.1"
                     /translation="MQTPRQSYSGRSLYVERDLIPIPEDLPAIGVAAGDEGLITALDL
                     RNNEVVALVQVSYSTGQPRGLVEMNVKPEERVLSYTAA"
     gene            complement(3432..4334)
                     /locus_tag="VFJ72_00940"
     CDS             complement(3432..4334)
                     /locus_tag="VFJ72_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7478058.1"
                     /translation="MGLAAIGLLALAASGAAAATSDGLEAAVTNAMLQEGDSARGLSV
                     SEKQERTQTNVERTSEDNKWAFGTAVIEAPKKEGYYPEGWLFVAQKSGGTWKVGLEGT
                     PEFSTLAEDAPDSVVDDGEKEMFSRSGGLELQSTSTGLQLPWKRGVRWKMSGGPHGWS
                     TGYDRPYSALDLTGGSGKVRAAGRGRVYTMCSTNRGWIRVYHPNGYSTDYYHLRKNIK
                     PRDGRRIRAGAFLGYTGTDVSCGGSASGRHVHFALLRGDRHVALDNKTIGGWTFQQGA
                     AYKGYAKHKTIKRFPPNGLLRNYG"
     gene            4703..5713
                     /gene="corA"
                     /locus_tag="VFJ72_00945"
     CDS             4703..5713
                     /gene="corA"
                     /locus_tag="VFJ72_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET7478059.1"
                     /translation="MIVDNAIYVDGHRTAEPHTLHETYQACREQHGVAWIGLYKPTEE
                     EFSSVTEEFELHPLAVEDAIKAHQRPKIERYDDTLFMVLRPARYVDETETVEFGEVHV
                     FVGEEFVVTVRHGEAPDLKRVRRRMEEEPDLLRRGSEAILHAILDRVVDDYGPVVEGL
                     ENDIDEIETEVFGGSPNVSRRIYELSREVIEFQRATRPLAGILEGLIKEDESDMDPEV
                     RRYLRDVQDHALRVEEQVAGFRELLQNILSVNLTLVGLSQNEEVKALSEASIEQNNEV
                     KKISSWAAILFAPTLIGTVYGMNFRHMPELSWLLGYPFALVLMAMVSCTLYLVFKRRG
                     WL"
     gene            5778..6773
                     /locus_tag="VFJ72_00950"
     CDS             5778..6773
                     /locus_tag="VFJ72_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7478060.1"
                     /translation="MPRRYILSIDGGGIRGIIPAIALTKLESVTGRPARETFSFVAGT
                     STGAIIAAALAAGIPASRILDIYLERAREVFPGYRPVNRLRQVFTGSMYSTRKIRDLI
                     SSEISAARGWTLNDSPIDILISAKRVPDGKPWYFVRDNPRNSGCTGRLGLVDCVTASA
                     AAPTYFQPWTVDDSKVPARCDRIGTLVDGSLGVAGNPVYQACVEAFHYSEGYAPEDAT
                     IVSLGTGRYLADRQPAWILPWLQWVLDELISSPGEQQTELVWRHFPQTAFYRIDTRLE
                     EDIRLDHIQSIDRLQEYGERLAELIDWKAIIAGTDEKFRVGRDQKTFPQYAQPTT"
BASE COUNT         1192 a         2259 c         2129 g         1216 t
ORIGIN      
        1 ccgagctccg ggtcggtcat cggctgggcg gcgcactcgg agaggtctat gatcttcaac
       61 cgccggtggc cgagtgccac gccgccctgg ctccagaacc cgccgccgtc cgggccgcgg
      121 tcgttcatcg tctcgttcat ccgggcgacg gccgcgatat ccgccgcgcc gttcgtactg
      181 atctcgccgc agatcccgca catatcagtc ctccagtacc caggtatctt tgaagctccc
      241 cccggaagag gagtttacga cgcgcgtacc cggcttcgcc acccttgtga ggccgccggg
      301 catgacgtag tccactgccg ggagcacgaa cgcccgcagg tccacgaacg acgcgcccgg
      361 ctggccaccg tcgccatcat ccaggacgtg catcgagaag tccagacact cctgcgccac
      421 gaaaccctcc gggttcttct ccacattgcg ccgggcctcg tccagcgtct cccggtcggc
      481 ctccgggccg atgatgacgc cgaggccgcc gtaaccctcg cgcggtttca ggaccagttc
      541 cggcagccga tccagagtct cggcgcgctg ctctggatcg gcgagcgaga tcgtcggggc
      601 attgttcagg atgggcttct caccgaggta gtgctcgatc atggccggca cgtaggcgaa
      661 gacggccttg tcgtccgcca ccccgacccc gagggcgttg gcgaagccga cccggccttc
      721 gcggtggacg cgctccagct ccgggaggtc tgcgaccacg taaccgcctt ctacgcggcg
      781 gtagatcacg tctatccgcg tcccgtccga ccgcatccgc acctctccgc cctcgatgca
      841 acattcgtcc agcgtcaaga gccggatgcc gcaccgctcg gcgatgcggc ggtgctcgaa
      901 gtacgccgag tctccggggc cgctggtcac gatggcgatg gtcgggtcct cgacatcctc
      961 cggcgcggcg gagcccagcg tggtgcgcag catctcgtag tagggtgaga tgcggctgac
     1021 ccggtacggc tcgaacgtat tttcgaaggc cgcgcgggcg gcctttcgga cggcctccga
     1081 gtaggcgacg ccgctcggga cgcgcacgtt gtcctccagc acgacgtact cgccgctctg
     1141 gaggtggacc acgtccgggc cgtagacgtg tacggggacg gcggaggcgg ggagctggtc
     1201 ccggcggtag aagacgctgc tctctattac ctcgtcgggg actacggact gggagccgtt
     1261 gtagaggtcc aggatccagg cgttaatcgc cttcccgcgt tgcaggagtc cggcggagag
     1321 cttctcccag tgatctcggg gtatgatcct gggcatccag tccgccggga ggacccggtc
     1381 attgtcttct ccgggcagcg ggaaggtcgc gccgaggtct tcgagcagga ccctcgcccg
     1441 ttcgcgccgt tcggcgaagt cctccggtcc catgtttgtc aggacctcca gcagcggccg
     1501 gtagaagccg cgagcctcgc cgtccggttt gtagacttcg ttcatgcgcg ccgattcaat
     1561 caccgtttgc gccagccgcc gtcatgtccg gtgtctccca gcccttgagc cgagttacct
     1621 gcgcttccaa ctcgtcgcag gcttcgagga cgagtgcgag cccacgctcc ctcgtgagga
     1681 cggtatctcc ggggtagcga tccaggatgt cggcctccgt ggtgcggtag ccaccgccgg
     1741 acctgagcgc cgcgtagtcc tctccgtctc ttgcgaactc gaagatggag tctagatacg
     1801 cggagatctc tgggctttcg acgcctcttg agacggcttc ccggaagagg tctccgctca
     1861 ggtactcgaa gctcggcacg tggagcttgc cgcaggagat ctcgaagacg cgcaggtcat
     1921 cgtccgggac gactcgcagg ccatcccggc gcacccggtc cgcgacggtc ttgactagcg
     1981 cgagcagtgc cagcacggtc tccgggtagt tgctgtccag gccgcgcagc tcgaccgtgc
     2041 cgtgcgtgtt gagacgcacc gggttccagg aggacgagtt cagaaggccg ccgccgagac
     2101 gctcgaagag cctggggtcc acgtccgccc ggtccatcgc tccaagccag gagtagtagc
     2161 gggcgaactg ctgctcgacc aggtcctcga tgctctccgc gtatggcagc aggccgccga
     2221 cgagcggtag ctccgcgtag aggccgccgg ggaagaggtc cgggtcgccc cggtagcagg
     2281 cggtgcgcat cgccagtcct gtcgccgcgt ccgcataaaa ggggcacgag cggctgaggg
     2341 cgatgagcgc ggcgtccagc gcggttgcaa ggttgtagag atccagaagc ccccttcgcg
     2401 ccgctcgcgt agattcgtag gagacgccca ccctggggtc aacggctcca gccgcggtct
     2461 ccaggtgcag gtgcactccg gcgcactgcc ccgcgttctc gaagcgccgc cgcccgacgg
     2521 tccgcgcctg aagctcgtag cggggctcgt cgcgtagtga gccctccgcc ttcagagggt
     2581 aggtcgccag aggatagagc cggaggccga ggtcgcgggc cgcctccatc gccagctcga
     2641 cgttttgcag gtactcgcgg gagaggttcg cgagggaata agatggaggg gtgttgatct
     2701 cgaccatgca ccgggcgcac tccgggaaga aaccttccgg gtcgcgtccc gccgagcgcg
     2761 tcagctccgc gcagcgtgcc agaaattcgt cggcccggtc agagagagta cccgtagcgt
     2821 ccaccaggaa gaattcctgc tccagtccta tgcaacgtcc ctcgaaagtg gtcaaaacac
     2881 ctccatctcc gggctcgacc gcagccaggt aaatctaccg ccccttcagg gcattgtaac
     2941 tggcccaatg gataacttcc cgcttcaagc gggcctggat ctctggtcag cgggaagagg
     3001 ctggtagagg atctaagcgc gaccccctgc tgtccggcca acgaaggacc gccgcgagcg
     3061 ggagattctt gtgcggcggg ccgaagccgg cggcgggcgc tctaccgtag gatgcgactg
     3121 tcgttagaat catggaccga gagcgaagga gagacatgca gacgcccagg caaagctaca
     3181 gcggacgctc gctatacgtg gagcgggacc tgatccccat ccccgaagac ctgcccgcta
     3241 tcggagtcgc ggctggggac gagggcctga ttaccgccct cgaccttcgg aataacgagg
     3301 tcgtcgctct ggtacaggtc tcgtactcga ccggccagcc tcgcggcttg gtcgagatga
     3361 acgtgaagcc cgaggagaga gtcctctctt acaccgccgc ctgagcgagt cctacgcggg
     3421 cagatgggct tttaaccgta gtttctgagt aagccgttcg gcgggaagcg ctttatggtc
     3481 ttatgcttag cgtagccctt gtaggccgcg ccctgttgga aggtccagcc gccgatagtt
     3541 ttattgtcca gggcgacgtg ccggtcgccc cgcagcaggg caaagtggac gtgcctgccg
     3601 gacgcggagc ctccgcagga gacgtccgtg ccggtatagc cgaggaacgc gcccgccctg
     3661 atccgcctgc cgtcgcgcgg ctttatgttc ttcctgagat gataatagtc ggtagagtag
     3721 ccgttcgggt ggtagacccg gatccaccct cgattagtgc tgcacatggt gtacacgcgg
     3781 ccgcgtcccg ccgcccggac tttgccgctg ccgccggtca ggtcgagggc gctgtatggc
     3841 cggtcatagc cggtcgacca gccgtgcgga ccgccggaca tcttccaccg cacgccgcgc
     3901 ttccacggca gctgcagccc cgtactcgtg gattgcaact ccaggccgcc gctccgggag
     3961 aacatctctt tctcgccgtc gtctaccacg gagtctggag cgtcttcggc gagcgtcgag
     4021 aactccgggg tcccttccag acctaccttc caggtgccgc cggacttctg cgccacgaag
     4081 agccagccct ccggatagta gccttctttc tttggagcct cgatcaccgc cgtcccgaaa
     4141 gcccacttgt tgtcctcact cgtgcgctcg acgttcgtct gcgtccgctc ctgtttctcg
     4201 ctcacggaga gccctcgcgc cgagtctccc tcctgaagca tcgcgttcgt caccgcggcc
     4261 tccagcccat ccgaagtcgc cgctgccgcc ccggacgccg ccagcgccag cagaccgata
     4321 gccgcgagac ccatcactgt gagcgggcga attatcccgc tcctgtcttc tccaaactct
     4381 ctactattgc tattcatcgc gatatacccc ccctgtcttc gcgatacatg gaaaatctat
     4441 ctgctccggc gcgagcttga ctgtatctgg ccggcaagtg tcgtacttta tacgtcgtga
     4501 atccgggtga ggatcaaacc tggcttcacg aaaacagagt agccccctcc tctgaactca
     4561 gttatttgct cccggcgccg gtggcgctgg aagaccgcgt acatttactt acaaccggcc
     4621 ggtaatgtca agatctctcc agggctcgaa ccgccagcgg atggttctca ggggaagcgc
     4681 cgcatgagcg tataatgacg ccatgatcgt ggacaacgcc atctacgtgg acggccacag
     4741 gacggccgag ccccacacgc tgcacgagac ttaccaggcc tgccgggagc agcacggggt
     4801 ggcctggatc ggcctctaca aaccgaccga ggaggagttc tcgtcggtaa cggaggagtt
     4861 cgaactgcac ccgctggcgg tcgaggacgc gatcaaggcg catcagcgcc cgaagatcga
     4921 acgctacgac gacaccctct tcatggtcct gcgcccggcc cgctacgtgg acgagaccga
     4981 gacggtcgag ttcggcgagg tccacgtctt cgtgggggag gagttcgtgg tgacggtccg
     5041 tcacggcgag gccccggacc tcaagcgcgt gaggcgcagg atggaggagg agccggacct
     5101 cctgaggcgc ggttccgagg cgatcctgca cgccatcctg gaccgtgtgg tggacgacta
     5161 cggtcccgtg gtcgagggac tggagaacga catagatgag atagagaccg aggtattcgg
     5221 cggcagtccc aacgtctcgc ggcgcatcta cgagctctcg cgcgaggtta tcgagttcca
     5281 gcgggccact agacccctgg ccggtatcct ggagggactg atcaaggagg atgaaagcga
     5341 catggacccg gaggtcagga ggtacctgcg cgacgtgcag gaccacgccc tgcgggtcga
     5401 ggagcaggta gcgggtttcc gcgagctgtt gcagaacatc ctgagcgtca acctcaccct
     5461 ggtcggcctc tcccagaacg aggaggtgaa ggcgctctcc gaggccagca tcgaacagaa
     5521 caacgaggtc aagaagatct cctcctgggc cgccatcctc ttcgctccca cgctgatagg
     5581 gaccgtctac ggcatgaact tccgtcatat gccggaactg agctggctcc tcggttaccc
     5641 gttcgccctg gtgctgatgg cgatggtctc ctgcactctc tacctggtct tcaaacgccg
     5701 cgggtggctg taactctctg atccctatcg gcctcatact ggtatgcgga gttatcccgg
     5761 aatagagagg aagaagcgtg ccccggcgat acattttatc aatcgacggc ggcggcattc
     5821 gcggcatcat cccggcgatc gccctgacga agctggagag tgtaacggga cgcccggctc
     5881 gcgagacgtt ctcgttcgtc gccggcacct ccaccggagc tatcatcgcg gcagcgctcg
     5941 ccgccggaat ccccgcctct cgcatcctgg acatatacct cgaacgcgcc cgcgaggtct
     6001 tccccggata ccgtcccgta aacaggctga ggcaggtctt caccggctcc atgtactcga
     6061 cgcggaagat acgcgacctg atctcttcgg agataagcgc cgcgcggggc tggacgctca
     6121 acgactcgcc gatcgacatc ctcatctctg ccaagcgcgt cccggacggc aaaccctggt
     6181 acttcgtgcg cgacaacccg agaaactccg gctgcaccgg gcgtctcgga ctggtcgatt
     6241 gtgtcaccgc ctccgccgct gcgccgacct acttccaacc ttggaccgtg gacgattcga
     6301 aggtcccggc ccgctgcgat cgcatcggaa ccctggttga cggcagcttg ggcgtggcgg
     6361 gcaaccccgt ctaccaggcg tgcgtcgaag ccttccacta ctccgagggt tacgcgccgg
     6421 aagatgcgac tatcgtctct cttggaacgg ggcgctacct cgccgaccgg caacccgcct
     6481 ggatcctgcc gtggctccag tgggtgctgg acgagctgat ctcatccccc ggagaacaac
     6541 agaccgaact cgtctggcgc cacttccccc agacggcctt ctaccgcatc gacaccaggc
     6601 tcgaagaaga catcaggctc gaccacattc agagcatcga ccgcctgcaa gaatacggcg
     6661 agcggttggc cgaactcatc gactggaaag ccatcattgc gggcacggac gaaaagttcc
     6721 gcgtcggccg cgaccagaag acctttcccc aatacgcaca accaacgacg tagagactca
     6781 tcgaacgtgc gtgatc
//
LOCUS       DASSMB010000012         5871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_382432, whole genome shotgun sequence.
ACCESSION   DASSMB010000012 DASSMB010000000
VERSION     DASSMB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382432"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /gene="tgt"
                     /locus_tag="VFJ72_00955"
     CDS             complement(<1..1113)
                     /gene="tgt"
                     /locus_tag="VFJ72_00955"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7478061.1"
                     /translation="MEVEGRDGEARAGVLHTPHGAVETPVFMPVGTKGTVKGLTPGDL
                     RTAGAGVVLGNTYHLYLRPGADLVREAGGLHGFTGWDGPMLTDSGGYQVFSLAKTRRI
                     SEEGVAFSSVYDGSKHTFTPELTTQVQEDLGADIAMVLDECPPARADREYHERSLRRT
                     ARWAQRCKAAHTREDQALFGIVQGGLHADLRHESLERTVEVDFPGYAVGGLSVGESRE
                     EMFETLSLVTPRLPVERPRYFMGIGDPVGILGVISLGVDMFDCVLPTRLARHGAALTP
                     DGRMNLKNARYRRDFGPLDPECDCETCAGYSRAYLAHLVRENELLGHRLITLHNVHFT
                     LRLCRTARGRIVAGDFEKWAAAWTRRYEAGGLLREGK"
     gene            complement(1128..2126)
                     /gene="queA"
                     /locus_tag="VFJ72_00960"
     CDS             complement(1128..2126)
                     /gene="queA"
                     /locus_tag="VFJ72_00960"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET7478062.1"
                     /translation="MLTSELDYALPEALIAQKPAEPRDSSRLMVVDARTGDISHHVFR
                     DLPGFLRSGDALVLNETKVIPARLEARRPGGGAVELLFLRDLGGRWEVLARPSKRLRP
                     GMKLLAGDEELDVVKPLGEGHWTVAAEDVPALLERHGHMPLPPYIRATPEAEARYQTV
                     YARNAGSAAAPTAGFHFTPEVLEEAAKAGAEVARVTLHVGTGTFAPVRAERLEEHRMH
                     AEHYAVPEEAERIVREADRVVAAGTTVARTLETWAASGEREGESRLFVYPGYRWRAVD
                     ALLTNFHLPRSTLLAMVMSFGGTDLVREAYRVAVEDRYRFYSFGDAMLILNGGRSL"
     gene            complement(2136..2354)
                     /locus_tag="VFJ72_00965"
     CDS             complement(2136..2354)
                     /locus_tag="VFJ72_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET7478063.1"
                     /translation="MGLEGIGKLLIGGAIVLLALGGLFLLLGRFGLDRLPGDLVFRRG
                     GLTVYFPIGLMILLSVVGTIVLNLIFRR"
     gene            2652..4526
                     /locus_tag="VFJ72_00970"
     CDS             2652..4526
                     /locus_tag="VFJ72_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HET7478064.1"
                     /translation="MVRRFWLLGALLLALSALVGGAAFAHDDFTSDGGIREGSVERHL
                     YQHGGDAGHLPSSAKNVDLISKLQLKNVEPEKIADVGVSPSGDYAFLAAWGGATCKYN
                     GVHVVDIANPAQPKEVSFIQSKEGSYPGEGVQALNLTTSAFNGDILVTNNEICKDKAG
                     FGGMNIYNVTDPRHPTPLVEGIGDSTVNGQGKKGANEIHSVYAWDAGTKAYAVIVDNE
                     EAMDVDILDITNPKNAKLIAEYDLAQKFPQILQSAPENLTEVFLHDMVVKKIGTRQVM
                     LASYWDGGYVKLDVTDPLAPTYLGDTDFTNPDPEAAESGLTVPPEGNGHEAEFTLDNR
                     FVVGADEDFAPFALVARNVDDNTVIHASQGSNTRKLEEGQTITGTSVFAGRACPGDPA
                     VPAGDPNATDVAVVERGVCTFTEKVQSVLAAGGYDAVLIFNRVGSDACETPLSMSVEG
                     DIPTFGVAPRSQGYAIFDIEGSYDEAACLAAPAGSQLAPIAVGAKGDTLTFSSYFDGW
                     GYVHLFGNTGGKMAELDTYAIPEAHDPAYANGFGDLSVHEVATSKVNASRMYFSYYSG
                     GFRVARITADQKIAEVGHYIAPGGNNLWGVEVFQDAGQEYVAASDRDSGLFIFKYTGP
                     "
     gene            complement(4602..5111)
                     /locus_tag="VFJ72_00975"
     CDS             complement(4602..5111)
                     /locus_tag="VFJ72_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7478065.1"
                     /translation="MVLLETRKQAHHVSSQTRIGHVHLKVADIERALRFYRDILGFEV
                     MQWYGEDAVFLSAGGYHHHIGLNTWMSKNAPPAPRNSAGLFHLAILYPDRRELAQALR
                     WLMDAEYPLDGASDHGVSEALYLRDPDGNGVELYADRPQERWPRTPDGGLAMVTEPLD
                     IEGLLSALD"
     gene            5395..>5871
                     /locus_tag="VFJ72_00980"
     CDS             5395..>5871
                     /locus_tag="VFJ72_00980"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7478066.1"
                     /translation="METIDTRRHSRVILEGRDRAPARSYLKGMGYSEEDLHKPIVGVA
                     HSWIETMPCNFNHRHLAEKVKEGIREAGGTPMELNTISISDGITMGTQGMKTSLVSRE
                     VIADSIELVGRGQMFDAVVTIVGCDKTIPAAAMAHVRLGIPGVIVYSGSIKPGRFRG"
BASE COUNT         1030 a         1980 c         1971 g          890 t
ORIGIN      
        1 tttgccttcc cgcagtaacc ctcctgcctc gtagcgccgg gtccaggcgg cggcccactt
       61 ctcgaagtct cccgccacga tgcgcccccg cgccgtccgg cacaggcgca gggtgaagtg
      121 gacgttgtgc agcgtgatga gccggtgccc gagaagctcg ttctcccgca cgagatgagc
      181 cagataagcc cggctgtagc ccgcgcaggt ctcgcagtca cactccgggt ccagcggccc
      241 gaagtcccgc cggtagcggg cgttcttgag gttcatgcgg ccgtccgggg tgagggcggc
      301 gccgtggcgg gcgaggcgcg tgggcaggac gcagtcgaac atgtccacgc cgagcgagat
      361 cacaccgagt atccccaccg ggtccccgat ccccatgaag taccggggtc tctccaccgg
      421 gagccgcggc gtaacgagag agagcgtctc gaacatctcc tcccgagact cgcccaccga
      481 gagcccgccg acggcgtagc cgggaaaatc cacctcgact gtccgctcca ggctttcgtg
      541 gcgcaggtcc gcgtggagtc cgccctgcac gatcccgaac agcgcctggt cctccctcgt
      601 gtgcgccgcc ttgcaccgct gggcccaccg ggccgtccgt ctgagcgacc gctcatggta
      661 ctcccggtcc gcccgagccg gcgggcactc gtccagcacc atcgcaatgt ccgcccccag
      721 gtcctcctgc acctgcgtcg tcagctccgg cgtgaaagtg tgcttcgagc cgtcgtagac
      781 cgaggaaaac gcgacgccct cctcggagat ccggcgcgtc ttcgccagcg agaacacctg
      841 atacccgccc gaatcggtca gcatcggccc gtcccagccg gtgaacccgt gcagcccgcc
      901 cgcctcgcgc acgagatccg cgcccggccg caggtacaga tggtacgtgt taccgagcac
      961 caccccggcc cccgccgtcc gcaggtcgcc cggcgtgagc cccttgacgg tccccttcgt
     1021 cccgaccggc atgaagaccg gcgtctcgac cgcgccgtgc ggagtgtgga gaaccccggc
     1081 ccgcgcctcg ccgtcgcgac cctcgacctc tatagtgacc ggcgcgccta caggctcctc
     1141 ccgccgttga ggatgagcat cgcgtccccg aaggagtaga acctgtagcg gtcctccacc
     1201 gcgacccggt acgcctcgcg cacgaggtcc gtcccgccga agctcattac catcgccagc
     1261 agcgtcgagc gcggcagatg gaagttggtg agcagcgcgt caaccgcccg ccacctgtag
     1321 ccgggataca cgaagagccg cgactctccc tcccgctccc cgctcgccgc ccaggtctcc
     1381 agggtccggg cgacggtcgt cccggcggcg acgacccgat ccgcctcccg gacgatgcgc
     1441 tccgcctcct ccggcacggc gtagtgctcg gcgtgcatcc ggtgttcctc cagacgctcg
     1501 gcccggaccg gagcgaaggt cccggtcccg acgtgcagcg taacccgcgc cacttcggct
     1561 cccgccttag ccgcctcctc cagtacctcc ggggtgaagt ggaagcccgc ggtcggggcg
     1621 gcggcggagc cggcgtttct cgcgtacacg gtctggtatc gggcctcggc ttccggcgtc
     1681 gcccggatgt acggcgggag cggcatgtgt ccgtgacgct caagaagggc cggcacgtcc
     1741 tccgcggcga cggtccagtg accctcgcca agcggcttca cgacgtctag ctcctcgtcg
     1801 cccgcgagga gcttcatccc cggccgcagg cgcttgctcg ggcgggcgag gacctcccag
     1861 cgccctccga ggtcccgcag gaagagaagc tccaccgcgc cgcctccggg acggcgggct
     1921 tcgagccggg cggggatcac cttcgtctcg ttcaagacca atgcgtcgcc ggagcgcagg
     1981 aagccgggca ggtcgcggaa gacgtggtgg gagatgtcgc cggtccttgc gtccactacc
     2041 atcaggcgcg aggagtcgcg gggttcggcg ggtttctggg cgatcagggc ttcgggcagg
     2101 gcgtagtcga gctcggaggt aagcaagaac tgtccctagc ggcggaagat caggttcagg
     2161 acgatggtcc cgacgacgga gagcaggatc atgagcccta tcgggaagta gacggtcagc
     2221 ccgccgcgcc tgaagaccag gtcacccggc agccggtcga gaccgaaccg cccgagcaac
     2281 aagaacagcc cgccgagcgc gagcagcacg atcgcgccgc cgatcagcaa ctttcctatg
     2341 ccttcgagtc ccatgtggat attctatgcc gcgcaccgcc gatatccagt cgagctagcg
     2401 cacgctcttg tgcggcatgt agcgctattg tatgctgccg agcatcccgc aggcgcgtag
     2461 tgaggtccgg taggaaacct tcaggcatgg tctgggcgat tggcaggtgt tgagcaggaa
     2521 ggggtgatgc ggtcgagaag gcaatcttac ctattggaat gagtgctggt gtggtggtcg
     2581 gcacgtcgag ccggggcttc cggggagaac cgtcgaatgg ttaagcaagg ttgagtagac
     2641 aggggagagc attggttaga aggttctggc tgctgggagc gttgctcctg gcgctatcgg
     2701 cgttggtggg cggtgcggcg ttcgcgcacg acgacttcac gtccgacggc gggatccggg
     2761 aggggagcgt cgagcggcac ctctaccagc acggcggcga cgcgggacac ctgccttcga
     2821 gcgcgaaaaa cgtcgacctc atcagcaagc tgcagctcaa gaacgtcgag ccggagaaga
     2881 tcgccgacgt cggcgtttcg cccagcggcg actacgcctt cctggcggcg tggggcggcg
     2941 cgacgtgcaa gtacaacggc gttcacgtgg tcgacatcgc caacccagcc cagcctaaag
     3001 aggtgtcctt catccagtcc aaggagggca gctaccccgg cgagggcgtc caggcgctca
     3061 acctgaccac cagcgcgttc aacggggaca tcctcgtcac caacaacgag atctgcaagg
     3121 acaaggccgg cttcggcggc atgaacatct acaacgtcac cgacccgcgg cacccgaccc
     3181 cgctggtgga gggcatcggc gactccaccg tgaacggcca gggcaagaag ggcgcgaacg
     3241 agatccacag cgtctacgcc tgggacgccg gaaccaaggc gtacgccgtg atagtggaca
     3301 acgaagaggc gatggacgta gacatcctcg acatcacgaa ccccaagaac gcgaagctaa
     3361 tcgccgagta cgacctcgcg caaaagttcc cgcagatcct gcagtcggcg ccggagaacc
     3421 tcaccgaagt gttcctccac gacatggtgg tcaagaagat cggcacccgt caggtgatgc
     3481 tggcctccta ctgggacggc ggctacgtga agctcgacgt gaccgacccg ctggctccga
     3541 cgtacctggg cgacaccgac ttcacgaacc ccgaccccga ggcggccgag agcggtctca
     3601 ccgtgccgcc ggagggcaac ggccacgagg ccgagttcac cctcgacaac cgcttcgtcg
     3661 tcggcgcgga cgaggacttc gcgcccttcg cgctggtcgc gcgcaacgtg gacgacaaca
     3721 ccgttatcca cgccagccag ggcagcaaca cgcgcaagct cgaagaggga cagaccatca
     3781 ccggaacctc ggtgttcgcc ggtcgcgcct gccccggaga tcccgcggtg ccagcgggcg
     3841 acccgaacgc cacggacgtc gcggtcgtcg agcgcggcgt ctgcacgttc accgagaagg
     3901 tgcagagcgt gctggcggcc ggcggctacg acgccgtcct gatcttcaac cgcgtcggct
     3961 ccgacgcctg cgagacgccg ttgagcatga gcgtcgaggg cgacatcccg accttcggcg
     4021 tcgcgccccg ctcgcaaggc tacgccatct tcgatatcga ggggagctac gacgaggcgg
     4081 cttgcctggc cgcgccggcg ggttcgcagc tcgctcctat cgccgtcggc gcgaagggcg
     4141 acacgctgac gttctcgtcg tacttcgacg gttggggcta cgtgcacctg ttcggcaaca
     4201 ccggcggcaa gatggccgag ctcgacacct atgcgatccc ggaggcgcac gacccggcct
     4261 acgcgaacgg gttcggcgac ctgtcggtcc acgaggtagc cacctcgaag gtgaacgcga
     4321 gccggatgta cttctcgtac tactccggcg gcttccgggt cgccaggatc accgccgacc
     4381 agaagatagc ggaggtcggg cactacatcg cgcccggcgg caacaacctc tggggcgtcg
     4441 aggtcttcca ggacgcgggc caggagtacg tcgccgcgag cgatcgcgac agcggcctct
     4501 tcatcttcaa gtacaccggt ccgtaaacgc caccgacgca gcaaagaaga ggggggcgat
     4561 ccgaacggat cgcccccctc ttcgtccgct cctgctgccg gctaatccag tgcggagagc
     4621 aggccctcga tgtccagcgg ctcggtgacc attgcgaggc cgccgtcggg ggttcggggc
     4681 caccgctcct ggggccggtc ggcatagagt tccacgccgt tgccgtccgg gtcgcgcaga
     4741 tagagcgcct cggagacgcc gtggtcggag gcgccgtcca gggggtactc ggcgtccatg
     4801 agccagcgga gggcctgggc cagctcccgg cggtccggat agaggatggc gaggtggaag
     4861 aggcccgccg agttgcgcgg agcgggcgga gcgttcttgc tcatccaggt gttcaggccg
     4921 atgtggtggt ggtagccgcc cgcggagagg aagaccgcgt cctccccgta ccactgcatg
     4981 acctcgaatc cgaggatgtc gcggtagaag cggagggctc gttctatatc cgccaccttc
     5041 aggtggacgt gcccgatgcg ggtctgggag cttacgtgat gggcctgctt gcgggtctcc
     5101 aaaagaacca cctttctcgt cagtgatcct agattgtagc gtccgggccc gccagcggcc
     5161 gcgaggagcc gccgaagcgc cggacggccg ggcgcgtacg agactccggc ccggcgcgta
     5221 tcctagagaa ggcggaggcc aggatccggc cggcaacgga gaacgcggag tttacgagac
     5281 ggcttcacgg ctccagtccg ggggaacggg atccaagccc ttatggcgcg gctttcgata
     5341 ctttctatat actcaggaac tcaggagcgt cgtccgaaac tagtgggaga gagcatggag
     5401 accatcgata ctcgccgtca cagccgcgtc atcctggagg gccgcgaccg cgccccggcg
     5461 cgctcgtacc tcaaggggat gggctactcg gaggaggacc tgcacaagcc gatagtcgga
     5521 gtggctcact cctggatcga gacgatgccc tgcaacttca atcaccgcca cctggccgag
     5581 aaggtgaagg agggcatccg cgaggcgggc ggcaccccga tggagctgaa caccatctcc
     5641 atctccgacg gaataacgat ggggacgcag gggatgaaga cctcgctcgt gagccgcgag
     5701 gtgatcgcgg actccataga gctggtcgga cgcgggcaga tgttcgacgc cgtggtgacc
     5761 atcgtcggct gcgacaagac gataccggcg gcggcgatgg cgcacgtgcg gctcgggatt
     5821 cctggcgtca tcgtgtactc gggatcgatc aagccggggc ggttcagggg g
//
LOCUS       DASSMB010000013        13884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_2987, whole genome shotgun sequence.
ACCESSION   DASSMB010000013 DASSMB010000000
VERSION     DASSMB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13884
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2987"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..751
                     /locus_tag="VFJ72_00985"
     CDS             395..751
                     /locus_tag="VFJ72_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET7478067.1"
                     /translation="MAASGDAVVAEAQTWLGVPYVYGGSSRYGVDCSGLTQAVFGEFG
                     VPLPHSAASQFGYGVSVAAPAAGDLVFSDFTGGGVGHVGIATGDGSMINAPYPGTTVR
                     YDPISPANVVGYKSVL"
     gene            complement(858..1313)
                     /locus_tag="VFJ72_00990"
     CDS             complement(858..1313)
                     /locus_tag="VFJ72_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7478068.1"
                     /translation="MLSPFRGFMDVQGEFDRLFDEMMRNVPWRGSGQRSAKPATWAPA
                     IDVLTKDGDLLIRAELPGVKQEDVDITLSQGLLTISGKSGAEREEQHGGYYVRERRQG
                     SFRRSIALPEGTDESKIQARFEDGVLEVTVTGAAAIEEPKRIQIEGPGE"
     gene            1486..1977
                     /locus_tag="VFJ72_00995"
     CDS             1486..1977
                     /locus_tag="VFJ72_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7478069.1"
                     /translation="MSDGRVGPNLAAMRERRMWTQARLAREAGVSPTTVSGIEGGRIS
                     RPHFGTLRKLARALEVPAEELLGEISPGPLESGEQMSLEWARSAREEEFERRLDGATL
                     HSLNALSRELDEEHGRLRTLYGEFPKGSEQRRFIKRQIRDISGRSGSVGASIEFHDDW
                     EEP"
     gene            complement(2068..2433)
                     /locus_tag="VFJ72_01000"
     CDS             complement(2068..2433)
                     /locus_tag="VFJ72_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7478070.1"
                     /translation="MKRVLLVEDHSSFRRALAFVLEREPDLRVVAQAGSLAEARSRVN
                     GESEFDVAVLDLGLPDGNGMDLISELRKANPDASIVVLTVDLNPKNQDLALEKGADAV
                     LGKEASITQIVAAVRNLQA"
     gene            complement(2541..2768)
                     /locus_tag="VFJ72_01005"
     CDS             complement(2541..2768)
                     /locus_tag="VFJ72_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478071.1"
                     /translation="MKMRSGCFGLLMELGAWVLSTILTTVGVLMLLVGALTFGPSIEP
                     LGVLLIVAAPIGALVLIKVGRDIMSDLRRPK"
     gene            complement(2880..2952)
                     /locus_tag="VFJ72_01010"
     tRNA            complement(2880..2952)
                     /locus_tag="VFJ72_01010"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2917..2919),aa:Phe,seq:gaa)
     gene            complement(3041..3385)
                     /gene="trxA"
                     /locus_tag="VFJ72_01015"
     CDS             complement(3041..3385)
                     /gene="trxA"
                     /locus_tag="VFJ72_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7478072.1"
                     /translation="MAAPVDVTASNFEAEVLNSEIPVLVDFWAAWCGPCRQVAPVMEQ
                     IADEYDGSVKVAKVDVDAEQELAGAFQISSIPTIALFEPGEQPKAVMGALPKEMLESA
                     FDLNRFSGSKQR"
     gene            complement(3439..3660)
                     /locus_tag="VFJ72_01020"
     CDS             complement(3439..3660)
                     /locus_tag="VFJ72_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478073.1"
                     /translation="MREMVREMGQERVALRTRRTTVVLLQVASFVCLGVAFASLAGYY
                     VPHAVGPSILLMVTAVVGFALSIIEARGS"
     gene            complement(3823..3896)
                     /locus_tag="VFJ72_01025"
     tRNA            complement(3823..3896)
                     /locus_tag="VFJ72_01025"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3860..3862),aa:Asp,seq:gtc)
     gene            complement(4160..5626)
                     /locus_tag="VFJ72_01030"
     CDS             complement(4160..5626)
                     /locus_tag="VFJ72_01030"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET7478074.1"
                     /translation="MSANTAERTIVDKVEKRLFVGGEWRDAAGGGTLPVEDPSTGEAL
                     CEVADATPEDAMAALDAASEVQKEWAKHPPRERGEILRRAYKKLVERIDELALLMTLE
                     MGKPIGESKGEILYAADFLRWFSEEAVRIDGRYAVAPNGAGRILTMKQPVGPCLAITP
                     WNFPMAMGTRKIGPAIAAGCTIVVKPAQLTPLSMLALAQILEESGLPPGVLNVVTSSS
                     SGATMSPLIQDRRLRKLTFTGSTPVGRKLMEQASGNLLRLSMELGGNAPFLVFDDADL
                     DAAVEGAMLAKMRNIGEACTAANRFHVADSVADEFAEKLVEKMSALKVGRGTEEGVQV
                     GPLIDDDQRGKVSDLVDDALSKGAKALVGAGKMDGRGYFYEPTVLGDVPEDADLRQEE
                     IFGPVAPIFSVSSEEEAIEAANDTEYGLVAYVYTRDLKRALRVCEGLETGMVGLNRGM
                     VSNASAPFGGVKHSGFGREGGAEGIEEYLETKYISVDL"
     gene            5799..6587
                     /locus_tag="VFJ72_01035"
     CDS             5799..6587
                     /locus_tag="VFJ72_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET7478075.1"
                     /translation="MEARKPYYEVYAIRYATREARSGENFYGHDPHDAPMPMDYFVWA
                     AVSEEHTVVIDAGFNAEVAARRGRDHLRCPTDGLRELGIDSEEVPLVILTHLHYDHVG
                     NLEKFPAATFVVQEDEMRFWTGRYAGRPHFRETVEPDDVLHLVRENFAGRLRFVSGSE
                     EVVPGVEVHRTGGHSPGLQVVRVDTVRGSVVLASDATHFYANIERDRPFSIVTDLARM
                     YGAFDLVNSLADSPAHVVPGHDPLVMKRYPPAGDGLEDVAVRIA"
     gene            complement(6607..7026)
                     /locus_tag="VFJ72_01040"
     CDS             complement(6607..7026)
                     /locus_tag="VFJ72_01040"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7478076.1"
                     /translation="MATQEEKQAYIDGMVEKRGYVLDYHKVMTAQDYEVLQAANGLVE
                     AAYLKQRRLDRKTKELLFILSLTVMRASKGHIQSHIRVALDLGLSKEEILEAIEIALP
                     EAGIVAFQEGFEAWREVTGAEGIEPTIEAHQGGSGSR"
     gene            complement(7075..7911)
                     /locus_tag="VFJ72_01045"
     CDS             complement(7075..7911)
                     /locus_tag="VFJ72_01045"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7478077.1"
                     /translation="MPDLDPITGRYVYVDIDGVRNRIYFEEAGSGRPLLCLHTAGSDS
                     RQYRHLLCDPEVTSRWRVIAFDMPYHGRSTPPDGWWEKEYLLTREDYAGRIMAFLRTM
                     ELDQPVILGCSMGGAVVLELARLYGAEVGAVVALEGASKIEGRFLDWSVMPDIDGSVM
                     AASWTYGLMAPQSPESSRREVWWTYSQGGPGIYRGDTYFYSVDWDLRGREEEIDTSRC
                     PVYLLTGEYDYACSADESAATAAAIPGADFTRMEGIGHFPMAENYPRFREYLLPVLRQ
                     LE"
     gene            complement(7904..8323)
                     /locus_tag="VFJ72_01050"
     CDS             complement(7904..8323)
                     /locus_tag="VFJ72_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478078.1"
                     /translation="MSGVADLRGVFPEKEWFEALRAAVARDPELAVIGRWCDLDLALA
                     IDGELVLLRIRAGKIAETVFTPDLGASWTVTLRGTLEDWRAFLQPMPPPFYSDLLAMN
                     SRVSSFSIEGDRRTFVRHLRSIGRVFALAQRIGDKRA"
     gene            complement(8320..9222)
                     /locus_tag="VFJ72_01055"
     CDS             complement(8320..9222)
                     /locus_tag="VFJ72_01055"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7478079.1"
                     /translation="MMSKLGLIGLGNIGEPVCRNLLKGGYEVVVYDAGRAAMTRLEDT
                     GAEPAESPRELASVADVVLLSLPNSAVIEEVVLGENGLLESLSPGKVLIDTSSSKPSS
                     TRMISERLAEAGVDMLDAPVSGGVIKAREGTLAVMVGGNREVFEKCYPIFDAFGGDVF
                     FVGDHGAGHLTKALNNLLSATTLASASEAVILAERAGLSPELFVEVINASNGRSYSTE
                     VKFPKYILDRSFDDGFATALMNKDLKIALEAAAEMDFPMLIGSTVAQVWQAAVAQGNG
                     ARSHTEIFAFLENMTGGRETEDGH"
     gene            complement(9420..9575)
                     /locus_tag="VFJ72_01060"
     CDS             complement(9420..9575)
                     /locus_tag="VFJ72_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478080.1"
                     /translation="MSQSGPIVASGAMELRLSMESVIGPLVVSPDFRVLEALASSERG
                     LPLNWPV"
     gene            complement(9589..10995)
                     /locus_tag="VFJ72_01065"
     CDS             complement(9589..10995)
                     /locus_tag="VFJ72_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478081.1"
                     /translation="MASAAAGQGTLNPHAKSAIRGAWLGFFVDMFDIYLPIVVLAPAL
                     IYFVSPDLSASATAIVGGSIFAATLIGRPIGAFIFGHYADKIGRRRATIISVTGFAIG
                     TGLIALLPGYQHWGIGAVVLFILLRFIDGIFLGGEYTSASPLAMEYSPKEKRGFYGAL
                     IMTGYPLAYAAISILTLILLQFIPAGDLNSPYVQWGWRIPFILGSLMAFGLVFYYVRS
                     VHESEVFEESGGTESPLRELFRGQNLWSFLQVFVMMGGFWLTLYTASAMLPGLLTSEL
                     GMSGTRLTITLAVAFLVLAGGYMAAGVISQRVGRRPYLIANAIGMAVIGTFFYYLLIS
                     TAPKNLFAIILLVTIVVTVVTAPWGLTTTYINERFRTGVRASGFGLGYSLAVIPPAFY
                     AFYQAGLASFMPFKYTPLPLLVLGALLMLIGAAWGPETKDVDFHEDVGASPEAETHDT
                     EGVARDSRSASERGTGVS"
     gene            complement(11237..12004)
                     /locus_tag="VFJ72_01070"
     CDS             complement(11237..12004)
                     /locus_tag="VFJ72_01070"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7478082.1"
                     /translation="MNIANVRGKGVLVVGGSRGIGRAIAERFAELGAEVGLTARDAER
                     ASEVASSVAETHGQPVSGYALDVTDRSQIREVVEGFEPRSGVPDVLIYNAGISPVYAS
                     AEKVDEGDWDAIIATNLTGAFLAAQTFARRLIPESKPGSIVFIGSINSVVGDPRLVAY
                     TASKAGLAGMAKTMALDWARHGIRVNTIAPGYVTTDLTSGLQQNEGLRRSITDRTPLN
                     RMAKPAEIADLVVFLASDSSSFITGAVYPIDGGWTTG"
     gene            complement(12035..13624)
                     /locus_tag="VFJ72_01075"
     CDS             complement(12035..13624)
                     /locus_tag="VFJ72_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase family protein"
                     /protein_id="HET7478083.1"
                     /translation="MEHEHQEKVYRTELTPVSFLRRSAYVYPNKVAVVHGDRRYTYRE
                     FEERVNRLASRLRDTGLQKGDRVAFLAPNIPPLLEAHFAVPAAGCVLVPVNTRLSADE
                     VGHILKHSGSRMLFVDAELEKLVEPLDTSGIEIVRIDDTGEPGDPYEDYLAEGSPEAI
                     PSVLEDEEETIAINYTSGTTGKPKGAMYSYRGAYLNGLGVATALEMNSRSVYLWVLPM
                     FHCDGWCFPWGVTSMAATHVCLRRVESGRIWDLFDSEKVTHYCGAPTVQIGLVNHDKA
                     HRMDWQITTAIGGAPPSPTLLGQFEDLNIHPIHLYGLTETYGPTSICAWQEEWKDLSV
                     EEKAEMLARQGQAHVNSHIVRVVDDDMNDVPRDGETLGEIVTRGNTVMKGYLDNEEAT
                     EEAFKGGWYHSGDVAAWHPDGYVEIRDRSKDVIISGGENISSIEIEQVIDKHQSVLES
                     AVVAIPDEKWGERPKAFVALKPGKEATEQEIIDFCKQRLARFKAPAAVEFGELPKTST
                     GKVQKFVLREKEWAGQAKRVG"
BASE COUNT         2503 a         4595 c         4129 g         2657 t
ORIGIN      
        1 atccatgccg gccagtgtac ctgaactcct ctttcaccta cggccagtgc cttgcaactc
       61 cttgctaccg cttatagtat taagaaacat taagaaatac ggcgcagcat gatcgtgtag
      121 tttcgaccat ttctatgcga aatgcgcgct ttcagacagc cggggatcgc gtaacggcga
      181 tgttgcgaaa aggttacgga gacgttactc tctcccgcga cttgaggagg ggggctcctc
      241 tcggaagaag tccctgtgtt agtgcggtct acagagagag gaagtgttct tggttttctc
      301 gattagagta aggttgtttc tggttgcggt cgcggtggct ctggctgccg ctgtaactac
      361 cattccggcg gctacgcggc cggctgatgg tcaggtggca gcttcaggcg acgcggtagt
      421 ggcggaggcg cagacctggc tcggcgtgcc gtatgtctat ggcggttcgt cgcggtacgg
      481 ggtggattgc tccgggctca cgcaggcggt cttcggggag ttcggcgtgc cgctgccgca
      541 tagcgccgcg tcgcagttcg gttacggagt ctctgtcgcg gctccggcgg ctggcgatct
      601 ggtgttcagc gacttcaccg ggggcggtgt gggtcacgtg gggatagcga ctggcgatgg
      661 atccatgatc aacgctccgt atcccggcac caccgtgcgc tacgatccca tctcgccggc
      721 gaacgtagtc ggttacaaga gcgtcctcta gggggcgaag gagtcaggtc cgtccgctcc
      781 tcagacggtc cacaaagccg agaggccgga gcataacgct ccggcctctc gccgctccgg
      841 tcgggctggc agaggtctta ctctccggga ccctcgatct ggatgcgctt cggttcctcg
      901 atagcggcgg cacccgtcac cgtgacctca agcaccccgt cctcgaagcg ggcctggatc
      961 ttgctctcgt ccgtgccctc aggaagcgcg atgctcctgc ggaagcttcc ctggcgacgc
     1021 tcccggacgt agtagcctcc atgctgctcc tcacgctccg cgccactctt gccggagatc
     1081 gtcaacagcc cctgcgagag cgtgatgtcc acgtcttcct gcttcacgcc cggcaactcc
     1141 gcccggataa gcagatcccc atccttcgtg agaacgtcta tcgccggcgc ccacgtagcc
     1201 ggcttcgcgg acctctgccc gctcccccgc cacggcacgt tccgcatcat ctcatcgaac
     1261 aaccgatcaa actcaccctg cacatccatg aatccacgga acggactcaa catctcaaca
     1321 cctccttctc ctcggaccca tctccagggt ctacttcttc ttgcaccagc attataccaa
     1381 tttacatcca gttttcaaac atatttttga caagtttttg taaattttta cgaaaatctg
     1441 gcgtcgttct ggtatattga tgtaagaaac gaatggaggt gagtggtgtc ggatggcagg
     1501 gtgggtccga acctggcagc gatgagggag cgcaggatgt ggactcaggc gaggttggcg
     1561 cgtgaggcgg gggtaagtcc cacgacggtc tccgggatcg agggcggcag gatctcgcgt
     1621 cctcacttcg ggacgttgcg caagctggcg cgggcgctgg aggtgccggc ggaggagttg
     1681 ctcggcgaga tctcccctgg tccgttagag agtggggagc agatgtcgct ggagtgggcg
     1741 cgttccgcgc gcgaagagga gttcgaacgg cggctggacg gcgcgacgct gcacagtctc
     1801 aacgcgctct cccgcgagtt ggatgaggag cacggtcgcc tgaggacact ctacggcgag
     1861 ttcccgaaag gcagcgagca gcggcgcttc atcaagcgtc agatacggga catctcgggg
     1921 cgctcggggt cggtcggagc ttccatagag ttccacgacg actgggagga gccatgaaac
     1981 tttccggcgg ctggggcgta gagagagttg ccggaccagc gcatatttcc cccctccata
     2041 gctgagtccg gcatttcgtt ccgagggtca ggcctggagg ttgcggacgg cagccacaat
     2101 ctgagtgatc gaggcttcct tgccgaggac ggcgtcggcg cccttctcca gggcgaggtc
     2161 ctgattcttg gggttgaggt ccacggtgag cacgactatc gaggcgtcag gattcgcttt
     2221 gcgaagctcg cttatgaggt ccatgccatt gccgtccggc agccccaggt ccagaactgc
     2281 cacgtcgaac tcgctctcgc cgttcaccct gctgcgcgcc tcggccagcg acccggcctg
     2341 ggccaccacc ctgaggtccg gctccctctc cagcacgaac gccagagccc ggcgaaacga
     2401 actgtgatcc tccacgagca gaacccgctt catcctcata cctccacctc cggcgcgaac
     2461 ctctccactg tatgatcgcg tctgtgcaaa accggcacat cacgcaaatg agtgattttc
     2521 cgttgtctga caaggattgc ttacttgggg cgccgcagat cgctcatgat gtcccggccc
     2581 acctttatca ggacgagggc cccgatgggc gccgcgacga tcaagagcac cccgagcggc
     2641 tcgatcgagg gtccaaacgt gagcgcgccg accagcaaca tcaggacacc gaccgtggta
     2701 agtatggtcg agaggaccca cgcccctagc tccatcagaa gtccaaaaca tcccgaccgc
     2761 atcttcacgc tcttctcttc cacaaggaca cagcgtatcc gggaccgctc acacaggctg
     2821 cgcggcgccc ccgcgatata cgccacgcaa gaatccagac cgccccgtat ccccggcagt
     2881 ggcgggaccc ggattcgaac cggggacacc atgattttca gtcatgtgct ctaccaactg
     2941 agctatcccg ccgcgaggat gggcctcggc ccgggcctca acccgcgctg attctagagt
     3001 aagaagccta ccctatcaag ttgtgccgac cgtagtcgaa ttagcgttgc ttcgatcccg
     3061 agaaccggtt caggtcgaag gcggactcaa gcatctcttt agggagagcg cccatgacgg
     3121 ccttcggctg ctcgcctggc tcgaagagag ctatggtggg gatgctggag atctggaacg
     3181 cccccgcaag ctcttgctcc gcgtccacgt cgaccttcgc caccttcaca gagccatcgt
     3241 actcgtcggc gatctgctcc atcaccggcg ccacctgccg acacggaccg caccacgcgg
     3301 cccagaagtc taccagcacc gggatctcgg agttcaaaac ctccgcctcg aaattacttg
     3361 ccgtcacatc aaccggagcg gccatgaaac tacctcctga aaacaaactt ctaatacgtt
     3421 acttattgta accggctgtc aagaacctct cgcctcgata atagagagcg cgaaaccgac
     3481 gacagcggta accatgagta agatcgaggg cccaacggcg tgcggcacgt agtatccggc
     3541 caggctcgcg aaagccacgc ccagacagac gaaggaggcc acctgcaaga gcacgaccgt
     3601 ggtcctccgg gtacgaaggg ccacacgttc ctggcccatc tcccggacca tctccctcat
     3661 actctttccg tcatcccgcc cggattcttg cttcatcccg aaagctccaa gccgcgcttc
     3721 tccctgatag aacgccagta cgggcattct attacgccaa cggttagtct ggcctcgatg
     3781 attgctcaac gataggataa gtgtactgct gagaatagga ggcgggagcg acgggactcg
     3841 aacccgcggc ctccggcgtg acaggccggc gttctaacca actgaactac gcccccgcga
     3901 agggcgaagc agagtgtagc aaaagcggag cgcatcgtct atcgcgcgcg cgaaaagcgg
     3961 gcgataccag gttcgacccg acgggtcctt tgcaaagtgt aagacttgga tcctgcggag
     4021 cccaatcgcc ctggcggaga tggccgtcca agggctttcc ccggctcttc gtacgggctc
     4081 gtttccgagg actcgtcgtc ctcgacttca gagtgctcgc cttaccggga aagccctggt
     4141 cggtcgtatt cgctacggtc tacaggtcga cggagatgta tttggtttcc aggtactctt
     4201 cgatcccctc ggcgccgcct tcgcggccga atccggagtg cttgacccct ccgaaggggg
     4261 ccgaggcgtt ggagaccatg ccccggttga ggcccaccat gccggtctcc aggccctcgc
     4321 acactcgcag cgcccgcttg aggtcccgcg tgtatacgta ggcgacgaga ccgtactcgg
     4381 tgtcgttggc cgcctctatg gcctcctcct ccgagcttac ggagaagatc ggggccacgg
     4441 ggccgaagat ctcctcctgc cggaggtcgg cgtcctcggg cacgtcgccg agcaccgtcg
     4501 gctcatagaa gtagcccctg ccgtccatct ttccggcccc cacgagcgcc ttcgcgccct
     4561 tgctcagagc gtcgtccacg aggtccgaga ccttgccgcg ctggtcgtcg tctatgagcg
     4621 gcccgacctg gacgccctcc tccgtgccgc gcccgacctt cagggcgctc atcttctcta
     4681 ccagcttctc ggcgaactcg tccgccacgg agtcggccac gtggaagcgg ttggccgccg
     4741 tgcatgcctc gccgatgtta cgcatcttcg ccagcatcgc gccctcgacc gccgcgtcga
     4801 ggtccgcgtc gtcgaatacg aggaagggcg cgttccctcc gagctccatc gagagcctga
     4861 ggaggttgcc cgaggcctgc tccatgagct tgcgtcccac gggcgtcgag cccgtgaagg
     4921 tgagcttgcg cagacgccgg tcctgtatga ggggcgacat ggtcgcgccc gagctggagg
     4981 aggtgaccac gttgaggacg ccgggcggaa gccccgactc ctccaggatc tgcgccaggg
     5041 cgagcatcga gagcggcgtg agctgggcgg gcttcacgac gatcgtacac ccggcggcga
     5101 tggcggggcc gatcttgcgc gtacccatcg ccatggggaa gttccagggc gtgatcgcga
     5161 ggcaaggacc gaccggctgc ttcatggtga gaatccgtcc tgcgccattg ggcgcgacgg
     5221 cataacggcc gtcgatgcgc actgcctctt ccgagaacca gcgcaagaag tccgcagcgt
     5281 ataggatctc gcccttcgac tccccgatcg gcttgcccat ctccagcgtc atgagcaggg
     5341 caagctcgtc gatccgctcg accagcttct tgtaggcgcg gcgcaggatc tctcctcgct
     5401 cccgaggcgg gtgtttggcc cactccttct ggacctcgct cgcggcgtcg agcgcggcca
     5461 tggcgtcctc cggcgtggcg tcggccacct cgcagagagc ctcgccggtc gaggggtcct
     5521 ccacggggag cgtgccgcct cccgccgcgt cgcgccactc gccaccgacg aacagacgtt
     5581 tctcgacttt atcgacgatg gttctctcag ccgtgttcgc gctcatcctc gcgccgtcct
     5641 ttctctctcc ggaccctatc cctcgcagtt tagtccacat tcaccatata attctcttac
     5701 cctgccggtg gcgccacaaa cgagagacca actccgtcgc caccgcccga cagcgccgtc
     5761 ggaagactgc gtcacagtag gcaggcccgg gaggcacgat ggaggctcgg aaaccatact
     5821 atgaagtcta cgccatccgg tacgcgaccc gtgaggcgcg ctccggcgag aacttctacg
     5881 gccatgatcc ccacgatgct cccatgccca tggactattt cgtctgggcg gctgtatccg
     5941 aagagcatac tgtcgtgatc gacgctggct tcaacgccga ggtggcggcc cggcgcggcc
     6001 gggaccatct gcggtgcccg acggacggcc tgagagagct gggcatagac agcgaagagg
     6061 tcccgctcgt catcctcacg cacctccact acgatcacgt cggcaacctg gagaagtttc
     6121 ccgccgccac cttcgtggtg caggaggatg agatgaggtt ctggaccggc cggtacgccg
     6181 gcaggccgca ctttcgggag accgtcgagc ccgacgatgt gctccatctc gtgcgcgaga
     6241 acttcgctgg ccgcctgcgc ttcgtctccg gcagcgagga agtggtcccc ggcgtcgagg
     6301 tgcaccggac cggaggacat tcccccggac tgcaggtcgt aagggtcgat acggtccggg
     6361 gcagcgtggt gctcgcctcc gacgccacgc acttctacgc gaacatagag cgggaccgcc
     6421 cgttcagtat cgtcacggac cttgcccgga tgtacggcgc tttcgacctc gtcaactccc
     6481 tcgcagactc tccggcgcac gtcgtgcccg gccatgatcc cctggtgatg aaacgctacc
     6541 cacccgccgg agacggcctg gaggacgtgg ccgtccgcat cgcctgaccg gcgaaccgcc
     6601 gctcccctac ctcgacccgg agcctccctg atgggcctcg atggtcggct cgatgccctc
     6661 ggctcccgta acctcccgcc aggcctcgaa gccctcctga aaggcgacta tgccggcctc
     6721 gggcagggct atctcgatcg cttccagtat ctcctccttc gagaggccga ggtcgagcgc
     6781 gacgcggatg tgactctgga tgtgtccctt gctggcccgc atcacagtaa gactcaagat
     6841 aaacagcagt tctttggtct tgcggtccag ccggcgctgc ttgaggtacg ccgcttccac
     6901 gagcccgttg gccgcctgca acacctcgta gtcctgggcg gtcatcacct tgtggtagtc
     6961 gagcacgtaa ccgcgcttct cgaccatccc atcgatgtag gcttgcttct cttcctgagt
     7021 cgccatgtct ctcctcctgt cgcgttacac atactgccag acccggtctt gctcctactc
     7081 tagctgccgg agcacgggga gcaggtactc ccggaacctg ggatagttct ccgccatcgg
     7141 gaagtgtcct ataccctcca tcctggtaaa gtccgccccc ggtatggcgg cggcggtcgc
     7201 ggctgactcg tccgcggaac atgcgtagtc gtactcgccg gtgagaaggt agaccggaca
     7261 ccggctggtg tcgatctctt cctctcgtcc ccggagatcc cagtccacgc tgtagaagta
     7321 ggtgtcgccc cggtagatgc cggggccgcc ctgactgtac gtccaccata cctcgcgccg
     7381 ggaagactcg gggctctgcg gtgccatgag cccgtaggtc cacgacgccg ccatcaccga
     7441 accgtcgatg tccggcatca cggaccagtc aagaaagcgt ccctctatct tgctcgctcc
     7501 ttcgagtgcg accaccgctc cgacctccgc gccgtagaga cgggccaact ccagcacgac
     7561 ggccccgccc atcgaacatc cgagaataac gggctgatcc agttccatcg tccggaggaa
     7621 ggccatgatc ctgcccgcat agtcttcgcg agtgagcagg tactccttct cccaccagcc
     7681 gtccggcggc gtcgaacgtc cgtggtaggg catatcgaag gcgatgacgc gccaccggga
     7741 tgtcacttcg gggtcgcaga gcaggtgccg gtactggcgg gagtcggacc ccgcggtgtg
     7801 caggcacagg agcgggcgcc cgcttccggc ctcctcgaaa tagatgcggt tgcgaactcc
     7861 atcgatgtcc acgtatacgt accggccagt aatcgggtcg agatcaggca cgcttatctc
     7921 cgatccgttg ggcgagcgcg aagacccttc cgatagacct gagatggcgg acgaacgtcc
     7981 ggcggtctcc ctctatgctg aacgacgaca cgcggctgtt catggcgagc aggtcggagt
     8041 agaagggcgg cggcattggc tgcaagaagg ccctccagtc ttcaagcgtc cctcgaagcg
     8101 ttacggtcca ggacgcgccg agatcggggg tgaacacagt ttccgctatc tttcccgccc
     8161 ggatgcggag caggaccagt tcgccgtcta tcgcgagagc caggtccagg tcgcaccatc
     8221 gtccgatcac cgccagctcc gggtcgcgcg cgaccgccgc gcgcagcgcc tcgaaccact
     8281 ccttctccgg gaagacgcct cggagatccg ccaccccgct caatgcccat cctctgtctc
     8341 ccggcccccg gtcatgttct ccaggaaggc gaagatctcc gtgtggctcc gggcgccgtt
     8401 cccctgagcg acggcggcct gccagacctg ggcgacggtc gagccgatga gcatggggaa
     8461 gtccatctcc gcggccgctt cgagcgcgat cttcagatcc ttgttcatca gggcggtggc
     8521 gaacccgtcg tcgaaagacc gatccaggat gtacttcggg aacttgacct cggtcgagta
     8581 gctccttccg ttgctggcgt tgatgacctc cacgaacaac tccggagaga gcccggctct
     8641 ttcggcgagg atcacagcct ccgacgcgct cgcgagggtc gtcgcggaga gcaggttgtt
     8701 cagcgccttc gtcaggtgtc ccgcgccgtg atccccgacg aagaagacgt cgccgccaaa
     8761 agcgtcaaag atcgggtagc acttctcgaa gacctcccga ttgccgccca ccatcaccgc
     8821 cagggttccc tcgcgggctt ttatcacccc accgctgacc ggcgcgtcga gcatgtcgac
     8881 cccggcttcc gcgagcctct ccgagatcat gcgggtcgaa gaaggtttgg agcttgaggt
     8941 atcgatcaag accttgccgg gcgacaggct ctctagcagc ccattttctc cgagtacgac
     9001 ctcctcgatc accgccgagt tcggcagaga cagtagcact acgtccgcga cggaggccag
     9061 ttctctcgga ctctccgccg gttcggcgcc ggtgtcttcc agccgggtca tggctgcgcg
     9121 gccggcatcg tagacgacga cttcgtagcc tcccttgagg aggttgcggc atacgggctc
     9181 gcctatgttg cccagcccga tcaacccaag tttggacatc atctgagtct tccttgatta
     9241 cccacaatcg ttctacctcg caatcgaagc ctcctcacgg tggtggtgaa gcagatagca
     9301 gtagccggtg cgtcatctat gcgcgactgc tccagtcgag gacccgcatc tctttactca
     9361 atccggttcg gctctcgcta cgctcgtcta tgaaccagga acgacgccag acacgtggct
     9421 tacacgggcc agttgagtgg aagaccccgt tcggacgaag ccagggcctc aaggacgcgg
     9481 aagtcggggg agaccacaag cgggccgatc acactctcca tgctcaggcg caactccatc
     9541 gcgcctgacg ctactatcgg cccgctttgg ctcacgcccc gttgtcttct aggagacgcc
     9601 ggtaccgcgc tcagaggcgc tccgggagtc tcttgcgaca ccttctgtgt catgggtctc
     9661 cgcctccggg gacgcgccta cgtcctcgtg gaagtccacg tcctttgtct ccggacccca
     9721 tgcggctccg atgagcatga gcagggctcc cagcaccagc agagggagcg gcgtgtactt
     9781 gaagggcatg aagctagcca ggcccgcctg gtagaaagcg tagaacgccg ggggaattac
     9841 agccaggctg tatcccagac cgaagcccga ggcgcggacg ccggtacgga agcgctcgtt
     9901 gatgtaggta gtcgtcaggc cccacggcgc ggttacgacg gtcacgacta tcgtcacaag
     9961 aaggataatc gcaaagaggt tcttgggtgc cgtgcttatg agcaggtagt agaagaaagt
    10021 tccgatcacc gccataccga tagcattcgc tatcaagtac ggccggcgtc cgaccctctg
    10081 gctgatcacg cccgccgcca tataacctcc ggcgaggaca aggaaggcga ccgccagggt
    10141 gatggtgagc ctggtcccgg acatgccgag ctcggaggtg agcagtccgg gaagcatggc
    10201 cgaagccgtg tacagcgtca gccagaaacc gcccatcatc acgaagactt gcaaaaagct
    10261 ccacaggttc tgtcccctga acaactccct gagcggagac tccgtgccac cggattcctc
    10321 gaagacctcc gactcgtgca ccgagcggac atagtagaaa acgaggccga aggccatcag
    10381 gctcccgagg atgaacggta tcctccagcc ccattgcacg taaggggagt tcaggtctcc
    10441 ggccggtatg aactggagca ggatcagggt tagtatggag attgcggcgt aggccagcgg
    10501 atagccggtc atgatcaacg ccccgtagaa tccccgcttc tccttcggag agtactccat
    10561 cgccagcggg ctcgccgaag tgtactcccc gccgaggaag atgccgtcga tgaaccgcag
    10621 gagaatgaag agaacgaccg cccctatacc ccaatgctga tatcccggca gtaaggcaat
    10681 cagtcccgtc ccaatcgcga agcccgtaac ggagataatc gtcgcgcgcc gacgcccgat
    10741 cttgtccgcg tagtgtccga atataaaagc tcctatggga cgcccgatca atgtcgccgc
    10801 gaagatcgag ccgccaacga tcgcggtcgc tgaagcactg agatccggcg aaacgaagta
    10861 gatcagagcc ggagccagca ccactatggg caggtagatg tcaaacatgt ccacgaagaa
    10921 tcccagccag gctccccgta tggccgactt tgcatgagga tttagtgtcc cctgtcccgc
    10981 ggctgcgctt gccatattgc tctcctttcg acgccctgcc gtcccatcta aacggtagag
    11041 ctagcttccc cttccatgta acttatatag tgttttgcta cttcggtcaa acgtggccgg
    11101 taaactattc tgcgggaccg acctcaccga cacgcccaaa gcggcgcgca tagaaggatg
    11161 ggttgcagtc cgcgcaaacc actgcgcatg ccggttacgc tggtgcggtg cgcgttggat
    11221 caccactttc gtcacgttac ccggtggtcc accctccgtc gatgggataa acggctccgg
    11281 taatgaaact agagctgtcc gaagccagga acacgaccag gtccgcgatc tccgcgggct
    11341 tcgccatccg gttcagaggg gtacggtcgg tgatggatct ccgcaggccc tcgttctgct
    11401 gaagacccga cgtgaggtcg gtggttacgt aaccgggcgc tatggtattg acccggatgc
    11461 cgtgccgggc ccagtccagc gccatggtct ttgccatccc ggccaacccc gccttggacg
    11521 cggtgtacgc caccaggcgc ggatctccga ccacgctgtt gatcgagcct atgaaaacga
    11581 tggagccggg cttgctctcg gggatgagcc ggcgggcgaa ggtctgcgcc gcgaggaacg
    11641 ccccggtgag gttggtggct attatggcgt cccagtcgcc ctcgtcgact ttctcggccg
    11701 acgcgtacac cgggctgatg ccggcgttgt agatcaacac gtccgggacg ccggaccggg
    11761 gctcgaaccc ttccacgacc tcgcgtatct gagagcggtc cgtgacgtcc agggcgtagc
    11821 cgctgaccgg ctgcccgtgt gtctccgcca cggaagaggc gacctcggaa gccctttcgg
    11881 catccctggc cgtcaggccc acctcggccc ccaactccgc gaacctttcg gctatggccc
    11941 gcccgatccc gcggctgccg cccaccacca gcacgccttt tccccgcacg ttcgctatgt
    12001 tcatcgttgt ctcccctctg ccggccggcg atatctaacc tacgcgcttt gcctgtccgg
    12061 cccattcttt ctcgcgcagc acgaacttct ggaccttgcc ggtcgaggtc ttcggcaact
    12121 ccccgaactc cacggccgcc ggggccttga agcgggccag gcgctgcttg cagaagtcta
    12181 tgatctcctg ctccgtcgcc tccttgcccg gcttgagagc gacgaaagcc ttcggacgtt
    12241 ccccccattt ctcgtccggg atggcgacga cggcgctctc cagaacggac tggtgcttgt
    12301 ctatcacctg ctctatctct atagacgaga tgttctcgcc gccggagatg atcacgtcct
    12361 tactgcggtc ccggatctcg acgtacccgt ccgggtgcca cgcggccacg tcgcccgagt
    12421 ggtaccagcc gcccttgaac gcctcttccg tcgcctcttc gttgtccagg tatcccttca
    12481 tcacggtgtt gccgcgggtg acgatctccc cgagggtctc gccgtcgcgg gggacgtcgt
    12541 tcatgtcatc gtccaccacc cggacgatgt gggagttgac gtgggcctgg ccctggcggg
    12601 cgagcatctc ggccttctcc tcgacggaga ggtccttcca ctcctcctgc caggcgcaga
    12661 tgctggtcgg cccgtacgtc tcggtgagcc cgtagaggtg gatggggtgt atgttcaggt
    12721 cctcgaactg accgagaagg gtcggcgaag gaggggcgcc ccctatggcg gtagtgatct
    12781 gccagtccat ccggtgcgct ttgtcgtggt tgaccagtcc gatctggacg gtgggcgcgc
    12841 cgcagtagtg ggtgaccttc tcggaatcga agaggtccca gatcctgccg gattccaccc
    12901 ggcgcaggca gacgtgcgtg gcggccatcg acgtaacccc ccaggggaag caccagccgt
    12961 cgcagtggaa catcggtaac acccacaggt agaccgagcg cgagttcatc tctaacgccg
    13021 tcgcgacccc caacccgttg aggtaggcgc cccggtagct atacatcgcc cccttgggct
    13081 tccccgtggt gccggaggta tagtttatgg cgatggtctc ttcctcgtcc tccaggacgc
    13141 tgggtatcgc ctcgggagag ccttccgcca ggtagtcctc gtatgggtca ccaggctccc
    13201 cggtatcatc tatgcgcact atctctatcc cggaggtgtc cagcggctcc actagcttct
    13261 caagctccgc gtcaacgaag agcatcctcg accccgagtg cttcaggata tggccgacct
    13321 catccgcgct caggcgcgtg tttaccggga ccagaacgca cccggcggcg ggcaccgcga
    13381 agtgcgcctc aaggagcggc gggatgttgg gggcgaggaa ggccacccta tcccccttct
    13441 gcagaccggt gtcgcgcaac ctggaggcca ggcgattcac acgctcctcg aactcccggt
    13501 aggtatagcg ccggtctcca tgcacgacgg cgaccttgtt cgggtacacg tacgcgctgc
    13561 gcctgaggaa gctcaccggc gtgagctccg tccggtaaac cttctcctgg tgctcatgtt
    13621 ccatagtggc tccttccccc gtaacctacg ctacgttctt atagcccagg ctatcgttat
    13681 ccaaacgctc tggttagcat gagcttctcg ccgtgcacac ttacttatcc tgagcgattg
    13741 tccagccaac tcctcacccg cggccagaga ctcttcctgg ctccccggcc ggtcaaaagc
    13801 cccacgtgcc cggcgtcgag gacgatgaac tccttgtcct tgctgcttac gaggtccatc
    13861 accgcttcgg cctgggccaa aaag
//
LOCUS       DASSMB010000014         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_509337, whole genome shotgun sequence.
ACCESSION   DASSMB010000014 DASSMB010000000
VERSION     DASSMB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509337"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..579
                     /locus_tag="VFJ72_01080"
     CDS             73..579
                     /locus_tag="VFJ72_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478084.1"
                     /translation="MDKLERRVAEERARIEAEYRGVLRNSVQFEKRIRNRWRAKCRVK
                     NERIAALEARVGELERALEERKGVMDRGGKERKLLALMLNDMGLSDIEEPEMVRMNEE
                     VGALSEEELDRTLTSRLGLEYPASEDELEQALRGVEEPERISSEGPGGFTGAPDMSHE
                     ARPEEREG"
     gene            complement(576..2342)
                     /locus_tag="VFJ72_01085"
     CDS             complement(576..2342)
                     /locus_tag="VFJ72_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HET7478085.1"
                     /translation="MRVLDKRQEDLIARERALLDDLERFLGGFGAPREDLDLVRQTRS
                     DVEELFLLVIVGEFNSGKSAFINALLGAEVATEGVTPTTDRITLLRHADEPVERERRE
                     GILEKGYPSEFLREIAIVDTPGTNAIIRHHEELSRGFVPRSDLVLFVTSAERPLTESE
                     RGYLELIRDWGKKILLVVNKVDLLNGEENVEKVRTFIQDGIRSMLGLTPPIFFVSALL
                     ARKAKAATSDIERNALLKASGFAEIEEYVTDLLDEEGRVRLKLESPLGVVEEMIRRYS
                     NSVEERMDLLEEDFKTSENVEAQLGVYKEDMQRDFEARLAEIDNVILRMHERGDEWLE
                     ENIRLMNIRELMSQQRVQDRFQKEVVGDTEELIDERVQELIDWMVDRNLKQWRGIVEY
                     VNRRREAKYDEHVIGDVGDNFEYNRGQILNSVGKNATNVVRGYDRERESQKLAVSIQG
                     AVAQTAASGAGALGIGALVVTLFTTRFLDVTGIIAAAILAGYGLFVLPNRRRKARAEF
                     REKTGALREKLSEVVRRQFDSELNRSLERMREAIAPYTRFVRTEHARMSEAQATLKGL
                     TAETDALKGEISAPRLPEIPSS"
     gene            2441..>2804
                     /locus_tag="VFJ72_01090"
     CDS             2441..>2804
                     /locus_tag="VFJ72_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7478086.1"
                     /translation="MNYRELGKTGLEVSEVGYGAWGIGKTQWIGADDDESVRALNRAI
                     DLGLNFIDTALAYGGGHSERLVGQVVRERGEEIYVASKVPPKNGVWPAPSGLSPENVF
                     PGDYVRECTEQSLKNLGLE"
BASE COUNT          489 a          844 c          956 g          515 t
ORIGIN      
        1 actataagcg gtagcaagga gttgcaaggc actggccgta ggtgaaagag gagttcaggt
       61 acactggccg gcatggataa gttggagcgc cgggtggcgg aggaacgggc gcggatcgag
      121 gctgagtacc ggggtgtatt gcggaactcg gtccagttcg agaagcgcat caggaaccgt
      181 tggcgggcga agtgccgggt gaagaacgag cgcatcgccg cgctggaggc gcgggttggc
      241 gagttggaga gagcgttaga agagaggaag ggtgttatgg atcgcggtgg gaaggaacgt
      301 aagttgttgg cgctgatgct gaacgacatg gggctctcgg acatcgagga gccggagatg
      361 gtccgcatga acgaggaggt gggcgctctc tccgaagagg agcttgaccg gacgctcacc
      421 tcgcggttgg ggcttgagta cccggcgagc gaggacgagc tggagcaggc gttgcgcggg
      481 gtcgaggagc cggagaggat ctcgtcggag ggtcccggcg ggttcactgg cgcgccggac
      541 atgagccacg aggcgcggcc cgaggagcgg gaaggctagg aggaaggaat ttccgggagg
      601 cgtggggcgg agatctcacc tttcagggcg tcggtctcgg cggtgagccc cttgagggtg
      661 gcctgggcct cgctcatgcg ggcgtgctcc gtgcggacga agcgggtgta gggggcgatg
      721 gcctcgcgca tccgctccag tgagcggttc agctccgagt cgaactggcg gcggacgacc
      781 tccgagagct tctcccgcag ggctccggtc ttctcgcgga actcggcccg cgccttgcgc
      841 cggcggtttg gcaggacgaa cagcccgtag cccgccagga tagccgccgc gataatgccg
      901 gtgacgtcca aaaagcgggt cgtgaacagg gtaacgacca gcgccccgat gcccaaagcg
      961 cccgcgccgg aggcggcggt ctgggctacg gcgccctgga tggagacggc cagcttctgc
     1021 gactcgcgct cgcggtcgta gccgcggacg acgttcgttg cgttcttgcc gacggagttc
     1081 aggatctggc cgcggttgta ctcgaagttg tcgcccacgt ccccgatgac gtgctcgtcg
     1141 tacttcgcct cgcggcggcg gttcacgtat tcgacgatgc cccgccactg cttcaggttg
     1201 cgatccacca tccagtcgat caactcctgc acccgctcgt cgatcagctc ctcggtgtca
     1261 ccgacaacct ccttttggaa gcggtcctgc accctctgct ggctcatcaa ctcccggatg
     1321 ttcatgagcc ggatgttctc ctccagccac tcgtcgcccc gctcgtgcat tcgcaggatc
     1381 acgttgtcta tttcggcgag ccgcgcctcg aaatcgcgct gcatgtcctc tttatagacg
     1441 ccgagttgcg cctccacgtt ctccgaggtc ttgaagtcct cttccagcag gtccatccgc
     1501 tcctcgaccg agttgctgta gcgccggatc atctcctcga ccaccccgag cgggctctcc
     1561 agcttcagcc gcacgcgccc ctcctcgtcc aggaggtccg tgacgtactc ctcaatctcc
     1621 gcgaagccgc tcgccttcag gagggcgttg cgttctatgt cgctggtcgc ggccttcgcc
     1681 ttgcgggcca ggagagcgga gacgaagaag atcgggggcg tcaggcccag catcgagcgg
     1741 atgccgtcct ggatgaacgt gcggaccttc tccacgttct cctcgccgtt caggaggtcc
     1801 accttgttga cgaccagcag gatcttcttg ccccagtccc ggatgagctc taggtatccg
     1861 cgctcgctct ccgtgagcgg gcgttcggcc gaggtcacga acagcaccag gtccgaacgg
     1921 gggacgaagc cgcgggaaag ctcttcgtgg tggcggatga tcgcgttggt gcccggcgtg
     1981 tcgacgatgg cgatctcacg caggaactcg ctcggatatc ccttctccag tatcccctcg
     2041 cgccgctcgc gctcgaccgg ctcgtcggcg tggcgcaaca gggttatgcg gtcggtcgtc
     2101 ggtgtaacgc cttctgtagc gacctcggcg ccgagcaggg cgttgatgaa cgcggacttc
     2161 ccggagttga actcgcccac gatcacgagc aggaaaagct cctccacatc ggagcgggtc
     2221 tggcggacga ggtccagatc ttcgcgcggc gccccgaagc cgccgaggaa gcgttcgagg
     2281 tcgtccagga gcgctcgctc gcgggctatg aggtcttctt gtcttttatc caggacccgc
     2341 aaggcagcac ctcccttcgc cgcagattat agacaccatt tcaaatgtat accccttgcc
     2401 ctgataccat aattcggcac gcgatttgac gggagacatt ttgaactaca gggaacttgg
     2461 caagacgggt ctcgaagtat ccgaggtcgg atacggcgcc tgggggatcg gcaagacgca
     2521 gtggatcggg gccgacgatg atgagtccgt cagggctctg aacagagcga tagatctcgg
     2581 cctgaatttc atagacaccg ctctcgctta cggtggggga catagcgagc ggctcgtggg
     2641 gcaggtagtg cgggaacggg gcgaggagat ctacgtcgcc agcaaggtcc cgccgaagaa
     2701 cggcgtctgg cccgcaccct ccggcctgag cccggagaac gtcttccccg gcgactacgt
     2761 gcgcgagtgt accgagcaga gcctcaagaa cctggggctg gaga
//
LOCUS       DASSMB010000015        26854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1143309, whole genome shotgun sequence.
ACCESSION   DASSMB010000015 DASSMB010000000
VERSION     DASSMB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 26854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26854
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143309"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1359
                     /locus_tag="VFJ72_01095"
     CDS             <1..1359
                     /locus_tag="VFJ72_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7478087.1"
                     /translation="EVPVNIHYKGGWLTHEVNDCGARVAIIHEEYLPRFVEISEDLEQ
                     LEKLVVVGEGADGDAGSKLEVHSWTELADAEAISEPAATRFYDTMGILYTSGTTGPSK
                     GVVLPYGVSGIFAQAILDNAQLTSDDVGYVCNPLFHGNAQFMQILPLLLAGGRVSIWP
                     KFTVSGWLEQIRACGATITNTIGVILQFIYGQPAREDDADNPLRRVLVQPAPSDIVED
                     FEERFGVKCLEGYGMTEIGVVTYRNMDDPLRPGAAGKPLEKWFDVRIEDPETGEPLGP
                     NELGAIVVRPKSSYILMKGYHNLPEKTLEAWRDLWFHTDDMARLDKEGYLYFADRAKD
                     VIRRRGENISSFTIESILNTHPSVKEAAAFAVPSDLGQGAEDEVKVSIVAEEGEKLDP
                     AELHEYCADRMPYFAVPRYIEIVSEIPKTPNQKVRKSVLREQGVTPGTWDREAAGIKV
                     RK"
     gene            1380..2252
                     /locus_tag="VFJ72_01100"
     CDS             1380..2252
                     /locus_tag="VFJ72_01100"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7478088.1"
                     /translation="MEWNHGYVRANGIQVHHVRHGTGPPLVLLHGWPEFWYVYRKNVP
                     VLAERFDVIVPDLRGFGDTEKAGLPDPPSRLLDVLAEDLRGLADALGIDRFGLVSHDV
                     GSFVALSFARKYPKRLSGLFFFHCAYPGIGKRWLEPRSVKEIWYQSFNQQPWAASLVG
                     RDRETCKTYIKHFLDHWVHRPGLFDEDLEEWVDNFMKPGNLQGGFNWYIGINEARTEI
                     WERGAPELPKIDVPTRFFWGEEDPLIKIEWADRLDDYFSDFSFKAASGAGHFVHYEQP
                     EAANQEIIDFFSNL"
     gene            complement(2342..4090)
                     /locus_tag="VFJ72_01105"
     CDS             complement(2342..4090)
                     /locus_tag="VFJ72_01105"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7478089.1"
                     /translation="MKPDATAGTTDKVPTVATLVARFLHERGVRRFFGLQGGHIQPIW
                     DQAARLGVEIVDVRDERSAVHMAHAHADLTDEIGVAMVTAGPGVTNTVTAVANAHVSR
                     TPVLIIGGCPPRPQVNMGPLQDLPHVEIMASITRRSRTLRVGEQVLRELDEAVALASG
                     DGGEPGPVYVEIPTDVLRETISPALVLEEYLRPKPRRRTLPDPSEVERAAEILASARR
                     PAVISGRGARRAGASLVRFLNASGALYLDTQESRGLVPAEHPSIAGAVRGAVMAQADV
                     VVVVGRKLDYQVGYGSPAVFPEARLIRIADTAGELHDNRRGEVEILADTSTALDSLSA
                     ELEGRKDNLDEEWSEGLRAKHVERRESVGAALAEAPAGKDGRMHPNRIFHAIRELGLD
                     EGWIGIADGGDLLSFARTGLDARTYMDSGALGCLGVGVPFAVAAALAYPDRTVISVNG
                     DGAFGINAIDVDTAVRHGARAVFIVSNNGAWNIERHDQETNYGLVVGTELGQANYAQM
                     AQAFGAHGEQVSDPEDLPGALRRALDNAPALIDVLVTRDAVSSDATKGLGFVPDYQAL
                     TPWNDAELERRKVART"
     gene            5109..6494
                     /locus_tag="VFJ72_01110"
     CDS             5109..6494
                     /locus_tag="VFJ72_01110"
                     /inference="COORDINATES: protein motif:HMM:NF033540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HET7478090.1"
                     /translation="MTRRLPVSPSPGPLEEYAARFDHLFRARAQRDGFRRYLEGLLLP
                     AERNKTITALANAEPLAGAQRREAQSLQWFLSESGWDQEEVNERRLELLFEDRATAPD
                     GEGVLVIDEHGDRKWGKKTAHVGKQWLGNIGKTENGVVSVSSLWAEEELYYPLAVEPY
                     TPAYHFGDGKNDPKFRTKLKIASELVELAVEKEVPFRAVVADSFYGEDREFKRSLEGL
                     GVAYVLSLKKSHSWWHAPGTIGALWEDALGAGWEGAEEPGRWRKVIRTFRDGHREEWW
                     ALEVEAGPYGRERTRRALVVTPDPERLPDLATWYLTTNLPAPGSVRENEGELAAASVA
                     EVVRLYGLRMWVEQSYKQVKHALGWSAYQVRSDTAIRRHWQLACLAFSFCWWAFGRLP
                     TSSSPEEAAERPEEDLRGGRARRRNKETPGLLAGGFEGDEGVARTVGDAVALLEGVLR
                     EAPTTGAKDAA"
     gene            6628..6879
                     /locus_tag="VFJ72_01115"
     CDS             6628..6879
                     /locus_tag="VFJ72_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7478091.1"
                     /translation="MTEKGSRITEGPLRRWLYSWVRESRLKKADVQRHDLRRTFGTWY
                     LQENPGQLIELAELMGHSNLSQVRKYALSDAQRAGVAKL"
     gene            complement(7076..8176)
                     /gene="aroB"
                     /locus_tag="VFJ72_01120"
     CDS             complement(7076..8176)
                     /gene="aroB"
                     /locus_tag="VFJ72_01120"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET7478092.1"
                     /translation="MAVRIDVQVSAPYPVVIGPALDFAEALPENLESGVAAIVTDSVV
                     GPLHASSVRQGLESAGWTVAAVIEVPAGERSKSLDVYARVVRELARAGVRRDGVLFVL
                     GGGVVGDLGGFVAGSYMRGIPFVAMPTTLLAMVDSSVGGKVGLDLPEGKNLVGGFVRP
                     HAVLADLDRLSTLPPREISNGLAEVIKMALLSGGVYLEDLRLIEAARAGDADALRTLV
                     GHSVSFKADVVAEDEHETGRRAILNYGHTIGHGLEAAADYALPHGEAIAAGMVFAARL
                     SRDRYGTDLVALHEDLLHRAGLPTRPPAFDTEKVLRAMGSDKKRRSADGAAEHRFVLL
                     EDAGRPVWGVPVHEAEVRRVMGVRGNSDRRAQ"
     gene            complement(8161..8661)
                     /locus_tag="VFJ72_01125"
     CDS             complement(8161..8661)
                     /locus_tag="VFJ72_01125"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET7478093.1"
                     /translation="MSGYLTIVGYMGSGKTTVGRLIARKLGWEFVDLDHAIVREAGRS
                     IPEIFADSGEDHFRDLESLVLKKALAGPAERVIACGGGVIVRSENRERLRAANTVFLE
                     EDLDLLYGRTRGSGRPLRAADRREFEERYAARLPHYLEVADLHVEVGGRRAEQIAEEI
                     AQWLCA"
     gene            complement(8658..9836)
                     /gene="aroC"
                     /locus_tag="VFJ72_01130"
     CDS             complement(8658..9836)
                     /gene="aroC"
                     /locus_tag="VFJ72_01130"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET7478094.1"
                     /translation="MKFSFSTAGESHGPAEVALVHGVPAGLGLLADDVNRDLARRQKG
                     YGRGGRQKIEKDEIEFLGGVRHGYTLGSPIAMLVRNRDYANWESRMTPDPVEKSPDPI
                     TLPRPGHADLAGMQKYAFDDLRNVLERSSARETTARVAAGGVARKLLAEFGVGIHSAV
                     YRIGAVAYPKEQAAASARKSETSEVRCPDPEVSEKMKAEIDAARYARDALGGEFVVVA
                     EGCPPGLGSYVDWRDKLDARLAAAILSINAIKGVEFGMGFEVARHRSSEVQDEILAED
                     GGITRASNRLGGLEGGMTNGEPVVVSAAMKPISTIAKALRTVDIATGEPSRAFRERAD
                     SCAVPAAAVIGESMVAVVLAEAFLEKFGHDNMTDIRSAYDSYVSRIGWRTSEARIRER
                     "
     gene            complement(9950..10684)
                     /locus_tag="VFJ72_01135"
     CDS             complement(9950..10684)
                     /locus_tag="VFJ72_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478095.1"
                     /translation="MLSRIRDTGIARRILAIGPGFFDSFLRDNKVIPPVLAVLALLIF
                     SWVVAGIFVGKPEEERVANQANVVAQAPDNSNDSGSDSPAPQVESRDADSYAAYESKD
                     PFRQLFAPAESTNPTQETTGGSGGSTPPENTTGSGGGNSGGSTTGGGGENTSGGGGGS
                     SRGFQDSDNDGLSNRREQALGQDPQNPDTDGDGTLDGADDSNGDGIPDGQSRTTGGNG
                     GGNGGGGGTTTGKGNGGLFNSGGSLP"
     gene            complement(10674..11330)
                     /gene="pilO"
                     /locus_tag="VFJ72_01140"
     CDS             complement(10674..11330)
                     /gene="pilO"
                     /locus_tag="VFJ72_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HET7478096.1"
                     /translation="MNRNDRNVAILGALIIVVLIVGYYFLLLSPLWTRYDDAVQERSD
                     KEAQLQQLQQQVADLEKIKNDAPTVERQLLELSKRIPTQPEIPTLVVQVEEIADQAGV
                     TQISIVPGSPGPAPGGGDFSVQPITMSFEGTYDQLQDFLLRTENLARLVTVKKVSYEP
                     VEAEGTTAQAGTDPTLQVEIEADVYFQPENVPDGTAPVAPAAPQTTTPGAGGETTSAQ
                     "
     gene            complement(11330..12058)
                     /locus_tag="VFJ72_01145"
     CDS             complement(11330..12058)
                     /locus_tag="VFJ72_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478097.1"
                     /translation="MRRINLLPQEDRRRDIGGRLRSGVTGILLIAAALSVMIMVATYL
                     FFLVRLNNEEDKIADLDTQIAQQNQRLAELAPYRDLQARLDAKKPIADGIFRTRFPWN
                     DFLQGLAFVIPPSTALDTLTAEAAPINIDAPAEQPLNPPGAVTFTGVSLPQYTNVADF
                     VVQMNNLRFLANSQLDSAELDRDTFSQDAINFEVAAELITEVGSKGTELPLDSGSDSS
                     GGSEATTQTQDATGSQASGEDGRR"
     gene            complement(12055..13443)
                     /gene="pilM"
                     /locus_tag="VFJ72_01150"
     CDS             complement(12055..13443)
                     /gene="pilM"
                     /locus_tag="VFJ72_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HET7478098.1"
                     /translation="MGRFRTQTIGLDIDRGSLKAVQVSRGSGGYTLQHVGYHRLPPGT
                     IADGEVADHDLLAAEIKEFWDSHSFKGKSVILGLSNQKVVVRLVDFPRMSPEDLKTAI
                     GYEAQDHIPMPLEEAVMDYVVLGPAAEGSDLDRILIVAAQREMISRYTSAARAGGLRP
                     VGVDVKALALTRSVLPQGGYDAEGAVLIMDIGSELTNLVVYQGGSPELTQFVPGGSLY
                     FIQAIADAADIPDEEAEKHLMNPRVRLGYALDAEQTQEGPHSEPDTRETPLDARSTGE
                     DTAELDARDTAFADEETAELRDPESERVTPPDAAEQSGEERSEAGWEPEAGATENGPE
                     PDGAEETAAEEPETSVEGPEYQWDPALDYDVRRGLEDAVGLLAEDVQRSIDYHYSQPG
                     ARELSQVFVTGEGALVAGIDDYLSELLGIHTLRGNPLSKIVANRSNVSDEQVRVMEPV
                     LAVALGLAMEES"
     gene            complement(13450..14199)
                     /locus_tag="VFJ72_01155"
     CDS             complement(13450..14199)
                     /locus_tag="VFJ72_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET7478099.1"
                     /translation="MITVLAALLGLLVGSFLNVVIHRVPRHESIVWPSSRCPKCGAEI
                     SPLDNVPVLSYVLLRGKCRNCGARISARYPLVEALTGVLFGVAAWRFGIGAELAGALV
                     LIAVLVALAGIDLEHRLLPNVIVGPAALAGLLLSTLAAPAMWWVYLVSAVAVAGGLLG
                     LALVYPGGMGMGDVKMGGMLGAFLGPYAALAVFIGALVGALVGGVLISTGKMRRRTAM
                     PFGVFLALGGVVALLAGREMWSFYLDFAGGA"
     gene            complement(14196..14813)
                     /locus_tag="VFJ72_01160"
     CDS             complement(14196..14813)
                     /locus_tag="VFJ72_01160"
                     /inference="COORDINATES: protein motif:HMM:NF036822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478100.1"
                     /translation="MERVEIEEELRVAIVGAGGFGRVALDVLLASGSERWVMGFYDDA
                     HSVLADKVRGFPILGDIGMLKSMLSVEPVHIIVAITDNEARLRIANSIRALGGQFVTA
                     LHPLAYVSEVAVVGDGTVVAASAVVHPDAVVGSHCYVGPGAVIDRDSEVGAGAWIAAG
                     AIVGSGARIGARAVLGHSVSVGRKATVRANARLESLSRIPEGDSV"
     gene            complement(15009..15929)
                     /gene="fmt"
                     /locus_tag="VFJ72_01165"
     CDS             complement(15009..15929)
                     /gene="fmt"
                     /locus_tag="VFJ72_01165"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET7478101.1"
                     /translation="MKLAFAGTPQFAATILSGLLESDHEVGLVISQPDARRGRGRRVA
                     STPVADLAHARDLPLRQPERISEAAAEISDHDALIVAAYGQILRPDTLYAASHGAWNV
                     HASLLPAYRGAAPVERAIMAGEQRTGVTIMQMDEGLDTGPMALQRETAIPADMTGGEL
                     TELLARLGSEAVVESMSLLETGSLTLTEQDSLKATYAAKLSNEDRVIKWDIGADEVHN
                     IVRALAPYIGARTFHEEYDGPVRILRSRIAYESEVSLRPGEIYAANGRIFVGCGRGVV
                     EVLEIQAPGGRALPAANFLRGNALGGRFRV"
     gene            complement(15942..16436)
                     /gene="def"
                     /locus_tag="VFJ72_01170"
     CDS             complement(15942..16436)
                     /gene="def"
                     /locus_tag="VFJ72_01170"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7478102.1"
                     /translation="MALEMRTFGDPILKSRAAPVKSFDESLARLADEMLSTMREQEGV
                     GLAANQVGRLKRIFVAGTEDEEYVIVNPEIESSSEDTVTDAEGCLSIPGISVEVERPA
                     SVSVSGQSVTGEPLRIEAAGLLARVMQHEIDHLNGVLILDRTDRESRKAAMRELRERL
                     LASQ"
     gene            complement(16440..18665)
                     /locus_tag="VFJ72_01175"
     CDS             complement(16440..18665)
                     /locus_tag="VFJ72_01175"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478103.1"
                     /translation="MSGQRSRDAAARVCLLIPTHALPPLSYRVPATLAREVRMGSMVV
                     APLKQHSRIGVVVGFEYTAEHELEEIYSVAEGLSLREEMVDLCGWISENTALPLAVVV
                     RAALPPGMDTGSYRVSGPLAGWPWREGALVRRAVARRRLGAAGLVAAEREKRLELVPV
                     LPAPEETQWATLLSGANPGFKWTPKQKELLGSLMDRGAEYPAADLLSETGASRVTLTS
                     LVRRGVVRLEQRDGPSAVWATRGGTEDSGGDPATGEMLRVLDGGACLHRVPSKDTFSE
                     VAALSRSAVEQGRQVLVLVPERETAERLAGCLRRELRTGYSVAAYHSAVGRNRGVIYR
                     CAAKGEVDVLIGTRTAALLPMRRLGAICVVDEPNEAHRAEPGYEGLPLHVREICLKRG
                     ELEGAGVLFLSPAPSLRLFASKDRVRELPARLPEDWPAVRIVDMRGSGAALSSTLIEA
                     CRHHIQDGKRVGVLVNRTGYATAVACTSCGAVRSCPVCDIPLVPYDESRRLVCPRCAY
                     AEEAPRTCSACGSERMSPTGLAVERAREVLSEALGEPVGLVTARESRRSDAPVVVGTA
                     RYILGRDWDVVMIPDNDGLLLGSGVGAVERAFRRLYFAAEAAAEYLYVQTRVPEHYAL
                     RAAVRADYPGFASAELPRLLSLGYPPFAHLADVTLEGAEEVVRRAVESGLRSGLETGV
                     RMTGPVPVSRTGDSPAIWRVLLRGRERAPVARGGALAARLATGTRGGMKVRVGIDPEE
                     V"
     gene            complement(18686..19924)
                     /gene="metK"
                     /locus_tag="VFJ72_01180"
     CDS             complement(18686..19924)
                     /gene="metK"
                     /locus_tag="VFJ72_01180"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7478104.1"
                     /translation="MAEKTVGTTTWNAADDQKLRASHLFTSESVTEGHPDKIADQISD
                     TILDAALADDPMSRVACETLVTTGLAIIAGEITTSTYVDIPTLVRQKVAEIGYTRAKF
                     GFDAETCGVMVSIDPQSADIAQGVDKALEVRETEENIDEIGAGDQGMMFGYACEETDE
                     LMPLPITLAHALTRRLSEVRKSRQLDYLRPDGKSQVTVRYEGDTPVGIEKILISAQHR
                     DGMEHHIRDDLIETVIRPVMSDHLFDESVAEILINPTGRFVTGGPQGDTGLTGRKIIV
                     DTYGGAAPHGGGAFSGKDATKVDRSGSYAARWVAKNVVAAGLARRCMVQVAYAIGVAH
                     PVSVMVETFGTASVDPVTISGLVQEHFDLRPGAIIRDLDLRKPVFARTAAYGHFGRRE
                     PGFTWEDTSRADALARAAGL"
     gene            complement(19925..21022)
                     /gene="coaBC"
                     /locus_tag="VFJ72_01185"
     CDS             complement(19925..21022)
                     /gene="coaBC"
                     /locus_tag="VFJ72_01185"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET7478105.1"
                     /translation="MILLSVGPASGALSAPNIYRELIGSGQQVEIFLEPGTTNFVGHA
                     AFGGYAATSDDEEMYSSVIFAPATAGTIARLANGLAEGKAGEAYVAGLRPAYVVPDLD
                     PMTARHPAVAENLALLRADDCKVLEGTDGKMAAVGEVVAAVLGGMNGPLSGLRVVVTA
                     GGTREPIDSVRFVGNRSSGKMGLAVAREARRLGAEVAVVAANVERAEPGVEWHPVETV
                     DEMRRSVMDLVAGADVLVMAAAVSDFKPATPMQAKVRRSEGLSVEFVATEDILAAVRE
                     RNRDLFMVGFAATHGDPVPDAREKLSKKGVDLVVGNDISRSGIGFGSEENEVYVVSRD
                     GERLVPRASKTEIARAILRDIMAEIERERRD"
     gene            complement(21062..21445)
                     /gene="rpoZ"
                     /locus_tag="VFJ72_01190"
     CDS             complement(21062..21445)
                     /gene="rpoZ"
                     /locus_tag="VFJ72_01190"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET7478106.1"
                     /translation="MLNELKIDELLEKVDSRYGLVVSSAKRARQINEYTATLGLNDAL
                     GIPGPQVHTRSQHPLSIAIEELRQDKLEVTFRDPADEAEEQAEEQVGAADGSDFVDPP
                     SGESAPATAEVDTGAPEDEGDQGAA"
     gene            complement(21429..22010)
                     /gene="gmk"
                     /locus_tag="VFJ72_01195"
     CDS             complement(21429..22010)
                     /gene="gmk"
                     /locus_tag="VFJ72_01195"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7478107.1"
                     /translation="MRRGKLIVVSGPSGAGKSTLIRQALDTVPELAYSVSATTRKPRP
                     GEVNGQHYIFLSREEFERWIEAGYFLEWAEYSGNLYGTPEHRVDELMDAGLSVILEIE
                     LQGARKVREKRPDAVMVFVRAPSLEETRRRLEGRATETSAAVESRMTTALKEVAAREE
                     FDHEVINADRKQAREDMIGLMRNIVGGEDAERT"
     gene            complement(21992..22318)
                     /gene="mihF"
                     /locus_tag="VFJ72_01200"
     CDS             complement(21992..22318)
                     /gene="mihF"
                     /locus_tag="VFJ72_01200"
                     /inference="COORDINATES: protein motif:HMM:NF041260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HET7478108.1"
                     /translation="MLRRAPQRTSQERRRALEEANRVRFTRAATKRDLKSGELNIYDL
                     LMDPSEELKGAKVEEMLLSVRGMGRIKVTRILREAGVSRSKTLVGLTFGQRDRLIRAL
                     GCVEEN"
     gene            complement(22579..24075)
                     /locus_tag="VFJ72_01205"
     CDS             complement(22579..24075)
                     /locus_tag="VFJ72_01205"
                     /inference="COORDINATES: protein motif:HMM:NF005303.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7478109.1"
                     /translation="MTGHAVVIAGGGPTGLMLAGELALAGVDVAIVERRASQELPGSR
                     AGGLHARTIEILDQRGIADRFLSEGQVAQVAGFASVRLDISDFPTRHPYGLGLWQNHI
                     ERILAGWVAELKVPIHYGTEVTGFAQDDTGVDVQLSDGRALRAHYLVGCDGGRSLVRK
                     AASIDFPGWDPTTSSLIAQVEMAEEPELGIRRTPSGMHALGKVEYEIKDGEVVYEKGG
                     TVGVMLTESEIGTSEPTLRDLSEGLIAAYGTDYGIHSPTWISRFTDMTRQAAAYRDRR
                     VLLAGDAAHVHSPVGGQGLNTGVQDAVNLGWKLAQVVKKTSPDSLLDTYHAERHPVGA
                     RVMRNTMAQVALMRADDRIEALRDTVSELLSMDEPRKRFAAEMSGLDIHYDLGEGHPL
                     LGRRMPDLDLVTTGGPLRVFTLLHDARPVLLNLGEPGGFDITPWADRVQLIDAKYVGT
                     WELPVLGQVAAPAAVLIRPDGYVAWVGDGTDTGLRDALTTWFGPPDAA"
     gene            complement(24739..25629)
                     /locus_tag="VFJ72_01210"
     CDS             complement(24739..25629)
                     /locus_tag="VFJ72_01210"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET7478110.1"
                     /translation="MREQATGVDLAVELCGLQLATPLIPASGTLAREALGEVLGVYGA
                     MLPKTTTPVPRAGNPPPRIAETPAGMVNSIGLQNPGVEGFLEVLDAFDVGVPLFVSVA
                     GDTVDEFAALCERISGDARISGIELNLSCPNVEHGGLTFCAGPASVEEVVAACRPVVQ
                     DKLVFAKLTNEGVVENARAAEAAGADALTVINTLPALTIDSRARKILVRGGLSGPAIK
                     PVALRAVYDVSRAVKVPVIGSGGAASGTDVAEFMLAGATAVQIGTASFVREPSEILGE
                     FSAYLKEMGLAASDLAGTLR"
     gene            complement(25629..26363)
                     /locus_tag="VFJ72_01215"
     CDS             complement(25629..26363)
                     /locus_tag="VFJ72_01215"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET7478111.1"
                     /translation="MRFYSVEIEERREIGGYTLLSYRWPEGSVEPGQFVMARAAGYPI
                     TFDPFLARPFSFYDYEGGVASLLFEVRGRGTALLDRAPERIEVSAPLGRGFGIQPTAE
                     RSALLGGGIGVAPLKILSRRLTALNIPHDIFLGFAEARFVPVAGEFPSAKVATVDGSS
                     GTRGTVLDVVPDLEGYDAVYACGPNPMLAAVKAAGRDSVCQLSVEERMGCGNGSCNGC
                     VVPVRGGGYVRSCVEGPVFDAEVLAW"
     gene            complement(26360..>26854)
                     /gene="carB"
                     /locus_tag="VFJ72_01220"
     CDS             complement(26360..>26854)
                     /gene="carB"
                     /locus_tag="VFJ72_01220"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HET7478112.1"
                     /translation="STGEAMGIDATFGGAFAKALTAVGLTLPVAGRVYISVSNRDKRS
                     VVLLARAFADLGFEIAASEGTAEVLQNNGLPVTVVPKIGELGEDVLGLIEDGGVDLIL
                     NTPWGRGARTDGYLIRRKALLHGVPCITTLAAAAAAVQGIESKIRGGMRRVNPLQGLY
                     ATKT"
BASE COUNT         4659 a         9185 c         8339 g         4671 t
ORIGIN      
        1 gaggtccccg tcaatatcca ctacaagggg ggctggctca cccacgaggt gaacgactgc
       61 ggggcgcgag tggcgatcat ccacgaggag tacctcccca ggttcgtgga gatctccgaa
      121 gacctggagc agctagagaa gctcgtcgtg gtgggcgagg gcgccgacgg ggacgcgggg
      181 agcaaattag aggtgcattc gtggaccgaa ctcgcggacg cggaggcgat atccgagccc
      241 gccgcgacgc ggttttacga cacgatgggc atcctgtaca cgtccggcac cacggggcct
      301 tccaagggcg tcgtgctccc gtacggtgtc agcggcatct tcgcccaggc gatcctggac
      361 aacgctcagc tcacaagcga cgatgtcggc tacgtctgca acccgctctt ccacgggaac
      421 gcgcagttca tgcagatcct gccgctactg ctcgccgggg gtcgcgtcag catctggcca
      481 aagttcaccg tctccgggtg gctcgaacag atccgggcgt gcggcgcgac tattacgaac
      541 acgatcgggg tcatactcca gttcatctac ggccagcccg ctcgcgagga cgacgccgac
      601 aacccgctgc ggcgagtgct cgtccagccg gcgccgagcg acatcgtcga ggacttcgag
      661 gagcggttcg gggtgaagtg cctggaaggc tacggcatga cagagatagg cgtcgtcacg
      721 taccggaaca tggacgaccc actgcgtccc ggcgcggccg gcaaaccgct ggagaagtgg
      781 ttcgatgtca ggatagagga ccccgagacg ggcgagccgc tcggcccgaa cgagctaggc
      841 gcgatcgtgg tgcgccccaa gtcttcgtac atcctcatga agggctacca caacctcccc
      901 gagaagaccc ttgaggcctg gcgcgacctg tggttccaca ccgacgacat ggccaggctg
      961 gataaggagg gctacctgta cttcgccgac cgagcgaagg acgttatccg gcgccgggga
     1021 gagaacatct cgtcgttcac catcgagagc atcctgaaca cccatccttc ggtgaaggag
     1081 gccgccgcgt tcgctgtccc gtcggacctc ggacaggggg cggaggacga ggtcaaggtc
     1141 tccatcgtgg ccgaagaggg agagaagctg gacccggcag agctgcacga gtactgcgcg
     1201 gacaggatgc cctacttcgc ggtcccccgc tacatcgaga tcgtctcgga gatacccaag
     1261 acgccgaacc agaaggtcag gaagagcgtc ctgagggaac agggggtcac gcccggcacg
     1321 tgggaccgcg aggcggcggg catcaaggtg cggaagtagg agcagaggcg acggccggga
     1381 tggagtggaa ccacggatac gtccgggcga acgggatcca ggtccaccac gtccgccacg
     1441 ggaccggccc gccgctcgtg ctgctgcacg gctggcccga gttctggtac gtctaccgga
     1501 agaacgtccc cgtcctggcc gaacgcttcg acgtgatagt gcccgacctg cgaggcttcg
     1561 gcgacaccga gaaagccggc ctgcccgacc ctccgagccg gctcctcgac gtgctggcgg
     1621 aggacttgag agggctcgcg gacgcactgg ggatagaccg cttcggcctg gtgagtcacg
     1681 atgtgggctc gttcgtcgcc ctgtccttcg ccaggaagta cccgaagagg ttatcggggt
     1741 tgttcttctt ccactgcgcc taccccggca tagggaagcg ctggctggag ccgcggagcg
     1801 tcaaggagat ctggtaccag tccttcaacc agcagccgtg ggccgcctcc ctcgtcggca
     1861 gggaccgcga gacgtgcaag acgtacataa agcacttcct ggaccactgg gtgcataggc
     1921 ccggcctgtt cgacgaggac ctggaggaat gggtggacaa cttcatgaaa ccgggcaacc
     1981 tgcagggcgg cttcaactgg tacataggca ttaacgaggc gaggaccgag atctgggagc
     2041 gcggggcgcc ggagctgccg aagatagacg taccgacccg cttcttctgg ggcgaggagg
     2101 acccgctgat caagatcgaa tgggcggacc ggctcgacga ctatttctcc gacttttcct
     2161 tcaaagccgc ctcgggcgcc gggcacttcg tccactacga gcagcccgag gcggccaacc
     2221 aggagatcat agacttcttc tctaacctgt aacccggacg gacagagcga gaccctgtgt
     2281 tctcccggcc tccggctcta aggcttcgtt cgtagacgcc gggagagcct cgcgcccgct
     2341 ctcaggtacg cgccaccttg cggcgctcca gttccgcgtc gttccacggg gtgagcgcct
     2401 ggtagtcggg cacgaaaccg agacccttcg tggcgtcgga cgagacggcg tccctggtga
     2461 cgagcacgtc gatcagggcg ggcgcgttgt ccagggcgcg ccgcagggcg ccgggcaaat
     2521 cctccgggtc gcttacctgc tcgccgtgcg cgccgaaagc ctgggccatc tgcgcgtagt
     2581 tagcctgtcc caattcggtg ccgaccacca gaccgtagtt ggtctcctgg tcgtggcgct
     2641 cgatgttcca cgcgccgttg ttggagacga taaacacggc gcgcgccccg tggcgcacgg
     2701 ctgtgtcgac gtcgatcgcg ttgatgccga acgccccatc gccgttgacg gagatcaccg
     2761 tcctgtccgg gtaggcgagc gccgccgcga ccgcgaaggg caccccgaca ccgagacaac
     2821 ccagggcccc ggagtccata taggtcctcg cgtcgaggcc ggtgcgcgcg aagctcaaca
     2881 ggtcgccgcc gtcggcgatg ccgatccaac cttcgtccag gcccagctcg cggatggcgt
     2941 ggaagatgcg gttcgggtgc atgcgcccgt ctttcccggc cggcgcctcc gccagagccg
     3001 cgccgacgga ctcccgccgc tcgacgtgct tggcccgcag tccctcgctc cattcttcgt
     3061 ccaggttgtc tttccggccc tcgagttcgg cggagagtga gtccagcgcg gtggaggtgt
     3121 ccgcgaggat ctcgacctcg ccgcggcggt tgtcgtggag ctctcccgcg gtgtcggcga
     3181 tgcggatcag gcgagcctcc gggaagacgg cgggcgaccc gtagccgacc tgataatcca
     3241 gcttgcgccc gaccacgacg accacgtcgg cttgcgccat gacggcgccc cgcaccgcgc
     3301 ccgctatcga cgggtgctcg gcgggcacca gcccccggct ctcctgagtg tcgaggtaca
     3361 acgccccgga ggcattcagg aaccttacga gcgacgcgcc cgcccggcgg gctccccgtc
     3421 cggagatcac ggccggacgc cgggcggagg cgaggatctc ggcggcccgc tccacctccg
     3481 acgggtccgg cagcgtcctc ctgcgcggct tcggccgcag atactcctcg agcaccaggg
     3541 ctgggcttat ggtctcgcgc agcacgtcgg tggggatctc cacgtagacg ggacccggct
     3601 cgccgccgtc gccggaggcg agagcaaccg cctcgtctag ctccctgagc acctgctcgc
     3661 ccacccgcag cgtgcgcgac cggcgagtga tcgacgccat gatctcgacg tgcgggaggt
     3721 cctgcagcgg ccccatgttc acctgcgggc gagggggaca gccgcctatg atgagtaccg
     3781 gtgtccgcga aacgtgggcg ttcgccaccg ccgtgacggt gttggtaacg ccgggacctg
     3841 cggtcaccat cgccacgccg atctcgtcgg tgaggtccgc gtgcgcgtgg gccatgtgaa
     3901 ccgccgaacg ctcgtcgcga acgtcaacga tctctacgcc caaccgtgcc gcctggtccc
     3961 agataggctg gatgtggccg ccttgcaggc caaagaaccg ccgcacaccg cgctcatgca
     4021 ggaaccgcgc gacgagggtg gcaacagtcg ggactttgtc tgtggtccct gccgttgcgt
     4081 caggcttcat ttcggcacct ttctgtcaaa tgcgctcgtt gacccggcag cgactgtagc
     4141 catcgccaag cccggcgacc cactaaggcc cgcctgcgcg cgtgctatgt acgccgccgc
     4201 acactaatgg ctaaattcaa aagctgttac tgcctccatc aactctttcc taattcttag
     4261 gcttggacct tcaacccaag cccaccccag ttggacgatt cgattatcga gaatcatgta
     4321 gaaatttccc ccatttctac cgaaatattt tgtcaaaata agcactggct gtcaaccgag
     4381 cgcacgctca gtaactgttc tccatatgaa gaggaggtaa cctcgccgta atgtgtgggt
     4441 tctcccgaac tttaggtgag aaggtagctt cggggtttcg gctccatgag aaagcgttca
     4501 ggtcgtcagg attctggcct tcagcagggc gaaagtagat caatgccact cacctgaaga
     4561 ggaagtgcgg tagatgctac aggtctcggc acgtgtccta accgtgctct aaaggtccgc
     4621 gaggggcgcg aagactatga tcaccaacca aatgagactg gcaaatagcg caacctctgc
     4681 tccctcgaga cggtaggcgg tggaggccac gacgaggctc gctcgatgct ggtggcgatc
     4741 tacggcgaca cttaagtacc ggctcagaag cagtagcatc cgtcttcgat actctgtact
     4801 tctgccatca ccgctccacc tttagaacgc taatgatggc gaggttacct catctaccgg
     4861 aacccgatag aatctcgggc gtttcaggat tgacgagccc gaggggcgtc cgccgaacga
     4921 taaaggacgt tgcgtgccgc cattttcgct atccacattg gacaaggtca cggcccggga
     4981 tgtcatgggg tccttcgcgc actacggcta ccgcacgatg gaccaactgc cgtgacagat
     5041 gctctaacgg tagtttgttg aagtaggaac ggtcttgtgc tccaatggcg gcattaggag
     5101 gtgccgccat gaccagaagg ctgcccgtct caccgtctcc aggcccgctg gaggagtacg
     5161 ccgcccgctt cgaccatctc ttccgtgctc gtgcccagcg cgacggcttc aggcgctacc
     5221 tggaggggct cttactgccc gccgagcgca acaagaccat aaccgccctg gccaacgccg
     5281 agcccttggc cggggcgcag cgaagggagg cccagagcct gcagtggttt ctctcggagt
     5341 ccggctggga ccaagaggag gtcaacgagc gccgcctgga gctcctgttc gaagaccggg
     5401 cgaccgcccc cgacggggag ggggtgctgg tcatagacga gcacggcgac cgcaagtggg
     5461 gcaagaagac cgcccacgtg ggcaagcagt ggctgggcaa catcggcaag accgaaaacg
     5521 gggtggtgag cgtcagcagc ctgtgggcgg aggaggagtt gtactacccg ctggctgtcg
     5581 agccttacac tccggcgtac cacttcgggg acggcaagaa cgaccctaag ttccgcacca
     5641 agctgaagat cgcttcggag ctggtggaac tggccgtgga gaaggaagtc cctttccggg
     5701 cggtcgtggc ggactccttc tacggcgagg accgtgagtt caaacgctcc ctcgaaggac
     5761 tcggcgtagc ctacgtgctt tcgctcaaga agtcgcactc gtggtggcac gccccgggca
     5821 cgatcggtgc cctgtgggag gacgcccttg gggccggatg ggaaggcgcc gaggagccgg
     5881 ggaggtggag gaaggtgata cgcaccttcc gcgacgggca tcgggaggag tggtgggctt
     5941 tggaggtgga ggccggtccc tacggtaggg agcggacaag aagagcgctc gtggtcaccc
     6001 cggatcccga aaggcttccc gatcttgcca cctggtacct gacgaccaac ctgcccgccc
     6061 ccggttccgt gcgggagaac gagggcgaac tcgccgcggc gagcgtggcg gaggtggtga
     6121 ggctctacgg gctgaggatg tgggtcgagc agagctacaa gcaggtcaag cacgccctgg
     6181 gctggagcgc ctaccaggtc aggagcgaca cagcgatccg ccgccactgg cagctggcgt
     6241 gcttggcgtt ctcgttctgc tggtgggcct ttgggcgcct gccgacttct tcttcgccgg
     6301 aggaggcagc cgaacgaccg gaggaagacc tacgcggcgg gcgggcaaga agaaggaaca
     6361 aagagacccc gggcctcttg gccggaggct ttgagggcga tgagggcgtg gctagaaccg
     6421 tgggtgatgc tgtggcgcta ctggagggcg ttctccgaga agcccccacc accggagcta
     6481 aggatgctgc ttgagcgggt gttctcgggg caggggctct acctctatgc ccgctaacaa
     6541 caaactaccg ctaaggcgct acctgaaggc ccgaaacgcg ctcgttaccg tcagcgaagg
     6601 tacgggatcc cccttcccca ccttttcatc accgagaagg gctcgcgcat cacggaaggc
     6661 cccctacgca ggtggctcta ctcttgggtc agggagagcc gcctgaagaa ggcggatgtg
     6721 cagcgacacg acctcagacg cacgttcggg acctggtacc tgcaggagaa tcccggccaa
     6781 ttgatcgagc tggccgagtt gatgggacac tcgaacctct cgcaggtcag gaagtacgcg
     6841 ctttcggacg cgcagcgggc aggggtagcg aaactctagc cgctagtttg acggacccga
     6901 gcactgggcc ggatagtgca agagtagtgg gtccaggcgg cagaagtgat tacgccacca
     6961 caggccaacg tgattcctac acccggaaac gcgcttcccg gcagcctcgc gacgccatcc
     7021 agagtaagaa ccatggacct ctggtctacg ttgatctaca gttccgaggt tcagttcatt
     7081 gagcacggcg gtccgaattt cctctgactc ccatcacgcg gcgcacctcc gcctcatgca
     7141 ccggtacacc ccagaccggc cgtccggcgt cttccagcaa cacgaagcgg tgctctgcgg
     7201 cgccgtcagc ggagcggcgt ttcttgtccg agcccatggc ccgcaacacc ttctccgtgt
     7261 cgaaggccgg cgggcgggtc ggaaggccgg cgcggtggag gaggtcctcg tgcagcgcta
     7321 cgaggtcggt gccatagcgg tcgcgggaga gcctggcggc gaagaccata ccggccgcta
     7381 tggcctcgcc gtgcggcagg gcgtagtcgg cggcggcttc gaggccgtgg ccgatggtgt
     7441 gaccgtagtt caggatggcg cggcgcccgg tctcgtgctc atcctcggcg actacgtcgg
     7501 ccttaaaaga gacggagtgc ccaacgaggg tacggagggc gtccgcgtcg ccggctcggg
     7561 cggcttcgat caggcgcagg tcctccaggt agacgccgcc ggagagcagg gccattttga
     7621 tcacctcggc gaggccgttg gagatctcgc gcggcggcag ggtggagagc cggtccaggt
     7681 cggcgagtac ggcgtggggc cgtacgaaac ctccgacgag gttcttccct tccggaaggt
     7741 cgagcccgac cttgccgccg accgagctgt caaccatcgc gagcagagtg gtaggcatgg
     7801 cgacgaacgg tatgccccgc atgtaggaac ctgccacgaa gccgccgagg tccccgacga
     7861 cgcctccccc aaggacgaat aggacgccat cgcgtctcac tcccgcccgc gccaactcgc
     7921 gcacgacgcg agcatacaca tcgaggctct tggagcgctc ccctgccggc acctcgatga
     7981 ccgccgccac ggtccagcca gcggattcga ggccctgccg gaccgatgag gcgtggagcg
     8041 gaccgaccac ggagtcggtc acgatggcgg cgacaccaga ctccaagttc tccgggagag
     8101 cctccgcgaa gtccagtgct ggtccgatga cgaccggata aggcgccgag acctggacgt
     8161 ctatgcgcac agccactgcg cgatctcctc ggcgatctgc tccgcgcgcc ggcctccaac
     8221 ctcgacgtga aggtcggcga cctccaggta gtgtggtagg cgtgccgcgt agcgttcctc
     8281 gaactcccgg cggtccgcgg cccgcagcgg gcgtccgctg ccgcgagtcc tgccgtagag
     8341 gaggtccagg tcctcctcca ggaacacggt attcgcagcg cgcagccgct cgcggttctc
     8401 tgaccgcacg atcacacccc cgccgcacgc gataacacgc tccgccggtc ccgccagggc
     8461 cttcttcaac accaatgatt cgaggtccct gaagtggtct tcaccggagt cggcgaagat
     8521 ctccggtatc gagcgtcccg cctcgcggac tatggcgtgg tcgaggtcta cgaattccca
     8581 tccgagcttg cgggcgatga gacggcccac ggtggtcttt ccagacccca tatatccgac
     8641 gatggtcaag taaccgctca acgctcccgg attcgcgcct cgctggtcct ccagcctatg
     8701 cgggacacgt acgaatcgta ggcggatcgg atgtccgtca tgttgtcgtg gccgaacttc
     8761 tcgaggaacg cctcggccag caccacggct accatcgact ccccgatcac ggccgccgcg
     8821 ggcacggcgc aggagtcggc cctctcgcgg aaggcccgcg aaggctcgcc ggtcgcgatg
     8881 tccacggtcc tgagcgcctt ggctatggtg gagatcggct tcatcgccgc gctcaccacg
     8941 acgggttctc cgttggtcat gccgccttcg agtccgccga gccggttgct cgcgcgcgta
     9001 atgccaccgt cttcggccag gatctcatcc tgcacctcgc tggagcggtg gcgggcgacc
     9061 tcgaagccca tgccgaactc cacgcccttg atggcgttta tcgagaggat tgcggcggcc
     9121 aggcgggcgt cgagcttgtc gcgccagtcc acgtaggagc cgaggcccgg cggacacccc
     9181 tccgccacca cgacgaactc tccgccgagg gcgtcgcggg cgtagcgggc ggcgtcgatc
     9241 tccgccttca tcttctccga gacctccggg tccgggcagc ggacctcgga ggtctcggac
     9301 ttgcgggcgc tcgcggcggc ctgctccttc gggtacgcga cggcgccgat gcggtagacg
     9361 gcgctgtgga tcccgacccc aaactccgcg aggagcttcc gcgccacgcc accggcggcg
     9421 acgcgggccg tcgtctcccg cgcgctggag cgttcgagaa cgtttcgcag gtcgtcgaag
     9481 gcgtacttct gcatccccgc caggtccgcg tgtcccggac gtggcagggt gatgggatcg
     9541 ggggatttct ctaccgggtc cggggtcatc cggctctccc agttggcata gtcccggttc
     9601 cggaccagca tggctatggg agatcccagc gtgtaaccgt ggcgaacgcc gccaaggaac
     9661 tcgatctcgt ccttctctat cttctgcctt ccgccgcggc cgtaaccctt ctgcctgcgg
     9721 gccaggtcgc ggttcacgtc gtctgcgagc agtccgagcc ccgccggaac cccgtggacc
     9781 agggccacct cggcgggacc gtgtgattca cccgccgttg agaagctgaa cttcaacgcc
     9841 gcacgtcctc tcttagatcg ctcaagatac cgggctaata taaaccaaca tcgggctcgc
     9901 gttcacccgc ctccgcgcat gaagggccgg ataccgccgt gaggtccagc taggggagcg
     9961 atccaccact gttgaagaga ccgccattac ctttgccggt agtggtcccg cctccgccac
    10021 cgttcccgcc gccattgccg ccggtggtgc gcgactggcc gtccgggatg ccatcgccgt
    10081 tggagtcgtc cgcgccgtcg agagtaccgt cgccgtcggt gtcggggttc tgcgggtcct
    10141 ggccgagcgc ctgctcccgg cggttgctca ggccgtcgtt gtcagagtcc tggaagccgc
    10201 gtgagctgcc gccgcctccg ccactggtgt tctccccgcc acccccggtc gtgctgccgc
    10261 cgctgttgcc tcctcccgac ccggtggtgt tttccggcgg ggtggagcct cctgagccac
    10321 ccgtagtctc ctgagtggga ttcgtgctct cggcgggggc gaagagttga cggaacgggt
    10381 ccttgctctc gtaggcggcg taggagtcgg cgtcccggct ctcgacctgg ggggccggag
    10441 agtcggagcc gctatcgttg gaattgtcgg gagcctgagc tacgacgttc gcctggttcg
    10501 cgacgcgctc ctcttcaggc ttgccgacga agatcccggc gacgacccag gagaagatca
    10561 ggagcgccag caccgcgaga accggcggta tgaccttgtt gtcgcgcagg aacgaatcaa
    10621 agaagccggg gccgatggcg aggatccgcc gcgcgatccc ggtgtcccgt atcctactga
    10681 gcactggtcg tctctccccc cgctccgggc gtagttgtct gcggagccgc gggggctacg
    10741 ggcgcggtcc catccggcac gttctccggc tggaagtaga cgtcggcctc tatctctacc
    10801 tgcaaggtcg ggtccgtccc cgcctgcgcc gtggtgccct ccgcctcgac cggttcatag
    10861 ctcactttct ttaccgtgac cagccgggcc aggttctccg tgcgaagcag gaagtcctgg
    10921 agctggtcgt atgtcccttc aaacgacatt gtaatgggtt ggaccgaaaa gtcaccgcct
    10981 ccgggagccg ggccgggcga tccgggcacg atggagatct gcgtgacacc cgcctggtcc
    11041 gctatctcct cgacctggac gacgagcgtc gggatctccg gctgcgtggg tatgcgtttg
    11101 gagagctcca gtagctgccg ctccacggtc ggcgcgtcgt tcttgatctt ctccaggtcc
    11161 gcgacttgct gctggagttg ctggagctgc gcttccttgt cggagcgctc ctgtaccgca
    11221 tcgtcgtagc gggtccaaag cgggctgagg agaaggaaat agtaaccgac gatcaacacc
    11281 acgattatga gcgcgcccag gatcgcgacg ttgcggtcgt tgcggttcac tagcgcctgc
    11341 cgtcctcacc gctggcctgg gaacccgtgg cgtcctgcgt ctgggtagtc gcctcggagc
    11401 ccccggagga gtcgctgccg ctgtcgagtg ggagttccgt acccttcgaa ccgacctccg
    11461 tgatcaactc ggccgccacc tcgaagttga tcgcgtcctg ggagaaggtg tcccggtcga
    11521 gctccgcgga gtctagctgc gagttggcga ggaagcgcag gttgttcatc tgcacgacga
    11581 agtccgcgac gttggtgtac tggggcaacg agacgcccgt gaacgtcacg gcgcccggcg
    11641 ggttcaacgg ctgctcggcc ggcgcgtcta tgttgatggg cgctgcctct gcggtcagcg
    11701 tatcgagggc ggtggacggc gggatcacga acgccagccc ctgcaggaag tcgttccagg
    11761 ggaagcgggt acggaagatt ccgtccgcga tgggcttctt ggcgtccaga cgcgcctgaa
    11821 ggtcgcggta cggcgccagc tcggccagcc tctggttctg ctgggctatc tgggtatcca
    11881 ggtccgctat cttgtcctcc tcgttgttca accgcacgag gaagaacagg taggtggcga
    11941 ccatgatcat gaccgacagc gccgccgcta tgagcagtat ccccgtaacc ccactccgaa
    12001 ggcgcccgcc gatatcccgg cggcgatcct cctgcgggag caggtttatc cgcctcatga
    12061 ctcctccatg gcgagcccca aggcgaccgc cagcaccggc tccatgaccc ggacctgttc
    12121 atcggagacg ttggagcggt tggcgacgat cttggagagc gggttgcccc ggagagtatg
    12181 gataccgagc aactcgctca ggtagtcgtc tatcccggcg accagcgcgc cctcgcccgt
    12241 tacgaagacc tgcgacaact cgcgcgcccc aggctgcgag taatgataat caatggaccg
    12301 ctgcacgtcc tccgccaaca ggccgaccgc gtcctccaaa ccccgccgca cgtcgtaatc
    12361 gagcgccggg tcccattgat actccggacc ctcgacgctt gtctctggtt cctcagccgc
    12421 tgtctcttcg gcgccgtccg gctcggggcc gttttcggtc gctcccgctt ccggttccca
    12481 gcccgcctcg ctccgctctt ctccgctctg ctccgcggcg tccggcggtg tcaccctctc
    12541 gctctccggg tctcgcagtt ccgcagtctc ctcgtccgcg aatgccgtgt ccctcgcgtc
    12601 cagctccgca gtgtcttctc cggtgctccg ggcgtcaaga ggagtctccc gggtgtcggg
    12661 ttctgaatgc ggaccttcct gggtctgctc cgcatccagg gcatagccca ggcgcaccct
    12721 cgggttcatg agatgcttct cggcctcttc gtccgggatg tccgcggcgt cggcgatagc
    12781 ctggatgaag tacagcgagc cgccgggcac gaactgggta agttcgggcg agcctccctg
    12841 gtacacgacc aggttcgtca actcggagcc gatgtccatg atcagcacgg cgccttccgc
    12901 atcgtatccg ccttgcggaa ggacggagcg ggtcagggcg agggccttta cgtccacgcc
    12961 gaccggccgc agaccgcccg cccgcgcggc ggaggtgtag cgagagatca tctccctctg
    13021 agccgctact atgaggatgc ggtcgaggtc cgagccctct gcggcggggc ccagcaccac
    13081 gtagtccatg accgcctcct caagcggcat tggaatgtgg tcctgggcct cgtaacctat
    13141 ggccgtcttc agatcctcgg gagacatacg cgggaagtca accagccgca ccacaacctt
    13201 ctggttggag agccccagga tcaccgactt ccccttgaac gagtgggagt cccagaactc
    13261 cttgatctcg gccgcgagca ggtcgtgatc cgcgacttct ccgtcggcta tagtgccggg
    13321 cggcagccga tggtagccga cgtgctggag ggtgtagccg ccggaccccc gcgagacctg
    13381 aaccgccttg agcgagccgc gatcgatatc caggccgatt gtctgtgtgc ggaagcgccc
    13441 cacccttctc tatgcccctc cggcaaagtc caggtagaag ctccacatct cccgtcccgc
    13501 gagcagcgcc accacacccc cgagagcgag aaagaccccg aatggcatcg ccgtcctccg
    13561 ccgcattttt ccagtcgaga tcaggacccc tccaacgaga gccccgacga gcgccccgat
    13621 gaatacggcg agagccgcgt atggcccgag aaacgctccg agcatccccc ccatcttgac
    13681 gtcgcccatc cccatccctc ccggatagac gagcgcaagt cccaggagtc ctccagcgac
    13741 cgcgacggcc gacacgagat acacccacca catcgcgggc gcggcgagcg tcgagagcag
    13801 taaaccggcc agcgccgccg gcccgacgat gacattcggc agcagccggt gctccaggtc
    13861 tatcccggcc agagccacca gcacggctat cagcacgagc gctccggcca actccgcacc
    13921 gatcccgaac cgccatgccg ccacgccaaa cagcacgccc gtcagcgcct cgacgagcgg
    13981 gtagcgcgca gatatgcgcg cgccgcaatt tctgcacttc cctcgcagca gcacatatga
    14041 gaggaccggg acgttgtcca gcggcgatat ctccgcgccg cacttcggac atctcgacga
    14101 cggccacacg attgactcgt gccggggtac gcggtgtatg acgacgttga ggaagctacc
    14161 caccagtagt ccaagcaggg ccgccaggac tgtgatcaca ccgaatcacc ctcggggatg
    14221 cggctcagcg actccaaccg cgcgttcgcc cggacggtcg ccttcctgcc gacgctgaca
    14281 ctgtggccga gcaccgcccg cgctccgatc cgggctccag aacccacgat tgcgcccgcc
    14341 gctatccagg cccccgcccc gacctccgag tcccggtcta tcacggctcc cggaccgaca
    14401 tagcaatgac tcccgactac cgcgtcggga tgaacgaccg cgctcgccgc cacgaccgtc
    14461 ccatcgccga ccaccgcgac ctccgacacg tacgccagcg gatgcagcgc cgtaacaaat
    14521 tgtcccccaa gcgcccggat agagttggcg atccgcaacc gggcctcgtt gtccgtgatc
    14581 gccactatga tgtgtacggg ctcgacagag agcatgctct tgagcatccc tatgtccccg
    14641 agaattggaa accctcggac cttatccgca agtacagagt gagcgtcatc gtagaacccc
    14701 atgacccatc tctcggaccc gctcgcgagc agtacgtcga gcgcgacccg cccgaacccg
    14761 ccagcgccga caatggcgac ccttagctcc tcttctattt cgacgcgctc cacctgaact
    14821 tccgggtcag tgacactggt agtgatttcg gggtctctta tagcgctctt ctcccatacg
    14881 ctagaacagg cgttggggcg cagtttagca ccgtcgctaa caccttgcgc ctatctctac
    14941 atcctgcgct gcttcggaga catcggcacc atctgttgta cctccataac actacaaatt
    15001 gtgcctcctc agacgcggaa tcgccctccc agagcattgc cgcgcaggaa attcgctgcc
    15061 ggcagagccc tgccaccggg agcctgaatc tccagcacct ccacaactcc cctgccgcat
    15121 ccgacaaaaa tacggccatt tgcagcataa atttctccag gtctcagaga gacctcggat
    15181 tcataggcga tccgagagcg caggatcctc acgggcccat cgtactcctc gtgaaaagtg
    15241 cgcgccccta tatatggtgc cagcgcgcgt acaatgttat gcacctcatc cgccccgatg
    15301 tcccacttta tgacccgatc ctcattcgaa agtttagcag catatgtagc tttaagacta
    15361 tcctgttcgg ttaaggtaag gcttccggtc tccagcagag acatactctc aactacagct
    15421 tcagatccga gacgcgccag cagttcggtg agttctccgc cggtcatgtc cgctgggatc
    15481 gcggtttcgc gctgcagtgc catcggtccg gtgtcgagtc cctcgtccat ctgcataatc
    15541 gtgacgccgg tacgctgttc cccggccatg atggcgcgtt ccactggggc ggctccccgg
    15601 taggcgggaa ggagcgaggc gtggacgttc caagcgccat gactcgcggc gtagagtgtg
    15661 tcgggacgca ggatctgtcc gtatgcggca acgatgagag cgtcgtggtc cgagatctcc
    15721 gcggcggcct ccgagatgcg ctccggctgt cgtagcggca agtctctcgc atgcgccaga
    15781 tcggcgacag gggtcgaggc tacgcgccgt cctctgccgc ggcgggcgtc gggttgcgag
    15841 atgaccagac cgacctcatg gtccgactcg agtaggccgc tgaggatagt agcggcgaac
    15901 tgcggggtcc cggcgaaagc cagcttcacg gcgcgggcgt tctactggga ggcgagaagc
    15961 cgttcgcgca gctctcgcat cgcggccttg cgcgactcgc ggtcggttcg gtccaggatc
    16021 aggacgccgt tcaaatggtc tatctcgtgc tgcatgacgc gggcgagcag tcccgccgcc
    16081 tcgatacgaa gcggctcgcc ggtaacgctc tgtcccgaca ccgagacgct cgctggccgc
    16141 tcgacctcga cgctaatacc cgggatcgag aggcaccctt cggcgtccgt gacggtatct
    16201 tccgaggagg actcgatctc cgggttcacg atcacgtact cctcgtcctc ggtgccggcc
    16261 acgaatatac gcttcagacg gcctacctgg ttggcggcga gcccgactcc ttcctgttcc
    16321 ctcatcgtgg agagcatctc gtcggccagc cgtgccagcg actcatcgaa gctcttaacc
    16381 ggcgcggcgc gcgacttcaa gatcggatct ccaaacgtcc tcatctcaag cgccatctct
    16441 tagacctcct cgggatcaat accaaccctt accttcatac cgccgcgtgt ccccgtagcc
    16501 aggcgcgccg ccagcgctcc gccccgcgct accggagccc gctcgcgccc gcgcaacagg
    16561 actctccaga ttgcgggaga gtctccagtc ctggacaccg gcaccggtcc cgtcatgcgc
    16621 actccggtct ccaggccgct acgcaacccg gattctaccg cacgccggac gacctcctcg
    16681 gccccctcaa gtgtcacatc cgccagatgc gcgaacggcg gataccccag tgagagcagc
    16741 cggggaagct ccgcggacgc gaatcccgga tagtctgcgc gcaccgctgc ccgcagggcg
    16801 taatgctccg ggactctggt ctgcacatac aggtactccg ccgcagcctc ggcagcgaaa
    16861 tacagccgtc tgaaagcgcg ctccacggcc ccgactccgc tcccaagaag aaggccgtca
    16921 ttgtcaggaa tcattactac gtcccagtcc cggcccagga tgtaccgggc cgtgccgaca
    16981 acaacgggag cgtcgctcct cctggactcg cgagccgtca ccaatccgac cggctctcca
    17041 agtgcctccg acagcacttc ccgcgcccgc tccactgcca gccccgttgg gctcatcctc
    17101 tccgagccgc atgcgctgca agtccgtggg gcttcctccg cgtatgcgca gcgcgggcac
    17161 acgagcctgc gcgactcatc gtatgggacc aacgggatat cgcacacggg gcagctacgg
    17221 acggctccac agctagtaca ggcgacggcg gtcgcgtatc cggtccgatt cacgaggacg
    17281 cctacccgct tcccatcctg gatatgatgg cggcaggcct cgatcagggt ggagctcaac
    17341 gcagctccgg agccgcgcat atccactatc cgcacagcgg gccagtcttc tggcagccgc
    17401 gcgggcaact cgcgcaccct atctttcgac gcgaagagcc tgagagaggg cgcgggagag
    17461 aggaacaaca cccctgcccc ctccagttca ccgcgtttca ggcagatctc ccggacatgc
    17521 agcggcagtc cttcgtatcc gggctcggcc cgatgggcct cgttcggttc gtccacgacg
    17581 cagatagcgc cgagacggcg catcggcagc agtgccgccg tccgggtccc gattagaacg
    17641 tccacctcgc ccttcgcggc gcacctgtag atcacacctc gattccgccc gacggcggaa
    17701 tggtatgcgg caaccgagta acctgtcctc aactccctcc gtagacatcc cgccagacgc
    17761 tccgccgttt ctcgctccgg gaccagcacc agcacctgac ggccctgctc cacagcggaa
    17821 cgggataacg cggcgacctc agagaaggta tctttcgacg ggactctgtg cagacaggcg
    17881 ccgccatcca ggactcggag catctcaccg gttgcaggat caccgccgga gtcctccgtg
    17941 ccacctctcg tcgcccatac ggcgctcggg ccatctcgct gctccagcct caccacaccg
    18001 cgccggacca ggctggttag cgtcacccgg ctggccccgg tctccgagag taaatcggcg
    18061 gcaggatact cggcgccgcg gtccatcagg gaaccgagca gttctttctg tttgggcgtc
    18121 catttgaaac ccgggttcgc gccggaaagt aaggttgccc attgggtctc ctccggcgcg
    18181 ggcagaacag ggacgagctc cagacgcttc tccctctccg ccgccacgag cccggcggct
    18241 cccagccgcc gccgggcgac cgcgcgccgg acgagagcgc cctctctcca cggccagccg
    18301 gctaaaggcc cggagaccct gtagctgccc gtatccatcc cgggaggaag ggccgcgcga
    18361 actacgacgg ccagcggcag cgcggtgttc tcagagatcc agccacagag gtctaccatc
    18421 tcctccctca gagacaggcc ttcggcgacg ctgtatatct cctccagttc gtgctccgcg
    18481 gtatactcaa agccgactac gactcctatc cgggagtgct gcttgagcgg cgcgacgacc
    18541 atcgatccca tcctgacctc tcgtgccagc gtagcgggga cgcggtagct aaggggcgga
    18601 agggcgtgcg tggggatgag cagacagaca cgagccgctg cgtcacggct ccgctgtccg
    18661 ctcacagaag cccaggttcg gccaattaga gtccggcggc ccgagccagg gcgtcggcgc
    18721 gcgaggtatc ttcccaggta aagccgggct cccgccggcc aaagtgcccg taggcggctg
    18781 tccgggcgaa gacgggcttg cggagatcca ggtcgcggat gatagcgccg ggacgcaggt
    18841 cgaaatgctc ctgcaccagg ccgctgatcg taaccgggtc cacgctggcc gtgccgaagg
    18901 tctcgaccat cacgctgacc gggtgcgcga cgccgatggc gtaggcgacc tgcaccatgc
    18961 accggcgcgc cagcccggcg gccaccacgt tcttggcgac ccagcgcgcg gcgtaggagc
    19021 cggaacggtc caccttcgtc gcgtccttgc cggagaacgc gccgccgcca tgcggagccg
    19081 cgccaccgta ggtatccacg atgatcttac gtccggtgag gccggtgtcg ccctggggac
    19141 cgccagtgac gaaccggccg gttgggttga tcaggatctc ggcgacactc tcatcgaaga
    19201 gatgatcgga catcacggga cgaataacgg tctcgatgag gtcgtcccgg atgtgatgct
    19261 ccatgccatc gcggtgttgg gcggagatga ggatcttctc gataccgacc ggcgtgtctc
    19321 cctcgtagcg caccgtcacc tggctcttgc cgtcaggacg caggtagtcg agctgcctgc
    19381 tcttgcggac ctcggagagg cggcgcgtca gggcgtgggc gagcgtgatc gggagcggca
    19441 taagctcgtc ggtctcctcg cacgcgtagc caaacatcat gccctggtcg ccggccccga
    19501 tctcgtcgat gttctcctcg gtctcgcgga cctccagggc cttatcaacg ccctgggcga
    19561 tgtcggcgga ctgcggatct atggagacca tcacgccgca cgtctcggcg tcgaagccga
    19621 acttggcgcg ggtgtaaccg atctcggcga ccttctggcg cacgagagtc gggatatcca
    19681 cataggtgct ggtcgtgatc tcgccggcga taatcgccag gcccgtcgta acgagcgtct
    19741 cgcacgccac ccggctcatc gggtcgtcgg cgagcgccgc gtcgagtatg gtgtcggaga
    19801 tctggtcagc tatcttgtcc gggtgtccct cggtgacgga ctccgaggta aagaggtgcg
    19861 aagccctcag tttctggtcg tccgccgcgt tccaggtagt cgtcccaacc gtcttttctg
    19921 ccatctaatc tcgcctctct cgttctatct cagccataat atctctcaga atggcgcgtg
    19981 cgatttcggt cttcgaggct cgcggaacga gacgttcgcc gtcccggctc accacgtaga
    20041 cctcgttctc ctcggacccg aagccgatac ccgaccgcga aatgtcgttc ccgacaacca
    20101 ggtccacgcc cttcttgctc aatttctccc gggcgtccgg cacggggtcg ccgtgcgttg
    20161 ccgcgaatcc taccataaag aggtccctgt tccgctcccg gaccgccgcc agtatgtcct
    20221 ccgtcgccac gaactccacg gagagcccct cgctgcggcg cacctttgcc tgcatgggcg
    20281 tagccggttt gaaatcagag accgccgccg ccatcaccag cacgtccgcg cccgcgacaa
    20341 ggtccatgac gctccggcgc atctcgtcca cggtctcgac cggatgccac tcgactcccg
    20401 gctcggcccg ctcgacgttg gccgctacca cggcgacctc cgccccgagc ctgcgcgcct
    20461 cccgggcgac cgcgagcccc atcttccccg aggaacggtt cccgacgaac cgcacgctgt
    20521 ctatcggctc ccgcgtgcct ccagcagtca cgaccacccg gagcccggag agtggaccgt
    20581 tcataccgcc gagtacggcc gccactacct cacctaccgc agccattttg ccgtccgttc
    20641 cctcaaggac cttgcagtcg tcggcgcgca gtagggcgag gttctccgcc acggctggat
    20701 gcctggccgt catgggatcc aggtccggca cgacgtatgc gggacgcagc ccagccacgt
    20761 aggcttcccc agccttgcct tccgcaagac cattcgccag acgggcgatg gtcccggccg
    20821 tcgccggtgc gaagatgacg gaagaataca tctcctcatc gtcagaagta gcggcgtacc
    20881 cgccaaacgc agcatgaccg acgaaattag tagttccggg ttcgaggaag atctcgacct
    20941 gttgaccgga cccgatcagc tcacggtaga tattgggggc agaaagcgcg ccggacgccg
    21001 gtccgaccga gagcaggatc aaagctcggt tctccggcgt ccgtttggtg gagcgtccgc
    21061 tctaggcggc gccctggtct ccctcgtcct ccggcgcgcc ggtatctacc tcggcggtgg
    21121 ccggagcgct ctcgccgctc ggcggatcca cgaagtcgct gccgtcagcg gcgccgacct
    21181 gctcctcggc ctgctcctcg gcttcgtcgg ccgggtcgcg gaacgtcacc tccagcttgt
    21241 cctggcgcaa ctcctcgatg gcgatgctca gcgggtgctg ggagcgggtg tgtacctgcg
    21301 gtcccggtat ccccagggcg tcgttcagcc cgagcgtcgc ggtgtactcg ttgatctggc
    21361 gagcccgctt ggcgctcgaa accaccagac cgtaacggga gtctaccttc tcaagcaact
    21421 cgtctatctt aagttcgttc agcatcttcg cctcctacga tatttcgcat aagccctatc
    21481 atatcttctc tggcctgttt gcggtccgcg ttgatcacct cgtggtcaaa ctcctcgcgg
    21541 gccgcgacct ccttgagggc cgtcgtcatc ctactctcaa cggcggccga tgtctcggtg
    21601 gcgcgtcctt ccaatcgccg cctcgtctcc tccagggacg gagcccggac gaaaaccatc
    21661 accgcatctg ggcgtttctc gcgcaccttc cttgcgccct gaagctctat ctccaggatc
    21721 accgagagtc ccgcgtccat taactcgtct acccgatgct ccggcgtgcc gtagaggttc
    21781 ccggagtact ccgcccactc caggaaatac ccggcctcta tccagcgctc gaactcctcg
    21841 cgggagagaa agatgtagtg ttggccgttc acctcaccgg gccgcggctt gcgagtggtc
    21901 gcggagaccg agtaggcaag ctccggcacg gtatccaggg cttgacggat cagggtagac
    21961 ttcccggccc ccgagggacc ggagacgacg atcagttttc ctcgacgcaa cccagagccc
    22021 tgatcaaacg gtcccgctgc ccaaaggtga gcccgacaag ggtcttcgac cggctgacgc
    22081 cggcctccct caatatgcgg gtcactttta tccgtcccat accccgtacc gacaggagca
    22141 tctcctcgac tttcgcgccc ttgagctcct ccgaagggtc catgagcaga tcatagatgt
    22201 tcaactctcc gctcttgagg tcccgcttgg tcgcggcacg ggtgaaccgt acccgattgg
    22261 cctcttcgag cgcgcgacgg cgttcctgcg atgtcctctg tggagccctc ctcaacatcg
    22321 gctacaaagt atatcattgg tccttctctg ccgtcaccag ccagaattct ccgtccatgt
    22381 attctccatc caagtatctg cagagccagg acattaaccc cgtgaggccc tacgttacct
    22441 gcggcctcgt ctcgcccagc ctcgaccagg aagcgggccg cgtcgaggct gcgtctggtg
    22501 cccggcgcga gcgcgaagcc ggtgagtacg acgtcgcgcc gcggcggcat tcgggtcagc
    22561 cgcgagtgcc ccttcgagct acgccgcatc aggcggtccg aaccaggtgg ttagcgcgtc
    22621 gcgcaatccg gtatccgtgc cgtcccccac ccaggcgacg tatccatcgg gtctgatcag
    22681 cacggcggcg ggggctgcta cttgacccaa aaccggaagc tcccacgtac caacgtattt
    22741 ggcgtcgatc agctgaactc gatccgccca tggcgtgatg tcgaagccgc cgggctcacc
    22801 gaggttgagt agcaccggcc gggcatcgtg cagcagggtg aagacccgca gcgggccgcc
    22861 cgtggtgacc agatcgagat ccggcatgcg gcgtccgagc agcgggtgtc cctcacccag
    22921 gtcgtaatga atgtccagac cggacatctc ggcggcgaac cgcttgcgcg gctcgtccat
    22981 gctcagcagc tcggacacgg tgtcgcgcaa cgcctcgatg cgatcgtcgg cgcgcatgag
    23041 cgcgacttgc gccatcgtgt tgcgcatcac gcgggcaccg accgggtggc gctcggcgtg
    23101 gtaagtgtcg aggaggctgt ccggcgacgt cttcttgacc acctgggcca gcttccatcc
    23161 caggttcacc gcgtcttgca cgccggtgtt gaggccctgc ccacccaccg gggaatgcac
    23221 gtgcgccgcg tcgccggcca gcaggacccg tctgtcgcgg taggccgcgg cctgccgggt
    23281 catgtcggtg aacctggaga tccaggtggg actgtggatc ccgtagtccg tcccgtaggc
    23341 ggcgatgagg ccctcgctga gatcgcgcag ggtgggctcg ctcgtgccga tctcggactc
    23401 ggtcagcatg accccgaccg ttcccccctt ctcgtagacc acctcgccgt ccttgatctc
    23461 gtactcgacc ttgcccaggg catgcatgcc cgagggagtg cggcggatgc ccagttccgg
    23521 ctcctcggcc atctcgacct gggcgatcag ggagctcgtc gtcgggtccc agccggggaa
    23581 gtcgatgctc gctgccttac ggaccagact gcggcctccg tcacacccga cgagatagtg
    23641 cgcccgcagc gcccggccgt cggacagctg gacgtcgacg ccggtgtcat cctgcgcgaa
    23701 accggtgacc tcggtgccgt agtggatcgg caccttcaac tcggcgaccc agccggccag
    23761 aatgcgctcg atgtggttct gccacagccc gagtccgtac gggtggcggg tgggaaagtc
    23821 gctgatgtcc aaacggacgc tggcgaaccc cgcgacctga gccacctgtc cctccgaaag
    23881 gaaccgatcg gcgatcccgc gctgatctag gatctcgatc gttcgtgcgt gcagaccgcc
    23941 cgcgcgcgag ccggggagct cctggctggc gcgccgctcg acaatggcaa cgtcgacccc
    24001 cgccaacgcc aactcgcccg ccaacatcag ccccgtcgga cctcctccgg cgatcaccac
    24061 cgcatgcccg gtcatagcca cactcccttc cgcatcacgc cggtcgcgat cggcgcatca
    24121 gaacgttcct cggccgccgc ggcgaccgtg tcgaactgcg cggcgaccaa cttggggccg
    24181 cgccgcaaag tccggcggcg cgcacacccg cgcctccgcg ccgagtgccc gcgaccgcac
    24241 cgcgagtccc accatcggtt cgacgtcccc gcgcgaccca tacgccgaca agaacacacg
    24301 catttcgcta ctcccatttc cctgggcttg gaccggcatt atgcggcacg accggggtct
    24361 tgacgcaagc cccccggtgc cctatatact gagggtggaa aggactggtc agacctactc
    24421 ataaaatccg cggctccctc gcgccgcgcc gcggacgatg tcataagtta cgccctcgac
    24481 aagaaccgaa aatcggcgac tgagcccgcg cttcacgaat catgcgggtc atccgtcacc
    24541 ttcatgccga ctcaccgcgt cctactggag atcaggctcc gagtatcatc gaaatcccaa
    24601 cgagacccgc cccctcgcgg caccccggtg tcacagggct tatgtcctag cttgatttat
    24661 ctgggccacg aatggcgtta tcgcggcatc ttggccgaaa gaggtgatct gcaatgaggc
    24721 gtttgccgta tttgtacgtt atctcagggt gccggcaagg tcagaggcgg caagacccat
    24781 ttctttcaag taagcggaga actctccgag gatctcgctc ggctcccgca cgaagctcgc
    24841 ggtgccgatt tgcacggcgg tcgctccggc gagcatgaac tccgcgacgt ccgtcccgct
    24901 ggccgctccg ccgctcccga tgaccggcac cttgacggcg cgcgagacgt catagaccgc
    24961 ccggagagcg accggtttta tggcgggacc ggagagccca ccccgtacca ggatcttccg
    25021 ggcccgggaa tctatggtca gcgccggaag ggtgttgatc accgtcagcg cgtcggcccc
    25081 ggcggcctca gcggcgcggg cgttctcgac cacgccctcg ttggttagtt tggcgaagac
    25141 caacttgtcc tggacaaccg ggcggcacgc tgcgaccacc tcctccacgg aagccggacc
    25201 ggcgcagaac gtaagcccgc cgtgctcgac gttcgggcaa gagaggttta gctctatacc
    25261 tgagatccgg gcgtcaccgg agatccgctc gcagagagcc gcgaactcgt ccaccgtgtc
    25321 ccccgcaacg gagacgaaga gcggcactcc gacatcaaaa gcatcgagca cttcgaggaa
    25381 accctcgacg ccggggttct ggagtccgat ggagttgacc atcccggcgg gcgtctcggc
    25441 gatgcgcggc ggcgggttgc cggccctcgg tacaggcgtc gtagtcttcg ggagcatagc
    25501 gccatagacg cccagcacct cgccaagcgc ctcccttgcc agcgtcccag aggccgggat
    25561 gagtggcgtt gcgagttgca ggccgcaaag ttccactgcg agatccaccc cggtcgcctg
    25621 ctcgcgcatc accacgccag gacctccgcg tcgaagacag gtccctcgac gcacgacctg
    25681 acgtagccgc cgcctcgcac cgggacaaca cagccgttac acgagccgtt gccacagccc
    25741 atccgctcct ccacggagag ttgacagacg ctgtctcgtc cagccgcttt cacggccgcc
    25801 agcatcgggt tcggtccgca ggcgtagaca gcatcgtagc cttcgaggtc cggcacgacg
    25861 tccagcacgg tcccgcgcgt acccgaggac ccatcaactg tcgcaacctt tgcgctggga
    25921 aactcgcctg cgactggtac gaacctcgct tccgcgaagc ccagaaagat atcgtgaggg
    25981 atattcagag ctgtgaggcg gcgggaaagt atcttgagcg gggccacccc gattccgcct
    26041 cccagaagag ccgaccgctc cgcggttggc tggatcccga accctctacc gagcggcgcg
    26101 ctgacctcta tcctctccgg cgcccgatcc aggagcgccg tcccccttcc ccgcacctcg
    26161 aagagcaggc tcgccacccc gccctcgtag tcgtagaacg agaacggccg cgccagaaaa
    26221 ggatcgaacg taatgggata tccggcggcg cgagccatga cgaactgccc cggttccacg
    26281 gagccttcgg gccatcggta gctcaatagc gtgtagccgc caatctcccg gcgctcttcg
    26341 atctctacgg aatagaacct cacgtcttgg tggcgtagag gccctggagg ggattaactc
    26401 gccgcatgcc accccggatc ttcgactcga tgccctgcac cgcggcagca gcggcggcga
    26461 gagtcgttat gcaaggcacg ccgtgcagca gggccttgcg acggatcaga tatccgtccg
    26521 tgcgcgctcc acggccccag ggcgtgttca ggatcaggtc tacgccgccg tcctcgatca
    26581 gcccgagaac gtcttccccc agctccccga tcttcggcac caccgtaacg ggcaaaccat
    26641 tgttctggag tacctccgcc gtcccctcgc tcgccgcgat ctcgaacccg aggtccgcga
    26701 acgcccgcgc cagcaacacg accgaccgct tgtcccggtt ggagacggag atgtacaccc
    26761 gccccgccac cgggagcgtc agccccaccg ccgttagcgc cttggcgaac gccccgccaa
    26821 aggtagcgtc tatccccatc gcctcgcccg tcga
//
LOCUS       DASSMB010000016        31271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_510032, whole genome shotgun sequence.
ACCESSION   DASSMB010000016 DASSMB010000000
VERSION     DASSMB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 31271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31271
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510032"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VFJ72_01225"
     CDS             complement(<1..874)
                     /locus_tag="VFJ72_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HET7478113.1"
                     /translation="MADKTLMESMRRSKAGAYAWSKHMKSMRLWLLLLVPLLLLYPLF
                     LFVFVFSGRLSPVGTTVGTFMIVGICWAMTKLMESRDWRWEEGARGEFLVGEELEKLY
                     KEGFHVFHDYFALDRGNVDHFVVGPPGVFAIETKAWKGEITVTDDQLRVDGQPRSKDP
                     IAQVKGEAKDVRRLIADACGATYWVNPIVCFSTGELRHYGPVRGVEVTSVGSLRNAIM
                     RHPRRRSSANLLSASEVGAVSRYLQKHLGVMPASAPGSPPLPPGRLRRMMRSEWTYVT
                     LYVLYMLVLSLIFAG"
     gene            complement(911..1510)
                     /locus_tag="VFJ72_01230"
     CDS             complement(911..1510)
                     /locus_tag="VFJ72_01230"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7478114.1"
                     /translation="MIIDPRDLDPQSVYKLLIGSVVPRPIAWTSTVSRDGVRNLAPFS
                     FFTVASRNPPMLCISIGPRTSDDQFTKDTLDNIEETGEFVINIVSLPLSNTMHESSKT
                     HPPEADEFEKAGLTPAPCEVVKAPRVKEAGVSMECVLDRIIPLGSDHLVIGKMVRFHV
                     RDELYQNGRIDVDGLQPLGRLAGNYTKVETIFELPSEDL"
     gene            complement(1517..1918)
                     /locus_tag="VFJ72_01235"
     CDS             complement(1517..1918)
                     /locus_tag="VFJ72_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7478115.1"
                     /translation="MEKLRDAGLRITPQRRAVWSAFEAGAGEHLTADEVFGRARDELP
                     ELSRATVYNTLSVLVDVGLLRMIESRGAVLYDSDPDPTHHHFRCRSCDRLYDVHVEGV
                     EGLKISGDRKFMVDWKNVLLGGLCPECLPAK"
     gene            complement(2086..2973)
                     /locus_tag="VFJ72_01240"
     CDS             complement(2086..2973)
                     /locus_tag="VFJ72_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HET7478116.1"
                     /translation="MFLRIDKLQVELPRPSEADPAAAATVQELMGGKFGEMSTLMNYT
                     YQSFNMRGKSRIRPYYDLVANIAAEEMGHIELVANTINLLLDKTVENADGSLGTAPLG
                     MGLGAGYPDHFLNTGLGTMAAGAGGAKWSGDYVFSTGNLKLDLLHNFFLESGARMGKI
                     RVYETTDNPVAREMVGYLIVRGGVHQEAYAKALSDLSGVDVTKLLPVPDIQSEKYPHA
                     RKFMDKGYHRFLYRFSPDDYRQMGEIWNGPQADTGEEREVVDGIPEGGEVPDLTPVPE
                     MFAPTVSPEEIDEIARKLG"
     gene            complement(3239..4837)
                     /gene="pckA"
                     /locus_tag="VFJ72_01245"
     CDS             complement(3239..4837)
                     /gene="pckA"
                     /locus_tag="VFJ72_01245"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET7478117.1"
                     /translation="MDVAERKTTLERFGLTHLGAVHENLPPARLVEAAVRRREGMISS
                     SGALLAKTGKRTGRSPKDRFIVENEMTRDTVAWGAVNKPFSPEAFDAILEKASAYMEN
                     LDEVYVVDAYAGADPRYRLNVQVVAEFAWHALFARQLFRRPDRGELRSFEPEWTVISV
                     PGYLTDPEEDGTESETFVGLDFERKLVLICGTRYAGEIKKSIFSVLNFVLPTEYDVFP
                     MHCSANVGPNDDVALFFGLSGTGKTTLSADSERRLIGDDEHGWSDEGVFNFEGGCYAK
                     TIDLSPDKEPQIYDAIRFGAVLENVGMDRKTRQEDYTDRALTENTRAAYPLEHIQGAV
                     PEGMAGHPSAVLFLTADAFGVLPPISALTPEQAAYYFLSGYTAKLAGTEADMETDVEA
                     TFSTCFGAPFLPLPATTYSTMLSDRLREHGTRCYLINTGWSGGPYGVGSRVDIAATRE
                     MVRAVIGGKLDDAVTEKDPFFGLNVPKEVPGVPTEILDPRETWSDKEAYDEQAKKLSD
                     LFRENFKKFEAEVSDEVREAGPHF"
     gene            complement(5087..5557)
                     /locus_tag="VFJ72_01250"
     CDS             complement(5087..5557)
                     /locus_tag="VFJ72_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HET7478118.1"
                     /translation="MLSVTFHAAHAILAGVWLGGVVFTTAVVSPALKAMKWEEHERVS
                     VRSRIGKQYARVGSVNLILLVIFALLDGLSSGFGVVRYVEYLLLAVLLGLVAAHGAYF
                     GRKLAGLAEAERQAGSGEEARALAMRRRDLQRLSLRVSWADMIVSLVVAALAVI"
     gene            complement(5766..6194)
                     /locus_tag="VFJ72_01255"
     CDS             complement(5766..6194)
                     /locus_tag="VFJ72_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7478119.1"
                     /translation="MRLELSSEGRYALRALLYLAWTGERVTADRISADAHIPRRLLAR
                     ILANLSRAGLVESEQGRGGGSRLTRPARDISLREAVEAVEGPFAVTRCIMQNRNCGEG
                     APCALHEALEEGQQAILDYLDAQTLEDFRTQNPSGIAFEA"
     gene            6376..6771
                     /locus_tag="VFJ72_01260"
     CDS             6376..6771
                     /locus_tag="VFJ72_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7478120.1"
                     /translation="MTTRPHPESARPMHGALQRFDLEGEVSRLRGEKEWREGGRNAIT
                     LRKGGGLSVVLLVMKAGDRLEEHVAPGPISLSVHEGRIRFSAAGETVEAGTGTVLTCD
                     AGVRHSVEALGDAVCLLNVTAGGEVWDPE"
     gene            6785..7126
                     /locus_tag="VFJ72_01265"
     CDS             6785..7126
                     /locus_tag="VFJ72_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7478121.1"
                     /translation="MDLTKTAARAEDRNGVVWALEESGDLNANLVRFGTGRGVGEHVN
                     DEVDVLFLGVSGAGFVIVDNEKHALSAGKLVFAPRGTRRSTIGASDEFSYLTVHRRRG
                     PLRISERISEK"
     gene            7370..8479
                     /locus_tag="VFJ72_01270"
     CDS             7370..8479
                     /locus_tag="VFJ72_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7478122.1"
                     /translation="MDVTAAVVREKEQPFVIEELALEEPRADEVLVRIVGAGVCHTDL
                     IVRDQWYPVPLPAVLGHEGAGVVEKVGDGVTKVQPGDHVVLSYRACGKCINCLRGKPP
                     YCLKLYEENFGGARTDGSNALSKGSETVHGHFFGQSSFATYSLAAERNVVKVRDDVPL
                     ELLGPLGCGIQTGAGGVLNSLHPEAGSSIAVFGTGSVGMSAIMAARVAGCATIIGVDV
                     KPNRLELAKELGATHTINAAESGNVVEEIQGITGGGADYTVETTASPQVFRQAVDSLQ
                     LLGVCGLIGAAALGTEATLDMNNILFGRTVRGIIEGDSIPDIFIPRLIELYAQGRFPF
                     DRLIRFYELDEINQAAEDSESGEVFKPVLRMPQGA"
     gene            complement(8861..9679)
                     /locus_tag="VFJ72_01275"
     CDS             complement(8861..9679)
                     /locus_tag="VFJ72_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET7478123.1"
                     /translation="MGFLKRGWVRIFFSGFVLLYLVERVLVGTGNPNYIPSVILLGAF
                     LVPVTFVTYLYERLPKWEISLPSLAVCFLWGGVLGTVVAGTLEYDVARTLGFVPKLFI
                     GFIEEGAKLIVPLVFYFMGRYRSEAAGIVLGVATAMGFAALETMGYGFVTLLQSKGNL
                     GALDEVLLLRGLTSPAGHAAWTGIVCAVLWRERVKAGHARLDRRVVGAFLTAVILHAL
                     WDTFAGVRSATLAGYLGVEFLSLLVALVSLTLLIRRVREARRADAAEPRVAQPG"
     gene            complement(9754..10815)
                     /locus_tag="VFJ72_01280"
     CDS             complement(9754..10815)
                     /locus_tag="VFJ72_01280"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7478124.1"
                     /translation="MRLSGEMRRRFARGVLYVASIGLALHLMLPQIPGLRRSVDLMME
                     AAPALILLAFLAELLSELCYADLLGRSVMMSSGLGPYLRYRRHRGIGLWFTLRLAVTG
                     YGAAHVLPGGGATAATVTYAALRSRGFERKKIALAVAAVSALVYGALGAIFAGSLLYM
                     VLNESLGVAETSVALVGIIFVVVAGAGSYEAYRNPALTRRVLARILLLGGRLFRREWS
                     RRTVESWAATVVSTVRDEIKAARRQYISRARSTFGLAGLALGYWVFDALCLVVMFHAL
                     GVPAGTVELLVAYGIATAAGTLPLTPGGIGVFEATMIATLALLGVGSEAAIPVLGYRL
                     FNFWLPIPLAAVFYPTLRI"
     gene            complement(10969..11733)
                     /locus_tag="VFJ72_01285"
     CDS             complement(10969..11733)
                     /locus_tag="VFJ72_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET7478125.1"
                     /translation="MLRTALGGPWTEQLPIWCWVIEHPEGVILVDTGANTRMLEPGYF
                     TRWNPYFRRNVRVSVRPEEEIGPRLHSLGIEPGEVRRVVLTHLHLDHAGGLHHFPNAE
                     ILVSREEHRIATGPLGKAAGYLPHRWPSWFAPRLVDLELEPFGPFPRSLALTQAGDVW
                     LVGTEGHSAGHVSVVVLEKGRSLFLAGDASYTEELMLEQAIDGVSPSGNAARRTLGRI
                     LRYAEENQTVYLPGHDPASAERLAGRRTAQAETAVS"
     gene            11993..12457
                     /locus_tag="VFJ72_01290"
     CDS             11993..12457
                     /locus_tag="VFJ72_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HET7478126.1"
                     /translation="MNEIEELRYLVLAAQREGNRMLTQRLRPLGLTASQAEVLRVLQD
                     HQPLSLIELGGLLVCETGSPSRLVGGLVEAGLVERTLSEADKRMITLSLTREGEQTAS
                     EVAEVEAALYRTITEALNSEEISGVLAALRRLVAGRPAGEALSRRSRLSRLP"
     gene            complement(12557..13933)
                     /locus_tag="VFJ72_01295"
     CDS             complement(12557..13933)
                     /locus_tag="VFJ72_01295"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET7478127.1"
                     /translation="MATTKIQEERIQDLNEEDARDGDYVLYWMQSSQRAEYNHALEYA
                     VRQANDFGQSLLVVFGLTDDYPEANLRHYAFMLEGLQDVEDALNKRGIKLAVRKGSPD
                     EVALEAGKDASLIVTDRGYLRPQKEWRKKVANEAGCRVVQVETDVVVPVELASGKQEH
                     AARTLRPRIHEYLEDFLVELKPTKPEKQSLNMDAAGLDLSDIEKVLDGMDLDRSVGVL
                     SHLYRGGTSEAKKVFRRFLKKSFQDYEANRNQPQTDFVSHMSKYLHFGHVSPVWLALE
                     AQESNADKKNIDSFVEEVIVRRELSMNFVHYTPDYDSYSCLPGWAKKTLEEHKKDDRE
                     YTYTAKQLESAGTHDEYWNAAMREMIHTGYMHNYMRMYWGKKILEWSNTPEHAYRTTL
                     YLNNKYFLDGRDPNSFANVAWVFGQHDRGWTERAVYGKVRYMSAGGLERKSKPDQYVE
                     KVEERVRG"
     gene            complement(14027..15943)
                     /locus_tag="VFJ72_01300"
     CDS             complement(14027..15943)
                     /locus_tag="VFJ72_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7478128.1"
                     /translation="MVGAFQKTIRELLSGRDWIYLLSLLVPLTVYNLTLKAMSIAIKD
                     DAPGLWGSLDLMRSDILFNVGYGILWVGLFAVARRGALRKVAVVFFHVISLLIVTVST
                     VAYQYFEGTGSTLDYSVITFYLSTLGEVKEIIASEAAWYVWLGLILALLYAALGPWAI
                     TRLAARRSHEGTARTSRLEAAGLCLIAVGIASLSMVPGAENSDRSFSLSPLTNVLITG
                     IAAPGADELEVEKAGASVSVPLTDVKLKETPRTEKRNVVLIHLESTRERSVTPYNPDL
                     QTTPFLDELSKKSLLVERAYTTTPHTSKALTSINCGIYPDPSTDIHEAEPGGVPVKCL
                     PELLKEQGYNTVMFQSAIATFENRPQLAKNFGYEDFVGLKEMSKKGFQRSGYLGYEDD
                     ILLKPSEEWLTSHKDRPFMASYIGITPHHEYLAPTRYGRVPLAKDDVLNRYLNAVRYD
                     DFWIKNLIEQYKKLGLYKNTIFIIYGDHGEAFGEHGVKGHDGVVYEEGLRVPLIIHDP
                     QRWQNGAKISADAPIHHIDLPPTILDMLHYRVVNGDYPGASALKSPEGRTLYFNCRPD
                     LQCMASIKGYQKYIYYYGKQPDEFYDLKNDPLEKHNIIGQIPPAELDQRRNDLLEWRS
                     HAAAEFEKAPSKSK"
     gene            complement(16017..16433)
                     /gene="msrB"
                     /locus_tag="VFJ72_01305"
     CDS             complement(16017..16433)
                     /gene="msrB"
                     /locus_tag="VFJ72_01305"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET7478129.1"
                     /translation="MTDTPRKIQKTEEEWQDQLTSEQYRVTRMKGTERPFTGEYNDNK
                     ADGTYLCVACGTPLFSSDTKFESGTGWPSFWAPVDEGNVETESDRSFFMRRTEVHCAT
                     CGAHQGHVFDDGPAPTGLRYCINSAALRFEPESKPD"
     gene            complement(16462..17610)
                     /locus_tag="VFJ72_01310"
     CDS             complement(16462..17610)
                     /locus_tag="VFJ72_01310"
                     /inference="COORDINATES: protein motif:HMM:NF004811.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase"
                     /protein_id="HET7478130.1"
                     /translation="MSLRGRAAIVGVAESDLGEVGPGLTPLDLIGQATLRALDDAGLE
                     KSDVDGLFSASAYYHMPTLSAGEYLGIRPRYTDATSIGGSSFVSHLFHAAAAIDAGLC
                     EVALITYGSTQRSGGGRLVSGSETLPYEAPYNPRYPVSMYALAASRHMHEYGTTREQL
                     AEVAVAAREWAKLNPKAFMRDDLTVEDVLNSRMVSAPLTVRDCCLVTDGGCAAIVTSA
                     ERARDLKKTPIYLLGAGEAHWHRHISQMPDLTTSAAAESGPRAFGMAGLGPADVDVAM
                     LYDAFTINTVMFLEDLGFCRKGEGGAFVSGGRIAPGGELAVNTSGGGLSYNHPGMYGL
                     LLIVEAVRQLRGECGMRQVEDANVALVHGNGGVLSSQVTAVLGTERAL"
     gene            complement(17607..18002)
                     /locus_tag="VFJ72_01315"
     CDS             complement(17607..18002)
                     /locus_tag="VFJ72_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HET7478131.1"
                     /translation="MNRKNEPAPAETYHRYLEEGKLGFQLCRGCGAAVFYPRVLCPAC
                     GSGTLEWRSSSGRGTVYATTAVHRRDAEPYNVALVDLDEGFRMMSRVEGVSAKEVGIG
                     ARVALRVRREGDEPVAVFVPDAELEDGAG"
     gene            18162..19805
                     /locus_tag="VFJ72_01320"
     CDS             18162..19805
                     /locus_tag="VFJ72_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7478132.1"
                     /translation="MATGTTEARRGPEVHHLKRDVSLIGLLFVSLGSIIGSGWLFGAL
                     FAAQIAGPAALLAWIGGGIGMLLLALIHAELGGMYPVAGGSARYPHFSYGSLVGFAIG
                     WITWVGAVTVAPIEVEAALQYAANYFPWLTSTAGGVTVLTGPGIAVAAVLMLLFTIVN
                     LFGVKLLAESNNVIMIWKIAIPFLAIIVIMLLAFHTSNFTAKGGFMPFGVQSILSAIS
                     TGGIIFAYQGFEQAIQLGGETNNPQRNIPIAVIGAMLVGVVMYILLQVAFLGALDPKN
                     LSEGWTKLSFEGLAGPFAGLATAIGAGWLATLLYIDAAVSPGGTGLIYTGSSSRLSFA
                     MARNHYIPAFFGRLSERGVPTISVIFSFIVGMIMFLPFPGWQELVGFITSATVMGYAT
                     APLAFGALRRQEPDHPRPFNLPGGAVLAPLAFVIANLIIYWTGWPVLWKLFVAIALGF
                     VLLAIAQATSSSDRTPTLDWRSASWLWPYLAGMAIISYLGAFDGIKVIPFGWDIVVMT
                     VFSLAIYFFAMSVRLHPDEVRRHVADAREEAAVEEELAV"
     gene            complement(19870..20400)
                     /locus_tag="VFJ72_01325"
     CDS             complement(19870..20400)
                     /locus_tag="VFJ72_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET7478133.1"
                     /translation="MKREINGVTIECVRGDIAAQRDVTAVVNAANAGLMPGGGVAGAI
                     HRAAGPGLAEECRPLAPIRPGEAVITGGHDLPNRHVIHTLGPVYGQDRPEAELLADCY
                     RNSLTLADERGIESVAFPAISTGIFGYPVEEAAAVALKTVAETSGALENVRLVRFVLH
                     GERDLEVHEKALADLE"
     gene            complement(20397..21557)
                     /gene="nagA"
                     /locus_tag="VFJ72_01330"
     CDS             complement(20397..21557)
                     /gene="nagA"
                     /locus_tag="VFJ72_01330"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET7478134.1"
                     /translation="MKLVALRGRVVTDYEVWPEATVLIEGGSIRDIGRDGSLAEEADE
                     VHDYPDSLIVPGFVDLQVNGSFGVDVATEPGRLDELSEALLQTGTTSYLPTVISSPES
                     LYERALPELAARTGDPASGAEVLGIHLEGPFLNLEKRGAHAAAHLNPPNVALLERLLD
                     LGPVRMLTLAPELPGAGELMERARDRGTAVSAGHSDASFVLAYEAFDHQVSGVTHLFN
                     AMSPLHHREPGIPGAAFAHPLVVCGLIPDGLHVHPEVVGLAFRMLGPNRIYLTTDAIS
                     AAGMGAGEYRLATRTVYLESGVPRLGSGAIAGSILTMDRAFQNILAFTGCTIPEAVRM
                     ASTTPARLIGEGKRKGRLTPGYDADVTVLAPDLSVEAVWRCGRSVFSVEATG"
     gene            complement(21582..22925)
                     /locus_tag="VFJ72_01335"
     CDS             complement(21582..22925)
                     /locus_tag="VFJ72_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478135.1"
                     /translation="MTTGFRASRLAVWSWVLYDFANTIFSISILSFFFPLWVDEEIGN
                     GGWLVNSAVAVSALLVLCTAPVLGAMADFRQRRVPYLVVLTVISVFMTAGLGVSGGVV
                     VGVALFVAADLTYQSALVFYNALLPVVAAGRGDGRISGYGTAAGYVGSIFALLVLTVF
                     VDQAGAVHSLMGPLGGWIEISGENSSNAFLPTAVLYLLFSLPVFFFVPDPAVRAPRPV
                     SLGIAYRDVVRTLKNLRRYAGVGTFIIATILYTDAANTAISNMALYGRKVFDMDSGEI
                     RNLLLFSTVFAVVGSTICGFATDRYGPKRTLLAVLGLWLVSIALAAAALGPWMLYLAG
                     PLVGIALGGTWTVSRVMLIALSPPEKLGEFFGLFSLAGKVSAVTGPALTAILLFVLDG
                     LGGLAYRISIASLSLIMALGVYFLLRVPDVRPGESGAGYEEDAVREQAGTVDTSA"
     gene            complement(23020..23829)
                     /locus_tag="VFJ72_01340"
     CDS             complement(23020..23829)
                     /locus_tag="VFJ72_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET7478136.1"
                     /translation="MIEFLSGLPPVLQALVATTFTWGLTALGAALVFGTKDINRKLLD
                     SMLGFAAGVMIAASFWSLLAPAISLSKNGPLPMWIPPLAGFLAGGTFLRGLDLVLPHL
                     HPGHEMSDSEGIKTGWQRSVLLVSAITLHNIPEGLAVGVAFGAAASGIQDASLGAAVA
                     LAVGIGLQNFPEGTAVAMPLRREGMSRGKSFFYGQLSAVVEPVAGVLGAAAVVLAKPM
                     LPYALAFAAGAMIFVVAEELVPEAKRGSPDIAAMSVMVGFAVMMTLDVALG"
     gene            complement(23973..24584)
                     /locus_tag="VFJ72_01345"
     CDS             complement(23973..24584)
                     /locus_tag="VFJ72_01345"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7478137.1"
                     /translation="MAVLALALYALYLTLAVGGRMLLQLRRTGSTGLRGLGGRPGSAG
                     WLSGLLFVFAFGLGLLAPVLDLAGALVPLFPPALFSGLALYLVGLSGTLTAQLMMGDS
                     WRIGVDEREKTRLVTGGPFAVVRNPIFAAVIPTSLGLALLVPNLAALAGFAALFVALQ
                     MQVRLVEEPYLLRTHGDEYARYASRVGRFVPGIGRLRPPAASR"
     gene            complement(24584..25897)
                     /locus_tag="VFJ72_01350"
     CDS             complement(24584..25897)
                     /locus_tag="VFJ72_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7478138.1"
                     /translation="MADRSMEFIEGVEGVLPGETATARAAHQSLKGDRRGIRRLLPFL
                     GPAFIAAVAYVDPGNFATNISGGAQFGYLLLWVILTSNLMAMLIQSMSAKLGIATGKN
                     LPEVCRARFPKPVTFFLWVQAEVIAMATDLAEFIGAALALNLLFGIPLFPAGLITAVA
                     AFGILELQRKGFRPLEAVIAAMVGVIVVAFAFQMFYAEPRADRILAGLFTPQFAGTES
                     VLLAAGILGATVMPHVIYLHSALTQRRVVGRTEEERRKIFRFELVDVVIAMAIAGTIN
                     ASMLIMAAALFHSNGLTGVADIDRAFEQLRALEGNSASILFGVALLASGLSSSSVGTL
                     SGQVVMQGFINRQIPLLVRRLITMAPALVILAVGINPSRSLVISQVVLSFGIPFALIP
                     LLMFCRNRDLMGTLVNHRLTTAVAMVVVALIVSLNVFLLYQTFLG"
     gene            26017..26703
                     /locus_tag="VFJ72_01355"
     CDS             26017..26703
                     /locus_tag="VFJ72_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7478139.1"
                     /translation="MTNLTAPHSPSSSVGDYLKAVWELAGDTGEAASTKDVAARLSVS
                     PASVSNMFARLQEMGLVGYERYRGAMLTQSGRVEALRLVRRHRLIETFLLGHLGYSWE
                     EVHEEAERLEHAVSDGFTERLAEFLGHPGHDPHGDPIPTADGALEPDDSFPLGESEAK
                     ERVRIYKVSDEDARVLDYLAEHDLVPGRLLEVREIRALDGVVTVKDEDGRVHSLGEPL
                     ARSVFVRDEP"
     gene            26726..27556
                     /locus_tag="VFJ72_01360"
     CDS             26726..27556
                     /locus_tag="VFJ72_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HET7478140.1"
                     /translation="MDNPSPEPDYPRYRLGPGEQVSLAEIDPDQTEHYKRKKDVLKDL
                     EKQRRRIQDLQERLYAENEQGLLIVLQAMDTGGKDGTIKHVFSGVNPQGCRVSSFKTP
                     SAEEANHDFLWRYHKSAPAKGRIGIFNRSHYEDVLVVRVKDLVPEEVWRMRYEQINDF
                     ERNLTLNGITVLKFFLHISRAEQKKRLQARLDNPDKRWKFSSNDIKERAFWDDYQRAF
                     EGAIQKCSTEYAPWYVVPANKKWYRNLVIARTIADTLEAMDPQFPPAEEGLEKVRIPD
                     "
     gene            complement(27563..28036)
                     /locus_tag="VFJ72_01365"
     CDS             complement(27563..28036)
                     /locus_tag="VFJ72_01365"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7478141.1"
                     /translation="MRETRENGFLYFEDFEPGMVFDFGERTIDREEIVAFAREYDPQP
                     FHVDEESARDSAFGGLIASGWHTAAIFMRLYVDALLSRSASMGSPGVEELRWLKPVRP
                     GDTLRASLTVLDTAPSTRNPNRGTVHLYSEVRNQRGETVMTMKARGLFARRNGAE"
     gene            complement(28039..28824)
                     /locus_tag="VFJ72_01370"
     CDS             complement(28039..28824)
                     /locus_tag="VFJ72_01370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7478142.1"
                     /translation="MPENRVALVTGAGRGIGRTVAMTLAERGYRIAANDLRAPEETLR
                     EIEQIGGEALSVPGDVSDEASVRGMVEAMMSEFGRIDVLVNNAGISSITPAEETSLED
                     WNRTLSVNLTGPFLTCREFGKVVLERGEGSIVNISSVAGLLGIGDRAAYNASKHGLIG
                     LTRTLAAEWGGRGVRVNAVCPGWVKTEMDEEDQAGGGYTDADIEGRTPMGRFARPEDI
                     ARAVAFLADPEQSGYVNGHTLSVDGGWFGDGSWESLRRRKQRG"
     gene            complement(28838..29725)
                     /locus_tag="VFJ72_01375"
     CDS             complement(28838..29725)
                     /locus_tag="VFJ72_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7478143.1"
                     /translation="MTEPVRDRWAEWLLERRDGGDPVQQKASLEFLFPIRDRVLENAG
                     LSGGETLLDVGAGDGLIAFGALDIVGKNGRLIFGDISQDLLDHSKKLAGDTGVLDRCR
                     FLRASADDLGALEDESVDAVTTRSVLIYVEDKRRAFEEFYRVLKSGGRLSIAEPINSF
                     TYPEPEHLFMGYDVTPVQDLARKVRAVYERIQPPGDPMMDFDERDLADFADAAGFSEV
                     HLTLEISISRGNPSGILGGWDRFIRWTGNPKVPSLGEAMNEALIPHELERLESHLRPL
                     VERAERVSRDAFSYLWAVK"
     gene            complement(29729..30685)
                     /locus_tag="VFJ72_01380"
     CDS             complement(29729..30685)
                     /locus_tag="VFJ72_01380"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET7478144.1"
                     /translation="MPEKVLVTREIPAAGLRLLEDFDVTVLSEAPPERNELLEAARNS
                     SGVLSTVTEKMDAEFMDAAGDGLKVIANLAVGYDNVDLEAAKERSIVVTNTPGVLDET
                     TADVAFMLLLAAARRLGESERVLRAGKWEWWGPKLFMGPDVWGKKLGIIGMGRIGQAV
                     ARRARGFDMEVIYYNRSRNEEAEKELNARYVELDELLETADFVSVHTPLTDETRHLIG
                     AAELEKMKPEAVLVNTSRGPVVDEEALAEALAERRIFAAGLDVYEEEPKVHPKLLELE
                     NVVLAPHIGSGSIETRNKMATLAAENLVAVLRGEEPRTPVQS"
     gene            complement(30710..>31271)
                     /locus_tag="VFJ72_01385"
     CDS             complement(30710..>31271)
                     /locus_tag="VFJ72_01385"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563607.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET7478145.1"
                     /translation="RRGNHEVMVRGTFGNIRLRNKLAGGKEGGYTVHFPDGEETTIYE
                     ASLKYQEEGVPLLVLAGKEYGSGSSRDWAAKGSFLLGIKAVIAESFERIHRSNLIGMG
                     VVPLQYAEGESADSLGLTGKESFDIKGLADLEAGAELTVKATSDDGDETEFKVKARVD
                     SPVEVEYLRNGGILQTVLRQLLKEGQ"
BASE COUNT         5598 a        10050 c        10055 g         5568 t
ORIGIN      
        1 gaccagcgaa tattaaagaa agtaccaaca tgtagagcac gtatagcgtt acatatgtcc
       61 actccgacct catcatgcgt cgaagcctgc cagggggcag cggtggtgat ccgggagctg
      121 aggccggcat cactccaaga tgcttctgga ggtatcggga aacagcacct acttcagatg
      181 cggagagtaa gttggccgaa gaacgtcgcc gtggatggcg catgatcgca ttcctgagag
      241 agcctacgct cgtgacctcg actcctcgca cgggtccgta gtgacgaagt tctccggtac
      301 taaaacacac tataggattc acccaataag tagcgccaca ggcatcagct atgagtcgtc
      361 tgacatcctt cgcttctcct ttgacctgcg ctatgggatc cttgctcctc ggctgtccgt
      421 ccacccgaag ttgatcatct gtgaccgtga tctctccctt ccaggctttg gtctcgatag
      481 cgaagacgcc cggcgggcca acgacgaaat ggtcaacatt cccccggtcc aacgcaaagt
      541 agtcgtggaa cacgtggaag ccttctttat agagcttctc tagctcctct cccactagga
      601 attcacctct cgcgccttcc tcccagcgcc agtccctgga ttccatcagc ttcgtcatcg
      661 cccaacagat tccgactatc atgaaggtgc cgactgtggt tcctactgga ctcagtcttc
      721 ccgagaatac gaatacgaag agaaacagag gatatagaag caacaaagga acaagtagga
      781 gtaaccaaag cctcatactc ttcatgtgct tgctccaggc atacgcgcca gctttgcttc
      841 ttcgcataga ttccatgagg gtcttatcgg ccaaagccga ggaggccttt aatcactctg
      901 tagactgagg ttagagatcc tccgacggta gctcgaagat ggtctctact ttcgtgtagt
      961 ttccggcgag gcgtccgagt ggctggagcc cgtccacgtc tatgcggccg ttctggtaca
     1021 gctcgtcccg gacatggaag cggaccatct tgccgatcac gaggtggtcg gagccgagcg
     1081 ggatgatcct gtccaggaca cactccatgc tgacgcccgc ttcctttact cgcggcgcct
     1141 ttaccacctc gcagggagcc ggtgtgagtc ccgccttctc gaactcgtcg gcctcgggcg
     1201 gatgggtttt ggaggactcg tgcatggtgt tggagagcgg cagggagacg atgttgatga
     1261 cgaactctcc ggtctcctcg atgttgtcga gggtgtcctt agtgaattga tcgtcggagg
     1321 tgcgcggccc gatgcttatg cagagcatgg gcgggttacg ggatgcgacg gtgaagaagc
     1381 tgaagggcgc gaggttgcgg acgccgtccc ggctcacggt ggaggtccag gctatgggac
     1441 gcgggactac ggagcctatc aggagtttgt agacgctctg cgggtcgaga tcgcgggggt
     1501 ctatgatcac ggttggttac tttgccggga gacactcggg acagaggccg ccgagtagca
     1561 cgttcttcca gtcaaccatg aacttccggt cgccggagat cttcaacccc tcgacgccct
     1621 cgacgtgtac gtcgtagagc cggtcgcaac tccggcagcg gaagtgatgg tgggtggggt
     1681 ctggatccga atcgtagagc acggcgccgc ggctctcgat catccgcaag agtcccacat
     1741 ccacgagtac cgagagcgtg ttgtaaaccg tggcccgcga caactccggc aactcgtcgc
     1801 gggcacgccc gaacacctcg tcggctgtca ggtgctcgcc cgccccagcc tcaaaggcgg
     1861 accacacggc acgccgctgg ggcgtgatcc gcagccccgc atctctcaac ttctccaaat
     1921 acccttccct cgtcacgcaa ctctagagag atagtctact tggatttgat acgcagttca
     1981 ctccgaattg gatctcgccc cagagactcc gcatgccgta cgtaagaacc ggcgcgagac
     2041 gttccgctct cgcgccggtt ctttacttct cttacgatga gaaggctaac cgagcttcct
     2101 ggcgatctcg tctatctcct cggggctgac ggtcggggcg aacatctcgg ggaccggagt
     2161 aaggtccggg acctcgccgc cctcgggtat gccgtccacg acctcgcgct cctcgccggt
     2221 gtcggcctgc gggccgttcc agatctcccc catctgcctg taatcatcgg ggctgaagcg
     2281 gtacaggaaa cgatggtaac ccttgtccat gaacttcctg gcgtgcggat atttctcgct
     2341 ctgtatgtcc ggcaccggca gcaacttcgt cacgtccaca cccgagaggt cggagagcgc
     2401 cttggcgtag gcctcctggt ggacgccacc gcggactatg aggtacccga ccatctcgcg
     2461 ggccacgggg ttgtcggtgg tctcgtagac ccggatcttg cccatcctgg cgccggactc
     2521 caggaagaag ttgtggagca gatccagctt gaggttgccg gtggagaaga cgtagtcgcc
     2581 actccacttc gcgcccccgg cgccagcagc catggtgccg aggccggtgt tgaggaagtg
     2641 gtccgggtac ccggcgccca ggcccatccc caaaggcgca gttcccagag aaccgtccgc
     2701 gttctcgacg gtcttgtcga gcaggaggtt tatggtattc gccaccagct ctatgtgccc
     2761 catctcctcg gcggcgatgt tggcgaccag atcgtagtag ggccggatcc tgctcttacc
     2821 cctcatgttg aatgactgat aagtgtagtt catcaaagta gacatctcgc cgaacttgcc
     2881 gcccatgagc tcctgaaccg tcgccgccgc tgccgggtcg gcttccgagg gcctcggtaa
     2941 ctctacctgt aatttgtcta tgcgtagaaa catgctcctc ctgttcctga cccttcctcc
     3001 gtttgcgtag aaaattcgcc cgcaccactc gcgcggaacc tctcgtgaac ctcctctcgt
     3061 acgcctcgat atatttattc tctgtttaga tttcgtctaa acaaataaaa agtctaattt
     3121 aatgagcgcg ttaaggatcc gggctacgag gagtaaggct gaacatacgg aacatacaaa
     3181 gtgagacgcc gccctcccgg aaggaggacg gcgttccggt caggagggta cggatacctc
     3241 agaagtgagg tccggcctcc cgcacctcgt ctgagacctc ggcctcgaac ttcttgaagt
     3301 tctcccggaa gaggtctgag agcttcttgg cctgctcgtc gtaggcttcc ttgtcggacc
     3361 aggtctcgcg ggggtccagg atctccgtcg gcacgcccgg cacttctttg gggacgttga
     3421 ggccgaagaa ggggtccttc tccgttaccg cgtcgtcgag cttgcccccg atgacggcgc
     3481 ggaccatctc gcgggttgcg gcgatgtcca cgcggcttcc aaccccgtag ggaccgccac
     3541 tccatccggt gtttatgagg tagcagcggg tgccgtgctc gcgcagcctg tcggagagca
     3601 tcgtggagta ggtggtggcg ggcagcggca ggaacggcgc cccgaagcag gtggagaagg
     3661 tcgcctcaac gtccgtctcc atgtcggcct cggtgccggc gagcttggcg gtgtagccgg
     3721 agaggaagta gtaggcggcc tgctcgggcg tgagggcgct tatgggcggg agcaccccga
     3781 aggcatcggc ggtcaggaag aggacggcgg atgggtgtcc ggccatgccc tcggggacgg
     3841 cgccctggat gtgctccagg ggataggcgg cgcgggtatt ctcggtgagg gcgcggtccg
     3901 tgtagtcctc ttgccgggtc ttgcggtcca ttccgacgtt ttcgagcacc gctccgaaac
     3961 ggatggcgtc gtagatctgg ggttccttat cgggagagag gtctatggtc tttgcgtagc
     4021 agccgccctc gaagttgaag acgccttcat ccgaccagcc gtgctcgtcg tccccgatga
     4081 ggcgtctctc cgagtctgcg gagagggtgg tctttccggt gccggagagc ccgaagaaga
     4141 gtgccacgtc gtcattcggg ccgacgttgg ccgagcagtg catcgggaag acatcgtact
     4201 ccgtcgggag gacgaagttc agtacggaga agatcgactt cttgatctca ccggcatagc
     4261 gcgtgccgca gatgaggacg agcttgcgct cgaagtccag gccgacgaag gtctcggatt
     4321 cggtgccgtc ctcctccggg tcggtgaggt agccggggac cgagatgaca gtccactccg
     4381 gctcgaagga gcgaagctcc ccgcgatccg gacgccggaa gagctggcgg gcgaagagtg
     4441 cgtgccaggc gaactcggcg accacctgca cgttcagcct gtagcgcggg tcggccccgg
     4501 cgtaggcgtc cacgacgtag acttcgtcca ggttctccat gtaggcggag gctttttcga
     4561 gtatggcgtc gaaggcctcc ggcgagaagg gtttgttgac cgctccccag gcgacggtgt
     4621 ccctcgtcat ctcattctct acgatgaagc ggtctttcgg tgagcgtccg gtgcgtttac
     4681 cggtcttcgc taggagggcg ccggaagagg agatcatgcc ttccctgcgc cgcaccgccg
     4741 cctcgacgag cctggcggga ggcaggttct catgcaccgc tcccaggtgc gtcagcccga
     4801 agcgttcgag cgtcgtcttg cgttcagcta catccatgca tttccctttc accctttaca
     4861 acccgaccat gttgggccgc aaggcgcaca ccggttcatg ccgacgcagc ggcgccatcc
     4921 gccgcccgaa ttctaacaaa cgaccgccag ggtcgccgaa gggcggggcc gaatcggctc
     4981 ccacgaagca cgaacctgcc gcatcgagtc cgacgctccg agcgcgcgaa gtggccgggt
     5041 tactggcctt ccgccgcaaa cgcacagcag gcatacgcct tcccggctag atgacggcga
     5101 gcgccgctac cacaaggcta actatcatat ccgcccacga aacgcgtaga gagagccttt
     5161 gaagatcacg gcgccgcatc gccagagcgc gagcctcttc cccactcccg gcctgacgtt
     5221 cggcctcggc gagaccggcc agcttgcggc cgaagtaggc tccgtgggcg gcgaccaggc
     5281 cgagcagcac cgcgagcagc aggtactcca cgtagcgcac gaccccgaag cccgaggaga
     5341 gtccgtcgag caaggcaaat attacgagca ggatcaggtt gacgctcccc accctggcgt
     5401 actgcttccc gatcctggaa cgcaccgaca cgcgctcgtg ctcctcccac ttcatagcct
     5461 tgagcgccgg tgagacgacc gccgtcgtga agaccacgcc acccagccat acccccgcga
     5521 gtattgcgtg cgcggcgtga aaggtcacgc tcaacatctc cacctccttc cgtttcggga
     5581 actccgtcgg gaactccggg cccgctttgc caggaagcaa atccttgatc gcttgccatt
     5641 gagcttccgt gagttcgcac cttttgggca tatccacctc actcgttggc ctctctcttg
     5701 ctgagagaaa gagatgttac cctatatgtc gatttgccct agaacatata gctccttacc
     5761 gactgctagg cctcgaaagc tatgccggag gggttctggg tgcggaagtc ttcgagggtc
     5821 tgggcatcga ggtagtcgag gatggcttgc tggccttctt ccagggcctc atgcagggcg
     5881 catggagcgc cctcgccgca gttgcggttt tgcatgatac agcgcgtaac ggcgaaaggt
     5941 ccttcgaccg cttccacggc ttcgcggagg gatatgtcgc gcgccggccg cgtcaggcgg
     6001 gagcctccgc cgcgaccctg ctcgctctcg accaacccgg cgcgggagag gttcgcaagt
     6061 attctggcga gtagacgccg cgggatgtgg gcgtcggccg agatcctgtc cgccgtcacc
     6121 ctctcccccg tccacgcgag atagagcaaa gccctcaaag cgtatcgccc ctcgctactc
     6181 aactccaacc gcaagacatc cacctctcca cagaaacccg caaccatccg gcagatccat
     6241 gtacccaccc gagtcgccat aagatacacg ccaaccagag gcgcaatacc cgaacgttcc
     6301 agacgctttg attactgtat ttgtaataat atatcgacgg cgggcactcc atacacgacg
     6361 aaggaggcgg tggagatgac gaccagacct catccggaat ccgcgcgtcc catgcacggt
     6421 gcgcttcaac gctttgacct ggagggtgaa gtctctcgtc tgcgcggcga gaaggagtgg
     6481 cgtgagggcg ggcgcaacgc catcacgctg cgcaagggag gcggtttgag cgtggtgttg
     6541 ctggtgatga aggccggtga ccggctggag gagcacgtcg cgcccggtcc gatctcactc
     6601 agcgtccacg agggccgcat caggttctcg gccgctggcg agacggtgga ggccggaacc
     6661 gggacggtgc tcacgtgcga cgccggggtg cgtcattccg tggaggccct cggcgacgcg
     6721 gtgtgcctgc taaacgtgac ggccggaggc gaggtgtggg atcccgaatg agcgggatgg
     6781 ctccgtggat ctgaccaaga cggctgcgcg agccgaagac cggaatggcg tcgtatgggc
     6841 actggaagag agcggcgacc tgaacgcgaa cctggtgcgg ttcggcaccg gtcgcggcgt
     6901 cggagaacac gtcaacgacg aggtcgacgt actgttcctg ggcgtgtcgg gagcaggatt
     6961 cgtcatcgta gataacgaga agcacgccct ctcggcgggg aagctcgtct tcgcgccaag
     7021 ggggacccgg cgctccacca tcggagcctc ggacgaattc tcctatctca ccgtacatcg
     7081 ccgccgggga ccgctccgga tctccgaacg gatctccgaa aagtagcccg tcaagctccg
     7141 cgcccggcag agtacctcgc ctgacttgcc gctggtattc gccgcctgta gatttccggt
     7201 gcggttgcaa agttcgggct ttgtaggagt tggtcttggg tgtcgggcgc gaaagagatt
     7261 ctcggcggct cgccggcgag ggaccgggga ggaagaaaat ggtgtagacg cgggacccag
     7321 ggatctacgg ctatcctctc taggtgattt acgtgaaagg agtcgacaga tggacgtaac
     7381 tgcggcggtg gttcgcgaga aagagcagcc tttcgtcatc gaggagcttg ctctggagga
     7441 acctcgggca gacgaggtac tggtgcggat cgtaggggcg ggggtgtgcc acacggacct
     7501 gatcgtgcgc gaccagtggt atccggtgcc gctgcccgcc gtgctcggcc atgagggcgc
     7561 gggcgtggtc gagaaggtcg gcgacggcgt gacgaaggtt cagcctggtg accacgtcgt
     7621 attgagctac cgcgcgtgcg gcaagtgcat caactgcctg cgcggaaagc cgccctactg
     7681 cctcaagctc tacgaagaga acttcggcgg cgcgcgcacc gacggctcta acgccctgag
     7741 caaaggcagc gagaccgtcc acggtcactt cttcggccag tcctcgttcg ccacctactc
     7801 attggcggcg gagcgcaacg tggtgaaggt acgcgatgac gtaccgctgg agttgctcgg
     7861 tcccctcgga tgcggcatcc agacgggtgc gggcggcgtg ctcaactccc tgcacccgga
     7921 ggccggcagc agcatagccg tgttcgggac ggggtccgtg gggatgagcg cgatcatggc
     7981 cgccagggtc gccgggtgcg ccacgatcat cggcgtggac gtgaaaccaa accggctgga
     8041 gcttgcgaag gagctcggcg cgacccacac cataaacgcg gcggagtccg ggaacgtggt
     8101 cgaggagatc cagggtatca ccggcggcgg cgcggactac acggtcgaga cgacggcctc
     8161 cccgcaggtc ttccgccagg cggtagactc gctacagctc ctcggggtgt gcgggctcat
     8221 cggggcggcg gcgctgggga cggaggcgac gctcgacatg aacaacatcc tcttcggcag
     8281 gacggttcgc gggatcatcg agggagactc cataccggac atcttcatcc cgcgcctcat
     8341 agagctatat gcccagggac gcttcccgtt cgaccgcctg atccggttct acgagctgga
     8401 cgagatcaac caggccgccg aagactccga gagcggcgag gtctttaaac ccgtcctgag
     8461 gatgccacag ggcgcgtgac ggaccccgag gaacgccgga cctatctccg ggcgcgcggc
     8521 taggttccgg actatcacaa ggtgctggcc gagcaggact tcgaggtcct gcgttccttc
     8581 aacgccattc tggaataggt ctacctgaac gagcggcgcc tcgaccgcaa gacgaagaga
     8641 agctccttcg tcctcagtct gacggcggcg cgctcgaaga aggcccacat aaagagtccc
     8701 gtcagggcgg ccctcgacgc gggcgggacg aagagcgcgg tactggaggc gggcgttgtc
     8761 gcatttcaga acagcttcga cgcctggtgc gaagtggctg acgctaccgg catagggcca
     8821 accgtggagg cttacgaagg aggacaaggt cttcgcgtag ctatccaggc tgcgcgacgc
     8881 ggggttcggc ggcatccgcc cgccgggcct cgcggacccg ccggatcagg agcgtaaggc
     8941 tcacgagagc taccagaagg ctcaggaact ccacgccgag atatccggcg agggtcgcgc
     9001 tcctgacgcc cgcgaaggtg tcccagagtg cgtgcagtat cacggccgtc aggaaggcgc
     9061 cgacgacccg ccgatccagc ctcgcgtgtc cggccttcac ccgttcgcgc cacaggacgg
     9121 cgcagactat gccggtccag gcggcgtgtc cggccggaga ggtcaggccg cgcaggagga
     9181 gaacctcgtc cagcgcaccc aggttgccct tcgactggag gagcgtgacg aagccgtaac
     9241 ccatcgtctc cagcgccgcg aagcccatcg cggtcgcgac gccgagcact atcccggcgg
     9301 cctccgaacg gtagcgtccc ataaagtaga agacgagcgg gacgatcagc ttcgctccct
     9361 cctcgatgaa ccctataaac agctttggca cgaagccgag cgttctcgcc acgtcgtact
     9421 ccagcgtccc ggcgaccacc gtgccgagca caccgcccca caggaagcag acggccagcg
     9481 agggtaagga tatctcccac ttcggcagcc gctcgtagag gtaggtgacg aaggtaacgg
     9541 gcacgaggaa cgcgccgagc aggatgacgg aagggatata gttcgggttc cccgtgccga
     9601 cgagcactcg ctccaccagg tagagcaaga cgaagccgct gaagaagatc ctgacccacc
     9661 cgcgcttcag aaaacccatc tcaccgatcc cggcatcgcc gacttctccg ccgacacttt
     9721 atcgcgtccc ggttcggccg gtcgtgcagt gactcatata cggagcgtag ggtagaagac
     9781 ggcggccagc ggtatcggga gccagaagtt gaagagccgg tacccgagca cgggtatggc
     9841 ggcttcggag cccacgccga gcagggcgag ggtggcgatc atggtggcct cgaagacgcc
     9901 gatgccgccg ggggtgaggg gcagcgtccc ggcggcggtg gctataccgt atgccaccaa
     9961 aagctccacg gtccccgccg gtacgccgag ggcgtggaac atgacgacca ggcagagcgc
    10021 gtcgaagacc cagtagccga gcgccagtcc cgcgagcccg aacgtcgaac gagcccggct
    10081 aatgtactgt ctcctggcgg ccttgatctc gtccctgacc gtagacacca cagtggcggc
    10141 ccagctttcc accgtccgcc gggaccactc gcgccggaac agcctgccgc cgaggagcag
    10201 aatacgagcg agaacgcgcc gggtcagcgc ggggttgcgg tacgcctcgt agctgccggc
    10261 gcctgctacc acgacgaaga tgatcccgac aagggcgacg ctcgtctcgg ccacgcccag
    10321 gctctcgttc aggaccatat agagcaggct cccggcgaag atcgcaccca gagccccgta
    10381 aaccagggcg gagacggccg ccaccgcgag ggctatcttc ttgcgctcga agccccgcga
    10441 cctcagcgcc gcgtaggtaa ccgtcgccgc cgtcgctccc ccgcccggca ggacgtgtgc
    10501 ggcgccataa ccggtcacgg cgaggcgcag cgtgaaccac aacccgatgc cccggtgccg
    10561 ccggtagcgc agataaggcc cgagtccgga cgacatcatc accgagcgcc ccagcagatc
    10621 cgcgtagcac aattcactca gaagctccgc taggaaggcc agcaggatta gcgccggcgc
    10681 cgcttccatc atcagatcca cggacctcct caagcccggt atctgcggca gcatcagatg
    10741 cagcgcaagg ccgatggacg cgacgtagag cacgccgcgc gcgaacctcc gcctcatctc
    10801 gccgcttaac cgcatccaga gccctcccag aaggaatacc gcaccgcatt atggcagagc
    10861 ggccggcgag acctcctgga atgtccttca ggatgacctg acagagcgct tcagcatttc
    10921 agcttcgcgg tggtttgctt aagagacctt tgcgagacgg cgtgctgtct aactaactgc
    10981 ggtctccgcc tgcgccgtcc ttcgcccggc cagcctctcg gccgacgctg ggtcgtggcc
    11041 cggcaggtac acggtctggt tctcctctgc gtagcgcaga atgcgtccca gggtccgccg
    11101 ggccgcattg ccgcttggcg aaaccccgtc tatggcctgc tcgagcatca gttcctccgt
    11161 gtaggaggca tcgcctgcga ggaatagcga gcgtcccttc tccaggacca ccaccgatac
    11221 gtgtcccgcg ctatgacctt cggtcccgac cagccacacg tcgcccgctt gggtaagggc
    11281 caggctacga gggaaggggc cgaacggttc cagttcgagg tccaccaagc ggggggcgaa
    11341 ccaggaaggc caacgatgcg gcaggtagcc cgccgccttc cccaaaggtc ccgtcgcaat
    11401 gcgatgctcc tcacgggaga cgaggatttc ggcgttcgga aaatggtgga gtcccccggc
    11461 atgatcgaga tgcaggtggg tcaggaccac ccggcgcacc tccccgggct ctatccccag
    11521 gctgtgcagc cggggtccaa tctcctcctc cgggcgcacc gagacccgca cgttgcgccg
    11581 gaagtacgga ttccaccggg taaaataacc cggctccagc atccgcgtgt ttgcaccggt
    11641 gtcgaccagg atcacaccct ccggatgctc aatgacccag caccagatgg gcaactgctc
    11701 cgtccacggc ccacccaacg cggtcctcag caagcgcaat gctccacggc cttcacccct
    11761 gcgctgtctt tccctgatcg ccacagtccc tgtctgtgcc gcgcgtaccc gcatcgtccg
    11821 ctccttcctg cctacgcggc catcagccac gcagccatca tctacatgac acatcatcta
    11881 cattaccatt tacgtgacgt gtcatcaaca tattcgacca gcttttgcta aaattggtga
    11941 catgtcacgt tgccacacga tcgcgcacgc caggtaagag gttcctcacg acatgaatga
    12001 gatcgaggag ttgcgctacc tggttttggc ggcccagcgt gaggggaacc ggatgctaac
    12061 tcagaggcta cgccctctcg gactgactgc ttcgcaggcg gaggtcctgc gcgtgctgca
    12121 agatcaccaa ccgctatccc ttatagagct cggcgggcta ctggtgtgcg agacgggcag
    12181 ccccagtcga ctggtcggcg ggctagtcga ggccgggcta gtcgagcgga cgctgtcgga
    12241 ggccgacaag cgaatgatca ccctttcgct cactcgcgag ggcgagcaga ccgcctcaga
    12301 ggtcgctgag gtcgaggcgg cactttaccg gacgatcacc gaagctctga acagcgaaga
    12361 aatctcagga gtcttagctg ccctgcggcg gctggtagcg ggccgcccag ctggcgaagc
    12421 gctgtcccgg cgttcgcgac tttcccgcct gccttgaggc cccgtactcc aatgtgaata
    12481 gagcactcca gaaagtttga ccctacttgc atgttcgcag agccgtcgca tctgcattac
    12541 gtcttgaaac gcgctcctag ccgcgcaccc gctcctccac cttctctacg tactggtccg
    12601 gcttcgactt acgctccagg ccaccggcgg acatgtagcg aaccttgccg tagacggcgc
    12661 gctcggtcca gccccggtcg tgctggccga agacccaggc gacgttggcg aaggagttgg
    12721 ggtcgcggcc gtcgaggaag tacttgttgt tgaggtagag cgttgtccgg taagcgtgct
    12781 cgggggtgtt ggaccactcc aggatcttct tcccccagta catccgcatg tagttatgca
    12841 tgtagccggt gtggatcatc tcccgcatcg ccgcgttcca gtactcgtcg tgcgtcccgg
    12901 cgctctctag ctgcttcgcg gtgtaggtgt actctcggtc gtctttcttg tgctcctcca
    12961 gcgtcttctt cgcccagccg gggaggcagg agtaggagtc gtagtcgggg gtgtagtgga
    13021 cgaagttcat gcttagctcg cggcgcacga tcacctcctc gacgaacgag tctatgttct
    13081 tcttgtccgc gttagactcc tgagcttcga gggcgagcca gaccggggag acgtgcccga
    13141 agtggaggta cttgctcatg tgcgagacga aatccgtctg cggctggttg cggttggcct
    13201 cgtagtcctg aaaactcttc ttcaggaagc ggcggaagac cttcttcgcc tccgaagtcc
    13261 cgccccggta caggtggctc aacacgccca cgctccggtc gaggtccatg ccgtccagca
    13321 ccttctcgat gtccgagaga tccaggccag cggcgtccat gtttagcgac tgcttctcgg
    13381 gcttcgtggg cttcagttcg accaggaagt cttcgaggta ctcgtggatc ctgggccgca
    13441 gcgtcctcgc ggcgtgctcc tgcttgccgg aggcgagctc gacgggcacg accacgtcgg
    13501 tctcgacctg caccacccgg caacccgcct cgttagccac cttcttccgc cactccttct
    13561 gcgggcgcag gtagccgcgg tcggtgacga tgagagaggc gtctttcccc gcttcgagcg
    13621 cgacctcgtc cggcgatccc tttcggacgg cgagctttat gccgcgtttg ttgagcgcgt
    13681 cctccacgtc ttgcagacct tcaagcatga aggcgtagtg gcggaggttg gcttccgggt
    13741 agtcgtccgt gaggccgaag acgacgagca ggctttggcc gaagtcgttc gcctgccgga
    13801 cggcgtattc gagggcgtgg ttgtactcgg cccgctgtga ggactgcatc cagtagagga
    13861 cgtaatcacc gtcccgcgcg tcctcttcgt tgaggtcttg gatgcgttct tcctggatct
    13921 tcgtggtagc catgtgcgcc gtcctccggg gtgttggtct attcgcaaag cataggagga
    13981 ctgctccgcc gcggatgggt taggaaacac agtccgccga agcgggctat ttagatttcg
    14041 agggggcttt ctcgaactcg gcggcggcgt gggagcgcca ctcgaggagg tcgttgcggc
    14101 gctggtccag ttcggcgggc ggtatctggc cgatgatatt gtgcttctcc agcgggtcgt
    14161 tcttcaggtc gtagaactcg tcgggctgct tgccgtagta gtagatgtac ttctggtatc
    14221 ccttgatgct cgccatgcac tggaggtccg ggcggcagtt gaagtacagg gtgcggccct
    14281 cgggagattt cagcgcggag gcgccggggt aatcgccgtt tacgaccctg tagtgaagca
    14341 tgtccaggat ggtcggcggg aggtctatgt ggtgtatggg agcgtcggcg ctgatcttcg
    14401 cgccgttctg ccagcgctgg gggtcgtgga tgatgagcgg cacccgcagg ccctcctcgt
    14461 agacgacgcc gtcgtgcccc ttcacgccgt gctccccgaa tgcctcgccg tggtcgccgt
    14521 agatgatgaa gatagtgttc ttgtacagac cgagcttctt gtactgttcg atcaggttct
    14581 ttatccagaa gtcgtcgtaa cggacggcgt tcaggtagcg gttgagcacg tcgtctttgg
    14641 cgagcggcac gcgcccgtag cgggtgggcg cgaggtactc gtggtgaggc gtgatcccga
    14701 tgtaactcgc cataaaaggc ctgtccttgt ggctcgtgag ccactcttcg ctgggcttga
    14761 gcagaatgtc gtcctcgtag cccaggtagc cggagcgctg gaagcccttc ttgctcattt
    14821 ccttcaggcc gacgaagtcc tcgtagccga agttcttggc gagctgcggg cggttctcga
    14881 acgtcgcgat ggccgactgg aacatgacgg tgttgtagcc ctgctccttc agcagctccg
    14941 gcaggcactt gaccggcaca ccgcccggct cggcctcgtg tatgtccgtg gacgggtccg
    15001 ggtagatgcc gcaatttatc gaggtcaggg cctttgaggt gtgcggggtg gtcgtgtagg
    15061 cccgttcgac cagcaggctc ttcttcgaga gctcgtccag gaacggcgtg gtctgaaggt
    15121 ccgggttgta gggcgtgacg gagcgctcgc gggtggattc caggtggatc aggacgacgt
    15181 tccgcttctc ggtccggggg gtctccttga gcttcacgtc cgtgagaggg acggagacgg
    15241 aggctcccgc cttctcgacc tcgagttcgt cggcgccagg agcggcgatg cccgtgatga
    15301 ggacgttggt cagggggctg agggagaaag agcggtcgga gttttcggcg ccggggacca
    15361 tcgagagcga ggcgatgccg acggcgatca ggcacagccc cgccgcctca agccgggagg
    15421 tccgcgcagt cccttcatga ctccggcgcg cggcgaggcg cgtgatggcc cagggaccga
    15481 gagcggcgta gagcagggcc aggatcaggc cgagccaaac gtaccaggcg gcctcgctgg
    15541 cgatgatctc ctttacctct ccgagggtgc tcaggtagaa ggtgatgacg ctgtagtcga
    15601 gggtggagcc cgtaccctcg aagtactggt aagcgaccgt cgagacggtg acgatgagca
    15661 gcgagatcac gtgaaagaag actacagcga ccttgcgtag cgctccccgt cgggcgacgg
    15721 cgaagagtcc gacccagagt atcccgtatc ccacgttgaa gaggatgtcg gagcgcatga
    15781 ggtcgaggct tccccacaag cccggcgcat cgtccttgat ggcgatgctc atggccttta
    15841 gggtcaggtt gtagacggtg agcggaacga ggaggctgag gaggtagatc caatccctcc
    15901 cgctcaacaa ctcccggata gtcttctgaa acgcgccgac catgaagacg gataatatac
    15961 cgcatacctc cagatcgccc atccgggcgg ctgggagatt aagttctggc cgactcctag
    16021 tccggcttcg actcgggctc gaaccggagc gcggcggagt ttatgcagta acgcaggccc
    16081 gtgggggcgg ggccgtcatc gaagacgtgg ccctggtgcg cgccgcaggt cgcgcagtgg
    16141 acctccgtgc ggcgcatgaa gaaggaccgg tcgctctccg tctccacgtt accctcgtca
    16201 accggcgccc agaagctcgg ccacccggtc ccggactcga acttggtatc tgatgagaag
    16261 agcggcgtcc cgcaagctac gcacaggtag gtgccgtccg ccttgttgtc gttgtactca
    16321 ccggtaaagg gccgctcggt gcctttcatc cgcgtcacac ggtactgctc ggaggtgagc
    16381 tggtcctgcc actcctcttc ggtcttctgg atcttcctcg gcgtatcagt catgccgaaa
    16441 ttttagcacg cggatagtca cctaaagtgc gcgctccgtg cccagtaccg ccgttacctg
    16501 gctggagagc acaccgccgt tgccgtgaac gagggcgacg ttcgcgtcct ctacctgccg
    16561 catgccgcac tctccccgga gctggcgcac ggcctcgacg atgaggagca gaccgtacat
    16621 ccccggatgg ttgtaggaga gcccgccgcc gctggtgttt accgcgagct caccacccgg
    16681 cgctatgcgt ccgccggaga cgaacgctcc cccctctccc ttccggcaga acccgaggtc
    16741 ttcgaggaac atcacggtgt ttatggtgaa ggcgtcgtag agcatcgcca cgtccacgtc
    16801 ggcgggaccg agccccgcca tcccgaacgc ccgcggcccg gactcggcgg cggcgctcgt
    16861 ggtcaggtcc ggcatctggc ttatgtggcg gtgccagtgc gcctcccccg ctccgagcag
    16921 gtatatcggt gtcttcttga ggtctcgcgc ccgctccgca ctcgtgacta tcgcggcgca
    16981 gccgccgtcg gtgacgaggc agcagtcccg gacggtgaga ggcgctgaga ccatgcggct
    17041 attcaggaca tcttcgacgg tcaggtcgtc gcgcatgaat gccttggggt tgagcttcgc
    17101 ccattctctc gcggcgacgg ctacctcggc gagttgctcg cgggtcgtgc cgtactcgtg
    17161 catatggcgg gaggcggcga gggcgtacat gctgacggga tagcgggggt tgtagggcgc
    17221 ctcgtatggg agagtctcgg agccggagac gaggcgaccg ccgccggagc gctgggtgga
    17281 gccgtaggtg atcagggcga cctcgcagag cccggcgtct atggcggccg cggcgtggaa
    17341 gaggtgggag acgaaggatg agccgccgat gctggtggcg tccgtgtagc ggggccggat
    17401 gccgaggtac tcgcccgcgg agagggtcgg catgtggtag taggcggagg cggagaagag
    17461 cccgtccacg tcggactttt cgaggccggc gtcgtcgaga gcccggaggg ttgcctgccc
    17521 gatcaggtcg agcggcgtaa gccccggccc gacctcgccg agatcgctct ccgccacacc
    17581 aactatggcc gctctcccgc gcaggctcat ccggctccat cctccagctc ggcatccggc
    17641 acgaagacgg cgaccggctc gtcgccctcc cggcgcaccc ggagggccac cctcgccccg
    17701 atcccgactt ccttcgccga cactccctcg acccggctca tcatcctgaa gccctcgtcc
    17761 aggtcaacga gcgctacgtt gtacggttcg gcgtcgcggc ggtgtacggc ggtcgtcgcg
    17821 tagaccgtcc ctcgtccgct gctcgaccgc cattcgaggg ttccgctccc gcaggccggg
    17881 cagaggactc tggggtagaa gacggcagcg ccgcaacccc ggcagagctg gaagccgagc
    17941 ttcccttcct ctagatagcg gtggtaggtc tctgcgggcg ctggctcgtt cttccggttc
    18001 acgggttacg gtctcctggg gctgccggac acgcggacag gcatcttcta tgagcccgga
    18061 gtctggcgca agttccggcg cggttgcgcg tggtgcatag ggcgtgcgag tatgatgcct
    18121 tattcgggca gggctatcga gtcgagtaga gaggagcacc gatggctacg ggaaccacgg
    18181 aagcgcgtag gggaccagag gtccatcatc tcaagaggga tgtaagcctt atcgggttgc
    18241 tcttcgtatc gctggggtcg atcatcgggt ccgggtggtt gttcggcgcg ttgttcgcgg
    18301 cccagatcgc cggtccggcg gcgctgctgg cctggatcgg cggcggaata gggatgctgc
    18361 tacttgccct gatccacgcc gagctgggag gcatgtatcc ggttgcgggc ggctcggccc
    18421 ggtaccctca cttctcctac gggagcctgg tggggttcgc catcgggtgg ataacctggg
    18481 tcggggcggt aacggtcgct cccatcgagg tcgaggccgc gctgcaatac gcggccaact
    18541 acttcccctg gctcaccagc acggcgggcg gcgtgaccgt actcaccgga ccgggcatcg
    18601 ccgtggcagc ggtgctgatg ctcctgttca ccatagtcaa cctcttcggc gtcaagctgc
    18661 tggcggagtc gaataacgtg atcatgatct ggaagatcgc gatcccgttc ctggcgatca
    18721 tcgtgatcat gctcctggca ttccacacct cgaacttcac cgcgaagggc ggctttatgc
    18781 cgttcggcgt ccagagcata ctgagcgcca tctcgaccgg gggtattatc ttcgcctacc
    18841 agggcttcga gcaggccatc cagctcgggg gcgagacgaa caacccgcag cgaaacatcc
    18901 cgatcgcggt catcggggcg atgctggtcg gcgtcgtgat gtacatcctc ttgcaggtcg
    18961 ccttcctggg cgccctggac cccaagaacc tctccgaagg ctggacgaag ctctcgttcg
    19021 aggggctggc cggcccgttc gcgggcctgg cgacggcgat cggggccggg tggctggcga
    19081 cgctgctata tatagacgcg gcggtctcgc cgggcgggac cggcctgatc tacacgggtt
    19141 ccagctcgcg cctctctttc gcgatggccc gcaaccacta cataccggcg ttcttcggac
    19201 ggctcagcga gaggggcgtc ccgacgatca gcgtcatatt cagctttatc gtcggcatga
    19261 tcatgttcct cccgttcccc ggatggcagg agctggtcgg ctttatcacc tcggccaccg
    19321 tgatgggcta cgccacggcg ccgctggcgt tcggcgccct ccgccgccag gagccggacc
    19381 acccgcggcc cttcaacttg cccggcggcg cggtcctggc tcctcttgcg ttcgttatcg
    19441 cgaacctgat catctactgg accggctggc cagtgctctg gaagctgttc gtcgccatcg
    19501 ccctcggctt cgtcctcctc gccatagccc aggcgacgag ctcctcggac aggaccccga
    19561 ccctcgactg gaggtccgcc tcctggttgt ggccgtacct tgccgggatg gcgatcatct
    19621 cctacctcgg cgcgttcgac gggataaagg tcatcccgtt cggatgggac atcgtggtga
    19681 tgacggtctt tagcctcgct atctacttct tcgccatgtc cgtccgcctc caccccgacg
    19741 aggtgcgccg ccacgtcgcc gacgcgcgcg aagaggccgc tgtggaagag gaactcgcgg
    19801 tatagacgcg acagcctgac aggccgccca cttgaggccg ggactcgtac ccggcctcct
    19861 tttctcgaac tattcgaggt cggcgagagc tttttcgtgg acttcgaggt cccgctcgcc
    19921 gtgcaggacg aatcggacga ggcgcacgtt ttcgagcgct cccgacgtct cggctaccgt
    19981 cttcagggcg actgccgcgg cctcctcgac cgggtagccg aagatgccgg tcgagatggc
    20041 cgggaacgcc acggactcta tgccccgctc atccgcgagg gtgagcgagt tgcgatagca
    20101 gtccgcgagc agctccgcct ccgggcggtc ctggccgtag acgggaccga gggtgtggat
    20161 gacgtgacgg ttcggcaggt cgtggccgcc ggttatgacg gcctcgccgg gccttatcgg
    20221 ggcgagcggc cggcactcct cggccagacc cggtccggct gcgcggtgga tggccccggc
    20281 gacacccccg ccaggcatca gccccgcgtt cgccgcgttg acgacggcgg ttacgtcgcg
    20341 ctgggcggct atgtctcccc ggacgcactc gatggtgacg ccatttatct cccgcttcat
    20401 cctgtcgcct ccacggagaa cacggaccgg ccgcagcgcc acacggcctc cacggagagg
    20461 tcgggggcga gcaccgtcac gtcggcgtcg tagccggggg tgaggcggcc cttgcgcttc
    20521 ccctcgccga tgagccgggc gggcgtggtg gaggccatgc ggacggcctc ggggatggtg
    20581 cagccggtga aggccaggat gttctggaag gcccggtcca tcgtgaggat gctgccggct
    20641 atggcgccgc tgccgagcct ggggacgccg ctctccaggt agaccgtgcg ggtcgcgagc
    20701 cggtactctc cggcccccat cccggcggcg gagatggcgt cggtagtaag gtagatgcgg
    20761 ttcggtccga gcattctgaa ggccaggccc acgacctccg ggtggacgtg caggccgtcg
    20821 gggatgagcc cgcagacgac gagcgggtgg gcgaaggcgg cgccggggat gccgggctcg
    20881 cggtggtgca gcgggctcat ggcgttgaac aggtgcgtca cgccggagac ctgatggtcg
    20941 aaagcttcgt aggcgagtac gaaagaggcg tcggagtgtc cggcggagac ggcggtcccg
    21001 cggtcgcgcg cccgctccat caactccccg gcgcccggta gctcgggggc gagcgtgagc
    21061 atccgcacgg gaccgaggtc gaggaggcgt tccagcagcg cgacgttcgg cgggttcagg
    21121 tgggcggcgg cgtgggcgcc gcgcttctcc aggttcagga acgggccttc caggtggatg
    21181 ccgaggactt cggcgccgga tgcaggatcg ccggtcctcg ccgccagctc cggcagcgcc
    21241 cgctcgtaga gggactcggg agaggagatc acagtcggaa gataagacgt tgtgccggtc
    21301 tggagcagcg cctccgagag ctcatccagg cgccctggct cggtcgccac gtccaccccg
    21361 aaggagccgt tcacctgaag gtccacgaac ccggggacga tgagcgagtc cgggtagtcg
    21421 tggacctcgt cggcctcctc ggcgagcgac ccgtcgcggc cgatgtcccg gatggatccg
    21481 ccctcgatca ggacggtcgc ctccggccag acctcgtagt ccgttaccac ccttccccgc
    21541 aacgcgacga gtttcacgat accctcctcc cttcctctct gttatgccga cgtatcaacg
    21601 gtgcccgcct gttctcgtac tgcgtcttcc tcatacccgg cgccgctctc tccgggacgg
    21661 acgtcgggga cgcggaggag gaagtagacg ccaagggcca tgatcaggga gagcgacgct
    21721 atggagatgc ggtatgccag ccctccaagc ccgtcgagaa cgaatagcag gatggccgtg
    21781 agcgccggtc ccgtgacggc tgagactttt ccggcgagcg aaaagaggcc gaagaactcg
    21841 cccagcttct ccggcggcga gagtgcgatg agcatgaccc ggctgacggt ccacgtcccg
    21901 ccgagagcta tcccgacgag aggccccgcc aggtagagca tccagggccc cagagcggcg
    21961 gcggcgagcg cgatggagac gagccacagc ccgaggaccg cgagcagcgt cctcttcggg
    22021 ccgtaccggt ccgtggcgaa accgcagatc gtcgagccca cgaccgcaaa gaccgtcgag
    22081 aagaggagaa ggttgcgtat ctccccgctg tccatgtcga agaccttgcg gccgtagagc
    22141 gccatgttgg agatggcggt gttggccgcg tcggtgtaga ggatggtcgc gatgatgaac
    22201 gtgccgacgc ccgcgtagcg gcgcaggttc ttcagggtcc gcaccacgtc ccggtacgcg
    22261 atcccgagcg agacgggccg cggcgcgcgc acggccgggt cggggacgaa gaagaagacc
    22321 ggcaggctaa agaggaggta gaggacggcc gtcgggagga aggcgttcga gctgttctcc
    22381 ccgctgatct cgatccaacc gcccagcgga cccatcaaag agtggacggc cccggcctgg
    22441 tccacgaaca ccgtcaggac cagcagcgcg aagatagagc cgacgtaccc ggcggcggtg
    22501 ccgtagccgc tgatcctgcc gtccccacgc ccagcagcga ctaccggcaa aagcgcgttg
    22561 tagaagacga gcgccgactg gtacgtcagg tccgccgcca cgaagagcgc cacccccacg
    22621 acgacgcccc cggagacgcc cagtcccgcc gtcatgaaaa ccgagatcac ggtgagcacc
    22681 acgaggtacg ggaccctccg ctgccggaag tcggccatcg cgccgagcac gggagcggta
    22741 cagagcacca gcagcgccga gacggcgacg gcgctgttga ccagccagcc gccgttcccg
    22801 atctcctcgt ccacccacaa tgggaagaag aacgagagga tgcttataga gaagatggta
    22861 ttggcgaagt cgtagaggac ccaggaccag accgcgagcc tggaagccct gaagccggtg
    22921 gtcatcagcg gctagttatc ggatgtcgtc tgcgccctct ctccacaccg gcagtataag
    22981 ccagccggcg ccgcggctca tcaacgccgg tacgggagcc tagcccagcg ccacgtcgag
    23041 ggtcatcatg acggcgaaac cgaccattac ggacatggcg gcgatatccg ggctgccgcg
    23101 tttggcctcc ggcaccagtt cctcggcgac cacgaagatc atggctccag cggcgaacgc
    23161 cagggcgtag ggcaacatcg gcttcgccag cacgacggcc gccgcgccca ggaccccggc
    23221 gaccggctcg accaccgcgg agagctggcc gtagaagaag ctctttccgc gggacatccc
    23281 ctctcgcctg agcggcatgg cgaccgccgt cccctccggg aagttctgca gcccgatgcc
    23341 gacggcgagc gccacggccg cgcccagcga agcgtcctgg atgccggatg ccgccgcgcc
    23401 gaacgccacg cccacggcga ggccctccgg gatgttgtgg agcgtgatag ccgagacgag
    23461 caacacgctc ctctgccagc ccgtcttgat gccctcgcta tcggacatct cgtgccccgg
    23521 atgtaggtgc ggcagcacga ggtccaggcc ccgcaggaaa gtccctccgg cgagaaaccc
    23581 ggcgagcgga ggtatccaca tcggcaaagg tccgttcttc gagagcgaga tcgcgggcgc
    23641 cagcagcgac cagaagcttg ccgcgatcat gacccccgcc gcgaacccca gcatcgagtc
    23701 gagcagcttg cggttgatgt ccttcgtccc gaagaccagc gccgccccaa gcgccgtaag
    23761 cccccaggta aacgtcgtcg ctaccagtgc ctgcaacacc ggcggcagcc cggacaagaa
    23821 ctctatcatc gccggttacc cggctcgttg aatgaccggc cttcgaagct cacggctgaa
    23881 ttcatggctg aattaatagc acacccggct ccagagccgg agaacaggtg tcgtgaaaca
    23941 acgcggccac cgaacgcggc caccgaacgc ggctaccgtg aagctgcggg aggccgaaga
    24001 cgtccgatcc ccggcacgaa acggccgacc cgggaggcgt accgggcgta ctcgtcgccg
    24061 tgagtcctga gcagatacgg ttcttcgacg agccggacct gcatctgaag agcgacgaac
    24121 agcgccgcga aaccggcgag agcagcgagg ttgggcacga ggagagcgag ccccagcgat
    24181 gtcgggatca ccgccgcgaa gatcgggttg cgtacgaccg cgaacggtcc tccggtcacg
    24241 agccgcgtct tttcccgctc atcgacccct atgcgccagg agtcgcccat catcagttga
    24301 gcggtcaggg tgccggacag cccaacgagg tagagcgcga gaccggagaa aagcgcgggc
    24361 ggaaagagcg ggaccagcgc gccggcgagg tcgaggaccg gggcgaggag gccgagcccg
    24421 aaggcgaaga cgaacaggag ccccgagagc caccccgccg aacccggacg cccgccgagt
    24481 cccctgagac ccgtcgagcc ggtgcgccgt agctgcaaga gcatccttcc gccgacggcc
    24541 agcgtcaggt agagcgcgta cagcgcgagc gccagtaccg ccactacccg aggaaggtct
    24601 ggtacagcaa gaagacgttg agggagacga tcagggcgac cacgaccatc gccacggcgg
    24661 tagtaaggcg gtggttcacc agcgtcccca tcaggtcgcg gttgcggcag aacatcagga
    24721 gcgggatgag cgcgaagggg atgccgaagg agagcacaac ctggctgatg accaacgagc
    24781 ggctcgggtt gatcccgacg gcgaggatga cgagcgccgg cgccatcgtg attagacgcc
    24841 gcacgagcag cggtatctgc cggttgatga aaccctgcat tacgacctgc ccgctcaagg
    24901 tgccgaccga ggagcttgag agcccgctgg cgagcagcgc caccccgaag aggatggagg
    24961 cggagttgcc ttcgagggcg cgcaactgct cgaacgccct gtctatgtcg gcgacgccgg
    25021 tcaggccgtt ggagtggaag agcgcggcgg ccatgatgag catgctggcg ttgatggtcc
    25081 cggcgattgc catggcgatc accacgtcca ccagctcgaa gcggaagatc ttcctcctct
    25141 cctcctccgt cctgccgacc acccggcgct gcgtgagggc cgagtgcagg tagatgacgt
    25201 gcggcatgac cgtggcgccc aggatgcccg ccgccaggag cacgctctcg gtcccggcga
    25261 actgcggcgt gaagagaccg gccagtatcc tgtccgcccg cggctcggcg tagaacatct
    25321 ggaaggcgaa ggccaccacg atgaccccga ccatcgcggc gatgaccgcc tccagcggcc
    25381 ggaagccctt ccgctgcagc tccagtatcc cgaacgccgc caccgccgta attagccccg
    25441 ccgggaagag cggtatgcca aagagcaggt tcagcgccag cgccgccccg atgaactccg
    25501 cgaggtccgt cgccatggcg atgacctccg cctgcaccca cagaaagaac gttaccggct
    25561 tcgggaaccg cgcccggcac acctccggca ggttcttccc cgtcgcgatc cccagcttcg
    25621 ccgacatgga ctgtatgagc atcgccatca ggttggaggt caggatcacc cacaaaagca
    25681 gatacccgaa ctgcgccccc ccggagatgt tggtggcgaa gttcccaggg tccacatacg
    25741 caaccgccgc gatgaacgcc ggacccagga acggtaagag cctgcgtatc ccccgccgat
    25801 ctccctttaa agactggtgc gccgcccgcg ccgtcgcagt ctcccccggc aacacaccct
    25861 ctacaccctc tataaactcc atcgaccggt ccgccatgag aacctcttct cgtccataaa
    25921 aatttagtct tgcctaatct tatattagtt aatagctaat cgtcaagctg ccgctatctt
    25981 gataaatatt tcgcatcgtc taatatgagt tccgtgatga ccaacctcac ggcacctcac
    26041 tctccgtcat cttcggtcgg ggattacctg aaggctgtgt gggagcttgc cggggatacc
    26101 ggcgaggcag cttcgacgaa ggacgtggcg gcgcggctct cggtctcgcc ggcatcggta
    26161 tcgaacatgt tcgcgcggtt gcaggagatg gggctggtcg gttacgagcg ttaccggggg
    26221 gccatgctga cacagagtgg gcgggtagag gcgctgcggc tggtgcggcg gcaccgtctg
    26281 atcgagacct tccttctggg gcacctcggg tattcctggg aggaggtcca cgaggaggcc
    26341 gagcgactgg agcacgcggt gtccgacggg ttcacggagc ggctggcgga gttcctcggg
    26401 catcctggac acgacccgca cggcgacccc atcccgaccg ccgacggcgc tctggagccg
    26461 gacgactcgt tccctctcgg tgagtcggag gcaaaagagc gggtccgcat atacaaggtc
    26521 agcgacgagg atgcgcgggt gctggactac ctggcggaac atgacctcgt gcccggaagg
    26581 ttactcgaag tcagggagat ccgggctcta gacggcgtcg ttaccgtgaa ggacgaggac
    26641 gggagggtcc attcgctagg agagccgctg gcgcgttccg tcttcgtccg ggacgagccg
    26701 tagagcggcg cccgagaaga tagtcatgga caacccgtct cccgagccag actatccccg
    26761 ataccggcta ggtcccggag agcaggtgtc gctggcggag atagaccccg accagaccga
    26821 gcactacaag agaaagaagg acgtgctcaa ggatctggag aagcagcggc gacgcataca
    26881 ggatctgcag gagcggctct acgcggagaa cgagcagggc ctgctcatcg tgctgcaggc
    26941 gatggatacc ggcggcaagg acggcaccat caagcacgtc ttcagcggcg tgaacccgca
    27001 gggttgtagg gtctcctcgt tcaagactcc ctccgccgag gaggcgaacc acgacttcct
    27061 gtggcgttac cacaagagcg ctccggcgaa gggccgcatc gggatcttca accgctccca
    27121 ttacgaggac gtgctggtcg tgcgcgtcaa ggacctcgtc cccgaggagg tctggcgcat
    27181 gcgctacgag cagataaacg acttcgagcg caacctgacc ctcaacggca tcaccgtgct
    27241 caagttcttc ctgcacatct ccagggccga gcagaaaaag cgtctgcagg cccgcctcga
    27301 caacccggac aaacgctgga agttctcctc caacgacata aaagagcgcg ccttctggga
    27361 cgactaccag cgggccttcg agggagccat ccagaagtgc tcgaccgagt atgctccctg
    27421 gtacgtcgtg ccggcgaaca agaaatggta ccggaacctc gtaatcgccc gcaccatcgc
    27481 cgacacgctg gaggcgatgg acccgcaatt tcctccagcc gaagaagggt tggagaaagt
    27541 aaggatcccg gactaaccgg cgttattcgg ccccgttacg ccgggcaaag aggccgcgag
    27601 ctttcattgt catgacggtc tcgccgcgct ggtttcgcac ctccgagtag aggtggaccg
    27661 tgccgcggtt cgggttgcgg gtggatggag ccgtgtccag gacggtgagg ctggcgcgca
    27721 gggtatcgcc gggacggacg ggcttcagcc agcgaagctc ctcgacgccg ggcgagccca
    27781 tgctggcgga gcgggagagc agcgcgtcca cgtagaggcg catgaagatc gccgccgtat
    27841 gccagccgct ggcgatcaga ccgccaaacg cggagtccct ggcggattcc tcatccacat
    27901 ggaacggctg cgggtcgtat tcgcgggcga aggcgacgat ctcctccctg tcgatagtcc
    27961 gctcaccgaa gtcgaagacc atccccggct cgaagtcctc gaagtagaga aagccgttct
    28021 ctcgggtctc ccgcaccgtc agccccgctg cttcctgcgc cgcaggctct cccagcttcc
    28081 gtccccgaac cagccgccgt ccacggagag ggtatggccg ttgacgtagc cgctctgctc
    28141 ggggtcggcg aggaaggcga cggcccgcgc gatgtcttcg gggcgggcga agcggcccat
    28201 cggggtgcgg ccctcgatat ccgcgtcggt gtagccaccg ccggcctggt cctcctcgtc
    28261 catctccgtc ttcacccagc ctggacagac ggcgttcacc cgcacgccgc gcccgcccca
    28321 ctcggcggcc agggtgcgcg taaggccgat caaaccgtgt ttgctcgcgt tgtacgccgc
    28381 gcggtccccg atgccgagca gccccgccac cgagctgata ttgacgatgc tcccctctcc
    28441 tcgctccagc accaccttcc cgaactcccg gcacgtcagg aacgggccgg tcaggttcac
    28501 ggagagcgtg cggttccagt cttcgaggct cgtctcctcg gcgggggtga tagagctgat
    28561 cccggcgttg ttcaccagca cgtctatccg cccgaactcg ctcatcatag cttcgaccat
    28621 cccgcgcacc gatgcctcgt ccgatacatc accggggacg gagagcgctt ctcctccgat
    28681 ctgctctatc tctcgcagtg tctcctcggg agcccgcagg tcgttggcgg ctatccgata
    28741 gccgcgttcg gcgagggtca tggctactgt ccggccgatg ccgcgtccgg ctccggttac
    28801 gagtgctaca cggttctccg gcactagatc tcctctctca cttgacggcc cagaggtacg
    28861 agaaggcgtc acgggagacc cgttccgcgc gctccaccag cgggcgcagg tgagactcta
    28921 acctctccaa ttcatgtggg atcagagcct cgttcatcgc ttctccaagc gacgggacct
    28981 tcgggttgcc ggtccatcgg atgaaacggt cccaaccacc cagtatcccc gacgggttgc
    29041 cgcggctgat gctgatctct agggtaaggt ggacctcgct gaaacccgca gcgtccgcga
    29101 agtcggcgag gtcccgctca tcgaaatcca tcatcggatc gccgggcggc tgaatacgtt
    29161 cgtagaccgc cctgactttc cgggccaggt cctggaccgg agtcacgtcg taacccatga
    29221 acaggtgttc gggctccggg taggtgaagc tgttgatcgg ctcggcgatt gagagccttc
    29281 cgccggactt gagcacccgg tagaactcct cgaaggcgcg ccgcttgtcc tcgacataga
    29341 tcaagaccga gcgagtggtc accgcgtcga cagactcgtc ctccagcgca cccaggtcat
    29401 cagccgatgc gcgaaggaac cggcaccggt cgagaacccc cgtatccccg gcgagctttt
    29461 tgctgtgatc caggaggtcc tgagagatgt cgccgaaaat tagccgtccg ttcttcccta
    29521 cgatgtccag ggcgccgaac gcgatgagac cgtctcccgc gccgacgtcc agcagagtct
    29581 cgccgccgga gagcccggca ttctccagca cccggtcgcg tatcgggaag aggaactcca
    29641 ggctagcttt ttgctgtacc ggatctccac cgtcccgtcg ctccagcagc cactccgccc
    29701 acctgtcccg aaccggctcg gtcatcccct agctctgtac cggcgtcctg ggctcctcgc
    29761 cccgcaacac cgcgacgagg ttctccgcag ccagagtcgc catcttattg cgcgtctcga
    29821 tggagccgct cccgatgtgc ggcgctaaca cgacgttctc aagctccagc agcttcggat
    29881 gcaccttcgg ctcctcctcg tacacgtcga gaccggcggc gaatattcgc cgctccgcaa
    29941 gagcttccgc aagcgcctcc tcgtccacga caggaccgcg ggaggtattg actagtacgg
    30001 cctcgggctt catcttctcc agttccgccg cgccgataag atggcgggtc tcgtccgtga
    30061 gcggtgtgtg gacagagacg aagtccgcag tctccagcag ctcgtcaagc tccacgtagc
    30121 gagcattcag ctccttctcc gcctcctcgt tgcgagagcg gttgtagtag atgacctcca
    30181 tatcgaagcc tctggcgcgg cgggcgacgg cctgcccgat gcgccccatc ccgataatgc
    30241 cgagcttctt gccccacacg tccggcccca tgaacagctt cggcccccac cactcccact
    30301 taccggcgcg caggacgcgc tccgactcgc cgagcctgcg cgccgccgcg agtagcagca
    30361 tgaaagccac gtcggcggtc gtctcgtcga gcacgccggg cgtgttggtg acgacgatgc
    30421 tccgctcctt cgccgcctcc aggtccacgt tgtcgtagcc gacggccagg ttggcgatga
    30481 ccttgagtcc gtctccggcg gcgtccatga actcggcgtc catcttctcg gtgacggtgg
    30541 agagaacgcc ggatgagttg cgggcggctt cgagcaattc gttacgctcg ggcggcgctt
    30601 cggagaggac ggtaacgtcg aagtcttcga ggaggcggag cccggcggcc gggatctctc
    30661 gggtaacgag caccttctcc ggcacggact cctcctctct accgattctc tactggcctt
    30721 ccttgaggag ctgtctgagt accgtctgca ggatgccgcc gttgcgcagg tactcgacct
    30781 cgaccggcga atcgacccgc gccttgacct tgaactccgt ttcgtcgccg tcgtcgctcg
    30841 tggccttgac ggtgagctcc gcgccggctt cgaggtcggc caagcccttt atgtcgaacg
    30901 attccttgcc ggtgaggccg agggaatccg cgctctcgcc ttcggcgtac tggagcggca
    30961 cgacgcccat gccgatcagg ttggagcggt ggatgcgctc gaagctctcc gcgataacgg
    31021 cctttatacc gagcaggaac gagcccttcg cggcccagtc tcgggacgag ccggaaccgt
    31081 actccttgcc cgcgaggacg agcagcggca cgccttcctc ctggtacttc aggctagcct
    31141 cgtagatcgt ggtctcctca ccgtccggga agtggacggt gtagccgccc tccttgccgc
    31201 ccgcgagctt attgcgcagc cggatgttgc cgaacgtccc gcgcaccatg acctcgtggt
    31261 tgccgcggcg g
//
LOCUS       DASSMB010000017         6276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_890315, whole genome shotgun sequence.
ACCESSION   DASSMB010000017 DASSMB010000000
VERSION     DASSMB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890315"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VFJ72_01390"
     CDS             <1..668
                     /locus_tag="VFJ72_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7478146.1"
                     /translation="WVCDNWNNVDEGIWETRGGRRDFVYSRLMCWVAIDRGLRLADKR
                     SFPADRDRWLKVRDEIYEEIMEKGWSPERQAFVQSYGDDTLDASNLIMPLVFFLSPSD
                     PRMLKTLDAINRPPKDGGLVSNSLVYRYDVQKSADGLTGEEGTFSLCTFWLVEALTRA
                     GRLEESRLIFEQMLGYANHLGLYAEEIGHRGEALGNFPQAFTHLTLISAAYNLDRALG
                     MKG"
     gene            673..1341
                     /locus_tag="VFJ72_01395"
     CDS             673..1341
                     /locus_tag="VFJ72_01395"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7478147.1"
                     /translation="MLSEVRQRAMGDWIDVSVALHGGMVHWPDNPPVRIERMLDIERG
                     DVANVSKISMGSHTGTHMDAPLHFIRDGKGLEEMPFGAAMGRARVIEIQDPESIKPDE
                     LRPHGIRKDERILFKTRNSVRGWAEDDAFDEDFVFISQAAARYLAEREVQTVGVDYLS
                     VGGFHQDGEETHVALLGAGIWVIEGLDLSEIEPGEYELLCLPIKVERSDGAPARAILR
                     RVRG"
     gene            1388..1786
                     /locus_tag="VFJ72_01400"
     CDS             1388..1786
                     /locus_tag="VFJ72_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7478148.1"
                     /translation="MPARTPEEVPRLFAAAFGAHDVEAALMLYEPGATLIAQPGHAVT
                     GTEAIREALGGLLALESEFVIEVKKAFQVGDVALLFSDWTLSAATPDGQHIEMGGRTA
                     DVVRRQPDGSWLLVIDDPFGADHGFAQDAA"
     gene            1832..3235
                     /locus_tag="VFJ72_01405"
     CDS             1832..3235
                     /locus_tag="VFJ72_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HET7478149.1"
                     /translation="MEHYDAVVIGAGQAGVPLSQELARAGRKTALIERVHVGGTCINE
                     GCTPTKTMVASAKAAYIDRRSADFGVRNGLVAVDMAEVRRRKQGIVDSFRNGSQQRIE
                     DTEGLDLIMGEARFTGPKALEVRMNDGEALELRADNIFINAGARPANPPIEGLDTVPA
                     LNSTSIMELDELPEHLLVLGGSYVGLELAQMFRRFGSQVTVVQRSGQLMGREDADIAE
                     TVAEILREDGIEVLLNTQTRRAEQDANGKIHLTVETLGGEHTLTGTHLLVAAGRPPNT
                     DTLNLETAGIETDKRNFIKANERLETSVEGVYALGDIKGGPAFTHISYDDFRIIKTNL
                     LEGGDATIADRSVPYTMFIDPQLGRIGLSEQEARDRGLNFRVAKIPMDYVARAIEMGE
                     TRGFMKAIVDADTDRILGCAILGVEGGEIMAMIQIAMMGRLPYTALRDAVFAHPTLAE
                     SLNTLFSTIEQRQGAAV"
     gene            3334..3930
                     /locus_tag="VFJ72_01410"
     CDS             3334..3930
                     /locus_tag="VFJ72_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET7478150.1"
                     /translation="MLRTLNLDGDGQADLVGHGGIYKAAYVYSIENYDYWKRELGRTD
                     FAFGQFGENFTVEGMLEDGIHVGDVFRVGGALVEVTQPRVPCYKLGIKMGLPRFVKMF
                     LESCRVGFYLRVLEEGEVGAGDVFERVRTDPERMTVREICHLLYFEPDNLREARRALN
                     IEALSPGWRQSFEERLTKTGTSIDPSEVAQREPNNCGP"
     gene            3959..5065
                     /locus_tag="VFJ72_01415"
     CDS             3959..5065
                     /locus_tag="VFJ72_01415"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7478151.1"
                     /translation="MKAVAVYPGKPGSIHLDDLPAPKVDDVPNGRGVLVEVLRVGVDG
                     TDKEINAAEYGAAPEGYDVLVTGHESFGRVLEVGPNVRDIRPGDYVVATVRRPGSSIY
                     DLIGTYDMTTDDTYFERGINLRHGYLTERYVDDPEYIVKVPEALKEVGVLLEPTSVVE
                     KGIEQAYEIQRRLKVWRPERAAVVGAGTLGLLATLALRLRGLEVTTLARTAPPTLNSK
                     LVEALGARYVSTRETPLLEAAEEYGPFDIIFEATGVSSVVFESMEALGRNGVLVLTGI
                     SGGDHTVEVPGDRIMLGFVLGNKVAVGSVNANRTYFERGVMDMALAEAEYPGWLGRLL
                     THRVQGLDNYEEMIRLLTEEKGAIKVFVEVNGHR"
     gene            5176..5580
                     /locus_tag="VFJ72_01420"
     CDS             5176..5580
                     /locus_tag="VFJ72_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478152.1"
                     /translation="MDGCAEVLVSAFNAEPWNEGWTLETARKDMRSTLDTPGFLGFVY
                     LEDGVRGFAAGNREQDAAHVVFYLSVLCVSPEFQRRGIGGTLMRRLKEALTEMGIRSI
                     YLLTDRGTPAETFYEKNGYRVSPKDILMFHEW"
     gene            5641..>6276
                     /locus_tag="VFJ72_01425"
     CDS             5641..>6276
                     /locus_tag="VFJ72_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7478153.1"
                     /translation="MMPEISLTGVLIVAAVAFLVPLGLGLAPALRVPSVVIEIVAGIV
                     IGPAVLGLVEVDEPLRFMALIGLAFLLFLAGMEIDLGGLLRGGALKSAGIGFLLSLAI
                     AFGIASLLGISGLIQAPLLVAIMLSATSLGIVVPVLADSGQSRSTLGQLIITGSSVAD
                     FGAVILLSLFFSGDSSSVGSTLLLIGTFVVLVAAIGIALVEIEHLGRLSTVL"
BASE COUNT         1202 a         1901 c         2129 g         1044 t
ORIGIN      
        1 actgggtctg cgacaactgg aataacgtcg atgaggggat ctgggagact cgcggcggcc
       61 gcagggactt cgtgtactcc cggctcatgt gctgggtcgc aatagaccgg ggcttgaggc
      121 tcgccgacaa gcgctcgttc ccggccgacc gcgacaggtg gctgaaggtt cgcgatgaga
      181 tctacgagga gatcatggag aagggctgga gcccggagcg ccaggcgttc gtccagtcct
      241 acggcgacga caccctggac gcctcgaacc tgatcatgcc gctcgtcttc ttcctctccc
      301 cgagcgaccc caggatgctc aagacgctcg acgccatcaa ccgcccgccg aaggacggag
      361 gactggtctc caacagcctc gtctaccgct acgacgtaca gaagtccgcg gacggcctca
      421 cgggcgagga agggaccttc agcctgtgca cgttctggct cgtcgaggcg ctcacgcggg
      481 cggggcggct ggaggaatcc cgcctcatct tcgagcagat gctcggctac gccaaccacc
      541 tcgggctgta cgccgaggag atcggacacc gcggcgaggc gctgggcaac ttcccgcagg
      601 ccttcacgca cctgaccctg atcagcgccg cctacaacct ggaccgcgcg ctcggtatga
      661 agggatagcc gtatgctctc cgaagtacga cagagggcga tgggcgactg gatcgacgtc
      721 tccgtcgccc tgcacggcgg catggtccac tggcccgaca acccgcccgt ccgcatagag
      781 cggatgctcg acatcgagcg cggggacgtg gcgaacgtct cgaagatctc gatgggctcg
      841 cacaccggca cgcacatgga cgccccgctg cacttcatcc gggacggcaa gggtcttgaa
      901 gagatgcctt tcggcgcggc catgggacgt gcgagggtca tcgagataca agaccccgag
      961 tccatcaaac ccgacgagct tcgcccgcac ggcatccgca aggacgagcg gatcctgttc
     1021 aagacacgta actcggtccg gggctgggct gaggacgacg ctttcgatga ggacttcgtc
     1081 tttatctcgc aggcggcggc gcgctacctg gcggagcgcg aggtccagac cgtcggggtg
     1141 gactacctct cggtgggcgg gtttcaccag gacggggagg agacgcacgt ggcgcttctc
     1201 ggggccggta tctgggtcat cgagggtctc gacctgtcgg agatagagcc cggagagtac
     1261 gagcttttat gtctgccgat caaggtcgag cggagcgacg gcgctccggc gcgcgcgatc
     1321 ctgagacggg tccggggttg atccggtgga caaggataag aaacaggaag aaataggagg
     1381 tattcaaatg cccgcacgca cacccgaaga agtgccccgg cttttcgccg cggccttcgg
     1441 cgcccacgat gtagaggccg cgttgatgct ctacgaaccc ggtgcgacct tgatagcgca
     1501 gccggggcat gccgtcacgg ggaccgaagc catccgcgag gccctcggcg gccttcttgc
     1561 cctggagtcg gagttcgtca tcgaggtcaa gaaagccttc caggtgggtg acgtcgcgct
     1621 gttgttctct gattggacct tatcggccgc cacacccgac ggccagcaca tcgagatggg
     1681 cggccgaacc gcggacgtgg tgcgccgtca gcccgatggg agttggctgc tcgtgatcga
     1741 cgaccccttc ggtgcggacc acggtttcgc ccaggacgcc gcgtgaagca gcgggccgga
     1801 gatcaaacgc acaagcagga ggtttatcgt gatggagcac tatgacgcgg tggtgatcgg
     1861 ggccggccag gccggagtcc cgctctcgca ggagctcgcc cgcgccggac gcaagacggc
     1921 actgatcgag cgcgtccacg taggcggcac ctgcatcaac gagggctgca ccccgaccaa
     1981 gacgatggtc gccagcgcga aagcggccta catcgaccgg cgctcggcgg acttcggcgt
     2041 gcgtaacggc ctggtggccg tggacatggc cgaggtacgg cggcgcaagc agggcatcgt
     2101 ggacagcttc cgcaacggca gccagcagcg catagaggac accgaaggtc ttgacctcat
     2161 catgggcgag gcccgattca ccggcccgaa ggcgctggaa gtacggatga acgacggcga
     2221 ggcgctggag ttgagagccg acaacatctt tatcaacgcc ggcgcccgac ccgccaaccc
     2281 gccgatagag gggctggaca ccgttcccgc cctgaactct acctcgatca tggagctgga
     2341 cgagctaccg gagcacctgc tggtcctcgg cggcagctac gtgggccttg agttggccca
     2401 gatgttccgg cgcttcggaa gtcaggttac ggtcgtgcag cgcagcggtc agctaatggg
     2461 ccgcgaggac gccgacatcg ccgagacggt tgcggagata ctgcgcgagg atggcatcga
     2521 ggtcctcctc aacacccaga cccgccgcgc agagcaagac gccaacggca agatccacct
     2581 gacggtggaa acgctcggcg gagaacacac gttgactggc acacacctcc tggtagcggc
     2641 cggacgcccg cccaacacag acaccctcaa cctggagact gccggtatag agaccgacaa
     2701 gcgtaacttc atcaaggcga acgagcggct ggagaccagc gtggagggcg tctacgcgct
     2761 tggggatatc aagggaggcc cggcgtttac gcacatctcc tacgacgact tccgcatcat
     2821 caagacgaac ctgctggaag gcggggacgc caccatcgcg gaccgctcgg ttccgtacac
     2881 gatgttcata gacccgcagc taggacggat cgggttgagc gagcaggagg ccagggaccg
     2941 gggcctaaac ttccgcgtgg cgaagatccc gatggattac gtcgcaaggg cgatagagat
     3001 gggcgagacg cgcggcttca tgaaggccat cgtggacgcc gatacggacc ggatcctggg
     3061 ctgcgcgatc ctcggcgtgg agggcggcga gatcatggcg atgatccaga tagccatgat
     3121 gggcagattg ccctacacgg ccctccggga cgccgtcttc gcccacccga cactcgcgga
     3181 gtcgctcaac accctgttct cgaccataga gcagcgacag ggagccgcgg tgtgaagctg
     3241 ctatcggtaa acgtatcgcc gcccacggaa gttccgtaca ggggcaagac ggtaacgacc
     3301 ggcatcttca aagagccggt cggaggccgg gttatgctcc ggacgttgaa cctcgacggg
     3361 gacgggcagg cggatctcgt aggtcacggt gggatctaca aggcggccta tgtgtactct
     3421 atcgagaact acgactactg gaagcgtgag ctcggtcgaa cggatttcgc cttcggacag
     3481 ttcggggaga atttcaccgt cgagggaatg ctggaagatg ggatccacgt cggcgacgtt
     3541 ttccgcgtgg gcggcgcgct cgtcgaagtc acacagccgc gggttccttg ctacaagctc
     3601 ggcataaaga tgggcctgcc ccggttcgtg aagatgttcc tggaaagctg ccgggtaggc
     3661 ttctacctgc gcgtcctgga agaaggcgag gtcggggcgg gcgatgtctt tgaacgcgtc
     3721 aggaccgatc ccgaacgaat gacggttcgg gagatctgcc acctgctcta cttcgagccc
     3781 gacaacctga gagaggcgag gagagcgctt aacatcgagg cgctctcccc gggatggcgg
     3841 caatcgttcg aggagagatt gaccaaaacc ggaacctcga ttgatcccag tgaagtagcc
     3901 caaagagagc cgaataactg tggaccatga aggcagtatg accatgagga ggaacacgat
     3961 gaaagcagta gcagtctatc ccggcaaacc cggcagcatc cacctggacg acctgccggc
     4021 gccaaaggtc gatgacgtcc cgaacgggcg cggcgtgctc gtcgaggtgt tgcgcgtcgg
     4081 cgtggatggg acggacaagg agatcaacgc cgccgagtac ggcgccgctc ccgaaggcta
     4141 cgacgtgctc gtaaccggcc acgagtcatt cgggcgcgtg ctagaggtcg gccccaacgt
     4201 gcgggacata agacccggag actacgtcgt ggcgaccgtc cggcgtcctg gaagcagcat
     4261 ctacgacctc atcggcacct acgacatgac caccgacgac acgtacttcg agcgcggcat
     4321 caacctgcgg cacggctacc tgaccgagcg ttacgtggat gacccggagt acatagtcaa
     4381 ggtccccgag gcgctgaaag aggtcggcgt gttgcttgag ccgacgagcg tcgtggagaa
     4441 ggggatcgag caggcctacg agatacaacg ccgcctgaaa gtctggcgtc cggagcgcgc
     4501 tgcggtggtc ggagcgggta ctctggggct cctggctacg ctcgcgctgc gcctgcgcgg
     4561 cctggaggtc acgacgctgg cccgcaccgc gccgcccacg ctcaacagca agctggtgga
     4621 ggcgcttggc gcgcggtacg tgagcacgcg cgagacaccg ctcctggagg ccgccgagga
     4681 gtacggcccg ttcgacatca tcttcgaggc gacgggcgtg agcagcgtcg tgttcgagag
     4741 catggaggcg ctgggccgca acggcgtgct ggtgctgacg ggcatcagcg gcggcgatca
     4801 cacggtagag gtgccgggcg accgcatcat gctcggcttc gtgctcggca acaaggtggc
     4861 cgtcggttcg gtgaacgcca accgcaccta cttcgagcgc ggcgtcatgg acatggcgct
     4921 ggccgaagcg gagtatccgg ggtggctggg gcgtttgctc acccaccggg tgcagggcct
     4981 cgacaactac gaggagatga tccggctcct caccgaggag aagggcgcga tcaaggtctt
     5041 cgtcgaggtg aacggccacc gctgagcacg aagctggaga gagaccggga gttcgatgtg
     5101 agagcagagt caagtagcac gggacctcca ggtgaagggg cggccggaag gatctccgcg
     5161 ctcacggagg agcatttgga cggatgcgcc gaagtcctgg tctccgcctt caacgccgag
     5221 ccctggaacg agggctggac cctggagacc gcccgcaagg acatgcgctc gacgctggac
     5281 acgccgggct tcctcggctt cgtgtacctc gaagacggcg tgagagggtt cgccgccggc
     5341 aaccgcgagc aggacgccgc ccacgtggtc ttctacctca gcgtcctgtg cgtcagcccc
     5401 gagtttcaga ggaggggtat aggcggcacg ctaatgcggc gcctgaagga ggcgttgacg
     5461 gagatgggca ttcgttcgat ctacctgctc accgacaggg ggacgccggc ggagaccttc
     5521 tacgagaaga acggctacag ggtcagcccg aaagacatcc tgatgttcca cgaatggtaa
     5581 tcgccccgct atgtaaacca gggataccgt cttccaggta tcccggacag gaagcttatt
     5641 atgatgccag agatctccct cacaggtgtg ctcatcgtcg ctgcggtcgc gttcctggtg
     5701 ccgctcggtc tgggtctcgc gcccgccctg cgagttcctt cggtcgttat cgagatcgtg
     5761 gccggcatcg tgatcggtcc ggccgtcctc gggctcgtgg aggtggacga gccgttgcgg
     5821 ttcatggccc tgatcgggct ggcgttcctc ctgttcctcg cggggatgga gatagacctc
     5881 ggcggactgc tgagaggcgg ggcgctgaag agcgccggca tcgggttctt gctatcgctg
     5941 gcgattgcgt tcggcatcgc gtctttgctc gggatctccg gtctcataca ggctccgctg
     6001 ttggtggcga tcatgctgtc ggctacctcg ctgggcatcg tggtccccgt gctcgccgat
     6061 tccgggcaga gccggtccac gctgggacag ttgatcatca cgggctcgtc agtcgcggat
     6121 tttggggcgg tgatcctact ctcgctattc ttctcaggag actcgagcag cgttggctcg
     6181 acgctccttt tgatcgggac gttcgtcgtg ctggtggccg ccatagggat cgcgctcgtc
     6241 gagatcgaac acctgggaag gctgagcacg gtcctg
//
LOCUS       DASSMB010000018        27626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_763795, whole genome shotgun sequence.
ACCESSION   DASSMB010000018 DASSMB010000000
VERSION     DASSMB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 27626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27626
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763795"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..877
                     /locus_tag="VFJ72_01430"
     CDS             50..877
                     /locus_tag="VFJ72_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET7478154.1"
                     /translation="MVPDSAPGEREALSLTRCVAYVLDVDPSEVPGDGGVGLRQWLAL
                     WNLGLVPVESPEDFAWPGRFLGRRRGSRTWAVLFGVPPGVVFDPAPDAAGSEDVDAAF
                     VLAAHDPGRQIGLDQPVRPSKTGVVELISVSEEAEGPMLSVTSAEALAGRGLRGDRYA
                     RGAGTFSDPDGRGYDLTLVEAEALEELAASGVEIEPAEARRNLVVRSIALDDLIGKRF
                     RVGGVECFGQRRCEPCAHLEKLTRPGVLRGLVHRGGLRADVLSDGEIRTGDRVEALS"
     gene            complement(874..1503)
                     /locus_tag="VFJ72_01435"
     CDS             complement(874..1503)
                     /locus_tag="VFJ72_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET7478155.1"
                     /translation="MPDATTFGLFLTAALVIAVTPGPGIFYVLTRSLKGGRGEGFAST
                     FGNSLGGLVHVLGAALGLSAILMASATAFTVVKLAGAAYLIYLGIRTLLGSDEAPAEI
                     SIEPGGHRDAFYQGIVAEVLNPKTALFLLAFLPQFVNPQGSVAFQILLLGCISVFLNT
                     VADLVVAFFAGPLGQRMRESVRLRRGQRLLSGGVLIGLGAYVALSGEKR"
     gene            complement(1537..1767)
                     /locus_tag="VFJ72_01440"
     CDS             complement(1537..1767)
                     /locus_tag="VFJ72_01440"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET7478156.1"
                     /translation="MDRREKIHPDSGSLSGQPVARGTRLSVDFLLDLLASGWSEEQLL
                     DSYPNLKRDSLRAVYAFAAQPARGERAFQPRR"
     gene            complement(1780..2781)
                     /locus_tag="VFJ72_01445"
     CDS             complement(1780..2781)
                     /locus_tag="VFJ72_01445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET7478157.1"
                     /translation="MQTTGTVGYAAMFEQFHPTDLLKWCKMAEENGFTSVMASDHFHP
                     WTPDQGQSAFVWSWLGALGATTSLRFGTGVTPPGFRYHPAIIAQAAATLEAMYPGRFW
                     LGLGAGEALNEHIVGDYWPEAPTRLRILMEAIEVIRRLFSGKVVKYSGEHITLESAKL
                     YTMPDTPPPIYVATAGPLQSRRTGEFCDGIITVGAADEKINMLMEKFEEGARRADKDP
                     SEMPRMIQLHVSWAENQEEAEQQAVKEWPNGGMPFPKGDIRNPEDFAAMAKQVSVENF
                     KNRVLMSADLDEHLEHIQHYVDLGFDEVYVHNCGRNQEEFIRAYGERVIPKLRWPEE"
     gene            complement(2857..4035)
                     /locus_tag="VFJ72_01450"
     CDS             complement(2857..4035)
                     /locus_tag="VFJ72_01450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478158.1"
                     /translation="MKVRGYQVEDREALARLAKTAFGDPGGSGYWERYYDPENARLDP
                     EQVHIIEEDGEPRATATVLPLEVFLGGKTVPMGGVAAVTTHPAYRRRGYAGDLMRAVL
                     RDMRERETRLSMLWPFSHAFYRAHGWELAGETISYTFKPTDLPTSPEQKHVRAYREED
                     LSRMTALFDAEASGHDLCVRRNEVRWRQFLGRKEQEVAVYEREGKVGGYAIYNMTDWD
                     RDKYPYRTLNVSELIADTLEARKALVSFMAAQDPLVFQVKHSTPRGEPLHPFLRSSHV
                     RMEIETEFMLRLVDVEGALKFKSLVGEPLVLEVSDDGIPENAGEYTVGNGEVTGEAEA
                     ENRVSLDVRQLAQLYAGYLPARQLARYGLVAASSSEALDLLEAHFPVGDPWVSEPDHF
                     "
     gene            complement(4091..4687)
                     /locus_tag="VFJ72_01455"
     CDS             complement(4091..4687)
                     /locus_tag="VFJ72_01455"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7478159.1"
                     /translation="MTTLGYAILGLLAQEALSGYDLTQRMKGRVGNFWSARHSQIYPE
                     LARLEGDGYVTHSVVAQQDRPDKKVYEITAAGLETLKEWVTEPPSPRPTRDELVLKAY
                     CVWLADPEKAARLFREEELRHEEQLARYEKTRDWMESEHGEEVNRPDSPWFAGYASLR
                     RGIGYETEYAQWCRWVADSIEGKAEPSGRRKKGRDSKS"
     gene            4793..5299
                     /locus_tag="VFJ72_01460"
     CDS             4793..5299
                     /locus_tag="VFJ72_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478160.1"
                     /translation="MCGRSYAVRGAGFASGRRRLFGLDAEKMSPEVRVAVALSVLGPV
                     ALSGIFLVVFVPSLWWIFTTYFWVSFPALGMLGSGIAGLSKARPARVSEEDRERELLT
                     SLARHGEVSAAAVAAETSMTVSEADRKLRELAEGGHLEVRVRGGAIFYALWEANPVES
                     GSEIEGVR"
     gene            5317..5781
                     /locus_tag="VFJ72_01465"
     CDS             5317..5781
                     /locus_tag="VFJ72_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478161.1"
                     /translation="MTVSRASEEKTRRRLLIFAGAATVFSVLHHTDHVIRGNHSGWPF
                     VAEITPFTFSLLIYALILPGIYLTARAHKIAGYHLFVAIVGLALLGFVHFAPLGDYEA
                     PISDIYMAYDSPVVGLAAHFVLAGLIACVALLAVTALGALRSRVRILDSERG"
     gene            5785..6057
                     /locus_tag="VFJ72_01470"
     CDS             5785..6057
                     /locus_tag="VFJ72_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478162.1"
                     /translation="MKTRKVIGTKQPPFRAQVAFARAATGLGWMSVGALAVGATAVGA
                     VAIGALAIRTLAVKRGKIERLSIEELEVGRLRVRELIVESEQGEED"
     gene            6061..6567
                     /locus_tag="VFJ72_01475"
     CDS             6061..6567
                     /locus_tag="VFJ72_01475"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HET7478163.1"
                     /translation="MARILNGRYTARTDEPFVVFIIGMRINNLFAVNKWLPTARAMGP
                     MLKELYQHPEKGFLGAEFFMYWRGPALVQYWRSFEDLERFARNPDDPHMPAWQRFNRS
                     VGKDGSVGIFHETYIVERSNYEAIYSNMPVFGLAKATEHVRAVGGRETARRRLMRGDN
                     EPAVPSPE"
     gene            complement(6580..7143)
                     /locus_tag="VFJ72_01480"
     CDS             complement(6580..7143)
                     /locus_tag="VFJ72_01480"
                     /inference="COORDINATES: protein motif:HMM:NF022215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HET7478164.1"
                     /translation="MMRKTLPWLAIIAVLAGAAYQLRSQGRLWLCSCDYLLLWSGDAW
                     SSDNSQHLLDPYSFTHVLHGFIFYGLLALIIPRLPVIWRLWLAVSIEAVWEVVENSEY
                     VIRRYREGTAALGYHGDTIVNSLSDIVLCGLGFALARRLGFRRTLLVLVATEVTLAFW
                     IRDGLLLNIIMLIYPLDAIREWQAAGH"
     gene            complement(7204..7614)
                     /locus_tag="VFJ72_01485"
     CDS             complement(7204..7614)
                     /locus_tag="VFJ72_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478165.1"
                     /translation="MSLLTEALIVSGVAMDLVGAGVLSHAHNAESIAEIRAEIGAEKS
                     AVGENDAIATHAQLLAEKRAGFFILTVGLVLYLSGLVIKSPQGTGTMVVLATGVVIAS
                     VIGTVIWTRVVGNKVKEQARKAERHGDEDELPEQ"
     gene            7948..8916
                     /locus_tag="VFJ72_01490"
     CDS             7948..8916
                     /locus_tag="VFJ72_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET7478166.1"
                     /translation="MLLISGMWLFALVGGCVPSADTNSPTTGSNGNTARSAERNSASS
                     PLEDKPWRLETIVRNGEPEQVTETLRPAIAYFKSGKVGGRAACNYYGGPYEVDGKKLA
                     IGALMGTSVGCGGHKGEEEKALRRGMQSAESYTVEGDRLTLAFPDGKLIFQALPDVRG
                     DEQAVYAAVLEEWDGGPGPIVIVNKNRIGPAGRNLDDAVDRIRRENAARPGYKPILDA
                     ETLSDFRDKNTGTLDMSRVVDLEVPTVFVSEAEIEALFTDGEEAGWSRFKKTYPGARE
                     IISLSRVGFNGNGDQSLVYIGVRAKDYEEGHYDLMNYHSGLWVSTS"
     gene            complement(8957..10492)
                     /locus_tag="VFJ72_01495"
     CDS             complement(8957..10492)
                     /locus_tag="VFJ72_01495"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7478167.1"
                     /translation="MSFPLIPDGPLGRAASIASRHPSYRVEGANGTARVNLGLDLPAD
                     WRPLDDLSNLLRGEKRVDYTADGVALSGEELFDGLRCFLRKQRSGASSREWCTPKSLE
                     GKQLFPCKQIHVYDNDHLTKSSWYGFGEMGEDGVFKVDKEAITDRVLSDLGPCVRCPI
                     LDLDTTAELVARLPEQIDPSREDGWRYREDGPLTNWKVSKTHEGIEEASSAEEKQTLL
                     ERLAQSVRSGGVNVAAASGAAPGSRNVPQTRYEDVGGMRETIALVRESVELPITHPEI
                     FKKLGIRPHKGILFYGPPGTGKTLLARAVAHESGAHFIAVSGPEILNKFWGQSEAKLR
                     SIFSEARTKSPAIILFDEIDSFASSRDMMSESFEATLVSQLLSLMDGMNDLGRVCVIA
                     TTNRPSALDSALRRPGRFDHEIEIGLPDTEARLHILKIHTKNIPTDPDLDLKQIAQIT
                     GSYSGADLEALCREAALVCMRRSINLRDFEKRIASHQLSALSVTTYDFRTAMKRVGPS
                     IRR"
     gene            11100..12296
                     /locus_tag="VFJ72_01500"
     CDS             11100..12296
                     /locus_tag="VFJ72_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478168.1"
                     /translation="MTKETATVYGYTYGQASGNRVVEGRGRLPEAKPIDVSLGGTPTW
                     VVGVPLEDDTAWVVAYGDGRVDTFRLDGESGKVEPWLTSPDKLPPGAPPAVAVQGERL
                     DLITAPGKRSSSLTHPIRGEAGLLGITAGGKLFTEPGEGPRLSALPDARIVQSSSGAA
                     AVLAAPTARYDHGILGDRLEAGEIGVLKPGGGGYRLTTEVQPESGGVFEALAPLWFRP
                     GAGEDELLAVTESAERRGSRISVYSPDGGLVAAGPFINAPQQWRHLIAAGPFGPDGQI
                     EIAAMRTPHIGGTTEFYRLDRKSGELELAATGPGYPSHTIYSRDLDAARAGDLDGDGS
                     WELLVPNTAYNGLVALRHTKNGVEKVWSLPATNSLGTNIAAVTDSGGRIAVAVGTLDG
                     NLRIWR"
     gene            12360..13334
                     /locus_tag="VFJ72_01505"
     CDS             12360..13334
                     /locus_tag="VFJ72_01505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7478169.1"
                     /translation="MAVDFGVFVPQGWRMDLVGIEGPFEQYETMTRVAKAADESGAYD
                     SIWVYDHFHTVPEPTMNTVFEAWTISAGLARDTKNVKIGQMVTCNGYRNPALLAKIAS
                     TVDVMSNGRLLCGLGAGWYEHEWRAYGYGFPEVPVRMRMFKEACEIVHRMFTEDHPTF
                     DGEFYTIDQPINEPKGVSDPHPPIWIGGGGEKVTLKLVAKWGDATNVNGDIETLKHKF
                     EVLKGHCENEGRDYDEITRSTSVNVHLLEDGQDPEEATREARGEMSYEEYSEGPHAFM
                     VGTPEQISERLQPMVDAGVEYFITYLPGVAHDLSPVERFANEVIPNFR"
     gene            complement(13347..13712)
                     /locus_tag="VFJ72_01510"
     CDS             complement(13347..13712)
                     /locus_tag="VFJ72_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7478170.1"
                     /translation="MPRIEVEGVGAFDVEEGKRLVLAIEEDAGVDILHRCGSYAKCTT
                     CRIEYLEGEPEKMTKAELDVLEKRDLLGQVRLSCQAACDHDMKVRVLMTVTSTGLDGP
                     GPHPEAQITPEPEWVEKPA"
     gene            14034..14396
                     /locus_tag="VFJ72_01515"
     CDS             14034..14396
                     /locus_tag="VFJ72_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HET7478171.1"
                     /translation="MDYRPPSVRVEKPWGRFEQYTHNLPCTVKIITVAPGGTLSRQYH
                     HQRDELWVMLDPGARVELDDKVLHPDPEEKLFIPRETVHRLSSTGDKPVRILEISFGE
                     FDEEDIVRLEDVYGRVEP"
     gene            14537..15340
                     /locus_tag="VFJ72_01520"
     CDS             14537..15340
                     /locus_tag="VFJ72_01520"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HET7478172.1"
                     /translation="MPWLLVLGGVLLLFLLLGAVILLWRSFSDSLAGTSVARDSIDSG
                     PKPGIKLTNGAGQIRIEGVEDLGSVEYEVTRHALGPDPATAKERASKVTVNVARDGGD
                     FVIETDGGRNTGADYAMRVPAGSSVDIESGAGDVEVSKISGDVSVDAGAGDVSVREVK
                     GSVKVKAQAGDVEVSKMQTDTGGADIEVASGDVTLQDLVAGTLETSVEAGDVTLSGRF
                     SGGGRVSVKTGDIIAQLPSEDTRDLTLQTQVGQVVREDAGGPGKKPEKS"
     gene            15340..15930
                     /locus_tag="VFJ72_01525"
     CDS             15340..15930
                     /locus_tag="VFJ72_01525"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET7478173.1"
                     /translation="MFRRSGQQSMGYSPESFREALKEVWFAPGRFFKRLDPEGGPIRP
                     TIFASLVLYLNLLLDAVLQAFWLGEYNLSLVYAPFVGLLVALVLAPLLISGLTALTLV
                     ILDGAPSRRNFGPVYRAFGYASGIGIVVWIPYGPLLAIPYGAAVATIAVKETLGLGWR
                     RAAAAALIPLGAVFLIVLLLVGPTEAYGFLINPAGS"
     gene            complement(15938..17116)
                     /locus_tag="VFJ72_01530"
     CDS             complement(15938..17116)
                     /locus_tag="VFJ72_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7478174.1"
                     /translation="MDVTQRFDGLQREIEKDFGEKIVTVRRDIHREPELGFDTVKTAE
                     KVLAALEGLPLEIQTGIAENGIVATLRGGGDGPTIGLRADMDALPIHEETGLPFASET
                     DGKMHACGHDGHTSMLVGAAHALSGMRDRLNGTVKFFFQPAEEGGGGGKVMVEEGAAD
                     GVDSVFALHLWPGLPVGMVATKAGPIMAAADGFEMRIKGSGGHGAMPHLTADAVVIAA
                     QVVTALQTVVSREVDPVEPAVLTVGEIGAGTAFNIIPETARIGGTVRTLNAELRRRIP
                     ERMEELARGVAGGMRGDVELDYRFSYPVTMNDPGAASRALGVAENLFGAESVLELPNP
                     SMGGEDFAFFLEKIPGAFIWLGIGEDASGLHTPQFAFDEDVLPRGSALLAALALEALS
                     "
     gene            complement(17208..18158)
                     /gene="gluQRS"
                     /locus_tag="VFJ72_01535"
     CDS             complement(17208..18158)
                     /gene="gluQRS"
                     /locus_tag="VFJ72_01535"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HET7478175.1"
                     /translation="MYGTEQTRVGNVDNADGRFAPSPTGPLHVGNLRTALLAWLFARS
                     SGARFLVRVEDLDPSRVRPGMEETQLSDLRALGLDWDEPVVRQSERMELYTDAIARLE
                     ADGLLYPCYCTRAEIRASASAPHGISAGDRYPGTCRELTAAQRAKREAAGRPPALRVR
                     SNEARVSFEDRLLGFREDEVDDFVVRRNDGAPAYQLAVVVDDAAQGIGEVVRGADLLD
                     STPRQVLLARLLGLPVPRHVHVSLVLGPDGQRLAKRHGAVTLADRAAEGESPEDVLGW
                     MARSLGLAGPGERPTINDMLTRFDPDRLPQEPTVWSPGSG"
     gene            complement(18311..18727)
                     /locus_tag="VFJ72_01540"
     CDS             complement(18311..18727)
                     /locus_tag="VFJ72_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7478176.1"
                     /translation="MLVALWLDVDVRSGVPIYVQIVGQVRRAVEVGGLQAGERLPTVR
                     ALAEELTVAPNTVVKAYNELQRMGLIESKPGVGTVVAQDVGETAREQRIEALNERLGE
                     LVRDAVGLGISEDDLWGRLDAEFDRFYGRSASREDA"
     gene            complement(18829..19575)
                     /gene="yqeC"
                     /locus_tag="VFJ72_01545"
     CDS             complement(18829..19575)
                     /gene="yqeC"
                     /locus_tag="VFJ72_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HET7478177.1"
                     /translation="MNLYLALGITSGDVVAFAGAGGKTSAIREISAGLTRDGMRVLVA
                     PTTKMMVSEAEQIGPLVTSEDPEELRARVREALTDANAVVAGSSLISKGRVGGIEPNL
                     FGTLADLADVVLVEADGARHRLLKGTADHEPALPDVTTLVVALGNIRALGSPVDEEHV
                     HRPQIFSDLTGISLGQSITARAFARALAKGSLGSIPYEAQPAILITGVEPGRSMSDAS
                     IITREIWRMGIKKVVLSSLPQESPGRVWIP"
     gene            19780..20229
                     /locus_tag="VFJ72_01550"
     CDS             19780..20229
                     /locus_tag="VFJ72_01550"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7478178.1"
                     /translation="MAQGRERVAEFGRELREGPEPSDRSIKEIVDALRPQLQELASKQ
                     VELAKTELAPVGRKAGIATGLLVVGAVFLHLFLVFISVTGIYLLNEVAGLSLWLSALI
                     ISGILLVIGGILAGAGAGQLRGLDPKPHRTIRTMQQNIEWLKGQFRS"
     gene            20226..20570
                     /locus_tag="VFJ72_01555"
     CDS             20226..20570
                     /locus_tag="VFJ72_01555"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HET7478179.1"
                     /translation="MSEIPERIEREMFEIRSRMAPDVRDLKQHTNPQVVGKQVSAKLK
                     ERIKGAVTGFARSLSNSAKRQVKMAREAGRKRNPAPFTDAVKSDPRPLVLLAFALTVV
                     LMTARKISNVRN"
     gene            20640..21836
                     /gene="larC"
                     /locus_tag="VFJ72_01560"
     CDS             20640..21836
                     /gene="larC"
                     /locus_tag="VFJ72_01560"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET7478180.1"
                     /translation="MTHVHFQPIGGAAGDMMLASLIHAGASPDLISGGLDKLGVPFEL
                     HVERVEVNSMAALHLTVTHPEEHTHRAFRDIRALIESADLPQRVAERTSTAFRHLAIA
                     EGAVHGKDPEEVTFHEVGAIDSIIDIVGSCVALELLGAGTVSCGPLPMGGGLVDAAHG
                     KLPIPGPATLEVLKGSRVRWTEERREMTTPTGAALMRALTGGKFTDAAPPMTLGSIGY
                     GAGRARFETVPNLLRAVVGEIEGSGSGVEVLEANVDDAPGELLGASVESLLHAGALDA
                     WLEPILMKKGRGAYKVCALARTEDRDRLARTMMLETGTLGVRHHAVGRTVAERRIVQV
                     DLPYGPCRVKVGSLDGEDFAVAPEYEDAVRLASASGLSLPQVYSDARRIFEASGGNRI
                     KKKDRG"
     gene            complement(21870..22073)
                     /locus_tag="VFJ72_01565"
     CDS             complement(21870..22073)
                     /locus_tag="VFJ72_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7478181.1"
                     /translation="MAQGTVKWFNDEKGYGFISPDDGGEDLFVHYTGIAGNGFKSLEE
                     GTKVSYEPTQGRKGMQAENVSPL"
     gene            22273..23250
                     /locus_tag="VFJ72_01570"
     CDS             22273..23250
                     /locus_tag="VFJ72_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478182.1"
                     /translation="MKETDTPKHPGASYSITGGYAALKDGAAIVDHPDRALLRLSGKD
                     PRGMLNAILSNDVPADESRGVYAMLLNPKGRILSGLRVLKSNEEILIEAEAGGAASAK
                     ETLGRYAPFSRVKVEDLSDSWSVMGIYGHGAETLLDCPALAEHETAEIEVDGATLLTA
                     GVLTPAPGYDLIGPSEVVAAARDRLVARGATPADHSAYETVRIETGVPRYGADITPDN
                     FPGETGLLERAVNFHKGCYPGQETVARMHYRGHPNKTLYRLAIEGHPPELGENIIQAG
                     KVVGFITSVAPLPVEDRILALGYLSRTADIEEPLQTREAGISPLGPVGS"
     gene            complement(23298..25397)
                     /locus_tag="VFJ72_01575"
     CDS             complement(23298..25397)
                     /locus_tag="VFJ72_01575"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET7478183.1"
                     /translation="MSHIPEHHSPARIKSVMREMTIHASRVRLAESPRIDDDAETKRG
                     VRLPHMPGLDGLRAFAVIAVLLYHADLGWIPGGFLGVEVFFVISGYLITALLLAEWRQ
                     RGRIDLKGFWYRRARRLLPALYLLLVVTLAFAVVFLPGEVAGLRSDALAAFGYVTNWY
                     LVIGHESYFEAMGRPSLLKHLWSLAVEEQFYLLWPLLFAAGMGVGAMRYRRHRMIFFI
                     IVGAFASILLMAVLYKPGVDPSRIYYGTDTRASGLLIGAALAFLWEPGRFYTRAAIRR
                     AFEQGMGWVFPLLLDLAGLGALGVLVWFCLRITEFQPFLYRGGFACVGLATAVVIMVT
                     VHPRTYLGGALLGRRTLRWVGVRSYGIYLWHWPVYMITRPDIDVPIHGWPLLVVRLAA
                     AVALADLSYRFVETPIRKGALERSWRALREAQGGRRWQLGVRWAGAGVVSVAVSVMLS
                     VAVVQAKPPEKPSYLDMKAIHTSDKTPQKPSTQKAVDTQSEPRTHPQKKTGDHEKAAG
                     TRKTGKETANPASSSGTAAKEKEKTAPDKAEAAPVGRVTAIGDSVMLGAAAYLDQSVG
                     NLDTIDAQVGLQMAAATDILRARAAAGQIGDTVIVHIGNNGPISSAEFDQMMQALQSA
                     NRVIIVNLKVPRSWEQPNNAVLAEGVKKYPKAELVDWYGASVNHPEFFWDDGLHLRPE
                     GGQFYANLIAEQLKTGE"
     gene            26072..26818
                     /gene="sufC"
                     /locus_tag="VFJ72_01580"
     CDS             26072..26818
                     /gene="sufC"
                     /locus_tag="VFJ72_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7478184.1"
                     /translation="MLKIESLRAEIDGQEILKGLDLDVGKGEIHAIMGPNGSGKSTLS
                     NVLMGHPRYEVTGGSVTFMGEDIFELEADERAKLGMFLAFQYPSEVPGVSVANFLRTA
                     VNSVREEDLSPMEMYRLIQEKMKIMQMDPKFAERYLNEGFSGGEKKRNEILQMLMLEP
                     KLAIMDETDSGLDIDALQVVAKGVNELRGPDFSAVIITHYQRILRYIEPDHVHVMLDG
                     RIVTSGGKELALALEDKGYDWVRQEFGSAA"
     gene            26833..>27626
                     /gene="sufB"
                     /locus_tag="VFJ72_01585"
     CDS             26833..>27626
                     /gene="sufB"
                     /locus_tag="VFJ72_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7478185.1"
                     /translation="MPEDKTIQELGLDEYKFGFRDPEEYFFKTPRKGIDPEIVAMISK
                     HKNEPEWMLEFRLKALESFFEKPTPTWGGDLSEMDFDEIYYYIRPMENQGRSWDDVPD
                     DIKNTFEKLGIPQAERESLAGVGAQYESEVVYHSLKEEWEKSGVIFMDMDGGLREHED
                     IVREYFGTIIPPDDNKFAALNSAVWSGGSFVYVPPGVHIDIPLQAYFRINAENMGQFE
                     RTLIIADEGSYVHYIEGCTAPTYTSNSLHSAVVELIAKPNARIRYST"
BASE COUNT         5154 a         8738 c         8679 g         5055 t
ORIGIN      
        1 gtaaagttac tttaagtccc tggaaatcaa tggaactgga ggaacagaga tggttccaga
       61 ctctgcgccg ggcgagcgcg aggccctatc gctcacccgc tgcgtcgcct acgtgctgga
      121 cgtagacccg tcggaggtcc ccggcgacgg aggggtcggt ctgcgccagt ggctcgccct
      181 ctggaacctc gggctggtcc ccgtcgagtc gccggaggac ttcgcgtggc cggggcgctt
      241 tctcggtcgc cggcgtggtt cgcgcacctg ggccgtgctg ttcggcgtgc cgccgggcgt
      301 cgtgttcgat cccgcgccgg acgcagcggg gtcggaggac gtagacgcgg cgttcgtgct
      361 ggcggcgcac gatcctgggc ggcagatcgg cctggaccag ccggtacgtc catcgaagac
      421 gggcgtcgtc gagttgatct cggtctccga ggaagccgag ggaccaatgc tgtccgtaac
      481 ttcggcagag gcgctggcgg gacgcggact caggggcgac cgttacgcgc gcggcgcggg
      541 cacgttctcg gacccggacg gccgaggcta cgacctgacg ctggtggagg ccgaggcttt
      601 ggaagagttg gccgcaagcg gcgtggagat cgaaccggca gaggcccggc gcaacctcgt
      661 ggtgcggagc atcgcgctgg acgacctcat cgggaagcgc ttccgcgtcg gcggggtcga
      721 gtgtttcggc cagcggcgtt gcgagccgtg cgcgcacctg gagaagctca cgcgccccgg
      781 cgtgttgcgc gggctcgtgc accggggagg gctgcgcgcc gacgtgctct cggacggtga
      841 gattcggacc ggagaccgtg tagaggcttt gtcctaacgc ttctcgcccg aaagcgcgac
      901 gtaggcgcca agcccgatca ggacgccgcc cgagaggagg cgctgtccgc gccgcaggcg
      961 gacgctctcc cgcatccgtt ggccgagcgg cccggcgaag aaggcgacga cgaggtccgc
     1021 caccgtgttc aagaagacgc tgatgcagcc gagaagcagg atctggaacg cgactgagcc
     1081 ctgtgggttc acgaactgcg gcagaaacgc gagcaggaac agcgccgtct tcggattcag
     1141 cacctcggct acgatgccct ggtagaaagc gtctcgatgt ccgccaggct cgatactgat
     1201 ctccgcgggc gcctcgtcgc tgcccaaaag cgtgcggatg cccaggtaga tcaggtacgc
     1261 agcccccgct agcttcacga ccgtaaaagc cgtagccgac gccatcagta tggccgagag
     1321 ccccagcgcc gcccctaaga cgtggaccag cccgccgaga ctgttaccga acgtggacgc
     1381 gaacccttcc ccacgtccgc ccttcaggct gcgcgtcagc acgtagaaga tgccgggacc
     1441 gggcgtcacg gcgatgacga gcgccgccgt caggaacagg ccaaaggtcg tggcgtcggg
     1501 cataaagctc ctcctatggt agtcggctca tctctctcag cggcgcggct ggaaagctcg
     1561 ctcgccgcgt gccggctggg cggcaaaagc gtacaccgcc ctcagagaat cgcgtttgag
     1621 gttggggtag ctgtccagaa gctgctcctc gctccagccg ctggcgagca gatctagcag
     1681 gaaatccacg gagagacgtg ttccccttgc gaccggctgg ccggacaggc tccctgaatc
     1741 cgggtgtatt ttctctcgcc tgtccatcaa gctcttattc tactcctccg gccagcgcag
     1801 cttgggaatg acgcgctcgc cgtaggctct tatgaactct tcctggttgc ggccgcagtt
     1861 atggacatag acctcgtcga agcccaggtc cacgtagtgc tggatgtgct ccaggtgctc
     1921 gtccaggtcg gcgctcatca gcacgcggtt cttgaagttt tcgacgctga cctgcttcgc
     1981 catcgcggcg aagtcttccg ggttgcggat gtcgcccttg gggaagggca tcccgccgtt
     2041 gggccattcc ttgacggctt gctgctcggc ttcctcttga ttctcggccc aggagacgtg
     2101 cagttggatc attcgcggca tctcggacgg gtccttgtcc gcccggcgtg ctccctcctc
     2161 gaacttctcc atgagcatgt ttatcttctc gtccgccgcg ccgacggtga ttatgccgtc
     2221 gcagaactct ccggtgcggc ggctctggag cggtccggcg gtcgccacgt agatcggtgg
     2281 cggcgtatcg ggcatcgtgt atagcttcgc gctctcaagg gttatgtgct cgccgctgta
     2341 cttcacaacc ttgccggaaa atagcctgcg gatgacctct atagcttcca tgaggatgcg
     2401 aagccttgtc ggagcttcgg gccagtagtc tccgacgatg tgctcgttga gcgcctcgcc
     2461 cgcgccgaga ccaagccaga aacgtcccgg atacatcgct tcgagcgtag cggcggcctg
     2521 ggcgatgatc gcgggatggt agcggaaacc gggcggggtt acgccggtcc cgaagcggag
     2581 gctcgtcgtg gcgccgaggg cgccgagcca ggaccagacg aaggcgctct ggccctgatc
     2641 cggggtccag ggatggaagt ggtcggaggc catcaccgag gtgaagccat tctcttcggc
     2701 catcttgcac catttcaaga ggtccgtcgg atggaactgc tcgaacatcg ccgcgtagcc
     2761 tacggtaccg gtggtctgca tggcgtttcc tcctttagct tcggaacggt cattatagct
     2821 ttgggccagt tacggaacgg ttttctggct gacgctctag aagtgatccg gctcagagac
     2881 ccacggatcc ccaaccggga agtgcgcctc caggagatcc agggcttccg atgagctagc
     2941 cgcgacgagc ccgtaccgtg cgagttgccg tgctggaaga tacccggcgt agagttgcgc
     3001 gagctgcctg acgtcgagcg agacgcggtt ctccgcctcc gcctcaccag tcacctcgcc
     3061 gttcccgacg gtatattctc cggcgttctc cgggatccca tcgtccgaga cttcgaggac
     3121 gagcggctct cctacgagcg acttgaactt cagcgcgccc tccacgtcca cgagacgcag
     3181 catgaactct gtctctatct ccatcctgac gtgcgagctt cgaaggaaag ggtgaagcgg
     3241 ttcgccgcgg ggggtggagt gtttgacctg gaagaccagc gggtcctggg cggccatgaa
     3301 ggagacgagg gccttccgag cttccagggt atcggcgatg agctccgaaa cgttcagggt
     3361 ccggtaaggg tatttgtctc ggtcccagtc ggtcatgttg tagatggcgt aacccccgac
     3421 tttcccctcc cgctcgtata cggcaacctc ctgctccttg cgtccgagaa actgccgcca
     3481 cctcacctcg ttccggcgca cgcagaggtc gtgaccggaa gcctcggcat cgaataaagc
     3541 cgtcatgcga gaaaggtctt cttcccggta ggcccggacg tgcttctgct cggggctggt
     3601 cgggaggtcg gttggcttga aggtatagga gatggtctct ccggcaagct cccacccgtg
     3661 ggcccggtag aaggcgtggg agaagggcca gagcatggag aggcgcgtct cccgttcgcg
     3721 catgtcgcgc aggaccgccc gcatgaggtc tccggcgtag ccccggcgcc ggtaggccgg
     3781 atgcgtggta acggccgcga ccccgcccat tgggaccgtc ttcccgccca ggaacacttc
     3841 cagcggcaga accgtcgccg tggcgcgggg ctcgccgtcc tcctcgatga tgtggacttg
     3901 ttctggatcc aggcgagcgt tctcggggtc gtagtatctc tcccagtaac ccgaaccgcc
     3961 ggggtccccg aacgcggtct tcgccagccg cgccagcgct tcccggtcct ccacctgata
     4021 cccgcgtacc ttcaattctc ctccgagaaa gtcgcctgcg cctggattct atccgaagcg
     4081 gcgacgctcg ctaggactta ctgtcccttc ccttcttgcg tctcccggac ggctccgctt
     4141 tgccctctat ggagtcggcg acccagcggc accactgagc gtactctgtc tcgtagccga
     4201 tgccgcgccg gagcgaggcg tagccggcga accagggaga gtccggacgg ttcacctcct
     4261 cgccgtgctc gctctccatc cagtcccgcg tcttctcgta gcgcgcgagc tgctcctcgt
     4321 gccggagttc ctcctcccgg aagagccgcg ccgctttctc cgggtcggcg agccagacgc
     4381 agtatgcctt gagcaccagt tcgtcgcggg tgggcctggg tgacggcggt tccgtaaccc
     4441 attctttgag ggtttcgagt cctgcggccg ttatttcgta gaccttcttg tccgggcggt
     4501 cctgctgggc gaccacgctg tgcgtcacgt atccgtcccc ttcgagccgg gcgagttccg
     4561 ggtagatctg actgtgcctc gcactccaga agttccccac ccttcccttc atccgctgcg
     4621 tcaggtcgta cccactgaga gcctcctgtg ccaataaccc caggatcgca tacccaagcg
     4681 tcgtcatatc cctccataaa tttttgctat gtcactattg acatatgttt tctcagtatg
     4741 tagagtacca tatgtcataa tttacataaa gaattggtga aaggagcggg gcatgtgtgg
     4801 caggtcatac gcggtacgcg gggcgggttt cgcgtcgggt cggcggaggc tcttcgggct
     4861 ggacgcggag aagatgagtc cagaggttag ggtagctgtg gcgctctcgg tgctggggcc
     4921 ggtggcgctc tcggggatct ttctggtggt tttcgtgcct tctctgtggt ggatcttcac
     4981 gacgtacttc tgggtctcct ttccggcgct cgggatgctg gggagcggca tcgccgggtt
     5041 gagtaaggcc cgtccggctc gggtctcgga ggaggaccgg gaaagggagc tgttgacgtc
     5101 gctcgcgcgg cacggtgaag tctcggcggc agcggtggcg gcggagacct ccatgacggt
     5161 gtccgaggcg gatcggaaac tcagggagct ggccgagggc ggacacctgg aggtccgggt
     5221 gcggggcggc gccatcttct acgcgctgtg ggaggcgaac ccggtggagt ccggaagcga
     5281 gatcgagggt gtgcggtgag cgccggagcg agcggaatga cggtctccag ggcgagcgag
     5341 gagaagacgc ggcggaggtt gctcatcttt gcgggcgcgg cgacggtctt cagcgtcctg
     5401 caccacacgg accacgtgat ccggggcaac cattccggct ggccgttcgt ggcggagatc
     5461 acgcctttca cgttcagcct gctcatctac gctctcatac tgccgggaat ctacctgacg
     5521 gcgagggcac acaagatagc cggatatcat ctgttcgtcg cgatcgtggg gctggcactc
     5581 ctcggcttcg tccacttcgc gccgctggga gactacgagg ccccgatcag cgacatctac
     5641 atggcctacg acagtccagt cgtcggactc gccgctcact tcgtgttggc gggactgatc
     5701 gcttgcgtgg cattattggc ggtgacagct ctcggagcgt tgcgatccag ggtgagaata
     5761 cttgattccg agaggggatg actgatgaag acacggaaag tcattggcac caagcagccg
     5821 ccgtttagag cgcaggtcgc gttcgcgcgg gcggcgacgg gactcggctg gatgagcgtg
     5881 ggcgccctgg cggtgggggc aactgcggtc ggggcggtag ctataggggc tctggcgatc
     5941 cggacgctcg ccgtgaagcg cgggaagatt gagcgcctct ccatagaaga gttggaggtc
     6001 ggccggctgc gggtgcggga gttgatcgtc gagagcgaac agggcgagga ggactagcag
     6061 atggcccgga tcctgaacgg caggtacacg gcgcgcacgg atgaaccgtt cgtcgtgttc
     6121 atcatcggga tgaggattaa caacctcttc gccgtcaaca agtggctccc aacggcccgc
     6181 gcaatgggac ctatgctaaa ggaactctac cagcatccag agaagggctt tctcggcgcg
     6241 gagttcttca tgtactggcg cggtccggcg ctcgttcagt actggcgctc cttcgaggac
     6301 ctggagcgct tcgcccgcaa ccccgacgat cctcacatgc ccgcctggca acgcttcaac
     6361 cgcagcgtcg ggaaggacgg cagcgtcggc atcttccacg agacgtacat agtggagcgg
     6421 agtaactacg aggccatcta ctcgaacatg cccgtcttcg ggctcgccaa ggccaccgag
     6481 cacgtccggg cggtcggagg gcgggagacg gcgcgacggc gcctgatgcg cggagacaac
     6541 gaacctgcgg tgccgtctcc agagtagagg tggtagcttc tagtgtcccg ccgcctgcca
     6601 ctctctgatg gcgtctagcg ggtagatcag catgatgatg ttcaggagca ggccgtcccg
     6661 gatccagaac gccagcgtca cctccgtcgc caccaatacc aggagcgtgc gccggaaccc
     6721 caggcgacgg gccagcgcga acccgagccc gcacaggacg atgtcggaga gcgagttgac
     6781 tatcgtgtcc ccgtggtagc cgagcgcggc ggtcccttca cggtagcgcc ggatgacata
     6841 ctccgagttc tcgactacct cccacacggc ctcgatggag accgcgagcc agagcctcca
     6901 gatcaccggc aatcgtggga tgatcagggc cagcaacccg tagaatatga agccgtggag
     6961 cacgtgcgta aagctgtacg ggtcgagcag gtgctgcgag ttatccgagc tccaggcgtc
     7021 gcccgaccaa agaaggagat agtcgcagga gcacaaccac aggcgtccct ggctacggag
     7081 ttgatacgcg gcacccgcga gtaccgcgat gatggcgagc cacggcaacg tctttcgcat
     7141 catgggcggc agcgtacgtg atagacgtcc gtgctgccag catccgggac cgccggagta
     7201 gagttactgt tcggggagtt cgtcctcgtc gccgtgtcgc tcggccttgc gcgcctgctc
     7261 cttgaccttg ttgccgacga cgcgcgtcca gatcacggtc ccgatgacgc tggcgattac
     7321 cactcccgtc gcgaggacca ccatcgttcc tgttccttga gggctcttta tgaccagacc
     7381 ggagaggtac aggacgagcc ccacggtgag gataaagaag ccggcgcgct tctcggcgag
     7441 gagctgggcg tgagtagcga tagcgtcgtt ctcgccaacc gcgctcttct ccgccccgat
     7501 ctcggcccgt atctccgcta tcgactccgc gttgtgcgcg tgcgagagaa ctcccgcccc
     7561 cacgagatcc atcgccacgc cactgacaat gagcgcctcc gtcagaagac tcatgaatgc
     7621 accttccgtt ccaaaaaacc ggctaacggt agcacaggaa tccactacac ttcatcgctt
     7681 ctatcctgac accatgatat ccatgctcct ggcggcgcct acaacaaacc tcttcactac
     7741 ccctatcgta ccagcgaaga tcgcgctgct gtacgccaaa gacctaatcc ctttccaggg
     7801 cagagtggaa gagacaaaag actcttccta tgacacaaaa atctaataca gagacaaggt
     7861 ggtctatttt cgcatctctt tcttgtatca ttggtattaa taaaggttgt tgggtcgggt
     7921 atgtaaatga gccacagatg tacatgtttg ttgctgatct cagggatgtg gctattcgcg
     7981 ctcgtcggcg gatgtgtacc gtctgcagat acgaactcgc cgacaaccgg gagcaacgga
     8041 aatacagcca ggtcagccga gaggaactct gcatccagcc cattggaaga taaaccgtgg
     8101 cgactcgaaa ctatcgtccg aaacggcgag ccggagcagg tcacggaaac cctccggcca
     8161 gccatcgcct acttcaagtc gggtaaggtt ggtggacgag ccgcatgcaa ctactacggc
     8221 ggtccctacg aggtagacgg aaagaaactg gccatcggcg cgttgatggg cacgtcggtg
     8281 ggctgtggcg gccataaagg cgaagaagaa aaagctcttc ggaggggaat gcaatccgct
     8341 gagagctaca ccgtcgaagg cgaccggcta accttagcct tccccgacgg taagctcatc
     8401 ttccaagcct tgccggatgt tcgtggcgac gagcaagccg tctacgcagc agtcctggaa
     8461 gagtgggatg gtggacctgg accaatcgtc atcgtcaaca aaaacagaat cggtcccgct
     8521 ggcagaaacc tggacgatgc cgtagacagg atacggagag agaatgctgc gaggcccggc
     8581 tacaaaccga tactggacgc cgaaaccctg tccgacttcc gggataagaa cacagggacg
     8641 ctggacatga gtagagtcgt agacctcgaa gtcccgacgg tgttcgtcag cgaagcggag
     8701 atcgaggccc tattcacaga tggagaagag gctggctgga gtaggttcaa gaaaacctat
     8761 ccgggtgcgc gcgagatcat ctctctgtcc cgagtaggct tcaatgggaa cggagaccag
     8821 tccctcgttt acatcggcgt tcgcgccaag gactacgaag agggacacta cgatctcatg
     8881 aactaccata gcgggctctg ggtgagcacg agttgatctc gttccaaagg ccttcgcaaa
     8941 cttcgtgcct gttcctctag cggcggatgc tgggaccgac gcgcttcatg gcggtgcgga
     9001 agtcgtaggt ggtgacggaa agagcggaga gttggtgcga ggctatgcgc ttctcgaagt
     9061 cgcgcaggtt tatcgagcgg cgcatgcaaa ccagggcggc ctcgcggcac agggcttcga
     9121 ggtccgcgcc ggagtagctg ccggttatct gggcgatctg cttcaggtcc aggtccgggt
     9181 cggtcggtat gttcttcgtg tggatcttga gtatgtgaag ccgcgcctcg gtgtcgggca
     9241 atcctatctc tatctcgtgg tcgaagcggc cgggccgcct gagcgccgaa tccagcgcgc
     9301 tcggccggtt ggtcgtggct atgacgcaca cccttccgag gtcgttcatg ccgtccatca
     9361 gggagagtag ctgggagacg agcgtcgctt caaaagactc gctcatcatg tcgcgggagg
     9421 aggcgaagga gtctatctcg tcgaagagga tgatcgcggg agacttcgtc cgcgcctcgc
     9481 tgaagatgga acgcagcttc gcctcgctct ggccccagaa cttgttgagg atctccggcc
     9541 ccgacaccgc gatgaagtgc gccccgctct cgtgggccac ggcgcgcgcc aggagcgtct
     9601 ttccggtccc gggcgggccg tagaacagga tgcccttgtg cggacggatg ccgagcttct
     9661 tgaagatctc ggggtgcgtg atcggcagct ccacagactc gcgcacgagc gctatcgtct
     9721 cgcgcatccc gccgacgtcc tcgtagcgcg tctgcggcac gttgcggctg cccggcgcgg
     9781 ccccgctcgc cgccgccacg ttcaccccgc cgctccgcac gctctgcgcc agcctctcca
     9841 gcagcgtctg cttctcctcg gcggacgaag cctcctcgat accttcgtgg gtcttggaga
     9901 ccttccagtt cgtcagcggc ccgtcctcgc ggtagcgcca gccgtcttcc ctgctcgggt
     9961 cgatctgctc cggcaggcgg gcgaccagct cggcggtcgt gtcgaggtcc aggatggggc
    10021 agcgcacgca cggcccgagg tccgagagga cgcggtcggt gatggcctcc ttgtccacct
    10081 tgaagacgcc gtcctcgccc atctccccga aaccgtacca ggagctcttc gtgagatggt
    10141 cgttgtcgta gacgtggatc tgcttgcatg ggaagagctg cttgccctcc agggactttg
    10201 gcgtgcacca ctccctcgac gacgccccgg agcgctgctt gcgcaggaaa caccgcagac
    10261 cgtcgaagag ctcctcgccc gagagcgcca cgccgtccgc cgtgtaatcg actcgcttct
    10321 cgccacgcaa gaggttcgag aggtcatcca gcggacgcca gtcggccggg agatccaacc
    10381 cgagattcac ccgcgccgtc ccgttagccc cttccacccg ataggaagga tgccgggatg
    10441 cgatggacgc cgcgcgtccg agggggccgt ccgggatcag tgggaagctc acgcgaagtt
    10501 tcataacccg cagattttaa cctacggtac gggaaaccgt cggacggccg gcaacatgcg
    10561 tagacaggct ggcttcgtcc ggccggtaaa ccctgcaagt cctgtcccag gctataataa
    10621 cgaaacttac aaatctcacg tcagcgatct cgctgcggct gtgatgattg cttgcgtggc
    10681 cgggtgttcc aggttaggtt gctggcgaag gtcttactag aatgggagta cgatgagcga
    10741 gaggcgagag cagcccgccg gtctcgaccg tccgcggtgg tcttcctgtg ttgagcagga
    10801 tgagcaagag caccccatgc ctctccggac tgcggttcat cttcgccaga gccgccggtg
    10861 ggaagaagcc cctctcgcgg agccaggctg agatccaccg ctcccttcta ctggtcctga
    10921 tcccgctcct cctggcaata gcctgctccg aaaccaccca aaactccgat acatccgcac
    10981 gtacggcccc acagacgaag tcaacctccg gctccggcga tgccgcggct ggcgataccg
    11041 tatccgggtc ttcgccaagc tggactagag atcccgcccg gaccagcatg gcctcttcca
    11101 tgacgaagga gacggccacg gtctacggct acacctacgg tcaggcttcc ggcaaccgag
    11161 tggtcgaagg caggggccgc ctgcccgaag ccaagccaat cgacgtgtcc ctcggcggca
    11221 ctcccacttg ggtagtcggg gtgccgctgg aggacgacac agcctgggtg gtcgcttacg
    11281 gcgatggccg ggtggacaca ttccggctcg acggcgagag tggcaaagtg gagccctggc
    11341 tgacctcacc ggacaaactc ccgccaggcg ctccgccggc tgtcgcggtg cagggggagc
    11401 ggctggatct cataaccgcg cctggtaaac gaagctcatc cctgacccat cccatccgcg
    11461 gggaggccgg gcttctcggt atcacggcgg gcgggaagct gtttaccgaa cccggagaag
    11521 gtccgcggct ctccgcgctc ccagacgccc gcatcgtcca gagttcgagc ggcgcggcgg
    11581 cggtgctcgc ggcccccacc gcgcgttacg accacggtat cctcggagac aggctggaag
    11641 cgggcgagat cggcgtgctc aagcccggag gaggaggcta caggctcacc acggaagtac
    11701 agccggaatc cgggggcgtc ttcgaggcgc tcgcgccgct ctggttccgg cccggcgccg
    11761 gagaagacga gttgctggcc gttacggaga gcgcagagcg gcggggatcc aggatctccg
    11821 tctactcccc ggatggcggt ctcgtcgccg ccggcccgtt cataaacgct ccgcagcagt
    11881 ggcgtcacct gatcgcggcc ggaccgttcg ggccggacgg gcagatagag atagcagcca
    11941 tgcgcactcc gcacatcggg ggtacgacgg agttctaccg cctggaccgt aagagcggag
    12001 agctagagct ggcggccacc ggccccggtt acccgtctca caccatctac agccgcgacc
    12061 ttgacgcggc ccgtgccgga gacctcgatg gcgacgggtc ctgggagttg ctcgtcccga
    12121 acaccgccta caacggtctc gtcgctctgc gccatacgaa gaacggcgtc gagaaagtct
    12181 ggagtctacc ggcgaccaac tcgctcggca ccaacatcgc cgccgtcacg gacagcggag
    12241 gacgcatcgc cgtcgccgtc ggtacgctgg atggaaacct cagaatctgg cgctgagagt
    12301 ccgggaggag atcaaagggt aagataccgg gcgtcgagaa agagagaagg gggttgcaag
    12361 tggcggttga tttcggggtg ttcgtgccgc agggttggag gatggacctc gtcgggatag
    12421 agggcccctt cgagcaatac gagacgatga cgagggtagc gaaggccgcc gacgagagcg
    12481 gcgcctacga ctccatctgg gtctacgacc acttccacac cgttcccgaa ccaaccatga
    12541 acaccgtctt cgaggcctgg accataagcg ccgggctcgc ccgcgacacc aagaacgtca
    12601 agatcgggca gatggttacc tgcaacggct accgtaaccc ggcgctgctc gccaagatcg
    12661 cctcgacggt ggacgtgatg agcaacggac ggctactgtg cggactcggc gcgggctggt
    12721 acgagcatga gtggcgagcc tacggctacg ggttcccgga agtcccggtc aggatgcgga
    12781 tgttcaagga ggcgtgcgag atcgtccacc gcatgttcac cgaggaccat ccaaccttcg
    12841 acggcgagtt ctacactatt gatcagccca tcaacgagcc caagggcgta agcgatccgc
    12901 acccgccgat ctggatcggt ggcggcggcg agaaggtcac cctgaagctg gtcgccaagt
    12961 ggggcgacgc gaccaacgtc aacggcgaca tcgagaccct caagcacaag ttcgaggtgc
    13021 tgaagggcca ctgcgagaac gagggccggg actacgacga gatcacacgc tcgactagcg
    13081 ttaacgtcca cctcctcgaa gacggccagg accccgagga ggccacaagg gaagcccgtg
    13141 gcgagatgag ctatgaggag tacagcgagg gtcctcacgc cttcatggtc ggcacaccgg
    13201 agcagatctc cgagcgcctg caaccgatgg ttgacgcggg cgtcgagtac ttcatcacct
    13261 acctgccagg cgtcgcccac gacctctcgc cggtcgagcg tttcgccaac gaggtcatcc
    13321 cgaacttccg ttaggcaagt ctcctgctag gcaggtttct ccacccactc gggttcgggt
    13381 gtgatctggg cttcgggatg cgggccgggg ccgtcgaggc cggttgaggt cacggtcatc
    13441 aggacccgga ccttcatgtc gtggtcgcag gcggcctggc acgagaggcg gacctgtccg
    13501 aggagatccc gcttctccag cacgtccagc tcggccttcg tcatcttctc gggctcgccc
    13561 tccaggtact cgatccggca ggtggtgcat ttggcgtagg agccgcagcg gtggaggatg
    13621 tccaccccgg cgtcctcctc tatcgcgagg accagccgct tgccctcctc cacatcgaac
    13681 gctcccacac cttcgacctc gattctcggc attcgctcct ccttatgttg gccggtggtt
    13741 acgaaaacgg tcattatcca ccggcccata gtctggcgcg agggttcacc cgccggcaga
    13801 tgctctcgta agatagtatt ccggcagaga attacatctt cccgctacat cttctggagg
    13861 actcatggtt gggcgagcgg cggtcatcag atacgtgaag ctacatgccc gcccggctac
    13921 ggtcgcggcg tttgactctg ttggagatcc tctctgagtt ccgtggtgcg ggacgtgtat
    13981 aaaagtgctg gcttatgttc taaactgcct ccgttatgag aggggagatt agcttggatt
    14041 acaggccgcc ttcggtacga gtagagaagc cctggggcag gttcgagcag tacactcaca
    14101 acctgccctg caccgtcaag atcatcaccg tcgcccccgg cggcacgctc tcgcgccagt
    14161 accatcatca gcgcgacgag ctttgggtga tgttggaccc cggcgcgcgc gtggagctgg
    14221 acgataaggt cctccatccc gacccggagg agaagctctt catcccccgc gagacggtgc
    14281 atcgtctctc ctcgacggga gataagccgg tgaggatact tgagatctcc tttggagagt
    14341 tcgacgagga ggacatcgtg cgcctcgaag acgtctacgg ccgcgtagaa ccgtgagata
    14401 ttgcctctcc aggagcggct agaaggtgag attcttcggg agaggagaag aaccggatca
    14461 tcgaggagcc gatcccggca agggtcccga ggatactcca tccggcacag aacgtcggcg
    14521 ggaccggaga cggcgtatgc cgtggctgct cgttctgggc ggcgtcctgc tactgttcct
    14581 gctgctggga gcggtcatcc ttctctggcg ctccttcagc gacagcctcg ccggcaccag
    14641 cgtcgcccgc gactctatag actccggtcc gaaacccggc atcaagctga cgaacggggc
    14701 gggacagatc cggatcgagg gcgtcgagga cctcggttcc gtcgagtacg aggtaacccg
    14761 gcacgccctg ggccctgacc cggcaaccgc caaggagcgc gcctcgaagg tgactgtaaa
    14821 cgtcgcgcgc gacggtgggg actttgtcat cgagacggac ggtggccgga acaccggcgc
    14881 ggactacgcg atgcgggttc cggccgggag ctcagtggac atagagtccg gggctggcga
    14941 tgtcgaagtc tcgaagatct ccggcgacgt gtcggtggac gccggggctg gcgatgtatc
    15001 cgtgcgggaa gtgaagggct cggtaaaggt caaagcgcaa gcgggcgacg tcgaggtctc
    15061 gaagatgcag acggataccg gaggagcgga catcgaggtc gcctccggcg atgtgaccct
    15121 tcaggatctg gtcgccggca ccttggagac cagtgtcgag gccggcgatg tgaccctctc
    15181 cggccgcttc tccggcggcg ggcgcgtctc cgtgaagacg ggcgatatca tcgcgcagct
    15241 accctcggag gacacccgcg acctgacgct ccagacccag gtcggccagg tcgtgcgcga
    15301 ggacgccgga ggacccggca agaagccgga gaagagttag tgttccgccg cagcggccag
    15361 cagagtatgg gctactctcc ggagagcttc cgggaggccc tgaaggaagt gtggttcgcg
    15421 ccgggacggt tcttcaagcg gctcgacccg gagggcgggc caatccgacc gaccatcttc
    15481 gcctccctcg tgctgtacct gaacctgctg ctggatgcgg ttctacaggc gttctggctc
    15541 ggcgagtaca acctctcact cgtctacgcg ccgttcgttg ggctgctggt cgcgctcgtg
    15601 ctggcgccgc tcctgatctc cggtctcacc gcgctgacgc tcgttatcct tgatggagcc
    15661 ccgtcgcggc ggaacttcgg tcccgtatac cgggcttttg gctacgccag cgggataggg
    15721 atcgttgtct ggatccccta cggaccgcta ctcgccatcc cctacggcgc ggccgtcgcc
    15781 accatcgccg taaaggagac cctcggcctc ggatggaggc gcgccgccgc cgcggccctg
    15841 ataccactcg gagcagtatt ccttatcgta ctgttactcg tgggcccaac cgaagcctac
    15901 gggttcttga tcaaccccgc cggcagctag caacccgcta ggacaaagcc tccagcgcga
    15961 gcgccgcaag cagcgccgat ccgcgcggca gtacgtcctc gtcgaaggcg aactgtggag
    16021 tatgaaggcc ggatgcgtcc tccccgatcc cgagccagat gaaagcgccg gggatcttct
    16081 ccaggaagaa cgcgaagtcc tcgccgccca tcgaggggtt cggaagctcc agcacggact
    16141 ccgcgccgaa caggttctcc gccaccccga gcgcccggct cgccgcccct gggtcgttca
    16201 tggtaacggg ataggagaaa cggtagtcca gctcgacatc gccgcgcatc ccgcccgcga
    16261 ccccgcgagc cagttcctcc atacgctccg gtatcctccg gcgcagctcc gcgttcaggg
    16321 tgcggaccgt tcccccgatg cgggcagtct ccgggatgat gttgaacgcc gtgccagccc
    16381 caatctctcc gaccgtcaac accgcgggct cgaccgggtc cacctcccgc gagactaccg
    16441 tctggagcgc ggtgacgacc tgcgcggcga tcacgacggc gtccgccgtc aggtgcggca
    16501 tcgcgccgtg cccgccggag ccctttatcc gcatctcgaa cccgtcggcg gcggccatga
    16561 tcggcccggc tttcgtcgcc accatgccga ccggcagacc cggccacagg tgcagagcga
    16621 atacggaatc cacgccgtcc gctgccccct cctcgaccat gaccttaccc ccgcccccgc
    16681 cttcctcggc gggctggaag aaaaacttca cggtcccgtt caacctgtca cgcatccccg
    16741 agagcgcgtg cgccgctccg accagcatgc tcgtgtgtcc atcatggccg caagcgtgca
    16801 tctttccgtc ggtctccgag gcgaacggca gcccggtctc ctcgtggatc gggagcgcgt
    16861 ccatgtccgc ccggagcccg atggtcggcc catcaccccc gccgcgaagc gtcgccacga
    16921 tcccgttctc agcgatgccg gtctgtatct ccagcggcag accttcgagc gcggccagga
    16981 ccttctccgc cgtcttgacc gtatcgaagc cgagctccgg ctcgcggtgg atgtcgcggc
    17041 gcacggtgac gatcttctct ccgaaatcct tctctatctc cctctgaagt ccatcaaacc
    17101 tctgggtaac gtccatcggg cctcctctca gcctgccatg gccacaatgc tcaacgcact
    17161 agcggcgtag gagaaactta tcatagagag ctgccgactc tcctggatca ccccgatccc
    17221 ggagaccata ccgtcggctc ttgcggcagg cgatcgggat cgaagcgcgt gagcatatcg
    17281 ttgattgttg gtcgctctcc gggtccggcc agaccgagcg agcgtgccat ccagccgaga
    17341 acatcctccg gactctcgcc ctcggcggcc ctatccgcga gcgttaccgc gccgtgacgc
    17401 ttggcgaggc gctgtccgtc gggtccgagc acgagcgaaa catggacgtg acggggcacg
    17461 ggcaggccca gcaatcgcgc cagcaagacc tgccgcggtg tagagtctaa gaggtccgcg
    17521 ccccgcacca cctctcctat cccctgggca gcatcgtcca ccaccacggc tagctggtag
    17581 gcgggcgcgc cgtcgttccg ccgaaccacg aagtcgtcca cctcatcctc ccgaaaaccc
    17641 agcagccggt cctcgaagga gacgcgagcc tcattcgacc gtaccctcag cgccggtggc
    17701 cgtccggcgg cctcacgttt ggcgcgctgc gcggccgtca gctcgcggca ggtcccagga
    17761 tatcgatcgc cagcggagat gccgtgcggc gcggaggcgg aggcccggat ctccgcccgc
    17821 gtgcagtagc aggggtagag caagccatca gcttcgagcc gggcgatagc atccgtataa
    17881 agctccatcc tctccgattg acgcaccacg ggctcatccc aatccagtcc cagcgcccga
    17941 aggtctgaaa gctgcgtctc ttccatccca ggccgcaccc gcgaggggtc taggtcctcc
    18001 acccgcacca ggaatcgcgc tcccgacgac cgcgcgaaca gccacgccag caacgccgtc
    18061 cgcaggttac cgacgtgcag cggacccgtc gggctcggag cgaagcgtcc atcggcattg
    18121 tcaacgtttc cgacccttgt ctgctccgtt ccatacacag caaaagggag tctacaatct
    18181 tctcaagatc aaaacgctcc cccggcaaat ggtcagagaa gcgtaattag ccgtaccaca
    18241 atagcgttcg ctgctcgctt gcgtccgcac atgatacgat cctccgcctc atgccgcact
    18301 ctctcatcca tcatgcgtcc tcccgactcg ccgaccgccc gtagaaccgg tcgaactccg
    18361 cgtccaaccg tccccagagg tcgtcctcgg agatccccaa cccaacagcg tcccttacca
    18421 gctcgccgag cctctcgttt agtgcctcaa tcctctgctc ccgcgccgtc tcccccacgt
    18481 cctgggcgac taccgtaccg acgcccggct tgctctctat gagccccatc cgctgtaact
    18541 cgttgtatgc cttgaccaca gtgttcggcg cgaccgtcaa ctcttcggcc aacgcccgca
    18601 ccgtaggcaa tcgctcccca gcctgtagcc caccgacctc cacagcccgg cggacctggc
    18661 ccacgatctg gacgtagatc ggaaccccac tccgcacgtc aacgtccagc catagcgcca
    18721 ccaacatcac ccctctagtt ctattgtgtt attattatga ttcaatagta tggtgatgtt
    18781 gagtctatgt caagacctgc gagggaaaca caagagatga cttcagggtc aggggatcca
    18841 gactcgaccg ggagattcct gggggaggga gctgaggacg accttcttga tgcccattcg
    18901 ccagatctcc ctggttatga tcgaggcgtc ggacatgctg cggccgggct ccacgcctgt
    18961 tatgaggatc gcgggttgag cttcataggg gatggaaccg agcgagcctt tcgccagtgc
    19021 tcgcgcgaag gcacgcgctg tgatgctctg tccgagactg atgccggtga ggtcggagaa
    19081 aatctgcggg cggtggacgt gctcctcgtc caccgggcta cccagcgccc gtatgttgcc
    19141 gagtgcgact acgagggtcg ttacatctgg cagtgccggc tcgtggtcgg ccgttccctt
    19201 gagaagccgg tgacgggcgc cgtcggcttc gaccaggacc acatctgcca gatccgccag
    19261 cgttccgaac aggtttggtt cgatcccacc tacccgcccc ttcgagatga gcgagcttcc
    19321 ggccaccacg gcgttcgcgt ccgtgagagc ctctctaacc cgcgcgcgaa gctcctccgg
    19381 gtcctccgag gttacgagag gcccgatctg ctcggcctcc gagaccatca tcttggtcgt
    19441 cggggcgacc agcacccgca taccgtctct ggtgagaccg gccgagatct cccgtatggc
    19501 gctggtcttc cccccggcgc cggcaaaagc aaccacgtcc ccggaagtaa tccccaacgc
    19561 caggtaaaga ttcacaccct ctcctctcct gtccctacgg ccacgcttgt gcgcgtactc
    19621 ccatgttaca tcatagcgcg agcttacggg ctttatgata tgttggatac tataaggcta
    19681 gcccatgaaa atgccgccgg ctaatttggg gttatgcggg aagcggcgag ggtaaagaga
    19741 cgttaatata atgtttagga cattgaaaga aggagtgttg tggcgcaggg acgagaacgg
    19801 gtagcagagt tcggtcggga gttgagggag gggccggagc cttcggaccg gagcatcaag
    19861 gagatagtgg atgctctcag gccgcagctc caggagcttg cgagcaagca ggtcgagttg
    19921 gcaaagaccg agctcgcgcc ggtcggcagg aaggcgggca tcgccacggg tctgctggtc
    19981 gtgggggccg tctttctaca tcttttcctg gttttcatct cggtcaccgg aatctacctg
    20041 ctaaacgagg tcgcgggcct tagtctgtgg ctctcggcgc tcatcatctc tggtatattg
    20101 ctcgtcatcg gcgggatact cgccggggcc ggagccggac agttgagggg gctggatccc
    20161 aaaccccacc gcacgatcag gactatgcag cagaatatcg agtggctgaa ggggcagttc
    20221 aggtcatgag cgagatacca gagcgtatag agagggagat gttcgagatc cggagccgca
    20281 tggccccgga tgtgcgcgac ctcaagcagc acaccaaccc gcaggtcgtc ggcaagcagg
    20341 tctcagcgaa gctcaaggag cgtatcaagg gcgctgttac cgggtttgca cggagcctct
    20401 ccaactccgc caagcgccag gtaaagatgg cgagggaggc gggccgcaag cggaacccgg
    20461 cccctttcac cgatgcggtc aagagtgacc cccgcccgct cgtcctgctc gccttcgcgt
    20521 tgacggtggt gctaatgacc gcccgcaaga tctcaaacgt ccgtaactag ccgaatagtt
    20581 ttccagactc taccgggctc cggcccgtgt ccgggttgcc ggaaatacag gctcgcgccg
    20641 tgacccacgt ccacttccaa cccatcggcg gcgccgccgg cgatatgatg ctcgccagcc
    20701 tcatccatgc cggagcctct ccagacctga tctccggcgg cctcgacaaa cttggagtcc
    20761 ctttcgagct gcatgtcgag cgtgttgagg tgaacagcat ggcggcattg cacctgaccg
    20821 taacgcatcc cgaagaacac acccaccgcg ccttccgcga catccgtgcc ttgatagaga
    20881 gcgcggatct gccgcagcgg gtggcggaac gcacaagcac agccttcaga catctcgcga
    20941 tcgccgaggg cgcggtccac ggcaaggatc ccgaagaggt gaccttccac gaagtgggcg
    21001 cgattgactc cataatagac atcgtcggga gttgcgtggc gttagagctt ctcggcgccg
    21061 ggaccgtgag ctgcggcccg ctcccgatgg gtggcggttt agtagatgcc gctcacggca
    21121 agctccccat accaggtccg gcgaccctag aggtcctcaa ggggtcccgc gtgcggtgga
    21181 cggaggagag gcgtgagatg accacgccga cgggcgccgc gctcatgcgc gccctgacag
    21241 gcgggaaatt cacagatgcc gcgccgccga tgacgcttgg cagcatcgga tacggagccg
    21301 ggcgggcacg gttcgagacc gtcccgaacc tcctgagagc tgtcgtcggg gagatcgaag
    21361 gttccgggag cggcgtggag gtgctggagg cgaacgtgga cgacgctcca ggtgagcttc
    21421 tcggagcatc ggtggagagc ctgctccatg caggcgctct cgacgcatgg ctggagccta
    21481 tcttgatgaa gaagggccgt ggagcgtaca aggtctgcgc gctcgccagg accgaagacc
    21541 gggaccggct ggcgcggacc atgatgctgg agaccggcac gctcggggtg cgtcatcacg
    21601 cggtaggaag aaccgtcgcc gagcggcgga tcgtacaggt ggatctcccc tatggcccct
    21661 gccgcgtcaa ggtggggagc ctggatggtg aggacttcgc cgtggctccc gagtacgagg
    21721 acgcggttcg tctggcgagc gcatcgggac tctcactgcc acaggtttac tccgatgcgc
    21781 gacggatctt cgaagcttcc gggggaaatc ggataaagaa aaaggaccgg ggctgacccg
    21841 gtcctttcgg ctttcgactc tgcgaatgcc tacagaggag agacgttctc cgcctgcatt
    21901 cccttgcgtc cctgcgtcgg ctcgtaggac accttggtgc cttcttcgag agacttgaac
    21961 ccgttgccgg ctatgccggt gtagtggacg aagagatcct cgccaccgtc atcgggagag
    22021 atgaagccgt agcccttctc gtcgttgaac cacttaactg ttccctgggc catgtacttg
    22081 agcccacctt tcttgttgcg ggccagtcca ataaaaatgc cgcctccgag tttccaggcg
    22141 gcgtttcacc agcactgacc atcttccatc tacagtccgg tagtctagta caaaactccg
    22201 ctgtctgctg caaatcaagg ccatatatcc atctttatca acgatacccc gctcataccc
    22261 tagaatagcc tcatgaaaga gacagatact cccaagcatc ccggcgcttc gtacagcatc
    22321 accggcggat acgcagctct gaaagacggt gccgccatcg tggaccaccc ggaccgcgcg
    22381 ctgttgcgtc tctccggcaa ggacccccgc ggcatgctca acgctatctt gagcaacgac
    22441 gtgccggcgg atgagagccg tggcgtctac gctatgctcc tgaaccccaa aggacgcatc
    22501 ctctccggcc tgcgagtcct caagagcaac gaggagatcc tgatcgaggc ggaggccgga
    22561 ggcgcggcgt ccgcgaagga gacgctcggc cgctacgcgc cgttctctcg cgtcaaggtc
    22621 gaagacctct cggactcgtg gagcgtgatg ggcatctatg gtcatggagc agagactctg
    22681 ctggattgtc ccgcccttgc cgagcacgag acggcggaga tagaggtaga tggcgcgaca
    22741 ctgctcacgg ccggggtgct cacgccggcg cccggctacg acctgatcgg tccgtcagag
    22801 gtcgtagccg ccgccaggga ccggctggtc gcgaggggcg ctactccagc ggaccacagc
    22861 gcgtacgaga cggtccggat cgaaaccggt gtcccccgct acggagcgga catcaccccc
    22921 gataacttcc ccggcgagac gggtctgctc gaacgagccg tcaacttcca caaaggatgt
    22981 tacccgggtc aggagacggt cgcccggatg cactaccgcg gccatccgaa caagaccctg
    23041 taccggctcg ctatcgaagg ccatccacct gagctcggcg agaacatcat ccaggctggc
    23101 aaggtggtcg gcttcattac gagcgtcgcg cccctgccgg tagaggaccg gatcctggcg
    23161 ctcggctatc tctcacgcac cgcggatatc gaagaaccgc tgcagacccg agaggcaggg
    23221 atctctcctc tcggtcccgt ggggtcttga tgcgggactg agagcctctc cggcccggat
    23281 acagccgggc caccgttcta ctctccggtc tttagctgct cggcgattag gttcgcgtag
    23341 aactgcccgc cctcgggacg caggtgcagt ccatcgtccc agaaaaattc tggatggttc
    23401 acgctggcgc cataccagtc gactagctcg gctttcggat acttcttgac tccctcggcg
    23461 agtacggcgt tgtttggctg ttcccacgag cgagggacct tcaggttcac gattatgacc
    23521 cggttcgcgc tctggagcgc ctgcatcatc tggtcgaact cggcggagga gatcggtccg
    23581 ttattgccga tatgcacgat cacggtgtcg ccgatctgtc cggcggcggc gcgcgcccgc
    23641 aggatgtcgg tcgcggcggc catctgaaga ccgacttgcg catctatggt gtcgaggttg
    23701 cctacggatt ggtccagata cgcggcggcg ccgagcatca cggagtcgcc gatcgccgtc
    23761 acccgtccca caggagcggc ctcggcctta tcgggagcag tcttctcttt ctcctttgct
    23821 gcggtccccg aagatgaggc cggattcgcc gtctcctttc cggtcttccg ggttcccgcg
    23881 gccttctcgt ggtccccggt ctttttctgc ggatgggtcc gcggttcgct ttgcgtatcc
    23941 accgcctttt gggttgatgg tttctgggga gtcttgtccg aggtgtggat cgccttcatg
    24001 tccaggtagg aaggcttttc cggcggtttc gcctggacga cggcaacgct gagcatcacg
    24061 cttacggcga cactcaccac gcccgctccg gcccaccgga caccgagctg ccagcgacgc
    24121 ccgccctgag cttctcgaag cgccctccag gacctctcca gggcgccctt gcgtatcgga
    24181 gtctcgacga atcggtaaga gaggtcagcg agcgccacgg ctgctgccag gcgcacgacc
    24241 agcagcggcc aaccgtgtat gggcacgtcg atgtccgggc gcgtgatcat gtagaccggc
    24301 cagtgccaca ggtagatgcc gtacgacctg accccgaccc aacgcagcgt ccggcggccc
    24361 agcagcgctc cgcccaggta cgtgcgcggg tgaacggtga ccataatcac cacggcggta
    24421 gccagcccga cgcatgcgaa gccgccccgg tacaagaacg gctggaactc ggtgatgcgc
    24481 aggcagaacc agacgagcac gcccagagcg ccgaggccag cgaggtcgag cagcagcggg
    24541 aagacccatc ccatcccctg ctcgaaagcg cggcggatcg ccgcccgggt atagaaccgt
    24601 cccggctccc acaggaacgc gagcgcagcc ccgatcagga gccccgaagc gcgggtatcg
    24661 gtgccgtagt agatccggga tgggtccacc cccggtttgt acagcacggc catgagcaag
    24721 atagaagcga atgctccgac gataatgaag aagatcatac gatgacgccg gtagcgcatc
    24781 gcgcccacgc ccatcccggc ggcgaacagc aggggccaca gcaagtagaa ttgctcctcg
    24841 accgcgagcg accacagatg cttcaagagc gaaggccggc ccatggcctc gaagtacgac
    24901 tcgtggccta tcaccagata ccagtttgtc acgtagccga acgcggcgag cgcatcgctc
    24961 cgcaaccccg caacctcgcc gggcaggaaa accacggcga aggccagcgt tactacgagt
    25021 aacaggtaga gagcgggcag caaccgccgg gcgcgtctgt accagaagcc cttgaggtct
    25081 atgcggcccc gctggcgcca ctccgcaaga agcagagccg taatgaggta tccactgata
    25141 acgaagaaga cctcgacccc caggaaaccg cccgggatcc aacccaggtc agcatggtag
    25201 agaagcacgg ctatgacggc gaaggcccgt agcccatcga gcccaggcat atgcggcagc
    25261 cgcacgcccc tctttgtttc ggcatcatca tcgatccggg gcgactcggc cagccgaacg
    25321 cggctcgcgt gtatggtcat ctctcgcatc acactcttga tcctggcggg cgaatggtgt
    25381 tccggtatgt gactcatctc tctttgttac tcctaccgcc caaagtaagc agccgttctt
    25441 ccgggattcg cctggatgag atgtttgcca tgcccggtat cccggtccgg ttcccgcccc
    25501 gccacagcgg cgacggcaca ctccggcccg accgggtgct ccagtatgac aaagcacgtc
    25561 cccggagacg gacgccacat ggcgtcagtc ctcctggatc gcaattcggg cacgttctgc
    25621 ccccctccag attgcgaaca tgaatatttt actccccgca ctacgaaaaa ctcaactcct
    25681 gaaccaaagc ctgataaaag ttcctactcg attgtccccg taaaccagaa gaatttggcc
    25741 gatgaaggcg gctaccaggt ccgccgcctg acaccaaact cacctgcgca aatattaatg
    25801 ctcaccgact tattgcaaat cattcccgat aaagaggtct tatctccatc tttgtgtgtt
    25861 acaagcgggt cttgggcgct cttgtttggg aacagctcgc agtaggcgac taccgcagac
    25921 ttacgtaggc gaaaacgcct ataaaaggcc ggtttcgtag atttcaagaa atgttcttgc
    25981 ttgtaatccg atcacattgt tagggctctg gtgctgtgat taaatagcag cgcagtagca
    26041 aagttcttat agagcgaacg gcaggggttc gatgttaaag atagaaagtt tgcgggcgga
    26101 gatagacgga caggagatcc tcaagggtct cgacctggat gtcggcaagg gtgagatcca
    26161 tgcgatcatg ggtcccaacg gttccggcaa gagtacgctc tcgaacgtac tcatgggcca
    26221 tccgcgctat gaggtgacgg ggggctccgt aactttcatg ggcgaggaca tcttcgagtt
    26281 ggaagcggat gagcgcgcca agctcggcat gttcctggct tttcagtacc cgagcgaggt
    26341 tccgggcgtc tccgtggcga acttcctgcg gactgcggtc aactccgtgc gtgaggagga
    26401 tctctctccg atggagatgt accggctcat ccaggagaag atgaagatca tgcagatgga
    26461 ccccaagttc gccgagcggt atctgaacga agggttctcc ggcggcgaga agaagcgcaa
    26521 cgagattctg cagatgctca tgctggagcc gaagctcgcc atcatggatg agactgactc
    26581 cgggctcgac atcgacgcgc ttcaggtggt tgcgaagggc gtcaatgagt tgcgcggacc
    26641 ggacttttcc gcggtcatca tcacgcacta ccagaggatc ctgaggtata tcgagccgga
    26701 tcacgtccac gtcatgctcg atgggcgtat agtgacctcg ggcggcaagg agcttgcgct
    26761 ggcgctggag gacaagggct acgactgggt gcggcaggag ttcggctcgg ccgcttaagt
    26821 taaggaggag taatgcccga agacaagaca attcaggagc tcgggttaga cgagtacaag
    26881 ttcggattcc gcgacccgga ggagtacttc ttcaagaccc cgaggaaggg tatcgatccg
    26941 gagatcgtcg ccatgatctc caagcacaag aacgagccgg agtggatgct ggagttccgg
    27001 ctgaaggcgc ttgagtcgtt cttcgagaag cccaccccga cctggggcgg agatctctcg
    27061 gagatggact tcgacgagat ctactactac atccggccga tggagaatca gggccgctcc
    27121 tgggacgacg tgccggacga catcaagaac acgttcgaga agctgggcat cccgcaggcc
    27181 gagcgcgagt cgctcgccgg cgtcggggcg cagtacgagt ccgaggtcgt ctaccacagc
    27241 ctcaaagagg agtgggagaa gtcgggcgtc atcttcatgg acatggacgg cggtctgcgc
    27301 gagcacgagg acatcgtgcg cgagtacttc ggcaccatca tcccgccgga cgacaacaag
    27361 ttcgcggcgc tcaacagcgc ggtctggtcg ggcggctcgt tcgtgtacgt cccgccgggc
    27421 gtccacatag acatcccctt gcaggcgtac ttccgcatca acgcggagaa catgggccag
    27481 ttcgagcgga cgctcatcat cgccgacgag ggctcttacg tccactacat cgagggctgc
    27541 accgccccga cgtacacctc gaactcgctg cactcggcgg ttgtcgagct catagcgaag
    27601 ccgaacgcgc gcatccgcta ctcgac
//
LOCUS       DASSMB010000019         8437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1524819, whole genome shotgun sequence.
ACCESSION   DASSMB010000019 DASSMB010000000
VERSION     DASSMB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8437
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524819"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VFJ72_01590"
     CDS             <1..186
                     /locus_tag="VFJ72_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7478186.1"
                     /translation="ELPQRLAELDQSENFAIHCKTGGRSAKAVKLLQDAGFTNVYNVK
                     GGITAWSEEIDPSIPKY"
     gene            283..1350
                     /locus_tag="VFJ72_01595"
     CDS             283..1350
                     /locus_tag="VFJ72_01595"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7478187.1"
                     /translation="MSRIPIFDGHNDVLLKLLMPEPGKERGFFERSDTGHIDLPRTRE
                     GGFGGGFFACWAPPDPEGGWTEESALIVTEDGYEVADAPPLDPNYARSIVEEMTKLLF
                     SLEAESEQRVKVVRTVDALESCLRSGVLAAVLHFEGAENLGPDPGTLETYYESGLRSL
                     GLVWSRPNAYAHGVPFRFPASPDTGPGLTDAGKRLVRECNRLGVLVDLSHLNERGFWD
                     VVQLTDAPLVATHSNAHALCPVTRNLTDRQLDAIRDSDGMVGVNFAVAFLREDGAEDE
                     DTPLDTVVRHVDYLVERIGIDRVGFGSDFDGAMVPKGIGDVSGLPNLMDALRERGYDE
                     AALKKLAHENWLRVLRKTWRD"
     gene            1388..2083
                     /locus_tag="VFJ72_01600"
     CDS             1388..2083
                     /locus_tag="VFJ72_01600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7478188.1"
                     /translation="MSATKPSVRDTDTPGNVRAQRRGMMPTVLLLCAALVAAAFVVFR
                     TTEPEGTPVARFPLPERYADSYSNDWGAARVQGAHEGTDLFAPRGTPIRSITAGTVSR
                     AWGSSGNGWNTLGGYTVMIEAAYDMGPIERGDRLYYAHMDAPTELKPGDIVEAGQKIG
                     EVGDTGQGPEGTRSNFEPHLHLGWYEGWSLLSAERSTVSSGAINPYPLLRWIRHGENL
                     RPGPEAVQNEDGA"
     gene            2097..2753
                     /locus_tag="VFJ72_01605"
     CDS             2097..2753
                     /locus_tag="VFJ72_01605"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET7478189.1"
                     /translation="MDTSERRDNDTGRIEAFSDGVFAIAITLLIIEIGVPHVEGEESL
                     VDSLAGLWPSYLGYFISFLVIGTVWANHHNRFRFITRSDHVLLFLNVLFLMCVAFLPF
                     PTALLAEYLPGTEAHRTTAVAVYAGTLAVTAVFFTALWLYAAGNYRLLDRGMNPELLR
                     AMTRRFVVGMLLYIAAFALAFVSTTLALAIIVGLALLFVLPEPGVRVATPRRARRRAN
                     "
     gene            complement(2756..3676)
                     /locus_tag="VFJ72_01610"
     CDS             complement(2756..3676)
                     /locus_tag="VFJ72_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7478190.1"
                     /translation="MSAEGPIESPAANAPERRRASAWVEVSLLLTVFFFGTNFTAVKI
                     VVESVPPILFAAARFTLAGLLLFLLSRIVEPGNRLKRSDFLPILGLGVVGVTLTQTVF
                     TIGVSLTTAASTALVYSTAPVWGMLLGFALGLERPRLTGILGICLSLVGVGFIVYGGL
                     EFAGTSLVGDALILAAAVFWGSYTVLSLGLLRRYPPLTLAAYAMTLGGLVAFPLAAVE
                     GGGLDLGAISSLTWGAAVYSTLCSSAFGFAAWGWGISHVGANRVLIYQYLVTLTGVSA
                     GILLLGEDFGLGQLVGAAVIIAGVYLARLR"
     gene            complement(3698..4756)
                     /locus_tag="VFJ72_01615"
     CDS             complement(3698..4756)
                     /locus_tag="VFJ72_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET7478191.1"
                     /translation="MRDASYEFLKKLLSTPGPSGYEAAAAAVWREEATQFAGVRGDRM
                     GNSFATLNAGGSPRVMLAGHIDEIGVMVTHIDDQGLLRFTGVGGWDPQVLVGQRIRLQ
                     TKTGEVMGVIGKKAIHVMEAEERKKVSEIKNLWIDIGAKDGDEAKGMVRVGDVGVLDQ
                     DLVELPNGRIASRSVDNRMGAFVVLEALRLLSEEENLNAEVVAVACVQEEIGLYGARG
                     AAFGLDPDAAIAVDVTHATDTPGVSKNENGDHALGSGPVIKRASNLSPIVSEGLIAAA
                     EAENIAYSLEADSRSTGTDADAIQFTRAGIATSVISCPNRYMHSPNEVVDLQDVENVA
                     RVISAYIKTLDADTDFVR"
     gene            complement(4809..5507)
                     /locus_tag="VFJ72_01620"
     CDS             complement(4809..5507)
                     /locus_tag="VFJ72_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HET7478192.1"
                     /translation="MIVRERQDSFLLFGQHEHALVSAEFARWLNVEITARDATLLAIA
                     NHDLAWRPLDRDVLWDEERDKPYSFVDYPVEQKLHAYTAGLDELEGSSSYAACLCSMH
                     YETLVRSFGMSEAEARFAEEEARRQERLREGMSHEESESLERNLRLLKLCDGLSLFVC
                     LNEPGRSDRPPPYPGGFELWGNVYEPVWEDERTFRLEPNPFTDEFEVELTYREVGRDR
                     RLLGSSVLRVRVTC"
     gene            complement(5560..6099)
                     /locus_tag="VFJ72_01625"
     CDS             complement(5560..6099)
                     /locus_tag="VFJ72_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478193.1"
                     /translation="MSIPRYYTIGAVARDARSLAALGERLDESSVTLDSLMVLSRRRD
                     EALARNILPGARVEKVEYGLTRMQWIEFASAFLGVISVSVLMGAVHLLTGIVVQAVMT
                     VAAVTGLVIYHRRPQLSKKLGVMALPESFVGEWEERFESGFAVVLATVREDEFDAAQE
                     AFLAEGIEAPLAVDRRPVL"
     gene            complement(6144..6698)
                     /locus_tag="VFJ72_01630"
     CDS             complement(6144..6698)
                     /locus_tag="VFJ72_01630"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7478194.1"
                     /translation="MKERTRKIRDFDRALFGAIFRVKWAPLTTVMRTLTITGTAGALW
                     GFIAFAAFLLTGLDLTRLLVPWATIAGSWVLAEGTKYLFNRARPYISDTEIAPLIKTP
                     SSSSFPSGHSATAAAGALTLSVLYPVFAPVILLAGLLTALSRIYLGVHYPLDVLAGML
                     IGAATSGVVLLLAPLARTLALTLA"
     gene            6880..7146
                     /locus_tag="VFJ72_01635"
     CDS             6880..7146
                     /locus_tag="VFJ72_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478195.1"
                     /translation="MNRDLISKAVEEAKGEEANLVIWDRRDTFTIESGDVKDLDLADD
                     YLTVKMEDGKVTVYVEYGSIYKLVVERERKSRSGIRAGFGAAQS"
     gene            complement(7161..7856)
                     /gene="rpiA"
                     /locus_tag="VFJ72_01640"
     CDS             complement(7161..7856)
                     /gene="rpiA"
                     /locus_tag="VFJ72_01640"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET7478196.1"
                     /translation="MGEIGDLKRIAAYEAVDSNVRSGMTVGLGTGSTASHAVRRVGEL
                     LSGGELSGVRGIPTSERTAALAAEVGIPLVTLAEARPDLTIDGADEISPSLDVIKGYG
                     GALLREKIVAASGREGLVIVADASKLKDPLGDGPLPVEVEPFGWQATFDALASLGCEP
                     KLRTDSQDPSRPFVTDGGHYTVDCLFPSITEPAALETEIKRIPGALECGLFVGIAYAA
                     YVAHEDRTEVLEA"
     gene            complement(7952..8155)
                     /locus_tag="VFJ72_01645"
     CDS             complement(7952..8155)
                     /locus_tag="VFJ72_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET7478197.1"
                     /translation="MARGRVKWFSDEKGYGFIEAEGGEDLFVHYSEVVGEGFRTLQEG
                     SEVEFDVIDGRKGKQASRVEVIG"
BASE COUNT         1522 a         2772 c         2738 g         1405 t
ORIGIN      
        1 gagcttccgc agcgtctggc ggagcttgac cagagcgaga acttcgccat ccactgcaag
       61 accggcggac gcagcgcgaa ggccgtgaag ctcttgcagg acgcgggctt tacgaacgtc
      121 tacaacgtca agggcggcat caccgcctgg agcgaggaga tcgacccgag catcccgaag
      181 tactaacctg tagataacct ttacgagcgg gaccgtaatc tttacggtcc cgctctttcg
      241 ttcccgcagc gtccgcataa cccagcctgt atcatcatcc gaatgagtcg gataccgatc
      301 ttcgacggcc acaacgacgt cttgctgaaa ctcctgatgc ccgagcccgg taaagagcgc
      361 ggcttcttcg agcgaagtga tacgggacat atagacctgc cacggacgcg cgagggtggc
      421 ttcggcggcg gcttcttcgc ctgctgggct ccgccggacc ccgagggtgg atggaccgag
      481 gagtccgcgc tcatcgtcac tgaagacggc tacgaggtcg cggacgcgcc gccgctggac
      541 ccgaactacg cgcgaagcat tgtggaggag atgacgaaac tcctgttcag tcttgaagcg
      601 gagtcagagc agcgggtgaa ggtcgtgcgg acggtggatg ctcttgaatc ctgcctgcgg
      661 agcggagtgc tggctgccgt cctgcacttc gagggcgcgg agaacctggg cccggacccc
      721 ggaacgctgg agacttatta tgagtcgggc ctgcgctctc tcggcctcgt ctggagccgt
      781 ccgaacgcct acgcgcacgg cgtgccgttc aggttcccgg cctcgccgga taccggcccc
      841 ggactcacgg atgccgggaa gaggctcgtc cgcgagtgca accggctcgg tgtgctggta
      901 gacctctcgc acctgaacga gcgcggcttc tgggacgtgg tgcaacttac cgatgctcca
      961 ctcgtggcga cgcactcgaa cgcccacgcg ctctgtcccg tgacgcgcaa cctgaccgac
     1021 cggcagctag acgccatacg ggactcggat gggatggtgg gcgtgaactt cgccgtcgcg
     1081 ttcctgcgcg aggacggcgc cgaggacgag gacacgccgc tcgataccgt ggtgaggcac
     1141 gtggactacc tggtagagcg gatcggcata gatcgcgtcg gattcgggtc ggacttcgac
     1201 ggggccatgg tcccgaaggg gatcggggac gtctccgggc tcccgaacct catggacgcg
     1261 ctgcgcgagc gcggctacga cgaggctgct ctgaagaagc tcgcgcacga gaactggctg
     1321 cgggtactgc gcaagacctg gcgcgattag cacccgaccg aagctcccgg caggttaaac
     1381 tcacgccatg agcgcaacga agccgtccgt aagggacacg gatacccccg gcaatgtccg
     1441 cgctcagcgc cggggcatga tgccgacggt attgctcttg tgtgccgcgc tcgtagctgc
     1501 ggcgttcgtg gtgtttcgca ctacggagcc cgaaggaacg ccggtcgcca ggtttccgtt
     1561 gccggagcgg tacgcggatt cgtactcgaa cgactgggga gcggcgcggg tgcagggcgc
     1621 gcacgagggc acagatctct tcgcgccgcg aggcacgccc atccgttcaa ttaccgccgg
     1681 tacggtgagc cgggcgtggg gctcttcggg gaacggatgg aataccctgg gcggttacac
     1741 ggtcatgatc gaggcggctt acgacatggg accgatagag cgcggcgacc gactgtacta
     1801 cgcgcatatg gacgctccaa cagagctgaa gcccggcgat attgtcgaag ccgggcagaa
     1861 gatcggggag gtcggggata caggacaggg gccggaaggg acgcgaagca acttcgagcc
     1921 gcacctgcac ctcggctggt acgagggatg gagccttctg agtgcggaac gctccacggt
     1981 ttcgagcggc gcgatcaacc cgtatccgct gctgcgctgg atccggcacg gtgagaattt
     2041 gcgtccggga ccggaagcag ttcagaatga ggatggtgcg tagacaggag tagagcttgg
     2101 atacgagcga gcgtcgggac aacgataccg gcaggatcga agcgttctcg gacggggtgt
     2161 tcgccatcgc gatcacgctg ctcatcatcg agatcggggt ccctcacgta gagggcgagg
     2221 agtcgctcgt ggacagtctc gccggactgt ggccctctta cctcggctac ttcatcagct
     2281 tcctggtcat cgggaccgtc tgggcgaacc atcacaaccg cttccgcttc atcacccgtt
     2341 cggaccacgt cctgctcttt ctgaacgtcc tcttcctgat gtgcgtcgcc ttcctgccct
     2401 tcccaaccgc cctgctcgcg gagtacctgc cggggacgga ggcccatcgc accaccgccg
     2461 tagccgtcta cgccggcacg ctcgctgtaa cagccgtctt cttcaccgct ctatggctct
     2521 acgcagcggg caactaccgt ctgctggatc gcggtatgaa cccggaactg ctgcgcgcga
     2581 tgacgcggcg tttcgtggtc gggatgctgc tctacatagc cgccttcgcg ctcgccttcg
     2641 tcagcacgac gctggcgctc gcgatcatcg tcggcctggc gctgcttttc gtgctgccgg
     2701 aaccgggagt tcgggtggct acacctcgcc gcgctcgccg acgcgcgaac tagggctacc
     2761 tgagacgcgc cagatagacg ccggcgatga tgaccgccgc tcctactagc tgtccaagcc
     2821 cgaaatcttc ccccaggagc aggatgccgg cgctcacgcc ggtcagggtg acgaggtact
     2881 ggtagatgag gacgcggttg gcgccgacat ggctgatacc ccatccccag gccgcgaacc
     2941 cgaatgccga ggaacagagc gtggagtaga ccgcggcgcc ccaggtcagg gaactgatgg
     3001 cgccgaggtc cagaccgccg ccctcgacag cggccagcgg aaaggcgacg agtccgccga
     3061 gcgtcatggc ataggcggcg agcgtgagcg gcgggtaacg gcggaggagt ccgagggaca
     3121 gaaccgtgta tgagccccag aagacggcgg cggcgaggat gagcgcgtct ccgacgaggc
     3181 tcgttccggc gaactccagg ccgccgtaga cgatgaagcc aaccccgacg agcgagaggc
     3241 agatgccgag gatgccggtc agccgcgggc gctccagacc tagcgcgaaa ccgagcagca
     3301 tcccccacac cggcgcggtg gagtagacga gcgcggtgct ggccgccgtg gtgaggctca
     3361 cgccgatggt gaagaccgtc tgggtgaggg tgacgccgac gacgccgagg ccgaggatgg
     3421 gtaggaagtc gctccgcttg agccggttcc ccggctccac gatacgcgag agcaggaaga
     3481 gcaggagccc ggccagggtg aagcgcgccg ccgcgaagag gatgggcggc acgctctcga
     3541 ccacgatctt gaccgccgtg aagttcgtgc cgaagaagaa gaccgtcagg agcagcgaga
     3601 cctcgaccca ggccgaggct cgccgtctct ccggcgcgtt tgcggcagga ctctcgatgg
     3661 gcccttcggc ggacactacg ggcgcccgtt aggcatgcta gcggacgaaa tccgtgtcgg
     3721 cgtccagggt cttgatgtag gcggagatca cacgcgcgac gttctccacg tcctggaggt
     3781 ccacgacctc gttcggcgag tgcatgtagc ggttgggaca cgagatcaca ctcgtggcga
     3841 tacccgcccg cgtgaactgt atggcgtccg cgtccgtgcc ggtcgaacgg gaatcggcct
     3901 caaggctgta ggcgatgttc tcggcctcgg cggcggcgat gagcccctcg gagacgatag
     3961 gcgagaggtt cgaggcccgc tttatgacgg gtccgctccc cagcgcgtgg tcgccgttct
     4021 cgttcttgga gacgccgggc gtgtcggtgg cgtgggtgac gtccacggct atcgcggcgt
     4081 ccgggtcgag gccgaaggcg gccccgcggg cgccgtacag cccgatctcc tcctgcacgc
     4141 acgcgaccgc gacgacctcg gcgttcaggt tttcctcttc ggagagcagg cgcagggctt
     4201 cgagcacgac gaacgcgccc atgcggttat ccacggagcg ggaggcgatg cggccgttcg
     4261 gcagctcgac taggtcctgg tccagcacac ccacgtcccc gacacggacc atgcccttcg
     4321 cctcgtcgcc gtccttcgcg ccgatatcga tccagaggtt cttaatctcc gagaccttct
     4381 tgcgctcctc ggcctccatg acgtgtatcg ccttcttgcc gataacgccc ataacttcgc
     4441 cagtcttcgt ctgcagccgt atccgctgcc ccaccaggac ctgcgggtcc cagcctccga
     4501 cgcccgtgaa gcgcagtagc ccctgatcat cgatgtgggt gaccatcacc ccgatctcat
     4561 caatgtgccc ggcgagcatg acccgcggcg aaccgcccgc gttcagagtg gcgaaggagt
     4621 tacccatccg gtcgccccgg actccggcga attgcgtcgc ctcctcccgc cagaccgcgg
     4681 cggcggctgc ctcgtaaccg ctcggtcccg gcgtggacaa tagcttcttc aagaactcgt
     4741 aagatgcgtc tctcacagta ttcctttcta aatatggaga aggtattaaa agcggcgccc
     4801 tccggcgcct aacaggttac ccgaactcgc aggacgctgc tcccgagcag ccgccggtcc
     4861 cgcccgacct cccggtacgt cagctcgacc tcgaactcat ccgtaaacgg gttgggctcc
     4921 agacggaacg tccgctcgtc ctcccacacc ggctcgtaga cgttccccca cagctcgaac
     4981 cctccaggat acggcggcgg acggtccgaa cggcccggtt cgttcaggca cacgaacagc
     5041 gatagcccgt cacagagctt gagcagacgc aggttgcgct ccagactctc gctctcctca
     5101 tgggacatcc cctcccgcaa ccgctcctgc cgccgggcct cctcctccgc gaaccgcgcc
     5161 tcagcctcgc tcatcccgaa gctgcgcacg agagtctcgt agtgcatgct gcacaggcag
     5221 gccgcgtagg agctgcttcc ctccaactcg tccaggccag ccgtgtaagc gtggagcttc
     5281 tgctcgaccg gatagtccac gaacgagtag ggtttgtcgc gttcctcgtc ccacaggaca
     5341 tcccggtcca gcggacgcca cgcgaggtca tggttggcga tggcgaggag cgtggcgtcg
     5401 cgggctgtga tctcgacgtt cagccatcgg gcgaactcgg cagagaccag cgcgtgctcg
     5461 tgctgcccga agagcaaaaa agagtcctgt cgctcgcgaa cgatcacccg tagagcttac
     5521 cagccaaata gccgttgagt tttcaatccc ctggcctcgc tagagcaccg gccggcggtc
     5581 cacggcgagc ggcgcttcga tgccctccgc gaggaacgcc tcctgggccg cgtcgaactc
     5641 gtcctcgcgc accgtggcga ggaccacggc gaacccggac tcgaacctct cctcccactc
     5701 cccgacgaag ctctccggca gcgccattac cccgagcttc ttcgacaact gcggccgccg
     5761 atgatagata acgagcccgg taacggccgc caccgtcatg accgcctgca ctacgatacc
     5821 tgtgaggaga tggacggcgc ccatcaggac gctcaccgat atgacgccca gaaacgcgct
     5881 cgcgaactct atccactgca tccgtgtgag gccgtactcc accttctcca cccgcgcgcc
     5941 tggcagtatg ttccgcgcca atgcctcgtc ccgccgccgc gagaggacca tcagagagtc
     6001 gagggttacg ctcgactcat ccagccgctc cccgagggcc gcgagcgagc gagcatcgcg
     6061 ggcgacggct ccgatggtgt agtagcgggg aatgctcatg cttactttct aacccacgcg
     6121 gagcatagag ccggtaagcg gcttcacgcc agcgtcagtg caagcgtccg agccaggggc
     6181 gcgagcagca ggacgacccc ggaggtcgcg gcgccgataa gcatgccggc cagcacatct
     6241 agcgggtagt ggacgccgag gtagatcctg gacagcgcgg tcagaaggcc ggctagcagg
     6301 atcaccggcg cgaagaccgg gtagaggacc gagagcgtca gggctccggc ggcggcggtc
     6361 gcggagtgtc cggaggggaa ggaactggag gagggcgtct ttatcagggg ggctatctcg
     6421 gtgtcggaga tgtagggccg ggcgcggttg aagaggtact tggtcccctc ggcgagtacc
     6481 caggagccgg ctatcgtggc ccaggggacc aggagccggg tgaggtccag gccggtcagg
     6541 aggaaggcgg cgaaggctat gaagccccac agcgcgccag ccgtcccggt gatggtcagg
     6601 gtccgcatca cggtggtgag cggcgcccac ttgacgcgga agatcgcccc gaacagcgcc
     6661 cggtcgaagt ctcttatctt gcgcgtgcgt tccttcaagc tgctccgtag ttcaagatgc
     6721 aggttcctct actcgacggc tgcgtatttt acaccccgct tcccgtaacg accggtgaga
     6781 gtccgtgccg tctcgtgcgg cacgggtacc cctgtaccgg ccggtatcta aagtaaaatg
     6841 ggacggatta ctatcagaga gatattagag gagcgtgtaa tgaaccggga tttgatctcg
     6901 aaggccgtgg aagaggcgaa gggcgaggag gcgaacctcg tcatctggga ccgccgcgac
     6961 actttcacca ttgagtccgg cgacgtgaag gacctggacc tcgccgacga ctacctcacg
     7021 gtgaagatgg aagacggcaa ggtaaccgtc tacgtggagt acggctccat ctacaagctg
     7081 gtcgtggagc gggagcgcaa gtcccgctcc ggcatccgcg ccggctttgg cgccgcgcag
     7141 agctagctcc ccgtcaggag ctacgcctcc agcacctccg tccggtcctc gtgggccacg
     7201 taggcggcgt aggctatccc gacgaacagg ccgcactcca gagcgccggg gattcgcttt
     7261 atctcggtct ccagcgcggc cggttcggtt atggagggga agaggcagtc aacggtgtag
     7321 tgcccgccgt cggtgacgaa ggggcgggag gggtcctggc tgtctgtgcg gagcttcggt
     7381 tcgcagccca gggaagccag ggcgtcgaac gtggcctgcc acccgaaggg ctcgacttct
     7441 accgggagcg ggccgtcgcc cagggggtct ttcagcttcg aggcgtcggc gacgatcacg
     7501 agcccctccc ggccggaggc ggcgacgatc ttctctcgca agagcgcccc accgtagccc
     7561 ttgatcacgt cgagggacgg ggagatctca tccgccccgt ctatggtcag gtccgggcgc
     7621 gcctcggcga gagtgacgag tggaatcccg acctcggcgg cgagcgccgc cgtccgctcg
     7681 gaggtcggta tcccccgcac gccggagagt tcgccgccgc tcaacagctc cccgaccctg
     7741 cgtaccgcgt ggctggccgt cgagcccgtg ccgagtccga ccgtcatccc gctccgcacg
     7801 ttcgagtcca cggcctcgta agcggcgatt cgtttgaggt ccccgatctc tcccaccaaa
     7861 ccacctccag agataccaaa gataggagaa gccgggcact cgaaagtccc ggctccttgt
     7921 cagttctcgg ctgactggct tcgccggccc gctaccctat cacctcgacc ctcgaggcct
     7981 gcttgccctt ccggccatct atcacgtcga actcgacctc gctgccctcc tgcagggtac
     8041 ggaagccctc accgactacc tcggagtagt ggacgaagag gtcctctccc ccctcggcct
     8101 cgataaagcc gtaacccttc tcgtcggaga accacttgac tctgcccctc gccacagtac
     8161 ctccagcgtt cttctccggc aagaccgcgt gccgccgata tcccggatct ccttcacatc
     8221 acgccgccag gtaagatgcc cgatcgacag gtcgagactc taccaccacg cctcatccga
     8281 agcaaacgcg ccagacgacg gggaacgagc cggatcaggc gcgggagcgc aatccgtcca
     8341 gagtgaccct gacgagccgt cggaccgcgg cttcggacgg caggctgccg gccgctgtga
     8401 gggcatggag acagtagctc gcgagctcgt caggcgc
//
LOCUS       DASSMB010000020        16357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_384325, whole genome shotgun sequence.
ACCESSION   DASSMB010000020 DASSMB010000000
VERSION     DASSMB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16357
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384325"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFJ72_01650"
     CDS             <1..253
                     /locus_tag="VFJ72_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7478198.1"
                     /translation="ITLGPAGALVADRETVTHVPAPEVEVVDTTGAGDAFVGALVARL
                     ARGDALPDAAAYAVRAGAAAVTKEGAQGALPTPEMVEAL"
     gene            250..516
                     /locus_tag="VFJ72_01655"
     CDS             250..516
                     /locus_tag="VFJ72_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HET7478199.1"
                     /translation="MKRGGILNARLSAALARLGHTDLVVACDAGLTIPFVPEVHDLLR
                     EGLLDPALAPREKLKRMVAGTKLVVRAGGATPYSNVILRCGVPF"
     gene            803..2611
                     /locus_tag="VFJ72_01660"
     CDS             803..2611
                     /locus_tag="VFJ72_01660"
                     /inference="COORDINATES: protein motif:HMM:NF011663.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HET7478200.1"
                     /translation="MRVQRFGSYLSGMVMPNIGAFIAWGLITALFIPDGWLPNEHLAK
                     LVEPMILYLLPLLIGYTGGRMVYDVRGGVVGAIATMGAIVGSDIPMLLGALIMGPLGG
                     YVIKKFDDLVQHRVRQGFEMLVSTFSAGILGGILAIIGVLGAGPLITSVSNTLGAGVD
                     FIVSLGLLPLVSIIVEPAKILFLNNAINHGILAPLAIEQARQAGKSILFMVETNPGPG
                     FGILLAYSVFGRGISKRSAPGAAILQVLGGIHEIYFPYVLMKPLLVLAAIAGGMSGLF
                     TATLFKVGLVSTPAPGSIFAYAAMTPRGNFVGVFADVLVATVVSFLVASVIIRRSSDI
                     ESDDEDESSLAMAAQQMETMKGKKSSVAGTLTSGAGTDERTGTNGHTETADKIAHAKS
                     IVFACDAGMGSSAMGASLLKNKVKKAGMDDISVTNASINNLTDEPDIVVTHKDLTDRA
                     RAKAPSAAHVSVDNFLGSPKYDELVEQIRQGRENGGASTGTADGTTAGTSSGSFDYAG
                     VRKIIFACDAGMGSSAMGASLLRKKANEAGLSEIEIKNASINQIPGDADVIITHRDLT
                     GRAEEKMPEAHHVSVDNFLESPKYDELIEEISTARV"
     gene            2628..4733
                     /locus_tag="VFJ72_01665"
     CDS             2628..4733
                     /locus_tag="VFJ72_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET7478201.1"
                     /translation="MGLSDREILLIEVLLRHPYGLTAEGIAERLGVSARTVHRELQPA
                     SEFLDSHGLTLLRQAGRGVSVEGAPQARERALSALREMGSVGLTPEERRLSLLCTMLA
                     ADQPIKLRALASGLDVAVGTVSRDLDEVEEWLTGFRLSLLRKRGYGVEVVGDEGDRRR
                     AMSRLIFENLDESRLFLRPEDLREEDSTPVREPGRVADQLMGMIGEDRLRRVEALTEN
                     VVASLPYSIADSAFVSMSVHVALMVERRLQGGEIEMDEEILQRLRNTEEYEYARRLAA
                     DIEEDFRVAVPEEEVGYLTMHLRGTKLREDDALRDYFETSDLEVASQVKALIRYVEEQ
                     TGISLAGDSSLYTGLLAHLERAIHRLREHLGIHNPLLSEVKEDYPALFDLVSQAMDKV
                     FVQEKVPEEEVGFVAMHFGAALDRGQGNFPRSVLVICSSGIGTSKILASRLGKSFPQI
                     QTIRDSSLFELDSLNIEDFDLVVSTVPLPLPDRSYVQVRPFLTDDEVERIRNHLLEKS
                     LDARLANRAAFESLEVFGGGHAKFHQMAEATQVIAELVDDAYLAHHEAGGSLEVAVRR
                     MCASLAGKDLVSDPRSLEESLLSRMELGGIGIPGTVLALFHARDAAAVRPSFSVHDFD
                     EPLELEGLDGAMMRVRRSLLMVAPLTLSPVALEAISEISAAMVEQPVEREVFEDGSEA
                     RIIEVLQNIFARFLHNKLS"
     gene            4759..5193
                     /locus_tag="VFJ72_01670"
     CDS             4759..5193
                     /locus_tag="VFJ72_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7478202.1"
                     /translation="MSEILTPATIELKANLESQEEAIRRAGKLLVDNGNVDERYIDSM
                     FEREKSVSTFMGNAVAIPHGTNDSKQWVERSGLSVITVPEGVEYGDGDIAMLIIGIAG
                     KGDEHLEMLSKIAIVCSEEENVARIVMSESKEELLSIFDEVM"
     gene            5194..6366
                     /locus_tag="VFJ72_01675"
     CDS             5194..6366
                     /locus_tag="VFJ72_01675"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002649.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HET7478203.1"
                     /translation="MLGVHFGAGNIGRGFIGQLLHESGYDVSFVDVQAQIVEALKTQG
                     RYEVILAAEGEERVLVDRVTAFHSIDEAEEVVRQIARADLVTTAVGPPVLPILAPAIA
                     NGLMERARSGGGPLNVIACENMIGGSESLRSSVMEHLSGEDAEAVAGIAGFPNAAVDR
                     IVPEQTTGGIDVLVEPFFEWVVDASQVKGDRPDVSGITYVESLAPYIERKLLTVNTGH
                     AAVAYLGYARGRATISDALEDDAVYEEASGALEETGRLMVEEHGFDEEEHRKYREKTL
                     VRFRNPRITDAVTRVARTPIRKLGRNERLVSPALRLLDRGYTPVHLATVIGAVLRYDN
                     AEDEQAAELQEKVRAEGERAALASCAGIEEDHPLVDLVAERLGSSREFSHQRGGGA"
     gene            6500..9154
                     /gene="ppdK"
                     /locus_tag="VFJ72_01680"
     CDS             6500..9154
                     /gene="ppdK"
                     /locus_tag="VFJ72_01680"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET7478204.1"
                     /translation="MASEVYVYEFSEGSGEMKDLLGGKGAGLAAMSGLGLPVPPGFTI
                     TTEACRAYMRAGEPPAELAEQVDAHLGELEESTGKRLGDPENPLLVSVRSGAPVSMPG
                     MMDTVLNLGLSDAAVDGFARRTGDGRFAHDSYRRFIQMFGDVVLKVDHEKFEKALETL
                     KEERGVEDDTQLSEEDLKALISTYKQIVEEETGSSFPEDPREQLDLAIRAVFDSWNNP
                     RANAYRKEFGLPDDLGTAVTVQAMVFGNMGDDSATGVVFTRDPSTGEQGLFGEFLLNA
                     QGEDVVAGIRTPRPISEMQEDLPHAFEELLETMQRLENEYRDMQDIEFTVERDRLFML
                     QTRSGKRTAVAALRIAYEMAEEGIISREEAVMRVEPRQLDQVLHPYIDPEADLDVLAV
                     GLPASPGAATGSVVFTADEAEERGEAGEAVILVRKETTPDDVHGMMRAKGTLTALGGM
                     TSHAAVVARGLGIPAVTGCKALKIDAAAQTFSIDGQTFRVGDTITIEGSTGKVVAGEA
                     PLVEPELSEDFERILAWADEFRTLGVRANADTPQDARKARQLGAEGIGLCRTEHMFME
                     EGRLEIMREMILSETEEAETQALIRLEPLQRGDFEGIFEAMNGLPVTVRLLDPPLHEF
                     LPDSRDLYERLAELGDEESEEEKEIRRQLRVVESLEEANPMLGLRGCRLGIVRPGLYR
                     MQARAIAEAAKAVREKGGSVEVEIMVPLVGFRTELAETRRDIEDVVNEVLGDANPVRI
                     GTMIELPRACAVAGDIANEADFFSFGTNDLTQTTCGLSRDDAESGFLARYLQEGILEK
                     NPFETIDRDGVGELVAMARERGRETNPDLKLGICGEHGGDPESIQFFHETGLDYVSCS
                     PYRVPVARLAAAQTTLRERGSVAQASAG"
     gene            complement(9205..10035)
                     /locus_tag="VFJ72_01685"
     CDS             complement(9205..10035)
                     /locus_tag="VFJ72_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7478205.1"
                     /translation="MFRKDLLPIIRVGPSREDRLTLVEGTEEDGWILHGTLRSQQTGE
                     TFTVRGGYLDLLKRTSGAYNIANLTNFLPGAGRGYEPLWRTRSLTLLTGEPFPNEREL
                     EIISGLVNLERGGRYLDLGCSAGLYTRSLARELDGNGDVVGVDISPSMLREAARRIRA
                     AGAARASLARADAENLPFADGSFAGAVCGGSLNEFGDPARALRETRRVLEPGGRIAIM
                     GILRARTSRGRRLQRFLSTSGIKFFVPDEIWSMLAHAGFDPDPLEVHGSVFFAGANRR
                     "
     gene            complement(10035..10679)
                     /locus_tag="VFJ72_01690"
     CDS             complement(10035..10679)
                     /locus_tag="VFJ72_01690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7478206.1"
                     /translation="MPPEKPHDIEREYPWEVQGANADALRSAMSHFPTGVTVVTSGRD
                     ERAEGMTANAVISVSLDPLLFLVSVHKAARLNPRIKEEKTFAVSVLADDQEGLSRLFS
                     SPERSSGLHAVHSLGGGYGTVGAPLAAGAMAVIECELEAVYAGGDHDLFLGRVVEVRL
                     GDTRKGPLVFHEGGYPTLKPAAAPGESGAFVDSVRGFDARMGRLKDRRRDRRRG"
     gene            complement(10718..11434)
                     /locus_tag="VFJ72_01695"
     CDS             complement(10718..11434)
                     /locus_tag="VFJ72_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478207.1"
                     /translation="MARGPIVIVGGVTSWPWRYKDLARTLHELSGAEVHVANITPFDW
                     LLGRIRGFGQLVFQIATAVDRALLESESKKAVLVGHSAGGVACRVYLGGDPPYGGRRY
                     SGHRRVSHLITLGSPHNVPDRGLLAPVARVNDLFPGALHAPAGLSYLSVAGNAVDGNS
                     SARARKRYERFIEDGRIAGDGVVPVEAALLPGAGSVVLDGVYHGPPFGRWYGSDLETV
                     ERWWPRELRVVPPLVEEHRA"
     gene            complement(11475..12089)
                     /locus_tag="VFJ72_01700"
     CDS             complement(11475..12089)
                     /locus_tag="VFJ72_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HET7478208.1"
                     /translation="MFEFVFNAFVTLLVVIDPLGMAPIFAALTNGFSEERKRESAIRG
                     TFLGAAILFVFALVGNTLLHALGIGLPAFRIAGGILLFLLSLDMIFARPSGMRSSTVR
                     EQEEESYQQDISVFPLAIPLLAGPGGITTVLLYTGGGPEQIALFLAVLLVVLLLTLIS
                     LLIAPRIMRLFGETGANVLSRVLGVLLAALAVQFVLDGIHISLT"
     gene            complement(12144..12665)
                     /locus_tag="VFJ72_01705"
     CDS             complement(12144..12665)
                     /locus_tag="VFJ72_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HET7478209.1"
                     /translation="MPTKPPVVWYPAHPNNYTGANRPKSDRITKIIIHVTQGSWSSAV
                     NWFQNPSAGVSAHYTVRSADGKIAQSVSDLNIAYHCGYWPYNVHSIGIEHEGFIDDPK
                     WFTKEMYRSSAKLTAYLCRHYGIPINRNHIIGHNEVPGCPGPGGGAGCHMDPGQWWWW
                     SYYMKRVRRFANR"
     gene            complement(12665..13297)
                     /locus_tag="VFJ72_01710"
     CDS             complement(12665..13297)
                     /locus_tag="VFJ72_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET7478210.1"
                     /translation="MAIDRRTFLKLSATGLAGVALLGNAVTGQAQTAQKPGSGLMKEF
                     DTAAKEYGVPVGVLLAMGHVNTRWEMPAPDSNKYEKGDLHGWGSYGIMALVQNPTSDT
                     LGEASRLTGISEDKLKTDRASNIKGGAALLAASIGRQKPQELRGFFGAVAGRGKASGK
                     NYKAVAGVGGGDLYAEQVFDTLKRGASEKTKSGEKISLAARSLDERIGGR"
     gene            complement(13440..14177)
                     /locus_tag="VFJ72_01715"
     CDS             complement(13440..14177)
                     /locus_tag="VFJ72_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478211.1"
                     /translation="MEILTARPFENPEDGFGLQQAVLEETSRGERGPTALMWSSSRYI
                     GATRPETRLPGFGEATRVAGESGFPVLVRNSGGGAVAANRGSLSFSLTFPVDDLRHGL
                     YERYSEGVDLVAAALRRVGVAAEGGEVEGEFCPGAYSVRSGGPLGVKHAGLAQRVTRR
                     AARLEALILVRDTAEIRAVLESFYGALGLPFRTESVADIPASVGQVARALAAVTAERY
                     ETSEGRIGELTLERARALRGAWQAAGG"
     gene            14230..15174
                     /gene="cofD"
                     /locus_tag="VFJ72_01720"
     CDS             14230..15174
                     /gene="cofD"
                     /locus_tag="VFJ72_01720"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET7478212.1"
                     /translation="MKVVALAGGVGGAKLAAGLQDALPDGDLTVVVNTADDFDLWGLR
                     ICPDLDTVMYTLSDFANPVTGWGVRKESFNSLGMISLYGEDCWFKLGDRDLATHVLRT
                     NRLREGSSLTDVTAGLSKALGVRGRILPMCDEPVATVLDTQEGPMEFQEYFVRRGQKD
                     EVRGVELRGIEDARITTAAEEAVASADLIALCPSNPVVSIGPVLAVPGIRKTLEAASA
                     PKVAVSPIVGGRALKGPADRMLASLGHESSATGVARIYEGLVDGMVVDLVDEGERAGI
                     EALGMRVLVTQTVMSEPEDRTSLAREVLDFGAKLAERS"
     gene            15182..15778
                     /gene="cofC"
                     /locus_tag="VFJ72_01725"
     CDS             15182..15778
                     /gene="cofC"
                     /locus_tag="VFJ72_01725"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HET7478213.1"
                     /translation="MSVFAVVPVKDLWGTKSRLSPILNPGARAGLTLYMMGRVIRALH
                     EADVSEVGVVSPDPVVLHTAADHGATPVTQESRGLNPALEEGRRWAIDKGASAILVLP
                     ADLPLLDAEDVRAVISHQHEAPSAVISPDAAREGTNALLLKPPDALPFLFGEGSYEAH
                     VRAAVERGVPAQICERPHLSFDLDTADDFQRLKKEGRS"
     gene            15775..>16357
                     /gene="cofE"
                     /locus_tag="VFJ72_01730"
     CDS             15775..>16357
                     /gene="cofE"
                     /locus_tag="VFJ72_01730"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET7478214.1"
                     /translation="MRGIQILPVEGIPEIRPGDDLASILSEAAREDLRPDDVLVVTHK
                     VVSKAEGRLVDLRSVAPSTLAKSYATEHGKDPRQIEVVLRESRRVVRMDRGIIISETR
                     HGFVCANAGVDASNVPGKDTVCLLPLDSDASARKLRGAIAAHLGVEVAVVISDSFGRA
                     WRHGITDIAIGVAGMDPLADYRGQNDPHGYPMEA"
BASE COUNT         2924 a         5167 c         5589 g         2677 t
ORIGIN      
        1 tataaccctc ggccccgcgg gagcgctcgt cgccgacaga gagaccgtca cgcatgtccc
       61 ggctcccgag gtcgaggtcg tggacacgac gggcgcgggg gacgcattcg tcggggcgtt
      121 ggtcgcccgg ctggcacgcg gggacgcgct gccggacgcc gccgcctacg cagttcgggc
      181 cggggccgcg gctgtgacga aggagggggc gcagggggcg ctcccgacgc cggagatggt
      241 ggaagcgctg tgaagagggg cggcatcctg aacgcccggc tctcagcggc gctcgcccgg
      301 ctgggacata cggacctcgt cgtggcctgc gacgccggtc tgacgatacc gttcgtcccc
      361 gaggtccacg atctgctcag ggagggactt ctggatccag ctctcgcgcc tcgcgagaag
      421 ctcaagagga tggtcgccgg cacgaagctc gtcgttcgcg cgggcggggc cacaccctac
      481 tccaacgtca tccttcgttg cggggttccg ttctaattta gttctaattt agttctaaag
      541 gagagccgat atgcattacc ttgaaacgcg gggccttcgg cgatagtcgc ggaaccgttg
      601 ccggcttgat tccccgactc tactctaatc atgtcaacaa ctctcgtgcc ggaacagcat
      661 taagtactaa ttgcacaagc ttgttgatat gtctaagata gatatgaagg gtgggttaag
      721 tagtgatgaa cggaggtaga agataatggc tcaagccagc accgcgaacc gcgcgggaga
      781 atccgcgggt cccagtatga gggtgagggt tcagaggttc ggaagttatc tgagcgggat
      841 ggtcatgccg aacatcggcg cgttcatcgc ctggggtttg atcacggccc tgttcatacc
      901 ggatgggtgg ctaccgaacg agcatctggc gaagctggtg gaacccatga tcctctacct
      961 gctgccgctc ctcatcggct acaccggcgg ccggatggtc tacgacgtgc gaggaggggt
     1021 agtcggcgct atcgccacca tgggcgccat agtcggctcc gacatcccga tgctcctcgg
     1081 cgcgctcatc atgggtccgc tcggcgggta cgtgatcaag aagttcgacg acctcgtaca
     1141 gcacagggtc cgccagggct tcgagatgct ggtgagtacc ttctccgccg gtatcctggg
     1201 cggtatcctc gctatcatcg gggtcctcgg cgccggccct ctcatcacct ccgtgagcaa
     1261 cactctcggg gccggcgtgg acttcatcgt gagcctcggt cttctgccgc tcgtgagcat
     1321 catcgtcgag ccggcgaaga tactgttcct gaacaatgcg atcaaccacg gcatcctcgc
     1381 cccgctcgcc atcgaacagg cgaggcaggc cgggaagtca atcctcttca tggtcgagac
     1441 caaccccgga ccggggttcg gcatcctgct ggcgtactcg gtgttcggac gcggcatctc
     1501 caagcggtcc gcgcccggcg ccgcgatcct ccaggtcctc ggcggcatcc acgagatcta
     1561 cttcccctac gtgctgatga agccgctgct ggtcctcgcc gctatcgctg gcggcatgag
     1621 cggtcttttt accgccacgc tcttcaaggt gggcctcgtc tcgacccccg cgccggggag
     1681 catcttcgcc tacgcagcca tgacgccgcg aggcaacttc gtcggcgtgt tcgcggacgt
     1741 cctcgtagcg accgtcgtgt cgttcctcgt cgcctccgtg ataatccgtc gctcctccga
     1801 catcgagagc gatgatgagg acgagtctag cctcgcgatg gctgcgcagc agatggagac
     1861 gatgaagggc aagaagagca gcgtggccgg taccctgacc agcggcgccg gtacggacga
     1921 gcggaccgga actaacggcc ataccgagac ggctgacaag atagcgcacg cgaagtccat
     1981 cgtgttcgcc tgcgacgcgg ggatgggttc gagcgcgatg ggcgcgtcct tgctcaagaa
     2041 caaggtaaag aaggccggga tggatgacat ctccgtgacc aacgcctcca tcaacaacct
     2101 cacggacgag ccggacatcg tcgtaaccca caaggacctt accgaccgcg cacgggcgaa
     2161 ggctccgagc gcggcgcacg tctccgtgga caacttcctc ggcagcccca agtacgacga
     2221 actggtagag cagatccggc agggcagaga gaacggcggc gcctccaccg gtacagcaga
     2281 cggtacgact gccggtacga gcagcggctc cttcgactat gccggggtcc gcaagatcat
     2341 cttcgcctgc gacgcgggga tgggttctag cgcgatgggc gcgtccctgc tcaggaagaa
     2401 ggccaacgaa gccgggttgt cggagatcga gataaagaac gcctcgatca accagatccc
     2461 cggcgacgcg gacgtgataa tcacgcacag ggatctgacg ggccgggcgg aagagaagat
     2521 gccggaggcc caccacgtct cggtcgacaa cttcctcgaa agcccaaagt acgacgagct
     2581 aatcgaagag atttcgactg cgcgagtcta gggcggggca tggcgcgatg gggctctcgg
     2641 atcgggagat actactgatc gaggtcctgt tgcgccaccc ttacgggctg accgccgaag
     2701 gcattgccga acggctcggc gtgagcgcca ggaccgtcca ccgcgaactt cagcccgcct
     2761 cggaattcct cgattcgcac ggcctgacgc tcttgcgaca ggcgggcagg ggcgtgagcg
     2821 tcgagggcgc gccccaggcg agggagcggg cgctcagcgc cctgcgggag atgggttcgg
     2881 tggggctcac gccggaggag cggcggttgt cgttgctctg caccatgctc gccgccgacc
     2941 agcctatcaa gctgcgggcg ctcgccagcg ggctggacgt cgcggtcggc actgtcagcc
     3001 gcgacttgga cgaggtggag gagtggctca cgggcttccg gctctcgctg ctgcgtaagc
     3061 gcggctacgg ggtcgaggtt gtcggggatg agggtgacag gcgccgggcg atgagccgtt
     3121 tgatcttcga gaacctggac gagtcgaggt tgttcctccg cccggaagac ctcagagaag
     3181 aggactcgac gcccgtccgg gaaccgggcc gcgtggccga tcagttgatg ggcatgatcg
     3241 gcgaggatcg ccttcggagg gtggaggccc tgacggagaa cgtagtcgcc agcctgccgt
     3301 actctatcgc cgacagcgca ttcgtctcga tgagcgtcca cgtcgcgctg atggtcgagc
     3361 gccggttgca gggcggagag atcgagatgg acgaggagat cctgcagagg cttcggaata
     3421 cggaggagta tgagtacgct cggaggctcg ccgcagatat agaagaggac ttccgcgtag
     3481 ccgtccccga agaggaggtc ggctacctga ccatgcacct gcgcggcacg aagctccggg
     3541 aggacgacgc gcttcgagac tacttcgaga cctcggacct ggaggtagcc tcgcaggtga
     3601 aggccctgat ccgctacgtc gaagagcaga ccgggatctc gcttgccgga gatagctctc
     3661 tctacaccgg cctcctcgcc catctggaga gggccatcca tcggctgcga gaacacctgg
     3721 gtatccacaa cccgctcctc tccgaggtaa aggaggacta tccggcgctc tttgacctgg
     3781 tcagtcaggc gatggataag gtgttcgtac aggagaaagt gccggaggaa gaggtcgggt
     3841 tcgtggcgat gcacttcggg gcggcgctcg accgcgggca gggcaacttc ccccggagcg
     3901 tcctcgtaat atgttccagc gggatcggca cctccaagat actcgcctcc cggctgggga
     3961 agtcgttccc ccagatccag acgataagag actcctccct cttcgagctg gattccctga
     4021 acatcgaaga cttcgacctc gtggtatcca cagtgcctct gccgctcccg gacagatcat
     4081 acgtccaggt ccggcccttc ctcaccgacg acgaggtgga gcggatacgg aaccacctcc
     4141 tggagaagag cctggacgcg cgcctggcga accgggcggc attcgagtcc ctggaggtct
     4201 tcggcggggg ccacgcgaag ttccaccaga tggcggaggc tacgcaggtc atagcggagc
     4261 tggtggatga cgcgtacctc gcgcaccacg aggcgggtgg ctccctggaa gtggcggtgc
     4321 ggcggatgtg cgcctcgctc gccgggaagg acctcgtctc ggacccccgc agcctggagg
     4381 agagcctgct ctcgcgtatg gagcttggcg gcatcgggat tccgggtacc gtgctggcgc
     4441 tgttccacgc ccgcgacgcc gccgccgtcc ggccctcctt ctccgtccac gacttcgatg
     4501 agcccctgga gttagaaggg ctcgacggcg cgatgatgcg ggtccggcgg agcctgttga
     4561 tggtggcgcc gctgacgctc tccccggtag ccctggaggc gatcagcgag atctccgctg
     4621 ctatggtcga gcagccggtg gagcgcgagg tcttcgagga cggttcggag gcccggatca
     4681 tcgaagtcct ccagaatatt ttcgcccgtt tcctgcacaa caaactctcg tgaaaactct
     4741 cgtggaagga gcctgtagat gtccgagata ctgaccccgg cgaccataga gctgaaggcg
     4801 aacctggaga gccaggaaga ggcgatccgg cgcgcgggca agctgctggt ggataacggc
     4861 aacgtggatg aacgctacat tgactcgatg ttcgagcggg agaagtccgt ctcgacgttc
     4921 atgggcaacg ccgtggccat cccccacggc acgaacgact ccaagcagtg ggtggagagg
     4981 tccgggctct cggtgatcac cgtccccgag ggcgtggagt acggcgacgg cgatatagcc
     5041 atgctcatca tcgggatcgc cggcaagggc gacgagcacc ttgagatgct ctccaagatc
     5101 gccatcgtgt gctccgagga ggagaacgtc gcaaggatcg tgatgtcgga gtcgaaagag
     5161 gagcttctgt ccatcttcga cgaggtgatg tgaatgctcg gcgttcattt cggggccggc
     5221 aatatcggtc gcggcttcat cggacagcta ctgcacgagt cgggctacga cgtcagcttc
     5281 gttgacgtgc aggcgcagat agtcgaagcc ctgaagaccc agggccgcta cgaggtcatc
     5341 ctggcggcgg agggggagga acgagtcctg gtcgaccgcg ttaccgcgtt ccactccatc
     5401 gacgaggcgg aggaggtcgt ccggcagatc gcaagagcgg acctcgtcac cacggcggtc
     5461 gggccgcccg tgctgcccat cctggctccg gccatagcca atgggctcat ggagcgcgcg
     5521 cggagcggcg gcgggccgct caacgtgatc gcttgcgaga acatgatcgg cggcagcgaa
     5581 tcgctccgaa gctccgtgat ggagcacctc tccggcgagg acgcggaggc cgtggccggg
     5641 atcgccggtt tcccgaacgc ggcggtggac aggatcgtac cggagcagac gaccggcggg
     5701 atcgacgttc tggtcgagcc tttcttcgag tgggtcgtgg acgcctccca ggtgaaaggc
     5761 gaccgcccgg acgtctccgg catcacatac gtcgagagcc tggccccgta catcgagcgg
     5821 aagctcctga ccgtcaacac gggccacgcc gccgtcgcct atctaggcta cgcgcgcggg
     5881 cgggcgacca tctccgacgc cctggaggac gacgccgtct acgaggaggc gtcgggcgcg
     5941 ctggaggaga cgggacgcct gatggtcgaa gagcacggct tcgacgagga ggagcaccgc
     6001 aagtaccggg agaaaactct agtccggttc cgaaacccgc gcatcacgga cgccgtgacg
     6061 agggtcgccc gcacccccat aaggaagctc ggacggaatg agcggctggt ctcgcccgcc
     6121 ctgcggctgc tcgaccgggg atataccccg gtccacctcg ctacggtcat cggggcggtg
     6181 ctgcgctatg acaacgcgga ggacgagcag gcggcggagt tgcaggagaa ggtccgggcg
     6241 gaaggcgagc gcgcggcgct tgcctcctgc gccggtatcg aagaggacca tcccctggtg
     6301 gacctcgtgg cggagcggct gggttcctcc cgcgaattca gccatcagag agggggcggc
     6361 gcgtagtaca ggaagatccg gtggacgctg gagaggtgcg ggctccgcga cccggttaga
     6421 ataggggatg gagagtgtcg cctggtgggg gcggatatta cgccgccggg tttttcgggg
     6481 gcgggaaagg acgataacgg tggcgagcga agtgtatgtg tacgagttct ccgagggctc
     6541 cggggagatg aaggaccttc tgggcggcaa gggagcgggg ctggcggcga tgagcggcct
     6601 cgggctgccg gtgccgccgg gcttcaccat caccaccgag gcatgccggg cctacatgcg
     6661 ggccggcgag cctcccgcgg agctcgcgga gcaggtcgat gcgcatctcg gcgagttgga
     6721 ggagtcaacc ggcaagcggc tgggagaccc ggagaacccc ctgctagtct ccgtgaggag
     6781 tggcgcgccg gtctccatgc cgggcatgat ggacaccgtg ctcaacctgg gcctgagcga
     6841 cgccgcggtt gacgggttcg cgcggaggac cggggacgga cggttcgccc acgactccta
     6901 ccggcgcttc atccagatgt tcggtgacgt ggtcctcaag gttgaccacg agaagttcga
     6961 gaaggccctc gaaacgctaa aagaagagcg cggcgtggag gacgacaccc agctctccga
     7021 ggaggacctg aaagccctga tctcgaccta caagcagata gtggaggagg agacgggaag
     7081 ctctttcccg gaggacccca gggagcaact cgacctcgcc atccgggccg tattcgactc
     7141 ctggaacaac ccgcgggcca acgcctaccg caaggagttc ggcctgccgg acgacctcgg
     7201 gaccgccgtt acggtccagg cgatggtctt cggaaacatg ggcgacgact ctgccacggg
     7261 tgtcgtcttt acccgcgacc cctcgacggg cgagcagggc ctcttcgggg agttcctgct
     7321 caacgcccag ggggaggatg tcgtggccgg catccgcact ccccggccta tctcagagat
     7381 gcaggaggac ctgccgcacg ccttcgagga gcttctggag accatgcagc ggctggagaa
     7441 cgagtaccgc gacatgcagg acatcgagtt caccgtcgag cgggacaggc tcttcatgct
     7501 ccagacccgc agcggcaagc ggaccgcagt agcggcgctc aggatagcct acgagatggc
     7561 ggaggagggc atcatcagcc gcgaggaggc cgtgatgcgg gtcgagccgc gccagttgga
     7621 ccaggtgctc cacccctaca tagaccccga ggcggatctc gacgtgctgg cggtgggact
     7681 tcctgcctca cccggagcgg ccacggggag cgtcgtcttc accgctgatg aggccgagga
     7741 gcggggcgag gcgggggagg cggtcatcct ggtgcggaag gagaccactc ccgacgacgt
     7801 tcacggcatg atgcgggcca agggcacgct cacagcgctc ggcggcatga ccagccacgc
     7861 cgcggtggtc gcgcgcgggc tcgggatacc ggccgtaacc gggtgcaagg cgctcaagat
     7921 cgacgccgcc gcccagacgt tcagcataga cggccagacc tttcgggtgg gagacacgat
     7981 cactatcgag ggctcgaccg gcaaggtggt cgcgggcgag gctcctctgg tggagccgga
     8041 gttgagcgag gacttcgagc gtatcctcgc ctgggccgac gagttccgta ccctcggggt
     8101 gagggcgaac gccgacactc cacaggacgc acggaaggcg cggcagctcg gcgctgaggg
     8161 catagggctg tgccggacgg agcacatgtt catggaagag ggacggctgg agatcatgcg
     8221 cgagatgatc ctctcggaga ccgaggaggc cgagacgcag gcgcttatcc ggctcgaacc
     8281 cctccagcgc ggcgacttcg agggcatctt cgaggcgatg aacggcctgc ccgttaccgt
     8341 gcggctgctc gacccgccgc tacacgagtt cctccccgac tcaagggacc tgtacgaacg
     8401 gctcgccgag ctcggagacg aggagtccga agaggagaag gagatacggc ggcaactgcg
     8461 ggtcgtggag agcctggaag aggcgaaccc gatgctgggc ctgaggggat gccggctggg
     8521 gatcgtccgc cccggactct accggatgca ggcccgcgcc atcgccgagg ccgcgaaagc
     8581 tgtaagagag aaaggcggtt cagtagaggt cgagatcatg gtccccctgg tgggcttcag
     8641 gacagagctt gctgagaccc ggcgagatat cgaggacgtg gtgaatgaag tcctcggaga
     8701 cgcgaacccg gtccgcatcg gcaccatgat cgagctgccc cgcgcctgcg ccgtggccgg
     8761 ggacattgcg aatgaagcgg acttcttctc cttcggcacc aacgacctga cccagacgac
     8821 ctgcggcctc tcccgcgacg atgcggagag cggcttcctg gcacgctacc tccaggaagg
     8881 cattctggag aagaatccct tcgagacgat agatagggac ggcgtcggag agctggtcgc
     8941 gatggcccgc gagaggggcc gggagactaa ccccgacctc aagctgggta tctgcggcga
     9001 gcacggcgga gacccggaga gtatccagtt cttccacgag accggtttgg actacgtctc
     9061 ttgctcacca taccgggtcc cggtggcgcg gctggccgcg gcccagacga ccctgcgcga
     9121 gaggggctcc gtggcgcagg cgagcgccgg gtgatgtagc ggagggaagt acgcgggtcc
     9181 ggtctgtggg agccagacgt gggatcagcg gcggttagcg cccgcgaaga agacggagcc
     9241 gtggacttct agcgggtcgg gatcaaagcc cgcgtgcgcc agcatactcc agatctcgtc
     9301 cgggacgaag aacttgatgc cgctggttga gaggaaacgt tgcagtcgcc gtccgcgtga
     9361 ggtcctggcg cgcaggatac ccatgatggc gatgcggcca cccggttcca gcactcgccg
     9421 ggtctcacgc agggcgcggg ccgggtcccc gaactcgttg agcgagccgc cgcagacagc
     9481 tccggcgaac gagccgtccg cgaacgggag gttctccgcg tccgctcggg cgagagaggc
     9541 gcgggctgct ccagccgccc tgatccggcg cgccgcctcc cggagcatag agggagatat
     9601 gtctacgccg acgacgtcgc cgtttccgtc cagttcacgg gccaggctcc tggtgtagag
     9661 gccggcggaa catcccaggt ccaggtagcg accgccgcgt tccaggttca ccaggcccga
     9721 gatgatctct agctcgcgct cgttcgggaa tggttcgccg gtcaggagcg tgagagaccg
     9781 ggtgcgccag aggggctcgt acccgcgccc ggcgccgggg aggaagttgg tgaggttggc
     9841 gatgttgtat gcgccggacg tgcgcttcag gaggtcgagg tagccgccac gcaccgtgaa
     9901 agtctctccg gtttgttgcg agcggagggt tccgtgcagg atccagccgt cctcctccgt
     9961 accttcgacg agcgtgagac gatcttcgcg cgatggacct acccggatta tgggcaggag
    10021 gtccttccgg aacactaacc gcgcctgcgg tcgcgccggc gatctttcag gcggcccatg
    10081 cgggcgtcga aaccgcgcac cgagtcaacg aacgccccgg actcgcccgg agcggcagcg
    10141 ggcttgagcg tcggatagcc gccctcgtgg aagacgagag ggcccttgcg agtatcgccc
    10201 agccggacct cgacgaccct cccgaggaag aggtcgtggt cgccgccggc gtagactgcc
    10261 tcaagctcgc actctatgac cgccatggcg ccggccgcca ggggcgcacc gaccgtcccg
    10321 tacccgccgc ctaaagagtg gaccgcgtgc agcccgctcg accgctcggg ggaggagaag
    10381 agccgggaga gtccctcctg gtcgtccgcc aggacgctga cggcgaaggt cttctcttcc
    10441 ttgatacgtg ggttgagccg ggcggccttg tggacgctca ccaggaacag cagcgggtcg
    10501 agggagaccg agatcacggc gttcgccgtc atgccctcgg cccgttcgtc ccggccgctg
    10561 gtaacgaccg tcacgcccgt cgggaagtga gacatggcgc ttctcagagc atcggcgttt
    10621 gcgccctgaa cttcccaggg atactcgcgc tctatgtcat ggggcttctc aggcggcatc
    10681 ttcgtctctc tcgacggggt ggaacacggg aaacctacta cgcacggtgc tcttccacaa
    10741 ggggagggac cacacgaagc tcccgcggcc accaccgctc gaccgtctcc aggtccgaac
    10801 cgtaccaccg accgaatggc ggaccatgat agacgccgtc cagcacgacg gacccggcgc
    10861 ccggcagtag cgccgcctcg accggcacga ccccgtctcc ggcgatccgg ccatcctcga
    10921 tgaaacgttc gtaccgcttg cgagcccgcg cggaagagtt tccatccacg gcgtttccgg
    10981 ccacggagag gtagctcagg ccggcgggcg cgtgcagggc gccggggaag aggtcgttga
    11041 cgcgcgctac gggtgcgagt agaccccggt ccgggacatt atgcgggctg ccgagggtga
    11101 tcaggtgcga gacccgccgg tgccccgagt agcgccgtcc gccgtagggc gggtcgccac
    11161 ccaggtagac gcggcaggcg acgccgcccg cggagtgccc gacgagaacc gccttcttcg
    11221 actcgctctc cagcaaggcc cggtcaacgg ccgtcgcgat ctggaatacc agttgcccga
    11281 agccccggat acgtccgagg agccagtcga agggcgtgat attcgccacg tggacctccg
    11341 cgccggagag ctcgtgaagt gtgcgggcca gatctttata tctccacggc caggaagtaa
    11401 cccccccgac gatcactatc ggtccgcgcg ccatgcgcag agtataaacg cgcggatgcc
    11461 gagcgggttg gactctatgt caggctgatg tggatgccgt cgaggacgaa ctggacggcg
    11521 agggcggcga ggaggacacc gagtacgcgg gagagtacgt tcgcgccggt ctcgccgaag
    11581 aggcgcatga tcctgggtgc gatgagcagg gagatcaggg tgagaagcag gaccaccagg
    11641 aggacggcta aaaacagcgc gatctgctcg ggaccgccgc cggtgtagag gagcaccgtc
    11701 gtgatcccgc cgggtccggc taggagcggt atggcgaggg ggaagacgga gatatcctgc
    11761 tggtaggact cctcctcctg ctcgcgcacc gtcgaggagc gcatccccga cgggcgggcg
    11821 aagatcatgt cgagcgacag caggaacagc agtatcccgc ccgcgatcct gaacgccggg
    11881 agcccgatac cgagggcgtg gagcagggtg ttgcccacca gcgcgaagac gaacagtatg
    11941 gccgcgccca ggaacgtgcc ccggatcgcc gactcccgct ttcgttcctc cgagaaccca
    12001 ttggtcagcg ccgcgaagat cggcgccatc cccagaggat ctattactac cagcagcgtc
    12061 acgaaggcat tgaagacgaa ctcgaacacg acgcaactct acaccgcttc cgtatgttcg
    12121 tcagaaacga gagagcaggc tatctagcgg ttggcgaaac gccggacgcg cttcatgtag
    12181 tagctccacc accaccactg gccggggtcc atgtgacagc cggcgcctcc gcccggtccg
    12241 ggacagccgg ggacctcgtt gtgcccgatt atatggttgc ggtttatagg gattccgtaa
    12301 tgccggcaga ggtaggcggt gagcttcgca gatgagcggt acatctcctt cgtgaaccac
    12361 ttggggtcgt ctatgaagcc ctcgtgttct atgccgatgg agtggacgtt gtacggccag
    12421 taaccgcagt ggtaggcgat gttcaggtcg cttaccgact gggcgatctt gccgtcggcg
    12481 gaccgcactg tgtagtgggc cgagactccg gccgaggggt tctggaacca gttcacggcg
    12541 ctcgaccacg acccctgcgt gacgtggatg attatcttgg tgatcctgtc agatttcggg
    12601 cggttggcgc cggtgtagtt gttgggatgg gccgggtacc agacgacggg cggcttggtc
    12661 ggcatcagcg gcctcctatc cgctcgtcga ggctgcgggc cgcgagcgat atcttctcac
    12721 cggacttcgt cttctcggag gcgcctctct tgagggtgtc gaagacctgc tccgcgtaca
    12781 ggtcgccgcc cccgactccg gccacggcct tgtagttctt gccgctggcc ttgccccttc
    12841 ctgcgaccgc gccgaagaag ccccgcaact cctgcggctt ctggcgtccg atggaggcgg
    12901 cgagcagcgc cgccccgccc tttatgttcg aggcgcgatc cgtcttgagc ttatcctcgg
    12961 aaatgccggt gagccttgac gcctcgccga gcgtatcgga ggtcgggttc tgcaccaggg
    13021 ccatgatccc gtacgagccc cagccgtgaa ggtctccttt ctcgtactta ttggagtcgg
    13081 gagccggcat ctcccagcgt gtgttgacgt gtcccatcgc cagcaggacc ccgaccggca
    13141 ccccgtactc tttggcggcg gtgtcgaact ccttcatcaa ccccgacccc ggcttctgcg
    13201 ccgtctgggc ctgtccggta acggcgttgc ccaggagagc aaccccggcg agaccggtcg
    13261 cgcttaactt tagaaacgtg cggcgatcga tcgccactgg catgcctttt agccgcccag
    13321 aacgcattcc ccctccttcg aactggtgca ccttgtttat cgtgttacat taattatcta
    13381 tgtacgcgga ccgggttgca agtcccggcg tcctgaaggt ggatctcaag ggctgtcctc
    13441 taccctcctg ccgcctgcca ggcgccgcgc agagcgcggg cccgttctag cgtaagctcg
    13501 ccgatccggc cttcggaagt ctcgtaacgt tctgctgtga cggcggcgag cgctctcgcg
    13561 acctgtccga cgctcgccgg tatgtcggcg acgctctctg tcctgaacgg gagccccagg
    13621 gcgccgtaga agctctccag gactgccctg atctccgctg tatcccgaac cagtatgagc
    13681 gcctctagac gcgccgcccg gcgcgtaacc ctctgggcta gccccgcgtg cttgacgcct
    13741 agcggcccgc cggagcggac ggagtaggcg ccgggacaga attcgccctc cacctctccg
    13801 ccctcggccg cgacgccaac cctcctcagg gctgccgcca cgaggtccac gccctccgag
    13861 taccgttcgt agagtccgtg ccgcaggtcg tccaccggga aggtcaggga gaaggagagg
    13921 ctgccccggt tcgcagccac cgctccaccg ccggagttgc ggaccaggac cggaaacccg
    13981 gactcgccgg ccaccctggt cgcctcgccg aagcccggaa ggcgggtctc gggccgcgtc
    14041 gcgccgatgt agcgcgaaga ggaccacatc agggcggtcg ggccgcgttc tcccctggag
    14101 gtctcttcga ggaccgcctg ttgcagaccg aagccatcct cgggattctc gaaagggcgg
    14161 gcggtcagga tctccacggt gctaagatag cagtctgaga cttctcatgg atctatggag
    14221 ggacattcga tgaaggtggt tgcgctcgcc ggcggcgtcg gcggggcgaa gctcgccgcc
    14281 ggtctgcagg acgctctccc ggacggagac ctgacagtgg tcgtcaacac cgccgacgac
    14341 ttcgacctct ggggactgag gatctgcccg gacctcgaca ctgttatgta cacgctctcc
    14401 gacttcgcca accctgttac cggatggggc gtcaggaaag agtcgttcaa ctcgctgggc
    14461 atgatctcgc tctacggcga ggattgctgg ttcaagctgg gagaccggga cctggcgacg
    14521 cacgtcctgc ggaccaaccg gctgcgcgag ggctcctcgc tcaccgacgt gacagccggc
    14581 ctctcgaagg cgctcggcgt ccgcggccgc atcctcccta tgtgcgacga gccggtcgcc
    14641 acggtcctcg atactcagga aggaccgatg gagtttcagg agtacttcgt gcggcgcggc
    14701 cagaaggacg aggtgcgcgg cgtagagctg cgcgggatag aggacgcccg gatcaccacc
    14761 gccgccgagg aggccgtggc gtcggcagac ctgatcgcgc tgtgcccgtc caaccccgtg
    14821 gtgagcatcg gaccggtgct cgccgtgccg gggattagga agacgctcga agccgcctcc
    14881 gccccgaagg tcgccgtcag ccccatagtc ggggggcgag cgctcaaggg accggccgat
    14941 agaatgctcg cctcgctcgg acacgagtcc tccgcgacgg gtgttgcccg catctacgag
    15001 gggctggtag acgggatggt cgtggacctc gtggatgagg gagaacgggc cgggatcgag
    15061 gctctgggca tgcgggtact cgtaacgcag accgtgatga gcgagcctga ggaccggacg
    15121 agcctggcgc gagaggtgct ggacttcggc gcgaagctcg cggagcgaag ctaacggagc
    15181 gttgagcgta ttcgccgtag tgcccgtaaa ggacctctgg ggtacgaaga gccggctcag
    15241 cccgatcctg aaccccggcg cccgcgcggg ccttaccctc tacatgatgg gccgcgtcat
    15301 ccgggcgctg catgaggccg atgtcagcga ggtcggcgtg gtcagcccgg accccgtcgt
    15361 cctgcacacc gccgccgacc acggcgcgac gcccgtgacc caggagagcc ggggcctcaa
    15421 cccggctctc gaagaaggac ggcgatgggc gatagataag ggcgcgtcag ccatcctcgt
    15481 cttgccagcc gacctgccgc tgctggacgc ggaggacgtg cgggccgtta tctcgcatca
    15541 gcacgaagcg ccgtcggctg tgatctcccc ggacgccgcg cgcgagggca cgaacgccct
    15601 gcttctgaaa cctccggacg ccctgccgtt cctgttcggc gaggggagct acgaggccca
    15661 cgtccgggcg gcggtcgagc gcggcgtccc ggcgcagatc tgcgaacgtc cgcatctctc
    15721 cttcgacctc gataccgccg acgatttcca gcggcttaag aaagaggggc gatcttgagg
    15781 ggcatccaga tcctgcccgt cgagggaatc ccggagatcc gtcccggcga cgacctggcc
    15841 tctatcctct ccgaagccgc gcgagaggat ctccgcccgg atgatgtgct ggtcgtcacc
    15901 cataaggtcg tcagcaaggc ggagggacgg ctggtggacc ttcgctctgt cgcgccgtcc
    15961 acgctcgcca agagctacgc caccgagcat ggcaaggacc cgcgccagat cgaagtggtc
    16021 ctgcgcgaga gccggcgcgt cgtccgcatg gaccggggca tcatcatctc cgagacgcgc
    16081 cacggattcg tctgcgcgaa cgccggggtg gacgcctcca acgtccccgg aaaagatacc
    16141 gtctgcctcc tgcccctgga ctccgacgcc tccgcccgga agctgcgtgg tgcaatcgcc
    16201 gctcatctcg gagtagaggt ggccgtcgtg atctcggact ccttcggacg cgcgtggcgc
    16261 cacggcatca ccgacatcgc catcggggtg gcggggatgg acccgctcgc cgactaccgg
    16321 ggacaaaacg acccgcacgg ctacccgatg gaggcga
//
LOCUS       DASSMB010000021        14382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_512060, whole genome shotgun sequence.
ACCESSION   DASSMB010000021 DASSMB010000000
VERSION     DASSMB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14382
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512060"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFJ72_01735"
     CDS             <1..237
                     /locus_tag="VFJ72_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7478215.1"
                     /translation="ARHFKPLLKRAGLPDIRLYDLRHTFATLWLESGEHPKILQEILG
                     HSRISVTLDTYSHVIPHMQRDAMGRFGKMFAKPS"
     gene            complement(336..409)
                     /locus_tag="VFJ72_01740"
     tRNA            complement(336..409)
                     /locus_tag="VFJ72_01740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(373..375),aa:Arg,seq:acg)
     gene            530..1216
                     /locus_tag="VFJ72_01745"
     CDS             530..1216
                     /locus_tag="VFJ72_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7478216.1"
                     /translation="MSATVLLVIGDDEERLHLRRALSSEGWRVVPAGSGSQARQMAER
                     QQFEVAVVDNRLPDERGFQVVSAVQKPGVSTVALLRDEDTMDRIVGIELGADYYMRSP
                     VNPRELVARVKQILRKREHAGESAGRLYVGDLEIDPDQVSVRKGEREIMLSPREFKLL
                     YTLASSPGRVFPRAALLRQVWGEDEYIDERTVNVYVQRLRNKLGDSSTDPKLIETVRG
                     FGYRLVKPAS"
     gene            complement(1240..2271)
                     /locus_tag="VFJ72_01750"
     CDS             complement(1240..2271)
                     /locus_tag="VFJ72_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET7478217.1"
                     /translation="MALAAGKGTRLFPLTGEVPKPMAPVVDTPIIEHIFGLLARHGVE
                     EVHVNVHYLADALIEAYGEVSRINGMSVYLDREDELLGTAGGVKRLAEHFDGTFVVVS
                     GDALTDVDLGELVALHKEKEALATIALKRVYDTSEFGVVELDAEGNIMGFQEKPDPKV
                     AISTLANTGIYVLEPRALEYVPEGTFFDFAQDVFPQFLANGEKFVGYQGDFYWSDIGT
                     LQAYRQAQYDVLSGKVGVRVPGEKRGEGLWVGEDAQIHPSANIEGHVLVGRDAVIGRG
                     VTLAGDVTVGTDCWVRPNATIKRSILLPGASVGDGAYLEDCIVGHGYDVRAGETIRGG
                     ALIRRHGTP"
     gene            2349..3248
                     /gene="murB"
                     /locus_tag="VFJ72_01755"
     CDS             2349..3248
                     /gene="murB"
                     /locus_tag="VFJ72_01755"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7478218.1"
                     /translation="MHSDTLHRLFPSAKFDEPLRRYTAWKIGGPADALLEPKSVDELI
                     EAVRKARENNVPVTVLGGGTNVLVRDGGIRGLTIRLAKSLTNVEFDGDRVFADAGVLY
                     PVLANTTAGRGLAGLQFATGIPGTVGGAVYMNAGAYGSETVEVLDWADIFRAGAGEVV
                     RMKNEELGLSYRKSVLHEHPDWVVLRAGYTLSPGDPEDLKAQIKEFRAQRMSGSPSRP
                     SCGSTFKRPPGDFPGRVIEAAGLKGMRVGNIEVSPVHANYLVNLGGGTAEDALALIQK
                     VRETVRERLGIELESEVRVIGEP"
     gene            complement(3262..3894)
                     /locus_tag="VFJ72_01760"
     CDS             complement(3262..3894)
                     /locus_tag="VFJ72_01760"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7478219.1"
                     /translation="MPYFLLTSITMERWDTTESLERVAVFVDGANLYHSIKSYYKGVL
                     DYNQLLAAAVAGRRLLRATFYIVEKQEADENQGAAQGASQGARSFVYNLNKFGYKVRS
                     KPLIVHETVSPEGERTVSHKGDWDIGIIVDMMRLADHADTYVLVSGDGDYVEAIEYLQ
                     SEKGIRVEAISAAQCTSQALLDVCDSHTDLADIPDLFRQKSASPQQPVVG"
     gene            3990..4325
                     /locus_tag="VFJ72_01765"
     CDS             3990..4325
                     /locus_tag="VFJ72_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7478220.1"
                     /translation="MDFEVSIDEHARDYSLISVRGEVDLHTAPKVQYSIERATENGAA
                     AVFVDMSGIAFMDSTALSTFMRLKDALEKQGLPFRLVSPSAAVERIFDVTGFRDYFEV
                     FASREEAAR"
     gene            4406..5932
                     /locus_tag="VFJ72_01770"
     CDS             4406..5932
                     /locus_tag="VFJ72_01770"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7478221.1"
                     /translation="MMTDLKSVEVPEPLERLGEAFRKAGRELYLVGGYVRDRLLDSAH
                     PDVDATTDAHPPEIKKLLRPVAGSIWTVGERFGTIAARVGEYDVEVTTYRSDLYTEGS
                     RHPEVRFGDSLEEDLARRDFTINAIAADALTGAILDPFDGRKDLETGVIRPVGDPLDR
                     MRDDPLRMLRAVRFETTLSTPDKPFAMTPDLERAIRENAHWLESISAERIREEFEKIL
                     LSKNVSRGLRTLVRLGLMRYIVPEFLETVSVEQEAEFHHKDVFEHTMIVTESVETDPI
                     LRKAAFFHDIGKPRTLVYEHRCTYCGAKSTSKSAGEGECERCGGRTLPKKIHFYGHEN
                     VGAAIARRAMKRLAYPKDDTDAVAHLVANHMRPMGYAVGRDPWSDSAVRRFVRDTYLA
                     RGDRELANVDLLLKLARADITGSAPRRRHIAEASWQSLKERVDEVRAEDAIERLTSPL
                     DGNELMEHFGRGPGRWIKPIKDYLEGEVVEGRLAPDDKQKAWQLADAYTREHNVFEEG
                     "
     gene            5937..6812
                     /locus_tag="VFJ72_01775"
     CDS             5937..6812
                     /locus_tag="VFJ72_01775"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7478222.1"
                     /translation="MDNIDPLVSTDWLAERLDDPGVRVVDIRGYVKKTDLGNGRQKAE
                     YLAAREEYDEAHVPGAVYVDWTRDITDPEDPVPAQVAPPERFAALMGSLGIGDNTHVV
                     VYDHAGGQFATRLWWALTYYGHERVSVLDGGWKKWTAEDRPVTVESPNVEPVTFTPKP
                     QPGWRVEAKDVLSASESGGAFILDARDAGQYTGAVARGDGRSGHVPGARHLHADSLLE
                     EGGTFRSDEELAGKLREAGVPEDREAPVVAYCNGGVAATVPLFALHRLGYRNLANYDG
                     SWNEWGSRDDLPAER"
     gene            6909..7493
                     /locus_tag="VFJ72_01780"
     CDS             6909..7493
                     /locus_tag="VFJ72_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET7478223.1"
                     /translation="MSLLMIGGGVLLIGYFFFWHSLSNPSTNSADPGGFNVPKIDDSS
                     QQAAGGPKDKTLKLTIPKMNRVDDAEIPTAPGDDGKALDDHTGIHLEGTGNPWDQEAN
                     VYIAGHRLGYPNTGSFLAFFDLNKLQNGDKVYVTDANGTKYTYEVFKEFSVSPANVSV
                     TKPLPGKNILTLQTCTLPDYSERLIVQAELVDKA"
     gene            complement(7527..8234)
                     /locus_tag="VFJ72_01785"
     CDS             complement(7527..8234)
                     /locus_tag="VFJ72_01785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7478224.1"
                     /translation="MHEKVVMITGASKGLGKALALAFADEGASLVINARSEESIRPVA
                     EEAESRGAEVLAVAADVSKGADVERMVSDAVERFGRIDVLINNAGVLGPRTKLEEHTE
                     EEWRQVIDANLTGPFLVTKAVIPHMPEGGSIINVVSGASAGPRADWGAYAISKFGVEG
                     LTGNLALELKDRGIRVNSVDPGGMRTEMRAAAYPEEDPTTRITPEENTAVFLYLVSDE
                     SRDVTGERLKAQEFGKS"
     gene            8429..9181
                     /locus_tag="VFJ72_01790"
     CDS             8429..9181
                     /locus_tag="VFJ72_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7478225.1"
                     /translation="MYGDNAPPSWARLPGARCRAVEHLTKLEGLQEFEFLAGLEDGFF
                     QALAISAEMLEMGAGTALYREGEPSGAVYFVREGRVKLYRSSGGPKARDQILGVVGDG
                     SVLGLASAIEGRPQSQGATALTDCVLYAVFRDDLIDIMGRFPDGSLRLARTLAARNRD
                     LEDLVGDLVFHSAPQRVACMLLNLATEEGRVTKRGVVFTPSLSRQEMAEATGISREAL
                     SRALSRLAQEGILDLRGKSITILKPAELRSRT"
     gene            9364..9597
                     /locus_tag="VFJ72_01795"
     CDS             9364..9597
                     /locus_tag="VFJ72_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET7478226.1"
                     /translation="MTYVITEPCIGTKDQSCVEVCPVDCIYDAGDHFMINPEECIDCG
                     ACEPECPVEAIYPEDEVPEEMESYITKAADYFE"
     gene            complement(9670..10896)
                     /locus_tag="VFJ72_01800"
     CDS             complement(9670..10896)
                     /locus_tag="VFJ72_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7478227.1"
                     /translation="MDTIRNHDAAAANKRGNQARPERPDPVDFLGLDALLTEEEREVR
                     ERVRTFVREKIHPNIQEWYEKAIFPREIVPEMGELGVLGMHLSGYGCAGKSAVEYGIA
                     CMELEAGDSGLRTFVSVQGSLAMSAIHKFGSEEQKQEWLPRMATGEAIGCFGLTEPTA
                     GSDPASMKTFARKDGSDWVLNGSKRWIGMGSIADVAVVWAGTDDGIRGFLVPTDSPGF
                     TAKNIEPKLSMRASIQCDLTFEDIRLPEEAMLPEAQGLRGPFACLNEARYGIIWGAMG
                     AARDCYECALEYAKQREQFGRPVAGFQLTQQKLVNMMLEIQKGQMVALHIGRMKDAGV
                     LRPEQVSFGKLNNVREAINIAREARTILGGNGVTLEYPMLRHANNLESVRTYEGTDEV
                     HTLILGNAVTGIPAFR"
     gene            complement(10967..11485)
                     /locus_tag="VFJ72_01805"
     CDS             complement(10967..11485)
                     /locus_tag="VFJ72_01805"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7478228.1"
                     /translation="MTVAIPNKIHMTIEEHVIGASVLAPAAPDAFFETVRDVRSFPDW
                     APGVRRVEVLEDPGGPGMLSEWEVSFLGLRKKVLSVLEEAERPEFLRWTYDGPVRGWG
                     ECAIRERGDGTLAEFRTGIVPADRRLRSVMGSSVAREAAHSQLRRALERLGRLAAPDA
                     GRITVGPPMGLL"
     gene            11532..12080
                     /locus_tag="VFJ72_01810"
     CDS             11532..12080
                     /locus_tag="VFJ72_01810"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7478229.1"
                     /translation="MDDGIWRTLGREYLFRSPWCSFRVDDVRLPSGEEIEYGVLESGG
                     LSAVVAVTDGGDVVLVRQWRQPVGGFTLELPSGAVDAGEEPIEAAERELAEETGYRAE
                     GLEHLVSVHTSTGRTTEVCHIFRCRAVLGEGPRPEPTEFIRVVEMPFEEALEEAIGGG
                     INSAATVLGLLGTSLRVRQNQG"
     gene            complement(12071..12490)
                     /locus_tag="VFJ72_01815"
     CDS             complement(12071..12490)
                     /locus_tag="VFJ72_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478230.1"
                     /translation="MMASTTIRVAAVLLVLAAGFTLRITWEQLANPSTPALAQDDQFD
                     CASFGSQESAQTELDRDPSDPSNLDPDGNGIACDNFNFGTDTGNSGGAAQDQYQQDTG
                     NGDLFNAGGPAAGPVPRMPDGDCPSEFPTPRSGACYP"
     regulatory      12677..12794
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12816..13439
                     /locus_tag="VFJ72_01820"
     CDS             12816..13439
                     /locus_tag="VFJ72_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family transcriptional regulator"
                     /protein_id="HET7478231.1"
                     /translation="MRAEDLLERHPELWRAATHHPFLEGLRDGSLPAGAFRMWLVQDY
                     LFVNDLLVFQSRLLARSPRPAQAAIAGGLVALEAELGWFEEMGERRSLALGAPRKPAT
                     AAYRDLLARLDGESYAAGIVALWAIERAYLEAWAGIAPGHPDYREFVEHWTVPEFAGY
                     VAGLEEAADAALEGASGDELQEIEEAFREVSRMERDFWEMALSEGDG"
     gene            13436..14080
                     /locus_tag="VFJ72_01825"
     CDS             13436..14080
                     /locus_tag="VFJ72_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET7478232.1"
                     /translation="MNLSADLWRENRDLAQAALGHPFVRGLGDGTLRLESFQYYVAQD
                     AFFLESFARAYALSLAHSPDSEGLRDFAGLLAGVIEELELHAGYAERWGVSLDEVIPG
                     EATLAYTEFLTATAATRSIGETCAAMTPCMRLYAFLGQSLAEDGYGEENPYAEWIQTY
                     ADPGFEALAARLEGLLDRYSGEEDGARFAYRRAMALEVAFFEAALVSADRPERP"
     gene            14084..>14382
                     /locus_tag="VFJ72_01830"
     CDS             14084..>14382
                     /locus_tag="VFJ72_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7478233.1"
                     /translation="MMYDKPKLAARGVHITKGDVRILDGMDLEVKAGQFVAVIGPSGA
                     GKSTLLGLLAGLEAPDKGVIEKDGVPLDGPGSVAYMPQSDLLLPWRSVVANVSLGL"
BASE COUNT         2481 a         4628 c         4728 g         2545 t
ORIGIN      
        1 gcgaggcact tcaagccgct cctcaagcgc gccggcctcc cggacatccg cctctacgac
       61 ttgcggcaca ccttcgctac gctctggttg gagtccggcg agcaccccaa gatcctgcag
      121 gagatcctcg gccactcccg aatatcagtg acgctcgata cctacagcca cgtaatcccc
      181 cacatgcagc gcgacgcgat gggccgcttc gggaagatgt ttgcgaagcc ctcctgattt
      241 ctgttgtggt atctgttgtg gtacggggcc ggtagagcct agatgaagga agagcccggt
      301 ttgcgtttta cctgcaaacc gaccactttt agtagcgcgc tcggcaggac tcgaacctgc
      361 gaccttctga ttcgtagtca gacgctctat ccggctgagc tacgagcgcg tgaagcctgc
      421 ctatgatacc gctggaggga ggtttgctca aggctggata tgtaggggag gttttgacga
      481 tatactgcag ggggtcgtga ggtctggata gtaaggtagt cgaatagtct tgagcgcaac
      541 ggttctgctg gtcatagggg atgatgagga acggttgcat ctgagacggg cgctgtccag
      601 tgaggggtgg cgggtggtgc cggcggggtc ggggtctcag gcgcggcaga tggcggagag
      661 gcagcagttc gaggtggccg tggtggataa ccggctgccg gatgagcggg ggtttcaggt
      721 cgtctccgcg gtgcagaagc ctggggtctc gacggtggct ctgttgcgcg atgaggatac
      781 gatggaccgt atcgttggta tcgagctagg ggcggattat tatatgcgct ctccggtcaa
      841 tcccagggag ctggtggcga gggtcaagca gatcctccgc aagagagaac acgctgggga
      901 gtctgctggc aggctctatg tcggtgactt ggagatcgac ccggaccaag tctctgttcg
      961 gaagggggag cgcgagatca tgctctctcc ccgcgagttt aagctgctct acactctggc
     1021 gagtagcccc ggacgggtct ttccccgtgc cgctctgctc cgacaggtct ggggcgaaga
     1081 cgagtacatc gacgagcgga ccgtcaacgt ctatgtccag cgcctgcgca acaagctcgg
     1141 cgacagctcc acggatccca agctaataga gaccgtccgg ggtttcggtt accggctcgt
     1201 gaagcccgcg agttaacacc aaagtcagta ctgcactggt cagggggttc cgtgacgccg
     1261 gatcagggcg ccgccccgga tcgtctctcc ggcccgcacg tcgtagccgt ggcccacgat
     1321 gcagtcttcc aggtaggcgc cgtcgccgac gctcgcgccg ggcagcagga tgctccgctt
     1381 gatggtggcg ttcgggcgca cccagcagtc cgtcccgacc gtcacgtccc cggccagggt
     1441 tactccgcgt ccgatcacgg cgtccctccc taccagcacg tgaccctcga tgttggcgga
     1501 aggatgtatc tgggcgtcct ccccgaccca cagaccctct ccgcgcttct cacccgggac
     1561 cctgacaccg acctttcccg acagaacatc gtactgcgcc tgacggtacg cctgtagcgt
     1621 cccgatatcc gaccagtaga agtctccctg atacccgacg aacttctccc cgttcgcgag
     1681 aaactgcggg aagacatcct gcgcgaagtc gaagaaagtc ccctccggga cgtactccag
     1741 cgctcgcggc tccagtacgt agatgccggt gttcgccagg gtgcttatgg caaccttcgg
     1801 gtcgggcttc tcctgaaatc ccattatgtt gccctccgcg tcaagctcca ccaccccgaa
     1861 ctccgaggtg tcgtagaccc ttttgagggc gatggtggcc agagcctcct tctccttgtg
     1921 aagggctacc agctccccga ggtccacgtc cgtcagggcg tctccggaga ccaccacgaa
     1981 ggtgccgtcg aagtgctcgg cgagacgctt tacgcctccc gccgtgccga gcaactcgtc
     2041 ttcccggtcg aggtatacgc tcatgccgtt gatacgggag acctcgccgt acgcttcgat
     2101 cagcgcgtcg gccaggtagt ggacgttgac gtggacctcc tctacgccgt gccgggcgag
     2161 aaggccgaag atgtgctcga tgatcggggt gtccaccaca ggggccatcg gcttcgggac
     2221 ctcgcccgtc agcgggaaaa ggcgggtccc cttgcccgcc gcgagcgcca tagctttcaa
     2281 ctcatacctg ccattctcta tctgtgccgg gcctgcaaaa cgctcacccg taccgtatat
     2341 tccctctcgt gcattcggac actctacatc gcctcttccc gtcggccaag ttcgacgagc
     2401 ccctgaggcg ttacaccgcc tggaagatcg ggggtcccgc cgacgcgctg ctcgaaccga
     2461 agagcgtcga cgagctgatc gaagccgtgc gcaaggcccg cgaaaacaac gtcccggtta
     2521 ccgtcctcgg cggcgggacg aacgtcctgg tgcgcgacgg cggcatccgg ggactcacca
     2581 tccggctcgc caagtccctg acgaacgtcg agttcgacgg ggaccgcgtc ttcgccgacg
     2641 cgggcgtcct ttacccggtc ctcgccaaca ccaccgccgg gcgcggactt gccgggttgc
     2701 agttcgccac cggcatcccc ggcaccgtcg gcggcgcggt ctacatgaac gccggagcct
     2761 atggctccga gacggtggag gtcctcgact gggccgacat cttccgggcg ggcgccggag
     2821 aggtcgtcag gatgaagaac gaggagcttg gcctctcgta ccgcaagagc gtcctgcacg
     2881 agcaccccga ctgggtcgtc ctgcgcgccg ggtacacgct ctcgcccggc gacccggagg
     2941 atctgaaggc ccagataaag gagttccgcg cccagcgcat gagcggctct ccgagcaggc
     3001 cgagttgcgg ctcgaccttc aagcgtccgc caggagactt ccccggccgc gtcattgagg
     3061 cggcgggcct gaaagggatg cgcgtcggaa acatagaggt ctctcccgtc cacgccaact
     3121 acctcgtcaa cctcggaggc ggcaccgccg aagacgcgct ggccctgatc cagaaggtcc
     3181 gggagacggt gcgggagagg ttggggatag agcttgagtc ggaagtgcgg gtgatcgggg
     3241 agccgtagct cctggagcgc cctacccgac cacgggttgc tgggggctcg cggacttctg
     3301 gcggaagagg tccgggatgt cggcgaggtc cgtgtgagag tcgcagacgt cgaggagggc
     3361 ctgggaggtg cactgggcgg cgcttatggc ctcgacccgg atacccttct cgctctgcag
     3421 gtactctatg gcctcgacgt agtcgccgtc gccggagacg aggacgtaag tatccgcgtg
     3481 gtcggcgagt cgcatcatgt ccacgataat gcctatgtcc cagtcgccct tgtgcgagac
     3541 ggtgcgctcc ccctcggggc tcacggtctc gtggacgatg aggggcttgg agcggacctt
     3601 gtagccgaac ttgttgaggt tgtagacgaa ggaccgcgcg ccctgcgagg cgccctgggc
     3661 cgcgccctgg ttctcgtcgg cctcctgctt ctccacgatg tagaaggtgg cgcgcaggag
     3721 cctgcggccg gcgacggcgg ccgcgagtag ctggttatag tccagcacgc ccttgtagta
     3781 gctcttgatc gagtgataca ggttcgcccc gtccacgaag accgccacgc gctccaggct
     3841 ctctgtcgtg tcccaccgtt ccatagttat ggatgttagc agaaaataag gcatgttccg
     3901 ccaggaaagc ggtttaccgg atgcggttat tgagttaata tccctaggcc ggcacggctt
     3961 cgagagcgca gagaggggtg gcacccggca tggacttcga ggtaagcata gacgagcacg
     4021 ccagggatta ctccttgatc tcggtgcgcg gagaagtgga cctgcacacc gccccgaagg
     4081 tccagtactc tatagagcgc gcgactgaga acggcgctgc cgcggtattc gtagatatga
     4141 gcggtatcgc gttcatggac tcgaccgccc tctccacttt catgcgcctc aaggacgccc
     4201 ttgagaaaca ggggctaccc ttccggctcg tctctccatc cgcagccgtc gagcgcatct
     4261 tcgacgttac tggcttccgc gactacttcg aggtcttcgc ctcccgcgaa gaagccgccc
     4321 gctagagaac acgcggctcc tggtggatcc tccagggcga gagccttcta gacgcgcgtt
     4381 tcattcgtac tagaatctga tcctgatgat gacggacctg aagagcgtcg aggtgccgga
     4441 gcctctggag cgcctgggag aggcgttccg gaaggccggg cgcgagctgt acctcgtcgg
     4501 cggatatgtt cgcgacaggc tgctcgactc cgcgcacccg gacgtggacg ccaccacgga
     4561 cgcccatccg ccggagatta agaagctact gcggcccgtg gccggaagca tctggaccgt
     4621 gggagagagg ttcgggacca tcgcagccag ggtcggagag tacgacgtcg aggtgacgac
     4681 ctaccgcagc gacctctaca cggaggggag ccgccacccg gaggtccgct tcggggacag
     4741 tctggaggag gacctggcgc ggcgggactt caccataaac gccattgcgg cggatgctct
     4801 gaccggcgct atcctggacc cgttcgacgg ccggaaggat ctggagaccg gcgtgatccg
     4861 tccggttgga gacccgctgg accggatgcg cgacgacccg ttgaggatgc tgcgagccgt
     4921 ccgcttcgag acgacgcttt cgacacccga caagccgttc gcgatgacgc cggacctcga
     4981 acgggcgatc cgggagaacg cccactggct ggagagcatc agcgccgagc gcatccggga
     5041 ggagttcgag aagatcctgc tctcgaagaa cgtgtctcgc ggcctgcgga cgctcgtgcg
     5101 cctggggctc atgcgataca tcgtgccgga gttccttgag acggtctccg tggagcagga
     5161 ggcggagttc caccacaagg acgtcttcga gcacaccatg atcgtcacgg agagcgtcga
     5221 gacggacccg atcctccgca aggccgcctt tttccacgac atcggcaagc cgcgcaccct
     5281 cgtctacgag caccgctgca cctactgcgg cgcgaagagc accagcaaga gcgccggaga
     5341 gggcgagtgc gagcgctgcg gcggcaggac gctgccgaag aagatccact tctacggcca
     5401 cgagaacgtc ggagcggcaa tcgcccgccg ggccatgaag cgtctcgcct acccgaagga
     5461 cgacacggac gccgtcgccc acctcgtcgc caaccacatg cgcccgatgg gctacgccgt
     5521 gggccgcgac ccgtggagcg actccgccgt ccggcgcttc gtccgggata catacctggc
     5581 gcgcggcgac cgggaactgg cgaacgtgga cctcctgctc aagctcgctc gcgccgacat
     5641 caccggcagc gcccccagga ggcgccacat cgccgaggcg tcctggcaga gcctgaagga
     5701 gcgcgtcgac gaagtccggg cagaggacgc catcgagagg ctcaccagcc cgctcgacgg
     5761 gaacgagttg atggagcact tcggacgggg tccgggacgc tggatcaaac cgataaaaga
     5821 ctaccttgaa ggcgaggtcg tcgagggccg cctcgccccc gacgacaaac agaaggcctg
     5881 gcaactcgcc gacgcctaca cccgcgaaca caacgtattc gaggagggat agagacgtgg
     5941 ataacataga tcctctcgta agcacggact ggctcgcgga gcgcctggac gaccccggcg
     6001 tccgagtcgt ggacatccgg ggctacgtga agaagacgga tctgggaaac ggccgccaga
     6061 aggccgagta cctcgccgcc cgcgaggagt acgacgaggc ccacgtaccc ggcgccgtct
     6121 acgtggactg gacccgcgac atcaccgacc ccgaggaccc ggtcccggcc caggtcgctc
     6181 cgccggagcg cttcgccgcg ctgatgggct cgctggggat cggcgacaat actcacgttg
     6241 tcgtctacga ccatgccggc ggccagttcg ccacgcgcct gtggtgggct ctcacctact
     6301 acggtcacga gcgcgtctcc gtcctcgacg gcggctggaa gaaatggacc gccgaagacc
     6361 ggcccgtcac ggtggagagc ccaaacgtcg agcccgtcac cttcaccccg aagccgcagc
     6421 cgggctggcg cgtcgaggcc aaggacgtcc tatccgcgag cgagagcggc ggggctttca
     6481 tactggatgc ccgagatgcg ggacagtaca ccggcgcggt agcgaggggc gacggccgat
     6541 ccgggcacgt ccccggcgcg aggcacctgc atgccgacag cctcctcgaa gaaggcggca
     6601 cgttccgttc ggacgaggag ctagccggga agctgcgcga ggccggggtg ccggaggacc
     6661 gcgaggcgcc cgttgtcgcc tactgcaacg gcggtgtcgc ggcgaccgtg ccgctcttcg
     6721 cgttgcaccg gctcggttac aggaacctcg ccaactacga cggctcgtgg aacgagtggg
     6781 gctcaaggga tgacctgccc gccgagcgct aatacggtga cggtgaagat caggtgttac
     6841 actctccggg tcttatgaag aagtacagcg tcaaaaagtc gcgcgccagg ggcgcggtag
     6901 cggggctgat gagcctcctt atgatcggcg gaggggtact cctgatcggg tacttcttct
     6961 tctggcatag cctctcgaac ccttcgacca acagcgccga ccccggcggg ttcaacgtcc
     7021 cgaagataga cgactccagc cagcaggccg ccggcggtcc caaggacaag actctcaagc
     7081 tcaccatccc caagatgaac cgggtggacg acgccgagat tcctactgcc cccggcgacg
     7141 acggcaaggc tctggacgac catacaggca tacacctcga aggtaccggg aacccgtggg
     7201 accaggaggc taatgtctac atcgccggac accgtctcgg ctacccgaac accggcagct
     7261 tcctggcctt cttcgacctg aacaagctgc agaacgggga caaagtctac gtcaccgacg
     7321 ccaacgggac gaagtacacc tacgaggtgt tcaaggagtt ctccgtcagc cccgcgaacg
     7381 tctccgtgac caagccgctg ccggggaaga acatcctcac cctccagacc tgtaccctcc
     7441 ccgactactc cgagcggctc atcgtgcagg ccgagctggt agacaaggcg taagccgaac
     7501 cgcttctcgc ttgcatcacc gaagacctag cttttcccga actcctgcgc cttgaggcgc
     7561 tctcccgtta cgtctctcga ctcgtcggag accaggtaca ggaagacggc ggtgttctct
     7621 tcgggggtga tgcgggtcgt ggggtcttcc tccgggtagg cggcggcgcg catctctgta
     7681 cgcatgccgc cggggtcaac ggagtttacg cggatgccgc ggtcctttag ttcgagcgcc
     7741 aggttcccgg tgaggccctc gacgccgaac ttgctgatag cgtacgctcc ccagtccgcg
     7801 cggggaccgg cgctagcccc gctcacgaca tttattatgc tcccgccctc cggcatatgg
     7861 gggatgacgg ccttcgtgac gaggaaaggc cccgttagat tggcgtcgat tacctgccgc
     7921 cactcttctt cggtgtgctc ttcgagcttc gtgcgcgggc ccaggactcc ggcgttgttt
     7981 ataaggacgt ctatgcggcc gaagcgctcc acggcgtcgg agaccatgcg ctccacgtcc
     8041 gcgcccttcg agacgtccgc cgcgacggcc aggacttcgg cgccgcggga ctcggcctct
     8101 tcggcgacgg gacggatact ctcctcgctg cgggcgttga tcaccagcga tgctccctcg
     8161 tcggcgaacg cgagcgcgag cgccttccca agacccttgc tcgcgccggt gatcataact
     8221 accttttcgt gcatgatgtc tcctctttgg ccgccggttt ggccttttga tcccgatagt
     8281 ctccgggttt ttcactctcc tgctgtccgc cccagtttac gatgttcgcg cgagcgcttc
     8341 ctctggttga ctcgcttacc tgcaaatctt agccacttag cgtataaagg gtgtatggaa
     8401 tatccgagat cccgacgcgc ccgacaccgt gtacggtgat aacgcgccgc cgtcctgggc
     8461 gcggctaccg ggcgcgcgtt gccgggccgt cgagcacctg acgaagctgg aagggttgca
     8521 ggagttcgag ttcctggcgg ggcttgagga cgggttcttc caggctttag cgatctccgc
     8581 ggagatgctg gagatggggg cgggcacggc gctctaccgc gagggcgagc cgtccggcgc
     8641 ggtgtacttc gtgcgcgagg gacgggtgaa actgtaccgc tcttccggcg ggccgaaggc
     8701 gcgggaccag attctgggcg tcgtcggcga cgggtcggtg ctggggctgg cctcggccat
     8761 cgaggggcgt ccgcagagcc agggcgcgac cgccctgaca gattgcgtcc tctacgccgt
     8821 cttccgggac gacctgatcg acatcatggg acgcttcccg gatggctcgc tgcgcctcgc
     8881 ccggactctc gccgcccgca accgggacct cgaagatctc gtcggcgacc tggtcttcca
     8941 cagcgcgccc cagcgcgtcg cctgcatgct cctgaacctc gccaccgagg agggccgcgt
     9001 caccaaacgg ggcgtagtct tcacgccctc gctctcccgc caggagatgg cggaggcgac
     9061 cggcatctcc cgcgaggccc tctcccgcgc cctcagccgc ctcgcgcaag agggcattct
     9121 ggacctccgg ggcaagtcca tcaccatcct caagcccgcc gaactgcggt cccggaccta
     9181 gcgccaggct ggttccggcc aacggtattt gagaacgtct agcaataaga acggtttttg
     9241 atctggatca catcgcgcga tgatcgcggt catgggtcgt tatgatgcgc gtcatcggcg
     9301 ggtgttgggg gtgagggcgg ctggtataaa gcggctagac gaagtgttga gaggaggaac
     9361 cggatgacat acgtgatcac cgagccgtgt atcggcacga aggaccagag ttgcgtggag
     9421 gtctgcccgg tcgactgcat ctacgatgcg ggcgaccact tcatgatcaa cccggaagag
     9481 tgcatcgact gcggcgcctg cgagccggag tgcccggtcg aggcgatcta cccggaggac
     9541 gaggtacccg aggagatgga gagctacatc accaaggccg ccgactactt cgagtaaccg
     9601 cgccgcgtag cttctagcag aatgagggcc gctccgtact ggagcggccc catttttatg
     9661 cttgaacttc tacctgaagg ccgggatgcc ggttacggcg tttccgagga tgagggtgtg
     9721 gacctcgtcg gtgccctcgt atgtcctgac gctctccagg ttgttggcgt ggcggagcat
     9781 cgggtactcc agcgtcacgc cgttgccccc gaggatggtg cgcgcctcgc gggctatgtt
     9841 tatcgcctcg cgcacgttgt tcagcttgcc gaacgagact tgctcgggtc gtaggactcc
     9901 cgcgtccttc atgcgcccga tgtgcagcgc gaccatctgg cccttctgga tctccagcat
     9961 catgttcacg agcttctgct gcgttagctg gaagcctgcc accggacggc cgaactgctc
    10021 gcgctgtttg gcatactcga gggcgcattc gtagcagtcg cgggcggcgc ccatcgcgcc
    10081 ccagatgatg ccgtaccggg cctcgttgag gcaggcgaac ggtcctctga ggccctgcgc
    10141 ctccggcagc atcgcctcct cggggagccg tatgtcctcg aaggtcaggt cgcactggat
    10201 gctggcgcgc atcgagagct tcggctcgat gttcttcgcc gtgaagccgg gcgaatcggt
    10261 ggggaccagg aagccccgga taccgtcatc ggtccccgcc cagacgaccg cgacgtcggc
    10321 gatggacccc atcccgatcc agcgcttcga gccgttgagg acccagtcgg agccgtcctt
    10381 gcgcgcgaag gtcttcatgc tcgccggatc gctcccggcc gtcggctcgg tgaggccgaa
    10441 gcagcctatg gcctcgcccg tcgccatccg cggcagccac tcctgtttct gctcctcgct
    10501 cccgaacttg tggatcgcgc tcatcgcgag cgagccctgc acggagacga aggtcctcag
    10561 acccgagtcc ccggcttcca gttccatgca ggcgatgccg tactctaccg cgctctttcc
    10621 ggcgcagccg tagccggaga ggtgcatgcc gagaaccccg agctcgccca tctccgggac
    10681 gatctcgcgg gggaagatcg ccttctcgta ccactcctgg atgttcggat ggatcttctc
    10741 cctgacaaag gtgcgtaccc gctcacgtac ctcgcgctcc tcctcggtga gcagcgcatc
    10801 gagccccagg aagtctaccg ggtccgggcg ctccggcctg gcctggttcc ctctcttgtt
    10861 tgcagcagcc gcgtcatgat tccgtatcgt atccatacgg cgagtctacc ccggatgcaa
    10921 cttctcgcgc aatgaacgcg cccacacgcc gttgctacaa cgctcgctag agcagaccca
    10981 tcggcggccc gacagttatc cgcccggcgt ccggggcggc gagacgtccc agcctctcca
    11041 gcgcgcgccg cagttgtgaa tgagccgctt ctctggcgac agaagagccc atcacggacc
    11101 gcagacgccg atccgccggg acgatgcccg tcctgaactc cgcgagcgtt ccgtctcctc
    11161 gctcccttat cgcgcactcg ccccagcccc gcaccggacc gtcgtaggtc catcgtagga
    11221 actccggacg ctccgcctcc tccagcacgc tcaggacctt cttgcgcaga cccagaaacg
    11281 agacttccca ctccgagagc atccccggcc cgcccgggtc ctccaacacc tcgacccgcc
    11341 gcactcccgg cgcccagtca gggaagctcc gcacgtcccg caccgtctcg aagaaggcat
    11401 caggagccgc aggcgcgagg acgctcgccc cgatcacatg ctcctcgata gtcatatgta
    11461 tcttgttcgg tattgctact gtcatgtagc aagattatac atcctgcgcc cggatgtcta
    11521 tactccgcgg tatggatgat gggatttggc ggacgttggg ccgggagtat ctgtttcgga
    11581 gcccgtggtg ctcctttcgg gtggacgatg tgcgtctgcc gagtggggag gagatcgagt
    11641 acggggtcct tgagagcggc gggttatccg cggtagtcgc cgtcacggac gggggagacg
    11701 tggtgctggt gcgccagtgg cggcagccgg tcgggggttt cacgctggag ttgccgagtg
    11761 gggcggttga cgccggggag gagccaatcg aggcggcgga gcgggagctg gccgaggaga
    11821 cgggctaccg ggcggagggg ttggagcacc tcgtctctgt ccacaccagc acggggcgca
    11881 cgaccgaggt ctgccacatc ttccgctgcc gggcggtcct tggcgagggg ccgcggccgg
    11941 agccgacgga gttcatccgg gtagtggaga tgccgttcga ggaggctctt gaggaagcta
    12001 ttggcggcgg gataaacagc gccgcgaccg tgctcgggct cctggggacg agcctaagag
    12061 tcaggcagaa tcaggggtag catgccccgc ttcgtggtgt ggggaactcg gacggacagt
    12121 cgccgtccgg catccggggg acagggcccg ccgcaggtcc gccggcgttg aacaggtcgc
    12181 cgttgccggt gtcttgctga tactggtcct gagccgctcc gccgctgttg ccggtatcgg
    12241 tcccgaagtt gaagttatcg caggctatgc cgttaccgtc ggggtcgagg ttgctgggat
    12301 cgctcgggtc ccgatccagc tccgtctgag ccgattcttg agagccgaag ctcgcgcaat
    12361 cgaactggtc gtcctgcgcc agagccgggg tggacgggtt tgcgagctgc tcccaggtga
    12421 tgcggagggt gaagcccgcg gccagaacca ggagtaccgc cgccacccgt atagtcgtgc
    12481 tcgccatcat ggaagcattc tacgtcagtc ggtaccactg cgcaccggtc tacctgaccg
    12541 gtccacctga tgcatagccg gagagggttc atctcgcaac atattggtgc tggctcgcga
    12601 cggacgccga ggctcctctt ctatggaggg gcgaatgggt tgtctgccgg ttggcggaag
    12661 agtagagtat ggaggtaacg aagggccggg gagccgggag gctgagaggg catgggacgg
    12721 tggaatgagt ccggtgccga cccgctgaac ctgatccggg taatgccggc ggagggaaat
    12781 atgagtgact catccgcgat ctttcacggc cgctcatgag ggccgaggac cttctggagc
    12841 gccatccaga actctggcgc gcggcgaccc accatccatt tctcgaaggt cttcgcgacg
    12901 gctccctgcc cgccggggcg ttccggatgt ggctcgtcca ggactatctg ttcgtcaacg
    12961 acctgctggt cttccagtct cgcctgctcg cgcgctcccc gcgtccggcg caggccgcta
    13021 tcgccggggg gctggtcgcg ctggaggcgg agctcggctg gttcgaggag atgggggagc
    13081 ggcggagtct cgcgctcggc gcaccccgca agcccgccac cgccgcttac cgggacctgt
    13141 tagccaggct cgacggagag agttacgctg cgggcatcgt ggccctgtgg gcgatagagc
    13201 gagcctatct ggaggcctgg gcgggcatcg ctccgggaca tccggactac agggagttcg
    13261 tcgaacactg gacggtcccg gagttcgcgg gctacgtggc cgggctcgaa gaggccgcag
    13321 acgccgctct ggaaggagcc tccggagacg agctgcaaga gatcgaagaa gccttccgcg
    13381 aggtctcgcg catggagcgg gacttctggg agatggcgtt atcggagggt gacgggtgaa
    13441 tctgtcagcc gatctctgga gagagaaccg cgacctcgcg caggcggcgc ttgggcaccc
    13501 cttcgtgcgg ggtctcggcg acgggaccct gcggctcgaa agcttccagt actacgtggc
    13561 gcaggacgcc ttcttcctgg agagcttcgc ccgggcctac gcgctgtcgc tcgctcacag
    13621 cccggattcc gaaggattgc gagacttcgc cgggttgctg gcgggcgtga tcgaggagct
    13681 ggagctgcac gcgggctacg cggagcggtg gggagtgagt ctggatgaag tcatccccgg
    13741 cgaggcgacc ctggcctata cggagttcct gaccgcgacc gccgcgacga gaagcatcgg
    13801 cgaaacctgc gccgcgatga cgccctgcat gcggctctac gcgtttctcg ggcaatcgct
    13861 ggcggaggac gggtacggcg aggagaaccc ttacgcggag tggatccaga cctacgccga
    13921 ccccggcttc gaggcgctcg ccgcccggct cgaagggttg ctcgaccgct actccggaga
    13981 agaggacggg gcgcggttcg cctaccgccg ggcgatggcc ctcgaagtcg cgttcttcga
    14041 ggcggcgctc gtctccgccg atagaccgga gcgcccgtaa cgaatgatgt acgacaaacc
    14101 caagctcgcc gcccgtgggg tccacatcac caagggagat gtgcgcatcc tcgacggcat
    14161 ggacctggag gtgaaagccg ggcagttcgt cgccgtgatc ggaccctccg gcgcgggcaa
    14221 gtcaaccctc ctcggcctgc tcgccgggct ggaagcgccg gataagggcg tcatagagaa
    14281 ggacggagtt ccgctcgacg gccccggctc cgtcgcctac atgccgcaga gcgacctgct
    14341 cctcccctgg cggagcgtcg tggcaaacgt ctctctcggc ct
//
LOCUS       DASSMB010000022         2106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1526184, whole genome shotgun sequence.
ACCESSION   DASSMB010000022 DASSMB010000000
VERSION     DASSMB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526184"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VFJ72_01835"
     CDS             complement(<1..1246)
                     /locus_tag="VFJ72_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7478234.1"
                     /translation="MLPGYDEHLKLIETARGERPAELFVRGGTIANVYSGELREGNVA
                     VTGGRIAYVGEGERAVGPDTRVVDASGMIVAPGYIEAHFHPWVLYNPVSLVEGILPLG
                     TTTVIADNLFFFMQMGMERFAAMANDLRDLPLLYLWMARLTSQAKFPGEEAMFALDMV
                     EPLLRRDDVIGTAEVTRWPALAAGDPHLISGIRAAKALGKVVDGHTGGASEARLQPVA
                     AAGIDADHEAITRQEVLNRLRLGIWTMLRNSSLRPDLPELLRVVTEDGVSTQRLIMTT
                     DGPAPEFVADNGLVDGMLRAAVEHGVPPMQALQMVTINPATLFRIDDQVGGIGIGRRA
                     DLLLLPDLTSFRPETVITRGRVVVEDREICVPLPRLDWEKYRSRPRFDSGLDLADPAL
                     YPVRASGTEARVPAMHLKSAVIS"
     gene            complement(1250..>2106)
                     /locus_tag="VFJ72_01840"
     CDS             complement(1250..>2106)
                     /locus_tag="VFJ72_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7478235.1"
                     /translation="TVIDPGFDYPPAREVWESKTAEIGLDLERDVARIIVTHLHPDHI
                     GLVRWLEERSGAPVWMLEGEIENARHVWDPDRGSEDFVRFLVRNGMDRETAEATAGTT
                     RLGIRLPERLNALHRDEEIELGSSAARVIHTPGHSDFHFVLHDEERRLLFAGDQLLLK
                     ITPNIGRWTYTAPHPLERYLDSIKGLRGMDVDLVFPGHGPLFHDLDGRIDELVLHHEE
                     RLSVMHAAFDGEPATPFEIARRVFADDLTHHQLRFALAETLAHLECLADEGRAEYLDG
                     DVVRYRAV"
BASE COUNT          328 a          712 c          708 g          358 t
ORIGIN      
        1 cggagatcac ggcggacttc aggtgcattg ccggcaccct cgcctccgtc ccggacgctc
       61 tgaccgggta gagtgcggga tcggcgagat ccaggccgct gtcaaagcgc gggcggctcc
      121 ggtatttctc ccagtcgaga cgcgggagcg gaacgcaaat ctctctgtcc tcgacaacca
      181 cgcgcccgcg cgtgatgacc gtctcgggac ggaacgaggt gaggtcgggc aggagcagga
      241 ggtccgcgcg gcggcctatg cctatgccgc ctacctgatc gtctatcctg aacagcgtgg
      301 ccggatttat cgtcaccatc tggagcgcct gcatcggcgg cacgccgtgc tcgacagcgg
      361 cgcggagcat accgtccacg agcccgttgt cggcaacgaa ttctggcgcg gggccgtcgg
      421 tcgtcatgat caggcgctgc gtgctcacgc cgtcttcggt gacgacgcgc agcaactccg
      481 gcaggtcggg gcgcagggag ctgttgcgga gcatcgtcca gataccgagc cgcagccggt
      541 tgaggacctc ctgtctggtt atggcctcgt ggtcggcgtc tatgcccgcg gcggcgacgg
      601 gttgcaggcg agcctccgag gccccgccgg tgtggccgtc cacgaccttt ccgagggcct
      661 tcgccgcgcg gatgccggag atcaggtgcg ggtctcccgc cgccaaagcg ggccagcgcg
      721 ttacctctgc cgtcccgatc acgtcgtcgc gccgcaggag aggctccacc atatccagcg
      781 cgaacatcgc ctcctcgccg gggaacttcg cctgcgaggt gaggcgcgcc atccacaggt
      841 acaggagcgg caagtcccgc aggtcgttgg ccatcgccgc gaaccgctcc atccccatct
      901 gcatgaaaaa gaacaggttg tcggcgatta cggtggtcgt accgagcggc aggatgccct
      961 cgacgaggct gaccgggttg tagaggaccc acgggtggaa gtgggcctct atgtagcctg
     1021 gagcgacgat catgccgctc gcgtccacca cccgcgtgtc ggggccgacg gcccgctctc
     1081 cctccccgac gtaagcgatg cgcccccccg tgacggcgac attgccctcg cgcagctcgc
     1141 cggagtagac gttcgctatc gtgccgccgc gcacgaatag ctcggcggga cgttcgccgc
     1201 gcgccgtctc gataagcttc aggtgttcgt cgtagcctgg caacacggtc tagacagccc
     1261 ggtagcgaac cacgtcgccg tccagatact cggcacgccc ctcatcggcg aggcactcca
     1321 ggtgggcgag cgtctcggcg agggcgaagc ggagctggtg gtgggtcagg tcgtcggcga
     1381 agacgcgccg cgctatctcg aagggggtcg ccggctcgcc gtcaaaggcc gcgtgcatga
     1441 cagagagacg ctcctcgtgg tggaggacga gctcgtcgat tcgtccgtcg aggtcgtgga
     1501 agagcggccc atgccccggg aagacgaggt ctacgtccat acctcgcagg ccctttatgg
     1561 agtcgaggta gcgttccagg ggatgcgggg ccgtgtaggt ccagcggccg atgttcggcg
     1621 tgatcttgag cagcaactgg tccccggcga acaggagcct gcgttcctcg tcgtgcagga
     1681 cgaagtggaa gtccgaatgt cccggcgtgt ggatgacgcg cgccgcgctg ctccccagct
     1741 cgatctcctc gtcccgatgc agggcgttca gtcgctccgg cagccggatg ccgagcctcg
     1801 tggtcccggc cgttgcttcc gccgtctccc ggtccatacc gttgcggacc aggaacctca
     1861 cgaagtcttc cgaacctctg tccgggtccc agacgtggcg ggcgttctcg atctcccctt
     1921 ccagcatcca gaccggcgcc ccgctccgct cctccagcca gcggacgagc ccgatgtggt
     1981 ccgggtgcag gtgcgtcacg atgatccgcg ctacgtcgcg ctccaggtcc agaccaatct
     2041 ccgccgtttt ggactcccag acctcgcggg ccggcgggta atcgaagccg ggatctatga
     2101 ccgtcc
//
LOCUS       DASSMB010000023        13132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_638986, whole genome shotgun sequence.
ACCESSION   DASSMB010000023 DASSMB010000000
VERSION     DASSMB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13132
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638986"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..552
                     /locus_tag="VFJ72_01845"
     CDS             298..552
                     /locus_tag="VFJ72_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478236.1"
                     /translation="MLRAMKKGAREMYNDWFYLEQSRVQREQERVRRMAGRAKCAQLR
                     AARLSLRARLARRLFALAVAAERQETWKVVWERLEAKGRL"
     gene            585..1370
                     /locus_tag="VFJ72_01850"
     CDS             585..1370
                     /locus_tag="VFJ72_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7478237.1"
                     /translation="MKALELKNVEKSFKKRRGEVVRALDSVSLSVGKGEVVGILGPNG
                     SGKSTLVRVISTLVVPDAGTVEVFGVDAVRHPKQVQRSMNRVSVEASFFKKLSAMENL
                     LYGAKLYGVTTGEARPRIKEILTHIGFAHKRALEPMEHLSRGMQQKIALARALLTSPV
                     LMLLDEPTTGLDPRSKRDVQAFIHRIREAHDSSILLCTHDMGEAEELCDRVGIMVDGK
                     ILALEEPEELKRRYATNGKSPTLEETFMAATGYSVEEASAEEE"
     gene            1380..2222
                     /locus_tag="VFJ72_01855"
     CDS             1380..2222
                     /locus_tag="VFJ72_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7478238.1"
                     /translation="MQPLHKPRWMVEVIAVWGYVQRNYFLTKRYAMWELVWLTFTTVN
                     AMSITFIGAGVGLVGGGQGIDTERLMTYLLVGALIWSYLSMLFDVLSETVSWERWEGT
                     IEYTFMSPTSRVTHLLGMGVYAVIYGIMRTGIILGVVSLMFELDLSNANYWGALLVLA
                     ACSISLVGFGVVAAVMPLLSPEKGQQVTYIISALLLLVSGVYYPVSVLPGWMQAIAKF
                     SPVTYALDGSRAALLRGAGAAELWDSIWPLLVMGAVFVPLGLWIFHLGEGYAKRTGKL
                     KRSG"
     gene            complement(2234..2794)
                     /locus_tag="VFJ72_01860"
     CDS             complement(2234..2794)
                     /locus_tag="VFJ72_01860"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET7478239.1"
                     /translation="MAEQFTPHGSLTVEEYLKMEETATVKHEYVAGTIHAHAGASSRH
                     NLIAGNIFAGLWNAARGGSCRVYGSDMRLRAAEDTFYYPDVMVVCGDNEAGDGALYQD
                     APCLVVEVTSPSTEQIDRREKVAAYRKIPTLKAYLVVAQDRRWIERHWLADDGEWRQG
                     GMADEGSIPVPCPETRLSLDDVYEGL"
     gene            complement(2908..3093)
                     /locus_tag="VFJ72_01865"
     CDS             complement(2908..3093)
                     /locus_tag="VFJ72_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HET7478240.1"
                     /translation="MQANPLRYAWDYLKEISGENAYDRYLTVHAATHPDKPAISRGEF
                     YRHRQDEKYNNPGSRCP"
     gene            complement(3100..5211)
                     /locus_tag="VFJ72_01870"
     CDS             complement(3100..5211)
                     /locus_tag="VFJ72_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET7478241.1"
                     /translation="MGSLGRYAIWIVIAIIGALCWGMLAFSRGETVNAAWLLFAALAS
                     YAIAYRFYARFIQNRVLETDDSRATPAERLNNGRDFEPMDRRVLFGHHFAAIAGAGPL
                     VGPVLAAQMGFLPGTIWIIVGVILAGAVQDMTTLFFSMRRNGKTLGQMAREEIGPVGG
                     AAALIAVLSIMIILLAVLALVVVNALAASPWGSFSLAMTLPIAILMGIYLRFIRPGRV
                     LEVSLIGVALLMLAIIGGGWISESSAYADAWTFTPNQITFALIAYGFIASVLPVWVLL
                     APRDYLSTFMKIGTIALLAVGIALTFPSMQMPRATDFAFNGQGPVFAGSLFPFVFITI
                     ACGALSGFHALVASGTTPKLIQKESQVRMIGYGAMLMESFVAVMAIIAATTLDPGVYF
                     AMNAPAGVLGDTAQSAAQAVQGFGFSVDPNTLTQTAKAVGEESIIGRTGGAPTLAVGI
                     SAIFAGVTGFLGAGLQAFWYHFAIMFEALFILTTVDAGTRVGRFMLQDMIGNVWRPFA
                     RTSWLPGNIVASAIIVGAWGYFLYVGVNDPLGGINQLFPLFGIANQLLAAVALTVGTT
                     ILIKMGKLRYAWVTALPLVWDASVTLTASFIKIFSPDPKLGFFAQRGAAQEALAKGEL
                     FGTAQSAQDVQQIIVNSTVDGILSILFATMIIIVIADAVRVWFGLIRGGKEPVMSEAP
                     WEESRLDSEGQPLEREPVGAR"
     gene            complement(5326..6048)
                     /locus_tag="VFJ72_01875"
     CDS             complement(5326..6048)
                     /locus_tag="VFJ72_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7478242.1"
                     /translation="MRINPPDAYWKKDVTLFFSKKVALVIKFIYPLALIVPVAASDIP
                     AQYAAAVIGIVSIMAGTFGAGESLTIDLNDGILARVALTPLSPRRIVFEVLAVNAVLD
                     FLQLLPALLVAYALHPAPLVWVLAATFGVFMTLVTANCIGIFIANFTTSPADVLLYAT
                     AILFPLIYLSGFFRNQHPTGILATLREFVPFAYMNDALKTVFGLATQTTPFEVFLYPT
                     LICVVLTIAVLFTSPRLLSVRL"
     gene            complement(6038..6979)
                     /locus_tag="VFJ72_01880"
     CDS             complement(6038..6979)
                     /locus_tag="VFJ72_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7478243.1"
                     /translation="MQPTLEAKDITRRYRSSGRGVVGASLSVAPGEVFGLMGPNGSGK
                     STLLRVLSTAIEPDSGELGICGADALREKRRVRARMGLMVDRPTHYDDLTGWANAYFF
                     ARAYGVTEEKADGVLAELFDYFDLSEYRDDRVKTYSYGMGKKLALIEAMAHGPALLLL
                     DEPSLGLDYTSQLAYQRKIRDLADAGVAVIIASNQVDEVESLCDRVAFLHRGRIVAGG
                     TPEELISTVEGVRRIVATLRNPVEYGPIEQTSGVQRVVPEGRDLVVHCDERPGVVADV
                     VAGIVRAGGDIVSLSVERPNLEDVFVKLTGEALKDED"
     gene            7116..7604
                     /locus_tag="VFJ72_01885"
     CDS             7116..7604
                     /locus_tag="VFJ72_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478244.1"
                     /translation="MARSALPMLFLLAALLLAGCSGAPKQEARSSAEDTKQRTVAEPT
                     GEQTTTSVENTGSENAGGSGGPEYIVIGESEREEGGVRTAQVIVTAKKTSKRELRNIV
                     EDLKDKYRNTDAASVEIMDGPAGFGKAGSAVIMNTDEGARHFGYPDGVPNSRGYVLKT
                     AD"
     gene            complement(7639..8352)
                     /locus_tag="VFJ72_01890"
     CDS             complement(7639..8352)
                     /locus_tag="VFJ72_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET7478245.1"
                     /translation="MRRGIFVMAALAAACALLASAGAMRPAEAQTQVLNQPVVLGEYA
                     TNYLWDSDPGRRINLAVASQAIDGTILAPGAVFSFNDSVAGQSFAPAKVIIEGKVDYA
                     EGGGLCQVSSTLYMAANYAGLKILERSPHSAELPYIQPGFDATVWLGLKDMKFKNTTG
                     SYVIIREWMSSDGNVYAQIYGQPTGKQVAMGSYKSDSGTDKNGDGFTVWKTYKTVTRN
                     GKVLFDGLLHTDTYHELKH"
     gene            8570..9445
                     /locus_tag="VFJ72_01895"
     CDS             8570..9445
                     /locus_tag="VFJ72_01895"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7478246.1"
                     /translation="MPGKTTVAVLGTGIMGAAMARNLLKAGMEVRVWNRSREKAEPLA
                     DDGARVADTPAEATDGADFLLTMLAEADAVSESVADGALANLSESGVWLQMSTVGVEG
                     NERLARLAEESGTSYVDAPVLGTKQPAEQGQLIVLASGPEEVREKCEPVFDAVGSKTV
                     WLGEAGAGSSLKLVVNNWIVGLLGVLAETIALAKATGVDPAKFLETIEGGPLGLPYAQ
                     MKGKMMISEDFPTSFSAKLARKDVGLVLDAAEAQSLQLTIAEAVAARFDEAIEAGYGE
                     ADMSAIYESAGVGRA"
     gene            complement(9450..10013)
                     /locus_tag="VFJ72_01900"
     CDS             complement(9450..10013)
                     /locus_tag="VFJ72_01900"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7478247.1"
                     /translation="MKNSSAAHFVVPGLIGAVAGLLAGNRLIKTYDWRTEWSIPAPTP
                     AVYEAMTSREALRQWWPSMELADEQDEDELRVGSTVSFQVHQAPEVARLAPPFHIHCV
                     YTDVEPERRLREVVAGDLTGVLETLFTESPDGGGTQVTFNWYVRVTNPALNLLGYVAE
                     RMYRHSHDSVMSEGEEGLRRYCARASR"
     gene            complement(10027..11439)
                     /locus_tag="VFJ72_01905"
     CDS             complement(10027..11439)
                     /locus_tag="VFJ72_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478248.1"
                     /translation="MRPGEPTSETAARIHESYDDLARYPLLEALIERRSRRFGGGMTL
                     NGGPLAYQSRRPPEPLSLEEEAALAFAGCGITGYALAELPYESGEAPDAGGGNIMTHF
                     VGRTVPSGDAMHYITLFVINDEGVWMLKRPQDHPRAEIASLSRAAREHKLVELYEKSR
                     IRISDKRLDVPREPPFVPPFNQWSANLTGTTYFLPIAECTAIYINILLSAFGEEFAYF
                     VVDERNRFKPPGIARFARSRGGYLHDDPREGRFATVGFIETWLYEFGAIEQGGMLQNL
                     ALMTQALGLGGFPHFAAHPFTWFQALGFRMQEVPFSRTIGAGAVTKRLLKALNKDLPV
                     PTAVGLERGGEVQIKPFCPPYYRTMKEAVLAFVDYKYAQGEGTFRDGGAATAWQDGAR
                     IQAGIPPYSEEAIAATISYCEYVYNRYGRFPANSGPFRTVLAYQAHHLDPDFYDRFYR
                     PEALAETQRRHPAHPPGNSQ"
     gene            complement(11436..11885)
                     /locus_tag="VFJ72_01910"
     CDS             complement(11436..11885)
                     /locus_tag="VFJ72_01910"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7478249.1"
                     /translation="MARVYNTVQIQMPIEQVFDYATTPGNWPRWHPSSLGVSGATDHS
                     LKPGEQVTERFLVAGRRGTVVWTVREREAPRRWVIESRTESGGSGKITYALTPRDGGT
                     AFEREFVYSMPNAMLALLDRVVFRRRIEAESAEALRRLKEVLERRAA"
     gene            complement(11908..12537)
                     /locus_tag="VFJ72_01915"
     CDS             complement(11908..12537)
                     /locus_tag="VFJ72_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478250.1"
                     /translation="MTTRTVESGAVPSRRSIPFERFAGTCAVLTGVTGFLYAVAFVVL
                     QNTLLSGLFLMLGGLLTTAVLVAVYERLRRTEATFALWALLLGMAGALGSAAHGGYDL
                     ANAVNPPPSIPDLPNPIDPRGLLTFGVAGIALLIVAWLIVRGGQFPERLGYLGYASAV
                     LLVALYLGRLIFLDPASPLIVVPALLSGFLINPLFYLWLGLALLRGRDS"
     gene            complement(12566..13039)
                     /locus_tag="VFJ72_01920"
     CDS             complement(12566..13039)
                     /locus_tag="VFJ72_01920"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7478251.1"
                     /translation="MPTLDPNGEIQIPGSGFGPAGEEISQFVRELVEAWNSHDAGRVQ
                     DFYAPEYEGTDVGQAMPYHGPQGASRHMSGYLRAFPDLSFTQDEIIVQGARVALFWTA
                     HGTHEGKLMNIPPTGKKIAVHGASQLVVQNGQVIRGLYIWDVAGLLRTIGLLPEL"
BASE COUNT         2420 a         4113 c         4311 g         2288 t
ORIGIN      
        1 cgagaaattt caagcaatac tgttgacaag ctctatagga gggagtagag tgcccgcgct
       61 gttgttgtcg cgaaaggacc gagagtagat ggcatttcgc ctcgcgaaca taggaactcc
      121 ggtgttcggg cgagaggtgc gtcccgaggt ccgcgcggtc gcggtgagaa cgtgagctag
      181 acagctcgac aaaagttttc tctaagaggc ccccggactt acggaccggg ggcctaattt
      241 tttgcggaat agccgcatca aaggctcccg actccgaact cgaagtaaac cgtcccggtg
      301 ctacgcgcga tgaagaaggg agcaagagag atgtacaacg actggtttta tctggagcag
      361 agccgcgttc agcgggagca ggagcgcgtc cggcgtatgg ccggacgcgc gaagtgcgcg
      421 caactgaggg cagccaggct ctcgctgcgg gccaggcttg cgcgccgcct cttcgctctc
      481 gcggtcgccg ccgagcggca ggagacgtgg aaggtcgtct gggagcggct ggaggcgaag
      541 gggcgcctgt gatggcgctc cttgagacag agaggaactc gaaggtgaag gcgttggaac
      601 tgaagaacgt agagaagagc ttcaagaagc ggcggggaga ggtggtgcgc gcgctcgact
      661 cggtgtcgct ctcggtaggg aagggggagg tcgtcggcat cctcggcccg aacgggagcg
      721 gcaagagcac gctcgtgcgc gtcatctcca cgctcgtcgt cccggacgcc ggcacggtcg
      781 aggtcttcgg agtagacgct gtgcggcacc ccaagcaggt gcagcggagc atgaaccggg
      841 tcagtgtcga ggccagcttc ttcaagaagc tctcggcgat ggagaacctt ctctacgggg
      901 cgaagctcta cggcgtcacg accggcgaag cgcgcccgcg cataaaggag atcctgaccc
      961 acatcggctt cgctcacaag cgagctctgg agccgatgga gcacctgagc cgcgggatgc
     1021 agcagaagat cgcgctcgcg cgggcgctgc tcacgagccc cgtcctgatg cttctggacg
     1081 agccgactac gggcctggac ccgcggagca agagggacgt gcaggcgttc attcaccgga
     1141 tacgggaggc gcacgactcc tcgatcctgc tctgcaccca cgacatgggt gaggcggagg
     1201 aactgtgcga ccgggtcggc atcatggtgg atggaaagat actggcgctg gaagaacccg
     1261 aggaactgaa gcgccgctat gcgacgaacg gtaaatcgcc gacgttggag gagacgttca
     1321 tggccgcgac cggctactcc gtcgaagagg ccagcgcgga agaagaatag gagaacagta
     1381 tgcagccctt gcacaagccc cggtggatgg tggaggtgat cgccgtctgg ggctacgtac
     1441 aacgtaacta cttcctgacc aaacgctacg cgatgtggga gctagtctgg ctcacgttca
     1501 cgaccgtaaa cgccatgtcc atcaccttca tcggggcggg cgtcggcctc gtcggcgggg
     1561 gccaggggat agacaccgag cgactgatga cctacctgct cgtcggggcg cttatctgga
     1621 gctatctctc gatgctcttc gacgtcctct cggagacggt tagctgggag cggtgggaag
     1681 ggaccatcga gtacaccttc atgagcccca ccagtcgggt tacgcatctt ctcgggatgg
     1741 gcgtctacgc ggtcatctac ggcatcatgc ggacgggcat catcctcggc gtcgtctcgc
     1801 tgatgttcga gttagacctc tccaacgcca actactgggg cgcgctgctc gtgctggcgg
     1861 cgtgcagcat ctcgctcgtc ggcttcgggg tcgtcgccgc cgtgatgccg ctcctctccc
     1921 cggagaaagg ccagcaggtc acgtacataa tctccgcgct cctgctgctc gtgtccggcg
     1981 tctactatcc cgtgagcgtg ctccccggct ggatgcaggc catagcgaag ttctcgccgg
     2041 taacctacgc tctcgatggc tcccgcgcgg cgcttctccg aggagccggt gcagcggagt
     2101 tatgggacag catctggccg ctgctcgtga tgggggccgt attcgtgccg ctgggactct
     2161 ggatcttcca cctcggcgag ggctacgcca aacggaccgg caagctcaag cggagcggat
     2221 gaggacggcg gccttacaga ccctcgtaga cgtcatcgag ggagagacgg gtctcggggc
     2281 aggggacggg gatcgagcct tcatcggcca taccgccctg cctccattcc ccgtcgtcag
     2341 cgagccagtg ccgctcgatc cagcggcggt cctgggcgac tacaaggtac gccttgagcg
     2401 tgggaatctt cctgtacgca gctaccttct cgcgccggtc tatctgctcg gtgctcgggg
     2461 aggttacctc gactaccagg cacggagcgt cctggtagag tgctccgtca ccggcttcgt
     2521 tgtcaccgca taccaccatc acgtcgggat aatagaaggt gtcttcggcg gctcgtagcc
     2581 tcatgtcgct gccgtagacg cggcaagagc cgccacgagc cgcgttccag agaccggcaa
     2641 aaatgtttcc cgctatgagg ttgtggcgac tgctggctcc ggcgtgcgcg tgaatagtac
     2701 cggccacgta ctcgtgctta acggtggcgg tctcctccat cttcaggtac tcctcgacgg
     2761 tcagggagcc gtggggtgtg aattgctctg ccatgcaacg agtatagcgt aaccagtaag
     2821 ggtttcatcg cagaaaagag gggagggaga gagcccgccc ttcgttctat ctatgcctca
     2881 cggaccgacc ggcccgccgg gacgtcatca cggacatcgg ctgccggggt tgttgtactt
     2941 ctcgtcctgg cggtggcggt agaactcgcc gcggcttatg gccggtttgt ccggatgagt
     3001 cgcggcgtgg actgtcaggt agcggtcgta ggcgttctcg ccggagatct ccttcaggta
     3061 atcccaggcg tagcggagag gattcgcctg cattgttccc tatctcgctc ccacgggctc
     3121 gcgctccaga ggctgtccct cggagtccag gcgggactct tcccacgggg cctcgctcat
     3181 cacgggctcc ttgccgcctc ggatgaggcc gaaccacacc cgaacggcgt ccgcgatgac
     3241 gataatgatc atggtcgcga acaggatgga gagtatcccg tccaccgtgg agttgacgat
     3301 gatctgctgc acgtcctgcg cgctctgcgc cgtgccgaag agctctccct tcgcgagcgc
     3361 ctcctgggcg gctcctctct gggcgaagaa cccgagcttc gggtcggggg agaagatctt
     3421 gatgaagctc gccgtgagcg ttaccgaggc gtcccacacc agcggcagcg ccgtcaccca
     3481 agcgtagcgg agcttgccca tctttatcag gatggtggta ccgacggtaa gggctaccgc
     3541 cgccagaagc tggttggcga tgccgaagag cgggaagagc tggttgatgc cgccgagcgg
     3601 gtcgttcacg ccgacgtaca ggaagtagcc ccacgccccg acgatgatgg cgctggcgac
     3661 gatgttgccc ggcagccagg aggtgcgggc gaacggccgc cacacgttgc cgatcatgtc
     3721 ctggagcatg aaccgcccga ccctggtccc cgcgtcaacg gtggtcagga tgaacagcgc
     3781 ctcgaacatg atggcgaagt ggtaccagaa cgcctgcaag cccgcgccca ggaagcccgt
     3841 aaccccggcg aagatcgccg agatgcccac cgcgagcgtc ggagcgccgc ccgtgcggcc
     3901 gatgatcgac tcctcaccga cagccttagc cgtctgcgtg agcgtgttgg ggtccacgga
     3961 gaacccgaag ccctgcacgg cctgcgccgc cgactgggcc gtgtcgccga ggacgcccgc
     4021 cggggcgttc atggcgaagt agacgccggg atcaagcgtg gtagcggcga tgatcgccat
     4081 cacagcgacg aaggactcca tcagcatcgc cccgtaaccg atcatgcgta cctgcgattc
     4141 tttctggatg agcttcggcg tcgtcccgga ggcgaccagc gcatggaagc cggagagcgc
     4201 cccgcacgct atggttatga agacgaaggg gaagagggag cctgcgaaga ccggaccctg
     4261 gccgttgaag gcgaagtccg tggcccgcgg catctgcatg gacgggaagg tgagggctat
     4321 gccgacggcg aggagggcta tcgtgccgat cttcatgaag gtcgagaggt agtcgcgcgg
     4381 ggcgaggagc acccacaccg gcaggaccga ggcgatgaag ccgtaggcga tgagggcgaa
     4441 ggtgatctgg ttcggggtga acgtccacgc atcggcgtag gcggaggact ccgagatcca
     4501 accgcccccg atgatcgcga gcatcaacag cgcgaccccg atgagcgaga cctcaagcac
     4561 ccgccccggc cggatgaacc gcaggtatat gcccatgagg atcgcgatcg ggagcgtcat
     4621 cgccagcgag aacgatcccc acggcgacgc cgccagcgcg tttacgacca cgagcgccag
     4681 caccgcgagc aggatgatca tgatggagag aaccgcgatc agggcggcgg cgccgccgac
     4741 cggaccgatc tcctcccgcg ccatctgccc gagcgtcttg ccgttacgcc gcatggagaa
     4801 gaagagcgtc gtcatgtcct gcaccgcgcc cgccaggatg accccgacga tgatccagat
     4861 ggtccctggc aggaagccca tctgcgccgc gagcaccggc ccgacgagcg gaccagcccc
     4921 cgcgatggcc gcgaagtggt gcccgaagag cacccgccgg tccatcggct cgaagtcgcg
     4981 gccgttgttc agtcgctcgg cgggtgtggc ccgcgagtcg tccgtctcca acactctatt
     5041 ctggatgaag cgcgcgtaga aacggtatgc gatggcgtag gaggccagcg ccgcgaagag
     5101 taaccatgca gcattcaccg tctccccgcg cgagaaggcc aacatccccc agcacagcgc
     5161 gccgatgatg gcgatgacga tccagatggc atacctgccg agactgccca tagattcctc
     5221 ctgcctctcc cagattcttc cgcgaaacct gcgtgcatgc taacacaacg gtttgccgta
     5281 ttgaacagga ttaagggacg aaagccgccg gtccgccgga gatcgttaga gcctgacgga
     5341 gagcaggcgc ggtgacgtaa agaggaccgc gatggtcagt acgacgcaga tgagcgtcgg
     5401 atacaggaag acctcgaacg gggtggtctg ggtcgccagg ccgaagacgg ttttcagggc
     5461 atcgttcatg taggcgaagg ggacgaactc tcgcagcgtc gccagtatgc cggtcgggtg
     5521 ctggttgcgg aagaagccgg acaggtagat cagggggaag agtatagcgg tcgcgtagag
     5581 cagcacgtcc gccggcgagg tggtgaagtt ggcgataaag atcccgatgc agttcgccgt
     5641 cacgagcgtc atgaataccc cgaacgtcgc cgcgagcacc cacacgagcg gcgcgggatg
     5701 cagcgcgtac gcgacgagca gggccgggag tagctgcagg aagtccagca ccgcgttcac
     5761 cgccagcacc tcgaagacga tgcggcgcgg cgagagaggg gtgagggcca cgcgggccag
     5821 gatgccgtcg ttcaggtcta tcgtcaggct ctcgcccgcc ccgaacgtcc cggccatgat
     5881 cgagacgatc ccgataacgg ccgccgcgta ctgcgccggg atgtcgctcg cggcgacggg
     5941 cacgatgagc gcgagcgggt agatgaactt tatgaccagc gcgaccttct tggagaagaa
     6001 cagcgttacg tccttcttcc agtaggcgtc cggcgggtta atcctcatcc ttcagcgcct
     6061 ctccggtcag cttgacgaag acgtcttcca ggttcgggcg ctcgactgac aggctcacga
     6121 tgtccccgcc agcgcgcacg atgcccgcca ccacgtccgc cacgaccccc ggccgctcat
     6181 cgcaatggac gacgaggtct cgaccctccg gcacgacgcg ctgcacgccg gacgtctgct
     6241 cgatggggcc gtactctacc gggttgcgca gcgtcgccac gatccgacgg acgccctcca
     6301 cggtggagat caactcctcc ggcgtccctc cggcgacgat ccttccccgg tgcaggaacg
     6361 ccacccggtc gcagagagac tcgacctcgt ctacctggtt gctggcgata atgacggcca
     6421 cgcccgcgtc cgcgaggtcc cggatcttgc gctggtacgc gagctgcgag gtgtagtcga
     6481 gaccgagcga aggctcgtcc agaagaagca atgccggacc atgagccatc gcctcgatga
     6541 gcgcgagctt ctttcccatc ccgtaggagt aggtcttgac cctgtcgtcc cggtactcgg
     6601 agaggtcgaa atagtcgaac agctcggcga ggacgccgtc agctttctcc tccgtgacgc
     6661 cgtaggcgcg ggcgaagaag taggcgttcg cccagcccgt gaggtcgtcg tagtgggtcg
     6721 ggcggtccac catcaggccc atccgggccc ggaccctcct cttctcgcgg agcgcgtccg
     6781 cgccgcagat cccaagctct cccgagtctg gctcgatagc cgtcgagagg acgcgcagga
     6841 gcgtgctctt gccgctcccg ttcggtccca ttaggccgaa gacctcaccc ggcgcaaccg
     6901 agaggctcgc gcccacgacg ccgcggccgc tgctcctata ccggcgggtg atgtccttcg
     6961 cttccagggt gggttgcata cgagcacatt ctagccgggc cggatgggtc ccgttgttag
     7021 ccgtattacc cctgtcggaa cctgcggagg atgcgttagc tctcggattc gcataacata
     7081 acgccaggat taaccgcgcc aggataggag ggagtgtggc ccggagcgcg ttaccgatgt
     7141 tgttcctgct cgcagccctg ctcctcgccg gttgctcggg agccccgaag caggaagccc
     7201 gctcgtcggc cgaggatacg aaacagcgca ccgtcgccga acctaccggc gagcagacca
     7261 cgacgtccgt ggaaaacacc ggttcagaga acgccggtgg cagtggtggg ccggagtaca
     7321 tcgtgatcgg cgagtccgag cgggaggagg gcggcgtcag gacggcgcag gtgatcgtca
     7381 ccgccaagaa gacctccaaa cgagagttgc gcaatatcgt ggaggacctc aaggacaagt
     7441 accggaacac cgacgcggcc tcggtggaga tcatggacgg cccggccggc ttcggcaagg
     7501 cgggctcggc cgttatcatg aacaccgacg agggagctcg ccacttcggc taccccgacg
     7561 gtgtgcccaa cagccgcggc tacgtgctga agaccgcgga ttagagggga cgccctcagc
     7621 gctacgctgc gctccggcct agtgcttaag ctcgtggtag gtgtccgtgt gcagcaggcc
     7681 gtcaaatagt accttgccgt tccgcgtgac ggtcttgtac gttttccaga cggtgaagcc
     7741 gtcgccattc ttgtcggtgc cggagtcgga tttgtaggag cccatagcga cctgcttgcc
     7801 ggtgggctgg ccgtagatct gtgcgtagac gttcccgtcg ctgctcatcc actcgcggat
     7861 gatgacgtag gagccggtgg tgttcttgaa cttcatgtcc ttcagcccga gccagacagt
     7921 agcatcgaaa cccggctgga tgtacggtag ctcggccgag tgcggggagc gctccaggat
     7981 cttgagcccc gcgtagttgg ccgccatgta tagcgtcgag gacacctggc acagcccgcc
     8041 gccctcagcg tagtcaacct tgccctctat gatgaccttc gcaggagcga agctctgtcc
     8101 cgccaccgag tcattgaaag agaagaccgc gcccggcgcc agtatcgtgc cgtctatggc
     8161 ctgtgacgcg acagccaggt tgatccgccg gcccggatcg ctgtcccaca ggtagttggt
     8221 ggcgtactct cccagcacga ccggctggtt cagcacctgc gtctgcgcct cggcgggtct
     8281 catggctccc gcggacgcca gcagggcgca cgccgccgca agagcggcca tcacgaatat
     8341 tcctcttctc aacaggaccc cctcctgatt catatagagc cgaaactcct cggaggctct
     8401 ccagacaccg tcggagagac gccgggatcg gcttccgcac cacatattac cccgcggcgc
     8461 attcgttaca caagaggtag tgcatgagaa atgtggcccc ggtacgcatc cagcatgcta
     8521 accttcctct ctatgacggt tttgaaggcg agatccagga ggaagagtca tgcccggtaa
     8581 gacgaccgta gccgttctcg gcacgggcat catgggcgcc gcgatggcgc gcaacctgct
     8641 aaaggcgggg atggaagttc gcgtctggaa ccgctcccgc gagaaggccg aaccgctcgc
     8701 ggacgacggc gcgagggtgg cggatactcc ggcagaggca accgacggcg cggacttcct
     8761 cctcaccatg ctcgcggagg cggacgccgt ctcggagtcc gtggcggatg gcgcgctggc
     8821 gaacctctcc gaaagcggcg tctggctcca gatgagcacg gtcggggtgg agggcaacga
     8881 acggctcgcg cggctggcgg aggagagcgg cacctcctac gtggacgcgc cggtcctggg
     8941 gacgaagcag cccgccgagc agggccagct aatagttctc gcctccgggc cggaggaggt
     9001 ccgcgagaag tgcgagccgg tcttcgacgc cgtcgggagc aagacggtgt ggctcgggga
     9061 ggcgggcgcg ggcagcagtc tcaagctggt ggtgaacaac tggatcgtgg gtctactcgg
     9121 cgtgctggcc gagactattg ccctcgcaaa agccactggc gtggacccgg cgaagttcct
     9181 ggagaccatc gagggtggtc cgctcggtct gccctacgcg cagatgaagg ggaagatgat
     9241 gatctcggag gacttcccga cgagcttctc cgcgaagctg gcgcgtaagg acgtgggtct
     9301 cgtcctcgac gccgccgaag ctcagagcct gcaactgacg atagccgagg ccgtcgccgc
     9361 ccgcttcgac gaagccatag aggcaggata cggggaagca gacatgtccg ccatctacga
     9421 gtcggccgga gtcgggcggg cctgatccgc taccgggaag cccgggcgca gtagcggcgc
     9481 agcccttctt ctccctcgct catgacgctg tcgtggctgt ggcggtacat acgctcggcc
     9541 acgtagccca ggaggtttag ggccgggttg gtgacgcgca cgtaccagtt gaacgttacc
     9601 tgcgtgccgc ccccgtccgg actctccgtg aacagggttt cgaggacccc ggtcaggtct
     9661 cccgcaacga cctcgcgcag gcgccgctcg ggctccacgt ccgtgtagac gcagtgtatg
     9721 tggaatggcg gggcgagccg ggcgacctcc ggcgcctgat gtacctggaa agatacggtg
     9781 ctgccgacgc gcagttcgtc ttcatcctgc tcgtccgcca gctccatcga aggccaccac
     9841 tgccgcagcg cttctcgcga ggtcatcgcc tcgtagactg ccggggttgg cgcggggatg
     9901 gaccactccg tgcgccagtc gtaggtcttg atcagtcggt ttccggccag caatcccgcg
     9961 acggctccga tgaggcccgg tacgacgaaa tgtgcggcgc ttgagttctt catcgaacga
    10021 acctttctat tgactgttgc cgggtggatg cgccggatgc cggcgttggg tctcggccag
    10081 cgcctcggga cggtagaagc ggtcgtagaa gtccgggtcc aggtggtggg cctggtatgc
    10141 aagcaccgta cggaacgggc cgctgttggc cgggaaacgt ccgtaccggt tgtagacata
    10201 ctcgcagtag gagatcgtgg cggcgattgc ttcttcggag tagggaggga tgcctgcctg
    10261 tatccgtgct ccgtcttgcc aggccgtggc ggcgcctccg tcacggaacg tgccctctcc
    10321 ctgggcgtac ttgtagtcca cgaaagccag cacggcctcc ttcatggtcc ggtagtaggg
    10381 cgggcagaag ggtttgatct ggacttcacc tccccgttcc aaacctaccg ccgtgggcac
    10441 gggcaagtct ttgttcaatg cctttagcaa cctcttcgtc acagctccgg ccccgatggt
    10501 gcgggagaaa gggacctcct gcatgcgaaa gccgagagcc tgaaaccagg tgaagggatg
    10561 agccgcaaag tgagggaaac cgcccagccc caacgcctgg gtcattaggg ccaggttctg
    10621 caacatcccg ccctgctcta tggccccgaa ctcatagagc caggtctcga taaacccgac
    10681 cgtggcaaag cggccctctc tggggtcgtc gtgcagatat ccgcctctgg agcgggcgaa
    10741 acgggcgatg cccggcggtt tgaagcggtt acgctcgtcc accacgaagt aggcgaactc
    10801 ctcgccaaag gccgagagca ggatattgat atagatagcg gtgcattcgg caatcggtag
    10861 aaagtaagtc gtgccggtca ggttagccga ccactggttg aaaggcggca cgaagggggg
    10921 ctcccgtggc acgtccagcc gcttatcgga gatccggatc cggctctttt cataaagctc
    10981 caccagcttg tgttctctgg cggcgcggga caggctggcg atctcggctc ggggatggtc
    11041 ctgcggacgc ttcaacatcc acactccttc atcattgatg acgaagaggg ttatgtagtg
    11101 catggcatcc ccgctcggga ccgtgcggcc aacgaagtgg gtcatgatat tgccgccgcc
    11161 cgcgtctggc gcctcgccgc tctcgtaggg tagctcggcc agggcgtaac cggtgatgcc
    11221 gcaccccgcg aaggcgagag ctgcctcctc ttcgaggctc aacggctcgg gcgggcgtct
    11281 gctctggtag gcgagcggcc ctccgttcag ggtcatgccg ccgccgaagc gccgcgagcg
    11341 gcgttctatc agggcttcca gaagcgggta gcgggccaga tcgtcatacg attcgtgaat
    11401 cctcgcggct gtttcgctgg ttggttctcc cggtctcatg ccgctctcct ttccagtacc
    11461 tctttcaagc gccggagcgc ctcggcggac tcagcctcga tccggcgtcg aaacaccacg
    11521 cggtcgagca gcgccagcat cgcgttgggc atggagtaga cgaactcgcg ctcgaaggcc
    11581 gtgccgccgt ctcgcggcgt cagcgcgtag gtgatcttcc cgctgccgcc gctctccgtg
    11641 cggctctcga tcacccacct gcggggagct tcccgctcgc gcaccgtcca taccaccgtc
    11701 ccgcgccttc ccgcaaccag gaacctctcc gttacctgct cgcccggctt cagcgaatgg
    11761 tccgtcgcgc cgctaacacc gagtgaagaa gggtgccatc gcggccagtt gcctggcgtg
    11821 gtcgcgtagt cgaagacctg ctcgatgggc atctggatct gaacggtgtt gtatacgcgg
    11881 gccatagctg gctcctctac gtttgcctta cgagtcccgt ccgcgcaaca gcgccagtcc
    11941 cagccagagg tagaagagcg ggttgatcag gaacccgctt agtagcgcgg gaaccacgat
    12001 cagcgggctc gccgggtcga ggaagatcaa gcgtcccagg tacaacgcca cgagcagtac
    12061 agccgacgcg tatcccagat aacccaacct ctctgggaac tgtccgccgc gtacgatcag
    12121 ccacgcgacg ataagcaagg cgatgcctgc caccccaaaa gtgagcaggc cgcgagggtc
    12181 tatcgggttc ggcaggtctg ggatggaagg tggcgggttg acggcgttcg ccaggtcgta
    12241 gccgccgtgc gccgccgagc ccagagcgcc cgccatgccc agcagcagcg cccagagcgc
    12301 gaacgtcgcc tccgtccggc gtagccgctc gtagaccgcc accagtacgg ctgtggtgag
    12361 caggccgcct aacatgagaa agagcccgct caggagcgtg ttttgcagga cgacgaacgc
    12421 gacggcgtag aggaaaccgg tgacgcccgt caggaccgcg caggtcccgg cgaagcgctc
    12481 gaaaggtatt gaccgccgcg acgggaccgc tccgctctct actgttctgg tcgtcatcgc
    12541 gcactctcct tgttccttta gaatctcata gctcgggcaa gaggccgatg gtccgcagga
    12601 gtccggccac gtcccagatg tacagtccgc ggatgacctg tccgttttgc accacgagtt
    12661 gggaagcgcc gtgcacggcg atcttcttcc ccgtcggggg tatgttcatc agcttgccct
    12721 cgtgcgtgcc gtgtgccgtc cagaagagcg cgacacgggc gccctggacg ataatctcat
    12781 cctgggtgaa gctcagatcc gggaaggccc gcaggtagcc ggacatgtgc cgggacgcgc
    12841 cctgtggtcc gtggtacggc atggcctgtc ctacgtccgt tccctcatat tccggggcat
    12901 agaaatcctg cacgcgcccg gcatcgtgag agttccacgc ctccaccaac tcccggacga
    12961 actgactgat ctcttccccc gccggaccaa aaccggaacc gggtatttgg atttcaccgt
    13021 tgggatctag cgtcggcaag ctgtcgcacc tcttccttta ctggtcaata cggctccggt
    13081 agaaatatcc tggacagttc atccacgcta tccggctccc tccaggtccg ct
//
LOCUS       DASSMB010000024         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1145949, whole genome shotgun sequence.
ACCESSION   DASSMB010000024 DASSMB010000000
VERSION     DASSMB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145949"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFJ72_01925"
     CDS             <1..880
                     /locus_tag="VFJ72_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7478252.1"
                     /translation="CLRKVESPRIWEQFEAQGITHYCAAPTVQIGIVNDDAAHKLDTP
                     VTAAIAGAPPSPTLLGGLLELNINPMHIYGLTETYGPMTTSGVHREWEDMDLEERARM
                     MARQGQGYVTADLVRVVDEDMNDIEQDAETMGEIVMHGNMVAKGYLDNEEATEEAFEG
                     GWYHSGDVAVWHPDGYIEIRDRSKDVIVSGGENISTIEVEQAVARHPAVLEAAVIAIP
                     DEKWGERPKAFVTLKKGKEATEQEIIDYCKERIARFKAPAAVEFPEELPKTSTGKIQK
                     FVLRDKEWEGRDKKVN"
     gene            888..1574
                     /locus_tag="VFJ72_01930"
     CDS             888..1574
                     /locus_tag="VFJ72_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET7478253.1"
                     /translation="MPKLHILKVFVGENGAGGNPLGVFLDGQEIPEDERQKIAADLGF
                     SETVFVNDAERGVLRIFTPSTELPFAGHPLVGTAWLLKREGYEVPALRPPAGEVPVRE
                     DRGLTFISGRPEWAPGFEHVQLDSPADVDALTGPPDGHDLAGVWAWEDEEAGRVRVRV
                     FAPRVDVEEDEATGAHAIRLADILGRRITIHQGEGSLILAEPKPDGSVEIGGRVEPVE
                     TKEYRRKETP"
     gene            1571..2362
                     /locus_tag="VFJ72_01935"
     CDS             1571..2362
                     /locus_tag="VFJ72_01935"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET7478254.1"
                     /translation="MIEAQKSYEHILFERDGAVAYVTMNRPKKRNALSVAHMQELNAC
                     FKAVGEDREASVVVLRGEGPAFCAGHDLSEMIGRDPDTYRHIFDVCCELMETIQSIPQ
                     PVIAQVHATATAAGCQLAATCDLVVASEEAKFATPGVRIGLFCSTPMVALSRAVGQKK
                     SMEMLLTGDFISAEEARAEGLVNKVVPADELEKATRALADKISEASPLVVGIGKQAFY
                     RQLEMPTEQAYAYTKEVMSFNATFADAQEGMCAFLEKRKPEWKGR"
     gene            2466..>2948
                     /locus_tag="VFJ72_01940"
     CDS             2466..>2948
                     /locus_tag="VFJ72_01940"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET7478255.1"
                     /translation="MRNLAPSLSPAYETNRGHAYLGDSLKVMSCLPDSCVNLVMTSPP
                     FALTFKKEYGNKSQADYVSWFVEYAREMYRLLPEDGSFVLDLGGAWNKGTPTRSLYHY
                     KLAIALCEEVGFHLAQDFFWFRPAALPVPAEWVTVRRIRVKDAVNYVFWFSKTPWPKA
                     S"
BASE COUNT          614 a          889 c          947 g          498 t
ORIGIN      
        1 gtgtctcagg aaggtcgagt cgccgcgcat ctgggagcag ttcgaggcgc aggggatcac
       61 gcactactgc gccgcgccca ccgtccagat cggcatcgtc aacgacgacg ccgcgcacaa
      121 gctcgacacc cccgttaccg cagcgatagc cggagcgccg ccctccccca ccctgctcgg
      181 tggactgctg gagttgaaca tcaaccccat gcacatctac ggcctcaccg agacctacgg
      241 cccgatgacc acgagtggcg tgcaccggga gtgggaggac atggacctcg aagagcgggc
      301 gcggatgatg gcgcgtcagg gccagggcta cgtcacggcg gacctggtgc gcgtcgtgga
      361 cgaggacatg aacgacatcg agcaggacgc cgagacgatg ggcgagatcg tgatgcacgg
      421 caacatggtc gcgaagggct acctggacaa cgaggaggca accgaggaag cgttcgaggg
      481 cggctggtac cactccggcg acgtggccgt gtggcacccg gacggttaca tcgagatccg
      541 ggaccgcagt aaggacgtga tcgtctccgg cggcgagaac atctcgacca tcgaggtgga
      601 gcaggccgta gcccgacacc ccgccgtcct cgaagccgcc gtcatcgcca tccccgacga
      661 gaagtggggc gagcgtccga aagcgttcgt gaccctgaag aaaggcaagg aggcgacgga
      721 gcaggagatc atagactact gtaaggagcg gatcgcccgc ttcaaggcgc ccgccgccgt
      781 cgagtttccc gaggaactcc cgaagacctc gaccggcaag atccagaagt tcgtcctgcg
      841 cgacaaggag tgggaaggcc gcgacaagaa agtaaactag tagcagtatg ccgaagctcc
      901 acatcctgaa ggtcttcgtc ggcgagaacg gcgccggtgg caaccctctc ggcgtcttcc
      961 tcgacgggca agagataccg gaggacgaac gccagaaaat cgccgcggac ctcggtttct
     1021 cggagacggt cttcgtgaac gacgccgaac gcggcgtcct ccgcatcttc acaccgagta
     1081 cggagcttcc cttcgcgggc catcccctgg tcggcacggc ctggcttttg aagagggaag
     1141 gttacgaagt accggcgctc cggcctccgg cgggcgaggt ccccgtccgg gaagaccggg
     1201 ggctcacatt catctccggg cgtccggagt gggcgccggg gttcgagcac gtccagttgg
     1261 attctcccgc cgacgtggat gctctcacgg gaccaccgga cggtcacgac ctcgcgggtg
     1321 tgtgggcgtg ggaggacgag gaggccgggc gcgtccgggt gcgggtcttc gcgccgcggg
     1381 tcgatgtcga ggaggacgag gcgacggggg ctcacgccat ccggctagcc gacattctgg
     1441 ggcgtagaat tacgatccac cagggagaag gctccctcat actggccgag ccgaagcccg
     1501 acggcagcgt ggagatcggc ggtcgtgtgg agccggtcga gacgaaggaa taccgacgca
     1561 aggagacacc atgatcgaag cccagaagtc ctacgagcac atcctcttcg agcgggacgg
     1621 cgcggtagcc tacgtgacca tgaaccgccc gaagaagcgc aacgccctct cggtagcgca
     1681 catgcaggag ttgaacgcct gcttcaaggc cgtcggcgag gatcgggagg catccgtcgt
     1741 ggtgctgcgc ggcgagggac cggcgttttg cgcggggcac gacctctcgg agatgatcgg
     1801 acgcgacccc gatacctacc gccacatctt cgacgtctgc tgcgaactga tggagaccat
     1861 ccagtccatc ccgcagcccg tcatcgccca ggtccacgcg accgcgacgg ccgccggatg
     1921 ccagctcgcc gccacctgtg acctcgtggt ggcctccgag gaggcgaagt tcgccacgcc
     1981 gggggtcagg atcggtctgt tctgctccac gccgatggtc gccctctccc gcgctgtcgg
     2041 ccagaagaag agcatggaga tgctcctgac cggagacttc atctccgccg aagaagcccg
     2101 cgccgaaggg ctcgtcaaca aggtcgtacc ggcggatgag ctggaaaagg caacccgcgc
     2161 cctggcggat aagatctccg aggccagccc gctcgtcgtc gggatcggga agcaggcgtt
     2221 ctaccggcag ctagagatgc cgacggagca ggcctacgcc tacaccaagg aggtcatgtc
     2281 cttcaacgcc accttcgccg acgcccagga gggcatgtgc gctttccttg agaagcgcaa
     2341 gccggagtgg aagggtcgtt aaagacagac tttttcgtcg taccttacat agggcaagta
     2401 acatttccag attggaattg gctgcgaact agagtagtct tcattggcat gggagataaa
     2461 ctttgttgag aaatctggca ccaagtctga gcccagctta cgaaaccaat agaggccacg
     2521 cctatctcgg agactcgctc aaagtgatga gttgtctacc agactcatgt gttaaccttg
     2581 tgatgacttc accgcccttt gcgctgactt tcaagaagga gtacggcaac aagtctcagg
     2641 ccgattatgt ctcctggttt gtcgaatatg cgagagagat gtaccgacta cttccagaag
     2701 atgggagttt cgtactggat ctggggggag cctggaacaa aggtacgcca actcgctccc
     2761 tctatcacta caaattggct atagccctct gcgaggaagt tggctttcat ttggcgcaag
     2821 actttttctg gttccgtccc gcggctcttc ctgtgccagc ggagtgggtt acagtacgca
     2881 ggatcagggt caaagatgca gtcaactacg ttttttggtt cagcaagact ccctggccca
     2941 aagcatct
//
LOCUS       DASSMB010000025         2329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1019797, whole genome shotgun sequence.
ACCESSION   DASSMB010000025 DASSMB010000000
VERSION     DASSMB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019797"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFJ72_01945"
     CDS             complement(<1..678)
                     /locus_tag="VFJ72_01945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7478256.1"
                     /translation="MTTRPQTPDLAALKERQRKIWTSGEYAKIGNPLVIMGESLCEAV
                     DLRAGNRVLDVATGSGNTALSAARRFCEVTGMDLAPESIEHARKRAEAEGMDITFEVG
                     DAEDLSYPDATFDVVLSTLGVMFCPDQEKAASELLRVTRAGGRIGLSNWSPGSFIGNM
                     LRIVGKHVPPPPGIKPPPLWGTEERLQELLGEGVSSLNTTRRSYTFRYPSAPYFVEYF
                     RNYYGPTV"
     gene            complement(733..2124)
                     /locus_tag="VFJ72_01950"
     CDS             complement(733..2124)
                     /locus_tag="VFJ72_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7478257.1"
                     /translation="MTDTRTAALEGSLIAGLQTSLRGDLIQPGDDGYDTAREVYNAMI
                     DKRPALIARCADVADVISAVNFARDHGLTPAIRGGGHNGAGLGTCDDGLVIDLSSMKG
                     IRVDPAERTVRVEGGATWGAVDHATHAFGLATPSGIISTTGVGGLTLGGGLGHLTRKC
                     GLTIDNLLAADVVLADGRFVTASEEENEDLFWAIRGGGGNFGVVTAFLFRLHPISTII
                     GGPTLWELEQAPEVMRWYREFIAEAPEDLGGFFAFLTVPPGPPFLEELHLKKMCGIVW
                     CYTGPKEEADAVFAPVLEVGTPALHGVSEMPYPALQGAFDGLYPPGHQWYWKADFITE
                     LSDEAIKEHMKYAEVPTLQSTMHLYPINGAAHRVGKNDTPWSYREANWGMVVAGVDPD
                     PANREKITGWARDYWEAIHPHSAGGAYVNMMMDEGQERVKAAYRDNYERLAQIKRRYD
                     PTNLFRMNQNIKP"
BASE COUNT          381 a          750 c          735 g          463 t
ORIGIN      
        1 taccgtcggt ccatagtagt tgcggaagta ctcgacgaag tacggcgccg aaggataacg
       61 gaaagtgtag ctgcggcgcg tcgtattcag ggaggagacg ccttcgccga gaagctcttg
      121 tagccgctcc tcggtacccc ataagggggg aggctttatg ccaggaggcg gagggacgtg
      181 cttgccgacg atgcgtagca tgttgccgat gaaacttccg gggctccagt tggacagccc
      241 gatcctgccc ccggccctcg tgacccgaag caactcgctc gccgccttct cctgatccgg
      301 gcagaacatc accccgagcg tggagagcac gacatcgaag gtagcgtcag gataagagag
      361 gtcctcggcg tctccgacct caaaggttat atccatacct tcggcctcag cgcgcttgcg
      421 ggcgtgttct atggactcgg gggccaggtc catcccggtc acctcgcaga agcggcgggc
      481 ggcggagagc gcggtgttgc cgctgcccgt cgccacgtcg aggaccctgt ttcccgcgcg
      541 caagtccacc gcctcgcaga gcgactcgcc catgatgacc agggggttgc cgatcttcgc
      601 gtactcaccg gaggtccaga tcttgcgctg gcgttccttg agagctgcca gatcgggggt
      661 ctggggtcgg gtggtcatgt acccttcctt cgtgttgtgt tcggtggact tggcactcta
      721 tcgttcactt ccctatggct ttatgttctg gttcattcgg aagaggttgg tcgggtcgta
      781 cctgcgcttg atctgcgcca gccgctcgta gttgtcgcgg taggcggctt tcacccgctc
      841 ctggccttcg tccatcatca tgttgacgta ggcgcctccg gcggagtgcg gatggatcgc
      901 ctcccagtag tctcgcgccc agcccgtgat cttctctctg ttagccgggt cggggtctac
      961 gccggccacg accatccccc agttcgcctc gcggtagctc cacggcgtgt cgttcttccc
     1021 aacccggtgc gccgccccgt tgatcgggta gaggtgcatg gtggattgca acgtgggcac
     1081 ctcagcgtac ttcatgtgct ccttgatggc ttcgtcggag agctcggtga tgaagtcggc
     1141 cttccagtac cactgatgac ccggagggta gagcccgtcg aaggcgccct gcagagccgg
     1201 atagggcatc tcactcacgc catgcagcgc cggcgtacct acctccagga cgggcgcgaa
     1261 caccgcgtcg gcctcctctt tcggcccggt gtagcaccag actatgccgc acatcttctt
     1321 caggtgcagt tcttcgagga agggcggacc cggaggcacg gtcaggaagg cgaagaagcc
     1381 gcccaggtct tccggggcct ccgctataaa ctcccggtac caccgcatga cctccggggc
     1441 ctgctccagt tcccacagcg tcggcccgcc gatgatggta cttatggggt gcagcctgaa
     1501 gaggaacgcg gtaacgactc cgaagttgcc cccgccgccc cgtatcgccc agaacaggtc
     1561 ctcgttctcc tcttcgctgg ccgtaacgaa ccgtccatcg gccagaacca cgtccgcagc
     1621 cagcaggttg tctatcgtaa ggccacactt gcgcgtcagg tggccgaggc cgccgccgag
     1681 ggtgaggccg ccgacgccag tggtcgagat gatgccgctg ggcgtggcca ggccgaaggc
     1741 gtgggtagcg tggtccacgg cgccccaggt cgctccgccc tcgactcgga cggttcgctc
     1801 tgccggatct acgcggatgc ctttcatgga agagagatct atcaccaggc cgtcgtcgca
     1861 ggtacccagt ccggctccat tgtggccgcc gccgcggatg gccggggtga gaccgtgatc
     1921 acgggcgaag ttcacggcgg agatcacgtc cgcgacgtcg gcgcagcgcg cgatgagcgc
     1981 cgggcgcttg tctatcatcg cgttgtagac ctcgcgcgcg gtgtcatagc cgtcatcgcc
     2041 gggctggatc aggtctcccc gcaaactggt ttgcagaccc gcgatcaacg atccttctag
     2101 cgccgcagtc ctggtgtccg tcatggtttc ctcctcgtct gtcgtgtaac tcttcattca
     2161 gataggagcg gggagtcctc gaagccgtgc tctccctgca cctcaagagc ctggcgatgt
     2221 gcatggatct cgggatcaag aacctggagg ccaaccagct catcaactgc ggaaggctgt
     2281 acctgagcct cggtgccccg gaagacgccc tggagcactt ccggcacgc
//
LOCUS       DASSMB010000026         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_893702, whole genome shotgun sequence.
ACCESSION   DASSMB010000026 DASSMB010000000
VERSION     DASSMB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893702"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..664)
                     /locus_tag="VFJ72_01955"
     CDS             complement(224..664)
                     /locus_tag="VFJ72_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7478258.1"
                     /translation="MFEGFTERSHEVVALAREEARHLNHGYADTEHILLGLRREHGSV
                     AATVLSSFGVELDEVREEIETIVGQGERPVRGRVDLTPRAKKVLEPAQLEARQLANDH
                     VVPEHLLLGLAREPEGVAARILDSRGIRRDEIQRRVLRALGTGE"
     gene            845..1306
                     /locus_tag="VFJ72_01960"
     CDS             845..1306
                     /locus_tag="VFJ72_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HET7478259.1"
                     /translation="MDWDIVFWAALAALAFAGELLTISFFLLFFSIGALAALALSLAH
                     LPLAVQTAGFVAASILSMVILRPALLNRLALRTGERYVSSNEVTGKRGVVTSDIEPGD
                     KGMVRIGNGEFWTARSFHPDERLERGTKVRVLDTDGLTALVESVEIEGGER"
     gene            1306..2313
                     /locus_tag="VFJ72_01965"
     CDS             1306..2313
                     /locus_tag="VFJ72_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7478260.1"
                     /translation="MDVSIGLILLGILAFVVFLVAARSIRIIPQSRVAIVQRLGRYHR
                     TAESGLTFVVPIVDKMLPKTDLREQVIAFQPQAVITNDNVGMQISTVVYYQVVDARAS
                     EYEVANFHLAMEQIAQTTLRNVIGNLILDKTLTSRDEINGKLRTILDEVTEKWGIRVT
                     RVELKEITPPRDIQQAMEKQMQAERTRRAAILTAEGDKRAAILKAEGEKESSILRAEG
                     DQRSAVLRATGEAEAYRNVQAAQIEMTGKLFERLETSDLSPESLRYLYLKALPEMAKG
                     PASKLFVVPSEIQDLAGTIGALAGGASMAGEDGSKRGEKQRGLPLPPNGEIERKSLES
                     Q"
     gene            2567..3718
                     /locus_tag="VFJ72_01970"
     CDS             2567..3718
                     /locus_tag="VFJ72_01970"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7478261.1"
                     /translation="MITDTLTEAPRLSFDDLPISRQIHRAVTDQGWTSPTPIQALALP
                     ELLEGEDVVGLAQTGSGKTAAFSIPLIEGLDRKKRGVQALVLVPTRELASQAAAEISN
                     LSRHEPVRPLVLCGGTNIGPQIKALKGRSHPVVVGTPGRILDHLQRGTLKLGGVRYLV
                     LDEADRMLDMGFAPDVGRILSHTPDERQTALFSATMPGEIRGMVKRHMRSPKWLAVES
                     EAPTVDTVEQVYYRVDGRDKTRALRSLIDAEEKPLAIVFRRTKHGATKLHRQLERSGY
                     SAGLLHGGKTQSQRDKALAAFAGGRTRILVATNVAARGIDVPNISHVVNYDLPEDVET
                     YVHRIGRTARAGKDGIAATLVGETEVKEFDKIKRGLPVPVRESKLPLSA"
BASE COUNT          694 a         1167 c         1231 g          671 t
ORIGIN      
        1 cctcggaggt cgaggtcctg gacccggacg aggtagatct tgccttcgac cacgcgaaga
       61 tcatcgccga cgcccgcgac ctggacttat gaatcggcta ccgcgcaggc ggcaacgcag
      121 gcggccaagt ttagcttaaa tcggccttgt ctcattaaac agcttgcgga aacgtcgtat
      181 tttggagagg gtaagcagca cgcgattctc tatttggcct gcctcactcc ccggtgccaa
      241 gcgcgcgcaa gactctgcgc tggatctcat cgcggcgaat cccgcgactg tcgagaattc
      301 gcgctgctac gccttcgggc tctcttgcca gtccgagtag caagtgctct ggtactacgt
      361 gatcattggc gagttgtcgc gcctcaagtt gcgccggttc cagcaccttt ttagcgcgcg
      421 gggtgagatc tacccgtccg cgtaccggcc tttcaccttg gccgacgata gtttcgatct
      481 cttcgcgtac ctcgtccaac tccacgccga acgatgagag taccgtggcc gcgacgctcc
      541 cgtgttcccg ccggagtccc agcaggatgt gctcggtgtc cgcgtagccg tggttgaggt
      601 gtcgggcttc ctcccgcgcc agggccacga cttcgtggga gcgttccgtg aatccctcga
      661 acaacctctc atcatctgag cccgttgcgg ggtgagagct tctcctgagc catccgaaca
      721 cgtgcagcgt cccctctttg aggttttcaa ctctccgtgc gccagcttgc ggatcattgt
      781 acatacggat acgccgcgcc tgccgcgggt ggtatagaat ccacttgaag gaggccgcaa
      841 aggcatggat tgggacatcg ttttctgggc tgcgctggcg gctctcgctt tcgccgggga
      901 gcttctgacc atctcgttct tcctgttgtt cttctcgata ggcgcgctgg cggctctcgc
      961 tttgtccctg gcgcatctac cgctcgccgt gcagacggcg gggttcgtcg cggcttcgat
     1021 cctgagcatg gtgatcctgc gtcccgcgct cttgaaccgg ctggctctca ggaccggaga
     1081 gcggtatgtg agcagcaacg aggtcacggg gaagcgcggg gtcgtcacga gcgacatcga
     1141 gccgggggac aagggcatgg tccgcatcgg gaacggcgag ttctggacgg cgcggtcgtt
     1201 tcacccggat gagagactgg agagaggcac gaaggtgcgg gtgttggata cggatggtct
     1261 cacggcgctg gttgagtcgg tggagatcga aggaggagag aggtaatgga tgtgagcatc
     1321 ggtcttatat tgttagggat tctggcgttc gtggtgttcc tggtggcggc gaggagcatc
     1381 aggatcatcc cccagagccg ggtcgcaatc gtgcagagat taggacgcta ccaccgcacc
     1441 gccgagagcg ggctgacgtt cgtagtgccc atagtggaca agatgctgcc gaagacggac
     1501 ctgcgggagc aggtcatcgc gttccagccg caggcggtca tcaccaacga caacgtgggg
     1561 atgcagatct cgacggtcgt ctactatcag gtcgtggacg cccgggcttc cgagtacgag
     1621 gtcgccaact tccacctcgc gatggagcag atcgcgcaga cgacgctgcg gaacgtgatc
     1681 ggcaacctga tcctcgacaa gacgctgacc tcccgcgatg agatcaacgg caagctccgc
     1741 accatcctgg atgaggtgac ggagaagtgg ggcatccggg tgacgcgggt ggaactcaag
     1801 gagatcacac ctcccagaga catccagcag gcgatggaga agcagatgca ggccgagcgg
     1861 acccgccgcg ccgccatcct gaccgctgag ggtgacaagc gggccgccat cctgaaggcc
     1921 gagggcgaga aggaatcttc gatcctgcgc gccgaggggg atcagcgctc cgccgtcctg
     1981 cgcgctactg gcgaggccga agcctaccgc aacgtccagg ccgcccagat agagatgacc
     2041 ggaaaactct tcgagcgcct cgaaacctcg gacctctcgc ccgagtccct gcgctacctg
     2101 tacctcaagg ccctcccgga gatggcgaag gggccggcca gcaagctctt cgtcgtcccg
     2161 tcggagatac aggacctcgc agggaccatc ggcgcgcttg cgggcggcgc gagcatggcg
     2221 ggcgaggacg gctcgaagcg cggggagaag caacgcggcc tgccgctccc gccgaacggc
     2281 gagatcgagc gaaagagcct ggagagtcag tagcttcagg agcgccgcaa cgagccgggc
     2341 cgtgaacgcg gtccggctcg ttgcgtttga ctacgggcgc gcggcaggta tactcgccgg
     2401 gctacgtaat cgagaacata cgtccacgat tatcgagatg ccgaacttct agcgcgtctc
     2461 ttttttgtgc ctgtgcttat cctctcccgt cgagtggtcg cccatgacgt acatactccc
     2521 tatggtggcg gctgctaaac gcgacaagag gaaggaaaca cccgttttga tcacagatac
     2581 gcttaccgag gctccgcgcc tctctttcga cgatctccca atctccagac aaatccaccg
     2641 ggccgtcacc gatcagggtt ggacgagccc gaccccgatc caggcgctgg cgctacccga
     2701 gcttctggag ggggaggacg tcgtgggtct cgcgcagacc ggcagcggca agacggccgc
     2761 tttctcgata cccctgatcg agggtctcga ccgtaagaag cgaggcgtgc aggcgctggt
     2821 gctggtgccg acgcgggagc tcgcgtcgca ggcggcggcg gagatctcga acctctctcg
     2881 ccacgagccg gtccgtccgc tggttctgtg cggcggaacg aatatagggc cgcagatcaa
     2941 ggccctcaag ggaagatctc acccggtcgt cgtcggcacg ccggggcgca tcctcgacca
     3001 tttgcagcgc gggacgctga agctcggcgg cgtgcgctac ctggtcctgg acgaggccga
     3061 ccggatgctc gacatgggtt tcgcgccgga tgtcgggcgt atcctctcgc acacgcccga
     3121 tgagcgccag acggcgctgt tctccgccac gatgcccggt gagatccgcg gtatggtgaa
     3181 acgccacatg cgctccccga agtggctcgc ggtggagtct gaagctccca cggtagatac
     3241 cgtcgagcag gtctactacc gggtggacgg acgcgacaag acgcgcgccc tgcggtccct
     3301 gatcgacgcg gaggagaagc ccctggcgat tgtgttccgc cggaccaagc acggggctac
     3361 gaagctgcac cgccagctag agcgaagcgg ctacagcgcg ggtctcttgc acggcggcaa
     3421 gacgcagtca cagcgcgaca aggcgctcgc cgcgttcgcc ggaggccgga ccaggattct
     3481 tgtggcgacc aacgtcgcgg cccgcggcat cgatgtcccg aacatctcgc acgtcgtcaa
     3541 ctacgatctc ccagaggacg tggagaccta cgtgcaccgc atagggcgca cggcgcgcgc
     3601 cggaaaagac gggatcgcgg cgacgctcgt cggcgagacc gaggtcaagg agttcgacaa
     3661 gatcaaacgc ggactacccg tgccggtgcg cgagagcaag ctgccgctct ccgcctagaa
     3721 ccgcagtatc cgagccgcga tcccgtcttc gggtagagcg gct
//
LOCUS       DASSMB010000027        16920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1780400, whole genome shotgun sequence.
ACCESSION   DASSMB010000027 DASSMB010000000
VERSION     DASSMB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16920
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1780400"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VFJ72_01975"
     CDS             <1..414
                     /locus_tag="VFJ72_01975"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478262.1"
                     /translation="GRYMWSVGRLLWGFRCHDAVLRLDGERVVEARTILIAAALGTTY
                     GARFRLAPKAKLDDGLFDIVWSEEVNRAEVLRLIPAAFGDNLMEHPKVHLARASEVEI
                     TLEEEVPAHVDGELLEPTRAFRARVLPGALRVVAP"
     gene            520..1392
                     /locus_tag="VFJ72_01980"
     CDS             520..1392
                     /locus_tag="VFJ72_01980"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7478263.1"
                     /translation="MRAFVFPGQGGGVGEPAAEMLPTIRRVIGERVPDQVKFFARAAR
                     DADESRELAVRPDVVAGHSLGEYGAAYAAGCFDLETGIWLVARRDEFLAEAARKTPGG
                     MVAILKADPKEVEKVLGDTGGTFVANYNTPRQTVVSGLREALGEAVAKVRGRKIPLNV
                     SFAAHSPYVAAAGEKMREVIDSVEFRVPEVPIISAVDGAVLTSADAVREALKSQMVAP
                     VRWVKVVETLARMRVEEWIELGGGNVLTRMLRDFDLPSLKGITFEDLRARLSEQAQNL
                     LPRQNGSEQPLQPS"
     gene            1389..1727
                     /locus_tag="VFJ72_01985"
     CDS             1389..1727
                     /locus_tag="VFJ72_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478264.1"
                     /translation="MNVFFDVHGTLISGGKPRPHTREVFAALVESGHHVYLWSTAGPS
                     YARGAARLLGVEDLVFGYYSKYDHIPVTVDFFVDDHPDFADHSNGYAIRPFDGAPEDA
                     ELRKILEELT"
     gene            complement(1795..2904)
                     /locus_tag="VFJ72_01990"
     CDS             complement(1795..2904)
                     /locus_tag="VFJ72_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting membrane transporter"
                     /protein_id="HET7478265.1"
                     /translation="MKQRGLDEVKALSRDEAVEIMRQAGIVGAGGGGFPTYFKWKNPQ
                     PNLIVNCTESEPGYWGDKLLHRVFFDQFMELFEAMKAIFGIENVIMGVHFKDEEWFSR
                     YLENANGLYDVSLVADKYSMGEEKTLIKNIFKEDKEKWVPRRVELPDGNKRPGLPLDA
                     GHIVNNSETLLNIYNAFFLGKPVTTKYLQVFGPDVPLKIYECPLGASATELLKLSGVD
                     VEAEAGNLSVIDGGPYLNEMGIESLGEGDAFVRRTTNGFLVIPKGVASKEYADIKTKL
                     SEEKFTSLVGKVSGVNVPLGGRFLKPATALVSEGDEVSYGQKLGEPVDDGFSIGVWSS
                     MDGTVSSIEDEIVAISAGESFKQEAEAEAEAEAAR"
     gene            3018..3272
                     /locus_tag="VFJ72_01995"
     CDS             3018..3272
                     /locus_tag="VFJ72_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478266.1"
                     /translation="MKATTSATLAFEMKITRRTSNVLLAIGIYMLLTWGTRAFTFYTQ
                     LQDGTLVAPGVHFSLVVIGVAIGVYLTYLGIQGRKATRRS"
     gene            complement(3303..5240)
                     /locus_tag="VFJ72_02000"
     CDS             complement(3303..5240)
                     /locus_tag="VFJ72_02000"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HET7478267.1"
                     /translation="MREKFARPFGFLQRTFQQSPPGLRRGAAMLVDAAIVMESFAVAL
                     LFRFNGRVLSEWWLTFWPAAAVAAIVFVVLLRESGVYESILRYTGIYQGVRVASATSI
                     AAGILFIVDFALGPDGTGIMKFNPVPLGVVLVGSVLAYVQLVAVRLYPRVFYELSLRE
                     IGRQTRTVIVGTGESGVALAQHVWRTPGMNAQVVGFVSESPEDVGKQIEGVPVIGGLD
                     DMGTSISQRGVDQVIIATPQASREQMDSIWRTAVAASAEVKVMPDLGEFLAQGALKLR
                     ELQIEDLLGREPINIDLSALSEYINGKRVLVTGAGGSIGRELSRQISRLGPAKLILLD
                     RDESGLYYLNEELRREDFHGAETFVGDVTIPERVSFVFERYRPQLVFHAAAYKHVPMM
                     ELQGTEAIINNVFGTLNVARNAGAYGAGKFINVSTDKAVNPVNVMGATKRLAEILVRS
                     LADEYPETVYTSVRFGNVLGSRGSVVPTFRQQIEAGGPVTVTHPDMIRYFMTIPEAVS
                     LILQAGAMANSYGTYVLEMGRPVAITELARKMIEIMGAPNVKIRFVGLRPGEKLREEL
                     FEEGEQQGRTGHPMVFQLVSENNGTSDGSELYEIVEEMIFHARGQEAGKALSLLHKAV
                     PNYSTVDLPEELQEFRYPPQW"
     gene            complement(5296..6297)
                     /locus_tag="VFJ72_02005"
     CDS             complement(5296..6297)
                     /locus_tag="VFJ72_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478268.1"
                     /translation="MMAAAVAGGAAFLVAGGLVPLLVRFAVGRNLMDVPNLRSSHEVP
                     TPRLGGAAILIGTWIGIALLRPDGGWALLVTATLMGLVGLADDLFNLHFGWKAAVQVV
                     LASGLLLLYPPPVIMEAPGVLKVAAFLVGVVWISALTNAFNFMDGIDGFTGGVALVNM
                     LFLAVLVGDAGAFMPALAGATTGFLVWNMSPASIFIGDAGAYFLGFGLAAAALYAPAD
                     ASGSWTPLGFVAGALVFTPYLFDTAYTIFRRLRSGAGRDIFSAHREHIYQRITPAVSM
                     HRRTSNLYYGASVVAGFAALMVARGGTFTFAGALLALGCCAGLAALPYLVDTKHGSE"
     gene            complement(6294..7169)
                     /locus_tag="VFJ72_02010"
     CDS             complement(6294..7169)
                     /locus_tag="VFJ72_02010"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7478269.1"
                     /translation="MLVTGATGLLGGATVEELLCSGKYEVHCLVREGSPNASRLESLP
                     VGVFRGDVGDEKDLRRALSGMDALVHVAGIEYTPAVLRAMRRAGVDRLVLVGSTSVHS
                     RYEFRSGMRRTMEELVRGSGLRWTIVRPAMIYGSELDKNIHRLLRFLDRSPIFPMFGS
                     GENLWQPVYYEDCARGVFEALKRPVTIEKSYDLPGAEPLTYRDLVRTAAAALDRKPRI
                     VAIPLEPARRTLQLAETMRLSLPIKSEQVMRLREDKAYSYDRASRDLGYSPRAFSEGV
                     GLEVARLREIGLLKP"
     gene            complement(7267..7689)
                     /locus_tag="VFJ72_02015"
     CDS             complement(7267..7689)
                     /locus_tag="VFJ72_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478270.1"
                     /translation="MFLLASLVLLSTLISGCGGSGGAQSSQDNAKDKTAQKEPKKAKK
                     KNAPEVKIALGTVVFHNPDTQRFTLRPNKGENRIFRYNKNTKITLNGKEADPKDIEKG
                     LQAQAKYIVKDDLARVRKLDLISKDDSKNSSGGGETTG"
     gene            complement(7821..8825)
                     /locus_tag="VFJ72_02020"
     CDS             complement(7821..8825)
                     /locus_tag="VFJ72_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET7478271.1"
                     /translation="MKLTRVLKRVADLIGRWQPSSLREWLPSREMSFILARKDEEISR
                     LRKELIVARDDTRGVSPENVIWMFGVARTGSSWLAAMMAELERHATWYEPYVGDLFGV
                     AYQVRSPEWQRRRDDFVLSDRYRAAWLRSIRSFVLDGANARFPDLGSEGYLVIKEPNG
                     SLGAPLLTEALPESRVILLVRDPRDVVASLLDANRKNGWVSRHGAPGGGASLADADPD
                     AFVRQQANLCIGSLSKAKEAYDAHDGCKAIVRYEDLRGDALGELKRIYSVLDIPTKEA
                     QLARAVERHAWENVPAEKKGVGKFYRKAKPESWREDLTPEQARIVEEITVPLIHEFYA
                     "
     gene            complement(9003..9419)
                     /locus_tag="VFJ72_02025"
     CDS             complement(9003..9419)
                     /locus_tag="VFJ72_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478272.1"
                     /translation="MTILLLVMGLMLALGAGVALAGVGSVFVGTSQNDRLLGTRGDDN
                     INALQGDDFVNARGGDDVVDGGANHDRLQGWTGNDRLIGGTGQDVLLGGFGNDIIDAR
                     DNGGQDDINCGPGRDTLLADAADRDIALTSCERVLR"
     gene            9827..13219
                     /locus_tag="VFJ72_02030"
     CDS             9827..13219
                     /locus_tag="VFJ72_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7478273.1"
                     /translation="MNICTIVAKNYLAHARVLAESFKELHPEGTCYVLVVDETEDYVD
                     PAKESFELVTPSQLDIEDFEHMAAIYDVVELSTAVKPWFLSYLLNEVGMDRVAYFDPD
                     IRIFERLDRLEELIREYELVLIPHVTDPMPRDGKKPSEADILIAGTYNLGFIALERGP
                     RANRLLDWWAERLRTDCVVAPEMGYFVDQRWIDFVHGVMPGFYVLRDPGYNVAYWNLH
                     GRNLTRKQDRYYVNGEPLTFFHFSGYDPRNPDRLSKHQSRTDLGRGTALRRICDEYAE
                     ALFAQGFEKVRTWPYTYSVMPNGVKLDPGMHKLYRKGSEAGQLKGSIFTREGADRFVA
                     WLDEPAPVGGGQGVTRYLYEIYENRPDLRQAFPNLDGPDAPAFLEWARTHGGVEVPLS
                     HHPAVEGDGYRPAGAPNGVNVAGYFRSELGVGEVARQMTAAMETQGIPVATVGIATPH
                     SRQGHDYETDAVAPTYPVNLICVNADALPDFAAHAGLDLFKNRYSVGMWWWEVSEFPE
                     RFDKAFEYVDEVWVGSHHVADAVAKVSPVPVVKVTVPFSMPPVPEMSREELGLPEGFL
                     FLFSFDYHSVFERKNPLAVVEAFKKAFAPGSGASLAIKSINGDKHPEEHARLVAAAED
                     HPDIRVIDRYVSFAEKNAIAGACDCYVSLHRSEGFGFTMAEPMYLGKPVIATAYSGNM
                     DFMTDRNSYLVDYDIVPIGEGADPYPPTGEWAEPDVEHAASLMRRVFKNPEEAQERGK
                     RAARDIRRTQSPEAAGKAIAARLSHIRARLGAGAGEQTARTSAAQQHPGLKTGALENR
                     IMHTPTLPVTPSLPGRVMGFARRVALKLNWPFAMQQRTINDQLLTMIKGVDATLRDVT
                     GRLEKQGSRTVAGLEAQTKEIEALYPRVEAADRLIAETRALPYMANSPFETFEEPEAG
                     SVFGYEERNGSSGSAGTYDAFEDIFRGDEAFIRERQRRYLDILGGREPVLDVGCGRGE
                     FLDLLREEGIPYTGVDLDPDMVERCRRKGHENVEVADANSYLEKRLDDSIGVIFCAQV
                     IEHLPYEELLRFFELAERKLAPGGLFVAETVNPHSVPALKTFWVDLTHQHPVFPEVTL
                     ALCKINGFDKAYVFHPNGAGDVEKDRYETGEYAVVATKAERP"
     gene            13216..15165
                     /locus_tag="VFJ72_02035"
     CDS             13216..15165
                     /locus_tag="VFJ72_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7478274.1"
                     /translation="MSAGGDARRNGRDFADRLPKGLRGLVRLATVNPRLDRVVANQNR
                     LGEQVKRIDARSQQLTIQTRELDQKLQNANLAGMRETINSQKARIDQLEAQLSEMLAG
                     NAPVGKRELSERELRAEISKVDNWYHTIQLAPGHFTPGSHKSDLALAAMHVPEDLSGK
                     RVLDVGARDGFFSFVAEARGAEVLAIDAVAPNLTGFSVAAPLMDSSVRFETMNVYDLD
                     PETVGEFDAIFFLGVLYHLRDPMLALDRLWSVAKPGATVWVESHVADYGLVDPESGEW
                     RSLAAVAPDLVDVPMAQFYPDDLLANSTTNWWGPNLAGLRAMVEAAGFEILRTRFVGG
                     RGLVVGRKVEDEDTDFYREFDRGTATGEEGMAWESVKQWYNARPPAESKTALRRNREV
                     SPEEARAEVAKFENWYHRIEVAPGVVTPGVHDSEAALEAMEVPESFEGMRVLDVGARD
                     GYFSFVAEARGAEVLAIDAVALEHLQGFSTAAALLGSSVEYKTMNVYDVSPENVGQFD
                     AIFFLGVLYHLRDPMLALDRLWSVCRPGATIWVESHTIDHGFIDPATGEQKSFAAVAP
                     ALAKYPMAQFYPKGLLGGNQTNWWGPNLAGLEAMVRSAGFEVQRSRLVGGRGLVVGKK
                     VEDENTNFYRWYDRGVVTDKKDQRA"
     gene            15195..16043
                     /locus_tag="VFJ72_02040"
     CDS             15195..16043
                     /locus_tag="VFJ72_02040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7478275.1"
                     /translation="MGILRKLWQREDKDQEIPIPPLEMRQLVGPTDPAAFDNPTGELV
                     FPFLPESAFEAVFDYGCGCGRLARQLLQQKPRPKRYVGVDIHRGMIEWDQNNLTPVDR
                     NFQFHHHDVYNPSHAPTNSRRLTAPFEVEDKGFSLVIAWSVFTHIYEAQALHYLKEIA
                     RIMRPDGVFLSTWFLFDKREFPMMQRFQNTLFVNETDPTNAVIFDREWVKEAISQAGL
                     SIEEAVPPSIRGYHWRVTMRHKQPGDGPFEFPPDEAPYGSMPPPLMPQTTPPGDGQGG
                     QGRGGG"
     gene            16043..>16920
                     /locus_tag="VFJ72_02045"
     CDS             16043..>16920
                     /locus_tag="VFJ72_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7478276.1"
                     /translation="MPAFKRRLSLKLRRNLRLSLDVEGELPDLRNVANRLKGGKPAPA
                     SGTARRGAEPRPTGPMPTPNTQLSSEDVRVEISKVEHWYHQIEVSPGITTPGVNPSAN
                     QLAAMEVPADLSGMRVLDVGARDGYFSFVAEARGAEVLAIDAVAPNLTGFDTAAGLLG
                     SSVEYRTMNVYDVSPEEVGRFDAVFFLGVLYHLRDPMLALDRLWSVAKPGATIWVESH
                     TIDRGFVDPETKEVRDLSEVAPNLAGVPMAQFYPGDQLGGSVANWWGPNLAGLEAMVR
                     SAGFEVQRSRLVGGRGL"
BASE COUNT         3140 a         5552 c         5308 g         2920 t
ORIGIN      
        1 ggccggtaca tgtggtcggt cggtcggctg ctctggggat tccggtgcca cgacgcggtc
       61 ctgcggctgg atggggaacg ggtcgttgag gcaaggacca tactcatcgc ggcggcgctc
      121 gggacgacct acggggcgcg cttcaggctc gcgccgaagg cgaagctgga cgatgggctc
      181 ttcgatatcg tctggtccga agaagtcaac cgggccgaag tcctccggct aataccggcg
      241 gcgttcggcg acaacctgat ggagcatcca aaagtacatc tcgcgcgggc gagcgaagta
      301 gagatcacgc tggaggaaga ggtcccggct cacgtggacg gggagctgct ggagcccacc
      361 cgcgctttcc gggcgcgagt cctgccggga gcgctgcggg tcgtggcgcc gtagacgctg
      421 caacttttgg tcaagagcgg cgtactataa taagaacgtc tcttcgcgta aaatagcgcg
      481 gtcgtataga ttcaggatat ttcaagggtc aggtatcgga tgagagcttt cgtatttccg
      541 ggacagggtg gcggagtagg agagccggcg gcggagatgc tgccgaccat ccggcgcgtc
      601 atcggagagc gggttccgga tcaggtaaag ttcttcgccc gcgccgcgag agacgcggac
      661 gagagccggg agctcgcggt gcgccccgac gtggtagccg gccactcttt gggcgagtac
      721 ggcgcggcct acgccgccgg atgcttcgac ctggagaccg gcatctggct cgtggcccgg
      781 cgcgacgagt tcctcgcgga ggcggcgcgg aagacgccgg gcggcatggt cgccatccta
      841 aaggcagacc caaaagaggt cgagaaggtc ctgggggaca ccggcggcac gttcgtcgcc
      901 aactacaaca ccccgcgcca gacggtcgtc tccgggctgc gcgaggctct gggtgaggcg
      961 gtagcgaagg tgaggggacg caagatcccg ctgaacgtct ctttcgccgc gcactcgccc
     1021 tacgtggccg cggccggaga gaagatgcgc gaggtcatag actccgtgga gttccgggtg
     1081 ccggaggtcc cgatcatcag cgccgtggac ggcgcggtgc tcacgagcgc cgacgcggtc
     1141 cgggaggcgc tcaagagcca gatggtcgcg ccggttcgct gggtcaaggt cgtcgagacg
     1201 ctcgcccgga tgcgggtcga ggagtggata gagctcggcg gcggcaacgt gctgacccgg
     1261 atgctccggg acttcgacct cccctctctc aagggcataa ccttcgagga cctgcgcgcc
     1321 cggctctcgg agcaggccca gaacctcctg ccccgccaga acggaagcga gcagccgctc
     1381 cagccttcgt gaacgtcttc ttcgacgtcc acggaaccct catctccggc ggaaagcccc
     1441 gacctcatac aagagaggtc ttcgccgcgc tggtagagag cggccatcac gtctacctct
     1501 ggtccacggc cggtccctcc tatgcccgcg gcgcggcgcg actcctcggg gtcgaagacc
     1561 tcgtcttcgg ctactacagc aagtacgacc atatccccgt gacggtggac ttcttcgtgg
     1621 acgaccaccc ggacttcgca gaccacagca acggctacgc tatccgcccg ttcgacggcg
     1681 cccccgaaga cgccgagcta agaaagatcc tcgaagaact cacgtaaaga cgcacagcaa
     1741 aaaggccgga gcatggaacc ccggccctgc acagatcctt gagaatgcta accgctaccg
     1801 ggcggcttcc gcctcggcct cggcttcggc ctcctgcttg aaggactcgc cggcgctgat
     1861 cgccacgatc tcatcctcga ttgaggagac ggtgccgtcc atgctcgacc acacgccgat
     1921 ggagaaacca tcgtccacgg gctcgccgag cttctggccg taggagacct cgtcgccctc
     1981 ggagacgagc gcggtcgcgg gcttgaggaa gcgcccgccg agcgggacgt tcacgccgct
     2041 gaccttgccc acgagcgagg tgaacttctc ctcggagagc ttcgtcttga tatcggcgta
     2101 ctccttgctc gccacgccct tcgggatcac gaggaacccg ttggtcgtgc ggcgcacgaa
     2161 ggcgtcgccc tcgccgagcg actctatgcc catctcgttc aggtacgggc cgccgtctat
     2221 gacggagagg ttgccggctt cggcttccac gtccacgccg gagagcttca atagctccgt
     2281 ggccgacgcg ccgagcggac actcatagat cttcaacgga acgtcgggac cgaagacctg
     2341 caggtacttc gtggtgaccg gcttgccgag gaagaaagcg ttgtagatgt tcaggagggt
     2401 ctcggagttg ttgacgatgt gcccggcgtc gagcgggaga ccagggcgct tgttgccgtc
     2461 gggaagctcc acccggcgcg gcacccactt ctccttgtcc tccttgaaga tgttcttgat
     2521 gagcgtcttc tcctcgccca tcgagtactt gtccgcgacc aggctcacgt cgtagaggcc
     2581 gttcgcgttc tccaggtagc gggagaacca ctcctcgtcc ttgaagtgga cgcccatgat
     2641 gacgttctcg atgccgaaga tggccttcat ggcctcgaag agctccataa actggtcgaa
     2701 gaagaccctg tggagcagct tatcgcccca gtaacccggc tccgactcgg tgcagttcac
     2761 gatcaggttc ggctgcgggt tcttccactt gaagtacgtc gggaacccgc cgcccccagc
     2821 ccccacgata cccgcctggc gcatgatctc gaccgcctca tcccggctca aggcctttac
     2881 ttcgtcgaga ccgcgctgct tcatctcagc caagattctt tccctccgaa cactctgtat
     2941 acttcaggta tttacagtta caaactctgc tcaactatta ctaacaagta tactctctgt
     3001 gtaatcgcat cgctagtttg aaggcgacaa cgtctgctac actggctttc gagatgaaga
     3061 tcacacgccg cacctcgaat gtcctcctgg ctatcggtat ctacatgctc ctgacctggg
     3121 gtacgagggc gttcaccttc tacacccagt tgcaagacgg gacgctcgtc gcccccggcg
     3181 tgcacttctc gctcgtcgtg atcggggtcg ccatcggcgt ctacctgacc tatctcggca
     3241 tacagggccg caaggccacc cgccgaagct agaagggtaa gacctatcgg cctatcgcca
     3301 gcctaccact ggggcgggta gcggaactcc tgtagctcct ccggcaggtc gacggtagag
     3361 tagttgggga cggccttgtg gagcagagag agggcctttc ctgcctcctg gcccctggcg
     3421 tggaagatca tctcctctac gatctcgtag agttccgagc cgtcggaggt gccgttgttc
     3481 tcggagacta gctggaagac catcggatgg ccggtacgac cctgctgctc tccctcctcg
     3541 aaaagctcct cgcgcagctt ttcgccgggg cgcaggccaa cgaaccttat cttcacattc
     3601 ggggcgccca taatctcgat catcttccgg gccagctccg tgatggcgac gggacggccc
     3661 atctccagga cgtaggtgcc gtagctgttg gccatcgcgc cggcctggag gatcaaggag
     3721 accgcttcgg ggatggtcat gaagtaccgg atcatgtccg ggtgagtcac cgtaacgggt
     3781 ccgccggcct ctatctgctg ccggaacgtc gggaccacgg agccgcggct gccgagcacg
     3841 ttcccgaacc ggaccgaggt gtagaccgtc tccgggtact catccgccag gctgcggacc
     3901 agtatctccg ccagacgctt cgtcgccccc atcacgttca ccgggttgac cgccttgtcc
     3961 gtcgagacgt tgatgaactt cccggcgccg taggctccgg cgttgcgagc gacgttcagg
     4021 gtgccgaaga cgttattgat gatcgcctcg gtgccctgca actccatcat tgggacgtgc
     4081 ttgtaggcgg cggcgtggaa gaccagttgc ggccggtagc gctcgaacac gaagctcacc
     4141 cgctcgggga tggtcacgtc gccgacgaaa gtctcggcgc cgtggaagtc ctcgcgccgc
     4201 agctcctcgt tcaggtagta gagtccgctc tcgtcccggt cgagcaggat cagcttcgca
     4261 ggtccgagac gtgagatctg gcgactaagc tccctcccga tggagccgcc cgctcctgta
     4321 accagcaccc gcttgccgtt gatgtactcg gagagggcgc tcaggtcgat gttgatcggc
     4381 tcccgtccca ggaggtcttc gatctggagc tcccgcagct taagcgcgcc ctgcgccagg
     4441 aactcgccga ggtccggcat caccttcact tcggcggagg ccgcgaccgc ggtgcgccag
     4501 atactatcca tctgctcccg gctggcctgc ggcgtggcga taatcacctg gtccacgcca
     4561 cgctgcgaga tcgatgtacc catgtcgtcc agcccaccga tgaccggcac gccttcgatc
     4621 tgcttcccga catcctccgg cgactcgctc acaaacccaa cgacttgggc gttcatcccc
     4681 ggcgtgcgcc atacgtgctg cgccagcgcc accccggact cacccgttcc aactatgacc
     4741 gtccgcgtct ggcgtccgat ctcccgcagg gaaagctcgt agaacacacg aggatagagc
     4801 ctgacagcaa cgagctgcac gtaagccagc acagatccga caaggaccac ccccagcgga
     4861 acaggattga acttcataat tcctgtccca tcgggtccca gcgcgaagtc cacgataaat
     4921 agaataccgg cggctatgga ggtcgcgctg gcgacccgca cgccctggta gatacccgtg
     4981 tagcgcagga tactctcgta gactccactc tcgcgcaaaa gcaccacgaa gacgattgcc
     5041 gctacggctg ccgcgggcca gaacgtcagc caccactcgg agagcacgcg gccgttgaac
     5101 cggaagagca gggccacggc gaaagactcc atgactatcg cggcatccac caacatcgcc
     5161 gccccacgcc gcagacctgg aggcgactgc tggaaggtcc gttgcaagaa gccgaacgga
     5221 cgcgcgaatt tctccctcat cctcatcggc ccattgtaag actgatcggc ccctgtatcc
     5281 agtcaatagc cacttttatt cggagccgtg cttcgtatcc accagatatg gtagagcggc
     5341 gagccccgcg caacagccga gcgccagcaa tgcgcccgcg aaagtgaacg ttccaccacg
     5401 ggctaccata agagcggcga agcccgcgac gacgctcgcg ccatagtaca agttggaggt
     5461 gcgccggtgc atcgaaaccg cgggcgttat gcgctggtag atgtgctccc ggtgcgcgga
     5521 gaagatatcc ctgccggcac cgctccgcag acgccggaag atcgtataag ccgtatcgaa
     5581 gaggtacgga gtaaagacca gggcacccgc cacgaagccc agcggcgtcc agctcccgga
     5641 agcatcagcc ggcgcgtaga gtgccgctgc cgcaagtccg aagcccagga agtacgcccc
     5701 cgcatccccg ataaagatgg aggcggggct catgttccac accagaaacc ccgtggtggc
     5761 cccggccagc gcgggcatga aggctcccgc atctcccaca aggaccgcca ggaagagcat
     5821 attgacgagc gccacgcccc cggtaaagcc gtctatgccg tccatgaagt taaaggcgtt
     5881 ggtgagcgcg gagatccaga ccactcctac caaaaacgcc gcgactttca aaacgcccgg
     5941 cgcctccata attaccggcg gcgggtagag cagcaacagc cccgaagcga gcacaacctg
     6001 caccgccgct ttccagccga aatgcaggtt aaagaggtcg tcggcgagcc cgacgaggcc
     6061 catcaacgtc gcggtgacga gcagagccca ccctccgtcg gggcgcagga gcgcgatgcc
     6121 gatccaggtc ccgatcagga tagccgcccc gccgagccgg ggcgtcggaa cctcgtgcga
     6181 gctgcggagg ttcggtacgt ccatcaggtt gcggcccacg gcgaacctca ccagcagcgg
     6241 caccagcccg cccgctacca ggaaggcggc tccgccggcc acggccgccg ccatcatggc
     6301 ttgagcagcc cgatctctcg cagccgcgcc acctccagcc caaccccctc agagaacgcg
     6361 cgcggagagt atccgagatc ccggctggcc ctgtcgtaag agtaggcctt gtcctctcgc
     6421 agcctcatca cctgctccga ctttatcggt agagacagcc gcatggtctc cgcgagttgc
     6481 agggtacgac gcgccggctc gagcgggatc gcgacgatcc gcggcttcct gtccagcgcg
     6541 gcggcggcgg tccggacgag gtctctgtag gtcaacggtt ccgcgcccgg caggtcgtag
     6601 ctcttttcta tcgtcacagg gcgctttaga gcctcgaaga ccccgcgggc gcagtcctcg
     6661 tagtagacgg gctgccagag gttctcgccg gagccgaaca tcgggaagat gggcgagcgg
     6721 tcgaggaatc ggagcaagcg atgaatgttc ttgtccagct cggagccgta tatcatggcc
     6781 ggacgcacta tagtccaccg gagcccgctc ccgcgcacca actcctccat agtccggcgc
     6841 ataccggatc ggaactcgta cctcgaatgt acgctcgtgc tgccgactag cacgagccga
     6901 tccacacctg cccggcgcat cgctcgcagc accgcgggcg tgtactcgat accggcgaca
     6961 tgcaccaggg cgtccatacc ggagagcgcc cgccgaagat ccttctcgtc cccgacatct
     7021 ccacgaaaga cccccaccgg caacgactcc agccgcgaag cgttcgggct gccttcacgt
     7081 accaggcaat ggacttcata ctttccggaa cacaaaagct cctcaacggt tgcaccgccc
     7141 aagagcccgg tagcgccagt aacaagcaca ctcaacaacc catcacctcc agcaactcag
     7201 ccatcgcgcc gggctctcga aggaggtcta agtaagcgta aagacgctct ttcggaagac
     7261 gcctgcttaa ccggtcgtct ctcctccgcc agagctgttc ttagagtcat ccttgctaat
     7321 aaggtccagt ttccgtaccc gtgccagatc gtccttgact atgtatttgg cctgggcctg
     7381 aagtcccttc tctatgtcct ttggatcagc ctctttacca tttagcgtaa tcttcgtgtt
     7441 cttgttgtac cggaatattc tattttcacc cttgttcgga cgcagggtaa acctctgggt
     7501 atcaggatta tggaacacga cggtgccaag agctatcttg acctccggcg cgttcttttt
     7561 cttggctttc ttgggctcct tctgagcggt tttgtctttc gcgttgtcct gactactctg
     7621 ggctccgcca ctgccaccac aacctgagat cagggttgaa agcaaaacta gcgaggccag
     7681 tagaaacata accaaagacc tgcgagtcgt cattctacgg tccattgagg atctccttgt
     7741 tcgaaggctc gacttcgtca acctagcaca agatttgatg agctaccaag ggatttggtc
     7801 taacatgcca cctcagtcta tcaagcgtag aactcatgaa taagggggac agtgatctcc
     7861 tccactattc tagcctgctc cggagtgagg tcctcacgcc agctttcggg tttcgctttg
     7921 cggtaaaatt ttccgacgcc tttcttttcg gcggggacgt tctcccaggc atgtctctcg
     7981 accgcccgcg cgagttgcgc ctctttggtg ggaatgtcga gtacggagta aattctcttc
     8041 aactcaccca gggcgtcacc gcgcaagtcc tcgtagcgga cgatcgcctt acaaccgtcg
     8101 tgagcgtcgt aggcttcctt cgccttgctg agactcccta tgcacaggtt tgcctgctga
     8161 cgcacgaagg cgtccgggtc ggcgtccgca agagaggctc cgccgccggg cgcgccatgc
     8221 ctggaaaccc aaccgttctt cctgttggca tccagaagcg aggcgaccac gtcgcgcggg
     8281 tctcggacaa gcaggatcac gcggctctcc ggtagggcct cggtaagaag cggcgcgccg
     8341 agagagccat tgggttcctt gatcaccagg taaccttcgg aaccgaggtc tgggaaacgg
     8401 gcgttcgcgc cgtcaagcac aaaggaccgg atggagcgga gccacgccgc ccggtagcgg
     8461 tcgctgagca cgaagtcgtc ccgcctgcgc tgccattctg gggagcggac ttggtatgcg
     8521 acaccgaaca ggtcgcccac gtaaggctcg taccaagtgg cgtgtctctc cagctcggcc
     8581 atcatcgcgg cgagccaact gctcccggtg cgggcgaccc cgaacatcca gatcacgttc
     8641 tctggactca cgccgcgagt gtcgtccctc gcaacgatca actccttacg caggcgggag
     8701 atctcctcat ccttccgcgc gagtatgaag gacatctcgc gggaggggag ccactctctc
     8761 agagaggatg gttgccaccg gccgatgaga tccgctactc ttttgagaac ccgcgtcagc
     8821 ttcatgcctc ctggcatctg tggatcgtag aaaaccaccg aaacagcttg gatttaatca
     8881 gtcgagggtc tcagcttcaa gcagttccac ctccgtaaag gcgtcatctg accaaagcga
     8941 agagcggagc cgacaagagt ttttggctcc gctccagtcc gagagcctta gctcgcggtg
     9001 ggttatcgca gcacgcgctc gcagctagtg agcgctatgt cccggtccgc cgcatcggcg
     9061 agaagtgtgt cccgtcccgg accgcagttg atgtcgtctt ggccaccgtt gtcgcgcgcg
     9121 tctatgatgt cgttgccgaa tccaccgagc aatacatcct gtcccgtgcc accgatcagc
     9181 cggtcgttgc ccgtccagcc ttgcagccgg tcgtggttgg caccgccgtc aacaacatcg
     9241 tcgccgccgc gggcattcac gaagtcgtcc ccctgcaagg cgtttatgtt gtcgtcgcct
     9301 ctggttccca gtagcctatc gttttggcta gtgccgacga ataccgagcc taccccagct
     9361 agagccacgc cagctcccag cgcgagcatc agtcccatca ctagcaacag aatggtcatc
     9421 cgcctcattt cctttgctcc ttccgctcct ttttgggatc cgcgaggatc cgatttacct
     9481 ccgacatgtg ccattttggc tgttgcggag ggcgagatcg tcctccatat gcgtgatctt
     9541 ttcttagccc actttgcgta gcgttcgcac ggctcgactg ctgataaacg tcaagatccg
     9601 tagcgcggct gacccgggcg gcatcgtcgg ttgacaatgg gccgcgttga acctattctt
     9661 gcgacgatcc gatagcggtc ggcgcgagct ttcgagtccg ttcggagaaa agcaggagcg
     9721 atttgcggcg gttgctgttt ctttgcctac caacagaggt tgttgcagga gagacagtgt
     9781 cgcgcgacaa acgaaaagaa ccttttagcg agattggccg gtggtcatga acatctgcac
     9841 gatcgtagcc aaaaactatc tggcgcacgc ccgcgtactg gccgagtcct tcaaggaact
     9901 ccacccggag gggacgtgct acgtcctggt cgtggacgag acggaggact acgtggaccc
     9961 cgcgaaggag tccttcgagc tggtcactcc gtcgcaactc gatatagaag acttcgagca
    10021 catggcggcg atctacgacg tggtcgagct ctctaccgcc gtcaagccgt ggttccttag
    10081 ctacctgcta aacgaggtag ggatggaccg ggttgcgtac ttcgacccgg acatcaggat
    10141 cttcgagcgg ctggaccggc tggaggagtt gatccgggag tacgagctag tcctgatccc
    10201 gcacgtgacc gaccccatgc cccgcgacgg caagaagccc tcggaggcgg acatcctgat
    10261 cgcgggcact tacaacctgg gctttatagc cctggagcgt ggaccgcgcg cgaaccggct
    10321 cctcgactgg tgggccgagc gcctgaggac cgactgcgtg gtggccccgg agatgggcta
    10381 cttcgtggac cagcgctgga tcgacttcgt ccacggcgtg atgccgggct tctacgtgct
    10441 gcgagatccc ggctacaacg tggcctactg gaacctgcac ggccgcaacc tgacccgcaa
    10501 gcaggaccgc tactacgtga acggcgagcc gctgacgttc ttccacttct ccggctacga
    10561 cccgcgcaac ccggaccgcc tgagcaagca ccagagccgc accgatctcg ggcggggcac
    10621 cgctctgcgg cgcatctgcg acgagtacgc cgaggccctg ttcgcccagg gcttcgagaa
    10681 ggtccgcacc tggccctaca cctacagcgt gatgccgaac ggcgtgaagc tcgaccccgg
    10741 tatgcacaag ctctaccgca agggctccga ggcggggcag ctcaagggct ccatcttcac
    10801 ccgcgagggg gcggaccgct tcgtagcctg gctcgacgag cccgcgccgg tgggcggcgg
    10861 gcagggcgtg acgcgctacc tctacgagat ctacgagaac cgcccggatc tgcggcaggc
    10921 tttccccaac ctcgacggcc cggatgctcc ggccttcctg gagtgggcgc gcacccacgg
    10981 cggcgtcgag gtcccgctgt cgcaccaccc ggcggtcgag ggcgacggat accggcccgc
    11041 gggagcgccg aacggcgtca acgtcgccgg gtacttccgc tccgagctcg gggtcggcga
    11101 ggtggcccgg cagatgacgg cggcgatgga gacgcaaggg atacccgtcg ccaccgtagg
    11161 catcgccacg ccgcacagcc gccagggcca cgactacgag accgacgccg tcgcgccgac
    11221 gtacccggtg aacctgatct gcgtcaacgc cgacgccctg ccggacttcg cggcccacgc
    11281 cggcctagac ctcttcaaga accgctactc ggtggggatg tggtggtggg aggttagcga
    11341 gttccccgag cgtttcgaca aagcgttcga gtacgtggac gaggtgtggg tcggctcgca
    11401 ccacgtcgcc gacgcggtgg cgaaggtctc gccggtcccg gtggtgaagg ttacagtccc
    11461 gttctcgatg ccgccggtcc cggagatgag ccgggaggag ctcgggctgc cggagggctt
    11521 cctgttcctg ttcagcttcg actaccacag cgtcttcgag cgcaagaacc cgctcgcggt
    11581 ggtcgaggcg ttcaaaaagg cgttcgcgcc gggttcgggg gcgtcgctcg cgatcaagtc
    11641 aataaacggc gacaagcacc ccgaggagca cgcgcgcctc gtggccgccg ccgaggacca
    11701 cccggatatc cgcgtcatag accgttacgt ctccttcgcg gagaagaacg cgatagcggg
    11761 ggcgtgcgac tgctacgtct cgctgcaccg ctcggagggc ttcgggttca cgatggccga
    11821 gcccatgtac ctgggcaagc ccgtgatcgc caccgcctac tccggcaaca tggacttcat
    11881 gaccgaccgc aactcctacc tagtggacta cgacatcgtg cccatcggcg agggagcgga
    11941 cccctacccg ccgaccggcg agtgggccga gcccgacgtg gaacacgccg cgagcctgat
    12001 gcgccgcgtc ttcaagaacc ccgaggaggc ccaagagcgc ggcaagcgcg ccgccaggga
    12061 cataaggcgc acccaatccc cggaggccgc gggcaaagcc atagccgcgc gcctctccca
    12121 catccgggcg cgtttggggg cgggcgccgg ggagcaaacg gcgcgcacgt ccgcggcgca
    12181 gcagcatccg gggctcaaaa cgggggcgct ggagaacagg atcatgcaca cccccacgct
    12241 gccggtgacg ccctcgctgc cggggagggt catgggcttc gcccggcggg tggccctcaa
    12301 gctcaactgg ccgttcgcca tgcagcagcg gaccatcaac gaccagctac tcaccatgat
    12361 caagggcgtg gacgccaccc tgcgcgacgt tactggccgg ctagagaagc agggctcccg
    12421 gacggtagcg gggctcgaag cgcagaccaa agagattgaa gcgctctacc cgcgcgtcga
    12481 ggccgccgac aggctcatcg ccgaaactcg ggcgctcccg tacatggcga actccccgtt
    12541 cgagacgttc gaggagcctg aggccggcag cgtcttcggc tacgaggaac gcaacggctc
    12601 gtccgggagc gcggggacct acgacgcctt cgaagacatc ttccggggcg acgaggcgtt
    12661 tatccgcgag aggcagcgcc gctacctgga catcctcggg ggccgcgagc cggtgctgga
    12721 cgtgggctgc gggcgcggcg agttcctgga cctgttgcgc gaggaaggca tcccctacac
    12781 tggcgtagac ctcgatcccg acatggtcga gcgctgccgc aggaagggcc acgagaacgt
    12841 cgaggtcgcc gacgcgaact cctacctgga gaagcgattg gacgactcca tcggcgtgat
    12901 cttctgcgcc caggtcatag agcacctgcc ctacgaggaa ctgctgcgct tcttcgagct
    12961 tgccgagagg aagctcgcgc ccggcgggct cttcgtcgcg gagaccgtca acccgcactc
    13021 cgtcccggcc ctcaaaacct tctgggtgga cctcacgcac cagcaccccg tcttccccga
    13081 ggtaacgctg gccctgtgca agataaacgg cttcgacaag gcatacgtct tccacccgaa
    13141 cggcgcgggc gacgtggaga aggaccgcta cgagaccggc gagtacgccg tggtggcgac
    13201 gaaggcggag cggccgtgag cgccggaggc gacgccagga gaaacggccg ggacttcgcg
    13261 gaccggctcc cgaaggggct gcggggactg gtccgtttgg cgacggtgaa cccgcgcctg
    13321 gaccgcgtcg tcgcgaacca gaacaggctc ggagagcagg tgaagcggat cgacgcccgc
    13381 tcgcaacagc tcaccatcca gactcgggag ctggaccaga agctgcagaa cgcgaacctc
    13441 gccgggatgc gcgagaccat aaactcccag aaggcccgca tagaccagct agaggcgcag
    13501 ctctccgaga tgctcgcggg caacgcgccg gtcgggaagc gggagttgtc cgagcgggag
    13561 ctgcgggcgg agatctcgaa ggtagacaac tggtaccaca ccatccagtt agcccccggc
    13621 cacttcaccc ccggctctca caagtcggac ctggcgctcg ccgcgatgca cgtgcccgaa
    13681 gacctctccg gcaagcgcgt gctggacgtg ggagccaggg acggattctt ctccttcgtc
    13741 gccgaggcgc ggggcgcgga agtcctcgcc atagacgccg tagcccccaa cctcaccggc
    13801 ttctccgtcg ccgcgccgct catggactcc tccgtccggt tcgagaccat gaacgtctac
    13861 gacctggacc cggaaacggt aggagaattc gacgccatct tcttcctggg cgtcctctac
    13921 cacctcagag acccgatgct ggcgctggac aggctctggt ccgtggcgaa gcccggggct
    13981 acagtctggg tcgagtcgca cgtcgcggac tacgggcttg ttgacccgga gagcggcgag
    14041 tggcggagcc tcgcggcggt cgcgccggac ctcgtggacg tgccgatggc gcagttctac
    14101 ccggacgacc tgctggcgaa cagcaccacc aactggtggg ggccgaacct ggccggcctg
    14161 cgggcgatgg tcgaggcggc ggggttcgag atcctgcgca cccgcttcgt cggcgggcgc
    14221 ggcctcgtcg tgggccgcaa ggtcgaggac gaggacaccg acttctaccg cgagttcgac
    14281 cgcggtaccg ccactggcga ggaggggatg gcctgggaga gcgtcaagca atggtacaac
    14341 gcccggccgc ccgccgagag caagaccgcg ctcaggcgca acagagaagt ctcgcccgag
    14401 gaggcgcggg ccgaggtcgc gaagttcgag aactggtacc accgcatcga ggtagccccc
    14461 ggcgtcgtga ctcccggcgt ccacgactcg gaggccgcac tggaggcgat ggaggtcccc
    14521 gagagcttcg agggtatgcg cgtgctggac gtgggggctc gcgacggcta cttctctttc
    14581 gtcgccgagg cgcgcggcgc ggaggtcctc gccattgacg ccgtcgcctt agagcacctc
    14641 cagggcttct cgacggcagc cgcgctgttg ggctcgtcgg tggagtacaa gaccatgaac
    14701 gtctacgacg tctcgcccga gaacgtgggg cagttcgacg ccatcttctt cctgggcgtt
    14761 ctctaccacc tgcgcgatcc catgctggcg ttggacaggt tgtggtcggt ttgcaggccc
    14821 ggagcgacca tatgggtcga gtcccacacc atagatcacg ggttcataga cccggcgacc
    14881 ggcgagcaga agtctttcgc ggcggtggcc ccggcgctgg cgaagtatcc gatggcccag
    14941 ttctacccga agggtctgtt gggggggaac cagacgaact ggtgggggcc gaacctggcg
    15001 gggctggagg ctatggtgcg ctcggcgggc ttcgaggtgc agaggagcag gctcgtcggt
    15061 ggccgaggac tggtcgtggg gaagaaggtc gaggacgaaa acaccaactt ctatcgctgg
    15121 tacgatcgcg gcgtcgtaac ggacaagaaa gaccagcggg cgtaggagcg gtagaaagga
    15181 gtctgcggac aaacatgggc atcttgagaa aactttggca gcgcgaggac aaggaccagg
    15241 agataccgat cccgccgctg gagatgcgcc agctcgtcgg gccgacggac ccggcggcct
    15301 tcgataaccc gaccggggag ctggtcttcc cgtttttgcc ggagtccgcc ttcgaggcgg
    15361 tattcgacta cgggtgcggc tgcggccggc tcgcgcgcca gctcttgcag cagaagcccc
    15421 gcccgaagcg ctacgtcggc gtagatatcc accgcggcat gatcgagtgg gaccagaaca
    15481 acctgacgcc cgtggacagg aacttccagt tccaccacca cgacgtctac aacccgagcc
    15541 atgcccccac caactccagg cggctcaccg cacccttcga ggtcgaggac aaggggttct
    15601 cgctggtgat cgcgtggtcg gtcttcaccc acatctacga ggcgcaggcg ctccactacc
    15661 tgaaggagat agcccgcatc atgcgccccg acggggtctt cctctcgacg tggttcctct
    15721 tcgacaagcg cgaattcccg atgatgcagc gcttccagaa cactttgttc gtcaacgaga
    15781 ccgacccgac gaacgccgtg atattcgacc gagagtgggt gaaggaggcg atctcccagg
    15841 ccgggctctc catcgaggag gccgtgccgc cgagcatccg cggctaccac tggcgcgtta
    15901 ccatgcgcca caagcagccc ggcgacggac ccttcgagtt cccaccggac gaggccccct
    15961 acggctcgat gccgccgccc ctcatgcccc agaccacgcc gcccggcgac ggccagggcg
    16021 gccagggccg cggcgggggc tgatgccggc cttcaagcgc cgcctttcgt tgaagctccg
    16081 gcgcaacctg cgcctctccc tcgacgtgga gggtgagctg cccgacctcc gcaacgtcgc
    16141 caaccgtctc aagggcggga agcccgcccc ggcatccggg acggcgcggc gcggcgcgga
    16201 accccggccg actggcccga tgcccacccc gaacacgcag ctatcgtcag aggatgtgag
    16261 ggtggagatc tcgaaggtag agcactggta ccaccagatc gaggtctcgc ccggcatcac
    16321 cacgccgggc gtcaacccct cggccaatca gctcgccgcc atggaggtcc cggccgatct
    16381 ctcggggatg cgcgtgcttg acgtgggggc gcgcgacggc tacttctcct tcgtcgccga
    16441 agcccgaggg gcggaggtcc tcgccataga cgccgtggcc ccaaacctca ccggcttcga
    16501 cacggcggcg ggtctcctgg gctcgtcggt ggagtaccgc acgatgaacg tctacgacgt
    16561 ctcgcccgaa gaggtagggc ggttcgacgc ggtgttcttc ctcggggtcc tctaccatct
    16621 cagagacccc atgctcgccc tggacaggct ctggtccgtg gcgaagcccg gagcgacgat
    16681 ctgggtggag tcccacacca tagacagggg tttcgtcgac ccggagacga aagaggtccg
    16741 ggatctctcg gaggtcgccc ctaacctcgc cggggtcccg atggcgcagt tctaccccgg
    16801 cgaccagctc ggtgggagcg tcgccaactg gtgggggccg aaccttgcgg gactggaggc
    16861 gatggtgcgc tcggcgggct tcgaggtgca gaggagcagg ctcgtcggtg gccgaggact
//
LOCUS       DASSMB010000028         5353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_893978, whole genome shotgun sequence.
ACCESSION   DASSMB010000028 DASSMB010000000
VERSION     DASSMB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893978"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1486)
                     /locus_tag="VFJ72_02050"
     CDS             complement(17..1486)
                     /locus_tag="VFJ72_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HET7478277.1"
                     /translation="METSKPSRQRDTRDQRHEGIASKVEGFNERAARAVRDEDLHASI
                     ALFTNKTLTGRGATLGALPEAPELRERAYHIKQETMANLDRYLEQMADAVEARGGNVF
                     FARDGGDVARYIGELARRRGAKVITKSKSMATEEIELNPRLEEDYADLGLEIVETDLG
                     EWIAQLAGEHPSHIIGPILHMNRRRVAQILSETAGEELPPDVEALGSFARKRLREKFL
                     TADIGITGANFGVAETGTVVTVTNEGNARLVTSVPPVHVVVMGIEKLVPRLADLSVFV
                     RLLARSGTGQKITVYTNFITGPRGEGELDGAGEYHLILMDNGRSKLLGTEFEEALYCI
                     RCGSCLNVCPVYRQTGGHAYGSTYSGPIGAVITPLLKGDREAKDLPHASSLCGACSET
                     CPVGIPLHELLLKLRNRQVEEGIAGRAQSLMFKGFEKTMKSPRLYGIGTKAARISQRP
                     AVKDGSVRPMPGPMKGWTDSREMPPLAPRSFRELWKEGI"
     gene            complement(1489..2241)
                     /locus_tag="VFJ72_02055"
     CDS             complement(1489..2241)
                     /locus_tag="VFJ72_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET7478278.1"
                     /translation="MRVALFIPCYVDQINPEVGVSVVSVLRRLGVEVTYPEGQTCCGQ
                     PAFNSGFFDEARGVARHFLDVFERESERESFDYIVCPSGSCTAMAAHYYPFLFKELPE
                     DRERSEALGHRVRELSDFLVNVMGVSDLGARYEGSAVFHTGCHQRRELGVLDEPRKLL
                     GNVEGLEMREWENEELCCGFGGTFAVKMPDVSTAMADEKIAALDKSGADTLVSCDSSC
                     LMHLGGRLKRTGHDTRVLHLAQVLDSGNGRWQ"
     gene            complement(2359..3114)
                     /locus_tag="VFJ72_02060"
     CDS             complement(2359..3114)
                     /locus_tag="VFJ72_02060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET7478279.1"
                     /translation="MAFSGEVAWVTGSSTGIGRAAALELARQGCRVVVHYNSSESEAR
                     KVLEEIEGSGGEAMLVGGNVSDAAEVKRMAGEIEARFGRVDILVNNAGSLIERRPFAE
                     MTEDLWERVMDVNLKSVYLVSQAVLPMMQRQGGGRIVNVTSVAARNGGSSGSTAYASA
                     KAGVSTLTRSMAKELISENILVNAVSPGVIATPFHDRFTEPEAREKRAKTIPIGREGK
                     PEEVAGVVAFLASPAADYLVGEIIEINGGQLMG"
     gene            3193..3678
                     /locus_tag="VFJ72_02065"
     CDS             3193..3678
                     /locus_tag="VFJ72_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478280.1"
                     /translation="MRAVGYVDLRSRDRGERTRDRRKRAGLWRGIRTNPSILIAAGFA
                     FGVAASSTVLIPASGGYEPLVLIVILIFLLLVSVGIWQVLRHEPEDARPKLGGEKQLL
                     MAMRKAGGSITPVEAALETSLTVDEAEEVLSRFAGQGYLRVESVDGNLYYTLPGRHPG
                     A"
     gene            complement(3732..4610)
                     /locus_tag="VFJ72_02070"
     CDS             complement(3732..4610)
                     /locus_tag="VFJ72_02070"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7478281.1"
                     /translation="MKRLGFIGLGAMGSPMAACLVREGFDLSVFDVRPESAAPLVEMG
                     VTGAASPREAAEGVDALFLMVVNAEQAGEALFGENGAAEALKPGAAVVLMSTVGPGPV
                     RELGERLAEIGARVLDAPVSGGVARAETGDLLIMAGGPPELFEELRPALAAVGSTVVH
                     CGDSVGDGQSVKLVNQLLCGVHIAAAGEALAYAAALGLDPRSVFETIRHGAAASFMLD
                     DRGERMLERRFIPAKSALDIFVKDMGLVREAAGERGFQTPLADAAHRLYETGSSLGLG
                     REDDSGILRVFERGTD"
     gene            complement(4611..>5353)
                     /locus_tag="VFJ72_02075"
     CDS             complement(4611..>5353)
                     /locus_tag="VFJ72_02075"
                     /inference="COORDINATES: protein motif:HMM:NF028352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HET7478282.1"
                     /translation="RGVGGRPVVVNAASYADLEVFVLGLLSAEESGKRFIYRTGPSFV
                     RTRGGISERGPLSAEELYRRRPRRGHGLVLVGSHVAMTTRQLERALALDGVRAVELSV
                     PKLLEEAEWQGELDRAVEEVNRGLAEAEVIVYTSREVISGGAGRSGFEVGRSVSEALV
                     EVMRRVDPESPLGFVVAKGGITSSDIGTKGLGVRRAEVAGQMLPGIISVWILPEDSPF
                     PGLPYVIFAGNVGEPDSLAKVIQILREG"
BASE COUNT          848 a         1969 c         1633 g          903 t
ORIGIN      
        1 tccgtgtgat cggacactag atcccctcct tccacaactc gcggaaggac ctgggggcga
       61 gcggcggcat ctcccgcgag tccgtccagc ccttcatcgg accgggcatc ggcctgaccg
      121 aaccgtcttt caccgcgggc ctctgggaga tcctggccgc cttcgtgccg atcccgtaca
      181 gccttgggct cttcatcgtc ttctcgaagc ccttgaacat cagactctgc gcccggcccg
      241 cgatgccttc ctctacctgc cggttcctca gcttcaggag aagctcgtgc agcgggatgc
      301 cgaccgggca cgtctccgag cacgcgccgc acagcgagga ggcgtgcggc aaatccttcg
      361 cctccctgtc gccctttagc aagggtgtga tgaccgcgcc gatgggaccg gagtaggtcg
      421 agccgtaagc gtggccgccg gtctggcgat agaccgggca gacgttcagg cacgagccgc
      481 accggatgca gtagagcgcc tcctcgaact cggtgcccag gagcttcgag cggccgttgt
      541 ccatgaggat gaggtggtac tctccggctc cgtccagctc tccctcgccg cgcggtccgg
      601 tgatgaagtt ggtgtagacg gtgatcttct gccccgtccc gctgcgcgcc agaagccgca
      661 cgaacacgga gaggtccgcg aggcgcggca ccagcttctc tatccccatc accacgacgt
      721 gtaccggcgg cacgctcgtc acgagccggg cgttgccctc gttggtgacg gtcacgaccg
      781 tgccggtctc ggccaccccg aagttggctc cggttatgcc gatgtcggcg gtaaggaact
      841 tctcgcgcag gcgcttgcgg gcgaaggagc cgagcgcctc gacatccggc ggcagttcct
      901 cgcccgccgt ctccgagagt atctgcgcaa cgcgccggcg gttcatgtgc aggatcgggc
      961 cgatgatatg cgagggatgc tccccggcga gctgggcgat ccactctccc agatccgtct
     1021 ccacgatctc cagcccgagg tcggcgtagt cttcctccag gcgggggttc agctcgatct
     1081 cctcggtcgc catgctcttt gacttggtga tgaccttcgc cccgcgcctc ctcgccagct
     1141 ccccgatgta ccgcgcgacg tccccgccat ccctggcgaa gaagacgttg ccgccgcggg
     1201 cttccacggc gtccgccatc tgctccaggt agcggtcgag gttcgccatc gtctcctgct
     1261 ttatgtgata cgcccgctcc cgcaattccg gcgcctccgg tagcgccccg agagtcgcgc
     1321 cgcgcccggt aagggtcttg ttcgtaaaca gcgcgatgga ggcatgcagg tcctcgtccc
     1381 gcacggccct cgccgcgcgc tcgttgaacc cctctacctt gcttgctatc ccctcgtgcc
     1441 gctggtccct cgtatctctc tggcgggacg gcttgctggt ctccatagct actgccacct
     1501 cccgttcccg gagtcgagta cctgcgccag gtgcaggacg cgggtatcgt gccccgtgcg
     1561 cttcaggcgt ccgccgaggt gcatcaggca gctagagtcg cacgaaacga gcgtgtccgc
     1621 gccgctctta tcgagggcgg ctatcttctc gtccgccatc gccgtcgaga cgtccggcat
     1681 cttgacggcg aacgtccccc cgaacccgca gcacagctcc tcgttctccc actcgcgcat
     1741 ctccagacct tcgacattgc caagaagctt ccgcggctcg tccagcacgc cgagctcccg
     1801 ccgctggtgg catcccgtgt gaaagacggc cgatccctcg tagcgggcgc cgaggtcgct
     1861 gacgcccatc acgttcacca ggaagtccga taactcccgg acgcggtgtc ccagcgcctc
     1921 ggagcgctcg cggtcctcgg gcagctcctt gaagaggaac ggatagtagt gcgcggccat
     1981 cgcggtgcac gagccggagg gacagacgat atagtcgaag ctctcgcgct cgctctcgcg
     2041 ctcgaagacg tcgaggaagt ggcgggcgac gccacgggcc tcgtcgaaga acccggagtt
     2101 gaaggccggc tggccgcagc aggtctgacc ttccgggtag gtaacttcaa cgccgagccg
     2161 gcgcagcacg ctcaccacgc tcacgcctac ttcggggttg atctggtcca cgtaacacgg
     2221 gataaatagg gccaccctca cgactcgcaa tcctcccacc atcgagttcc agagccagta
     2281 taacaacgtg gtcggacccg ccgctgcggt gaaaatcgga ccatgacgcg cgtccgatgc
     2341 ggcctctcga tgatggatct aacccatcaa ctgcccgccg ttgatctcga tgatctcacc
     2401 gaccaaataa tccgccgcgg gtgaagcgag gaatgcgacg actccagcga cctcttccgg
     2461 tttaccctcg cgcccgatgg ggatggtctt cgcccgcttc tcgcgggcct cgggttccgt
     2521 gaagcggtcg tggaaaggcg tggctatgac gccgggagag acggcgttca ccaggatgtt
     2581 ctcggagatt agctccttcg ccatcgagcg ggtgagggtg ctgacgccgg ccttggcgct
     2641 cgcgtaggca gtcgagccgg agctgccgcc gttgcgggcg gcgacggagg tgacgttgac
     2701 gatcctacct cccccctgcc gctgcatcat cggcaagacg gcctgcgaga cgaggtagac
     2761 gctcttgagg ttcacgtcca tcacccgctc ccacaggtcc tcggtcatct cggcgaacgg
     2821 gcggcgctct atcagggagc ccgcgttgtt taccaggatg tccacccgcc cgaagcgggc
     2881 ctctatctcc cctgccatcc gcttcacctc agcggcgtcg gagacgttgc ccccgaccag
     2941 catcgcctcg ccgcccgagc cctcgatctc ctccagcacc ttccgcgcct cggactcgct
     3001 gctgttgtaa tggacgacga ccctgcaacc ctgcctcgca agctccagcg ccgccgcgcg
     3061 cccgatcccc gtgctgctcc ccgtgaccca cgcgacctct cccgaaaacg ccatctcttg
     3121 ttgcctccct gtaatctagc ctcgttacaa ttgatgcttc atagcacaaa ttgtcgggac
     3181 gggcaaagga gcgtgagagc cgtgggctac gtggacctgc gaagccggga tcggggagag
     3241 cgcacgcggg accggcggaa gcgtgccggg ctctggcgcg gtatacgaac caacccctct
     3301 atactgatag ctgcgggttt cgcgttcgga gtcgccgctt caagtaccgt tctcataccc
     3361 gcgtccggcg gatacgagcc tctcgtgctc atcgtgatcc tgatcttcct gctcctggta
     3421 agcgtgggta tctggcaagt gcttcggcat gaaccggagg acgcgcgtcc gaagctcggt
     3481 ggtgagaagc agttgctcat ggcgatgcgg aaggcgggcg gcagcatcac ccccgtcgag
     3541 gcggcgctgg agacctcgct aacggtggac gaggccgagg aggtcctctc caggttcgcc
     3601 ggccagggct acctgcgggt agagagcgtg gacggcaacc tctactacac cctgcccggc
     3661 cgacaccccg gcgcgtaggc gttcagggtc tcattcccgg cgcgtgcccc cgtcgtgcag
     3721 ggggtctaca ttcagtccgt gccgcgctcg aagacgcgca ggatgccgga gtcgtcctct
     3781 ctgcccaggc cgagcgagga gccggtctcg tagagccggt gggccgcgtc ggcgagcggc
     3841 gtctggaatc cccgctcccc cgccgcttcc cgcaccagac ccatgtcctt gacgaagatg
     3901 tccagggcgc tcttcgccgg aataaaccgg cgctccagca tccgctcgcc ccggtcgtca
     3961 agcatgaacg aggcggctgc gccgtgccgg atcgtctcga agaccgagcg ggggtcgagg
     4021 cccagagccg ccgcatacgc cagcgcctct cccgccgccg cgatgtggac cccgcacagt
     4081 agctggttca cgagcttcac ggactgcccg tcgcccaccg aatcgccgca atgcacgacc
     4141 gtcgagccca ccgccgcgag cgccgggcga agctcctcga acagctccgg cggtccgccc
     4201 gccattatga gcaggtcccc ggtctcggcc cgcgcgaccc cgccgctgac cggcgcgtcc
     4261 aggacgcgcg ccccgatctc cgccagcctc tcgcccaact cccggaccgg ccccggcccg
     4321 acggtgctca tcaggacgac ggcggcgccc ggcttcaggg cttcggcggc gccgttctcg
     4381 ccgaagagcg cttccccggc ctgctccgcg ttcaccacca tcaggaagag cgcgtccacg
     4441 ccttcggcgg cttcgcgagg cgaggcggcg ccggtgacgc ccatctcgac cagcggcgcg
     4501 gcgctctccg ggcgcacgtc gaagaccgac aggtcgaagc cttcccgcac gaggcacgcc
     4561 gccatcgggg aacccatcgc cccgagacct atgaatccca gacgcttcat ctatccctcc
     4621 ctgagaatct ggatgacctt cgcgagcgag tcgggctcgc cgacatttcc ggcgaagata
     4681 acgtagggga ggccggggaa cgggctgtct tccggcagga tccagacaga tataatgccg
     4741 ggtagcatct gtccggcgac ctccgcccgg cggaccccga gccccttcgt cccgatgtcg
     4801 ctggaggtga tcccaccctt cgccaccacg aacccgagcg gcgactccgg gtccacccgc
     4861 cgcatgacct ccaccagcgc ctccgagacc gagcgcccga cctcgaaccc cgagcgaccc
     4921 gcgccgccgg agataacctc ccggctcgtg tacacgatga cctccgcctc tgcgagcccc
     4981 cggttgacct cctccaccgc ccggtccagc tccccttgcc actccgcctc ctccagcagc
     5041 ttcggcaccg acagctccac cgcccgcaca ccgtccagcg ccagcgcccg ctctagctgt
     5101 cgggtcgtca tcgccacgtg cgacccgacc agcaccagcc cgtgtccccg ccggggacgc
     5161 cgccggtaca actcctccgc gctcaacggt ccccgctccg agatgccgcc gcgcgtccgc
     5221 acgaatgacg gtcccgtgcg gtagataaag cgcttgccgg actcctccgc ggagagcagg
     5281 cccagcacga agacctccag gtcggcgtaa gacgcagcgt tcaccaccac gggccggccg
     5341 ccgacgccgc gca
//
LOCUS       DASSMB010000029        52588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_767120, whole genome shotgun sequence.
ACCESSION   DASSMB010000029 DASSMB010000000
VERSION     DASSMB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 52588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52588
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767120"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFJ72_02080"
     CDS             <1..297
                     /locus_tag="VFJ72_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7478283.1"
                     /translation="SYDSDLSNVERVTVEVANHVMEEIEGGVPEFEPSVRFNNFGDFS
                     IDFSVSMQTREVVEQYRIKHEFIKRLHERYWKEGIEIPSPPVAALPSSGKSQEA"
     gene            420..1142
                     /locus_tag="VFJ72_02085"
     CDS             420..1142
                     /locus_tag="VFJ72_02085"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET7478284.1"
                     /translation="MKNVDSKGSGAEIGSAPDEVAERLREAVAQPLDTWREVYESFSP
                     VDVVTGERMRRIPPPSGGARRAAVLVPVLIEPDGAHIVYTVRRDHLNDHAGQISFPGG
                     GMDAGDESLLDTALREAEEEIDLSRDLVEVVGALEEMYIPPSNFHVSPYVGLLPPEAE
                     LVLAPDEVEEIFTVSLRSLMAPETFRRVLWRRDGREYEVPVFAVETPEPRQIWGATAG
                     MTAALLTRLGWREYRHQTSSIQ"
     gene            1471..1971
                     /locus_tag="VFJ72_02090"
     CDS             1471..1971
                     /locus_tag="VFJ72_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478285.1"
                     /translation="MSFLGLVERGAHGSEFLVTGLLLAGVLFLALTTGWFLVTKRTLP
                     VRDLEPVEIVWYSREAEAVLLEVAPLFMALDERERTIEAYLRSVDASRAREVREAVRL
                     TGVRELWSSFARASALVEDDPLRGVDELRRLRPMLRECVTSLDGLMDRLKLEVAEEKG
                     EKNARS"
     gene            1958..3841
                     /locus_tag="VFJ72_02095"
     CDS             1958..3841
                     /locus_tag="VFJ72_02095"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET7478286.1"
                     /translation="MQGPRDAGLMERLAALEAEVERQGCELRGVMRRLDLLAGKYALA
                     DGPPHVPERTDAQERGFSDVTRTLREKKFRLPLALERLRSGEWWLNRVGIGLLLLGAV
                     FLFKFSVDRDWITPPVRVGFGLALGALLVTLGLRVYAGRRSFSQVLLGGGVGVFYITG
                     YAAFELYALVPYPAAFSFMVMVTLMAFTLALRQNGVALALIGALGGFGTPLLLYDSGG
                     SVSGLVLYITLVLAGACAIYLYRGWRSLLMAAFAGVWTVLLIGSWSLPYPPDAALSDR
                     LALQVGAALAWSILWVSAMGREVLRDRAASRRGSEHSNAPAHAISHAICIAAPLAALA
                     YTREIWDLQGDTLGWISLGAATLYALASTFILRMSGGGLTYYTQAAGALVLATLSLVL
                     LLDGDALLVALAAEGAALHYVARRLPDRLLAVGANALLAIALACIFTRPLWDYPLSGQ
                     IAVFNTRSLSDLAVITLAFAATFSVTAWRAAIILRLAAHAALLAWFWRELSVIPGGEP
                     YVTIAWGLYAAALLVAGLRLDSTALARTGMATLFLVVGKLFLFDLAGVEAVWRILLFL
                     GFGGLFLGLSYYLHSLWRPKALDTTQSPQVTTRNPGGPGAEMKLAQGKRDPTTYGVLK
                     RKL"
     gene            4226..4528
                     /locus_tag="VFJ72_02100"
     CDS             4226..4528
                     /locus_tag="VFJ72_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7478287.1"
                     /translation="MISEERVRDQLRNVIDPEIGMDLVELGLIYDVGVHDEGRHVDVT
                     FSLTSPMCPVGDLIQEQVETETLSIEGVETVNAQLSFDPMWNPEMMSPAAKLFFGR"
     gene            4591..5340
                     /locus_tag="VFJ72_02105"
     CDS             4591..5340
                     /locus_tag="VFJ72_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HET7478288.1"
                     /translation="MNIKGLLTDLDGTLFVGEDPVPGAREAVEEIKSAGIHLRYVTNT
                     TRRPRRAVAEQLRALGFEVEDREIFTPARAAAGLIGGKSCLALVDESLHEDLEGVSLT
                     GEHPEYVLIGDLGKGFTYERLDAAFRRLMDGAELVALQKNRYWQTSDGLSLDAGPFVA
                     ALEYASGKTATVVGKPERGFFALALREMGLEASEAAMIGDDAEADVAGARRAGLTGIQ
                     VRTGKWRPGTEESGADLTLDSFADLPRALGL"
     gene            5422..5823
                     /locus_tag="VFJ72_02110"
     CDS             5422..5823
                     /locus_tag="VFJ72_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HET7478289.1"
                     /translation="MKVTDIPVRTEDRYQLVSITGEVRRAVEEAGVEEGLCVISSTHT
                     TAAITVNEDYDRDVPRDLAAACRAFLNTLDVRFDHAEGNSDSHLLTSLFGQSQTLLVR
                     EGELDLGRWQGVFLAEFDGPRSRTVRVAVTG"
     gene            5933..7336
                     /locus_tag="VFJ72_02115"
     CDS             5933..7336
                     /locus_tag="VFJ72_02115"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7478290.1"
                     /translation="MNGWVKSGLALGGIAVGLGAIRRGLNPRPRYAPWEKPRYEDFEK
                     KILIVGGGFGGYTAATKICQATRKREDVGVLVIARDNFFTFWPMVPGIVSSDIDTRNV
                     AQPLRRALIMAGSSFRRAEVTGVDYDRKVVIANDGIEFPYDQLIISVGGQPNFFGIPG
                     VEEHSLTMKSVEDAERIRNRVIERFEEVSLIRGEVPESKLTFVVIGGGATGTEVASEI
                     HTLVHEALAPDYPNIDPSRVRIFLLEGLPQILPELDPALQRAARARLHSERIDVRVNT
                     FAEEVTANSVRLKGGEEIESENVIWTAGARPNVAIQNLDLPINERTGIKVDKYLRVEG
                     RPGIWSIGDCASIPDIRQENGKVVPPTAQAAIQEGYTVAKNVIKALDGETDFEEFEYR
                     PLGQLVELGSDFAVNEVMGVRFSGLAAALFWRMTYLVRLNSPQSKLRVFADWVIGYML
                     RPAVTQIRGSREETHRP"
     gene            7420..7989
                     /gene="pgsA"
                     /locus_tag="VFJ72_02120"
     CDS             7420..7989
                     /gene="pgsA"
                     /locus_tag="VFJ72_02120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET7478291.1"
                     /translation="MNLANQLTLARVIAVVPVMGAIYFAFPGHRWVVLVLYVLAILTD
                     YFDGIAARRSNKVTGFGKLMDSIADKALIVSLFFALVGIGLMPSWMAAIMVIREFAVT
                     GLRMVALESGEVIAANSWGKAKMNSQSVAVFVLLLGYALEGGTGDLFVAAGWWLMLVA
                     VVLTLISGWSYLKDTPRILSVQSERDQRL"
     gene            8025..8864
                     /locus_tag="VFJ72_02125"
     CDS             8025..8864
                     /locus_tag="VFJ72_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478292.1"
                     /translation="MENEPDRRLSPAEVEMVLRRAAELNARRRPRGSGEGTSISPEVL
                     IQVAAAAGIPEGDVRRAMWDLASEKTAEPHTFARRFYGPARLRAVREVEQPVESAREH
                     LESLLRVEQGLKMRRKTEASSLWDAGDMLGVVRRALDFSGHRTLLKAHSIELRVEEVE
                     EERCGANLTADVSNQRGEYMSLGGILGATLALPLAIAGFQSPLFFLAVIPALVAPGIG
                     FKFAYLRACAEIRRALDELLDAAEEGPPRGQEPRERGDRPPGQIRGLQPIPRFTPQSS
                     DDE"
     gene            8913..11057
                     /locus_tag="VFJ72_02130"
     CDS             8913..11057
                     /locus_tag="VFJ72_02130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HET7478293.1"
                     /translation="MPLDVLREIFGFDSFRPGQEEVIRAVLDGQDTLAVMPTGGGKSL
                     CYQVPALMKESLTVIVSPLISLMKDQIDSLVQSSVTDAATLHSGLSPEERWEVERRVR
                     TGEIKMLYVAPERLRSLEFVLSLRRSGVGLFVVDEAHCISEWGHNFRPDYLFLPRAVR
                     DLGSPPVLALTATATPRVREDIVSSMRLREPKVVVTGFNRPNLTYRVREVRQKKHKLD
                     HILDIIRTSPPPGIVYATTRKECEELTAGLRRAGVEAATYHAGLGSAERSSVQERFMT
                     DEVPVVVATVAFGMGVDKPNVRFVIHASVPGSLPAYIQEAGRAGRDGEPSECVVLYRN
                     EDIGRRKRLISLNAAGGAEVESFFRALVGVEREGRVSVPPGSLAALGGVEPDAAGIML
                     RSLEESGLISRGYDLWAEVEVRRVEEEPDGLRDELAAVHAALPGSGLIGLPDLASRAG
                     LRPAVAQGALYRMMLDGILEAVPRGSLVDVRLKTAALDERIRRTIASRLKSHDSAAYS
                     QVRDVQDYANLSKCRREYLLRHFGDTEEVAPCAGCDVCLGETSRQVTVSGTSGTLDLH
                     ASSGQVESAAGPVDSELLERLKSWRSEQAREQKVPAYVVLHNSHLEEISIRKPRSVHE
                     LGSIKGIGLRRAARYGEELVGLVHGNAETASRPSGNSYREHLEAAERLLRSGRGADAV
                     PELVRALESGAEEARMAVDDMLKHTTEKRDTA"
     gene            11074..11598
                     /locus_tag="VFJ72_02135"
     CDS             11074..11598
                     /locus_tag="VFJ72_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7478294.1"
                     /translation="MKLRGKKIAVLIAEGFEDLEYWVTVMRLREEGAEVVSVGPNLDT
                     VSGKNALEASADVTADGVDAAELDGVVIPGGWAPDKLRRYPEITNIVTTVHERGKTVG
                     IICHGGLVAITAGIVNGVRATGSLGIKDDLENAGATWVDEAAFRDGNLVWGRVVPDIP
                     DFCRELVAALSEQS"
     gene            11668..12150
                     /locus_tag="VFJ72_02140"
     CDS             11668..12150
                     /locus_tag="VFJ72_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478295.1"
                     /translation="MRSVLSVGFGVVLALLLGAALVFGIFAPVLSVIFGLETSRSSAM
                     PAILPLIVFAAGFGFYFGGMLAAYYARERRALHGVMVAVTAFGISPVINLASGKGPFP
                     EVRTAPTVTLLLALLAVSVGAAFVGARRGAALYAYNLSYLRKRKAAERRSEEREERKP
                     "
     gene            complement(12158..14038)
                     /locus_tag="VFJ72_02145"
     CDS             complement(12158..14038)
                     /locus_tag="VFJ72_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478296.1"
                     /translation="MSEEPREESNEEVREDIRDPVGEIASQMAAAGSLIDSLEEEVVN
                     LRRDLEEASIALRAAQEEVSARGGALEEADRVRERMDDEISDLRRELNDLRIANSDEQ
                     LRLRNDHINAMAAQRQELDEQRRVEVEAATSEERFITLKEEIRREREASEERHKAELE
                     ALKTSAREWEEKLREEYRDQEERHQRELDELREQAAERRRELEQRLTDSFEERLDEER
                     QAASESREQTLQAIRNAAAGRELELQKDYQAVVETQQAEIEALREEIATRTSEAEDRR
                     KEELRDIKRLAEAREQDLRRAHGAKLAEVKEAGERRLSALQAQREADNRALRARHEED
                     LARVRREYEERLAAEDERRKSETWALEERLGGEKVQRASERRTYAARLKELEERRLAQ
                     KAESEADLERIVERFGEEISDLENRITELEDALAESEATRAGLEEDLDEYRARSENGE
                     RPRLDERPGGELAESSGDGRLAELDSQRVLAEERAQALEEQLREAREESRLNAEELER
                     TRQSLERLSDPSHRLRSGLALFNASEHTRTVASISRALGLPRVHAGIEDEPAGKPVLT
                     FVWGDMAWRRYVSDPTEGVDEPRVYLIGAGDEPSEVQHPGREPNARMDAEGRLTLGIR
                     AR"
     gene            complement(14192..14614)
                     /locus_tag="VFJ72_02150"
     CDS             complement(14192..14614)
                     /locus_tag="VFJ72_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET7478297.1"
                     /translation="MMDGEIRDKFRKSGYTLTSQRRAVLEALEASSGHPSAEDVYLIV
                     KRKNPRVALGTVYQALSVLEEIGVIGSKHWNESPTRYDLNVEPHLDIRCGHCGEVAEI
                     PGVSLDGLENQIKENTSYEVTSATLVVEGVCPRCRRER"
     gene            14708..16390
                     /gene="sppA"
                     /locus_tag="VFJ72_02155"
     CDS             14708..16390
                     /gene="sppA"
                     /locus_tag="VFJ72_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7478298.1"
                     /translation="MLSYILDVLVNSWRFVRNVLAGLRRPPEYIAMEVGGSLPEFEQP
                     VSFLRRRFFPVPTPISIERLRGRLDTISEDGRTTGILLRVRNLDASWAALEEARSELA
                     RFRERGGRVVVYLTDPDTRAYYLACAADEIYATPISTVNIVGLRTRVNFLRDALKQVG
                     VEAEVVAVSPYKSAGDTFVRNDFSRESREQTERLLDRRFAELVSAVAGGRKMTPDEAR
                     ERIDSAPHTAPEAVSQKLLDAACYEDELYDRLGAGDERANIEEWGAARKVLRVPYRER
                     ARKVVGLVRLSGAIVRGRSRRLPVPVPFLGGEQAGDESVVAALRAAEKSRRVAAIIFH
                     VDSRGGDALASDLIWREIERIRAKKPVVVLMGEAAASGGYYVSAAASHIVARSNTITG
                     SIGVIIIRPTTADLYGKLGVNPVAVERGEHAGFLDPSYRPTPEEISSLTGQLRFIYGE
                     FKDRVGKGREMSVEDVEDIAGGRVWTGGEALERGLVDEIGGYRSALRRARELAEIGDD
                     VPESVVRISPPRNVRPAPGEPAPAAIEALKQTFDLTRTTRIWAISPYETNGD"
     gene            16472..17614
                     /locus_tag="VFJ72_02160"
     CDS             16472..17614
                     /locus_tag="VFJ72_02160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478299.1"
                     /translation="MATLSAGHLFTDLNQGAVAALIPFLVAERGLSLAAAGSLVLAAT
                     VSSSVVQPLFGIFSDNRPIPALMPLGVLLGGTGMALAGVAPTFLLILLCVTLSGLGVA
                     AFHPEAARFANYVSGPRRAQGMSFFSVGGNAGFALGPLVTTPLVLLFGLTGTLFLVIP
                     GLLMAVVLLVEIPRMRRFQPAVAESRSDERAAKKLPEHWGPFVRMIGVVTARSFAYFG
                     LVAYVASYYEKVLGVSAALGNAALTVMLASGAVGTLLMGPLADRYGRRAVLAVSMAVL
                     PPLIFVFTLLGPLAGMVDLVLVGAATVGTFGVTVVMGQEYLPGRIGLAAGITMGLSIG
                     LGGLGAPLLGAIADARGLHFTVLVIAALPLLGLLLSLTLPRQHPSV"
     gene            17707..20160
                     /locus_tag="VFJ72_02165"
     CDS             17707..20160
                     /locus_tag="VFJ72_02165"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7478300.1"
                     /translation="MRAFERLGVFVYRYRWPVLVFWAALLLASAFFAPRLGGELKGGG
                     FDGANSEAERVQDLMVDEFGVSPATLTVVFDGRGLPARSREFREKERRALKNLRSLDD
                     VRYVTTYEDTKDERFISKDGKKSYALVGFDISIDQTQDIVDEVRNKVRHDELKTYVTG
                     APAVYLDIQTASNEDIRRAEKYAFPLALVILVVAFGTLVAASIPVVIGGVSVAASLAA
                     LYFVAGSYDMSVFVLSIATMLGLGLGIDYALFAVSRFREELEDFPVPEAVPRMVATAG
                     RSIFFSGTAVLIGLSGLLFFPYMFMRSIGVAGVIVVFVSVSAALTLLPALLGVIGARI
                     NALAIRRGSTAPGESFWGRSAEVVMRHPFAVILLVGAILAALLYPVGHMKIGIPEASV
                     LPMKYESRAGDDVLKKDFDYASLTPMEIVATTPGDPLGAKSLADAKELGKRVEATGGV
                     SSVESVYTVGEQGAKDYAKRITEAREKGRAEAARQVDEGVKKELDKLRDQFGVVPPGA
                     EERVRAEAERRADEKLRRELPKLPEGISASGKVTPEGVANFLALPDVRDRKEIQDARD
                     AYVAKDRTLLRAVTKENPYTEEARAAIEEVRSVDPPPGMSFLVGGLSAGQRDFISSVY
                     GKAPFAAAFVLGVTYLILFLTFRSVFIPLKAVVVNILSLTASFGAMVFVFQDGNLSGL
                     LDFTPLGFVDATLPILMFCTIFGVSMDYEVFLLSRIREAYENGDSNTASVAKGLVATA
                     GIITSAAAIIIVVTGAFSFTGIIITKAVGLGLAVAVFVDATIIRVLLVPATMRILGDW
                     NWWPGGRKRTFRAGPKDSS"
     gene            20253..20672
                     /locus_tag="VFJ72_02170"
     CDS             20253..20672
                     /locus_tag="VFJ72_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7478301.1"
                     /translation="MEFEQVLRSRGYRLTNQRRVIVRELEGERHLSAEEIYDRVKGEH
                     PELGLSTVYRTLDLLHELGIVRKEDFGEGYSRYELSTERMHHHARCRECGVVMEFNEE
                     LMEYLRLQVERETGFVTEWHEITLHGRCAECARANPS"
     gene            complement(20669..21427)
                     /locus_tag="VFJ72_02175"
     CDS             complement(20669..21427)
                     /locus_tag="VFJ72_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7478302.1"
                     /translation="MRVTVVGTGTAAPRLERRQSCVVVETGGELLVFDLGSGAVRGIV
                     RAGLDPLATDRVFFTHFHPDHTVDVVPLLFAMKYGSDEPRTRTLRIAGPQPFVDFWRS
                     ITRVWGEWMIGDYPTEVSELPHACDSPLDLPGCHLSWAPARHRPESISYRLDGDDGTF
                     VYTGDTEYAESVVELARGADTLLIECSFPDESPVPGHLTPSAVARIASEAGVQRVVLT
                     HIYPAADELDLAAEVSHGYGGEVILAEDGFRFNV"
     gene            complement(21429..22040)
                     /locus_tag="VFJ72_02180"
     CDS             complement(21429..22040)
                     /locus_tag="VFJ72_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7478303.1"
                     /translation="MKTTTSTGLWTSITVFALFSAVAGAGWTYSADLALVHAAQRHPT
                     VWLDRLWGYLSVAGGWELTSLALICVAAAMILRGHRRLGTRLIAAFLATALIEYLLKM
                     LLPVHPIPESLGRSEDFAPLVAISYPYPYPSGHLLRSTILLGSLCLWSGSRAVTSLVV
                     IYLACIGYSRFYLGVHWPSDVIGGVLLGIVGLAWTFRSKKGAA"
     gene            complement(22126..23004)
                     /locus_tag="VFJ72_02185"
     CDS             complement(22126..23004)
                     /locus_tag="VFJ72_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET7478304.1"
                     /translation="MSAEKKLSGARADRENETPELLAKYLSHIGQGNLLTHGEEIDLS
                     QRAKAGDKRARQRLIEKNLRLVVSVAKKYRGYGLPFEDLIQEGNIGLMKAVEKFDPDR
                     GFRFSTYATWWIRQAVQRAVADKGRTIRVPVHMTEKIRKVSRAYNELSVEFEREPSEE
                     EVARRLEWEVDEVRLTMSAMPDATSLDQPISTEDTASQLGDFIEDERVSDTPDTVLKE
                     METVQLKEAIERLPDRARYVLVRRYGLDDREPATLAELGDELEISRERVRQLQREAER
                     ILKSGEYGRVLRDAVA"
     gene            23216..24142
                     /locus_tag="VFJ72_02190"
     CDS             23216..24142
                     /locus_tag="VFJ72_02190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET7478305.1"
                     /translation="MEKEHQIPALIRDRRDEIVAEWRRRRDGVMDDEAFARLVAGMGR
                     LVDIFADFLTSPDSVETFSKGGAIHAMVGDIASDQHLLGRDAVGVIEDFSALRRTLWL
                     VVEESVDLSARSGGEVADFFVKLMQASDWVTGSALEAYDDIVRREMEEALGEAAGTDL
                     VTGLPEQDLFTRLLLPDAIASHERFSLAVFDVAGFSETVAAGEVKRARKVLRRLADAV
                     REAAGEHATFARFGDDEVCVLLPGQGSERAYDVAERVLDRLAGRKDSFQVDVGIAEYP
                     AHGEEGEALIVQMLRALKMAKRVGGSGIVVAR"
     gene            24156..24467
                     /locus_tag="VFJ72_02195"
     CDS             24156..24467
                     /locus_tag="VFJ72_02195"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7478306.1"
                     /translation="MIAIMNSLPVKEGAADEIVERFSGSGGHVQDFPGFVSMEVMKSE
                     EEDEVLVITRWRDREAFDSWVRSEEFRKAHGRGGAGELLRGHPKMTSYEVAVERAPGA
                     P"
     gene            complement(24488..24628)
                     /locus_tag="VFJ72_02200"
     CDS             complement(24488..24628)
                     /locus_tag="VFJ72_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478307.1"
                     /translation="MSRERFAFGLLIFVLITLFAITFFVLGRGEYEPGASAEPVVAYV
                     LG"
     gene            24784..25509
                     /locus_tag="VFJ72_02205"
     CDS             24784..25509
                     /locus_tag="VFJ72_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7478308.1"
                     /translation="MSAIPGRFEVDIDPASPEYTMLQEQECRDLLDILSELGIATADR
                     IYEPGDAVYREGEYGDALYVLISGVLKLFRPYSGSKEATLRLLKSWDIFGHLAFAGET
                     KQRAYAEAVTRCKVTKVPKVFVERAVRQEPRVAFKLMTLLELRLVQYEELVKCLLPRE
                     TEVRLANLLPILAQKFGEQNGSGEITINLRLTHQDLAAMVASTRESVTKVLNELRNRD
                     VIEVEAGRITLKDSEVLSRLGRG"
     gene            25595..26413
                     /gene="uppP"
                     /locus_tag="VFJ72_02210"
     CDS             25595..26413
                     /gene="uppP"
                     /locus_tag="VFJ72_02210"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HET7478309.1"
                     /translation="MLELFEAIFLGVVQGLTEFLPVSSSGHLLLAQYFLGVDQSRFGL
                     SFDVALHLGTLVAVVSFFWRDLVRMALAFLRSLRHRDMTDQDQRMAYLVIASTVPAAL
                     IGFLLEDFFASAVRSPWVVAFNLVIVGVLFIVAETVGSQRRPAYKLTFREALGIGFAQ
                     AAALVPGVSRSGATMTLGLFLGLRREEAARFSFLMSVPIIAGAGGLKLGEVIAGGMDV
                     FETLLFGVGFVTSAIVGYLAIRFFIRYIAGHSLRAFAYYRFALAAVVVLLLLLF"
     gene            26667..27776
                     /gene="pheA"
                     /locus_tag="VFJ72_02215"
     CDS             26667..27776
                     /gene="pheA"
                     /locus_tag="VFJ72_02215"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7478310.1"
                     /translation="MFAEKASGNPEEMNEIRAGIDEVDAEIVRLLDRRARLARRIGEI
                     KQARGLDTYAPARERMVLDRVQSLSEGDFPRGGLEAVFREIISSSISLEARLKVAYLG
                     PEATFTHEAARRSFGSSVELEPQTTVSEVFARVERGEAEHGVVPVENSMEGAVTHTLD
                     ELMNSPLKICGEVYLPISQNLLSGAASLDEVAAVVSHPMALAQAATWLRRELPNATTE
                     EVESTAEAARRAAEEPGLAAVGSVLAAEAHGLNILARNIQDARANATRFIVLGRAWAG
                     RTGRDKTSVVFSVKDRPGVLKDALSAFAEKGINLTRIESRPSRKRAWTYVFFADFQGH
                     PDEERVQQALAGLEEHCPYVSLIGAYPEVSPEVVG"
     gene            27788..28012
                     /locus_tag="VFJ72_02220"
     CDS             27788..28012
                     /locus_tag="VFJ72_02220"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7478311.1"
                     /translation="MSFVSAAREVLQEVGYPLHYSDITEIALESGYLRSSGRTPHNTM
                     RARLSVDVRDNPESPFIQTAPGVYSLKRGD"
     gene            28108..29097
                     /locus_tag="VFJ72_02225"
     CDS             28108..29097
                     /locus_tag="VFJ72_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478312.1"
                     /translation="MVELAERAVDAAYKIKNLEDYDRRLRRAARRVDETSRGHGTAAL
                     VIYEAALMSSGRTIDEVRELVDEFHRTWEDEEEPLTIPRVSGIMNVHGDEWFFGDSGL
                     RRLAGRLLGQFQHRVAQYNYVDEREVLKRWADSYDTRLFIRRRILGTEPVVGVVGGLD
                     LQRVQYLRVIAGGNTLVPSQGVARALEALGFETNDDYEILGHGETLALHMDLPAPVVG
                     ALLEDLARDDVRDFPAPPRSAEEPGEEGPGEEGPGEDTGTQKPDAEQRAARRATAGDP
                     GKGEEPTRVQDPQARDTPGVPEESGEASRPASPRSGKGAEPGTSVAPEDKDAE"
     gene            29774..29980
                     /locus_tag="VFJ72_02230"
     CDS             29774..29980
                     /locus_tag="VFJ72_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002832300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET7478313.1"
                     /translation="MPEEQGRVKWFDPDKGYGFLVRPTGEDLFVHHSEVQGDPAALAP
                     GSDVEYEVGENDRGPNARRVRLVD"
     gene            29961..31418
                     /locus_tag="VFJ72_02235"
     CDS             29961..31418
                     /locus_tag="VFJ72_02235"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET7478314.1"
                     /translation="MSGSWTDAPDSSASRFLAAAAIGAGTFFVYLSTLAPTIATNDAG
                     RFQIAAPTLGTGHPTGYPTFILLGKLFTYLPLGDIAYRMNLMAAVFGAAASVLFFLAS
                     REIGGRPLAAAGGALLFAFSSTFWSQATVAEVYTMHATFVLGVLYLLLRWRRSGAAAS
                     LLGAALLYGVSLGNNAGMVLLAPAFAVLVLAGRWRKLTAALVGLAALECALGLSVYAY
                     IPIRGFAGAWHNYGDPVHNWGDVWKLVTGARFQGLMGGSPAQLLQGSGRFLYGLATQA
                     PPPIGYALGLILFVGGIFGARALYMRDKVVGVALILALTVTLLYAISYRIADIAVYYI
                     PVYLFLGLFLTVWVTDIAGREGGRSALAAAPLCLAVLALAFNYGHADRSDYYAERQRS
                     EAIMARLPERAVLYGKLPIVPVTYLREVEGVREDVTLRWLDGGTQGRHMYSDVESGRP
                     VYVISDPRYNEIYLKSVRSYARTVDENGLIRLDPR"
     gene            complement(31396..32049)
                     /locus_tag="VFJ72_02240"
     CDS             complement(31396..32049)
                     /locus_tag="VFJ72_02240"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7478315.1"
                     /translation="MIIRHGQSTANASGVWQGQMEFPLSEEGRRQARHTGRALSGEQI
                     DAIYSSPLARAFETAEIIARETQFPGEVVSVPNLMERAGGSLEGTTVEQREAENPELM
                     ERFLSIPEEERWTLVGAETDEEVLERFERGMAEILERHGAGERVLVVSHGGSMRAFLR
                     EIFGPGALPGTQRVPNTSITRIEWSTNGLRPRLLELASTAHLPDESAARLPIGDQAE"
     gene            32168..33004
                     /locus_tag="VFJ72_02245"
     CDS             32168..33004
                     /locus_tag="VFJ72_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478316.1"
                     /translation="MFWKRNDGETREEEETPQRPSGPIQIERDAQRPATILRVAGELE
                     ERGGKILELFKEVQTSDKQTAVIPIHAARDGREFFVEVATRPWTPDSVESTLRMVSVV
                     RNSDYADTPLDILSAYPVPQDIEFFFSDSPVALFQLELMRGDQAKPEQFAEIFRQAAS
                     RHWDTELDYETSCLPLVEELLTSALDADDSGELPPILNALVEGLGCFTGEVIRRESGI
                     PAAWETAAGWGDSLILAFDDLTLDSVGKARAFLHEGKEDSISFYAEYALRELKRAPSG
                     AV"
     gene            complement(33043..33309)
                     /locus_tag="VFJ72_02250"
     CDS             complement(33043..33309)
                     /locus_tag="VFJ72_02250"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET7478317.1"
                     /translation="MERIALSVAQESSQDIKLYTGAYCPYCRRVSKELDRLGLEYESV
                     NADADGRETVIQLSQQRAIPVLTIGDEVLVDSTHIIRELRRRYA"
     gene            33618..35372
                     /locus_tag="VFJ72_02255"
     CDS             33618..35372
                     /locus_tag="VFJ72_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET7478318.1"
                     /translation="MTLKDRREKSDARENTLRNFLGGSRNSKKGGGRNFAGPIIIACA
                     VIAMIVAADYWINAGKIYRGVEVGTIALGGKTPEEAKKIITERTTGALLKEIKLSGPQ
                     DFSRSAADMGVDFNVSRTVDQAYAVGREGNLLDRLGERLKAAYGTVTIPPDVNYQPEK
                     ARVQVENLAARLDEKPKDATMDIVGPEVEIVGSKEGYKTDVAATMKSVNGAVEDMTGE
                     AKIVGEVLKPQVGTSAAKEAGEKARKAMSSQMVVTSGKQKWTVTPSAIGDALDVEKKG
                     GGIQVSLNRDRMRNNLANVYAALTVKPVEANYVVNGSKVTVQPGQTGVSVEDKKFIGA
                     IEDGIFDGKHSYTVPVTKDEPNLTTTEAEKLKPTDLLSSYQTNYLTYDDSPGRVTNLE
                     TASGAVNGTLLAPGEVFSFNALAEPLQYEKTKVIIKGKVDEAEGGGLCQVSSTLYMAA
                     NLAGLDVIERHPHYAELPYIQPGFDATVWFGALDMQFKNTSPGYVLIQEWVDDQGYVN
                     ANIYGKPSGVKVDMDSQKVSESTDSEGNPVTKWVTYQKITKNGKVVFDDVLHEDTYKF
                     LKPAEEDNATSAEVPSTQ"
     gene            complement(35442..35903)
                     /locus_tag="VFJ72_02260"
     CDS             complement(35442..35903)
                     /locus_tag="VFJ72_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7478319.1"
                     /translation="MASPSERLRELGYDLPAVPAPAGSYLPAAKSGNLVFTAGQLPFE
                     NGELRKTGKVGDVVSAEEATEAARLCALNALAAAASETGGLDSIRRVVKVTGFVASAP
                     GFNAQPQVLNGASDFIGEVFGEAGLHARSAIGVAELPLDSPVEVEIVVETG"
     gene            complement(35904..36398)
                     /locus_tag="VFJ72_02265"
     CDS             complement(35904..36398)
                     /locus_tag="VFJ72_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7478320.1"
                     /translation="MDREPQDRNSILAAMRHLTHGVYVLGTRRGRQSNAMTASWVMQV
                     SERPPSVAVAVRDDRYSRDLVLETGTFGLSVLREDQVDVATHFSETSGEYHDKLLGVP
                     YGLTPGGSPFLLDCLAYLDCRVMDTARAGDHTVIIGEVTAGDTLHESYPLIFDPTEYE
                     EALR"
     gene            36511..37488
                     /locus_tag="VFJ72_02270"
     CDS             36511..37488
                     /locus_tag="VFJ72_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET7478321.1"
                     /translation="MSGALVAIGMAQAVVYLLFIRFVDLYEREPLWYVIPVFIWGFAV
                     ATTASLFFNTIAAITISTIASTQAADTLTAIFVAPVVEESFKGLALIIVFLVALMLRR
                     RGAMEYSGVMDGIVYGSAVGFGFAIAEDLIYYAQYGAEVFVVRRLFGGFAHAAFTSLT
                     GVGIGLIPWVRSGLLKVALPLVGLVCAMLLHATFNFTATVFGSVAYLLLLCVIAAYLV
                     LIAIWLAVERRTIRDELRDEVGFGTISQEEYGILPTYFRRTAYYLGLMLRGRFGTWRT
                     ARKVHNAAVDLAFTKRLTRRSWSASGESRVDELRGRIRALRGPAAAMRT"
     gene            37500..38096
                     /locus_tag="VFJ72_02275"
     CDS             37500..38096
                     /locus_tag="VFJ72_02275"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET7478322.1"
                     /translation="MASGIILAGGASSRMGRDKLSLTVGGVPLIRRVYDVLSSHCAEL
                     LVAGAADRLPEIPVARTVEDLRPGREGPLAGIETGLTAARYSLVFVAAADLPFLPADL
                     VGYSLTLLTRERVSVVAPRHEGLHPLCAAYDRAILPQVSAALDTGERSVTRFIRGLRG
                     VRFVEGEELSRFGEPEFFLMNVNSPEDLERARACGGVE"
     gene            38141..38695
                     /locus_tag="VFJ72_02280"
     CDS             38141..38695
                     /locus_tag="VFJ72_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET7478323.1"
                     /translation="MNIYRGGEVPFRYDERAGEVRTAVILGAQVLRGGRASRTLEARV
                     RHGAELYQRGAVDLLIPTGGLGEHPPREAEIMAWLLRDAGVPESAILLEDRATNTWES
                     ALFVAEMERQESIGEIRAVTDPLHCPRTIRAFREAGLRAWAEPVYSSPMWRVPLMRRG
                     QLAREMVASVWYRVRHGIGSRSTL"
     gene            38650..39552
                     /locus_tag="VFJ72_02285"
     CDS             38650..39552
                     /locus_tag="VFJ72_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7478324.1"
                     /translation="MVQGEARDRLALHAIAVGVLAISAAAIFIRLADAPALAVAFWRS
                     ALGAVVLLPIAVARREKLPEPRVLGWLVLSGVLLGAHFGFWISSLDYTSVAASVVLVC
                     TAPVFVAILAYLTIGEKTSLVSFAGILVAVVGAVLIGGDRSVGSAALYGNVLALIGTV
                     TIAVHLLIGRRTRTGGLGVLPYSIIVYSAAALTLLPAALITGERLWGYSGETWFWLFT
                     ITLGPQIMGHTVFNWALRYVEASVISATVLAEPVAAAVLAWLILSERPGLATAIGGAI
                     VLIGLYLLIRGRRNPAAKTATKRV"
     gene            39649..40263
                     /locus_tag="VFJ72_02290"
     CDS             39649..40263
                     /locus_tag="VFJ72_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478325.1"
                     /translation="MGPLRTGFVYGLIAVLLTLVAGFRFVYLDPAGTPEWVLATTETF
                     LPLLVAAAQIFLGILAAIRTLPVRVDPDVPYRAILVRDCTLAATVVAVMVGVALILMT
                     ALHATVFADDLRAYAHDAAPRIAAYVNETGKDLREPPPPTSAGQIEKNLQPPVVEDLG
                     RSLFNLVLRALLIGAFGAGVGFIRSFSHSRRASAAAQGKPEAGK"
     gene            40274..41200
                     /gene="cbiB"
                     /locus_tag="VFJ72_02295"
     CDS             40274..41200
                     /gene="cbiB"
                     /locus_tag="VFJ72_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HET7478326.1"
                     /translation="MTDRAVQVAVALLLDAHAGEPPARAHPTVWMGRAISAFEKRALG
                     LKSPRDRLVAGAAFAFALPAVVFIATRAALSLAPRPLRITLEVVLLSTTLSMRGLADA
                     ALAVEHELERGDLESARARVGEFVGRDTATLSESEVSRAAVESVAENTSDGVVAPMFY
                     GAFFGAPGALAYKAVNTLDSMLGYRDPPYTHLGRASARLDDLANFLPARVTVLAVAAL
                     AGPEAARAAWRYGPLTSSPNAGWVEAAFAGALGVRLGGANAYGGVIHPGAILGTGRSP
                     GGRDIAAAVTLMRRVCALLACMAVLAGGIRRG"
     gene            41193..42275
                     /locus_tag="VFJ72_02300"
     CDS             41193..42275
                     /locus_tag="VFJ72_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7478327.1"
                     /translation="MVRGWAGHADGRHHGAHGAEVLRALGGTSNDFLDFSQNINPLGA
                     PARAVEAARNAGFAVYPDLGYADLRKALGEYVGVSDDYVLPTNGGAEALFLAARAAAE
                     ARSGKKAVILEPTFSEYAAAARAAGFEVERRVARRAEDAFTMDPETLGDLADVGLVFL
                     CNPNNPTGGAMTRDAMLEVSRCLAEAGVVLVVDEAFADFVPGVSVASRVGLGGFENLL
                     VARSFTKFFGIPGLRLGALISADTGLAGAFQPSWSVNAVAAAAGIAAASDQAFTKSSI
                     SEVTWLREDLYAELEALPGIRPYPGAANFLLVEGPDTLTERLARRRVLVRSCEPFLGL
                     GTGYFRVAVRTMEENRLLVEAIREEL"
     gene            42293..42787
                     /locus_tag="VFJ72_02305"
     CDS             42293..42787
                     /locus_tag="VFJ72_02305"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF014351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008820 - cobinamide phosphate
                     guanylyltransferase activity [Evidence IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HET7478328.1"
                     /translation="MGGSRSGKSAAAERMVHDLGADRVLYVATAETSGDPDLELRVRA
                     HRERRPSDWETLELGGGDLKAALDAAYRHEAVLFDSLTLWVSARMEREKETLAEFDAF
                     LEGAASMEADVVLVSDEVGLGVVPESAAGRRFRDLLGLVNQRAAASAGEVHLWVAGIG
                     VRIK"
     gene            42790..43521
                     /gene="cobS"
                     /locus_tag="VFJ72_02310"
     CDS             42790..43521
                     /gene="cobS"
                     /locus_tag="VFJ72_02310"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HET7478329.1"
                     /translation="MRALLAFFTVLPLRGATLREAAQSVHLLPLVGLVTGLPGAAALL
                     LGYALPPGVAATLGIGAVLLAAGLHHSDGVLDVGDALMVRGSRERRQEVLKDARVGIG
                     GLGALFLVYAPALTALAAIADTSPVRAALALLAAEVAVRSLMVLVLAFGEPARATSSA
                     VEFVRSLSGPRRTAGIVLALAAPLLLALPLGAAALLAALTVPLVALLALRVSGYAFGG
                     ISGDVSGATGELARAVFLVVLSATI"
     gene            complement(43574..44290)
                     /locus_tag="VFJ72_02315"
     CDS             complement(43574..44290)
                     /locus_tag="VFJ72_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET7478330.1"
                     /translation="MLGGTRINYLGHATFRFVTAGGEQIIIDPFLTDNPQTPEELKQV
                     GEADTILITHGHFDHFADAIPLAQSTGATVVANFEIFAYLQSQGVENAMPLNKGGTAQ
                     VGGVKVTAVNAFHSSSIQTEDGSSIYAGEPMGFVIEFESGFKLYHSGDTAVFGDMALI
                     GELYSPDLALLPIGNQVVMSPFEAAHAARLLGVRHVVPIHYGTFPFLPGTPEEFQKHL
                     DEIAPEVTVHVMQPGDNLAS"
     gene            44464..46260
                     /locus_tag="VFJ72_02320"
     CDS             44464..46260
                     /locus_tag="VFJ72_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7478331.1"
                     /translation="MGYLPIEDHGVIGDLHTAALVGTDGTIDWLCLPAFDSPSVFCSI
                     LDDEKGGHFTLRPTDYVRSQQLYLPDTNVLLTRFLSPQGVAEVLDFMPIHTGHGERHR
                     LVRNAHVIRGEMTFEVECRPAFDYARCPHKVSVGKTGVVFDCGNFRLGLASDTALKET
                     DGGGAGGRFTLEAGERATFVLADIAEGEGPGDILSPPEFEELLGRTLSYWRRWISRST
                     YQGQWREVVNRSALALKLMVYDPTGALVAAPTMGLPERIGGERNWDYRYTWLRDAAFT
                     LYALMAVGFEEEAGNFMGWLRDRCTQDSDGLLQPLYGIDGRLDIEESVLDHLSGYMNS
                     GPVRLGNAAYQQVQLDLYGAVLDAAYLYNKHGAPLDYDLWQNVRKILDWLSENWQRPD
                     EGMWEVRGGQRQFVSSKLMCWVALERGTRIARQRGLPGGDGHWILERDRIYEDIMQKG
                     WNPERKSFVQYYGSDALDASLLLMPLVKFVGPKDPRWLATLERIQEELTHDTLVDRYL
                     NAEAAPDGLEGDEGSFSICSFWLVECLARAGRLDEARLSLEKMFSYANHLGLYAEEVG
                     TSGEALGNFPQAFTHLSLISAAVHLDRALHHS"
     gene            46264..47166
                     /gene="ftcD"
                     /locus_tag="VFJ72_02325"
     CDS             46264..47166
                     /gene="ftcD"
                     /locus_tag="VFJ72_02325"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HET7478332.1"
                     /translation="MRAPLFEAVPNFSEGRDAARISRIVSSVRAVPGIRVLDLHTDPD
                     HNRSVLTFAGEEEPILRAAVTLAAACAAEIDLSAQAGVHPRMGSLDVLPFVPLENATM
                     DGAIRLARLAGESIGSLGYSVFLYERAATSEHRRNLAEVRRGGYEGLTERIKDPLWHP
                     DYGPNALDPRLGAVAVGARDFLIAFNAYLDTANVEIARQVARKVRERDGGLPGLKALG
                     LEVAGRAQVSMNVTDIEAAPLPVVLEAVRAAAAALGARIEWTELVGLMPLEALLQTAR
                     HHLALRDFDGRHVLEAGLWRSEDG"
     gene            47296..48123
                     /locus_tag="VFJ72_02330"
     CDS             47296..48123
                     /locus_tag="VFJ72_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7478333.1"
                     /translation="MESGRKLVAAAIQMSSTPDKDENMATAGALIRSAAAAGAELIAL
                     PELWSCHGLDKVYRENAEQIPGPTTDFLGELARELGVYVLGGSILEGASGSGRLHNTS
                     TFFGPDGELSAVYRKIHLFDVKAPDREYLESKTIVPGEEIVTAKAGTATLGLSVCYDV
                     RFPELYRLLALRGAEILAVPAAFTLQTGKDHWEVLLRARAIENQAFVIAPAQWGQKAD
                     GRWTYGRSMIVDPWGTILAQCPDRDGYALATLDLDYLDRFRTEFPALKNRRLADQGW"
     gene            complement(48142..49557)
                     /locus_tag="VFJ72_02335"
     CDS             complement(48142..49557)
                     /locus_tag="VFJ72_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HET7478334.1"
                     /translation="MIEIIGISVAVSPFVVAVVALILLRWPAMWAGVAALASAVGGAL
                     LWPSLHASGLGSSLLDGLGTSAQVLYVLFGGLLLYNLLRAGGATDEVSRFLGRLEPER
                     EALAAGVVVGVAPFFESVTGFGVAVVISAPILLAAGFPPLKAAVLASWGQCAVPWGAL
                     GVGTVIGANLADTSFQDLSDMSAYLSLPLFPVYGLAAVALAGGWAGLRRHGAEAALLG
                     LVTGVATLVTSLFLVPELSGAVGGLAATAFFLVRRRERAAGVPVRELLPYGFLLALLA
                     AANGIGTIAAALGRLGPVFTGPGLPLLMSSAFAAVTLKVGAGTAGKALCETAAQWLPT
                     AGAVLTFVLAGEVVAGSGAASLLASAAGGLGNYYLAFSPVLAGLGGALTGSNAASNAL
                     FMPLQIEAARGLGLPETLTAAVQNVAGSHASMLAPQRLVLAATATGLLGREGDLARAA
                     AAPVALALAILAWIGLVYQLH"
     gene            49578..50180
                     /locus_tag="VFJ72_02340"
     CDS             49578..50180
                     /locus_tag="VFJ72_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET7478335.1"
                     /translation="MDEGGAREVGTVVEAVSSEQLGARVRDLRRRRGLTLEALAESSG
                     VSRAMISKLERGEKNPTLVIAARLAEGLGVTLSQLAGMEERREVIVVPRDRRMVMRDP
                     ETGFERQVLSPNFAGRGVEFVRNVVPEGGTSGEFPPHKKGVEEYIVVEHGRLRAVLGA
                     EEYVLEEGDAVYFEADVAHRFDNLGEGACSYYLIISSRGV"
     gene            complement(50198..50680)
                     /locus_tag="VFJ72_02345"
     CDS             complement(50198..50680)
                     /locus_tag="VFJ72_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478336.1"
                     /translation="MADLSTTTSPKTLNCPLCGSGVLHPVGRESARCDTCSSLIGGPT
                     LKTLLDIASLPDATGNHACECGHPEMRRLPDGVYWCPACGSEVVPVSASKVSWKSPDH
                     SEAYWSGWLDGRYRSQELFTRNRSLMKWENAPERLEYYRGHRAGYEARLRKTSLLEAS
                     "
     gene            complement(50831..52000)
                     /locus_tag="VFJ72_02350"
     CDS             complement(50831..52000)
                     /locus_tag="VFJ72_02350"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET7478337.1"
                     /translation="MRGKSGGIGSLRFNTASETEIRGAEVADVYFHRTMQVLRARGLD
                     NALVHAEVAYKTSNPDEWFVVAGLDEVAHLLDGTEVEVRAVPEGTICGPNEPVLALSG
                     PYGAFAEHETAILGFLCQASGVATGAARCRIAAGDKPVVSFGARRMHPAVTPMIERAA
                     YLGGCDGVAVELAARRLGIPASGTLPHALVLLIGSTAEAARAFDETVAPDVPRTILID
                     TFDDEKFGALIAAREIPDSIYAIRMDTPGNRRGDFRDLMREVRWELDRNGFSHVKIFA
                     SGGIDVDYILHLNPVCDAYGVGGAIADAPMIDYSLDIVEVNGEDRSKRGKRGGRKQLL
                     RLDNGSHHVTPAGADIPLGASDLLQPIEDLYAAGSDVSEMRERVLAQLSTGRFTL"
     gene            complement(52189..>52588)
                     /locus_tag="VFJ72_02355"
     CDS             complement(52189..>52588)
                     /locus_tag="VFJ72_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7478338.1"
                     /translation="MKHIFLLEEQACFRQALARALEREPDLEVIGQAGSLAEARNGVP
                     GRWRAADVAIIGLLLPDGVGTDLIEDLREVNPNLPVLVLTVLDSTEFRAWAIRMGANE
                     LLGKKSASISEVIAEIRRLVGEKRLVGRTG"
BASE COUNT         8786 a        16418 c        17804 g         9580 t
ORIGIN      
        1 agctacgatt cagatctctc caacgtggag cgggtgaccg tcgaggtcgc taaccatgtt
       61 atggaggaga tagaaggcgg cgtcccagag ttcgagcctt cggtccggtt caacaacttc
      121 ggtgacttca gcattgactt cagcgtcagc atgcagacgc gggaggtcgt ggagcagtac
      181 cggatcaagc acgagttcat caagcgcctc cacgaacgct actggaaaga agggatcgag
      241 ataccgtcgc ctccggttgc cgctcttccg agcagcggca agagccaaga agcgtaacgt
      301 agacccggct caagactgct gcccgcagat acgtggccgg taccgcagtc ggtcatgcgg
      361 ctgatgccag gctgacggcc aggcttcacc ttccgcggcc gtgggttatt atctttcgca
      421 tgaagaatgt tgactctaaa ggaagcgggg cggagatagg ctcggccccg gatgaggtcg
      481 cggagcggtt gcgcgaggcc gtggcccaac cgctcgatac ctggcgcgag gtctacgaga
      541 gcttctctcc ggtggacgtg gttacgggcg agcgtatgcg gcgaatcccg ccgccatccg
      601 gcggcgcgcg ccgggccgcg gtcctcgtgc ccgtattaat agagccggat ggcgcgcaca
      661 tcgtctacac cgtgagaagg gaccacctca acgaccatgc cggacagatc tccttccccg
      721 gcggcggcat ggacgccggt gacgagtccc tcctcgacac ggcgctgcgc gaagcggaag
      781 aagagataga cctgagccgg gatctggtcg aggtggtcgg cgccctggaa gagatgtaca
      841 ttccaccgtc gaacttccac gtcagcccct atgttggtct gctccccccg gaggcggagc
      901 tggtcctcgc cccggacgag gtcgaggaga tcttcaccgt ctccctccgc tcgctgatgg
      961 ccccggagac gttccgcagg gtcctctggc ggcgcgacgg acgggaatac gaagtacccg
     1021 tcttcgcggt cgaaactccc gagccgcggc aaatatgggg cgccaccgcc ggcatgacgg
     1081 ccgctctcct cacccgcctc ggatggcgcg agtaccgtca ccagacatcg tcaatacagt
     1141 aatacagtaa gcagtaaggc cgtccccgag taccggcacg ctccgtcagg tagggcgtgc
     1201 cacagcaagc tcccaggaga agctcctagg aattacgaac agttcgagcg gcgggcttca
     1261 ggaccgcggg tatctccctg acgtccttac tttcaactcg cgagcccggt cccaggtacc
     1321 gtggcatttc cacggagacc tccggcatga acctgcgcgg catccagact ttcggttcgt
     1381 atttcacgaa aaatccactt gcgctcccct gcgccatgtg ctaacttacg ttgtaaatat
     1441 acagtgtaag tagatggtgg gacggtttgc atgtcgtttc tggggttggt ggagcgcggg
     1501 gcgcacggct cggagttcct ggttacgggg cttctgctgg cgggggtgtt gttcctggcg
     1561 ctgacgacgg ggtggttcct ggtgacgaag cggacgttgc cggtacggga cctcgaacct
     1621 gtcgaaatag tctggtattc gcgggaggcg gaagccgtgc tcctggaggt ggcgccgctg
     1681 ttcatggcgc ttgacgagcg ggagcggacg atagaggcgt acttgaggag tgtggatgcg
     1741 tcgcgggcgc gggaggtccg ggaagcggtg cggctcacgg gggtgcgcga gctttggagt
     1801 tcgttcgcgc gggcttcggc actcgtggag gacgacccct tgcggggcgt ggatgagttg
     1861 cggcggttgc gtccgatgct tcgggagtgc gtcacgtcgc tggacgggtt gatggacagg
     1921 ctgaagttgg aagtggctga agagaaaggt gagaaaaatg caaggtccta gagatgctgg
     1981 cctgatggag cggctcgcgg ccctcgaagc cgaggtcgag cgtcagggat gcgagctccg
     2041 cggcgtcatg cggcggcttg atcttcttgc cggcaagtac gctctcgcgg acggacctcc
     2101 gcatgtcccg gagcggaccg acgcgcagga gcgcggcttc tctgacgtta cgaggacgtt
     2161 gagagagaag aagttcaggt taccgcttgc tctcgaacgc ttgcggagcg gagagtggtg
     2221 gttgaacagg gtcgggatcg ggctcttact gctcggggcg gtgttcctgt tcaagttctc
     2281 ggtggaccgg gactggatca ccccgcccgt gcgcgtgggg tttgggctgg cgctcggggc
     2341 gctgctcgtg acgctcggcc tccgcgtcta tgccggacgg agatctttta gccaggtgtt
     2401 actcggcggt ggcgtcggag tcttctacat caccggctac gctgccttcg agctgtacgc
     2461 tctcgtcccg tatccagccg ccttctcgtt catggtgatg gtcaccttga tggccttcac
     2521 gctcgcattg cgtcagaacg gcgtggcgct tgcgctcatc ggtgcgctcg gcgggttcgg
     2581 gacgccgttg ctactctacg attccggcgg gagcgtgagc gggctggtgc tctacataac
     2641 cctggttctg gccggcgcgt gcgcgatcta cctgtacagg ggctggcgtt ccctgctgat
     2701 ggccgctttc gccggagtat ggacggtact cttgatcggc tcgtggagcc tgccgtatcc
     2761 gcctgatgcg gctctctctg accggctcgc ccttcaggtc ggggcagctc tcgcctggag
     2821 tatcctgtgg gtctcggcga tgggacggga ggtcttgcgg gaccgggccg cttcccgcag
     2881 aggtagtgag cactccaacg ctccggcgca cgctatctcg cacgccatct gtatcgccgc
     2941 gccgctcgcc gctctcgcct acacgcggga aatctgggac ctgcagggag acacgctcgg
     3001 ttggatctcg ctcggcgcag ccactctcta cgctcttgct tccacattta ttcttcgcat
     3061 gagcggaggt ggcttgacgt actacactca ggcggccggg gctctcgtgc tggctacgct
     3121 atcgctcgtc ctgctcctcg acggagacgc tcttctcgtc gcgttggccg ccgagggagc
     3181 ggcgctccat tacgtcgccc ggcgcctccc tgatcgtctc ctcgccgtcg gggcgaatgc
     3241 gctcctcgct atagcgctgg cgtgtatatt cacccgccct ctatgggact atccgctctc
     3301 gggccaaatc gccgttttca acacgcgcag cctgtcggac ctggccgtca tcacgctcgc
     3361 cttcgccgcc acgttctccg tgacggcgtg gcgggcggcg atcatcctcc ggctggccgc
     3421 ccacgccgcg cttctcgcgt ggttctggcg ggagctgagc gtgatccccg gcggagagcc
     3481 ctatgtcacc atagcctggg gactctacgc cgcggcgcta ctcgtcgccg gtctccgcct
     3541 cgacagcacc gcgctggctc gaacgggcat ggcgacactg ttcctcgtcg tcggcaagct
     3601 cttcctcttc gacctagccg gagtggaagc tgtctggcgc atcctgctct tcctcgggtt
     3661 cggcggcctc ttcctcggcc ttagctacta tctccactcg ctctggcgtc cgaaagcgct
     3721 ggacaccacc cagagcccgc aggttacaac tcgtaacccc ggaggccccg gagcagagat
     3781 gaagctagcg caaggtaagc gagatccaac aacatacggg gttttgaagc gcaagttata
     3841 gagttatcca caacatattg tggataagat cggaaaatgg tggataactg catcaggtgt
     3901 aacaaggctt taacggcgcc gggtcttcat ttacgcggtt ggatatggga gcgtgttgga
     3961 gtgggacggg tcgggggcgg aaggggttgc tggaagcagt ttccgcggaa cattactacg
     4021 tcttcttggc ggcgagcatc tgggaaaatc gttggagtgg cgcttccggc gttcgtaagc
     4081 gcggttacac ttgctcggct gcgtgcgggt caccggcgat tttggtctgt gaggccggta
     4141 gccagcgtat ccgtagttgg acaagagcgt tggtgctcgt acaatagatg tgcttagaga
     4201 tgactaggat ccgagaggtg gagcagtgat aagcgaggag cgcgtaaggg accagttgcg
     4261 gaacgtgata gacccggaga tcggtatgga cctggtcgag ttgggtctga tctatgacgt
     4321 gggcgtccat gatgaggggc ggcacgtgga cgttaccttc agcctgacca gcccgatgtg
     4381 cccggtcggg gacctcattc aggagcaggt cgagaccgag acactctcta tcgagggcgt
     4441 cgagaccgtc aacgcccagc tcagcttcga cccgatgtgg aacccggaga tgatgagccc
     4501 ggccgccaag ctgttcttcg gccgctagac gagcaacttt ggagcgcggc gccgggggag
     4561 atctccggcg ccctttctcc tgagcagttc gtgaacatca agggccttct cactgacctc
     4621 gacggcaccc tattcgtggg ggaggatccc gtaccggggg cgcgcgaggc cgtcgaggag
     4681 atcaagagcg cgggcatcca ccttcgttac gtcaccaaca ccacccgccg tccccgccga
     4741 gccgtggcag agcagttacg cgcgctcggc ttcgaggttg aagaccggga gatcttcact
     4801 cccgcccgag ccgctgccgg acttatcggc ggcaagagct gcctcgccct cgtggacgaa
     4861 tctttacacg aggatctcga aggtgtctcg ctgaccggag agcacccgga atacgtcctg
     4921 atcggggacc tcggcaaggg attcacttac gagcggctcg acgcggcgtt ccggcgcctg
     4981 atggacgggg cggaactggt ggcattgcaa aagaaccgct actggcagac ctccgacggg
     5041 ctctccctag acgccgggcc gttcgtcgcc gcgctggagt acgcgagcgg caagaccgca
     5101 acggttgttg gcaagcccga gcgcggcttc ttcgcgctcg cgctgcggga gatggggctg
     5161 gaagcgagtg aggcggcgat gatcggagac gacgccgagg ccgacgtggc gggagcgagg
     5221 cgtgccggat tgacgggcat ccaggtacgt accggaaaat ggcgtcctgg tactgaagag
     5281 agtggggcgg acctgaccct cgacagcttc gcggacctgc cgcgcgctct cggcctgtga
     5341 gagaatccgg gcgcgcatcc ttcaggtgtg atgcgtgtgc tccgcgaaag gttcgccgct
     5401 ctcctacgga aggatctcta tatgaaggtc acggacatac cggtccgcac ggaagaccgc
     5461 taccagctcg tctccataac cggcgaggtg cgccgggctg tggaagaggc gggcgtcgag
     5521 gaggggctgt gcgttatctc ctcgacccac accaccgctg ccataacggt caacgaggac
     5581 tacgaccgcg acgtgccgcg ggacctcgcc gctgcgtgcc gggcgtttct gaacacgttg
     5641 gacgtgaggt ttgaccacgc cgaggggaac agcgactcgc acctgttgac gagcctcttc
     5701 gggcagtcgc agaccttact ggttagggaa ggggagttgg acctcggccg ctggcagggc
     5761 gtctttctcg ccgagttcga cggcccgcgc agccgcaccg tgagagtagc cgtaaccggg
     5821 tagagttgtt acggcaccat ctgtttgcta gcttgttaca tccggtaaga tatagggaga
     5881 atcgcgtggt gattggcagg tgtccgagcg ctacaagtcc tgggaggtcg atatgaacgg
     5941 atgggtaaag agcggactcg cgctaggcgg catcgctgta ggtctcggag cgatcaggcg
     6001 gggtctgaac ccgaggcccc gctacgcgcc gtgggagaag ccgcgctacg aggatttcga
     6061 gaagaagatc ctgatcgtcg gcggaggttt cggaggttac acggcggcca ccaagatctg
     6121 ccaggccact cgcaagcggg aggacgtggg cgttcttgtt atcgcgcgcg acaacttctt
     6181 taccttctgg ccgatggtgc cgggtatcgt tagcagcgac atagacacgc gcaacgtggc
     6241 gcagccactg cggcgagccc tgatcatggc cggctccagc ttccgccgcg ctgaggtcac
     6301 gggcgtggac tacgaccgca aggtggtcat cgccaacgac gggatagagt tcccgtatga
     6361 ccagctcatt atctccgtcg gcggccagcc aaacttcttc ggaatccccg gcgtcgagga
     6421 gcacagcctc accatgaaga gcgtcgagga cgccgagcgt atccgcaacc gggtcatcga
     6481 gcgtttcgag gaagtctctc tgatccgcgg cgaggtcccg gagtccaagc tgaccttcgt
     6541 cgtgatcggc ggcggcgcga ccgggacaga ggtagcctcg gagatccaca cgctcgtcca
     6601 cgaggcgctc gcccccgatt acccgaacat agacccgagt cgcgtccgaa tattcttgct
     6661 cgaaggtctg cctcagatcc tgcccgagct ggatcccgct ctgcagcgtg cggcccgcgc
     6721 ccggctgcac tccgagcgga tagacgttag ggtcaacaca ttcgccgaag aggtcacggc
     6781 aaacagtgtc aggctcaagg gtggagaaga gatcgagagt gagaatgtta tctggactgc
     6841 gggcgcccgc ccgaacgtgg cgattcagaa cctcgatctc ccgataaacg agaggaccgg
     6901 catcaaggtg gacaagtacc tccgggtgga ggggaggccg gggatctggt ctatcggaga
     6961 ctgcgcctcc atcccggata tccgccagga gaacggcaag gtcgttccgc ctacggcgca
     7021 ggccgcgatc caggagggct acacagtggc caagaacgtg atcaaggcgc tcgacggcga
     7081 gacggacttc gaggagttcg agtaccgccc gctgggccag ctcgtcgagc ttggcagcga
     7141 cttcgccgtg aacgaggtta tgggcgtgcg cttctccggt ctggcggcgg ccctgttctg
     7201 gcggatgacg tacctggtgc ggctgaacag tccccagagc aagctccggg tcttcgcgga
     7261 ctgggtgatc gggtacatgc tgcgccccgc cgtgacccag atacggggct ccagggaaga
     7321 gacccatcgc ccttgagggg cggagaaaaa gatgctacgt tagccgcgtc cagcggcaag
     7381 caagcgcggc aagcgagcaa tgggagctac tgagcaagga tgaacctcgc caaccagttg
     7441 actctggccc gggtaatcgc ggtcgtcccg gtgatggggg cgatctactt cgcatttccg
     7501 ggacaccgct gggtggtgct cgtgctctac gtgctcgcca tcctgaccga ttacttcgat
     7561 ggcatcgccg cccggcgcag caacaaggta acaggcttcg gcaagctgat ggactccata
     7621 gcagataagg cgctcatagt ctcgctgttc ttcgcgctcg tcgggatagg gctcatgccg
     7681 tcgtggatgg cggcgatcat ggtgatccgg gagttcgccg tgacggggct gcggatggta
     7741 gctcttgagt ccggggaggt gatcgccgcg aatagctggg gcaaggcgaa gatgaactcg
     7801 cagagtgtcg ccgttttcgt gctgctgctc gggtatgccc tggagggcgg tacgggcgat
     7861 ctcttcgtcg ccgccggctg gtggctgatg ctcgtcgcgg tggtcctcac gctcatctcc
     7921 ggctggtcct acctcaagga cacgccccgg atcctctccg tgcagtccga gcgcgaccag
     7981 cgcctctaaa acctgctctg ataaacctgt aaaatcatgc ctcgatggag aacgagccag
     8041 atagacggct gagtccggcc gaggtcgaga tggtgctgcg ccgggcggcg gagttgaacg
     8101 cccgccggcg tcccaggggt tccggagaag ggacctctat ctcgccggag gtcctgatcc
     8161 aggtcgccgc cgccgccggt atcccggagg gagacgtgcg ccgcgctatg tgggacctgg
     8221 cgtccgagaa gaccgccgag ccgcatacct tcgcacgaag gttctatggt cccgcccgct
     8281 tgcgggccgt cagagaagtg gagcagcccg tcgagagtgc ccgcgagcac ctcgaatccc
     8341 tcctgcgggt ggagcagggc ctgaagatgc gtcgcaagac ggaggcgagt tcgctgtggg
     8401 acgcggggga tatgctgggc gtcgtgcggc gcgcgctgga cttctccggc catcggacgc
     8461 ttctgaaggc ccactccatc gagctgcggg tcgaagaagt cgaggaggag cgctgcggag
     8521 cgaacctcac cgccgacgtc tccaatcagc gcggcgagta catgtcgctc gggggcatac
     8581 tcggagccac gctcgcgcta ccgctcgcca tcgcggggtt ccagagcccg ctgttctttc
     8641 tcgccgtcat ccccgcgctc gtcgcgcctg gcatcggctt caagttcgcg tacctcagag
     8701 cgtgcgcgga gatccgtcgc gccctcgacg agttgctcga cgccgccgag gagggaccgc
     8761 cgcgaggtca ggagccgcgc gagcggggcg accggccgcc aggacagatc cggggcctcc
     8821 agcccatccc gcgcttcact ccacagtctt ctgacgacga gtaggcggag ctctcggctg
     8881 accgccggac gccgatggct tatatttagc ccgtgcctct agacgtactg agagagattt
     8941 tcggcttcga ctcgttccgt ccgggacagg aggaagtcat tcgggcggta ctcgacggcc
     9001 aggatacgct cgccgtgatg cccacgggcg gcggcaagag cctgtgctac caggttcccg
     9061 ccttgatgaa ggagagcctg acggtgatcg tctctccgct catctccctg atgaaggacc
     9121 agatagattc gctggtccag agctccgtta cggacgcggc caccctccac tccgggctct
     9181 cgccggagga gcgatgggag gtcgagcggc gggtgaggac cggggagatc aagatgctct
     9241 acgttgcccc ggagcggctt cgctcgctgg agttcgtgct ctcgctccgg cgctccggcg
     9301 tcgggctctt cgtggtggat gaggcgcact gtatctccga gtggggacat aacttccgcc
     9361 cggactacct gttcctgccg cgcgccgtcc gcgatctcgg ctctccgccg gtcctcgcgc
     9421 tcaccgcgac cgccacgccc cgcgtccggg aggatatcgt ctcctccatg cggctccggg
     9481 aaccgaaggt cgtagtaaca ggattcaacc gtccgaacct gacctaccgg gtccgcgagg
     9541 tgcgccagaa gaagcataag ctggaccata tcctggacat catccgcacg tctccgccgc
     9601 cggggatcgt ctacgccacc acccgcaagg agtgcgagga attgacggcc ggcctgcgac
     9661 gcgctggcgt cgaggccgcc acctaccacg ccggtctggg ctccgccgaa cgctcttcgg
     9721 tgcaggaacg tttcatgacg gacgaggttc ccgtcgtggt cgccaccgtt gctttcggga
     9781 tgggcgtaga caagcccaac gtgcgcttcg tgatccacgc cagcgtgccg gggagtcttc
     9841 cagcgtacat ccaggaggca ggcagagccg ggcgggatgg cgagccgagc gagtgcgtgg
     9901 ttctgtaccg gaacgaggac atcggccgca ggaagcgcct tattagtctc aacgccgccg
     9961 gaggcgcgga ggtcgagtct ttcttccggg cgctggtggg agtcgagcgg gaagggaggg
    10021 taagcgtccc gccgggttcg ctggcggcgc tcggcggggt cgagcccgac gcggccggca
    10081 tcatgctgcg gagccttgag gagtctggtc tgatctcccg cggctacgac ctgtgggccg
    10141 aggtggaggt gcggcgcgta gaggaggagc cggatggtct gcgcgacgag ctggccgccg
    10201 tccacgccgc gcttcccggc tccggcctga tcgggctccc ggacctcgcc agccgagcgg
    10261 gtcttcgtcc cgccgtcgcg cagggcgctc tctaccggat gatgctcgac ggtatcctcg
    10321 aagccgtgcc gcgaggctcg ctggtggacg tgcggctgaa gaccgccgcg ctggacgagc
    10381 gtatccgccg cacaatcgcc tcccggctaa agagccacga ttccgcggct tactcgcagg
    10441 tccgggacgt ccaggactac gcgaacctct cgaagtgccg ccgcgaatat ctgctgcggc
    10501 acttcggcga caccgaggag gtcgctccgt gcgccggatg cgacgtgtgc ctgggcgaga
    10561 ccagccgtca ggtcacggtg tccgggacgt ccgggacttt ggacctccac gcaagttccg
    10621 gccaggtgga gagcgccgct ggtccggtag acagcgagct tctggagcgg ctgaaaagct
    10681 ggaggagtga gcaggccagg gagcagaaag tccccgccta cgtggtgctg cacaactcgc
    10741 acctggagga gatctccatc cgcaagcccc gctcggtcca cgagcttggc tcgataaagg
    10801 ggataggact ccgccgcgcc gcccggtacg gcgaagagct ggtcgggctg gtacacggga
    10861 acgctgagac cgcctcccgg ccgtcaggta attcgtaccg ggagcacctg gaagccgcag
    10921 agcgcctcct gcggtccggg cgcggcgccg acgccgtccc ggaactggtc cgcgcattgg
    10981 agagcggcgc ggaagaggcc cggatggccg tggacgacat gctgaaacac actacggaga
    11041 aacgggatac cgcatgagag aagggagcaa gtcttgaagc tgcgtggcaa gaagatagcc
    11101 gtattgatcg cggagggttt cgaggacctg gaatactggg ttacggtgat gcgtctgcga
    11161 gaggaggggg ctgaggtcgt ctctgtcggc ccgaacctcg atactgtctc cggcaagaac
    11221 gcgctggagg cgagcgcgga cgttacggcc gacggcgtgg acgcggcgga gctcgatggc
    11281 gtcgtgatcc ccggcggctg ggcgccggat aagctccgcc ggtatccgga gatcacgaac
    11341 atcgtgacga cggtccacga acggggcaaa acggtcggca ttatctgtca tggggggctg
    11401 gtggcgatca cggccggcat cgtgaacggg gtgagggcga cgggatcgct cggcataaag
    11461 gacgacctgg agaacgcggg cgcgacctgg gtggacgagg cggctttccg cgatgggaac
    11521 ctggtctggg gccgcgtcgt ccccgacatc ccggacttct gccgcgagct ggtcgccgcg
    11581 ctctcagagc agagctaggc ggccggccgg gtgtccgctc gtgaagccgc ccactgggac
    11641 ggccgggacg cttggtagaa tgatcccatg agaagcgtgc tttcggtcgg tttcggggtg
    11701 gttctggctc tgctgctggg ggcggccctg gtcttcggga tcttcgcgcc cgtgctgtcg
    11761 gtgatcttcg gactggagac ttcgcggtcg agcgcgatgc cggccatcct gcccctgata
    11821 gtcttcgccg ccggattcgg tttctacttc ggcggcatgc tcgccgcgta ttacgcccga
    11881 gagcggcgcg ccctgcacgg cgtcatggtg gcggtcaccg cgttcggcat ctcgccggtc
    11941 atcaacctgg cgagcggcaa gggccctttc ccagaggtcc gcacggcccc gacggtgacg
    12001 ttgctgctgg cactgctcgc cgtctcggtc ggagccgctt tcgtcggcgc tcgccggggg
    12061 gcggcgctct acgcttacaa ccttagctac ttgcgcaaac gcaaggccgc cgaacgccgg
    12121 tcggaggagc gagaggagcg gaagccgtaa gagaggtcta ccgggcgcgg atgcccagcg
    12181 tgaggcgtcc ttcggcgtcc atgcgagcgt tcggctcgcg gccgggatgc tgcacctccg
    12241 agggttcgtc gcccgccccg atcaggtaaa cgcgcggttc atccacgccc tccgtcgggt
    12301 cagaaacgta gcgtcgccac gccatgtcgc cccagacgaa ggtcaggacg ggcttaccgg
    12361 ctggttcgtc ctcgatcccg gcgtggaccc gcggcagtcc gagcgcgcgg gagatggacg
    12421 ctacggtgcg agtgtgctcg ctcgcgttga ataacgcgag gccggatctc agccggtgcg
    12481 agggatcgga gaggcgttcg aggctctgac gggtgcgctc cagttcctcg gcgttcagcc
    12541 ggctctcttc gcgggcctcg cgaagttgct cctctagcgc ctgcgcgcgc tcctcggcga
    12601 gcacccgctg cgagtctagc tccgccagcc ggccgtcgcc ggaagattcc gcaagctctc
    12661 cacccggtct ctcgtccagg cgcggtctct ctccgttctc cgaacgggcg cggtactcgt
    12721 caagatcctc ttccaggccc gcgcgcgtcg cctccgattc cgccagggcg tcttccaact
    12781 cggtgatgcg gttttcgagg tccgagatct cctctccaaa acgctcgact atgcgttcca
    12841 ggtcggcttc ggactcggcc ttctgggcga gccttcgctc ctccagttcc ttgagccgcg
    12901 ccgcgtatgt ccggcgctcc gaggcccgct gcactttctc gccccccagc ctctcttcca
    12961 gcgcccaggt ctcggatttc ctccgctcgt cttcggctgc caggcgctcc tcgtactcgc
    13021 gccgcacgcg ggcgagatcc tcctcgtgcc gggcccgcag cgcccggttg tccgcctccc
    13081 gttgagcttg cagggcgctc aggcggcgct cgccggcctc ctttacctcc gcgagcttcg
    13141 ctccgtgcgc gcggcgcagg tcctgctcgc gggcctcggc gaggcgcttg atatcgcgca
    13201 actcctcctt gcggcgatcc tcggcctcgc tggtgcgggt ggcgatctcc tcccgcagag
    13261 cctctatctc ggcctgctgg gtctccacca cggcctgata gtctttctgt aactccagtt
    13321 cgcggcccgc cgccgcgttt cggatggcct gcagcgtctg ctcccggctc tcgcttgccg
    13381 cctgacgctc ttcgtccagc ctctcctcga acgagtccgt gagccgctgt tcgagctccc
    13441 ggcgccgctc ggcggcctgc tctctcaact cgtccagctc gcgctggtgg cgctcctcct
    13501 ggtcccggta ctcctcgcgg agcttctcct cccactcgcg agccgaggtc tttagagctt
    13561 ccagctccgc cttgtgccgc tcctcggagg cctcgcgctc gcgccggatc tcctctttca
    13621 gggtgatgaa gcgttcctcc gaggttgccg cttccacctc gacccggcgc tgctcatcca
    13681 gttcctggcg ctgagcagcc atcgcgttta tgtggtcgtt tcggagcctc agttgctcgt
    13741 cggagttcgc gatgcggagg tcgtttagct ctctgcggag gtcggagatc tcatcgtcca
    13801 tcctctcgcg tacccggtcg gcctcctcca gggcgccccc gcgggccgac acctcttcct
    13861 gggccgcgcg cagggcgatg ctcgcctctt cgaggtcccg gcggaggttc accacctcct
    13921 cttccaggct gtcgatcagg gagcccgccg cagccatctg gctggcgatc tcgcctacgg
    13981 ggtcccggat atcctcgcgg acctcctcgt tgctctcctc gcggggctct tcgctcattg
    14041 ccgcgtctct cttgtgtctt ccggcgtcac cgaacccccg cccctagtta ccacgaagca
    14101 catctggcct agagtaaacc gtctaaagct tgaggtaaag tcttggcgcc attctccgaa
    14161 cggcggagcc gggatccgtg cttcgcgcgc cttaccgctc tctccggcat ctcggacaca
    14221 cgccctccac gacgagcgtc gcgctcgtga cttcgtagga ggtattctcc tttatctgat
    14281 tctccaggcc gtcgagggag acgccgggga tctcggcgac ctctccgcag tgtccgcagc
    14341 ggatatccag gtgcggctcc acgttcaggt catagcgggt cggagactcg ttccagtgct
    14401 tggagccgat caccccgatc tcctccagca ccgatagagc ctggtatacc gtgcccagcg
    14461 ccacgcgagg gttcttgcgc ttgacgatca ggtacacgtc ctccgccgat ggatgcccgg
    14521 acgacgcctc cagcgcctca agcaccgccc ggcgctgcga ggtcaacgta taccccgact
    14581 ttcggaactt gtcccggatc tcgccgtcca tcatgcccgc gggctacctt ctgagtaatc
    14641 aataaccatt cctaattccg agtttatcac agagggtttc ccgcgcgccg gggtgtaaca
    14701 ttagggtatg ctctcttata tcctcgatgt actggtcaac tcctggcgct tcgtgcgcaa
    14761 cgtcctggcg ggtctgaggc gtccgccgga gtacatcgcg atggaagtcg gcggctcgct
    14821 cccggagttc gagcagcccg tgagcttcct gcgccgcagg ttctttccag tcccaacgcc
    14881 aatcagcatc gagcggctgc gaggccggct ggacacgata tcggaagacg gccgtacaac
    14941 ggggatactg ctgcgcgtcc gcaacctgga cgcgagttgg gccgccctcg aagaagcgcg
    15001 atccgagttg gctcgcttcc gggagcgcgg cgggcgcgtc gtcgtctacc tcaccgaccc
    15061 ggacacccgc gcctactacc tggcgtgcgc cgcagatgag atctacgcta cccccatctc
    15121 gacggtcaat atcgtcggcc tgcgtaccag ggtgaacttc ctgcgggacg ccctgaaaca
    15181 ggtcggcgtc gaggcagagg tggtagccgt gtctccctac aagtccgccg gggatacctt
    15241 cgtccgaaac gacttctccc gcgagtcgcg cgagcagacc gagcggctcc tcgaccgccg
    15301 gttcgctgaa ctggtgagcg ccgtggccgg gggccggaag atgaccccgg acgaggcgcg
    15361 cgagcgcata gactccgctc cccacacggc gcccgaggcg gtttctcaga agctgctaga
    15421 tgcagcctgc tacgaggacg agctatacga caggctcggc gccggagacg agcgggcgaa
    15481 catcgaggag tggggggctg cgcggaaggt cctgcgcgtg ccatatcgcg agcgcgcccg
    15541 aaaggtggtg gggctcgtcc gtctatccgg ggcgatagtg cggggcagga gccggcggct
    15601 gccggtgccc gtaccgttcc tcggaggaga gcaggctggg gatgagtcgg tggtcgcggc
    15661 gctgcgggcc gcggagaaga gccgtagggt ggcggccata atatttcacg tggactcgcg
    15721 cggcggcgat gcgctggctt cggacctgat ctggcgcgaa atcgaacgca tccgggccaa
    15781 aaagcccgtc gtggtgctga tgggtgaggc ggcggcgtcc ggcggctact acgtctccgc
    15841 tgcggcgagc cacatcgtcg cccgctccaa caccatcacg ggctctatcg gcgtgatcat
    15901 tatccgtccg accaccgcgg acctctatgg caagctcggc gtcaaccccg ttgccgtcga
    15961 gcgcggagag cacgcggggt tcctcgatcc ctcgtaccgg cccacgcccg aggagatctc
    16021 ttcgctcacg ggacagctcc gctttatcta tggcgagttc aaggaccgtg tggggaaggg
    16081 ccgggagatg agcgtcgaag atgtcgaaga catcgccggg ggccgggtgt ggaccggcgg
    16141 agaggcgctg gagcgcgggc tggtggatga gatcgggggc taccggagcg ccctgcgccg
    16201 cgcgcgggag ctggcggaga tcggcgacga tgtgccggag agtgtcgtga ggatctcacc
    16261 gccgcgtaac gtccggccgg cgcccggaga accggctccg gcggctatcg aagccctcaa
    16321 gcagaccttc gacctgaccc ggacgaccag gatctgggcg atctcgcctt acgagaccaa
    16381 cggagactga taacatcttt ctcgccgccc gcgagtagat gaaggagcat ccctgagcgc
    16441 gactatgcaa aagggcgtgg accggagagc gatggccacg ctctcggccg ggcacttgtt
    16501 tacggacctc aaccagggcg cggtcgcggc gctcataccg ttcctggtag cggagagggg
    16561 gctctcgctg gcggcggcgg gctcgctggt tctggccgcg acggtcagct cttccgtggt
    16621 gcagccgctg ttcgggatct tctcggataa ccgtccgatc cccgcgctga tgccgctcgg
    16681 ggtgctgctc ggcgggacgg gaatggctct cgccggcgtc gcgccgacgt tcttgctcat
    16741 cctcctgtgc gtaacgctga gcgggctggg tgtcgcggcg ttccacccgg aggcggcccg
    16801 ctttgcgaac tacgtctccg ggcctcgccg ggcgcaggga atgagcttct tctcggtcgg
    16861 cggcaatgcc ggtttcgcgc tcgggccgct cgtcacgacg ccgctggtcc tcttgttcgg
    16921 cctgacgggc acgctctttc tggtcattcc gggcctactg atggcggtgg tcctactggt
    16981 cgagataccg cggatgcgcc gcttccaacc cgcggtggcc gaaagcagga gcgacgaacg
    17041 cgccgcaaag aagcttccag agcactgggg gccgttcgtc cgcatgatcg gggtcgtgac
    17101 cgcccggtcg ttcgcttact tcgggctggt cgcctatgtc gcctcatatt acgagaaggt
    17161 cctcggcgtg agcgcggcgc tggggaacgc ggcgcttacg gtcatgctgg cgagtggcgc
    17221 ggtcggtacg ctgttgatgg gtccgctcgc cgacaggtac gggcggcgag ccgtgctcgc
    17281 cgtctcgatg gcggtgctgc cgccccttat cttcgtgttc actctcctcg gaccactggc
    17341 cggaatggtg gatctcgtcc tcgtcggagc ggcgacggtc ggtacgttcg gcgtgacggt
    17401 ggtaatggga caggagtacc tgcccggcag gatcgggctc gccgccggca tcacgatggg
    17461 gctctccatc ggtctcggcg gtctcggagc gcccttgctc ggcgccattg cggacgctcg
    17521 cggcctccac ttcaccgtgc tggtgatcgc cgcgctgccg ctcctgggct tgctgttatc
    17581 gcttactctc ccgcgccaac acccttcggt gtaaccattc gtgtaataga gatactagtc
    17641 cggccgtgaa actcggctac catatggttc gatgaagcaa ccagttagta gaggcatgaa
    17701 ccggcgttga gggcgttcga gcgcctcggc gtcttcgtat accgctaccg atggccggtg
    17761 ctcgtgttct gggccgcgct gctcctggcg agcgcgttct tcgcaccgag gctgggagga
    17821 gagctaaagg gtggcggctt cgacggcgcg aactcggagg ccgagcgggt acaggacctg
    17881 atggtggacg agttcggcgt ctcgcccgcc actctgacgg ttgtcttcga cggccggggg
    17941 cttcccgcgc gcagccggga gttccgggag aaggagcgcc gggcgctgaa gaacctccgc
    18001 tcgctggacg atgtccgcta cgtcaccacc tatgaggata cgaaggacga gcgctttatc
    18061 tcgaaggacg gcaagaagtc ctacgccctc gtcggcttcg atatctccat agatcagacg
    18121 caggacatcg tggacgaggt gcgcaacaag gtgcgccacg atgagttgaa gacctacgtc
    18181 accggcgccc cggccgtcta cctcgacatc cagacggcga gcaacgagga catccgccgc
    18241 gccgagaagt acgccttccc gctggcgctg gtgatcctgg tagtcgcctt cgggacgctg
    18301 gtggcggcta gcatcccggt cgtcatcggc ggtgtgagcg tcgcggcttc gctggcggcg
    18361 ctctacttcg tagccggctc ttacgacatg tcggtcttcg tgttgagcat cgccaccatg
    18421 ctcgggcttg ggctcgggat cgactatgcg ctcttcgcgg tgagccgttt ccgggaggag
    18481 ttggaggatt ttccggtccc ggaggcggtg ccgcggatgg tcgcgacggc tggacgttcc
    18541 atcttcttct cgggtacggc tgtccttatt gggctctcgg ggttgctctt tttcccgtat
    18601 atgttcatgc gctcgatagg cgttgctggc gtgatcgtgg tcttcgtctc ggtctctgcg
    18661 gctctgacgc tcctgccggc gctgctcggg gtgatcgggg ccaggatcaa cgcgctcgcc
    18721 atccgccgcg gcagcacggc gccgggtgag agtttctggg gacgaagcgc ggaggtcgtg
    18781 atgcgccatc cgttcgccgt gatcctgctg gtcggggcta tcctcgccgc gctgctctac
    18841 ccggtcggac acatgaagat cggcataccg gaggcgagcg tgctgccgat gaagtacgag
    18901 tcccgcgccg gcgacgacgt cctgaagaag gacttcgact acgccagcct cacgcctatg
    18961 gagatcgtag ccacgacccc cggagatccc ctgggcgcga agagcctggc agacgcgaag
    19021 gaactaggga agcgggtaga ggcgacgggc ggcgtctcta gcgttgagag cgtctatacc
    19081 gttggcgagc agggcgcgaa ggactacgcg aagcgcatca cggaggcgag agagaagggc
    19141 cgggcggagg ccgcccgcca ggtggacgag ggcgtgaaga aggagctgga caagctgcgg
    19201 gaccagttcg gggtggtacc gcccggcgcc gaggagcggg tccgggcgga ggcggagcga
    19261 cgcgcagacg agaaactgcg gcgcgagctg ccgaaactgc ccgaaggcat ctcggccagc
    19321 ggcaaggtca cgcccgaggg ggttgcgaac ttcctggctc tgccggacgt gcgggaccgc
    19381 aaggagatcc aggacgctcg cgatgcctac gtggcgaagg accggactct tctccgggcc
    19441 gttacaaaag agaaccctta tacggaggaa gcccgcgccg ccatcgagga ggtccgttcg
    19501 gtcgatccgc cgccggggat gagctttctg gtcggcgggc tctccgcggg acagcgagac
    19561 ttcatctcca gcgtctacgg gaaggcgccg ttcgcggcgg cgttcgtcct gggcgtgacg
    19621 tacctgatcc tgttccttac cttccgatcc gtgttcatcc cgctcaaggc ggtcgtcgtg
    19681 aacatcctca gcctgacggc gagcttcggg gcgatggtct tcgtcttcca ggacggcaac
    19741 ctctcgggtc tgctcgactt cacgccgctc gggttcgtgg acgcgacctt gccaatcctg
    19801 atgttctgca ccatcttcgg cgtctcgatg gactacgagg tgttcctcct ctcccgaatc
    19861 cgggaagcct acgagaacgg cgactccaac accgcgagcg tcgcgaaggg gctcgtggcg
    19921 accgccggga tcataacctc ggcggcggcg atcatcatag tggtaacggg ggctttctcc
    19981 ttcaccggta tcatcatcac gaaggcggtg ggcctagggc tcgctgtggc cgtgttcgtg
    20041 gacgccacga tcatccgcgt cctgctcgtt cccgcaacca tgcgcatcct gggcgactgg
    20101 aactggtggc ccggcggcag gaaacgcacc ttccgggccg gtccgaaaga ctcgtcctag
    20161 agcctggagt gtccggcgtt cgaggtcccg gtctcttccg aacaggatta attcttgatc
    20221 ctggtgaagt agagggatag actagggctg tgatggagtt cgagcaggtt ctcaggagcc
    20281 gtggataccg gctgacgaat cagcggcggg tgatcgtgcg cgagctggag ggtgagaggc
    20341 atctgtcggc tgaggagatc tacgaccggg tcaaggggga gcatccggag ctgggcctct
    20401 cgacggtgta ccggacgctg gacctgttgc acgagctcgg tatcgttcgc aaggaggact
    20461 ttggcgaggg ctactcgcgc tacgagctat cgaccgagcg gatgcaccac cacgcccgct
    20521 gtcgggagtg cggggtggtc atggagttca acgaggagct aatggagtat ctcaggctcc
    20581 aggtcgagcg cgagaccggc ttcgtgactg agtggcacga gatcacgctc cacggccgct
    20641 gcgccgagtg cgcccgtgcc aacccttcct agacgttaaa ccggaagccg tcctcggcga
    20701 gtattacctc tccgccgtac ccgtggctga cctcggccgc caggtccaac tcgtccgcag
    20761 ccggatagat gtgcgtgaga acgacccgct gcacgcccgc ctccgaggcg atccgggcga
    20821 cggcggaggg ggtgagatgt ccggggaccg ggctctcatc ggggaaggaa cactcgatca
    20881 ggagcgtgtc ggcgccgcgc gccagctcca cgacggactc cgcgtactcc gtgtctcccg
    20941 tgtagacgaa ggttccatcg tctccgtcga gcctgtatga gatgctctcc gggcggtgcc
    21001 tggcgggcgc ccacgagaga tggcagccgg gcaggtcgag gggagaatca caggcgtgtg
    21061 gcagctctga gacctcggtg gggtagtcgc cgatcatcca ctccccccac acgcgcgtga
    21121 tggagcgcca gaagtctaca aagggctgcg ggccggcaat gcggagggtg cgggttcgag
    21181 gttcgtccga tccgtatttc atggcgaaga ggagcggaac cacatccacc gtgtggtccg
    21241 ggtggaagtg ggtgaagaat acgcggtcgg tagcgagcgg gtccagaccg gcccggacga
    21301 taccgcgcac cgcgccggaa ccgaggtcga agaccagcag ctcgccgccg gtctcgacca
    21361 ctacgcagct ctggcgccgt tccagccggg gagcggccgt acccgttccg actacggtta
    21421 cgcgcatctc aggcggctcc tttcttgctc cggaacgtcc aggcgaggcc cacgatgccg
    21481 agcagcacgc cgccgattac gtccgagggc cagtgaactc cgagatagaa cctgctgtag
    21541 ccgatgcacg ccaggtagat tacgacaagt gaggttacgg cgcggctgcc gctccacagg
    21601 cacagcgacc cgaggagaat ggtggagcgc agcaggtgtc cgctcggata cgggtacgga
    21661 tacgagatcg cgacgagcgg cgcgaagtcc tcgctccttc ccaggctctc cgggatgggg
    21721 tggaccggca ggagcatctt gagcaggtac tcgatcagag ctgttgccag gaacgcggcg
    21781 atcaagcgcg tccccagccg ccgatggccg cgcaggatca tcgcggccgc gacgcagatt
    21841 agagccagac tcgtcagctc ccaccctccc gcaaccgaga ggtatcccca caggcggtcg
    21901 agccacactg ttggatggcg ctgcgcggca tggacgagcg ccaggtccgc gctatacgtc
    21961 cacccggctc cggctaccgc cgagaacagg gcgaagacgg tgatggaggt ccagagtccg
    22021 gtgctcgtag tggtcttcaa agcaggtaga gactagcaga tcgggcaagg caaagggacc
    22081 ggaccatccg gcccggtccc cgattggaag aggtctggcg cgctgtcagg cgacggcgtc
    22141 gcgcaggacc cggccgtact cgccgctctt caggatgcgc tcggcttcac gctgaagctg
    22201 gcgcacgcgc tcgcgcgaga tctccagttc gtccccgagt tccgctagcg tggccggctc
    22261 ccgatcatcg agcccgtagc gccgcaccag cacgtagcgc gccctgtcgg ggaggcgctc
    22321 gatcgcctct tttagctgta ccgtctccat ctccttgagg accgtgtccg gggtatcgga
    22381 gacccgctcg tcctcgataa agtctccaag ctggctcgcg gtatcctcgg tggatatcgg
    22441 ctgatccagg ctcgtggcgt cgggcatcgc gctcatggtg agccgcacct cgtccacctc
    22501 ccactccagg cgccttgcga cctcttcctc ggagggctcg cgctcgaact cgacggagag
    22561 ctcgttgtag gcgcgggaga ccttccggat cttctccgtc atatgcaccg gtacgcggat
    22621 cgtgcggccc ttgtccgcga ccgcccgctg cacggcctga cggatccacc acgtcgcgta
    22681 ggtcgagaac ctgaacccac ggtctgggtc gaacttctcc acagccttca tcaggccgat
    22741 gttgccctcc tggatcaagt cctcgaacgg cagcccgtaa ccccggtatt tcttcgccac
    22801 gctcaccacg agccggagat tcttctctat caaacgctgc cgggcacgct tgtcgccggc
    22861 ctttgcccgc tgggagaggt cgatctcctc gccgtgcgtc agcagatttc cctgaccaat
    22921 gtgtgagagg tacttggcaa gcaactcggg cgtctcgttc tcccgatcgg ctctggcgcc
    22981 actcagcttc ttctccgcac tcaccgccaa ctcgtcctcc ttgaaaccgc gcttctcatc
    23041 taaaatcagt tttagaaact aatcttgatt ttacctttgt atcgaattct ctaaacagta
    23101 ttttcgggtc atttatcgtc tctcctcccg tcataaaagc ctgtgtcgag ttatacgcag
    23161 atagctacta gaaagtttca agccccaggt tcgtgagcga gggtatactg cccatatgga
    23221 gaaggaacat cagataccgg cgttgatccg ggaccgtcgc gatgagatcg tcgcggaatg
    23281 gcgtcgtagg cgtgatggcg tgatggacga tgaggcgttc gcgcgcctgg tggctggcat
    23341 gggccgtctg gttgatatat tcgccgactt cctcaccagc cccgactccg tggagacgtt
    23401 cagcaaggga ggagcaattc acgcgatggt gggtgacatc gccagcgacc agcacctcct
    23461 ggggcgggac gcggtgggcg tcatcgaaga tttctcggct ctgcggcgga cgctatggct
    23521 ggttgtcgag gagagtgtgg acctatccgc gcgtagcggc ggcgaggtcg cggacttctt
    23581 cgtcaagctg atgcaggcgt ccgactgggt aaccgggtcg gctctcgaag cctatgacga
    23641 catcgtgcgc agggagatgg aagaggctct gggagaggcg gcggggaccg acctcgtgac
    23701 cgggctgccg gagcaggatc tctttacccg gcttttgctg ccggacgcta tcgccagcca
    23761 cgaacggttc tcgctcgccg tcttcgacgt cgctggtttc tcggagaccg tggccgccgg
    23821 cgaggtcaag cgggcccgga aggtgttgcg ccggctggcg gacgccgtgc gtgaagcggc
    23881 cggggagcac gccacattcg cccgtttcgg agacgatgag gtgtgcgtat tgctgcccgg
    23941 tcaggggagt gagcgcgctt acgacgtggc ggagcgcgtg ttggaccggc tggcgggcag
    24001 aaaagactcc ttccaggtag acgtgggcat cgccgagtat ccggctcatg gcgaggaagg
    24061 ggaagccctg atcgtccaga tgctccgggc gctcaagatg gcgaagcgcg tgggtggcag
    24121 cgggatagtc gtggcgcggt gagtgaggag agcgtgtgat cgcgataatg aacagcctgc
    24181 ccgtcaagga aggcgcggcg gatgagatcg tcgagcggtt ctccgggagc gggggacacg
    24241 tccaggactt tccaggcttc gtctcaatgg aagtgatgaa gtccgaagag gaagacgaag
    24301 tgctcgtgat cacgcgctgg cgcgaccggg aagcgttcga ttcctgggtg cgtagtgaag
    24361 agttcaggaa ggctcatggg cgcggcggcg cgggtgagtt actgcgggga catccgaaga
    24421 tgacctccta tgaggtagcc gtcgaacgcg ctccgggagc tccgtagaga aagttctggg
    24481 aggtaattca gcccagcacg taggcgacca ccggctcggc gctcgcgcct ggttcgtact
    24541 ccccgcggcc gaggacgaag aaggtgatcg cgaacagcgt gataagcacg aagatcagca
    24601 gaccaaacgc gaatctttcc cggctcaccg tctctcctct ctaagcaaca ctgtcgacag
    24661 caattctacc tcaatccgcg ctcgacgtta ttccgcggag ggtactcgaa gcgcggtgat
    24721 gtacaatgtt gcaacctgta gcagcattct acgatgaggg gttcaaaatc agcggattgt
    24781 tacatgtcgg cgataccggg gcggtttgag gtggacatag atcccgcctc tcccgagtac
    24841 acgatgctcc aggagcagga gtgccgggac ctgctagaca tcctcagcga actcggcata
    24901 gctactgcgg accggatcta cgagccgggc gacgccgtgt accgggaggg agagtacggt
    24961 gacgctctct atgtcttgat atcgggcgta ttgaagctgt tccggccata ctccgggagc
    25021 aaggaggcca cgctgcggct gctcaagtcg tgggacatct tcgggcacct cgctttcgcg
    25081 ggcgagacca agcagcgcgc ctatgccgag gccgttacgc gatgcaaggt cacgaaagtc
    25141 ccgaaggttt tcgtggagcg cgctgtacgt caggagccgc gcgtggcttt caaacttatg
    25201 acgctgctgg agctgcggct ggtgcagtac gaggagctcg tgaagtgcct ccttccgcgc
    25261 gagacggagg tccggctggc gaacctgctg ccgatactcg cgcagaagtt cggcgagcaa
    25321 aacgggagcg gggagatcac gataaacctc cgcctgaccc atcaggacct cgccgcgatg
    25381 gtcgcctcga cgcgggagtc cgtcacgaaa gtcctcaacg agttgcgtaa ccgcgacgtg
    25441 atcgaggtcg aggccgggcg cataacgcta aaggactccg aggtcttgtc ccgtctcgga
    25501 cgcggctagc cggcaggtga accctgggct ctgatctgtc atagtacgcg ccggatcaga
    25561 gcaatggagc ggattcggat aaagggtggc tgccgtgctg gagttgtttg aggcgatatt
    25621 cctgggtgtg gtgcagggac tgaccgagtt cctgccggtc tccagttccg ggcatctgtt
    25681 gctcgctcag tacttcctgg gggtggacca gagccgtttc gggttatctt tcgacgttgc
    25741 gctgcatctc gggacgctgg tcgcggtggt ctcgttcttc tggagagacc tggtcaggat
    25801 ggccctggct ttcctgcgct cgttgcggca cagggacatg acggatcagg accagcggat
    25861 ggcctacctg gtgatcgcct cgactgtccc ggccgccctg atcgggttcc tgctcgaaga
    25921 cttttttgcc agtgcggtcc ggtcgccgtg ggtggtcgcg tttaacctgg taatagtggg
    25981 cgtgctgttc atagtcgccg agaccgtggg aagtcagagg cggccggcct acaagctgac
    26041 gttccgggag gcgctcggga ttggtttcgc gcaggccgct gcgctcgtgc cgggcgtctc
    26101 gcgctccggg gcgacgatga cgctcgggct cttcctgggg ttgcgccgcg aggaggcggc
    26161 ccggttctcg ttcctaatga gcgtccccat catcgcggga gccggagggc tgaagctggg
    26221 agaggtcatc gccggaggga tggacgtctt cgagactttg ctgttcggtg tagggttcgt
    26281 tacctctgca atcgtcggat acctggcgat ccggttcttc atccgctaca tcgccggcca
    26341 tagcctccgc gccttcgcgt actatcgctt cgcgctcgcg gcggtggtcg tccttctgct
    26401 gctcttgttc tgatcccgcg tagtttttga cacgaacgtc ccatctctgg taatttgggc
    26461 tcgatatgcg gtttcctggc atgacggtca ttttttgcgg cccggcgagc ttccggcgat
    26521 ttatccggga ggggcgcggc catgccggcg gcgtgtccgc gtctctcctt agctgatcta
    26581 ctacaattcc accgacacag gaggccgcgc cccagatgtt ccgccaaacg aaccccgatc
    26641 atggaaagcc gataaagtga gtacgcatgt ttgctgagaa agcctcgggc aacccggaag
    26701 agatgaacga gatacgggct ggaatagacg aggtagacgc ggagatcgtg cgcctgctcg
    26761 accgccgggc gcggctggcg cgccggatcg gggagataaa gcaggcgcgg ggtctggaca
    26821 cctacgctcc ggcccgcgag cggatggtcc tcgaccgggt acagtcccta agcgaggggg
    26881 atttcccgcg cggcggcctg gaggccgtgt tccgggagat catctcctcc tctatctcgc
    26941 tcgaagcccg cctgaaggtg gcgtatctgg gcccggaggc cacgtttacc cacgaggcgg
    27001 cccgccggtc ctttggttcg agcgtcgaac tggagccgca gacgacggtc tccgaggtct
    27061 tcgccagggt agagcgcggg gaggccgagc acggcgtcgt gccggtggag aactcgatgg
    27121 agggggcggt gacgcacacc ctcgacgagc tgatgaacag cccgctcaag atctgcggcg
    27181 aggtctacct cccgatctcg cagaacctgc tctccggcgc cgcgtcgctc gatgaggtcg
    27241 ccgccgtggt ctctcacccg atggctctgg cgcaggccgc gacctggctg cgccgcgagt
    27301 taccgaatgc cacgaccgaa gaggtcgagt ctacggcgga ggcggcgcgg cgggccgccg
    27361 aggagccggg tctcgcggcg gtcggcagcg tgctcgccgc cgaggctcac ggcctgaaca
    27421 tcctcgcccg caacatccag gacgcccgcg ccaacgcgac gcgcttcatc gtgctgggcc
    27481 gcgcctgggc ggggaggacc ggccgcgaca agaccagcgt agtgttctcg gtaaaggacc
    27541 ggccgggggt gttgaaggat gccctttcgg ctttcgcgga gaaaggcatc aacctcacca
    27601 ggatcgagag ccgcccgagc cgcaagcggg cctggaccta cgtgttcttc gcggacttcc
    27661 agggccaccc ggacgaggag cgagtccagc aggcgctcgc ggggctcgaa gagcactgtc
    27721 cctacgtcag cctcatcggg gcctaccccg aagtctcgcc agaggtcgtc ggctagcgcc
    27781 gggcgccgtg agcttcgtct ccgccgccag ggaggtgctt caggaggtcg gatatcctct
    27841 gcactactcg gacatcaccg agatagccct ggagtccggc tacctgagga gcagcgggcg
    27901 cacaccgcac aacacgatgc gcgcccggct ctccgtggac gtgcgggaca accccgagag
    27961 cccgttcatc cagacggctc cgggggtcta cagtttgaag cgcggcgact agagagcgga
    28021 ggcggatgct gtatattgaa ctgccggata gccgtgatcg gggaatcgtg atcggatggc
    28081 cggatcttga gacagtgagg agttcgcgtg gttgagcttg cggagcgggc cgtggacgcg
    28141 gcctacaaga taaagaacct tgaagactat gaccggaggc tgcggcgggc cgcaaggagg
    28201 gtagatgaga cctcgcgcgg gcatggcacg gcggcgctcg tgatctacga ggccgccttg
    28261 atgagcagcg gccgtacgat agacgaggtc cgggagttgg tggacgagtt tcaccggacc
    28321 tgggaggacg aggaggagcc gctcaccatc ccgcgcgtca gcggcatcat gaacgtccac
    28381 ggggacgagt ggttcttcgg ggactcgggt ctgcggagac tcgcagggcg cctgctcggg
    28441 cagttccagc acagggtcgc acagtacaac tacgtggacg agcgcgaggt gctcaagcgc
    28501 tgggccgact cgtacgatac ccggctcttt atccggcgcc gcatcctggg gacggagccg
    28561 gtcgtcgggg tcgtgggcgg cctggacctc cagagagtcc agtacctgcg cgtcatcgct
    28621 ggcggcaaca ccctcgtgcc gtcgcagggc gtggcccgcg ccctcgaagc ccttggtttc
    28681 gagacgaatg atgactacga aatcctcggg cacggggaga ctctggccct gcatatggac
    28741 ctgcccgcgc ccgtggtcgg agcgttgctc gaagacctcg cccgcgacga tgtgagggac
    28801 tttcccgcac ctccacggtc cgcggaggaa ccgggcgagg agggacctgg cgaggaggga
    28861 ccgggcgagg atacgggaac ccagaagccg gatgcggagc agcgagccgc cagacgcgcg
    28921 acggcgggtg atcccggaaa gggcgaggag ccgacgcgag tccaggaccc gcaggccagg
    28981 gacactcccg gagtgccgga ggagagcggt gaagccagca ggccagcatc acccaggtcg
    29041 gggaagggcg cggaacccgg cacctccgtc gctccagaag acaaggatgc ggagtagaaa
    29101 gagtcttgat ccgttcgccc cgcatctacc ggcaagcatg cgcagataac aggtctcttg
    29161 catagagccg gaagatgata gaatgccggc agcttaccaa tattcgctat atcaggcgtc
    29221 tcaatgagta ttgattcggg gatgggttca gccaagaggt aaacaaggag ctagcggaag
    29281 aatggcagag tcaaagaagc gcattgcttt aaagccacac atgagggaga tccgcgagtg
    29341 ggtagacgcg ggcaagacgg acgagtggat cgctgacgct ctggggacca gcgcctcgtc
    29401 cgtgcagtcg ttccgcagcc gcaacgacat ctaccgccgc gagaggggag gccgcgagcc
    29461 ggagagtgtc ttcgagggcg tcctggatca cggcgaccgc gacggatggg ggttgtggct
    29521 ggaccccgcg atcgccgagg atccgatctg gaaagaacac tgggccggag ttgacgcggt
    29581 gatagtgaaa atcgccgagg actctatcgt gctggagatg gacgaccggg ggagccagcc
    29641 ggaggagaac ggcctgagtg tcgcttctgt agtcccggcg ggcgccgagc ccgagaaaac
    29701 caacggcgcc gccagcgctc cgtccgccgc cacaccgcct gccacaccgc ctgccacagg
    29761 ggagactgaa gatctgcccg aggagcaggg ccgggttaag tggttcgacc ccgacaaggg
    29821 ctacgggttc ctggtgcgcc ctacgggcga ggacctgttc gtgcaccact ccgaggtcca
    29881 gggggacccc gcggcgttgg ctcccggtag cgacgttgag tacgaggtcg gcgagaacga
    29941 tcggggtccg aacgcgcgcc gtgtccggct cgtggactga cgccccggat agctccgcgt
    30001 cccggttcct ggccgctgct gccatcgggg ccgggacatt cttcgtatac ctgagcacgc
    30061 ttgcgcccac gatcgcgacc aacgacgccg ggcggttcca gatcgccgca ccaaccctcg
    30121 gcaccggcca cccgaccgga tacccgacct tcatcctcct cgggaagctc tttacgtacc
    30181 tgccgctcgg agatatcgcg tacagaatga accttatggc tgcggtcttc ggggcggcgg
    30241 cctcggtcct gtttttcctc gcgtcccgcg agatcggggg ccgcccgttg gcggcggccg
    30301 ggggcgctct cttgttcgct ttctcctcga cgttctggtc ccaggctacg gtggcagagg
    30361 tctacacaat gcacgcgacc ttcgtgctcg gcgtgctgta cctgctcctg cggtggcgcc
    30421 ggagcggggc tgccgcctcg ctgctcggcg cggccctgct gtacggagtc tctctgggaa
    30481 acaacgccgg gatggtgttg ctggccccgg cgttcgcggt cctggtgctg gccggacgat
    30541 ggcggaagct cacggcggct ctggtgggac tggcggcgct tgagtgcgcg ctgggactct
    30601 cggtttacgc ctacatcccg atccggggct tcgccggcgc ctggcacaac tacggagacc
    30661 cggtccacaa ctggggagac gtgtggaagc tcgtcacggg ggcgcgattt caggggctca
    30721 tgggcggttc cccggcgcaa ctactccagg gctcgggccg cttcctatat ggactcgcga
    30781 cgcaggcgcc gccgccaatc ggctacgcgc tgggcctcat cctgtttgtc ggcggcatct
    30841 tcggcgcccg cgcgctgtac atgagggata aggttgtggg cgtcgccttg atcctcgccc
    30901 ttaccgttac gctgttgtat gcgatcagct accggatagc cgatatcgcc gtctactaca
    30961 taccggtcta cctgttcctg gggctgtttc tgaccgtctg ggttacggat atcgccggtc
    31021 gagagggcgg acggagcgcg ctcgcggccg cgcccctctg tctggctgtt ctggcgctcg
    31081 cgttcaacta cgggcacgcg gacaggagcg actattacgc cgagcgtcag cggtccgagg
    31141 cgatcatggc gcggctgccg gaacgcgctg ttctctacgg caagctgccg atagtgccgg
    31201 tgacatatct aagagaggtc gagggcgtgc gcgaggacgt taccctgcgg tggctcgatg
    31261 gcggtaccca ggggcggcac atgtactccg atgtagagag cggccggccc gtgtacgtga
    31321 tctcggaccc gcgctacaac gaaatctacc tgaagtcggt caggtcttac gccaggaccg
    31381 tggacgagaa cggcctcatt cggcttgatc cccgataggt aacctcgccg cgctctcgtc
    31441 gggaaggtgc gcggtggatg cgagttccag caagcggggc ctcaagccgt tcgtgctcca
    31501 ctcgatgcgg gtgatcgagg tgttagggac gcgctgcgtg ccggggagcg cacccggacc
    31561 gaagatctct cgtaggaacg cgcgcatgct gccgccgtga gagacgacca gcactcgctc
    31621 ccccgcgccg tgacgctcca ggatctccgc catgcctctc tcgaagcgct ccaggacctc
    31681 ttcgtcggtc tccgcgccga cgagcgtcca ccgctcctcc tccgggatgc tgaggaacct
    31741 ctccatcaac tccggatttt cggcctctct ttgctcgacc gttgttcctt ccagagagcc
    31801 gccggcccgc tccatcaggt tcggaaccga aactacctcg ccggggaact gcgtctcgcg
    31861 ggcaatgatc tccgcggtct cgaacgcccg ggcgagcggg ctggagtaga tggcgtctat
    31921 ttgctcgccg gatagcgccc ggccggtgtg ccgcgcctgc ctccggccct cctctgagag
    31981 agggaattcc atctggccct gccagacgcc actggcgttc gccgtggact gtccgtggcg
    32041 gatgatcact atctctctac tctcgggcac ggcggtaagg ttagcatgac cggcgctttc
    32101 ggcaacctgt atcggatgct agaatcggcg cgtcgaagct aggggcgagt attcaggggg
    32161 tgtccagatg ttctggaagc gtaacgacgg agagacgcgg gaggaagaag agactcctca
    32221 gcggccgtcg ggaccgatcc agatcgaacg cgacgcgcag cggcccgcta ctatcttgcg
    32281 cgtcgcgggc gagttggagg agcgtggcgg caagatactg gagcttttta aagaggtgca
    32341 gacctccgat aagcagaccg ccgtcatccc catccacgcg gcccgcgacg gacgcgagtt
    32401 cttcgtagag gttgcgaccc ggccctggac cccggactcg gtagaatcca cgttgcgtat
    32461 ggtctccgtg gtacggaact ccgactatgc cgatacgccg cttgatatct tgagcgccta
    32521 cccggtcccg caagatatcg agttcttttt ctccgactcg ccggtagcgc tgttccagtt
    32581 ggagctgatg cgcggagacc aggcaaagcc ggagcagttc gcagagattt tccggcaggc
    32641 tgcgagccgc cactgggaca cggaactgga ctacgagacc tcctgcctac cgctggtcga
    32701 ggaactactg acctcggcgc tggacgcgga cgactcgggc gaacttccgc caatcctcaa
    32761 cgcgctggtc gagggactcg ggtgttttac gggagaggtg atccggcgcg agtccggtat
    32821 tcccgccgcg tgggagaccg ccgcaggttg gggagacagc ctcatcctcg cctttgacga
    32881 cctaacccta gactccgtcg ggaaggctcg cgccttcctg cacgaaggca aggaggactc
    32941 tatctccttc tacgccgagt acgcgctccg ggaactcaag cgcgccccta gcggcgcagt
    33001 ctagcgccct cgtccagcag gagcatcgtc atttagagct atctaggcgt agcgccggcg
    33061 cagttcccgg attatgtgcg tcgaatccac cagcacctcg tcaccgatgg tcaggacggg
    33121 gatggcgcgt tgctgcgaga gttggatgac cgtctcccgg ccgtcggcgt cggcgtttac
    33181 cgactcgtat tccaggccca gacggtccaa ttccttactc acccttcggc agtaggggca
    33241 atatgctccg gtgtaaagct ttatatcctg cgaactctct tgcgctacgc tcaaagcgat
    33301 cctctccatc tgttgataat acctacgatc aagagccgag attatactcc accgtctcgc
    33361 cgaacggccg cgaatctgca caagacagcg cacctcgcgg ccgagagcta cggcgatctc
    33421 cacaaattct aaggttcaac cgccggtaca tcctgactct gattctcagg tacaaaccgg
    33481 gatagaatat gacttccggt actggcgaaa gctcatgcgg cgtgctaggg ttgcagttcg
    33541 agaggggggc tctcttgaat agacgtgtcc ccgcacagag cgcggggacg gtagatctac
    33601 tgtacggagg aaaccggttg actctaaagg accggagaga gaagagtgat gcgagggaga
    33661 acacgctgag gaatttcctg ggcggctcgc gcaattccaa gaaaggcggc gggcggaatt
    33721 ttgccggtcc gatcatcatc gcctgcgccg ttatcgcgat gatagtcgct gccgactact
    33781 ggataaacgc cggtaagatc taccgcggcg tcgaggtcgg gaccatcgcc ctgggtggca
    33841 agactcccga ggaagccaag aagatcatca ccgaaagaac gacgggcgcg ctgctaaagg
    33901 agatcaagct ctccggacct caggacttct cgcgcagcgc ggcggatatg ggcgtggatt
    33961 tcaatgtttc aaggaccgtg gatcaggcgt acgccgtcgg cagggagggg aacctcctcg
    34021 accggctggg tgagcggttg aaggccgctt acgggaccgt caccattccg cccgacgtga
    34081 actatcagcc ggagaaggct cgcgtacagg tggagaacct cgcggcgcgc ctcgacgaga
    34141 aaccgaagga cgcgacgatg gacatcgttg gccccgaggt ggagatcgtc ggttcgaagg
    34201 agggttacaa gacggacgtc gcggcgacca tgaagagcgt gaacggcgcc gtcgaagaca
    34261 tgaccggcga ggcgaagatc gtcggggagg tcctcaagcc gcaggtcgga accagtgccg
    34321 cgaaggaggc cggcgagaag gctcgcaagg ccatgtcctc gcagatggtg gttacctcgg
    34381 gcaagcagaa gtggacggtc acgccgtctg cgatagggga cgcgctggat gtggagaaga
    34441 agggcggcgg gatccaggtg agcctcaacc gggatcgtat gcgcaataac ctcgccaacg
    34501 tctatgccgc gctgaccgta aagccggtcg aggccaacta cgtagtcaac ggctccaagg
    34561 tcacggtcca gccgggtcag accggcgtga gcgtcgagga caagaagttt atcggggcga
    34621 tcgaggacgg aatcttcgac ggcaagcata gctatacggt accggtcacg aaggacgagc
    34681 cgaacctcac gaccaccgag gcagagaagc tcaagccgac ggatcttctc agctcgtatc
    34741 agacgaacta cctcacctac gacgatagcc ccgggcgggt gacgaacctg gagaccgcgt
    34801 ctggcgccgt gaacggcacg ctgctggccc ccggcgaggt cttctccttc aacgcgctcg
    34861 ccgagccgct tcaatacgag aagacgaagg tcatcataaa gggcaaggta gacgaggcgg
    34921 agggcggcgg cctgtgccag gtgtcctcca cgctgtacat ggcggccaac ctggccgggc
    34981 tcgacgtcat cgaacgtcac ccgcactacg ccgagctgcc gtacatccag cccggcttcg
    35041 acgcgaccgt ctggtttggg gcgctcgata tgcagttcaa gaacacttcg cccggctacg
    35101 tgctcataca ggagtgggtg gatgaccagg gttacgtcaa cgccaacatc tacggaaagc
    35161 ccagtggggt caaggtagat atggactctc agaaggtcag cgagtctacg gacagcgagg
    35221 gcaaccccgt gaccaagtgg gtcacctacc agaagattac gaagaacggc aaggtcgtct
    35281 tcgatgacgt gctacacgag gacacttaca agttcctaaa gcccgccgaa gaagacaatg
    35341 ccacctccgc cgaggtgcct agtactcagt aaagagtaga gcggtctagt tctaattttc
    35401 tacaggttgt gcggctctcc gtactggagc gcgggggaag actagccggt ctcgacgacg
    35461 atctccactt cgaccggcga gtcgagcgga agctccgcaa cgccgatggc ggagcgggca
    35521 tgcagtccgg cctccccgaa gacctctccg atgaagtccg aagctccgtt cagcacctgc
    35581 ggctgggcgt tgaatccggg ggcggaggct acgaagcccg taaccttcac cacgcgacgg
    35641 atgctgtcga gaccaccagt ctccgaagcg gcggcggcca gcgcgttcag cgcgcagagg
    35701 cgggcggcct cggtggcttc ttcggcgctg accacgtctc cgacttttcc ggtctttcgc
    35761 aactcgccgt tctcgaaggg gagctgtccg gccgtgaata cgaggttccc ggactttgcg
    35821 gcgggcaggt aggagcccgc gggagcgggt acggcgggca gatcgtagcc cagttcgcgc
    35881 agccgttcgg aaggggaggc catctatcta agtgcctcct cgtactcggt cgggtcgaag
    35941 atcagggggt agctctcgtg gagcgtgtcc ccggcggtta cctccccgat tatcaccgtg
    36001 tggtcaccgg cgcgggcggt gtccatgacc cggcagtcca gataggccag acagtccagg
    36061 aggaagggcg aaccgccggg cgtgagaccg tagggcactc cgaggagctt gtcgtggtac
    36121 tcgccgctgg tctctgagaa atgggtggcg acgtctacct ggtcctcgcg cagcacgctc
    36181 aggccgaatg tacccgtctc caggaccagg tcgcggctgt agcggtcgtc ccggacggcg
    36241 actgccacgc tgggcggccg ttcggagacc tgcattaccc acgaggcggt catcgcgttc
    36301 gactgacgac cgcggcgcgt gccgagtaca tagacgccgt gtgtcagatg gcgcatcgcg
    36361 gccagtatgg agttgcggtc ctgtggctcc ctgtccaccg tcctccgttc ggttctggga
    36421 ctggctcctt gtgtggctcc gtacgtatgt agtttagcag ggtagagcgg gtatactgcg
    36481 cttcatgttc gcagggggag atccagaagc atgagcggcg cgctggtcgc tatcgggatg
    36541 gcgcaggctg tcgtgtacct gctcttcatc cgcttcgtag acttgtacga gcgcgagccg
    36601 ctttggtacg tgatccccgt ctttatctgg ggttttgccg tggctaccac cgcttccctg
    36661 ttctttaaca ccatcgccgc cattaccatc agcaccatcg ccagcacgca ggcggcggac
    36721 actctgacgg cgatcttcgt ggcgcccgtc gtggaggaga gcttcaaggg gttggccctc
    36781 atcatcgtgt tcctcgtggc gttgatgctg cggcggagag gagcgatgga gtactccggg
    36841 gtgatggacg ggatcgtgta cggctccgcg gtcggtttcg gcttcgccat agccgaggac
    36901 ctgatctact acgcccagta cggggcggag gtcttcgttg tccggcggct cttcggcgga
    36961 ttcgcgcacg ccgcgttcac ctcgctgacc ggcgtgggga tcggcctgat accgtgggtc
    37021 cgtagcggtc tcctgaaggt cgcgctgccg ctggtggggc tcgtatgcgc gatgctcttg
    37081 cacgcgacct tcaacttcac ggcgacagtc ttcgggtcag tggcttacct gctactcctg
    37141 tgcgtgatcg cggcttacct cgtcctgatc gcgatctggc tggccgtcga gcggcgcacg
    37201 atccgggacg aactgcgcga tgaggtcggt ttcgggacca tcagccagga agagtacggt
    37261 atcctgccga cctatttccg gcgcaccgcc tactacctgg ggttgatgtt gcgggggcga
    37321 ttcggcacct ggcgcacggc caggaaggtc cataacgccg ccgtggacct ggcctttacc
    37381 aagcggctca cccgccgctc gtggtctgca tccggtgaga gccgggtgga cgagctgcgg
    37441 gggcgcatcc gcgcgctgcg gggaccggcc gccgcgatgc ggacctgaac ccgccggagt
    37501 tggcgtcggg gatcatcctg gccggcggcg caagcagccg gatggggcgc gacaagctct
    37561 ccctcactgt cggcggcgtc ccgctcatcc gccgtgtcta cgacgttcta tcctctcact
    37621 gcgctgagct gctggtggcc ggcgcggccg acaggctgcc ggagattccg gtcgcgcgga
    37681 ccgtcgagga cctgcgcccc ggacgagagg gacccctggc cggtatcgaa acggggctga
    37741 cggcggcgag gtattcgctg gtcttcgtcg ccgccgcgga cctgccgttc ctcccggcgg
    37801 acctcgtggg atactccctg acgctgctca ctcgtgagcg tgtgagcgtc gtagcgccgc
    37861 gccacgaggg attgcatcct ctgtgcgccg cctacgaccg cgcgatactc ccgcaggtca
    37921 gcgcggcgct cgatactggg gagcgctccg tgacgaggtt cattcgcggt ctgagggggg
    37981 tccggttcgt ggaaggagaa gagttgagcc ggttcgggga gccggaattt ttcctgatga
    38041 acgtcaactc gccggaggac ctggagcggg cgcgagcctg cgggggcgtg gagtgaggct
    38101 gccttctcct cgggagatcc tcggagacgc ccggacgctg gtgaacatct atcgcggcgg
    38161 cgaggtcccg ttccgctacg acgagcgggc tggagaagtg cggacggcgg ttatcctggg
    38221 agcgcaggtg ctgcggggag ggagagccag ccggacgctg gaggctcggg tgcggcacgg
    38281 cgcggagctg taccagcgtg gggcagttga cctgttgatc ccgaccggag gtctgggcga
    38341 gcacccgccg agggaggcgg agatcatggc gtggttactg cgagacgcag gcgtgccgga
    38401 gagcgcgata ctcctcgaag accgggcgac gaacacctgg gagtcggccc tattcgttgc
    38461 ggagatggag cgccaggaga gtataggcga gattcgggcg gtgacggacc cgctgcactg
    38521 tccacggacg atccgggcgt tccgggaggc cggtctccgg gcgtgggcgg agccggtgta
    38581 tagtagcccg atgtggcgcg tgccgttgat gcgccgagga cagctcgcta gagagatggt
    38641 ggcgtcggtt tggtacaggg tgaggcacgg gatcggctcg cgctccacgc tatagcggtc
    38701 ggggtgctgg cgatctccgc ggcggcgatc ttcatccggc tcgccgatgc tccggcgctc
    38761 gcggtcgcgt tctggcgctc ggccctgggc gcggtggtgc ttcttcctat agcggttgcg
    38821 cggcgggaga aactgccgga gccgcgggta ctggggtggc tcgtgctctc cggggtgctt
    38881 ctaggggctc atttcgggtt ctggatctcc tccctcgact acacgagcgt cgcggcgagc
    38941 gtggtcctcg tctgcacggc gccggtcttc gtggcgatcc tggcctacct gacgattggt
    39001 gagaagacct cgctggtctc gttcgcgggc atcctggtcg ccgtggtcgg cgcggtcctg
    39061 ataggtggag atcgctcggt cggttcggcg gcgttgtacg gtaacgtgct ggcgctgatc
    39121 gggactgtaa cgatagcggt ccacctgctg atcgggcgcc ggacgcgcac cggggggctc
    39181 ggcgtgctcc cgtactcgat aatcgtctac tccgccgccg cgctcacgct gctgcccgcc
    39241 gcgctcatca ctggcgagcg cctctggggc tactcgggag agacgtggtt ctggctcttc
    39301 acgataacgc tgggaccgca gatcatgggc cacactgtct tcaactgggc cctgcgctac
    39361 gtcgaggcgt cggtcatctc ggccacggtc ctcgccgagc cggtggccgc cgcggtcctg
    39421 gcgtggttga tcctctccga acgccccggc ctcgccaccg ctatcggcgg cgccatagtc
    39481 ctcatcggcc tctaccttct gatccgcgga cgccgcaacc cggcggcgaa gacggccacg
    39541 aagcgggtct gaccgtctcg cggaccctac aaaacctctc acaaaccgta ccggcgcgat
    39601 gatgtatgct gcgtttcgat gtgaatgact tatcttctgg agagaccttt gggaccgctt
    39661 aggaccgggt tcgtgtacgg gctgatagct gtacttctga cgctcgtggc cgggttccgg
    39721 ttcgtgtacc tggacccggc cggtacgccg gagtgggtgc tggcgaccac ggagacattc
    39781 ctgccgctgc tggtggcggc ggcccagatc ttcctgggca tcctcgccgc gatccggacg
    39841 ctgcctgtcc gcgtggaccc ggatgtgcct taccgcgcca tactcgtgcg cgactgtacc
    39901 ctggcggcca cggtcgtcgc cgtgatggtg ggtgtggctc taatactgat gaccgccctg
    39961 cacgcgacgg tcttcgccga cgacctgcgg gcctacgctc acgacgccgc cccgcgcatc
    40021 gccgcctacg tgaacgagac cgggaaagac ttgcgggagc ctccgccgcc gacgagcgcc
    40081 ggacagatag agaagaacct ccaacctccc gtcgtcgagg atctcggacg ctcgctgttc
    40141 aacctggtgc tgcgggcgtt actcatcggc gcgttcggag cgggggtcgg ctttatacgg
    40201 agcttctccc acagccgccg ggcgagtgcc gccgcgcaag ggaagccaga ggctggaaag
    40261 tagctgtagt gggatgacgg accgcgcggt gcaggtcgcg gtggccctgc tcctcgacgc
    40321 ccacgccggc gagcctcccg ccagagcgca cccgacagtc tggatgggtc gcgccatatc
    40381 ggctttcgag aagcgtgctc taggcctcaa gagtcccaga gatcggctcg tggcgggagc
    40441 cgccttcgcc ttcgccctgc ccgcagtcgt ttttatcgcg acacgcgcgg cgctctcgct
    40501 cgcgccgcgg cccctgcgca ttacgttgga ggtcgtcctg ctctcgacga ccctctcgat
    40561 gcgcgggctg gctgacgcgg ctctcgcggt cgaacacgaa ttggagagag gagacctgga
    40621 gagcgcccgt gcccgcgtcg gagagttcgt tggccgggac actgcgacgc tctccgaatc
    40681 cgaggtctcg cgcgccgccg tggagtcggt ggcggagaac acgagcgacg gcgtggtcgc
    40741 cccgatgttc tacggcgcct ttttcggggc gcccggcgcg ctggcctaca aggccgtcaa
    40801 caccctcgac tcgatgctgg gataccggga cccaccgtat acgcacctcg gccgggcctc
    40861 cgcccgtctg gacgacctcg ctaacttcct gcccgccagg gtaacggtcc tcgccgtagc
    40921 cgccctggcc ggcccggagg ccgcccgtgc cgcatggcgc tacggaccgc tgaccagcag
    40981 cccgaacgcg ggatgggtcg aggccgcgtt cgcgggtgcg ctcggggtgc ggctcggcgg
    41041 cgccaacgcg tacggcggtg tgatccaccc cggcgccatc ctcggtacgg gccgatcacc
    41101 cggaggaagg gacatagccg cggcggttac cctgatgcgc cgggtctgcg cgctcctggc
    41161 ctgcatggcc gtcttggctg gaggcatcag gcgtggttag gggctgggcg gggcatgcgg
    41221 acgggcgtca ccacggcgcg cacggcgcag aggtattgcg tgccctgggt ggaacctcca
    41281 atgatttcct ggattttagc cagaacatca acccgctcgg ggcgcccgcc agagccgtcg
    41341 aggccgcccg gaatgccggt ttcgccgtct accccgacct ggggtacgcg gatcttcgga
    41401 aggctctggg agagtatgtg ggcgtctctg atgattacgt gctgcctacc aacggtggcg
    41461 cggaagcgct ctttctggct gcccgtgcgg cagccgaggc gcggtccggg aagaaggccg
    41521 ttatcctgga gccgacgttc agcgagtacg ccgcagccgc ccgcgccgcc gggttcgagg
    41581 tcgagcgccg cgtggcccgg cgggcagagg atgccttcac gatggacccg gagaccctcg
    41641 gagatctggc cgatgtcggg cttgtgttcc tgtgcaaccc gaacaacccg actggcgggg
    41701 cgatgacccg ggatgcgatg ttagaggtgt cgcgttgtct ggcggaagca ggagtcgtgc
    41761 tcgtcgtgga cgaggcgttc gcggacttcg tgccgggggt tagcgtcgct tctagggtgg
    41821 gactcggcgg tttcgagaac cttctggtcg cgcggtcgtt taccaagttc ttcggcatcc
    41881 ccggtttacg gcttggagcc ctgatctcag ccgacaccgg cctcgccgga gcgttccagc
    41941 cgtcgtggtc tgtaaacgcc gtggccgccg ccgccggcat cgccgccgcc tcagaccagg
    42001 ctttcacgaa gtcttccatc tcggaggtga cctggctgcg cgaggacctc tacgctgagc
    42061 tggaggcatt gccggggatc cggccgtatc cgggagcggc gaacttcctg ctggtagagg
    42121 ggccggatac cctgacggag cgactggctc gccgccgggt gctggtgcgc agttgtgagc
    42181 cgttcttggg attgggaacg ggttacttcc gagtggcggt tcggacgatg gaagagaatc
    42241 gacttctcgt cgaggcgata agggaggagc tgtaaccgtc cgcgctctat tcctgggcgg
    42301 ctcccgcagc gggaagagcg ccgccgccga gcgcatggtc cacgatctgg gtgcggatcg
    42361 cgttctgtac gtggcgaccg ccgagacttc cggcgacccg gacctggagc ttcgcgtgag
    42421 ggcgcaccgc gaacgccgtc cttcggactg ggagacgctg gagcttggcg gcggcgatct
    42481 gaaggcggcg ctggacgccg cctataggca cgaagccgtc ctcttcgact cgctgacgct
    42541 gtgggtctcc gcccggatgg aacgggagaa ggagacgctg gctgagttcg atgctttcct
    42601 cgaaggagcg gcgtctatgg aggcggacgt cgttctggtg agcgatgagg tggggctcgg
    42661 ggtggtgccg gagagcgcgg cgggccggag gtttcgggac ctcctggggc tcgtgaacca
    42721 gagagccgcc gcctcagccg gagaggtaca tctctgggtc gcgggcattg gcgtgaggat
    42781 caaataacgg tgcgcgcgct gctcgccttc tttaccgtgc tgccgctgcg tggtgcgacg
    42841 ctgcgggaag ccgcgcagag cgtacacctg ctgccgctcg tcgggctggt caccggtctg
    42901 cccggcgccg cggcactcct tctcggatat gcgctgccgc ccggtgtcgc cgccacgctc
    42961 gggataggag cggtgctgct ggccgccggg ctgcaccaca gcgacggtgt cctcgacgtg
    43021 ggcgacgcac tcatggtccg aggatcgcgg gagcgccggc aggaggtcct caaggacgcg
    43081 agggtcggga tcggcggcct cggggcgctg tttctggtct acgctcccgc gcttaccgcg
    43141 ctcgccgcta tagcggacac ctctccggta cgcgccgctc tggcccttct cgctgcggag
    43201 gtggctgttc gctcgctgat ggtcctcgtg ctcgcctttg gcgagcccgc gcgagccaca
    43261 tcctccgcgg tcgagtttgt gcgatctctg agcggacctc ggcggacggc gggcatcgta
    43321 ctggccctcg ccgcgccgct actgctcgca ctgccgctcg gcgctgctgc gctgctggcc
    43381 gctctcaccg ttcctctagt tgcgttgttg gcgttgcggg tgtctggata cgccttcggc
    43441 gggatctccg gtgatgtctc cggcgcgacc ggcgagttgg cccgcgcggt gttcctggtg
    43501 gtgctgtcgg caacgattta gccccccggc atggttgccg gagggccaaa tcgctactga
    43561 ataagactag cggctaggag gcgaggttgt cgcccggctg catgacgtga acggtgactt
    43621 ccggggcgat ctcgtccagg tgcttctgga actcttccgg ggtgccggga aggaacggga
    43681 aggtcccgta gtgtatcggg acgacgtggc gcactccgag cagacgcgcc gcgtgggcgg
    43741 cctcgaacgg gctcatgacg acctggtttc cgatggggag aagcgccagg tccgggctgt
    43801 atagctcgcc gatcagggcc atgtcgccaa agacggcggt gtcgcccgag tggtagagct
    43861 tgaagccgct ctcgaactct atgacgaagc ccatcggctc gccggcgtag atgctgctcc
    43921 cgtcctcggt ctggatgctg gaggagtgga aagcgttgac ggccgtgacc tttacgccgc
    43981 cgacctgggc ggtaccgccc ttgttgaggg gcatggcgtt ctcgacgccc tggctctgaa
    44041 ggtaggcgaa gatctcgaag ttagccacca ccgtagcgcc ggtgctctgg gcgaggggta
    44101 tggcgtcggc gaagtggtcg aagtgtccgt gagtgatcag gatcgtgtcc gcctccccga
    44161 cctgcttgag ctcctccggg gtctggggat tgtcggtgag gaaggggtct ataatgatct
    44221 gctctccacc ggccgtaacg aaccggaagg tcgcgtgacc gaggtagttg atcctggttc
    44281 cgccgagcat ctcttccacg atttcctcca tcttcttgag tgcagttgct tacgaaaaac
    44341 agcttacccc atacccggcg cccatgccgt ggaaacgatc ttccgccgcg ctgcctatcg
    44401 gatagtctat aaaaccagta agctgagagc ccaggataga gagccgaccg ctagggggtt
    44461 tgcgtgggat accttccgat agaggatcat ggcgtaatag gcgacctgca cacggcggcg
    44521 ctcgtcggta cggacggtac tatcgactgg ctgtgcctgc cggcctttga ttctccgagc
    44581 gtcttctgct ccatcctgga cgacgagaag ggcgggcact tcaccctgcg gcccacggat
    44641 tacgtccgca gccagcaact ctacctgcca gacacaaatg tgttgctcac gcgctttctc
    44701 tctcctcaag gggttgcgga ggtgctggac ttcatgccga tccacaccgg ccacggggag
    44761 cggcaccggc tggtgcggaa cgcgcacgtc atccggggcg agatgacctt tgaggtcgag
    44821 tgccggcccg ctttcgacta cgcccgctgc ccccacaagg tctccgtcgg taagaccggc
    44881 gtggtcttcg attgcggcaa cttccgcctc ggtctggcga gcgacaccgc tctaaaggag
    44941 acggacggag ggggagccgg cggcaggttc acgctcgaag cgggggagcg ggcgacgttc
    45001 gtgctggccg acatagccga gggagaggga ccgggagaca tcctctcccc gccggagttc
    45061 gaggagttgc tgggtcgcac gctctcgtac tggcggcgtt ggatctcgcg cagcacctat
    45121 caggggcaat ggcgcgaagt cgtgaaccgc tcggcgctgg ctctcaagct catggtctac
    45181 gatccgaccg gggctctcgt ggccgccccg acgatgggct tgccagagag gatcgggggc
    45241 gagcgcaact gggactaccg ctacacctgg ctgcgagacg ccgcgttcac gctctacgcc
    45301 ctcatggctg tagggttcga ggaggaggcc ggcaacttca tgggctggct ccgggaccgc
    45361 tgcacccagg acagcgacgg cctcctgcag ccgctctacg gtatagacgg acgcctggat
    45421 atagaggagt cggtcctgga tcacctctcg ggttacatga actccggccc ggtgcgtctc
    45481 ggcaacgccg cctaccagca ggtacagctc gacctctacg gggccgtcct cgacgctgcc
    45541 tacctctaca acaagcacgg cgcgccgctc gactacgacc tctggcagaa cgtccgcaag
    45601 atactggact ggctctcgga gaactggcag cggccggatg aagggatgtg ggaagtccgc
    45661 ggcggccagc ggcagttcgt ctcctccaag ctgatgtgct gggtcgccct cgaacgcggg
    45721 acccgcatcg cccgtcagcg cggcctgcct ggaggggatg gacattggat cctcgagcgc
    45781 gaccgcatct acgaggacat catgcagaaa ggctggaacc cggagcgcaa gagcttcgtc
    45841 cagtactacg gctccgatgc cctcgacgcc tcgctgctct tgatgccgct cgtgaagttc
    45901 gtcggtccca aggaccctcg ctggctcgcc accctcgaac gcatccagga ggagttgacc
    45961 cacgacaccc tcgtggaccg ctacctgaac gccgaggccg ccccggacgg actcgaaggc
    46021 gacgagggga gcttcagcat ctgctccttc tggctagtcg agtgcctcgc ccgcgccggc
    46081 cggctggacg aggcgcgact ctcgctggag aagatgttct cctacgccaa ccacctcggg
    46141 ctctatgccg aggaggtcgg tacctccggt gaggcgctag ggaacttccc ccaggccttc
    46201 actcacctct ccctgatcag cgccgccgta cacctcgacc gcgccctgca ccactcctag
    46261 accttgcgag ccccgctctt cgaggccgtc ccgaacttca gcgaaggccg cgacgcggcc
    46321 aggatctccc gcatcgtctc ttccgtccgc gccgttcccg gcatccgcgt cctcgacctg
    46381 cacactgacc cggaccacaa ccggagcgta ctcaccttcg ctggtgagga ggagcccatt
    46441 ctccgggccg ccgtaacact cgccgccgcc tgtgccgccg agatcgacct ctccgcccag
    46501 gccggcgttc atccgaggat gggttctctg gacgtgctcc cgttcgtccc gctcgaaaac
    46561 gccacgatgg acggcgcgat ccggctggcc cgcctcgcgg gagagagcat tggctctctg
    46621 ggatactccg tcttcctcta cgagcgcgcc gcgacctctg agcaccgtcg aaacctggct
    46681 gaggtgcgcc gtggcggata cgagggcctc accgagcgca taaaggaccc cctctggcac
    46741 ccggactacg gcccgaatgc tctcgacccg cgcctcggag ccgtcgccgt cggcgctcgc
    46801 gacttcctca tcgcgttcaa cgcctacctc gacacggcga acgtggagat cgcccgtcag
    46861 gtcgcccgga aggtccggga gcgggacggc gggctgccgg gattgaaagc gctcggcctg
    46921 gaggtcgcgg gcagggcgca agtctcgatg aacgtaaccg atatcgaggc tgcgccgctg
    46981 ccggtcgtcc tggaggccgt gcgcgcggcg gcggcggctc tcggcgcgcg gatcgagtgg
    47041 acggagctag tcgggctaat gccgctggag gccctgctcc agacggcgcg gcaccatctg
    47101 gcgttgcggg acttcgacgg gagacacgtc ctggaggcgg gtttgtggag atcggaagat
    47161 ggctgaagca acgtttcagc cagagtgcgc ggcccaggat acgccattgg cggtccggat
    47221 tctgatagaa cattacgcga acgggggccg tgcgggccgc gtgacgagcc tggaactttg
    47281 aacggaggaa tcggcgtgga gagtggaagg aagctggtag cggcggcgat acagatgtct
    47341 tcgacgccgg ataaggacga gaacatggcg acagccggag ctctgatccg gagcgctgcg
    47401 gcggccggcg ctgagttgat cgctttaccg gagctgtgga gttgtcacgg tctggataag
    47461 gtctaccggg agaacgcgga gcagattcca ggtccgacga cggactttct cggggagcta
    47521 gcgcgggagc ttggcgtcta cgtcctggga ggttcgatcc tggagggcgc ttccgggtcg
    47581 gggcggctcc acaatacctc cacgttcttc gggccggacg gcgagctgtc ggccgtctac
    47641 aggaagatcc accttttcga cgtgaaagcc ccggatcgcg agtacctgga gagcaagacc
    47701 atcgtgccgg gcgaggagat cgtgacggcg aaggccggaa cggccacgct cggtctctcg
    47761 gtctgctacg acgtgcgctt cccggagctc tatcgcctgc tcgctctgcg cggcgcggag
    47821 atcctggctg tgccggcggc ctttacattg cagaccggca aggatcactg ggaggtcctg
    47881 ctgcgggcgc gggccatcga gaaccaggcg ttcgtgatcg cgcccgccca gtggggccag
    47941 aaggcggacg gccgctggac ttacggacgg tccatgatcg ttgacccttg gggaactatc
    48001 ctcgcgcagt gccccgaccg ggacggctac gccctcgcta ccctcgacct cgactacctc
    48061 gaccgcttcc gcactgagtt cccagcgctc aagaaccgcc ggttggcgga tcagggctgg
    48121 tagccgcgct cctctgaggc gttagtggag ttggtagacg aggccgatcc aggccagaat
    48181 cgccagagcg agcgcgaccg gagccgctgc ggcccgcgcc agatctccct cgcgtccgag
    48241 cagccccgtg gccgtggcgg cgagcactag acgctgcggg gcgagcatgc tggcatggct
    48301 tccagccacg ttctgcacgg cggccgtgag cgtctccggt aggccgagtc cgcgggccgc
    48361 ctcgatctgg agcggcatga agagagcgtt ggaggccgcg ttggaacccg tgagcgcgcc
    48421 gccgagaccg gccagcacgg gggagaaggc cagatagtag ttcccgagac caccggcggc
    48481 ggatgcgaga agcgaagccg ctccgctccc ggcgacgact tctccggcca ggacgaaagt
    48541 caggacggca ccggccgtcg ggagccactg ggccgctgtt tcgcacaaag cctttccagc
    48601 agtgcccgca ccgactttga gcgtaacagc agcgaacgcc gacgacatca ggagtggcag
    48661 tcccggtccc gtgaagaccg ggccgagtct gcccaacgct gccgcgatag taccgatgcc
    48721 gttggctgcg gcgagcaggg cgagcaagaa cccgtagggt agaagctccc gcacgggcac
    48781 gcccgccgct ctctctcgcc tgcgtaccag aaagaacgcg gtcgcggcga gacctccgac
    48841 agcgccggag agctccggga cgaggaagag gctcgtgacg agcgtagcga caccggtaac
    48901 gagaccgagc agggcggctt cggcaccgtg acggcgcagt ccggcccacc ctccggcaag
    48961 cgcgacggct gctagaccgt atacggggaa gagcgggaga ctcaggtatg cgctcatgtc
    49021 ggagaggtcc tggaagctcg tgtccgcgag gttggccccg atgacggtgc cgaccccgag
    49081 cgcgccccac ggcaccgcgc attgacccca actggcaagc accgccgcct tcaggggtgg
    49141 gaagccggcg gcgagcagta tcggggcgct gatgaccacc gccaccccga aacccgtcac
    49201 cgactcgaag aacggcgcca caccaactac gaccccagca gccaacgcct cgcgctccgg
    49261 ctccaacctc ccgaggaacc gcgatacttc gtctgtcgcc ccaccagcgc ggagcaggtt
    49321 gtagaggagc agaccgccga agaggacgta gagtacctgg gcagacgttc cgagtccatc
    49381 cagtaacgaa ctgcccagcc ccgaagcatg cagcgaaggc cagagtagcg ccccgccaac
    49441 cgccgacgcg agagctgcta cccccgccca catggcgggc caacgcagca agatcagagc
    49501 caccaccgca accacgaacg gcgataccgc cacacttatc ccaataattt ctatcatggt
    49561 agaattactt ctaccagatg gacgaaggtg gcgcaagaga ggtcgggacg gtggtcgagg
    49621 cggtctcatc agagcagttg ggggctcggg tgcgggatct ccggcggcgg cgcgggttga
    49681 ctctggaggc gctcgcggag agttctgggg tgagtcgggc catgatctcc aagctcgaac
    49741 gcggtgagaa gaacccgacc ctcgtcatcg ccgccagact cgcggagggg ctcggcgtca
    49801 ccctctccca actcgccggg atggaggagc ggcgcgaggt gatcgtggtt cccagggaca
    49861 ggcgcatggt catgcgcgac cccgagacgg gtttcgagcg ccaggtactc tccccgaact
    49921 tcgccgggcg aggcgtggag ttcgtccgca acgtagtccc ggagggaggg acgtcgggtg
    49981 agtttccgcc acacaagaag ggcgtagagg agtacatcgt agtggaacat ggccggctca
    50041 gggcggtgct tggagccgag gagtatgtgc tggaagaggg cgacgccgtc tacttcgagg
    50101 cagatgttgc gcaccgcttc gacaacctgg gcgagggagc gtgcagctac tacctcatca
    50161 taagctcacg aggtgtgtga gccccggaga ggtcgcgtta tgaggcttcg agcaggctgg
    50221 tcttgcggag gcgagcctcg tagccggcgc ggtgtccgcg ataatattcg agcctttcgg
    50281 gggcgttctc ccacttcatc aggctgcggt tcctggtgaa aagctcctgg ctcctgtagc
    50341 ggccgtcgag ccacccggac cagtaggcct cgctgtgatc cggcgacttc cacgagactt
    50401 tgcttgcgga gaccggtacg acctcggagc cgcaggccgg acaccagtag acgccgtccg
    50461 ggagacgccg catctcgggg tgtccgcact cgcaggcgtg gttccccgtg gcatccggca
    50521 gcgacgctat atccaggagc gtcttgagcg tcgggccgcc tatgaggctg ctacaggtgt
    50581 cgcagcgggc ggactcgcgc ccgacgggat gaaggacgcc cgatccgcac agggggcagt
    50641 tgagggtctt cgggctggtg gttgtcgaga ggtctgccat ctcctgtcct ttcctggtat
    50701 ctcttgtctg taccagtaat tatccgtccg aaggacaagg aagacatcgc acaaatgatt
    50761 gatcttttta tcacgagaat taagcagtag tttctagctc aaatggcgta gcccatctgg
    50821 tttaggctct ttagagcgtg aaacgccccg tcgagagctg ggccaggacc cgttcccgca
    50881 tctccgagac gtccgagccg gcagcgtaca ggtcttctat gggttgcagc aggtccgacg
    50941 cgcccagagg gatgtccgcg ccggcggggg tgacgtgatg ggagccgtta tccagccgga
    51001 gtagctgctt gcgtccgccg cgtttgccgc gtttggagcg gtcctcgccg ttcacctcca
    51061 cgatatcgag ggagtagtcg atcatggggg catccgcgat ggcgcccccg accccgtagg
    51121 cgtcgcagac ggggttcagg tggaggatgt agtccacgtc aatgccgccg gaggcgaaga
    51181 tcttgacgtg ggagaagccg ttgcggtcga gctcccagcg cacctcgcgc atcaggtcgc
    51241 ggaagtcgcc gcgccggttg ccgggggtgt ccatgcggat ggcgtagatg gagtccggga
    51301 tctcccgcgc cgctatgagc gccccgaact tctcatcgtc gaaagtgtct atgaggatgg
    51361 tgcgcggtac gtccggcgcc acggtctcgt cgaaggcgcg ggcggcctcg gcggtcgagc
    51421 cgatcaggag caccagcgcg tgtggcaggg ttccgctggc cggaattccg agcctgcggg
    51481 cggcgagctc caccgctacc ccgtcgcagc cgcccaggta cgcggcccgc tctatcatcg
    51541 gcgtgaccgc cgggtgcatc cggcgcgccc cgaaggagac gaccggctta tcgccagcgg
    51601 cgatgcggca ccgggccgcg cccgtggcga cccccgacgc ctggcaaaga aagcccagga
    51661 tcgccgtctc gtgctccgcg aacgcgccgt acggaccgga gagcgcaagc accggctcgt
    51721 tcggcccgca gatggtgccc tccggcaccg cccgcacctc cacctccgtt ccgtccaaca
    51781 ggtgcgccac ctcgtctagg cccgccacca cgaaccactc gtccgggttc gaggtcttgt
    51841 aggcgacctc cgcgtggacg agagcgttat ccaatccgcg ggcgcgcaag acctgcatcg
    51901 tgcggtggaa gtaaacgtca gccacctcgg cgccccgtat ctctgtctcc gacgccgtgt
    51961 tgaacctcaa gctcccgata ccgccgctct ttccccgcaa ccgcgtgccc tttccactag
    52021 cctgcctgcg gcgtatcata gctaatcgaa ggacttccga tctcatgtcc atcgagacat
    52081 gctgttttgc aggtagaatc cgctgcaagg gagggcagag gagtattagg ggcttgaagt
    52141 ctgcttccct gtgagccggc ggttctgtgg ctacgagtca ccctcttatc agccggtccg
    52201 accaacaagc ctcttctcgc ctaccaggcg ccttatctcg gctatgacct ctgagatcga
    52261 ggctgatttc ttgccgagta gctcgttggc tcccattctt attgcccagg cgcgaaactc
    52321 ggtactgtca aggacggtca gtaccagcac cggcaggttt gggttgactt cacgaagatc
    52381 ctcgatcaga tccgtgccaa caccgtcggg taatagcaga cctatgatcg cgacgtcggc
    52441 agccctccac cgcccgggga ctccgttgcg agcctcggcc agagacccgg cctgcccgat
    52501 cacctcaagg tccggttccc gctccagtgc gcgcgcaagg gcttgacgga agcaagcctg
    52561 ctcctcaagg agaaagatat gtttcatg
//
LOCUS       DASSMB010000030         5284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1146580, whole genome shotgun sequence.
ACCESSION   DASSMB010000030 DASSMB010000000
VERSION     DASSMB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5284
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1146580"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(393..950)
                     /locus_tag="VFJ72_02360"
     CDS             complement(393..950)
                     /locus_tag="VFJ72_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478339.1"
                     /translation="MEEKRKLHQLEVQFQMDSVEILDMVLSNNRLHIAVKGAVAQEHL
                     RKYLTELHREGVLDDFEQIDKDGQPDFKLTFEGHDYLLECKNVEKEKRPGGPMTVDFQ
                     RTRAPKEAPHLRYYHEDEFELLAACTFNRSGRWEFRFIRTAVLDRRMAPHNDRFLNRV
                     YVDDSASYGKKWSSDLTSILRELSG"
     gene            1106..1429
                     /locus_tag="VFJ72_02365"
     CDS             1106..1429
                     /locus_tag="VFJ72_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET7478340.1"
                     /translation="MTSTVTQPRQENKQRTKELPPYNVVLLDDDDHSYEYVILMLRKL
                     FGHSVNKAYGMAQEVDATGRVVVATTNLELAELKRDQIQSFGPDPLIPRCKGSMSATV
                     EPASS"
     gene            complement(1437..3047)
                     /locus_tag="VFJ72_02370"
     CDS             complement(1437..3047)
                     /locus_tag="VFJ72_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7478341.1"
                     /translation="MTGQFSWWQDGVIYQIYPRSFADASGDGVGDLRGIAGRLDYLEW
                     LGVDAVWLSPFYPSPMADFGYDISDYRGVDPLFGTLEDFDELLEEARRRGIRMVIDLV
                     PNHTSDEHPWFAESRSSRDNPKRDWYVWHDPKPDGSPPNNWESVHGGGSCWKWDEKTR
                     QYYLHTFQVEQPDLNWRNPRVREAMYDVMRFWLDRGVDGFRLDALSFCVKDDRLRDNP
                     PNPGWKPGDPPWTRQIRLHSDDQPEMMDVVREMRAVADEYGGGRVLIGELYLPLERLI
                     AYYGTDLDGVHLPFNFGLVDLAKWEPRAVGRLISAYETALPEGAWPNWVLGNHDQSRV
                     ATRLGEERARLAQMLLLTLRGTPTCYYGDEIGMQDVPVHQDLVQDPQGKLSPGHSRDP
                     ERSPMQWDASPNAGFCPPEADPWLPITGDYKEVNVEAQKEASRSVLSLFRRLVGLRRE
                     HPALISGSYEPLDPGDENVLAYQRSDGDSRALVVLNFGPRTARPRLPKRLAASGAKLL
                     CSTDPERKSLVDADDLELGPYEGVLLST"
     gene            3185..4528
                     /locus_tag="VFJ72_02375"
     CDS             3185..4528
                     /locus_tag="VFJ72_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7478342.1"
                     /translation="MYKATSRSGSIPLSRRAMSRRSFLKLGGAGLAGAALLGATGCGG
                     GGGGSSESGDIVFANGPDQSGATEKLLKKFNEQSKDFKVKFREMPADTGQNFDKMRTQ
                     FQAGSGDIDVFAGDVIWPAQFAANGWLMDVSDRFPESARKDFLEGPIQSLTYDGKVYG
                     VPWFTDAGMLYYRKDLLDKAGLKPPQTWDELIQQADQVSKSENVKYGFVYQGSNYEGG
                     VCNGLEYIWTHGGDVLDPNDASKVIIDSPESAAGLATERRTISDGAAPQSVTNFTETE
                     SQTTFLNGDAVFCRNWPYMYALAANPDESKIKQEQVGVTALPVKEGQPTSSALGGWNM
                     LINANSQKQDQAWEFVQWMTDPAQQKFRALQGSFLPVATSLYNDKEIKEKVPVVSLGQ
                     EALKSSRPRPVSPAYSDMSLKMGEQFAAVLKGDVSPDKAVKTLQEELQSIADQVS"
     gene            complement(4772..>5284)
                     /locus_tag="VFJ72_02380"
     CDS             complement(4772..>5284)
                     /locus_tag="VFJ72_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7478343.1"
                     /translation="TYSYGHRNVQGLAWDESGQLYASEFGQDRFDEVNKIEAGGNYGW
                     PEVEGAGGEPRFIDPIQTFATDEASPSGATILKDSAIPQWDGDFFMAALRGARLWRLE
                     LGPSGDVTGTEALLTGEYGRLRNAVQAPDGSLWVFTNNRDGRGTPTEGDDRILRLAPA
                     RGSARGSARG"
BASE COUNT         1043 a         1648 c         1602 g          991 t
ORIGIN      
        1 tcccttcgca ggctctaaca tgacgggtta cgcagccgag aaagccggga ggagatggat
       61 atcaatcgag ttaaacgaag agtatctgga gacgtcacgc ttccgcttcg ttcagcctgt
      121 tttgttcgaa gagtccgccg cccaaaacta cggcaatcct gaataaactc taccggtcca
      181 ttttctcgac cagacgaatt atctcgctaa gaggtagttc caacgcttgt gctatacgct
      241 gcgccgagtc cagggtgggt tttctgactc cgcgctcaac catgctgata aaggtacgat
      301 caagctcggc catcgcggcg agagcttctt gggagagtcc acgttccaag cgcaacctac
      361 gaacctcgct cccaaatcgg gaagaaaacg aattagccac tcagctcacg aagaatgcta
      421 gtcagatccg atgaccattt cttaccgtaa ctcgccgaat catctacgta aacacgattc
      481 aggaacctat cattatgcgg ggccatcctg cggtcgagaa cagcagttcg aataaaacgg
      541 aactcccacc taccacttcg gttgaaagtg caggccgcaa gtaactcaaa ttcatcctcg
      601 tgatagtaac ggagatgcgg agcttccttg ggagcgcggg ttctctgaaa atccacagtc
      661 atgggaccgc caggtcgctt ctccttctcc acgttcttgc actcgagcaa gtagtcgtgg
      721 ccttcgaagg ttagcttgaa gtccggctga ccgtctttgt caatctgctc gaagtcgtcg
      781 agaacaccct cgcgatgtag ttccgtaaga tactttcgca gatgctcctg cgccacggct
      841 ccctttactg ctatgtgcaa acggttgttg gagagaacca tgtccaatat ttcgacagag
      901 tccatctgaa actgaacttc aagctgatgc agctttctct tttcttccac gcaaccgcac
      961 ctctgctcag ataaatacaa tgagcgccag cgtacctgcg tgacgtagaa gtcactacgg
     1021 actatagtct acaaagtgcc gggagaacta aaaagctgct gaaagtctgg ggagagtcac
     1081 cctatcatgt taagctggca gcgcaatgac gagtaccgtt acccagccca ggcaagagaa
     1141 caagcagcgg acgaaggagc ttccgccgta taacgtggtc ctgctcgacg atgacgatca
     1201 cagctacgag tacgtgatcc tgatgttgag gaagctcttc gggcattcgg tgaacaaggc
     1261 gtatggcatg gcgcaggagg tagacgcgac cggcagggtc gtcgtggcga ccacgaacct
     1321 ggagttggcg gagttgaagc gcgaccagat ccagagcttc ggaccagacc cgcttatccc
     1381 gcgctgcaag ggctcgatgt ccgcaacggt cgagcccgct tcttcctgac actaccttaa
     1441 gtgctcagta gcactccctc gtatggcccc agttcgaggt cgtccgcgtc cacgagactc
     1501 ttccgttccg ggtccgtgga gcacagcagc ttcgccccgg acgcggccaa ccgtttcggg
     1561 agccgcggcc gcgcggttcg cgggccgaag ttgaggacca cgagagcgcg gctgtcgccg
     1621 tcgctcctct ggtaggcgag gacgttctcg tctcctgggt ccaggggctc gtaggagccg
     1681 gagatgaggg cgggatgctc gcgccggagc ccgacgaggc gccggaagag ggagagtacg
     1741 gagcgagagg cttccttctg ggcctcgacg ttgacttcct tgtagtctcc ggtgatgggc
     1801 agccacgggt ctgcctcggg cgggcagaag ccggcgttgg gtgaagcgtc ccattgcatg
     1861 ggagatcgtt ccgggtcccg gctatgtccg gggctcagct tgccctgcgg gtcctggacg
     1921 agatcctggt ggaccggcac gtcttgcatg ccgatctcat caccgtagta gcacgtcggg
     1981 gttccgcgca gggttagcag gagcatctgg gcgagccggg cccgctcctc gccgagccgg
     2041 gtggctaccc gggactggtc gtggttgccg agcacccagt tcggccacgc gccctccggc
     2101 agcgcggtct cgtaggcgga gatcaggcgt cccaccgccc gcggctccca cttcgccaga
     2161 tccacgagcc cgaagttgaa gggcaaatgc acaccgtcga gatcggtccc atagtaggct
     2221 atcaggcgct ccaggggcag gtagagctcg ccgatcaaga cccggccgcc cccgtactcg
     2281 tccgccacgg cgcgcatctc gcgtaccacg tccatcatct cgggttgatc gtccgagtgg
     2341 agccggatct ggcgcgtcca gggcggatcc cccggtttcc atccggggtt cggcgggttg
     2401 tcccgcaagc ggtcgtcctt tacgcagaaa gagagggcgt cgagcctgaa accgtccacg
     2461 cccctgtcga gccagaaacg catgacatcg tacatcgcct cccttacccg cgggttacgc
     2521 cagttaaggt cgggctgctc tacctggaat gtgtgcaggt agtactgccg cgtcttctca
     2581 tcccatttcc agcagctacc gccgccgtgg acgctctccc agttgttcgg cggcgagccg
     2641 tccggcttcg ggtcgtgcca gacgtaccag tccctcttgg ggttatcgcg ggaggagcgg
     2701 gactccgcga accaggggtg ttcgtccgag gtgtggttgg ggacgaggtc gataaccatt
     2761 ctgatgccgc gccggcgcgc ctcttccagc aactcatcga agtcttcgag cgtcccaaag
     2821 agagggtcca cgccgcgata gtcggagatg tcatagccga agtccgccat cggggaggga
     2881 tagaaaggcg agagccagac ggcgtccacc ccgagccatt ccaggtagtc gagccgcccc
     2941 gcgatgccgc gcaggtcccc gacgccgtcg ccgctcgcgt ccgcgaaaga gcgcggatag
     3001 atctggtaga tcactccgtc ctgccaccat gagaattgcc ctgtcaagtc gtcctcctta
     3061 atgccggatc cttattatga ggggtccgcc ggttcgcctc gttcgagggc gatttgcggc
     3121 ggcttcgccg ttagattact ctgtttgcaa ataattgggg actggacaga gaaaggcagg
     3181 gtctatgtac aaggcaacgt ccagaagcgg cagcatcccc ctctcgcggc gagcgatgag
     3241 ccggcgcagc ttcttgaagc tcggcggcgc cggtctcgcg ggggcggccc ttttgggcgc
     3301 gacgggctgc ggcgggggcg gcggaggctc cagtgagtcc ggcgacatcg tcttcgccaa
     3361 cggcccggac cagagcgggg ccacggagaa actgctcaag aagttcaacg agcagagcaa
     3421 agacttcaag gtcaagttcc gggagatgcc ggctgatacc ggccagaact tcgacaaaat
     3481 gcgcacgcag ttccaggcgg gcagcggcga catagacgtg ttcgcgggcg atgtgatctg
     3541 gccggcccag ttcgccgcga acggctggct catggacgtg agcgaccgct tcccggaatc
     3601 cgcgaggaag gacttcctcg aaggtccgat acagtccctt acctacgatg gcaaggtcta
     3661 cggcgtgccg tggttcacag acgcggggat gctctactac cgcaaggacc ttctggacaa
     3721 ggccggtctg aagccgcccc agacctggga cgagctaata cagcaggcag atcaggtctc
     3781 caagtccgag aacgtcaagt acggcttcgt ctaccagggg tccaactacg agggcggggt
     3841 ctgcaacggc ctagagtaca tctggacgca cggcggcgac gtcctggacc cgaacgacgc
     3901 cagcaaggtc attatagata gccccgagtc cgctgcgggt ctcgccactg aacgccgcac
     3961 catctcggac ggcgcggccc cccagtccgt caccaacttc acggagaccg aatcgcagac
     4021 caccttcctc aacggcgacg cggtcttctg ccgcaactgg ccttacatgt acgcgctggc
     4081 cgccaacccg gacgagtcga agattaagca ggagcaggtt ggcgtcacag cgctgcccgt
     4141 gaaggaaggc cagccaacca gcagcgccct cggcggctgg aacatgctca taaacgccaa
     4201 ctctcagaag caggaccagg cgtgggagtt cgtgcagtgg atgaccgacc cggcgcagca
     4261 gaagttccgg gctctccagg gctcgttcct tccggtggct acctccctgt acaacgacaa
     4321 ggagatcaag gagaaggtgc cggtcgtctc cctgggccag gaagccctca agagctccag
     4381 acctcgcccc gtgtcgccgg cctactctga tatgtccctc aagatgggtg agcagttcgc
     4441 cgccgtgctc aagggtgacg tctccccgga taaagccgta aagacacttc aagaagagct
     4501 acagagcatt gcggatcagg tctcgtaact gacgctgcct ccaccgggac ggcgcgcctc
     4561 cgcctcgcga cacacgcgcc gtcccgcaag agtcctcggt attcgaggag tcacgcggat
     4621 agtgagatat acgaccccgg actcccacca cgcccattcg acgccttccg ccctatgttc
     4681 cgtcgctctt ctcacgtagg gttcgaccgg acaccgggat gctcccgcca tgtccgccaa
     4741 gagccggaag ctctacacga accgcagcat tctagccacg tgcggaacca cgtgctgaac
     4801 cgcgtgcggg agcgagccgc aggatacggt cgtcaccctc ggtgggcgtg ccgcgtccgt
     4861 cgcggttgtt ggtaaagacc cacagagaac cgtcgggggc ctgaacggcg ttcctgagcc
     4921 gcccgtattc gcctgtcagc agggcttccg tgccggtcac gtcgccgctc gggcccagct
     4981 ccagacgcca gagccgcgct ccccggaggg ccgccatgaa gaagtcgccg tcccactgcg
     5041 ggatggcgct gtccttcaga attgtggctc cactcgggga ggcttcgtca gtggcgaagg
     5101 tctggatcgg gtctatgaac ctcggctctc cacccgcgcc ctcgacctcg ggccagccgt
     5161 agttgccgcc ggcctctatc ttgttgacct cgtcgaagcg gtcctggccg aactcacttg
     5221 cgtacaattg tccgctttcg tcccaggcga ggccctgcac gttgcggtgt ccgtaggagt
     5281 acgt
//
LOCUS       DASSMB010000031         2135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1528091, whole genome shotgun sequence.
ACCESSION   DASSMB010000031 DASSMB010000000
VERSION     DASSMB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1528091"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="VFJ72_02385"
     CDS             <1..1162
                     /locus_tag="VFJ72_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7478344.1"
                     /translation="KQLFTFRVPVGVAAIITAGNFPVAVPSWYLIPALTCGNTVVWKP
                     AEYSPAIGDALAQLFNRGGLPDGVLNVVQAEGPAAFEGLERALDEGLVDKVGFTGSSE
                     VGKKIGELCGRHMQSPCLELGGKNPLVVMPDADLDLAVEGALFSGFGTAGQRCTSLGT
                     VIAHESIYDEFVSKFARAVDEAKIGDPTQDVLYGPMLSQRFHDRFIGWLELIEDHHSL
                     HGSTGMGTITKDNPREGFVGDPESGIYCHPTIVDGVTMEDEIYRTETFGPIVGVARFS
                     DFDEAVALSNNHGYGLSAAIYTRDATNALRFRERNKAGMLSVNNSTSGAEAHLPFGGN
                     GKSGNGSRLSGIWVIDQFTRWQSMNWDYAGKLQKAQMDIADLPADLDYRLPE"
     gene            1169..>2135
                     /gene="solA"
                     /locus_tag="VFJ72_02390"
     CDS             1169..>2135
                     /gene="solA"
                     /locus_tag="VFJ72_02390"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET7478345.1"
                     /translation="MYDVIVAGLGGMGSAAAYHLAGRGKKVLGLEKHTPAHDRGSSHG
                     QSRIIRQAYFEGAEYVPLLLRAYELWEQLEKQTSEELMTLTGGLMIGAPESRLVAGST
                     HSAEEHGLPYEILDSSELKRRYPPFEPAPNTVALYEKQAGFVRPEASVRAHLDRAASL
                     GADLHFEEPMVSWEATTSGVRVRTERDVYEAERLVIAPGAWAPGLLADLGLPLEVTRQ
                     ILFWFDPIGGIEPFLPDRFPIFIWEPEDGNAFYAFPAHDGPDWGVKVAFFRADGTPAD
                     PETIDREVHGEEVEFIRRYLARHVPALNGDLLHTKTCMYTNTPDEH"
BASE COUNT          379 a          709 c          700 g          347 t
ORIGIN      
        1 caagcaactc ttcactttcc gggtgccggt aggggtcgcg gccattatca cggccggcaa
       61 cttcccggtc gcggtgcctt cctggtacct gatcccggcc ctcacctgcg gcaacaccgt
      121 ggtctggaag cctgccgagt actcccccgc catcggcgac gccctcgcgc aactctttaa
      181 ccgtggcggc ctcccggacg gcgtcctgaa cgtggtgcag gccgaaggac cggcggcttt
      241 cgaggggctg gaacgggcgc tggatgaagg actcgtggat aaggtcggct tcaccggctc
      301 ctcggaggtc gggaagaaga taggcgagct gtgcggccgg cacatgcagt cgccgtgcct
      361 ggagcttggc ggcaagaacc cgctcgtcgt catgcccgac gcggacctcg atctcgccgt
      421 ggagggcgcg ctcttctccg gcttcgggac agccggacag cgctgcactt cgctcggtac
      481 tgtcatcgcg cacgagtcca tctacgacga gttcgtctcg aagttcgccc gcgccgtcga
      541 tgaggcgaag atcggggacc cgacccagga cgttctatac ggcccgatgc tcagccagcg
      601 cttccacgac cgctttatcg ggtggctgga actgatagag gaccaccact ccctccacgg
      661 ttccaccggg atggggacca tcacgaagga caacccgcgg gagggcttcg tcggcgaccc
      721 ggagagcggt atctactgcc accccactat cgttgacggc gtaacgatgg aggatgagat
      781 ctaccggacc gagaccttcg gtccaattgt cggcgtcgcg aggttctcgg acttcgatga
      841 ggccgtcgcc ctctccaaca accacggcta cggtctctcg gcggcgatct acacccgcga
      901 cgccactaac gccctgcgct tccgggagcg gaataaggcc gggatgctct cggtcaacaa
      961 ctccaccagc ggcgccgagg ctcacctgcc cttcggcggc aacggcaaat ccggcaacgg
     1021 aagccggctc tccggtatct gggtcataga ccagttcact cgctggcagt ccatgaactg
     1081 ggactacgcc ggcaagctcc agaaagccca gatggacata gccgacctgc ccgccgacct
     1141 cgactaccgg cttccggagt agaggagcat gtacgacgtc atagtggccg ggctcggcgg
     1201 gatggggagc gcggcggcct accatctcgc cggccgcggc aaaaaggttc tcgggctgga
     1261 gaagcacacc ccggcgcacg ataggggctc cagccacggc cagtcgcgca tcatccggca
     1321 ggcgtacttc gagggcgcgg agtacgtgcc gctactcctg cgggcctacg agctgtggga
     1381 gcaactggag aagcagacca gcgaggaact gatgacgctg acgggcggac tcatgatcgg
     1441 cgctcccgag agccggctag tcgccggcag cacgcacagc gccgaggagc acggtctgcc
     1501 gtatgagatc ctggactcct ccgagctgaa acgccgctac ccgcccttcg agcccgcgcc
     1561 caacacggtc gctctgtacg agaagcaggc gggtttcgtg cgcccggagg cgagcgtcag
     1621 ggcacacctt gaccgggccg cctctctcgg cgcggacctt cacttcgagg agccgatggt
     1681 ctcgtgggag gccacgacat caggggtgcg ggtgcggacg gagagagacg tatacgaggc
     1741 ggagcggctg gtgatcgccc caggggcgtg ggcgccgggg ttgctggcgg acctggggct
     1801 accgctggag gttacgcgcc agatcctgtt ctggttcgac ccgataggcg gcatcgagcc
     1861 tttcctgccg gaccgcttcc ccatctttat ctgggagccg gaggacggca acgcgttcta
     1921 cgccttcccg gcgcacgacg gtcctgactg gggcgtcaag gtcgcgttct tccgggccga
     1981 cgggacgccg gccgacccgg agacaataga ccgtgaggtg cacggcgagg aggtggagtt
     2041 catccggcgc tacctcgctc gccacgtccc ggctctgaac ggagatctcc ttcacaccaa
     2101 gacctgcatg tacactaaca cccccgacga gcact
//
LOCUS       DASSMB010000032         4106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1653766, whole genome shotgun sequence.
ACCESSION   DASSMB010000032 DASSMB010000000
VERSION     DASSMB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653766"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VFJ72_02395"
     CDS             <1..184
                     /locus_tag="VFJ72_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478346.1"
                     /translation="ELGRFRPHDVRSSARAFIGMLLPQAGGKMFFPAIREDGLTDEEH
                     IENAIKLFLEGLKPES"
     gene            194..1042
                     /locus_tag="VFJ72_02400"
     CDS             194..1042
                     /locus_tag="VFJ72_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7478347.1"
                     /translation="MDSIEVTGLTKRYGKIEALQGVDLTVPEGSVFGLVGPNGAGKTT
                     LIKALVGALRPTSGDARVLGLDPLRDRAELRRQIGYMPQSPALYEDLSARDNVRFFGA
                     AHGVPEIHKKTDEVLAFTELAGRAKDPLYTFSGGMKRRVSLACALVHRPEVLFLDEPT
                     AAVDPQLRSRFWKTFRKLAGEGATLFISTHLMEEAMLCDRVAVLRKGRVIASDTPRNI
                     LERGKTRLVLSRSGDEEEKTIGGHPEDLAAALHPYGLAPEVTAVEVEADTLETVVLSL
                     IEEEAR"
     gene            1039..1776
                     /locus_tag="VFJ72_02405"
     CDS             1039..1776
                     /locus_tag="VFJ72_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7478348.1"
                     /translation="MRHTFTVARRVIRQLLHNRRFLALSIVAPLLIVYFLKLFFDTMP
                     EGFDVARYIVPVAAFIVHFLAFILCAISLVQERTAGTMERMFINGFRRAEIIGGYMLG
                     FLGLATFQAVAALTEAIWLFELDYDTQTLVTLFGVVWLLAIASVMLGIFVSTFARHEG
                     QVFPFIPLIIFPSVFLSGLLMDIEDLPTWAEWLGHAFPLFYANDVIQEVIKPDGSLGD
                     VWGNLVILAGYGVVLLLLASRTLPEVE"
     gene            1794..3038
                     /locus_tag="VFJ72_02410"
     CDS             1794..3038
                     /locus_tag="VFJ72_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7478349.1"
                     /translation="MAEMTTQARSVLRTTCCVVGGGPAGAMLALLLARGGVDVTLLEA
                     HPDFDREFRGDTLHPSVMEIMAELDLADRLLELRHSEVHSLTFQTPDGPFTPVNFRRL
                     KSRFPYITMMPQKSFLEFITGEAKRYPNFRLVMRARVRELVEENGVVRGVRYEGEDGV
                     HEVRAILTVGADGRGSRVRKLADFEPVRTSPPMDVLWFKLPRKEGDPEDAMGRFGRGE
                     IAILLNRFDYWQAGYVIPKGTFSRLRREGIEALRRNFAGLVPDFADRVERITDWQHVS
                     LLSVESSRCPRWYRPGLLVIGDAAHVMSPVGGVGINYAIQDAVVAANVLAEPLKESQR
                     RLTDLHTRYLASVQRRRELPTRLIQRLQSQVQQRVLAPALDSDRPFKLPLLARLVFDT
                     PVLRNIPTRVVGLGFWPVRVKK"
     gene            3049..>4106
                     /locus_tag="VFJ72_02415"
     CDS             3049..>4106
                     /locus_tag="VFJ72_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET7478350.1"
                     /translation="MSTVHDIGVKTIVPFEDLDRTSLPIAGGKAANLGEMIRAGLPVP
                     AGFCATTAAYERVSRDAGLDAILTALSGTPPDDAARLSQLAAEARDRLSAAPIPEDLA
                     RSITAAYLELGDGTSVPVAVRSSATAEDLPFASFAGQQDTCLNVVGEKAVLDAVRRCW
                     ASLWTDRAVSYRATNGIDPHGVRLAVAVQRMVAAEVAGVLFTANPLTGKRRQAVIDAS
                     PGLGESVVSGAVNPDHFVVNTTTGEIVERRLGDKQVAIRAAEGEGTQRVELAGQEDLA
                     CLSDEQVRALAALGARVEAHYGAPQDTEWAIDAGGGIWLVQARPITTLFPLPANAPAT
                     DDAPRVYFSGSVAQGVYQP"
BASE COUNT          682 a         1377 c         1365 g          682 t
ORIGIN      
        1 cgagctaggc aggttccgtc cgcacgacgt ccggtccagc gcccgcgcgt ttatagggat
       61 gctcctcccg caggcgggag gcaagatgtt tttcccggct atccgggaag acggtctgac
      121 cgacgaagag catatagaga acgcgatcaa gttattcctc gaaggactga agcccgaaag
      181 ttaggagcag gttgtggact ccatagaggt tacggggctg accaagcgct acgggaagat
      241 cgaagccctg caaggcgttg acctcacggt tccagaggga tcggtcttcg ggctggtagg
      301 gcccaacgga gccgggaaga cgaccctcat caaggccctc gtcggggcgc tgcgccccac
      361 gagcggcgac gcgcgcgttc tcggactcga ccctctgaga gaccgggccg aactccggcg
      421 gcagatcggc tacatgcccc agtcccccgc cctatacgag gacctctcgg cccgggacaa
      481 cgtccggttc ttcggcgccg ctcacggggt gccggagatc cacaagaaga ccgacgaggt
      541 gctcgccttc accgagctgg ccgggcgcgc gaaagatcct ctgtacacgt tctccggcgg
      601 gatgaagcgc cgcgtctccc tcgcctgcgc tctggtccac cggccggagg tgctgttcct
      661 cgacgagcca acggctgcgg tggacccgca gctcaggagc cggttctgga agacgttccg
      721 caagctcgcc ggggaaggcg cgacgctgtt tatcagcact cacctgatgg aagaggcgat
      781 gctgtgcgac agggtcgcgg tcctgcgcaa aggtcgcgtc atagcctccg acacgccgcg
      841 caacatactg gagcgcggca agacccgcct ggtgttgagc cgtagcggcg acgaggaaga
      901 gaagaccatc ggcgggcatc ccgaagacct ggcggccgcg ctgcatccgt atgggctggc
      961 gccggaggtc acggcggtgg aagtggaggc cgacacgctg gagacggtcg tgctgtcgct
     1021 aatagaggag gaggcgcgat gagacacacg ttcacggtcg cccggcgggt cataaggcaa
     1081 ctcctgcaca accgcaggtt cctggcgctc tccatcgtgg ctccgctcct gatcgtctac
     1141 ttcctcaagc tcttcttcga caccatgccc gaaggcttcg atgtcgcgcg ctacatagtc
     1201 ccggtggcgg cgttcatcgt gcacttcctg gccttcatcc tgtgcgccat ctcgctcgtg
     1261 caggagagga cggcggggac gatggagcgc atgttcataa acggtttccg gcgggccgag
     1321 atcatcggcg gatacatgct cgggttcctg gggcttgcga ccttccaggc cgtcgcggcg
     1381 ctcaccgagg cgatctggct cttcgagctg gactacgata cgcagacgct cgtcaccctg
     1441 ttcggcgtcg tgtggctgct cgcgatagcc tccgtcatgc tcggcatctt cgtctcgacc
     1501 ttcgctcggc acgagggaca ggtcttcccc ttcataccgc tcatcatctt cccttcggtc
     1561 ttcctctcgg gactgctcat ggacatcgaa gacctgccta cctgggcgga atggctggga
     1621 cacgccttcc cgctgttcta cgcgaacgac gtgatccagg aggtaataaa accggacggc
     1681 tctctcggcg acgtgtgggg caacctcgtc atcctggcgg gctacggcgt ggtccttctc
     1741 ttgctcgcgt cccgcacgct cccggaggtc gagtaacgcg aaaggagtcc ctcatggccg
     1801 agatgacaac gcaggctcga agcgtgctgc ggactacgtg ctgcgtcgtg ggcggcggcc
     1861 cggcgggggc catgctggcg ctgctgctgg cgcgtggggg cgtggacgta accctgctcg
     1921 aagcgcaccc ggacttcgac cgcgagttcc ggggcgacac tctccaccct tcggtcatgg
     1981 agatcatggc ggagttggac ctcgccgacc gcctgctaga actccgccac agcgaggtgc
     2041 atagcctgac cttccagacg ccggacgggc ccttcacacc cgtgaacttc cgccgcctca
     2101 agagccgctt cccctacata acgatgatgc cccagaagag cttcctggag ttcataaccg
     2161 gagaagcgaa gcgctacccc aacttccggc tcgtgatgcg ggcgcgggtg cgagagctgg
     2221 tcgaggagaa cggcgtggtt cgcggcgtcc ggtacgaggg cgaggacggc gttcatgagg
     2281 tacgcgcgat ccttaccgtc ggcgcggacg gtcgcgggag ccgggtacgg aagctggccg
     2341 atttcgagcc ggtcaggacc tcgccgccga tggacgtcct ctggttcaag ctgccccgga
     2401 aggaaggcga tccggaggac gcgatggggc gtttcgggcg cggggagatc gccatcctcc
     2461 tcaaccgctt cgactactgg caggctggat acgtcatccc caaaggcacc ttctcccggc
     2521 tgcggcgcga ggggatcgag gcgctcagac gtaacttcgc cggcctggtc ccggacttcg
     2581 cggatcgcgt cgagcgcata acggactggc agcacgtctc gctgctctct gtggagtcga
     2641 gccgctgtcc gaggtggtac aggccggggc tgctcgtcat cggggacgcg gcgcacgtca
     2701 tgagcccggt cggaggggtc ggcatcaact acgccattca ggacgcggtc gtcgccgcga
     2761 acgtgctggc agagccactc aaagagagcc agcgccgcct gacggacctg cacacccgct
     2821 acctggcgtc cgtgcagcgc cgccgcgagc tgccgacgcg gctgatacag cgtctccagt
     2881 cccaggtcca gcagcgcgtc ctcgcgcccg ccctcgactc ggaccgtccg ttcaagctgc
     2941 cgctgctggc gcggctcgtg ttcgacaccc ccgtcctgcg caacataccc acccgcgtcg
     3001 tcggactcgg cttctggccg gtccgggtga agaagtagag gagtatccat gagcacggtt
     3061 cacgacatcg gcgtaaagac aatagtcccc ttcgaggacc tggaccggac ctcgctcccc
     3121 atcgctggcg gcaaggcggc caacctggga gagatgatcc gggccggcct cccggtcccc
     3181 gccgggttct gcgcgaccac ggcagcctac gagcgcgtct ccagagacgc gggcctggat
     3241 gccatcctta ccgccctatc cgggacgcct cctgacgacg cggcgcgtct gtcgcagctc
     3301 gccgcggagg cgcgagaccg cctgtcggcg gcgcccatcc cggaagatct tgcccggtca
     3361 attaccgcgg cgtatctgga actaggggat ggaacgtctg tcccggtggc tgtccgctcc
     3421 tcggccacgg cggaggacct gcccttcgcg agcttcgccg gccagcagga cacgtgcctg
     3481 aatgtcgtgg gcgaaaaagc cgtgctggac gcggttcggc ggtgctgggc ctcgctctgg
     3541 acggaccgcg ccgtaagcta ccgcgccacg aacgggatag atccccacgg cgtgcgtctc
     3601 gcggtcgccg tgcagcggat ggtcgccgcc gaggtagccg gcgtcctctt caccgcgaac
     3661 ccgctgacgg ggaagcgccg ccaggccgtc atagacgcca gccccggcct gggcgagtcg
     3721 gtcgtctccg gcgcggtcaa ccccgaccac ttcgtcgtga acacaacgac cggcgagatc
     3781 gtcgagcgcc gcctcggcga caagcaggtc gccatccgag ccgccgaggg tgagggcacg
     3841 cagcgcgtcg agctagccgg acaagaagac ctggcctgcc tctcggatga gcaggttcga
     3901 gcgctggcgg cgctcggagc gcgggtcgag gcgcactacg gagcgccgca ggacacggag
     3961 tgggccatcg acgccggcgg tggaatttgg ctggtccagg cgcgcccgat cacgaccctc
     4021 ttccctctcc cggccaatgc cccggcgact gacgacgccc cgcgcgtcta cttctcgggc
     4081 agcgtcgccc agggcgtcta ccagcc
//
LOCUS       DASSMB010000033         9824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_640289, whole genome shotgun sequence.
ACCESSION   DASSMB010000033 DASSMB010000000
VERSION     DASSMB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9824
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640289"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VFJ72_02420"
     CDS             <1..641
                     /locus_tag="VFJ72_02420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7478351.1"
                     /translation="RTSAMRQKLLSEERFMISHVGRLTHEKRPQDLISFAENLAQRRQ
                     DWRLFIAGTGSLRRTLERQTVRLRLADRVKFLGYISEEEKEDLLMASDLFLMPSPTEL
                     QSIATLEAMARSCAVVAAGFETSAVGEMVREAGCGLCYRPERMDEAAEDTSRLLDEPD
                     RLRVLQRNAEDAARRHDVHLSGKRLEEVYASLLRVRDGDPEPVAGPLERMEA"
     gene            638..2512
                     /locus_tag="VFJ72_02425"
     CDS             638..2512
                     /locus_tag="VFJ72_02425"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7478352.1"
                     /translation="MNDGMVAVTGAGGSLGSALTRRLLRGGHAVRALVRREEDAHRLR
                     EMGATPIVGDVRDGRALESLVRGCEAVFHLAAWMGKPFDEELAHAVNVGGAEGVVRAA
                     AAGGARRVVLASSIAVYGPVLRGVVTEDAPLWSVGDMYGDTKIAAERAARREAERLGI
                     ELVVLRPTMIFGPESPSWTEEPFAAISKGLPAIIGSGEDLVDPVYVEDVARAFELAAS
                     IPEAAGETFNIGAGPRTWNEFFGAYATMAGKPLRRIPASVTRRAAQAGERAERLLGRR
                     PRLPSEMVGVMTSRAVFSTEKAKRVLGFEPAYSFEEAMAETEAWLRSAGRLRRPSVAL
                     VTGAAGGLGWAVARRLREEGLTVWAADIQREALGGLAVEGIRTVALDVGSEESVRAVV
                     GEIEAESGPVDLLVNVAGVGRPGPLESQPFGEVESHFDVNAYGPLRLARAVAPGMRRR
                     GWGRILNVTSTNGFIVTPFMGAYSASKHALEAISDALRMELKPWGVEVGVIQPGAMKT
                     AFAGRAQAALKASISGSNDGWSPYLKSFLDSSLWGTATATSPERVARVVARAALANRV
                     PARSLATLDAIPARVMSMMPTAVRDAFLVRAAGLSQAPERDEASRNRAASPPPARSGR
                     "
     gene            complement(2403..3428)
                     /locus_tag="VFJ72_02430"
     CDS             complement(2403..3428)
                     /locus_tag="VFJ72_02430"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7478353.1"
                     /translation="MKRALAMLFLSLALGFGGLYLAVGGALFEAETYRIRDLSAFFLI
                     VGVIALAAEWMTPGLKVSLLCRGQGIQMPYRPALLAHLVGVLGAALTPSNTGGVPATV
                     AALGRLGVPVGKGIGVIVQVFILDLIFFSWSAPLGLGYLIYTRAISLPIRAEFLTVVA
                     VSLAVAGSIILSRHPRLVVRLLLALARWPLLGRFQTRLTNTARDYQRSARAYMRMSAR
                     TWVSLHAITAVGWIAGFVILWSLLHLYGVSAGLPTVLSLLASIILVSHFIPTPGGAGF
                     IEAAVALSVGAGVGSVAAAVLLWRLLSFYLIFLIGPVAGWLLYFSKPRLSPGPAKVPP
                     PGPGKRP"
     gene            complement(3432..4595)
                     /locus_tag="VFJ72_02435"
     CDS             complement(3432..4595)
                     /locus_tag="VFJ72_02435"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7478354.1"
                     /translation="MARVSAGRPGILFATVAVGGGHVATARAISEAIEYHYPDQFSPR
                     VSDYMEDLGVLNADRRHKAFWRLALRYPFIARAGQRLIDSFPRLTVAGQRRLVGEFAR
                     TAAAELEKERPLLVVSNHGLLTTGLALARDRYGLQVPVLTYATEPYNISAYWADPRAE
                     HVVVPAAEVRRDLIHMGVPPDKISVVSYPVRQPFLNAPSKAEARARLGLEGRFTCLVT
                     LGGEGVGGHPHHIVPALLHSEAAPQVVVVTGRNKKLRHALEELRAPGLRVEGFVEEMA
                     LHVAASDVVIGKAGPASVYEALAVGRPVLVTGYAGLNELGVAKFVEREGFGHYVRTSE
                     ALSKAVARYAASPALLEEVALRSRDLDLAGDTERLARYVVRYALNNASDGAAG"
     gene            complement(4750..6600)
                     /locus_tag="VFJ72_02440"
     CDS             complement(4750..6600)
                     /locus_tag="VFJ72_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478355.1"
                     /translation="MRSAVAAMVLSMVFLFPLAQAASGEPDHAPVVLVLADSGLTWKS
                     VEYPGLAPVFDNAAVASLSTSQGQAPEDPRMNYVLLGAGARADTSVLPENLPQERGKI
                     PGSFTGPSATVHPGALGDALAHSGARAASVGGRAALVVMDSKGRVPITYGSKEPVKHL
                     KDALLHGADLVAVEASDPREAGEAADAARQAGAVVAIAAPNAPTGSANLTPFGLAAGD
                     QGGVLYSPGTHTAGLMSSEDVAPTLLEHLGVQAPPEMSGRAAEARPGSMTQVSRLQDR
                     LAFVDEERSVVWTLLSGMSVAAFVLAGILRGWAGIRLAVLFLTALPLGTLAAAAVPVT
                     NALLVAILASLFAAAFVALSRRVSRNPLGELAVVALATAACVISDAALGGWLMRFSIL
                     GHDPAYGTRFYGIGNEYSAVAAGALPLGAALLIMRKPRLLAAVAVAGVLAVLAIGLPT
                     MGADVGGSLALGFGMGATVGLLRGGRMAGVALWAGGGLFLAAALFVSSGFLFPDASHG
                     ARAAGGEAGLYEIAVRKLILSLRHLLNPLWTALLAAGGALIFTALRRLRDTADGKAIA
                     AGMLGATAAAAASGALNDSGILATLLALAYPASAATMVLLDKDRPRRGVA"
     gene            6752..7333
                     /locus_tag="VFJ72_02445"
     CDS             6752..7333
                     /locus_tag="VFJ72_02445"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HET7478356.1"
                     /translation="MFLFREPPDVAVRRFVAGQRGLPFSYSEVGATRGGATAGYVADR
                     YRVRLGEGEETFERAVAALRSWSMFDLGWACLIPDDAPIERGVTVAVLARHLGLWSLN
                     AARIVYVVEDSGSVERRGFAYGTLPGHAARGEERFCVEWDRASDSVHYDVLAFSRPGH
                     PLAWLGYPFVRRLQRRFARDSSAAMLAAARGGP"
     gene            7380..7952
                     /locus_tag="VFJ72_02450"
     CDS             7380..7952
                     /locus_tag="VFJ72_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET7478357.1"
                     /translation="MNATTHAIFGVGALAGAALLTGMEPPLYAYPMAVVAAWLPDVDN
                     PRSTLGNGLSRHKNPLLNAVTRPISWALRTSSFFLIRVVGHRTLTHSLLGLALYALVA
                     WLLLGGYPNLFLALLVGAASHIVADALNTWGVPLLWPLKIQFRLLPGGVRSGGAAEFV
                     VALVVLGLTAYALFEVHPALRGFLGLLQPL"
     gene            complement(7917..8513)
                     /gene="yqeK"
                     /locus_tag="VFJ72_02455"
     CDS             complement(7917..8513)
                     /gene="yqeK"
                     /locus_tag="VFJ72_02455"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET7478358.1"
                     /translation="MEDREPNYVSSETADAFARDRLSEKRYAHTIRVTETAERLARTH
                     ALNEEWTRLAALLHDAAREMSAGEYLRLARDWDLPVGDFERENPKLLHGPVAAELARR
                     KLGVEDEEVLEAIRVHTTGVPGMSYLALAVYVADKIEPARDYPSVGHLRDLAAKDLRA
                     AAREALRRAISYNEQRGRDVHPESRKTLEWLEETEETS"
     gene            8537..9805
                     /locus_tag="VFJ72_02460"
     CDS             8537..9805
                     /locus_tag="VFJ72_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET7478359.1"
                     /translation="MDREQKNIESAEIITIGTEMLLGELVDTNSAWISQRLAELGVGI
                     YRHTTIGDNRDRIVTEMKEAASRSDLVITTGGLGPTSDDLTNECLALAAGREMVEYQE
                     ARDHVDTMFARFGRTPTPSNYKQALFPEGTTLIPNPLGTAPGALLEVDGALFATLPGV
                     PGEMKGMFTETLAPLIRARSDGSIVSRTLWFTGIGESALAEEVQDFLDATDPTVAPLA
                     GQGKVRLRITTRAATPEAAEEKIAPVEKEILSRLDEYYFGQDEETLESAVGRLLREHG
                     ATLAVAESCTGGLIAKRLTDTPGSSAYFEEGLVTYSNEAKERHLGVPREMLVQHGAVS
                     EQVAGAMAEGVRKAAGTDYGLSVTGVAGPDGGTEEKFVGLVYVGVSDAQKTEAERLDL
                     SAWARSRDAIRERSANRALDLLRLRIEEKK"
BASE COUNT         1637 a         3239 c         3441 g         1507 t
ORIGIN      
        1 agcggacctc cgccatgcgc cagaagctcc tttcggaaga gcggttcatg atctctcacg
       61 tcggccgcct gacgcacgag aagcgcccgc aagacctgat ctccttcgcc gaaaatctcg
      121 cgcagcggcg gcaagactgg cgcttgttta tagcgggaac cggctccctc cgccggacgt
      181 tggagcggca aacggtaagg ctccggctcg ccgatagggt gaaattcctc ggttacatct
      241 cggaggagga gaaggaggac ttgctcatgg cgagcgacct cttcctgatg ccgtctccga
      301 cggagttgca gagcatcgcg acgctggagg cgatggcccg aagctgcgcg gtggtcgctg
      361 ccggcttcga gaccagcgcc gtaggtgaga tggtccggga ggcgggatgc ggcctctgtt
      421 accgaccgga gcggatggac gaggcggcgg aagacacgag ccggctgctt gatgaaccgg
      481 ataggttgag agttttgcag cgcaacgccg aagatgcggc gcggcggcat gacgtacacc
      541 tgagcggtaa gcgcctggag gaggtctacg cttccctgct ccgcgtccgg gacggagacc
      601 cggagcccgt ggcggggccg cttgagagga tggaggcttg aacgacggta tggtcgccgt
      661 aaccggggcc gggggatctc tgggctccgc cctcacgcgg cgcctgctgc ggggcggcca
      721 cgccgtgcgg gcactcgtcc gccgggaaga ggacgcacac cggctccggg agatgggggc
      781 gacacccatc gtcggcgatg tgcgcgacgg acgggcgctg gaaagccttg tccgtggctg
      841 cgaggctgtc tttcacctgg ccgcctggat ggggaagccg ttcgacgagg agttggcgca
      901 tgccgtcaac gtcgggggag cggagggagt agtgcgggcc gcggcggctg gtggcgcgcg
      961 gcgggtggtg ctggcgagca gcatcgcggt ctacgggccg gtgctccggg gcgtcgtcac
     1021 ggaggacgcg ccgctctgga gcgtgggtga catgtacggg gataccaaga tagcggcgga
     1081 gcgcgcggcg cggcgcgagg cggagcggct ggggatagag ctggtagtgt tgcgtccgac
     1141 catgatcttc ggcccggagt ccccgtcctg gacggaggag ccgttcgccg cgatctcgaa
     1201 gggcctcccg gcgatcatcg gcagcggcga ggacctggta gacccggtct acgtcgagga
     1261 cgtcgcgcgg gccttcgaac tcgcagcctc catccccgaa gccgccggcg agacgttcaa
     1321 tatcggggcg gggccgcgga cctggaacga gttcttcggc gcctacgcca ccatggccgg
     1381 gaagcccctg cggcgaatac ccgcctccgt aacccggcgg gcggcccagg ccggagagcg
     1441 cgccgaacgc ctcctgggac gccgcccccg ccttccctcg gagatggtcg gcgtgatgac
     1501 gagccgggcg gtcttctcga cggaaaaagc gaagagggtg ctcggcttcg agccagccta
     1561 cagcttcgag gaggcgatgg ctgagacgga ggcatggttg aggagcgccg gacggttgcg
     1621 gcgtccctcg gtcgcgctgg tcacgggcgc ggccggtggg ctggggtggg cggttgcgcg
     1681 caggctccgg gaggagggcc tgaccgtgtg ggcggcggac atccagcgcg aagctctcgg
     1741 cgggctggcg gtggagggaa tccggactgt ggcgctggat gttgggtcgg aggagagcgt
     1801 gcgggctgtg gttggtgaga tcgaagcgga gagtggtccg gtggatctac tggtgaacgt
     1861 cgcgggcgtt ggcaggccgg gaccccttga gagccagccg ttcggcgagg tggagtcgca
     1921 cttcgacgtg aacgcttacg gaccgttgcg gctggcgcgg gcggtcgcgc cggggatgcg
     1981 gcggcggggg tggggaagaa ttctcaacgt cacctccacc aatgggttta tcgtgacgcc
     2041 gtttatgggc gcgtactccg cctcgaagca cgcgctcgaa gccatctccg acgctttgcg
     2101 gatggagcta aagccctggg gcgtggaggt cggcgtcatc cagcccggag ccatgaagac
     2161 ggccttcgcc ggacgcgccc aggcggcgct gaaggcgagc atctcgggct cgaacgacgg
     2221 ctggagccct tacttgaagt ccttcctcga cagctcgctc tgggggacgg ctacggccac
     2281 gagcccggag agggtggcga gggtggtggc gcgggcggcg ctcgccaacc gggtcccggc
     2341 gcgtagtctg gcaacgctgg acgcgatccc ggccagggtg atgtctatga tgccgacggc
     2401 ggtcagggac gctttcctgg tccgggcggc gggactttcg caggccccgg agagagacga
     2461 ggcttcgaga aatagagcag ccagcccgcc accggcccga tcaggaagat gagatagaag
     2521 ctcaggagac gccacaggag cactgccgcc gcgacgctcc ccactccggc gccgacgctc
     2581 agagccacgg ccgcttctat aaaaccggcg ccgcccggcg tggggatgaa gtgcgagacc
     2641 aggatgatgc tggcgaggag cgagaggact gtggggaggc ccgcggacac gccgtagagg
     2701 tggagaagac tccagaggat tacgaagccc gcgatccatc ccacggcggt tatggcgtgc
     2761 agcgagaccc aggtccttgc ggacattctc atgtaggcgc gggcgctgcg ctgatagtcg
     2821 cgagcggtgt tcgtcagccg ggtctgaaag cgtccgagta acggccaacg cgccagggcg
     2881 aggagcaggc gcacgaccag tcggggatga cggctgagga tgatcgaacc cgcgaccgcc
     2941 agagagaccg ccaccacggt caggaactcg gcccggatag ggagtgagat cgctctcgta
     3001 tagatcaggt atcccagccc cagcggcgcg gaccaggaga agaagatcag gtccaggata
     3061 aatacctgca cgatcacccc gatccccttc ccaaccggca ctccgagccg cccgagcgcc
     3121 gcgaccgtgg ccggtactcc tccagtgttg ctcggcgtca gggccgcgcc cagcacacca
     3181 acgaggtgcg ccagcagcgc cggacgatac ggcatctgga tgccctgtcc cctgcacagg
     3241 agagagactt tcaggcccgg cgtcatccac tccgcggcca gcgcgatcac gcccacgatc
     3301 aggaagaagg cgctcaggtc cctgatgcgg taggtctccg cctcgaacaa cgcgccgccg
     3361 acggcgagat agagcccgcc gaagccgagc gcgaggctca ggaacagcat cgcgagagcg
     3421 cgtttcaagc gctagccggc cgcgccgtca gaagcattat ttagagcgta gcgcaccacg
     3481 tagcgggcga gccgctcggt gtctccggcg aggtccagat ccctgctccg cagcgcaacc
     3541 tcttcgagga gcgccgggct cgcggcgtag cgcgccacgg ctttcgagag cgcctccgac
     3601 gtcctgacgt agtgcccgaa cccttcccgc tcgacgaact ttgccacacc aagttcgttg
     3661 aggcccgcgt aacccgtgac gagcaccgga cgcccgaccg ccagcgcctc gtacaccgag
     3721 gccggtccgg ccttaccgat gaccacgtcg ctcgccgcga cgtggagagc catctcctcg
     3781 acaaagccct cgaccctgag ccccggcgcc cgcagttcct ccagagcgtg ccgcagcttc
     3841 ttgttccggc ccgtaactac gactacctgc ggggcggctt cggagtggag caacgccggg
     3901 actatatggt gcggatgacc accaacccct tcgcctccca gggtgacgag gcaggtaaag
     3961 cggccttcga ggccgaggcg ggcgcgggct tccgccttcg acggagcgtt caggaaaggc
     4021 tggcggaccg ggtagctgac gacggagatc ttatccggcg ggacgcccat gtggatcagg
     4081 tctctgcgca cctccgccgc cgggactacg acgtgctcgg cccgcgggtc ggcccagtag
     4141 gcgcttatgt tgtacggctc ggtcgcgtag gtaaggaccg ggacctggag gccgtagcgg
     4201 tctctggcga gcgcgaggcc cgttgtgagc aggccgtggt tcgagacaac gagcagcgga
     4261 cgttccttct ctagctccgc cgcggccgtg cgggcgaact cgcccacgag acgccgctgt
     4321 ccggcgaccg tcaggcgcgg gaaggagtct atgaggcgct gtccggcgcg ggcgatgaac
     4381 gggtagcgca gcgcgagccg ccagaaggct ttatgccggc ggtcggcgtt caaaacaccc
     4441 aggtcttcca tatagtccga gacgcgcggg gagaactggt cggggtagtg gtattcgatg
     4501 gcctcggaga tcgctcgggc cgtggctacg tgtccgccgc cgacggcgac ggtggcgaac
     4561 aggatccccg gccgcccggc actcacccgg gccacggcgt ccgtccacgc tttagggaaa
     4621 tggagccgga ggcggacctc cgggccggac ttggtgaaag atccaaaata acaggcattc
     4681 cagacgaata tggtatgggc tgtccgagac gatgtctacg gtccggcgtt gcgtcttcgt
     4741 taaatatcgt catgcgactc ctcgccgcgg acggtcctta tcgagcagga ccattgtcgc
     4801 cgcggaagcg ggataggcga gagcgagaag cgtagccaga atgccggagt cgttcagggc
     4861 gcccgaggcg gctgcggcgg ccgtggcgcc gagcattccg gcggcgatgg ctttaccgtc
     4921 cgcggtatcc cggaggcgcc gcagggcggt gaagatcagg gctccaccgg ctgccagcag
     4981 cgcggtccac agcgggttca gcaagtgccg gaggctcagg atgagcttgc gaacggcgat
     5041 ctcgtagaga ccggcctctc cgcccgcagc ccgcgctccg tgcgaggcgt ccgggaagag
     5101 gaagccgctc gacacgaaga gcgccgccgc caggaatagc ccgcctcccg cccatagcgc
     5161 cacgcccgcc atacgcccgc cgcgcagtag ccccaccgtg gcgcccatcc cgaaccccag
     5221 ggcgagggac ccgcccacgt ccgcgcccat cgttggcagg ccaatggcga gcaccgccag
     5281 gactccggcc accgccacag ccgccaatag ccgcggcttc cgcattatca gcaacgccgc
     5341 cccgagcggc agcgctccgg cggcgaccgc cgagtactcg ttgccaatgc catagaaccg
     5401 cgtgccgtag gccgggtcgt ggcccaggat cgagaaccgc atcaaccagc ctccgagggc
     5461 ggcgtcggag atcacgcacg ctgctgtcgc cagggccact accgccaact ctcccagcgg
     5521 gttccgcgag actctccggg acagggcgac aaaagctgcg gcgaacagtg aagccaggat
     5581 cgccacgagc agcgcgttcg tcacgggcac ggccgccgcg gccagcgtcc cgagaggcag
     5641 ggccgtgagg aagagtaccg cgagccggat gcctgcccac cctcgcagga taccggccag
     5701 gacgaacgcc gctaccgaca tcccggacag cagagtccag acaaccgacc gctcctcgtc
     5761 cacgaacgcg agccgatcct ggagccgcga gacctgcgtc atactgccgg gccgggcctc
     5821 ggccgctcgc cccgacatct ccggtggggc ctggacgccc agatgttcga gcagggtggg
     5881 ggcgacatcc tcgctgctca tcaggccagc cgtatgcgtg cccggagagt agaggacacc
     5941 gccctgatct ccggcggcga gtccgaaagg cgtgaggttc gcggagcctg tcggggcgtt
     6001 cggggccgct atagcgacca ccgctccggc ctgccgagcc gcatccgcgg cctctcccgc
     6061 ctcccgtgga tcagatgcct cgaccgcgac cagatccgct ccgtggagga gcgcgtcctt
     6121 cagatgcttc accggctctt tgctgccgta agtgatcggc actctgcctt ttgagtccat
     6181 gacgacgaga gctgcgcgtc cgcccaccga tgcggctcgg gcgccggagt gggccagcgc
     6241 gtctcccagg gcgcccggat gaaccgtcgc cgaaggtccc gtgaaggagc cggggatctt
     6301 acctcgctcc tgtggcaggt tctccggcag gacggaagtg tccgcccgcg cgcccgcgcc
     6361 gaggagcacg tagttcatcc gggggtcctc cggcgcctgt ccttgagagg tcgagaggct
     6421 cgcgactgcg gcgttatcga agaccggcgc gaggcccgga tactctacgc tcttccacgt
     6481 gagcccgctg tccgccagga cgagcacgac cggcgcgtgg tccggctcgc cggacgcggc
     6541 ttgcgccagc gggaacagaa agaccataga taagaccatc gccgctaccg ccgaccgcac
     6601 ggcttcatcc tatcagagag ctgatcgaaa aagaccgttc ccgcctttgt gagcccgttc
     6661 ccggatcatc cctcgcacaa cggcgatact agcctcgtgg gactgagatc atccacactc
     6721 gaccagaact tttcaatcgg cgtcagagca gatgttcctg tttagggagc cgccggatgt
     6781 agctgtccgg cgctttgtcg cgggccagcg cgggcttcca ttctcctaca gtgaagtcgg
     6841 ggcgacgcgc ggcggcgcga cggccggcta cgtcgcggac cgttaccgcg tccggctcgg
     6901 tgaaggggag gagaccttcg agcgggcggt cgcggcgctg cgatcctgga gtatgttcga
     6961 cctcgggtgg gcctgtctga tccccgacga tgctcctatc gaacggggcg ttaccgtcgc
     7021 cgtcctcgcc cggcacctcg gcttgtggtc gctgaacgcg gcccgcatcg tctacgtggt
     7081 cgaggattcc ggcagcgtgg agcgccgcgg cttcgcctac gggaccctcc ccggacacgc
     7141 cgcccgcggc gaggagcgct tctgcgtgga gtgggaccgc gcgtcggact ccgtccatta
     7201 cgacgtgctc gcctttagtc gtcccggcca cccgctcgcc tggctcggct acccgttcgt
     7261 ccggcggctc cagcggcgct tcgccagaga ctccagcgcc gccatgttag cggccgcgag
     7321 gggcggcccc tgaatggctc tccgctgatt ccgccgaacc tgctagactg cccggcggta
     7381 tgaacgcgac gacgcacgcc atattcgggg tcggggctct ggccggagcg gcgctcctga
     7441 cggggatgga gccgcccctg tacgcctacc ccatggcggt ggtcgccgcg tggctgccgg
     7501 acgtggataa cccccgcagc accctgggta acggcctgag ccgccacaag aacccgttgc
     7561 tgaacgcggt aacgcgcccc attagctggg cattgaggac atcctcgttc ttcctgatcc
     7621 gcgtggtggg acaccgcacg ctgacccact cgctcctggg cctcgctctc tacgcgctgg
     7681 tggcgtggct ccttctggga ggctacccga acctgttcct cgcgctcctc gtgggagctg
     7741 cctctcacat cgtcgcggac gctctaaaca cctggggcgt accgctcctg tggccgctga
     7801 agatacagtt ccggttgttg ccggggggtg tcaggtccgg gggcgcggcg gagttcgtag
     7861 tggcgctcgt cgtgctgggg ctgacggcct acgcgctgtt cgaggtacac ccggcgctac
     7921 gaggtttcct cggtctcctc cagccactct agggtcttcc ggctctccgg gtgcacgtca
     7981 cgcccgcgct gctcgttgta tgagatcgcg cggcgcaggg cctcccgcgc agccgcgcga
     8041 aggtctttcg cggcgaggtc gcgcaggtgg ccaacgctcg gatagtcgcg ggcgggctct
     8101 atcttgtccg cgacgtagac ggcgagggcg aggtaagaca tccccggaac gccggtggtg
     8161 tggacccgga tcgcctccag cacttcctca tcctcgacac cgagcttgcg ccttgcaagc
     8221 tccgccgcaa cgggaccgtg cagcagcttc gggttctccc gctcgaagtc tccgacgggg
     8281 aggtcccagt ccctcgccag ccgcaggtac tctccggcgg acatctcgcg ggcggcgtcg
     8341 tggaggagcg cggccaggcg tgtccactcc tcgttcagag cgtgagtacg cgccaggcgc
     8401 tccgccgtct cggtcacgcg gatggtgtga gcgtagcgct tctcggagag gcggtcgcgg
     8461 gcgaaggcgt cggcggtctc ggaggataca taattcggtt cacggtcttc cattcggtgc
     8521 attgtagaat acgcgcgtgg atagagaaca gaaaaacata gagagcgcgg agatcattac
     8581 catcgggacc gagatgttgc tcggtgaact ggtggacact aactcggcct ggattagcca
     8641 gaggctcgcc gagctgggcg tcgggatcta ccgccacacc accatcgggg ataaccgcga
     8701 ccgcatcgtt accgagatga aggaagccgc ctcgcgctcc gacctcgtca taaccaccgg
     8761 cggtctcggc ccgacctccg acgacctgac gaacgagtgt ctggcgctcg cggcgggccg
     8821 ggagatggtc gagtatcagg aggcgcgcga ccacgtggac accatgttcg cccgcttcgg
     8881 ccgcacgcca acgccgtcca actacaagca ggcgctcttc ccggagggga ccacgctcat
     8941 cccgaaccct ctcggcaccg cgccgggcgc gctactcgaa gtagacggcg cgctcttcgc
     9001 caccctgccg ggagtgccgg gcgagatgaa gggcatgttc accgagactc tggcgcccct
     9061 gatccgggcg cgcagcgacg gctccatcgt ctcccgcacg ctctggttta ccggcatcgg
     9121 cgagtcggcc ctcgcggagg aggtccagga cttcctcgac gccacggacc cgaccgtcgc
     9181 cccgctcgcc ggacagggca aggtgcgcct ccgcatcacc acccgcgccg ccacccccga
     9241 agccgccgaa gagaagatag cgcccgtcga gaaagagatc ctgtcccgcc tcgacgagta
     9301 ctacttcggc caggacgagg agaccctgga gagcgccgtc gggcgtctgc tccgggagca
     9361 cggcgcgacc ctcgccgtcg ccgagagctg caccggcggc ctcatcgcca agcgcctcac
     9421 cgacaccccc ggctcctcgg cgtacttcga ggaggggttg gttacctact cgaacgaggc
     9481 caaggaacgt cacctcggcg tcccgcggga gatgctcgtc cagcacggcg cggtctccga
     9541 gcaggtggcg ggtgcgatgg ccgagggcgt ccgaaaagcc gccggcacgg actacgggct
     9601 ctccgtgacc ggggtcgccg gtccggatgg ggggaccgag gagaagttcg tagggctcgt
     9661 ctacgtcggg gtctccgatg cgcagaagac cgaggctgag aggttggacc tgtcggcgtg
     9721 ggcgcggtcc agggatgcga tccgggagcg cagcgcgaac cgggcgctgg acctcttgcg
     9781 gctgcggatc gaagagaaaa aataaatctt aagaattact cttg
//
LOCUS       DASSMB010000034         3922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1147644, whole genome shotgun sequence.
ACCESSION   DASSMB010000034 DASSMB010000000
VERSION     DASSMB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147644"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..834)
                     /locus_tag="VFJ72_02465"
     CDS             complement(247..834)
                     /locus_tag="VFJ72_02465"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7478360.1"
                     /translation="MPAEKEHMAREFLERMCEEVWGRGNLDVIDDLFAPACVVHDHHT
                     PPGGEQRSPERLKSSAAKFREAFPDLHVDIGAIVVSSEGVEARISYHYRLTATHLGRL
                     HAIAPTGRQVNITGMSMMRLAGDRIVESWESSDTLTMMRQLDLIVPKPQKAPGLFSRP
                     APPRPSRRSSLSNGNGWNDLAMLAAFLLGWSIGDD"
     gene            complement(859..1722)
                     /locus_tag="VFJ72_02470"
     CDS             complement(859..1722)
                     /locus_tag="VFJ72_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478361.1"
                     /translation="MKEYSNMGHVMDARLAAWVSSVPPCAAGYYLHSGAELPEPEERP
                     ISNRPDWRDSAAAAVLRECYRRAMFDPHLERVEAAALAAMPAPDDEEEEDELSLIPRM
                     FRTPTDRERWEMAVSHTAARVSRLLRYGPTAGMPGGEAVFAGEMEAEVRGLRLRQRVD
                     LLWRHPGGALEAVLVFEDSRGGNAPPPVEQDWRCILAAAVVSALYGAVPRVHALWISA
                     GAAQVVNYPVGHVEEKVLGLADAVFRAGKPDVCGETISYPLVPEAIEPLRHGKWPRDP
                     RPFPPGMPPRR"
     gene            complement(1850..2170)
                     /locus_tag="VFJ72_02475"
     CDS             complement(1850..2170)
                     /locus_tag="VFJ72_02475"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HET7478362.1"
                     /translation="MRMWLDDLRPPPRGWVWVRTVEEAIPLLAAGDVVEASMDHDLGE
                     GVEEGYALVLWMAEHDIWPGHALEVHSANPVGAERMCGVIERYGPYRRVMGTRRFARP
                     PIRE"
     gene            complement(2223..2405)
                     /locus_tag="VFJ72_02480"
     CDS             complement(2223..2405)
                     /locus_tag="VFJ72_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478363.1"
                     /translation="MDIGEKVKEVILEPLAEPGRPVREPVREPAPEEAPATEPDRERD
                     PERRGEPAGPRRSTRR"
     gene            complement(2419..3399)
                     /locus_tag="VFJ72_02485"
     CDS             complement(2419..3399)
                     /locus_tag="VFJ72_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478364.1"
                     /translation="MSHRKTIVGILSAIVALYLLANHTGLFFTLALAAGVLWFIGRRE
                     PAAAPPGFTAAPAAAAPGNHYSPRKDSRGPGDVASDAGSAAVLSRMLQDLCRDDSSEG
                     YDPRPESADWHGVLPPLTSGATTDPAPDYAQALIGYREWAFVPRIETTSGFRAAPDTL
                     FSLTRQANGTGGSPLAQRRERYHEWPLDGPHEARCGVSGLLPSRGNCPSCTCGFYAWS
                     EELWPICRPGQTLYGTEPFSWAVPGIIAGWGKVSIHQRGFRCQYARVVALLDPGAFAF
                     DDPGEPGWPDPKTAYLDLIGRKGVPLISPDEMLDPDLPARFGLLRASQIT"
     gene            complement(3519..>3922)
                     /locus_tag="VFJ72_02490"
     CDS             complement(3519..>3922)
                     /locus_tag="VFJ72_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478365.1"
                     /translation="DWDIFDETWLLMWAEAHNIDPDSLRAERDTGETPKEGFYLPEGL
                     LSFGTMEFVDDANAIGIPGVSVAKNSDNKETLEVRGRDTLEKLRDLLSGRYRIVEKSR
                     AADYLELPDLRETKVMLNEHREAARKEHRNE"
BASE COUNT          589 a         1316 c         1294 g          723 t
ORIGIN      
        1 aggagttggc gcgtcttttt tctttcgttc ttgttcaact tacggctact ccttcccccg
       61 gagcgggacc ctggtctccg catctcggat catgccacca gccatgctcg cctacaggcg
      121 cgtagacttt tcatgacgac ctggatcccg caccccattc atcaccccca cctctcaaac
      181 gttagtgccg ttcattgtaa tcgagctatc gccaaaggtc gaaacgggtg cttcgccgca
      241 cgtcattcag tcgtccccga tggaccagcc gaggaggaag gcggcgagca tcgccaggtc
      301 gttccagccg ttgccgttgg agaggctgct ccgtctcgag ggacgaggcg gggcgggacg
      361 gctgaagagg ccgggggcct tctggggctt cggaacgatg aggtcgagct ggcgcatcat
      421 ggtgagggtg tcgctgctct cccagctctc gacgatcctg tctcccgcga ggcgcatcat
      481 gctcattccc gtgatgttta cctgccggcc ggtcggggcg atggcgtgca ggcgtcccag
      541 gtgcgtcgcg gtgagcctgt agtggtagga gatcctggcc tctacgccct cgctcgacac
      601 gacgatggcg ccgatgtcca cgtgaaggtc ggggaacgcc tcgcggaact tcgccgcgct
      661 gctctttagc cgttcggggc tcctctgctc tccaccggga ggcgtatggt gatcgtggac
      721 gacgcaggcg ggagcgaaga ggtcgtcgat cacgtccagg ttcccccggc cccagacctc
      781 ctcgcacatg cgctccagga actcgcgggc catgtgttct ttctctgctg gcatggatac
      841 gctcctctct ccgacgcgct aacggcgggg cggcattccg ggtgggaagg gtcttggatc
      901 cctgggccac ttgccgtgcc gcagaggctc gatggcctcg gggacgagag ggtagctgat
      961 cgtctcgccg cagacgtcgg gcttcccggc cctaaagacg gcatccgcca ggcctaggac
     1021 cttctcctcg acgtgtccga cggggtagtt gaccacctgg gccgcgccgg cggagatcca
     1081 gagcgcgtgt accctgggga cggccccgta cagggctgag acgaccgccg cggccaggat
     1141 gcagcgccag tcctgctcca ccggcggcgg agcattcccg ccgcgcgagt cctcgaagac
     1201 gagcacggcc tcaagagctc cgccagggtg gcgccagagc aggtccaccc gctgccgcag
     1261 acgcaggccg cggacctcgg cctccatctc gcccgcgaag acggcctcgc cgcccggcat
     1321 acccgccgtg ggaccgtagc gcaggagccg gctcaccctg gccgcggtgt gggagaccgc
     1381 catctcccag cgttcccggt cggtgggcgt gcggaacatg cgagggataa gagagagctc
     1441 gtcctcctct tcttcatcgt cgggcgcggg catcgccgcg agggcggcag cctccaccct
     1501 ttcgaggtgc gggtcgaaca tggccctccg gtagcactcg cgcagcacgg cggccgcggc
     1561 ggagtcccgc cagtcgggtc tgttgctgat cggcctctcc tccggctccg gcagctcggc
     1621 gcccgagtgc agatagtagc ccgcggcgca gggcgggacc gaggagaccc aggccgccag
     1681 ccgcgcgtcc atgacatgtc ccatgttcga gtattctttc atcttcggct ccttccgcgt
     1741 ggtgtgtcct attcataact ttacggtaga gggtggatgg aacttcgcgg cggggtccac
     1801 gcctgggagc ggcgcccgaa ctgcctatga gccgtagatc tccagcaccc tattctcgga
     1861 tcggcgggcg tgcgaagcgc cgcgtgccca ttactctgcg gtacggtccg tagcgttcga
     1921 tcaccccgca catcctctcc gcgcccaccg ggttcgcgga gtggacctcc aacgcgtggc
     1981 cgggccatat gtcgtgttcg gccatccaca ggacgagggc gtaaccttcc tcgacgccct
     2041 cgcctaggtc gtgatccatc gaggcttcca cgacgtctcc ggcggcaagc aggggaatgg
     2101 cttcttcaac tgtcctgacc cacacccagc cccgtggcgg cgggcgcagg tcgtcgagcc
     2161 acatcctcat ccgcagatcc ggggagaaag gcggaaatcc aggcgatcct ggttcttcgc
     2221 gtttaccggc gcgtgcttcg tcgcggtccg gccggttctc cgcggcgctc cgggtcccgc
     2281 tccctgtcgg gctctgtcgc gggagcttct tcgggggctg gctccctgac gggctccctg
     2341 acgggccggc ccggctccgc gaggggttcg aggattacct ctttcacctt ttcgccgatg
     2401 tccactacgc tctacctcct aggttatctg ggacgctctc aggagcccga acctggcggg
     2461 cagatctgga tccaacatct catccgggga gatcaggggt acgccctttc gcccgatgag
     2521 gtccaggtaa gcggtcttcg ggtccggcca tccgggctcg ccggggtcat cgaaggcgaa
     2581 ggcgccgggg tccagcaggg ccaccaccct cgcgtactgg caccggaacc cccgctggtg
     2641 gatgctcacc ttcccccacc cggcgattat gcccggcacc gcccacgaga aaggctccgt
     2701 gccgtagagg gtctggcccg gacggcagat gggccacagt tcctccgacc aggcgtagaa
     2761 gccgcaggtg caggacgggc agttgccgcg cgaggggagg agacccgaga cgccgcaccg
     2821 ggcctcgtgc ggcccgtcca gcggccactc gtggtagcgc tcccgccgct gcgcgagcgg
     2881 agaaccgccg gtcccgttgg cctgcctggt caggctgaag agcgtgtcgg gggccgcgcg
     2941 gaagccggaa gtcgtctcta tgcggggcac gaaggcccac tcccggtagc cgatgagcgc
     3001 ctgggcgtag tccggcgccg gatcggtggt cgcgccggag gtcaggggcg ggaggacgcc
     3061 gtgccagtcg gcgctctccg gtcgggggtc gtacccttcc gaagagtcgt cccggcacag
     3121 gtcctgcagc atcctggaga gtacagcggc ggagcccgcg tcactggcaa cgtctcccgg
     3181 accgcggctg tccttacgag gactgtagtg gttcccggga gccgcggccg ccggagcagc
     3241 cgtaaagccg ggaggcgcgg ctgctggctc tcgccgcccg atgaaccaca ggacaccggc
     3301 agcgagcgcc agcgtgaaga acagccccgt gtggttcgcc agcaggtaca gggccacgat
     3361 ggcggacaag atcccgacga tggttttcct atgactcaca tccagctccc ttctttcggc
     3421 ctgtaccctg aaagttagca gccggggtgg atggaacttc tttgcgcccg tccgggacca
     3481 cggagcgcag gaagctggag actgcctact ccgctggttt actcgtttcg atgttccttc
     3541 ctggccgctt cgcggtgctc gttcagcatc accttggtct cccgcaagtc tggaagttcc
     3601 agatagtccg cggcgcggct cttctctacg atcctgtacc gtccggagag gaggtctcgc
     3661 agcttctcta aagtgtcccg gccccggacc tccagagtct ctttgttatc gctgtttttg
     3721 gcgacgctca caccggggat gccgatggcg ttcgcgtcgt ccacgaactc catggtgccg
     3781 aaggagagaa ggccttcggg aaggtagaag ccttctttcg gggtctcgcc tgtgtctcgt
     3841 tcggccctga gcgagtcggg gtcgatattg tgggcctccg cccacatgag gagccaggtc
     3901 tcgtcaaata tgtcccagtc ct
//
LOCUS       DASSMB010000035        16436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_768856, whole genome shotgun sequence.
ACCESSION   DASSMB010000035 DASSMB010000000
VERSION     DASSMB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16436
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768856"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /gene="sufD"
                     /locus_tag="VFJ72_02495"
     CDS             <1..933
                     /gene="sufD"
                     /locus_tag="VFJ72_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET7478366.1"
                     /translation="LFKLVPESYDKFAALSAAAFSGGSFLYVPRGVDVEVPIQSYRWL
                     DVIGSIMPRTLVIVEEGSSVTYIDEYASADGEDPAFSNGAVELFVGEGSSLRYVSLQN
                     WERNVLHFNTIRSQTEKDATINSLVVSLGSQLSRTNVEAGLTASGGDSEMLGLYFADQ
                     NQVLDHHTLQDHISPNAHSDLLYKGALRDESLAVFSGLIRVEPGAQKTDAYQTNRNLI
                     LGTDDAFAVSLPNLEIMADDVKCSHGSTTGQVDEVELFYLMSRGIPRREAEKLVVFGF
                     FGEVTSKIPLKGLKEKLDRAIEGKIGLGFEERVA"
     gene            937..1290
                     /locus_tag="VFJ72_02500"
     CDS             937..1290
                     /locus_tag="VFJ72_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET7478367.1"
                     /translation="MAEFHKLAAADEVSPGEIKQYKVEDRFVALCNVDGEFHAFEDIC
                     THQFTHLSEGEFTDSEVKCPLHGAKFDVKTGAAKSLPAVKPVPKHEVKVEDRYVYVSL
                     NPKRVKVGGRGGRRR"
     gene            1293..2522
                     /locus_tag="VFJ72_02505"
     CDS             1293..2522
                     /locus_tag="VFJ72_02505"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET7478368.1"
                     /translation="MYDVEKVRTDFPILERRIGENKLVYLDNAATSQKPRQVIETLTE
                     YYEQHNANIHRAVHTLAEEATTAYEESREKVARFLGVPDSRALIFTRGTTESINLVAH
                     AWGRKFLREGDEVVLTVAEHHSNLVPWQLAARATGARLRFIPVLDDGTLDMEEAERLI
                     GPRTKLVSVIHASNVLGTINPVGRLADLAHENGALMLVDGAQSAPHMPVDLEDLGCDF
                     FACSGHKMLGPTGVGVLWGRPELLEEMEPFLGGGEMIREVHLDYSTWNDLPYKFEAGT
                     MNIAQAIGLGAAVDYLSALGMENVREHERRLGEYAYRRLSEVEDIIIYGPQEDRTGLV
                     SFSLPDVHPHDLSQLLNDEGIAIRSGHHCAQPLMRRLDVSATARASFYLYNTEDEVDA
                     LIEALAHAREFFRPFAI"
     gene            2525..2938
                     /locus_tag="VFJ72_02510"
     CDS             2525..2938
                     /locus_tag="VFJ72_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET7478369.1"
                     /translation="MMRELYTQVIMDHYQRPRNRGEIEDADLEEHLLNPLCGDEVTVY
                     ARFDGDKVADVKFSGRGCSISQASASMMTERILGKSREEAEAQIESFKSMMIGEREFP
                     ETDDLAALKGVIQYPSRIKCATLAWTAFQRGLEDR"
     gene            complement(3073..4218)
                     /locus_tag="VFJ72_02515"
     CDS             complement(3073..4218)
                     /locus_tag="VFJ72_02515"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET7478370.1"
                     /translation="MEWLLLPVGVILILTGLTDVFFTVLHYDGFGFLSSRLYNRLFHV
                     VSFVTRPLPGRYRALGLSLAAPLMVPVTIFVWILLVSSGYALIYYAGMDAHTFYFSNS
                     SLEPSLLEAFYLSGTAISTLGFGDVTPLSAFYQLLTVSEALIGFGILTLAITYVLGVY
                     GVLQQLGILAAGLYHQAQDTSEPLDILIPHFPNGEHRGLETHLMAVHRELVEIYEGIR
                     RYPIVYYYHSRRAYRSLPYTFRMLGGMAAALRWGLPKDHPGAQTPWLPTLITGLDNVT
                     SYLDERFLSEHLEESPEPVQFETFEAAYSRGKEPADKWLSRFIQITSYMCEMVLLDSL
                     PDAREAYERYTEWLPFAHRNREFFEASARDLGYELEDLDVAPGKRLF"
     gene            complement(4248..4556)
                     /locus_tag="VFJ72_02520"
     CDS             complement(4248..4556)
                     /locus_tag="VFJ72_02520"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET7478371.1"
                     /translation="MLIAPEESKDTIERFREKVEQNPVEYVAQEVVEFSTHVLCETEG
                     DELLLRDTHADYRVHALAPEAETAEVVPGAMTRVAAPGSKLVNISSGGKTKDTWVLRA
                     "
     gene            complement(4872..5279)
                     /locus_tag="VFJ72_02525"
     CDS             complement(4872..5279)
                     /locus_tag="VFJ72_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478372.1"
                     /translation="MKPSNRLPKLKIFRSPPKTFFEEDEEIDAPPKGPLLPRPLFYAA
                     LAAFGVSLMLAADWFGLLLLNSQGVFLQIGSLNMLRVTAALMSGISVPSVFLMLHYGR
                     VYSYTSEENVFFAITFSISFMLGLPCGLVGVFA"
     gene            complement(5320..6129)
                     /locus_tag="VFJ72_02530"
     CDS             complement(5320..6129)
                     /locus_tag="VFJ72_02530"
                     /inference="COORDINATES: protein motif:HMM:NF009499.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7478373.1"
                     /translation="MSEETRRSTARDDLLRGRVALVTGAGRRRGIGTAICRSLALCGA
                     DIVFSYWRAYDREMPWSSNEDEPDTLLEELRATGVRAEGVEVDLSRPDSPERLLDSAT
                     EKLGTPSILINNAAHSTRDGFENLDAAMLDAHYAVNVRAMALLCVEFARRYPGGPGGR
                     IVNLTSGQSLGPMVGELAYAATKGAIEAFTVTLAAEVGQKGITVNAVNPGPTNTGWMT
                     EELKRDLAPKFALGRVGEPEDAARLVTFLASDEAAWITGQVIHSEGGFLRS"
     gene            6371..7138
                     /locus_tag="VFJ72_02535"
     CDS             6371..7138
                     /locus_tag="VFJ72_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET7478374.1"
                     /translation="MSTQPKSYLLDMDGVLMRGGEPIPGAPEFIARLIERQAKFLILT
                     NNSLYTPRDLQLRLRRRGLEVPVESIYTSAMATAEFLDSQTPNGTAFVIGEAGLTTAL
                     HETGYSLVDTDPDYVVLGETATLSFQRITQAVRMILAGARFIATNPDTSGPSEGGIIP
                     ATGAIAALISSATGVMPYFVGKPNPLMVRTALNMIDAHSEDSVMIGDNKDTDVVMGIE
                     SGLETILVLTGVTTLGEVERHPYRPTRIAESVAEIMP"
     gene            7192..7440
                     /locus_tag="VFJ72_02540"
     CDS             7192..7440
                     /locus_tag="VFJ72_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478375.1"
                     /translation="MTVFRYGRAYYDEGEFVRRKDAMPGDDLDPVVRVVENLGTIEIP
                     CGKVDCRSNCLEYLVRSVRRAEDYNLAECKLSPLVSII"
     gene            complement(7453..8004)
                     /locus_tag="VFJ72_02545"
     CDS             complement(7453..8004)
                     /locus_tag="VFJ72_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478376.1"
                     /translation="MFLRLMGVGALYGFGFALIKSLFPSPVILLAFSDAPTGEGSPAA
                     LMLVYMGTGLVAGFVAAFVFGGILLARRGATDESSSYYGPRFALSMILALLMGLISGL
                     LTIVAYASGILPAGGVLDPLKLIRASNFSPGTPLLVAWTIARDLLPAGLTGLFLSPVG
                     GGMLMRLYAADRPPVQKTYEDFE"
     gene            complement(8004..8480)
                     /locus_tag="VFJ72_02550"
     CDS             complement(8004..8480)
                     /locus_tag="VFJ72_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478377.1"
                     /translation="MTTAVRNRLILLGALWGVVLAAVPALVMSRPFHLSGFLVAAVAC
                     SVVSGSVGTLVAGRRAIRPGKKRGAVAAALGTGLFQGLAGGALSAALIWALMAVTLSG
                     FTLGNPVQLATLMSPRVFLGGFFVALSVFVYVLAGGLLLGPVFGTLINSLVRKGDA"
     gene            8751..9137
                     /locus_tag="VFJ72_02555"
     CDS             8751..9137
                     /locus_tag="VFJ72_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7478378.1"
                     /translation="MLKVKLDHCVIHVSEWERSNRFYEEVMGAEVVPAGERWSYRFGD
                     QQLNLHGPGVDPTPVARLPVQPGNSDLCFEWPGPISEARKHLEQHGVEVELGPVERNG
                     ARGSGTSLYFRDPDGSLLEFISYSGG"
     gene            9139..10317
                     /gene="queG"
                     /locus_tag="VFJ72_02560"
     CDS             9139..10317
                     /gene="queG"
                     /locus_tag="VFJ72_02560"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET7478379.1"
                     /translation="MAQRRTDTHPDPAEARRVLKERAREAGFDLVGVTSAEPLREGGE
                     RLREWQEARMAADMCYMQRPVELLSDPKKLQKSARSVVSLGVSYYPGDHPENADGGGR
                     VARYAWGRDYHVVIKERLYRLREELEKDLGVKVKARAFTDAVPLLERSAAQRAGLGFF
                     GRNSCLINGDVGSYFFIADLTLDLDLPIDEHGTGTCGRCSRCMDRCPTGAIKAPGVVD
                     ARLCISYLTIENRGEIPRELREKVGDWAFGCDVCQEVCPYNKTKAARSRWPEFSEEAG
                     AGPYLDIVEVLEIRTDEEFEQRFAGTPLTRPGRAGLLRNCCVAAGNLKQERAVPALLR
                     CLAEDESELVRGHAAWALGEIGGSEKELREALATERDEGCRGEIRQALGDGTGSAPVG
                     "
     gene            10330..11166
                     /locus_tag="VFJ72_02565"
     CDS             10330..11166
                     /locus_tag="VFJ72_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7478380.1"
                     /translation="MARQINYYEILGVSREASQTEIRNAYRTLAKQRHPDHPGGSADD
                     FSLLQEAHATLSDPNRRRHHDEALDLAFAADQLADLDFDSLGDELSAKRRERESSGPS
                     FGERLRGRFGRKEEDPFEQLRRETRESGRSSGRESGRRGRGRYREERAARWYEPHDFD
                     PEPIGWKTGAISFVAAFLAFIAVGQIGLWAINPASAGSLAGISVLASFMPILYTLVGL
                     VASYFAYRAAGYGAVGLVFVAALVVGGRGGPTGLLQFGTLGIVLLLVVIWLGKRRDRP
                     GR"
     gene            11271..12929
                     /locus_tag="VFJ72_02570"
     CDS             11271..12929
                     /locus_tag="VFJ72_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HET7478381.1"
                     /translation="MISTTEIQSFVSSFETRVAPVEKAFGEAWWNLATTGTEEAQEEL
                     VRAGNAYDALFADAEEFERMRGWYEERGSLQDPLLRRQVEVLYRAFAGKQGDREILRN
                     IQELEAEANATYSNHRGVVDGREIGENEVREVLRSSGDDRLRREAWEASKTVGREVEA
                     TVRELARLRNRLAREAGYPDHYHRSLDLQEIGAADLARLMDELESVTEAPFRDLKSRL
                     DTSVRERFGVESVMPWHLPDPFFQSCKQDPSAFPRPDSRGGSGSASGDGNGVDVDSFF
                     RDKDLEALTRKTYDNMGLEVRDVMAKSDLYEREGKSQHAFCLGVGRDYPYDVRVLANV
                     QPDSYWMDTMLHEFGHAVYDKHINPGLPYFLRSIAHINTTEAIALMMGSLADDPEWLS
                     DIAGVSEAELSENREYLHGRDRADKLVFTRWALVMFHFEQALYADPGREDLNTVWWDL
                     VERIQLVERPPDRDEPDWAAKIHVATAPVYYHNYVLGHLTAAQLRHYLETYITHGPFF
                     ESEVAGRYLLEAVFGPGARDDWQTTVLRATGEKLNPRYFVESLG"
     gene            complement(12926..13906)
                     /locus_tag="VFJ72_02575"
     CDS             complement(12926..13906)
                     /locus_tag="VFJ72_02575"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET7478382.1"
                     /translation="MRVRMVLPLVLICLVFIAGCGSAGRGDGADRDGAGTETMGGTSA
                     HTTAGETTAAETSTEATVGAEKEEPGSTTATDQTGTGGKAESSGEGPFIASPESSGGE
                     GFQADSILAVRYGDHGGYERVVLDLGTGSRPAGSIPEWKLTSPTGDGLLRVSLPSVSA
                     TQVSDGKLGGKLLKDFHAVRGPDGGMFVDIVADEAFTYRVMELTDPARLVVDFKPSEM
                     PLDMPLPAEGDKTVVTQPREGERVGSSLTVSGYSRNFEGTNTIVLTDSGGEVIARKTV
                     QANDWAVTWGYFETTLKAPSFSGRGTLRVGAESARDGTFEGVEVPVRGSG"
     gene            14060..15793
                     /locus_tag="VFJ72_02580"
     CDS             14060..15793
                     /locus_tag="VFJ72_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478383.1"
                     /translation="MKHRPRHDEPRAAFRASRVSEHLFALALYTALAVVFTYPLAFNM
                     DRVDGAGDPAVMVWSMAWIQHALTTGASLYDANIFYPTQNALAYTDLLLPSAVITTPL
                     YLATDNPLLSFNVVLLLTYVLSGYATFLLVRRLLGGRPYALLAALFAGAVYALCPYRY
                     GHITQLNTMTTYWLPLILLFMHRYLEDGRRFRDMLLVGLFFTLNVLSGLYYGVFAVLM
                     MASFYLLWSFMHREPPKVRDLALGIPVFAAFGAVLALVLGPYIALSGASDHARDISTV
                     AGGSFVPAAILTSVPESWFLGWTPEVLGITHENRKPVYELTLYPGLAVTALAVYGLLR
                     RNRVAIHSALYALLGLVIFIFSCGPAIPVGDSFIPLPYYLLYEFVPGFGNLRVPARMW
                     AIVMLCIAVLAGFGLRALMQRLGGKKALLALAVISLFTAFEFVPKLPIDRYIDRGPAT
                     IEPAYRYLADRAPGAVVAEVPFASPADAFRETPRMYRSTFGWWRLVNGYASYFPDGYT
                     ETRDALNTLPSPESLKELRRLGVEYVVVHPDQYDVDGMDGEEMSRKVGREPSLQRVAG
                     DGETVLFRVKP"
     gene            15790..>16436
                     /locus_tag="VFJ72_02585"
     CDS             15790..>16436
                     /locus_tag="VFJ72_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7478384.1"
                     /translation="MISLHDIQTAQRRVRGVALRTPLVSCPGGEEDRVLYFKPENLQP
                     TGAFKLRGAYNKISSLTEEERRLGVVAHSSGNHAQAVAYAARALGVRAVIVMPRGAPK
                     VKLDATRAHEAEVVVVGDDSTERAHRAEQLAEEFGYTPVPPYDDETLIAGQGTVGLEI
                     LEDLPDVETVLVPVSGGGLISGVAAALKLNRPDINAIGVEPELAADARASLRSGR"
BASE COUNT         3021 a         5232 c         5372 g         2811 t
ORIGIN      
        1 ctcttcaagc tcgtgccgga gagctacgac aagttcgctg cgctctcggc ggcggctttc
       61 agtggcgggt ctttcctgta cgtgccgcgc ggcgtggacg tggaggtccc gatccagagc
      121 taccgctggc tggacgtgat cggctctata atgccgcgca ccctcgtcat cgtagaggag
      181 ggctcgtcgg tgacgtacat cgacgagtac gcgagcgcgg acggcgagga cccggcgttc
      241 tccaacggcg cggtcgagct gttcgtgggg gaaggctcgt cgctgcgcta cgtgtcgctt
      301 cagaactggg aacggaacgt tctgcacttc aacaccatcc gctcgcagac ggagaaggac
      361 gcgacgatca acagcctcgt cgtctccctg ggctcgcagc tatcccgcac gaacgtggag
      421 gcgggcctga cggcctccgg cggcgactcg gagatgctgg ggctgtactt cgccgaccag
      481 aaccaggtcc tcgaccatca cacgctccag gaccatatct cccctaatgc tcactcggac
      541 ctgctgtata agggcgctct gcgcgacgag tcgctcgcgg tcttctccgg tctaatccgg
      601 gtggagccgg gggcgcagaa gacggacgct taccagacga accggaacct gatcctcggg
      661 acggacgacg ctttcgcggt gagtttgccg aacctggaga tcatggcgga cgacgtgaag
      721 tgctcgcacg gttcgactac tgggcaggtg gacgaggtgg agcttttcta cctgatgagc
      781 cggggcatcc cgcggcggga ggcggagaag ctggtcgtgt tcgggttctt cggcgaggtt
      841 acgagcaaga tccccttgaa gggcctcaag gagaagctcg accgggctat cgagggtaag
      901 atcgggctgg gcttcgagga gcgggtagct taaacgatgg cggagttcca caagctggcg
      961 gcggcggacg aggtttcgcc cggagagatc aagcagtaca aggtcgagga ccgcttcgtg
     1021 gccctgtgca acgtggacgg cgagttccac gccttcgagg acatttgcac ccaccagttc
     1081 acgcatctga gcgagggcga gttcaccgat tcggaggtga aatgtcccct gcatggcgcg
     1141 aagttcgacg tgaagaccgg cgccgcgaag agcctgcccg ccgtcaagcc cgtcccgaag
     1201 cacgaggtga aggtcgagga cagatacgtg tatgtctccc tcaaccccaa gcgcgtgaag
     1261 gtcggcggca gaggcggccg caggaggtag ccgtgtacga cgtggagaag gttcggacag
     1321 acttccccat cctggagcgc aggatcgggg agaataagct cgtctacctg gataacgcag
     1381 cgacctccca gaagccgcgg caggtcatcg agacgctcac cgaatactac gagcaacata
     1441 acgccaacat ccaccgggcc gtccacactc tcgccgagga ggcgacgacg gcatatgagg
     1501 agtcacggga gaaagtcgcc cggttcctgg gcgtgccgga tagccgcgct ctgatcttca
     1561 cccgcggcac gaccgagtcc atcaacctcg tcgcccacgc ctggggccgc aaattcctga
     1621 gagagggaga cgaggtggtc ctcacggtgg ccgagcacca ttcgaacctc gttccctggc
     1681 aactcgccgc gcgggcaacc ggcgcgaggc tacgcttcat cccggtcctg gatgacggaa
     1741 cgctcgatat ggaggaggcg gagcgcctga tcggcccgcg gacgaagctg gtctcggtta
     1801 tccacgcctc taacgtgctc gggaccatca acccggtcgg gaggctggcg gacctggcgc
     1861 acgagaacgg cgcgttgatg ctggtggatg gagcgcagag cgcgccgcac atgccagtag
     1921 acttggagga tttaggctgc gacttcttcg cgtgcagcgg gcacaagatg ctcggtccga
     1981 ccggcgtcgg agtgctgtgg gggaggccgg agctactgga agagatggag ccgttcctgg
     2041 gcggtggaga gatgatccgg gaggtccacc tggactactc gacctggaac gacctgccct
     2101 acaagttcga ggccgggacg atgaacatcg cccaggctat cggcctcggc gcggcggtcg
     2161 attacttgag cgctctcggc atggaaaacg tgcgcgagca cgagcggcgg ctgggggaat
     2221 acgcttaccg gcggctctcg gaggtcgagg acattatcat ctatggtcca caagaagacc
     2281 ggacggggct cgtctccttc tcgctgccgg acgtccaccc gcacgacctc tcccagctac
     2341 taaacgacga ggggatcgcc ataaggagcg gccaccactg cgcccagcct ctgatgcgcc
     2401 ggctcgatgt gtcagcgacg gcccgagcga gcttctacct gtacaacacc gaggatgagg
     2461 tggacgcgct gatcgaggcg ctcgcccacg cccgcgagtt cttcaggccg ttcgcgatat
     2521 gagcatgatg cgcgagctat acacgcaagt gatcatggac cactaccagc gtcccagaaa
     2581 ccggggcgag atagaagatg cggatcttga ggagcacctc ctcaacccgc tctgcggcga
     2641 cgaggtgacg gtctacgcca ggttcgacgg cgataaggtc gcggacgtga agttcagcgg
     2701 tcggggatgc tccatctccc aggcttcggc ctcgatgatg accgaacgga tcctcggcaa
     2761 gagccgcgag gaagcggagg cccagatcga gagcttcaag tccatgatga tcggggagag
     2821 agagttcccc gagacggacg acctggcggc gctgaagggc gtgatccagt acccctcccg
     2881 catcaagtgc gctaccctcg cctggaccgc cttccagcgc ggtctcgaag accggtaagt
     2941 ccggcttacc gacccgctcc taactttctt cgccgcatga ttctcgtcat ggccggcagg
     3001 ccgccgcgca aacaacaccc tctccctcaa gtctttatcg gcggagaccg ggacggctac
     3061 tctcgcgccc gttcaaaaga gcctcttgcc aggagctacg tcgaggtctt ctagctcgta
     3121 gccgaggtcc ctggcgctcg cctcgaagaa ctcccggttg cggtgcgcga acggaagcca
     3181 ctccgtatag cgctcgtagg cttcccgcgc atcgggcagg ctgtcgagga gaaccatctc
     3241 gcacatgtag gaggttatct ggatgaagcg ggagagccat ttgtccgcag gctccttgcc
     3301 acgagaatag gcggcctcga aggtctcgaa ctggacgggc tcggggctct cttcgaggtg
     3361 ctcggagagg aagcgctcgt cgagataaga agttacgttg tcgaggccgg ttatgagagt
     3421 ggggagccag ggagtctggg cgccggggtg gtctttagga agaccccagc ggagggccgc
     3481 cgccatgccg ccgagcatgc ggaaggtgta gggcagggag cggtaggcgc ggcggctgtg
     3541 gtagtagtag acgatagggt agcgccggat gccctcgtag atctcgacca gttcccggtg
     3601 gacggccatc aggtgcgtct ccagaccccg gtgctcgccg ttcgggaagt gcgggatcag
     3661 gatgtccagc ggctcgctcg tgtcctgggc ctgatggtag agaccggcag cgaggatgcc
     3721 gagctgctgc aagaccccgt ataccccgag gacgtaggtg atggcgagcg tcaggatgcc
     3781 gaatcctatg agcgcctccg agacagtcag tagctgatag aaggcgctca gaggcgtcac
     3841 gtcgccgaac cccagggtcg agatagcggt tccgctcaag tagaacgcct ccagcaggga
     3901 tggttcgagg ctggagttcg agaagtagaa agtatgggcg tccatgccgg cgtagtagat
     3961 cagggcgtac ccggacgaga cgagcagtat ccagacgaag atcgtaaccg gcaccatcag
     4021 cggcgccgca agagagagcc cgagagcccg gtacctgccc ggcaacggcc gggtgacgaa
     4081 gctcaccacg tggaacagcc ggttatagag gcggctgctg aggaacccga acccgtcgta
     4141 atggagcacc gtgaagaaca catccgtgag cccggtcagt atcaggatca cgccgacggg
     4201 taagagcaac cactccatga tcggacaata tcacgaacgc gcggacttca ggctcgcaag
     4261 acccaggtgt ccttcgtctt gccgccgctg gagatgttga ctagcttgct gccaggagcg
     4321 gccacccgtg tcatcgcacc gggcacgacc tcggcggtct ccgcctccgg cgcgagggcg
     4381 tggacgcggt agtccgcgtg ggtgtcgcgc aggagcagtt cgtctccttc tgtctcacag
     4441 agcacgtggg tggagaactc cacgacttcc tgggcaacgt actcgaccgg gttctgctcg
     4501 accttctcgc ggaagcgttc gatagtgtct ttcgactctt ccggggcgat cagcacgtcc
     4561 tttcccagcc gccgcggctc tttatgacga ggtccgcgaa gtattacatg acgtattgtc
     4621 tgtcttccgc gacggccagc gagtaggtgc ggacgctctg aaggagaggc tcctggccga
     4681 ggtaggtccg aaccatggcc ggtatgaacg ggcggacgcc ttgtcgtcgg tgatgccgag
     4741 gttgggagcg aagatggcct gtaccctgcg cgcgatctag ccgcaccagg gcgtctcact
     4801 gctcacggac ggaagcatag aggtctgcgt gttccgcgct ccggcccgcg ggacagaagc
     4861 tggcgctcgg atcaggcaaa gaccccgacg agtccgcagg ggaggccgag catgaaggag
     4921 atggagaagg taatggcgaa gaagacgttc tcctcggagg tgtaggagta gactcgtccg
     4981 tagtgcagca tcaggaagac tgagggcacg gagatgccac tcatgagggc ggccgttaca
     5041 cgcagcatgt tgagggagcc tatctgcagg aagactccct gcgagttgag gagcagcagc
     5101 ccgaaccagt ccgcggcgag catgagtgag acgccgaagg ccgcgagagc cgcgtagaag
     5161 agaggccgcg gcaggagcgg gcctttcggc ggggcatcta tctcctcatc ctcttcgaag
     5221 aacgtctttg gcgggcttcg gaagattttc aacttcggga gacggttgga aggcttcatg
     5281 gtccgtgtat agtagcccat cgcgaggctt ttcgtcttgt caggagcgca agaagccgcc
     5341 ttcggaatgt atgacctggc cggtgatcca ggccgcctca tcactcgcta gaaacgtcac
     5401 gagccgagcc gcgtcctcgg gctcccccac cctgccgagc gcgaacttcg gcgcgagatc
     5461 ccgtttcaac tcctccgtca tccagccggt gttggtgggc ccgggattga cggcgttcac
     5521 cgtgatcccc ttctgtccga cctccgcggc tagcgtaacg gtgaacgcct cgatagctcc
     5581 ttttgtcgcc gcgtaggcga gttcgccgac catcggcccg agtgattgtc cggaagtgag
     5641 gttgactatc cttcctcccg gcccacccgg atagcgccgc gcgaactcca cgcacaggag
     5701 cgccatcgcc cgcacgttga cggcgtagtg ggcatccagc atcgccgcat ccaggttctc
     5761 gaagccgtcg cgggtcgagt gggcggcgtt gttgatgagg atggagggcg tcccgagctt
     5821 ttcggtggct gagtcgagga gccgctccgg tgagtcggga cgtgagagat ctacctccac
     5881 accctctgcc cggacgcccg tcgcgcgtag ctcttcgaga agcgtatccg gctcgtcttc
     5941 gtttgaagac cacggcatct cccggtcgta ggccctccag taggagaaga cgatatcggc
     6001 gccgcagagg gccagcgacc ggcagatcgc cgtccctatc cccctccgcc gcccggcgcc
     6061 ggtcactaac gccacgcgtc cgcgcaggag gtcgtccctc gcggttgatc tccgggtttc
     6121 ttcgctcata tcccctccca tgtcggactt tcataaattt gatgcgggcc gctggctccg
     6181 agcggaaggt tatcaggaga aagagcctgc ccggtgatgg tcggccagct tttcgaaggc
     6241 tgttatgcac ctgcggttcg cctggatgcg ccgacggaag ctggaaagcg ttcggtgggc
     6301 ctgtatactc ggctccggca ttatttctta cctctcgaag acaaacagca tactgccgaa
     6361 cggaggtccc atgagcacac agccgaaaag ttacctgctg gacatggacg gagtcctgat
     6421 gcgcggggga gagccgatac cgggcgctcc cgaattcatc gcgcgtctta tagagcgtca
     6481 ggcgaagttc ctcatactga cgaacaactc cctctatacg ccgcgcgacc tgcagctcag
     6541 gctgcggcgc agggggctgg aggtgccggt cgagtctatc tacacctccg cgatggcgac
     6601 cgccgagttc ctggattctc agaccccgaa cggcactgcg ttcgtgatcg gcgaggccgg
     6661 tctgaccacc gcgctgcacg agaccggtta cagcctggtg gataccgacc ccgactacgt
     6721 ggtcctcggc gagacggcca cgttgtcctt ccagcggatc acgcaggcgg tgcggatgat
     6781 ccttgctggg gcgcgcttca tcgccactaa cccggacacg agcggaccga gcgagggcgg
     6841 cattatcccg gcaaccgggg cgattgcggc cctgatctcc agcgccacgg gcgtcatgcc
     6901 gtacttcgtc ggcaagccga acccgctgat ggttcgcacg gcgctcaaca tgatcgacgc
     6961 ccactccgaa gactcggtga tgatcggcga caataaggac acggacgtgg tcatgggcat
     7021 tgagagcggt ctggagacga ttctggtgct aacgggcgta accaccctgg gagaggtgga
     7081 acgccatccc taccgtccga cccggatcgc ggagtcggtc gccgagatta tgccctgagc
     7141 ccgcgctccc cggcttatgt gatccagatc aagccggtgt acaattagcg catgaccgtg
     7201 ttccgctacg gacgggcgta ttacgatgaa ggcgagttcg tgcgccgcaa ggacgcgatg
     7261 ccgggcgacg acctcgaccc tgtggtgcgc gtggtagaga acctggggac gatcgagatc
     7321 ccctgcggca aagtggactg ccggagcaac tgcctggagt acctggtgcg ctcggtccgc
     7381 cgggcggagg actacaacct cgcggagtgt aagctctctc cgctcgtaag cattatctag
     7441 ctttttatcc ctctactcga agtcttcgta ggtcttctgg accggcggac ggtcggcggc
     7501 gtagaggcgc atcagcatcc cccctcccac gggcgagagg aacagccccg tgaggccagc
     7561 cgggagcagg tcgcgggcga tggtccaggc gacgagcagc ggcgtgccgg gcgagaagtt
     7621 ggaggcccgt atgagcttca gggggtccag gactccgccg gcgggaagta tccccgaggc
     7681 gtaggcgacg atggtaagaa gccccgagat gaggcccatc aagagcgcaa gtatcatgct
     7741 gagtgcgaac ctcggtccgt agtaagagga ggactcgtcc gtggctcctc gtctcgccag
     7801 caggattccg ccgaacacga aggcagccac gaagcccgcc acgagccccg ttcccatgta
     7861 gaccagcatc agcgccgcag ggctgccctc gccggtcggc gcgtcggaga aggccaggag
     7921 tattaccggg ctcgggaaga gcgactttat gagggcgaac ccgaagccgt agagcgctcc
     7981 cacgcccatg agacgcaaga acatcacgca tctcctttcc gcacgaggct attgatgagc
     8041 gtcccaaaga ccgggccgag cagcagccca cccgccagga cgtacacgaa gaccgagagg
     8101 gcgacgaaga acccgcccag gaatacccgc gggctcatga gcgtggcgag ctggaccggg
     8161 ttgccgaggg tgaagccgga gagcgttacg gccatcagcg cccagatcag ggctgcggac
     8221 aacgccccgc ccgcaagtcc ctgaaagagc ccggtcccga gagcagccgc gaccgcgccg
     8281 cgcttcttgc cgggacggat ggctcgccgc ccggcgacga gggtcccgac agatccactg
     8341 acgacagagc acgcgacggc cgcgaccagg aaccccgaga ggtgaaacgg ccgcgacatt
     8401 acgagcgcgg gcacggccgc gagaaccacg ccccagaggg cgccgagcag gatcaggcgg
     8461 ttccgaactg cggtagtcat tgtgaagaca ggttatcctg caaggctccg aacgtcagca
     8521 agaggtttca cgaggcggat cttcatggag cgtgtccggc tcggtcgcgg cgctactgta
     8581 cccctcgctc cggtcgccgg gttgatgctg gatgtagtca tatgaatgct ccttctaatt
     8641 cactttcacc ccacgaagat gaaatataca cctcacgtac cgtaatccct ttaggtccct
     8701 ctctaaccca gcgagtacaa tcctcttaca cgcctataca ggagtagaac gtgctgaagg
     8761 tcaagctgga tcactgcgta atccacgtct ctgaatggga gcgctccaac aggttctacg
     8821 aagaagtgat gggcgccgag gtggtcccgg cgggagaacg ctggtcgtac cggttcgggg
     8881 atcagcaact aaacctgcac ggtccgggag ttgatcccac cccggtcgcc aggttgccag
     8941 tacagcccgg caacagcgac ctgtgcttcg agtggccggg acctatcagc gaggcgcgaa
     9001 agcacctgga gcaacacggc gtcgaggtcg agctgggccc ggtcgagcgt aacggggctc
     9061 ggggctccgg cacgagcctc tacttccgcg acccggacgg ttcgctcctg gagttcatct
     9121 cctactccgg cggatagcgt ggcacagcgc cggacagaca cgcacccgga ccccgccgag
     9181 gcgcggcgcg tcctaaagga gcgggcgcgg gaagcgggct tcgacctggt tggcgtgacc
     9241 tccgcggaac cgctgcggga gggcggcgag cgcctgcggg agtggcagga ggcccggatg
     9301 gccgccgaca tgtgctacat gcagcggcct gtcgagttgc tctcggaccc gaagaagctg
     9361 cagaagagcg cccgcagcgt cgtctctctg ggcgtctcgt attacccggg cgatcatccc
     9421 gagaacgcgg acggtggtgg ccgggttgcg cgatacgcgt ggggccggga ctaccacgtc
     9481 gtcatcaagg agcgcctgta ccggctgcgg gaggaactgg agaaagatct cggggtgaag
     9541 gtaaaggcgc gggcgttcac ggacgcggtg ccgctgctgg agcggtcggc ggcgcagcgc
     9601 gcggggctcg ggttcttcgg gaggaactct tgtctcataa acggggatgt cgggtcgtac
     9661 ttctttatag cggacctgac cctggacctg gacctgccga tagacgaaca tggaacgggg
     9721 acgtgcggcc gctgctcccg ctgcatggac cgctgcccga ccggagccat aaaagccccc
     9781 ggcgtggtgg acgcgcggct gtgcatctcc tacctgacca tcgagaaccg gggagagatc
     9841 ccgcgagagc tacgcgagaa ggtcggagac tgggccttcg gctgcgatgt ctgccaggag
     9901 gtctgtccgt ataacaagac gaaggcagca cggagccggt ggccggagtt ctccgaagag
     9961 gccggcgccg gcccgtacct ggacatcgtg gaggtactgg agatacggac ggacgaggag
    10021 ttcgagcagc gcttcgcggg aacgcccctc acccgtcccg gacgggctgg tctcttgcgg
    10081 aactgctgcg tcgcggccgg gaacctcaag caagagcgag cggtgcccgc gctcttgcgg
    10141 tgtcttgcgg aggacgagtc ggagctggtc cggggacacg cggcctgggc gctgggtgag
    10201 atcggcggct cggagaagga gttgcgggag gcactcgcta cggagcggga cgaagggtgc
    10261 cggggggaga tccggcaggc gctgggtgac gggaccggaa gcgcgccggt gggttagaat
    10321 agtcggacga tggcgcgcca gatcaactac tacgagatac tcggcgtctc ccgcgaggcg
    10381 tcccagacgg agatacggaa cgcttaccgc acgctggcga agcagcgcca cccggaccat
    10441 ccgggaggca gcgcggatga cttctccctc ctccaggagg cccacgccac gctctcggac
    10501 ccgaaccgcc gccgccacca cgacgaagcc ctagacctcg ctttcgccgc cgatcaacta
    10561 gccgacctgg acttcgactc cctcggagac gagctctccg ccaagcgccg ggagcgcgag
    10621 tcgagtggcc cgagcttcgg agaacgcctg cggggacgct tcggcagaaa agaggaagac
    10681 ccgttcgagc agctccgcag agagacgagg gagtccggga gaagcagcgg gcgcgaatcc
    10741 gggcggcgcg gccggggccg ctaccgcgag gagcgcgcgg cccgctggta cgagccccac
    10801 gacttcgacc ccgagccgat aggttggaag accggcgcga tcagcttcgt cgccgcattc
    10861 ctggcgttca tcgcggtcgg ccagatcggg ctgtgggcca taaatcccgc gagcgccggc
    10921 tccctggccg gcatctctgt gctggcctcg ttcatgccga tcctctacac gctggtcggc
    10981 cttgtggcct cgtacttcgc ttaccgggca gccgggtacg gcgcggtggg cctggtcttc
    11041 gtggcggcgc tcgtcgtcgg aggacggggc ggcccgaccg ggctcctgca gttcgggacc
    11101 ctgggcatcg tcctcctgct cgtcgtgatc tggctcggca aacgcagaga ccgccccggc
    11161 cgctgaccat tgctatctcg tggagcccta cccacggctc tctgatctct gatgccgaag
    11221 acctcgctcg agtcgtccag aaaaccacaa cgctctcggg agaataaacc atgatctcca
    11281 caacggaaat tcagagcttc gtgtccagct tcgagacgcg ggtcgcaccg gtggaaaaag
    11341 ccttcggcga ggcgtggtgg aacctcgcga caaccgggac cgaagaagcc caggaggagt
    11401 tggtgcgggc cggcaacgca tacgacgcgc tcttcgcgga cgccgaggag ttcgagagga
    11461 tgcgcggctg gtacgaggaa cggggctcgc tgcaggaccc tctgctgcgg aggcaggtcg
    11521 aggtcctgta ccgcgcgttt gccggcaagc agggagaccg ggagatccta cggaatatac
    11581 aggagttgga agccgaggcg aacgccacgt acagcaacca ccggggcgtg gtggacggcc
    11641 gcgagatcgg ggagaacgag gtacgcgagg tcctgcgtag ctcaggcgac gaccgcctgc
    11701 gccgcgaagc ctgggaagcc tccaagacgg tcggccgcga ggtcgaggct acggtgcggg
    11761 agctggcgcg cctccgcaac cgcctcgcgc gggaggctgg atacccggat cactaccacc
    11821 gctcgctgga cttgcaggag ataggcgcgg cggatctggc gcgcctcatg gacgagctgg
    11881 agtccgtcac cgaagccccc ttccgggatc tcaagtcccg cctggacacg agcgtccggg
    11941 aaagattcgg cgtcgagtcc gttatgccct ggcacctacc ggacccgttc tttcaaagtt
    12001 gcaaacagga cccgtctgcg tttccgcgtc cggattcgcg agggggttcg ggaagtgcgt
    12061 ctggagacgg taacggggta gacgtagaca gcttcttccg ggacaaggac ctcgaagcgc
    12121 tgacccgcaa gacttacgac aatatgggcc tggaagtgcg ggacgtaatg gcgaagagcg
    12181 acctttacga gcgggaaggc aagagtcaac acgctttctg tctcggtgtc gggcgggatt
    12241 atccttatga cgtgcgggtt ctggcgaacg tccagcccga ctcctattgg atggacacga
    12301 tgttgcacga gtttgggcat gccgtctacg acaagcacat aaatcccggt ctcccctatt
    12361 tcctgcgctc cattgcacac atcaatacga cagaggccat cgcacttatg atgggatctc
    12421 ttgcggacga tcccgagtgg ctctctgata ttgccggggt ttccgaggcg gaactgagcg
    12481 agaaccggga gtatctacac gggcgcgacc gggctgacaa gctggtcttc acgcgctggg
    12541 cgctggttat gttccatttc gagcaggcgc tctacgccga cccgggccgg gaggatctga
    12601 ataccgtctg gtgggatctc gtggagcgca tacagctagt ggaacgtcca cccgatagag
    12661 atgagccgga ctgggcggct aagattcatg tcgcgaccgc ccccgtctac taccacaact
    12721 acgtcctcgg tcacctgacc gccgcccagc tccggcacta cctggagact tacataactc
    12781 acggtccgtt cttcgagagc gaggtggccg ggcgctacct gctcgaagcc gtcttcggtc
    12841 ccggcgcgcg cgatgactgg cagaccaccg tgctgcgcgc caccggggag aagctgaatc
    12901 cccgatactt cgtggagtcg ctgggctagc cgcttccgcg gaccgggacc tcgacgccct
    12961 cgaatgtccc gtcgcgggcg ctctcggctc cgacccgcag cgtccccctg ccggagaagg
    13021 acggcgcctt cagcgtggtc tcgaagtaac cccaggtaac ggcccagtcg ttcgcctgca
    13081 cggtcttgcg ggcgatcacc tctccaccgg agtcggtcag gacgatggtg ttcgtcccct
    13141 cgaagttgcg ggagtaaccg cttaccgtca gggagcttcc cacacgctcc ccctctctcg
    13201 gctgggtgac gacggtcttg tccccctcag cgggcagcgg catatctagc ggcatctcag
    13261 aaggcttgaa gtccaccacg agccgggccg ggtccgtcag ttccatcacc cggtaagtaa
    13321 acgcctcgtc cgcgacaata tccacgaaca tcccgccatc cgggccgcgc accgcgtgaa
    13381 agtccttgag caacttgccg ccgagcttcc cgtcagatac ctgcgtcgcg ctcacggacg
    13441 gcaggctaac gcgcaataac ccgtctcctg ttgggctcgt gagcttccat tccggtatgc
    13501 tcccagcggg tctagagccg gtcccgagat ccaggacgac gcgctcgtac ccgccgtgat
    13561 ccccatacct cacggccagt atgctgtcgg cctggaaacc ttctcctccg ctcgattccg
    13621 gactggcgat gaagggacct tcgccggaac tttccgcttt cccgccggtc ccggtttgat
    13681 ctgtcgcggt ggtgctcccc ggctcctcct tctccgcgcc gacggtcgct tccgtggagg
    13741 tctccgcggc agtcgtctcg ccagcggtgg tatgggccga ggtcccgccc atagtctccg
    13801 tgccagcgcc atcccggtcc gctccgtctc cccgaccggc gctgccacag cccgcgatga
    13861 agacgagaca gatcagaacc agcggcagga ccattcgtac tctcatgccg ctcctacata
    13921 aaaaggcgta gtcacggggc gcattatagg gacgtgatgt tgcaatggtt ttaagcaggc
    13981 gttgcggttg gtttacaata gccgcgtccc gagcgcgccc ggcgtccggg cgcccgttcc
    14041 gacccgcgag gataagatct tgaagcacag accccggcat gatgagcccc gcgcagcgtt
    14101 tcgcgcgtcg cgcgtaagcg agcatctgtt cgcgctggct ctctacaccg cgcttgctgt
    14161 ggtcttcacc tatccgctcg ccttcaacat ggaccgggtg gacggagctg gagacccggc
    14221 ggtcatggta tggagtatgg cctggatcca gcacgccctc accaccggcg cctccctcta
    14281 cgacgccaac atcttctacc cgacacagaa cgctctcgcc tacaccgacc tcctgctgcc
    14341 gagcgccgtc atcaccaccc cgctctacct ggcgacggat aacccactcc tcagctttaa
    14401 cgtcgtgctc ttactgacct acgtcctctc cggctatgca accttcctgc tcgtccggcg
    14461 gctgctgggc ggcaggccct acgctctgct cgcagcgctc ttcgccgggg ccgtctacgc
    14521 gctctgtcct taccgctacg ggcacatcac ccagctcaac accatgacca cgtactggct
    14581 gcccctgatc ctgctcttca tgcaccgcta cctggaagac ggccgccgct tccgagacat
    14641 gctcctggtc ggcctcttct tcaccctcaa cgtcctctcc ggcctctact acggagtctt
    14701 cgccgtcctg atgatggcct ccttctacct cctctggtcc ttcatgcacc gcgaacctcc
    14761 gaaggttcgg gacttggctc tcggcatccc cgtcttcgcc gctttcgggg cagtgctcgc
    14821 gctcgtcctc ggcccctaca tcgccctgag cggcgcctcc gaccacgcca gagacatcag
    14881 caccgtcgcc ggcggctcgt tcgtcccggc agccatcctg acctccgtcc cggagagctg
    14941 gttcctcggc tggaccccgg aagtcctcgg catcacccac gagaaccgca agcccgtcta
    15001 cgagctcacc ctctaccccg gcctcgccgt taccgcacta gcggtctacg ggctgctgcg
    15061 gaggaaccgc gtcgcgatcc actccgcgct ctacgccctg ctcggcctgg tcatcttcat
    15121 cttctcgtgc ggaccggcta tcccggtggg cgactccttc ataccgctcc cttactacct
    15181 gctctacgag ttcgtccccg gcttcggaaa cctccgggtg cccgcccgca tgtgggccat
    15241 agtcatgctc tgcatcgccg tcctcgcggg gttcggcctt cgcgcgctca tgcagcgcct
    15301 cggcgggaag aaggccctcc tggccctagc tgtaatctcg ctcttcacgg cgttcgagtt
    15361 cgtcccgaag ctacccatag accgctacat agaccggggg ccggcgacta tcgagcccgc
    15421 ctaccggtac ctggcagaca gggcgcccgg cgcggtggtc gccgaagtcc cgttcgccag
    15481 ccccgcggac gccttccgcg agacgccgag gatgtaccgc tcgaccttcg ggtggtggcg
    15541 actcgtgaac ggctacgcga gctacttccc cgacggctat acggagactc gcgacgccct
    15601 gaacaccctg ccaagccccg agagcctgaa ggagctgcgc cgtctcggcg tggagtatgt
    15661 cgtggttcac ccggatcagt acgacgtgga cggcatggac ggcgaggaga tgtcgcggaa
    15721 ggtgggcagg gagccgtcgc tgcagcgcgt cgccggggac ggagagacgg tactcttccg
    15781 ggtgaagcca tgatctcctt gcacgacatc cagacggctc agcggcgtgt acgcggggta
    15841 gccttgcgga cgccgctggt ctcgtgtccg ggcggcgagg aggaccgcgt cctctacttc
    15901 aagcccgaga acctgcaacc gaccggcgcc ttcaagctgc gcggcgccta caacaagatc
    15961 tcctcgctaa ccgaggagga gcgccgcctg ggtgtcgtcg cccactcaag cggcaaccac
    16021 gcccaggccg tggcgtacgc ggcccgcgcc ctgggagtcc gggccgtcat cgtcatgccg
    16081 cgcggcgccc cgaaggttaa gctcgacgcc acgcgggcgc acgaagccga ggtcgtggtc
    16141 gtgggtgacg acagcaccga gcgggcgcac cgggcggagc agttggccga ggagttcggc
    16201 tacactccgg tcccgccata cgacgacgag accctcatag ccggccaggg cacggtggga
    16261 ctggagatcc tggaggacct gcccgacgtg gagaccgtgc tcgtcccggt cagcggcggc
    16321 ggcctcataa gcggagtcgc ggcggcgctc aagctaaacc ggccggatat aaacgcgatc
    16381 ggggtagagc cggagctggc cgccgacgcg cgggccagcc tgcgcagcgg ccggat
//
LOCUS       DASSMB010000036         7287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1655299, whole genome shotgun sequence.
ACCESSION   DASSMB010000036 DASSMB010000000
VERSION     DASSMB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655299"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VFJ72_02590"
     CDS             complement(<1..182)
                     /locus_tag="VFJ72_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478385.1"
                     /translation="MDFNTGGGTGGSASPGGSSGGTGGAPPRMTGGTGGEFDYRDPVQ
                     SFIRTVTSVVMRPVPF"
     gene            320..1375
                     /locus_tag="VFJ72_02595"
     CDS             320..1375
                     /locus_tag="VFJ72_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478386.1"
                     /translation="MSSTIETLERELGEAQVEREKEERAVVRAENAHDAACAARDDAW
                     SESFATDPEVERLTALVYLRRRELYEAIDRLKAAEGRERRIKSRLMYVLPAERRRYLS
                     GRVDEEFEALLTAIREAYATAISRVEVGVVTERPGDMDLRGDATWRALERLYSGTVRR
                     WVEVRAESYHAEVWRMAAQISYYAGGHTPRVFVGGEEARAGVLRYQPASWPPLAGYGG
                     EEGRLALARITARRYLDETAIGRLEKRAPALVQVAEIARLYQTMSLTGDEIRWRLEQL
                     VSLRLEVLEAVHRLTGERRRFSFYEIARAARLLDGHFPDPGVAAEALVRSVERALETG
                     VPLVDVAYLSVTTGDGF"
     gene            complement(1383..1517)
                     /locus_tag="VFJ72_02600"
     CDS             complement(1383..1517)
                     /locus_tag="VFJ72_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478387.1"
                     /translation="MEILEVRRGEFVPDFVYKVRVLAERPARPYNLSVPEHDLSDEPD
                     "
     gene            1690..2919
                     /locus_tag="VFJ72_02605"
     CDS             1690..2919
                     /locus_tag="VFJ72_02605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7478388.1"
                     /translation="MTERYDLIVVGAGPAGSATAYYAARAGLNTLLLDRQEFPRDKPC
                     GDGLMPHAASEVTLMGLGDWLDEPHHGKFTDISIYTQTAFLRQRVPPTLHGPRGYIAA
                     REETDAKLLERAVGAGADFRARHRATKLRRNPAGEATGIEAENASGALRFDAPLVVAA
                     DGIGGFASEGMKAHQNAVARRQYFKNVTGPNHQDIHVFITKDMNEDGAGYGWVFYFGD
                     GRANVGAGVSTRALARSGRNLKDYFDRFLEEPVLAEWLEGAEAEAPPKSWSLKMGMWG
                     AKRYSQGLIMVGDAGSMIHPISGEGVGYALESGRLAASWAHEAHSRRDFSAATLAGYE
                     RQLRHKRAREHYSGHAMVNLVPNLALMEPLFRACEKDPEARRTLVEGFTGDAPIYSLL
                     KHPKTLAAAVREGIKGG"
     gene            complement(2936..4021)
                     /locus_tag="VFJ72_02610"
     CDS             complement(2936..4021)
                     /locus_tag="VFJ72_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7478389.1"
                     /translation="MGDARVGYRWLFLAVVAFMLAGTLLYGRSAVAASPYDSEELQFL
                     SLINEYRQNNGLQPMILSDTLTLTSGHHDEDMAKYGFFAHDTVRSSYYPAGSKPWDRM
                     AKDGYGYNTYRGENLAVGYETAEEAFQAWRNSPAHNAAMLDGHYKVIGIARLNAPGSE
                     WGWYWTTDFGGVVDPSAHGANEAPKPKDPAPEPTGTQAASPEPKKPDPPAPAKKSAPS
                     RDQGGIENGSFEDGTVWRQDAKDGADLILPGGVARLGDYADGRDDLSQVIKVTRGAKV
                     SYRIKVISEERKDPSDHLKVRLTDTKGKKLAVLKVHNASDPAGWQRVTLDVSRYSGRT
                     VRLDFSAVMDDQRLTTFLLDDVALKAR"
     gene            4235..5035
                     /locus_tag="VFJ72_02615"
     CDS             4235..5035
                     /locus_tag="VFJ72_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7478390.1"
                     /translation="MATRTTFEAGTTRLHVLDDGIFITDAGNIFGGSRKARIRGAMHA
                     VLVEAGDDLVLLDAGFGPELPEGLVGRYELKREKSLMDGLREAGHDPEDVTRVVLSHL
                     DADHVGWALAPPSFPNATVYVQQAALEEARGKPETDGRRMAVPAVEQGVEEGWCELLE
                     GDGEVVPGIRVQVRTGHSAGHQIVWIGSGKNAALFTADLAPSKIFLNPDTIAGVDTDP
                     EAARRNRIEVLSEAEEKNAPVILYHEPKDFLVNIRRSEKGFEGVPLEG"
     gene            complement(5187..5762)
                     /locus_tag="VFJ72_02620"
     CDS             complement(5187..5762)
                     /locus_tag="VFJ72_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HET7478391.1"
                     /translation="MELTEAIITRQSINRVKQDPVPRALVERILESAIHAPNHKITEP
                     WRFHVFTGKGRGELARARAELARIQAEEEGEDEEMAAGRISRERKKTFRAPVVIAVIS
                     LAGRDEVETLENYAACCAALQNMQLTAHSLGLASIWRTGPVAYHEHMRGFFGLADGDR
                     IVAYLYIGYPDMGERPRRRAPAAEKTVWHEG"
     gene            5925..6899
                     /locus_tag="VFJ72_02625"
     CDS             5925..6899
                     /locus_tag="VFJ72_02625"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7478392.1"
                     /translation="MSKTVTVTGAAGAIGYAILFRIASGQMLGPDEKIRLKLLEIEPA
                     LKAAEGTAMELYDCAFPLLESIDITADANEAFDGTNVGLLIGARPRQKGMERADLLEA
                     NGAIFKPQGEAINANAADDVKILVVGNPANTNALIAMNNAPDVPPERFTAMTRLDENR
                     AVSMLAQKLGVGVEDVQDLVVWGNHSPSMFPDLFNAKVHGERAVDQVDMDWYENEYMP
                     KVGKRGAEVLEARGASSAASAANAAIDHVRDWSLGADGLRSMAVRSGGQYGVDEGLLS
                     SFPVRCSGGDYEIVEGLEIGEFAQSKLDITVGELKEERDAVRKLGLIG"
     gene            6977..>7287
                     /locus_tag="VFJ72_02630"
     CDS             6977..>7287
                     /locus_tag="VFJ72_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET7478393.1"
                     /translation="MTPEATPGFKKRLREALDEMHVGQTFSYTRTFTDGDVALFCGVT
                     DDYNPYHQDESFARESWYGQRTIPGLLTGSMLTHIGGMLGFLATEMSFEYVAPVYAGE
                     T"
BASE COUNT         1275 a         2337 c         2460 g         1215 t
ORIGIN      
        1 aagaatggga ccggtcgcat gaccaccgac gtgaccgtcc tgataaagct ctgtaccggg
       61 tcgcggtagt cgaactcgcc acccgtgccc ccggtcattc ttggcggcgc gccgcccgtt
      121 ccgccggacg aacctcccgg actcgcgctg ccccccgtgc cgccacccgt gttgaagtcc
      181 acgcggctcc cctttccatt cgcgtcgtgg tctactgaaa tctacctgct cgggcggacg
      241 aacgcaagaa aggtccgtcg agcccggctt gctgtagaat ggtacagacg tacttctcag
      301 gggcaatggg gatcttctga tgtcgtccac tatcgagaca cttgaacgtg aactgggcga
      361 ggcccaggta gagcgagaga aggaggagcg ggccgtcgtg cgggccgaga acgcccacga
      421 cgccgcctgc gccgcccggg acgacgcctg gtccgagagc ttcgccaccg acccggaggt
      481 agagcgtttg accgctctcg tgtacctgcg gcggcgggag ctctacgagg ccatagaccg
      541 cctcaaggcg gccgaggggc gggagcgaag gatcaagagc cgtctgatgt acgtcctgcc
      601 cgccgaacgc cgccgctacc tctccgggcg cgtggacgag gagttcgagg cgctgctaac
      661 ggcgatccgg gaggcgtatg ctacagcgat ttcgcgggta gaggtcggcg tcgttacgga
      721 gcggccggga gacatggacc tgaggggaga cgcgacgtgg cgcgccctgg agcggctgta
      781 ctccggcacg gtgcgacggt gggtggaggt ccgggcggag tcctaccacg cggaggtatg
      841 gcggatggcg gctcagatct cctactacgc cggaggccat acgccgcgcg ttttcgtcgg
      901 gggcgaggag gcgcgggccg gcgtcctgcg ctaccagcca gcctcctggc ccccgctcgc
      961 cggatacggc ggcgaggagg gacgtctggc gctcgcgagg atcacggccc gccgctacct
     1021 ggacgagacg gccatcggcc ggctggagaa gcgcgctccg gccctggtgc aggtcgcgga
     1081 gatcgcacgc ctgtaccaga ccatgagcct gacgggagat gagatccggt ggcgcctcga
     1141 acagctcgtc tcgctccgcc tcgaagtcct ggaggcggtc catcgtctta cgggcgagcg
     1201 ccgccggttc tccttctatg agatcgcccg cgccgcccgc ctgctggacg ggcatttccc
     1261 ggatcccggc gtcgccgccg aggccctggt ccggtccgtc gagcgcgccc tggagaccgg
     1321 tgtccccctg gttgacgtcg cctacctgag cgttaccacg ggcgacggct tctaggccgt
     1381 tctcaatccg gctcgtcgga gaggtcgtgc tccggcaccg acaggttata cggtcgggcg
     1441 ggccgctccg cgagcacgcg caccttgtag acgaagtccg ggacgaactc cccgcgccgg
     1501 acctccagaa tctccaccgg ctggcccgtc gccttgagcg cggcgcggcc tccgacctcg
     1561 tagcgggcgg ggcggttgcg gtcgactatc gtgggtctcc tctccgttaa cgtgccacgt
     1621 ataatgatct catcccctcc ataacggcgg ggcgacggat attctgacac agcggcgaga
     1681 ggaagaacga tgacggaacg ctacgatctc atagtcgtgg gcgccggacc ggccggctcg
     1741 gcgaccgcct actacgccgc ccgcgccggc ctgaacaccc ttctcctgga ccgccaggag
     1801 ttcccgagag acaagccctg cggcgacggc ctgatgcccc acgccgcctc cgaggtcacg
     1861 ctcatgggtc tcggcgactg gctcgacgaa ccgcaccacg ggaagttcac cgacatctcg
     1921 atctacaccc agaccgcctt cctgcgccag cgcgtccccc ccaccctcca cggcccgcga
     1981 ggctacatcg ccgcccgcga ggagacggac gcgaagctcc tcgaacgcgc cgtcggagcg
     2041 ggcgccgact tccgggcccg ccaccgggcc acgaagctcc ggcgtaaccc ggcgggagag
     2101 gcaaccggca tagaggcgga gaacgcctcc ggcgccctgc gcttcgacgc cccgctggtc
     2161 gtcgccgccg acggaatcgg cgggttcgct tcggagggga tgaaggccca ccagaacgcc
     2221 gtcgcccgaa gacagtactt caagaacgtc accggcccga accaccagga catccacgtc
     2281 ttcatcacga aggacatgaa cgaggacggg gccggatacg ggtgggtctt ctacttcggc
     2341 gacgggcggg ccaacgtggg agcgggggtc tccacgcggg ctctggcccg cagcggacgc
     2401 aacctcaagg actacttcga ccgtttcctg gaggagcctg tgctcgccga atggctggag
     2461 ggagcggagg ccgaggcgcc gccgaagagc tggtcgctca agatggggat gtggggagcg
     2521 aagcgttact cgcagggcct catcatggtc ggagacgcgg ggagcatgat ccacccgatc
     2581 agcggcgagg gtgtcgggta cgcgctggaa tcgggacgtc tggcggcgtc ctgggcgcac
     2641 gaggctcact cccgccgcga tttctccgct gcgaccctgg cgggctacga gagacaactg
     2701 cgtcacaagc gcgcccgcga acactactcc gggcatgcga tggtcaacct cgtcccgaac
     2761 ctcgcgctca tggagccact attcagggcc tgcgagaaag acccggaggc tcgccggacg
     2821 ctcgttgagg gcttcaccgg cgacgcgccg atctacagcc tgctaaagca ccccaagacg
     2881 ctggctgcgg cggtgcgcga ggggataaag ggaggctaga ggctcctggc cggtactacc
     2941 gcgccttcag cgccacgtcg tcgaggagga aggtcgtcag gcgctggtcg tccatgacgg
     3001 ccgagaagtc caatctcacc gtgcgtcccg agtagcggga gacgtccagc gtcacacgct
     3061 gccagcccgc ggggtcggag gcgttgtgga ccttgagcac ggccagcttc ttccccttcg
     3121 tgtcggtcag gcgcaccttg aggtggtcgg aggggtcctt gcgctcctcc gagatcacct
     3181 tgatcctgta cgagactttg gcgccgcggg ttacttttat aacctggctc aagtcgtcgc
     3241 ggccgtcggc gtagtcgccg agacgggcca cgccgccggg caggataagg tcggcaccgt
     3301 cctttgcatc ctgccgccat acagtcccgt cctcgaacga tccgttctcg atgccgccct
     3361 ggtcccgcga cggggcggac ttcttcgccg gcgctggcgg gtccggcttt ttcggctccg
     3421 gtgacgcggc ctgtgtcccg gttggctcgg gggcagggtc tttgggcttt ggggcttcgt
     3481 tggcgccgtg ggcggagggg tccacgacgc ctccgaagtc ggtggtccag taccagcccc
     3541 actccgagcc gggggcgttg aggcgggcta tcccgatgac cttgtagtgc ccgtcgagca
     3601 tcgccgcgtt gtgggccgga gagttgcgcc aggcctggaa ggcttcctct gcggtctcgt
     3661 acccgacggc gaggttctct ccccggtagg tgttgtagcc gtagccgtcc tttgccatcc
     3721 ggtcccaggg cttggagccc gccgggtaat aggagctgcg cacggtgtcg tgcgcgaaga
     3781 agccatactt cgccatatct tcgtcatggt ggccggaggt aagggtgagg gtatcggaga
     3841 ggatcatggg ctggaggccg ttgttctgcc ggtattcgtt gatgaggctc aggaactgca
     3901 actcctcgga gtcataggga ctcgcggcga cggcgctcct gccgtagagc agcgtcccgg
     3961 ccagcatgaa cgcgacgacc gcgagaaaga gccacctgta acccaccctc gcgtctccca
     4021 tctctatctc cctccgcgtc tgcatgagag ggattatgca ccatgcgccg ggagcccgaa
     4081 tcggtcaaac aggcgaactt ttactagccc aaactggcta gggatgctac cggacagggc
     4141 gcgctggcgc gagacggaat tcggatgatg ccgggagcat gaggcgcgat ggcccgctat
     4201 gatgcgatga acggcgacag gaggcttgcg cgatttggct accaggacaa ccttcgaggc
     4261 aggaactacg cggctacacg ttctcgatga cggcatcttc atcacggacg cgggcaacat
     4321 tttcgggggg tcgaggaagg cccgtatccg gggagccatg cacgcggtgc tcgtcgaggc
     4381 cggagatgac cttgtgctgc tcgacgcggg gttcggcccg gagttgcccg aaggtctcgt
     4441 cggacgctac gagttgaagc gcgagaagag cctcatggac ggcctccgcg aggcggggca
     4501 tgatcccgag gacgttacgc gcgtcgtact ctcgcacctc gacgccgatc acgtcggctg
     4561 ggccctggcg cctcccagct tccccaacgc gaccgtctac gtgcaacagg ccgcgctgga
     4621 agaggcgcga ggcaagcccg agacggacgg acgccgcatg gcggtcccgg cggtcgagca
     4681 gggcgtcgaa gagggctggt gcgagctgct ggagggagat ggcgaggtgg tgccggggat
     4741 aagagtccaa gtgcgtacgg gacattccgc gggacatcag atcgtctgga tcggctcagg
     4801 taaaaacgcc gcgctcttca cggcggatct ggcgccgagc aagatctttt tgaacccgga
     4861 taccatagcc ggcgtggaca cggaccccga agcggcccgg cgtaaccgga tcgaggtgtt
     4921 gtccgaggcg gaggagaaga acgcgcccgt tatcctctac cacgaaccga aagacttcct
     4981 cgtcaacatc cgccgctccg agaaaggctt cgagggagtg ccgctggagg ggtaggctaa
     5041 cgtcctttac gacgctgccg gatcttgcgc cacacaatgg gcgcggcgag tatgagcagc
     5101 cttctcagca aagtcttcac gcaatctcct cgtctaggtc aacagtctga tacgtcgata
     5161 ccctcaggga agttgcgcca acccacttat ccttcgtgcc agacggtctt ctccgctgct
     5221 ggagcacgcc tgcgcggcct ctcccccata tccgggtatc cgatatagag gtaggcgacg
     5281 atcctgtcgc cgtccgccag accgaagaac cctcgcatat gctcgtggta ggcgacggga
     5341 ccagtgcgcc agatgctcgc aagtccgagc gagtgcgctg ttagctgcat gttttgcagg
     5401 gcggcgcagc aggcggcgta gttctcaagc gtctccacct cgtcgcggcc ggcaagggag
     5461 atcaccgcga tcaccaccgg cgcccggaag gtcttcttgc gctcgcggct gatcctgccg
     5521 gccgccatct cctcgtcttc cccttcttct tcggcctgga tgcgagccag ttcggcgcgg
     5581 gcgcgggcca gttctccgcg ccccttaccg gtaaagacat ggaagcgcca gggctcggtg
     5641 atcttgtggt tcggcgcgtg gatggcgctc tccagaatcc gctccaccag cgcgcggggt
     5701 acgggatcct gtttcacccg gttgatgctc tggcgggtaa ttatggcctc ggttaactcc
     5761 aaagatgctc cctgcccgtc tcgaaatgtc ctagagtatt ttactgcgga gtgtagcggc
     5821 agagaaatgg gagttcctct atcattatca gggagacgac cgggaagttc ctcgcgtccg
     5881 tcgacgagaa actccagaaa acgatgggat aaggagatca cctgatgtct aagaccgtca
     5941 ccgtcaccgg cgccgcgggc gcgatcggct acgcgatcct gttccgcatc gcctcgggcc
     6001 agatgcttgg cccggatgag aagatccggc tgaagctcct ggagatcgaa cccgccctga
     6061 aggccgcaga gggaaccgcg atggagctct acgactgcgc cttcccgctg ctggagtcca
     6121 tcgacatcac cgccgacgcg aacgaggcgt tcgacggaac caacgtcggc ctcctgatcg
     6181 gcgcccggcc gcgccagaag gggatggagc gcgccgacct gctggaggcg aacggcgcga
     6241 tcttcaagcc gcaaggcgag gcgattaacg ccaacgcggc agatgacgta aagatcctgg
     6301 tcgtcggcaa cccggcgaac acgaacgccc tgatcgcgat gaacaacgct cccgacgtgc
     6361 cgccagagcg cttcaccgcc atgacgcgac tagacgagaa ccgcgccgtc tcgatgctcg
     6421 cccagaagct cggagtgggc gtcgaggatg tgcaggacct cgtcgtgtgg ggcaaccact
     6481 ccccctcgat gttccccgac ctcttcaacg cgaaggtgca tggcgagcgg gcggtagatc
     6541 aggtagacat ggactggtac gagaacgagt acatgccgaa ggtcggcaag cgcggcgcgg
     6601 aggtcctcga agcgcgcggc gcgtcctcgg cggcctccgc ggcgaacgcg gccatagatc
     6661 acgtccgcga ctggtcgctg ggcgccgatg gtctgcgctc gatggcagtc cggtcgggcg
     6721 gccagtatgg tgtggatgag ggcctcttgt cctcgttccc ggtgcggtgc tctggcggcg
     6781 attacgagat agtggagggg ctggagatcg gcgagttcgc gcagtccaag ctcgacatta
     6841 ctgtgggcga gctaaaggag gagcgcgacg cggtaaggaa actcggcctg atcggttaga
     6901 aggccgccct ggtccggcac cggggagagg aggtattccg ggggccggcc catcgagccg
     6961 aaagaggagg cacggattga cgccggaggc gacgcccggg ttcaaaaaac ggctgaggga
     7021 agcgctggac gaaatgcatg tcggccagac attctcctac acgcgcacct tcaccgacgg
     7081 ggacgtggcg ctgttctgcg gcgtcacaga cgactacaac ccctaccacc aggacgagtc
     7141 cttcgcccgc gagagctggt acgggcaaag gaccataccc ggcctcctga ccggcagcat
     7201 gctcactcac atcggtggga tgctgggttt cctggcgacc gagatgtcct tcgagtacgt
     7261 agcccccgtg tacgccggag aaacgat
//
LOCUS       DASSMB010000037         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_8840, whole genome shotgun sequence.
ACCESSION   DASSMB010000037 DASSMB010000000
VERSION     DASSMB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8840"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VFJ72_02635"
     CDS             complement(<1..588)
                     /locus_tag="VFJ72_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET7478394.1"
                     /translation="MILNPTANKGRSARSREKISRFLNARGMDAVWHVTKGPGDATRI
                     AANLPDGSLAVAVGGDGTVSEVAAACAGTSRVLGVLPLGSGNDYVKVLGIGTKLEDAL
                     EALARGKVRSVDAGEANGVRFNNGLGIGFDAQVAAGVAEAPALLGGFGRYMWSVGRLL
                     WGFRCHDAVLRLDGERVVEARTILIAAALGTTYGAR"
     gene            918..1502
                     /locus_tag="VFJ72_02640"
     CDS             918..1502
                     /locus_tag="VFJ72_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478395.1"
                     /translation="MRVALEREGSTIRAYMVREIENCLHLQGLRGTVGLEYRCPACEA
                     HLDMDGVARWVREAERARDEAYSMIQGDKFGEIAREESQREVYRRRKVMYELQNIPQA
                     VAARPEMILVIYDAAGDSYGCKIFYKEPRPVAGLEEIEIEAGWNAILELRSHPDPVVR
                     IAAEKVEEFHGQRQAASRGEMLPPTRRVFYASEL"
     gene            complement(1699..2256)
                     /gene="thpR"
                     /locus_tag="VFJ72_02645"
     CDS             complement(1699..2256)
                     /gene="thpR"
                     /locus_tag="VFJ72_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7478396.1"
                     /translation="MAVRSFVAIFPPRGIQEALHRSARGLPIEGAVRQVRPENIHLTI
                     KFLGDVQPESLPHIGEALAAVCERHGPFHIDLSGFGAFPSERRGRVLWAGVDGGSARL
                     ISLSEDLEKTLEPLDFEREPRAFRPHLTLGRARGRSFVLGEAATAERLAFRAGRLTLV
                     QSVLREDGARYETVGDYPLGGTKPG"
BASE COUNT          423 a          731 c          732 g          417 t
ORIGIN      
        1 gcgcgccccg taggtcgtcc cgagcgccgc cgcgatgagt atggtccttg cctcaacgac
       61 ccgttcccca tccagccgca ggaccgcgtc gtggcaccgg aatccccaga gcagccgacc
      121 gaccgaccac atgtaccggc cgaaccctcc gaggagagcc ggcgcctccg ccaccccggc
      181 ggctacctgc gcgtcgaagc cgatgccgag gccgttgttg aacctgacgc cgttcgcctc
      241 gcccgcgtcg acagaacgga ccttccctct cgccagcgcc tccagagcgt cttccagctt
      301 cgtaccgatg ccgagcacct tgacgtagtc gttgccgcta ccgagcggca ggaccccgag
      361 gacccggctc gtacccgcgc acgccgcggc tacctcgctt accgtaccat ctcctccgac
      421 cgctactgcg agcgagccgt ccggcaggtt cgccgcgatg cgagtggcgt ctcccggccc
      481 tttcgttacg tgccagaccg cgtccattcc acgagcgttc aggaagcggg agatcttctc
      541 tctgctcctc gcggaacgcc ccttattggc cgtcgggttc aagatcaaat gcgttatctg
      601 cccccgaatc gtcatgcccc ggagaatacc agcaccaatc ctcagccgcc tgtcggaccg
      661 taaaggatca tgctgcactt caggtatagg gcgttgctcg ggctccgagt aactcacccg
      721 cgcacgagac ctcgcaatgt acggcgcccc ctcgtctacg aagcaggaaa ccgcggcgcg
      781 gccaggaatg gcgcatttca acgccaaacc agcggcgatt cggttccggc aggccaaacc
      841 gggggcgctg acgaaacctt cgactttgcc gtatacatag acctcacgtt caagtacact
      901 gtatcttgag gtgcgggatg agagtcgcgc tggagcggga agggagtaca atacgggcct
      961 atatggtgag ggagatcgaa aactgcctgc atctccaggg cttgagggga actgtggggt
     1021 tggagtatcg gtgtccggcg tgcgaggcgc atctggatat ggatggagtg gcgcggtggg
     1081 tccgggaggc ggagagggcg cgagatgagg cttactctat gattcaaggt gataaattcg
     1141 gggagatcgc gcgtgaggag agtcagcggg aagtctaccg gcggcggaag gtgatgtacg
     1201 agttgcagaa cataccgcag gcggtagcgg cccggccgga gatgatattg gtgatctatg
     1261 acgctgccgg agactcttac gggtgcaaaa tcttctacaa ggaaccgcgt ccggtcgcgg
     1321 ggctggagga gatcgagata gaggccgggt ggaatgccat tctggagttg cgctcccatc
     1381 cggaccctgt ggtgaggatc gccgccgaga aggtggagga gtttcatgga cagcggcagg
     1441 cggcctcacg gggcgagatg ctccctccga cccgtcgcgt cttctatgcc agcgaactct
     1501 agttacagtg gcctagccgt cattggcttt gccactggcg agccgggcgc atagcggcta
     1561 gaatccgggg tgtgagagtg tgcagtagaa ccggctggaa aggcgcggcc cgatgagcgg
     1621 gaacaacttc atagcgatcg tcgtgatgat cctgatccta ctcttcatcc tcatggccgt
     1681 cgggcctctg gtctcgatct agcccggctt cgtcccaccc aggggatagt ccccgacagt
     1741 ctcatacctc gccccgtcct cccgcaacac gctctgaact agagttaggc gtccggcccg
     1801 gaatgccagt ctctccgccg tggcggcttc tcccagaaca aatgatctgc cgcgcgcacg
     1861 tccgagtgtc aggtgcggcc ggaaagcgcg aggctcgcgc tcgaagtcca gtggctccag
     1921 ggttttctcc aggtcttcgg agagcgagat caggcgtgcg cttcctccgt ctacgcccgc
     1981 ccatagaacg cgccccctgc gctccgaggg gaaggctccg aacccggaca aatcgatgtg
     2041 gaacggaccg tgccgttcgc ataccgcggc cagagcttct cctatatggg gcaggctctc
     2101 cggctgaaca tcgcccagga acttgatcgt caggtggata ttctcgggac gcacctgccg
     2161 gacggctccc tcgattggaa gtccgcgcgc cgagcggtgc agcgcttcct gtatcccacg
     2221 cggtgggaag atggcgacga aggagcgcac ggccatcgca ggttcctatc cctggccgga
     2281 ggcgccgttc tccagcgggc cgg
//
LOCUS       DASSMB010000038        39596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_896685, whole genome shotgun sequence.
ACCESSION   DASSMB010000038 DASSMB010000000
VERSION     DASSMB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 39596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39596
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896685"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2253
                     /locus_tag="VFJ72_02650"
     CDS             <1..2253
                     /locus_tag="VFJ72_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET7478397.1"
                     /translation="YDVDTWQYAGMEVSGSAGKREQGGVTGEQAHYAQGGQLEHGEPT
                     EMDESLEHPRCVFQLLKKHYRRYTPEAVERICGCPKEKFLEVAETLCENSGRERTSAF
                     CYAVSWTQHTVGVQNIRAATIVQLLLGNIGRPGGGIMALRGHASIQGSTDIPTLYNIL
                     PGYIPMPHAHAHAHQDLDEFIEKNAAKTGYWGNMRAYMVSLLKAYFGDAATEENDYCF
                     DHLPRLTGDHSIYPAIMGMMDGWCKGFFVMGENPAVGAANGKLQRLALANLDWLVVRD
                     FSEIETASFWYDSPEIETGELKTGEIGTEVFFMPAAAHTEKDGSFTNTQRLLQWHHKA
                     VEPKGDCRSELWFMYHLGRKVREKLANSTDPKDRAVLDLAWDYPTLGTHEEPDAETVL
                     REIGGWDENGDALESYLPLKDDGTTTCGCWIYAGCFADEVNQTARRKPGSEQSWVAPE
                     WAWAWPMNRRIIYNRASADPEGRPWSERKKYIWWDEEQEKWTGEDVPDISPTKRPDYE
                     PPDDATAEDAIRGDSPFVMQADGRGWIFVPAGLTDGPLPTHYEPHESPFENALYGQQS
                     NPARQQFPRGDNPYNPSDGQSGTRVFPYILSTYRLTEHHTAGGMTRTVAHLSELQPEM
                     FCEVSPELAQERGLENGGWATIVTTRSAIEARVLVTDRLAPLRVQGRTVHQVGMPYHW
                     GSKGLVTGDSANDLLGLVLDPNVHIGEYKVATCDIRPGRRPRGPALARLVDDYRARAN
                     AHAENGGARA"
     gene            2250..3170
                     /locus_tag="VFJ72_02655"
     CDS             2250..3170
                     /locus_tag="VFJ72_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7478398.1"
                     /translation="MSETNVIPGSGSPDAFDIQGRETLETRPVIPLGQKGYEDGQPRM
                     GFFTDTTVCIGCKACEVACKEWNDVPALPFELTGDSYDNTGALGADSWRHVAFIEQRK
                     PVKVVQDAGLTTPASGDTDFRWLMSSDVCKHCTDAPCLDVCPTGSLFRTEFGTVVVQE
                     DICNGCGYCVPSCPFGVLDQRQDDGRVWKCTLCYDRLKGGMEPACAQACPTDSIQFGP
                     LEELREKANYRLEMVHEAGVTDARLYGADEEEYGMGAFFLLLDEPEVYGLPPDPEVVT
                     RKLKSLWGPAAVAAGAMAVGVVAAFAGEGR"
     gene            3167..4126
                     /gene="nrfD"
                     /locus_tag="VFJ72_02660"
     CDS             3167..4126
                     /gene="nrfD"
                     /locus_tag="VFJ72_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7478399.1"
                     /translation="MNARPEVSVSEEARHDGRGTGAEARSYYGRPVLKEPVWSREIPW
                     YFFSGGLAGASASLAAAARLAGNVELARRARLVVLAGAAVSPVLLISDLGKPERFYNM
                     LRVIKPTSPMSVGTWILSAFGTSVGIAAASKVLGVFPRLGRLSGTAAALLGPALSTYT
                     AVLIADTSIPVWHEARREMPFIFAASSAASAGAAAAILTPVEAARPARRLAVGGALAE
                     IAGVEVMKRRLGKLLAEPYEKGEAGRYDRLATAATGLGAAVMGMFGRRHRSAVVAGGA
                     LVLAGAALERWSIFKAGFQSARDPRYTVIPQRERRESRARADG"
     gene            4228..4812
                     /locus_tag="VFJ72_02665"
     CDS             4228..4812
                     /locus_tag="VFJ72_02665"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HET7478400.1"
                     /translation="MAAALVGFLAVAGVLSEASTSTQVGGAAADAAESLPAAETTSVT
                     VDKSATAGKLKWTIHDASRMAEIRKFTIPSTTKRGDFVRVTFTVKNVSKTPVTLDARS
                     LTLIDKTGFKDYPAAGVNSEYIVPSKAILFNDEGLLDPGEERNGEVNFDLAVPFGAHP
                     SGLRLGDTGTSGFHLELGDAEPTAHHEKTVKLGF"
     gene            4861..5940
                     /locus_tag="VFJ72_02670"
     CDS             4861..5940
                     /locus_tag="VFJ72_02670"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478401.1"
                     /translation="MFDSLSLPLLILVFAGAAGAVWVAGVGLSDTTDVLSQRFGLGEA
                     LGGLIMLAIVTNLPEIAITASAALSNNLGVAVGNILGGIAIQTVVLAALDVFGVRGRE
                     PLTYRAASLMLVLEGALVVAVLTVAVMGSQLPSSLIFWRLEPGGLLIAALWVVGLLLL
                     NRARSGLPWQEKGEAPEGQEKPRGHSKAHKDRQAHSKGVGTGRAAIIFAVAAVVTLVG
                     GVVLEQSGENIAGNIGMSGVLFGSTFLAAATALPEISTGLTSVRLGDFKLAVSDIFGG
                     NAFLPVLFLMASLLSGQAVLPQAHDTDIYLTGLGILLTAVYIWGLVFRPRRRVLRMGV
                     DSLAVLLLYVVGIAGLVAVALGGTG"
     gene            6133..6411
                     /locus_tag="VFJ72_02675"
     CDS             6133..6411
                     /locus_tag="VFJ72_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478402.1"
                     /translation="MFFGGAIVAFAMVAVLAFGGVASEVGGSNDIKAWGSFRFLLVGL
                     AVGSAWLESAYVPSLPGWPVGAFLATAAYLTITGAENSAAGTGESRES"
     gene            complement(6437..6565)
                     /locus_tag="VFJ72_02680"
     CDS             complement(6437..6565)
                     /locus_tag="VFJ72_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478403.1"
                     /translation="MMLELSLLPPLVRFFLGFFLILLTTGVAVLILHWLVKGEEEE"
     gene            complement(6562..7923)
                     /locus_tag="VFJ72_02685"
     CDS             complement(6562..7923)
                     /locus_tag="VFJ72_02685"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7478404.1"
                     /translation="MPIGDLQIPLIGKDLVIPLLVMPHILIAAFVIGINLIGAISEWI
                     GSMTKRPYYDRFARNAAKFTLLLFGVGSAIAFTFILSLITLYPVFWSFMQNVFFWVLL
                     AEAFMFVGEVIMVYAWYHSWDTMAYRKNLHVTFGLIAGLFGLTQMIFINVVASYMLTP
                     ANAQASNVGWTFMNPTYMALNMHRFVGNFSYAGFLIAGWGAWRYLRSTTEEDREYYDW
                     MGHWGIIWGFGFLLLQPFMGYEYMTEIKSASPDAFKYLMLGDKSWLFNMLVTELLIMG
                     AASVAYFVHKLKFAVKPVPGLRKLTMGALGFVTLFGLLNMIPADLYLVPQIGLVFGER
                     VNPNIALFDKPPIPLGAMYPYKYIGLIGLAVVGLFALALYLRASATGFYWGRASRWSQ
                     FALLLCAVTVVFTMMTMGYARETARRAGNGSDGWLVHNCITLKDQKFVQKGCPSAAPR
                     ETP"
     gene            complement(7936..8433)
                     /locus_tag="VFJ72_02690"
     CDS             complement(7936..8433)
                     /locus_tag="VFJ72_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochromesubunit B of the bc complex-like
                     protein"
                     /protein_id="HET7478405.1"
                     /translation="MSEIRHGPPKAVEEGEEITEENVFDRYDEPEGFFPGQVLADLSV
                     SAALLAIIIVLSYLVPAPLTEPMDLSTTTYVPRPEWFFFFLDQWLIYFPGGALISIAD
                     VIVPGLFALLLLAVPWLDREPAHSLARRPFMVMISFLIIVAIVLNLFLALTRIANFPG
                     GTPSG"
     gene            complement(8437..9210)
                     /locus_tag="VFJ72_02695"
     CDS             complement(8437..9210)
                     /locus_tag="VFJ72_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET7478406.1"
                     /translation="MIARIRARTRETGRFLADWMEERTGIVSMTQKFLYEPVPKRGKW
                     LYTLGSATLFLITLQFLTGILLLFYYVPTTDNAWDSIYYIMNAAYFGQLIRGIHFWSA
                     NALLMVIGLHMVRTFLSASYKAPREMNWVVGVFLILIVTILAFSGYALRWDQEGFYAW
                     EVGTKIASYTPLIGNWMITSLLGGDTAGPATLSRVFALHVWLLPAALAPLIGIHLFLL
                     RKHGEFGAPYEYSERLAELRKRRQMEDLLAEEEDDANQE"
     gene            complement(9228..9860)
                     /locus_tag="VFJ72_02700"
     CDS             complement(9228..9860)
                     /locus_tag="VFJ72_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7478407.1"
                     /translation="MPEELQKDPLTRGQFLLFGALGTFVGAVMTIPPVVYFLDPAIKS
                     VLQGRSDIPDDWVELGSVFDIPAGKVVAQLVKFTQRQTYDSGLAGSKEGSIPNAVLLS
                     WKDGKMPELIKGKKKRLSGSDIEEINKELNVMNNACAHMGCPTRWSGEQKLILCPCHG
                     GMYDINGKHTAGPPPHGLWRYDFQVRGDGGLFVRHDFHLKGRGPGKPYVV"
     gene            complement(9876..11258)
                     /locus_tag="VFJ72_02705"
     CDS             complement(9876..11258)
                     /locus_tag="VFJ72_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478408.1"
                     /translation="MFPGAANVLALILQEGARGSFDLPSIQFPYLGTRAFVGIVVLIH
                     MFFAQLFIGYIIGSPILQAWGVRKGNERMQRLSRALDRFNVLTFSTGATFAGLFLVLL
                     VGFYPRVTAALFTHFFWFFPVMAMTAMVFTLYLLYVYHYRTHRRSLVIGLGAAFFILV
                     WQTILTGVDTFMVTGGGAGAVQSGGDLNNLGSALGGLISPLSVSLDLHRTFGNLSWPA
                     FAVAGWAGLRYRRAKRSEDRAFYDWAGSMGVLWGTVFLLFQPFVGFLNVLSLKSLGYT
                     PGNVSIPGNSGPYDRMVGAGSDTWTSHLLYINLLMVVALFVLSNAAMYMGAARHPERR
                     GRRPIQVFGLIAAVAGLYSVSPVAGLPVFWVRYVAMLVMVVGTVGALVAYVRAQRGFS
                     YGSPGGGYRAVLVLLGIAAVAVTMNMGYMKSNSRAPYTIYGQDKYQVSPERPFKESVL
                     QPRSGPGKTP"
     gene            complement(11261..11887)
                     /locus_tag="VFJ72_02710"
     CDS             complement(11261..11887)
                     /locus_tag="VFJ72_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478409.1"
                     /translation="MAKVGGYHTIVGIIPDGADLNQTVTDLNGIGIGGEDLTAILKRK
                     DPEVPEPFPEGTRYIVIPDDSRGLEIAVAFAAVFAVSGLLFAFTTPEIGAILFIFFIA
                     LAAILATATFTRVGVTPILMDIEAPAEESGFWNDEFERGKVLVFASTEDRRTLEAAWE
                     VFRRQNIYFDIIGGRLKPRPLSGAVLHHAAVRGDERPVEEAGQASGKS"
     gene            12381..12629
                     /locus_tag="VFJ72_02715"
     CDS             12381..12629
                     /locus_tag="VFJ72_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478410.1"
                     /translation="MATGLHMTLEEVRKAVQTRGGNLACPICASEEFVMEEATVLGAG
                     KQEAYGGHRLKRAQLICENCAFVINFDLARLERLAERR"
     gene            12726..13688
                     /locus_tag="VFJ72_02720"
     CDS             12726..13688
                     /locus_tag="VFJ72_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HET7478411.1"
                     /translation="MAAVSAPARGGWRSTAWGEYLAEFFGTFFLLLIGCSVVAETVAA
                     LNQSGRGTDISTAAGSGLWLVIVIGWALAVAFAVYIAGGVTGAHINPAVTLAFAVRRG
                     FPWRKVPGYMIAQVVGAFAGAALTYLVYHDAIASFEKANHITRGALGGAHDSTATYSI
                     FATFPAPYFASSLGPFIDQIVGTAVLVAVVFALVDERNQPPKSNVAPLMVGLVVAMIG
                     CAFGPNAGYAINPARDFGPRVFAWLAGWGQVALPGINYYFWVPIVGPLIGGVLGAFAY
                     DFFIGNVLKARGVEPTPDVESRGETDIDEAETEVEARGRTARDR"
     gene            complement(13790..14884)
                     /locus_tag="VFJ72_02725"
     CDS             complement(13790..14884)
                     /locus_tag="VFJ72_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7478412.1"
                     /translation="MPAPGREKRGSETLTTGAPLAVLLAAGLLIAYELLPVLELVAVA
                     MLLALVIRTVANRLHEAGLRPWMSVAVLLISFATFGSLVWLVVAPRILAEVSALAAAM
                     PGYADSLTHAARRLNEDVGFVPDLSHVADQIKGRAERGIESLPRLLAEVGHTATSALA
                     TFILAVFMAYDPGVLISGVLKLVPPGHHEATRRLMHALEKRLRGWIVGTGLAMIFLAT
                     GAGLGLWIVGAPLPITFALLAGLLNVIPYIGSIVGALLPALLALSISPLKALLVVVLF
                     VILNQIDGYVIQPMVMGHEARLHPVVVILSFLFLGKLLGFAGLLLSVPTAVFLMTVFD
                     ELGPKEGSKDAPGETAAKSPASRDARSSRS"
     gene            15160..16479
                     /locus_tag="VFJ72_02730"
     CDS             15160..16479
                     /locus_tag="VFJ72_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET7478413.1"
                     /translation="MPREGDEPLVRLAKTILDFNWTGEYTKPGPRLYPHQWSWDSALI
                     AMGYAHYDQERAESELKHLFEAQWENGLLPQIVFNPHFHDYFPGPGFWHAKESPHAPK
                     HRKTSGVVLPPVHATAVLRVYQRAEDGRGAKKFLAYAFPKLNAWHEYLYRERDPEDEG
                     LVYIRHPWESGMDNSPMWDAIMQRMQLRAGQIPEYRRADTHTVSAQDRPTSASYDRFA
                     YLVKFFAARNYDEAEIRADCPFLVQDVLFNSLLCRAGRDLAEIARILDEDPTPFEESA
                     KKTARAVNEKLWDEEHGIYLDYDLVSGCPIRVYFAPNFSPLYAGIPSEERAKRMLDSM
                     ENAGFGLSDEDITPVPSYDIRGFGFSPVQYWRGPVWININWFLMHGLRRYGFEEHAER
                     LRKTIIDLCGTEGFYEYFDPTTGQGHGSNLFSWSAALLVDVLMDERS"
     gene            16483..17250
                     /locus_tag="VFJ72_02735"
     CDS             16483..17250
                     /locus_tag="VFJ72_02735"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7478414.1"
                     /translation="MRLENKVTVVTGSSLGIGRAIALAFAREGASVTVDYRKHSDEAE
                     AVVKEVEELGGRALAVQADVSVPEDIRRLVQSAVDEFGRLDVMVNNAGIEEKMPFLET
                     PLDVWNRILSVNLTGAWLGCQEAAKQMVAGGGGGRIINVSSVHEDRPMPTNAPYCAAK
                     GGVRMLMRTIAVELAPHGITVNNIAPGAIETPINENLDEHPEQKEELLSEIPLGRIGK
                     PEEVANLALYLASDEASYVTGSTFTIDGGMMRHAGSL"
     gene            17247..18098
                     /locus_tag="VFJ72_02740"
     CDS             17247..18098
                     /locus_tag="VFJ72_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7478415.1"
                     /translation="MKRRFGVSQFTTTPWSFEQDVESYARLGVDAIEVCEDKLDGERF
                     AEQLALIGDHDMEISSVQPAVRTLFPSRTQPEPKDVPGRLALYRRAIERIVPFAPGSP
                     FVCNTGPPPDGNIQEVLDTAVKEYRGLADFAAEHGARIALEPLSPALMNVESAIWTLE
                     QAMRVIAAVDQENFGVCVDTWNVWQTPDVVEAIEACGDRIFVVHVSDWRTPRSFADRH
                     VIGEGDIPLPALLRAIHRSGYRGAHTLEIFSEGVPDSLWERDLPTIITESRAALDEAW
                     RQADEPG"
     gene            complement(18176..18823)
                     /locus_tag="VFJ72_02745"
     CDS             complement(18176..18823)
                     /locus_tag="VFJ72_02745"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7478416.1"
                     /translation="MAHWDRKFFESTRGRIVTMLRRTGRTVDELASELGLTGNGVRAH
                     LATLERDGLVRHGGYVRRGGSGKPAHAYELTPEARGLFPKGYEPVLGGLLDILAERAG
                     SEETVALLKETGARMAAGHESPSGDVRERLRAVVRVLDGLGGFAEIEENEGVFVIHGY
                     GCPLAALAEQHPQVCGLVEALIAELAAVRVRERCERGEDPRCCFEVRPADGVGRG"
     gene            18973..19485
                     /locus_tag="VFJ72_02750"
     CDS             18973..19485
                     /locus_tag="VFJ72_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET7478417.1"
                     /translation="MESRARMMGHPIHPILIPFPLGLLTTSVVFDIVHLITGSGQWAV
                     ISFWMIAAGVIGGLAAAVFGLIDWLGIPSGTRAKSVGLLHGASNFVMVALFAVSWLLR
                     LEAPGAPGIVAIALSFLGVGLAALGGFLGGELVVRMGVGVSEDANLNAPSSLSGRPAG
                     AGSSTARRVQ"
     gene            19670..21022
                     /locus_tag="VFJ72_02755"
     CDS             19670..21022
                     /locus_tag="VFJ72_02755"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET7478418.1"
                     /translation="MRAEEVLRDNDMGGWTRPASGLYPHQWSWDSAFIAIGLAHLDVR
                     RAALELRTLFAHQWKTGKVPHIVFNPAAPPESYYPGAEHWACAASSPDAPLAPPYTSC
                     LTQPPVHAIAVRRIYESAGREGEESLSHAREFLGEIYPRLFAWHRYLATYRDPEHSGL
                     VTIYHPWGSGADNSPRWDEPLAAVEVGNLPPYPRYDLQHVGDFSQRPTAADYDRYLWL
                     VGLIKRAGCDERTIYRSHPFLVKDVLSSAILVAANEALLEISEVAGAPDEDREIISEW
                     IQRGRRGLDACWDEDLGLCLDYDLRADRPLRVRTVAGLAPLIAGEQSPERLDRLVATL
                     DSPAFAGHAGLRWPVPPSTSPGEPGFRPRSYWRGPTWPVMNWLLWRALLLAGETRRAE
                     TLRRASLEQVAGEGFGEYFEPFTGEPLGSLRQSWTAAVVLDWLASGPSREPLDEARRK
                     "
     gene            21019..22539
                     /locus_tag="VFJ72_02760"
     CDS             21019..22539
                     /locus_tag="VFJ72_02760"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7478419.1"
                     /translation="MTGPGSGGGGSFWIETTPRTNFPVLDGDVAVDVAVIGGGITGLT
                     AALLLKRLGKTVAVIEARRVAEGVSGNTTAKVTSQHRLIYAWLIERHGERKARLYGEA
                     NEAAISLIASFVNEKKIECDLVRLPAYVFAETDAMVPQLRAEADAARRLGLPAGFVRE
                     VPLPFPVKGALRFDNQLQFHIRKYLLPLAEDIPGAGSHVFEGTRALRVREGLRCGVET
                     DRGTVAARDVVIATHVPFPFKGQFWGKAIPRREYGIAARTGSTPVSEGMFISAESPIR
                     SFRTAPRDGREVLIVVGESHKTGEEPDTERRYKKLEEWATSRFGVTEFENRWSTQDYF
                     PVDGLPFAGTIGAGSEHVYVATAFRGWGLSNGTAAAMLISDLICGRRNEWAGLYDSNR
                     KGPLISGSFYREGIEEAVHFVRDRLRASGRSPADLGTGEGRVVRVGGDTVAAYRDERG
                     EVHAVSAVCSHLGCIVSWNPAEKSWDCPCHGSRFDVEGNVLQGPATRNLPRKNVGP"
     gene            22809..23189
                     /locus_tag="VFJ72_02765"
     CDS             22809..23189
                     /locus_tag="VFJ72_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HET7478420.1"
                     /translation="MSPEIGNRRWAIAEGYIPGWSNGPEPQFTSHETACLLNTSDEDA
                     HVEITAYFSDREPAGPYRVKVPARRTAHVRFNELSDPEPIPADTDYASVIESDVPIVV
                     QHTRLDSRQAENALLSTVAYASHD"
     gene            23203..24984
                     /locus_tag="VFJ72_02770"
     CDS             23203..24984
                     /locus_tag="VFJ72_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HET7478421.1"
                     /translation="MPNTAEFMLNRLSEWGIKRIYGFPGDGINGLLGAFHEVGDRIEF
                     TQTAHEEIASFAACAHAKFTGEVGVCMATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQ
                     KRMGLGGHFQQEVDLLSLYKDVANEYVHYCMVPTQAAHLIDRAIRIAKSRRTVTCIIV
                     PDDVAEMDHQDPPRTHGATFSGEGAPNQPHVVPHEADIQRAAGVLNEGRKVAMLIGAG
                     AYGAAEEVREVADLLGCGVAKALNGKAALPDDLPYVTGSIGLLGTKPSNDMIEGCDTF
                     FMVGSSFPYSEWLPEPGQARGVQIDIDPTLIGIRYPMEVNLVGDAKETLRALIPHLNR
                     KEDRSWREEIEENVSRWWEIVENRAMEDADPVNPQRVFHELSPRLPEGCIVTGDSGSA
                     TNWWARQLKLRDGMMAALSGNLATMGPGVPYAIAAKFAHPDRPVIACVGDGAMQMIGN
                     NALIDVMKHWRRWSDPRLIVLVLNNRDLNQVTWEQRVLAGDPKLEASQNLPDFPFARY
                     AELLGFKGIRVDNPDGVAPAWEEALGADRPVVYEAVTDPEIPPLPPHIRFEQAQGMAS
                     ALSKGDPHEIRIAWNSLKGKLQEITTR"
     gene            24992..26080
                     /locus_tag="VFJ72_02775"
     CDS             24992..26080
                     /locus_tag="VFJ72_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7478422.1"
                     /translation="MGEKIPVERVEVSAFTIPTDSPESDGTLSWDSTTMVLVEAAGGG
                     KRGLGYTYGDVSVGKFIESKLASVAQGADAMSPPAVWAAMQHAIRNAGRPGVGAMAVA
                     AVDIALWDLKARILDLPLADTLPRFHDRAPIYGSGGFTSYSLDRLQEQLGGWASEGIP
                     RVKMKVGREPERDPERVRAALEAVGDGVELMVDANGAYTRKQALRWAGRFEEAGIPYL
                     EEPVSSEDREGLRLLRDRGPGGLAIAAGEYEWTLPDLADLAGCVDVLQADVTRCGGIT
                     NLLRADGICKGHQVPFSAHCAPAVSAHACCAMETLLHLEYFHDHVRLENMLFDGTLRP
                     EGGYLRPDPSRPGLGLQLKRSEAELFAA"
     gene            26110..29187
                     /locus_tag="VFJ72_02780"
     CDS             26110..29187
                     /locus_tag="VFJ72_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7478423.1"
                     /translation="MTDTVKREGYSLNDRQRMNLNALLLQSELRDAVKGEVRFDAGYR
                     AAYSTDSSNYRQAPLGVVVPRDPDDVVAALAVCRRHRVPVTPRGCGTSLAGQGTNAAV
                     VFDVSRYMREIVEVDPEKKIARVQPGVIRDQLAKPLEAEHGLSFPPDTSTHAYATFGG
                     MIGNNSCGAHSVMTGRTSDNVEEMDVVLYDGTRMRVGATSEEELERIIQEGGRKGEIY
                     ARLRDLRDRYGDRIRERYPQIPRRVSGYNLDELLPEKGFNVARALVGTEGTCAFVLEA
                     TVRLTPWPPARSILVLGYPSIFEAADHVPEVMSHAPMSCEAIDRELIDDMLRQGMHES
                     EVPLLPDGDGWLIVEFGAKTREESDARAREVMEALERDETTPRMRLFDDETEEAKLWS
                     VRESGLGATAYYPGGADHYEGWEDTAVPPEKFGHYLRDFQDLLDRYGYNTAMYGHFGQ
                     GLVHCRINFDLKSARGIRHWREFLDEAADLVVRYGGSLSGEHGDGQSRAELLGKMYGP
                     ELLRAFREFKAIWDPENKMNPHKIVEPYRITENLKLGADYDPPNVDTHFAYPQDGGSF
                     AHAAVRCVGAGKCRDTSTGTMCPSYMVTLDEEYTTRGRARVLFEMLRGETIKDGFKSD
                     EVFDSLDLCLSCKGCKGDCPVNVDMATYKAEFLSKYYESRIRPRYAYTMGLIMFHARL
                     AQYVPRLANFVTHAPVLKDVVRKAGGITTEREMPPFATQTFKTWFEERGGAANPAGPP
                     VVLFPDTFNNFLHPETAKAATEVLEAAGYQVIVPQQFLCCGRPLFDYGMLDTAKVFFD
                     RLIDGLRPYVREGVPIVGLEPSCVAAFRDELPNMMPNDEDARRLSRDILTLSEFLVQE
                     AKHYDPPKLNRKAIVHGHCHQKAIMGMSAEQQLYEKMGLDYEVLDSGCCGLAGSWGFE
                     EDKYDLSMKIGARRLFPAARDADTDTLVISDGFSCKTQLQQGTDRRALHTAQVVKMAL
                     DHGAGGAPRGERPESGYPDVILDGARPDLKTAAVAGAALALGAAALWVFRGRR"
     gene            29407..30408
                     /locus_tag="VFJ72_02785"
     CDS             29407..30408
                     /locus_tag="VFJ72_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7478424.1"
                     /translation="MSNGTGNGSEVVVITGASAGVGRAAVRAFAERGARMGLLARNPE
                     GLEGARREVEEAGGKAIAIPTDVADHEQVEAAAQRIEEELGPIDIWVNDAMTTVFAPF
                     KKISAADYKRATEVTYLGAVYGTMAALKRMLPRDRGTIVQVGSALAYRAIPLQAPYCG
                     AKHAIRGFTDSIRTELIHDRSNVRITMVQLPGLNTPQFDHCKTELPNHPQPVPPIYQP
                     EIAADAIVWAAHHKRREVYVGASSAITIVGNKFFPRLGDVYLGKTGYSGQQTSEPVGP
                     DRPSNLYETVPGDHGAHGIFDDQAHEKSPQLWATKNRRWIALTGAGALAAATLFLRR"
     gene            30413..30961
                     /locus_tag="VFJ72_02790"
     CDS             30413..30961
                     /locus_tag="VFJ72_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET7478425.1"
                     /translation="MHERATPPGFDKNPTRWSRRLLLAALALLGLFVSAYLALYQIGV
                     IGGVWDPFFESRRVLDFLGVPDAALGVLAYATEIILLFIGGRERWRTMPWTVLALGVV
                     ILSGAVVSVLLILMQAFLVGAWCTLCLASAAISLAIFLLGADEPVAGPRYLKRVRGSG
                     GSAWRALWGRGDSREKRRRSHA"
     gene            30954..32765
                     /locus_tag="VFJ72_02795"
     CDS             30954..32765
                     /locus_tag="VFJ72_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7478426.1"
                     /translation="MPESPPYPPIRDYALIADSNSVALVSRAGSIDWCCIQRIDAGSC
                     FGRLLDWQEGGFCSISPAAGSGDVSRRYLDETLVLETTFRVGGGVARLYDFYALPAST
                     EEHPYRQLIRIVEGVSGSVELDLEIMPRFDYGSMKPWIRQEGVRVYSAIGGNDGLLIS
                     SDADIAQTDEHALRAAATVRAGERVRLSFTSVPPEQLDYDRPEALGANEMDRRLEETI
                     AWWRDWSSAIRFEGQRKSSVLRSAITVKALMNDLTGAIAAAATTSLPENPGGSLNWDY
                     RYSWIRDSFFSVRSLAEVGFDPVADRFRRFVERSSAGSADQLQLMYGMGGERRLTEET
                     LDYLEGYRGARPVRVGNAAAGQLQLDMYGELLELSWRWHQRGNSPDDDYWRFLMELVD
                     TAAERWPEPDSGIWEKRGDQRHFVHSKVMCWATLDKGLRLAKECMRKAPERRWKKTRG
                     EIRDAIESEGYDKQRGVFVQSFGAKDLDAALLLLPRVGFVDYKDERMVRTTDAIRDEL
                     DDDGLLKRYRIDDGEQEGAFLACSFWLVECLAHQGRVEDARAVFDRTLATGNDLGLFA
                     EEYDTVNGELLGNFPQGLTHLSHIAAAVALQKHESTV"
     gene            32806..33360
                     /locus_tag="VFJ72_02800"
     CDS             32806..33360
                     /locus_tag="VFJ72_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HET7478427.1"
                     /translation="MYGGKRGGLRGWLVDRLGGGGESIYTFFACVLAMAVSGFGAYLT
                     KQPLLFPSLGPTAFLFFESPMSAQSSPRNTLIGHAVAVLAGAASLAVFGLLHHPSVLV
                     EGVSPARVGAGALSLALTGSVLLLLKSSHPPTGATTLIVSLGLLKTPRQMAVLMAGVV
                     LLTVVCWIVNRAAGVPVPPWSSRE"
     gene            complement(33403..34110)
                     /locus_tag="VFJ72_02805"
     CDS             complement(33403..34110)
                     /locus_tag="VFJ72_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7478428.1"
                     /translation="MARVLVVEDDAAVRDVVGHALGREGMEIEAVASGEEAMERFSSA
                     SPFDVVILDIMLPEMDGISVCQELRKGGSPSANAPVIMLTARDDETSVVVGLEVGADD
                     YVTKPFRPRELVSRVRAHLRRRRLDSASVPAERSTLEFPNLKIDLFGRQVFVRGDEVD
                     LTAKEFDVLALLAAHPGRVYSREQIMRHLWNGEFFGEARAADVHIQHIRKKIEPDTTK
                     PRYIQTVRGAGYKFANV"
     gene            complement(34103..35830)
                     /locus_tag="VFJ72_02810"
     CDS             complement(34103..35830)
                     /locus_tag="VFJ72_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7478429.1"
                     /translation="MPAVAVLIVMLAIGTILLYGLQAVKTRLGEYSREHALAEAAAAA
                     TAVESASPDGAGDALKLAVKSSGGEALLVDRKGKVLAREGSGLPSALPQRVIDTAARG
                     NRLNERIEDHRVTTVPVVRGGGLRGGLVFVSDNSESAVLDIFSRSNVEAAAIAAVLGG
                     GLMLLVATLLSRRVERLTLGAKAIERGDFSSRIEPGYGDELGELAETFNAMTAKVQKS
                     MSRLEENNATLDAILDNLNEGVLATDLKGRVMFANLSARRMLGMTSERVQDVRQPEEL
                     PDPFGDFDLPDAVARCARRQECGEARAQGDETFFHINLEHMPAFDEHRGGVLVVIRDL
                     SESRRLEANQQRFLANAAHELRTPITTILGASDLLLTEEEDDPRVRERFLKHIRSEAE
                     RMQRLSETLLRLARTGWDRRAPNLREVDLGTVAGRVAERMRPLARGAGLTLTVEGEGG
                     RVRADEEWLEQALLVLTSNAVQHSERGGRVRLRTHGPVVTVEDEGVGIEEEDIPHVFE
                     RFHRGKDSEGFGLGLPICKELVESMNGEISLSSRKGKGTTIEIKLPEIRARTGPQAAT
                     QAATEDQNG"
     gene            36037..36501
                     /locus_tag="VFJ72_02815"
     CDS             36037..36501
                     /locus_tag="VFJ72_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478430.1"
                     /translation="MRSGGSIRWRGVGAGALAAALTVFLLSPMVRGLYDILTGSLAPM
                     GKLTSGLIVVALVSGFLAYLFGGFVAGRLARGAGGANGAMTAVMGTAVGALLGAVGIA
                     VSGNVWVAIMNLGSAGLAVLIGLLLLLIDLFGGYIGGKLGEPSEPRMHRLHD"
     gene            36570..37598
                     /locus_tag="VFJ72_02820"
     CDS             36570..37598
                     /locus_tag="VFJ72_02820"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7478431.1"
                     /translation="MQIGTHTFLPTERVHFGAGSLRKIEEETGRKGRAFIVTGRSLNE
                     KTDLIRRVEEMLGGRHAGTYAAISQHTPGSAVEEAASEAEMAGADLLISIGGGSVIDG
                     TKAISRQLGYPSQVAVPTTLSAAEWAHRVGVTDEAAGKKRGFADPKTVPPVVILDPET
                     TVYTPERLWLSTGIRALDHAVEGFLYGGPHPVTDVTGLEGARRLMEYLPISKREPEDL
                     EARAELQVAAWLSYFGPANTPMGLSHELGRRIGASYGVAHGITSCITLAPSLEVVRER
                     IPKDRWARLGEALGGEPAERVADLVRELGLPGRLRDVDVPRGEIERIASEYGEREADA
                     REILTRAW"
     gene            complement(37608..39200)
                     /locus_tag="VFJ72_02825"
     CDS             complement(37608..39200)
                     /locus_tag="VFJ72_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET7478432.1"
                     /translation="MSDIEFLILLLGAVAVLAQLARITNVPYPIFLVLGGLAIGLAPG
                     LPKIELAPEVIFLVFLPPLLNSAAFTASPRDLRAHLRPIAMLAVGLVLATTLAVAAVA
                     HFVVGLPWAAAFVLGAVLAPTDPVAAEAIFRRLGVPATVSTVVGGESLVNDGSALVVY
                     RVAVVAVVSGAFSATQAGLNFVVVSAGGLLVGLVFARLMLPLWARLKDALILIMTSVL
                     LPYVVYILAEEVLHLSGILAIVSYGLYQGWHAPRLFPDASTRLQALSFWSVLTFVLES
                     LLFILVGLQFTSILEGLDAGSTWGTLLLYAGLVYAAVALTRLVWFFTTPYLHPVFDRL
                     LHSTYLRAPWQERLVMSWSGMRGAVSLAAALAVPLTVQSGAPFPGRDQILFLTYGVIL
                     ATLVLQGFTLPPLIKSLRLKGDGEDSKIMELEARLHAARAALEKLDLLCAVERVPPHS
                     QQQMRESYEQRIRRYTAGIEAGGATEEYNQDSSAWRNWRRQLIAAEREAIVGLRDAGA
                     ISPEIMRRIQHDLDLEESRIGG"
     gene            complement(39208..>39596)
                     /locus_tag="VFJ72_02830"
     CDS             complement(39208..>39596)
                     /locus_tag="VFJ72_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478433.1"
                     /translation="LSLPALIRGLGLEDDGSTQREEVTGRIEVAQAALDRLEELVEEE
                     WVREETAERVRGLYNYRRGRFAARFDGDSDSLEERSTAYQRLMIELLRAQRRTLVRLR
                     NEGEISDDAMHRIERDLDLEESRLEI"
BASE COUNT         7011 a        13025 c        13217 g         6343 t
ORIGIN      
        1 tacgacgtgg acacctggca gtacgccggg atggaggtca gcggctcggc gggcaagcgc
       61 gagcagggcg gcgtgaccgg agagcaggcc cactacgcgc agggcggtca gctagaacac
      121 ggcgaaccca ccgagatgga cgagtcgctg gagcacccgc gctgcgtctt ccagctactc
      181 aagaagcact accgcaggta tacgccggag gcggtggagc ggatctgtgg ttgtcccaaa
      241 gagaagttcc ttgaggtggc cgagaccctc tgcgagaact ccgggcgcga gcggacctcg
      301 gcgttctgct acgccgtcag ctggacccag cacaccgtcg gcgtccagaa catccgggcg
      361 gcgaccatag tgcaacttct gctaggcaac atcggacgtc ccggaggcgg catcatggcg
      421 cttcgcggac acgcctcgat ccagggctcg accgacatac ccacactcta caacatcctc
      481 cccggctaca tcccgatgcc ccacgcccac gcccacgcgc accaggacct cgacgagttc
      541 atcgagaaga acgccgccaa gaccggttac tggggcaaca tgcgggccta catggtgagt
      601 ttactcaaag cgtatttcgg cgatgcggcc acggaggaga acgactactg cttcgaccac
      661 ctgccacgcc tcaccgggga ccactcgatc tacccggcga tcatgggcat gatggacggc
      721 tggtgcaagg gcttcttcgt catgggagag aacccggcgg taggcgccgc gaacggcaag
      781 ctccagcgtc tcgccctcgc caacctcgac tggctcgtcg tccgggactt ctcggagata
      841 gagacggcct ccttctggta cgactccccc gagatagaga ccggcgagct gaagaccgga
      901 gagatcggca cggaggtctt cttcatgccc gccgccgccc acacggagaa ggacggctcc
      961 ttcaccaaca cgcaaaggct cctgcaatgg caccacaagg ccgtcgagcc caaaggagac
     1021 tgccgctcag agctgtggtt catgtaccac ctcggcagga aggtgaggga gaagctcgcg
     1081 aactccaccg atcccaaaga ccgggccgtc ctcgaccttg cctgggatta tcccacgcta
     1141 gggacgcacg aggagccgga cgccgagact gttctgcggg agatcggcgg ctgggacgag
     1201 aacggcgacg cgctcgagag ctacctgccg ctcaaggacg atggtacgac gacttgcggc
     1261 tgttggatct acgccggctg cttcgccgat gaggtgaacc agaccgcccg ccgcaagccc
     1321 ggctccgagc agagttgggt cgcccccgag tgggcgtggg cctggccgat gaaccgccgc
     1381 atcatctaca accgcgcctc cgccgatccg gaaggcaggc cctggtccga gaggaagaaa
     1441 tacatctggt gggacgagga gcaggagaag tggaccggcg aggacgtgcc ggacatcagc
     1501 ccgaccaagc gtccggacta cgagccaccc gacgacgcta ccgccgagga cgccatccgg
     1561 ggcgacagcc cattcgtcat gcaggcggac ggtcggggtt ggatcttcgt ccccgccggc
     1621 ctgaccgacg ggcccctccc gacccactac gagccgcacg agtccccgtt cgagaacgcc
     1681 ctctacggac agcagtccaa cccggcgcgc cagcagttcc cgaggggcga caacccgtac
     1741 aacccctctg acgggcaatc cggcacgcgg gtcttcccct acatcctctc gacctaccgc
     1801 ctcaccgagc accacacggc gggcggcatg acccgcacgg tcgcgcacct ctccgagctc
     1861 cagcccgaga tgttctgcga ggtgagcccg gaactggcgc aagagcgggg cctggagaac
     1921 gggggatggg cgaccatcgt tacgacgcgc tccgccatcg aggcccgcgt gctggtcacg
     1981 gaccggctgg cgcccctgcg ggtgcagggc aggacggtac accaggtcgg gatgccctac
     2041 cactggggta gcaagggtct cgttacgggc gactcggcca acgacctgct cggcctcgtc
     2101 ctcgacccga acgtgcacat cggcgagtac aaggtggcga cctgcgacat ccggcccggc
     2161 aggaggcccc gcggtcccgc cctcgcccgg ctggtagacg actaccgcgc ccgggcaaac
     2221 gcccacgcgg agaacggagg tgcgagagcg tgagcgagac caacgtcatc cccggctccg
     2281 gcagcccgga cgcctttgac atccagggcc gggagacact cgagacgcgg cccgtgatcc
     2341 cgctggggca gaagggctac gaggacggac agccccgcat gggcttcttc accgacacca
     2401 ccgtctgcat cggctgcaag gcgtgcgagg tcgcctgcaa ggagtggaac gacgtccccg
     2461 ccctgccctt cgagcttacc ggcgactcct acgacaacac gggagcttta ggagccgact
     2521 cctggcgcca cgtcgccttt atcgagcagc gcaagccggt aaaggtcgtc caggacgcgg
     2581 ggttgacaac ccccgcatcc ggcgacacgg acttccggtg gctgatgagt tccgacgtgt
     2641 gcaagcactg cacggacgcc ccgtgcctgg acgtgtgccc gaccggctcc ctcttccgca
     2701 ccgagttcgg taccgtcgtc gtgcaggagg acatctgtaa cggttgcggc tactgcgtcc
     2761 cgtcgtgccc gtttggagtg ctggaccagc gccaggacga cggacgggtg tggaagtgca
     2821 ccctctgcta cgaccgtctg aagggcggga tggagccggc ctgcgcccag gcgtgtccga
     2881 ccgactcgat ccagttcggc cccctcgaag aactgcggga aaaggcgaac taccgcctgg
     2941 agatggtcca tgaggcgggc gtaaccgacg cgaggctcta cggggccgac gaggaggagt
     3001 acgggatggg cgccttcttc ctgctgctcg acgagccgga ggtctacggt ctgccgcccg
     3061 acccggaggt cgtcacccgc aagcttaaga gcttgtgggg tccggcggcg gtcgcggcgg
     3121 gtgcgatggc tgtcggggtg gtcgccgcct tcgcgggaga gggccgatga acgcgcggcc
     3181 cgaggtatcg gtctccgaag aggctcgcca cgatggccgt gggaccggcg cagaagcgcg
     3241 ctcctactac gggcggccgg ttttaaaaga gccggtgtgg agccgcgaga tcccctggta
     3301 cttcttctcc gggggactgg ctggcgcctc cgcgagcctg gccgcagcgg cgcggctagc
     3361 gggcaacgtg gagctcgcgc ggcgggcgcg gctcgtggtg ctcgctggcg cagcggtaag
     3421 cccggtgctc ctgatctccg acctcggtaa acccgaacgc ttctacaaca tgctccgggt
     3481 cataaagccc acctccccga tgagcgtggg gacctggatc ctctcggcct tcgggacctc
     3541 ggtggggatc gccgcggcga gcaaggtgct cggcgtcttc ccgcgcctcg ggcggctctc
     3601 cgggacggcg gcggccctcc tcggcccggc gctctcgacc tacacggccg ttctcatcgc
     3661 cgacacatct attcccgtgt ggcacgaggc gcggcgggag atgccgttca tcttcgccgc
     3721 gagctccgct gcgagcgccg gcgccgcggc cgcgatcctg acgcccgtcg aagcagcgag
     3781 acccgcgcgc cgtctggcag tcggaggcgc gctggccgag atcgcgggtg tagaggttat
     3841 gaagcggaga ctgggcaagt tgctcgcgga gccttacgag aagggcgaag ccggccgcta
     3901 cgacaggctc gccacggctg caaccggtct cggggccgca gtgatgggga tgttcggccg
     3961 caggcaccgg tcggccgtgg tggcgggcgg cgcccttgtt ctggcgggcg ccgccctgga
     4021 gcggtggtcg atcttcaagg ccgggttcca atcggcgcgg gaccccagat atactgtcat
     4081 accgcaacgc gagcgcagag aatcccgcgc tcgcgcggat ggataggagg ctttcgccac
     4141 gggcgcgctg ctcctgattg gaaacgtcga ttcgctggaa agacggcgat gaggaccttc
     4201 gcgagtacgt tggggataat agcgctgatg gctgctgccc tggtcgggtt cctcgcggtc
     4261 gctggggtgc tcagcgaggc gtccacgagc acccaggttg ggggcgcggc ggcggacgcg
     4321 gccgagagct tacccgccgc ggagacgacc tccgtcacgg tagacaagtc ggctacggcc
     4381 ggcaagctaa agtggaccat ccacgacgcc agccggatgg ccgaaatacg caagttcacc
     4441 atcccgtcga ccaccaagcg gggcgacttc gtgcgcgtga cctttaccgt caagaacgtc
     4501 tcgaagacac ccgtaacgct cgatgcccgc tcgctgactc tcatagacaa gacgggcttc
     4561 aaggactatc ccgccgcagg tgtaaacagc gagtacatcg tgccctcgaa ggcgatcctg
     4621 ttcaacgatg aagggttgct ggaccctggc gaggagagaa acggggaagt gaacttcgac
     4681 ctggcggtgc cgttcggcgc ccatccttcg ggcctgcgtc tcggggatac cgggacctcc
     4741 ggcttccatc tcgagctggg agacgccgag ccgaccgccc accacgagaa aaccgtaaag
     4801 ctagggtttt gagcgcgacg tgaagagccg cgaacttccg gagactggag gccgctagac
     4861 gtgttcgatt cgctctcact accgctcttg atactcgtct tcgccggggc tgctggggct
     4921 gtatgggtgg caggcgtggg tctctcggat accacggacg tgctctcgca gcgcttcggt
     4981 ctcggcgagg cgctgggcgg cctgatcatg ctggccatcg tcacgaacct gccggagatc
     5041 gccatcaccg cgagcgccgc cctctcaaat aacctcggag tggccgtcgg caacatactc
     5101 ggggggatcg ccatccagac cgtcgtgctc gcggcgctcg acgttttcgg cgtgcgcggg
     5161 agggagcccc tgacctaccg ggcggcatcc ctgatgctcg tgctcgaagg ggcgctggtg
     5221 gtggccgtgc tgaccgtcgc cgttatgggc agccagctcc catcgtcgct gatcttctgg
     5281 cgcctcgaac ccggcgggct cctgatagcc gccctgtggg tcgtggggct gctcctcttg
     5341 aaccgcgccc gttccggcct gccctggcag gagaagggcg aagcgcccga aggacaggag
     5401 aagcctcgcg gccactccaa ggcccacaaa gaccggcagg cccacagcaa gggcgtcggc
     5461 accggcaggg cggccatcat cttcgcggtc gcggcggtcg ttacgctggt cggcggcgtc
     5521 gttctggagc agagcgggga gaacatagcc ggcaatatcg ggatgagcgg cgtgctgttc
     5581 ggctcgacct tcctggccgc cgcgaccgcc ttacccgaga tctccacggg gctcacctcc
     5641 gtcaggctgg gcgatttcaa gctggcggtg agcgacatct tcggcggcaa cgccttcctg
     5701 cccgtgctgt tcctgatggc gagcctgctc tccgggcagg ccgtgctgcc gcaggcccac
     5761 gacaccgaca tctacctgac ggggctcggg atactgctta cggccgtcta tatctggggc
     5821 ctcgtcttta ggccccggcg ccgggtgctg cgcatgggcg tggactcgct ggcggtgctc
     5881 ctcctgtacg tagtcgggat cgcggggctg gtagcggtcg ccctcggcgg gacaggctag
     5941 acgggacccg ctacggaccg gggaagagac gttatagagc cacagaagcg ctcaccgctg
     6001 aaccgatacc tttccagcct gcgcggtgtc gtaggcgcca gcacgaccgc ctacgggttc
     6061 acgctcacga tatggtccac cggctcgatc ctgagccatg cttacgggct gccgagcccg
     6121 ccgcaggtgt tcatgttctt cggtggcgcg atagtcgcct tcgccatggt ggcggttctg
     6181 gcgttcggcg gtgtggcgtc ggaggtcggc gggtccaacg acatcaaggc gtggggcagc
     6241 ttccgcttcc tcttggtggg gctggcggtg gggtcggcct ggctggagtc cgcctacgtg
     6301 ccgagccttc cggggtggcc cgtcggggcg ttcctggcga cggccgcgta cctcaccatc
     6361 acgggagcgg aaaactccgc ggccggaaca ggagaaagcc gcgagtcctg accggcacgc
     6421 cagatagagg ccggccttac tcctcttctt ctccctttac cagccagtga aggatcagga
     6481 cggctactcc ggtggtcagc aggatcagga agaaccccag gaagaaacgg acgagcgggg
     6541 ggagcaggct caactcaagc atcatggggt ctcccggggc gcggccgagg gacaaccctt
     6601 ctggacgaac ttctggtcct ttagcgttat gcagttgtgg accagccatc cgtcgctgcc
     6661 gttgcccgcc cggcgcgcgg tctcgcgagc gtatcccatc gtcatcatgg tgaagacgac
     6721 cgttacggcg cagagcagca gggcaaactg gctccagcgg ctggcccggc cccagtaaaa
     6781 gccggtcgcg gaggctctca ggtagagcgc cagcgcgaag aggcccacca ccgcgaggcc
     6841 gatgagaccg atgtacttgt aagggtacat ggcgccgagc ggtatcggcg gtttgtcgaa
     6901 cagggcgatg ttcgggttta cccgttcgcc gaagaccaga ccgatctggg gcaccaggta
     6961 caggtcggcc gggatcatgt tcagtagccc gaagagcgtc acgaacccga gggcgcccat
     7021 cgtcagcttc ctcagccccg gcacgggctt tacggcgaac ttgagcttgt ggacgaagta
     7081 ggcgacggag gcggcgccca tgatcagtag ctccgttacg agcatgttga agagccagga
     7141 cttgtccccg agcatcaggt acttgaaggc gtccggcgag gcggacttta tctccgtcat
     7201 gtactcgtag cccatgaacg gctgcaggag caggaaaccg aagccccaga taatgcccca
     7261 gtgtcccatc cagtcgtagt actcgcgatc ttcctcggtg gtggagcgga ggtagcgcca
     7321 cgctccccag ccggcgatca ggaaccccgc gtaggagaag ttcccgacga agcggtgcat
     7381 gttgagcgcc atgtaggtcg ggttcatgaa ggtccagccc acgttggacg cctgggcgtt
     7441 tgcgggggtc agcatgtagc tggcgaccac gttgatgaag atcatctgcg tcagcccgaa
     7501 gaggccggct atgaggccga aggtaacgtg caggttcttt cggtaggcca tcgtgtccca
     7561 cgagtggtac caggcgtaga ccatgatcac ctcgcccacg aacatgaacg cttccgccag
     7621 aagcacccag aaaaagacgt tctgcatgaa gctccagaag accggataga gcgtgatcag
     7681 ggagagtatg aacgtgaagg cgatggcgct ccccacgccg aacaggagca gcgtgaactt
     7741 ggccgcgttc ctggcgaagc ggtcgtagta gggccgtttg gtcatcgagc cgatccactc
     7801 gcttatggct ccgatcaggt tgatcccgat gacgaacgcc gcgatcagga tgtgcggcat
     7861 taccaggagc ggtatcacca ggtccttacc tattaacggt atctgcaggt cgccaatcgg
     7921 cacttttacc tcctgctacc cggacggggt cccgccgggg aagttggcga tgcgggtcag
     7981 ggccaggaac aggttgagca ctatcgcgac gattatcagg aagctgatca tgaccatgaa
     8041 cggtctcctg gccaggctgt gagccggctc ccggtcgagc cagggcaccg ccagcagcaa
     8101 cagggcgaag aggcccggca cgatcacgtc ggcgatggag atgagcgcgc cgccggggaa
     8161 gtagatgagc cactggtcca ggaagaagaa gaaccactcc ggccggggca cgtaggtcgt
     8221 cgtggagagg tccatcggct ccgtcagggg ggcgggcacc agatatgaca gcacgatgat
     8281 tatggcgagc aacgccgccg agacgctcag gtccgcgagt acctgtccgg ggaagaaacc
     8341 ctccggctcg tcgtagcggt cgaagacgtt ctcctccgtg atctcctctc cctcctctac
     8401 cgctttcggc ggcccgtgcc ttatctccga catccgttac tcctggttcg catcgtcttc
     8461 ctcctctgcc agaaggtctt ccatctgccg gcgtttgcgg agctccgcga ggcgctcgga
     8521 gtactcgtag ggggcgccga actcgccgtg ttttcgcagc aggaacaggt ggatgccgat
     8581 gaggggcgcg agcgccgcgg gcaggagcca gacgtggagg gcgaacacgc gggagagcgt
     8641 ggccggaccc gccgtgtcgc cgccgaggag gctcgttatc atccagttgc cgatcagggg
     8701 cgtataggag gctatcttgg tgccgacctc ccaggcgtag aagccctcct ggtcccagcg
     8761 cagggcgtag ccgctgaacg cgaggattgt cactatgagg atcaggaaca cgccgacgac
     8821 ccagttcatc tcgcggggcg ccttgtagga ggcggagagg aacgtcctga ccatgtgcag
     8881 tccgatcacc atcaggagag cgttggccga ccagaagtgt atgccccgga tcaactgccc
     8941 gaaataggcg gcgttcatga tgtagtagat tgaatcccac gcgttgtccg tcgtcggcac
     9001 gtagtagaag agcagcaata tgcccgtcag gaactggagc gttatgagga agagcgtggc
     9061 gctccctaga gtatagagcc acttgccgcg cttgggcacc ggctcgtaga ggaacttctg
     9121 ggtcatcgag acgatgccgg tcctctcctc catccagtcc gcgaggaacc ttcccgtctc
     9181 cctggttcgc gctctgatcc tggctatcat ccgtcggcct cctcgtccta gaccacgtaa
     9241 ggcttgccgg ggcctctgcc cttcaggtgg aaatcgtgcc gcacgaacag gccgccgtcc
     9301 ccgcgtacct ggaagtcgta gcgccagagg ccgtgcgggg gagggccggc cgtgtgcttg
     9361 ccgttgatgt cgtacatccc gccgtggcag gggcacagga tcagcttctg ttccccactc
     9421 cagcgtgtcg ggcagcccat gtgggcgcag gcgttgttca tgacgttcag ctctttgttg
     9481 atctcttcta tatcgctccc ggagagccgt ttcttcttgc ccttgatgag ttccggcatc
     9541 ttgccgtctt tccaggagag gagcacggcg ttggggatgg aaccctcttt ggagcccgcc
     9601 agcccggagt cgtaggtctg tcgctgggtg aacttgacga gctgggcgac gaccttgccg
     9661 gcggggatgt cgaagaccga accgagttct acccagtcgt cggggatgtc cgagcggccc
     9721 tggagcacgc tcttgatcgc cgggtccagg aagtagacga ccggcggtat ggtcataacc
     9781 gccccgacga acgtgcccag ggctccgaac aggaggaact ggcccctcgt cagtgggtcc
     9841 ttctgtagct cttcgggcaa ggtctggctc tccttctagg gtgtcttccc gggaccggag
     9901 cgcggctgga ggacgctctc cttgaagggc ctctccgggc tcacctggta cttgtcctgg
     9961 ccgtagatcg tgtacggcgc gcgagagttg gacttcatgt agcccatgtt catcgtcacg
    10021 gcgacggccg cgatcccgag cagcaccagc accgcgcgat acccgccgcc cggactcccg
    10081 tagctgaatc cccgctgcgc ccgcacgtag gcgaccaggg ctcccacggt gcccacgacc
    10141 atgacgagca tcgccacgta gcgcacccag aacaccggta agcccgccac cggtgacacg
    10201 gagtacagac cggcgaccgc cgcgatgagc ccgaagacct gtatcggacg ccggcctctc
    10261 ctctccgggt gtctcgcggc tcccatgtac atcgccgcgt tcgagagcac gaagagcgcc
    10321 acgaccatca ggaggtttat gtacaggagg tgggaggtcc aggtatcgga cccggcgccc
    10381 accatcctgt cgtagggacc ggaattgcca gggatcgaca cgttgccggg ggtgtatccg
    10441 agggacttca gcgagaggac gttgaggaag cccacgaaag gctggaagag caggaagacc
    10501 gtgccccaca acacgcccat tgaaccggcc cagtcgtaga aggcccggtc ctcggatctc
    10561 tttgcccggc ggtagcggag acctgcccag cccgcgaccg cgaaggcggg ccaggagaga
    10621 ttgccgaagg tgcggtgcag gtccagcgac acggacagcg ggctgatgag accgcccagc
    10681 gccgagccga gattgttcag gtcgccgccg ctctggacgg cgccggctcc tcccccggta
    10741 accatgaagg tgtccacgcc ggtcaggatg gtctgccaca ccaggatgaa gaacgcggcg
    10801 ccaagaccga tcacgaggct ccggcggtgc gtcctgtagt ggtagacgta gagtagatag
    10861 agagtgaaga ccatcgcggt catcgccatc accgggaaga accagaagaa gtgggtgaag
    10921 agggccgccg tcaccctggg gtagaacccg acgagcagca cgaggaacag ccccgcgaac
    10981 gtggccccgg tgctgaaggt cagcacgttg aagcggtcga gcgcgcggga gaggcgctgc
    11041 atgcgctcgt tccccttgcg gactccccac gcctgcagga tgggcgagcc gatgatgtac
    11101 ccgatgaaca actgcgcgaa gaacatgtgg atgagcacca cgatgccgac gaaggcgcgg
    11161 gtccccaggt aggggaactg gatgctcggg aggtcgaagc tgccacgggc gccttcttgg
    11221 aggatcaggg cgaggacgtt cgcggcgccg gggaacatct tcagctcttc cccgatgctt
    11281 gcccggcctc ttcgacgggc cgctcgtctc cgcgcaccgc ggcgtggtgc agcacggccc
    11341 cgctcagcgg ccggggcttg aggcggcccc cgattatgtc gaagtagatg ttctgcctcc
    11401 ggaacacttc ccacgcggct tcgagggtcc gccgatcttc tgtagaagcg aagacgagga
    11461 ccttgcccct ctcgaactcg tcattccaga acccggactc ctcggcgggc gcttcgatgt
    11521 ccatgaggat cggcgtaacg cccaccctgg tgaacgtcgc cgtcgcgagt atcgccgcca
    11581 gggcgataaa gaagatgaag aggatcgccc cgatctcggg ggtcgtgaac gcgaacaaaa
    11641 gccccgatac ggcgaacacc gcggcgaagg cgacggcgat ttcgaggccg cgggagtcgt
    11701 cggggatgac gatgtagcgc gtcccttccg ggaagggctc cggcacctcc gggtccttgc
    11761 gtttcaggat ggcggtcagg tcttctccgc cgatcccgat tccgttgagg tccgtgacgg
    11821 tctggttcag atcggccccg tcggggatta tccccacgat ggtgtggtat ccccctactt
    11881 tagccaaggc aggttctccg gccggtgaag gtgcgccatg aatcgtacag gcttcctggg
    11941 ctgactagag taaacacgga cccgcctcgc aaactccttt cccgtcatcc ggttttcccc
    12001 cggatgagcg gctacgaatc ctcctcacgg tgtgaacttt acgggatcgc acgcccgagc
    12061 ggtagccgtc cgggccgcag gccgcgaaat cttaatcaag tccttacaac acgctaatcc
    12121 gatgaccggt gaacgccggg ctgtttctgg gcgcaacgca aggcttaacg gaaggtttgc
    12181 agagccataa cgaaagcttt actggaagtt taaatagcga atgtatcatg aattatggtg
    12241 aaggagcgag gatcggtgaa agactatctg gagcggcgga agggagtgga tattacacag
    12301 actgttacac agactgacag gtgtataagc gccgcagtcg gcggtagccg gttgcggcag
    12361 atgagaagga ggtgcgcggc atggctaccg gactacacat gacgctggag gaggtgcgaa
    12421 aggcggtcca gacgaggggt ggaaaccttg cctgccccat atgcgcgagc gaggagttcg
    12481 tgatggaaga ggcgacggtt ctgggtgcgg gaaagcagga ggcgtacggc ggtcaccgcc
    12541 tgaagcgggc gcaactgata tgcgaaaact gcgccttcgt gatcaacttc gacctcgcta
    12601 ggttagagcg gctcgccgag cggcggtgag gtcgtccaca cgcgcaacaa ccctgtaacg
    12661 cgaacacccc tgccgttgta gcggcgcgag cgaggggtca gaaagcctag agagaaagga
    12721 cggtcatggc agcagtatcg gcaccggctc ggggaggctg gcgatctaca gcatggggtg
    12781 agtacctggc ggagttcttc ggcaccttct tcctactgct gataggttgc tccgtcgtcg
    12841 cggagacggt ggcggccctc aaccagtcgg ggcgcggcac ggatatctcg accgccgccg
    12901 gcagcgggct gtggttggtc atagttattg gctgggccct ggcagtggcc ttcgccgtgt
    12961 acatcgcggg cggggtgacc ggggcgcaca tcaacccggc cgttaccctg gccttcgcgg
    13021 tcagacgggg cttcccgtgg aggaaggtcc caggctacat gatcgcgcag gtggtcggcg
    13081 cctttgccgg ggccgcgctg acttacctgg tttaccacga cgccatagcg agcttcgaga
    13141 aggccaacca cataacccgt ggagcgctcg gcggggccca cgactccacg gccacctaca
    13201 gcatcttcgc caccttccct gcgccgtact tcgcgagcag cctgggaccc tttatcgacc
    13261 agatagtcgg aacggcggtc ctggtggcgg tcgtcttcgc gctggtggac gagaggaacc
    13321 agccgccgaa gtcgaacgtg gcccctttga tggtagggct cgtggtggcg atgatcgggt
    13381 gcgcgttcgg gccgaacgcc ggctacgcca taaacccggc gcgcgacttc ggaccgaggg
    13441 tgttcgcgtg gctggccggg tgggggcagg tggccttgcc gggcatcaac tactacttct
    13501 gggtgccgat agtcgggccg ttgatcggtg gggtactcgg cgcgttcgcc tacgacttct
    13561 ttatcggaaa cgtgctcaag gcccgtggcg tggagcccac gccggacgtg gagtccaggg
    13621 gcgagaccga catagacgag gcagaaaccg aggtcgaggc ccgagggcgg accgcgaggg
    13681 acaggtagcc tcactgggca aaccccatac ttaccatcag ccgggcgtaa agcgcgacca
    13741 gcgggccata gaccgcgagc ggggcagcgc ctcacttctt ctgctcgagc tagctgcgcg
    13801 atgatcgagc atcgcgcgag gcgggagatt tcgcggcggt ttcgccggga gcgtctttcg
    13861 atccttcctt aggtcccagc tcgtcgaaca cggtcatcag gaacactgcg gtagggacgg
    13921 acaggagaag gccggcgaac ccgagcagct tgccgagaaa gaggaaggag aggatcacga
    13981 ccaccgggtg cagccgggcc tcgtgcccca tcaccatcgg ctggatcacg tagccgtcta
    14041 tctggttcag gatcacaaac agcaccacca cgaggagcgc tttgaggggc gagatggaca
    14101 gggcgagcag cgccgggagg agcgcgccga ctatcgagcc gatgtagggg attacgttga
    14161 gcagccctgc caggagcgcg aacgtgatgg gcagcggagc gcctacgatc cacagtccga
    14221 gcccggcgcc cgtcgccagg aaaatcatcg caagacccgt accgactatc cagccgcgca
    14281 acctcttttc gagggcgtgc atgagcctcc ttgtcgcctc gtggtgcccc ggcggcacga
    14341 gcttgaggac gcccgagatc agaactccgg gatcgtaggc catgaacacg gccaggatga
    14401 acgtcgccag cgccgaggtc gcggtgtggc cgacctccgc cagcagcctc ggcagcgatt
    14461 cgatgccccg ttcggcgcga ccctttatct ggtcggccac gtgcgagagg tccgggacga
    14521 aaccgacgtc ttcgttaaga cgccgggcgg cgtgtgtcag cgagtccgcg taccccggca
    14581 tcgccgccgc cagcgcggag acctccgcca ggatcctcgg cgccaccacc aaccagacga
    14641 gcgacccgaa cgtcgcgaac gagatcaaaa gcaccgccac ggacatccac ggtctcaggc
    14701 cggcctcgtg gagccgattt gcgacggtcc ttattaccag ggcgagaagc atcgctaccg
    14761 ccaccagctc caggaccggc agcaactcgt aggcgatcaa gagcccagcc gcgagcagca
    14821 ccgccagggg cgccccggtg gttagcgttt cggatcctct cttttctctg ccgggtgcgg
    14881 gcataaatcc cctccagagt accgaatcgc acttccccct atttctactc ccgttcgcct
    14941 ccgggtcgcg gcgccgtctc ccgaacgccg gcaaacctta acgacacctt taccgaacgg
    15001 ctgctggcgg acgccgaacg ctaaccgcgc cgggcaacaa tcgccgtgga cgcgaccggg
    15061 gatgccggag gggatgccgg aaacctgcgg accccgggtt cgtatgggag tggagtacca
    15121 tacccgttgg cgagcggagt acagagagga gaggatgcga tgccgcgaga aggcgacgaa
    15181 cccctggtcc ggctggcgaa gacgatcctg gacttcaact ggaccggcga gtacacgaaa
    15241 cccggcccgc gcctctaccc gcatcagtgg tcctgggact ccgcgctcat cgccatggga
    15301 tacgcgcact acgaccagga acgggccgag agcgagctaa agcacctctt cgaggcccag
    15361 tgggagaacg gcttgctgcc ccagatcgtc ttcaacccgc acttccacga ctactttccg
    15421 gggccgggct tctggcacgc caaggagagc ccccacgccc cgaagcaccg caagacctcc
    15481 ggcgtggtgc taccgcccgt ccacgccacc gccgtgctgc gcgtctacca gcgcgccgaa
    15541 gacgggagag gagcgaagaa gttccttgca tacgccttcc cgaaactgaa cgcctggcac
    15601 gagtacctct accgcgagag ggacccggaa gacgaagggc tcgtctacat ccgccacccc
    15661 tgggagtctg ggatggacaa ctcgccgatg tgggacgcga tcatgcagcg gatgcagctc
    15721 cgggccggcc agataccaga gtaccggcgc gcggacaccc acaccgtcag cgcgcaggac
    15781 cggcccacca gcgcctccta cgaccgcttc gcctacctgg tgaagttctt cgccgcccgc
    15841 aattacgacg aggcggagat ccgggccgac tgcccattcc tggtccagga cgtgctcttc
    15901 aactccctgc tgtgccgggc gggccgggac ctggcggaga tagcgcgcat cctggacgag
    15961 gaccccacgc cctttgagga gagtgccaag aagacggccc gcgccgttaa cgagaagctg
    16021 tgggacgagg agcacggcat ctacctcgac tacgacctcg tctcgggctg tcccatccgc
    16081 gtctacttcg cgcccaactt ctcgccgctg tacgccggca ttcccagtga ggagcgggcg
    16141 aagcgtatgc tcgactctat ggagaacgcc ggcttcggcc tctccgacga ggacataacc
    16201 ccggtgccga gctacgacat acgcggcttc gggttttcac cagtccagta ctggcggggt
    16261 ccggtctgga tcaacattaa ctggttcctg atgcacggcc tgagacgcta tggcttcgag
    16321 gagcacgccg agcgcttgcg caagacaata atagacttgt gcgggaccga ggggttttac
    16381 gagtacttcg acccgacgac cggccagggg cacggctcca acctgttctc ctggtcggcg
    16441 gcgctgctcg tggacgtact gatggacgag aggagctgag ccatgcggct ggagaacaag
    16501 gtaaccgtcg tcacggggtc ttcgctgggt atagggcggg ccatcgcgct cgccttcgcc
    16561 cgcgagggcg cctcggtcac cgtggattac aggaagcact ccgatgaggc cgaagccgtg
    16621 gtcaaagaag tagaagagct tggtgggcgc gccctggcgg tgcaggcgga cgtctccgtc
    16681 ccggaggaca tccggcgcct cgtccagagc gccgtggacg agttcggccg cctcgacgtg
    16741 atggtcaaca acgccggcat agaggagaag atgccgttcc tggagacgcc gctcgacgtg
    16801 tggaaccgga tattatcggt caacctcacg ggggcctggc tcggctgcca ggaggcggcg
    16861 aagcagatgg tcgccggggg cgggggaggg cggatcataa acgtctcctc ggtacacgag
    16921 gaccggccca tgccgacgaa cgctccctac tgcgcggcca agggcggcgt gcggatgctg
    16981 atgcggacca ttgccgtgga gctggccccg cacggcatca ccgtcaacaa catcgccccc
    17041 ggcgccatag agaccccgat caacgagaac ctggacgaac acccggagca gaaggaagag
    17101 ctgctctctg agataccgct cggccggata ggcaagccgg aggaggtagc gaacctggcc
    17161 ctgtacctgg cctccgacga ggcctcatac gttacgggca gcaccttcac catcgacggg
    17221 ggcatgatgc gccacgcggg gagtctgtga agcgtcgctt cggcgtcagc cagttcacga
    17281 ccaccccctg gagcttcgag caggacgtcg aaagctacgc ccggctcggt gttgacgcca
    17341 tcgaggtctg cgaggacaag ctcgacggtg aacgtttcgc cgaacagctg gccctgatcg
    17401 gcgatcacga tatggagata agctccgtcc agcccgccgt ccgcacgctc ttccccagcc
    17461 gcacgcagcc ggagccgaag gacgtccccg ggcggctggc actctaccgg cgcgccatcg
    17521 agcggatcgt cccgttcgcg cccggctccc cgttcgtctg caacaccgga cctccgccgg
    17581 acggaaatat acaggaggta ctcgatacgg ctgtgaagga gtaccggggg ctcgcggact
    17641 tcgccgcgga acacggagcg cgcatagctc ttgagccgtt gagcccggcg ctgatgaacg
    17701 tggagagtgc gatctggacg ctggagcagg ccatgcgggt catcgccgcg gtggaccaag
    17761 aaaacttcgg cgtctgcgtg gacacctgga acgtctggca gacccccgac gtagtagaag
    17821 cgatagaagc ctgcggggat cgtatcttcg tcgtgcacgt tagcgactgg cggacgccgc
    17881 gatccttcgc cgaccggcac gtcatcggag aaggcgacat cccactgcca gcattgctcc
    17941 gggcgataca ccggagcggt taccggggag cgcacacact ggagatattc tccgaaggtg
    18001 tgccggactc gttgtgggag agggacctgc ccaccataat aacggagagc cgggccgccc
    18061 tggatgaagc gtggcgacag gcggatgaac caggataaga accgggataa cgaacgcgcg
    18121 aacggagccg tcgctgccgg ggcatgttct gcccgaccgc gtcccgcgaa cggggttacc
    18181 ctcgaccgac tccatcggcc ggacgcacct cgaaacagca gcgggggtct tccccgcgct
    18241 cgcagcgctc gcggacccgg acagccgcca actccgcgat aagggcctcc accagcccgc
    18301 acacctgtgg atgctgctct gccagcgccg ccagcggaca cccgtagcca tggatgacga
    18361 agacaccttc gttttcttcg atctcggcga accctcccaa accatcgaga accctcacga
    18421 ccgcccgcag gcgctcccga acgtctcccg aaggactctc gtgcccggcc gccatgcgag
    18481 cgcccgtctc ctttagcagc gcgaccgttt cctcggaccc ggcgcgctct gccagaatat
    18541 ccaaaagccc gccaaggacc ggctcgtagc cctttgggaa caacccccgc gcctcgggcg
    18601 tcagctcata cgcatgagcc ggcttgccgc tcccgccgcg acgcacgtaa ccaccatgac
    18661 gcacgagacc gtcgcgctcc agtgtagcga ggtgcgcccg aaccccgttg cccgtcagtc
    18721 ccagttcgga cgccaactcg tcgaccgtac gccccgtacg cctgagcatc gtgacgatcc
    18781 gcccgcgcgt gctctcgaaa aacttacggt cccaatgagc catcaacagt tatcccttct
    18841 ctgtagcccc tgcactgtaa cagggggaga taaattattc aaataaataa agtagtttat
    18901 tgatttattc ttgatgcggg tgtatcgtgt ggaggaaaga ggccgggttg gccggggaaa
    18961 ggagtagtgg ttgtggagag tagagccagg atgatgggac atccgataca cccgatcttg
    19021 atcccgttcc cgctgggatt gcttacgaca tcggtcgtct tcgacatcgt gcacctgatc
    19081 acggggtcgg ggcagtgggc ggtgatctcg ttctggatga tcgccgctgg cgttatcggg
    19141 gggcttgcgg ctgctgtgtt cggcctgatc gactggctgg ggataccatc cggcacgcgc
    19201 gcgaagtccg tcggcctgtt gcacggcgcg agcaacttcg tcatggtggc gctgttcgcc
    19261 gtaagctggc tgctccggct ggaagcgccg ggagcgccgg ggatcgttgc gatcgccctt
    19321 tccttcctgg gcgtcggact ggccgcgctc ggaggtttcc tcggtggcga gctggtcgtc
    19381 cggatgggag tgggggtgtc cgaggacgca aacctgaacg ccccgagctc actctccggc
    19441 cgtccggcgg gtgcgggatc cagcacagcc cgcagggtgc aataggaggc tggcgagatc
    19501 cccgacgagc agcgcgggga gacagagtct cttcgagccc tcgtggctac ccaagggacc
    19561 tgctgatgtt cgctgaccga acggagacca gatgatacat gggaccgaaa cccggatacc
    19621 agcgaaacct ttgccaaaag aggcttctga agcagagggg gatctcgccg tgcgggcgga
    19681 ggaggtgctg cgagataacg acatgggtgg ctggacccgc cccgcatccg gtctctatcc
    19741 tcaccagtgg agttgggatt cggcgttcat agccatagga ctcgcccacc tcgatgttcg
    19801 ccgggccgcc cttgaactcc gcacgctctt cgcccaccag tggaagaccg gcaaggtccc
    19861 gcacatcgtc ttcaacccgg ccgccccgcc cgagagttac tatcccggag cggagcactg
    19921 ggcctgcgcc gcgtcctcac cggacgcccc gctagcgccg ccctacacta gttgcctgac
    19981 ccagccgccc gtgcacgcca tagccgtccg gcgcatctac gagtccgccg gtcgcgaggg
    20041 tgaagaatcc ctctcccacg cacgcgagtt cctgggagag atctaccccc ggctctttgc
    20101 ctggcaccgc tacctggcca cctaccggga cccggagcac tcggggctgg taaccatcta
    20161 ccacccctgg ggaagcggcg ccgacaactc gccgcgctgg gacgaaccgc tggcggccgt
    20221 ggaagtcggg aacctcccgc cctaccctcg atacgacctc cagcacgtgg gagacttctc
    20281 ccagagaccc accgccgccg actacgaccg ctacctctgg ctcgtcgggc tcataaagag
    20341 agccggctgt gacgagagga caatctaccg ttcccacccg tttctggtaa aggatgtcct
    20401 ctccagcgcg atcctggtcg ccgcaaacga ggcgcttctg gagatatccg aggtcgccgg
    20461 agccccggac gaggaccggg agatcattag cgaatggatc caacgtggtc gcaggggtct
    20521 cgatgcgtgc tgggatgagg acctggggct ctgcctggac tacgacctcc gggcagacag
    20581 gccgctgcgg gttcggaccg tggcgggcct ggccccgctc atagcgggcg aacagagccc
    20641 cgaacggctc gacaggctcg tcgcgacgct agactcgccg gctttcgccg gacacgcggg
    20701 gctgcgctgg cccgtgccgc cctccacgag ccccggcgag ccggggttcc gcccgcgcag
    20761 ctactggcgc ggaccgactt ggccggtcat gaactggttg ctgtggcggg cgctcctgct
    20821 cgccggcgag acccggcggg ccgagaccct gcggcgggcg tcactggagc aggtcgcggg
    20881 agaggggttc ggggagtact tcgagccgtt caccggtgaa ccactcggtt ccctgcggca
    20941 atcctggacc gccgccgtcg tcctcgactg gctggcgagt ggtccgtcgc gggagccgct
    21001 cgatgaggcg cggcggaagt gaccgggccg ggctccggcg ggggcgggtc gttctggatc
    21061 gagacgaccc cccgcacgaa ctttccggtg ttagatggcg acgtggcggt ggacgtcgcc
    21121 gtgatcggcg gcggaatcac cggcctgacc gccgcgctcc tgttgaagcg cctcgggaag
    21181 acggtcgccg tcatcgaggc ccgccgagtg gcggagggtg tttcgggcaa taccacggcg
    21241 aaagttacct cgcagcaccg cctgatctac gcctggctca tcgagcggca cggggagcgg
    21301 aaggcccggc tctacggcga ggcgaacgag gctgctatct cgctcatcgc atccttcgtg
    21361 aatgagaaga agatcgagtg cgacctcgtg cggctgccgg cctacgtgtt cgccgagacg
    21421 gacgcgatgg tgccgcagct ccgggccgag gcggacgccg cgcggcggct gggtctcccg
    21481 gcgggcttcg ttcgcgaggt gccgctgccg tttccggtaa agggggccct ccggttcgac
    21541 aaccagttac agttccacat caggaagtac ctgctgccgc tggccgagga tatacccggc
    21601 gcgggcagcc acgtcttcga ggggacgcgc gccttgaggg tacgcgaggg cctgcggtgc
    21661 ggggtggaga cggaccgggg gaccgtggcg gcgagggatg tcgtcatcgc cacccacgtc
    21721 ccgttcccgt tcaaggggca gttctggggc aaggcaatcc cgaggcggga gtacgggatc
    21781 gcggcccgca ccggctctac gcccgtatcc gaagggatgt tcatcagcgc ggagagcccg
    21841 atccggtcct tccgtaccgc tccgcgcgac gggcgcgagg ttctcatcgt cgtcggcgag
    21901 agccacaaga ccggcgagga gcccgacacg gagcggcgct acaaaaagct cgaagagtgg
    21961 gccacatcgc gcttcggggt gacggagttc gagaaccgct ggtccaccca ggactacttc
    22021 ccggtggacg gtctgccctt tgccggcacg ataggcgccg gctccgagca cgtctacgtc
    22081 gcgacggcgt tcagaggatg ggggctctcg aacgggactg cggcggcgat gctgatctcc
    22141 gacctgatct gcggcaggcg caacgagtgg gccggactct acgattcaaa ccgcaaaggt
    22201 cccctgatct ccgggagctt ctaccgggag gggatcgagg aggccgtgca cttcgtgagg
    22261 gaccgcctcc gggcctccgg ccgtagcccc gccgacctcg gaacgggcga ggggagggtc
    22321 gtccgggtgg gcggcgatac ggtggcggcc taccgggacg agcggggcga agtccacgcg
    22381 gtctccgcgg tctgctccca cctcgggtgc atcgtctcct ggaacccggc cgagaagagc
    22441 tgggactgcc cctgccacgg ctctcgtttc gacgtggagg ggaacgtcct ccagggaccg
    22501 gcgacccgga acctgccgcg caagaacgta ggcccgtagc cggagtccgc cagcgggggc
    22561 gctatcctcg aaaatattcg ctcccgcgag ccggtgcgga tcgcggcttc ccttaatcga
    22621 tctttacagc acctttaccg accattaacc gcagcttgcc gaaggctgac aaggccgggg
    22681 aagaatcata ggcgatggcc cgcatggcgc aagccgttcg agccgaagct gttgcgtaag
    22741 atccaaggag gaagagtctt gagcggtccc cgtcccgcga ccttgcggcg catatccgag
    22801 gagccacgat gagcccggag atcggcaacc gccgctgggc catagcggag ggctacatcc
    22861 ccggctggtc gaacggtccg gagccccagt tcacgagcca cgagaccgcg tgcctgctca
    22921 acacctcgga cgaggacgct cacgtagaga ttacggcgta cttcagtgac cgggagccgg
    22981 ccgggcctta ccgggtgaaa gttccggccc ggcgaacggc gcacgtgcgc ttcaacgagc
    23041 tttctgaccc ggaaccgata ccggccgaca ccgactacgc gagcgtcatt gagtcggacg
    23101 tgccgatagt ggtgcagcac acgcgcctgg attcgcgcca ggccgagaac gcgctgctct
    23161 ccaccgtagc ctacgcgagc cacgattagg gaggtctcta agatgcccaa cacagcagag
    23221 ttcatgctca accgcctcag cgagtggggg ataaagagga tctacggctt ccccggcgac
    23281 ggcatcaacg ggctgctggg cgcctttcac gaggtcggcg accgcatcga gttcacccag
    23341 acggcgcacg aggagatagc gtccttcgcc gcctgcgccc acgccaagtt caccggcgag
    23401 gtcggggtgt gcatggcgac ctcggggccc ggcgcgatcc acctcctcaa cggcctctac
    23461 gacgcgaagc tcgaccacca gccggtcgtc gccatagtcg gccagcagaa gaggatgggg
    23521 ctcgggggac actttcagca ggaggtggac ttgctcagcc tctacaagga cgtggcgaac
    23581 gagtacgtcc actactgcat ggtccctacg caggctgcgc acctgatcga ccgcgccatc
    23641 cgaatcgcca aatcgcgccg gaccgtaacc tgcatcatcg tcccggacga cgtggctgag
    23701 atggaccacc aggacccgcc gcgcacgcac ggcgccacct tctccggcga gggagccccc
    23761 aaccagccgc atgtcgtccc gcacgaagcg gacatacagc gcgcagccgg ggttctgaac
    23821 gagggccgga aggtagccat gctcatcggg gctggcgcct acggcgcggc cgaggaggtc
    23881 agggaggtcg cggacctgct cggatgcggg gtggcgaagg ccctgaacgg caaggccgcg
    23941 ctgcccgacg acctacccta cgtcaccggg tccatagggc tgctcggcac gaagccctcg
    24001 aacgacatga tcgagggctg cgacacgttc tttatggtcg gatccagctt cccctactcg
    24061 gagtggctgc ccgagccggg gcaggcgagg ggcgtccaga tcgacataga cccgaccctg
    24121 atcggcatcc gctacccgat ggaggtcaac ctcgtcggcg acgccaagga gaccctgcgc
    24181 gccctgatcc cgcacctcaa tcgcaaggag gaccgctcct ggcgcgaaga gatagaggag
    24241 aacgtctccc ggtggtggga gatcgtcgag aaccgggcga tggaggacgc cgacccggtg
    24301 aacccgcagc gcgtcttcca cgagttgagc ccccggctgc cggagggctg catcgtcacc
    24361 ggagactccg gctccgcgac caactggtgg gcacgccagc tcaagctccg ggacggcatg
    24421 atggccgcgc tctccggcaa cctggcgacc atgggaccgg gcgttcccta cgccatcgcg
    24481 gccaagttcg cccacccgga ccgtcccgtc atcgcctgcg tcggcgacgg cgcgatgcag
    24541 atgatcggca acaacgcgct catagacgta atgaagcact ggcggcgctg gagcgacccg
    24601 cgcctgatcg tgctcgtcct gaacaaccgg gacctgaacc aggtcacctg ggagcagcgc
    24661 gtcctggccg gcgacccgaa gctcgaagcc tcccagaacc tccccgactt ccccttcgcc
    24721 cgctacgcgg agttgctcgg cttcaagggc atccgggtcg acaatccgga cggcgtggca
    24781 ccggcctggg aagaggcgct cggtgccgac cgccccgtcg tctacgaggc cgtcaccgac
    24841 cccgagatac cgcctctgcc gccgcacatc cgcttcgagc aggcccaggg catggcctcc
    24901 gcgctctcta agggcgaccc gcacgagatc cggatcgcct ggaactcgct caagggcaag
    24961 ctacaagaga tcacgacccg ctaggatgaa catgggcgag aagattccgg tcgagagggt
    25021 agaggtctcc gcgttcacga tcccgaccga ttccccggag tcggacggta ccctctcctg
    25081 ggactcgacg acgatggtgc tggtcgaggc cgcgggcggc ggcaagcggg gcctcggcta
    25141 cacctacggc gacgtctccg taggaaagtt catagagtcc aagctcgcga gcgtcgcgca
    25201 gggcgccgac gccatgagcc cacccgcagt atgggccgcg atgcagcacg ccatccgcaa
    25261 cgccggccgt cccggcgtcg gcgcgatggc ggtggcggcg gtggacatcg cgctctggga
    25321 cctgaaggcc cgtatcctgg accttccgct cgcggacacg ctgccccggt ttcacgaccg
    25381 cgcgccgatc tacggcagcg gcgggttcac ctcgtactcg ctcgataggc tgcaagagca
    25441 gctcggcggc tgggcctccg agggcatacc ccgcgtcaag atgaaggtgg gacgcgagcc
    25501 ggagcgggac cccgagcggg tccgggccgc gctggaggcc gtgggcgacg gcgtcgagtt
    25561 gatggtggac gccaacggcg cctacacgcg caagcaggcc ctccgatggg ccgggcgctt
    25621 tgaggaggcc ggaatcccct acctcgaaga gcccgtctcg tcggaggacc gcgagggctt
    25681 gcgacttctg cgagaccgcg gacccggcgg tctcgccatc gccgccgggg agtacgaatg
    25741 gaccctgccg gatctcgccg acctggccgg ctgcgtggac gtcctccagg ccgacgtcac
    25801 ccgctgcggc ggtatcacca acctgctgcg agcggacggc atctgcaagg gacaccaggt
    25861 cccattctcc gcccactgcg cgccggcggt ctcggcccac gcctgctgcg cgatggagac
    25921 cctcttgcac ctcgaatact tccacgacca cgtgcgcctg gagaacatgc tcttcgacgg
    25981 gaccctccgg cccgagggcg gttacctccg ccccgacccc tcgcggccgg gcctggggct
    26041 ccaactcaag cgttccgaag ccgaactatt cgccgcctag agaatattca gaaaagaagg
    26101 agggagtaga tgacggacac cgttaagcgc gaaggctaca gcctcaacga ccgccaacgc
    26161 atgaacctca acgccctgtt gctgcagtcc gagctgcgcg acgcggtgaa gggcgaggtg
    26221 cgctttgacg ccggctaccg tgcggcctac tcgacggaca gctccaacta ccgccaggcg
    26281 ccgctcggcg tcgtggtgcc ccgagacccg gacgacgtgg tggccgcgct ggccgtctgc
    26341 cgccggcatc gggtgccggt aacgccgcgc ggctgcggca ccagcctggc tggccagggg
    26401 accaacgcgg cggtcgtctt cgacgtctcc cggtacatgc gcgagatcgt cgaggtggac
    26461 cccgagaaaa agatcgcgcg cgtgcagccc ggcgtgatcc gggaccaact cgccaagccg
    26521 ctggaggccg agcacgggct ttccttcccg cccgacacct ccacccacgc ctacgccacc
    26581 ttcggcggca tgatcggcaa caactcctgc ggcgcgcact ccgtcatgac cgggcgcacc
    26641 tccgacaacg tcgaggagat ggacgtcgtc ctctacgacg gcacccggat gcgggtgggc
    26701 gccacaagcg aggaggaact ggagcggatc attcaagagg gtggacgtaa aggtgagatc
    26761 tacgcccgac tgcgcgacct gcgcgaccgc tacggcgacc ggatccggga gcgctacccg
    26821 cagataccgc gccgggtctc cggctacaac ctggacgaac tactgcccga gaagggcttc
    26881 aacgtcgccc gcgcgctggt cggcaccgag gggacctgcg cgttcgtgct ggaggcgacc
    26941 gtcaggctga caccctggcc gccggcgcgc tcgatcctgg tgctcggcta tccctccatc
    27001 ttcgaggccg ccgaccacgt gccggaggta atgtcccacg ccccgatgtc ctgcgaggcc
    27061 atagaccggg agctaataga cgacatgctc cgccagggta tgcacgagag cgaggtcccg
    27121 ctgctccccg acggggacgg ctggctgata gtcgagttcg gcgctaagac gcgcgaggag
    27181 agcgacgccc gcgcccgcga ggtcatggag gctctggagc gcgacgagac cacgccccgc
    27241 atgcgcctct tcgacgacga gaccgaggag gcgaagctct ggagcgtgcg cgagtccggg
    27301 ctcggcgcca ccgcgtacta tcccggcggg gccgaccact acgagggttg ggaggacacc
    27361 gcggtgccgc ccgagaagtt cgggcattac ctgcgcgact ttcaggacct gctcgaccgc
    27421 tatggctaca acacggccat gtatggccac ttcgggcagg gcctcgtcca ctgccgcatc
    27481 aacttcgacc tgaagagcgc gagaggcatc cggcactggc gggagttcct ggacgaggcc
    27541 gcagacctcg tcgtccgcta cggtggatcg ctctccggcg agcacggcga cgggcagtcg
    27601 cgcgccgaac tgctcggaaa gatgtacggt cccgagttgt tgcgggcctt ccgcgaattc
    27661 aaggccatct gggacccgga aaacaagatg aacccccaca agatcgtcga gccctaccgg
    27721 atcaccgaaa acctcaagct cggcgccgac tacgatcctc cgaacgtaga tacgcacttc
    27781 gcctaccctc aggacggcgg cagcttcgcc cacgcggccg tgcgctgcgt cggggccggc
    27841 aagtgccgcg acacctcgac cggcacgatg tgccccagct acatggtcac gctcgacgag
    27901 gagtacacca cgcgcggccg ggcccgcgtc ctcttcgaga tgctgcgggg cgagaccata
    27961 aaggacggct tcaagagtga cgaggtcttc gactccctcg acctctgcct ctcctgcaag
    28021 ggctgcaagg gtgactgccc cgtcaacgtg gacatggcta cctacaaggc cgagttcctc
    28081 tccaagtact atgagagcag gatcaggcct cgctacgcct atacgatggg cctcatcatg
    28141 ttccacgccc gtctggctca gtacgtcccg cgcctggcga acttcgtcac ccacgccccc
    28201 gtgctcaagg acgtcgttcg gaaggccggc ggcatcacca ccgagcgcga gatgccgccg
    28261 ttcgcaacgc agaccttcaa gacgtggttc gaagagcgcg gcggcgcggc gaaccccgcc
    28321 ggcccgccgg tcgtgctctt tccggacacc ttcaacaact tcctccaccc cgagacggcg
    28381 aaggccgcca ccgaggtgct ggaggccgcc ggctaccagg tgatcgtgcc gcagcagttc
    28441 ctgtgctgcg gacgtccgct cttcgactac ggcatgctcg acaccgcgaa ggtctttttc
    28501 gaccgcctga tagacggcct gcggccctac gtccgggagg gcgtccccat agtcggcctc
    28561 gaaccgagct gcgtggcggc cttccgggat gagcttccca acatgatgcc caacgacgaa
    28621 gacgccagga ggctcagccg cgacatcctg accctgagcg agttcctggt ccaggaggca
    28681 aagcactacg atcctcccaa gctgaaccgc aaggccatcg tgcacggcca ttgccatcag
    28741 aaggcgatca tgggcatgag cgccgagcag caactctacg agaagatggg gctcgactac
    28801 gaggtcctcg actccggctg ctgcgggctc gcggggtcct ggggcttcga ggaggacaag
    28861 tacgacctct cgatgaagat cggggcgagg aggctgttcc ccgccgcccg cgacgccgat
    28921 acggacaccc tcgtcatctc cgacggtttc tcgtgcaaga cccagctcca gcaggggacc
    28981 gaccgccgcg ccctgcacac ggcccaggtc gtgaagatgg ctctcgacca cggcgccggc
    29041 ggcgctcccc gcggcgagcg gccggagtcc gggtacccgg atgtgattct cgacggtgcg
    29101 cgtcccgacc tgaagaccgc cgccgtggct ggcgcggcgc tcgccctcgg ggcggcggcg
    29161 ctctgggtct tcaggggaag gaggtagtcc cctgcgtaac ctctggacgt acgaatacgg
    29221 gacgtctaca gggtttttag gtggcgggtg aatacctttc tcaggataac caggatagga
    29281 gcgctggtct ccttctaccg acgtgcgaga acccggaatg tccggccccc cggacgggca
    29341 tcaagctcca gggcgacggg ttccttcacg aagattgtta aagtcctacg gtaaaggaga
    29401 tctggcatga gcaacggaac cggcaacgga agcgaagtgg tggtaatcac gggagcctct
    29461 gccggtgtgg gccgggcggc cgtccgggcg ttcgcggagc ggggcgcgcg catggggctc
    29521 ctcgcccgca acccggaagg gctcgaaggc gcccgccgcg aggtggagga ggccggaggc
    29581 aaggccatag ccatcccgac cgacgtggcc gaccacgagc aggtcgaggc cgccgcacaa
    29641 aggatagagg aggagttagg accgatagac atatgggtga acgacgcgat gaccacggtg
    29701 ttcgccccgt tcaagaagat ctccgccgcc gactacaagc gggccacgga ggtcacctac
    29761 ctcggcgcgg tctacggcac gatggcggcg ctcaagcgca tgctgccgcg tgacaggggc
    29821 accatcgtgc aggtcggctc ggcgctcgcg taccgggcga taccgcttca ggcgccctac
    29881 tgcggggcga agcacgcgat ccggggcttc accgactcga tccggaccga gcttatccac
    29941 gacaggagca acgtccgaat cacgatggtg caacttccgg gcctgaacac cccgcagttc
    30001 gaccactgca agaccgagct tccgaaccac ccacagccgg tacccccgat ctaccagccc
    30061 gagatagccg ccgacgcgat agtgtgggcc gcccaccaca agcgccgcga ggtctacgtg
    30121 ggcgcgtcct cggccatcac gatcgtcggc aacaagttct tcccgcggct cggggacgtc
    30181 tacctcggga agaccggcta cagtggccag cagacgagcg agcccgtcgg ccccgacagg
    30241 ccgtcgaacc tctacgagac cgtccccgga gaccacggcg cccacggcat cttcgacgac
    30301 caagcgcacg aaaagagccc gcagctctgg gcaaccaaga accggcgctg gatcgcgctc
    30361 acaggggccg gagccctggc agccgcgacg ctcttcttgc ggaggtagac ggatgcatga
    30421 acgcgcgacc ccgcccggct tcgacaagaa ccccacgaga tggtcgaggc gattgctcct
    30481 ggccgcgctg gccctgctcg ggctcttcgt atccgcgtac ctggcgctct accagatcgg
    30541 ggtgatcggc ggcgtgtggg acccgttctt cgagagccgc agggtgctcg acttcctggg
    30601 cgtcccggac gccgcgctgg gggtcctggc ctacgccacg gagataatcc tgctgttcat
    30661 cggcgggcgc gagaggtggc gcaccatgcc gtggacggtt ctggccctcg gcgtcgtgat
    30721 cctctcgggc gccgtcgtta gcgtcctgct gatactgatg caggcgtttc tggtcggcgc
    30781 ctggtgcacg ctctgtttgg cctccgccgc gatctccctg gcgatcttcc tcctcggcgc
    30841 cgacgagcct gtggccgggc ctcgctacct gaaacgggtt cgcggctcgg gcggctccgc
    30901 ctggcgggcc ctgtggggga gaggagactc ccgcgaaaaa aggaggcgct cgcatgcctg
    30961 agagtccgcc ttacccgccc atcagggact acgcgctgat cgccgacagc aactcggtgg
    31021 ccctcgtctc ccgcgccggc tcgatagact ggtgctgcat ccagcgcatc gacgcgggaa
    31081 gctgcttcgg gcggctcctg gactggcagg agggcggctt ctgctccatc tctcccgcgg
    31141 caggctcggg cgacgtctcc cggcgctacc tggacgagac gctggtcctg gagacgacgt
    31201 tccgcgtcgg gggaggcgtg gcccggctct acgacttcta cgcgctcccc gccagcaccg
    31261 aagagcaccc gtaccggcag ttgatccgga tcgtggaggg tgtgagcggg agcgtcgagc
    31321 tagatcttga gataatgccc cgcttcgact acggctcgat gaagccctgg atccgccagg
    31381 aaggcgtccg cgtctacagc gccatcgggg gcaacgacgg gctcttgatc tccagcgacg
    31441 cggacatagc gcagaccgac gaacacgccc taagagccgc ggctaccgtc cgcgccgggg
    31501 agcgggtgcg gctctccttc acctccgtgc cgccggagca actcgactac gaccggccgg
    31561 aagccctggg tgcgaacgag atggatcggc gtcttgagga gacgatagcc tggtggcgcg
    31621 actggtcctc cgcgatccgc ttcgaggggc agcgcaagtc gagtgtcctg cgctccgcca
    31681 ttaccgtaaa ggccctgatg aacgacctga cgggggccat cgcggcggcg gccaccacct
    31741 ccctgcccga gaaccccggc ggaagcctga actgggatta ccgctacagc tggatccggg
    31801 actccttctt ctccgtgcgc tcgctcgccg aggtcggttt cgatcccgtc gccgaccgct
    31861 tccggcgttt cgtcgagcgc agctccgccg gcagcgcgga tcaactccag ctcatgtacg
    31921 gtatgggcgg cgagcggcgc ctgaccgagg agacgctgga ttacctggag ggctaccggg
    31981 gcgcgagacc cgtgcgggtc ggcaacgccg ccgccgggca gctacagctc gacatgtacg
    32041 gggagcttct ggagcttagc tggcgctggc accagcgggg caactccccc gacgacgact
    32101 actggcgctt cctgatggag ctggtagaca ccgccgccga acgctggccg gagcccgaca
    32161 gcggcatatg ggagaagcgc ggagaccagc ggcacttcgt ccactcgaaa gtcatgtgct
    32221 gggccacgct ggataagggg ctgcgcctgg cgaaagagtg catgaggaag gcccccgaac
    32281 ggcgctggaa aaagacgcgg ggcgagatcc gggacgccat cgagagcgag ggctacgata
    32341 aacagcgcgg cgtcttcgta caatccttcg gtgctaaaga cctcgacgcg gcgctcttgc
    32401 tcctgccgag ggtggggttc gtggactaca aagacgagcg catggtccgc acgacggatg
    32461 cgatccgcga cgaactcgat gacgatgggt tgctcaaacg ctacaggata gacgatgggg
    32521 agcaggaagg agccttcctc gcctgctctt tctggctcgt ggagtgcctc gcccaccagg
    32581 gacgcgtaga ggacgcccga gccgtcttcg atcggaccct ggctaccggc aacgacctcg
    32641 gcctcttcgc cgaggagtac gataccgtca atggagagct acttggcaac ttcccccagg
    32701 ggctcacgca cctctcgcac atagccgccg ccgtcgccct ccagaagcac gaaagcacgg
    32761 tctgacgttg cttacgtcgc aaacccgtct gggcgggatc tcctgatgta cggcgggaaa
    32821 cgtggtggac tgcgcgggtg gctcgtggat cgtctcggtg gagggggaga gtcgatctac
    32881 acgttcttcg cctgcgtcct ggcaatggcg gtgagcgggt tcggggccta tctcacgaaa
    32941 cagcctctgc tgttcccgag cctcgggccg acggctttcc tgttcttcga gtcgccgatg
    33001 tcggcgcagt ccagcccgcg caacaccctg atcgggcacg cggtcgcggt cctcgccgga
    33061 gccgcttcgc tcgccgtatt cggactgctc caccacccga gtgtgctcgt ggagggcgtc
    33121 agcccggccc gcgtcggggc cggggcgctc tcgctcgcgc tgacgggatc cgtcttgctc
    33181 cttctgaagt cctcgcaccc gccgacgggg gcgaccacgc tcatcgtgag cctcggcctc
    33241 ctgaagacgc cgcgccagat ggccgtgctg atggccggcg tcgtgttgct gacggtggtc
    33301 tgctggatcg tcaaccgcgc cgccggtgtg cccgtgcctc cgtggtcgtc cagagagtag
    33361 ggcgtggccg tcgctctacg gcccggagaa aacgggactg tgtcagacgt tggcgaactt
    33421 gtatccggcg ccccgcacgg tctggatgta gcggggcttc gtggtgtcgg gctctatctt
    33481 cttgcggatg tgctggatgt gcacgtccgc agccctcgcc tcgccgaaga actcgccatt
    33541 ccagaggtgg cgcatgatct gctcccggct gtacacccgt cccggatggg cggccagcag
    33601 ggccagaacg tcgaactcct tggcggtcag gtccacctcg tccccgcgaa cgaagacctg
    33661 ccggccgaag aggtctatct tgagattcgg gaactccagc gtgctgcgct ccgccgggac
    33721 ggaagccgag tccaggcggc gcctgcggag gtgcgctctc acccggctga ccagctccct
    33781 gggcctgaac ggcttggtga cgtagtcgtc ggcgcccacc tccaaaccga ccacgacgct
    33841 tgtctcgtcg tcgcgggcgg tcagcatgat caccggcgcg ttcgccgagg gggagccgcc
    33901 cttcctgagc tcctggcaca cggagatgcc gtccatctcc ggcagcatga tgtccaggat
    33961 gacgacgtcg aaggggctcg ccgaggagaa acgctccatc gcctcctctc cgctggcgac
    34021 ggcttcgatc tccatgccct cgcggccgag cgcgtgcccg accacatccc tgaccgcggc
    34081 gtcgtcctcc accacgagca ccctagccat tttggtcctc cgtcgcggct tgcgttgcgg
    34141 cttgaggccc ggttcgcgcc ctgatctccg gcagctttat ctcgatcgtg gtcccctttc
    34201 ccttccgtga gctcagggag atctccccgt tcatgctctc caccagttcc ttgcagatcg
    34261 gcagccccag gccgaagccc tcggagtctt taccccggtg gaatctctcg aagacgtgcg
    34321 ggatgtcttc ttcttcgatc cccaccccct cgtcctctac ggtgacgacc ggtccgtgcg
    34381 tacgcagccg cacgcggcct cctctctccg agtgctgcac ggcgttgctc gtgagaacca
    34441 gcagcgcctg ctccagccat tcctcgtcag cccggacccg ccctccctca ccctcgacgg
    34501 tcagggtcag gcccgcgccc ctggcgagcg gccgcatgcg ttccgcgacc ctgccggcga
    34561 ccgtcccgag gtccacctcc ctcaagttcg gggccctgcg gtcccagccg gtccgggcga
    34621 gccggagcag cgtctccgag aggcgttgca tccgctccgc ctccgagcgg atgtgcttga
    34681 ggaaacgttc gcgcacgcgg ggatcgtcct cttcctctgt aaggagcagg tccgaggcgc
    34741 cgagtatggt ggtgatcggg gtcctcaact cgtgggcggc gttcgcgagg aagcgctgct
    34801 ggttcgcctc cagccgccgg ctctcggaga ggtcccggat taccacgagt actccgcccc
    34861 ggtgctcgtc gaaggccggc atgtgttcca ggttgatatg gaagaacgtc tcatccccct
    34921 gcgcccgagc ctcgccgcac tcctgccgcc tcgcgcagcg ggccaccgcg tcgggcaggt
    34981 cgaagtcccc gaacgggtcc ggcagttcct cgggttgccg gacatcctgc accctctccg
    35041 acgtcatgcc cagcatccta cgggccgaga ggttggcgaa catgaccctt cctttgaggt
    35101 ccgtggcgag cacgccctcg ttgaggttgt cgaggatggc gtccagcgtc gcgttgttct
    35161 cctcgagacg gctcatggac ttctggacct tggcggtcat ggcgttgaag gtctcggcca
    35221 gctcccccag ctcgtcgccg tagcccggct ctatgcgcga ggagaaatcg cctcgctcta
    35281 tcgccttcgc gcccagggtg aggcgctcga cccgccggct gaggagcgtc gcgaccagca
    35341 gcatcagccc gcccccgagc accgcggcga tcgccgccgc ctccacgttg ctgcgggaga
    35401 agatgtccag cacggcagat tcgctgttgt ctgagacgaa gacgagcccg ccccggaggc
    35461 cgccgccccg gaccaccggc acggtggtta cgcggtggtc ttcgatccgc tcgttgaggc
    35521 gattgcccct ggccgctgtg tcgattactc gctgcggaag cgcggatggc aggccactcc
    35581 cctcgcgggc cagcaccttt ccctttcgat ctaccagcag ggcttcgccg cccgaggact
    35641 tgaccgcgag cttcagcgcg tctccggcgc cgtccggact cgcgctctcg accgccgtcg
    35701 cggccgcggc cgcctcggcc agcgcgtgct cgcgcgagta ttctccaagg cgggtcttca
    35761 cagcctgcag accatagaga agtatcgtgc caatggcgag catgacgata agcacggcta
    35821 ccgccggcat ccatatccga aacctgagcc ccacgagaaa tattctatac gctccgcggc
    35881 agtcactatg tctgcgcctc ggtgaagcag agtctttcac gatgcgaggg ggacctctta
    35941 accgaacctt taccgaaggc ttaccggcat cttgcaagcg gttaaggccg ttcagggatg
    36001 atattcaagt ggaggcgtgc gctagagcgg aggtccatga ggagcggcgg tagcatacgc
    36061 tggcggggcg tgggtgccgg cgcgctggcg gcggccctga cggtctttct gctgagcccg
    36121 atggtgcggg ggctgtatga catcctcacg ggctctctgg cgccgatggg gaagttgacc
    36181 tccgggctga tcgtggtcgc cctcgtctcg ggattcctcg cctacctctt cggaggtttc
    36241 gttgccggca ggctcgccag gggcgcgggg ggcgcaaacg gcgcgatgac ggcggtgatg
    36301 gggaccgcgg tcggcgcgtt gctcggagcc gtggggatcg ccgtctccgg caatgtgtgg
    36361 gtggcgatca tgaacctcgg gtctgccggg ctggcggtcc tgatcggcct gctcctgctc
    36421 cttatagacc tctttggagg ttatatcggc ggcaagctcg gcgagccctc ggagcctcgt
    36481 atgcatcgtc ttcacgatta gcggggacgc ggactcccgg ttctcgcggc gcgctcgccc
    36541 ggagaagcgc gccgcgttaa catgatggaa tgcagatcgg cactcacacc ttcctcccga
    36601 ccgagcgcgt gcatttcggc gccgggagcc tgcggaagat agaggaggag accgggcgga
    36661 agggccgggc ctttatcgta accgggcgca gcctgaacga gaagacggac ctcatccggc
    36721 gcgtcgagga gatgctaggc gggagacacg ccgggactta cgcggcgatc tcccagcaca
    36781 cccccggcag cgccgtggag gaggcggcat ccgaagccga gatggcgggg gcggacctcc
    36841 tgatcagcat cggaggcggc agcgtgatag acggcacgaa ggccatctcc cgccagcttg
    36901 gctatccttc tcaggtagcc gtgcccacga ccctctccgc cgccgagtgg gcgcaccgcg
    36961 tcggcgtcac ggacgaggcc gccggtaaga agagaggctt cgccgacccg aagaccgtgc
    37021 cgcccgtcgt gatcctcgac cccgagacga cggtatacac ccccgaaaga ctctggctct
    37081 caaccggcat ccgggcgctg gaccacgccg tggaagggtt cctttacggc gggccgcatc
    37141 ccgtcacgga cgtaaccggc ctcgaaggag cccgtcgcct gatggagtac ctgccgatct
    37201 cgaagaggga gccggaggac ctggaggcgc gggcggagtt gcaggtcgcg gcctggctat
    37261 cgtacttcgg gccggcgaac acgccgatgg gactctcgca cgagctggga cgcaggatcg
    37321 gggcgagcta cggcgtggcg cacggcataa catcctgcat caccctcgcg ccgagcctcg
    37381 aagtcgtcag ggagcgcatc ccgaaggatc ggtgggcgcg gctcggcgag gcgctcggcg
    37441 gcgagccggc ggagcgggtc gcggatctcg tccgggagct cgggctgcca ggacgtctgc
    37501 gcgacgtgga cgtcccgcga ggggagatcg agcggatcgc gagcgagtac ggggagcgag
    37561 aggcggatgc ccgcgagatc ctcacccgcg cctggtagga gcgggtttca gccgccgatg
    37621 cgggactctt cgaggtccag gtcgtgctgg atgcggcgca tgatctccgg gcttatggct
    37681 ccggcgtcgc gcaggccgac tatcgcctcg cgttcggcgg cgattagctg gcggcgccag
    37741 ttacgccagg ccgacgagtc ctggttgtac tcctcggtcg ctccgccggc ctcgatgccc
    37801 gccgtgtagc gccggatgcg ctgctcgtag gactcgcgca tctgctgctg gctgtgcggc
    37861 gggacccgtt cgacggcgca cagcaggtcc agcttctcca gggcggcgcg ggcggcgtgg
    37921 agccgggcct ccagctccat gatcttgcta tcctctccgt cgcctttcag gcgcagagac
    37981 tttatcagcg gcggcagggt gaagccctga aggacgaggg tcgcaagaat aacgccgtag
    38041 gttaggaaca ggatctggtc gcgccccgga aacggcgcgc cggactgtac cgtcagcggc
    38101 acggcgaggg ccgccgccag cgagaccgcc ccgcgcatgc cgctccagct catcaccagc
    38161 cgctcctgcc agggcgctct caggtaggtg ctgtgcagga gacggtcgaa gacggggtgc
    38221 aggtagggcg tggtgaagaa ccagacgagg cgcgtgaggg cgaccgccgc gtacacgagc
    38281 ccggcgtaga ggaggagagt tccccaggtg gagcccgcgt cgagaccttc gaggatggag
    38341 gtgaactgca ggccgaccag gataaagagc agcgactcca ggacgaacgt caggacgctc
    38401 cagaaagaga gggcctgcag gcgcgtggac gcgtcgggga agagcctggg ggcgtgccag
    38461 ccctggtaga gaccgtagga gactattgcc aggatgccgg agaggtgcag cacctcctcg
    38521 gccaggatgt agacgacgta gggcagcagc acggaggtca taatgagaat cagggcgtcc
    38581 ttcagccgcg cccacagcgg cagcatcaag cgggcgaaga cgaggccgac gagcaggccg
    38641 cccgcgctca ccacaacgaa gttcaggccc gcctgcgtcg cggagaaggc gccggagacg
    38701 accgccacga cggccacccg gtagacgacg agggccgagc cgtcgttgac caggctctcg
    38761 ccgccaacga ccgtgctcac ggtcgccgga acccccaggc ggcggaagat cgcctcggcc
    38821 gcaaccgggt ccgtgggcgc caggaccgcg ccgagcacga acgccgcggc ccacgggagg
    38881 ccgacgacga agtgcgccac cgccgcgact gccagcgtgg tcgccagcac cagcccaacg
    38941 gcgagcatcg cgatggggcg caggtgggcg cgcaagtcgc ggggcgaggc cgtgaaggcc
    39001 gccgagttca gcagcggcgg caggaagacc aggaagatca cctccggcgc cagctcgatc
    39061 ttcgggagcc ccggcgcgag cccgatcgcc agcccgccga ggaccaggaa gatggggtag
    39121 gggacgttcg ttatgcgcgc gagctgcgcg aggaccgcca cggcgccgag cagcaggatc
    39181 aggaactcga tgtcgctcat ccggccctca tatctccagc cgggactctt ccaggtccag
    39241 gtcgcgctcg atgcggtgca tggcgtcgtc gctgatctcg ccctcgtttc gcagccggac
    39301 gagggtgcgg cgctgggcac gcagcagctc gatcatgagc cgctggtagg ccgtggagcg
    39361 ctcctcgagg ctgtccgagt cgccgtcgaa gcgggcggcg aaccgtcccc ggcggtagtt
    39421 gtagagaccg cgcacgcgct ccgccgtctc ctcgcgcacc cactcctcct cgaccaactc
    39481 ctccagccgg tccagggcgg cctgggcgac ctcgatcctg ccagttacct cttcccgctg
    39541 cgtgctgccg tcatcttcga ggccgagacc ccggatcagg gccggcagcg agagcc
//
LOCUS       DASSMB010000039        11060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1530811, whole genome shotgun sequence.
ACCESSION   DASSMB010000039 DASSMB010000000
VERSION     DASSMB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 11060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11060
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1530811"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VFJ72_02835"
     CDS             complement(<1..1085)
                     /locus_tag="VFJ72_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HET7478434.1"
                     /translation="MSSDQDNQRRFETRAVHSGERRAKHEEAGANEPSAFYPVSTPIY
                     ASTTFSHPNIETTDRVLGGEEPGYSYARYDNPTVAAFERAIASLEGSPEARAFAFGSG
                     MAAMHAALAAAELTGGATVLAAEQLYGSTATLLVQVFGAAGVEVRFVNCYDLEAVEKK
                     VEELRPRAVVIESISNPLLRVADVPAIAEVTRAAGSALIVDNTFGTPYLQRSLDLGAD
                     IVVHSATKYLSGHGDITAGLVVTGAPYDGAVEQLRKLVGGVIGPFESWLAHRGLKTLP
                     LRMSRQCENAREIASRLASHPKITKVNHPSLKDHPDTEVARRVLADTGGLVSFELAAE
                     SREAAFRFLNSLELCVKAPSLGDVYTLA"
     gene            1135..1449
                     /locus_tag="VFJ72_02840"
     CDS             1135..1449
                     /locus_tag="VFJ72_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478435.1"
                     /translation="MEDRISYFERMLADNPNNPTGLMALANEYGKAGRDEDEAAVLQR
                     YVEAHEDEGNAYARLGEVLARLGREDEARSAYERGIGQSEKHGHSGMADDLRLALLRL
                     NE"
     gene            1639..4500
                     /locus_tag="VFJ72_02845"
     CDS             1639..4500
                     /locus_tag="VFJ72_02845"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HET7478436.1"
                     /translation="MANLSEREFFGPNLGYVLELYERYREDPGSVDESAREFFETWSP
                     PRPGAGIAGTNGQVVPEVDVEKAVGASKHVRHIRDFGHRAAQLDPLGSEPPGDPTLNP
                     EFYRISDEDLETLPELIIGGPIAERTSNAREAVEELRKLYCNTTGYDFGHVHDPDERF
                     WLREAVESGRFAKRMEGEEAKRLLQRITRVDAFEKFLHKTFLGQKRFSVEGNDMVVPM
                     LNRLIRSAADVGTPVVTLGMAHRGRLNVLAHVMDKSYSKIFGEFQQPDKGEQASAAGS
                     PGGAWVGDVKYHLGIRGFHLDDAEADEEVLINLAPNPSHLEHVNPVVEGMARAAQEER
                     SEAGPPRLDVEAALPIILHGDAAFPAEGVSAETLNLSRLPGYKTGGTIHIITNNQIGF
                     TTERQDARSTTYASDLAKGFEIPVVHVNADDPEACLAVTRMAHAYREEFDKDFMIDLV
                     GYRRFGHNEGDEPAYTQPLMYETIRNHPTVRELWAKELERRGVIEEGEADAMFEEIMA
                     DMAEVQKNPDEGLTEEDVFTDEPYTPLAEIPETAVPAERLAELNKALLERPEGFTPNS
                     KLERLMRKSRGELNGSDSSAGIDWAHAETLAFASLLEDGTPIRITGQDTERGTFSQRH
                     AVLYDAKTDERYVPLQNIPQAKSSFSIYNSPLSEIAVIGFEYGYSVSSEDTLVLWEAQ
                     YGDFANVGQPMIDQFIVSGQAKWGQTSGLVMLLPHGYEGQGPEHSSARLERYLQLAAN
                     ENVRIANLTTAAQYFHILRAQAALKNQKRPLIIMAPKSLLRHPMAASKLEDLSEDSFH
                     PVLDDAEARERAESVERLILCTGKIFTELAGSEYREEAVDTAIARIELLYPFPEERVR
                     DLLDGYPNLREILWVQEEPKNMGAWSFVEPRLRDIADELPILYVGKPGRPSPAQGSAK
                     FHKQEHAEIIRMAFESVNEDLDDEGSVDETQVRASAKSE"
     gene            4544..5881
                     /gene="odhB"
                     /locus_tag="VFJ72_02850"
     CDS             4544..5881
                     /gene="odhB"
                     /locus_tag="VFJ72_02850"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HET7478437.1"
                     /translation="MAVEIHVPELGESVADATVGKWLKSEGDAVKSGDPLVELETDKI
                     NFEVEAEQDGVLESIASKEGDTVNVGDVIGTIGEGEAAGDGQPEDSGEEEEGSEEQAQ
                     TEQPEAEAETEEDEAPEEEAGGEEANGHREMEDGAGRASPSVRKLAEEYGVQLAEVSG
                     SGSGGRITRDDVERLIREKGQGGGQGGGAEKTEQPERDGKAEKPAPASQNGRADLEER
                     VRMSRRRLTIAQRLVESQQTAAMLTTFNEVDMSAVMELRKRRKESFQERTGARLGFMS
                     FFAKAAIGALKAFPKVNAELQDNELVLKKYYDLGVAVSTDEGLVVPVLRDADRKSFAG
                     IEQGIADLATKARDGKLGLEDLRGGTFTITNGGIFGSLLSTPILTMPQVAILGMHKIQ
                     ERPMVVDGEITVRPMMYVALSYDHRVIDGAEAVGFLVRIKELIEDPESLLLEG"
     gene            5885..6826
                     /gene="meaB"
                     /locus_tag="VFJ72_02855"
     CDS             5885..6826
                     /gene="meaB"
                     /locus_tag="VFJ72_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET7478438.1"
                     /translation="MPDGGLVERLVSGDKRALARVISKIEREDEDAPGIIAEIYPRTG
                     DAVTIGFTGPPGVGKSSIIAKLIKLYRDEDKTVGVVSVDPSSPFSKGAILGDRIRLSD
                     HFLDPGVFIRSMGSRGHLGGLAGGSRLAALAMEAYGCDVVLYETVGVGQGEVEVASAA
                     DTVVLALQPGAGDAVQALKAGVMEIADVFCINKADHPQAKNAASEVRSILEIGQELDP
                     DPWFPPIVMTRGDTGEGVEELKETIQKHRSYLKESGKLEERRRAALREFVVSWATNRL
                     EKEMHERLDREDTQVMEQVYERSLDPISASERIFKEV"
     gene            6830..7609
                     /locus_tag="VFJ72_02860"
     CDS             6830..7609
                     /locus_tag="VFJ72_02860"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HET7478439.1"
                     /translation="MAPRRIVSLVPSLTEALFAFGAGDRIVGRTRYCTQPPRAVGRIP
                     KVGGTKKVDVARVLQLEPDLVVAVREENTRGDVEALMQADVPVFLGSPATIAEAVKML
                     RELAGRVEAPLAGEVLEPIERTLGRLRDSAKETRRVFVPIWKHPYMSVGRDTYVHDVL
                     KTCGGENVCGGSRRYPAVTLDEVEALRPEVVLLPDEPYPFSAEDLPEFYALDIPAARS
                     DDIYLVDGKLLTWYGPRMAGSLMQLSALLRAGQPALPESQE"
     gene            complement(7579..7995)
                     /locus_tag="VFJ72_02865"
     CDS             complement(7579..7995)
                     /locus_tag="VFJ72_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HET7478440.1"
                     /translation="MRTLSAMFCGLVFGLGLAISGMMNPAKVIGFLDVAGDWDPTLAF
                     VMGGALLVAVPAFRFIPKRPRPVLEDDFSLPTKKTLDAPLLGGSALFGIGWGLVGFCP
                     GPAVAALGTGLPVVLAFVAAMLSGMALYSWLSGKAG"
     gene            complement(8010..8477)
                     /locus_tag="VFJ72_02870"
     CDS             complement(8010..8477)
                     /locus_tag="VFJ72_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HET7478441.1"
                     /translation="MAGHPVRHGRVTSGGDMEDFTPISGFVGGLLIGLAVTLMLLLNG
                     RIAGISGIVGGLLTLRGRDIHWRAAFVAGLVLGALAYIATAGETGSVRVLASLPAVLV
                     GGLLVGFGTRLGSGCTSGHGLCGMARFSKRSIAATATFFGVAMITVYLTHHAL"
     gene            complement(8549..9043)
                     /locus_tag="VFJ72_02875"
     CDS             complement(8549..9043)
                     /locus_tag="VFJ72_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HET7478442.1"
                     /translation="MRARPATPEDAETIAYIYNQGIEERIATFETRPRSAEEIRAWFG
                     GPHPTVVVEEDGRVVAFASTSSYRPRECYKGVAEFSVYVARDARGHGAGRLAMQALIS
                     AAERTGLWKLASRVFIENTASRRLLVSLGFREVGVYESHARLDGVWRDIVIVERLLIS
                     AQDP"
     gene            complement(9081..9431)
                     /locus_tag="VFJ72_02880"
     CDS             complement(9081..9431)
                     /locus_tag="VFJ72_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7478443.1"
                     /translation="MPDLHALAVEDFCYLTTTGRATGRPHMIEIWFALEDSTLYMLSG
                     GRDRSDWVKNLRENPQVTVRIADETFEGRARVVEDSEEDELARRLLVEKYERRPGSLD
                     NWRRTALPVAVDLG"
     gene            complement(9432..>11060)
                     /locus_tag="VFJ72_02885"
     CDS             complement(9432..>11060)
                     /locus_tag="VFJ72_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7478444.1"
                     /translation="LWGKVRIVANIGDYEKTYAGFDWETPERFNFGRDVVDRWAAQDR
                     PAMIWLGVDGEERRLDFAQFSVLSNRFANAARELGVQRGDRVMVLLGKVPEWHAILTG
                     LLKLGAIAIPCAPQLRAGDLKFRAEHSGSAAMISGPEGIEAVEKMRSDVPGLKHFVSV
                     GGDHDGWESFDKLMDGAPDEFTAEDTAADEGAFILYTSGTTKHPKGVLHTHGYTHAKW
                     MQAKYWLDLQDGDRLWCTSGTGWAKSIWNVFLGPWSQGTEIFFHEGGFDPAERLDLIE
                     RYNITVLCQAPTEYRLLAKTQELQEANLSSIRHAVSAGEPLNPPVIERWKELHGITIY
                     DGYGQTENTLLVGNYPGVEVRPGSMGKPSPGCDVKVIDMDGNECPPDEPGDIALAGRI
                     PCLFKEYWEQPDATEATFRGGYYLTGDRAYRDADGYMWFVGRSDDVILSAGYRIGPFE
                     VESVLIEHPAVVESAVVPAPDEDRGSVVKAYIVVGKDYEPSDELAKEIQDYCKANTAP
                     YKYPRRIEFISELPKTTSGKIRRVELRQRETADA"
BASE COUNT         2002 a         3477 c         3892 g         1689 t
ORIGIN      
        1 gccagggtgt agacgtcccc gaggctcggc gccttgacgc acagctccag ggagttgagg
       61 aatcggaagg cggcttcacg actctccgct gccagctcga aggagaccag gcctccggta
      121 tcggcgagca cacgtcgggc gacttcggta tccgggtggt ctttgaggga ggggtgattg
      181 accttcgtga tcttcggatg agaggcgagg cgggaggcta tctcgcgcgc gttctcgcac
      241 tggcgggaca tgcggagcgg gagggtcttc aggccccggt gagcgagcca ggactcgaac
      301 ggcccgatca cgccgccgac cagcttccgt agttgctcta ccgctccgtc gtacggagcg
      361 cccgtgacga cgaggccggc ggtgatgtcg ccgtggccgg agaggtactt ggtcgcgctg
      421 tggaccacga tgtccgcccc gaggtccagg gaccgctgca ggtacggcgt gccgaaggtg
      481 ttgtccacta tcagggccga gcccgcagcc cgcgttacct ccgcgatggc cggcacgtcc
      541 gcgacgcgca ggagcgggtt ggagatagac tcgatgacca cggcgcgggg cctcagttcc
      601 tcgacctttt tctcgacggc ctccaggtcg tagcaattca cgaaccggac ctccacgccc
      661 gccgcgccga agacctgcac caggagcgtc gccgtggagc cgtagagttg ctcggcggcc
      721 agcaccgtgg cgccgcccgt cagctccgcc gccgcgagcg ccgcgtgcat cgccgccatc
      781 ccggagccga aggcgaacgc gcgcgcctcc ggcgaacctt ccagcgacgc tatcgcccgc
      841 tcgaaggcgg cgacggtcgg gttgtcgtag cgcgcgtagc tgtagcccgg ctcctcgccg
      901 ccgagcaccc ggtccgtcgt ctctatatta gggtgggaga aggtcgtcga ggcgtagatc
      961 ggcgtcgaga cgggatagaa ggcgctcggc tcgtttgctc ccgcctcctc gtgtttggcg
     1021 cggcgctcgc cggagtggac ggcgcgggtc tcgaaacgtc tctggttatc ctgatccgaa
     1081 ctcatggtgc cctatactaa accaccagcg catacctttt gggaggagca gaggatggaa
     1141 gaccggataa gttatttcga gcgcatgctg gcggacaacc ccaacaaccc cacgggcctg
     1201 atggcgctgg cgaacgagta cggcaaggcg ggccgcgacg aggacgaggc ggcggtcttg
     1261 cagcggtacg tggaggcgca cgaggacgag gggaacgctt acgcccggct cggggaggtg
     1321 ctggcgcggc tcgggcgcga ggacgaggcg cggtcggcct acgagagagg gatcggccag
     1381 tccgagaagc acggccacag cgggatggcc gacgatctgc ggctcgcgtt gctcaggctc
     1441 aacgagtagg cctgcaaagc ccgtgtttag ggtaagtacg gcggatgtga tgtggggtat
     1501 gattagggac attacgatgg ttagttgcct agcgaagagc gcgaagcaag aaggcgtgga
     1561 gatgagcccc gccgcctttc gcgcaccgtt agagattcgc tgcaaaacaa gttaccgcaa
     1621 cgtgagggtg tgatcctgtt ggcaaacctg tccgagagag agttttttgg gccaaacctc
     1681 ggctatgtgt tggagctcta tgaacgctac cgcgaagacc ccggctccgt ggacgagagc
     1741 gcgcgcgagt tcttcgagac ctggagcccg ccgcgtcccg gcgcgggcat agccggcacg
     1801 aacgggcagg tagtccccga ggtggacgtg gagaaggccg tgggggcctc gaagcacgtc
     1861 cggcacataa gggacttcgg gcaccgggcg gctcaactgg acccgctggg gagcgagcct
     1921 cccggagacc cgacgctcaa tcctgaattt tacaggatca gcgacgagga cctggagact
     1981 ctaccggagc tcatcatcgg gggaccgatc gccgagcgca cgtccaacgc ccgggaggct
     2041 gtggaggagc tacgcaagct ctactgcaac accaccggct acgacttcgg gcacgtgcac
     2101 gaccccgacg agaggttctg gctgcgcgag gccgtcgagt ccgggcggtt cgcaaagagg
     2161 atggagggcg aagaggcgaa gcggctcctg cagaggatca cgcgggtaga cgccttcgag
     2221 aagttcctcc acaagacgtt cctcgggcag aagcgcttct ccgtcgaagg caacgacatg
     2281 gtcgtgccga tgctgaaccg cctgatccgg tccgccgccg acgtggggac cccagtggtg
     2341 acgctcggca tggcgcaccg gggtcgcctg aacgtgctgg cgcacgtgat ggacaagtcg
     2401 tactccaaga tcttcggcga gttccagcag ccggacaagg gcgagcaggc gtcggccgcg
     2461 ggcagtcccg gcggggcatg ggtgggggac gtgaagtacc acctcggcat ccggggtttc
     2521 cacctggatg atgcggaagc cgacgaagag gtcctcatca acctcgcgcc caacccgagc
     2581 cacctggagc acgtcaaccc tgtggtcgaa gggatggccc gcgccgcgca ggaggagcgg
     2641 agtgaggcag gaccgccgcg cctggatgtc gaagcggcgc tgccgataat ccttcacggc
     2701 gacgcggcgt tcccggcaga gggtgtctcg gcggagacgc tgaacctcag caggctgccc
     2761 ggctacaaga ccggcgggac catccacatc atcaccaaca accagatcgg gttcaccacc
     2821 gagaggcagg acgcgcgctc cacgacctac gcgagcgacc ttgccaaggg gttcgagatc
     2881 ccggtggtcc acgtcaacgc cgacgacccg gaggcgtgtc tggccgtgac gcggatggcc
     2941 cacgcctacc gcgaggagtt cgacaaggac ttcatgatcg acctggtcgg ctaccgccgc
     3001 ttcgggcaca acgagggcga cgaaccggcc tacacccagc ccctgatgta cgagaccatc
     3061 cgcaaccacc cgacggtccg cgagctgtgg gcgaaggagc tcgaacggcg cggcgtgatc
     3121 gaggagggcg aggccgacgc tatgttcgag gagatcatgg cggacatggc cgaggtacag
     3181 aagaacccgg acgagggcct caccgaggaa gacgtcttta ccgacgagcc gtacactccg
     3241 ctcgcggaga tcccggagac cgccgtcccg gccgagcggc tcgcggagct gaacaaggcg
     3301 cttctggaac gccccgaagg cttcacgccg aactccaagc tggagcgtct gatgcggaag
     3361 agccgtggcg aactcaacgg cagcgatagc tccgctggca tagactgggc gcacgccgag
     3421 acgctggcct tcgcgtcgct gctggaggac ggcaccccga tccgcatcac cggacaggac
     3481 acggagcgcg ggacgttctc ccagcgccac gcggtcctct acgacgcgaa gaccgacgag
     3541 cgttacgtgc cgctccagaa catcccgcag gcgaagtctt ctttctccat ctacaatagc
     3601 ccgctctcgg agatagcggt aataggcttc gagtacggtt actcggtgag ctcggaggac
     3661 acgctggttc tctgggaggc ccagtacggc gacttcgcca acgtcggcca gccgatgatc
     3721 gaccagttca tcgtctccgg gcaggccaag tggggccaga cctcggggct cgtgatgctc
     3781 ttgccgcacg gctacgaggg gcaagggccg gagcactcca gcgcccgcct ggagcgctac
     3841 ctgcaactcg ccgccaacga gaacgtgcgg atagccaacc tcaccacggc ggcccagtac
     3901 ttccacatcc tgcgcgccca ggccgcgctc aagaaccaga agcgtccgct catcatcatg
     3961 gcgccgaaga gcctcttgcg tcatccgatg gccgcctcga agctggagga tttgtccgag
     4021 gactcgttcc atccggtgct cgatgacgcg gaggcgcggg agagggcgga gtcggtcgag
     4081 cggctcatcc tgtgtaccgg taagatcttc acggagctag ccggtagcga gtaccgcgag
     4141 gaggcggtgg ataccgctat cgcccggata gagctactgt acccgttccc ggaggagcgg
     4201 gtgcgcgacc tgctcgacgg ctatccaaac ctgcgggaga tactgtgggt ccaggaggag
     4261 ccgaagaata tgggcgcctg gtcgttcgtg gagccgcggc tgcgcgacat cgccgatgag
     4321 ctgcccatac tgtacgtcgg caagcccgga cggcccagcc cggctcaagg gtcggcgaag
     4381 ttccacaagc aggagcacgc cgagatcatc cgtatggcct tcgagagcgt gaacgaggac
     4441 ctggatgacg agggttcggt ggatgagacc caggtacggg cttcggccaa gagcgagtag
     4501 gactgtgtcg caggtaaata agatcagaga ggaagagtga gccgtggcgg ttgagataca
     4561 cgttccggag cttggggagt cggtcgccga cgcgaccgtc ggcaaatggt tgaagagcga
     4621 gggagacgcc gtgaagtccg gcgacccgct cgtcgagctc gagaccgaca agatcaactt
     4681 cgaggtagaa gccgagcagg acggcgtcct ggagtccatc gccagcaagg aaggcgacac
     4741 cgtcaacgtc ggtgacgtaa tcggcaccat cggcgagggc gaagccgcag gtgacggcca
     4801 gcccgaagat tccggcgagg aagaggaggg ctccgaggag caggcccaga ccgagcagcc
     4861 ggaggccgaa gcggagaccg aagaggacga ggcgccggaa gaggaagccg gcggcgagga
     4921 ggccaacggg caccgggaga tggaggacgg cgctggccgg gcctcgccct cggtcaggaa
     4981 gctggccgag gagtacggcg tccagctcgc cgaggtctcc ggctccggct ccggcgggcg
     5041 cataacccgc gacgacgtgg agcgtttgat ccgggagaag ggccagggcg gcggccaggg
     5101 cggcggcgcc gagaagaccg agcagcccga gcgcgacggc aaggccgaga agccggcccc
     5161 cgcctcccag aacggccggg cggacctcga agagcgggtg cgcatgtcgc ggcgccgtct
     5221 caccatcgcc cagcggctcg tcgagagcca gcagacggcc gccatgctca ccaccttcaa
     5281 cgaggtggac atgagcgccg tcatggaact tcgcaagcgc cgcaaggagt ccttccagga
     5341 gcggaccggc gccaggctcg gcttcatgag cttcttcgcc aaggcggcga taggggcgct
     5401 caaggcgttc ccgaaggtca acgccgagct tcaggacaat gagttggtcc tcaagaagta
     5461 ctacgacctc ggggtggcgg tctctaccga tgaggggctc gtggtgccgg tcttgcgcga
     5521 cgcggaccgc aagagcttcg ccgggataga gcagggtata gccgacctcg caacgaaggc
     5581 gcgcgacggc aagctcgggc tcgaagacct gcggggcggc acgttcacca tcaccaacgg
     5641 cggaatcttc gggtccctgc tctcgacgcc gatcctgacc atgccgcagg tagcgatcct
     5701 ggggatgcac aagatccagg aaaggccgat ggtcgtggac ggcgagatca ccgtccgccc
     5761 gatgatgtac gtggcgctct cctacgacca ccgcgtcata gacggcgcgg aggcggtcgg
     5821 cttcctggtc cgcatcaagg agcttataga agacccggag tctctgctgc tggaaggcta
     5881 gcggatgccc gatggagggc tcgtagaacg gctggtctcc ggagacaagc gggcgctggc
     5941 ccgcgtgatc tcgaagatag agcgcgagga cgaggacgcg ccagggatca tcgcggagat
     6001 ctacccgagg accggcgacg ccgtgacgat cggcttcacg ggaccgccgg gggtcggcaa
     6061 gagcagcatt atcgcgaagc tgatcaagct ctaccgcgac gaggacaaga cggtgggggt
     6121 ggtctcggtc gacccgtcga gcccgttctc gaagggcgcc atcctgggcg accgaatccg
     6181 gctctcggac cacttcctgg accctggggt gtttatccgg tcgatgggga gccgggggca
     6241 cctggggggg ctggcgggcg gctcgcggct cgcggccctg gcgatggagg cctacgggtg
     6301 cgacgtggtg ctctacgaga cggttggggt ggggcagggg gaggtcgagg tggcgtcggc
     6361 ggcggacacg gtcgtgctgg ctttgcagcc gggggcgggg gacgcggtgc aggcgctcaa
     6421 ggctggggtc atggagatag cggacgtgtt ctgcatcaac aaggcggacc acccgcaggc
     6481 gaagaacgcc gcctcggagg tgcggtcgat actggagatc ggccaggagc tcgaccccga
     6541 cccgtggttc ccgccgatag tgatgacgcg cggcgacacc ggcgagggcg ttgaggagct
     6601 gaaggagacg atacagaagc accgctccta cctgaaggag agcgggaagc tggaggagcg
     6661 gcggcgggcg gctctgcggg agttcgtcgt atcgtgggcg acgaaccggc tggagaagga
     6721 gatgcacgag cggctggacc gggaggacac gcaggtcatg gagcaggtct acgaacggag
     6781 tctcgacccg atctccgcct cggagcgcat cttcaaggaa gtctagaacg tggcgccccg
     6841 gcgcatcgtc agcctggtgc cgagcctgac cgaagcgctc ttcgcgttcg gggccggaga
     6901 caggatcgtc ggacgcaccc gctactgcac gcagccgccg cgagcggtcg gcaggatacc
     6961 gaaggtcggc gggacgaaga aggttgacgt agcgcgcgtg ctgcaactgg agccggacct
     7021 cgtcgtcgcc gtccgggaag agaacacgcg gggggacgtg gaggcgctga tgcaggcgga
     7081 cgtcccggtc ttcctcgggt cgccggcgac gatcgcggaa gcggtaaaga tgctgcggga
     7141 gcttgccggg agggtcgagg ctccactggc cggggaggtg ctggagccca tcgagcggac
     7201 gctgggaagg ttgcgggact cggcaaaaga aacgcggcgg gtgttcgtcc cgatctggaa
     7261 gcacccgtac atgagtgtcg gccgagacac ctacgtccac gacgtcctga agacgtgcgg
     7321 cggcgagaac gtgtgcggcg gttcgaggcg ctatccggcg gttacgctgg acgaggtgga
     7381 ggcgctgcgg ccggaggtcg tgctgctccc ggacgagccg tacccgttct ccgcggagga
     7441 cctgccggag ttctatgcgc tcgacatacc cgccgcccgt tcggacgaca tctacctcgt
     7501 ggacggcaag ctgctcactt ggtacgggcc gcggatggcg gggagcctga tgcagctatc
     7561 cgcgttgctg cgggccggtc agccggcctt gccggagagc caggaataga gagccatccc
     7621 agagagcatc gccgccacga aagccagcac gaccgggaga ccggtgccga gggccgctac
     7681 ggccgggccg ggacagaagc ccacgaggcc ccagccgatg ccgaagaggg cggagccgcc
     7741 gagcaggggc gcgtccagag tcttcttcgt tggcagggag aagtcatctt cgaggacggg
     7801 gcgcgggcgc ttcgggataa agcggaaggc cgggacggcg acgaggagcg cgccgcccat
     7861 gacgaaagcc agggtcgggt cccagtctcc ggcgacatcg aggaagccta tgaccttggc
     7921 ggggttcatc atccccgaga tggcgagccc cagcccgaag acgaggccgc agaacattgc
     7981 ggagagtgtc cgcaccgcgc cggaggccat cagagagcgt ggtgggtcag gtacacggtg
     8041 atcatcgcca cgccgaagaa ggtggcggtg gcggcgatgg agcgtttcga gaagcgggcc
     8101 atgccgcaga gcccgtgccc gctggtgcag ccggagccga ggcgggtgcc gaagcctacc
     8161 aggagcccgc cgacgaggac cgccggtaac gaggcgagca ccctcacgga gcccgtctcg
     8221 cccgcggtcg caatgtaggc gagcgcgccg agcacgaggc ccgcgacgaa cgccgcccgc
     8281 cagtgtatgt cccggcctct caacgtcaac aggccgccga cgatgccgct gatgccggcg
     8341 atgcggccgt tcagaaggag catcagcgtg accgccagcc cgatgagtag cccgccaacg
     8401 aaaccgctga tcggggtgaa gtcctccatg tcgcctccgc tcgtcactcg accatgccgg
     8461 acgggatgcc cggccacata aggatatctc ccggaggttc accctgacaa gggaaaatgt
     8521 ttgcgctatg ggcgtccagt ggggaaggtt acgggtcctg cgctgagatc agaagacgct
     8581 ccacgatcac gatgtcccgc cacacgccgt cgagccgggc gtgactctcg tagacgccga
     8641 cctcccggaa gcccagggag acgagcagcc tgcggctcgc ggtgttctcg atgaagacgc
     8701 gcgaggcgag cttccataag ccggttctct cggcggcgga gatcagggcc tgcatagcca
     8761 gccgtcccgc tccgtgacca cgagcgtccc gagccacgta gaccgagaac tcggcgacgc
     8821 ctttgtagca ctcgcgcgga cggtagcttg aagtgctggc gaacgcgacg acccggccat
     8881 cctcctccac gaccacggtc ggatgcgggc cgccgaacca ggcccgtatc tcctccgccg
     8941 aacgcggccg ggtctcgaag gtggcgatgc gctcctcgat gccctggttg tagatgtagg
     9001 ctatagtttc ggcgtcttcg ggggtcgcgg gacgggctcg catggcggca tcctagcagt
     9061 cgaatccccc gtctttctcg ctacccgagg tccacggcga ccggcagcgc cgtgcgtcgc
     9121 cagttgtcca ggctgccggg cctcctctcg tacttctcga ccaggagccg gcgcgccagc
     9181 tcgtcctcct ccgaatcctc gaccacgcgg gcgcggccct cgaaagtctc gtccgcgata
     9241 cggaccgtca cctgcgggtt ctcgcgcagg ttcttcaccc agtccgagcg gtctcgtccg
     9301 ccggagagca tatagagggt gctgtcttcg agtgcgaacc agatctcgat catgtgcggc
     9361 cgtcccgtcg cgcggcccgt ggtcgtcagg tagcagaagt cctcgacggc gagggcgtgg
     9421 aggtccggca tctatgcgtc ggcggtctct cgctggcgca actcgacccg acggatcttg
     9481 ccgctcgtcg tcttcggcaa ctctgagatg aactcgatcc tgcgcgggta cttgtacggc
     9541 gcggtgtttg ccttgcagta gtcctgtatc tccttagcca gctcgtccga cggctcgtag
     9601 tccttcccaa ccactatgta cgccttgacc acgcttcccc ggtcctcgtc cggcgccggg
     9661 accaccgcgc tctccacgac ggccgggtgc tctatgagga cgctctcgac ctcgaacggc
     9721 ccgatcctgt acccggcgga gagtatgacg tcgtccgaac gccccacgaa ccacatgtag
     9781 ccgtccgcgt cccggtaggc ccgatcgccc gtgaggtagt agccgccccg gaaggtcgcc
     9841 tccgtcgcgt cgggctgctc ccagtactcc ttgaagaggc acgggatacg cccggcgaga
     9901 gctatgtcgc cgggctcgtc cggcgggcac tcgttgccgt ccatgtctat gacctttacg
     9961 tcacagccgg gcgagggctt gcccatcgag ccgggccgca cctccacacc ggggtagttg
    10021 ccgacgagca gggtgttctc cgtctggccg tagccgtcgt agatggtgat accgtgcagc
    10081 tccttccatc gctcgatgac gggcgggttg agcggctctc cggcggagac ggcgtggcgg
    10141 atggaggaga ggtttgcctc ctgaagctcc tgcgtcttgg cgagcaggcg atactcggtc
    10201 ggggcctggc agaggacggt tatgttgtag cgttcgatga ggtccaggcg ttctgcgggg
    10261 tcgaagccgc cctcgtggaa gaagatctcc gtcccctggc tccacggccc cagaaagacg
    10321 ttccagatgc tcttggccca cccggtcccg gaggtgcacc agagccggtc gccatcctgc
    10381 aggtccagcc agtacttcgc ctgcatccac ttcgcgtgcg tatacccgtg cgtgtgcagc
    10441 acgcccttcg ggtgcttcgt cgtcccggag gtgtagagga tgaacgcgcc ctcgtccgcc
    10501 gccgtatcct cggcggtgaa ctcatccggg gctccgtcca tcagcttatc gaacgactcc
    10561 cagccgtcgt gatctccgcc cacggatacg aagtgcttga ggccggggac atcggagcgc
    10621 atcttctcga cggcctcgat gccttcgggg ccggagatca tggctgcgga gccggagtgc
    10681 tcggcccgga acttgaggtc gcccgcccgg agctgcgggg cgcaggggat ggcgatggcg
    10741 cccagcttta gtaggccggt aaggatggcg tgccactccg ggaccttgcc gagcaggacc
    10801 atcacccggt cgccgcgctg tacgcccaac tcgcgggcgg cgttcgcgaa gcggttggag
    10861 aggacggaga actgggcgaa gtccaggcgg cgctcttcgc cgtctacacc cagccagatc
    10921 atggccggcc ggtcctgcgc ggcccagcgg tccaccacgt cgcgcccgaa gttgaacctc
    10981 tccggcgtct cccagtcgaa gccggcgtag gtcttctcgt agtctccgat gttcgccacg
    11041 atcctcacct ttccccaaag
//
LOCUS       DASSMB010000040         7300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_9335, whole genome shotgun sequence.
ACCESSION   DASSMB010000040 DASSMB010000000
VERSION     DASSMB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9335"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VFJ72_02890"
     CDS             <1..259
                     /locus_tag="VFJ72_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478445.1"
                     /translation="LAMLRTGMPGRWAGWLALAVALFHVLAALGLAQSGTLAMDGLLA
                     NLAPLVFLVWVVATSVPLLRRAGARSGSAGRAPDDAVHPAT"
     gene            complement(299..1348)
                     /locus_tag="VFJ72_02895"
     CDS             complement(299..1348)
                     /locus_tag="VFJ72_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HET7478446.1"
                     /translation="MTASQAPTRRYAILAEGRFLDANAKTAHGLLRYGKDEVVAVMDS
                     SLAGRSVLEVMPSLRREAPIVGTMEEALAYSPTSILVGLAPPGGRLPGAWIETLRMAA
                     EAGVEIVSGLHQRLAPLFPGKNVWDVREPPEEIPLFSGEGLDVTPKVALTVGTSSAIG
                     KMTATLEIERAAMEAGVTTEFVATGQTGIIIAGWGISVDAVVSDFIAGAAEQLVLRAA
                     KSSPGLILVEGQGSLGHPAYSGVTLGLLHGSCPDCLILCCDASEERIMGLVPRPTPAR
                     AARLYEEVAALVKPAPVVAVSVNTKGLDDAEAEEFVARVADETGLPAADPLRGSATPI
                     LRAVLEAPKTRGGLS"
     gene            complement(1345..2340)
                     /locus_tag="VFJ72_02900"
     CDS             complement(1345..2340)
                     /locus_tag="VFJ72_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HET7478447.1"
                     /translation="MRITVEKLTVHTRRTFAIARGAHDAFERVILTLEEDGATGRGEA
                     APTAYYEQDAAGVEAALRRLSIPDPWDIEGALRGNAHLPPAALAALDAALHDLAAKRL
                     GVPVWRLLGLARPAPVSAYTLAIADLDTTVSEARKLSGYPILKMKVGGRGDIETVRAV
                     AAASEAEPWVDANEAFTPDEVSEAVAELREIGAAMIEQPVPASAGPEALRRVTEAASP
                     VPVIADESAITAADVPKLAGSVSGVNVKLAKCGGIRRALEMVHTARAHGMLVMLGCMV
                     ETSLGIAAAAQVSGLFDYLDLDGAMLLADDPYAGLEYKQGHITVPDSPGLGVEPR"
     gene            2509..3234
                     /locus_tag="VFJ72_02905"
     CDS             2509..3234
                     /locus_tag="VFJ72_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7478448.1"
                     /translation="MGSIAEILIVEDDEVIMGTLAYNLSRNGFGVSQATSGAEALRLA
                     RKMRPDLILLDLMLPGESGIQVCERIRERDQGVVIVMITAKGAEEDKVRGFEAGADDY
                     VTKPFGMKELIARINANLKRSDAGHRGKVLEAGDLQLDTKNFTASVNGEPLPLRLKEF
                     ELLAALAGAPGELRSREELAKEVWGHAGVGSSRTIDVHIRRIRAALAEKSAYEFIHTA
                     RGLGYRFDAKPVAKVGVAAPSTE"
     gene            3239..3982
                     /locus_tag="VFJ72_02910"
     CDS             3239..3982
                     /locus_tag="VFJ72_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7478449.1"
                     /translation="MALEPSGESYSPAELRRVNKIRRDFVANVSHELKTPATSLKLLA
                     ESLEECIDEDPVQARFFASQLKSETERLAQLITDLLDLARLESQERTDYPSLVDVRVV
                     LMTVLARMRRVARRKEITLLWKRFGKAAQYTVRGDETQLTSMFTNLVDNAIKYTPPGG
                     RVEVTGGMNDREIVISIRDTGIGIPEGNLSRIFERFYRVDKARSKATGGTGLGLSIVR
                     HVAQNHGGRVTVESEIGEGTTFTVYLPRG"
     gene            4018..4224
                     /locus_tag="VFJ72_02915"
     CDS             4018..4224
                     /locus_tag="VFJ72_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478450.1"
                     /translation="MPHGQHRNETVPVSLRLREVRGNLPDTLHDAVVSDPQAPLFLAG
                     PRILRAPDGRGSFTRERMAARTLS"
     gene            complement(4304..4537)
                     /locus_tag="VFJ72_02920"
     CDS             complement(4304..4537)
                     /locus_tag="VFJ72_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET7478451.1"
                     /translation="MRQFLINERGERTAVVLPIEEYEELLERLEDAEALREADEVLTA
                     IERGEEDPLPLNEAMEEIEAERRRLRESGGAEE"
     gene            4961..>7300
                     /locus_tag="VFJ72_02925"
     CDS             4961..>7300
                     /locus_tag="VFJ72_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7478452.1"
                     /translation="MGHMRAASLGGAGGLLPIAIGFAALLWIVDSLMDALFFDKDGFI
                     NALLSTDLDELWNRIVIVTVLVLFAIVAQRIINRRRETENKLRESEERFRGLSKASFE
                     GIAISEAGKLLEVNDAFAAIFGYERQEMAGMDALEIVAPESREVVRHNIASGYDNSYE
                     AIGLRKDGSRFDIEGHGKTSTYRDHSVRVTAVRDITERKRTEEALKESEERLRLAMEA
                     GMLGTWYLDLRSGEFIASIQAKAMHGLPPENPLDNQGAIEAVHPDDRAEVASKIERTI
                     SERVPYEAEYRIVWPDGGIRWLRARGRVHGLTEDGGGERLIGVVRDITEGKRAEEELR
                     KSEARARAITETAIDAIITMTADGLIQSFNPAAERVFGYTAEEAVGQPLRMLMPERFR
                     GSHERGFREYLGGREAHVMGKGAVELAGLRKDGIEFPLELSLGEMREGGDALFTGIIR
                     DITGRKRSGRRLEAQYAATRVLGESATLEEASLKILRIVCENLDWEVGEIWEIDQEAD
                     VLRCARHWCVPDVDVSALADDLRCRSFARGDGLPGRVWAAGDPVWIPDVSKEASFVRA
                     DIAAGAGLHAAFASPILLRGEVLGVMCFLGRAVREPDEAVLDMMDTIGTQIGQFIERR
                     RAEKEVREAEERFRSAFDDAAIGMGLNSPDGRFLRVNRALCEMLGRPEEELLSLTFAD
                     ITHPDDVDTSREQVRRLLDGETDGYRMEKRYLHADGSVVWVALSVSGIRDAEGRARYL
                     AAQMQDITERKAAEERLAASEAELRALFAAMTDVILVLDN"
BASE COUNT         1228 a         2361 c         2513 g         1198 t
ORIGIN      
        1 gctcgctatg ctcaggacgg gcatgcccgg caggtgggcc ggctggctgg cgctcgcggt
       61 cgccctcttc cacgtgctgg cggcgctggg cctggcgcag agcgggacgc tcgccatgga
      121 cggtcttttg gcgaacctgg ccccgctggt gttcctggtg tgggtcgtcg ccacgagcgt
      181 gccgctgttg agaagggcgg gagcgcgctc gggctccgcg ggccgcgcgc cggacgacgc
      241 cgttcacccg gccacgtagg ataccggatg ggcgggccga gtaccgttcg tcacttcgtt
      301 aagatagccc gccgcgggtc ttcggggctt caagcaccgc ccgtagtatc ggggtggcgg
      361 agccccgcag cgggtcggcg gcgggcagcc cggtctcatc tgctacccgc gcgacgaact
      421 cttcggcctc cgcgtcgtcc agacccttcg tgttgacgct gacggcgact acgggcgcgg
      481 gcttgacgag ggcggcgact tcttcgtaga ggcgggcggc gcgggcgggg gttgggcgtg
      541 ggacgaggcc cattatcctc tcttccgagg cgtcgcagca gaggatgagg cagtccgggc
      601 aggagccgtg cagcaggccg agcgtaacgc cggagtaggc cgggtgtccg agggagccct
      661 ggccttccac caggatcagg cccggcgaac tcttcgccgc ccgcaggacg agctgctccg
      721 ccgcccccgc aatgaagtcc gagacgacgg cgtccacgga gatcccccac ccggctatta
      781 tgatgccggt ctggcccgtc gccacgaact cggtcgttac gcccgcctcc atcgcggccc
      841 gctctatctc cagggtcgcc gtcatcttgc cgatggcgct gctggtcccg acggtgagcg
      901 cgaccttcgg cgtgacatcc aggccctcgc cggagaagag cggtatctcc tccggcggct
      961 cgcgcacgtc ccagacgttc ttgccgggga agagcggcgc gaggcgctgg tgcaggccgc
     1021 tgacgatctc cactcccgcc tccgccgcca tccgcagggt ctcaatccac gcccccggca
     1081 gccgcccgcc cggcggggcg agcccgacca ggatggaggt cggcgagtaa gctagcgcct
     1141 cctccatcgt cccgactatc ggggcttcgc ggcgcaggct cggcatgact tcgaggacgc
     1201 tcctcccggc gagcgaggag tccatgacgg cgactacctc gtccttgccg tagcgcagga
     1261 ggccgtgggc ggtcttggcg ttggcgtcga ggaagcgccc ttcggcgagg atggcgtatc
     1321 ggcgggtggg cgcctgggat gcagtcatcg cggctccacc ccgaggccgg gactgtcggg
     1381 cacggttatg tgtccttgct tgtattcaag acccgcgtag gggtcgtcgg cgaggagcat
     1441 ggcgccgtcg aggtccaggt agtcgaagag gccggagacc tgggccgccg ccgctatccc
     1501 gagcgaggtc tcgaccatgc agccgagcat gacgagcatc ccgtgggcgc gggcggtgtg
     1561 gaccatctcc agcgcccgcc ggatgccgcc gcacttcgcc agcttgacgt tgacgccgga
     1621 gacagagcca gccagcttcg gcacgtcggc ggcggtgatg gcgctctcgt ccgcgatcac
     1681 gggcacgggg ctcgcggcct ccgtgacgcg ccgcagcgcc tccgggccgg cggaggcggg
     1741 gacgggctgc tcgatcatgg ccgccccgat ctctctcaat tccgccacgg cttccgaaac
     1801 ctcgtccggc gtaaacgcct cgttggcgtc cacccatggt tccgcctccg aagcggcggc
     1861 gaccgcccgc acggtctcta tgtctcccct gccccccacc ttcatcttca ggatgggata
     1921 gccggagagc ttcctcgcct ccgagacggt cgtgtcgagg tcggctatgg cgagggtgta
     1981 ggcgctcacg ggcgcggggc gggcgaggcc gaggagccgc cagaccggca cgccgagcct
     2041 cttcgcggcg aggtcgtgta gggcggcgtc cagggcggcg agcgcggccg gcgggaggtg
     2101 ggcgttcccg cgcagggcgc cctcgatgtc ccaggggtcg gggatgctga ggcggcgcag
     2161 cgcagcctcc acgcccgcgg cgtcctgctc gtagtaggcg gtgggtgctg cctcgccccg
     2221 gccggtcgcg ccgtcttctt cgagggtgag gatcacgcgc tcgaaggcgt cgtgggcgcc
     2281 gcgggcgatg gcgaaggtcc gtctagtgtg gaccgtgagc ttctctaccg tgatccgcat
     2341 gcgcctcccg gccgttctgg tcccgtctct gccagccgca ttctagcggg atagaagcat
     2401 tccttcccgt ccgggaaccg cgcatgttaa aggcttgtta aaagagtgaa gcatggcaat
     2461 ccggcgttta tactccgggt gtagccgaac ttttggaggt agcgtctggt ggggagcatc
     2521 gcggagatcc tgatagtcga ggacgacgag gtgatcatgg gcaccctcgc ctacaacctc
     2581 tcgcgcaacg gttttggtgt gagccaggcg acctcgggcg cggaggcttt gcggttggcc
     2641 cgcaagatgc gcccggacct tatcctgctc gacctgatgc tccccggcga gtcggggata
     2701 caggtgtgcg agcgcatccg ggagcgggac cagggcgttg tgatcgtcat gatcacggcg
     2761 aagggcgccg aggaggacaa ggtgcgcggc ttcgaggccg gcgcggacga ctacgtcacc
     2821 aaacccttcg ggatgaagga gcttatcgcg cgcatcaacg ccaacctcaa gcgctccgac
     2881 gcgggccatc gcggcaaggt cctggaggcc ggggaccttc agttggacac gaagaacttc
     2941 accgcgagcg tgaacggcga gccgctgcct ctgcgcctga aggagttcga gcttctggcc
     3001 gcgcttgcgg gcgcgccggg ggagctcagg agccgggagg agctggcgaa agaggtctgg
     3061 gggcacgcgg gggtcggttc gtcgcgcacg atagacgtgc acatccggcg catccgggcg
     3121 gcgctggcgg agaagtccgc ctacgagttc atccacaccg cccgcggcct cggctaccgc
     3181 ttcgacgcga agccggtagc gaaggtcggc gtggccgcgc cctccacgga gtagaagagt
     3241 ggccctggag cccagcggcg agtcgtactc ccccgcagag ctccggcggg tcaacaagat
     3301 ccggcgcgac ttcgtcgcga atgtctccca cgagctgaag acccccgcca ccagcctgaa
     3361 gctgctcgcc gagagcctcg aagagtgcat agacgaggac ccggtccagg cccgcttctt
     3421 cgccagccag ctaaagagcg agaccgagcg cctcgcccag cttataaccg accttctgga
     3481 cctggcccgg ctggagagtc aggagcggac ggattacccg agcctcgtgg acgtgcgggt
     3541 cgtcctgatg acggtgctgg cccggatgcg gcgggtggcg cggcgcaagg agataaccct
     3601 gctctggaag cgcttcggca aggccgccca gtacaccgtc cgcggcgacg agacgcagct
     3661 cacctccatg ttcacgaacc tcgtggacaa cgccataaaa tacacgccgc cgggcgggag
     3721 ggtagaagtt acgggcggga tgaacgaccg cgagatagta ataagcatcc gcgataccgg
     3781 catcggcatc ccggagggga acctctcccg catcttcgag aggttctacc gggtggacaa
     3841 ggcccgctcc aaggccaccg gcggcacggg cctgggcctc tccatagtcc gccacgtcgc
     3901 ccagaaccac ggcgggcgcg tcaccgtcga gagcgagatc ggagagggca ccaccttcac
     3961 cgtctacctg ccccgcggat agcgcgggag ccgctcctat ccgtatatac cctgcctgtg
     4021 ccccacggcc agcaccgtaa cgagaccgtc cctgtctccc tgcggctgcg cgaggtccgg
     4081 gggaatctcc ccgatacgtt acacgacgcg gtagtctccg acccgcaggc gccactcttc
     4141 ctcgccggtc cccgcatctt gcgcgcgccc gacggccggg gatcattcac cagagagcgt
     4201 atggccgcga ggaccctctc gtagtctctc gtagtctctc gccggaatcc ggctcatgcc
     4261 cttgcggacg cgccgcggaa ggacgacctc gtacacggga ctgttactct tcggctccgc
     4321 cagactccct caaccggcgg cgttcggctt cgatctcctc catagcctcg tttaggggga
     4381 gtggatcctc ctccccacgc tctatggcgg tcaggacctc gtccgcctcc cggagagcct
     4441 cggcgtcctc caggcgttcc agcaactcct cgtactcctc gatgggcagc acgacggcgg
     4501 tccgctcccc gcgctcgttg atgagaaatt gccgcatccc tcgtacctcc tcggtcgtcg
     4561 gcgtcccgga ctatctccgg cccgtgagcc ggtcaccggc ccctgctctc ggattataga
     4621 ctcccgcaac accggcatgg acctcccgct ccaaggccac cggcggcacg ggcctgggtc
     4681 tctccatagt ccgccacgtc gcccagagcc ctctctcccg ctctatactc tcgcatgaaa
     4741 cagctgttat cagaacacgt gcgaagatcc gcaaagagat ctgccagcag gagcctgtaa
     4801 ttcaagttcc gggtcaggtc agtcgataac gtataaagta tcgtaaggat ctcctggagt
     4861 ggtggaaacg tattattgaa gagttcaaga acagagggac taatgagcta cgcatccatt
     4921 gccggttgcc ttcgggcggg tctgatcgga gagtcatgag atgggacaca tgagagcggc
     4981 aagtctcggc ggggcagggg gtctgttgcc gatcgcgata ggcttcgcgg cgcttctgtg
     5041 gatagtagat tccttgatgg acgccttgtt tttcgacaag gatgggttta ttaacgctct
     5101 attatccacg gacctggacg agttgtggaa tcgtatcgtg atcgtcacgg tgctcgtact
     5161 cttcgccatc gtcgcccagc gcataatcaa caggcgccgg gagaccgaga acaagttgcg
     5221 tgagtccgag gagcgtttcc gcggcctgtc aaaggcgtcg ttcgagggca ttgccataag
     5281 cgaagccggc aagctgctag aagtcaacga tgccttcgcg gcgatattcg gatacgagag
     5341 gcaggagatg gccggtatgg acgcgctaga gatagttgcg cccgagtcgc gcgaggtggt
     5401 gcggcacaat atagcctccg gttacgacaa ttcctacgaa gcaatagggc tcaggaaaga
     5461 tggttcgcgc ttcgatatag agggtcacgg taagacctcc acgtaccggg atcacagcgt
     5521 ccgcgtaacg gcggtgcgcg atatcacgga gcgcaagcgg acagaggaag ctctaaaaga
     5581 gagcgaggag cggctgcggc tggcgatgga ggccggcatg ctcggtacgt ggtacctcga
     5641 cctgcgaagc ggagagttca tagcctcaat ccaggccaag gccatgcacg gcctcccgcc
     5701 cgagaaccct ctggacaacc agggagcgat agaagccgta catccggatg accgggcgga
     5761 agtagcaagc aagatcgagc ggacaatctc tgagcgagtg ccttacgagg ccgagtaccg
     5821 gatcgtgtgg cccgatggcg ggatccggtg gctccgcgct cgcggcaggg tccacggcct
     5881 taccgaagac ggtggcgggg agcgcctgat cggagtcgtc cgggacatta cggagggcaa
     5941 acgggcggaa gaggagctgc gaaagagcga ggcccgcgcc cgggccatca ccgagacggc
     6001 gatcgacgcc ataatcacca tgaccgccga cggcctgatc cagtccttca accccgccgc
     6061 tgaacgtgtc ttcggctaca cggcggagga agcggtcggc cagcccctgc gcatgctgat
     6121 gcccgagcgg ttccggggat ctcacgagcg aggctttcgc gagtacctcg gcggccggga
     6181 agcgcatgtg atgggcaaag gagcggtgga gcttgcgggg ctcaggaaag atggaatcga
     6241 gtttccgctg gagctgtcgc tgggcgagat gcgggaggga ggtgacgccc tgtttaccgg
     6301 cattatccgc gacatcacgg gacgcaagcg ctccgggcgg cgtctcgaag cccagtacgc
     6361 cgcgacgcgc gtcctcgggg agtccgcgac gctggaggaa gcgtccttaa agatcctgcg
     6421 gatcgtctgc gagaatttgg actgggaggt cggcgagatc tgggagatcg accaggaggc
     6481 cgacgtgttg cgctgcgccc ggcactggtg cgtacccgac gtggacgtct cggcgctcgc
     6541 ggacgacctc cggtgccgca gcttcgcccg cggcgacggg ctccccggcc gcgtctgggc
     6601 cgccggagat ccggtctgga taccagacgt ctccaaggag gccagctttg tgagggcgga
     6661 catcgcagcc ggggccggcc tgcatgccgc tttcgcgtcc ccgatcctgc tgcgcggaga
     6721 agtcctgggc gtgatgtgct tcctcggccg ggcggtccgc gagcccgacg aggcagtgct
     6781 ggacatgatg gataccatcg gcacccagat cggccagttc atagagcgcc ggagggccga
     6841 gaaggaggtg cgcgaggcgg aggagcgctt ccgcagcgcg ttcgacgacg cggccatcgg
     6901 catgggcctc aactccccgg acgggcgctt tctgcgggta aaccgcgcct tgtgcgagat
     6961 gctcgggcgc cccgaggaag agctgctctc cctgaccttc gccgacatta cccatcccga
     7021 cgacgtggat accagccggg agcaggtacg gaggctcctc gacggcgaga cggacggcta
     7081 ccggatggag aagcgctacc tgcacgcaga tggaagcgtg gtctgggtcg ccctgagcgt
     7141 gtcgggcatc cgagacgcgg agggccgcgc gcgctacctc gccgcccaga tgcaggacat
     7201 caccgagcgc aaggcggcgg aggagcggct cgcggcctcc gaagccgaac tgcgagcact
     7261 tttcgccgcc atgaccgacg tcatcctggt cctcgacaat
//
LOCUS       DASSMB010000041         4775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1149261, whole genome shotgun sequence.
ACCESSION   DASSMB010000041 DASSMB010000000
VERSION     DASSMB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149261"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="VFJ72_02930"
     CDS             complement(<1..1140)
                     /locus_tag="VFJ72_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HET7478453.1"
                     /translation="MRTQVGIIGGGPAGTLLSHLLHLQGIESVVLELRGREEVETTVR
                     AGVLEHATAELLKETGVGERMEREGAVHHGIELRFDGRGHRIDFADLTGKSITVYGQQ
                     EVVKDLYAARLNAGGEILFGAAATGIENLDTDEPKVRFRRGARLDLEAGGIRFQEGSE
                     EEELVCDYVAGCDGFFGASRPAIPEAIRREYTRTYPFGWFGILVEGPPSTEELIYALH
                     ERGFALVSTRSPEIQRLYFQCDPNDSMENWPDEKIWAELQARLETEKGDWTLKEGRIF
                     QKNIVQMRSFICEPMRHGRLFLAGDAAHIVPPTGAKGMNLAVADVRVLCRALTEFYES
                     GRTDLLDSYSETCLRRVWKASRFSWWMTSMLHRFHDDDEFRHRLQV"
     gene            complement(1198..2685)
                     /locus_tag="VFJ72_02935"
     CDS             complement(1198..2685)
                     /locus_tag="VFJ72_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478454.1"
                     /translation="MMEASGEVKTAPYPGYRTLWIWLALGWVASGVDRTITGPIMSYI
                     IDSKAPILQGVENPFAVGGLVGSLLFAGYMLMQFPGGYVGDRLGHRTVIVISIIWAGV
                     ATILSGLMTALLGLVAMRVITGLGAGLFYSNDRSVITHQTPFEKRSLGMGFVITGLSI
                     GITLAFLLAAPLIGLGSSVFGAGGAWRMPFIILGIGAIIVGVGMAIFFRGQRGEHEFR
                     PAYPAALRRLGAYAAVFFVAVVGIYFVATLAGLPEWAVALLELVVALLLVGFIFVRLS
                     GEVSPVLYNRDLLLIYIAAIAILWNLWFFGFWSVSIISDAAHSSFLQGALTAAFNAGA
                     GILGFPAGGWLADYAKRKGWGRKRMLVLFTLIQGLLTIGFGFYIMNGGKSLLVLGVLL
                     FTASLFFNALQPMSHALTADLVPSAAYLGAAFGLWNLIGEMGAVLSPAISGTLRDATG
                     NWNGAVMLDGAIILISFVLLLFVREPRREREEPVPAGARLGGEPG"
     gene            complement(2755..4404)
                     /locus_tag="VFJ72_02940"
     CDS             complement(2755..4404)
                     /locus_tag="VFJ72_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7478455.1"
                     /translation="MKVYEAVAETLGRLGVDAAFGLVGSGNFGLIDHMTRQCGVAYHA
                     SRHEAAAVAMADGYARVSGNLGVCTVHQGPGVTNTLTALTEAVKARTPLLLLAGDTAT
                     TALYQNLDVDQDAVVGSVGAGVERIRDADTAIADIARAARRAALERRPIVVSLPIDVQ
                     AQECEAEDPPAFAENLASAPRPSEEAVSEVAGLLDSSSRPVIVAGRGAVLARARPQLE
                     ALGGRIGALFATSMMAKGFFSGNTFDLGVSGGFSSPLAAHLLGEADLVLSFGASLNTW
                     TTNHRRMFSPSSRIVHCDLEASAIGRIQPVTLGVIGDAASTAEALLEELDGRGASYEG
                     FRTGAVAREIEEFRWEDEFEDAGTEDTVDPRSVLMALDAILPEQRTVAVDCGHFAGFA
                     AMHLRVPDAAGFVFAEAFQAVGLGMGAGMGAAVARPDRLTVVVVGDGGLLMSLGELDA
                     AVQLGAPLLVVVMNDAGYGAEVHHFRALGLPTDLALLGERDFAEISAAMGGQGSVVRN
                     VSDLQQVKEWIEAPEGVMVLDCKVNPRVEGEYLKEAFIAEA"
     gene            complement(4401..>4775)
                     /locus_tag="VFJ72_02945"
     CDS             complement(4401..>4775)
                     /locus_tag="VFJ72_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7478456.1"
                     /translation="TRPVEIAGVEIPENARLMLLLASANRDESVFDNPDEFDIRRENA
                     NKHVAFSHGIHFCLGAPLARLEARIAFELLTQRMPDLRLSPDQTFQFDPNMSFRGPKE
                     LWAEWTPTETIRGADGEAESRA"
BASE COUNT          810 a         1662 c         1515 g          788 t
ORIGIN      
        1 gacctgcagg cggtggcgaa attcgtcgtc gtcgtggaag cggtgcagca tggaggtcat
       61 ccaccacgag aaccggctcg ccttccacac ccggcgcagg cacgtctcgg agtacgagtc
      121 gaggaggtcc gtccggcccg attcgtagaa ctctgtcagc gcgcggcaga gcacccggac
      181 gtcggcgacg gcgaggttca tgcccttggc cccggtgggg gggacgatgt gggcagcgtc
      241 gccggcgagg aagagccgcc cgtggcgcat cggctcgcag atgaaggagc gcatctggac
      301 gatgttcttc tggaagatcc tgccttcctt cagagtccag tctcctttct cggtttcgag
      361 ccgcgcctgt agctccgccc agatcttctc gtccggccag ttctccatgg aatcgttagg
      421 atcgcactgg aagtagaggc gctgtatctc cggggagcgg gtgctgacga gggcgaagcc
      481 gcgctcgtga agggcgtaga tcaactcctc tgtcgagggc ggtccctcca ccaggatgcc
      541 gaaccagccg aacgggtagg tgcgggtgta ttccctgcgg atcgcctccg ggatggccgg
      601 ccggctggcg ccgaagaaac cgtcgcagcc ggccacgtag tcgcagacga gctcttcctc
      661 ttctgagccc tcctggaagc ggatgccccc ggcttcgagg tcgagccggg cgcccctgcg
      721 gaagcggacc ttcggttcgt cggtgtcgag gttttcgatg ccggtggcgg cggctccgaa
      781 gaggatctcc cctccagcat tcagccgggc ggcgtagagg tccttgacca cctcctgctg
      841 gccgtagacc gtgatcgact tgccggtcag gtcggcgaag tctatgcggt ggccgcggcc
      901 gtcgaagcgc agctcgatcc cgtggtggac cgctccctcc cgctccatcc tctccccgac
      961 gcccgtctcc ttgagcaact ccgccgtcgc gtgctccagc acaccggccc ggacggtcgt
     1021 ctccacctcc tcacggcctc ggagttcgag cacgaccgac tcgatcccct gcaaatgcag
     1081 caggtgcgag agcagggtcc ccgccggccc gcccccgatt atccctacct gtgtgcgcat
     1141 gggccttgct cctcctctac ttgtagggct ctctaccagc ggctacccga gcacctgtca
     1201 accgggctcc cctcccagac gcgccccggc cggtacaggc tcttcccgct ctctcctcgg
     1261 ctcccggacg aacaggagca agacgaagct gatgaggatg atagctccgt cgagcatcac
     1321 ggcgccgttc cagttgccgg tggcatcccg gagcgtgccg ctgatcgccg gtgagagcac
     1381 ggctcccatc tcgccgatca ggttccacaa cccgaacgcc gcgccgaggt aggcggcgct
     1441 gggtaccagg tccgcggtta gcgcgtgaga catgggttgc agggcgttga agaagaggct
     1501 cgcggtgaac agcaagactc cgagcaccag caaggacttg ccgccgttca tgatgtagaa
     1561 gccgaagcct atggtgagga gaccctggat cagggtgaac aatacgagca tccgcttgcg
     1621 gccccagccc ttgcgcttgg cgtagtccgc cagccagcca ccggccggga agcccaggat
     1681 gccggccccg gcgttgaacg ccgccgtcag ggctccctgc aggaacgacg agtgcgccgc
     1741 gtccgagatg atggagaccg accagaagcc gaagaaccag aggttccaca ggatcgctat
     1801 ggcggcgatg tagatgagca gcaggtcccg gttatacagc accggggaga cctctccgct
     1861 cagcctcacg aagatgaacc ccaccagcag gagggccaca acgagctcga gcaacgccac
     1921 cgcccactcc ggcagtcccg ccaaagtagc gacgaagtag atccccacta cggcgacgaa
     1981 gaaaacggcg gcgtacgcgc ccaacctccg cagcgcggcc gggtaggccg gcctgaactc
     2041 gtgctcacca cgctggcccc tgaagaatat agccatcccg accccgacga ttatcgcgcc
     2101 gatacccagg atgatgaacg gcatccgcca cgcgcctccg gcgccgaaga ccgaggagcc
     2161 caggccgatc agcggcgcgg cgagcaggaa cgccagcgtg atcccgatcg agaggccggt
     2221 tattacgaac cccataccca ggctgcgctt ctcgaagggc gtctggtggg ttatgacgct
     2281 ccggtcgttg gaatagaaaa ggcccgcccc gaggccggtg atcacacgca tcgccaccag
     2341 acctagcagg gccgtcatga gcccgctcag gatggtagcc accccggccc agattatgct
     2401 gattacgatc accgtccggt gcccgagcct gtccccaacg tacccgcccg gaaactgcat
     2461 cagcatgtag cccgcgaaca acaggctccc caccaacccg cccacggcga acgggttctc
     2521 gaccccctgc aagatgggcg ccttgctgtc gatgatatag ctcattatcg gcccggtgat
     2581 ggtgcggtcc acgccgctcg ccacccatcc caacgcgagc catatccaca gcgtccggta
     2641 tcccggatac ggtgccgtct tgacctcgcc gctagcttcc atcatggtct cctttcccca
     2701 tcgatctatg ctcgctccgc tttccccggc ggtgcaagcg ttgttgtgcc agatctacgc
     2761 ctcggcgatg aatgcttcct tcaggtactc tccttccacc cgcgggttga ccttgcagtc
     2821 caggaccatg accccttcgg gagcttcgat ccactctttt acctgttgta ggtcgctcac
     2881 gttgcgtacc acgctaccct gcccgcccat cgcggccgag atctccgcga agtctctctc
     2941 cccgagaagg gccaggtcgg tcggcagacc cagggcgcga aaatggtgca cctcggcgcc
     3001 gtagccggcg tcgttcatga ccacgacgag cagcggcgcc ccgagttgga cggcggcgtc
     3061 gagctcgccg aggctcatca gcagcccgcc gtcgcccacg acgaccacgg tcagccggtc
     3121 gggacgcgcg accgcggcgc ccatcccggc tcccatcccg agcccgacgg cctggaaggc
     3181 ttcggcgaag acgaaacccg ctgcgtccgg gacgcggagg tgcatggccg cgaagcccgc
     3241 gaagtgtccg cagtctacgg cgacggtgcg ctgctccggc aggatcgcat cgagcgccat
     3301 gagcacggag cgcgggtcca ccgtgtcttc cgtgcccgca tcctcgaact cgtcctccca
     3361 gcggaactcc tcgatctccc gcgcgactgc gcccgtcctg aagccctcgt agctagcgcc
     3421 ccgaccgtca agctcttcga gcagcgcctc cgcggtactg gccgcgtcgc cgatcacgcc
     3481 gagggtcacg ggttgtatgc ggcctatcgc cgaagcctcc aggtcgcagt ggactatccg
     3541 ggacgacgga gagaacatcc tgcggtggtt cgtcgtccag gtgttgaggc tggcgccgaa
     3601 ggaaagtacg aggtccgctt cgcccagcag gtgcgcggcg agcggcgagg agaacccgcc
     3661 ggagacgccg agatcgaagg tgtttcccga gaagaagccc ttagccatca tcgaggtggc
     3721 gaagagagcc ccgatccggc ctcccagcgc ctccagttgc ggacgcgccc gcgccagcac
     3781 cgcgccccgt ccggcaacga tcaccggccg gctcgacgaa tccagcagac ccgcgacttc
     3841 cgagaccgcc tcctcggacg ggcggggagc ggaagcgagg ttttctgcga atgccggcgg
     3901 gtcttccgct tcgcactcct gcgcctggac gtctatgggc agcgagacga ctatgggacg
     3961 acgttcgagc gccgcccgtc gcgcggcgcg ggcgatgtcc gcaatcgccg tgtccgcgtc
     4021 gcgtatccgc tcgacaccgg ccccgacgga accgacgacc gcgtcctggt ccacatccag
     4081 gttctgatat agcgccgtgg tcgccgtgtc gcccgccagc aggagcagcg gtgttcgggc
     4141 cttcacggct tccgtaagag cggtgagcgt attcgtgacc cccggcccct ggtgaacggt
     4201 gcagacgccg agattcccgc tcacgcgagc gtaaccgtcc gccatcgcga cggccgccgc
     4261 ttcgtggcgg gaggcatggt acgcgacgcc gcactgcctc gtcatgtggt cgatgaggcc
     4321 gaagttcccg ctcccgacca gcccgaaggc cgcatccacc ccgaggcggc ccagggtctc
     4381 ggcgaccgct tcgtatacct tcatgcccgg ctctccgcct ctccgtccgc gccccgtatt
     4441 gtctctgttg gcgtccattc cgcccacagc tccttcgggc cgcggaatga catgttcggg
     4501 tcgaactgga aggtctgatc cggggagagc cggaggtcgg gcatcctctg cgtcagtagc
     4561 tcgaaggcga tgcgggcttc gagccgcgcc agcggcgcgc cgaggcagaa gtggatgccg
     4621 tgggagaagg ctacgtgctt gttcgcgttc tcgcgccgga tgtcgaactc gtccgggtta
     4681 tcgaagacgc tctcgtcgcg gttggcggag gcgaggagca gcatgagccg ggcgttttcg
     4741 ggtatttcga caccggcgat ctccacgggg cgcgt
//
LOCUS       DASSMB010000042         4952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_516004, whole genome shotgun sequence.
ACCESSION   DASSMB010000042 DASSMB010000000
VERSION     DASSMB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516004"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VFJ72_02950"
     CDS             <1..870
                     /locus_tag="VFJ72_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7478457.1"
                     /translation="GSPANIAVNVAKLGGASAVVSKTGIGAFGQFIKSELQRYGVSID
                     YLIMDHRNRTTVAFVSSTSGKPDFEIARSGDYLLRPEEISEESVERAKVIHASTFALS
                     REPCRSAVRKVFQLAHEQEKIVSLDPNYSARIWPHHKEARTVLKEMYRYVSITKPSLD
                     DAGRIFSREYEPEQYIEMFHEMGPTTVILTMGKEGTLISEDGHLVGHVPARPIEVEDA
                     TGAGDAFWAGFLTALIRGESPMRCAFFAREVVELKLIRTGPLPLDIDYDELFSRMEEA
                     HTEYEKQVAQAEQ"
     gene            909..3437
                     /locus_tag="VFJ72_02955"
     CDS             909..3437
                     /locus_tag="VFJ72_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7478458.1"
                     /translation="MHVAFINPQGNFDPNDSYWTAHPDFGGQLVYVKELALAMGNLGH
                     KADIVTRRIKDPDWPEFAADTDSYPDAPNVRILRIPCGPDTFLPKEELWPYLGTEFVP
                     RLLDLYEREGSLPDAVTSHYGDGGLCAALIEERTGVPFSFTAHSLGAQKMDKMGIPRR
                     DIGQVDARYHFSKRIIAERLGMNRSSVNITSTSQERFNQYTHNAYRGAVDPTDDARFA
                     AVPPGVALHIFDKNVRYEEEDHVKEHVRASLARDLRADRRGLPCVVASSRLDPKKNHL
                     ALVEAFAGSPTLRASANLVILTGNLENPLEDYRDARDEERAVLDSLMEVIDRARLRGE
                     VSMFAIQGQRALAAAYRHLSGQRSVFALTAQYEPFGLAPLEAMAAGLPAVVTKYGGPS
                     ESMREGDEEYGILVDPTNPEEVGAGLYTLTGNPDRWRRYAEAGYGRVLARYTWERTAE
                     GYLAAISGGGRDAETGREEAATTLHAGDEPGAVSANGSRPTRLPIPLYFTDGASENEP
                     SEDGVSLEALDELYYRLDVLAVGETVVDFISHEMAHSLRTAGQFSRYLGGQPANVALY
                     VAKLGGRSAILSKIGTDYFGEYVEDQLQHHGVNTEALRRTDEAATTNAFVTRTVSVPD
                     FQINRGADALLELRDVADELVERARIVHTSAFALSRDPQRLAVRRAMRLGHRLGKIVT
                     LDPNYDPRVWPDRVEAWEVLAEVLPYVTIVKPSLEDARRLFDYNMDEETLEETALHEL
                     HDLGAKIVILTRSGGMVTVSDGGQVERVGPLPKVQIENVTGARDAFWSALLVAHLDGK
                     GWSEAVCFAHEVASMKLRVEGHVERMIDRGEIYGRLESPAGQRA"
     gene            3443..4522
                     /locus_tag="VFJ72_02960"
     CDS             3443..4522
                     /locus_tag="VFJ72_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478459.1"
                     /translation="MSERKITELPDLIEGLSSEDIILADRIFHVSATTGKLDPPESMR
                     AWIEKSFGSVDRVTAQRIVKVTNRVTLEGTLFNELRASRPLDTAVSVDLDREIAATEG
                     DPFCHPEDGTPADVFGRVKGEHATTASNIAKYDGFHGVVVFDDHNPLHLTPEKVAGYV
                     SVGLEWCRKALEADPEARYPFLMWNCLWRAGGSIIHGHAQVTTTRGMHYPRVERLRRA
                     AMSYRAEHGTDYFTDLHRVHDSLGLGIPLEGVRAFASLSPIKEKEMVVIGGEPGDEAL
                     SRAVGALLEGYVHDLGVRAFNVAFYMPPLGPTQEDWSAFPTVVYIVDRGDPANRTSDM
                     GAMELYAASVVASDPFQVAEVLRHG"
     gene            4709..>4952
                     /locus_tag="VFJ72_02965"
     CDS             4709..>4952
                     /locus_tag="VFJ72_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7478460.1"
                     /translation="MKKDPQNLDTTNKRPYRMGARAEAAEETRRRILNSAIELYTGRY
                     YDQVSLEDVAGRAGVTVQTVLRRFGSKEGLIDAATEV"
BASE COUNT          944 a         1722 c         1527 g          759 t
ORIGIN      
        1 ggctctccgg ccaacatcgc ggtcaacgtc gccaagctcg gcggcgcctc ggcagtggtc
       61 tcaaagaccg gcatcggcgc gttcgggcag ttcataaaga gcgagctcca gcgctacggc
      121 gtctccatag actacctgat catggaccac cgcaaccgca ctaccgtcgc cttcgtctcc
      181 tcgaccagcg gcaagccgga cttcgagata gcccgcagcg gcgactacct gctgcgtccg
      241 gaggagatct ccgaggagtc cgtcgagagg gcgaaggtga tccacgcctc gaccttcgcc
      301 ctctcgcgcg agccctgccg ctccgccgtc aggaaggtct tccaactggc gcacgagcag
      361 gagaagatcg tctccctcga ccccaactac agcgcccgca tctggccgca ccacaaggag
      421 gcgcgaaccg tcctcaagga gatgtaccgg tacgtcagca taaccaagcc gtcgctcgac
      481 gacgccgggc gcatcttctc ccgcgagtac gaaccggaac agtacattga gatgttccac
      541 gagatgggtc cgacgaccgt catccttacg atgggaaagg aggggaccct gatctccgag
      601 gacggccacc tggtcggtca tgtccccgcc cgccccatcg aagtcgagga cgcaaccggc
      661 gccggcgacg ccttctgggc cgggttcctc accgccctga tccggggcga atcccccatg
      721 cgctgcgcct tcttcgcccg agaggtcgta gagctgaaac tcatacgcac gggaccgctc
      781 ccgctggaca tagactacga tgagcttttc tcccgcatgg aagaggcgca caccgagtac
      841 gagaagcaag tagcacaagc cgaacagtaa cggcagaccc cgcatcccaa gaaggggagg
      901 acaacccgat gcacgtcgcg ttcataaacc cgcagggcaa cttcgacccg aacgactcct
      961 actggaccgc ccacccggac ttcggcggcc aactcgtcta cgtgaaggag ctggccctcg
     1021 ctatgggcaa cctgggccac aaagcggaca tcgttacccg ccgcatcaaa gaccccgact
     1081 ggcccgagtt cgccgcggac accgactcct acccggacgc cccaaacgtc cgcatcctcc
     1141 gcatcccctg cggccccgat acgtttctcc cgaaagagga gctgtggccc tacctcggca
     1201 cggagttcgt tccccggctc ctggatctct acgagcgcga aggctcgctc cccgacgcgg
     1261 taacttctca ctacggcgac ggcggactgt gcgcggctct gatcgaagaa cggacaggtg
     1321 tccctttcag cttcacggcc cattctttag gagcgcagaa gatggacaag atgggcatac
     1381 ctcgccggga catcggtcag gtggatgctc gctaccactt ctcgaagcgc ataattgccg
     1441 agaggttggg gatgaaccgc tccagcgtaa acatcaccag cacctcgcag gagcgcttca
     1501 accagtacac ccacaacgcc taccggggcg cagtggaccc gacggacgac gcccgcttcg
     1561 cagcagtccc gcctggtgtc gctctgcaca tcttcgacaa gaacgtccgc tacgaggaag
     1621 aagaccatgt aaaggagcac gtccgggcgt cgctggcgcg cgaccttcgc gcagaccggc
     1681 gaggtcttcc gtgtgtcgtc gcctccagcc gcctcgatcc gaagaagaac catctggcgc
     1741 tcgtcgaggc gttcgctgga agcccgacgc tcagagcgtc ggctaacctc gtgatcctga
     1801 ccggcaacct ggagaacccg ctcgaagact accgtgacgc ccgcgacgag gaacgcgccg
     1861 tcctcgactc gctgatggaa gtaatagacc gggcgcgcct gcgcggcgag gtctcgatgt
     1921 tcgcgatcca gggccagcgc gcgctcgccg cagcctaccg gcatctatcc ggacagcgat
     1981 ccgtcttcgc tctgaccgcg cagtacgagc cgttcgggct cgcgccgctg gaagcgatgg
     2041 cggccgggtt gccggcggtc gtcaccaagt acggcggacc gagcgagtcc atgcgcgagg
     2101 gcgacgagga gtacggcatc ctggtagacc cgaccaaccc ggaggaggtc ggagcgggcc
     2161 tgtacacgct cacgggcaac cccgatcgct ggcggcgcta cgccgaggcg ggctacgggc
     2221 gggtcctcgc ccgctacacc tgggaacgga ccgccgaggg ctacctcgcg gccatctccg
     2281 gcgggggccg cgacgccgag accggccgcg aggaggcggc gacgaccctg cacgcgggcg
     2341 acgagccggg cgcggtttcg gcaaatggtt cgagacctac gcggttaccg atccctctct
     2401 acttcaccga tggcgcttcg gagaacgagc cctcggagga tggcgtctcg ctggaggctc
     2461 tggacgagct gtattatcgg ttggacgttc tggcggtcgg ggagacggtg gtggacttca
     2521 tctcgcacga gatggcgcac tcgctcagga cggcgggaca gttctcgcgg tatctgggcg
     2581 gtcagccggc gaacgtggcg ctgtatgtgg cgaagctcgg cggtcgttcg gcgatactct
     2641 ccaagatcgg gaccgactac ttcggcgagt acgtggaaga ccagctccag caccacggcg
     2701 tcaacaccga ggcgctgcgg cgcacggatg aggcggcgac gaccaacgcc ttcgtgaccc
     2761 gcaccgtcag cgtgccggac ttccagatca atcgcggcgc cgacgccctg ctggagctgc
     2821 gcgatgtcgc ggacgaactg gtggagcgcg cccgcatcgt ccacacctcg gccttcgcgc
     2881 tctcccgcga cccgcagcgt ctcgccgtcc gccgggcgat gcgcctcgga caccggctcg
     2941 ggaagatcgt caccctcgac ccaaactacg acccccgcgt ctggccggac agggtcgagg
     3001 cgtgggaggt cctcgccgaa gtcctcccgt acgtcaccat agtcaagcct tcgctcgaag
     3061 acgcccgccg cctcttcgac tacaacatgg acgaggagac cctggaagag acggctctac
     3121 acgagctaca cgacctcggc gcgaaaatcg tcatcctcac ccgctccggc ggcatggtga
     3181 ccgtctccga cggcgggcag gtagagcgcg tgggaccgct cccgaaggtc cagatagaga
     3241 acgtaaccgg cgcccgcgac gccttctggt cggcgctgct cgtggcgcac ctggacggca
     3301 agggctggtc cgaggccgtc tgcttcgccc acgaggtcgc gagcatgaag ctccgggtcg
     3361 agggccacgt cgagcgcatg atcgaccgcg gtgagatata cggccgcctc gaatctcccg
     3421 ccggccagag agcgtaaccg ccgtgtccga acgcaagata accgaactgc cggacctcat
     3481 cgaagggctc tcgtctgagg acatcatcct cgcggatcgc atcttccacg tctccgcgac
     3541 gaccgggaag ctcgacccgc cggagtcgat gcgcgcctgg atcgagaagt ccttcggctc
     3601 cgtggaccgg gttacggccc agcgcatagt caaagtgacg aaccgcgtca ccctcgaagg
     3661 aaccctcttc aacgagcttc gcgcctcccg gcccctggac accgccgtga gcgtggacct
     3721 cgaccgcgag atagccgcaa ccgagggcga cccgttctgc cacccggaag acggcactcc
     3781 ggcggacgta tttggccgcg tcaaaggcga gcacgccacg accgcctcca acatcgccaa
     3841 gtacgacggc ttccacggcg tcgtggtctt cgacgaccac aacccgctcc acctcacccc
     3901 cgagaaggtc gccggttacg tctccgtcgg cctcgaatgg tgccgcaagg ccctcgaagc
     3961 cgacccagag gcccgctacc cgttcctgat gtggaactgc ctgtggcggg ccgggggctc
     4021 cataatccac ggccacgcgc aggtgaccac cacccgcggg atgcactacc cgagggtcga
     4081 gcgcctccgc cgcgccgcga tgagctaccg cgcggagcac ggcacggact acttcaccga
     4141 cctgcaccgc gtccacgact ccctgggact cggtataccg ctcgaaggcg tgcgggcgtt
     4201 cgccagcctc tcacccataa aggagaagga gatggtcgtg atcggcggcg agcccggaga
     4261 cgaggccctc agccgcgcgg tcggcgccct gctggagggc tacgtccacg acctcggcgt
     4321 ccgcgccttc aacgtcgcct tctacatgcc cccgctcggt cccacgcaag aggactggtc
     4381 cgctttcccg accgtcgtct acatagtgga ccggggagac cccgcgaacc gcacctcgga
     4441 catgggagcg atggagctct acgccgcctc cgtcgtcgcc tcggacccgt tccaggttgc
     4501 ggaagtcctg cgccacgggt agctacgggt aactatctcg cgtccaccac ggcgcagacc
     4561 atcagctcga cggcccgttc atcgtgcagg ccctgcttaa gaagcaccag acgcacaacc
     4621 ccgcagtcga ggtaacggcc cggaacatcc tcccatccgc atcaccatac ttgacagctc
     4681 tgttatttta atgttagtgt taattaacat gaaaaaagac ccccaaaacc tggacaccac
     4741 taacaagcgt ccctatcgga tgggggcgcg ggcggaggcg gcggaggaga ctaggcggag
     4801 gatcctgaac tcggccatcg agctttatac gggacggtac tacgatcagg tatctctcga
     4861 agacgtggcc ggacgggccg gagtcacggt gcagacggtt ctccggcgct ttggcagcaa
     4921 ggagggactc atcgacgccg cgaccgaagt gg
//
LOCUS       DASSMB010000043        33641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_263859, whole genome shotgun sequence.
ACCESSION   DASSMB010000043 DASSMB010000000
VERSION     DASSMB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 33641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33641
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263859"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFJ72_02970"
     CDS             <1..478
                     /locus_tag="VFJ72_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7478461.1"
                     /translation="TKDGDYALIAGNADTVFGRLAKALGHPEWAEDEKFATHHARGEN
                     MEELDAMISAWTRERTVDEVLGAMAEAGVPAGKVFTAEDMLEDPHYAARENVITVEDP
                     EIGEFPMQNVVPRLSETPGEVRWTGPALGQHNEEVYAEVLGLSSEELDMLRERGIV"
     gene            478..1371
                     /locus_tag="VFJ72_02975"
     CDS             478..1371
                     /locus_tag="VFJ72_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET7478462.1"
                     /translation="MRVEVVDVGPRDGLQNEDRTLTPEVRAELCDRLAAAGVSRVEAA
                     SFVNPRRVPQMAGAEEVMSGIRRSPGTSYAGLVLNERGYERAVQAGVDEVRYAFPVME
                     TFAKRNQNTTVEDATALAGRLVERARLDGVRVSITLSAAFGDPFEGRVDPARVLRIAE
                     VVAEARPDEVVLADTIGVGVPSQVHQLVEGVAGFGVTVGCHFHDTRNTGIANAAAAIE
                     SGATVLDASVGGTGGCPFAPRATGNIATEDLVYLLHGMGHETGIDLEGLIEVAAWLAE
                     QLGKELPGQVYKAGNFEPVAG"
     gene            1429..3483
                     /locus_tag="VFJ72_02980"
     CDS             1429..3483
                     /locus_tag="VFJ72_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET7478463.1"
                     /translation="MAYNVAVDVGGTFTDVLVFNEDTGELTEGKVLSTPEDPSKGVVT
                     GIESVCGKVGIGFTDLNLLFHGTTVVTNMILTNTGSRVGLLTTKGHEQILHLARAWTP
                     GPLYGWMALQKPDPPADPTDTIGISERITAGGQVLRELDEDEVRQAVRKLVDRGVESL
                     AIGFLNSYVNPAHERRARDIVREAHPDLSVSISADIGQEYGEYERTLTTVVNTAVQPR
                     TIVYMRNFESSIAEKQFGGALSIVRSDGGAMSTEAVAARPIQIALSGPSGGVTGSAYL
                     AREMGAPDILTFDMGGTSTDVSLCLGGEPLVRREIQLGYYQFKSPAVDVHSVGAGGGS
                     IAFVSKSGALQVGPASAGADPGPACYGRGGEEPTVTDANVVLHRIPPGVALGGTLDLD
                     EDAAKKAVGKIAESVGMGVEEAAEAILDIVNENMHAALRVVSVERGYDPRNFGLVAFG
                     GAGPMHANALGRLIRSRSVIVPPTPGVMSAFGFLSSDIQNEFPETYLKIAEETPAQEM
                     KQTFESLIAQADEWLDGEGVDEDKKRFDLYADCRYYLQNIQIPCRFDLDELKDGDSAF
                     LRERFEEAHRQRYNFELPDSPLEIATIRVVGRGTIRGVSLVESEDGAGKDASGAVIRE
                     DRVFFNGEWMETPIYERGKLRPGNVISGPAVVAQDDTTTVIEPGYRGAVDRYGNILIE
                     EA"
     gene            3488..5380
                     /locus_tag="VFJ72_02985"
     CDS             3488..5380
                     /locus_tag="VFJ72_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET7478464.1"
                     /translation="MIHKEALPDFVREHDIDKVTLDIIENALKNIRDEMDRVLVTTAV
                     SPIIREQADEFPLIADKKGRMIVGQFGSPVDTVLENSPYKVEDLKDGDLIALNDPYMM
                     EGSTSHLPDILLVRPIFYDGDHIGYALQWGNLMDVGGKTPGSIPIDARSIYEEGVRMP
                     PVKLYDGGKLNEEVLRFFCHNSRTPRETRADIMAIAAGTAAGAQRVKDICDRFGKDTY
                     LEACDALLDRTRTSVMGLIQQLIPEDQRFEFEDYTDDDGLGNGPIKLKLAMWREGDSL
                     HLDWTGTDPQVPGPVNFLLNHEMFQMFAGVFLISAVDPTILFNDGYADIIKVTIPDGS
                     VLRPKAPAPLSNRLVVMARLFDVLGAVYAKAIGFNVSGSYGTSPNFVYSGVKESGETF
                     QTMEILYGGIPAIPGKDGLDGHSWWPEFLAVPAEYMETYYPMIVEEYATRRDSCGAGQ
                     FRGGCGIKKVYRFLADGSITYQDDRAATFPYGVAGGKPGASSRKTLIREGGEEVDLPS
                     KVSGVPVYEGDLLVFETAGAGGVGDPLDRDPEAVAKDVRWNLISREAAENDYGVVLDG
                     EGAIDTAATESRREELKSSRGELPQFDHGPLPSLEEQRIAIAETRRKFNEWLSGEMGS
                     ARRNGR"
     gene            5385..6011
                     /locus_tag="VFJ72_02990"
     CDS             5385..6011
                     /locus_tag="VFJ72_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7478465.1"
                     /translation="MTHIQGFGERGGFGRRPALAVIDMTLGFTDPESPLACDLEGPVA
                     EIQKILEAARRAEIPVIFTTIAYRESDKLTAAAFIDKVPALLTLEAGSRWADIDPRIA
                     PRESEPVLNKLFASGFFGTGLSSALTAAGVDTLIITGASTSGCVRATAVDALQYGFRP
                     VVPREAVGDRNPDAHEANLYDIDAKYGDVTTVKDTLAYLEEIKAATAL"
     gene            complement(6041..6625)
                     /locus_tag="VFJ72_02995"
     CDS             complement(6041..6625)
                     /locus_tag="VFJ72_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET7478466.1"
                     /translation="MSNPPEHVDTVPGEDPPVTAVASRRVKPGREQEFEEWVSGILGA
                     ASEYPGYLGSNIIRPSDSDDDEFQIVFKFDHVSNLKKWERSEERHLWLRKSRDLLLEQ
                     EKVRILTGLETWFTLPAKAGEPSPPRYKMAIVTWLAVFPTVLVIFTIFGPLLNLLPMA
                     LRTLVFTLTMVSLMTYVIMPRMTRLFSFWLYPKD"
     gene            6860..8194
                     /locus_tag="VFJ72_03000"
     CDS             6860..8194
                     /locus_tag="VFJ72_03000"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7478467.1"
                     /translation="MTEKSESMDGKGTATQGDSSLSITDNRTGKSYDVEITDGTVRAM
                     DLRQIKVDDEDFGLMTYDPGFTNTASCRSAVTYIDGEKGILQHRGIPIEQLCEKSTYL
                     EVAYLLIYGHLPTEDELADWVHQITHHTYVHENIKNFMNGFRHDAHPMGMLLASVGAL
                     STFYPEAKDIDDEYQQHVTAVRLIAKVPTLAAFSYRHSLGLPYVYPDNDLSYVGNILS
                     MLFKMAEPKYEPDPRLEKALDVLFILHADHEQNCSAAAVRVAGSSRVDPYSAIAAGVA
                     GLYGPLHGGANVDVLQMLKRIGSTDNILDFLQGVKDRKEKLMGFGHRVYKNYDPRARI
                     IRSHVDEVLEATSKESPLLDIAMELEKRALDDDYFTSRKLYPNVDFYSGLIYEALGIP
                     TNAFTVMFALARTSGWVAQWMEMLRDPELKIARPRQIYTGALDTDYTPIGER"
     gene            complement(8238..9731)
                     /locus_tag="VFJ72_03005"
     CDS             complement(8238..9731)
                     /locus_tag="VFJ72_03005"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET7478468.1"
                     /translation="MKGGDSGKTNVLALDLGSSSVRAALYDGAGAPVDGTEVKLDYDL
                     DYTRDGGATKSAEEVLDLLTRAIDGALSKAGGASVSCVGASTFWHSVTGLDQAGNPVT
                     PILTWADRRSAAEAADLRMRLDESAVHRRTGCVLHSSYLPAKLLWLSRNHPEQFARAE
                     RFVSVGEYLYEQLFGGDAVTVGTSMASATGLFDQHRLVWDEGMLDALPIDEAQLSPIS
                     DEPASGLTAEYAERWPALADVPWFPAVGDGACSNAGSGCVTEDRLALMVGTSGALRVL
                     WKAGSVEIPDGPWCYRADAERFVMGGALSNGGNLVQWLRDTLRLPEPGEIDKQLSRME
                     PDSHGLTFLPLLAGERGPAWADHANGTIAGLSMTSGPVEIMRAAMESVALRFAIIAEQ
                     LESTSPGEKEIVATGGGLLNSPTWTRIMADVLGRPVTLSGVKEASSRGAALLALEKLG
                     TLKIEDIEAPLGDTFDPDPERHKVYRNALERQRRLYDAVMRSSGTGE"
     gene            9873..10775
                     /locus_tag="VFJ72_03010"
     CDS             9873..10775
                     /locus_tag="VFJ72_03010"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7478469.1"
                     /translation="MASDERLRELEEKYEGYKVYDNRGEKIGKVDDLFIDESDREEYI
                     GVKMGLFGTKSTLIPMDIVRVNESDRTIEVSESKDRVKDAPNFSDDDDITPDYEDRIR
                     SHFGLESLGDTSSRGSYGSYGAAGAASAGRGVTDRDRGDYDDRDDRDRDSRDISGSGE
                     RGYDERRDRDTGAASRVDTEYGERAGSSERGSEQDRGASSSRDDDSISSTTSGDREGH
                     SEYRGSEDLRRESSEPRTFGSGGSSDEDRQSESGGSFGESETREMPMNRQTEETETFQ
                     EGGRTKVRRRIIREEIIEEDPDQR"
     gene            complement(10845..11300)
                     /locus_tag="VFJ72_03015"
     CDS             complement(10845..11300)
                     /locus_tag="VFJ72_03015"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HET7478470.1"
                     /translation="MLRVFVYGTLKRGQRNHDFYCQGVEEISEAITVGQLFDLPFGFP
                     GLRVPVQSVHATGSADYCADALRNGPAVRSMPDAAHPWGPVHGELMAFPDPASLSRLD
                     ALEGYTPGEKGLYERVLVPVEAHDETRLAWAYAITREIGSRLPGGRWPA"
     gene            complement(11319..11738)
                     /locus_tag="VFJ72_03020"
     CDS             complement(11319..11738)
                     /locus_tag="VFJ72_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7478471.1"
                     /translation="MHQNPSDEEIRELLQSSRTVAVVGLSDRPYRTSHAIARALQSFG
                     FRIFAVNPNLTAPVLGEQPHASVEEIGAPVDIVDVFRSSEKVMPTAVDAVAAGARALW
                     MQSGVINEEAAAYAADHGLTVVMDRCIKVEYAALAAR"
     gene            complement(11869..12138)
                     /locus_tag="VFJ72_03025"
     CDS             complement(11869..12138)
                     /locus_tag="VFJ72_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478472.1"
                     /translation="MIRTGYPTQNLSVPASTNRTLCLTNLSNTGKVRGISRQNVAEMR
                     EALKLTLPTWDVRGARQKVHLSTQDPRPSHPALTPTPSPSKTGAP"
     gene            12319..13617
                     /locus_tag="VFJ72_03030"
     CDS             12319..13617
                     /locus_tag="VFJ72_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET7478473.1"
                     /translation="MRENDMGGWTRASQDLYPHQWSWDTGFIAIGLAHLDVARAAREL
                     LGLFDHQWKTGKVPHIVFNPDAPPGSYFPGAEHWSSAGKFPDAPPSPPYTSALCQPPI
                     HAIAAWRIWEVAVRAGQEAIAIQFLREIYPRLLRWHRYLLTYRDPEGSGLVTIYHPWE
                     SGTDNSPRWDGAMQNLEVGEVPDYERRDLQHVEDPEARPTGFEYDRYIWLVELIKRAR
                     CDERVLYATHPFLVKDVLASAILIAANEALLDISGLVDAAESDRAEMIGWIDRGRRSL
                     DSCWDGEIGLTLDFDLREGSPLRSRTVAGFSALLDRHVAPERIDRQVETLFSERFAGN
                     PRLRWPLPPSASPGEEGFQPRSYWRGPTWPMIHWLLWWTLTRIGETRAAANLRQAALT
                     QLAEEGFAEYFEPFTGVPLGSLRQSWTAAVALDWLADDST"
     gene            complement(13684..13902)
                     /locus_tag="VFJ72_03035"
     CDS             complement(13684..13902)
                     /locus_tag="VFJ72_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HET7478474.1"
                     /translation="MSVARVIEISAVSQDSFEDAVRQGVTRASSTLRGVQSAWVKDMN
                     VMLDNGNITGYKVNLEVTFLLEDSQSLS"
     gene            complement(14023..14787)
                     /locus_tag="VFJ72_03040"
     CDS             complement(14023..14787)
                     /locus_tag="VFJ72_03040"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7478475.1"
                     /translation="MDRTRFDELKESYVLSALSDDERRGFEEYLRDHPECQAEIDELN
                     GLAGLLALSTPEMEPSPELRRNLMSVVESEARQMRSATRSEERRTSLGWISNLFTGQR
                     LVLGAAALAIIGLLSWNFVLQGEIKDLNGQVAEARNTGAQTAGTAGTAQADTVELKGS
                     AATQDARVELVRLGEKRTILVAQNLPPIEQGKTYQLWIIDNDTPKPSGLFKTGKNLIS
                     TPVKNSLKGADMVAITVEPAGGSQAPTSTPMMSAKL"
     gene            complement(14802..15368)
                     /locus_tag="VFJ72_03045"
     CDS             complement(14802..15368)
                     /locus_tag="VFJ72_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7478476.1"
                     /translation="MLTLADEDLIALAEGGDSEAFAALYDRHSRAAYSLAYRMMGEKQ
                     AAEDLVQDVFLKVWRSAGSYRVERGSVRTWILSVLHNRGIDQIRSSASRRRTQDKIEA
                     SAPKSQPSEAFAETWRNTQRDQVRDALTSLPPEQLKILELAYFSGYTHVEIAEILDLP
                     LGTVKGRMRLGLKKIKEYFNSRDIAVPG"
     gene            15627..16292
                     /locus_tag="VFJ72_03050"
     CDS             15627..16292
                     /locus_tag="VFJ72_03050"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7478477.1"
                     /translation="MSEAESRLAPVGIDREYSRRNFLKTAAAVGTAISAAGVVGFGVA
                     ERYANAADQPYKSYEGLGDVAILQFAYLLEQLEGVFYQTGADAGIFDGNTLAQIAAIR
                     DHEIAHAEAIAATLGQLGAEVPATPQFTFPDGVFGDPAAFLKLAATFEPVGIGAYQGA
                     APALESKDILAAAISIHNAECQHRCAINILSGVNPPNDKAFEDALPLQKVQDAVKPFG
                     ITG"
     gene            16309..17001
                     /locus_tag="VFJ72_03055"
     CDS             16309..17001
                     /locus_tag="VFJ72_03055"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7478478.1"
                     /translation="MDKHTINVPGVEAFAQPQSRKNFFKALAFAAAGTAVGSTVLTRK
                     AYAQSGNGDVDIANFALTLEYLEAEFYANAVDAGVLSGDAAGVVKNLADHEQQHVDAI
                     TGLLKSAGAKPVAKPKFTFPADAFSSQDSILNLAATFEPVGVGAYLGAATMIQSPDIL
                     AAAGSIAGVEGEHVVAVNNLLGVVPPANQAMPASLTKDEVLAAVAPFLGMGGMMDTGG
                     VEYRDRNSFGRV"
     gene            17069..17896
                     /locus_tag="VFJ72_03060"
     CDS             17069..17896
                     /locus_tag="VFJ72_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET7478479.1"
                     /translation="MALLLLFALLSVTGCNASATQSDTASSNHEATSGLETSSQTEET
                     TAGAQDTGKKSAHSEGDRKNSDGEAKQEPQKTRKPIEGKVSKDALKDVPKFRDFYSEG
                     NPKTPKNLVKNGSTAGAIPAVKPFNFGRDPGGPDDKTLYLSIPKIDRYDVPVYNSTSE
                     DDLTRSAVHVPATGFPWQSGANTFIAGHRLGYPNTGSYYVFYRLNELVAGDEISVRDS
                     DGRSFVYRVTDQKTVGPENIDVMNPVEGKSIISLQTCTLPDYSERLIVQGELVEKDV"
     gene            17978..18793
                     /locus_tag="VFJ72_03065"
     CDS             17978..18793
                     /locus_tag="VFJ72_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7478480.1"
                     /translation="MRLSVLTSGSSGNSTYIESKASGILIDAGLSCRRIRSLLARIGR
                     GLEDVSAILLTHGHSDHTSGVRSLLRERDLPVFAAPGVGEQLGAVIVSPGEPLDVCGF
                     EARFFSVPHDAETYGLRLTDGESCGVLATDLGEVGPEALRWMRGADVAVLEANHDPEW
                     LRRGPYSADLKRRISSSNGHLSNYQAAEVAVDLASHGLKDLVLAHLSRTNNSPARACG
                     TVSRALREAGYGEVRVRAALAGHPTPEIEVGTPMAGPEYVYRYGAGQQLFGME"
     gene            18797..19270
                     /locus_tag="VFJ72_03070"
     CDS             18797..19270
                     /locus_tag="VFJ72_03070"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET7478481.1"
                     /translation="MIRRATIVAVGKVKGWAADGCEDYAGRISRHFPVRIIEVPEEDM
                     NRRGRDEVLLAEAARLTRSAPSGTHVVALDRERGKQLSSEKVSSRLESLGSEGRSHVV
                     FLLGGPLGLAPEMVERADEVWSFGAVTLPHALARVVLLEQLYRAVKIGRGEKYHW"
     gene            19334..19567
                     /locus_tag="VFJ72_03075"
     CDS             19334..19567
                     /locus_tag="VFJ72_03075"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HET7478482.1"
                     /translation="MTYMRVQIDRFEDRGWAVLLLYPEGKRSFDVPREMLPDGAREGD
                     VFEARFEPVPEERERLARENRQLVDELLKRDEK"
     gene            19564..21201
                     /gene="argS"
                     /locus_tag="VFJ72_03080"
     CDS             19564..21201
                     /gene="argS"
                     /locus_tag="VFJ72_03080"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET7478483.1"
                     /translation="MSFEERISTAVREAASAAFGVELGAVHVERPNEAAHGDFATNVA
                     LANARVFRRNPREVAEKLAETLDAPFVERFQIAGPGFINFWLSPVALWEELARLRRDG
                     KNYLRGGESGETVLLEFVSVNPTGPMTVAHGRHAAYGDSMARIMEAAGQEVTREYYFN
                     DGGNQIRLLGESVAVRYAELHGREWSVSDPDALYQGEYIRDIARDFSRVRGEAYLEMD
                     SDKALAEISAFAVDWCMEDIKKTLGRARVVFDSYFNEKSLYESGAIEELLRDLEQGGH
                     VYESEGALWLDSSKFGDDKDRVLVKRDGSYTYMAPDVAYHRDKWNRGYRTAVDVLGAD
                     HAGYPPRIRAGLQALGIPGEFLDVELIRLVKLLRGGEQVKVSKRAGTFVTFDELLDEV
                     GEDVARYFYVRSSHKTEMNFDLDLAIQQSDENPVFYVQYAHARIASIFGRAGISPDDL
                     EETTTGDLAPEERALMMELLDFPRVVRNAAARREVHPVPTYLETVATRFHQFYTVHRV
                     LVDDEAVRERRLSLCAATKTVLAAGLNLLGVSAPEKM"
     gene            21198..21863
                     /locus_tag="VFJ72_03085"
     CDS             21198..21863
                     /locus_tag="VFJ72_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478484.1"
                     /translation="MKVRLPAAVWKPCLALLQTAIILQAAGCQPTELVASAVETHVKA
                     DGGAERRVILDASPPGPKLSLAPAWSIREGLDPKGRSVSRGATFEDLAGPGRAGMRLD
                     VKDRFLWREYAFSDRPGGLLEAGRELSGKTGYKLVMPGRITESPGASQRTGAVALYRF
                     ERDERVEVKAKSWAVRWWAVAVTAAGVAVAGALVFGGRAGAVARGFGRWLWTGQVRRE
                     GQG"
     gene            21922..22746
                     /locus_tag="VFJ72_03090"
     CDS             21922..22746
                     /locus_tag="VFJ72_03090"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7478485.1"
                     /translation="MGKAGKLTICQISDIHCGSGYFIPDLLERSILEINDMEPTAVVV
                     SGDLTDAGYRQEFETSAEYVRRFRCEHVMVIPGNHDSRNVGYVHFERLFGERYSCIDF
                     EEAVMVGIDSSEPDLNDGRVGREHYQFIHDSFAGKDDKLKIFVIHHHLIPIPGTGRER
                     NIIFDAGDVLELLDDVGVDLVLSGHKHVPYSWKLENMFIVNAGTASTTRLRGNTRPCY
                     NIIEIEDGQVRVFRKYPFKERELTVDFNSVTHEYSRYEERIDQEVTPDPEIAVSGD"
     gene            22756..24087
                     /locus_tag="VFJ72_03095"
     CDS             22756..24087
                     /locus_tag="VFJ72_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HET7478486.1"
                     /translation="MRALFLIDGEHYPPVVLDAMRSVRESMGAEGVAAAFLGGTEKIR
                     EGTDYGVPLVSGEDPISAVRRALSEHSVDVVVDLSDEPVIGYRERMRIASLSLAAGAR
                     YVGSDFELRPPELRDVSTKPSLAVIGTGKRVGKTAISGYLARLLAREDYAPGVVSMGR
                     GGPREPEVIHGHEMEVGSDYLLEALERGAHAASDYYETAALSRVITVGCRRCGGGLAG
                     EPFVSNVLEGARIANGLDTRITVFDGSGAAMPPVAVGRRVLVAGAHQDPEYIAGFLGA
                     YRLLLSDLLLLTMSEEPMASPAKVREILEAVAGVRPDLRVIPTVFRPRPVGDIEGLRV
                     AYVSTAPPAVLDKLSRHLEEQYGCEVVAASGNLSDRRLLARDLDAVKDADAYLTEIKA
                     AAVDMVTRRGSAEGKPVLYCDNDPVGDDLDGTLLKIARSAVEEYTASRTAD"
     gene            24558..26087
                     /gene="rho"
                     /locus_tag="VFJ72_03100"
     CDS             24558..26087
                     /gene="rho"
                     /locus_tag="VFJ72_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HET7478487.1"
                     /translation="MTELATLERKRLSDLHQIASQLGIEQYRKYRKPELAEMIYKVAT
                     EQAGAQSATVSAAPPVVPTPAEPPVPEQPAATEPARIETPSNGNGSGETDGGVAVADE
                     VGQRRQEQRGRDKEARQRQDRRPDRSRDERGREDRGEQPTQSGILDVLPDGFGFLRTS
                     GYSQSKGDVYVSTSQIKRFNLRRGDYVVGQIRPAREGEKYPALVRIETVNGEEPQKGK
                     RRQNFDDLTPLYPQERLRLEWKPNDIAPRVIDLVAPIGKGQRGLIVSPPKAGKTTIMQ
                     QIAQSIASNYPDVKLMVLLADERPEEVTDWQRSIPEAEVIASTFDQPAENHVAVAELV
                     LERVKRLVEEGEDVVVLLDGITRLSRAYNLAAPASGRILSGGVDSAALYPPKKFFGAA
                     RNIENGGSLTILATALIETGSRMDEVIFEEFKGTGNMELRLDRKLANRRIYPAINIED
                     SSTRKEELLMEAGEAQRVWQVRSILNALESSQKIELLIQKLKETRTNAEFLRELQRVR
                     G"
     gene            26194..26409
                     /gene="rpmE"
                     /locus_tag="VFJ72_03105"
     CDS             26194..26409
                     /gene="rpmE"
                     /locus_tag="VFJ72_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HET7478488.1"
                     /translation="MKTGIHPEYTTTLVHCGCGNEFETRSTAGGTITVDVCSVCHPFY
                     TGKQRIVDTGGRVQRFENRRQARQNRK"
     gene            26421..27335
                     /locus_tag="VFJ72_03110"
     CDS             26421..27335
                     /locus_tag="VFJ72_03110"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET7478489.1"
                     /translation="MRVGGQAIMEGVLMRSPNFWGMAVRTPSGDVDIRAEQFRSVTRR
                     NKLFRLPIVRGALSLGETLWLGMKALTLSTNIALGEEEDLSKKEIAGTLLFGLVLGFL
                     LFLAAPVFGTKGLGALLGNSIENPVLFNLIEGTLRIGFFMLYLVGITAISKDIKRFFA
                     YHGAEHKAIKVFERGEELVPENARKLDTSHVRCGTSFVLYVLVLSILAFSLLGVEGWF
                     YMLASRVVVIPLVAGIAFEFIMWSAKHQDSSIVRALTWPGLQLQKLTTREPDDSMVEV
                     AMASLKKVLSMENEAKIAPNEATNKLVI"
     gene            27352..28452
                     /gene="prfA"
                     /locus_tag="VFJ72_03115"
     CDS             27352..28452
                     /gene="prfA"
                     /locus_tag="VFJ72_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET7478490.1"
                     /translation="MKLAEETLGRYNALTEELSDPNIFNDQNRYREVAKEHARMKRGA
                     EISQGLLDSIEEEREARELIPTAADAEEREFFTAEARAAEERIEELSEKIRAELIDRD
                     PNDDKDVIIEIRAGTGGDEAALFAGELLDMYTRYAERLRFKHRTLSSSPAEVGGYKEV
                     AVEIEGDRAYSVFKHEGGTHRVQRVPKTESQGRIHTSTATVAVLPEAEDVEVEVNQGD
                     LEIDVYRSSGPGGQSVNTTDSAVRITHKPTGLVVTCQNEKSQLQNKEQAMKILKSRLL
                     EREMRERAEQEGAMRQAQVGSGDRSAKVRTYNFPQGRITDHRVGVTSHNLEATLGGDL
                     EEFTQALAAKERSDKLAAEANGSGPSGNGASG"
     gene            28456..29322
                     /gene="prmC"
                     /locus_tag="VFJ72_03120"
     CDS             28456..29322
                     /gene="prmC"
                     /locus_tag="VFJ72_03120"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7478491.1"
                     /translation="MRTGAVSARELSREAADRLRQAGVPEPDASAEVLLSELLGTGRG
                     ELVLYERPLDEGQRALYEAWISRRIQREPVQRILGYAYFRNIKLELDEHTLIPRPDTE
                     SVAEAALQAIDARGGSCRVLDIGTGSGAIAISVAQERPRCDAYASDISERAVTLARRN
                     AEAAGAQVHFLQADVGFGLEGLAGSIDVLVSNPPYVRSGDIRGLAPEVRDWEPHAALD
                     GGPDGLRFYRRIFTEAPFLLVPGADVVVEVGDDQAEAVLGLGREAGFVPVGVGRDLAG
                     SPRIALLRWDGV"
     gene            29319..29888
                     /locus_tag="VFJ72_03125"
     CDS             29319..29888
                     /locus_tag="VFJ72_03125"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET7478492.1"
                     /translation="MKYVAVREAADVLRRGGVALVPTETVVGLVAADSGFSRLWEIKG
                     RDAGKPIALLCESADQAFSLARETPPLARALAAAYWPGPLTLVLDAPEGGTVGVRVPD
                     GSAVRELLAVYGGPLYATSANISGGPDVRNIEQVDPVVASAVDVAVRGEPGTGEASAV
                     VDLSSGQVRLLRPTEDLSEARLRRFAELE"
     gene            30061..31284
                     /gene="glyA"
                     /locus_tag="VFJ72_03130"
     CDS             30061..31284
                     /gene="glyA"
                     /locus_tag="VFJ72_03130"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7478493.1"
                     /translation="MIGPYTEVVDSEVQDALDGELQRQRTTIELIASENFASPAVLAA
                     QGSVLTNKYAEGYPGKRYYGGCEQVDKVEVLAIERAKQLFGADHINVQPHSGSQANEA
                     AYAALIDPGDEVLSMDLAHGGHLTHGMKINFSGRTYDFAHYGVGEDGYIDYDQVRDIA
                     LRHKPRLILAGASAYPRVIDFEKFREISDEVGAFFLTDMAHIAGLVAADVHPSPIPHC
                     EIVTTTTHKTLRGARGGMILCREEFAKKVNSRVFPGMQGGPLMHAIAGKAVAFGEALQ
                     PEFKDYARRIVDNCRAMAEGLLEGGINLVSGGTDNHLILVDLRGSGTNGKELEEKLES
                     IGVTCNKNMVPNDPEPPTVTSGVRLGTAAMTTRGMGEDEMREISDIIVRAARGEVEGL
                     RDRVASLTDAFPLYE"
     gene            31277..31924
                     /gene="upp"
                     /locus_tag="VFJ72_03135"
     CDS             31277..31924
                     /gene="upp"
                     /locus_tag="VFJ72_03135"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET7478494.1"
                     /translation="MSETKPLAENFHLVDGPLSRHKLAVLRDEKTRTPDFRQAMKELA
                     LIMVAEATRDMPTRTASIRTPLQETEVEEISGPVCLVPVLRAGLGMLDGALALLPTAT
                     VGFMGLFRDEETALPVEYYVNLPTNLEDYLVLVLDPMLATGGSLSATLDKLKEYGATW
                     ISCLHAVSAVPGVERVTREHPDVQFYTAALDPELNDAAFIVPGLGDAGDRLYGTL"
     gene            31935..32411
                     /locus_tag="VFJ72_03140"
     CDS             31935..32411
                     /locus_tag="VFJ72_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET7478495.1"
                     /translation="MDTTNEHDTLKKVRPSWDEYFMRIAHEVATRSTCPRLAVGAVIV
                     RDKRILTTGYNGSPSGLPHCDDVGCLIRIVDGRESCQRTLHAEQNAIIQAAYHGVSVT
                     GASIYSTHQPCLMCVKMIMNAGIQEVHYAGGYPDPLAMDLATEGGLTMVKFAPDEN"
     gene            complement(32416..33051)
                     /locus_tag="VFJ72_03145"
     CDS             complement(32416..33051)
                     /locus_tag="VFJ72_03145"
                     /inference="COORDINATES: protein motif:HMM:NF044444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7478496.1"
                     /translation="MSGRPRRRHTSQQAERAILAAARSFLAEHPFRELTAEAVMTRTG
                     LSRTAFYAYFRDRYDLVTRLLEGIGALLFAVDRYWLEGGEEAGAPASESLGEALRRGA
                     ETFVEYGPVLRAIADAASHDSRVEQVYRYGLIENLIQAVADRVSRDVEAGISSGEIDP
                     EETARALVLMTERYLLDAFGRPQAKPSEERIAIVVGTLERVWELTLYGPEA"
     gene            33184..>33641
                     /locus_tag="VFJ72_03150"
     CDS             33184..>33641
                     /locus_tag="VFJ72_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7478497.1"
                     /translation="MAVARVSGGMREVRLEHGVIRYREAGSGPALVFVHGILVNGTLW
                     RHVVARLSEKFRCIVPDLPLGSNSIPLGADADLSPEDVARIVADLIETLNLEDVTLIG
                     NDTGGAICQIVIAEHSDKVSRLVLTNCDAYEAFFPLLLRPFQYAARIFGVR"
BASE COUNT         6256 a        10457 c        11229 g         5699 t
ORIGIN      
        1 gacgaaggac ggcgactacg ccctgatagc cggcaacgcc gacaccgtct tcgggcggct
       61 ggcgaaggcg ctcggccatc ccgagtgggc ggaagacgag aagttcgcca cgcaccatgc
      121 gcggggcgag aacatggagg agttggacgc catgatctcc gcctggacca gagagcgtac
      181 ggtggacgag gtgctcggcg ccatggccga ggctggagta ccggcgggga aggtcttcac
      241 cgccgaggat atgctcgaag acccgcacta cgccgcccgc gagaacgtca tcaccgtcga
      301 ggatccggag atcggcgagt tcccgatgca gaacgtcgta ccgcgcctct cggagacccc
      361 cggagaggta cgctggaccg gccccgccct cggccagcac aacgaggaag tgtatgcgga
      421 agtcctgggt ctgagcagcg aagagcttga catgttgcgc gagcgaggga tcgtctgatg
      481 cgggtcgagg tcgtggacgt tgggccccgc gacggactcc agaatgaaga caggacgctg
      541 acgccggagg tccgggccga gttgtgcgac cggctggcgg cggctggcgt gtcgcgtgtg
      601 gaggcggcca gcttcgtcaa ccccaggcgc gtcccccaga tggcaggcgc cgaggaggtc
      661 atgtccggca tacgccggag tcccggaacg agctatgccg ggctcgtcct gaacgagagg
      721 ggctacgagc gggccgtaca ggccggggtg gacgaggtgc gctatgcctt tccggtgatg
      781 gaaacctttg cgaagcgcaa ccagaacacg accgtggagg atgctactgc tctggcggga
      841 cggctggtgg agcgcgcgcg gctggacggc gtgcgggtct cgatcacgct ctcggccgcg
      901 ttcggagacc ctttcgaggg gagagtggac ccggcgcgcg tcctgcgtat cgcggaggtg
      961 gtcgcggagg cccgtcccga cgaggtcgtg ctggccgaca ccatcggcgt cggggtgccg
     1021 agccaggtgc atcaactcgt cgagggtgtt gcgggctttg gcgtcacggt cggttgccac
     1081 ttccacgata cgcgtaacac ggggatcgca aacgcggcgg cggccataga gtccggggcg
     1141 acggtgctgg acgcttcggt aggggggacg ggcggctgcc cgttcgcgcc gcgggcgacc
     1201 ggcaacatcg ccacggagga cctcgtatac ctgctgcacg gtatgggaca cgagacgggg
     1261 attgacctgg aggggctgat tgaggtggcg gcgtggctcg cggagcaact cggaaaggag
     1321 cttccggggc aggtctacaa ggccggcaac ttcgagccgg tggccggatg agagcgggga
     1381 cccttccggc tctattttgt gcgggactga cgggaaaagg agcgcgagtt ggcatataac
     1441 gtggcggtag acgtgggcgg gacgttcacg gacgtgctgg tctttaacga ggacacgggc
     1501 gagctgacgg aagggaaggt cctctcgacc ccggaagacc cgtcgaaagg ggtcgtcacc
     1561 ggcatagaga gcgtgtgcgg gaaggtcggt atagggttca cggacctgaa tctgctgttc
     1621 cacggcacga ccgtggtgac gaacatgatc cttacgaata ccggctcgcg tgttgggctg
     1681 ttgacaacga agggacacga gcagatcctg cacctcgccc gcgcctggac gccgggtcct
     1741 ctctacggct ggatggccct ccagaagccc gaccctcccg ccgacccgac cgacaccatc
     1801 ggcatctccg agcggatcac cgccggtggg caggtgttgc gggaactgga cgaggatgag
     1861 gtgcgccagg ctgtaaggaa gcttgtggac cggggtgtgg agtcgctcgc catcggattc
     1921 ctcaactcct acgtcaaccc cgcgcacgag cgacgcgccc gggacatcgt gcgcgaggcg
     1981 cacccggatc tctccgtctc catctccgcg gatatcggcc aggagtacgg cgagtacgag
     2041 cggacgctga cgaccgtggt gaacacggcc gtgcagccgc gcaccatcgt ctacatgagg
     2101 aacttcgagt cctcgatagc ggagaagcag ttcggcggcg cgctctccat cgtccgctcc
     2161 gacggcggcg cgatgagcac ggaggcggtc gcggcgcgac cgatccagat agccctctcc
     2221 ggtccgtccg gcggcgtcac cggttcggcg tacctggccc gcgagatggg ggcgccggac
     2281 atcctgacct tcgacatggg cgggacttcg acggacgtgt cgctgtgcct gggcggcgaa
     2341 ccgctcgtgc ggcgtgagat tcagctcggc tactatcagt tcaagtctcc cgcggtggac
     2401 gtacacagcg tgggcgcggg gggcggttcc atcgccttcg tctccaagag cggcgccctg
     2461 caagttggtc ccgccagcgc gggcgccgac cccggaccgg cctgctatgg acgcggcggc
     2521 gaggagccca ccgtcaccga cgcgaatgtg gtcctgcacc gcataccgcc gggcgtcgca
     2581 cttgggggca ccctggatct tgatgaagat gcggcgaaga aagccgtggg gaagatcgcc
     2641 gagtccgtcg gtatgggcgt cgaggaggcg gcggaggcga tcctggatat cgtcaacgag
     2701 aacatgcacg ccgccctgcg cgttgtgagc gtggagcgtg gctacgaccc gcggaacttc
     2761 gggctcgtcg ccttcggcgg agccggtccg atgcacgcca atgcactcgg caggctcatc
     2821 cggtcgcggt cggtgatcgt gccgcctacg ccgggggtca tgagcgcgtt cgggttcctc
     2881 tcctcggaca tccagaacga gttcccggag acttacctga agatcgccga ggagaccccg
     2941 gcccaggaga tgaagcagac gttcgagtcc ctcatcgccc aggccgacga gtggctggac
     3001 ggtgaggggg tggatgagga caagaagcgc ttcgacctct acgccgactg ccgctactac
     3061 ctgcagaaca tccagatacc ctgccggttc gatctggacg aactcaagga cggagacagc
     3121 gcgttcctgc gcgagcggtt cgaggaggcc caccggcaga ggtacaattt cgagctgccg
     3181 gactcgccgc tggagatcgc cacgatacgg gtagtcgggc gcggcacgat ccggggcgtc
     3241 agcctcgtgg agagcgagga cggcgccggt aaggatgctt cgggggctgt catccgggaa
     3301 gaccgggtat tcttcaacgg cgagtggatg gagaccccga tctacgagcg cggaaagctc
     3361 cgaccgggca acgtgatctc gggcccggcg gtcgtcgcgc aggacgacac gacgaccgtg
     3421 atcgagcccg gctaccgggg cgcggtggac cgctacggga acatcctgat cgaggaggcg
     3481 tagagagatg atccataaag aagccctgcc ggacttcgtc cgggaacacg acatagacaa
     3541 ggttactctc gacatcatcg agaacgcgct caagaacatc cgcgacgaga tggaccgggt
     3601 gctcgtcacc acggctgtca gccccatcat ccgcgagcag gccgacgagt ttcccctcat
     3661 cgcagataag aaggggcgca tgatcgtggg ccagttcggt agccccgtgg ataccgtcct
     3721 cgaaaactcc ccctacaagg tcgaggacct aaaggacggg gacctgatcg ccctgaacga
     3781 cccctacatg atggagggct cgacctcgca cctgcccgac atcctcctgg tccgcccgat
     3841 cttctacgac ggagatcaca tcggttacgc cctgcaatgg ggcaacctga tggacgtggg
     3901 cgggaagacc cccggctcta tacccattga cgcccgctct atctatgagg aaggcgtccg
     3961 tatgccgccc gtcaagctct acgatggcgg gaagctcaac gaggaggtcc tgcgcttctt
     4021 ctgccacaat tcccgcaccc cgcgcgagac gcgggcggac atcatggcga tagccgccgg
     4081 gaccgccgcc ggagcccagc gcgtcaagga tatctgcgac cgtttcggta aggacaccta
     4141 cctcgaagca tgtgatgcct tgcttgacag gacgcgcacc agcgtgatgg gcctcattca
     4201 gcagctcatc cccgaggacc agcgcttcga gttcgaggac tacaccgacg acgacggcct
     4261 cggcaacggc cctataaaac tcaagctcgc catgtggcgc gagggcgaca gcctgcacct
     4321 cgactggacc ggcaccgacc cgcaagtccc cggcccggtc aacttcctcc tcaaccacga
     4381 gatgttccag atgttcgccg gggtcttcct catctccgcc gtggacccga ccatcctctt
     4441 caacgacggt tacgccgaca tcatcaaggt aaccatcccc gacggctccg tcctgcgccc
     4501 gaaggccccc gcgcctctct ccaaccgact cgtcgtcatg gcgcggctct tcgatgtgct
     4561 cggcgcggtc tacgccaagg ccatcggctt caacgtctcc ggttcctacg gcacgagccc
     4621 gaacttcgtc tactccggcg taaaggagag cggcgagacg ttccagacca tggagatcct
     4681 gtacggcggc atccccgcca taccgggaaa ggacggcctc gacggacatt cctggtggcc
     4741 ggagttcctg gccgtccccg ccgagtacat ggagacctac tacccgatga tcgtcgagga
     4801 gtacgccacg cgccgcgact cctgcggcgc gggccagttc cggggcggct gcggcataaa
     4861 gaaggtctat cggttcctgg ccgacggcag catcacttac caggacgacc gagccgcgac
     4921 cttcccctac ggcgtcgccg gcggcaaacc tggcgcgtcg tcccggaaga cgctcatccg
     4981 cgagggtggg gaagaggtgg acctcccctc caaggtcagc ggcgtccccg tctacgaggg
     5041 cgacctcctc gtcttcgaga ctgctggagc cggaggcgtc ggagacccgc tcgaccgtga
     5101 cccggaagcc gttgcgaagg acgtgcgctg gaacctcatc agccgggagg ccgccgaaaa
     5161 tgactacggt gtagtcctcg atggcgaagg cgcgatagat accgccgcga cagagtcccg
     5221 ccgggaagag ttgaagagca gccgcggcga gcttccgcag ttcgaccacg ggccgctccc
     5281 ctcgctggag gagcagcgga tagccatcgc cgagacccgg cgcaagttca acgagtggct
     5341 ctcgggagag atgggctcgg cgcgacgcaa cgggaggtag aacgttgacg cacatccagg
     5401 gcttcggcga gcggggcggt ttcggccggc gtcccgccct cgccgtcata gacatgaccc
     5461 tcggcttcac cgaccccgag tccccgctcg cctgcgacct ggaaggcccg gtcgcggaga
     5521 tacagaagat cctcgaagcc gcccgtcggg cggaaattcc ggtcatcttc accaccatcg
     5581 cctaccgcga gtccgacaag ctgaccgccg ccgcctttat agataaggtc ccggccctgc
     5641 tcaccctcga agccggttcc cgctgggccg atatcgaccc gcgcatagcc cctcgcgagt
     5701 cggagcccgt cctcaacaag ctcttcgctt ccggcttctt cggcaccggc ctctccagcg
     5761 ccctgaccgc cgccggggta gacaccctca tcattaccgg cgcttctact tccggctgcg
     5821 tccgcgccac cgccgtggac gccctccagt acggcttccg cccggtcgtc ccgcgcgagg
     5881 ccgtcggtga ccgcaacccc gacgcccacg aggccaacct ctatgacata gacgccaagt
     5941 acggggacgt aacgacggtg aaggatacgc tggcgtatct ggaggagatc aaggctgcca
     6001 cggctctttg agcgtacgga tcgaaacggg gcgcgaggat ctaatctttc ggatacagcc
     6061 agaaggagaa caggcgcgtc atgcgcggca tgatcacata cgtcatcagg ctgaccatgg
     6121 tgagcgtgaa gacgagcgtg cgtagggcca tcgggagcag gttgagcagc gggccgaaga
     6181 tggtgaagat gacgagcacc gttgggaaga ccgcgagcca ggtaacgatt gccatcttgt
     6241 agcgcggcgg cgatggctct ccggcctttg cgggcagcgt gaaccacgtc tccagccctg
     6301 tcaggatccg gactttctct tgctccagga gcaagtccct ggacttgcgg agccagaggt
     6361 ggcgctcctc ggaccgctcc cacttcttga ggttgcttac gtgatcgaac ttgaagacga
     6421 tctggaactc gtcgtcatcg gagtcgctgg gccggataat gtttgacccc aggtatccag
     6481 gatactcgga cgcagccccg agtatccccg agacccactc ctcgaactcc tgctcccgcc
     6541 ccggtttcac gcgccgcgaa gccaccgccg taacgggtgg gtcttctccg ggaactgtat
     6601 ctacgtgttc cggcggatta ctcaataaag cctgctccag atcgataagg cgttgcccgc
     6661 tacattaaag cccaggcgcc gcgcgttgac tacccaccat aggtatatac cagaggtcta
     6721 aatcctcgcc gcaagcccgg cgctttccgt gtggtcgctt ttgagggata atgaccgcgg
     6781 ccggaattaa agaaacgctt tacaagcata tatgtagggt ttgcatctat aatacagata
     6841 taacagaaag gggcacggtt tgacggaaaa gtcggaaagc atggacggca agggcacggc
     6901 cacgcagggc gatagcagcc tatctattac ggacaaccgc acgggcaaga gttacgacgt
     6961 ggagatcacg gacggcacgg tgcgggcgat ggacctgcgc cagatcaagg tggatgacga
     7021 agacttcggg ttgatgacct atgatccggg cttcacgaac accgctagct gccggagcgc
     7081 cgtcacgtat atagatggcg agaagggaat cctccagcac cgggggatac ccatcgagca
     7141 gttgtgcgag aagtccacgt acctcgaggt cgcgtatctg ctcatctacg ggcacttgcc
     7201 gaccgaggac gagctcgctg actgggtaca ccagatcacg caccacacct acgtccacga
     7261 gaacatcaag aacttcatga acggcttccg tcacgacgcg cacccgatgg ggatgctgct
     7321 cgcgagcgtg ggcgcgctct cgacgttcta ccccgaggcg aaggacatag acgacgagta
     7381 ccagcagcac gtaacggccg tccggctcat cgccaaggtg ccgaccctcg cggccttctc
     7441 ctaccggcac tctctcgggc tgccctacgt ctatccggac aacgacctct cctacgtcgg
     7501 taatatcctc tccatgctct tcaagatggc cgagcccaag tacgagccgg atccgcggct
     7561 ggagaaggcg cttgatgtgc tgttcatcct gcacgccgac cacgagcaga actgttccgc
     7621 ggcggcggtg cgggtagccg ggtcttcgcg ggttgacccg tattcggcga tcgcggccgg
     7681 tgtggcaggg ctctacggtc cgctgcacgg cggagctaac gtggacgtgc tccagatgct
     7741 caagagaata gggagcaccg acaacatcct ggacttcttg cagggcgtga aggaccggaa
     7801 agagaagctc atgggcttcg gacaccgggt ctacaagaac tacgatccgc gggcgcgcat
     7861 catccgcagc cacgtagacg aagtcctgga ggcgaccagc aaggagagcc cgctgctcga
     7921 tatagcgatg gagcttgaga agcgcgcgct cgacgacgac tacttcacga gccgcaagct
     7981 ctatcccaac gtggacttct actcgggcct catctacgag gcgctgggga taccgaccaa
     8041 cgcgttcacg gtcatgttcg cgctcgcccg caccagcggc tgggtcgcgc agtggatgga
     8101 gatgctgcgg gacccggagc taaagatcgc gcgtccgcgt cagatctaca ccggagccct
     8161 cgacaccgat tacacgccca tcggagaacg gtaggggctg atccaggagg ctggcgccgc
     8221 gccagcctcc gtcccggcta ttctccggtc ccgctggatc tcatcacggc gtcgtagagg
     8281 cgccgctgac gttccagggc gttccgatag accttatgcc gctcggggtc ggggtcgaat
     8341 gtatcgccaa gcggcgcttc tatatcctct atcttcaggg ttccgagctt ctcaagggcg
     8401 agcagggccg cgccgcggct cgatgcctcc ttcacaccgg agagcgtaac gggccggccg
     8461 aggacatcgg ccatgatcct ggtccaggtc ggcgagttca ggagcccgcc gcccgtcgcc
     8521 acgatctcct tctcgccggg cgacgtggac tccaactgct ccgcgatgat cgcgaaccgc
     8581 agcgccacgg actccatcgc cgcccgcatt atctcgaccg gcccgctcgt catcgagagg
     8641 cccgcgatgg tgccgttcgc gtgatccgcc catgccgggc cgcgctctcc ggccagcagc
     8701 gggaggaaag tgagaccgtg cgagtccggt tccatccgcg agagctgttt atcgatctct
     8761 cccggttccg gcaggcgcag cgtgtcccgc agccactgaa ccaggttccc gccgttggag
     8821 agcgcgccgc ccatgacgaa cctctcggcg tccgcccggt agcaccaggg accgtcgggt
     8881 atctccacgc tcccggcctt ccacagcacc cgcagcgcgc cgctcgtccc gaccatcagg
     8941 gcgagccggt cttcagttac gcaaccgctc cccgcgttcg agcacgcccc gtcacccacc
     9001 gccgggaacc acggcacgtc ggcgagcgcg ggccaacgct ccgcatactc tgccgtcagg
     9061 ccgctggccg gctcatcgga gataggcgag agttgcgctt cgtcgatggg cagcgcatcc
     9121 agcataccct cgtcccacac gagccggtgc tggtcgaaga gaccggttgc cgaggccatc
     9181 gaggtgccaa cggtcacggc atctcctccg aagagttgtt cgtaaaggta ctcgccgacg
     9241 gagacgaagc gttctgcccg cgcgaattgc tcaggatgat tacgactgag ccagaggagc
     9301 tttgccggca agtagctgga gtgcaatacg cagccggtgc gacggtggac ggcggactcg
     9361 tcgagccgca ttctgaggtc cgccgcttct gcggcggagc ggcggtccgc ccaggtgagg
     9421 atcggggtga ccgggttgcc ggcctggtcc aggccggtta cggaatgcca gaacgtgctg
     9481 gccccgacgc acgagacgga tgcgccgccg gccttcgata acgcgccgtc aatcgcgcgg
     9541 gtgaggaggt ccaggacctc ttccgcgctc ttcgtggcgc cgccgtcgcg cgtgtaatcc
     9601 aggtcgtagt ccagcttcac ctccgtgcca tctacggggg cgccggctcc atcatagagt
     9661 gcggcgcgca cggaagaaga gccgaggtcc agggccagca cgttggtctt cccgctgtct
     9721 ccacccttca tccagtctct ccgatccccg ctttccaaag ccagtttagc aggcctcatg
     9781 gcgcctcaac tgactgggcg aatcaggttt ggcgcgcgtc cgggcgtggg tacaccggac
     9841 ggtacaggtg gaccccatca ggaggagtga ttgtggcgag cgatgagaga ctccgggagc
     9901 ttgaggagaa gtacgagggc tacaaggtct acgacaaccg gggagagaag atcggcaagg
     9961 tcgatgacct gtttatagat gagagtgacc gcgaggaata catcggcgta aagatgggcc
    10021 tcttcgggac gaagtcaacc ctcataccga tggacatcgt ccgcgtgaac gaatcggaca
    10081 ggaccataga ggtctccgag tcaaaggacc gcgtaaaaga cgctcccaac ttcagcgacg
    10141 acgatgacat aacccccgat tatgaagatc gcatccgcag ccacttcggt ctggagagcc
    10201 tgggcgacac ttcctcccga ggctcctacg gctcctacgg cgcagccggg gcggcgagcg
    10261 caggacgcgg cgtgacagac cgcgaccgtg gcgactatga cgaccgcgat gaccgtgacc
    10321 gcgactcgcg ggacatcagc ggttccggtg agcgagggta cgatgaaaga cgtgaccggg
    10381 ataccggagc ggcctccagg gtggacacgg agtacgggga gcgcgcggga tcttcggaga
    10441 gaggttcgga acaggatcgc ggcgcgagct cttcgcgtga tgatgacagc ataagctcta
    10501 ccacgagcgg cgaccgcgag ggccacagcg agtatcgcgg ctcagaggac ctccgccggg
    10561 agagcagtga accacgcact ttcggtagcg gcggctcgtc tgatgaagat cggcaaagcg
    10621 agagtggcgg ttccttcggc gagagcgaga cccgagagat gccgatgaat cgccagaccg
    10681 aggagaccga gactttccag gagggcggcc gcacgaaggt ccgtcgccgc atcatccgcg
    10741 aggagatcat cgaggaagac cccgaccagc gctagtcgcg gcatccaata ttaccgtgga
    10801 gtgggcccgg cgataccggg cccactctct ttttcgggta gttgttatgc gggccagcgc
    10861 ccgccgggga gcctggagcc tatctcgcgc gtgatcgcat acgcccacgc gaggcgagtc
    10921 tcgtcatggg cctcgaccgg aacgagcacc ctttcgtaga gccctttctc acccggcgtg
    10981 taaccttcga gggcgtccag ccgggacagg ctcgcggggt ctggaaatgc catcagctcg
    11041 ccgtgtaccg gtccccaggg atgggcggcg tcgggcatgg atcttacggc ggggccgttc
    11101 ctgagcgcgt ccgcacagta gtccgccgag cccgttgcat gaacagactg taccgggacg
    11161 cgaaggccgg ggaagccgaa aggcaggtcg aagagctgtc cgacggtgat cgcctcggag
    11221 atttcctcga cgccctggca gtagaagtcg tggttgcgct gaccgcgctt gagcgtcccg
    11281 tagacgaaga cccgcagcat gggaggtgac cgccctcgct agcgtgctgc cagcgccgcg
    11341 tactcgacct tgatgcagcg gtccatgacc acggtcagac cgtggtccgc ggcgtaggcg
    11401 gcggcctctt cgtttataac ccccgactgc atccagaggg cgcgcgcccc ggcggcgacc
    11461 gcgtcaacgg cggtcggcat caccttctcg ctgctccgaa acacgtccac gatgtccacg
    11521 ggagcaccga tctcttctac cgaggcgtgc ggctgctcgc cgagcaccgg cgcggtaagg
    11581 ttcgggttga ccgcgaagat ccggaaccca aagctctgca aggcccgcgc gatggcgtga
    11641 ctggttcggt atggtctgtc agagagccca accacggcta ccgtgcgaga gctctggagc
    11701 agttctcgta tctcttcgtc gctcggattc tggtgcatga tgtctcccag taagagtacc
    11761 ggttatcggg ttaatcttac tcgacccgga agcccagcgg cacgagtgcc cgctccttgc
    11821 tcttcgcttc gactattaca tctgcgtcgc gtccgtccag cgcgaccatc acggcgctcc
    11881 agtcttcgat ggcgatggcg taggagtgag cgccgggtgg cttggtcgcg ggtcctgcgt
    11941 cgagaggtgg accttctgcc tcgcgccgcg gacatcccag gtcggcagcg tgagtttcag
    12001 cgcctcgcgc atctccgcta cgttctggcg tgagataccc cgcacctttc cggtgttgga
    12061 gaggttcgtg agacatagcg tgcggttcgt gctcgccggt acggagaggt tctgcgttgg
    12121 atagccggtc cggatcacac cgcgactcta ccgtgctcgc ttttcggaca aagtgtgcgc
    12181 tcacccaaca tgattgccag gctctggtag catgtgcggc aatatgggga gttcttaccg
    12241 ggaggatgcc gtttgagtct cgccgcgggg tctccggcgc cagaattctc ggggcttgcg
    12301 gaaggcgcgg aatccgtatt gcgagagaac gatatgggcg gctggacccg cgcctcgcag
    12361 gacctctatc ctcaccagtg gagttgggac accggattta tcgcgatcgg tctggcgcac
    12421 ctggacgtgg cgcgggcggc gcgagagttg ctggggctct tcgaccacca gtggaagacg
    12481 ggcaaggtcc cgcacatcgt attcaatccc gatgcgccgc ccggcagtta cttccccggc
    12541 gccgaacact ggtccagcgc gggtaagttc cccgacgcgc cgccctcgcc accctacacc
    12601 agcgccctgt gtcagccgcc gatccacgca atcgccgcct ggcgcatctg ggaagtcgcc
    12661 gtgcgggcgg ggcaggaagc catagcgatc cagttcttgc gcgagatcta tcccaggctg
    12721 ctgcgctggc accgctacct gctgacttac cgggacccgg aggggtccgg gttggtgacc
    12781 atctaccacc cgtgggagag cgggaccgac aactctccgc gctgggacgg cgcgatgcaa
    12841 aacctcgagg tcggagaggt gccggactac gagcgtcgcg accttcagca cgtcgaggac
    12901 ccggaggctc gtccgaccgg tttcgaatac gaccgctaca tctggcttgt cgagctgatc
    12961 aagcgcgccc gctgcgacga gcgcgtgttg tacgcgacgc acccgtttct ggtcaaggat
    13021 gtactggcga gcgccatcct gatagccgcc aatgaagcgc tgctggatat atccggcctg
    13081 gtggacgctg cggagtccga ccgggccgag atgatcggct ggatcgaccg cgggcgccgg
    13141 agtctcgata gttgctggga cggagagatc ggcctcaccc tggacttcga cctccgggaa
    13201 gggtcgccgc tgagatcccg gacggttgca gggttctccg cgctgctgga ccggcatgtt
    13261 gccccggagc ggatagaccg gcaggtggag accctgttct ccgagcgctt tgccgggaat
    13321 ccgaggctcc ggtggccgct cccgccctcg gccagccccg gcgaggaagg ctttcagccg
    13381 cgcagctact ggcggggtcc gacgtggccg atgatccact ggcttctctg gtggacgctc
    13441 acgcgtatag gcgagacccg cgccgcggcg aacctccggc aggcggccct cacgcagctc
    13501 gcggaagaag gattcgccga gtacttcgag ccgttcaccg gcgtaccgct gggttcgctg
    13561 cggcagtcct ggaccgctgc cgtcgctctc gactggctgg ccgacgattc gacatagtga
    13621 cccgcaaagc gaaacgcccc ggccacacgg ccgggacgtc tacctttatc tctgctccat
    13681 gtactagctc agggactggc tatcctccag caggaaggtg acctccagat tgaccttgta
    13741 tccggtgatg ttgccgttgt ccagcatcac gttcatgtcc ttgacccacg cgctctgtac
    13801 gccgcggagg gtgctggagg cccgggtgac gccttgcctc accgcatcct cgaaactgtc
    13861 ctgggagacc gcgctgatct cgattactct cgctacggac atagagactc ctttcttgtc
    13921 catttcctta ccccaaaagt tatttacccg gacgccgagc gtataaaccg cggcggacgc
    13981 ttgctcgcgg taagtcggat gtagaagggg accagcgtat cgtcagagtt tcgcagacat
    14041 cattggggtg ctcgtggggg cctgggagcc accggcgggt tccaccgtga tcgcgaccat
    14101 gtccgcgcct ttgagagagt tcttcacggg ggtcgaaatg aggttcttcc cggtcttgaa
    14161 gagaccgctc ggcttcggcg tgtcgttgtc tattatccag agctggtacg tcttgccctg
    14221 ctcgatgggc gggaggttct gggcgacgag gatggtgcgt ttctctccaa gccgcacaag
    14281 ctctacgcga gcgtcctgtg tggccgcaga tcccttaagc tcgacagtgt cagcctgcgc
    14341 ggtaccggcg gtcccggcgg tctgcgcgcc cgtgtttcgg gcctccgcaa cctggccgtt
    14401 gaggtccttt atctcgccct gcaatacgaa gttccaggag agaagcccga ttatggcgag
    14461 cgccgcggcg ccgaggacga gccgctggcc ggtgaagagg ttcgagatcc agccgagaga
    14521 cgtacgccgt tcctcgctcc gggttgcgga gcgcatctgg cgcgcctcgc tctccactac
    14581 ggacatcagg ttgcgacgca gttccgggga gggttccatc tccggggtcg agagagccag
    14641 tagcccggcg agaccgttca gctcgtctat ctcggcctgg cactcgggat ggtctctcag
    14701 atactcctcg aaaccgcggc gctcatcgtc ggagagggcg ctcaatacgt aggattcttt
    14761 caactcgtca aagcgtgttc ggtccatgtc tcctccctcc gtcatcccgg taccgcaatg
    14821 tcgcgggagt tgaagtactc tttgatcttc ttgaggccca gacgcatcct gcctttcacc
    14881 gtgccgagcg gcaggtcaag tatctcggcg atctcgacgt gtgtgtaccc cgagaagtat
    14941 gcgagttcca ggatcttgag ttgttcgggc ggcagacttg tgagcgcgtc gcggacctgg
    15001 tctcgttgcg tgttcctcca cgtctcggcg aacgcctcac tcggctgaga cttgggcgcc
    15061 gacgcctcta tcttgtcctg ggttctgcgg cggctggcgg aggagcggat ctggtcgatt
    15121 ccccggttat ggaggacgga gaggatccag gtccgcacgc taccgcgctc gacgcggtag
    15181 ctcccggccg atctccatac cttcaagaac acgtcttgca cgagatcctc cgctgcctgc
    15241 ttctctccca tcatcctgta cgccagcgag tacgctgccc gactgtgacg gtcgtagaga
    15301 gcggcgaacg cctcagagtc gccaccttcc gccagggcga tcagatcttc atccgccagc
    15361 gtcagcaatc gttgtgctcg tttcaccata tgtgcattgt acgagaccag gtcaatcccg
    15421 gatcattagg tgagttcggt tcctcgagac ttcgattcct cggatcaggc taccaagatg
    15481 cggggacgag tattgttgcg aaactataac gatattgtgc caaaagatgt agttttctgt
    15541 gatctgcgcg atctactgcc tcgtagagtg caattagaca tcggcgtcga gtaggcctta
    15601 aagggcctag aaagagagga aagtcgatga gtgaagcaga gagccgcctg gctccggtcg
    15661 ggatagaccg ggagtattcg cggcggaact tcctgaagac cgctgctgcg gtcgggacag
    15721 cgatctccgc tgcgggggtt gtcggtttcg gggtggcgga gcgctacgcg aacgccgcgg
    15781 atcagccgta caagagctat gaagggctcg gggacgtggc gatcctgcag ttcgcctact
    15841 tgctggagca gttggagggc gtcttctacc agaccggcgc ggacgcgggc atcttcgatg
    15901 gaaacacgct ggcgcagata gcggcgatcc gggaccacga gatagcccac gcggaggcta
    15961 tcgccgcgac gctcggacag ctcggcgcgg aggttccggc gaccccgcag ttcacgttcc
    16021 cggacggagt gttcggcgac ccggcggcgt tcttgaagct ggcggcgacc ttcgagcccg
    16081 tcggcatcgg cgcgtaccag ggcgcggctc ccgcgcttga gagcaaggac attctggcgg
    16141 cggcgatctc gatccacaac gccgagtgcc agcaccgctg cgcgatcaac atcctgagcg
    16201 gtgtcaaccc gccgaacgac aaggctttcg aggacgctct gccgctgcag aaggtccagg
    16261 atgccgtgaa gcccttcggc atcaccggtt aagggaggat agattaacgt ggataagcac
    16321 acgataaacg tgccgggcgt ggaggctttc gcccagcccc agagccggaa gaacttcttc
    16381 aaggccctgg cgttcgccgc cgctggcaca gcggtcggct ctaccgtact gacccgcaag
    16441 gcttacgcac agagcggcaa cggtgacgtg gacatcgcca acttcgccct gacgctggag
    16501 tatctggagg ccgagtttta cgcgaacgct gtcgacgcgg gcgtgctctc gggtgacgcg
    16561 gcgggcgtgg tgaagaacct cgccgaccac gagcagcagc acgtggacgc catcaccggc
    16621 ctactcaaga gcgccggagc gaagccggtc gcgaagccga agttcacctt cccggcggac
    16681 gctttctcca gccaggactc gatcctaaac ctggcggcga ctttcgagcc ggtcggtgtc
    16741 ggtgcctacc tgggcgcggc gaccatgatc cagagcccgg acatcctggc cgccgccggc
    16801 agcatcgccg gcgtcgaggg cgagcacgtc gtcgcggtga ataacctgct cggtgtcgtg
    16861 cctccggcca accaggccat gcccgcgtcg ctaactaagg acgaggtcct cgcggccgtt
    16921 gccccgttcc tgggcatggg cggcatgatg gacaccggcg gtgtcgaata ccgcgacagg
    16981 aacagcttcg ggcgcgtcta acttggtccg ggcttttaga gaactctccc tcatcaccga
    17041 gggtctctac ggaaggagca gggtcgtaat ggctctgctc cttctctttg cgctgctctc
    17101 cgtgacgggc tgtaacgcat ccgcgacgca gagcgatacg gcctctagca accacgaggc
    17161 gacctccgga cttgagacca gctcgcagac cgaagagacc acagccggag cacaggatac
    17221 cgggaagaaa tcggctcatt cggaaggcga caggaaaaac tccgacggcg aggcgaagca
    17281 agaacctcag aagaccagga agcccataga gggcaaggta tcgaaagacg ccctcaagga
    17341 cgtaccgaag ttccgggact tctactccga ggggaacccc aagacgccga agaaccttgt
    17401 aaagaacggt tcgacagcgg gcgccatacc agcggtgaag cctttcaatt tcgggcggga
    17461 ccccggcggc ccggatgaca agacgttgta tttgagcatt ccgaagatag atcgctacga
    17521 cgtgccggtc tacaactcga catccgagga cgatctaacc cggtcggcgg ttcatgtgcc
    17581 ggcgacgggg ttcccgtggc agagtggcgc taataccttt attgcgggcc atcgtctagg
    17641 atatccgaac acgggctcat attatgtctt ctaccggcta aacgagctgg tggcgggaga
    17701 tgagatctcc gtcagggact cagatggtcg ctccttcgtt taccgggtga cggaccagaa
    17761 gacggtaggt ccggagaata tagatgtcat gaacccggta gagggaaagt cgatcatctc
    17821 cctgcagacc tgcacgctgc cggactactc cgagcggctc atagtgcagg gcgaacttgt
    17881 ggaaaaggac gtttagcaga gcgaaccgca tgctatgatc tccgggagcg gatgcggcat
    17941 gcggcgctcc tgcgcacctt cacaccgaga ggcgaacttg cggctgagcg ttctgacgag
    18001 cggaagctcg ggcaactcca cgtacatcga gtccaaagcc agcggaatcc tcatagacgc
    18061 ggggctctcc tgccggcgta tccggagcct tctcgcccgt atcgggcgcg gcctcgaaga
    18121 cgtgagcgcc attctcttga cgcacggaca ttcggaccat acctccggcg tccgctccct
    18181 gctccgcgag cgcgatcttc cggtcttcgc ggctccgggc gtaggcgagc agttgggcgc
    18241 cgtcattgtc tcgcccggcg aacctctgga cgtctgcggc ttcgaggcgc gcttcttctc
    18301 cgtgccgcac gacgccgaga cttatggtct gcgcctcacc gacggcgaga gctgcggcgt
    18361 gctcgccacg gacctcgggg aggttggacc ggaggcgctt cgctggatgc ggggcgcgga
    18421 tgtggcggtc cttgaggcga accacgaccc ggagtggctc cggagaggcc cgtactcagc
    18481 ggatctgaaa cgcaggatct cttcgtcaaa cggacacctg tccaattacc aggctgcgga
    18541 ggtcgccgtg gacctcgctt cgcacggact aaaggacctt gttctggccc atctctccag
    18601 gacgaacaac tcgcccgcga gagcgtgcgg gaccgtgagt cgcgcgctgc gggaggcggg
    18661 atatggagag gtgagagtcc gggcggcact tgcggggcac ccgacgccgg agatcgaggt
    18721 cggtacgccg atggcggggc ccgagtacgt ctaccggtat ggggctgggc agcagctctt
    18781 cgggatggag tagagcgtga tccggcgcgc caccatagtc gcggtgggga aggtcaaggg
    18841 ctgggccgcg gacggctgcg aggactatgc aggaaggatc tcgcgccact tccccgtccg
    18901 tattatcgaa gtcccggagg aggacatgaa ccgcagaggc cgggacgagg tgctgctcgc
    18961 cgaggctgcg cgcctcacaa ggagcgcgcc ctctggaacg cacgtcgtcg cgctggaccg
    19021 ggagcggggg aagcaactct cctccgagaa ggtgtctagc aggttggaga gcctcggctc
    19081 tgagggcagg agccatgtgg ttttcttgct cggcgggccg ctggggctcg cgccggagat
    19141 ggtggagcgg gctgacgagg tctggtcgtt cggcgcggtg acgctcccgc acgcgctggc
    19201 gcgggtggtg ctgctagagc aactctaccg ggcggtaaag attggccgcg gagagaagta
    19261 ccactggtga aggagcgtca catcgctgcg cgcgtttggc gggactcctt tgccggagac
    19321 ccaacctgct aacatgacgt atatgcgcgt acagatagac aggttcgagg acaggggatg
    19381 ggctgttctc ctgctctacc cggaaggtaa gcgtagcttc gacgttccgc gggagatgct
    19441 cccggacgga gcgcgtgagg gagacgtgtt cgaggcgcgt ttcgagcccg tgccggagga
    19501 gagagagcgg ctggcgcggg agaaccgcca acttgtggat gaactgttga agagggacga
    19561 gaagtgagct tcgaggagag gatctccacc gccgtcaggg aggccgcgag cgcggcgttc
    19621 ggtgtcgaac ttggcgccgt ccacgtcgag cggcccaatg aggcggcgca cggcgacttc
    19681 gcgaccaacg tggcgctggc gaacgccagg gtgttccggc gcaacccgcg cgaggtagcc
    19741 gagaaactgg cggagacgct cgatgcgcct ttcgtggagc gctttcagat cgccggtccc
    19801 ggcttcataa atttctggct ctcaccggtg gcgttgtggg aggagcttgc gaggctacgg
    19861 cgcgatggaa agaactatct gcgaggcggg gagtccgggg agacggtact tctggagttc
    19921 gtgagcgtga acccgaccgg accgatgacc gtggctcatg gccgtcacgc ggcttacggg
    19981 gattcgatgg cgcgcatcat ggaggcggcg ggccaggagg ttactcggga gtactacttc
    20041 aacgacgggg gaaaccagat ccggctgctc ggcgagtccg tcgccgtacg ctacgcggag
    20101 cttcacggcc gcgagtggtc tgtctcagat ccagatgcgc tctaccaggg cgagtatatc
    20161 cgcgatatag cgcgggactt ctccagggtg cgcggcgagg cgtatcttga gatggattcg
    20221 gataaggcgc tggcggagat ctcggcgttc gccgttgact ggtgtatgga ggacatcaag
    20281 aagaccctgg gacgcgctcg cgtcgtcttc gacagttact tcaacgagaa gagtctctac
    20341 gagtccggcg ctatagaaga gcttctgcgg gacctggagc agggtggtca cgtgtacgag
    20401 agcgagggcg ctctatggct ggactcatcg aagttcgggg acgataagga ccgggtgctc
    20461 gtgaagcgtg acggctccta tacttatatg gcgccggatg tcgcctacca ccgagacaag
    20521 tggaaccggg gctacaggac cgccgtggac gtcctcggcg cggatcacgc cgggtatccg
    20581 ccgcgcatcc gggccggtct acaggcgctc ggaatccctg gagagttcct ggacgtggag
    20641 ttgatccggc tggtcaagct cctgcgcggc ggggagcagg tgaaggtgag caagcgggcc
    20701 ggtacgttcg tgaccttcga cgagttgctc gacgaggtcg gggaggacgt ggcgcgctac
    20761 ttctacgtgc gttcctcgca caagacggag atgaacttcg acctggacct cgccatccag
    20821 cagtccgacg agaacccggt cttctacgta cagtacgctc acgcccgcat agccagcatc
    20881 ttcgggcgcg ctggcataag cccggatgac ctggaagaga caaccaccgg cgacctcgcc
    20941 ccggaggagc gggcgctcat gatggagctt ctggacttcc cgcgcgtggt ccgcaacgcc
    21001 gcagcccgcc gtgaggtgca tccggtcccg acctacctgg agaccgtggc gacccggttc
    21061 catcagttct acacggtgca tcgcgtgctg gtggacgacg aggcggtacg cgagcggcgg
    21121 ctctcgctgt gcgccgcgac gaagaccgtg ctcgcggccg gtcttaacct cctgggagtc
    21181 agcgcgcccg agaagatgtg aaagtccggc ttccagccgc agtttggaag ccctgcctcg
    21241 ctctgctgca gaccgccatc attcttcagg ccgccgggtg ccaacccacc gaactcgtag
    21301 cgtccgcggt agagacccat gtaaaggccg acggcggcgc cgagcggcgc gtgatcctgg
    21361 atgccagtcc gcccgggccg aagctctcgc tcgcgcccgc gtggtccatc cgcgaagggc
    21421 tggatcctaa aggccgcagc gtatcgcgcg gcgcgacgtt cgaggacctg gcaggtccgg
    21481 ggcgcgccgg gatgcggctc gatgtgaagg accgctttct gtggagggag tacgccttct
    21541 ccgaccgtcc cggcgggctc ctggaggccg gccgcgagct ctctggcaag accggctaca
    21601 agctggtgat gccgggacgc ataaccgagt cgccgggcgc ctcgcagagg accggagcgg
    21661 tagcgctgta ccgcttcgag cgtgacgaga gggtagaagt gaaggcgaag tcctgggccg
    21721 taaggtggtg ggcggtggcg gtcacggcgg ctggggtcgc ggtggctggg gcgctggtgt
    21781 ttggcggacg cgcgggagcc gtggcgcgcg gttttggccg gtggttatgg acgggacagg
    21841 tgcgccggga gggtcagggc tagaaggcgc tctggtatac tatttgtagc tggttcaggg
    21901 cggagtagac gcggggagaa gatgggcaaa gccggcaaac taacgatctg tcagatcagc
    21961 gacatccact gcgggagcgg ctacttcata ccggacctac ttgaacgttc gatcctggag
    22021 atcaacgaca tggagccgac ggcggtcgtg gtgtcgggcg acctgacgga cgcgggatac
    22081 cggcaggagt tcgagacgtc tgccgagtac gtgcggcgct tccggtgcga acacgtgatg
    22141 gtcatccccg gcaaccatga ctctcgcaac gtcggctacg tccatttcga gcgactcttc
    22201 ggcgagcggt actcgtgcat agacttcgag gaagcggtga tggtcggcat agactcctcg
    22261 gaaccggacc tgaacgacgg gcgtgtcggg cgcgagcact accagttcat ccacgactcc
    22321 ttcgccggca aggacgacaa gctcaagatc ttcgtgatcc accaccacct gatcccgatc
    22381 cccggcaccg gacgcgagcg gaacatcatc ttcgacgcgg gagacgtgct ggagcttctt
    22441 gatgacgtgg gcgtggacct ggtcctctcg gggcacaagc acgtgccgta ctcctggaag
    22501 ctggagaaca tgttcatcgt caacgcgggg actgcgtcta cgacccggct gcggggcaac
    22561 acgcgcccct gctacaacat catcgagata gaggatgggc aggtaagggt cttccgcaag
    22621 tacccgttca aggagcggga gttgaccgtg gacttcaact ccgtgaccca tgagtactcg
    22681 cgctacgagg agcggatcga ccaggaagtc actcccgacc ccgagatcgc cgtcagcggc
    22741 gactagccgg acggcttgcg cgccctcttc cttatagacg gggagcacta cccgcccgta
    22801 gtcctggatg ccatgcggag cgtgcgcgag tccatgggag ccgagggcgt tgccgcggcc
    22861 ttcctgggcg ggaccgagaa gatccgggag ggtactgact acggcgtgcc tctcgtctct
    22921 ggagaagacc ctatctcggc cgtcagacgg gctctttcgg agcacagcgt ggacgtggtc
    22981 gtggacctct cggacgagcc ggtgatcggc tacagggagc ggatgcggat agcgtcgctc
    23041 tccctggcgg cgggagcgcg ttatgtgggg agcgacttcg agcttcggcc accggagttg
    23101 cgcgatgtct cgaccaaacc ctctctcgcc gtgatcggga cgggcaagag ggtgggaaag
    23161 acggcgatct cgggctacct ggcgcggctg ctggcgaggg aggattacgc gccgggcgtg
    23221 gtctcgatgg gtcgcggagg gccccgcgag ccggaggtca tccacggtca cgagatggag
    23281 gtcgggagcg actacctgct ggaagccctg gaacgcggcg cccacgcggc aagcgactac
    23341 tacgagacgg cggccctgag ccgggtcatc acggtcgggt gccggaggtg cggtggcggg
    23401 ctcgccgggg aacctttcgt ctcgaatgta ctggagggcg ccaggatagc gaacggcctg
    23461 gacacccgca tcacggtctt cgacggctcc ggcgcggcga tgccgccggt agcggtcggg
    23521 aggcgagtgc tcgtcgccgg agcgcaccag gacccggagt acatagccgg tttcctcggc
    23581 gcctaccggc tcttgctctc ggacctgctg ctcctcacga tgagcgagga gccgatggcg
    23641 agccccgcga aggtgcggga gatactggag gccgtcgccg gggtccggcc ggatcttcgc
    23701 gtcataccaa cggttttccg gccgcggccc gtgggagata tcgaggggct gcgggtggcc
    23761 tacgtctcga ccgcgccgcc ggcggtattg gataagctct cgcggcacct ggaagagcag
    23821 tacggctgtg aggtggtagc ggcctccggc aacctctccg accggcggct gctggcccga
    23881 gatctcgacg cggtgaagga cgcggacgcc tacctcaccg agataaaagc tgcggcagtc
    23941 gatatggtta cccgcagagg gagcgcggaa ggcaagcccg tcctgtactg cgacaacgat
    24001 cccgtcggag acgacctgga cggcacgctg ttgaagatcg caaggtccgc ggtggaggag
    24061 tacaccgcgt cgaggacggc agattaggcg cggacgcgcg gctggtgtat aatcagaaca
    24121 acgaggcgta gcccgcaggc ttcaggccct tgacgtctta caccactccg aggaaaactt
    24181 accggcgaaa gttggtttca cgctaccagt ctggtgagtg aaagagggtg gggatcgttc
    24241 atcatcgagc ccggagatag cactgtcatg gagaccgcag cgtgcctatg aggatcccat
    24301 gaggatgtaa agctttcggg ctctcaacga taattcgagc ggggggtgag gtgcttccgg
    24361 gatgggagtc gtactgagac tctgatgata tccggcaggc tgcttatggt aagattcgta
    24421 gacaccaccc acgtcctgga gcccgatgac gggcggaggg acttccagct ttgacagaac
    24481 cccgcggccg gagcgccgcg aaccccagca acgacgcgca agttttccgt gtagcagaac
    24541 aagagaggta gagaggtatg accgagttag ccaccctaga gcgcaagcgc ctgagcgacc
    24601 tgcaccagat cgcctcacag ctcggcatcg agcagtaccg aaagtaccgc aagccggagc
    24661 tggctgagat gatctataag gtcgcgaccg agcaggcggg cgcgcagagt gccaccgtga
    24721 gcgccgcgcc accggtggtg ccaacaccgg cggagccacc ggtgcccgag caaccggccg
    24781 cgactgagcc cgcgcggatc gagactccct ccaacggcaa cggttccggg gagacggacg
    24841 gcggcgtcgc ggttgccgac gaggtcggcc agcggcggca ggagcagcgc ggccgggaca
    24901 aagaagcccg ccagcggcag gaccgccgcc cggatcgcag ccgcgacgag cggggccgtg
    24961 aagaccgcgg ggaacagccg acgcagagcg ggatcctcga cgtgctgccg gacggcttcg
    25021 gtttcctgcg gacgagcgga tacagccaga gcaagggaga cgtatacgtc tcgaccagcc
    25081 agatcaaacg ctttaatctc aggcgcggag attacgtggt cggccagatc cgcccggccc
    25141 gcgaaggcga gaagtatccg gccctggtcc gcatcgagac ggtcaacggc gaggagccgc
    25201 agaagggcaa gcggcgccag aacttcgacg acctcacgcc gctgtatccg caggagcggc
    25261 tgcgcctgga atggaagccg aacgacattg ctcccagggt catagacctg gtcgcaccga
    25321 taggcaaggg gcagcgcggg ctcatcgtgt cgccgccgaa ggccggtaag acgacgataa
    25381 tgcagcagat cgcccagtcc atcgcctcga actatccaga cgtcaagctg atggtcctgc
    25441 tggccgacga acggccggag gaggtcacgg actggcagag gagcatcccc gaggccgagg
    25501 tcatcgcctc gaccttcgac cagccggcgg agaaccacgt ggcggtggcc gagctggtcc
    25561 tggagcgcgt caagcgcctc gtcgaggagg gcgaggacgt ggtggtgctg ctcgacggca
    25621 taacccggct ctcgcgggcc tacaacctcg cggctccggc cagcgggcgt atcctctccg
    25681 gcggtgtgga ttcagcggcg ctgtacccgc ccaagaagtt cttcggggcg gcccggaaca
    25741 tcgagaacgg cggatcgctg accatcctgg cgacggcgct catcgagacc ggcagccgga
    25801 tggacgaggt catcttcgag gagttcaagg gaacgggcaa catggagctc cggctcgaca
    25861 ggaagctggc gaaccgcagg atctacccgg ccatcaacat cgaggactcc tcgacgcgca
    25921 aggaagagtt gctcatggag gcgggcgagg cccagcgggt ctggcaggtg cgcagcatcc
    25981 tcaacgccct cgaatcgtcg cagaagatag agctcctgat ccagaagctc aaggagactc
    26041 ggacaaacgc ggagttcctg cgcgagttgc agcgcgttcg aggataacga cgccgagcga
    26101 ggctcgggta cgggactgat cgtgtccgag cttctctccc tttacagaag taataaacta
    26161 tggaagcaga cgtttgagaa gcggaggttt tagatgaaga ctgggataca tcccgagtac
    26221 acgacgacgc tggtgcattg cggatgcggc aacgagttcg agacgcgttc gacggctgga
    26281 ggcacgatca cggtggacgt gtgctccgtg tgtcacccgt tctacaccgg caagcagcgc
    26341 atagtcgaca ccggcggccg ggtgcagcgg ttcgagaaca ggcgtcaggc ccgccagaac
    26401 cggaagtagg gcgcccggca gtgcgggtcg gtggacaggc gataatggag ggcgtcctga
    26461 tgcgctcccc caacttctgg gggatggcgg tcaggacgcc ctccggcgac gtggacatcc
    26521 gggcggagca gttccgctcg gtcacgcgcc gcaacaagct cttccggctg ccgatcgtgc
    26581 gcggggcgct ctcgctcggc gagacgctct ggctcgggat gaaggccctc acgctctcga
    26641 ccaacatcgc gctcggagag gaagaagacc tctctaagaa ggagatcgcc ggcacgctgc
    26701 tcttcgggct cgtgctcggt tttctgctgt ttctggcggc cccggtcttc gggacgaagg
    26761 gactcggagc cctgctcggg aacagcatcg agaacccggt tctcttcaac ctcatagagg
    26821 ggacgctcag gatcgggttc ttcatgctct acctggtggg gattacggcg ataagcaagg
    26881 acatcaagcg cttcttcgcc taccacggcg cggagcacaa ggcgataaag gtgttcgagc
    26941 ggggcgagga gcttgtcccg gagaacgcgc gcaagctgga cacgagccac gtccgctgcg
    27001 gcacgagctt cgtactgtac gtgctggtgt tgagcatcct ggcgttctcg ttgctcggcg
    27061 ttgagggctg gttctacatg ctcgcgagcc gggtggtagt cattccgctg gtggcgggga
    27121 tcgctttcga gttcatcatg tggagcgcga agcaccagga cagctccata gtgcgcgccc
    27181 tgacgtggcc ggggctgcag ctccagaagc tgacgacgag ggagcccgac gacagcatgg
    27241 tcgaggtggc gatggcatcg ctcaagaagg tgctctcgat ggagaacgag gcgaagatag
    27301 cgcccaacga ggcgacgaac aagctggtga tctaggttgg acaatcaggt agtgaagctg
    27361 gccgaggaga cgctcggccg atacaacgcg ctgaccgagg agctctcgga cccgaacatc
    27421 ttcaacgacc agaaccgtta ccgggaggtc gcgaaggagc acgcgcgcat gaaacgtggg
    27481 gccgagatct ctcagggctt gctcgactcc atagaggagg agcgtgaggc gcgggagttg
    27541 atcccgaccg ccgccgatgc cgaggagcgg gagttcttca ccgccgaggc acgggcggcg
    27601 gaggagcgca tcgaggagct ttccgagaag atccgggccg agctaataga ccgcgacccg
    27661 aacgacgata aggacgtgat catcgagatc cgggccggca ccggtggcga cgaggccgct
    27721 ctcttcgcgg gcgagcttct ggatatgtac acccggtacg ccgaacgtct acgcttcaag
    27781 caccgcactc tctcctccag cccggcggag gtcggtggct acaaggaggt cgcggtggag
    27841 atagagggcg acagagccta ctcggtcttc aagcacgagg gtggaactca ccgcgtgcag
    27901 cgcgtcccga agaccgagag ccagggacgt atccacacct cgacggcgac ggtcgccgta
    27961 ctccccgagg cggaggacgt ggaggtggag gtcaaccagg gcgacctgga gatagacgtt
    28021 taccgctcca gcggtcccgg cggccagagc gtgaacacga cggactcggc agtcaggatc
    28081 acgcacaagc cgaccggact cgtggtcacg tgccagaacg agaagagcca gctccagaac
    28141 aaggagcagg cgatgaagat cctcaagtcc cgcttgctgg aacgtgagat gcgagagagg
    28201 gccgaacagg agggagcgat gcgccaggct caggtcggct ccggggatcg ctcggcgaag
    28261 gttcgtacct ataacttccc gcagggccgc atcaccgacc accgcgtagg cgtgacctct
    28321 cataacctgg aagccacgct gggaggcgac ctggaggagt tcacccaggc cctcgccgcc
    28381 aaggagcgct ccgacaagct cgccgccgag gcgaacggct ccggtccgtc cggcaacggg
    28441 gcgtccggct aacccgtgcg taccggcgct gtgagcgccc gcgagctctc ccgagaggcc
    28501 gctgataggc tgagacaggc cggagtaccc gagcccgacg catccgccga agtcctccta
    28561 tccgagttgc tcgggaccgg gcgcggagag ctggtactct acgaacggcc gctcgacgaa
    28621 ggccagcgag cgctatacga agcctggatc tcccgccgca tccagcgcga gcccgtgcag
    28681 cgcatcctcg gttatgccta cttccgaaac atcaagctgg aactggacga gcacacgctc
    28741 ataccgcgcc ccgacaccga gagcgtcgcg gaggcggcgc tacaagcgat tgatgctcgc
    28801 ggtggctcat gccgcgtcct cgacatcggc accggctccg gggctatcgc gatctccgtc
    28861 gcccaggaac gtccccgatg cgacgcgtac gcctccgata tctccgaacg cgccgtgacg
    28921 ctcgcccgcc gcaacgctga ggctgccggg gcgcaggtcc acttcctgca ggcggatgtg
    28981 gggttcgggc tggagggtct cgcggggagc atagatgtgc tcgtctcgaa cccgccttac
    29041 gtgaggagcg gagacatccg cgggctcgcg ccggaggttc gggactggga accgcatgcc
    29101 gcgctcgatg gcggcccgga cgggttgcgc ttctaccgcc ggatcttcac cgaagccccg
    29161 ttcctgctgg ttcccggcgc ggacgtggtg gtggaagtag gggacgatca ggcagaagcg
    29221 gtactcgggc tcgggcgcga ggcggggttt gtcccggtgg gggtaggccg ggatctcgcc
    29281 gggtcgcccc ggattgccct cctacgctgg gatggcgtgt gaagtacgtg gccgtgcgcg
    29341 aggcggcgga cgtgctgcgg agaggcggag tggcgctggt gcctaccgag acggttgtgg
    29401 ggctggtggc ggcggattcc ggcttcagca ggctgtggga gatcaaaggc cgggacgcgg
    29461 gcaaacctat cgcgctgctc tgcgaatcag cggatcaggc gttctcgctc gcacgcgaaa
    29521 cgccgccgct agccagagca ctcgccgccg cgtactggcc cggtccgctg acgctcgtac
    29581 ttgatgcccc cgagggaggt actgtcgggg tccgggtgcc ggacggaagt gctgtgcgcg
    29641 agttgctcgc cgtctatggc ggaccgctct acgccaccag cgccaacata tccggcggcc
    29701 ccgatgtgcg gaacatcgag caggttgatc cggtcgtggc ttcggcggtg gatgtggcgg
    29761 tgagaggcga gccgggcacg ggagaggctt cggcggtcgt ggacctttcg agcggccagg
    29821 tgaggctcct gcggccgacg gaggatctct cggaggcgag gttgcgccgg tttgcggaac
    29881 tggagtagat gccggacaca acggtttgag ggcgtccggc gcagcgttta tactctgcaa
    29941 agttgccagg ccggtaggga ggatgcgggg acatagaagc ccctcgatat cgctcacagc
    30001 caccagcatt ccgtcggcgg aacgccgatc gctttcccaa ccccgagcgg aggtagttag
    30061 atgataggcc cgtacaccga ggttgtagat tccgaggtcc aggatgcgct cgacggcgag
    30121 cttcagcgcc agcgcaccac catcgagctt atagcgagcg agaacttcgc ttccccggcg
    30181 gtcctcgcgg cgcagggctc ggtcctgacg aacaagtacg ccgagggtta tccgggaaag
    30241 cgttactacg gcggctgcga gcaggtggat aaggtcgagg tactcgccat cgagcgggcg
    30301 aagcaactgt tcggcgcgga tcacatcaac gttcagcccc actccggcag ccaggcgaac
    30361 gaggcggcct acgcggcgct catagatcct ggcgacgagg ttctctcgat ggacctggcg
    30421 cacggcggac acctgacgca cggaatgaag atcaacttca gcggccgcac ctatgacttc
    30481 gcccactacg gcgtcggcga agacggctac atcgactacg atcaggtacg ggacatcgcc
    30541 ctgcgccaca agccgcgcct catcctggcc ggggccagcg cctacccgcg tgtcatagac
    30601 ttcgagaagt tccgcgagat ctccgacgaa gtcggggcct ttttcctgac ggacatggcc
    30661 cacatcgccg ggctggtcgc cgccgatgtc cacccctcgc cgataccgca ctgcgagata
    30721 gtgaccacca ccacccacaa gacgctgcgg ggcgctcgcg gcggcatgat cctctgccgc
    30781 gaggagttcg cgaagaaggt caacagccgc gtcttccccg gcatgcaggg cggtccgctc
    30841 atgcacgcca tagccgggaa ggccgtcgcg ttcggcgagg ccctccagcc ggaattcaag
    30901 gactacgccc gcaggatcgt ggataactgc cgggcgatgg ctgaaggcct cctcgaaggc
    30961 ggcataaatc tcgtctccgg cggcacggac aaccacctga tcctggtgga cctgcgaggg
    31021 agcggcacga acggcaagga gctcgaagag aagctggagt ccatcggcgt tacctgcaac
    31081 aagaacatgg tcccgaacga cccggagccg cccacggtca ccagcggcgt ccggctcggg
    31141 actgcggcga tgacgacccg tggcatgggc gaggacgaga tgcgcgagat ctccgacata
    31201 atagtccggg ctgcgcgcgg agaggtcgag ggtctgcgcg accgggtggc ctcgctcacc
    31261 gacgctttcc cgctctatga gtgagacgaa gcccctggca gagaacttcc acctggtcga
    31321 tggcccactg agccgccaca agcttgccgt cctgcgcgac gagaagacgc gcacgccgga
    31381 cttccggcag gcgatgaagg aactggcgct gatcatggtc gccgaggcca cccgcgatat
    31441 gccgacccgc acggcgagta tccgcacccc tctgcaagag acggaggtcg aggagatctc
    31501 cggcccggtc tgcctcgtcc cggtcctgcg agccggcctc gggatgctcg acggggccct
    31561 cgccctgctc ccgacggcca cggtcggttt catgggactc ttccgtgacg aggagaccgc
    31621 cctgccggtc gagtactacg tgaacttgcc aaccaacctg gaggattatc tcgtcctggt
    31681 cctcgacccg atgctcgcca cgggcgggag cctttcggcg acgctggaca agctcaagga
    31741 gtacggggcg acctggatct cctgcctcca cgccgtctcc gccgtgccgg gcgtcgagcg
    31801 cgtcacccgc gagcaccccg acgttcagtt ctacaccgcc gctctcgacc cggaactgaa
    31861 cgacgccgcg ttcatcgtgc cgggcctcgg cgacgcgggg gacaggctct atggaaccct
    31921 ttaagaaccc ttcggtggat accaccaacg agcacgatac tctcaaaaaa gtccggccgt
    31981 cctgggacga gtacttcatg cggatcgcgc acgaagtcgc tacccgctcc acctgcccgc
    32041 gcctcgccgt cggcgccgtc atcgtacgcg acaagcgcat cctgacgacc ggctacaacg
    32101 gttctccgtc cgggttgccg cattgcgatg acgtggggtg cctcatccgc atcgtggacg
    32161 gccgcgagag ttgccagcgg acgctgcacg cggagcagaa cgccatcatc caggccgcct
    32221 atcacggggt ctccgtgacc ggcgcgtcca tttacagcac acaccagccc tgcctgatgt
    32281 gcgtgaagat gatcatgaac gccggcatac aagaagttca ctacgccggc ggctatccgg
    32341 acccgctcgc catggatctc gccactgagg gcggcttgac gatggtcaag tttgcgcccg
    32401 acgaaaactg agcgtttacg cttccggccc gtagagggtc agttcccaca ccctctccaa
    32461 cgtcccgacg acgatagcga tgcgctcctc tgagggcttt gcttgcggac ggccgaaggc
    32521 gtcgaggagg tagcgttcgg tcatcagcac cagcgcccgc gccgtctcct cggggtctat
    32581 ttctcctgat gagatgcccg cttccacgtc gcgcgatacc cggtccgcga cggcctgtat
    32641 gaggttctct atcagcccgt acctgtagac ttgctccacc cgcgagtcgt ggctcgccgc
    32701 gtcggcaatc gcgcgtagca cgggcccgta ctcgacgaac gtctccgcgc cccgccgcag
    32761 tgcttctccc aggctctcac tcgctggagc gccagcctct tcgccgccct ccagccagta
    32821 ccggtccacc gcgaagagca acgcgcctat accctccagt agccgcgtaa ccagatcgta
    32881 gcggtcccgg aagtaggcgt agaaggccgt cctggatagc cccgtgcggg tcattaccgc
    32941 ctccgccgtc aactcccgga atggatgctc tgccagaaaa gacctcgccg ccgccagtat
    33001 cgcccgctcg gcctgctgcg aagtatgccg ccgccgaggc ctccccgaca tgctcacact
    33061 gcgacctctt ccaacatata gccaatacta atggatcttc cacgttattg acacagcgtc
    33121 tattgacatc acgtcaatag ctgagtatca tgttattaca aaatcgtgcg aagggagagc
    33181 ggcatggcgg tcgcccgagt atccggtgga atgagggaag ttcggctgga gcatggtgtg
    33241 atccgttacc gtgaggcggg gagtgggccg gcgctggtct tcgttcacgg catactggtg
    33301 aatggaacgc tctggaggca tgtagtggcg cgtctctccg agaagttccg ttgcatcgtc
    33361 ccggacttgc cgctgggcag taattcgatc cccctggggg cggatgcgga tctcagcccg
    33421 gaggatgttg cccggatcgt cgccgatctc atcgagacgt tgaatctcga agatgtcacc
    33481 ctgattggca atgacacggg cggggccatc tgccagatag tgatagccga acacagtgat
    33541 aaagtctcgc gcctcgtcct caccaactgc gacgcttacg aagccttctt cccgctgctg
    33601 ctcagaccct tccaatacgc ggcccgaatc ttcggggtac g
//
LOCUS       DASSMB010000044         7514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1532069, whole genome shotgun sequence.
ACCESSION   DASSMB010000044 DASSMB010000000
VERSION     DASSMB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532069"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VFJ72_03155"
     CDS             complement(<1..808)
                     /locus_tag="VFJ72_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7478498.1"
                     /translation="MGEVNPHNRDLRPGALYKLLAYIVRKTLFSILVLFLASIVFFVL
                     VSLSGDPLLNLKQNPRIQQSDIVRLTAEYGLDQPLPVQYGIWMGDMLRGDLGTSFEQS
                     TSVNSIIGRRIWPTVLLMGSSLLFTVIVAIPFGIYSAIKKYSALDNAGTFLSFVGYSM
                     PTFWLGLILQLVLGVYLTSWAGVRIFYISGMTGTGGVVDLLQHLTLPLLTLSVISVAE
                     FSRFQRSAMLDVLSSDYLRTARAKGLSRRVVYLKHALRNALLPTVTVVALS"
     gene            complement(884..2539)
                     /locus_tag="VFJ72_03160"
     CDS             complement(884..2539)
                     /locus_tag="VFJ72_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7478499.1"
                     /translation="MSEKEARSGMERPPGAVGLTRRDFLKISGAGLAGAALLGVAGCG
                     GGGGGGQQGGGGGGGGDTLTVGYDQEPAILNQFIVGGDLAATNDMVAGIQQSPLMIMP
                     DLSLKPQLADGMPKVVNKDPLTIEYKLKDGITFSDGKPLTSEDARFTYDTIMDEKNKI
                     ITRLGWEDIEKFETPDKLTVRFTFKKDKPFAPWMTLLGGSETQILPKHIYEGKDFNKV
                     ANNEIVGSGPFKLKEWKKGQNLTWVRNDNYWGEKPALKEITFRFIPDTNTLNTSLQSG
                     EVDFISPPVDSGLIEKLKGYDGVTVQTKAGTVWEHIAFNLDKVDNLKMRQAIAYGINR
                     KQVVEQLLKGQVKPLESVLVPDQKPYYTPAWAEYTFDPDKAKQLAQQAKSEGAKMEIT
                     FSTTSDNKLRETLQQIVQQQLKDVGITVNIKNTAAETFFGKWTPEGTFEMGEWAWLAN
                     PDPTITSLFSADQQPPDGQNYYRYKNEQATKLMKESDKEVDVSKRADLLRQVQDLMVK
                     DLPLIPMYQRPVIQAYKENLSGPEVNPTLAGPFWNIGDWKFSG"
     gene            complement(2721..3635)
                     /locus_tag="VFJ72_03165"
     CDS             complement(2721..3635)
                     /locus_tag="VFJ72_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478500.1"
                     /translation="MSDVVVRPDERMDEVSTFMGDVRVDGPVAGDVGSAFGNVEIRHP
                     IGGTASSGWGDVYVYAPVRGVAADFGDVYIDAPVAGNVDVEHGNVRLGPDALVLGNLN
                     CGSGQVMGNRSAVRGAVLTGMASDFDRTSGDFKLLGFVGWLFAALVFVACSLLVAVLA
                     PGPVTSASRALEDSPVWSLILGVGTIPAAAVLGVLLAISVVGIPLLLLLAPAYLALIA
                     FGALVVAFFIGRKVVLATGRYRAGNALAAVVGALIVAAVYQIPFLGNLLLYALALLGT
                     GAAILALFSRRGPRIHTYQSPVRQRRDL"
     gene            complement(3767..4273)
                     /locus_tag="VFJ72_03170"
     CDS             complement(3767..4273)
                     /locus_tag="VFJ72_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET7478501.1"
                     /translation="MRGPCDCDPEIVFELADGTLSPARKSEVQEHLDACPECQELYQR
                     EVDLNAYLERLEISEARSRSVCQGVAMALPTRPVKARLLWGTLALALLLAALVALDLN
                     GTNPVTAATSAVDVFWSIVSGFAHLAVTVFSMAGPVILIALAFGAVADLVIAAAVLSA
                     ARRSSRQA"
     gene            complement(4270..4872)
                     /locus_tag="VFJ72_03175"
     CDS             complement(4270..4872)
                     /locus_tag="VFJ72_03175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7478502.1"
                     /translation="MTLGGWRERVRGLSVRREALDDNDLVVRTLAGDVRSYEELVRRY
                     ERLVGRILYPYARREVSVEDLVQETFLRAYDRLETFNPDYRFKTWLLAIANNLGVDTL
                     RRRREVVEFNPETHAGSASSAGGPESLALEADRSRVVQEAIMTLPETYSVPLILRYTE
                     EMSYAEIADVLDLTVQAVKSRLFRARNMLAEKLREDGEGL"
     gene            4945..6174
                     /locus_tag="VFJ72_03180"
     CDS             4945..6174
                     /locus_tag="VFJ72_03180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET7478503.1"
                     /translation="MAEKNGSSLTAIKPAPVLAALIAAHTLVYLAVTYLAVSNGPAFQ
                     VEIFREYADKISAGNLPYADFLYEYPPLSLFILILPRLLVSDPAAYAALFGAEMLLFD
                     IIILLALSRIGVKPLLLYGVGLLLFWRLPYIRHDLVPVACATTGALLLLRGRTLWAAA
                     LWGVGGAIKLYPIVAVPALAAGAALRQTAARWAVAGAVFAGGILWGIPAFGPRALEFL
                     TYHSDRPAMIESLPANLLLLLPGGRVTHSFGSFNVVGPLGDSLVGFFEVFQLVVTVLA
                     LLVVWRQGHGAEPRTVAVRGAAVATFAFAVFGKVLSPHFLFWPLPLVAISTALGGLRF
                     PRATWALYFTVILVTTAINEQYWAIDENLPYFTAMLTARNLLLLPLFGLLLLRPPEDQ
                     EEQKHRAHTGGNDQRIR"
     gene            complement(6073..>7514)
                     /locus_tag="VFJ72_03185"
     CDS             complement(6073..>7514)
                     /locus_tag="VFJ72_03185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7478504.1"
                     /translation="FGTENLDRIGDAYRMASYALFLAAIFASFRFMRREFGVAAAWGA
                     ALSMPLIPALFGFARLGQLDGAVSSMYLISALALHRMFTYGTRRGVVWAGVLLGLAFA
                     TKLNVFTLVPATLLWAVIYHRERAAFARLFAAFGMGAAVFFGIWPWLWRDPVGRTLEF
                     LTWTAGLQNERFTYYLGQWWAGAPWHYPLVMLIAIVPLAVAAAGVCGVARFFCEARNP
                     AAGWVILNLALVLAVAGSGLVPIYGGPRQFLAAFPLWALCAGAGIGWLAARVRRPAVA
                     LAAYAALSLPGILWTGVANSLEYYGEAVGLIPGANSLGFETTYLADTYKPAVEWLNED
                     APRGATVYAQAGTFAALETYRRVGDMREDLRPAYLTPIAPAEYRHDEKPRKDSYFLFL
                     PRQSIYTDQMLALDREKPLYQYVKGGVPLIKVYSGQTVAKTLTIEGKPAARDVGLPNA
                     LVVASGVSAVFLFLLIFRRAQQEKPEERE"
BASE COUNT         1352 a         2466 c         2346 g         1350 t
ORIGIN      
        1 tgctgagagc cacgacggtc acagtcggca gaagcgcgtt cctgagcgcg tgcttgagat
       61 agaccacccg acggctcaga cccttggccc gcgcagtccg caggtagtcc gaggagagca
      121 catcgagcat ggcgctgcgc tggaacctgc tgaactccgc gacgctgatc accgagagcg
      181 tcaggagcgg aagggttagg tgctgtagca gatccaccac ccctcccgtg ccggtcattc
      241 cggagatgta gaagatgcga acgccggccc aactcgtcag atagacgccc aggactagct
      301 gcaagatcaa accgagccag aaggtcggca tgctgtaccc gacgaaggat aagaacgtgc
      361 ccgcgttatc cagagcgctg tacttcttta tcgccgagta gatgccaaac ggtatggcga
      421 cgatcacggt gaataacagc gaggagccca tcaggaggac cgtaggccat atcctgcgac
      481 cgatgatcga gttcaccgag gtgctctgct cgaagctggt ccccaggtct ccccttagca
      541 tgtcgcccat ccagatcccg tactgcaccg ggagcggctg gtccagcccg tactcggcgg
      601 ttagccgcac tatgtccgac tgctggatac gcgggttctg cttgaggttg agcagcgggt
      661 ccccagagag acttaccagc acgaagaata caatgctggc gagaaaaagc actaagatac
      721 tgaacagtgt ttttctgact atatatgcaa gcaacttata gagtgccccc ggtcgaaggt
      781 cccggttatg gggattgacc tcccccatcg atccaggatt atatattaac ggcagtatgc
      841 ccggcccgga gagtctccgg gccgggcgcc tcgtgttagc ctcctagccg gagaacttcc
      901 agtcgccgat gttccagaac ggcccggcga gggtcgggtt gacctccggc ccggagaggt
      961 tctccttgta cgcctggatg accggccgct ggtacatcgg gatgagcggc aggtctttca
     1021 ccataaggtc ctgcacctga cggagcaggt cggcccgctt gctcacgtcc acttccttat
     1081 cggactcttt catcagcttc gtggcctgct cgttcttgta ccggtagtag ttctggccgt
     1141 cgggcggctg ctgatcggca ctgaagagcg aggttatcgt gggatcggga ttggcgagcc
     1201 aggcccactc gcccatctcg aaggtcccct ccggggtcca cttgccgaag aaggtctccg
     1261 cggcggtgtt ctttatgttc accgtgatgc cgacgtcctt gagctgctgc tggactatct
     1321 gctgcagcgt ctcgcgcagc ttgttgtcgg aggtggtgga gaaggtaatc tccatcttgg
     1381 ccccctcgct ctttgcctgc tgcgcgagtt gcttggcctt gtccggatcg aaggtgtact
     1441 ccgcccaggc gggcgtgtag tacggtttct ggtccgggac caggacgctc tccagcggtt
     1501 tgacctgtcc cttgagtagc tgttccacga cctgcttgcg gttgatgccg taggcgatgg
     1561 cctggcgcat cttgaggttg tccaccttgt ccaggttgaa cgctatgtgc tcccagaccg
     1621 ttccggcctt ggtctgcacc gtaacgccgt cgtagccctt gagcttctcg atgaggccgc
     1681 tgtccaccgg cgggctgata aagtccacct ctcccgactg caacgaagtg ttcagcgtgt
     1741 tggtgtcggg gatgaagcgg aaggtgatct ccttgagcgc gggcttctcg ccccagtagt
     1801 tatcgttacg cacccaagtc aggttctggc ccttcttcca ctccttgagc ttgaacgggc
     1861 cgctgccgac gatctcgttg ttggcgacct tgttgaagtc cttgccctcg tagatgtgct
     1921 tcggcaatat ctgagtctcg gagccgccca gcagtgtcat ccagggcgcg aaaggcttgt
     1981 ccttcttgaa ggtgaaacgg accgtcagct tgtccggcgt ctcgaacttc tctatatcct
     2041 cccaaccgag acgggtgatg atcttgttct tctcgtccat gatggtgtca taagtgaacc
     2101 gggcgtcctc gctcgtgagc ggcttgccgt cggagaaggt gatcccgtcc ttcagcttgt
     2161 actcgatggt gagcgggtcc ttgttcacga ccttcggcat cccgtcagcg agttgcggct
     2221 ttagggagag gtcgggcatg atcatgagcg gcgactgctg gatcccggct accatgtcgt
     2281 tggtagccgc caggtcgcca ccgacgatga actggttgag gatcgccggc tcctggtcgt
     2341 agccgaccgt cagagtgtct ccgccacccc cacctccgcc accttgctgg ccgccgcctc
     2401 cgccaccacc gcagcccgcg acacccagta gagcggcgcc cgctagaccg gctccgctga
     2461 tcttcaggaa atcccgccgg gtcaacccca cagcgcccgg cggtcgctcc atcccggacc
     2521 tggcttcttt ctcgctcatg gattctcctc tctcatctat gtgtgctcgc tatgcgctcc
     2581 cccggatgca atccctgcgc aacgatatac gaatgcgcct taccgcctgg tattttaggc
     2641 tgacttatgg cgtcttgccg cagtataagc ctatgtttaa tgatgatgca acactgcaca
     2701 atagtatcag tcgctgcatg ctaaagatca cggcgctgcc gtacgggcga ctggtaggtg
     2761 tggatgcgcg gtccacggcg ggagaagaga gccagaatgg ccgcgccggt accgagaagg
     2821 gcaagagcgt agagcaggag gttgccgagg aacggtatct ggtagacggc ggccacgatc
     2881 agggccccaa ccacggcggc cagggcgttg cccgcccggt agcggcccgt ggcgaggacc
     2941 accttgcgcc cgataaagaa cgcaaccacg agcgccccga acgcgatcaa cgccaggtaa
     3001 gccggagcca ggaggagcag caagggtatt cccaccaccg agatcgcgag cagcaccccc
     3061 agaacggccg ccgccggtat ggtcccgacg cccaggatca gggaccacac gggagagtcc
     3121 tccagcgcgc gcgaggccga cgttacgggc cccggagcca gcaccgcgac cagcaggctg
     3181 caagccacga agacgagcgc cgcgaacagc catcccacga aaccgagcag cttgaagtca
     3241 cccgaagtac ggtcgaagtc cgaggccata cccgtcagca cggcgccccg cacggcgctc
     3301 cggttcccca tgacctgccc gctcccgcag ttgaggttgc ccaggaccag agcgtcaggg
     3361 cccagccgca cgttaccatg ctccacgtcc acgttcccgg ccaccggggc gtctatgtat
     3421 acgtctccga agtccgccgc cacgcccctt accggcgcgt agacgtagac atcgccccac
     3481 ccggaactgg cagtgccgcc aatagggtgc cggatctcga cgttgccgaa ggcggacccg
     3541 acatctccgg ccaccgggcc gtccaccctc acatcgccca tgaatgtcga gacttcatcc
     3601 atccgctcgt ccggccgcac gaccacgtcg ctcatggccc ggttctcgaa ggcctgcgcg
     3661 ggcagcggaa gcaggaccaa catccccaag agaagagccg agagtgataa gagtagtccc
     3721 gttgagaatc tcatcgagaa tcggggccgc aaggttgcgg ggtggatcat gcctgccggg
     3781 agctgcggcg cgccgccgaa agaacggccg ccgcgataac caggtccgca accgccccga
     3841 acgccagcgc gatcagaata acgggtcccg ccattgagaa gaccgtgacc gccagatggg
     3901 cgaaccccga gacgatactc cagaaaacgt ccaccgcgct cgtcgcggcg gtgacggggt
     3961 tggtgccgtt gaggtctagg gcgaccagcg cggcgagtaa cagggccagg gcgagcgtcc
     4021 cccacaggag acgggccttt acgggccggg tcggcagcgc catcgcgact ccctggcaca
     4081 ccgagcgcga acgggcctcg gagatctcca gacgttccag gtaggcgttc aaatctacct
     4141 cgcgctggta cagctcctgg cactcggggc aggcgtccag gtgctcctgg acctcgctct
     4201 ttcgagcggg agagagcgtc ccgtcggcga gctcgaagac aatctccggg tcgcagtcgc
     4261 atggtcctct cacaggccct caccgtcttc tctcagcttc tcggcgagca tgttgcgggc
     4321 gcggaagagc cggctcttca cggcctgaac cgtcaggtcc agcacgtcgg ctatctcggc
     4381 gtaggacatc tcctcggtat agcgaaggat caagggcacg ctgtaggtct ccgggagcgt
     4441 catgatcgcc tcctgcacga ccctggaacg gtcggcctca agagccaggc tctccgggcc
     4501 tccggcgctg ctggcggagc cggcgtgcgt ctccgggttg aactcgacta cctcccggcg
     4561 gcggcgcagg gtgtccaccc ctaagttgtt ggcgatggcg agcagccagg tcttgaagcg
     4621 gtagtcgggg ttgaatgtct ccaggcggtc gtaggccctg aggaaagtct cctgcaccag
     4681 gtcctccacg gagacctcgc ggcgcgcgta ggggtagagg atgcgcccga cgagccgctc
     4741 gtagcggcgg acgagctcct cgtacgagcg gacgtcaccg gccagggtgc ggacgacgag
     4801 gtcgttgtcg tccagcgcct cgcgccggac cgagaggccg cggactcgct cgcgccagcc
     4861 tcccagggtc actgtggatg cgtgctccat acgagaatac taatacgaac ctcaccggta
     4921 aaagtttctg agagaatgta cggtgtggcc gaaaaaaatg gatcatccct cacagcgatc
     4981 aagcctgccc cggtccttgc cgccctgatc gccgctcata ccctcgtcta cctcgccgtc
     5041 acatacctcg cggtatctaa cggaccggcc tttcaggtag agatattccg ggaatatgcg
     5101 gacaagatct ccgctggcaa ccttccctac gccgacttcc tctacgagta tccacccctt
     5161 agcctgttca tcctcatctt accgcgcctc ctggtctcgg acccggcggc ctacgccgcg
     5221 ctcttcggcg cagagatgct cctcttcgac ataataatcc tcctggcgct ctcccggata
     5281 ggcgtgaaac ccctcctcct ctacggcgtg ggccttctcc tgttctggcg cctcccctat
     5341 atccgccacg acctcgtccc ggtcgcctgc gccaccacgg gcgcgcttct cctgctgcga
     5401 ggccggacgc tgtgggccgc ggcgctctgg ggcgtgggcg gggcgatcaa gctatacccc
     5461 atcgtcgccg tcccggctct cgccgccgga gcggccttgc ggcagacggc ggcgcgctgg
     5521 gccgtggccg gcgccgtatt cgcgggcggc atcctctggg gaatcccggc cttcggaccg
     5581 cgggcgctgg agttcctaac ctaccactcc gaccgtccgg ccatgataga gagcctgccg
     5641 gcgaacctgc tcctcctgct gcccggcggg cgagtaaccc actctttcgg gagcttcaac
     5701 gtggtcggcc cgctggggga cagcctggtg gggttcttcg aggtcttcca gctcgtcgtg
     5761 acggtgctgg cgttgctcgt ggtgtggcgg cagggacacg gagcggagcc gcggacggtc
     5821 gccgtgcgcg gggcggcggt cgcgacgttc gccttcgcgg tcttcgggaa ggtgctctcg
     5881 ccgcacttcc tgttctggcc gctgccgctc gtagcgatct cgacggcgct cggcgggttg
     5941 cggttcccgc gcgctacctg ggcgctctac ttcaccgtca tcctcgtaac gaccgcgatc
     6001 aacgagcagt actgggccat agatgagaac ctcccctact tcaccgccat gcttacggcg
     6061 cgcaacctgc tgctactccc gctcttcggg cttctcctgc tgcgcccgcc ggaagatcag
     6121 gaggaacaaa aacaccgcgc tcacaccgga ggcaacgacc agcgcattcg gtaacccaac
     6181 gtcgcgggcg gcaggtttac cttctatcgt cagggtcttc gcaaccgtct gaccagagta
     6241 cacctttatc agcggcactc cccctttcac gtactggtag agcggcttct ccctgtcgag
     6301 cgcgagcatc tggtccgtgt atatggactg gcgcggcagg aacaggaagt aggagtcctt
     6361 gcgcggcttc tcatcgtgcc ggtattcggc cggcgctatc ggcgtgaggt aggcaggacg
     6421 cagatcctcc ctcatgtccc cgacccggcg gtaggtctca agcgccgcga aagtcccggc
     6481 ctgggcgtag accgtggctc cccgtggcgc gtcctcgttc aaccactcga cggccggctt
     6541 gtaggtgtcc gcgagatatg tggtctcgaa gcccagagag ttcgcgccgg ggatcaggcc
     6601 gacagcctca ccgtaatact ctaaagagtt cgccaccccc gtccagagga tgccggggag
     6661 cgagagcgcc gcgtaagccg caagggcgac ggcagggcgg cgcacgcgag cggcgagcca
     6721 gccgatgccg gctccggcgc acagggccca gagcgggaag gcggcgagga actggcgggg
     6781 gccgccgtag atcggcacga gccccgagcc cgcgaccgcg aggacgagcg ccaggttcag
     6841 gatgacccag cccgccgccg ggttcctcgc ctcgcagaag aacctcgcca caccgcacac
     6901 gcctgccgcc gcgacggcga gcgggactat ggcgattagc attaccagcg ggtagtgcca
     6961 cggcgctccg gcccaccact gccccaggta gtaggtaaag cgttcgttct ggagcccggc
     7021 cgtccaggtc aggaactcca gggtgcgtcc gaccgggtcg cgccagagcc acggccagat
     7081 gccgaagaag acggcggctc ccatcccgaa ggcggcgaac agacgagcaa aagcggcgcg
     7141 ctcgcggtgg tagatcactg cccacaacag cgtcgccggt acgagggtaa agacgttcag
     7201 tttcgtggcg aacgccagtc ccagcaagac tccggcccac acgacgccgc ggcgtgttcc
     7261 atacgtaaac atccggtgga gggcgagcgc ggagatcagg tacatcgaag agacggcacc
     7321 gtcgagctgc ccgagccggg cgaacccgaa gagcgccggt atgagcggca tggaaagagc
     7381 agctccccac gccgccgcaa ctccgaactc ccggcgcatg aagcggaagg acgcgaatat
     7441 ggcggcgagg aagagcgcgt aggaggccat ccggtaggcg tcgccgatgc ggtcgaggtt
     7501 ctccgtcccg aaga
//
LOCUS       DASSMB010000045         9415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1278149, whole genome shotgun sequence.
ACCESSION   DASSMB010000045 DASSMB010000000
VERSION     DASSMB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278149"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VFJ72_03190"
     CDS             <1..749
                     /locus_tag="VFJ72_03190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7478505.1"
                     /translation="EIYVASKVPPKNGVWPAPSGLSPENVFPGDYVRECTEQSLKNLG
                     LETIDVQQFHVWQDEWVGEGSWLEAVEELKREGKIRFFGVSINDHQPANAIKLIQSGV
                     VDTVQVIYNVFDQSPEDQLLPVCEEHGVGVIVRVPFDEGALTGAITPETTFAEDDFRN
                     GYFRGDRKRQVYERVQEIVRDLDISEGEIAEVALRYILSDPAVSTVIPGMRSMRNVER
                     NMAVADGRGLPDEQVQKLKAHRWDRNFYVQ"
     gene            818..1720
                     /locus_tag="VFJ72_03195"
     CDS             818..1720
                     /locus_tag="VFJ72_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7478506.1"
                     /translation="MRIFGKRRFGVLAFVLLVVFWSSAFSVVKIGLDYSPPLLFAGLR
                     TMIGGIVITVAAALWGGKPHFRRDWWIFLLLAAFNVALFIGGQTFAVLNLSSGTAAVL
                     IYLQPILVGFLAWMILGESLTPMKVVGLLLGFAGIVAVSSGGLTEGGITPVGVAFGVA
                     SALFWALGTVFFKKYEARVSTLWAVAVPFLIGGVALMAVGILIEPLSGITWNGAFEAS
                     LLYSALIGTGFAWLLFFGLVRAGEASRVASYIFVVPLAAVAIGAVFLGEGVGPSLLVG
                     AGLVVAGIYLVNRRPIAGGPVNPR"
     gene            complement(1825..2613)
                     /locus_tag="VFJ72_03200"
     CDS             complement(1825..2613)
                     /locus_tag="VFJ72_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478507.1"
                     /translation="MRKRERTLLLVGSGLLALALLAGACAAKGPAQGGSTGRSQETTV
                     VQAGAPATSYDPKIDPANFVRLVDNPLFPLKPGTVYELQDKSSRGVEHETVTVTDHKK
                     TILGVSTTVVEDVLKLHGQLEEKTSDWYAQDKQGNVWYFGEDTATYKNGKVDSREGSW
                     KAGVHDARPGIIMNANPQVNDAYRQEYYRGHAEDMFWVIDDHGSVQAPYGSFHNAVLT
                     LEWTPLEPNVVSEKYYAPGVGLVAERDLAGGNEHFELVRVTRSG"
     gene            complement(2775..4199)
                     /locus_tag="VFJ72_03205"
     CDS             complement(2775..4199)
                     /locus_tag="VFJ72_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7478508.1"
                     /translation="MSLPIRWRLTLFIALVIGAILIVLGLALYLLSRSTLLAEIEDTT
                     QGRAASVARAVESGEKLSDDDLQRLTVDGVCVIVRDGNGGVLRKINLPEKGRADDTVW
                     RRALSSGKSASGTARLLGDNPYYIHAVPVAPSNGPARVVEAAKPYEPTLEVLESFEVL
                     LGAGIAAALLLSIGGAYLLAVAALRPVDTVTSVAREMSESDLSKRLPVANSGDEIGRL
                     ATTINGLFSRLETAFADREETLERQRRFAADASHELRTPLTAISGHARMLDEWALEAD
                     PERAKRSVGAIRREAGRMRNLIESLLILTRGDEGDGLEVGRHDFGEVAEEAIHTARAA
                     AADKVTVEYFPPERKVEAVFDRDRIRQAALILVDNAIRYTPEGGSVRVRVGEENGQVA
                     LAVSDTGVGVPEEQLPLIFERFYRVEESRTGQGPSAGGAGLGLSIARQVAEAHGGTIE
                     VESKPDAGSTFTLLLPRAPHGREG"
     gene            complement(4201..4878)
                     /locus_tag="VFJ72_03210"
     CDS             complement(4201..4878)
                     /locus_tag="VFJ72_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7478509.1"
                     /translation="MRPGTRVLLVEDDRSIADFVEPELERLGFRVRCAYDGRSGLEEF
                     QDFRPELVILDIMLPKLDGVSVLKEIRGRGSRVPVIMLTARDTLPDKVHSLDNGADDY
                     LTKPFDVEELLARLRALLRRAEGEEILRVADLEVNTATREVRRGEREIALTPREFDLL
                     EFMARNPRRVLSRELLLSRVWDQEFGLTTNVVEVYIVYLRKKVDAPGEPKLIRTIRGA
                     GYALKGV"
     gene            5201..5635
                     /locus_tag="VFJ72_03215"
     CDS             5201..5635
                     /locus_tag="VFJ72_03215"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7478510.1"
                     /translation="MQQSGVDQEFRPPVPPFDEASAREKVKKAQDAWNTRDPEKVALA
                     YTPDSKWRNRDEFFEGREAIVEFLKRKWARELDYRLMKELWCYQGNRISVRFEYESRD
                     VDGQWWRSHGNEHWEFDERGLMRRRDASINDYKIEESDRRIF"
     gene            5694..5984
                     /locus_tag="VFJ72_03220"
     CDS             5694..5984
                     /locus_tag="VFJ72_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HET7478511.1"
                     /translation="MIDHLVLIAVRDDCSPEEVADLVDSARKLKDSVPGVVDLSIGED
                     FSGRGGEYTHGLFARFEDRDGLQGYLKHPDHIAVVEKLDRYTNGRLVLDYEA"
     gene            6023..6448
                     /locus_tag="VFJ72_03225"
     CDS             6023..6448
                     /locus_tag="VFJ72_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478512.1"
                     /translation="MRVNRTQGLVLGFFAFVWVSLVVILMTAPEIYDQALRMSAGGHR
                     AFEIAFLAAISAFIALLSVGTVRRWRWTFWLIFVAFIFGLLRVPASILQLIGLLRAQG
                     PVWYTLFQAALGLVQFAIALAMLAGYRRNGVWGSFGSPE"
     gene            6459..6779
                     /locus_tag="VFJ72_03230"
     CDS             6459..6779
                     /locus_tag="VFJ72_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7478513.1"
                     /translation="MEYLKITPGELKSRLDSGERPVLLDVREPWEYEAARIEGSRLIP
                     LGEMPHRVSELDPARETVVICHHGARSAYVAQALTRSGFSKVLNLEGGLDAYSRVDAS
                     VPRY"
     gene            complement(6831..7205)
                     /locus_tag="VFJ72_03235"
     CDS             complement(6831..7205)
                     /locus_tag="VFJ72_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478514.1"
                     /translation="MGDQIRELLADPAAALVKESFTGVSARWWWERRISGGIELCQEL
                     DPERMSREIAESTGRSPTEVMQVLREELGLEDTDPVVLTFEVPGDATAREAARALAEH
                     SADPEGLAAPLYRRVEEIVKAG"
     gene            7304..7960
                     /locus_tag="VFJ72_03240"
     CDS             7304..7960
                     /locus_tag="VFJ72_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HET7478515.1"
                     /translation="MLLVRDSMTREVVTLTPQTTAAEALALCRDRRIRHLPVLENGRL
                     AGMVSDRDLRSATPALGDPARAGALERIKVGDVMSREVSTSHPDDPIEQAANEMREKK
                     IGCLPVVEHGTLVGIVTSSDVMEALTYLVGAHEPGSRMEVSVPDRPGTLAGAAGIFGM
                     CGINIVSVVMGARTEPASKDALPERLVVFRVDTIDTSEVAQYLEDAGYSVLWPPRKSA
                     "
     gene            7917..9137
                     /locus_tag="VFJ72_03245"
     CDS             7917..9137
                     /locus_tag="VFJ72_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HET7478516.1"
                     /translation="MPATACYGRPGNPRDGDCLLSGKSAIVHDRRLESYGFGDDHPFN
                     PLRIRMTLELCESLGLFDGYEFVGSEPASDEDLRTVHSLTYIRSVQQAGRGLADLADL
                     ISYGIGTPDNPIVPDIHEAGADVCGAVLKAARMVISGEVDHALCISGGLHHALRSQAS
                     GFCFYNDAGVAIARLKQEHPGIRVAYLDTDAHHGDGVQWMFYDDPEVLTISMHESGRY
                     LFPGTGGVEERGRNGGVGYSVNVPLEPFTDDSSWISCFEAVVPEALRTFGPDLILSQN
                     GCDGHKLDPLTHLSATTRLYEHVPRRVHELAHELCEGRWVATGGGGYDIWRVVPRAWT
                     ILWATLSHQRIPEKVSGEWLDKWGARSPVRLPTLMRDDPADYPEISRAEEIAERNSRT
                     VAHVLESVLARIKA"
     gene            9222..>9415
                     /locus_tag="VFJ72_03250"
     CDS             9222..>9415
                     /locus_tag="VFJ72_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HET7478517.1"
                     /translation="MPAMLEILGASGTETAPKETTRRKMTTEPPYKVILHNDDYTPME
                     HVIQVLRKVIPRMSLRRAVS"
BASE COUNT         1600 a         2929 c         3129 g         1757 t
ORIGIN      
        1 aggagatcta cgtcgccagc aaggtcccgc cgaagaacgg cgtctggccc gcaccctccg
       61 gcctgagccc ggagaacgtc ttccccggcg actacgtgcg cgagtgtacc gagcagagcc
      121 tcaagaacct ggggctggag acgatagacg tgcagcagtt ccacgtctgg caggatgagt
      181 gggtcggcga gggtagctgg ctggaggcgg tcgaggagtt gaagcgggag ggtaagatcc
      241 gcttcttcgg cgtctccatc aacgaccacc agcccgcgaa tgccatcaag ctcatccaga
      301 gcggcgtggt ggatacggtg caggtcatct acaacgtctt cgaccagagc ccggaggacc
      361 aactattgcc ggtgtgcgag gagcatggag tgggggtcat cgtgcgggtc ccgttcgacg
      421 agggcgcgct cacgggggcc atcacaccgg agacgacgtt cgccgaggac gacttccgca
      481 acggctactt ccggggcgac aggaagcggc aggtctacga gcgggtgcag gagatcgtac
      541 gtgacctgga catctcggag ggcgagatcg cggaggtcgc gctacgctat atcctgagcg
      601 acccggcggt ctcgaccgtg ataccgggga tgcgctcgat gcggaacgtg gagcgcaaca
      661 tggccgtcgc agacggacgg gggctaccgg atgagcaggt ccagaagctc aaggcacacc
      721 gctgggaccg caacttttac gtgcaatagg agatcttttc gggactccgg ctaggcgccg
      781 gggtctcgga tctcaggaac tatttgaaga ggagattttg cgtatatttg gtaagcgccg
      841 ctttggcgtt ctggcttttg ttttgctggt ggtcttctgg agctcggcgt tctcggtggt
      901 caagatcggg ctagactact cgccgccgct gttgttcgct gggttgagaa cgatgatcgg
      961 ggggatagtc attacggtcg ccgccgctct ctggggcggt aaacctcact tccgacgcga
     1021 ctggtggata ttcctgctcc tggcggcgtt caatgtggcg ctgttcatcg gggggcagac
     1081 cttcgcggtg ctgaacctct cctcggggac ggcggcggta ctgatctatt tgcagcccat
     1141 cctggtcggg ttcctggcgt ggatgatcct gggggagtcg ctgacgccga tgaaggtcgt
     1201 ggggctcctg ctcgggttcg cgggtatcgt ggctgtcagc tccggcgggt tgaccgaggg
     1261 tgggatcaca cccgtcggcg tggctttcgg ggtagcatcg gcgctcttct gggcgctggg
     1321 gacggtgttc ttcaagaagt acgaggcgcg ggtctcgacg ctctgggccg tggccgtgcc
     1381 gttcctcatt ggcggcgtcg cgctcatggc cgtcgggata ctgatcgaac cactctccgg
     1441 catcacctgg aacggggcct tcgaggctag cctgctgtac tcggcgctca tcgggaccgg
     1501 attcgcgtgg ctgctcttct tcggactcgt gcgcgcgggg gaggcgagcc gggtggcgtc
     1561 gtacatattc gtcgtcccgt tggcggccgt cgcgatcggc gcggtcttcc tgggtgaggg
     1621 cgtggggcca tcgctgctcg tgggcgccgg actcgtcgta gccgggatct acctggtcaa
     1681 ccgccgccct atcgcgggcg gccctgtgaa ccctcgctga accgcatctg ctgatggcgt
     1741 ctgctgatgg catctgctga tcgaacctcg ccgcaggtaa gggagagcga acagtgtagg
     1801 agtcggggga gagcctggca gtttttagcc gctgcgtgtc acgcgcacaa gctcgaagtg
     1861 ctcgttgccg ccggcgaggt ctctctccgc cacgaggccc acgcccggag cgtaatactt
     1921 ctcggatacg acgttgggct caagcggcgt ccactccagg gtaaggacgg cgttatggaa
     1981 agagccgtag ggagcctgga ccgacccatg atcgtcgatc acccagaaca tatcctcggc
     2041 atgacctctg taatactcct gcctgtaggc gtcgttgacc tgggggttgg cgttcatgat
     2101 gatgccgggc ctggcgtcgt ggacgccggc tttccaggag ccttccctgc tgtcaacctt
     2161 gccgttcttg tacgtcgcgg tgtcctcgcc gaagtaccac acgttgccct gcttatcctg
     2221 agcgtaccag tcggacgtct tctcttcgag ttgaccgtgc agcttgagga catcctccac
     2281 gacggtcgtc gagaccccca ggatagtctt cttgtggtcg gtgaccgtta cggtttcgtg
     2341 ctcgacgccg cggctggact tgtcttgtag ctcgtagact gtaccgggct tgagcgggaa
     2401 caagggatta tccacgaggc gcacaaagtt cgcgggatct atcttcggat cgtagctggt
     2461 agccggggca cctgcctgca ccacggttgt ctcttgcgag cgtccggtgg atccgccctg
     2521 cgccggccct ttggcggcgc acgctccggc gagcagcgcg agcgcgagca atccgcttcc
     2581 aaccaggagc agggttcttt ctctctttct catcggtaga tctctcctcg ttcgcctaca
     2641 gatgaggcca ttatccaggc ggttctttag aagaagctga ggaagacctt agagtcgtat
     2701 tagaaaaacg tctccgcgag atcacgccgg atggagcgga tacgaaaccg cggatcggcg
     2761 ctgtaaagag cggttcagcc ttcccggccg tggggcgccc gtggcaggag cagggtgaaa
     2821 gtcgagcctg cgtccggctt gctctcgacc tcgatcgtgc cgccgtgggc ctccgcgacc
     2881 tggcgggcta tggagagccc gagccccgcg ccgcccgccg aagggccttg ccccgtacgg
     2941 gattcctcga cccggtagaa gcgctcgaag atgagtggca gttgctcctc ggggacgccg
     3001 acgccggtgt cggagaccgc gagcgcgacc tgaccgttct cctcgcccac cctcacccgg
     3061 acgcttccgc cttccggcgt gtacctgatc gcattgtcca ccaggatcaa ggcggcctgc
     3121 ctgatgcggt ccctgtcgaa cacggcttct acttttcgtt caggagggaa atactcgacg
     3181 gtcactttat ctgccgccgc ggccctcgcc gtatgtatgg cttcttcggc gacctcgccg
     3241 aaatcgtggc gtccgacctc cagaccgtcg ccttcatcac cgcgggtgag tatcaggagg
     3301 gactcgatca ggtttcgcat tctcccggcc tcgcggcgga tggcgccgac gctccgcttc
     3361 gccctctccg ggtccgcctc gagcgcccac tcgtcgagca tcctggcgtg gccgctaatg
     3421 gcggtcaggg gcgtgcgcaa ttcgtggctg gcgtcggcgg cgaagcgccg ctggcgctcc
     3481 agagtctcct ccctgtcggc gaaagcggtt tcgagacggg aaaagagacc gtttatcgtc
     3541 gtggcgaggc gcccgatctc gtcgccggag tttgctacgg gtagccgttt cgagaggtcg
     3601 ctctcgctca tctcccgggc aacactcgtc acggtgtcga cgggtctgag ggccgctacc
     3661 gcgaggaggt aggcgccgcc gatggacagg agcaaggcag cggcaatgcc ggcgcccagg
     3721 aggacctcga aagattccag gacctccagg gtaggctcat agggcttggc agcctcgacg
     3781 acccgcgcgg gtccgttgga cggggccacc ggtacggcgt gtatgtagta ggggttgtct
     3841 cccaggagcc gggccgtacc cgaggcggat ttcccggaac tgagcgctcg ccgccatacg
     3901 gtatcgtcgg cacggccctt ctctggcagg ttgatcttcc gaagaactcc gccgtttccg
     3961 tcccggacga tcacgcacac cccgtcgacg gtcagacgtt ggaggtcgtc gtcgctcaac
     4021 ttctctccgg actcgacggc tcgggctacc gaggccgcgc ggccctgggt ggtatcctcg
     4081 atctcggcga gcagggtgct cctgctcaga aggtagagcg cgagacccag gactatcagt
     4141 atcgcaccga tcacgagggc gataaacagc gtcaaccgcc accggatcgg taaggacatc
     4201 ttatactccc ttcaaggcgt atcccgcgcc gcggatggtg cggattagct tcggttcccc
     4261 cggcgcatct actttcttcc tgaggtagac gatatagacc tcgacgacgt tggtggtgag
     4321 accgaactcc tggtcccaca ccctgctcaa gagcagttct cgcgagagaa cccgccgggg
     4381 gttgcgggcc atgaactcca gcaggtcgaa ctcgcggggg gtgagagcta tctcccgctc
     4441 cccgcgtcgg acctcacggg tggcggtgtt tacctccagg tcggctaccc tcagtatctc
     4501 ctcgccttcc gcgcgcctca gcagcgcccg cagccgggcg agtagctctt ccacgtcgaa
     4561 cggcttggtc aggtaatcgt cggcgccgtt atctaggctg tggaccttgt ccggcaaggt
     4621 atctcgggcg gttagcatga tcaccggcac ccggctgccc cggccacgga tctcttttaa
     4681 tacacttaca ccgtccagct tcggcagcat gatgtccagg attaccagtt cgggcctgaa
     4741 gtcctggaac tcttcgaggc ccgagcggcc gtcgtaagcg caccgcaccc ggaagccgag
     4801 acgttcgagc tcgggctcga cgaagtcggc gatcgagcgg tcgtcctcta cgagcagcac
     4861 gcgcgttccc ggtctcatac tcgcctcgtc caccctttcc ccggtattct aatccctgcg
     4921 ctcctccgaa ggttgtttct caggacggtc gttcgggtcc cggtccgccc gcatccctgc
     4981 tcgcatcgtc tctggaacac tgctgcgaac atcgtagaag acgtttctta gaaacggatt
     5041 agagtgcgca tgtctcacca cgcgacgcat atccgagcgt cgctatcttc gacgggcgag
     5101 actaccttta ccgatggtaa gcactgatgg taaaactacc gctgagggaa tggcggagcg
     5161 agacagacct ccgccttaga ggctgaaagg ggagtagcgt atgcagcaga gcggagtaga
     5221 tcaggagttc cggccgccgg tgccgccgtt cgacgaggcg tcggcgcgcg agaaggtcaa
     5281 gaaggcgcag gacgcctgga acacccgcga tcccgagaag gtcgccctcg cctacacgcc
     5341 ggactcgaag tggcgcaacc gcgacgagtt cttcgagggc cgcgaggcga tagtggagtt
     5401 cctgaagcgc aagtgggcca gggagctcga ctaccggctg atgaaggagc tgtggtgcta
     5461 ccaggggaac cgcatctcgg tgcgctttga gtacgagagc cgggacgtgg acgggcagtg
     5521 gtggcgcagc cacggcaacg agcactggga gttcgatgag cggggtctca tgcgccgccg
     5581 cgacgcctcg ataaacgact acaagataga ggagtcggac cgccggatct tctagcgggg
     5641 gccggacggt ggtctgctag acttcgctgc gtaaacaaac gaaaggacag tctgtgatcg
     5701 accatctcgt actgattgca gtccgtgacg attgctcgcc ggaggaagtc gccgacctcg
     5761 tggactccgc caggaagctg aaggactcgg tgccgggcgt cgtggacctc tccataggcg
     5821 aggacttcag cgggcggggc ggcgagtaca cgcacggtct cttcgcccgc ttcgaggacc
     5881 gggacggtct tcaggggtac ctcaagcacc cggatcacat cgcggtcgtc gaaaagctcg
     5941 acaggtacac gaacggcagg ctcgtcctgg actacgaggc gtagccggcg ggggagagtc
     6001 gtttcatctg gaatagagta gtttgcgcgt aaaccgtact caggggctcg tgctcggctt
     6061 cttcgccttc gtctgggtct ccctggtagt gatcctcatg acggcgcccg agatctacga
     6121 tcaggccctg cgaatgtcgg cgggcggcca tcgtgcgttc gagatagcgt tcctggccgc
     6181 aatctccgcc ttcatcgcgc tgctgagcgt cgggaccgtc cgccggtggc gctggacgtt
     6241 ctggctgatc ttcgttgctt ttatattcgg gttgctgcgc gtgccggcgt ccatcctgca
     6301 actgataggg cttctgcgcg cgcaggggcc ggtatggtac acactgtttc aggccgcgct
     6361 cggtctggtc cagttcgcca tagcgctggc gatgctggcg gggtatcggc ggaacggcgt
     6421 ctggggaagc ttcgggagcc ccgagtagag gaggatcttt ggaatacctc aagatcacac
     6481 cgggtgaact gaagtcccgg ctggactccg gcgagcggcc cgtgcttctc gacgtgcgcg
     6541 agccgtggga gtatgaagcc gcccgcatag aaggctccag gctcatcccg ctcggagaga
     6601 tgccccaccg cgtctccgag ctggaccccg cgagggagac cgtcgtcatc tgccaccacg
     6661 gcgcccgcag cgcatatgtg gcgcaggccc tcacgcggtc cggcttctcg aaggtcctga
     6721 acctggaagg tggtctcgac gcctactccc gggtggacgc ctcggtgccg aggtactgag
     6781 cggtcatcca tgagtagccg gaccggctga taggaggagg ccggaccgag ttagccggcc
     6841 ttgacgatct cttctacacg ccggtagagc ggggcggcca ggccctcggg gtcggcggag
     6901 tgttcggcca gggcgcgggc ggcctctcgg gcggtggcgt cgccggggac ctcgaacgtc
     6961 aggaccacgg ggtccgtgtc ctccagcccc agctcttcac ggaggacctg cataacctct
     7021 gtcgggctgc ggccggtact ctcggctatc tcgcggctca tcctttccgg gtccagctcc
     7081 tggcagagtt cgatgccgcc ggagatccgg cgttcccacc accatcgggc gctcacgccg
     7141 gtaaaactct ctttcaccag cgccgccgcg gggtccgcca gcagctctcg tatctgatct
     7201 cccacgaccg cgttatacca gtttctgcgg atctccgggc agcccttacg ggaagcgtcc
     7261 gggtcatata gaattcatgc agggagataa ggaaaggtac tgcatgctgc tggtcaggga
     7321 ctccatgacc cgtgaggtcg ttacgctcac gccgcagacg acggcggcgg aggcgcttgc
     7381 gttgtgccgg gaccggagga taagacatct gccggtgctg gagaacgggc ggctggccgg
     7441 gatggtctcc gaccgggacc tgcgttcggc gacaccggcg ctcggggacc cggcgcgggc
     7501 cggggcgctg gagaggataa aggtcgggga cgtgatgtcg cgggaggtct ccacgtcgca
     7561 ccccgacgat cccatagagc aggcggcgaa cgagatgcgg gagaagaaga tcggctgcct
     7621 gcccgtggtc gagcacggta cgctggtcgg gatagtaacc tcctcggacg taatggaagc
     7681 gctgacctac ctggttggcg cgcacgagcc cggcagccgc atggaggtct ccgtccccga
     7741 ccgtcccgga actctcgccg gggccgcggg catcttcggg atgtgcggca tcaacatcgt
     7801 cagcgtcgtt atgggcgcca ggacggagcc cgcctcgaag gacgcgctgc cggagcgcct
     7861 ggtcgtcttc cgagtagaca ccatagacac gagtgaggta gcccagtacc tggaggatgc
     7921 cggctacagc gtgctatggc cgccccggaa atccgcgtga cggagactgt ctcctgagcg
     7981 ggaagtccgc cattgtccac gaccggcggc tggagagcta cgggttcggg gacgaccatc
     8041 cgtttaaccc tctccgcatc cgcatgacgc tggagctctg cgagtcgctg ggcctgttcg
     8101 acggttacga gttcgtgggg tccgagcccg cgagcgacga ggacttgagg acggtccaca
     8161 gcctgaccta catccgcagc gtccagcagg cggggcgtgg tctggccgac ctggcggacc
     8221 tcatttcata cggcatcggg accccggaca accccatcgt tccggacatc cacgaggccg
     8281 gggccgacgt gtgcggcgcg gtcctcaagg cggcccgcat ggtgataagt ggggaggtgg
     8341 atcacgccct gtgcatctcc gggggcctgc accacgcgct gcgctcgcag gcgagcgggt
     8401 tctgcttcta caacgacgcc ggggtcgcca tcgcccggct caagcaggag catcccggaa
     8461 tccgcgtcgc ctacctggac accgacgcgc accacggcga cggagtgcag tggatgttct
     8521 acgatgaccc ggaggtactc accatctcca tgcacgagtc cggccggtat ctcttccccg
     8581 gcaccggggg ggtggaggag aggggccgca acggcggggt gggctactcc gtgaacgtgc
     8641 cgctggagcc gttcacggac gactcgtcct ggatctcctg cttcgaggcc gtcgttcccg
     8701 aggcgttgcg gacgttcggg ccggacctga tcctctctca gaacggctgc gacgggcaca
     8761 agctcgatcc cctgacccac ctctccgcga ccacccgcct ctacgagcac gtcccgcgcc
     8821 gcgtccacga gctcgcccac gaactgtgcg agggccgctg ggtcgccacg ggcggcggcg
     8881 gatacgacat ctggcgcgtc gtcccgcgag cctggacaat actctgggcc accctctctc
     8941 accagcggat cccggagaaa gtctccggcg aatggttaga caagtggggc gcgcggagcc
     9001 ccgtccggct cccgacgctg atgcgtgacg acccggcaga ctaccctgag atctcacgcg
     9061 ccgaggagat agccgaacgc aacagccgga ccgtcgcgca cgtgctggag agcgtgctgg
     9121 cgcgcataaa agcataagag aaaaacaaga gaggagttgc gccatcctct caagacggct
     9181 atctttctgg cgttggcgaa cgcgacggac cgaaggtgtg tatgcccgca atgctagaga
     9241 tactcggtgc ttccgggacc gagaccgctc ccaaagagac gacccggcga aagatgacca
     9301 ccgagccgcc ctacaaggtc atcctgcaca acgacgacta cacgccgatg gaacacgtca
     9361 tacaggtgct ccggaaggtc atcccgcgca tgagcctgcg ccgcgccgtc tcgat
//
LOCUS       DASSMB010000046         8729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_11185, whole genome shotgun sequence.
ACCESSION   DASSMB010000046 DASSMB010000000
VERSION     DASSMB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8729
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11185"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VFJ72_03255"
     CDS             complement(<1..794)
                     /locus_tag="VFJ72_03255"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET7478518.1"
                     /translation="MADVFVVGSINQDLVLKVERRPEPGETVTDAELSTHNGGKGANQ
                     AAAAALLGASVAFLGRVGDDEFGGPLVRALAEKGVDMSLVERDSGTVTGTAFITVTPD
                     GENAIVVAPGANRRLTPEDVDAASDDIRQARVLVAQMEVPLESVLRAAEIADAGGTRV
                     VLNLAPPREVPSALLEKLDPLVVNEHEAAFMLGTKVEGQEGARAASRELLALGPRSVV
                     ITLGPAGALVADRETVTHVPAPEVEVVDTTGAGDAFVGALVARLARG"
     gene            complement(805..2388)
                     /gene="mdlC"
                     /locus_tag="VFJ72_03260"
     CDS             complement(805..2388)
                     /gene="mdlC"
                     /locus_tag="VFJ72_03260"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HET7478519.1"
                     /translation="MSTVREVTFDLFRRLGTTTIFGNPGSTELPFLKDFPDDFRYVLA
                     LQEASALGMAEGFAQGTGRAAIVNLHTGPGLGNAMGAMVTAWHNKTPLLVTAGQQDRR
                     HSALEPLLMGKLVDLARPYVKRSFEPVRAEDVPGEILRAYHTAMMPPRGPVFLSIPMD
                     DWDAEAEPLEERAVVYRMAPDPDGLGKMAGILEEAQNPAMVVGAGVERAGAFYDAVAL
                     AEKLRSPVWQDPISPFASFPQDHPLFQGHLAPAQKHLAAQLEGHDVVLVLGAPVFLYY
                     PYVPGPTIPDGTRVLQITEDPDEASRAAVGDSLVGDVGIAIQTLAELLPGTDRPAPPR
                     REAPPAPEASDPMSVKYLMHAISEVVPDDVAVFDESASSKMTLHKHVLLKSPGGYHSS
                     GAGGLGFCMPGSVGYKLAVPERTVVCVIGDGSSMYSIQALWTAAQYGANVVFIVVNNR
                     GYSILKGFRDSIGAGDKVPGLDVPDVDFVSVAKGLGVEAERVAAAEDLRPTLKRALSA
                     ERPYLLEVVVDPAVPKLLS"
     gene            complement(2484..3119)
                     /locus_tag="VFJ72_03265"
     CDS             complement(2484..3119)
                     /locus_tag="VFJ72_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478520.1"
                     /translation="MDLASGAQILSSFAVLLLAIFAFFQVAEARAARIAQDSPQILVD
                     VNYSQRTMINIVVRNIGRGAAREIRFHFSAPIESSGGQVISDLPYFKEGIAFLAPNTE
                     IATVWDSYASAVEVIKAKGLRNGITVTARYKDLRGKPYSTGWTLNPLILEGSGYADYR
                     EFEDQVRALEKISRDLEAIKEALIRWEGPHDASRTPDREVGVDGVGDAASR"
     gene            complement(3256..4875)
                     /gene="pknB"
                     /locus_tag="VFJ72_03270"
     CDS             complement(3256..4875)
                     /gene="pknB"
                     /locus_tag="VFJ72_03270"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET7478521.1"
                     /translation="MQGTILDKRYTLLDRLGSGGMAEVYLAHDEVLDRDVALKALREQ
                     YADDDSFVERFRREARNAASLSHPNIVQVYDQGRAENGTYYMAMEYVPGGTLKDRIVE
                     HGALEPREAAGLAAQVAEALAVAHDRGIVHRDIKPQNVLMTPSGETKVADFGIARAAS
                     ATVMTQTNLVLGTAGYMAPEQAAGEEVSPATDLYSLGVVLYEMLTGALPYEADTPLAV
                     AMKHVNAPLQHPRDANPEVPQELDAVVVRLLAKDPSERYASARELARDLRRMAAGSGA
                     GLSAAATTVQAPRAATQQTRVAPAPARATVRRRRGLPVLLGLLALLLLLGAGWTLSQG
                     FGNSGGGEVAVPDVVGLKEDAARQKLTDAGLDASVSKQESSADKEGEVLEQSVAAGKK
                     VQEGSSVGLTVGEGTSKVAVPDIPYGATPDQARSQLEEAGLTLGSQSQTPSDQVSAGG
                     VVAQDPLPGVQVEKGTAVNITLSSGPAQAPAPEPTVAAPQQSGPSQQSDSSQQGSPNS
                     GDQNGSQPDVPSADDLQKQIQKQIKGKGKGN"
     gene            5014..5985
                     /locus_tag="VFJ72_03275"
     CDS             5014..5985
                     /locus_tag="VFJ72_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HET7478522.1"
                     /translation="MKAIRVHEFGGPEVLSYEDVEVPEPGPGQARVKLAASGVNFIDV
                     YQRTGIYPGELPRTLGLEGAGEVEAVGEGVEDLSAGDYVAFAMTPGSYAEQVVVPADK
                     LVPFNVTLVEARVAAAVMLQGMTAHYLTHSTFPLEEGQTALVHAAAGGVGLLLCQIAK
                     MKGARVIGTVSTEEKAALAREAGADEVILYTEQDFVEETRRITDGEGVHVVYDSVGKT
                     TFDGSLDCLRTRGYMVLFGGSSGQVPPFDLQILNQKGGLFVTRPSLAQYTTTREELMW
                     RAESLFSWIGQGNLNVRIGGTYSLEDTAQAHRDLEGRKTTGKLILIP"
     gene            6012..7037
                     /locus_tag="VFJ72_03280"
     CDS             6012..7037
                     /locus_tag="VFJ72_03280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET7478523.1"
                     /translation="MKLDMGLGTEGKYLKDVEKTARAAEGLGFAGLWTSETKHDGFLP
                     LAIAANATERMELGTSVAIAFSRSPMEIAQTAWDLQDISDGRFILGLGTQVKAHVTRR
                     FSMPWDKPTARLREYILAVRAIWESFQSEGSLQFEGEFYRHTLITPFFNPGPIEHPNV
                     PVYIAGVNTRLAHLAGEICDGFHVHPFHSPEYIRKTVQPAIADGAKEAGRDPGNVELA
                     TSVFVIAGRDDDDAAKQRESVRAQISFYASTPTYRTVLEAHGWEEVGERLGTMAREKK
                     WREMPSLITDEMLTAFAVEAAPDEVGPALKERYEGLIERVALYIPFVPGERDDFWRAT
                     VESVHAA"
     gene            complement(7046..7534)
                     /locus_tag="VFJ72_03285"
     CDS             complement(7046..7534)
                     /locus_tag="VFJ72_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7478524.1"
                     /translation="MGSGDSVFVGPNDGREIVNPIGGRMVVKVRDGDTAGAYSVHDNV
                     IPPGSPGPRPHLHRDFEETFYVLEGELTVRVGPRKVTAPAGSFVVIPRGVVHRPSNPG
                     TQPTRVLLIFSPAGMDHFFEEAAERRMPLQAAPEDPAVQEALAAFTEKYGYEFAELPP
                     EQ"
     gene            complement(7632..8600)
                     /locus_tag="VFJ72_03290"
     CDS             complement(7632..8600)
                     /locus_tag="VFJ72_03290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7478525.1"
                     /translation="MAAGEGKGAEEQGTRKSVFAAFTANLLITISKFVAGFVTGSAVL
                     LAEGAHSIADTINQVFLLVSLRSASEAPDDEHPFGHGKDRFFWSLLVAVGLFVAGAVF
                     SFYEGINKILSESEGHSSFLAGYIVLAAAFVFEGSALAVSAREFRRTAREAGHSFWNH
                     FRTTRNTTMKVPLYEDVAALIGILIAATGLLLTRTTGSPVFDGAASIGIGVVLIFVAW
                     ELGTDSRRLLIGEAMPKESRDKIEETMLSFSEVTEVLRLLTMHLGPNSALLTAEIHVQ
                     DGLDTDQIEDLLERITQKICAEVPEVTQTFIELHPEGETGGPSLTG"
BASE COUNT         1450 a         2970 c         2819 g         1490 t
ORIGIN      
        1 ccgcgtgcca gccgggcgac caacgccccg acgaatgcgt cccccgcgcc cgtcgtgtcc
       61 acgacctcga cctcgggagc cgggacatgc gtgacggtct ctctgtcggc gacgagcgct
      121 cccgcggggc cgagggttat aacgacggag cgcggtccga gggcgaggag ttctcttgac
      181 gccgcacgcg ctccttcctg gccttcgacc ttcgtaccga gcatgaaggc ggcctcatgc
      241 tcgttcacca ccagaggatc gagcttctca aggagcgccg acggcacctc tcgtggcggg
      301 gccaggttca agacgacacg agtcccgccc gcgtcggcta tctcggcggc tcgcaggacg
      361 ctctcaagtg gcacctccat ctgggcgacc agcacgcgag cttgccgtat atcgtccgag
      421 gcagcgtcca catcctcggg ggtgagacgc cggttggcgc ccggagccac gacgatggca
      481 ttctcgccgt ccggagtcac ggtgataaag gccgtgccgg ttaccgtgcc ggagtcccgc
      541 tcgaccaggc tcatgtccac acccttctca gccagcgccc gcaccagcgg cccgccgaac
      601 tcatcgtctc ctacccggcc caggaacgcg acggaggcgc cgaggagagc cgcggcggcg
      661 gcctggttcg cgcccttgcc gccgttgtgg gtggagagct cggcgtccgt aacggtctct
      721 cccggctccg gacggcgctc gaccttcaag acgaggtcct ggtttatgga ccccacgacg
      781 aatacgtcag ccatctccgg ctacctaaga aaggagctta gggacggccg ggtccacaac
      841 cacttccaga aggtacgggc gttcggcgga gagggcgcgc ttgagggttg gccgaagatc
      901 ctcggcggcg gcaaccctct ctgcttctac accgagacct ttagccacgg agacgaagtc
      961 cacgtccggg acgtctaggc cgggtacttt atctccggcg ccgatggaat cgcgaaagcc
     1021 cttgaggatc gagtatccgc ggttgttgac tacgatgaag acgacgttcg ctccgtactg
     1081 ggcagcagtc cagagggcct gtatggagta catgctcgac ccatcgccga tgacgcagac
     1141 cacagtgcgc tccgggacgg ccaacttgta tccgacagag ccgggcatgc agaacccgag
     1201 accgccggcc ccggagctat ggtagccgcc gggactcttt agcaggacat gcttgtggag
     1261 cgtcatcttg ctggacgcgg actcatcgaa gaccgccaca tcgtccggca cgacctccga
     1321 gatggcgtgc atcaggtact tcacggacat ggggtccgaa gcctccggcg ccggaggagc
     1381 ttcccggcgg ggaggcgcgg gccggtcggt accgggcagg agttccgcga gggtctgtat
     1441 cgcgataccc acgtccccga ccaggctatc cccaacggcg gcgcgggacg cttcgtccgg
     1501 gtcttcggtg atctggagta cccgcgtccc atcggggatg gtgggaccgg ggacgtatgg
     1561 atagtagagg aagaccggcg ctccgaggac aaggacgacg tcgtggcctt cgagttgggc
     1621 ggcgaggtgt ttctgggcgg gagcaaggtg tccctgaaag agcgggtggt cttgcgggaa
     1681 gctggcgaac ggggagatcg ggtcctgcca caccggcgac cgcagcttct ccgcgagcgc
     1741 caccgcgtcg tagaacgctc cggcccgctc caccccggcc ccaacgacca tcgcagggtt
     1801 ttgcgcctcc tctagaatcc cggccatctt ccctagcccg tccgggtccg gggccatgcg
     1861 gtagacgacg gcacgctctt ccagaggctc ggcctcggcg tcccagtcgt ccatcgggat
     1921 cgagagaaag acgggaccgc gcggcggcat catcgcggtg tggtaggcgc gcaggatctc
     1981 acctggcacg tcctcggcgc gaaccggctc gaagctgcgc ttgacgtatg gcctggcgag
     2041 gtccacgagc ttgcccatca gcaagggttc gagggcgctg tggcgccggt cctgctgtcc
     2101 ggcggtaacg aggagcgggg tcttattgtg ccaggccgta accatcgcgc ccatcgcgtt
     2161 gccgaggccc ggcccggtgt gcaggttgac tatcgcggcc cgccccgtgc cctgagcgaa
     2221 cccctcggcc atgccgagcg ccgacgcctc ctggagcgcc aggacgtatc ggaagtcgtc
     2281 cgggaagtcc ttcaggaacg gcaactcggt ggagccgggg ttgccgaaga tcgtcgtcgt
     2341 cccgagcctg cgaaagaggt cgaaagtgac ctcacggacc gtagacatag cgcaccgccc
     2401 cttcctggta agcctcgccc tccgcgcaaa tatagcagag aagcagtccg gcgcacttct
     2461 cgcttgcgag agtcttgcgc ggcttaccga ctagccgcgt ctccaacgcc atcgacgccg
     2521 acctcccggt ccggtgtccg cgacgcatca tgcggtcctt cccagcgtat tagtgcttcc
     2581 ttgatggctt ccaggtctcg ggagatcttc tcaagggctc ttacctgatc ctcgaactct
     2641 ctgtagtcgg cgtatccgct accttcgagg atcagcgggt tgagcgtcca cccggtagag
     2701 tagggtttcc cgcgcagatc cttatacctc gccgtcaccg tgatcccgtt tctgagtccc
     2761 ttcgccttta tcacctccac ggccgaggcg taagagtccc agacggtggc gatctcggta
     2821 ttcggcgcca ggaacgctat gccctctttg aagtacggca ggtcggagat gacctgtccg
     2881 ccggagctct cgatgggcgc cgagaagtgg aacctgatct cccttgccgc ccccctcccg
     2941 atgttgcgga cgacgatgtt gatcatggtg cgctggctgt agttcacgtc caccagtatc
     3001 tgcgggctgt cctgggctat gcgggccgcg cgggcctccg ccacctggaa gaaagcgaag
     3061 atcgccaaca ggagcacggc gaaagaagac agaatctgcg cgcccgatgc caggtccatt
     3121 tctcgctccc ttgtaggtag agggcctgca tgctccgggc tcttaccagt aatacgatct
     3181 ccgccgccaa cggccacgtt gtccgcggcg cggaaggcgt ggagggccgg ctgccggttc
     3241 cggcgggctg tgtggctagt tgcctttgcc cttgcccttg atctgcttct ggatctgctt
     3301 ctgtaggtcg tcggccgacg gaacgtccgg ctgcgaaccg ttctgatctc ccgaattcgg
     3361 agaaccttgt tgcgacgagt cggactgctg cgaggggccg gattgctgcg gagccgcgac
     3421 ggtcggctcg ggagccgggg cctgcgcggg accggaactc agcgttatgt tcacggcagt
     3481 ccccttctcc acctggactc cgggcagggg gtcctgcgcc acgacgccac cggccgagac
     3541 ctgatcgctc ggcgtctggc tctggctgcc gagcgtcagg ccggcttctt ctaactggct
     3601 tctcgcctga tcgggggtcg cgccgtacgg gatgtccggg accgcgacct ttgatgtccc
     3661 ttccccgacc gtcagaccca cgctggagcc ctcctgcacc ttcttcccgg ccgccaccga
     3721 ctgctccagg acttctccct ccttgtcggc ggaactctcc tgcttggaca cgctggcgtc
     3781 gagtccggca tccgtcagct tctggcgcgc ggcgtcttcc ttcaggccga cgacgtccgg
     3841 gaccgcgacc tcgccccctc ccgagtttcc gaagccctgc gagagtgtcc agccggcgcc
     3901 gagcagtagc agcagcgcga gcagcccgag aagcaccggc agacctctcc gccgccggac
     3961 cgtagctctg gccggcgcgg gagcgacgcg cgtctgctgc gtcgcggccc gaggagcctg
     4021 cacggtggtt gcagccgcgg agagcccggc gccgctgccc gcagccatgc gccgcaagtc
     4081 gcgggccagt tctctggcgc tcgcgtagcg ctcgctcgga tctttggcga gcagacgcac
     4141 gactaccgcg tccagctcct gcgggacctc cgggttcgcg tcgcgcgggt gctggagcgg
     4201 cgcgttgacg tgcttcattg cgaccgcgag gggggtatcc gcctcgtaag gtagcgcccc
     4261 ggtgagcatc tcgtacagga cgacgcccag agagtagagg tcggtggcgg gacttacttc
     4321 ctctcccgcg gcctgctccg gcgccatgta tcccgcggtc ccgagcacca ggttggtctg
     4381 cgtcatgacc gtcgccgacg cggcccgcgc gatgccgaag tccgccacct tcgtctcacc
     4441 ggaaggcgtc atcaggacgt tctgcggctt tatgtcccgg tgtacgatgc cccggtcgtg
     4501 ggcgaccgcc agcgcctcgg ccacctgcgc cgcgagcccc gccgcctcgc gaggctccag
     4561 cgcgccgtgc tccacgatgc ggtccttcaa cgtcccgccg ggaacgtact ccatcgccat
     4621 gtaataagtg ccgttctcgg cgcggccctg atcgtagacc tgcacgatat tcgggtgcga
     4681 gagcgaggca gcgttgcgcg cctcgcgccg gaagcgctcc acgaagctat cgtcatccgc
     4741 gtactgctcg cgcagcgcct tgagggccac gtcgcggtcg aggacctcgt cgtgagccag
     4801 gtatacttcg gccattcctc cgctgcccag ccggtccagc agggtgtagc gcttgtccag
     4861 tatcgttccc tgcatggctg cattctaccg ttgtatccgc gcgtatgaaa cccgcgtact
     4921 cgaccgcgtc ccccggaacc ttcctcggag aacgcaatcc cggctaaaat ggtctcctga
     4981 acgggtggag tctccgagcg cagggggtaa tggatgaaag cgatacgggt tcacgaattc
     5041 ggcggcccgg aggtcctgag ctacgaggac gttgaagtcc cggagccggg gccgggacag
     5101 gcgcgggtaa agctcgcggc ctccggcgtc aacttcatcg acgtctatca gcgtaccggc
     5161 atctatcccg gcgagctgcc ccgtactctg ggcctcgaag gcgcggggga ggtcgaggcg
     5221 gtcggcgagg gtgtcgagga cctatcggcc ggtgattacg tggctttcgc gatgacgccc
     5281 ggctcctacg ccgaacaggt cgtagtcccg gcagataagc tcgttccgtt caacgtaacg
     5341 ctcgtggagg cgcgggtcgc ggcggcggtg atgctccagg gcatgacggc ccactacctg
     5401 acccacagca cgttccccct cgaagaaggt cagacagccc tcgtccacgc ggcggccggc
     5461 ggtgtcgggc tcctgctgtg ccagatagcc aagatgaagg gcgcgcgggt catcgggacg
     5521 gtctcgaccg aggagaaggc agctctagcg cgcgaggccg gggcggacga ggtgatcctc
     5581 tacaccgagc aggacttcgt ggaggagacc agacgcatca cggacggcga gggcgtccac
     5641 gtcgtctacg actccgtcgg caagaccacc ttcgacggca gcctcgactg cctcaggacg
     5701 cgcggctaca tggtcctctt cggcggttcc agcggacagg tcccgccgtt cgacctgcag
     5761 atcctgaacc agaagggcgg cctcttcgtc acccggccct cgctcgccca gtacaccacc
     5821 acccgcgagg agctgatgtg gcgcgccgag agcctgttct cctggatcgg gcagggaaac
     5881 ctcaatgtcc gcatcggcgg gacctactcg ctggaggaca ccgcgcaggc ccaccgcgac
     5941 ctcgaaggcc gcaagactac cggtaagctc atcctgatcc cgtaaaaccc gtaggacctg
     6001 taggaggcgc tatgaagctg gacatgggac tgggcaccga gggcaagtac ctcaaggacg
     6061 tggagaagac cgcccgcgcc gccgaagggc tcggtttcgc ggggctgtgg accagcgaga
     6121 ccaagcacga cggcttcctc cccctcgcca ttgccgccaa cgcgacggag cgcatggagc
     6181 tgggcacctc cgtagccatc gccttctccc gctccccgat ggagatagcc cagaccgcct
     6241 gggacctgca agacatctcc gacgggcgct tcatcctcgg cctcggcacc caggtaaagg
     6301 cccacgtcac gcgccggttc tcgatgccct gggacaagcc gaccgcgcgg ctgcgcgagt
     6361 acatcctggc cgtgcgggcc atctgggagt cgttccagag cgagggctca ctccagttcg
     6421 agggcgagtt ctaccggcac acgctcatca cgccgttctt caaccccggt cccatcgagc
     6481 accccaatgt cccggtctac atcgccggcg tcaacacccg gctcgcgcat ctggccggag
     6541 agatctgcga cggtttccac gtccacccgt tccacagccc cgaatacatc cgcaagaccg
     6601 tgcagcccgc catcgccgac ggcgcgaagg aagccggccg agatccaggc aacgtggagc
     6661 tggcgacgag cgtcttcgtc atagcgggcc gggacgacga tgacgccgcg aagcagcgag
     6721 agtccgtgcg ggcccagatc tccttctacg cctccacccc cacctaccga accgtcctcg
     6781 aagcccacgg ctgggaagag gtcggcgaga ggctcgggac gatggcgcgg gagaagaagt
     6841 ggcgcgagat gccatccctc atcaccgacg agatgctaac cgccttcgcc gtcgaagccg
     6901 cgcccgacga ggtcggccct gccctgaagg agcgctacga gggtcttata gagcgggtgg
     6961 cgctctacat cccgttcgtc cccggcgaga gggacgactt ctggcgcgcg acggtggagt
     7021 ccgtgcacgc cgcgtagggc cgtagttact gctccggcgg caactccgcg aactcgtaac
     7081 cgtacttttc ggtaaaggct gcgagcgcct cctgtacggc ggggtcttcc ggcgccgcct
     7141 gcagcggcat gcgccgctcc gccgcctcct cgaagaagtg gtccatcccg gccggggaga
     7201 agatgagcag gaccctcgtc ggctgcgtcc cagggttcga gggccgatgc acgacgcccc
     7261 gcggtatcac cacgaacgaa cccgctggag ccgtgacctt gcgcggccca accctcaccg
     7321 taagctcgcc ctccaggacg tagaaggtct cctcgaaatc gcggtgcagg tgaggccggg
     7381 ggccgggaga gcccggcggg atgacgttgt cgtggaccga gtacgccccg gcggtgtccc
     7441 cgtcccggac cttgaccacc atccggccgc cgatggggtt cacgatctcc ctgccgtcgt
     7501 tcggaccgac gaagacacta tccccactgc ccatatccgc gctccttccc gctcaacagc
     7561 cgacgaatgg gccttgccac gttcagtata acggcatggt gaagatcaga cccaggcagg
     7621 ccgcgtgcgc gttagcctgt cagtgaggga ccgccggtct cgccctcggg atgaagctct
     7681 ataaaagtct gggtaacttc ggggacttcg gcacagatct tctgggtgat gcgttcgagg
     7741 aggtcttcga tctggtctgt gtccaggccg tcctggacgt ggatctccgc ggtcagcaac
     7801 gcggagttgg gcccgaggtg catggtcagc agacgcaaga cctccgtgac ctcggagaag
     7861 gagagcatgg tctcctcgat cttgtcccgt gactccttcg gcatcgcctc cccgatgagg
     7921 agcctccgcg agtccgtccc caactcccac gccacgaaga tgagcacgac cccgatgccg
     7981 atggaggccg cgccgtcgaa gaccggactg ccggtggtcc gggtcaggag cagccccgtc
     8041 gccgcgataa gaataccgat cagagccgcc acgtcctcgt agagcggcac cttcatcgtg
     8101 gtgttgcggg tggtcctgaa gtggttccag aacgagtgcc cggcctccct cgccgtccgc
     8161 cggaactcgc gcgcgctgac ggcgagcgcg cttccctcaa agacgaacgc cgcagccagc
     8221 acgatgtaac cggcaaggaa cgagctgtgc ccctcggact cgctgaggat cttgttgatg
     8281 ccctcgtaga atgagaagac cgcaccggca acgaagagac ctacggcgac cagcagggac
     8341 cagaagaaac ggtccttgcc gtggccgaag gggtgctcgt cgtccggcgc ctcgctcgcc
     8401 gaacgcaggc tgaccagcag gaagacctgg ttgatggtgt ccgcaatgga gtgggcgccc
     8461 tccgccagca ggaccgcgct cccggtaaca aagccggcca cgaacttgga gatcgtgatc
     8521 aggaggttcg ccgtgaacgc cgcgaagacg ctcttcctgg tcccctgctc ctcggcgccc
     8581 ttcccctccc cggctgccac gcgcctaccg cgctacggct tcaggacgca cttgatcgcg
     8641 ccgtcctgct tcttctggaa gatttcataa gcgcgcggcg cctcttcgag cggcatctcg
     8701 tgcgtcttga ggtcttcggt ccccagcgg
//
LOCUS       DASSMB010000047        18214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1533405, whole genome shotgun sequence.
ACCESSION   DASSMB010000047 DASSMB010000000
VERSION     DASSMB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533405"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VFJ72_03295"
     CDS             complement(<1..542)
                     /locus_tag="VFJ72_03295"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET7478526.1"
                     /translation="MTEAAIQERPITSRQITVGGVPIGGGAQIAVQSMTNTMTYDVEA
                     TVQQVYDLNAAGADLVRVSVNGSKALKGFREVVWRSPIPLIADIHFDYRMALGAADAG
                     AACVRINPGNVGSDDRYRQVIEKCQETGVAMRIGVNSGSVEKKYWDLPKAEALVASAL
                     HKVEIAEEMGFFNFKVSLKA"
     gene            complement(573..1613)
                     /gene="rseP"
                     /locus_tag="VFJ72_03300"
     CDS             complement(573..1613)
                     /gene="rseP"
                     /locus_tag="VFJ72_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET7478527.1"
                     /translation="MTVIVALLGLILLIVIHELGHMLTAKALGVRVPEFGIGFGPALF
                     KKKIGKTVYSFRVVLLGGFAKMAGMNDDARGPDTYPAKAAWRRALIIFAGPFANIIAA
                     IAILAGTLMISGVVVGTPVVDKVERGTMADRVGIQAGDRVVAVDGQRIQKWEGFTRAV
                     RDKKPGDEVSVTVKRDGAEKQFSGKLGESKQYPGQALVGVRPEVRQITYGPLEATGMA
                     AQKIWEITVKMVVFIGQLITGQQDFYKNVTGPVGIASVGSTAVSQGFFPQLLAVISLN
                     LAIFNLLPVLPLDGGHLFFIVMEKILGRPVSQETMGKVAALGIALVLVLFVFATYADL
                     SKLFTGQSFIPN"
     gene            complement(1610..2746)
                     /gene="dxr"
                     /locus_tag="VFJ72_03305"
     CDS             complement(1610..2746)
                     /gene="dxr"
                     /locus_tag="VFJ72_03305"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: protein motif:HMM:TIGR00243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET7478528.1"
                     /translation="MTRRLTILGSTGSVGQQALDVVRAHPDRFEVVGLSAASNADLLR
                     AQAAEFNPEYVALEKGEKDALSGCAAETLVGPGSARALAAAPADVVLNGIVGFAGLSA
                     TVAALQAGNQVALANKESLVAGGRWIMDLARDNPILPVDSEHSAIFQCLEGRDEAEVA
                     EVLLTASGGPFYEFEKGRLMEARPEDALKHPTWNMGAKITIDSATMMNKGLEVIEAHH
                     LFGTSYDDVRVVVHRQSVVHGGAIFRDGSVIMHAALPDMRLPIAFGILYPERVDVGAE
                     PVGFGEMTWTFQEPRNDVFRCLPLAVEAGRAGEAYPVALNAANEVAVEAFLERRIQFL
                     QIADLIEEVLELVPDFGTMRSMEAIEGVDRWARDEAARLVERAR"
     gene            complement(2743..3537)
                     /locus_tag="VFJ72_03310"
     CDS             complement(2743..3537)
                     /locus_tag="VFJ72_03310"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7478529.1"
                     /translation="MLRWRTVTALVLAPIALGAILLGTWAVLLLVLLVVGLSAYELSR
                     ALNPLPFPAALGAGVIPILLSIPYAETGILAGALLSLPWALFWLAGRPEARTLRAVLA
                     ILLMALWVGASLAHLGLISQLQDRIPLILIAVVGPWISDSGAYFAGRLFGRHKLFPSL
                     SPNKTLEGSIGGLMLTTAAVGWVANVFLGLPLPFAALMGAGISVFSQSGDLFESILKR
                     ILELKDLGRALPGHGGILDRIDSLLFTGPAVYYIFVLSSLNTIHPI"
     gene            complement(3548..4228)
                     /gene="uppS"
                     /locus_tag="VFJ72_03315"
     CDS             complement(3548..4228)
                     /gene="uppS"
                     /locus_tag="VFJ72_03315"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET7478530.1"
                     /translation="MPRNVAIIMDGNGRWAQRRWLPRAAGHRAGVSVLTPLLEAAGEA
                     GVKTLTLYAFSTENWARPVDEVDTLMSLFLETARRKVPELNERGARLRFLGRREHLPE
                     SVQAAMLEAEDLTAHNDRLEVYIALNYGGRSEIVDAARRIVADGLTPEEVDEATFASY
                     LYAPEVPEVDLLIRTSGELRVSNFLLWQIAYAEFYITDTLWPDFSREEFFHAIESYAS
                     RSRRRGGV"
     gene            complement(4307..4867)
                     /gene="frr"
                     /locus_tag="VFJ72_03320"
     CDS             complement(4307..4867)
                     /gene="frr"
                     /locus_tag="VFJ72_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7478531.1"
                     /translation="MSDMIDLTAAERRMHGALESVRGHFATIRTGRANPAILDRVEVE
                     VYGSKMALRSVANINVPEPRLMTVTPFDPNSLKAIERAIEHSDVGLNPQNDGKILRLP
                     IPELTEERRRELIKVARHMAEEGRVSVRNVRRDEMRDIVELKKMGEISEDDERRAEQE
                     LQKLTNDYVKRIDAALSDKESELLEV"
     gene            complement(4871..5689)
                     /gene="pyrH"
                     /locus_tag="VFJ72_03325"
     CDS             complement(4871..5689)
                     /gene="pyrH"
                     /locus_tag="VFJ72_03325"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7478532.1"
                     /translation="MSQAAFKGGDEAPNAELGPVKRVVLKLSGESLAGEEGYGIDPVS
                     LQSTVSQVLEAVEGGAELAMVVGGGNIFRGSSVADELGIESATADYMGMLGTVMNAMA
                     LQAALEGRGVPTRVQSAVQIQEVAEPYIRRRAIRHLEKGRVVIFAAGTGNPFFSTDTG
                     AALRALEIGADALLMAKNQVDGIYDKDPRTHPDAVRVPRLNYMDLLSQNLAVMDHTAA
                     TLCSGENLPIVVFDILKPGNLRRILQGEHVGSLVWSARGRAADGGPTSDGATGE"
     gene            complement(5696..6283)
                     /gene="tsf"
                     /locus_tag="VFJ72_03330"
     CDS             complement(5696..6283)
                     /gene="tsf"
                     /locus_tag="VFJ72_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET7478533.1"
                     /translation="MASTIEKIKQLREETGAGMMDVKRAVTEANGDVEGARKLLRERG
                     QAQAQKKSGRATNEGLIEAYIHFNGRVGVMVEVDCETDFVARTGEFKEFARNVALHVA
                     SMNPLCVAPEDIPQDAMDEERAIAEKQAAEMGKPEKITRQIVDGRIKKWVAEQALLTQ
                     PFVKEPEKTVGDLLQDTVQKVGENVVIRRFVRYEL"
     gene            complement(6289..7278)
                     /gene="rpsB"
                     /locus_tag="VFJ72_03335"
     CDS             complement(6289..7278)
                     /gene="rpsB"
                     /locus_tag="VFJ72_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7478534.1"
                     /translation="MESTAERVGVRELLEAGVHFGHQAKRWNPKMKRYIFAQRAGVHI
                     IDLDQTITLLEDALKFIRSIAEAGREVLFVGTKKQAQLTIAEQAMRSGQPYVAERYIG
                     GMLTNFQTIQPRIQHFVNLAARVEETPEENRTGREWFALNREYQKLRRNFAGITEMER
                     LPGAVFVVDPKREELLIKEANRLRIPVVALTDTNCDPEVIDYVIPGNDDAIRAINLIS
                     RLVADAVLAGKGETEVPAEDRPVPPVVEEALVSEEAARESSASANASANGTGAEPEPQ
                     QPEAPQPGASLAAPEATDPEIADPEVAGPEAANEEEAEAVTEAPAEGDETEEK"
     gene            complement(7427..8257)
                     /locus_tag="VFJ72_03340"
     CDS             complement(7427..8257)
                     /locus_tag="VFJ72_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HET7478535.1"
                     /translation="MRQTLGRLWARYLEVRGHLEEADRAESGKRDDLYREANQLRDRL
                     LVNYSPLVKYVAGRLGGRMTGAFDQEDFMSWGIMGLLDAIQTYEPDRGAKFESYAISK
                     IRWSILDELRREDRVPRRVRAHVREVEAARVRVTQELRRPPTEEELARALGVSVERHR
                     DMLDRYSQAMVGSLEARMELDNRAGSEYGDFVADSSASDPQSEAEKTEVRGRLVAALE
                     RLAERERVVATFYFYEGLTLKEIGMAMNLTEGRISQILRRALELLRADLSDGTAFPLG
                     "
     gene            complement(8254..9357)
                     /gene="dprA"
                     /locus_tag="VFJ72_03345"
     CDS             complement(8254..9357)
                     /gene="dprA"
                     /locus_tag="VFJ72_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET7478536.1"
                     /translation="MTARGRAAYLFLSLLQARVGTSVISRLGDLAPEAVPEMSSGELA
                     ARIKLTERARQAFDDLKRTFDAETVERRLSEKGIRAVTLADEDYPERLREVPDAPPAL
                     FVNGALPAGPAVAVVGSRKCSVSGIEVARELGRALGGRGVCVVSGLALGIDAAAHEGT
                     LMADGPTVGVLGCGIDVIYPRRNRDLYARVSTTGAVLSEYYLGEAPLAWRFPARNRII
                     AGLAETLVVVEAAEKSGALITARHALDSGRDVWAVPGPVGVAECRGSNKLLSEGAGVL
                     WDIREFVDAVAPVQETLPQRGEARSEDLPAGLPEREAAVLAGVGFEPTEVDVISSRSG
                     IGMRDLLPALTMLELKGYVGRNVSGAFVRRSTV"
     gene            complement(9347..10858)
                     /locus_tag="VFJ72_03350"
     CDS             complement(9347..10858)
                     /locus_tag="VFJ72_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HET7478537.1"
                     /translation="MSVCRVRSLTLAGIDALPVEVEVDVSAGLPGFSIVGLPDTAVQE
                     ARERVKVAISNSGYKFPTKKVIVNLAPAHLRKEGPAFDLPIALAVLAASGAMPAERLG
                     GAGLVGELSLDGGLRSIRGALSMAEGARREGMPRLILPEPNAAEAAAIGEVGVYGMRT
                     LREAVDFLGGRARMEPATASENGTVEVDLQDNDFADVAGQGYAKRALEVAAAGGHNVL
                     MSGPPGSGKTMLARRLPGILPPLTTRESIEVTKVHSAAGESRGSLIKSRPFRAPHHTI
                     STSGLAGGGSNPRPGEVSLAHHGVLFLDEFPEFSRGTLEVLRQPLEDGHVTISRVAAT
                     LSYPARVTLVCSMNPCPCGYAGDRRRACRCSAGQIERYQSRISGPLLDRLDLFVDVPR
                     LTREELRESHPAESSRRIRERVAAARRIQTERQGGPNSALSGRRLREVCAVGGDAEAL
                     LSRAIDRLGLSGRGHDRVLRVARTVADLSGERDIQVEHLAEALNYRRSSLIDR"
     gene            complement(10991..11383)
                     /locus_tag="VFJ72_03355"
     CDS             complement(10991..11383)
                     /locus_tag="VFJ72_03355"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET7478538.1"
                     /translation="MPGPQDELRRGRSLRTRSAGDWGEDLALRYLVRKGYTLVERNYR
                     TRYGELDLVVRDGDTLVFVEVKLRRGREFGHPTEAVTPRKQAAVRSMAEQYLAERDPE
                     FDELRFDVVGILASGHGPPEIQHLENAF"
     gene            complement(11341..11964)
                     /locus_tag="VFJ72_03360"
     CDS             complement(11341..11964)
                     /locus_tag="VFJ72_03360"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7478539.1"
                     /translation="MAGRNGGGRPSGGSLYAFDAAWLLERRGPLAGADEAGRGALAGP
                     IVAAAAILGEDEVLEVRDSKLLSAPVRERLLGEVLRCARAVSVVAFPAWWIDLHGLGL
                     ANREALRRAIALLDSHAGCGLADGNLRLGPEIECLPRADAASATVAAASIVAKVLRDH
                     AMRALADEYGRYGFERNRGYGTLEHRQALAEVGPCRVHRMSYAGVGP"
     gene            complement(11977..12303)
                     /gene="rplS"
                     /locus_tag="VFJ72_03365"
     CDS             complement(11977..12303)
                     /gene="rplS"
                     /locus_tag="VFJ72_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET7478540.1"
                     /translation="MITSENLQQKDVTFRPGDTVRVHYRVIEGNRERTQIFEGLCIAR
                     KGSGIGETFVVRKNSFGVAVERIFPLHSPKIAKLEVASRGAVRRAKLYYIRDKIGRKA
                     RVKKAK"
     gene            complement(12341..13027)
                     /gene="trmD"
                     /locus_tag="VFJ72_03370"
     CDS             complement(12341..13027)
                     /gene="trmD"
                     /locus_tag="VFJ72_03370"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:NF000648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET7478541.1"
                     /translation="MRSIDVFCVFPEVVDAALRVGVVGRAIERGEVSYRSFDLREYAP
                     GGRIDDMPFGGGAGMIVRVDVVARAFEEIYGVPARKVRESRRVIVTEAWGRTMSQGYV
                     AEVADGADVTVVCGRYGGVDERVRSELATESLSLGEFVLSGGEIVAAALADSVIRLRE
                     GVLGNAESLVGESYSGEGVIGAPQYTRPAVWDGEAVPGVLLSGNHAEIRAWRESRARE
                     RARTRVAERP"
     gene            complement(13031..13528)
                     /gene="rimM"
                     /locus_tag="VFJ72_03375"
     CDS             complement(13031..13528)
                     /gene="rimM"
                     /locus_tag="VFJ72_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7478542.1"
                     /translation="MNDPVVIGVIGTPHGVRGTVRVRATGSGRHLREGMEPRVGSMAY
                     EILSSRPTPKGFLLDLKGIGSRAEALALRGKEMVLDRSELDSPEDEEFYVADLVGLSA
                     HDSEERELGEVIGTFQTPAHEVLVIRPRGASDAARDFFVPFTLEHVPGLDPVSGRIVV
                     RPPEE"
     gene            complement(13540..13779)
                     /locus_tag="VFJ72_03380"
     CDS             complement(13540..13779)
                     /locus_tag="VFJ72_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET7478543.1"
                     /translation="MPDLENLVRILARSLVDEPEKVEVSSTESDSRVDLELKVADEDI
                     GKVIGRQGRTIRAIRTVVKAASVKAGKRTNVEVLD"
     gene            complement(13792..14034)
                     /gene="rpsP"
                     /locus_tag="VFJ72_03385"
     CDS             complement(13792..14034)
                     /gene="rpsP"
                     /locus_tag="VFJ72_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7478544.1"
                     /translation="MAVKIRLARHGSTKAPFYRIVVADARSPRDGKFIDRVGTYNPRT
                     QPSDIQVDVEKAKEWLDKGAQPTDQVRKLLKISGAL"
     gene            complement(14072..15403)
                     /gene="ffh"
                     /locus_tag="VFJ72_03390"
     CDS             complement(14072..15403)
                     /gene="ffh"
                     /locus_tag="VFJ72_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET7478545.1"
                     /translation="MFDTLGERLNKALDGVRGSGNLTEDQVKKVTREIRLALLEADVN
                     YGVVKEFVSNVRERATGAEVSKALSPGQQIVKIVNEELANLMGGTSHKLSFASRPPTV
                     VMLAGLNGHGKTTTAGKLALFSRKLGKNPYLVACDTYRPAAIEQLKQIGKELGVPVYD
                     EGADPKPEEIAERGVKKARDEGYDFVIVDTAGRQVVDMDLMEEIKRVRNNVRPHSVLL
                     VLDVVTGQNAVEVAQAFQEYAEYDGMVLTKLDGDARGGAALSVVSVTGKPIRFASEGE
                     KMSEFDYFYPDRMAGRILGMGDVLTLIEKAEQQVDRKEAEEQGKKLMSGKFTFEDFLQ
                     QMQMIKKMGPLSGLLSMIPGLNKQLKDVEIDDKQLGRVEAMITSMTVQERRNPKLLQN
                     PSRARRIARGSGSTQQDVQKLVKQFNEMQKMMRQMGKGGGMPRIPGLPGRR"
     gene            complement(15404..16573)
                     /gene="ftsY"
                     /locus_tag="VFJ72_03395"
     CDS             complement(15404..16573)
                     /gene="ftsY"
                     /locus_tag="VFJ72_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7478546.1"
                     /translation="MATETVQRSEEAPEAESGIEPEPEIIDDELRDAPEAEPIPEEQA
                     AEDAVSVELSELEAPEPEAPAPEPEEPEVGTEDLAGAAESEPESVGWFGRLRQGLTRS
                     RESFVGQLNAAVAEFRDAEDEEFWERVEEILISSDVGVPTTAKLVAELEQEALEKNIT
                     SGAELRRLMIEHAARMLEGPVELDISHHPTVILMVGVNGTGKTTTIGKLSRFLPGTTM
                     FAAGDTFRAAAIEQLQTWGERTGTPVIARERGADAAAVAHDAVEAAKRERTDVLLIDT
                     AGRLHTKVNLMAELDKVHRVIGRQLPGAPHEVLMTVDATTGQNGLRQAKMFQEVAGLT
                     GIILTKLDGTAKGGIALAIANEVGVPIKLISVGEKVEDLHPFDAREFAEAMFGDL"
     gene            complement(16649..>18214)
                     /locus_tag="VFJ72_03400"
     CDS             complement(16649..>18214)
                     /locus_tag="VFJ72_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478547.1"
                     /translation="AHDHPENGHVVVTRNGFRLTRTSVRLRSEDGRFSRKARLDREQN
                     RLRVLKEGPGGLLYDCREQLSQAGRQLEQIASHSQTTLSIVARTNRTSSALMREARSR
                     GIRLERARDALLEREREMEGIRDDLARAEASLSTAAEAAQEARRDVTASAAALEDAEA
                     AFSQLDRRRSRLRAAVAEGVRRRDGISKQLARTQAEPAGDADLPARLASRVAGVLRSL
                     APAVRERRSRLRRARSEADEEHRRLSAIRADLSRRAVDLARDLATARADAEHARQDLR
                     QAENASSEALEEIRSEWGATLEDARREGAEHGPETEAERHRLARKLKRFGDVNLLALA
                     QQDTLRERHEFVTAQRADAEAAANELNRIIHSIDGEIEQRFTETFGRVRRAFGEMVPR
                     MMDGAAGELDLSEEGVEIGLRLGRKGWRPLHVLSGGERALLALSFLFSIFLSRPGREG
                     GAFCILDEAEAALDDLNLARFIAVVDSYRSEGQFLLVTHQKRTMAAADVLYGVVQDAA
                     GATTVVSKRMQGE"
BASE COUNT         2956 a         6480 c         5494 g         3284 t
ORIGIN      
        1 ctggccttga gggagacctt gaagttaaag aagcccatct cctcggcgat ctcgaccttg
       61 tgcagcgcgc tcgccacgag agcttccgcc ttcggaaggt cccagtactt cttctccacg
      121 gagccggagt tgaccccgat cctcatggcg acgccggtct cctgacactt ctcgatgacc
      181 tgccggtagc ggtcgtcgct cccgacgttg ccggggttga tgcggacgca ggcggctccg
      241 gcgtcggccg cgccgagcgc catccggtag tcgaagtgga tgtcggcgat caggggtatc
      301 ggcgagcgcc acacgacctc gcggaagccc ttgagggcct tcgagccgtt gacggagaca
      361 cgaacgaggt ctgcgccggc ggcattcagg tcgtagacct gctggaccgt cgcctcgaca
      421 tcgtaggtca tggtgttcgt catggattgg acggcgatct gcgcgccgcc cccgatgggc
      481 acgccgccga ccgtgatctg ccggctcgtg atcggacgct cctgtatcgc tgcctctgtc
      541 atctcactcc tatatttgcg aacgctctta tcttagttcg ggatgaacga ttgtccggta
      601 aagagtttac taaggtccgc gtaagttgcg aacacgaaca gcaccaggac cagcgcgatc
      661 cccagcgccg caaccttccc catagtctcc tggctaaccg gacggcccag gatcttctcc
      721 atcacgataa agaacaggtg ccccccatca agcggcagga cagggagcag gttgaagatc
      781 gccaggttca ggctgatgac cgccagtagc tgcgggaaga acccctggct cacggccgtg
      841 cttccgacgg aagcgatccc gaccggaccc gtcacgttct tgtagaagtc ctgctgcccg
      901 gtgatgagtt gtccgataaa cacgaccatc ttgaccgtga tctcccagat cttctgcgcg
      961 gccatgcccg tcgcctccag cggcccgtag gttatctgcc gcacctccgg ccgcacaccc
     1021 acgagcgcct gccccggata ctgcttgctc tcgccgagct taccggagaa ctgcttctcc
     1081 gcgccgtcgc gtttcaccgt cactgagacc tcgtctcccg gcttcttgtc ccggacggcc
     1141 cgcgtgaaac cctcccactt ctgtatccgc tgtccgtcca cggccacgac ccgatcgccg
     1201 gcctggatgc cgacccggtc cgccatcgtg ccgcgctcga ccttgtccac gacaggcgtg
     1261 ccaacgacga ccccgctgat catgagcgtc ccggccagta tggcgattgc ggcgataata
     1321 ttggcgaatg gaccggcgaa gattatgagc gcccggcgcc acgctgcctt cgccggatag
     1381 gtgtccggcc cacgggcgtc gtcgttcatg ccggccatct tggcgaatcc accgagcagg
     1441 accacgcgga acgagtacac ggtcttgccg atcttctttt tgaacagcgc cggtccgaag
     1501 cctatgccga actccggcac ccgcacgccg agcgccttcg cggtcagcat gtgccccagc
     1561 tcgtggatga ctatgagcag gatcaggccg agcagcgcaa ctattaccgt catcgcgccc
     1621 gctccacgag cctcgcggcc tcgtcccgcg cccagcggtc cacgccctct atggcctcca
     1681 tgctcctcat ggtcccgaag tctggaacca actcaagtac ctcctcgatg agatcggcta
     1741 tctggaggaa ttgtatccga cgttcgagga acgcctcgac ggcgacctcg ttcgccgcgt
     1801 tgagcgccac gggataagcc tctcccgccc gcccggcctc gaccgcgagc ggcaggcagc
     1861 ggaacacgtc gttacgcggc tcctggaacg tccaggtcat ctctccgaag ccaaccggct
     1921 cggcgcccac gtccacccgc tccgggtaga gtatgccgaa ggctatggga agccgcatat
     1981 ccggcagcgc ggcgtgcatg atcacggacc cgtcccggaa gatagcgcca ccatgcacca
     2041 ccgactgccg gtgcaccacg acccgcacat cgtcgtagga agtcccgaac aggtgatggg
     2101 cctcgataac ctcaagcccc ttgttcatca tcgtggcgga gtctatcgtg atcttggccc
     2161 ccatgttcca cgtcgggtgc ttgagcgcgt cctccggcct cgcctccatg agacgtccct
     2221 tctcgaactc gtagaaaggt cccccggacg ccgtcaataa cacctccgcg acctccgctt
     2281 cgtcccgccc ctcaagacac tggaagatcg ccgagtgctc cgaatccact ggcagaatgg
     2341 gattgtccct ggcgaggtcc atgatccagc gtcccccggc cacgagggac tccttgttgg
     2401 cgagggcgac ctgatttccg gcctgcagag ccgccacggt cgcggagaga ccggcgaaac
     2461 cgacgatccc gttcagaacc acatcggcgg gcgctgcggc gagcgcgcga gccgatccgg
     2521 gaccgacaag cgtttcggcg gcgcagcccg agagcgcgtc cttctcgcct ttttcgagag
     2581 cgacgtactc gggattgaac tcggcggcct gggcgcgcaa gaggtcggcg ttcgaggcgg
     2641 cggagagacc gacgacctcg aagcggtccg ggtgggcgcg cacgacatcg agggcctgct
     2701 ggcctacgct gccggtagag ccgaggatag tcaggcggcg cgtcatattg gatggatggt
     2761 gttcaaagag ctcaaaacga agatgtagta taccgccggc ccggtaaaga gcaggctgtc
     2821 tatacggtcc aggataccgc cgtgacccgg cagggcgcgt cccaggtctt ttagttccag
     2881 gatacgcttg agtatggact cgaagaggtc cccgctctgg ctgaacacgg agataccggc
     2941 tcccatgagg gctgcgaacg ggagcgggag ccccaggaac acgttcgcga cccacccgac
     3001 ggcggcggtc gtgagcatca ggccgccgat gctcccttcg agggtcttgt tcggactcag
     3061 ggatgggaag agcttgtggc gtccgaaaag gcggccggcg aagtaggcgc cggagtccga
     3121 gatccagggt ccaaccactg ctatgaggat gagcgggata cggtcctgga gctgtgagat
     3181 caggccaagg tgcgccagag acgccccgac ccacagcgcc atgagaagga tggcgagaac
     3241 cgcccgcagg gtccgtgcct ccgggcgccc ggcgagccag aacaatgccc agggcaggct
     3301 caacagggca ccggccagga tgccggtctc ggcgtaaggt atggagagaa gtatcgggat
     3361 gacgcccgcg ccaagcgcgg ccgggaacgg cagcgggttg agagcccgcg acaactcgta
     3421 ggcggagagt cccaccacca gcaggaccag cagcagcacg gcccaggtcc cgagcagtat
     3481 cgcgccgaga gctattggcg ccagtaccag agcggttacg gtgcgccacc ggagcacggg
     3541 cgctccccta aacgccgccc cggcgccggg accgggaggc gtaagattcg atggcgtgga
     3601 agaactcctc gcgcgagaag tccggccaga gcgtgtctgt gatgtagaac tccgcgtagg
     3661 cgatctgcca gaggaggaag ttcgagaccc gcagctcgcc gctcgtgcgg atcaggaggt
     3721 ccacctccgg gacttcgggg gcgtagaggt agctggcgaa ggtcgcctca tccacctcct
     3781 ccggggtgag accgtcggcg acgatgcgcc gggcggcgtc cactatctcc gagcgcccgc
     3841 cgtagttgag cgcgatgtag acctccaggc ggtcattgtg cgcggtaagg tcttcggcct
     3901 ccagcatcgc ggcctgtacc gactccggca aatgctccct gcggcccagg aagcgcagtc
     3961 gcgcgccgcg ctcgttgagc tccggcacct tgcgccgggc ggtttcgagg aagagggaca
     4021 tcagggtgtc cacctcgtcc accgggcgcg cccagttctc ggtcgagaag gcgtagaggg
     4081 tcagggtctt aacgccggcc tcgccggccg cttcaaggag cggggtgaga acggagacgc
     4141 ccgcccggtg tccggcggcg cgcgggagcc agcggcgctg agcccatcgt ccgttgccgt
     4201 ccatgataat ggcgacgttg cggggcacgc cagcggggtc gaggcgagag aggggtctcg
     4261 cttcggcagg ggtcttcggc gggtgtttac gggcgggttc cagggtctag acctccagga
     4321 gctcggactc tttatccgag agagcggcgt ctatacgctt gacgtaatcg ttggtcagct
     4381 tctggagctc ctgctcggcg cggcgctcgt cgtcctcgga gatctcgccc atcttcttca
     4441 actcgacgat gtcgcgcatc tcgtcgcggc ggacgttgcg gacggagacg cggccctctt
     4501 cggccatgtg gcgggcgacc ttgatcaact ccctgcgccg ctcctcggtc aactccggga
     4561 tcgggaggcg caggatcttg ccgtcgttct ggggattgag gcccacgtcg gagtgctcga
     4621 tggcccgctc tattgccttc agagagttag gatcgaacgg cgtaacggtc atcaggcgcg
     4681 gctcgggcac gttgatgttc gcgacgctcc gtagcgccat cttcgagccg tatacctcca
     4741 cctcgacccg gtccagaata gccgggttcg cgcgcccggt acgtatggtg gcgaagtgcc
     4801 cccgcaccga ttcgagcgcg ccgtgcatcc ggcgttcggc cgccgtgaga tctatcatgt
     4861 cggacatctt ctactcccct gtagccccgt cgctggtcgg gccgccgtca gccgcgcggc
     4921 cgcgcgcgct ccaaaccagc gaacccacgt gttcaccctg cagaatgcgc cgcaggtttc
     4981 cgggcttgag tatatcgaat accactatgg ggaggttctc tccgctgcac aaagtagccg
     5041 ccgtgtggtc catcacggcc aggttctgag agagcaggtc catatagttg aggcgcggca
     5101 cgcggacggc gtccgggtga gtgcgcgggt ccttgtcgta gatcccgtct acctggttct
     5161 tcgccatgag cagggcgtct gccccgatct ccagcgcccg caaggccgct ccggtatccg
     5221 tggagaagaa cgggttgccc gtaccggcgg cgaagataac gacccgcccc ttctccaggt
     5281 gacggatcgc gcggcgccgg atgtacggtt cggctacctc ctggatctgg accgccgact
     5341 gcacccgcgt ggggacgccc cggccttcga gagcggcctg cagcgccatc gcgttcatga
     5401 cggtgccgag catgcccatg tagtccgccg tcgcgctctc tatgccaagc tcgtcagcca
     5461 ccgaagagcc ccggaagata ttgccgcccc cgaccaccat cgccagttcc gccccgccct
     5521 cgaccgcttc gaggacctgg ctaacggtgg actgtaggct cacggggtct atcccatacc
     5581 cctcctcccc cgccaggctc tcgccggaga gcttcagcac gacgcgcttt accggtccca
     5641 gctccgcgtt gggtgcctcg tcgccgccct tgaaagccgc ctggctcacg gctcgctaga
     5701 gctcgtagcg gacgaaccgc cggatcacga cgttctcgcc gaccttctgg accgtgtcct
     5761 ggagcaagtc cccgaccgtc ttctccggtt ccttgacgaa cggctgggtc aggagcgcct
     5821 gctcggcgac ccacttcttg atcctgccgt ccacgatctg gcgcgtgatc ttctcgggct
     5881 tgcccatctc cgccgcctgc ttctcggcga ttgccctctc ctcgtccatg gcgtcctgcg
     5941 ggatgtcctc cggcgcgaca cacagcgggt tcatcgaggc gacgtgcaac gcgacgttcc
     6001 gcgcgaactc cttgaactcc cccgtccggg ccacgaagtc cgtctcgcag tccacctcga
     6061 ccattacgcc gacgcggccg ttgaagtgga tgtacgcctc tatgagaccc tcgttggtcg
     6121 cccggccgct cttcttctga gcctgcgcct gaccgcgctc ccgaaggagc ttgcgcgcac
     6181 cctcgacgtc gccattcgcc tcggttacgg cgcgcttcac gtccatcatc ccggctccgg
     6241 tctcctcccg gagctgcttt atcttctcta tcgtgctagc caaagtctct acttctcctc
     6301 ggtctcatcg ccctcagccg gcgcctcggt tacggcttcg gcctcttcct cgttcgccgc
     6361 ttcgggaccg gccacctccg ggtccgctat ctctgggtcc gtcgcctccg gcgccgccag
     6421 cgaagccccc ggctgcggag cttccggctg ctggggctcc ggctcggcac ccgttccgtt
     6481 ggcgctcgca ttagcgctcg cggaggactc ccgggccgct tcttcggaga cgagggcctc
     6541 ctcaacgacc ggagggaccg ggcgatcctc ggccgggact tcagtctcgc ccttaccagc
     6601 gagaacggcg tccgcgacca ggcgggagat caggttaatc gcccggatcg cgtcgtcgtt
     6661 tccggggatc acgtagtcta tgacctccgg gtcgcagttc gtatcggtga gcgcgacgac
     6721 cggtatcctc agccggttgg cctccttgat caggagctcc tcgcgcttgg ggtccacgac
     6781 gaacaccgct ccgggcagcc gctccatctc cgtgatgccg gcgaagttgc ggcgcagctt
     6841 ctggtactcg cggttcaggg cgaaccactc gcgtcccgtg cggttctcct ccggcgtctc
     6901 ctccacccta gccgcgaggt tcacgaagtg ctggatgcgc ggctggatcg tctggaagtt
     6961 cgtaagcatc ccgcctatgt accgctccgc tacatagggc tgtccgctgc gcatcgcctg
     7021 ctcggcgatg gtcaactggg cctgcttctt cgtgccgacg aacaggacct cgcgtccggc
     7081 ctccgcgatg ctgcggatga acttcagcgc atcctcaagg agggtgatgg tctggtccag
     7141 gtctatgatg tgaacgccgg cccgctgggc gaagatatag cgcttcatct tcgggttcca
     7201 gcgcttggcc tgatgcccga agtggacccc ggcttcgagc agctctctga cacctacacg
     7261 ttcggctgtg ctctccatga ttctctcctt ttcaggtggt tgatagcctc cgcccgcttc
     7321 tcgcaatcca cggtgagctt ccgcggacca ccacctgggt aatcgaccct gcgcgggcgt
     7381 gcgtgattaa aacctcgaag atgttagcac acgggatacg agccttctag ccgagcggga
     7441 aggcggtccc gtccgagagg tccgcgcgca gtagctccag agcgcggcgc agtatctgcg
     7501 agatgcgtcc ttccgtcagg ttcatcgcca tgccgatctc cttgagggtg agtccctcgt
     7561 agaagtaaaa ggtcgcaacc acgcgctccc gctccgccag gcgctccagc gccgcgacga
     7621 gcctgccccg gacctccgtc ttctccgcct cggactgcgg gtcagacgcc gagctgtcag
     7681 ccacgaagtc cccgtactcg gacccggcgc ggttatccaa ctccatcctc gcttcgagcg
     7741 acccgaccat agcctgcgaa tatcgatcca acatgtcccg gtgtctctct acgctgacgc
     7801 cgagcgctcg cgccagctcc tcctccgtcg gcggccgtct caactcctgc gtgacccgta
     7861 ccctcgcggc ctcaacctcc cgaacgtgcg cgcgcacccg ccggggtaca cggtcctctc
     7921 tcctgagctc gtcgaggatg gaccaccgga tcttggatat cgcataagac tcaaactttg
     7981 cgccgcgatc cggctcataa gtctggatag cgtccaggag acccatgatg ccccaggaca
     8041 tgaagtcctc ctgatcgaac gcccccgtca tgcgcccccc gagcctgccg gccacgtact
     8101 tgaccagcgg tgagtagttc acaagcaacc tgtcgcgcaa ctggttggcc tcgcggtaca
     8161 gatcgtcccg cttaccagac tcggcccgat ccgcttcctc cagatggccg cgtacttcta
     8221 ggtatcttgc ccacagacgc ccgagggtct gtctcacacc gtgctcctgc gcacgaacgc
     8281 gccgctgacg ttacgcccca cgtagccttt tagctccagc atggtcagcg cgggcaacag
     8341 gtcgcgcatc ccgatgccgc tgcgggagga tattacgtcc acctccgtcg gctcgaaacc
     8401 cacgccggca agtacggccg cctcgcgctc gggcaacccg gcggggaggt cttcggacct
     8461 cgcctctccc cgttgtggaa gcgtctcctg aaccggagcg accgcgtcca cgaactcccg
     8521 gatgtcccag agcacccccg ctccctcgga gagaagtttg ttcgatcccc ggcactcggc
     8581 caccccgaca ggccccggca ccgcccagac gtcgcggccg gaatcaagcg cgtggcgggc
     8641 cgtgatcagg gccccgctct tctccgccgc ctcgacgacc acgagggtct ccgcgagtcc
     8701 cgcgatgatc ctgttccgcg ccgggaaacg ccacgcgagc ggagcctcgc cgaggtaata
     8761 ctcggataga acggctccgg ttgtgctgac acgcgcgtag aggtcgcggt tgcgtctcgg
     8821 gtagatcacg tctatgccgc aaccgagcac gcccacggtc ggcccgtcgg ccatcaatgt
     8881 cccctcgtgg gccgccgcat ctatcccgag cgcgagaccg ctcaccacgc agacgccgcg
     8941 cccacccagc gcccgcccca gctcccgcgc aacctcgatg ccgctgacgg aacacttgcg
     9001 cgagccaacg accgcgaccg ccggacccgc cggtaacgca ccgttcacga acagagcggg
     9061 cggagcgtcc ggcacctcgc gcagccgctc agggtaatct tcatcggcga gcgtaacagc
     9121 ccggattccc ttctcgctca gacgccgctc gacggtctcc gcgtcgaagg tgcgtttcag
     9181 atcatcgaac gcctggcggg cgcgctcggt cagcttgatc ctcgcggcaa gctcgcccga
     9241 agacatttcg ggtacggcct cgggcgcgag atccccgaga cgcgagatca cgctggtccc
     9301 cacccgcgcc tggaggagcg agaggaacag gtaggccgcg cgccctctag cggtcaatta
     9361 ggctggatct cctgtagttc agagcttcgg cgaggtgttc gacctgaata tcccgctcgc
     9421 cggagaggtc ggcgaccgtc cgggccacgc gcagcacccg gtcgtgcccc ctgccggaga
     9481 gtcccaggcg atctatcgcc cgcgacagga gcgcctccgc gtcaccgccg accgcgcaca
     9541 cctcgcgcag ccgcctcccg gagagcgccg agttcggtcc tccctggcgt tccgtctgta
     9601 tccggcgcgc cgcagcgacc cgctcccgta tcctccgcga agactccgcg ggatggctct
     9661 cccgcaactc ctcccgcgtc aggcgcggca cgtctacgaa gaggtccagc cggtccagca
     9721 aaggaccgga gatgcggctc tggtagcgtt cgatctggcc cgccgagcac ctgcacgctc
     9781 gccgccggtc tccagcgtac ccgcaagggc aggggttcat ggagcacacg agcgtgaccc
     9841 gcgccgggta gctcagggtc gcggcgaccc gcgagatcgt cacgtgtccg tcctccagcg
     9901 gttgacgcag cacctccagc gtaccgcggc tgaactccgg gaactcatcc aggaacaaca
     9961 cgccgtgatg ggccagcgag acctcgcccg gacgcgggtt gctccccccg cccgcgaggc
    10021 cggaggtgga gatagtgtgg tgcggcgccc ggaaaggccg ggactttatg agcgaccccc
    10081 ggctctcccc cgccgcgctg tgtactttcg tgacctcgat gctctcccgc gtcgtaagcg
    10141 gcggcagtat ccccggcagt ctccgcgcca gcatggtctt ccccgaaccc ggcgggccgc
    10201 tcatcaggac gttgtggccg ccagccgcgg cgacctccag cgcgcgcttg gcgtagccct
    10261 gtccagcaac gtccgcgaag tcgttgtcct gaaggtccac ctccaccgtg ccgttctcgc
    10321 tcgcggtcgc aggctccatc cgcgcccgac cgccgaggaa gtccaccgcc tcgcgcagcg
    10381 tcctcatccc gtagacgccc acctcaccga tggcggccgc ctctgctgcg ttcggttccg
    10441 gcaggatcag gcgcggcatc ccttcccgcc gggcgccttc ggccatcgag agggctccgc
    10501 ggatactccg cagtccaccg tcgagcgaga gctctcccac gagtccagct ccgccgagac
    10561 gctccgccgg catcgctccg gaggcggcga ggaccgccag agctatcggg aggtcgaaag
    10621 caggtccctc cttacgaaga tgcgcgggag cgaggttgac gatcaccttc ttcgtcggga
    10681 acttgtagcc gctgttagag atcgcgacct tcacccgttc gcgcgcctcc tgcaccgccg
    10741 tatcgggcag gccgacgata gagaagcctg gaagacccgc gctcacgtcc acttcgacct
    10801 cgacaggcag agcgtctatg cctgccagcg taagactccg aaccctgcac accgacacta
    10861 agcgtctact cctcccctgg atgcgtgccg cgatgctcct ccacgattca gcgacaccgt
    10921 ccttcgcgca ttaccagtcc tataataact catgtaccgt cggaacccga gactccaaac
    10981 aggtcgccgc ttagaaagcg ttttcgaggt gctgaatctc cggcggacca tgccctgaag
    11041 cgaggatacc gaccacgtcg aaccgcaact cgtcgaactc cgggtcgcgc tcagccaggt
    11101 actgctcggc catcgaccgg accgcggcct gcttgcgggg agtcacggcc tccgttggat
    11161 ggccgaactc ccgcccgcgc cgcagcttca cctccacgaa cacgagggtg tcaccatcac
    11221 ggacgacaag gtccaactct ccataccgcg tccggtagtt gcgttccacc agcgtatagc
    11281 ccttcctcac caggtaacgg agagcgaggt cctctcccca gtcgcctgcg ctgcgggttc
    11341 tcagggaccg accccggcgt agctcatcct gtggacccgg cacggcccaa cctccgcgag
    11401 agcctgtctg tgctccaagg taccatagcc gcggttgcgc tcgaagccgt accggccata
    11461 ctcgtccgcg agcgcgcgca tggcatggtc gcgcagcacc ttcgcgacga tgctcgccgc
    11521 ggcgaccgtc gcgctagcag cgtccgcacg gggcaaacac tcgatctctg gtcccagacg
    11581 caggttccca tccgcgaggc cacaaccagc atgagagtcg aggagggcga tggcccgccg
    11641 taaagcctcc ctgttggcga ggccgagccc gtgaagatca atccaccacg ccggaaacgc
    11701 gaccacggaa accgctctcg cgcaccggag cacctcgccc agtagacgct cccggacggg
    11761 cgcggagagg agcttggagt cccggacctc cagaacctca tcctcgccga ggatcgcggc
    11821 ggcagccacg atgggtccgg ccagcgcccc gcgtccggcc tcgtccgctc cagcgagcgg
    11881 ccctctacgt tcgaggagcc acgccgcatc aaatgcgtag agggagccgc ccgatggacg
    11941 accccctcca ttccgtcccg ccaacctggc gagaagctac ttggccttct taacgcgagc
    12001 cttgcggccg atcttgtccc tgatgtagta cagcttcgcg cgccgcaccg cgccgcgcga
    12061 ggcgacctcc aacttggcga tcttcggcga gtgtaggggg aagatcctct ccaccgccac
    12121 tccgaaggag ttcttgcgca cgacgaacgt ctctccgatg ccgctcccct tacgggcgat
    12181 acacagaccc tcgaagatct gggtccgttc ccggttgccc tcgatgaccc ggtaatgcac
    12241 ccgtacggtg tcgcccggcc ggaaggtgac gtccttctgc tgcaggttct cgctcgttat
    12301 catattccgc tcctctctcg ggaagtgata gagaacaggg ctacggccgc tccgcaaccc
    12361 tcgtccgcgc tctctctcgc gcccggctct cgcgccaggc gcggatctcc gcatgattgc
    12421 cggataatag cacgcccggc accgcctctc catcccacac cgcgggcctc gtatactgcg
    12481 gcgccccaat aactccctcc ccggagtaag actcgccgac gagactctcc gcgttgccca
    12541 gcacaccctc cctgagccgt atcaccgaat cagcgagcgc cgccgcaacg atctccccgc
    12601 ccgagagcac gaactctccg agtgagaggc tttcggttgc aagctcgctc cgcacccgct
    12661 catcaacccc gccgtagcga ccgcacacca ccgtcacgtc cgctccatca gcgacctccg
    12721 cgacgtagcc ctgggacatg gtgcgacccc atgcctcggt cactatgacc cggcgtgact
    12781 cgcggacctt gcgcgccggg acgccgtaga tctcctcgaa tgcccgcgcc accacgtcta
    12841 cccgcacgat cattccggcc ccgcccccga acggcatgtc gtctatccgc ccgccgggcg
    12901 cgtactccct caggtcgaag ctgcggtagc tcacctcgcc ccgctctatc gcccggccca
    12961 caaccccgac ccgcaacgcg gcgtccacca cctccgggaa gacacagaag acatcaatgc
    13021 tacgcaagga ctactcctct ggcgggcgga cgacgatacg tcctgatacc ggatccaacc
    13081 cgggaacgtg ctccagggtg aagggaacga agaagtcgcg tgccgcatcg ctggcgccgc
    13141 gaggccggat cacgagcacc tcgtgcgctg gcgtctggaa cgtcccgatc acctctccga
    13201 gttcgcgctc ctctgaatca tgcgccgaga gccctaccag atccgcgacg tagaactcct
    13261 catcctccgg cgaatcgagc tcggacctgt caagcaccat ctccttgccg cgcaatgcca
    13321 acgcctcagc tcggctgcca atgcctttga gatccagcag aaagcctttc ggtgtggggc
    13381 gcgaagagag tatttcgtaa gccatacttc ctacccgcgg ctccatgccc tcgcgcagat
    13441 gccgcccgga gccggtagct cgcacccgga cagtaccgcg gacgccgtgc ggcgttccga
    13501 taaccccgat tacaaccggg tcgttcaccc gcacagcgcc tagtccagga cctccacgtt
    13561 ggtacgcttg ccggccttca ccgaggccgc cttaacgacc gtgcggatcg cccggatcgt
    13621 gcgtccctgc cgcccgatga ccttcccgat gtcctcgtcg gccaccttca actccaggtc
    13681 cacgcgcgag tccgactccg tcgaggagac ctccaccttc tccggctcgt ccaccaggga
    13741 gcgggcgagg atcctgacca ggttctccag gtccggcata cgctctacga cctacagagc
    13801 gccggagatc ttgagcagct tgcggacctg atccgtgggc tgggcgccct tgtcgagcca
    13861 ctctttcgcc ttctcgacgt ctacctggat atccgagggt tgggtgcgcg ggttgtacgt
    13921 cccgacccga tctatgaact tgccgtcgcg cgggctcctc gcgtccgcta caacaatccg
    13981 gtagaacggc gccttcgtgg aaccgtgcct cgccagcctt atctttaccg ccatcttctc
    14041 accttccgaa ctctcactct ttatctactg tctatctacg tcctggcagt ccggggatgc
    14101 gcggcattcc gccgccctta cccatctgcc gcatcatctt ctgcatctcg ttgaactgct
    14161 tgacgagctt ctgcacgtcc tgctgagtcg agccgctgcc gcgcgcaatc ctacgcgccc
    14221 ggctcgggtt ctgcaacaac ttggggttgc ggcgctcctg gacggtcatg gatgttatca
    14281 tggcttcgac ccggccgagc tgtttatcgt ctatctcgac gtcctttagc tgcttgttca
    14341 ggccgggtat catgcttaga agacccgaga gcggacccat cttcttgatc atctgcatct
    14401 gctgcaggaa gtcctcgaac gtgaacttgc cgctcatgag cttcttgccc tgctcctcgg
    14461 cctccttgcg gtccacctgc tgctctgcct tctcgatgag cgtgagcacg tcacccatcc
    14521 cgaggatgcg cccggccatc cggtccgggt agaagtagtc gaactcgctc atcttctcgc
    14581 cctcggaggc gaaccggatc ggcttccccg tcaccgacac gacggagagc gccgccccgc
    14641 cccgcgcgtc gccgtcgagc ttggtgagca ccatcccgtc gtactccgcg tactcctgga
    14701 acgcctgcgc aacctccacc gcgttctggc ccgtgaccac gtcgaggacg agcagtacgg
    14761 agtgcggccg gacgttattg cgaacccgct ttatctcctc catcaggtcc atgtccacga
    14821 cctgccggcc tgctgtgtcc acgatgacga aatcgtagcc ctcgtcgcgg gccttcttta
    14881 cgccgcgctc ggcgatctcc tcgggcttcg ggtccgcgcc ttcatcgtag accggcacgc
    14941 cgagctcctt gccgatctgc tttagctgct cgatggccgc cggacgatac gtatcgcagg
    15001 cgacgagata cgggttcttg ccgagcttgc gggagaacag cgcgagcttt ccagcggtcg
    15061 tcgtcttacc gtgaccgttg agacccgcga gcatcacgac cgtgggcggg cgggaggcga
    15121 acgagagctt atgggaggtg ccgcccatga ggttcgccag ctcttcgttg acgatcttga
    15181 cgatctgctg gccgggcgag agcgccttcg agacctcggc gcccgtggct cgctcccgga
    15241 cgttcgagac gaactccttg acgacgccgt agttcacgtc ggcttcgagg agggccagac
    15301 ggatctcgcg cgtaaccttc tttacctggt cctcggtgag gttcccgctg ccgcggaccc
    15361 catcgagcgc cttattgagc ctttcgccta aggtatcgaa catctagagg tctccaaaca
    15421 ttgcttcggc gaactcgcgg gcgtcgaacg ggtgcaggtc ctccaccttc tctccaacgg
    15481 agatgagctt gatcggcacc ccgacctcat tggcgatggc gagcgcgatg ccgcccttcg
    15541 ccgtcccgtc gagcttggtc agtatgatcc ccgtgagccc cgccacttcc tggaacatct
    15601 ttgcctgccg cagcccgttc tggccggtgg tcgcgtcgac cgtcatcagg acctcatgcg
    15661 gcgcccccgg taactgccgc ccgatgacgc ggtgtacctt gtccagctcg gccatgaggt
    15721 tgaccttggt gtgcaggcgg cctgcggtgt ctatgagcag cacgtccgtc cgctctcgct
    15781 tggctgcttc gaccgcgtcg tgggcgacgg cggcggcgtc cgcgccgcgc tcccgtgcga
    15841 tcacgggcgt cccggtccgc tcgccccagg tctggagctg ctcgatggcc gccgcccgga
    15901 aggtatctcc ggccgcgaac atcgtggtgc cgggcaggaa gcgcgagagc ttgccgatgg
    15961 tcgtcgtctt gccggtgccg ttgacgccga ccatgaggat gaccgtcgga tggtgggaga
    16021 tgtcaagctc gaccggccct tcaagcatcc gggcggcgtg ctcgatcatg agccgccgca
    16081 actcggcgcc gctggtgatg ttcttctcca gcgcctcctg ctccagctcc gcgacgagct
    16141 tcgccgtggt tggaactcca acgtccgatg agatcaggat ctcctctacc ctctcccaga
    16201 actcctcgtc ctcagcgtcc cggaactccg cgacggcagc gtttaactga ccgacgaaag
    16261 actcccggct ccgggtcaat ccctgccgca gcctcccgaa ccacccgaca gactccggct
    16321 cggattccgc cgcgccggca agatcctccg tccccacctc cggctcctcg ggttcggggg
    16381 caggcgcctc tggttcgggc gcctctaact cgctcaactc gacgctgacc gcatcctctg
    16441 ctgcctgctc ttcggggatc ggttccgcct caggagcgtc ccgaagctcg tcatctatta
    16501 tctccggttc cggttcgatc ccggattcgg cctccggtgc ttcttcggaa cgttgcaccg
    16561 tctcggtagc cacagacgag ccggactcct gctcctcgtc tctggttctt ctaaagaaat
    16621 tgcgccacga acgagccatt ggcctgctct actctccctg catacgtttc gatacgactg
    16681 tggtggcacc ggcggcatcc tggacgacgc catagagaac gtccgccgcg gccatggtgc
    16741 gcttctgatg ggtgaccagc agaaactggc cttccgaccg gtaagagtct accacagcga
    16801 taaaacgggc caggttgagg tcgtcgagcg ccgcttcagc ctcgtcgagt atgcagaacg
    16861 ctcccccctc gcgccccgga cggctgagga atatgctgaa gaggaacgag agcgccagga
    16921 gcgcccgctc cccgccggaa agtacatgta gcggccgcca tcccttccgg ccgagccgca
    16981 gaccaatctc gactccctct tccgagagat ccaactctcc agccgcccca tccatcatcc
    17041 gcggcaccat ctccccgaac gcccgccgca ccctcccaaa cgtctccgtg aaacgctgct
    17101 ctatctcccc atctatcgag tgtattatgc ggttaagttc gttggcggcg gcttcggcgt
    17161 cggcgcgttg ggcggtgacg aactcgtggc gttcgcgcag ggtgtcctgc tgggcgagcg
    17221 ctagcaggtt gacgtccccg aagcgtttga gcttgcgggc gagacgatgg cgttcggctt
    17281 cggtctcggg accgtgctcg gcgccctcgc ggcgggcatc ttcgagggtg gcgccccact
    17341 cggaccgtat ctcttcaaga gcttcggagg aggcgttctc ggcctgccgg aggtcctggc
    17401 gcgcgtgttc ggcgtcggcg cgggcggtgg cgaggtcgcg ggcgaggtcc actgcccggc
    17461 gggagaggtc cgcgcggatt gccgagagac gccggtgttc ctcgtccgcc tcggagcgcg
    17521 cgcggcgcag gcggctccgg cgctcacgaa cggccggcgc gagcgaccgg aggactccgg
    17581 ccaccctgga ggccaaacgc gcgggaaggt ccgcatctcc ggccggctct gcctgggtcc
    17641 gcgcgagctg cttcgagatg ccgtcccgcc gccggacacc ctcggcgacg gcggcacgca
    17701 accggctcct gcggcggtcc aactgtgaga acgcagcctc ggcgtcctcc aaagctgcgg
    17761 cggacgcggt tacgtcccgg cgcgcctctt gcgccgcctc ggcggcggtg gacagcgatg
    17821 cctccgcccg cgcgaggtcg tctcggatac cctccatctc gcgttcgcgt tcaagaagag
    17881 catccctcgc tcgctccagg cggatgccgc gggagcgcgc ctcgcgcatc aaggcggagg
    17941 aggtacggtt tgtccgggcg acaatggaga gagtcgtctg ggagtgagat gcgatctgct
    18001 ctagctgccg gcctgcctgc gatagctgtt cgcggcaatc gtagaggagt ccacccggtc
    18061 cctccttcag cacccgcaag cgattttgct cccggtccag ccgcgccttc cgggagaagc
    18121 gtccgtcctc agaacgcagg cgcacgctcg ttcgggtgag acggaagccg ttgcgggtga
    18181 ccactacatg accgttctcc gggtggtcat gcgc
//
LOCUS       DASSMB010000048         4588 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1278734, whole genome shotgun sequence.
ACCESSION   DASSMB010000048 DASSMB010000000
VERSION     DASSMB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278734"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFJ72_03405"
     CDS             <1..507
                     /locus_tag="VFJ72_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7478548.1"
                     /translation="PVALVLTRQALPTLDRSKYAPASGVEKGAYVLADAEDGKPDVLL
                     LGTGSEVQLCIEAYEQLKGQGVKARVVSMPSWELFEQQSQEYRESVIPPDVTARVSVE
                     QATTLGWRKYVGLDGRTIGMRTFGSSAPLKELQEKFGFTHQAVAGEAMDLLGRSGNGS
                     ASPGGGRS"
     gene            702..1490
                     /locus_tag="VFJ72_03410"
     CDS             702..1490
                     /locus_tag="VFJ72_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7478549.1"
                     /translation="MATGFKGAIFDVDGVLVDSPHEMAWREGLKKLMENEWSDIRDQT
                     SYSPEKFTPQVYQREMSGKPRYKGAQAALEYFNVPDIERRAQEYGDMKQEMIVELIEA
                     KDFEEYPDALRFVLALKEAGVLIAAASSSKNAGAFLRLVRLDEFAESEGLSYDFLTPG
                     YTLLDILDGDVSGRDFEQGKPHPMIFLAAAEELGLSPEDCFVVEDAANGIQAAKAGEF
                     AALGIARADDEEALAEAGADIVVTSLDDVSLDAISEGRLEKAGS"
     gene            1490..3178
                     /locus_tag="VFJ72_03415"
     CDS             1490..3178
                     /locus_tag="VFJ72_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478550.1"
                     /translation="MSYKDNASLGGYEDTISGALSELRDERIVERMRDGDHTVWGPEP
                     DEISNRLGWLKSPETSEEALPEILRFAEAVKGEGIEHVLLLGMGGSSLAPEVFGFALE
                     RKEGYPDLAVLDSTDPEAVLRHAGSLDLSRTLFVVSTKSGGTVETFSFFKFFYNKVAE
                     IVGAERASSHFVAITDPGSGLQDTAEKYGFRQTFLNDPNIGGRYSALSFFGVVPAALA
                     GVEVAKLLNSGKDAAREFAGTEGEDNPGAWLGAVMGELARSAGRDKLTLLASPALAPF
                     GPWVEQLIAESTGKDGVGILPVAEEPEGSPEVYGDDRFFVSMRLRSEEPDALLDELKS
                     QGHPTVEVLLDDPYDLGGEMFRWEVATAISGWRLGINPFDQPNVESAKIQARKMVAEY
                     QEKGELPEPEPTVEDGGISVYSSGGAASVEEALEGFLSQARPGDYVSLQAYLPPSDET
                     TETLQGLRVRLRERLKLATTLGYGPRFLHSTGQLHKGDGGNGLFIQITSDPREDAPIP
                     DEAGKPEATLTFGVLEAAQALGDRRALLDVDRRVIRFHLGGDVSAGLDRLANSI"
     gene            3320..>4588
                     /locus_tag="VFJ72_03420"
     CDS             3320..>4588
                     /locus_tag="VFJ72_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET7478551.1"
                     /translation="MAEGPYLMGIDFGTGGVRVGVFDREGTPVVFHGVEFDTKFPRSG
                     WAEQDPDVWWSSFVEATKKVMQEGGISPEEIAGISTDSTASTVLAMDANDKHLRPAIM
                     WMDVRASDQAKKLEDTHDNALKYCGYGAVSAEWGLPKALWLKENEPETYENARHICDC
                     NDWLINRLTGEWKSSVNIASAKFFYDRDEGGWPETLLEAVGFEDFLDKFPPDVLDLGE
                     VVGGLRKDVADELGLKEGTPVAQGAIDAYAGALGLGVVEPGTLALITGSSHVMIGQAA
                     RPTHGQGFWGSYTDAMIPGQYTVEAGQVSTGSVVAWFKNNFASEAAKEAESRGVDTYD
                     VLNEIAEKVPLGSNGLIFIDYFQGNRSPHTDPLVRGAIWGLSLGHTPGHIFRSIIEGI
                     CFGTESIFRAMRGHQFEPRLNVVSGGPAKSE"
BASE COUNT          863 a         1410 c         1595 g          720 t
ORIGIN      
        1 cccgtggccc tggtcctcac ccgccaggcg ctgccgaccc tggaccgctc taaatacgcc
       61 ccggcatcgg gtgtcgagaa gggagcctac gtactggctg atgcggagga cggcaagccc
      121 gacgtcctgc tgctggggac cggcagcgaa gtgcagctct gcatagaggc ctacgagcaa
      181 ctcaaagggc agggggtgaa ggcccgcgtg gtgagcatgc cctcgtggga gctcttcgag
      241 cagcagagcc aggagtaccg tgagagcgtc atccctccag atgtaaccgc gcgcgtctcc
      301 gtggagcagg ccaccacgct cggttggagg aagtacgtcg ggctggacgg gcggaccatc
      361 gggatgagga ccttcgggtc ttccgcgccg ctgaaagagt tacaggagaa gttcggcttc
      421 acccatcagg ccgtggcggg agaggcgatg gatctgctgg gcaggtccgg gaacgggagt
      481 gcttcgcccg gtggaggtcg ctcctgagtt cgtatgggag ctttcaggta cgggtagagg
      541 tatcggaagc atctggagtc gaccacgaag acataacgga ggtgctagag gagagcggcg
      601 aaagttcggc gactcctata atgaagtgat ggaacagata agggccatag gaaatcatat
      661 ggcccaccag tcctgaaagg tattccgggg agaggaggag tatggcaacg ggtttcaagg
      721 gcgctatctt cgatgtggac ggggtgttgg tggattctcc gcacgagatg gcctggcgcg
      781 agggcttgaa gaagctcatg gagaacgagt ggagcgacat aagagatcag acctcgtatt
      841 cgccagagaa gttcacgccg caggtctacc agcgggagat gagcggcaag ccgcgctaca
      901 agggcgccca ggccgccctg gagtacttta acgtgcccga catcgagcgt cgcgcccagg
      961 agtacggcga catgaagcag gagatgatag tagagctgat cgaggcgaag gacttcgagg
     1021 agtacccgga cgccctacgc ttcgtcctgg cgctcaagga ggccggcgtc cttatagcgg
     1081 cggcctcatc atccaagaac gcgggcgctt tcctgaggct cgtgcgcctg gacgagttcg
     1141 ccgagagtga gggtctctcc tacgatttcc tgacgccggg atatacgttg ctggacattt
     1201 tggacggcga cgtgagcggg cgggacttcg agcagggcaa gccgcacccc atgatcttcc
     1261 tggccgccgc cgaggagttg ggactttcgc cggaggattg cttcgtggtc gaggacgcgg
     1321 caaacggcat acaggcggcc aaggcgggag agttcgccgc cctgggtatc gcgcgggcgg
     1381 acgacgagga agccctggcc gaggccggcg cggacatcgt cgtgaccagc ctcgacgacg
     1441 tctcgctcga cgcgatctcg gagggccgtc tggagaaggc gggctcctga tgagctacaa
     1501 ggacaacgcc tccctgggcg ggtacgagga tacgatttcc ggcgctctat cggagttgcg
     1561 ggacgagcgg atcgtggagc gcatgcggga cggcgaccat actgtatggg ggccggagcc
     1621 ggacgagatc tccaaccgcc tcgggtggct gaagagcccg gagacctctg aggaggcgct
     1681 gccggagata ctccgcttcg ccgaggcggt gaagggcgag gggatcgagc acgtgctcct
     1741 actagggatg ggcggctcca gcctcgctcc cgaggtcttc ggcttcgccc tcgaacgcaa
     1801 ggaaggctac ccggacctcg cggtcctcga tagcaccgac cccgaggccg tgctgcgcca
     1861 cgccggaagt cttgacctct cgcgcacgct cttcgtcgtc tcgaccaagt ccggcggcac
     1921 cgttgagacg ttctccttct tcaagttctt ctacaacaag gtcgcggaga tcgtgggcgc
     1981 ggagcgggcc agctcgcact tcgtcgccat caccgacccc ggcagcggtc tgcaggatac
     2041 cgccgagaaa tacggcttcc ggcagacctt cctgaacgac ccgaacatcg gaggacgcta
     2101 ctcggcgctc tcgttcttcg gggtcgtgcc ggcggcgctg gccggggtgg aagtagcgaa
     2161 gctcctgaac agcggcaagg acgccgcccg agaattcgcc ggcactgagg gagaagacaa
     2221 ccccggcgcg tggctcgggg ccgtgatggg cgagctggcc cgctccgccg ggcgcgacaa
     2281 gctgacgctg ctcgcgtcgc ccgcgctcgc accgttcggc ccctgggtgg agcaactcat
     2341 cgccgagagc accggcaagg acggcgtcgg catcctgccg gtcgccgagg agccggaagg
     2401 ctccccggag gtctacggcg acgaccggtt cttcgtgagc atgaggctgc gtagcgagga
     2461 gccggacgct ctgctcgacg agttgaagag ccaggggcac cccacggtcg aggtcctgct
     2521 ggacgatccc tacgacctgg gcggcgagat gttccgctgg gaggttgcga ccgcgatctc
     2581 cggctggagg ctcggcatca acccgttcga ccagccaaac gtcgagtccg cgaagattca
     2641 ggccaggaag atggtcgccg agtaccagga gaagggcgag ttgcccgagc ccgagccgac
     2701 ggtcgaggac ggcggaatct ccgtctactc gtccggggga gcggcgagcg tggaagaggc
     2761 actcgaaggt tttctctccc aggctcggcc cggcgactac gtgtcgttgc aggcctatct
     2821 cccgccatcg gacgagacca ccgagaccct gcaggggctg cgcgtccgct tgcgggagcg
     2881 tctcaagctc gccacgaccc tcggttacgg gccgcgcttc ctgcactcta ccggccagct
     2941 acacaagggc gacggcggca acgggctctt catccagatc acatccgacc cgcgagagga
     3001 cgcgcccatc cccgacgagg cgggcaaacc agaggccacg ctcaccttcg gggtgctcga
     3061 agccgcccag gcccttggag accggcgggc gctgctagac gtggaccggc gcgtgatccg
     3121 cttccacctc ggcggagacg tgagcgccgg cctcgacagg ctcgccaaca gcatctgatg
     3181 aaagaagacc tcgcgaagga aatgatcatc cagaaaccgt ctcgtagata gccggttcgt
     3241 aggcaggaga agaaccggca aagggaaagc ccggtctccg gcgaccattt cggcggccgg
     3301 gcatggatta ggaggaaaga tggccgaagg tccgtactta atgggcatag atttcgggac
     3361 gggtggcgtg cgggtcggcg tcttcgaccg ggaggggacg ccggtggtct tccacggcgt
     3421 cgagttcgac acgaagttcc cacgctccgg ctgggcggag caggaccccg atgtatggtg
     3481 gtccagcttc gtggaggcga cgaagaaggt gatgcaggag ggcggcatct cgccggagga
     3541 gatcgccggt atctccacgg actccacggc ctcaaccgtg ctggcgatgg acgcgaacga
     3601 caagcacctg cggcccgcca taatgtggat ggacgtgcgc gcctccgacc aggcgaagaa
     3661 gctggaagat acccacgata acgccctgaa gtactgcggg tacggggccg tctccgccga
     3721 gtggggcctc ccgaaagccc tctggctcaa ggagaacgag ccggagacct acgagaacgc
     3781 ccggcacata tgcgattgta acgactggct gatcaaccgc ctcaccggtg agtggaagtc
     3841 gtccgtcaac atcgccagcg cgaagttctt ctacgaccgc gacgagggcg gctggcccga
     3901 gaccctcctg gaggccgtcg gcttcgagga cttcctggac aagttcccgc cggacgtgct
     3961 ggacctgggc gaggtcgtcg gcggtctgcg caaggacgtg gccgacgagc tgggcctcaa
     4021 ggagggcacg ccggtcgcgc agggcgccat tgacgcctac gccggggctc tcgggctcgg
     4081 ggtggtggag ccggggacgc tcgcgctcat caccggctca tcgcacgtca tgatcgggca
     4141 ggccgcccgg cccacccacg ggcagggctt ctggggctca tacacggacg ccatgatccc
     4201 cggccagtac accgtcgagg ccgggcaggt ctccacgggt tccgtcgtgg cctggttcaa
     4261 gaacaacttc gcgtcggagg cggcaaaaga ggccgagagc cgcggcgtgg acacctacga
     4321 cgtcctcaac gagatagccg agaaagtgcc gctggggtct aacggcctga tcttcatcga
     4381 ctacttccag ggcaaccgct ccccccacac cgatccgctg gtgcgcggcg cgatctgggg
     4441 cctctcgctg ggccacactc ccggccatat cttccgctcc atcatcgagg ggatctgctt
     4501 cggcacggag agcatcttcc gcgccatgcg cgggcaccag ttcgagccgc ggctgaacgt
     4561 cgtctccggc ggtcccgcca agagcgag
//
LOCUS       DASSMB010000049        10827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1785599, whole genome shotgun sequence.
ACCESSION   DASSMB010000049 DASSMB010000000
VERSION     DASSMB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10827
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785599"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /locus_tag="VFJ72_03425"
     CDS             complement(<1..1274)
                     /locus_tag="VFJ72_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET7478552.1"
                     /translation="MFPRLSAPRLIVVAFAGLILLGTALLKIPAANNNVSWLDALFES
                     VSAVTVTGLQAVTPAKDFTPFGQGILALLIQLGGLGIMTAATVGALLVGRRLGFRDLL
                     IVREELTSPDSPGNVVRLVGQVALITLLVEVVGVVILTARFTVEGHGIGSLGLGIFHT
                     IAAFCNAGFDIFEDGMASYAGDWVVNLTLMYLIIMGGFGFPVLVNLYHYRKMRRLTLQ
                     SKLVLITTGVLLTIGVLSVAALEWTNPNTLGGQPFHTRVLMSLFQGVTPRTAGFATVT
                     YSDMRDPTLAIQIVLMFIGTAPVSTGGGIKVTTVALIFLIFLSQVRGQEEVTAFGRRI
                     PRSLIAKSLTVLSLSVLLVGGASLALMLSDNVHFLVAVFEVTSAFGTVGLSLGPGYGL
                     ATELSPFGKLLLMAVMFAGRVGPITVILALSER"
     gene            complement(1323..1487)
                     /locus_tag="VFJ72_03430"
     CDS             complement(1323..1487)
                     /locus_tag="VFJ72_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478553.1"
                     /translation="MNRRWTPSSRRDRRWGSRSGRRAARGTDRYFALIIGGFALVVIL
                     IFVAILVYGS"
     gene            complement(1499..2380)
                     /gene="sucD"
                     /locus_tag="VFJ72_03435"
     CDS             complement(1499..2380)
                     /gene="sucD"
                     /locus_tag="VFJ72_03435"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET7478554.1"
                     /translation="MAILVDNDTRLVVQGITGREGAFHTGRMLESGTQVVAGVTPGKG
                     GQDQEGVPIFNTVGEAVRETGANASVIFVPAAFSADAIFEALDAGVSTVCCITEGVPV
                     HDMLKVSDYMRMTEATLVGPNCPGMLSPGKANVSIMPRDIFTPGNVGVVSRSGTLTYQ
                     VVNELTQRGIGQSTAVGIGGDPIIGTDFIEILKKFEADPETECAVMIGEIGGNAEQLA
                     AEYIQSEMKTPVVAYIAGFTAPEGKTMGHAGAIVSGGESTAEAKSEALRKAGIRAAEN
                     PTEVGALVAEALGISKE"
     gene            complement(2381..3532)
                     /gene="sucC"
                     /locus_tag="VFJ72_03440"
     CDS             complement(2381..3532)
                     /gene="sucC"
                     /locus_tag="VFJ72_03440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET7478555.1"
                     /translation="MDLYEHQGKELLREYGLETLEGIVATTPEEARKAAEKIGGTVAV
                     KAQVLTGGRGKAGGIKVVNSPEEAEQAAERILGMDIRGHTVRRVLVEGGADIAKEMYL
                     SITVDRTAKKPLVLFSTEGGVDIEEVAETNPEAIVRMHIDPLVGLLPYQMREIQFASG
                     LDKDAAKGLGKTLSNLYKAFTGADASLVEINPLVITGDGKVLTLDSKVTVDNTSLFRH
                     PDIAELHDVEAADPQEQRAQEAGLQYVKLDGDVGILGNGAGLVMSTLDVVAQAGGEPA
                     NFCDVGGGADAEKISTALDIVTSNEKVKSVFFNIFGGITRGDEVARGLLSAIERTGLE
                     LPIVVRLDGTNAEEGRQILAENTPENVHTEETMLDAARRAVELARDGGR"
     gene            3646..4365
                     /locus_tag="VFJ72_03445"
     CDS             3646..4365
                     /locus_tag="VFJ72_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HET7478556.1"
                     /translation="MKRGGADELTGIGTAINVATVLVGGGIGTLVGARLPEGMRETAM
                     SAIGIVTLLVGVSNFLKFDNPLVPLVSVILGLVVGELLDIHGTLKRFGDYLEKRFSKG
                     ESPVSRAFVTTSLLFCVGPLTVIGSLEDGLSGDYSLLALKSALDFIAALTFSSVLGWG
                     VLLSAGTVLVVQGALTFGAGFLDPYVTDAMISATTATGGILIVGLGLMLLDLKEVRVA
                     NMLPALLFAPVLITAAPLWPF"
     gene            4443..4658
                     /locus_tag="VFJ72_03450"
     CDS             4443..4658
                     /locus_tag="VFJ72_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478557.1"
                     /translation="MENDRHWRGSPLDMGSGMDPYGIREVVIEFDNGDTDVFRPKRRD
                     EFGSYELHQIGAYVDAMAHSIRKSMRS"
     gene            4661..4891
                     /locus_tag="VFJ72_03455"
     CDS             4661..4891
                     /locus_tag="VFJ72_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478558.1"
                     /translation="MHGIERHRNIQGAPEPDQRVVRIIVRYADGRNLDFVADASRDFF
                     SEDDILELKKVFDRASSAAEWAEVTERANAGG"
     gene            complement(4929..5171)
                     /locus_tag="VFJ72_03460"
     CDS             complement(4929..5171)
                     /locus_tag="VFJ72_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HET7478559.1"
                     /translation="MAQTVMQKINELSEERERLVAREGTHHADDADHARLGKIDHDLQ
                     VLWDLRRRELAGERVDLQADYLDRYDRYTDRDGPGR"
     gene            5270..5692
                     /gene="mce"
                     /locus_tag="VFJ72_03465"
     CDS             5270..5692
                     /gene="mce"
                     /locus_tag="VFJ72_03465"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET7478560.1"
                     /translation="MLNRICHLGYAVEDVEAAARFYRDSFGVEPGEQEVVEEQGIVAT
                     MFRVGESMIELVQPTRPDSPIGKFLAKRGEGFHHVAFEVDDLEAALADLKSRGVELID
                     ETPRIGAGGSRMAFVHPKGAFGVLTELVELPHRDEGEV"
     gene            5696..7288
                     /locus_tag="VFJ72_03470"
     CDS             5696..7288
                     /locus_tag="VFJ72_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7478561.1"
                     /translation="MEVEKRKGEVRTESGIEVKSLYRPEDLDGFSYEEKLADPGEYPY
                     TRGVYPSMYRGRPWTMRQYAGFGTAKETNARFKYLTENGQTGLSVAFDLPTQMGRDSD
                     HELAQGEVGKVGVAIDSLLDMRDLFDGIPLAEVSTSMTINATASILLLLYSLVAEEQG
                     AEAKDVTGTTQNDILKEYLARGTYIYPPAPSMRVTTDMFAYCARELPRWNTISISGYH
                     IREAGSTAVQELAFTLSNAIAYVEAAVDAGLDVDQFAPRLSFFFNAHNDLFQEVAKYR
                     AARRMWAKIMRERFGATDERSLKLRFHTQTAGSSLTAQQAENNIVRTTVQALSAVLGG
                     TQSLHTNAFDEALALPTERSARIALRTQQILASEGGLTGTADPLAGSYYVESLTDAVE
                     EQAWEYIRRIDEMGGSVKAIEERFMQREIEESAFRYQREVEKEERVIVGVNRYASEED
                     PEMDLHTADEEIQERQAERLGELRESRDSAAVEKSLADLKSAAEGSANILYPMKEALA
                     NLATLGEVSDTLREVFGQYRPS"
     gene            7462..9138
                     /locus_tag="VFJ72_03475"
     CDS             7462..9138
                     /locus_tag="VFJ72_03475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET7478562.1"
                     /translation="MLLVFLTIQSSVQKAQTETEGQLSPPTGEEPTYDGGAGKKFETG
                     EIIVKIEPEASPADLRELNRENDARTEEDLPKSDVNLVDLPPDLTVKEAVDVYEQSPD
                     VEYAQPNYLLYPTKTTNDPYYARYLYGLNNTGQTMNGTAGTPDADIDAPEAWDTSTGS
                     QNTVVAVIDEGVDINHPDLRDNIWTNPGEVAADGKDNDGNGYIDDVHGWDFANNDASV
                     YDPDPITGQGDEHGTHVAGTIAAQGNNSTGISGVNWDARIMPLKFLGANGGNTLDAVE
                     AINYAVNKGVKISNNSWGGGGYSQALYDAIKNADVAGHLFVTAAGNGGADGVGDNNDT
                     TANYPSNYNLSNIISVAATNNKDTLASFSNFGATSVDLAAPGVDIVSTYPESGYGYMS
                     GTSMATPHVTGVAALLKSQDPALDDASLRSRILGSVDKKDSLQGKVATGGRLNAAAAL
                     AGTSTGASAPDTTKPVVSAVRPLAGSRTRDRTPAISATVRDDRTNLAKSNIVFYVDGR
                     RKTTFAYNTSTDRLTFTSGRLSYARHTVKIVARDAAGNVGTKIWRFTVAR"
     gene            9425..10573
                     /locus_tag="VFJ72_03480"
     CDS             9425..10573
                     /locus_tag="VFJ72_03480"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET7478563.1"
                     /translation="MLDMETETAEHLVDRTPAPPEPADGFSEAGIREALRDVRDPEIG
                     RDLISLNMIRSIEIEGSAVTVGVALTTAGCPLKHRITGDIRDRLRMIEGVEEVEVDFG
                     VMTDADRQNLLTSLHGGPSELAPAFTDESKTRIIAVVSGKGGVGKSTVAVNLAAALDR
                     AGHSVEILDADVHGSSIPVMLGSLEKPNVVDGVIFPIESASGLKFISMGNFVNEGQAI
                     IWRAPIVNKALTQLMRDVYWDEPDFIIVDMPPGTGDVALTVAQMIPKAEALVVTTPQA
                     DAARVAVKAGKMAVQAHLKVIGVVENMAHAICPCCGEEMRIFGGDGGDRVAGELDSRI
                     LGRIPILPDESGEPGRSLFPEDSTPARAFEEIAATLAATKSRKRIKVL"
BASE COUNT         2039 a         3492 c         3418 g         1878 t
ORIGIN      
        1 cgctcgctca gggcgaggat gaccgtgata ggtcctaccc gtcccgcgaa catcaccgcc
       61 ataagcagca gtttaccgaa cgggctgagc tccgtggcga gaccgtagcc gggtccgagt
      121 gacaggccga ccgtcccgaa cgccgacgtg acctcgaaga ccgccacaag gaagtgtacg
      181 ttgtccgaga gcatcagagc cagcgatgcc cctcccacga gcagcacaga gagcgagagg
      241 acggtcaggc tcttggcgat cagggagcgg gggatgcgac ggccgaacgc cgttacctcc
      301 tcctgcccgc ggacctggga gaggaagatc aggaatatga gcgcaacggt cgtcactttg
      361 atgccgccgc cggtcgagac cggggccgtc ccgatgaaca ttaggactat ctggatcgcc
      421 agcgtcgggt ccctcatgtc cgagtaggtt acggtcgcga agcccgccgt ccgcggcgtc
      481 accccctgga agagcgacat caagacgcga gtatggaagg gctggccgcc gagcgtgttc
      541 gggtttgtcc actccagagc cgcgacgctc aggacgccga tggtgagcag gacgcctgtc
      601 gtgataagga cgagcttgga ttgcagggtg agccgccgca tcttccggta gtggtacagg
      661 ttcaccagca ccggaaaacc gaagcctccc atgatgatca ggtacatcag cgtcaggtta
      721 acgacccagt cgccggcgta ggaggccatc ccatcctcga agatgtcgaa cccggcgttg
      781 cagaaggccg cgatagtgtg gaagatgccg aggccgagag acccgatacc gtgaccctcc
      841 acggtgaagc gagccgtcaa tatgacgacg ccaacgacct ccacgagcag cgttatcagg
      901 gctacctgtc cgacgaggcg cacgacgttg ccgggggagt ccgggctcgt gagctcctca
      961 cgcacgatca ggaggtcccg gaagcccagc ctgcggccca cgagcaacgc gcccaccgtg
     1021 gccgccgtca tgatccccag tcctccgagc tggatcagga gcgcgagtat cccctggccg
     1081 aagggcgtga agtccttcgc cggggttacc gcttgaagcc ccgtaaccgt taccgcgctc
     1141 accgactcga acaaggcatc gagccaggag acgttgttgt tggcggccgg gatcttcagc
     1201 agagcggtgc cgagcaggat cagacccgcg aaagcgacca cgatgaggcg cggagccgat
     1261 agtctgggga acatccgcct atcttacccg gatgccgtcg gcaacatcca cgccggcgcc
     1321 ggctacgagc cgtagaccag gatcgcgacg aagatcagga taacgaccag cgcgaacccg
     1381 ccgatgatca gggcgaagta ccgatccgtt ccccgcgccg cgcgccgtcc ggacctgctt
     1441 ccccaccgcc ggtccctccg gctgctcgga gtccagcgcc tgttcatgtc ctccgttcct
     1501 actccttgct gatacccagc gcctccgcca ccagcgcgcc gacctccgtc gggttctccg
     1561 ccgcccggat accggctttt ctgagagcct cgctcttcgc ctcggcggtg ctctcgccgc
     1621 cggagactat ggcgcccgcg tggcccatcg tcttgccttc gggggcggtg aagcccgcga
     1681 tgtaggcgac gacgggggtc ttcatctccg actgaatata ctcggctgct agctgttcgg
     1741 cgttgccccc gatctccccg atcatcaccg cgcactccgt ctccgggtca gcctcgaact
     1801 tcttgaggat ctcgatgaag tccgtcccga tgatggggtc gcctccgatc ccgacggccg
     1861 tgctctgccc gatgccgcgc tgcgtcagct cgttgacgac ctggtacgtg agcgtcccgc
     1921 tacggctcac aacgccgacg ttgcccggcg tgaagatgtc gcgcggcata atggagacgt
     1981 tagccttgcc gggcgagagc atccccggac agttcggccc gacgagcgtc gcttccgtca
     2041 tccgcatgta gtccgagacc ttgagcatgt catgaaccgg cacgccctca gtgatgcagc
     2101 agaccgtgct cacgcccgcg tccagcgctt cgaagatggc gtccgccgag aaagccgccg
     2161 ggacgaagat cacgctcgcg ttcgctcccg tctcacggac ggcctcgccg acggtgttga
     2221 agatcgggac gccttcctgg tcctggccgc ccttgcccgg cgttactccg gctacgacct
     2281 gcgtgccgct ctccagcatg cgtccggtgt ggaaagcacc ctcccggccc gtgatgccct
     2341 gcaccacgag ccgcgtgtcg ttgtcaacga gtatcgccat ttaccgtcct ccatcccgcg
     2401 ccagctccac ggcccgccgg gccgcgtcca gcatggtctc ctcggtgtgt acgttctccg
     2461 gggtgttctc ggccagtatc tgccggccct cctcggcgtt cgtgccatcc agccgcacga
     2521 ctatcggcaa ctcaagcccg gtccgctcta tcgccgagag caggccccgc gcaacctcgt
     2581 cgccgcgcgt gatgccgccg aagatattga agaagacgct ctttaccttc tcgttcgagg
     2641 tcacgatgtc gagggcggtg gagatcttct ccgcgtccgc cccgccgccg acgtcgcaga
     2701 agttcgccgg ctccccgccg gcctgcgcca ccacgtcgag cgtgctcatc accagccccg
     2761 ccccgttacc gaggatgccc acgtcgccgt cgagcttcac gtactgtagg cccgcctcct
     2821 gcgcccgctg ctcctgcggg tccgcggcct ccacgtcgtg cagctccgcg atgtccgggt
     2881 ggcggaacag gctggtattg tccaccgtta ctttggagtc gagcgtgagc accttcccgt
     2941 cgccggtgat gacgagcggg tttatctcca cgaggctcgc gtccgcgccg gtgaaagcct
     3001 tgtagaggtt cgagagcgtc ttgccgaggc ccttcgcggc gtccttatcc aggccggaag
     3061 cgaactggat ctcgcgcatc tggtacggca gcagcccgac gagcgggtct atgtgcatcc
     3121 gtactatagc ctccgggttc gtctcggcga cctcttctat gtccacgccg ccctcggtcg
     3181 agaagaggac caggggcttc ttcgccgtcc ggtccacggt gatggagagg tacatctcct
     3241 tcgcgatgtc cgcgccgccc tccacgagca cgcgccggac ggtgtgcccc cggatatcca
     3301 tgcccaggat ccgctccgcc gcctgctcgg cctcctctgg gctgttcacg accttgatgc
     3361 cgccggcctt cccgcgcccg cccgtcaaga cctgcgcctt gaccgcgacc gtgccgccga
     3421 tcttctcggc cgccttccgc gcctcctcgg gcgtggttgc gacgatgcct tcgagcgtct
     3481 caagcccgta ttctctcaac agttccttgc cctgatgctc gtagaggtcc aaacccaact
     3541 cccgcgaagc ccgtaacgac ggcccgaatt atacagagcc agccgccgat gttcgcagct
     3601 atggtccggg cgtccgtccc gtgtgccggt gcgctctgta tatacttgaa gcgtggaggc
     3661 gcggacgaac tgacgggcat cgggacagcg ataaacgtag cgaccgttct ggttggaggg
     3721 ggcatcggca cgctcgtcgg cgctcgcctg ccggagggaa tgcgcgagac ggcgatgtcg
     3781 gccatcggca tcgtgacact gctcgtcggg gtctctaact tcctcaagtt cgacaacccg
     3841 ctcgtcccgc tcgtcagcgt gatcctgggt cttgtcgtgg gcgagctgct cgatatccac
     3901 ggtactctca agcgcttcgg ggactacctt gagaagcggt tctcgaaggg ggagagcccg
     3961 gtgagccggg cgttcgtaac cacgagccta ctgttctgcg tggggccctt gaccgtgatc
     4021 ggctctcttg aggacggcct cagcggtgac tacagtctgc tcgccctcaa gagcgccctc
     4081 gacttcatcg cggcgctcac cttctcctcg gtgctgggat ggggagtcct gctctcggcc
     4141 ggaactgtcc tggtcgtgca gggcgcgctg acctttgggg ccggtttcct cgacccatac
     4201 gtcacggacg ccatgatctc cgcgactacc gccaccggcg gcatacttat cgtcggcctc
     4261 gggcttatgc tgctggacct caaggaagtc cgggtggcga acatgctccc cgccctcctc
     4321 ttcgcgccgg tcctcatcac cgccgctccg ctctggcctt tctaactttc gcccgcggcg
     4381 tcgatactgg tagtattcgc atcggtaacc gagaaccgcg gcggttagaa agggcgtggg
     4441 agttggagaa cgacaggcac tggcggggca gcccgctcga catgggctcc ggcatggacc
     4501 cttacggcat ccgggaggtc gtcatcgagt tcgacaacgg cgacaccgac gtcttccggc
     4561 cgaagaggcg ggacgagttc ggatcctacg agttgcacca gatcggcgcc tacgtggacg
     4621 cgatggcgca ctccatccgc aagagcatga ggagctagcg atgcacggga tagagcgcca
     4681 ccgcaacata cagggagctc cggagccgga ccagcgcgtc gtcaggataa tcgtgcgcta
     4741 tgccgacggg cgcaacctcg acttcgtcgc cgacgccagc cgggatttct tctccgagga
     4801 cgatattctg gaactcaaga aggtcttcga ccgcgcctcc tccgctgccg agtgggccga
     4861 ggtcacggag cgcgcgaacg ccggaggata gctgcgcgga tagcggcggt tagagagctc
     4921 agggaagtct agcgtccggg accgtcccgg tccgtgtagc ggtcatagcg gtccaggtag
     4981 tccgcctgca ggtccacccg ttcaccggca agctcccggc gacgcaggtc ccacaggact
     5041 tgcaggtcgt ggtcgatctt cccgagacgc gcgtggtccg cgtcgtcggc gtggtgggtg
     5101 ccttcgcggg ccacgagacg ctcgcgctcc tcggataact cgttgatctt ctgcataaca
     5161 gtctgcgcca tgatctcctc ctcgctcaac aagcagtgta catccgccgt cagcccgcgg
     5221 cccggtctgc tggactagaa ttgagctact actacacaga cggagggata tgctgaacag
     5281 gatctgccat ctcgggtacg cagtagagga cgtcgaagcc gccgcccgct tctaccgcga
     5341 cagcttcggc gtcgaaccgg gagagcagga ggtagtggag gagcagggca tcgtcgccac
     5401 catgttccgg gtgggggagt ccatgatcga gctcgtccag cccacccgcc ccgactcgcc
     5461 catcggcaag ttcctcgcca aacgcggcga gggattccac cacgtcgcat tcgaggtgga
     5521 cgatctggaa gccgccctcg ccgacctcaa gagccgcggc gtcgaactca tagacgagac
     5581 cccgcggatc ggcgcgggcg ggagcaggat ggccttcgtt catcctaagg gggccttcgg
     5641 cgtgctcacg gagctagtag aattgcccca cagagacgag ggagaggtgt aagacgtgga
     5701 agtggagaag cggaaaggcg aagtccgcac ggaatccggc atagaggtaa agtccctcta
     5761 ccgccccgaa gacctggacg gcttctctta tgaggagaag ctcgccgacc ccggcgagta
     5821 cccctacacc cggggcgtct atccctcaat gtatcgcggc cgtccctgga ccatgcgcca
     5881 gtacgcgggc ttcggtaccg ccaaagagac caacgcccgc ttcaaatacc tgaccgagaa
     5941 cggccagact ggactctccg tcgccttcga cctccccacc cagatgggcc gcgactcgga
     6001 ccacgagctg gcgcagggcg aggttggcaa agtaggcgtc gccatagact cgcttttgga
     6061 catgcgagac ctcttcgacg gcatcccgct cgcggaggtc tctacatcca tgaccataaa
     6121 cgccaccgcc tcgattctgt tgctcctcta ctcccttgtc gccgaggagc agggcgccga
     6181 ggcgaaggat gtgaccggca ccacccagaa cgacatcctc aaggagtatc tggcgcgcgg
     6241 cacctacatc tatccgccag caccgtccat gagggtcacg accgacatgt tcgcctactg
     6301 cgcgagggag cttccgcgat ggaacaccat ctccatctcc ggctaccaca tccgggaggc
     6361 gggcagcacg gctgtgcagg agctcgcctt cacgctgtct aacgccatcg cctatgtgga
     6421 ggcggcagta gatgcggggc tcgatgtaga tcagttcgcg ccgaggctct cgttcttctt
     6481 caacgcgcac aacgacctgt tccaggaggt cgccaagtac cgcgcggcgc gcaggatgtg
     6541 ggcgaagata atgagggagc gtttcggcgc gaccgatgag aggagcctta agctccgttt
     6601 tcacacgcag accgccggct cctccctgac cgcccagcag gccgagaaca acatcgtccg
     6661 caccaccgtg caggcgctct cggccgtgct cggcggcacc cagagccttc acaccaacgc
     6721 tttcgatgag gctctcgcgc ttcctaccga gcggagcgcg cgcattgccc tgcgaacgca
     6781 gcagatactg gcgagcgagg gcggcctcac cggcaccgca gaccctttgg ctggctccta
     6841 ctacgtcgag tcgctcacgg atgccgtgga agaacaggcc tgggagtaca tccggcgcat
     6901 agatgagatg ggcgggtcag tgaaggccat cgaagagcgg ttcatgcagc gcgagatcga
     6961 ggagtccgcc ttccgttacc agcgcgaggt agagaaagag gagcgcgtta tcgtcggcgt
     7021 caatcgctac gccagcgaag aagatcccga gatggacctc cacacggccg atgaggagat
     7081 ccaggagcgg caggccgagc ggctaggaga gctgcgagag agccgcgact ccgctgctgt
     7141 cgagaagtcg ctggcagacc tcaagagcgc cgccgaagga tcggccaaca tcctctaccc
     7201 gatgaaggaa gccctggcga acctcgccac gcttggagag gtctccgata ctctgaggga
     7261 agtatttgga caatatcgcc cttcgtagcg gaattcgacg cgtttgtgaa taaagagact
     7321 taagtttttc gccgtttgtc cgataacctg cggggtaaca cttatacaca agcgtagaag
     7381 ccagcgtttc aaaggatttt tgaggtattt atggcgagta agtggagaat aggcgctgta
     7441 ctggttggta cggtagcggc catgctactg gtatttctga cgattcaaag ttccgtgcag
     7501 aaggctcaga ccgagacgga ggggcaactc tcgccgccga caggcgagga gccgacctac
     7561 gatggcggcg cgggtaagaa gttcgagaca ggtgagatca tcgtaaagat agagccggag
     7621 gcttccccgg cggacctgag ggaactaaac cgcgagaacg acgcccggac agaggaggac
     7681 cttccgaaga gcgacgtgaa ccttgtggat ctccccccgg acctcacggt aaaagaggcc
     7741 gttgacgttt acgagcagtc gccggacgtc gagtacgcgc agcccaacta cctcctctat
     7801 ccgaccaaga cgacgaacga cccgtactac gcaaggtatt tgtatggcct gaacaatacg
     7861 ggtcagacca tgaacgggac cgcgggcacc cccgatgcgg acatcgatgc gccggaagcc
     7921 tgggatacgt ccacgggcag ccagaacacg gtagtcgcgg tcattgacga gggagtggat
     7981 atcaaccatc ccgatctccg ggacaacatc tggactaacc ccggtgaggt cgccgccgac
     8041 ggcaaagaca acgacggcaa cggatacatt gacgacgtac acggctggga cttcgccaac
     8101 aacgacgcga gcgtttacga ccccgacccc ataaccggcc agggcgacga gcatggcact
     8161 cacgtggccg gcaccatcgc cgcccagggt aacaacagca cgggcatctc cggcgtcaac
     8221 tgggacgcgc ggataatgcc gctcaagttc ctcggcgcga acggtggcaa taccctcgat
     8281 gcggtcgagg ccatcaacta tgcggtcaac aagggtgtca agatcagcaa caactcctgg
     8341 ggcggtggtg gatacagtca ggcgctctac gacgcgataa agaacgctga cgtcgccgga
     8401 cacctgttcg tgacggcggc cggcaatggc ggagcggacg gtgtcggtga caataacgac
     8461 accacggcga actacccgtc gaactacaac ctgtcaaaca tcatctccgt cgccgccacc
     8521 aacaacaagg atacgctggc ctcgttctcc aacttcggcg cgacgtcagt ggacctggcg
     8581 gctccaggtg tggacatcgt gagcacctac ccggagagcg gctacggtta catgagcggg
     8641 acctcgatgg ctacgccgca cgtgacaggc gtggcggccc tgctcaagag tcaggacccg
     8701 gcccttgacg atgcatcgtt gcggtcgcgg atactcggtt cggtggacaa gaaagacagc
     8761 ctgcagggca aggtggcgac cggcggacgt ctgaacgccg ctgcagcgct ggcggggact
     8821 tcgaccggcg catccgctcc ggatactacg aagccggtgg tctccgctgt ccggccgctg
     8881 gcgggctcca ggacacggga taggactccg gcgatcagcg cgaccgtccg ggatgatagg
     8941 acgaatctcg ccaagagcaa catcgtgttc tacgtggacg gacggagaaa gactacgttc
     9001 gcttacaaca catccaccga ccgcctaacc ttcacctcgg gacgcctttc atacgcccgg
     9061 cacacggtca agatcgttgc tcgggatgcg gcaggcaacg tgggcacgaa gatatggaga
     9121 tttaccgtag cccggtaagc cttaaggcaa gtcaggactg gagggatcgg gactttccgg
     9181 tccctcttcc tctgggtgcg ggaggtgcga tccgtggtcc tctggctcgg actgatccgg
     9241 ggtagcgtat ggtaacattg gcgcgcaaac gcgggtttta gcgggatgtt cgaggttttc
     9301 cacacgccgg ccgcgaagcc ggaggaggtt ttcagacatg gttgactggt ttcgcagaag
     9361 gacgggccgg aacgaagaca ctactagctc cgcgtcggcc gaggcgggtc cggaggagcg
     9421 agagatgctc gacatggaga cggagacggc ggagcacctc gtagacagga cccccgcgcc
     9481 gccggaaccg gcggacggtt tctcggaggc ggggatccgg gaggctctca gagacgtgag
     9541 agacccggag atcggacgcg acctcatctc cctgaacatg atccggtcca tcgagatcga
     9601 aggttcggca gttaccgtcg gcgttgccct cactaccgcc ggttgcccct tgaagcaccg
     9661 cattacgggc gatatccggg accggctgcg gatgatcgag ggagtcgagg aggtcgaggt
     9721 ggacttcggc gtaatgaccg acgccgaccg gcagaacctg ctcacctcgc tgcacggtgg
     9781 gccgtcggag ctggcccccg ccttcacgga cgagtcgaaa acgaggatca tcgcggtcgt
     9841 cagcggcaag gggggcgtgg gcaagagcac cgtcgccgtc aacctcgccg ccgcgctcga
     9901 ccgggcgggt cactccgtgg agatcctgga cgccgacgtt cacggttcct ctatcccggt
     9961 aatgctcggc tcgcttgaga agccgaacgt ggtggacggc gttatcttcc cgatagagtc
    10021 cgcgtcgggt ctgaagttca tctcgatggg caacttcgtg aacgaggggc aggccatcat
    10081 ctggcgcgct cccatcgtga acaaggccct cacgcaattg atgcgcgacg tctactggga
    10141 cgaaccggac ttcatcatcg tggacatgcc gcccggcacc ggcgacgtgg ccctaacggt
    10201 ggcccagatg atcccgaagg ccgaggcgct ggtcgtcacc acgccccagg ctgacgcggc
    10261 gcgagtagcc gtgaaagccg ggaagatggc cgtgcaggcg cacctgaagg tgatcggtgt
    10321 cgtggagaac atggctcatg cgatctgccc ctgctgtggc gaggagatgc ggatctttgg
    10381 cggcgacggc ggcgataggg tagccggcga actcgacagc cgtattctgg gccgaatccc
    10441 gatcctcccg gacgagagcg gcgagcccgg ccgttccctg ttccccgagg attccacacc
    10501 cgcccgggcc ttcgaagaga tcgcggcgac cctcgccgcc accaagtccc gcaagcgaat
    10561 caaggtttta taggaccgtc tcgcgggcgg ccctgtgggg accgcaaggt ctgtgacgcg
    10621 cggtgatgaa cggcctgttt tcgcggcgct aaatgccgtc cgggtaatgc catagaccga
    10681 ccttttgggg acattgcagg tgctccctgt gtggaccatc agcatcaggt cgagcgcgaa
    10741 tgaagtagtc ccgcgatgag cagctcgacc atgcggcggg cgtcgtagtg ggggtcgttg
    10801 tcggcgccga tgcagaggtt cccgacg
//
LOCUS       DASSMB010000050         4527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_644759, whole genome shotgun sequence.
ACCESSION   DASSMB010000050 DASSMB010000000
VERSION     DASSMB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644759"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="VFJ72_03485"
     CDS             complement(<1..1157)
                     /locus_tag="VFJ72_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HET7478564.1"
                     /translation="MSITITDVVARDIRFPTSESLDGSDAMNQAPDYSAAYVTLTTDA
                     NLEGHGLTFTIGRGNELCVAAIESLSPLVIGETLESFTRDMGAFWRHITGDSQLRWVG
                     PEKGVIHLATAAIVNAVWDLWAKTEAKPVWKLLADMSPEEVVRCIDFRYITDALTPEQ
                     ALEILRRNEGTKAAREVEMLKDGFPAYTTSAGWLGYSDEKLRRLCREAVEAGWTHFKM
                     KVGGNVEDDARRAGIIREEIGADRKLMMDANQVWDVPEAIRNMERLAEFDPHWIEEPT
                     SPDDVLGHAAIARAVAPIGVATGEHCQNRVVFKQLLQADAISFCQIDSCRLGGVNEVI
                     AVLLMAARFGVPVCPHAGGVGLCEYVQHLSIFDYLYVGASLENRILEYVDHL"
     gene            complement(1172..1807)
                     /locus_tag="VFJ72_03490"
     CDS             complement(1172..1807)
                     /locus_tag="VFJ72_03490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7478565.1"
                     /translation="MRDATRHTVEAGYDRMAEAYLATKDPEDPTTISALEDLAGYLPP
                     GASVLDLGCGAGVPATRWLARRGFAVTGVDFSGKQLELARRLVPDAVFIRADMTELDL
                     APETFDAVVALHSIIHVPRLDHPTLLGSIHRWLRPGGVFLATLTMTDFEGEDPDWEGW
                     GAAMRWSHYDRETNVSLLREAKFEVVYAEPRTGGGTGNTPETWLWVLARKD"
     gene            complement(1804..2562)
                     /locus_tag="VFJ72_03495"
     CDS             complement(1804..2562)
                     /locus_tag="VFJ72_03495"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HET7478566.1"
                     /translation="MDRLEGKRAIVTGGGSGIGRAIALRLASEGARVTLADLDEDAAE
                     NVADELDGEGHLVQPTDVTDAADVEALIARVVDEWGGLDVMVNNAGVSIAATAVDTSE
                     EDYDRVMDVCVRGTFLGMKYAIPAIRDSGGGSVVNMSSIAALVGLVDRAIYCAAKGAI
                     LSMTRAAAIDHVEEGVRVNCIAPGTVDTPWVARITAGYDDPEEAREKMKARQPHGRLV
                     TPEEIAAMAAYLASDEAGSVIGACMVVDGGMTAR"
     gene            2662..2991
                     /locus_tag="VFJ72_03500"
     CDS             2662..2991
                     /locus_tag="VFJ72_03500"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7478567.1"
                     /translation="MNPYADWIQALPQVDTPFTGLEGRMISGPDGQAVFFRAEEDFEV
                     PPHSHGAQWGIVVTGRLHLTVEGEPAVYEPGETYDIPAGAEHAARLEAGTCVIDIFQD
                     PDRYSPK"
     gene            complement(3091..4200)
                     /locus_tag="VFJ72_03505"
     CDS             complement(3091..4200)
                     /locus_tag="VFJ72_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET7478568.1"
                     /translation="MSMEGSNNLRIVILALLVGFAALLVATVSFGARAQAAPVSDPLT
                     KTLSLDSVCDVSLVAVGKGVPGDPSVIEVPTGWTVKLLDDPSGDGTPGVYECNPVAGP
                     VFDPKTKTTVTGLVSGDGISVDAPGVTFDLNGHSIASTKDILAASAPADVENAGVSIG
                     ANNVVVTNSSTVRSVVSNFTTNFSVAKSNDSRISGSKLADGTYNLKGGNAHGGASLSV
                     DKSLRLTVDTVELSDLSGDGTSTEGVDWKRCSGPTGTIQNSLVQGDLGGMRFKGCQSG
                     GLSVLNNHVIGHGGSGVEFMRDNKGATVSGNTIEGSTAGDGVTVRAPAESISITSNTI
                     QNNAGFGIQTIGAVKSITTTPNTFANNALGNVGVK"
BASE COUNT          717 a         1483 c         1438 g          889 t
ORIGIN      
        1 aggtggtcca cgtactccag gatgcggttc tccagcgagg cgccgacgta caggtagtcg
       61 aagatggaga ggtgctggac gtactcgcag aggccgacgc cgccggcgtg cgggcagacg
      121 gggacgccga acctggcggc catcagcagc acggcgatca cctcgttcac gccgccgagg
      181 cggcaggagt ctatctggca gaagctgatc gcgtcggcct ggagtagctg cttgaagacg
      241 acgcggttct ggcagtgctc gccggtggcg acacctatgg gggcgacggc ccgcgctatg
      301 gcggcgtggc cgagcacgtc gtcgggactg gttggctcct cgatccagtg cgggtcgaac
      361 tccgccaggc gctccatgtt ccgtatagcc tctggcacgt cccagacctg gttggcgtcc
      421 atcatcagct ttctatcggc cccgatctcc tcccggatga tgcccgcccg ccgggcgtcg
      481 tcctcgacgt tgccgccgac cttcatcttg aagtgcgtcc acccggcctc gaccgcctca
      541 cggcacaggc gccgcagctt ctcgtccgag taacccagcc acccggcgct cgtcgtgtag
      601 gccgggaagc cgtctttgag catctcgacc tcgcgggcgg ccttcgtgcc ctcgttgcgg
      661 cgcagtatct caagcgcctg ctcgggggtt agggcgtcgg tgatgtagcg gaagtctatg
      721 cagcggacga cctcctcggg gctcatgtcc gcgaggagct tccagacggg cttcgcctcg
      781 gtcttcgccc acaggtccca cacggcgttg acgatggcgg ctgtagccag gtggatcacg
      841 cccttctccg gcccgaccca acggagctgg ctgtcgcccg tgatgtgccg ccagaacgcg
      901 cccatgtccc tcgtgaaaga ctccagtgtc tccccgataa cgagcgggct cagagactct
      961 atcgccgcga cgcacagctc gttgccccgg ccgatggtga aggtaaggcc gtgaccttcg
     1021 aggttggcgt cggtcgtcaa agtgacatag gcggcggagt agtcgggagc ctggttcatc
     1081 gcgtcggagc cgtccaggct ctccgaggtc gggaaccgga tgtcgcgggc tacaacgtcg
     1141 gtaattgtga tcgacatgcc tcttcctttc gtcagtcctt gcgggccagt acccagagcc
     1201 aggtctctgg agtattgccg gtgccgcctc cggtgcgcgg ctccgcgtac acgacctcga
     1261 acttcgcctc ccgcagcaac gatacgttcg tctccctgtc gtagtggctc cagcgcatgg
     1321 cggctcccca gccttcccag tccgggtctt ctccctcgaa gtccgtcatc gtcagggtcg
     1381 ccaggaagac tccgcccggc ctgagccagc ggtggatgct accgagcaga gtggggtgat
     1441 ccaggcgcgg cacgtgaatg atggagtgga gggcgacgac cgcatcgaag gtctccgggg
     1501 cgaggtccag ctccgtcatg tcagcccgga tgaagaccgc gtccgggacc agcctgcggg
     1561 ccagctctag ctgctttccg gagaagtcca cgccggtcac ggcgaaccct cgccgcgcga
     1621 gccagcgcgt cgccgggacg cccgcgccgc agcccaggtc gagcacggag gctcccggtg
     1681 gaaggtagcc cgccaggtct tcgagcgcgg agattgtcgt cgggtcttcg gggtctttgg
     1741 tggcgaggta ggcttcggcc atccggtcgt agccggcctc gacggtgtgg cgggtagcgt
     1801 ctctcaccgg gcggtcatgc cgccgtccac gaccatgcac gccccgatga cggagccggc
     1861 ctcgtcggat gcgaggtagg cggccatagc ggcgatctcc tcgggcgtga cgagacgccc
     1921 gtgcggctgc cgggccttca tcttctcgcg ggcctcctcc gggtcgtcgt agccggctgt
     1981 gatgcgcgcc acccagggcg tatctaccgt gccgggcgct atgcagttga ccctgacgcc
     2041 ctcctcgacg tggtctatgg ccgctgcccg cgtcatcgag aggatggcgc ccttcgccgc
     2101 gcagtagatg gcccggtcca cgaggcccac aagggccgca atggaggaca tgttaaccac
     2161 cgacccgcct cccgagtccc ggatcgccgg gatggcgtac ttcatgccga ggaaggtccc
     2221 ccgcacgcac acgtccatga cccggtcgta gtcctcctcg gaggtgtcca cggcggtcgc
     2281 ggcgatgctc accccggcgt tgttgaccat cacgtccagg ccgccccact cgtccacgac
     2341 gcgggcgatg agggcttcca cgtcggcggc gtccgtgacg tcggtgggct gtacgaggtg
     2401 gccttctccg tccagctcgt cggcgacgtt ttcggcggcg tcttcgtcga ggtcggctag
     2461 ggtcacgcgg gcgccctcgg aggcgaggcg gagtgctatt gcgcgtccga tgcccgagcc
     2521 tccgcccgtc acgattgcgc gcttgccttc gagccggtcc atcgccctca cccccgcagt
     2581 cgctcacccg tgtgagcggt cagtataatg acgcccgcgc cggaggcgca aacgctaaga
     2641 gacatcggga aaggagtcgt tatgaaccct tacgctgatt ggatccaggc cctcccccag
     2701 gtagacaccc cgttcaccgg cctggaggga cgtatgatct ccggccccga cgggcaggcc
     2761 gtcttcttcc gggccgagga ggacttcgag gtgccgcccc actcccacgg cgcccagtgg
     2821 ggcatcgtcg tgaccggccg cctgcacctg acggtggaag gtgagcccgc cgtctacgag
     2881 cccggtgaga cctacgacat acccgccggc gcggagcacg ctgcccgcct cgaagccggc
     2941 acctgcgtca ttgacatctt ccaggacccg gaccgctaca gcccgaagta gcttaccgcg
     3001 gccgcggcga gagaaggacc ggagcgaagt gccccggccc tttagtcttc cgtttgcccg
     3061 gttgctactg caaaggacct ccggcaacct ttacttcacg cccacgttgc ccaaagcgtt
     3121 gttggcgaac gtgttcggcg tggtggtgat gctcttcacg gcgcctatcg tctggatccc
     3181 gaatccggca ttgttctgga tggtgttgct ggtgatcgag atggactctg cgggagcacg
     3241 aactgttact ccgtcgccgg cggtactgcc ttcgatagtg ttgccgctga cggtggcgcc
     3301 cttattgtct cgcatgaact ccacgccact cccgccgtgg ccgattacgt ggttattcag
     3361 gaccgagaga ccgccgctct ggcaaccctt gaaccgcatg ccgccgaggt cgccctggac
     3421 caggctgttc tggatggtgc cggtgggacc ggagcaacgc ttccagtcaa cgccctcggt
     3481 gctggtgccg tcgccgctca ggtcggagag ctccacggta tcgactgtga gtctgagtga
     3541 cttgtctacc gagagggatg ctccgccgtg cgcatttccg cccttgaggt tgtaagtgcc
     3601 atcggcaagc ttagagcccg agattctcga gtcgttggac ttcgccacgc tgaagttggt
     3661 ggtgaagttg gagacgaccg accggacggt gctgctgttg gttaccacga cgttgttcgc
     3721 gccgatggag acgccggcgt tctcgacgtc cgcaggagcg ctcgccgcca gaatatcctt
     3781 ggtgctcgcg atgctgtgac cgttgagatc gaaggtgacg ccgggagcgt ccacgcttat
     3841 cccgtcgccc gacactaggc ctgttacagt ggtcttggtc ttcgggtcaa agaccggacc
     3901 ggccaccgga ttacactcgt aaaccccggg cgtgccatcg ccggaagggt cgtcaagtag
     3961 cttgacggtc cagcctgttg ggacttcgat cacgctcgga tcgcccggta ctcctttgcc
     4021 caccgctacc agcgacacat cgcagacgct gtcgagcgaa agcgtcttgg tcagcggatc
     4081 gctgaccggg gccgcctgag cccgcgcgcc gaacgagacc gtcgccacca gcaatgccgc
     4141 aaagccgacc agtaaagcca gtattacgat tcgtaagttg ttgctgcctt ccatgctcat
     4201 gtctttcctg ctttcctgag tgcctttcta ctttcctcgt gtgcctgttg cctgagaggg
     4261 tgccttgtgc ttcctttata taggtggacg tttggtccac tcgaaaaccc tcgccagggt
     4321 ggctaaattg tctgctgctc ggctctcctt ttacctccct gatctccact gctatgccga
     4381 tctagaggaa ggatgaagca cagtgcctcc gaacacctct gtaagatgag cgatttatta
     4441 ctaacgaaga tgagccaaat gtttttagcc catctcggct agctcaaatt gcgtatctga
     4501 agaatagtag atgttcttgt tatttct
//
LOCUS       DASSMB010000051        16373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1659045, whole genome shotgun sequence.
ACCESSION   DASSMB010000051 DASSMB010000000
VERSION     DASSMB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16373
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659045"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VFJ72_03510"
     CDS             complement(<1..552)
                     /locus_tag="VFJ72_03510"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HET7478569.1"
                     /translation="MIDGERIWRRIKEIGEIGRQEAGGVTRLSITAEERVAKDRVISF
                     MEEAGLSVREDAAGNLFGRREGRDSDAPVVLIGSHVDSVYNGGDFDGPLGVLAGIEVL
                     HTMNERGVQTERPVEVVAFTDEEGARFSFGMIGSRALAGKLDAGDLRHEDADGVSIAE
                     AMRANGLDPDLIGEAARSEGSVAA"
     gene            complement(557..1366)
                     /locus_tag="VFJ72_03515"
     CDS             complement(557..1366)
                     /locus_tag="VFJ72_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7478570.1"
                     /translation="MERRSLGSSGIDAPVVGLGTWQVLDVRGPEEYARHEVVRTALEA
                     GADLFDSSPMYGEAERVLGDALRNLGRERAIVATKVWHSDDREAEGQIQAALGYFGGY
                     VDLYQVHNLVAVEKRLDTLHRLKEEGRVRSIGATHYSRSAFGDLTHVMRSGRVDFVQV
                     PYNAADTSVTEEVLPLAEELGLGVIVMVPLGSGRLVRNAPPSESLEPLREFGVETWAQ
                     ALLKYVLSDRRVSCAIPATSSPDRMSENAAAGDPPWFGDEEREYVARLARR"
     gene            complement(1368..1838)
                     /locus_tag="VFJ72_03520"
     CDS             complement(1368..1838)
                     /locus_tag="VFJ72_03520"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7478571.1"
                     /translation="MKNPPVIERLEIRYQDLDPYGHVNNTIYLVFFETLRMAYFQALA
                     RKTGLDLVLGDIPGARYVVAETSVRFKVPIFLDDTLHGAASIRTVGNRSYGMDFELRT
                     GESFETGTLAAEGSAAQVFFDPVKNAVQSRPDWFLSAVAGLEGRPEESFAPEGR"
     gene            1930..3264
                     /locus_tag="VFJ72_03525"
     CDS             1930..3264
                     /locus_tag="VFJ72_03525"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7478572.1"
                     /translation="MEYEEYLDQGETLAEEGLIEEALSRFEQALEAAPENPEVIEAVG
                     RALLNLGRLEEAEASFFDALEIDPEWAAPRMGLALLAMRREEPFKVVHHLERAIEADP
                     EYPDAYVELGRYYGMMSEPALAKATFERWTLRYPEDADMHINAGLTAFDAADYAQALE
                     FFEKALEVAADGEQKSGARTFRANTLDMLGRYEEALSEYERVIKYAPDWWEAHANLGI
                     CHARNGHFAEAEAALRAGLAACPGSPEIRDELAAHLLGEDGDLEEALALAEEAVALGR
                     DEVRHLFTLGEVRLALDDEAGAQEAYEAVLGLDPEDPGAHLELGLLHERRGEVAEAEE
                     HFMEALKGDPGNPRALYSYASLYYATDDLETAEELLVRAIAVDAEYSPALSALASIRA
                     RRGDYGIALDYIEKAVEAGERDVEHFRSAIEFAPLRADPRFRGLLSRMTSGN"
     gene            3271..3495
                     /locus_tag="VFJ72_03530"
     CDS             3271..3495
                     /locus_tag="VFJ72_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478573.1"
                     /translation="MPATLAFGLFLTAVGLAALTFGLYALLRGGKEHRGGGIGPISER
                     GIHVIAGIRMTIIGAASLAAGLYFLWSYWS"
     gene            complement(3521..4123)
                     /locus_tag="VFJ72_03535"
     CDS             complement(3521..4123)
                     /locus_tag="VFJ72_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7478574.1"
                     /translation="MRLLVDEVEDIVARVGAHPVWGYKHCLRVYALAEEIGASEHASY
                     DSEILRIAALLHDVGLYKAYALREASDHAKRSSAVAGRILKDLDYPPQAARVVLDAIE
                     HHSPGTPPGATTEAALLKDAVALDYLGAIGVSRVLAMVGVEEDVPDIPAAIWHAESLR
                     RQIPDLLIFETSKAIAAERVRELDDFLQDLRNATDRLKLL"
     gene            4323..5873
                     /locus_tag="VFJ72_03540"
     CDS             4323..5873
                     /locus_tag="VFJ72_03540"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7478575.1"
                     /translation="MLKLGLLGSAALMLPVERQARTDLSIKNRIPESQLPRPFQVPFA
                     VPPVARPVKRTATTDVYQMSMRAVQAQILPGFPKTTVFGYDGLVPGPTILALAGRPVL
                     IRHINRLPALSPFNTPTTTSVHLHGSPSLPQYDGYASDVTAPGQYKDYRYPNVLNSAC
                     TFWYHDHAVHHTAENAYMGLAAQYHLHSDAELALPIPHGRYDVPLTIKDAIFTTNGSL
                     VFDDNDHSSLFGDVILVNGRPWPVMKVERRKYRFRVLNASLSRSYRLALDSGEPFTII
                     GTDDGLMPAPQRVQTFRLAMAERYEVVIDFARHKIEDRIILRNLSNPNNEDYPSTGRV
                     MMFEVASNATDTKNNTVPAVLNPDNATMNLNETQAVRTRRMAFERDGGQWTINGSTWD
                     DVIKSGFEFVIANPGLGDVEIWEVSNDSGGWFHPVHIHLIDFRVLDRNGKPPFPYEIG
                     PKDTVYVGEDEKVRLIMKFRPNRGRYMMHCHNLVHEDHDMMTQFEVGQGGNDPFSAPA
                     RDLPAPSL"
     gene            complement(5870..6820)
                     /locus_tag="VFJ72_03545"
     CDS             complement(5870..6820)
                     /locus_tag="VFJ72_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HET7478576.1"
                     /translation="MRVASLLPSATEMVHFAGAGDTLIGVTHECDYPPGVEDLPRLTS
                     SKIHAGRMTSAEIDAAIGRILTDGSSIYALDAKLLEDLTPDLLITQGLCEVCAVSMDL
                     VERAVAGMENRPEILSLNPTSLDDVLEDAARVGEALGRGNEVHDNVAALRERLARVEE
                     TVAGLPRPRVGCIEWLDPLFSAGHWVPEMVRLAGGEEVLAAPGEASARITWEQVFEAA
                     PEVLVLMPCGFGAERAAREARVLPDLPGWKDLPAVEDGRVWVVDANSYFSRPAPRLVR
                     GVEVLGHILHPEALPDTPALVEAARLSVPDPDAAGSSEVS"
     gene            complement(6833..7942)
                     /locus_tag="VFJ72_03550"
     CDS             complement(6833..7942)
                     /locus_tag="VFJ72_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7478577.1"
                     /translation="MGGSFKIGRVIGIEVKVHWTFFLLLAFFAYLGYQGAGTVLGALE
                     TSGIIVALFFCVLLHEFGHSVVAQRLGIEVPDITLLPIGGLARLKNLPEKPWDEVKIA
                     IAGPLVNVVLAPVFFFVAFALGADLFNPANVLKGTASLGQVFAYLGIVNVALVVFNMI
                     PAFPMDGGRVLRGLLATRLGPVRATDIASTVGQLFAVGFFMVGLFTGGVILMLISVFI
                     FFGASGEAQMVRQRETMRGLAVSDVMGTRRRTETVTPYHTFGQVFDMVIHGYQEDFPV
                     VDEDGRLVGLITRSEIMSAAHSPQKFLNVRDLMKTDFPTISPEADLFKDGNRLLQESG
                     LRAIPVVQDGDLVGLLTIDDIGQAALLRDVRNQKY"
     gene            8019..9761
                     /locus_tag="VFJ72_03555"
     CDS             8019..9761
                     /locus_tag="VFJ72_03555"
                     /inference="COORDINATES: protein motif:HMM:NF011539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HET7478578.1"
                     /translation="MEERTRNYQLTTEAEQLPRIAETLKEAEAIGLDTETTGLSPRDG
                     RTRLLQLATPEETYVIDVFEVGDLSPLKDALEGGPVKVLHNAKFDHAFMWAEHDISLS
                     PIFDTMLAAQLLSGGDYDKSYSLESVAQKYLDAELDKSGQRSDWSGALSDRQIEYAAR
                     DAEVLLPLRERLAAALAEDKLGTVSKIEFGAVAAISEMELAGIKLDVAKWKELEITVR
                     ERRDRAAEHLESFFPEPEGVLPLEGLGPRLNLNSPQQITDAFRSLGIDLPDTKVWTLL
                     KVDHPAAKALLEYRELQKKLGTYLETYPKFIHEKTGRIHANFLQCRVPTGRLACTNPN
                     VQQIPHEDEFRSCFVAEQGNVLVIADYSQIELRILAEVSEDPAFVTAFQNGDDLHRVT
                     AATMYGVPMEEVTKDQRSDAKRINFGLMYGRGAKSLSAQLGASEERGRQLIEEYFANY
                     PKVQRFLQETASKATKTRRLRTLSGRLRKFQDAPVSDDRGALRREAMNFPIQGASADI
                     AKLALIYIRKELKDLDARLINSIHDEFVIECAEELAEEVSERTRSAMIRAGEVLLKKV
                     PVEVEVGVSREWRK"
     gene            complement(9952..11334)
                     /locus_tag="VFJ72_03560"
     CDS             complement(9952..11334)
                     /locus_tag="VFJ72_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7478579.1"
                     /translation="MRTVPSSTTVAPAASNIHKIKHIVIIMQENRSFDSYFGTFPGAD
                     GIPMKNGTPTVCVPDPQASRCVKPFHDPKDLNYGAPHRAADASQDINGGKMDGFIRQE
                     IAGRKRSCQNRFDPACGQSSKDTPPDVMGYHDGREIPNYWSYARHFVLQDHMFESNSS
                     WSLPAHLYMVSGWSATCSVPGDPMSCRNALQRPEPPPDSSKKARQGRSQVHPPDYAWT
                     DLTYLLHKNNVSWGYFVSRGKEPDCRNDEMFCEHLNQSSRTPGIWNPLPYFDTVKGNG
                     QTGNIQNVSNFYTDAKKGNLPAVSWIVPDGKNSEHPPALVSKGQSYVTGLINAAMKSP
                     DWSSTAVFLAWDDWGGFYDHVKPPVVDENGYGIRVPAMVISPYARQGYIDHQTLSFDA
                     YLKFIEDDFLNGQRLDPQTDGRPDPRPDVRENAPQMGNLVKDFDFNQKPRSPMILPEH
                     PNPGPVPGKG"
     gene            complement(11641..12567)
                     /locus_tag="VFJ72_03565"
     CDS             complement(11641..12567)
                     /locus_tag="VFJ72_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7478580.1"
                     /translation="MPQATEGLEVKTGEHSYVGRIRDVAGAVPPTSLVLLAILSVQLG
                     AAIAKGLFEDLGPGGTVFLRISFAALVLGLIWRPRIRGYTRREYLIALLFGLTLAAMN
                     FSLYLSIDRIPLGVAVTLEFAGPLGVAVAGSRRLLDLLWVILAAAGILLLAPLGVLGS
                     SALDPVGVAFALLAGLFWASYILLSARTGSVFPGGTGLVIALCIGTVILLPVGIAGGG
                     YALLDPRLLIAGFGVAMLSAAVPYSLEMKALRKVPARVFGVLMSLEPAVAALVGFIVL
                     GERLGLKTILAILFVTVAAAGASRFGAYGQDA"
     gene            complement(12609..13787)
                     /locus_tag="VFJ72_03570"
     CDS             complement(12609..13787)
                     /locus_tag="VFJ72_03570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478581.1"
                     /translation="MNIRLFVLALGTFAIGTGSFVFAGLLEGVAEDLSVSVGAAGHLV
                     TVFAVTYAVSSPVLATFTGAVSRRRLLIVSLTLFSVANALAVVAPTFELLLLCRVLAA
                     GGAAAFTPTAAAVAASIAPPGGRGKALSIVTGGLTVSFVVGIPLGSVIGTYAGWRTTF
                     VMVGVMGLAAVLGIRALLPDVEGPPPVSLRERVGVLKHPAIVAALGLTAFGMMGGFVA
                     FTYISPLLGTLTGFGGGGVSGLLFLFGVAALFGNSLGGYGADNAGYGRLMAAILIVLS
                     FSLLALSGLFVVAGSALAIAGVVAVLAAWGIAGFAINPLQQYRVIQLAPTQSNVALSL
                     NASAIYLGQGVGASIGSLVLGHGTLATLGWTGAICSATALAVLFIGTRRPVPEAAAKG
                     "
     gene            complement(13893..15491)
                     /locus_tag="VFJ72_03575"
     CDS             complement(13893..15491)
                     /locus_tag="VFJ72_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7478582.1"
                     /translation="MEEGHRWWERGVIYQVYPRSFQDYDGDGVGDLPGITQRLEYLQW
                     LGVDAVWISPFYPSPMADFGYDISDYTGVHPMFGTLEDFDALVAEAHRLGLKVILDYV
                     PNHTSSEHPWFVESRSSRENPKRDWYIWRDPAPDGGPPNNWVASFGGGAWERDEPSGQ
                     YYYHAYLKEQPDLNWRNPEVREAMMDVLRFWLDRGVDGFRVDALRQLVKDEKLRDNPP
                     NPDHRPGQSPYDALLPLYSTDRPEAHGAIRGMRETLAGYGEDRLLIGELYLPIERLVT
                     YYGEDNTGVHLPFNFHLISTPWNARAIAGLIDTYEAALPPGGWPTWVLGNHDRSRIAS
                     RVGAAQARVAAMLLLTLRGTPTIYYGDELGMRDAPIPPELVQDPWEKNVPGIGLGRDP
                     ERTPMQWDGSANAGFCPEDVTPWLPVAEDYEQANVATEREDPVSMLSLHRRLLALRRA
                     EPALSAGSYEPVEADGDLLAYVREREGRRFLVALNLGHEPRSFEHPEIRGRVALTTRL
                     DRAGEEADGALKLRGDEGVIVEIP"
     gene            complement(15491..>16373)
                     /locus_tag="VFJ72_03580"
     CDS             complement(15491..>16373)
                     /locus_tag="VFJ72_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478583.1"
                     /translation="PSRNDEYLLYQTLLGAWPLGEMSEDALADFTGRIKAYMEKAIRE
                     AQVHTSWTKVNEEYEEAVAGFVEAVLSPDNNLFLDDFLTFQRRIARAGALNALSQTLL
                     KLTVPGVPDVYQGNDLWDLSLVDPDNRRPVDYGLRGRLLGDLKAMDPAESNSLLDGDT
                     WKDGRPKLYLTWKALELRRRMPELFESGEYVPLETTGALDDHLVAFARLYEKEAAIMV
                     VPRLYAKLNIGEALMFGEEVWTGTRISLPEELAETPLHNLLTGEKVRIERGEEGAFVH
                     AGGILGGFPVALLAAEG"
BASE COUNT         3069 a         5395 c         5177 g         2732 t
ORIGIN      
        1 agccgcgacc gatccctccg aacgagcggc ctccccgata agatccgggt ccagaccgtt
       61 cgcgcgcatc gcctcggcga tggatacgcc gtccgcgtct tcgtgtcgca aatcgccagc
      121 gtccagctta ccggcgaggg cgcggctccc gatcatgccg aaggagaacc gcgcgccctc
      181 ctcgtccgtg aacgcgacga cctccacggg acgctccgtc tggacgcccc gctcgttcat
      241 cgtatggagg acctcgatac cagccagaac gcccagaggc ccgtcgaagt ctccgccgtt
      301 gtagaccgag tcgacatgag agccgatcag gaccaccgga gcgtccgagt cgcgcccctc
      361 gcgccgcccg aacaggttgc ccgcggcgtc ctcccggacc gagagcccgg cttcctccat
      421 gaaagagatg actctatcct tcgccacgcg ttcctcggcg gtgatagaca gccgggtaac
      481 gccgcccgct tcctggcgac cgatctcgcc gatctccttg atcctgcgcc agatacgctc
      541 accgtcgatc acgatctcac ctccgtgcca gccgcgccac gtactccctt tcctcgtcgc
      601 cgaaccacgg cgggtcaccc gccgccgcgt tttcgctcat gcggtccggg ctgctggtgg
      661 cggggatggc gcagctcacc cgccggtcgg agaggacgta cttgaggaga gcctgcgccc
      721 aggtctcgac gccgaactcg cggagcggct ccaggctctc tgaaggcgga gcgttgcgga
      781 cgagacggcc ggacccgagc gggaccataa cgatgacgcc gagacccagt tcctcggcga
      841 gcggtagcac ctcttcggtg acggaggtat cggcggcgtt gtacgggact tgcacgaagt
      901 ccacccggcc gctccgcatg acgtgcgtga ggtcgccgaa cgccgagcgg gagtagtgcg
      961 tggccccgat ggagcgtacc ctgccctcct ccttgagacg gtggagcgta tccagccgct
     1021 tctcgacggc tacgaggtta tggacctggt agaggtcaac gtacccgccg aaatacccga
     1081 gggcggcctg tatctggccc tccgcctcgc ggtcgtcgga gtgccagacc ttcgtcgcca
     1141 cgatggcgcg ctcccggccg agattccgca gggcgtcgcc caacacccgc tccgcctccc
     1201 cgtacatcgg ggatgagtcg aagaggtccg cgccggcttc cagggcggtt cggacgacct
     1261 cgtggcgagc gtactcctcc ggccctcgca cgtcgagcac ctgccaggtc cccagcccga
     1321 caacgggcgc gtctattccg ctgctcccga ggctgcggcg ttccacgcta acggccttcc
     1381 ggtgcgaagg actcttcggg gcggccttcc agcccggcga cggcggagag gaaccagtcc
     1441 gggcgggact ggaccgcgtt cttcacggga tcgaagaaga cctgggcggc ggagccctcg
     1501 gcggcgaggg tgccggtctc gaaggactct ccggtgcgaa gctcgaagtc catgccgtag
     1561 gagcggttgc cgacggtgcg gatgctggcc gctccgtgga gggtatcgtc caggaagatc
     1621 gggaccttaa atcgcacgct ggtctccgcg acgacgtagc gcgcgccggg gatgtcgccg
     1681 aggacgaggt ccagacccgt cttccgggcc agcgcctgga agtaggccat gcgaagcgtc
     1741 tcgaagaaga cgagatagat ggtgttgtta acgtgcccgt aagggtcgag gtcctggtat
     1801 cggatctcca accgctcgat taccggcggg ttcttcaagc tcctgcccct tcccgctccc
     1861 caaattaaat tccctgctgg ccagagattg tacacattat cggccgttat cttctgattt
     1921 aatttggata tggaatacga ggagtatctg gatcagggcg agaccctcgc ggaggagggg
     1981 cttatcgagg aggctctctc ccgcttcgag caggcgctgg aggccgcccc ggagaacccg
     2041 gaggttatag aggctgtcgg gcgggcgctg ctcaacctgg gacggctgga ggaggccgag
     2101 gcgagctttt ttgacgccct ggagatcgat ccggagtggg ccgcgccgcg catggggctt
     2161 gcgctgctgg cgatgcgccg cgaggagccg ttcaaggtcg tgcatcatct ggagcgcgcc
     2221 atcgaggccg accccgagta cccggacgcc tacgtggagc tcggccgcta ctacggcatg
     2281 atgagcgaac cggcgctcgc caaggccacc ttcgagcgct ggaccctccg ctaccccgaa
     2341 gacgcggaca tgcacataaa cgccggtctc accgccttcg acgccgcgga ctacgcccag
     2401 gctcttgagt tcttcgagaa ggccctggag gtcgctgccg atggagagca gaagagcgga
     2461 gcgcgcacct ttcgggccaa cacgctcgac atgctcggcc gctacgagga ggccctctcc
     2521 gagtacgagc gggtaataaa gtacgccccc gactggtggg aggcccacgc gaacctcggc
     2581 atctgccacg ctcgcaacgg ccacttcgcg gaggccgagg cggcgctgcg cgcgggtctg
     2641 gccgcgtgtc ccggttcgcc cgagatccgg gacgagttgg ccgcgcacct gctcggcgag
     2701 gatggagacc tggaagaggc tctcgctctt gccgaggagg ccgtggccct ggggcgcgat
     2761 gaggtgcgcc acctcttcac gcttggagaa gtccggctcg ccctcgacga cgaggccgga
     2821 gcgcaggaag cctacgaggc ggtgctcggc ctcgaccccg aagaccccgg agcgcacctg
     2881 gagctcgggc tcctgcacga gcgcagggga gaggtcgccg aagccgagga gcacttcatg
     2941 gaggctctca agggagaccc cggcaaccct cgcgccctct actcctacgc cagcctctac
     3001 tacgccaccg acgacctgga gaccgccgag gaactgctcg tgcgggccat cgccgtggac
     3061 gcggagtact cccccgcgct ctcggcgctc gccagcatcc gggcgcgtcg cggcgactat
     3121 ggcatcgccc tcgactacat agagaaggct gtcgaggccg gcgagcgcga cgtggagcac
     3181 ttcaggagcg ccatagagtt cgcccccctg cgcgccgacc cgcgcttccg cggcctgctc
     3241 tcccgcatga cctccggtaa ctgagcgttc atgcccgcca ccctggcctt cgggctcttc
     3301 ctcaccgctg tcgggctcgc ggccctgacc ttcggtctct acgctctgct gcgcggcggc
     3361 aaggagcacc ggggaggcgg gatcggcccg atctccgagc gcggcattca tgtcatcgcc
     3421 ggcatcagga tgaccattat cggggcggcc agcctcgccg ccgggctgta cttcctgtgg
     3481 agttactgga gctagctggc gctccgacac gctccgacgg ctacaggagc ttcagccggt
     3541 ccgtggcgtt gcggaggtct tgcaggaagt cgtccaactc gcggacgcgc tctgccgcga
     3601 tggccttgct ggtctcgaag atgaggaggt ccgggatctg ccgccgcagg ctctcggcgt
     3661 gccagatcgc cgccggtatg tccggcacgt cttcctccac gccgaccatt gccagcacgc
     3721 gcgagacccc gatggccccc aggtaatcca gggcgacggc atcctttaga agagcggcct
     3781 cggtcgtggc gccgggcgga gtccccggag agtgatgctc tatggcgtcc aggaccacgc
     3841 gggcggcctg cggcgggtag tccaggtcct tcaggatgcg gccggcgacg gcggaggagc
     3901 gcttcgcgtg gtccgaagcc tcccgcagcg cgtaggcctt gtacagaccg acgtcgtgca
     3961 ggagggccgc gatacgcagg atctccgagt catacgaggc gtgctcggag gccccgatct
     4021 cctcggccag cgcgtacacc cgcaggcagt gcttgtatcc ccataccggg tgcgccccca
     4081 cgcgggcaac gatgtcctca acctcatcaa ccagcagcct catagcagga agagtctagc
     4141 ggatgcggct taactgcgcg actacgtcaa aagggttagg ttctttgagc cagataaagt
     4201 tacctcatct gcgcgatgcc gcgatccggg ctggggcata acctccaacc gcataccaat
     4261 accgccagac gaatgtcccg cgcacactcc gggaagtaaa aatgaagcta acccgacgag
     4321 agatgctgaa gttaggtctg ctgggcagcg ccgctctaat gctccccgtg gagcgtcagg
     4381 ccaggacgga cctctcgata aagaaccgca tcccagagag ccaactcccc cggccgttcc
     4441 aggtcccctt cgccgtgccg ccggtcgccc ggcccgtgaa gcggaccgcc acgaccgacg
     4501 tctatcagat gagcatgcgg gcggtccagg cgcagatact gccgggcttc ccaaagacca
     4561 cggtcttcgg ctacgacggc ctcgttccag gcccgacgat cctggccctc gccggaagac
     4621 cggtcctgat ccgccacatc aaccggctcc cggccctctc gcctttcaac accccgacca
     4681 cgacctcggt ccatcttcac ggctcgccgt ctctgccgca gtacgacggc tacgcgagcg
     4741 acgtgaccgc gccgggccag tacaaggact accgctatcc caacgtgctc aacagcgcct
     4801 gcacgttctg gtaccacgac catgccgtgc accacactgc cgagaacgcc tacatgggac
     4861 tcgccgccca gtaccatctc cacagcgacg ccgagctggc cctgcccata ccgcacggac
     4921 gctacgacgt gccgctcacc ataaaggacg ccatcttcac cacgaacggc tcgctcgtct
     4981 tcgacgacaa cgaccattcg agcctcttcg gcgacgtgat cctggtgaac ggacgtccgt
     5041 ggccggtgat gaaggtagag cgccgcaagt accgtttccg ggtgctcaac gcctcactct
     5101 cccgttctta caggctcgcg ctcgactccg gagagccctt taccataatc ggcaccgatg
     5161 acggtctcat gcccgcgccg cagagagttc agaccttccg cctggccatg gccgagagat
     5221 acgaagtcgt catagacttc gcccggcaca agatcgagga caggataatc ctgcgcaacc
     5281 tgagcaaccc caacaacgag gactatccct cgaccggacg ggtcatgatg ttcgaggtcg
     5341 cgagtaacgc cacggacacg aagaacaaca ccgtgcccgc cgtcctgaac cctgacaacg
     5401 ccaccatgaa ccttaacgag actcaggccg tgcggacccg gcggatggcg ttcgagcgcg
     5461 atggcggcca gtggaccata aacggcagca cctgggacga cgtaataaag agcggcttcg
     5521 agttcgtgat cgccaacccc ggcctgggcg acgtcgagat ctgggaggtg tccaacgact
     5581 ccggggggtg gttccatccg gtacatatcc accttataga tttcagggta ctggaccgta
     5641 atggcaagcc tccgttcccg tacgagatcg gccccaagga cactgtctac gtcggcgagg
     5701 acgagaaggt gcgcctgatc atgaagttcc gcccaaacag aggcaggtac atgatgcact
     5761 gccacaacct cgtccatgag gaccacgaca tgatgaccca gttcgaggtt ggacagggag
     5821 gcaacgatcc cttcagcgcc ccggccagag acctgccggc tccctccctc taggaaacct
     5881 ctgaagaacc cgccgcgtcc gggtccggca cggataaacg cgccgcctcc accagtgctg
     5941 gcgtatccgg gagagcctcc gggtggagga tgtggcccag tacctcgaca cctcttacca
     6001 gccggggcgc cggacgggag aagtacgagt tcgcatccac cacccacacc cggccgtcct
     6061 ccacggccgg caggtccttc cagcctggta gatccggcag gacccgcgcc tcccgcgcgg
     6121 cccgctccgc gccgaagccg cagggcatca ggacaaggac ctccggggcg gcctcgaaga
     6181 cctgttccca ggtgatccgg gcggaagcct cacccggcgc ggcgagcact tcctcgccac
     6241 ccgccaaacg gaccatctcc gggacccagt gcccggccga gaatagcgga tctagccact
     6301 cgatgcaccc gacgcgcgga cgcggcagcc cggcaaccgt ctcctccacc cgcgccagcc
     6361 gctccctcag cgcggccacg ttatcatgaa cctcgttacc ccggccgaga gcctccccga
     6421 cacgcgccgc gtcttcgagg acgtcgtcca gggaggtcgg gttgagggag agtatctccg
     6481 gcctgttctc cataccggcg accgctcgct cgacgaggtc catcgagacc gcgcacacct
     6541 cgcagagacc ctgggttatg agtagatccg gggtcagatc ttccagaagc ttcgcgtcga
     6601 gggcgtagat gctgctgccg tcggtgagta ttcggccgat ggccgcgtct atctcggcgc
     6661 tggtcattct cccggcgtgt atcttgctgc tggtgagcct gggcaggtcc tccacgccgg
     6721 gcggatagtc gcactcgtgg gtgacgccga tcagggtatc gcccgcgccg gcgaagtgga
     6781 ccatctcggt ggcgctgggg aggagtgatg cgactcgcaa gctatcgctc cgctagtact
     6841 tctggtttcg gacgtcgcgc aggagagcgg cctgtccgat gtcgtctatg gtcagtaagc
     6901 cgacgaggtc accgtcctgg acgacgggga tggcgcgcag gccggactcc tgcaggagac
     6961 ggttgccatc cttgaagagg tcggcctccg gagagatggt cgggaaatcg gtcttcatca
     7021 ggtcgcggac gttcaggaac ttctgcgggg agtgggcggc ggacatgatc tcgctgcgcg
     7081 taatgaggcc gacgagccgt ccgtcctcgt ccaccaccgg gaagtcctcc tggtagccgt
     7141 ggattaccat gtcgaagacc tgtccgaagg tatggtacgg cgtaaccgtc tccgtgcgcc
     7201 ggcgggtccc catcacgtcc gagacggcga gcccgcgcat cgtctcgcgc tggcggacca
     7261 tctgcgcctc gccgctcgcc ccgaagaaga tgaagacgga gataagcatt agtatgacgc
     7321 cgccggtgaa aagcccgacc ataaagaacc cgaccgcgaa gagctgcccg acggtcgagg
     7381 cgatatccgt cgcccgcacg ggtccgaggc gggtcgccag gagcccgcgc aggacgcgcc
     7441 cgccgtccat cgggaaggcc gggatcatgt tgaacaccac cagcgcgacg ttgacgatcc
     7501 cgaggtatgc gaagacctgt ccgagcgagg cggtcccctt gagcacgttg gcggggttga
     7561 agaggtccgc gccgagcgcg aacgcgacga agaagaagac cggggcgagc accacgttca
     7621 ccaggggtcc ggcgatggcg atcttcacct cgtcccaggg cttttcggga aggttcttga
     7681 gccgggccaa tccgcctatc gggagaaggg taatgtccgg gacctcgata ccaagccgct
     7741 gggcgacgac ggagtgcccg aactcgtgca gcagcacgca gaagaacaac gccactatga
     7801 tcccggaggt ctccagagcg cccagaacgg tacccgcccc ctgatatccc aggtaggcga
     7861 agaaagccag caacaggaag aacgtccagt ggacctttac ctcaatgccg ataacccggc
     7921 cgatcttgaa cgaaccgccc atatacagaa gtttaaccca cggacaggaa cagcacgtga
     7981 tccctcttcc accccatcac gcctgttaga atcgaaccgt ggaggaacgt accaggaact
     8041 accaactcac taccgaagcg gaacaactgc cgcggatcgc cgagaccctg aaagaagcgg
     8101 aggctatcgg cctcgacacc gagaccaccg gcctgagtcc ccgcgacggc cggacacgtc
     8161 tgctacagct agccaccccc gaggagacat acgtcataga cgtctttgag gtgggcgacc
     8221 tctcgccctt gaaagacgct ctcgaaggcg gtccggtcaa ggtcttacac aacgcgaagt
     8281 tcgatcacgc ctttatgtgg gccgagcatg acatctccct ctcccccatc ttcgatacga
     8341 tgctcgccgc gcaactcctc tccggtggag actatgacaa gtcctactcg ctggagagcg
     8401 tggctcagaa gtacctggac gcggagttgg acaagtccgg tcagcgcagc gactggtccg
     8461 gcgctctgtc ggatcgccag atcgagtacg ccgcccgcga cgccgaggta ttactgccgc
     8521 tacgcgaacg gctggccgcg gcgctcgccg aagataagct cggcacggtc tcgaagatag
     8581 agttcggggc ggtggcggcg atctcggaga tggaactcgc ggggatcaag ctagacgtgg
     8641 cgaagtggaa ggagctggag atcaccgtcc gcgagcggcg cgatagagcg gcagagcacc
     8701 tcgaatcttt cttcccggag ccggaaggcg tgctgccgct ggaggggcta ggcccgcgtc
     8761 tcaacctgaa cagcccgcaa cagatcacgg acgccttccg ctctctgggc atcgacctcc
     8821 cggatacgaa ggtctggacc ctcttgaagg tggaccaccc ggccgcgaaa gccctgctcg
     8881 aataccggga gctgcagaag aagctcggca cgtacctgga gacctatcct aagttcatcc
     8941 acgagaagac cggacgcatc cacgccaact tcctgcaatg ccgggtccca acaggacggc
     9001 tcgcctgcac aaacccgaac gtgcagcaga tcccacacga ggacgagttc cgcagttgct
     9061 ttgtcgcaga acaaggtaac gttctcgtca tcgccgacta ctctcagata gagcttcgca
     9121 tactcgcgga ggtctccgaa gacccggcat tcgtaacggc cttccagaac ggcgacgacc
     9181 tgcaccgggt aacggcggcg acgatgtacg gggtgccgat ggaggaggtc acgaaagacc
     9241 agcgttcgga cgccaagcgc ataaacttcg gcctaatgta cggaaggggc gcgaagagcc
     9301 tctccgccca gctcggcgcc agcgaagagc gcggacggca gctcatagaa gagtacttcg
     9361 ccaactaccc gaaggtgcag cgcttcctgc aagagacggc gagcaaggcg acgaagaccc
     9421 gtcggctgcg aaccctctcc gggcgtttga ggaagttcca agacgcgccg gtctccgacg
     9481 accggggggc gctccggcgc gaggcgatga acttccctat acagggagcg agcgccgaca
     9541 tagccaagct cgccctgatc tacatccgca aagagctaaa agacctcgac gcccggctca
     9601 taaactccat ccacgacgag ttcgtcatcg agtgcgccga ggagttggcc gaagaagtct
     9661 ccgagcggac cagatcggcc atgatccggg ccggcgaagt cctcctcaag aaagtcccgg
     9721 tcgaggtcga ggtgggcgtt tcgcgcgagt ggcggaagta gtggaggttc gtataggtcc
     9781 ttgtaattag cccggacgat ctgtgcctcc gtccccattc cttcgcctcc ggacgatatg
     9841 gagacacatt ctatagttag cggctccgac gctggcagcg ccccgaacca cagtgcatac
     9901 gcgctctatt caacagagcg tagctgatcg cggtcctgaa cgctttgctt ctcagccttt
     9961 cccggggacc gggcccggat tcgggtgctc tggcaggatc attggcgacc gaggtttctg
    10021 gttgaagtcg aaatctttta ccaggttccc catttgaggc gcgttctcac gcacgtcggg
    10081 gcgtggatcc ggcctgccat ccgtctgagg atcgaggcgc tggccgttca ggaagtcgtc
    10141 ctcgatgaac ttcaggtagg catcgaagga gagtgtctga tggtctatgt atccctgtct
    10201 ggcatagggg cttattacca tcgccggcac ccttatcccg tagccgttct cgtccactac
    10261 cggaggcttt acgtggtcgt agaagcctcc ccagtcgtcc caggctaaga agacggcggt
    10321 gctgctccag tcggggcttt tcatggcggc gtttatgagg ccggtgacgt aggactggcc
    10381 cttgctcacg agagccgggg ggtgttcact gttcttgccg tcaggcacta tccaggagac
    10441 cgctggtagg ttccccttct tagcgtctgt gtagaagttc gagacgttct ggatgttgcc
    10501 ggtctggcca ttccctttga cggtatcaaa gtaagggagc ggattccaaa tccccggcgt
    10561 tctagaactc tggttcaggt gttcacagaa catctcgtcg ttgcggcagt caggctcctt
    10621 ccccctggat acgaagtagc cccaactcac gttgttctta tgcaggagat aggtgagatc
    10681 cgtccaggcg tagtccggcg ggtggacctg gctcctgccc tgcctggcct tcttgctgga
    10741 gtcaggagga ggttcgggtc tctgcagcgc gttccggcaa ctcatcgggt cgccgggtac
    10801 gctacacgtc gccgaccagc cggagaccat gtaaaggtgc gccggcaggc tccaggagga
    10861 gttggactcg aacatgtggt cctgcaagac gaagtggcgc gcgtaggacc agtagttggg
    10921 tatctccctt ccgtcgtggt agcccataac gtccggaggc gtgtctttag aggactgtcc
    10981 gcaggcgggg tcgaagcggt tctggcagga cctcttgcga ccggcgatct cctgacgaat
    11041 aaacccatcc atcttccccc cgttaatatc ctgactggcg tccgccgccc ggtgtggggc
    11101 cccgtagttc aggtctttcg ggtcatgaaa aggtttgaca caccggctcg cttgcggatc
    11161 ggggacgcag accgtcggtg tgccgttctt catgggtata ccgtcggcgc cgggaaaagt
    11221 tccgaagtag gagtcaaagg agcggttctc ttgcatgatg atcacgatgt gctttatctt
    11281 gtggatattg gatgccgctg gcgcgacggt cgttgaactc gggaccgtcc gcatctgaac
    11341 agcgccgcct ccacaagccg ccgaaagcgc tacgagacat aagatcagaa gggtaacaac
    11401 gagtctacgc cgggggtctc tcgcacagta catcgctgca ctcctatcta ctatgcagcc
    11461 aggagatcga aggtttgcag tgccgccacg gcattatgaa aggtgctccc gaaatcggag
    11521 cccgcgccct tctgacggga gcgtgccttc tactgcacct tgccatggaa accatttgcc
    11581 gtaagtatct tcgcctttcc taagaaaata ctaagagcct cctcaagcgg tgagaacttc
    11641 tcaagcatcc tgaccgtatg ctccgaaccg agatgccccg gcggcggcca cggtgacgaa
    11701 taggatcgcc agtatcgtct tcagtcccag ccgctcgcca aggacgataa agccgacgag
    11761 ggcggcgacg gcgggctcca ggctcatcag gacgccgaag acgcgggcgg ggactttgcg
    11821 gagggctttc atctccagcg agtaggggac ggcggctgag agcatagcaa cgccgaagcc
    11881 ggcgatgagc agcctcgggt cgagcagagc gtatccgccg ccggctatgc cgaccggcag
    11941 gaggatgact gttcctatgc acagcgcgat cacgagtccc gtcccaccgg ggaacacgct
    12001 ccccgtccgg gcgctgagta agatgtagga ggcccagaag aggcccgcga gcagcgcgaa
    12061 cgccacacct accgggtcga gggccgagga gccaagtacg ccgagcggcg cgagcagcag
    12121 tatcccggcc gccgcgagga tgacccagag gagatccagc agccgccggg aaccagcgac
    12181 cgcgacgccc agaggtccgg cgaactcaag ggttacggcg acgccgagcg ggatgcggtc
    12241 gatggagagg tagagcgaga agttcatcgc cgccagcgtg agaccgaaga gcagcgcgat
    12301 caggtactcc cggcgcgtat atccccgaat acgggggcgc cagatcaggc ccagcacgag
    12361 ggcggcgaat gagatcctca ggaacaccgt acctccgggg ccgaggtctt cgaagagccc
    12421 cttggcgata gccgctccga gctggaccga gaggatagcg agcagcacga gactcgtggg
    12481 cggcaccgcc cccgcgacgt cccggatacg tccgacgtat gaatgttcgc ccgtctttac
    12541 ctccagacct tctgtagctt gcggcaaagc gcggagagtc tagcaccgtg agagaagtgg
    12601 cggagtatct agcctttcgc cgccgcttcg gggactggcc ggcgagtgcc gatgaatagc
    12661 acggcgaggg ccgtggcgga gcagatcgcg cccgtccatc ccagggttgc gagggtgccg
    12721 tggccgagga ccagagagcc gatgctcgcg ccaacgccct ggccgaggta gatggcggag
    12781 gcgttcagcg agagtgccac gttgctctgg gtgggggcga gctgtatcac gcggtactgc
    12841 tggagagggt tgatcgcgaa cccggcgatc ccccaggccg cgagtaccgc cacgactccc
    12901 gcaatagcca gcgccgagcc cgccaccacg aagagtcccg agagagcgag gagcgagaac
    12961 gagaggacga tgaggatggc cgccatgagc cgcccgtatc ccgcgttgtc cgcgccgtat
    13021 cctcccaggc tgttgccgaa caacgccgcc accccgaaca ggaacaggag cccgctcacg
    13081 cccccgcccc cgaagcccgt cagggtgccg agcagcgggc taatgtaggt gaatgccacg
    13141 aacccgccca tcatcccgaa cgcggtcagc cccagagcgg cgactatggc gggatgcttc
    13201 aggactccca cccgctcccg caggctcacg ggtgggggcc cctcgacatc ggggagcagc
    13261 gcccggatac cgagcaccgc ggcaagaccc atgaccccga ccatcacgaa ggtcgtccgc
    13321 cagccagcgt acgtacctat aaccgatccg agcggtatcc cgaccacgaa cgataccgtg
    13381 aggccgcccg tgacgataga gagcgccttc ccccgtcctc cgggcggcgc gatactcgcc
    13441 gctacagccg ccgccgtcgg cgtgaaagcc gccgctccgc ccgcggccag caccctgcac
    13501 aggagtagca actcgaacgt cggggcgacc accgccagcg cgttggccac cgagaacagc
    13561 gtcagggaga cgattagtag cctgcgccgc gacactgctc ccgtaaaagt ggcgagcacc
    13621 ggagagctga ccgcatacgt aactgcgaag accgtcacca ggtgtcccgc agccccgacc
    13681 gagaccgaga ggtcctccgc caccccttcc agcaaccccg cgaagacgaa gcttcccgtc
    13741 ccgatggcga aggtcccgag agccagcacg aaaagtcgta tgttcatctc taccagctct
    13801 ctctggtcct ggttatccgg ctacgggacg gtaacacgaa ccgggccgaa aacaaagcgc
    13861 tgacccgcgc atcctccacc ggacaaccgg tatcagggga tctcgacgat cacgccctca
    13921 tcaccgcgca gcttcagcgc gccgtccgct tcctcgcccg cgcgatccag tcgagtcgtg
    13981 agcgctacgc ggccccggat ctccgggtgc tcgaagctcc ggggttcgtg acccaggttg
    14041 agggcgacaa ggaagcgccg tccttctctc tcgcgcacgt aggcgagcag atccccgtcc
    14101 gcctctaccg gctcgtagga accggccgag agcgcgggct cggcgcggcg cagggcgagg
    14161 aggcgtcggt gaagcgagag catggagacg gggtcttcgc gttcggtggc cacgttggcc
    14221 tgctcgtagt cttccgcgac ggggagccag ggcgtaacat cctccgggca gaagcccgcg
    14281 ttggcgctcc catcccactg catcggggtg cgttccgggt cgcgtcccag gccgataccg
    14341 gggacgttct tctcccacgg gtcctggacg agttcgggag ggatgggcgc gtctcgcatc
    14401 ccaagctcgt ctccgtagta gatggttggc gtgccgcgca gcgttagcag gagcatggcc
    14461 gcgacacggg cctgcgctgc cccgacccgg ctggcgatgc gggagcggtc gtggtttccg
    14521 agtacccagg tgggccagcc gccgggaggg agcgcggcct cgtaagtgtc tatcaggccg
    14581 gcgatggcgc gagcgttcca gggcgtggag atgaggtgga agttgaatgg gagatggacg
    14641 ccggtgttgt cctcgccgta gtaggtcacg aggcgttcta taggcaggta cagctccccg
    14701 atcaggagcc ggtcctctcc gtacccggct agagtctctc gcatcccgcg gatcgccccg
    14761 tgcgcctcgg gccgatcggt cgagtagagc gggagcagcg catcgtatgg actctgtccg
    14821 ggacggtgat cggggttcgg cgggttgtcg cggagcttct catccttcac gagctggcgc
    14881 agggcgtcca cccggaagcc gtccacaccc cgatccagcc agaagcggag cacgtccatc
    14941 atcgcctccc ggacctccgg gttgcgccag ttcaggtccg gctgctcctt gaggtaggcg
    15001 tggtagtaat actgccccga cggttcgtcc cgctcccacg ccccgccgcc gaagctggcg
    15061 acccagttgt tcggcgggcc tccgtccggc gcagggtccc gccagatgta ccagtcgcgc
    15121 ttcgggttct cgcgcgagga gcgggactcc acgaaccagg gatgctcgct ggaggtgtgg
    15181 ttcgggacgt agtccaggat gaccttgagg ccgagacgat gagcttcagc gacaagagcg
    15241 tcgaagtctt ccagggtgcc gaacatcggg tggacgccgg tgtagtcgga gatatcgtag
    15301 ccgaaatcgg ccatcgggga agggtagaac ggggagatcc aaacggcgtc aaccccgagc
    15361 cactgcaagt attccagccg ctgtgtgatg cccggcaagt cccccactcc gtcgccatcg
    15421 tagtcctgaa aggagcgagg gtagacctga tagatcacac cccgctccca ccaccgatgg
    15481 ccctcttcca tcaaccctcc gccgccaaca gagctaccgg aaagccgccg aggatccccc
    15541 cggcgtgtac gaacgcgcct tcctctcccc gttcgatcct cactttctcg ccggtaagca
    15601 ggttgtgaag cggcgtctcc gcaagctcct ccggcagcga tatgcgagtg ccggtccaca
    15661 cttcctctcc gaacatcaga gcctcgccga tgttcagctt tgcgtacagc cggggaacga
    15721 ccattatggc ggcctctttc tcgtacagcc gcgcgaaggc gacgaggtgg tcgtccaacg
    15781 ctcccgtcgt ttccagcggc acgtactcac cgctctcgaa gagttccggc atccgccgac
    15841 gcaactccag cgccttccac gtcaggtata gcttcggacg tccgtccttc caggtgtcac
    15901 cgtcgagaag cgaattcgac tccgccgggt ccatagcctt gagatcgccg agcagcctcc
    15961 cgcgcagtcc gtaatcaacg gggcggcggt tgtccgggtc aacgaggctc aggtcccaca
    16021 ggtcgttgcc ctgatagacg tccgggacgc cggggaccgt gagcttgagc agggtctgcg
    16081 agagagcgtt cagggcgccc gcgcgggcta tcctccgctg gaacgtcagg aagtcgtcca
    16141 gaaagaggtt gttatccggt gagaggaccg cctcgacaaa tccggctaca gcttcttcgt
    16201 actcctcgtt caccttcgtc caggaggtat gtacctgcgc ctctctaatg gccttctcca
    16261 tgtacgcctt tatgcgcccc gtgaagtccg cgagggcgtc ttcgctcatt tctcccaggg
    16321 gccacgcgcc gaggagggtc tggtacagga ggtactcgtc gttgcggctg ggg
//
LOCUS       DASSMB010000052        12784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_266138, whole genome shotgun sequence.
ACCESSION   DASSMB010000052 DASSMB010000000
VERSION     DASSMB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266138"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..822
                     /locus_tag="VFJ72_03585"
     CDS             136..822
                     /locus_tag="VFJ72_03585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7478584.1"
                     /translation="MPEIDAMSIIEAVFPELSGRHILDVGCGKGALARALSVRGVRVV
                     GVDPNGEALKAARQTAPTAEFYMAGAEALPFADRTFDGVIFLNSLHHVPESVMRRALL
                     EAARVVISVGPVIVIEPLAEGSLFSVLRLVEDETGVRAAAQEAVREALDDGTFGQLNR
                     IEYLRYDRYTDVDELLSHVISVNPSRAEVARERRAEVEAAFLRCAQAAADGRMTLEQP
                     MRAHVMAVTV"
     gene            complement(824..1669)
                     /locus_tag="VFJ72_03590"
     CDS             complement(824..1669)
                     /locus_tag="VFJ72_03590"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HET7478585.1"
                     /translation="MRYVRPPRISRLLPVILGAVQPQPGERQRITNRSLKLVLDIVLG
                     AVVPILVLSYLSDPLGAVPAYVVSALVPVGWVLIDLLFVTRRFNFITGFLGLSAIVRG
                     LLAFWFVDGTLFALKDSVGGVLTALVFGGSALVGRPLVGAFVAQGLDPQTPEQEASLV
                     RLLAERPVARALVASTAMISLLNAATSVANFLLNLCIVTASFGTGEFNSQVARVNAIT
                     RVALGIPEFLAMGFAIWGVIYVLHSRLPEDTKEKDFWDLVETSEEQARHPEDPGNTST
                     RRPSG"
     gene            1881..2315
                     /locus_tag="VFJ72_03595"
     CDS             1881..2315
                     /locus_tag="VFJ72_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7478586.1"
                     /translation="MKAPTILLVEDSPDDELLTMRALRKNGMGEAVAVARDGAEAIEY
                     LRNARSLPGLVLLDAKLPGMSGMEVLRIIRADERMKSLFVAVLTSHDEGGLMTGDQYL
                     RADLFIRKGVNYDQFSDEIRALKPLLTEVIARRNSPCAGPIS"
     gene            2452..2850
                     /locus_tag="VFJ72_03600"
     CDS             2452..2850
                     /locus_tag="VFJ72_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478587.1"
                     /translation="MSNENRDLVRRAFEACQQADTTTLKQCFSYDRSVQIQGLARPNP
                     YPAGSNVDLHYDTPVEEANSKVRTSYRLKTDDGSEYHAEVVDTIENKKISSSVVTWTD
                     WDPSGNPEDDAAWYEGKYGWWGWPFGRGWW"
     gene            2908..5115
                     /locus_tag="VFJ72_03605"
     CDS             2908..5115
                     /locus_tag="VFJ72_03605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7478588.1"
                     /translation="MNLRHHAINRRGRGWRRALAPGRDSAVNANEPLPALRGHRLAAA
                     RTIWVVVVLAALAVFAAGLPAYYDQIVQAAAANTSRGAGLRDNLGSLGLSVGFYAGWF
                     GVVIPVVMAAAFSLVAAVIFWRRSDEPMALFVSLLLALFGATRSDANEALAQVHSGLG
                     PLSSALESLSVVSVFAFLFVFPNGRFVPRWTRWLVVPAAAYSLLTSVVPDSSLDPKHW
                     SGLPYTAFFGVWFLGGAVAQVYRYRRVSGPVERQQTKWVFAGFTAALAAYLGMILLFF
                     IVLTPPEPGTLPDFVLGTVFYCSTLFIPISIGVAILRYRLWDIDVIINRALVYGALTA
                     IILALYALVVGGLGLFLQGRGNTLASLLAVGLVAVIFAPLRNRLQRAVNRLMYGERDD
                     PYAVLSRLGRRLDAALEPGMVLPTIVETVAAALRLPYAAIELKSQDGFELAAAHGDPT
                     DEQTFLPLSYGGETVGRLVLAPRAPGEAFSPADRSLLEDLARNAEVAVHAVRLTADLQ
                     RSRERLVSAREEERRRLRRDLHDGLGPTLASLGLGIDVTLKILEENPEEAEAILRQLK
                     SQTKDAVVDIRRLVYDLRPPALDDLGLVAAIREQAANQGILASELTSGGARRNSGLVF
                     EIEAPAELPPLSAAVEVACYRIVQEAITNIVRHARASNCRVRITVDKSIRMLEIEVSD
                     DGIGMPEDRRAGVGLSSMSERAEELGGRLLVEPAPGGGTRVLARLPLLTKEDG"
     gene            5117..5791
                     /locus_tag="VFJ72_03610"
     CDS             5117..5791
                     /locus_tag="VFJ72_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7478589.1"
                     /translation="MADRRDPSEKTRVLIADDHALFRYGLKAMLRSEPGFEVAGEAET
                     GEKAVDLTRELKPDVVLMDIQMPGINGIEATRRILESDPDTGIVVVTMFGEDDSVFAA
                     MRAGARGYVLKGADAEEVLKVLRAVADGEAHFGPDIARRLRGFFSAPRPRPEEAFPEL
                     TAREREVLDLIASGLPNAEIATRLYLSEKTVRNNVSAIFLKLRVADRAQAIVRAREAG
                     MGRGNA"
     gene            complement(5848..6198)
                     /locus_tag="VFJ72_03615"
     CDS             complement(5848..6198)
                     /locus_tag="VFJ72_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478590.1"
                     /translation="MLLATEQSFHREAVATALRPLHPSVEVESTEPACIENAASRLAP
                     DLVICSQTTPAARSGGVAWIQLSPNGYWLASIRGADDPDRRLFVSGTGLSDLVSTVDF
                     LSGTLADQQFATST"
     gene            complement(6366..7145)
                     /locus_tag="VFJ72_03620"
     CDS             complement(6366..7145)
                     /locus_tag="VFJ72_03620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7478591.1"
                     /translation="MRDFAQRHPLLFALSILAALVGLTLVARTLLPSAPIGDVSGLSP
                     EALKPPSPLQQAITAIKNADNLFWVLALALAALLLTALGWWREAGFRAPRWRKLYLLV
                     FPLAVCAFALSDGFLLPDKGPLVAGLLVVFIAAIGEEAVFRGLLWRVFVPRGPVWAVV
                     VTSLLTGALYLARSTNDALPEAIRLSAVAACGGFTYGALRWRTASIWPVVGVHAALAY
                     VPTVAALGSTKYPILVWISTLGFVLYGLLLLRNRRVRADGR"
     gene            complement(7150..7776)
                     /locus_tag="VFJ72_03625"
     CDS             complement(7150..7776)
                     /locus_tag="VFJ72_03625"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET7478592.1"
                     /translation="MVVEKRQPLSRRRVLEAAVRYVDREGLEALSMRKLGAELGVEAM
                     SLYNHVPNKEALLDGMVEVLLGELEVPAKSKDWEERVRGAYRAFRRLAHEHPNIFPLL
                     ITRPPDTMYGAWLVEEFLETLHGAGFDAETGLHAYRVLSSYAAGYAMAEIRDFSLQSG
                     AAPPGSANLSTEEFPRLAEISPKVRDMDLDAEFELGLDLILSGLKARL"
     gene            complement(8056..8337)
                     /locus_tag="VFJ72_03630"
     CDS             complement(8056..8337)
                     /locus_tag="VFJ72_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478593.1"
                     /translation="MEAPITQATGHNNTIVSVYPDRVELRTGWQGQNVERIGLRDIAA
                     VGTSGVVNVTLNLQTNTGQVYKLTRMALPDARRIKTAIEAQKQRAGLYE"
     gene            complement(8474..9793)
                     /locus_tag="VFJ72_03635"
     CDS             complement(8474..9793)
                     /locus_tag="VFJ72_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET7478594.1"
                     /translation="MIVAAVLVLFMQAGFAMLEVGFSRMKNVGSVVAKILVNLAIAAL
                     LFWAVGFAFTFSDGGALNSIIGTQGFFLNVPEGQVDKMITGLSWTQVPLSAKFLFQVA
                     FCAVSLAIVWGTMLERTKFAVYVVFAVVFAGLIYPLIGHWIWGGGWLASLGMQDFAGS
                     TVVHLSGAMAALAGTLLLGPRLGKYDDAGRPVTIPGHNMPLAVLGVLILWIGWYGFNP
                     GSTMAATPQIADIALTTTLAAAAGVLGAMLMSYTYRRHVDVGMGGNGAIAALVAITAP
                     CAFVAPWASIIIGFMAGVIMYATLVYVDKIGVDDPLGAIAAHGMGGIWGTLSTGLFTT
                     PALAAVGKPGLFYGGGFTQLGVQALGIVACGGFVFLSSLAVFAALKATIGLRVKPEQE
                     LNGLDISEHGVFGYPDLVATDYQNGNGHKTTTDMVEAKPKGVTEGTI"
     gene            complement(10109..11872)
                     /locus_tag="VFJ72_03640"
     CDS             complement(10109..11872)
                     /locus_tag="VFJ72_03640"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7478595.1"
                     /translation="MTGFAIRFASAHNDTDWLHYVANGDYLLLKDFARILLEELGADC
                     PLPGDVSRTVRGVAVEEPIKGWLEPDEAAVTARDSLDEDFLRTVSGAGAPAVVWRSET
                     APDSETMELTAALGLGLFVLQPEVPLRRLLSLFSEGDGLLLHSHGAVRTLLESTGGDT
                     SIEGLTLRISELLKREIVVEDPVGRLLAWSQDATSGLHETIEEHGLRASRSAGVDETR
                     RERRDRYARLPDGYLSVPVERWRIEDEELFWTPIGTGAPAGYMWIDLKDKPLGPRDVV
                     LLYWARRILESEMGKERIRLETELGVRGDFVDDLVNGHYGSVELLLQRARYLGADLAD
                     GALVLIVDIDHFARYLERRKLKEPAIQELKKRLADAVRLQTRQLFTNFLLGPRSDNVI
                     LFLGGSEDSGATQIPENARRLAAQIQRYVKGLLPDLTVSIGIGRYKDDPISLPDAYSE
                     AEVALEIGHRIHGPSSVSTFEGTGTYKLLFRVLQEDPAELESFYSETLEPVVLYDSRY
                     GTELVQTLLTYLGNDASTVKTASDLFAHRHTIRYRLDRVGELTGLDVDRSEDRERLTL
                     GIKAMQLLGRVPEQPSSLSDR"
     gene            12008..>12784
                     /locus_tag="VFJ72_03645"
     CDS             12008..>12784
                     /locus_tag="VFJ72_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7478596.1"
                     /translation="MRGAREHNLKDVTVSLPRDVMVVVTGLSGSGKSSLAFDTIYAEG
                     QRRYVESLSAYARQFLGQMDKPDVDHIDGLSPAVSIDQKTTSNNPRSTVATVTEIYDY
                     LRLLYARAGRPHCHVCGFPVASSTPQQMVEKVLTLPEKTRFMVLAPVVRGRKGEYGKM
                     FKDLAEDGYARVRVDGELHELPVDLNLDRKYKHDIEVVVDRLVIREGMERRLTDSIET
                     ALKLAEGLVQIETVETDGPGESMLFSESFTCTNCGASIAEI"
BASE COUNT         2350 a         3913 c         4298 g         2223 t
ORIGIN      
        1 cgaatcaatc caccaactca gagatgagtt gacggtcttc aagaaagagc atccggattt
       61 accctggaat ggtggtgact ctggaaccaa tcagtagggt tctgcaagcg gcgatctctc
      121 acagaggata attaattgcc agagattgat gccatgtcta ttatcgaagc ggtgtttcca
      181 gaactctcag ggaggcacat tctcgatgtc ggttgcggca agggtgctct tgcgcgtgcc
      241 ttgtcggtgc ggggggtgcg ggtcgttggg gtggatccaa acggcgaagc tctgaaggcc
      301 gcgcgtcaga ccgcgccgac ggcggagttt tatatggccg gcgctgaagc gctgccgttc
      361 gcggatcgca cctttgatgg tgtgatcttc ctgaactccc tgcatcacgt gccggaatcc
      421 gttatgcgcc gggccttgct ggaggcagcg cgcgtcgtca tttctgtggg tcctgtaatc
      481 gtcattgagc cgcttgctga aggctctctg ttctccgtgt tgcggttggt ggaggatgag
      541 acgggggttc gcgccgccgc gcaggaggcc gtcagggaag ctctggatga cggaacgttc
      601 gggcagctca accgcatcga atatctacgg tatgatcgat acacggacgt agacgagtta
      661 ctgagccatg tcatctccgt gaacccttcg cgtgctgaag ttgccaggga acgccgggcc
      721 gaggtcgaag ccgctttcct gcgctgcgcg caggcggccg cggatggacg aatgactctg
      781 gagcagccga tgcgcgcgca cgtcatggcc gtgacggtgt gactcacccg gaagggcggc
      841 gcgtagacgt gttgccaggg tcttccgggt gtcgtgcctg ctcttcgctc gtctcgacga
      901 gatcccagaa gtccttctct ttcgtgtctt ccggcagccg ggagtgcagg acgtagatta
      961 ccccccagat cgcgaacccc atcgccagga actccgggat gccgagggcc acgcgggtta
     1021 tggcgttgac ccgcgccacc tgggagttga actcgcccgt gccgaaggaa gcggtgacta
     1081 tacagaggtt gagcaggaag ttggcgacgc tggttgcggc gtttaacagg gagatcatgg
     1141 cggtgctcgc tacgagagcc cgcgctaccg ggcgctcggc gaggagacga accagcgagg
     1201 cttcctgctc tggggtttga ggatctagac cctgggctac gaaggcgccg accaggggac
     1261 gcccgaccag cgcagagccg ccgaagacga gggccgtcag gacgccgccg acgctatcct
     1321 tgagggcgaa gagcgtcccg tccacgaacc agaatgccag gagaccgcgt acgatcgcgc
     1381 tgagtcccag gaagccggtg atgaagttga agcgccgggt gacgaacagc aggtctatga
     1441 gcacccatcc gacgggaacg agagccgaga cgacataggc gggaaccgcg ccgagagggt
     1501 cgctcaggta ggagagaacg agtatcggga cgacggcccc cagcactatg tccagcacga
     1561 gctttagcga gcggttcgtg atccgttgcc tctctccggg ttgtggctgt acggcgccca
     1621 gtatgaccgg aaggagccgg gatatccggg gtgggcgcac gtatctcatt ttcttccttg
     1681 cgtagcaaac cgggacaaac cgggacaaag ttcccggcgc tttcgggaca ccgctccgat
     1741 gacggcggga cactcgtagg cgtaaggttc ggttgaagga cgaatgaggc ggcgagtgaa
     1801 ggaagtgtcg tgtgcgtact cacaggtctc gaatcagtgt tccgcagtcc tggctgcgct
     1861 gctacgagga ggatatgctc gtgaaggcgc cgacaatctt gctcgtggag gacagtccgg
     1921 acgacgagtt gctgactatg cgcgctctta ggaagaacgg catgggcgag gcggtggcgg
     1981 tggcccgcga cggcgccgag gctatcgagt atctgcgtaa tgcgcgctcg ctgcccggtc
     2041 tggtgctgct ggacgcgaag ctgccgggta tgagcggtat ggaggttctg aggatcatcc
     2101 gggccgacga gcggatgaag tccctgttcg tggcggtgct gacctcccac gacgagggcg
     2161 ggctgatgac gggcgaccaa tacctgcggg ccgacctgtt catccgcaag ggtgtgaact
     2221 acgatcagtt ttcggatgag atccgcgccc tgaaaccgct cctgaccgag gtgatcgccc
     2281 gccggaatag tccctgcgcc ggtccgattt cttgatgacc gttccgctgt atgaaggttt
     2341 cgtttatagt gttttgaagc aggggtgaca accggaggag ggttccgcgg cgggttggcg
     2401 ggagtaatcc ttcgctgccg ggcaagaggg accatgagag gagagcgcgg catgtccaac
     2461 gagaacaggg atctggtcag gagagcattc gaagcctgcc agcaggcgga caccaccacg
     2521 ttaaaacaat gcttcagcta tgaccgaagt gtacagatac agggcctcgc caggcccaat
     2581 ccttatcccg ccggatcaaa cgtggacctt cactacgata cgccggtcga ggaggcgaac
     2641 agtaaggtac ggaccagcta ccggctgaag acagacgacg gctccgagta ccatgccgag
     2701 gtcgttgaca ccatcgagaa caaaaagatc tcctccagcg tcgttacctg gacggactgg
     2761 gacccttcgg gaaacccgga ggacgacgcg gcctggtacg agggcaagta tgggtggtgg
     2821 ggatggccct tcggcagggg atggtggtag ccggaggaac acggtcgagc gggcagccgt
     2881 gagccggagc ctggggcgct tcgcccgatg aacttgagac accacgcgat caataggcgc
     2941 ggccgcggct ggcggcgcgc gctcgctccg ggacgggact ccgcggtgaa cgcgaacgag
     3001 ccgttaccag ccctgcgcgg gcatcggctc gccgccgcga ggactatatg ggtggttgtg
     3061 gtcctggcgg cgctggcggt cttcgcggcc ggcttgccgg cgtactacga tcagatcgtc
     3121 caggccgcag cggcgaacac tagtaggggc gccgggttgc gggataacct ggggagtctc
     3181 gggctctccg tgggattcta tgcggggtgg ttcggagtcg tgatccccgt cgtgatggcg
     3241 gcggcgttct cgctggtggc ggccgtgatc ttctggcgca ggtccgacga gccgatggcc
     3301 ttgttcgtat cgctgctgct cgcgctcttc ggagcgaccc gctcggacgc caacgaggcg
     3361 ctcgcgcagg tccactccgg cctcggacct ctgagcagcg ccctcgaatc gttgagcgtc
     3421 gtctccgtct ttgccttcct cttcgtcttc ccgaacggac gcttcgtgcc gcgctggacg
     3481 aggtggctgg tggtccccgc cgcggcgtac tctctcctca cctccgtcgt accggactcc
     3541 agtctggacc ccaagcactg gtccggcctg ccgtacacgg ctttcttcgg ggtctggttc
     3601 ctgggtggcg cggtagccca ggtctaccgt taccggcggg tctcgggtcc ggtagagcgc
     3661 cagcagacga agtgggtctt cgccggtttc acggcagcgc tcgcagctta cctcggcatg
     3721 atcctgctct tcttcatcgt cctgacgccg ccggagcccg gcacgctccc ggacttcgtg
     3781 ctgggcacgg tcttctactg ctcgacgctc ttcatcccga tctcgatagg cgtggcgatc
     3841 ctgcgctacc gcctctggga tatagacgtc atcatcaacc gggcgctggt ctacggcgcg
     3901 ctgacggcca tcatcctcgc gctctacgct ctcgtagtag gcggactcgg tttgttcttg
     3961 cagggacgcg gcaacacgct cgcctcgctc ctggcggtcg gactggtcgc cgtgatcttc
     4021 gcgccgcttc gcaaccggct gcagcgcgcg gtgaaccgcc ttatgtacgg tgagcgcgac
     4081 gacccttacg ccgtgctctc gcgcctcggc cgccggctcg acgcagccct cgaaccgggg
     4141 atggtcttgc cgaccatcgt cgagacggtc gcggccgcgc ttaggttgcc ctacgccgcc
     4201 atagagctaa agagccagga cggcttcgag ctggccgccg cccacggaga ccccacggac
     4261 gagcagacct tcctgccgct ctcctatgga ggagagaccg tgggacggct cgtcctcgcg
     4321 ccccgcgcgc cgggcgaggc tttcagcccg gccgacagga gccttctgga ggacctcgcc
     4381 cgcaacgccg aggtcgccgt tcacgccgta cggctcaccg ctgacttgca gcgctcgcgt
     4441 gagcggctgg tcagcgcccg cgaggaggag cggcggcgtc tcaggcgcga cctgcacgac
     4501 ggcctggggc cgacactcgc aagcctcggg ctcggcatag acgtaaccct gaagatactc
     4561 gaagagaacc cggaggaggc cgaggcgata ctgcgccagc tcaagagcca gaccaaggat
     4621 gccgtggtgg atataaggcg gctggtctac gacctccggc cgccagcgct cgacgacctg
     4681 gggctcgttg ccgccatccg ggagcaggcc gcgaaccagg gcatcctagc cagcgaactg
     4741 acctccggcg gggcgaggcg caacagcgga ctcgtattcg agatcgaggc tccagcggag
     4801 cttccgccgt tgtccgcggc ggtcgaggta gcgtgctaca ggatcgtcca ggaggccatc
     4861 accaacatcg tgcgccacgc ccgcgcctca aactgccgcg tgaggatcac ggtagataag
     4921 agcataagga tgcttgagat agaggtgtcg gacgacggga tcgggatgcc ggaagaccgc
     4981 agagcggggg tcggcctgag ctcgatgtcc gagcgggcgg aagagttggg aggcagactg
     5041 ctggtggagc cagcgcccgg tggaggcacg cgcgtgctgg cccggctgcc gctattgacc
     5101 aaggaggacg gatagggtgg ccgaccggcg agacccatca gagaagacgc gggtcctgat
     5161 cgccgacgat catgccctgt tccgctacgg gctaaaggcg atgctgcgct cggagccggg
     5221 tttcgaggtg gcgggggagg cggagaccgg cgagaaggcg gtggatctca cccgcgagct
     5281 aaagcccgat gtcgtgctca tggacataca gatgccgggc atcaacggca tagaggcgac
     5341 gcgcaggatc ctcgagtccg accctgatac aggaatagtc gtagtcacga tgttcgggga
     5401 ggatgattct gtcttcgccg cgatgcgggc cggggcccgc ggctacgtgc ttaagggcgc
     5461 cgacgcggaa gaggtcctga aagtgctgcg tgccgttgcg gacggcgagg cccacttcgg
     5521 gccggacata gcgcgccggc taagagggtt cttctccgcg cctcgtccgc gtcccgagga
     5581 ggccttcccc gagctgaccg cccgcgaacg cgaggtcctg gatctcatag ccagcggcct
     5641 gccgaacgcg gagattgcga cccgcctcta cctgagcgag aagaccgttc gtaacaacgt
     5701 ctcggcgatc ttcctgaagc tgcgggtcgc cgaccgtgcg caggcgatag tccgcgcccg
     5761 ggaggccggc atgggccgcg gcaatgcttg aaagctctac cggcacggag cagtagcgag
     5821 ttgcagcgtt acgctcttgc tggtcttcta tgtagaggtg gcgaactgtt gatcggccag
     5881 ggtccccgac aagaagtcga ctgtggacac gaggtccgag aggccagtac cgctgacgaa
     5941 caggcgccta tcggggtcgt cggcgccgcg gatcgaggcc agccagtagc cgtttgggga
     6001 gagttggatc caggctaccc cgccactcct ggccgccggt gtggtctggc tgcagatcac
     6061 gaggtcgggc gccaggcggg atgcggcgtt ttcgatgcac gccggttccg tggactccac
     6121 ttcgacggat gggtggagcg gtcgcagcgc cgtcgcgaca gcctcccggt gaaagctctg
     6181 ctcggtcgcc aatagcacgc gtagggcgtt catgtaacca acgatacccg gtgcgggagg
     6241 tctccacatg ggccaaacgc gctatttttt tctggcgtgt ttgtgttagc tcatttgagc
     6301 caggacgagc ggggaagtgg atccgggccg tcccttgtgt ctgaccggga ggtgtgaccg
     6361 ggctgtcatc ggccgtccgc cctgactcgc cggttccgca ggaggaggag cccgtacagg
     6421 acgaaaccga gggtgctgat ccagaccagt atcgggtact tggtagagcc cagcgcggcg
     6481 acggtgggga cgtaggccag cgcggcgtgg acgccgacca cgggccagat ggaagcggtg
     6541 cgccaccgga gcgcgccata cgtgaacccg ccgcacgcag ccacggcgct caggcggatc
     6601 gcctcgggaa gcgcgtcgtt ggtggagcgc gctaggtaga gagcgccggt cagcaacgag
     6661 gtgactacga cagcccacac cggaccgcga ggtacgaata ctcgccacag gagcccccgg
     6721 aagaccgctt cttcgccgat ggcggctatg aagaccacga gcagaccggc cacgagcgga
     6781 cccttatccg ggaggaggaa accatccgag agggcgaagg cgcagacagc gagcgggaag
     6841 acgagcaggt agagttttcg ccagcgcggc gcccggaacc cggcctcgcg ccaccagccg
     6901 agcgccgtga ggagcagggc ggcgagcgcg agggcgagca cccagaacag gttgtctgcg
     6961 ttcttgatcg cggtgatcgc ctgttgtagt ggcgagggcg gtttcagggc ctccggagag
     7021 agcccggaga catcgccgat gggagctgat ggtagaaggg tcctggcgac aagagtgagg
     7081 ccgaccagcg ccgcgaggat tgacagggcg aagagcagcg gatggcgttg tgcgaagtct
     7141 ctcaaaaact cagagcctgg ctttcaggcc gctgaggatg aggtcgaggc cgagctcgaa
     7201 ctccgcgtcg aggtccatgt ctcttacctt cggcgagatc tccgcgagcc gggggaattc
     7261 ctccgtagac aggttcgcgc taccaggagg agcggcgccg gattgcaggg agaagtcccg
     7321 gatctccgcc atcgcgtacc cggcggcgta gctggagagc acgcggtaag cgtggagccc
     7381 tgtctcggca tcgaagcccg cgccgtgcag cgtctccagg aactcctcca cgagccacgc
     7441 tccatacatt gtgtccggcg gccgggtgat gaggagtggg aagatgttcg gatgctcgtg
     7501 ggccagacgc cggaaagccc ggtaagcccc gcgcacgcgc tcctcccagt ccttactctt
     7561 cgccggtact tccaactcgc cgagcagcac ttccaccatc ccgtccagca acgcttcctt
     7621 gttcgggacg tggttgtaga gcgacatggc ctccacacca agctccgcac cgagcttcct
     7681 catgctaaga gcctctaaac cctcccgatc cacatatctc accgctgcct ccagaaccct
     7741 ccgccggctt agaggctgcc tcttctcaac gaccaccgac tacctcctta tcgagccgct
     7801 ggtagcttac agtataagtc gtgcaattac attgtaagtc cgcggagaaa ggagcggtgt
     7861 agtgggcatc tttcgctccc ttgcggctgt ggctgttggg tggacagccg ggataagact
     7921 tgcccggaca gtcccgttga atgcgtgatc ttacagcgtt gctcgctggt accgtcgcct
     7981 tgctcccgcg cgcccggaga gaatagttgg atcgggtcga ggtgcaggcc tggcgctcaa
     8041 cctgagagcg gagagctact cgtagaggcc ggcccgctgc ttctgagcct cgatggctgt
     8101 cttgatcctt cgggcgtccg gcagagccat gcgggtcaac ttatagacct gtccggtatt
     8161 ggtctggagg ttcagggtaa cgttgaccac gccgctggtg ccgacggccg cgatgtcgcg
     8221 gaggccgatg cgctccacgt tctggccctg ccagccggtc ctcagctcca cccggtcggg
     8281 gtagacgctc acgatggtat tgttatgtcc agtggcctgc gtgatcggcg cttccaaccc
     8341 tgcctccgtc tgtcctcgaa tacgttattt tgttcaggat acagaatgac cccgaaggag
     8401 tctccccagg ggccctgacg ttcaaaagca ccggacgggg aagtccccgt ccggtgcgct
     8461 gctaaccggc tctctaaatg gtgccttcgg tgacgccctt gggcttcgct tctaccatgt
     8521 cggtagtagt cttgtgtccg ttgccgttct ggtaatcggt cgccacgagg tccgggtatc
     8581 cgaagacgcc gtgctcgctg atgtcgagac cgttcagctc ctgctccggc ttcaccctca
     8641 ggccgatggt cgccttcagg gcggcgaaga ccgcgagtga cgagaggaag acgaatccgc
     8701 cgcaggccac gatcccgagt gcctggacgc cgagctgcgt aaagccgccg ccgtagaaca
     8761 gacccggctt tccgaccgcc gcgagcgccg gggtggtgaa gagacctgtg gacagcgtgc
     8821 cccagatgcc gcccatgccg tgggccgcga tggctcccag agggtcgtcc acgccgatct
     8881 tgtccacgta tacaagcgtc gcgtacatga tgacgcctgc catgaacccg atgatgatag
     8941 aggcccacgg agcgacgaag gcgcacgggg cggtgatggc cacgagagct gcgatagcgc
     9001 cgttgccgcc cattcctacg tcaacgtgac ggcggtacgt atagctcatg agcatagcgc
     9061 cgaggactcc ggcggcagcg gccagggtgg tggtgagggc gatatctgct atctgcgggg
     9121 tggccgccat cgtcgatccg gggttgaagc cgtaccagcc gatccacagg atgagcacgc
     9181 cgaggacggc gagcggcatg ttgtggccgg ggatggttac cgggcgaccc gcatcatcgt
     9241 acttgcccag acgggggccg aggagcaggg tcccggccag ggccgccatc gcgccggaga
     9301 ggtggacgac cgtggaaccg gcgaagtcct gcataccgag ggaggccagc cagccgccgc
     9361 cccagatcca gtgtccgatc agcggataga tgagaccggc gaagacgacc gcgaagacga
     9421 cgtagacggc gaacttggtc cgctccagca tcgtgcccca cacgatggcc agcgacaccg
     9481 cgcagaacgc tacctggaag aggaacttgg ccgagagtgg aacctgcgtc cacgaaaggc
     9541 cggtgatcat cttgtccacc tgcccctcgg ggacgttgag gaagaagccc tgggtgccga
     9601 tgatcgagtt gagcgctcca ccgtcggaga aagtgaacgc gaagccgacg gcccagaaca
     9661 ggagcgccgc tatggcgagg ttcaccagga tcttggcgac cacgcttccc acgttcttca
     9721 tacgcgagaa gccgacctcc agcatcgcga accccgcctg catgaagagc accagcaccg
     9781 ccgcgacgat cacccagatc gtatcagccg ccagggagag atcctcaacg gtgggcttgt
     9841 cttgagcgaa tgcaactgcc ggtagcgcga gtactagaac caacgctacg gttagtagaa
     9901 atcccaaccg acgcaaaaca acttcctcct tgcaatgaga taaacggacg ccgccagcat
     9961 attcttctat gtgaagacag tctttagccc gatgtacaaa gatggcgcct gagaagcccc
    10021 gtgatattgg accttttgta caagcgggcg gagtgacggc tgatgtggcg gctggaaccg
    10081 cggcgaaaga tgcggcggtt ggcggtcgct agcggtcaga gaggctggag ggttgctcgg
    10141 gtacgcggcc gagtagctgc atggccttta taccgagggt gaggcgctcg cggtcctcgc
    10201 tccggtccac gtccaggccg gtaagctccc cgacgcggtc gagccggtag cggatggtgt
    10261 ggcggtgggc gaagaggtcc gaggccgtct tgaccgtcga ggcgtcgttg cccaggtatg
    10321 tgaggagagt ctggaccagt tccgtaccgt agcgggagtc gtagaggacc acgggctcca
    10381 gggtctcgct gtagaaggat tccaactcgg ccgggtcttc ctgcaggacg cggaagagta
    10441 gcttgtaagt gccggtcccc tcgaaggtag agacggagga ggggccgtgg atgcggtgcc
    10501 cgatctccag ggccacctcc gcctcggagt aggcgtccgg cagcgagatc gggtcgtcct
    10561 tgtaccgccc gatgccgatg gagaccgtga ggtccggcag cagccccttg acgtagcgct
    10621 gtatctgggc cgcgagccgg cgggcgttct cggggatctg ggtggcgccg ctatcctccg
    10681 atcctccgag aaacaggatg acgttgtccg agcggggacc gaggaggaaa ttcgtgaaga
    10741 gctggcgggt ctggaggcgc accgcgtcgg cgagccgctt cttcaactcc tggatggcgg
    10801 gctccttcag cttgcgccgc tccaggtagc gggcaaagtg gtctatgtcc acgatcagga
    10861 cgagcgcgcc atcggcgagg tcggccccaa gataccgggc gcgctggagc aacagttcga
    10921 ccgaaccgta atgcccgttg accaggtcgt ccacgaagtc tccccgcacg cccaattccg
    10981 tctccagccg gatgcgctcc ttgcccatct ccgactccag gatgcgccgc gcccagtaca
    11041 gcagcaccac gtcccgcgga ccaagaggct tgtccttcag gtcaatccac atatagcctg
    11101 ccggagcgcc ggtccctatc ggggtccaga aaagctcctc atcctctatg cgccaccgct
    11161 ccaccgggac agagaggtag ccgtccggga ggcgggcgta gcggtcgcgc cgctctcggc
    11221 gcgtctcgtc cacgccggcg ctccgcgaag ctcgcagacc gtgctcttcg atggtctcgt
    11281 gcagaccgct cgtcgcgtcc tgactccagg ccagcagacg gcccaccggg tcctccacca
    11341 cgatctcccg cttcagaagc tccgagatgc ggagcgtcag accttcaatg gaggtatcgc
    11401 cgccggtgct ctccagcagc gtccgtaccg cgccgtgaga gtggagcaag agaccgtctc
    11461 cttcggagaa gagagagagc agacgccgca acgggacctc cggttgcaag acgaagaggc
    11521 cgagccccag agccgcggtc agctccatcg tctcactgtc cggggctgtc tccgaccgcc
    11581 aaaccacggc cggcgcgccc gccccggaga ccgtccgcag gaagtcctcg tccagcgagt
    11641 cgcgggcggt aacggctgct tcgtccggct ccagccagcc cttgataggc tcctcgaccg
    11701 cgacgccgcg caccgtgcgt gagacgtcgc ccggcagcgg gcaatctgcg ccaagttctt
    11761 cgagcaggat cctcgcgaag tccttcagaa gcaagtagtc tccgttcgca acgtagtgta
    11821 accagtcagt atcattgtgg gcgctggcga agcgaatcgc aaatccggtc accgaaacac
    11881 cccgctcggg tgataaaatt ccgggaaaag caatctggtt ctttggcctc cctcaagggg
    11941 gccgtatact tgccaaaaac ttcgcggaat agaggggtga gcagagatcg ccgataacca
    12001 gatcgtcgtg cggggggcgc gcgaacacaa cctgaaagac gtaaccgtct cactgccgcg
    12061 cgacgtgatg gtcgtcgtca ccggtctctc cgggagtgga aagagttccc tggccttcga
    12121 caccatatac gccgaaggac agcgccgtta cgtggagagc ctctcggcct acgcccgaca
    12181 gttcctcggg cagatggaca agccggacgt ggaccacata gacggcctct ccccggcggt
    12241 ctccatagat cagaagacga cgagcaacaa cccgcgctcc acggtcgcca ccgtgaccga
    12301 gatctacgac tacctccgcc tcctctacgc ccgcgccgga cggcctcact gccacgtctg
    12361 cggcttcccg gtcgcctcct ctacgccgca gcagatggta gagaaggtgc tcaccctccc
    12421 ggagaagacg cgcttcatgg tgctcgcgcc ggtcgtgcgg gggcgcaagg gcgagtacgg
    12481 gaagatgttc aaggacctcg ccgaggacgg gtacgcgagg gtgcgcgtgg acggcgagtt
    12541 gcacgagttg cccgtggacc tgaacctcga ccgtaagtac aagcacgaca tcgaggtggt
    12601 ggttgaccgg ctcgtgatcc gggagggcat ggagcgccgc ctgacagact ccatcgagac
    12661 cgcgctgaag ctggccgagg ggctcgtaca gatcgagacc gtcgagacgg acggcccagg
    12721 ggagtcgatg ctcttctcgg agagcttcac ttgcactaac tgcggcgcct cgatagcgga
    12781 gata
//
LOCUS       DASSMB010000053         3796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_13275, whole genome shotgun sequence.
ACCESSION   DASSMB010000053 DASSMB010000000
VERSION     DASSMB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13275"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VFJ72_03650"
     CDS             complement(<1..698)
                     /locus_tag="VFJ72_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET7478597.1"
                     /translation="MARGTHEALPDERNADVLIYINGEFYPRAEAKVSVFDSGFLVGD
                     GVWEGIRLHDGEFAFVELHLDRLFEGAKAIDLDIGMTREEVADALRETVERNGMTTDI
                     HIRLMVTRGDKKTPSQDPRLVVGGPNVVIIAEHKLADPEVKERGVRLFTSTVRRPPPD
                     TLDQRLNCHSKLHEVVALIQALKAGAEEALMLDPTGAVATCNATNFFIVRRGEVWTST
                     GHYCLNGITRRMVI"
     gene            complement(714..2243)
                     /locus_tag="VFJ72_03655"
     CDS             complement(714..2243)
                     /locus_tag="VFJ72_03655"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HET7478598.1"
                     /translation="MTTSSRDPLQLLNDRLATISDISAAAAVLNWDQQTYMPEGGVQG
                     RAEQLSTLSRIAHEMLASDETGELLDSAGEPEPGSEDAATIRLTRREYERATKLPSRL
                     VAELSRARALAEPAWARARAESDWSAFAPHLEKIIPLTLETAEALGYEDHPYDALLDI
                     YEPGARKAPLAKMFEELKTGLVPMIHDIGSAAAEDRSAPLFGEFDEAKQEEFGKAVIS
                     DFGYDWSRGRQDRAVHPFCINFGGPGDVRITTRFDNGWLSPALFATFHEAGHAMYEQG
                     VDPAYTRTALSGGTSMGIHESQSRLWENLVGRSRSFWSHYYPKLRETFPASLGETSLE
                     AFYRAINDSRPSEIRVEADELTYNLHILLRFELEVALLEESISVEDIPAAWNAKMEEY
                     LGLTPKTDAMGALQDVHWSAGLFGYFPTYTIGNVLSVQFFDTAVKERPEIPDGIERGE
                     FDVLREWLRENIHRHGSRYDPEDLVERVTGRPLETGPYLRYLREKFGELYEPGYSRRP
                     G"
     gene            2369..3742
                     /locus_tag="VFJ72_03660"
     CDS             2369..3742
                     /locus_tag="VFJ72_03660"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7478599.1"
                     /translation="MNGFLKGVLAVGGGAVAAETLRRVIKDGARPRYEAWERAPYRDF
                     PNRILILGGGFAGYTAAKSLCKMTRHRDDVGVMVISRENYFTFWPMLAGCISSDIDTK
                     NVAQPLRRALIQHGASFRRAEVESIDFERQIVTAEGREFPYDHLVLALGAEPAYFGIP
                     GVEQHAISIRGIGAAEEIRNRVIERYEEATLSTGEVSDARLTFVIIGGGATGVEVASE
                     LHALVHDILAPDYPNINPHRVKIVLVDSNEQVLKELDPALRRTARKKLADLQIQVLNK
                     VKAKEITSDKVVLSDGKEIATENAIWTAGARASEKLDDIDLPSDERKGLKTDRYMRVE
                     GYENVWGIGDCAMTVEEDGTPVPPNAQAAVQEGRVVARNILASIDGGALHTFDYKPVG
                     QLVEMGSEFAVNQVMGVKFSGLVAALFWRATYLYKLESPQSRARIAADWFLDLFFSPA
                     VTQIRER"
BASE COUNT          623 a         1214 c         1299 g          660 t
ORIGIN      
        1 tcgatgacca tgcggcgggt gatgccgttg aggcagtagt ggccggtgga ggtccagacc
       61 tcgccgcgcc gcacgataaa gaagttggtc gcgttgcagg tggctacggc gccggtaggg
      121 tcgagcatca gggcctcttc ggcgccggcc ttcagggcct gaatgagcgc gacgacctcg
      181 tgcagcttgc tgtggcagtt gagccgctgg tccagcgtgt ccggcggtgg gcgtcgcacc
      241 gtcgaggtga agagccgcac gccccgctcc ttcacctccg ggtcagcgag tttgtgctcg
      301 gcgatgatca cgacgttcgg gccgccgact acgagccgcg ggtcctgcga cggcgtcttc
      361 ttgtcgcccc gcgtgaccat cagccggatg tggatatccg tcgtcatccc gttccgctcg
      421 acggtctcgc gcagggcgtc cgcgacctcc tcgcgcgtca tgccgatgtc caggtctatc
      481 gccttcgctc cctcgaagag ccggtccagg tgcagctcca cgaaggcgaa ctcgccgtcg
      541 tgcagccgga tgccctccca cactccatcc ccgaccagga agccggagtc gaagacggag
      601 accttcgcct ccgcccgcgg atagaactct ccgttgatgt agatgaggac gtccgcgttg
      661 cgctcgtccg gtagtgcctc gtgcgtgcct cgcgccatct gattccctcc gcctcagccg
      721 ggtcgccggg agtagcccgg ctcgtatagc tccccgaact tctcccgcag gtagcgcagg
      781 tacgggccgg tctccagcgg tcggcccgtt acccgctcga ccagatcctc cgggtcgtag
      841 cggctgccgt ggcggtggat gttctcgcgc agccactcgc gcaggacgtc gaactctccc
      901 cgctcgatac cgtccgggat ctccggtcgc tccttgaccg ccgtgtcgaa gaactgcacc
      961 gagaggacgt ttccaatggt gtacgtcggg aaatatccga agagtcccgc cgaccagtgt
     1021 acgtcctgga gtgcgcccat cgcgtcggtc ttcggcgtca ggcccaggta ctcctccatc
     1081 ttcgcgttcc aggccgccgg gatgtcctcc acggagatgc tctcttccag cagggcgacc
     1141 tccagctcga agcgcagcag gatgtgcagg ttgtaggtaa gctcgtcggc ctcgacccgg
     1201 atctccgacg gccgcgagtc gtttatggcc cggtagaagg cttccaggct cgtttcacca
     1261 agagatgccg ggaaggtctc ccggagcttc gggtagtagt gggaccagaa gctgcgcgag
     1321 cgccctacca ggttctccca gagccgggac tgggattcgt ggatgcccat cgaggtcccg
     1381 ccggagaggg cggtgcgggt gtaggcgggg tccacgccct gctcgtacat cgcgtggccg
     1441 gcctcgtgga aggttgcgaa gagagcggga gagagccagc cgttgtcgaa gcgggtcgtg
     1501 atcctcacgt cgcccggccc gccgaagttg atgcagaacg ggtgtacggc ccggtcctgg
     1561 cggccccggc tccagtcgta gccgaaatcc gaaatcacgg ccttcccgaa ctcctcctgc
     1621 ttcgcctcgt cgaactcccc gaagagcggc gccgagcggt cctccgccgc agcggacccg
     1681 atgtcgtgga tcatgggcac aagcccagtc tttaactcct cgaacatctt cgccagcggc
     1741 gccttgcgcg ctccgggttc gtagatgtcg agcagggcgt cgtagggatg gtcctcgtag
     1801 cccagagcct cggcggtctc cagggtgaga gggatgatct tctccaggtg cggcgcgaag
     1861 gcggaccagt ctgactccgc gcgcgcccgc gcccacgccg gttctgccag cgcccgagcc
     1921 cgcgagagct ccgcgaccag ccgagacggg agcttcgtgg cccgctcgta ctcgcggcgg
     1981 gtcagccgga tggtcgccgc gtcctccgag ccgggctccg gttcgccggc ggagtcgagc
     2041 agttcgccgg tctcgtcgga ggcgagcatc tcgtgggcga tgcggctcag ggtggagagc
     2101 tgttcggcgc gtccctgcac gccgccttcg ggcatgtaag tctgctggtc ccagttcaat
     2161 acggctgctg cggcgctaat gtcgctgatg gtggcgagtc ggtcgtttag aagctgtaac
     2221 ggatctctgg atgaagtagt cataatctca tactagcatc ccgcgccacg gggcttgctg
     2281 gaggaaatgt tcttcgtggc tgtagtggcg ttgggcgtcg cagggtagga caataggcaa
     2341 gtcgggtcgg ctccgaaagg cgggtttgat gaacgggttt ctgaagggag tactcgcggt
     2401 cggcggaggc gcggtagccg cggagacgct gcgccgggtg atcaaggacg gagcgcggcc
     2461 ccgctacgag gcgtgggagc gggcgcccta cagagatttc ccgaaccgga tcctgatcct
     2521 gggcggcggt ttcgccggtt atacggcggc caagtcgctg tgcaagatga cccggcaccg
     2581 cgacgacgtt ggggtcatgg tgatctcgcg ggagaattac tttaccttct ggccgatgct
     2641 cgcgggctgc atcagcagcg acatagatac gaagaacgtg gcccagccgc tccggcgcgc
     2701 cctcatccag cacggcgcca gcttccggcg cgccgaggtc gagagcattg acttcgagag
     2761 gcagatcgtg accgccgagg gccgggagtt cccctacgac cacctggtgc tcgcgctggg
     2821 cgcggaacct gcgtacttcg ggataccggg ggtcgagcag cacgccatca gcatcagggg
     2881 catcggggcg gcggaggaga tccggaaccg cgtcatcgag cgctacgagg aggcgacgct
     2941 ctcgacgggc gaggtctccg acgcgcgcct gacgttcgtc ataatcggcg gcggggcgac
     3001 cggggtggag gtcgcgtccg aacttcacgc cctggtccac gacatcctgg ctcccgacta
     3061 cccgaacatc aacccgcacc gcgtcaagat cgtgctggtg gacagcaacg agcaggtcct
     3121 caaggagctg gaccccgctc tgcggcgcac ggcccgcaag aagctggccg acctccagat
     3181 ccaggtcctc aacaaggtca aggcgaagga gattacgtcg gacaaggtcg tcctctcgga
     3241 cggaaaggag atcgcgaccg agaacgccat ctggacggcc ggcgcgcgcg cgagcgagaa
     3301 gctggacgat atcgacctcc cctccgacga acgcaagggt ctgaagaccg acaggtacat
     3361 gcgcgtggag ggctacgaga acgtgtgggg catcggggac tgcgcgatga ccgtggagga
     3421 ggacggcacg ccggtcccgc cgaacgcgca ggccgccgtg caggagggca gggtggtggc
     3481 gaggaacata ctcgcttcta ttgacggggg agcgcttcat accttcgact acaagcccgt
     3541 cggacagctc gtggagatgg ggagcgagtt cgccgtcaac caggtcatgg gcgtgaagtt
     3601 cagcgggctc gtcgcggcgc tgttctggcg ggcgacctac ctgtacaagc tggagagccc
     3661 gcagagccgg gccaggatcg ccgccgactg gttcctcgac ctcttcttca gcccggccgt
     3721 cacccagatc cgggagcgct agtagccttc acgggccgtt cgtgatccag acggtctcgt
     3781 aagggctcag tctcac
//
LOCUS       DASSMB010000054        23567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_140258, whole genome shotgun sequence.
ACCESSION   DASSMB010000054 DASSMB010000000
VERSION     DASSMB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 23567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23567
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140258"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VFJ72_03665"
     CDS             <1..1033
                     /locus_tag="VFJ72_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7478600.1"
                     /translation="VSGSNPLSTQDDVCAALAERGVRVYATHDPSDEDFERYLHQTIE
                     TGPELIIDDGAELVGRLIESHPELVDNVIGASEETTTGVTKLKAMTAEGVLPFPVLAA
                     NDARMKHLFDNRYGTGHSSIVALLSNTNLFLSGKSVVVMGFGWVSRGLAKYADGMGAR
                     VIVCESDPVKLLEAYSEGYDVMNSMKAAEVGDVFLTGTGNLRVLGKQHFELMKDGALL
                     ANAGHYDHEFDLAGLQEMAVESREVRRNVDEYELSDGRRLHVLARGRLVNIAAADGHP
                     VEIMDLTFAVQALAAHHLVSHAADIPAGIQPIPAEIDDLVARAKLASLGVEPETLTEE
                     QISYQQSWH"
     gene            1099..2310
                     /locus_tag="VFJ72_03670"
     CDS             1099..2310
                     /locus_tag="VFJ72_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478601.1"
                     /translation="MTKKTPAATGTGGGLRSALLVVGVVLLAANLRPAIIAVASLIGE
                     IRPDAGLSNGTAGLLTTLPLLAFGLLSPVAPRLARRFGMERVLFAGLVVLAAGILLRS
                     QGTVAALFLGTATLGAAIAVGNVLLPAVIKRSFPDRVGLMTSVYVTALAVSAALAAGV
                     SLPISDQLGAGWKTALAVWAIPAFVAAAVWLTQLGRGRQVDGPDGTSGAPGKVSGLWR
                     SALAWQVTLFMGLQSLAYYAALAWLPEILRDGGMGAAGAGWMLALAQTVGIAAMFLAP
                     VLAGRTPSQRGVVVASVALSGAGVLGLLVGGIGLAPVWVALLGLGQGACFSLALTFFA
                     LRSADPAHAAALSGMAQSVGYLLAAAGPFLFGILRDATHAWTVPLMLLLTIVVCLLIT
                     GLGAARDAQVG"
     gene            complement(2307..2732)
                     /locus_tag="VFJ72_03675"
     CDS             complement(2307..2732)
                     /locus_tag="VFJ72_03675"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7478602.1"
                     /translation="MNPALVLAVLAGVAVGFQVVANTTGMKSLGIGGLVAISGFTTGV
                     IGFLVASFMTRPELTGKAVTCAIASGILGAFIVSSITLAAGQTGVAQTLSLVIAAQLI
                     MGLVIDRLGVYGPAAAEIGLVKVLGIVFILIGGVLVVRS"
     gene            2780..3085
                     /locus_tag="VFJ72_03680"
     CDS             2780..3085
                     /locus_tag="VFJ72_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478603.1"
                     /translation="MRTITIRYTEFRECPNCEGRGDDGSAWRPFECRRCRGRGRLITG
                     VEVEDSVEGLLPRSRYDFTALGEVYLLNFDEAELEEIEVIRPENGSGPRAPSAGDPE"
     gene            complement(3027..3476)
                     /locus_tag="VFJ72_03685"
     CDS             complement(3027..3476)
                     /locus_tag="VFJ72_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478604.1"
                     /translation="MKRDLRSLAILTVGCTFAAALFGFGSEMFSFRSAYEGSSYGTLI
                     LLTRLIVYLVLAATLVFRGGWWGVLAAVVMTVGATVIEWALFPAAFQWAAISDPAGYR
                     KEYGDAVSRPDYGRWATFDIIGISIAAAFTQGLRLMAHVDPNRSPDE"
     gene            complement(3518..4756)
                     /locus_tag="VFJ72_03690"
     CDS             complement(3518..4756)
                     /locus_tag="VFJ72_03690"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HET7478605.1"
                     /translation="MLRSEHVMARVSRGRVVPHRLSTSDRRVLEIAAELCDLYAGHLG
                     QPRAALQAALAEREEELGPRLDARRGFRIVRALAKLLEERASWEPPTEADPYTVRTRL
                     FELAAALPEPPAAEPGLLAATTRDDLLSQVSIETTLVDPSAVMYADRQAAQLLSVFDR
                     PEPEALVLRYNVAQAQGVLYSARDLIVDLGPGADARLVFHYVKLMGLVYRLETTSRGY
                     RLHLDGPLSIFGGTRKYGLRLAKFLPGLLLTTPWSLSANVEWKGRDAVLELDSESGLR
                     SHYLGPREAREPDEVREAFVKAWERAKDTGGWRLDAGAEILPLPELKAALVPDFALTS
                     DTGERAYLEILGFWSERSLIERVALVREARRRGHRVLLAASENLSATKETLSEAVHGE
                     VVPFKNRLAPKAVLEALTDL"
     gene            complement(4756..6132)
                     /locus_tag="VFJ72_03695"
     CDS             complement(4756..6132)
                     /locus_tag="VFJ72_03695"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HET7478606.1"
                     /translation="MIELFYESGTLIASGLPDGDHPPEPFIWDGRTRQWRAPASAYRE
                     VSLSLRGSSTPHRDRAAAFEKLSLESRVAMEPRPYQRDALAAWRRADLRGVVVLPTGA
                     GKTAVAVRAIERVGRSALVVVPTLALLKQWYSILQDAFGDSVTVGLLGGGYHEVTPLT
                     VTTYDSAYIHAERYGDRFALAVYDEVHHLPAPKNSVISKMLLAPYSLGLTATPERPDG
                     GHDLLPELVGPVVYHLSPEDLAGTYLAPYDLIRIPVELTAHERHEYAEAEAIYRDFLK
                     KHHLSVRSPEDWQRFILVAATSHSGGREALLAARRRREIQAGAVRKGATLENLLRRHW
                     DDRCIVFTKSVEEVYALSRRFLIPGITFETPARERKEILDRFREGRYRTIIASDVLNE
                     GVDVPDANVAIILAGSASHREYVQRLGRILRPKEGKRATLYELVTTATGEEFTARRRR
                     ESTRGIPV"
     gene            6205..7389
                     /locus_tag="VFJ72_03700"
     CDS             6205..7389
                     /locus_tag="VFJ72_03700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HET7478607.1"
                     /translation="MKHPGRGGWAALVCGMVVVIGVVAALLAGCGGMSGDASGTDAKS
                     SSAQQEKKTEDKPAAGEMSVRDMVGQMFVVSMGGTEPDYYIEKMVRERNIGGVLLFGY
                     NMKSEEQVKSLTASLQKLSMKTKPAIPLFIGVDQEGGRIASAPWVAPQPSAAEIGQRG
                     DPVEAHNVAEKMGTELLRAGVNTDFAPVVDTGFGAAIGDRSYGEDPLLVSKMGAAAVK
                     GFEAAGVVAAAKHFPNHGPATSDSHVSLPVVDHDMATIRSYDLPPFQAAIKVGVPMVM
                     AGHLVYPALDPDNPASLSPKAIGMLRGDLGFDGVVITDDLAMKGANGGGPPARAAVEA
                     VKAGADMLIISSPPQEQADAYDAVVEAVQSGEIPEKQIRASVARVLKVKEKYSLYGEK
                     SR"
     gene            7514..8761
                     /gene="ugpC"
                     /locus_tag="VFJ72_03705"
     CDS             7514..8761
                     /gene="ugpC"
                     /locus_tag="VFJ72_03705"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET7478608.1"
                     /translation="MAEVTLEDVTKIYGEDVVAVDKFNLDITDGEFIVFVGPSGCGKS
                     TALRMIAGLEDISRGKIFIGDNVVNDLPPRERDIAMVFQNYALYPHMNVRENMGFALK
                     LRKMDKNEINRRVDEAARILSIERFLDRKPKALSGGQRQRVALGRAIVREPKAFLMDE
                     PLSNLDAKLRVEMRTSISKLHNRIGVTTIYVTHDQTEALTMADRIVVLKDGKVQQIDS
                     PQVMYENPNNIFVAGFIGSPSMNFIRAHIEQDNGGYAVKFGPTSLPISREAAGESKER
                     RGRDIGEYVGKEVVLGIRPEHIEDASVEEAAALINADHTTSMEVEPQVIESMGSEKYL
                     YFEIDRSQTIHLDSVSDMTDDSAADEESGGTSGTSGSVAGGEMMTARISAASKARLGE
                     KMELVIDASKMRLFDPETEEAIL"
     gene            8847..10196
                     /locus_tag="VFJ72_03710"
     CDS             8847..10196
                     /locus_tag="VFJ72_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7478609.1"
                     /translation="MTERQERRPETLSGRRINRRDFLKIGGAGLAGAALLGVAGCGGG
                     GGGGSSGGSGDIVFAMGPDTSGSLQKLIDKFNKENKGKYSVKYRKMPTDTGQFFDKIR
                     TQFQSGGGDIDVIGGDVIWPAQFAANGWISDVSDKLSDSERSKFLDGPIASMTYQGKL
                     YGIPWYTDAGMLYYRKDLLQKSGFNEAPKTWDELKSMAKKVVQDSGTKAGFVWQGSQY
                     EGGVVDGLEYINSYGGKVLDPSDASKVVIDSPDSVKGLAMAASMIPDGVSPQAVTNYT
                     EQESQTAFLNGEAVFCRNWPYMYALVGNPDESKIKTDQVSVAPLPAGSAGSVSGLGGW
                     NFYINAASQKQDAAIAFAKFMADPEQQKYRALNGSFLPTLKSLYDDKEILDAVPVIAL
                     AGEALANTVPRPVSPAYSDMSLKMAEQFNKAFKGDVSPEDALKTLQTDLQQIADQAS"
     gene            10258..11169
                     /locus_tag="VFJ72_03715"
     CDS             10258..11169
                     /locus_tag="VFJ72_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7478610.1"
                     /translation="MTTVSREKKRGWLKEEFGNPERRTAYYMVLPALLIILLIAFYPV
                     INSMWLSVHQSTITQTGPFNGFENYAGIFTNTDFLGGLFNTVVFTVISVAFEFVIGLG
                     IALAINRAFRGRGLVRAAVLVPWAFPTVISAAMWRLMFQDQVGVINTVANGVGIISEP
                     ILSDRTLLLIASILVDVWKTTPFMALLLLAGLQTIPADVYEAARVDGANVMQRFFQIT
                     MPLLKPTILVAVLFRTLDSYRVYDLFWVMSNRQLESLSTFVYKGVRISQLQFAQGNAA
                     AVFIFITAFLLALFFIYGLGMQTSTEE"
     gene            11172..12029
                     /locus_tag="VFJ72_03720"
     CDS             11172..12029
                     /locus_tag="VFJ72_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7478611.1"
                     /translation="MASATATGGSRSGSANMILFYVFLLLFVLVSLFPLLWVFKMSIV
                     TKSDLFQSPVTTLVAPPISDNYGTIFSNPDFRRALLNSSVIAGVTTIICLFFGAICAY
                     AIARLRFRFKSAVMTTILALSFFPAVAIIAPLFVLFSNTGLINTYWSVIVTDTVFALP
                     LTIWILVAFFKELPKDLEEAAKVDGATTIQAFRKVIVPLAAPGVFTTAILTFIFAWNE
                     FLFANTFLFDASTQPVTVAIPNFATVYTVDYGAQAAAAVVVTIPLVILVLLFQRRIVS
                     GLTAGAVKG"
     gene            12135..13061
                     /locus_tag="VFJ72_03725"
     CDS             12135..13061
                     /locus_tag="VFJ72_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7478612.1"
                     /translation="MSLRSTFLATDCVVRNDSRGAPYLSLKLVDRTGSIDARMWGLPF
                     ELRSGFSGPEYVQVEGNAHEYRGTLQVKIDRLKVLGREEISEEDYLPATERDRQALAA
                     ELEMCGRELEDEHLRTLFEKIVADEDFWEAFCAAPAAKGMHHARIGGLLEHSVQCMRI
                     ARGISDLYPVKRDLLIFGAIFHDAGKTKELSWGGGGFAYTTEGRLEGHVVLGERLMSS
                     YIAEIPEFPEELKLQISHILLAHQGEIEYGSPTQPKTLEALLVHLIDNLDARAEMFLQ
                     STENVSAGGWSHHENPLKRALYVPQEKEIEGE"
     gene            complement(13076..13297)
                     /locus_tag="VFJ72_03730"
     CDS             complement(13076..13297)
                     /locus_tag="VFJ72_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7478613.1"
                     /translation="MFGVGPQEMVMIGLLALLIFGPSKLPQMARDLGKFVAEARRSID
                     EFKDELTAEHDDEDEDDDSKDGKDGRRDL"
     gene            13434..14447
                     /locus_tag="VFJ72_03735"
     CDS             13434..14447
                     /locus_tag="VFJ72_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET7478614.1"
                     /translation="MISWAGVRTIAVALASRSERVPAGDFDYPGAVERTLDPLSRFTG
                     ISIPPSGRQLRIADRAEWIDFNIEGFGALMEPVLKRASAGADSVTWFLGGVTLTAQVG
                     LLLGFLSARVLGQYDTGPLLVRERSGEPGKVFFLDGNIVSAAGRIGVPVDGLRLWIVL
                     HEMTHALQFEGYPWLRSYLGDLLESLISPLAERLGARETLRRISRNLSTGGRSVELMM
                     SVPQREAFDRMQAAMSVIEGYSDYVMHHVGRGLVPNYENLSRRMARSRAHRPPLETAI
                     FRITGMDMKLEQYRLGEAFADAVVRRQGMQGLNRVWERPENLPTLQEVRDPGLWMSRM
                     ETA"
     gene            14449..14784
                     /locus_tag="VFJ72_03740"
     CDS             14449..14784
                     /locus_tag="VFJ72_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478615.1"
                     /translation="MSEMSGEERRPAQTVSYEFATENAARDAARELDRRFDQKELLAL
                     RIYRVRWNGNYLVEASFDPGAQDTRLRDARALLGESGRPVHPDDLEDYKRATREGGGL
                     SGWFRSFFG"
     gene            14833..15093
                     /locus_tag="VFJ72_03745"
     CDS             14833..15093
                     /locus_tag="VFJ72_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478616.1"
                     /translation="MAESGILSEADRVEFIEGEVVEVFKTLLDYDVEVKVPLYARARI
                     PEVWIVGLFAGEIYQSVERLRREELTSSAVPGLTVQTGDIFG"
     gene            complement(15097..15390)
                     /locus_tag="VFJ72_03750"
     CDS             complement(15097..15390)
                     /locus_tag="VFJ72_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478617.1"
                     /translation="MREGSAKFVFGVLVGAAGGFAVGSFAATPTARSAGQSALGGLGG
                     SARYVGRATVRTVHHLGTAIESGYTRVRGREKYLEHEIEELREQITRLEQRMD"
     gene            15496..16863
                     /gene="selA"
                     /locus_tag="VFJ72_03755"
     CDS             15496..16863
                     /gene="selA"
                     /locus_tag="VFJ72_03755"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HET7478618.1"
                     /translation="MLETGGHERLRNLPAVDAVLRSPHAGALAHRHGRAALTAAVRES
                     LASMRRSILAGELPEVSEERVLSSAARMLSTRGLRPVVNATGVILHTNLGRSVLSERA
                     VEAVTEAARSYSNLEYDLTSGSRGSRYDHAVPLLRALTGAEDALVVNNCAGATLLALA
                     SLVGETGNGEAPEVIVSRGQLIEIGGGFRIPEVLALSGARLREVGTTNRTRLADYEGA
                     IDENTRAVLWVHPSNFATVGFTESVGVAELARLEVPVIADVGSGAMFAMGDEPRVQES
                     LRDGAALAIFSGDKLLGGPQAGIAVGRSGLISAMRRHPLARALRADKLCLAALYATLT
                     AYLEGTATEEVPTARMLHASTSEVEVRATALGAALLRAAPEMEIDVAPSVARSGGGTM
                     PVYEIPSFAVRIAGDADSLAARLRAADPPVIGRVHEGRVWLDVRTLLPGDEEAVERAV
                     RGLRG"
     gene            16856..18703
                     /gene="selB"
                     /locus_tag="VFJ72_03760"
     CDS             16856..18703
                     /gene="selB"
                     /locus_tag="VFJ72_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HET7478619.1"
                     /translation="MADRPIALTIGTAGHVDHGKTTLVRYMTGTDTDRLEEEHRRGIS
                     IVPGYAELDLPGGRRASLVDVPGHERFVKNMVAGSTGVDAFLLVVAADDGVMPQTREH
                     LDILRVLGVERGVVALTKVDAVDEEMADLAELDVDELLESAGGEAPVVRVSGVTGEGV
                     DELLQALDELAATPSGRGSSDLARLPVDRAFVLKGIGVVVTGTLWSGEIHIGDTLYAS
                     GHRPRVRGIQNHGHPAKVAHPGARTALDLAGVEPSQIQAGDVLLSRSLSPTRDLDVRI
                     RLLETARPLAHGVRVRLHHGTRDTNARVRLVDRGEIGPGDSALARLKLEEPLVALSGD
                     RFVLRSMAPQITIGGGTVLDAAPTGRRPDPVWLEALEGGETERLVSMALSRPPREGMT
                     AGDLSLVLGIPPAEVLAAAHSNVEVEELGELLVPAEEVAAARRRLLQALRLRAEERPE
                     NPELTVAEARTATGLGAGAADALLAEMAAETIRITDSGVSLPDANEAPPELENEAREL
                     LTELKGAGVEPPALEPTPALRLLVKRGEVVDLGGKIYGSREAAEAVIEEIKAHCREDG
                     DISLAGLRDRLKTSRKYAQAWLEYADTAGVTRRIGDVRVLTRRYRQAMD"
     gene            18727..18815
                     /locus_tag="VFJ72_03765"
     tRNA            18727..18815
                     /locus_tag="VFJ72_03765"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18759..18761,aa:Sec,seq:tca)
     gene            18845..19552
                     /locus_tag="VFJ72_03770"
     CDS             18845..19552
                     /locus_tag="VFJ72_03770"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7478620.1"
                     /translation="MPDTFEVITYNAWCERGGRDPLLDDALEDGAIVCLQEVKPRRAL
                     ELKRRFGKRAFVSPGRHGLQYIALVLPRDARFVRRRTVQLNGIVGLVPRRWSLRRGAA
                     MRRAGRPGWRDCFEPRVAQEADVEWRDRRVRVVNTHVPYAPLLRDPCLGMLAALTGEG
                     DAILAGDLNAPAESLHLNDLVLATGLRTTGMGKATHGRHKIDHVLVRGPFREAGYRTR
                     EGKSDHRLLKVVLEVNE"
     gene            19552..20010
                     /gene="moaC"
                     /locus_tag="VFJ72_03775"
     CDS             19552..20010
                     /gene="moaC"
                     /locus_tag="VFJ72_03775"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET7478621.1"
                     /translation="MVDFSHIDREGSARMVDVGEKAVIRRTAIAGGHVLMAPETIELL
                     RDRALPKGDALNTARIAGVMAAKRTPDLIPLCHGLNLTSIDVDFEVAGDRIEISATAR
                     ASDRTGVEMEALTAVSVAALTIYDMCKAVDKNMTIGEVRLIEKTKESIPE"
     gene            20179..20703
                     /locus_tag="VFJ72_03780"
     CDS             20179..20703
                     /locus_tag="VFJ72_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HET7478622.1"
                     /translation="MDLSGRRCVVVGGGAVANRKARKLLQARAEVVVISPEVQPELAS
                     MAREVRRRPYQMGDLEGAYLAFTATDDREVNAAVAREARERSIPINVADNPAEGDFAL
                     PSTLRRGRLQVSVSTGGASPTLARRIKDELEKLFGPEWSGLVEELERGRRRGEKAEER
                     LEGEVSRCLSQLRG"
     gene            20679..21974
                     /gene="hemA"
                     /locus_tag="VFJ72_03785"
     CDS             20679..21974
                     /gene="hemA"
                     /locus_tag="VFJ72_03785"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET7478623.1"
                     /translation="MLIAVAGMSHRSAPVEVRERVAFAPCTARDFLRRLKDDGVVSEA
                     VLISTCNRTELYAVVEDEVAREKMLDALAEERDVDRASLDDDTYWLTDAEAVRHLYRV
                     ASSLDSMVVGEAQILGQVRDAYRIATEEQCAGPILNRLFHTSLRVGKNVRTQTGIGDS
                     SISVPHVAVKLAAEVFGTLAGRRALVLGAGDMSELVIKHLKSRGVADIRIANRTPERA
                     RLLAERIGGRAVAFDALAEQLAEVDVVVSSTGSGDWVVGADLVSDALDGREEPLFFVD
                     IAVPRDVDPAVQALERAYVYDIDDLQSVVDYNAEGRGAAAESGEAMISPAVFEYMSWL
                     STLHVVPLIQELRSGAERIRRHELSRALKKMDLSPEDAASVERLSHSLVNKLLHGPIA
                     ELKALAESGAPLDSAEVRQRLLALESISVELHRSGPTAP"
     gene            21971..22894
                     /gene="hemC"
                     /locus_tag="VFJ72_03790"
     CDS             21971..22894
                     /gene="hemC"
                     /locus_tag="VFJ72_03790"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET7478624.1"
                     /translation="MTPGQSVVLGTRGSKLALTQAESCARRLRDAGLDVEVRAIKTTS
                     EQRPETPFSVIDQRDVFTRQLDEALLAAEIDLAVHSLKDVPTTLREGISLAAISERHD
                     PSDALISEERYDLDTLPEGAVVATSSLRRRAQVLNRRPDVSIVEIRGNVDTRVRKLRE
                     GAADALILARAGLDRIGLDAPHTVIPTEVMLPAVGQGALAVAALPDHPLYGHIREHLN
                     DEITERATTAERALLRTLEGGCRVPVGALGAVRGDGVYLRGLVASIDGALVYRGEAEG
                     EDPDEVGERLARDLLEQGAAVVLGEVREVRS"
     gene            22891..>23567
                     /gene="cobA"
                     /locus_tag="VFJ72_03795"
     CDS             22891..>23567
                     /gene="cobA"
                     /locus_tag="VFJ72_03795"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET7478625.1"
                     /translation="MTVYLVGSGPGDPGLFTVKGVRCMREADVVVYDRLAPESLLSHA
                     RPDAERIYVGKKPGNPTMTQDEINALLVELGRAGKNVVRLKGGDPYIFGRGGEEALAL
                     IDAGIPFEVIPGVTSGVAAPAYAGIPVTHRGVSTSVAFITGHEDPTKGRSDVDWRKIA
                     NGADTLVLYMGVGRLAEISAALISAGKSPDTPVACVRWGTRPDQETVTGTLKDIADLV
                     AEANLKPP"
BASE COUNT         4098 a         7371 c         8167 g         3931 t
ORIGIN      
        1 cgtatccggg agtaacccgc tctcgaccca ggacgatgtc tgtgctgcgt tggcggagcg
       61 cggcgtccgg gtctacgcga cccacgaccc ttccgatgaa gacttcgagc gctacctgca
      121 ccagaccata gagaccggcc cggagctgat catcgacgac ggcgcggagc tggtggggcg
      181 gctgatcgaa tcgcatccag agctcgtaga caacgtcatc ggcgcgtccg aggagacgac
      241 caccggcgtc accaagctga aggcgatgac cgccgagggt gtgctgccct tcccggtgct
      301 cgccgccaac gacgcccgca tgaagcacct cttcgacaac cgctacggca ccggccactc
      361 ctccatcgtc gccctgctct ccaacaccaa cctcttccta tccggcaagt cggtcgtggt
      421 gatgggcttc ggatgggtta gccgcggcct cgccaaatac gccgacggaa tgggagcgcg
      481 cgtgatcgtc tgtgagtccg acccggtgaa gctcctggaa gcctactccg agggctatga
      541 cgtgatgaac tcgatgaagg ccgccgaggt cggggacgtg ttcctcacgg gcaccggcaa
      601 cctccgggtg ctcgggaagc agcacttcga gctgatgaag gatggcgcgc tgctcgctaa
      661 cgccggccac tacgaccacg agttcgacct cgcggggttg caggagatgg cggttgagtc
      721 gcgggaggtg cggcgcaacg tcgacgagta cgagctttcg gacggtcgca ggctgcacgt
      781 gctggcgcgg ggacggctcg tcaacatcgc ggcggcggat gggcacccgg tcgagatcat
      841 ggacctcacc ttcgccgtgc aggcgctcgc ggctcaccac ctcgtgagcc acgcggcgga
      901 catcccggcg ggcatccagc cgataccggc cgagatagac gacctcgtag cccgcgcaaa
      961 gctcgcctct ttaggcgtcg agcccgagac gctgacggag gagcagatct cctaccagca
     1021 gagctggcac tagaggcccc ggcagagacc cgagcggaac ttgcggcgac tctcccgaac
     1081 cggccagcga aggacggcat gacaaaaaag accccggcgg ccaccggaac cggaggcggg
     1141 ctccggagcg cgctgctcgt ggtcggggtt gtcctcctgg cggccaacct gcgtccggcg
     1201 atcatcgcgg tggcgtctct tataggcgag atccggcccg acgccggact gtcgaatggg
     1261 acggcgggcc tgcttacgac gctgccgctc ctggcgttcg gcctgctctc tccggtcgcg
     1321 ccgcggctgg cccggcgctt cggcatggaa cgcgtgttgt ttgcggggct cgtcgtgctc
     1381 gcggcgggga tactgctgcg ttcgcagggc acggtcgcgg cgctgttcct cggcacggcg
     1441 acccttggag cggccatagc cgtcggtaat gttcttttgc ccgccgtcat aaagcgctcc
     1501 ttcccggacc gcgtcggcct gatgacgagc gtgtacgtta cagcgctggc cgtcagcgcg
     1561 gccctggcgg ccggtgtgag cctaccgatc tccgaccaac tcggcgccgg ctggaagacg
     1621 gctctggccg tatgggcgat cccggcgttt gtagcggcgg ccgtgtggct cacgcaactt
     1681 ggacgcggcc gtcaagtgga tggtcccgac ggaacatccg gcgcccccgg aaaggtcagt
     1741 ggcctgtggc gttcggcgct cgcctggcag gtcacgctgt tcatgggcct tcagtctctg
     1801 gcctactacg cggcgctggc gtggctaccg gagatcctgc gtgatggggg gatgggcgcg
     1861 gccggggcgg gctggatgct cgcgctcgcc cagacggtcg gtatcgccgc catgttcctg
     1921 gcgccggtcc tcgctggccg cacgccctca cagcggggcg tcgtcgtggc ttccgtggcg
     1981 ctgtcgggcg ccggagttct gggactgctc gtcggcggta tcgggctcgc tcccgtctgg
     2041 gtcgcgctgc tggggctcgg acagggagcg tgcttcagcc tggcgctcac cttcttcgcg
     2101 ctccgctccg ccgaccctgc gcacgcagcc gcgctctccg gcatggccca gtccgtcggt
     2161 tacctgctcg ccgccgccgg tccattcctg ttcggcatcc tccgcgacgc gacgcacgcc
     2221 tggactgttc ccttgatgct gctcctcacg atcgtggtgt gcctcctgat cacgggcctc
     2281 ggcgccgctc gcgacgctca ggttggttag ctgcgtacga cgaggacgcc gccgatcagg
     2341 atgaagacga tgccgaggac tttgacgagc ccgatctccg ccgccgccgg accgtagacg
     2401 ccgagccgat ctatgaccag tcccatgatt agctgcgcgg ctatcaccaa cgagagcgtc
     2461 tgggcgacgc cggtctgtcc ggccgcgagc gtaatggagg agacgatgaa cgccccgagg
     2521 atgccggagg cgatcgcgca ggtaaccgct ttaccggtaa gctccggtcg ggtcatgaac
     2581 gaggcgacga ggaagccgat cacccctgtc gtgaagccgg agatggcgac cagaccgccg
     2641 atccccaggg acttcatgcc cgttgtattg gcgacgacct ggaaaccgac cgcgacgccg
     2701 gcgaggaccg cgagaaccag cgcgggattc aattgcgctc acccctctct gttttgggcc
     2761 agcgaggtag tatagcagcg tgaggaccat aaccatccgt tacacggagt ttcgtgaatg
     2821 cccgaactgc gagggacggg gcgacgacgg ctccgcgtgg cggccgttcg agtgccgccg
     2881 ctgtaggggg agaggccggc tcattaccgg agtcgaggtc gaggactcgg tggaggggct
     2941 gctgccgcgc tcgcgctacg acttcaccgc gctgggagag gtctacctgc tgaacttcga
     3001 cgaagcggag ctggaggaga tagaggtcat tcgtccggag aacggttcgg gtccacgtgc
     3061 gccatcagcc ggagaccctg agtgaaggcg gccgctatgg agatcccgat gatgtcgaag
     3121 gtcgcccagc ggccgtaatc gggcctgctc accgcgtcgc cgtattcctt ccggtagccc
     3181 gcggggtcgc tgatcgccgc ccactggaac gccgccggga agagcgccca ctctataacc
     3241 gtggcgccga ccgtcatcac gactgccgcc aggacccccc accagccgcc ccggaacacc
     3301 agcgtcgccg ccagcaccag gtacacgatg aggcgggtca gaaggataag agtcccatag
     3361 ctgctccctt catacgcgct cctgaacgag aacatctcgg agccgaaccc gaagagcgcc
     3421 gccgcaaagg tgcatcccac cgtgaggata gccagggatc tcaggtctcg cttcatgggc
     3481 cgcattatat tcgtttccgc ccccggatag cgtcgcgcta gaggtccgtc agggcttcga
     3541 gcacggcttt cggggccagc cggttcttga atggcaccac ctcgccgtgg accgcttccg
     3601 agagcgtctc cttcgtggcg ctcaggttct cggaggctgc gagtagtacg cggtgtccgc
     3661 ggcggcgggc ctcccgaacg agggcgaccc gctcgatcag ggacctctcc gaccagaagc
     3721 ccaggatctc caggtaagcc cgttccccgg tgtcgctcgt cagcgcgaag tccgggacga
     3781 gcgccgcctt tagctctggc aggggaagta tctccgctcc ggcgtcgagc cgccatccgc
     3841 cggtatcttt ggcccgctcc caagccttca cgaaagcctc gcggacctcg tcgggttcgc
     3901 gggcctcgcg cggaccgagg tagtggctcc gcagcccgga ctccgagtcg agctccagga
     3961 cggcgtcgcg gcccttccac tcgacgttcg ccgagaggga ccagggagtg gtcaggagca
     4021 ggccgggcag gaacttcgcc agcctcagcc cgtacttgcg agtgccgccg aagatggaga
     4081 gcgggccgtc gaggtggagc cggtagccgc gggacgtggt ctccagccgg tagacgagcc
     4141 ccatgagctt tacgtagtgg aagacgagcc gggcgtccgc gccgggtccg aggtccacga
     4201 tcaggtcgcg ggcggagtag agaaccccct gggcctgggc gacgttgtag cgcagcacca
     4261 gggcttccgg ctctggacgg tcgaagacgg agagtagctg cgccgcctgc cggtcggcgt
     4321 acattacggc cgacgggtcc accagcgtag tctctataga cacttgcgag aggaggtcgt
     4381 cgcgggtcgt ggcggcgagc aggcccggct cggcggccgg aggttcgggg agggcggcgg
     4441 caagctcgaa gaggcgggtg cgtacggtgt acgggtcggc ttcagtgggt ggctcccacg
     4501 acgcgcgttc ttccaggagc ttcgccaggg cgcgcacgat cctgaacccc cgccgggcgt
     4561 cgaggcgcgg cccgagctcc tcttcccgct cggcgagcgc ggcctgcagc gcggctctcg
     4621 gctggccgag gtgcccggcg tagaggtcgc agagttcagc ggcgatttcg aggacgcgcc
     4681 ggtcggaggt cgagaggcgg tgcgggacga ccctgccgcg gctgacgcgg gccatgacgt
     4741 gttcggagcg cagcatcaga cgggtattcc gcgggtagac tcgcggcggc gccgggcggt
     4801 gaactcctcg ccggtcgcgg tcgtgaccag ctcgtagaga gtggcgcgct tgccctcctt
     4861 cgggcgcagg atgcgtccca gacgctggac gtactcgcgg tgagaggcgc tgccggccag
     4921 gatgatcgcg acgttcgcgt ccggcacgtc cacgccctcg ttcaggacgt ccgaggcgat
     4981 gatcgtgcgg tagcgcccct cccggaagcg gtccaggatc tcctttcgct ccctggccgg
     5041 cgtctcgaac gtgatgccgg ggatgaggaa ccgccgcgag agcgcgtaca cctcttccac
     5101 actctttgta aagacgatgc agcggtcgtc ccagtgccta cgcagcaggt tctccagcgt
     5161 cgcgcccttg cgcaccgctc cggcctgtat ctcccgccgc cgccgcgccg ccagcagcgc
     5221 ctcccgcccc cctgagtggc tggtcgcggc gacgaggatg aaacgctgcc agtcctcagg
     5281 cgagcggacg gagaggtgat gtttcttcag gaagtcccgg tagatagcct cggcctcggc
     5341 gtactcgtgg cgctcgtggg cggtcagctc caccgggatg cggatcaggt cgtagggagc
     5401 gaggtaggtg ccggcgaggt cttccggtga gagatggtag acgaccggac ctaccagttc
     5461 cggcagcagg tcgtggccgc catcgggacg ttcgggggtc gcggtgaggc cgagcgagta
     5521 aggggcgagg agcatcttcg agatcacgga gttcttcggg gcggggaggt ggtgtacctc
     5581 atcgtagacg gcgagggcga agcggtcgcc gtagcgttcg gcgtggatgt aggccgagtc
     5641 gtaggtcgtc accgtgagcg gcgtcacctc atggtacccg ccgccgagca gcccgaccgt
     5701 gaccgagtcc ccgaaggcgt cctgaaggat ggagtaccac tgctttagca gggccagcgt
     5761 cggcacgacg acgagggcgc tgcgccctac gcgctctatg gcccgcacgg cgacggcggt
     5821 cttcccggct cccgtcggga gcaccaccac gccgcgcagg tccgcccgcc gccaggcggc
     5881 gagagcgtcg cgctggtagg ggcggggttc catagcgacg cgggattcga gggagagctt
     5941 ctcgaaagcg gctgcgcggt cgcggtgggg cgtcgaggag ccgcgcaggg agagcgagac
     6001 ctcccgatac gcgctcgccg gggcgcgcca ctgccgggtg cggccgtccc agatgaaagg
     6061 ctccggcgga tggtcgccat cggggaggcc gctggcgatc agggtgccgg actcgtagaa
     6121 tagctcgatc acgctagaag gatacccgag cctcgcgccg ctctcagcgg tcgatggctc
     6181 gcctcatacg gctgaaggga gatcttgaaa catccaggca gaggaggatg ggccgcactc
     6241 gtgtgcggca tggtcgtggt gattggcgtt gtggccgcgc tgttggcggg ctgtggcggt
     6301 atgtccgggg atgcctcggg cacagacgcg aagtcttcct ccgcgcagca ggagaagaag
     6361 accgaagata agccggcggc cggggagatg agcgtgcgcg acatggtggg ccagatgttc
     6421 gtggtgagca tgggcggcac cgagccggac tactacatcg agaagatggt ccgggagcgg
     6481 aacatcgggg gggtcttgct cttcggctac aacatgaaga gcgaggagca ggtcaagtcg
     6541 ctcacggcct cattgcagaa gctctccatg aagacgaagc ctgcgatacc gctttttatc
     6601 ggggtggatc aggagggagg acggatagcg agcgcgccgt gggtagcgcc gcagccgtcg
     6661 gcggcagaga tcgggcagcg cggcgacccg gtcgaggcgc acaacgtggc cgagaagatg
     6721 gggaccgaac tgttgcgggc cggggtcaac acggacttcg cgccggtagt ggacactgga
     6781 ttcggggccg ccatagggga ccgctcttac ggtgaggacc cgctgctcgt gtcgaagatg
     6841 ggggccgcgg cggtgaaggg cttcgaggcg gcgggcgtcg tcgcggccgc caagcacttc
     6901 ccgaaccacg gaccggccac ctccgactcg cacgtgagtc tgccggttgt ggaccatgac
     6961 atggcgacga tccgctccta cgacctgccg ccgtttcagg cggccataaa ggtcggggtg
     7021 ccgatggtga tggcggggca cctcgtctat ccggccctgg acccggataa cccggcgagc
     7081 ctctcgccga aagctatagg gatgctacgc ggcgatctgg gcttcgatgg cgtggtcatc
     7141 accgacgacc tggcgatgaa gggggcgaac ggcggcgggc cgcccgcccg cgcggcggtg
     7201 gaggccgtca aggcgggggc ggacatgctc attatctcca gtccgccgca agagcaggcc
     7261 gacgcgtatg acgccgtggt cgaagccgtc caatccgggg agataccgga aaaacagatc
     7321 cgggcctccg tcgctcgcgt actgaaagta aaggaaaaat actcgctcta cggagagaaa
     7381 agccgatagg ggtgcaaact ctcaagttag ggtagaatgc cgtaggccat taggggtggc
     7441 ctctccccgg caggggatac tgtgggaact acgggcgcga acgcgctcga aaaatagaag
     7501 aaggaggcga cagatggccg aagttacgct tgaggacgta accaagatct atggcgaaga
     7561 tgtggtggcg gtcgataagt tcaacctcga catcaccgac ggggagttca tcgtcttcgt
     7621 tgggccctcc ggctgtggaa agtctacggc tctgaggatg atcgccggtc tcgaagacat
     7681 atctcgcggc aagatattca tcggggacaa cgtggtcaac gaccttcctc cccgcgagcg
     7741 cgacatcgcg atggtcttcc agaactacgc gctctatccg cacatgaacg tgcgcgagaa
     7801 catgggcttc gccctcaagc tgaggaagat ggacaagaac gagatcaacc gccgcgtgga
     7861 tgaggcggcc aggatcctct ccatcgagcg tttcctggac cgtaagccga aagcgctttc
     7921 gggcggtcag cgtcagcggg tcgccctcgg acgagccata gtccgggagc cgaaggcgtt
     7981 cctgatggac gagccgctct cgaacctcga cgccaagctg cgggtggaga tgcgcacttc
     8041 gatctccaag ctgcacaacc gcatcggcgt gaccaccatc tacgtcaccc acgaccagac
     8101 cgaggcgctg acgatggccg acaggatcgt ggttctcaag gacgggaagg tgcagcagat
     8161 cgactccccg caggtgatgt acgagaaccc gaacaacata ttcgtggcgg gctttatcgg
     8221 ctctccgtcc atgaacttca tccgggcgca tatcgagcag gacaatggcg gctacgctgt
     8281 caagttcggt cctaccagcc tcccgatctc gcgtgaagct gctggagagt caaaagagcg
     8341 caggggccgg gacatcgggg agtacgtggg taaggaagta gtgctcggta tacggccgga
     8401 gcacatcgag gacgcctcgg tcgaggaggc ggcggccctg atcaacgccg accacacgac
     8461 cagcatggag gtcgagccgc aggtcataga gagtatgggg agcgagaagt acctctactt
     8521 cgagatcgac cgaagccaga ctattcacct cgatagcgtc tcggatatga cggatgactc
     8581 cgcggcggat gaagagagcg gcggtacctc cgggacttcg ggctcggtcg ccgggggcga
     8641 gatgatgacg gcccgtatct cggcggcgag caaggcgagg ctcggtgaga agatggagtt
     8701 ggtcatcgac gccagcaaga tgaggctgtt cgacccggag acggaagaag ccatacttta
     8761 aagaggtctt acagtaccgg gcgcggaatc gtccgagccc ggttttgacc tatggagata
     8821 acctctaggt ggaaagggac gcaagcatga cggagaggca agaacggaga ccggagacgc
     8881 tatccgggcg caggatcaac cgccgggact tcctgaagat cggcggcgcg ggtcttgcgg
     8941 gggcggctct gttgggcgtg gcgggctgtg gcggcggtgg cggaggcgga agctcgggcg
     9001 ggtccggcga catcgttttc gccatgggtc ccgatacttc cggcagcctc cagaagttga
     9061 tcgacaagtt caacaaggag aacaagggaa agtactcggt caagtaccgg aagatgccga
     9121 cggacacggg acagttcttc gacaagatcc ggactcagtt ccagtctggc ggcggcgaca
     9181 tagacgttat cggcggagac gtgatctggc cggcccagtt cgcggctaac ggttggatct
     9241 ccgatgtctc ggataagctc tcggatagcg agcggagcaa gttcctggat ggtcctatag
     9301 cgtcgatgac ataccagggc aagctctacg gaataccgtg gtacacggac gcggggatgc
     9361 tctactaccg caaggacctc ttgcagaaga gcgggttcaa cgaggcgccg aagacctggg
     9421 acgagctgaa gtccatggcg aagaaggttg tgcaggattc cggcaccaag gccggtttcg
     9481 tgtggcaggg ctcgcagtac gagggcggcg tggtcgacgg cctggagtac atcaacagct
     9541 acggcggcaa ggtcctggac ccgagcgacg ccagcaaggt cgtcatagac agcccggatt
     9601 cggtgaaggg cctggctatg gccgccagca tgatcccgga tggggtctcg cctcaggccg
     9661 tgacgaacta caccgagcag gagtcgcaga cggcgttcct caacggcgaa gcggtcttct
     9721 gccggaactg gccgtacatg tatgcgctcg tgggcaaccc ggacgagtcg aagatcaaga
     9781 cggatcaggt cagcgttgcg ccgcttccgg cgggctccgc tggaagtgtc agcggactgg
     9841 gcggatggaa cttctacatc aacgccgctt cgcagaagca ggacgcggcc atcgcgttcg
     9901 ccaagttcat ggccgatccc gaacagcaga agtaccgggc gctcaacgga tcgttccttc
     9961 cgacgctcaa gtccctatac gatgacaagg agatcctcga cgcggttccg gttatcgcgc
    10021 tcgccggcga agctctggcg aacacggtcc cgcggccggt ctccccggct tactcggata
    10081 tgtcgctcaa gatggccgag cagttcaaca aggccttcaa gggcgacgtc tcgccggaag
    10141 atgccctgaa gacgctccag accgacctgc agcagatcgc cgatcaggca tcgtagtcca
    10201 ggcagatctc cggcggggat tctctccccg ccggaggctt cgaaggagag gtgagtagtg
    10261 acgactgtat cgagagagaa gaagagggga tggttaaagg aggagttcgg taaccccgag
    10321 cggcgcacgg cctactacat ggtcttgccg gccctgctta ttatcctcct catagctttc
    10381 tatccggtga tcaattcgat gtggttgagc gtacaccagt cgacgattac tcagacgggg
    10441 cctttcaacg ggttcgagaa ctacgccggt atctttacga acacggactt cctgggcggt
    10501 ctgttcaaca cggtcgtctt tacggtgatt agcgtggcct tcgagttcgt aatcgggctc
    10561 ggcatagcgc tggcgatcaa ccgggcattc cggggtcggg gactggtgag ggcggcggtc
    10621 ctggtgccgt gggccttccc gacggtcatc tcggcggcca tgtggcggct gatgttccag
    10681 gatcaggtcg gcgtcatcaa taccgtggcg aacggtgtag gaattatcag cgagccgatc
    10741 ctctccgata ggacgcttct cttgatcgcg tccatcctgg tggacgtgtg gaagacgacg
    10801 ccgttcatgg cgctcttgct gctggcgggt ctccagacga taccggccga cgtgtacgag
    10861 gcggcgaggg tggacggagc caatgtcatg cagcgcttct tccagataac gatgccgctg
    10921 ctcaagccga cgatcctggt cgctgtgctc ttccggacgc tcgactccta ccgggtctat
    10981 gacctcttct gggtgatgag caaccgtcag ctcgagtccc tctcgacctt cgtttacaag
    11041 ggagtcagga tcagtcagtt acagttcgcg cagggtaacg cggctgcggt cttcatcttt
    11101 atcaccgcgt tcctcttggc gctgttcttt atctacggtc tcggtatgca gacctcgacg
    11161 gaggagtaga gatggctagc gcaacggcga ccggcggctc caggagcggt tccgccaaca
    11221 tgattctctt ctacgtgttc ctcttgctgt tcgtcctggt gagcctcttc ccgctgctgt
    11281 gggtcttcaa gatgagcatc gtcaccaaga gcgacctgtt ccagtcgccg gtgactaccc
    11341 tggtggcgcc tccgatctcc gataactacg gcacgatctt cagcaaccct gacttccgga
    11401 gggcgctcct gaatagctcg gtcatcgccg gggtgacgac gattatctgc ctgttcttcg
    11461 gcgcgatctg cgcctacgcg atagcgaggc tcaggttccg gttcaagagc gcggtcatga
    11521 cgacgatcct ggccctgagc ttcttcccgg cggtggcgat catcgctccg ctcttcgtcc
    11581 tgttcagcaa caccggcctg atcaacacgt actggtccgt gatcgttacc gatacggtct
    11641 tcgcgttgcc gctgactatc tggatactgg tggccttctt caaggagctg ccgaaggacc
    11701 tggaggaggc ggcgaaggtc gacggggcga ccacgatcca ggcgttcagg aaggtcatag
    11761 tcccgctggc ggcgccgggc gtgttcacga cggcgatcct gacattcatc ttcgcgtgga
    11821 acgagttcct cttcgccaac acgttcctgt tcgacgccag cacgcagccg gttacggtcg
    11881 cgatcccgaa cttcgccacg gtctatacgg tggactacgg ggcgcaggcc gcagcagcgg
    11941 tggtggtgac gatcccactg gttatactgg tgctgttgtt ccagcgccgg atcgtctcgg
    12001 gcctgaccgc cggagcagtc aagggctaaa tttccggcgg gccggacggc atcatgtccg
    12061 aaccggcagg accggactag aagggcgctt cactgaataa ggagaagttc tgggcgcggg
    12121 agatcgagcc tggcatgagc ctgcgctcga ccttcctcgc caccgactgt gttgtccgca
    12181 acgactctcg cggggcgccc tacctctccc tgaagctggt ggaccggacg ggctccatag
    12241 acgcccggat gtggggactg ccgttcgagc ttcgcagcgg cttctccggt cccgaatacg
    12301 tccaggtcga gggtaacgcc cacgagtacc ggggcaccct ccaggtgaag atagaccggc
    12361 tcaaggtgct gggccgcgag gagatctccg aagaggacta cctgcccgcg accgagaggg
    12421 accgccaggc gctcgccgcc gagttggaga tgtgtggccg cgaattggag gacgagcacc
    12481 tccggaccct cttcgagaag atagtcgccg atgaggattt ctgggaagcg ttctgcgccg
    12541 ccccggccgc caagggcatg caccacgcgc gcataggggg cttgctagaa cactcggtgc
    12601 agtgcatgag gatcgcgcgc ggaatctccg acctttaccc ggtcaagcgc gacctcctga
    12661 tcttcggggc gatctttcac gacgcgggca agacaaaaga gctgtcgtgg ggcggaggag
    12721 ggttcgcgta cacgacggag gggcggctcg aaggccacgt cgttctggga gagcggctga
    12781 tgtcgtcata catcgctgag ataccggagt tcccggagga gctgaagctg caaatctcgc
    12841 atatcctgct cgcgcaccag ggagagatag agtacggctc gcccacccag ccgaagacgc
    12901 tcgaagcact tctggtccac ctgatagata acctggacgc ccgcgcggag atgttccttc
    12961 agagcaccga gaacgtcagc gccggcggat ggagccatca cgagaacccg ctgaagcgcg
    13021 cgctatatgt gccgcaggag aaggagatag aaggggagta aggtctcaga gaccgctaga
    13081 ggtctcgcct gccgtcctta ccatctttac tatcgtcgtc ctcgtcttca tcgtcgtgct
    13141 cggcggtgag ctcgtccttg aactcgtcta tcgagcggcg ggcctctgcg acgaacttac
    13201 cgaggtcgcg agccatctgc gggagcttgc tgggaccaaa gatcaacagg gccagcagcc
    13261 cgatcattac catctcctgg ggacctactc cgaacacgct tatatcttaa accccgaagg
    13321 acatggcgac aatgaatggt tgtccgaagg ttgtctatgt gacggccctc gcggcccagc
    13381 gtatatactc agggcatgat gatctcagca atttcttacg ataggaccgt accatgatct
    13441 cctgggcggg tgtcagaacg atagccgttg cgctggcctc gcggtcggag agggtcccgg
    13501 cgggtgactt cgattatccc ggcgcggtcg aacgcacgct cgacccgctc tccaggttca
    13561 ccggcatctc cataccgccc tcgggacgtc agctccgcat cgcggaccgg gcggagtgga
    13621 tcgacttcaa catcgaaggc ttcggcgcgt taatggagcc ggtactgaag cgggcctcgg
    13681 ccggcgccga cagtgttacg tggttcctgg gaggcgtaac gctcacggcg caggtcgggt
    13741 tgctgctggg gttcctatcg gcgcgggtgc tcggccagta cgataccggc cctctcctgg
    13801 tgcgggagcg gagcggagag ccgggtaagg tcttcttcct tgacggaaac atcgtctcgg
    13861 cggcgggccg gatcggggtg ccggtagacg gcctcaggct ctggatcgtg ctgcacgaga
    13921 tgacgcacgc cctgcagttc gagggctacc cgtggctcag gagctaccta ggagacctgt
    13981 tggagagcct gatctcgccg ctcgccgaac gcctcggggc gcgggagact ctccggcgca
    14041 tctcccgcaa cctctcgacc ggcggacgct ccgtggagtt gatgatgagc gtcccgcagc
    14101 gcgaggcgtt cgaccgcatg caggcggcca tgtccgtcat cgagggctac tccgactacg
    14161 tgatgcacca cgtcggacgc ggcctcgtcc ccaactacga gaacctcagc cgccgcatgg
    14221 cgcgcagccg ggcccaccgg ccgccgctgg agaccgccat cttccgtata accggcatgg
    14281 acatgaagct ggagcagtat cgcctcggcg aagccttcgc cgacgccgtg gtccggcgcc
    14341 agggcatgca gggtctcaac cgcgtctggg agcgccccga gaacctgccc actctgcaag
    14401 aggtccggga ccccggcctc tggatgtcga ggatggaaac cgcctgagat gtccgagatg
    14461 tccggtgagg agcggcgtcc cgcccagact gtaagctacg agttcgccac ggagaacgcc
    14521 gcgcgcgacg ccgcccggga gctggaccgg cgtttcgacc agaaggaact cctcgcgctt
    14581 cgcatctacc gcgtgcgctg gaacggcaac tacctcgtcg aagcctcgtt cgaccccgga
    14641 gcgcaggaca cccggctgcg agatgcgcgg gcgctgctcg gagagtccgg ccggccggtc
    14701 caccctgacg acctcgaaga ctacaagcgc gccacgaggg agggcggcgg gctatcgggg
    14761 tggtttcgca gcttcttcgg ttaggggcgg gctacgcgcg gcggcatgcc aagacggcgt
    14821 ctggagtacc ggatggccga gagtggcata ctctcggagg cggatcgcgt cgagttcatc
    14881 gagggtgagg tcgttgaggt ctttaagacg ctgctcgact acgatgtcga ggtcaaggtc
    14941 ccgctctatg cgcgggctcg cataccggag gtatggatcg taggtctgtt cgccggggag
    15001 atctatcaga gtgtcgaacg gctgagacgc gaagagctta cttcgagcgc agtgcccggt
    15061 cttaccgtac agacaggcga cattttcgga tagaggctag tccatgcgtt gttcgaggcg
    15121 tgtgatctgc tctcgcagct cctcgatctc gtgctccaga tacttctcgc ggccccggac
    15181 tctggtgtag ccggactcga tggccgtgcc gagatggtgg acggtccgta ctgttgcccg
    15241 tccgacgtag cgggccgagc ccccgagtcc gccgagggcc gactggccgg ccgagcgggc
    15301 cgtgggggtc gcggcgaagg agcccacggc gaagccgccc gccgcgccta ccagtacccc
    15361 gaaaacgaac tttgccgaac cctcgcgcaa ccgaacgcac catccttccg caccctcgcg
    15421 tgacggcgcc agtttagcat agcctcgcgg gataccggct ctcggcggtc ttcgagcggg
    15481 ttatactggc cccaggtgtt ggagacgggc ggacacgagc gactcaggaa cctgccggcg
    15541 gttgacgccg tgctgcgtag cccgcacgcg ggcgcgctcg ctcaccgcca cggccgcgcc
    15601 gccctgaccg ccgccgtccg cgagtctctc gcctcgatgc ggcgctctat actggccggg
    15661 gaactgccgg aggtgtccga ggaacgggtg ctttcgtccg ccgcccggat gctctcgact
    15721 cgcggtctgc ggccggtggt caacgcgacc ggcgtgatcc tccataccaa cctggggcgc
    15781 tcggtcctct ccgaacgggc ggtggaagcc gtcaccgagg cggcgcgaag ctactccaac
    15841 ctggagtacg acctgacctc cgggagccgg ggatcccgct acgatcatgc cgttccgctc
    15901 ctacgggcgc tgacgggcgc tgaagacgcc ctggtggtta acaactgcgc gggagctacc
    15961 ctgctcgcgc tcgcctccct ggtcggtgag acggggaacg gagaggcgcc ggaggtaatc
    16021 gtctcgcggg gtcagctcat cgagatcggg ggcgggttcc gtatcccgga ggtgctggcg
    16081 ctctccggcg cccggctgcg cgaggtgggc actacgaacc gcacccggct cgccgactac
    16141 gagggcgcca tcgatgaaaa cacgcgcgcc gtactctggg tgcatccgag caacttcgcc
    16201 accgttggct ttacggagtc cgtcggagtg gcggagctgg cgaggctgga agttcccgtg
    16261 atcgccgacg tggggagcgg ggcgatgttt gcgatgggtg acgaacctcg ggtacaggag
    16321 tcgctccggg acggggccgc gctggcgatc ttctcgggcg acaagctcct cggcgggccg
    16381 caggccggaa tcgcggtggg acgctccgga ctgatctcgg cgatgcgccg ccacccgttg
    16441 gcccgcgccc tgcgcgctga caagctctgt ctcgccgccc tctacgccac cctgaccgcc
    16501 tacctggaag gcacggccac ggaggaggtc ccgaccgcgc ggatgctcca cgcctccacc
    16561 agcgaggtcg aggtgcgggc caccgcgctt ggagcggcgc tcctgcgggc cgcgcccgag
    16621 atggagatag atgtcgcgcc gtccgttgcc cgcagcggcg gtggcaccat gcccgtctac
    16681 gagataccgt ccttcgccgt ccgtatcgcg ggcgacgcgg actcgctggc tgcccggctt
    16741 cgggcggcgg acccgccggt gatcgggcgc gtccatgagg gacgggtgtg gcttgacgtt
    16801 cgcactttgc tgccggggga cgaggaggcc gtcgaaagag cggtgagagg actccgtggc
    16861 tgaccggccc atcgcgctca ccatcgggac ggcgggacat gtggatcacg gcaagaccac
    16921 gctggtacgc tacatgaccg gcacggatac cgaccggctg gaggaggagc accgccgggg
    16981 catctccatc gtgccgggat acgcggagct tgacttaccc ggaggacgcc gggcgagcct
    17041 cgtggacgtg ccgggacacg agcggttcgt caagaacatg gtcgccggct cgacgggcgt
    17101 ggacgccttt ttgctcgttg tcgccgccga cgacggcgtc atgccccaga cccgcgagca
    17161 tctggacatc ctgcgcgtcc tcggcgtcga gcgcggcgtg gtcgccctta ccaaagtgga
    17221 cgccgtggat gaggagatgg cggacctcgc ggagctggac gtggacgaac tgttggaatc
    17281 ggcggggggc gaggctccgg tggtgcgtgt cagcggcgtt accggcgagg gcgtggatga
    17341 gttgctgcaa gcgctcgacg agctcgccgc gacaccgtcc ggtcggggaa gtagcgatct
    17401 ggcgcggctt ccggtagacc gggcgttcgt gctcaagggg atcggggtcg tggtaacggg
    17461 gacactgtgg tccggggaga tccacatcgg cgacactctc tacgcttccg gccaccgtcc
    17521 cagggtgcgc ggcatccaga accacggcca cccggccaaa gtcgctcatc ccggcgcgcg
    17581 caccgccctg gacctcgccg gcgtggagcc ctcgcagata caggccggag acgtgctact
    17641 ctcgcggtcg ttgtcgccga cgcgcgacct ggacgtgcgt atccggctcc tcgaaacagc
    17701 gaggcccctg gcccacggcg tccgggtgcg gctgcaccac ggcacccgcg acacgaacgc
    17761 ccgcgtccgg ctggtggacc ggggtgagat cggtccgggc gacagcgccc tggcccggct
    17821 gaagctggag gagccgctag tggcgctctc cggcgaccgc ttcgtcctgc gctcgatggc
    17881 gccccagatc accatcggcg gcggcacggt gctcgacgcg gctcccacgg gacgccgacc
    17941 ggaccccgtc tggctggaag ccctcgaagg cggcgagacc gagaggttgg tgtctatggc
    18001 gctctcccgc ccgccccgcg agggcatgac ggccggagac ctttcgctgg tcctcggaat
    18061 ccctccggcc gaagtactgg cagccgcgca cagcaacgtg gaggtggagg aactcggaga
    18121 gcttctcgtc cctgccgaag aggtggccgc ggcgcgccgg cggctgctgc aagcgctgcg
    18181 gttgcgggcg gaggagcgtc ccgagaaccc ggagttgacc gtcgcggagg cccgcaccgc
    18241 tacggggctc ggcgcggggg cggcggatgc gctgctcgcg gagatggcgg ctgagacgat
    18301 ccggataaca gattccgggg tgagcctgcc ggatgcgaac gaggctccgc cggaactcga
    18361 gaacgaggct cgggagcttt tgacagagct taaaggggct ggggtggagc cgccggcgct
    18421 ggagcctacg cccgcgctgc ggctgctcgt gaagcgcggg gaggttgtgg atctcggcgg
    18481 caaaatctat ggctcgcggg aggccgcgga ggctgtcatc gaggagatca aggcccattg
    18541 ccgcgaggat ggcgacatct cactggcagg actgcgggac cgcctgaaga ccagccgcaa
    18601 gtacgcccag gcctggctag aatacgcgga taccgccgga gttacccgca ggatcgggga
    18661 tgttcgcgtg ctgacccggc gctaccggca ggcgatggat taaaatagcc gcgcactccg
    18721 gacactggaa tgcgtgggcc tggtggcccg gccggtcttc aaaaccggtt tgcgggcgca
    18781 cggcgtccgt ggcggttcga ttcccccgca ttccgccgcc ggacggtact caggacggag
    18841 cccgatgccg gacacctttg aagtgatcac gtataacgcc tggtgcgagc gcggcgggcg
    18901 cgacccgctg ctcgacgacg cgctggagga tggggccatc gtctgtctgc aggaggtgaa
    18961 gccgcggcgc gccctggaac tgaagcggcg cttcgggaaa agagcgttcg tctcgcccgg
    19021 ccggcacggg cttcagtaca tcgccctcgt gctccctcgg gacgcccgtt tcgttcggcg
    19081 gcgcacggtc cagctaaacg gtatcgtcgg tctcgtgccg aggcgctggt cgctgcggcg
    19141 tggggcggcg atgcgtcgcg ccgggcggcc cggttggaga gactgtttcg agccgcgggt
    19201 ggcgcaggag gccgacgtgg agtggagaga ccggcgggtg cgggtcgtga acactcacgt
    19261 gccctatgcg ccgctccttc gtgacccttg cctggggatg ctcgcggcgc tgaccggcga
    19321 gggcgatgcc atcctggccg gcgacctcaa cgcgccggcg gagtcgctgc acttgaacga
    19381 cctggtcctg gccaccggtc ttcggacgac aggtatgggg aaggccaccc acggacggca
    19441 caaaatagat cacgtgctcg ttcgggggcc gtttcgagag gccgggtacc ggacgaggga
    19501 agggaagagc gatcaccgcc tcctgaaggt ggtgctggag gtgaatgagt agtggtggac
    19561 ttttcgcaca tagaccgcga aggatcggcg cgcatggtgg acgtggggga gaaggcggtg
    19621 atccggcgca ccgcaatagc gggtgggcac gtcctcatgg cgccggagac gatagagcta
    19681 ctgcgtgaca gggcattgcc gaagggagac gcgctcaaca ccgccaggat cgcgggcgtc
    19741 atggccgcaa agcgcacccc ggacctgatt cccctgtgcc acggcctgaa cctgacctcc
    19801 atcgacgttg acttcgaggt cgccggggac cggatcgaga tctccgccac cgcccgcgcc
    19861 agcgaccgca ccggcgtgga gatggaggcc ctcaccgccg tcagcgtcgc cgccctgacc
    19921 atctatgaca tgtgcaaggc cgtggacaag aacatgacca tcggggaggt gcgcctgatc
    19981 gagaagacca aggagagcat cccggagtga agccttatac ggtctaccgg tgaggagcat
    20041 gcaacacttt gccagcaaat gctagtcaga ggtaacgagc ggccggttgt gctagaattc
    20101 cgggccaatc cgggcttgta tagctcttgt taggagagag agatcgaggg cgaaaatact
    20161 gtcctttacc ccatatttct ggacctctcc gggagacgct gcgtggtcgt cggcggggga
    20221 gcggttgcga accgcaaggc ccgcaagctg ctccaggcgc gcgcggaggt ggtcgtgatc
    20281 tcgccggaag tccaaccgga actcgcgagc atggcccgtg aggttcgccg ccgtccgtat
    20341 caaatgggag acctggaagg cgcctatctc gcgttcaccg cgacggacga ccgcgaagtc
    20401 aacgccgccg tcgcgcggga agcgcgcgaa cggagcatcc cgatcaacgt cgcggacaac
    20461 ccggcggagg gagactttgc gctgccctcg acgctgcggc gcggcaggct tcaggtatcg
    20521 gtctcgaccg gcggcgcctc cccgacgctg gcgcgccgga taaaggacga gctggagaag
    20581 ctgttcgggc cggagtggtc cgggctggtg gaggagttgg agcgcggccg gaggcgcgga
    20641 gagaaggccg aggagcggct cgaaggcgag gtgagccggt gcttatcgca gttgcgggga
    20701 tgagccaccg ctcggcgccg gtcgaggttc gggagcgggt agctttcgct ccgtgcacgg
    20761 cgcgggactt cctgcggcgg ctaaaggacg acggcgtcgt ctcggaggcg gtcttgatct
    20821 cgacctgcaa ccgtaccgaa ctctacgccg tggtcgagga cgaagtcgcg cgcgagaaga
    20881 tgctcgacgc gttggcggaa gaacgggatg tagaccgcgc gtcgctcgat gacgacacgt
    20941 actggctcac tgacgccgag gccgtgcgcc acctctaccg cgtcgccagc tcgctcgact
    21001 cgatggtcgt gggcgaggcg cagatactcg gtcaggtccg ggacgcctac cgcattgcta
    21061 ccgaggagca gtgcgccgga ccgatcctga accgcctctt ccacacctct ctccgggtgg
    21121 gaaagaacgt ccgcacccag accggtatcg gagacagttc catctcggtg ccgcacgtag
    21181 cggtgaagct cgccgccgag gtcttcggga ctctcgccgg ccggagagcg ctggtactcg
    21241 gcgccggcga catgagcgag ctggtcataa agcacctcaa gagccgcggg gtggcggaca
    21301 tcaggatcgc caaccgcacc ccggagcgcg cccggctgct cgcggagcgg atcggaggac
    21361 gggcggtcgc atttgacgcg ctggccgagc agttggcgga ggtagacgtg gtcgtaagct
    21421 ccaccggatc gggagattgg gtcgtcgggg cggacctcgt ctcggacgct ctcgacggac
    21481 gtgaggagcc gctgttcttc gtggacatcg ccgtgccgcg ggacgtagac ccggcggtgc
    21541 aggcgttgga gcgcgcctac gtctacgata tagacgacct tcagtccgtt gtggactaca
    21601 acgccgaggg acggggcgcc gcggcggaga gcggggaagc catgatctcc ccggccgtct
    21661 tcgagtacat gagctggctc tccaccttgc acgtcgtgcc cctgatccag gaactccgta
    21721 gcggggccga gcgtatccgg cgtcatgaat tgtcccgcgc gctcaagaag atggacctct
    21781 cgccggaaga cgccgcgtcg gtggagcgtc tgagtcactc tctcgtaaac aagctgcttc
    21841 acggccctat agcggagtta aaggcccttg ctgagtccgg cgctcccctg gacagcgccg
    21901 aggtccgcca gcgcctgctc gcgctggaga gcatcagcgt ggagctgcac cgctccggcc
    21961 ctaccgcccc atgactcctg gacagtccgt agtcctcggg acgcggggct cgaagctcgc
    22021 gctcacccag gcagaatcct gcgcccgtag gctgcgcgac gccggcctcg atgtggaagt
    22081 ccgcgccata aagacgacca gcgaacagcg cccggagacg cccttctccg taatagacca
    22141 gcgcgatgtc ttcacccgcc agctcgacga agccctgctc gccgctgaga tagacctggc
    22201 tgttcactcg ctaaaagacg tgcccaccac gctcagagaa ggaatctccc tggccgcgat
    22261 ctccgagcgg cacgatccct ccgacgctct catatccgag gagcgctatg acctcgacac
    22321 gctgcccgaa ggcgcggtcg ttgcgacatc cagcttgcgg cggcgcgcac aggtcctcaa
    22381 ccgccggccc gatgtgagca tcgtggagat ccgggggaac gttgacacga gggtccgcaa
    22441 gctgcgcgag ggcgcggcgg atgccctcat cctcgcccgc gcgggcctcg acaggatcgg
    22501 gctagacgcc ccgcacaccg tcataccgac cgaagttatg ctccccgccg tcggacaggg
    22561 cgctctcgca gtggccgcgc tcccggatca cccactctac ggacatatcc gcgagcacct
    22621 gaacgacgag attacggagc gcgcgacgac tgccgaacga gcccttttac ggacgctgga
    22681 gggtggatgc cgcgtccccg tgggagcgct aggagccgta cgcggcgatg gcgtctacct
    22741 gcgcggactg gtggcgtcca tagatggggc tctcgtatac cggggtgagg ccgaaggcga
    22801 agaccccgac gaggtcggag aacggctggc gcgggacctg ctggagcaag gggctgcggt
    22861 ggtgctcggc gaggtgcggg aggtgaggag ttgacggtct atctggtggg cagcggaccg
    22921 ggagaccccg gcctctttac cgtcaagggc gtccggtgca tgcgggaggc agacgttgtc
    22981 gtctacgacc ggctcgcccc ggagagcctg ctctcgcacg cgaggccgga tgcggagcgc
    23041 atctacgtcg gcaagaagcc cggcaacccg acgatgacgc aggacgagat caacgctcta
    23101 ctcgtcgaac tgggtcgcgc cggcaagaac gtcgtgcgcc tgaaaggcgg cgacccctat
    23161 atcttcggcc gcggcggaga agaagcgctc gcgctcatcg acgccggtat ccccttcgag
    23221 gtcattcctg gcgtaacgag cggcgtggct gccccggcct acgcgggcat cccggtaacg
    23281 caccggggag tctcgaccag cgtggccttc ataaccggtc acgaagaccc tacgaagggc
    23341 cgctcggacg tggactggcg gaagatcgcc aacggcgccg atacgctggt cctatacatg
    23401 ggcgtcggac ggctggcgga gatctccgcc gccctcatct ccgccggaaa gagtccggat
    23461 acgcccgtcg cctgcgtccg gtgggggacc cgcccggacc aggagaccgt taccggcacg
    23521 ctgaaggata ttgccgacct ggtcgcagaa gcgaacctca agccgcc
//
LOCUS       DASSMB010000055         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_266780, whole genome shotgun sequence.
ACCESSION   DASSMB010000055 DASSMB010000000
VERSION     DASSMB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266780"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VFJ72_03800"
     CDS             <1..1247
                     /locus_tag="VFJ72_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HET7478626.1"
                     /translation="HLGGARTALFNYLFARHHGGTILLRVEDTDAARSTREFEKTQLE
                     DIDWLGLSFDEGPYRQSERGDYYERAAERLAGAGLTYEAEDEVGRRALYLRPSERSGS
                     FRDELRGEVRFSRMEDFVIRKSDGTPSYNFAAVVDDLQMSITHVIRGEEHLSNTGRQA
                     VLYRALGEKEPEFVHLGVILGPDGKKLSKRHGAASVADYRDEGYLPEALVNYLALLGW
                     NHPAGREEFDGLDDLAREWDPSRLGASSATFDPDRLLHVNGRHIRALPVAQLRERVEP
                     FLEEPLPDGRGLISVEAIRDEMRTLSDAPRLLREILGPADPADFAGQLPESSDTVLAY
                     VLEALAKRSIEDIESTRSFVADLRSWAKARGIKTRDLLHPLRLALTGRDRGPEMAYLF
                     AVLGPEEARSRIERAREARLRA"
     gene            1244..2659
                     /gene="cysS"
                     /locus_tag="VFJ72_03805"
     CDS             1244..2659
                     /gene="cysS"
                     /locus_tag="VFJ72_03805"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7478627.1"
                     /translation="MSVRVRDTLSGRMVEVGQSGKVGIYVCGPTVYNHIHIGNARAPL
                     FWDVVVRYLKSRGYYVTFVQNITDVEDKIIGKANEEGVSWKEVVDRYTDSFHERLESL
                     GVGLPDVEPRATEHIPEMIHLIADLISSGHAYAPGNGDVYYAVESFPPYGALSKQRPA
                     EMRETEKSATGYKKSPLDFALWKASKPGEPSWESPWGAGRPGWHIECSAMVEKHLPDG
                     ADIHGGGTDLRFPHHENELAQSAAAHPDHTFVRAWAHHGMVNFADTGKMAKSLGNILD
                     VEQAVERHGRNALRMWLLQSHYSQPIDYSEEILEEKRRSYERLVRLYRQLSASTDDSE
                     LSERLAAELRERFDAAMRDDFNTPEAVAALFEVANRAGREVSARPEAAREFKALAAAI
                     AAVLSIFGFDLSEELSAEIDGVRIRYEEDPGEELLRRVAGRERARKERDWATADRLRD
                     EIQAAGWSVEDTSEGPILSRP"
     gene            2660..3364
                     /gene="rlmB"
                     /locus_tag="VFJ72_03810"
     CDS             2660..3364
                     /gene="rlmB"
                     /locus_tag="VFJ72_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET7478628.1"
                     /translation="MKPEVIYGIRPVVEALRSVRRRVFEVLDAVGDAEVEKAARTRGV
                     AVKRASRQQIEDLAHGGVHQGVAARVEPYSYSGLDEILTAPEPLVLILDGVTDPHNLG
                     AVLRVADGAGASGVVIPKDRAVGVTASAVKASAGASEHVRVARETNLRRAIDRMKEAG
                     VWVYAAGMDGTPYTRLDLAGPVAFVLGSEGRGVRRLVREGCDGAVSIPMLGAVESLNV
                     SVATAVLMYEARRQRG"
     gene            3366..3872
                     /locus_tag="VFJ72_03815"
     CDS             3366..3872
                     /locus_tag="VFJ72_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7478629.1"
                     /translation="MASYLILDAYNIIRALDRYGERNTGGFDESREMLINDALKAAGW
                     TGRVIIVVFDAHRSPEPRRDESRAGGAVRVIYSGAEESADDVIERLLGTLDGAATVYT
                     ADFALQRTALARGSTRATPSEFQELLDELPLLMSSPSKPRRVRVGDYLSSDTLRALEE
                     VRRRAERR"
     gene            3925..3996
                     /locus_tag="VFJ72_03820"
     tRNA            3925..3996
                     /locus_tag="VFJ72_03820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3957..3959,aa:Thr,seq:tgt)
     gene            4110..4191
                     /locus_tag="VFJ72_03825"
     tRNA            4110..4191
                     /locus_tag="VFJ72_03825"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4144..4146,aa:Tyr,seq:gta)
     gene            4203..4275
                     /locus_tag="VFJ72_03830"
     tRNA            4203..4275
                     /locus_tag="VFJ72_03830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4236..4238,aa:Thr,seq:ggt)
     gene            4313..4386
                     /locus_tag="VFJ72_03835"
     tRNA            4313..4386
                     /locus_tag="VFJ72_03835"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4347..4349,aa:Met,seq:cat)
     gene            4430..>4774
                     /locus_tag="VFJ72_03840"
     CDS             4430..>4774
                     /locus_tag="VFJ72_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7478630.1"
                     /translation="MSKGKFERNKPHLNVGTIGHVDHGKTTLTAAITKVLAKRVSDDP
                     ANKIVAFDQIDNAPEERQRGITIATSHQEYATANRHYAHVDCPGHADYVKNMITGAAQ
                     MDGAILVVSAADG"
BASE COUNT          871 a         1510 c         1643 g          750 t
ORIGIN      
        1 tccacctcgg cggcgcccgt accgcgctct tcaattacct cttcgcccgc catcacggag
       61 gtacgatact cctccgcgtc gaggacacgg acgccgcccg tagcacgcgg gagttcgaga
      121 agacgcagct agaagacatc gactggctcg gcctctcctt cgacgaaggg ccgtaccggc
      181 agagcgagcg cggcgactac tacgagagag ccgccgaacg gctcgccgga gcggggctga
      241 cctacgaagc cgaggatgag gttggacgcc gggcgctcta cctgcgtccg tccgagcgca
      301 gcggcagctt ccgggacgag ttgcgcggcg aggtccggtt ctcccgcatg gaggatttcg
      361 tgatccggaa gtccgacggc accccgtcct ataacttcgc ggccgtcgtg gacgacctgc
      421 agatgagcat aacccacgtc atccggggtg aggaacacct ctcgaacacc ggccggcagg
      481 ccgtcctcta ccgggcgctc ggcgaaaaag agccggagtt cgtacacctc ggcgtcatcc
      541 tcggcccgga cggcaagaaa ctcagcaagc ggcacggcgc ggcgagcgtc gcggactatc
      601 gggacgaggg ctatctgccg gaggcgctcg tgaactacct ggcgctcctc gggtggaacc
      661 acccggcggg ccgcgaggag ttcgacggcc tcgacgacct ggcgcgagag tgggacccgt
      721 cgcgtctcgg cgcgagttcc gccaccttcg acccggatcg cctgctgcac gtcaacggac
      781 ggcacatcag ggcactgccc gtcgcgcagt tgcgggagcg ggtggagccg tttttggaag
      841 agcctctgcc ggatggtcgg gggttgatct cggtagaagc catccgggac gagatgcgaa
      901 ccctctccga tgctccgcgg cttcttcgag agatcctcgg ccccgcggac ccggcggatt
      961 ttgccggcca gctacctgag tcgagcgata cggtcctcgc atacgtcctc gaagctctag
     1021 cgaagcgcag catcgaggac atcgaatcaa cccgcagctt cgtcgcggat ctcaggtcgt
     1081 gggcgaaggc gcgggggata aagacgcgag accttctcca tccgttgcgg ctggcgctca
     1141 cggggcgaga ccgggggccg gagatggcgt atcttttcgc ggtgctgggg ccggaggagg
     1201 cgcggagccg gatagaacgg gcgagagagg ctagactacg ggcatgagcg tgcgcgtgcg
     1261 ggacacattg agtggcagga tggtggaggt cggtcagagc gggaaggtcg gcatctacgt
     1321 ctgcggaccg acggtctaca accacatcca catcgggaac gcgcgggcgc cgctattctg
     1381 ggacgtggtg gtccggtatc tgaagagccg gggctactac gtgaccttcg tccagaacat
     1441 aacggacgtc gaggacaaga tcataggcaa ggccaatgag gaaggcgtct cgtggaaaga
     1501 ggtcgtggac cgctacaccg actccttcca cgagcggctg gagagcctcg gtgtcggact
     1561 gccggacgtg gagccgcggg ccacggagca catcccggag atgatccacc tgattgcaga
     1621 cctgatctca tccggccacg cctacgctcc gggtaacggc gacgtctact acgccgtcga
     1681 gagcttcccg ccctacggcg ccctgagcaa gcagcgtccc gccgagatgc gcgagaccga
     1741 gaagagcgcg accggctaca agaagagccc gctggacttc gcgctctgga aagcatcgaa
     1801 gcccggagag ccgtcgtggg agagcccgtg gggagcggga cgtcccggct ggcacatcga
     1861 gtgctccgcg atggtcgaga agcacctgcc cgacggcgcg gacatccacg gcggcggcac
     1921 ggacctgcgc ttcccgcacc acgagaacga gctggcgcag agcgccgccg cccacccgga
     1981 ccacactttc gtacgcgcgt gggcgcatca cggcatggtc aacttcgccg acaccggcaa
     2041 gatggcgaag tcgctcggca acatcctgga cgtggagcag gccgtcgagc gtcacggccg
     2101 caacgccctc aggatgtggc tgttgcagag ccactactcg caacccatag actactccga
     2161 ggagatcctg gaggagaagc gccgctccta tgagcggctg gtacgtctct accgccagct
     2221 ctccgcctcc acggacgact ctgaactctc cgagcgcctg gccgccgagc tacgcgagcg
     2281 tttcgacgct gcgatgcgtg acgacttcaa taccccggag gctgtcgccg ccctcttcga
     2341 ggtggcgaac cgggccgggc gcgaggtctc cgcccgtccc gaggcagcgc gggagttcaa
     2401 ggctctcgcc gcggctatcg ccgcggtcct gtccatcttc gggtttgatc tctccgagga
     2461 actctcggcg gagattgacg gtgtccgcat ccggtacgaa gaggacccag gggaagagtt
     2521 gctgcgccgc gtcgccggga gggagcgagc gcgcaaggag agagattggg ccaccgcaga
     2581 ccggctgcgc gacgagatcc aggctgccgg gtggtctgtc gaggacacct ccgaaggccc
     2641 gatcctgagc cgcccgtagg tgaagccgga ggttatctac ggtatccgtc ctgtggtcga
     2701 agcgctgcgg agcgtgcgcc gcagggtctt cgaggttctc gacgctgttg gggacgcgga
     2761 ggtggagaaa gccgcaagga cccgcggcgt cgccgtaaaa cgggcatccc gtcaacagat
     2821 cgaggacctc gcgcacggcg gcgtccatca gggcgtggcc gcgcgcgtcg agccgtattc
     2881 gtactcgggt ctcgacgaga tcctgaccgc ccccgagcct ctcgtactca tcctcgatgg
     2941 ggtgaccgac cctcacaacc tcggagcggt cctccgggtc gccgatggag cgggcgcgag
     3001 cggcgtcgtg atcccgaagg accgggccgt gggcgtgacg gcgagtgcgg taaaggccag
     3061 cgccggggcg agcgagcacg tgcgggtcgc gcgggagacg aacctgcggc gggccataga
     3121 tcggatgaag gaagccggtg tctgggtata tgccgccggg atggacggaa cgccctacac
     3181 taggctagat cttgccggtc cggtagcgtt cgtccttggc agcgaaggcc ggggtgttcg
     3241 gcgactcgtg cgcgagggct gcgatggggc ggtctccatc ccgatgctcg gggcggtcga
     3301 gtccttgaac gtctcggtgg ccacggcggt tctgatgtac gaggcgcgca ggcagagggg
     3361 atgacgtggc gtcgtacctg atcctcgacg cctacaacat catccgtgcc ctcgaccgct
     3421 acggcgagcg caacaccggc ggtttcgacg agtcccgtga gatgctgatc aacgacgcgc
     3481 tcaaggctgc gggctggacg ggacgggtca tcatcgtggt cttcgacgcg caccgcagtc
     3541 cggagcccag gcgagacgag tcgcgggccg gaggcgcggt gcgcgtgatc tacagtggcg
     3601 cggaagagtc ggccgacgat gtgatcgaac gtctcctcgg tacgctcgac ggcgcggcga
     3661 ccgtctacac cgccgacttt gccctccagc gcaccgccct cgcccgcggc tctacccgcg
     3721 ctacccccag cgagttccag gaactcttgg acgagctgcc gctactcatg agcagtccgt
     3781 caaagccccg gcgcgtcagg gttggggatt acctctcatc agataccctg cgggcactcg
     3841 aagaggtgcg ccgccgggcc gagagacgct aggatatact gcgatgctga gggctgatcg
     3901 ccggcaggcg ggcggcctgc aaatgcccgg gtagctcaga ggtagagcag ccgccttgta
     3961 agcggccggt cgggggttcg attcccttcc cgggctctgg ctccggcttc gtcccggcct
     4021 cgccggggga ctgttgaaaa gtcggtggcg tttgctacca taaccgggcg tttcggggcg
     4081 cggtccttcg ggctaccctg ggcgtggccg ggggtgtgcc cgagcggcca aagggagcag
     4141 actgtaaatc tgccggcttc gcctacggag gttcgaatcc tcccgccccc ataatcgaat
     4201 acgcctctgt agctcagtag gtagagcact tccatggtaa ggaaggggtc accggttcga
     4261 gtccggtcgg aggctctggg accggggtat actcggtcca cggcataggc gtggcggtgt
     4321 agctcagtcg gttagagcag cggaatcata atccgcgtgt cgcaggttcg agtcctgcca
     4381 ccgctacttt ctggtgtccg cgcggagttg agaaagagaa aggaaactga tgtccaaggg
     4441 caaatttgag aggaacaagc cgcacctgaa cgtggggacc atagggcacg tagaccacgg
     4501 caagacgacc ctcaccgcgg cgatcacgaa ggttctggca aagcgggtct ctgacgaccc
     4561 ggccaacaag atcgtcgctt tcgatcagat agacaacgct cccgaggagc gccagcgcgg
     4621 catcaccatc gcaacctccc accaggagta cgccacggcc aaccgtcact acgcccacgt
     4681 cgactgccca ggacacgccg actacgtaaa gaacatgatc acaggagcgg cccagatgga
     4741 cggggcgatc ctcgtggtat cggcagccga cggc
//
LOCUS       DASSMB010000056        10441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_266802, whole genome shotgun sequence.
ACCESSION   DASSMB010000056 DASSMB010000000
VERSION     DASSMB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10441
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266802"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="VFJ72_03845"
     CDS             complement(<1..539)
                     /locus_tag="VFJ72_03845"
                     /inference="COORDINATES: protein motif:HMM:NF021243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HET7478631.1"
                     /translation="MILRRLVEYAERLEAEGELTPPNYGSFRINWTIVLLLDGRLEGF
                     IPAPDKKGTEIIVPRHSVKRTSGIKADLLADEGEYVLGIPRDPGRSVQAAKQHAAFIE
                     LTRRCAEQTADPHVKAVLEFLEAWSHGEYRDRLPSELEPRDKMTFRVDGIIPAQDSDK
                     VREFWTEEQAPDAEGPRMM"
     gene            complement(536..1252)
                     /gene="cas5c"
                     /locus_tag="VFJ72_03850"
     CDS             complement(536..1252)
                     /gene="cas5c"
                     /locus_tag="VFJ72_03850"
                     /inference="COORDINATES: protein motif:HMM:NF021240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HET7478632.1"
                     /translation="METRDNGPPLQVKAWGEFACFTRPEFGVERVSYEVMTPSAARGV
                     LEAIFWKPEFRWRVREIEVLNPIRHFSILRNEMNSIQSDRSARSWAANGGGYFADDDR
                     AQRHTLCLRDVAYIIRADIELKPHAGEHPAKYRDQFRRRVKRGQCFNQPYLGTREFSA
                     FFGPPEGNERAIDVSDDLGLMLFDVDFVPDAKGAVSYAGHGADGREVVRGNAQPRFFQ
                     ARLENGVLRVPQEMYEGRRS"
     gene            complement(1264..3480)
                     /gene="cas3"
                     /locus_tag="VFJ72_03855"
     CDS             complement(1264..3480)
                     /gene="cas3"
                     /locus_tag="VFJ72_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HET7478633.1"
                     /translation="MQETRFFAHTPVEDGDWHDLLSHLKRTADLARGNGAKFGAGELA
                     RLAGLWHDIGKFNPEFQLYLRECEKAKRESTSAPAKGTPHAIYGAVLAYDSLQALAAI
                     IYGHHAGLPNGSKLRDAVRQSEVREKYESIVPVAREEIEDLDFRGDVRDLFVDPPRDE
                     LQMEMLQRMIFSALVDADFLDTETHFDPGMARLRGAEIRPEQLWDVFQRDRETFTASS
                     GERTTVNEVREEVYRFCVEAGERPQGVYRLGVPTGGGKTRSGLAFALRHAVEHSLDRV
                     IFAVPYTSIIEQTASVYREIFEELGESAVLEHHSAVRREEGESGERQDEIRTRSRLAA
                     QNWDAPLVVTTTVQLFESLFANRTSRCRKLHNLCRSVIVLDEVQTLPVGLLGPTVSAL
                     RELVRRYEVTVILCTATQPALDERSKYLEGFESIEDIVPAERAAEHFRTLRRVEYETP
                     QEKWYWDEVARQLLEASSEKQAMVVLNTRKDALALLEKLEGEPVLHLSTLLCGAHRRD
                     VLAEVRRRLEADEPCLLIATQVVEAGVDLDFPVVFRALGPLDRIVQAAGRCNREGKME
                     KPGRVVVFRPEEGRVPRGEYAAALGEAEIMLYRDGFDLHDPEIFREYFRLLYQDVPTD
                     TEGIQKLRREFDYPEVARLYRLISDASVPVIVRYPKEERRKPLENLLGRIRRTGLWSG
                     DHRRLQPYVVSMFEREFEKRREYTEEISEGVFVWNGNYDELRGIEDVSLDPADLVI"
     gene            complement(3545..7057)
                     /locus_tag="VFJ72_03860"
     CDS             complement(3545..7057)
                     /locus_tag="VFJ72_03860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET7478634.1"
                     /translation="MSVSGATFAHLHCHSEYSMLDGASRIKDLVSFAKDQNMPGLALT
                     DHGVMYGAVRFYKEAKDAGIKPVMGCEVYVTADRHDRSRAPYYHLTLLARTAEGYKNL
                     MKLSTCGFLEGFYYKPRVDMEMLRRHGRGIICLSGCLSAEVPTRILEGRPDEARKVLL
                     EYQEIFDAVYLEMQDHGISEQRRVNEGIIKLHREDGIPLVAANDSHYTTRSDAKMHDV
                     LLCIGTGKFYNDPNRMKFSGEEFYVKTVEEMARIFPDHPEALENTIKVIESVEDVGIE
                     LGKTRLPNFPKPEGYTADAYLREQCERGLAKRYGERAKSPEVRQRLEFELGTIGKMGF
                     ADYFLIVWDFVKYAKDQKIAVGPGRGSAAGSIVAYALEITDLDPLQYSLLFERFLNPD
                     RINMPDVDIDFSVGGRGEVMKYVTEKYGGHEHVAQIITFGTIGAKAAIRDSGRIYQYP
                     YSDTDKLAKFIPDKPVGTTLRDVLTPGEDGYVAGEKHPGAAREIIQYVQQNEAAKQVL
                     DTAFEIEGFARHAGTHAAGVVISEEKLTDIVPLQAVKKAGAKGEEEEHLSIMVQHPMS
                     DVEALGLLKVDFLGLRNLDVIEETLATIRQSGEEEPDIRTVPLDDEKTLGLFARGDTF
                     GVFQFESSGMQRMLQEVRPDRFDDLVALNALYRPGPMDYIPTFKRGKHDPESVKYLDE
                     RLKPILEPTYGVAAYQEQLMEISKTLGGFTPGEADTLRKAIGKKNAAMLATLKDKFME
                     GCAANDVSMDAADELWNWMEKAGGYSFNKSHSACYSFLAFQTAYLKAHYPEAYMAALM
                     SSVMNTKDRVPQYVAEARAMKIEVLPPDVNESGRRFTVVGGTIRFGLSAVKNVGENTV
                     EAIIAAREEGGPFTDIFDFCERVDSSTYNKRTVESLIKCGAFDNIGPSRAAMLAVHAK
                     AVERSTKGLKGASEDQFSMFDAAELAPPKPEFPPIEDDHRESLEWEKETLGLYVSDHP
                     LRPILHKLKKHTDTTVSELEGYRDGAVVWVGGLATSVRTNTTRKGDMMAMLQLDDTRG
                     LAEVIVFPRVYASCASCVREDAVLKVKGRVETKEGIPRIMALEIEELHLEPGPDPMYL
                     DAGAFVGLSRAAVQQAFEVLERHPGESPLSLVASNDGLEEKISTVEDSSDLHAELKQL
                     LGPRCLYYARPAAVPAEPEMEQVS"
     gene            complement(7332..7592)
                     /locus_tag="VFJ72_03865"
     CDS             complement(7332..7592)
                     /locus_tag="VFJ72_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478635.1"
                     /translation="MPQSTAHARHHSRNSGAVRRVLRALVLAVTLVFVLAALLISAAL
                     VVYTAWLHKIEEGAFALLFAVLSAQIAMNWILWRDDDEDPDE"
     gene            complement(7595..7897)
                     /locus_tag="VFJ72_03870"
     CDS             complement(7595..7897)
                     /locus_tag="VFJ72_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET7478636.1"
                     /translation="MPIYEYKCENGHVFDVMQKMSEDPLTECVECGAPVKKVLQPVGI
                     SFKGSGFYSTDYKNGSSKKAEPAAKTNGDSGSKSGSSETTSTKSESTASGKTSKKD"
     gene            complement(7979..8248)
                     /locus_tag="VFJ72_03875"
     CDS             complement(7979..8248)
                     /locus_tag="VFJ72_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478637.1"
                     /translation="MTEREERSFWNRLFSRDQHSEREERVLDYIQHRIGEGAHLADVV
                     QEEYVRRNASPGEVEEICARPELVQAAREHLQQDFNSEDLDPRRR"
     gene            complement(8329..8814)
                     /locus_tag="VFJ72_03880"
     CDS             complement(8329..8814)
                     /locus_tag="VFJ72_03880"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478638.1"
                     /translation="MPDLVVRPATEEDVPLILAFVRELAEYERLSHKVAATGEDLRRS
                     LFGERRFAEVLLAYLGEEPAGFALFFHNFSTFLGRPGIYLEDLFVRPEYRGAGVGRVL
                     LSHLARLAVERGCGRLEWWVLDWNEPAIGFYRKLGAAPMDDWTVYRVTGEALDRLAEG
                     A"
     gene            complement(8821..9792)
                     /locus_tag="VFJ72_03885"
     CDS             complement(8821..9792)
                     /locus_tag="VFJ72_03885"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET7478639.1"
                     /translation="MSDGYTVAVVGAGLVGDRLVSELRRREFPLKEFRILARSARRSV
                     LAGEEFEVAVAEPEAFEGVDFAFFAGTEGEKGAAVQLAQEAISRGAIVIDNGSDFRLD
                     PDVPLVVPEVNADALEGHNGLIANANCSTTQMVVALAPLAREFGLKKVVVSTYQAVSG
                     SGRGGVEALENGREDVYPKQISGNAIPLIGSIGDDGYSTEETKMREESRKILSLPDLE
                     VFATTVRIPVHTGHAESVYVETERETTLEEVLDALRDAPGIVFSGDANDFPTPLEAAG
                     EPGTYVGRVRVEGDVIQFWCVADNLLKGAATNAVQIAESLIGARVRA"
     gene            complement(9794..>10441)
                     /locus_tag="VFJ72_03890"
     CDS             complement(9794..>10441)
                     /locus_tag="VFJ72_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478640.1"
                     /translation="VGAGMPLISTIGGLIETGDEILEIQASPSGTLGFIMSAVEGGKS
                     FSEAVKEAVALHYAEPDPRDDLSGLDVARKAIILARMMGRKIEPEEVPYQSLVPEGLE
                     DVSLEEFMHRLPEADEAFAERLLAVEEHHMLRYLARIPREGQVEVGMVDEPVESPFGP
                     INGPENVFDLRTRRYSDVTLTIRGPGAGPERTASGVVWDILDIANKAVPDDGKDH"
BASE COUNT         1797 a         3587 c         3026 g         2031 t
ORIGIN      
        1 cacatcattc tgggcccctc tgcatcggga gcctgctcct ccgtccaaaa ttcgcggacc
       61 ttatcgctat cttgggcagg aataattcca tccaccctga aggtcatttt gtccctaggt
      121 tcaagttcac ttggcaatcg atctcgatac tcaccgtgtg accaagcctc aaggaactca
      181 agaaccgctt ttacgtgagg atcagccgtt tgttcggcac aacgacgagt aagttctatg
      241 aaagcggcat gctgcttcgc cgcttgcaca cttctaccag gatctctagg aatgcctaag
      301 acgtactcgc cctcgtctgc aagcagatca gctttgatgc ccgaggttcg cttgacgcta
      361 tgccgtggca ctatgatttc ggtacccttc ttgtccgggg caggaataaa tccttcaagc
      421 ctcccatcca gcaataggac tatggtccag ttgattctga aacttccgta attcggaggt
      481 gtaagctccc cttccgcctc caaccgctcg gcgtactcga cgagccggcg cagtatcatg
      541 agcgcctccc ctcatacatc tcctgtggga cccgcaggac gccattctcc aaccgggcct
      601 gaaagaaccg cggttgcgca ttgccccgga cgacctcacg accgtcggcg ccgtgtccgg
      661 cgtaggagac tgctcccttc gcgtccggca cgaagtccac gtcgaagagc atgaggccga
      721 ggtcgtcgct cacatctatc gcccgctcgt ttccctccgg cggcccgaag aacgcggaga
      781 actcgcgcgt gccgaggtac ggctggttga agcattggcc gcgcttgacc ctgcggcgga
      841 actggtcccg gtacttggct ggatgttcgc ccgcgtgcgg tttcaactcg atatccgccc
      901 ttatgatgta cgccacgtcg cgcaggcaga gggtgtggcg ctgggcgcga tcgtcgtcgg
      961 cgaagtatcc gccgccgttc gccgcccagg agcgggccga gcggtcggac tggatgctgt
     1021 tcatctcgtt gcggagtatc gagaagtgcc gaatggggtt gaggacttcg atctcgcgca
     1081 ccctccagcg aaactcgggc ttccagaaga tagcttccag cacgccccgc gcggcgctcg
     1141 gtgtcatcac ctcgtagctg acccgctcca ccccgaactc cggccgggta aagcacgcga
     1201 actctcccca cgctttaacc tgtagcggcg gtccgttgtc tctcgtctcc acaacctctc
     1261 ctttcagatg acgagatctg cggggtccag gctcacgtcc tcgatgcccc gtagctcatc
     1321 gtagttgcca ttccacacga agacgccctc ggagatctcc tccgtatact cccggcgctt
     1381 ctcgaactcg cgctcgaaca tcgagaccac gtaaggctgc aatcggcgat gatcgccgct
     1441 ccagaggccc gtgcgccgga tgcggccaag caggttctcc aagggtttcc tcctctcctc
     1501 cttcggatag cgcacgatca cgggcacgct ggcgtcggag attaaacggt aaagccgcgc
     1561 cacctccggg tagtcgaact cccgccgcag cttctggata ccttccgtat cggtaggcac
     1621 atcttgatag agcagacgga aatactcccg gaagatctcc ggatcgtgca ggtcgaagcc
     1681 gtctctgtac agcatgatct ccgcttctcc aagagcagcg gcatactcgc ctcgcgggac
     1741 tcttccctcc tccggcctga agacgaccac ccgcccgggc ttttccatct tcccctcccg
     1801 attgcagcgt ccggcggcct gcacgatcct gtccagaggc ccaagagccc ggaagacgac
     1861 aggaaaatcc aggtccaccc ccgcctccac cacctgcgtc gcaatgagca aacaaggctc
     1921 gtccgcttcc agccgccgcc ggacctccgc caacacgtct cgccgatgcg ccccgcaaag
     1981 cagtgtagac agatgtagga ctggctcccc ctccagcttc tccagaagcg ccagcgcgtc
     2041 cttgcgggtg ttgagcacga ccatcgcctg tttctcagac gaggcctcca gaagctgccg
     2101 cgcgacctca tcccaatacc atttttcttg cggcgtctcg tactcgacgc ggcgaagcgt
     2161 ccgaaagtgt tcggcggcac gctccgccgg gacgatatcc tcaatggact cgaagccctc
     2221 caggtacttg ctccgctcgt ccagcgcagg ctgggtggca gtgcagagga ttaccgtaac
     2281 ctcgtagcgg cgcaccaact cccgcagcgc gctcaccgtg ggaccgagaa gcccgaccgg
     2341 cagcgtctgg acttcgtcca gcacgatcac gctgcggcag aggttgtgca gcttgcgaca
     2401 ccggctcgtt cggtttgcga agaggctctc gaagagctgc acggtcgtcg taaccacgag
     2461 cggcgcgtcc cagttctgcg ccgcgagccg cgaacgggtc cggatctcgt cctgccgctc
     2521 cccgctttcc ccctcctccc gacgaaccgc cgaatggtgc tccagaaccg cgctctctcc
     2581 cagttcctca aatatttccc ggtaaacact cgccgtctgc tcgatgatcg aggtgtacgg
     2641 aaccgcaaag atcacccgat ccagactatg ctccacggcg tgacgcagcg cgaaagccag
     2701 tccgctcctg gtcttgccgc ccccggtagg cacgccgaga cggtagacac cctgcggtcg
     2761 ttcgccggcc tccacacaaa aacgatagac ttcttcccga acctcgttca cggtggttcg
     2821 ctcacccgag gaagccgtga acgtctcccg gtcccgctgg aagacatccc ataactgctc
     2881 cggtcggatc tccgcaccac gaagccgcgc catcccagga tcgaaatgcg tttcggtatc
     2941 caggaaatcc gcgtccacca gagccgagaa gatcattcgc tgcaacatct ccatctgcaa
     3001 ctcatcccga ggcgggtcca cgaaaagatc ccggacatcg ccccggaaat ccaggtcctc
     3061 gatctcctcc cgcgcaacgg gcacgatgct ttcgtacttt tcgcgaacct ccgactgtcg
     3121 caccgcgtcc cgcaacttcg atccgttcgg aagcccggca tgatgtccgt aaatgatcgc
     3181 cgccagtgcc tgtaacgaat cgtacgccag caccgcccca tagattgcgt gcggtgtgcc
     3241 tttcgccgga gcggaagtac tctcgcgctt cgccttctca cattctctca agtaaagctg
     3301 gaactccggg ttgaacttcc cgatatcgtg ccagaggccc gctagacgag ccagctcgcc
     3361 cgcaccaaac ttcgcgccgt tgcctcgtgc caggtcggca gttcgcttca ggtgcgagag
     3421 tagatcatgc cagtctccgt cctcgaccgg cgtatgagcg aagaaccttg tttcttgcat
     3481 acccacctcc cttgaaagat gagtatacat aagacctttg tgacgaccaa tactattttt
     3541 ggccctaaga gacctgctcc atctccggct ccgcgggcac ggccgccggg cgcgcgtagt
     3601 agaggcacct cgggcccagc aactgcttta gctcggcgtg caggtcggag gagtcttcga
     3661 cggtggagat cttctcctcc agaccgtcat tactggctac cagagacaac gggctctccc
     3721 ccggatgccg ctccagaacc tcgaacgcct gttgaaccgc cgcccgcgag agaccgacga
     3781 acgcgccggc gtccaggtac atcgggtccg ggccgggctc caggtgaagc tcctcgatct
     3841 ccagcgccat gatgcgcggg atgccctcct tcgtctccac ccggcccttg accttcagga
     3901 ccgcgtcctc ccgcacgcaa ctggcgcaac tggcgtagac cctggggaag actatgacct
     3961 cggccagccc ccgcgtgtcg tctagctgga gcatcgccat catgtcgccc ttgcgcgtgg
     4021 tgtttgtcct gacgctcgtg gcgagccccc cgacccacac gaccgcgccg tcgcggtacc
     4081 cctccagttc ggagacggta gtatccgtgt gcttcttgag tttgtggagg atcgggcgca
     4141 gcggatggtc cgagacgtag aggcccagcg tctccttctc ccactccagg ctctcgcggt
     4201 ggtcgtcctc gatgggcggg aactcgggct tcggcggcgc gagctccgcc gcgtcgaaca
     4261 tcgagaactg gtcctcgctc gcgcccttga ggcccttcgt ggaccgctcg acagccttcg
     4321 cgtggacggc cagcatcgcg gcccgcgacg gaccgatgtt gtcgaaagcg ccgcacttta
     4381 tgagcgactc gaccgtgcgc ttgttgtagg tactggagtc cacgcgctcg caaaagtcga
     4441 agatgtccgt gaacggaccg ccctcctcgc gggcggcgat gatcgcctcg accgtgttct
     4501 ccccgacgtt ctttaccgcg gagaggccga accggatcgt gccccccacc accgtgaacc
     4561 ggcgtccgct ctcgttcacg tccggcggca gcacttctat cttcatcgcc cgcgcctccg
     4621 cgacgtactg cggcacccgg tccttggtgt tcatgacgct gctcatcagc gccgccatgt
     4681 acgcctccgg gtagtgggcc ttcaggtacg ctgtctggaa ggcgaggaag gagtagcagg
     4741 cggagtggct cttgttgaac gagtagcccc ccgctttctc catccagttc caaagctcgt
     4801 cggcggcgtc catagagacg tcgttggccg cgcaaccctc catgaacttg tccttgagtg
     4861 tggcgagcat cgcggcgttc ttcttgccga tggccttgcg gagcgtatcg gcctcgccgg
     4921 gggtaaagcc gcccaatgtc ttggagatct ccatgagctg ctcctggtac gccgccaccc
     4981 catacgtggg ctccaggatg ggcttgagac gctcgtccag gtacttgacg ctctccgggt
     5041 cgtgcttgcc gcgtttgaag gtggggatgt agtccatcgg gccgggccgg tacagggcgt
     5101 tgagcgccac gaggtcgtcg aagcggtcgg gccggacttc ctggagcatc cgctgcatcc
     5161 cgctcgactc gaactggaag accccgaagg tgtcgcccct ggcgaagagc ccgagcgtct
     5221 tctcatcgtc cagcggcacc gtgcggatgt ccggctcctc ctcgccgctc tgccggatcg
     5281 tggcgagggt ctcctcgatg acgtcgaggt tccgcaggcc gaggaagtcg actttgagga
     5341 gacccaatgc ttctacgtcg ctcatcgggt gttggaccat aatggagagg tgctcctctt
     5401 cttcgccctt cgcgccagcc ttcttcaccg cctgcaacgg cacgatatcc gtgagcttct
     5461 cctccgagat cacgaccccg gcggcgtgcg tcccggcgtg ccgggcgaag ccctctatct
     5521 cgaaggcggt gtcgaggacc tgttttgcgg cctcgttctg ctggacgtac tggatgatct
     5581 cgcgcgccgc ccccggatgc ttctcccccg caacgtagcc atcctcgccc ggcgtgagca
     5641 cgtcgcgcag ggtcgtcccg acaggtttat ccgggatgaa cttggcgagc ttgtcggtgt
     5701 cggagtaggg gtactggtag atgcggccgg agtccctgat ggcggccttc gccccgatgg
     5761 tcccgaaggt gatgatctgg gctacgtgct cgtggccgcc gtacttctcc gttacgtact
     5821 tcatgacctc gccccgcccg ccgaccgaga agtctatgtc cacgtcgggc atgttgatcc
     5881 tgtccgggtt caggaaccgc tcgaagagca gagagtactg caagggatcg agatccgtta
     5941 tctccagcgc atacgcgact attgagccgg cggcggaacc gcgccccggc ccgaccgcta
     6001 tcttctggtc cttcgcgtac ttgacgaagt cccacacgat caggaagtag tccgcgaaac
     6061 ccatcttgcc gatggtcccc agctcgaact ccagcctctg gcgcacctcc gggctcttgg
     6121 cccgctcccc atagcgcttg gccagccccc gctcgcactg ctcccggagg tacgcatccg
     6181 ccgtgtagcc ctccggcttc gggaagttcg gcaggcgcgt cttgccaagc tctatcccca
     6241 cgtcctcgac gctctctatg accttgatgg tgttctcaag agcctcgggg tggtccggga
     6301 agatcctcgc catctcctcc acggtcttga cgtagaactc ctcgccgctg aacttcatcc
     6361 tgttcgggtc gttgtagaac ttgcccgtcc cgatgcacag gagcacgtcg tgcatcttcg
     6421 cgtcgctcct ggtagtgtag tgcgagtcgt tggcggcgac gagcggtatc ccgtcctccc
     6481 tgtgcagctt gatgattccc tcgttcacgc gccgctgctc ggagatgccg tggtcctgca
     6541 tctccaggta gacggcgtcg aagatctcct ggtactccag taggaccttc cgcgcctcgt
     6601 ccggccggcc ttccaagatg cgcgtcggga cctccgccga gagacacccg gagaggcata
     6661 tgatgcccct gccgtgccgc cgcagcatct ccatgtccac ccgcggcttg tagtagaagc
     6721 cttcgaggaa gccgcacgtc gagagcttca tgaggttctt atagccctcc gcggtgcgcg
     6781 ctagcagcgt gaggtgatag tagggagcgc ggctgcggtc gtggcggtcg gcggtgacgt
     6841 agacctcgca gcccatgacg ggctttatgc cggcgtcctt cgcctccttg tagaaccgga
     6901 cggcgccgta catgacaccg tggtcggtga gcgcgagtcc cggcatgttc tggtctttag
     6961 cgaaggagac gaggtccttg atgcgactcg ccccgtcgag catcgagtac tccgaatggc
     7021 agtgcagatg cgcgaaagta gctccgctga ccgacaaact ccctcctcca ctctttcggg
     7081 gcttcggcgg cgctcctcct cgtcgaagaa cgccgccggt cacctattct ccctatccat
     7141 cctggacgag agccactata cgcaacgcga gagcaccttg tctacctcac ctgaaccata
     7201 agttgagggc gtatttgcag gacttttcag gccatttata cgaacaaagg ctcgttatta
     7261 ccgaagatat tgggggacga gcagggtcga ggtctacatc tagacgctag agaaagcaca
     7321 gcgatttggg actattcgtc cgggtcttcg tcgtcgtcgc gccagaggat ccagttcatc
     7381 gcgatctggg cggagaggac ggcgaagagc agagcgaatg ctccctcttc gatcttgtgc
     7441 agccaggccg tatacaccac gagcgcggcg gagatgagga gagcggcgag gacgaagacc
     7501 agggttacgg cgagcacgag agcccgtaga acgcgccgga cagccccgct gttgcggctg
     7561 tggtgacggg cgtgggccgt gctctgtggc aaggtcagtc cttcttggag gtcttcccgg
     7621 aagcggtgct ctcggacttc gtggacgttg tctcggagga gccggacttg gagccggagt
     7681 ccccgttggt cttggcggcc ggctcggcct tcttggagct gccgttctta tagtcggtgg
     7741 agtagaaacc cgagcccttg aacgagattc cgaccggttg caagaccttc ttgaccggcg
     7801 cgccgcactc cacgcactcc gtaagagggt cttcggacat cttctgcatc acgtcgaaaa
     7861 cgtgcccgtt ctcacacttg tactcgtaga tcggcatcca gcctccagtt cacatactcg
     7921 caacccgtcg attatacaac ctctggaagt cggaagcctc tgcgtggggc ggctagggct
     7981 atctgcggcg cgggtccagg tcctcggagt tgaagtcctg ctggagatgt tcgcgagcgg
     8041 cctgaaccag ttccgggcgg gcgcagatct cctctacctc gcccggagag gcgttgcggc
     8101 gcacgtactc ctcctgcacc acgtccgcca ggtgcgcccc ctcgcctatg cggtgctgaa
     8161 tgtaatccag cacccgctcc tcgcgctccg aatgctgatc tcggctgaac agccggttcc
     8221 agaaagatct ctcttctctc tccgtcatac ctctcctctc ctcaccgctg gcgttccaac
     8281 tgagcaaagt ctagctagtc ggcgaccccg cgtacagagc ggcaaccctc aagcgccttc
     8341 tgccagccga tccaacgcct cccctgtaac ccggtagact gtccagtcgt ccatcggagc
     8401 ggctccgagc ttccggtaga aacctatcgc cggctcgttc cagtccagca cccaccactc
     8461 caggcgcccg caaccgcgct ccacggcgag ccgcgccagg tgcgacagca gaacgcgccc
     8521 gacccccgcg ccccgatact ccggcctcac gaacaggtcc tccaggtaga tgcccggccg
     8581 acccagaaac gtcgagaagt tgtggaagaa gagcgcgaac cctgcgggct cttccccgag
     8641 ataggccaaa aggacctcgg cgaagcgccg ctccccgaac agcgaccttc gcaagtcctc
     8701 cccggttgcg gctactttgt gggagagccg ctcgtactcg gcaagctccc ggacgaaggc
     8761 caggatcagg ggcacatcct cttcggttgc gggccggact acgagatccg gcacgcccgg
     8821 ttacgcccga actcgggcgc cgatcagaga ctccgcgatc tggacggcgt tcgtggcagc
     8881 gcccttgagc aggttatcgg cgacgcacca gaactggatc acgtctccct cgacccgcac
     8941 ccgcccgacg taggtccccg gctccccggc ggcctccagc ggcgtcggga agtcgttcgc
     9001 gtcgccggag aagacgatac ccggcgcgtc ccgcagcgcg tccagcactt cctccagcgt
     9061 cgtctcgcgc tccgtctcga cgtagacgct ctcggcgtgg ccggtgtgga cggggatgcg
     9121 gacggtggtg gcgaagactt ccaggtccgg gagggagaga atcttgcgcg actcctcccg
     9181 catcttggtc tcctccgtcg agtacccatc gtcgcctatg gacccgataa gcgggatggc
     9241 gttgccggag atctgcttcg gatacacgtc ctcccgcccg ttctccagcg cctcgacacc
     9301 gccccgaccc gacccggaca ccgcctggta agtcgagacg acgaccttct tcagcccgaa
     9361 ctcccgcgcc agcggggcca gcgcgacgac catctgggtc gtggagcagt tggcgttggc
     9421 gatgaggccg ttgtgacctt cgagcgcgtc ggcgttgacc tccgggacga cgagcgggac
     9481 atcggggtcc aggcggaagt cgctgccgtt gtctatgact atggcgccgc ggctgatggc
     9541 ctcctgcgcg agttgcaccg ccgcgccctt ctcgccctcg gtcccggcga agaaggcgaa
     9601 gtccacgccc tcgaacgcct ccggctccgc cacagccacc tcgaactcct cgccggccag
     9661 taccgaccgc cgggcgctcc gggcgagtat gcgaaactcc ttcagcggga actcccgccg
     9721 ccgcagctcg gagaccagcc ggtccccgac gagccccgcg cctactacag ctaccgtata
     9781 tccgtcgctc atgtcagtga tccttcccgt cgtccggtac agccttgttc gcgatatcga
     9841 ggatgtccca gacaaccccg ctcgcggtgc gctccggccc cgcgcccggc ccgcgtatgg
     9901 tgagggtaac atccgagtag cggcgcgtgc ggaggtcgaa gacgttctcg ggaccgttga
     9961 tcggcccgaa cgggctctcg accggctcgt ccaccatccc gacctcgacc tgaccctctc
    10021 tcgggatacg cgccaggtaa cgcagcatgt ggtgctcctc gacggccaga agccgctcgg
    10081 cgaaagcctc gtccgcctcg gggagccggt gcatgaactc ttccagcgac acgtcctcca
    10141 ggccctccgg gacgagagac tggtagggga cttcctccgg ttcgatcttc cgccccatca
    10201 tcctcgccag gataatcgcc ttccgcgcaa cgtccaggcc cgacaggtcg tcccgcgggt
    10261 ccggctccgc gtagtgcagc gccaccgcct ctttgacggc ctccgagaac gacttgccgc
    10321 cctcgacggc gctcatgatg aagcccagag tgccgctggg gctcgcctgg atctccagta
    10381 tctcgtcgcc cgtctcgatc aaccctccga tggtcgagat aaggggcatc cccgccccaa
    10441 c
//
LOCUS       DASSMB010000057        12174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1660326, whole genome shotgun sequence.
ACCESSION   DASSMB010000057 DASSMB010000000
VERSION     DASSMB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12174
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1660326"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VFJ72_03895"
     CDS             <1..1218
                     /locus_tag="VFJ72_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7478641.1"
                     /translation="HEKEESMIADVTRSLGTDYYLLDDLFTDEEREVRDKVRAFADRE
                     VIPVINEYWEKAQFPFELIPKIAELGIAGGAIEGYGCPGMSHTAAGLVAIEMSRGDGS
                     LNTFFGVHSGLAMGTIAICGSEEQKERWLPQMARLEKIGAWGLTEPDHGSDSVMLETS
                     ARREGDEWVINGRKRWIGNATFADLVIIWARDVEDGQVKGFVLEKDENGEHHPGYTTE
                     LIKGKMGKRAVWQPDIYLEDVRVPHENKLEGANSFKDTNKVLTATRGGVAWEAIGHAI
                     AAYEAAVQYAKERVQFGKPIASFQLIQNKLANMLAEITNMQLMTYRLSQLQEQGKMTG
                     PMASLAKMNNAKKAKQVCSDARDIMGGNGVLLEYHVARHLSDMEIVYTYEGTDTIQSL
                     IVGRDITGISAFV"
     gene            1387..2403
                     /locus_tag="VFJ72_03900"
     CDS             1387..2403
                     /locus_tag="VFJ72_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7478642.1"
                     /translation="MKAIRLHEIESPDKLVYEDVPEPEPGPGEVVVRLHNAALNRRDV
                     FVTRGMYPGAKPEALPVTLGSDGSGEVVARGDGADGPSEGTEVVINPALHWGDDPHVP
                     GKEYRILGLPDDGTFAQFVKAPADHVFPKPSHLSFEEAAAIPLAALTAYRALVTRGQL
                     QEGETVVIPGIGGGVATFLVQIAAALGARVFVTSGSDEKIEKAKDLGAEGGVNYESEN
                     WSKELRSMTGGVDLSVDSVGGEVFDTLITIAKPGGRVVSFGATAGPVPKLVLPKIFLK
                     HLTVLGTAMGTAEEFGAMLDLYQEHDLHPVINETFPLEEATAAMNHMEEGSGIGKIVL
                     DIPA"
     gene            2405..2809
                     /locus_tag="VFJ72_03905"
     CDS             2405..2809
                     /locus_tag="VFJ72_03905"
                     /inference="COORDINATES: protein motif:HMM:NF016178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET7478643.1"
                     /translation="MADYALEFGWPGDGGLGERLIGEVMSGEKTATCCPRSDYEEDEL
                     AEVISSEGEVLDVVDADGNVRCRVRMVAVYETTFGRPAPGLVRGEGFGSDRDAFRRDH
                     TQAWAEEMEEEGHPLTDDTILVVEEFELVEEE"
     gene            2925..5003
                     /locus_tag="VFJ72_03910"
     CDS             2925..5003
                     /locus_tag="VFJ72_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478644.1"
                     /translation="MLQHLILALLASAVVAVVPGYFWAASLCGGGLASRLTFSIGVSI
                     TLVPAIALIPARFFGFGVTLPAAIFSVLFVLASGLAVYLKSGAAKDPQEPIASPPAPF
                     GPLALILVLALLGLVLVVLFEIIPEEQAMVPLLILALFTGSAYLMEGGNATPVEPSAV
                     PPESRWRFLRGLVLPVVLALVLARGYLGPALHDWPYLRGTDQFNHGVMANEMLSSGAY
                     ESYLVYPPGFHTVIACISRLSGLRPLEIFPVLAPAFLILPCLAVYALANRLWGWGYAV
                     TAAAFAGLLLNSTYSNFAEARYPNFVSAEFLMVLAVAALAGLYAAPSIRTGLAFALLG
                     SSVVLVHPVGSLYMALLLALVSVLLLPYLLLYQRRLGITLLLSLALLGGISVLYAWDT
                     YDLGRLLNGLISGKGQGAGGAAVSQAIGTQEPLPLAHLTQSLSHPVLWLGAIGAVMLL
                     AGRSGRGLPAALSRVTLLLWGGILFVGSRTAMSGFPERFERDLGVPLALFAALAGVTA
                     LRSLRRRQSLAAWARPVTVVATAAVAVALALAVGVQGERNLRSAAEATPQALMTPDLA
                     AAGEWLRKHNDGGSIVAPAYIDRIPVRAVLAMGGYTELQTYRVVRIRLARSLPPTGAQ
                     PLWDGRWLLHHPDGERTHNIIEKYDIRYIVLHKPDQTIDWRAFVEKKDLYRVVYENEK
                     EIIVAPRDSS"
     gene            complement(4955..7090)
                     /locus_tag="VFJ72_03915"
     CDS             complement(4955..7090)
                     /locus_tag="VFJ72_03915"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HET7478645.1"
                     /translation="MIRFLRSPDLRVVSLALLAALLLVLLRGVGTAAPLLFLCAVALF
                     MAPGVMISRWFLREWSAGAALVPVAFAISAGMFGILGVPMLILNTSLDLYLILSGVIV
                     AAFLIAGIARAAVGASAGVVKSDVQAEDSAGLLWAPFAVLTAALAFVSWTKIPNLYDD
                     MWVYLSYVRAFLGDEPLAKYEPYFGQLTGIARLKINGWMLEQAALARVSGVDPVEMVI
                     KYLNPALVVVGLLAFYSLARTLFRSALAAVLASCLYALYFLVQLSDSVLPPGSEFAVR
                     LAEDKFAARFLFLPIALAAAVLFLRSRRGRYLAAFGLLCWAVVTVHPIGIAMIGVAMA
                     GFGLVYLAVNWRERGAWTKVAALGAAGASIGLLPLALVAATGERLADILRASDISSGD
                     PKVLANMVFVKPWRKKILEFSDGSYIMHPALLLDPTVLVALTAGLVFLLWRMRRSMTA
                     QLLLGTLVFVAIVDYVPQIATYVGDHFVLPWQLHRLAWSLSLAAMLTCCWMVWEAVRL
                     AQARMDGRYTGRVARLVPLLLVIVLMAVAAPWAVAGARDLAMGNRVQPGGVGLGFDPI
                     FPWMRDNIREPSVVLAPDAENTLIPAWSNKVNVVSLRGGLLFPVLERLKERTNGAIKA
                     PQGSIDVRTFYATPEIAPKIEVLRRYKVDYVLVFDGSPLEKQLRSIPGIEPVKGAPTD
                     RYSLYSVDLERLAGQDESLGATMISFSFS"
     gene            complement(7684..9018)
                     /gene="aceA"
                     /locus_tag="VFJ72_03920"
     CDS             complement(7684..9018)
                     /gene="aceA"
                     /locus_tag="VFJ72_03920"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HET7478646.1"
                     /translation="MDREQQIREIEQSWEGERWAGIERPYSAEDVAKLRGSVTIEHTL
                     ARLGAERLWELMHSEEYVNALGALTGNQAVQQVKAGLKAIYLSGWQVAADANLAGQMY
                     PDQSLYPANSVPHVVKRINQALQRADQIHHAEGKNSVNWFAPIVADAEAGFGGALNCF
                     ELMKAMIEAGAAGVHFEDQLSSEKKCGHMGGKVLLPTAQGIRNLISARLAADVMGVPT
                     IIVARTDADAANLITSDVDPADHRFLTGERTMEGFYRVNAGIDQAIARGLSYAPYADV
                     IWCETSTPDLDDARTFANAIHAEYPGKLLAYNCSPSFNWKKKLSDEDIATFQKQLGEM
                     GYKFQFITLAGFHALNYSMFDLARDYRERGMAAYSELQQKEFAAEKYGYTATKHQREV
                     GAGYFDEVAQVVAGGAASTTALTGSTEEAQFDTAEDPNTGLPGSTENAQFVK"
     gene            complement(9205..10803)
                     /gene="aceB"
                     /locus_tag="VFJ72_03925"
     CDS             complement(9205..10803)
                     /gene="aceB"
                     /locus_tag="VFJ72_03925"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HET7478647.1"
                     /translation="METKQRFGEGVEISAPIPDEFAEILTPEAVAFVAKLAREFTGRR
                     DELLQKRHERQERINAGEMPDFLPETKEIREGDWKIAPIPDDLQDRRVEITGPPDRKM
                     LINALNCGAPSYMTDFEDANCPTWQNMLDSQINLRDAIRRTITFDDPKSGKHYELNDE
                     VAVLIARPRGWHLDEKHMIVDGKPVPGGIFDFGLYFFHNAQPLLDIGSGPYFYLPKME
                     SHLEARLWNDIFVMAQDELGIPQGTIKATVLIETILATFEMHEILYELREHSSGLNCG
                     RWDYIFSYIKKFREHDMLLPDRNQITMTVPFMRAYSLLTIKTCHQRGAHAMGGMAAQI
                     PRSDDPEWTEFANNKVLEDKTREAKDGHDGTWVAHPGMVGLAKQAFDEHMPGPNQLDK
                     IPDVNPSAADLLEVPEGEITMDGFRNNVNVGVQYLAGWLAGRGAIPVFNLMEDAATAE
                     ISRAQVWQWIHHPKGILDDGTEVTVELFRKTLDEELVKIKEEVGQERWEKDDFERAAK
                     LFDKISTQDEFVEFLTLPGYEYFD"
     gene            complement(10915..11118)
                     /locus_tag="VFJ72_03930"
     CDS             complement(10915..11118)
                     /locus_tag="VFJ72_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478648.1"
                     /translation="MIPIQYRDPQTEEIIERRYEDGAPSIGQRVQIDSQEYQVLFRWR
                     CVPTSCIVYVRRVEQEMRATQAA"
     gene            11235..>12174
                     /locus_tag="VFJ72_03935"
     CDS             11235..>12174
                     /locus_tag="VFJ72_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7478649.1"
                     /translation="MLFRQVECFLAVARLGNVSRAAEEMFLTQPTLTARLKGLEDEVG
                     EQLFVRTSRGMRLTDAGREFVPYAERIVGGFEEGRRHLEELRGASGGRLMLGASPGVG
                     TYALPGLLERFVGAYPKVSVSVRTGHSEEILEMVLREEVQLGLTRAMSHPEVESLQLY
                     EDELVLVVDPDHRFTARGSASLADVAEETLILFDKDSSYTEQTHALFRNAAIREFRTM
                     EVDNIEAAKRMVEHRLGVAFLPRTAIVRSVSSGNLVLVNVEENPSLNRSIAALKRRDA
                     PASGPVAAFLEVAEKMGEQRSQTHLSPALTAEFENLQ"
BASE COUNT         2131 a         3914 c         3995 g         2134 t
ORIGIN      
        1 catgagaagg aggagtccat gatcgccgac gttacccggt ctctggggac cgactattac
       61 ttgctggacg acctgttcac ggacgaggag cgcgaggtcc gggacaaggt gcgggcgttc
      121 gcggatcggg aggtcatccc ggtcatcaac gagtactggg agaaggcgca gttcccgttc
      181 gagctaatac cgaagatcgc cgagcttggc attgcgggcg gggcaatcga gggctatgga
      241 tgtccgggga tgagccacac ggcggccggg ctcgtggcga tagagatgtc gcggggcgat
      301 gggagcctca acaccttctt cggggtccat tcggggcttg cgatggggac catcgcgatc
      361 tgcggcagtg aggagcagaa agagcgctgg ctcccgcaga tggcgcggct ggagaagatc
      421 ggggcctggg gcctcaccga accggaccac ggctccgatt cggtgatgct tgagacgagc
      481 gcccggcgcg agggcgacga gtgggtcata aacggccgga agcgttggat cggcaacgct
      541 accttcgcag acctggtgat catctgggcg cgggacgtgg aggacggtca ggtaaagggc
      601 ttcgtcctgg agaaggacga gaacggcgag catcaccccg gctacacgac ggagctgatc
      661 aagggcaaga tgggcaagcg ggccgtctgg cagccggaca tctacctgga ggacgtcagg
      721 gttccccacg agaacaagct cgaaggcgcc aactccttca aggacaccaa caaggtgctc
      781 acggcgacgc gcggcggcgt agcgtgggag gctatcgggc acgctatcgc ggcgtatgag
      841 gcggccgtgc agtacgccaa ggagcgggta cagttcggca agcccatagc cagcttccag
      901 ctcatccaga acaagctcgc caacatgctc gcggagatca ccaacatgca gctcatgacc
      961 taccgcctct cgcagctcca ggagcagggt aagatgaccg gcccgatggc atccctggcg
     1021 aagatgaaca acgccaagaa agccaagcag gtctgctccg acgcccgcga catcatgggt
     1081 gggaacggcg tgttgctgga gtaccatgtg gcgcggcacc tctccgacat ggagatcgtg
     1141 tacacctacg agggcacgga caccatccag tcgctcatcg tcggccgcga cataaccggc
     1201 atctccgcgt tcgtctagcg ggccagcttc cggctgatca agggtagggt cagggcgaca
     1261 ataagcgccg gtcggcgagc aaatcccggc cggcgctttc gccttgccgg tacggagccg
     1321 cgaaaaccat taagctgata gccgggtgtt gaaagctgac agcttctcga ctgaagggag
     1381 agacaaatga aagccatccg gctgcacgag atcgaaagcc ccgataagct cgtctacgag
     1441 gacgtaccgg agccggagcc cggtcccggc gaggtcgtcg tccggctgca caacgcggcc
     1501 ctgaaccgcc gcgacgtgtt cgtgacgcgc gggatgtacc ccggcgcgaa gccggaagcc
     1561 ctgcccgtca ctctcggctc ggacgggtcc ggcgaggtgg tggcgcgcgg cgacggcgcg
     1621 gacggcccgt cggagggtac cgaggtcgtc atcaacccgg ctctgcactg gggggatgac
     1681 ccgcatgtac cgggtaagga gtaccggata ctcgggctgc cggatgatgg aactttcgcg
     1741 cagttcgtga aggcgccggc ggaccacgtg tttccaaagc cctcgcacct ctcgttcgag
     1801 gaggccgcgg cgataccgct cgcggcactc acggcctacc gggcgctggt gacgcgcggg
     1861 cagttacagg aaggcgagac agtggtcatt cctggcatcg gaggtggggt ggcgactttc
     1921 ctggtccaga tcgcggctgc cctcggggcg agagtcttcg tcaccagcgg gagtgacgag
     1981 aagatcgaga aggcgaagga tctcggggcc gagggcggcg tcaattacga gtccgagaac
     2041 tggtccaagg agcttcggag catgacgggc ggcgtggatc tctcggtgga ttccgtgggt
     2101 ggcgaggtct tcgacacgct catcaccatc gcgaagcccg gcggccgggt cgtcagtttc
     2161 ggggcgacgg cgggtcccgt gccgaagctg gtgctgccca agatcttcct gaagcacctg
     2221 accgttctgg gcacggcgat ggggaccgct gaggaatttg gggcgatgct ggacctctac
     2281 caggagcacg acctgcaccc ggtaatcaac gagaccttcc cgctcgaaga agcgaccgcg
     2341 gccatgaacc acatggaaga gggctcgggc atcggcaaga tcgtgctcga catccccgcc
     2401 tgagatggcg gactacgcgc tggagtttgg atggccgggc gacggagggt tgggcgagcg
     2461 cctgatcggg gaggtcatga gcggcgagaa gaccgccacc tgctgccccc gctccgacta
     2521 cgaagaggac gagcttgcgg aggtgatctc tagcgagggc gaggtcctcg atgtcgtgga
     2581 cgctgatggg aacgtccggt gccgcgtgcg gatggtcgcc gtctatgaga ccactttcgg
     2641 acgtcccgcg ccgggcctcg tccggggcga gggcttcggg agcgaccgcg acgcattccg
     2701 gcgggaccac acgcaagcct gggccgaaga gatggaggag gagggccacc cgctcacgga
     2761 cgacaccatc ctggtggtcg aggagttcga gctggtagag gaggagtagg cggtgccggg
     2821 cgcgccgccc ccgccagtcg gaatgggtgc taccctggcg gggtgtatcc cctggacata
     2881 aaaaggctcg cgcactcacc cggcaagccg cagacaggcc gcggatgctt caacacctga
     2941 tcctggccct tctagcctcg gccgttgtag cggtcgtgcc gggatacttc tgggccgcgt
     3001 ctctctgtgg cgggggcctc gccagccgtc tcaccttcag catcggggtc tcgatcacgc
     3061 tcgtcccggc tatcgcgttg ataccggcac gcttcttcgg cttcggagta acgttgccag
     3121 cggccatctt ctccgtgctg ttcgtcctgg cgtccggcct cgccgtctac ctgaagtccg
     3181 gcgcggcgaa ggatccgcaa gagcccatcg catcgcctcc cgcgccgttc ggaccgctcg
     3241 ccctgatcct ggttctggcc ctgctcgggc tcgttctggt ggtgctcttc gagattatcc
     3301 ccgaggaaca agcgatggtt ccgctactga tattagctct gttcaccggg tccgcgtatc
     3361 tgatggaggg cggaaacgcc acgccagtgg agccgtcggc agttccgccg gagagccggt
     3421 ggaggttcct gcgtggcctg gtgttgccgg ttgtactggc tctggttctc gcgcgcggat
     3481 acctcggtcc ggcgcttcac gactggcctt atctgcgggg gacggaccag ttcaatcacg
     3541 gggtaatggc taatgagatg ctctcgtcgg gagcttatga gtcgtacctg gtctatccgc
     3601 cgggcttcca cacggtgatc gcgtgtatat cccgactgag cgggctgcgt ccgctggaga
     3661 tcttcccggt cctcgccccc gctttcctga tactcccgtg cctcgcggtc tacgcgctcg
     3721 ccaaccgtct ctggggatgg ggctatgcgg tgacggccgc cgccttcgcc gggctcctcc
     3781 tgaacagcac gtacagcaac ttcgccgagg cccgatatcc gaacttcgtc tcggccgagt
     3841 tcctgatggt cctagccgtc gccgccctgg cggggctcta cgccgcgcct tccatccgca
     3901 cggggctggc gttcgcctta ctcggctcgt cggtcgtcct ggtccatccg gtcgggagcc
     3961 tctacatggc cctgctgctc gcgctggtga gcgtgctgct cctgccgtac ctgctgctgt
     4021 accagcggag gctcggcatt acattgcttc tctcgctggc gctgctaggc gggatctcgg
     4081 tcctctacgc ctgggatacc tacgacctgg gccggctgct caacggtctg atctcgggga
     4141 aaggacaggg cgcgggcggc gcggcggtct cgcaggccat cggcacccag gagccgctgc
     4201 cgctcgcgca cctgacacag tccttgtcac accccgtgct gtggctggga gcgatcggag
     4261 ctgtaatgct gctggccgga cggagcggcc gcggcctgcc cgcggcgctg tcgagagtaa
     4321 ccctgctcct gtggggcggc atcctgttcg tcgggagccg cacggcgatg agcggcttcc
     4381 cggagcgctt cgaacgcgac ctcggcgtcc cgctcgctct cttcgccgct ctcgccggcg
     4441 tgacagccct gcggtcgctg aggaggagac agtcgctggc ggcatgggcc agaccggtga
     4501 ccgtcgtggc gaccgccgcg gtcgcggtag cgctggcgct ggcggtggga gtgcagggag
     4561 aacggaacct gaggtctgcg gcggaggcaa ccccccaggc cctgatgact ccggacctcg
     4621 cggcggcggg agagtggctg cggaagcaca acgacggcgg gagcatcgtc gccccggcgt
     4681 acatagaccg catcccggtg cgggccgtgc tcgcgatggg cggttacacg gagctacaga
     4741 cgtacagggt ggtgaggata cggctcgccc gctcgctccc cccgaccggc gcgcagccgc
     4801 tctgggacgg gcgctggctc ctgcaccacc cggacggcga acgcacacac aacatcatcg
     4861 agaagtacga catccggtac atcgtgctgc acaagcccga ccagacaata gattggcgcg
     4921 cgttcgtgga gaagaaggac ctgtaccggg tagtctacga gaacgagaaa gagatcatcg
     4981 tcgcccccag ggactcatcc tgaccggcca gacgttccag gtccacggag tagaggctgt
     5041 agcggtctgt gggggctccc ttcaccggct cgatccccgg aatgctgcgt agctgcttct
     5101 cgagcggcga gccgtcgaag accagcacgt agtctacctt gtagcgacgc aggacctcga
     5161 tcttcggcgc tatctcgggc gtagcgtaga aggtccgcac gtctatgctt ccctgtgggg
     5221 ccttgatggc tccgttcgtg cgctccttga gcctttccag gaccgggaag agcagaccgc
     5281 cgcgcaggct caccacgttc accttattgg accaggcagg gatcagggta ttctccgcgt
     5341 ccggcgccag cacgacgctc ggctctctga tattgtcccg catccagggg aatatggggt
     5401 cgaagcccag ccccacgccg cccggctgca cccggttccc catcgccagg tctctcgccc
     5461 ccgccacggc ccacggcgcc gccaccgcca tcagaactat gacgagcagc agcgggacta
     5521 accgggcaac gcgcccggtg tagcgtccat ccattcgggc ctgcgcgagg cgcaccgcct
     5581 cccacaccat ccagcagcag gtcaacatcg cggcgagcga gagcgaccac gctagacggt
     5641 gtagctgcca gggcagaacg aagtggtctc cgacataggt ggcgatctgg ggcacatagt
     5701 ccactatcgc cacgaagacg agcgtcccga gcagcaactg ggccgtcatg ctgcgcctca
     5761 ttcgccagag caggaatacc agtccggcgg tcagagccac cagcaccgtc gggtcgagca
     5821 ggagggccgg gtgcatgatg tacgagccgt cgctgaactc caggatcttc ttcctccagg
     5881 gcttcacgaa gaccatgttg gcgaggacct tcgggtcccc ggagcttata tccgaggcgc
     5941 gcaggatgtc cgcgagccgc tcaccagtcg ccgcgacgag cgccagaggc agcagcccta
     6001 tgctcgcgcc cgcggcgccc agggccgcaa cctttgtcca cgccccgcgc tcccgccagt
     6061 tcaccgccag atagacaaga ccgaacccgg ccatcgcgac gcctatcatt gcgatgccga
     6121 tgggatgcac ggtaacgacc gcccagcaca gtaacccgaa cgcggcgaga tacctcccgc
     6181 ggcggctccg caggaagagc accgccgcag ccagagcgat gggcaggaag aggaaccggg
     6241 ccgcgaactt gtcctccgcc agccgcacgg cgaactcgct ccccggcggc agcaccgagt
     6301 cgctcaactg gaccaggaaa tacagcgcgt acaggcagct cgccaggacc gccgccaacg
     6361 cgctcctgaa cagggtgcgc gccagcgagt agaaagccag caggcccacg accacgagcg
     6421 cggggtttag gtacttgatc accatctcca ccgggtccac gccggagacg cgggccagcg
     6481 ccgcctgctc cagcatccag ccgttgatct tcaaccgcgc gatccccgtc aactgtccga
     6541 agtacggctc gtacttcgcc agcggctcgt cccccaggaa cgcccgcaca taggacagat
     6601 agacccacat atcgtcgtag aggttcggga tcttcgtcca ggacacgaac gcgagagcgg
     6661 cggtaagtac cgcgaacggc gcccagagaa gcccggccga gtcctccgcc tggacatcgc
     6721 tcttcacgac gccagccgaa gccccgacgg ccgcgcgagc gatccccgct atcaggaaag
     6781 cggcgacgat gacgccggag aggatcaggt acaggtcgag gctcgtgttc agaatcagca
     6841 tcgggacgcc gaggatgccg aacatcccgg cgctgatggc gaacgccacc ggaaccagcg
     6901 ccgcccccgc actccactcc cgcaagaacc agcgcgagat catcacaccc ggcgccataa
     6961 acagcgccac cgcgcagaga aagagtaacg gagccgccgt gccgacaccc cgcaagagca
     7021 ccaacaagag agcggccagg agagcaagcg acacaacccg gaggtcgggc gagcggagaa
     7081 agcggatcac accctctccc ctacgcgcac cggacacccc cggctccgct ccgcgatgcc
     7141 atttcataag gctttaacac tagcgtcggt ttcggcgcac gccgtaccgg cacaccggtc
     7201 ctgcgcgaga tctccttcac gaacatcctt atgttggata cgtcccgaca ccggcattat
     7261 caacacaggc ggcacggaag ttcaatgcgc atcggtgcgt tccaccgcta aagattccac
     7321 agctcccggt aaacgccgag caccaaccgg cggcattcct ccggtagatc tccgaagcag
     7381 atctcgcgct tcatcaaccc gtacggcggt tctcctcgac gcagcacacc gtcggactcc
     7441 tccggttccg tccgcatagt gaggtagcca tactgacccg gctccttgag cgagtagagt
     7501 cccgtgaaca ctgcggtata gcgttcggcg gtcacgccgc cgctgtcgta gagtcagacg
     7561 tacagcggga tgccatccgg taacagccgt tcttgcctcg tccgtaagct catgacgaga
     7621 ttatgcacga agagagggac cggcgaaccg atccctctgc tgaactgcca gccggctgaa
     7681 aagctacttc acgaactgcg cgttctccgt ggagccgggc aggccggtgt tcgggtcctc
     7741 ggccgtgtcg aactgagcct cctcggtgga gccggtgagg gccgtggtgg aggcggcgcc
     7801 tcccgcgacg acctgggcga cctcgtcgaa gtagcccgcg ccgacctcgc gctggtgctt
     7861 ggtggcggtg taaccgtact tctcggcggc gaactccttc tgctgaagct cggagtaggc
     7921 ggccataccg cgctcgcggt agtcccgcgc caaatcgaac atgctgtagt tgagggcgtg
     7981 gaagccggcg agcgtgatga actggaactt gtaacccatc tctccgagct gcttctggaa
     8041 ggtggcgatg tcctcgtcgg agagcttctt cttccagttg aacgacggcg agcagttgta
     8101 ggcgagcagc ttgccgggat actcggcgtg aattgcattg gcgaaggtgc gggcgtcgtc
     8161 gaggtccggc gtggaggtct cgcaccagat cacgtcggcg tacggcgcgt aggagaggcc
     8221 gcgggctatg gcctgatcta tccccgcgtt cacccggtag aagccctcca tcgtccgctc
     8281 cccggtcagg aacctgtggt ccgccgggtc aacatcgctg gtgatgaggt tcgcagcgtc
     8341 ggcgtcggtc ctcgcgacga tgatcgtcgg gacgcccatc acgtccgccg cgagccgcgc
     8401 ggagatgagg ttcctgatgc cctgggccgt cggcaagagc accttaccgc ccatgtgccc
     8461 gcacttcttc tccgacgaaa gttggtcctc gaagtggacg cccgccgctc cggcctcgat
     8521 cattgccttc atcaactcga agcagttgag cgcgccgccg aacccggcct cggcgtcggc
     8581 gactatgggc gcgaaccagt taacggagtt cttgccctcg gcgtggtgga tctggtccgc
     8641 ccgctgcaaa gcctggttga tcctcttgac cacgtgcggc acgctgttgg ccggatacaa
     8701 gctctggtcc gggtacatct gccccgccag gttagcgtcc gccgcgacct gccagccgct
     8761 gaggtatata gccttcaaac ccgccttgac ctgctgcacc gcctggttgc cggtgagggc
     8821 gccgagagca ttcacgtatt cttccgaatg catcaactcc cagagccgct ccgccccgag
     8881 ccgggcgagc gtgtgctcta tcgtcaccga gccccggagc ttcgccacgt cctccgccga
     8941 gtacggacgc tctatccccg cccagcgctc gccctcccag ctctgctcta tctccctgat
     9001 ctgctgctct ctgtccacta catctccttc tgtaaaaacc atctacaaaa aacttgcttc
     9061 acgcacatag gtgctcacat atgtgcatag cgtgcgcgca acatacgtgg cccacgctcc
     9121 ggggcaggag gacggggtcc ggcggccgga ctgctccgcc gccggtcctc gttgttatgt
     9181 cggtgaggta ggggatctcc gctcctagtc gaagtactcg tagccgggga gggtcaggaa
     9241 ctcgacgaac tcgtcctggg tagagatctt gtcgaagagc ttggcggctc tttcgaagtc
     9301 gtccttctcc cagcgctcct ggccgacctc ttccttaatc ttcaccagct cctcgtcgag
     9361 agtcttgcgg aaaagttcga ccgtgacctc ggtgccgtcg tcgaggatgc ccttggggtg
     9421 gtggatccac tgccacacct gcgcgcggct gatctccgcc gtcgccgcgt cctccatgag
     9481 gttgaagacg gggatggctc cgcgtccggc cagccagccg gccaggtact gcacgccgac
     9541 attgacattg ttgcggaagc cgtccatcgt gatctcgccc tccggcacct ccagcaggtc
     9601 cgccgccgaa gggttcacgt cggggatctt gtcgagctgg ttcggtcccg gcatgtgttc
     9661 gtcgaatgcc tgcttcgcaa gcccgaccat gcccggatgg gccacccagg tcccgtcgtg
     9721 gccgtccttc gcctcgcgcg tcttgtcctc cagcaccttg ttgttggcga actcggtcca
     9781 ctccgggtcg tcggagcgcg ggatctgggc cgccattccc cccatcgcgt gcgcgccgcg
     9841 ctggtgacag gtcttgatcg tcaggagcga gtacgcccgc atgaacggga cggtcatcgt
     9901 gatctggttg cggtccggga ggagcatgtc gtgctcgcgg aacttcttga tgtaggagaa
     9961 gatgtagtcc caccggccgc agttcaggcc cgaggagtgc tcgcgcagct cgtacaggat
    10021 ctcgtgcatc tcgaacgtcg ccaggatggt ctctatgagg accgtcgcct tgatggtccc
    10081 ctgcggtatc ccaagctcat cctgggccat gacgaagatg tcgttccaga gccgcgcctc
    10141 caggtggctc tccatcttcg gcaggtagaa gtacgggccg ctgccgatgt ccagaagcgg
    10201 ctgcgcgttg tggaagaagt acaagccgaa gtcgaagatg ccgccgggga ccggcttgcc
    10261 gtccacgatc atgtgcttct cgtccaggtg ccagccgcgg ggacgggcga tgaggacggc
    10321 gacctcgtcg ttgagctcgt agtgcttgcc gctcttcggg tcgtcgaacg tgatggtgcg
    10381 ccggatggcg tcccgcaggt tgatctggga atcgagcatg ttctgccagg tggggcagtt
    10441 ggcgtcctcg aagtccgtca tatacgacgg ggcgccgcag ttgagagcgt tgatgagcat
    10501 cttccggtcc ggcggaccgg tgatctccac gcgccggtcc tgaaggtcgt cggggatcgg
    10561 cgcgatcttc cagtcgccct cccggatctc cttcgtctcc ggcaggaagt ccggcatctc
    10621 gccggcgttg atcctctcct gccgctcgtg gcgcttctgc aagagttcat ccctgcgccc
    10681 cgtaaactcc cgcgccagct tcgccacgaa agcgaccgcc tcgggcgtca ggatctcggc
    10741 gaactcatcg gggatggggg ccgagatctc gaccccctct ccaaaccgct gcttggtctc
    10801 catagtttgt gcgggctcct ctctgcctct cctacgactt ccgctttagc gaacgctaga
    10861 gcaaagctac tctagcaagc ggccgcaggc ggcgcttgga gcccgagaac ctctctaggc
    10921 ggcctgtgtg gcccgcatct cctgctcgac ccgccgcaca tacacgatgc aactcgtcgg
    10981 cacgcaccgc cacctgaaga gcacctggta ctcctgagaa tctatctgca cccgctgccc
    11041 gatggacgga gcgccatcct cgtagcgccg ctcgatgatc tcctccgtct gcgggtccct
    11101 gtactgtatc ggaatcatat tgctctcctc tcaaccgctc ctgactgtgc aagcaagata
    11161 acaataccct tgatattagt ccaattgttt gcttgaatca ttgttatagg cgaaatttat
    11221 atcaggaggc agtggtgctg ttcaggcagg tggagtgttt tctggcggta gcgcggctgg
    11281 ggaacgtgag ccgggcggcg gaggagatgt tcctgacgca gccgaccctc acggcgcggc
    11341 tgaagggttt ggaggatgag gttggggagc agctcttcgt ccggacgagc cgtgggatgc
    11401 ggctgacgga tgcgggacgg gagttcgtgc cgtatgcgga gcggatcgtg ggcggtttcg
    11461 aggaggggcg gcggcacctg gaggagttgc ggggcgcatc ggggggacgg ctgatgctgg
    11521 gagcatcgcc gggcgtgggg acgtatgcgc tgccggggct tctggagcgg ttcgttgggg
    11581 cgtatccgaa ggtatcggtc tccgtaagga cggggcactc cgaggagatc ctggagatgg
    11641 tgctccggga ggaggtgcag ctcgggctga cgcgggcaat gagccacccg gaggtggaga
    11701 gtttgcagct ctacgaggac gagctggtgt tggtggtgga cccggaccac aggtttacgg
    11761 cgcgggggtc ggcgagcctc gcggacgtgg cggaggagac gctcatcctc ttcgacaaag
    11821 actcaagcta cacggagcag acgcacgcgc tgttccggaa cgcggccatc cgggagttcc
    11881 gcacgatgga ggtggacaac atcgaggcgg cgaagaggat ggtcgagcac cgcctggggg
    11941 tggcgttcct gccgcgcacg gcgatagtgc gcagcgtctc gtccgggaac cttgtgctgg
    12001 tcaacgtcga ggagaacccc tcgctgaacc gctccatcgc ggccctaaag cgccgggacg
    12061 cgcccgcgtc cggtcccgtg gccgcgttcc tggaggtcgc ggagaagatg ggcgagcagc
    12121 gcagccagac ccacctctcc cccgccctga ccgccgagtt cgagaacctg cagc
//
LOCUS       DASSMB010000058        34779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1028025, whole genome shotgun sequence.
ACCESSION   DASSMB010000058 DASSMB010000000
VERSION     DASSMB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 34779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34779
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028025"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFJ72_03940"
     CDS             <1..251
                     /locus_tag="VFJ72_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478650.1"
                     /translation="SEGPVLGSAMLAAVGAGIYPDIQAAADNMVHTERTIEPNLEAHE
                     AYKFYLDRYIETYPAMKELMHKTVRHEASGHETAAKEA"
     gene            368..1429
                     /locus_tag="VFJ72_03945"
     CDS             368..1429
                     /locus_tag="VFJ72_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478651.1"
                     /translation="MTTVTVLGSGIMATALAWPLSDNGHEVRLVGTHLDREIVDSIKE
                     SGVHPNLDLKVPDGVRAYQLEEAEEAFEGAEIVMSGVNSFGVRWASEHLASLLKPGVM
                     VIAVTKGMDADEDGNLRILPEVLAEQVPQDVRDQVSWSAIVGPAIAGEVAVRHDTCVV
                     FAGEDADALEKLAATFRTEYYHVWTSTDFVGCEVCAATKNCYAFGGGFMEGLLDREGK
                     TDARYRRYNYGAALFGQATRELGQWMELLGGDPETPHGLPGVGDMFVTTMGGRNVKAG
                     RFVGAGVPFSEVRDEKMKGVTLEGVAAIEVIGGALPKLTERGVVAEEDFPLLRHLYEV
                     VALDRPVDMPWSTFFGGEA"
     gene            1454..3223
                     /locus_tag="VFJ72_03950"
     CDS             1454..3223
                     /locus_tag="VFJ72_03950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET7478652.1"
                     /translation="MNRESLLQHVGERPFDLIVIGAGINGAGIARDAAMRGLRVLMLD
                     KGDIASGTTQWATRLIHGGLRYLEHYEVPLVRESLADREILLHIAPHLVKPLKFTVPI
                     YDRSSRGPGMIRLGMIGYDTLSYDSSMPRHKMLSREEALREYPGLNPEGLKAAATYYD
                     GQVEYAERIAVENAVSAVQNGAVVITYAEVKRLLFEDGGSRVAGVEFEDKLEGGVYSV
                     RAPVTVNVAGPWVDRVLGGSGAVSVETPGNGANGDSGGMIGGTKGSHIIVDPFPGAPK
                     NAIYVEARKDGRPYFIVPWNERYLIGTTDLRYRDDLDYVSASEEEIQYLIDETNYVIP
                     DAHLTRDSVLFTYSGVRPLPSAPAGSEGSITRAHVVYDHAKGKSVAGGRIKVSGGGGP
                     RAEGLLSIVGGKLTTYRNLGRQATNVVYKKLGAKPPKSATDRIPLPGGRTPDFAAFTE
                     EFRSRSGLPDVLARRLIRLYGARAYDVLAEAGDDSSLREPLVSPPAVETGLMGCEVLY
                     AFRQEMAQKLSDVLLRRTMVAYGPQVALDVDEAAAAVAVKHLGWSEERAKKEVAEYRE
                     WIQRYTPKALREREGAGAPGSAE"
     gene            3279..4034
                     /locus_tag="VFJ72_03955"
     CDS             3279..4034
                     /locus_tag="VFJ72_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7478653.1"
                     /translation="MISAVLFDLDGTLLETEELKGLSYARSVTELRPDVAEEDVMSAY
                     ADDLVGHSRQEVATDLMRRFQLEEAARGRMDEFGEEEPWRVLVKIRLGVYEALLEDSD
                     LLLEKRYPHNIEMLRELRREGYPTACATMSHRYQVERVLSVLDLEDAFDVVATADDIK
                     RGKPDPEIDLLVAEKMGVPPEEFLVIEDSPAGVRAALAAGMMVVAVPTKLTVRGVRAA
                     GILEPRWVVEDPEMLPRVVRQRIEEAGGRSEGG"
     gene            4185..4991
                     /locus_tag="VFJ72_03960"
     CDS             4185..4991
                     /locus_tag="VFJ72_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7478654.1"
                     /translation="MRIVQLSDIHTGTPLFRPDFLQAAIEETNALEPDLVAVAGDLTT
                     EGYRWEFEEAKGYLDRIECENVVVIMGNHDAKNVGYRHFEDFFGPRMRAMTVPIPDGE
                     AKVVALDSSKPDLAEGEIGREHYPWIDSEFRGWDRGPKILVVHHHILAVPGTGRDRNN
                     LRDSGDIMAVLRELEVDMVLAGHRHVPYVWSISGVRIIHSGTVSSMRVRGIIPPSYNV
                     IDFGPDKVEVTLRHPGGEQEPLARFSRRAVTTSEIYPELDRFVRYDEVPV"
     gene            5045..5866
                     /gene="otsB"
                     /locus_tag="VFJ72_03965"
     CDS             5045..5866
                     /gene="otsB"
                     /locus_tag="VFJ72_03965"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET7478655.1"
                     /translation="MPREIGDLPHAIENEGELEQRLAGKRLAVFLDYDGVLTPIVDRP
                     EDAIISEGMREVISNLAERCPVCVVSGRDRQVVQQLMGLENLIVAGSHGFDIWSPGEG
                     TIQHDAGAGFEDLLEDVRDYLQSEAAPIEGSSIETKKASVAIHYRLVAEEERPKIKEI
                     VDSLLEERPDELKVTPGKMVYEVQPKIDWHKGKAVLYLLEALDLDGDDVMTMYLGDDV
                     TDENAFEALNETSPKGIGIFVGEADHPEVVGRTTSADYILYTVPEVETFLDGLAR"
     gene            6076..8472
                     /locus_tag="VFJ72_03970"
     CDS             6076..8472
                     /locus_tag="VFJ72_03970"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HET7478656.1"
                     /translation="MTNWTLSYDYYDPEEEGLREAMSSTGNGYFCTRGTAHWADADSV
                     NYPGTYVHGGFNRETTIMAGRPVLNEDLVNLPNWLVLKLRIEGDGEPIDFGNVEILSY
                     QHEYDIRNATLTRNLRFRDREGRETSLESRRFVSMADMHQAAIEWKITPENWSGKVEI
                     ITAVDGRVTNGGVARYKELEGRHLDPVSPRTFGPEIIALLVRTRQSRIYVAEAARTRV
                     YGENGMISLDRDLHQMQDYIHQVLGFDVEEKSTVRVEKMVSFYTSRDHAINEPLSNAG
                     RAVMRYGTFEEAFEQHARVWDELWDACDMKIPGDDRVQLLVRLHISHILQVCSPHTAD
                     LDAGAPARGLNGEAYRGHIFWDELYIYPFLNFRLPQITRELLVYRYRRLGEARAAAKE
                     VGYRGAMYPWQSGSDGSEETQKVHLNPNSGNWDPDLSHRQRHVGAAIFYNIWHYYQVT
                     GDMEFLIDHGAEMMLEIARFWSSIAHYNDERDRYEIHGVMGPDEFHEKYPGSEEEGLR
                     NNAYTNVMVAWICESAQEVLKMLPEKRRDSLRSKIGLSDDEVELWGEMSHKMFVPFHD
                     DGIISQFEGYENLEELDWDAYREKYENIQRLDRILKAEGDDPDRYKLAKQADTLMLFF
                     LFPEDQLKRLFERLGYDYKPDTARKNIEYYAQRTSHGSTLSFIVHAAIMADLDPESSW
                     KRYMVALESDIGDVQGGTTREAIHMGVMAGTLDLLQRGYMGTEIRDGVVHFAPKLIKK
                     LEGLSFPMRFRETPMEITLESGKLAVAAHSDGLNQCIKVGVGDTVQEIRAGESHTFSI
                     "
     gene            complement(8895..9932)
                     /gene="nrfD"
                     /locus_tag="VFJ72_03975"
     CDS             complement(8895..9932)
                     /gene="nrfD"
                     /locus_tag="VFJ72_03975"
                     /inference="COORDINATES: protein motif:HMM:NF025940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET7478657.1"
                     /translation="MTDVREETRPSRNGQVETSHHPETDPQRKDNDYYGIPPIKEHTW
                     TWEIPLYFWLGGIGAGAHVVTTIARLVGVEDQAFLRAGRYTTLVTMLLSPVLLIMDLG
                     RPERFYNMLRIVKWRSPMSMGSWALSVFGGLSGLVATAQAAEDGLLGRDNFAARLAGA
                     IPDRLMSLLALPFGYFVGAYTGVLLATTSVPMWARNAVLMGPTFLSSALSTGLSAISL
                     VLHLGDWGERRTLESLRKAERIALVVESSLLAASLIRMSRWGKPLFSKKLGPLFVGGT
                     LIGGLLAPFALLSGRESRSRSLLASVLVLIGGYLLRYTMVEGGRLSARDPQATFTFAR
                     KENLPLREENV"
     gene            complement(9929..10783)
                     /locus_tag="VFJ72_03980"
     CDS             complement(9929..10783)
                     /locus_tag="VFJ72_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7478658.1"
                     /translation="MATGFLTDTTVCIGCKACEVACKQWNQLPADGYELTGDSYDNTK
                     QLSGTTWRHVAFIENPRKRRLTGDVDLVALAEDVKEQLPGGAEIDALGRWSFMSDVCK
                     HCVEAPCQQACPTGAIIRNEFDNVYIQPDVCNGCGYCVSACPYGVITRVPHDGRAFKC
                     TLCYDRQKDGLEPACAKACPTDSIQFGEVEDLKVRAEERVAELHAKGYTNAYIYGTPE
                     VGGTGGIGGNHSKFILMDEPETYNLPRHPFLPQDKVHQASVASIVTAGVLGAVVAFAF
                     GALERGAR"
     gene            complement(10783..11130)
                     /locus_tag="VFJ72_03985"
     CDS             complement(10783..11130)
                     /locus_tag="VFJ72_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478659.1"
                     /translation="MSDSVRTPRGTRVTPEDTTSSTYRAETSHREMMEQDLMDLGVWL
                     IDHPDAARKNLKAIRSARVLKPGVYYNLGTGMVDRLYSSQRVALGHKMFRISSDPAAP
                     VEEIRRKVLEGKG"
     gene            complement(11127..13664)
                     /locus_tag="VFJ72_03990"
     CDS             complement(11127..13664)
                     /locus_tag="VFJ72_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7478660.1"
                     /translation="MPGLGITYGRGGATSNLQDLQNADCILIEGSNMAEAHPVGFRHP
                     MIAKKKGAKLIHVDPRFTRTSAMCDIYAPLRPGTDIVFLGGLINYVLENERYFEEYVK
                     AFTNAATIITEDFEDANASGLFSGWDEEKKQYNPSSWRYEGMPIDYAAVGGSQGSLLG
                     EAGQGESGEMHPGMERPRDMTLQHERCVFQILKRHFARYTPEMVERVCGTPREAFLEV
                     AETLCENSGREKTSSLCYAVGWTQHSKGVQVIRTAAILWLLLGNIGRPGGGIMALRGH
                     ATIQGSTDIATLYNILPGYLPMPDVNKEHDTWREYVTNETANSGWWSNFPKYITSQMK
                     AWYGEAFDAEKGELFDHFPRLSGDHSYYPTIMEMKDGGVKGAFIFGQNLAVGGPHAKL
                     ARDGLRNLDFLVVLDAYEVETATTWKMDGVKPEECGTETFFIPMALVAEKEGSFTQTQ
                     RMLQWHDKSVEPPGDARSDAWFVYHLGRRMKELYKGSTEPRDSLINAMTWDYPVEGER
                     EEPSIESILKEINGYTVADGKPVGGFAELKDDGSTASGGWIYSGVYANGVNQARRRKP
                     WTEQSQVAPEWGWAWPANRRILYNRASADPDGKPWSERKKYVWWDQDQGKWVGDDVPD
                     FQADKPPSYRPDEDARGVDALPGDGPFILNGDGKGWLFAPIGLKDGPLPVHYEPVESP
                     VPNLIYDKVQHDPVAKTFDRPDNPYNPPEDPRYPYVVTTYRLTEHHTSGAMSRWIPWL
                     SELQPELFCEISPELAAEKGVSNTDWVTISTSRGKIHCKALVSERIPVYKFEGRDLHT
                     IGLPYHWGPNGLVKGDVVNDLIPVSLEPNVAIHEAKAFTANLEAGRV"
     gene            complement(13677..14243)
                     /locus_tag="VFJ72_03995"
     CDS             complement(13677..14243)
                     /locus_tag="VFJ72_03995"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478661.1"
                     /translation="MLFKLTEKDGIWTKIRNHVGMTVKHPISPVTRNIRSRISEEGVE
                     KTVSVCPYCAVGCSQLVYHRDGRIIDIEGNPDSPINAGALCPKGSATFGMHVSPHRKT
                     KVMYRRPYASEWETLSLDEAMDMISARLRKTRDEHWEDKDADGKKLNRSMAVASIGGA
                     TIDNEENYLITKLFHTLGFVRITNQARI"
     gene            14562..15782
                     /gene="fdhA"
                     /locus_tag="VFJ72_04000"
     CDS             14562..15782
                     /gene="fdhA"
                     /locus_tag="VFJ72_04000"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR02819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HET7478662.1"
                     /translation="MADNRVVVYKGPGEVAVEPIDYPKLEVPEEVASAMGMSKEAPHS
                     VILKIVSTNICGSDQHMVRGRTTAPVGQTLGHEITGEVVEKGNDVQFIDVGDICSVPF
                     NIACGRCRNCKERKTGVCLNVNPARAGSAYGYVDMGGWVGGQAEYVTVPFADFNLLKF
                     PDREQALEKILDLTMLSDIFPTGYHGAYSAQVTTGSTVYVAGAGPVGLAAAHSAQLLG
                     AAVVIVGDLNDERLAQARSFGCETINVGEGTPIEEQIAAIVGEPEVDCAVDAVGFEAS
                     AKAGEESPAVVLNQVQTITRAGGSLGIPGLYVTGDPGAADPDAQEGTLKIRFGLGWAK
                     SHAFYTGQCPVMKYNRGLMMSILHDKAQIAKAVNATVISLDEAPQGYKDFDKGAAKKF
                     VIDPHGQLAGAQRN"
     gene            complement(16230..16445)
                     /locus_tag="VFJ72_04005"
     CDS             complement(16230..16445)
                     /locus_tag="VFJ72_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478663.1"
                     /translation="MLRLTGLPNVARYPVATVTRNEDHIVIHFGGRAEEQTMSVPLKY
                     VGGDEEAAELRLLAQLQQIGYTVRRDT"
     gene            16537..17397
                     /gene="fdhD"
                     /locus_tag="VFJ72_04010"
     CDS             16537..17397
                     /gene="fdhD"
                     /locus_tag="VFJ72_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET7478664.1"
                     /translation="MLEKSTGKPRRGSKTKTRVRVVEDGRMRVRPDTLATEEPMEIRL
                     LAGDTKQTVAVTMRTPGSDFDLAAGFLHGEGIISSADEIKKISYCVDSDLDAEQQYNI
                     VNVELRGGRDYDLRPLERHFYTTSACGVCGKASLEQLELRGCPVVPPGPEVSVETIYL
                     LPEKLREAQGLFDATGGLHAAALFDAEGTLVALREDVGRHNATDKLVGWALMERKLPL
                     NDHIVMVSGRSSFEILQKCLTAGVPIVCAISAPSSLAVDVARQFGITLVGFLRDNRFN
                     VYSAPERVCT"
     gene            17586..18785
                     /locus_tag="VFJ72_04015"
     CDS             17586..18785
                     /locus_tag="VFJ72_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET7478665.1"
                     /translation="MQVALGAVYAWSVFQKPLEAHFDASSSTVKLTFSITILALGFAA
                     FAGGLWMAKVGPRMVAMTAGVLYGAGVFLASFAGSSLTLLYLTYGLLGGIGIGLGYIV
                     PVATLIKWFPDKRGFITGVAVAGFGGGAFATALIAKSLVSSVGPFQTFAIFGVIYLIM
                     VVGAALFMKNPPQGWKPEGWEPEEAAGGDKSGIDYDLGGALKTWQWFTLWALLFLNVT
                     AGISIISEAAPMAQEIAGVNATVAAGLVSIISIANALGRFGWAWLSDAIGRKWVFFVM
                     FLLQAALFLILPFAGVYVFLAILSFIIVSCYGGGFGTMPAFTADYFGSANVGKIYGLI
                     LTAWGFGGVLGPLLISYILDTTKSYTLAFYIIAGIMVASAVVAFIIRPPRAESRAESP
                     QAEGARA"
     gene            18966..19301
                     /locus_tag="VFJ72_04020"
     CDS             18966..19301
                     /locus_tag="VFJ72_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HET7478666.1"
                     /translation="MDRNDEWVRVERTEAFKELIRSKRAFIIPATIFFFVFYFGLPFL
                     AAFTTVLDVKVLGSLNLAYLYAFAQFAMTWILMHLYVSRANKWDDLVDRARREASEAT
                     DHVGEGRRA"
     gene            19298..20920
                     /locus_tag="VFJ72_04025"
     CDS             19298..20920
                     /locus_tag="VFJ72_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET7478667.1"
                     /translation="MSDKAIAIIFFVLIIALTLGITAWASRRNKDTAHHYVAGGEIKG
                     WQNGLAIAGDYLSAASFLGIAGAIALTGFAGFYLSIGFLVAYLVVLLLVAEPMRNLGK
                     YTFADMLASRFNTSSVRSVAALSTIAISMFYMIAQLNGAGALIGLLLGFPYALSVIII
                     GVLMTVYIVAGGMVATTWIQIVKAVLLIAGSVTLSIIVLSNFSFNPLALFAAVESEVG
                     NEALIPPPPSSLAASLDVISLNIALVLGTAGLPHILIRFLTVPDAKTARSSIVTATWI
                     IGGFYLLTPILGYGATLFVGRDAIAKANPAGNLAAPQLANELGGPIFFAFVSAVAFAT
                     IVAVVAGLVVAASSAFAHDFYTNVIRHGEATEQEQFRAARYTAVGISVAAIILALFAQ
                     SFNVAFLVALAFAVAASANVPVILLTIFWKKFNTIGAVTGILVGLISSVVLVLISPNV
                     LTGVSPAEGVTPLISVDPLFPLKSPALISVPLGFLGCYLGTVLGGRGAEREREQGLQT
                     SYDEIRVRANTGFSNIEREIRTATPESEPRTT"
     gene            complement(20992..24354)
                     /locus_tag="VFJ72_04030"
     CDS             complement(20992..24354)
                     /locus_tag="VFJ72_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7478668.1"
                     /translation="MRDKRFALLKRSVEDRLLWLGLIVAALYWIIESATDTLVHDETF
                     TERLWPRDPNELWMRFTVVALIVAFSGYAQFFINRQKRIGRSLRDSESRFKGLAEASF
                     EGIVITAEGKVLETNRAFASMFGYDTDEVVGMEAVEFVTAESRELVRRSIFAGSEEPY
                     EVIGLRKDGTCFLAEIRGKMSEYQGRPVRVAAVRDITRSKQIEQELRESEERYRQVFE
                     KNRAVKLLVDPESGDILDANLAAGEYYGYGLEDLKAKNIADLNTLPPDQVHEEMSRAK
                     TDQRGYFIFQHRLSTGEIRDVEVHSSPIEIGGRTVLYSIVHDVTARKAAEEALRVSEE
                     RYRLVSRATSETIWDNNLLTGEQVWNGAAETMLGYDLAGGTSGDWWEKRIHPEDRERT
                     IAGIEEVLREGGEVWSDEYRFRRADGTYLTVVDRGYVVRDSEGRPLRVIGSMLDITER
                     KRAEEELKQSEASLAAAQERAHLGSWEWDRRTNELRWSDEHYRIFGHTPQSFVPSYEA
                     HYLDTIHPEDRERVERAIDEALEGELYSIDYRIVRSDGEIRYVHSHGEALFDGDDRES
                     VGMSGTVQDITERQRAEEDLRRAKEEADEANLAKSEFLANMSHEIRTPMNGVIGMTGL
                     LLDTDLTPQQRKYAQTVRNSGDVLLALLDDILDFSKIEAGEVRIEEIDFDLQAVVKDA
                     ASVFAERAQEKGLELVSFVEYDVPGALRGDPFRIRQVLTNLLSNAVKFTGEGRVALRV
                     EGVEDLGDEVKVRFEVTDTGIGIDEGQQDNLFQPFSQADTSTTRRYGGTGLGLAICKQ
                     LVDLMGGEIGVRSEPGVGSAFFFTLPLKKQPQRVQPAPSSPVTSSVDREDIDEADSEQ
                     SGARVLVVEDTLTNQMVAVELLQRRGYHADVVSNGIEAVEAFSRTSYAAILMDIQMPE
                     MDGYEATAEIRKREGLERHTPIIAMTAHALQGDREKALALGMDDYLSKPVRPEQLDKV
                     LERWSPQAPEPREAGRPATNGASVPGDSLDQSVLADLRLIQREGGGDIVERLVSTFLS
                     ETPAHLDALRKIAGHGEVHDQGDAQDFKRTAHALNGICRGVGANHMASICAELERLGD
                     SGDLTDAPELLSGLEEEFGRVRVLLDAELLRN"
     gene            24504..25052
                     /locus_tag="VFJ72_04035"
     CDS             24504..25052
                     /locus_tag="VFJ72_04035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478669.1"
                     /translation="MLRERFPLLHMFFRYHISMEIEIRHAEPDDYEALHRVFRGPKAI
                     AGTLQLPFPPAETWRKRLSEPGEGSFSLVACVDGELVGELSLWTSPANWRIRHAGGFG
                     MAVRDDWHGKGIGTALLESALELADNWLDLKRVELHVYTDNSAAIALYEKFGFEIEGT
                     HRRFAFRGGEYVDAYSMARLRG"
     gene            25096..25416
                     /locus_tag="VFJ72_04040"
     CDS             25096..25416
                     /locus_tag="VFJ72_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478670.1"
                     /translation="MARAVENGGVCGLCGRMVERRTRHHLIPRSRHGNKRVRRSFARE
                     ELNRTVALCPACHRQIHRTLTEKDLEREYNTVEALLSHPDILRFVRWIERKPHGVIGR
                     GARR"
     gene            complement(25436..25873)
                     /locus_tag="VFJ72_04045"
     CDS             complement(25436..25873)
                     /locus_tag="VFJ72_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HET7478671.1"
                     /translation="MATAERRADVVWKGNLMKGSGTFDLASSGAWTGAEVSFATRTEN
                     PNGNTSPEELIAAAHASCYAMALSNTLAEQGNDPEELDVSANCVLDMGSLKITTVTLD
                     VRGKVPGLDEDGFRSAAEQAEQTCPVSNALRGNVDIQLNTSLA"
     gene            complement(26133..26792)
                     /locus_tag="VFJ72_04050"
     CDS             complement(26133..26792)
                     /locus_tag="VFJ72_04050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET7478672.1"
                     /translation="MARTVEVPEYEVHGEVRVDPARTALIVVDMQNDFVKEGGTLVVP
                     DAEGTIPEIQRLLSLARDSGMRVVYSQDTHTEGDPEWEIWPEHCREDSWGWEIVEDLA
                     PRDDELVIRKVRYDAFYGTHLDHFLRIWDVDTLIICGTVASICVHYTAASAALRWYKV
                     IIPKDATSALDPFDLESSLRQTAFLFNGAITRTGAIQVAGTDEPVYRSKVEEAAEASG
                     D"
     gene            complement(26913..27761)
                     /locus_tag="VFJ72_04055"
     CDS             complement(26913..27761)
                     /locus_tag="VFJ72_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7478673.1"
                     /translation="MRLVYTLLLVAVTAVWGWTFVVVQDAIALYGVLPFLAVRFVLAG
                     ATLAPIYAARLTRRSLLVGGGIGIVLAAGYLFQTTGLLYTTPTNSGLITGLFVVFAPL
                     ADRLLFGASASRQVLVAVFLSLIGMVLLAGGGPEGVNPGDFLTLLCAAALGLHIALLS
                     RYAAGHDAGALTLAQILAMAVLFIIVWPFFDNVSFPPPGVWVALLVTGFLASAGAFLV
                     QTTVQQKIPAARTAIILTMEPVFAAIFGYWLAGDRLVTVQIFGAVLILSALVIGEVLP
                     VLRRGK"
     gene            complement(28320..28937)
                     /locus_tag="VFJ72_04060"
     CDS             complement(28320..28937)
                     /locus_tag="VFJ72_04060"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7478674.1"
                     /translation="MWENLASVLEGETVRLEPLASRHEQGLFEAAGDPRVWRWMPYNA
                     IESSETFRAWFDAALANSEAGTEAAFATLDADTGVPIGSTRYLALRPEHRGLEIGWTW
                     LAPSRWKTGANVEAKLLMLEHAFERLGCMRVEFKTDARNEGSRGALAALPARFEGIFR
                     KHMLVRGGQLRDSAYYSIIDDEWPEVRSNLRGRLKMFQEGPEEAL"
     gene            29112..29909
                     /locus_tag="VFJ72_04065"
     CDS             29112..29909
                     /locus_tag="VFJ72_04065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7478675.1"
                     /translation="MSGYRTADGLIAYRRYGEGTQETVILLHAFPLNSRMWTPQAEAL
                     AGSSRVIAPDYPGFGRSPSSPAQPDVCYYAKCVHRLLDRLGLEKVVLGGISMGGYVAF
                     ECLRQFPGRVSALVLANTRPDPDSEEIRESRREMARRVAAEGVEVLVELQMQRLLAQS
                     TLQNDETTVELVRSMILESNPNGVVAALSAMRERPDSTPLLPEISVPTLVIGGAEDEI
                     STPEVMGEMAAKIPNSRHIVLEGAGHLSNLEAPLEFNAALKVFLKDN"
     gene            29913..30785
                     /locus_tag="VFJ72_04070"
     CDS             29913..30785
                     /locus_tag="VFJ72_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7478676.1"
                     /translation="MDGGRILVVGATGRVGGAAVRYLLEAGFQVRALVRSAQKGTVPR
                     AQGAEVVVGDVTQPATLAPALEGCSGVFSALSAATDRAAVEVEYRGNVNLISAARENG
                     VRRFVYSSAFLVDHPLAQEVGTFREKLRFEQHLLEAQDVSSTILRPVSFMETLLLSMS
                     GPFAFVIGRQNRRAGWISASDIGHAAARAFERDITGRHELAGPDIATFDEAYRHLSRA
                     RGKRIIVLHAPLAAVRAVGQAVAPVEELAGIFTLFDAAGYVSESTALRDTFGVDGISI
                     EEWAGRVSSSGQVG"
     gene            30797..31663
                     /locus_tag="VFJ72_04075"
     CDS             30797..31663
                     /locus_tag="VFJ72_04075"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7478677.1"
                     /translation="MTRLVHVSDLHFGRPAVAERLEGLRELVRKLEPDALAVSGDLTQ
                     RCSNREFRMARQYLDSIGEIAPYLVIPGNHDIRWLGAVARNLGFAGLFQKQAHNFKYS
                     RYTRHISEELSPSLEVPGAVIAALNTAHGITRGSLTNRFKDLGVIGYVRGEDIQLVRE
                     AFENADPDAARIVMIHHNPIEGASSGRHGLSNTEQALQAFADLGAELILCGHDHQEAV
                     HTVERGVRGIVISTAGTISNRLRPGRASSFNLVETDENSLRITPYSWQNPEGFTPSEE
                     SSFPRRATTRSA"
     gene            31754..31969
                     /locus_tag="VFJ72_04080"
     CDS             31754..31969
                     /locus_tag="VFJ72_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478678.1"
                     /translation="MWSRLTRGGDGLPREKVDAIVRLIDQYLAEEATLRNLQSDKQTI
                     HPRDLPPDKREALIAEIFDILKDNRDK"
     gene            32087..34576
                     /locus_tag="VFJ72_04085"
     CDS             32087..34576
                     /locus_tag="VFJ72_04085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7478679.1"
                     /translation="MAETRERRASISIPVTGMSCAACANRVERALSKTGGVSNASVNF
                     ATEKASVEYDPDAVRPEALISAVEDAGYGAEVRETSFGVTGMTCASCVGRVEKAIRKV
                     PGVLDVSVNLATEKASVEYLAGEAEMRDFQKAVEGAGYGVVREEEEASAEDAHEREYA
                     KLRGRFLVAAALTALVLIGSLPMMFSFEAPVPMRWLNLGLLALATPVQFWAGWRFYRG
                     AWGALRHGQANMNTLVAVGTSAAYLYSAVATLAPGLFAGRADVYFDTSSLIVTLILLG
                     RLLEARAKGRTNEAIKKLAGLQARTARVVRDGEEVDIPVEEVEVGDTVVVRPGEKVPV
                     DGRVISGESAVDEAMITGESIPVTKRKGDEVIGATINKTGTFRFTATKVGRDTALAQI
                     MRMVEEAQGSKAPIQRLADRISGVFVPIVMVLATVTFIVWWAFGPEPAFTLALLNFVA
                     VLIIACPCAMGLATPTSIMVGTGRGAERGILVKGGEALEQAHRLTTVVLDKTGTLTRG
                     EPRLTDVIATNGLSEEELLRLAASAERGSEHPLGEAIVRGAKDRGIELANPEDFDAPT
                     GRGVRAAVEGRSVLVGSRKLLDEYGVSEDGLIPEAKEMSADGKTPVFVAVDGEPVGLI
                     AVADTVREESKEAVEALRKMGLKVAMITGDNQRTADAIARELGIDRVLAEVLPDQKSG
                     EVKKLQAEGEIVAMVGDGINDAPALAQADVGIAIGTGTDVAMEAADLTLISGDVRGVA
                     RAIKLSKATIRNIRQNLFWAFAYNVLLIPVAAGVLYPFFAGSAWTVPEALRPFLGEYG
                     FLNPVLAAAAMALSSVTVVSNALRLRRVKIG"
     gene            34588..>34779
                     /locus_tag="VFJ72_04090"
     CDS             34588..>34779
                     /locus_tag="VFJ72_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET7478680.1"
                     /translation="MQDIENASGARHGYIKAHDKEKLQNRLRRIEGQVRGLQRMVDEE
                     VYCIDILTQVASVVSALEKA"
BASE COUNT         6333 a        11013 c        11183 g         6250 t
ORIGIN      
        1 tgagtgaggg tccggtcctg ggatcggcta tgctggccgc cgttggggcg gggatctacc
       61 cggacatcca ggccgccgcc gacaacatgg ttcacaccga gcggaccatc gagccgaacc
      121 tcgaagcgca cgaggcgtac aagttctacc tggaccgcta tatcgagacc tatccggcga
      181 tgaaggagct gatgcacaag acggtccgtc acgaggccag cggccacgag accgcggcaa
      241 aggaagcata ggatgcgtga tcggaggcgt ccacacgaag gcaacgacga gggagcgagt
      301 gaggctgcat gaagattgta tcctgtgcgg gccgtaaggt tcgtttcgat ataaaggagg
      361 cgggaagttg acgaccgtaa cagtcctggg gtcgggcata atggcgacgg cgctcgcgtg
      421 gccgctctcg gacaacggcc acgaggtacg gctggtgggg actcacctcg accgcgagat
      481 cgtggactcc atcaaggaga gcggcgtgca tccgaacctc gacctcaagg tcccggacgg
      541 ggtacgcgcc taccagttgg aagaagccga ggaggctttc gagggcgcgg agatcgtgat
      601 gagcggcgtc aactccttcg gggtgcgctg ggccagcgag cacctggcct cgctcctgaa
      661 gccgggcgtg atggtgatcg ccgtgaccaa gggcatggac gccgatgaag acggcaacct
      721 ccggattctg cctgaagtct tggccgaaca ggtgccccag gacgtgagag atcaggtctc
      781 ctggtcagcg atcgttggtc ctgccatcgc cggcgaggtg gccgtgcgcc acgatacctg
      841 cgtggtcttc gccggcgagg acgcggacgc tttagagaag ctggccgcca ctttccgcac
      901 cgagtactac cacgtatgga ccagcacgga cttcgtcggt tgcgaggttt gcgcggcgac
      961 caagaactgc tacgccttcg gcggcggctt catggaggga ctcctggatc gggagggcaa
     1021 gacggacgcg aggtaccgca ggtacaacta cggcgcggct ctcttcgggc aggcgacgcg
     1081 ggagctcggg cagtggatgg agcttctggg cggagacccg gagacgcccc acgggctgcc
     1141 gggggtcggg gacatgttcg tcaccaccat gggcggacga aacgtcaagg cgggccggtt
     1201 cgtcggcgcg ggcgtgccgt tctcggaggt acgcgacgag aagatgaagg gtgtgacgct
     1261 tgagggggtg gcggccatag aggtgatcgg cggcgcgctg ccgaaactga ccgagcgcgg
     1321 ggtggtcgcc gaggaggatt tcccgctgtt acgacatctt tacgaagtgg tcgccctgga
     1381 cagacccgtg gacatgccct ggagcacgtt cttcggcggc gaggcttaga agctagagag
     1441 aagggataag gctatgaatc gcgagagttt gctgcaacac gtcggagagc gtccgttcga
     1501 cctaatcgtg atcggcgccg gcatcaacgg cgccggcatc gcccgcgacg ccgccatgcg
     1561 gggcctgcgg gtgctgatgc tcgacaaggg cgacatcgca agcgggacca cccagtgggc
     1621 gacacggctc atccacggcg gcctgcgtta cctggagcac tacgaggtgc cgctggtgcg
     1681 cgagtcgcta gccgaccggg agatactact tcacatagcg ccgcacctgg tcaagccgct
     1741 caagttcacc gtgccgatct atgaccgctc cagccgcggg cccggcatga ttcgtctggg
     1801 catgatcggc tacgacacgc tctcttacga ctccagcatg ccccgccaca agatgctctc
     1861 ccgcgaggag gccctgcgcg agtacccggg cctgaacccc gaaggactca aggcggcggc
     1921 cacctactac gatggacagg tcgagtacgc cgagcgtata gcggtggaga acgcggtttc
     1981 ggccgttcag aatggcgcgg tggtgatcac ctacgccgag gtgaagcggt tgctcttcga
     2041 ggacggcggc tcccgcgtag cgggggtgga gttcgaggat aagctggagg gcggcgtgta
     2101 tagcgtccgc gcgccggtga cggtgaacgt ggccgggccg tgggtggata gggtcctggg
     2161 cggctccggg gccgtcagcg tcgagacgcc agggaacggc gcgaatgggg attccggggg
     2221 catgatcggg ggcacgaagg gctcgcacat catcgtggac ccgttccccg gagcgccgaa
     2281 gaacgccatc tacgtcgagg cgaggaagga tgggcggccg tacttcatcg tgccgtggaa
     2341 cgagcgctac ctcatcggca cgaccgacct gcgctacagg gacgacctcg actacgtgtc
     2401 tgcgagtgag gaggagatcc agtacctcat agacgagacg aactacgtga tcccggatgc
     2461 gcacctgacg cgggactcgg tgctcttcac ctactctggg gtgaggccgc tgccctccgc
     2521 gcccgcgggc tccgagggct ccatcacgcg ggcccacgtc gtctacgacc acgcgaaggg
     2581 taagtcggtg gccgggggga ggatcaaggt ctccggcggt ggcggtccac gcgccgaggg
     2641 tctgctctcc atcgtcggcg gcaagctgac gacctaccga aacctcggcc gtcaggcgac
     2701 gaacgtcgtc tacaagaagc tcggggccaa gcctcccaag agcgcgacgg acaggatccc
     2761 gcttcccggc ggacggaccc cggacttcgc cgctttcacc gaggagttca ggagcaggag
     2821 cggcctcccc gacgtgctcg cccggcggct gatcaggctc tacggagccc gcgcctacga
     2881 cgtgctcgcc gaggccgggg acgactcctc tctcagggaa ccgctcgtct ctccgccagc
     2941 cgtggagacc ggcctgatgg gttgcgaggt cctctacgcc ttccgccagg agatggcgca
     3001 gaagctctca gacgtgctgc tccggcggac gatggtcgcg tacggtccgc aggtcgccct
     3061 ggacgttgac gaggccgcgg ccgcggtcgc ggtcaagcac ctcggatgga gcgaggagcg
     3121 cgctaagaaa gaagtcgccg agtaccggga gtggatccag cgctacacgc ccaaagcgct
     3181 ccgggagcgc gagggagccg gggctccggg aagcgcggag tagaggtcgt gcgctccgcg
     3241 aggttacgcg ccgcgcaccg gttcggggga ggtctggcat gatctccgcc gtacttttcg
     3301 acctggacgg tacgttgttg gagacggagg aattgaaggg cctctcatac gcccgttccg
     3361 tcaccgagct acgccccgat gtcgcggaag aagacgtgat gtccgcctac gccgacgacc
     3421 tggtgggaca ctcgcgccag gaggtcgcca cggacctcat gcgacgcttt cagttggagg
     3481 aggcggccag gggacgtatg gatgagttcg gagaggaaga gccgtggcgt gtgctcgtga
     3541 agatccgtct cggcgtctac gaggcgctgc tagaagactc cgacctgctc ctcgaaaagc
     3601 gatacccgca caacatagag atgctgcggg agttgcggcg cgaaggctac ccgacggcgt
     3661 gcgccaccat gtcccaccgt taccaggtcg agcgcgtgct ctccgtgctc gacctcgaag
     3721 acgccttcga cgtggtggcg accgcggacg acatcaagcg tggcaagccc gacccggaga
     3781 tagacctgct cgtcgcggag aagatgggcg tgccgccgga ggagttcctc gtaatagagg
     3841 attctcccgc cggggtgagg gcggccctgg cagccgggat gatggtcgtt gccgtgccga
     3901 ccaagctaac cgtgcgcggg gtgcgcgcag cgggcatact ggagccgcgc tgggtggttg
     3961 aggaccccga aatgctgccc cgcgtagtgc gccagcgtat agaagaggcc gggggaaggt
     4021 cggaaggggg ctgatgactg gaaatcccac aagctcatca gcggactcgc aacctgtacc
     4081 gcgccgttcc ggcgccgggc tggaagcaga ccgcttcgaa cgcgacgata tgaagacggt
     4141 ataaagcaag ggaccacgcc gcctggaaca taggaggtca gaacatgcga atcgttcagt
     4201 tgtcggacat acatacggga acgccgctct tccggccgga cttcctgcag gcggcgatag
     4261 aggagaccaa cgcgcttgag ccggacctgg tggccgtggc cggggacctc accacagagg
     4321 gatacaggtg ggagttcgag gaggcaaaag gatacctgga ccgcatagag tgcgagaacg
     4381 tcgtcgtgat catgggcaac cacgacgcca agaacgtcgg ctaccggcat ttcgaagact
     4441 tcttcggtcc ccggatgcgc gcgatgacgg tccccatccc ggacggcgag gcaaaggtcg
     4501 tggcgctcga ctccagcaag ccggacctcg ccgagggtga gatcggccgc gagcactacc
     4561 cctggatcga ctccgagttc cggggctggg acaggggacc aaagattctc gtcgtccacc
     4621 atcacatcct cgccgtcccc ggcacgggac gcgaccggaa caacctgcgc gactcgggag
     4681 acataatggc cgtgttgcgc gagctggaag tagatatggt cctcgccgga caccggcacg
     4741 ttccatatgt ctggagcatc tccggggtga ggatcatcca ctccggcacg gtgtcgagca
     4801 tgcgggtccg cgggatcatc cccccctcct acaacgtaat agatttcggt cccgacaagg
     4861 tcgaggtaac gctgcggcat ccgggcggcg agcaggagcc gctcgccagg ttctcgcgcc
     4921 gggcggtcac gaccagcgag atctaccccg aactcgaccg cttcgtccgt tacgacgaag
     4981 tgcccgtgta gagcaaccgt ctgtatgaag atcattccgg gccggtccga aagaggagga
     5041 gataatgcct agagagatcg gcgatctgcc gcacgccata gagaacgagg gcgagctgga
     5101 gcagcgcctg gccgggaagc gcctcgccgt cttcctcgac tacgacgggg tgctcactcc
     5161 tatcgttgac cgcccggagg acgcgatcat ctccgagggc atgcgggagg ttatcagcaa
     5221 cctcgcggag cgttgtcccg tgtgcgtggt cagcgggcgg gaccgccagg tggtgcaaca
     5281 actcatgggc ctcgaaaacc tgatcgtcgc cggcagccac ggcttcgata tctggagtcc
     5341 cggcgagggg acgatccagc acgacgcggg cgccgggttc gaggacctgc tcgaagacgt
     5401 cagggactac ctgcagagtg aggccgctcc gatagaaggc tcctccatcg agacgaaaaa
     5461 ggcttcggtg gcgatccact accgcctcgt cgcggaagag gagcgtccga agatcaagga
     5521 gatagtagac agcctgctcg aagagcgtcc cgacgagttg aaagtaacac ccggaaagat
     5581 ggtctacgag gtccagccga agatagactg gcacaagggc aaggcggtcc tctatttgct
     5641 ggaagccctg gatctggacg gcgacgacgt gatgaccatg tacctcggtg acgacgtgac
     5701 ggacgagaac gccttcgaag ctctcaacga gacctcgccg aaggggatag gcatcttcgt
     5761 cggcgaggcg gaccacccgg aggtcgtagg gcggaccacc tcagcggact acatcctgta
     5821 cactgtgccg gaggtggaga cgtttctgga tgggctggcg cggtaacggc tgggcgaggg
     5881 ccgatgacgg gcggcgcgga agaagccaac gtatagggga tattgtgtac cggcttccat
     5941 caaggcaaca gaagcaggta aacggtaggg gtgggcccgg tggccgggct gcaaacggga
     6001 aatatcaggg tgaagatcgg gtagaacggt gacgtttcgt ctcacggttg accatgaggg
     6061 acagggagga caggcatgac gaactggacg ctttcctacg actactacga cccggaggaa
     6121 gagggactgc gcgaggcgat gtcctcgacc ggcaacggct acttctgcac gcggggcact
     6181 gcccactggg ctgacgcgga ctcggttaac tatccgggca cctacgtcca cggcggcttc
     6241 aaccgcgaga ctacgatcat ggccggaagg cccgttctga acgaggattt agtcaacctg
     6301 cccaactggc tcgtcctgaa gctccgtatc gaaggggacg gagagcccat agacttcggc
     6361 aatgtcgaga tcctctcgta ccaacacgag tacgatatac gcaacgcgac cctgacgcgc
     6421 aacctcaggt tccgggaccg ggaagggcgc gagacatctc tggagagccg gcgcttcgtg
     6481 agcatggcag acatgcacca ggcggctatc gagtggaaga tcacgccgga gaactggtcg
     6541 ggcaaggtgg agataatcac ggcggtggac ggccgggtca ccaacggagg ggtcgcccgc
     6601 tacaaagagc tggaaggccg ccatctggac ccggtatcgc cccggacgtt cgggcccgag
     6661 atcatcgccc tgctggtgcg tacccgccag tcgcgcatct acgtagccga ggcggcgcgc
     6721 acgcgcgtct acggagagaa tggcatgatc tcccttgacc gcgaccttca ccagatgcag
     6781 gactacatac atcaggtcct gggcttcgac gtagaggaga agtcgactgt ccgggtggag
     6841 aagatggtct ccttctacac ctcgcgcgac cacgccataa acgagccgct gtccaacgcc
     6901 gggcgggcgg tcatgcgcta cgggacgttc gaggaggcct tcgagcagca cgcgcgcgtc
     6961 tgggatgagt tgtgggacgc ctgcgacatg aagatcccgg gcgacgaccg ggtacagctc
     7021 ctggtgcgcc tgcacatctc gcacatcctg caggtctgct ccccgcacac cgccgacctc
     7081 gacgccgggg ctcccgcccg cggactcaac ggcgaggcct accgcggaca catcttctgg
     7141 gacgagctct acatctaccc cttcctgaac ttccggctgc cccagatcac gcgcgagcta
     7201 ctcgtgtacc gttaccggcg cctcggcgaa gcccgcgcgg cggccaagga ggtcggctac
     7261 aggggagcga tgtacccgtg gcagagcggc tccgacggct cggaggagac ccagaaagtg
     7321 cacctgaacc ccaactccgg gaactgggac cccgacctca gccaccggca acggcatgtc
     7381 ggcgcggcca tcttctacaa catctggcac tattaccagg tgacgggaga catggagttc
     7441 ttgatagacc acggggcgga gatgatgctg gagatcgccc gcttctggtc atccatcgcc
     7501 cactacaacg atgagcggga ccggtacgag atccacggcg tcatggggcc ggacgagttc
     7561 cacgagaagt atcccggttc cgaggaggaa ggactcagaa acaacgccta cacgaacgtg
     7621 atggtcgcct ggatctgcga gagcgcccag gaggtcttga agatgctccc cgagaagcgc
     7681 cgggattccc tccggtcaaa gatcgggctc tccgacgacg aggtggagct gtggggcgag
     7741 atgagtcaca agatgttcgt cccgttccac gacgacggca tcatcagcca gttcgagggc
     7801 tatgagaacc ttgaggagct tgactgggac gcctaccggg agaagtacga aaacatccag
     7861 cgcctcgacc gcatcctgaa ggccgagggc gacgacccgg accgctacaa gctagccaag
     7921 caggccgata ccttgatgct gttcttcctg ttcccggagg accagttgaa gcggctgttc
     7981 gagcggctcg gctacgacta caagcccgat acggcccgca agaacatcga gtactatgcg
     8041 cagcgcacat cccacggctc caccctgagc ttcatcgttc acgcggccat catggccgac
     8101 ctcgacccgg agagttcgtg gaaacggtac atggtcgcgc tggagagcga tatcggggac
     8161 gtgcagggcg gcacgacgcg agaggcgatc cacatgggcg tgatggccgg cacgctggac
     8221 ctcctgcagc ggggatacat ggggaccgag atccgggacg gcgtcgtcca cttcgcgccg
     8281 aaactcatca agaagctcga agggttgtcg ttcccgatgc gtttccgcga gactcccatg
     8341 gagattacac tagaatccgg caagctggcg gtggcggcgc actccgacgg cctgaaccag
     8401 tgcatcaaag tcggcgttgg tgacacggtc caggagataa gagccggcga gagccatacg
     8461 ttcagcatct agccacattc ggacagaagc aaagcggcag cctgcggata actttggcag
     8521 gctgccgccg aggatcgggc gttaaagagg agcaaaccat gagggtaatc accgcgaaag
     8581 acatgcagac cgggcgcggc cccgacgact ggtcccgaag gtcagatcct gcacgccatc
     8641 accggcacct gtcgcgccgc aaaggaaggt gaatcgcccg tcgaagtcgg ccccggagga
     8701 gtcctctact tcgcccctga caggaaacac tggcacggcg ccacctccga gacttacatg
     8761 atccacctcg ccgtcaacgt cgccaccacc accgacggcg gcaccgactg gctggagccc
     8821 gtcacggacg agcagtacgg cgtctgaccg gaacgctaat ccgccaatct attcgggccg
     8881 ggatctctcc tgatttagac gttctcctcc cgcagaggca ggttctcttt acgggcgaaa
     8941 gtgaaggttg cctgcggatc gcgggcggag agtcgtccgc cttcgaccat ggtgtagcgc
     9001 agcaggtatc cgccgatcag gaccaggacc gaggccagca ggcttctgga gcggctttcg
     9061 cgtccggaga gtagcgcgaa cggggcgagg agccccccga tgagagtgcc gcccacgaag
     9121 agcggcccga gcttcttcga gaagagcggc ttcccccacc ggctcatccg gatcagggac
     9181 gcggccagaa gactgctctc gacgaccagc gcaatccgct ccgccttccg cagggactcc
     9241 agcgtcctcc gttcccccca gtcaccgagg tgcaggacga gggagatggc cgagagcccc
     9301 gtggagagcg ccgaggacag gaaggtcggt cccatcaaga cggcgttgcg ggcccacatc
     9361 ggcaccgaag tcgtcgccag gagcacgccc gtatacgcgc cgacgaagta cccgaacggg
     9421 agagccagca gggacatgag ccgatccggg atggctcccg cgagccgggc cgcgaagttg
     9481 tcgcgtccga ggaggccgtc ctccgcggcc tgcgcggtgg cgaccaggcc gctcaacccg
     9541 ccgaacaccg ataaggccca cgaacccatg ctcatcggcg agcgccactt cactatgcgc
     9601 agcatgttgt agaagcgctc cgggcgtccg aggtccatga tcaggagtac cgggctcaga
     9661 agcatcgtca ccagcgtcgt gtagcggccc gcgcgcagga acgcctggtc ttcgacgccc
     9721 accaggcggg ctatggtcgt gacgacgtgg gccccggccc ctatcccgcc gagccagaag
     9781 tacaacggga tctcccacgt ccaggtgtgc tcttttatgg gagggatgcc gtagtagtca
     9841 ttgtcttttc tctgcggatc ggtctccggg tgatgggagg tctcgacctg accgttgcgc
     9901 gacgggcgag tctcttctct aacgtccgtc atctggctcc cctctctagc gctccgaacg
     9961 cgaacgccac gaccgctccg aggaccccgg cggtgacgat gctcgcgacg ctggcctggt
    10021 ggactttgtc ctgcggcagg aacgggtggc gcggcaggtt gtacgtctcc ggctcgtcca
    10081 tgaggatgaa cttggagtgg ttgcccccga taccgccggt acctccgacc tcgggcgtgc
    10141 cgtagatata cgcattcgtg tagcccttcg cgtgaagctc cgccacgcgc tcctcggccc
    10201 tgacctttag atcttcgacc tcgccgaact ggatggagtc ggtcgggcag gccttggcgc
    10261 aggccggctc cagaccgtcc ttctgccggt cgtagcagag cgtgcatttg aaagcccgtc
    10321 cgtcgtgcgg gacgcgggtt atgacgccgt aggggcaggc cgagacgcag tagccgcagc
    10381 cgttgcagac atcgggctgg atgtacacgt tatcgaactc gttgcggatg atcgcgcccg
    10441 tcggacacgc ctgctggcac ggcgcttcta cgcagtgctt gcacacgtcg ctcatgaacg
    10501 accagcgccc cagagcgtcg atctccgcgc cgcccggtag ctgctccttc acgtcctcgg
    10561 cgagagcgac gaggtccacg tccccggtta gacggcgctt gcgcgggttc tcgatgaagg
    10621 cgacgtgtcg ccaggtggtg ccggagagtt gcttggtgtt gtcgtaggag tcgccggtca
    10681 gttcgtagcc gtcggcgggg agctggttcc actgcttgca ggcgacctcg caagccttgc
    10741 acccgataca gaccgtcgtg tcggtcagga atcctgtagc cactagccct tcccttctag
    10801 tactttgcgc cggatctctt cgacgggcgc cgccggatcg ctgctgatgc ggaacatctt
    10861 gtgacccagg gcgacacgct gggaagagta gagcctgtcc accatccccg tccccaggtt
    10921 gtagtagacg ccgggtttga ggaccctggc gctccttatg gccttcaggt tcttcctggc
    10981 ggcatcggga tggtcgatga gccacacccc gaggtccatc aggtcttgct ccatcatctc
    11041 ccggtgcgag gtctcggccc ggtaggtaga gctggtcgta tcctccgggg taacgcgcgt
    11101 gcctctcggg gtcctcacag agtcgctcac acgcgccccg cttcgaggtt cgcggtaaac
    11161 gctttggctt cgtggatggc gacgttcggt tcgagggaga ccgggatcag gtcgttcacc
    11221 acgtcgccct tgacgagccc gttcggtccc cagtgatacg gaagtccgat ggtgtgaaga
    11281 tccctgccct cgaacttgta gaccgggatg cgctcggaga cgagggcctt gcagtgaatc
    11341 ttgccgcggc tcgtcgagat ggtaacccag tcggtgttgc tcacgccctt ctcggcggcg
    11401 agctccgggc tgatctcgca gaaaagctcc ggctgaagct cggagagcca ggggatccaa
    11461 cgactcatag ccccactggt gtgatgctcg gtgagccggt aggtcgtgac cacgtatggg
    11521 taacgcgggt cctcgggcgg gttgtacggg ttgtcgggac ggtcgaaggt cttggctacg
    11581 gggtcgtgtt ggaccttgtc gtagatcaag ttcggcaccg gcgactctac cggctcgtaa
    11641 tgtaccggca atggcccgtc cttgagccct atcggtgcga agagccagcc cttgccgtcg
    11701 ccgttgagga tgaacggccc gtctccgggg agggcgtcca ccccgcgggc gtcttcgtcc
    11761 gggcggtagg aaggcggctt gtcagcctgg aagtccggca cgtcatcgcc gacccacttg
    11821 ccctgatcct ggtcccacca gacgtatttt ttgcgttcgg accagggctt gccatccggg
    11881 tcggcggagg cgcggttgta gaggatgcgg cggttcgcgg gccacgccca gccccactcc
    11941 ggtgctacct ggctctgctc ggtccagggc ttgcggcggc gggcctggtt cacgccgttc
    12001 gcgtagacgc ccgagtagat ccaaccgcca ctcgccgtag aaccgtcgtc ctttagctcc
    12061 gcgaaaccgc ccaccggctt gccatcggcc acggtgtagc cgttgatctc cttgaggatg
    12121 ctctcgatgc tcggctcctc ccgctcgccc tcgaccgggt agtcccacgt catcgcattg
    12181 atgagcgagt cgcgcggctc ggtagatccc ttataaagct ccttcatcct gcgccccagg
    12241 tggtagacga accaggcgtc cgagcgggcg tcgcccggcg gctcgacgga cttgtcgtgc
    12301 cactggagca tccgctgggt ctgggtgaac gaaccctcct tctccgcgac cagcgccatc
    12361 gggatgaaga aggtctccgt gccgcactcc tccggcttca caccgtccat cttccaggtg
    12421 gtcgccgtct cgacctcgta ggcgtcgagc acgacgagga agtcgaggtt ccgcaggccg
    12481 tcacgcgcca gcttcgcgtg cgggccgccg accgcgaggt tctgcccgaa gatgaaggcc
    12541 cccttcaccc cgccgtcttt catctccatg atcgtcggat agtaggagtg atctcccgat
    12601 aggcgcggga agtggtcgaa cagctcgccc ttctccgcgt cgaacgcctc gccgtaccag
    12661 gccttcatct ggctcgttat gtacttcggg aagttcgacc accagccgct gttggcggtc
    12721 tcgttcgtca cgtactcgcg ccaggtatcg tgctccttgt tcacgtccgg catcggcagg
    12781 tatccgggga ggatgttgta gagggtggcg atgtccgtgg agccctggat ggtggcgtgg
    12841 ccccggagcg ccattatgcc gccgcccgga cgtccgatgt tgccgagcag gagccacagt
    12901 atggccgccg tgcggatgac ctgcacgccc ttggagtgct gggtccagcc gaccgcgtag
    12961 cacagcgacg aggtcttctc gcgaccggag ttctcgcaga gcgtctcggc gacttcgagg
    13021 aacgcctccc tcggcgtccc gcagacccgc tcgaccatct ccggcgtgta gcgggcgaag
    13081 tggcgcttga ggatctggaa gacgcagcgt tcgtgctgga gcgtcatgtc cctgggacgc
    13141 tccatgccgg ggtgcatctc cccgctctcg ccctgtccgg cctcacccag taggctgccc
    13201 tggctcccgc ccacggcggc gtagtcaatc ggcatcccct cgtagcgcca actcgacgga
    13261 ttgtactgtt tcttctcctc gtcccagccg gagaatagcc ccgaggcgtt cgcgtcctcg
    13321 aagtcctcgg tgatgatcgt ggcggcgttc gtgaacgcct tgacgtactc ctcgaaatac
    13381 ctctcgttct ccagcacgta gtttatgaga ccgcccagga acacgatgtc cgtgccgggc
    13441 ctgagaggcg cgtagatgtc gcacatcgcc gaggtgcggg tgaagcgcgg gtccacgtgg
    13501 atgagcttcg cgcctttctt cttggcgatc atggggtggc ggaagccgac cggatgagcc
    13561 tcggccatgt tggagccttc gatcaggatg cagtcggcgt tctgcaggtc ctggaggttg
    13621 gaggtggctc cgcccctacc gtacgtgatg cccagaccgg gcaccgtgga gctgtgtcat
    13681 attcgcgcct ggttcgtaat tcgcacgaag cctagagtgt ggaagagctt ggttatgagg
    13741 tagttctcct cattgtctat cgtcgcgccg ccgatggagg cgacggccat cgagcggttg
    13801 agcttcttgc cgtcggcgtc tttgtcctcc cagtgctcgt cgcgggtctt cctgaggcga
    13861 gcggagatca tgtccatcgc ctcgtccagc gagagcgtct cccactcgct cgcatacggc
    13921 cggcggtaca tgaccttcgt cttgcggtgc ggcgagacgt gcatcccgaa cgtcgccgag
    13981 cccttcggac agagcgctcc ggcgtttatg gggctgtccg ggttgccctc gatgtctatg
    14041 atgcgcccat cgcggtggta gacgagttgc gagcagccaa ccgcgcagta cggacagacg
    14101 ctcacggtct tctccacgcc ctcctcggag atgcgcgacc ggatgttgcg cgtcaccggg
    14161 cttatcgggt gcttcaccgt catccctaca tggttccgga tcttcgtcca gataccatct
    14221 ttctccgtga gcttgaagag catgcctgtc cccttctgcg cgaacgattc tgttgtacgg
    14281 atttccctct accaattcta accgtattca ccctggcgcg aaaccacagg ataacttacg
    14341 atccagatat gtttgctgcc cggatatata gccgtaacct tgtgtcgcaa cgcaacatat
    14401 ggtactttgc tggggcagct ttcggggaag ggaaaggagg ttgcctggat atctgagatt
    14461 ctgctgcgcc ggaggttagt aatttttcgg cgcgtgctgg cagagacaag cataagcaaa
    14521 cttcaccatc tacagctaga cagaaggaga aactccagct tatggctgac aacagggttg
    14581 tggtctacaa gggaccgggc gaggtagcgg tagagccgat cgattatccg aagctcgaag
    14641 ttcccgaaga agtagcgagc gcgatgggga tgagcaagga ggctccgcac tccgtcatcc
    14701 tcaagatcgt ttcgaccaac atctgcggct cggaccagca catggtccgc ggtaggacga
    14761 cggctccggt cgggcagacg ctcgggcacg agatcacggg cgaggtcgtc gagaagggca
    14821 acgacgtcca gttcatcgac gtgggcgaca tctgctcggt gccgttcaac atcgcctgtg
    14881 ggcgctgccg caactgcaag gagcgcaaga cgggcgtatg tctgaacgtg aacccggcgc
    14941 gagccggttc cgcttacgga tacgtggaca tgggcggctg ggtcggcggg caggccgagt
    15001 atgtgacggt tccgttcgcg gacttcaatt tgctcaagtt cccggacagg gagcaggcgc
    15061 tggagaagat cctggacctc accatgctct ccgacatctt ccccaccggc tatcacggcg
    15121 cctactcggc ccaggtaacg accggctcga cggtctacgt cgcgggcgcg ggtccggtgg
    15181 gcctggccgc ggcccactcg gcgcagctac tcggagcggc ggtcgtcatc gtcggcgacc
    15241 tgaacgacga gcgtctggcg caggcccgca gcttcggctg cgagacgatc aacgtcggcg
    15301 agggcacacc catagaggag cagatcgccg ccatcgtcgg cgagcctgag gttgactgcg
    15361 ccgttgacgc ggtcggcttc gaggcctccg cgaaggccgg cgaagagtcc ccggcggtgg
    15421 tcctcaacca ggtccagacc atcacgcgcg cgggcggctc tctcggcatt ccgggtctgt
    15481 acgtcaccgg cgaccccggc gccgccgacc cggacgctca ggagggtacg ctcaagatcc
    15541 ggttcggcct cggctgggcc aagagccacg ctttctacac gggtcagtgc ccggtcatga
    15601 agtacaaccg cggtcttatg atgagcatcc tgcacgataa ggcccagatc gccaaggccg
    15661 tcaacgccac ggtcatctcc ctcgacgaag ccccgcaagg ctacaaggac ttcgacaagg
    15721 gcgcggccaa gaagttcgtc atcgatccac acggtcagtt ggcaggcgct cagaggaact
    15781 aaaacttcgg caaagcagga tggagagggg ccggagagat ccggcccttc ctcttgccgg
    15841 tataattcgt agcatggtct agccggactc ttgctttcgc attcagaacg aggagcagat
    15901 acgcggtaag gaccgtatag acgtggaccc gccccggacc cggtgatcga gatagacatc
    15961 accagccctt ctttcagcaa gctttagcaa gctcccgatc tatgctcaga taggcgtccc
    16021 cgaggtctgg cgctacgacg gagagcgcct gaagatcctc gtcctcgaag atgtcggata
    16081 cgtcgagacc cacgaaagca ccgctctgcc atccgtcact agcgtcgttc tcgcgaactt
    16141 cgcagagaag agagcaagac aacgaagcgc acggtctggc tcgaatcggt gcgcgagtgg
    16201 gcgcgaggag agtccgggaa cgctacctct caagtgtctc gccggaccgt gtagccgatc
    16261 tgctggagtt gagcgaggag ccgcagctcc gctgcttcct cgtcgccgcc gacgtacttt
    16321 agtgggacgc tcatcgtctg ctcctccgcc cgtccgccga agtggatgac gatgtgatcc
    16381 tcgttgcgcg tcacggtggc gacgggatag cgagcgacgt tggggaggcc cgtgagcctc
    16441 agcatggcga ctggctccga atagtcatgg aagcatacta gcatgctcgt gctgatacac
    16501 tttcccgcac agctcaaaca cggagcggag agacggatgc tggagaaatc cacgggcaag
    16561 ccccggcgcg ggagcaagac gaagacgcgg gtgcgggtcg tcgaggacgg ccggatgcgc
    16621 gtgcggccgg acacgctcgc gaccgaggag ccgatggaga tccggcttct ggccggcgat
    16681 acaaagcaga ccgtcgccgt caccatgcgt actccagggt cggacttcga tctggcggcg
    16741 ggcttccttc atggcgaagg catcatctct tccgccgacg agataaagaa gatcagctac
    16801 tgtgtggact ccgacctcga cgccgagcag cagtacaaca tcgtcaacgt ggagctgcgc
    16861 ggcggccgcg actacgacct gcgtccgctg gagcgtcact tctacaccac gagcgcctgc
    16921 ggcgtgtgcg gcaaggcgag tttggagcag ttggaactgc gcggctgccc ggtcgtccct
    16981 cctggaccgg aagtatccgt ggagaccatc tacttgctgc cggagaagct cagggaggcg
    17041 cagggactct tcgacgctac gggcggactg catgcggcgg cgctcttcga cgccgaagga
    17101 acgctggtcg cgctccggga ggacgtgggc cgccacaacg ctaccgacaa gctcgtcggg
    17161 tgggcgttga tggagaggaa gctgccgttg aacgatcata tcgtcatggt cagcgggcgg
    17221 agcagcttcg agatcctcca gaagtgtctg acagcgggag tgcccatcgt ctgcgcgatt
    17281 tcggcgccga gcagcctcgc cgttgacgtg gcgcggcagt tcggcatcac gctcgtgggg
    17341 ttcttgcgag ataaccgctt taacgtatat agtgcgccgg agagggtttg tacctgatcc
    17401 tggacgggga tgttcctcgc aatgtcagcc cgtcttggtg gggaggtgag agaagaatac
    17461 tgctccggta acggtacgga tgggtgcgta atttgttttc gggaaagggg atttagatgt
    17521 cgagtcgagc agccagtaac tctggcgcgg gtaacagagt actcatagcc atagcaggcg
    17581 tagtcatgca ggtggcactt ggagcggtct acgcctggag cgtgttccag aagccgctgg
    17641 aagcccactt cgatgcgtca tcgtccacgg tcaagctgac cttcagcatc acgatcctgg
    17701 cgctcggctt cgcggccttc gcgggcggtc tatggatggc taaggtggga ccgcgcatgg
    17761 tggcgatgac ggccggggta ctctacgggg ccggggtttt cctggcgagt ttcgccggga
    17821 gcagcctcac gctgctctac ctgacctacg gcctgctcgg cggtataggt ataggtctcg
    17881 gctacatcgt gccggtcgcc accctgatca agtggttccc cgacaagcgg ggcttcatta
    17941 ccggtgtcgc cgtagccgga ttcgggggtg gagcgttcgc tacggcattg atagcgaagt
    18001 cgctcgtatc ctcggtcggc ccgttccaga ctttcgctat cttcggcgtc atctacctca
    18061 tcatggtcgt cggcgcggcg ctgttcatga agaacccgcc ccagggttgg aagcccgaag
    18121 gttgggagcc cgaagaagcc gcgggcggcg acaagtcggg gatcgactac gacctcggcg
    18181 gcgccctgaa gacctggcag tggttcactc tgtgggcgct cctgttcctg aacgtcaccg
    18241 ccggcatctc cataatctcc gaggccgcgc cgatggcgca ggagatcgcc ggcgtcaacg
    18301 cgaccgtcgc cgccggcctg gtcagcatca tctctatcgc taacgccctc ggacgattcg
    18361 ggtgggcctg gctctcggac gccataggcc gcaagtgggt gttcttcgtg atgttcctgt
    18421 tgcaggccgc gctcttcctg atcctgccgt tcgccggagt ctacgtcttc ctggctatcc
    18481 tctcgttcat catagtgtcg tgctacggcg gtggtttcgg gacgatgccg gccttcaccg
    18541 cggactactt cggctcggcg aacgtcggca agatctacgg cctcatcctt accgcctggg
    18601 gattcggcgg cgtactggga ccgctactga tctcctacat cctcgacacg acgaagagct
    18661 acacgctggc cttctatatc atcgccggca tcatggtagc gagcgccgtg gtcgcgttta
    18721 ttattcgtcc cccgagagcg gaatcccgcg ccgagtctcc gcaagcggaa ggggcccgcg
    18781 cctgacaatg cctgtctcac aggcgtaagg tttcataagg tttggagccc tccgttctag
    18841 cgcggagggc tttttcttga ctggatcccg caccctctca cgccattcat atatggttga
    18901 ctgtatacaa cacatgccat atagtgatca atcggtcgga ggaaactcaa gggaaagggg
    18961 aataagtgga caggaacgac gagtgggttc gtgtggagcg aaccgaggct ttcaaggagc
    19021 tcatacgaag taagagagca ttcatcatac cggcgacgat cttcttcttc gtgttctact
    19081 tcgggttgcc gtttctggca gcgttcacaa cagtgctgga cgtcaaggtc ctgggttcgc
    19141 taaacctggc ttatctctat gcgttcgcgc agttcgcgat gacctggatc ctgatgcacc
    19201 tctacgttag ccgggcgaac aagtgggacg acctcgtgga tcgcgcgcgg cgcgaggcca
    19261 gcgaggctac ggaccatgtc ggtgaaggga gaagagcgtg agcgataagg caatagccat
    19321 tatcttcttc gtcttgatca tcgcgcttac gctcggcatc acggcgtggg cctcccggcg
    19381 gaacaaggac accgctcacc actacgtcgc gggcggtgag atcaagggct ggcagaacgg
    19441 gctcgctatc gccggcgact acctctcggc ggcctcgttt ctcgggatag ctggcgctat
    19501 cgccctgacc ggcttcgcgg gtttctacct ctcgataggc ttcctggtag cgtacctggt
    19561 ggtgctcctg ctcgtggccg agccgatgcg caacctcggc aagtacacct tcgcggacat
    19621 gctcgcgagc cgcttcaaca cgtcgagcgt gcgctccgtg gcggcgctca gcaccatagc
    19681 gataagcatg ttttacatga tcgcgcagct aaacggcgcg ggggcactga tcgggctcct
    19741 gctcggcttc ccctacgccc tctcggtcat catcatcggc gtcctgatga ccgtctacat
    19801 cgtcgcgggc ggcatggtgg cgacgacctg gatccagata gtcaaggccg tgcttctgat
    19861 cgccggctcc gtgaccctct cgatcatcgt cctgtcgaac ttcagcttca acccgctggc
    19921 gctcttcgca gcggtcgaga gcgaggtcgg taacgaggcg ctcatcccgc cgccgccgag
    19981 cagcctcgcg gcgagcctcg acgtgatctc cctgaacatc gccctcgtcc tcgggacagc
    20041 gggtctgccg cacatcctga tccggttcct caccgtcccc gacgcgaaga cggcccgctc
    20101 ctccatcgtg accgccacct ggatcatcgg cggcttctac ctcctcaccc cgatcctggg
    20161 ctacggcgcc accctcttcg ttggccgcga cgccatcgcc aaggccaacc cggcgggcaa
    20221 cctcgccgct ccgcagctcg ccaacgagct tggaggcccg atcttcttcg ccttcgtctc
    20281 ggcggtcgca ttcgcgacca tcgtggccgt cgtcgcggga ctcgtggtcg ccgcatcgag
    20341 cgcattcgcc cacgacttct acaccaacgt gatccgtcac ggcgaggcga ccgaacagga
    20401 gcagttccgg gccgcccgct acacggcggt cggcatctcg gtcgccgcga taatcctggc
    20461 tctcttcgcc cagagcttca acgtggcgtt cctggtggcg ctggccttcg ccgtcgccgc
    20521 gagcgcgaac gtgccggtca tcctcctgac gatcttctgg aagaagttca acaccattgg
    20581 agccgtcacc ggcatcctgg tcggcctgat ctcctctgtc gtgctcgtcc ttattagccc
    20641 gaacgtcctc accggcgtga gcccggccga gggcgtcacg ccactcatct ccgtggaccc
    20701 gctcttcccg ctcaagagcc cggccctgat ctcggtgccg ctcggcttcc tgggctgcta
    20761 cctgggcacg gtcctcggcg gacgcggcgc cgagcgcgaa cgcgaacagg gactccagac
    20821 ttcctacgac gagatccggg tccgcgccaa caccggcttc tcgaatatcg aacgggagat
    20881 ccgaacggct acaccggaaa gcgaaccccg caccacgtaa aagctccgta gcacggatag
    20941 atcccggccc tcgccgctat cggcgggggc tcttcctgtt cccggtcttc tttaattcct
    21001 cagcaactcg gcatcgagca ggaccctcac gcggccgaac tcttcttcga gcccggagag
    21061 caactccggc gcgtctgtaa ggtcgcccga gtcgcccagc cgctctagct ccgcgcagat
    21121 ggaggccatg tggttggccc ccacaccccg gcagatgccg tttagggcgt gcgccgtccg
    21181 tttgaaatcc tgggcatctc cctgatcatg gacttcacca tgcccggcga tcttccgcaa
    21241 ggcgtccaga tgggctggcg tctcggagag gaaggtgcta acgagcctct ccactatatc
    21301 gccgccgcct tctcgctgga tcaggcgcag gtccgccaga acactctggt cgagggagtc
    21361 gcctggcacc gaagcgccat tagtggccgg acgccctgct tcccgcggtt cgggcgcctg
    21421 cggactccag cgttccagta ccttatcgag ttgctcgggc cggacgggtt ttgagaggta
    21481 gtcgtccatc ccgagggcga gagccttctc ccggtcgccc tgcagggcgt gggccgtcat
    21541 ggctatgatg ggagtgtgcc gctccagtcc ctcgcgcttg cggatctccg cggtcgcctc
    21601 gtagccgtcc atctcgggca tctggatgtc catgaggatg gcagcgtatg aggtccggga
    21661 gaacgcctcc acggcttcga ttccgttgga gaccacgtcc gcgtggtatc cgcgcctctg
    21721 tagcaactcc acagcgacca tctggttcgt cagggtgtcc tccactacga ggacgcgagc
    21781 gccggactgc tccgagtccg cctcgtcaat atcctcccgg tccacagacg atgtaaccgg
    21841 agatgacggc gcaggctgga ccctctgggg ctgcttcttc aaggggagcg tgaagaaaaa
    21901 agcgctcccg acacccggct cgctcctcac accgatctca ccgcccatca gatccactag
    21961 ctgcttgcag atcgcaagcc ccagccccgt cccgccgtag cgccgggtgg ttgaagtatc
    22021 cgcctgggag aatggctgga agaggttatc ctgctgccct tcgtctatgc ctatcccggt
    22081 atccgtgacc tcgaagcgta ccttcacctc gtctccgaga tcctccaccc cctcgaccct
    22141 gagcgccacc cggccctccc cggtgaactt cacggcgttg ctcaagaggt tcgtcaagac
    22201 ctgccggatg cgaaacgggt ctcctcttag ggctcccggc acgtcgtact cgacgaagct
    22261 gaccagctcc agtcccttct cttgggcccg ctcagcgaac accgaagcgg cgtccttcac
    22321 gaccgcctga aggtcgaagt ctatctcctc tatcctgacc tctccggcct cgatcttgga
    22381 gaagtccagg atatcgtcga ggagcgcgag cagcacatcc cccgagttgc gcacggtctg
    22441 ggcgtacttg cgctgctggg gcgtgaggtc cgtatccagc aagaggccgg tcatgccgat
    22501 gacgccgttc atgggggtgc ggatctcgtg agacatgttc gccaggaact cgctcttggc
    22561 gaggttcgcc tcgtcggctt cctccttggc ccggcgaaga tcctcctccg cccgctggcg
    22621 ctccgttatg tcctgtaccg tccccgacat accaacggac tctctatcat ctccgtcgaa
    22681 tagggcttcc ccgtgagagt gaacgtaacg tatctcacca tccgacctca ctatgcgata
    22741 gtcaatgctg tagagctccc cttcaagggc ttcatctatc gcccgctcga ctcgctcccg
    22801 gtcttcggga tggatagtgt ccaggtagtg cgcctcatat gaagggacga acgactgtgg
    22861 agtgtggccg aagattcggt agtgctcatc ggaccatcgc agctcgtttg tccgccggtc
    22921 ccactcccag ctcccgaggt gagccctctc ctgcgcggcg gcgagagaag cttcgctctg
    22981 cttgagctct tcctccgctc gcttgcgctc cgttatgtcc agcatcgagc ctatgacgcg
    23041 caacggccgg ccctcgctgt cccgcaccac gtagccccgg tccacgaccg tcaggtacgt
    23101 gccgtcggcg cggcggaatc ggtattcgtc agaccatacc tccccgcctt ccctcaagac
    23161 ctcctctatg ccagcgatgg tccgctcccg gtcttcggga tgaattcgct tctcccacca
    23221 gtcacccgac gttcctcccg cgaggtcgta gccgagcatg gtttcggccg cgccgttcca
    23281 aacctgctcg ccggtcagga ggttgttgtc ccagatcgtc tcgctggttg cccggctgac
    23341 gagccggtag cgttcctcgc ttacccgcag cgcctcctcc gcggccttgc gcgccgtgac
    23401 gtcgtggact atcgagtaga gtacggtccg cccgcctatc tcgatggggc tggaatgcac
    23461 ctcgacatcc cgtatctcgc ccgtcgagag cctgtgctgg aagatgaaat acccgcgctg
    23521 gtccgtcttc gcgcgcgaca tctcctcgtg tacctgatcc ggaggcagcg tgtttaggtc
    23581 ggctatgttc ttcgccttca ggtcttcgag tccgtaaccg taatactcgc ccgccgccag
    23641 gttggcgtcc aggatgtctc ccgattcggg gtctaccagg agcttgaccg cccggttctt
    23701 ctcgaagacc tgccggtaac gctcctcgct ctctctgagt tcctgttcta tctgcttgct
    23761 ccgggttatg tcgcggacgg cggccacgcg gacggggcgt ccctggtact cggacatctt
    23821 gccccggatc tcggccagaa agcacgtgcc gtccttgcgc aatccgatca cctcataagg
    23881 ctcctcggaa ccggcgaaga tgctgcgccg caccagctcg cgggactcgg ccgtcacgaa
    23941 ctcgaccgcc tccataccca ccacctcatc ggtgtcatag ccgaacatac tggcgaaagc
    24001 ccggttggtc tcgaggacct tgccttccgc ggttatgacg atcccctcga agctcgcctc
    24061 tgcgagaccc ttgaagcggc tctcgctatc ccggagagac cgcccgatac gcttctgccg
    24121 gttgataaag aactgcgcat atccgctaaa ggcgacgata agcgccacga ccgtgaagcg
    24181 catccatagc tcgttaggat cgcgcggcca caaccgctcc gtgaaggtct cgtcgtgcac
    24241 cagagtgtcc gtggctgact cgataatcca gtagagcgcc gcgacaatga gacccagcca
    24301 caacagcctg tcctcgacgc tcctctttag gagagcaaac ctcttgtctc tcatcccata
    24361 ctcctcaact caacccatcc tccccgcgct gagttgttaa tagatcttcg atccaacgct
    24421 gcccaacgtt tggctggaag tatatttcat tactagactt gtcggacagt acgcaaggaa
    24481 actttagcgg taatagatct tctttgttac gtgagcgttt cccgctgcta cacatgttct
    24541 tccgctacca tataagcatg gagatagaga tccgtcacgc cgaaccggat gactacgaag
    24601 ctctccaccg ggtctttaga gggccgaagg cgatagcggg cacgttacag ttgccgtttc
    24661 ccccggccga aacgtggcgg aagcgactct ccgaaccagg tgaaggaagc ttttcactcg
    24721 tcgcctgcgt ggatggcgag ttggttggcg agctgtcgtt gtggactagt cccgccaact
    24781 ggcgcattcg ccacgcgggc ggcttcggga tggccgtacg cgacgactgg cacggcaagg
    24841 gcatcgggac ggcgctgctg gaatcggcgc tagagcttgc ggacaactgg ctcgatctga
    24901 agcgagtcga actacacgtc tacacggaca actccgctgc tatagcgtta tacgagaagt
    24961 tcgggttcga gatcgagggc actcaccggc gcttcgcctt ccggggcgga gagtacgtgg
    25021 acgcttattc gatggcccga ctgcgaggat aactctccca ctcatccagg gaaggggagc
    25081 agttagaatt caggcatggc gagagcggtc gagaacggcg gcgtctgcgg gctgtgtgga
    25141 aggatggtgg agcggcgcac ccggcaccac ctgatcccgc gcagccgcca cgggaacaag
    25201 cgggtgcgcc gctccttcgc ccgcgaggaa ctcaaccgta ccgtggcgct atgcccggcg
    25261 tgccaccgtc agatccaccg caccctcacc gagaaggacc tggagcggga gtacaacacc
    25321 gtggaagcgc tcctctccca cccggatatc ctgcgcttcg tcaggtggat agagagaaag
    25381 ccccacggcg tgataggccg cggggctcga cgatagtccg gctgagatcc taaatctagg
    25441 ccaggctggt attcaactgg atgtccacgt tgccgcggag ggcgtttgag acgggacacg
    25501 tctgctcggc ctgctcggcg gcgctgcgaa aaccatcttc atcgaggccc ggcacctttc
    25561 cgcgaacgtc cagggtgacg gtcgtgatct tgaggctgcc catatccagc acgcagtttg
    25621 cgctgacgtc cagctcctcg gggtcgttgc cctgctcggc cagggtgttg gagagcgcca
    25681 tcgcgtagca gctcgcatgg gcggcggcga tgagctcttc cggagaggtg ttgccgttag
    25741 ggttctcggt gcgggtggcg aagctgacct cggcgccggt ccacgcgccg ctgctcgcca
    25801 ggtcgaaggt cccggagccc ttcatcaggt tgcccttcca caccacgtcc gcgcgtcgtt
    25861 ccgctgtagc cacaagctcc tcctacttaa tcggtttacg cgaatagcag gtctggattc
    25921 tacccgatcc agcagtctcg aacctgagtt gacgcagctc tatccctgtt ccagactggc
    25981 gctgacgtct atccggttgt tgccctttat agcgtccggg acgggacaga tgcggtgagc
    26041 ctctccagcg gcatgaagtc ctcaagcgtc tcggaagcaa gaagcccgga tcgtccgcgc
    26101 cggtatgctc cagcggggag ccggccgctc tctcagtcgc cggaagcttc ggccgcctcc
    26161 tcgaccttgc tccggtagac gggctcgtcc gtcccggcaa cctgtatcgc cccggtacgg
    26221 gtgatggcgc cattaaacag gaaggccgtc tggcgcaggg aggactcaag gtcgaacggg
    26281 tcgagcgcgg aggtcgcgtc tttcgggatg atgaccttgt accatcgcag cgcggcgctc
    26341 gcggcggtgt agtggacgca gatgctcgct acagtgccgc agatgatcag ggtatccacg
    26401 tcccagatcc gcaggaagtg gtccaggtgg gtgccataga aggcgtcgta gcggaccttc
    26461 ctgatgacca gctcgtcgtc gcgcggcgcc aggtcctcga cgatctccca cccccacgag
    26521 tcctcgcggc agtgctccgg ccagatctcc cactccgggt cgccctcggt gtgcgtgtcc
    26581 tggctgtaga cgaccctcat ccccgagtcg cgggcgagcg acaggaggcg ttggatctcc
    26641 ggtatggtcc cctccgcgtc cgggacgacg agcgtgccgc cctccttgac gaagtcgttc
    26701 tgcatgtcca cgacgatgag cgccgtcctc gcgggatcca cccgcacctc gccatgaact
    26761 tcgtactccg ggacctccac cgtgcgggcc ataagctcct ccttctgatc tcagatctcg
    26821 gagcctagtt taccgtccct cgcggcgata aatggatggt gccgccgggc tccgagcgta
    26881 taactcgatg gccgccgagt agacgcgcgt cgctacttgc cccgccgcag gaccggcagg
    26941 acttctccaa taactagagc ggagaggatc agtaccgctc caaagatctg taccgttacc
    27001 agcctgtctc ccgccagcca gtaaccgaag atggcggcga agacgggttc catcgtcagg
    27061 atgatcgcgg tccgcgccgc cggtatcttc tgttgcaccg tcgtctgcac caggaatgcc
    27121 cccgccgacg ccagaaaccc ggtcacgagc agcgcaaccc acacccccgg cggcgggaac
    27181 gagacgttgt cgaagaacgg ccacacgatg ataaagagga ccgccatcgc gagtatctgg
    27241 gcgagggtga gcgcgcccgc gtcgtgcccg gcagcgtagc gagagagcag ggcgatgtgc
    27301 aggccgaggg ccgccgcgca caggagtgtc aggaagtcgc cggggttcac tccctccggt
    27361 cctcccccgg cgagcaggac catcccgatc aggctcagaa agaccgccac gagtacctgc
    27421 cgcgaggcgc tggccccgaa gaggagccta tccgccagcg gcgcgaagac cacgaaaagc
    27481 ccggtgatga gcccggagtt ggtcggcgtc gtgtacagca gccccgtggt ctgaaacagg
    27541 tagcccgccg cgagcactat tcctataccg cctccgacaa gcagcgagcg ccgcgtgagc
    27601 ctcgccgcgt aaataggcgc gagtgtggct ccggccagga cgaagcggac agccaggaac
    27661 ggcagcacgc cgtagagcgc gatggcgtcc tggaccacca caaacgtcca gccccacacg
    27721 gcggtaacgg cgacgaggag tagagtgtaa acgagcctca tgcccattcc tcagcggatt
    27781 tctagcttcc atgacccgga gaagataaca cagaaatgag ccagataagc agtcaatttg
    27841 agcgacaagc attcagatag ctagtcaatt ttcggcaaca tgctagagct aaaattgcgg
    27901 cgtgttgcag accagcttct atgccagctt cttgatgccg ccaccgtgcc gcgatgagtt
    27961 cgcggtccat cgccgcaccg tatctctatc ctggtcctct tgttgagacg tgtcgtcctt
    28021 caacagttct tggtcctctg gcgtatggta agtctcatcg gtcatggaag atagacggac
    28081 gttgagccgt gctagttctc tgtgcctggc ctcttggaat ccctctgtcg ttgggacatc
    28141 ctgtgatggg atacgagtgg ctgcatcttc catatggcag ccatcttagc tacccctgtt
    28201 cgcgtcgtct acgcggccca aaatatgtag acgaaatatg tagatcgctt tgcatctatt
    28261 tatgtagtta tcaacgaaaa ccgatgttgc tccatttccg cagactgttt gcctttaagc
    28321 tacagcgcct cctccggtcc ttcttggaac attttgagcc ggcctcgcag gttgctcctg
    28381 acctcgggcc actcgtcgtc gatgatgctg tagtaggccg agtcgcgtag ttgaccgccg
    28441 cgcacgagca tgtgcttgcg gaagatcccc tcgaaccggg ccgggagcgc ggcgagggcg
    28501 ccgcgggaac cctcgttgcg cgcgtcggtc ttgaactcta cccgcataca gccgagacgc
    28561 tcgaaggcgt gctccagcat gaggagcttc gcctcgacgt tcgcgccggt tttccaacgc
    28621 gacggggcga gccaggtcca accgatctcc aacccccggt gctcgggccg cagggccagg
    28681 taacgggtgc tgcctatcgg cactccggtg tcggcgtcga gggtggcgaa ggcggcttcg
    28741 gttccggcct ccgagttggc gagagcagca tcgaaccacg cgcggaacgt ctcgctgctc
    28801 tctatagcgt tgtatggcat ccagcgccac acccgcgggt ccccggcggc ctcgaagaga
    28861 ccctgctcgt gccgggacgc cagcggctcc agcctcacgg tctccccttc cagaacgctc
    28921 gccaggttct cccacataac cgccactcct tagctgtccg atggttccca cggcccaacc
    28981 tatacgcgag ctggcggtta cgcaaggctg acggaggttc ccctgctctc cgaacgcaag
    29041 tcctatcagt gtgagaagct agtgtatgcc gctggcgcgg ggggaacatt accggaagat
    29101 gagaggagag catgtccgga tacagaaccg ccgacgggtt gatagcctac cggagatacg
    29161 gcgaggggac tcaggagacg gtgatcctgc tccacgcctt cccgctaaac agccggatgt
    29221 ggacgccgca ggctgaggcg ctcgcaggca gcagccgcgt tatagcgccg gactatccgg
    29281 gtttcggacg ctcgccgagc tcccccgccc agccggatgt ctgctactac gcgaagtgcg
    29341 tgcacaggct gctggaccgc ctgggcctag aaaaggtcgt cctgggcggc atctcgatgg
    29401 gcggatatgt cgccttcgag tgcctgcgtc agttcccagg tagggtatcc gccctggtcc
    29461 tcgccaacac ccgccccgac ccggactccg aggagatccg ggagagccgc cgggagatgg
    29521 cccgccgcgt cgccgccgaa ggggtggagg tcctggtcga gcttcagatg cagcgtctcc
    29581 tcgcgcagag caccctgcaa aacgacgaga ccacggtcga gctggtccgg tccatgatcc
    29641 ttgagagcaa cccgaacggc gtcgtcgcgg ccctctcggc catgcgcgag cgcccggact
    29701 ccaccccgct cctcccagag ataagcgtcc cgaccctcgt catcggtggc gcggaagacg
    29761 agatctccac ccccgaagtc atgggcgaga tggccgcgaa gataccgaac tcccgccaca
    29821 tcgtcctgga aggcgccgga cacctctcga acctggaagc tccgctggag ttcaacgctg
    29881 cgctgaaggt cttcctgaag gataattagc ccgtggacgg cggcaggata ctcgtggtcg
    29941 gggcgacggg cagggttggc ggcgcggcgg tccgctacct gctggaggcg ggcttccagg
    30001 tgcgcgccct ggtacgcagc gcgcagaaag gcacggtccc gcgggcgcag ggcgccgagg
    30061 tagtagtcgg cgacgttacg cagcccgcga cgcttgcgcc cgcgctcgaa ggttgcagcg
    30121 gcgtcttctc cgcgctctcc gccgcgacgg accgggcggc ggtcgaggtc gagtaccggg
    30181 ggaacgttaa cctgatctcc gcggcgcgcg agaacggcgt gcggcgcttc gtctacagct
    30241 cggcgttcct cgtggaccat ccgctcgccc aggaagtcgg caccttccgc gagaagctgc
    30301 gcttcgagca gcacctcctc gaagcgcagg acgtctcctc taccatcctg cgcccggtct
    30361 cgttcatgga gacgctgcta ctgtcgatga gcgggccttt cgcgttcgtg atcggccgcc
    30421 agaaccggcg ggccggatgg atctccgcct ccgacatagg ccatgccgcc gcccgcgcct
    30481 ttgagaggga cattaccggc cgccacgagc tggccggccc ggacatcgct accttcgacg
    30541 aggcgtaccg tcacctgtcg cgggcgcggg gcaagaggat aatagtgctc cacgcgcccc
    30601 tcgccgcggt gcgcgcggtg ggccaggccg tagctccggt cgaggagctc gccggcatat
    30661 tcaccctctt cgacgccgcg ggatacgtct cggagtccac ggcgctgcgg gatactttcg
    30721 gcgtggatgg gatttccatc gaggagtggg ctggacgggt ttccagttcc ggccaagtcg
    30781 ggtagaatcc ctcaccgtga ctcgactagt acatgtatca gacctgcact tcggtcgtcc
    30841 cgctgtcgcc gaacgcctgg aaggtttgag agaacttgtc cgcaagctcg agccggacgc
    30901 gctggcggtc tccggcgacc tgacgcagcg ctgctccaac agggagttca ggatggcgcg
    30961 ccaatacctc gactcaatcg gcgagatagc cccgtatctg gtgatccccg gcaaccacga
    31021 catccgctgg ctcggggccg tggcccgcaa cctgggcttc gccgggctgt tccagaagca
    31081 ggcccacaat ttcaagtact ctcgctacac caggcacatc tccgaggagc tgagcccgtc
    31141 cctggaagtt ccgggagccg ttatcgcggc gctcaacacc gcgcacggga tcacccgcgg
    31201 ctcgctgacc aacaggttca aggacctcgg cgtgatcggc tacgtcagag gcgaggacat
    31261 ccaactcgtg agagaggctt tcgagaacgc cgacccggac gcggcccgga tcgtcatgat
    31321 ccaccacaac cccatcgagg gcgcctcctc cggccggcac ggcctctcca acacggaaca
    31381 ggccctccag gccttcgccg acctcggcgc ggagctgatc ctctgcggtc acgaccatca
    31441 ggaggccgtc cacaccgtcg agcgcggcgt ccgcggcatc gtcatctcta ccgccggcac
    31501 catctccaac cgcctgcgtc cgggacgcgc gtccagcttc aacctcgtgg agacggacga
    31561 gaacagcctc cgtataaccc cctactcctg gcaaaacccc gagggcttca ccccctccga
    31621 ggagagctcc ttcccccggc gcgccaccac gcgctcggcc tgatctcccc gatcacttcc
    31681 gcaccccatc ccagcgggta taatctgccg cgatgtgctt acccggactc acccggaagg
    31741 agaggtacat agcatgtgga gcagactgac ccgtggcggc gacggactac cccgcgagaa
    31801 agttgacgcc atcgtccgcc tgatagacca gtacctcgcc gaagaggcca ccctccgcaa
    31861 cctccagtcc gataaacaga ccatccaccc ccgcgacctc ccccccgaca aacgcgaagc
    31921 cctcatagcc gagatcttcg acatcctcaa agacaaccgc gacaaataag acaggcaacc
    31981 aaccggttca ccgcctcatc cgaatacaac gctccctctt gacataggat aggggggtat
    32041 agtaaatata ccctgacgat gctgattaga ggaccggagg gcggcgatgg ctgagaccag
    32101 ggagcggcgg gccagtatct cgattccggt aacgggcatg tcgtgcgcgg cctgcgcgaa
    32161 ccgggtggag agggctctct cgaagacggg cggtgtctcg aacgcgagcg tgaacttcgc
    32221 caccgagaag gccagcgttg agtacgaccc ggacgccgtg cggccggagg ccctgatctc
    32281 ggccgtcgaa gacgccggtt acggggccga ggtgcgcgag acgagcttcg gggtgacggg
    32341 catgacctgc gcgagttgcg tcgggcgggt cgagaaggcc ataagaaagg tccccggcgt
    32401 gctggacgtg agcgtgaacc tcgcgaccga gaaggccagc gtggagtacc tcgcgggcga
    32461 ggcggagatg cgggacttcc agaaggccgt cgagggcgcg ggctacggcg tggtccgcga
    32521 ggaggaagag gcgtccgcgg aggacgctca cgagagggag tacgcgaagc tgcggggacg
    32581 tttcctggtc gcggcggcgc ttacggcgct cgtactgatc ggcagcctgc cgatgatgtt
    32641 cagcttcgag gcgccggtcc cgatgcggtg gctcaacctc gggctgctcg cgctggcgac
    32701 gccggtccag ttctgggccg ggtggcggtt ctaccggggc gcgtggggcg cgctcaggca
    32761 cggccaggcc aacatgaaca cgctcgtcgc ggtcgggacc agcgcggcct acctctacag
    32821 cgcggtggcg acgctcgcgc cggggctgtt cgccgggagg gccgatgtct acttcgacac
    32881 atcctcgctc atcgtcacgc tcatcctgct gggcaggttg ctggaggcgc gggcgaaggg
    32941 acgaaccaac gaggccataa agaagctcgc cgggctccag gccaggaccg ctcgcgtggt
    33001 gcgggacggc gaggaagtag acatcccggt cgaggaagta gaggtaggag ataccgtggt
    33061 cgtgcgtccg ggagagaagg tgccggtcga tgggcgcgtc atctccggcg agtcggcggt
    33121 ggacgaggcc atgatcacgg gcgagtccat ccctgtgacc aagcgtaagg gcgacgaggt
    33181 gatcggagcg accatcaaca agaccgggac gttccgcttt acggcgacga aggtcggccg
    33241 ggacacggcg ctcgcccaga tcatgcgcat ggtcgaggag gcgcagggct cgaaggcccc
    33301 gatacagcgc ctcgcggata ggatcagcgg cgtcttcgtc ccgatagtga tggtcctggc
    33361 gacggtcacc ttcatcgtct ggtgggcctt cggcccggaa cccgccttca ccctggcgct
    33421 gctcaacttc gtcgcggtcc tcataatcgc ctgtccttgc gcgatgggtc tggcgacgcc
    33481 gacctccatc atggtcggca ccggcagggg cgcggagcga ggcatcctgg tgaagggcgg
    33541 ggaggcgctg gagcaggcgc accggctcac gaccgtcgtg ctggacaaga ccgggaccct
    33601 cacccggggc gagcctcgac tcacggatgt gatcgctact aacggactct ctgaagagga
    33661 gcttctcagg ctggcggcgt ccgcagagcg cggcagcgag catccgctgg gcgaggcaat
    33721 cgtccggggc gcgaaggatc gcggcataga gttggccaat cccgaggact tcgacgcccc
    33781 caccggacgc ggcgtgcggg cggcagtgga gggcaggagc gtactcgtcg gcagccgcaa
    33841 gctgctggac gaatacggcg tctccgagga tgggctcata cctgaggcaa aggaaatgtc
    33901 ggcggatggt aagaccccgg tcttcgtcgc ggtggatggc gaacccgtgg ggctgatagc
    33961 ggtggccgac acggtgaggg aggagtcgaa ggaggccgtc gaggcgttgc ggaagatggg
    34021 actgaaggta gccatgatca cgggcgacaa ccagcgcacc gcggacgcca tagcgcgcga
    34081 gctgggcata gaccgcgtgc tcgccgaggt gctcccggac cagaagtccg gtgaggtgaa
    34141 gaagctacag gccgagggcg agatagtagc gatggtggga gacggcatca acgacgcgcc
    34201 ggcgctcgcc caggccgacg tgggtatcgc catcggcacc ggcacggacg tggcgatgga
    34261 ggccgccgac ctgacgctca tctcgggcga cgtgcgcggc gtcgcgcggg cgataaagct
    34321 ctcgaaggcg accatccgca acatccggca gaaccttttc tgggccttcg cctacaacgt
    34381 gctgctgatc ccggtggcgg cgggcgtcct gtacccgttc ttcgcgggct ccgcctggac
    34441 ggtgccggag gcgctgcgcc cgttcctcgg cgagtacggc ttcctgaacc cggtgctcgc
    34501 ggcggcggcg atggcgctct cgtccgtaac agtagtgagt aacgccctga ggctgcgccg
    34561 ggtaaagata ggttagggag gatagtgatg caggatatcg agaacgcatc cggagcgcgg
    34621 cacggctaca tcaaggcgca cgacaaggag aaacttcaga accgcctgcg ccggatagag
    34681 gggcaggtgc gcgggttgca gcggatggtg gacgaggagg tctactgcat tgacatcctc
    34741 acccaggtcg cgagcgtcgt ctccgctctt gagaaagcc
//
LOCUS       DASSMB010000059        18909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1153340, whole genome shotgun sequence.
ACCESSION   DASSMB010000059 DASSMB010000000
VERSION     DASSMB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 18909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18909
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153340"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1452
                     /locus_tag="VFJ72_04095"
     CDS             169..1452
                     /locus_tag="VFJ72_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478681.1"
                     /translation="MDNRRATEQAERPGTLPPSTLRGRWLTFAQVAWVAVALITLGLD
                     AISIPYYYARDRAVCAACAGDSGRITPDRLRALHDMGLSAGFYAGFDTAVEIIAVLVF
                     AVVAAVIFLHRSHDRMALFGSFTLLVFGGAAFSSDLLKGLVAAHPVFRFPTELLDYIG
                     QVCFITLLYVFPDGRFAPRWTRWLAVVSALLWVPTIFFSASSLDLLSGPYIVGLVGAA
                     MVAQVYRYRKVSSPAQRQQTKWVVFGVVVAITGFSGMITFSNLVPSVQRAGPLVQMGA
                     TAFINGFLLLIPLSIGVAMVRSRLYDIDVVINRTLVYAALTISLASVYLGVVVALQYI
                     FRALAGETSQIAVVASTLVIAALFNPLRRRVQALVDRRFYRKKYDAAKTLEAFNVRLR
                     DEMDLGQLSRDFISVVEDTMQPEHVSLWLRPPENK"
     gene            complement(1489..4389)
                     /locus_tag="VFJ72_04100"
     CDS             complement(1489..4389)
                     /locus_tag="VFJ72_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7478682.1"
                     /translation="MPDTRLFLLGSPRIEHAGAVIEVDTRKAVALLAYLSIEEGRHGR
                     DALAGLLWPEYSQSKSRAALRRTLSALSGLRSAGPLEVDRESAALNRDGTWIDAVRFR
                     ELLERCRTHGHPETEVCAACLPILSEAAGLYHGDFLAGFGLRDSFDFDDWQYFQAESL
                     RRELGGALERLARGYGARNEWEQAIAYARRRLALDPLSESAHRALMQAYALSGQRADA
                     LRQYRECVRTLEKELGVPPLAETTGLYRTIEQGGTPPPFSIHTVPEADTLQPESASEV
                     RFSGSPMVGRAREWEDLRKAYGPEARGYVVVLEGEAGIGKTRLAEEFVAHVRESGAVA
                     LAARCYAGETNLAYGPFAEALESALGQTDVASRLEEIPDYLLSEARRLLPGLAGLFPD
                     LPDAPPLDAPGAQSRFFEGVSRVLLAVCGGEPSGILFLDDLQWADDASLDLLSYLVRR
                     LDAKPLRVLVAWREEEVPEGHRLRALVREAQRAGHARILELGRLEAPSVRELVRSVAG
                     EDEELERRLYDETEGLPLFLTEYLAAAVRGELGPESGAWSLPGGVRDLLEGRLQSLNE
                     TGKQLLAAAAAIGRSFDFDTAREASGRSEEETIAAIEELGSRFLIREVPESAKEGYPV
                     YDFSHEKLREIVYEKTSLARKRLLHQRIATALADRADRRREGGSLAGQISYHYRMAGQ
                     DAEAAEYAKLAGEHARALYANGEALSHYQTALALGYPDAAALHAAIGDLRTLTGEYEQ
                     ALASYETAAALSAGEDLAGIERKLGDVHGRRGEWELAESHYAAALEALGEDGSPERAR
                     LYADRSLLAHRQGQDEAASEFARRALDLAEEADDERALVQAHNALGILASSRGDFGEA
                     RRRLERSLALAESLNDPEARIAALNNLALALGRGEEVEEAMDLAGTALALCVSLGDRH
                     REAALRNNLADLLHTAGRQEESMSHLKEAVAIFAEIGEEGALRPEIWKLVEW"
     gene            4669..5928
                     /locus_tag="VFJ72_04105"
     CDS             4669..5928
                     /locus_tag="VFJ72_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478683.1"
                     /translation="MMKIDGGLRMWAAVLVALAAGLVLVAAQSARAGAFPGVNGKIAF
                     VSNRDGNDEIYTMNPDGTGLRQLTRTLYPSGNESPAVSPDGSRIVFERNKDVWIMNAD
                     GSGLRQLTTDGAFDHDPTFSPSGAKIAFLTRRGTDAPISRDNIWTMNTDGANQTRVTS
                     NGAIEPTWSPNSDVIAFVRAGALWVTNLTTNTETQYTFTNTGNPDTSVGGVAFPTWSP
                     NGSLIEFTGIGGALHCSCSGYFSVSAAGGAPTTIGPDDPSDKFLAFSPDGARIALQST
                     NRERNEEIYTMNPDGSGLTNISHDLSFTDNQPDWGPQVTQQSTSLSLSANPATITSGH
                     SSTLSGRLVTASGKVYAGQRVTIQQKPKGASRFSNLKAVSTAFDGRFKTTVKPKKTTY
                     YRAVFGGNGVLGLGASTSPARLVKVSG"
     gene            6576..7850
                     /locus_tag="VFJ72_04110"
     CDS             6576..7850
                     /locus_tag="VFJ72_04110"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET7478684.1"
                     /translation="MSFGNAILDAIEVKKFDGTLSEAVIREVVEGYTSGAVPDYQMSA
                     LLMAVFIRGMDYGETLALTRAMAESGDTYSFPDCVDKHSTGGVGDKISLTSLPIVAAC
                     GAPVAKLSGRGLGTTGGTIDKLESIPGFSVDLSRAQFERQVAEVGLAIIEAGELAPAD
                     KEIYALRDTTGTVDSLPLIGSSIVSKKAATGAGHLLYDVKCGSGAFMKTLEDARELAG
                     LLVRLSGSLGIRASALITDMDNPLGSAIGNALEVRESVRFLKGEHVPEDLAHEARLVA
                     VRLLELKGIPDPERAVEEALSSGAAYEKLREFVRAQGGDADALDDLPISDEVREVAAP
                     RAGYVVRFGAMGVGRAALMLGAGREKKGDSIDFGAGVEVLVGIGDKVEEGQPVARLYG
                     ARRTERAERLVLEALELSGEPVEPPPAILDSL"
     gene            7931..8647
                     /locus_tag="VFJ72_04115"
     CDS             7931..8647
                     /locus_tag="VFJ72_04115"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HET7478685.1"
                     /translation="MSPVHVRAEPGDFAESVLLPGDPRRAKYIAENFFEDAKLVTEER
                     GMLGYTGTYKGEPVSVQTTGMGCPSASIVTEELIQLGCKNLLRVGTCGGYHEDLRLGD
                     LIVATAATPQDGTVSSITQGVPYAPAAHFDLVHAAHHAAESAGRRTFMGPIVSSDLFY
                     DPEDDPATLWSNLGVLAVEMEAAAIFTIAAMRGVRAGCLLTVSDTIGREAVVRIEDSA
                     LRDAVDNMMALALDTLNALN"
     gene            8735..10051
                     /locus_tag="VFJ72_04120"
     CDS             8735..10051
                     /locus_tag="VFJ72_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HET7478686.1"
                     /translation="MTILNESGVADTLSPLHALSGLAEKVPGAVIAVIGTRAEAFLAQ
                     SLDAAIPDRSPAIRRGPRVVFVVLDPAHPPEGGRIASRLIAEVAGISGTRMLLLVAGR
                     SAELLGVDANFEARLASRRLDIPAAAVALDPDGNSPGSLSTDLEDRALAALVSLCPTG
                     TSENLELPATAPKRGGLLGNFLGRGREETRSGHRKAVVLLGSSGEELARELERAGVEV
                     AGSVPGPDATVLPDIGEGTIVALTDPHLTAASRAAEERGAQIVRTLTPIGVDGTARFI
                     QDAAAAAGTVANELGRAREAWEEMEHLRNRVRGKRVFFAGDTGYEIPLARFLADAGAV
                     VLEVGAPRLDRRGLGAEIQAMGADVDIVQYPDWRGQIQRADETRPDVVITSPGLYAPL
                     VARGHLCRCARDFLTAGPHGYEGARRILETLIRTFERAELLDSVNL"
     gene            10086..11522
                     /locus_tag="VFJ72_04125"
     CDS             10086..11522
                     /locus_tag="VFJ72_04125"
                     /inference="COORDINATES: protein motif:HMM:NF012375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HET7478687.1"
                     /translation="MRGVYEGPGSHGILRVAASMTNVHAVLRALPHEGYFHALYGAWS
                     RSGEAPPVTLSPVWGDSAKQEASMAPGHLRRDLSQVLRRHPGTESVILARSEAALLSG
                     EEIPTDALPENPETGLTPGLVACDWESPLVREIEAADLALEDLVRAHAKGRERSPDPT
                     VNIFGPPIFSPGAAAEYDEAERLLDLIGVQVNARVPLDAGVRDLARLPRAWANLVLYR
                     ETCESATLYLQDEFGTQRVTPPMIGSAGTGAALRSVGELCGLEPQRVQRVVWAELSRT
                     SRLPWYARLATPETFEDRRVAIFGDFTYTLGLGYALAREVGLEVAPCGTYLTHLERDF
                     LFHAHTFTEDAFVTDDPDEVADRIEASRPDLLIGTHLEGAVAEALNIPFLPLCPPVTT
                     HPFVQRPLMGYAGSSVLADALERSPRWTEEKPRAIPAGLPWTDDALDKLEEIPAFLRG
                     RARRMAEERARWLDAPEVTPEILAEARR"
     gene            11554..11835
                     /locus_tag="VFJ72_04130"
     CDS             11554..11835
                     /locus_tag="VFJ72_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478688.1"
                     /translation="MGIRDFIEYLREHQELLRNGAELPEAEREETLREAEVMVGMAEA
                     AVKQLESVIKNRRVNLPEEHAVRFAQDIFRHLIAEEEKVKEKLREQVSP"
     gene            12034..13146
                     /locus_tag="VFJ72_04135"
     CDS             12034..13146
                     /locus_tag="VFJ72_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7478689.1"
                     /translation="MLKSRIYFLPLLAALAAVLFVAGCSLQNGQGNKAENVAHKPAPA
                     SPKIPETEPVARVASQVEPSVVQVNVRSIQMTPFGNQAQEGLGSGVIYRKDGYIITNN
                     HVVQGASQVNVAFADGSTERGQVVGTDPTTDIAVVKVNRNNLPAAKFRKSENLVPGQL
                     AVAIGSPSGFQSTVTAGIISGLNREIPASLTGGTDTSLVDLIQTDAAISPGNSGGALV
                     DRAGEIIGINVAYLPQTQTGQLVEGIGFAIPSDTAVSVADQLIANGKAVHPYLGIQLA
                     DLTPEAAKQFNLKVDSGVVVRNVVSGSAASNAGVRSGDIITAIDNRKIADSGDLLGDL
                     RDYKPGDQVKITIVRNGNKKTLNVTLDQAPQQQQQQ"
     gene            13257..13655
                     /locus_tag="VFJ72_04140"
     CDS             13257..13655
                     /locus_tag="VFJ72_04140"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7478690.1"
                     /translation="MAHFKTTSADVRLLEPRDVLDGGEFAGIDERVAGLMGAMAGPTR
                     IRALFALLDAGELPAGEVAKAVRMSASATSHQLRVLRDLGLVNRRREGRKCFYALADE
                     HLGVLLKEALYHVDHTRLAEQAAGGRGASR"
     gene            13652..14641
                     /locus_tag="VFJ72_04145"
     CDS             13652..14641
                     /locus_tag="VFJ72_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7478691.1"
                     /translation="MKRSRSHPAGHQHETGRWAKVWHTLSHLIPFGHSHESSESVDDA
                     LESSKAGIRALKISLVALMLTALMQAGVVVLSGSVALLADTIHNFSDALTALPLWLAF
                     ALSRRATNRRYTYGYGRAEDIAGLAIVGFIFFSACVAGYESVIRLISGSEVSNLGWVA
                     VAAGIGFVGNEFVAQFRIGVGRRIGSAALVADGQHARVDGFTSLAVLLGAAGVALGYP
                     VLDPLIGLLITVAILFIVKDSARSVWRRMMDAVEPEIVSGIEEAAASTEGVEKVESVR
                     ARWVGHRIHSEVRIRVDGGLSVDEGEEISGRVRDAVRRATPRLDRVAVEIGAL"
     gene            14670..15647
                     /locus_tag="VFJ72_04150"
     CDS             14670..15647
                     /locus_tag="VFJ72_04150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7478692.1"
                     /translation="MAHDHTHHNHGDHGGDGHSHGVSEDADRRYLAIALALLVGFMLV
                     EVVVGIVASSLALISDAGHMLTDAGAIGLALVTMRLAQRPARGVMTYGLKRAEILSAQ
                     VNGITLLLLSAWFVYEAIRRLVHPPEVEGGLVLGIALLGIVVNLLATWSISRANRQSL
                     NVEGAFQHILTDLFAFIATAVAGAVIYFTGGYNRVDAIAALVVAALMFKAGYSLVRDS
                     GRIFLEAAPKGFDPDEIGSVLASRPGVAEVHDLHVWTITSGFPALSAHVLVGRDEDCH
                     ARRRDLEEILAEEFDISHTTLQMDHVGDHHTGNTNLQFFPDIQRRGEGK"
     gene            15766..16365
                     /locus_tag="VFJ72_04155"
     CDS             15766..16365
                     /locus_tag="VFJ72_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET7478693.1"
                     /translation="MGKQRPGSRTTPRRLDNAGRERPWGLIIVGGILALVLIAFVTVV
                     VLDSREQAARTPPSEVKTYKVERGHTESNVKYAQSPPVGGQHNPVWQNCGFYSQPVRN
                     ENAVHSLEHGAVWITYQPDLPKDQVNTIKDLTESQDYILASPYPGLDSPVVASAWGKQ
                     IKLQSANDSGLKQFISAYRKGPQTPEPGASCTNGTGQPG"
     gene            16372..17022
                     /locus_tag="VFJ72_04160"
     CDS             16372..17022
                     /locus_tag="VFJ72_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET7478694.1"
                     /translation="MKSASRRSARRPDPRTLVALIAVLLAATMLVMYIAGRPPGDGSP
                     EAGFARDMSVHHGQAVEMAGIVQLRTKDPKIRSLATDIVLTQQSQIGTMQGWLDVWGL
                     SPTGTEQQMAWMGHPMSGRMPGMASPKEINRLSTLPPEKTDVLFLQLMIPHHKAAIPM
                     AQAVLDRTNQPEVQRLADSIIASQKSEIEAMQAMLKDKNASPAKEDPKMDMKSMDH"
     gene            17063..17419
                     /locus_tag="VFJ72_04165"
     CDS             17063..17419
                     /locus_tag="VFJ72_04165"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7478695.1"
                     /translation="MNDERCDLLCIDAPLAEGIRTRLPGVEAARAAAERAGALSDPTR
                     LLLAVALAEGGELCVCDLAWISARAQNLVSHHLRLLRSHELVTSRRDGKLVMYSLTNT
                     GRSLLTAVMGEVRVGQ"
     gene            17455..18108
                     /locus_tag="VFJ72_04170"
     CDS             17455..18108
                     /locus_tag="VFJ72_04170"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET7478696.1"
                     /translation="MRSAEREGLVRRAKLLAWLGVGWHGVEAAVAIGAGAVAGSIALV
                     GFGADSLVESVAGFVLLWRFAASRASSESAERRAQKLIAVSFYVIAAYVGFEAVRTLL
                     GAEQPDASWVGIGLAAFTLLTMPPLAIAKARVGEKLGSSATKSEGRQNMLCAYLSAAL
                     LVGLGANALFGLWWADPVTAILISVVAIKEGRDSWRGESCCTAPVIAGEDCEDDCCR"
     gene            18071..18454
                     /locus_tag="VFJ72_04175"
     CDS             18071..18454
                     /locus_tag="VFJ72_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478697.1"
                     /translation="MPERTAKTTAAGKRGTSIRDRDLDALPIACNLTGREASQRKEST
                     IEVLRRAEQAREIEDGYELLFPGDDEMVRGITEVVIAERSCCPFLAFETLFEPDGGPV
                     LLRVSGPPGAKDFIRDELAMTTIEE"
     gene            18451..18834
                     /locus_tag="VFJ72_04180"
     CDS             18451..18834
                     /locus_tag="VFJ72_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HET7478698.1"
                     /translation="MTFILDRLKRWARKLENEVYALYLAYKDPRVPWYARALAAVVVG
                     YAFSPIDLIPDPIPVLGYLDDVILVPLGIALTIRLIPDEVLAECREKAKTMQDRPVNR
                     VAAAIVVAIWVLLVIAAFMLVRRYF"
BASE COUNT         3376 a         6230 c         6169 g         3134 t
ORIGIN      
        1 acgcggcaaa gactctggaa gcgttcggcg cccgtctgcg cgacgagacg gatctggacg
       61 acttgagcgg ccatctcgtg gaggtagtgc gggagacaat gcagcccgcg cacgcctcgc
      121 tgtggctgcg gtcttttgaa cggcgctccg agagtaagag gagcgagcgt ggataaccga
      181 cgtgcaacgg aacaggcaga gcggcctgga actcttccgc cctcgacgct gcgcggccgc
      241 tggcttacct tcgcgcaggt ggcgtgggtg gccgtggccc ttatcaccct ggggctcgac
      301 gcgatcagca ttccctacta ctatgcgcgg gatagggccg tgtgcgccgc gtgcgcgggg
      361 gattccggca ggatcactcc ggataggctc cgggcgctgc acgatatggg cctctcggcc
      421 ggcttctacg ccggctttga cacggcggtc gagatcatcg ccgtgctggt gttcgcagtg
      481 gtcgcggccg tgatcttcct gcaccgttca cacgaccgga tggcgctgtt cggctcattc
      541 acactcctgg tattcggcgg cgccgcgttc agcagcgacc tcttgaaagg gctggtggcg
      601 gcgcaccccg tattccgctt cccgacggag cttctggatt acatcgggca ggtgtgcttt
      661 atcaccttac tctatgtctt cccggatggc cggttcgctc cgcgttggac gcgctggctc
      721 gccgttgtgt cggcgctatt gtgggttccc accatattct tttcagcctc gtccctggat
      781 ctcctgagcg gtccctacat cgtcgggctg gtaggtgcgg caatggtcgc gcaggtctac
      841 cgctaccgta aggtatcgag tcctgctcag cgccagcaga ccaagtgggt ggtcttcggt
      901 gtggtggtag ccataactgg gttttcgggg atgatcacgt tcagcaatct cgtcccgagt
      961 gtccaacggg cggggccgct ggtgcagatg ggggccaccg ctttcatcaa cgggttcctg
     1021 ctgctgatac cgctctcaat aggcgtcgcg atggtgcgtt cgcggctcta cgacatagac
     1081 gtggtcatca accgcaccct cgtctacgcc gcgctcacca tctcgctggc atccgtctac
     1141 ttgggcgtcg tcgtcgctct gcagtacatc ttccgcgccc tcgccggcga gacttcccag
     1201 atcgccgtgg tcgcctcgac cctggtcatc gccgcgctgt tcaacccctt gcgccgccgg
     1261 gtacaggcgc tggtcgaccg ccgtttctat cgaaaaaagt acgacgcggc gaagaccctt
     1321 gaggctttca atgtccggtt gcgggacgag atggacctcg ggcagttgag tagagacttt
     1381 atatcagtcg tcgaagatac catgcaaccg gagcatgtat cgctgtggct gaggccgcca
     1441 gaaaataagt gagggaccgc gaagttcccg gatagacagg agaggatctc accattccac
     1501 cagcttccag atctcgggcc gaagcgctcc ttcctccccg atctcggcga agatcgccac
     1561 ggcctccttt aggtgcgaca tggactcttc ctgccgccca gcggtgtgga gcaggtcggc
     1621 gaggttgttg cgcagcgccg cctcgcgatg gcggtctcct agagagacac acagcgcgag
     1681 ggcggtcccg gccaggtcca tcgcctcctc gacttcttca cccctgccaa gagccagagc
     1741 gaggttgttg agcgccgcga ttcgagcttc tggatcgttc aggctctccg cgagcgccag
     1801 actgcgttcc agacgccggc gcgcctcgcc gaagtctccc ctactactcg ccaggatgcc
     1861 cagagcgttg tgagcctgga cgagcgcgcg ctcatcgtcc gcctcttcgg cgagatccaa
     1921 cgcccgccgc gcgaactcgg atgcagcttc gtcctgaccc tgacggtggg cgagcaggct
     1981 ccggtcggcg tagagtctgg cacgctccgg tgagccgtcc tcccctagcg cctccagagc
     2041 cgccgcgtaa tgggactcgg ccagttccca ctcccctcgc ctcccgtgga cgtcgcccag
     2101 cttgcgctcg ataccggcca gatcttcccc ggcactgagc gcggcggccg tctcgtagct
     2161 cgcgagcgct tgttcgtact ctccggtgag cgtgcggagg tcgccgatgg ccgcgtgcag
     2221 ggcggcggca tcaggatacc cgagggcgag cgccgtctga taatgcgaga gcgcctcgcc
     2281 gttggcatag agcgcgcggg catgctcccc ggccagcttc gcgtactccg ccgcctcggc
     2341 gtcctgcccg gccatccggt agtgatagga gatctggccg gccagagagc cgccctctct
     2401 gcgccggtcc gcgcggtcgg cgagagcggt cgctatgcgc tggtgcagca gtcgcttccg
     2461 ggccaggctg gtcttttcgt agacgatctc ccgcagcttc tcgtgggaga agtcgtagac
     2521 cggatagccc tctttggcac tctcggggac ctcccggatc aagaaccgcg accccaactc
     2581 ctcgatggcc gcgatggtct cctcctcact gcggccgctg gcctcgcgcg cggtgtcgaa
     2641 atcgaacgag cgcccgatgg ccgcggccgc ggcgagcaac tgcttcccgg tctcgttcaa
     2701 cgattgcaag cgtccttcta acaggtcccg cactccgccc ggcagcgacc atgcgccgct
     2761 ttcgggacca agctctcccc gaacggcagc ggcgaggtac tccgtaagga agagcggcag
     2821 cccctccgtc tcgtcgtaga gtcgccgctc cagctcctcg tcctcgcccg cgacggaacg
     2881 taccagctcc cttacggaag gagcctccag ccgtccaagc tccaggatcc tggcgtgccc
     2941 ggcgcgttga gcctcgcgca cgagcgcccg caagcggtga ccctccggca cctcctcctc
     3001 acgccacgcc acgagcacgc gaagcggctt cgcgtccagc cggcggacca ggtaggacag
     3061 caagtcgagg gacgcatcgt cggcccattg caggtcgtcc aggaatagga taccgctcgg
     3121 ctcgcctccg cagaccgcca gaagcacccg gctcactccc tcgaagaagc ggctctgggc
     3181 gcccggcgcg tcgagcggcg gcgcgtccgg caggtccggg aagagcccgg cgagtccggg
     3241 aagtagccgc ctcgcctcgc tcaacagata gtccggtatc tcctccagcc gggaggcgac
     3301 gtccgtctga cccagcgcgg actccagggc ctcggcgaag ggaccgtagg ccaggttcgt
     3361 ctctcccgcg taacagcgag cggcgagcgc gaccgcgccg ctctcccgga cgtgggccac
     3421 gaactcctcg gcgaggcgcg tcttgccgat accggcctcg ccctccagca ccacgacgta
     3481 tcctcgcgcc tcgggtccgt aagcctttcg caggtcctcc cactccctcg ctcgtccgac
     3541 catcggactg ccggagaacc ggacctcgga ggcgctctcg ggctggagcg tgtccgcctc
     3601 cggtaccgtg tggatggaga acggcggcgg cgttcctcct tgctcgatgg tccggtagag
     3661 tccggtcgtc tccgccagcg gcgggacgcc gagctccttt tcgagcgtcc gcacgcactc
     3721 gcggtactgg cgcagggcgt cggcgcgctg cccggagagg gcgtatgcct gcatcagcgc
     3781 ccggtgagcg gactcgctca ggggatccag ggcgagcctg cgccgggcat acgcgatggc
     3841 ctgctcccac tcgtttcgcg cgccatagcc tcgcgcgagc cgctccagcg cgccgcccaa
     3901 ctcgcggcgc aggctctcgg cctgaaaata ttgccagtcg tcgaagtcga agctgtccct
     3961 gagaccgaag ccggccagga agtcgccgtg gtagagcccg gccgcctcgg acaaaatggg
     4021 cagacacgcc gcacacacct cggtctctgg atgtccgtgc gtccggcatc gttcgagaag
     4081 ctcgcggaac cggacggcgt cgatccaggt gccatcccgg ttcagggcag cgctctcccg
     4141 atccacctcc agggggcctg ccgaccggag accgctcaga gccgagagcg tgcggcgcag
     4201 agcggcgcgg gacttgctct ggctgtactc cggccataga agaccggcca gggcatcgcg
     4261 gccgtggcgc ccctcctcaa tagacaggta ggccagaaga gccaccgcct tgcgcgtatc
     4321 cacctcgatc accgcgcccg catgttcgat gcgtggcgaa cccaggagaa acaacctggt
     4381 gtctggcacc ctctcacctc ccattccagc catagattat gctccgcgcc gctcgctatc
     4441 gcaacggttc ctgagttggg gcaccaatcg taacgatgcc acccccaagc ggcggaacaa
     4501 gctttcagag agcgtttaca agaaaagaga aggtagattc tccaaaactc tctcccgtaa
     4561 cgcttttcgt aacggttagg agacggtcgc ccggtaggat cgaagcgaag acttacaggg
     4621 aagcgggaat tggaaccgct tccggtttca agaaggagga agtaaaagat gatgaagatc
     4681 gacggcggtt tgaggatgtg ggcggcggtg ctggtggcgc tggcggcggg gttggtcctg
     4741 gtggcggcgc agtccgctcg ggcgggcgct ttccctggag tcaacggcaa gatcgccttc
     4801 gtcagcaacc gcgacggtaa cgatgagatc tacaccatga accccgacgg caccgggttg
     4861 aggcaactca ccaggaccct ctacccgtcg ggcaatgagt ctcccgccgt ctcgcccgac
     4921 ggcagccgga tcgtcttcga gaggaacaag gacgtctgga tcatgaacgc cgatggtagc
     4981 ggactcaggc agttgactac cgatggggct ttcgaccacg atccgacctt ctcacccagc
     5041 ggcgccaaga tcgccttcct gacccgccgg ggaacagacg ccccgatctc gcgggacaac
     5101 atctggacca tgaacaccga tggcgccaat cagaccagag taacttccaa cggggccatc
     5161 gagccgacct ggtccccgaa ttccgacgtg atcgccttcg tgagggccgg agcgctctgg
     5221 gtaacgaacc tgaccaccaa caccgagacc cagtacacct tcaccaacac cggcaaccca
     5281 gatacctcgg tgggcggggt agccttcccc acctggtctc ccaacggcag cctcatagag
     5341 ttcaccggca taggaggagc tctccactgc tcctgctcgg gttacttctc cgtgagcgcc
     5401 gcaggaggcg cgcccaccac cataggaccc gacgatccca gcgacaagtt cctcgccttc
     5461 tcgcccgacg gcgccaggat cgcacttcag agcaccaacc gcgagcgcaa cgaagagatc
     5521 tacaccatga accccgacgg ctccggcctg acaaacatct cccacgacct ctcgttcacg
     5581 gacaaccagc ccgactgggg accgcaggtt acgcagcaga gcaccagcct gagcctctcc
     5641 gccaatcccg cgaccatcac ctccggccat agctccaccc tctccggcag gctcgttacc
     5701 gccagcggca aagtctacgc cgggcagagg gtgaccatcc agcagaagcc gaagggagcc
     5761 agccgcttca gcaacctaaa ggcggtctcg actgccttcg acggacggtt caagacgacg
     5821 gtcaagccca agaagacaac ctactaccgg gcggtctttg gcggcaacgg cgtgcttggg
     5881 ctcggcgctt ctacaagtcc cgccaggctc gtgaaggtaa gtgggtagag ggcgctgaag
     5941 ccgggctaga tgcacgatat gagaaacatg ctaacgaaga ggatacaggc ggctgctcct
     6001 gctggcaacg tgagcccggc caggaagctc aaggtgtcca ggtaagcggt tatatgctcc
     6061 agataccaag tgtacgagag gtggtgccgt ccgcgttata cgcaccgcct ctcgcacaga
     6121 ggagagcggc gcagtcaaat gatcaggcta ccgtgagaaa cgcctctcca aagcccgcca
     6181 agatcaagga caggacgcca gccataaagg ctaccgtcga gaacaacgta accaacctca
     6241 agaaggcgta cctaaaaccg tatgtggccg gcaagcgcac ggcaaacagc aagttccgct
     6301 acagtgcgtc taacgcaatc tcctctacaa ctcgctcagg cccacgaagg gcgagaaggt
     6361 ggcaaagata gtagccgggg atgcggcggg caacgtgaga gcgaagagct gatgcttcaa
     6421 gatcgagtag agaatagtcc gatcgcacat tagcaaatac tgagaagagc cgggaccgtc
     6481 cataaggcgg cgacaggtcc acaaggtgcc cgcgggccgg gcaaccgtta agatagatca
     6541 gacaattcag aagtcagcga gaagcgggcg gggctttgtc ctttggaaac gccatactgg
     6601 atgccataga ggtcaagaag tttgacggta cactctcgga ggcggttatc cgagaggtcg
     6661 tcgagggcta tacctccggc gccgtgccgg attatcaaat gtcggcgctc ttaatggcgg
     6721 tcttcatccg ggggatggat tacggggaga cgctcgccct cacgcgggca atggccgagt
     6781 ctggagatac ctactctttc ccggactgtg tcgacaagca ctcgacgggt ggtgtaggag
     6841 acaagatcag cctgacgtct ctcccaatcg tggcggcgtg cggcgcgccc gtagccaagc
     6901 tctccggacg cggcctcggc acgaccggcg gcacgataga taaactggag tccatcccag
     6961 gcttctcggt ggacctttcg agggcgcaat tcgagcggca ggtggcggag gtcgggctgg
     7021 cgatcatcga ggcgggtgag ttggcgccgg cggacaagga gatctacgcc ctgcgcgaca
     7081 cgaccggcac ggttgactcc ttgccgctca tcggctcctc gatagtctcc aagaaggccg
     7141 caaccggcgc gggacatctg ctctacgacg tgaagtgcgg ctccggcgca ttcatgaaga
     7201 ccctggaaga cgcccgagag ttggccgggc tcctggtgcg cctgagcggc tctttaggca
     7261 tccgggcgtc cgccctcata acggacatgg acaacccgct cggctccgcc atcggcaacg
     7321 cgctcgaagt gcgcgagagc gtccgcttcc tgaagggcga gcacgtcccg gaggacctcg
     7381 cgcacgaggc ccgcctcgtt gccgtccgtc tcctggaact aaaaggcatc ccggatcccg
     7441 aacgagcggt cgaagaagct ctctcctccg gcgcggccta cgagaagctg cgcgagttcg
     7501 tccgtgccca gggcggcgac gcagatgctc tggatgacct gccgatatcg gacgaagttc
     7561 gggaggtcgc tgcgccgcgc gccgggtacg tcgtccggtt cggcgccatg ggcgtcgggc
     7621 gcgccgcgct gatgctggga gcgggccggg agaagaaggg cgactccata gacttcggcg
     7681 ccggggtgga ggtgctggtc gggatcgggg ataaggtgga agaaggccag cccgtggccc
     7741 gtctctacgg cgcccgccgg acggagcgcg cagagcggct cgtgctggaa gcgctcgaac
     7801 tctccggcga gcccgtcgag cctccccctg ctatcctgga cagcctgtga gcacaagccg
     7861 ccagccagga gtgaagagcc gaacgctggc ggcagaagcc ggaagttaat aagaccgaag
     7921 ggagcgacag atgagtccag tccatgtgcg ggccgagccg ggggatttcg cggagagcgt
     7981 gttgttgccg ggagaccccc gccgggctaa atacatcgcg gagaacttct tcgaggacgc
     8041 gaagctcgtg accgaggagc gcgggatgct cggttacacc gggacttaca agggagagcc
     8101 ggtctcggtg cagacgacgg ggatgggttg tcccagcgcc tccatcgtga cggaggagtt
     8161 gatccagctc gggtgcaaga acctcctgcg cgtcgggacg tgcggcgggt accacgagga
     8221 cctgcggctg ggagacctga tcgtcgcgac cgccgccacg ccgcaggacg gcacggtatc
     8281 ctcgatcacg cagggcgtcc cctacgctcc agcggcccat ttcgacctcg tccacgccgc
     8341 ccatcacgcc gcggagtcgg ctggcaggcg gacgttcatg ggccccatag tgagctcgga
     8401 cctgttctac gatccggagg acgacccggc aacgttatgg agcaacctgg gggttctcgc
     8461 ggtggagatg gaggccgcgg cgatcttcac gatagccgcc atgcgcggcg tgcgcgccgg
     8521 gtgcctgctc accgtctccg acaccatagg ccgggaagcg gtggtgcgga tagaggactc
     8581 agccctccgg gatgccgtgg acaacatgat ggccctggcg ctcgacaccc tgaacgcctt
     8641 aaactagtaa gctgtcgggg aagagcggcg agccggaaga ggtagcccgg cccgcggcgc
     8701 gggtaggacc tcgggaggag tctcaggagg gcttttgacg atcctgaacg agtcaggggt
     8761 agcggacact ctctcgcccc ttcacgcctt gagcgggctg gcggagaagg tgccgggcgc
     8821 cgtcatagcc gtgatcggga cccgcgccga agccttcctg gctcaatccc tcgacgccgc
     8881 catcccggac cgctccccgg ctatccgccg cggcccgcga gtggtcttcg tcgtcctgga
     8941 cccggcccat ccgccggagg gcggacggat agcgtcccgc ctcatcgcgg aggttgcggg
     9001 catcagcggt accaggatgc tgctgctcgt cgccggccgc tccgccgaac tcctcggcgt
     9061 tgacgccaac ttcgaggctc gtctggcctc ccggcggctg gacatcccgg cggcggccgt
     9121 cgccctggac ccggacggca actctcccgg ctccctctcg accgaccttg aggaccgtgc
     9181 cctggcggcg ctggtcagcc tctgccctac cggcacctcg gagaacctgg agctgccagc
     9241 taccgccccc aagcgcggcg gcctcctcgg gaacttcctc ggacgcgggc gggaggagac
     9301 ccgctccggc caccggaagg cggtggtctt gctcgggtcc tccggcgagg aactggcgag
     9361 agagctggag cgagccgggg tcgaggtagc agggagcgtc cccggaccag atgccaccgt
     9421 actgccggac atcggagagg gtaccatcgt cgccctcact gacccgcacc tcactgccgc
     9481 ctcgcgagcg gcggaggaga ggggagcgca gatcgtgcgg acgctcacgc ccatcggggt
     9541 ggatggaacg gcgcgcttta ttcaggacgc ggcggcggcg gcgggaacgg ttgcgaacga
     9601 gctgggccgg gcgcgggagg cgtgggagga gatggagcac ctgcggaacc gggtgcgggg
     9661 caagagggtc ttcttcgccg gggacacggg atacgagata ccgctcgccc gctttttggc
     9721 cgacgccggg gcggttgttc tggaggtcgg ggcgccgcgg ctcgaccggc gggggttggg
     9781 ggccgagatc caggcgatgg gcgcggacgt ggacatcgtc cagtacccgg actggcgagg
     9841 tcagatccaa cgcgccgacg agacgcgccc ggacgtggtg atcacatctc ccggcctcta
     9901 cgcgccgctc gtcgcccgcg gccacctctg ccgctgcgcc cgtgacttcc tgaccgccgg
     9961 gccgcacggc tatgagggag cccggcgcat cctggagact ctcattcgca ccttcgagcg
    10021 ggccgagctg ctggactccg taaacctgtg agcgcctttg gacccgtggg taacgcatgc
    10081 ggctgatgcg cggcgtctac gaaggtccgg gcagccacgg catcctgcgc gtggcggcga
    10141 gcatgacgaa cgtccacgcc gtgctgcggg cgctcccgca cgagggatat ttccacgctc
    10201 tctatggcgc gtggtcgcgc agcggcgagg ccccgcccgt gacgctctcg cccgtctggg
    10261 gtgacagcgc gaagcaggaa gcctcgatgg cgccgggaca cctgcggcgg gatctctcgc
    10321 aggtcctgcg ccgccatccg ggcacggaga gcgtcatcct cgcccgctcc gaagccgcac
    10381 tgctctctgg agaggagatc cccacggacg ccctccccga gaacccggag accggcctca
    10441 caccgggcct cgtcgcctgc gactgggagt ctccgcttgt gcgggagatc gaagccgccg
    10501 acctcgccct cgaagacctg gtccgggcgc acgcgaaggg gcgcgagcgc agccccgacc
    10561 cgaccgtcaa catcttcgga ccgccaatct tctcgccggg cgccgccgcg gagtacgacg
    10621 aagccgaacg gctccttgac ctgatcgggg tccaggtcaa cgctcgcgtg ccgttggatg
    10681 ccggtgtccg ggacctggca cgcctgccgc gcgcctgggc gaaccttgtc ctgtaccggg
    10741 agacctgcga gtcggcgacg ctgtatcttc aagacgagtt cggcacgcag cgggttacgc
    10801 cgcccatgat cggctccgcc ggcaccggcg cggcgctgag gtcggtggga gagctgtgcg
    10861 gcctggaacc ccagagggtg cagcgagtcg tgtgggccga gctctcccgc acctcccggc
    10921 ttccctggta cgcccggctc gccaccccgg agactttcga ggaccgccgc gtcgccatct
    10981 tcggagactt cacttacacc ctgggtctcg gttacgccct ggcgcgcgag gtcggcctgg
    11041 aggtcgcgcc ctgcggcacc tacctcaccc acctcgaacg ggacttcctg ttccacgccc
    11101 ataccttcac cgaggacgcc ttcgtcaccg acgacccgga cgaggtcgcg gaccggatcg
    11161 aagcgtcccg gccggacctg ctcatcggca cccacctcga aggagcggtc gccgaggcgc
    11221 tgaacatccc gttcctgcct ctatgcccgc ccgtcacgac gcacccgttc gtgcagcgcc
    11281 cgctgatggg atacgcgggc tccagcgtgc tcgccgacgc cctggagcgt tccccgcgct
    11341 ggacggaaga gaagcccagg gccatccccg ccgggctccc gtggaccgat gacgcgctgg
    11401 ataagctgga ggagatcccg gccttcctcc gcggccgagc ccggcggatg gccgaggaac
    11461 gtgcccgctg gctcgacgcg ccggaggtaa cgcccgagat cctggccgaa gcccgccgct
    11521 gaaccgccgc gccgtcccgc tataatccct ggaatgggaa tcagagactt tatcgagtac
    11581 ctgcgggaac accaggagct tctgcgaaac ggcgcggagt tgccggaggc ggagcgcgag
    11641 gagacgctgc gggaagcgga ggtaatggtc ggaatggccg aggcggcggt caagcagttg
    11701 gagtccgtga tcaagaaccg ccgcgtgaac ctcccagagg agcacgccgt ccgcttcgcc
    11761 caggacatct tccgccacct catcgccgaa gaagagaagg taaaggagaa gctgcgcgag
    11821 caagtctcgc cgtgatcttg cctcccctac tgcgcgagga cgacactatc tgcgccgtcc
    11881 ccctcaccct cgcataagtc attattcctc cggtccagac tggggcggaa accggtgagt
    11941 cggccgaaac tgtttggggc ggcgttcgta taggtcttca gttgagatta gcgctggaag
    12001 gtctccggcg ccgacaggag gtcaggaagt cttatgttga agtccaggat ctactttctc
    12061 ccgttgctcg cggccctggc agccgtgttg ttcgtggcgg gatgttcact acagaacggc
    12121 cagggaaaca aggcagagaa cgtcgcccac aaacccgccc cggcctcgcc gaagataccc
    12181 gagaccgaac cggtggcccg ggtggcttca caggtcgagc cgagcgtcgt gcaggtcaac
    12241 gtgcgttcca tacagatgac gccgttcggg aatcaggccc aggagggtct tgggagcggt
    12301 gtgatctacc gcaaggacgg atacatcatc acgaacaatc acgtggtcca gggcgcgagc
    12361 caggtgaacg tcgccttcgc cgacggttcc acggaacgcg gacaggtggt cggtacggac
    12421 ccgaccaccg atatcgccgt ggtcaaggtg aatcgcaaca acctcccggc ggcgaagttc
    12481 cgcaagagcg agaacctcgt ccccggccag ctcgccgttg ctatcggcag cccctcgggc
    12541 ttccagtcca ccgtcaccgc aggcatcatc agcggcctta accgggagat acccgcctcg
    12601 ttgaccggag gcaccgatac ctccctggtg gacctgatcc agaccgacgc cgccatctct
    12661 ccggggaact ccggcggggc gctcgtggac cgcgccggtg agatcatcgg catcaacgtg
    12721 gcctacctgc cccagaccca gaccggccag ctagtcgaag gcatagggtt cgccatcccg
    12781 tcggacaccg cagtctccgt cgccgaccag ctcatcgcca acggcaaggc tgttcacccc
    12841 tacctcggca tccagctcgc ggacctcacg cccgaagccg ccaagcagtt caacctgaag
    12901 gtggactcag gagtcgtcgt caggaatgtc gtatcaggct ccgcggcgtc caatgccggg
    12961 gtgcgctcgg gagacatcat caccgccatc gacaacagga agatagcgga ctccggagac
    13021 ctgctcggcg acctgcgcga ctataagcca ggggatcagg tcaagatcac catcgtaaga
    13081 aacggcaaca agaagacact gaacgtcacc ctcgaccagg cgccgcaaca gcagcagcaa
    13141 caataacgaa ccaattaccg agcagaagaa gggactccgt gcggggcccc ttcttcttcg
    13201 cgcctctgcc cacatctttt attgaataat tcttcaacta tgcatataat atcggtatgg
    13261 cgcatttcaa gacaacttcg gcggacgtaa ggttattaga accacgggac gtactggatg
    13321 gtggcgaatt tgctggcata gacgagcggg tggcgggtct catgggggcc atggccgggc
    13381 cgacgcgcat ccgggcgctg ttcgcacttc tcgatgcggg tgagcttccg gcgggcgagg
    13441 tggcgaaggc ggttcggatg agcgcttcgg cgacgagcca ccagttgcgg gtcttgcgcg
    13501 acctgggtct cgtgaatcgg cggcgggagg gccggaagtg tttctacgcg ctcgccgacg
    13561 agcatctcgg ggtgctctta aaagaggctc tgtaccatgt ggatcacacc cggctcgcgg
    13621 aacaggcagc cggtggcaga ggcgcgagcc gttgaagcgc tcgcgcagtc atccggcggg
    13681 acatcagcac gagaccggaa ggtgggcgaa ggtatggcat acgctctccc acctcatacc
    13741 tttcgggcat tcgcacgaga gttcggagag cgtggacgac gcgctggagt cgagcaaggc
    13801 cgggatacgg gcgctcaaga tatctctagt ggcgttgatg ctcacggcgc tcatgcaggc
    13861 cggggtcgtt gtcctctccg gctcggtcgc gctgctggcc gacacgatcc acaacttctc
    13921 cgacgccctt acggcgctgc cgctgtggct cgcgttcgct ctctcgcgcc gggccacgaa
    13981 caggaggtac acctacgggt acgggagggc cgaggacatc gccggcctgg ctatcgtggg
    14041 gttcatcttc ttcagcgcct gcgtggcggg ttacgagtcg gtgatacgtc tcatctcggg
    14101 ctccgaggtc tcgaacctcg gctgggtcgc cgtggcggcc ggcataggtt tcgtgggcaa
    14161 cgagttcgtg gcgcagttcc gcattggggt cggccggcgc atcgggagcg cggccctcgt
    14221 ggccgatgga cagcacgcgc gggtggacgg tttcacgtcg ctcgccgtcc tcctcggcgc
    14281 cgcgggtgta gcgctcgggt acccggtcct ggaccctctt atcggcctgc ttataacggt
    14341 cgccatactg ttcatcgtca aggactccgc ccgctccgtg tggcgccgga tgatggacgc
    14401 cgtggagccc gagatcgtct ccggcatcga ggaggccgca gcgagcacgg agggggtaga
    14461 gaaagtcgag tccgtccgcg cccgctgggt cggccaccgc atacactccg aggtgcgtat
    14521 ccgggtagac ggcggcctga gcgtggacga gggagaggag atctctgggc gggtccggga
    14581 cgcagtccgc cgggcgaccc ccagactcga ccgcgtagcc gtggagattg gggccctgta
    14641 ggcattcctg ggttagactg gagtcctgta tggcacacga ccacacccat cacaaccacg
    14701 gagaccacgg cggcgacggg cactctcacg gggtgagcga ggacgccgac cggcgctacc
    14761 tggcgatagc cctggctctg ctggtcgggt tcatgctggt cgaggtcgtc gtcggcatcg
    14821 tggcgtcgtc tctggccctg atctccgacg ccgggcacat gctgaccgat gctggagcga
    14881 taggtctcgc gctcgtcacg atgaggctgg cccagcgtcc cgcgcggggc gtgatgacct
    14941 acgggctcaa acgagcggag atcctctccg cccaggtcaa cggtataacg ctcctcttgc
    15001 tctccgcatg gttcgtctac gaagccataa ggcgtctcgt tcacccgcct gaggtggagg
    15061 gcgggttggt cctcggcatt gcccttctag gcatcgtggt aaacctgctc gcaacatggt
    15121 cgatcagccg ggccaaccgt cagagcctca acgtagaggg cgcctttcag cacatcctga
    15181 cagatctatt cgcgtttata gccaccgccg tcgccggagc cgtcatctac ttcaccggcg
    15241 gctacaaccg ggtggacgcc atcgcagctc tggtcgtagc ggctctcatg ttcaaggccg
    15301 gatacagtct ggtcagagac tcgggacgca tcttcctcga agcagccccg aaggggtttg
    15361 atccggacga gataggaagc gtcctggcct cgcgccccgg tgtcgcggag gtccacgacc
    15421 tgcacgtctg gactatcacc tccgggtttc ccgccctctc cgcccacgtc ctggtcggcc
    15481 gcgacgagga ctgccacgcc cgccgccgcg acctggaaga gatactcgcc gaagaattcg
    15541 acatctccca caccaccctc cagatggacc acgtcggaga ccaccacacc ggcaacacga
    15601 accttcagtt cttccccgac atccagcggc gcggcgaagg gaagtagagg tcggaaagta
    15661 gagacctgcc ggagaaggcc cagagttccg aagccggcgg catctacggt aagatagttg
    15721 cggctctgat cgagccagaa gaactcgaag aagagaggta cgccgatggg taagcagcgc
    15781 ccgggcagtc ggacgacgcc ccgcaggttg gacaacgcgg gacgcgagcg tccctggggc
    15841 ctgataatcg tgggcggtat cctggcgctg gtgctcatcg ccttcgtcac ggtggtggtc
    15901 ctggacagca gggagcaggc cgcgcgcacc ccgccaagcg aggtaaagac ttataaggtc
    15961 gaacgcggtc acacggagag caacgtgaag tatgcccagt cgccgccggt cggaggccag
    16021 cacaaccccg tgtggcagaa ctgcggcttc tactcccagc cggtccgtaa cgagaacgcc
    16081 gtacactccc tggagcacgg agctgtctgg atcacttacc agccggacct cccgaaggac
    16141 caggtcaaca cgatcaagga cctgaccgag agccaggact acatcctcgc cagcccgtac
    16201 cctggtctgg actccccggt agtcgcctcg gcctggggca agcagatcaa actgcagagc
    16261 gcgaacgact ccggcctgaa gcagttcatc agcgcgtacc gcaagggccc gcagaccccg
    16321 gagcccggcg cgtcttgcac gaacggcacc ggccaaccgg gctagcgcta catgaagtcc
    16381 gcctcacgac gctcggcacg ccgcccggac ccccgcacgc tcgtagccct gatcgccgtc
    16441 ctgctggcgg cgacgatgct ggtcatgtac atagccggcc gcccgccggg cgacggctcg
    16501 ccggaagccg ggttcgcccg cgatatgtcc gtccatcacg gacaggcggt cgagatggcc
    16561 ggcatcgtcc aactccgcac gaaggacccg aagatccgct cgctcgccac cgacatcgtc
    16621 ctcacccaac agtcccagat cgggacaatg cagggctggc tggacgtgtg gggcctctcc
    16681 ccgaccggca cggagcaaca gatggcctgg atgggccacc cgatgtccgg caggatgccc
    16741 ggcatggcga gcccgaaaga gatcaaccgc ctcagcaccc tcccgccgga gaagaccgac
    16801 gtgctgttcc tgcaactcat gatcccgcac cacaaggcag ccatcccgat ggcccaggcc
    16861 gtcctcgacc gcacaaacca acccgaagtc cagcgcctcg ccgactccat catcgcctcc
    16921 cagaagtccg agattgaagc catgcaagcc atgctcaaag acaaaaacgc ctctccggcc
    16981 aaagaggatc cgaagatgga catgaagtcc atggaccact aacaactcgc agcaacatat
    17041 caacgtgtgt tagtatgatt ttatgaacga tgagcgttgc gacctgttgt gcatagacgc
    17101 tccgctggcg gagggtattc ggacccggtt gcctggagtg gaggcagcgc gggctgcggc
    17161 ggagcgggcg ggggcgctct cggacccgac acggctcttg ctggcggtgg cgctcgctga
    17221 gggtggggag ttgtgcgtgt gcgacctcgc ctggatctcc gcccgtgctc agaacctcgt
    17281 ctcgcatcac ctgcgccttc tgcgctccca tgaactcgtc acctcccggc gcgacggcaa
    17341 gctcgtcatg tactcgctca cgaatacagg acgctcgctc ctcacggccg tgatgggcga
    17401 agtcagggta ggccagtgag cggtcagccg atacagattc agggccgtag gccgatgagg
    17461 tccgcggagc gtgaggggct cgttcgccgg gcgaagctgc tcgcctggct gggggtcggc
    17521 tggcacggcg tcgaggcggc ggtcgccatc ggggcggggg ccgtggccgg gtccatcgcg
    17581 ctcgtcggct tcggcgcgga ctcgctggtc gagtcggtgg cggggttcgt cctgctgtgg
    17641 cgcttcgcgg cttcgagagc atcatccgag agcgccgagc gccgggcgca gaagctcatc
    17701 gccgtcagct tctacgtcat agccgcatac gtgggcttcg aggcggtgcg tacactgctc
    17761 ggcgcggagc agccggatgc gagttgggtc ggcatcgggc tcgccgcgtt caccctgctc
    17821 accatgcccc cgctcgccat cgcgaaggct cgcgtcggtg agaagctcgg ttcttcggcg
    17881 acgaagagtg agggccgcca gaacatgctc tgcgcctacc tctcggcggc tctgctagta
    17941 gggctcggag caaacgcgct cttcgggctg tggtgggcgg acccggtgac ggccatactg
    18001 atctccgtcg tagcgataaa agagggccgg gactcgtggc gcggagagag ttgctgtacg
    18061 gcccccgtga ttgccggaga ggactgcgaa gacgactgct gccggtaaga gaggaacgtc
    18121 aatacgcgac cgtgacctgg acgcgctacc tatcgcctgc aacctgaccg ggcgggaagc
    18181 gagccagcgc aaggagtcca ccatcgaagt cctgcggcgg gcggagcagg cgcgggagat
    18241 agaagacggc tacgagctcc tgtttccggg agatgatgag atggtccgcg gcatcacgga
    18301 agtcgtgatc gccgagaggt cgtgctgtcc gttcctcgca ttcgagaccc tcttcgagcc
    18361 cgacggcggg cctgttctgc tgcgtgtctc gggaccgccg ggcgcgaagg acttcatccg
    18421 ggacgagctg gcgatgacga ccatcgagga atgacgttca tcctggatcg gctaaagcga
    18481 tgggcgcgga agctcgagaa cgaagtgtac gctctctatc tggcctacaa ggaccccagg
    18541 gttccctggt acgcgcgggc gctcgccgcc gtggtcgtcg gctacgcctt cagccccata
    18601 gacctcatcc cggacccgat ccccgtcctc ggctatctgg acgacgtcat cctcgttccc
    18661 ctcggcatcg cgctgaccat acgcttgatc ccggacgaag tgctggccga gtgccgcgag
    18721 aaggcgaaga cgatgcagga cagaccggtc aaccgggtag cggccgcgat agtggttgct
    18781 atctgggtgc tgctggtgat cgcggcgttc atgctcgtgc ggcgctactt ctgatccggg
    18841 ctatggcgga ggcaggctgc taaggaaacc cgtagccgcg aacacgccca gtgccatcag
    18901 cagctcggc
//
LOCUS       DASSMB010000060         9546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_141204, whole genome shotgun sequence.
ACCESSION   DASSMB010000060 DASSMB010000000
VERSION     DASSMB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9546
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141204"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFJ72_04185"
     CDS             complement(<1..226)
                     /locus_tag="VFJ72_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478699.1"
                     /translation="MRIAVVADDLYPGFGGQAAATEGHVEALLARGHEVRALAGFEPV
                     PSEPSPGVSVERLPVWRPGEKQTQLALPDSK"
     gene            complement(229..663)
                     /gene="dut"
                     /locus_tag="VFJ72_04190"
     CDS             complement(229..663)
                     /gene="dut"
                     /locus_tag="VFJ72_04190"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET7478700.1"
                     /translation="MTFRRLVPEAREPARAHEGDAGYDLRSIEEARLEPGARALVGTG
                     ISVAIPAGFAGLVLPRSGLAVRHGISLVNTPGLIDSGYRGEIKVPLINHDPADTFLVE
                     AGTRIAQLVLVRVAVAAFTEVESLEAGADGRGEGGFGSSGSD"
     gene            768..2495
                     /gene="metG"
                     /locus_tag="VFJ72_04195"
     CDS             768..2495
                     /gene="metG"
                     /locus_tag="VFJ72_04195"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7478701.1"
                     /translation="MSEKRERYLVTSALPYANGPLHVGQIVGAYLPADVFVRYLRARG
                     EDVVYVCGTDEHGVPITLTAEREGKDPQEVVDYWWKHMEKTFERFHISFDNFSRTSTP
                     LHAKNTQLFYHKLKEGGYISEAESKQMYSPTEGRFLPDRYVEGTCPVCGYKEARGDQC
                     DNCGSWYEAYELIEPHSKITGGPAEVRETMHLYLDLPKFSDRLKEYVGGQEHWRSQVK
                     NFILGIIENGLEKRPITRDIQWGVPVPEPGFEDKRFYVWFDACIGYVSSTMEWAENIG
                     DPDRWREYWQEPGTRLIHFIGKDNTVFHAIVWPAQLMGVGEDGEEPYVLPYDIPANEF
                     MNLEVVVDGEPRRMQMSTSRNLAVWLHEALDRFPADPLRFYLASNLPETGDVVFSWRE
                     FQSRVNSDLIGNLGNYINRVLSFTEKYFDGELPRPDALTDDAGEVFEDFKALEARYAE
                     KMLAPRPREALGELLAMGRRANRFFDAAAPWKSRKDDPEGAKAALYACCALLGSIAYH
                     AAPYVPESIERLREFFGGPVARVSDLDLPDSYRVSGAKPLFRRIEDDEIGAVEKQLSD
                     ALMGDGNQR"
     gene            complement(2516..3658)
                     /locus_tag="VFJ72_04200"
     CDS             complement(2516..3658)
                     /locus_tag="VFJ72_04200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478702.1"
                     /translation="MWRLLAVELGAFGFFGLWAGCFAVLLADLSRALDLSPGPLGVAL
                     FAGAAASILAMAALGWTADRLGRRAYLVAVSLVFGLGIIGVAFANGYAMLLAALIVLY
                     SATGLYDVGINAAAVDLEKTSGRRFMALLHAAFSSGGVAGALTAGALLQAGTYYSYVY
                     LMLLAPLTVVMLAVSLTRFPVPEPHVGGTEKARGLYRSLPLLLVAAIATLGMLSEGEM
                     EHWSGIYLRQTLELSALLGGSGVAVFYGAMAVGRLGATWTISRFGDRRTLLGAGLLTT
                     LGMALALATHWPLIVVAGFLVVGLALSSVVPIAFSVAGDLAPGRAGAAISVVTTLGYG
                     GFLLGPVIVGGLAELLGLRIALATIAVAGVVISTLALRVGGRPTRL"
     gene            complement(3672..4031)
                     /locus_tag="VFJ72_04205"
     CDS             complement(3672..4031)
                     /locus_tag="VFJ72_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7478703.1"
                     /translation="MAKSYEEIVSEAKAETEQVSVEDVRDALDSGESVTIVDVREPDE
                     WEEGHIPGARHVPRGILELQAEAQLPEKDARIVVHCALGGRGSLAAKSLQKMGYTNVA
                     NMEGGVNAWQERGYDTE"
     gene            complement(4061..6046)
                     /gene="thyX"
                     /locus_tag="VFJ72_04210"
     CDS             complement(4061..6046)
                     /gene="thyX"
                     /locus_tag="VFJ72_04210"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET7478704.1"
                     /translation="METTGRSAERVQELFESLGGSVNDGFPAIHSPVSHTGAGTAYLR
                     RPGVVMLAKPQVNVAGLAGFLEGFDPDLGFPGYLEDPTTLPGSSQLCKTAGQLCFDDE
                     TEILTDEGWKKFQNLHQGERVLTLNPDTGEAEFQQPLAYQVYDHDDDLFVCEGKALSF
                     AVTPDHRQWARLKDGGYEFYSTKEIWSRRFAVRTTSSGWVGEIPDRVRIPGFEFGQRI
                     CNQYGSYGSNSTIVSERVVESVEEIRALALLCVYYAAEGSSIVGRGEGVVVYGDHCEK
                     VSSLAETLGLKSSRWLDPRNDVPRTLVGGGRAIRTFFEQECGVGSARKRLPRWVLDLP
                     TNLLTEVWEILVATDGHRGESGRENFVTTSLEFAGQVQEILCKLGFSSSLMHKKTPEG
                     RLQVYIVSKRTRTEAWANKNARVERKTYSGKVYCVTTENGIVFVRRDGKPHFSGNCYA
                     SFGPRRTTNENAAAYFERLTSAGHGSVLEHASFSFLLYGISRSVTHELVRHRAGAGFS
                     QISQRYVSGSVLRFVERPEYQEDEELHRLFEERADRAAAEYEAMAGRLLEYQEGGSAM
                     LSADYKTDARKKVQQTARSLLPNETEAPMVFTGNVRALRHIIEMRADAHAESEIRNLA
                     VRVFLCLRTADPILFGDYTFGELPDGTVTVATEHRKA"
     gene            complement(6100..6948)
                     /locus_tag="VFJ72_04215"
     CDS             complement(6100..6948)
                     /locus_tag="VFJ72_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HET7478705.1"
                     /translation="MMQAQDSLTQYLGQIRDGRLLDRREEIELSRRVREGDMRARRML
                     IECNLRLVISIAKKYRGRGVAFEDLIQEGNAGLIRAVEKFDPEMGNRFSTYATWWIRQ
                     AVTRTIADHGRAVRLPAHVVDAVFRLRRAENALSVELGRDATEEELAARLDVKPEEAR
                     RLREVSQPVSSINARMGTGADDGSEMGELLADERSGEDYERVEIGSWEGALVEAVRGL
                     PEREAKVLQMRHGLDDGRIRTLREVSEVLGISQERARQVEIKALRTIRTGRYSSTLRR
                     ALSEAV"
     gene            complement(6983..7264)
                     /locus_tag="VFJ72_04220"
     CDS             complement(6983..7264)
                     /locus_tag="VFJ72_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478706.1"
                     /translation="MRKRSALRPDTTVAPAGEPHREVYVREIPTCCIRSLKEAALREP
                     GIRRSLSLACSCGREWWITSSLDERVLERFVTHGGPRVNGGFGDGPSAA"
     gene            complement(7261..7488)
                     /locus_tag="VFJ72_04225"
     CDS             complement(7261..7488)
                     /locus_tag="VFJ72_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478707.1"
                     /translation="MRSEARSERLWIAAMGEESVYEADLPACCQRSARIAEDGFECHS
                     CGAMWQAPIPVVPEEDAFMRRGSEDERRGAA"
     gene            complement(7491..8084)
                     /locus_tag="VFJ72_04230"
     CDS             complement(7491..8084)
                     /locus_tag="VFJ72_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478708.1"
                     /translation="MREEYMIERQGKRFVLYAGLLEEAHARGLRSIETDLLQIPAPEN
                     GEVAIVKATVRTEEGKYTGIGDASPENVNRAIAPHLIRMAETRAKARALRDAINVGVT
                     AFEELGGEESAVPAEETGVASGDGQRTEKQVRAPREEGKGELPATRKQLNYLEALIAD
                     VVEDGLARFEDMVGKPVGQLTREEASEWIGRLSGKAA"
     gene            complement(8164..8736)
                     /locus_tag="VFJ72_04235"
     CDS             complement(8164..8736)
                     /locus_tag="VFJ72_04235"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET7478709.1"
                     /translation="MTEDHSRLVYEVAGGDFGGKLMVTRSTGRAVREALGEALEGLPK
                     GGTLYVDTRGVELMDYSFADEALGVLVSRVAGGEYGDRRLVLVEEDRDMLENIEASLR
                     QRSLAMIRVDEVGGEPGIVGTVPDHLVETLEEIRAAGSITNADLAKKLGVNHTACNNR
                     ATRLAKLGLIHRKVETAAPGGRQYTYERIA"
     gene            complement(8832..>9546)
                     /locus_tag="VFJ72_04240"
     CDS             complement(8832..>9546)
                     /locus_tag="VFJ72_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7478710.1"
                     /translation="VDLALSGRDVPAYLERMDFFRHFGGRVKTNADLASLEERQRHNP
                     GTLQELINFHTEEEIPGIIHRISEILENQGYRVRERVAICSVLSEICANAAEHGRSSF
                     GAYAAVQAYHHVVSGTGRRGEEVLIAIADGGPGVRETLSRNPKYAEHTTTDNDALRHA
                     MMMGVSGTGELGRGGGLALVAQISGRAGGSLSLRSGTGRVTVYEDRKNSRNVPFFPGT
                     FVRVSLPRLAHDPREKSSR"
BASE COUNT         1607 a         3355 c         2945 g         1639 t
ORIGIN      
        1 ttttagagtc cgggagcgcc aactgcgtct gcttctctcc cggcctccac accggcagcc
       61 gctccacgct cacgccgggc gacggctcgg atggcacggg ctcgaaaccc gccagcgcac
      121 ggacctcgtg accgcgggcg agcaacgctt ctacatgccc ctcggtcgcg gcggcctgtc
      181 ctccgaaccc cggatacaga tcgtcggcga cgacggcgat gcgcacgctc agtcgcttcc
      241 cgaagatccg aaaccgccct cgccgcggcc atcggctccg gcttcgaggg actcgacctc
      301 ggtgaaagcc gcgacagcta cccggacgag gactagctgg gcgatacggg tcccggcttc
      361 gaccaggaag gtgtcggcgg ggtcgtggtt tatcaggggc acctttatct cgccgcggta
      421 gccggagtct atgaggccgg gggtgttgac gagtgagatg ccgtgacgga cagcgagacc
      481 gctacggggc agaacgagcc ccgcgaatcc ggcggggatg gcgaccgaga tgccggtccc
      541 gaccagcgcc cgtgcgccgg gctccagccg cgcctcctct atcgagcgca ggtcgtatcc
      601 ggcgtcccct tcgtgcgccc gcgcgggttc gcgagcctcg gggaccagac gccggaaagt
      661 cacctgtaac tcttgctcgg gagccatccg ggagatggta gcacttcgtc ccttcgggct
      721 gccggacgga cgccaagccg acttgccgac ctgctatcct tagctgcatg agtgagaaga
      781 gagagcggta cctggtaact tcggcgttgc cttacgcgaa cgggccgctg cacgtcggcc
      841 agatcgtggg cgcctacctg ccggcggacg tgttcgtgcg ctacctgcgc gcccgaggcg
      901 aggacgtagt ctacgtctgc ggcacggacg agcacggcgt ccccatcacc ctgaccgccg
      961 agcgcgaggg caaggacccg caggaggtcg tggactactg gtggaagcat atggagaaga
     1021 ccttcgagcg attccacatc tccttcgaca atttctcgcg cacctcgacg ccgctgcacg
     1081 cgaagaacac ccagctcttc taccacaagc tcaaggaggg cggctatatc tccgaggcgg
     1141 agagcaagca gatgtacagc ccgacagagg gccgtttcct gccggaccgc tacgtcgagg
     1201 ggacgtgtcc cgtgtgcggc tacaaggaag ctcggggcga ccagtgcgac aactgcggct
     1261 cgtggtacga ggcgtacgag ctcatcgagc cgcactccaa gatcacgggc ggaccggccg
     1321 aggtccggga gacgatgcac ctctacctcg acctgccgaa gttctccgat aggctgaagg
     1381 agtacgtcgg cgggcaggag cactggcgga gccaggtcaa gaactttatc ctggggatca
     1441 tagagaacgg gctggagaag cgcccgatca cacgcgacat ccagtggggc gtcccggtcc
     1501 ccgagccggg cttcgaggac aagcgcttct acgtctggtt cgacgcctgc atcggctacg
     1561 tctcctcgac gatggagtgg gccgagaaca taggagatcc ggacaggtgg cgcgagtact
     1621 ggcaggagcc gggtacgcgc ctgatccact ttatcggcaa ggacaacacg gtcttccacg
     1681 caatcgtctg gcccgcgcag ctcatgggcg ttggcgagga cggcgaggag ccatacgtcc
     1741 tcccctacga catcccggcc aacgagttca tgaaccttga agtggtcgtg gacggcgagc
     1801 cccgccggat gcagatgtcc accagccgaa acctggcggt gtggctgcac gaggcactcg
     1861 accgcttccc ggcggacccg ttgcggttct acctggcctc gaacctgccg gagaccggcg
     1921 acgtggtgtt ctcctggcgc gagttccaga gccgggtgaa ctcggacctc atcggcaacc
     1981 tcggcaacta cataaaccgc gtgctctcgt tcaccgagaa gtacttcgac ggcgagcttc
     2041 cgcgtccgga cgcgctgacc gacgacgccg gcgaggtctt cgaggacttc aaggcgcttg
     2101 aggcccgtta tgcggagaag atgctcgcgc cgaggccgcg cgaagcgctc ggagagctcc
     2161 tggcgatggg ccgccgggcg aaccgcttct tcgacgccgc cgctccctgg aagagccgca
     2221 aggacgaccc ggaaggggcg aaggccgcgc tctacgcctg ctgcgctctg ctcggctcca
     2281 tcgcctacca cgccgccccc tacgtcccgg agtccatcga gcggctgcga gagttcttcg
     2341 gcggtcccgt ggccagggta tcggacctcg acctccccga tagctaccgc gtcagcggcg
     2401 cgaagccgct gttccggcgg atcgaggacg acgagatcgg ggccgtcgag aaacagctct
     2461 cggacgcgct catgggcgac gggaaccagc gctaaatcgg cttcattggc ggcctttaca
     2521 accgggtcgg acgtccgccc acgcgcagcg caagtgtgga gatgacgacg cccgctacgg
     2581 ctatcgtcgc cagcgctatc cgcaacccca gcagttccgc cagcccgccg acgatgaccg
     2641 gcccgagcaa gaacccgccg tagccgaggg tggtgacgac ggagatcgcc gcgccggccc
     2701 ttcccggcgc gaggtcaccg gccaccgaga acgcgatggg cacgaccgac gagagcgcga
     2761 gccccaccac caggaatcca gcgaccacga tcagcggcca gtgggtagcc agcgcgagcg
     2821 ccatacctaa cgtcgtgagg agccccgcgc cgaggagcgt gcgccggtcc ccgaatcggg
     2881 agatggtcca ggtggcgccg agcctcccca cggccatcgc cccgtagaag acggccaccc
     2941 cggacccgcc gagaagcgca gagagttcga gcgtctgacg taaataaatg ccggaccagt
     3001 gctccatctc gccctcggag agcatgccca gcgtggctat cgccgccacg agtaggagcg
     3061 gcaggctgcg gtaaagccct cttgctttct ccgttccgcc cacatggggt tccggcacgg
     3121 ggaaccgcgt aagcgacacc gcgagcatca ctactgtcag cggggccagc aacatcaggt
     3181 agacgtaact gtagtacgtg ccggcctgga gcagcgcgcc ggccgtcagc gccccggcca
     3241 ccccgccgga actaaacgcc gcgtgaagga gggccatgaa gcgccgcccc gaggtcttct
     3301 ccaggtccac cgctgcagcg ttgatcccca cgtcgtagag cccggtggcc gagtagagta
     3361 cgatcagcgc cgccagcaac atggcgtatc cattggcgaa ggcgacccct atgatgccga
     3421 gcccgaagac cagagagacg gcgacgaggt acgccctgcg gcccagccgg tcggcggtcc
     3481 atccgagagc ggccatcgcg agtatggaag ccgccgcccc ggcgaacagc gcgactccca
     3541 gaggaccggg cgagagatcc agcgcccggc tcaggtccgc gagcaggacc gcaaagcatc
     3601 ccgcccagag accgaagaag ccgaaagctc ccagctcgac agccaggagc ctccagatgc
     3661 gcgggccggg gctactctgt gtcgtagcct cgctcctgcc aggcgtttac cccgccctcc
     3721 atgttggcga cgttcgtgta gcccatcttc tgcaaggact tcgccgccag agagccccgt
     3781 cctccgaggg cgcagtggac cacgatcctg gcgtccttct ccgggagctg agcttcggcc
     3841 tggagctcca gaatcccgcg tgggacgtgc cgcgcgccag ggatgtgccc ttcctcccac
     3901 tcatcgggct ctcgcacgtc cacgatcgtc acgctctccc cggaatccag ggcgtcgcgg
     3961 acatcctcga cgcttacctg ctctgtctca gccttcgctt cggagactat ctcctcgtag
     4021 ctcttcgcca cattcctcct ttccgtcggg atcagccagc ctatgccttg cggtgctcgg
     4081 tcgccaccgt aacggtgccg tcgggcaact ccccgaaagt gtagtcgccg aagaggatcg
     4141 ggtcggcggt ccgcaggcac aggaagaccc gcaccgcgag gttccggatc tcgctctcgg
     4201 cgtgcgcatc ggcccgcatc tcgatgatgt gcctcaaggc ccgcacgttc cccgtgaaca
     4261 ccatcggcgc ctcggtctcg ttgggcagga gactgcgcgc cgtctgctgc accttcttcc
     4321 tggcatccgt cttgtagtcg gccgagagca tggcgctccc accctcctgg tactccaaca
     4381 gcctccccgc catcgcctcg tactcggccg cggcccggtc cgcccgctcc tcgaagagcc
     4441 ggtgcaactc ctcatcctcc tggtactccg gacgctcgac aaagcgcagc acggagccgc
     4501 taacgtagcg ctgagaaatt tgggagaatc ccgctccggc ccggtgacgc accagctcgt
     4561 gcgtgacgct gcggcttatg ccgtagagca ggaagctgaa gctcgcgtgc tccagcaccg
     4621 aaccgtgccc cgccgaggtc aaccgctcga agtaagcggc ggcgttctcg ttagtagtgc
     4681 gccggggacc gaacgatgcg taacagttgc cggagaagtg aggtttgcca tcacgacgaa
     4741 caaatacgat cccgttctcg gtggtgacac agtagacttt gccgctgtag gtctttctct
     4801 cgactctcgc gttcttgttt gcccacgctt cggtgcgagt cctcttggat acgatgtaca
     4861 cctggagtct tccttcgggc gtcttcttgt gcatcagcga agaactaaag cccagcttgc
     4921 agaggatctc ttgaacctgc ccagcgaact ccagggaggt cgtgacgaaa ttctccctcc
     4981 cactttcgcc ccgatggccg tcggtcgcca cgaggatctc ccagacctcc gttagcaggt
     5041 tcgtggggag atccaggacc caacgcggca acctcttgcg ggcgcttccc acgccgcact
     5101 cctgctcgaa gaaagtccgg atcgcccgtc ctccacccac cagcgtgcgg gggacgtcgt
     5161 ttctggggtc tagccaccgg ctcgacttca ggcctagagt ctccgcgagg gacgaaacct
     5221 tctcgcaatg gtcaccgtag accacgactc cctcgccccg ccccacaatg gaagaccctt
     5281 ctgctgcgta gtagacgcac agcaaggcca acgcccggat ctcctcgacg ctctcgacca
     5341 cgcgctcgga cacgatggtt gagttgctgc cgtaggaacc gtactggttg cagattcgct
     5401 gaccgaactc gaaccctggg atacgaaccc gatccggtat ctcccccacc caccccgaag
     5461 aggtggtgcg aaccgcgaaa cgtctgctcc atatctcctt ggtagagtag aactcgtacc
     5521 cgccgtcctt caggcgtgcc cactggcgat gatcgggggt taccgcgaaa gacagagcct
     5581 tgccttcgca gacaaacaag tcatcgtcat gatcgtaaac ctgatacgcg agcggctgct
     5641 ggaactcggc ttcgcctgta tcgggattta gcgtgagtac ccgctcaccc tggtgcaggt
     5701 tctgaaattt cttccaccct tcgtccgtga gtatctctgt ttcgtcgtcg aagcagagct
     5761 ggcccgccgt cttgcagagc tgagaagagc cggggagcgt ggtcggatct tccaggtagc
     5821 ctgggaaccc aagatccggg tcgaagcctt cgaggaaacc ggcgaggccc gctacgttga
     5881 cctgcggctt cgccagcatg acgacgcccg gcctcctcag gtacgcggtc ccggcgccgg
     5941 tatggctgac gggggagtga atggccggga agccgtcatt cacgctccct cccaggctct
     6001 cgaacaactc ctgcacgcgc tccgcgctac gcccggttgt ctccaaaagg ctcccttctc
     6061 cggtgtgttg cagagaaggt tagcctattt gacggggttc tacacggcct cggagagggc
     6121 gcgccgcagg gtgctggagt agcgtccggt gcggatggtc cttagggctt tgatctcgac
     6181 ctgccgcgcc cgctcctgcg agatgccgag cacctcggag acctcgcgca gcgtgcggat
     6241 cctgccgtcg tccaacccgt gtcgcatctg caataccttc gcctcgcgct ccggcagacc
     6301 tcttacggcc tccacgagcg ccccttccca gctcccgatc tcgacgcgct cgtagtcctc
     6361 gccggaacgc tcgtcggcga gcaactcccc catctcggag ccgtcgtcgg ccccggtgcc
     6421 catccgggcg ttgatgctgg acaccggctg gctcacctcg cgcagcctgc gcgcttcttc
     6481 aggcttcaca tcgagcctgg ccgccaactc ctcctcggtg gcgtcgcggc ccaattctac
     6541 ggagagcgcg ttttcggcgc ggcgcaggcg gaacacggcg tcaactacgt gagcggggag
     6601 gcggacggcg cgtccgtggt ccgcgatagt gcgggtgacg gcctgacgga tccaccacgt
     6661 agcgtaggtc gagaagcggt tccccatctc cgggtcaaac ttctcgaccg cccggatgag
     6721 ccccgcgttg ccctcctgga tgaggtcctc gaacgccacg ccacgccccc ggtacttctt
     6781 ggcgatggag attacgagcc gcaggttgca ctcgatgagc atcctgcgcg cccgcatgtc
     6841 tccctcgcgc acccggcgcg acagctcgat ctcttcccgg cggtcgagca gccgcccgtc
     6901 tcttatctgt cccaggtact gcgtcagaga gtcttgcgcc tgcatcatcc cacgtcccct
     6961 tccttagaac ctatggtaga acctacgccg ccgagggccc atctccgaaa ccgccgttca
     7021 cgcgcggacc gccgtgcgtc acgaaacgct ccagcacccg ctcatctaag ctggaggtga
     7081 tccaccactc ccgcccgcac gagcacgcca acgagagcga gcgtctgatc cccggctccc
     7141 ggagcgccgc ctccttgagt gaccggatgc agcacgtcgg gatctcccgc acgtatacct
     7201 cccggtgcgg ctctccagca ggagcgaccg tcgtatccgg ccgcagagcg ctcctcttcc
     7261 tcacgccgct cctctcctct catcctccga accgcgccgc atgaacgcat cctcttccgg
     7321 gaccacaggt atcggggcct gccacatcgc gccgcaggag tggcactcga agccatcttc
     7381 cgcgatccgg gcgcttctct ggcagcaggc ggggaggtcg gcctcgtaga ccgactcctc
     7441 gcccatagcc gcgatccaga gccgctcgct ccgcgcttcg ctcctcactg ctacgccgcc
     7501 ttcccggaga ggcgcccgat ccactcactc gcctcctcgc gggtgagctg gcccaccggc
     7561 ttcccgacca tgtcctcgaa cctggccagc ccatcctcga ccacgtccgc gatgagggcc
     7621 tccaggtagt tgagctgctt gcgcgtggcc ggcaactcgc ccttgccttc ctcccgaggc
     7681 gcccgaacct gtttctccgt ccgctgcccg tcgccggacg cgacgccggt ctcctcagcc
     7741 ggcacggcgc tctcctcgcc gccgagctcc tcgaaggcgg tgacgccgac gttgatcgca
     7801 tcgcgcagcg cccgcgcctt cgcccgtgtc tcggccatcc gaatcaggtg cggcgctatg
     7861 gcccggttga cgttctccgg gctcgcgtcc ccgatcccgg tgtacttgcc ctcttccgtc
     7921 cggacggtgg ccttcactat ggcgacctcg ccgttctccg gggccggtat ctgcaagaga
     7981 tccgtctcga tgctcctgag tccccgcgcg tgcgcctcct ccagaagccc cgcgtacaac
     8041 acgaaccgct tgccctgccg ctcgatcatg tactcttccc gcatcgctat acctccttcg
     8101 gcgctcccgc accgatattg ctgtcttcac tgaattgagt gaggatgtgg tcaaataaaa
     8161 acatcaggcg atccgctcgt aggtgtactg gcgtccgccg ggagcggcgg tctcgacttt
     8221 acggtggatc agaccgagct tcgcgagcct cgtcgcccgg ttgttgcagg cggtgtggtt
     8281 gacgccgagc ttcttggcga gatccgcgtt ggtgatggat ccggcggccc gtatctcctc
     8341 cagcgtctcc accaggtgat ccggcaccgt cccgacgatg cccggctccc cgccgacctc
     8401 gtccacccgg atcatcgcga gcgaccgctg ccgcagggac gcctcgatgt tctccagcat
     8461 gtcccggtcc tcctcgacca gcaccagccg ccggtccccg tactccccgc ccgcgaccct
     8521 cgacaccaga acccccagcg cctcgtccgc gaacgagtag tccatcaact cgacgcctcg
     8581 cgtgtccaca tacagggtgc cgcccttcgg caacccctcc agcgcctcgc ccagcgcctc
     8641 ccggaccgcc cggcccgtcg agcgggtgac catcagctta cccccgaagt cgcctcccgc
     8701 gacctcgtag accaagcgtg agtggtcctc agtcaccggc gctgtcctca ctgtcttcag
     8761 tgaaatcata taagtgaagt gtactctctg agcgctggaa tatcaatggc atatggagcg
     8821 gtattttgcg ctcatcggga acttttttca cgcggatcgt gggcgaggcg cgggagggag
     8881 acgcggacga aggtgccggg gaagaacggg acgttgcggg agttcttgcg gtcctcgtag
     8941 acggttacgc ggccggtgcc ggagcgcaag gagagggagc ctcccgctcg gccggagatc
     9001 tgggctacga gcgccaggcc gccgccgcgc ccgagctcgc cggtgccgga gacgcccatc
     9061 atcatggcgt ggcgcagggc gtcgttgtcg gtggtggtgt gctcggcgta cttcgggttg
     9121 cgggagaggg tctctcgcac gcccggcccg ccgtcggcga tggcgatgag cacctcctcc
     9181 ccgcgccggc ccgtgccgct gacgacgtgg tggtaggcct ggacggcggc gtaggccccg
     9241 aaggaggagc ggccatgctc ggcggcgttc gcgcagatct ccgagaggac cgagcagatg
     9301 gcgacccgct cgcgcacccg gtagccctga ttctccagga tctccgagat gcggtggatg
     9361 atgccgggga tctcctcctc ggtgtggaag tttattagct cctgcaaggt gccggggttg
     9421 tgccgctgcc gctcctccag ggaggccagg tccgcgttag tcttcacccg cccgccgaag
     9481 tgccggaaga agtccatccg ctccaggtag gccgggacgt cgcggcccga gagcgccagg
     9541 tcaacc
//
LOCUS       DASSMB010000061        14767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_646734, whole genome shotgun sequence.
ACCESSION   DASSMB010000061 DASSMB010000000
VERSION     DASSMB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14767
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646734"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="VFJ72_04245"
     CDS             <1..106
                     /locus_tag="VFJ72_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7478711.1"
                     /translation="EPEDLKGAVVFLASDAASYVHGAILPVDGGWLGR"
     gene            84..1124
                     /locus_tag="VFJ72_04250"
     CDS             84..1124
                     /locus_tag="VFJ72_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET7478712.1"
                     /translation="MAVGWDANACIETPAFFGVRRRYNVRDVMESGIGAGLGRVVAVR
                     ADVSEALERWSAVVALESTLISHGLPRPDNLELAREVEEIVRAEGAVPATVGVIGGVP
                     KVGLDADELELMAEEDGILKLSARDLAFAVAAGSHGATTVAATAHLAALAGVEVFATG
                     GLGGVHREARESWDVSADLMTLARTPVAVVCSGVKSILDVPATLEALETFGVPVAGFR
                     TRRFPGFYLTDSGSPLDWSLDSEEEAARAILALHEVGPEGSGMLIANPIPEEEQLDPA
                     LHDRVLDAALAELKRREIRGKEVTPFLLDQFQRTTAGESLKVNKQIIRNNASLAARIS
                     VRLANLRAANRT"
     gene            1128..2042
                     /locus_tag="VFJ72_04255"
     CDS             1128..2042
                     /locus_tag="VFJ72_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET7478713.1"
                     /translation="MRASRERASPRASRVLVVGDLLYDLLAEVEDVAFGTDTFAPIHA
                     AAGGSGANAASWLARCGVETHFAGRVGDDVFGGFLAGELERAGVIPHLARDRDLRTGK
                     VFVLVGAEGERTMITDRGASENLRPEDLPTSLFQRGTHLHLSGYTFSAGTRRETAFGA
                     LRLARDSSMTVSIDPSSVPLLQDIGPERFLEWTAGADLCLPNLEEGALLAGTREPEAI
                     VFALCEHYRGVVLKLGAGGAIYAGEDAELVRLPAMAVRALDTTGAGDALCAGFLSGWV
                     TGSSPEDALRRGVEISGGVVSRIGGRAE"
     gene            complement(2050..3189)
                     /locus_tag="VFJ72_04260"
     CDS             complement(2050..3189)
                     /locus_tag="VFJ72_04260"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HET7478714.1"
                     /translation="MRTRWRDILRKRLLYLIEAGEHRFDRMKLYLKVRHGRLGPVYIL
                     PYLGHGTARAVHITGRVMEEKKVYLPAVDDSAWRNLRAMIRRFLSAEVPGARVRVRFA
                     GRDRLVVADDEGFFQARMEPPEPLPEDNAWREIGLELMWPLAKGQREAQAVGEVMVPP
                     EDVEFGVISDIDDTVVLTGATSAFRTLRLLLFSNAHTRLPLEGVAPFYRALRAGPDGG
                     AGENPIFYVSTSPWNLYDLISDFLDLRNIPAGPIFLKDWGGVKDALRGMDHRAHKLLV
                     IRELLDTYPGLPFVLVGDSGQQDAETYAQVARENPGRIRAIYIRDIERPITGRDLAVR
                     EISAEVEAIGVPMLLVEDTVTAANHAADHGLIDPASLPEIRAAEK"
     gene            complement(3192..4574)
                     /locus_tag="VFJ72_04265"
     CDS             complement(3192..4574)
                     /locus_tag="VFJ72_04265"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7478715.1"
                     /translation="MRTRGILAKVAGITGTVLLGSLLAAGPRQEPRRSAEGPMRDAFE
                     SAAVGLALLDADGCVAEANASLRNMLGYDAREIRGLSLNAAFRADGGVREGLLSSMLA
                     GDGHYRGEGRLGRKDGGDVLVKIEVSPLWEDGGRGVAVIEDITRARRVEEAFAESEVR
                     FRQLFENSVDALLMHDEEGRILDCNVEACRSLGYTRAELLSLSVRDIAVDVMSEEQQE
                     AVGEEAAWRRAMRGRPGAVVGTYENRHRRKDGTTFPVEVALASMDYEGRRVIFAAARD
                     ISERKALEERLTYQAFHDSLTGLPNRALFLDRLEQALARADRREFSIAVFFLDLDNFK
                     DINDTFGHEVGDRLLAEVGSRLTSCVRAADTVARLAGDEFTVLLEELGNGDEAVRVVE
                     RIETIFQEPVTVNGRDLSVTASIGVVVDAQSGRSPRELLNDADIAMYRAKRAGKARYE
                     IHVPGDSGSD"
     gene            complement(4616..5338)
                     /locus_tag="VFJ72_04270"
     CDS             complement(4616..5338)
                     /locus_tag="VFJ72_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7478716.1"
                     /translation="MLARLDHLVLLVRDLGEAALEHERLGFTVTPGGEHADGLTRNAL
                     IPFADGSYVELIAFLDPEDPTDNVWNWRPFAALGGGLIDYCAASDDLDGDVRRLRAEG
                     FEVDDPTSGGRTLPGGEEILWRVARIRQEGRVLPFLIEDQTPRGLRVPSGPAAAHPNG
                     ARGISRLEIAAPDAEEAADSYASLTGASHENGLRIGSCAVEIVDLKDGSRAGNRQRSP
                     RAGPLSVILDAGGTKVIRLPAR"
     gene            5433..5858
                     /locus_tag="VFJ72_04275"
     CDS             5433..5858
                     /locus_tag="VFJ72_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478717.1"
                     /translation="MSDTEGSYLSQLDEETMRRAWGERTPEDRRRIITAAMIFGRQLD
                     ARLAQTTASPALEDTQRFLMSLMNAVVDEFAEREEIDRNEAASFLGDVTNRDHILEFN
                     EVIEDYETGDSNKSLDALLREAIRDRREKAIWSGHWSSG"
     gene            complement(5887..6030)
                     /locus_tag="VFJ72_04280"
     CDS             complement(5887..6030)
                     /locus_tag="VFJ72_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478718.1"
                     /translation="MSEVWQFVGVLGGFVVVALLWLNIVYLPGRTLERWEDRRPGRGP
                     RDD"
     gene            6228..6872
                     /locus_tag="VFJ72_04285"
     CDS             6228..6872
                     /locus_tag="VFJ72_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET7478719.1"
                     /translation="MSERYTTSEEVANGVTHGLGLAASVAGAAALVLLTAPGGDAWRI
                     VSSVVYGVTLISLYTASTLYHLSRRPGAKRVLRVLDHCAIFLLIAGTYTPFMLVSLRG
                     GWGWTLLALVWSLAAAGIVFKVFFTGRLDALSTSVYILMGWLCVVAAKPMLDMVPAGT
                     LLWLLAGGLVYTAGTVFYHNRRVPYSHAVWHLFVIGGSLCHYLAVSSAVLPPIT"
     gene            complement(6894..7073)
                     /locus_tag="VFJ72_04290"
     CDS             complement(6894..7073)
                     /locus_tag="VFJ72_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478720.1"
                     /translation="MPTDDNAASLEWAESSLEYARSCGPGRLVELLVLVLDEILFEMG
                     PAGDSATHGRESRGR"
     gene            7274..8008
                     /locus_tag="VFJ72_04295"
     CDS             7274..8008
                     /locus_tag="VFJ72_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7478721.1"
                     /translation="MDDGKLKRGSAEEDIRLLSMVDIFEPLSREEIEAINWRRLETSV
                     GRGETFYMPMDLGETLFILKSGRVRLFKKSADGREFTLAVLESGTVFGEMSITAQRLR
                     NAYAQAIEPSRIAAMCRADVERLIMEKPRVGLQLIHLLSDRLNAYEIRMEDLAFKKVP
                     ARLASLIFLLIESQGVRTSTGYKIPTRYTHQQLGTMIGANREAVTRAFSILREAGAVE
                     VRRRYICVENLEVLQKAAESNSYQEA"
     gene            8081..8656
                     /locus_tag="VFJ72_04300"
     CDS             8081..8656
                     /locus_tag="VFJ72_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478722.1"
                     /translation="METFLLRQFQREIERQCQFAMIALQDIEESSANSDGKLFWYSIQ
                     NLLVAIGRISRLLWPPDESASERGAELRASLGVGDDSPLKSRTYVEHFENFDERLMDW
                     YETSEHHRYFDSYTEPLDILAETKGGDRFRGYDTEKNAILFHSEPYYLGPISEAVEVL
                     CQRAEQEMQKPRFDLDPEKPSARDEEDIVRY"
     gene            9048..9953
                     /locus_tag="VFJ72_04305"
     CDS             9048..9953
                     /locus_tag="VFJ72_04305"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC and DUF2382 domain-containing protein"
                     /protein_id="HET7478723.1"
                     /translation="MAEFEERSDRFTAVEDRFAGYTVYDRDNDKIGKVDDLFLDENDQ
                     PEYFGVKMGFLGTRSTLIPAEIAQIDESQQAVLVSSEKQYVKDGPSFDDDREITPDFE
                     REVRGYYGVDGQSPDTERGSYGSYYSDSDQEFGAETDRDSGEHHPGVAMGDREGGEFR
                     EHGVHEEGATEPGGDLDDQDELRVQRTEEELRAGTTEREAGRMNVRKRVRTDRESIGV
                     PTKREEVHVERVPVDREAGETEIGDDEISVPITEEEVVVEKRPVVKEEIRLRKDVVED
                     EQVVEEDVRREEVDIDDATERRRDV"
     gene            10143..10793
                     /locus_tag="VFJ72_04310"
     CDS             10143..10793
                     /locus_tag="VFJ72_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478724.1"
                     /translation="MKDSEQRGNRDAPRVEENETRSEDRRGESAPRRVGDVRRDHEAE
                     RHRRESELGTSWTSVTFGWLTALGAGLILSGIVGGIVGAILGAMGVKGGTEGGVAALV
                     GLLITLLLAFLIGGYVAGRMASRAGVKHGLLVPLLALVVTLLLAAIGTAIGYSFIDQL
                     SGVTLPKIPGGVQNQIPQQNLATLLTVPGILALLFPFIGGAIGGAWGAKTGRNRPY"
     gene            10857..11594
                     /locus_tag="VFJ72_04315"
     CDS             10857..11594
                     /locus_tag="VFJ72_04315"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478725.1"
                     /translation="MNILQSLQQGVSAFFAFIPQLIGAIVILIVGYIVAKVLEAVVGR
                     VLKAIGFDGWMEKSGIKQFFDRAQTRQTPATILGKLVFWFVFIIAITMATDALGISQV
                     SAVFSQLIAYIPNIIAAVLILILAGLLAQFVAGLVRGATGVDILGTVAQVAIMVYAVF
                     AALTQLGIAVQLTAPTFLIVLGAVALAAAIAFGWGSRNVAQDIVEKAYARRGEAQEKI
                     QRERQRSDSDSDADIGTGRPSVHRREV"
     gene            complement(11673..12323)
                     /locus_tag="VFJ72_04320"
     CDS             complement(11673..12323)
                     /locus_tag="VFJ72_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET7478726.1"
                     /translation="MCGRYTLKTPLDTLVKHFGLTEYPPRLSSSYNIAPTQLVAAVTG
                     SGRLETLRWGLIPSWARDDSIGARLINARAETVNEKPSFRSAFRRRRCLIPADGFYEW
                     RRSNGGKQPYYITVRGGEPFAFAGIRESWSGPEEEVHSCAIITTRANPLVAGIHDRMP
                     VILPPDAYDPWLDPEAEAQEAISLLEPFSPDEMDAYPVSARVNRPANNDERCVQPL"
     gene            12436..12861
                     /locus_tag="VFJ72_04325"
     CDS             12436..12861
                     /locus_tag="VFJ72_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478727.1"
                     /translation="MISWFRRRGNGIRLELGQSLGAFTSPDGDISPLFVFFEVANAGG
                     EDVTLAAVRVTPRGIPHTVLADAAKGTLEGGHDLPRTLAPGESIRFQVRAKTLAGRAR
                     DAGHSGRARLEFAAEDTSGNRHRKVFRFRVDEYLRLKDE"
     gene            complement(12909..13301)
                     /locus_tag="VFJ72_04330"
     CDS             complement(12909..13301)
                     /locus_tag="VFJ72_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478728.1"
                     /translation="MASMKVLIAIEPRSYREVIGLAIEELRPHLRVEILEPDLLESEV
                     SRLEPDLVFCSRSDCLPGSCERAWIEFRPYAETQATVCLGGVISTMEGVDLDDILSIV
                     DRAERLLRGPLPSTCEPGARRQLRSTRL"
     gene            complement(13480..14187)
                     /locus_tag="VFJ72_04335"
     CDS             complement(13480..14187)
                     /locus_tag="VFJ72_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7478729.1"
                     /translation="MTQPIDLYYWPTPNGHKVSIFLEEAGLPYNVKPINIAAGDQFEP
                     EFLKISPNNKMPAIVDPEGPDGEPISLFESGAILIYLAEKTGRFFPQSPRDRYLVLQW
                     LMFQMGGVGPMLGQTHHFRNYAPDEIPYAIERYTNEAARLYGVMDERLSEAEFFAGGE
                     YTIADMAVYPWVVPYENQGQDMDDFPNLKRWFEAVGSRPAVRRALDVGKELRRKLDRD
                     MDDDTRETLFGNRRRKV"
     gene            complement(14205..14519)
                     /locus_tag="VFJ72_04340"
     CDS             complement(14205..14519)
                     /locus_tag="VFJ72_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HET7478730.1"
                     /translation="MIIAEVGIIPIGTETPSVSRYIAAAVKELEVSGLKCSLSALGTT
                     VEAESAEQFYAALGRAQEAVFGLGIGRVYTIVKMDERRDVEGRSGDDMVRSARQQAGE
                     SE"
BASE COUNT         2590 a         4981 c         4702 g         2494 t
ORIGIN      
        1 cgaacccgag gacctcaagg gagcggtggt cttcctcgcc tcggacgccg cctcctacgt
       61 ccacggcgcg atcctgccag tagatggcgg ttggctggga cgctaacgcc tgcatcgaaa
      121 ccccggcgtt cttcggtgtc cggcgccgct acaatgttcg ggacgtgatg gagtccggga
      181 ttggagcggg tttgggtaga gtcgtggcgg ttcgagccga cgtatcggag gcgctggagc
      241 gctggagcgc ggtggtggcg ctggagtcca ccctgatctc tcacggcctc ccccgcccgg
      301 ataacctgga gcttgcccgc gaggtggaag agatagtacg cgcggagggc gcggtcccgg
      361 ccaccgtcgg cgtgatcggc ggcgtaccga aagtcggact ggacgcggac gagctggagc
      421 tgatggccga agaagatggg atcctgaagc tctccgcccg cgacctggcg ttcgccgtcg
      481 ccgctgggag ccacggcgcc acgaccgtag ccgccaccgc gcacctcgca gcgctcgccg
      541 gggtcgaggt cttcgccacc ggcggcctcg gcggcgtcca ccgcgaggcc cgcgagagct
      601 gggacgtctc ggccgacctg atgaccctcg cgcggacgcc cgtggccgtc gtctgctccg
      661 gcgtcaagtc catcctcgac gtgccggcga ccctggaggc gctggagacc ttcggcgtcc
      721 cggtggccgg gttccgcacc cgccgcttcc ccggcttcta cctaaccgac tccggcagcc
      781 cgctcgactg gtccctggac agcgaggaag aggcggcgcg cgccatcctg gcgctccacg
      841 aagtcgggcc ggagggctcc ggcatgctca tcgccaaccc catccccgaa gaagagcaac
      901 tcgaccccgc gctccacgac cgggtcctcg acgcggccct cgcggagttg aagcgacgcg
      961 agatccgggg caaggaagta acgccgttcc tcctggacca gttccagcgg acgacggcgg
     1021 gagagagcct gaaggtcaac aaacagatca tccgcaacaa cgccagccta gcggcccgca
     1081 tctccgtccg actggcgaat ctccgcgccg cgaaccggac ctgagcgatg cgggcatccc
     1141 gcgaacgcgc ctcgcctcgc gcctcccgcg tcctggtagt cggggacctg ctctacgacc
     1201 tcctggccga ggtggaagac gtggccttcg gcacggacac cttcgcgccc atccacgccg
     1261 cggcgggcgg gtccggcgcc aacgccgcct cgtggctcgc acgctgcggg gtcgagacgc
     1321 acttcgccgg ccgggtcggg gacgacgtgt tcggcggctt cctcgctggt gagctggaac
     1381 gcgcgggtgt cattccgcac ctcgcccgcg accgggacct gcgtaccggc aaggtcttcg
     1441 tgctcgtggg cgcggaaggc gagcggacca tgatcacgga ccgcggcgcc agcgagaacc
     1501 tgcgcccgga agacctgccc accagcctct tccagagagg cacgcacctc cacctctccg
     1561 gctacacctt ctcggccggc acccgccgcg agaccgcttt cggcgcttta cgcctcgccc
     1621 gcgactcatc catgaccgtc tccattgacc cctcttcggt gccgctgctc caggatatcg
     1681 gcccggagcg cttcctggag tggacggcgg gcgccgacct ctgcctcccg aacctggagg
     1741 aaggagccct gctcgccggc acgcgggagc cggaggccat cgtctttgcc ctgtgcgagc
     1801 actaccgcgg cgtcgtgctc aagctcgggg ccgggggcgc tatctacgct ggtgaggatg
     1861 cggagctggt gcgtctcccg gcgatggcgg tgcgggcctt ggatacgacg ggcgcgggtg
     1921 acgccttgtg cgccggtttc ttatcgggct gggtgacggg ttcttcgccg gaagatgcgc
     1981 tgcggcgggg ggtcgagatc tcgggcgggg tagtaagcag gatcggcggt cgggcggagt
     2041 aaacggcggc tacttctccg cggcccggat ctccggcagg ctcgcggggt ctatgagacc
     2101 gtggtccgcg gcgtggttgg cggcggtaac ggtatcttcg acgagcagca tcggcacgcc
     2161 gatggcctcg acttcggcgg agatctcacg caccgccagg tcgcgtccgg ttatgggacg
     2221 ctctatgtcg cggatgtaga tcgcgcgtat gcgtccgggg ttctcccgcg cgacctgggc
     2281 gtaggtctcc gcgtcctgct gtccagagtc cccgaccagc acgaacggca ggccgggata
     2341 tgtgtcgagc aactcgcgga tcaccagtag cttgtgggcg cgatggtcca tgccccgcag
     2401 cgcgtccttc acgccgcccc agtccttcag gaagatggga ccggcgggga tattccgcag
     2461 gtcgaggaag tccgagatca gatcgtacag gttccacggg ctggtagaga cgtagaagat
     2521 ggggttctcc cccgcgccgc cgtccggccc ggcccgcagc gcccggtaga agggcgcgac
     2581 accctccagc ggcagccggg tgtgcgcgtt ggagaagagg agcagacgta gcgtgcggaa
     2641 ggcgctcgtc gccccggtca ggaccacggt atcgtctatg tcgctgatga cgccgaactc
     2701 cacatcctcg ggcggcacca tcacctctcc cacggcctgg gcctccctct ggcccttcgc
     2761 cagcggccac atcagctcca ggccaatctc ccgccaggca ttatcttccg gcagcggctc
     2821 cggcggctcc atgcgagcct ggaagaaccc ctcgtcgtcg gcgactacga ggcggtcgcg
     2881 gcccgcgaag cgcaccctca cgcgggcgcc cggcacctcg gcgcttagga agcgccggat
     2941 catggcccgc aggttgcgcc acgccgagtc gtccaccgcc gggaggtaca ccttcttctc
     3001 ctccatcacc cggcccgtga tgtggaccgc ccgcgccgtg ccgtgcccga ggtagggaag
     3061 tatgtagacc ggcccgagcc gcccgtggcg caccttcagg tagagcttca tccggtcgaa
     3121 gcggtgttcg ccggcttcta tcaggtacag gagcctcttg cggagaatat cgcgccagcg
     3181 cgtccgcacc tctagtcgct cccggagtcg ccggggacgt ggatctcgta gcgggccttg
     3241 cccgctctct tcgcccggta catcgctatg tccgcgtcgt tcagcagctc ccgcgggctc
     3301 cgcccggact gcgcgtccac cacgacgccg atgctcgccg tcacggagag gtcccgcccg
     3361 tttaccgtaa cgggctcctg gaatatcgtc tcgatcctct cgaccacccg caccgcctcg
     3421 tcgccgttcc ccagttcctc cagcagcacg gtgaactcgt ccccggcaag acgggcgacg
     3481 gtgtcggctg cccggacgca cgaggtgaga cggctcccga cctccgccag cagccggtcc
     3541 ccgacctcgt gcccgaaggt atcgttgatg tccttaaagt tgtccaggtc caggaagaag
     3601 acagctatgc tgaactccct ccggtcggcg cgcgccagcg cctgctccag ccggtccaga
     3661 aagagcgccc ggttcggaag ccccgtgagt gaatcatgga aagcctgata cgtgagcctc
     3721 tcctccagag ccttgcgctc cgaaatatcc ctcgccgcgg cgaagatcac gcgccgcccc
     3781 tcgtagtcca tggaggcgag cgccacctcg accgggaagg tcgtgccgtc cttgcgccgg
     3841 tgcctgttct cgtacgtgcc cacgacggct ccgggccgtc cccgcatcgc ccgccgccag
     3901 gccgcctctt cccctaccgc ctcctgctgc tcctcggaca tgacatccac ggcgatatcc
     3961 cgcaccgaga gcgagagcaa ctccgcccgc gtgtaaccca gggaccggca cgcctcgacg
     4021 ttgcagtcca ggatcctgcc ttcctcgtcg tgcatcagga gagcgtccac ggagttctcg
     4081 aagagttgcc tgaaccggac ctcgctctcg gcgaacgcct cctcgactct ccgcgcgcgc
     4141 gttatgtcct cgatcacggc cactccccta ccgccatcct cccacagcgg cgatacctcg
     4201 atcttcacca ggacatcccc tccgtctttc ctgccgagac gcccttctcc ccggtaatgt
     4261 ccatctcccg ccagcatgga agataacagc ccctccctta caccgccatc ggcgcggaaa
     4321 gcggcattca gggagagccc gcggatctcc cgggcatcgt agccgagcat gttccgtaga
     4381 gaagcgtttg cctcggcaac acatccgtcc gcatccagga gcgcgagccc cacggcggcg
     4441 ctctcgaacg catcccgcat cgggccttcc gcgctccggc gcggctcctg acgcgggccc
     4501 gcagccagca ggcttccaag aagaaccgtg ccggtaatac ccgcaacctt tgccaggatg
     4561 cctctcgttc gcaagactat cactctccgt cagactacca ggaactctcc gggtattatc
     4621 gtgccggtag ccggataact ttagtccctc cagcatcgag aatcaccgag agcggtccag
     4681 cccgtggact tcgctggcgg ttcccggccc gggatccatc cttgaggtcc acaatctcca
     4741 cggcgcaaga ccctatgcgc agcccgtttt cgtgtgaggc tcccgtcaga gaggcgtaag
     4801 aatccgcggc ctcctccgcg tcgggagccg caatctctag ccgcgagatg ccgcgcgcgc
     4861 cgttcggatg ggcggcggcc ggtccgctgg gcacgcgcag gccgcggggg gtctggtctt
     4921 cgatcaggaa cggcagcacc cggccttcct gccggatgcg ggcgacgcgc cagaggatct
     4981 cctcgccgcc ggggagtgtc ctgccgccgc tcgttgggtc atccacctcg aacccttccg
     5041 cccgcaagcg gcgcacgtcg ccgtcgaggt cgtcggaggc tgcacagtag tctatgaggc
     5101 caccgccgag cgccgcgaac ggccgccagt tccacacgtt atcagtgggg tcctccgggt
     5161 cgaggaaggc gatcagctcc acgtatgagc cgtcggcgaa cgggatcagg gcgttgcgcg
     5221 tcaggccgtc ggcgtgctcg ccgcccggcg tgaccgtgaa gcctagccgt tcgtgttcca
     5281 gcgcagcctc tccgaggtcc cgcaccagca acaccaggtg atccaaccgg gccaacacag
     5341 gcttctacct cccaggggtc ctcgccgcct ctgataccat actcgcataa cgacgtcccg
     5401 gccaatagac gatgaacgag gacgaacccc gcatgagcga caccgaggga tcctacctct
     5461 cccaactcga cgaggagacc atgcgccgcg cctggggcga gcggacgccc gaagaccggc
     5521 gcaggatcat caccgcagcc atgatcttcg gccgccagct agatgcccgc ctggcccaga
     5581 ccacagcttc tcccgccctg gaagacaccc agcggttcct catgtccctg atgaacgccg
     5641 tagttgatga gttcgccgaa cgcgaggaga tagaccgcaa cgaggccgcc tccttcctcg
     5701 gcgatgtgac caatagggat cacatcctgg agttcaacga ggtgatcgag gactacgaga
     5761 cgggagactc gaacaagtcc ctggacgcct tgttacgaga agccatcagg gaccggcggg
     5821 agaaggctat ctggtccggc cactggagct cgggctaaag agcccgagcc acgcgcggct
     5881 cacttactaa tcgtcgcgag gtccgcgtcc cggcctccgg tcttcccacc gctccagggt
     5941 tcgccccggc aggtagacga tattgagcca gagcagcgcg actaccacga accccccgag
     6001 caccccgacg aactgccaga cctccgacat caaaatctac tccctctcgt ccgtttcgcg
     6061 gactcgcttc gtactccgtt agtatctgcc gacatagtat cgtctaccgg ggaccgcggc
     6121 gccgttaacc gtttaaccgg ggccgcgcga atactctggc ggacgccgtc ttactcggat
     6181 agagttgagg aggtccaacg aaagcgaggc gtctgccgga tggcccgatg agcgagcgtt
     6241 acacgacttc ggaagaggtg gcgaacggcg tgacgcacgg cctcgggctg gcggcgagcg
     6301 tcgcgggagc cgccgcgctg gtactcctca ccgcgcccgg cggcgacgcc tggcgcatcg
     6361 tcagctccgt cgtgtacggg gtcaccctga tctcccttta cacagcctcg acgctctacc
     6421 acctgtcgcg gcggcccgga gcgaagcggg tcctgcgggt cctggaccac tgcgccatct
     6481 tccttctcat cgccgggacg tatacgccgt tcatgctggt cagcctgcgc ggaggctggg
     6541 gatggacgct gctcgcactc gtgtggagcc tggcggcggc cggcatcgtc ttcaaggtgt
     6601 tcttcaccgg acggctcgac gccctgtcaa catccgtcta catactgatg ggctggctgt
     6661 gcgtcgtggc ggcgaagccg atgctcgata tggtccccgc cggaacgcta ctctggctcc
     6721 tcgccggcgg gctcgtctac acggccggta cggtcttcta ccacaaccgc cgagtcccat
     6781 actcgcacgc cgtctggcac ctcttcgtca tcggcggcag cctctgtcac tacctcgccg
     6841 tctcaagcgc cgtcctgcca cccataacat gagccgtgat gatacgctgc gcgctaccgt
     6901 ccgcggctct cccggccgtg tgtcgcgctg tcaccggcgg gacccatctc gaacaatatc
     6961 tcgtccagaa ccaacaccag cagctcgacc agccttcccg gtccacagct ccgcgcatac
     7021 tccagcgacg actcggccca ctccagcgat gcggcattgt catcggttgg cattacggct
     7081 cttcgttcct ccctccgttg acaggcacct ctgtgtaccc gccctggcgt gtctataatt
     7141 ctgacagatg cgagggcgca gcaggaccgc attgtgcgcg gagacacaac ggacctgcta
     7201 tcgagcggag actcaagctc tggagggtga aataggagcg agcatcagac ataccagcct
     7261 ggacaccgac agcgtggacg acgggaaact caaaagaggt tccgccgagg aagacataag
     7321 gcttctctcg atggtggaca tcttcgagcc gctctcgcgg gaggagatag aggcgatcaa
     7381 ctggagacgc ctggagacca gcgtcgggcg cggcgagact ttctatatgc cgatggacct
     7441 cggcgagacg ctcttcatcc tcaagtcggg ccgggtgcgg ctgttcaaga agagcgccga
     7501 tgggcgcgag ttcacgctgg ccgtcttgga gagcgggacg gtgttcgggg agatgtcgat
     7561 cacggctcag cgattgcgga acgcctacgc gcaggccata gagccctcgc ggatcgccgc
     7621 gatgtgccgg gccgacgttg aacgccttat catggagaag ccccgcgtgg gccttcaact
     7681 gatacatctg ttgagcgacc ggctgaacgc ctacgagatc cgaatggagg acctcgcctt
     7741 caagaaagtc cccgcccggc ttgcgagcct catcttcctg ctcatcgaga gtcagggcgt
     7801 caggaccagc acgggctaca agatacccac ccgctacacc caccaacagc tagggaccat
     7861 gatcggggcg aaccgcgaag ccgttacccg agccttctcc atactgcggg aggccggtgc
     7921 cgtcgaggtg agacgccgtt atatctgcgt cgagaacctc gaagtcctgc agaaagcggc
     7981 cgaatccaac tcgtaccagg aagcatgagc ctctctcgcc cgccccggtc cgcagcaaga
     8041 catagatcac acttcacacc ttccgagaag gagtaagaac atggagactt tcctgctccg
     8101 gcagttccag cgagagatcg aacgccagtg ccagttcgcg atgatcgcgc tgcaggacat
     8161 agaggaatcg tccgccaaca gcgacggtaa gctcttctgg tactcgatcc agaacctgct
     8221 cgtagccatc ggcaggatct cccgcctcct ctggcccccg gacgagagcg cctcggaacg
     8281 cggcgcggag ctacgcgcga gcctcggcgt cggagacgac tcgcccctga agtcaaggac
     8341 ctacgtcgag cacttcgaga acttcgacga gcgtctaatg gactggtacg agacatctga
     8401 gcaccacagg tacttcgatt catacaccga gcctctggac atactcgccg aaaccaaggg
     8461 cggcgaccgc ttccgcggct acgacaccga gaagaacgcc atcctcttcc acagcgagcc
     8521 ctactacctc gggccgatct ccgaagccgt ggaagtcctc tgccagcgcg cggagcagga
     8581 gatgcagaaa ccccgcttcg acctggaccc ggagaagccc agcgcccgcg acgaggaaga
     8641 tatcgtccgg tactgagcga ggcgcatccg ctggcaaccg tagcccgcgc ccggcgaagc
     8701 cgccccgcgc gaagcctcat ggcaggccgc gcttcgagct tgcgatagaa gtcctcacgg
     8761 aacccggcgt cccggagcat cagatccatc tagcgcgagc cacgctttgc ggaccgttgt
     8821 cacgcacgcc tccatgagcc tttgagtccc gcggtttgcc catttgtgtc gcgcgacacc
     8881 gagtttgcgt ctgcgccgcg gccgtttgtg ccccgcgaca cacaaacggt ctgaggacgg
     8941 tttgacgcgc tccgagggga gggtacgcct ctgcactagt tcaccgggta atcgactggc
     9001 cgcggtggat gacgttgcag gtagagcctt caagaaaagg agcattcatg gctgaattcg
     9061 aagagcgtag cgacaggttc acggcagtcg aggacaggtt cgcaggctac acggtctacg
     9121 accgggacaa cgacaagatc ggcaaggtag acgacctctt ccttgatgag aacgaccagc
     9181 cggagtactt cggcgtaaag atgggcttcc tcggtacccg ctcgaccttg atcccggcgg
     9241 agatcgctca gatcgacgag tctcagcagg ccgtgctggt ctcctccgag aagcagtacg
     9301 tcaaggatgg cccctccttc gacgacgacc gggagatcac gccggacttc gagcgggaag
     9361 tgcgcggcta ctatggagtc gatggtcagt cgccggacac cgagcgcggc tcatacggct
     9421 cctattactc ggacagcgac caggagttcg gcgcggagac cgaccgggac tccggcgagc
     9481 atcatcccgg tgttgcgatg ggcgaccgcg agggcggcga gttccgggag cacggcgtcc
     9541 acgaggaggg agcgaccgag ccgggtggcg acctcgacga ccaggacgag cttcgcgtgc
     9601 agcgcaccga ggaagagctt cgggccggca ccacggagcg cgaggccggg cgcatgaacg
     9661 tccgcaagcg cgtgcgcacc gaccgcgaga gcataggcgt gcccacgaag cgcgaagagg
     9721 tccacgtcga gcgcgtgccg gtagaccgcg aggctggcga gacggagatc ggcgacgacg
     9781 agatcagcgt cccgatcacc gaggaagagg tcgtggtcga gaagcggccg gtggtgaagg
     9841 aggagatccg tctgcgcaag gacgtggtcg aggacgagca agtcgtcgag gaagacgtcc
     9901 gccgcgagga agtggacatc gacgacgcca cggagcgccg ccgcgacgtc tagcatcaca
     9961 ccccctatga ggtttgcgga ggcgaagccg gcagatgtcc ggcttcgcct ccgctgtcag
    10021 ccgggaacga tggagcccgc cggttccatg acctgcgtgg tatccagcaa agaccggccg
    10081 tccagaggca ggacgctatc gagaagatcg cctgaaagcg caacattagc aggaggtaag
    10141 agatgaagga cagcgaacag cggggcaacc gggacgcgcc gagggtggag gagaacgaga
    10201 cgcgctccga agaccggcgc ggcgaatccg ccccccgacg ggttggcgac gtgcgccggg
    10261 accacgaggc ggagcgtcac cgcagggagt ccgagcttgg cacgagctgg acgagcgtaa
    10321 ccttcggctg gctcacggcg ctcggcgcgg gcctcatcct tagcgggatc gtcggcggta
    10381 tcgtgggagc gatactgggc gcgatgggcg tcaagggcgg cacggagggc ggcgtcgcgg
    10441 cgcttgtggg gctgctgatc acgctgcttc tggcgttcct gatcggcggc tacgtcgccg
    10501 gaaggatggc gagccgcgcc ggcgtcaagc acggtctgct ggtcccgcta ctggccctcg
    10561 tggtaacgct gctcctggcg gccatcggca cggctatcgg ctacagcttc atcgaccagc
    10621 tcagcggcgt cacgctgccg aagatccccg gcggcgtcca gaaccagatc ccgcagcaga
    10681 acctcgccac gctcctgacc gtccccggca tcctcgccct gctcttcccg ttcatcggcg
    10741 gggctattgg gggcgcgtgg ggagcaaaga ccggccgtaa ccgaccgtac taaaagtcca
    10801 gaagaggtgt ggcggacgat ccgtcacacc tcgatctata caaaggagga aaacgcatga
    10861 acatacttca gtcattgcag cagggggtga gcgccttctt cgcgttcatc ccgcagctaa
    10921 tcggcgccat cgtcatcctg atcgtcggct acatcgtagc gaaggtcctg gaagcggtcg
    10981 tcggtagggt cctgaaggct ataggcttcg acggctggat ggagaagagc ggtatcaagc
    11041 agtttttcga tagggcgcag actagacaga ccccggcgac catactgggc aagctggtct
    11101 tctggttcgt cttcatcatc gcgatcacga tggctacaga cgccctgggc ataagccagg
    11161 tctcggccgt gttcagccag ctaatcgcct acatccccaa catcatcgcg gcggtcttga
    11221 tcctgatcct ggccggtctg ctcgcccagt tcgtggccgg tctggtgcgg ggagctacgg
    11281 gcgtagatat cctcggcacg gtggcgcagg tcgcgatcat ggtctacgcg gtcttcgccg
    11341 ctcttacgca gctcggcata gcggtgcagc tcaccgcccc gacgttcctt atagtgctgg
    11401 gcgccgtggc cctcgccgcc gcgatagcgt tcggctgggg ttcgcgcaac gtggcccagg
    11461 acatagtgga gaaggcctac gcccgccggg gcgaggcgca ggagaagatc cagcgcgagc
    11521 gccagcggag cgactccgac tccgacgccg acatcggcac cggccgcccg tcggtacacc
    11581 gccgcgaggt ctagatacac ctccagacta cgttccgaaa acgagagtgc cgaccgggag
    11641 ggtgtccatt gcggacgccc tcccgcttca gatcagagcg gctgtacgca ccgctcgtcg
    11701 ttgttggccg ggcggttgac gcgggcgctc accgggtagg cgtccatctc atccggcgag
    11761 aaaggttcca acaacgagat cgcctcttgc gcctcggcct ccgggtcgag ccagggatcg
    11821 taggcgtccg gcggcagtat gacgggcatg cggtcgtgga tccccgcgac gagcgggttg
    11881 gcgcgggtgg tgattatggc gcaggagtgg acttcttcct ccggaccgct ccagctctcc
    11941 cggatgcccg cgaacgcgaa gggctcgccg ccgcgcacgg tgatgtagta cggctgcttt
    12001 ccgccattcg agcggcgcca ctcgtagaaa ccgtcggcgg ggatcaggca ccgccggcgg
    12061 cggaaggctg aacggaagga aggcttctcg ttcacggttt cggcgcgggc gttgatcagg
    12121 cgcgccccga tgctatcgtc tctggcccag gacgggatga gtccccaccg caacgtctcc
    12181 agcctaccgc tacccgttac ggcagccacc agttgcgtcg gggcgatgtt gtagctggac
    12241 gagagacgcg gcggatattc cgtcaggccg aaatgcttca ccagcgtatc cagcggggtc
    12301 ttcaacgtgt aacgtccgca catggctcgg aacgctccct tcgcatcgtt gcttgctaca
    12361 cggattctac ttgagaggtc ccggattcca ccggttctct cgcggcgcgg gacggacggg
    12421 ggtatgcttc cgggtatgat ctcctggttc cgccgcaggg gtaatggtat ccgcctggag
    12481 ctgggacagt ctctgggagc gttcaccagc ccggacggcg atatctcacc cctgttcgtc
    12541 ttcttcgagg tggcgaacgc cggcggcgag gacgtcacgc tcgccgcagt gcgcgtgacc
    12601 ccgagaggta tcccgcatac cgtcctggcg gacgcagcga agggcacgct ggaagggggc
    12661 cacgaccttc cccgcactct ggcgcccggc gagtccatcc ggttccaggt gagagccaag
    12721 accctggccg gcagggctcg cgacgcaggt cacagcggac gggcgcggct ggagttcgcc
    12781 gccgaggaca cgagcgggaa ccgtcaccgc aaggtcttca ggttccgggt ggacgagtac
    12841 ctgcggctaa aggacgagta agaaacgtaa gaaacggcgt gccccggaag tcgggatcag
    12901 tgccggtatc agagacgggt ggagcggagt tgtctgcggg ctccgggctc gcacgtcgag
    12961 ggtagcggcc cccgcagaag cctctcggcc ctatccacga tagagaggat atcgtcgagg
    13021 tccacaccct ccatcgtgga gataacgccg ccgaggcaga ccgtcgcctg ggtctccgcg
    13081 taggggcgga actcgatcca ggctctctcg caggagccgg ggagacagtc cgacctgcta
    13141 cagaacacca ggtccggttc gagacgcgat acctccgatt ccagaagatc gggctccagg
    13201 atctccactc tcaggtgcgg ccggagctcc tcgatagcca gcccgataac ctccctataa
    13261 gatctcggct cgatcgctat gaggaccttc atactcgcca actcccttca cgccgtatcg
    13321 ggagattcga cgagcgatcc tctcgcggtt cctgttcccc agcacgttcc atcccgatgt
    13381 ccataactac ttatgcagta acggacccgg tttcgtacat tttccggccg aatttcgaaa
    13441 aatctttccg cacaggagcc cggaacggcg ttcggacctt cagaccttgc ggcggcggtt
    13501 cccgaagagc gtctcgcggg tgtcgtcatc catgtcccgg tcgagcttgc ggcgcagttc
    13561 cttgccaacg tccagggcgc gccgcacggc gggacgggag cctacggcct cgaaccagcg
    13621 cttcaggttc gggaagtcgt ccatgtcctg cccctggttc tcgtagggga cgacccaggg
    13681 gtagaccgcc atgtccgcga tcgtgtactc gccgccggcg aagaattcgg cctccgagag
    13741 ccgctcgtcc atcacgccgt agagccgggc ggcctcgttc gtgtagcgct ctatggcgta
    13801 ggggatctca tccggcgcgt agttgcggaa gtggtgcgtc tgaccgagca tcggtcccac
    13861 accgcccatc tggaacatca gccactggag gaccagatac ctgtcgcgag gggattgcgg
    13921 gaagaaccgg ccggtcttct ccgccaggta gatcaggatg gcgcccgact cgaagaggga
    13981 gatcggctcg ccgtccggtc cttccgggtc gacgattgcc ggcatcttgt tgttcgggct
    14041 gatcttcagg aactccggct cgaactggtc gcccgccgcg atattgatag gcttgacgtt
    14101 gtacggcaac ccggcctctt cgaggaagat ggagaccttg tgtccgttcg gcgtcggcca
    14161 gtagtagagg tctatgggct gcgtcacgtc cgctcctccg tttctcactc cgactctcct
    14221 gcttgctgcc gcgccgagcg taccatgtcg tcgccggaac gtccctcgac atctcggcgc
    14281 tcgtccatct tcactatcgt gtagacccgt ccgatcccga gcccgaagac tgcctcctgc
    14341 gccctgccga gcgccgcgta gaactgctct gcgctctcgg cctcgaccgt cgtacccaga
    14401 gcggagaggc tgcacttcag cccggacacc tccagttcct taacggctgc ggctatgtag
    14461 cggctaacgc tcggcgtctc cgtcccaata gggatgatgc cgacttcggc tatgatcatg
    14521 aggtctcctt ccgtgagata tatacgctcg acgaagttta tacccggaga tcatgcctat
    14581 atctccggcc gagcgcccat tcgcgggact caagctatcg ggggcggacc ggtaccagac
    14641 tcttccggcg gcgccacaca aaccagagcg cgcccgagat catggccgcc atcgccgcgt
    14701 actggagtac cggggcgtac tccctgacga gcccccactg ggtccccagg acccatccga
    14761 gccctat
//
LOCUS       DASSMB010000062         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_521064, whole genome shotgun sequence.
ACCESSION   DASSMB010000062 DASSMB010000000
VERSION     DASSMB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521064"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFJ72_04345"
     CDS             complement(<1..498)
                     /locus_tag="VFJ72_04345"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET7478731.1"
                     /translation="MNPKKILGVGEHRPYPLPSGSWAISMSWHDLLFMHWPVPQDWLR
                     SLVPPALQLDTFDGSAWLGVVPFRMTGVRPRFLPGVPPLSNFPEINLRTYVTAGGKPG
                     VWFFSLDARNPVAVRLARATFGLPYFDAKMSCRTVGDEIHYRSVRTHRNASPAEFTAR
                     YRPSGG"
     gene            complement(553..1059)
                     /gene="thiW"
                     /locus_tag="VFJ72_04350"
     CDS             complement(553..1059)
                     /gene="thiW"
                     /locus_tag="VFJ72_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET7478732.1"
                     /translation="MANATRSLSGSATRKLVLAALFAGMGVLLSSFAIPVGGAKVFPF
                     QHAINAVAGVLLGPWWAAGSALVTATLRVSLGTGSLFAFPGSPFGAIAVGIAYRVLRR
                     DEAALFEPVGTVLVGATLGAVLISPLLDGAGGGILVLMGLFAASSIPGAIIGYLVLKA
                     LRKAGYTV"
     gene            complement(1052..1849)
                     /gene="thiM"
                     /locus_tag="VFJ72_04355"
     CDS             complement(1052..1849)
                     /gene="thiM"
                     /locus_tag="VFJ72_04355"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET7478733.1"
                     /translation="MNAVEILRRLAEEKPLVHHITNYVTVNLVANVTLCTGALPVMAH
                     SAEEVEEMEGLASALVLNIGTLDSRQIEAMLLAGKRANERGIPVVLDPVGAGATKLRT
                     RTAERLLSELQVAAVCGNAGEIATLAGLSAEVRGVESLAGDAEAAVTGAARALGITVA
                     ATGPTDYVSDGERILAVENGHPLMGRVVGSGCASTAAVGCFAAVGGGEVETVAAALAY
                     FGRAGEVAAEKADGPGTFEPRFLDALAALADDPSMVGETRIKEVGHG"
     gene            complement(1846..2640)
                     /gene="thiD"
                     /locus_tag="VFJ72_04360"
     CDS             complement(1846..2640)
                     /gene="thiD"
                     /locus_tag="VFJ72_04360"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7478734.1"
                     /translation="MKRVMSIATSDSGAGAGIQADLKAFLRCGVYGTTAIVATTAQNT
                     VGVNAIFPFPPQVAVDQIEAIVEDIGTDAVKTGMLFNAGIISAVAETIERLNLPNLVV
                     DPVMVAESGAVLLEPDAVETYKGELFPLATAITPNVNEAFALLGEERDEDHIEDCAVT
                     LRELGPKAVIITGGHTESGADLLYDGREFVRIEGPIHPSGNAHGSGCTHSSSLASFLA
                     RGFGLEEAARRARAVASEAVEYGLGEIGAGAGPVHALGKILKGVEE"
     gene            complement(2644..3630)
                     /locus_tag="VFJ72_04365"
     CDS             complement(2644..3630)
                     /locus_tag="VFJ72_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7478735.1"
                     /translation="MKKFLTLILICVLCAGLAACGGAASDSGKGPQKVTLTLDWYPNA
                     DHAGVYAAKEEGYFKDAGLDVQIRQPSDPAAVLQLVAAGRSEFGISYENEMTNAGIRD
                     IPVQSVMAIMQEPLNSIISLKKSGITSPEDLAGKKIGYAGQSFGTAVIDTVLRKAGKD
                     PSSAEKVNVGYDLRPALTSGRVDAIVDAYWNIEAVELKQKGFETNVIRLPQVGVPNYN
                     ELVVATSDSYAKENPETVRKFVGALVKGHRYAVEHPEAAKDALLKANKELDPRTAEET
                     LKLTTPVFKAARIGYQDPKEWQAYVDWAVKNDVLKKPVEVDKVMTNKFLPKG"
     regulatory      complement(3705..3821)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3838..>4230)
                     /locus_tag="VFJ72_04370"
     CDS             complement(3838..>4230)
                     /locus_tag="VFJ72_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7478736.1"
                     /translation="VLVVILFCFFPITVATVDGLRATDPDLIRLMRSFGASRRRTFFM
                     VEAPGALPFLFGGLRLAVTYCVIGAVIGEWVGSSKGLGFLMIQDKAQFEIPRLFAEIA
                     VLSVMGISLFLLVALVQRLTAPWTLRER"
BASE COUNT          689 a         1443 c         1392 g          706 t
ORIGIN      
        1 cccgccggac ggccggtagc gggccgtgaa ctctgcgggc gaggcgttgc gatgggtgcg
       61 gacgctccgg tagtggatct cgtctcccac agtccggcag gacatcttcg cgtcgaagta
      121 gggtaggccg aaggtggcgc gggcgaggcg gacggcaacg gggttgcggg cgtcgaggga
      181 gaagaaccag acgccgggct tccctccggc ggtgacgtag gtgcggaggt ttatctccgg
      241 gaagttcgag agaggcggca cgcccggcag gaagcgcggc ctcacgccgg tcatgcggaa
      301 cggtacgacc ccgagccacg cgctgccgtc gaaggtatcc agttgcaggg ccggagggac
      361 gagcgagcgg agccaatcct gcgggacggg ccagtgcatg aagagcaggt cgtgccagct
      421 catcgagatc gcccacgacc ccgagggaag cgggtacggg cgatgctcac cgacgccgag
      481 tatcttcttg gggttcatcg cgccggtcat accgaaaaga tagcccggtt ctaatctaac
      541 gtccaggaac gctcacaccg tgtagccggc tttcctgagc gccttgagga cgaggtagcc
      601 gatgatggcg ccggggatgc tggaagcggc gaagagtccc atgaggacca ggatgccgcc
      661 gcccgcgccg tcgaggagcg gtgagatcag gaccgcccca agcgtcgccc cgacgagcac
      721 cgtcccgacc ggctcgaaga gcgcggcctc gtcgcggcgc agcacgcggt acgcgatccc
      781 cacggctata gccccgaacg ggcttcccgg aaacgcgaac aggctccccg ttcccagaga
      841 gacccgcagc gtcgccgtga cgagcgccga acccgccgcc caccacgggc cgagcaggac
      901 cccggccacc gcgttgatcg cgtgctggaa cgggaacacc ttggccccgc cgaccgggat
      961 cgcgaacgac gagagaagca cccccatccc cgcgaacagc gccgcgagca ccagcttccg
     1021 ggtcgcgctc cccgacagac tcctcgtcgc gttagccatg tccgacctcc tttatccggg
     1081 tctctcccac cattgaaggg tcgtccgcga gagccgccag agcatcgaga aaccggggct
     1141 cgaaggtccc aggcccgtcc gccttttcgg cggcgacctc ccccgcgcgc ccgaagtacg
     1201 ccagcgccgc cgcaacggtc tcaacttctc cgcccccgac ggcggcgaag cagccaacgg
     1261 cggcggtcga ggcgcagcca ctcccgacta cccggcccat caggggatga ccgttctcca
     1321 cagcgagaat gcgttcgccg tcgctcacgt agtccgttgg accggtcgcg gcgacggtga
     1381 tgcccagcgc gcgagcggct ccggtcaccg ccgcttccgc gtccccggcg agactctcga
     1441 cgccgcggac ctcggcggag agcccggcga gcgtggctat ctcgccggcg ttgccgcaca
     1501 cggcggcgac ctgcagctcg gagagcaggc gctcggcggt ccgggtgcgg agcttcgtgg
     1561 cgcccgcgcc gaccgggtcg aggactacgg ggatgccgcg ctcgttcgcc ctcttcccgg
     1621 cgaggagcat cgcctcgatc tggcggctat ccagcgtccc gatgttcagg acgagcgcgg
     1681 aggcgaggcc ctccatctcc tccacctcct cggcggaatg ggccatcacc ggtagcgcgc
     1741 cggtgcagag cgtcacgttg gcgaccaggt tgaccgttac gtagttggtg atgtggtgga
     1801 cgagcggctt ctcctccgcc agcctccgca ggatctctac cgcgttcatt cctccactcc
     1861 cttcagtatc ttccctagcg cgtggaccgg cccggccccg gccccgatct cacccagccc
     1921 gtactcaacc gcctcggagg cgacggcgcg agcccgccgg gccgcctcct ccagcccgaa
     1981 gccgcgcgcc aggaagctcg ccagcgacga cgagtgcgtg cagcccgacc cgtgcgcgtt
     2041 cccggaaggg tggatcggac cttcgatcct gacgaactcc cggccatcgt agagcaggtc
     2101 cgcgccggac tccgtatgcc cgccagtgat gatgacggct ttcggtccca gctcgcgcag
     2161 cgtaacggcg cagtcctcga tgtggtcctc gtcccgctcc tcccccagca gagcgaaagc
     2221 ctcgttcacg ttcggggtta tcgcggttgc gagcgggaag agttcccctt tgtaggtctc
     2281 gacggcgtcc ggctccagca agactgcccc gctctcggcg accatcaccg ggtcaacgac
     2341 caggttcgga aggttcaggc gctctatggt ttcggcgacg gcggagatta tcccggcgtt
     2401 gaacagcatc ccggtcttta ccgcgtccgt gccgatgtcc tcgacgattg cctctatctg
     2461 gtccacggcg acctgtggcg ggaacgggaa gatcgcgttg acccccaccg tgttctgggc
     2521 cgtggtggcg acgatggcgg tcgtcccgta gacgccgcag cgcaggaacg ccttgaggtc
     2581 cgcctgaatg ccggccccag cccccgagtc gctggtcgct atgctcatca cccgcttcac
     2641 ggatcaaccc ttcggcagga acttgttggt catcaccttg tccacctcca ccggcttctt
     2701 gagcacgtcg ttcttcacgg cccagtccac gtaggcttgc cactccttcg ggtcctggta
     2761 gccgatgcgg gccgccttga agacgggcgt cgtcagcttc agggtctcct ccgcggtcct
     2821 ggggtcgagc tccttgtttg ccttcagcaa ggcgtccttc gcggcctcgg ggtgctcgac
     2881 ggcgtagcgg tggcccttga cgagcgcgcc gacgaacttg cggaccgtct cggggttctc
     2941 tttagcgtag gaatcggacg tggcgacgac cagctcgttg tagttgggga cgccgacctg
     3001 ggggagcctg atgacgttgg tctcgaagcc cttctgcttc agctcgacgg cctcgatgtt
     3061 ccagtaggcg tccacgatgg cgtccacccg gccggaggtc agggccgggc gcaagtcgta
     3121 gccgacgttc accttctcgg cgctgctggg gtctttgccg gccttgcgaa ggacagtgtc
     3181 tatgacggcg gtcccgaagg actgtccggc gtagccgatc ttctttccgg ccaggtcttc
     3241 cgggctggtg atgccggact tcttcaggct gatgatggag ttcaggggct cctgcattat
     3301 cgccatgacg gactgtacgg ggatgtcccg gataccggcg ttggtcatct cgttctcgta
     3361 ggagatgccg aactcgctgc gtccggcggc cacaagctgg aggacggccg ccgggtcgga
     3421 gggctggcgg atctggacgt cgagccccgc gtccttgaag tagccttcct ctttggccgc
     3481 gtacacgccg gcatggtcag cattcgggta ccagtcgagc gtgagcgtta ccttctgggg
     3541 acctttcccg gaatccgagg cggccccgcc gcaggccgcg agcccggcgc aaaggacaca
     3601 gatcaatata agcgtaagga actttttcat cacaacctcc gataggtcta acagagcgaa
     3661 tccttgtggc ggacgagcgc cgccggaggc tgtgttacgg gacgggttcc atcaggttca
     3721 ctcccttccg gtggggttac ccacatcagg ttctaagcgt ctcggctctc gccgactctc
     3781 agccgccccg ctcttccggg gaagctccgc cgtgtatgtc gtgccggcca ctatagccta
     3841 gcgctctctc agggtccagg gagcggtgag gcgctggacg agggcgacga ggaggaagag
     3901 cgagatcccc atcacggaga ggacggcgat ctcggcgaac aatcgcggta tctcgaactg
     3961 ggccttgtcc tggatcatga ggaacccgag ccccttgctg gagcccaccc actccccgat
     4021 gaccgcccct atgacgcagt acgtcacggc cagccgcagc ccgccgaaga ggaacggcag
     4081 cgcccccggc gcctcgacca tgaagaacgt cctgcgccga ctcgccccga aggagcgcat
     4141 caggcgtatg aggtccgggt ccgtggcccg cagaccgtcc acggtggcga ccgtgatcgg
     4201 gaagaagcag aacagtatga ccacgagcac
//
LOCUS       DASSMB010000063        11024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1281706, whole genome shotgun sequence.
ACCESSION   DASSMB010000063 DASSMB010000000
VERSION     DASSMB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 11024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11024
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1281706"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VFJ72_04375"
     CDS             complement(<1..1264)
                     /locus_tag="VFJ72_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7478737.1"
                     /translation="MAVRQDEKTMRKETKATGRTDVLIVGAGPTGLTLAYQLKRLGVD
                     FRIVDKNPEPSTTSKAIGLQYRVSEVLAWMGLAERFLARSATSQKVNMYVGGERILQL
                     KLSVLDMRGGQDAFVPRMMIIPQSETEALLIGALEEHGGHIERGTVFLGFEQNRDRVV
                     SRLRYPDGSEERIESRYLVSCEGAHSIVRKQSGISFAGETYPFSFVMADVELDWDRSH
                     DEIYSWLHEKGTLSAGPLPGNRWRLFIEMEEGQRVPCVTLELAQRLLAERTGDYKTHA
                     TNPTWLSEFRINSRMVDRFREGRVFLAGDAAHVHSPSGGQGITTGMQDAYNLSWKLAQ
                     VLNEGAPDSLLDTYSEERLPIVRSVLETTDRNTGILFPRGRFGRLIRNAVVLPLLRRD
                     RVQDRLVRKMSQLDMNYRGASLSVDQNAD"
     gene            complement(1302..1799)
                     /locus_tag="VFJ72_04380"
     CDS             complement(1302..1799)
                     /locus_tag="VFJ72_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7478738.1"
                     /translation="MLKKEAKELQVLETREALDVTSPDRGRRASTVVLHHAAVFVENL
                     DRSASFYADVLGLEVTDRAHLPAIGIEQVFLSAGHKHADLVLARRLNGSVAPPDKREL
                     FHLAFELPREQPFEEFLERVSREGVSVVAGPMERPARFDGSGSRTAAYLRDPDGYLLE
                     VTQEH"
     gene            1986..4370
                     /locus_tag="VFJ72_04385"
     CDS             1986..4370
                     /locus_tag="VFJ72_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7478739.1"
                     /translation="MDEARITDFGASLRRLREASGLTQEELASRAGLSSKGIGALERG
                     ERKRPHPHTVNALVDALDLAGPERDAFFGAVPRRIGMAFVPVADLEEAIPALPAPLTL
                     LIGRERDVAVVDSLLEGDGARLLTLTGTGGVGKTRLALEVAGQARERFPDGVAFVPLA
                     PVADPALLVPTVVQILGLREAGGETMRELVQRHLRGKRLLLVLDNFEHLLEAAPEISV
                     LLSTVPSLKILATSRAPLRVRGEHEYPVAPLAVPYPTRVTDTDVVAAAPSAQLFAERA
                     REAAPSFSLTRKNAAAVAAICWRLDGLPLALELAAARVRFLGPTELLSRLDQVLQAEG
                     ARDLPERQRTMRATLDWSYGLLSEEEKPLFGHLSVFAGGFTLEAAEAVGAQVDETGTR
                     EVLGLLEGLVAQSLVMAEASPEGGEADETRYGMLEPVRQYARAKLEEVGEGEEERRRH
                     AMFFLDLVERAAPELRGPWQVEWLHRLDAEHGNLRAAMAWALSAGEIETGARLAWALF
                     VFWWMRGYYAEGRRASERVLAADAELTASLRGKMLFVAGMMCNAQGDHERGQTLDEEG
                     VRLLREAGDRPTLAIVLAALGYASVRRRDYDRATVLLEESLELYRELEDRWGTAKLLN
                     NLGRLRTLRGDSGDAKPLLEEGLALSKELGDTSAIAEALHNLAVAALLPNEHDRAAVL
                     FEESLTLAAEVGHRSIVADCLEGLACAEAGRREPQRSARLWASAEALRETAGASSEGT
                     ERPLYEAYLISARAALDETMFEAAWASGRAMTLQQAVAYALEERELAPEPPTAD"
     gene            4466..6109
                     /locus_tag="VFJ72_04390"
     CDS             4466..6109
                     /locus_tag="VFJ72_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET7478740.1"
                     /translation="MDTRKSAKDRWEEAYSKTHERDVEFSTMSGIPIKPLYTPDDVEG
                     DYDEKLGYPGEFPYTRGVYPNMYRGRLWTVRQFAGYGSAEDTNRRFKYLIEHGQNGLS
                     VAFDMPTLMGLDSDSPLSLGEVGTEGVAIDSLEDMERLFDGIQMGEITTSMTINAPAM
                     VLLAMYVVAAEKQGVPPEDLAGTNQNDILKEYIAQKEWLFPPAPSMRVFKDMLVYATE
                     HMPRWNTVSISGYHIREAGSTAAQELAFTLADGFAYVEAGIEAGLDVDDFAPRFSFFF
                     NSHIDFFEEICKFRAARRIWATVLKEKYGAKDERSLRMRFHTQTAGVSLTAQEPYNNV
                     ARTAFEALAAILGGTQSLHTNSLDEALALPTEEAVRIAVRTQQIAALETGVTNTMDPL
                     GGSYFVEALTDELERQAYEYFRQIDEMGGMIPAIEQGFPMREISEASARYQRELEEKK
                     RYMVNVNVYEPQDEQEMELHRVDPEVSQGQVDRLKELKERRDNDAVERCLEELKETAR
                     GDGNTMYPILDAVRAYATVGEISSALQEVFGEYRETPVI"
     gene            complement(6151..6927)
                     /locus_tag="VFJ72_04395"
     CDS             complement(6151..6927)
                     /locus_tag="VFJ72_04395"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET7478741.1"
                     /translation="MIRNLSGRGWLASLAVLALAMLMAGAAPAQATVPDTIQNVRFGD
                     HTTFERAVIDLGHGGVPGQVAPTFSSSYRQGDWIVRVDLLSVTATARTDGSGLGRAIS
                     KYYVVRSRSSTGNMFVDFHLTGAAKSVNVFKLDNPARIVVDVTPGGTTLFPRPVTGAS
                     TVVTRPRTDMLVGPDTFIVSGYGRPFEASGVWRIKNASGTVVRRGTYTTSDWAATWGA
                     YKFSAGYPSSLSGHSGTLQVGELSPKDGSFMGVSVPLRFR"
     gene            7327..7641
                     /locus_tag="VFJ72_04400"
     CDS             7327..7641
                     /locus_tag="VFJ72_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478742.1"
                     /translation="MMHARVVDLRVRPMDTKEVVRIYRDSVLPAAREQEGFMGALLLT
                     DSTGIGISITLWETEEDREAGEASGFYKEQINKFADHLVETPVRKHYDVGVLHYEVSA
                     LA"
     gene            7779..8783
                     /locus_tag="VFJ72_04405"
     CDS             7779..8783
                     /locus_tag="VFJ72_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7478743.1"
                     /translation="MTIRETRTRHETLGLDEDDLLRIYRHMLVARRVDERSWILNRQG
                     KAAFVISCQGQEAAQIGAAYNLRPGYDFLYPYYRDSGMTLLLGQTPRDQFLSILGKKE
                     DPNSGGRQMPGHYSSRELNIVTASAPVGVQYPQATGSALAFKMRGEDGVVMACGGEAS
                     TSGGDWHEAMNFAGIHDLPVIFLIENNVYAISTPEPLEVAGSIARRAEGYGFPGHAVD
                     GNDVLAVYEVASEAVERARRGDGPTLIEAKTYRITAHSSDDDDRRYREREEIEEWRLK
                     DPILRFEKYLMENGLLDEDRKEEIDAGIRAEVAEASEYAENAPFADPEDVLRGVYAEG
                     "
     gene            8793..9779
                     /locus_tag="VFJ72_04410"
     CDS             8793..9779
                     /locus_tag="VFJ72_04410"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7478744.1"
                     /translation="MAEMNLLQAIHEGLAEEMRADETVMVLGEDVGRAGGVFRVTQGL
                     QDEFGAERVMDTPLAESLIVGSAIGLSVNGMRPVAEIQFGDFITPAFDQIVNEAARFY
                     YRSNGAYKCPITIRAPYGAVHGGALYHSQSIEAWFCNTPGLKVVAPTFPADAKAMLKS
                     AIRDPNPVLFLEHKRTYRLIKEDVPDNGREVPIGEARVHRQGADITVVSYGLMLYTAL
                     EVAQKLSEEHGIEAEVVEPRTLYPLDKETILDSAKKTGKFLVISEANLTGSVSGEIAA
                     IVASEAFEWLDAPVSRLGAPDVPAAAFARPMMDYFVPDAEKIEAAMLELARY"
     gene            9801..>11024
                     /locus_tag="VFJ72_04415"
     CDS             9801..>11024
                     /locus_tag="VFJ72_04415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET7478745.1"
                     /translation="MAKPVTMPRLGESVTEGTIIRWLKAEGDEVAMDEPLAEVDTDKV
                     NAELPSPVAGRVEKLLVSEGATVDVGVEIALVADAESAPASSDAPPRENVAAGAPTEE
                     FPAAGTVSQPVASGERSSVAEARSVGSGGNGRGGIPAAETLRLTRSSPVVRRLAAEHG
                     VDIQEIPGSGTGGRVTKKDIERYIEERGTRREEYAPPGLREEREAFAPPEAPPEPEPS
                     RERVTVYEGDRVQDLTSIRRAIANRMSASKREAPHAWTMVEVDVTGLAALREREKAAF
                     AEREGVKLTYLPFIVQAVVESLKEHPVLNSVWDGDRIVLRKRINIGVAVNLEDALIVP
                     IIPDADELNIVGLVRKIDDVVRRARSRQLGPEDISGGTFTVNNPGSLGSVVSTPIINY
                     PQAAILSAEAIVKRPV"
BASE COUNT         2035 a         3501 c         3646 g         1842 t
ORIGIN      
        1 catccgcgtt ctggtcgacg gagaggctcg cgcctcggta gttcatatcc agttgagaca
       61 tcttgcgcac gagccggtcc tgcaccctat cgcggcgtag cagcggcagg acgacggcgt
      121 ttcggatgag cctgccgaag cggccgcgag ggaacaggat tcccgtgttc cggtcggtcg
      181 tctccaggac gctcctgacg atggggagtc gttcttcact gtaggtgtcg agcagggagt
      241 cgggcgctcc ttcgttcagt acctgggcca gcttccagga gagattgtag gcgtcctgca
      301 tcccggtcgt tatgccctgt ccgccgctag ggctgtggac gtgggccgcg tcgccggcga
      361 ggaagactcg tccctcgcgg aaccggtcca ccatgcggct gttgatcctg aactccgaga
      421 gccacgttgg gttggtcgcg tgcgtcttgt agtctcctgt ccgctcggcg agcaggcgtt
      481 gcgccaactc tagcgtgacg cacgggacgc gctgtccttc ttccatctcg ataaacaggc
      541 gccacctgtt gcccggcagc ggacccgcag agagggtgcc cttctcgtgg agccacgagt
      601 agatctcgtc gtggctccgg tcccagtcaa gctcgacgtc ggccatgacg aaggagaacg
      661 ggtaggtctc gcctgcgaac gagatacccg actgcttgcg gacgatactg tgcgccccct
      721 cgcagctgac gaggtagcgc gactcaatcc tctcctccga gccgtccggg tacctgaggc
      781 gcgagacgac ccggtcgcgg ttctgctcga agccgaggaa gaccgtgcca cgctcgatgt
      841 ggcccccgtg ctcctcaagg gcgcctatga gcagcgcctc ggtctcgctc tgcgggatga
      901 tcatcatgcg cgggacgaag gcatcctggc caccccgcat gtcgagcacg ctcaacttca
      961 gttgcaggat gcgctcgcct cccacgtaca tgttgacctt ctggctcgtg gcgcttctcg
     1021 cgagaaaacg ctccgcgaga cccatccagg cgagtacctc cgagaccctg tactgcaagc
     1081 ctatcgcctt cgaggtcgtc gaaggttccg ggttcttgtc cacgatccgg aagtccaccc
     1141 ccagccgctt caactggtag gcgagggtga gtccggtagg tccagcgccc acgatcagaa
     1201 cgtccgttcg cccggtcgct ttcgtctctt tgcgcatggt cttctcgtcc tgtctaacgg
     1261 ccatctcttt ctcctttcat gggatttgcg tagatctccg tctagtgttc ctgcgtcacc
     1321 tccaagaggt agccgtcggg atcccggagg tacgccgccg ttcgcgatcc cgagccatca
     1381 aacctcgcgg ggcgctccat cggacctgcc accacggaca ccccctcgcg cgagacgcgc
     1441 tcaaggaact cctcgaacgg ctgttcgcgc ggcagctcga acgcgaggtg gaaaagctcg
     1501 cgtttgtctg gaggggccac ggacccgttc aggcgacggg cgagcaccag gtcggcgtgt
     1561 ttgtgtccgg cggagaggaa gacttgttct atgcctatcg ccgggaggtg ggcacggtcc
     1621 gtgacctcta ggcccagcac gtcggcgtag aacgatgcag agcggtcgag gttttcgacg
     1681 aaaacggccg cgtggtggag aaccaccgta ctcgctctcc gcccccggtc gggggaggtg
     1741 acgtccaggg cctccctggt ctccagcacc tgcaactcct tcgcttcttt cttcagcatg
     1801 gtgtctctcc tttttaatgc cttgttggtt gtcagtctgc gttccgagcc gaccgagaac
     1861 cacccacagg acagccacat tcactcccac aatcaatcgt ggaggtgtac gtgcggcgtc
     1921 tgctatcatg acctcgcgcc ttcggcacgg accgtgcgag gggctatgag ttcggggagc
     1981 gaggcatgga tgaggcaagg atcacggact tcggcgcgag cctgaggcgc ttgcgagagg
     2041 cttccgggct cacgcaggag gaactggcct cgagggccgg actttcgtcg aagggtatcg
     2101 gcgccctgga gcgcggtgag aggaagcggc cgcatcccca caccgtcaac gcccttgtcg
     2161 acgccctgga cctcgccggt cccgaacgcg acgccttctt cggtgcggtt cccagacgta
     2221 tcggcatggc cttcgtcccg gtggccgatc tcgaagaggc aatcccggcg ctgcctgcac
     2281 cgctgacgct gctcatcgga cgggagcgcg acgtcgcggt ggtcgactcg ctgctcgaag
     2341 gagacggtgc caggctcctc acgctcacgg gaacgggcgg tgtgggcaag actcgcctcg
     2401 ctctggaagt ggcgggccaa gctagggagc ggtttcccga cggcgtcgcc ttcgtgcctc
     2461 tggccccagt cgccgaccct gcgctcctcg ttcccaccgt cgtccagatc ttgggcttgc
     2521 gggaagcggg aggcgagacc atgagagagc tggtacagag gcaccttcgg ggcaaacgtc
     2581 tgctgctcgt gctcgacaac tttgagcacc tgctcgaggc cgctcccgaa atatcagtgc
     2641 tgctatcgac cgttccttcc ctcaagatcc tggctacgag ccgcgcccct ttgcgcgtgc
     2701 gcggcgagca tgagtacccg gttgcgcccc ttgctgtgcc gtaccccacc cgtgtaacgg
     2761 atacggacgt cgtggccgcc gccccatctg cccagctctt cgccgagcgg gcccgagaag
     2821 ccgctccctc cttctcgctc acccggaaaa acgccgccgc ggtggcagcg atctgctggc
     2881 gcctggacgg actgccgctt gccctggagc tcgccgcggc gcgggtgagg ttccttggtc
     2941 ccacggagct actctcgcgc ttggaccagg tcctgcaagc ggagggagcg agggacctcc
     3001 ccgagaggca aaggacgatg cgggcgaccc tcgattggag ctacggcctg ctctcggaag
     3061 aggaaaagcc gttgttcggg catctgtcag tgttcgccgg tggcttcacg ctggaggcgg
     3121 cggaggctgt gggcgcgcag gttgatgaga cggggaccag agaggtactg ggcctgctgg
     3181 aaggtctggt agcgcagtcg ctggttatgg ccgaggcttc accggaggga ggcgaagccg
     3241 acgagacgcg ctacgggatg ctggagcctg taaggcagta cgcccgagcg aagcttgaag
     3301 aggtaggtga aggtgaagag gagcggcggc ggcatgcgat gttcttcctc gacctggtgg
     3361 agcgggcggc gccggaacta cggggaccct ggcaggtgga gtggctgcac cggctcgacg
     3421 cagaacacgg caacctgcgc gccgcgatgg cgtgggctct ctctgcaggt gagatcgaga
     3481 caggagcccg cctggcttgg gcgcttttcg tgttctggtg gatgcgtggc tactacgctg
     3541 aaggccggcg cgcgtcggag agggtactgg cggccgatgc agagttgaca gcctctctga
     3601 gaggcaagat gctgttcgtg gcggggatga tgtgcaacgc tcagggagat cacgagcgcg
     3661 gccagacgct agacgaggaa ggcgtgcgcc tgctgcgcga ggccggcgac aggcctaccc
     3721 tcgccatcgt gctcgccgcg cttggctacg cttcagtgag gaggcgcgac tacgaccggg
     3781 caaccgttct actggaggag agcctagaac tgtacaggga actggaagac agatggggga
     3841 cggccaagtt actcaacaac ctcggacgcc tgaggacatt gcggggcgac agcggagacg
     3901 caaagcccct gcttgaggag ggtttggcgc tttctaagga gttgggagac acgagcgcca
     3961 ttgccgaagc cctgcacaac ctggccgtcg cggcgctcct gcccaatgag cacgaccgcg
     4021 ccgccgtcct gttcgaggag agccttacgc tggccgccga ggtgggccac aggtctatcg
     4081 tcgccgactg cctggagggg ttggcttgtg ctgaggcggg acgacgggaa ccgcaacgct
     4141 ccgcgcggtt gtgggcgtca gccgaagcac taagggagac tgcgggcgcc tcctcggagg
     4201 gtaccgaacg ccccttatac gaggcatacc tgatctccgc gcgcgccgct ctggatgaga
     4261 cgatgttcga ggcggcctgg gctagtgggc gggcgatgac cctccagcag gcggtagcat
     4321 acgccctgga agagcgagag ctcgcgcccg agccaccaac ggcggattga tgtaagggtc
     4381 gagaacttac atcctgccct ctggtggacg aatcgcttgt gctagagtct aggcgtcagg
     4441 ggacagagaa gcaggaggag cacgcatgga caccaggaag agcgccaaag accggtggga
     4501 ggaagcatac tcaaagaccc acgagcgcga cgtcgagttc tccacgatgt ccggcatacc
     4561 catcaagccg ctctataccc ccgatgacgt ggagggcgac tacgacgaga agctcggcta
     4621 ccccggcgag ttcccttaca cccgtggcgt ctacccgaac atgtaccgcg gccgcctgtg
     4681 gacggtccgt cagttcgcgg gttacgggtc tgccgaagac accaacaggc gcttcaagta
     4741 cctcatagag cacggccaga acggactctc cgttgccttc gacatgccga ccctgatggg
     4801 actggattcc gactctccgc tctctctcgg cgaggtgggg acggagggcg tcgccataga
     4861 ttctctggag gacatggaga ggctcttcga cgggatacag atgggcgaga tcacgacctc
     4921 catgaccatc aacgcccccg cgatggtcct gctcgccatg tacgtcgtcg ccgccgagaa
     4981 gcagggcgtc ccgcccgaag acctggccgg caccaaccag aacgacatcc taaaagagta
     5041 cattgcccag aaggagtggc tcttcccgcc cgcgccctcc atgcgcgtct tcaaggacat
     5101 gctcgtctac gccaccgaac acatgccccg ctggaacacg gtcagcatca gcggctacca
     5161 tatccgggaa gcgggctcga cggcggcgca ggagctggcc ttcaccctcg ccgacggctt
     5221 cgcctacgtg gaggccggga tagaggcggg cctggacgtg gacgacttcg cgccgcgctt
     5281 cagcttcttc ttcaactcgc acatagactt cttcgaggag atctgcaagt tccgtgccgc
     5341 gaggcgcatc tgggccacag tcctgaaaga gaagtacggc gccaaggacg agcgttccct
     5401 caggatgcgc ttccacaccc agaccgccgg cgtctccctc accgcccagg agccgtacaa
     5461 caacgtcgcc cgcaccgcct tcgaggcgct cgccgccatc ctcggcggga cgcagagcct
     5521 gcacaccaac tcgctcgacg aggcgctcgc gctgccgacg gaggaggccg tccgcatagc
     5581 cgtccgcacg cagcagatag ctgctttaga gaccggggtg acgaacacaa tggatccact
     5641 cgggggttcg tacttcgtcg aggcgctgac cgacgagctg gagcggcagg cgtacgagta
     5701 cttccgccag atagacgaga tgggcggcat gatcccggcg atagagcagg gcttcccgat
     5761 gcgcgagatc tccgaggcct ccgcccgcta ccagcgcgag ctggaggaga agaagcgcta
     5821 catggtgaac gtcaacgtct acgagccgca ggacgagcag gagatggagc tgcaccgcgt
     5881 ggacccggaa gtctcacaag gacaggtgga tcgtctgaaa gagctaaaag agcgccgcga
     5941 caacgacgcg gtcgagcggt gcctcgaaga gttgaaagag acggcgcgcg gcgacggcaa
     6001 caccatgtac ccgatcctgg acgccgtccg ggcatacgcc acggtggggg agatctcctc
     6061 ggctctacaa gaggtcttcg gcgagtaccg cgagacgccg gttatataag aacgagcgag
     6121 ccggagtttc gtgctccggc tcgaatcgac ctagcggaac cgcagaggga ccgagactcc
     6181 catgaagctc ccgtctttcg gcgagagctc gccgacctgc aaggtgccgc tgtgcccgct
     6241 caggctcgac ggatagccag cggagaactt gtaagctccc caggtcgccg cccagtcgct
     6301 cgtcgtgtag gtcccccggc gcacgaccgt accgctggcg ttctttatgc gccagactcc
     6361 gctggcctcg aacggcctgc cgtagccgga cacgataaag gtgtcgggac cgacgagcat
     6421 gtccgttctc ggacgggtca ccacggtgct cgccccggtg acgggcctgg ggaacagcgt
     6481 cgttcctccg ggggttacgt ccaccactat gcgggccggg ttatccagct tgaacacgtt
     6541 cacggacttc gccgccccgg tgaggtggaa gtccacgaac atgtttccgg tggagcttct
     6601 cgaacgcacc acgtagtact tcgagatcgc tcgcccgaga ccggagccgt ccgttctggc
     6661 cgtcgccgtt accgagagaa gatccacccg cacgatccag tcgccctgcc tgtaggacga
     6721 cgagaaagta ggagcgactt gccccggcac tcctccgtgg cccaggtcta tcaccgcccg
     6781 ctcgaacgta gtgtggtccc cgaaacggac gttctgtatg gtgtccggca ccgtggcttg
     6841 cgccggagcc gctcccgcca tcaacatcgc cagggccagg accgccaggc tcgccagcca
     6901 cccgcggccc gataaattcc tgatcattac cgcctccata gaaaagggcc gttgcccttc
     6961 ctcgatctgt tggaaagaag ctcaaaatag gtgtctgccg ctcgctgcta cggcgcgctc
     7021 acagcggcaa ggaaagccag cagcctgtaa cgcaaccagt acacgacaac cgtccactct
     7081 ccagcaaaac actcgttcgg atctcagcct caaccacaga ttagcccgcc ctgattacaa
     7141 accggaaaga gttggattaa ggcaatcgta atatatgtta ttttaagcag cctccagagc
     7201 acccgtctcg gatgatttcc cacgatgttt tccgtgtttg caggtgagtc tccgatctcg
     7261 ccagtccggg atataataga ggttgatacg tgagtatcag gcggttgggg tgacgggaag
     7321 gagtcggtga tgcatgctag ggtggtggat cttcgagtac ggccgatgga taccaaggag
     7381 gtagtccgca tctatcggga ctccgtgttg ccggcggcca gagagcagga ggggttcatg
     7441 ggtgctctgt tgttgacgga cagcacgggg atcgggatct ccataacgct ctgggagacg
     7501 gaggaggacc gggaggctgg agaggccagc ggcttctaca aggagcagat aaacaagttc
     7561 gcggatcatc tcgtcgagac gcccgtgcgc aagcactacg acgttggcgt cctgcactac
     7621 gaggtcagcg ctctggctta ggttgcatgg gcgggatgcc tgtcgggttc gggcgccgtg
     7681 gccgggtctg gaggaacggt cgtctgctaa gatctagctg tggaggaact gcctttcggg
     7741 gtcctcccag ggggaccttt gagtagagga ggaggtctat gactatccgg gagactcgca
     7801 cccggcacga gacgctgggg ctggacgagg acgacctgct gaggatctac cgtcacatgc
     7861 tcgtggcgcg gcgggtggac gagcggtcct ggatcctgaa ccgccagggt aaggccgcgt
     7921 tcgtcatttc ctgccagggc caggaggccg cccaaatagg ggccgcctac aacctccggc
     7981 ccggctacga cttcctctac ccctactacc gcgactccgg catgacgctc ctgctcggcc
     8041 agacccctcg cgaccagttc ctgagcatcc tcggcaagaa ggaagacccg aacagcggcg
     8101 gtcgccagat gcccggccac tacagcagcc gcgagctgaa catagtgacc gcttccgcgc
     8161 cggtcggcgt gcagtacccg caggcaaccg gcagcgccct ggcgttcaag atgcgcggcg
     8221 aggacggcgt ggtgatggcg tgcggcggcg aggcgtccac cagcggcggc gactggcacg
     8281 aggcgatgaa cttcgccggc atccacgacc tgcccgtaat cttcctcatc gagaacaacg
     8341 tctacgccat ctccaccccc gagcccctgg aggtcgctgg ctccatcgcg aggcgggccg
     8401 aagggtacgg cttccccggt cacgcggtag acggcaacga cgtgctcgcc gtctacgagg
     8461 tcgcaagtga ggccgtcgag cgcgcccgcc gcggtgacgg cccgacgctc atcgaggcca
     8521 agacctaccg cattacggct cattcttcgg acgacgacga ccggcgctac cgggagcgcg
     8581 aggagatcga ggagtggcgc ctgaaggatc cgatcctgcg tttcgagaag tacttgatgg
     8641 agaacgggct gctcgacgag gacaggaaag aggagatcga cgccgggata agggccgagg
     8701 tcgccgaggc gagcgagtac gccgagaacg cgcccttcgc cgatcccgaa gacgtgctcc
     8761 gcggcgtcta cgcggagggt tagggggtac ccatggccga gatgaacctg ttacaggcaa
     8821 tccacgaggg actcgccgag gagatgcgcg ccgacgagac cgtgatggtg ctgggcgagg
     8881 acgtgggccg ggccggcggg gtgttccggg tgacacaggg gttgcaggac gagttcggtg
     8941 cggagagggt catggacacg ccgctggccg aaagcctgat cgtcggctcc gccatcgggc
     9001 tctcggtgaa cgggatgcgt ccggtggcgg agatacagtt cggtgacttc ataactcccg
     9061 ccttcgacca gatcgtcaac gaggccgccc gcttctacta ccgctccaac ggcgcctaca
     9121 aatgccccat caccatccgc gccccctacg gagcggtaca cggcggcgcg ctctaccact
     9181 cccagagcat cgaggcctgg ttctgcaata cgccgggcct caaggtcgtc gcgcccacct
     9241 tcccggccga cgcgaaggcc atgctaaaga gcgccatccg cgacccgaac ccggtcctct
     9301 tcctcgaaca caaacgcacc taccgcctca taaaagagga cgtccccgac aacggccgcg
     9361 aggtccccat cggcgaggct cgcgtccacc gccagggcgc cgacataacc gtcgtatctt
     9421 acggactcat gctctacacg gcgctcgaag tggcccagaa gctctccgaa gagcacggca
     9481 tagaggccga agtcgtcgag ccgcgcacgc tctacccgct ggataaggag acgattctcg
     9541 attcggcgaa gaagactggg aagttcctgg tgatctcgga ggcaaacctg acggggtcgg
     9601 tatccggcga gatcgccgcc atcgtggcgt ccgaggcgtt cgagtggctg gatgctcccg
     9661 tctcgcggct cggggcgccg gatgtgccgg cggcggcgtt cgcccggccg atgatggact
     9721 acttcgtgcc ggacgccgaa aagatagagg ccgcgatgct ggagctggcc cgctactaga
     9781 agaggaacca ggagggactc gtggcgaaac cggtaaccat gccccggctt ggcgagagcg
     9841 tgacggaggg cacgatcatc cgctggctca aggccgaggg cgacgaggtg gcgatggacg
     9901 agccgctggc cgaggtggac accgacaagg tcaacgccga gctgccgtcg cccgtggccg
     9961 gcagggtgga gaagctcctc gtatccgagg gcgcgacggt ggacgtggga gtagagatcg
    10021 cgctggtcgc cgacgcagag agcgcgcccg cctcctccga cgccccaccg cgcgagaacg
    10081 tcgccgccgg agccccgacc gaggagttcc cggccgccgg cacggtctcc cagccggtgg
    10141 cgagcgggga gcgctcctcc gtggccgagg cgcggagcgt ggggagcggc ggcaatgggc
    10201 gcggcgggat cccggccgcc gagaccctgc gcctgacccg ctcctccccg gtggtgcggc
    10261 gtctcgccgc cgagcacggc gtagatatac aggagatccc cggctccggc accggcggcc
    10321 gggtgacgaa gaaagacatc gaaaggtaca tcgaggagcg cggcacgagg cgcgaggagt
    10381 acgcgccgcc gggcctgcgg gaggagcggg aagccttcgc gccacccgaa gctccgccgg
    10441 aacccgagcc gagccgcgag cgcgtaacgg tctacgaggg cgaccgggtc caggacctta
    10501 cgagcatccg ccgggcgata gcgaaccgca tgtccgcgag caagcgcgag gcgccgcacg
    10561 cctggacgat ggtcgaggtg gacgttacgg ggctcgccgc tctgcgcgag cgggagaagg
    10621 ctgccttcgc ggagcgcgag ggcgtgaagc tcacatacct gccgttcatc gtccaggctg
    10681 tggtcgagag tctcaaggag catccggtcc tcaactccgt ctgggacgga gacaggatcg
    10741 ttctgaggaa acgcatcaac atcggtgtcg ccgtaaacct cgaagacgcc ctcatagtcc
    10801 ccatcatccc cgacgccgat gaactgaaca tcgttggtct cgtccgcaag atagacgacg
    10861 ttgtccggcg tgccaggagc cggcagctcg gcccggagga catctccggc ggcaccttca
    10921 cggtcaacaa cccaggctct ctcggaagcg tggtctcgac gcccataatc aattacccgc
    10981 aggcggccat actctcggcg gaggccatcg tgaagcgccc ggtc
//
LOCUS       DASSMB010000064         3660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_901724, whole genome shotgun sequence.
ACCESSION   DASSMB010000064 DASSMB010000000
VERSION     DASSMB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901724"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VFJ72_04420"
     CDS             complement(<1..646)
                     /locus_tag="VFJ72_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HET7478746.1"
                     /translation="MSERPGSYESAGVSIDRGEKAVDLMRAAVEATHGSEVVSYPGGF
                     AGLYALAGYRDPVLASTIDGIGTKVLVAKALGRYDSLGADIVNHCANDVLATGARPMI
                     FLDYVASGRLDPETVAGIVRVAAEACKPLGVALIGGETAEMPGLYGEGDFEVVGVCVG
                     ACERDGVVTGERVCSGDVVLGLASSGLHTNGYTLARKLLADAGVSYAETPDLLDR"
     gene            complement(639..1112)
                     /locus_tag="VFJ72_04425"
     CDS             complement(639..1112)
                     /locus_tag="VFJ72_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478747.1"
                     /translation="MTGPLHIRRYEVGDHEDVLRLHEAGLREMGTFIEEPGFDLDLLD
                     IEGAYLEGDGDFLIGVCDEQVVAMGGLRKTSTGRAEVKRMRVDSAFRRRGFGQKILDA
                     LHRRAAALGYTTLHLDTGVNHGAARNLYEANGYRETRRGMVGPVDCVFYERDARV"
     gene            complement(1109..1543)
                     /locus_tag="VFJ72_04430"
     CDS             complement(1109..1543)
                     /locus_tag="VFJ72_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7478748.1"
                     /translation="MKDKAVTAAEIEAVLNWHEALNDGDTGRLIALSDPEIEVGGPRG
                     SGRGAQLLREWMERANIRLEPRRVFHRADTVVVEQEAEWRSADTGEVAGGQAGSSVFV
                     VRDGLVASVLRYDDLADALRVAALDESNEVDRSSSGEAAGSP"
     gene            complement(1540..2985)
                     /gene="purF"
                     /locus_tag="VFJ72_04435"
     CDS             complement(1540..2985)
                     /gene="purF"
                     /locus_tag="VFJ72_04435"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET7478749.1"
                     /translation="MDYRDTDGKPKEACGVFGLYSRELSGELAQRTYFGLYALQHRGQ
                     ESAGIAISDGERTTATRDMGLVSQVFDSTRLAALEAGHIALGHVRYSTTGSASWENAQ
                     PEFVGRGDVNVAVAHNGNLVNAEKLREELIQDGFQFNSTSDTTYIAAAAVRELEQGRS
                     CGEAVREAMHHLEGAYSVAMIFRDKLVAFRDPHGFRPLCIGEIEGGYAVSSETCGLDI
                     IGAKFLRDVEPGEVVVVDDDGLTSYRSESPRRALCVFEYVYFARPDSRFDGREVAGSR
                     HRMGELLAEEAPVEADVVIPVPDSGMMAAIGYSQRSGIPYAEGLIKNRYVGRTFIQPT
                     DGMRQLGIRLKLNPLPSVIEGKRVVVVDDSIVRGNTSRKLVELLYEAGAKEVHFRVSS
                     PPIKGPCYYGIDMDSPDQLVGANYTVEEIREQIGATTLAYLSVDAMVAATEQPKDRLC
                     RACFDGEYPITGTASKFALENRTHHAVARGG"
     gene            complement(3011..3373)
                     /locus_tag="VFJ72_04440"
     CDS             complement(3011..3373)
                     /locus_tag="VFJ72_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7478750.1"
                     /translation="MNVEKVNVAEKLALFEERWSPKVVGELNGQHVKLVKLLGEFVWH
                     HHEDEDELFMVVSGRFRMEFRDRSEWIEPGEFIVVPRGVEHRPVAEEEASVMLFEPAT
                     TVNTGSAGGALTAELERI"
     gene            complement(3370..>3660)
                     /locus_tag="VFJ72_04445"
     CDS             complement(3370..>3660)
                     /locus_tag="VFJ72_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase II"
                     /protein_id="HET7478751.1"
                     /translation="EMAVAGGIGAEVQLLPGGRQDVALFGETGGCILVAVPEGRIGEL
                     EEALSGVPHSRVGSTGGGRLKVAGVLDAGVDDMSAAYERDLFDVPVVPEEIG"
BASE COUNT          582 a         1395 c         1078 g          605 t
ORIGIN      
        1 gccggtccag aagatccggc gtctccgcgt aggagactcc ggcgtccgcg aggagcttgc
       61 gggccagcgt atagccgttc gtgtgcaagc cgctcgacgc cagccccagc accacgtcgc
      121 cggaacacac acgctccccg gtgacgaccc catctctctc gcacgcgccg acgcagacgc
      181 cgacgacctc gaagtctccc tctccgtaga gccccggcat ctcggccgtc tcgccgccga
      241 tgagcgcgac gccgaggggc ttgcaggctt cggcggcgac gcgaacgatc cccgcgacgg
      301 tctccgggtc gagccggccg ctcgccacgt agtccaggaa gatcatgggt cgcgcccccg
      361 tcgccagcac gtcgttcgcg cagtggttga cgatatccgc ccccaaagag tcatagcgcc
      421 ccaacgcctt cgcgacgagt accttcgtcc cgatcccgtc gatggtcgag gccaggacag
      481 ggtccctgta ccccgccagc gcatagaggc cggcgaaccc gcccgggtac gatacgacct
      541 cggaaccgtg cgtcgcctcg acggccgccc gcatgaggtc cacggccttc tcgccgcggt
      601 ctatggagac gccggccgac tcgtagctgc cgggcctctc agacacgcgc gtccctctcg
      661 tagaagacgc aatcgactgg ccccaccatg ccacgccgcg tctcccggta gccgttcgct
      721 tcgtataggt tccgcgcggc cccatgattt acgcccgtat ccagatgcag cgtcgtgtag
      781 cccagtgcgg ccgcccggcg gtggagcgca tccaggatct tctgcccgaa tcctcgccgc
      841 cgaaaggccg aatcgacgcg catacgcttg acctcagcgc gtcccgtcga tgtcttcctc
      901 agcccgccca tcgccacgac ctgttcatcg caaacgccga ttaggaagtc cccatcgcct
      961 tcgaggtacg caccttcgat atcgagcagg tcgagatcaa accccggctc ctcgatgaag
     1021 gtgcccatct cgcgcagccc ggcctcgtgc agccgcagca catcctcatg gtccccgacc
     1081 tcgtagcggc ggatgtggag gggtccggtc aagggctccc ggccgcctcg ccgctgctgc
     1141 tccggtcaac ctcattcgat tcgtcgaggg ccgccacgcg caaggcgtcc gcgaggtcat
     1201 cgtagcgcaa cacgctcgcc accagtccgt cacgcacgac aaagacggac gacccagcct
     1261 gaccgcctgc gacctctccc gtgtcggcgg agcgccattc cgcctcctgc tctactacca
     1321 ccgtgtcggc gcgatggaag acccgccggg gctccagacg gatgttcgcg cgctccatcc
     1381 actcgcgcag tagctgcgcg ccgcggccgg acccgcgcgg accgcccacc tcgatctccg
     1441 ggtccgacaa cgctatgagg cgcccggtgt ctccgtcgtt cagagcctcg tgccagttca
     1501 ggacagcctc gatctcggct gcggttacgg ccttgtcctt catccgcccc gcgcgacagc
     1561 gtggtgggtc cgattctcca gggcgaactt gctggcggtt ccggtgatcg ggtactcgcc
     1621 gtcgaagcag gcgcggcaga gccggtcctt cggctgctcg gtggcggcga ccatcgcgtc
     1681 caccgagagg taggcgaggg tggtcgcccc gatctgctcc cggatctcct cgaccgtgta
     1741 gttggccccg acgagttgat ccgggctgtc catgtctatc ccgtagtagc acggaccctt
     1801 gatcggcggc gacgagaccc ggaagtggac ctccttcgcc cccgcctcgt agagcaactc
     1861 gaccagcttg cggctcgtgt tcccccgcac gatggagtcg tccaccacga cgaccctctt
     1921 gccctcgatc acggacggca aagggttgag cttcagccgg atgccgagct ggcgcatccc
     1981 gtccgtcggc tggatgaacg tccggcccac gtagcggttc tttatgagcc cctcggcgta
     2041 cgggatgccg ctccgctgcg agtatccgat ggccgccatc atcccggagt cggggacggg
     2101 gatcaccacg tcggcctcga ccggcgcctc ctcggcgagc agctcgccca tgcgatgacg
     2161 cgaccccgct acttcccggc cgtcgaagcg cgagtcggga cgggcgaagt agacgtactc
     2221 gaagacgcac agggcgcggc gggggctctc ggagcggtag ctcgtcaggc cgtcgtcgtc
     2281 taccacgacg acctcgccgg gctccacgtc gcgcaggaac ttcgcgccga ttatatccag
     2341 gccgcaggtc tcgctggaga cggcgtagcc accctctatc tctccgatgc acagcggccg
     2401 gaagccgtgc gggtcgcgga aggcgacgag cttgtcccgg aagatcatgg ctaccgagta
     2461 cgccccttcg aggtggtgca tcgcctcccg gacggcctcc ccgcaggaac gcccctgctc
     2521 aagctcccgc accgccgccg ccgcgatgta cgtcgtgtcc gaggtcgagt tgaactggaa
     2581 gccgtcctgg atcaactcct ccctcagctt ctccgcgttg acgaggttgc cgttgtgggc
     2641 gaccgccacg ttcacgtcgc cgcgccccac gaactccggc tgggcgttct cccacgaggc
     2701 ggagccggtc gtcgagtagc ggacgtgacc gagcgcgatg tgtccggcct ccagcgcggc
     2761 gagccgggtg ctgtcgaaga cctgggagac gaggcccatg tcgcgcgtcg ccgtggtacg
     2821 ctccccgtcg gagatggcga tgcccgcgga ctcctggccc cggtgctgga gggcgtagag
     2881 tccgaagtac gtgcgctggg cgagttcccc ggagagctcg cgcgagtaga gcccgaagac
     2941 gccgcacgcc tccttcggct tgccgtccgt atctcggtaa tccacgctca aagcactcac
     3001 cttccgggtc ctagatcctc tccaactccg ccgtcaacgc gccacccgcg ctgcccgtat
     3061 ttaccgtcgt agcgggctcg aacaacatca cgctcgcctc ttcctccgcg accggccggt
     3121 gctccacccc acgcggcacc acgataaact cgcccggctc gatccactcg ctccggtccc
     3181 ggaactccat ccgaaaccgc ccgctcacca ccataaaaag ctcgtcctca tcttcgtgat
     3241 gatgccacac gaactcgccg aggagcttca ccagcttcac gtgctgcccg ttcagttcac
     3301 ccacgacctt cgggctccag cgctcctcga agagcgccag cttctccgca acgttcacct
     3361 tctctacgtt caaccgatct cctccggcac gacggggacg tcgaacaggt cccgctcgta
     3421 ggccgccgac atatcgtcca cacccgcgtc cagcactccg gcgaccttca aacgcccgcc
     3481 gcccgtactc ccgactctgg agtgcggcac accggacaga gcctcttcca gctccccgat
     3541 cctcccctcc gggaccgcga ccaggatgca accgcccgtc tccccgaaca gcgcaacatc
     3601 ctgccttcca cccggcagta gctgcacctc cgccccgata ccgcccgcga ccgccatctc
//
LOCUS       DASSMB010000065        10005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1282312, whole genome shotgun sequence.
ACCESSION   DASSMB010000065 DASSMB010000000
VERSION     DASSMB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282312"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFJ72_04450"
     CDS             complement(<1..197)
                     /locus_tag="VFJ72_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478752.1"
                     /translation="MENTGAHSPSTIPLWSWLALALLLLALFLLLSASGGLLAPVLGR
                     SASVFNYLHEFAHDGRHLLAVP"
     regulatory      complement(229..432)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            696..1235
                     /locus_tag="VFJ72_04455"
     CDS             696..1235
                     /locus_tag="VFJ72_04455"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET7478753.1"
                     /translation="MSDPNPPVSTRRGDDGTTTLLGAGRLSKSDVRIIILGDVDEASS
                     FLGLARAEAGSSEAGGDIADLLIGLQRLLYRVMGDVAMPGEENQVGESDLEYLEEALE
                     DWRGRTEIPKEFVIPGENRLGALLDVARSVVRRAERSLVAGGYAEEHPHALKAVNRLS
                     DLLFVVARNADGKSSLSKG"
     gene            complement(1359..1568)
                     /locus_tag="VFJ72_04460"
     CDS             complement(1359..1568)
                     /locus_tag="VFJ72_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478754.1"
                     /translation="MKKREEQERRRLEEEKDRIRSEYQRVLENSVAFERRIRDRWREK
                     NQVKNERIAELEAQVRELKRRLAED"
     gene            1993..2916
                     /locus_tag="VFJ72_04465"
     CDS             1993..2916
                     /locus_tag="VFJ72_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478755.1"
                     /translation="MNDVRSGRLLSPARDEAAQTGIGSCFGTFGELLQGTLPGQGQEF
                     LVTLPISRYSTARFTPLSGSREVYAYPSYKEKSRRLAETLIDIFDLDSGGFLSLQSEL
                     PDGKGCASSSADMVATALALQSAFGVFISRASLAEVMSSIEPSDGVMYRGVVSFYHRE
                     GVLRKFLGYLPPLTIAGLDEGGQVDTVEFNARPKPHGRVRRAEYEDLLYGIERAILRG
                     DLDCVGEISTRSAILNQEVLPKSHLDLLLDMRRRYRALGVVAAHSGTHLGLLLDPSSP
                     DHYQILPSIVSELERHSPNTCIHRTHDFQTA"
     gene            3180..3512
                     /locus_tag="VFJ72_04470"
     CDS             3180..3512
                     /locus_tag="VFJ72_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478756.1"
                     /translation="MILIGIVVYAAALAAFTLAGHEDTAPDLWLPIGALAGYAIGYFA
                     SRSPGHRGESEAYVLFFVALGALAIAAVIIVVSPPDPNSAPWMMILGFLMGVMAANYV
                     RLKYPPTR"
     gene            3583..3798
                     /locus_tag="VFJ72_04475"
     CDS             3583..3798
                     /locus_tag="VFJ72_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478757.1"
                     /translation="MAGETPEAWIGQEVLLHLWKSDGLGTEPCPAVLQGIDTFGVTVE
                     DPQQSRLTFYPWSTLREISPKKDPVSG"
     gene            complement(3884..4288)
                     /locus_tag="VFJ72_04480"
     CDS             complement(3884..4288)
                     /locus_tag="VFJ72_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HET7478758.1"
                     /translation="MTPNDEHQPAVRADEDDNPDRPAILVSLAREVDERLYRWVAVGA
                     EEEGIPCRLEPAEEAGEDDVAALAYTAARNSRLGVGIGVTADQVAVHERHMPAAQPVV
                     APGMEDDSARDTCRSAGSNAARLVIGVPLQLD"
     gene            complement(4285..6135)
                     /locus_tag="VFJ72_04485"
     CDS             complement(4285..6135)
                     /locus_tag="VFJ72_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HET7478759.1"
                     /translation="MSIIVGIDIGNSTTEAAVARIGDDGSPEFLSSGTTMTTGIKGTV
                     ENIPGIVHVLEEATEQAGIDIDDIDLVLLNEATPVIGDVAMETVTETIVTESSMVGHN
                     PSSPGGKGIGVGKTIHIRDLEDGERNGASPDGEVIVVVPQEYDFEVAADIIRGAMEAG
                     VEVKAGIVQGNDGVLIANRLPEPLPFVDEVAHVDKVPLDMLAAVEVAPQGHAVRQLSN
                     PYGIATLFSLSPEDTSHVIPIAKALVGLRSAVVIRTPAGDVQSKQIPAGSIKFMGEHQ
                     NLTVELERGADAIMDTLAQVQPLSDVQGEAGTNVGGMIENVRQTMADVTEKPLKDVNI
                     HDVLAVDCLVPQQVVGGIADEFSIETATGLAIMVDTDRLLMERVAETLAEKIGVRVEL
                     GGVEANTAILGALTTPGIDVPVAIIDGGAGSTDGAELQKGKVVEHVHLAGAGNMVTLL
                     IKNELGLERFDVAEQIKIYPLAKVESLFHIRHEDNGVQFFQEPLDPSLFARVVLVTDD
                     GDLIPIDSKHTLDRIRTVRQEAKRKVLLRNSIRVLERISPSGNIRFLDFVAIVGRFGL
                     DFELPQMLMNELSNYGIVVGVANVRGELGPTNAVATGLIINYCQRQDEAT"
     gene            complement(6222..6668)
                     /locus_tag="VFJ72_04490"
     CDS             complement(6222..6668)
                     /locus_tag="VFJ72_04490"
                     /inference="COORDINATES: protein motif:HMM:NF011972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HET7478760.1"
                     /translation="MAEKLHYEKDYPLAEKRPDLVKTAKGRALDEVTLDAVLSGEIEA
                     DEIRVTPEILEYQAQIADSVGRPQLAANLRRAGELTRVPDERILEIYNAMRPNMSTKE
                     ELHGIADELENEYDAKINADLVREAAGVYERRGRLKKPDEDEAEDN"
     gene            complement(6690..7277)
                     /locus_tag="VFJ72_04495"
     CDS             complement(6690..7277)
                     /locus_tag="VFJ72_04495"
                     /inference="COORDINATES: protein motif:HMM:NF011616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HET7478761.1"
                     /translation="MSDDAATREVVQDMELKELGEAKAGSSPDEVVIALSPAFGDQMQ
                     RQTLTKIPHAAILRELFAGIEEEEVKARVIRVWRSSDLAAIGYDAAKLSGSGISISIQ
                     SRGTTLIHQRDLVPLAPMELFPQAPSIDLSTYRHIARNAAQYAKGDHPSPVPQVNDPM
                     ARPKWQAIASVFHIRETEFVKPDEKPVELEVNFKK"
     gene            complement(7294..8997)
                     /locus_tag="VFJ72_04500"
     CDS             complement(7294..8997)
                     /locus_tag="VFJ72_04500"
                     /inference="COORDINATES: protein motif:HMM:NF011979.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HET7478762.1"
                     /translation="MLAEGQESGSKARTKRHKRFEALEQRDVNQDGFVKEWAEVGLIA
                     MGSPEDPEPSIKIEDGVIVEMDGVARDDFDLVDDFVARSAIDNTIAEEMMAKDAVEIA
                     RMIVDINVPRREVLKVFTGLTPAKILAVMENLNVVEMMMGLQKMRARRTPANQGHVTN
                     VRDNPVLMAADAAEAGLYGFAELETTVGMIRYAPFNALALLVGGQTGRGGVITQDAVE
                     EAMELQLGLRGLTTYAETVSVYGTEDAFRDGDDTPWSKALLASAYASRGIKMRFTSGT
                     GAEVQLASAQGKSMLYLEIKCVMLSKGVGVQGLQNGSISCIGVPAAVPGGIRAVLAEN
                     LVTTCLDLEAASGNDQSFSHSPFRRTARTLMQMLPGTDLIFSGYSTVPNYDNMFAGSN
                     FDCDDNDDYLIISRDLKVDGGIVPVDEQEVIAARNKAAQALRVVFEELGLPRITDEEI
                     EAATYAHGSKDMPERDTVQDTKAATKMMERGVTGMDVVKALGNRGFKDVAANVLEMQK
                     QRVSGDYLHTSAILNPDFTVLAAVNDLNDYEGPGTGYRLEGERWDKIADIRQALGPDD
                     I"
     gene            complement(9401..9769)
                     /locus_tag="VFJ72_04505"
     CDS             complement(9401..9769)
                     /locus_tag="VFJ72_04505"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HET7478763.1"
                     /translation="MRARILGNLVLGLHFVFILFSVLGGFLVLWKRWIAWLHVPSVLW
                     SSFVNLFSRVCPLTPLENRFRGLAGQSGYEGGFIEHYIAPLIYPGGMPRRMELIAGYS
                     VLIWNAFVYALVVFLRRRGD"
BASE COUNT         1802 a         3247 c         2966 g         1990 t
ORIGIN      
        1 ggaacggcga gcaggtgccg cccgtcatgg gcgaactcgt gcaggtagtt gaacacgctc
       61 gcgctccgtc ccagtaccgg cgccagaagc cccccgctgg ccgacagcag caagaacaaa
      121 gccaggagca gaagggctaa cgccagccaa ctccacaggg gtatggtgga cggtgaatgc
      181 gctccggtgt tctccatcag tgcctcctcc tcggggtcct cgccccagat aggtttacgg
      241 gcgagaggcc agtatcctga ctcccggctc ctcgctcccc cggccttccc gccgccggat
      301 ttctgtgtcg gcggtggctt atatcgggtt cgctccccgg ttacagtggc gggaccgtgc
      361 cggactcgca ccggcttcct ggactcctct tcccctactg acggacagca taccgacaac
      421 actcccgctc gtcaacggtc cgcaaaggtc caaacctccc ctgactcgca ggttccacgg
      481 gacagggcat cagctcaata ttcttcgaaa ggagccatgc caggccgatc cactctactc
      541 cactctactc aaaacctgga catcaccgag acagtacggc agatatcgcc tccgccaccg
      601 gagccgcttt agcttgcgca gcatgatgtt tcggactacg atgccatgga aggtgtagaa
      661 agggaaattt ttcgactcag gttaagggag tctcgatgag cgatcctaat ccgccggtct
      721 cgacccgccg cggtgacgac ggcaccacca ccctcctcgg tgcggggcgg ctgtccaaga
      781 gcgatgtccg catcatcatc ctgggcgacg tcgacgaggc cagctcgttc ctgggcctag
      841 cccgcgccga ggccggcagc tcagaggccg gaggcgatat agccgatctc ctgataggct
      901 tgcaacgcct gctttaccgg gtgatgggcg acgtcgccat gcccggtgag gagaatcagg
      961 taggagagtc ggaccttgag tacctggaag aagcgctcga agattggcgg gggcggacgg
     1021 agatcccaaa ggagttcgtc atcccgggtg agaacaggct cggcgccctg ctcgacgtcg
     1081 cccgctcggt ggtgcgccgc gctgaacgga gcctcgtcgc cggcggatac gcggaagagc
     1141 acccacacgc cctgaaggcc gtcaaccgcc tctcggacct gctcttcgtc gtcgcccgca
     1201 acgccgacgg caagtcttcg ctttcaaaag ggtaaggttc cgcgaagcac gtcctttccg
     1261 tgctcgacga catctgctcg caggctgtaa ttacagcacc ttacagcagg ccttcgtctc
     1321 ttccacggga acgcgcgcgg gcacggtaaa agtccgtctc agtcctccgc gaggcgccgt
     1381 tttaactccc tgacctgcgc ttcgagctcg gcgatcctct cgttcttcac ctggttcttt
     1441 tcccgccatc ggtcacggat gcgccgttcg aacgctacgg agttctccag aacccgctgg
     1501 tactcggacc ttatccggtc cttctcctcc tcaagcctcc ggcgttcctg ctcctctctc
     1561 ttcttcatgt ctctatcgtc ctactctagc gccgccagat caaatgtcag ctaaagacag
     1621 ggcatccatt atcaggagaa atccgctccg acctttttgg cgagcgaaat ttcagtcagt
     1681 acccgaagta acaatgtagc gcccggtcgc tggcggcgtt gaggaccccg ttactgtacg
     1741 ggcgcttgtg actcacccgc gtcggctcgc gaataccagg cttcgagtac gtcgagtgct
     1801 tccgcgataa gatgatgccc gacgtttgct acctcagcgc gacgaactat gaggagggta
     1861 caatggcgac cacggcggcg tccgggcagt cgggtcagga ccgctgcgct cgcgggtcac
     1921 gcacggtggg agcatgggga caagtagcat gggcaggatc ttcgaagttc ggaacagcgc
     1981 gcgagatagc gtatgaacga cgtccgtagc ggccggttat tgagcccggc gagagacgag
     2041 gccgcgcaga cgggcattgg gagttgcttc ggcacgttcg gcgagttgct ccagggaaca
     2101 cttccgggtc aggggcaaga attccttgtt accctgccga tctcccgtta ttcgaccgcc
     2161 aggttcacgc cattgtccgg gtcccgcgag gtctacgcat atccgtcata caaggagaaa
     2221 tcgcggcggc tggcagagac acttatagac atcttcgacc tggactcggg tggctttctg
     2281 tccctacaga gcgagttgcc ggacggtaaa ggttgcgcca gttcgtcggc cgacatggtc
     2341 gccacggccc tagctctcca gtccgccttt ggtgtcttca tctcccgggc gtctctggcc
     2401 gaggtcatga gcagcataga gccatcggac ggtgtgatgt accggggggt cgtctccttc
     2461 taccatcgcg aaggagtgct gaggaagttc ctgggttatc tgcctcccct gactatcgcc
     2521 ggtctcgacg aaggcggtca ggtagatacg gtcgagttca atgcgcgccc caaaccgcac
     2581 ggccgggtcc ggcgggcgga gtacgaggac ctgctgtatg gaatcgagcg ggctatctta
     2641 cggggcgacc ttgactgcgt tggagaaatt tctacccgca gcgccatcct gaaccaggaa
     2701 gtcctgccca agagccatct ggacctctta ctggatatgc gccggcggta cagagcgttg
     2761 ggtgtggtag ccgctcatag cggcacgcac ctgggcctgc tactcgaccc cagctctcca
     2821 gaccactacc agatcctgcc gtccatcgta tccgagttgg agcggcactc tcccaatacc
     2881 tgtatccacc gtactcacga cttccagacg gcgtaaccgg tgacgctgtt acgctcccgc
     2941 acctatcaag caatcaggag actacagtag cttgcggacc tgacgaacct cgccccatcc
     3001 aacgacccga tgagaggacc agaaatcgga gacgcggttc ctggcaggag tgtatgatac
     3061 tcgcgtggca gggaaaggcc catacacgac cggatggaca gcgagtagcc tggatccctg
     3121 gccgtctcgg gggtgagcgc atgagccgtc gcggacgtcc aaggaccagc cctggccgat
     3181 tgatactgat cgggatcgtc gtgtatgctg ccgctctcgc cgcctttact ctggcgggcc
     3241 acgaagacac ggccccggat ttgtggttgc ccataggcgc cctagccggc tatgccatcg
     3301 gttatttcgc ttcccggtct cccggacatc ggggcgagtc ggaggcttac gtcttgttct
     3361 tcgtggcgct cggcgcgctg gcgatagcgg cggtcatcat tgtggtgtcg ccgccggatc
     3421 caaactcggc tccgtggatg atgatcctgg gtttcctgat gggcgtcatg gccgctaact
     3481 acgtgaggct caagtatccg ccaacgcggt agcagatgac tccagccccc ataaccagac
     3541 cgaaattttg taagagccta ccaacaacta agggagaatt agatggcggg agagacaccc
     3601 gaagcctgga tcggacagga agtgctgtta catctctgga agagtgacgg tctcggcacc
     3661 gaaccctgtc cagcggtttt gcagggcata gatactttcg gtgtgacggt cgaagatccg
     3721 caacagagcc gtctgacctt ctacccgtgg agcaccctgc gagagatctc gcccaaaaaa
     3781 gacccggtta gcgggtgaaa cgaaagcgct tgaggcggac agagctctct tcttgaaagc
     3841 ggtctcgtga gcgccgagga aggccggttc gctgcctcta acgttagtcg agttgcaggg
     3901 gaacgcctat gaccagtcgc gccgcgttgg agccggccga ccggcacgta tctctagcgc
     3961 tatcgtcctc cattccggga gccacgaccg gctgcgcggc gggcatgtgc cgctcgtgga
     4021 cggcgacctg atcggcggtc acgcctatgc cgacgccgag gcgcgagttt cgagcagcgg
     4081 tataggcaag agcggcgaca tcgtcctcgc cagcctcttc agcgggctcc aggcggcagg
     4141 ggataccttc ttcctcagcc ccgacggcga cccacctgta caggcgctca tccacctcgc
     4201 gagccaggct cacgaggatc gccggccgat ccgggttgtc atcttcgtcc gcgcgaacgg
     4261 caggttggtg ttcgtcgttt ggagtcatgt tgcctcatct tgccgctggc agtagttgat
     4321 gatgaggccc gtggcgacag cgttcgtcgg cccgagttcc ccgcgcacgt tggcgacccc
     4381 gactactatg ccgtagttac tcaactcgtt catgagcatc tgcggcaact cgaagtcgag
     4441 cccgaagcgt ccgacgatag ccacgaagtc cagaaagcgg atattgcccg agggagatat
     4501 acgctccagg acccggatgc tgttgcggag gagcaccttg cgcttcgcct cctgtcgcac
     4561 cgtcctgatg cggtccagcg tgtgcttgct gtcgatcggg atgagatccc cgtcgtccgt
     4621 caccaggacc acccgcgcga agagactcgg atcgagcggt tcctggaaga actgcacccc
     4681 gttgtcctcg tggcgaatat ggaagaggct ctcgactttg gcgagcgggt agatcttgat
     4741 ctgctccgcc acgtcgaagc gctccaaccc caactcgttc ttgatgagca acgtcaccat
     4801 attcccggcc cccgccaggt ggacgtgctc cacgactttc cccttctgaa gctcggcccc
     4861 gtccgtcgag cccgccccac cgtcaatgat cgccaccggc acgtcgatcc ccggcgtggt
     4921 cagagcaccc agaatggccg tgttcgcctc gaccccgccc agctctacgc gcacaccgat
     4981 cttctcggcg agcgtctcgg caacccgctc catcaacaac cggtcggtgt ccaccatgat
     5041 cgcaagcccc gtcgcggtct cgatggagaa ctcgtcggcg ataccgccca caacctgctg
     5101 cggcaccagg caatccacgg ccagcacgtc gtggatattg acatctttca gcggcttctc
     5161 ggtcacatcc gccatcgtct ggcggacgtt ctcgatcatg cctcccacgt ttgtaccagc
     5221 ctcaccctga acgtcggata gaggttgaac ctgcgcgagg gtgtccatga tcgcgtccgc
     5281 cccgcgctcc agctcgacgg tgaggttctg atgctctccc atgaacttga tggagcccgc
     5341 ggggatctgc ttgctctgca cgtcaccggc gggagtgcgg atcacgaccg ccgagcggag
     5401 cccgaccagc gccttcgcta tcgggatcac atgcgatgta tcttccggtg agagtgagaa
     5461 cagcgtggct ataccgtaag ggttacttaa ctggcggacc gcgtgtccct gcggcgccac
     5521 ctcgacggcc gccagcatgt cgagcggcac cttgtccacg tgggcgacct cgtccacgaa
     5581 cggcaggggt tcggggaggc ggttcgcgat caggactccg tcgttaccct ggacgatacc
     5641 ggcctttacc tccacgcccg cttccatggc cccccggatg atatcggcgg ccacctcgaa
     5701 gtcgtattcc tgcgggacga cgactattac ctccccgtcc ggtgacgcgc cgttccgctc
     5761 gccgtcctcc aggtcgcgta tgtggatcgt ctttccgaca cctatgccct taccgcccgg
     5821 cgagctgggg ttgtggccca ccatcgaaga ctcggtgacg atcgtctcgg taacggtctc
     5881 catcgccacg tcgccgataa ccggggtcgc ctcgttcaag agcacgagat ctatatcgtc
     5941 aatatcgata ccggcctgct ccgtcgcctc ttccaggaca tggacgatac cgggaatatt
     6001 ctcaacggtg ccttttatcc cggtggtcat agtggtaccg gatgacagga actctggcga
     6061 accgtcgtcc ccgatcctgg cgacggcggc ctccgtggtc gagttgccga tgtctatgcc
     6121 gacgatgatg ctcatcgcac accgctctgc gaaactcgtt cggcggggga gccgtacacg
     6181 gctcccgtcc gccctctatt ttctttaatc actcaactct ctcagttgtc ctcggcctcg
     6241 tcttcatccg gttttttcag ccggccgcgc ctctcgtaca cgccggccgc ctcgcggacg
     6301 aggtccgcgt tgatcttcgc gtcgtactcg ttctcgagtt cgtcggcgat gccgtgcagt
     6361 tcttccttgg tggacatgtt cggccgcatg gcgttgtaga tctcgaggat gcgttcgtcg
     6421 gggactctcg tcagctcgcc cgcccgccgc aggttggcgg ccaactgggg acggccgacg
     6481 ctatcggcga tctgggcctg gtattccagg atctccgggg taacccggat ctcgtccgcc
     6541 tcgatctcgc cgctcagcac cgcgtcgagg gttacctcgt cgagagccct gcctttcgcc
     6601 gtcttgacca ggtccggccg cttttcggcc agggggtagt ctttctcgta gtggagtttc
     6661 tcggccacgt gaatctatct ccctttgagt tacttcttga agttgacttc cagctcgacc
     6721 ggcttctcgt ccggcttcac gaactcggtc tcccggatgt ggaacacgga tgcgatggcc
     6781 tgccatttgg gtcgggccat cgggtcgttc acctgcggga ccggcgaggg gtgatctcct
     6841 ttggcgtact gcgccgcgtt gcgggcgatg tgccggtagg tggacaggtc gatgctcggc
     6901 gcctggggga acaactccat cggcgccagc gggacgagat cccgctggtg gatcagggtg
     6961 gttccccgag actggatgga gatggagata cccgagccgc tcagcttggc ggcgtcgtag
     7021 ccgatagccg ccagatcgga ggaccgccag acgcggatca cgcgcgcctt tacctcttct
     7081 tcttcgatac cggcgaacag ctcgcgcaag atcgccgcgt ggggaatctt ggtgagcgtc
     7141 tgccgctgca tctggtcccc gaacgccggg gagagcgcga tgacgacctc gtcgggcgag
     7201 ctgccggcct tggcttcacc caactctttc aactccatat cctgcacgac ctctctggtc
     7261 gctgcatcat cggacatatg cctggccttt ctcttagata tcgtcgggac cgagcgcctg
     7321 gcggatatcg gcgatcttgt cccagcgctc gccttccagc cgatagcccg tgcccggtcc
     7381 ctcgtagtcg ttgaggtcgt tgaccgccgc gaggaccgtg aagtccgggt tcaggatggc
     7441 cgacgtatgc aggtagtcgc cggagacgcg ctgcttctgc atttcgagca cgttggcggc
     7501 gacgtccttg aacccgcggt tgcccagggc cttgacgaca tccattccgg tcacgccgcg
     7561 ctccatcatc ttcgtggccg ccttggtgtc ctggaccgtg tcgcgctccg gcatgtcctt
     7621 gctgccgtgg gcgtaggtcg ccgcctcgat ctcctcgtcc gtgatacgcg gcaggccaag
     7681 ctcctcgaag acgacccgca gcgcctgcgc cgccttgttg cgagccgcga tcacttcctg
     7741 ctcgtccacc ggcacgatgc ccccatctac cttcaggtcg cgcgagataa tgaggtagtc
     7801 gtcgttgtcg tcgcagtcga agttcgagcc ggcgaacatg ttgtcgtagt tcgggacggt
     7861 gctgtagccg gagaagatga ggtcggtgcc cggcagcatc tgcatcagcg tgcgggccgt
     7921 ccgccggaag ggcgagtggg agaacgactg gtcgttaccg gaggcggctt cgaggtccag
     7981 gcaggtcgtc accaggttct cggccagcac ggcccggatg ccgcccggca ccgcagcggg
     8041 gacgccgatg caactgatcg agccgttctg gagcccctga acgccgacgc ccttgctcag
     8101 catgacgcac ttgatctcca gatacagcat ggacttgccc tgggcggacg ccaactggac
     8161 ctccgcgccg gtccccgagg tgaaccgcat cttgatgccg cgcgaggcgt aggccgaggc
     8221 cagcagagcc ttcgaccacg gcgtatcgtc gccgtcccgg aacgcatcct cggtgccgta
     8281 gaccgagacg gtctcggcgt aggtcgtcag cccgcgcaag ccgagctgta gctccatcgc
     8341 ctcctcgacg gcgtcctggg tgatgactcc gccgcgtccc gtctggccgc cgacgagcag
     8401 cgccagggcg ttaaagggag cgtagcggat catgccgaca gtggtctcaa gctccgcgaa
     8461 accgtacaac ccggcttcgg cggcgtcggc ggccatcagg accgggttgt cgcgcacgtt
     8521 ggtgacgtgg ccctggttcg ccggcgtccg gcgcgcccgc atcttctgca ggcccatcat
     8581 catctcaacg acattcaggt tctccatcac ggccaggatc ttggccggcg tgagacccgt
     8641 gaagaccttc agcacttctc tacgcggcac gttgatgtca acgatcatgc gggcgatctc
     8701 gaccgcgtct ttcgccatca tctcttcggc gatggtgtta tctatagccg agcgcgccac
     8761 gaaatcgtcc acgaggtcga aatcgtcgcg ggccacgccg tccatctcga cgatcacgcc
     8821 gtcctcgatc ttgatgctgg gctccgggtc ctccgggctg cccatcgcga tcaggccgac
     8881 ctcggcccat tcctttacga agccgtcctg gtttacgtcc cgttgctcca gagcttcgaa
     8941 ccgcttgtgt ctcttggtcc gcgccttgct cccggattcc tgtccttcag cgagcacgtc
     9001 atttcctcct tctgcgatcc tgaaaaccgc ctattacaga cacaccaccg gttagatcca
     9061 tcctgctcag agagccttgc gagatgtgga attccccgat cacacggcag aggcctgttg
     9121 ccctttcccc aactcaagag ccgattcgaa tgagcccccc atcctgccta ttaccttcgc
     9181 ctatcctcac gcacttatag cagaaagctg atcgcagagt caagctggcg cccgtaccga
     9241 gacaacacgg ccacggcctg tgagacggcg cccgctgcga ggacggcaag cagccaaaga
     9301 catcgcccgg cgaaagcgga atgttattgc tctgaaccgg ctttgcacta atagtctgct
     9361 ctgaaccaag agccggcttt ggggctttag agccgcgtag ctaatcgcct cttcgccgca
     9421 ggaacaccac gagcgcatag acaaaagcgt tccagatgag aaccgaataa cctgctatca
     9481 actccatccg tctaggcatc ccgccggggt agatcaaagg agcgatgtag tgctcaataa
     9541 atcccccctc atagccagac tgccctgcga gaccgcgaaa cctgttctct aatggcgtga
     9601 gtggacagac acggctgaaa aggttcacga atgaggacca gagaaccgac ggaacgtgaa
     9661 gccaggcgat ccagcgcttc cagagcacaa ggaagccgcc caggaccgaa aagagaatga
     9721 agacgaagtg aagacccagg acaagatttc ccaggatgcg cgctctcact cactctcctt
     9781 ggcaattcgc tacagaggct cttactgtgc tgccgtctct tagcccaccg cgccgcgcaa
     9841 ggcctgctcc gggttctcta gtgacaggga acggcgagca ggtgccgccc gtcatgggcg
     9901 aactcgtgca ggtagttgaa cacgctcgcg ctccgtccca gtaccggcgc cagaagcccc
     9961 ccgctggccg acagcagcaa gaacaaagcc aggagcagaa gggct
//
LOCUS       DASSMB010000066         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1282338, whole genome shotgun sequence.
ACCESSION   DASSMB010000066 DASSMB010000000
VERSION     DASSMB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282338"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VFJ72_04510"
     CDS             complement(<1..197)
                     /locus_tag="VFJ72_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478764.1"
                     /translation="MENNGAHSPSTIPLWSWLVLALLLLALFLLLSASGGLLAPVLGR
                     SASVFNYLHEFAHDGRHLLAVP"
     gene            414..1082
                     /locus_tag="VFJ72_04515"
     CDS             414..1082
                     /locus_tag="VFJ72_04515"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7478765.1"
                     /translation="MEFVADFIRSIPNSPQGLLEFVMNLLLAHGYIVIFLGAALDNFG
                     LPASGDIVLFAGGYFANQGQAALPVVMLSGFAGALVSDNSVYWIGRVGGRPLLGQILK
                     IRFLHFLVDEKSLTKVEHYFETQGGRTVFLGRFGPGLRSMTPLFAGVSRMKYYQFFPY
                     NLAAGTFWAIAYSLVGYIFGEYWDDLLSVAKSVGFGFVGFVALLIALYVIRRRRRRKR
                     LDDE"
     gene            1144..2325
                     /locus_tag="VFJ72_04520"
     CDS             1144..2325
                     /locus_tag="VFJ72_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET7478766.1"
                     /translation="MPDSVQAGTTRDLQRLNHELSVINAIARELNRSVNLGDALDFTL
                     AQVAELLGLGTGWIWLIGDSSPQPYLAATLNLPPALANDPRRMDGSGYCYCLYTYKKG
                     DLSGAANVNVLTCSRLDGLVDGTGGLRYHASIPLYAGEKKLGVMNVAAPEWRGLSPED
                     LQLLYTVGDLLSIAVERARLFARSAEIGAVEERNRLARELHDTLAQGLTATALQLESA
                     DALLESGSQPEKARQRLRRALALTQTNLEEARRSVLDLRAAPLEGRSLSEALKTLVGR
                     WKSETGIAAIYKSVNPARPLPARVEVALYRVCGEALNNVARHADAGNVSVRLVSTPDH
                     VRLTVEDDGRGFDAAGISPDRHGLAGMRERAELLGGALEIRSAPGSGTRVTATVPLER
                     L"
     gene            2344..2997
                     /locus_tag="VFJ72_04525"
     CDS             2344..2997
                     /locus_tag="VFJ72_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7478767.1"
                     /translation="MLKILVADDHPMLREGLVAVLDTQEDFEVVGEASDGAEVVRLAE
                     ELRPDVILLDLEMPDTGGVTDGVAALQRLREAGSEAKAIVFTAYDTDERILGSLRAGA
                     RGYLLKGASRQEIFSAIRTVHAGGSLLQSDVTSKLLASMSRDERRAAHEPLTPRELEV
                     LALLARGRKNAEIAGNLFITERTVKFHVSSLMRKLGADNRTEAVGIAAQRGLIQLAD"
     gene            complement(3105..>3336)
                     /locus_tag="VFJ72_04530"
     CDS             complement(3105..>3336)
                     /locus_tag="VFJ72_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7478768.1"
                     /translation="AAIREGLATRFKGLPDVHYSDDRHWVSGNMGVSEWLLTGTTLDG
                     QRVRARGCDHWEFRDGKVVRKDSYWKIVEKPA"
BASE COUNT          570 a         1179 c         1008 g          579 t
ORIGIN      
        1 ggaacggcga gcaggtgccg cccgtcatgg gcgaactcgt gcaggtagtt gaacacgctc
       61 gcgctccgtc ccagtaccgg cgccagaagc cccccgctgg ccgacagcag caagaacaaa
      121 gccaggagca gaagggctaa caccagccaa ctccacaggg gtatggtgga cggtgaatgc
      181 gctccgttgt tctccatcag ttcctcctcc tcgcgctctc ccggccttcc cgccgccggg
      241 cttccgtgcc ggtggtagag ctctataccg ggttaccggg ccggactcgt accggcttcc
      301 tggactccct gcgtcccttt actatctgtg gcagcatagc ctcgcgcaag gtgcgcgtca
      361 acatggaggg ctggcatgag cgcgtgcgcc gcgtggtata aaggcgtctt ccaatggaat
      421 tcgttgcgga cttcatccga tccatcccga actctcccca gggtctgctg gagttcgtga
      481 tgaacctttt gctcgctcac ggctatatcg tcatcttcct cggggccgcg ctggataact
      541 tcgggctccc ggcatcgggg gacatcgtgc tgttcgccgg aggatacttc gccaaccagg
      601 gtcaggccgc tctaccagtc gtgatgctct ccgggttcgc cggagctctg gtctcggaca
      661 actcggtgta ctggatcggg cgcgtcggcg ggcggcccct gctaggccag atactcaaga
      721 tacggttcct acatttcctc gtagacgaga agagcctcac gaaggtggag cactacttcg
      781 agacccaggg cggacgcacg gtcttcctcg gacgcttcgg ccccggcctc cgcagcatga
      841 cccccctctt cgcgggcgtg agccgcatga agtactacca gttcttccca tacaaccttg
      901 ctgcagggac cttctgggcc atcgcctact ccctcgtcgg ctacatcttc ggcgagtact
      961 gggacgatct cctctccgtc gccaagtctg tcggcttcgg cttcgtcgga ttcgtcgccc
     1021 tgctcatcgc cctctacgtc atccggcggc gcaggcgccg caaacggctc gacgacgagt
     1081 agggcatcct cccgaatacc ccgctgataa actctcttgc ggatagagag gagcgcttac
     1141 gccatgcccg attccgtgca ggccggcaca acacgagatc tccagcggct caaccacgag
     1201 ctctcggtca tcaacgccat cgcccgcgag ttgaatcgct ccgtaaacct cggcgacgcg
     1261 ctcgacttca cgctcgctca ggtcgcggag ctactcggtc tggggacggg ttggatctgg
     1321 ctgatcggcg actcctcgcc gcaaccttac ctggccgcga ccctgaacct gccgcccgcc
     1381 ctggcgaacg acccgcgccg catggacggc tccggatact gctactgcct ctatacctac
     1441 aagaagggcg acctgagcgg cgccgcgaac gtcaacgtcc tcacctgcag ccgcctcgac
     1501 ggtctggtgg acgggaccgg cggcctccgc tatcacgcct cgatccccct ctacgccggt
     1561 gagaagaagc tcggcgtcat gaacgtcgcg gcccccgagt ggcgcggtct ctcccccgag
     1621 gatctccagc tcctctacac ggtcggcgac ctcctgagca tcgccgtcga acgcgcccgc
     1681 ctcttcgccc gcagcgcgga gatcggggct gtcgaggagc gtaaccgcct ggcgcgcgag
     1741 cttcacgaca ccctcgccca gggcctcacc gccacagctc tccagctaga atcggccgac
     1801 gccctcctcg aatccggctc gcagcccgaa aaggcccggc aacgcctgcg ccgcgccctc
     1861 gccctcacgc agaccaacct cgaagaggcc cgccgatccg tcctggacct gcgcgccgct
     1921 cccctggaag gacgctcgct ctccgaggcg ctcaagaccc ttgtggggcg ctggaagtcc
     1981 gagaccggta tcgccgctat ttacaagtcc gtcaacccgg cccggccgct cccggcgcgg
     2041 gtggaggtcg cgctctaccg cgtctgcggc gaggccctga acaacgtcgc ccgtcacgcg
     2101 gacgcgggga acgtatccgt gcgactcgtc tccaccccgg atcacgtccg gctcaccgtc
     2161 gaggacgacg gccgcggctt cgacgccgcc gggatctcac cggaccgcca cggcctcgcg
     2221 gggatgcgcg agcgcgcgga actgctcggc ggcgccctgg agatacggag tgctccgggc
     2281 agcggcaccc gcgtcacggc gaccgtcccg ctggagaggc tctagcgggt ggagaatgta
     2341 aagatgctaa agatactcgt cgccgacgac catccgatgc tgcgcgaggg tctcgtcgcc
     2401 gtcctcgaca cgcaagagga cttcgaggtc gtcggggagg cgtccgacgg cgcggaagtc
     2461 gtcaggctcg cggaagagtt acgtcccgac gtgatcctgc tggacctgga gatgccggat
     2521 accggaggtg ttaccgacgg cgtcgccgcg ctacagaggc tgcgcgaagc cgggtcggag
     2581 gcaaaggcca tcgtatttac cgcctacgac accgacgaac gcatcctggg ttcgctgcgc
     2641 gcgggggcgc gtggctacct gctcaagggc gcatcccggc aggagatctt ctccgccatc
     2701 cgtaccgtcc acgccggcgg ctcgctcctc cagtccgacg tgacgagcaa gctgctcgcg
     2761 agcatgagcc gggatgagcg gcgcgccgcg cacgaacccc tcacacccag agagctcgaa
     2821 gtgctcgcgc tcctcgcccg cggccggaag aacgcggaga tcgccggaaa cttgttcatc
     2881 accgagcgca ccgtcaagtt ccacgtcagc tccctcatgc gcaagctcgg cgccgataac
     2941 cgcaccgaag ccgtgggcat cgcggcgcag cgcggcctga tccaacttgc ggattagagc
     3001 ttgtttgatg acacggggaa gcggttaacg cctgaacctg gccgaagccg cgtaattttt
     3061 ctccgctagc gcgtcggctc taacccgcgg ttagagcgag agtgtcatgc cggcttctcg
     3121 acgatcttcc agtacgagtc cttccggaca accttcccgt ctcgaaactc ccagtgatca
     3181 catccgcgag cgcgaactcg ttgcccgtca agggtcgtac cggtcaggag ccactcggat
     3241 acgcccatat tgccactgac ccagtgtcgg tcgtcactgt agtgaacgtc aggaaggccc
     3301 ttgaagcgcg tcgctaaccc ctcgcgtatg gcagcc
//
LOCUS       DASSMB010000067        10956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_15780, whole genome shotgun sequence.
ACCESSION   DASSMB010000067 DASSMB010000000
VERSION     DASSMB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10956
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15780"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VFJ72_04535"
     CDS             complement(<1..1631)
                     /locus_tag="VFJ72_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET7478769.1"
                     /translation="MHSATTPSITASGADQGGRPIQNLLNMTPEARALFAEVAPAARE
                     ALSLLPARDADVFMLRLERYWQDLLDGLRQPYGARADYREFVTALISQLAASYRARSE
                     ELKLLDLERSLTPGWFQNERRIGYVFYTDRFAGDLRGIVPKLPYLKELGVNYAHLMPL
                     LKSRPGPNDGGYAVEDYRAVEPALGTMDDLREVCSALRAEGISVCLDLVLNHCAREHE
                     WAARARAGEEEYVAMFHTFPDRTVPDQYERTLPEVLPESAPGNFTWVEEMDRWVWTTF
                     SEYQWDLNWSNPRVFLEMTDVLLYLANQGVEVFRLDAVAFMWKRPGTDCQNQPEAHDL
                     LQALRACSRIVAPAVIHKAEAIVSPDNLIHYLGTHSRYGKESDLAYHNSLMVQIWSSL
                     ASRDTTLMTRVLMNFPEKPANTAWGTYIRCHDDIGWAVTEEDAALAGLDGPAHRAFLS
                     DYYSGDFSGSPARGAVYQLNPRTGDRRISGTLASLAGLESALETGDEALVEMSIRRIL
                     LGHAVILGYGGVPLLYMGDEIGLFNDFSYLEDPAK"
     gene            1666..2523
                     /gene="purU"
                     /locus_tag="VFJ72_04540"
     CDS             1666..2523
                     /gene="purU"
                     /locus_tag="VFJ72_04540"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HET7478770.1"
                     /translation="MSYNLRLLISCPDRKGLISAISSFIAMHDGNILSADQYVSGNGA
                     GTFFMRLEIEGEGFGLGRDEFDGAFAPLARRHNMDWRITHTDSPKRMAILVSRYDHCL
                     IDLLWRWDAGELEAEIPLVVSNHPELASRAEIYGIPFHHLPVTKENKAEQESRMLDLL
                     RENSIDVVVLARYMQILTPRFVQTYPDRIINIHHSFLPAFVGADPYRRAHERGVKTIG
                     ATAHYVTEDLDAGPIIHQDVAHVTHRDTVDDLVRLGREVERRVLARAVRWHLEDRILV
                     DGNRTVVFE"
     gene            2702..3832
                     /locus_tag="VFJ72_04545"
     CDS             2702..3832
                     /locus_tag="VFJ72_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478771.1"
                     /translation="MVRGCGSGQNANLSQSGDKTPKDSGAASGKTEETAKASKKQPKK
                     EKKPEQPFAAIPPPKGAEKTNPESSLNLAAQEDLNSGSAAQDQYASGETTAEQMEPEA
                     KLSVPRGQTLLKGKEDTSDGPLKKYRLVAYYGTPLSNLMGVLGESGPEEMMKQLKDQT
                     RAYSAADPDRPAVPTIELIASVAQRDPGADGLYVAQTPKAEIERYSRLAKENHALLLL
                     DVQLGRATVKQEIEILRPFLEKPYVHLAIDTEYSVEEGQVPGVDLGSVDGSEIQGAVE
                     YLDDLVRKKHLPDKMLLVHEFAQNIVTNKQSIKPTDNVEVVLHADGFGSPDDKALKYG
                     ILARDEPIQYGGFKVFYKQDRPVLKPEDVLTLDPAPAVVTYQ"
     gene            complement(3866..4501)
                     /locus_tag="VFJ72_04550"
     CDS             complement(3866..4501)
                     /locus_tag="VFJ72_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HET7478772.1"
                     /translation="MIEVWGRSNSVNVQKVLWCLGELELPFDRHDAGGLYGKNKEPEF
                     LSRNPVGLVPLVSDDGFDLWESNTIVRYLCAKYGEGGLWPEDPAARALSDKWMDYQLG
                     TVFPAFRETLLGLVRTPPEDRNPETIAASVKATAETLRVLDAHLEGGEYVAGETLTTG
                     DIALGSTVYRWLELEIDRPPLPNLEAWHDRLTRRPAYRDHVMVPFEMERIT"
     gene            4610..5857
                     /gene="hisZ"
                     /locus_tag="VFJ72_04555"
     CDS             4610..5857
                     /gene="hisZ"
                     /locus_tag="VFJ72_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HET7478773.1"
                     /translation="MQIQTHRKFSTTPGTRDVLPPESTRLLDVQQRIRERFRMHGFRE
                     VVTPALEYSEVIEEPKLRDSAFKLFDQDNQMLILRPEMTTPIARLIAQRLAGSPTPHK
                     LSYSLPVYRRASVGRGQSAEFHQAGVEVVGSTSPGEDAGAIALLVDVLETVGLRAPGD
                     FAVVLGQVAFYEGYLREVAPEAEPEIVAALVAKDLVRVDALAGNLPDGAAAGAREIPR
                     LVGPATDSGVLEEAARYADGPEATAALDNLREILAHLDAHGCLDAVILDLGLIGRRNY
                     YTGAVYEAYASGMGFSVANGGRYDNLLQRFGESLPATGFAISIERLISAMPEEPRPVC
                     LVLAGASAEASRTARRLRESGVPVLHVAEDLSEREATAYAHSVGAAWVSRPVPGGIDL
                     WPVEADGGSEVVPLEDVAGKVLA"
     gene            5854..6513
                     /gene="hisG"
                     /locus_tag="VFJ72_04560"
     CDS             5854..6513
                     /gene="hisG"
                     /locus_tag="VFJ72_04560"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET7478774.1"
                     /translation="MNGRPSGLRVAVPKGAIFEDALAALGSAGLPVEVLREDGRKLFY
                     EAAGTQFIVSRPSDVPVFVEYGAADVGIVGKDVLEEQEPNVMELRDLGTGACSMILAA
                     PASDAERVRAEIERAEVVRVATKFPRTARRYFEGLGRQAEIIELHGSIELAPLVGLAE
                     CIVDLTATGTTLRENDLVVMDEISYSTARLIANRGAYRLRNREVAALAATMQGVGGHV
                     G"
     gene            6506..7750
                     /gene="hisD"
                     /locus_tag="VFJ72_04565"
     CDS             6506..7750
                     /gene="hisD"
                     /locus_tag="VFJ72_04565"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET7478775.1"
                     /translation="MARTLDARDRNPEEFVRELRRPGGFLSPKVRDAARRIVEDVRER
                     GDAALIEYTERFDGVRPDPIRVSTEEIAAARESLSPEIEESFRVAIENVRAFHVREMD
                     RSWEISRNGATTGQRVRPLRRAGVYVPGGHGAYPSTMVMAAVPAQVAGVGSICVCAPP
                     GRDGRVSEWVLAVAGLLGLDEVHAVGGAQAISAMAYGTESVAAVNKIVGPGNDYVTAA
                     KLEVFGVVGVDAPQGPSEVVVIADASAFPERVARDLMAQAEHLSGASAILLTPSATLI
                     EEVEPLLTGEPADLITLVRVRDLAQAVEISNHYAPEHAHVISEDYEAIVQGLDAAGMV
                     AIGDFSPVALGDYAAGPSHALPTGGAASWASALGTHDFTARTNYIHFTQEALREIGPH
                     VERLARLEGFENHAESVGVRLK"
     gene            7789..8373
                     /gene="hisB"
                     /locus_tag="VFJ72_04570"
     CDS             7789..8373
                     /gene="hisB"
                     /locus_tag="VFJ72_04570"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET7478776.1"
                     /translation="MQRKGSAERKTGETFVRVSLDLDGTGVAEVSTGVGFLDHLLHLL
                     AHHSGMDLNVEAQGDTWVDDHHTVEDTGLVLGRAFDEALGDRNGIVRFADASAPLIES
                     LSTAVVDLGGRSHLTCNLGPMPEKIGTFDVELVQEFLRAFTQYGHFTLHFNCHYGENA
                     HHKVEAGIKALAVALREGMAPRSGGSASTKGVID"
     gene            8375..9028
                     /locus_tag="VFJ72_04575"
     CDS             8375..9028
                     /locus_tag="VFJ72_04575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7478777.1"
                     /translation="MADRGSGVRAVVFDLGGVLIDWDPRYLYRKIFAGEEEMEDFLTK
                     VATFEWHLEQDRGRTRKEGTALLLSQYPWYEQEIEAYYERWDEMFGGPIEETVGVLRG
                     LRERGLPLYALTNWSAEVFPLAREQYDFLAWFDDIVVSGEERMLKPEKEIYGVLVERT
                     GLDPATTVFIDDSPANVHAARELGFTGIEFRDADQLREDLERLEVLPTASKRPVPDA"
     gene            9021..9647
                     /gene="hisH"
                     /locus_tag="VFJ72_04580"
     CDS             9021..9647
                     /gene="hisH"
                     /locus_tag="VFJ72_04580"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET7478778.1"
                     /translation="MPETLNVAMVDYDAGNTLSVRRALEKVGANVDLTPDPDVVSNAD
                     AVVLPGVGAFGDCMRELRRRGMDEACLEAYRAGTPFLGVCVALQVLFEGSEESPGVDG
                     LSILPGNVVRFAANGLKVPHMGWNEVALSRSHPVLDGLDGEAFYFVHSYYPEPAEPAD
                     VLGTTDYGAEFCSAAGRENLIAVQFHAEKSSRAGLRLYENFLDWARNS"
     gene            9644..10369
                     /gene="hisA"
                     /locus_tag="VFJ72_04585"
     CDS             9644..10369
                     /gene="hisA"
                     /locus_tag="VFJ72_04585"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HET7478779.1"
                     /translation="MNSFTVYPAIDLRGGRCVRLKQGDFGRVREYDADPVSRAREWER
                     SGAAALHVVDLDGAERGRPVQLDLIGEIARSVAVPVQVGGGIRALEDLRAVMAAGASR
                     VVMGTAAVGDRELRLRAVEEAGDSLIVAVDARDGVVATHGWQRASGVDVLELSEELSR
                     DGVASVLYTDVARDGMDLGAALEKTAEVARLIPTIASGGVRGAGDIAALRKIEGVTGA
                     VVGTALYEGSVTLEELLAAAEAR"
     gene            10505..10903
                     /locus_tag="VFJ72_04590"
     CDS             10505..10903
                     /locus_tag="VFJ72_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478780.1"
                     /translation="MRRRTVLLLAAMLLAVMAAGGVALAATIDGTAADDTINGTRGSD
                     TIDGRGGDDLIRGFRGADSLTGGSGDDTINAGPLDEKAMDTVSGGGGNDRIRARNVPA
                     AKDVIDCGKGFDTVVVDSKDVFSGCNRVFR"
BASE COUNT         1879 a         3424 c         3878 g         1775 t
ORIGIN      
        1 tttttggccg ggtcttcgag gtaagagaag tcgttgaaca gcccgatctc atctcccatg
       61 tagagcaacg gcactccgcc gtagccgagg atgaccgcat ggccgagcag gatgcgccga
      121 atggacatct cgacgagcgc ttcgtctccg gtctccagcg ccgactccag gcccgcgagg
      181 ctcgccaggg tcccgctgat gcgccggtcg ccggtgcggg ggtttagctg gtagaccgcg
      241 ccccgcgcgg gactgccgga gaagtctccc gagtagtagt ccgagaggaa ggcccggtga
      301 gccggaccgt ccagcccggc gagcgccgcg tcctcctcgg tcacggccca gccgatgtcg
      361 tcgtggcagc ggatgtatgt cccccaggcg gtgttcgcgg gcttctccgg gaagttcatc
      421 aggacgcggg tcatcagcgt cgtgtcgcgg ctggcgaggg aggaccagat ctggaccatc
      481 aagctgttgt ggtacgcgag gtcggactcc ttgccgtagc ggctgtgcgt ccccaggtaa
      541 tggatcaggt tatccgggga gactatcgcc tcggccttgt ggatgaccgc tggggctacg
      601 atgcgcgagc aggcccgcaa tgcctggagg agatcgtgcg cttccggctg gttctggcag
      661 tcagtgccgg gccgcttcca cataaacgcc accgcgtcga gccggaagac ctccaccccc
      721 tggttcgcca ggtacagcag cacgtccgtc atctccagga agacgcgcgg gttcgaccag
      781 ttgaggtccc actggtactc gctgaaggtg gtccagaccc agcggtccat ctcctcgacc
      841 cacgtgaagt tgcccggtgc ggactccggc agcacctccg gcagcgtccg ctcgtactgg
      901 tccggcacgg tccggtccgg gaatgtgtgg aacatcgcca cgtactcctc ctctccggcg
      961 cgggctctcg cggcccattc gtgctcgcgg gcgcagtggt tcaggaccag gtccaggcag
     1021 acgcttatgc cctcggcgcg cagggccgag catacttcgc gcaggtcgtc catcgtgccc
     1081 agggccggct ccacggcccg atagtcctcc acggcgtacc cgccgtcgtt cggaccgggg
     1141 cgggacttga gtagcggcat caggtgcgcg tagttgacgc cgagttcctt gaggtacggg
     1201 agtttcggca cgatgccgcg caggtctccg gcgaaccggt cggtgtagaa gacgtagccg
     1261 atgcggcgct cgttctggaa ccagccgggt gtcaggctcc gttccaggtc cagaagcttc
     1321 aactcctcgg agcgggcgcg gtagctggcg gcgagctgtg agatcagggc cgtgacgaac
     1381 tcccgatagt ccgcgcgggc gccgtatggc tgccgcaagc cgtcgaggag gtcttgccag
     1441 tagcgctcca ggcgcagcat gaagacgtcc gcgtcgcggg cgggcaggag agagagcgcc
     1501 tccctcgccg ccggggcgac ctcggcgaag agcgcgcggg cctcgggggt catgttcagc
     1561 aggttctgga tgggtcttcc tccctgatct gcgccgcttg ctgttataga gggtgttgta
     1621 gcagaatgca aaacctggcg taacccttcc gcgaagcgag gcttgatgag ctacaacctg
     1681 aggctattga tctcctgccc cgacaggaag ggtcttatct ccgcgatctc atcgtttatc
     1741 gccatgcacg acggcaatat cctctccgcc gaccagtacg tgagcggcaa cggggcgggg
     1801 actttcttta tgcggctgga gatagagggc gaggggttcg ggctagggcg cgacgagttc
     1861 gacggagcgt tcgcgccgct ggcccgccgt cacaacatgg actggcgcat cacgcacacc
     1921 gactccccga aacggatggc gatcctggtc tcccgctacg accactgcct gatagacctg
     1981 ctctggcgct gggacgccgg agagctggag gccgagatac cgctcgtcgt ctcaaaccac
     2041 ccggaactcg cgtcgcgcgc ggagatctac ggcatcccct tccaccacct gccggtcacc
     2101 aaagagaaca aggccgagca ggagtcgagg atgcttgacc tgctccgcga gaactcgata
     2161 gacgtcgtgg tcctggcccg ctacatgcag atactgacgc ccaggtttgt ccagacctac
     2221 ccggaccgca tcatcaacat ccaccactcc ttcctcccgg ctttcgtcgg cgcggacccg
     2281 taccgccggg cgcacgagcg gggcgtgaag accatcggcg cgaccgccca ctacgtcacg
     2341 gaagacctcg acgccggtcc cataatccat caggacgtcg cccacgtcac gcaccgggac
     2401 acggtagacg acctcgtccg gctcggccgc gaggtggagc gccgggtact ggcccgcgct
     2461 gtccgctggc acctcgaaga ccgcatcctg gtggacggaa accgcaccgt cgtcttcgag
     2521 tagcttgcca gcgcgggggc cgcgctccgg ttaggataat gccttcggcg tttggaccag
     2581 ggaaggatgt cttctgacag accagtcgag attcgacagg aggaggggcg cgagacgaaa
     2641 gacgaggcgt ctggttatct ccgtcctgct gctcgctctg ctcctgatcg cggttctcgc
     2701 cgtggtgcga ggctgcggtt cggggcagaa cgcgaacctt tcgcagtcgg gagataagac
     2761 gccgaaggac agcggcgcag cgtccgggaa gaccgaggag accgcgaaag cttcgaagaa
     2821 gcaaccgaag aaagagaaga agccggagca gcctttcgcc gccataccgc cgccgaaggg
     2881 cgcggagaag acgaaccccg agagcagcct aaacctcgcc gcccaggaag acctcaacag
     2941 cggctcggcg gcgcaggacc agtacgccag tggtgagacc acggcagagc agatggagcc
     3001 cgaggcgaaa ctgagcgtgc cgcgcgggca gacgctcctg aagggcaagg aggacacctc
     3061 cgacgggccg ctcaagaagt accggctggt agcgtactac gggacgccgc tctcgaacct
     3121 gatgggcgtg ctcggggagt ctgggccgga ggagatgatg aagcaactca aggatcagac
     3181 gcgggcgtac tcggcggccg acccggaccg cccggcggtg ccgacgatag agttgatcgc
     3241 ttcggtggcg cagcgggacc ccggcgccga cggcctctac gttgcgcaga cgccgaaggc
     3301 ggagatcgaa cgctactctc gactcgcgaa ggagaaccac gctctcttac tgctcgacgt
     3361 gcagctcggg cgggcgacgg tgaagcagga gatagagatc ctgcgtccat ttttggagaa
     3421 accctacgtc cacctcgcca tagacaccga atactccgtc gaggagggac aggttcccgg
     3481 cgtggacctg gggtcggtgg acgggtcgga gatccagggc gccgtcgagt acctggacga
     3541 cctggtgagg aagaagcacc tgccggataa gatgctcctg gtacacgagt tcgcccagaa
     3601 catcgtgacg aacaagcaga gcatcaagcc cacggataac gtcgaggtcg tgctgcacgc
     3661 tgacggtttc ggttctccgg atgacaaggc tctcaagtac gggattctgg ctcgcgacga
     3721 gccgatacag tatggcggct tcaaggtctt ctacaagcag gaccggccgg tcttgaagcc
     3781 ggaggacgtg ctgaccctcg accccgcccc ggcggtcgtc acctaccagt agttcggaga
     3841 ttcgaatagt tgccgggtag cgcctttagg tgatcctttc catctcgaag gggaccatga
     3901 cgtgatcgcg gtatgccggc cgtctggtga gacggtcatg ccacgcctcc aggttcggga
     3961 gcggtgggcg atctatctca agttccagcc agcggtagac tgtcgacccg agcgcgatgt
     4021 ctccggtggt caacgtctcc ccggcgacgt actcaccgcc ttcgaggtgg gcgtcgagca
     4081 cccgcagggt ctcggccgtg gcttttacgg aagctgctat cgtctccggg ttccggtctt
     4141 cgggcggggt gcgaacgagg cccagtagag tctcccggaa tgcggggaag accgttccga
     4201 gctggtagtc catccatttg tccgagaggg cgcgcgccgc ggggtcttcg ggccagaggc
     4261 cgccttctcc gtacttcgcg cagaggtaac gcacgatggt gttcgactcc cagaggtcga
     4321 aaccgtcgtc cgacacgagg gggacgagcc ccacggggtt gcgggacaga aattccggct
     4381 ccttgttttt tccgtacagg ccgcccgcgt cgtgacggtc gaacggcaac tccagttcgc
     4441 ccaggcacca gagtaccttc tgcacgttca ccgagttgga cctgccccag acttcgatca
     4501 cccgcgtacc cctttactcg ctgacactag aggaagttta gccgggatcg atacacttgg
     4561 tcccgcgcgc tctaccaacg cccgcacatc ggttaagata gcggttcgta tgcagataca
     4621 gacgcacagg aagttctcga cgacgccggg cacgcgcgac gtgctgccgc cggagtccac
     4681 gcgcctcctg gacgtgcagc agaggatccg cgagcggttc cgtatgcacg gcttccggga
     4741 ggtggtgacg ccggcgctgg agtactcgga ggtcatagag gagccgaagc tcagagactc
     4801 ggcgttcaag ctcttcgacc aggacaacca gatgctcatc ctgcgccccg agatgaccac
     4861 cccgatcgcg cgcctcatcg ctcagcggct agcgggcagc ccgacgccgc acaagctctc
     4921 ctacagcctc cccgtctacc gccgcgccag cgtgggccgc ggccagagcg cggagttcca
     4981 ccaggccggg gtcgaggtcg tcggctccac gagccctggc gaggacgccg gagcgatagc
     5041 cttgctcgtt gacgtgctgg agaccgtggg cctgcgcgcc cccggcgact tcgcggtcgt
     5101 gctcgggcag gtcgcgttct acgagggcta cctgagggaa gtggcgccgg aggccgagcc
     5161 cgagatagtc gcggcgctcg tagcgaagga cctcgtgcgg gtggacgctc tggcggggaa
     5221 cctgccggat ggggccgcgg cgggcgcgcg cgagataccg cgcctggtcg gcccggcaac
     5281 ggatagcgga gtgctcgaag aggcggcccg gtacgcggat ggcccggaag ccacggctgc
     5341 tctggataac ctgcgcgaga tactcgccca cctcgacgcc cacggttgcc tggacgccgt
     5401 gatcctggac ctcggactca tcgggcggcg caactactac accggcgcgg tctacgaggc
     5461 gtacgcctcc gggatgggct tcagcgtcgc caacggcggc cgctacgata acctgctcca
     5521 gcgcttcggg gagtcgctcc ccgccacggg cttcgccatc tcgatagaac gtctcatctc
     5581 cgccatgccc gaagagcccc ggcccgtgtg cctcgtcctc gctggagcat cggcggaagc
     5641 atcccggaca gcccgccgcc tgcgcgagtc cggcgtgccc gtgctgcacg tcgccgagga
     5701 cctgtcggag agagaggcga cggcctacgc gcactccgtc ggggcggcgt gggtctctcg
     5761 tccggtcccc ggcgggatag acctctggcc ggtggaggcg gatggcgggt ccgaggtagt
     5821 gcctctggaa gacgtggctg gaaaggtgct cgcgtgaacg gccgaccgtc ggggctccgg
     5881 gtggcggtgc cgaagggcgc catcttcgag gatgcgctgg cggcgctggg gagcgcgggg
     5941 ctcccggtgg aggtgttgcg cgaggacggg cgcaagctct tctacgaggc ggcgggcacg
     6001 cagttcatcg tcagccggcc gtcggacgtg ccggtcttcg tggagtacgg ggcggcggac
     6061 gttgggatcg tggggaagga cgtgctggag gagcaggagc cgaacgtcat ggagcttcgg
     6121 gacctgggga cgggggcgtg cagcatgatc ctggccgctc ccgccagtga cgcggagcgg
     6181 gtgagggccg agatagagcg tgccgaggtc gtgcgggtgg cgaccaagtt cccgcgcacg
     6241 gcccgccggt acttcgaggg cctggggcgg caggcggaga tcatcgagct gcacggctcc
     6301 atagagctcg cgccgctcgt cgggctcgcg gagtgcatcg tggacctgac cgccaccgga
     6361 accacgctgc gcgagaacga cctggtggtg atggatgaga tctcctactc taccgcccgc
     6421 ttgatcgcaa accgcggcgc ataccggctc cgcaaccgcg aggtagccgc cctcgccgca
     6481 acgatgcagg gcgtgggagg tcacgttggc taggacgctc gacgcccgcg accgcaaccc
     6541 ggaggagttc gtccgggagc tgcgccgtcc gggtggtttc ctctcgccga aagtgcggga
     6601 cgcggcgcgg cggatcgtcg aggacgtgcg cgagagagga gacgccgccc tcatcgagta
     6661 caccgagcgc ttcgacgggg tgcggccgga ccccatccgg gtctccacgg aagagatagc
     6721 cgccgcgcgg gagtctctct ccccggagat agaggagagc ttccgcgtcg cgatagagaa
     6781 cgtgcgcgcc ttccatgtcc gggagatgga ccgttcgtgg gagatcagcc gcaacggcgc
     6841 cacgaccggg cagcgtgtcc ggccgctgcg gagggccggc gtctacgtcc ccggcgggca
     6901 cggcgcgtac ccgagcacga tggtcatggc cgcggtgccg gcgcaggtag ccggggtcgg
     6961 tagcatctgc gtgtgcgcgc ctcctggcag ggacggccgc gtgagcgagt gggtgctggc
     7021 ggtcgccgga ctgctcgggc tcgacgaggt acacgccgtc gggggggcgc aggcgatcag
     7081 cgcgatggcg tatggtacgg agagcgtcgc ggcggtcaac aagatcgtcg gtcccggcaa
     7141 cgactacgtg acggcggcga agctggaggt cttcggcgtc gtcggcgtgg acgcgccgca
     7201 gggaccgagc gaggtcgtcg tgatcgccga cgcgagcgct ttcccggagc gcgtcgcccg
     7261 cgacctgatg gcccaggccg agcacctctc cggcgcctca gccatcctgc tcaccccgag
     7321 cgcgaccctc atcgaggagg tcgagccgct gctcaccggc gagcccgccg atctaataac
     7381 cctggtccgg gtccgggacc tggcgcaggc tgtcgagatc tccaaccact acgccccgga
     7441 gcacgcgcac gttatctccg aggactacga agcaatcgtc cagggactcg acgccgccgg
     7501 gatggtcgcc atcggagact tcagcccggt ggctctcggg gattacgctg ccgggccgtc
     7561 gcacgcgctg cccacgggcg gcgcggcgtc gtgggcctcg gctctcggca cccacgactt
     7621 caccgcccgc accaactaca tccacttcac gcaggaggcg ctgcgggaga tcggaccgca
     7681 cgtggagcgc ctcgcgcgcc tcgaaggctt cgagaaccac gcggagagcg tcggggtgcg
     7741 gctcaagtag gcgggggcgg ttgttttctg atggaccaga ggaggagcat gcagcggaag
     7801 ggtagcgcgg agcggaagac gggggagacc ttcgtccggg tgagcctgga cctggacggc
     7861 acgggcgtgg cggaggtctc aacgggcgtg gggttcctgg accatctcct gcacctcctc
     7921 gctcaccact ccggcatgga cctcaatgtc gaggcccagg gcgatacgtg ggtggacgac
     7981 caccatacgg tcgaggacac ggggctggtg ctcggccgcg cgttcgatga ggcgctcgga
     8041 gacaggaacg ggatagtgcg ctttgctgat gcgagcgcgc cgctcatcga gtctctctcg
     8101 acggcggtgg tggacctcgg cgggcgcagc cacctcacct gcaacctcgg gccgatgccg
     8161 gagaagatcg ggacgttcga cgtggagctc gtgcaggagt tcctgcgcgc ctttacccag
     8221 tacggacact tcaccctcca cttcaactgc cactacggcg agaacgccca tcacaaggtc
     8281 gaggccggaa tcaaggcgct cgctgtggcg ctaagggagg gcatggctcc caggagcggc
     8341 ggcagcgcct ccacgaaagg agtgatcgat tagcttggct gaccggggca gcggcgtgcg
     8401 ggcggtcgtc ttcgacctgg ggggcgtatt gatcgactgg gaccccagat acctgtaccg
     8461 caagatcttc gccggcgagg aggagatgga ggacttcctg acaaaggtcg ccacgttcga
     8521 gtggcacctg gagcaggacc ggggacggac gcggaaggag gggactgcgc tccttctctc
     8581 ccaatacccg tggtatgagc aggagatcga agcgtactac gagcgatggg acgagatgtt
     8641 cggcggaccc atcgaagaga ccgtgggcgt gctgcggggc ctgcgcgagc gaggactccc
     8701 gctctacgcc ctcacgaact ggtctgcgga ggtcttcccg ctcgcacggg agcagtacga
     8761 tttcctggcg tggttcgatg acatagtcgt ctcgggagag gagcgcatgc taaagccgga
     8821 gaaagagata tacggcgtgc tcgtcgagcg taccggcctg gaccccgcca cgaccgtatt
     8881 catagacgac tcgcccgcga acgtccacgc tgccagggaa ctgggtttta ccggcataga
     8941 gttccgggac gcggatcaac tccgggaaga cctggagcgg ctggaagtcc tgcccactgc
     9001 ttccaagagg ccggtgcccg atgcctgaga ccctgaacgt ggcgatggtt gactacgacg
     9061 cggggaatac gctctcggtg aggcgcgcgc tggagaaggt cggggcgaat gtggacctca
     9121 cccctgaccc ggacgtggtc agtaacgcgg acgccgtggt gctccccggc gtcggggcgt
     9181 tcggagactg catgcgggag cttcgccggc gcgggatgga cgaggcgtgc ctcgaagcct
     9241 accgcgcggg cacgccgttc ctcggcgtct gcgtcgctct gcaggtactc ttcgagggtt
     9301 cggaggagtc gccaggagtc gatggactga gcatcttgcc aggaaacgtg gtccggttcg
     9361 cggcgaacgg cctgaaggtc ccgcacatgg gctggaacga ggtcgcgctc tcccggtccc
     9421 atcccgtact cgacggtctg gacggcgagg cgttctactt cgtccactcc tactatccgg
     9481 agccggccga gcccgcggac gtgctcggga ccaccgatta tggagcggag ttttgctccg
     9541 cggcggggag ggagaacctg atcgccgtgc agttccacgc cgagaagagc agccgcgccg
     9601 ggctcaggct ctacgagaac ttccttgact gggcgcgtaa ctcttgaaca gctttaccgt
     9661 ctacccggcc atagacctgc ggggcggtcg ttgcgtgcgg ctcaagcagg gggacttcgg
     9721 gcgtgttcgg gagtacgacg ccgaccccgt gtcgcgggcg cgggagtggg agaggagcgg
     9781 cgcggcggcg ctgcacgtgg ttgacctgga cggggcggag cggggacgcc cggtgcagtt
     9841 ggacctcatc ggcgagatag cccgctccgt cgccgtgccg gtgcaggtgg gcggcgggat
     9901 acgcgcgctg gaagacctgc gggccgttat ggcggcggga gcgagccgcg tggtcatggg
     9961 cacggcggct gtcggcgacc gggagctcag gctgcgggcc gtcgaggagg cgggcgattc
    10021 gctgatagtc gccgttgatg cgagagatgg tgtcgtggcg acgcacggct ggcagcgggc
    10081 gagcggggtg gacgttctgg agttgtccga ggagttgtcc agagatggcg tcgcttccgt
    10141 cctctatacc gatgtagccc gcgacgggat ggacctgggg gcggcgctcg aaaagaccgc
    10201 cgaggtggcc cgcctcatcc cgaccatcgc cagcggcggc gtgcggggcg ccggagacat
    10261 cgccgccctg agaaaaatag aaggcgtgac cggggccgtc gtcggcaccg cgctctacga
    10321 ggggagcgtt acgctggagg agttgctcgc ggcggccgaa gctcgctgag aacgagcgcc
    10381 gagccgttca ccgaaaaaat cactcatttg agtgatgcgc ggaggactcg aacgtgtata
    10441 tcatgctagc gttgcggtga ggagggccgg ggcgtcgcgt cctgtccccg agaaaggagt
    10501 aaggatgaga cggcggacgg ttcttttgct ggcggcgatg cttctagctg tcatggcggc
    10561 cggtggggtg gccctggcgg ctaccataga cggcacggcg gcggatgaca ccatcaacgg
    10621 tacgagaggc tcggatacca ttgacggccg tgggggcgac gacttgatcc gcggcttcag
    10681 gggagcggac tcccttacgg gaggctcggg cgacgatacg atcaatgccg gtccgctcga
    10741 tgagaaggcg atggacaccg tctccggggg tggcggtaac gaccggatcc gggctcgcaa
    10801 cgtcccggct gcgaaagacg tcatagactg cggtaagggc ttcgacacgg tcgtcgtgga
    10861 ctcgaaggac gttttctcgg gctgtaaccg cgttttccgc taacgacaat tacggttaag
    10921 gagtctggct aagaagtctc agcgccccgg cttacc
//
LOCUS       DASSMB010000068        21586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1662750, whole genome shotgun sequence.
ACCESSION   DASSMB010000068 DASSMB010000000
VERSION     DASSMB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 21586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21586
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1662750"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VFJ72_04595"
     CDS             complement(<1..1119)
                     /locus_tag="VFJ72_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET7478781.1"
                     /translation="MQRSEARSAARLNLDLKPPVRLDAWALAVAVGVVTGTVLPPLGI
                     ALVIASLVLAVGAIIFRDLVAFDWRLMAVLLPVFTAGGVGIAFLHLATPDPLAQLAAM
                     EPGEVILVGEISSPPARSSFGYRADLRVEHLWYEGEEVLRGGGVEVSAADLSVGVGDQ
                     VRVDGEISVPEPGEDGFDYAKYLSTKRISAVVDAYNVVPLSGEGWIGRVHRRTDVALG
                     YGLHPQEAAVVRGMVLGDRSRIPEELDEAFRSSGITHVLAISGQHVAILAAMIYFVLR
                     GVAMPIRACVPATLGLIWVYILIAGAPPSALRAGVVATLVLAARFFGRQVSPLHFMTT
                     MLAAVLAYNPVLIYSTGFQLSVAAVFGILLLRKPLKSFV"
     gene            complement(1106..1423)
                     /locus_tag="VFJ72_04600"
     CDS             complement(1106..1423)
                     /locus_tag="VFJ72_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7478782.1"
                     /translation="MSLLVVCVLIGGAAYASRLSESVPKVVYSASLEEVANDSQQPLK
                     VNINTADAESLDELPEVGPSTAEAIIEYRRANGLFRDVEDLEEVPGIGPATLEKIKPF
                     ATI"
     gene            complement(1571..1643)
                     /locus_tag="VFJ72_04605"
     tRNA            complement(1571..1643)
                     /locus_tag="VFJ72_04605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1608..1610),aa:Ala,seq:ggc)
     gene            complement(1710..2111)
                     /gene="rsfS"
                     /locus_tag="VFJ72_04610"
     CDS             complement(1710..2111)
                     /gene="rsfS"
                     /locus_tag="VFJ72_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7478783.1"
                     /translation="MSRDESAMRRVAPEAVEEGAISYEMAETAARAATEMFARDVVII
                     DLRESVSYTDYFIVASAETDRQTRRIAQEVIEKMIEAGHRPRSRRIDEGSAWISLDFI
                     DVVVHIFTDEARDYYRLESLWRSAPQEHWEE"
     gene            complement(2108..2755)
                     /gene="nadD"
                     /locus_tag="VFJ72_04615"
     CDS             complement(2108..2755)
                     /gene="nadD"
                     /locus_tag="VFJ72_04615"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET7478784.1"
                     /translation="MRIGIFGGTFDPVHLGHMVIAEQVADELGLDHVIFVPGGIPPHK
                     EASSVRATAEDRLAMVEAAVEGNERFSVDRVEVDAGRAMHTVETVPLLKQRSPDDDWY
                     FITGADEVSNLLTWKDPDRLLEEVVMVAATRPGYDLSALGHLESGLQNFDRIFAVECS
                     RLDISATGIRRRMLQGKSIRYLVPEGVHKVIEERGLYTGEGQRAEGGRLKGSEVL"
     gene            complement(2763..3995)
                     /gene="obgE"
                     /locus_tag="VFJ72_04620"
     CDS             complement(2763..3995)
                     /gene="obgE"
                     /locus_tag="VFJ72_04620"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET7478785.1"
                     /translation="MQFIDEARFVVRGGRGGDGSVSFNREKYKPRGGPDGGRGGDGGS
                     VIFRATEDLSTLEPYAYRKVIAAERGRHGSGNNRAGERGADVVLEVPVGTRIYDESGL
                     LEDLAEAGGSYVAARGGEGGRGNGSFATSTRQAPAFRERGLPGEEREVRLELRVLSDV
                     GLVGLPNAGKSSLLTRMSAARPRIGDYPFTTLTPQLGVVDEKTYPVPFVVADIPGLIS
                     GASEGRGLGNRFLRHVARARLLALVLDASEDPEGAEGTLLAELHAAGLSRKPRVVVLN
                     KIDLLDEELREYLAETFPGALQVSAETGEGVQELRDLLESRLAEMADEDDISDEEHRV
                     FRPTWKGMRVENDGGVYAVSGEELERLALKTDWQSPEGVENFQRQLERRGVVAALRRA
                     GAEPGDEVRIGEVVFDFR"
     gene            complement(4024..4299)
                     /gene="rpmA"
                     /locus_tag="VFJ72_04625"
     CDS             complement(4024..4299)
                     /gene="rpmA"
                     /locus_tag="VFJ72_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET7478786.1"
                     /translation="MAHKKGGGSSRNGRDTEGKRLGIKRYGGQVVTAGSIIVRQRGTK
                     LHPGQNVGKGSDDTLFAKTGGQVVFGRSKGRKVVSVLEAPVLEEATA"
     gene            complement(4303..4590)
                     /gene="rplU"
                     /locus_tag="VFJ72_04630"
     CDS             complement(4303..4590)
                     /gene="rplU"
                     /locus_tag="VFJ72_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET7478787.1"
                     /translation="MFAILNSGGKQYRVRQGEEILVDRVKGEAGDSVDLPVSFLVDDD
                     GADFEARTAKVEILEHVRGDKIQIYKYKPKKTYRKKTGHRQELSRIKVLEV"
     gene            complement(4695..5834)
                     /gene="rodA"
                     /locus_tag="VFJ72_04635"
     CDS             complement(4695..5834)
                     /gene="rodA"
                     /locus_tag="VFJ72_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HET7478788.1"
                     /translation="MSLTRASSRPVKTKTSEKWRSLDPVLLLVSLALSVFGIFAVYVA
                     GTDDRQAYAANQALGFAVGLAGAVPLAFVDYRVWQRYLRPIYVLIVAMLLAVILAGAA
                     AGGAQRWIDIGPVQVQPSEFAKLLVVVVLAGYIAESPIRENLTFLKSLGVLSIPAILV
                     FIQPDLGTALVFGAVFVTMTYIGGARLYQIGALGALAVLGAVVAFKADILQGYQKDRL
                     TVFIDPQGSGDVGYQVAQSKTAIGSGGLTGKGLDATTLANLGFLPEDHTDFIFSNLAE
                     RLGFIGGMVLLVLFLVLIWRILHIATISRDRFGVLIAVGVATIFLFHVFVNVGMTMGI
                     MPVTGIPLPFISYGRSSLVVSVLSLGLLQSIAMHSRAEYSKHPNV"
     gene            complement(5831..6334)
                     /gene="mreD"
                     /locus_tag="VFJ72_04640"
     CDS             complement(5831..6334)
                     /gene="mreD"
                     /locus_tag="VFJ72_04640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7478789.1"
                     /translation="MVHNSIIRAAVVVALAAVLDAVLSPYLSIGWISPKFTIVAVVLA
                     ASGLRDLQAVLLGFFGGILIDALGGGLFGVGAFGGLVAGTLAVRAGGLRRKDTERLVM
                     AQAVAISVAVYDLIQLAAINLAGLQGPGLGIYLVAGVVPDTLLNAVLAYLICGWMLRV
                     IRVKEGR"
     gene            complement(6360..7280)
                     /gene="mreC"
                     /locus_tag="VFJ72_04645"
     CDS             complement(6360..7280)
                     /gene="mreC"
                     /locus_tag="VFJ72_04645"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7478790.1"
                     /translation="MGRSKSSAASGLAALFILCIACLVLFTVYVKEGDCTDVKSGCGP
                     LHTVQLGAAEVLRPARSLTAAITKPFSETSKRIGGALNHDEEEKLQREARKNAELASQ
                     ASNLERENARLRELLKGQRSVYKYGPLARVIAPVGDQFTNRVTINVGADDGIKPEQPV
                     VVGNNTLVGRTTGQVSRHTAEVMLVTDQMFAAGVRILPPAQFDPSSGELTPAVTDENV
                     SYGEGMLRTNWEGYLGVDYVDLSSRAKKGDFVVTSGRAGDYELLFPPGLLIGKIESAS
                     AQDINQYKKIVVTPAARPDDLQEVRVIVDW"
     gene            complement(7290..8318)
                     /locus_tag="VFJ72_04650"
     CDS             complement(7290..8318)
                     /locus_tag="VFJ72_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7478791.1"
                     /translation="MFGGLFGRDVAIDLGTANTLVFVKGQGIVLSEPSVVAIDNKTDK
                     VVAVGSAAKRMLGRTPGNIIAMRPLKDGVIADFEVTEKMLSYFIRKVQPQRRIFRSLV
                     GPRVVVCVPSGVTGVELRAVKEATESAGARQAYTIEEPLAAAIGAGLPVNEAQGSMIV
                     DVGGGTSEVAVISLGGIVTKSSIRIAGDDVDDAITTYIQKEYKLAIGTQTAEQLKIEL
                     GSAFRLEEEESAEIRGRDLVTGLPKTIVITSEEVREAISVPVDAIVAAVRDTLDRTPP
                     ELASDIMDRGMVLAGGGALLRHMDERLRRETGIPVHVAEDALMCVALGSGRSLEEIDY
                     YRSALTSN"
     gene            complement(8370..9056)
                     /gene="radC"
                     /locus_tag="VFJ72_04655"
     CDS             complement(8370..9056)
                     /gene="radC"
                     /locus_tag="VFJ72_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7478792.1"
                     /translation="MERRRYTIKQLPPELRPRERLLSEGPEALSSAELLGIMFGIGSR
                     EKTAVELASEVISDSGDLFGLHNVSVHDLVKIHGVGEAKACIILAAVEFGKRLGRVRN
                     PGRPVISSPADVDGLLRGRIANRDRENFVAVLLNTKNEVLEISTVSIGTLSNSLVHPR
                     EVFKPAVRASAASVVLAHNHPSGRVEPSREDRQVTGRLVDAAEILGIEVLDHVIVGDG
                     YFSMKEHGML"
     gene            complement(9066..9686)
                     /locus_tag="VFJ72_04660"
     CDS             complement(9066..9686)
                     /locus_tag="VFJ72_04660"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7478793.1"
                     /translation="MLPASSSSSSSKLPDSRFILASESARRVDLLHQAGYDFETRKSG
                     FPEVTLEGPVATVLANARGKALAVAKIEPGRVVLAADTVVYRTDLPIDERIFGQAGDP
                     EEVRRMLSLLHGRVHEVYSGVAVTRGEQIIIRHSITSVRMREIPREEREAYALSGEGI
                     GKAGGYAIQGRAARFVEWIGGDYTNVVGLPLALTGRMLAGFGVRLY"
     gene            complement(9652..10050)
                     /gene="ndk"
                     /locus_tag="VFJ72_04665"
     CDS             complement(9652..10050)
                     /gene="ndk"
                     /locus_tag="VFJ72_04665"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET7478794.1"
                     /translation="MEQTLILVKGDGVRRRLIGEIIRRIEAKNLDIRGLKLMDVSREL
                     AEQHYAEHREKPFFGELVEFITSTPVVAMRVEGEGAIKVMRNLMGSTNPAEAAPGTIR
                     GDLALSMPDNLVHGSDSPESAARELKLFFE"
     gene            complement(10063..10566)
                     /locus_tag="VFJ72_04670"
     CDS             complement(10063..10566)
                     /locus_tag="VFJ72_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7478795.1"
                     /translation="MNAALQIPLLQQDTDPFKIIENFLTSPFLRIAGQLLLLLVACLW
                     IALIYWTYTDASRRGAMKIFWGIVAVIFPFLGTLIYLIVRPPEYLLDSRERELELAVL
                     EGELRNRVLLCPNCRNIVEKDYLVCPNCNWELKKSCIQCDKPLKMEWETCPYCGTNQR
                     SGKRINL"
     gene            complement(10654..11886)
                     /locus_tag="VFJ72_04675"
     CDS             complement(10654..11886)
                     /locus_tag="VFJ72_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET7478796.1"
                     /translation="MFHEVCEELDRRRHVTLGFERIERLLNLLGKPERGLRIVQVVGT
                     NGKGTTAVAIASALETLGFTSGAYLSPHVLSYTERVMIRGRFITEEDFAGAMGETIAL
                     ADANGVPASQFELLTAGAIKLFHDAGLDFAVLEAGLGARHDATSAAPAEAVVLTNVSL
                     DHTEYLGETVEEISREKLASLRPGATLFLGTGDPAVLNVARGVAASVKARTVELPPEE
                     ERRKPDRGAEAGLPPYAEGNVRLGVLVAENITGRDLGWEERERVARSVAGVLPARFEV
                     HEVRGVPVVVDGGHNAAGIEAALGGVRRLYGSRPLGLIFGVLRDKDAARMLAALAGEV
                     DLIVITRPLSDRAAEPEAVADELRRVRAGDPRSLVVTYAGDALEVAVEEMRRVDGVVL
                     VTGSLYTGAAVLGALREG"
     gene            complement(11906..14455)
                     /locus_tag="VFJ72_04680"
     CDS             complement(11906..14455)
                     /locus_tag="VFJ72_04680"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET7478797.1"
                     /translation="MSQAEIPKTYDPREVEERVYAEWERSGAFDADPNPEKEAFCIVM
                     PPPNVTGALHMGHALTFSIQDTFIRHARMKGYEALWLPGVDHASIALHNVVERKLMRE
                     EGKTRFDIGREEFVERCREFAAESRSQILGQIRRLGASADWRRLRYTMDEEYIDAVLT
                     AFVKLFNEGLIYKGNRITNWCPHDRSAISDLEVNYEEVNGKLYGLRYPFVDGKGPGPD
                     GRDYAQVFSTRPETMLGDVALVVNPDDERYTELVGRRVWVPFVDREIEVFADGYVDPS
                     FGTGVLKVTPAHDPNDFDIGQRLGLAPVNVLNPDGTINANGAKFEGMSREDARKAVVE
                     GLGAEGLLGEVKDYPHRVGHCDRCGTVIEPWLSEQWWVAMRELAEPAIEALQERKINV
                     YPDSWRRESIRWLENLQDWNVSRQLWWGHRIPVWYGPDGEVVASKESPGTGFEQDPDV
                     LDTWFSSGLWPFATLGWPEESEDLEYFYPTSLLATAREIMYLWVARMIMSGLRFRGEV
                     PFRKINVHSIVLAEDGTKMSKSKGNTINPLDLFDEYGTDAVRFGLLYQSSTQDFAYSY
                     ERASMGRAFVTKLWNATRFVLSYPEAEESGELSVSDRWILSEFNRAAREYDALLEECE
                     FSEAMRRIYDFAWHQFADWYIEIAKAVPSPATPGVLREVFTGILKLLHPVMPFVTEEM
                     YRVLGGKGMLIRQEFPAFDAALEDAEADRLLERTRRAVSAVRSFRAESRVEGELDGRL
                     PDGVEADVFQALAGVSPVQDMDGAFRAALPAGDVVVEVALTEELRRGEIERLRKEISR
                     VEGEVKRGQGKLSNEKFVERAPGHVVAGEREKLDVNTRMLDTLNQRLQDYL"
     gene            complement(14452..15723)
                     /gene="clpX"
                     /locus_tag="VFJ72_04685"
     CDS             complement(14452..15723)
                     /gene="clpX"
                     /locus_tag="VFJ72_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7478798.1"
                     /translation="MRDPSDQLQCSFCGKSQRQVRKLIAGPGVYICDECIELCNEIID
                     EEFSGPEVLKDDDLPKPREINRILNEYVIGQADAKKVLAVAVYNHYKRISMGPDSSDG
                     TELQKSNILLLGPTGSGKTLLAQTLARILNVPFAIADATALTEAGYVGEDVENILLKL
                     IQAADFDVKKAETGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKILEGTVASVP
                     PQGGRKHPHQDFLQIDTKNILFICGGAFGGLEEVIRQRIGKKSIGFGSDTTTRVEEEP
                     GAEFKHVQPEDLLKYGLIPEFVGRLPVISTLRSLEEKDLIRILTEPKNALVRQYRQFF
                     RYDKVDLNFTEDALKAVAELALERGTGARGLRSILETALLPTMYELPSRSDVSKCVVD
                     ADTVKEGVQPSLVTGSGSDYSYDEETPRTAS"
     gene            complement(15778..16380)
                     /locus_tag="VFJ72_04690"
     CDS             complement(15778..16380)
                     /locus_tag="VFJ72_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET7478799.1"
                     /translation="MSYYDPQGVIPYVIEQSPRGERAMDIYSRLLKDRIIFLGTPVDD
                     QVANAIMAQLLHLESEDPEQDINLYINSPGGSVTAGLAIYDTMQFVKPDISTTALGMA
                     ASMGAFLLAAGAKGKRNALPNTRILLHQPSVGGLAGQASDVEIHAKELILTKQRLNQI
                     LAENTGQEYDKIERDTDRDYIMGAEEGVEYGVIDNIVRQH"
     gene            complement(16377..17792)
                     /gene="tig"
                     /locus_tag="VFJ72_04695"
     CDS             complement(16377..17792)
                     /gene="tig"
                     /locus_tag="VFJ72_04695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET7478800.1"
                     /translation="MTANVTKLEDNKVRLEVEVSGEAVRKGVEAKVKELGRQVRVPGF
                     RPGKAPRRVIENHVGRDYIFMEALQEQLPNWYSSAVVETDLRPIDRPEIEFDDPMDEK
                     EGFKFSATVEVRPEAKLGEYTGLEVPKREVEVTDEQVEAQLEEMRGQFATLAAVEDRA
                     AQEGDFTIIDFRGERMATGEPLEGGDAEDYMLEIGRGELLEDFEKNLVGMNAGERKQF
                     GVTFPMDYAEESLRGQAVLFHVHLKEIKERDLPPLDDDFAKEASEFETLDELRSAVRE
                     QLEAAIEQRVEGEFRGRVLDAVAERAEVEIPEVMVHEKAHEMVESFERSIRAQGMEPE
                     QYYQLAGASHHDMEERVRPDAEDTVKKELVLDAVAAKEGIEADDEAVMHEIGHIAEGS
                     EQSPEQIAAMMRANGTFALLQEEMARQKALDYLVENAVAVEMPEEEEPAEEEPSEEGS
                     DEAVAVAEPGGETETSEGEQQ"
     gene            complement(17846..17927)
                     /locus_tag="VFJ72_04700"
     tRNA            complement(17846..17927)
                     /locus_tag="VFJ72_04700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17892..17894),aa:Leu,seq:tag)
     gene            complement(18062..18967)
                     /locus_tag="VFJ72_04705"
     CDS             complement(18062..18967)
                     /locus_tag="VFJ72_04705"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7478801.1"
                     /translation="MAAVLAVLIGAGAQVGGAPLARSLGVDRASDLNPLVNLKATHAS
                     FDKNGGSLLGGDPINILVLGVDDKPNDGSGSTARRSDTIMLVRISPQSGMIKLLSVPR
                     DLMVEVSPGVESKVNAAYSYGGVSQTVSALENYADIRIDRYAIVDFEGFEAVVDDIGG
                     VTVDSKGGFPPNVHIKEGVQHLNGRRALLYARYRGTPGGDLDRIERQQQLVAALRSKA
                     LRWNGVRKLPSVAATIEDHVQTDMGVQEAITLGRVLLQHGREARMTATQLKGTPETLP
                     DGEQVLRPDTQANQALLREFRYQAP"
     gene            complement(19331..20275)
                     /locus_tag="VFJ72_04710"
     CDS             complement(19331..20275)
                     /locus_tag="VFJ72_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP family channel protein"
                     /protein_id="HET7478802.1"
                     /translation="MSAVSTPARGGWRSTIWGELSAEFLGTFVLIAFGNGVVAMTVAA
                     LNQSGRGKEIFAASGDWLLIAFGWALAVTFAVYVAGGISGAHINPAVTLAFAVKRGFP
                     WSKVPGYMAAQMVGAFAGAALVYLNYHNAIASFENAKNITRGAADSTATFSIFATFPA
                     PYFNGSNIGPLIDQIIGTAFLVLIVFALVDVLNQPPKSNMAPLLVGLAVAAIGMSFGA
                     NAGYAINPARDLGPRVFAWLAGWGDVALPGVNYYFWVPIVGPLIGGVVGAFVYDLFIR
                     DVLTARGEHAEPDVEPRGRTVEEEVGAGNGTREPAAQG"
     gene            complement(21342..21414)
                     /locus_tag="VFJ72_04715"
     tRNA            complement(21342..21414)
                     /locus_tag="VFJ72_04715"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21379..21381),aa:His,seq:gtg)
     gene            complement(21447..21520)
                     /locus_tag="VFJ72_04720"
     tRNA            complement(21447..21520)
                     /locus_tag="VFJ72_04720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21484..21486),aa:Arg,seq:tct)
BASE COUNT         3937 a         7388 c         6205 g         4056 t
ORIGIN      
        1 cacaaaggac ttcagaggtt tcctcaacag gaggatgccg aagacggcgg cgacggagag
       61 ctggaagccg gtgctataga tcagtaccgg gttgtaggcg agcacggcgg ccagcatcgt
      121 cgtcatgaag tgcagcggcg agacctggcg tccgaaaaag cgggcggcga gaaccagcgt
      181 ggccacgacc ccggcccgga gcgccgacgg cggcgcgccc gcgattagga tatagaccca
      241 gatcaggccg agcgtggctg ggacgcacgc ccttatgggc atcgcgaccc ctcgcaggac
      301 gaagtagatc atggccgcca gtatggcgac gtgctggccg ctgatcgcca ggacgtgagt
      361 tatgccggag gatctgaacg cctcgtcaag ctcctccggg atgcgcgacc gatcacctag
      421 aaccatgccc cgcacgactg ccgcctcctg cggatgaaga ccgtacccga gagccacatc
      481 cgtccggcgg tggacgcggc cgatccagcc ctctccgctc aacggcacga cgttgtaagc
      541 gtccacgaca gcggagatcc gtttggtaga cagatatttg gcgtaatcga agccgtcttc
      601 gcctggctcc gggaccgata tctctccgtc tacgcggacc tgatctccca cgccgacgct
      661 caggtctgcg gcggagactt ccactccgcc gccgcgaagg acctcctcac cctcgtacca
      721 caggtgctcg acccgcaggt ccgcccggta gccaaagctg ctgcgcgccg gtggagagga
      781 gatctcaccg accaggatga cctctccagg ctccatcgcg gcaagctgcg ccagcgggtc
      841 cggggtcgcg agatgcagga acgcgatgcc aaccccgcca gccgtgaaga ccggcaaaag
      901 caccgccatc agccgccaat cgaaagctac caagtcccta aatatgatcg ctccgacggc
      961 aagaaccaga ctggcgatga ccagcgctat acccagcggc gggagcaccg tgcccgtcac
     1021 gacgcccacg gccacggcta gagcccaggc gtccagacga accggcggct tgagatccag
     1081 gttcagccgt gccgccgagc gggcctcaga tcgttgcaaa gggtttgatc ttctccaggg
     1141 tagccggccc aatgccgggc acttcctcca gatcctcgac gtctctgaag agtccgttcg
     1201 cccgccggta ctctatgatc gcctccgcgg tggacggccc tacctcgggc agctcgtcca
     1261 aactttcagc gtccgcggtg tttatgttga ccttgagcgg ctgctggctg tcgttggcga
     1321 cctcttcgag cgacgcgcta tacacgactt tcgggacgct ctccgagagg cgggaagcgt
     1381 atgcggcgcc gccgatcagc acgcagacaa ccagcaggct cacgacgatc tgtggcgtgt
     1441 aagacctgaa aaaagacgtg agagggctgt cggatgcacg acccctgcgc gttgtggtgt
     1501 cgaaaccttc cctcatagcg ccgatgttcc gcagacccgg cgtccccaaa cctgtccgac
     1561 cctgcgaaag tggagccgag ggggatcgaa cccctgacct ccgcgctgcc agcgcggcgc
     1621 tctcccagct gagctacggc cccaaaaaga ccttcagagt atagataccg tcccccgaag
     1681 tgtcaacgcg gggacgtaga gtctatgaac tactcctccc agtgctcctg aggggcgctg
     1741 cgccagaggg attcgagacg atagtaatcg cgggcctcgt cggtaaagat gtggacgacc
     1801 acgtcaatga agtccaggct gatccaggcg ctcccctcgt cgattcgccg ggagcgcgga
     1861 cgatggccgg cctcgatcat cttctcgatg acctcctggg ctatccgccg ggtctggcgg
     1921 tcggtctcgg cgctcgccac gatgaagtag tccgtgtagg agacggattc ccgcaggtct
     1981 atgatcacta cgtccctggc gaacatctcg gtcgccgccc gtgccgcggt ctcggccatc
     2041 tcataagaga ttgcgccttc ttccaccgcc tccggcgcca cacgccgcat ggccgactcg
     2101 tcgcggctca cagtacctca ctacccttca agcgtcctcc ttctgcgcgc tgtccctctc
     2161 ctgtatacaa acctctctcc tctatgactt tgtgcacccc ttccggtacc aggtatctaa
     2221 tgctcttgcc ctgcaacatc ctgcgccgga tgccggtcgc cgagatgtcc agccgggagc
     2281 actctaccgc gaagatccgg tcgaagttct gtagaccgct ctccaggtgg ccgagcgccg
     2341 agagatcgta gccgggccgc gtcgccgcca ccatgactac ttcctccagc agcctgtcgg
     2401 ggtccttcca tgtgagcagg ttggatacct cgtccgcgcc ggttatgaag taccaatcgt
     2461 cgtccggtga gcgctgcttg agtagcggca ccgtctcgac ggtgtgcatg gctctcccgg
     2521 catccacctc cacgcggtcc acggagaacc gctcgttccc ctcgactgcg gcctccacca
     2581 tcgccagccg gtcctcggcc gtggcccgga cactggatgc ctccttgtgc gggggtatcc
     2641 ctcccggcac gaagatgacg tgatccagac ccaactcgtc cgcgacttgc tcggcgatga
     2701 ccatgtgccc cagatgcacg gggtcgaagg ttcctccgaa gatccctatc ctcatatctc
     2761 cctcaccgga aatcgaacac cacctcaccg atccggacct cgtcgcccgg ttcggccccc
     2821 gcccgccgca acgccgccac gacgccgcgt ctctctagct ggcgctggaa gttctcgaca
     2881 ccctccggac tctgccagtc cgtcttgagc gcgagacgct ccaattcctc gcccgatacc
     2941 gcgtacacac cgccgtcgtt ctccacccgc atgcctttcc acgtcggccg aaagacgcga
     3001 tgttcctcgt cgctgatgtc gtcttcatca gccatctccg ccagccggga ttcaaggaga
     3061 tcccgcaact cctggacccc ctctccggtt tcggcggaga cttgtaaagc accagggaac
     3121 gtctctgcga gatactcccg caattcctcg tccaggagat ctatcttgtt caacacgacc
     3181 actcgcggtt tgcgggagag gccagccgcg tgcagctccg ccagcaacgt accctcggcc
     3241 ccttcggggt cctcgctcgc atctaaaact agcgcgagca gccgcgcccg cgcgacgtgg
     3301 cgcaggaagc ggttcccgag cccgcggccc tcgctggccc cggagattag tcccggtatg
     3361 tccgctacca cgaacgggac cgggtacgtc ttctcgtcta cgaccccgag ctgcggcgtc
     3421 aacgtggtga acggatagtc cccgatcctg ggccgggcag cgctcatccg ggtgaggaga
     3481 gaggacttgc cggcgttcgg gagacccacc agcccaacgt ccgagagcac tcgcaactcc
     3541 agccggacct cgcgctcctc ccccggcaga cccctctcgc ggaaggcggg agcctggcgg
     3601 gtggacgtgg cgaaggagcc gttgcccctg cctccctcgc cgccgcgtgc agctacataa
     3661 gagcctccgg cctcggccag atcctccagc agcccactct catcgtagat gcgcgtgccg
     3721 accggcactt cgagcaccac atcggcgcct cgctcgccag cgcggttatt accggacccg
     3781 tgacggccgc gctcggccgc gatcaccttg cggtaagcgt agggttcgag ggtcgatagg
     3841 tcttcggtgg cgcgaaagat cacggacccg ccgtcaccgc cgcggccgcc gtccggaccg
     3901 ccacgcggct tgtacttctc gcggttgaaa gagacgctgc cgtcaccgcc gcggcccccg
     3961 cggacgacga accgggcttc atcaatgaac tgcaacgggg ctagataccc ccgtaacgcg
     4021 gtctcaggcc gtggcctcct cgagtacagg cgcttcgagc acgctgacta ccttgcggcc
     4081 cttgctccgg ccgaacacca cctgaccgcc ggtcttggcg aagagggtgt cgtccgagcc
     4141 cttgccgacg ttctgaccgg gatggagctt cgtccctctc tggcgcacta ttatgctacc
     4201 ggcggtaacg acctggcccc cgtaacgctt tatgccgagg cgcttgccct cggtatccct
     4261 gccgttcctc gacgagccgc cgcccttttt atgagccatt ccctatacct ccaaaacctt
     4321 tatccgggac agctcctgcc ggtgcccggt cttcttgcga tacgtcttct tgggcttgta
     4381 cttgtaaatc tggatcttgt cgccccgaac gtgctccagt atctcgacct tcgccgtccg
     4441 ggcctcgaag tccgccccat cgtcgtcgac caggaagcta accggcagat ccaccgagtc
     4501 cccagcctca cccttgaccc ggtccacgag gatctcctcg ccctggcgca cccggtattg
     4561 ctttccaccg ctattcaata tcgcaaacac ttacgtctct cctgctatct tggggatcct
     4621 ggcagaggcg cgatacgccc tctacagccg cctagtatac ccgccggacc cactatcggc
     4681 cagtttgcat catattatac atttgggtgt ttagaatact cagcccgcga gtgcatggct
     4741 atgctctgga gcaggcccaa cgataggacg ctgaccacga ggctacttcg tccgtagctg
     4801 atgaacggca acgggatgcc ggttactggc atgatcccca tcgtcatccc gacgttcacg
     4861 aaaacgtgga acaggaagat ggtcgccact cccacggcga tcagcactcc gaagcggtcg
     4921 cgggagatcg tggcgatgtg caggatgcgc cagatcagga ccaggaacag caccagcagc
     4981 accatcccgc cgatgaaccc gagccgctcg gcgaggttcg agaagataaa gtcggtatgg
     5041 tcctccggca ggaagccgag gttggcgagc gtcgtggcgt cgagaccttt cccggtgagg
     5101 ccgccggacc ctatcgcggt cttggactga gccacctggt agccaacatc tccgctaccc
     5161 tgaggatcta tgaagacggt gaggcgatcc ttctggtatc cctggagtat gtccgccttg
     5221 aaagcgacga ccgcgccgag cacagccagc gcgcccaggg ctccaatctg atacagccgc
     5281 gccccgccaa tataggtcat agtcacgaac acagccccga agactagcgc agtccccagg
     5341 tccggctgga taaagaccaa tatcgccggg atagagagaa cacctagaga cttcaggaag
     5401 gtcaggttct cccgaatcgg gctctccgct atgtaaccgg ccaacactac aaccacgagt
     5461 agcttcgcga actccgaggg ctgtacctgc acgggaccta tatcgatcca gcgctgagcg
     5521 cctccggcgg cggcgcccgc caggatcacg gcgagcagca tcgctactat gaggacgtaa
     5581 atcggccgca ggtaacgctg ccacacacga tagtccacga acgcgagcgg gacggccccg
     5641 gcgaggccaa ccgcgaaccc gagcgcctgg ttggcggcgt acgcctgacg gtcatcggtc
     5701 cccgcgacat acaccgcgaa gatgccgaac accgagaggg ccagagagac cagcaagagg
     5761 acgggatcca gcgacctcca cttctctgag gtcttcgttt tcacgggcct gctacttgcg
     5821 cgggtaaggc tcatctacct tcctttacac ggatcacacg gagcatccag ccgcagatca
     5881 gataggctaa cacggcgttg agcagcgtgt cggggacgac gcccgctaca agatagatgc
     5941 cgagtcccgg cccctggaga ccagccaggt ttatcgccgc gagctggatc agatcgtaca
     6001 ccgccacgga gatggcgacg gcctgggcca ttacgagacg ttcggtgtcc ttgcgacgca
     6061 gacctccggc gcgcacggcg agagtcccgg cgaccagccc gccgaatgcc cctaccccaa
     6121 acagccctcc cccgagagcg tctataagga tgccgccgaa aaatcccagc agcacagcct
     6181 gcaagtccct gagcccggag gcggcgagca ccacggcgac gatcgtgaac ttcggagaga
     6241 tccaaccgat actgagatac ggtgatagca cggcgtccag gaccgcggcg agcgccacca
     6301 ctaccgcggc gcgaataatc gagttatgta ccaacttcta ccgccggact actctcgggt
     6361 taccaatcaa cgatcacccg cacttcctgg aggtcgtccg ggcgcgcggc cggcgtaacg
     6421 actatcttct tgtactggtt gatgtcttgc gccgatgcgg actcgatctt gcctatgagc
     6481 agaccgggcg ggaagagaag ctcgtaatcc cctgcgcggc cgctggtgac gacgaagtcg
     6541 cccttcttcg cccgcgagct gaggtccaca tagtctacgc cgaggtagcc ctcccagttc
     6601 gtccgcagca taccctcccc gtaggagacg ttctcgtcgg tgacggccgg ggtcaactcg
     6661 ccggaggagg ggtcgaactg cgcgggcggc agtatccgca cccccgccgc gaacatctgg
     6721 tccgtgacga gcatcacctc ggcggtatgc cgggagacct gcccggtagt acgcccgacc
     6781 agggtattgt tgcccacgac aacaggctgt tccggcttta tcccatcgtc cgctccgaca
     6841 ttgatcgtga cccggttggt gaactggtct ccgacgggcg ctatgacgcg ggccagaggt
     6901 ccgtacttgt atacgctgcg ctgccccttg agcaactctc gcaggcgcgc gttctcacgt
     6961 tcgaggttcg aagcctgaga tgccagctcc gcgttcttgc gagcctcgcg ctgcaacttc
     7021 tcctcctcat cgtggttcag cgcgccgccg atacgcttac tggtctccga gaatggtttc
     7081 gtaatcgcgg cggtcaggct ccgggcgggc cgcaatacct cggcggcacc gagctgaacg
     7141 gtgtgcagcg gcccgcagcc gctcttgaca tccgtgcagt caccctcctt aacgtagacc
     7201 gtaaacagca cgagacacgc tatacagagg ataaagaggg ccgcgagacc gctggcggcg
     7261 ctggatttac tgcgtcccac gatgaccggc tagttggagg tcagggcgct gcggtagtag
     7321 tcgatctctt ccagcgaccg gccgcttccg agggcgacgc acatcagcgc atcctccgcg
     7381 acgtggaccg gaatcccggt ctcacggcgc agccgctcat ccatgtgccg caacagggcg
     7441 ccgcctccgg cgagcaccat cccccggtcc atgatgtccg aggcgagctc cggcggcgtg
     7501 cggtccaacg tgtcccggac agcggcgacg atcgcgtcga cagggacgct gatcgcctct
     7561 cgcacctcct cgctcgtgat cacgatggtc ttcggaagac cagtcacgag gtcccggccg
     7621 cggatctccg ccgactcctc ttcctccagc cggaacgcgc tcccaagctc tatctttagc
     7681 tgctccgccg tctgcgtgcc tatagcgagc ttgtactcct tctgaatata tgtcgtgatc
     7741 gcatcgtcca cgtcatcccc ggctatgcga atggacgact tcgtgactat cccgccgagc
     7801 gagataacgg cgacctcact cgtgccgccg ccgacgtcca cgatcatgga cccctgcgcc
     7861 tcgttgaccg gcagccccgc cccgatagcc gccgccagag gctcctctat cgtgtaagcc
     7921 tgccgcgccc ccgccgactc ggtcgcctcc ttgaccgccc tgagctccac gcccgtaacc
     7981 cctgaaggca cgcacaccac gacccgcgga cccaccaacg agcggaagat gcggcgctgc
     8041 ggctgcacct tgcggataaa gtacgagagc atcttctcag tgacctcaaa gtccgcgatc
     8101 acaccgtcct tcaacggacg catcgctata atgttgcccg gcgtgcgacc gagcatgcgt
     8161 ttggccgccg atcccacggc gaccaccttg tcggtcttgt tgtctatcgc taccaccgaa
     8221 ggctcggaga gcacgattcc ctggcctttg acgaacacca gcgtgttcgc cgtgccgagg
     8281 tctatcgcga cgtcccgccc gaacagtcct ccaaacatac cgcgtcccat ctgttcaaat
     8341 ttcaggctcc gaaccgtaat gatacagccc tagagcatcc catgttcctt catgctgaag
     8401 tacccatcgc ccacgattac gtggtccagc acctctatgc cgagtatctc ggccgcgtcg
     8461 acgagcctgc ccgttacctg gcgatcctcg cggctcggtt ctaccctgcc gctcggatgg
     8521 ttatgcgcca gcacgacgct agccgcgctc gcccggacag ccggtttaaa gacctcgcgg
     8581 ggatggacga gagagttcga tagcgtgccg atggagaccg tcgagatctc cagtacctca
     8641 ttcttcgtat tgagcagcac ggcgacgaaa ttctctcgat cacgattcgc gatccgcccc
     8701 ctcaagagcc cgtcaacgtc cgctggcgat gatatcacgg gccgaccggg gttgcgaact
     8761 ctgccgagcc gttttccaaa ctctaccgcc gccagtatta tgcacgcttt tgcctctccg
     8821 acaccgtgga tcttgacgag gtcatggacg gagacgttgt ggagcccgaa gaggtccccg
     8881 ctatcggaga tcacctcgct ggcgagctcg accgccgtct tctcgcggct accaatgccg
     8941 aacatgatgc ccagaagctc cgcgctggag agcgcctccg gcccctcgga gagcaggcgc
     9001 tcacgtggac gcagttcggg cggcagttgt tttatcgtgt agcgtcggcg ctccattggg
     9061 acattctaat agagacgcac cccgaaacca gcgagcatcc ggcctgtcag ggccagcggg
     9121 agcccgacga cgtttgtgta atcgccgcca atccactcta cgaaacgagc agcccggccc
     9181 tgaatcgcgt agcctcccgc ctttccgata ccttccccgg acagcgcata ggcttcgcgt
     9241 tcctcccgcg gtatctcccg catcctgacg ctcgttatcg agtgacggat aatgatctgc
     9301 tcaccacggg tcaccgccac gcccgagtaa acttcatgga cgcggccgtg aagcaaggac
     9361 agcatccggc gaacttcctc gggatcgccg gcctgaccaa agatccgctc gtcaatgggg
     9421 agatcggtcc ggtacacgac cgtgtcagcg gccaagacga ctctacccgg ttcgatcttg
     9481 gcgacggcca gggccttgcc gcgggcattc gcgagaaccg tcgctaccgg accttcgagc
     9541 gtaacctctg ggaagccgct cttgcgcgtc tcaaaatcgt agcccgcctg gtggagcaga
     9601 tccacgcggc gggccgattc cgatgccagt atgaatcgcg agtctggcag cttactcgaa
     9661 gaagagcttg agctcgcggg cagcactctc aggggagtcg gagccgtgga cgaggttgtc
     9721 gggcatgctg agggcgagat ctccccggat cgtaccgggc gcggcctccg ccgggttggt
     9781 ggaacccatg aggttgcgca tgaccttgat cgcgccctcc ccctcgacgc gcatggcgac
     9841 aaccggcgtt gaggttatga actcgaccag ctctccgaag aaaggtttct cgcggtgctc
     9901 ggcgtagtgc tgctcggcca gctctcggct cacgtccatc agcttgaggc cacggatatc
     9961 caggttcttc gcctcgatgc ggcggataat ctcaccgata agacgccggc gcacgccgtc
    10021 tccttttacc agtatcaggg tctgttccat tgttgtctcc ttctagaggt tgattcgttt
    10081 gccgctgcgc tggttggtgc cgcagtacgg gcacgtctcc cattccatct tgagcggttt
    10141 gtcgcactgg atgcacgact tcttgagctc ccagttgcag ttcgggcaga ccaggtaatc
    10201 cttctccacg atattgcggc agttcggaca gagtaagacc cggttgcgaa gctcgccttc
    10261 gagcacggca agctcaagct cccgctcgcg ggagtccagg aggtactcgg gaggacgcac
    10321 tatcaggtag ataagcgtgc cgagaaacgg gaatatcacc gcgactatgc cccagaagat
    10381 cttcatggcg ccgcgccgtg aggcgtcggt gtaggtccag tagatcagcg ctatccacag
    10441 gcacgctacc agcaatagga gtaactgccc cgcgatacgc aggaacgggc tcgtcaggaa
    10501 gttctctatt atcttgaacg ggtcggtatc ctgctggagt agcgggatct gcagcgcggc
    10561 gttcaaagga tgaacctgat catggcgaag tagccgacgg tgaaagatac agcggccagg
    10621 atgagcagca gcacggctaa tttccaggtc ccgctaacct tcacgaagcg cccccagaac
    10681 cgccgctcca gtgtacaaag atccagtcac taacaccact ccgtctactc tcctcatctc
    10741 ctcgaccgca acttcgagag catcaccggc gtatgttacc accagcgacc gtggatctcc
    10801 ggcgcgtacc cggcgaagct catcagccac tgcctccggc tccgcagccc ggtcgcttag
    10861 aggacgagta attactatga gatccacctc gcccgccaga gcggcgagca ttctcgcagc
    10921 gtctttatcc cgcaacacac cgaagatcaa acccagcggc cggctgccat atagccgccg
    10981 caccccgccg agcgcggcct ctatcccggc ggcgttgtgg cctccatcca ccacgaccgg
    11041 cacgcctctg acctcatgga cctcgaaacg ggccggcaac acccccgcga cgctccgcgc
    11101 caccctctcg cgttcctccc agcccaggtc tcgaccggtg atgttttccg cgaccagcac
    11161 acctagccgc acgttgcctt cggcatacgg cggaagtcca gcctctgctc cccgatccgg
    11221 cttcctgcgc tcctcctctg gaggcagctc tacggtccgc gccttcacgg aggccgctac
    11281 accgcgagcg acgttcagga cggcgggatc gccggtgccg aggaagagag tcgcgccggg
    11341 ccgcaggctc gccagcttct ccctggagat ctcctcgacc gtctcaccca ggtactcggt
    11401 gtgatcgagg cttacattcg tcagcactac agcctctgcc ggcgccgccg aggtcgcatc
    11461 gtggcgagcg ccgaggccag cctccagtac ggcgaagtcg agacccgcat catgaaagag
    11521 cttgatggct ccggccgtca gcagctcgaa ctgcgacgcc ggaacgccgt tcgcgtcggc
    11581 gagcgcgata gtctcaccca tcgccccggc gaagtcttct tcggtgatga atcgaccccg
    11641 gatcatcacc cgctcggtgt atgagagtac gtgcggcgag aggtacgcgc cggaggtaaa
    11701 tcccaacgtt tcgagcgcgg aagctatcgc cacagcggtg gtccccttgc cgttagtacc
    11761 cacgacctgc actatccgca gcccacgctc cggtttgccg agaaggttca agagtctctc
    11821 tatgcgctcg aaaccaagag ttacgtggcg gcggcggtcg agttcttcac agacctcgtg
    11881 gaacaagcgg ggatgctcca acggtttaca ggtagtcttg gagccgctgg ttcagcgtgt
    11941 cgagcatgcg ggtgttcacg tcgagcttct cgcgctcgcc agcgacgacg tgtccggggg
    12001 cgcgctccac gaacttctca ttcgagagct tgccctgacc gcgctttacc tctccttcga
    12061 cccgcgagat ctctttgcga aggcgctcga tctcgccgcg ccgcaactcc tccgtcaggg
    12121 ccacctctac caccacgtcg ccagcaggga gcgccgcgcg gaacgctccg tccatgtcct
    12181 gcacgggcga cacaccggcc agcgcctgga agacatcggc ttccacaccg tccggcaacc
    12241 ggccgtcgag ctctccttcg accctagact cggcccggaa ggacctcacg gcagagacgg
    12301 cgcgcctggt gcgttcgagc agccggtccg cttccgcatc ttcgagcgcc gcatcgaagg
    12361 cggggaactc ctggcgtatc agcattccct tgccaccgag cacgcggtac atctcctcgg
    12421 tcacgaacgg catgacggga tgcagcagct tgagtatccc ggtgaagacc tcgcggagca
    12481 cgccaggcgt cgcgggtgaa ggcacggcct tcgctatctc tatgtaccag tcggcgaact
    12541 ggtgccaggc gaagtcgtag atccggcgca tcgcctccga aaactcgcac tcttccagaa
    12601 gcgcgtcgta ctcacgagcc gcgcgattga actccgagag gatccaacgg tccgaaacgg
    12661 acagctctcc gctctcttcc gcctcggggt aactgagcac gaaccgcgtc gcgttccaca
    12721 gcttggtaac gaacgcgcgg cccatggagg ctcgctcgta ggagtaggcg aaatcctggg
    12781 tggaggactg gtagagcagc ccgaaccgta cggcgtcggt gccgtactcg tcgaacaggt
    12841 cgagcgggtt gatggtgttc cccttcgact tgctcatctt ggtgccatcc tctgcgagga
    12901 cgatggagtg gacgttgatc tttcggaacg gcacctcgcc cctgaagcgc agcccgctca
    12961 tgatcatgcg cgcgacccac aggtacatga tctcccgcgc cgtcgccaga agactcgtcg
    13021 gataaaagta ctccaggtcc tcgctctcct cgggccatcc gagcgtggcg aacggccaca
    13081 gaccactgct gaaccacgta tccaggacgt ccgggtcctg ctcgaagcca gtgcccggcg
    13141 actctttaga tgcgacgacc tcgccgtccg ggccatacca gaccgggata cgatgccccc
    13201 accagagctg gcgcgacacg ttccaatcct ggaggttctc aagccaccgg atcgactccc
    13261 gccgccacga gtcggggtag acgttgatct tacgctcctg gagagcctcg atggcgggtt
    13321 ccgccagctc tcgcatcgcg acccaccact gctccgagag ccagggctcg atgaccgtcc
    13381 cgcacctgtc gcagtgcccg acccggtgcg gatagtcctt gacctctccg agcagcccct
    13441 ccgctccgag tccttccacc acggccttcc gggcgtcctc ccggctcatc ccctcgaact
    13501 tcgcgccgtt ggcgttgatc gtaccgtccg ggttcaggac gttgacgggc gcgagtccca
    13561 gccgctgacc gatatcgaag tcgttcgggt cgtgggcggg tgtgaccttg agcacgccgg
    13621 tgccgaacga cgggtccaca tagccgtcgg cgaagacctc gatctcccgg tccacgaacg
    13681 gcacccacac tcggcgcccg accagctccg tatatctttc gtcgtccggg tttacgacga
    13741 gcgccacgtc gcccagcatg gtctccgggc gggtcgagaa gacctgtgcg tagtccctgc
    13801 cgtccggtcc gggacccttg ccgtccacga acgggtaacg aaggccgtag agcttgccgt
    13861 tcacctcctc gtagttgacc tcaaggtccg agatggcgga gcggtcgtgg ggacaccagt
    13921 tggtgatgcg gttcccctta tatatgagac cctcgttgaa aagcttcacg aaagcagtca
    13981 gtactgcgtc tatgtactcc tcgtccatcg tgtagcggag cctgcgccag tcggccgagg
    14041 ctccgagacg ccggatctgg ccgaggatct gggagcgcga ctctgcggcg aactctcggc
    14101 aacgttcaac gaactcttcg cgcccgatgt cgaatcgggt cttcccctct tcgcgcatga
    14161 gcttgcgctc gacgacgttg tgcagcgcta tggaggcgtg gtccaccccc ggcaaccaca
    14221 gcgcctcgta gcccttcatt ctggcatgac ggatgaacgt atcctggatc gagaaggtaa
    14281 gcgcgtgccc catgtggagt gcccccgtca cgttcggcgg aggcatcacg atgcagaagg
    14341 cttccttctc cgggttcggg tccgcgtcga aggcgccgga gcgctcccac tcagcgtaga
    14401 ctctctcctc gacctctctg gggtcgtatg tctttggtat ctcggcctgg ctcaagaggc
    14461 ggtgcgggga gtctcttcgt cgtatgagta gtccgacccg gagcccgtta cgagcgaggg
    14521 ctgtacgcct tccttgaccg tgtccgcgtc cacgacgcac ttggatacgt ccgaccggct
    14581 cggaagctcg tacatagtcg ggagcagagc cgtctccagg atgctccgca ggccgcgcgc
    14641 tccggtgccg cgctccagcg cgagctctgc caccgccttg agggcgtcct cggtgaagtt
    14701 caggtccacc ttgtcgtaac ggaagaactg ccggtactgg cgcacgagag cgttcttcgg
    14761 ctcggtgagg atgcggataa gatccttctc ctccaggcta cggagcgtcg agatgacggg
    14821 cagacgcccc acgaactcag ggatgagccc gtacttgaga agatcctcgg gctgcacgtg
    14881 tttgaactcg gcgcccggct cctcctcgac cctcgtggtc gtgtcgctgc cgaagccgat
    14941 gctcttcttg cctatgcgct gccggatgac ctcctccagc ccgccgaagg caccgccgca
    15001 gatgaacagg atgttcttcg tgtctatctg caggaagtcc tggtgaggat gcttgcgtcc
    15061 accctgcggc ggcacgctcg ctacggtgcc ctccaggatc ttcaggagcg cctgctgcac
    15121 gccctcgccc gagacgtcgc gggtgatcga ggggttatcg gacttgcggg cgatcttgtc
    15181 gatctcgtct atgtaaatga tgcccgtctc ggccttcttc acgtcgaagt cggcggcctg
    15241 gatgagcttg aggagtatat tctccacgtc ctcgcccacg tagccggcct cggtgagcgc
    15301 ggtggcgtcg gcgatggcga agggaacgtt caggatgcgg gcgagcgtct gcgcgagcag
    15361 cgtcttcccc gaacccgtgg gaccgagcag caggatgttc gacttctgca gctcggtgcc
    15421 gtcggaagaa tcgggcccca tcgagatcct cttgtagtgg ttgtagactg cgacggcgag
    15481 caccttcttc gcgtccgcct ggcctataac gtactcgttg aggatgcggt tgatctcccg
    15541 gggcttcggg aggtcgtcgt ccttcaggac ctccgggccg gagaactcct cgtcgatgat
    15601 ctcattgcag agctcgatgc actcgtcgca gatgtagacg ccgggtcctg ctatgagctt
    15661 cctgacctgg cgctggctct tcccgcagaa agagcactgc aactggtctg acgggtccct
    15721 catgagttac ggcctcccga gcctcgctcc gcttgcacgt cccctcccgc cggcatacta
    15781 atgctgccgg acgatgttgt cgatgacacc gtactcgacg ccctcctcgg cccccattat
    15841 gtagtcccgg tcggtgtcgc gctcgatctt gtcatactcc tgtccggtgt tctcggccag
    15901 gatctggttc agtcgctgct tggtgaggat gagctccttg gcgtggatct ccacgtcgga
    15961 agcctgtccc gccagtccac caacgctcgg ctggtgcagc aatatgcggg tgttcggcag
    16021 cgcgttcctc ttacctttcg caccggcggc gagcaggaag gcgcccatcg aggcggccat
    16081 gcccagcgcc gtcgtcgaga tgtccggctt cacgaactgc atcgtgtcgt agatcgccag
    16141 ccccgccgta acactgccgc ccggcgagtt tatgtacagg ttgatgtcct gctccgggtc
    16201 ctcgctttcg aggtgcagta gctgcgccat gatggcgttc gctacctggt catccaccgg
    16261 cgtcccaagg aagatgatgc ggtccttgag gagccgcgag tatatgtcca tcgcccgctc
    16321 accgcgcggg ctctgctcta taacatacgg gattacaccc tgcggatcgt aatagctcac
    16381 tgctgctctc cttcgctagt ctctgtctcg ccgcccggct cagcgacggc cactgcctcg
    16441 tcggaaccct cttctgacgg ttcttcttcc gccggctcct cttcctccgg catctccacg
    16501 gcgacggcgt tctcgaccag gtagtcgagc gccttctgcc gggccatctc ttcctgcagg
    16561 agagcgaagg tgccgttcgc gcgcatcatc gctgcgatct gctctggaga ttgctcgctg
    16621 ccttcggcga tgtgcccgat ctcgtgcatt accgcttcgt catcggcctc gataccttcc
    16681 ttcgcggcga ccgcgtcgag caccagttcc ttcttcacgg tgtcttccgc gtcgggccgg
    16741 acgcgctcct ccatgtcgtg gtggctggcg ccggcgagct ggtagtactg ctccggctcc
    16801 atcccctgag cccggatgct ccgctcgaag ctctcgacca tctcgtgagc cttctcgtgg
    16861 accatgacct ccgggatctc gacctcggcc cgctcggcca ccgcgtccag cactcgtccg
    16921 cggaactcgc cctcgacccg ctgctctatc gccgcctcta gctgctccct caccgcagag
    16981 cgcaactcgt ccagcgtctc gaactcgctc gcctccttgg cgaagtcgtc gtccagcggc
    17041 ggcaggtcgc gctcctttat ctccttgagg tggacgtgga agaggaccgc ctgcccgcgc
    17101 agcgactcct cggcatagtc catcggaaac gtaactccga actgctttcg ctccccggcg
    17161 ttcatcccga cgaggttctt ctcgaagtcc tccaggagct cgccgcgacc gatctccagc
    17221 atgtagtcct cggcgtcgcc gccctccagc ggttcgccgg tcgccatccg ctcgccccgg
    17281 aagtcaatga tcgtgaaatc gccctcctgc gcggcacggt cctcgaccgc cgcgagggtg
    17341 gcgaactgtc cgcgcatctc ctctagctgc gcctcgacct gctcgtccgt tacctcgacc
    17401 tcgcgcttcg ggacctcaag acccgtgtac tcgccgagct tcgcctccgg gcgaacttcc
    17461 acggtcgccg agaacttgaa gccctccttc tcatccatcg ggtcgtcgaa ctcgatctcg
    17521 gggcggtcta tcgggcgcag atcggtctcg accacggcgc tcgaatacca gttcgggagc
    17581 tgctcctgga gcgcctccat gaagatgtag tcgcggccga cgtggttctc tataacccgc
    17641 cgcggagcct taccgggacg gaaccccggc acccgcacct ggcggccaag ctccttgacc
    17701 ttcgcctcga ctcccttgcg gacggcctcc cccgagacct ctacttccag ccggacctta
    17761 ttgtcttcca gcttcgttac gttcgccgtc atacgctcca actcttctcg actaccatcc
    17821 cggccacagc agagctacgg cagagtgcga gaggcgggac ttgaacccgc acgccgctag
    17881 ggcactagat cctaaatcta gcgtgtctac cattccacca ctctcgctcg cctcatagat
    17941 cagatagaaa tccacggccg gattataaca gccacccctc ccccgcccgg ctctacagcg
    18001 ccctttacgc taacggcgca caggctttgc tccgcccgac ttcatggccg cgcaacgccg
    18061 ttcagggcgc ctggtagcga aactcccgca ggagtgcctg gttcgcctgc gtgtcgggcc
    18121 tgagcacctg ctcgccgtcc ggcagggtct cgggagtccc cttgagctgg gtcgcggtca
    18181 tcctcgcctc gcgtccgtgc tgcagcaaga cacgtcccag agtaatggcc tcctgtacgc
    18241 ccatgtctgt ctggacgtga tcctcaatag tggcggcgac ggacggcagc tttcgcacac
    18301 cgttccagcg gagtgccttg cttctcaggg cggcgacgag ttgctgctgg cgctctatgc
    18361 gatcgaggtc cccacccgga gtgccacggt accgggcgta gagcagggcg cggcgaccgt
    18421 tgagatgttg cacgccttcc ttgatatgaa cattgggtgg aaagccgccc ttggaatcga
    18481 cggtaacgcc cccgatatca tccacgacgg cctcgaaacc ctcgaagtcc actatagcgt
    18541 agcggtctat gcggatgtcg gcgtaattct ccagcgcgga taccgtctga gagaccccac
    18601 catacgagta agccgcgttg accttgctct cgacccccgg cgacacctcc accatcaggt
    18661 cccgcggcac cgaaagcagc ttgatcatgc cgctctgcgg cgatatccgc accagcatta
    18721 tggtgtccga gcgcctcgcc gtgctaccgc tgccatcgtt cggcttatcg tcgacaccga
    18781 gaaccagtat gttgatgggg tctcccccga gcaggctgcc gccgttcttg tcgaatgagg
    18841 catgtgtcgc cttcaagttg acgagagggt tgagatcgct ggcccggtca acccccagcg
    18901 accgggccag aggagcgccc cccacctgcg cgccagctcc aatcaagacg gccaagaccg
    18961 cggccacgca agcagacatc aacaaccacc ggctccggga cgtgccggcg ttgcgcgtat
    19021 aatcctggaa ctccctcacc cggcgaagtc taatccccgc ggtgcgctac cgcaacgcgg
    19081 cccgttaatc caggggatca gacacagccc ggcaccttcc cggatcgcgc agtttctagc
    19141 agcgccgtga tctccccggt cgatggcgac tctcgaagag gatcttcgcg cgacttaccc
    19201 gtcaccatct agaatgagga gtccggcatg cagcgtctcg cccatggctg aaccgggagc
    19261 cggcaacacg gactcctgta gcgcgccttc aggaagccgg acatccgcgc cgggcgagcg
    19321 cttctgttga ctatccctga gcggctggct cgcgggttcc gttaccggcg ccgacttcct
    19381 cttccaccgt ccggcctcgc ggctcaacgt ccggctccgc atgttcaccg cgagccgtca
    19441 gaacgtcgcg gataaacaga tcatagacaa acgctcccac cacgccgccg atcaacggtc
    19501 cgacgattgg cacccaaaaa tagtagttca cgccgggcag cgccacgtcg ccccatccgg
    19561 cgagccacgc aaacacccgg ggaccgaggt cacgagcggg gttgatagcg tagcctgcat
    19621 tcgcgccgaa agacataccg atcgccgcga ccgcgagccc caccaggagc ggggccatgt
    19681 tggacttcgg cggctggttg aggacgtcca cgagcgcgaa gacgatgagc accaggaaag
    19741 cggtgccgat gatttggtct atcaagggtc ctatgttgct gccgttaaaa taaggcgcgg
    19801 ggaatgtcgc gaagatgctg aaagtcgcgg tcgagtccgc cgcgccccgc gttatgttct
    19861 tcgcgttctc gaagctcgct atggcgttat ggtagttgag atagaccagc gcggctcccg
    19921 cgaaggcgcc gaccatctgg gccgccatat atccgggaac cttgctccac gggaagcccc
    19981 gtttcaccgc gaaggccagc gtcacagccg gattgatgtg cgcgccgcta ataccccccg
    20041 ccacgtagac cgcaaaggtg acggccaagg cccatccaaa ggctataagc agccagtcgc
    20101 cgctagccgc gaagatctcc ttgccacgcc ccgactggtt caacgcggcc acggtcatgg
    20161 ctaccacgcc gttgccgaac gcgatcagga cgaaagtacc caggaactcc gcggatagct
    20221 ccccccatat cgtagaccgc catcctcccc gggccggtgt cgataccgct gacatgattg
    20281 tttctcctct ctatacctga cgccgctcga agcaagcccg gctcaagctg ggatgctcac
    20341 ccaaccgaat acctggcccg ccggacggcg cgtcgcggtc cggttgccgg atgcgtcagc
    20401 ttcacgatga agcagatagc cgctgtagcc gggcgaggtc gaagttcatc acgtacgcgc
    20461 agttctcaca gataagctgc gcgcgctcca ggcgatgacc tccgtaacct tcctgcattc
    20521 cggcaccgag aaccgtagcc tcttccatac ggaactcttc agccccgcat acgggacagg
    20581 ccaggtttcc accccttgac tgaatagacc ggcgcatctc ttccaagctc atatgtagtc
    20641 tggtcgccat gtcacgcacc tccttttctc gttcggtaac gcggtacaga gcccatcgtt
    20701 catacaccta gcggtctaca gacacccacc tccagacacc ttgccaacca ggtcgtaatc
    20761 ctgttgggga tcgagagacc acgcttccac tccacaccgg aacaggttct cgcaggcgat
    20821 ggggtaaagc gccggacgta ctcccggaat actcacgtag cggacgatag agagcgttga
    20881 ctatcagccc ccgaaaggat aagaggtgtc ttggcacttt cccactcttt cccgttctta
    20941 tcacatagtt tacatccgct tctagatcac cttttaagat cctattaagg ctctttaaac
    21001 ttctcattaa actgttcgtc gctcttgccc tcagcgagcc gcaggaggca actcctccgc
    21061 aaaacagcct ccctggcttg cggagtgaga ccctccacat gcaccagaga ctgacccata
    21121 tgcaacgatg tcatttcacc gaatcggcag cttcgaccgc accatttgcc gccggtcagt
    21181 acctctctcc ccggcaaaat catgaaaggc atgggcgaaa agatcagagg gatcaagaga
    21241 agaacctgga aacgatcaac cagagacgga tttcgacatc gtacgcatcc cgtccgtacc
    21301 attcgggcag ggacatccat cgatctcgtt caggacagga gggtgaccga cggggctcga
    21361 acccgcgacc ttcggagcca caatccgatg ctctgccaac tgagctacgg ccaccatgta
    21421 cgcggatgat tctaccaaca atcgggcgcg cccggcagga ctcgaacctg caacctgcgg
    21481 attagaagtc cgctgctcta tccagttgag ctacgggcgc acgctacgcg cggcggtaga
    21541 gagcgggtga ggggaatcga acccccataa ccagcttgga aggctg
//
LOCUS       DASSMB010000069         3665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_776023, whole genome shotgun sequence.
ACCESSION   DASSMB010000069 DASSMB010000000
VERSION     DASSMB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776023"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VFJ72_04725"
     CDS             complement(<1..525)
                     /locus_tag="VFJ72_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7478803.1"
                     /translation="MIEIEGLSFTYRTGEEDAVPALRDLDLRIDRGQFVAVIGHNGSG
                     KSTLVKLLTAIMYPTEGGIRIDGVPVTEENQWEIRRRVSVVFQDPDDQLVMNRLEDDV
                     AFGPENLGLPREEIGERVESSLRALGLEDIRGRLIEDLSSGQKQRVAIAGALAMRPHF
                     LILDEPTSLLPVPVA"
     gene            complement(571..1170)
                     /locus_tag="VFJ72_04730"
     CDS             complement(571..1170)
                     /locus_tag="VFJ72_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7478804.1"
                     /translation="MATGTGSERRGSGFGLGVRQIVIAGVLGAIAVFLGATRLGFIPV
                     PNLAGSATIMHVPAILGSALEGPVVGVIVGGIFGIYSWLYAEIPLFKDPIVAIVPRLL
                     IGVVAWLVFVGLKRWSVDLASAAAGVLGSFTNTVGVLGLGVLFGYLPLAAVVPILPQA
                     IAEAVLAAVVTVVVVRGVMLVRSGRTTATETRSDEERRY"
     gene            complement(1273..2055)
                     /locus_tag="VFJ72_04735"
     CDS             complement(1273..2055)
                     /locus_tag="VFJ72_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7478805.1"
                     /translation="MFDYARMFDLTGKTALVVGAGSGIGEASAHGLAAFGAEVLAADV
                     NAEAAEATAAEVRKSGGKSVAVGMDMLNSESVEAIAQEIGTPDVLVSTPSVNVRKPVL
                     EISDEEFDRIVNLNLKGTFRLVRQFGRGMAERGSGSIIAFASIRAQTVEPGQGIYAAT
                     KAGTVQMLRALAAELGPRGVRVNAIAPGVVETPLTAQIKNNPEWYDAYAKGGILGRWA
                     QPHEMVGMVVYLASEASSYVTGGFMLVDGGWTAADGRFTPPL"
     gene            complement(2048..3322)
                     /locus_tag="VFJ72_04740"
     CDS             complement(2048..3322)
                     /locus_tag="VFJ72_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7478806.1"
                     /translation="MPELPGETAADPEFEELLDAVLRRIDREELVRLTQDLVRIPSVY
                     HPDDPDGNETRAARFVADYLEHAGFDVRTENVAPGRPNVWTVWEGDRPGRTLLFEGHT
                     DVVTEGDAEDWTYPPFEAERFDGRIYGRGACDTKGNLAAAMVAVRAIKDSGIPLPGRL
                     VLCHPVDEEGMMTGIKAFIERGYADGVDGAVICEPEENQLCVKQKGALRVEVTVRGRM
                     AHGAMPLSGVNPVTRAARFVVAVEELEKEEIERHGEDPFLGYPSLTPTILMGPDCGEP
                     QINVIPASAYVALDIRTTPSQSHEGLVERLREILARLKFADPDFGATLEVIEERPPTE
                     TPMDDPLVRAMAAAYTRLTGEEPRYNGVPGATDGTFLHARAGIPIVTTGAGNREIPHH
                     KDEWVGEEELFTTCKLYAATAMYYCYGKDADV"
     gene            complement(3326..>3665)
                     /locus_tag="VFJ72_04745"
     CDS             complement(3326..>3665)
                     /locus_tag="VFJ72_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7478807.1"
                     /translation="QMLALTIAALGIYSALATFWSLPTAFLSGTAAAAGIALVNSVGN
                     LGGFVGPYVVGFLSDRTGSTYGGMLFLAVLILLAGLVAVLAVRHERSLEEVEEVVTPT
                     IGASGTPASE"
BASE COUNT          577 a         1355 c         1132 g          601 t
ORIGIN      
        1 ggcgaccggg accgggagca gcgaggtcgg ctcgtcgagg atgaggaagt gaggacgcat
       61 ggctagcgct ccagctatgg cgacgcgctg cttctggccg gaggagaggt cttcgataag
      121 ccgcccacgg atgtcttcca gtcctagcgc ccgcaacgag gactcgaccc gctcccctat
      181 ctcctcgcgc ggcagcccaa ggttctcggg gccgaaggcc acgtcgtcct ccagccggtt
      241 catgacgagc tggtcgtcgg ggtcctggaa cacgaccgag acccggcgcc ggatctccca
      301 ctggttctcc tccgtcaccg ggacgccgtc tatccggata ccaccctccg tcgggtacat
      361 gatggcggtc aggagcttga cgagcgtgct cttccccgac ccgttatggc cgatgaccgc
      421 cacgaactgc ccccgatcta tccgcaggtc gaggtcgcgc agcgcgggga cggcatcctc
      481 ctcacccgtc cggtaagtga atgagagccc ttctatctcg atcatcgcgc tcgcatatgc
      541 gctcgcgctt gcttccgcgt caccccgcgg ctagtaccgg cgctcttcgt cggaccgcgt
      601 ctccgtggcc gtggtacgtc cgctgcggac cagcattacg ccccgcacca ccacgaccgt
      661 gacgacggcg gccaggaccg cctcggctat ggcctgcggg agtatcggca cgaccgccgc
      721 cagcggcagg tatccgaaca gcacacccag gccgagcacc ccgaccgtgt tggtgaacga
      781 gcccaggacc ccggccgccg cggacgcgag gtccacgctc cacctcttga gccccacgaa
      841 cacgagccac gccaccacgc cgatcaggag ccgcggcact atagccacta tcgggtcctt
      901 gaagagcggt atctcagcgt acagccagga gtagatgccg aagatgccgc ccacgatgac
      961 cccgacaacc ggaccttcga gggcgctccc caggatcgcg ggtacgtgca tgatcgtcgc
     1021 gctgcccgcc aggttcggga ccgggatgaa tcccagccgc gtcgccccta gaaacaccgc
     1081 gatggccccc agaacccccg ctatgactat ctgccggacg ccgagcccga accccgagcc
     1141 gcgcctctca ctacccgtac ccgtcgccat gaaccgcctc cttttccccg attgtattac
     1201 cggcaggaca gcaaagttgc tcccgctcgg cctaccgaag tatccgtatc tatatgcaaa
     1261 ctgtcaatac atctacagcg gcggcgtgaa gcgtccgtcg gcggcggtcc agccgccgtc
     1321 cacgagcatg aagccgccgg tgacgtagga gctcgcctcg gaggccaggt agacgaccat
     1381 accgaccatc tcgtgcggct gcgcccagcg gcccaggatg ccccccttcg cgtaggcgtc
     1441 gtaccactcg gggttgttct tgatctgggc cgtcagcggc gtctcgacca caccgggcgc
     1501 gatagcgttc acgcggacgc cgcgaggtcc gagctcggcg gcgagtgccc ggagcatctg
     1561 gacggtgccg gccttcgtcg cggcgtagat gccctggccc ggctccaccg tctgggcgcg
     1621 gatgctggcg aaggcgatga tgctccccga gccgcgctcg gccataccgc gcccgaactg
     1681 gcggacgagg cggaaagtgc ccttcaggtt caggttcact atccggtcga actcttcgtc
     1741 cgagatctcc agcaccggct tcctgacgtt gacgctcggc gtgctcacca gcacgtcggg
     1801 cgtgccgatc tcctgcgcta tcgcctcgac gctctccgag ttgagcatgt ccatcccgac
     1861 ggccacgctc ttgccgccgc tctttcgcac ctccgccgcc gtcgcctccg ccgcctcggc
     1921 gttcacgtcc gccgcgagga cctcggcccc gaacgccgcc agcccgtggg cgctcgcctc
     1981 cccgataccg ctccccgccc cgaccacgag cgcggtcttg ccggtcaggt cgaacatcct
     2041 ggcgtagtca aacatcggcg tcctttccgt agcagtagta catcgccgtc gcggcgtaga
     2101 gcttgcaggt ggtgaacagc tcctcctcgc ccacccactc gtccttatga tgcggtatct
     2161 cgcggtttcc cgcgcccgtg gtgacgatgg ggatgccggc ccgagcgtgc aggaaagtgc
     2221 cgtccgtcgc gccgggcacg ccgttgtagc gcggctcctc gccggtgagc cgggtgtagg
     2281 ccgccgccat cgcccggacc agcggatcat ccatcggggt ctcggtcgga gggcgctcct
     2341 cgatcacctc cagcgtcgcg ccgaagtccg ggtccgcgaa cttcaggcgg gcgaggatct
     2401 cccgcaggcg ttcgaccagc ccctcgtgcg actggctcgg ggttgtgcgg atgtccaggg
     2461 cgacgtaggc gctggcgggg atcacgttaa tctggggctc gccgcaatcc ggtcccatca
     2521 ggatcgtcgg cgtcaggctc gggtatccca ggaacgggtc ctcaccgtga cgctcgatct
     2581 cctctttctc caactcctcg accgccacta cgaaccgggc cgcccgcgtc accgggttga
     2641 cgccgctcag gggcatcgcc ccatgcgcca tccggccccg caccgttacc tccacccgca
     2701 gagcgccctt ctgcttcacg cagagctggt tctcctccgg ctcgcagatg accgcgccgt
     2761 ccaccccatc ggcgtatccg cgctcgatga acgccttgat gccggtcatc atgccctctt
     2821 catccaccgg atggcagagg acgagtctgc cgggtaacgg gatgcccgag tccttgatgg
     2881 cgcgcaccgc caccatagcc gctgcaaggt tccccttcgt gtcgcaagcc ccgcgcccgt
     2941 agatgcggcc atcgaaccgc tccgcctcga agggcgggta cgtccagtcc tccgcatctc
     3001 cctccgtcac cacgtccgtg tgtccctcga agagcagggt cctgccgggc cggtcgccct
     3061 cccacaccgt ccagacgttc gggcggcccg gcgcaacgtt ctccgtccgc acgtcgaagc
     3121 cggcgtgttc gaggtagtcg gcgacgaacc gggcagcgcg ggtctcgttg ccgtcgggat
     3181 catcgggatg gtagacgctc gggatgcgga ccagatcctg cgtcaggcgc acaagctctt
     3241 cgcggtcgat ccggcgcaag acggcgtcga ggagttcctc gaactcggga tctgcggctg
     3301 tctcccccgg cagctccggc aacggctact cgctcgccgg tgtgccgctc gccccgatgg
     3361 taggcgtcac gacctcctcg acctcctcca ggctgcgctc gtgccggacg gcaaggacgg
     3421 cgacgaggcc ggccagcagg atcaggaccg ccaggaagag catcccaccg taagtagatc
     3481 cagtcctgtc gctcaggaaa ccgacgacgt agggaccgac gaagccgccg aggttcccga
     3541 ccgagttgac cagcgcgatc cccgcggccg ccgccgtgcc gctcaggaac gccgtcggca
     3601 gcgaccagaa cgtcgccagc gcgctgtaaa tgccgagcgc cgcgatggtc agggccagca
     3661 tctgg
//
LOCUS       DASSMB010000070         3421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1537891, whole genome shotgun sequence.
ACCESSION   DASSMB010000070 DASSMB010000000
VERSION     DASSMB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537891"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VFJ72_04750"
     CDS             <1..1308
                     /locus_tag="VFJ72_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HET7478808.1"
                     /translation="APDSSYGTPDDLKGLIQAAHQKGLQVFLDVVYNHFGPEGNYLGV
                     YAPDFFTGRHETPWGAAINVDGEGSEHVREFFIHNALYWLEEYGFDGLRLDAVHAIID
                     DSPKHLLVELAERVHEGPSRDRHVHLVLENEENTASLLRGDGLYSAQWNDDIHHALHT
                     ALTGEDASYYSDFADAPVRHLGRCLAEGFAYQGQPSRHRGNERGEPSADLSPTSFVSL
                     LQNHDQVGNRAFGDRITALAPPEAVRAAATIYLLAPQIPMLFMGEEWGAKTPFTFFCD
                     FEPDLAKLVTEGRREEFAKFPEFSDPATRDRIPDPSDEDTFAMAKLNWDEREKPEHRE
                     WLDFYRQLLALRREHVVPRLKGISGDEAVYRLVGERGLRVQWTLGDGSLLTLLANLGS
                     KSLGGFEQEVGSLLFATEDVTGDERDLPAWSAAWYLREAGESG"
     gene            1305..>3421
                     /gene="treY"
                     /locus_tag="VFJ72_04755"
     CDS             1305..>3421
                     /gene="treY"
                     /locus_tag="VFJ72_04755"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET7478809.1"
                     /translation="MRVPRTTYRLQLNKDFTFADAAALVPYLSELGISDLYASPYMKA
                     RPGSTHGYDVIDHNALNPEIGSDEDYERLISALRDHGMGQLLDIVPNHMGVGSDNPWW
                     TDVLEHGPASPRARYFDIDWYPTNRPGLHGKVLLPVLGDHYRAVLENGELKLSFDAGE
                     GSLAVGYYEHHCPLDPQSYPMVLEGLEGLPDDEHALELESLVTAFGNLPGQSATDEEA
                     VAERVRDAAINQGRLARLCAESAEIGRAIERRVREINGNVDDPESFEGLHGILEEQAY
                     RLVFWRVASDEINYRRFFAINDLAGVRVEDEEVFEATHQLILQLVAEGKVQGLRIDHP
                     DGLYDPAGYFRRLQQAAGAALGSESPDEGEPLYVLVEKILAHHEHLPEDWPVAGTTGY
                     EFTNLVNGLFVDAAGEAGMDRAYRRFIGRRVDFEELLYGCKKLIMKEALASELNVLSR
                     RLLSISGGFAGRRSHDFTINALRDALTEVVAEFPVYRTYASEGGLSEQDARYVEWAVA
                     RARKRSTAADTTVFDFIRDVLLLNEDGPEEHRASVLAFVMKFQQYTGPVMAKGMEDTA
                     LYIYNRLSSLNEVGGEPERFAVSPAAFHHLNAERAKHWPHAMLASSTHDTKRGEDVRA
                     RINVLSEMPSEWRAALVRWGRLNRSRRREVDGDLAPSRNDEYLLYQTLLGAWPLGEMS
                     EDALADFTGRIKAYMEKAIREAQV"
BASE COUNT          610 a         1122 c         1148 g          541 t
ORIGIN      
        1 gcgccggact cctcctacgg cacgccggat gacttgaaag gtctgatcca ggccgcgcac
       61 cagaagggac ttcaggtctt cctggacgtt gtctacaacc acttcggccc ggaggggaac
      121 tacctcggcg tctacgcgcc ggacttcttt accgggcggc acgagacgcc ctggggcgcg
      181 gcgatcaacg tcgatgggga gggcagcgag catgtccggg agttctttat acacaacgcg
      241 ctgtactggc tggaggagta cggctttgac ggcctgcgcc tcgatgccgt ccacgcgatc
      301 atcgacgact cgccaaagca cttgctcgtc gagctggcgg agcgcgtcca cgaaggaccg
      361 agccgggacc gccacgtaca cctggtcctt gagaacgagg agaacacggc ctcgctcctg
      421 cgcggcgatg gactctacag cgcgcagtgg aacgacgaca tccaccacgc gctgcacacg
      481 gcgctgacgg gcgaggatgc gtcgtactac tcggatttcg cggatgctcc ggtgcggcat
      541 ctcgggcgct gcctcgcgga gggtttcgcc taccagggtc agccgtcgcg ccaccggggg
      601 aatgaacgcg gagaaccgag cgcggaccta tcgccgacgt cgtttgtttc gctgcttcag
      661 aaccacgatc aggtcggcaa ccgggccttc ggcgaccgta tcaccgccct ggctccgccg
      721 gaagccgttc gagcggcggc tactatctac ctgctcgccc cgcagatccc gatgctgttc
      781 atgggcgagg agtggggtgc gaagacgccg ttcacgttct tctgcgactt cgagccggac
      841 ctggcgaagc tcgttaccga gggacgccgc gaggagttcg ctaagttccc cgagttctca
      901 gaccctgcca cccgcgaccg catccccgac ccgagcgacg aggatacctt cgccatggcg
      961 aagctgaact gggatgagag agagaagccg gagcatcgcg agtggctgga cttctaccgg
     1021 caactcctcg cgctgcggcg cgagcacgtc gtgccgcgcc tcaagggcat ctccggcgat
     1081 gaggcggtgt accggctcgt cggggagcgg ggtctgcggg tgcagtggac gctcggggac
     1141 ggctcgctgc tcacgctgct ggcgaacctg ggatcgaaat cgctgggcgg gttcgagcag
     1201 gaagtaggga gcctgctgtt cgctacggag gacgtgaccg gggacgaacg cgatctgccg
     1261 gcgtggtcgg cggcgtggta tctccgggaa gcgggggaga gcggttgagg gttccccgca
     1321 ccacctaccg gctccaactc aacaaagact tcactttcgc cgatgcagcg gcgctggtgc
     1381 cgtatctctc ggaactgggc atcagcgacc tgtacgcctc accgtacatg aaggcccggc
     1441 ccggcagcac gcacggctac gacgtaatag accacaacgc gctgaacccg gagatcggct
     1501 cggatgagga ctatgagcgt ctgatctccg cgctccgcga ccacgggatg ggccagctcc
     1561 tggacatcgt gccgaaccac atgggcgtcg gttcggacaa cccctggtgg acggacgtgc
     1621 tggaacacgg cccggcctcg ccccgcgccc gctacttcga catagactgg tacccgacca
     1681 accgccccgg tctgcacgga aaggtcctcc tgccggtact cggcgaccac taccgcgccg
     1741 tgctggagaa cggggaattg aagctctcct tcgacgcggg cgaaggctct ttagccgtgg
     1801 gctactacga gcaccattgt ccgctggacc cgcagagcta cccgatggtg ctggagggac
     1861 tggaggggtt gcccgacgac gagcacgcgc tggagcttga gagtctcgtc accgccttcg
     1921 ggaacctgcc gggccagagc gccacggacg aggaggccgt cgccgagcgg gtgcgcgatg
     1981 ccgccatcaa ccagggaagg ctcgcgaggc tgtgcgcgga gtcggcggag atagggcgcg
     2041 ccatcgagcg gcgggtacgg gagatcaacg gtaacgtgga tgacccggag agcttcgagg
     2101 ggttgcacgg gattctggag gagcaggcgt accggctggt gttctggagg gtggctagcg
     2161 atgagatcaa ctaccgcagg ttcttcgcca tcaacgacct ggcgggcgtg cgggtcgagg
     2221 acgaggaggt cttcgaggcg acgcaccaac tgatcctgca actcgtcgcg gagggcaagg
     2281 tccagggcct ccgcatagat caccccgacg gactctacga cccggccggc tacttccggc
     2341 gtctccaaca ggcggcggga gcggcgctcg gctccgaaag cccggacgag ggagagccgc
     2401 tgtacgtgct ggtggagaag atactcgccc accacgagca cctcccggaa gactggcccg
     2461 tagccgggac cactggctac gagttcacca acctggtaaa cggcctcttc gtggacgcgg
     2521 cgggcgaggc ggggatggac cgcgcctacc ggcgcttcat cgggcggcgg gtggatttcg
     2581 aggagctgct gtacgggtgc aagaagctga tcatgaagga ggcgctggcg agcgagctga
     2641 acgtcctctc ccggcgtctc ttgagtatct ccggcggctt cgccggacgg cgctcccacg
     2701 acttcaccat aaacgccctg cgcgacgccc tgaccgaggt cgtcgccgag ttccccgtct
     2761 accggaccta cgcttccgaa ggaggtctct ccgagcagga cgcgcgctac gtcgagtggg
     2821 ccgtcgcccg cgcccgcaag cgctcgaccg ccgccgacac caccgtcttc gactttatcc
     2881 gcgacgtgct gctcctgaac gaggacggac ccgaggagca ccgcgcctcc gtgctcgcct
     2941 tcgtgatgaa gttccagcag tacacggggc cggtgatggc taagggtatg gaggataccg
     3001 ccctctacat ctacaaccgc ctctcctccc tgaacgaggt cggcggcgag cccgagcgct
     3061 tcgcggtctc gcccgcggcc ttccaccacc tcaacgccga acgtgctaaa cactggccgc
     3121 acgccatgct cgcctcgtcc acccacgata ccaagcgcgg cgaggacgtg cgcgcccgga
     3181 taaacgtcct ctccgagatg ccttcggagt ggcgcgcggc gctcgtccgc tggggccgcc
     3241 tcaaccgctc ccgccgccgc gaggtggacg gagatcttgc ccccagccgc aacgacgagt
     3301 acctcctgta ccagaccctc ctcggcgcgt ggcccctggg agaaatgagc gaagacgccc
     3361 tcgcggactt cacggggcgc ataaaggcgt acatggagaa ggccattaga gaggcgcagg
     3421 t
//
LOCUS       DASSMB010000071         5498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_16946, whole genome shotgun sequence.
ACCESSION   DASSMB010000071 DASSMB010000000
VERSION     DASSMB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5498
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16946"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(961..1033)
                     /locus_tag="VFJ72_04760"
     tRNA            complement(961..1033)
                     /locus_tag="VFJ72_04760"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(998..1000),aa:Arg,seq:cct)
     gene            1344..2216
                     /locus_tag="VFJ72_04765"
     CDS             1344..2216
                     /locus_tag="VFJ72_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7478810.1"
                     /translation="MQTVILALLIGMISFGCSGAGSSGKTLRIGIQEWDENVAISNLT
                     KVLLEEDLGYDKVELQVLPLPDVFEGVGDGDLDAFQDVWMPNHKDRLSEVSAGVDHLP
                     AWFRGKTAYGIAVPDYMSDVKSLNDLNRAGTDMIFGIEPTAAFMPKIEKKVIPGYDPD
                     IKLVASSTPAMLAELKNAYDEKEPIIFLGWSPHWMNATYKFRYLKDPKGLEKPFSDPS
                     KLSTIVRKDLKEDDPVAYQLIKSISLTEEQVNALEAEINASGDPVKGVESWLADNRSV
                     VTPWIDRAEKTQES"
     gene            2284..3183
                     /locus_tag="VFJ72_04770"
     CDS             2284..3183
                     /locus_tag="VFJ72_04770"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7478811.1"
                     /translation="MSFATVFLAALVVLLAGCGSGEASKPADASGLKLRIGYAGFGED
                     KAVSELTKVLLEERMGHGEVELRRVGIHEAYRDVADRKLDAFQGVRMPGQQKEFEKVS
                     DRTALFNSWIIGTTRASLAVPSYMKVRNPEQLDQDAAAHVLTIRPDASPVGEIPKRAL
                     ADYGLRVEREYRDAPAMLDEVDRLYKEKKPFVFIAWTPHWMNDEYDINYLEDPKGSLS
                     EITRPGSLHMLVRKDLQEKDPLAHALMDAILLTETEATNLQVSIRQAKSPKQGVHNWI
                     KEHDNDKLVKGWVESARKRVADG"
     gene            3803..5029
                     /locus_tag="VFJ72_04775"
     CDS             3803..5029
                     /locus_tag="VFJ72_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET7478812.1"
                     /translation="MTEMDTTAAELAKGAPRILLYTRLRRSPYFYSSLRHGPVMYSTY
                     NHHYHPRHYGDPVEEYWELLNGVTLWDVGGAERQVEISGPDAFEFTNMLVPRDLNKCA
                     VGQCKYVFLTAEDGGIVNDPVLLRLDENHFWLSLADSDALLWARGLAYNSGLDVQIRE
                     PDVGPMQVQGPKSRDVMVDLFGESILEIPYYFMEHRELDGMEVVVSRTGYTSELGYEI
                     YLKNASRDGDRLWSAVWEAGQPHGMRVIGPCHIRRIEGGILAYGCDMTLDTNPYEVDY
                     GYEWMVDLDQEADFVGKEALRRIREEGIKRKLVGVEIEGEQLGSYNDGSMIDYFPVYD
                     GEERIGQVTSACYSPRLEKNIGYAMVPIEYIEYGTGLLVETPHGRRDAVVVRRPFVDP
                     EKEIPKTDPRKQAAAS"
     gene            5043..>5498
                     /locus_tag="VFJ72_04780"
     CDS             5043..>5498
                     /locus_tag="VFJ72_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478813.1"
                     /translation="MRREMAEPSRDRAETVRGDYPRLPDAARSALMRPRFEVVPVEGA
                     LEQAALLPPRAEVSITCSPTLGIENTLALAEKLSDRGFRAVPHVSARLVTGEAHLEEI
                     LRRLDDSRLRDVFVVGGDVKEPAGPFASGLELLRAMSRLGHGIQRIGIPA"
BASE COUNT         1078 a         1731 c         1807 g          882 t
ORIGIN      
        1 ccggcgcttc ggcgagaccc tcgaagggcg ggccgcggac ctgagcccgg aaggcgcgct
       61 gctgctggag acgcggcgcg ggacagtgga gctgttcgag ggagagatcg aacacctccg
      121 ccagggcaac gccatatgaa ggtcgcctgc gcccagtacg ccatccggga cggagacgcc
      181 gcctccaacc tccagcgctc tctcgaatac gtccaccgcg cggcggatca cggcgcagac
      241 ctcgtcgtcc tgccggagct cgccaactcc ggctgtccgg cctccagaga cgaagctctg
      301 aagctagccg agccggtgcc ggacggtccc acggcgggag cctggcgcgg cgcggcggcg
      361 gagcgcggca tccacatcgt cggcggcgtc ctggagcggg agggagacac gctgcgcaac
      421 tccgccctca tcatcgggcc gggcattctc gggcgatacc gcaagaccca tctctgggac
      481 cgggagaaga tgatctacga gcccggagac gcgcttccgg tcttcgacac gccgctcggc
      541 aggatcggcg tcctcatctg ctacgacgcc tggtttccgg aggccgcccg cgctctggcc
      601 gtgcgcggcg cggacatgat ctgcatcccc tccaacgccc ccgatgactg ggtccccgaa
      661 gagcagcgaa gaggcggcct cacaatgctc gatgtccatg ccatcgcgca cgccaacgcg
      721 aaccgcgcct tcgtggccgt agccaacaga gtaggtgacg gctacctggg ccgtagctgc
      781 gccgtggaca ccaccggcgg catactggcc ttcgccagcg gtacggagga ggccctgatc
      841 ctggccgaca cggaacctgc tcgcgcccgc cacgccaaac aactaacccg taactcccac
      901 gccctcggag acaggaatat cgctgcatac gaggatgacg ggagcgggcc ggccgcggag
      961 tgcgcctgga aggattcgaa cctccgacac agggtttagg aaacccttgc tctatcccct
     1021 gagctacagg cgcgaggggc cgctagttta tcgcaggagt ccggcgcgta ccagcgtccg
     1081 cggggcggca tcgagaggat gaaatattgt tgaatatacg tgtggaagtc cgtatcatga
     1141 gtctcgcggc cgggcagaga cggtttgcaa gcattctttt atccggtcgc gggctgggct
     1201 gtgcgtgatc tccgcaagat cctgccgcgt aggacattta aggagtgaat acggaagctg
     1261 acaatatgca ccggaagtcc ccgcttctcg cgcaaatctc atggttccgc caacctctta
     1321 tcggcggcgc tccgcgaagg gttttgcaga cggtcatcct cgcgctcctt atcgggatga
     1381 tctcgttcgg ttgctccggg gcaggttcct ccggaaagac gctcaggatc ggcatccagg
     1441 agtgggacga gaacgtggcc atctccaacc tgacgaaggt gctcctcgaa gaggatctcg
     1501 gctacgacaa ggtggagttg caggtcctgc cgctcccgga cgtcttcgag ggggtcggag
     1561 atggggatct agacgccttc caggacgtct ggatgccgaa ccacaaggac cgtctgagcg
     1621 aggtctccgc cggcgtggat catctgcccg cgtggttcag ggggaagacg gcctacggaa
     1681 tcgccgtgcc cgactacatg agcgacgtca agagcctcaa tgacctgaac agggccggga
     1741 cggacatgat cttcggcatc gagcccacgg cggctttcat gcccaagatc gagaagaagg
     1801 tcatccccgg ctacgacccg gacataaagc ttgttgcctc cagtacgccc gcgatgctcg
     1861 ccgaactgaa gaacgcctac gacgagaagg agccgataat attcctcggc tggtcgccac
     1921 actggatgaa cgccacttat aagttccgct acctgaagga cccgaagggc ctggagaaac
     1981 ctttcagcga cccctccaag ctctctacca tcgtccgtaa agacctgaag gaggacgatc
     2041 cggtggcgta ccagctcata aagtcaatct cgctcaccga ggagcaggtc aacgccctgg
     2101 aggcggagat caacgcgtcc ggagaccccg taaagggcgt cgagtcctgg ctcgcggaca
     2161 accggagcgt cgtgacgccc tggatagaca gagccgagaa gacccaggag tcgtgaggcg
     2221 aaggcctcgc ccacattgag gcgcgaaggc tcggaccggc ggtttactac ccgtgcggct
     2281 accgtgagtt tcgcgaccgt tttcctcgcc gcgctggtgg tcctgctggc cggttgcggc
     2341 tccggcgaag cgtcgaagcc cgctgacgct tccgggctga agctgaggat cggctacgcc
     2401 gggttcggcg aggataaggc cgtctccgag cttacgaaag tcctgctaga ggagcggatg
     2461 ggccacggag aggtcgagct tcggcgcgtc gggatccacg aggcctaccg ggacgtcgcc
     2521 gacaggaagc tggacgcctt ccagggcgtg cggatgccgg gccagcagaa agagttcgag
     2581 aaagtctcgg accgcaccgc gctgttcaac tcctggatca tcggtacgac ccgcgccagc
     2641 ctcgccgtcc cgtcgtacat gaaggtgcgg aaccccgaac agctagacca ggacgccgcc
     2701 gcccacgtgc tcaccatccg ccccgacgct tccccggtgg gcgagatacc gaagcgggcg
     2761 ctcgcggact acgggctgcg cgtggagcgc gagtaccggg acgcgccagc catgctggac
     2821 gaggtggaca ggctctacaa ggagaagaaa cctttcgtct tcatcgcctg gacgccgcac
     2881 tggatgaacg acgagtacga cataaactac ctggaggacc cgaaaggctc gctctcggag
     2941 ataacccgcc ccggttccct gcacatgctg gtacgtaagg acctgcaaga gaaagacccc
     3001 ctggcccacg ccctcatgga cgccatcctg ctgaccgaga ccgaggccac caacctgcag
     3061 gtctccatac ggcaggcgaa gagcccgaag cagggcgtcc acaactggat aaaggagcac
     3121 gacaacgaca agctcgtcaa gggctgggtc gagtccgccc gcaagagggt agccgacggc
     3181 taacgaatag cgagttcaga tcgcatttca gcgtcctcta ctccgcccat gcgggattca
     3241 tgggctctcc agaaggtcgc aactctagcg cccgcgccag tggaagaccg ccccattgcg
     3301 catcagttgg ccgggacggc gacgcaccac tccgctcact tacatgtaac tggatgacgc
     3361 ccgcgtagaa ctcccagaat taccatccca ggcgtcacac cttacccgca cacaccatcc
     3421 cagcgctatc caggaatccc acccttgacc tcgaaagctg catatgatat ttgttctgtg
     3481 tatgggaaag cgattcgtaa tacgcaacaa aaatgagttg cagtggtgtc cggtttcgtt
     3541 accgtcgtcc tgatgtagtc gaggggagac ctccgtgaac gatagaggcg ggatctttgg
     3601 ggacggcgcc gatgggaggt agcgcgggct ggtatctggt gaggttcggc tggaaggacg
     3661 tggtcctgat cggaagtggc tatccggacc ggcggcgagg cggatgcaag tccaggagag
     3721 tggggatgtg gcgaagtagt tggccggtgt tggttggcgc tccggcccgg cgggccgggg
     3781 catcggatcg gagaggagcg tcatgaccga gatggacact accgcggcgg agctggcgaa
     3841 aggggcgccg aggatactgc tgtacacgag gctcaggaga agcccctact tctactcctc
     3901 gctcaggcat ggtccggtga tgtacagcac ctacaaccac cactaccacc cgaggcacta
     3961 cggtgacccg gtcgaggaat actgggagct tctgaacggc gtcacgctct gggacgtcgg
     4021 gggagcggag cggcaggtcg agatctccgg tcccgacgcc ttcgagttca cgaacatgct
     4081 cgtaccgcgc gacctgaaca agtgcgccgt cgggcagtgc aagtacgtgt ttctcacggc
     4141 ggaggatggc ggcatcgtca acgaccccgt gctgctgcgc ctcgacgaga accacttctg
     4201 gctctcgctg gccgacagcg acgcgttgtt gtgggcgaga gggctcgcct acaactccgg
     4261 cctcgacgtg cagatccggg agcccgacgt cggcccgatg caggtgcagg gtccgaagtc
     4321 cagggacgtg atggtggacc tcttcggcga gagcatcctg gagatcccgt actacttcat
     4381 ggagcacagg gaactcgacg ggatggaggt cgtcgtctcg cgcaccggct acacctcgga
     4441 gctggggtat gagatctacc tgaagaacgc cagccgcgac ggtgaccggc tgtggagcgc
     4501 ggtctgggaa gccggacagc cgcacgggat gagagtcata ggaccctgcc acatacgcag
     4561 gatcgagggc ggcatcctgg cctacggttg cgacatgacc ctcgacacca acccctacga
     4621 ggtggattac ggctacgagt ggatggttga cctcgaccag gaggccgact tcgtgggcaa
     4681 ggaggccctc cggcgcatca gggaagaggg aataaagcgc aagcttgtcg gcgtcgagat
     4741 cgagggcgag caactcggct cctacaacga cggctccatg atcgactact tcccagtcta
     4801 tgacggcgag gagcggatcg gccaggtgac ctccgcctgc tactcgccgc gcctggagaa
     4861 gaacatcggc tacgcgatgg tcccgatcga gtacatcgag tacggtaccg ggcttctggt
     4921 cgagacgccc cacggccggc gggacgccgt ggtcgtgcgg cggcccttcg tggacccgga
     4981 gaaggagatc ccgaagaccg accccaggaa gcaagccgcc gcgagctagc ggaggccggg
     5041 ccgtgaggcg ggagatggcg gagccgtccc gagaccgcgc agagaccgtt cgcggcgatt
     5101 atccgcgcct cccggatgcc gcgcgctcgg cgctgatgcg gccccggttt gaggtcgtcc
     5161 cggtagaggg cgcgctggag caggcggcgc tactcccgcc ccgcgcggag gtctcgatca
     5221 cgtgctcgcc taccctgggc atcgagaaca cgctcgcact cgccgagaag ctctcggacc
     5281 ggggcttccg ggccgtgccg cacgtctccg cgcgtctcgt caccggcgaa gctcacctcg
     5341 aggagatcct gcgccgtctc gacgacagcc ggctgagaga cgtcttcgtc gtgggcggtg
     5401 acgtgaagga gcccgccggc ccgttcgcca gcggcctgga gttgctccgg gcgatgtcgc
     5461 ggctcgggca cggcatacag aggataggca tacccgcc
//
LOCUS       DASSMB010000072        17422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1030741, whole genome shotgun sequence.
ACCESSION   DASSMB010000072 DASSMB010000000
VERSION     DASSMB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 17422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17422
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030741"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFJ72_04785"
     CDS             <1..209
                     /locus_tag="VFJ72_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HET7478814.1"
                     /translation="ALMGGDVAQAVREIREKGIIVLSLNMPGSVPDAADMVVTDPVQC
                     GVMAVMAVSSSGRFDIRRQRGRRY"
     gene            complement(613..1425)
                     /locus_tag="VFJ72_04790"
     CDS             complement(613..1425)
                     /locus_tag="VFJ72_04790"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HET7478815.1"
                     /translation="MGYVNNEGRPVKDQVVLPVAHFFNHQTHPSGPGPVSDHPPAGLE
                     RKPDLRAMVNVPELQFWFEFGSTYSYLSAARIEDAAASAGVTVSWEPFLLGPIFKDQG
                     WDDSPFNVYPAKGQYMWRDLERLCAGYGLPFAKPSRFPRSGLLAARVACLAKSSSEPW
                     LPDFARAIYRANFAEDREIGDSAVVCSILDSLGQPGAQTLERSEAQEIKTLLREQTRR
                     AVEIGIFGAPSFVVGDELFWGNDRLEDALAWAGSRTEDDKGSRRRKGRRNGT"
     gene            complement(1755..2999)
                     /locus_tag="VFJ72_04795"
     CDS             complement(1755..2999)
                     /locus_tag="VFJ72_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET7478816.1"
                     /translation="MSGPRYLTAREAAGELGVSLKTLYAYVSRGLVRSEAGGGKRRGR
                     RYRSEDVRRLKERKEQRRDPGRAAERALDWGMPVMESAITLISDGRMYYRGRDAVVLS
                     GDHSLEQVAELIWTGSMTEGDRGRLPDLFRVSAGPGVPEHPWLVDPGFGLRSPTEVFG
                     MALQAAAAADPAAYDLRGAAVARTGARILRLLAATATAGGQGAGNTVARTLQRRWAPR
                     DAEAADLLDMALVLCADHELNASSFTARCVASAGATPYAVVVAGLAALGGVKHGGITE
                     RIEAFLAETEAAGDARRVVTSRLRRGETVPGFGHQLYPDGDPRGEALLAALEEKYPGS
                     EASKLAREAAEAAGDLMGEKPNVDFALTVLARVLDLPPGGALALFALGRTVGWVGHAI
                     EQYEVDRIIRPRARYVGEQPTE"
     gene            3328..4464
                     /locus_tag="VFJ72_04800"
     CDS             3328..4464
                     /locus_tag="VFJ72_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HET7478817.1"
                     /translation="MNHRTAVDFAPGLEGVVATRTRLSMVDGSAGRLVIAGFPVEELA
                     GMATFEETLYLLWNGSLPDEEQLAVLRGDLAVRRALPKVAGDVLKEAAAARMPPMDAL
                     RMAAGTLGTGDGDDRLDALRLVAAFPTIAAAYWRFSQGWEPIEPDPALSHAANYLYML
                     SGETPTAEQVRALETYLNTVSDHGMNASTFAARVIISTRSDVISAVVGAVGALKGPLH
                     GGAPGPALDVVFEIGEAGNAEPVLRAKLESGERLMGFGHRVYRVRDPRADVLAAAAER
                     LYATDADRDLYRLALDVEKTAVRLLEEYKPGRNLDTNVEFYTALLLHGLGLPTELFTP
                     TFAVGRVAGWTAHCFEQRELDRIIRPQSEYVGPDGLHWAPLEER"
     gene            complement(4624..5457)
                     /locus_tag="VFJ72_04805"
     CDS             complement(4624..5457)
                     /locus_tag="VFJ72_04805"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET7478818.1"
                     /translation="MNAQRKARKIVQTGRHHAGQNRRALGRIVLLAILVGGLLIGMGP
                     VMGPAGAATASDTLQDVRFGDHGTYERAVLDLGWDHAPGDVAPVYTWRYRNGDWIVRI
                     NLPAVESTLKTDGPALGLGISRYYVVRRADDTLAVDLRLRKPAGTVKVFSLDHPARVV
                     VDVRPGGLPLYPEPAECKTAVITAPRLSYLVGPDTFKVSGYGRPFEAQGVWRLKNSAG
                     RVVRQGAYTTNDWDSAWGSFGFEVTYPSTLAGHTGMLQVGDYSPEDGHFRGASVALRF
                     E"
     gene            5695..6795
                     /locus_tag="VFJ72_04810"
     CDS             5695..6795
                     /locus_tag="VFJ72_04810"
                     /inference="COORDINATES: protein motif:HMM:NF017634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HET7478819.1"
                     /translation="MAAQGYDVVFVGGGLSTMLLLSELRFVLPERVAVIEPSPPLERP
                     SVHWSYWSREQTLYDRFAIGVWRRARVGDAPPEPITPFVLRLVRSTDVFAHIAALLAD
                     TPIDWLRTSAREISRRNDDLYEISTDAGTVHARWVFDSVCDIAPTFPSQGEPRGVLSG
                     TGLRVTADRPVFDAATATLFDPLDEQSFAYLLPLSPIEALLESASFGSDARREDRRPL
                     LQYLQARHPKAHFTVTQAEWGAIPLGFAPPRTAGPRHILIGAKRGLVKPSAGYGVVRI
                     ARESEHLSRLWREDPLLPTGWRAPWRWRLLDKGFLLLAAQDPRLPMALLRRVMNAVPL
                     EESLRFIDEELPPRKMAAFLPSALLAVLQRPR"
     gene            7012..7650
                     /locus_tag="VFJ72_04815"
     CDS             7012..7650
                     /locus_tag="VFJ72_04815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478820.1"
                     /translation="MMASATRPDSGVDRSAGSGGDCGVIDLLDGVRVSYRPIRAEDAA
                     ALQRFHLRLSRRSVYLRFFGAKPELSDRKAGYFARLDGVNQFALVALDPDRPGEIVAI
                     VSYYREGSSDRAEYAAVVEDGWQGRGLGLELTRRLIQAALKRGVACFTGVVLPENARM
                     LSLLRDLNLPEHLRLGDGLEYVEIELAPTTKKHPSMTPSLESGGGIRCLEIP"
     gene            7892..8905
                     /locus_tag="VFJ72_04820"
     CDS             7892..8905
                     /locus_tag="VFJ72_04820"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET7478821.1"
                     /translation="MPGDLLLLSEGDRLSADARLIDGSVEVDVSPLTGESQPVVRSAT
                     RSRSASSPLESDDLVFSGTLVTAGEAESVVYTTGMGTQLGRIAALTQRVRGETSPLQR
                     QVNGLSVLQLGNFAFGHPSRITARVRMGKGEVVDIEREVELGGPLHSKGVLILSGFLG
                     ARYAAERPLSLSASLVFEQSYGGVEGDSASSAELYALLSAISGLPIRQSLAVTGSVNQ
                     HGQVQAIGGVNEKIEGFFDVCRARGLTGEQGVLILASNVKYLMLREDVVSAVEQGRFH
                     VYPVETVDEGIEVLTGTPAGERDAAGKYPEGSVNGLVEARLAALTEKQARFGRTFQEG
                     GPE"
     gene            8902..9516
                     /locus_tag="VFJ72_04825"
     CDS             8902..9516
                     /locus_tag="VFJ72_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7478822.1"
                     /translation="MSERGLALRRILVALETAASKARINWSFQVARGTVATEMLAAAS
                     EADLLALGKSGWTAVTEGRLGSTVRAIVSAGSGPVLILQPGARLGTPVLAVNDGSSAA
                     RKALEFAARLLPEEDGGLSVLIAANDAETARTLQKQAGEWAERRRINVRFRTLLGGRV
                     PELVRALGTEDAGLLVLPGPFLSATAGALPAILSRIERPALLVW"
     gene            9822..10226
                     /locus_tag="VFJ72_04830"
     CDS             9822..10226
                     /locus_tag="VFJ72_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET7478823.1"
                     /translation="MQSEMDRLFGETFGRLPRMAAETEWAPAVDVTTEDGDMVIRAEL
                     PGVKREDVDVTLSGGVLTISGERKEEQERKDAGYLVRERRYGSFRRAMTLPEGTNESK
                     VSASFEDGVLEVKVRDGASVVEERPRRIEIEE"
     gene            10358..10822
                     /locus_tag="VFJ72_04835"
     CDS             10358..10822
                     /locus_tag="VFJ72_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7478824.1"
                     /translation="MSDLPARILVATDGSADAEMAGRRASAMARALGSELHMVYVVPV
                     TEPYSPYSEGDGERPGTYEDRREARALLAEQVGLVEEAGGKVEKSYIRVGEPDAEVVS
                     LAEEIGANMIVTGSRGLGRLRRPIGSTSSSIAAHAHCPVLVVRRESESGVKR"
     gene            10819..11712
                     /locus_tag="VFJ72_04840"
     CDS             10819..11712
                     /locus_tag="VFJ72_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7478825.1"
                     /translation="MNEVLRKLLLAIDGSEDAALAARAAVDLADRTGAGLHVVHAWQP
                     MYPYVNYPASVSTDFVRIYEERAGSLLGVQVDQIEAEGGAVEESHLVMGAPVEAILEL
                     CGQIEPDLIVVGSRGMGPIRRVLTGSVSEGVVHHAGCPVLVVRGGEGGWPPSRILIGD
                     DGSGDAERAARLAARLGALYGVGAALVRAYENPPSPIGGWSAEDRRRLDETLVEEEAA
                     LDRRADEVEKVLGSRPETRMVEGDATMALLEIAGEEEKTLFAVGSRGLGLFSRLRLGS
                     VSTKVLRTARGPVLVCTHHSG"
     gene            11872..12366
                     /locus_tag="VFJ72_04845"
     CDS             11872..12366
                     /locus_tag="VFJ72_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7478826.1"
                     /translation="MNTSGAKSPYTNLSRRDRVALSLEHEGNKRLRNLSTVLYRLTGG
                     RFAPHDRDVILLTTRGRKSGREHTVLLQSFPDGDDMILVAANSGRSSHPDWFYNLKAE
                     PAARVEIEDRTLMVRAGQVPEDEAEALWPGILRHAPTYARYRRATDRAIPLVRLVPIG
                     SDEG"
     gene            12648..13454
                     /locus_tag="VFJ72_04850"
     CDS             12648..13454
                     /locus_tag="VFJ72_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7478827.1"
                     /translation="MKKRRSYRVVPFTMNRRMVAASASVGRERNNIHAMIEVDVTEPR
                     RLMREHRERTGERLSLTAFVVTCLARAIAENPNLNSFRKGRKLILLDDVTVSVLVERE
                     IAGEKVPEPFGIRAAQTKTYRRIHDEIRATQRHGEDGLGGLSGISWVRFIPGFLLRAF
                     VRVASRNIHMMDRFGAVGVTAVGMFGGNEALWLIPLSGATVAVTVGGIVERPSASGGR
                     IETREHLCLTVTFDHDIVDGVPAARFLKRFSELLMISEPLGDTIVTAAVE"
     gene            13466..14473
                     /locus_tag="VFJ72_04855"
     CDS             13466..14473
                     /locus_tag="VFJ72_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478828.1"
                     /translation="MNTLPTKRNLTLAYRLSLVVAALMAIASIAGILLGSAGLYGADP
                     KLALGVTEAEAGLLLPGLLGQDLFNLVVGVPLLLGSMWLARRGSLIGLLLWPGMLFYA
                     LYWYVLYAVGAPFSVLFLLYVPLVTLSAYATIALVSSIDGESVRRLTEAVPARTIGGI
                     LVVLALLTLAQDATGAFVTALAGNAPVDPAARHVWISDLALQAPAVLAVGALLWRRHP
                     LGYVTAAGLLFQYGLTPIGLVAGMALQAALSGSPLDAGTSGVLLVFSGVCFVSLVFFV
                     RAAARRRLPSPGTETTGTSTGMSTGYSGAGDERGVPDVADDRMEDVPAVKRSWRGGRE
                     G"
     gene            14470..15225
                     /locus_tag="VFJ72_04860"
     CDS             14470..15225
                     /locus_tag="VFJ72_04860"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HET7478829.1"
                     /translation="MRAATGTSRSSGGAATGPDLSWRGLYRAGALSAVLYVVLIVVPV
                     VLLFAAPQPPLSGGDEILRYIAENKAVYVIELVSFVGLSLPAIVVFLALYVALRHLDR
                     SYAALGAVVGIASEIVALAYNSSPPSLNGGLLYLSDQYAAAATDAQRAALATAAEGLV
                     AVSNAVNAAGILTAIGILIISLVMLKGAFPRGVAYLGIAAGAVGIVSEALRDMIGAGY
                     FVYGLLLPSWFIAVGWTLFRLAGGGSIRMKRAG"
     gene            15261..15461
                     /locus_tag="VFJ72_04865"
     CDS             15261..15461
                     /locus_tag="VFJ72_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478830.1"
                     /translation="MWWADLLWGLWNGLTAWIVFIVHVFGGWEQFPFYNAARGGNWYD
                     FGFLLGAGSPLLGASRGRSRAT"
     gene            15715..16926
                     /locus_tag="VFJ72_04870"
     CDS             15715..16926
                     /locus_tag="VFJ72_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HET7478831.1"
                     /translation="MNANSAAGPQPGAYRALVLATLAFALCFSVWGLVAPLAPQFQEL
                     YKLSSTQISVVIATPVLLGSLLRIPIGLITDRFGGRAVFAVLMLVLIVPVMFIGLLGG
                     SFGGLIFWGFFLGLAGSSFAVGIPFVSKWFPPHMQGLALGIYGMGNIGTAIAAYTAPS
                     IAASYGWQWAFWVFIIPLVAMAAVFWVMGRDAPSTGPRPSVTAGIALFREEVMPWVLS
                     LFYFLTFGGFVALGIYLPKLLVDLFGFTPSGAGLRAAGFVVLATLSRPVGGWLADRIG
                     GSRTLMVIFAVLPFMALILSFQPGIVLFTFAALSSAVLLGLGNGAVFKLVAEYYPTKT
                     GAVTGVVGAAGGLGGFFPPLVLGIVRDATGSYAIGFVLLALFAVGCLLVNALFVRRHP
                     SGTGVAQMRGA"
     gene            16931..>17422
                     /locus_tag="VFJ72_04875"
     CDS             16931..>17422
                     /locus_tag="VFJ72_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HET7478832.1"
                     /translation="MARATNPLLRDLQYFRRGEKRAGGWSELNARDREWEYAYRQRWQ
                     HDKIVRSTHGVNCTGSCSWKVYVKDGIITWETQQTDYPSNGPDSPEYEPRGCPRGASF
                     SWYTYSPIRIKYPYVRGVLMQMYREAKAQAGDPVEAWASIVEDPEKARAYKAQRGKGG
                     LVRA"
BASE COUNT         2873 a         5511 c         5842 g         3196 t
ORIGIN      
        1 gagcgctcat gggcggcgac gtcgcccagg cagtccgcga gatccgggag aaagggatca
       61 tcgtcctctc cctcaacatg cccggcagcg tccccgacgc cgctgacatg gtggtgaccg
      121 atcctgtcca gtgcggcgtg atggccgtga tggctgtctc gtcctccggg cgcttcgaca
      181 tccgacggca gcgaggccgg cgctactgac agcccggcgg gccgtgccgg tatcggctac
      241 gcgggatgcc cctcggatgt tcgtctatcc agggcggcca gggtcacggc ggcctcggag
      301 gtcaggacct gcgcttcgca gaggagccgt tcgccccggc cggagcaggt tgatcttgaa
      361 ctcgacagtc aaacgtagct ggcctcgcgc tatcagggcg cccgccgcgg cgccggcggt
      421 acggtccacc atcgtcgcct gcaccccggc gtgaacgtac ccgtcttact acaggtgcct
      481 ggacgagacc cgcagctctg agtcgcctca tcccggtccg gcgcccacga gccgaaaact
      541 cgacgtcggc gatgaacgct gcctgctcga acagcgcctc gacgaaggcc atgtagtcgg
      601 ggtttctggt cttcacgtac cgttccgcct cccttttcgc cggcggcttc ctttgtcgtc
      661 ttcggtacgc gaaccggccc acgccagagc gtcctccaga cggtcgttgc cccagaaaag
      721 ctcgtcacct accacgaagc tcggggcgcc gaagatcccg atctcgacgg ctcgccgcgt
      781 ctgctcccgc aggagagttt tgatctcctg cgcctccgat ctctccagtg tttgcgcgcc
      841 tggctgtccg agcgagtcga gtatcgagca gaccactgct gagtccccga tctcgcggtc
      901 ttccgcgaag ttggcccggt agatcgcccg cgcgaagtcc ggcagccacg gctcggacga
      961 cgacttcgca aggcaggcta cccgggcggc gagcagtccg ctcctcggga agcgcgacgg
     1021 ttttgcgaac gggagcccgt accccgcgca caggcgctcc aggtcccgcc acatgtattg
     1081 gcccttcgcc gggtagacgt tgaacggcga gtcgtcccat ccctggtcct tgaagatcgg
     1141 gcccaagaga aacggctccc aggagaccgt tacgcccgca gaggcggccg cgtcctctat
     1201 ccgggctgcg gagaggtacg agtacgtgct tccgaactcg aaccagaact gaagttcagg
     1261 gacgtttacc atcgctctca agtccggctt tcgttcgagt ccggcgggcg gatgatccga
     1321 tacaggtcca ggaccggagg ggtgggtctg gtggttgaag aagtgcgcca ccggcagcac
     1381 gacctgatct ttgacaggcc tgccctcgtt gttgacgtag cccaatgttt cgccgaggga
     1441 tcgatcctcg atgacggctg cgaaggcttc gatgcgcgca tcctcttcct gcctcgcttc
     1501 ccatagctcc tcgaagtcct cttacagggt agcgtagaag tctgtggatg ggacatcttc
     1561 tccggcggag cgggactgcc ggacccggcc ggccttgagg agacggtctc tatggggacc
     1621 atgcgcgaga cgtcttgcac cgggaggggt ctcctggacg tcatgacaat ttacctcttc
     1681 cactgacgct gtctcgaagg gatacaggta ccttctgatc tccgggattc tactctcctt
     1741 ctctgtcgcg aactttattc ggtaggctgc tctccgacgt agcgggcgcg cggcctgatg
     1801 atcctgtcta cttcgtactg ttcgatggca tggcccaccc agccgacggt tctgccgagg
     1861 gcgaatagcg cgagagcccc gcccggtggc aggtccagaa ccctcgccag gaccgtcagc
     1921 gcgaagtcca cgttgggttt ttcgcccatc aggtctcctg cggcctccgc cgcctccctt
     1981 gccagctttg atgcctctga gccggggtac ttttcttcca gggccgcgag tagcgcctcc
     2041 ccccgcgggt ccccgtcggg atagagctgg tgaccgaacc ccggcacggt ttctccgcgt
     2101 ctcaaacgtg atgtgaccac ccttctagca tcaccggccg cttccgtctc cgccaggaat
     2161 gcctcgattc gttcggtgat tccgccgtgc tttacgccgc cgagcgccgc cagcccggcc
     2221 accacgacgg cgtagggcgt ggccccggcg gaggcgacgc acctggccgt gaaggaggaa
     2281 gcgtttagct cgtggtccgc gcagaggacg agcgccatat ccaacaggtc cgcagcctcc
     2341 gcatcccgcg gagcccatcg gcgctgaaga gtccgggcca cggtgttccc ggcgccctgt
     2401 ccgccagcgg tcgccgtcgc tgctagcaga cgcaggatgc gggcgccggt cctggctacg
     2461 gcagcgccgc gtaggtcgta ggcggccggg tccgccgctg ccgctgcctg tagcgccatc
     2521 ccgaaaacct ccgtcgggga cctcagtccg aaccccggat ccacgagcca gggatgctcg
     2581 gggacgccgg gtccggcgga aacccggaaa agatcgggga gccttccgcg atctccttcc
     2641 gtcatgctcc ccgtccagat caactcggcg acttgctcca gggagtgatc cccggagagg
     2701 acgacggcgt cgcgtcccct gtagtacatc cgcccatccg agatcagagt gatggccgac
     2761 tccatcaccg gcatgcccca gtcgagcgct ctctcggcgg cccggccggg atcccgccgc
     2821 tgctccttgc gctccttgag cctccgcacg tcctccgaac ggtatcgccg gccgcgccgc
     2881 ttccccccgc cggcctccga cctgacgagc ccccggctca cataggcgta cagcgtcttc
     2941 aagctcaccc ccaactcccc ggccgcctcc ctggcagtga ggtagcgagg tccgctcatt
     3001 cctgccacca ctccttcgga tttttgtgaa ggtccgtagc tcaacccttt caaagtatag
     3061 ttgattgata aatcaacgtt gataaacatc tgccgctatt ctacagtgaa ggaaaccccg
     3121 gcgataagag tggcaggaag gctctacaga accttttggg caaggtctgc gtggtgatcc
     3181 aggggtgtgt cagatgtctg tgaggggcga gataggttgg cgcgggcgac ctcgttctac
     3241 ggcaccgggc aactccggtc cggggatgac ggcaacgtgt tgggtggccc gcaatgagaa
     3301 cccgatgcca aggaggtcat tttggagatg aaccatagaa ctgcggtcga tttcgcgccg
     3361 ggactcgaag gcgtggtcgc gacgaggacg cgcctcagca tggtggatgg gtcggcggga
     3421 cgcttggtca tcgccggttt ccccgtcgag gagttggcgg gcatggcgac gttcgaggag
     3481 acactgtatc tgctctggaa cggctcgttg cccgatgagg agcaactggc ggtgttgagg
     3541 ggggatctcg ccgtccgcag agcgttgccg aaggtcgccg gggacgttct caaggaggct
     3601 gctgccgcgc ggatgccgcc gatggacgct ctgcggatgg ccgccgggac gctgggtacg
     3661 ggagatggag acgaccgcct cgatgccctc cggctcgtcg ccgcgttccc gaccatagcc
     3721 gctgcttact ggcgcttctc gcaaggctgg gagccgatcg agccagatcc cgcgctctcc
     3781 catgcggcga actacctcta catgctctcc ggcgagacgc cgacggcgga gcaggtccgg
     3841 gccctcgaaa cctacctcaa caccgtctcc gaccacggca tgaacgcctc gaccttcgcg
     3901 gcgcgggtga tcatctctac ccgttcggat gtgatctcgg cggtcgtggg cgcggtcggc
     3961 gcgctcaagg gaccgttgca cggcggcgct ccgggtccgg cgctcgacgt ggtcttcgag
     4021 atcggggagg ccgggaacgc cgagcctgtc ctgcgggcga agctggagag cggcgagagg
     4081 ctaatgggtt tcggccacag ggtctaccgg gtgcgcgacc ccagggcgga cgtgctcgca
     4141 gccgcggccg agcgtctcta cgccaccgac gccgacaggg acctctaccg gctcgccctc
     4201 gatgtggaga agacggccgt gaggcttctc gaagagtaca agccggggcg caacctcgac
     4261 accaacgtcg agttctacac cgcgctattg ctgcacgggc tcggactgcc cacggaactc
     4321 ttcaccccca ccttcgccgt aggccgggtt gcgggatgga cggctcattg cttcgagcag
     4381 cgggaacttg accggatcat tcgtccgcag tccgagtatg ttggtcccga tgggctgcat
     4441 tgggcgccgc tggaagagag gtagacccgc aaaaacagag agcgtggcag ccgtgagggc
     4501 accgtcttaa cgccgtccga acttcgggtt tcgtctaccg ggacttgagg aatcggggga
     4561 gtgtgcgcgg ctggtctcga agctggcgag accggccagc tcttcagcaa ggcgccgggt
     4621 gttctactcg aacctcagag caacggaggc tccgcgaaaa tgcccgtctt cgggcgagta
     4681 atcgcctacc tgcagcatac ccgtgtggcc ggcaagtgta ctcgggtatg tgacctcaaa
     4741 tccgaaggag ccccaggccg agtcccagtc gttggtggtg taggcgccct gcctcactac
     4801 cctgcctgcc gagttcttga gtcgccatac gccttgtgcc tcgaatgggc gtccgtaacc
     4861 gctgaccttg aacgtgtccg gtccgaccag gtaggagagg cgcggagcgg tgatcacggc
     4921 tgttttgcac tccgcgggtt ctgggtacag cggcagtcct ccgggcctga catccacgac
     4981 cacgcgggcc ggatggtcca ggctaaatac cttgaccgtt cccgccggct ttctcaggcg
     5041 caggtccacc gccagggtgt cgtcggcgcg gcgcaccacg tagtagcggg aaatccccag
     5101 accgagggcc ggaccatccg tttttagcgt ggactcaacg gcgggaaggt tgatccgcac
     5161 gatccaatca ccgttccggt atcgccatgt gtagacgggg gctacgtcgc ccggcgcatg
     5221 gtcccaccca agatccagga ccgcccgctc gtacgtgccg tgatctccga agcgcacgtc
     5281 ctggagcgtg tccgaggcag tcgcggctcc cgccggaccc atcacaggac ccatcccgat
     5341 caggagccca cccacaagga ttgcgagcag gacgattctt cccagcgctc tccggttctg
     5401 tcccgcatga tgccttcccg tctggactat cttccgggct ttcctctggg cattcatcgt
     5461 ccgctccttc acctggccct gtctgatcct caatgtaatg tgcgcggacg ccagcgtcgt
     5521 gaacaaacca gtccatctat caggtcaaaa tagatcaatt ccgctaatct caggcaggtc
     5581 gaacgatatt tggttcggtc tgtcctcgga ctcgcccgat gtcctcgccg aacgcgccat
     5641 tagcgttttc gtcacggtta ccgtgtgggt ttgtacaacg tagaatggta gggggtggct
     5701 gctcaagggt atgacgtggt cttcgtcggc ggcggtctat ccaccatgct gcttttgagc
     5761 gaattgcggt tcgtgttgcc agagcgggtg gcggttatcg agccgtcacc gccccttgaa
     5821 cgtccgtccg ttcactggag ctactggagc cgcgagcaga ccctgtatga tcggttcgcc
     5881 atcggcgtct ggcggcgggc cagggtaggg gacgcgccgc cggagccgat caccccgttc
     5941 gtcttgcggc tggtacgctc caccgacgtg ttcgctcata tcgcagcgct actggcagac
     6001 actcccatag attggctgcg aacttcggca cgcgagatct cgcgccggaa cgacgacctg
     6061 tacgagatct ccaccgacgc cggaaccgtt cacgcccgct gggtattcga cagcgtctgc
     6121 gatattgctc ccacgtttcc gtcgcagggt gagccgcgag gagtgctgag cggcaccggt
     6181 ctccgcgtta cggccgaccg gccggtgttc gacgcggcga ccgctaccct gttcgacccg
     6241 ctcgacgaac agtccttcgc gtacttgctg ccgttgagcc ccatcgaggc gctgctcgaa
     6301 tcggcctcgt tcggttcgga tgctcggaga gaagacagga gaccgctact tcagtatcta
     6361 caggcccggc acccgaaagc ccacttcacc gtaacccagg cagagtgggg agccataccg
     6421 ctcgggttcg cgccaccacg gaccgctggc ccccggcata tccttatcgg cgcgaagcgc
     6481 ggccttgtga agcccagcgc cggctacggc gtggtacgca tcgcccgtga gagcgagcac
     6541 ctgtcccgcc tctggcggga ggatccgctg ctgccgacag gttggcgcgc cccctggcgg
     6601 tggcggcttc tcgacaaagg gttcctgcta cttgccgcgc aagacccccg cctgccgatg
     6661 gcgttgctgc ggcgcgtcat gaacgctgtt cctctcgaag agtcgcttcg ttttatagac
     6721 gaggaacttc cgccgcggaa gatggcggcg tttcttccat ctgctctcct cgcggtgctt
     6781 caacgtccgc gatagttgcg cggatacgct gcgcggatac gctgcgaggg gccgtcccgt
     6841 acgagccgga gcacggcctt ggagcaccgc cggaaatggg acgttcttgg gaatgaacgt
     6901 tgggcaatta tcatcaccac aatagtccat cctgctcaag tcgtcaggct atctccgggt
     6961 tagagtccgc taagcagaca ggtaggacct tcaagtacag gagagcggac tatgatggcg
     7021 tcagcaacga ggccggactc gggggtggac agaagcgccg gctctggcgg agactgcggc
     7081 gttatagacc ttctggacgg ggtcagggtc tcttaccggc cgataagggc agaggatgcc
     7141 gccgccctgc aaaggttcca tctgcgtctc agccggcgct ccgtctacct tcgtttcttc
     7201 ggcgcgaaac cggagttgag tgacaggaag gccggttact tcgcccgcct cgacggtgta
     7261 aaccagttcg ctctcgtggc gctggacccg gaccggcccg gtgagatcgt cgctatagtt
     7321 agctactaca gggaagggag cagcgaccgg gccgagtacg ccgcggtagt cgaggatgga
     7381 tggcagggac gcggtctggg cctggaactg acccgcaggt tgatacaggc cgcgctaaag
     7441 aggggcgtcg cctgttttac cggggtcgtc ttgcccgaga acgctaggat gctcagcctg
     7501 ctccgggacc tgaacctccc ggagcatctg cggctcggag acggcctgga gtatgtggag
     7561 atagagctcg cgcccaccac caaaaagcac ccgagcatga cgccgtccct tgagtcgggc
     7621 ggcggaatcc gctgccttga gataccgtag gcggcagaca ggggagtaaa gcggtggaag
     7681 ccggtcccgt tcgggaacag agagaggaac agagagcttt cgatcccgag gagcggatag
     7741 acctcctgtt gcggcacctc gggaggcggc gggaagggct ctccgcccgc aggctagagc
     7801 aatatggacc gaacgggatc caacgccgcg agggacgcgg gcacgggtgc gccgtggtgg
     7861 tactacggtg gaagtggatg cccccaccct cgtgcccggg gacctgctgc ttctttcgga
     7921 aggagacagg ctctccgccg atgcgcgttt gatcgacggt tcggtcgaag tagatgtgtc
     7981 gcccctgacc ggcgagtccc agccggttgt acgcagcgcc acccgctccc ggtccgcatc
     8041 ctcgcctctg gagtccgacg acctcgtctt ctccggcacg ctcgtcaccg cgggcgaggc
     8101 agagtccgtc gtctacacaa ccggaatggg aacccagctc ggacgcatcg ccgccctgac
     8161 ccagcgtgtc cggggcgaga ccagcccgct ccagcggcag gtcaacgggc tctccgtgtt
     8221 gcagctcggc aacttcgcct tcggtcaccc gagccgcatc accgcccggg ttcggatggg
     8281 caagggcgag gtggtggata tagagcggga ggtagaactg ggcggaccgc tccactccaa
     8341 gggcgtgctc atcctctccg gtttcctggg cgcgcgctac gcggcggagc gtccactctc
     8401 gctctcggcc agcctcgtct tcgagcagtc ctacggcggg gtagagggcg actcggcctc
     8461 atcagccgag ctttatgccc tgctctcggc gatctccggc cttccgataa ggcagtccct
     8521 ggcggttacc ggctccgtca accagcacgg ccaagtgcag gccatcggcg gggtgaacga
     8581 gaagatcgaa ggcttcttcg acgtgtgccg ggcgcgcggc ctgaccggcg aacagggagt
     8641 ccttatactg gcctccaacg tgaagtacct gatgctccgg gaggacgtgg tgagcgctgt
     8701 ggaacaggga cgctttcacg tctacccggt ggagacggtg gacgagggca tcgaggtcct
     8761 cacgggcacg cccgccggcg aacgtgacgc ggccggcaag tacccggagg gcagcgtcaa
     8821 cggattggta gaggcgcgcc tcgccgcgct caccgagaag caagcccggt tcgggcggac
     8881 attccaggaa ggcggaccgg agtgagcgag cgggggttgg ccttgaggcg tatcctggtg
     8941 gctctggaga ccgctgccag caaggcccgc ataaactggt cctttcaagt agcgcgggga
     9001 acggtcgcca cggagatgct ggcggcggcc tctgaggcgg acctgctcgc gctcggcaag
     9061 tcgggctgga cggcggtcac ggaggggagg ctgggctcca ctgtccgggc gatagtgtcg
     9121 gcgggctccg gtccggtcct gatcctacag cccggagccc gtctcggaac gccggtgctc
     9181 gccgtcaacg atggctcttc cgccgcccgg aaggcgctgg agttcgccgc gcgtctgctg
     9241 cctgaagagg atggaggtct ctcggtcctg atcgcagcga acgacgcgga gacagcccgg
     9301 acccttcaga agcaggccgg tgaatgggcg gagaggcgga ggataaacgt gcgtttccgg
     9361 actctgctcg gtgggcgcgt cccggagctc gtccgggcgc tgggcaccga ggacgccggt
     9421 cttctcgtac tccccggacc gtttctctcc gcgaccgccg gagccttgcc tgcgatcctg
     9481 agccgtatag aacgtcccgc tctgctggtc tggtaaaggt cctgcctgtg ccggacgtga
     9541 tcctccgcgt agcgaaagct ggtcaagttg aatcatcaga atggtcacga ttagtcatgc
     9601 gcccgcctag tttccgggcg aaactccagt tgaggcgagg ccggcgatcc gcactctccc
     9661 cctccccgga tcgccggccc gccacgtaga tagtgagata gtgcgggtgc aatgcaggga
     9721 gcggtacggc gtgaaggccc tgatcctagt gccggcgcgg taaataatcg ggggaacaag
     9781 gaggtacagc tatgttgagc ccgttcagag gattcagaga cttgcagagc gagatggacc
     9841 ggctgttcgg cgagacgttt ggtcggttgc cccgaatggc ggcggagacc gaatgggccc
     9901 cggcggtcga cgtgacgacc gaggacggcg acatggtgat ccgggccgag ttgccgggag
     9961 tgaaacgcga ggacgtggat gtgactctct ccggcggcgt gctcaccatc tccggggagc
    10021 gcaaggagga gcaggaacgc aaagacgccg gttatctcgt gagggagcgg cgctacggct
    10081 cgttccggcg ggcgatgacc ctcccggagg gtaccaacga gagtaaggta tcggcgagct
    10141 tcgaggatgg ggtactggag gtgaaggtcc gggacggcgc atcggtggtc gaggaacggc
    10201 ccaggaggat agagatcgag gagtagagac tccggggact gcggtccttg gtccttgaac
    10261 ggggttcggg acgatgctgg agatgcgctg aacgcgccgc attcgagatc ggtgcgctgt
    10321 acggagagag tgcctctcaa agagaaaggg tctcgcgatg agcgacctac cggcgaggat
    10381 actcgttgca actgatgggt ccgcggacgc cgagatggcg ggccgcaggg cctccgcgat
    10441 ggcccgggcg ctcggctcgg agctgcacat ggtctacgtt gtcccggtaa cggagcctta
    10501 ctcaccgtac agcgagggag acggggaaag gccgggcacg tatgaggaca ggcgggaagc
    10561 cagagcgcta ctcgcggagc aggtcggtct ggtggaagag gcgggcggca aggtcgagaa
    10621 gtcttatatc cgggtggggg agccggacgc ggaggtagtg tcgctggccg aggagatagg
    10681 ggccaacatg atcgtcacgg gcagcagggg gctgggccgg ctgaggcgtc ctatcgggag
    10741 cacttcgagc agcatcgcgg cacacgccca ctgcccggtc ctggtcgtgc gccgggagtc
    10801 ggagagcggg gtgaaacgat gaacgaagtc ctgcggaagc tgcttctggc catcgacggc
    10861 tcggaggacg cggcgctcgc cgcgcgggca gccgtggacc tcgcggacag gaccggcgcc
    10921 gggctgcacg tcgtccacgc ctggcagccc atgtacccgt acgtgaacta cccggcctcg
    10981 gtctcgacgg acttcgtaag gatctacgag gagcgggccg ggagcctgct cggcgttcag
    11041 gtggatcaga tagaggcgga gggcggcgcg gtcgaggagt cccatctggt catgggcgct
    11101 ccggtagagg ccatcctgga gctgtgtggt cagatagagc cggacctcat cgtcgtgggc
    11161 agccggggga tggggccgat ccggcgcgtc ctaaccggca gcgtctcgga gggtgtagtg
    11221 catcacgccg gatgtccggt cctcgtggtg cggggcggcg agggagggtg gcctccatcc
    11281 cgcatcctca taggagacga cggctccggg gacgccgaac gggcggcgcg gctggcggcc
    11341 cggctcggcg cgctctacgg ggtcggcgcc gcgctggtgc gggcctacga gaaccccccg
    11401 tcgcccatcg gagggtggag cgcggaagac cggcgcaggc tcgacgagac tctggtcgag
    11461 gaagaagccg ccctcgaccg gcgggcggat gaggtcgaaa aggtcctggg cagccgtccc
    11521 gagacccgca tggtcgaggg cgacgcgacg atggccctgc tggagatcgc cggggaagag
    11581 gagaagaccc tcttcgccgt ggggagccgc gggctcggcc tgttctcacg tctgaggctc
    11641 ggcagcgtct ccaccaaggt cttgcggacg gcgcgcggcc ccgtgctggt ctgcacgcac
    11701 cactccggat aagctaactt cgtggcgatg gccggtctcc ggcgcaaatc ttcccggatg
    11761 ggtttgatca atcttgacca gacaaatggc gcgttgttgt caaggcacgg cactccggtt
    11821 cgtatatctt ggattcgtag gatagagcga tgggtcggaa ggagcggcgc catgaacacg
    11881 tccggtgcga aatccccgta tacgaacctc tcccgcagag acagggtggc gctttccctg
    11941 gaacacgagg gcaacaagag gctcaggaac ctcagtacgg tcctgtaccg gcttaccggc
    12001 ggtcggtttg ccccccacga ccgggacgtc atcctcctca cgacgcgcgg ccggaagtcc
    12061 ggcagggagc atactgtcct cctccagtcc ttcccggacg gcgatgacat gatcctcgtc
    12121 gccgccaact ccggccggtc ctcccacccc gactggtttt acaacttgaa agccgagccg
    12181 gcggcgcggg tcgagatcga ggaccgcacc ctgatggtcc gtgccgggca ggtgccggag
    12241 gatgaggcgg aagccctctg gccgggcatc ctgcgccatg cgcccacgta cgcccgttac
    12301 cggagggcta cagaccgcgc cataccgctc gtgcggctcg tccccatcgg gtcggacgaa
    12361 ggatgaaagg gaggcggacg tgctctctgc cggaatcatc aggggcataa ttaccgtgac
    12421 gatcctgggc aacggtatcg ctcatgcgac cgcgctcgtg gcactcattc gccacagtct
    12481 gggtggcacc tccgcttctc gggtcgcggt tcgatcgtga ggttctccat ggtgtccgcc
    12541 ctttcatgta agcgctcagc atcgcggttc gccgcctccc ggcatcggtg tggcgcttca
    12601 aagaattatg tccgagaagt cggtcggaga acattcgggg ttagagtatg aagaagagaa
    12661 ggagttaccg ggttgtcccg ttcacgatga acaggcgcat ggtggccgct tccgcttccg
    12721 taggccgcga gcgaaacaac atccatgcga tgatcgaggt cgatgtcacc gagccgcggc
    12781 gcctcatgcg tgagcatcgc gagcggaccg gagagaggct ctccctaacc gcgttcgtag
    12841 taacatgcct ggctcgggcc atcgccgaga acccgaacct caactcgttc cgcaagggca
    12901 ggaagctcat actgctcgac gatgtcacgg taagcgtgct tgtggagcgg gagatagccg
    12961 gagagaaggt gccggagcct ttcggcatta gagcggcgca gaccaagacg taccggcgga
    13021 tccacgacga gatccgcgcg acgcagcgac acggcgaaga cgggctcggg ggtctctccg
    13081 gaataagctg ggtgcggttc attccaggtt tccttctgcg cgccttcgtc cgtgtcgcat
    13141 cccgcaacat ccacatgatg gaccgttttg gcgcggtcgg cgtaacggcg gtcggcatgt
    13201 tcggtggcaa tgaagcgctt tggctcattc cccttagcgg ggcaaccgtc gcggtcaccg
    13261 tgggaggcat cgtggagaga ccgtccgcca gcggtggccg gatcgaaaca cgtgagcacc
    13321 tctgcctgac ggtgacgttc gaccacgaca tcgtggacgg cgtgccggcg gcgcgtttcc
    13381 taaagagatt ctccgagttg ctcatgatca gcgaaccgct tggtgacacg atagtaactg
    13441 cagcggtgga atgaaggagc gcaccatgaa cacgctgccg accaaacgta acctcacact
    13501 ggcctaccgt ctctcactcg tggttgccgc tctcatggca atcgcgtcta tcgcgggcat
    13561 cttgctcggc tccgccggac tctacggggc tgacccgaaa ctcgccctcg gcgtgactga
    13621 agccgaagcc gggctgctcc tgcccggtct gctgggtcag gacctgttca acctcgtcgt
    13681 gggggtgccg ctgctgctcg gctcgatgtg gctcgcccgg cgtggatctc tcatcggcct
    13741 cctcttgtgg ccgggcatgc ttttctacgc gctctactgg tacgtgctct acgccgtcgg
    13801 cgcgccgttc agcgtgctgt tcctgctgta cgtccccctc gtcaccctta gcgcgtacgc
    13861 gacaatcgcc ctggtatcca gcatcgacgg cgagagcgtg cggcggctca cggaggccgt
    13921 gcccgcaagg accatcggcg gcatcctcgt cgtcctcgcc ctccttacgc tggcccagga
    13981 cgcgaccggc gccttcgtaa ccgcgctggc cgggaacgcg cccgtggacc cggcggcgcg
    14041 ccatgtctgg atctccgacc tcgcgctgca ggctccggcg gtgctcgccg tcggagcgct
    14101 gctgtggcgc cgccatccac tgggctacgt gaccgctgcg gggctgctgt tccagtacgg
    14161 ccttactccc atcgggctcg tcgccggcat ggcgctccag gccgccctga gcggctcgcc
    14221 gctcgatgcc gggaccagcg gcgtgctcct cgtcttcagc ggggtctgct tcgtatccct
    14281 cgtgttcttc gtccgcgccg cggcccggcg ccgtctgccg tcgcccggta cggagactac
    14341 ggggacgtcc acggggatgt ccaccgggta ctccggggcg ggggatgagc gcggagtccc
    14401 ggatgtcgcg gatgatcgga tggaggacgt acctgcggtg aagcggagtt ggagaggcgg
    14461 gagagaagga tgagagctgc aaccggaact tcgaggtcct ccgggggcgc cgctaccggc
    14521 ccggacctgt cgtggagggg tctctaccgg gcgggagccc tttcggcggt cctctacgtc
    14581 gtcctgatcg tggtgccggt cgtgctcctc ttcgcggcgc cgcagcctcc gctctcgggc
    14641 ggggacgaga tactccggta catcgccgag aacaaggcag tctacgtgat cgaactcgtg
    14701 tctttcgtgg ggttgagcct gccagccatc gtggtctttc tggccctcta cgttgcgcta
    14761 aggcacctgg acagaagcta cgccgcgctg ggcgccgtgg tcggcatcgc ctcggagatc
    14821 gtcgccctgg cctacaacag cagccctcct tcgctcaacg gcggtctgct gtacctgagc
    14881 gatcagtacg cggcggccgc caccgacgca caacgcgccg ccctcgctac cgccgccgaa
    14941 gggcttgttg cggtgagcaa cgcggtgaac gccgccggca tcctgaccgc gatcggcatc
    15001 ctgatcatct cgctggttat gctgaagggc gccttcccca ggggcgtcgc gtatctgggc
    15061 atcgcggccg gtgcggtagg catcgtaagc gaggctctac gggacatgat cggcgctggc
    15121 tacttcgtct acggactcct gcttccgagc tggttcatcg cggtcggatg gacgctcttc
    15181 aggctggccg gaggcggatc gatccggatg aagcgcgctg gttgagcggg ggatgagaga
    15241 acgagaagga gaggatcatt gtgtggtggg cagacttgct gtggggatta tggaacgggc
    15301 ttacggcctg gatcgtcttt atcgtacacg tattcggcgg ctgggagcag ttcccgttct
    15361 acaacgctgc gcgcggcggg aactggtacg acttcggttt cttactcggc gccgggtctc
    15421 cgcttctcgg agcttccagg gggaggtcca gggcgactta gtgacggtaa ggtccttatc
    15481 ctggagtccg cttctcaaac ggagtaatgc gttctatccg gaaggcgttg gcttgggaag
    15541 catatggacg gtaagaggtt gtctcatgcc gcaatcagac ggagagcggg cacgttttag
    15601 tcaatgacga tcaatgattt gggcaatgtt gatcaagtca gggccggtac gggctgagta
    15661 gagttggacc atgttcgcag cagtcgcctt tgccgatgta gagggaggtc caagatgaac
    15721 gccaactcag ccgccggacc gcagcccgga gcgtacagag cgctcgtgct ggcgaccctg
    15781 gccttcgccc tgtgtttctc ggtgtggggc ctcgtggcgc cgctggcgcc gcagtttcag
    15841 gagctataca agttgtccag cactcagatc agcgtcgtca tcgccacgcc ggtgctcctg
    15901 gggtcgctgc ttcgcatccc gataggcttg atcaccgacc gcttcggcgg ccgggccgta
    15961 ttcgcggtat tgatgctggt cctgatagtg ccggtgatgt tcatcgggct tctgggcggg
    16021 tctttcgggg gccttatctt ctggggtttc ttcctggggc ttgccggtag ctcgttcgcc
    16081 gtcggcatcc cgttcgtctc gaaatggttc ccgccgcaca tgcagggcct cgcgctcggg
    16141 atctacggca tgggcaacat cgggaccgcg atagccgctt acaccgcccc ttctatcgcc
    16201 gcctcctatg gctggcagtg ggcgttctgg gtgttcatca tcccgctcgt agcgatggcg
    16261 gcggtcttct gggtcatggg ccgcgacgcc ccgagcacgg gaccgaggcc gtcggtcacg
    16321 gcggggatag cgctcttccg cgaggaggtc atgccctggg tcctgagcct gttctacttc
    16381 cttaccttcg gcgggttcgt ggccctgggc atctacctgc ccaagctgct cgtcgacctg
    16441 ttcggcttca ccccgtcggg cgcggggttg agggcggcgg gtttcgtggt gctggcgacg
    16501 ctctccaggc cggttggcgg atggctcgcg gaccgtatcg gcggctccag gacgctcatg
    16561 gtcatcttcg ccgtcctgcc ctttatggcc ctgatcctga gcttccagcc cggcatagtc
    16621 ttgtttacct tcgcggccct ctccagcgcg gtgctactgg gcctcggcaa cggcgcggtc
    16681 ttcaagctgg tcgccgagta ctacccgacc aagacgggtg cagttaccgg cgtcgtcggc
    16741 gcggccggtg ggctcggggg cttcttcccg ccgctggtcc tgggaatcgt gcgggacgcc
    16801 accggctcct acgccatcgg gttcgttctg ctggctctgt tcgcggtcgg ctgcttgctg
    16861 gtgaacgcgc tgttcgtccg gcggcatcca tccgggacgg gcgtcgcgca gatgagagga
    16921 gcctagtagc gtggctcgcg cgacgaatcc tctgctcaga gacctgcaat acttccgccg
    16981 gggagagaag cgcgccggcg gctggagcga actgaacgcc cgcgacaggg agtgggagta
    17041 cgcctaccgc cagaggtggc agcacgacaa gatcgtgcgc tccacccacg gcgtgaactg
    17101 caccggctcg tgctcctgga aggtgtacgt caaggacggc atcatcacct gggagaccca
    17161 gcagaccgac tacccctcga acggccccga ctcacccgag tacgagcccc gcggctgccc
    17221 tagaggggcg tccttctcct ggtacacata ctccccgatc cgcataaagt acccctacgt
    17281 ccgcggcgtc ctcatgcaga tgtaccgcga ggcgaaagcg caagccggag acccggtcga
    17341 agcgtgggcc tctatagtcg aggacccgga gaaagcgcgg gcgtacaagg cgcagcgggg
    17401 caagggcggg ctggtgcgcg cc
//
LOCUS       DASSMB010000073        11633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_396820, whole genome shotgun sequence.
ACCESSION   DASSMB010000073 DASSMB010000000
VERSION     DASSMB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 11633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11633
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396820"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            507..1592
                     /gene="rfbB"
                     /locus_tag="VFJ72_04880"
     CDS             507..1592
                     /gene="rfbB"
                     /locus_tag="VFJ72_04880"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET7478833.1"
                     /translation="MEAEKQTILVTGGAGFIGGNFVLGAVGSGSFRIVNLDALTYAGN
                     LDTLASVAENPDHVFVHGRIGDRELVDRLLAEHRPSAIVNFAAESHVDRSIDGPGAFI
                     QTNVVETHDLLEAALVYWNTLEGEERDRFRFLHVSTDEVYGTLGPTGYFTEESPFRPN
                     SPYAASKAASDHLVRAYHHTYGLPTLTTNCSNNYGPYHFPEKLIPLMILTALRGDPLP
                     VYGDGQQVRDWLYVEDHCRAIATVLAMGAVGETYNVGGHNERANLDVVKTICALLDEL
                     APESPHAPHEQLVTFVTDRPGHDRRYAIDAGKIERDLGWTPEETLESGMRKTVRWYLE
                     NRGWCEGILSGKYRGERLGLGSTWSRT"
     gene            1589..2470
                     /gene="rfbA"
                     /locus_tag="VFJ72_04885"
     CDS             1589..2470
                     /gene="rfbA"
                     /locus_tag="VFJ72_04885"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET7478834.1"
                     /translation="MKGIVLAGGSGTRLYPLTRAVSKQLLPVYDKPMIYYPVTTLMLA
                     GIRDILVISTPHDLPRFEDLLGDGSKWGVNFSYAVQPEPEGLAQAFIIGREFVGRDRV
                     SLVLGDNIYYGHGFPELLARAASREDGATVFGYYVRDPERYGVVEFDQDGTVLSIEEK
                     PEKPRSHYAVTGLYFYDNRVLDVAENLEPSPRGELEITDVNVDYLERGGLQVELMGRG
                     MAWLDAGTHESLLQSANFIETLEERQGLKIGCPEEIAYRKGYITAEELEELAYPMRKN
                     NYGQYLLELLKSEEFVL"
     gene            2467..3027
                     /gene="rfbC"
                     /locus_tag="VFJ72_04890"
     CDS             2467..3027
                     /gene="rfbC"
                     /locus_tag="VFJ72_04890"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET7478835.1"
                     /translation="MNVIETELPGALIIEPKVFGDARGFFMETYNDARYAEAGLPERF
                     VQDNLSYSARGVLRGLHFQNPRPQGKLVSVLQGEVFDVAVDIRVGSPTFGKWTGVTLS
                     AENKRQFYVPPDFGHGFVVTSETALFSYKCTDYYAPSYEGSILWNDPEIGIEWPIEEP
                     ALSEKDRSALPLKEISEDVLPRYGTG"
     gene            3031..3429
                     /locus_tag="VFJ72_04895"
     CDS             3031..3429
                     /locus_tag="VFJ72_04895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7478836.1"
                     /translation="MGGENTFDILEGRRYMRLTTYRRSGEGVPTPVWFARVGGRLVVV
                     TDSESGKAKRIRNNPRVMLAPSDFRGRPRGGSVEAMARIMDGREGESADRALLQKYGW
                     QYRIFNQVIRWQGASDRRVFLEISPVSDER"
     gene            complement(3426..4289)
                     /locus_tag="VFJ72_04900"
     CDS             complement(3426..4289)
                     /locus_tag="VFJ72_04900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7478837.1"
                     /translation="MKFSVVIVNYASWPLTLRCVESLIATNYESFEVVVVDNDQSLPP
                     ELPRATRLIRNSDNVGFARACNQGIAASTGELVALINPDTTVERDFFERVEQFFEENP
                     RAGVAGPRILDSAGNLQLSARREVTLITGLLGRTSLLTRLFPKSSLVKSQFPAVTSTA
                     VPAKVDWVSGACMAVRRKTLEEIGGLDERFFMYFEDADLCRRVREADWFVYYLPQIEV
                     VHQTGGSSRSRPKAIWLLHKSAFLYHRKHGAHGPLNLYSAAVLAGLAARALAKFATSF
                     IASLPGKKSSR"
     gene            complement(4286..5296)
                     /locus_tag="VFJ72_04905"
     CDS             complement(4286..5296)
                     /locus_tag="VFJ72_04905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7478838.1"
                     /translation="MSRVSVVIPNWNGAKFLGVCLSSLHSQSFEDFETILVDNGSTDG
                     SVEFVKSNFPEVRVYALEDNHGFSVAANAGIEASGTEYVALLNNDTETVPGWLEALVR
                     SAEAHPEAGLFASKLVDFHNRRFLDGAGDAIRLSGLPYRLGHGERDRGQFDEISYVFG
                     ACAAAAMYRRKMLDEIGFFDEDFVSYCEDGDVSFRAQLADYHCLYVPDAVVYHMGSAS
                     TGGKRSATATRLGTRNSLSLLVKNLPLSVVPHVLPFFVLGQLARLLTAAATGTLRPHL
                     KGLAEAWDQLPLTLKKRREIQSRKKVSDARVRRLLRGSSLAATRSIACRLRDRVQTRL
                     GS"
     gene            complement(5301..6644)
                     /locus_tag="VFJ72_04910"
     CDS             complement(5301..6644)
                     /locus_tag="VFJ72_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7478839.1"
                     /translation="MHAISLEGISKKYRIYPRQRDRLREILSFGKVKRSSDFWALKDV
                     TLKIEPGVSLGLLGRNGAGKSTLLQIVSGVLQPTTGVARTNGRISALLQLGAGFNQEF
                     TGRENALMNGLLLGIDRKEMIHRFDEIEAFADIGEFMDQPVKNYSSGMRARLGFAVAV
                     NVEPDILLVDETLSVGDGVFRHMGIQKMRELRDSGATIIFVSHSTTMVKNFCTDAALL
                     QKGRLIAHGDVSETIDYYQALLSNAAAQKDTQLHLSNQPLELETENEDDADTPGFKED
                     PTLQNRGPRLRHGTGEARIQSVEVLNAQGAPSDFIDPHSPMTVRVHVKYKADVNDSVL
                     SITLRNKTGLDVYSTNTNLEKAPIKARHAGDRIIVDFSIQPLLRHGPYSVNAAVSHGQ
                     NKEAHMDWIDVATAFDIGRPVSRGAFAGLVHLPTQVTVFEPEHAHDSGRGPEQSA"
     gene            complement(6644..7390)
                     /locus_tag="VFJ72_04915"
     CDS             complement(6644..7390)
                     /locus_tag="VFJ72_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478840.1"
                     /translation="MQRDLTRSYRGSFLGLVWLVLGPLLMVILYTLVFSKVLGLRFHQ
                     TDSVTNYGLYVYCGLLPFQAFSDALNKSVTSIRTNSTLVKRVVFPLEILPMTTAATAF
                     VGQFFGFGALMVLQIVLEHHLQWTLLLIPIIAVPQLLFMLGLSYLISVAGAYMPDLKD
                     SLRAFLRGFMFATPIIWPASRVPEDSPMRPFIDFNPLAFISSAYRDLVLSGDLPSLGG
                     LLLFTLFAATVCLVGVTLFARIKRQFADLI"
     gene            complement(7842..8948)
                     /locus_tag="VFJ72_04920"
     CDS             complement(7842..8948)
                     /locus_tag="VFJ72_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HET7478841.1"
                     /translation="MYSEDLRWKIDRRDFLRLGGAGLAGAVLLGTAGKVLAQTGSSLV
                     LEFEAAATEYNVPKELLLAMGYVNTRWEMPPPEASNYEAGDIEGRGYYGIMQLMQNPS
                     RDTLGRAASLTGLSEEELKTNRAANVRGGAAVLADIQGPNKPSDLNGWQETVAGYGDT
                     DLYAIAVYETLKSGASTTTSSGESLELAAQDVEVPQVFTAMAATDYPPALSHPAYSGN
                     YTNSNREASYNIDRIIIHVTQGSYVGAINWFQDPSANVSAHYVVRSKDGRVAQCVRNA
                     DIAWHAGNWTYNTHSIGIEHEGYVSQRKWFTRAMYRHSARLAAWCCKRHRIPVDRQHI
                     IGHYQVPGSDHTDPGPYWNWTNYMRLVHYYRRRM"
     gene            complement(8979..10460)
                     /locus_tag="VFJ72_04925"
     CDS             complement(8979..10460)
                     /locus_tag="VFJ72_04925"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7478842.1"
                     /translation="MGLVGPSSHATAQQASISDEFGSAANESNVPKELLLAMGYVNSH
                     WEMPPPTASDYDKNDPEGRGTYGVMALVQNPSRDTLGKAASLTGLSQDQIKSERDANI
                     RGGAAVLADIQGSQKPSDLSGWYDAVSKYGGSGQYADQVYKVLKSGASATISTGEKVD
                     LAPQNVDVPQLFTAQATGDYSGSTWYGNGGSNYTPSSREASYDINTIVIHVTQGSWSS
                     AINWFASPSNTGSSAHYTVRSSDGFIGQSVHQDDIAWHAGWWPTNTHSIGIEHEGFIS
                     DPSWFTDAMYRSSARLSAYLAKKYNIPIDRTHIIGHNEVPGCSGVGGGGDCHTDPGPY
                     WDWTKYMNLVKSYAGTSTATTSPNVQIIDNSSAQRFRASSKWTNSSFSSQRYGNNYRA
                     LLKPLSVYDPAQYKVKIPSRGSYTVYGWWPSNSGYTGRATYKIRTVNGTVNKTVSQRT
                     NGGKWVSLGTYTMAAGDSWSVGISSKSSSTGYIIADAIKVVKR"
     gene            10818..>11633
                     /locus_tag="VFJ72_04930"
     CDS             10818..>11633
                     /locus_tag="VFJ72_04930"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7478843.1"
                     /translation="MTRNSGVPGYKPSTLTRIKGVLRRVVGRGRRLAISKRIPKDGAE
                     TRVRPENVVWIFGSGRTGSTWLAAMMGEIEGQTVWREPLVGALFGNLYYVRAEHRIDK
                     RGEHFILGGAYKDTWMGPMREMVLGGAAARFPELTRGGYLVVKEPNGSIGAPLLMEAM
                     PESRMVLLIRDPRDVVASSMDARREGSWLHERRNTDSRKRESLPDKNPNVYVKTRAKT
                     YLQHAGNAKRAYDAHRGYKTVVRYEDLRADTLATMKRLYAELGIPVDEEGLIRA"
BASE COUNT         2288 a         3381 c         3642 g         2322 t
ORIGIN      
        1 cccgtcgggt tcatcccctc gaacttcagg tagacgcggg cgccgacgcg ctcggagagc
       61 ttcgtggctt ctatgagggg cgtctcgccc tcgccgagcg tcgcgatggc atcgtccggg
      121 atctccggca gccattcacg atagttcctt accactcctt gtctcaaagc tcgcgcctga
      181 acgctcaaag agatatcgcc tttcctgtaa tatccggccg gctcgtcgcg gaccggagga
      241 tcaacaaccc gtaccggagg cgcaaccccc ggcatcggcg gctcgcgtga atgtgagaga
      301 gatgtagagg ttcggatcca cagcgtacga aaagtatcac gccccgcgcc gcacttgcaa
      361 caatgccgca tggcgaccgc aatagcgacc gcaatggcga ccgcaaccct cgtctagtaa
      421 attacgggct tgagccgtca gctattagcc aggataagcg ctgatggctg aagaccgata
      481 gctggtagct acagcgaagg agcgcgttgg aagcagagaa gcagaccatc ctcgttactg
      541 gcggcgcggg gtttatcggg gggaacttcg tgctcggcgc ggtgggtagc ggcagtttcc
      601 ggatcgtgaa cctggacgcc ctgacctatg ccgggaacct ggacaccctc gcctccgtcg
      661 ctgagaaccc ggaccacgtc ttcgtccacg gtaggattgg agaccgggag ctggtggacc
      721 gcctgctcgc cgaacaccgt ccctctgcca tagtgaactt cgcggccgag agtcatgtgg
      781 accgctccat agatggtccc ggcgccttta ttcagaccaa cgtcgtcgag acgcacgacc
      841 tgctggaggc ggctctcgtg tactggaaca cgctggaggg cgaggagcgt gatcggttcc
      901 gtttcctgca cgtctcaacg gacgaggtct atggcacgct cggcccgacg gggtacttca
      961 cggaggagag cccgttccgg ccgaactctc cttatgcggc gtcgaaggcg gcctcggacc
     1021 atctggtgcg ggcgtaccat cacacctatg ggttgccgac gctcacgacc aactgctcca
     1081 ataactacgg gccgtaccac ttcccggaga agctgatacc gctaatgatc ctgaccgctc
     1141 tgcgagggga tccactcccg gtctacggcg acggacagca ggtgcgcgat tggctctacg
     1201 tcgaggatca ctgccgggcc atcgccacgg tgctggcgat gggcgcggtc ggcgagacgt
     1261 acaacgtcgg cggccacaac gagcgggcga atctggacgt ggtcaagacc atctgcgcgc
     1321 tgctggacga gctggcgccg gaatctcctc acgcgccgca cgagcagcta gttaccttcg
     1381 tcaccgatag gcccggtcac gaccggcgct acgccataga cgccggaaag atcgaacgcg
     1441 atctcggctg gacgccggag gagacgctgg agagcggcat gcggaagacc gtccgctggt
     1501 acctggaaaa ccggggctgg tgcgagggta tcctctctgg aaagtaccgg ggcgaacggc
     1561 tggggctcgg gagcacgtgg agccggacgt gaagggcatc gtgctggcgg gcggttccgg
     1621 gacgcggctc tatcctctga cacgcgcggt aagcaagcag ctacttcccg tctacgacaa
     1681 gccgatgatc tactacccgg tcacgacact tatgcttgcg ggcatccggg atatcctcgt
     1741 gatctcgact ccgcacgacc tgccacgttt cgaggacctc ctcggggacg gctcgaagtg
     1801 gggtgtgaat ttcagctacg cagtgcagcc cgagccggag ggactggcgc aggccttcat
     1861 tatcggacgg gagttcgtgg ggagggaccg ggtctcgctc gtcctcgggg acaacatcta
     1921 ctacgggcac ggcttcccgg agcttctggc gcgggccgcg agccgcgagg atggggcgac
     1981 ggtattcggc tactacgtgc gcgaccctga gcgctatggc gtggtcgagt tcgaccagga
     2041 tgggactgtc ctgagcatcg aggagaagcc ggagaagccg cgttctcact acgctgtgac
     2101 gggtctttac ttctacgaca accgggtgct ggacgtggcg gaaaacctgg agccctcacc
     2161 gaggggagag ctggagatca cggacgtcaa cgtggactac ctggagcgcg gcggccttca
     2221 ggtcgagttg atgggccggg ggatggcgtg gctcgacgcg gggacccacg agtcgttgtt
     2281 gcagtccgcg aacttcatcg agacattaga ggagcggcag ggcttgaaga tcgggtgtcc
     2341 cgaggagatc gcataccgca agggctacat taccgccgag gagctggaag agctagccta
     2401 cccgatgagg aagaacaact acggccagta cctgctggag cttctgaaga gcgaggagtt
     2461 cgtgctgtga acgtcatcga gacggagctg ccgggcgcac tcatcatcga gccgaaggtc
     2521 ttcggcgatg cgcgcggctt cttcatggag acttataacg acgcgcgtta cgcggaggcc
     2581 gggctccccg aacgcttcgt ccaggacaac ctctcttact ccgcgcgggg cgtgctgcgc
     2641 ggcctccact tccagaaccc ccggccccag ggaaagctgg tctccgtatt gcagggcgag
     2701 gtcttcgacg tggcggtgga catccgggtc ggctccccga ccttcggcaa gtggacgggc
     2761 gttacgctct ccgccgagaa caagcgccag ttctatgtcc cgccggactt cggacacggc
     2821 tttgtcgtta ccagcgagac cgccctgttc tcctacaagt gcacggatta ttacgcgcct
     2881 tcctacgaag gctccatcct ctggaacgac ccggagatcg ggatagagtg gcctatcgaa
     2941 gaacctgcgc tctccgaaaa agaccgctcc gctctcccgc tgaaggagat ctccgaggac
     3001 gtactgcctc gatacgggac gggttgacgg atgggcggag aaaatacctt cgacattctc
     3061 gaaggccgcc gttacatgcg cctcactacc taccgccgga gcggagaggg cgtcccgaca
     3121 cccgtgtggt tcgcgcgagt gggaggcagg ctcgtcgtgg tgaccgactc cgaatcaggc
     3181 aaggctaaac gtatccgtaa caaccctcgc gtcatgcttg ccccgagcga cttccgggga
     3241 cgcccccgcg gtgggagcgt ggaggcgatg gcccgcatca tggacgggag ggagggcgag
     3301 tccgcggacc gggcgctcct tcagaagtac ggctggcagt atcggatatt caaccaggtg
     3361 atccggtggc agggcgcgtc agaccggcgg gtgttcctgg agataagccc ggtctcggac
     3421 gaacgctagc gactcgattt tttgccgggg aggctcgcga tgaacgaggt cgcgaacttc
     3481 gcaagagccc tcgctgcgag cccggcgagt acggccgcgc tgtagaggtt cagggggccg
     3541 tgggctccgt gcttgcggtg gtagaggaag gcgctcttgt gcaggagcca gatcgccttc
     3601 ggcctgctgc ggctgctgcc gccggtctgg tggacgacct ctatctgcgg caggtagtag
     3661 acgaaccagt cggcttcgcg aaccctccgg cagaggtcgg cgtcctcgaa gtacataaag
     3721 aaccgttcgt ccaggccgcc gatctcctcc agagtcttgc gccggaccgc catgcaggcc
     3781 ccggagaccc agtcgacttt cgccgggacg gcggtgctcg tgacggccgg gaactggctc
     3841 ttcacgagcg agctcttcgg aaagagccgg gtcaacagag acgtgcggcc caggagaccg
     3901 gtaatgagag ttacctccct gcgggccgag agctggaggt tgccggccga gtccaggatt
     3961 cgcggacccg ccaccccggc tcgcgggttc tcctcgaaga actgttccac acgctcgaag
     4021 aaatctctct ctaccgtcgt gtcggggttt atgagcgcga caagctcgcc ggtggaggcg
     4081 gcgatgccct ggttgcaggc acgggcgaac cccacgttat cggagttccg gatcaggcgc
     4141 gtggcacgcg gaagctctgg gggcagcgac tggtcattgt ccacgacaac gacctcgaag
     4201 ctctcgtaat tcgttgcaat gagggactcg acgcacctca aggtaagcgg ccaagaggcg
     4261 tagttgacga tgacgaccga gaatttcacg aaccgagacg tgtctggacg cgatcccgta
     4321 gcctgcatgc gatgctcctc gtcgccgcga gggaagatcc ccggagcagc cgccggacac
     4381 gggcatcgga gaccttcttg cggctttgta tctcacgccg cttcttgagc gtgagcggta
     4441 gctggtccca tgcctccgcc agccccttca ggtgtggacg caacgtaccc gtcgcggcgg
     4501 cggttagtag acgcgcgagt tgtcccagga cgaagaacgg cagtacgtgc ggcactacag
     4561 agagcggcag gttcttcacc aggaggctca gggagttgcg ggtgccgagc cgggtcgccg
     4621 ttgcgctgcg cttgcctccg gtcgaggcgc tgcccatatg gtagacgacg gcgtcgggga
     4681 cgtagaggca atgatagtcc gcgagttgcg cccggaagct gacatcgcca tcctcgcaat
     4741 atgagacaaa gtcctcatcg aaaaagccga tctcatccag catcttccgt cgatacatcg
     4801 ccgccgccgc gcacgccccg aagacgtagc tgatctcatc gaactgtccc cggtcacgct
     4861 cgccgtgacc gagccggtag gggagaccgc taaggcgtat agcatctccc gctccatcaa
     4921 gaaaacgccg gttgtgaaag tccaccagct tgctcgcgaa gagacccgcc tccggatgag
     4981 cctccgccga ccgcaccagc gcctccagcc agccagggac cgtctctgtg tcgttgttca
     5041 gcagcgcgac gtactccgtc ccggaagcct ctatccccgc gttcgccgcc acgctgaagc
     5101 catggttgtc ttcgagagcg tagacgcgga cttcggggaa gttacttttt acgaactcca
     5161 ccgaaccatc cgtggagccg ttgtctacca gtatggtttc aaagtcctcg aaagattggc
     5221 tgtggagcga gctcaggcag actccgagga acttcgcgcc gttccagttt ggaatgacaa
     5281 ccgagacgcg ggacacgcct tcaggcggat tgctcggggc cgcgcccgga gtcgtgggcg
     5341 tgctctggct cgaaaactgt cacctgcgta ggcaggtgaa ccaggcccgc gaacgctccc
     5401 cggctgaccg gacgaccgat atcgaaggcg gtggctacgt cgatccagtc catatgcgcc
     5461 tctttgttct ggccgtggga gacggcggcg ttcacagagt agggaccgtg acggagcaag
     5521 ggttgaatgc tgaagtccac gatgatgcga tccccggcat ggcgcgcctt gatcggcgcc
     5581 ttctccaggt tggtgtttgt ggagtagacg tcgagaccgg tcttgttacg gagcgttatg
     5641 cttagaacac tgtcgtttac gtctgctttg tacttcacgt ggacccgcac ggtcatgggc
     5701 gagtgcgggt cgatgaagtc cgacggggca ccctgagcgt tcaggacttc gacgctctgg
     5761 atcctggcct cccccgtacc gtgacgaagg cgaggacctc ggttctgcag agtggggtcc
     5821 tccttgaagc ccggcgtatc ggcatcgtcc tcgttttctg tctctagctc cagaggctga
     5881 ttggatagat ggagctgggt gtccttctgc gcggcggcgt tcgagagcag ggcctgatag
     5941 tagtctatgg tctcgctgac gtcgccgtgt gctatgaggc gccccttctg cagcagcgcg
     6001 gcgtcggtgc agaagttctt taccatcgtg gtgctgtggg agacgaagat gatagtcgct
     6061 ccggaatctc tcagttcgcg catcttctgg atgcccatgt gccggaagac gccatcgccg
     6121 acggaaagag tctcgtcgac caggaggatg tccggctcca cgttgaccgc tacagcgaag
     6181 cccagcctgg ctcgcatacc gcttgagtag ttcttgaccg gctggtccat gaactccccg
     6241 atatccgcga aagcttctat ctcgtcgaag cggtgaatca tctccttgcg gtctatgccg
     6301 agaagcagcc cgttcatcag ggcgttctcc cggcccgtga actcctggtt gaaacccgcg
     6361 ccgagttgga ggagggcgga gatcctgccg ttcgtccggg cgacgccggt agtcggctgc
     6421 aggacgccgg aaacgatctg gagcagggtg ctcttacccg caccgttgcg accgaggaga
     6481 ccgaggctta cccccggttc tatcttcagc gtgacatcct ttagcgccca gaagtcactg
     6541 ctgcgcttga ccttcccgaa gctcaggatc tcgcgcagcc ggtcgcgctg gcgtgggtag
     6601 atacggtact tcttggatat tccttcaagg gagatggcgt gcattagatc aggtccgcga
     6661 actggcgttt tatcctggcg aatagcgtga cccccacgag acacaccgtt gcggcgaaga
     6721 gcgtgaacaa tagaagtcct cccagggatg gtaaatctcc gctcaggacc aggtccctat
     6781 aggcactgga gatgaaggca agtgggttga agtctatgaa cggtctcata ggagaatcct
     6841 cggggactct actcgccggc cagataatag gggtagcgaa catgaatcct cgcaggaaag
     6901 cccgtaggga gtctttaagg tcgggcatat aagctccggc tacagatata aggtagctca
     6961 agcccagcat gaagagcaac tgtggcactg caattatcgg gatcagaagc agcgtccatt
     7021 gcaggtgatg ctccagaaca atttggagta ccattagcgc cccgaaaccg aagaactggc
     7081 ccacgaaagc agtcgccgct gttgtcatcg gcaagatctc cagggggaat accacccgtt
     7141 ttaccagggt tgagttggtc ctgatgctcg tcacggactt gttcaacgcg tcggagaacg
     7201 cctggaaggg gagaagcccg cagtatacat agagaccgta gttcgtgacg ctgtcggtct
     7261 ggtgaaaccg aagtcccaga accttagaga agaccagagt gtagaggatg accatgagta
     7321 gagggccgag gactagccag acgagtccca ggaaagagcc ccgataacta cgggtgagat
     7381 cgcgctgcac aaagtacccg accagccacc gccggtcgta gagcgtgcct aaagtgccca
     7441 gtaatgcccc acgaggattc tttgcgctag acaagtttgt agacaatatc gtccttcatc
     7501 tcgaccgtta gtctaatcta agtcctggac aatgcagcaa atctgcgcaa gtttaccgta
     7561 accgacagga gcccgccaga ttgatccggc ttactgggcc ggctaacgga ggacccttat
     7621 tgattatatc ccgcctctag tagagtagcg ggaatcgtgc ctataacggt agctttagat
     7681 aggtttcatc ctgttcccac tctgcgccct tgaatacatc cgcgtcggag gtgccgtcgg
     7741 cgcttgcaac cctggagagg ggtgctgtta gaagcgtgta aaaccgccgg atgttgctcg
     7801 attgagagag cctgattttg agcttcctta cttgagtttc cttacatgcg cctgcggtag
     7861 tagtgtacga gtcgcatata gttcgtccaa ttccagtatg gtccgggatc tgtgtggtcc
     7921 gagccgggga cctgatagtg gccgatgatg tgctggcggt ccaccgggat gcggtgtctc
     7981 ttgcagcacc atgccgcaag cctcgccgag tgacggtaca tggcgcgcgt gaaccatttg
     8041 cgctggctca catagccctc gtgctcgatg ccgatgctgt gggtgttgta ggtccagttg
     8101 cccgcgtgcc aggctatatc agcgttacgc acgcactggg caacccgccc atcttttgat
     8161 ctcaccacgt agtgggccga gacgttggcg ctcgggtcct ggaaccagtt gattgcgccc
     8221 acataagagc cctgggtgac gtggatgata atcctgtcta tgttgtacga cgcttcgcgg
     8281 ttcgagttgg tgtagttgcc ggagtacgcc ggatgtgaca gggccggcgg gtagtcggtg
     8341 gccgccatgg ctgtgaagac ctgcggcacc tcgacgtcct gtgcagccag ttccaggctc
     8401 tcaccagacg aggtcgtggt tgaagcccca ctctttagcg tctcgtatac agctatagcg
     8461 tacagatcgg tgtcaccata gcctgctacc gtctcctgcc agccgtttag gtcggaaggc
     8521 ttgttcggac cctgtatgtc ggccaagacc gcggcgccgc cacgaacatt agcagcccga
     8581 ttggttttca gttcctcttc cgagagccct gtaagactcg cggcgcggcc cagagtgtct
     8641 ctggaaggat tctgcataag ctgcatgatc ccatagtagc cgcgaccttc gatgtcgccc
     8701 gcttcgtagt tgcttgcttc tggagggggc atctcccaac gggtgttgac atagcccatc
     8761 gcgaggagca actctttcgg tacgttgtac tcggtcgctg cagcttcgaa ctccagcacg
     8821 agagaagagc cagtttgggc aaggaccttg cctgccgtgc cgagcaagac agcgcccgcg
     8881 agtccggctc cgccgagcct caagaagtct ctccggtcta tcttccatcg cagatcttcg
     8941 gaatacatgt cccccctatt acttgcttgc aacctcaatt atctctttac taccttgatc
     9001 gcgtcggcga ttatgtagcc ggtgctggag ctcttgctgg aaatcccgac ggaccagctg
     9061 tctccggccg ccatcgtgta ggtgcccagg ctcacccatt tgccgccgtt tgtccgctgg
     9121 ctgacggtct tgttgaccgt tccattgacg gtgcgaatct tgtaagtagc ccgacccgtg
     9181 taacctgagt tggacggcca ccaaccgtac accgtgtagc tgccgcgcga cgggatcttg
     9241 accttgtatt gcgcgggatc gtagaccgac aggggcttca agagcgccct gtagttgtta
     9301 ccgtaacgct gcgagctgaa ggagctgttg gtccacttgc tcgaagcccg gaaccgctgg
     9361 gcgctgctgt tgtcgatgat ctgaacgttg gggctggtcg ttgcggtaga ggtgcccgcg
     9421 tagctcttga cgaggttcat gtatttggtc cagtcccaat atggtccggg atctgtgtgg
     9481 cagtcgcctc ctccgccaac gccggagcag ccgggcacct cgttatgccc gataatgtgc
     9541 gtacggtcga tggggatgtt gtacttcttt gcgaggtagg ccgagagccg ggccgaagac
     9601 ctgtacatgg cgtcggtgaa ccacgacggg tcgcttataa aaccctcgtg ctcgatgccg
     9661 atgctgtggg tgttggtcgg ccaccatcca gcgtgccagg cgatgtcatc ctgatgaacc
     9721 gactggccta tgaagccgtc ggaagagcgc acggtatagt gagcggacga tccggtgttg
     9781 ctggggctcg cgaaccagtt tatggcgctt gaccatgagc cctgcgtcac gtggattacg
     9841 atggtgttta tgtcgtagga ggcttctctg ctggaggggg tgtagttcga accgccgttc
     9901 ccgtaccagg tagagcccga ataatcgccg gtcgcctgcg cggtgaatag ttgcggaaca
     9961 tcgacgttct ggggcgccag atctaccttc tctcccgtcg agatagttgc ggaggcaccg
    10021 cttttcagca ctttgtagac ctgatccgcg tactgcccgc tgcctccgta cttgctaacc
    10081 gcatcgtacc agccactcag gtcggaaggc ttctgcgagc cctggatgtc cgcgaggacc
    10141 gccgccccgc cccggatgtt cgcatcgcgc tcactcttga tctggtcctg agatagtccg
    10201 gttagagacg cagccttccc cagagtatcc ctcgaagggt tctgtaccag cgccatgaca
    10261 ccgtatgtgc cccggccttc gggatcgttc ttatcgtagt cgctggcagt gggaggcggc
    10321 atctcccaat ggctgttgac gtatcccatg gcaaggagca actccttcgg gacgttcgac
    10381 tcattcgctg ccgaaccgaa ctcgtcagag atggacgcct gctgggccgt cgcgtgcgag
    10441 ctgggaccaa ctaatcccat gattgcggcc aaaccaagag tcgcaagaac tataattcct
    10501 gaacgcaaat ctccccctcc gagaggataa tacagttgtt tctacctcta ttatcgacca
    10561 tcgtggcaga aaacttaagt attcaaggaa acattttcga aacatattct caaaaatgtt
    10621 tcgatttcga aacatcgtat gtaaatggtc tcatctacga gattgaaaca cacgatgacg
    10681 cgactactca aagcaaggtt tttgtgcgaa catatggcgc gttgattttg gatatgtata
    10741 gatatatcaa cgggagtaga tctggtgccg cccttgaaag actgtcccca tcatgaactg
    10801 ccatggggaa taaagagatg acgcgaaact cgggcgtgcc aggatacaag ccctccacgt
    10861 tgacgagaat aaagggagtc ttgcggcgtg tagtcggcag gggccggagg cttgcaatca
    10921 gtaagcgtat cccaaaggac ggggcggaga cacgggttag accggagaac gttgtctgga
    10981 tcttcggctc cggccgaacc ggaagtacct ggcttgctgc gatgatgggg gagatagagg
    11041 gtcagaccgt atggcgcgag cctctggtgg gagcgctctt cggtaacctc tactacgtac
    11101 gggccgaaca tcgcatagac aaacgcggtg agcatttcat cctgggcgga gcgtacaagg
    11161 acacctggat ggggccgatg agggagatgg tcctgggtgg agccgcagcc aggtttccgg
    11221 aattgacccg tggcgggtac ctggtggtca aggaaccgaa cggctccatc ggggcaccac
    11281 ttctcatgga ggccatgccg gagagccgga tggtgctctt gatccgggac ccgagggacg
    11341 tcgtagcctc cagcatggac gcgcgccgtg aaggaagctg gctgcacgag aggcgcaaca
    11401 cggatagccg gaagcgagag tccctaccgg acaagaaccc caacgtctat gtcaagacgc
    11461 gggcgaagac ctacctccag cacgcgggca acgccaagag agcctacgac gcacaccggg
    11521 gatacaagac ggtggtccgc tacgaggacc ttcgggcaga caccctggcg acgatgaagc
    11581 gcctctacgc cgagttgggt attccggttg atgaggaggg cctcatccgg gcg
//
LOCUS       DASSMB010000074        15273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1411058, whole genome shotgun sequence.
ACCESSION   DASSMB010000074 DASSMB010000000
VERSION     DASSMB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15273
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411058"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1721)
                     /gene="glmS"
                     /locus_tag="VFJ72_04935"
     CDS             complement(<1..1721)
                     /gene="glmS"
                     /locus_tag="VFJ72_04935"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET7478844.1"
                     /translation="MCGIVGYVGRERCVEVLMEGLRHLEYRGYDSAGLALAVPGLDEV
                     AQVKRVGPLKELAEAVSQHNGHFANVRTGVGHTRWATHGRPTEANAHPHTGGYAGSDG
                     DGIVAVVHNGIVENHVELKEELEQRGRVFRSETDTEVIAHLLAERVEDGEPLREALTN
                     TLKRLRGSFAVAAVSAKEPEKVVAARHQSPLVVGLGDGENFIASAVQALLGETHRFMV
                     VENGEVVEITGSSASIYTMDGDPVERESFEVDWDAEAVELGGYEDYMLKEIHEQPAAL
                     QATLAGRLDADGTVNLSEMGLDLSNVDRIVIVACGTAYHAGLLGKYAIEKFARLPVEV
                     AVASEYRYSDPIGDEKMLVVAISQSGETTDTLAAVEAAREFGGRVLAVTNTQGSLITR
                     EADSVLLTKTGPEIAVASTKTFLAQATLLYVLALELASLRGTLQKEEVAQIGRALRQA
                     PEKVDETLNLLHGRVEEAAELFEEALCALFLGRGASFPVALEGALKLKEISYIPAEGY
                     PAGEMKHGPIALIDERCPVVAVLGEGLLREKTLSNVEETVARGASVIAIARQDDPDAE
                     RIARVVLP"
     gene            complement(2113..2922)
                     /locus_tag="VFJ72_04940"
     CDS             complement(2113..2922)
                     /locus_tag="VFJ72_04940"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HET7478845.1"
                     /translation="MIPSSVGEECGTQGEATKTVMRKPGRLWEVDAARGIAILMMAFY
                     HLTYDLDTFGGYAIESTSGYWALFADTTASLFLFLVGVSLTISYIRAGGESAGNLFGK
                     YLLRGLRVFGYGMVLTLVTWVAGVGIIVFGILHLIGVSIILAYPLLRFRLPNLVLGVV
                     MIVVGEYMRLQGVSSESPLLLPLGVVPESLFMPDYRPLLPWFGVVLLGVFFGNLLYGR
                     GYAERRAGGESPVAARPLGFLGRHSLLIYLVHQPVIIALLAVLGIIEFGGL"
     gene            2972..3457
                     /locus_tag="VFJ72_04945"
     CDS             2972..3457
                     /locus_tag="VFJ72_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478846.1"
                     /translation="MAEREFVIRRGRREDAPAAARLWVQSAREHAAYDPVYNTAPEAE
                     RTMRRFLADLASSSHSFLLVAVANPEERVIGFISGELREGSPTFHSKTWASVDDVFVE
                     EGYRSLGVGRALIEGVRSWAEERGAHGVSLQVAAANARGRKFYEDLGFREVSVYEVLE
                     F"
     gene            complement(3496..3936)
                     /locus_tag="VFJ72_04950"
     CDS             complement(3496..3936)
                     /locus_tag="VFJ72_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7478847.1"
                     /translation="MNIRVTLIDRRDLAALLALLEESLRNGEPVPEAFAELLAGAVES
                     GDLEVLGAYAEGGPVGVAILAYRLNVAAGGLFASIEDLYVRPEVRRQGVGRALMRSVK
                     QRCAARRVSYVEVEVEDGSAEAFYAACGYEREPDVRVMSVSHVL"
     gene            complement(3939..4958)
                     /gene="ltaE"
                     /locus_tag="VFJ72_04955"
     CDS             complement(3939..4958)
                     /gene="ltaE"
                     /locus_tag="VFJ72_04955"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HET7478848.1"
                     /translation="MIDLRSDTVTRPTEGMRRAMAEAPVGDDVFGEDPTINRLEEYVA
                     ELLGKEAAIYAPSGTMTNQIGVHVGTGRGEEVLLHEGAHIFNYEAGAPALISSVQVRT
                     LPGERGVISSETLRGAIRPENVHFPRQTLLCLENTHNMEGGTVFPLEEFAAVAAEAHD
                     LGMKVHLDGARLFNAQAATGTPASEWCRHADTVSVCSSKGLGAPVGSLLAGDEDTIRE
                     ARRARKAFGGGMRQAGIIAAASLYAFEHNIDRLADDHERARALADGLAEAGYSVDPPE
                     TNIVLVEVGDADAFLRSLALEGVLATPRTASSIRLCTHLDVTDRDIQDAVEAAARVRA
                     ATGAN"
     gene            complement(4958..5911)
                     /locus_tag="VFJ72_04960"
     CDS             complement(4958..5911)
                     /locus_tag="VFJ72_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET7478849.1"
                     /translation="MTPTPKHAIPKDLSRLLAASGVRVAGHREIDYGTQYRLARGPDT
                     ANLNVYRTGRVSVDGKASELKTLLENWRVANSAPKAGRPANAMNGASFPAASGVARLG
                     IDEAGKGEYLGPLVVAGVRVLGESQDRKLREAGVRDSKDLSVAQAREVSGLVKRILGE
                     ENFRILSLEPRTYEERRDAAGRNVNRLLGELDAEILDELAVDVEVVILDSFGPKARSY
                     VEPRVPPGVRLEVRPRAEDDAAVAAASILARARYLEEMERLSGRAGFELPRGSTHVLD
                     AARRVYRERGMEGLRDVAKVHFATTEQVTGGSGTRVKRGRA"
     gene            complement(5908..6201)
                     /locus_tag="VFJ72_04965"
     CDS             complement(5908..6201)
                     /locus_tag="VFJ72_04965"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7478850.1"
                     /translation="MTYDTGNDRQRAHVYVSGGVQGVFFRESARQKAEELGIAGWIEN
                     LPDGRVEAVFEGSPEDVRRMTEWCEQGPTYATVEDVQTELEDARGDLRGFEVR"
     gene            6332..7876
                     /locus_tag="VFJ72_04970"
     CDS             6332..7876
                     /locus_tag="VFJ72_04970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7478851.1"
                     /translation="MGERGGLRDDSRSSGRTQITLVVPTRNEADNVPRLVRELRSGLT
                     GIDYRVVFVDDSTDHTPQVIRELAAEDERVTLIHREGTEREGGLSTAVTTGMDTVSHD
                     SEYTCVMDADLQHPPEKVREMLEFARESGADVVVASRYAPGGSYAGLSGPVRRAVSVG
                     SKYLAQVVFKEARKTSDPMTGFFLIKNEAISGIQFRPTGFKILLEILVCAPELKVVEA
                     PFNFQARNAGVSKASMRQGLDYLSHILSLFWYVPAAGRFWKFALVGASGVLVNILTLV
                     VLAEFFNASKVIAWMFGVGLSILSNFLLNNAFTWRDIRHSSHIHFLLRGALAYPVAIV
                     GIGANFAVYYPLLKYVTDAFPYYVIFSFLGIVAATGVNFILSSRLIFRPSLPKSLDPE
                     APPKEIARDIQRELKADWVGLIRLPDLTPIGEPNHRDLTTADRSVVDMVISTNHPTLA
                     VTGPRRLPQARTNTRWTNSLAVPVLRGEQIVGVVYATRNSTEPFTEDDLHWLTAYAST
                     AAPLFD"
     gene            8416..9510
                     /locus_tag="VFJ72_04975"
     CDS             8416..9510
                     /locus_tag="VFJ72_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HET7478852.1"
                     /translation="MLQAKRVDELVNKLVELGGSDLHLKVGAAPSVRVHGGLAALEGY
                     DQLAPEDTEEILREIIPAQLTGEFESEGEADFSYAVAGLGRFRVNAFRQRGSVSIVMR
                     FIPIGIPRFEDLRLPEAIRTLSKEERGIILVTGTTGSGKSTTLASMINLINHTSAKHI
                     VTIEDPIEFLHKDAQSLVNQREVGTDTASFARALRRVLRQDPDIILIGEIRDSETAQI
                     ALSAAETGHLVLSTLHTVDATETVNRIIDLFPPHERDGVRAMLAGTLKGIIGQRLVRT
                     TDGKGRAAACEVMVTTGRIRDFIMDPDQTGQISEAIAEGEYYGMQTFDQALLKLVEEE
                     RVDYREALQASSRPQDFKLMVQSLGLGMGR"
     gene            9625..10596
                     /locus_tag="VFJ72_04980"
     CDS             9625..10596
                     /locus_tag="VFJ72_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HET7478853.1"
                     /translation="MDLGGSVLQSWEWGEFRQKHGWKSLRLISDGGDAAAQVLLRELP
                     LPGLGSLAYVPHGPLCADESSFVNVVESVSDHARKSGAALIRVEPRVSEEQGFEARGF
                     TRSDSSVQPRCTRLLEVLEDPEEQLKALPKDTRYGVRRAGREGVVAGPSARPESDLEA
                     FLDLLEETARRQNFATRTREYYRDFLDTLPAQLIVARKEGDERLLAGAMILTFGAEAY
                     YLYGASAADSENLYASYLVQFEVLRAARQAGATRYDMWGPCKPREGDPLWGVYQFKKK
                     FGGREESYVGAHEKDLSPLRARAARAGIKGYYAVQKLRGKSSGPISD"
     gene            10660..11490
                     /locus_tag="VFJ72_04985"
     CDS             10660..11490
                     /locus_tag="VFJ72_04985"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HET7478854.1"
                     /translation="MRPICFLSDFGLADDFVGTCKGVMLRIAPGVSIVDLTHQIPGFG
                     VEIGSEFLAHATHYMPDDTVYLAVVDPGVGTERRAIALRARSGALLVGPDNGLLIPAA
                     DSLGGISEAVLLTNDGYHVHPISSTFHGRDIFAPSAAHLAAGVELANLGEAVEPSSLT
                     SFDPPGVEVSSDGRSMKARIMDIDRYGNARLSILQKEAGLEYGDTLTVDAGDGSMGVR
                     YLETFGAAKSGELVIVPDSHWRLSLAINTGNAAHALALKVGGTVRLERTDDGARDASP
                     "
     gene            11465..12253
                     /locus_tag="VFJ72_04990"
     CDS             11465..12253
                     /locus_tag="VFJ72_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478855.1"
                     /translation="MEREMPAPERLPSAPTPLSPDDRRKVTRAALLRPMNLLMLLIGV
                     VVFVAALPATSLAWLLLPLTLVTYASLAFLSSRDPNLRQKVLGEGATSPIAPVRNNAT
                     NSDVSPDRRARWLPRGETREKVEAALASYRSTIKAIEESDDVTREVLEDAVPKLHAAA
                     DRLVDVAHRREKAASTVRELRSGRTDEEREATLREIEEEVNKADAEISATSEQFLTLR
                     ARIVRVSLDATAATDLNSSLDELNLRLEALASTMSPARDTTGDL"
     gene            complement(12385..12942)
                     /locus_tag="VFJ72_04995"
     CDS             complement(12385..12942)
                     /locus_tag="VFJ72_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478856.1"
                     /translation="MREDMEYINGVLDELDAIVREASGVPMRKGRAVVDRADLLVMLD
                     ELRGSLPPELSEAESIRREGGAIVSAAHEEAARIVEEAHHRADSMVEETEPYRRALRR
                     ADEVIERAERYGEEISGGSEVYRDRVMGQLENWFQDSLVSVGESRQELSGAPVHRPEP
                     QPVRQPVPDAYQENGDEQRWRASSA"
     gene            complement(13184..>15273)
                     /locus_tag="VFJ72_05000"
     CDS             complement(13184..>15273)
                     /locus_tag="VFJ72_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7478857.1"
                     /translation="LMMVDGSTAAGVLREVGLTEADVRRILGKMRGEGGLGEDPDSLL
                     AKHGCDITRLAREGKLDPVVGREDEIKRVIQILARRTKNNPALIGEPGVGKTAIVEGL
                     AQKIATGYVPEVLKDRVIFSLDLGSLVAGTKFRGEFEERLKGVIDEVKSAYGRIILFV
                     DEFHNLVGAGAAEGSMDAGEILKPALARGEVQVIGATTIDEYRRHVETDAAFERRFQP
                     VLIEEPTEEETVQILAGIKDRYEAHHGVEISDEALKAAAQLSHRYIADRFLPDKAVDL
                     LDEAASQVRIETTMMPDELRELELRLQELTREGAAAVEDRDYEQAALLRAEADTLQAE
                     FREARERWMEEAGVREAVVGREEIARVVSARTGIPVTRMLKDEAERLLSMEDALHRRV
                     VGQDAAVVAVAEAVRKARAGLANPNRPIGSFIFLGPTGVGKTELARALAEYLFDDEDA
                     MIRLDMSEYMERHAASRLVGAPPGYVGYDEGGQLTEAVRRRPYSVVLLDEIEKAHPDV
                     FNMLLQLLEDGRLTDNKGRTVSFDNTVVVMTSNAGAHLIPGAEEMREREEEIRDRLMQ
                     ELQTFFRPEFLNRVDEIIVFHSLSDKELVEISTLLLENLRKTAAEQDIDLQFSDAVAR
                     RIAEEGRNSRFGARPLRRAIGKLIESPLSKEIIAGRINPGYTVRVDLDASGTLTFERA
                     DAPASTSVTPEAS"
BASE COUNT         2689 a         5221 c         4709 g         2654 t
ORIGIN      
        1 ggtagcacca cgcgggctat gcgttcggcg tccgggtcgt cctggcgggc tatggctatg
       61 acgctggcgc cgcgggccac ggtctcctcg acgttggaga gggtcttctc gcgcaagagc
      121 ccctcgccca ggacggcgac cacggggcag cgctcgtcga tgagggctat gggaccgtgc
      181 ttcatctcac cggccgggta tccttcggcg gggatgtagg agatctcctt gagctttaga
      241 gccccttcta aggccaccgg gaaagacgcg ccgcgcccga ggaacagcgc gcagagcgcc
      301 tcctcgaaga gttccgcagc ctcctcgacg cggccatgca acaagttcag cgtctcgtcc
      361 accttctccg gcgcctggcg cagggcgcga ccgatctgcg ctacctcttc cttctggagc
      421 gtgcctcgca gcgaggctag ctccagggcc aggacgtaca ggagggtcgc ctgggcgagg
      481 aaggtcttgg tggaggcgac cgctatctcc ggcccggtct tcgtgagcag tacggagtcg
      541 gcctcgcggg tgatcagtga gccctgcgtg ttcgtcacgg ccagcacccg tccgccgaac
      601 tcccgcgcag cctcgacggc ggccagggtg tctgtggtct ccccgctctg gcttatggct
      661 acgacgagca tcttctcgtc gcctatcggg tcagagtagc ggtactcgct ggcgacggcc
      721 acctcaacgg gcaggcgggc gaacttctcg atggcgtact tgccgaggag cccggcgtga
      781 taggccgtcc cgcaggccac gataacgatg cgatccacgt tcgagaggtc taaccccatc
      841 tccgagaggt ttaccgtccc gtcggcgtcc aggcgccccg cgagcgtcgc ctgaagggcc
      901 gcgggctgct cgtggatctc cttgagcatg tagtcctcgt agccgccgag ctctaccgcc
      961 tcggcgtccc agtcaacctc gaagctctcg cgctcgaccg gatcaccgtc catcgtgtag
     1021 atactggccg aagagcccgt gatctccacc acctcgccgt tctccacgac cataaagcgg
     1081 tgcgtctccc caagcagcgc ctgcaccgcg ctggcaatga agttctcgcc gtctccgagc
     1141 cctaccacca gggggctctg gtgccgcgca gccaccacct tctcaggctc tttggcgctc
     1201 accgccgcga ccgcgaacga gccccgcagc cgtttcagcg tattcgtcag ggcctcgcgc
     1261 aacggctccc catcctccac ccgctcggcc agcaggtgcg cgatcacctc ggtgtccgtc
     1321 tccgagcgga agacacgacc ccgctgctcc aactcttcct taagctccac atggttctcg
     1381 acgatgccgt tgtggaccac cgcgacgata ccgtccccat cgctacctgc gtagcccccg
     1441 gtgtgaggat gagcattcgc ctcggtggga cggccgtgcg tcgcccacct cgtgtgcccc
     1501 accccggtcc tcacgttagc gaagtgtccg ttatgctgcg agacggcttc ggccagctcc
     1561 ttcagcggac cgacccgctt tacctgagcc acctcgtcca ggccagggac ggccagggca
     1621 agtcccgccg agtcgtagcc gcggtactcc aggtgcctaa ggccctccat cagcacctcg
     1681 acacaacgct ccctgccgac gtaaccaaca atcccgcaca tgcgtcaacc tccctctaat
     1741 cgtccgggga tgcacggtag agaaaggcag aaaaagagac tgggatccac ccgggcccgg
     1801 acggtgtttt gtccccggtg tcgccaccgg attttgtcac cgtcctatcg accgcccgat
     1861 tgccgagacg cacccgccga atgtttcgat gaacgtctgc ctcgtcaact ctccgtgagg
     1921 agagtcctgg cgctttggtt tttgtctctc cgccgatctc gcaaaccgat gcctgctacc
     1981 cggttcacca aacctatcta cgacacgagt ctctcttctg aactaacctc ccgacttcga
     2041 agaacaccca acgatagata ctttacctgc tctaaggtag aaagaaaacc cttacgccga
     2101 aaccgcagcg gctcagagcc ccccgaactc tatgatcccc aagaccgcta acagagcgat
     2161 aattaccggc tgatggacca ggtagatcag taacgagtgc cgtccgagga accccagggg
     2221 cctagccgca accggactct cgcctcccgc ccgcctctcc gcgtagcccc ggccgtagag
     2281 caggttccca aagaagacgc ccagcaggac cacgccgaac cagggcaaaa gaggccggta
     2341 gtccggcatg aacaggctct ccggcacgac gcccaggggc aggagcaagg ggctctcgga
     2401 ggagaccccc tgcagccgca tgtactcgcc aactacgatc atgacgacgc ccaagaccag
     2461 gttcggcagc cggaacctca gtagcgggta cgcgaggatt atggagaccc cgatcaagtg
     2521 cagtatcccg aacacgatga tgcccacacc ggcaacccac gtcacgagcg tcaggaccat
     2581 cccgtaaccg aagaccctga gcccccgcaa aaggtacttg ccgaagaggt ttcccgcgct
     2641 ctccccgccc gccctaatgt agctgatagt gagcgagact cctaccagga acaagaacag
     2701 gctcgcggtc gtgtcggcga agagcgccca atacccgctc gtggactcga tggcgtagcc
     2761 gccgaaggtg tccaggtcat acgtcaggtg gtagaaggcc atcatgagga tggctatgcc
     2821 ccgcgcggcg tccacctccc acagacgccc cggcttcctc atgaccgttt ttgtagcttc
     2881 gccctgcgtc ccgcactcct ctccgaccga cgaggggatc atacctcagc gcacatccga
     2941 catgccgcgc catatccgct aggattccgg catggccgag agagagttcg tcatccgacg
     3001 tggacggcgc gaggatgccc ccgctgcggc ccggctctgg gtgcagagcg cccgcgagca
     3061 cgccgcctac gaccccgtct ataacaccgc tcccgaggcg gagcgcacca tgcgccgctt
     3121 cctcgcggac ctggcgagca gcagccactc cttcctgctg gtggccgtcg ccaaccccga
     3181 ggagcgcgtg atcggcttta tctccggcga gttgcgcgag ggctccccta ccttccactc
     3241 gaagacctgg gcctccgtgg acgacgtctt cgtcgaggaa ggctaccgca gcctcggcgt
     3301 cgggcgcgcc ctgatagagg gcgtcaggtc ctgggccgag gagcgcggag cccacggcgt
     3361 ctcactacag gtcgccgccg ccaacgcccg cggccggaaa ttctacgagg acctcggttt
     3421 ccgcgaggtc tccgtctacg aggtattgga gttttagaga gctttcactg atcagcgaga
     3481 gctggaagcc gggtctcaaa ggacatgaga caccgacatg acccgcacat cgggctcccg
     3541 ctcgtagccg caagccgcat aaaacgcctc cgcggatccg tcttccacct caacctcaac
     3601 gtaagagact cttcgcgcag cgcagcgctg cttcactgat ctcatcaaag ctctcccaac
     3661 accctggcgc cggacctccg ggcggacgta caggtcttct atgctggcga aaagtccgcc
     3721 agccgccacg ttcaggcggt aggcaagtat ggcgacgccg accggccccc cttccgcgta
     3781 agcgcccaga acctccaggt ctccgctctc taccgctccg gccagcagtt ccgcgaacgc
     3841 ctccgggacc ggctcgccgt tccgcagaga ttcctctagg agagccagca gagcggcgag
     3901 gtcccgcctg tctattagag ttactctgat gttcatgttc aattagctcc ggtggcggct
     3961 ctcacgcgag cggcggcctc cacggcgtcc tgtatatccc ggtctgtcac atcgagatgg
     4021 gtgcagagcc ggatggagga ggcggtccgg ggcgtcgcca gcacgccttc taacgcgaga
     4081 gagcgcagaa aagcatcggc gtcaccgacc tcgacgagca cgatgttggt ctcgggcggg
     4141 tctacggagt atccggcttc ggcgagaccg tcggcgagcg cacgggcccg ctcgtggtca
     4201 tcggcgaggc ggtctatgtt gtgctcgaag gcgtagagag aggcggcggc gatgatcccc
     4261 gcctggcgca tccccccgcc gaacgccttg cgggcgcgcc gggcctcccg gatggtgtct
     4321 tcatcgcccg ccagcaggga gccgaccggc gcgccgagac ccttggagga gcagacggag
     4381 acggtatccg cgtgccggca ccactcgctg gcgggggtcc cggtggccgc ctgggcgtta
     4441 aagaggcgcg cgccatcgag gtggaccttc atgccgaggt cgtgagcctc tgcggcgacg
     4501 gcggcgaatt cttcgagcgg gaaaaccgtc ccgccttcca tgttgtgcgt gttctccagg
     4561 cacaggaggg tctgacgcgg gaagtggacg ttctccggcc ggatcgcacc gcgcagcgtc
     4621 tcggaggaga tgacgccgcg ttcgccgggg agtgttcgga cctgcacgct ggagatcagg
     4681 gcgggcgcac cggcttcgta gttgaagatg tgcgccccct cgtgcaacag cacctcctcc
     4741 ccgcgcccgg ttccgacgtg gactccgatc tggttggtca tcgtcccgga gggcgcgtag
     4801 atggcagcct ccttaccgag caattccgcc acgtactcct ccaggcgatt gatcgtcggg
     4861 tcctcaccga acacgtcgtc cccgacaggc gcctcggcca tcgcccggcg catgccttcg
     4921 gtcggcctcg tcacggtatc actccgaagg tctatcatta agctcgtcct ctctttactc
     4981 tcgttcccga accgccggtt acctgctccg tggtggcgaa gtggaccttc gccacgtctc
     5041 tcagcccctc catgccccgc tcccgataaa ctctcctggc cgcatccagc acgtgcgtcg
     5101 agccgcgcgg cagctcgaac ccggcccgac cggagagccg ctccatctcc tccagatacc
     5161 tggcgcgggc caggatggaa gccgccgcca ccgccgcgtc gtcctcggcc cgcggacgca
     5221 cctccagccg aacccccggc ggcacgcgcg gctctacata agaacgcgcc ttcggcccga
     5281 aactgtcgag gatcacgacc tcaacgtcaa ccgcaagttc atccaggatc tccgcgtcca
     5341 gctcaccgag cagacggttc acgttgcgtc cggcggcgtc ccggcgttcc tcgtaggtcc
     5401 tgggctccag ggagaggatg cggaagttct cctcaccgag gatccgcttc acgagcccgg
     5461 agacctcccg cgcctgcgcg acgctcaggt ccttcgagtc ccgcacccca gcctcccgca
     5521 acttccggtc ctggctctct cccaggaccc gtactcccgc gaccaccagc ggccccaggt
     5581 actctccctt gccggcctcg tctatgccca gccgcgccac gccgctggca gccgggaacg
     5641 aggcaccatt catcgcattt gccggccgac cggctttcgg agcgctgttc gcgacccgcc
     5701 agttctccag aagagtcttc aactctgaag ccttgccatc taccgaaacc ctgcctgtgc
     5761 ggtacacgtt caggttcgcg gtatcggggc cgcgggcaag gcggtactgg gtaccgtagt
     5821 cgatctcgcg gtgcccggcg acgcgcactc ctgatgcggc caggagccgg gagaggtctt
     5881 tagggatggc gtgcttcggc gtgggcgtca acgtacctcg aagcctcgca ggtcgccccg
     5941 ggcgtcctcc agttcggtct ggacatcttc gaccgtggcg taggtcggtc cctgttcgca
     6001 ccactctgtc atgcggcgca cgtcttccgg ggagccttcg aagacggctt ccacccggcc
     6061 atcggggagg ttttcgatcc atccggcgat gccgagctcc tcggctttct ggcgggcgct
     6121 ctcgcggaag aatacgccct gcacgccgcc ggagacgtat acgtgcgctc gctgccggtc
     6181 gttgccagta tcgtaagtca tcgtacctcc ggttgggatc atccagcgcc gatcctagtg
     6241 taccgtttgt gtgggctctt tcttgcggat atgctagcct agagcggtta tgagagcgga
     6301 cgaccgagcg gtgggtggac ggagtgcgag cgtgggcgag agaggtggcc tgcgtgacga
     6361 ctcaagaagt tcggggagga cccagatcac gctcgtcgtc ccgacgcgca acgaggcgga
     6421 taacgtcccg cggctggtgc gcgagctgag gagcgggctg accgggatcg actaccgcgt
     6481 ggtcttcgtt gacgactcta ccgaccatac gccacaggtc atccgcgaac tcgccgccga
     6541 ggacgagcgc gtcaccctga tacaccgcga aggcacggag cgggagggcg gcctctcgac
     6601 ggccgtcacc accgggatgg acaccgtatc gcacgacagc gagtacacct gcgtaatgga
     6661 cgcggacctc cagcacccac cggagaaggt gcgggagatg ctggagttcg cgcgcgagtc
     6721 cggcgcggac gtggtggtgg caagccggta cgcgccgggc ggcagctacg ccgggctctc
     6781 cgggccggtg cgtcgggccg tctccgtggg gagcaagtac ctggcgcagg tcgtcttcaa
     6841 ggaggcccgc aagaccagcg acccgatgac cggcttcttc ctcataaaga acgaggccat
     6901 ctccggtatc cagttccggc cgacggggtt caagatcctg ctggagatcc tggtgtgcgc
     6961 cccggagttg aaggtcgtcg aggcgccgtt caatttccag gcccgcaacg ccggcgtctc
     7021 gaaggcgagc atgcgccagg gtctcgacta cctgagccac atcctgagcc tgttctggta
     7081 cgtgccggcg gcgggccgct tctggaagtt cgcgctcgtc ggcgcgtcgg gagtgctggt
     7141 caacatcctg acgctcgtag tcctggccga gttcttcaat gcgagcaagg tcatcgcctg
     7201 gatgttcggc gtcgggctct ctatcctgag caacttcctc ctgaacaacg cctttacctg
     7261 gcgggacatc cggcactcca gccacatcca cttcctgctg cgcggcgctc tagcttaccc
     7321 ggtcgccatc gtcgggatcg gggcgaactt cgccgtctac tacccgctgc tcaagtacgt
     7381 cacggacgct ttcccttact acgtgatctt cagcttcctc ggcatcgtcg cggccacggg
     7441 cgttaacttc atcctctcct cgcggctgat cttccgcccc tcgctgccga agagcctgga
     7501 cccggaggcg cctccgaagg agatcgcccg cgacatccag cgcgagctga aagcggactg
     7561 ggtcggcctg atacggctcc cggacctgac cccgatcggc gagccgaacc accgcgacct
     7621 caccaccgcc gaccggagcg tcgtggacat ggtcatcagc accaaccacc ccaccctcgc
     7681 cgtgaccggc ccgcgccgtc tgccgcaggc ccgcacgaat acccgctgga cgaactccct
     7741 cgccgtgccc gtgctgcgcg gagagcagat cgtcggcgtc gtctacgcga cccgcaactc
     7801 caccgagcca ttcacagagg acgacctgca ctggctcacc gcctacgcca gtaccgcggc
     7861 ccccctcttc gactgagacc ccgtactctc ataacggcaa acgccccggc gaagaccgtc
     7921 cgccggggca ccggctcccc cccataagga gcctcatatc ggacctcacc accccctccc
     7981 gccggtgagg ccccgcacag atcccacgct tcaacccaag ctttaaaccg agccccgggt
     8041 ccgcaagacc tgctgatgcc aaaattagca ccacccctac taccgcgcaa cccaaagaaa
     8101 accctatacc taagccaaat tcgccaacac tctctccacc gcgcacgata tgtcctttac
     8161 ctgagcccca cgcacactac atcttgagtc tcaacccgag tcaaaccgtg caacgaaatt
     8221 cacatgaacc attagtacaa ggtgaggtta gtttaaggtc gagattcaac tattggcacc
     8281 cggagcgggc ttctggcgct tatatatggg ttttctgtag gcgttggtat ttgaggagga
     8341 tgagactaaa gtgtgtaggg ctggcggtcg aagaatgaaa gaacggctaa cgttcgtaga
     8401 gaaagggtgt ttgggatgtt gcaggcgaag agagttgatg agttggtaaa caagctcgtc
     8461 gagctgggcg gcagcgacct tcatctgaag gtcggcgcgg cgccgtcggt gcgggtacac
     8521 gggggattgg ccgctctgga agggtatgac cagctagcac ccgaggacac ggaggagatt
     8581 ctgcgcgaga taataccggc gcagttgacc ggagagttcg agagcgaggg agaagcggac
     8641 ttctcctatg cggtggccgg gctgggacgt ttccgggtca acgctttccg gcagcgaggc
     8701 tccgtctcta tcgtgatgcg gttcataccc atcggtattc cccggttcga ggacctgcgg
     8761 ctgccagagg ccatacgcac gctctccaaa gaggaacggg gcatcatcct cgtcaccggc
     8821 acgacgggca gcgggaagtc cacgacgctc gcgtcaatga tcaacctgat caaccacacc
     8881 agcgcgaagc acatcgtcac catcgaggac ccgatagagt tcctgcacaa ggacgcgcag
     8941 agcctcgtta accagcgcga ggtcgggacg gacacggcgt ccttcgcgcg ggccctgagg
     9001 cgagtgctgc gccaggaccc ggacatcatc cttatcggtg agatccggga ctccgagacc
     9061 gcccagatcg ccctctccgc cgccgagacc gggcacctcg tcctctctac tctgcacacg
     9121 gtagacgcga cggagacggt gaaccggatc atagacctct tcccgccaca cgagcgggac
     9181 ggtgtgcggg cgatgctggc gggcaccctc aaggggatca tcgggcagcg cctcgtgcgg
     9241 accacggacg gcaagggccg ggcagcggcg tgcgaggtca tggttaccac cgggcgcatc
     9301 cgggacttca tcatggaccc ggaccagacg ggccagatct ccgaggcgat agccgagggc
     9361 gagtactatg ggatgcagac cttcgaccag gccctcctca agctggtgga ggaggagcgg
     9421 gtggactacc gggaagccct gcaggcatcc agccggccgc aggacttcaa gctcatggtc
     9481 cagtccctcg gtctcggcat gggacggtga gaccgtaggg gtttgccaac cgccggagct
     9541 ttcccgataa ttgcgccgac accgatcagg aggttgactg tccttacaga agctcacaac
     9601 cgtgaaacgt ggaacagctc tgtcgtggat ctcggcggga gcgtgctcca gtcctgggag
     9661 tggggagagt tccgccagaa gcacggctgg aagtccctgc gcctgatctc cgacggcgga
     9721 gacgcggctg cccaggtgct gctgagggag ttgccgctgc cggggctcgg atctctggcc
     9781 tacgtccccc acggtccgct ctgcgccgac gaatcgtcct ttgtgaacgt cgtggagtcc
     9841 gtctcagatc acgcgcggaa gagcggcgcg gccctgatcc gggtcgagcc ccgcgtgagc
     9901 gaggagcaag gcttcgaggc gagaggcttc acgaggagcg atagctccgt acagccgcgc
     9961 tgcacgcgac tcctcgaagt tctcgaagac cccgaggagc aactgaaagc cctcccgaaa
    10021 gacacccgct acggcgtccg gcgcgcaggt cgcgaaggag tcgtggccgg tccttccgcc
    10081 cggccggagt cggacctgga ggcatttctc gacctgctgg aagagaccgc ccggcggcag
    10141 aacttcgcaa cccgcacccg cgagtactat cgggacttcc tcgataccct cccggcgcag
    10201 ctcatcgtgg cgcgcaagga gggcgatgag aggcttctag caggagccat gatcctgact
    10261 ttcggcgccg aggcgtacta cctctatgga gcgtccgccg cggacagcga gaacctctac
    10321 gcctcttacc tggtgcagtt cgaggtcttg cgggccgccc ggcaggcggg ggcgacgcgc
    10381 tacgatatgt ggggtccctg caagccgcgc gagggagacc cgctgtgggg cgtgtatcag
    10441 ttcaagaaga agttcggcgg acgcgaagaa agctacgtcg gcgcgcacga gaaagatctc
    10501 agcccgctcc gcgcccgcgc cgcccgcgcg ggtatcaagg gctactacgc cgtccagaag
    10561 ctgcgcggca agagttccgg cccgatcagc gactagcgga ctccggagtc ctaacgcggt
    10621 ccagcggccc cggagcaagc gccgttttat aatattgata tgcggccgat ctgctttctc
    10681 tcggacttcg ggctcgccga cgactttgtc ggcacctgca agggcgttat gctgaggata
    10741 gcccccggcg tctctatagt ggacctgacc caccagatac cgggtttcgg ggtggagatc
    10801 ggctccgagt tcctggccca cgcgacccac tacatgcccg acgacaccgt ctatctggcc
    10861 gtcgtggacc ccggcgtcgg caccgagcgg cgcgccatcg ccctccgtgc ccggagcggc
    10921 gcgctcctgg tcggcccgga caatggtctt cttatcccgg ccgcggactc gctcggcggg
    10981 atctccgaag ccgtgttgct tacgaatgac ggctaccacg tccaccctat atcgagcacc
    11041 ttccacggcc gcgacatctt cgctccgtcg gcggctcacc tggcggctgg cgtggaactc
    11101 gcgaacctcg gcgaagcggt ggagccgtct tcgctcacga gcttcgaccc gccgggagta
    11161 gaggtcagca gcgatggccg gagcatgaag gccaggatca tggacataga ccgctacggc
    11221 aacgcccggc tctcgatcct ccagaaagag gccggcctgg agtacggcga caccctgacg
    11281 gtggacgccg gagacggcag catgggcgtt cgttacctgg agacgttcgg ggcagccaag
    11341 agcggcgagc tggtgatcgt ccccgactcc cactggcgcc tgagcctggc gataaacacc
    11401 ggcaacgccg cccacgcact cgcgctgaag gtcggcggca ccgtgcgcct ggaacggacc
    11461 gacgatggag cgagagatgc cagcccctga acgtctaccc tccgcgccga ctcccctctc
    11521 tccggacgac cggcgaaagg taaccagggc cgcgcttctg cggccgatga acctgctgat
    11581 gctccttatc ggggtcgtgg tcttcgtggc agccctgccc gcgacgtctc ttgcctggct
    11641 gctcctccca ctcaccctcg tcacctacgc ctctctcgca ttcctctcct ccagagaccc
    11701 caacctccgg caaaaggtcc tgggcgaagg cgctacttcc ccgatcgccc cggtcaggaa
    11761 caacgccacg aattccgacg tctccccgga ccgccgcgcc cgctggctcc cgcgcggaga
    11821 gacgcgcgag aaggtcgaag cggccctggc gagctaccga agcaccatca aggccatcga
    11881 agagtccgac gacgtaacgc gagaggttct cgaagacgcc gttccgaagc tgcacgcggc
    11941 ggcggaccgg ctggtggacg tggctcaccg ccgggagaag gccgcgagca cggtgcgcga
    12001 gctacgctcg ggaaggacgg acgaagaacg tgaagccacg ctgcgcgaga tagaagaaga
    12061 agtaaataag gcggacgccg agatctccgc tacctccgaa cagttcctga ccctgagagc
    12121 ccgcatcgtc cgcgtctcgc tcgacgcgac cgccgccacg gacctgaact cttccctgga
    12181 cgagctaaac ctgcgtctgg aagcgctcgc atccaccatg tctcctgcgc gggataccac
    12241 aggagacctc taacccgcga ggttgcgcag tccgaagaac tagagaggat ccgcagtacc
    12301 accccggcgg gtggcgcggt atggtcacgg acgcgctaac gaagcagcgt cgagccgatt
    12361 gatctcgcgc cgcgggaagt tacttcaggc ggagctggcg cgccagcgct gctcgtcgcc
    12421 gttctcctgg taagcatccg gcacgggctg gcggacgggc tgaggctcgg gacggtgaac
    12481 cggcgcgccg ctaagctcct ggcgcgactc tcccacggac accagagaat cctggaacca
    12541 gttctcgagc tggcccatca cccggtcccg gtatacctcg gaaccgccgg agatctcctc
    12601 tccataacgc tccgcccgct ctattacctc gtcagcgcgc cgcaacgctc gccggtacgg
    12661 ctcggtctct tcgaccatcg agtcggcgcg gtggtgcgcc tcctccacta tgcgagcagc
    12721 ctcctcatga gccgccgaga ctatcgcgcc gccctcacgc cttatggact cggcctccga
    12781 gagctccggt ggcagagacc cgcgcaactc atccaacata acgagcaggt cggcgcggtc
    12841 aacgaccgcc cggcccttcc gcatcggtac gccggacgcc tcccgaacga tggcgtcaag
    12901 ctcgtccaat actccgttta tgtactccat gtcctctctc atgcgaccaa ccctagcacg
    12961 cttattcagg ctacgctagg ctttctgaaa gtatttttcg gagctttacc taaagttaag
    13021 gtccaatata atcttaaggt taagtgtaat tgtccctgcg cctaaagttg gccctttgcg
    13081 cggccttagc gagcgagatc ggcccgcttt tataggctgt tttgaaagtt atccacaggc
    13141 ctgctgcgac gctccgaagg ctaaaccccg cgtagaggcg gcgttatgag gcttccggcg
    13201 tcacagaggt ggaagccgga gcatccgccc tctcgaaggt cagtgtaccg gaagcgtcca
    13261 ggtccacccg aacggtgtat ccggggttta tgcgaccggc gatgatctcc ttgctcagcg
    13321 gagactcgat aagttttccg atagccctcc gtagcggacg cgcgccgaag cgcgaatttc
    13381 ggccctcttc ggctatccgg cgggcgacgg cgtcggagaa ctggaggtct atgtcctgtt
    13441 cggcggcggt cttgcggagg ttttccagga ggagagtgga gatctcgacc aactccttat
    13501 cggagaggga gtggaagacg atgatctcgt ccacccggtt caggaactcg ggccggaaga
    13561 acgtctgaag ctcctgcatg aggcggtccc ggatctcctc ctccctctcg cgcatctcct
    13621 cggcgccggg gatgaggtgc gccccggcgt tggaggtcat cacgacgacg gtgttgtcga
    13681 aagagaccgt gcggcccttg ttgtccgtca gccgtccgtc ttcgaggagt tggaggagca
    13741 tgttgaagac gtccgggtgt gctttctcga tctcgtccag caggacgacg gagtacgggc
    13801 ggcgccggac ggcctcggtg agctgcccgc cctcgtcgta gccgacgtag ccggggggcg
    13861 cgccgacgag gcggctggcg gcgtggcgct ccatatactc ggacatgtcg agccgtatca
    13921 tggcgtcctc gtcgtcgaag aggtactcgg caagagcccg cgcaagctcg gtcttcccca
    13981 cgccggtcgg cccgaggaag ataaagctgc ctatcggacg gttcgggttg gcgagaccgg
    14041 cccgcgcctt gcggacggct tcggcgacgg cgaccaccgc cgcgtcctgg ccgacgacgc
    14101 gccggtggag ggcgtcctcc atcgagagca ggcgctccgc ctcgtctttg agcatccggg
    14161 ttacggggat accggtacgg gcgctgacga cgcgcgcgat ctcctccctc ccgacgaccg
    14221 cttcccggac gccagcctcc tccatccagc gctcgcgggc ctcccggaac tcggcctgga
    14281 gggtgtccgc ctccgcgcgc agcagggcgg cctgctcgta gtcacggtcc tcgacggctg
    14341 cggcaccctc gcgggttagc tcctgcaggc gtagctccag ctcccgcaac tcgtcgggca
    14401 tcatggtcgt ctcgatgcgg acctggctcg ccgcctcgtc cagcagatcc accgccttgt
    14461 ccggcaggaa gcggtcggcg atgtagcgat gagatagctg cgcggcggcc ttgagggcct
    14521 cgtcggagat ctcgaccccg tggtgcgcct cgtagcggtc cttgatgccc gcgaggatct
    14581 gaacggtttc ctcttcggtc ggctcctcga tgaggacggg ctgaaagcgg cgctcgaagg
    14641 cggcgtcggt ctccacgtgg cggcggtact cgtctatggt ggtcgcgccg atgacctgca
    14701 cttcgccgcg cgcgagggcg ggcttcagga tctccccggc gtccatcgag ccctcggcgg
    14761 ctccggcgcc aacgaggtta tggaactcgt caacgaagag aatgatcctc ccgtacgcgc
    14821 tcttgacttc atctatgacg cccttcagcc gctcctcgaa ctcgccccgg aacttcgtcc
    14881 cggcaacgag cgagccgagg tccagggaga agatgacccg gtccttcagc acctccggga
    14941 cgtagccggt ggctatcttc tgggccagcc cctcgactat ggcggtcttg ccgacgcccg
    15001 gctcacctat gagggccggg ttgttcttgg tgcggcgagc gaggatctgg atgacgcgct
    15061 ttatctcgtc ctcacgcccc accacgggat ccagcttccc ctcgcgtgcg agccgggtga
    15121 tgtcgcagcc atgcttggcg agcaggctgt ccgggtcttc gcccaatccc ccctcgcccc
    15181 gcatcttccc gaggatgcgg cgcacgtcgg cctcggtcag tccaacttct cgaagaacac
    15241 cggctgctgt ggagccgtca accatcatca gcc
//
LOCUS       DASSMB010000075         3567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_270502, whole genome shotgun sequence.
ACCESSION   DASSMB010000075 DASSMB010000000
VERSION     DASSMB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270502"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="VFJ72_05005"
     CDS             complement(<1..929)
                     /locus_tag="VFJ72_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HET7478858.1"
                     /translation="MEVDRNRLTHAELSENAIAVLKKRYLKKNEKGEAIEEPIDMFRR
                     VSSNIAEGEFRFKEGEEAQTLYEESKENFLQMMLSRKFMPNSPTLMNAGRELQQLSAC
                     FVLPVEDSIDGIYDTLKHQAVIHKSGGGTGFGFSRLRPKNDLVKSTMGVSSGPVSFMA
                     IYDASTDKIKQGGTRRGANMGILRVDHPDIEEFITCKRENDQINNFNISVAITDAFME
                     AVENDTDFYLTNPRDGEVMKTVRARDLFAKIVEGAWLNGEPGVVFIDKINADNPTPQF
                     EIESTNPCSEAHLPPYDSCNLGSINLERFYIEET"
     gene            complement(1052..1585)
                     /gene="nrdR"
                     /locus_tag="VFJ72_05010"
     CDS             complement(1052..1585)
                     /gene="nrdR"
                     /locus_tag="VFJ72_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET7478859.1"
                     /translation="MWCARFLKYQMWWRGYGGYKVQCPYCDFEDTKVIDSRLSETKDA
                     IRRRRECLSPECGKRFTTYERREPLRLMVIKREGGREPFDRDKLRAGLLKACAKQKVS
                     DEDVEVIVDEIEAELRERRRHEVSSRRLGDMALVRLRRLDMVAYLRFASVYRQYTDVD
                     QFRSELVRLAGSGVGVR"
     gene            complement(1881..2324)
                     /locus_tag="VFJ72_05015"
     CDS             complement(1881..2324)
                     /locus_tag="VFJ72_05015"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7478860.1"
                     /translation="MMRRALRAAGEAARKGEVPVGAVVARGDEVIAVAANEREMTQDP
                     TAHAELLALRRAAAALGTWRLTGCTLYATLEPCPMCAGAAHAARISRLVYAAPDPKAG
                     FAGTLHNLPQDTRLNHTYAAEGGLLESESARLLQDFFRDRRQKPR"
     gene            2458..3426
                     /locus_tag="VFJ72_05020"
     CDS             2458..3426
                     /locus_tag="VFJ72_05020"
                     /inference="COORDINATES: protein motif:HMM:NF019346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7478861.1"
                     /translation="MQRSGLTAEGLVGVREVYVRLRENVRGVVVGKDGVVDLALVSLL
                     AGGHILLTDIPGVGKTTLARAIASSIDAEFGRIQFTPDLLPSDITGSSVFDPRQARFE
                     WIAGPVFAPVLLADEINRATPRTQSALLEAMAEGQVTVDGVSRPLPEPFFVVATQNPH
                     QFHGTYPLPEGQLDRFMIAASMGYPERRAELGIVRAQVERRPLEEILPVVGVREVCDA
                     REVVRGVQVHEEVLAYAVAISEATREHPAVSLGVSPRGSIALIRAAQARAATQGRDFV
                     GPEDVKALAPNTLPHRISVRGGATSSSSVTAESVVEEILTSVPPPA"
BASE COUNT          629 a         1083 c         1120 g          735 t
ORIGIN      
        1 gtctcctcta tatagaagcg ctccaggttg atcgaaccga ggttgcacga gtcgtacggc
       61 ggcaggtgcg cctcactgca agggttcgtg ctctcgatct cgaactgcgg cgtcgggttg
      121 tcggcgttga tcttgtctat aaagaccacg cccggctcgc cgttcagcca agcgccctcg
      181 acgatcttgg cgaacaggtc acgcgcccgg acggtcttca tcacctcgcc gtcgcgcggg
      241 ttggtcaggt agaagtccgt gtcattctcg acagcctcca tgaaggcgtc ggtgatggcg
      301 acggagatgt tgaagttgtt gatctggtcg ttctcgcgct tgcaggtgat gaactcctcg
      361 atgtccgggt ggtccacccg gaggatgccc atgttcgccc cgcgccgggt tccgccctgc
      421 ttgatcttgt ccgtagaagc gtcatatata gccatgaacg agaccggccc gctcgaaaca
      481 cccatcgtgc tcttcaccag gtcgttcttc ggacgcagcc gcgagaaccc gaagcccgtc
      541 ccgccgccac tcttatggat gacggcctgg tgcttgaggg tgtcgtagat cccatcaata
      601 gagtcctcga ccggcagcac gaagcacgcc gagagctgct gcaactcgcg gccggcgttc
      661 atcagggtcg gggagttcgg catgaacttc ctcgacagca tcatctggag gaaattctcc
      721 ttcgactcct cgtagagagt ctgggcctcc tcgccctcct tgaaccggaa ctcaccctcg
      781 gcgatgttcg aggaaaccct tcggaacatg tcgatgggct cctctatcgc ctcgcccttc
      841 tcgttcttct tgagatagcg cttcttcaga acggcgatcg cgttctccga caactcggcg
      901 tgggtcagcc tgtttcggtc tacttccata ctcatcctcc ctgaattcta ctgctcgtat
      961 cgcttattcc aacaattact tttattactt ttggtggttt tccccagagg ttacttctgt
     1021 tcttatccgt acccggcggc ttccgccggc cctagcgcac cccgacgccg gagccagcga
     1081 gccggacgag ctcggagcgg aactgatcca catccgtgta ttgccggtag acggaagcga
     1141 agcgcaggta cgcgaccatg tcgagcctcc gcagcctcac cagcgccatg tccccgagac
     1201 gccgcgagct gacctcgtgg cggcgccgct cgcgcagctc ggcctcgatc tcgtccacta
     1261 tgacctctac gtcctcgtcc gagaccttct gtttggcgca ggccttcaac agcccggccc
     1321 gcaacttgtc ccgatcaaac ggctcccggc caccttccct ctttatgacc atcaacctca
     1381 acggctcccg ccgctcgtag gtcgtgaacc gcttcccaca ctccggcgac aggcactccc
     1441 gacgacgccg gatagcgtcc ttggtctcgg atagacggct gtctatcacc ttcgtatcct
     1501 caaaatcaca gtagggacac tgcactttgt aacccccgta gcctcgccac cacatctggt
     1561 acttcagaaa cctagcacac cacattttcg gatcgcaata gcatcttcat aacccttaac
     1621 gtgaggttaa cttgacgtta aactgaataa accgcattta taagcggtct ctgcctccgg
     1681 atagagatcg agaaattttt tcaaaatttt tttcactaat acgcaaaatg taggaattcc
     1741 cagcaaattg cgcaccttta tgttcaggtc aaatgactac attgcaccta tgatttatgg
     1801 ttggcattcc atgcaaaaaa cattcaactg agcatttaag tttaatgtgt atcgacgctt
     1861 gagtcttgtg cgcgtagttc ctatcggggc ttctggcggc gatcccggaa gaaatcttgc
     1921 agaaggcggg cggattcgga ttctagcaga ccgccttctg cggcgtaggt gtggttgagg
     1981 cgggtatctt gcgggaggtt gtggagggtt ccggcaaacc cggctttcgg gtcgggggcg
     2041 gcgtagacga ggcgggagat gcgggcggcg tgggcggccc cggcgcacat gggacacggt
     2101 tcgagggtag cgtagagggt gcagccggtg aggcgccagg tgccgagggc cgcggcagcg
     2161 cggcggaggg cgaggagttc ggcgtgggcg gtagggtcct gggtcatctc gcgttcgttg
     2221 gcggccacgg cgatcacctc gtccccgcgg gcgacgaccg ctcccacggg gacttcgccc
     2281 ttcctggcgg cttcgccggc ggcgcgcaga gcccggcgca tcatggagag gtccgtgctc
     2341 cgctgggggt ctcgttcaag tatttcccgc acgaggccga taatagcagg acggtaacgg
     2401 catgatgcgg ccagagcctg atatacatat ggtgcctatc ttgagcgtgg aggcggagtg
     2461 caacgtagcg gactgacggc ggagggactc gtaggggttc gggaagtcta tgtgaggttg
     2521 cgcgagaacg tgcgtggtgt ggtcgtgggg aaggatggag tggtggatct cgcgctcgtc
     2581 tctctgttgg ccggggggca catcctgctc acggacatcc ccggcgtcgg gaagacgacg
     2641 ctcgcgcggg ccatcgcttc gtcaatagac gcggagttcg ggcggataca gttcacgccg
     2701 gacctcttgc cgtcggacat aaccgggtcg agcgtcttcg acccccggca ggcgaggttt
     2761 gagtggatcg cgggtcccgt cttcgcgccg gtgcttctgg ccgatgagat caaccgcgcc
     2821 acgccccgca cccagagcgc cctcctcgaa gcgatggccg agggccaggt cacggtggat
     2881 ggcgtctcgc gtcctctgcc ggagccgttc ttcgtggtgg ctactcaaaa cccgcaccag
     2941 ttccacggaa cgtatcctct accggagggg cagttggacc ggttcatgat cgcggcgagc
     3001 atgggttatc cggagcggag ggcggagctg gggatcgtgc gcgcccaggt cgagcgccga
     3061 ccgctggagg agatcttgcc ggtcgtcggg gtgcgagaag tgtgcgacgc gagagaggtc
     3121 gtgcgcggcg tgcaggtaca cgaggaggtg ctggcttacg cggtggcaat ctcggaggcg
     3181 acccgcgagc acccggcggt ctctttaggc gtctcgccgc gcgggtccat cgcgctcatc
     3241 cgggcggccc aggcgcgggc cgccacgcag ggacgggact tcgtcgggcc ggaggacgtc
     3301 aaggcgctcg ccccgaacac gctgccgcac aggatcagcg tgcgcggcgg agctacatcc
     3361 tcatcgagcg tgacggcgga gagcgtggtg gaggagatcc tgacatccgt cccgcctccg
     3421 gcttgaggca gcggcgacca ggatgagagc gctgaagcgc atcaggaaga cagccaagtt
     3481 cacgttcctg gcgtgcctcg ccctgatcct gtacgccatc gccagcacct ccggcgcggg
     3541 gtgggtgtac gtgatctcgg cgggcat
//
LOCUS       DASSMB010000076         8397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1411487, whole genome shotgun sequence.
ACCESSION   DASSMB010000076 DASSMB010000000
VERSION     DASSMB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8397
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411487"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFJ72_05025"
     CDS             complement(<1..227)
                     /locus_tag="VFJ72_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478862.1"
                     /translation="MMDGNNQRPLMYVATHGTDDPTLATMPFLIATGAGTAEIDCCIA
                     LLGEAAILAKPGMIDAVHGVGFPPLRELVQKV"
     gene            complement(228..479)
                     /locus_tag="VFJ72_05030"
     CDS             complement(228..479)
                     /locus_tag="VFJ72_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HET7478863.1"
                     /translation="MASGVESEQRVLLDLRGSVCPGPTGDTLAALKKLPPGGGLAVIT
                     DYPPARQTIPRLVEKRGCRWEITEDDGTVFRLEITKGAE"
     gene            complement(666..1643)
                     /locus_tag="VFJ72_05035"
     CDS             complement(666..1643)
                     /locus_tag="VFJ72_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7478864.1"
                     /translation="MAQNNDKSGGGDGGTKKVVYAAFAANLLITISKFIAGFITGSAS
                     LLAEGAHSIADTVNQVFLLVSLRSAKSKPDRTHPYGYGKDRFFWSLIVAVGLFVAGAV
                     FSFYEGISKILEGSGGESASFLAGYIVLGVAFVFEGASFVITTREFGKSAREEERTFR
                     EHFRMTRNTTMKVPLYEDSAALVGLLIAFAGLLFSQLSGSHVYDGIASIAIGVVLVFV
                     AYELGRDSRALLLGEAVPPEDEERLRETITGFEEVNDIVRLLTMHLGPESVLVNAEIH
                     VADGMDTDGIEDLIARITKRIGEQLPEVTQTFLELHPPQEEGQTAPKSD"
     gene            complement(1731..2120)
                     /locus_tag="VFJ72_05040"
     CDS             complement(1731..2120)
                     /locus_tag="VFJ72_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478865.1"
                     /translation="MRWEEYMVKGMAAALVITGLFSLWMLWQLADVRLPSLTPSAIAQ
                     DTTSTGLETTAETTSESTAFQETTQQGATQATTVEETTTQETTVQRPNKLKNSGGPRS
                     RAVPLMPGGRCPKEFPVKRQGTCHARR"
     gene            complement(2243..2971)
                     /locus_tag="VFJ72_05045"
     CDS             complement(2243..2971)
                     /locus_tag="VFJ72_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7478866.1"
                     /translation="MINRSGDTYTVDEASALLGVPRPTLYRYLKEYSIPYQRRSGRIS
                     VPEESLERIRTVRELHDEGLGTAAVRRRLQAGEGSDVDWIAERLDRLSEALETSQSSL
                     KPTEGVSSSQALHIILARQSLLISAVFNLTEMMEELLAVSGRPRKPALDYLDEQSRLE
                     ADASLPPDLRDDALALRGVSDVSPGVRAPAEPEPIEAVPVHGRRDGFGALARRRRRTV
                     AALAFLVILGVVAAGVFFWRAAGL"
     gene            3232..3879
                     /locus_tag="VFJ72_05050"
     CDS             3232..3879
                     /locus_tag="VFJ72_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7478867.1"
                     /translation="MNEGGYVLHRLFSVLWIFALAFAVMAFVNVDRANAETALASWYG
                     PGFEGLPTASGEPYDPSGYTAASKTLPLGTELIVSYQGNSVPVTINDRGPYVGSRDLD
                     LSQAAAQTLGLDQAGVDYVDYQYADSTSYAPTQTTSQATYTEPAPEPAATEPAPTSSG
                     YETTSMPQSGGAYVVQPGDTLSEIALQLGTTTEYLASNNGVADPDYIQSGQTIYY"
     gene            3985..4743
                     /locus_tag="VFJ72_05055"
     CDS             3985..4743
                     /locus_tag="VFJ72_05055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7478868.1"
                     /translation="MSEGGMERLRGKAALVTGASSGLGKATAIALAAAGADVGLVARS
                     EKDLRGVEAEISGAGGRALPLPTDLASAEQTFAAVEAAVGEFGRLDILVNAAGTDAPG
                     AVADLDVEGWDRTLDVNLRAPYLLSGAAFPHMREAGSGLILNVSSVAGKKGWANAAAY
                     CASKFGLTGLTESLADEGREHGIRACIIYPGAMATNWGVWNPEDRKDESGEEAASGAL
                     PPSQVADFIVWLAAAPPGFVLTEGIIVPVEEGLP"
     gene            4845..5129
                     /locus_tag="VFJ72_05060"
     CDS             4845..5129
                     /locus_tag="VFJ72_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478869.1"
                     /translation="MDEQGSGKKPEEEPAGWHTQAPRVSDAFRKGVSVTVTTWGGRSV
                     EGLVCDRDPAGVLLETRVGSNPGYVFLPWSSVEQVAIDQLTPRRVKFLQS"
     gene            5243..5752
                     /locus_tag="VFJ72_05065"
     CDS             5243..5752
                     /locus_tag="VFJ72_05065"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HET7478870.1"
                     /translation="MAELVDRPESGEDQSTGEDRQERTARELIELLQELRVVLPGVQI
                     LFAFLLTVPFSQRFASTTPLQQNVFFATLFCTAVATVLLIAPSAHHRMLWRRHLREER
                     LYAANRLTIGGLAFLVPAMAGAIFVVSDLTFGSTAGSATAAVVFLLAVYLWFVHPLVA
                     FPSGKDGEN"
     gene            5920..7032
                     /locus_tag="VFJ72_05070"
     CDS             5920..7032
                     /locus_tag="VFJ72_05070"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HET7478871.1"
                     /translation="MSWGRKISPLANALEERYDAARFGVKRRFGLLDPFEILPYRGHG
                     TRRELFLRGRVLEERGITRSGQSSSLLTNLGNMARRFASDEVPSARVRASFAGIEVDT
                     VADEEGFFDVRFELPEPLPESNAWHPVELELLSPPSPGGGVVRSTGQALVPEGARFGV
                     ISDLDDTVVRSNATNALKMAWITLLNDARTRLPFAGVSAFYQALRRGPSGRDDNPTFY
                     VSSSPWNIYDVIEDFLNVHDVPPGPIFLKDWSLTALGKHHEHKLGLIRGLLRTYPEMP
                     FVLVGDSGQEDPEIYVQTVREHPGRILAIYIRDVTAADRDAQVKSLMKEASDLGVEMV
                     LTEDTAVAAEHAASAGLISPGAVSEVRSSARGAARG"
     gene            complement(7039..>8397)
                     /locus_tag="VFJ72_05075"
     CDS             complement(7039..>8397)
                     /locus_tag="VFJ72_05075"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7478872.1"
                     /translation="RGQMFDAVVTIVGCDKTIPAAAMAHVRLGIPGVIVYSGSIKPGR
                     FRGRDVVVGDVFEGVGANAAGKMSDADLKELEEHACPGAGACGGQYTANTMAMILEFL
                     GLSPMGSANPPALDGRKDEVCVEAGRLVMKLLERDLTPEKILTRESFENAIAAGAATG
                     GSTNLVLHLLAIAREAGIPLDIDDFDRVSDRTPIIADLLPGGRYTAVDVDAAGGSKLI
                     GKRLLDAGLIDGSQMTTTGRTLAEEVEDAEETEGQDVIVPVENPLRETGGLVILKGNL
                     APEGCVVKVAGYTRLTHTGPARVFDSEEEAMEAVQAGGISGEDVVVIRYEGPKGGPGM
                     REMLGVTAALVGQGLGETVALLTDGRFSGATRGLMAGHVAPEAAHRGPIAALRYGDTV
                     TFDITNRTLSVDLTDAEIEDRLSNWTEPEPRFTQGVMAKYAALVSSASKGAITSPENL
                     GK"
BASE COUNT         1364 a         2769 c         2808 g         1456 t
ORIGIN      
        1 accttctgga cgagctcgcg gagcggcggg aagccgacac cgtggacggc gtctatcatg
       61 ccgggcttcg ccaggatcgc cgcttctccg agcagggcta tgcagcagtc tatctccgcg
      121 gtcccggctc cggtggcgat caggaagggc atggtcgcca gggtggggtc gtcggtgccg
      181 tgcgtcgcga cgtacatcag cggtcgctgg ttatttccgt ccatcactca ctctgctcct
      241 ttggttatct ccagccggaa aaccgtcccg tcgtcctccg tgatctccca gcggcatccc
      301 cgcttctcga ccagccgggg gatggtctgc cgggcgggag ggtagtccgt gatcaccgcc
      361 aggccgcccc ccggcgggag cttcttgagg gccgcgagcg tgtcgccggt cggccccgga
      421 catacgctgc cccgaaggtc cagcaggacc ctctgctcgc tctcaactcc gctcgccatc
      481 gcgctcctta tcctcgtcct tctctttccc aactacccat tgtactttcg ggcacgatcc
      541 ggcacatccc ccaaataggg gatttaatct ttacgagaga cagtccaggg agggggccac
      601 ggtgcggttc cggggcccgg tggaccgcct gatccgcaag acctgagcgc tccgctccct
      661 tactctcagt cgctcttggg cgcggtctga ccttcctcct gcggcgggtg cagttcgagg
      721 aaagtctggg tcacctcggg tagctgctcg ccgatcctct tggttatgcg ggcgatgagg
      781 tcttcgatgc cgtcggtatc catcccgtcg gcgacgtgga tctcggcgtt caccaggacg
      841 gattccggtc ccaggtgcat cgtcaatagc cgcacgatgt cgtttacctc ctcaaagccg
      901 gtgatcgtct cccgcagacg ctcctcgtct tcgggcggca cggcctcgcc gagcaacagg
      961 gcgcgggaat ccctgccaag ctcgtaggcc acgaagacca ggaccacccc gatggcgata
     1021 gaggcgatac cgtcgtagac gtggctcccg ctcaactggc tgaacaacaa ccccgcgaac
     1081 gcgatgagga ggcccacgag cgccgccgag tcctcgtaga gcggcacctt catcgtcgtg
     1141 ttgcgggtca tcctgaagtg ctcccggaac gtccgctcct cctcccgcgc ggacttcccg
     1201 aactcccgcg tcgttatcac gaaagacgcg ccctcgaaga cgaacgcgac ccccaggact
     1261 atgtagcccg ccaggaacga cgcgctctct ccaccggagc cttcgaggat cttagagatt
     1321 ccctcgtaga atgagaagac cgcgccggcg acgaagagac cgaccgcgac gatcagggac
     1381 cagaagaatc ggtccttccc gtacccgtag ggatgggtcc ggtcgggctt gctcttggcc
     1441 gaacgcaaac tgacgagcag gaagacctga ttgacggtat cggctatcga gtgcgctccc
     1501 tcggccagaa ggctggcgct gcccgtgatg aacccggcga taaacttgga gatggtgatc
     1561 aggaggttcg ccgcgaacgc cgcgtagacg actttcttgg tcccgccgtc cccgccgccc
     1621 gacttatcgt tgttctgcgc catttgctcg ctcccggata aagaggtccc aggagaccag
     1681 ttatccctaa atgtcctccg catgaaaccg cgaagcacta cctcctggcc ttacctcctg
     1741 gcgtggcagg ttccctgacg cttcaccggg aactctttcg ggcaccggcc gccgggcatg
     1801 aggggcacgg cgcgggagcg ggggccgccg gagttcttga gcttgttcgg gcgctggacc
     1861 gtggtctcct gcgtggtggt ctcctcgaca gtggttgcct gggtcgcgcc ctgctgggtg
     1921 gtctcctgga acgcggtgct ttcgctcgtg gtctctgctg tcgtctccag gccggtgctg
     1981 gtggtgtcct gggcgatggc ggatggcgtg aggctcggga ggcgcacgtc ggcgagctgc
     2041 cacagcatcc acaggctgaa gagccccgtg atgaccagcg ccgcggccat ccctttcacc
     2101 atgtactctt cccaacgcat aaagcgctac ctcccccgca tatgagatct gaacatgtat
     2161 tctaaccgcg agggcacgtt tgagccagca atcgggtctt ctccgggcgg tgggcgcggt
     2221 gggggaggta gcgccggtac ttttagagac cggctgcgcg ccagaagaac acgccggccg
     2281 cgacgacccc gaggataacc aggaacgcga gagccgccac ggtgcgccgc cggcggcggg
     2341 cgagagcgcc gaacccgtca cggcggccgt gaacaggcac ggcctcgatg ggttccggct
     2401 ccgcaggtgc gcggacgccg gggctcacgt cggagacacc gcggagcgcg agggcgtcgt
     2461 cccgcaggtc cgggggcagg ctggcgtccg cttccaggcg gctctgctcg tccaggtagt
     2521 cgagggcggg cttgcggggc cggccggaga cggcgagaag ctcctccatc atctccgtca
     2581 ggttgaagac ggcggagatc aggaggctct gccgcgccag gatgatgtgg agcgcctgcg
     2641 aggacgagac gccctcggtc ggcttgaggc tcgactgcga ggtctccagg gcctcggaga
     2701 gccggtccag ccgctcggcg atccagtcaa cgtccgagcc ctctcccgcc tgcaaccggc
     2761 gccgcacggc cgcggtcccg agcccctcat cgtgcaactc gcgcaccgtc cgtatccgct
     2821 ccagagactc ctcggggacg gagatcctgc cggacctccg ctggtagggg atggaatatt
     2881 ccttgaggta gcggtacagc gtgggccgcg gcacaccaag cagcgcgctg gcctcatcca
     2941 ccgtatatgt gtccccgcta cggtttatca tggcaatatt ctacagaatt tttcgagccc
     3001 ttcgtagcga ggtctctgcc acccctcgtc tcgcgccatg ataatacccc cgataaaccg
     3061 catagataag cggcgcgcag tctcttgaca agagaaaatt cggaggtgtc cggcaggccg
     3121 cggggaaggg agatgaccac cgcgagataa cggctagaac gctcttgatc ctctggatga
     3181 ggtcgtcata ctgaccccgt aatcgaagtt catttgaacc ggaagtagac gttgaacgag
     3241 gggggatacg tgctacacag gctctttagc gtgctgtgga tctttgctct ggctttcgcg
     3301 gtcatggcat ttgttaatgt ggaccgggcc aacgccgaga cggcgctcgc gtcgtggtac
     3361 ggtcctgggt tcgaggggct accgacggcc agcggcgagc cctatgaccc gtcggggtac
     3421 acggccgcgt ccaagacgct gccgctcggg acggagttga tcgtcagcta tcagggcaac
     3481 tcggtgcccg ttacgatcaa cgaccgcgga ccctatgtgg ggagccggga cctcgacctc
     3541 tcccaggcag ccgcgcagac gctgggcctc gaccaggccg gtgtagacta cgtggattac
     3601 cagtacgctg acagcacttc ctacgcgccg acgcagacca catcacaggc tacgtatacg
     3661 gaacccgcgc ccgagccggc tgccacggaa ccagcgccga ccagcagcgg gtacgagacg
     3721 acttcgatgc cccagtccgg cggagcctac gtcgtccaac caggagacac gctctcggag
     3781 atagccctgc agctcgggac caccacggag tacctggcga gtaacaacgg agtcgccgac
     3841 ccggactaca tccagagcgg gcagacgatc tactactagc ccggcccctt gtagacgctg
     3901 atcatccggc gcgggctggc gagacccggc atactgcggg tccgggcggt ggagcgcgga
     3961 tgatattcgg aagggacttc tgtgatgagc gagggtggaa tggaaaggtt gcgcggcaag
     4021 gccgcgctgg tgacgggcgc gagcagcggt ctcgggaagg ccacggccat cgctctcgcc
     4081 gcggccggag cggatgtcgg gctggtggct cgcagtgaga aggacctgcg gggcgtggag
     4141 gcggagatct cgggcgccgg aggacgggcg ctcccgctgc cgaccgacct cgccagcgcc
     4201 gagcagacct tcgcggcggt agaggccgcg gtcggggagt tcgggaggct ggacatcctg
     4261 gtcaacgccg ccgggacgga cgcgcccggc gcggtcgcgg acctggacgt ggagggatgg
     4321 gaccggaccc tcgacgtcaa cctgcgggcg ccgtacctgc tctcgggggc cgcatttcct
     4381 cacatgcggg aggcggggtc cggcctgatc ctgaacgtct cctccgtggc ggggaagaag
     4441 ggctgggcga acgccgccgc ctactgcgcc tcgaagttcg gtctgacggg ccttaccgaa
     4501 tctttggccg atgagggccg ggagcacggc atccgggcgt gcatcatcta ccccggcgcg
     4561 atggcgacga actggggggt gtggaacccc gaggaccgga aggacgagag cggcgaggag
     4621 gccgcgtccg gggctcttcc gccctcccag gtcgcagact tcatcgtgtg gctcgcggct
     4681 gccccgccag ggttcgtcct gaccgagggc atcatcgtcc cggtcgagga gggcctgccc
     4741 tgagttgccg cacccgtgac gactattgtc aggatccgcc tcatttccgt aggacatgat
     4801 gtataaagag atcagccgcc ggggtcgaga cggcgtgagg catcgtggac gagcaaggat
     4861 caggcaagaa gccagaagag gagccggcag ggtggcacac ccaggctcca agagtctctg
     4921 acgctttcag gaaaggcgtc tccgtaacgg ttacgacgtg gggcgggcgc tccgtcgagg
     4981 gactggtctg cgaccgggac ccggcgggag tcctgttgga gacgcgggtg ggaagcaacc
     5041 ctggatatgt attcctcccg tggtctagcg tcgagcaggt agcgatagat caactgacgc
     5101 cccggcgcgt caagttcctg cagagctaac ggatgggagc gtcgcggctt ctatgcctgc
     5161 cagaaggcgc cgccgagcct atccggtaat ccgcggctcg cgtataatac aggttaaatc
     5221 tgtgttaaac ggaggcgttg acatggcgga gttggtagac cggccggaga gcggcgaaga
     5281 tcagtcaacc ggggaagacc ggcaggaacg cacggcgcgg gagctgatcg agcttctgca
     5341 agagctccgg gtggtgcttc cgggggtcca gatcctcttc gctttccttt tgacggtgcc
     5401 gttctcgcaa cggttcgcca gtaccactcc gctgcaacaa aacgtcttct tcgcgacgct
     5461 attttgtacg gcggtcgcga ccgtgctcct catcgcgccg tccgcccatc accgcatgct
     5521 gtggcgcagg cacttgcgcg aggaacgtct gtacgcggcg aaccggctga ccatcggggg
     5581 gctcgctttc ctcgtgccgg cgatggcggg ggctatcttc gtcgtctccg acctcacgtt
     5641 cggttccacc gccggctccg caacggctgc cgtggtcttc ctactcgccg tctacttgtg
     5701 gttcgtgcat ccgctcgtgg ccttcccatc cgggaaggac ggcgaaaact agcaagagcc
     5761 gtcctctccg gcattacttt gcggtcaggc ggtattctca cccgccgcag gagatatacg
     5821 agcgctccat cggcgagtct ggctgcttgc gacaggttcc gggatgcagg gtaggggtag
     5881 agtatctcgc cggaaactcg gaaacagagg ggagaagaga tgagttgggg aaggaagatc
     5941 tcacctttgg ctaacgcgct ggaggaacgg tacgacgcgg cgcggttcgg ggtgaagcgc
     6001 cgcttcggct tgctggaccc gttcgagata ctcccgtacc gggggcacgg cacgcggcgg
     6061 gagctgttcc tgcgcggccg cgttctggag gagaggggca tcacccgctc cggccagagt
     6121 tcctctctcc tgacgaacct cggcaacatg gcccgccggt tcgccagcga cgaggtcccc
     6181 agcgcgaggg tgcgagcctc attcgcgggg atagaggttg ataccgtggc cgacgaggag
     6241 gggttcttcg acgtgcggtt cgagctgccg gagccgctgc ccgagtctaa cgcctggcat
     6301 ccggtagagc tcgagttgct ctcgccgcct tcgcccggcg ggggcgttgt gcgttccacg
     6361 gggcaggcgc tcgtcccgga gggggcgcgc ttcggcgtga tcagcgacct cgacgatacg
     6421 gtggtgcgct ccaacgcgac caacgcgctg aagatggcct ggatcacgct gctcaacgac
     6481 gcccggacgc ggctgccttt cgcgggcgtc tcggccttct accaggcgct gcggcgcggc
     6541 ccgtccggcc gggacgacaa ccccacgttc tacgtctcca gcagcccgtg gaacatctac
     6601 gacgtgatcg aagacttcct caacgtccac gacgtgccgc ccggtccgat cttcctgaag
     6661 gactggagcc ttacggcgct cgggaagcac cacgagcaca agctcggcct catccgcggc
     6721 ctgctccgca cctacccgga gatgccgttc gtgctcgtcg gggactcggg acaggaggac
     6781 ccggagatct acgtccagac cgtgcgcgag caccccggac gtatcctcgc catctacatc
     6841 cgggacgtta ccgccgccga ccgggacgcc caggtcaaga gcctcatgaa ggaggcgagc
     6901 gacctcggcg tggagatggt gctcacggag gacacggcgg tggccgccga gcacgccgcc
     6961 tccgccggtc tcatctcgcc cggcgcggtc tccgaggtcc gcagctccgc gcgcggggct
     7021 gctcgcggct gaggcgcgct acttcccgag gttctccggg ctggtaatgg ctcccttcga
     7081 ggcggaggag acgagcgcgg cgtacttcgc catcacgccc tgcgtgaagc gcggctccgg
     7141 ctcggtccag ttggagaggc ggtcttcgat ctccgcgtcc gtgaggtcca cgctcagggt
     7201 ccggtttgtg atgtcgaagg tcacggtgtc gccgtagcgc agggcggcga tcgggccgcg
     7261 atgggccgcc tcgggggcga cgtggcccgc catcaggccg cgggtcgcgc cggagaagcg
     7321 cccgtccgta agcagggcga ccgtctctcc caggccctgg ccgacgagcg cggcggtgac
     7381 gcccagcatc tcgcgcatgc ccggaccgcc cttcggtccc tcgtaccgga tcacgaccac
     7441 gtcctcgccg gagatgccgc ccgcctgcac ggcctccatc gcctcctcct ccgagtcgaa
     7501 gacgcgggcc gggcccgtgt gcgtgaggcg cgtgtagccc gcgaccttca cgacacaccc
     7561 ctccggcgcg aggttgccct tcaggatcac gagcccgccg gtctcgcgca gcggattctc
     7621 gaccgggact atgacgtcct gcccctccgt ctcctcggcg tcctctactt cctcggcgag
     7681 tgtccggccg gtggtggtca tctgcgagcc gtcgatcagg cccgcgtcga gcaggcgctt
     7741 gccgatgagc ttcgatcccc cggccgcgtc cacgtccacg gcggtgtatc gcccgccggg
     7801 cagcaggtcc gcgatgatcg gggtgcggtc gctgacccgg tcgaagtcgt ctatgtcgag
     7861 cgggatgccg gcctcgcgcg cgatggcgag gaggtgcagc accaggttcg tcgagccgcc
     7921 ggtggccgct ccggccgcga tcgcgttctc gaacgactcg cgggtgagga ttttctccgg
     7981 cgtgaggtcg cgctccagca gcttcatcac caatctgccg gcctcgacgc agacctcgtc
     8041 cttgcggccg tcgagtgcgg gcgggttggc ggagcccatc ggggagaggc cgaggaactc
     8101 aaggatcatc gccatcgtgt tcgccgtgta ctggcctccg caagcccccg cgccggggca
     8161 ggcgtgctcc tccagctcct tcaggtccgc gtcgctcatc ttgcccgcgg cgttcgctcc
     8221 gacgccctcg aagacgtcgc cgaccaccac gtcgcgcccc ctgaaccgcc ccggcttgat
     8281 cgatcccgag tacacgatga cgccaggaat cccgagccgc acgtgcgcca tcgccgccgc
     8341 cggtatcgtc ttgtcgcagc cgacgatggt caccacggcg tcgaacatct gcccgcg
//
LOCUS       DASSMB010000077        20882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1156527, whole genome shotgun sequence.
ACCESSION   DASSMB010000077 DASSMB010000000
VERSION     DASSMB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 20882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20882
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156527"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /gene="hemB"
                     /locus_tag="VFJ72_05080"
     CDS             <1..547
                     /gene="hemB"
                     /locus_tag="VFJ72_05080"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET7478873.1"
                     /translation="LELLADVALSHARAGADVVAPSDMMDGRIGAIRAQLDAEHCEHV
                     SIMSYSAKYASAYYGPFREAAGSTPQFGDRRAYQMDPANAREALVETVLDIEEGADIV
                     MVKPALPYLDVLRRVREMTNLPVAAYNVSGEYAMMKAAVQNGWLDEERAVMEALTGIK
                     RAGADIIISYHAPDVASWLGE"
     gene            565..1710
                     /locus_tag="VFJ72_05085"
     CDS             565..1710
                     /locus_tag="VFJ72_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478874.1"
                     /translation="MTRKVMVWREDGSRPVLTEVLADDEFQLQEIVKDTPDLLPVDEF
                     GMTGPLMIVGRETTLPSGSVDLIGVSRDGELLLIEFKTGPQNSDFRHVLAQLLDYGSD
                     LWRMSYEEFESTVARRFFSGDHCRDARLRGKASIIDAALEIWPDLSDEETASLRERLS
                     QQLESGEFHYVVVAQRFRDTMERTVEYLNSTMRGARFYAVELVRFSAGNLSAFESRTV
                     LKPESHGSGSTQRTRTSETSRTRTNEIQFLERVEDDAYRETLRDLLDVCHGLGLQFSW
                     GPAGTSIRMTSLDRPRPLSIAWLFPPGVSGWMGLTDLTLGHSENQEDVPQAVQNYVDR
                     VSKLPGVEVAKPKWLSGYRLGPETAIQNHHQIVEILAELVSQSGEET"
     gene            1828..3108
                     /gene="hemL"
                     /locus_tag="VFJ72_05090"
     CDS             1828..3108
                     /gene="hemL"
                     /locus_tag="VFJ72_05090"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HET7478875.1"
                     /translation="MDRSARLMERAVQRMPGGVNSPVRAFRSVGGDPLFMERGEGSRI
                     FDVDGNSCVDYVMSYGPLVMGHAHPETIEAIERTARKGTTFGAPTELEVELAELVCES
                     VPSVEMVRMTNSGTEATMSAIRLARGYTGREKILKFDGNYHGHGDALLVAAGSGVATL
                     GLPDSPGVTKGAAKDTAVLPYNDLEAVRELFEREGEEFAAAILEPVAGNMGCVPPAEG
                     YLEGLRELTAEYGVVLIFDEVMTGFRLARGGAQERFGVTPDLTTLGKIIGGGLPVGAY
                     GGKREIMDLIAPSGPVYQAGTLSGNPLAMAAGLTTLRATAEPDFYERLEQSGERWRQG
                     MNEAASSGGVPFTINQVGSMVSIFFTGGPVTDFETAARSDIEAFKDFFWHMLSRGIYL
                     APSQYEAGFISTAHTEEDLNRTFEAAGEWFAGRG"
     gene            3109..3543
                     /locus_tag="VFJ72_05095"
     CDS             3109..3543
                     /locus_tag="VFJ72_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478876.1"
                     /translation="MERAARRTPEVSLEEVFRRASRGVPDEEERAAVVAEALGYLQRG
                     FDSHYASGERATDDEILVGDNAYAWAVETISRLDEPEFVAVASRMIRDGAGRVSAGGG
                     VDLELWTPHLADLLRIISGEDPERSRERITAAAGEVAGERSA"
     gene            3527..4270
                     /locus_tag="VFJ72_05100"
     CDS             3527..4270
                     /locus_tag="VFJ72_05100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7478877.1"
                     /translation="MNGARKGPSPAGKLPSGTERARLVQEMFDRIAGRYELLNTVMTA
                     GLNRIWNRKVLAATGLRPGDRALDLACGTGSLTRDLARRVGPEGSVLGVDFSREMLRA
                     AERRPLPNIEYQLGDATRLDGVPSEAFDAATIAYGARNIPDLDALFAEMARCVRPGGA
                     VVCLEIARPAGRIASAFYGLWFDKVVPRLGARISGDAWAYSYLPESVKEFVAPDELAV
                     IMRRNGLQSVTWSRLAGGIITLHRGFRRA"
     gene            4194..5252
                     /locus_tag="VFJ72_05105"
     CDS             4194..5252
                     /locus_tag="VFJ72_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7478878.1"
                     /translation="MDYKALHGAGSRAVSSPSTVDSGVHSFEQSASDLTRALPDAAFE
                     VWQEVEDRLVVVGERAPAALAQPALESLTSGGKRLRPLLLILSARLGEPNRESLLRAS
                     TAIEVLHTATLIHDDIVDRAESRRGQPTTVAAYGREIAIATGDYLFAESFSELAGIGD
                     PRLVRAFSEAAEGLAAGELEQYRASGAPVELEAYLDHIRKKTAGLFKAACVAGGTLGG
                     LSLRQIDALATYGNALGIAFQMSDDVMDLVGKPGLMGKGIGTDLAEGTVTLPVIFALQ
                     EGDAEKIREVLADPHPSPDLLEAGIEAVLATDAIEKTEAWARGEIDAAVDDLSILPDT
                     AERAILEAVAAEVVGRDA"
     gene            5245..6486
                     /locus_tag="VFJ72_05110"
     CDS             5245..6486
                     /locus_tag="VFJ72_05110"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7478879.1"
                     /translation="MPEKSVIFAADTVFPVSAPAVRNGAALVAPDGRIGAVGTLEDLR
                     RAEPDAEVREFPGCVILPGAVNAHAHLGFRRKDAPEGGTFAGWLGRLIEKLPEKEAWT
                     AEAARDSAREAIEAGTTFMAESSPYGECLPQLAESGMAGAVYAEFFPGDFDGAGSEEI
                     VDSIIGKVKDLGEGLPERVAARVSVHSPYTVDPESSRLAAHRTREMGWRLAIHLSESP
                     EEVEFVRDGTGGLADIFGKNEWGGRGVSPVRYAEEIDLLAPLTIAAHLATGVSDDDIE
                     VLSRSGVAAAHCPRSNDFLGCEVSRVSEMMRHGVRVGMGTDGLWSSPSMNLFEETLFA
                     VRLHGFDGATGLRLASLEGARALGIEHETGSIEAGKWADLAVVEAGPGGNDPERDVLE
                     SAAGGGVAATVAGGNLIYNRV"
     gene            6516..7160
                     /locus_tag="VFJ72_05115"
     CDS             6516..7160
                     /locus_tag="VFJ72_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7478880.1"
                     /translation="MMMNRDRLGFWTKVFAIGLAAVFLLSFIFMGVGTNINYNLFDLF
                     GGNKNAKQGGQTVSAQEQIRQAEKQVEKNPKDPQAVQTLAALYYQNNRPDDATKVLEK
                     GRKDMPKNADIANTLGSVYAQQAQAASDKEKEKLFAKAGDSFADATKLEPKSADSYYF
                     AGQAYDQAGDSAKAIEYYNGYLDRAPKGDQAKAVKDRISQILKGENTTGASGGG"
     gene            7238..7540
                     /gene="tatA"
                     /locus_tag="VFJ72_05120"
     CDS             7238..7540
                     /gene="tatA"
                     /locus_tag="VFJ72_05120"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7478881.1"
                     /translation="MFGSLGPTELIIALVIILLLFGAKKVPELARGLGSGVKEFRAGT
                     REGEVKDKEKEKDEELAAAESKTDKTGEAHDPHLEEDAERPRDVTAEADRAEQKQR"
     gene            7566..8402
                     /gene="tatC"
                     /locus_tag="VFJ72_05125"
     CDS             7566..8402
                     /gene="tatC"
                     /locus_tag="VFJ72_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7478882.1"
                     /translation="MAGRLRLPTRSREARGEARMTLIEHLDEFRSRLIKVAIAFVAVS
                     IVAWFFRDRIFDALLAPAGDALHGKLNVTGVTEQLFTDMKLALYVGFLLTIPILLYQA
                     WAFVAPAVGDLGRAFTYTIITLASVLFLAGVAFGYFLVLPIGTQFLLSWDPDRYDTII
                     TSQSYLPFVTRFLLAFGIVFELPAATYVAAKLELVDAPFLKKYRRHAIVLNTILAAAL
                     TPGQDPYSMILMAVPMIVMYEASILIARYVNPVSEVRVRELGEEDDADHDEIEEPEER
                     DL"
     gene            complement(8421..9329)
                     /gene="menA"
                     /locus_tag="VFJ72_05130"
     CDS             complement(8421..9329)
                     /gene="menA"
                     /locus_tag="VFJ72_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HET7478883.1"
                     /translation="MAAGRGRSVLAEWFWLARPFSLTAAALPVLFGSALALADGAFRG
                     GAFLTMLSGSLLIQAATNMFNEFYDEKRGLDTAGAVGIAGSIVQGRMHARSVLIGALF
                     CYTLALFCGIYLIAVGGWPILVLGCLSALGGYLYSAGPRPIAYTAASEAAVFIFMGIL
                     IVVITYGVQAGEFPLYVPFAALPFGGPVAAILLANNIRDMNSDRRGGRRTLPIVLGRR
                     RAILIYRLLLLEAYVALIALILLGIVPAECAMALVSLPMVAKLWRNITSSTVPARLDP
                     VVKATAGFHAVFGLLYTLGVLAGGLA"
     gene            9486..10376
                     /locus_tag="VFJ72_05135"
     CDS             9486..10376
                     /locus_tag="VFJ72_05135"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HET7478884.1"
                     /translation="MEAGGTTGFAGSFLRLARPKQWTKNGFVMAGLVFSGKALSASPV
                     LDAVLAFAAFCLLSSAVYAANDVLDIEEDRKHPLKRLRPVASGEISARSALTFAFLLA
                     AGGLAVAFFGVNPGVGIAGLAYIGLQAVYTTLLKHMAILDVMSISAGFVIRALAGVVA
                     VGSPVSPWLIVCTGLLTLFLGFSKRRHELATLGERAAIHRRNLRDYSVPMLDEMMNIM
                     LSATIIGYALYTFTVYRSEQEIFMMSSIPFVIYGVFRYMLLVHRDGGGNPDMLLLRDR
                     PLQVSLLLWLAVVMTVIYIL"
     gene            10381..11358
                     /locus_tag="VFJ72_05140"
     CDS             10381..11358
                     /locus_tag="VFJ72_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7478885.1"
                     /translation="MQRIGRNLTLALGLGVAVYLVLAVFSGFDDLRDALENFRWSLAP
                     AILGLVAVSYTGRFLRWRYYLRLLKVSVPAPVNAAIFAAGLSMAISPGKFGEVLKSVF
                     VRQVSGAPIARTAPAVVAERATDGTGMVAWGLLGALAFSFGPWVLILFLAVTVAGIAV
                     LRSRRLSLLAERILSRLPLLNRLAPHLEDFHGASNVLLGTRPLIVGTAISFLSWGLEI
                     LAVYLCSVGIGAGLPFLMVVFVFAVGSLIGVGSMLPGGIGAAEASMAGMFKFYAGLAA
                     GPAVALTLLIRLATLWFATLIGIAGLLIVRGVIGDIVPDDVATQPGTER"
     gene            11436..13544
                     /locus_tag="VFJ72_05145"
     CDS             11436..13544
                     /locus_tag="VFJ72_05145"
                     /inference="COORDINATES: protein motif:HMM:NF005555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HET7478886.1"
                     /translation="MRLIISEKANAAKKIAQFLAEGSVKEGKYRSVPHHTFTWKGEEC
                     VSVGLKGHVLNPEYPEEYSNWQKVEPSKLIDAEILKSVSEKGVAEAIKSLSKKADRVV
                     VATDFDREGELIGVEALSLVFEANPKLMDHVERSRFSALTPGEVNRAFDNLVEVSREL
                     AAAGEARQDIDLIWGATLTRWVSRATKRYGSAFLSVGRVQSPTLALIAARERERRAFV
                     PEPYWELEATLKNGEPFTVRHATERFKEEAAVRAAYENLAETATVTEVKQKSASRQPP
                     APFNTTSFLTAAASIGISPSRAARLAEDLYTDGYISYPRTDNTVYPRSLDLREVLGYL
                     KRVEGAGQHAERLLAQEKLSPTRGKKETTDHPPIYPTGYASKKALRDDQWKIYQLVVR
                     RFLATLMGPAKTLRTTLRFDSGGEPLTTGGTVVTEEGWLAAYPYSRRADEEIPNLSEG
                     EVVKVVSSEILAKETQPPGRYGAGRLINVMEDLGLGTKATRPNIIQNLYDRGYVHDDP
                     LIPTEKGIKVAQALTDFASEIASHEMTAELERSMDAISEGKISKENVVDESRDVLRRV
                     YEHLQNSETEFADIVWEGIRTDETLGKCPESGHDLIIRRNRKSRKRFVGCSGYPECKV
                     TYPLPQRGEIIPLGTQCDACGSPEIKVLGGKRPWITCINMDCPKKQEQKEAAAKADED
                     RQEVEQPSTERAQELEKSTT"
     gene            13710..14474
                     /locus_tag="VFJ72_05150"
     CDS             13710..14474
                     /locus_tag="VFJ72_05150"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7478887.1"
                     /translation="MAPVIGITATLKEDVDQIATRPLGRFVRADLDYVEGVAHAGGVP
                     VVLPPVGEQRAAEAVIGGLDGLLLSGGSDLDPSYYGEKPIPELNVTLPERDAFEMALL
                     GEALRRGIPIFGICRGLQVLNVALGGTLYQDLPAQLGREILAHRQETPKWQPTHEVEI
                     HEGSWVGGITESTCVKVNSYHHQSIKDLADGLRVSGRSSDGVIEAIESHDFSERWLVG
                     VQWHAEAMRDAGAEHRNLFHAHVQAAEHHALRRAAA"
     gene            14464..15135
                     /gene="tmk"
                     /locus_tag="VFJ72_05155"
     CDS             14464..15135
                     /gene="tmk"
                     /locus_tag="VFJ72_05155"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7478888.1"
                     /translation="MRPRETRRSAASRGVFVTVEGLDFSGKSTLVRHLKRVLADADPP
                     MRFTREPGGTPAAERIREVLLDPDLDMDPWTEAYLYAAARADHARLQIQPSLERGENV
                     LCERYLDSSIAYQGSGRGLGVAAVRELNARAVGGVLPDRTFYLRLDAAERERRAKSLG
                     APLDRIELVGADFMRRVEEGFEELALSEPDRIVVLDASLLPEKLAELVVGEIQGLQYT
                     RGNGG"
     gene            15239..16336
                     /locus_tag="VFJ72_05160"
     CDS             15239..16336
                     /locus_tag="VFJ72_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478889.1"
                     /translation="MTETRTFDGILGNAPALTLLERAVERGEVAHAYLFGGPAGVGKR
                     TVARRFGAALVSDGDAAVEDRARRGMHPDLVEVRPEGAFTTIGQVREVVRVAASRPFE
                     GARRVFILEADTFNVQAANALLKTLEEPEGETVFVLLAASREGVMPTISSRAQTVRFN
                     PVAVKEVAEFLRERGVEEPELAAALGRGSVGLSIRYAEEPEMKELRGAVFEAGFSFDE
                     DFETRHRAVSRIVARAEAVGAAREAAYLESFEEPDRRAKEGAKRAGRAARDGAVREAL
                     ELLALLYRDAAAVDVGAEDLVANVDRVTEIRARVDQYPGADWAGAALALGEARAGLAY
                     NVSPEAILEVTLSRIRRRILDTYPAESYPAS"
     gene            16360..17190
                     /gene="ricT"
                     /locus_tag="VFJ72_05165"
     CDS             16360..17190
                     /gene="ricT"
                     /locus_tag="VFJ72_05165"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HET7478890.1"
                     /translation="MPRLCDARDLDLRVGYRVVVDTEYGEFVGRVALTPRRREPYTEP
                     YDILRVVTREDEREEDAHRELGREIRKEAVKLARDHQIGAVEFIGCDVSLDGSYIEVK
                     YQADGERDLARVRDGLEGLYDARVEMRRFSFIERSGDTSGCDTCGLPLCCATWSGARS
                     MGPVNVRLAKQQGVTPNEKIIGCCGEVKCCMRYEHDAYKEFKERAPFKNTVVKLDERE
                     GKVTDYSMVRDSVFVQFGPKRSDQELVPLGRLARDNPDILPADPEDWEVVPASESPDE
                     "
     gene            17198..18154
                     /locus_tag="VFJ72_05170"
     CDS             17198..18154
                     /locus_tag="VFJ72_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET7478891.1"
                     /translation="MSGNLCDVPGVLVGHATDTAGLTGCTAVLFDRPAVVGVDVRGSS
                     PGTRETDRLGPIGAVRPTHALLLTGGSAFGLAAAEGVVRFLEERGVGLDIGVAKIPLV
                     SAAVLFDLVVGSPTARPDAPMGYLAASSAVNGAFAEGSVGAGTGATVGKVLGMDRAMK
                     GGVGSASVRLDDGLVVAALAAVNAFGDVRDEGGSIVAGPRLDDGTLGDTLKLLPEAAA
                     RMRWGENTTLGIVATNAALTKPRATKVAQMAHDGLARAVYPVHTTVDGDVVFAASVGE
                     VEAVPDVVGAWGAHVMQEAILRAVREAEGVAGIPSVKDHQRG"
     gene            complement(18218..19279)
                     /locus_tag="VFJ72_05175"
     CDS             complement(18218..19279)
                     /locus_tag="VFJ72_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7478892.1"
                     /translation="MKAARLHKYDASIPSESLKLEEIEEPKADGPFDVIVKIGAAGLC
                     RTDIHIIEGQWAPIQDADGNLLPYVLGHENCGWVEEVGPAVTNVAVGDTVILHPLITC
                     GLCRYCRAGRDMQCENGAFPGISQDGGFAQLLKTSARACVKLDPILQPKDIAALADAG
                     LTAHHAVRKSVPELYAGTNAVVIGAGGLGHIGIQCLKAMTPANVIVVDPNEEALALAK
                     EWGADDTVKVEDGYVDTVIEMTNGGAEVVFDYVGERGAEDDAWKMTRPAGSHYVIGYG
                     GTVEVPTIDIISTERSVVGNLVGTYNDLAELMNLTARGLVKLHTATYPLDAVNDAIQD
                     LNNGNLRGRGILIPEGAES"
     gene            complement(19276..19971)
                     /locus_tag="VFJ72_05180"
     CDS             complement(19276..19971)
                     /locus_tag="VFJ72_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET7478893.1"
                     /translation="MPTREAVLDALSGVRDPELDEPITDLDFVSGLEIEDGAAFVRLR
                     LPTYFCAPNFAYLMVADAKAAVLSVAGIEQARVVLDDHYASDEINGGVNEHRGFDGAF
                     PDKTEGGDLEELRTTFRRKSFVSRQEQLCRAMLSEGHTPLGLAGMSLQDVPPSGEFET
                     YLQRRAELGLDIGPSAPLIVDPDGNRVPSEAVVQHLRFAKTVRVSIEGNASLCRGLLA
                     TRYGIQEKEEATA"
     gene            complement(19971..>20882)
                     /locus_tag="VFJ72_05185"
     CDS             complement(19971..>20882)
                     /locus_tag="VFJ72_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7478894.1"
                     /translation="NLSPDEYTWTFEKYRKYSEEDMMHDLFEVGYVDKAILQSTYLTD
                     FYDNGFNTIERNHTLVDKYPDKFLSNFGWDPRNEEKGLEMLERDFEKYGSKGVKLYTA
                     EWKGESHGWKLDDPWSLKHLEKCQELGIKNVHIHKGPTIWPLNRDAFDVADVDRTATL
                     FPDLNFVVEHVGLPRLEDFCWIGTQEPNVYGGLAVAMPFIHAKPRYFAQIIGELLYWI
                     GEDRLMFASDYALWQPKWLVEKFVDFQYPEDPEFDDYAEITPDIKRKILGLNAAKLYD
                     IEVPEEMQLATAGSATGQIAAEPANPA"
BASE COUNT         3671 a         6371 c         7228 g         3612 t
ORIGIN      
        1 gctcgagctg ctcgccgacg ttgcgctctc gcacgcgcgt gcgggtgctg acgtggtcgc
       61 gccgagcgac atgatggacg ggcgcatcgg tgcgatccgc gcgcagctcg atgccgagca
      121 ctgcgagcac gtgtcgatca tgagctacag cgccaagtac gcgagcgcct actacgggcc
      181 cttccgcgag gcggccggct cgacgccaca gttcggcgac cgccgcgcct accagatgga
      241 ccccgccaat gcccgcgagg ccctcgtcga gacggtcctg gatatcgagg agggcgctga
      301 catagtgatg gtcaagcccg cgctcccgta cctggacgtc ctgcgccgcg tgcgcgagat
      361 gaccaacctg cccgtcgccg cctacaacgt gagcggcgag tacgcgatga tgaaggccgc
      421 cgtccagaac ggctggctgg acgaggagcg ggccgtcatg gaggcgctga ccggcataaa
      481 gcgcgccgga gccgacataa tcatcagcta ccacgcgccg gacgtggcga gttggttggg
      541 tgaatagtcg gcggggggag gtttgtgact cgtaaggtaa tggtctggcg ggaggatggg
      601 agtcgtcccg ttctgacgga ggtgcttgcc gacgacgaat ttcagcttca agaaatcgtg
      661 aaggacacgc cggatctctt gccggtggac gagtttggca tgaccggtcc gctgatgatt
      721 gtcgggcgtg agacaacgct tccgtcgggg tcggttgacc tcattggagt ttctcgggat
      781 ggagaacttc tcctcatcga gttcaaaacc gggcctcaaa attccgactt tcgtcatgta
      841 ctggcccaac ttttggatta tggttctgat ctctggcgaa tgtcgtacga ggagtttgag
      901 agtacggtag ccagacgctt cttttccggc gaccattgcc gggatgcgcg gcttcgagga
      961 aaggcttcaa ttatagatgc ggccctcgaa atttggcccg atttgtcgga tgaggaaaca
     1021 gcatctcttc gtgagaggct ctcacagcaa cttgaaagcg gtgagttcca ctacgtcgta
     1081 gtcgctcaga gattcagaga tacgatggaa cgaaccgtcg aatatttgaa ctctacgatg
     1141 cgaggcgcca gattttatgc cgttgagttg gtgcgattct ctgcagggaa tctttcggca
     1201 ttcgaatccc ggactgtatt gaaacctgaa agtcatggtt ccggttcgac tcaacgaacc
     1261 cgcacgagcg agacttcacg gacgcgaacg aacgaaattc agttcctaga gagggtcgaa
     1321 gacgacgcat accgcgaaac actccgggat cttttggacg tatgccacgg actaggcttg
     1381 caattttcgt gggggccggc cggaacctcg atccggatga cttctctgga tcgcccgagg
     1441 ccgttatcca tagcgtggct ctttcctccc ggtgtctccg gctggatggg cttgacagac
     1501 ctcacgctcg gacatagtga gaatcaagaa gatgtacctc aagcggtgca gaattacgtt
     1561 gaccgcgtat caaagttgcc aggtgtcgaa gttgccaaac ctaagtggct cagcggctat
     1621 cgcctcggcc ccgagacggc gatacaaaac catcatcaga tagtcgaaat actcgctgag
     1681 cttgtcagcc agtccggcga agaaacgtag cgagtgcgag cgaacacgaa cgtcgaacga
     1741 ccattgcagt aaacttttcg ctaccttttc cgaagggagt ccgtaccctt gcaatatagt
     1801 gactcagacg caagaatcac gatctcaatg gaccgctcgg cccgcctgat ggagcgggcg
     1861 gtacagagga tgcccggcgg cgtgaacagc ccggtccgcg cgttccgctc ggtcggcggc
     1921 gacccgctgt tcatggaacg cggcgagggc tcgcgcatct tcgacgtgga cggcaactcc
     1981 tgcgtggact acgtaatgag ctacggtcca ctcgttatgg gccacgccca cccggaaacc
     2041 atagaagcca tcgagcgcac cgccaggaaa ggtacgacat tcggtgcccc gacggagctg
     2101 gaagtcgagc tggcggaact ggtctgcgag tctgtgccgt ccgtagagat ggtcagaatg
     2161 accaactccg ggacagaggc gacgatgagc gccataaggc tcgcgcgcgg gtacacgggg
     2221 cgcgagaaga tcctgaagtt cgacggcaac taccacggcc acggcgacgc cctgctagtc
     2281 gctgctggat cgggcgtcgc caccctcggc ctcccggaca gccccggcgt tacgaagggg
     2341 gccgcgaagg acaccgccgt cctgccgtac aacgacctgg aggccgtcag agaactcttc
     2401 gagcgtgagg gcgaggagtt cgccgccgcg atcctggagc cggtcgccgg caacatggga
     2461 tgcgtgccgc ccgccgaagg ctacctcgaa gggctgcgcg agcttaccgc cgaatacggc
     2521 gtcgtcctca tcttcgacga ggtgatgaca gggttccggc tcgcgcgtgg cggcgcgcag
     2581 gagaggtttg gggtgacgcc ggacctgacg acgctcggca agatcatcgg cggcggcctc
     2641 cctgtcgggg cgtacggcgg gaagcgggag atcatggacc tcatcgcgcc ctccgggccg
     2701 gtctaccagg cgggcacgct ctccggcaac ccgctggcga tggccgccgg cctcaccaca
     2761 cttcgcgcca ccgccgagcc ggacttctac gagcggttgg agcagagcgg ggagcgctgg
     2821 agacagggga tgaacgaggc tgcatcatca ggtggagtgc cgttcacgat aaaccaggtc
     2881 ggctcgatgg tgagcatctt cttcaccggg ggtccggtca cggatttcga gacggcggcg
     2941 cggtcggata tagaggcgtt caaggacttc ttctggcaca tgctctcgcg tgggatctac
     3001 ctggctccga gccagtacga ggcgggcttt atctccaccg cccacactga ggaggatctg
     3061 aatcggacct tcgaggccgc gggcgagtgg ttcgcgggac gcggctagat ggagcgcgcg
     3121 gctcgccgaa ccccggaggt ctcgctggaa gaggtcttcc gccgggcttc ccggggagta
     3181 ccggatgagg aagaacgggc cgccgtggtt gcggaggcgc tcggatattt gcagcgcggc
     3241 ttcgattctc attacgcctc tggtgagaga gctacggatg atgagattct ggtcggcgac
     3301 aatgcctacg cctgggcggt ggagactatc tcgcgcctgg acgagccgga gttcgtggcg
     3361 gtcgcgagcc gtatgatccg ggacggcgcg ggccgcgtct ctgcgggcgg tggcgtggac
     3421 ctggagctct ggacgccgca cctggctgac ctgctccgga tcatctccgg cgaagacccg
     3481 gagcgttcca gagagaggat cacggccgcc gccggggagg tggccggtga acggagcgcg
     3541 taagggacca tcacccgccg gaaaacttcc atccggaacg gaacgggcgc ggctcgtaca
     3601 ggagatgttc gaccgtatag cgggccgcta cgagctgctc aacaccgtaa tgaccgccgg
     3661 gctgaaccgt atatggaaca ggaaagtcct cgcggcgacc ggcctgcgcc ccggcgaccg
     3721 cgccctggac ctggcctgtg gcacgggcag cctgacccgc gaccttgccc gccgcgtcgg
     3781 acccgagggc tctgtcctcg gcgtggactt ctcgcgcgag atgctccggg ccgccgaacg
     3841 ccggccgctc ccgaacatcg agtaccagct cggagacgcc acgaggctcg acggcgtccc
     3901 gtcagaagca ttcgacgcgg cgacgatcgc ctacggggcg aggaacatcc cggacctcga
     3961 tgcgctgttc gcggagatgg cccggtgcgt gaggccgggc ggagcggtcg tctgcctgga
     4021 gatagcccgg ccggcgggga ggatagcctc cgcgttctac gggttgtggt tcgataaggt
     4081 agtgccgcgg ctgggggcga ggatctccgg tgatgcctgg gcttactcgt atctgccgga
     4141 atcggtgaaa gagttcgtgg cgccagacga gttagctgtc ataatgagac gtaatggatt
     4201 acaaagcgtt acatggagcc ggctcgcggg cggtatcatc accctccacc gtggattccg
     4261 gcgtgcatag cttcgagcag agtgccagcg acctgacccg cgcgctgccg gatgcagctt
     4321 tcgaggtgtg gcaggaagtt gaggaccggc tcgtcgtggt tggggagcgc gccccggcgg
     4381 cgttggcgca gccggcgctc gaatcgctga cctccggggg gaagaggctc cggccgctgc
     4441 tgctcatttt gagcgcgagg ctgggcgagc caaataggga gtcgctgcta cgggcttcga
     4501 cggcgatcga ggtgctgcac acggcgacgc tcatccacga cgacatagtg gaccgggcgg
     4561 agtcccggcg cgggcagccg acgaccgtgg cggcgtatgg aagagagatc gcgatagcca
     4621 ccggagacta cctcttcgcg gagtcgttct cggagctggc cgggatcggg gaccccaggc
     4681 tcgtccgggc gttctccgag gcggccgagg ggctggcggc gggggagctt gagcagtacc
     4741 gggcgagtgg cgctccggtg gagcttgagg cttacctgga ccacatcagg aagaagaccg
     4801 ccggactttt caaggcggcc tgcgtggcgg gaggtacgct tggtggcctc tccctgcgcc
     4861 agatagatgc tctggcgacc tacggcaacg cgctgggcat agcgttccag atgtcggacg
     4921 acgtgatgga cctcgtgggc aagccgggcc ttatgggcaa gggcatcggt acggacctcg
     4981 cggaagggac ggttacgctg ccggtcatct tcgcgctgca ggagggggat gcggagaaga
     5041 tccgggaggt gctcgccgat ccgcacccgt cgccggacct gctggaggcc gggatcgaag
     5101 ccgtgctcgc caccgacgcc attgagaaga ccgaggcgtg ggcgcgcggc gagatagacg
     5161 ccgccgtgga cgacctgagt atcctgccgg acacggcgga gcgcgcgatc ctggaggccg
     5221 tggcggcgga ggtcgtcgga agagatgcct gagaaatcag tgatattcgc cgccgacacc
     5281 gtgttcccgg tctccgctcc cgctgtcagg aacggggcgg ccctggtggc tcccgacggc
     5341 cgcatcggcg cagttgggac gctcgaagac ctgcgacgcg cggagccgga tgcggaggtc
     5401 cgggagtttc cggggtgtgt gatcttgccg ggcgccgtga acgcgcacgc gcacctgggt
     5461 ttccgtcgca aggacgcgcc ggagggtggc acgttcgcgg gctggctcgg gcggctaata
     5521 gaaaagctgc cggagaagga ggcgtggacg gcggaggccg cgcgggactc ggcgcgcgag
     5581 gcgatagagg ccgggacgac gttcatggca gagtcatcac cctacggtga atgcctgccg
     5641 cagctcgcgg agagcgggat ggcgggcgcc gtctacgcgg agttcttccc cggcgacttc
     5701 gatggggcgg gttccgagga gatcgtggat tccatcattg gaaaagtgaa ggatctcgga
     5761 gaaggtctgc cggagcgcgt tgcggcgcgg gtcagcgtcc attcgccgta cacggttgac
     5821 ccggagtcct cgcggctcgc ggcgcaccgg acgcgggaga tgggctggcg gctggcgata
     5881 cacctctccg agagcccgga ggaggtggag ttcgtccggg acggcacggg cgggctcgcg
     5941 gatatcttcg ggaagaacga gtggggcggc aggggcgtct cgccggtccg gtacgccgag
     6001 gagatagacc ttctcgcgcc cctgacgata gccgcgcacc tcgccacggg cgtctcggac
     6061 gacgacatcg aggtccttag ccgcagcggg gtcgccgcgg cccactgccc gcgctccaac
     6121 gatttcctcg gatgcgaggt ctcgcgggta tcggagatga tgcgccacgg cgtccgggtc
     6181 gggatgggca ccgacgggct gtggagcagc ccgagcatga acctcttcga ggagacgctc
     6241 ttcgccgtga ggctgcacgg cttcgacggg gccacggggc tccggctcgc ctccctcgaa
     6301 ggggcgcggg ccctcggcat cgagcacgag accgggagca tcgaggccgg gaagtgggcg
     6361 gacctcgcgg tggtggaggc cgggcctggc gggaacgacc cggagcggga cgttctcgag
     6421 tcggcggccg gtgggggcgt cgcggccact gttgctggcg gcaacctgat atacaatcgg
     6481 gtctgagtcc aagacccgga ggaacttatc ccgccatgat gatgaaccgc gacaggctag
     6541 gcttctggac gaaagtcttt gccatcgggc tcgcggccgt gttcttgctg tcgtttatct
     6601 tcatgggcgt ggggacgaac attaactaca acctgttcga cctgttcggc ggtaacaaga
     6661 acgccaagca gggcggacag accgtctcgg cgcaggagca gatccggcag gccgaaaaac
     6721 aggtcgagaa gaatccgaag gacccgcagg cggtgcagac gctcgcggcc ctctactatc
     6781 agaacaaccg gccggacgac gccacgaagg tgctcgaaaa gggccgtaag gacatgccga
     6841 agaacgcgga tatagcgaat acgctcggtt cggtctacgc gcagcaggcc caggcggcgt
     6901 cggataaaga gaaagagaag ctcttcgcga aggccggcga ctccttcgcg gacgcgacga
     6961 agctggagcc caagagcgcg gactcctact acttcgccgg gcaggcctac gatcaggccg
     7021 gcgactccgc gaaagctatt gagtactaca acgggtatct ggaccgggcg ccgaagggag
     7081 atcaggcgaa agccgtgaag gaccgtatct cgcagatcct gaagggcgaa aataccaccg
     7141 gagcgtctgg cggaggctag agaaatgatc cgccgcgcca cttcaggtcg tataatgtcc
     7201 gaagtggttc tgagtaagta ccgttaggag gagagagatg ttcggatcac taggaccgac
     7261 ggagcttatt atcgccctcg tcatcatcct tttgctcttc ggagcgaaga aggttccgga
     7321 gcttgcccgc ggactcggta gcggcgtgaa ggagtttaga gccggcaccc gcgagggcga
     7381 ggtgaaggac aaggagaaag agaaggacga ggaactggcc gccgccgaga gcaagaccga
     7441 taagaccggc gaggcgcacg acccgcacct ggaagaggac gccgagaggc cccgagatgt
     7501 cactgccgag gccgaccggg ccgagcagaa gcagcgctag aaaccgcgaa gattctctaa
     7561 cctagatggc aggacgcctc agactaccca ctcgctcgcg cgaagcgcgc ggcgaggcgc
     7621 gcatgaccct gatcgagcac ctggacgagt tccgctccag gctcatcaag gtcgccatcg
     7681 cgttcgtggc ggtctccatc gtcgcgtggt tcttccggga ccgcatcttc gacgcgcttc
     7741 tcgccccggc gggagacgcc ctgcacggca agctcaacgt aaccggcgta acggagcagc
     7801 tcttcaccga tatgaagctg gcgctctacg tcggtttcct gctcactatc cccatacttc
     7861 tctaccaggc gtgggcgttc gtcgcgccgg ccgtcggaga cctgggacga gcattcacct
     7921 acacgatcat cacgctcgct tcggtactgt tcctggccgg ggtcgccttc gggtatttct
     7981 tggttctccc gataggcacc cagttcctgc tcagctggga ccccgaccgc tacgacacca
     8041 tcatcacctc tcagagttac ctgccgttcg ttacgcggtt cctgctggcc ttcgggatcg
     8101 tcttcgagct gccggctgcg acttacgtgg ccgcgaagct ggaactggtc gatgctccgt
     8161 ttctgaaaaa gtaccggcgg cacgccatcg tgctaaacac catcctcgcc gcagcgctca
     8221 cgccggggca ggacccgtac agcatgatcc tgatggccgt gcccatgatc gttatgtacg
     8281 aggcgagcat cttgatcgcc cgctacgtca acccggtctc cgaggtgagg gtccgggaac
     8341 tcggcgaaga ggacgacgcc gatcacgatg agatcgagga gcctgaggag cgagacctct
     8401 agctcgccgg aatcttctcg tcaggccagc cctccggcca gcacccctag cgtgtatagc
     8461 aagccgaaga cggcgtggaa cccggcggtt gccttgacga ccgggtccag ccgggccggg
     8521 accgtgctgg acgtgatgtt gcgccagagt ttggcgacca tcggcaggct cacgagcgcc
     8581 atggcgcact cggccgggac gatgcccagg aggatcaggg cgatcagtgc cacgtaggct
     8641 tcgaggagca ggagcctgta gatgaggatg gccctgcgtc gccccaagac tatcgggagc
     8701 gtccggcgtc caccgcggcg gtccgagttc atgtcccgga tgttgttcgc cagcaggatg
     8761 gcggccacgg gaccgccgaa cgggagggcc gcaaacggta cgtagagcgg gaactcgccc
     8821 gcctgtacgc cgtaggtgat caccacgatg aggataccca tgaagataaa gacggccgcc
     8881 tcgctcgccg cggtgtaggc gatgggccgg ggaccggcgg agtagaggta gccgccgagc
     8941 gccgagagac atcccaggac cagtatcggc cagccgccca cggcgatgag gtagatgccg
     9001 cagaacagcg ccagcgtgta gcagaacagc gctccgatga ggacggagcg ggcgtgcatc
     9061 cggccctgca ctatcgagcc ggcgatccct actgcgcccg ccgtgtcgag accgcgcttt
     9121 tcgtcgtaga actcgttgaa catgtttgtg gccgcctgga tcaggaggct acccgagagc
     9181 attgtcagga aagctccgcc cctgaacgcc ccgtccgcga gcgcgagcgc ggacccgaac
     9241 agcaccggca gcgccgccgc cgtcaggctg aagggccgcg ccagccagaa ccactcggcc
     9301 aggacggaac gtccgcgacc ggccgccatc aaacggactc cgggacgctg gcgcgaattg
     9361 cttgcggctt cgatactctc acgtgggtaa tttaaccaac ctgaagatgt gctgctgtga
     9421 aggaatacgg ctaatgtttg cggaatagag actttcgggg cgttttgtga tagaaacccg
     9481 ctggcatgga agcaggcggc accacgggat tcgcgggctc ctttctccgg ctggcgcggc
     9541 caaaacagtg gaccaagaac gggttcgtta tggccgggct ggtcttttcg gggaaggcac
     9601 tctcggcgtc cccggtatta gacgcggtac tggccttcgc cgctttctgt ctcctctcca
     9661 gcgctgtcta cgcggctaac gatgttcttg atatcgagga ggaccgcaag cacccgctca
     9721 agaggctccg gcccgtggcg agcggcgaga tatccgctcg ctccgcgctg acgtttgcgt
     9781 tcctgctggc ggcgggagga cttgctgtag ctttctttgg agtaaatccg ggggtgggga
     9841 tcgcggggct cgcgtatatc ggtcttcagg cggtctacac cacgctgctc aagcacatgg
     9901 cgatcctcga cgtaatgagc atctcggcgg ggttcgtgat ccgggctctc gctggcgtgg
     9961 tggctgtggg cagccccgtc tcgccctggc tgatcgtgtg taccggcctc ctgacgctct
    10021 tcctgggctt ctcgaagcgc cggcacgagc tggcgacgct gggggagcgt gcggctatcc
    10081 accgccggaa cctccgggac tactcggtgc cgatgctgga cgagatgatg aacatcatgc
    10141 tatcggcgac gatcataggg tacgcgctct acacgttcac ggtttacagg agcgagcagg
    10201 agatcttcat gatgtcgagc atcccgttcg tgatatacgg tgtcttccgg tatatgctgc
    10261 tcgttcaccg cgatggcggc ggcaacccgg acatgttgct gctcagggac cggccgctcc
    10321 aggtctcgct gctgctctgg ctcgcggtcg tcatgacggt catctacata ctctagggcc
    10381 gtgcagcgga tcggtagaaa cctgaccctg gcgctcggtc tcggcgtggc cgtgtacctg
    10441 gtcctcgccg tcttctcggg cttcgatgac ctccgggacg cgctggagaa cttccgctgg
    10501 tcgctcgctc cggccatcct gggcctcgtc gccgtctcct acacgggacg tttcctccgc
    10561 tggaggtact acctgcggct gctcaaggtg tccgtgccgg cgccggtgaa cgcggccatc
    10621 ttcgcggcgg gcctctccat ggcgatctca cccggcaagt tcggcgaggt gctcaagagc
    10681 gttttcgtcc ggcaggtctc cggcgccccg atagcccgca cggctcccgc cgtcgtagcc
    10741 gagcgcgcca ccgacggcac ggggatggtc gcctgggggc tactgggcgc cctggccttc
    10801 agcttcggac cttgggtctt gatccttttc ctggccgtta ccgtcgcggg catcgcggtc
    10861 ctccgttcgc gccggctctc cctgctggcc gagcggatac tctcccgctt gcccctgctc
    10921 aatcggcttg cgccgcatct tgaggacttc cacggcgcgt ccaacgtgct gctcggcacg
    10981 cggcccctga tcgttgggac ggccatctcc ttcctctcgt ggggactgga gatcctggcg
    11041 gtatatctct gctccgtcgg aataggcgcc ggcctgccgt tcttgatggt cgtcttcgtc
    11101 ttcgcggtcg ggtctctgat cggcgtcggc agcatgctgc ccggcggcat cggggcggcg
    11161 gaggcgagca tggccggcat gttcaagttc tacgccgggc tcgccgccgg accagcagta
    11221 gcactgacgt tgctgatccg tctggcgacc ctctggttcg ccacgctcat cggcatagcg
    11281 ggcctgttga tagtgcgagg cgtaatcggt gatatcgtcc cggacgacgt tgccacgcaa
    11341 cccggcaccg agcggtagcg tctggtgatc gagcttacgc cagtccttgt ggcctgttct
    11401 acaagctact gcattgtgtt ataaccagga tcaatatgcg actaataatc tctgagaaag
    11461 cgaacgccgc gaagaagatt gcccagttcc tggcggaggg gtccgtcaag gaggggaagt
    11521 atcgtagcgt tcctcatcat acgttcacct ggaagggcga ggagtgcgtc tccgtcggcc
    11581 tgaagggcca cgtcctcaac cccgagtacc cggaagagta ctccaactgg cagaaggtcg
    11641 agccttcgaa gctgatcgac gccgagatcc tcaagtccgt ctccgagaag ggcgttgccg
    11701 aggcgatcaa gtctctctcc aagaaagccg accgggtcgt agtggcgacg gacttcgacc
    11761 gcgagggcga gttgatcggg gtggaggccc tctcgctcgt cttcgaggcg aacccgaagc
    11821 tgatggacca cgtcgagcgg tcccggttct ccgccctcac gccgggggag gttaaccgcg
    11881 ccttcgacaa cctcgtcgag gtctcgcggg agcttgcggc ggccggtgag gcgcggcagg
    11941 acattgacct gatctggggc gccaccctca cgcggtgggt ctcccgcgcc accaagcgct
    12001 acggtagcgc gttcctctcc gtcgggcgcg tccagtcgcc gacgctggcc ctgatcgccg
    12061 cgcgcgagcg cgagcgccgc gctttcgtcc ccgagccgta ctgggagctg gaggcgacgc
    12121 tgaagaacgg cgagccgttc accgtccgcc acgccaccga gcgcttcaag gaagaggcgg
    12181 cggtccgcgc ggcgtacgag aacctcgccg agaccgccac cgtcaccgag gtcaaacaga
    12241 agtccgcgtc ccggcagcct ccggcgccgt tcaacacgac cagcttcctc acggcggcgg
    12301 cgagcatcgg gatcagccct tcgagggccg cgcggctcgc cgaagacctc tacacggacg
    12361 gctacatctc ctacccgcgg acggacaaca ccgtctatcc gcggtcgctg gatcttcggg
    12421 aagtcctcgg atacctcaag cgtgtcgagg gagccggaca acacgcggag aggctgctcg
    12481 cacaggagaa gctctctccg acccgcggca agaaagagac cacggaccac cccccgatct
    12541 acccgacagg atacgcctcg aagaaggcgc tccgggacga ccagtggaag atctaccagc
    12601 tcgtcgtgcg gcgcttcctg gcgaccctga tgggaccggc caagacgctc cgcaccacgc
    12661 tgcgcttcga ctccggcggc gaaccgctaa cgaccggcgg cacggtggta accgaggaag
    12721 gctggctcgc tgcatacccg tacagccgcc gcgccgacga ggagatccca aacctcagcg
    12781 agggcgaagt ggtgaaggtt gtcagttcgg agattctggc taaagagacg cagccgcccg
    12841 gacgttacgg cgcgggacgc ctgatcaacg tgatggagga cctggggctg ggcaccaaag
    12901 cgacgcgccc gaacatcata caaaacctct acgaccgggg ctacgtccac gacgacccgc
    12961 tcatcccgac tgagaagggg ataaaggtgg cgcaggccct tacggacttc gcctccgaga
    13021 tagcctcgca cgagatgacg gcggagcttg agcgcagcat ggacgcgata tcggagggca
    13081 agatcagcaa ggagaacgtc gtggatgagt cgcgcgacgt gctccgcagg gtctacgagc
    13141 acctccagaa ctcagagacc gagttcgcgg acatcgtgtg ggagggcatc cgcacggacg
    13201 agacgctcgg taagtgcccg gagagcgggc acgacctgat catccgccgc aatcgcaaga
    13261 gccgcaagcg cttcgtcgga tgttccggct acccggagtg taaggtcacc tacccgctgc
    13321 cgcagcgcgg cgagatcata ccgctcggca cgcagtgcga cgcttgcggc tcgccggaga
    13381 tcaaggttct cggcggtaag cgcccctgga taacgtgcat caacatggac tgtccgaaga
    13441 agcaggagca gaaagaggcc gccgcgaagg cggacgaaga ccggcaggag gtcgagcagc
    13501 catcgacaga gagagcgcag gaactggaga aatctaccac ctagaagacc ggatggaact
    13561 ctccaacctg gaaacgccac agaaccgagc cgcaaccctt cgcgcccgcg ctttcgggta
    13621 cgtgctgatc tggacgctct tcgcgatcgt atgctattct gcgaccttat agttaagata
    13681 cgtgaagatt ctttaaagga tgagttagct tggcacccgt tatcggcata accgcaactc
    13741 tcaaagaaga cgtggaccag atcgcgaccc gtccgctggg ccgttttgtc cgggccgacc
    13801 tggactacgt ggagggagtg gctcacgccg gtggagtgcc ggtggtgctg ccgccggtcg
    13861 gggagcagcg ggcggctgaa gccgtgatcg gcggtctcga cgggctgcta ctgagcggtg
    13921 ggagcgacct ggacccgagc tactacggcg agaagcccat cccggaactc aacgtgactc
    13981 tcccggagcg ggacgcattc gagatggcgt tgctcgggga ggcgctgcgg cgcggaattc
    14041 ccatcttcgg tatctgccgg ggtttgcagg tcctgaacgt cgcgctcggc gggaccctgt
    14101 accaggacct gccggcgcag ttgggacggg agatcctggc gcaccggcag gagaccccga
    14161 aatggcagcc gacgcacgag gtcgagatcc acgaggggtc gtgggttggc gggatcacgg
    14221 agagcacctg cgtgaaggtt aactcctacc atcatcagag catcaaggac ctggcagacg
    14281 gactccgcgt ctccgggcgt tcttcggacg gcgtgataga ggcgatcgag tcgcacgact
    14341 tctcggagcg gtggctcgtc ggggtacagt ggcacgccga ggcgatgcgg gacgccggcg
    14401 cggagcatcg caaccttttc cacgcgcacg ttcaggcagc cgagcaccac gcgctgcgcc
    14461 gggctgcggc ctagagagac ccggaggtcc gccgcgagcc ggggcgtctt cgtaacggtc
    14521 gagggcctgg acttctccgg caaatccacc ctcgtaagac acctgaagag agtcctggcg
    14581 gatgccgatc ccccgatgcg cttcacccgc gaacccggcg gcacacctgc tgccgaacgc
    14641 atccgggagg tgctgctgga ccccgatctg gacatggacc cctggaccga ggcttacctg
    14701 tacgcggcgg cgcgcgcgga ccacgcccgg ctgcagattc agcccagcct ggagcgtggg
    14761 gagaacgttc tgtgcgagcg ttacctggat tccagcatcg cgtatcaggg cagcgggcgc
    14821 gggctcggtg tggcggcggt tcgggagttg aatgccaggg cggtcggcgg tgttctgccg
    14881 gacaggactt tctatctgcg tttggatgcg gctgagaggg agcgccgggc caaaagcctc
    14941 ggagcgccgc tggacaggat agagctggtc ggggcggatt tcatgcggcg ggtcgaggag
    15001 ggtttcgagg agctagcgct ctccgagccg gaccggatag tcgtcctcga tgcttcgctt
    15061 ttaccggaga agttggcgga gttggtggta ggggagatcc agggtctgca atatactcgg
    15121 ggcaatggcg gctagttatt cggggacggt gtggaccgga gaccggacag cgagataatg
    15181 gggctttaca tggcattggg cgtcggagcc gcgctctcga accgtggaga cggcgaggat
    15241 gactgaaacg aggaccttcg acggaatatt gggcaacgcg ccggcgctaa cgctgctgga
    15301 acgggcggtg gagcgcggcg aggtcgcgca cgcttacctt ttcggaggac cggcgggtgt
    15361 gggcaagcgc acggtggcgc ggcgtttcgg ggcggcgctc gtctcggatg gagacgcggc
    15421 ggtggaggac cgggcgcggc gggggatgca tccggacctc gtggaggtcc ggccggaggg
    15481 cgcgtttacg accatcggac aggtccggga ggtggtgcgc gtggccgcga gccggccgtt
    15541 cgagggggcg cgtcgggtct tcatcctgga agcggacacg ttcaacgtgc aggcggcgaa
    15601 cgccctgttg aagacgttgg aggaaccgga aggggagacg gtcttcgtgt tgctcgcggc
    15661 atcccgcgag ggtgtgatgc ccacgatctc ctcccgcgct cagacggtgc gcttcaaccc
    15721 ggtggcggtg aaggaggtcg cggagttctt gcgggagcgg ggtgtcgagg agccggaact
    15781 ggcggcggcg ctggggcggg ggagtgtggg gctctcgatc cgatacgccg aggaaccaga
    15841 gatgaaagag ttgcgcggcg cggtcttcga ggcggggttc tcgttcgacg aggacttcga
    15901 gacgcgccac cgggccgtct cacgcatcgt ggcgcgggcc gaggcggtcg gggcggcgcg
    15961 agaggcggct tacctggaga gtttcgagga gccggatcgc cgggccaagg agggcgcgaa
    16021 gcgggccgga cgtgccgcgc gggacggggc ggtgagggag gcgctggagt tgctcgcgct
    16081 gctgtaccgg gacgcggcgg ccgtggatgt gggtgcggag gacctcgtgg cgaacgtgga
    16141 ccgggtgacg gagatccggg cgagggtgga tcagtacccg ggcgcggact gggcgggggc
    16201 ggcgcttgct cttggggaag cgcgcgcggg ccttgcgtat aatgtctctc cagaggcgat
    16261 cctggaggtt accctatcgc ggatacggcg cagaatactg gacacctacc cggcggagag
    16321 ctaccccgcc tcgtagacgt ccgcgtctcc gggtggagcg tgccgcggct ctgtgacgct
    16381 agagacctcg acctgcgcgt cggttaccgg gtcgtcgtgg atacggagta cggagagttc
    16441 gtcggtcgcg tagccctgac tccccgacgc cgcgagccgt acacggagcc ctacgacata
    16501 ctgcgggtcg tcacgcggga ggacgagcgg gaggaggacg ctcaccggga gcttggacgt
    16561 gagatccgca aggaggccgt aaagctcgcc cgcgatcacc agatcggcgc ggttgagttt
    16621 atcgggtgcg acgtctctct cgacggctcc tacatcgagg tgaagtacca ggcggatggc
    16681 gagcgcgacc tggcgcgggt gagggacggg ctcgaaggtt tgtacgacgc ccgcgtggag
    16741 atgcggcggt tctcgttcat cgagcgctcg ggcgatacga gcggctgcga cacgtgcggg
    16801 ctgccgctgt gctgcgcgac gtggagcggg gctcggagca tgggtccggt gaacgtccgg
    16861 ctcgccaagc aacagggcgt gacgccgaac gagaagataa taggctgctg cggcgaggtg
    16921 aagtgctgta tgcgatacga gcacgatgcc tacaaggaat tcaaggagcg ggcgccgttc
    16981 aagaacacgg tagtgaagct cgacgaacgc gaaggcaagg tgacggacta ctcgatggtc
    17041 cgggattccg tcttcgtcca gttcggtccg aagcgcagcg accaggaact ggtgccgctc
    17101 gggcgtctcg cccgcgacaa cccggacatc ctcccggcag acccggagga ctgggaggtc
    17161 gtcccggcct ccgagagccc ggacgagtag cctcagagtg tccggcaacc tctgcgacgt
    17221 gcccggcgtg ctcgtcggcc acgccaccga caccgcgggc ctgaccggat gcaccgccgt
    17281 cctcttcgac cggcccgccg tcgttggcgt ggacgtgcgg ggctcctccc ccggcacgcg
    17341 ggagacggac cggctcggtc ccatcggcgc ggtgcgcccg acccacgccc tgctccttac
    17401 cggcggcagc gcgttcggac tcgcagcagc cgaaggagtg gtgcgcttcc tcgaagagcg
    17461 gggcgtcggt ctggacatcg gggtggcgaa aataccgctc gtctcggccg ccgtgctctt
    17521 cgacctggtc gtcggctctc cgaccgcccg gcccgacgcc ccgatgggat acttggccgc
    17581 gtcctctgcg gtaaacggag cgttcgccga gggtagcgtt ggggccggga ccggcgcgac
    17641 ggtcgggaag gtcctcggca tggaccgggc gatgaagggc ggcgtcggga gcgcctccgt
    17701 gcggctcgac gacgggctcg tcgtggcggc gctcgccgcc gtcaacgcct tcggcgatgt
    17761 acgggacgag ggtggaagta tcgtggccgg tccccggctg gacgacggga ccctcggcga
    17821 caccctgaaa cttcttcccg aggccgccgc ccggatgcgg tggggcgaga acaccaccct
    17881 cggtatcgtg gctactaacg ccgctctcac aaagccgcgg gccacgaagg tcgcgcagat
    17941 ggcccacgac ggcctcgccc gcgccgtcta tccggtgcat acgacggtgg acggcgacgt
    18001 ggtcttcgcc gcgtccgtcg gagaagtcga ggccgtcccc gacgtggtgg gcgcgtgggg
    18061 cgctcacgtc atgcaggaag ctatcttgcg ggccgtccgg gaggccgaag gcgtcgccgg
    18121 aataccctcg gtcaaggatc accagcgggg ataagaaaga ctcgcggccc ggacatcacg
    18181 cctgggccgc gaggaatttc cctatctggc gcggctacta gctctccgcg ccctcgggga
    18241 tgaggatgcc gcgtccgcgg aggtttccgt tgttgaggtc ctggatggcg tcgttgacgg
    18301 cgtcgagcgg gtaggtcgcg gtgtgaagct tgaccaggcc gcgggccgtc aagttcatca
    18361 gctcggcgag gtcgttgtag gtgccgacca ggttgccgac gacgctgcgc tcagtggaga
    18421 tgatgtcgat ggtcgggacc tcgacggtgc cgccgtagcc gatgacgtag tgcgaaccgg
    18481 cgggacgggt catcttccag gcgtcgtcct cggcgccgcg ctcgcccacg tagtcgaaga
    18541 cgacctccgc gccgccgttg gtcatctcga tcacggtgtc cacgtagccg tcctcgacct
    18601 tgacggtatc gtccgcgccc cattccttcg cgagcgcgag ggcttcctcg ttggggtcaa
    18661 cgacgatcac gttcgcgggc gtcatcgcct tgaggcactg gatgccgatg tgcccgagtc
    18721 cgccggcccc gatgactacg gcgttggtgc ccgcgtaaag ctccggaacg gacttgcgga
    18781 cggcgtggtg tgcggtcagg ccagcgtcgg cgagggccgc gatgtccttg ggctgaagga
    18841 tcgggtcgag cttcacgcac gcccgggcgc tggtcttcag gagttgggca aagccgccgt
    18901 cctgggagat gccggggaaa gcgccgttct cgcactgcat atcgcggccg gcgcggcagt
    18961 accggcacag tccgcaggtg atcagggggt gcaggatcac cgtgtccccg accgccacgt
    19021 tggtgaccgc cgggccgacc tcctcgaccc agccgcagtt ctcgtgtccc agcacatagg
    19081 gcaggaggtt accgtccgcg tcctgtatag gagcccactg gccctcgatg atgtgtatgt
    19141 cggtacggca caggcccgcc gccccgatct tgacgattac gtcaaagggg ccgtccgcct
    19201 tcggctcctc gatctcctct agcttcaagc tctccgacgg gatgctcgcg tcgtacttgt
    19261 gtagcctcgc tgccttcatg ctgttgcctc ctccttttcc tgtattccat atcgggttgc
    19321 aagaagtccc cggcacagac tggcgttccc ctcgatgctc actctcaccg tcttggcgaa
    19381 ccgcaggtgc tggactaccg cctcgctcgg gactctgttg ccgtccgggt ccacgatcag
    19441 cggcgcgctc ggcccgatat ccagaccaag ctccgcgcgg cgctgtaggt aggtctcgaa
    19501 ctctccagat ggcggcacgt cttgcaggga catgccggcc agacccagcg gggtgtgtcc
    19561 ctccgagagc atggcccggc acaactgctc ctggcggctc acgaacgact tgcggcgaaa
    19621 cgtggtgcgc agttcctcca ggtctccacc ctccgtcttg tccgggaacg cgccgtcgaa
    19681 gccgcgatgc tcgttgacgc cgccgttgat ctcgtccgag gcgtaatgat cgtcgaggac
    19741 gaccctggcc tgctcgatgc ccgctactga cagcacggcg gccttcgcgt ccgcgaccat
    19801 caggtaggcg aagttcgggg cgcagaagta agtcggcagg cgcaggcgca cgaaggcggc
    19861 gccgtcttcg atctccaggc cggagacgaa gtccaggtcg gtgatcggtt cgtcaagctc
    19921 ggggtcccgg acgccggaga gggcgtccag gaccgcttcg cgggtcggca tcaggccgga
    19981 ttggccggct cggcggcgat ctgaccggtg gcgctgccgg cggtggcgag ctgcatctcc
    20041 tcggggacct ctatgtcgta gagcttcgcg gcgttgaggc cgaggatctt gcgctttatg
    20101 tccggtgtga tctcggcgta gtcgtcgaac tcggggtctt cgggatactg gaaatccacg
    20161 aacttctcga ccagccactt cggctgccag agcgcgtagt cgctggcgaa catgagccgg
    20221 tcctcaccga tccagtacag tagctcgccg atgatctggg cgaagtagcg cggcttggcg
    20281 tggatgaacg gcatcgccac cgcgagcccg ccgtagacgt tgggctcctg cgtcccgatc
    20341 cagcagaagt cctcaagcct cggaagtccc acgtgctcta cgacgaagtt gaggtccggg
    20401 aagagagtcg ctgtccggtc gacgtcggcc acgtcgaagg cgtcgcggtt gagcggccag
    20461 atcgtcggtc ccttgtggat gtggacgttt tttatcccca gctcctggca tttctccagg
    20521 tgcttgagcg accagggatc gtccagcttc cagccgtggg actctccctt ccactcggcc
    20581 gtgtagagct tgacgccctt cgagccgtac ttctcgaagt cgcgctccag catctccaaa
    20641 cccttctcct cgttgcgagg gtcccagccg aagttggaga ggaacttgtc cgggtactta
    20701 tccacgagcg tgtggttgcg ctcgatggtg ttgaagccgt tatcgtagaa atccgtgaga
    20761 taggtggact ggaggatcgc cttgtccacg tatcccacct cgaagaggtc gtgcatcatg
    20821 tcctcttcgg agtacttgcg gtacttctcg aaggtccagg tgtactcatc ggggctcagg
    20881 tt
//
LOCUS       DASSMB010000078         5746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1284561, whole genome shotgun sequence.
ACCESSION   DASSMB010000078 DASSMB010000000
VERSION     DASSMB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1284561"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1878
                     /locus_tag="VFJ72_05190"
     CDS             <1..1878
                     /locus_tag="VFJ72_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7478895.1"
                     /translation="KIEAGQMNIEIIDFDLRLTVEDTVTLLADRAHIKGLELASFVEP
                     DISSPLRGDPGRIRQVLTNLLGNAIKFTEQGEVVLRVETAGRRRGNQQSMRTVRFSVS
                     DTGIGMTEEQQEKIFASFSQADASTTRRYGGTGLGLAISRQLVEIMGGRIWVESEPGA
                     GSTFSFELPLMETDGGPVRDTSGVELSGLKALVVDDNATNRQILDRQLSSWGMASEGV
                     EGGAEALRELREAAGRGEPYDAAILDMQMPGMDGMRLAREIKQDPAISRTRLILLTSM
                     GRRGDGREALEAGIEAYLTKPVRQSELYDALATLMAADSTGDEDTDSRLVTRHTLREM
                     RSERGSRVLLAEDNDINQKVAVRMLERLGYRVDVVENGREALEALERDPSYGAVLMDV
                     QMPEMDGYEATAEIRRLEALEAYRLHVRGSCAGARSIPVIAMTANAMQGDREKALAAG
                     MDDYLSKPVKAEDLKAVLGRWVSPEAPGPAEADEPVKIESGTEPPPETPTESPNEPPL
                     DDAVLSALRELQEEGEPDIIAELAGLFVEDTVERLGTLREAIEAGDAAGVERAAHALK
                     GSSGNMGARGLSERSARLEEAGTAGALSEAKDLLESIESEFARVRLALEAEVQQAANK
                     R"
     gene            1944..>5746
                     /locus_tag="VFJ72_05195"
     CDS             1944..>5746
                     /locus_tag="VFJ72_05195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7478896.1"
                     /translation="MEMTGTYDLRLVALSILVAVIASYAALDLAGRVTASKGGARRVW
                     LASGAVAMGAGIWSMHFIGMLAFSVPMPMTYSAPTVALSLLVAIAASGLALFIVSRRT
                     MGRPQLVSGGLFLGAGILGMHYIGMAGMRMQAAVSYDPLLVGLSAVIAFGASLAALWL
                     AYQLRRESGPGGRWLRLKAASALVMGGAISGMHYTGMAAASFAPTRDVLKAPPGAAET
                     GTSILAAAVGAATLAILGVAIFTSVLNRRFLAQAAALEASEQRYESLFEENPDAVFSF
                     DREGRFTSVNPAVERITGYPADEILGQTSGRLLAPEDLERAAEHFMKTLRGEPRSYET
                     AVISRDGSRVPVAVTNIPIVLEGEVVGVYGIAKDITQRQRAEEALRESEIRTRAIVDT
                     AQDAIITMSSDGVIRSFNPGAERTFGYGAEEVVGEPLRMLMPERFHGSHEAGFRRYLS
                     GGEARVVGKGPVELAGRRKSGEEFPLELSLGEAHLGDERVFTGVIRDITARKRAEKTI
                     RESEQRFKQLFEQSVDTLLVHDSRGRIVDCNEEACRSLGYTRDEMLSLRVGDFATNLV
                     ARDERWKQEESTLWERALSGEPGRIAGVHRGMHRRRDGTTFPVEVHVGSVDYGGERML
                     FASARDITERLEAERELREAEERFRSAFESAAIGMALTDPGSGRYLRVNRALCEMFGR
                     SEEEMLATNFRDVTCPEDRDLTMDYERRVMAGELDSYQHEKRYLHGDGHLVWAQTNIS
                     VVRDSEGKPLHFVAQIQDITERRRAEESLRRSEANLAASQERAHLGSWEWDTATGGLL
                     WSDEHYRIFGHAPQSFEPDYDTHYLGAIHPEDRDLVCKAIDESFEGKPYNLDYRIVRT
                     DGETRHVQSSGEVLFDESGAPIGMSGTVQDITERKRAERQVFESEERYRLVSRATNEV
                     IWDNDFESGTQHWDGAIEAMFGYSADEVGESGAWWERRIHPDDRENVLSSLQEALDGG
                     GESWSCEYRLRRADGEYATVADRGYVVRDAGGRPVRMLGSMMDVTERKRAEEAQRQSE
                     ERFRGLADATFEGIAILERGWVLETNRAFADLFGYEQREILGKRVFDFVAPESRDLAR
                     RNIESGLEEPYEAVAISRDGARFDVEIRGKTSIYRDREVRVSAVRDITGRKQSERRLR
                     EAEERFRTLVEQMPAVTYRQATGTVDGLPSGAITYVSPQVEDQTGYPSQAFLEDPELW
                     LKIIHPEDREYVLAEDARTEECGETFEVEYRIICRDERVVWIRDEAVLIRDESGQPLY
                     WQGFQV"
BASE COUNT         1042 a         1802 c         2075 g          827 t
ORIGIN      
        1 aagatagagg ccgggcagat gaacatagag atcatagact tcgacctgcg gctcacggtc
       61 gaggacacgg tgacgcttct ggccgaccgg gctcatatca agggccttga gctggcgagc
      121 ttcgtggagc cggacatctc ctcccctctc cggggagatc ccggccgcat ccggcaggtg
      181 ctgacgaacc tccttggaaa cgcgatcaag ttcaccgagc agggcgaggt cgtcctgcgg
      241 gtcgagaccg ccggccggcg gcggggaaac cagcagagca tgcggaccgt tcgtttctcc
      301 gtctcggaca ccggcatcgg gatgacggag gagcagcagg aaaagatctt tgcctcgttc
      361 tcccaggccg acgcctccac gacgcgccgc tacgggggga cggggctggg gctcgccatc
      421 agcaggcaac tggtggagat aatgggcggg cgcatatggg tggagagcga gcccggcgcc
      481 ggctccactt tctctttcga gctgccgctg atggagacgg acggcggccc ggtaagagat
      541 acgtccggcg tggagctgtc ggggctcaag gcgctggtag tggacgacaa cgcgacgaac
      601 cgccagatcc tcgacaggca actctcctcg tggggcatgg cgagcgaggg tgtcgagggc
      661 ggcgcggagg ccctgcgcga gctgcgggag gccgccggac ggggcgagcc ttacgacgcc
      721 gcgatcctgg acatgcagat gccggggatg gacggtatgc gactcgcgcg cgagataaaa
      781 caggaccccg ccatctcccg cacccggctg atactcctca cctcgatggg ccggcgcggc
      841 gacggacggg aggccctgga ggcgggcatc gaggcctacc tcaccaagcc cgtgagacag
      901 tccgagctct acgacgccct cgcgaccctg atggcggcgg acagcaccgg ggacgaggat
      961 actgatagca gactggttac ccgccacacc ctgcgcgaga tgcggagcga gcgaggctcg
     1021 cgcgtgctgc tcgccgagga caacgacatc aaccagaagg tagcggtcag gatgcttgag
     1081 aggctcggat accgggtgga cgtggtcgaa aacggacgcg aggctctcga agcgctcgaa
     1141 cgcgaccctt cctacggcgc cgtgctgatg gacgttcaga tgccggagat ggacggctac
     1201 gaggcgaccg ccgagatccg ccgcctcgaa gcgctggagg cgtaccggct ccacgtccgg
     1261 ggcagctgcg cgggagcgcg cagcatcccg gtcatagcca tgaccgccaa cgccatgcag
     1321 ggcgaccggg agaaggctct cgccgccggc atggatgact acctctccaa gcccgtgaag
     1381 gcggaagacc tcaaagccgt actcggccgc tgggtgagcc cggaagcgcc cggcccggcg
     1441 gaggcggacg agcccgtaaa gatcgagagc ggaacggagc cgccgccgga gacgccgacg
     1501 gagtccccga atgagccgcc gctggatgac gcggtattat cggcgctgcg cgagttgcag
     1561 gaagagggag agccggacat catcgcggag ctggccgggc tgttcgtcga ggacaccgta
     1621 gagcgtctcg ggacgctgcg cgaggcgatc gaggccggag acgcggcggg cgtggagcgc
     1681 gccgcccacg ccctcaaggg cagctccggc aacatgggcg cccgcgggtt gagcgagcgg
     1741 tccgcccggc tggaggaggc cgggacggcg ggagccctct cggaagcgaa ggacctgctg
     1801 gagagcatcg agagcgagtt cgcccgtgtg aggctggccc tcgaagcgga agtccagcaa
     1861 gcggcgaata agcggtgatc ctgactcgag tggggaccgt gtgtatgatt ttcgaggggc
     1921 gaaactgata tgacgctgat ctgatggaga tgaccgggac atacgacctg cgcctcgtcg
     1981 cgctctcgat actggtggcg gtgatcgcct cctacgccgc tctggacctg gcggggcggg
     2041 tcacggcgtc gaagggcggc gcccgccggg tgtggctcgc cagcggcgcg gtggcgatgg
     2101 gagccgggat ctggtccatg cacttcatcg ggatgctcgc cttcagcgtg cccatgccga
     2161 tgacctacag cgcgcccacg gtcgcgctct cgctgctggt cgccatcgcc gcctcgggcc
     2221 tggcgctatt tatcgtgagc cggcggacta tggggcgtcc gcagctggtc tccggcggac
     2281 tcttcctggg cgcgggcata ctcgggatgc actacatcgg catggcgggg atgaggatgc
     2341 aagccgcggt cagctacgac cccctgctgg tcgggctctc ggcggtgatc gccttcggcg
     2401 cctccctcgc ggccctctgg ctcgcctacc agctccgccg cgaaagcggt ccggggggcc
     2461 ggtggctgcg ccttaaagcc gccagcgcgc tcgtgatggg cggggctatc tccgggatgc
     2521 actacacggg catggccgcc gcgagcttcg ccccgaccag ggacgtcctg aaggcgccgc
     2581 ccggcgccgc cgagaccggc acctccatcc tggccgccgc cgtgggcgcg gctacgctgg
     2641 ctatcctcgg ggtggcgatc ttcacctcgg tcctcaaccg gcgctttctg gcccaggcgg
     2701 cggcgctgga ggcatcggag cagcgctacg agtccctctt cgaggagaac ccggacgcgg
     2761 tcttctcgtt cgaccgggag ggcaggttca cctccgtcaa cccggcggtc gagcgcatca
     2821 ccggctaccc ggccgatgag atcctgggcc agacctccgg acgtctgctc gcccccgagg
     2881 acctggagcg cgctgcggaa cacttcatga agaccctgcg gggcgagccg cggagctacg
     2941 agacggcggt catctccagg gacggcagcc gcgtaccggt cgccgtcacc aacatcccaa
     3001 tagtccttga gggcgaggtg gtcggcgtct acggcatcgc caaggacatc acccagcgcc
     3061 agcgggcgga ggaggcgctc cgggagagcg agatccgcac ccgcgccata gtggacacgg
     3121 cgcaggacgc catcatcacg atgtcctccg acggcgtgat ccggtccttc aaccccggag
     3181 cggaacggac cttcggctac ggcgcggaag aggtcgttgg agagcccctg cggatgctca
     3241 tgcccgagcg tttccacgga tcgcacgagg cgggcttccg gcgctacctg tccggcggcg
     3301 aggcgcgcgt ggtcggtaaa gggccggtgg agctcgcggg acggcgaaag agcggcgagg
     3361 agtttccgct ggaactctcg ctcggagagg cgcatctggg ggacgagcgg gtgttcaccg
     3421 gtgttatacg cgacatcacc gcgcgcaagc gggcggagaa gaccatccgg gagagcgagc
     3481 agcgcttcaa gcagctcttc gagcagtccg tggataccct gctggtgcat gacagccggg
     3541 gccgtatcgt ggactgcaac gaggaggcgt gccggtctct gggctacacc cgcgatgaga
     3601 tgctctcgct ccgcgtcgga gacttcgcca cgaacctcgt cgcgcgcgac gagcgctgga
     3661 agcaggaaga gagcacgctc tgggagcgcg ccctctccgg agagcccggc agaatcgcgg
     3721 gggtccatcg cgggatgcac cgccgcaggg acgggacgac cttcccggtc gaggttcacg
     3781 tcggctccgt tgactacggc ggcgagcgga tgctcttcgc ctcggcgcgg gacatcaccg
     3841 aacgcctgga ggcggagagg gagctgcgcg aggccgagga gcggttccgg agcgccttcg
     3901 agagcgcggc catcggcatg gcgctcaccg atcccggtag cggacgctac ctgcgggtca
     3961 acagggccct gtgcgagatg ttcggccgct cggaggagga gatgctcgcc accaacttcc
     4021 gcgacgtgac ctgccccgaa gaccgggacc tcacgatgga ctacgagcgt cgtgtcatgg
     4081 cgggagagct agacagctac cagcacgaga aaagatacct ccacggcgac ggacacctgg
     4141 tatgggcgca gaccaacatc tcggtcgtca gggattcgga gggcaaaccg ctccacttcg
     4201 tagcccagat ccaggacata accgagcgcc ggcgggcgga ggagagcctg cgccgtagcg
     4261 aggcgaacct cgccgcatct caggagcggg cgcacctcgg gagctgggaa tgggacacgg
     4321 ccacgggtgg attgctctgg tcggacgaac actaccgcat cttcggccac gccccgcaat
     4381 ccttcgagcc cgactacgat acgcactacc tgggtgccat tcatcccgag gaccgggacc
     4441 tggtatgcaa agctatagat gaatccttcg agggaaaacc atataacctc gactaccgca
     4501 tagtgcggac agacggcgag acgcgccatg tccagtccag cggcgaggtc ttgttcgacg
     4561 agagcggcgc gcctatcggg atgtccggaa cggttcagga cataacggaa cgcaagcggg
     4621 ccgagcggca ggtcttcgag agcgaggagc gttaccggct ggtctcgcgg gccaccaacg
     4681 aggtgatctg ggacaacgac ttcgagagcg ggacacagca ctgggacggc gcgatcgagg
     4741 ccatgttcgg ctactcggcc gatgaggtcg gcgagagcgg cgcatggtgg gagagacgga
     4801 tccatccaga cgacagagag aatgtcttgt cgagcctgca agaagccctc gatggaggcg
     4861 gagagtcatg gtcctgcgag taccggctcc ggcgcgccga cggcgagtac gctacggtcg
     4921 cggaccgggg ctacgtggtg cgggacgccg gcggcaggcc ggtgcggatg ctcggctcga
     4981 tgatggacgt aacggaacgc aagcgggcgg aggaggccca gcgccagagc gaggagcgct
     5041 tccggggcct cgcggacgcg accttcgagg gcatagctat ccttgagcgg ggttgggtcc
     5101 tggagaccaa ccgcgcgttc gccgacctgt tcggttacga gcagcgggag atcctgggta
     5161 agagggtctt cgacttcgtg gcgccggaat cgcgggacct ggcgcggcgc aacatcgagt
     5221 ccggtctcga agagccttac gaggctgtcg cgatcagcag agacggagcc cggttcgacg
     5281 tggagatccg gggaaagaca tccatttaca gagaccgcga ggtgcgcgtg agcgccgtcc
     5341 gggacatcac cgggaggaag cagtcggaga ggcggctgcg ggaggccgag gagcgcttcc
     5401 ggacgctcgt cgagcagatg ccggccgtca cgtaccggca ggccaccgga actgtcgacg
     5461 gcctcccgtc cggcgcgatc acctacgtca gcccgcaggt ggaagaccag acgggctatc
     5521 cgtcgcaagc cttcctcgaa gacccggagc tctggctcaa gatcattcac cccgaggacc
     5581 gcgaatatgt gctggccgag gacgcccgca ccgaagagtg cggtgaaacc ttcgaggtcg
     5641 agtaccggat catctgccgc gacgagcggg tagtctggat ccgggacgag gcggtcctga
     5701 tccgggacga gagcggacaa cctctctact ggcagggctt ccaggt
//
LOCUS       DASSMB010000079         9068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_18127, whole genome shotgun sequence.
ACCESSION   DASSMB010000079 DASSMB010000000
VERSION     DASSMB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9068
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18127"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="VFJ72_05200"
     CDS             complement(<1..391)
                     /locus_tag="VFJ72_05200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7478897.1"
                     /translation="MVEWGAGTGRIAIPLAEAGHEVTAVEISESMVERGRKKSAAVEW
                     VRGDMRDAKLERKYGLAICAFNSFLCLVSVGDAPAFLRNAREHLEPGGLLGIEVSAFS
                     PEELLDPPGEPNLQHDFTRELPGGWLDR"
     gene            332..520
                     /locus_tag="VFJ72_05205"
     CDS             332..520
                     /locus_tag="VFJ72_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478898.1"
                     /translation="MARLGERYGDASGAGAPFDDGTGLASGEGVPEGQVVVVDVLQVI
                     EVGQRVVFLQEGLLGPVR"
     gene            565..1125
                     /locus_tag="VFJ72_05210"
     CDS             565..1125
                     /locus_tag="VFJ72_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET7478899.1"
                     /translation="MGTILIIVLVLIVVVLVIYAISTYNGLVRRRNEVEESYRQIDVQ
                     LKRRYDLIPNLLEGVKKYFRQEQDVLTQITQARSQAMQPQSPGQQAQSEARLSGAIGN
                     LFAVAENYPELRSNTVMLDFQEELSSTENKISFARQHYNDSVQGYNTKIQSFPAVIFA
                     RAMGFTEKEYFAAEGPERESVTVSYD"
     gene            1133..2083
                     /locus_tag="VFJ72_05215"
     CDS             1133..2083
                     /locus_tag="VFJ72_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HET7478900.1"
                     /translation="MDQGTFRDRIAVNRRNSLLLISGFLFFVVLFGYIVGYAWIGDPV
                     GALFGLALALVAGVVSGLISYYGGAGIMLAASRAKEITHDDAPVLFNVVEEMTIASGL
                     PMPKVYIIDDTAPNAFATGRDPEHAAVAVTKGLLDKLDRDELQGVIAHEMSHVGNFDI
                     RYSMLVGILVGTTVLISDFFLRGLWFGGGRGGRSREGGGQIQLIMMVIAILLAILAPI
                     FARLLQLSISRQREYLADATAVQMTRNPAALADALQKISGDREVLEAANRATAHLYIV
                     NPIKKFEKRSKGLFSTHPPIEERIKILRAIESGGIAQTSG"
     gene            2131..2201
                     /locus_tag="VFJ72_05220"
     tRNA            2131..2201
                     /locus_tag="VFJ72_05220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2163..2165,aa:Gln,seq:ctg)
     gene            complement(2172..3149)
                     /locus_tag="VFJ72_05225"
     CDS             complement(2172..3149)
                     /locus_tag="VFJ72_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET7478901.1"
                     /translation="MEETGTRRVAIACQGGGSHTAFTAGVLKKLLTEKRDRHEVVALS
                     GASGGAICALLSWYALLTDGAGRAVELLDSFWAENSANSYWDMVLNDLFVESSRLQGT
                     VALPEISPYLYPSMAQNRLRAMLEDHVEFERLADLAKPGSPELLVSAVDVLSGEFKVF
                     RDAEVSVEAILASAAIPNLFRAVRIGDGVYWDGLFSQNPPVREFVADRPVGKKPDEIW
                     VIQINPERREQEPTSVVDIMDRRNELSGNLSLNQEIRFVGKINNLLDHLPGDHYKRIT
                     VRRIEMLRPLDAASKLDRRPSFIQEMMAYGEEQAEDFLRELPDQDSNLD"
     gene            3321..3566
                     /locus_tag="VFJ72_05230"
     CDS             3321..3566
                     /locus_tag="VFJ72_05230"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET7478902.1"
                     /translation="MGALLVAMAAAWALYSGASADAESPSVYYEVSAGDTLWSVATAH
                     YPPSEDPRPIVEEIRALNDLSGSKLYPGMHLKLPSAG"
     gene            3702..4580
                     /locus_tag="VFJ72_05235"
     CDS             3702..4580
                     /locus_tag="VFJ72_05235"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7478903.1"
                     /translation="MREAFGCSSVRLHRSRLAWMGLSSLIAVLVGMFVLAGKSDAQAT
                     VPPFLAKEGTPPPGANIPCKPTRKHPAPVVLVHGTFETMEQNWAVLSPRLKQRGYCVF
                     ALNYGNRGLGPIQDSAKELRTFVNNVLAYTDARKVSLVGHSQGGMMPRYWIKNLGGKS
                     KVQDMIGLAPSNYGTELKQADFRRLLGIQSPCPACDQQRAGSTFLKNLNRGDDTPGPG
                     SFTQIATDDDEIIIPYTRCFLRGTERTRNITLQKYNGGLLVTHQNIYDDPFAQRLVFD
                     ALANPGPANPKRAFGL"
     gene            complement(4852..5691)
                     /gene="aac(3)"
                     /locus_tag="VFJ72_05240"
     CDS             complement(4852..5691)
                     /gene="aac(3)"
                     /locus_tag="VFJ72_05240"
                     /inference="COORDINATES: protein motif:HMM:NF033082.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3-N-acetyltransferase"
                     /protein_id="HET7478904.1"
                     /translation="MARSRLARDLRKLGVGPGGVMMVHCRMSALGHVAGGAETVVRAL
                     LDALGPEGTLMAYTGWQDEPPEDLGDLDDEARRVYLEEHPAYDPRVALSRREHGRIPE
                     ALRTWPGARHSWHPEAGVAAAGPLAEALTSEHPYDDAYGSGTPYARLVEFGGQVAVLG
                     APLDTVTLVHHAEAIAEVPGKRRVSYGMPVMAKTGERVWRTFSDIDTSEGALPYERVL
                     GEEDYIEHIARSALAAEASPGRGGPVGEATAYLFDARGLVEHAVGWIEHNFPSAGGST
                     DLE"
     gene            5873..6328
                     /locus_tag="VFJ72_05245"
     CDS             5873..6328
                     /locus_tag="VFJ72_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478905.1"
                     /translation="MYGPGRMVYAGRRRGSASPMLVVALVAGGILLFSAAGTFHFFPF
                     FLLFWMVPFLLAPMLRSSVRGVAGGVGDRARGPVDEGLKEKEILKVLARHGEVTPARA
                     ALETSLGVSEADRVLSKLAKDGHVEVRAHEGSLGYALWEHDRRELTDGS"
     gene            6363..6935
                     /locus_tag="VFJ72_05250"
     CDS             6363..6935
                     /locus_tag="VFJ72_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7478906.1"
                     /translation="MFGRGMKRGPFGPGPWGGFGPDPRQRGRMFEKGDLKYVILDLLA
                     EKPSHGYEVIRALEERFRGMYSPSPGSVYPTLQLLEDMGYTTATQQDGKKVYEITAEG
                     RKFLEENQRSVENIWTRVGGGWDPEIAAEMHEMRHEIMGLGKLFGREMHAGRLDREKL
                     RRIREVISGAARQVEEIVRERDDTGGTTSA"
     gene            7258..7647
                     /locus_tag="VFJ72_05255"
     CDS             7258..7647
                     /locus_tag="VFJ72_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7478907.1"
                     /translation="MIEGAAGCLAVYVRNIEEALRFYVSALGFERRGGREGRVEISLP
                     GARAGAGTWLRLVQPDTAGLPHSVIGGETGIIFRTQEIQETYEQLSERGVSFLCEPTL
                     RRWDLAEARFTDPDGNRFLLVKEWAAS"
     gene            7728..8486
                     /locus_tag="VFJ72_05260"
     CDS             7728..8486
                     /locus_tag="VFJ72_05260"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET7478908.1"
                     /translation="MRLKGLPRKFAPVLIAALLALTLAACGGGQEEPQGNAPEKTGQE
                     SGASATTEDTGASGGASEETTSGMEKTSEQASALQNAKIGPREITNMTPADGKPSDPP
                     QPLPKDPPKGVKLFPATTNETREGKIEYDRDPPTNGDHSPIWQNCGFYSKPIDNRTAV
                     HSMDHGVVWITYRPNLPAAQVQKLRSYGDEDYVIVSPYPGLPAPVIATAWRVQLRLDG
                     ADDPRLRQFVDQFRISELAPLSGNRCIGGVGEPE"
     gene            8555..9064
                     /locus_tag="VFJ72_05265"
     CDS             8555..9064
                     /locus_tag="VFJ72_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7478909.1"
                     /translation="MATDHSGEGILSEELTGKREEIIGLLKKAYFMELETVINYVTNS
                     TNPDGVRAQQVKESLIPDIQEELGHAQNFAARIKELYGVVPGSAEFVAEQSFLQPPED
                     QVDVVHVIKGVISAERGAIEHYTHLIEVTEGVDQVTQDMVIDILRDEQGHMRLFEGFL
                     KEYAAEGKA"
BASE COUNT         1714 a         2822 c         2919 g         1613 t
ORIGIN      
        1 aacggtcgag ccatccgccc ggcagttcgc gggtgaagtc gtgttgcagg ttcggttctc
       61 cgggcggatc gagcaactcc tccggcgaga aagcggagac ctctatcccg agcagccctc
      121 ccggctcaag gtgctcacgc gcgttgcgca ggaaagccgg cgcgtcgccc acactgacga
      181 gacacagaaa cgagttgaac gcgcagatgg cgagcccgta ctttcgttcc agcttcgcgt
      241 ctcgcatgtc gccccggacc cattcgacag ccgcgctctt ttttcggccc cgctcgacca
      301 tgctctctga gatttcgacg gcggtgactt cgtggcccgc ctcggcgagc ggtatggcga
      361 tgcgtccggt gccggcgccc cattcgacga tgggaccggg ctcgcgtccg gcgagggcgt
      421 gccagaaggg caggtcgtag tcgtggacgt actccaggtc atagaggtcg gccagagggt
      481 cgtattcttg caggagggtc tccttggtcc ggtccgttga ggctataata gccggtgaca
      541 gcccgcctag tcgtggaggt tctcttgggc actatcctga tcatagttct cgtcctgatc
      601 gtcgtcgtgc tcgtgatcta cgccatctcc acctacaacg ggctggtgcg gcgtcgcaac
      661 gaggtggagg aatcgtaccg gcagatagac gtgcaactca agcggcgcta cgacctgatc
      721 ccgaacctcc tcgaaggcgt gaagaagtac ttccgccagg agcaggacgt gctgacccag
      781 atcacgcagg cccgctcgca ggcgatgcag ccgcaatcac ccggacagca ggcgcagtcg
      841 gaggcgcggc tctccggcgc catcgggaac ctgttcgccg tcgcggagaa ctacccggag
      901 ttgcggtcga acacggtgat gctggacttc caggaggagc tgtcctcgac ggagaacaag
      961 atctccttcg cccgccagca ctacaacgac tccgtgcaag gctacaacac gaagatacag
     1021 agtttcccgg ccgtcatctt cgcccgcgcg atgggtttca ccgagaagga gtacttcgcg
     1081 gcggagggac cggagcggga gtcggtaacg gtctcctacg actgaggaac ggatggacca
     1141 gggcactttc cgggaccgca tcgccgtcaa ccggcgcaac agcctgctcc tgatctcggg
     1201 cttcctcttc ttcgtggtcc tcttcggcta tatcgtcggg tatgcctgga tcggggaccc
     1261 ggtcggcgcc ctcttcggtc tcgccctcgc cctcgtcgcc ggcgtagtct ccggcctgat
     1321 ctcctactat ggcggcgcgg ggataatgct cgccgcgtcg cgggccaagg agatcacgca
     1381 cgacgacgca cccgtcctct tcaacgtggt cgaggagatg acgatagcct ccggcctccc
     1441 gatgccaaag gtctacatca tagacgacac cgctcccaac gccttcgcca ccggccgcga
     1501 cccggagcac gccgccgtcg ccgtgacgaa gggtctcctc gacaagctcg accgcgacga
     1561 gcttcagggc gtcatcgcgc acgagatgtc ccacgtcggg aacttcgaca tccgctactc
     1621 catgctcgtc ggcatcctgg tcgggactac ggtcctcatc tcggacttct tcctgcgcgg
     1681 gctgtggttc gggggcggca ggggaggccg cagccgcgag ggtggaggtc agatacaact
     1741 gatcatgatg gtcatagcaa tccttctagc catcctcgcc ccgatcttcg cccgcctcct
     1801 gcaactttcc attagccgcc agcgcgagta cctcgccgac gcgaccgccg tgcagatgac
     1861 ccgcaacccc gccgccctcg ccgacgccct acagaagatc tccggcgacc gcgaggtcct
     1921 cgaagccgcc aaccgcgcca ccgcgcacct atacatcgtc aaccccatca agaagttcga
     1981 gaagcgctcg aagggcctct tctccaccca cccgccgata gaggagcgca taaagatact
     2041 gcgcgccata gagagcggcg gaatagctca gaccagcggc tagaagctcg acgccgggcg
     2101 gataagaggt tacaatactc gccgttgcat tgccggatgg tgtaacggca gcacgagtga
     2161 ctctgaatca cttagtccag gttcgaatcc tggtccggca gctccctcag gaagtcctcg
     2221 gcctgctcct cgccgtaggc catcatctcc tgtatgaacg atgggcggcg gtcgagcttg
     2281 gaggcggcgt ccagggggcg gagcatctcg atcctgcgca ccgtgatgcg tttgtaatgg
     2341 tcgccgggaa gatggtctaa gaggttattg atcttcccca cgaaccgtat ctcctggttt
     2401 agcgagaggt tgcccgagag ctcgttgcgc cggtccatta tgtccaccac ggaggtcggc
     2461 tcctgctccc gcctctccgg gtttatctgg atcacccaga tctcatcggg cttcttcccc
     2521 accggacgat ccgccacgaa ctcccttact ggagggttct gcgagaagag accgtcccag
     2581 tacactccgt caccgatcct cacggcccga aacaggttcg ggatggcggc ggaagccagg
     2641 atcgcctcga cgctcacctc ggcgtctctg aagactttga actctcctga aaggacgtcc
     2701 acggcgctga ctagcaactc cgggctgccg ggtttcgcga ggtccgccag cctctcgaac
     2761 tcgacgtggt cttcgagcat cgcgcgcagg cggttctggg ccatggaggg gtagaggtac
     2821 gggctgatct cgggcagggc gacggtcccc tgcagccggc tggactcgac gaacaggtcg
     2881 ttgagcacca tgtcccagta cgagttggcc gagttctccg cccagaacga gtccaacaac
     2941 tctacggctc tccctgcccc gtccgtgagc agcgcatacc aggacagcag cgcgcagata
     3001 gcgccgcccg aggctccgct caacgcaacg acctcgtgcc tgtctcgctt ctccgtcagc
     3061 agtttcttga gtaccccggc tgtgaaagcc gtgtggctac cgcctccctg gcaggctatt
     3121 gccacccgcc gcgtcccggt ctcctccatg atcacctcct ctctccgata ctacacgaga
     3181 actggcagat tgatgctgcc gttgggcctt cttccgcgcc gggaaataat ctctgtctgg
     3241 gtcaataaac cgttgggaac ggacctcgta tccagtatct tccacgggtg agaccgcacc
     3301 gcacatatcg gcgccggaga ttgggcgcgc tactcgtcgc gatggccgcc gcctgggctc
     3361 tgtactccgg cgccagcgcc gacgccgagt cgccgtccgt gtactatgag gtctcggcgg
     3421 gcgatactct ctggtccgtg gcgaccgcac actaccctcc ctctgaagat ccacgaccga
     3481 tagtcgagga gatccgggcg ttgaacgacc tttcggggag caagctctac cccggcatgc
     3541 acctcaaact gccctcagcc ggttgagcac cggcctgccg cggacaaggc gttgcccact
     3601 cgcgtagcgt gttccgaagc ctgtttacat cgtcgtgacg gacaacgata atggcgccgt
     3661 ctaatggaga tctttttgcg tgaaggtaga gaggcgggaa catgagagag gctttcggat
     3721 gtagttcggt caggcttcat agatccaggc tcgcctggat gggtctctct tcactgatcg
     3781 ccgtccttgt gggcatgttc gtgctcgccg ggaagtcgga cgctcaggct accgttccgc
     3841 ctttcctggc caaggaaggc acgccgccgc ccggagcgaa cattccctgc aagccgacca
     3901 ggaaacatcc cgctccggtg gtgctcgtgc atgggacgtt cgagactatg gagcagaact
     3961 gggccgttct atctccccgg ctgaagcagc ggggctactg cgtgttcgcg ctcaactacg
     4021 gcaaccgcgg gctcggacct atccaggact ccgcgaaaga gcttaggacc ttcgtaaaca
     4081 acgtgctggc atacactgat gcccgcaaag tctcgctcgt cggacacagc cagggcggga
     4141 tgatgccccg gtactggatc aagaacctcg gcggcaagag caaggtgcag gacatgatcg
     4201 ggctggctcc ctccaattac ggtacggagc tcaaacaggc cgacttccga aggctcctgg
     4261 gtattcagag tccgtgtcct gcctgcgacc agcagcgggc cggctccacg ttcctgaaga
     4321 acctcaaccg cggcgatgac actcccggcc ccggctcctt cactcagatc gccacggacg
     4381 acgatgagat cataatcccc tacactcggt gcttcctgcg tggcacggag cgcaccagaa
     4441 acataacgct ccagaaatac aacggcggac tcctcgtaac ccatcagaac atctacgacg
     4501 atccgttcgc ccagcgtctc gtcttcgacg ccctcgccaa ccccggaccc gcaaacccaa
     4561 agcgcgcctt cggactttag taaccaggcg gaacgtccag gccgcgatcg cgggatcttt
     4621 gccagcgctg gtgggaccgt atggtgtgga cacgccccgt tacaaccgca tgagcgagca
     4681 gtggttaacc gggcttgtgt atacgttctc tggacctgct tcagatgttt gtcagccggc
     4741 ttttcgaagc ctgctcgccc ctgatgcgct ggaatgaaga cgtgccgttg gagcgtcgcc
     4801 gttattgccg tctcctacgg tgagagtaga accgaagggt aggaacctcg gctattcaag
     4861 gtccgtcgag cccccggcgg acgggaagtt atgttcgatc caaccgaccg cgtgctcgac
     4921 cagtccgcgg gcgtcgaaca ggtaggccgt agcctctccg accggccctc ccctccccgg
     4981 cgaagcctcg gccgcgagcg ccgaacgcgc gatgtgctcg atgtagtcct cctcaccgag
     5041 gacgcgctcg taaggcaagg ctccctccga ggtgtcgatg tccgagaagg tccgccacac
     5101 gcgctcgccg gtcttcgcca tcacgggcat gccgtagcta acgcggcgtt tgccggggac
     5161 ctctgctatg gcttccgcat gatgcacgag cgtcacggtg tcgagcggcg cgcccagcac
     5221 tgcgacctgg ccgccgaact cgaccagccg ggcgtagggc gtgccggagc cgtaagcatc
     5281 atcgtaaggg tgctcggagg tgagggcctc ggcgaggggg ccggcggccg ccacacccgc
     5341 ctccggatgc cagctgtggc gcgctccggg ccaggtcctc aaggcttcgg ggatcctccc
     5401 gtgttcgcgg cgggagagag cgacgcgcgg gtcataagcc ggatgttctt cgaggtagac
     5461 acgcctcgcc tcatcgtcca gatcgccgag gtcttcaggc ggctcgtcct gccagccggt
     5521 gtaggccatg agcgtcccct cgggacccag agcgtccagc agggcgcgta cgacggtctc
     5581 cgcgccaccg gccacatggc cgagcgcgga catccggcaa tggaccatca tcaccccacc
     5641 cggccccacc ccgagcttcc tcaggtcccg agccagcctt gaacgcgcca ctaacacatc
     5701 ccacacggta gcaggagcgc cggaggaaga ctatggggag agcatgacga ggaggtctga
     5761 cctatcctcg aactgggcac ccaaatgccg ccagaaattc caggtactat cgcgatatat
     5821 cgtgatatac tgcgatctga agtcgagcgg tggatgtagt gaaggaggcg agatgtacgg
     5881 tccgggcagg atggtttacg cgggcaggcg gcgggggtct gcgtcgccga tgctggtggt
     5941 ggctctggtg gctggtggaa tccttctctt ctctgcggcg ggtacgttcc acttcttccc
     6001 gttcttctta ctcttctgga tggttccgtt tctcctggcg ccgatgctga ggtcatcggt
     6061 gcggggagtt gccggaggtg taggggatcg ggctaggggg ccggtcgatg aggggctcaa
     6121 ggagaaggag atcctgaagg tgctggcacg gcatggcgag gttacaccgg cgcgggcggc
     6181 gcttgagacc tcgctgggcg tttcggaggc ggaccgggtg ctctcgaagc tggctaagga
     6241 cgggcatgtg gaggtgcgcg ctcacgaggg gagcctgggg tatgcgctgt gggagcacga
     6301 ccggagggag ttgacggatg ggtcatagag ttgcgaggcg attgcacaag gaggcgcgaa
     6361 gtatgtttgg gcgagggatg aagcgaggac cgttcgggcc gggaccgtgg ggcgggtttg
     6421 ggccggatcc caggcagcgg ggacggatgt tcgagaaagg ggacctcaag tacgtaattc
     6481 tggacctgct cgcggagaag ccctctcacg ggtacgaggt catccgggct ctggaggaga
     6541 ggttccgcgg gatgtactcg ccgagccccg gttcagtcta tcccacgctg cagttgttgg
     6601 aagacatggg ttacacgacc gcgacgcagc aggacggcaa aaaagtgtac gagatcaccg
     6661 ccgagggacg caagttcctc gaagagaatc agcggtcggt ggaaaacatc tggacccggg
     6721 tcgggggcgg atgggacccc gagatcgcgg ccgagatgca cgagatgcgg cacgagatca
     6781 tgggcctcgg caagctcttc gggcgcgaga tgcacgccgg acggctggac cgggagaagc
     6841 tgcgccgcat ccgggaggta atctccggcg ccgcaaggca ggtcgaggag atcgtgcgcg
     6901 agcgagacga cacgggaggg accaccagcg cctagttcga gcccgagcga tgaggcaatg
     6961 agcggattcc ttcaagatga cgccccggat acacaccacg aagccgcgcc gcactggcgc
     7021 ggtttctgct gtgcggttct gatcctcccg ccttcgatgt agctggcgct cttacgaacc
     7081 ggtggcattg gaagagagga tggccgaagg cgggagcttt tcaaggaata ttcaccgaga
     7141 ttttacatcc ggctaacaga gagaacgccg ggagctatta gcatattggg gtccggctga
     7201 tgcgccgtct gcgcgtaccg gcgggagaga ggcgaagaaa atacggaagg aggcggtgtg
     7261 atcgaaggcg cagccggatg tctggccgtg tacgttagga atatcgagga ggcgctccgt
     7321 ttctacgtga gcgctctggg gttcgagcgg cgcggcgggc gagaggggcg agtggagatc
     7381 tcgcttccag gagccagggc tggagccgga acctggttga gactcgtgca gccggatacg
     7441 gcggggttgc ctcacagcgt gatcggcggc gagacgggga tcatcttccg tacgcaggag
     7501 attcaggaga cctacgagca gctttcggag cggggcgtga gcttcctgtg cgagccgacc
     7561 ctgcgccgct gggacctcgc cgaggcccgg ttcaccgacc cggacggcaa ccgattcctg
     7621 ctcgtcaagg agtgggcggc gagctagttt cgccaccggc gatagatcta tagtctccgc
     7681 cacatccgta gtatcctgag aaacggttct cgaaggaggc tgcggaattg cgtttgaaag
     7741 gtctgccgcg aaagtttgct cccgtactca tagccgcttt gctggcgctc accctggccg
     7801 cgtgcggtgg cggacaggag gagccgcagg gtaacgcgcc ggagaagacg ggccaggagt
     7861 ccggggcttc cgcgacgacg gaggacaccg gtgcgtccgg cggagcttcc gaagagacga
     7921 cctccgggat ggagaagacc agcgagcagg caagcgcgtt gcagaacgcg aagatcgggc
     7981 cgagggagat cacgaacatg accccggctg acgggaagcc ttcggacccg ccgcaaccgc
     8041 tgccgaagga cccgccgaaa ggtgtcaaac ttttcccggc cacgacgaac gagacgcggg
     8101 aggggaagat cgagtacgat cgcgatcccc cgaccaacgg agaccactcc ccgatctggc
     8161 agaactgcgg gttctactcg aagcccatag acaacaggac agccgtccac agcatggacc
     8221 acggggtggt ctggatcacg taccgtccca acctgcccgc tgctcaggtc cagaagctcc
     8281 gttcttacgg cgacgaggac tacgtcatcg tgagcccgta tcctggactg cccgcgcccg
     8341 tgatcgctac cgcctggcgg gtgcagctac ggctcgacgg cgcggatgac ccgcgcctgc
     8401 ggcagttcgt ggatcagttc aggatcagtg agttggcgcc gctctccggt aaccgctgca
     8461 tcggcggcgt gggcgagccg gagtagccgc gttttacacg gccgccgggg tctgtgagaa
     8521 tgctcccacc atcatctaca gagggagaag gggaatggcg accgatcaca gcggcgaggg
     8581 gatactctcc gaggaactga ccgggaaacg cgaggagatc atcggtctcc tgaagaaggc
     8641 gtacttcatg gagcttgaga cggtcatcaa ctacgtcacg aattcaacca accccgacgg
     8701 cgtccgcgcc cagcaggtga aggagtccct gatcccggac attcaggagg agttggggca
     8761 cgcccagaac ttcgcggcgc gcataaagga gttgtacggc gtcgtccccg gttcggcgga
     8821 gttcgtggcg gagcagagct tcttgcagcc gccggaggat caggtagacg tagttcacgt
     8881 cataaagggc gtgatctcgg ccgagcgcgg cgccatcgag cactacaccc acctcatcga
     8941 ggtcacggag ggcgtggatc aggtaacgca ggacatggtc atagacatcc tgcgcgacga
     9001 gcagggccac atgcgcctct tcgaaggctt cctgaaggag tacgccgccg agggcaaagc
     9061 ctaaccta
//
LOCUS       DASSMB010000080         4779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_271372, whole genome shotgun sequence.
ACCESSION   DASSMB010000080 DASSMB010000000
VERSION     DASSMB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4779
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271372"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VFJ72_05270"
     CDS             complement(<1..845)
                     /locus_tag="VFJ72_05270"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7478910.1"
                     /translation="MDLSKILEFPIKEHFAQPRALMGAGAHENAGPEAAEMGLKHVLF
                     VTSGLKGTGIIDECKTNFENAGVSVTVYDKVESNPKDYNVMDAYKAFTEAECDGFVSI
                     GGGSSHDTTKGARVVAAHDGRNTNEFQGLNASEKLDNPAHIAINTTVGTGSETTPAYV
                     ITDTTSPKAPHKWVAFDRASTTTLAINDPVLMMTQPPQFVAYTGFDTMAHASECYTNR
                     VQFHSATPQALQAVKLTTENLREATYNPHNFKAMEAMCWAQYMAAQAFSSGLLGILHS
                     LSHAV"
     gene            1018..1482
                     /locus_tag="VFJ72_05275"
     CDS             1018..1482
                     /locus_tag="VFJ72_05275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7478911.1"
                     /translation="MEDARERTVTRESLEVRSRFPHFQQIPTRWMDNDVYGHVNNVVY
                     YSYFDTVVNEYLIREGVLDIERSPVIGLVVETQCRYFEPVTFPDTVHAGLRVARLGRS
                     SVRCEIGLFRNDSETAAAQGHFVHVYVERETRKTSALPEEMRAALERIAVSG"
     gene            1839..2303
                     /locus_tag="VFJ72_05280"
     CDS             1839..2303
                     /locus_tag="VFJ72_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7478912.1"
                     /translation="MRRIQMTVDGKPHVADVEPRLLLIHHLRENLGIGGAHVGCDTSN
                     CGACTVLMDGESIKSCTVLAVQADGREITTVQGIAENGEWHPMQTAFHEQHGLQCGYC
                     TPGMIMASISLLKENPNPSEHEIREALEGNVCRCTGYENIIKAVQQVAGQAS"
     gene            2320..4758
                     /locus_tag="VFJ72_05285"
     CDS             2320..4758
                     /locus_tag="VFJ72_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7478913.1"
                     /translation="MTQAPERYMGGGVPRKEDPALITGKANWTDNIKLPGMLHIAFVR
                     SPFAHAKVTHLDVSGALEQPGVVAAYTGADLADEWPGGVPCGAQVAEDMKSPFFPPIV
                     TDEVNQLGDVVAVVVATDRYTATDALEFVEVDYEPLDVVVDMEEAIKEGAPLVHEDLG
                     TNACFNAVMANGDVDAAFEKADVVVKERYIQQRVLPTPIEPRATVVYQEPGRQGYVMY
                     TATQVPHLVKVVLSLSTGVPENEIRIVAPDVGGGFGAKLNLYREEIVTFVLSRKLGVP
                     IKWNEARSENYLGMIHGRDQIQDIELAATSDGKLLGMRVKLLDDFGAYLQLLTPLIGL
                     GGVGMFPGVYDFEAFSGEITGVFTNKTPTDAYRGAGRPEAAYAIERAMDSLARKLNMD
                     PAQLRRKNFIGPSEGAITNPAGLEYDSMNLEPALDKLLELADYEGLRREQQQRRESND
                     PVQLGIGLSTYTEICGFGPSQALAGLGLGGGGWEMSNVRMLVTGKVEVATGTSPHGQG
                     HETSWSQIAADALGVTPDDVVVIHGDTASVPFGRDTYGSRSAPVGGVAVWQAANKVVE
                     KAKKLAAHMLEAAEGDIEFEGGRFSVAGSPDQSVTIQEVAGQSYLGADLPEGMEPTLS
                     ESVVFDPPNFTFPFGAHLCVTEVDTETGKVKVRDFFAVDDCGPIINPTIAAGQLHGGI
                     AQGLAQALYEEAVYDESGNLVTGSMVDYLIPGAPELPNYTLEHTVTPSPTNPLGVKGI
                     GEAGTIGSTPAAINSVIDALSHLGVKHIDMPALPFNVWSTIQEARGRSAGTRTGDSSV
                     AQATDNTTPGGESL"
BASE COUNT          876 a         1477 c         1611 g          815 t
ORIGIN      
        1 cagaccgcgt gcgagagcga gtgcaggatg cccagaaggc cggagctaaa cgcctgcgcg
       61 gccatgtact gcgcccagca catcgcctcc atcgccttga agttgtgcgg gttgtaggtc
      121 gcctcgcgca ggttctccgt cgtcagcttg acggcctgca aagcctgcgg tgtcgcggag
      181 tggaactgga ccctgttcgt gtagcactcc gaggcgtgag ccatcgtgtc gaagcccgtg
      241 taggctacga attgcggcgg ctgggtcatc ataagcaccg ggtcgttgat cgccagcgtc
      301 gtggtcgagg cccggtcgaa agccacccac ttgtgcgggg ccttcggcga ggtcgtgtcc
      361 gtgatgacgt aggccggcgt tgtctccgag cccgtcccga ccgtagtgtt gatcgcgatg
      421 tgcgccgggt tgtcgagctt ctcggaggcg ttcaggccct ggaactcgtt ggtgttgcgg
      481 ccgtcgtgag ccgccacgac ccgcgcgccc ttggtggtgt cgtgcgagga gccgccgccg
      541 atggagacga agccgtcgca ctcggcctcg gtgaacgcct tgtaagcgtc catgacgttg
      601 tagtccttcg ggttcgattc gaccttatcg tagactgtca ccgagacacc cgcgttctcg
      661 aagttagtct tgcactcgtc gatgatcccg gtccccttca ggcccgaggt gacgaagagc
      721 acgtgcttga ggcccatctc cgccgcctcc ggcccggcgt tctcgtgggc gcccgcgccc
      781 atgagcgccc tcggctgcgc gaaatgctcc ttgatcggaa actcaagaat cttcgagaga
      841 tccattaagt aatcgcctcc ttttcctttt ccgctcaaca caaaaaacga ccgaatggct
      901 actgatgcgc ttcgcctttt tactcctttc tccattccct ttgagagagt agtcactatc
      961 ctatctcccg gaacctgtgt caaactgttt ggatggatcc agagccgaga ggggaagatg
     1021 gaggacgcgc gagagcggac ggtgacaaga gaatctctag aggtccggag ccggtttccg
     1081 cacttccagc agataccgac gcgctggatg gacaacgacg tgtacggaca cgtcaacaac
     1141 gtcgtctact attcgtactt cgacacggtg gtgaacgagt acctgatccg tgaaggcgtc
     1201 ctggatatcg agaggagccc ggtcataggg ctcgtcgtcg agacgcaatg ccggtacttc
     1261 gagccggtta ctttccccga caccgtccat gccgggctgc gcgtagcccg cctgggccgc
     1321 agcagcgtac ggtgcgagat cgggctgttc aggaacgata gtgagacggc cgccgcccag
     1381 ggccatttcg tccacgtcta cgtcgaacgc gagacccgca agacatccgc cctgccggag
     1441 gagatgcgcg ccgcgctgga acggatagcg gtcagcggct agtttttctc gcgggtgtgc
     1501 ctcatgcacc agcctcgtgt tcagatccat tgcagcgcgg gtttttagca ttcatgtggc
     1561 cagcataccg atggtggtag atcatagtgg tgggtggagt ggagatcaga gggttgtgtg
     1621 aacaaacgaa tgagtagagc gagaattccg ggcgtgatag actgcctcgg cttcactatg
     1681 atagggggta acgaataggg gtatttgtac cgggtgaggc gcggggtgtg ttgtctccca
     1741 cggcggtgga ggttgccggg gtgcattcct gcgcgcaggg cacattgtaa cggggaacgg
     1801 cgtccttttt agggcgttac tagggaaagg agcgaggttt gcgtaggatc cagatgacgg
     1861 tggacgggaa gccgcacgtc gcggacgtag agccgaggct actcctgatc catcacctgc
     1921 gggagaacct ggggatcggg ggggcgcacg tcggatgcga cacctcgaac tgcggggcct
     1981 gcacggtgct gatggacggc gagtcgatca agtcctgcac ggtactggcg gtgcaggcgg
     2041 acgggcgtga gatcacgacg gtccagggca tcgccgagaa cggtgagtgg cacccgatgc
     2101 agacggcctt ccacgagcag cacggcctgc agtgcggcta ctgcacgccg ggcatgatca
     2161 tggcctccat cagccttttg aaggagaacc cgaacccgtc ggagcacgag atccgggaag
     2221 ccctcgaagg taacgtatgc cgctgcaccg gctacgagaa catcatcaag gccgtccagc
     2281 aggtagccgg acaggcgagc tagaaaggga ggcgttggag tgactcaagc accagaacgc
     2341 tacatggggg gcggggtacc ccgcaaggag gacccggccc tcataaccgg caaggccaac
     2401 tggaccgaca acatcaagct gccggggatg ctgcacatcg cgttcgtgcg tagcccgttc
     2461 gcgcacgcca aggtgacgca cctcgacgtc tcgggggctc tggagcaacc cggcgtggtc
     2521 gcggcctaca ccggcgcgga tctggcggac gagtggccgg gcggcgtgcc gtgcggcgcg
     2581 caggtggccg aggatatgaa gagcccgttc ttcccgccca tcgtcacgga cgaggtgaac
     2641 caactcggcg acgtggtggc ggtggtggtc gccaccgacc gctacacggc taccgacgcc
     2701 ctggagttcg tcgaggtgga ctacgagccg ctggacgtgg tcgtggacat ggaagaggcc
     2761 atcaaggagg gcgccccgct cgtccacgaa gacctgggca ccaacgcgtg cttcaacgcg
     2821 gtgatggcca acggggacgt ggacgccgcc ttcgagaagg ccgatgtggt ggtaaaggag
     2881 cgctacattc agcagcgtgt cctcccgacg cccatcgagc cgcgggcgac ggtcgtctac
     2941 caggagcccg gacgccaggg gtacgtcatg tacacggcga cccaggtgcc acacctggtc
     3001 aaggtcgtgc tttcgttgtc caccggggtt ccggagaacg agatccggat cgtcgccccc
     3061 gacgtgggcg ggggattcgg cgccaagctg aacctgtacc gcgaggagat cgtgaccttc
     3121 gtcctgtcca ggaagctggg cgtgcccatc aagtggaacg aagcccgctc ggagaactac
     3181 ctgggcatga tccacgggcg cgaccagatc caggacatcg aactcgccgc cacgagcgac
     3241 ggtaagctcc tgggcatgag ggtgaagctg ctcgacgact tcggggctta cctgcagctc
     3301 ctgaccccgc tcatcgggct cggcggggtc ggcatgttcc ccggcgtcta cgactttgag
     3361 gcgttctccg gagagatcac gggcgtcttt accaacaaga ccccgaccga cgcctaccgc
     3421 ggcgcgggcc gccccgaggc cgcctacgcc atcgagaggg ccatggactc cctggcccgc
     3481 aagctgaaca tggacccggc ccagttgcgg cgcaagaact ttatcgggcc gtccgagggg
     3541 gcgataacga acccggccgg tctcgagtac gactcgatga acctggagcc ggcgctggac
     3601 aagctcctgg agctggcgga ctacgagggg ttgcgccgcg agcagcagca gcggcgcgag
     3661 agcaacgacc ccgtgcagct cggcataggg ctctcgacgt acacggagat ctgcggcttc
     3721 ggaccctcgc aggcgctcgc cgggctcggt ctcgggggcg gcggctggga gatgtccaac
     3781 gtgcgcatgc tcgtgacggg caaggtcgag gtcgcgaccg gcacctcgcc gcacggccag
     3841 ggccacgaga cgagctggtc ccagatcgcc gccgacgccc tgggcgtcac ccccgacgac
     3901 gtggtggtca tccacggcga cacggcgtcc gtccccttcg ggcgcgacac ctacgggtcc
     3961 aggagcgcgc cggtgggcgg cgtggcggtt tggcaggccg ccaacaaggt cgtggaaaag
     4021 gccaagaagc tcgcggcgca catgctggag gccgccgagg gtgacatcga gttcgagggc
     4081 ggcaggttct ccgtggccgg ctctccagac cagagcgtca cgatccagga ggtcgccggt
     4141 cagtcgtacc tgggcgccga cctgccggag gggatggagc ctaccttgag cgagagcgta
     4201 gtcttcgacc cgccgaactt caccttcccg ttcggcgctc acctctgcgt cacggaggtg
     4261 gacaccgaga ccggcaaggt caaggtgcgc gactttttcg cggtggacga ctgcggcccg
     4321 atcatcaacc cgactatcgc ggcggggcag ttgcacggcg ggatcgccca gggcctcgcc
     4381 caggccctgt acgaggaggc cgtctacgac gagagcggca acctggtaac cggctcgatg
     4441 gtggactacc tgatccccgg cgcccccgag cttcccaact acaccctcga gcatacggtc
     4501 acgccctcgc cgaccaaccc gctcggggtc aaggggatcg gggaagccgg gacgataggc
     4561 tcgacgcccg cggccataaa ctccgtcatc gacgcgctct cgcacctggg ggtgaagcac
     4621 atagacatgc ccgccctgcc gttcaatgtg tggagcacga tccaggaggc ccggggtcgc
     4681 tccgccggca cgcgcacggg cgattcctcc gtcgcgcagg caaccgacaa cactactccg
     4741 ggaggtgaat ccctgtgatc cccgtagcct ttgactacg
//
LOCUS       DASSMB010000081         6173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1539322, whole genome shotgun sequence.
ACCESSION   DASSMB010000081 DASSMB010000000
VERSION     DASSMB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539322"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="dhaK"
                     /locus_tag="VFJ72_05290"
     CDS             complement(<1..982)
                     /gene="dhaK"
                     /locus_tag="VFJ72_05290"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HET7478914.1"
                     /translation="MKKLINAPEKVVEEMLRGMELAHGGLIRVIPEHSIVVRADAPVQ
                     GKVALISGGGSGHEPTHGGYVGPGMLDAACAGAVFTSPTPDQMLEATKAVDGGAGVFY
                     VVKNYTGDVLNFEMAGELAEAEGIQTDYVVVNDDVAVEDSTYTSGRRGVAGTIFVHKI
                     CGAAADDGKDLAGIKELAEKVNANVRSMGMALTSCTPPEAGEPIFQIGDDEMEIGMGI
                     HGEPGTERKKIEPANDIVDEMLGKILGDNVDFSGSDVAVMVNGMGGTPIMELYVAYSH
                     IADVLKNEGVNVFRMPYVGDYMTSLEMAGFSITLLKLDEEMKGYLTAPCDV"
     gene            complement(1050..2573)
                     /gene="glpK"
                     /locus_tag="VFJ72_05295"
     CDS             complement(1050..2573)
                     /gene="glpK"
                     /locus_tag="VFJ72_05295"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET7478915.1"
                     /translation="MAGEYILAIDQGTTSSRAIVFNHDSATVGEAQQEFTQHYPKPGW
                     VEHDANEIWEVTLGVMRKALDDAGVSPDQIAAIGITNQRETVAVWDRETGEPVHNAIV
                     WQDRRGAQICTELSEAGNDEMVANKTGLVIDAYFSGSKIKWMLDNIDGLKERAQNGEL
                     ALGTMDTWLIWKLTGGREHVTDYSNASRTLMYNIFDLKWDDELLEMLGGVPREMLPEV
                     KPSSFVYGNTDAEVFGAEVPVAGVAGDQQAALFGEVAFEKGLTKNTYGTGSFALMNTG
                     TEPIKSERQMLTTIAWGLGDEPVEYALEGAIFITGAAVQWLRDGLGIIKDASETEELA
                     QSLDSNDDVYFVPGLVGLGAPHWDSYARGTIVGLTRGTTRAHLARAALESMCYQTRDA
                     VEAMELDSGIDIPTFKADGGAAANRWTMQFQADILGVPVEVPETLETTALGAAYLAGL
                     AVGFWSDKEELKSRWRLRRRYEPRMDEEERERLYGRWQEAVKRAKNWAQEAVESPLT"
     gene            complement(2796..3383)
                     /locus_tag="VFJ72_05300"
     CDS             complement(2796..3383)
                     /locus_tag="VFJ72_05300"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HET7478916.1"
                     /translation="MENTARSKADRFFDALRANPVIPAVRGPDGALDHALETQPAAIF
                     VLGGDVFRVLERVGGERERPFVCVNVDMVGGVAADASGVRFLSKHVDGIISTHRHVIE
                     LAKGAGLFTVHRLFAIDSSAVERGLKLIHRAEPASVEILPGLAYPEISASYRKALHQP
                     MLAGGLIKNQEAVTRILKAGAAGVSTSDPALWKHA"
     gene            3630..4514
                     /gene="phnD"
                     /locus_tag="VFJ72_05305"
     CDS             3630..4514
                     /gene="phnD"
                     /locus_tag="VFJ72_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7478917.1"
                     /translation="MKGKLCAIVAASLFVALLSGCGGSSSDASSSGEDALQVGLIPNQ
                     NPEKVEAQYKPLEDYLSKKLDRKVEVSVPTTYNAVVEAMTSGKLDLAYFGGLTYVQAR
                     QRAEIHPLVTEINPETHNTKYHSLIIAPKDGDVRKVEDIKGKTFAFGSVSSTSGSLYP
                     SIMLRDAGIDYRTDLKDKIYTGGHDATALAVANGRVDAGGVEDRILHGLEKDGVVDKS
                     KIRVIEQSAPIEGYPWVVRDALSDKTKKEITNAFLDIKDPKLLDLLRAEGYEKVKASD
                     YDYIEEQARKLDLLAEKQ"
     gene            4489..5364
                     /locus_tag="VFJ72_05310"
     CDS             4489..5364
                     /locus_tag="VFJ72_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7478918.1"
                     /translation="MTFSPKNSNVPSLSLEAVSVRYGGVAALEDVSLAVEPGEQLAVI
                     GASGAGKSTLFRALTRDAPLAAGRIFIGERDLYRLSSRELKKVRRRIGTIYQAYNLVP
                     QLSAGVNTSLGEVGGMRALETLRTFVAGPAPTLSKRVRTALEEVGLGDKTTVRTADLS
                     GGQQQRVAVARLLVQRPDLILADEPFAAVDPVTTERVLETLLRLNSDGATLLVNLHDV
                     EIARRFPRIAALREGRIVFDGPSDTLSEDMLKEIYAGDPGPGNAALPDSNGDLRRRRE
                     EQTRLLEGQDGVSAH"
     gene            5345..>6173
                     /gene="phnE"
                     /locus_tag="VFJ72_05315"
     CDS             5345..>6173
                     /gene="phnE"
                     /locus_tag="VFJ72_05315"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HET7478919.1"
                     /translation="MASPRTEQPGIGGAVPRFSWKSSLTVLVVLGLTGYSVYGTGFDL
                     VELLSNAGPVERFVSEMFPPDLSVATLAATGTGILETFQMSFLGALIGAVVAFPLAAL
                     GTQEISTTGTSRGERLLKSIPYHLSRFVLNVFRSVPDILWALVFVVALGLGPFPGTLA
                     LAVHSAGVLGKLYSETLEAVPSRPVEALRATGAGGFQSFLFGRLPQAMSGFTSLTLYQ
                     WECNIRSATILGFVGAGGIGQQILISMNLFDYPKVATLVGATIIVVLLVDRFSAAVRR
                     "
BASE COUNT         1022 a         2145 c         1865 g         1141 t
ORIGIN      
        1 cgacgtcgca cggcgcggtc aggtatccct tcatctcctc gtcgagcttg agcagcgtga
       61 tagagaagcc cgccatctcc agcgaggtca tgtaatcgcc gacgtagggc atcctgaaga
      121 cgttgacgcc ctcgttcttg agcacatcgg cgatgtggga gtaagcgacg tatagctcca
      181 tgatgggggt gccgcccatg ccgttcacca tcacggccac gtcggagccg gagaagtcca
      241 cgttgtcgcc caggatcttg ccgagcatct cgtccacgat gtcgttggcg ggctctatct
      301 tcttgcgttc ggtgccgggc tcgccgtgaa tgcccatccc gatctccatc tcgtcgtccc
      361 cgatctggaa gatgggctcc cccgcctcgg gcggcgtgca gctcgtgagc gccatcccca
      421 tcgagcggac gttggcgttg accttctcgg ccagttcctt gatgccggcc aggtctttcc
      481 catcgtcggc cgccgcgccg cagatcttgt gcacgaagat ggtccccgca accccgcgtc
      541 gcccactggt gtacgtagag tcctccacgg ccacgtcgtc gttcactacc acgtagtcag
      601 tctggattcc ctcggcctcg gccagctccc cggccatctc gaagttgagg acgtcgccgg
      661 tgtagttctt gacgacgtag aacacgcccg cgccgccgtc caccgccttg gttgcttcca
      721 gcatctggtc cggggtgggg ctcgtgaaga ctgcgcccgc gcaggcggcg tcgagcatac
      781 cggggccgac gtagccgccg tgggtcggct cgtggccgga gccgccgcca gagataagcg
      841 cgaccttccc ctgcaccggg gcgtcggcgc gcacgacgat gctgtgctcg gggatcacgc
      901 ggataagacc gccgtgcgcc agctccatcc cccgcagcat ctcttcgacg accttttcag
      961 gcgcgttgat gagcttcttc atctaattcc tttcttcccg gcgcagtccc gacgggagaa
     1021 cctcccgccg ggaccacact cgaacttccc taggtgagcg gcgactcgac ggcttcctgc
     1081 gcccagttct tggcccgctt gacggcttcc tgccaccgtc cgtacagccg ctcgcgctcc
     1141 tcctcgtcca tccgcggctc gtagcggcgc ctcagacgcc accggctctt cagctcctcc
     1201 ttgtccgacc agaaaccgac cgcgagcccg gcgaggtacg ccgcgccgag agccgtagtt
     1261 tcgagcgtct ccggcacctc gaccggcacg cccaggatgt cggcctggaa ctgcatcgtc
     1321 cagcggttgg cggcggctcc tccgtcggcc ttgaaggtcg ggatgtctat gccggagtca
     1381 agctccatcg cctcgaccgc atcgcgggtc tggtagcaca tgctctcaag cgccgcccgc
     1441 gccaagtgcg cccgcgtcgt gccgcgcgtg agcccgacta tggtgccccg cgcatacgag
     1501 tcccagtgcg gcgccccgag cccgacgagt ccggggacga agtacacgtc gtcgtttgag
     1561 tcgaggctct gggccagctc ctccgtctcg gaggcgtcct ttatgatgcc gagcccgtcc
     1621 ctgagccact gcacggcggc gccggtgatg aagatggcgc cttcgagggc gtactcgacg
     1681 ggttcgtcgc cgaggcccca ggcgatggtg gtgagcatct gacgctcgct ctttatgggc
     1741 tcggtgccgg tgttcatgag ggcgaaggag ccggtcccgt aggtgttctt ggtcaggccc
     1801 ttctcgaagg cgacctcgcc gaagagggcg gcctgctggt ctccggcaac gcccgcgacc
     1861 gggacctccg cgccgaagac ctcagcgtca gtgttgccat agacgaatga gctcggcttg
     1921 acctcgggga gcatctctcg cgggacgccg ccgagcatct ccagcagctc gtcgtcccac
     1981 ttgaggtcga agatgttgta catcagggtg cgcgaggcgt tcgagtagtc ggtcacgtgc
     2041 tcccggccgc cggtgagctt ccagatcaac cacgtgtcca tcgtgccgag ggccagctcg
     2101 ccgttctgcg cccgctcctt gagcccgtcg atgttatcga gcatccactt gatctttgac
     2161 ccggagaagt atgcgtctat gaccaggccg gtcttgttcg caaccatctc gtcgttaccg
     2221 gcctcggaga gttcggtgca gatctgggct ccgcggcggt cctgccatac gatggcgttg
     2281 tggaccggct cgccggtctc cctgtcccac acggccacgg tctcgcgctg gttggtgatg
     2341 ccaatggctg cgatctggtc cggcgacacc cccgcgtcat ctagcgcctt ccgcatcacc
     2401 ccgagggtga cctcccagat ctcgttggcg tcgtgctcta cccatccggg cttcgggtag
     2461 tgctgcgtga actcctgctg cgcctccccg accgtcgcgc tgtcatggtt gaacactatc
     2521 gccctgctcg acgtggtccc ctggtctata gccagtatgt attcccctgc catgctctat
     2581 cctttctctc ctttcccacc acgaaccgca accctcttta ttcgaccccg aggatcacct
     2641 ccttcaccac ctcacaagaa tagccccgcg cgagaccacg cgccagcata gtgcctgagc
     2701 cggtgatccc gcgcggggct tatctctgtt ttctctgcgc cgccgcaacg cctattgctt
     2761 tgtggtttcg agcttacgcc gaccgggaac ccgcgtcaag catgcttcca tagcgcgggg
     2821 tcgctggtcg agacgcccgc ggctccagcc ttcagtatcc gcgttaccgc ctcctggttc
     2881 ttgataagtc cgcccgccag catcggctga tggagcgcct tgcgatagct cgcggagatc
     2941 tcggggtagg cgaggcccgg aagtatctca acgctggccg gttcggcgcg gtgtatgagc
     3001 ttcagccccc gctctacggc gctggagtct atggcgaaga gccggtgcac cgtgaacagc
     3061 cccgccccct tcgccagctc gatcacgtgc cggtgcgtgg atatgatccc atcaacatgc
     3121 ttcgatagga accgcacacc actcgcatcc gccgccacac caccgaccat gtccacgtta
     3181 acgcagacaa acggccgctc ccgctctccg ccgacccgct ccagcacccg gaacacatca
     3241 ccaccgagaa cgaagatcgc cgccggttgt gtctccaggg cgtgatccag ggcaccgtcc
     3301 ggcccacgca cggccggtat caccggattc gcccgcagcg catcgaagaa cctatccgct
     3361 ttggatcttg ccgtattctc cacgaacgga ctctagcatc ctcgtgcgtc acgagtaact
     3421 tgtccggaga tccttgtcca gcgatccagc ctttctatct ttgcctggat gccggatctg
     3481 ttcagatcat tcgattgaac gtacaggtgc cgcgtgataa cttccgtgga cccagacagc
     3541 ggaggctctc cgcgccagcg taaaataacc cgcgcgttcc ggatggccgg gtgagaagac
     3601 catcggggtc gtgaatggaa ggatggaccg tgaagggaaa gctctgcgcc atcgtggcgg
     3661 cctctctatt tgtcgcgctg ctctcgggat gcggcggatc ttcgagcgac gcatcttcga
     3721 gcggtgaaga tgcgctccag gttggcctga tcccgaacca gaacccggaa aaggtcgagg
     3781 cgcagtacaa gccgctggaa gactacctct ccaagaaact ggatcgcaag gtcgaggtct
     3841 cggtcccgac cacctacaac gccgtcgtcg aggcgatgac ctccggtaag ctggacctgg
     3901 cgtacttcgg cggtctcacc tacgtgcagg cgcgccagcg ggcggagata cacccgctcg
     3961 tcaccgagat aaaccccgag acgcacaaca caaagtacca ctccctgatc atcgcgccaa
     4021 aggacggcga cgtcaggaaa gtcgaggaca taaagggcaa gaccttcgcc ttcggcagcg
     4081 tctcctctac ctcgggttcg ctttaccctt cgatcatgct gagagacgcc ggcatagact
     4141 acaggacgga cctgaaagac aagatctaca ccggcggcca cgatgccacg gccctggcgg
     4201 tggcgaatgg ccgggtggac gcggggggcg tggaggaccg catcctgcac ggtcttgaga
     4261 aggacggcgt ggtggacaag agcaagatcc gggtgataga gcagtccgcg cccatcgagg
     4321 gctacccgtg ggtggtgcgc gacgcgctct ccgacaagac gaagaaggag ataacgaacg
     4381 ccttcctgga cataaaggac ccgaagctcc tcgaccttct ccgggccgag ggatacgaga
     4441 aagtgaaggc ttcggactac gactacatcg aagagcaagc ccgcaaactt gaccttctcg
     4501 ccgaaaaaca gtaacgtccc gagcctctct ttagaggccg tctcggtccg ctacggcggc
     4561 gtggcggccc tggaagacgt gagcctcgcg gtggagccgg gagagcaact cgccgtcatc
     4621 ggagcgtcgg gagcgggcaa gtccaccctc ttccgcgccc tgacgcgcga cgcgccgctc
     4681 gccgcgggcc gcatcttcat cggcgaacgc gacctgtaca ggctgtcgag ccgtgagctg
     4741 aagaaggttc ggcgcaggat cggcactatc taccaggcgt acaacctcgt cccgcagctc
     4801 tccgccggcg tcaacacctc tctcggcgaa gtcggcggga tgcgagccct ggagacgctg
     4861 cggaccttcg tcgccggacc cgcccccacc ctctccaaga gggtccggac agcgctggag
     4921 gaggtgggtc tcggggacaa gaccaccgtc cgcacggcgg acctctcggg cgggcagcag
     4981 cagcgggtgg cggtcgcgcg gctgctggtg cagcgcccgg acctgatcct ggccgacgag
     5041 cccttcgccg ccgtggaccc tgtcaccacc gagcgcgtcc tggagacgct gctgcggctc
     5101 aacagcgacg gcgctacgct cctggtcaac ctacacgacg tggagatagc gcgccgcttc
     5161 ccgcgcatcg cggcgctgcg cgagggccgt atcgtcttcg acggcccgtc cgataccctg
     5221 agcgaggata tgctcaagga gatctacgcc ggagaccccg gcccagggaa cgcggcgctc
     5281 ccggactcga acggagacct acgccgccgc cgagaagaac agacccgctt actggaggga
     5341 caagatggcg tctccgcgca ctgagcaacc gggcatcgga ggcgcggtcc cgcgcttctc
     5401 ctggaagagt agcctcaccg tgctggtggt cctcggccta accggataca gcgtctacgg
     5461 cactggcttc gatctcgtcg agctgctctc caacgccgga cccgtcgaac ggttcgtctc
     5521 cgagatgttc ccgccggacc tctccgtggc taccctcgcg gcgaccggca cggggatact
     5581 ggagaccttc cagatgtcgt tcctcggggc gctcatcggc gccgtcgtcg cgttcccgct
     5641 cgcggcgctg gggacgcagg agatctccac taccggcacc tcccgcggcg agcgtctgct
     5701 taagagcatc ccctaccacc tctcgcgctt cgtcctcaac gtcttccgct ccgtaccgga
     5761 catcctctgg gcgctcgtct tcgtcgtcgc cctcggcctc ggcccgttcc ccggaaccct
     5821 cgccctcgcc gtccactcgg cgggcgtcct cggcaagctc tacagcgaga ccctcgaagc
     5881 cgtcccctcc cgccccgtcg aagcgttgcg cgccacgggg gccggcggct tccagtcctt
     5941 cctcttcggg cggctgccgc aggccatgag cggcttcacc tcgctcactc tctaccagtg
     6001 ggagtgcaac atccgctccg cgaccatact aggtttcgtg ggcgcgggcg gcatcgggca
     6061 gcagatcctc atctccatga acctcttcga ctacccgaaa gtagctacac tggtaggtgc
     6121 gaccatcata gtcgtgctgc tcgtggaccg tttctccgcg gcggtgcggc gac
//
LOCUS       DASSMB010000082        13436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_271513, whole genome shotgun sequence.
ACCESSION   DASSMB010000082 DASSMB010000000
VERSION     DASSMB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13436
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271513"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VFJ72_05320"
     CDS             <1..646
                     /locus_tag="VFJ72_05320"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HET7478920.1"
                     /translation="IKITGSQRLDILGVKKEDLPKAWEDLDMPSGFAYTKAFRQVKSC
                     VGTDFCRYGVGDSTKLAIDMEKEFERLYTPAKVKMGCSGCPRNCAEASVKDIGAIAIE
                     GGWQVYVGGAAGMEVRKGDVIATVETEKEAKDVMISFMQYYRENGHYKERTYDFVPRI
                     GLEKIQEVILNEESGEPERLRERLRQAKEATSDPWLERRSNATKNQFVGTIGRK"
     gene            643..999
                     /gene="nirD"
                     /locus_tag="VFJ72_05325"
     CDS             643..999
                     /gene="nirD"
                     /locus_tag="VFJ72_05325"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HET7478921.1"
                     /translation="MSRWIEVCATEDIPRLEGRRVVIECFRIGVFNTEEGFFAIGDVC
                     PHRGGPLSDGDVAATTVSCPLHNRKIELTSGEVKNDDLSPVLAFPVKVEGGKVLVDAE
                     ALYNRDDAVDEEEAVA"
     gene            996..2057
                     /locus_tag="VFJ72_05330"
     CDS             996..2057
                     /locus_tag="VFJ72_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478922.1"
                     /translation="MSAGEEFREILKQRARGPTHLGELDPALCERAMELILSRAATPA
                     QIGGFLLVGRAAGDSAAEIAAYTRPMRALARRIDAPSGPPVVTLTGGFDGKLRTFNVG
                     AAASLVAAAAGGRTLLLGGENTPPKAGRTVFDALRGLGFAAPQGLEEAANSLTEHGIA
                     ATSVEHYLPELHALLGLRWEMARRTVLSVCEKIVSPVPETRWMIGVSHRPFLESVSRA
                     LIELGVRHALVYQAIEGSDEAPLDGSSSLVRIRDGEVEEFSVEPESLGLIRATKAHIP
                     WRNEEDETRNLLAALGGAGAEPVRALILYNAALRLWMGAPDTSSPLDGHLERAEAALE
                     SGAAVDLLERAQRAVTLPG"
     gene            complement(2064..2204)
                     /locus_tag="VFJ72_05335"
     CDS             complement(2064..2204)
                     /locus_tag="VFJ72_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478923.1"
                     /translation="MNKADIAIKILKSPRARRTVVKALKNEHIRKIVIKQVKRRVLGT
                     KK"
     gene            complement(2222..3256)
                     /locus_tag="VFJ72_05340"
     CDS             complement(2222..3256)
                     /locus_tag="VFJ72_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478924.1"
                     /translation="MGEHVLFRTGIRRLGTKERGFFYRYPDSGETVREEKVLTRIENL
                     KVPPAWEDARIARGPSAKVQAVGYDSAGRLQYLYSERYRARKEHEKFERVLRFADALP
                     KMRKTTSDHLRRKELEREKVLAAMVRLINAAYFRVGEERYAKHNRTYGIATLRRKHLT
                     IEGDTMIFEYKGKWGQEHRKAITDARLRSVVEECVDLPGYEVFKYMDESGEVRDVKSR
                     DLNAYIKEVMGEEFTAKDFRTWAGTLIAATTLARLGPVGEPKSAQKNVLEAVDTVAHR
                     LGNTRDIARTSYISPKVIDHYMEGSVVAYYAEKLEEVIAAEQGDLTEEERALLELLNK
                     KLRRELKKAA"
     gene            complement(3349..4257)
                     /locus_tag="VFJ72_05345"
     CDS             complement(3349..4257)
                     /locus_tag="VFJ72_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478925.1"
                     /translation="MTDRPEDRSEARRRTGPLGDEEETRMDEREPGRAEQAEQESRAS
                     DDEATRQVPTESAERREDDAASAPEDESAAEDTGPLSRRERRQARKGEEEDKETRVIR
                     TPGSGTTRTQEGAAYPRGYFEAAEEREDRLRDMYGGVDWLASFLGFVFAVVAASFLSA
                     IAGLILIPLGLSLDLSSGSLGPAAITGLVITGILVFLMFFFGGYVAGRLARFDGGRNG
                     MMTVVWTIAAMVAVVAVSGFLPGGVFDSFRNFMETSVLPLLGDLSNLGIAGIVIIVAI
                     ILIAVLGGLFGGRVGSRYHSEIDRTT"
     gene            4609..5940
                     /locus_tag="VFJ72_05350"
     CDS             4609..5940
                     /locus_tag="VFJ72_05350"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET7478926.1"
                     /translation="MASLGRDDVIKQAQESNVQFIRLWFTDVLGVLKSFAITAEELED
                     ALDGGMGFDGSSITGYQDIEESDMIAMPDPSTFQIIPWSPQDEPTARMICDVRTPDGD
                     PYVGDPRHILRRALERASDMGFDNYYCGPELEFFYFKDSKGTEPLDYGGYFDLTTLDA
                     ATALRRETVQSLQQLGIKVEYSHHEVGISQHEIDLRFDDAMTMADKVMTYKTVVKEIA
                     TRHGVYATFMPKPLMDQNGSGMHTHQSLFTGGKNAFFDADAEYYLSDEAKAFIAGQLR
                     HARELSIIFAQHVNSYKRLVPGYEAPVYIAWSRRNRSALVRVPLYHPGQEKATRMELR
                     CPDPSANIYLCFAALLHAGLEGIEKGYELPEPMERNLYHLTSEEREKMGIQSLPADLG
                     EAIKEAEHSELLQKALGEHTYTRLLELKREEFEDYRIQVTPYELEKFLPVL"
     gene            6108..7589
                     /locus_tag="VFJ72_05355"
     CDS             6108..7589
                     /locus_tag="VFJ72_05355"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET7478927.1"
                     /translation="MVRIATRRSALEDVTADLMAIGLFENEEPPAPLMEVAGVAVSSG
                     DFSGKRGETTLLYAGDSISSPRLLLLGLGDRGAFTAERLRRAAATVARRARSAKLESA
                     AFALPLLEQIDSRTAAQAAAEGAALGLYRFTRYKTGAGNADVRELETFDLILHDGADE
                     SEAARGAEIGAAMAAGANLARDLANEPSNTATPEYLAERAREIAARHGMQVEVLDRAG
                     IEDVELVGLATVGRSAANEPRFIVLEHRGGGDEAPVVLVGKAVTFDSGGISIKPSAGM
                     EDMKFDMSGGAAVLGAMETAGTLGLPINVVALVPATENLPGGSAFKPGDVLRMRSGKT
                     VEILSTDAEGRLILADALAYARRYEPAAVIDCATLTGACIVALGHHASGLMGNDEALI
                     SEVQAAGERAGERAWPLPLFEEYTKQIKGDTADIKNTGGRYGGALTAGAFLKEFADYP
                     WAHLDVAGTAFGKKGNSYTPKGATGAPARLLVEFLNSRIGGDA"
     gene            7586..8224
                     /locus_tag="VFJ72_05360"
     CDS             7586..8224
                     /locus_tag="VFJ72_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7478928.1"
                     /translation="MILEMLTVGPFQENCYIVGDDGTGTGVLVDPGDEAARISLAVEQ
                     TQLEIEKIVITHAHIDHVGAVAALVDEYACPVLMHAEAEPMLKQLPTQAMMMGLRFGK
                     VPTVDGYIEDEEVVDVGGLRLRALYTPGHAPGHLAFYEENEGFVLSGDALFAGSVGRV
                     DLPGGSMEVLMRSIEERLMTLPDETLVYSGHGPRTTIGEERAHNPFLRGGLL"
     gene            8221..8745
                     /locus_tag="VFJ72_05365"
     CDS             8221..8745
                     /locus_tag="VFJ72_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET7478929.1"
                     /translation="MSESVHKHRESAPETVRVAVLTISDTRTQENDTGGDTIEELMRA
                     AGHEVVERTIVRDDASAIRTVLVNLLANSEVDAVITTGGTGISARDTTYEVVDRMVEK
                     RLDGFGEIFRMLSYEEIGAAAILSRAIAGAVGTKLVASLPGSRNGVRLAVQKLLVPEI
                     AHIVFELRKHQESR"
     gene            8751..9011
                     /locus_tag="VFJ72_05370"
     CDS             8751..9011
                     /locus_tag="VFJ72_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478930.1"
                     /translation="MEREPLSPEAEALWRNLWEIWQDNDEDDVVLDSSKIGDLEEKIP
                     ALEGRMKTALAYLQRARYIQYRSGVGEDGIEPILYDVYEPRN"
     gene            9024..9470
                     /locus_tag="VFJ72_05375"
     CDS             9024..9470
                     /locus_tag="VFJ72_05375"
                     /inference="COORDINATES: protein motif:HMM:NF016535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7478931.1"
                     /translation="MGWVLLIPLALAAGMALSTQFAVNTQLKNVVGGPVAAAAVSFLI
                     GTVFLTVAALAVTRSMPTLGEITASPWWIWIGGLLGAFFVFASVVLTPRLGAATTVGL
                     FLTGQVTASIILDHFGLLRIPVHEATLPRLAGAALIIVGVVLVQRF"
     gene            9475..10056
                     /locus_tag="VFJ72_05380"
     CDS             9475..10056
                     /locus_tag="VFJ72_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7478932.1"
                     /translation="MRSICVFCGSRPGADPAYAEAAGALGRALAEAGVTLVYGGGHVG
                     LMGVVADAALGAGGEVIGVMTRALVEREIAHTGLTKLHVVVSMHERKALMADLSEGFI
                     ALPGGTGTLEEFFEVLTWAQLGEHQKPCGLLNIAGYYDPLLTVFDHMVERGFVSREHR
                     RLVLVESGPTAMLDAFARYSPPKTVKWLTPEET"
     gene            complement(10107..11135)
                     /gene="fba"
                     /locus_tag="VFJ72_05385"
     CDS             complement(10107..11135)
                     /gene="fba"
                     /locus_tag="VFJ72_05385"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01521.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET7478933.1"
                     /translation="MALVTMRQLLDEAAKGNYGVGAFNVNNLEQIQAIMEAAQETNSP
                     VIIQASRGARKYANDRFLYHLMLAAAEVYPDIPVALHQDHGNGPATCKSAIDLGFTSV
                     MMDGSLMEDGKTPADFEYNVRVTREVVEMAHERGVTVEGELGTLGGIEDGVGSGEVHL
                     TDPDQAVEFVERTGVDALAVAIGTSHGAYKFTTEPDRDVLAMHIIEEINQRLPNTHLV
                     MHGSSSVPKELVDIINQYGGEIRPTFGVPVREIQEGIKHGVRKVNVDTDLRLASTGAI
                     RKAFAEDPGEFDPRYYLKPAREAMQEVVKARMEAFGQAGHASDYEPLTLEEMAERYQK
                     EGHELTAT"
     gene            complement(11283..12041)
                     /locus_tag="VFJ72_05390"
     CDS             complement(11283..12041)
                     /locus_tag="VFJ72_05390"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET7478934.1"
                     /translation="MSKIARVKSPDIAQAPEAIAKPKPPKPAVVKVPDIPYNATEKEA
                     KKMLEDNGLKLGKVETKHNDEYDKGGVFYQDPLPGIQVKEGDSVGITLSSGPEKKKDD
                     GDGGVPDPPTDDLYLTVPKMGLTDNYVTNTDDEAAMDSGAIKLTSTGFPWQPHANTYI
                     AAHVLGYSGTGSYMQFAGLPSMTYGDEIDLKDANGTVYKYRVSEILTVTPEENWVTAP
                     VPNKDMVTLQTCVNPPYYDQRLIVRAERVEVDKA"
     gene            12429..13406
                     /locus_tag="VFJ72_05395"
     CDS             12429..13406
                     /locus_tag="VFJ72_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7478935.1"
                     /translation="MGLLDTGTEKETRGRAAETTLLARGLKKSYGDFEALKGVDFEVY
                     RGECFGFLGPNGAGKTTTMKTIYGAAIPTSGELSVVGLDIRRDERAVKRRIGVIPQEN
                     NLDEDLKVRENMLVYGRYFDLPKKVARQRADELLDFVQLTEKAGAEVGELSGGMKRRL
                     LIARALINDPDLVVLDEPTTGLDPQARHLVWDKLRELALRGKTLILTTHYMEEAARLC
                     DRLVIMEGGRIIAGGTPASLISEHVSPQVLEFRSDTESLQKLRPVLDREADNVEESGS
                     GEALLAFTSDADSLMETVRRSGIQVENTVYRQSGLEDVFLRLTGRSLTD"
BASE COUNT         2403 a         4287 c         4357 g         2389 t
ORIGIN      
        1 gatcaagata accggctccc agcgcctcga tatcctgggc gtgaagaaag aagacctgcc
       61 gaaagcctgg gaggacctgg acatgccctc cgggttcgcg tacacgaagg cgttccggca
      121 ggtcaagagc tgcgtgggga cggacttctg ccgctacggg gtcggcgact cgacgaagct
      181 ggcgatagac atggagaagg agttcgagcg gctctacacg ccggcgaagg tgaagatggg
      241 ctgctcggga tgtccgcgca actgcgccga ggcgagcgtc aaggacatcg gcgctatcgc
      301 catcgagggc ggatggcagg tatatgtagg gggcgcggcc gggatggagg ttcggaaggg
      361 agatgtgatc gccacggtcg agaccgagaa ggaggcgaag gacgtcatga tctctttcat
      421 gcagtactac cgggagaacg gacactacaa ggagcgcacc tacgacttcg tacccaggat
      481 cggtcttgag aagatccagg aggtcatcct gaacgaggag tccggagagc cggagcgcct
      541 gcgagaacgt ctgcgtcagg cgaaggaggc caccagcgac ccgtggctgg agcgaaggag
      601 caacgccaca aagaaccagt tcgtcggaac gataggccgt aaatgagccg ctggatcgag
      661 gtctgcgcca cggaggacat tccccgcttg gaaggccggc gggtcgtgat cgagtgcttc
      721 cgcatcgggg tcttcaacac cgaggaagga ttcttcgcca tcggggacgt ctgcccgcac
      781 cggggcggcc cgctctcgga cggcgacgtg gccgccacga ccgtctcctg cccgctccac
      841 aaccgaaaga tcgagctgac gtccggcgag gtaaagaacg acgacctctc ccccgtactc
      901 gcgttcccgg tgaaggtcga gggcggcaag gttctcgttg acgcggaggc gctgtacaat
      961 cgggacgatg ccgtagacga ggaggaagcc gtggcgtgag cgcgggcgag gagttccgcg
     1021 aaatattgaa gcagcgggcg cggggaccga cgcacctggg agagctggac ccggcgctct
     1081 gcgagcgggc catggagcta atactctcca gggcggccac ccccgcccag atcgggggtt
     1141 tcctgctcgt cggccgcgcc gccggcgact ccgccgccga gatagccgcc tacacccgtc
     1201 cgatgcgtgc gctcgccagg aggatagacg ctccctccgg cccgccggta gtcaccctca
     1261 ccggcggctt cgacgggaag ctccgcacct tcaacgtcgg agcggcggct tcactagttg
     1321 cagcggcagc cggcggacgg acactgctac tcgggggcga gaacacgccg ccgaaggccg
     1381 gacgcacggt cttcgacgcc ctgcgcgggc tcggattcgc cgctccgcag ggtctcgaag
     1441 aagcggcaaa ctcgctcacc gagcacggga tcgcggcgac cagcgtggag cactacctgc
     1501 cggagctgca cgcgctgctg ggattgcgat gggagatggc ccgccgcacg gttttgagcg
     1561 tctgcgagaa gatcgtctcg cccgtgccgg agacgcggtg gatgatcggc gtctcgcacc
     1621 ggccgttcct ggagtcggtc tctcgggcgc taatcgagct gggcgtgcgg cacgcgctcg
     1681 tctaccaggc gatcgagggc tccgacgaag cgccgctcga tggtagctcg tcgctggtgc
     1741 gaatccggga cggcgaggtc gaggagttca gcgtggagcc ggagtcactg ggactcatac
     1801 gggccacgaa ggcgcacata ccctggcgga atgaggagga cgagacccgg aacctgctcg
     1861 cggcgctcgg gggcgccggg gcggagccgg tccgggccct gatcctgtac aacgccgcgc
     1921 tgcggctgtg gatgggcgct ccagacacgt catcgccgct cgatggccac ctggagcgcg
     1981 cggaagccgc gcttgagtct ggcgcggctg tggatctctt agagcgggcg cagagagcgg
     2041 taactcttcc cggatagggt ctgctacttc ttcgtgccga gaacgcggcg cttgacctgc
     2101 ttgatcacta tcttgcggat gtgctcgttc ttgagggcct tgacgaccgt ccggcgagcg
     2161 cgcgggctct tgaggatctt tatcgctatg tccgccttgt tcatcctgcc tcctgtctct
     2221 gttaagcggc ctttttcaac tcgcggcgga gcttcttgtt cagcaactcc agaagggcgc
     2281 gctcttcttc ggtgaggtcg ccctgctcgg ccgcgatgac ctcttccaac ttctctgcgt
     2341 agtaggctac cacggagcct tccatgtagt ggtctatcac cttcgggctg atgtacgaag
     2401 tgcgggcaat gtcccgcgtg ttaccgagcc tgtgcgccac ggtatcgacc gcctccagca
     2461 cgttcttctg ggcgctcttt ggctcgccga ccggtccgag ccgagccagc gtggtcgcag
     2521 cgatcagagt ccccgcccag gtacggaaat ccttcgcggt gaactcctcc cccatgacct
     2581 cctttatgta ggcattgagg tcgcgggact tcacgtccct cacttcgccg gactcgtcca
     2641 tgtacttgaa gacctcgtat ccgggcagat ctacgcattc ctcgacgacg ctccgaagcc
     2701 gggcgtccgt gatggccttg cgatgctcct ggccccactt gcccttgtac tcgaagatca
     2761 tggtgtcgcc ctctatggtg agatgcttgc ggcgcagggt ggctattccg taggtccggt
     2821 tgtgcttcgc gtagcgctcc tcgccaaccc ggaagtatgc cgcgttgatg aggcgcacca
     2881 tcgcggcgag gaccttctcc cgctcaagct ccttgcgccg caggtggtcg ctggtggtct
     2941 tgcgcatctt cggcagggcg tcggcgaagc gcagaacccg ctcgaacttc tcgtgctcct
     3001 tgcgcgcccg gtagcgttca ctgtacaggt actgcagccg ccccgccgag tcatagccga
     3061 ccgcctgcac cttcgcgctc ggcccgcgcg ctatgcgagc gtcctcccag gccggcggca
     3121 ccttcaagtt ctctatccgc gtgaggactt tctcctcgcg caccgtctcg ccggagtccg
     3181 ggtaacggta gaagaacccc cgctccttcg tgcccagacg ccggatgccg gtcctgaata
     3241 gaacgtgctc gcccactcac cacctctcga atctctatag aaccgcatcg gtttataccc
     3301 gattagccag cggattgcgt acgctctagc tctggcggcg cgctccggct aggtcgtgcg
     3361 gtcaatctcc gagtgatagc ggctcccgac ccgcccgccg aagagaccgc ccagaaccgc
     3421 gatcagtatt atcgcgacga tgatcacgat cccggcgata cccaggttgg agaggtcccc
     3481 gagcagtgga agaaccgagg tctccataaa gttgcggaag ctgtcgaaga cgccacccgg
     3541 caagaaaccg ctgactgcaa cgaccgccac catagcggct atcgtccaca cgaccgtcat
     3601 catgccgttg cggccgccgt cgaagcgcgc cagcctgccg gccacgtaac ccccgaagaa
     3661 gaacatcagg aagacgagga taccggttat aacaaggccc gtgatcgccg ccggtccgag
     3721 cgagccggaa gagaggtcca ggctgaggcc cagcgggatc agtatgaggc ccgcgatggc
     3781 cgagaggaac gaagccgcca cgaccgcgaa gacgaacccg aggaagctcg cgagccagtc
     3841 cacgccgcca tacatgtcgc gcagccggtc ttcccgctcc tcggcggcct cgaagtaccc
     3901 ccgcggatac gccgcaccct cctgcgtcct cgtagtaccg gagcccggcg tgcggatcac
     3961 acgcgtctcc ttgtcctcct cttctccctt tctcgcctgc cggcgctccc gtctggagag
     4021 gggccccgtg tcttccgccg cggattcatc ctccggagcg gacgcggcat cgtcctcccg
     4081 gcgctccgcg ctctccgtgg gcacctgtcg cgtcgcctca tcgtccgacg cccgcgactc
     4141 ctgctcggcc tgttctgccc gaccaggctc gcgctcgtcc atccgggtct cttcctcgtc
     4201 tcccagcggc ccggtcctgc gccgcgcctc ggatctatcc tccggcctat ccgtcatctc
     4261 tccccctcgt ctccgcgcct gccggcactc cgaagtacaa cactccagtc aacacgctcg
     4321 ataacatgcc cgatatctcg ccactcaatt ctaccccgac agagcactta gctcattgat
     4381 atatcgtctg ttcccctttt ggggtactct tacgctacac atcgttagat gtcttgtctt
     4441 cccatgccaa ctattatacg ttgggccaaa aattcgcttc ttgcgcgaag cctggcttgt
     4501 acgccgggcc atatcaaagg tgggatcgcg ctcctatcat ggccggtgtt gttgtaagag
     4561 cgcccgattc gcgggacgat ttcggagcag gcgagcgtag gaggaatatt ggctagttta
     4621 ggtcgagacg acgtaatcaa gcaggctcag gagagcaacg tccagttcat ccggctgtgg
     4681 ttcacggacg tgctcggggt tctaaagagc ttcgcaatta cggccgagga gctagaggac
     4741 gccctggacg gcggtatggg cttcgacggt tcgtccatca ccgggtatca ggacatagaa
     4801 gagtctgaca tgatcgccat gcccgaccct tcgacgttcc agatcatccc ctggagcccg
     4861 caggacgagc cgacggcgcg tatgatctgc gacgtccgga ctcccgacgg cgacccttac
     4921 gtcggcgacc cgcgccacat cttgcggcgc gcgctggagc gggcgagcga catgggcttc
     4981 gacaactact actgcggacc ggagctcgag ttcttctact tcaaggactc caagggcacc
     5041 gagcccctcg attacggtgg ctatttcgac cttacgaccc tcgacgccgc cacggccttg
     5101 aggcgggaga cggtccagtc gctgcagcag ctcggcatca aggtcgagta ctcgcaccac
     5161 gaggtcggca tctcgcagca cgagatcgac ctgcgcttcg acgacgcgat gacgatggcc
     5221 gacaaggtga tgacctacaa gacggtcgtc aaggagatcg ccacccgtca cggcgtctac
     5281 gcgacgttca tgcccaagcc tctgatggac cagaacggct ctggcatgca cacccaccag
     5341 agcctcttca ccggcggcaa gaacgccttc ttcgacgcgg atgcggagta ctacctctcc
     5401 gacgaggcga aggcgttcat cgcgggtcag ctccggcacg cgcgagagct cagcataatc
     5461 ttcgcccagc acgtgaactc ctataagcgg ctggtccccg gctatgaggc cccggtctac
     5521 atcgcctgga gccgccgcaa ccgctcggcg ctggtccgcg tcccgctcta ccatccggga
     5581 caggagaagg ccacgcgcat ggagctgcgc tgcccggacc cgtcggcgaa catctacttg
     5641 tgcttcgcgg cgctcctgca cgcgggcctg gaaggcatcg agaagggcta cgagctcccc
     5701 gagccgatgg agcgcaacct gtaccatctc acgtccgagg agcgggagaa gatggggatc
     5761 cagagcctcc cggcagacct gggagaggcg atcaaggaag ccgagcactc cgagcttctt
     5821 cagaaggcgc tcggcgagca cacctacacc cggctcctgg aactgaagcg cgaggagttc
     5881 gaggactacc gcatccaggt aacgccctac gagctggaga agttcctgcc ggtcctctaa
     5941 ctagcagggg tattacgaag tatagggcgc ggagagatcc gcgcccttct ctatgccacc
     6001 ccgtcgctgt acagccgcac gttactggca gggctcggcg cgcctttccc gtataattcg
     6061 ggccgttcac gtcgaggcgt atcccatcgt tagagaggag atccggcttg gtccgtatcg
     6121 ctacacgaag atcggcgctc gaagatgtaa ccgccgacct catggcaatc ggcctgttcg
     6181 agaacgaaga gcctcccgca ccgctaatgg aggtggcggg cgtcgccgtc tcctcgggcg
     6241 acttcagcgg caaacgcgga gagacaaccc tgctgtatgc gggagactcg atctcctctc
     6301 cacgcctact gctactcggg ctcggcgacc ggggcgcctt cactgcggaa cgtctgcgcc
     6361 gggccgccgc taccgtcgcc cgccgggctc gctccgcgaa gctggagagc gcggccttcg
     6421 ccctgccttt gctggaacag atcgactcgc gtaccgcagc acaggccgcc gcggagggcg
     6481 cggcgctcgg cctgtaccgc ttcacgcgct acaagactgg cgccggaaac gcggacgtgc
     6541 gagagctcga aaccttcgac ctgatcctcc acgacggagc agatgagagc gaggcggctc
     6601 gcggcgcgga gatcggagcg gcgatggcag ccggagcgaa cctggcccgc gacctggcga
     6661 atgaaccatc caacacagca acaccggagt acctggccga acgggcgcgc gagatcgccg
     6721 cgaggcacgg catgcaggtg gaagtcctcg accgcgcagg cattgaggac gtggagctgg
     6781 tcgggctcgc gactgtaggg aggtccgcgg cgaacgaacc gcgatttatc gtgctggagc
     6841 accgcggggg cggagatgag gcgcccgtgg tgctcgtggg gaaggcggtc acgttcgact
     6901 ccggcggaat ctccataaag ccctccgcgg gtatggaaga catgaagttc gatatgagcg
     6961 gtggcgcggc ggtacttgga gcgatggaga cggcggggac gctgggcctt cccatcaacg
     7021 tcgtggcgct ggttccggcc acggagaacc tgcccggagg cagcgccttc aagccgggcg
     7081 atgtgttgcg gatgcggtcc gggaagaccg tcgagatcct ctccaccgac gccgaggggc
     7141 ggctcatcct ggcagacgcc ctcgcctacg cccggcgcta cgagccggcg gcagtcatag
     7201 attgcgcgac gctcaccggc gcgtgcatcg tggcgctcgg acaccacgcc tccggcctca
     7261 tgggcaacga cgaggccctg atctccgagg tccaggccgc cggggagagg gccggtgaac
     7321 gagcctggcc tctcccgctc ttcgaggagt acacgaagca gataaagggc gacacggcgg
     7381 acataaagaa caccggcggc cggtacggag gcgcactgac agccggagcg ttcctcaagg
     7441 agttcgcgga ctacccgtgg gcgcacctgg acgtcgcggg tacggcgttc ggcaagaaag
     7501 gcaactccta cacgccgaag ggcgcgacag gagcgccggc gcggttgctc gtcgagtttc
     7561 ttaacagccg cattggaggt gatgcatgat cctggagatg ctgacggtcg gaccgtttca
     7621 ggagaactgc tacatcgtcg gggacgatgg tacggggacc ggagtgctcg tggaccccgg
     7681 cgacgaggcc gcgaggatct cgctcgcggt cgagcagacg cagctggaga tagagaagat
     7741 cgtcataacc catgcccaca tagatcacgt cggagcagtg gccgcgctcg tggacgaata
     7801 cgcctgcccg gtcctgatgc acgccgaggc cgagccgatg ctgaagcaac tcccgaccca
     7861 ggcgatgatg atgggcctgc gcttcgggaa agtcccgacc gtggacggct acatcgagga
     7921 cgaggaggtc gtcgatgtcg ggggattgag gctgcgcgca ctctacacgc cgggacacgc
     7981 tccggggcac ctcgcattct acgaggagaa cgaggggttc gtgctgtcgg gagacgcgct
     8041 ctttgctggg agtgtcgggc gggtggacct ccccggagga agcatggagg tcctgatgag
     8101 gagcatcgag gagcggctta tgaccctgcc ggatgagacg ctcgtctact ccggccacgg
     8161 gccgcggacg acgatcggag aggagcgggc tcacaacccg ttcctgagag gagggctgct
     8221 gtgagcgaga gcgtccacaa gcaccgggag tcagcgccgg agaccgtccg ggtcgcggtg
     8281 ttgaccataa gcgatacgcg cacgcaggag aacgacacgg gcggagacac catcgaggaa
     8341 ttgatgcggg cggcgggcca cgaggtcgtg gagcggacca tcgtgaggga cgatgcgtcg
     8401 gcgatacgga ccgtgctcgt taacctgctc gcaaactccg aggtggacgc ggtgatcacg
     8461 accggcggga ccggcatctc ggcgcgcgac acgacgtatg aggtagtgga ccggatggtc
     8521 gagaagcggc tggacgggtt cggggagatc ttccgcatgc tctcctacga ggagatcgga
     8581 gcggcggcga tcctgagccg cgccatcgcc ggggccgtgg gaacgaagct ggtggcgagt
     8641 ctgccgggct cgcgcaacgg cgttcggctc gcggtgcaga agctgctcgt ccccgagata
     8701 gcgcacatcg tattcgagct acgcaagcac caggaaagca ggtaggacgc atggaacgcg
     8761 aaccgctctc acccgaggct gaggcgctgt ggcgcaacct gtgggagatc tggcaggaca
     8821 acgacgaaga cgacgtggtc ctggactcct cgaagatcgg ggacctggag gagaagatac
     8881 cggcgctcga aggacggatg aagacggcgc tcgcgtacct tcagcgcgcc cgctacatcc
     8941 agtaccgctc cggagtcgga gaggacggca tagagcctat actctacgac gtctacgaac
     9001 ccaggaatta ggaggtgaag aagatgggct gggtcctgct gatcccgctc gcgctcgcgg
     9061 ccgggatggc gctctccacg cagttcgccg tcaacacgca gctcaagaac gtcgtcggcg
     9121 gtccggtcgc cgccgcggct gtctcgttcc tgatcgggac ggtattcctg acggtcgcgg
     9181 cgctcgccgt gacgcgctcg atgccgaccc tcggcgagat cacggcctcc ccgtggtgga
     9241 tctggatcgg cggcctcctc ggcgccttct tcgtgttcgc ctcggtggtc ctcacacccc
     9301 gtctcggagc cgccaccacc gtcgggctct tccttaccgg tcaggttacc gcctccatca
     9361 tcctggacca cttcgggctg ctgaggattc ccgtccacga ggctacccta ccccgcctcg
     9421 cgggcgcagc cctcataatc gtcggcgtcg tgctcgtcca gaggttctag ccgggtgagg
     9481 agcatctgcg tcttctgcgg ctcccgtccc ggcgccgacc cggcctacgc agaagccgcc
     9541 ggagcgctcg gccgcgcgct cgcggaggct ggcgtcacgc tcgtctacgg cggcgggcac
     9601 gtcgggctta tgggggtggt ggcggatgct gcgctcggag ctggcggcga ggtgatcggg
     9661 gtgatgacga gggcgctggt ggagcgcgag atagcccata ccggccttac gaagctgcac
     9721 gtcgtcgtct ccatgcacga gcgaaaggct ctcatggcgg acctatccga aggtttcatc
     9781 gccctgcccg gcggcaccgg caccctggaa gagttcttcg aggtcctcac ctgggcgcag
     9841 ctcggcgaac accagaaacc ctgcggcctc ctcaacatcg ccggctacta cgacccgctg
     9901 ctgaccgtct tcgaccacat ggtagagaga ggcttcgtca gccgcgaaca ccgcaggctc
     9961 gtactcgtgg agtccggccc gaccgcgatg ctcgacgcct tcgcgcgcta cagcccgccg
    10021 aagaccgtaa agtggttaac cccggaggag acatagaaag cggccggctt ctggagccgg
    10081 ccgctgaaaa cgagacgctg aacgtatcag gtcgccgtga gctcgtggcc ttccttctga
    10141 tagcgctcgg ccatctcttc gagggtgagc ggctcatagt cgctggcgtg accggcctgg
    10201 ccgaaggctt ccatcctcgc ctttacgacc tcctgcatgg cctcgcgagc gggcttgagg
    10261 tagtaacgtg ggtcgaactc gccggggtct tcggcgaacg ccttgcggat ggcgcccgtg
    10321 gaggcgaggc gcaggtcggt gtccacgttg accttgcgga cgccgtgctt gatcccctcc
    10381 tggatctcgc gcaccggcac cccgaaggta ggacggatct cgcccccgta ctggttgatg
    10441 atgtccacta gctccttcgg cacgcttgag gagccgtgca tcacgaggtg cgtgttggga
    10501 agcctctggt tgatctcctc gatgatgtgc atcgcgagca cgtcgcgatc gggctccgtg
    10561 gtgaacttgt aagctccgtg gctcgtcccg atggcgaccg cgagcgcgtc aacgcctgtc
    10621 cgctccacga actccacggc ctggtccggg tccgtgaggt gtacctcgcc cgaaccgacg
    10681 ccgtcctcga tgccgccgag cgtcccgagc tctccctcga ccgtcacgcc gcgctcgtgg
    10741 gccatctcga cgacctcgcg ggtgacgcgc acgttgtact cgaagtcagc cggggtcttg
    10801 ccgtcctcca tcagggagcc gtccatcatc acgctcgtga agccgaggtc tatggcgctc
    10861 ttgcaggtcg ccggaccatt gccgtggtcc tggtgcagtg cgaccggaat gtccgggtag
    10921 acctccgccg ccgccagcat caggtggtag agaaagcggt cgttcgcgta cttccgggcg
    10981 cccctggaag cctggatgat caccggtgag ttcgtctcct gcgcggcctc catgatggcc
    11041 tgaatttgct ccaggttgtt gacgttgaag gcgcctactc cgtagttccc cttcgccgcc
    11101 tcgtcgagaa gctgccgcat cgtcacgaga gccataagat ctcctccgct cgcttgtgtt
    11161 cttcgcccgg aaacccctgc gtccggactg gaatgtgtag tatagacgct tccctaccct
    11221 acacacaaaa caagaaaaag gcgctcccga gagagcgccg gaccaatgcc ttttcttgag
    11281 ccctacgcct tgtctacctc gacgcgctcg gcgcgcacga tgagccgctg atcatagtag
    11341 ggcgggttga cgcaggtctg caacgtgacc atgtccttgt tggggacggg agccgtcacc
    11401 cagttttcct cgggcgttac cgtcaggatc tcgctgacgc ggtacttgta gacggtgccg
    11461 ttggcgtcct tcaggtcgat ctcatctccg taagtcatgc tcggtagccc cgcgaactgc
    11521 atgtatgagc cggtccccga atacccgagt acatgagccg ctatgtaggt gttcgcgtgc
    11581 ggctgccacg ggaacccggt gctcgtcagc ttgatcgccc cgctgtccat cgccgcctca
    11641 tcgtcggtgt tggtcacgta gttgtcggtg aggcccatct tcgggaccgt gagatacaga
    11701 tcatccgtgg gcggatccgg taccccgccg tcaccatcgt ctttcttttt ctctggcccg
    11761 gaacttagag tgattcccac agagtcgcct tcttttacct gaatccccgg caacgggtcc
    11821 tgatagaaga cgccaccctt gtcgtactcg tcgttatgct tcgtctcgac cttgccaagc
    11881 ttgaggccgt tgtcctcaag catcttcttt gcttccttct cggtcgcgtt gtacgggatg
    11941 tccgggacct ttacgaccgc gggcttgggc ggcttcggct tggcgatagc ctccggagcc
    12001 tgcgcgatgt cgggactctt caccctggct atcttgctca cctcgaccga tctctgggtg
    12061 ggatccaccc cgaacaacat caagccgccg agaacgacga taacaagcga tgcgaagaga
    12121 attagacctg tctgccgacg gcgcctgtac ttgcgcctct gcgatttgtt tcccatgcgc
    12181 gagattctag cagcgatgcc gggtacgcgg tagaggtccg cgcgtacatt tcttaaggat
    12241 tgtgaaaagt cctcttaagg tcgtaacatg agctacagaa acatgcatca tttggtcctt
    12301 gtctgttgat tctgcggcgg tcaagggggc cctcgggaga tgcgcggccg tgcggtagta
    12361 taagtgtctt cgcggccttg agtgtggcct ggatcgcatc gcaagtacat ttggcaaagg
    12421 aacggagctt gggcctcttg gacaccggta cggagaagga gactcgcggg cgcgcagcgg
    12481 agaccacgct gctagcgcgc ggactgaaga agagctacgg ggacttcgag gccctaaagg
    12541 gcgtggactt cgaggtgtac cggggcgagt gcttcgggtt cctgggcccg aacggcgcgg
    12601 gcaagacgac gacgatgaag actatctacg gagcggcgat ccccacgagc ggagagctgt
    12661 cggtggtcgg actggacatc cggcgcgatg agcgggccgt caagcggcgt atcggggtca
    12721 taccgcagga gaacaacctc gacgaagacc tcaaggtgcg cgagaacatg ctcgtctacg
    12781 gacggtactt cgacctgccc aagaaggtcg cccggcagcg ggccgatgag cttctggact
    12841 tcgtgcagct cacggagaag gccggggcgg aggtcgggga actctccggc ggcatgaagc
    12901 ggcggctcct catcgcccgc gccctcataa acgacccgga ccttgtcgtg ctcgacgagc
    12961 caaccacggg cctcgacccg caggcccggc atctggtgtg ggacaagctg cgcgagctgg
    13021 cgctgcgggg gaagaccctc atcctcacaa cgcactacat ggaggaggcg gcgaggctct
    13081 gcgaccggtt ggtgatcatg gagggcggcc gtataatcgc ggggggaact cctgccagcc
    13141 tgatctcgga gcatgtcagc ccgcaggtgc tggagttccg ctcggacaca gagtcgctcc
    13201 agaaactgcg tcccgtgctc gaccgcgagg cggataatgt ggaggagagc ggcagcgggg
    13261 aggcgctgct cgccttcacc tccgacgccg atagtctaat ggagacggtg cgccgctccg
    13321 gcattcaggt cgagaacacc gtctaccggc aatccgggct cgaagacgtg ttcctgcggc
    13381 tcactggaag gagcctgacc gactgatgaa tagcgcgatc aagattccgt cgcccc
//
LOCUS       DASSMB010000083         4442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_650687, whole genome shotgun sequence.
ACCESSION   DASSMB010000083 DASSMB010000000
VERSION     DASSMB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650687"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFJ72_05400"
     CDS             <1..339
                     /locus_tag="VFJ72_05400"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7478936.1"
                     /translation="GDMLGAASLGLHVLQMSGQSEIDEMLNLVTTRAARTLGIEDRYG
                     IEEGKPASLVIFDAKTPFDTLRLNPARLHVIKDGRTVAATEPARTTLRREGGRSETVT
                     FSSDVTEERP"
     gene            complement(336..1544)
                     /locus_tag="VFJ72_05405"
     CDS             complement(336..1544)
                     /locus_tag="VFJ72_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7478937.1"
                     /translation="MLSLRYKKSVPRYLLMRVGSKRFKSLETSRLAPLQLEDVPEPKL
                     PTPEWVRVKPLLSGICGSDLGTLSSENSPYFSPITSPPFVMGHEVVGVVAESNSGFER
                     GERVVLEPALGCAVRGIEPLCPFCVSGRHALCLNVTKGSISPGIQTGFCRDTGGGWSQ
                     RTLVAHPSQLHRVPDSVPDEAAVAIEPLACAVHAALKVSPGPDETTLVIGAGSVGLFT
                     VAAVRRLTEAGRIICVAKHERQRQEALRLGADEVVRPKEVYTTLPNMLGTDVYKPELG
                     KPVVMGGADKVFECVGSAGTMEDAVRLTKPRGEVALVGMPGARSSLDLTALWYKEVDM
                     AGTYAYGIEDYRGERVSSFELALRIAPEINLETMVGPLFRLEEYREAIAAARSSGREG
                     HVKVAFGLRR"
     gene            complement(1546..3810)
                     /locus_tag="VFJ72_05410"
     CDS             complement(1546..3810)
                     /locus_tag="VFJ72_05410"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET7478938.1"
                     /translation="MRSVLSESYRGKTIFLTGGTGFLGTALVEKILRSLPDLGRLYLL
                     IRSSREKSAADRFEKDMLGSAAFRGLREKLGDGFEEYVTGKVRVLEGDVHAPSLGLGD
                     EALSELSREVDIVIHSAASVVFDAPLDAAVGSNVQGTLGLLKLARGWEKRPLFMHIST
                     SYVAGNREGLALEEPPGNRTPNGTTLDAKEEVLSLEAVVEEVDRASQERGLLRRFETE
                     ARRELGMVGEEEEVAERVDTLRRAWMRERLVERGTERAQELGWNDVYTFTKSLAERMV
                     VRERGDIPLVILRPAIIESSFREPYPGWIQGSRMADPIIMAFAKGLLREFPGDPETLV
                     DIVPVDHVVNAILAAAVRRPERPEVFQVASGERNPLRYRDLYDHVRGYFVENPLRDSG
                     GRPVSVPEWSFPGRKRVENGLKVELAGLKIAGKIAARMPDGHMASDARQRIARAEKRA
                     RMSLYYSRIYGAYANMKGTFSTERTRALYESLPTEDRQEFPFDITRLDWRKWLQETHL
                     PALTTRPNRKKRKKAADEKPGEVAAIFDVDGTLVGSNVVSYFAWLRMRELPAVARPFW
                     LAWFLTKVPYYWGLDQISRAHFNRAFYKNYAGWKPSRAKHLGHESFAGFTLDRMFPDA
                     LQQLRDHKAAGHRVVLLSGALDFLLEPMKDLVDDVLCATLAQEDGVYTGELSGAPVAG
                     DARARMLASFARRRNLDLARSYAYADSISDLPMLEAVGNPVAVNPDRRLAAAAKERGW
                     QTREWGKNRVAKKI"
     gene            complement(3791..4402)
                     /gene="plsY"
                     /locus_tag="VFJ72_05415"
     CDS             complement(3791..4402)
                     /gene="plsY"
                     /locus_tag="VFJ72_05415"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET7478939.1"
                     /translation="MVTVFLVLLGYVLGSVPTGYLVGRAFGVDVRSVGSGNIGTANVL
                     RAAGKWPAVFTLAGDMAKGLVPVVVARISTDNVWVHALVAFAAVVGHCWPVFLKFKGG
                     KAVATGAGTSIGLAPLVGLGLFAFWWAVVLASRYTSLGAIAVMVVSPFVFILTGQPLA
                     YVLYTIVGGALVLWRHRENARALLNGTERKVGQRAESGEKRVK"
BASE COUNT          743 a         1594 c         1402 g          703 t
ORIGIN      
        1 ggggacatgc tcggcgcggc cagcctcggc ctgcacgtcc tacagatgag cggccagagc
       61 gagatagacg agatgctgaa cctcgtcacc acccgcgccg cccgaacttt aggcatagaa
      121 gaccgctacg gcatcgaaga gggcaagccc gccagcctcg tcatcttcga cgccaaaacc
      181 cccttcgaca ccctgcggct caacccggcc cgccttcacg ttataaaaga cggcagaacg
      241 gtcgccgcca ccgagcccgc ccgaaccacc ttgcggcgcg aaggcgggcg ctcggagacg
      301 gtcacgttct cttcggacgt tacggaggag cgtccctaac gacgcaggcc gaaggcgacc
      361 ttcacgtgcc cctcgcgccc ggaactccgg gcggctgcga tggcctcgcg atactcctcc
      421 aggcggaaga gcggaccgac cattgtctca agatttatct ccggcgcgat ccgcagcgcc
      481 agctcgaaac tgctcacccg ctcgcccctg tagtcctcaa tcccgtaggc gtacgtcccg
      541 gccatgtcga cttccttgta ccagagcgcc gtcaggtcca ggctgctcct cgcccccggc
      601 atcccgacga gcgccacctc tccgcgaggt ttcgtcagac gcaccgcgtc ctccatcgtc
      661 cccgcggagc ccacgcactc gaacacctta tccgccccgc ccatcacgac cggcttgcca
      721 agctccggtt tgtaaacgtc cgtgccgagc atgttcggga gcgtggtgta gacctccttc
      781 gggcgcacta cctcgtccgc cccgagccgc agggcctcct gccgctgccg ctcgtgtttg
      841 gcgacgcaga tgatgcgccc cgcctccgtc agccgccgca ccgccgcgac cgtgaacaac
      901 ccgacgctac cggccccgat gacgagcgtg gtttcgtccg gccccggact caccttgagc
      961 gcggcgtgaa cagcgcaggc gaggggttct atggcgacgg cggcctcgtc cggcacgctg
     1021 tcggggacgc ggtgtagctg cgaggggtgg gcgacgagcg tccgttgcga ccagccgcct
     1081 cccgtgtcgc ggcagaagcc ggtctggatg ccgggcgaga tgctgccctt cgtcacgttc
     1141 agacacaggg catggcgtcc cgagacgcag aaaggacaca gcggttcgat gccccgcacg
     1201 gcgcagccca gcgcgggctc cagcaccacg cgctcgccac gctcgaagcc ggagtttgat
     1261 tccgcgacga cgcccacgac ctcgtggccc atcacgaagg gtggggatgt gatcggcgaa
     1321 aagtacggcg agttctcgga agagagggtc cccaggtccg agccgcagat acctgagagc
     1381 agcggcttta cgcgcaccca ctccggcgtc gggagcttcg gttcggggac gtcctctagc
     1441 tgaagcggcg cgagccggct ggtctccaga ctcttaaagc gtttcgagcc cacacgcatg
     1501 aggaggtagc gtgggacgga cttcttgtag cggagtgaga gcaaactaga tctttttggc
     1561 gacgcggttc ttgccccact cgcgggtctg ccaaccccgc tctttcgccg ccgcggcgag
     1621 ccggcggtcg gggttgacgg cgaccgggtt gccgacggcc tccagcatcg gcaggtcgga
     1681 gattgagtcg gcgtaagcgt aggagcgcgc gagatcgagg ttgcgccggc gcgcgaaaga
     1741 ggcgagcatc cgggcgcggg cgtctcccgc caccggcgcg ccggagagct ccccggtgta
     1801 gacgccgtcc tcctgggcga gcgtggcgca gagcacgtcg tctaccaggt ccttcatcgg
     1861 ctccagcagg aagtccaggg cgccggagag caggactacg cggtggccgg cggccttgtg
     1921 gtcgcggagt tgctggagcg cgtccgggaa catccggtcg agtgtgaagc cggcgaagga
     1981 ttcgtggccg aggtgcttcg cccgcgaggg cttccagccg gcgtagttct tgtagaaagc
     2041 gcggttgaag tgggcgcggc tgatctggtc aagcccccag tagtacggca ctttggtcaa
     2101 gaaccacgcg agccagaacg gccgcgcaac ggccggcaac tcccgcatcc tcagccacgc
     2161 gaagtacgag acgacgttcg accctacgag cgtcccgtcc acgtcgaaga tcgccgccac
     2221 ctcccccggc ttctcgtccg ccgccttctt ccgcttcttg cggttcgggc gcgtggtcag
     2281 cgccggcagg tgcgtctcct gcagccactt cctccagtcg agccgcgtga tgtcgaaggg
     2341 gaactcctgg cggtcctcgg tcggcaggga ctcgtagagc gcccgtgtgc gctcggtgga
     2401 gaaggtgccc ttcatgttcg cgtaggcgcc gtagatgcgg ctgtagtaga ggctcatccg
     2461 ggcgcgtttc tcggcccgcg cgatcctctg ccgcgcgtcc gaggccatgt gaccgtccgg
     2521 catccgggcg gcgatcttgc cggcgatctt gagccccgcc agctccacct tgagcccgtt
     2581 ctcgaccctc ttgcggccgg ggaagctcca ctccggaacg gaaacgggac gcccgccgga
     2641 gtcgcgcagc gggttctcga cgaagtagcc gcggacgtgg tcgtacaggt cgcggtagcg
     2701 cagcgggttg cgttccccgg acgccacctg gaagacctcc ggccgctcgg ggcggcggac
     2761 ggcggcggcg agtatagcgt ttacgacgtg atccaccggg acgatgtcca ctaacgtctc
     2821 ggggtcgccg ggaaactccc gcagcagacc cttcgcgaac gccatgatta tcggatcggc
     2881 catcctagag ccctggatcc aaccagggta aggctcccgg aagctgctct ctatgatcgc
     2941 cggccgcagt atgacgagcg gaatatcccc ccgctcccgc accaccatcc gctccgcgag
     3001 cgacttggta aaggtgtaga cgtcgttcca ccccagctcc tgcgcccgct cggtcccccg
     3061 ctccacgagg cgctcccgca tccaggcccg ccgcagcgtg tcaacccgct cggcgacctc
     3121 ctcctcctcg ccgaccatcc cgagctccct gcgcgcctcg gtctcgaacc gccgcagcag
     3181 cccgcgctcc tgagaagccc ggtccacctc ctccaccacg gcctccaggc tcaggacctc
     3241 ttctttggcg tcgagcgtcg tgccgttggg cgtcctgttt cccggcggct cctccagcgc
     3301 gagcccctcg cggttaccgg ccacgtagga cgtcgagatg tgcatgaata gcgggcgctt
     3361 ctcccagccc cgcgccagct tgaggagccc cagggttccc tgcacgttcg agccaactgc
     3421 cgcgtccagc ggcgcgtcga agacgaccga ggccgccgaa tgtatgacga tgtccacctc
     3481 gcgcgaaagc tccgacagcg cctcgtcgcc gagcccaagc gagggcgcgt ggacgtctcc
     3541 ttccaggacg cggaccttcc cggtaacgta ttcctcgaag ccgtcgccga gcttctcgcg
     3601 cagtccgcgg aaggcggcgg agccgagcat gtccttctcg aagcggtcgg cggcgctctt
     3661 ctcgcgggag gagcggatca gcaggtagag gcggccgagg tcggggaggg aacgaaggat
     3721 cttctcgacc agcgccgtcc cgaggaatcc cgtgccgccg gtcagaaaga tggtcttgcc
     3781 gcgataggac tcacttaaca cgcttctcac cactctccgc tctctgtccg accttgcgct
     3841 ccgttccgtt caagagcgcc cgcgcgttct cccggtgccg ccacaacacc agcgcccccc
     3901 cgacgatggt gtagaggacg tacgccagcg gctgtccggt gaggatgaag acgaacgggc
     3961 tcacgaccat caccgcgatc gcgccgagcg acgtatatcg gctcgcgagc accaccgccc
     4021 accagaacgc gaagagcccg agcccgacaa gcggcgcgag cccgatgctc gtcccggccc
     4081 ccgtcgctac cgccttgccg cccttgaact tcaggaatac cggccagcag tgcccgacca
     4141 cggccgcgaa agctaccagg gcatgcaccc acacgttatc cgtggagatc cgggcgacga
     4201 ctaccggcac caggcccttc gccatgtccc cggcgagcgt gaagacggcc ggccacttcc
     4261 ccgccgcccg caggacgttc gccgtcccga tgttcccgga accgacactc cgaacgtcca
     4321 cgccgaaggc gcgcccgacc agatatccgg tcggcaccga gcccagcacg tagccgagta
     4381 atacgaggaa taccgtcacc ataagcgaga gtatagctta acgggatgct cttcttacag
     4441 ca
//
LOCUS       DASSMB010000084        17072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1412446, whole genome shotgun sequence.
ACCESSION   DASSMB010000084 DASSMB010000000
VERSION     DASSMB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 17072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17072
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412446"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VFJ72_05420"
     CDS             <1..522
                     /locus_tag="VFJ72_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478940.1"
                     /translation="SAGKTGTTEEFADAWYVGFIPQLSTSVWVGYPGERRSMVNINGL
                     TEINGENYPLDIFSLYMQNAVSLYPVQQFDVPSPYLNLQIKKSGRAATALETTGKEDS
                     KLEGTVFDPTPRPQQVQPTPQQVQPTPQQAQPTPQQAQPTPQPSNSGSGGGGGGGGGG
                     FPADGVFDGSIFN"
     gene            complement(564..1388)
                     /locus_tag="VFJ72_05425"
     CDS             complement(564..1388)
                     /locus_tag="VFJ72_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HET7478941.1"
                     /translation="MNTSGSTGNTLDTSKELHEFVCDLARRAGELQLSRHESPGEIRE
                     KGPKDYVTEVDLLCEELMISAIEEHYPEDAILSEERGGEISASGRSWLLDPVDGTANF
                     ARSNPLFCACISVIENDTVTHAAVAAPRLGDLYHARLGGGAYRENGGVSTPLEVSATR
                     ELDDAFVGADISFMGAMREDGRKGTDEVFAASWQLRALGSAGIRGAWIAAGYLDASIG
                     TRNTAWDYAPTVLLVSEAGGRATDITGEPWTPASQGLVASNGDPLHAEILGILNKR"
     gene            complement(1388..2491)
                     /locus_tag="VFJ72_05430"
     CDS             complement(1388..2491)
                     /locus_tag="VFJ72_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7478942.1"
                     /translation="MSTENTTLLAERTDELLNRGRSQGFISTEEVADLLQEGDLSATE
                     VEEFYAALEEEEITLVENESAEATSPAVAAIAVTQAPETPAVQIAHAVATGDSIRMYL
                     AEIGRVKLLTHADEIRLAKGIARGCKRSKDRLVEANLRLVVSIAKKYRNRGVSFLDLI
                     QEGNLGLIRAAEKFDHTKGYKFSTYATWWIRQAITRAIADKGRTIRIPVHMVEKVNKF
                     HRTHRRMTQTLGREPLDEEIARELDIPVEEILRLREISQRAISLETPVGEEDSSHLGD
                     FLEDATTVTPTEAVSESLLKLHLREALDELPERERQIIELRFGMKDDRPRTLEEVGRE
                     FDITRERVRQIQMKTLNLLREQRRTQNLREYLR"
     gene            2753..4246
                     /locus_tag="VFJ72_05435"
     CDS             2753..4246
                     /locus_tag="VFJ72_05435"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7478943.1"
                     /translation="MILERRELERTLGARVPTGVDRIDGVTHDSRQVRPGFAFVAVPG
                     FRRDGAEFVPDAVRGGAALIVSERPLTGVPSGVPAVVVPDARVALAALACAVQGNPSA
                     ALEVYGVTGTNGKTTTSYALYALLAAAFGKEKCGLMTTAEVVYGGERRPADRTTPEAV
                     EVQGTLAEMLEGGVRRVVMEVSSHGVELRRVSGTRFCGAIFTNLTRDHLDLHGTMEAY
                     YAAKRELFYWADGPKLANAEDSWGRRLAGEVEGVKTFGGTQEADYRVTDVRDTREGTA
                     FALRHQGGVTEIQTPLLGPYNVLNVAGAAALALEIGVEGETLASAAMEMEQVPGRFER
                     VAKREEHGFEVIVDYAHTDVGLEAVLQVARGAANAAANAAANGHPGRVICVFGAAGDR
                     DGAKRPLMGRVASGLADLGIVTTDDAYSEDPEKIAAEVAAGADPERTEVILDRQAAIR
                     RALECAREGDVVVIAGKGHERVQHLPEGDVPFHDATVVEELLGTLGR"
     gene            4253..4513
                     /locus_tag="VFJ72_05440"
     CDS             4253..4513
                     /locus_tag="VFJ72_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET7478944.1"
                     /translation="MQKQNRAEVLRRLKSVEGHVRGVAKMVEEGDVTYAEVVQQTTAI
                     FSAIRRINTVILKDFVEERAERDGASEDLIRDLFKAIDRVMK"
     gene            4516..4788
                     /locus_tag="VFJ72_05445"
     CDS             4516..4788
                     /locus_tag="VFJ72_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478945.1"
                     /translation="MNHQMRLLYTLIAVIPIVVAGAGLAAFLLLRAGYGPLVWGVLPF
                     LAMLALAGIIGAVLGRAATNRRVPRDDRPPRDTDRDTDQKRADDDV"
     gene            4808..5092
                     /locus_tag="VFJ72_05450"
     CDS             4808..5092
                     /locus_tag="VFJ72_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478946.1"
                     /translation="MEDRRAPGIDRLEQQVDELERLTDTLGEVPDEEVVAVLARAVEL
                     LQEINSGIEAGLASSQQGAQDVSALLERVNLGPFDEALEEIEQKSGEPGA"
     gene            5076..5609
                     /gene="pyrE"
                     /locus_tag="VFJ72_05455"
     CDS             5076..5609
                     /gene="pyrE"
                     /locus_tag="VFJ72_05455"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET7478947.1"
                     /translation="MSLVPDLAARVREAALLEGDFVLSSGQRSSFYVDKYRFSTDPVL
                     LRDIAASLANRLPQDVTRLAGVELGAVPLVVAASLETGLPYIIARKAAKEYGSSAGSA
                     FEGEFERGERVALIEDVVTTGTQAVAAARRLVEAGAEVAGIIAVLDRREVREASLGEF
                     GFDALLTIEDLRVETGD"
     gene            5693..5965
                     /locus_tag="VFJ72_05460"
     CDS             5693..5965
                     /locus_tag="VFJ72_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7478948.1"
                     /translation="MNKTELIQKIADEGNGSKSDAQKFFDAFTGVITDALKKDDVVQI
                     TGFGKFYVQKREAREGINPQTKQKINIPASKVPKFTAGNALKDSLK"
     gene            6029..6874
                     /gene="pyrF"
                     /locus_tag="VFJ72_05465"
     CDS             6029..6874
                     /gene="pyrF"
                     /locus_tag="VFJ72_05465"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR02127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET7478949.1"
                     /translation="MQALVDSARRRQSVLIAGLDPIPNRLPPEVLDRGLSEARATGEF
                     CREILEAVDPYVAAVKLQSAYFERLGPAGMQVYADLIGQADSLGLPVIADVKRGDIGP
                     VAAAYAEAHLLVYGASCVTVNPYMGIDAVRPFLEQARRLGRGGGVFALVATSNPSAGE
                     IQNAGEPPLYEVAARLVRQVGGGDGEYPDAGAVVGVTHPEVGRRVRELLPDTLFLAPG
                     YGAQGGGAEGVRSLLDARNAGVLVNSSRGIIYAHEGSGLDYREAARQAARHARDELRG
                     TGARF"
     gene            complement(7519..8049)
                     /locus_tag="VFJ72_05470"
     CDS             complement(7519..8049)
                     /locus_tag="VFJ72_05470"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7478950.1"
                     /translation="MRRLLVVVVLVLAAAVAYRSFGSAETGTGTLTLAQPAPNVGQKV
                     PAFTATGIDGEDFDLSKKGAYVLTYWSTLNKSSSDARPDFARLADEYGSRGVSFAAVY
                     VNGAPTEDESAPYVVIQDSHGRLASLYNVKRVPRLFLIRNGRVELVQNGFYDGSTRAL
                     RAKLDELMDERAETSR"
     gene            8167..9378
                     /locus_tag="VFJ72_05475"
     CDS             8167..9378
                     /locus_tag="VFJ72_05475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HET7478951.1"
                     /translation="MNDTSKTLVLALVLALTLLFTVKVADARTQETTESTAALEKAQQ
                     EAPKPVTKPAAPDVAAKSWALVDLQSGEYLAGEDASKELPMASTTKIMVALVALKDAD
                     LDDKVIVSKKAASFATQIYSNVGLFPGDELTVRDLLKATLISSGDDAVYALAEHLGGG
                     SAKRFVEEMNREAKTLGLKDTHFENPSGLDARGHHTSARDLATMTRAAMKYPVFREMV
                     GTSYATIQTQDRQIPLTNTNDLLATYAPATGVKTGTTPAAGSCLVASAADGNESYVAV
                     FLDSKEDRFAAAARTLDYGFATYDREDLVKKGDRYAKVEVPYRRGETVSAVAKGAVEG
                     LVYKGADVEREPKIMKDLPDSARRGTKMGEIVVKVDGKRVGEAALVARKGYEEASIWQ
                     RVWYTVGGIFE"
     gene            9401..10360
                     /locus_tag="VFJ72_05480"
     CDS             9401..10360
                     /locus_tag="VFJ72_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HET7478952.1"
                     /translation="MILTVTLNAAVARTLVVPSLTLGHRHRAPESIALAGGKGINVAR
                     ALRSLGVPVLATGFAGGRNGDTIRDGLSEGAIPFDLVEIEGHSRTSTAIIDPMAGTQT
                     EINEYGPEVSQAEAREFSRRLEHLMEYATAVVFAGSLPPNFEESFLVGLIQRSRERGL
                     FTVVDSTPTVLRAALKASPSLVSPNQHESESVAGFDFIEEEDFVRGLNRLIELGAESA
                     CITSPEGHSYFTSEEGIVSALAPRVEAVSPIGSGDAYLAGLLTGLLHRDLPPVDAVRL
                     AAGCAAANAETLGAGVLDPRRAEELAEDVQIELHNRSFQSGRV"
     gene            10477..10788
                     /gene="trxA"
                     /locus_tag="VFJ72_05485"
     CDS             10477..10788
                     /gene="trxA"
                     /locus_tag="VFJ72_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET7478953.1"
                     /translation="MAVGEVTDATFEGEVLQSEQPVIVDFWAPWCVPCRRISPILEEI
                     SESRDDVRFVKLNVDDNPATAMNYQISSIPTITRFEGGQVTQQAVGALPKNQLSAQLG
                     L"
     gene            10895..11185
                     /gene="groES"
                     /locus_tag="VFJ72_05490"
     CDS             10895..11185
                     /gene="groES"
                     /locus_tag="VFJ72_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET7478954.1"
                     /translation="MKFKPLGERALVQLVEREEKTASGIVLPDTAKDKPQTAEVVSVG
                     EGKLDDKGQRVPLEVSQGDVIVFAKYSGTEIKLDGEDYMILDADDILGVVED"
     gene            11335..12405
                     /locus_tag="VFJ72_05495"
     CDS             11335..12405
                     /locus_tag="VFJ72_05495"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7478955.1"
                     /translation="MRTLRRRDRGREVVDLQTRLQALGFDLGNRGIDGVFGPRTELAV
                     KAFQQSIGILADGLVGPISWREIVEAGYRPGGRLLYVRQPPFRGADVVELQRMLNDLG
                     FDPGAVNGLFDQRTARATHDFQKNAGLQADGVVDGAVFKVLGTYAGQTLGTHQLPDKH
                     GGYFPEDLFSGTMVVDAAHGGRDTGYVAKGGLSEADLNLAVAQELAQILPASEVLLTR
                     SGDDEVSPEDRAFLANSSGAKMVISIHHNHHRTQTANGTAAYYFERLGYQSHRGNMAA
                     TYIQRGVSKALGTCDIGEFGRSYDILRETNIPAVMLEPLHLTNPGELKLASDPYYPTT
                     VAEAVVGALELYAGRDAAFIAV"
     gene            12466..12539
                     /locus_tag="VFJ72_05500"
     tRNA            12466..12539
                     /locus_tag="VFJ72_05500"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12500..12502,aa:Pro,seq:cgg)
     gene            complement(12575..13438)
                     /locus_tag="VFJ72_05505"
     CDS             complement(12575..13438)
                     /locus_tag="VFJ72_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478956.1"
                     /translation="MRLRNTLPLTLLLLASFFSNSCGGTSSTLYAGRSLDAPAVYRVS
                     VTAESSFSGAASDLRGASDLTGAFQVTPISESSVEVKVLYLAVNVRDGEGEPVALDLK
                     DLAGKKAVVKMSPPGVVSEVRGDKALLEAPVPLISVREVIWSLFPPLPQDNVGEDDTW
                     TGDMPIPFANLGGPPHRMRYVMQSMGSGGKLGWIQGYELSVRPRSFTADTPGGSVSGE
                     GDLDVEFEGELAARQGYESIERTAKFDTNYIRLQDGGSYANGKLHMQSLMKVERLSSA
                     EQFGLDIGTKN"
     gene            complement(13435..15327)
                     /locus_tag="VFJ72_05510"
     CDS             complement(13435..15327)
                     /locus_tag="VFJ72_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7478957.1"
                     /translation="MKVLIARDIVKYHGGDLKVLAGASLAVEAGEKIGLVGRNGAGKT
                     TLLEILAGNSEADSGGVERVNGAKVGLTSQSLYAGERGTHSIEEEIISAFAPLISREK
                     ELKAMESQLSENPSDALLERYGRLQSEFERDGGYEYRARAASTLSGLGFSPEDWKRPV
                     GSFSGGEQSRVALARLLLEEPDLVLLDEPTNHLDLRAIEWLETFIKNAKSAVLVVSHD
                     RYFLDAISDSIVELEDGKLVRYTGNYTKYVEEKKARDEQLARKAKANAERRAQLERFI
                     EKNRAKNTKATQAKSKQKLLDRMDKIEGPKNGSKRMRVDLGGETSRAGRVVLEMEDVR
                     YAHPDADGPLLDDLEIVIERRERVALLGPNGTGKSTIMRLATGELEPQGGIVRLGNNV
                     EPAYQDQQLARLEGSKTVLQEVMDATGFDAPEARDLLGAFLFSGDDAFKKVSSLSGGE
                     KSRLSLAEIVVSGANLLLLDEPTNNLDIPAREALEEALAGYRGTLFFISHDRYFLRKL
                     ATRIVELDNRKLTNYLGGYDYYRAHRRLDVKEGVRRPRRRVRPGMSKSESILATRLVA
                     VEGEIDATERRVARLEKELATSELYSDGKRSREVVLEHRQLKSILDGLYTDWTQLLEE
                     AEEAGL"
     gene            15326..16120
                     /locus_tag="VFJ72_05515"
     CDS             15326..16120
                     /locus_tag="VFJ72_05515"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7478958.1"
                     /translation="MTQGLYRQDPLTPKEREEFEADLITFCDRELERLGEIRDLHVLY
                     AGGVSPLWLEGLSQRIGPKGSLTALEVDGERAAAARETLREMDLSAPVEIVEGDAFDP
                     PFEPHSLDLVYSAGLFHELDVRERPAGGALAALASVVRPGGRVATSDFVDSTPAAQIE
                     DEAIERELARAASGAVLYGIGPPERLVELHEQVLSGVRWISSPPFVIRHLDRVALAQD
                     PFEGWDLPDHTVRELRRRYDVLRARVRREGYTRPTTLYVEGIVSGR"
     gene            complement(16149..16589)
                     /gene="dtd"
                     /locus_tag="VFJ72_05520"
     CDS             complement(16149..16589)
                     /gene="dtd"
                     /locus_tag="VFJ72_05520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET7478959.1"
                     /translation="MRIVLQRVRSASVTVEDEEVSSIGPGLLLLVGVAEGDGEAHADW
                     LAEKVAGLRIFNDEDGKMNLSALDTGGEVLAVSQFTLLADTRKGKRPSFIKAAAPEKA
                     EPLFDYFCERLRAAGVEPVKTGRFGAMMDVALVNDGPVTIILER"
     gene            complement(16595..16981)
                     /locus_tag="VFJ72_05525"
     CDS             complement(16595..16981)
                     /locus_tag="VFJ72_05525"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HET7478960.1"
                     /translation="MEPAFSRLFTVAEANALLPTLKELLDDLALHLAAVREKAPHMEP
                     ILRAAGANGGGRMGSEYGVEAYNLHLAIERIRELGVIVKDLDMGLLDFPHERDGRVVF
                     LCWHPPEERVEFWHEIETGYAGRKPL"
BASE COUNT         2929 a         5292 c         5797 g         3054 t
ORIGIN      
        1 tcggcgggca agaccggcac gaccgaggag ttcgcggatg cctggtacgt gggctttatc
       61 cctcagctat ctacaagcgt ctgggtggga tatccgggcg agcggcgctc gatggtcaac
      121 ataaacggcc ttacggagat caacggcgag aactacccgc tcgacatctt ctcgctctat
      181 atgcagaatg ccgtgtcgct ttatccggtc cagcaattcg acgtcccgag cccgtacctc
      241 aacctccaga tcaagaagag cggccgcgcc gccaccgctc ttgagacgac cgggaaagaa
      301 gactccaaac tggagggaac ggtcttcgat ccaaccccgc ggccgcagca agtccagccg
      361 acgccgcaac aagtacagcc cacaccgcaa caggcacagc cgacgccgca acaggcacag
      421 ccgacgccgc agccgtcaaa ttcgggtagt ggcggtggag gcggtggtgg tggcggaggc
      481 ttcccggcag acggagtttt cgacggcagt atcttcaatt aacgtcggga gatcctttcc
      541 cgatctcttc cggcgcgggt tgactatctc ttgttgagaa tacccagtat ctccgcgtgg
      601 agcggatctc cgttgcttgc caccagaccc tgcgaggcgg gggtccacgg ctcgccggtt
      661 atatcggtgg cgcggcctcc ggcctcggag acgagcagga cggtgggcgc gtagtcccag
      721 gcggtgttgc gggtcccgat agaggcgtcg aggtagccgg cggcgatcca ggctccacgt
      781 atccccgcgc tgccgagcgc ccggagctgc caggaggcgg cgaagacctc gtcggtgccc
      841 ttcctgccgt cctcgcgcat cgcgcccatg aacgagatgt ccgcgccgac gaaggcgtcg
      901 tccaactccc gcgtcgcgct gacttcgaga ggggtgctga cgccaccgtt ctcccggtag
      961 gcgccgccgc cgagtcgggc gtggtagagg tcgccgagcc ggggggcggc gacggcggcg
     1021 tgcgtgacgg tgtcgttctc gatcacggag atacaggcgc agaagagcgg gttgctcctg
     1081 gcgaagttcg ccgtgccgtc cacgggatcg aggagccagg accggccgga ggctgagatc
     1141 tcaccgcccc gctcctcgga gaggatggcg tcctccgggt agtgttcctc tatcgccgag
     1201 atcattaact cctcgcacag gaggtctacc tcggttacgt agtccttggg gcccttctcg
     1261 cggatctcac cgggactctc gtggcgcgag agctgcaact cgcccgcccg gcgcgcaagg
     1321 tcgcagacga actcgtgtag ctctttcgaa gtatccaggg tgttgccggt gctaccggaa
     1381 gtgttcacta ccggagatat tcacgcaggt tctgggtgcg gcgctgctcg cgcagcaggt
     1441 tgagggtctt catctggatc tggcggacgc gctcgcgggt gatgtcgaac tcgcggccga
     1501 cttcctccag ggtgcgggga cggtcgtcct tcatcccgaa gcgcagctcg atgatctggc
     1561 gctcccgctc cggcaactcg tcgagcgcct cgcgcaggtg cagcttcaag agagactcgg
     1621 agacggcctc ggtcggggta acggtcgtgg cgtcctccag gaaatcgccg aggtgggagg
     1681 aatcctcctc gcccaccggc gtctccaggc tgatggcccg ctggctgatc tctcgcaggc
     1741 gcaggatctc ctctaccggg atgtccagct cgcgggctat ctcctcatcg aggggctcgc
     1801 gtccgagggt ctgggtcata cggcggtggg tgcggtggaa cttgttgacc ttctcgacca
     1861 tatggaccgg gatgcggatc gtgcgtccct tgtcggctat cgctctggtg atggcctgac
     1921 ggatccacca cgtcgcgtag gtcgagaact tgtagccctt ggtgtggtcg aacttctccg
     1981 ccgcccgtat gaggccgagg ttgccctcct gtatgaggtc caggaaagac actccgcggt
     2041 tgcggtactt cttggcgatg gagacgacga gtctcaggtt cgcctcgacc agccggtcct
     2101 tcgaccgctt gcagccgcgg gcgataccct tggccagccg gatctcatcg gcgtgcgtga
     2161 ggagcttgac gcgcccgatc tcggccaggt acatgcggat cgaatcgccc gtcgcgacgg
     2221 cgtgagctat ctggaccgcc ggcgtctccg gcgcctgcgt cacggctata gcggcgaccg
     2281 ccggagaggt tgcctccgcg gactcgttct cgacgagcgt gatctcctct tcctcaagcg
     2341 cggcgtagaa ctcttcgact tccgtcgccg ataggtcgcc ctcctgcaga aggtccgcga
     2401 cctcctccgt actgatgaaa ccctgactcc gcccgcgatt caagagctcg tccgtgcgct
     2461 ccgccagcaa cgtggtgttc tcggtcgaca tctatctcct ctcctgagac aaggccatcg
     2521 tgacctgcga gttcaacatc cgaggtgccc ggtttgtaaa gttcctgtta aaaatgactc
     2581 gcgtagacta acacaccata tatggacagt caccgcgatt ctggccaatt tagtgtgttt
     2641 tatcttactt ttcccaggta tcaatctcca cccgtcatat ttcggcgatg tttcgatgtt
     2701 gttgcggccg gtttgccgag tcgcttgccc ggctcttgct acactacgcg gtgtgattct
     2761 ggagcggcga gagcttgagc ggaccctggg agcgcgcgtg ccgaccggcg tggatcgtat
     2821 agatggagta acccacgatt cgcggcaggt ccggccgggc ttcgctttcg tggcggtccc
     2881 cggcttccgg cgcgacgggg ccgagttcgt cccggacgca gtgcgcggcg gcgcggcact
     2941 catcgtttcg gagcgtcctc tgaccggagt tccttctggc gtcccggcgg tcgtcgtccc
     3001 ggatgctcgc gtcgcgctgg ccgctctcgc gtgcgccgtg caggggaatc cctcggcggc
     3061 tctggaggtc tatggcgtaa ccggcaccaa cggcaagacc acgacctcct acgcgctcta
     3121 cgcgctcctc gcggcggcct ttgggaagga gaaatgcggc ctgatgacca cggcggaggt
     3181 cgtgtacggt ggggagcggc gcccggccga ccgaactacg ccggaggccg tcgaggtgca
     3241 ggggacgctc gcggagatgc tggagggcgg cgttcggagg gtcgtcatgg aagtctcctc
     3301 ccacggcgtc gagctcagga gagtctccgg gacgcggttc tgcggcgcta tctttaccaa
     3361 cctgacccgc gaccacctcg acctgcacgg cacgatggag gcgtactatg cggccaagcg
     3421 cgagcttttc tactgggccg acgggccgaa gctcgccaac gccgaggact cctggggacg
     3481 gaggctcgcc ggcgaggtcg agggcgtaaa gaccttcggc gggacccagg aagccgacta
     3541 ccgcgttacg gacgtgcgcg acactcggga ggggacagcc ttcgcgctgc gccatcaggg
     3601 gggtgttacg gagattcaga ccccgctatt gggtccgtac aacgtgctca acgtcgccgg
     3661 ggccgcggcg ctggcgctgg agatcggggt ggaaggagag accctggcga gcgctgccat
     3721 ggagatggag caggtgcccg gacggttcga gcgggtggcg aagcgcgagg agcacgggtt
     3781 cgaggtcatc gtggactatg cgcacaccga cgtagggctc gaagcggtgc tacaggtggc
     3841 gcgcggagct gcgaacgcag cggcgaacgc ggcggcgaac ggacaccccg gacgggttat
     3901 ctgcgtcttc ggggcggcgg gggacaggga cggggccaag cgtccgctga tgggcagggt
     3961 agcgtccggc ctcgcggatc tcggcatcgt cacgaccgac gacgcgtact cggaggaccc
     4021 ggagaagatc gcggccgagg tggcggcggg ggcggacccg gagcgcaccg aggtcatcct
     4081 ggaccggcag gcggcgatcc ggcgggcgct cgaatgcgcg cgggagggtg atgtggtagt
     4141 catagccggt aagggacacg agagggtcca gcacctaccg gagggagacg tacccttcca
     4201 cgacgccacg gttgtcgagg agctcttggg gacgctgggg aggtagagcg ggttgcagaa
     4261 gcagaacagg gccgaggtgc taaggcgtct caagagcgtg gaggggcacg tgcggggtgt
     4321 cgccaagatg gtagaggagg gggatgtcac ctacgcggag gtagtgcagc agacgacggc
     4381 gatcttctcg gcgatccggc gcatcaatac cgtgatactc aaggacttcg tcgaggagcg
     4441 cgccgagcgg gacggggctt cggaagattt gatccgggac ctcttcaagg ccatcgaccg
     4501 ggtgatgaag tagcgatgaa ccaccagatg aggctgctct acaccctgat cgccgtgatc
     4561 ccaatcgtcg tcgcgggcgc gggcctcgcc gccttcttgc tgctgcgcgc cggatacggg
     4621 ccgctggtct ggggcgtgtt gccgttcctg gcgatgctgg cgctggccgg aattataggc
     4681 gccgtcctgg gacgcgcggc gaccaaccgg cgcgttccgc gggatgaccg tcctccgcgg
     4741 gatacggatc gagacacgga tcaaaagcga gcggacgatg atgtatagtc cgggacttat
     4801 ggaacccgtg gaagacagac gcgcgcccgg tatagaccgg ctggagcagc aggtggacga
     4861 gctggagagg ctcacggaca cattgggcga ggtgccggac gaagaggtcg ttgcagtgct
     4921 cgcccgggcg gtggagttgc tccaggagat caacagcggc atcgaggctg gtcttgcgtc
     4981 ctctcagcag ggcgctcagg acgtgagcgc gctcctggag cgggtaaacc tcgggccgtt
     5041 cgacgaggct ctggaggaga tcgagcagaa gtccggtgag cctggtgcct gacctcgctg
     5101 cccgcgtccg ggaggcggcg ctcctcgaag gggacttcgt gctctccagc ggccagcgca
     5161 gcagcttcta cgtggacaag taccgcttct cgaccgatcc ggtcctcctg cgcgacatcg
     5221 ccgcctctct cgctaaccgg ctgccgcagg acgttacgcg gcttgccggg gtggaactcg
     5281 gagccgtgcc gctcgtcgtg gcggcttcgc tagagaccgg gctgccgtac attatcgccc
     5341 gcaaggcggc aaaggagtac ggaagctccg ccgggagcgc cttcgaagga gagttcgaga
     5401 ggggtgagag ggtggccctg atcgaggacg tggtcaccac cggaacgcag gccgtggccg
     5461 ccgccaggag gctcgtagag gccggagcgg aggtcgccgg gatcatcgcc gtcctcgacc
     5521 ggcgggaggt ccgggaagca tcattgggag agttcggttt cgacgcgctg cttacgattg
     5581 aggatttgcg ggtagaaacc ggcgattgag gccactatat atctctggta tagtcatcgg
     5641 aatgtatgta gggtaacaag gagtgtaagc tgtagatgga ggtagaggga cagtgaataa
     5701 gactgagttg atccagaaga tcgccgatga gggaaacggg tccaagagcg acgcgcagaa
     5761 gttcttcgat gcgtttaccg gggttatcac cgacgcgctc aagaaggacg acgtggtcca
     5821 gatcacgggc ttcggcaagt tctatgtgca gaagcgcgaa gcgcgggagg gcatcaaccc
     5881 gcagacgaag cagaagatca acattcccgc gtccaaggta ccgaagttca cggccgggaa
     5941 cgcgctgaaa gattccctca agtagtcacg gatattctgt ccttggtgcc gcggatattc
     6001 tgtccgcggc atttcttctg cttgtagtat gcaagctctc gtagattccg cccgccgccg
     6061 gcagagtgtg ctgatcgccg gcctcgaccc gatcccgaac cggctgccgc cggaggtcct
     6121 tgaccggggt ttgtccgagg cccgcgcgac cggagagttc tgccgggaga tcctggaggc
     6181 cgtcgaccct tacgtcgcgg cggtgaagct gcagtcagct tacttcgagc ggctcggtcc
     6241 ggccgggatg caggtctatg cggacctcat cgggcaggcg gactctcttg gcctgccggt
     6301 gatagccgac gtgaagcggg gtgatatagg cccggtcgcg gcggcttacg cggaggccca
     6361 cctcctcgtc tacggcgcct cgtgtgtgac cgtcaacccg tacatgggca tcgacgcggt
     6421 taggccgttt ctggagcagg cgcggcgatt gggcaggggt gggggcgtct tcgcgctcgt
     6481 ggcgacctcc aatccgtcgg ccggcgagat acagaacgcg ggagagccgc cgctctacga
     6541 ggtcgcggcc cgtctcgtcc ggcaggtcgg gggcggcgac ggcgagtacc cggacgccgg
     6601 agcggtcgtg ggcgttacgc atccggaggt agggcgcagg gtgcgggagc tgttgccaga
     6661 cacgctgttt ctggcgccgg gctacggagc gcagggcggc ggggccgagg gcgtgcggtc
     6721 gctactcgac gccaggaacg cgggcgtgct ggtcaatagc agccgcggca tcatttatgc
     6781 tcacgagggt tcgggactcg actacaggga ggcggctcgc caggccgccc gccacgcgcg
     6841 cgacgagctg cgcgggaccg gcgctcgctt ctaaggagac gcttccgtcg tccgctgcga
     6901 tcgcggaaga tcggtgggta gcagcgttgt ctgatcggac gtacacttct ggaggtacaa
     6961 gggtacttgc cgctgcgtat ccgagtttac gcatgcgcct tgagaagatc ctctactatc
     7021 agatcgaccc gcccaagctc tttaccgacc gggtctttac tgtgtcgtcg ggaccgccgc
     7081 tctaccactg ctctggaggc gccgtctccg acgagcgctc ctcgtcaagt tcgtgtagcc
     7141 cgttgccgct tcggtcttat cgtccggtac gcggacctcg aaccgtataa gaggtcgtcg
     7201 ctcgggcgtt acgtcgagcg tcacatggcc agggagatgc agaacctgat catgatgtat
     7261 ggcgcctgga cccggagatc ctggctggcg tatctcggtg ttcttgtcct gttccggtgg
     7321 ttgcgtggaa agctcttccc gcgagaccgg agcgtcagga ggtgaagagg ttgaccaagg
     7381 ccagtatcgt caccagtatc gttataagag cgatcagacc cccaaaaatc tgcactaggc
     7441 tctctcggct catgttctcc tcctggaagc tctgtaaggt tatgtctccg gcagtttatt
     7501 tctttgtgat gcgcgcggtc aacgggacgt ctccgcgcgc tcgtccatca actcgtctag
     7561 cttggcgcgg agggcgcgcg tgctcccgtc gtagaagccg ttttggacca actctaccct
     7621 gccgttgcgg atcaagaaca gcctcgggac gcgcttgacg ttgtagagcg aggcgagcct
     7681 gccgtgcgag tcctggatca cgacgtatgg ggcgctctcg tcttcggtcg gagcgccgtt
     7741 cacatagacg gcggcgaatg agactccccg gctaccgtac tcgtcggcca gccgcgcgaa
     7801 gtccgggcgg gcgtcgctgg aactcttgtt cagggtgctc cagtaggtca gcacgtaggc
     7861 gcccttcttt gacaggtcga agtcctctcc gtcaatgccc gtagccgtaa aagccgggac
     7921 cttctgtccc acgttcggtg cgggctgggc gagggtgagg gtgccggtgc cggtctcagc
     7981 gctgccgaac gaacggtagg ccacggccgc ggcgagcacg aggacgacga cgaccagcag
     8041 gcgcctcatg atcctcgcgg gtaaaggtcg gaagaacgat gcacaacacc cgtcagtata
     8101 cagcccgacc cgcatcccgt cccgccaaac gccagaccgt agagccccta taatcccctg
     8161 ggtctcttga atgacacgtc taaaacactc gttctcgcgc tcgtactcgc cctgacgctg
     8221 ctgttcacgg tgaaggttgc ggacgcccgg acccaggaga ctacggagag caccgccgcg
     8281 ctggagaagg cgcaacagga ggctccgaag ccggttacga aacccgcggc gccggatgtg
     8341 gcggcgaaat cctgggcgct ggtggacctt cagagcgggg agtatctggc gggagaggac
     8401 gcatccaaag agttgccaat ggcctcgacc accaagatca tggtcgcgct ggtggcgctc
     8461 aaggacgcgg acctcgacga caaagtcatc gtctccaaga aggccgcctc gttcgctacc
     8521 cagatctaca gcaacgtcgg cctgttccca ggtgacgagt tgactgtccg ggacctgttg
     8581 aaggcgaccc tgatctcctc gggagacgac gcggtgtatg cgctcgccga acacctcggc
     8641 ggcggcagcg cgaagcgttt cgtggaggag atgaaccggg aggcgaagac tctgggtctg
     8701 aaagacaccc acttcgagaa ccccagcggc ctcgacgccc gcggccacca caccagcgcc
     8761 cgcgacctcg ccaccatgac gcgggccgcc atgaaatatc ccgtgttccg ggagatggtc
     8821 ggcacgagct acgccacgat ccagactcag gatcgccaga taccgctcac gaacaccaac
     8881 gacctgctcg ccacctatgc gcccgcgacc ggcgtaaaga ccgggacgac gcccgcggcg
     8941 ggctcctgcc tcgtagcctc cgccgcggac ggcaacgagt catacgtcgc cgtcttcctg
     9001 gactccaaag aggatcgttt cgccgccgcc gcccgaaccc tggattacgg gtttgccaca
     9061 tacgaccgcg aagacctggt caagaaggga gaccggtacg cgaaggtcga agtgccctat
     9121 cgcaggggag agacagtctc cgccgtcgca aagggagccg tcgagggtct tgtgtacaag
     9181 ggggcggacg tggagcgcga accgaaaatc atgaaggacc tacccgattc ggcccggcgc
     9241 ggcacgaaga tgggagagat agtggtgaag gtggatggga agcgcgtggg ggaggccgcg
     9301 ctggttgcgc ggaagggcta cgaggaggct tcgatctggc agagggtgtg gtatacagtg
     9361 ggagggattt tcgagtaggg gatagagaaa gggactcgct gtgatcctga ccgttaccct
     9421 caacgctgct gtggctcgca cgctcgtcgt gccgagtctt acgctggggc atcgtcaccg
     9481 ggcgccggag agcatcgctc tcgcgggcgg aaagggcata aacgtggccc gcgctctgcg
     9541 gtctctgggc gtgccggtcc tcgcgacagg gttcgccggc gggcgcaacg gagataccat
     9601 ccgggatggg ctctcggagg gcgctatccc attcgacctc gtagagatcg aaggtcactc
     9661 ccgcacctcc acggccatca tagacccgat ggccgggacg cagaccgaga tcaacgagta
     9721 cggtcccgag gtgagccagg ccgaggcccg cgagttctcc cgccgcctgg agcacctgat
     9781 ggagtacgcc acggcggtcg tcttcgcggg gagcctgccg cccaacttcg aagagagctt
     9841 cctggtcggc ctgatccagc gctcccgcga gcgaggtctc ttcacggtcg tcgactcgac
     9901 ccccacggtc ctgcgagcgg cgctgaaggc gagtccctcg ctggtgagcc cgaaccaaca
     9961 cgagtcggag agcgtcgccg gcttcgattt catagaggaa gaggacttcg tgcggggact
    10021 caatcgcctc atagagttgg gcgccgagtc ggcctgcatc acctcgccgg agggacactc
    10081 gtacttcacg tcggaggagg gtatcgtttc cgccctggcg ccgcgggtcg aggccgtctc
    10141 gcccatcggg agcggcgacg cttacctggc gggactgctg acgggactgc tgcaccggga
    10201 cttgccgccg gtggacgcag tgcgcctggc ggccgggtgc gccgccgcaa acgccgagac
    10261 cctgggagcc ggggtcctgg acccccgccg cgccgaggag cttgccgaag acgtccagat
    10321 agagctccac aaccgcagct tccagagcgg gagggtgtag accggagggt aggcggacgg
    10381 cttttcaggg tcgtggagca tccacggaac gccccgtgag gcgtgctaga atgcgtgggt
    10441 tggagagcta ctcaggttat acggaggttc aagtttatgg cagttggcga ggtaactgac
    10501 gccacgttcg agggcgaagt cctacagtcg gagcagccgg taatagtgga tttctgggcg
    10561 ccttggtgcg tgccgtgccg ccggatctcc ccgatcctgg aggagatcag cgagagccgc
    10621 gatgacgtgc ggttcgtgaa gctcaacgtg gacgacaacc ccgccacggc gatgaactac
    10681 cagatttcga gcatcccgac catcactcgt ttcgagggtg ggcaggttac ccagcaggcg
    10741 gtcggagcgc tgccgaagaa ccagctctca gcccagctcg ggctgtaatt gcgctaagta
    10801 accgggacgg ttacaatctc ggcgtgaaat ttcccgaagc tcgggtgaac cgggtttgcg
    10861 gggtgttgtt cggaagatca gaggaggtac ggagatgaag ttcaagccac taggtgagcg
    10921 cgcgctggtc cagttggtcg agcgcgagga gaagacggct tccggaatcg tgctgccgga
    10981 tacggccaag gacaagccgc agacggcgga ggtcgtctcg gtgggcgagg gcaagctgga
    11041 cgacaagggt cagcgggtgc cgctcgaggt cagccagggc gacgtgatcg tcttcgccaa
    11101 gtactcgggg accgagatca agctcgacgg cgaggactac atgatcctcg acgccgacga
    11161 tatcctggga gtcgtggagg actagagtca gagactcaat tctcacggtc cctggagcgc
    11221 cgggcggaac ttctcgcccg gcgctcctct cttgaaccag ggcgggaccg gaggacgcac
    11281 ttggacgagg gaatgagacc gacggggaga ctcatgggga ggccggttcg gaccatgcgg
    11341 acgttacgca ggcgagatag agggcgcgag gtcgttgacc tccagacccg gcttcaggct
    11401 ctcggcttcg acctgggaaa ccgcggcata gacggggtct tcggtccccg caccgagctg
    11461 gcagtgaagg ctttccagca gtctatcggc atcctggccg acggactggt cgggccgatt
    11521 tcctggcggg agatcgtgga ggcgggctac cgtcccggcg ggcgcctgct ctacgtgcgc
    11581 cagcctccgt tccggggagc cgacgtggtg gagcttcagc ggatgctgaa cgacctcggg
    11641 ttcgaccccg gcgccgtcaa cggcctcttc gaccagcgca cggctcgggc cacgcacgac
    11701 ttccagaaga acgccgggct ccaggccgac ggcgtcgtgg acggggcggt cttcaaggtg
    11761 ctcgggacct atgccggaca gactctcggt acgcaccagc tcccggacaa gcacggcggc
    11821 tacttcccgg aggacctctt ctccggcacg atggtcgtgg acgccgccca cggcgggagg
    11881 gacaccggct acgtcgccaa gggcggtctc tccgaggcgg acctgaacct ggcggtggct
    11941 caggaactgg cccagatcct gccggccagc gaggtgttgc tcacccgcag cggcgacgat
    12001 gaagtgtctc ctgaagaccg ggcgttcctg gctaactcct cgggggcgaa gatggtgatc
    12061 tcgatccacc acaaccacca ccgcacccag acggcgaacg gcaccgccgc ctactacttc
    12121 gagcggctcg gctaccagtc gcaccggggc aacatggccg ccacctacat ccagcgcggc
    12181 gtcagcaagg ccctgggtac ctgcgatata ggggagttcg gacgctccta cgacatcctg
    12241 cgcgagacga acatccccgc cgtgatgctg gagcccctgc acctgacgaa ccccggcgag
    12301 ttgaagctcg cctcggaccc ttactacccg accacggtcg ccgaggccgt cgttggagcc
    12361 ctggagctgt acgccgggcg cgacgcggcg ttcatagccg tctagctcca agtggttccg
    12421 catggctgac agtcggcggc tgactgctat aatgtcctgc gcagtcggga cgtagcgcag
    12481 cctggtagcg cgcctcgttc gggacgagga ggtcggaggt tcaaatcctc tcgtcccgac
    12541 tgatcggggg aggtcccgga cctctcttta ctctctaatt cttcgtcccg atatccagac
    12601 cgaactgctc ggcggaactc aaacgctcga ctttcatcag gctctgcatg tggagcttgc
    12661 cgtttgcgta gctcccgccg tcctgcagcc ggatgtagtt ggtgtcgaac ttggcggtgc
    12721 gctcgatgct ttcgtatcct tgcctggctg ctagctctcc ctcgaactct acatccaggt
    12781 ctccctcgcc ggagacactg ccgcccggag tatccgcggt aaacgaacgc ggcctgacac
    12841 tgagctcgta gccctggatc caccccagct tccctcccga gcccatgctc tgcatgacgt
    12901 agcgcatgcg gtgcggtggg ccaccgaggt tggcgaacgg gatcggcatg tctcccgtcc
    12961 aggtgtcgtc ctccccgaca ttatcctgcg gcagcggggg gaagaggctc cagatcacct
    13021 cccgcaccga gatcagcgga acgggcgcct ctaatagagc cttgtctccc cgaacctccg
    13081 acacaacacc cggcgggctc atcttcacga cggccttctt gccggccagg tcctttaagt
    13141 ccaacgccac gggctctccc tcgccatctc taacgttcac ggcgaggtac agtaccttta
    13201 cctcgactga agactcagag atcggagtga cctggaaagc tcctgtcagg tccgaggcgc
    13261 cccggaggtc ggaggcggcg ccggagaagc tgctctcggc ggttacgctg actcggtaga
    13321 cggccggagc gtccagcgag cgcccggcgt agagcgtcga ggaggtgccg ccacacgaat
    13381 tcgagaaaaa gctggcgagc aggagcagtg tgagaggaag ggtgtttcta agcctcaaag
    13441 acctgcctct tcggcctctt ccagtagctg ggtccagtcg gtgtagaggc cgtcgaggat
    13501 gctctttagc tggcggtgtt cgaggacgac ttcgcgggag cgcttgccgt cggagtagag
    13561 ctccgatgtg gcgagctctt tctccaggcg cgcaacccgg cgttcggtcg cgtcgatctc
    13621 accttcgacc gcaactagac gggtcgcgag aatgctctcg gacttgctca tgccgggacg
    13681 taccctgcgg cgcggacggc gaacgccctc ctttacgtcc agccggcggt gagcgcggta
    13741 gtagtcgtag ccgccgaggt agttcgtgag cttcctgttg tcgagctcca cgatgcgggt
    13801 cgccagcttg cggaggaagt agcggtcgtg agagatgaag aagagagtgc cgcggtagcc
    13861 ggccagggcc tcttccagcg cctcgcgggc ggggatgtcc aggttgttcg tcggctcgtc
    13921 caggagaagg aggttcgcgc cgctcaccac tatctccgcg agggagagcc ggctcttctc
    13981 gccgccggag agcgaggaca ccttcttgaa cgcatcgtcg ccggagaaga ggaaagcgcc
    14041 caggaggtcc cgcgcctccg gcgcgtcgaa gcccgtcgcg tccatcacct cctgcaagac
    14101 ggtcttggat ccctccaggc gggcaagctg ctggtcctgg taggcgggct cgacgttgtt
    14161 gccgagccgg acgatgccgc cctggggctc gagttcgccc gtggcgaggc gcatgatggt
    14221 gctcttgccg gtgccgttgg gcccgagcag ggcgacccgt tctctgcgtt cgatcacgat
    14281 ctccaggtca tcgaggagcg gcccgtcggc gtcggggtga gcgtagcgga cgtcctccat
    14341 ctccagcacg acgcggcccg cgcgggaggt ctcgccgccg aggtccacgc gcatacgctt
    14401 cgagccgttc tttggaccct cgatcttgtc catccggtcc aggagcttct gcttactctt
    14461 cgcctgggtc gccttggtgt tcttggcccg gttcttctct atgaaacgct ctagctgcgc
    14521 ccggcgttca gcgttcgcct tcgccttgcg ggcgagctgc tcgtcgcggg ccttcttctc
    14581 ctccacgtat ttcgtgtagt ttcccgtgta gcggaccagt tttccatctt cgagctccac
    14641 gatggagtcc gaaatggcgt ccaggaaata gcggtcgtgc gagaccacga gcacggcgga
    14701 cttcgcgttc ttgatgaagg tctccagcca ctctatcgcg cgcaggtcga ggtggttcgt
    14761 cggctcgtcg aggaggacga gatccggctc ctcgaggagc aggcgggcga gcgccacccg
    14821 gctctgctcg ccgccggaga aggagccgac cgggcgtttc cagtcctcgg gggagaagcc
    14881 gaggccggag agggtagaag cggcgcgggc gcggtactcg taaccgccgt cccgctcgaa
    14941 ctcgctttgc aggcgaccgt agcgctcaag gagagcatcc gaggggttct cggagagctg
    15001 cgactccatc gctttcagtt ccttttcgcg ggagatcagg ggcgcgaagg ccgagatgat
    15061 ctcctcttcg atggaatgcg tgccccgctc tccagcgtag aggctctggg aggtcaggcc
    15121 gaccttcgcg ccgttcacgc gctccacgcc gccggaatct gcttcggagt tgccggccag
    15181 gatctcaagc agggtcgtct tgcccgcgcc gttgcgtccc accagaccga tcttctcccc
    15241 ggcctccacg gcgagcgacg ccccggcgag gaccttcagg tcgccgccgt ggtatttgac
    15301 gatatctctt gctataagga ccttcatgac gcagggatta taccggcaag acccgctgac
    15361 gccgaaggag cgcgaggaat tcgaggcgga cctcataacc ttctgcgacc gggagctgga
    15421 acgcctcgga gagatcaggg acttgcacgt actctacgcg ggcggcgtct cacctctgtg
    15481 gctcgaaggt ctgagccagc gcatcggacc caaaggcagc ctcaccgctc tggaggtcga
    15541 cggcgaacgc gccgcggcgg cgcgggagac gctccgggag atggatttat ccgctccggt
    15601 ggagatcgtg gagggggatg cctttgatcc gccgttcgag ccgcatagtc tcgacctcgt
    15661 gtattcggcg gggctctttc acgagttgga tgtgcgcgaa cgcccagccg ggggcgcgct
    15721 ggcggcgctg gcttccgtcg ttcggcccgg cggacgggtc gcgacgagcg acttcgtgga
    15781 ctcgacgccc gcggcccaga tcgaggacga ggccatcgaa cgcgagctgg cgcgggcggc
    15841 ctccggcgcg gttttgtatg gtataggtcc gccggagcgg ctcgtggagc ttcacgagca
    15901 ggtcctctcc ggcgtccgct ggatatcctc gccgccgttc gtaattcggc acctggacag
    15961 ggtggcgctc gcacaagacc ccttcgaggg ttgggacctg ccggatcaca cggtgcgcga
    16021 gcttcgccgc cgctacgacg tgctccgcgc gcgggttcgc cgtgaaggct acacccgtcc
    16081 cactaccctg tatgtcgagg ggatcgtgtc cggtaggtag cagacgaacg ctacgcgggc
    16141 gaagtgcgct accgctccaa gattatcgtg accgggccgt cgttcacgag cgccacgtcc
    16201 atcatcgcgc cgaaccggcc ggtcttcacc ggctcgacac ccgcagcgcg caaccgttcg
    16261 cagaagtagt cgaagagcgg ctcggccttc tccggcgcgg ccgccttgat gaagctgggc
    16321 cgtttgccct tgcgcgtatc cgccagcagg gtgaactgcg agacggccag gacttctccg
    16381 ccggtatcca gcgcgctgag gttcatcttg ccgtcttcgt cgttgaagat gcgaagcccg
    16441 gcgaccttct cggcgagcca gtcggcgtgc gcctctccgt caccctcggc caccccgacg
    16501 agcagcagca gccccggacc tatggaggag acttcctcat cctcgaccgt tacggaggcg
    16561 ctgcggaccc gctgcagtac gatccgcaat ggctttagag aggcttccgg cccgcgtagc
    16621 cggtctctat ctcgtgccag aactcgacgc gctcctctgg cgggtgccag cagaggaaga
    16681 cgacgcggcc gtcacgctcg tgcgggaagt ccagcaggcc catgtccagg tccttgacga
    16741 tcacaccaag ttcccggatg cgctctatcg cgaggtgcag gttgtacgcc tctacgccgt
    16801 actcggaccc catgcgcccg ccgccgttgg cgcccgcggc gcgcaggatg ggctccatgt
    16861 gcggcgcctt ctcgcgcacg gctgcgaggt ggagcgccag gtcgtcgagt aactctttca
    16921 gggttggtag cagggcgtta gcttcggcga cggtgaagag acgggaaaag gcgggctcca
    16981 ccgcgcggcc gtttctaacg gccctctgag agacggtcgg ccagcatcct ggggaggccg
    17041 gcggcccgga tcttggtctg cgccttcttg at
//
LOCUS       DASSMB010000085         7478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1285675, whole genome shotgun sequence.
ACCESSION   DASSMB010000085 DASSMB010000000
VERSION     DASSMB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7478
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285675"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..2061
                     /gene="aspS"
                     /locus_tag="VFJ72_05530"
     CDS             280..2061
                     /gene="aspS"
                     /locus_tag="VFJ72_05530"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET7478961.1"
                     /translation="MDGSKNPYRSHSAGELRGGDVGERVRLAGWVHRRRDHGGLIFID
                     LRDRWGITQVTFDPEREEVFASAEQLRPEWSVSVEGVVARRPEGNENADLPTGEIEVE
                     ATSMRVLNTSETPPFEIDRERPVDEMLRLKYRYLDLRRERMKENILFRHRVVKHIRDY
                     LAERDFVEIETPLLTASTPEGARDYLVPARLYPGQFYALPQSPQQFKQLLMVAGFERY
                     FQIARALRDEDQRGDRQPEHTQLDLEMSYTTQDEVLDLIEDLYIEIVEGLTEKKILAK
                     PFPRLTFAEAMDRFGSDKPDIRFGLELKDVSEIARSSEFKVFRGAVEGGGSVRGIAVG
                     GLGDLSRRGLDDLTAVAATGGARGLAHFKVADDGLSGPIAKFFPEEQQGALREALGAE
                     GGDYMFFVADKDPVVFESLNRLRLHFRDRLSLADPDVLGLCWVTDFPLFEWNEDEQRV
                     EPMHHMFTMPREEDIPLLDTDPLKVHGQLYDLVANGTELASGSIRIHLPELQQKVFST
                     IGIGPEEAERRFGAMLTAFKYGAPPHGGIAPGIDRTIMVLKDQPNIREVMAFPKTQAA
                     RDEMMDAPGPVTEDQLKELHISIRRKG"
     gene            2227..2856
                     /locus_tag="VFJ72_05535"
     CDS             2227..2856
                     /locus_tag="VFJ72_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7478962.1"
                     /translation="MKPAGLHPVHLTFDDGPDPLWTRRTLDALRDADARATFFVVAPL
                     ARQNPALVAEMIDGGHRVELHCAEHARHTERSRAEIVADTESALADLHAIGARPRLWR
                     PPWGVTAPWTTTIAERFGLELALWTIDPHDWRGDSPREMLAHIEPRLRPGAVVLLHDG
                     LGPGARRTGCGETAALIPALVHSIRARGLEPAPMTDPVAETPVLQRTPA"
     gene            2853..3989
                     /locus_tag="VFJ72_05540"
     CDS             2853..3989
                     /locus_tag="VFJ72_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478963.1"
                     /translation="MKTAGRIENGSVPLKEESLAGILRDIAAGADARDAEPSFPEGPF
                     RALAAADVLAIPVPDPVEATGRRASFTEEWRLLRAVARADGSVGRILDGHFNGVERLS
                     VCAPEPLRSRELDAVAAGELRLGVWGADPIPGEGDPARLMETATGLRVSGVKTFCSGS
                     TGLDRAVVLVRDAKGSPGPPLAAYVDLTEGMKMDRGWFGGSGMRSSESHRVEFHEASV
                     LAVFGAPGELLREPYFARDAIRTAVTWAGIADAALDAALEILEAKSSGREPDDLVSLA
                     AGRMLTAQGTIDRWLDYAAGRADEDYEASLAGFAVQLREAVANACREILDEAARAVGS
                     HPFAVSGTLDRARRDLELFLLQHRLEPAVARRGREEILARRREQ"
     gene            3986..4561
                     /locus_tag="VFJ72_05545"
     CDS             3986..4561
                     /locus_tag="VFJ72_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009312 - oligosaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009877 - nodulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET7478964.1"
                     /translation="MRQRLDRDYFESLYSESRDPWGFETSEYEKKKYERTLEALGGRH
                     FGRALEAGASIGVFTEMLAPYCDELLAVDLSEKALAVARERLSGQQHVRIEQRTLPEE
                     MPRGPFDLIVASEILYYWPREVVLRGLRRFEEELAPRGLLVAVHWRRETRTYPMQGDE
                     VHELLLENTRLTNVWSVSEPEYRLDLFEGLG"
     gene            4558..5730
                     /locus_tag="VFJ72_05550"
     CDS             4558..5730
                     /locus_tag="VFJ72_05550"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7478965.1"
                     /translation="MNRGKRLVIVGGGPAGLATARAYRESGGQAEVVLLAAESHPPYN
                     RPPLTKEYLRGEVDRGDLPIQQPGWFRENGIDLRLATRAQELDVDRRVVRTGSEKLAY
                     DACVIATGSEPIRLPVPGGDDPDIRTMRTIADSEILQKLVVPGSRAVVIGSGFIGCEA
                     AASLSMRGARVTLVSMEEAPQQERLGEEAGGRIRSWLEGYGVDLRLGTGVEEIGRRKD
                     GFTVGLEGEDEVEADVVLFGTGVSARIGLAEDAGIETKDGGIVCDSSMRTSEPGVFAV
                     GDIAFALNESAGRRLRVEHWGDALEHGRIAGTVLAGDEAAWSMAPGFWSTIGDKTLKY
                     WAWGDGWDDLSFEEDGDSFTVRYGKDGVLVGVLAHGADEDYEKGREEIERGVPLRG"
     gene            5727..6524
                     /locus_tag="VFJ72_05555"
     CDS             5727..6524
                     /locus_tag="VFJ72_05555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HET7478966.1"
                     /translation="MTLPPPHPALRACVVVPARNEEALIPACLEALAFQQHIVPEEYE
                     VLLVLDRCEDATETRAHEFAAAHPSLRLHFFNGPGLGAGHARRVGMEAACQRLHDLGR
                     SEGLIASTDADTVVSPDWLSAQLEAVSRGARAIGGRIHLAHDDSLPESVREWHAEYGR
                     TRYERLLAEPNFRGRVEHWQFSGASLALTAAVYREVGGLEPLPSLEDEHLERLLRRRD
                     IPIDYLLSVKVTTSPRLVGRAERGLSHDLALAAGRLASRSTGPSAPY"
     gene            complement(6710..>7478)
                     /locus_tag="VFJ72_05560"
     CDS             complement(6710..>7478)
                     /locus_tag="VFJ72_05560"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET7478967.1"
                     /translation="GPSPDKDPMAATPVEIPGGVVEALRNARSVTVLTGSGISAESGV
                     PTFRDAQSGLWARYDPMQLATPEAFVRDPQLVWRWYSWRRELIGAAAPNTGHRALVEL
                     ERRVPSFTLVTQNVDGLHQEAGSGLALELHGNIRRTKCSREDKTIESPGEEEPPRCPD
                     CGAYLRPDVVWFGEALPEGVMEGASRAARECDLFLSIGTSSLVYPAASLPYEALENGA
                     QVVEVNPEATPLTPHAAYSIRGSAGEVLPALVAAAYR"
BASE COUNT         1287 a         2492 c         2534 g         1165 t
ORIGIN      
        1 taccatcacc agacataacc agagtctggc gggccagcct ccaaccccgt agtctcggat
       61 aaactctccc ctgcaaaccg accgggagcc cgctacggaa ccgctgctcc ctctatgagc
      121 cagtgctaag gtatcacggt cgtaaaaacg gtgcaatcgg cctaccagag cccgcaacgc
      181 gcgccgcgtc cggcccggga gccaggaccg gcgatctacg acgtaaagac gcgacagagg
      241 ccacgtcagc agagaggaaa cagtactttg gagtcaaaaa tggacggatc gaagaacccc
      301 tacaggtccc actcggcggg cgaattgcgc ggcggcgacg tcggcgagcg cgtgcggctt
      361 gccgggtggg tgcatcggcg ccgcgaccac ggaggtctga tctttatcga cctccgggac
      421 cgctggggca tcacccaggt aactttcgat ccggagcgcg aggaggtctt cgcctcggcc
      481 gaacagttgc gtccggagtg gtccgtctct gtcgagggcg tggtcgcccg gcggccggag
      541 gggaacgaga acgcggacct cccgaccgga gagatcgagg tcgaggccac ctcgatgcgt
      601 gtcctgaaca cctccgagac gccgcccttc gagatcgacc gcgagcgccc cgtggacgag
      661 atgctccgcc tgaagtaccg ttacctggac ctgcgccggg aacggatgaa ggagaacatc
      721 ctcttccgcc accgcgtcgt caagcacatc cgcgactacc tggcggagcg cgacttcgtg
      781 gagatcgaga cgccgctact taccgcctct acgccggaag gcgcgcgaga ctacctggtc
      841 ccggcccgcc tctatcccgg acagttctac gcgcttcccc agtcgccgca gcagttcaag
      901 cagctcctga tggtggcggg gttcgagcgg tacttccaga tcgcgcgggc tctgagagac
      961 gaggatcagc gcggcgaccg ccagcccgag cacacccagc tcgacctgga gatgagttac
     1021 accacccagg acgaggtcct cgacctgatc gaagatctct acatcgagat cgtcgagggt
     1081 ctgacggaga agaagatcct ggcgaagccc ttcccccgtc tcaccttcgc cgaggcgatg
     1141 gaccgcttcg gctccgacaa gcccgacatc cggttcggcc tggagttgaa ggacgtctcg
     1201 gagatcgcac gctccagcga gttcaaggtg ttccggggag cggtcgaggg cggcggttcc
     1261 gtgcgcggca tcgccgtcgg cgggctcggc gacctctcgc ggcgcgggct ggatgacctc
     1321 accgccgtgg ccgcgaccgg cggggcgcgc ggcctcgccc acttcaaggt cgcggacgac
     1381 gggctctccg gccccatcgc caagttcttt ccggaggagc agcagggcgc gctgcgggaa
     1441 gctctgggag cagagggcgg cgactacatg ttcttcgtgg cggacaagga cccggtggtc
     1501 ttcgagagtt tgaaccggct gcggctccac ttccgcgacc gtctaagcct cgcggacccg
     1561 gacgtgctgg gtctgtgctg ggtcacggac ttcccgctct tcgagtggaa cgaggacgag
     1621 cagcgcgtcg agccgatgca ccacatgttc acgatgccgc gcgaggagga catcccgctg
     1681 ctcgacacgg acccgctgaa ggtccacggc cagctctatg acctggtggc aaacgggacg
     1741 gagttggcgt cggggagcat caggatccac ctgccggagc ttcagcagaa ggtattctcg
     1801 accatcggta tcgggccgga ggaggccgag cggcgcttcg gggcgatgct cacggcgttc
     1861 aaatacggcg cgccgccgca cggcgggatc gcgccgggta tagaccgcac gatcatggtc
     1921 ctcaaggacc agccgaacat ccgtgaggtg atggccttcc caaagactca ggccgcccgc
     1981 gacgagatga tggacgcacc cggccccgtc accgaagacc agctaaagga gcttcacatc
     2041 agcatccgcc gcaagggctg agaacgggac agttcacaaa actcgtaccg gagtgtgaaa
     2101 gatcgcccca tcgggcatga gggtcgctgt gctaacatcg tccagcattc caaagcaccg
     2161 accagatagg gagatcaggg tgagcagaga gacgtcgagc cgtacccggc gacgcggaga
     2221 agaccgttga agccagcggg cctccatccc gtgcacctga ccttcgacga cggcccggac
     2281 cccctctgga cccggcggac gctggacgcg cttcgagatg ccgatgcgcg cgccacgttc
     2341 ttcgtggtcg ccccgctggc acgccagaac ccggcgctag tcgccgagat gatagacggc
     2401 gggcaccggg tcgagttgca ctgcgccgag cacgcgcgcc acacggagcg ttcgcgggcg
     2461 gagatcgtag ccgacacgga gtccgcgctc gcagaccttc acgctatcgg cgctcggccc
     2521 cgcctctggc gtccgccctg gggcgtcacc gcgccctgga cgaccaccat cgccgagcgc
     2581 ttcgggctcg aactggcgct atggacaata gacccgcacg actggcgcgg agactcgccc
     2641 agagagatgc tggcgcacat cgagccccgg ctgcgccccg gcgcggtcgt cttgctgcac
     2701 gacggtctcg gtcccggcgc tcggcggacg ggctgcgggg agacggcggc cctgatcccc
     2761 gctctcgtac acagcatccg cgctcgcggc ctggagcccg ccccgatgac cgacccggta
     2821 gccgaaactc cggttcttca gaggacaccg gcatgaagac cgccggacgc atcgagaacg
     2881 gcagcgtccc tctcaaagaa gagagcctgg caggcatcct gcgcgacatc gccgccggag
     2941 ccgatgcccg cgacgccgaa ccctctttcc cggaaggccc gttcagggcg ctcgcggcgg
     3001 ccgacgtcct ggcgataccc gtcccggacc cggtcgaggc caccggccgg cgggcgtcct
     3061 tcacggaaga gtggcggctg ctacgcgccg tcgcccgcgc cgatggttcc gtcggacgca
     3121 tcctggacgg ccacttcaac ggcgtggagc gcctctcggt ctgcgccccg gagccgctgc
     3181 gctcgcggga gttggatgcg gtcgcggccg gggaactgcg gctcggagtc tggggggcgg
     3241 acccgatccc cggtgaagga gatcccgcaa ggctcatgga gacggcaact ggtctccgcg
     3301 tctccggcgt caagacgttc tgctccggct cgacgggcct ggaccgggca gtggtcctcg
     3361 tccgcgacgc caaaggttct ccgggtccgc ccctggcggc ctacgtggac ctgaccgagg
     3421 gcatgaagat ggaccggggc tggtttgggg gctctggcat gcgctcctcg gagagccacc
     3481 gcgtcgagtt ccacgaagcc tccgtactcg ccgtcttcgg cgcacccggc gaacttctgc
     3541 gcgagccgta cttcgcccgc gacgccatcc ggacggccgt cacctgggcg ggcatcgccg
     3601 acgccgcgct cgacgctgcg ctagagatcc tggaggccaa gtcctccggg cgcgagccgg
     3661 acgacctcgt atcgctggca gccgggagga tgctcacggc gcagggcacg atagaccgct
     3721 ggctggacta tgcggcgggc agggctgacg aggactacga agcctcgctc gctggcttcg
     3781 ccgtgcaatt gcgggaggcc gtagcgaatg cgtgccgcga gatactggat gaggcagccc
     3841 gcgcggtcgg ctctcacccg ttcgccgtct ccggcactct cgaccgcgcg cggcgagatc
     3901 tggagctgtt cctgctacag caccgcctcg aacccgccgt ggcgcgccgc ggacgcgagg
     3961 agatactggc gcggaggcgc gagcagtgag acagcgcctg gaccgcgact acttcgagag
     4021 tctctattcc gagtcccgcg acccctgggg attcgagacg agcgagtacg agaagaagaa
     4081 gtacgagcgc accctggaag ctctcggtgg gcggcatttc gggcgcgcgc tggaagcggg
     4141 agcttctatc ggtgtattca cggagatgct tgccccgtat tgtgacgaac ttctggcggt
     4201 ggacctctcg gagaaggcgc tggccgttgc tcgggagagg ctctccgggc aacagcacgt
     4261 ccggatcgag cagcgaactc tcccggagga gatgccacgg ggaccgttcg acctcatagt
     4321 cgcctccgag atcctgtatt actggccccg cgaagtcgtc ctgcgcggcc tgcggcgctt
     4381 cgaggaggag ctggcgcccc gcggactcct ggtcgcggtc cactggcgca gggagacccg
     4441 gacctacccg atgcagggag acgaggtaca cgagttgctg cttgagaaca cccgcctgac
     4501 caacgtgtgg agcgtctccg agcccgaata ccggctcgat ttgttcgagg gtctcgggtg
     4561 aaccgcggca agcggctggt gatcgtgggc ggcggcccgg ccggcctcgc gaccgccagg
     4621 gcttatcgtg aatccggcgg acaggcggag gtggttctgc tggcagcgga gagccatcca
     4681 ccatacaacc gtcccccact gaccaaggag tacctgcgcg gggaagtaga ccggggagac
     4741 ctgccaatcc agcagccagg ctggttcagg gagaacggga tagatttgcg tctcgccacg
     4801 cgcgcgcagg agctagatgt tgaccgccgc gtcgtgagga ccggctccga aaagctagcc
     4861 tacgatgcgt gcgtgatcgc caccggctcc gaaccgatac gcctcccggt ccccggtgga
     4921 gacgaccccg atattcgtac catgaggacc atcgctgact cggagatact tcagaagctc
     4981 gtggtgccag gcagccgggc ggtggtcata ggctccgggt ttataggctg cgaggcggcg
     5041 gcttcgctct ctatgcgcgg cgccagggta acgctcgtga gcatggaaga agcgccgcag
     5101 caggaacgtc tcggcgagga ggccggaggc cgcatccgct cctggctcga agggtacggc
     5161 gtggacctgc gtctcgggac cggggtcgag gaaataggtc ggcggaaaga cggtttcacg
     5221 gtaggcctcg aaggcgagga cgaagtagag gctgacgtcg tcctgttcgg aacgggggtg
     5281 agcgcgcgca tcggtctggc ggaggacgcg ggcatagaga ctaaggatgg cggcatcgtc
     5341 tgcgactcct cgatgcggac cagcgagcct ggagtcttcg ccgtcgggga tatagctttc
     5401 gcgctcaacg agtctgccgg acggcggctg cgagtagagc attggggcga cgctctggag
     5461 cacggccgga tcgccggtac ggtccttgcc ggtgacgaag cggcgtggag catggctccg
     5521 ggcttctggt ccaccatcgg ggataagacg ctcaaatact gggcctgggg cgacggctgg
     5581 gacgacctga gcttcgagga ggacggcgac tcattcaccg tccgttacgg caaggacggt
     5641 gtgctggttg gggtgctggc ccacggcgcg gacgaggact acgagaaggg acgcgaggag
     5701 atcgaacgcg gagtcccgct gcgcggatga cgctcccccc tccccatccc gccctgcggg
     5761 cctgcgtcgt ggtccctgcg aggaacgagg aagccctgat ccccgcctgc ctcgaagccc
     5821 tcgcgttcca gcaacacatc gtgccggaag agtacgaggt gctgctcgtc ctcgaccgct
     5881 gcgaggacgc gaccgagaca cgcgcccacg agttcgccgc ggcgcaccct tccctgcggc
     5941 tgcacttctt taacggcccc ggcctgggag ccggtcacgc caggcgggtc ggcatggaag
     6001 ccgcgtgcca gcgtctgcac gacctcggca gaagcgaagg tttgatcgcc tctaccgatg
     6061 cagataccgt cgtgagcccg gactggctct ccgcccaact ggaggccgtc tcccgcggcg
     6121 cccgagccat cggcggaagg atccacctgg cccacgacga ttcgcttccg gagagcgtcc
     6181 gcgaatggca cgccgagtac ggccgtactc gctacgaacg actgctagcc gagccaaact
     6241 tccgcggcag ggtcgaacac tggcagttct ccggcgcctc gctggcgctg accgccgcgg
     6301 tctaccggga agtcggcggc ctggagcccc tgccatccct tgaagacgaa catctcgaac
     6361 ggctcctgcg gcggcgcgac atccctatag actacctgct ctcggtaaag gtcacgacgt
     6421 cgccccgtct cgtgggccgc gccgagcggg gcctctctca tgacctcgcc ctggcggccg
     6481 gcaggctcgc cagccgctcg acgggaccgt ccgcccccta ctgagccttc tcctcccggt
     6541 gctccaggat gtcctcgcac aactctttcg ggaacagtag ttcgttgtag atgttgctgg
     6601 agtcggggtc gccggggctc gggcagatct ggaactgctg ttactgccac ccgagtatgc
     6661 gctcgcgcgg cggcaggttg tagtccttct ccggctaact cgcggcggat cagcggtagg
     6721 cggccgcgac gagagcgggc agcacctccc cggcgcttcc acggatcgag tacgcagcgt
     6781 gcggagtgag cggggttgcc tccgggttga cctccacgac ctgcgcgccg ttttccagcg
     6841 cctcatacgg cagcgaggca gccgggtaca cgaggctcga agtgccgatg gagaggaaga
     6901 ggtcgcactc ccgcgccgcc cgcgaggcac cctccatcac tccttccggc aaagcctctc
     6961 caaaccatac gacatccggg cgcaggtacg cgccgcagtc cgggcagcgc ggcggttcct
     7021 cctcgccggg tgactcgatg gtcttgtcct cccgggaaca tttcgtgcgg cggatgttgc
     7081 cgtggagttc cagagccaag ccgctgcccg cctcttgatg caacccgtcg acgttctgcg
     7141 tgacgagtgt gaatgatggg acgcgacgct ccaattcgac tagggcacgg tgacccgtgt
     7201 tgggcgccgc cgcaccgata agctcgcgcc gccacgagta ccaccgccat acaagttgcg
     7261 ggtcacgaac gaacgcctcc ggcgttgcaa gctgcatggg atcgtaacgc gcccagagcc
     7321 cggactgggc gtcccggaag gtcggcaccc cgctctcggc cgagattccc gacccagtga
     7381 gtactgttac gcttcgggcg ttgcgcaggg cctcgacaac gccaccgggt atctctaccg
     7441 gcgtggcggc catcgggtcc ttatccggcg acggcccg
//
LOCUS       DASSMB010000086         4038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_904712, whole genome shotgun sequence.
ACCESSION   DASSMB010000086 DASSMB010000000
VERSION     DASSMB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904712"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1587
                     /gene="menE"
                     /locus_tag="VFJ72_05565"
     CDS             136..1587
                     /gene="menE"
                     /locus_tag="VFJ72_05565"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR01923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HET7478968.1"
                     /translation="MSPRYDALQAPCPLRAAALAAPDAPAILGSAGGISYAELDRRVS
                     GTAENLTENGCAPGMRVALYLEKDERYLVLLLALLRAGCVVCPVSTRVPAEGVVALLG
                     TATCSALISAGDFHPADGASLTVLRPDSFLQEASEAAVLEPLDIPLNRPATIIFTSGS
                     TGTPKAALHTFGNHYYSALGSGANIPLASDDRWLHSLPLYHVGGLSIVFRCLLSGAAM
                     AMPEAGIPLGEAIARSGATHVSLVATQLRRLLAQNHSSETLKAVLMGGGPVPDTLVDE
                     ALDRGLPVHTSYGLTEMASQVTTTPPDASRAARHTSGRVLPHREVSVAEDGEILVRGD
                     TLFAGYVEGDVVDPRLDAEGWFHTKDLGELDADGYLRVTGRRDNQFVSGGENVQPEEI
                     EAALCKVRGVEEAVVVPVPDEEFGERPVAFVRAPTGLPEDLAKALEPVLPRFKIPAVF
                     HEWPEFSGPEGMKPDRAAFRKLAQRGKGTGAVP"
     gene            complement(1622..2956)
                     /locus_tag="VFJ72_05570"
     CDS             complement(1622..2956)
                     /locus_tag="VFJ72_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7478969.1"
                     /translation="MALDYTYCIQLPHRAGSLAKVAGKIAEGNGLIGDVTTIRVGRES
                     SIREITLEVEDQAHGESIVGLIGELPGVEVLWARDRALLRHEGGKLKIESTRPVRTVQ
                     DMRDVYTPGVARACQAIAADPSLASNLTMIGRSVAICTNGTRVLGLGDIGPVASMPVM
                     EGKAVFYHQLAGVSAMPILIDTKDVDEFVETVVRIAPTFGGIHLEDISAPECFEIEAR
                     LIEALPQPVMHDDVHGTAVVTLAAAMVACRRAGITLEDEVVGQIGLGAAGFGIASLMV
                     DGKAKRVIASDPNPLSHERARDKGIEITDMQTVMREADVVVATTGRPDLISPEMVREG
                     QVILALTNPYPEIDPEVAIAAGAAFAADGTSVNNVLGYPGLFRGALLAGAEMINVPMK
                     LAAAEAIAGLATESELVPDVLNPDVHERVATAVRDAAIKSGVARLDQVPSGL"
     gene            3088..>4038
                     /locus_tag="VFJ72_05575"
     CDS             3088..>4038
                     /locus_tag="VFJ72_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7478970.1"
                     /translation="MQRTFDVSAVREQFPALRRTHNGRPVAYFDGPGGSQVAGPAIEA
                     MSRYMERGGANLHGAFPTSIETEEIIADTRHAMAAFLGAEPDEIAFGANMTTLTFAVS
                     RALARGWDERSEIVLTELDHRANVDPWLLAADEKGAKTRWLPVDSETLTLDLSNLETT
                     INERTRLVAIGLASNAVGTVNPVAKIAERAREVGALVAVDAVHAAPHIPIDRDEIGAD
                     ILTCSAYKFFGPHVGIIAIRRDLLEALPVYRLEPAPDYIPDMLETGTQNHEGIAGVGG
                     ALDFISSLGTGDSRRERLVSAMRMIEDHEDALAARFRSALG"
BASE COUNT          655 a         1412 c         1284 g          687 t
ORIGIN      
        1 tatatgcagc gagcatatag cgtcgagtct ccattgtgaa cagcgccact ctgacaggca
       61 ttgattgctt cgacttgccg gtaattacgt gcagtagcgg tgcaggagcg tgtattcgtc
      121 ggataatatc gtcctgtgag cccgagatac gatgcgctgc aagcaccctg ccctctgaga
      181 gcggcggctc tcgccgcgcc ggatgctccg gccatcctgg ggtctgccgg tgggatctcc
      241 tacgcggaac tcgaccgccg ggtctccggg actgcggaaa acctcacgga gaatggatgc
      301 gctccgggga tgcgcgtggc gctctacctc gaaaaagacg agcggtatct ggtcctgcta
      361 ctggctctgc tcagggccgg ttgcgtggtg tgtcccgtga gcacccgtgt tccggctgaa
      421 ggcgtcgtgg ctctgctagg aaccgctacc tgctccgccc tgatctccgc tggagacttc
      481 cacccggctg acggcgcaag cctgaccgtc ctgcgcccgg actcgttctt gcaagaggcc
      541 tcggaagccg ctgtcttgga gcctctggat attccgctga accgtcccgc caccataatc
      601 tttacctccg gcagcacggg gacgccgaag gccgctctgc acaccttcgg caaccattat
      661 tacagcgcgc tcggctccgg cgcgaacatc ccgctcgcgt ccgacgaccg ctggctgcac
      721 tcgctgccgc tctaccacgt cggcggactc tctatcgtct tccggtgtct gctctcgggc
      781 gcggcgatgg cgatgccgga ggccggtatt ccactaggag aagctatcgc ccggagcggt
      841 gcgacccacg tgtcgctggt cgccacccag ctccggcgat tgctcgccca gaaccactcg
      901 tccgagaccc tcaaggccgt cctgatggga ggcggtccag tcccggatac gctggtggac
      961 gaggcgctcg accgcgggtt gccggtccat acgagttacg gcctgaccga gatggcctcg
     1021 caggtgacca cgacgccacc agacgcctcg cgggcggcgc ggcatacttc gggacgggtg
     1081 ttgccgcacc gcgaggtctc cgtggcggag gacggggaga tactggtgcg cggggatacg
     1141 ctcttcgccg ggtacgtcga gggtgatgta gtggacccga ggctcgacgc cgaaggctgg
     1201 ttccatacga aggacctcgg cgagctggac gcggacggct acctgcgcgt cacggggcgc
     1261 cgggacaacc agttcgtctc cggcggcgag aacgtgcagc ccgaggagat cgaggcggcg
     1321 ctctgcaagg tgcggggcgt cgaggaggcc gtggtcgtcc cggtcccgga tgaggagttc
     1381 ggggagaggc ccgtggcctt cgtgcgcgct cccaccggat tgccggagga cctggcgaaa
     1441 gccctggagc ccgtgctgcc gcgcttcaag ataccggctg tcttccatga gtggcccgag
     1501 ttctccgggc cggaagggat gaagccggat cgcgccgcct tccggaagct cgcgcagcgt
     1561 gggaaaggga ccggcgcagt cccttgaggg cctggccgcg cggactttac tactgacccg
     1621 gctacaggcc ggacggcacc tgatccaggc gggcgacgcc gctctttatg gccgcgtctc
     1681 tgacggcggt cgctacccgt tcgtggacgt ccgggttcag tacgtcgggg acgagctcgg
     1741 actcggtggc gagtcccgct atcgcttccg cggcggccag cttcatcggg acgttgatca
     1801 tctcggcccc ggcgagcagg gctccgcgga agagacccgg atagcccagc acgttgttca
     1861 cgctcgtccc gtcggcggcg aaagccgcgc cggccgctat agcaacttcg gggtcaatct
     1921 cggggtaggg gttggtgagg gccaggatca cctgcccttc tcgtaccatc tccggtgaga
     1981 taagatccgg tcgtccggtc gtggcgacga ccacgtcagc ctcgcgcatg acggtctgca
     2041 tgtccgtgat ctcgatgcct ttatcgcggg cgcgctcgtg ggagagcggg ttcgggtccg
     2101 aggcgatcac gcgcttcgcc ttaccgtcca ccatgagaga ggcgatcccg aaccccgccg
     2161 ccccgagccc gatctggccg accacctcat cctccagcgt gatgcccgcc ctgcggcagg
     2221 ccaccatcgc cgccgccagc gtgacgacgg cggtgccatg cacgtcgtcg tgcatcacgg
     2281 gctgcggcag ggcctcgatg agccgcgcct cgatctcgaa gcactccggc gcggagatgt
     2341 cctccaggtg tatgccgccg aacgtcggcg cgatgcggac caccgtctct acgaactcgt
     2401 caacgtcttt ggtgtctatg aggatcggca tcgccgatac gcccgcgagc tgatggtaga
     2461 agacggcctt cccctccatg accggcatcg aggccacagg gcctatatct cccaggccca
     2521 gcacccgcgt gccgttcgtg cagatggcga cgctgcgccc gatcatggtc aggttgctcg
     2581 cgagcgaggg gtcggcggcg atggcctggc acgcccgcgc cacgcccgga gtgtacacgt
     2641 cgcgcatgtc ctgcacggtc cttaccggcc gcgtggactc tatcttcagc ttcccgccct
     2701 cgtgacgcag gagcgcccgg tcgcgcgccc atagcacctc gacgcccgga agctccccaa
     2761 tcagcccgac gatactctcg ccgtgggcct ggtcctcgac ctccagcgtg atctcccgta
     2821 tagaggactc gcgccccacg cggatggtcg tcacgtcccc gatcaaaccg ttcccctcgg
     2881 ctatcttgcc cgcgaccttg gcgagcgatc ccgcccggtg cggcaactgg atgcaatagg
     2941 tgtagtcgag cgccaaaacg acctcctcta atacctgaca cgcaaggaca cattatcatc
     3001 accggagtct ccaggcgcgg gttcccaccg gcccgctgat gtagactcgc tcatcgagag
     3061 agacttacga gatgccagga ggactccatg cagcgcacct tcgacgtatc cgccgtccgc
     3121 gagcagttcc cggctctgcg gcggacccat aacggtcggc cggtcgcgta cttcgacggg
     3181 cctggcggct cccaggtcgc gggtcccgcc atcgaggcga tgagtcgcta catggagcgg
     3241 ggtggcgcga acctgcacgg cgccttcccg acgagcatcg agaccgagga gatcatcgcg
     3301 gacacccgcc acgctatggc cgccttcctc ggcgccgaac ccgacgagat agccttcggc
     3361 gccaacatga ccacactcac cttcgccgtc tcccgcgccc tcgcccgcgg ctgggatgag
     3421 agatccgaga tcgtcctcac cgagctcgac caccgcgcca acgttgaccc ctggctcctg
     3481 gccgccgacg agaagggcgc gaagacccgc tggctccccg tggactccga gaccctcacc
     3541 ctggacctct cgaacctcga aacaaccata aacgagcgta cccgtctcgt cgccatcggc
     3601 ctcgcttcca acgccgtcgg cacggtaaac ccggtggcga agatagcgga gcgcgcccgc
     3661 gaggtcggcg ccctggtggc tgtggacgcc gtccacgccg ccccgcacat ccccatagac
     3721 cgcgatgaga taggcgcgga catcctcacc tgctccgcct acaagttctt cggtccccac
     3781 gtcggtatca tcgccataag gcgcgaccta ctcgaagcgt tgcccgtcta ccgcctcgaa
     3841 cccgccccgg actacatccc cgatatgctg gagaccggca cccagaacca cgagggcatc
     3901 gccggcgtcg gaggcgctct cgacttcata tcctctctcg gaaccggcga ctcgcggcgc
     3961 gagcggctcg tgagcgccat gcggatgatc gaggaccacg aagacgccct cgccgcccgg
     4021 ttccgctcgg cgctcggc
//
LOCUS       DASSMB010000087        14456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_19174, whole genome shotgun sequence.
ACCESSION   DASSMB010000087 DASSMB010000000
VERSION     DASSMB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19174"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VFJ72_05580"
     CDS             complement(<1..494)
                     /locus_tag="VFJ72_05580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET7478971.1"
                     /translation="MRFGISTFVTDEGIAPGTLARAIEERGFDSLFIAEHTHIPLSRK
                     SPYPGGGELPLKYYRTLDPFITLTAAAAATERLLLGTGVALIVERDPIITAKEVASLD
                     LVSGGRMVFGVGVGWNREEMENHGTDPRTRGRLLDERLRAMIEIWTKDEPEFHGEYVD
                     FDPIA"
     gene            complement(759..1616)
                     /locus_tag="VFJ72_05585"
     CDS             complement(759..1616)
                     /locus_tag="VFJ72_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478972.1"
                     /translation="MGHVAIAVVLSDGDEEDRCRAAAEVVDVYSRVREYSPEELEGDD
                     APTYAEVLTALRLKSRGLEAALPSRRVLAHLLAGLDYYLFALPDLGYVYDMPRLTDEE
                     RAAEHGLRHDLLLKLYDELEPAFDVSLSWPQQGYEVDENGVLIKDDVNRMEAIGFDCG
                     CDPPRLTTGGAWRSSLSGSRSVCCSGARPSPASPRDAGRSRCRRRSRAGDGAVGRASA
                     HRARYRWTVGPRKLAGPSEVQRTRIASTYSSSRAPRLWKGIPRASNSWGSHPAGDALV
                     NTSRVRGTE"
     gene            2027..2398
                     /locus_tag="VFJ72_05590"
     CDS             2027..2398
                     /locus_tag="VFJ72_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478973.1"
                     /translation="MEAQVLEQAHRQAVVTPVGDVVSFLQDLLGRRLVTYVAGVKDAK
                     TVSRWASGEVGSIRQESEERIRTAYEVAQLLVQFDSPRIVKAWFIGLNPQLDDVSPAE
                     TIREGKLKEAKAAARAFVAGG"
     gene            2430..2825
                     /locus_tag="VFJ72_05595"
     CDS             2430..2825
                     /locus_tag="VFJ72_05595"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HET7478974.1"
                     /translation="MVYRVARGLDAFAPPDWAYALPDGTFGNRFDDPAAADKGVPEEE
                     RYRAVYCATERAGAFGETIARFRPSMELLAGLEEVEDDEPLDPELRGGVVPEEWRLSR
                     RARAPPGSPPACCSPISPLQRPCVSCERT"
     gene            2831..2995
                     /locus_tag="VFJ72_05600"
     CDS             2831..2995
                     /locus_tag="VFJ72_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478975.1"
                     /translation="MAEKLGLEDLDLSAVTGPHRRLTQEAARYVYDLEDSSGSLAFAG
                     IRYISRLNPS"
     gene            3201..4031
                     /locus_tag="VFJ72_05605"
     CDS             3201..4031
                     /locus_tag="VFJ72_05605"
                     /inference="COORDINATES: protein motif:HMM:NF033520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HET7478976.1"
                     /translation="MEDTITTTYYLCDEFLKAVGHRDDPQARLSTAEVMTVPLVACTF
                     FSGNLEASRAFLDGYGYIEKTISKSRFNRRLHAIDSCLWEGLFALLAEVFKHEHSERT
                     YVVHSLPVAVCDNIRIRRCRLYPPEEHGGAFRGYIASKRRYFYGLRVHLVVTGAGEPV
                     EFSLAAGAQADVSVFKNLGLDLPVGSTILADKAYTDYDYEDLLEEVGLHLKAQRKKNS
                     KRPMPMWEEFLGKSIRQYIETVFSRLSALFARKIHAVTPRGFELKIACFLLAFSIQCL
                     "
     gene            complement(4104..4856)
                     /locus_tag="VFJ72_05610"
     CDS             complement(4104..4856)
                     /locus_tag="VFJ72_05610"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7478977.1"
                     /translation="MTEAGGIEVGDNGSLGPLRRAETLSDRATDLLRERILAGDFGLG
                     ERLVEAKIAKRLEISRGPVREALRQLRAEGLVREEPRRGSFVIDLTIEDVREIYDLRA
                     AIEGRAARLIITSKDLAALDELREILHQLERAADEGDRTLFTKLDLAFHERLCLLSGN
                     GRLHRVFVSYAAVLGLIFRLEVDKIYTSRSSLEDLYGDHEKLFRAIESLDARRAEEAC
                     DEHLDRAREQLIKVFGGSLAKSERAADARPGA"
     gene            4986..6407
                     /locus_tag="VFJ72_05615"
     CDS             4986..6407
                     /locus_tag="VFJ72_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7478978.1"
                     /translation="MTLSPGDNPEARSRLEELGYSQELKRRLKMWHVVGLAMADVSPT
                     MAVLFLTAGVFFVGGTFALGANLILAVVVVLIALCLAELAAMYPIAGGMYSLVRYVLP
                     GPITWITMFNYLLQGIVIPASIALGVATFLQDLVPALDALPVPLLALISLAVATVIAL
                     MTIEVGAWLTFIMVVVELVVLGLVTVAAFANPNQNLAEVVFHPVVLHDGSLAAVGFGV
                     MLATLAPAFNVINGYDATLGFSEEMIGGERSIPKAVVSAAVLASLFIIVPLIAAVVAA
                     PDLKAFFNADAPIVYSVEQAMGRGAGMIVDFGASIALFNAMLSLLMYFGRGVYTTGRD
                     GIWPPPVSRVLARVNRFGVPAAGVLALAVPAAILIFLTALDFLIIFAGTMIAAVYFCI
                     GLAALWSRISQRDEPRPYRMPLWPLTPIVVVVFTGVALVLQGSEYLIAELVLAAVAVA
                     LWAVSKTWSGAQRSTETPAEAER"
     gene            6404..7144
                     /locus_tag="VFJ72_05620"
     CDS             6404..7144
                     /locus_tag="VFJ72_05620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET7478979.1"
                     /translation="MKDRVVAITGAGRGIGLATARRFAEKGCRLALNHWRTGTKALQE
                     VAGLARENGGDAFLVEADIGRPEEAARFVRESEARFGGIDVLINNAGILRSTPTHEET
                     QANWDEVISVNLGGTWSCLRAALPGMLERGSGRIINVSSELGIIGYPTYAAYCASKGG
                     IIALTKAVAKEVAPRGVLVNSVAPGPVETDMLIHDTIEYNDETREQIPLRRFGRPEEI
                     AAAIEFLAGEGGSFMVGQILSPNGGTAI"
     gene            7171..8082
                     /locus_tag="VFJ72_05625"
     CDS             7171..8082
                     /locus_tag="VFJ72_05625"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7478980.1"
                     /translation="MTTTMVTGGLGYVGRHLVARLAERGERVVSYNRDYAEGGEENVT
                     YVQGELYDLPRFVDTIGRYEVGRVIHTAAMSHPDLSIDLPITTFTANVDGTVHVFEAA
                     RMSGVRRIVNFSSETAYGHVEAEPVTEDAPLHPTTPYGVTKVATELLGQVYSDLYGLD
                     VISLRISEVYGPGNRMPQFLREMINSALQGEPYRLEKGGDHRFQFIRVEDVARAAILA
                     ADAERTSRRVYNITGGSQVTLAQAAEIVRGEIREADVEIGPGHLHLDRQGPYDITAAG
                     RDLGYRPEWSVECGIHDYVDWLRDHPY"
     gene            8108..8875
                     /locus_tag="VFJ72_05630"
     CDS             8108..8875
                     /locus_tag="VFJ72_05630"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047789 - creatininase activity [Evidence
                     IEA];
                     GO_process: GO:0046449 - creatinine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HET7478981.1"
                     /translation="MEQRTRLMAEMTWPEVEAAIRRGAGVVLPVGSTEQHGHHLPLAT
                     DAILPTELALAVAGPLDLLVAPAVTYGYRSRPLSGGGQGFVGTTSLRATTLIALVEDV
                     LRELIRQGFKRIVLLNWHMENQNFVYESAYLALEQSRESQARVLVAELAFTDLSSETM
                     RALFPEGFPGWDVEHASILETSLMLHLHTEMVLFDRAVDDAAKRHPWYDVLPVPADFV
                     PRSGTLWKATQASAEKGRMAWREIVEQLREAISAELG"
     gene            8973..9242
                     /locus_tag="VFJ72_05635"
     CDS             8973..9242
                     /locus_tag="VFJ72_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7478982.1"
                     /translation="MWSCIRTEEERLSNSRRNHTREFKGPAQRRHECLVERWKEIRKL
                     HLAGARVKDIAEWVGTSQSTVYRYRELAEPPPRPSYRRRASVLDP"
     gene            9391..9747
                     /locus_tag="VFJ72_05640"
     CDS             9391..9747
                     /locus_tag="VFJ72_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478983.1"
                     /translation="MPHAPRAKRGYIAGASPTAKNVAALFMRREEMLNEEQKAYIKRL
                     CASDAALADARRLTQRFATMVSNLEGENLDGWLEKAAGCEATVMKRFASGLEKDLERC
                     ARVFPRSGRTGRWRAS"
     gene            complement(9946..11889)
                     /locus_tag="VFJ72_05645"
     CDS             complement(9946..11889)
                     /locus_tag="VFJ72_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478984.1"
                     /translation="MTIIRFKISRTRRSMTVFAAGLLVAIVLGLLLMTGPARAVPLPC
                     DTCDPGGGTNTAPTVAANNATVTINESQTANNTGTYSDADGDPVTLSVTSGPGNVVPD
                     AAGPGTWAWSYGTSDGPSQNATVTITASDGTDSSTVSFSLAVNNVAPNGSFRYPTSVD
                     EGGPIGLSLTNPTDPSGVDTAAGFSYAFSCRDSLESGAGVFGNTFLFWTSHYGTLGSG
                     NSANCPTDDNGSTTVRARIQDKDGGFTDSQPTTPSGCSLFSCPAGTTNPAYTGSVTIN
                     NVAPTATFNVSTTTVDEGSGGFTLSLTSPLDPSTADRNAGFSYAFDCGSGYGTFGSSS
                     SASCPAGDGPATLAVGGKIKDKDGGVSEYANSVGVSNVAPQGNFNAPSSVNPDTQIPL
                     SFTEPSDPSAADTNSGFSYAFDCGLGQGYTTSQSSSQAFCTAGDGNSQTVRGKIIDKD
                     GGTTEYTRDVTINDITAPETTLDATGPSSITNSTSASFGFSSEANATFQCSLDGAALS
                     ACTSPKSYTSLPNGSHTFRVAATDAAGNTDATPAARSWTVDTIKPTITRMSPKPASTI
                     TDRTPTIKATIKDNVTNLQKSNIKLYVNGARISATKYSYSASTDVLTYNSPKLARGKK
                     TVKVVATDAAGNVGARYWYFTIK"
     gene            12850..>14456
                     /locus_tag="VFJ72_05650"
     CDS             12850..>14456
                     /locus_tag="VFJ72_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7478985.1"
                     /translation="MSARTQTRSLVLAFIALGIAGALLAVIAVAPPTLARSARPNEGL
                     RVLSPGERPSGSRTPGSLSALQKRALIKGYLVPDQQGYELAKAAADRKAAAGTGQEAP
                     FAPASAAPTSFRSWQGARDAGGAPSDSTGAIGPGRYVELVNSRYAIYNRTSNVPINQG
                     SLNDLVGAAALESVFDPQIIWDPTTNRFYYAADDVLDSANNFLAFGFSKDSTPDAGPS
                     DWCQYFVAYGADFPDYPKLGDTRDFVMIGSNVFPAAGGFHSSLDWIRKPPAGAGCPNL
                     SSGVKTDLRNSDGTQAITPVPANQTDTSPTGYVVAEPASVPNGAGPAGFLSLFTVSSN
                     TDGTLALSAPRNVTVPGYDLPANAPQRGTTRRLDTSDSRNTQAVSAIDPSRGTGGLTA
                     LWTQHTVFGGAGAQVRWYEINPASATLFQRGTVSGSSANGVFDFNGAISPDRKVLGTT
                     RKFGSSMILGYNSSSATQFPSVQMISKVGSNAASAGTLVKASAASLQNFDCSDPNFPG
                     VCRWGDYSAATPDPGSSTTTTHGQVWLT"
BASE COUNT         2594 a         4624 c         4606 g         2632 t
ORIGIN      
        1 gcgatcgggt cgaagtccac gtactctccg tggaactccg gctcgtcttt ggtccagatc
       61 tctatcatgg cccgtagacg ctcgtctagc agcctcccgc gggttcgcgg gtcggtgccg
      121 tggttctcca tctcctcccg gttccaaccc acaccgacgc cgaagaccat ccgtcccccg
      181 gatacgaggt cgaggctagc gacctccttg gcggtgatga tcgggtcccg ctcgacgatc
      241 agggctaccc cggttccgag cagcagccgt tcggtcgctg cggccgcggc ggttagcgtg
      301 ataaagggat cgagggtccg gtagtacttt agcggcagct ctccaccacc gggataggga
      361 gacttccggc tgagcgggat gtgggtgtgc tcggcgatga acaaagagtc gaagccgcgc
      421 tcctcgatcg cccgggctag tgtccccggc gcgatcccct cgtcggtcac gaacgtcgag
      481 ataccgaacc tcatgccccc attgaaccat actgtcaggg caggcggagc gggctacccg
      541 ccggactccg taatgctaaa gacgtggtac cgccgttcgc ggcagcgcag ccgctcgccg
      601 cgttcatgat tgtttgccgg gaccatgact ttgccagccc cgctgtgtac ggcgagttac
      661 atgctactcg cccgaagggt ccgcaggtct cccgtgagct gcaccgaacg gcactcatca
      721 gatacggatc ggtactcgcc agagcgacca cgggatgatc actctgtgcc acggacccga
      781 gaagtattga caagagcgtc accagccggg tggctccccc aggagttcga agccctcggt
      841 atacccttcc acaaacgcgg agccctgctg gacgagtacg tcgaggcgat gcgcgtcctc
      901 tggacctcgg acggaccggc tagtttccgc ggtcccacag tccaccgata acgtgctctg
      961 tgagccgaag cccgtccaac ggccccatcc cccgctctgg atcggcggcg gcagcgagac
     1021 cgccctgcgt cgcgcggcga agccgggctc ggcctggcac ccgagcagca aacactccga
     1081 gaccctgaca gagaagatcg ccatgctccg ccggttgtca gccgtggagg gtcacagccg
     1141 cagtcgaagc cgatggcctc catgcggttc acgtcgtcct ttatcagcac gccgttctca
     1201 tccacctcgt agccctgctg cggccaggag agggagacgt cgaaggccgg ctccaactcg
     1261 tcgtagagct tgaggagcag gtcgtgtcta aggccgtgct cggcggcgcg ctcctcatcc
     1321 gtcaggcgcg gcatgtcgta gacgtacccg agatccggca acgcgaataa gtagtagtcg
     1381 aggcccgcca gcaagtgagc gagcaccctg cgagacggaa gggccgcttc aagtcccctg
     1441 ctcttgagcc tgagcgcggt caggacctcc gcgtaggtgg gagcgtcgtc gccctccagt
     1501 tcctcgggcg agtactcgcg tacccgggag tagacgtcca ctacctcggc cgccgcacgg
     1561 caccggtcct cctcgtctcc gtcagacagc accaccgcga tggccacgtg tcccatgagc
     1621 gcgtacttga aggaggcgca ggctgcgctc ctcgtccgtc acatcgggcg gccgtgtatc
     1681 cttcatccgg cttctctgtt cctcactgca gtcttcctct cctcgagccg tacactagat
     1741 ctggatagat cgtcttcgta taccgcgcgc agcagcttat tttgcacata taccgggctc
     1801 ccgcggagca aacctggcta cgtcgtccgt cgcggaggcc ctggagcgcg gtctggcacg
     1861 ccgcaagcgg ctactggaga caccggaggg aaaagggcgt aggttatcgt gaagaggaca
     1921 atgtctgagt tgaggcagta gtaggcgctg gagaccagcg ggtgtagaat gaaaggacat
     1981 tagtagggca ttaaaagggc acgagtgtcc gcgaaagggg tgcgtgatgg aggcacaggt
     2041 cctggagcag gcccaccgtc aggcggtggt gacgccggtc ggtgacgtgg tctctttctt
     2101 gcaggacctc ctggggcggc gcctggtcac ctacgtggcg ggggtaaagg acgcgaagac
     2161 ggtctcgcgc tgggcgagcg gcgaggtggg atccatccgt caggagagcg aggagcgcat
     2221 ccgcacggcc tacgaggtgg cccagctcct cgtgcagttc gactcgccca ggatagtgaa
     2281 ggcctggttt ataggtctca acccccagct cgacgacgtc tcccccgccg agacgatccg
     2341 cgaagggaag ctgaaggaag ccaaggctgc cgcgcgcgcc ttcgtcgctg gtggctgatg
     2401 ccccactgcc ggccgtaagg ctgcccgagt tggtctaccg tgtggcgcgc ggcctcgacg
     2461 ccttcgcgcc gccggactgg gcctacgcgc tcccggacgg caccttcggc aaccgtttcg
     2521 acgaccctgc ggccgccgac aagggtgtgc cggaagagga acgctaccgg gcggtttact
     2581 gcgctaccga gcgagcaggc gcgttcgggg agaccatagc gcgtttcagg ccgagcatgg
     2641 agttgctcgc tgggcttgag gaggtcgagg acgacgagcc tctggacccg gagcttcgcg
     2701 gaggggttgt ccccgaggag tggcgtctga gccgccgcgc gcgggctcca ccaggctcgc
     2761 ccccggcctg ctgttcgccg atctcaccgc tccagagacc atgcgtgtcc tgcgaaagaa
     2821 cctagccgcg gtggcggaga agctcggcct cgaggacctg gacctgagcg cggtgaccgg
     2881 accgcaccgt cgcctgaccc aggaggccgc gagatacgtc tacgatctgg aagattcttc
     2941 aggtagtctc gccttcgctg gtatacgcta catctcgcgt ctgaacccat cctaagagtt
     3001 gtgggcggtc ttccacgacc gcatgcgcca cgaaccgggc gaggtggccg agccgatcag
     3061 ggcggaagat cccggcctcg tggaggctgc caccctcctc ggtcttcggg tagagtaacc
     3121 cgagttgccc cctatcccag taaacaatat cctcccgatc cgaaggccgc ctaaaacctt
     3181 ctctggatcg ggaggacgag atggaagata ccataaccac cacctactac ctctgcgacg
     3241 agttcttgaa ggccgtcggc caccgcgacg acccacaagc ccgtctgtcc accgccgagg
     3301 taatgaccgt acccctggtg gcctgcacct tcttctccgg caacctcgag gcgagccgcg
     3361 cgtttttgga tgggtacgga tacatagaga agacgatctc caaaagccgt ttcaaccgtc
     3421 ggctgcatgc catagactcc tgcctctggg agggtctctt cgcactcttg gctgaagtct
     3481 tcaagcatga gcactccgag cgaacctacg tggtgcactc cttgccggtc gccgtgtgtg
     3541 acaacatccg catccgccgc tgccggctct atcctccgga agaacacgga ggagcgttcc
     3601 gcggctacat cgccagcaag cggcgctact tctacggcct gcgcgtgcat ctggtggtca
     3661 cgggggcggg cgagccggta gagttctcct tggcggcggg cgcgcaagcc gacgtgagcg
     3721 tgttcaagaa cctgggatta gatctgccag taggctcgac catcttggcc gacaaggcct
     3781 acaccgacta cgactacgaa gatctcctgg aagaggttgg cctgcacctc aaggcccagc
     3841 gcaagaagaa ctccaagagg ccgatgccta tgtgggagga gttcttgggc aagtccatcc
     3901 gccaatacat cgagacggtg ttcagccggc tgagcgcctt attcgccagg aagatccacg
     3961 cggtcacacc gaggggcttc gagctaaaga tcgcctgctt cctgctagcc ttctccattc
     4021 agtgccttta gggggcaact cgggttacat tagcaggcga gcgtcgagag gggcttacaa
     4081 ttcaccaagc tcctctaggt cctctaggct cccggacgcg cgtcggcggc acgctcgctc
     4141 ttggcgagcg atcccccgaa taccttgatg agctgctccc tggccctgtc caggtgctca
     4201 tcgcaagcct cctccgccct tctcgcgtcg agcgactcta tggcccggaa cagcttttca
     4261 tgatcgccat agagatcctc gagcgaagac cgcgaggtgt agatcttgtc cacctcgagc
     4321 ctgaatatca ggccgagtac ggcggcgtag cttacgaaga cgcgatgaag cctcccgttc
     4381 ccgctcagca ggcagagccg ctcgtggaag gccagatcca gcttcgtgaa gagggttcga
     4441 tctccttcgt cggccgcgcg ctcgagctgg tggaggatct cgcgtagctc gtcaagggcc
     4501 gcgagatcct tgctggtaat aatcaggcgc gccgctcttc cctcgatcgc ggcgcgcaga
     4561 tcgtagatct cccgcacgtc ctcaatcgta agatcgatga caaaagaacc acgccgcggc
     4621 tcctcccgca cgagcccctc agcccgaagc tgcctgagcg cctcccttac cggcccccgg
     4681 ctgatctcga gccgcttcgc gatcttcgcc tccacgagcc gctcccccaa tccgaaatca
     4741 cccgcaagga tcctctcccg cagcaaatcc gtcgccctgt ccgagagcgt ttcggcccga
     4801 cgcaacggcc cgagactgcc gttgtccccc acctcgatgc ccccagcctc cgtcatctat
     4861 tactcctcct acgtacgagc cacacatagt attacaaatc ttttttagta aactgttgac
     4921 agtttacagt aaacatttta ctatgctctt cggtacgggt caacgggtcg aaggaggcag
     4981 gaaccatgac gctctcgccg ggagacaatc ccgaagcacg cagccggttg gaggagcttg
     5041 gctattctca ggagctcaag cggcggctca agatgtggca cgtggtgggg cttgcgatgg
     5101 ccgacgtctc gccgacgatg gcggtgttgt tcctcacggc gggggtcttc ttcgttggcg
     5161 ggaccttcgc gctgggcgct aacctgatcc tggcggtcgt ggtggtgttg atcgctctgt
     5221 gcctggcgga gttggcggcc atgtacccta tagcgggcgg gatgtactcg ctggtgcgtt
     5281 atgtgttgcc gggcccgatc acctggatca cgatgtttaa ctacctgctg caggggatcg
     5341 tgatcccggc gagcatcgcc ttgggggtcg cgaccttcct tcaggacctc gtccccgccc
     5401 tcgacgcgtt gcccgtgccg ctgctcgccc tgatctccct ggcggtcgcc actgtcatag
     5461 ccctgatgac catcgaggtc ggggcctggc tgaccttcat catggtggtc gtcgagctcg
     5521 tagtcctcgg cctggtcacc gtcgccgcct tcgccaaccc gaatcagaac ctcgccgagg
     5581 tcgtcttcca tccggtcgtg ttgcatgatg gctcgctcgc ggcggttggc ttcggggtca
     5641 tgctcgccac gctggcgccg gccttcaacg tcatcaatgg ctacgacgcc actctggggt
     5701 tctcggagga gatgatcggc ggggagcgct cgatcccgaa ggcggtagtc agcgcggccg
     5761 ttctggcctc gctgttcatc attgtgcccc taatcgccgc cgtggtggcc gcgccggacc
     5821 tgaaggcatt cttcaacgcg gacgcaccca tcgtctactc cgtggagcag gcgatgggcc
     5881 ggggggcggg catgatcgtc gacttcggcg cctcgatcgc cctcttcaac gccatgctct
     5941 ccctgctcat gtacttcggc cgcggtgtgt acacgaccgg ccgcgacggc atatggcccc
     6001 cgcccgtgag ccgcgtcctc gcccgggtga accgcttcgg ggtccccgcc gccggcgtcc
     6061 tcgctcttgc ggtgcccgcc gcgatcctca tcttcctcac ggcgctcgac ttcctgatta
     6121 tcttcgccgg caccatgatc gccgccgtct acttctgtat cggccttgcc gccctctgga
     6181 gccgcatctc gcagagggac gagccgcgcc cctacaggat gccgctctgg ccgctcaccc
     6241 ccatagtggt ggtcgtcttc acgggcgtcg cgctggtgct gcaggggagc gagtacctga
     6301 tcgccgagct ggtcctcgcc gccgtcgcgg tcgcgctatg ggcggtctcg aagacctggt
     6361 ccggcgccca gaggtccacc gagaccccgg ccgaggccga gcgttgaaag acagggtcgt
     6421 cgcgatcacg ggcgccgggc gcggcatagg gctcgccacc gcgcgccgct tcgccgagaa
     6481 gggctgccgg ctcgccctca accactggcg cacgggcaca aaagccctcc aggaggtcgc
     6541 gggcctcgcg cgcgagaacg gcggggatgc gtttctcgtc gaggcggaca tcggcaggcc
     6601 cgaggaagct gcgcggttcg tgcgggagtc ggaggcgcgc ttcggcggga tcgacgtcct
     6661 gatcaacaac gccggcatcc tccgctccac cccgacccac gaggagacac aggctaattg
     6721 ggatgaggtt atctccgtca acctcggcgg gacgtggtcg tgcctcaggg cagcgctgcc
     6781 tggcatgctg gagaggggga gtggacgcat catcaacgtg agctccgagc ttggcatcat
     6841 cggatacccg acctacgcgg cgtactgcgc ctcgaagggc gggatcatcg cgctcacgaa
     6901 ggcggtggcg aaggaggtgg ctccccgcgg cgtcctcgta aactccgtcg cgccggggcc
     6961 agtggagacg gacatgttga tccacgacac gatcgagtac aacgacgaga cgcgcgagca
     7021 gatcccgctg aggcgttttg gcaggccgga ggagatcgcc gccgccatcg agttcctcgc
     7081 cggagaaggc ggctcgttca tggtcgggca gatattgagc ccaaacgggg gcacggcgat
     7141 ctagagtctt gcaagagaaa ggagaacccg atgacgacca cgatggtgac gggcggactc
     7201 ggatacgtgg gcaggcatct cgtcgcccgg ctcgcggagc gcggggagcg ggtggtcagc
     7261 tacaaccgcg actacgccga gggaggagaa gagaacgtaa cctacgtcca gggcgagctc
     7321 tacgacctgc cgcgcttcgt cgacaccata gggcgctacg aggtagggcg cgtaatccac
     7381 accgccgcca tgtcccaccc ggacctctcc atagacctgc cgatcaccac gtttaccgcc
     7441 aacgtcgacg gcacggtcca cgtcttcgag gcagcgcgca tgagtggtgt ccggcggatc
     7501 gtcaacttct cctcggagac cgcctacggg cacgtcgagg ccgagccggt taccgaggac
     7561 gcgccgctcc acccgaccac gccttacggt gtgacgaagg tcgccacaga gctgctcggg
     7621 caggtctaca gcgacctgta cgggctggac gtgatctcgc tcaggatcag cgaggtgtac
     7681 ggccccggaa acagaatgcc ccagttcctg cgggagatga tcaactccgc cttgcaaggc
     7741 gagccgtacc ggctggagaa agggggcgac caccgcttcc agttcatccg ggtcgaagac
     7801 gtggcgcgcg cggcgatcct ggcggccgac gccgaacgga cgagccggcg cgtctacaac
     7861 atcacgggcg ggagccaggt cacgctggcg caggccgctg agatcgtccg cggtgagata
     7921 cgggaggcgg acgtggagat aggtccgggg catctccacc tggaccggca gggaccgtac
     7981 gatattaccg ccgccgggcg ggatctcggc tacaggcccg agtggtcggt agagtgcggg
     8041 atccacgact acgtggactg gcttcgcgac cacccctact agaggagcgc cgatggaagg
     8101 aaaccccatg gaacagcgaa ccaggctcat ggctgagatg acgtggccgg aggtcgaagc
     8161 ggccatccgg cggggggcgg gcgtcgtgct gcccgtcggg tcgacggagc agcacggcca
     8221 ccacctgccg ctcgctaccg acgccatctt gccgaccgag ctggcgctcg ccgttgccgg
     8281 gccgctggat ctcctcgtcg ccccggccgt gacctacggg tatagatccc gccccctgag
     8341 cggcggcggg cagggcttcg tcggcacgac ctcgctccgg gcaacgaccc ttatagcgct
     8401 cgttgaggat gtcttgcgcg agcttattcg gcagggcttc aaacggatcg tgctcctcaa
     8461 ctggcatatg gagaaccaga acttcgtcta cgaatccgct tatttggcgc tcgagcagag
     8521 ccgcgagtct caggctcgag tcctcgtagc agagctcgcc ttcacggacc tctcatccga
     8581 aaccatgcgg gcgctcttcc cggagggttt cccgggctgg gacgtcgagc acgcctcgat
     8641 cctggagacg tccctgatgc tacatctcca taccgagatg gtcctcttcg accgtgcggt
     8701 agacgacgcg gcgaaacggc acccctggta cgatgtcctg cccgtgcccg cggacttcgt
     8761 tccccggtcg ggcaccctct ggaaggccac gcaggcgagc gccgagaaag ggcgaatggc
     8821 ctggcgcgag atagtcgagc agttgcgcga agccatctcc gcggagctcg ggtagggtga
     8881 ccgagtcgac ctccgccctt cggtccggcc ttagcagtgg agtgcaccca tttcaccgga
     8941 catctccgtt agtcaacttc tctggagatc ggatgtggag ttgcatccgg accgaggagg
     9001 agagattgtc caattcgcgc agaaaccaca ccagagagtt taaggggcca gcccaaaggc
     9061 gccacgaatg cctcgtcgag cggtggaagg agatccgaaa gctgcacctt gccggggcca
     9121 gggtgaagga catagcggag tgggtcggca cgagccagag caccgtctac cgctacaggg
     9181 agctggcgga gcccccgccg cggcccagct acaggagaag ggccagcgtc ctcgatccct
     9241 aactgcccta cctccttgag agatggaacg agggctgccg cagcgccaag cgcctccacc
     9301 aggagatccg agagatgggc tccgccacag cgtggacacc gtaaacaggc tgctttcaag
     9361 cttccgtcac acggagggcc gggcagaggg ttgccccacg cgccgagggc gaagaggggc
     9421 tacatcgccg gcgcctcccc gaccgccaag aacgtcgcgg cgcttttcat gcggcgcgaa
     9481 gagatgctga acgaggagca gaaggcgtac ataaagaggc tgtgcgcctc cgatgcggcc
     9541 ctcgcagacg cgcggcgtct aacccaaagg ttcgcgacca tggtcagtaa cctcgaaggc
     9601 gagaatctag acggttggtt ggagaaggcc gcaggatgcg aggcgacggt gatgaagagg
     9661 ttcgcgtccg gcctggagaa ggaccttgag cggtgcgcgc gggtcttccc gaggagtggc
     9721 cgaacgggcc ggtggagggc ttcgtgaaca agctcaagtt gctaaagcgc cagggctacg
     9781 gcagggcgga cctctacctg ctgagggtga ggaccctggc ggcctgatcg tcgctgcggc
     9841 gggccgagcc tccgagccat acgttcacct aaagttcggg agaacccaca cttcatgagc
     9901 aggtctgacc atcctaccga ggatcttcca aagacaccct tctacctact tgatcgtgaa
     9961 gtaccagtac ctcgctccca cgttgccagc ggcatcggtc gctaccacct tcaccgtctt
    10021 cttgcccctg gcgagcttgg gcgagttgta ggtgagaacg tcggtcgagg cgctgtagga
    10081 gtacttggtg gcggagatcc gggccccgtt tacgtagagt tttatgttcg atttttgaag
    10141 gttcgtcacg ttgtctttga tggtagcctt gatggtgggt gtgcggtccg tgattgtaga
    10201 cgcaggcttc ggtgacatcc tcgttatggt cggcttgatg gtgtctaccg tccagctacg
    10261 cgccgccggg gtggcgtccg tgttgcctgc cgcgtcggtc gccgcgaccc gaaaggtgtg
    10321 ggagccattt ggcaggcttg tgtagctctt tggagacgtg caggcgctca aggccgcgcc
    10381 gtcgagactg cactggaatg tggcgttagc ctccgaggag aaaccgaagc tggcggaggt
    10441 gctgttggtg atactggaag gaccggtggc gtcgagggtc gtctcgggcg cggtgatgtc
    10501 gtttatggtg acatccctgg tgtactcggt cgtcccgccg tccttgtcta tgatcttgcc
    10561 ccggaccgtc tggctattac cgtccccggc cgtgcagaac gcctggctgg agctttggga
    10621 ggtggtgtag ccctggccga ggccgcagtc gaaagcgtag gagaatccgg agttggtatc
    10681 cgcagcggag ggatctgacg gctcggtaaa cgagagaggg atctgggtgt ccggattgac
    10741 cgagctcggg gcgttgaagt ttccctgcgg ggccacgttg cttacgccca cgctgttcgc
    10801 gtactcggag acccctccgt ccttgtcctt tatcttgccg ccgacggcga gcgtagccgg
    10861 accgtcgcct gcggggcagc ttgcgctgct tgaggagccg aaggtgccat agccgctacc
    10921 gcagtcgaag gcgtaggaga agccggcgtt cctatcggcg gtcgagggat cgagcgggct
    10981 cgtcagggag agggtgaacc ctccggaccc ctcgtcgacc gtcgtggtgg agacgttgaa
    11041 agtggctgta ggagccacgt tgtttatggt cacggagccg gtgtacgccg ggttggtcgt
    11101 gccggccgga caactaaaca gtgagcaacc gcttggcgtg gtgggctggc tatccgtgaa
    11161 cccgccgtcc ttgtcctgga tcctggccct taccgtggtg ctgccgttgt cgtcggtcgg
    11221 gcagttggcg ctgttgcccg aacccagcgt tccgtagtga gacgtccaga acaaaaacgt
    11281 attaccaaaa acccccgcgc ctgattcaag agagtcgcgg caactgaagg cgtaggagaa
    11341 gccagcggcc gtgtcgacgc ccgatggatc ggtgggattg gtcaaggaga ggccgatcgg
    11401 gccgccttcg tccaccgaag tcggatagcg gaaactgcca ttcggggcca cgttgttgac
    11461 cgcaagcgag aaggagacgg tcgaagagtc cgtgccgtcc gaggcggtga tggtgaccgt
    11521 cgcgttctgg gaagggccgt cgctcgttcc atacgaccag gcccaggtgc cggggcccgc
    11581 ggcgtccggg acgacgtttc cggggccgga ggtcacactg agggttacag gatcgccatc
    11641 tgcgtcggag taggtgccgg tattgttggc cgtctggctc tcgttgatgg tgacggtagc
    11701 gttgttggcg gccaccgtgg gggctgtgtt cgtgccgccg ccggggtcgc aggtgtcgca
    11761 gggcaggggt acagccctcg ccggtcccgt catgaggagc agtcccagca ctatggctac
    11821 gagcagcccg gcggcgaaga cggtcatact ccggcgcgtc cgcgatattt tgaatctaat
    11881 aatcgtcatt tccactcctc tccggccgac tcctggtccg ccccatctcg tacctctcga
    11941 tcctcatcct acttggacag cgtctctcaa tcgttacgga aagcgttacg gttgagatat
    12001 ttgggaaata tcttgctctc gcatctccta tctggatcgg caaaaccttg accttcgaag
    12061 tctcgaaaac ccctgaagcc ggttgcgcgc cgctcgacgc gctaatcatg agatcgccgt
    12121 agaggttgca tcttctagct ggctctcacc cgaagatagt gtgggtggcg tacgtatacg
    12181 tattcttcgt aggaaagtag gtagatagta ttcccccggt ggtgagtagc accgcgaaat
    12241 atcccgcctg gattgctacc atctctccac aaccaattcc aaaccactct ccctcccctt
    12301 taggagatgg acgggatgat cagtcccgcc tagagctaca tatattaggg ttattggcac
    12361 atccactgta aaacctgtag cgccaagctg cggattcccc gaaagggcga ggaggaaccc
    12421 tgccatttcg gccaaagatc cgcccggctt ggcgccgcaa cgctacggga gagtatgagg
    12481 ctgacagggc atgttggata ttggtcacct ggcctatatt tatgacgtcc tttatgacgt
    12541 cccctgattt ctccctgtcg ttcgggccct gacctcgatg gacaagagac cacgtacaga
    12601 agaggtaatg aatcacgaac gccggccacc cgcgatgccg cgggtggccg tgatgacccc
    12661 gggcgttggc taacgtacat ccaatgcgcc tttcggatca cttctaaatc gccagcaacg
    12721 tatgggagac cgcaaagatc actcatttga gggatgcgtc ccaccctcgc gcctacaaga
    12781 cttagcacgt cggccaacgg gggcggtgcg tacttccacc tggccctcac atcagaagaa
    12841 ggagagtcca tgtcagcgag aacccaaacg cgcagcctgg tgttggcctt tatcgccctg
    12901 ggtatcgcag gggccctgct cgccgtgatc gctgttgcgc cgcccacact ggcccggtcc
    12961 gcccgcccga acgaaggatt gcgggttctg agccccggcg agagaccttc aggatcgcga
    13021 acaccgggga gcctgtccgc gttgcagaag cgggcgctga tcaaaggata cctggtcccg
    13081 gaccagcagg gctacgagct ggccaaggcc gcggcggacc ggaaggccgc ggcggggacg
    13141 ggccaggagg cgccgttcgc tccggcctcc gcggccccca cctcatttcg cagctggcag
    13201 ggcgcccgcg acgccggtgg cgccccatcg gactcgacgg gtgctatcgg tccgggccgc
    13261 tatgtagagc tggtcaactc caggtacgcc atctacaacc gaaccagcaa cgtcccgatc
    13321 aaccagggca gcctcaacga cctggtcggt gcggcggcgt tggaatccgt gttcgatccg
    13381 cagatcatct gggacccaac caccaaccgc ttctactacg cggcggacga cgtcctggac
    13441 agcgccaaca acttcctggc cttcggcttc agcaaggatt ccacgcccga tgcgggaccc
    13501 tcggactggt gccagtattt cgtcgcttac ggagccgact tccccgacta cccgaagctc
    13561 ggcgacacca gggacttcgt gatgatcggg tcaaatgtct tccccgccgc gggcgggttc
    13621 cactccagcc tggactggat ccgcaagccc ccggcaggcg ccggctgtcc gaacttgagc
    13681 agcggggtaa agaccgatct gcggaactcc gacggcacgc aggcgatcac ccccgtgccg
    13741 gccaatcaga ccgacacgag tcccaccggc tacgtcgtgg ccgaaccagc ctcggttcca
    13801 aacggcgccg gacccgccgg cttcctctcc ctcttcacgg tgagcagcaa taccgatggc
    13861 accctcgccc tgagcgctcc caggaacgtg accgtgccgg gctacgacct gcccgcaaac
    13921 gccccccaga gggggacgac aagaaggctt gacacctccg acagcaggaa cacccaggcg
    13981 gtctcggcca tagacccctc ccgcggtacc ggcggtctga cggcgctgtg gacccagcac
    14041 acggtcttcg gcggcgccgg agcgcaggtg agatggtatg agatcaaccc ggcctccgcc
    14101 acgttgttcc agagggggac cgtgagtggt tcctcggcga atggcgtctt cgacttcaac
    14161 ggagccatct ccccggaccg caaggtactc ggcaccacaa gaaaattcgg cagcagcatg
    14221 atcctgggct acaacagctc ctccgccacc cagttcccga gcgtccagat gatctccaag
    14281 gttggttcca acgcggcaag cgcggggacg ctagtcaagg cgtccgccgc ctcgctgcaa
    14341 aacttcgatt gttcggaccc caacttcccc ggtgtctgcc gctggggaga ctactcggcg
    14401 gcgacgccgg atccaggctc gtccaccacg acgacccacg gacaggtgtg gctcac
//
LOCUS       DASSMB010000088         8850 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_525619, whole genome shotgun sequence.
ACCESSION   DASSMB010000088 DASSMB010000000
VERSION     DASSMB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525619"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFJ72_05655"
     CDS             complement(<1..812)
                     /locus_tag="VFJ72_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7478986.1"
                     /translation="MRPEDIDGLTVGGLLDLVTGRRPDDEALVYADRDLRYTYREFNE
                     RVEECARALMALGIEKGDHVAVWGQNVPEWVTLQFATGKVGAVLVTINPAYRARELKY
                     VLDQSDSAALFLTSGVRGASFLEILQGAIPELADSEDEELSLADLPFLKHAVLMGGGA
                     TEGLPVLTYEEFLTRAESVSQDELKGRQASLDAEDVINMQYTSGTTGFPKGVQLTHTN
                     IVKNAYLIGECMKLGPEDRVCIPVPFFHCFGCVLGTLNTVTHEATMVPVEQF"
     gene            1015..2781
                     /locus_tag="VFJ72_05660"
     CDS             1015..2781
                     /locus_tag="VFJ72_05660"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7478987.1"
                     /translation="MDRKKRSFSSRKARRQRFKALLLLAAVLLTSLAAYFSVRSLDRA
                     APPEAGLSQDEPYTRLSQEPLTFRPGAPDPPELTLAGYAYKSVAPELPGIGPESVRYA
                     QQSVFDPSWASVRIAAPGHRRDDYYAVFLHKNGKEWKAERSVLIEDQEFPKDVTALLG
                     GIPEDLVNPLFPPDKAQEKSNDPDGRAVQVMERATGEEDWKAADTENAGPYHRVSVER
                     KGKKDLRTNVYLSGRGEDLTVVGIGEKLTNVEAPGFPPDLVKPAVMAAPDPARVAPPD
                     PVYDGKVDMDRVHPGMAEALKTVESYPGTVGFYALDLKSGAGYGVRPDEPFFSASTIK
                     LPVMVAVYRRIDEGKLSYSDAFETTKEDWAAGAGWLRWDTPGAQTTVEDSLWLMITQS
                     DNVATNALVRKVGGPEYVNQVARSLGARDTVLFTKLSSERAAVPSLDNRTTPKDMALM
                     LQEITSHKAASDFACDEMLDLMRQNNLEYWMEAGVPEGMPVANKGGWLDATYNDVGFV
                     EYKDRPYILATFSKYGADKMEQGAQPIQDVSRAVFLAQSGQTVEQYEKEQKKKLKQKI
                     EEQRRNAEKQQEDSPKNPQKTP"
     gene            2858..3874
                     /locus_tag="VFJ72_05665"
     CDS             2858..3874
                     /locus_tag="VFJ72_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7478988.1"
                     /translation="MSQETNGGAKATIIDVARRANVSKSLVSLVMRGSPNVSPARREA
                     VLRAASELGYRPNALARGMRGRSYTIGILLSDLHNPFFAEVVDGVEEGLGSTEYRALL
                     GSGGRDPDRELEAIQSMLDRQMDGLILISPSIQERDIIATARQAPTVLVGGRIEDPAL
                     DYVVNDDHAGAALAVGHLADLGHERIAHVSGGDGAGARDRQRGYQETMRLRGLEKHIR
                     VAPGSYTDAGGYGGARRLLSESPQPTAIFASNDLAAIGALTAVREAGLMVPGDVSIVG
                     YDNTYLAALSHISLTTIDQPRLRMGALAVQLALQRIESGRAEPRREVLMPTLVARSTS
                     APPA"
     gene            complement(3887..4903)
                     /locus_tag="VFJ72_05670"
     CDS             complement(3887..4903)
                     /locus_tag="VFJ72_05670"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7478989.1"
                     /translation="MSVKVGIVSFAHVHAPQYASILADLPCAEFVGIADDDAARGRES
                     AEQFGVRFFEESAELLDAVDAVVVCSENRHHLRDVVPALRSGVHVLCEKPISTTVEDG
                     RAMNAASEESGAQLRIAFPVRYLPSVRRARDLVRAGTIGRVLAVSGTNHGQNPGGWFL
                     DPDLSGGGAVMDHTVHLADTLRWMLGAEASSVYAEVGTFFGTKGIDDAGILTLELEGG
                     SIAPGIFATIDPSWSRGAGYPTWGDLTLRIAGTSGVLEVDGFAERLTTFDHEAGNVAW
                     TPTGEDMNVAMLADFLRGVESGEPAGASGLDGLRALEIVLAAYRSGRDHRPVEVERSP
                     AAGG"
     gene            complement(4900..5886)
                     /locus_tag="VFJ72_05675"
     CDS             complement(4900..5886)
                     /locus_tag="VFJ72_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7478990.1"
                     /translation="MGGVKVGVVGAGFMGSVHLSAYAKMPEVEVVGVADTDPENATAG
                     AALVGARAYSTYEELVRSEEVDVVDVCLPTGFHRDLALAVARDGKHLILEKPIARTLE
                     DADEILGAFEEGDARLFVGHVVRFFPEYVRIKNMVEDGSLGKVGVARTSRRSPLLMGW
                     NDWYADWRVSGGVLVDLLIHDFDFLRWCFGEVERVYARGVLGREHNRLDYALVTLRFE
                     GGEISHLEGHWGYPGAFNYSIEIAGSKALVTADSTEPSSLRLTGGAPESGGLAPVGKS
                     PYQSELEHFVRCIEGGEEPIVSGKDARAALAISAAAIESIRTGEPVRPGGAR"
     gene            complement(5893..7239)
                     /locus_tag="VFJ72_05680"
     CDS             complement(5893..7239)
                     /locus_tag="VFJ72_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7478991.1"
                     /translation="MEANDGASSARTISRRDFLKVGGAGLAAAALLGVAGCGGGQEQG
                     GGGATELVFTASPDDTETASKLVDKFNEQNKGKYKVSFRPGNADTGQRFDALRTQLQA
                     GGENLDVILGDVIWTAQLAENGWISDLSDRFTEDMRGDYLPGSVEAITYKGKTFGMPW
                     FTDTGLLYYRKDLLQKSGFDGPPKTWDELKQMALKVKQDSGIKFGYVFQGANYEGGVC
                     DGCEFIWTHGGEVLDPQNPDRVLVGQPEAIAGLTTYYSMIAEGVAPKAVTTYKEDESA
                     GAFLNGDAVFLRNWPYVYAIISDPSESKVKPEQVGVSELPSADGEPGNGTVGDQPLYI
                     NATSKNPDAAWEFIQFMTAPEQQKFRIIEGSYLPTLKALYDDAEVKKKVPIAALAKDA
                     LQHTRPRPVSPYYADMSLQMSKHFNAALKGDETPEEAARTLQDKLEEYIKQGKEEG"
     gene            complement(7260..8060)
                     /gene="iolB"
                     /locus_tag="VFJ72_05685"
     CDS             complement(7260..8060)
                     /gene="iolB"
                     /locus_tag="VFJ72_05685"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET7478992.1"
                     /translation="MPDLIVSSNPEPDANGSVVKVTPESAGWDHVGFEVLRLASGHSV
                     ERHALGEEICLVSLSGQCSVVAGEERWEEVGGRKSVFDGPPHALYLPPGTGYRIGAVT
                     DLELAVCSAPAESGAEPLLVRPEDIEVEVRGYGNLEREIRPILMDDRPAESLLVVEVI
                     TPNGHWSSYPPHKHDRDDPPRERYLEETYYHRIKPEQGFALQRVYTEDRTLDETLAFR
                     DGDVVLVPRGYHVVSAPPGYDLYYLNVMAGPVREWRIHNDPDHEWLLK"
     gene            complement(8076..>8850)
                     /locus_tag="VFJ72_05690"
     CDS             complement(8076..>8850)
                     /locus_tag="VFJ72_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7478993.1"
                     /translation="SEEAGRAWDLEVERLYTLDHGPLPSQGEVIGAVNSFSEPEDVVV
                     CAAGSMPGDLHKLWRTRDPKGYHVEYGYSCMGYEIAGGLGIKMAAPEREVYVMVGDGS
                     YLMMNSEIVTSIQEGYKLTIVLVDNSGFASIGALSRSVGSQGFGTQYRYRKDGSLGLD
                     TEKAPGEVLPVDLAANAESLGAHVIRTGTVDELKEALAQAKSVDRTVVIHIPVDRYEG
                     VPSYESFWDVPVAEVSEMDTVIAARKEYDENRKAERHYL"
BASE COUNT         1532 a         3081 c         2749 g         1488 t
ORIGIN      
        1 tcgaactgct ccacgggaac catcgtcgcc tcgtgggtta cggtatttag cgtaccgagc
       61 acgcacccga agcagtgaaa gaacgggacc gggatgcaca ccctatcctc cggcccgagc
      121 ttcatacact cgccgatgag ataggcgttc ttgacgatgt tggtgtgggt tagctgcacg
      181 cccttcggga atcccgtggt cccagaggtg tactgcatgt ttatgacgtc ttcggcatcg
      241 agcgatgcct gtcgtccctt cagctcgtcc tgcgatacgc tctcggcgcg ggtcaggaac
      301 tcctcataag tcaggacggg cagcccttcc gtggcgccgc cgcccatcag gacggcgtgc
      361 ttgaggaaag gaaggtccgc cagggacagc tcctcgtctt cagagtctgc gagctcgggg
      421 atggcgcctt gcaggatctc caggaagctc gctccccgga cgccgctcgt caggaacagc
      481 gcggccgagt cggattgatc caggacatac ttcaactcgc gggcgcggta ggcggggttt
      541 atggtgacga gcaccgcccc gaccttgccg gtggcgaact gaagcgttac ccactcgggc
      601 acgttctgtc cccagacggc tacgtggtcg cccttctcga tgcccagcgc catcagggcg
      661 cgcgcgcact cctctaccct ctcgttaaac tcgcgatagg tatagcgaag gtcccggtcg
      721 gcgtagacca gggcctcgtc gtccgggcgg cgtccggtga cgagatccag cagccccccg
      781 accgtcagac catctatatc ctccgggcgc atatggacct cctgtctccg attcccctcg
      841 gtaagccgga gtgtagcagt tgctccaagc cgcgcaacat ccgtacccaa cggcgattcg
      901 tattgggccg gtgatcggag acgttgtaca cttcgtattc tcgacaggag agtacaggct
      961 aatgcggcgc ggcgggaggg actccgcgcg ggatgggcgg ctacgattct tcaaatggat
     1021 cgcaagaaac gcagcttctc cagcaggaag gcgcggcggc agaggttcaa ggcgctgttg
     1081 ctgctggccg ccgtcctcct cacctcgctc gcggcctact tcagcgtgcg gtctctggac
     1141 cgcgcggccc ctcccgaagc cggcctctcc caagatgaac cgtatacgcg gctctcgcag
     1201 gaacctctca ccttccggcc cggagccccg gacccgccgg agctgacgct cgccggatac
     1261 gcctacaaga gtgtcgcgcc ggagttgccg gggatcggtc cggagagcgt gcgctacgcg
     1321 cagcagagcg tcttcgaccc ttcgtgggcg tcggtccgca tcgcggctcc ggggcacagg
     1381 cgggacgact actacgccgt cttcctccac aagaacggca aggagtggaa ggctgagaga
     1441 tccgtcctga tcgaagacca ggagttcccg aaggacgtga ccgcgctcct cggcggaatc
     1501 ccggaggatc tcgtcaaccc gctctttcca ccagataaag cccaggagaa gtccaacgac
     1561 ccagacgggc gagccgttca ggtgatggaa cgcgccaccg gcgaggagga ctggaaggcg
     1621 gcggataccg agaatgccgg tccctatcac cgggtatccg tggagcggaa ggggaagaag
     1681 gacctccgca cgaacgtata cctcagcgga cgtggcgaag acctgacggt ggtcggtatc
     1741 ggggagaagc tgacgaatgt cgaggcgccg gggttcccgc ccgacctcgt caagcccgcc
     1801 gtgatggcgg cgccggaccc ggcgcgggtg gctcctcccg acccggtcta cgacggcaag
     1861 gtagacatgg accgggtgca tccgggtatg gcggaggcgc tcaagacagt ggagagttat
     1921 ccgggtacgg tcggtttcta cgcgctggac ctcaagagcg gggcgggata cggagtgcgg
     1981 cccgacgagc cgttcttctc ggcctcgacg atcaaactcc cggtcatggt cgccgtgtac
     2041 cgccggatag acgagggcaa gctgagctac tcggacgcct tcgagacgac gaaggaggac
     2101 tgggccgccg gagccggctg gctgcggtgg gacacgcccg gcgcgcagac caccgtcgag
     2161 gactcgctgt ggctcatgat cacgcagtcc gacaacgtcg ccacgaacgc cctcgtccgc
     2221 aaggtcggag gccccgagta cgtgaaccag gtggcccgct ccctcggcgc gcgggacact
     2281 gtgctcttca cgaagctcag cagcgagcgg gcggcggtcc cgagcctgga caaccgcacc
     2341 acgccgaagg acatggcgtt gatgctgcag gagatcacat cccacaaagc cgccagcgac
     2401 ttcgcctgcg acgagatgct ggacctgatg cgccagaaca acctggagta ctggatggag
     2461 gccggcgtcc ctgagggcat gcctgtcgcc aacaagggcg gctggctgga cgcaacctat
     2521 aacgacgtcg gcttcgtcga gtacaaggac cgcccgtaca tcctcgccac ctttagcaaa
     2581 tacggcgccg acaagatgga gcagggcgcg cagcccatcc aggacgtctc ccgcgcggtc
     2641 ttcctcgccc agtccggcca gactgtggag cagtacgaga aggagcagaa gaagaagctc
     2701 aagcagaaga tagaagagca gcgccgcaac gccgaaaagc agcaggaaga ctccccgaaa
     2761 aacccgcaaa agactcccta acccggcatc cgcctcccga agccgaacaa acggttaaga
     2821 tagacgggaa ttggacgggc gcgagagacg gtcgaaggtg tcccaggaga caaacggcgg
     2881 cgcgaaggcc acgatcattg acgtcgcccg gcgggcgaac gtctccaagt ccctcgtctc
     2941 gctcgttatg cggggctcgc cgaacgtgag cccggcccgc cgcgaggcgg ttttgcgggc
     3001 ggcttcagag ctgggttacc ggccaaacgc cctcgcgcgc ggcatgagag gccgttccta
     3061 cactatcggg atactcctct cggacctgca caaccccttc ttcgccgagg tcgtggacgg
     3121 cgtcgaggag gggctcggct ccacggagta ccgggcgctg ctgggctccg gcggccgaga
     3181 cccggaccgg gagctggagg cgatacagtc catgctcgac cggcagatgg acgggctcat
     3241 cttgatctca ccgtccatcc aggaacggga catcatcgca accgcgcggc aagcccccac
     3301 cgtcctcgtt ggcgggcgga tcgaagaccc cgccctggac tacgtcgtga acgatgatca
     3361 cgccggcgcg gcgctggccg tcggacacct ggccgacctc ggccacgagc gcatcgccca
     3421 cgtctccgga ggcgacggcg cgggggccag agaccgccaa cgcggctacc aggagacgat
     3481 gagacttcgc ggactggaga aacacatccg cgtcgctccc ggctcctaca ccgacgccgg
     3541 cgggtacgga ggagcgcggc ggctgctctc tgagagcccg caacccaccg ccatcttcgc
     3601 ctcgaacgac ctcgccgcga taggggcgct caccgcagtc cgcgaggccg gtctgatggt
     3661 ccccggagac gtctccatcg tgggctacga caacacttac ctggcggctc tgagccacat
     3721 ctccctcacc accatcgacc aaccccgcct gcggatgggc gctctagcgg tgcaactagc
     3781 tctccagagg atagagtccg gacgcgcgga gccccgccgc gaggtgctga tgccaacact
     3841 cgtcgcccgc tcgacctccg ccccgccggc ctgagagagc agcggttcag ccgccggcag
     3901 ccggcgagcg ttctacctcc acgggcctgt ggtcccgccc cgagcggtag gcggccagca
     3961 cgatctccag cgcccgcaac ccatccaggc cgctcgcacc ggcgggctcc ccgctctcga
     4021 cgccccgcag gaagtccgcc agcatcgcga cgttcatgtc ctcgcccgtg ggcgtccagg
     4081 ccacgttccc cgcctcgtgg tcgaaggtgg taagtcgctc ggcgaacccg tccacctcca
     4141 ggacgccgct cgtgcccgct attcggagcg tgaggtctcc ccatgtcgga tagcccgcgc
     4201 cgcgcgacca gctagggtct atggtggcga atatgccggg agcgattgag ccgccctcaa
     4261 gctccagcgt caggatgccg gcatcgtcta tgcccttggt cccgaagaag gtgccgacct
     4321 cggcgtagac gctgctcgcc tccgcgccga gcatccagcg cagggtatcg gcgagatgca
     4381 cggtgtgatc catgaccgcg ccgccgccgg agaggtccgg gtcgaggaac cacccgccgg
     4441 ggttctgccc gtggttcgtg ccgctgacgg ccagcacgcg cccgatggtc cccgcgcgca
     4501 ccagatcccg cgcccgacgc acggacggca ggtagcgcac cggaaacgcg atccgtagct
     4561 gcgcccccga ctcctcggag gccgcgttca tggcccgccc gtcctccacc gtcgttgaga
     4621 taggtttctc gcacagcaca tgaacgccgc tccggagcgc cggcaccacg tcccgcaggt
     4681 gatgccggtt ctcggagcag acaacgaccg cgtcaaccgc gtccaacaac tccgcgctct
     4741 cctcgaagaa ccgcaccccg aattgctccg ctgattcccg gccccgcgcc gcgtcatcgt
     4801 ccgcgatgcc gacgaactcc gcacagggga ggtcagcgag tatgctggcg tactgcggcg
     4861 cgtggacgtg ggcgaagctg acgattccga ccttcacgct catcgtgcgc ctccgggcct
     4921 gacgggttct ccggtcctga tggactcgat ggcggcggcg gagatcgcca gcgcggcgcg
     4981 ggcgtccttg ccgctcacga taggctcctc ccctccctcg atgcagcgga cgaagtgctc
     5041 cagctccgac tggtaggggc tcttgccgac cggcgcgaga ccgccgctct ccggcgcgcc
     5101 gcccgtcagg cgcagcgagg agggctcggt gctgtcggcg gtaacgaggg ccttgctacc
     5161 ggcgatctct atagagtagt tgaaggcgcc gggataaccc cagtggcctt cgaggtgcga
     5221 gatctcgcct ccctcgaaac gcagcgtgac gagggcgtag tccaggcggt tgtgctccct
     5281 tccgagcacc ccgcgcgcgt agacccgctc cacctcgccg aaacaccagc gaaggaagtc
     5341 gaagtcgtgg atcaggaggt caaccagcac cccgccgctc acccgccagt cggcgtacca
     5401 gtcgttccag cccatcaaca gcggcgaacg gcggctcgtg cgcgccaccc cgaccttgcc
     5461 gagcgagccg tcctcgacca tattctttat cctcacgtac tccgggaaga accggacgac
     5521 gtgcccgacg aagaggcgcg cgtcaccctc ctcgaaagcg cccaggatct catccgcgtc
     5581 ctccagagtg cgcgcgatgg gcttctccag gatgagatgc ttgccatccc gagcgaccgc
     5641 cagcgccagg tcccgatgga accccgtcgg caggcacacg tccacgacgt ctacctcctc
     5701 ggaccggaca agctcctcat aagtggagta agcgcgcgcc cccactagcg cggccccggc
     5761 agtggcgttc tccgggtccg tgtccgcgac gccgacgacc tcaacctccg gcatcttcgc
     5821 gtaggcggag aggtggacgg agcccatgaa cccggctccg accaccccga ccttgacgcc
     5881 ccccaacgct gcctaaccct cctctttgcc ctgtttgatg tactcttcca gcttgtcctg
     5941 gagcgtccgg gccgcctctt cgggggtctc gtcgcccttc agggcggcgt tgaagtgttt
     6001 gctcatctgg agcgacatgt ccgcgtagta gggtgagacc ggccgcggtc gggtgtgctg
     6061 tagagcgtct ttggcgaggg cggcgatggg gaccttcttc ttcacctccg cgtcgtcata
     6121 gagagccttc agggtcggga ggtacgagcc ctctatgatc ctgaacttct gttgctccgg
     6181 cgcggtcatg aactggatga actcccaggc cgcgtccggg tttttggagg ttgcgttgat
     6241 gtacagcggc tggtcgccaa ccgttccgtt gcccggttcc ccatcagcgg agggaagctc
     6301 cgagacaccg acctgctccg gcttcacctt cgactccgaa gggtcgctga tgatcgcgta
     6361 cacgtagggc cagttgcgca ggaacacggc gtcgccgttc aggaacgcgc ccgccgactc
     6421 gtcctccttg taggtcgtga cggccttcgg cgcgacgccc tcggcgatca tcgagtagta
     6481 ggtcgtgagt cccgcgattg cctccggctg cccgacgagc accctgtccg ggttctgcgg
     6541 gtccagcact tcgcccccgt gcgtccagat aaactcgcag ccgtcgcaca cgccgccctc
     6601 gtagttggct ccctggaaga cgtagccgaa cttgatcccc gagtcctgct taaccttgag
     6661 ggccatctgc ttcaactcgt cccaggtctt cggaggaccg tcgaatccgc tcttctggag
     6721 caggtccttg cggtagtaga ggagcccggt gtcggtgaac cacggcatgc cgaaggtctt
     6781 gcccttgtac gtgatcgcct cgacggagcc ggggaggtag tcgccgcgca tgtcctcggt
     6841 aaagcggtcg gagaggtccg agatccagcc attctccgcg agctgggccg tccagatgac
     6901 gtcccccagg attacgtcca ggttctcccc gccggcctgc aactgggtcc gtagcgcgtc
     6961 gaagcgctgc ccggtgtccg cgttgcccgg cctgaaactg accttgtact tgcccttgtt
     7021 ctgctcgttg aacttatcta cgagcttgct cgccgtctcc gtgtcgtccg gggaggcggt
     7081 gaagacgagc tccgtcgccc ctccgccgcc ctgctcctgc ccgccgccgc aaccggccac
     7141 gcccaggaga gccgccgccg cgagcccggc gccgccgacc ttcaggaaat cgcgtcgcga
     7201 gatggtcctc gcggaggatg ctccatcgtt cgcctccata agatgccccc tctccgccgc
     7261 tacttcagca gccactcgtg gtccgggtcg ttgtgtatcc gccactccct caccggaccg
     7321 gccatcacgt tcaaatagta cagatcgtag cccggcggcg ccgagaccac gtggtagcct
     7381 ctcggtacga gcaccacgtc cccgtcgcgg aacgccagcg tctcgtccag cgtccggtcc
     7441 tccgtataga cgcgctgcaa cgcgaagccc tgctccggct ttatccggtg gtagtaagtc
     7501 tcctcaaggt accgctcgcg tggcgggtcg tcccggtcgt gcttgtgcgg cgggtagctc
     7561 gaccagtgac cgttcggcgt tatgacctcg accacgagca ggctctcagc ggggcggtca
     7621 tccataagga tcggacggat ctcccgctcc aggttcccgt atcccctaac ctccacctcg
     7681 atgtcctccg gccttaccaa gagcggttcc gcgccgctct ccgccggagc cgagcagacc
     7741 gcaagctcca ggtccgtgac cgccccgatc cggtagccgg ttccgggtgg caggtacagc
     7801 gcgtgcggcg gaccgtcgaa gacgctcttg cgcccaccga cttcttccca ccgctcctct
     7861 ccggcgacca ctgaacactg ccccgagaga gatacgagac agatctcttc tccaagggca
     7921 tgacgctcga cgctgtggcc gctggcgagc cgcaagacct cgaagcctac gtggtcccac
     7981 ccggcggact ccggcgtgac ctttacgacg ctgccgttcg cgtccggttc gggattgctg
     8041 ctcacgatca aatcgggcaa tttaggggct cctgtctaca ggtaatggcg ctccgctttc
     8101 ctgttctcgt cgtactcctt tcgcgcggcg atgacagtat ccatctccga gacctcggcg
     8161 accggcacgt cccagaaaga ctcatagctc ggcacgccct cgtagcggtc cacggggata
     8221 tggatcacga ccgtccggtc cacactcttc gcctgcgcga gcgcttcctt tagctcgtcc
     8281 accgtcccgg tccggatcac atgcgccccc aaactctccg cattcgccgc cagatccacg
     8341 ggcagcacct cgccgggcgc cttctcggtg tcgagaccga gtgaaccgtc cttacggtag
     8401 cggtactggg tcccgaatcc ctgcgaaccg acagagcggg agagcgcgcc gatggacgcg
     8461 aacccggagt tgtccaccag cacgatggtg agcttgtaac cctcctggat ggaggtaacg
     8521 atctcggagt tcatcatcag gtaggaaccg tcgccgacca ttacataaac ttcgcgctcg
     8581 ggagcggcca tcttgatacc gaggccgccc gctatctcgt agcccatgca cgagtacccg
     8641 tactctacgt ggtagccttt cgggtcgcgc gtgcgccaga gcttgtgcag gtcccccggc
     8701 atcgaacccg ccgcgcaaac cacaacatcc tccggctccg agaacgagtt gaccgccccg
     8761 atgacctcgc cctggctcgg cagcgggcca tgatccaatg tgtagagccg ctccacctcc
     8821 agatcccacg cccggccagc ctcttcactc
//
LOCUS       DASSMB010000089         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1412816, whole genome shotgun sequence.
ACCESSION   DASSMB010000089 DASSMB010000000
VERSION     DASSMB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412816"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..74)
                     /locus_tag="VFJ72_05695"
     tRNA            complement(3..74)
                     /locus_tag="VFJ72_05695"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40..42),aa:Val,seq:tac)
     gene            complement(91..163)
                     /locus_tag="VFJ72_05700"
     tRNA            complement(91..163)
                     /locus_tag="VFJ72_05700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(127..129),aa:Glu,seq:ttc)
     gene            complement(197..268)
                     /locus_tag="VFJ72_05705"
     tRNA            complement(197..268)
                     /locus_tag="VFJ72_05705"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(234..236),aa:Lys,seq:ttt)
     gene            complement(321..791)
                     /locus_tag="VFJ72_05710"
     CDS             complement(321..791)
                     /locus_tag="VFJ72_05710"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HET7478994.1"
                     /translation="MTGRPRREYRLASVVELAERVAYYGAALFLMVTMGMVFVSAINS
                     VMEVAQTGLLSAALAILDKVLLIFIFIELLGTINTIVQEHEVVAEPFLLIGIIAVVRR
                     ILAVTVSIEQSLGTPKFDQLLFELGVLTVLVISLAISLHFTRRLERSQPNPTEQ"
     gene            complement(807..2351)
                     /gene="purH"
                     /locus_tag="VFJ72_05715"
     CDS             complement(807..2351)
                     /gene="purH"
                     /locus_tag="VFJ72_05715"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET7478995.1"
                     /translation="MKRRALVSVSDKTGVAEFARRLSEMGFEIISTGGTAKTLAQEGI
                     PVVPVSEVTGAPEILGGRVKTLHPKVHGGILADLEDPDHVTQLVDHDIAPIDLVCINL
                     YPFEETVAGGASEKEAIEQIDIGGPAMLRAAAKNFRSVTVVPSPEFYGEVLAELETDN
                     HVSEETRRRLALAAFRRTAEYDAAISAWLGGVVEGKEEKFPDRLAVRYERVAELRYGE
                     NPHQEAAYYAEEGGAGHLLSGVERLQGREISFNNLHDLDAARTLLADLTELGAPAAAV
                     IVKHANPCGAAIGTSVEDAYRKALASDPVSAFGGIVALSGEVDGDLAAEISKVFTEIL
                     VAPAFKDEAKETFSAKPNMTVLAAGPLVRPPLAAKHVTGGILLQDADTMEDDAGFRVV
                     TKADATEAQMEDLRFAWTVARSVKSNAIVLVKDGATVGVGAGQMSRVDSSGIAVKKAG
                     DRVHGAVAASDAFFPFADGVEALAESGVAAVIQPGGSKRDEEVIEAADRYGVAMIFTG
                     RRHFLH"
     gene            complement(2348..>2668)
                     /locus_tag="VFJ72_05720"
     CDS             complement(2348..>2668)
                     /locus_tag="VFJ72_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HET7478996.1"
                     /translation="GAGYMRVLSPVFLERFPAVLNVHPSLLPEFRGLHAIERTLEAGV
                     TETGVTVHFMVEEVDAGPVVAQERVPVLPGDTKESLLERLHPVEHRLLVRAVADYFLG
                     RVSV"
BASE COUNT          458 a          946 c          805 g          459 t
ORIGIN      
        1 agtgggcgat gctgggttcg aaccagcgac cccctccttg taagggaggt gctctaccgc
       61 tgagctaatc gcccggaata cgccggaacg tgcccccaac gggattcgaa cccgtgccgc
      121 cgccttgaaa gggcggtgtc ctgggcctct agacgatggg ggcagagccc tgttcgggcg
      181 caagccttcc ggctcgtgag ccaggtaggg atcgaaccta cgacacgagg attaaaagtc
      241 ccctgctcta ccactgagct actggcccgc ctagcatggg agtttaccaa acacgtccgg
      301 gctttggagc gtatggcggt ctactgctca gtcgggttcg gctgggatct ttcgaggcgg
      361 cgggtgaagt gcagggagat cgccagtgag atcacgagca ccgtgagcac gccgagctcg
      421 aagaggagtt ggtcgaactt cggcgtgccg aggctctgct ctatggagac ggtcacggcc
      481 aggatgcggc gcacgacggc tatgatcccg atgagcagga acggctcggc gacgacctcg
      541 tgctcctgga cgatggtgtt gatggtgccg agaagctcga tgaagatgaa gatcaaaagt
      601 accttgtcca gtatcgccag ggccgccgag agaagcccgg tctgcgcgac ctccatcacg
      661 ctgttgatcg cagagacgaa gaccatcccc atcgtcacca tcaaaaagag cgccgcgccg
      721 tagtacgcca ccctctcggc gagctcgacc acgctcgcga gccggtactc ccttctcggc
      781 cttccggtca cgccggacct cccatatcag tgcaggaagt gccggcgtcc agtgaagatc
      841 attgccacgc cgtaccggtc cgccgcctca atgacctcct catcgcgctt ggagccgccg
      901 ggctgtataa cagccgcgac tcccgactcc gccagggcct ccacgccgtc ggcgaatggg
      961 aagaaggcgt cgctggccgc gaccgcgccg tggacccggt ctccggcttt ctttaccgcg
     1021 atcccggagg agtccacccg gctcatctga cccgccccga caccgaccgt ggccccatcc
     1081 ttgacgagca cgatggcgtt gctcttgacg ctccgggcta ccgtccaggc gaagcgcagg
     1141 tcctccatct gagcctccgt ggcatcggcc ttcgtcacca cccggaatcc ggcgtcatcc
     1201 tccatcgtgt cagcgtcctg gagaaggatg cctccagtga cgtgcttggc ggcgagcggc
     1261 gggcggacga gcggaccagc ggcgagcacg gtcatgttcg gcttcgcgga gaaggtctcc
     1321 ttcgcttcgt ccttgaaggc gggggctacg agtatctcgg tgaagacttt ggagatctcc
     1381 gccgccaagt caccatccac ctcgccgctc agggcgacga tgccgccgaa cgcggagact
     1441 gggtccgagg cgagcgcctt gcggtaggcg tcttccaccg acgtaccgat agcggcgcca
     1501 caggggttcg cgtgcttgac gatgacggcg gcggccggag ccccgagttc cgtcaggtcg
     1561 gcgaggagcg tccgggcggc gtcgagatcg tggaggttgt tgaaggagat ctcccgcccc
     1621 tgcaacctct ccaccccgga gagcagatgc cccgctcccc cctcctccgc gtagtacgcc
     1681 gcctcctggt gcgggttctc gccgtagcgc agttccgcga cccgctcgta gcgcaccgcg
     1741 agccggtccg ggaacttctc ctcctttcct tccaccacgc caccgagcca ggccgagatc
     1801 gccgcgtcgt actccgccgt ccgccggaac gccgcgaggg ccagcctacg ccgcgtctcc
     1861 tccgagacgt ggttgtccgt ctcaagctcc gcgaggacct ccccgtagaa ctccggcgac
     1921 gggacgaccg taacgctccg gaagttcttc gccgccgcgc gcagcatcgc cggtccgccg
     1981 atgtctatct gctcgatggc ctccttctcc gacgcgccgc ccgcgacggt ctcctcgaac
     2041 ggatagaggt ttatgcacac caggtcaatc ggggcgatgt cgtggtccac gagctgagtt
     2101 acgtggtccg ggtcctcaag gtcagcgagg atgccgccgt ggaccttcgg gtgcagcgtc
     2161 ttgaccctgc cacccagtat ctccggcgct cccgttactt cggataccgg cacgaccggg
     2221 ataccctctt gcgcgagcgt cttagcggtg ccgccggtcg agatgatctc gaaccccatc
     2281 tccgagagcc gccgcgcgaa ctccgccacc cccgtcttgt ccgaaaccga caccagcgcc
     2341 cgccgcttca taccgacacc ctccccagga agtagtccgc taccgcccgg accagcaacc
     2401 ggtgctccac cggatgcagc cgctccagca ggctctcctt cgtatctccc ggcaggaccg
     2461 gcaccctctc ctgcgccacc accggtcccg cgtctacctc ttcgaccatg aaatgcaccg
     2521 taacccccgt ctctgtaact cccgcttcaa gcgtccgctc tatggcgtgc aaaccccgga
     2581 actccggcag gagcgatgga tgcacgttca gcaccgccgg aaaccgctcc aggaagaccg
     2641 gcgagagcac ccgcatataa cccgcccc
//
LOCUS       DASSMB010000090        15943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_399093, whole genome shotgun sequence.
ACCESSION   DASSMB010000090 DASSMB010000000
VERSION     DASSMB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15943
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399093"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..668)
                     /locus_tag="VFJ72_05725"
     CDS             complement(267..668)
                     /locus_tag="VFJ72_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7478997.1"
                     /translation="MAGPETPKLMVDVVIPSEGEIVLIRRGSEPFEGQWALPGGFVEV
                     GETVEQAAVREAAEETGLAVEIAGLVGVYSEPERDPRGHNVSVAFLARVLSGEMQAAT
                     DASEVEVLDPDEVDLAFDHAKIIADARDLDS"
     gene            936..2411
                     /locus_tag="VFJ72_05730"
     CDS             936..2411
                     /locus_tag="VFJ72_05730"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET7478998.1"
                     /translation="MQVLCVAVVLLVPQVAWASSYIAGPAVQVSGTSPFTPGCGTANQ
                     TGTNFVNSEIEPFVDVNPANGDNIVGSWQQDRWSDGGSRGLVAGVSRDNGQSWTLSPV
                     RGTSQCTGNPLYERASDPWLSFSPNGSLHHVSLSFNMTNNDNAVIVNKSKDGGLTWSA
                     PKTIIRDTDFTVFNDKESITADTNNSKFVYVIWDRLVAPRGERANIRASQHAAAFAGP
                     IWFSRSTDGGNTYEKARQIFDPGTLQQTIGNQIVTRPQGDLVDIFNLIQNTRDLQMRG
                     FNVSVIRSTDHGATWSNPIRFDTMVNPGVTDPDDGDAVRTGDIIPDIAVDPRNGNLYA
                     VWQDKRFDDGQHDSIALSSSTDGGRTWSPAVKVNKTPTNIPTGNQQAFTPAVDVASDG
                     SVAVTYYDFRNNTAAPDTLPTDYFVVHCDPTSDCSRAGNYGDEIRLTDTSFDMEKAPN
                     ARGYFIGDYEGLANDGTDFTPFFSQSHGTDPASIFFRRVGP"
     gene            2526..4019
                     /locus_tag="VFJ72_05735"
     CDS             2526..4019
                     /locus_tag="VFJ72_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET7478999.1"
                     /translation="MKTPSQRNPSAQPDVPLSAGLGLAAFGLYLLTLAPTVLYYSPKN
                     YDSAHLQVVAYVLGIPSFTGYPTYVMLAHLFTYLPVGDPAYRVNLASAVFAALAVAVI
                     YLLCRRLGAGGFASAAGALAFGFGATFWSQAVIAEVYTLHVLLMALFLLTLLVWRARR
                     REPRSDRYLLLASLLGGLAMTNHLTSVFLLPAAVIFMAVVDRRRLLDPALLLKSAGFF
                     ALGLLPYLYLPVRASMDPPLVNAGPADNPSTLSGFLDLVSGGDFKGRMFVFGPGELPR
                     RMSIYGEHLLDNLNPALLALAAVGLAYLALRDRAALILLGTFFVLNLVYALEYDIEDL
                     EIYFVPTYLILCLTVSLGIQALLTWARERRARRVLAPAIILLVLAAVLVYVPGTYRTV
                     DRSGDYRGREIVETVARGTQPGSAVLYHGRSLHYMQLVEGRRRDIRLEDPFYTSDWVQ
                     RARRDLRYGPVYVLYPGATNTRLFRKAGYELEPIREGMLYKVTKRST"
     gene            4016..4627
                     /locus_tag="VFJ72_05740"
     CDS             4016..4627
                     /locus_tag="VFJ72_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7479000.1"
                     /translation="MKVLCISDRVESMLHGPSLNHYAAGVDAVISCGDLPFSYLDYIV
                     TFLGAPVYYVLGNHDPAPDSKDYPEGCIPLDGEVVDIGGVFVAGLSGSRLYSGGPNQY
                     TERQMNRRAWALSMKIRWRKLLGRPAPSVFVSHSPAFGLGDREDLPHVGFRSFLGLMD
                     RFEPDLWLHGHVHLYGPDQEKQRIEKHGKTKLVNVYGHKFIEI"
     gene            4685..5581
                     /locus_tag="VFJ72_05745"
     CDS             4685..5581
                     /locus_tag="VFJ72_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479001.1"
                     /translation="MVDFMDPNEQADRDFTRARRRAFLRRVGAYLRNDPGSNQLLSFE
                     EVKSALGAVEQVYVGMREVPVEKVVGSVGRHRDFDRAFLPSKGDLGSRWKKIDQMMQR
                     AEELPPVSLFKIGDAYFVQDGNHRISVARLRGVEMIDAEVTELRSRVPIDSALTARDL
                     LHKLEQRRLLERLPLDRAVPEIKIQFSDVTDYRRFATYVEAHGYRLSQLWRRYVSHEE
                     ALRDWYEYSYTPIADMIREERILAAFPGRTELDLYFWILNHREQLALESRDDRVSPEA
                     AKDDILLKEGRRRKNTLPGTYT"
     gene            5682..6731
                     /gene="sppA"
                     /locus_tag="VFJ72_05750"
     CDS             5682..6731
                     /gene="sppA"
                     /locus_tag="VFJ72_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HET7479002.1"
                     /translation="MSQENGPARDLTNETPQGAPSNRRRRWPWVVGGVAALGLLALIA
                     GAVGLALALGGGGYSSPPTYEEEYVSGDGSDRIAVVPVEGEISSADSTLGGTQPVTTP
                     EGLEDALRQAGDDDSVAAVILEVNSPGGGVTASDEMHQAILDFKEKTDKPVIVSMDDV
                     AASGGYYISTAADEIVANRTTLTGSLGVILQLNDFSEAADKYGIKQQVIKSGKYKDIG
                     NAFRKIKPEEREILQKIVDENYDQFVSVIVKGRGLPEKKVREIADGRIYSGIQAKKLG
                     LVDEFGGLDEAAKVARKMANIDKATVVRYTQPPSLTDTLLATFGPQKPEAVQVMEAAG
                     IDLTPEIKYLYRPGL"
     gene            complement(6745..7665)
                     /locus_tag="VFJ72_05755"
     CDS             complement(6745..7665)
                     /locus_tag="VFJ72_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET7479003.1"
                     /translation="MRRPVSISFTVLLAVGIAFSVALYAVSEAQAATYSQVVDNTTAG
                     RFLASSKWISSNYSAQRYGTNYRVLKKPGSTSISAKYKIKTPSKGSYRVLARWPANSG
                     YNNRTRFLVKSSGGWKSKVVNQRQNGGQWVSLGTYTLDAGDSNRVMVSSKSSGTGYII
                     ADAVKVVKSTTTSTTSSSAVTGSDIVAEARRHLGEPYVFGAAGPSSFDCSGLTLYVYK
                     KVAGISLPHSAASQYNYGTSVSRAELKPGDLIFGHANGGSGIEHVGIYAGKNSSGDNL
                     MIHAGNPQTDVQLTTYESWYTVVGYKRLIR"
     gene            complement(7875..9383)
                     /locus_tag="VFJ72_05760"
     CDS             complement(7875..9383)
                     /locus_tag="VFJ72_05760"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET7479004.1"
                     /translation="MQVENRNFEVRNLIGGSWEEKDGRETEPVYDPATGQVIAETPLS
                     TREDVDRAVARAKEAFPGWAGTPVGERARILFRFKMLLEDHFEELRDLVTLENGKDAK
                     DAGGEVRRGIEVVEFACGMPTLMMGETVRDVARGIDNISYRFPLGVVAAITPFNFPCM
                     VPLWTLPVAIGAGNTYILKPSERTPLSAQRLGELLTEAGLPDGVFNIVNGAREAVDSI
                     LEHPGIEAVSFVGSKPVAEHVYKHGAAHGKRVQALAGAKNSMIVMPDAVLDKAVPNIV
                     SSSYGNAGERCLAGSVLVAVGDVADELVERVKEAASAMKVGPGSKEGSELTPVIRDSH
                     REKVRSYIDTGEEEGAEVVLDGRTPPLEDGFFMGPTLLDNVTGDMRVGREEIFGPVLS
                     VVRVGSLDEAIEFTNGSPFGNACSIFTENGAAVRHWRENIEAGMLGVNIGVAAPMAFF
                     PFNGIKDSFYGDLHATGKDGVRFFTENKVETVRYFSEPGVTEAVKRPIESGA"
     gene            complement(9510..10052)
                     /locus_tag="VFJ72_05765"
     CDS             complement(9510..10052)
                     /locus_tag="VFJ72_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479005.1"
                     /translation="MSERQQPEAANTLAQAFGESYQTVMKSAVDAQQRNVKLAQGWAE
                     AVTGVVESQTETNRALTRAMESFVKLADETVKSQERTSRALAESLDAYRDVLEKAVSM
                     QERNENLVRNFFGGAPSELQRGVEESQTVANNIMDGSQKQMEAFQKMMSEAMDSYTNL
                     MNAPFALYQKNLEAFGKRGG"
     gene            10150..11106
                     /locus_tag="VFJ72_05770"
     CDS             10150..11106
                     /locus_tag="VFJ72_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7479006.1"
                     /translation="MRAMVTPEFGGPDLFEEQDLQRPEAGPGEVVVRVVVAGTNPVDA
                     KLRAGDIPGAVETPVVLGADVSGVVEEVGAGVTDLKPGDEVYYSPELFGPVSNGSYAE
                     FNLVPAAIVAPKPSSISHEEAAAVPLAGGTAWEAIVRRLGVVPGETVLIHGGAGGVGS
                     FAVQIARAAGARVIATAGSSNQETLRELGADVCLDYSSDDVAGTASAENGGEGVDAVF
                     DTVGGQVEEATGYTRPFGRIATILPPQGDLTQLYLKNQTLYGVFLTRERRRLVELARL
                     MDRGQVKPLVDEVLDLSEVGKAHERLESGHGRGKVVLRVSRG"
     gene            11225..12529
                     /gene="egtB"
                     /locus_tag="VFJ72_05775"
     CDS             11225..12529
                     /gene="egtB"
                     /locus_tag="VFJ72_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET7479007.1"
                     /translation="MDTKRGESKEALAGKLEEARERTRKLLAPVSDQDLARQHDRIMS
                     PLIWDYGHIGNYEELWLMERAFGHGLSDRELYDMYDASLHPREERPSLNLLDRSDADV
                     YLDAVRKAVLESLEAADLKGEDPLLHDGFVYNMILQHEAQHNETMLQTLQLMRGDGYH
                     PEPEIDLPENPGVEDEMVRIPAGPFVMGTDDRSLALDNERDAHEVDVPEFYLDRMPVT
                     NREYLEFVEDGGYEREELWNSAGWKWVQSETIAAPKHWYQRDPHSWWTKCFGFDEPLR
                     PDAPVVHVSWYEADAYARWTGKRLPTEAEWEKAASWDPESGTGRLFPWGDEPPTPEKA
                     NLDQLAFRTSDVGAYPAGASAYGILDMIGNVWEWTASDFAPYPSFQSFPYREYSEVFF
                     GPDYKVLRGGSWATRPVAVRNTFRNWDYPMRRQLFVGFRCAR"
     gene            12590..13363
                     /gene="egtC"
                     /locus_tag="VFJ72_05780"
     CDS             12590..13363
                     /gene="egtC"
                     /locus_tag="VFJ72_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HET7479008.1"
                     /translation="MAAYLGGPETSLSSVVLKPEHSLLVQSYAPREMMSGVVNADGFG
                     VGWYVPGDDEPAVYRSNSSIWSDRSFAGIAPKIRSQRIFAAVRSATPGLPVEESGVPP
                     FDSGRFMFMHNGAIQDFRRRAMRPLRDSLSDESHAGLLGVSDSETIFAGLLDRLWDGG
                     DIAGALAATIRHVSGVCAVAGVKASLNLALTDGEAMAFTRHSTEGPGNSLYFAERMEA
                     LPGAVVVASERLDGDAGWREVPDRHLLHVTEGSVTLRAL"
     gene            13377..14411
                     /gene="egtD"
                     /locus_tag="VFJ72_05785"
     CDS             13377..14411
                     /gene="egtD"
                     /locus_tag="VFJ72_05785"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET7479009.1"
                     /translation="MNRGTSRGTGNINITLLDEVGSGEDQISRDVRAGLLSSPKDLSP
                     WPKYFYDAEGSRLFEEITELPEYYQTRAELSILQDRAKEIIEITGSRELVELGSGSAS
                     KTRALLDALLGSRDETVSETPRYMPLDVSESALRESGERLTVEYPQLEIQGYVGDFDT
                     SLGRLLGPESANGSGGRLVIFLGGTIGNFTPEKRRSFLRKIRAGLTPGEHALIGVDLV
                     KDRETLEAAYDDSARVTARFNKNMLSVLNNRLGAGFEPDLFEHRAIYNEQEQRIEMWL
                     DSCVDQNVPIPALGLDVRFAAGEGMRTEISTKFTPDSASEMLEEAGLELLDLYTDERN
                     LFGLALGRAG"
     gene            complement(14416..14574)
                     /locus_tag="VFJ72_05790"
     CDS             complement(14416..14574)
                     /locus_tag="VFJ72_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HET7479010.1"
                     /translation="MDILRIIVAILLPPLGVFLQVGIGKHFWINVILTILGYIPGIIH
                     AVWVIAKY"
     gene            14777..15298
                     /locus_tag="VFJ72_05795"
     CDS             14777..15298
                     /locus_tag="VFJ72_05795"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7479011.1"
                     /translation="MDLRSVQKPLKDRYRNEPGSSKITLKATGSETDAPISCSVDIGR
                     AVYEAEAHTGVGGMGTAACSGDLLLGALAACAQITCQMVASAMNVPTNSIEATAEGDL
                     DLAGTLGISKDAQVGFEEIRLTLKVDAPEATSEQLDALHEKTEQYCVVMQTLAHPPRL
                     RTEWVRENESEAG"
     gene            complement(15302..15646)
                     /locus_tag="VFJ72_05800"
     CDS             complement(15302..15646)
                     /locus_tag="VFJ72_05800"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7479012.1"
                     /translation="MTEDKTIKKVSSKTSPKGGMGQKYLADGKSVSMRLWDGEEPGDS
                     KPEARRDYETVGYVIDGRAELHLEGQKVLLEPGDSWLVPKGASHTYNILETFTAVEAT
                     SPPAEVHGRDEG"
     gene            complement(15732..>15943)
                     /locus_tag="VFJ72_05805"
     CDS             complement(15732..>15943)
                     /locus_tag="VFJ72_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479013.1"
                     /translation="LGVMPASAPGSPPLPPGRLRRMMRSEWTYVTLYVLYMLVLSLIF
                     AGLTARVLENAATVYRAFEQLGITL"
BASE COUNT         2964 a         5229 c         4922 g         2828 t
ORIGIN      
        1 gttcagcaca aacatagtac tcgcctgcaa ctcctatcat gttattagaa atacgattca
       61 ccatatagat tcattccctt cctctgtctg acaccatatt cgcaaactaa agtatgacta
      121 tatctaaata aagaggaaaa ccatacaatc cacaccattg cgtgtagttc tcgactgcga
      181 taccgccgat gcaggagacg accagtcctc cgtcgatatt ccaccgggca ccgctctact
      241 gtcgtacgcc gctcagggcg ggcgactcaa gagtccaggt cgcgggcgtc ggcgatgatc
      301 ttcgcgtggt cgaaggcaag atctacctcg tccgggtcca ggacctcgac ctccgaggcg
      361 tcggtagccg cctgcatctc tccgctcagg acgcgggcga gaaaggcgac gcttacgttg
      421 tggccgcggg gatcgcgctc cggctccgag tagacgccga cgagacccgc gatctcgacc
      481 gcgaggcccg tctcctcagc ggcctcgcgc accgccgcct gctcgaccgt ctctcctacc
      541 tccacgaagc ctcctggcaa agcccattgt ccctcgaaag gctcgctgcc ccgccgaatc
      601 aggactatct ctccctccga agggatcacc acgtcaacca tcagcttcgg ggtctccggt
      661 cctgccatct ttgcgcctcc cgctcgaata caccgatagc gtactacccg gagcgcagat
      721 ggagataaca gaccctacct tcctataacc ctgcataaag ctacaccgaa gataatcttt
      781 cgagaccagg ctcctgcgac ttgcgccgca tttagcaaca tgtatatcat ttcgttcagg
      841 gactgcttcc gggcagacgt gacttcgacc cggtaccgga gccagaccgg gttctctcaa
      901 gggggtgcgc cttggatagg aaacggcgac gactcgtgca ggttctgtgc gtcgcggtgg
      961 tgttgctggt gccgcaggtc gcgtgggcga gctcgtacat cgccggtccc gcggtacagg
     1021 tatccgggac gagcccgttc acgccgggat gcggtaccgc gaaccagacg gggaccaact
     1081 tcgtgaacag cgagatcgaa ccgttcgtgg acgtgaaccc ggcgaacggc gacaacatcg
     1141 tgggatcgtg gcagcaggac cgctggagcg acggcgggtc cagaggtctg gtcgcgggcg
     1201 tgagccgcga caacgggcag agctggacgc tctcgccggt caggggcacc agccagtgca
     1261 cgggcaaccc gctctacgag cgcgcgtccg acccgtggct gtcgttctct cccaacgggt
     1321 ccctccacca cgtcagcctc tcgttcaaca tgaccaacaa cgacaacgcc gtgatcgtca
     1381 acaagtccaa ggacggcggg cttacgtgga gcgcgcccaa gaccatcatc cgcgacaccg
     1441 acttcaccgt cttcaacgac aaggagtcca tcaccgcgga caccaacaac tcgaagttcg
     1501 tgtacgtgat ctgggaccgc ctcgtggctc ccaggggtga gagagcgaat atccgagcat
     1561 cgcagcacgc ggcggcgttc gcgggtccga tctggttctc ccgcagcacg gacggcggca
     1621 acacctacga gaaggcgcgc cagatcttcg accccggaac cctgcagcag accatcggca
     1681 accagatcgt caccaggccg cagggggatc tggtggatat cttcaacctg atccagaaca
     1741 cccgcgacct gcagatgcgc ggctttaacg tgagcgtcat ccgctccacc gaccacggcg
     1801 cgacgtggtc gaatccgatc cggttcgaca caatggtcaa ccccggcgtg accgaccccg
     1861 acgacggcga cgcggtccgc accggagaca tcatcccgga catcgcggta gacccgagaa
     1921 acggcaacct ctacgccgtg tggcaggaca agcgcttcga cgacggccag cacgactcca
     1981 tcgccctctc ctcctcgacc gacgggggcc gcacgtggtc accagccgtc aaggtcaaca
     2041 agacgcccac gaacatcccg accggcaacc agcaggcgtt caccccggcg gtggacgtcg
     2101 cctccgacgg gagcgtcgcg gtcacctact acgacttccg caacaacacc gcggccccgg
     2161 acaccctgcc gaccgactac ttcgtcgtgc attgcgaccc gacgagcgac tgctccaggg
     2221 cggggaacta cggcgatgag atccgtctga ccgacacctc cttcgatatg gagaaggctc
     2281 cgaacgcccg cggatacttc atcggcgact acgagggtct cgcgaacgac ggcacggact
     2341 tcacgccgtt cttctcgcag tctcacggaa cggacccggc gagcatcttc ttcaggagag
     2401 tcggcccgta agcggagcca aagcacgtga gaagaggccc ggagatctcc gggcctctct
     2461 tgtttatgtc ctctctccga gtcaccccgg aatgatggtc tccagcctca ggtaagatcc
     2521 cggacgtgaa aaccccttcc cagaggaacc cgtccgccca gcccgacgtc ccgctctccg
     2581 ccggtctcgg gctggcggcc tttggcctct acctgctcac gctcgcgccg accgtcctct
     2641 actactcccc gaaaaactac gactcggcgc acctccaggt cgtcgcctac gtgctcggca
     2701 tcccgagctt caccggctac ccgacatacg tgatgctggc gcacctgttt acatatttgc
     2761 cggtcgggga cccggcctac cgcgtcaacc tcgcctccgc cgtcttcgcc gcgctggcgg
     2821 tcgcggtgat ctacctgctg tgccggaggc tcggggcggg aggattcgcg tcggcggcgg
     2881 gcgccctggc gttcgggttc ggcgccacgt tctggagcca ggccgtaatc gccgaggtct
     2941 acaccctgca cgtcctcctg atggctctct tcctgctcac gctcctggtc tggcgggctc
     3001 gccgccgcga accgcgaagc gaccgctacc tgttgctcgc ctctctcctc ggcggactgg
     3061 cgatgaccaa ccacctgacg agcgtcttcc tgctgccggc ggcggtgatc ttcatggccg
     3121 tcgtggaccg gcgcaggctg ctggaccctg ccctgctgct aaagagcgcc ggcttcttcg
     3181 cgctgggtct gctgccgtac ctctacctgc cggtacgggc ctcgatggac cctccgctcg
     3241 tgaacgcggg accggcggat aacccgtcca cgctatccgg attcctggac ctcgtctccg
     3301 gcggagactt caagggccgg atgttcgtgt tcggtcccgg cgagcttccg agacggatgt
     3361 cgatctacgg tgagcacctc ctagacaacc tgaacccagc gctgctggcg ctggccgcgg
     3421 tcgggctcgc gtatctggcg ctcagggacc gggccgcgct catacttctt gggacgttct
     3481 tcgtcctcaa cctcgtctac gcgctggagt acgacatcga ggacctggag atctacttcg
     3541 tccccacgta cctgatcctc tgcctgaccg tctctctcgg aatacaggca ctcttgacgt
     3601 gggcgcggga gcggcgtgct cgacgggtcc tcgcgcccgc gatcattctc ctggtgctgg
     3661 cggcagtcct cgtctatgtg ccggggactt acaggacggt ggaccggagc ggagactaca
     3721 gggggcgcga gatcgtggag accgtggcgc ggggcacaca gcccggctcg gcggtcctct
     3781 accacggacg ttcgctccac tacatgcagc tcgtggaggg ccggaggaga gacatcaggt
     3841 tggaagatcc cttctacacc agcgattggg tgcagagggc gaggcgggac ctccggtacg
     3901 gaccggtcta cgtgctgtat ccgggcgcga ccaacacccg tctcttccgg aaggccggat
     3961 acgagcttga gccgatcaga gaaggtatgc tttacaaagt gacgaagaga tcaacatgaa
     4021 ggtcctctgc atcagcgacc gggttgagtc catgctgcac ggcccgagcc tgaaccacta
     4081 cgccgccggg gtggatgctg tcatctcgtg cggcgacctg ccgttcagct acctggatta
     4141 catcgtcacg ttcctgggcg cgccggtcta ctacgtcctc ggaaaccacg accccgcgcc
     4201 ggacagcaag gactacccag agggctgcat accgctcgac ggcgaggtgg tggatatcgg
     4261 gggcgtgttt gtcgcggggc tctccggctc gcggctctac tccggcggcc cgaaccagta
     4321 caccgagcgg cagatgaacc gccgggcgtg ggcgctctcg atgaagatcc gttggcgcaa
     4381 gctcctgggg cgtccggcgc cgagcgtctt cgtcagccac tcaccggcct ttggtcttgg
     4441 agaccgggaa gacctgcccc acgtcgggtt ccgctcgttc ctcggcctca tggaccgctt
     4501 cgagccggac ctctggctgc acgggcacgt ccacctgtac ggcccggacc aggagaagca
     4561 gcggatagag aagcatggaa agacgaagct cgtaaacgtc tacgggcaca agttcatcga
     4621 gatctaaggc ggtgttttcg ctccaaacct tgttgtgcgg gacatgcgga tgtagattga
     4681 gcaagtggta gatttcatgg accctaacga gcaggccgac agggacttca cccgcgcccg
     4741 ccgaagggcg tttctgagac gggtcggcgc ctacctgcgt aacgaccccg gctccaacca
     4801 gctcctctca ttcgaggagg taaagagcgc cctcggggca gtcgaacagg tctacgtggg
     4861 gatgcgcgaa gtgcctgtcg agaaggtcgt cggcagcgtg ggacggcacc gggacttcga
     4921 ccgggcgttc ctgccgtcga agggagacct cgggtcgcgc tggaagaaga tcgaccagat
     4981 gatgcagcgg gccgaggagc ttccgccggt gagcctgttc aagatcgggg acgcctactt
     5041 cgtccaggac ggcaaccacc gcatctccgt cgcccggctg cggggggtcg agatgatcga
     5101 cgccgaggtc accgagcttc gcagccgcgt gcccatagac tcggcgctga ccgcccgcga
     5161 tctgctgcac aagctggagc agcggcgcct tttagaacgt ctcccgctgg accgggccgt
     5221 cccggagatc aaaatccagt tctcggacgt gacggattac cggcgcttcg ctacctacgt
     5281 cgaggcccac ggctaccggc tctcgcaact ctggaggcgc tacgtctcac acgaagaggc
     5341 cttgagggac tggtacgaat actcctatac tccaatagcg gacatgatcc gggaggagcg
     5401 catcctcgcc gccttccccg gtcgcaccga gctggacctg tacttctgga tcctcaacca
     5461 ccgcgagcaa ctcgcccttg agtcccgcga cgaccgcgtc tcccccgagg ccgccaagga
     5521 cgacatcctc ctgaaggaag gccgccggcg caagaacact ctccccggca catacacctg
     5581 agatcccgcc catcctcccg ccccgtctcg cgctcccgga taccctccgt ataatctaac
     5641 ctgcaacagt tcttgagtac tacatcggga aggaacagag cttgagccag gagaacggac
     5701 cggcgcggga cctgacgaat gagacaccgc agggcgcgcc ctcgaaccgg cggcgcaggt
     5761 ggccgtgggt cgtgggcgga gtggccgccc tcgggctgct ggcgcttatc gccggggcgg
     5821 tagggctggc tctcgctctc ggcggtggcg gctacagctc gccgcccacc tacgaagagg
     5881 agtacgtctc gggcgacgga tcggacagga tagcggtcgt gccggtggaa ggtgagatct
     5941 cctccgccga cagcacgctc ggcggtacgc agccggtcac gacgccggag ggtctcgaag
     6001 acgcgctccg ccaggccgga gacgacgaca gtgtcgccgc cgtgatcctg gaggtcaact
     6061 cccccggcgg cggcgtcacg gcgagcgacg agatgcacca ggccatcctc gacttcaagg
     6121 agaagacgga caagccggta atcgtctcga tggacgacgt cgcggcctcc ggcggctact
     6181 acatctccac cgccgccgac gagatcgtcg ccaacaggac caccctcacc ggctctctcg
     6241 gcgtcatact ccagctaaac gacttctcgg aagccgccga caagtacggc atcaagcagc
     6301 aggtcataaa gagcggcaag tacaaggaca tcggcaacgc tttccgcaag ataaagcccg
     6361 aggagcgtga gatccttcag aagatagtag acgagaacta cgaccagttc gtctcggtca
     6421 tcgtgaaggg ccgaggtctc cccgagaaga aggtccgcga gatcgccgac ggacgcatct
     6481 actccggtat acaggcgaag aagctcggcc tcgtggacga gttcggcggg ctggacgagg
     6541 cggcgaaggt cgcgcgcaag atggctaata tagacaaagc gaccgtcgtc cgctacacgc
     6601 agccgccctc cctaaccgat acgcttctcg ccacattcgg cccgcaaaaa ccggaggccg
     6661 ttcaggtaat ggaggccgcg gggatagacc ttacgccgga gatcaagtac ctgtaccgtc
     6721 cggggttgta gatcgggggt ttctctaccg gatgagacgc ttatagccga ccacggtgta
     6781 ccagctctcg taggtcgtga gctgcacatc ggtctgaggg ttgccggcgt ggatcatcag
     6841 gttatctccg gagctatttt tcccggcata gattcccacg tgctcgatcc cactcccgcc
     6901 gttcgcgtgg ccgaagatca aatctcccgg ctttagctcg gccctggaga ccgaggtacc
     6961 gtagttgtac tgcgacgcgg cgctgtgcgg cagagagatt cccgctacct tcttgtagac
     7021 gtacaaggtg agcccggaac agtcgaaaga actgggcccc gcagctccga acacataagg
     7081 ttcaccgaga tgcctccgcg cctccgcgac gatgtccgac ccagtaaccg cgctcgaact
     7141 cgtcgtgctg gtcgtcgttg acttcacgac ctttacggcg tcggcaatta tgtacccggt
     7201 accggaactc ttgctggaga ccataacgcg gttggagtcg ccggcgtcga gcgtgtaggt
     7261 acccagcgaa acccactggc cgccattctg acgctggttg acgaccttgc tcttccagcc
     7321 acccgaactc ttgaccagga accgcgtgcg gttgttgtag ccggagttgg ccggccagcg
     7381 ggcaagcacc cggtaagagc ccttggacgg cgtcttgatc ttgtacttgg cgctgatcga
     7441 ggtcgaaccc ggcttcttga ggaccctgta gttggtccca taccgctgag cgctgtagtt
     7501 gctggagatc cacttgctcg aagccaagaa acgccccgcc gtggtgttgt ccacgacctg
     7561 agagtaagtc gcggcctgtg cctcggatac cgcgtaaagc gccacggaga atgcgatgcc
     7621 caccgcaagc aatacggtaa atgagatcga cactggtctc cgcaacgggt cccccctcca
     7681 gaactcgtaa tcaacttcga cgcattgtaa cccggagtgt tatgaaaacg ctacggtttc
     7741 taaagaaaat tttacgaagt tgtaaagaaa ttgtttcggt cgctgtccat aaccaacctt
     7801 cgcggcggga agaaaagcgg cggacacctt cagaaaatgc ccgccgcctc gatactggga
     7861 cagactcctg ggcgctaggc gccgctctcg atgggccgct tcacggcttc ggtgacgccc
     7921 ggctcggaga agtagcgtac cgtctccacc ttgttctcgg tgaagaaccg gacgccgtcc
     7981 ttaccggtgg cgtgcaggtc gccgtagaag gagtctttta tgccgttgaa cgggaagaac
     8041 gccatcggag cggccacgcc tatgttcacg cccagcatcc cggcctctat attctcccgc
     8101 cagtgccgga ccgccgcgcc gttctcggta aagatggagc aggcgttccc gaacgggcta
     8161 ccgttggtga actctatggc ctcgtccagc gagccgacgc gcacgacgga gaggacggga
     8221 ccgaagatct cctcccgccc gacccgcatg tcgccggtta cattgtccag gagcgtcggc
     8281 cccatgaaga aaccgtcctc aagcggcgga gtcctgccgt cgagcaccac ctcggcgccc
     8341 tcttcctcgc cggtgtctat atagctcctg accttctcgc ggtgcgagtc acggatgacg
     8401 ggcgtgagct ccgagccctc tttactgccc ggccccacct tcatcgccga cgcggcttcc
     8461 ttgaccctct cgacgagttc gtcggccacg tccccgaccg cgacgaggac gctgccggcg
     8521 agacaccgct ccccggcgtt gccgtaggag gaggagacta tgttgggaac tgccttatcc
     8581 aggacggcgt cgggcatgac gatcatggag ttcttcgctc cggccaacgc ctgtacccgc
     8641 ttgccgtgag ccgccccgtg cttgtagacg tgctcggcga cgggctttga gcccacgaac
     8701 gagacagcct ctattccggg atgctccagg atggaatcta cggcctcccg cgccccgttc
     8761 actatgttga agaccccgtc ggggagtccg gcttccgtga gaagctcacc caggcgctgc
     8821 gccgagagag gcgtcctctc ggagggcttg aggatgtagg tgttgcccgc ccctatggcg
     8881 acgggcagcg tccacagcgg aaccatgcac gggaagttga acggcgttat ggccgccacc
     8941 accccaagcg ggaagcggta ggagatgttg tctatcccgc gcgccacgtc gcgcaccgtc
     9001 tcgcccatca tcaaggtcgg catgccgcag gcgaactcga cgacctctat gccgcgccgc
     9061 acctcgccgc cggcgtcctt cgcgtccttg ccgttctcca gggtgacgag gtcccgcaac
     9121 tcctcgaagt gatcttccag gagcatcttg aagcggaata gtatcctcgc ccgctcgccc
     9181 accggagtcc cggcccatcc gggaaaggct tccttcgccc ttgcgacggc ccggtccacg
     9241 tcctcgcggg tggagagcgg ggtctcggct atgacctggc cggtggcagg atcgtagacg
     9301 ggttccgtct cgcggccgtc tttctcttcc cagctaccgc ctatcaggtt tcggacctcg
     9361 aagttcctgt tctctacttg catctgcatc gcctcctcgc gcggttgttt tccctgtagc
     9421 gtctcctgta gcgagcgtcc ccgcgcaaga cgcctccatc ccccctcacc tatcgaagca
     9481 tacccgaaat agcccgggcc tggaacgttc tatccgccgc gtttcccgaa cgcctccagg
     9541 ttcttctggt agagggcgaa cggagcgttc atcaggttgg tgtaggagtc catcgcctcg
     9601 ctcatcatct tctggaacgc ctccatctgc ttctgggagc cgtccatgat gttgttggcg
     9661 acggtctggc tctcctccac gccgcgctgc agttccgagg gcgcgccgcc gaagaagttc
     9721 cgcaccaggt tctcgttacg ctcctgcatg ctcacggcct tctccaggac atcccggtag
     9781 gcgtcgaggc tctcggcgag agcgcggctg gtgcgttcct gactcttcac cgtctcgtcg
     9841 gccagcttca cgaaagactc catagcgcgg gtcagggcgc ggttggtctc ggtctggctc
     9901 tctactacgc cggtgaccgc ctcggcccag ccctgggcga gcttcacgtt gcgctgctgg
     9961 gcgtccacgg cgctcttcat gaccgtctgg taggactcgc cgaacgcctg ggccagcgtg
    10021 ttcgccgcct ccggctgttg tctctcggac atcgcactcc tcctcttccc gtaagttcat
    10081 ggttattcta cccgtccggg tggtatggtg actccccggc aacgtctacc agaagaaagg
    10141 acatcccaca tgcgtgcgat ggttacacca gagttcggag gcccggatct cttcgaagag
    10201 caagacttgc agaggccgga ggccggtccg ggtgaggtag tcgtgagggt cgtcgtggcc
    10261 gggaccaacc cggtggacgc caagctgcgc gccggggaca taccgggggc cgtggagacg
    10321 cccgtagtgc tcggcgcgga tgtttcgggc gtagtcgagg aggtcggcgc gggggttacg
    10381 gacctgaagc cgggcgacga agtgtactac tcgccggagc tcttcggccc ggtctcaaac
    10441 ggctcttatg ccgagttcaa cctcgttccg gcggccatag tagcgcccaa gccgtcctcc
    10501 atctcgcacg aggaggcggc ggccgtcccg ctcgccgggg gtacggcgtg ggaagccatc
    10561 gtgcggcggc tcggcgtggt tccgggagag acggtcctca tccacggcgg cgcgggcggc
    10621 gtgggctcct tcgcggtcca gatcgccagg gccgccggag cgcgggttat cgcgactgcc
    10681 ggctcctcta accaggagac gctgcgggaa ctcggcgccg acgtgtgcct cgactactcc
    10741 tcggatgacg tggccggaac cgcgagcgcg gagaacggcg gcgagggcgt ggacgccgtc
    10801 ttcgacacgg tcggcgggca ggtggaggaa gcgaccggct acacgcgccc cttcggtcgg
    10861 atcgccacga tcctgccgcc gcagggagac ctcacgcagc tctacctcaa gaaccagacc
    10921 ctctacggcg tcttcctaac ccgcgagcgg cgtcggctcg tcgagctggc ccggctcatg
    10981 gaccgcggcc aggtaaagcc gctggttgac gaggtgctgg acctctcgga ggtcggcaag
    11041 gcccacgagc gcctggagtc cgggcacggg cgcggcaagg tcgtgctgcg ggtgagccgg
    11101 ggttagagcg gccggcttgt cggctatcgg taatcagcga gcctgcgtaa gccggaggtt
    11161 aaataatcat taacagtgct ataggcgggg ttgacatagg ggcaccgcct cttaacattc
    11221 gtccatggac accaagcgcg gggaatcaaa ggaagctctt gccggtaagt tagaggaagc
    11281 gagggagcgg acgcggaagc tgctcgctcc cgtctcggac caggatctgg cgaggcagca
    11341 tgaccggatc atgagccccc tcatctggga ctacggtcac atcggcaact atgaggagtt
    11401 gtggctgatg gagcgtgcct tcgggcacgg cctctccgac cgcgagctct acgacatgta
    11461 cgacgcctcg ctccatcctc gcgaggagcg gccgtccctg aacctcctcg accgctccga
    11521 cgcggacgtg tacctggacg ccgtcaggaa ggcggtcctg gaatcgctgg aagcggccga
    11581 cctgaaaggc gaagacccgc tgctacacga cggtttcgtc tacaacatga ttttgcagca
    11641 cgaggcccag cacaacgaga ccatgctcca gaccctccag ctcatgcggg gcgacggcta
    11701 ccatcccgaa ccggagatag accttccgga gaaccccggc gtcgaggacg agatggttcg
    11761 tatccccgcc ggtcccttcg tcatgggcac ggacgaccgc tcgctggccc tggacaacga
    11821 gcgcgacgcc cacgaggtag acgtgccgga gttctatctg gacaggatgc ccgttacgaa
    11881 ccgggagtat ctggagtttg tggaagacgg aggatacgag cgcgaagagt tgtggaacag
    11941 cgcaggttgg aagtgggtcc agtcggagac gatagccgcg ccgaagcact ggtaccagcg
    12001 cgacccgcac tcctggtgga cgaagtgttt cggcttcgac gagccgctgc gccccgacgc
    12061 cccggtcgtc cacgtctcct ggtacgaggc cgacgcatac gcccggtgga ccggcaagcg
    12121 cctcccgacg gaggccgagt gggagaaagc agcctcctgg gaccctgaaa gcgggaccgg
    12181 gaggctcttt ccgtggggcg acgagccgcc cacaccggag aaggccaacc tggaccagct
    12241 cgccttccga acctcggatg tcggcgcgta tccggcgggc gccagcgcct acggtattct
    12301 ggacatgatc ggtaacgtct gggagtggac cgcgagcgac ttcgctccct accctagctt
    12361 tcagagcttc ccgtaccgcg agtactccga ggtcttcttc ggcccggact acaaggtctt
    12421 gcgtggcggg tcctgggcca cccgccccgt agccgtccgc aacaccttcc gcaactggga
    12481 ctacccgatg cgccgccagc tcttcgtagg cttccgctgt gcgcggtaga gcttccgtgt
    12541 tgcacgccaa aggtccgcgc ccgagagccg ggcgctagag atgtgccgca tggccgcata
    12601 cctgggcggg cctgagacct cgctctcctc agtagtgctc aagccggagc actcgctact
    12661 ggtacagagc tacgcgccga gggagatgat gagcggggtc gtgaacgccg acgggttcgg
    12721 ggtcgggtgg tacgtgccgg gcgacgatga gcccgccgtc taccgttcga actcctccat
    12781 atggtcggac cggagcttcg cggggatagc gccgaagata cggtctcaga gaatcttcgc
    12841 cgccgtccgc agcgccacgc cgggcctgcc ggtcgaggag agcggcgtcc cgcccttcga
    12901 ctccggcagg ttcatgttca tgcacaacgg cgccattcag gacttccgcc gcagggccat
    12961 gcggcccctg cgcgattccc tgagcgacga gtcccacgcc gggctgctcg gcgtctcgga
    13021 ctcggagacg atcttcgccg gtctgctcga ccggctctgg gatggcggcg acatcgctgg
    13081 cgcgctcgcc gccacgatcc ggcacgtctc cggcgtgtgc gccgtggccg gagtaaaggc
    13141 gtcgctcaac cttgcgctca cggacggaga ggcgatggcg ttcacccgcc actccaccga
    13201 gggaccgggc aactcgctgt acttcgccga gagaatggag gcgctgccgg gagcggtagt
    13261 cgtggcctcc gagcggctcg acggcgacgc gggctggcgc gaggtcccgg acaggcacct
    13321 gctacacgta accgagggaa gcgtgacgct gcgcgcgctc tgaggaggat ctcgatatga
    13381 accgggggac cagccggggg accggcaaca taaacatcac gctgctcgac gaggttgggt
    13441 cgggtgagga ccagatatcg cgggacgtcc gcgccggtct gctctccagc cccaaggacc
    13501 tctctccttg gcccaagtac ttctacgacg ccgaggggtc gcgtctgttc gaggagataa
    13561 cggagctgcc ggagtactac cagacccgcg cggaactttc gatcttgcag gatagagcga
    13621 aggagataat cgagatcact ggcagccggg aactggttga gcttggctcc ggctctgcca
    13681 gcaagacccg cgctctgctc gacgccctcc tgggctccag ggacgaaact gtctccgaaa
    13741 cgccccgcta catgccgctg gacgtgagcg agagcgccct gcgcgagagc ggcgaaaggc
    13801 tcaccgtcga gtacccgcag ctagagatac agggctacgt cggagacttc gacacctccc
    13861 tcggcaggct gctcggcccg gagagcgcca acgggtctgg ggggcggctc gtgatcttcc
    13921 tcggcggcac tatcggtaac ttcacccccg agaagcgccg ctccttcctg cggaagatac
    13981 gcgccggtct cacgcccggt gagcacgccc tgatcggggt ggacctcgtc aaggaccgcg
    14041 agactctcga agccgcgtat gacgactccg ccagagtaac agcccggttc aacaagaaca
    14101 tgctgagcgt cctgaacaac cgtctcgggg cgggtttcga gccggacctg ttcgagcacc
    14161 gcgccatcta caacgaacaa gagcagcgta tcgagatgtg gctcgactca tgcgtggatc
    14221 agaacgtacc aatccccgcc cttgggctcg acgtccgctt cgcagctggg gaagggatga
    14281 ggaccgagat cagcaccaag ttcacccctg actccgcatc agagatgctc gaagaagcgg
    14341 gcctggagct actggacctc tacaccgacg agaggaacct gttcggcctc gctctcgggc
    14401 gggccggata acggtctagt atttcgcgat aacccagacg gcgtggatga taccggggat
    14461 gtagcccagg atcgtgagaa ttacgttgat ccagaagtgc ttgccgatac cgacctggag
    14521 gaacacaccg agcgggggga gcagtatggc gactatgatg cggagaatat ccataaggga
    14581 actcctaact ccgggaactc taaaacgact atacccgccg tcatggacta ctacgtgcca
    14641 gtcctgagca agtttccgcg atctcgagta tccggttcaa acgcgcctcc gcctcttcgg
    14701 tggtggtctg tgtatgccgc cggagcgggc cggacggttt atagtgtgtg gcgaaagcga
    14761 gaaagggaag gtgagtatgg atctccgctc cgttcagaag ccgctcaagg acaggtaccg
    14821 caacgagccg ggctcgtcga agatcactct aaaggccacc ggcagcgaga cggacgctcc
    14881 gatctcctgc tccgtggaca tcgggcgcgc cgtctatgag gccgaggctc acacgggggt
    14941 cggcggcatg ggcaccgccg cctgctccgg ggacctgctc ctcggcgcgc ttgcggcgtg
    15001 cgcccagatc acctgccaga tggtcgccag cgccatgaac gtcccgacga acagcatcga
    15061 agccaccgcc gagggcgacc tggacctcgc cggcactctc ggcatctcca aagacgctca
    15121 ggtcggcttc gaggagatac gcctcaccct gaaggtggac gcccccgaag ccacatccga
    15181 acagctcgac gccctgcacg agaagaccga gcagtactgc gtcgtaatgc agaccctcgc
    15241 ccatcctccc aggctccgca ctgaatgggt ccgggagaac gaaagcgaag ccggctagtc
    15301 tctatccctc gtcccgtccg tggacctcag cgggcgggct cgtcgcctcg acagcggtga
    15361 acgtttccag gatattgtag gtgtgggaag cgcccttcgg gacgagccac gagtcaccgg
    15421 gctccagcag gaccttctgg ccctccaaat gtagctcggc gcggccgtct ataacataac
    15481 ccaccgtctc gtagtcgcgc cgggcctcgg gcttggagtc tcccggctcc tcgccgtccc
    15541 acagacgcat cgagaccgac ttgccgtccg cgagatactt ctgccccatc ccgcccttcg
    15601 gcgaagtctt cgagctaacc ttcttgatgg tcttatcttc ggtcatgaag tcctcctgat
    15661 gttgattcta tgtccgacgg cgttacccga gaagagataa aagtacactc gcaggagatc
    15721 aaacaaccag atcagagcgt gatccccaac tgctcgaagg ctctataaac tgtggcggca
    15781 ttctctaata cccttgccgt gagaccagcg aatattaaag aaagtaccaa catgtagagc
    15841 acgtatagcg ttacatatgt ccactccgac ctcatcatgc gtcgaagcct gccagggggc
    15901 agcggtggtg atccgggagc tgaggccggc atcactccaa gat
//
LOCUS       DASSMB010000091        14978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1412977, whole genome shotgun sequence.
ACCESSION   DASSMB010000091 DASSMB010000000
VERSION     DASSMB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412977"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VFJ72_05810"
     CDS             <1..706
                     /locus_tag="VFJ72_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET7479014.1"
                     /translation="STLPGKLADCSSKDPARSELYIVEGDSAGGSAKQGRDRNFQAIL
                     PLRGKILNVEKANMNKVLSNVEIQAMISAIGAGVADSFNLEGVRYNKIVIMTDADVDG
                     SHIRTLVLTFLFRNMPELIEAGYVYIAQPPLYKITHHRKDYYVYNDRELRETLTRLNA
                     NGNANIGRFKGLGEMNPNQLWETTMDPQNRTLLQVTVESAAVADELFTALMGDKVEPR
                     KAFIEANAREVKNLDA"
     gene            699..3278
                     /gene="gyrA"
                     /locus_tag="VFJ72_05815"
     CDS             699..3278
                     /gene="gyrA"
                     /locus_tag="VFJ72_05815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7479015.1"
                     /translation="MLDTFSGNIQPHSIEDEIKDSFLSYAMSVIVSRALPDVRDGLKP
                     VHRRVLYAMHDLGLQPNRPYRKSATVVGEVIGKYHPHGDSAVYDTLARMAQPFALRAP
                     LVDGQGNFGSVDGDPPAAMRYTEARLTRMATEMLRDISSDTVDFAPNFDESQREPVVL
                     PARFPNLLVNGSDGIAVGMATKIPPHNLGEVVDATIAMIDDPEMGDEELAKHITGPDF
                     PTGGVIVGLRGIKDAITTGRGSVRVQAKAHTEQIKGNRTQIVVTEIPYQVNKSYLLQK
                     IAELVKERKLDGISDLRDESDRNGMRIVIELKREAVPKVVLNNLYKHTQMQQSFGVNL
                     VALVDGVPKTLSYKKVLTHYVAHQFEVVTRRTRYELERAQARAHILEGLLIALSNLDE
                     VVAIIRKSRSVETARNNLMKTFKLSERQAQAILDLRLQRLTALERQKVEQEHRDLREK
                     IEYLESVLADDGKVYEIIKEELLEVKAAYADERRTAITAEEGVDFEIEDLIAEEEMVI
                     SISNGGYLKSVPVNTFRKQGRGGVGVAGMELKEGDYIEHLFITTTHHYMLFFTNKGKV
                     YRLKVHQLPRMSRTAKGRHIANLLPLAQGERIASVLATKEFKEAEYLLFATKNGVVKK
                     TNFLDYNTPLKNDGIIAINLAEDDELISVRHASEGAEVVIVSRNGKGIRFSQSDARPM
                     GRATGGVKGITLGKDDVVLSMDVVADDAADLFMITERGFGKRTALSQFRAQGRGGQGV
                     IAMKTDGERGRLAGVRVVRPGLHELMIVSNFGTTIRIDADSVSQQGRSAQGVRVMNLR
                     SGDEVSAIAKVVSSRSTDADGATDELALDEISGEEAINESAAGLPEAGLPEANGSGEV
                     PGA"
     gene            3271..3684
                     /locus_tag="VFJ72_05820"
     CDS             3271..3684
                     /locus_tag="VFJ72_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479016.1"
                     /translation="MHSECNGVVECTALVLAESCTEYEDRLTLFGILDEIRPRRGGET
                     SFVVYTKFEGCEHASDKERRARILITDEEGEVLMESAEYSVYLDGTEEPVTIVAAFDL
                     PDDFEVGEQLLWVEAVLDDETLAQTAVRIHDRYSV"
     gene            3744..4979
                     /locus_tag="VFJ72_05825"
     CDS             3744..4979
                     /locus_tag="VFJ72_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7479017.1"
                     /translation="MPEDLAIFIDWENIYISTVTEYNSKPNVSAILEKAREYGRIVSA
                     TAYADWTDGDFRNAPPTLYSNGISPRYISARYFPGGRSQKRRTNSIDVMLAVECSDFL
                     HNHPQVDTYVLVTGDGDFIPLVSLLRSQGKVVVVIGVSEATSYHLIESADHFISYASL
                     LEEDRAARAPDKEPKKKDSDPYVELVRAVETLKKGGKTRVLGQVKQQMIVQLGSFDER
                     NAGFKKFKDFVVEAERRGLVNTVDQDLELQVYLPDEKVPDLQEADLAAEEPKGDGAAA
                     QSAGRDRGNGKPEAKSNGKPVREESEQEPEPAAPKAGFDLLEDLTPYELKTVCKTVSN
                     LRQPASFVEIFSSIRELDERGELELSREEIGEVITAMLDSEFLDVVRQRPYEGSKADM
                     TFYALNSDKQEVKNALNGG"
     gene            complement(4976..5266)
                     /locus_tag="VFJ72_05830"
     CDS             complement(4976..5266)
                     /locus_tag="VFJ72_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479018.1"
                     /translation="METALNIGLAVVGLVPLILVFYVPALLGVAVLKERGEAYRVKAA
                     ILFAVGFGAIILAHLMLRSVSALQVVETVGVSLVQISIALALAVFTVYRLAD"
     gene            5390..5791
                     /locus_tag="VFJ72_05835"
     CDS             5390..5791
                     /locus_tag="VFJ72_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479019.1"
                     /translation="MGINQRVSLRKFVEDIRRWVEEGRSDEWIASALGTSPSSVQSFR
                     SRNGIHRRGMVSTLHEPRDFTAYEGVIEPNGPGVWFDPSVGEDERWARRWSGVDRVEL
                     RVTPTKIVLLRRGAARGARSLAQPGSPGGNL"
     gene            5834..6240
                     /gene="rnpB"
                     /locus_tag="VFJ72_05840"
     ncRNA           5834..6240
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFJ72_05840"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            6378..7133
                     /locus_tag="VFJ72_05845"
     CDS             6378..7133
                     /locus_tag="VFJ72_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479020.1"
                     /translation="MSAIAAAWKRLPWWVEPMTVVTVLTAFGIYSLTIVILFPGVYHE
                     YISPFYSPPVGRPDWLPWFITAPMFILWIPLGFRATCYYYRKAYYRAFFWDPPACQAK
                     AQQRDRPWSNDPQGYRGERALFVLNNVHRYFLYASLVVVAFLWYDTILTFFPDGSFGV
                     RLGSLIWLVNVVLISAYTFSCHSMRHLVGGNKDCYTCIRGGNARRKLYNGVSRLNLRH
                     PQWAWLSLFSLLITDVYFRLIMAGVIPDVTIIG"
     gene            7152..8963
                     /locus_tag="VFJ72_05850"
     CDS             7152..8963
                     /locus_tag="VFJ72_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7479021.1"
                     /translation="MQRDEHNNGSPNGHRENYEVREHDVLIIGAGGAGLRAAVAATQH
                     GLSVGIVTKSLLGKAHTVMAEGGMAAALGNVDPDDTWKTHFMDTMRSGKFINNWRMAE
                     IHAKESPDRVYELEQWGALFNRTPEGKISQRPFGGHSYRRLAHVGDRTGLELIRTMQE
                     KVLATDAKVYMETTVTKLLKSNGRVVGALAYTRESGKFVHYKAKAVVMATGGWGRIFK
                     VTSNSWEGTGDGVILAYDAGAELVDMEMMQFHPTGMVWPPGVRGLLVTEGVRGEGGLL
                     RNSKGERYMEKYDPEKMELSTRDVVARANYTEVQEGRGSEHGGVYLDITHLGYDGIMK
                     KLPTMHEQFLKLADIDISKEPMEVFPTVHYTMGGIKIDPETCASNVPGLFAAGECGGG
                     LHGANRLGGNSLSDLLVFGRRAGEGASAYVQESVHSADIAEAEILKEIDRVILPLNNE
                     KGKESPYLMQQELQEAMMEHANLMRDEDSLKEGLGKVLALKDRLPNLSVPGSRLFNPG
                     WHTAQDIRYLIQISEIIIRTALLRTERRGAQWRLDFDGPDDELGKINFIVKKDAQGEV
                     GIERREIPPMPQHLLSLFEDKVVDMAGGEKRGAESQF"
     gene            8994..9839
                     /locus_tag="VFJ72_05855"
     CDS             8994..9839
                     /locus_tag="VFJ72_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HET7479022.1"
                     /translation="MDSNSAEQKDMASKAHADVHADEKAVENGKTAKLRIFRGDSGGG
                     ELVDYELPLVEGMVVLDAVLYVQAHQANDLAVRWNCKAAHCGSCSAEINGKPRLLCKT
                     RIEEFDGGEIQVYPLQAFPVIKDLVSDVSWNYQVSKGIKPFTSDEQAPFTMYEEDVER
                     LYEPKKCIECFMCQDVCHVLRTHHKHAEFAGPRFFVRNQWLEMHPMDNQDRLHDLKED
                     NGIGYCNITKCCTEVCPVDIHITDNSIIPLKERVASEYYDPAKWLMRKIRGNRGSRNG
                     SRNGR"
     gene            complement(9855..9927)
                     /locus_tag="VFJ72_05860"
     tRNA            complement(9855..9927)
                     /locus_tag="VFJ72_05860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9892..9894),aa:Gly,seq:ccc)
     gene            9972..10523
                     /locus_tag="VFJ72_05865"
     CDS             9972..10523
                     /locus_tag="VFJ72_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7479023.1"
                     /translation="MNPPEYRFCPQCGGRFESRVLKDGEPERLVCGECGFVFYLGPKL
                     VAGAVFEMDGNVVLIQRAIEPGYGKWTFPGGFVERGERAETAAEREVLEECGVEISVT
                     GILGLYTYEGQIPAIAVFTADATGGEPAPLDETMDVRGFPRYALPWQEMAFPSTEHAL
                     KDYLRLGGLNLLGDSEPEGFAKR"
     gene            complement(10513..11064)
                     /locus_tag="VFJ72_05870"
     CDS             complement(10513..11064)
                     /locus_tag="VFJ72_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HET7479024.1"
                     /translation="MEPRNVLIGVTGGIAAYKAPGVIRRIREAGHEVRAVATEAAFRF
                     IPEETLSIAAGSPVHTDTTWWERSGRVEHVSLARWADLVLVAPATADAMARAAIGLGD
                     DLLSATILAGAKKVLWAPAMNPEMWHAPATVRNVGILKSWGHGFVGPDEGLMASTEEQ
                     PGVGRLADEAEILAAVQEALVNA"
     gene            13023..13367
                     /locus_tag="VFJ72_05875"
     CDS             13023..13367
                     /locus_tag="VFJ72_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479025.1"
                     /translation="MRVLVANEPRSYREAFTAAFQALRPHIESIAVEPEDLEQETIRL
                     RPDLVVCEHITPALEARVRYLMEVRVEDEVLVAMSNVPALFTATNLNLADLLSFLDQC
                     EEMMEQEVSLGR"
     gene            13713..13889
                     /locus_tag="VFJ72_05880"
     CDS             13713..13889
                     /locus_tag="VFJ72_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479026.1"
                     /translation="MVSKTHNLRWLESLKESQKITAREERRKDEILAIYQRLHRISSR
                     RLDPPVFPPWRTHW"
     gene            13955..14737
                     /locus_tag="VFJ72_05885"
     CDS             13955..14737
                     /locus_tag="VFJ72_05885"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7479027.1"
                     /translation="MERTLTGKVAVVTGASSGIGEATALALAERGASVVLAARASEKL
                     EEMERGISARGGRALSIRTDVSDPSSVEAMIERTVGEFGAIDILVNNAGLGLSGRVAE
                     LRAEDLRYVFEVNVVGPLNCIQSALPHMREGGRIINVSSVVGKRSIPKVGAYSASKFA
                     LNALSDALRVEIARRGISVTSVYPGTTRTPFRDNSRRTKDEKRGWRPKGVTPDVVAGK
                     IARAAEKGPRDVYITLKDRLFVAAVTLLPGLTDRVLGFWSRD"
     gene            14739..>14978
                     /locus_tag="VFJ72_05890"
     CDS             14739..>14978
                     /locus_tag="VFJ72_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479028.1"
                     /translation="MGFEESVTARLRRYREEADRSLSLLRETEQTARKFSGRLFAGLE
                     YAAVLGRQAGFEVETAHTGDLFMLRVAAGPGSEAGA"
BASE COUNT         3100 a         4493 c         4840 g         2545 t
ORIGIN      
        1 ctcgacgctt ccaggcaagc tcgccgattg ttcgtccaag gacccggcgc gaagcgagct
       61 atacatcgtc gagggcgact cggcgggcgg ttcggccaag cagggtaggg accggaactt
      121 ccaggcgata ctgccgcttc gaggcaagat cctgaacgtc gagaaggcca acatgaacaa
      181 ggtcctctct aacgtggaga tccaggccat gatctccgca ataggcgccg gggtcgcgga
      241 cagcttcaac ctcgaaggcg tccggtacaa caagatcgtt atcatgaccg acgccgacgt
      301 ggacgggagc cacatcagga cgctcgtgct gactttcctg ttcaggaaca tgccggagct
      361 catagaggcg ggttacgtgt acatcgcgca gccgccgctc tacaagatta cgcaccatcg
      421 caaggactac tacgtctata acgaccgcga actgcgcgag acgctcacgc gcctgaacgc
      481 caacggcaac gccaacatcg gccgcttcaa gggcctcgga gagatgaacc ctaatcagct
      541 ctgggagacg acgatggacc cgcagaaccg gacgctcctt caggtaacgg tcgagtcggc
      601 ggcggtcgcg gacgagttgt tcacggcgct catgggcgac aaggtcgagc cgcgcaaggc
      661 ttttatcgag gcgaacgcca gagaggtgaa aaacctggat gcttgatact ttctcgggga
      721 acatacagcc ccactccata gaggacgaga taaaggactc gttcctctcc tacgcgatga
      781 gcgtgatcgt ctctcgcgcc ctgccggacg tgcgagacgg cctcaagccg gtccaccggc
      841 gcgtactcta cgcgatgcac gacctcgggt tgcagccgaa tcgaccgtac cgcaagagcg
      901 cgacggtcgt cggcgaggtc atcggtaagt accatccgca tggcgactcg gcggtctacg
      961 atactctggc ccggatggcc cagcccttcg cgctgcgcgc cccgctcgtg gatggtcagg
     1021 ggaacttcgg atcggtagac ggagacccgc cggcggcgat gcgctacaca gaggcgcggc
     1081 tgacgcgcat ggcgacggag atgctgcggg acatcagctc ggacacggtc gacttcgcgc
     1141 cgaacttcga cgagagccag cgcgagccgg tcgtcttgcc agcgcggttc ccaaacctgc
     1201 tcgtcaatgg atcggacgga atcgcggttg ggatggcgac caagataccg ccgcacaacc
     1261 tgggcgaagt ggtggatgcc acgatagcca tgatcgacga cccggagatg ggcgacgagg
     1321 aactggcgaa gcacatcaca gggccggact ttcctacggg cggtgtgatc gtcggcctgc
     1381 gcggcatcaa ggacgccatc acgaccgggc gtggctcggt acgggtgcag gccaaggcac
     1441 acacggagca gatcaagggc aaccggaccc agatcgtggt cacggagatc ccgtaccagg
     1501 tcaacaagag ctacctcctg cagaagatcg ccgaactcgt caaggagcgg aagctcgacg
     1561 gcatctccga tcttcgcgac gagtcggacc gaaatgggat gcgcatagtc atcgagttga
     1621 agcgtgaagc agtcccgaag gtcgtcctga acaacctcta caagcacacc cagatgcagc
     1681 agagcttcgg cgtgaacctg gtcgccctcg tagacggcgt tccgaagacg ctctcgtaca
     1741 agaaggtgct gacgcactac gtggcgcacc agttcgaggt ggttactcgc cggacgcgct
     1801 acgagttgga gcgggcgcag gcgcgggctc acatcctgga agggctcctg atagcgctct
     1861 ccaacctgga cgaggtcgtg gcgatcatcc gcaagagccg gagcgtcgag acggcgcgca
     1921 acaacctgat gaagaccttc aagctctccg agcggcaggc gcaggcgatc ctggacctgc
     1981 ggttgcagcg tttgacggcg ctggagcggc agaaggtcga gcaagagcat cgggacctgc
     2041 gggagaagat cgagtacctg gagagcgttc tggcggacga cggtaaggtt tacgagatca
     2101 tcaaggagga gcttctggag gtgaaggccg cctacgcgga cgagcgccgg acggcgatca
     2161 cagccgagga gggcgtggac ttcgagatcg aggacctcat cgccgaggag gagatggtca
     2221 tctccatttc caacggtggc tacctgaaga gcgtgcccgt caacacgttc cgcaagcagg
     2281 gccggggcgg ggtcggcgtc gccgggatgg agctgaaaga gggcgactac atcgagcacc
     2341 tcttcatcac cacgacgcac cactacatgc tcttcttcac caacaagggg aaggtgtacc
     2401 gcctgaaggt ccaccagctc ccccggatga gccggacggc gaagggccgg cacatcgcga
     2461 acctgctgcc gctggcgcag ggggagagga tcgcctcggt gctggcgacc aaggagttca
     2521 aggaggcgga gtacctgctc ttcgcgacga agaacggcgt tgtcaagaag accaacttcc
     2581 tggactacaa cactcccttg aagaacgacg ggatcatagc gatcaacctc gccgaggacg
     2641 acgagttgat ctcggtccgc cacgcttccg agggcgcgga ggtagtaatc gtctcccgca
     2701 acggaaaggg catccgcttc tcgcagagcg acgctcgtcc gatgggccgg gcgaccggcg
     2761 gcgtcaaggg gatcacgctc ggcaaggacg acgttgttct gagcatggac gtggtcgcgg
     2821 acgacgcggc ggacctgttc atgatcacgg agaggggctt cggcaaacgc accgccctct
     2881 ctcagttccg ggctcaggga cgcggcggtc agggcgtcat cgccatgaag accgacggtg
     2941 aacgcggcag gctggcgggc gtgagggtgg tccgtccggg actgcacgag cttatgatcg
     3001 tctcgaactt tgggacgacg atccgcatag atgctgactc cgtctcgcag cagggccggt
     3061 cggcgcaggg tgtgcgcgtg atgaacctgc gctccggcga cgaggtgagc gctatagcca
     3121 aggtcgtctc ctcgcgctcc accgatgcag acggcgccac ggacgagctg gcgctggatg
     3181 agatctcggg cgaggaagcg atcaacgagt ccgcggcggg attaccggag gcgggcctgc
     3241 cggaggccaa cggcagcggc gaggtgccgg gtgcatagcg agtgtaacgg cgtcgtcgag
     3301 tgcacggctc tcgtcctggc cgagagctgc acggagtatg aggaccggct gacgctcttc
     3361 gggatactcg atgagatccg ccccagacgc ggcggcgaga cctccttcgt cgtctatacg
     3421 aagttcgagg gctgcgagca cgcctcggac aaggagcgcc gcgcccgcat actcatcacc
     3481 gacgaggagg gcgaggtcct gatggagtcg gccgagtact ccgtctatct ggatgggacc
     3541 gaggagccgg tcacgatagt cgccgccttc gacctccccg atgacttcga agtcggagag
     3601 caactgctgt gggtcgaagc cgtcctcgat gacgagaccc tcgcccagac agcagtcaga
     3661 atccacgacc gctacagtgt ctagaaaatc gacagagagg cccggatctc cgggcctctc
     3721 tcactactca aggaaaccga ttcatgcctg aagatcttgc gatattcatt gactgggaga
     3781 acatctatat ctccacggtc acggagtaca actcgaagcc gaacgtctcg gcgatactgg
     3841 agaaagcccg cgagtacggg cgcatcgtct ccgccacggc ctacgccgac tggacggatg
     3901 gagacttccg caacgcgccg ccgacgctct actcgaacgg catctccccg cgctacatct
     3961 ccgcccgcta ctttcctggg ggacgctccc agaagcgcag gacaaactcc atagacgtga
     4021 tgctcgcggt ggagtgctcg gacttcctgc acaaccaccc gcaggtggac acatacgtgc
     4081 tggtgacggg cgacggggac tttattcccc tcgtgagcct gctgaggagc caggggaaag
     4141 tcgtcgtggt tatcggggtt tcggaggcga cttcgtacca cctgatcgaa tccgccgatc
     4201 actttatctc ctacgcctcg ctcctcgaag aggaccgcgc cgcgcgcgcc ccggacaagg
     4261 agcccaagaa gaaagattcc gacccgtacg tggagctggt acgggccgtc gagacgctga
     4321 agaagggcgg caagacccgc gtcctcggtc aggtgaagca gcagatgatc gtacagctcg
     4381 gctctttcga cgagcggaac gccggcttca agaagttcaa ggactttgtg gtggaagccg
     4441 agcgtagggg cctcgtcaac actgtggatc aggacctgga gttacaggtc tacctgccgg
     4501 acgagaaagt ccccgacttg caggaggccg acctcgccgc cgaggaaccg aagggggacg
     4561 gagccgccgc tcagtctgcg ggacgggaca gagggaacgg taagcccgag gctaagtcca
     4621 acggtaagcc ggtacgggaa gaaagtgagc aggagcccga gcctgccgcg ccgaaggctg
     4681 gcttcgacct cctggaggac ctgacgccct acgaactgaa gacagtctgc aagactgtct
     4741 cgaacctccg gcagccagca tcgttcgtgg agatattcag cagcatccgc gagctggacg
     4801 agcggggcga gctggagcta tcccgcgagg agatcgggga ggtcataacc gccatgctcg
     4861 actccgagtt cctcgacgtc gtccgccagc gtccctacga aggctccaag gccgatatga
     4921 ccttctacgc cctgaactcc gacaaacagg aagtcaagaa cgctctgaat ggtggctaat
     4981 cagcgagcct gtagacggtg aagacggcca gagccagcgc tatggagatc tggacgagcg
     5041 acacgcccac ggtctcgacg acctgaaggg cggagacgct ccgcagcatc agatgcgcca
     5101 ggattatggc tccgaacccc acggcgaaga gtatcgccgc cttcaccctg tacgcctcgc
     5161 cacgctcctt caacacggca acgcccagta acgccgggac gtagaaaacc aggatcaggg
     5221 gcaccaaccc caccaccgcg agaccgatgt ttagtgctgt ctccatactc atgactccca
     5281 tacatataga gttgtagact acatcatagc tttaggtctg atggaaaatt gaacagacgt
     5341 tatgtaccct agtaaactta caccattcta cagaggcggt gggcgtaaaa tgggtatcaa
     5401 tcagagggtt tcgcttcgta agttcgtgga ggacatccgg cgttgggtgg aggagggcag
     5461 gagtgacgag tggatcgcga gcgccctcgg gacgagcccg tcgtcggtac agtcattccg
     5521 gtcgcggaac gggatccacc gccgcggaat ggtgtcgacg ctgcacgagc cgcgcgattt
     5581 cacggcttac gagggcgtga tcgaaccaaa cggtcccgga gtatggttcg atccttcggt
     5641 tggtgaagac gagcgttggg ccagacgttg gagcggagtg gaccgcgtgg aactgcgcgt
     5701 cacaccaacc aagatcgtcc ttctccgccg cggcgcggcg cgcggagccc gctcactcgc
     5761 gcagcccggc tctcccggcg ggaacttgta ggatagtctg taggtaccct gtaagctata
     5821 attcaggagg gttcgggccg tccgacccgt cgctgcggta tcggggagac ccgccgccgt
     5881 agagcaaagt ccggactcgc aaaaggccgc tcgggccaat aacccgaggg acgatgccgg
     5941 cgcaagccca tcgttacgtc aagtgcaaca gagagcagac cagccgatgg atcgcagctt
     6001 cgctgccgat cacaggtgat gggtgaaagg gtggggtaag agcccaccgg cggtatccca
     6061 aggcgaaagc cggagggacc ggccaggcaa accaccggtg cgagcaagcc aaattgcccg
     6121 gacgccgcaa ggcgccgcgg gctggtaggc gcagcctctc cagtagaggc ccggaggtat
     6181 cgaccttcgg agatggatgg cgggatagag cagaatccgg cttacgggac ggcccagccc
     6241 tctatccaag ttcaaacacc cttatatgaa tctggaatgg attcacatac aagctttctc
     6301 ctgatcctct atactgccca aacagcaaac gacttcagtt acaagaaggc acaagcatct
     6361 agaaaaggag cacaagcttg tcagccatag cagccgcctg gaagaggcta ccctggtggg
     6421 tggagccgat gaccgtagta accgtgctta cggccttcgg tatctattct ttgactatcg
     6481 tgatcttgtt ccctggggtg taccatgagt acatctctcc gttctattcg ccgcccgtgg
     6541 gccggccgga ctggttaccg tggttcataa cggcgccgat gtttatactc tggatcccgc
     6601 tgggcttccg ggcgacgtgc tattactacc gcaaggctta ctatcgggcg ttcttctggg
     6661 acccgccggc ctgccaggcg aaggcgcagc agagagaccg tccctggtcc aacgacccgc
     6721 agggctaccg gggcgaacgg gcgctgttcg tgctaaacaa cgtccacagg tacttcctct
     6781 acgcctcgct cgtggtcgtg gccttcctgt ggtacgacac gatcctcacc ttcttccccg
     6841 atgggtcgtt cggggttcgg ttggggtcgc tgatctggct ggtcaacgtg gtcttgatct
     6901 ccgcgtacac cttctcgtgc cactctatgc ggcacctcgt ggggggcaac aaggactgct
     6961 acacatgcat ccggggtggc aacgcccggc gcaagctgta caacggcgtc tccaggctca
     7021 acctgaggca tccgcagtgg gcgtggctca gcctcttctc gctgctcata accgacgtgt
     7081 acttcaggct catcatggct ggcgtaatac ccgacgtcac gatcatcggg tagcagggat
     7141 aggtggtaga gatgcagaga gacgaacata acaacggtag tccgaacgga caccgcgaga
     7201 actacgaggt ccgcgagcac gacgttctga tcatcggcgc cggcggcgcg ggactccggg
     7261 ccgcggtcgc ggcaacccag cacggcctct cggtcggtat cgtcacgaag tcgttgctcg
     7321 gcaaggctca taccgtcatg gccgagggcg gcatggcggc ggcgctcggg aacgtggacc
     7381 ccgatgatac ctggaagacg cacttcatgg acaccatgag gagcggcaag ttcatcaaca
     7441 actggcgcat ggcggagatc cacgccaagg agtcccccga cagggtctac gagctggagc
     7501 agtggggggc gctcttcaac cggacgccgg aaggcaagat ctcgcagcgt cccttcggcg
     7561 gtcactctta ccggcgcctg gcccacgtag gcgaccgcac cggcctggag ctgatccgga
     7621 ccatgcagga gaaggtactc gccaccgacg cgaaggtcta catggagacc acggtgacga
     7681 agcttctcaa gagcaacggc agggtcgtgg gcgctctggc ctacacccgc gagtcgggca
     7741 agttcgtcca ctataaggcc aaggccgtcg tcatggcgac gggcggatgg ggccgtatct
     7801 tcaaggtcac ctccaactcc tgggagggga cgggcgacgg cgtcattctg gcttacgacg
     7861 ccggagccga attggtggac atggagatga tgcagttcca cccgaccggg atggtctggc
     7921 ctccgggcgt gcgcggtctg ctcgtcaccg agggcgtgcg cggcgagggc ggtctgctcc
     7981 gcaactccaa gggcgagcgt tatatggaga agtacgatcc cgagaagatg gagctctcca
     8041 cccgcgacgt ggtcgcgcgg gctaactaca ccgaggtcca ggagggccgg gggtccgagc
     8101 acggcggcgt atacctggat atcacgcacc tgggctacga cggcatcatg aagaagctgc
     8161 cgacgatgca cgagcagttc ctcaagctcg ccgacataga catctccaag gagccgatgg
     8221 aagtcttccc gaccgttcac tacacgatgg gcggtatcaa gatagacccg gaaacctgcg
     8281 cgagcaatgt gccggggctc ttcgccgcgg gcgagtgtgg cggcgggctg cacggcgcaa
     8341 acaggctcgg cggtaactcg ctctccgacc tactcgtctt cggacgcagg gctggtgagg
     8401 gcgcgtcggc ctatgtccag gagagcgtgc attcggcgga catcgcggag gctgagatcc
     8461 tgaaggagat agaccgagta atccttccgc tgaacaacga gaagggcaag gagagcccgt
     8521 acctcatgca gcaggagctt caggaagcga tgatggagca cgccaatctc atgcgcgacg
     8581 aggactccct aaaggaaggg ctcggcaagg tgctggcgct caaggaccgg ctgccaaact
     8641 tgagtgttcc ggggtcgagg ctcttcaatc caggatggca cacggcgcag gacatacggt
     8701 acctgatcca gatctcggag atcatcatca ggacggccct gctgcgcacg gagcggcgcg
     8761 gggcgcagtg gcgcctcgac ttcgacggcc cggacgacga gctcggcaag attaacttca
     8821 tcgtcaagaa ggacgctcag ggcgaggtcg gcatagagcg gcgcgagatt ccgccaatgc
     8881 cacagcacct gttgagcctg ttcgaggaca aggtcgttga catggcgggc ggcgagaagc
     8941 gtggcgcgga gagccagttc tagccgtcag gaccagtagc taaggagcat atattggata
     9001 gcaattccgc ggagcagaag gacatggcct ccaaggcgca cgccgacgtg cacgctgatg
     9061 agaaggctgt cgagaacggg aagacggcga agctcaggat cttccgcggc gactccggcg
     9121 gcggagagct tgtcgattac gagttgccgc tggtcgaggg gatggtcgtg ctggacgcgg
     9181 tcctctacgt gcaagcccac caggccaacg acctggcggt gcgctggaac tgcaaggccg
     9241 cccactgcgg atcgtgcagc gcggagatca acggcaagcc gcgcctgctg tgcaagactc
     9301 gcatcgagga gttcgacggc ggcgagatac aggtctatcc actccaggcg ttcccggtca
     9361 tcaaggacct cgtctcggat gtctcgtgga actaccaggt gtcgaagggc atcaagccgt
     9421 tcacctcgga cgagcaggcg ccgttcacga tgtacgagga ggacgtggag cggctctacg
     9481 agccgaagaa gtgcatcgag tgctttatgt gccaggacgt ctgccacgtc ctcaggaccc
     9541 accacaagca tgcggagttc gccggtccca ggttctttgt caggaaccag tggctggaga
     9601 tgcatccgat ggacaaccag gatcggctgc acgacctgaa ggaagacaac ggcatcggct
     9661 actgcaacat caccaaatgc tgcacagagg tctgtccggt ggacatccac atcaccgaca
     9721 actccatcat cccgctcaag gagcgcgtag ccagcgagta ctacgacccg gccaagtggc
     9781 tgatgcggaa gatccggggc aacagaggtt caaggaacgg aagccggaac ggccgataag
     9841 agggcaaaag agagagcgga cgacgggctt cgaacccgcg acctccagct tgggaagctg
     9901 gcgctctacc aactgagcta cgtccgcgta aggcaatatt ctagcacgcc ggatgtagct
     9961 ctttctttcg cgtgaacccg ccagagtaca ggttttgccc acagtgcggt gggcgattcg
    10021 agagccgggt cctcaaagac ggcgagccgg agcggctggt ctgcggtgag tgcggcttcg
    10081 tgttctacct tggtccgaag ctggtcgccg gggcggtctt cgagatggat gggaacgtgg
    10141 tcctgatcca acgcgccatc gagcccggct acgggaaatg gacgttcccc ggcggcttcg
    10201 tggagcgcgg cgagcgggcc gagaccgccg ccgagcgcga ggttcttgag gagtgcgggg
    10261 tggagatctc tgtgaccggc atcctcggcc tctacactta cgagggccag atcccggcga
    10321 tagccgtctt tacggcggac gcgaccggcg gcgaaccagc gcccctcgac gagacgatgg
    10381 acgtgcgagg ctttccccga tacgccttgc cgtggcagga gatggccttt cccagcaccg
    10441 agcacgccct caaggactat ctgcgcctcg gcggactgaa cctgctggga gattcggagc
    10501 cggaagggtt cgctaagcgt tgacgagcgc ctcttgcaca gccgccagga tctcagcttc
    10561 atctgcgagg cgcccgactc ccggctgctc ctcggtagag gccatgaggc cttcatcggg
    10621 tcccacgaag ccatgtcccc aactcttcag gatcccgacg ttgcgcaccg ttgccggagc
    10681 gtgccacatc tctgggttca tggctggcgc ccataggacc ttcttcgcgc cggcaaggat
    10741 ggtcgcggag agcaggtcat cgccgagacc gattgccgct cgcgccatag cgtcagcggt
    10801 cgccggggca acgaggacga ggtcggccca gcgggccagt gatacgtgtt cgactctgcc
    10861 ggagcgctcc caccaggtcg tgtcggtgtg gaccggactt ccggcggcga tggaaagcgt
    10921 ctcctccggg atgaagcgga atgccgcctc ggtggcgacg gcccggacct cgtgaccggc
    10981 ttctcttatg cgccggatca cgccgggcgc cttgtatgcc gctataccac ccgttacacc
    11041 aatgagaacg ttccgcggtt ccatgcgagt agtatacaaa gcgcggagaa agccgcggct
    11101 cggacatggt aaggatgaac caggctcaag acgacagact actataagtt ctcattgaca
    11161 acgagctggc gggttgttac cgtccctgtt atagagtcaa cgtatcgcta gggtatcgcg
    11221 ccgactatct tgcggcgcgg ggagagtatg cccaactcaa gcgcatcgaa aagatcgggt
    11281 cctgttggct ggcactttct acgaaactct acgagggcgc tctctgaggc tttcatcgtc
    11341 tcgcgtcttc atgtgtttac tcaccctgac catcgggatg ctgatcttct ctagcgagca
    11401 ggcccgggcg cagggattgg atggaggcca gggactgatc ggtggccgcg ccgcttccca
    11461 gggcccggtc gcgcccatag cgagtccccc ggcggctagc actgcctctc agcccccggc
    11521 acagccgtcg gcgcagcagg cggaagagcc tgtcgccggc caacagcccg atcagcaaac
    11581 cggcgtcaca acgcctgcaa cacagacaaa cacaattcca cccgccagcc cgaccgcccc
    11641 agcaccggct actcaagcac cggcggatca agagatcgtt caacagcctc ccgaccgccg
    11701 ggtagcggcg caaccgcggc aggcggtcca gccgcagccc gcttctcaga acgccgtctc
    11761 gcaaccgcag ccgcagtatg ccgtaccgta caattcgaca ccaagcactc cgccggagcc
    11821 cgtcgtacgg ggagacgccg gtcccgtccc gaaagtcgaa cagcatcgga atgtacctgc
    11881 gaatcgtacg gagcaaaagc cacacgaaac agcccaaacc cagcccacag tcagaactgc
    11941 tatacctgga gatgagagaa ggcaccacga accggaccag tcggctcaac ccgaacatga
    12001 agccgccgga ccggccgcga aacacgaaaa cggcacgtcg aagtcggcgc cggaatctcc
    12061 ggtgaagcac gcacccggac atgagacgcg gccgaatgaa tctggttccc ggcccggaac
    12121 cccgaccagg acagagcctc gatacgcacc cgacgatcac cagagcggtc cgtccggcgt
    12181 caggtccaaa tcgcgcggga gcgggctccc cgagcggccg aaacacgtcg gggtggcggg
    12241 ggctcccgcg gctagatcca tcggcgattc caggaaagcg ccctctttgg cgcttctcac
    12301 aggggtaatt tccctgctgg ggagcgaggc tggcagccgg gagattggga cggtcccaac
    12361 cttcaggtcg ttggctgaac ttgccagcac tatcgttgaa caatcgcgag taactataag
    12421 cctcgtggtg gagaggatct ccggcttcgt gtccggtctc cgggcggcga gccgggtcgc
    12481 cgatctcgga cctcccggag gggtgattcc gcctctgccg ccccaacaac caatgggagg
    12541 caatgttctc tctggaggac atttctctga ttcgggttct ggatggctgc tcgccatgct
    12601 agcgctcgcg ttgctcacag ccacttgtcg aagagggata tggagcctct tcgagtccat
    12661 caagccggaa ttggtccctc aactggtatc cgagcggcct ggctgatcct tcttgctcag
    12721 gggacgtcga atgtcccctg gcccttgagt aatcgcgctt gattcgtagc gggttcggtg
    12781 cgcgtcgaac aagagcttta tgtaaggagg agagttgcac ttatggtcga agcaacatac
    12841 gcggtcgcta gttgacccgg tttcacgctg cacggagggg ctcgaacagg ggctgaatct
    12901 cggcggttgc aacggcgacc ggtacattcg cgtgccggag cgtcagggta aggagtggct
    12961 attcactacc gtcgctgtcg ggttctccgg cgacgggcgg aaattagaaa tgaggatatg
    13021 agatgcgcgt cctggttgcg aacgagccgc gttcataccg tgaggcattc acggctgctt
    13081 ttcaggcctt gcgaccgcac atcgagtcca tagccgtaga gcccgaagac ttggagcagg
    13141 agacgatccg gctccgcccg gatctggtcg tctgcgagca catcacgcct gcgctcgaag
    13201 cgcgggtgcg ttacttgatg gaggtcaggg tggaggatga ggttctggtc gctatgagta
    13261 acgtgccggc tttgtttact gctacgaact taaacctcgc cgatctactc tcattcctgg
    13321 atcagtgcga ggagatgatg gagcaagagg tgtccttggg acgatagatg gaaatcggta
    13381 gaggaactcc ggcctctccc ggacggctac gaacggcatg gccggaagtt ccggaattcc
    13441 ttcagcatgg tacaagggaa gctcgagaag gcttgaaggc acacgccgta gtctcagaga
    13501 ctcttttcgc gccgcccatc agcgtctccc ggacattttt cgcgattcgc ggcgtatcct
    13561 ggcacgaatt gcgcggtttt gtgtgggatt cgtgtcccga gatggtaata ttacggcccc
    13621 gcggagaggc ggggaagtag agaaattctt tatcgaagag gcgcgggaag ctccttgaac
    13681 agagtccaca gcgacgaaaa ggaaaaaccg tagtggtcag caagacgcac aaccttcgct
    13741 ggctggagag cctgaaggag tcgcagaaga tcacggcccg cgaggagcgg cgcaaggacg
    13801 agatccttgc gatctaccag cggcttcacc ggatttcctc gcgcaggctg gacccgccgg
    13861 tctttccgcc ctggagaacc cactggtgag ccttctcact ttgaactgaa tcctatcgcg
    13921 tacgctgacg gcacttcccg ctaagatact tctcatggaa cggactctta caggcaaggt
    13981 agccgtggtt accggcgctt cgagcggtat cggcgaggcg acggcgctcg cgctcgcgga
    14041 acgcggagcc tcagtcgtcc tggcggcgag agcttcggag aaactggagg agatggagcg
    14101 cgggatctcg gcgcgcggcg gccgggctct atccataagg acggacgtct ccgatcccag
    14161 ctcggtcgaa gcgatgatcg aacgtaccgt tggcgagttc ggagccatag atatcctcgt
    14221 aaacaacgcg ggcctcgggc tctcggggcg tgtggcggag ttgcgcgcgg aagacctgcg
    14281 ctacgtcttc gaggtgaacg tggttgggcc gctcaactgc atccagtcgg cgctgcccca
    14341 tatgcgagag ggaggacgta tcatcaacgt atcctcggtg gtggggaaga ggtccatccc
    14401 gaaagtcggg gcatacagcg cgagcaagtt cgccctgaac gccctctccg atgctctgcg
    14461 ggtggagatt gcgcgccggg gcatctccgt aaccagcgtc tatcccggca cgacgcgaac
    14521 cccgttccgg gacaactccc gccgcactaa ggacgagaaa cgcgggtggc gtccgaaggg
    14581 cgtaacgccg gacgtggtgg cggggaagat cgcccgcgcc gccgagaagg gcccgcgcga
    14641 cgtgtatatc accctcaagg accggctgtt tgtcgccgcc gtgacacttc tccccggcct
    14701 taccgaccgg gtactcggct tctggtccag ggactagctt gggcttcgag gaaagcgtaa
    14761 cggcgcggct ccggcgctac cgcgaggagg ccgaccgttc cctgtcactc ttgcgcgaga
    14821 ccgagcagac cgcaaggaag ttctccgggc gcctcttcgc cgggctcgag tacgcggcgg
    14881 tgctgggacg gcaggccggg ttcgaggtag agaccgctca taccggagac ctcttcatgc
    14941 tccgggtggc cgccgggccg ggctcggagg cgggcgcc
//
LOCUS       DASSMB010000092        17142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1412998, whole genome shotgun sequence.
ACCESSION   DASSMB010000092 DASSMB010000000
VERSION     DASSMB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 17142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17142
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412998"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..968
                     /locus_tag="VFJ72_05895"
     CDS             117..968
                     /locus_tag="VFJ72_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7479029.1"
                     /translation="MIPVAFDYEVAESVDHALQLLGEHGDEAKLLAGGHSLLPIMKLR
                     FAAPALLVDLGRITDLNYVRDEGDHLAIGAMTRHTDVERNPILQEHCGLLAYTASFVG
                     DPQVRHRGTIGGSISHGDAASDLPSALLALEASFVVKGSGGERTVAAGDFFQDYLQTD
                     LAPDEILTEIRVPKLSAGTGWSYQKFTRRAMDWAVVGVAAVVEKSNGGVGTARIGLTS
                     MGSTPLRASAAERALSGADSGSIAEATSSADEGANPSSDIAASEEYRRHLARVLTRRA
                     VEEALSR"
     gene            965..1918
                     /locus_tag="VFJ72_05900"
     CDS             965..1918
                     /locus_tag="VFJ72_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7479030.1"
                     /translation="MSEAVNGRRGALPPGVSHSEDLRGRLKEFDYLADEGLSTAIFLA
                     LKLGRPLFLEGEAGVGKTEVAKVLSRILETPLIRLQCYEGIDVYQAVYEWDYARQLLH
                     IRATEALGADEDREHLERDLYNREFLVSRPLLQALEHRGPTPPVLLIDEVDRSDDEFE
                     AFLLEILSDFAVTIPELGTVTAERPPVAILTSNRTREVHDALKRRCLYHYIEHPSFDR
                     EVEILRLRVPEAGETLARQVAAATGKMREMNLYKPPGVAETLDWTEALVALGAKELDE
                     KLVAATLGSVLKYREDQQRASATGLVNLLSGFDISGGRDGG"
     gene            1899..3059
                     /locus_tag="VFJ72_05905"
     CDS             1899..3059
                     /locus_tag="VFJ72_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7479031.1"
                     /translation="MEETVAEDDVPAAAPPMLDAAVAFGRVLRGAGLRVGTDRIVEFS
                     RALEELDVGRRDDVYWSGRVTLLSRPEDIEVYDRAFEVFWDRGGNVRKLTPLKRSTSS
                     PMPEDSVAPPKKTVEQSERGEEAVTLRYSPVEVLRSKDFALYSPEEFAELQRLLADLR
                     LSGALRRSRRLEPAPRGRHDPRRTLRGAMRTGGEAVKHRFRKARVQPRRVVLLCDISG
                     SMGSYSRALLRFLHASVISGARLEAFSMGTRLTRITKELASRDPDQALRQAAGAVKDI
                     SGGTRLGDVMKEFVDRWGQRGMARGAVVVILSDGWDRGDLGVLAEQMQRIHRLAYKVI
                     WVNPLKAAPGYKPLAGGMAAALPHVDVFLSGHNFDSLEELAHAIADAAGRDR"
     gene            3088..3402
                     /locus_tag="VFJ72_05910"
     CDS             3088..3402
                     /locus_tag="VFJ72_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET7479032.1"
                     /translation="MNPVVDKVAKWSEEGKRSALATVVKVERSGPLGPGTSMAVSEDG
                     QVVGSVSGGCVEPAVYEEAMMAIESGQPKLLTYGISDDEAFGVGLTCGGIIHIFVERV
                     DY"
     gene            3402..4244
                     /locus_tag="VFJ72_05915"
     CDS             3402..4244
                     /locus_tag="VFJ72_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET7479033.1"
                     /translation="MGDLLSRWHAALAEQSPVAIATIIEGPGLGAKVLVSPEDHVGTM
                     GNADLEKAVVETARGMLEGGKTGTTHFGPRGQRRMEDVAVFIQSFAPPPKMYVFGAID
                     FASAVARVGKFMGYRVIVCDARPVFATRERFPSADEVVVAWPDEFLKTAEVDKRSVIC
                     VLTHDPKFDVPVLKAALETDAGYIGAMGSRRTHDNRTARLKEEGVTEEQLARISSPIG
                     LDIGARTPEQTAVAIAAEVIALHTGHSGGRLAERSGPIHVPDADMPASPDAKDASEPA
                     TRNL"
     gene            4374..5237
                     /locus_tag="VFJ72_05920"
     CDS             4374..5237
                     /locus_tag="VFJ72_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7479034.1"
                     /translation="MQVPARFDYEVASTVEEAIELLRRHGPEARLLAGGHSLIPMMKL
                     RLASPEVVIDIHELDDELRYIQDDGGTLRIGALARHRDILESPLIRERYTILTDAEPT
                     IADPLVRNMGTIGGALSHADPAEDLPAAFVALGCEVVVRGPDGERTIAIDDLYVGPYE
                     TTIEPGEIMTEVRVPRAPQASAYSKVERKAGDYASAALGVALHMSGGTIEDVRIGMGG
                     VGSTTLRARRAEEVLKGERPGEELYRRAGEAAAGECDPIEDARGTPPYKRDLVRVLMG
                     RAMEKAVERTA"
     gene            5250..5726
                     /locus_tag="VFJ72_05925"
     CDS             5250..5726
                     /locus_tag="VFJ72_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7479035.1"
                     /translation="MRIEISVNQQPLVAEVEPRLLLVHFIRDVLGLTGTHWGCDTSNC
                     GACTVHLDGEPVKSCTVLAVRANGHEVTTIEGISAGADLSPLQEGFRAEHGLQCGFCT
                     PGMIMTGTAFLKNTPNPSEAEIRTAISGNLCRCTGYENIVKAVRYAAEKSREEVSA"
     gene            5726..8083
                     /locus_tag="VFJ72_05930"
     CDS             5726..8083
                     /locus_tag="VFJ72_05930"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HET7479036.1"
                     /translation="MVMAPGQGHTMGDRMPRKEDGRFIRGKGNYVDDVQLKGMLHSAI
                     LRSPFAHAKINSVDVSAALALPGVHAVIIGADLAEQGLAWMPTLAGDTQAVLATDKVR
                     FQGQEVAFVVADDRYVAQDALELIDVDYEPLDAVVDSRRALDEDAPIIRDDKEGQDSN
                     FLFFWDAGDKEATEEAFREAEVTVRQEMFYPRIHPAPMETCGAVADYDVSTGEMTIYM
                     TSQAPHAHRTLFAIVSGLPEHQIRIISPDIGGGFGNKVPIYPGYVCAAVASLVLNRPV
                     KWMESRSENLMSTSFARDYHMTGEIAATKDGKMLGVRSTILADHGAFNAHAQPGEQFP
                     TGFFPIFTSCYDVPAAYAEATGVYSNKAPGGIAYRCSFRVTEAIYLMERTMDVLADEL
                     GMDPVELRRKNFVKKEQMPYVSVMGWEYDGGDYDGALDEALKIAGYEELLEEQKKARE
                     EGRLMGIGVSTFTEIVGAGPGKKCHIAGIEMFDAAELRIHPTGKAILKLGVKSQGQGH
                     ETTFAQIVAGELGIPAENIDVREGDTDNTPFGLGTYGSRSTPVCGAATAVVARKVRDK
                     AKKIAAHMLEAAVDDLEWADGRFQIKGSPDQAKTMDEIAFAAYTNVPDGEEAGLEGVT
                     YYDPPEMTYPFGAYICVVEIDKGTGQTVVRRFIAVDDCGVRINPMIVEGQMHGGLAQG
                     IGTALMEVIEFDEEGNHLNSSFMDYLMLTSMETPNFELGEFVTPSTHHPLGARGVAES
                     PTVGSPAAVVNAVVDALSPLGVRHLDMPMTPNKVWSILREHGVGA"
     gene            8080..9021
                     /locus_tag="VFJ72_05935"
     CDS             8080..9021
                     /locus_tag="VFJ72_05935"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET7479037.1"
                     /translation="MTRPGSGDALFDRTAELAREERPFAVVTVVRTEPPTSARPGNRA
                     IVMPDGSVEGWLGGGCITPTARQEALQALESGEPRFVRISLDDAPASPGIRVVRMTCT
                     SEGSADLYVEPFLPRPALLVAGDSPVAGILAGVAAPLGFRFQALGSGETPEVPNPADT
                     WMVVATFGDYDEEAAEAGLRLGLPYVGLVASGRRAGSVLSGLRARGFAEADLETVRSP
                     AGIPLGASGHAGVAISILAEVVSLRAARRPGLSRDQAGKSTPSGTAVDPICGMTVEVT
                     SARYTTDQEGQTYYFCCAGCLDEFRSAPGKYAGRASA"
     gene            9023..9628
                     /locus_tag="VFJ72_05940"
     CDS             9023..9628
                     /locus_tag="VFJ72_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7479038.1"
                     /translation="MTGEVVGIVLGAGKSSRFGSPKQLLPFEGTTLLGRCVENANASD
                     LDRVVVVLGRASEELRRSVDFGRAEVVENTAYGTGCASSLLAGLDAAGGCDAVALLLG
                     DQPGVEPEHVNAALAEWRRERPWASVTSYRGDLGHPFIFSREAFGELRGLHGDKAVWK
                     LIEIYPERVHKVDVDADLPPDVDTPEDYELALAHRPRHRGA"
     gene            9632..10255
                     /locus_tag="VFJ72_05945"
     CDS             9632..10255
                     /locus_tag="VFJ72_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET7479039.1"
                     /translation="MPGISAILLAAGAGSRFGGGKLLAELYGRPLIEHALATLRDSPV
                     DETVVVVGPDAEKLREVCEPFGARVVENLEWELGQSTSVLAGLRAMDGGARAAVVLLG
                     DQPLVGAGAVERLITAFEAGARVAVATYGGKRRNPVLFSREVWPLLLEELSGDEGARA
                     VLRNHPDWVTEVPCDGVADPADVDTEEDLGRLKKMRGRVSDHNMGQR"
     gene            10271..11140
                     /locus_tag="VFJ72_05950"
     CDS             10271..11140
                     /locus_tag="VFJ72_05950"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7479040.1"
                     /translation="MKINNEFTVSAPVETAWDTMLDLEKVAPCLPGAAIQESTGDREY
                     KGTMKVKIGPITASYKGTVKIEEADEANHRAVINATGRDARGQGTASATIVTTMQGEG
                     DQTKVQVETDMHLTGRAAQFGRGIAQDVASKMLGQFANCLEEELAGGGLAGGESSATE
                     SAGNGASAGAAAPASDGAGSQEAPPAAPAGGAGTLGSVVSAAPGTAAAGGTSAGAVVS
                     GGSAGAAASAPTAGSGPSSGAQAAPRREPRPEPEAFDLGAASQGAILKRVGPVLAGLG
                     VLVVVIWALRRRR"
     gene            complement(11145..12422)
                     /locus_tag="VFJ72_05955"
     CDS             complement(11145..12422)
                     /locus_tag="VFJ72_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HET7479041.1"
                     /translation="MRIGGRKLHTWGYNETLPGPEIRLTEGNTLRVNARNRLPADTTI
                     HWHGVPLKNSMDGVPRVTQPPVKPGEDFVYEFVVQAAGTYMYHSHVGLQIDRGLYGPL
                     IVEPKREELDYDREYTLLLDDWLDGSPGTPEDTLKALRNSGGSMGGGMMGGESGDVDY
                     PVYLINGRAPEDPETLKVRRGERIRLRLLNSSADTIFRFAVAGHQLTVTHADGQPVEP
                     VAVDTLRIGMGERYDVMLSADNVGVWQIAAAPENKPRLARALLRCEESAGGSKSPEDS
                     RPRELKGKLLTYADLRDARGVSFPSDGLLGGPDRVHELTLSGGMGEYIWAIDGQVYPD
                     ADPLEVRKGEWVRFALRNQSMMPHPMHLHGHFFQIRNGSTHGPFKDTAIVEPNMGSLV
                     FDFVADNPGDWFFHCHNAYHMEAGMARLVSYRG"
     gene            complement(12356..12610)
                     /locus_tag="VFJ72_05960"
     CDS             complement(12356..12610)
                     /locus_tag="VFJ72_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET7479042.1"
                     /translation="MRNTMRLSNMNRREFLGLAGMSAGALALGGCGLLSPGGTLPLTP
                     RAAKTSRVRESTLEASPRKCGSAGASSIPGATMRPYPDRR"
     gene            complement(12690..12983)
                     /locus_tag="VFJ72_05965"
     CDS             complement(12690..12983)
                     /locus_tag="VFJ72_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479043.1"
                     /translation="MMSGFDGGMMGGWGAFGWLWMLVPLLFWGGLLAVIVWAVVRIFP
                     NRRMGDEPRETRTDSAEDLLRSRFASGEIDAGEYERDLAVLRGDTPRKEPNFR"
     gene            complement(13182..14321)
                     /locus_tag="VFJ72_05970"
     CDS             complement(13182..14321)
                     /locus_tag="VFJ72_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7479044.1"
                     /translation="MDDALREKAFRSLEETFANRFRRYAPGESELDADVPIASVLPQS
                     AEEVELLTELAARHSLPLAARGASTALYSGAARGVVDVRFDLMRGMRLPESGEDWVEV
                     EPGITWMVLEDTLRATGKSPTVYPTSAPRATVGGWLAENGLGVGSFEYGWLLENVLSV
                     EVVLAGGARTTVRSVDLRRLVGSRGNVGFIVGARLATRAADKDVPVAAAFRTAEDLAG
                     AVLDLYWARAPLWHAALLNPRMARARNLEEDYVLFGAYPGERATRVGPDLRKALESHG
                     GRFMSQRDAGRIWEERFFPVSPLGPTPAPGRALVSAPGLAPTLRRLEKNLPGVALQGT
                     IARDGEIWLLAFETVRQGEGLLDLNAAADDELLRVASESWRYRWR"
     gene            complement(14389..14901)
                     /locus_tag="VFJ72_05975"
     CDS             complement(14389..14901)
                     /locus_tag="VFJ72_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479045.1"
                     /translation="MKLKLIAAGTLTLALLALAPGLALAHGDETGVTVQPLTSKPDGV
                     ITVKGELLGPKSSVEVMVTGNGEDVDLGEVKANAEGDFTHEFRLPADLKPGTYQLKAT
                     GKKSATTQITVSEALAGGASSEKAGSGAMEQAPVIRQRPLRQSIGLIALFGVLAAAGM
                     FFATMRRQKA"
     gene            complement(14937..16076)
                     /locus_tag="VFJ72_05980"
     CDS             complement(14937..16076)
                     /locus_tag="VFJ72_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET7479046.1"
                     /translation="MTTNNDRTANIDQADQTSGAVPGTAGRGRLRKTWDWLDDRMGLS
                     ALRYAVPEHANTFWYTLGGITFMGIIILVVTGIWLAQYYNPDPSGARESVLFIQNTAP
                     LGDVLRGIHIWTAYLVVITALLHLGRIFVTASYKAPREVNWLVGLALLAALVFGGIFT
                     GTVLRWDQEAYEALAHNMELATLLGAFGGFFSEAFTTSVAALPRLYIAHVSMVPLILV
                     FLLIAHIFLIKHHGLSPTPAQEDAGEAPGGRLPEEKETGHYPTHLRLMIGYGLVLFAL
                     AGTLGVIFPQPIGPAPNPGIEVTKPSWVFYWLYAFEDWLGLNGILYGAILLFGILALV
                     PFIDRTPLRSLRSRPVMLVIGIVLVVALVVLSLITAFSPVVSHMG"
     gene            complement(16220..16420)
                     /locus_tag="VFJ72_05985"
     CDS             complement(16220..16420)
                     /locus_tag="VFJ72_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479047.1"
                     /translation="MIALVAMLAMLLVTAAPAMASTDVVMVSAGSAMAHAGMGVAMAH
                     AGSAHAHAAPGMVMAHFGPHQH"
     gene            complement(16520..16798)
                     /locus_tag="VFJ72_05990"
     CDS             complement(16520..16798)
                     /locus_tag="VFJ72_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479048.1"
                     /translation="MRVKRLAVVLATVVLLTVGTGVAYTGGPQDNSPTWMDQGYGQMM
                     GYGNTTGYGSMMDREDRQDMLRAMQHGDQRELQRVCLKEQREFRGDSS"
     gene            complement(16801..17052)
                     /locus_tag="VFJ72_05995"
     CDS             complement(16801..17052)
                     /locus_tag="VFJ72_05995"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HET7479049.1"
                     /translation="MMGGFGGFGMGFGIILWLAIIALIVWAVVRIFPNQGQGGNRESS
                     AGETPEETLRRRFAGGEIDAEEYERSLKVLKGERTKGGV"
BASE COUNT         2932 a         5576 c         5764 g         2870 t
ORIGIN      
        1 ccgttcaatg tgtggagcac gatccaggag gcccggggtc gctccgccgg cacgcgcacg
       61 ggcgattcct ccgtcgcgca ggcaaccgac aacactactc cgggaggtga atccctgtga
      121 tccccgtagc ctttgactac gaggtcgccg aatcggtgga ccacgccctg cagcttctgg
      181 gggagcacgg cgacgaagcc aagctcctcg ccggcggcca ctccttgctg ccgatcatga
      241 agctcaggtt cgcggccccg gcgctcttgg tcgacctcgg acgcataacc gacctgaact
      301 acgtccgcga cgagggcgac catctcgcca tcggcgcgat gacccgccac acggacgtgg
      361 aacgcaaccc catcctgcag gagcactgcg gcctgctcgc ttacacggcc tcgttcgttg
      421 gggacccgca ggtgcgccac cgcggcacca tcggcggctc catctcccac ggcgacgccg
      481 catcggacct cccctcggcc ctgctcgccc tcgaagcgag cttcgtagtg aaggggtcgg
      541 gtggcgagcg gacggtggcc gccggggact tcttccagga ctatctccag acggacctcg
      601 cgcccgatga gatcctcacg gagatccggg tccccaagct ctccgcggga accgggtggt
      661 cgtaccagaa gttcacccgc cgcgctatgg actgggccgt ggtcggtgtc gccgccgtgg
      721 tcgagaagtc caacggcggc gtcggaacgg cccgcattgg gctgaccagc atgggctcta
      781 caccgttgag ggcttccgcc gccgagaggg cgctctccgg cgcggactcc ggctcgatag
      841 ccgaggcgac cagctccgcc gacgagggcg ccaacccatc ttcggacatc gccgcctccg
      901 aagagtaccg tcgtcacctg gcgcgggtgc tcaccaggcg cgctgtcgag gaggcgctct
      961 cccgttgagc gaagcggtta acggacgccg gggggccctg ccccccggcg tttctcattc
     1021 cgaagacctg cggggccgcc tcaaggagtt cgactacctg gcggacgagg gtctgtccac
     1081 cgcgatcttc ctggccctca agctcgggcg tccgctcttc ctcgaaggcg aggcgggcgt
     1141 cggcaagacg gaggtcgcga aggtcctctc ccgcatcctg gagactcctc tcatccggct
     1201 tcaatgctac gagggcatag acgtctacca ggcggtctac gaatgggact acgcccgcca
     1261 gctactccac atccgtgcca ccgaggcgct gggagccgat gaagaccggg agcacctgga
     1321 gcgcgacctg tacaaccgcg agttcctggt gagccggccg ctgcttcagg cgctggagca
     1381 ccggggaccg acgccgccgg tcctgctcat agacgaggtg gaccgctcgg atgacgagtt
     1441 cgaggctttc ctgctggaga tcctctcgga cttcgccgtg accatcccgg agctcggtac
     1501 ggtgacggcc gagcgtccgc cggtcgccat cctgacctcg aaccgcaccc gcgaggtgca
     1561 cgacgctctc aagcggcgct gtctctacca ctacatcgaa cacccgagct ttgaccggga
     1621 ggtcgagatc ctgcgcctca gagtccccga agcgggggag accctcgccc gtcaggtcgc
     1681 cgccgcgacc ggcaagatgc gcgagatgaa cctctacaag cctcccggcg tcgccgagac
     1741 cctggactgg accgaggctc tcgtcgccct cggcgcgaag gagcttgacg agaagctcgt
     1801 cgcggccacc ctgggctccg tcctcaagta ccgcgaggac cagcagcggg cgagcgcgac
     1861 tgggctggtg aacctcctga gcggcttcga tatatctggt ggaagagacg gtggctgagg
     1921 acgacgtacc cgccgctgcg ccgccgatgc tggatgcggc ggtcgccttc gggcgcgtgt
     1981 tgcggggggc gggcctgcgg gtggggaccg acaggatcgt ggagttctcc cgagccctgg
     2041 aggaactgga tgtcggccgc cgcgacgacg tgtactggtc cgggcgcgta acgcttctct
     2101 cccgccccga ggatatagaa gtctacgacc gggcgttcga ggtgttctgg gacaggggcg
     2161 gcaacgtgag gaaactcacg cccctcaagc ggagcacctc ctcgcctatg ccggaagact
     2221 ccgtggcgcc gccgaagaag actgtcgagc agagcgagag gggagaggaa gcggtgacgc
     2281 tccgctacag cccggtcgag gtcctgcgct ccaaggactt cgccctctac tcgccggagg
     2341 agttcgccga gctgcagcga ctgctcgccg acctgcggct ctccggggcg ctccggcgca
     2401 gccgccgcct ggagcccgcg cctcgcggac gtcacgaccc gcgccgcacc ttgcgaggcg
     2461 cgatgcggac cggcggcgag gccgtgaagc atcgcttccg caaggcgcgg gtgcagccga
     2521 ggcgggtggt gcttttgtgc gacatctccg gctcgatggg ctcgtatagc cgggcgctgc
     2581 tccgcttcct gcacgcgagc gtcatctccg gcgcccggct ggaggctttc tcgatgggca
     2641 cgcggctcac gcggatcact aaagagttag ctagccggga cccggaccag gcgttgcgcc
     2701 aggcggccgg ggcggtcaag gacatctccg gcggtacgcg gctcggggac gttatgaaag
     2761 agttcgtgga ccgctggggc cagcgcggca tggcgcgcgg cgcggtcgtc gtgatcctct
     2821 ccgacggctg ggaccggggc gacctggggg tcctcgccga gcagatgcag cgcatccacc
     2881 gcctcgctta caaggtcatc tgggtcaacc cgctaaaggc cgcgcccggc tacaagccgc
     2941 tcgccggtgg gatggcagcc gctctgccgc acgtggacgt cttcctctcc ggccacaact
     3001 tcgactctct cgaagagctg gcccacgcaa tagccgacgc cgccgggcga gaccgataga
     3061 atagaggaaa agaagcagga ggtacacgtg aacccggtcg tagacaaagt cgcaaaatgg
     3121 agcgaggaag gcaagcgttc ggcgctcgcc acggtcgtga aggtcgagcg ctcggggcct
     3181 ctgggacctg ggacttcgat ggccgtctcc gaggacgggc aggtcgtggg ctcggtgagc
     3241 ggcgggtgcg tggagccggc ggtctatgaa gaggcgatga tggcgataga gagcggtcag
     3301 ccgaagcttt tgacctacgg catctcggat gacgaggcgt tcggggtggg gctgacgtgc
     3361 ggcgggatca tccacatctt cgtcgagcgg gtggattact gatgggcgac ctgctctccc
     3421 gctggcacgc cgcgctcgcc gagcagtcgc ccgtggcgat agcgacgatc atcgagggcc
     3481 ccggtctggg cgccaaggtc ctggtctcac ccgaagacca cgtcgggacg atgggcaatg
     3541 cggacctgga gaaagccgta gtggagacgg cgcgcgggat gctggaaggc ggcaagaccg
     3601 gcaccacgca cttcggaccg cgcggccagc gccgcatgga ggacgtagcc gtcttcatcc
     3661 agtccttcgc cccgccgccg aagatgtacg tcttcggggc catagacttc gcctcggcgg
     3721 tcgccagggt aggcaagttc atgggctacc gcgtgatcgt ctgcgatgcg cgtcccgtct
     3781 tcgccacccg cgagcgcttc ccgagcgccg atgaagtcgt cgtcgcctgg ccggacgagt
     3841 tcctgaagac cgccgaggtg gacaagcgca gcgtcatctg cgtcctgacc cacgacccga
     3901 agttcgacgt gccggtcctc aaggcggctc tcgaaacaga tgcgggctac ataggagcga
     3961 tgggcagtag gcggacgcac gacaaccgca cggctcggct caaggaggag ggtgtaaccg
     4021 aagagcaact cgcgcgtatc tccagcccca tcggcctgga catcggggcg cgcacaccgg
     4081 agcagacggc ggtcgccata gccgcggagg tcatcgcgct gcacaccgga cattcgggag
     4141 ggcgtctcgc cgagagatcc ggcccgatcc acgtccccga cgccgacatg cccgcttcgc
     4201 cggacgcgaa ggacgcctcg gagccagcca cccgaaacct gtaaaggagc gccactactc
     4261 aactccgcgt ggcggagaac tgctcgtgct tcttttgaca agcatcgtgc ggtgagttac
     4321 atttgaggct tcacgggggt cgatgtttcg gggctaggga aaggtggggc tgtatgcagg
     4381 tgcccgcgcg gttcgactac gaggtggcga gtaccgtgga ggaggcgata gagctgctgc
     4441 ggcggcacgg gccggaggcc cgtctgctgg cgggtgggca cagcctgatc ccgatgatga
     4501 agctccggct ggcctcgccg gaggtcgtta tagacatcca cgagttggat gacgagctgc
     4561 gttacatcca ggacgacggc gggacgctca ggatcggggc gctcgcgcgg caccgggata
     4621 ttctcgaatc tccgctcatc cgggagcgtt acacgattct cacggacgcg gagcccacta
     4681 tcgccgaccc tctggtccgc aacatgggca ccatcggcgg cgccctgtcg cacgcggacc
     4741 cggcggagga tctgccggcg gcgttcgtcg ctctcggctg cgaggtcgtc gtgcgtgggc
     4801 cggacgggga gcggaccatc gcgattgatg acctgtacgt cggaccatac gagacgacca
     4861 tcgagccggg ggagatcatg acggaggtcc gcgtgccgcg ggcgccccag gcgagcgcct
     4921 actccaaggt agagcggaag gccggagact acgcctctgc cgctctgggg gtcgcgctcc
     4981 acatgagcgg cggaactata gaggatgtgc gtatcgggat gggcggcgtg ggcagcacga
     5041 cgctccgcgc ccggcgggcc gaagaagtgt tgaagggaga gcgtcccggc gaggagttgt
     5101 accggagggc tggcgaggcg gcggcaggcg agtgcgatcc catcgaggac gcccgcggta
     5161 caccgcccta caagcgggac ctggtcaggg tcctgatggg gcgcgcgatg gagaaggctg
     5221 tggagcggac ggcttaggaa gggactgcga tgcgcataga gataagcgtc aaccaacagc
     5281 cgctggtggc ggaggtggag ccgcggttgc tgctcgtcca cttcatccgg gatgtcctgg
     5341 ggctcaccgg gacccactgg ggatgcgaca cctccaactg cggggcgtgc accgtgcatc
     5401 tcgacggcga gccggtcaag tcctgcaccg tcctggccgt ccgggcgaac gggcacgagg
     5461 taacgaccat cgaggggatc tcggccggag cggacctcag cccgcttcag gagggtttcc
     5521 gcgcggagca cgggttgcag tgcggcttct gcacgcccgg tatgatcatg acgggcaccg
     5581 ctttcctcaa gaacaccccg aacccttcgg aggcggagat ccggacggcc atctcgggga
     5641 acctgtgccg gtgcaccggc tatgagaaca tcgtgaaggc cgtccggtac gcggcggaga
     5701 agagcaggga agaggtgagc gcgtaatggt catggcgccg gggcagggac acaccatggg
     5761 cgaccgtatg ccgcgcaagg aggacggccg ctttatccgg ggcaagggca actatgtgga
     5821 cgacgtgcaa ctgaagggga tgctccattc ggccatcctg cgcagcccgt tcgcccacgc
     5881 gaagataaac tccgtggacg tctccgccgc gctcgcgctg ccgggcgtac acgccgtcat
     5941 catcggggcg gacctcgccg agcagggact cgcctggatg ccgaccctcg ccggggacac
     6001 ccaggccgtg ctcgccaccg acaaggtccg ctttcagggc caggaggtcg ccttcgtcgt
     6061 cgccgacgat cgctacgtcg cccaggacgc cctggagctt atagacgtgg actacgagcc
     6121 cctggacgcc gtggtggatt cccgcagggc cctcgacgag gacgcgccca tcatccggga
     6181 cgacaaggaa ggccaggaca gcaacttcct cttcttctgg gacgccgggg acaaggaggc
     6241 gaccgaggaa gccttccggg aggccgaggt aacggtgcgg caggagatgt tctatccgcg
     6301 catccacccg gccccgatgg agacctgcgg agcggtcgcg gactacgacg tctcgaccgg
     6361 cgagatgacc atctacatga cgtcgcaggc gccgcacgcc caccggacac tgttcgccat
     6421 agtctcgggg ttgccggagc atcagatccg gataatctcc ccggacatcg gcggaggttt
     6481 cgggaacaag gtcccgatct accccggcta cgtctgcgcc gccgtggcgt ccctcgtcct
     6541 taaccggccc gtgaaatgga tggagtcccg ctcggagaac ctgatgagca cctccttcgc
     6601 ccgcgactac cacatgaccg gtgagatcgc cgccacgaaa gacggcaaga tgctcggtgt
     6661 ccgctccacc atcctggccg atcacggcgc gttcaacgct catgcccagc cgggagaaca
     6721 gttccccacc gggttcttcc caatctttac gagctgttac gacgtgcccg ccgcctacgc
     6781 cgaggcgacc ggcgtctact ccaacaaggc tcccggcggc atcgcctacc gctgctcttt
     6841 ccgagtgacg gaggcgatct acctgatgga gcgcacgatg gacgtgctag ccgacgagct
     6901 tgggatggac ccggtagagc tccggcgcaa gaacttcgtg aagaaggagc agatgcccta
     6961 cgtctcggtt atgggctggg agtacgacgg cggcgactac gacggcgcgc tggacgaggc
     7021 gttgaagatc gcgggttatg aggagcttct tgaggagcag aaaaaagcgc gcgaagaggg
     7081 ccgtctcatg ggcatcgggg tctcgacgtt cacggagatc gtgggcgcgg ggccgggcaa
     7141 gaagtgccac atcgcgggta ttgagatgtt cgacgccgcc gagcttcgca tccaccccac
     7201 gggcaaggcg atcctcaagc tcggagtgaa gtcacagggc cagggccacg agacgacctt
     7261 cgcccagatc gtggctggag agctcggcat ccccgccgag aacatcgacg tccgcgaggg
     7321 ggacaccgac aacacgccgt tcggcctggg cacttacggc agccgctcca cgccggtctg
     7381 cggtgccgcg acggcggtcg tcgcccgcaa ggtgcgcgac aaggcgaaga agatcgccgc
     7441 tcacatgctc gaagccgctg tggacgacct ggagtgggcc gatggacggt tccagatcaa
     7501 gggctctccc gatcaggcga agacgatgga tgagatcgcc ttcgccgcct acaccaacgt
     7561 cccggatggc gaggaggccg gcctcgaagg cgtgacctac tacgacccgc cggagatgac
     7621 ctacccgttc ggcgcgtaca tctgcgtcgt cgagatagat aagggtacgg ggcagacggt
     7681 ggtcaggcgc ttcatcgcgg tggacgactg cggggtgagg atcaacccga tgatcgtgga
     7741 gggccagatg cacggcggcc tcgcgcaggg catcgggacc gccctcatgg aggtcatcga
     7801 gttcgacgag gagggcaacc acctcaacag cagcttcatg gactacctga tgctcacttc
     7861 gatggagacc cctaacttcg agctgggtga gttcgtgacc ccctccacgc atcatcccct
     7921 gggcgcccgg ggcgtcgccg agtccccgac ggtcggctct cccgccgccg tcgtgaacgc
     7981 cgtggtggac gcgctatcgc ccctgggggt gcgccacctc gacatgccga tgacgccgaa
     8041 caaggtctgg agcatcctgc gggagcacgg ggtgggagcg tgacccggcc cggctccggc
     8101 gacgctctat tcgaccggac ggcggagctc gcgcgagagg agcggccgtt cgcggtcgtg
     8161 acggtggtcc gcaccgagcc gccgacctcc gcgcgccccg gcaaccgggc gatagtcatg
     8221 cccgacggga gcgtcgaggg ctggctcggc ggcggctgca taacccccac ggcccgccag
     8281 gaagccctgc aagccctgga gagcggcgag ccccggttcg tcaggatctc actggacgat
     8341 gctccggcat cgcccggcat ccgcgtcgtc cgcatgacct gtaccagcga gggatccgcc
     8401 gatctctacg tcgagccgtt cctcccgcgc ccggccctgc tcgtcgccgg ggactccccg
     8461 gtcgccggga ttctggcagg ggtcgccgcg cctctgggct tccgttttca ggccctgggt
     8521 tccggagaga cgccggaggt cccgaacccc gcggatacgt ggatggtcgt cgcgaccttc
     8581 ggagactatg acgaggaagc ggccgaggcg gggctccggc tcgggctccc ctacgtcggt
     8641 ctcgtcgcca gcgggcgccg ggcggggagc gtgctctcgg gtctgcgcgc ccgcgggttc
     8701 gcggaggcgg acctcgaaac cgtgcgctcc ccggcgggca tccctctggg agcgagcggt
     8761 cacgcggggg ttgctatctc gatcctggcc gaggtcgtat ccctgcgggc agcgcgccgt
     8821 cccggcctct ccagagacca agccggaaag tccacccctt ccgggacggc cgtggacccc
     8881 atctgcggca tgactgtaga agtgaccagt gcccggtata cgacagatca agagggacag
     8941 acctactatt tctgctgcgc gggctgtctc gatgagttcc ggtccgcgcc cggcaagtac
     9001 gcgggccgcg cctccgcctg atatgacggg agaggtagtg gggatagtcc tgggcgccgg
     9061 gaagtccagc cggttcggct ctccaaagca actcctgccc ttcgagggga cgaccctgct
     9121 cggccggtgc gtcgagaacg ccaacgcctc ggacctggac cgggtcgtgg tggtcctggg
     9181 ccgggcctcg gaagagttgc ggcggtcggt ggacttcggc cgggccgagg tggtggagaa
     9241 caccgcctac gggaccggat gcgcctcgtc gctgctcgcg ggcctggatg ccgccggagg
     9301 gtgcgacgcc gtcgcgctgc tcctcggaga ccagccgggc gtcgagccgg agcacgtgaa
     9361 cgcggccctc gcggagtggc ggagggagcg gccgtgggcg tcggtcacgt cgtaccgggg
     9421 agacctcggg caccccttca tcttctcgcg tgaggcgttc ggagagctgc ggggtctgca
     9481 cggggacaag gcggtgtgga agctcatcga gatctatccg gagcgggtgc ataaggtgga
     9541 cgtggatgcc gacctgcccc cggacgtgga cacgcccgag gactacgaac ttgccctcgc
     9601 tcaccggccg cgccaccggg gggcataggc gatgccaggc atctccgcta tcctgctggc
     9661 ggccggagcc gggagcaggt tcgggggcgg caagctcctc gcggagttgt acggaaggcc
     9721 cctgatcgag cacgccctcg ccacgttgcg ggattcaccc gtggacgaga cggtcgtcgt
     9781 ggttggcccg gacgcggaga agttgcgcga ggtctgcgag ccgttcgggg cgcgcgtggt
     9841 ggagaacctg gaatgggagc ttgggcagtc cacctcggtg ctggccggac tccgggcgat
     9901 ggatggtggc gctcgggcgg cggtggtgct tctgggggat cagcccctcg tgggcgcggg
     9961 agcggtggag cgcctgataa cggcgttcga ggccggagct cgggtagcgg tggcgaccta
    10021 tggtggcaaa cggcgcaacc cggtgctatt ctcgcgcgag gtgtggccgc tgctcctaga
    10081 ggaactttcg ggggatgagg gggcgcgggc ggtgctgagg aatcatccgg actgggtgac
    10141 cgaggtgcca tgtgacgggg tggctgaccc cgcggatgtg gatacggagg aagatctggg
    10201 gcgtttgaaa aagatgcgcg gaagggtatc cgatcacaat atgggacaac gttaaggagg
    10261 aagccgtttt atgaagataa acaacgaatt taccgtcagc gctccggtcg agacggcctg
    10321 ggacaccatg ctggacctgg agaaggtcgc gccctgcttg ccgggagcgg cgatccagga
    10381 gtcaacgggc gaccgcgagt acaagggcac gatgaaggtg aagatcggcc cgatcaccgc
    10441 cagctacaag ggcacggtaa agatcgagga ggccgacgag gccaaccacc gggcggtcat
    10501 caacgccacc ggccgcgacg ctcgcggtca gggaacggcc tcggcgacca tcgtcaccac
    10561 catgcagggc gagggcgacc agaccaaagt ccaggtggag accgacatgc acctgaccgg
    10621 gcgcgccgcc cagttcgggc gcggtatagc ccaggacgtg gcctcgaaga tgctcggcca
    10681 gttcgctaac tgcctggagg aggagctggc cggcggcggg ctggcgggtg gcgagtcttc
    10741 ggcgaccgag tctgcgggga acggcgcctc cgccggcgcg gctgcgccgg cgagcgatgg
    10801 cgcgggatct caggaagcgc ctcccgccgc tcccgcgggc ggcgccggga cgctcgggag
    10861 cgtggtcagc gcggcgcccg gtaccgcagc ggccggtggg acttccgcgg gagcggtcgt
    10921 atcaggagga tcggcgggag ctgcggcgag cgctccgacc gccgggagcg gaccgtcgtc
    10981 gggcgctcag gcggcgccgc gccgcgagcc gcggcccgag ccggaggcgt tcgacctcgg
    11041 cgccgcgagc cagggggcga tcctgaagcg ggtcgggccg gtgctcgcgg ggctcggggt
    11101 gctggtggtc gtgatctggg cgctccgccg cagacggtag acgctcaacc cctgtaggag
    11161 accaggcggg ccattccagc ctccatgtgg taggcgttgt ggcagtggaa gaaccagtct
    11221 ccggggttgt ccgcgacgaa gtcgaagacc agagagccca tgttcggctc gacgatggcc
    11281 gtgtccttga acgggccgtg ggtcgagccg ttcctgatct ggaagaagtg gccgtgcagg
    11341 tgcatggggt gaggcatcat gctctggttc cgtagcgcga accgcaccca ctcgcccttg
    11401 cgcacctcca gggggtcggc gtccgggtag acctgaccat caatggccca gatgtactct
    11461 cccatgccgc cggagagcgt tagctcgtgc acgcggtccg ggcctccgag caggccatca
    11521 gatgggaaag aaacccctcg cgcgtcgcgc aggtcggcgt aggtaagaag cttgcctttt
    11581 aactcccgcg gccgggagtc ctccggagat tttgatcccc cggcgctctc ctcgcagcgg
    11641 agcagggcgc gggcgagacg aggcttgttc tcgggcgcgg cggcgatctg ccagacgccg
    11701 acgttatcgg cggagagcat aacatcgtag cgctcgccca tcccgatcct gagagtgtct
    11761 accgcgaccg gctcgaccgg ctggccgtcg gcgtgggtga cggtcagttg atgtccggcc
    11821 acggcgaacc ggaagatcgt gtcggccgaa gagttcagaa gacgcaggcg tatacgctcc
    11881 ccgcgccgga ccttcagggt ctctgggtcc tccggcgcgc ggccgttgat caggtacacc
    11941 ggataatcca cgtctcccga ttcaccgccc atcatccctc cgcccatcga gccgccgctg
    12001 tttcggagcg ctttcaaggt gtcttccggg gtgccgggag agccgtccag ccagtcatcg
    12061 agcaggagcg tgtattcccg gtcgtagtcc aactcttcgc gctttggttc gacgatcagg
    12121 ggcccgtaga gtccccggtc gatctgaagc ccgacatggg agtggtacat gtatgtcccg
    12181 gcggcctgaa cgacgaactc gtagacgaag tcctcacccg gctttacggg aggctgggtt
    12241 acgcgcggca cgccgtccat cgagttcttc aacgggactc cgtgccagtg tatggtcgta
    12301 tccgccggga gacggttacg cgcgttcacg cggagggtgt ttccctcggt gagccttatc
    12361 tccggtccgg gtaaggtctc attgtagccc caggtatgga gcttgcgccc gccgatccgc
    12421 acttcctcgg agaggcttcg agagtggact ccctgacgcg gctcgtcttc gcggctcgcg
    12481 gcgtcagggg tagagtgcct ccgggggaca gtaggccgca gccgccgaga gcgagcgctc
    12541 ccgcgctcat cccggccagg ccgaggaact ctcggcggtt catgttcgaa agtctcatgg
    12601 tgttcctcat ctaccgtttc ccgtcacgtg aggagcgccg gcgtccgctc catggatacc
    12661 ggcgctcgac tgttggtgtc agctttcagc tagcgaaagt ttggttcttt acggggagtg
    12721 tctccgcgca ggactgccag atcgcgctcg tactccccgg cgtcgatctc gccgctggcg
    12781 aagcgggaac gcaagagatc ctccgccgag tctgtccgcg tctcccgtgg ttcgtcgccc
    12841 atgcgccggt tggggaaaat cctcacaacg gcccagacga tcaccgccag aagcccaccc
    12901 cagaacagaa gcggcaccag catccacagc cagccgaagg ctccccatcc acccatcatt
    12961 ccaccgtcga atccgctcat catgatcttt catctccttt cttatatctc tttacggccc
    13021 cagtctaagc gccctgtttc aagcccccgt tatgcctgga tcaagtttcg atcaagattc
    13081 ttcgaccaga attccgaaac ttccggcaag agagaaaccg gaagggcggc tcggtaatga
    13141 cttttggatg atgttgcggg aaggcggttc tcgaccaacc tctaccgcca cctatatctc
    13201 caagattcgc tagccacccg gagcaactca tcgtccgcag cggcgttcag gtccagcaaa
    13261 ccttctccct ggcggaccgt ctcgaaggcc aggagccaga tctcgccgtc acgggcgatt
    13321 gttccctgta gcgcaactcc cggcaggttc ttctccagcc ttcttaacgt cggggcaaga
    13381 cccggagccg agaccagcgc gcgtcccggt gccggggttg gtcccagtgg gctgaccggg
    13441 aagaacctct cttcccagat gcggccggcg tctctttggg acataaaacg tcctccatgc
    13501 gactctagtg cttttcggag atctggccct actcttgttg ccctctctcc ggggtaagct
    13561 ccgaagagga cgtaatcttc ttcgaggttc ctggcgcggg ccatccgggg gttcaggaga
    13621 gccgcatgcc agagcggcgc cctggcccag tagagatcca ggacagcgcc ggcaaggtcc
    13681 tcggcggtgc ggaaggcagc cgcaaccgga acgtctttgt ccgccgcgcg ggtggcgagc
    13741 ctcgctccta cgataaaccc gacgtttccc cgggatccca cgaggcgcct caagtcgacg
    13801 ctcctcaccg tggtccgcgc gcctccggcc agcacgacct cgaccgagag aacgttttcc
    13861 aggagccagc cgtactcgaa cgagccgact ccgagtccgt tctcggccag ccatccgccg
    13921 acggtagccc tgggcgcgct cgtcgggtag accgtgggac ttttcccggt ggcgcgaagg
    13981 gtgtcctcta ggaccatcca ggtgatcccc ggctcgacct cgacccaatc ctcccccgac
    14041 tcgggaagcc gcatcccgcg catcaaatcg aaccggacgt cgacgacccc tctcgcagcg
    14101 ccggagtaga gcgccgtgct ggcgccccgg gccgccagtg gcagggagtg gcgagcggcc
    14161 agttcggtca gcaactccac ctcctcggcg ctttgcggga gaacggaagc gatagggaca
    14221 tcggcgtcca actcgctctc accgggcgcg tacctcctga atcggttggc gaacgtttct
    14281 tccagactcc tgaaagcttt ctcgcggaga gcgtcgtcca taggtcgcct ttctagagtc
    14341 ttggggtacc ccggcctata tttatcgaac accaccgttc catctcgtct aggctttctg
    14401 gcgtctcatc gtggcgaaga acatccccgc ggcggccagc acgccgaaga gagctatcag
    14461 accgatgctc tggcgcagcg gacgctgccg gataaccggg gcctgctcca tcgcgccgga
    14521 accggccttc tcggagctcg cgccgcccgc aagggcttcc gagaccgtga tctgggtagt
    14581 cgcgctcttc ttgccggttg ccttgagctg gtaggttccg ggcttgaggt cggcgggcag
    14641 cctgaactca tgcgtgaagt ccccttccgc attggccttg acttcgccca ggtccacgtc
    14701 ttcgccgttc ccggtgacca tgacctcgac gctgcttttc ggaccgagca actcgccttt
    14761 cacggttatg actccgtcgg gtttcgacgt tagcggctgg accgttactc cggtctcgtc
    14821 tccgtgggcc agcgcgagac ccggcgcgag tgccaggagc gccagggtta gggtgcctgc
    14881 tgcgatcagt ttgagtttca tttagatctc ccttcgagct tcttttccga gcacccctat
    14941 cccatgtgcg acaccacggg gctgaaggcg gtgatgagcg acaggaccac cagcgccact
    15001 accagcacga tcccgatgac cagcatcacg ggcctgcttc tcaggctgcg cagcggcgtc
    15061 cgatctatga agggcacgag ggcgaggatt ccgaagagca ggatcgctcc gtatagaatg
    15121 ccgttcagac cgagccagtc ttcgaatgca tagagccagt agaacaccca cgaaggcttg
    15181 gtgacctcga tgcccggatt gggcgccggg ccgatgggct gcgggaagat cacgcccagc
    15241 gtaccggcca gcgcgaagag gaccagcccg tagccgatca tgagccgcag gtgggtcggg
    15301 taatggccgg tctccttctc ttcgggcagc cgtccgccgg gagcctcgcc cgcgtcctcc
    15361 tgggccggtg tgggagagag gccgtggtgc ttgatgagga agatgtgcgc gatcagcagg
    15421 aacaccagga tcaaggggac catgctcacg tgggcgatgt acaggcgcgg tagagcagct
    15481 acgctcgtcg taaaggcttc ggagaagaac cccccgaagg cgcctagcag ggtcgcaagc
    15541 tccatgttgt gggccagggc ctcataggct tcctggtccc agcgcaggac tgtgccggtg
    15601 aagatgccgc cgaacaccag agcggccagc agcgccagtc cgaccagcca gttgacctcg
    15661 cgcggagcct tgtaagaggc ggtgacgaag atcctgccca ggtgcagcag ggcagtgatc
    15721 accaccaggt aagctgtcca gatgtggatg ccccgtagaa catcgcccag aggggcggta
    15781 ttctggatga acagcacgct ctcccgagcg cccgatggat cgggattgta gtactgggcg
    15841 agccatatcc cggtgacgac caggatgatg atccccatga aggttatccc gccgagcgtg
    15901 taccagaagg tgtttgcatg ctcgggaacc gcgtacctga gcgccgagag gcccattcgg
    15961 tcatccagcc agtcccacgt tttccgaagc ctacctcgcc cggccgttcc ggggacggct
    16021 ccgctcgtct gatctgcctg atctatattt gccgtgcgat cattgttcgt cgtcacggtt
    16081 tgctcccttc gcggggcctt tcccctaccc cgccgttacc gccaacctac acttctggaa
    16141 tctctacgtt cgctacctct ctttcgcgga gcgtaccgcg gtccgtgcta cggcggagtt
    16201 gcacaagcct cttcagacgt tagtgttggt gcggaccgaa gtgcgccatg accatacccg
    16261 gagccgcgtg cgcgtgcgcc gatcccgcgt gagccatggc tacgcccata cctgcatgcg
    16321 ccatcgccga cccggcgctg accatcacca catccgtact cgccatcgcg ggagcggcgg
    16381 tcaccagcaa catcgccagc atggcgacca gtgcgatcat cttcctcatc atgtcctcgc
    16441 ttccatctcg atttcgctcc taccgaaaga ccttgctgaa aatatggttg gttctcgcct
    16501 ccttatagtt cgggtctggc tagctggagt cgccacggaa ttcccgttgc tctttcaggc
    16561 agacccgctg caactcgcgc tgatctccat gctgcatggc ccggagcatg tcctgccgat
    16621 cttcccggtc catcatggag ccatagcccg tcgtgttccc ataacccatc atctgaccgt
    16681 agccctgatc catccacgtg ggagagttgt cctgcggacc tccggtgtag gccacgcccg
    16741 tgcccaccgt gagtagcacg accgtagcca ggacgaccgc caaacgcttg acacgcatcg
    16801 ctacacacca cctttcgtcc gttcgccttt cagcactttc agcgaccgtt cgtactcctc
    16861 ggcgtcgatc tctcctccgg cgaagcgccg gcgcagagtc tcctcgggtg tctccccggc
    16921 ggagctttca cggttgccgc cctgaccctg gttcgggaag atacgcacca ccgcccacac
    16981 gattagggcg atgatagcca accagaggat gatcccgaag cccataccga aacctccaaa
    17041 tccgcccatc atctctaccg tctcctttcc actcttgcct tacaacagca gtgtagaagg
    17101 ccggtctcaa gctaccgtcg agttcggatc aagcttcgat ca
//
LOCUS       DASSMB010000093        16323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_526281, whole genome shotgun sequence.
ACCESSION   DASSMB010000093 DASSMB010000000
VERSION     DASSMB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16323
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526281"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1003
                     /locus_tag="VFJ72_06000"
     CDS             137..1003
                     /locus_tag="VFJ72_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479050.1"
                     /translation="MQSSSEAPSRKVPTAAESLPGKSRSRRKGWARLPVTVRYVLLLA
                     VFVGAWQLYVTLAHVSPLALPGPLDVARAFWDGWASGRLADATLTTLRVLELGMLVGV
                     ILASVFTVFATWTTVGADILSLLTAMFNPVPAIAMLPILILWFGINSTALAVVIVYSI
                     VWPIAINLSAGFKTVNPTIMMVGRNLGLRGWSMVRDVLLPAALPHTITGLRTGWAFGW
                     RTIVAAELVFGVAGKGGGLGFFINDARYFLRILEVFAGILTIALIGILVEAVFNRVER
                     RTVVRWGMKSAA"
     gene            1035..2060
                     /locus_tag="VFJ72_06005"
     CDS             1035..2060
                     /locus_tag="VFJ72_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7479051.1"
                     /translation="MDTRTGRIAREYSRQEFLSLGIAAAGAGLMFAGCSASDAAGGGG
                     GSEADKLRITYQPGTTTYAQLVIMEQEGWLADDLQDVDISWNQVDAGAAVRDAIASGK
                     TDIGAGGIGPFLVGYDSGIDWRILSALDNVSMWLMVKDEKFKSLKDFKEGDKIAVVAP
                     DSIQAVILRKGAQEQLGDPHALDGNMISLPSPDSVTALLNGQVAAHFTAPPFQFQEQD
                     KGARPILKSFDLFGEHNLISLWVLRDFHEANPKIMSAVYKNVQKATKLIQDDPNRAGE
                     ILAKVSKIDPAEETRYLTEQGVSFTTTPRGFISFAKFMKTADLIKKVPGSWKDLVYDN
                     IKNSDGS"
     gene            complement(2131..3015)
                     /locus_tag="VFJ72_06010"
     CDS             complement(2131..3015)
                     /locus_tag="VFJ72_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7479052.1"
                     /translation="MTRLLLVGIAGLIAQLVDGALGMSYGVTSTTLLLAAGIAPALAS
                     ASVHIAEVGTTAASGLSHWRFGNVDWSKVFWLAIPGAVGAFLGAVVLSSVISAEAAEP
                     IIAVFLFLLGIYILARFTFERAGNLINDRPIARAFLAPLGFVAGFLDAVGGGGWGPIS
                     TPTLLSSRRMEPRKVVGTVDTSEFIVALAASAGFLLTLNFNQIPFQLVGAILVGGIIA
                     APIAAWVVRHLNARVMGTAVGGFILLTNANTFLGAIGFDPSVNIPVYIAIVAVWVSAL
                     YLAISTTRRERKPVLETS"
     gene            complement(3207..4181)
                     /locus_tag="VFJ72_06015"
     CDS             complement(3207..4181)
                     /locus_tag="VFJ72_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE-like domain-containing protein"
                     /protein_id="HET7479053.1"
                     /translation="MGITARNVSKRYGDFVALDDVSVEAPTGSLTALLGPSGSGKSTL
                     LRAIAGLERPDSGQIFISDEEKTNMPPQKREVGFVFQHYAAFKHMTVFDNVAFGLKIR
                     KHPRGAIDKKVHELLELVQLDGFSHRYPTQLSGGQRQRMALARALAVEPEVLLLDEPF
                     GALDARVRQELRAWLRRLHDDIHVTTILVTHDQEEAMEVADRIVLMNHGKIEQVGGPQ
                     DLYEHPANEFVMSFIGPVNRLGNTYVRPHDIEIKLSPNGTTSEATVDHLVYLGFEVRV
                     ELTLKDGQSLWAQVTREDARHLELERGKHIHVKIDRTKVFGEIVESPV"
     gene            complement(4181..4975)
                     /locus_tag="VFJ72_06020"
     CDS             complement(4181..4975)
                     /locus_tag="VFJ72_06020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HET7479054.1"
                     /translation="MTASRITLRTLALGYLALLLVLPVSMVFVRTFSNGLGAVWEAVT
                     RPEALHALWLTLLVAVIAVTANTVFGIVCALVLVRHEFRGKALLNAVLDLPLAVSPVV
                     VGLALLIIYGRTSLVGGWLAEAGIRIVFSTPGIILATIFVSLPFVVREVVPVLREIGT
                     EQEQAASTLGASAFQTFRLVTLPAIRSGVAYGIVLTTARAIGEFGAVSLVSGKLAGRT
                     ETLTIYVEDRFQSFDLVGCYTAAVVLAVIAVATLLLTNLTSPKEEY"
     gene            complement(4965..5801)
                     /gene="cysT"
                     /locus_tag="VFJ72_06025"
     CDS             complement(4965..5801)
                     /gene="cysT"
                     /locus_tag="VFJ72_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HET7479055.1"
                     /translation="MSVTSNTQTAPAAGSRASKREGSALTRGAAVGYLSLLVLIPLAA
                     VVWRSTEGGIEGFVRAITNPQALAALRLTFFASLAVVAINVVMGTLIAWVLVRDSFRG
                     KGLVNSIIDLPFALPTIVAGLTLLALYGPRSPLGLNVAYTKVSVGMALLFVTLPFVVR
                     AVQPVLMEIDRDMEEAAASLGASGFVTFRRIILPNLMPAILSGAALGFARSVGEFGSV
                     VLITGNQPFKTQVASVYIFGQVESGNTAGAAAVSVVLLVVSLLVLFGMSLVNRRGTRH
                     DR"
     gene            complement(5798..6778)
                     /locus_tag="VFJ72_06030"
     CDS             complement(5798..6778)
                     /locus_tag="VFJ72_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7479056.1"
                     /translation="MALIALLAVAGCGGGSSSAQGGGGGGNLTIVAYSTPREAYDKLI
                     PAFQKTDAGKGVKFDTSYGSSGEQSRAVEGGLPADVVEFSLAPDMDRLVKAGLVDSNW
                     NKNEYDGMVTDSVVVIAVRKGNPKGIKDWDDLIKPGVEVITPNPFTSGGAKWNIMAGY
                     GAQIKQGKSDQEGIDYLSKLFHNVPVQDKSAREALATFTGGKGDAMLAYENEAILAQK
                     EGEDLDFVVPDQTILIENPVAVTSDSQAPDQAKAFVKFLYTEKAQRTFGNTGYRPVDK
                     KVLGEFDYPEPSGLFTIKDLGGWDKVNDKFFDPDKGVMAKINREVGAPTE"
     gene            6990..7613
                     /locus_tag="VFJ72_06035"
     CDS             6990..7613
                     /locus_tag="VFJ72_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HET7479057.1"
                     /translation="MKQVEFYYDLVSPYSYLAYGRLREICAEQGAELKLRPMLLGAVH
                     KATGNQAPIEVEAKGRYQYRDIHRWADYYGLSLRFPDPFPFRTLKTMRAAMFCEARGE
                     LEAFTREGFALYWEEGGAPKSLAEADEDGPVSEISRRIGADSEEVLEGASGQEAKSAL
                     KEATTAAVERGVFGAPAFFVGEEMFWGNDRLHFVEAALGESRETVAR"
     gene            7721..9064
                     /locus_tag="VFJ72_06040"
     CDS             7721..9064
                     /locus_tag="VFJ72_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479058.1"
                     /translation="MERLGGEQIGQTSSIRQVALASFIGTAIEWYDFFLYGTAAALIF
                     GKLFFPEFSSTAGTLAAFSTYAVGFAARPLGGIVFGHFGDRIGRKSMLILSLMIMGVA
                     TALIGLLPTFESIGVLAPILLVVLRFCQGIGVGGEWGGAVLMAVEHAPQGRRGFYGSW
                     PQMGVPAGLLTGNLVFLAATAVLPEAWGWRVPFLFSFVLVGVGLFIRLRILESPAFTR
                     VKESGTEAPMPILDVLRTYPKNVLLAMGMRFAENGTFYVLTVFVLTYVVEELKLEQTT
                     GLIGVLIAAFIGLFTVPFFGHLSDRVGRRPLYLLGSGFSLLFAFPFFWMLNTGSAPLI
                     WLAIILGVNVGHDAMYGPQASFFAELFGTRVRYSGASLGYQLASVFAGGLAPLIALYL
                     LTTFGYGAVAAYTALMALITVISVILVTETYQSDIHQTQAEERRLISDRAEPRTQ"
     gene            9202..9990
                     /locus_tag="VFJ72_06045"
     CDS             9202..9990
                     /locus_tag="VFJ72_06045"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HET7479059.1"
                     /translation="MSEAYVRPGQSAVSVDLTGKSALVTGAASGIGKACAERLANAGA
                     EVTVLDLNGDAAREVADSIGGQSVQADLADYEVVDSLDIEADIVVNNAGLQHVAAIED
                     FPPERYSMILRIMLEAPFRIVHKTLPGMYEKGWGRVVNISSVHGLRASPFKSAYISAK
                     HGLEGLSKVIALEGGPHGVTSNCICPAYVRTPLVEGQIADQARTRGISESEVIEKVML
                     TSPAIKRLVEPEEVAELVAFLCTPEASFISGASLTIDGAATAQW"
     gene            10027..12000
                     /locus_tag="VFJ72_06050"
     CDS             10027..12000
                     /locus_tag="VFJ72_06050"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HET7479060.1"
                     /translation="MTETVEGRLLWEPSEEFKQNANVTRYMEWLRDEKDHDFADYAAL
                     WEWSVTDLEGFWESVWEYCGVKAAKPYSAVLKDRKMPGAEWFAGAELNYAEHAFLKAR
                     DDEPAIISQSEIRPMSEMSWGELRAKTAALAAGLREMGVERGDRVVAYIPNVPEALVA
                     LLACASLGAVWSSCSPDFGVGSVVDRFKQIEPKVLLAVDGYRYGGKDYDRTDVVARLQ
                     REIPSLQKTIVLPYLNDDPDTSGLEDVVLWDEVLASNEGAELTFEPVPFDHPLWVLYS
                     SGTTGLPKAIVQSQGGILLEHLKALILHMDLAPGDRFFWFTTTGWMMWNIVAASLLAG
                     ATALVYDGNPGYPDLGALWKFAEESGMTVFGTSASYLTSCMKAGVEPGRDVDLSALKA
                     IGSTGSPLPPEGFDWVYEHVKEDLWLFSTSGGTDLCTAFVGGVPLLPVRTGELQARSL
                     GAKVEAYDSNGEPQIEEVGELVITEPMPSMPIYFWNDEGGERYRESYFDMYPGVWRHG
                     DWIKIKENGSAVIYGRSDSTINRGGVRMGTAEIYSAVEKVDEVQDSLVVDVQTADKAF
                     MPLFVVLKEGVELDEDLKKRINRRIREDCSPRHVPNDIYAVPEIPKTLNGKKLEVPVK
                     KILSGTPPEDAASKDSLSNPEALDRFAELARNM"
     gene            12172..13581
                     /locus_tag="VFJ72_06055"
     CDS             12172..13581
                     /locus_tag="VFJ72_06055"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479061.1"
                     /translation="MASVSAGAQQAPERDITRVALTACAGSSIEWYDFFIYGTAAALV
                     FPALFFPESSALAGTLLSFSTFAVGFFARPVGGVIFGHFGDRVGRKKALVTALVMMGI
                     ATTLIGLMPTYATIGVLAPILLVLFRFIQGLAVGGQWGGAVLLATESAPRNRRGFYGS
                     FAQIGVPVGVILANLLFLLISSTLAPEAFQAWGWRIPFLMSIVLIGIGLYIQLRLEDT
                     PAFRHLQEYRESHQTEEEREEARLAAEAQRSPIIEAFRDYWKQILLAAGAFVSINANF
                     YIFITFIIAYGTNPDILGLPQTTMLAAVLIASVFQIPALLVFAGYSDRVGRRGIYMLG
                     AALLGAWAFVLWPLANTGSFILISLALVVGQAFLSMMYGPQAAFYSEIFTTRVRYSGA
                     SLGYQIGSIFGGALAPIIATALLAEFGSWVAIAVYQLFLSLVTLVCVFLLTETYQNEM
                     DEVRAATNRRTAGVQDPTA"
     gene            13655..14440
                     /locus_tag="VFJ72_06060"
     CDS             13655..14440
                     /locus_tag="VFJ72_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7479062.1"
                     /translation="MYETINLEKSGGVATIALNRPKVLNAFDGKMHEEIREALDAAAE
                     DDEVRCLVLRGEGRGFSAGADLAQIIEGSDGDPDLGEYLRETYSRLVTRMVSMEKPII
                     GALHGPVYGAGLGIALACDLRIAAESAKFSVAFIKIGLMPDAGVSFFLPRVVGLARAM
                     EMSMLGDPVEAEEAYRIGLVNRVVPDDALADESNALARQLAAMPTRALGRIKQSLYAS
                     FESDLETALEAEAQGQTLCGYTNDHKEGVAAFFEKREAKFTGS"
     gene            14452..16122
                     /locus_tag="VFJ72_06065"
     CDS             14452..16122
                     /locus_tag="VFJ72_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7479063.1"
                     /translation="MTESYFEFWPPRVPRSLPLPQTSVYHNLEVSASRYPEKTAIIYY
                     GTEIPYKRLLDEVNALAGYLQRELGVEKGDRVLLYMQNSPQFVMAFYAVLRLGAVVVP
                     VNPMLVTNELTHYVEDSEARVAIVGQELYPKVSPLLAESGSERVIVAAYSDYLEKETD
                     LAVPDVVTAPREEVTDEGVTSWGEALKSGHEPGPVVVGAEDMALLPYTSGTTGRPKGC
                     VHTHRTLQATLVGVGVWNTTTPAAVMLCVLPLFHVTGMQHSMNAPIYSGGTFVILTRW
                     DRELAAELIQRHGITHWTNISTMVVDFLANPKIGGYDLSSLVIVGGGGAPLPAAVGEK
                     LNDLTGLRYQEGYGLSETISQTHVNPPDRPKLQCLGVPHFDVDARVVNPATLEELGPN
                     EEGEIVVSGPQVMRGYWRRPDADEEAFFERDGKRFFRTGDIAVYDDEGYFFMVDRLKR
                     MINASGYKVWPSEVESILYKHPAIQEACIIGVPDEQRGETAKAVVVLREGESVSAEEI
                     VEWSKGQMAAYKYPRNVEFVEALPKSGSGKILWRELQEKEQQKMAEAK"
     gene            16141..>16323
                     /locus_tag="VFJ72_06070"
     CDS             16141..>16323
                     /locus_tag="VFJ72_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7479064.1"
                     /translation="MASMTAARFYEVNEPLRLEEVPVPEVQEDEVLLQMKAAGLCGSD
                     VHIVFEGVTPTAFKPIT"
BASE COUNT         2952 a         5073 c         5454 g         2844 t
ORIGIN      
        1 gtgctcggcc agccgccctc ggaggtcgag gaggtcctgg aggtcggcca cattaccgac
       61 ccggattcgg aggagatggt ccacgtccgg cggcgcctgc gggagttgct ggccggtgag
      121 gaaggggagg tcgagagtgc agtcttcgag tgaggcgcca tcccgcaagg tcccaacggc
      181 cgccgagagc ctgccgggca agtccaggag tcgccgtaaa ggctgggcgc ggctgccggt
      241 taccgtcagg tacgtgctgc tgctagcggt gttcgtcggg gcctggcaac tgtatgtcac
      301 gctcgcgcac gtaagcccgc tggcgctgcc gggaccgctg gacgtggcgc gggccttctg
      361 ggatggctgg gccagcggac ggctcgctga cgccaccctg acgacgctgc gggtgctcga
      421 gctcgggatg ctcgtcgggg tcattctggc ctccgtattt accgtcttcg cgacgtggac
      481 gaccgtcggc gcggacatcc tttcgctgct gacggcgatg ttcaacccgg tcccggcgat
      541 agcgatgctg ccgatcctga tcctgtggtt cggcatcaac tcgacggctc tcgcggtcgt
      601 gatcgtctac tccatcgtct ggcctatcgc catcaacctg agcgccggtt tcaagaccgt
      661 gaaccccacg atcatgatgg tcggccgcaa cctcgggctg cggggctgga gcatggtccg
      721 ggacgtgctg ctgcccgccg cgctgccgca taccatcacc ggcctgcgta ccggctgggc
      781 cttcggctgg cgcaccatag tggccgccga gctggtcttc ggcgtcgccg gcaagggcgg
      841 gggactgggg ttctttatca acgacgcccg ctacttcctg cggatactgg aggtcttcgc
      901 gggcatcctg accatcgccc tcatcggcat cctggtcgag gccgtcttca accgggtaga
      961 gcgccgcacc gtcgttcgct ggggcatgaa gtccgcggcg tagagcacac accatcgaag
     1021 ggaggacacc aagaatggac acgagaacgg ggagaatagc gagagagtac agccgtcagg
     1081 agttccttag tctggggatc gcggcggcgg gcgcggggct catgttcgcc ggttgcagcg
     1141 ccagcgacgc agcgggtggc ggcggtggct cggaggcgga taagctcagg atcacctacc
     1201 agcccggcac gaccacctac gcgcagctag tcatcatgga gcaggagggt tggctggccg
     1261 acgacctgca ggacgtggac atctcctgga accaggtgga cgccggggct gccgtgcgcg
     1321 acgccatcgc ctccggcaag acggacatag gggcgggcgg gatcgggcct ttcctcgtag
     1381 gctatgactc cggcatcgac tggcggatac tcagcgccct ggacaacgtg agcatgtggc
     1441 tgatggtcaa ggacgagaag ttcaagagcc tcaaggactt caaggagggc gacaagatcg
     1501 ccgtcgtcgc ccccgattcc atccaggccg tcatactgcg gaagggcgcc caggagcaac
     1561 tcggcgaccc gcacgcgctg gacgggaaca tgatctctct cccaagtccc gactcggtta
     1621 ccgcgctcct gaacggccag gtcgccgcgc acttcaccgc gccgccgttc cagtttcaag
     1681 agcaggacaa gggcgcgcgt cccatcctca agagcttcga cctcttcggg gagcacaatc
     1741 tcataagcct ctgggtcctg cgcgacttcc acgaggcgaa ccccaagatc atgtccgccg
     1801 tctacaagaa cgtccagaaa gccacgaagc tgatccagga cgacccgaat agagccgggg
     1861 agatccttgc caaagtctcc aagatagacc ccgccgaaga gacccgctac ctcacggaac
     1921 agggtgtctc atttaccacc acgccgcggg gcttcatatc gttcgcgaag ttcatgaaga
     1981 ccgccgatct cataaagaaa gtccccggct cctggaagga cctcgtctac gacaatatca
     2041 agaacagcga cggtagctga gcgctccgaa ataaaaagtc ctgccgaaag agtgatcctc
     2101 cggcaggact ggttttctag actgtttgct ctacgaggtt tcgaggaccg gcttgcgctc
     2161 gcggcgcgtg gtggagatcg ccaggtagag agccgaaacc cataccgcaa cgatggctat
     2221 gtagacgggg atgttgacgc tgggatcgaa cccgatggcg cccaggaagg tgttggcgtt
     2281 ggtcaggagg atgaacccac cgaccgcggt gcccatcacc ctcgcgttca ggtggcggac
     2341 cacccaggcc gctatcggcg cggcgatgat gccgccgacg aggatcgccc cgacgagctg
     2401 gaacggtatc tggttgaagt tgagggtgag caagaacccg gcgctggccg ccagagccac
     2461 gatgaactcg ctggtatcga cggtgccgac taccttgcgc ggctccatcc gccgcgagga
     2521 gaggagggtg ggtgtggaga tcggacccca accgccgccg ccgaccgcgt ccaggaagcc
     2581 cgccacgaag ccgaggggag cgagaaacgc tcgcgctatg ggccggtcgt tgatcaggtt
     2641 gcccgcccgc tcgaacgtga agcgcgccag gatgtagata cccaggagga agaggaacac
     2701 ggcgatgatc ggctccgcgg cctcggcgga gattaccgaa ctcagcacca ccgctcccag
     2761 gaacgcgccc accgcgcccg gtatggcgag ccagaatacc ttgctccagt ccacgttgcc
     2821 gaatcgccag tgcgagagcc ctgacgcggc cgtcgtgccc acctcggcta tgtggacgct
     2881 cgccgaggcg agggccggcg ctattcccgc cgccagcagc agggtggtcg aggttacgcc
     2941 gtaagacata cccagagccc cgtctaccaa ctgagcgatc aaaccagcta tacctaccaa
     3001 gagcagtcta gtcattcgtg ctaatccttc tagtgcctat atagtctact aatttagtgt
     3061 acttacttta caacaggcgg gactctacac ggtgtccctg tggttgtcaa gctgcaggaa
     3121 gcggtttgtt tcgaggttcg gtggcgtgtt ggatcggcga tgccgtagta gctacgcctt
     3181 tgccggcggg gcggggtctt cgagtcttat acggggctct ccactatctc gccgaacacc
     3241 ttggtgcggt ctatcttgac gtgtatatgt ttgccgcgct ccagttccag gtgccgggcg
     3301 tcttcgcgcg tcacctgcgc ccagaggctc tgtccgtcct tgagggttag ctcgacgcgg
     3361 acctcgaaac ccaggtagac gaggtggtcg accgtcgcct cggaggtcgt gccgttgggg
     3421 ctcagtttga tctcgatgtc gtgagggcgg acgtaggtgt tgcccagacg gttgaccggc
     3481 ccgatgaagc tcatgacgaa ctcgttggcg gggtgctcgt acaggtcttg cgggccgccg
     3541 acctgctcta tcttgccgtg gttcatcagg acgatacggt cggccacttc catcgcttct
     3601 tcttgatcgt gggtgaccag aatagtcgtc acgtggatgt cgtcgtgcag ccggcggagc
     3661 caggccctaa gctcctggcg cacccgggca tcgagcgcgc cgaaaggctc gtccaacagt
     3721 aggacctccg gttccacggc gagggcgcgc gccagggcca tccgctgtcg ctgtccgccc
     3781 gaaagctgcg tcgggtaacg gtgagagaaa ccgtcgagct gcaccagttc gagcaactcg
     3841 tgtaccttct tgtctatagc tcccctcgga tgcttgcgga tcttcaggcc gaaggcgacg
     3901 ttgtcgaaga cggtcatgtg cttgaaggcc gcgtagtgct ggaagacgaa gccaacctca
     3961 cgcttctgcg gcggcatatt cgtcttctcc tcgtcggaga taaagatctg gccgctatcc
     4021 ggcctctcca gaccggcgat ggctcgaaga agggtggatt taccgctgcc gctcgggcct
     4081 aaaagggcgg ttagagagcc ggtgggcgcc tccaccgata cgtcgtcgag cgcgacgaaa
     4141 tcaccatatc ttttagagac gttgcgcgct gtgattccca ttagtattcc tccttggggc
     4201 tggtcaggtt cgtcaagagc agcgtcgcca cggcgatcac ggcgaggacc accgcggcgg
     4261 tgtaacagcc gacgaggtcg aagctctgga agcggtcctc gacgtagatg gtgagcgtct
     4321 ccgtacggcc ggcgagcttg cccgagacga ggctgaccgc cccgaactct cctatggccc
     4381 gggccgtcgt caggacgatg ccgtaggcca cgcccgagcg gatcgccggc agcgtaacca
     4441 gccggaacgt ctggaacgcg gaggccccga gcgtggaggc ggcctgttcc tgctcggtgc
     4501 cgatctcgcg cagtacgggc accacctcgc gcacaacgaa aggcagcgag acgaagatcg
     4561 tggcgaggat tatccccggc gtggagaaca cgatccttat ccccgcctcc gcgagccatc
     4621 cccccacaag gctggtacgg ccgtagatga tcaggagcgc gagcccgacg acgaccggcg
     4681 agacggcgag cggcaggtcg agtacggcgt tcaggagcgc cttgccgcgg aactcgtgcc
     4741 gcacgagcac cagcgcgcag acgatgccga agaccgtgtt ggcggttacg gcgatcaccg
     4801 ctaccagcaa cgtcagccac agggcgtgca gggcctcggg gcgggtgacg gcctcccaga
     4861 ccgctccaag accgttgctg aaggtacgca cgaagaccat gctcacgggc aggacgagca
     4921 ggagcgccag gtatcccaga gccagagtgc ggagcgtgat cctgctagcg gtcatggcgc
     4981 gttccccggc gattgaccag gctcattccg aacaggacga gcagcgagac gacaagaagc
     5041 accacggaga cagcggcggc gcccgccgtg ttcccgctct ccacctgacc gaagatatac
     5101 actgaggcga cctgggtttt gaacggctgg tttcccgtta taagaaccac cgagccgaac
     5161 tcgcccacgg agcgggcgaa gcccagagcc gcaccggaga ggatagcggg catcaggttg
     5221 ggcaggatga tgcggcgaaa ggtcacgaac ccgctcgccc cgagcgaggc cgccgcctcc
     5281 tccatgtcgc ggtctatctc catgagcacc ggctgcacgg cccggacgac gaacggcagt
     5341 gtgacgaaga gtagcgccat cccgaccgat accttggtgt aggcgacgtt caggcccagc
     5401 ggactcctcg gtccatagag cgccaggagc gtcaggccgg ctacgatggt cggcagggcg
     5461 aaaggtaagt ctatgatcga gttcaccagc cccttgcccc ggaaggagtc tcgcaccagc
     5521 acccaggcga tcagggttcc cataacgacg ttaatggcga cgacggccag cgaggcgaag
     5581 aaagtgagtc ttagagcggc gagagcctgc gggttggtta tcgcccgtac gaagccctcg
     5641 atgccgccct ccgtggacct ccacacgacc gccgccagag gtatgaggac cagcaggctc
     5701 aggtacccga ccgcggcccc gcgggtaaga gcgctgccct ctcgcttgga tgctcgggag
     5761 cccgcagcgg gcgctgtctg cgtgttgctc gttacgctca ttcggtgggc gcgcctactt
     5821 cgcggttgat cttcgccatg acgcccttgt ccggatcgaa gaacttgtcg ttgaccttat
     5881 cccagccgcc caggtccttg atcgtgaaca gaccggaagg ctcgggataa tcgaactcgc
     5941 cgagtacctt cttgtccacc ggacgatagc ccgtgttgcc gaaggtgcgc tgggccttct
     6001 ccgtgtacag gaacttgacg aacgccttgg cctgatccgg agcctggctg tcgctcgtca
     6061 ccgcgacggg gttctctatt agtatcgtct ggtccggcac gacgaagtcg agatcctcgc
     6121 cttccttctg ggccaggatg gcttcgttct cgtaggcgag catggcgtcg cccttgccgc
     6181 cggtgaaggt ggcgagggcc tcgcgggcgc tcttatcctg caccgggacg ttgtggaaga
     6241 gcttgctcag gtagtcgatc ccctcctgat ctgacttgcc ctgcttgatc tgggctccgt
     6301 aaccggccat gatgttccac ttcgcgccgc cggaggtgaa cgggttaggc gtgatgacct
     6361 cgacgccggg tttgatcagg tcgtcccagt ccttgatgcc tttcgggttg cccttgcgaa
     6421 cggcgatgac gacgaccgag tcggtgacca tgccgtcgta ctcgttcttg ttccagttgg
     6481 aatctacgag acccgccttg accaatcggt ccatgtccgg cgcgagcgag aactccacca
     6541 cgtccgccgg cagaccaccc tccacagcgc ggctctgctc accagaactg ccgtaggaag
     6601 tgtcgaactt gacgcccttg cccgcgtcgg tcttctggaa cgcgggtatg agcttgtcat
     6661 acgcctcacg cggggtcgag taagccacga tcgtgaggtt gcctccgccg ccaccaccct
     6721 gcgcgctgga tgacccacct ccgcatcccg ccacggccaa cagagctatc agagccacca
     6781 atataccgag gaaccttgta acccgtatcg acctcatatg cccgtccatt attctcctct
     6841 aaagctattt tgtctactca atttatgaac aacgtggaac cctagatcaa agggcgcagg
     6901 gtgtcaagtg ttggcgggca ctaattttgg ggcgtcgggt ccggggttgg tgtacctttg
     6961 ggttcaggga ccgaaggaag ggagccggag tgaagcaggt ggagttctac tacgacctcg
     7021 tcagcccgta ctcgtacctg gcttacggga ggctgcgcga gatctgtgct gagcagggag
     7081 ccgagttgaa gctgcgtccg atgcttctcg gcgcggtcca caaggcgacc ggaaaccagg
     7141 ccccgataga ggtcgaggca aagggacgtt accagtaccg ggacatccac cgctgggcgg
     7201 actactacgg tctgtcgctg cggttccccg acccgttccc tttccgcacc ctgaagacca
     7261 tgcgggcggc gatgttctgc gaggcgcgcg gggagctgga ggctttcacc cgcgaagggt
     7321 tcgccctcta ctgggaggaa ggcggagcgc cgaagagtct cgcggaagcc gacgaggacg
     7381 gtcccgtctc ggagatatcg cgccgcatcg gcgccgactc ggaggaggtg ctggagggcg
     7441 catccgggca ggaggccaag agcgccctca aggaggcgac gaccgcggcc gtggagcgcg
     7501 gcgtcttcgg ggcgccggcg ttcttcgtgg gcgaggagat gttctggggt aacgaccggc
     7561 tgcacttcgt cgaggcggca ctcggagaga gccgggagac agtcgcgaga tgacgccgtg
     7621 agagagggag actgagagag ccattcatgt tacctttcag gcattgggga atgcgatctc
     7681 cgcgtccggg tgaggcgcgg ggaaggatcg gaggagagac atggaaagat taggtgggga
     7741 gcagatagga cagacgtcgt ccatccgtca ggtagcgctc gccagcttta tcgggacggc
     7801 catcgaatgg tacgacttct tcctctacgg gacggcggcg gcgctgatct tcggcaagtt
     7861 gttcttcccg gagttcagct cgaccgccgg gactctggcc gcgttctcga cgtatgcggt
     7921 cggtttcgcg gcgcgtcccc tgggaggcat agtcttcggg cacttcgggg acaggatcgg
     7981 acgcaagtcc atgcttatat tgtcgctcat gataatgggt gtagcgacgg ccctgatcgg
     8041 tctgttgccg accttcgagt ccatcggcgt cctggcgccg atactgctcg tcgtgctgcg
     8101 cttctgccag gggatcggcg tcggcggcga gtggggcgga gcggtcctga tggcggtcga
     8161 gcacgcgccg caaggccggc gcggcttcta cggtagctgg ccgcagatgg gcgtcccggc
     8221 ggggcttctc acggggaacc tcgtcttcct cgcggcgacg gcggtcttgc cggaggcgtg
     8281 gggctggcgg gtgccgttcc tgttcagctt cgtgctcgtc ggggtcggcc tctttatccg
     8341 gctcaggata ctggagagcc cggccttcac tcgggtcaag gagtccggca ccgaggctcc
     8401 catgccgatc ctggacgtgc tccgcaccta cccgaagaac gtcctgctcg cgatggggat
     8461 gcgcttcgcg gagaacggaa ccttctacgt cctgaccgtc ttcgtgctca cctatgtcgt
     8521 ggaagaactc aagctggagc agacgacggg acttatcggg gtcctcatcg ccgcctttat
     8581 cgggctattt accgtcccgt tcttcggaca tctctccgac cgcgtggggc ggcgtccgct
     8641 ctacttgctg gggtcggggt tctcgctgct cttcgccttc ccgttcttct ggatgctcaa
     8701 cacggggagc gcgccactga tctggctcgc catcatcctc ggcgtgaacg tcggccacga
     8761 cgccatgtac ggaccgcagg cgtccttctt cgccgagctg ttcgggacgc gggtgcgcta
     8821 ctccggcgca tcgctcggat accagctcgc ctcggtcttc gccggagggc tcgcgccgct
     8881 catcgccctc tacctgctca ccaccttcgg gtacggcgcg gtcgcggcct ataccgccct
     8941 aatggccctc ataaccgtaa tctccgtcat tctcgtcacc gagacctatc agagcgacat
     9001 ccatcagacc caggccgagg agcggcgttt gatctccgac cgggcagagc ccaggactca
     9061 atagaccatt atccgccggg cgccattctc tgagtggcgc ccggcgatta ggaaggtgaa
     9121 cttccaaccc catccagacc gcggcatgcc ctatactgat ccatacaggt tgggaaggct
     9181 cctatcgagg aggaagaacg catgagcgag gcatacgtca ggccgggaca gtcggccgtg
     9241 agcgtggacc tgacagggaa gagcgccctg gtgaccgggg ccgcgagcgg catcgggaag
     9301 gcgtgcgccg aacggctggc gaacgcgggc gcggaggtca ccgtgctgga ccttaacggc
     9361 gacgccgccc gggaggtcgc ggacagcatc ggcggacagt cggtgcaggc cgaccttgcc
     9421 gactacgagg tggtggattc gctggacatc gaggcggaca tcgtggtcaa caacgccggg
     9481 cttcagcacg tcgcggccat agaggacttc ccgccggagc ggtactccat gatcctgcgg
     9541 ataatgctcg aagcgccgtt caggatcgtc cacaagacgc tgccggggat gtatgagaag
     9601 ggctggggcc gggtcgtcaa catctcctcg gtgcatggcc tgcgcgcctc cccgttcaag
     9661 tcggcctaca tctccgcgaa gcacgggctc gaaggtctct cgaaggtcat cgcgctggag
     9721 ggcgggcctc acggggtcac gtccaactgc atctgcccgg cctacgtgcg gacgccgctg
     9781 gtggagggac agatcgccga ccaggcccgc acgcgcggta tctccgagag cgaggtcata
     9841 gagaaggtga tgctgacgag cccggccatc aaacgcctcg tcgagccgga ggaagtggcc
     9901 gagctggtgg cgttcctgtg cacgccggaa gcctcgttca tcagcggcgc atccctgacc
     9961 atagacggcg cggcgacggc ccagtggtag cccggagcgc gggaagatca cggaggagga
    10021 actagcatga ccgaaacggt cgagggccgg ctactctggg agccctccga agagttcaag
    10081 cagaacgcca acgtcactcg ctacatggaa tggctgcgcg acgagaagga ccatgacttc
    10141 gcggactacg ccgcgctctg ggaatggtcc gtcacggacc tggaaggctt ctgggaatcc
    10201 gtgtgggaat attgcggcgt aaaggccgcg aagccgtact ccgccgtcct gaaagaccgc
    10261 aagatgcccg gcgcggagtg gttcgccggg gccgagttga actacgcgga gcacgccttc
    10321 ctcaaggcgc gggatgatga gcccgcgatc atctcccagt ccgagatccg tccgatgtcc
    10381 gagatgagct ggggcgagct gcgcgcgaag accgccgcgc tcgccgctgg gctcagggag
    10441 atgggcgtcg agcgcgggga ccgggtggtc gcctacatcc cgaacgtgcc ggaggcgctg
    10501 gtggccctgc tcgcctgcgc ctctctgggc gcggtctggt ctagctgctc cccggacttc
    10561 ggtgtcggga gcgtcgtgga ccgcttcaag cagatagagc cgaaggtgct gctcgcggta
    10621 gacggctacc gctacggtgg caaggactac gaccggacgg acgtggtggc gaggcttcag
    10681 cgcgagatac cgagcctcca gaagaccatc gtcttaccgt acctgaacga cgacccggac
    10741 acgagcggcc tggaggacgt ggtcctctgg gacgaggtgc tcgcctccaa cgagggggcg
    10801 gagttgacgt tcgagccggt tccattcgac catccgctct gggtgctcta ctcctcgggg
    10861 acgacggggc ttccgaaggc gatagtccag agccagggcg gcatcctgct ggagcacctc
    10921 aaggcgctca tcctgcacat ggacctcgcg cccggcgacc gcttcttctg gttcaccacg
    10981 acgggctgga tgatgtggaa catcgtggcc gcgagcctgc tcgccggagc gaccgcgctc
    11041 gtctacgacg gcaaccccgg ctacccggac ttgggcgctc tctggaagtt cgccgaggag
    11101 tccggcatga ccgtcttcgg gaccagcgcc agttacctca cctcgtgcat gaaagccggc
    11161 gtggagccgg gccgggacgt ggacctctcc gcgctcaagg cgataggctc gaccggctcg
    11221 ccgctgccgc ccgaaggctt cgactgggtc tacgagcacg tcaaggagga cctctggctc
    11281 ttctcgacca gcggcggcac ggacctgtgc accgctttcg tgggcggggt tccgctcctg
    11341 ccggtccgca cgggcgagct tcaggcgcgg tcgctcgggg cgaaggtgga ggcgtacgac
    11401 tcgaacgggg agccccagat agaggaagtc ggggagctgg tgatcacgga gccgatgccc
    11461 tcgatgccga tctacttctg gaacgacgag ggtggggagc gctaccggga gagctacttc
    11521 gacatgtacc ccggcgtgtg gcggcacggg gactggatca agataaaaga gaacggttcc
    11581 gccgtcatct acggccgctc cgactcgacc ataaaccgcg gcggcgtcag gatggggacc
    11641 gccgaaatat actccgccgt cgagaaggtg gatgaggtcc aggacagcct ggtcgtggac
    11701 gtgcagaccg cggacaaggc cttcatgccg ctcttcgtgg tgctcaagga gggcgtggag
    11761 cttgacgaag acctcaagaa gcgcataaac aggcgcatcc gggaggactg ctcgccccgg
    11821 cacgtcccga acgacatcta cgccgtgccc gagatcccga agaccctcaa cggcaagaag
    11881 ctggaggtgc cggtcaagaa gatcctctcc ggcaccccgc ccgaggacgc cgccagcaag
    11941 gactccctga gcaaccccga agccctggac cgcttcgcgg agctggcgcg gaatatgtag
    12001 gaatggaggt ccggagaacg aggcttgact ggaagccaaa aacggtttag gtttgcgaca
    12061 ggaggctggg gaaggagcct tgcatagctc gatctacaat gagttaaaga gagttcgggg
    12121 aagactctga agggaaacgc gactcgaaaa taggggtgat gggagggatg tatggctagc
    12181 gtgagcgccg gagcgcagca ggctccggag agagacataa ccagggtagc cttgacggcc
    12241 tgcgcggggt cgagcatcga gtggtacgac ttctttatct acggcacggc ggcggcgctg
    12301 gtcttcccgg cgctcttctt cccggaatcg agcgcgctcg ccgggacctt gctctcgttc
    12361 tcgacgttcg cggtcgggtt cttcgcccgg cccgttggcg gtgtgatctt cgggcacttc
    12421 ggcgacaggg tggggcgcaa gaaggcgctc gtgaccgcgc tggtgatgat ggggatagct
    12481 accacgctta tcggcctcat gcctacttat gcgaccatcg gcgtgctggc gccgatactc
    12541 ctggtattgt tccgctttat acagggcctc gccgtgggcg ggcagtgggg cggagcggtc
    12601 ttgctggcga cggagagcgc gcccaggaac cggcgcggtt tctacggaag cttcgcccag
    12661 ataggcgtcc ccgtcggggt catcctcgcc aacctgctgt tcctgctcat cagctccacc
    12721 ttggcccccg aggcgttcca ggcgtggggc tggcggatac cgttcctgat gagcatagtc
    12781 cttataggca tcgggctcta catccagctc cggctggagg acactccggc tttccggcac
    12841 ctgcaggagt accgcgagtc gcaccagacc gaggaggagc gggaggaggc ccgccttgcg
    12901 gccgaggccc agcggtcgcc gatcatcgag gcgttccggg actactggaa gcagatcctc
    12961 ctggccgccg gcgcgttcgt ctcgatcaac gcgaacttct acatcttcat tacgttcatc
    13021 atcgcctacg gcactaaccc ggatatcctc gggctgccgc agaccacgat gctcgcggcg
    13081 gtacttatcg cctcggtctt ccagataccg gccctgctgg tgttcgccgg ttactcggac
    13141 cgggtggggc ggcgcgggat ctacatgctc ggcgcggctc tgctgggagc ctgggcgttc
    13201 gtcctgtggc cgctggccaa taccgggtcg ttcatcctga tctcgctcgc gctggttgtc
    13261 ggacaggcgt tcctgagcat gatgtacgga ccgcaggcgg cattctactc ggagatcttc
    13321 acgacgcgcg tacgctactc gggagcctcg ctcggatacc agatcggctc catcttcggc
    13381 ggcgcgctcg ccccgataat cgccaccgcc ctgctcgcgg agttcgggtc ctgggtcgcg
    13441 atagccgtct accagctctt cctgtccctc gtaaccctgg tctgcgtctt cctgctgacg
    13501 gagacctacc agaacgagat ggacgaggtc cgcgccgcca cgaaccgcag gaccgccgga
    13561 gtgcaagacc cgacggccta gaggacgaaa ccttacgaga tgcctcccgc tagcaggacg
    13621 cgggaggcat ctcgccgacg gaaggagttt cactttgtac gagaccatca acttagagaa
    13681 gagcggcggc gtcgccacca tcgccctgaa ccgtccgaag gtgctcaacg ccttcgacgg
    13741 aaagatgcac gaggagatcc gcgaggcgct ggacgccgcc gcggaggacg acgaggtgcg
    13801 ctgcctggtc ctccggggcg agggacgggg cttctccgcc ggggcggacc tggcccagat
    13861 catcgagggc agcgacggag acccggacct cggagagtat ctgagagaga cctacagccg
    13921 gctcgtcacc cgtatggtct cgatggagaa acccattatc ggggcgctgc acgggccggt
    13981 ctacggggcg gggctcggga tcgcgctggc ctgcgacctc cggatcgccg ccgagagcgc
    14041 gaagttctcc gtggcgttca tcaagatcgg tctgatgccc gacgccggag tctcgttctt
    14101 cctgccgcgc gtcgtcggtc tcgctcgcgc gatggagatg agtatgctcg gagacccggt
    14161 cgaggcggaa gaagcgtacc gcatcggtct cgtgaacagg gtcgtccccg acgacgccct
    14221 cgcggacgag tcaaacgcgc tcgcgcggca gctggccgcg atgccgaccc gcgcgctcgg
    14281 gaggataaag cagagcctgt acgcgagctt cgagagcgac ctggagacgg cgctcgaagc
    14341 cgaggcccag gggcagacgc tctgcggcta tacgaacgac cacaaggagg gcgtcgccgc
    14401 cttcttcgag aagcgcgagg cgaagttcac cggcagttag gaggcgccga gatgacagag
    14461 agctacttcg agttctggcc gccgcgggtg ccgcgctcgc tgccgctgcc gcagaccagc
    14521 gtctaccaca acctggaggt gtcggcgagc aggtacccgg agaagacggc catcatctac
    14581 tacggaaccg agatccctta caagaggctg ctcgacgagg tgaacgccct cgccggatac
    14641 ctgcaacgag agctcggcgt cgagaagggc gaccgggtgc tgctctacat gcagaacagc
    14701 ccgcagttcg tcatggcgtt ctacgcggtg ctgcgcctcg gggccgtagt cgtgcccgtc
    14761 aacccgatgc tcgtcaccaa cgagctgacg cactacgtcg aggactcgga ggcgcgggtc
    14821 gccatcgtcg gacaggagct gtacccgaag gtctcgccgc tcctcgccga gagcggcagc
    14881 gagcgcgtga tagtcgccgc ctactcggac tatttggaga aagagacgga tctcgccgtc
    14941 ccggacgtgg tgacggctcc gcgcgaggag gtaactgacg aaggtgtgac ttcctggggc
    15001 gaggcgctaa agagcggcca cgaacccgga cccgtggtag tcggcgcgga agacatggcg
    15061 ctcctgccgt acacgtcggg gacgacgggc cggcccaagg ggtgcgtcca cacgcaccgg
    15121 acgctccagg cgacgctcgt cggggtgggg gtatggaaca cgacgacgcc agccgccgtg
    15181 atgctgtgcg tgttgccgct cttccacgtg accgggatgc agcacagcat gaacgccccg
    15241 atctactccg gcggcacctt cgtcatcctg acccgctggg accgggagct cgccgccgaa
    15301 ctcatacagc gccacggcat cacccactgg acgaacatct ccacgatggt cgttgacttc
    15361 ctggctaacc cgaaaatagg cggctacgac ctctcctcgc tcgtcatcgt cgggggcggc
    15421 ggcgctccac ttccggcggc ggtcggcgag aagctgaacg acctgacggg gctgcgctac
    15481 caggagggct acgggctctc ggaaaccatc tcccagaccc acgtcaatcc gccggaccgg
    15541 cccaagctcc agtgcttagg tgtcccgcac ttcgacgttg atgcgcgcgt cgtaaacccc
    15601 gcgacgctgg aggagttggg tccgaacgag gagggagaga tcgtggtctc cggcccgcag
    15661 gtgatgcgcg gctactggag acgccccgac gccgacgagg aggcgttctt cgagcgcgac
    15721 ggcaagcggt tcttcaggac gggcgacatc gcggtctacg acgacgaggg ctacttcttc
    15781 atggtggacc ggctcaagcg catgatcaac gcctccggtt acaaggtgtg gccttccgag
    15841 gtcgagtcca tcctctacaa acacccggcc atccaggagg cgtgtatcat cggcgtcccg
    15901 gacgagcagc gcggagagac ggcgaaggcc gtggtcgtgt tgcgggaagg ggagagcgtc
    15961 tcggccgagg agatcgtcga gtggtcgaag gggcagatgg ccgcctacaa gtacccgcgg
    16021 aacgtggagt tcgtggaggc gctgcccaag agcgggtccg gcaagatcct gtggcgcgag
    16081 cttcaggaga aggagcagca gaagatggcg gaggcgaagt agctaccgga aggagtgcgc
    16141 atggctagca tgacggcggc ccgtttctac gaggtcaacg agccgctgcg gctggaggag
    16201 gtccccgtgc cggaggtaca ggaggacgag gtcctgctcc agatgaaggc ggccgggctc
    16261 tgcggctcgg acgttcacat cgtcttcgag ggcgtgacgc cgacggcgtt caagccgatc
    16321 acg
//
LOCUS       DASSMB010000094        23877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_399733, whole genome shotgun sequence.
ACCESSION   DASSMB010000094 DASSMB010000000
VERSION     DASSMB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 23877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23877
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399733"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="VFJ72_06075"
     CDS             <1..824
                     /locus_tag="VFJ72_06075"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7479065.1"
                     /translation="EAYGPFTNEKVVGEALAPFRDEVVIATKFGFKDGDPQAGLDSRP
                     ERIRQVAEESLRRLKTDRIDLFYQHRVDPDVPIEDVAGTVKDLIQEGKVEHFGLSEAG
                     VQTVRRAHAVQPVTALQSEYSLWWREPEEEVLPTLEELGIGFVPFSPLGKGFLTGKID
                     ENTTFDSSDFRNIVPRFTAEARRANQALVDLLGEIAQRKNATPAQIALAWLLAQKLWI
                     VPIPGTRKLERLEENIGAVTVELTSSDLREIESAASKITVQGARYPEELERRIDR"
     gene            1073..2425
                     /locus_tag="VFJ72_06080"
     CDS             1073..2425
                     /locus_tag="VFJ72_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479066.1"
                     /translation="MENIEARLGGEDIHPPTSIRKVALASFIGSTVEWYDFFVYGTAA
                     ALVFGQLFFPESTPLVGTLSAFATFAIGFLFRPLGGAFFGHFGDRVGRKSMLVITLLV
                     MGTSTVLIGCLPTYNSIGILAPILLVALRAIQGFSVGGEWAGGSLMVVEHAPPGRRGF
                     YGSWPQVGPSAGTLLSAGVFAIFSALPEDQFLAWGWRMPFLLSAVVVIVGLVIRLRLA
                     ESPVFEQVKETHTGARLPVLDVFRTNAKNIFLIAGMRLAINTTFYASTVFALSYATEQ
                     LGIPRSTMLTGILITAALGFVSKPIYGALSDRIGRRPIYAAGSIIGALMAFPFFFAVE
                     SKSAILIFLAFFLIINISHDLNDAVESSFFSELFGTRVRYSGAALGHQLGAAVTGFTP
                     LIAAALAAAGGWTYVAVYVMFACLVSAGCVFMAAETFRKDIAEVDPLERQIMTEPRVR
                     "
     gene            2538..2960
                     /locus_tag="VFJ72_06085"
     CDS             2538..2960
                     /locus_tag="VFJ72_06085"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET7479067.1"
                     /translation="MFVLNGVNLGALGTRRPEVYGTLTLGDIEQRLRESFPEVDFEFR
                     QTDYEGEMVGYVREARGSDGIVINPGAWTHYAYALHDALEAVEGVIKVETHLSNVHAR
                     EPWRRHSVISPAVNAVVAGMGPYGYEAAVGYILRQLKA"
     gene            3024..4052
                     /locus_tag="VFJ72_06090"
     CDS             3024..4052
                     /locus_tag="VFJ72_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7479068.1"
                     /translation="MKAFVKTGHQPGEAGVRDVSVASPGLGEVLLRVASCGVCGSDIH
                     AFRSDAGFEWVRAPVTLGHEFSGTVEEVGPDVTRVREGDQVVAVAIQGCGRCEACLAG
                     STQLCQDRVAVGLSRDGGMAEYAVMPEQHLVPVPEGLDLTVAALGEPLSVAVHAVNVR
                     ADIKPGQNVVVSGPGPIGILCGMLARLRGAQVLLTGVGNDSESRLPAAERVGLRTANL
                     SDKPLEEHLRDSFGEPAPDVWIESSGSVRALDSALESVRPGGTVVVVGIYAEEMRFSP
                     TASVRRELSLLFSYSCNYADYEEALDLLGSGAIDPGPLLSKYPLQDASEAFEAVGKGR
                     AVKAILVP"
     gene            4076..4936
                     /locus_tag="VFJ72_06095"
     CDS             4076..4936
                     /locus_tag="VFJ72_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7479069.1"
                     /translation="MNTVPEVGIDHLTMLDVPPPDLVTIAHEAGFDSISPRVAASTPS
                     EDPWPMTPGSLMLEETARRLDETGTRILAVEVVRIGPDTKREDYEGALEAGARLGARY
                     VTVNSDDPDLDRASETFAALTGAASPYGLRPIIEPIIYTQVSNLDEAIYIAERSGGGG
                     VLLDTLHFQRYGGDLGQLRSLDPDLLSYVQLCDAPLAPPTGLPRPLKLPRGQSTDGTD
                     LQIESRAMRMLPGEGELPLAEFLAALPGEIPVSVEAPVMSLRETLTPIEFARRAREAV
                     ANVLSATRAS"
     gene            4947..6341
                     /locus_tag="VFJ72_06100"
     CDS             4947..6341
                     /locus_tag="VFJ72_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7479070.1"
                     /translation="MNVDHPLLPERVDALVVGGGMAGLAAAMVAAENGARVLVVEKGP
                     RPGGSAALSVGMFWTVPDFDTLRRRVPLGDPDLGRAVVEDYPAAIETIREMGVSVGER
                     VSGVMTVGIGHSIDVHALLEHEVAEIERSGGRVACDTTVRHLLTDDEGAVTGAVVRGP
                     GGQSAILADATILATGGFQGDPGLLSTYMGPNSDRMLVRSNRGSVGDGLRLGRDAGGA
                     TSRAMSSFYGHLVPSPLSSFTEDQYMPLAQYHSNHCILVNRLGRRFCDESQGDEVSVQ
                     ALLAQPEARGVLLCDERVRREYVVSEPFPNAGLLDRFERAAEAGGRLTSSDTLDGLVG
                     QVAGWGIDAVQLRATLESYAAAARREPVSLDAPLPTGPRPLVEPPFYALEVQPAITFT
                     YGGLRTDSDGRVLDHDGVPVGGLWAAGVDAGGLSNWTYAGGLAPAFITGRRAASSALA
                     ALDPEARQDPARPL"
     gene            6385..7218
                     /locus_tag="VFJ72_06105"
     CDS             6385..7218
                     /locus_tag="VFJ72_06105"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7479071.1"
                     /translation="MISGKTRLLALIGHPVSHSLSPAMHNAAFAADGLDYVYVCLDVD
                     PADLPAAVEGAAALKLRGFNITMPHKRAMIPLVDELDEGARVSGAVNTVVIEDSTLRG
                     YNTDGGGMVMACREAGIQLSGRSVLILGAGGAAAAIAVAFGEAGIGELHIANRSVEHA
                     AELREKLRGAGMEKVTIYSLDALDQAFSEAEIVVNTTPLGMKEADPLPIPPEYVLKDR
                     AFCDAVYRPGAETPLVRLARERGATVVAGGRMLLYQGVLAQKLWTGHEPNVKVMDAAL
                     S"
     gene            complement(7232..7708)
                     /locus_tag="VFJ72_06110"
     CDS             complement(7232..7708)
                     /locus_tag="VFJ72_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7479072.1"
                     /translation="MFPTRILLATDGTWEAALAEETAVELSDGTGSELHVVHVVHLTP
                     ELPYPNTALRERSEALLEWKKLDGIRLLDDRVRRLEELGCSVTASYYREGWPQKEVIR
                     LAEEIDAGLVITGGHRRPWFERIFCGPGFSETVLRRAGRPVLVVSERRLQGSTVPR"
     gene            complement(7874..8491)
                     /locus_tag="VFJ72_06115"
     CDS             complement(7874..8491)
                     /locus_tag="VFJ72_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479073.1"
                     /translation="MDEWHGFGARLSVSQIYETTVMDGQSGLSAHFVVVPSRSLGGDS
                     YLFRAPNGPLEALGLQALADVPAGGVVRSLAACLVSGGVDEFLQRFHEGFAQEVQHAS
                     TELPAAFAFAEYITFARVIPFEWTAFSADSLGNVLTAQGRGEAAFASYEETQAPVMVI
                     AIPAGVLICGSTANLEDALEAGLRRRILALVQSNSGTSVQGEESP"
     gene            complement(8549..9751)
                     /locus_tag="VFJ72_06120"
     CDS             complement(8549..9751)
                     /locus_tag="VFJ72_06120"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET7479074.1"
                     /translation="MSNPIQDGMAEATRLTREGKLAEATALIQRALGGGFTGGSTGAT
                     DTSSNFGDPLQAILHAINEDPRSATASQQDQDTQTLRPAPRHARPHRTSRGMPRSPGT
                     LTVEPLGTPTEPGRFIERSYTNRFGTRAYKLYIPTGYVGQAVPLVVMLHGCTQNPNDF
                     ASGTGMNTLAEEHTFLVAYPAQSGNANMQKCWNWFQAADQQRGQGEPSLIAGITCEVV
                     GEYHVAADRVYVAGMSAGGAMAAVMGATYPDVYAAVGVHSGLAPGAAHDMSSAFTAMR
                     NGMHAGKAGAMAPTIVFHGDRDTTVHPRNAEHLISHGLKGTPGAQAAQSQGQSPGGRS
                     YTRVSYEDAEGRTVVEQWTVHGLGHAWSGGDSRGSYTDPTGPDASAEMMRFFEQHSLQ
                     GAGDASDG"
     gene            complement(9769..10167)
                     /locus_tag="VFJ72_06125"
     CDS             complement(9769..10167)
                     /locus_tag="VFJ72_06125"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479075.1"
                     /translation="MSTSELEKITINLGMVDLGQIDLLVQEGFYANRTDFIRTAIRNQ
                     ISTHAETVRQTVARKTLVLGLQHYTRRDLEAVRDAGEMLEIKVLGLASIADDVSPELA
                     LETIESIVVLGSFRASPAVKAALADRIRPS"
     gene            10486..10668
                     /locus_tag="VFJ72_06130"
     CDS             10486..10668
                     /locus_tag="VFJ72_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479076.1"
                     /translation="MKIRQANGIGRVHVAKPQMKETYCGRPVDEEEWVTTSREANCVA
                     CARAGAPRPEGSGRGE"
     gene            complement(10769..10984)
                     /locus_tag="VFJ72_06135"
     CDS             complement(10769..10984)
                     /locus_tag="VFJ72_06135"
                     /inference="COORDINATES: protein motif:HMM:NF022608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HET7479077.1"
                     /translation="MAHEFKRGDKVEWNTPQGKTRGKVKKKLTADTQVGGTKISASED
                     DPRYLVESQKSGREAAHKPDSLSLVKE"
     gene            complement(11121..11786)
                     /locus_tag="VFJ72_06140"
     CDS             complement(11121..11786)
                     /locus_tag="VFJ72_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET7479078.1"
                     /translation="MLKRSAVLAAMLMALLAAPVPVMAQDAEALSGGTADGKFRLTLD
                     GWAPENVSFWVETSSGAGGVICTTDAAMVETGIPECRDGGSTNEMAFSAPAGSTLDYR
                     IIGSQGSELSQEVVAEGSKVADTGFIVDASYTFSGDTIGGDPAGTHPDGNASADNNDD
                     GAASGQYEDDSSSDGGPSSENVDSNAAVSVLPDTGGASVAVIAGAALLLAAGGFLAYR
                     TIS"
     gene            12142..12606
                     /locus_tag="VFJ72_06145"
     CDS             12142..12606
                     /locus_tag="VFJ72_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7479079.1"
                     /translation="MQVSAKTDYALRAAAELARAAADEAGPMKGEFISVSQGIPKKFM
                     ENILHDLKRAGIVRTQRGASGGYWLALPADKVSVADVIRAVEGPLANVRGEWPEAVEY
                     TGAAEHLQEVWIAVRANLRAVLETVTLADIVEGKLPTPVTKLTRDPEAWVHH"
     gene            complement(12613..13647)
                     /locus_tag="VFJ72_06150"
     CDS             complement(12613..13647)
                     /locus_tag="VFJ72_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HET7479080.1"
                     /translation="MPPELFVDLGKNAEMRWEAMSGRGYLTEIDRFYVRNHGPTPTID
                     AATWTLRVEGPGVERALKIGYEELLTLPSASVVCNLECAGNGRVFFEEVQGRGAEGAL
                     WRLGAIGVAEWTGVPLGELLSLAGLKPSAVEVMPEGLDEERGRRPMPVGKALEMDTIV
                     AYAMNGEPLSADHGFPSRVVVPGWYAVASIKWVGRIYVSEEPVHVPWNTEEYVLAGPG
                     YEARNPGLGPAVTAQGVKSALELPWPARLATGRRGIQGRAWSGAGEISRVDFSLDGGA
                     SWNPARLTGQNLPRAWACFRFEWEPEPGEYEIRIRATDIAGNTQPEAAPHNEQGYLYN
                     AVVAHPVTVY"
     gene            13771..15054
                     /gene="chrA"
                     /locus_tag="VFJ72_06155"
     CDS             13771..15054
                     /gene="chrA"
                     /locus_tag="VFJ72_06155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HET7479081.1"
                     /translation="MARKEVGFGEALRFWVKLGFINFGGPAGQISIMHRELVERKRWI
                     SESQFLRSLNFCMLLPGPEAQQLATYIGWRMHGVLGGIVAGSFFVIPSIFVLLLLSYL
                     AVAYSEVPAVTGLLYGVQPVVVAIVLEAVLRIGKRTLKHPALILFAVASFVGIYFLSI
                     PFPLVVGLAALAGYLLHFRYPEVFQAQGHGDAADGGEDLEQSAPPSMLRNLKLLALFA
                     ALWALPVGALYLWRGGGDVLVDEALFFTGAAFVTFGGAYSVLSYIADAAVNSHGWLTA
                     GQMVQGLGLAESTPGPLIMVTQYVGFLGAWKFHGPFDPLLYGTLGALTTTYVTFLPCF
                     LFIFLLAPYIELLSHNRRLGAALVGVTAAVVGVIVNLAVFFAGHVLFPNGLASLDLFA
                     LVVAVVSFAVLQRFKVPIYVLVPVGALAGMAWTLL"
     gene            complement(15154..15750)
                     /gene="cysC"
                     /locus_tag="VFJ72_06160"
     CDS             complement(15154..15750)
                     /gene="cysC"
                     /locus_tag="VFJ72_06160"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET7479082.1"
                     /translation="MENVSRRNSGERGFTLWFTGLSGAGKTTIAEIVEHELRERERRV
                     EVLDGDIVRTNLSKGLGFSREDRNINVLRIGFVANLLTRNGVGVIVSAISPFKEARDQ
                     VRRRIVDFVEVFVDAPLEVCAERDVKGLYKKAFSGEIPQFTGVSDPYEPPNAPDLVLK
                     TAEEPAEESARKVIEKLEFFGYLRSPSLDDAEYNWNSR"
     gene            complement(15797..16972)
                     /gene="sat"
                     /locus_tag="VFJ72_06165"
     CDS             complement(15797..16972)
                     /gene="sat"
                     /locus_tag="VFJ72_06165"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET7479083.1"
                     /translation="MITTQHGTIAPHGERLIDRVVAQEERTERLREAESLPKVILGER
                     TLSDLQMISTGVFSPLEGFMVREDYEGVVDEMRLSNGLVWSIPITLAVDEGTARSIRG
                     GSEVALVNGAGEPVATMTVEDIYTYDKEREARKVYRTTDERHPGVAHLYRQGDVLLGG
                     KVDLLQQPNRVRFPGYYYDPAQLRAVFAEKGWKRIVGFQTRNPVHRAHEYIQKSALET
                     VDGLLLNPLVGETKPGDIPADVRMRSYETILDRYYPEDRAMLAVFPAAMRYAGPREAV
                     FHAICRKNYGCTHFIVGRDHAGVGDYYGTYDAQHIFDEFEAEELGITPLFFEHTFYCR
                     ECGAMASAKTCPHDQDAHVFLSGTKVREMLANGEYPPPEFSRPEVVEVLIEGLRESS"
     gene            complement(16969..17733)
                     /locus_tag="VFJ72_06170"
     CDS             complement(16969..17733)
                     /locus_tag="VFJ72_06170"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479084.1"
                     /translation="MNEKQPVALWAVPRSISTAFERVFVERDDFEVLHEPFSASYYRS
                     EDRLSSRFADEETKPENNYENVLQDVLRPREQRVFLKDMAYHAKPLIGPDLTSRFVNT
                     FIIRDPKYVLTSMYKMWPDFTFEEAGYEELYRLFRICEEAGQEPVVVDAMTFSENPVE
                     VLSVYCERVGIPFREDDLTWQPREVRRWENWEGWHDVAQRSSGIKRAERRDPVLPREL
                     EEMYERCLPAYYDLAAHAIHGTRGSKRATTMREEGR"
     gene            complement(17871..19304)
                     /locus_tag="VFJ72_06175"
     CDS             complement(17871..19304)
                     /locus_tag="VFJ72_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7479085.1"
                     /translation="MTDRPNVVVIVADTFRRDHLGAYGNPFIRTPYLDEFARQSVVFD
                     EHTISSFPTMPARADILTGTFSYSHMGWEPLPRHLATLPEVLSKAGYTTMGVVDTPFF
                     IRNGFGYDRGFDDFVWVRGQGDDTRPHERADARSTWRSEEDRMVARTMTEAERWLERH
                     YDEQFFLYVDTWDPHEPWDAPEYYTAQYREGYSGEQIYPAYGNWKDAGLKRDDVDLGH
                     ATYCGEVTMVDFWIGRLLGKLDALGLRENTLVFFTSDHGFYFGEHDYFGKAEWVHDPE
                     ASISQDSSLPEWLADSWLLTVERSPLYRELTNVPMMVRGPGLEPGRRQALTTAPDIAP
                     TILELTGLGDAPATMVGESFGDVVAGRRDEHRPFVVSSWPLYLAEGEITRAVDSKARR
                     IANYMPITVTTRERSLILGGPDDEPEIYDLVNDPGEQNNAWDELGAEGETLAEHTLSF
                     LERIGTPETYLTPRRKALEGWRRVVRS"
     gene            19640..19831
                     /locus_tag="VFJ72_06180"
     CDS             19640..19831
                     /locus_tag="VFJ72_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479086.1"
                     /translation="MKRELDPWYELWERVGSEGLDNWYEVRGAAGEAGNTRGCRSGVG
                     DPDGELEGLPDTIELGLDG"
     gene            20054..20338
                     /locus_tag="VFJ72_06185"
     CDS             20054..20338
                     /locus_tag="VFJ72_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479087.1"
                     /translation="MAKKATGGGVWNGDWRFAMLKSVGVGALLFSAVVSAIVGAGIVF
                     LATVLAAWILFPVLVGPRNVLDAGCLFTMISLGIVVVVSLAQYGAGSLLP"
     gene            complement(20342..21655)
                     /locus_tag="VFJ72_06190"
     CDS             complement(20342..21655)
                     /locus_tag="VFJ72_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7479088.1"
                     /translation="MPDYPNILYVHSHDTGRWVEPYGYPVRTPNIQQLAEEGVLFRKA
                     FCAAPTCSGSRACFLTGQYAHSNGMVGLAHRGFSLKDYSHHMVHTLREHGYWSALVGE
                     QHISKKPDVIGYDEVVKIDTNHVDDVTPVTLDILTRNVRQPFFLSVGFFETHREFFQP
                     SSPRKANYVLPPPNLPDTPEIRQDMAAYHASAHSLDRGVGAVLDALDAVGLAEDTLVI
                     CTTDHGLAFPGSKATLYDRGLGVMLILRGPGGFTGGRASDALVSHIDVFPTICDVAGI
                     EQPEWLQGESLVPLANREADEVRDAIFAEKTYHVAYEPERCVRTRRWKYIRRFDDHPT
                     PVLANIDDGPSKDLLLASGWAERPVPEEQLYDLLFDPNETRNLAYEPEAAGVIDELRG
                     RLKSWMVETEDPILDGPVAAPEGAEINGQDQRSPNDPPTVVRAEG"
     gene            complement(21656..22540)
                     /locus_tag="VFJ72_06195"
     CDS             complement(21656..22540)
                     /locus_tag="VFJ72_06195"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HET7479089.1"
                     /translation="MTVAERSYLICATPRSGSTMLCAALDGTGIAGHPAEHFEVLLET
                     GLPRQPRDYFQRSNDPDVWALLDDAEFRDVLGEYGGRYAEHPARRDPSWRPPDFEALL
                     SAALEKGTTENGVFGTKIMWAYFRDFVRLARRTPGLNDVPPCAVPAAVFPELGRFVWI
                     RRGDTTRQAVSLWKALQTQQWRRDTDEESERRDLRFSFAAIDHLRLRIDEHNEAWTAF
                     FDQCGGEPVEVVYEDLVDDYEDNMMRVLRNLDIPIPEGFTLEKPRMKRQADALSEEWV
                     QRYNEQSNVKVGSYSGKD"
     gene            complement(22537..23031)
                     /locus_tag="VFJ72_06200"
     CDS             complement(22537..23031)
                     /locus_tag="VFJ72_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7479090.1"
                     /translation="MSATDDLLRNNEAYAQSFDKGDLPIPPAKGVVVVACMDARLNVF
                     GMLGLEEGDAHVIRNAGGVITDDEIRSILISQRLLGTTEIILIHHTDCGMLTFTDDEV
                     KAQIQEDVGIKPGFALEAFPDLEGDVRQSIARIKASPFIPHKDDIRGFVYEVETGRLR
                     EVEA"
     gene            complement(23050..>23877)
                     /locus_tag="VFJ72_06205"
     CDS             complement(23050..>23877)
                     /locus_tag="VFJ72_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HET7479091.1"
                     /translation="YEDILKLPSVTVARMLECAGNGRIFFEERQGREAPGTPWRLGAV
                     GVAEWTGVPLREVLLRAGLKKSAREVMPESLDAVRMRRPLPIEKALEEDVILAYAMNG
                     EPLPADHGFPVRVIVPRWAAVASVKWVGRIHVAEERLFSPWNTDKYVMTGGSFGTVRE
                     PVTAQVVKSALELPWPASLPEKRCRISGRSWSMHGAISRVQYSIDGGPWREARLIGPN
                     IPGAWARWYFYWNAKPGRHEIRARATDEAGYTQPEETTWNDLGYLYGGIVGHPINVY"
BASE COUNT         4010 a         7645 c         7719 g         4503 t
ORIGIN      
        1 cggaagccta cggcccgttc acgaacgaga aggtcgtggg cgaggcgctc gctccgttcc
       61 gggacgaggt cgtgatcgcg actaagttcg gcttcaagga cggcgacccg caggcgggcc
      121 tcgacagccg gcccgagcga atccgccagg tcgccgagga gtcgctcagg cgcctgaaga
      181 ccgaccgcat cgacctcttc taccagcacc gcgtcgaccc ggacgtaccg atcgaggacg
      241 tcgccggcac ggtgaaggac ctgatccagg agggcaaggt cgagcacttc ggcctctccg
      301 aagcgggggt gcaaacggtc cgtcgcgccc acgcggtcca gcccgtgacg gcactccaga
      361 gcgaatactc gctgtggtgg agagagcctg aagaagaagt gctgccgacg ctcgaagaac
      421 tcgggatcgg cttcgttcca ttcagtcctc tgggcaaggg cttcctcacg gggaagatag
      481 acgagaacac cacgttcgac agttccgact tccgcaacat cgttcctcgc tttacggcgg
      541 aggccaggag ggctaaccaa gccctggtcg atctgctcgg cgagatagcg caacggaaga
      601 acgcgacacc agcccagata gcgctcgcct ggctgcttgc ccagaagctg tggattgttc
      661 caataccggg tacgcggaag ctggagcgct tggaggagaa cataggagca gtcacagtcg
      721 aacttacttc gagcgacctc cgtgagatcg agagcgcagc ctcaaagatc accgtgcaag
      781 gggcccggta ccccgaagag ctggagcgaa ggatcgatcg ctgagaggcg caggatcgtc
      841 cgtccgggtg gtgcggtagg ctgcggcggg ccgagcctcc gagccatacg ttcacctaaa
      901 gttcggaaga acccggaatt actgagtagg tctggtaggg gcgcgtagcg caggaagcta
      961 ccgaaacgac catacgtaaa acatatctaa acgtcactaa actagtattg gacatatgga
     1021 atgctgttgg caataatggt cgacgtagct taaagggaaa ggagagaaac aagtggagaa
     1081 cattgaggca aggcttggtg gggaggacat tcacccgccg acatcgatca ggaaggtggc
     1141 tctcgccagc ttcatcgggt ctaccgtcga gtggtatgac ttcttcgtct acgggacggc
     1201 ggcggcgctg gtcttcgggc agttgttctt tccggagtcc actcctctgg tcggcaccct
     1261 gtcggccttt gccacgttcg cgataggttt cctgttccgg ccgctgggcg gagcgttctt
     1321 cggccatttc ggagaccggg tggggcgcaa gtcgatgctg gtcatcacgc tcttggtgat
     1381 gggtacttcg accgtcctga tcgggtgcct cccgacctac aactcgatag gcatactggc
     1441 gcctatattg ctcgtggcat tgcgagctat acagggcttc tcggtcgggg gcgaatgggc
     1501 cggcggatcg ctgatggtcg tcgagcatgc gccgccggga cggaggggat tctacggtag
     1561 ctggccgcag gttgggccgt cggcggggac gctgctatcg gcgggagtgt tcgccatctt
     1621 ctcggctctg ccggaggatc agttcctggc ctggggctgg cggatgccgt tcctcctgag
     1681 cgcggtcgtc gtgatcgtcg ggctcgtgat ccggctcagg ctcgcggagt cgccggtctt
     1741 cgagcaggtc aaggagaccc acaccggggc ccggctgccg gttctggacg tcttccgcac
     1801 caacgccaag aacatcttcc tcatcgcggg catgcgcctg gcgataaaca ccaccttcta
     1861 cgcctcgact gtcttcgccc tctcctacgc gacggagcag ttgggcatcc ccaggtccac
     1921 gatgttgacg ggaatcctca ttacggcggc tctcgggttc gtgagcaagc cgatctacgg
     1981 cgcgctatcg gacaggatcg ggcgccgacc aatctacgcg gcgggttcga tcatcggcgc
     2041 cctgatggcc ttcccgttct tcttcgccgt ggagtcgaag tccgcgatcc tgatcttcct
     2101 ggccttcttc ctcattatca atatctccca cgacctgaac gacgccgtcg agtcgtcatt
     2161 cttctcggag ttgttcggca cgcgggtccg ctacagcggc gcggcgttgg gtcatcagct
     2221 cggcgcggcc gtcacggggt tcaccccgct catcgcggcg gcgctggctg cggcgggcgg
     2281 ctggacttat gtcgccgtct acgtcatgtt cgcctgcctc gtgtcggccg gctgcgtctt
     2341 catggccgcc gagacgttcc gtaaggacat cgccgaggtg gaccctctgg agcgtcagat
     2401 catgacggag ccacgagtcc gataaaagag gagagagcgg tccggggcca gtcctcggac
     2461 cgcttccctc ttccgcgacg agcgtatgat ggttcgagaa aagagatcca actccgcgga
     2521 ggtgtctgtt ggctagggtg ttcgttctca acggcgtgaa cctgggtgcc ctgggcacgc
     2581 gccggccgga ggtctacgga actctgaccc tcggcgacat agagcagagg ctgcgcgaga
     2641 gcttcccgga ggtcgacttc gagtttcggc agacggacta cgagggtgag atggtcggct
     2701 acgtgcggga agcgagaggg agcgatggga tcgtgatcaa tcccggcgcc tggacccact
     2761 acgcttacgc tctgcacgat gccctggagg cggtcgaagg tgtcatcaag gtggagactc
     2821 acctctcgaa cgttcacgcc agggaaccct ggcggcggca ctcggtcatc tcgcccgccg
     2881 tcaacgccgt ggtggccggg atgggtccct acggctacga ggccgctgtc ggttacattc
     2941 tcagacaact aaaggcgtga actgatgcaa aagaagtctc tgacattccg gctggatccc
     3001 cgcggaatcc cggaggtgtg tggatgaagg cgttcgtaaa gacgggtcat caaccaggag
     3061 aagccggcgt cagggacgtg tcggtcgcca gccccggcct cggcgaggta ctgctccggg
     3121 tagcctcctg cggcgtctgc ggcagcgaca tccacgcctt caggtcggac gccgggttcg
     3181 agtgggtccg ggcgccggta acgctgggcc acgagttctc cggtacggtc gaggaggtcg
     3241 gaccggacgt gactcgggtg agggaggggg atcaggtcgt cgcggtcgct atacagggtt
     3301 gcggacgctg cgaagcctgc ctcgccggtt ccacgcagtt gtgccaggat cgcgtcgccg
     3361 tcggactctc ccgcgacggc gggatggccg agtacgccgt gatgcccgag cagcacctcg
     3421 tgcctgttcc cgaggggtta gatctcaccg tggcggccct gggcgagccg ctctccgtgg
     3481 ccgtgcacgc cgtaaacgtc cgggcggaca taaagccggg acagaacgta gtggtgtcgg
     3541 ggcccggacc cataggcatt ctctgcggga tgctggcccg gctccgagga gcgcaggtgc
     3601 tccttaccgg ggtaggaaac gactccgagt ctcggctccc cgcggcggag cgcgtgggac
     3661 ttcggaccgc gaacctgagc gataagcccc tggaggagca cctgcgggac agcttcgggg
     3721 agcctgctcc cgacgtctgg atcgagagtt ccgggtccgt gagggcgctc gactccgccc
     3781 tggagtccgt ccggccgggc gggacggtcg tcgtggtcgg tatctacgcc gaggagatgc
     3841 gcttttcacc taccgcttca gtgcggaggg agttgagcct gttgttcagc tattcctgca
     3901 actacgcgga ttacgaggag gccctggacc tgctgggcag cggcgccata gaccccggtc
     3961 ctctcctctc gaaatatccg cttcaggatg cctccgaggc tttcgaggcg gtcggcaaag
     4021 gccgggcagt aaaagcgatc ctcgtccctt gagccatgag atcaggagag aaaaaatgaa
     4081 cacagtccca gaggtaggca tagaccacct cacgatgctg gacgtcccgc cgccggatct
     4141 ggtgaccatc gcccacgagg cgggcttcga ctccatcagc ccccgcgtgg ccgcctcgac
     4201 tcccagcgaa gatccctggc ccatgactcc cggctctctt atgctcgaag agaccgcgcg
     4261 tcgcctggac gaaaccggca cgcggatact cgccgtggag gtcgtccgca tcggtcccga
     4321 tacgaagcgg gaggactacg agggcgcgct ggaagcgggc gcccggctgg gagcgcgcta
     4381 cgtgacggtc aacagcgacg atcccgatct cgaccgcgcc tccgagacgt tcgcggctct
     4441 gaccggcgcc gctagtccct acggcctgcg ccccatcatc gagccaatca tctacaccca
     4501 ggtatcgaac ctggacgagg ctatctacat cgccgagcgc tccggcggag gcggggtact
     4561 gctggacacc ctgcacttcc agcgctacgg gggagacctc ggacagctac gctccctcga
     4621 cccggacctg ctctcctacg tgcagctctg cgacgcgccg ctggcgccgc caaccggact
     4681 tccgcgtcct ctcaaactgc ctcgcggaca gtccaccgac ggcaccgacc tgcagataga
     4741 gagccgggcc atgcgaatgc tgccgggaga aggagagctg ccgctggcgg agttcctcgc
     4801 cgcgctgccg ggagaaatcc ccgtcagcgt ggaggctcct gtaatgtcgc tgcgggagac
     4861 cctgaccccg atcgagttcg cccggcgcgc tcgtgaggcg gtggcgaacg tactgtcggc
     4921 aacgcgcgca agttgaagaa gccgcgatga acgttgacca tccattgcta ccagagaggg
     4981 tcgacgcgct cgtcgtgggc ggcggcatgg ccgggctggc agccgcgatg gtcgccgcgg
     5041 agaacggtgc gcgggtcctg gtggtcgaga aaggtccgcg acccggcgga tcggcggcgc
     5101 tctcggtggg gatgttctgg actgtcccgg acttcgatac cctgcgccgg cgcgtgccgc
     5161 ttggcgaccc ggacctgggc cgggctgtcg tggaagacta cccggcggcc atcgagacga
     5221 tacgggagat gggcgtctcg gtaggggagc gggtctcggg cgtcatgacc gtcgggatcg
     5281 ggcactccat agacgttcac gcgctgcttg aacacgaggt tgccgagatc gaacgttccg
     5341 gcggacgggt cgcgtgcgac accaccgtgc ggcatcttct caccgacgac gaaggcgccg
     5401 ttaccggagc ggtagtacgc gggccgggtg gacagagcgc catcctcgcg gacgcgacga
     5461 tcctggctac cggcggcttt cagggggatc cggggctact cagcacgtac atggggccga
     5521 actcggaccg tatgctggta cgctcgaaca ggggtagcgt cggtgacggc ttgcggctcg
     5581 ggcgcgatgc tggtggagct acgagccggg cgatgagcag cttctacggc catctggtgc
     5641 cgagcccgct gtcgagtttt accgaggacc agtacatgcc gctggcgcag taccactcca
     5701 accactgcat cctcgtgaac cggctcgggc gccggttctg cgacgagtcg cagggcgacg
     5761 aggtctccgt ccaggccttg ctcgcgcagc ccgaggcccg cggcgttctg ctgtgcgacg
     5821 agagagttcg gagagagtac gtggttagcg agcccttccc gaacgccggg ctcctcgacc
     5881 gtttcgaaag agccgcggag gccggaggcc gcctgacgag ttctgacacc ctggacgggc
     5941 tggtgggcca ggtcgccggg tggggcatag acgccgttca actcagagcg acgctcgaat
     6001 cctatgcggc ggccgcgcgg cgtgagcccg taagcctgga cgcgccgctt cctaccgggc
     6061 ccaggccgct cgtcgagccg cctttttacg cgctggaggt gcagcccgcg atcacgttca
     6121 cctatggcgg cctgcgaacc gactcggacg gccgcgtcct cgaccatgac ggcgtgccgg
     6181 tcggtggact ctgggccgcc ggagtggatg ccggtgggtt gtcgaactgg acatacgcgg
     6241 gcggtctggc ccccgccttc atcaccggcc ggcgggctgc gtcctccgcg ctcgccgctc
     6301 tggacccgga agcgcggcag gacccggcgc gccctctata atggtcggcg tgttggattc
     6361 gatgaacaac ccggcgagac gtagatgatc agcgggaaga cgcggctcct ggctctgatc
     6421 ggtcatcccg tgagccacag cctgagcccc gcgatgcaca acgccgcctt cgccgccgat
     6481 ggactggact acgtctacgt ctgcctggat gtggatcccg ccgacctgcc cgcggcggtg
     6541 gagggcgcgg ccgccctgaa gctgcgcggc ttcaacatca ccatgccgca caagcgggcg
     6601 atgattccgc ttgtggacga gctggacgag ggagcccgcg tctccggagc ggtgaacacc
     6661 gtcgtgatcg aggactccac ccttcgcggc tataacaccg acggaggcgg gatggtcatg
     6721 gcctgccggg aggccggcat ccaactatcc ggccggagcg tcctgatcct gggcgccgga
     6781 ggcgccgccg ccgccatcgc cgtagcgttc ggggaggcgg ggatcggcga gctgcacatc
     6841 gccaaccgca gcgtggagca cgccgcggag ctgcgggaga agctgcgcgg ggcgggcatg
     6901 gagaaggtga cgatctattc tctcgatgcg ttggatcagg cgttctctga agcggagatc
     6961 gtcgtcaaca cgacgcccct tgggatgaag gaggcggatc cactacccat cccacccgag
     7021 tatgtgctta aggaccgcgc gttctgcgac gcggtctacc gtcccggagc ggagacgcct
     7081 ctcgtccgtc tggcgcgcga gcgaggcgcc accgtcgtcg ctggcggccg gatgcttcta
     7141 taccagggtg tgctggctca gaagctctgg accggacacg aaccgaacgt caaggtcatg
     7201 gacgccgctc tctcctaaag gcgcggcccg gctacctcgg tacggtagag ccctgtagcc
     7261 tccgctcgct caccacgagg accggacggc cggcccggcg gaggacggtc tccgagaagc
     7321 ccgggccaca gaagatccgc tcgaaccacg gacgtctgtg gcctccggtt attaccaatc
     7381 cggcgtcgat ctcctcggcg aggcggatta cctctttctg cggccagcct tccctgtaat
     7441 aggacgccgt aacgctacac ccgagctcct ccaaccgcct tacgcgatca tcgagaagcc
     7501 tgataccgtc cagcttcttc cactccagca gagcctcact ccgctcacga agcgcggtgt
     7561 tgggataggg tagctcgggt gttaaatgaa ctacgtgtac gacgtgcaac tcggaacctg
     7621 tgccgtctga taactcgacc gccgtctcct cggccagggc cgcctcccaa gttccgtccg
     7681 tcgcgagcag tatcctcgtc gggaacatac cgcctcttct cattagatgc tacttacaca
     7741 taaatgctat acagcggata ccgtcttgca agcgcggttt gtgaatcgga ccacccggta
     7801 ccgtggcaga ggctggcgaa cgtcgtggat tagtcaggcg gaagaggcgc ggtcgaaggt
     7861 tttctggaag gctctagggt gattcttctc cctgaacact cgtgccggag ttgctctgca
     7921 cgagggccaa aatgcgccgt ctcaggccgg cttcgagggc gtcttccagg ttcgcggtcg
     7981 agccgcagat caggacgcca gcggggatcg ctatgaccat caccggggct tgcgtctctt
     8041 cataactggc gaaggccgcc tctccacggc cctgggccgt gagcacgttc cccagagagt
     8101 cggcggagaa cgcggtccac tcgaacggga tcaccctggc gaacgtaatg tactctgcga
     8161 acgcgaaggc ggctggcagc tccgtgctgg cgtgctggac ttcctgggca aacccctcgt
     8221 ggaacctctg caagaactca tccacgccgc cggataccag gcacgccgcc aggctgcgca
     8281 ccacgccgcc ggcgggaacg tccgccagag cctgcagtcc aagggcctcc agcggtccgt
     8341 tcggcgcccg gaacaggtag gagtcccctc caagagaccg cgacggcacc actacgaagt
     8401 gcgccgagag gccggactgc ccgtccatga cagtcgtctc gtagatctgc gagacggata
     8461 gcctcgctcc gaatccgtgc cactcatcca tgttcctata gtacctcacc gttttgacga
     8521 gttccgcgcc gcgttgatcg gttgagcatc aaccgtccga ggcgtctccg gcgccctgga
     8581 gtgaatgctg ctcgaagaac cgcatcatct ccgcggaggc gtcggggcct gtggggtcgg
     8641 tgtacgagcc tcgcgagtct ccacccgacc aggcgtgtcc gagcccgtgg acggtccact
     8701 gctccacgac cgtgcgccct tcggcatcct cgtatgagac gcgcgtgtag gagcgcccgc
     8761 cgggcgactg tccctggctc tgtgcagctt gcgcgcccgg cgtccctttg agtccgtggg
     8821 agatcagatg ctccgcgttg cgcgggtgga cggtagtgtc gcggtccccg tggaacacta
     8881 tcgtcggcgc catcgcgcct gctttaccgg cgtgcatccc gtttcgcatg gcggtgaacg
     8941 cggaggacat gtcgtgagcg gctccaggag cgagaccgga gtggaccccg actgcggcgt
     9001 agacgtccgg gtaggtcgcg cccatgaccg ccgccatcgc cccgccggcc gacatcccgg
     9061 ccacgtatac gcggtccgcc gcgacgtgat actcgccgac gacttcgcac gtgatacccg
     9121 cgatgagtga aggctcgccc tggccgcgct gctggtcggc ggcctggaac cagttccagc
     9181 atttttgcat gttggcgttg cccgactgag ccggatacgc gacgaggaac gtgtgctcct
     9241 ccgcgagcgt gttcatgccg gtaccggatg cgaaatcgtt ggggttctgc gtgcagccgt
     9301 gaagcatgac gaccagcgga accgcctgtc caacgtagcc agttgggatg tacagcttgt
     9361 aggcgcgggt gccgaagcga ttagtgtagg accgctcaat gaaccgtccg ggctccgtcg
     9421 gtgttccaag tggttcaacg gtcagggtcc ctggagagcg cggcatgccg cgggaagtcc
     9481 ggtgcggtct ggcgtgcctg ggagcgggac gcagagtttg cgtgtcctga tcttgttgcg
     9541 atgctgtcgc ggatcgggga tcttcgttta tggcgtgcag gatagcctgc agaggatcgc
     9601 cgaagttgct cgaagtatcc gtcgctccgg tagaaccgcc ggtaaagccg cctccgagag
     9661 cgcgttgtat tagggccgtc gcttcggcga gtttcccttc tcgtgtgagc cgcgtggcct
     9721 ccgccatgcc gtcttgtatt gggttactca tctttctcct ctcttacgtt atgacgggcg
     9781 gatccggtcc gccagcgccg ctttcactgc agggctcgcc cggaaggagc ccagcaccac
     9841 aatcgattcg atggtctcta gagccagttc gggggagacg tcatcagcga tgcttgcgag
     9901 tccgaggacc ttgatctcca gcatctcgcc ggcgtcgcgc accgcttcca ggtcgcggcg
     9961 cgtgtagtgt tgcagaccca gaaccagcgt cttgcgagcc accgtctgcc ggaccgtttc
    10021 agcgtgcgtg gagatctggt tccggatcgc cgttcggatg aagtccgtgc ggttcgcgta
    10081 gaaaccctcc tgcaccagca ggtcgatctg cccgagatct accatcccca gattgatcgt
    10141 gatcttctcc aactcactcg tagacatctc agtagcatct cctgaacatc tctataccat
    10201 ccatacggat gttaacacaa gtgctgggca tgttgtcaag agtgatgtat cgttcgggag
    10261 gcaggtgagt ttgtagagag gccggaccgg ggcagggtga gatgccatcc gccgctgcga
    10321 cggcggtaat cgctgaggtt ggaccccgat aatcaggagg tattgagtga gcttgcgatg
    10381 ttgtgtaggg acccgcttcc ctggtgggct ggcggcgtcc tacttgactt gtgtcaggta
    10441 tggcgcgtta agcgccgggg cggggtggag tgccgggagg cagaggtgaa gatccgtcag
    10501 gccaacggaa tcggcagggt acatgtagcc aagccccaga tgaaggagac gtactgcggc
    10561 agaccggtag acgaggagga gtgggtgacc acctccaggg aggctaactg cgtcgcctgt
    10621 gcccgtgcgg gggcgcccag gccggaggga tcggggcgcg gcgagtagcg aggtcactag
    10681 ctccgccgga gcctgctgaa cctctcgtcc gccgggcgca acagctcaaa ccaggcacgg
    10741 cttttggcct gagagcgagc atccgccttt attccttaac caggctcagg gagtctggtt
    10801 tatgggcggc ttccctgccg ctcttctggc tctcgaccag gtatcgaggg tcgtcctcgg
    10861 aggcggatat cttcgttcca ccgacctgag tatcagccgt gagcttcttc tttaccttgc
    10921 cccgcgtctt gccctgcggg gtgttccact ccaccttgtc gccccgcttg aactcgtgcg
    10981 ccatcgcgct ccttccgttt ggttcacggt acttattaca ggctttcgtg ctccgtgaat
    11041 gcgaacgctc ttactggaag gagcgcggac agctggagac cggctcgtaa gccggtcccc
    11101 ggtccttgct tcgagtgctt ctaggagatc gtccggtaag cgaggaagcc gccggcggcg
    11161 aggagtagag cggctcctgc aatcacggcg accgaggctc ctccggtatc cgggagcacg
    11221 ctgacggcgg cgttggaatc cacgttctcc gaagagggtc caccatcgct gctagagtca
    11281 tcctcgtact ggccgctggc cgctccgtcg tcgttattgt ccgcggaggc gttgccgtcg
    11341 gggtgagttc cggcgggatc gccgccgatg gtatctcccg agaaggtgta tgaagcgtcc
    11401 acgataaatc ctgtatcggc gaccttcgag ccttcggcca ctacctcctg ggagagttct
    11461 gagccctggg agcctatgat gcggtagtcg agggtgctgc ccgccggagc ggagaatgcc
    11521 atctcgttgg tagagccgcc gtcccggcac tcggggatgc ccgtctcgac catcgccgcg
    11581 tccgtcgtgc agataacgcc gccagctcca gaggaggtct cgacccagaa agatacgttc
    11641 tccggcgccc agccgtcgag cgtcaaccgg aacttgccgt cggccgtgcc gcctgagagc
    11701 gcctcggcgt cctgggccat gacgggcacc ggggccgcga ggagcgccat caacatcgcc
    11761 gccagaaccg ccgatctctt cagcatgatc tcctcctccc gtagtttacg tctgcattgt
    11821 aagtgcggtg ggtaacaaat agataacaag atgtgtattg tttggtaaca gttttgcggc
    11881 gggaagcggc gctctgtcgt agaatccggt agagatagaa tggtattggg ccggagccgt
    11941 agcgttccgg cccggtttag ataggaggcg cggtgcagac ggaggtcatc caatttattg
    12001 gagctttgat cttgatggcg ggcatattgg tagggtccta ctggttcgtg tacgcgccca
    12061 taaagaacag cgaccgtcgc agggagcagc agagatcaga agatcagcga tgactctctc
    12121 gccctgccgg aggatctagg attgcaggtt tcggcgaaga ccgattatgc gttgcgggcc
    12181 gctgcggagc ttgcgcgggc ggcggccgac gaggcgggac cgatgaaggg cgagttcata
    12241 tccgtctccc agggcatccc gaagaagttc atggagaaca tcctgcacga cttgaagaga
    12301 gccggcatcg tgcgtaccca gcggggcgcc tctggcggat actggctcgc cctgcccgcc
    12361 gacaaggtct ccgtggcaga cgttatccgg gccgtcgagg gtcctctggc gaacgtccgg
    12421 ggcgagtggc cggaggccgt cgagtacacg ggagccgccg agcacctcca ggaggtctgg
    12481 atcgctgtcc gcgccaacct gcgcgccgtc ctcgaaaccg ttaccctcgc cgacatcgtc
    12541 gaaggcaagc tcccaactcc agttacgaaa ctcacccgcg acccggaggc ctgggtccac
    12601 cactaggccg gctcagtaga ccgttaccgg gtgggcgacc accgcgttgt agagatatcc
    12661 ctgttcgttg tgaggcgcgg cctccggttg cgtgtttccc gctatatccg tcgcgcggat
    12721 tcggatctcg tattctcccg gctctggctc ccactcgaaa cggaaacagg cccaggcgcg
    12781 gggcaaattc tgacccgtga gccgcgccgg attccacgat gcgccgccat ccaggctgaa
    12841 gtcgacgcgc gagatctcac ccgcccccga ccaggcgcgg ccctggatcc cacgccgccc
    12901 cgtcgcgagc cgcgccggcc agggaagctc cagcgcgctc ttgaccccct gcgccgttac
    12961 ggctgggccg agtcccggat tccgcgcctc gtagccggga ccggccagga cgtactcctc
    13021 cgtgttccac ggcacgtgga cgggctcctc ggagacgtat atccgcccga cccacttgat
    13081 gctcgctacc gcgtaccatc cggggacgac gacgcgcgac gggaacccgt ggtccgctga
    13141 gagaggctca ccgttcatcg cgtaggcgac tatcgtgtcc atctcaagcg ccttcccgac
    13201 cggcatcgga cgccgtcccc gctcctcgtc cagaccctcc ggcatgacct cgaccgcaga
    13261 cggcttgaga cccgccagcg agagcaactc tcccagcggc acgcccgtcc actccgcgac
    13321 gcctatggcg ccaagcctcc acagagcgcc ctcagcgccc cgtccctgca cctcctcaaa
    13381 gaacacgcgc ccgttacccg cgcactccag gttgcacacg accgacgcgc ttggcagagt
    13441 cagcaactct tcgtacccga tcttcagggc gcgctccacc cccgggcctt cgactcgcag
    13501 ggtccaggta gctgcgtcta tggtgggggt ggggccgtgg ttacggacgt agaagcggtc
    13561 tatctccgtc aggtaacccc gcccggacat cgcctcccag cgcatctccg cgttcttgcc
    13621 gaggtccacg aagagctccg gcggcaacgg cttctttatc ggacggtccc gttcacctgc
    13681 cacaaccggc aaggatacac tgcccggagc ttgataggcg atgaagcggc ggttattgta
    13741 tgctgaatta gtgaacaaga gggaggtgct ttggcgcgga aagaggtagg gtttggggag
    13801 gcgttgcgtt tctgggtgaa gctcggcttc atcaacttcg gtggtccggc gggtcagatc
    13861 tcgatcatgc accgcgagct ggtggagcgg aaacgctgga tctccgagtc ccagttcctc
    13921 cgttccctga acttctgtat gctcctcccc ggaccggaag ctcaacagct agccacttac
    13981 atcggctggc gcatgcacgg ggttctgggc ggtatcgtcg cgggctcgtt cttcgtaata
    14041 ccctccatct tcgtcctgct gctcctctcg tacctcgcag tcgcctactc ggaggtgccc
    14101 gccgttacgg ggctgctcta cggcgtgcag cccgtcgtcg tggcaatagt cctggaggct
    14161 gtgttgcgga tcgggaagcg gacgctcaag caccccgctc tcatactctt cgccgtagcc
    14221 tcattcgtgg ggatctactt cctctccatc ccgttcccgc tcgtcgtggg cctggcggcg
    14281 ctcgccggat acctgctcca cttccgctac ccggaggtgt tccaggcgca gggacacggc
    14341 gacgccgcgg acgggggaga ggacctggag cagagcgcgc cgccctcgat gctccgcaac
    14401 cttaagctcc tcgctctctt cgcggcgctg tgggcgttgc cggtaggcgc gctgtacctg
    14461 tggcgcggag gcggggacgt gctcgttgac gaggcgctct tcttcaccgg ggcggcgttc
    14521 gtgacgttcg ggggcgcgta ctccgtgttg agttacatcg cggacgcggc agtgaacagc
    14581 cacggctggc tcacggcggg ccagatggtt caggggctcg ggctcgcgga gtccacgccg
    14641 gggccgctga tcatggtgac gcagtacgtc gggttcctcg gcgcgtggaa gtttcatggg
    14701 ccgttcgacc cactgctcta cggcacttta ggcgccctca cgacgaccta cgtcacgttc
    14761 ctgccatgct tcctgttcat cttcctgctg gcgccgtaca tcgagcttct ctcgcacaac
    14821 cggcggctcg gggcggcgct cgtcggggtc acggcggcgg tcgtcggcgt gatcgtcaac
    14881 ctcgccgtct tcttcgcggg ccacgtcctc ttcccgaacg gactggcatc cctcgacctc
    14941 ttcgccctcg tggtcgcggt agtctcgttc gcggtgctgc agaggttcaa ggtcccgatc
    15001 tacgttctcg tgccggtcgg ggctctcgca ggtatggcgt ggaccttgct ataaagcaga
    15061 gcatccataa gccgccagct tcttgttctt gctgacggct tatggttttc cggcgactgg
    15121 ccgacgactg gcgcagtcgt tgtaaatagc gctctagcga gagttccagt tatactcggc
    15181 gtcgtcgagc gacggggatc tgaggtagcc gaagaactcc aacttctcta tgaccttgcg
    15241 tgcgctctct tcggccggct cctcggctgt cttcaggacg aggtccgggg cgttgggcgg
    15301 ttcgtagggg tcggagacgc cggtgaactg cgggatctca cccgagaacg cctttttgta
    15361 gaggcccttc acgtcccgct cggcgcacac ctcaagaggc gcgtccacga agacctcgac
    15421 gaagtctacg atcctgcgcc gaacctggtc gcgggcctcc ttgaacggcg agatggccga
    15481 cacgatcacg cccacgccgt tgcgcgtcag caggttcgcc acgaacccga tccgcagtac
    15541 gtttatgttc cggtcctcgc gcgagaagcc gagaccttta gagaggttgg tccgcacgat
    15601 gtccccatcg agcacctcga cccgccgctc ccgctcgcgc agctcgtgct cgacgatctc
    15661 ggcgatggtc gttttcccgg cgccggatag tccggtgaac cacagcgtga acccgcgctc
    15721 cccgctgttt cgtctgctca cgttctccat gcttactcct ttttgaaata ctccttacaa
    15781 tactctagca ctcctctcaa gaactctcgc gcagaccctc gatcagtacc tccacgacct
    15841 ccggcctcga aaactccggc ggcggatact cgccattcgc cagcatctct cgaaccttcg
    15901 tcccggagag gaagacgtgc gcgtcctgat catgcgggca ggtcttcgcg gaggccatcg
    15961 ccccgcactc gcggcagtag aacgtatgct cgaagaagag cggcgttatg ccgagctcct
    16021 cggcctcgaa ctcgtcgaag atgtgctgcg cgtcgtaggt gccgtaataa tctccgaccc
    16081 cggcgtgatc ccgtccgacg atgaagtgcg tgcagccgta gttcttgcgg cagatcgcat
    16141 ggaagaccgc ctccctcggt cccgcatacc tcatcgccgc cgggaacacc gccagcatcg
    16201 cacggtcctc gggatagtaa cggtccagga tcgtctcgta ggagcgcatc cgtacatcgg
    16261 ccgggatgtc gcccggcttt gtctcaccca cgagcgggtt caggagcagc ccgtcgaccg
    16321 tctcaagggc gctcttctgg atgtactcgt gcgcccggtg gaccgggttg cgcgtctgga
    16381 atcccacgat acgcttccag cccttctccg cgaacacggc gcggagttgt gcgggatcgt
    16441 aatagtagcc agggaatcgg acgcggttcg gttgctgtag tagatcgacc ttgccgccca
    16501 gcagtacgtc gccctggcgg tagagatgcg ccactccagg gtgccgctcg tcggtggtcc
    16561 ggtagacctt ccgcgcctcg cgttccttgt cgtaggtgta tatgtcctcg accgtcatcg
    16621 tggcgaccgg ctcgccagcg ccgtttacca gagcgacttc cgaaccgcca cgtatggagc
    16681 gcgccgtccc ctcgtccacg gcgagggtta tcgggatgct ccacaccaga ccgttcgaga
    16741 ggcgcatctc atccacgacg ccctcgtagt cctcgcggac cataaagcct tccagagggc
    16801 tgaagacgcc ggtcgagatc atctgcaagt cggagagcgt ccgctcccct agtatgactt
    16861 tcggtaggct ttcggcctcg cgcagccgct cggtcctctc ttcctgggct acgacgcggt
    16921 ctatcaggcg ctctccgtgc ggggcaatgg ttccgtgctg ggtggttatc atcggccttc
    16981 ctctctcatg gtagtagctc gtttcgaacc gcgggtcccg tgaatggcgt gggcggcgag
    17041 gtcgtagtag gcgggcagac acctctcata catctcttca agctccctgg gcagcacggg
    17101 gtcccgcctc tcggcacgct ttatgccgct actcctctgc gccacatcgt gccatccctc
    17161 ccagttctcc cagcgcctta cctccctggg ctgccaggtg agatcgtcct cccggaacgg
    17221 tatcccaacc cgctcgcagt acacggataa gacctctacc gggttctcgg agaacgtcat
    17281 tgcatcaacc acgacgggct cctgaccggc ctcctcgcag atcctgaaca gacgatacag
    17341 ctcctcgtag cccgcctcct cgaaggtgaa gtcgggccac atcttgtaca tggaggtaag
    17401 gacgtacttt gggtcgcgga taataaaggt atttacgaac ctggaggtga gatccggccc
    17461 gatcaggggc ttcgcgtgat aggccatgtc cttgaggaag acgcgctgct cgcgtggtct
    17521 caacacgtct tgtaggacgt tttcgtagtt gttctccggc ttcgtctcct catcggcgaa
    17581 tcgactggag aggcggtcct cggagcggta gtaagaggcg gagaagggtt cgtgcaggac
    17641 ctcgaagtcg tcgcgctcga cgaagacccg ctcgaaggcg gtagagatag agcgtggcac
    17701 cgcccagagg gcgacgggtt gcttctcgtt caagtgaacc tctctgtctc gggtgtttta
    17761 tgcggcgtcc ggcctcgccg gagagacgta acatgaggcg ggcgacaatg aaacgtcgcc
    17821 cgcctcgact gcccgcgtgg ttcccggatg cggcgggaag ggcccgagcc ttagctacgg
    17881 actaccctgc gccagccttc gagcgccttg cggcggggag tcagatacgt ctccggcgtg
    17941 ccgatgcgct ccaggaagga gagggtgtgc tcggcaagcg tctcgccctc ggcgcccagc
    18001 tcatcccagg cgttattctg ctcgccgggg tcgttgacga ggtcgtagat ctccggctcg
    18061 tcgtccggtc ctcccaggat gagggaccgc tcgcgggtcg tgacggtgat gggcatgtag
    18121 ttggcgatcc tgcgcgcctt cgagtccacg gcccgcgtga tctcgccctc cgccaggtac
    18181 agcggccacg agcttacgac gaacggccgg tgctcgtccc gcctccccgc aaccacatcc
    18241 ccgaacgact ctccaaccat ggtggccggc gcgtccccga gacccgtcaa ctccaggatg
    18301 gtcggcgcga tgtccggggc ggttgtgagc gcctgccggc ggccgggctc cagtccggga
    18361 ccgcggacca tcattggcac gttcgtcagc tcccggtaca gtggcgagcg ctcgacggtc
    18421 agcagccagg aatcggccag ccactccggc agcgacgagt cctgcgatat ggacgcctcc
    18481 gggtcgtgga cccactcggc cttgccgaag tagtcgtgtt cgccgaagta gaagccgtgg
    18541 tcggatgtga agaagaccag cgtgttctcc cgaaggccca gcgcgtccag cttgccgagg
    18601 aggcgaccga tccagaagtc aaccatcgtg acctcgccgc agtaggtggc gtgtccgagg
    18661 tccacgtcgt ctctcttgag accggcgtcc ttccagttgc cgtaggccgg gtagatctgc
    18721 tctcccgagt acccctcgcg gtactgcgcg gtgtagtact ccggcgcgtc ccagggctcg
    18781 tgcgggtccc atgtatccac gtacaggaag aactgctcgt cgtagtgccg ctccaaccac
    18841 cgctcggcct cggtcatcgt ccgcgcgacc atccggtcct cctcggagcg ccacgtcgag
    18901 cgcgcgtcgg cccgctcgtg cggccgggtg tcgtctccct gcccccgcac ccacacgaag
    18961 tcgtcgaagc cccggtcgta gccgaagccg ttgcggatga agaacggcgt atccacgacg
    19021 cccatagtcg tgtaacccgc cttcgagagt acctccggca gcgtcgccag atgcctcggg
    19081 agcggctccc agcccatgtg cgagtaggag aaagtccccg tgaggatgtc cgcgcgggcg
    19141 ggcatggtgg ggaaggacga gatcgtgtgc tcgtcaaaga ccaccgactg gcgcgcgaac
    19201 tcatccagat acggcgtgcg gataaacggg ttcccgtagg cccctagatg gtccctgcgg
    19261 aacgtgtccg ccacgatcac caccacgttt ggccgatcag tcattgctcc cctctccgat
    19321 ctatgtagct cgtcgttagc acctttacac actatttcta tattgtctac tacttgagtt
    19381 aacaacagcg gcatggtagc gaaacgagga tccaacgtca agcgcttata tgaacttttt
    19441 tgaaaatcgg agaaaatttg tgaacctgcc tctgtgcttc gctacgtccc tggcacgcgc
    19501 atggcgctgg aagccccgat ccctgaaaag cgaaagcccg gccgcgctct tgacaccggt
    19561 cacatggctc agtacagtga gtattgtaca taaaaactat aggcaatatt caaattaaag
    19621 taaggagggt gtgagagtca tgaagaggga gttggatccc tggtacgagc tctgggagcg
    19681 ggtgggtagc gaaggattgg ataactggta cgaagtgcgc ggcgcggcgg gggaagcggg
    19741 caataccaga ggttgccgtt ccggcgtggg cgatcctgat ggagagttgg aaggactgcc
    19801 ggataccatc gaactcggtc tcgacggtta gcgagcgctt catgaacaga gtattggcag
    19861 tcggggagca cgcaaggacg gagaacaata ctgtgcccag acaaatgcta acctacgagg
    19921 accactccga gcggcggaga gtagagcaat actcgtggct cggcgattac ttcacgcggc
    19981 tcgatggcaa agaagcccgt gaagagtact acgcgcggtt aggatcatac gcaagcccgt
    20041 ccgggaaatg agcgtggcga agaaggccac cggaggaggc gtctggaacg gagactggcg
    20101 tttcgcgatg ctcaagtccg tcggcgtcgg ggcgctgctg ttctcggcgg tagtctccgc
    20161 gatagtcggg gccgggatag tcttcctggc gacggtgctg gccgcctgga tcctcttccc
    20221 tgtgctcgtc ggccccagga acgtcctcga tgccgggtgc ctctttacca tgatctcgct
    20281 ggggatcgtg gtcgttgtca gcctggccca gtacggagcg ggctctctgc tgccttgagc
    20341 gttagccctc tgcccggacg acggtaggcg ggtcgttcgg ggaccgctgg tcctggccgt
    20401 tgatctccgc gccctccggc gccgctacgg ggccgtcgag gatcgggtcc tccgtctcca
    20461 ccatccagct ctttagccgc ccgcgcaact cgtctataac gccggccgcc tcgggttcgt
    20521 aggccaggtt gcgcgtctcg ttcgggtcga agagaagatc gtagagttgc tcctccggga
    20581 cgggtcgttc ggcccagccg ctcgccagga gcaggtcctt gctcgggccg tcgtcgatgt
    20641 tcgccaggac gggcgtcggg tggtcgtcga agcgccggat gtacttccag cggcgcgtcc
    20701 ttacgcaccg ctccggctcg taggcgacgt ggtaggtctt ctccgcgaag atggcgtccc
    20761 ggacctcgtc ggcctccctg ttcgctagcg ggacgagcga ctcgccctgc agccactccg
    20821 gctgctcgat gccggcgacg tcgcagatcg tggggaacac gtctatgtgc gagacgaggg
    20881 cgtcgctggc cctgccgccc gtaaagccgc cggggccgcg aaggatcagc atgacgccca
    20941 acccccggtc gtagagggtc gccttgctgc cggggaaggc gaggccgtgg tccgtggtgc
    21001 agatgacgag cgtgtcctcc gcaagcccca cggcgtccag agcgtccagg acggcgccga
    21061 cgccccggtc cagggagtgg gcgctcgcgt ggtaggcggc catgtcctgg cgtatctccg
    21121 gcgtgtccgg caggttcggc ggcggcaaga cgtagttggc cttcctgggc gagctcggct
    21181 ggaagaactc gcggtgcgtc tcgaagaacc caaccgagag gaagaacggc tgccggacgt
    21241 tcctggtgag tatgtcaagg gtcacgggcg ttacgtcgtc cacgtggttg gtgtcgatct
    21301 tgacgacctc atcgtagccg atcacgtcgg gcttcttgga gatgtgctgc tccccgacga
    21361 gcgccgacca gtagccgtgc tcgcgcagcg tgtggaccat gtggtggctg tagtccttga
    21421 gcgagaagcc ccggtgcgcg aggccgacca tcccgttcga gtgcgcgtac tgtccggtca
    21481 ggaagcaggc ccggcttcct gagcaggtcg gggcggcgca gaacgccttg cggaagagga
    21541 cgccctcctc cgcgagctgc tggatgttgg gcgtcctgac cgggtagccg tagggctcga
    21601 cccaccgccc cgtgtcgtgc gagtgtacgt acaggatatt cggatagtcc ggcatctagt
    21661 cctttccaga gtaactgccg accttgacgt tgctctgctc attgtagcgt tgcacccact
    21721 cttcggagag ggcgtccgcc tggcgcttca tgcggggctt ctccagggtg aagccttcgg
    21781 ggatcgggat atccagatta cgtagcaccc gcatcatgtt gtcctcgtag tcgtccacaa
    21841 ggtcctcgta gacgacctct acgggttcgc cgccgcactg gtcgaagaag gccgtccacg
    21901 cttcattgtg ttcgtctatc cgcagccgca ggtggtctat ggccgcgaag ctgaagcgga
    21961 ggtcccgccg ttcggattcc tcgtcggtgt cgcggcgcca ttgctgggtc tgaagggctt
    22021 tccagaggga gacggcctgg cgggtggtgt cgccgcgacg gatccagacg aacctcccca
    22081 actccgggaa gacggccgcc ggaaccgcgc agggcgggac gtcgttcaac cccggcgtcc
    22141 ggcgggccag acgcacgaag tcccggaagt aggcccacat gatcttggtc ccgaacaccc
    22201 cgttctccgt cgtccccttc tccagcgccg ccgacagcaa cgcctcgaag tccggcggac
    22261 gccacgacgg gtccctccgc gccgggtgct ccgcgtaccg gcccccgtac tcgcccagca
    22321 cgtcccggaa ctccgcgtcg tccaggagcg cccacacgtc cgggtcgttg gagcgctgga
    22381 agtagtcgcg cggctggcgc ggaagccccg tctccagaag gacctcgaag tgctccgccg
    22441 gatggcccgc tatccccgtg ccgtcgagcg ccgcgcacaa catcgtgctc ccgctgcgcg
    22501 gcgtcgcgca gatgagatag gaacgctccg cgaccgtcac gcctccacct cgcgcaggcg
    22561 tccggtctcg acctcgtaga cgaagcccct gatatcgtcc ttgtgaggga tgaacgggct
    22621 cgcctttatc cgggcaatgg actgcctcac gtccccctcc aggtccggga acgcctccag
    22681 cgcgaacccg ggcttgatgc caacgtcttc ctgtatctgg gccttcacct cgtcgtcggt
    22741 gaaggtgagc atcccgcagt cggtgtggtg gatgaggatg atctctgtgg tgccgagcag
    22801 acgctgggag atcaagatag accggatctc atcgtccgtt atgacacctc cggcgtttcg
    22861 gatgacgtgg gcgtcgccct cttcgagccc gagcatgcca aagacgttta gccgcgcgtc
    22921 catgcaggcg acgacaacga cgccttttgc cggcggtatc ggcaggtctc ccttgtcgaa
    22981 ggactgggcg taagcctcgt tgttcctgag tagatcgtcc gttgcgctca tcctttgtcc
    23041 ttcccagttt cagtacacgt ttatggggtg tccgacgata ccgccgtaga ggtagccgag
    23101 gtcgttccag gtcgtctcct cgggctgggt gtaaccagct tcgtccgtcg cccgcgcgcg
    23161 gatttcgtga cggccgggct tcgcgttcca gtagaagtac cagcgcgccc acgctccggg
    23221 gatgttcggg ccgatcagac gcgcctcccg ccaagggccg ccgtctatgc tgtactggac
    23281 ccgtgagatc gcaccgtgca tcgaccagga gcggccggag atacgacacc gcttctccgg
    23341 cagggaggcg ggccagggca actccagggc gctcttgacg acctgcgccg ttaccggctc
    23401 ccgaacagtc ccgaagctcc cacccgtcat gacgtacttg tccgtgttcc agggcgagaa
    23461 caaccgctcc tcggcgacgt ggatgcgccc cacccacttg acgctcgcca ccgccgccca
    23521 tcgggggact atgacgcgga ccgggaagcc gtgatcggcc ggaagcggct cgccgttcat
    23581 ggcgtaggcc aggatcacgt cctcctccag cgccttctcg atgggcagcg gccggcgcat
    23641 ccgcaccgcg tcgaggctct cgggcatcac ctcccgcgcc gacttcttga ggcccgcgcg
    23701 caggagcacc tcccgcagag ggaccccggt ccactccgcg acgcccaccg caccgagcct
    23761 ccacggcgtc cccggagcct cccggccctg ccgttcctcg aaaaagatcc tcccgttccc
    23821 ggcgcactcc agcatccgcg cgaccgttac acttggcagc ttcaggatgt cctcata
//
LOCUS       DASSMB010000095         9538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_20347, whole genome shotgun sequence.
ACCESSION   DASSMB010000095 DASSMB010000000
VERSION     DASSMB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9538
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20347"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFJ72_06210"
     CDS             <1..372
                     /locus_tag="VFJ72_06210"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HET7479092.1"
                     /translation="ARCPFGMPSVIENERHRNMPTSFWITCKTLDAAIARIESTGGVR
                     EAQEHLGEEKIEEIHTAHKTAYGTRVAGVREGGYVKCLHAFTALHLAGAIPNPVAEWT
                     LQRLQNSYPQDRCCTAGEPEN"
     gene            396..478
                     /locus_tag="VFJ72_06215"
     tRNA            396..478
                     /locus_tag="VFJ72_06215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:429..431,aa:Leu,seq:taa)
     gene            505..1614
                     /locus_tag="VFJ72_06220"
     CDS             505..1614
                     /locus_tag="VFJ72_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HET7479093.1"
                     /translation="MITAHADIVGSLLRPPELLEARELYAAGGMSAADFKAVEDRAVD
                     WALGLQERAGMPVVTDGEMRRESFQSQLVEAVEGFAGHTLDAWLWGEWHGDESVGDKT
                     TERPAALGVVGKLRRRRHLSVEEFVYARARTDRTIKVTLPSPSMYANLWSPELSRDAY
                     PTLDLFLEDVAEILREEVEELARLGAEYIQLDAPHYPLLLDPKTREFYERRGWDLDRW
                     LERGIELDNHVIGEHPGVTFAFHLCRGNQGSRWLVSGSYEPLARRVFGGIRAGRLMLE
                     YDDERSGGFEPLAHVPEDKVAVLGLVTTKTPRRETPEELETRIREAAEYIPLERLALS
                     PQCGFATSVIGNAVGVEDEERKLRVISETAGRVWG"
     gene            complement(1635..2765)
                     /locus_tag="VFJ72_06225"
     CDS             complement(1635..2765)
                     /locus_tag="VFJ72_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7479094.1"
                     /translation="MRRARWLFIAVLSLMFVVGPAGIAQAQSYEPQELRFLELINQYR
                     QQNGLEPLLLSAPLSVASEHHSEDMGTYGFFSHTTEGSSYYPIGSGHPERIAQEGYDY
                     NTYTAENLAYGQTTADEVFEAWRISPGHNTNMLGPYKVIGIGLVWVNGTPYWTTDFGA
                     YVDPSASNGGAGGASGGGESSNGAADATSPAKTPAPEAPDVTTQAAPETTSDTSPDST
                     ASGRKETAAADQYTVKQSADPVAKSQPSAEPSAETAASDQYADTEAPATTESAATKQA
                     TAPRTAARADSQAVESAPAEAGDSGQGGNQANAAETEPVAARETGEKSQSDAGQAPAA
                     TPAVASAEISILPDTGGVPLLPLAAGLALLISGSLMARRALR"
     gene            2904..4532
                     /locus_tag="VFJ72_06230"
     CDS             2904..4532
                     /locus_tag="VFJ72_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET7479095.1"
                     /translation="MKDSRKESDSSLRSVAFVALFGLAAAVFVFVVYVRTLAPTVLYY
                     DRPILLDSVMLQAQATVLGITGPTGEPTWVMLTHLFTKLPFGDPAYRVNLASAVYASI
                     TVFLVFVAGYLLSRRVAAAFAAALAFGLGGTFWSQAVIAEIYTMNTLLIMLPIITLIL
                     WRERRERPRSDRYLLLACFLMGFALTNHLTSGLVLPASFLFVAAVDWRKLLDWRLALK
                     GAGLFVIGLLPYLYLPIRSSMNPPMNEADPSNFARFYEFVTGSDLNGVFGKFGPIELI
                     GRVIMYGQNLFADFHWGLVMVGIVGFMAMLVRDKAMAVLIGFLYFGWLAHALEFGIFD
                     TKLYFIPTYLMLSLAMAVGFGFLVRAVEDVFENRRRAVAGAGIAVVSALIALYPLVGI
                     GQKYEQYDMSDDYKGQEILDAVIHKAKHGGTVLHHRSGLWYMVLVEKKRPDLRIIDPW
                     YPSWKRETDIVWPDDIDLVTTNLRYGTNDYTGVATAKEAAKRGPVYILDQDSAGPHNF
                     IEAGFKIVRVEKGLLYELVPPGGKQYTSEKESES"
     gene            complement(4529..5515)
                     /locus_tag="VFJ72_06235"
     CDS             complement(4529..5515)
                     /locus_tag="VFJ72_06235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET7479096.1"
                     /translation="MRAWQVHELGDPKSALNLVEAPEPQPGNGEVVVEVEAAALNFFD
                     ILLCKGEYQEKPDLPFTPGAEVAGTIVGAGEGTDLQPGQRVIATPPLPRGGLAERVAA
                     PISGVFPIPDSMPFESAAATHIVYQTAHFALHRRAALRKGETVLVHAGAGGVGSAAIQ
                     LAKAAGASVIATAGGPEKIEVCRGLGADVTVDYREDNFIEVVKEATEGRGADIVFDPV
                     GGDVFDGSRRCVAFEGRIVVIGFAGGRIAEAPTNHLLVKNYSVVGLHWGLYARVMPHL
                     IPETHQELMRLYEAGRIDPLIYKTVSFEEVPDALDLLGSRKTYGKLVTRPGD"
     gene            5621..6283
                     /locus_tag="VFJ72_06240"
     CDS             5621..6283
                     /locus_tag="VFJ72_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HET7479097.1"
                     /translation="MLRVRDSMTREVITLGPEASAAEAWSLCQEHKIRHLPIVEEGRL
                     VGLVSDRDLRDVRGGGEDKETDSPRWVRVGEIMTRQIVTIHPLDTIDHAAREIYDRKI
                     GSLPVVADGELVGIITSADMMRTLIELVGAHGVGTWVEVEVPNEPGQLANLTDVVRDR
                     KVNIGSVFLGPASRETNRVIVLRLETTNPSGIVETLKDAGYNVTTVESSVAASVQSPY
                     EE"
     gene            complement(6296..7204)
                     /locus_tag="VFJ72_06245"
     CDS             complement(6296..7204)
                     /locus_tag="VFJ72_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7479098.1"
                     /translation="MKVLVTGARGKVGQATVAALQEAGHDVRATDILPPVFERPGEDE
                     ADYFQADLTDAGDAFAVVRGAEAVVHAAAIPEPTKNPPHRVFHNNLMATFNVLEAAIR
                     FGVPRFVNISSETVPGFFFPEREFLPDYVPVDEEHPIRPQDPYATSKHFGEQLMDAAV
                     RRSDIRAISIRPCWVQHEGNYERNLGPQVRDASVLSPNFWSYIDVYDLADAIVLAAES
                     DLPGHEVFYIASPDNVGSRPFEKTVREHYGGDIEIKPLSREDASGISCAKAEKMLGYA
                     PKRSWNDYLDENGKLKPGVEGLFSGS"
     gene            7296..8348
                     /locus_tag="VFJ72_06250"
     CDS             7296..8348
                     /locus_tag="VFJ72_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7479099.1"
                     /translation="MRIVVVGATGNLGTSVLRSLAREPAVDSVLGLARRRPGLSMPKV
                     EWEEADVTRDDLAPHFRGADAVVLLAWIIQPSRDLNRQWMVNVEGSTRAARAVKEAGV
                     PALLYASSVGAYSPGPKDRAVDESWPTGGIPTSYYGRHKAEVERRLDRFEREAPGVRV
                     VRMRPGLVFKREAAEGIRRLFAGPFFPGMLANPRLINFIPDIEDLRSQIVHSYDVGEA
                     FRLALTREVRGAFNLATEPVLDAPEIGRILNARPVPLSSRVARGAADLSWRLRLQPVP
                     PGWLDLSRQVPIMDTTRARDELGWTPRYGAEETLLEVLEGLRTGTGLDTPPLSPKTGG
                     PLRVREILTGVGGREP"
     gene            8357..9514
                     /locus_tag="VFJ72_06255"
     CDS             8357..9514
                     /locus_tag="VFJ72_06255"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HET7479100.1"
                     /translation="MWHGKEDVRVDNVPDPIIEEPTDAIIRITSTAICGSDLHLYKVL
                     APLMREGDIIGHEPMGIVEEVGTGVEHIKPGDRVVVPFNISCGHCYFCDQHLYSQCET
                     TRDTGKLAQASSLLGRGKGASLFGYTHVYGAVPGGQAEYLRVPQAHFGPIKVPEGPPD
                     ERFLFLSDVLPTAWQAVEYADTPEGGTLGVWGLGPIGQMCARIAMQQGVGRVIGVDTV
                     PERLSLAAKYGVETVDFNAVDRVRDVVLELTGGRGVDAGIDAVGMEASGSAIGSILQA
                     TKLQLDRATALRECMSSIRRGGTLSISGVYAGPIQAFQLGDLFDMQIQVRMGQANVRR
                     WVDDIMPLLGDDDPLGTEDLKTHEMPLEEAPRAYEIFQKKQDGAIKCVLKP"
BASE COUNT         1574 a         3034 c         3298 g         1632 t
ORIGIN      
        1 gcccgctgcc cgttcgggat gccgagcgtc atcgaaaacg agcggcacag gaacatgccg
       61 acgagcttct ggatcacctg caagaccctc gacgccgcca tcgcccggat agagtccacc
      121 ggcggcgtcc gggaagccca ggagcacctg ggcgaagaga agatagaaga gattcacacc
      181 gcccacaaga ccgcctacgg cacccgcgtc gcgggcgtgc gggagggcgg ctacgtaaaa
      241 tgcctccacg ccttcaccgc tctccacctc gcaggcgcca tcccgaaccc tgtcgctgaa
      301 tggaccctcc agcgactaca aaactcctat ccccaggacc gctgctgcac ggccggggag
      361 cccgaaaact agctgctata ctcccggatg agtgcggggg cgtggcggaa tggcagacgc
      421 aacggactta aaatccggtt cctgaaaagg agtgtgggtt cgagtcccac cgcccctact
      481 gacactcttg aggaggatct gcccatgatc accgcccacg ccgacatcgt tggaagtctg
      541 ctacgtccac cggaattgtt agaagcgcga gaactctacg ctgccggtgg gatgtccgcc
      601 gctgatttca aggccgtaga agaccgggcg gttgactggg ccctcgggct tcaggagcgg
      661 gcggggatgc cggtggtcac ggatggcgag atgcgccggg agtcgttcca gagccagctc
      721 gtggaggcgg tggagggttt cgcgggccac acgctggatg cctggctctg gggcgagtgg
      781 cacggggacg agtcggtggg ggacaagact accgagcgcc ccgcggcgct tggcgtggtg
      841 ggtaagttgc gccgccggag gcacctgtcg gtggaggagt tcgtgtacgc gagggcgcgc
      901 acggaccgca ccatcaaggt cacgctgccg agcccgagca tgtacgcgaa cctctggtcg
      961 ccggagcttt cgagagacgc ttatccgacg ctggacctgt tcctggagga cgtggcggag
     1021 atactgcgtg aggaggtcga ggagctggcg cggctgggcg cagagtacat tcagctcgac
     1081 gcgccgcact atccgctgct gctcgacccg aagacccgcg agttctacga gcggcggggc
     1141 tgggacctgg accgctggct agagcgcggg atcgagctgg acaaccacgt catcggggag
     1201 catcccggcg tgacgttcgc gttccatctt tgccggggca accaggggag ccggtggctg
     1261 gtctcgggtt cctacgagcc gctggcgcgc cgcgtcttcg gcggcatacg ggccgggcgc
     1321 ctgatgctgg agtacgacga cgagcgttcc ggaggcttcg agccgctggc ccacgtcccg
     1381 gaggacaagg tcgccgtact cgggctggta acgaccaaga ccccccggcg cgagactccc
     1441 gaggaactgg agacgcgcat ccgggaggca gcggaatata ttccgctgga acggctggct
     1501 ctcagccctc agtgcggttt cgcaacctcg gtcatcggca acgccgtagg cgtcgaggac
     1561 gaggagcgga aactgcgcgt gatctccgag accgccggac gggtctgggg ctaacgccga
     1621 agccggcgcc gaagctaacg cagcgcacgc cgcgccatca gcgagccgga aattagtaaa
     1681 gccagaccgg ccgcgagcgg taacagcggt actcctccgg tatccgggag gatggagatc
     1741 tccgcggaag cgacagccgg cgtggccgcc ggagcttggc ctgcgtccga ttgcgacttc
     1801 tcacccgtct cccgggcggc taccggctct gtctcggccg cgttcgcctg attcccgccc
     1861 tgtccgctat cgccggcctc tgcgggagcg ctctccaccg cttgcgagtc agccctggcg
     1921 gcggtgcgag gcgcggtggc ctgcttcgtc gcggcggatt cagtagtcgc cggagcctcg
     1981 gtgtcggcgt actggtcgct ggcggcggtc tctgcggagg gctctgcgga gggctggctc
     2041 ttcgctacag ggtccgcgga ctgtttgacg gtgtattgat ccgccgccgc ggtctcctta
     2101 cggcccgatg ccgtgctatc cggggacgta tcagaggtcg tctccggcgc tgcctgggtg
     2161 gtgacatccg gcgcctccgg cgcgggagtc ttggcggggc tggtagcatc cgcggcgccg
     2221 ttggacgatt ctccgccgcc gctggccccg ccggccccgc cgtttgaggc ggaggggtcc
     2281 acgtaggcgc cgaagtcggt cgtccagtaa ggggtgccgt tcacccacac gagcccgatg
     2341 ccgatgacct tataggggcc gagcatgttc gtgttgtggc cgggcgagat gcgccaggcc
     2401 tcgaagacct catcggcggt cgtctgtccg taggcgaggt tctcggcggt gtaggtgttg
     2461 tagtcgtagc cctcctgggc gatccgttcg ggatgtccgg agcctatcgg gtagtaggag
     2521 cttccctcgg tggtgtgaga gaagaagccg taggtgccca tatcttcgga gtgatgctcg
     2581 gatgcgacgg agagcggggc ggagagcagc agcggctcaa ggccgttctg ctggcggtac
     2641 tggttgatca actccaggaa ccgcagctct tgtggttcgt aggattgggc ctgggctata
     2701 ccggccgggc cgaccacgaa catcaggctc aagacggcga taaagagcca ccgcgctcgt
     2761 cgcatatttt ccccctccag gttgtatgtg accggacgtg ccgtccggca ccatgcaggg
     2821 ccgcagttta gcgagatgcg ccgccgtgtc aagcgggacg cgcacgggcc agacgagtta
     2881 actctatgta taattctctg ccggtgaaag actcccgcaa agagagcgac agcagccttc
     2941 gtagtgtggc gttcgtggcg ctcttcggac tggcggccgc ggtgttcgta ttcgtggtgt
     3001 atgtcaggac gctcgccccg acggtgctct actatgaccg tcccatactg ctcgactcgg
     3061 tgatgctcca ggcccaggcg acggtactgg ggatcacggg cccgacgggg gaacctacgt
     3121 gggtcatgct tacgcatctc ttcaccaagc tgcctttcgg ggacccggca taccgggtca
     3181 acctcgcctc ggcggtctat gcttccataa ccgtcttcct cgtcttcgtg gccgggtatc
     3241 tactcagccg ccgggtcgct gcggcgttcg ctgcggcgct cgccttcggg ctcggaggga
     3301 cgttctggag ccaggccgtc atcgcggaga tatacacgat gaacacactc ctgatcatgc
     3361 tccccatcat caccctgatc ttgtggcgcg agcggcgcga gcggccccgc agcgaccgat
     3421 acctgctact tgcctgcttc ctgatgggat tcgccctgac caaccacctc accagcggtc
     3481 tcgtcctgcc cgcctccttc ctgttcgtcg ctgccgtgga ctggcgaaag ctcctcgact
     3541 ggaggcttgc cctgaagggc gccggactct tcgtgatcgg gctactgccg tacctatact
     3601 tgccgatccg gtcgtccatg aacccgccga tgaacgaggc ggacccgtcg aacttcgccc
     3661 gcttctacga gttcgtgacc ggaagcgacc tgaacggcgt cttcggcaag ttcggaccca
     3721 tcgagcttat cgggcgggtc atcatgtacg ggcagaacct cttcgcggac ttccactggg
     3781 ggctggtcat ggtcgggata gtcggcttca tggcgatgct cgtccgggat aaggcaatgg
     3841 ccgtgctgat cggcttcctg tacttcggat ggctggcgca cgctctggag ttcgggatct
     3901 tcgatacgaa gctctacttt atccctacct acctgatgct ctcgctcgcg atggccgtgg
     3961 gcttcgggtt cctggtgcgg gccgtggaag acgtcttcga gaatcgccgg cgggcagtgg
     4021 cgggagcggg catagccgtg gtctcggcgc tgatcgcgct ctatccgctg gtcgggatcg
     4081 ggcagaagta tgagcagtac gatatgagcg acgactacaa gggacaggag atcctggacg
     4141 ccgtcatcca caaggcaaag cacggcggga cggtgctgca tcaccggagc gggctctggt
     4201 acatggtcct cgtcgagaag aagcggcccg acctgcggat catagacccc tggtaccctt
     4261 cctggaagcg cgagacggac atcgtatggc cggacgatat agacctcgtt acgacgaacc
     4321 tgcgttacgg caccaacgac tataccggcg tcgccacggc gaaggaggcg gccaagcgag
     4381 gccccgtcta catcctcgac caggacagcg ccggcccgca caacttcatc gaggcggggt
     4441 tcaagatcgt gcgggtcgag aaggggcttc tctacgagct cgtgccgccc ggcggtaaac
     4501 agtacacgag cgagaaagag tccgagagct agtcgcccgg acgggtgacg agcttgccgt
     4561 aggtcttgcg gctccccagc aggtccagag cgtcggggac ttcctcgaag gagactgtct
     4621 tgtagatcag gggatctatc cgtccggctt cgtagagccg cattagctcc tggtgcgtct
     4681 ccggtatgag gtgcggcatg accctcgcgt agaggcccca gtgcagcccg acgacggagt
     4741 agttcttgac gagcaggtga ttggttgggg cctcggcgat cctgcctccc gcaaacccta
     4801 ttactacgat tcggccctcg aacgccacgc agcggcgcga gccgtcgaag acgtcgccgc
     4861 ccacggggtc gaagacgatg tccgctcccc ggccctccgt cgcctccttg acgacctcga
     4921 tgaagttgtc ttcccggtag tctacggtca cgtccgcgcc gagcccgcgg cagacctcga
     4981 tcttctccgg cccgcccgcc gtcgcgatga cgctggcccc cgccgccttc gcgagctgga
     5041 tggcggcgga tcccacgccg cccgcgcccg catgcaccag caccgtctcg cctttgcgca
     5101 aggccgcccg ccggtgcagg gcgaagtggg ccgtctgata gacgatgtgc gtcgccgccg
     5161 cgctctcgaa cggcatcgag tccgggatgg ggaagacgcc ggagatgggc gcggccaccc
     5221 tctcggcgag accgccgcgg gggagcgggg gcgtggcgat gacccgctgg ccgggctgca
     5281 ggtccgtgcc ctcaccggct cctacgatgg ttcccgcgac ctcggcgccg ggcgtgaacg
     5341 ggaggtccgg cttctcctga tactcgccct tgcagagcag gatgtcgaag aagttcaggg
     5401 ccgccgcctc gacctcgact acgacttcgc catttccggg ctgcggttcg ggcgcctcga
     5461 caaggttcag ggcgctcttc gggtctccaa gctcgtgtac ctgccaggct cgcatgtttc
     5521 tccctccgat ctccgtcagg cgcgagctta ccacagcgcc gccgtcgcca ggatggtaag
     5581 ctccggtatc ggaaaaggaa ggacgaccag gaggataagc atgctacgag taagagattc
     5641 catgacccgc gaggtcatta cgctgggacc ggaggcgagc gcggccgagg cgtggtcact
     5701 ctgccaggag cacaagatac gccatctgcc catcgtggaa gaggggcggc tcgtcgggct
     5761 ggtctcggac cgggacctgc gggacgtgcg gggaggggga gaagacaagg agaccgactc
     5821 cccgaggtgg gtgcgcgtcg gagagatcat gacgcgccag atcgtcacca tccatcccct
     5881 cgatactatc gaccatgcgg cgcgcgagat ctacgaccgc aagatcggct ctctccccgt
     5941 cgtcgccgac ggcgagctgg tcggcatcat cacctccgcg gatatgatgc ggaccctgat
     6001 cgagcttgtg ggagcgcacg gcgtcggcac ctgggtcgag gtggaggtcc cgaacgagcc
     6061 gggccagctc gcgaacctga ccgacgtggt ccgggaccgg aaggtcaaca tcgggagcgt
     6121 cttcctcggc cccgctagcc gcgagacgaa ccgcgtgatc gtccttcggc tggagacgac
     6181 gaacccttcc ggcatcgtcg agaccctcaa ggacgccggc tataatgtga ccacggtcga
     6241 gtcctccgtc gcggccagcg tacagagccc ctacgaggag tagaggccgg gacttctagg
     6301 aaccggagaa cagcccctcg acgcccggct tgagcttccc gttctcgtcc aggtaatcgt
     6361 tccaggagcg tttcggggcg tatccgagca tcttctccgc cttcgcgcag gagatgccgg
     6421 atgcgtcctc gcgggagagg ggctttatct cgatgtcgcc gccgtagtgc tcgcgcacgg
     6481 tcttctcgaa tggccggctc ccgacgttgt ccggtgaggc gatgtagaag acctcgtggc
     6541 cgggtagatc ggactcggcg gccagcacga tggcatccgc caggtcgtag acgtctatgt
     6601 agctccagaa gttgggagag aggaccgagg cgtcgcgcac ctgcgggccg aggttccgct
     6661 cgtagttgcc ctcgtgctgc acccagcacg gccggatcga gatggcccgg atgtccgagc
     6721 gtcgcacggc ggcgtccatt agctgctcgc cgaagtgctt ggaggtcgcg taggggtctt
     6781 gcggacggat cgggtgctcc tcgtccaccg gcacgtagtc cggcaggaac tcccgctccg
     6841 ggaagaagaa gccgggcacc gtctcgctgg agatgttcac gaaacgcggc acgccgaacc
     6901 ggatcgccgc ctccagcacg ttgaacgtgg ccatcaggtt gttgtggaag acccggtgcg
     6961 gcgggttctt ggtcggctcc gggatggccg ccgcgtggac cacggcctcc gcgccgcgca
     7021 ccacggcgaa ggcgtcgccc gcgtccgtca ggtcggcctg gaagtagtcc gcctcgtcct
     7081 cgccgggacg ctcgaagacc ggcggcaaga tgtccgtcgc ccgcacgtca tggccggcct
     7141 cctgcaacgc cgcgaccgtc gcctgcccga ccttgccccg cgctccggta accaggacct
     7201 tcatctcgcc ctcctgtctg tgtgaacagt ccgggcgatg gtataccacc gggagcggcg
     7261 tttcgcccgc gagaggggcg ggtaaagggc cgcgcatgag aatcgtggtg gtgggggcaa
     7321 cgggcaacct cgggacgagc gtgctgcgct cgctcgccag ggaacctgcg gtggattccg
     7381 tactcgggct ggcgcgccgc cgtccgggcc tctccatgcc gaaggtcgag tgggaagagg
     7441 cggatgttac gcgggacgac ctcgcgccgc atttccgggg cgcggacgcg gtggtgctcc
     7501 tggcctggat catccagccc tcccgcgacc tgaaccggca gtggatggtg aacgtcgagg
     7561 ggagcacccg cgccgcgcgg gccgtgaagg aggccggcgt gccggcgctc ctgtacgcct
     7621 cttcggtcgg cgcgtactcg ccggggccga aggaccgggc cgtggacgag agctggccga
     7681 ccggcggcat cccgacctcg tactacgggc ggcacaaggc cgaggtcgag cggcggctgg
     7741 accgcttcga gcgggaggct ccgggggtgc gggtcgtcag gatgcggccg gggttggtct
     7801 tcaagaggga ggccgcggaa ggcatccggc ggctcttcgc cgggccgttc ttccctggca
     7861 tgctcgccaa ccccaggctc ataaacttca tcccggacat cgaggacctg cggtcccaga
     7921 tcgtccactc ctacgacgtg ggggaggcgt tccggctggc gctcacgagg gaggtccggg
     7981 gcgcgttcaa cctggctacc gagccggtgc tggatgcgcc ggagatcggg cgcatattaa
     8041 acgcccgtcc cgtgccgctc tcctcacggg tagcgcgggg cgcggcggat ctctcgtggc
     8101 ggctcaggtt gcagccggtg ccgccggggt ggctggacct ctcgcggcag gtcccgatca
     8161 tggacacgac ccgcgcgcgc gacgagcttg gctggactcc ccgctacggc gcggaggaga
     8221 cgctgctgga ggtgttagag ggactgcgga cgggaacggg gctcgacacg ccgccgctgt
     8281 cgccaaagac cggcgggccg ctgcgggtcc gcgagatcct caccggagta ggagggagag
     8341 agccgtaaaa gctgtagtgt ggcacggcaa ggaagacgtg cgggtggaca atgtcccaga
     8401 cccgatcatc gaggaaccca ccgacgcgat catccgcatc acctcgaccg ccatctgcgg
     8461 ctcggacctg cacctttaca aggtgctcgc cccgctcatg cgcgagggcg acattatcgg
     8521 gcacgagccg atgggcatcg tcgaggaggt cgggaccggc gtggagcaca taaagcccgg
     8581 cgaccgggtc gtggtgccgt tcaacatttc atgcggccac tgctacttct gcgaccagca
     8641 cctctactcc cagtgcgaga cgacacgcga caccggcaag ctcgcccagg caagcagtct
     8701 gttaggacgc ggcaagggcg cgagcctctt cggctacacg cacgtctacg gcgctgtccc
     8761 cggcgggcag gcggagtacc tgcgcgtccc gcaggctcac ttcggcccga taaaggtccc
     8821 cgaaggcccg ccggacgagc gtttcctgtt cctctccgac gtgctgccga ccgcctggca
     8881 ggccgtcgag tacgcggaca ctccggaggg cgggacgctg ggcgtctggg gtctcggtcc
     8941 tatcgggcag atgtgcgccc ggatcgcgat gcagcagggc gtgggacggg ttatcggggt
     9001 ggataccgtt cccgagaggc tctcgctggc ggcgaagtac ggcgtggaga cggtcgactt
     9061 caacgccgtg gaccgggtgc gggacgtggt gctggagttg accggtggcc ggggcgtgga
     9121 cgccgggata gacgctgtcg gcatggaagc tagtgggtcg gccattggtt cgatccttca
     9181 ggcgacgaag ctacagctcg accgggcgac tgccctgcgg gagtgcatgt catccattcg
     9241 gcgcggcggg acgctctcta ttagcggggt gtacgccggg ccgatccagg cttttcagct
     9301 aggagacctc ttcgacatgc agatacaggt ccgcatggga caggctaacg tgaggcgctg
     9361 ggtggacgac atcatgccgc tcctgggcga cgacgacccg ctggggaccg aagacctcaa
     9421 gacgcacgag atgccgctcg aagaggcgcc gcgcgcttat gaaatcttcc agaagaagca
     9481 ggacggcgcg atcaagtgcg tcctgaagcc gtagcgcggt aggcgcgtgg cagccggg
//
LOCUS       DASSMB010000096        10668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1158914, whole genome shotgun sequence.
ACCESSION   DASSMB010000096 DASSMB010000000
VERSION     DASSMB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10668
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158914"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFJ72_06260"
     CDS             <1..814
                     /locus_tag="VFJ72_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7479101.1"
                     /translation="YVGLCTGAALVGMRPIAEIQFSDFITHCMDQLVNQAAKLRYMFG
                     GEAEVPLVVRTPGGAGTGAAAQHSQSLEAWFIHVPGLKVVMPSTPYDAKGLLLAALDD
                     PNPVIFYEHKLLYNRKGEVPEEPYRIPLGEASVSREGEDVTVVAAGLVHRYALEAAEA
                     LADDGVEAEVVDLRTLSPLDHATVARSVEKTGKLVVVEEDVKTAGWGAEVVARIVEGE
                     SFYALDRAPARVAGADVPIPYNKNLEAHIRPSPEKVVEAVRSLMDARVGAYS"
     gene            815..2092
                     /locus_tag="VFJ72_06265"
     CDS             815..2092
                     /locus_tag="VFJ72_06265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET7479102.1"
                     /translation="MATELKVPSLGMDMEEANIVRWLVEEGAEVEKGQPILEIDTDKT
                     SFEIESPATGIVRGIQGAEGATVPVGATLAFIAAPGEQAPASGADGRAKADEPPAQQT
                     RPDAQSSPAPDAQADAQADAQAPQSNGDRRRLRASPAARRAAEERGLRLEDVAGSGPG
                     GRVYLSDVLEADVRQDAPTPEAPATVAPAPESISPTPAVADGSRREPLSRIRRLGAER
                     TAKSFREVPHFYLTRDLDVERLLDLRERLKERMDPAPSVTDLLALAIARTLKAHPRLN
                     GRYVEGELEVRESVNLGIAVATGDGLVVPVLKNADKLILKELVPAAKGLVTRARDGRL
                     APEELSGGTFTVSNLGMIEIDSFDAIINAPEAAILAVGRVRTVPEWRDGEWLPRRVMS
                     GTLSVDHRVADGADGARFLMDLQAALTDWELLL"
     gene            2112..2459
                     /locus_tag="VFJ72_06270"
     CDS             2112..2459
                     /locus_tag="VFJ72_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479103.1"
                     /translation="MKVENDAVLTCSACGIEGPHELLYLAEHLCASRCINCGKTQVYS
                     GHIYTEYARDLAERTTHLPFRFAGDVLRHPTELVKWPFKALGKPWGLLREVNQVTAFE
                     RSRRYPTPGRRVG"
     gene            complement(2474..4105)
                     /gene="ptsP"
                     /locus_tag="VFJ72_06275"
     CDS             complement(2474..4105)
                     /gene="ptsP"
                     /locus_tag="VFJ72_06275"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET7479104.1"
                     /translation="MQEHARLTGIGASEGVAVGPVFVHRPGELKPDRERIPEGEVDAE
                     LERFKASVAVVVERLAETADELRDAGNESEAGIFDAHAEMAEDPDLQEGVEERVRDLQ
                     SPEAAILEVGEEYAGMFAAMDDEYMAARADDVRDVAAQIASQLMGREATGLASLGTPS
                     VVLASSLAPSDTARIPKGMALGFVTAEGSKTSHVSIMARSMGIPAVVGVGPRLEGVFD
                     AKVAAVDGSEGYAVADPDPETVEGFEAKMRTMAEERTALEKYRYVEARTSDGRRIEVA
                     ANLGSAKDAEDAISQGAEGVGLFRTEFLFMERDQLPTEEEQYESYSAVTRAFGERPVV
                     IRTLDVGGDKDLPGVDQPVEENPFLGWRGIRMCLDVPELFKPQLRALLRAGAHGNLKI
                     MFPMVADVSEIRAAKKILDECRDELESEGTEFGPVSVGVMVETPAAAILAEALAPEVD
                     FFSIGTNDLVQYTLAADRGNERLRHLQSADHPAVLKLIGLTCQAARDASIWVGVCGEA
                     AGEPALIPKLVELGVTELSMSAPSIARAKKIVSEL"
     gene            complement(4110..4373)
                     /locus_tag="VFJ72_06280"
     CDS             complement(4110..4373)
                     /locus_tag="VFJ72_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HET7479105.1"
                     /translation="MVEREVTVVPKEGLHARPAAMFVKEAKRYTSTDIVVVKGETEAN
                     AKSSLKLMTLGAKHGDTLIIRADGDEEEAAADALAALISQEEE"
     gene            complement(4470..5936)
                     /locus_tag="VFJ72_06285"
     CDS             complement(4470..5936)
                     /locus_tag="VFJ72_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HET7479106.1"
                     /translation="MKFIGVTACPTGIAHSAMAAEALETAAKEKGHEIWIEIHGASGA
                     DFVDPQVIRDADAVMIASDATVAQRDRFAGKPTVEVRLREAIDKPGDVVDKAVAAAER
                     GASAAPAGAAAEEEDDANATIPTGAAGGGWGSEVRRWLMTGVSYMIPFVVAGGILIAL
                     SFALGDTVKVTDSDVYKDFSIPALLFKIGATAFGMLVPILAGFIAYAMADRPGIAPGV
                     VGGLIANDIGAGFLGGIAAGLLAGAVIILLKKIKVPGGFGRLMPILIYPLVGCLVVGT
                     LMIVVLGQPIASATEGLQAWLKGLQGTNAILLGIILGSMMAFDMGGPVNKAAYAFAIA
                     GLAGGAYGPMAAVMAAGMTPPLALALANLIRPSLFTPAEQRASQANWVLGASFITEGA
                     IPFAAADPLRVIPACMIGSALTASMSLAFGATLRAPHGGIFVIGLIGHWPLYLLAIAV
                     GTVVSAVLVVVFKSLGSRAAEQTATADSPQASRQAARA"
     gene            complement(5991..6455)
                     /locus_tag="VFJ72_06290"
     CDS             complement(5991..6455)
                     /locus_tag="VFJ72_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET7479107.1"
                     /translation="MSPTIDDISNPNLVALDLDLADRWEAIDALADLLDRDGRLSDRD
                     AYVSAVRAREEATGGTAMEMGIAIPHAKSAGVSEAGFAFGRATSAVDFGDEEADLIFL
                     IAAPEGEANLHVTVLQQLARRLVHEKFRTALREARTPEEAFNLIREEVSISK"
     gene            complement(6452..7402)
                     /locus_tag="VFJ72_06295"
     CDS             complement(6452..7402)
                     /locus_tag="VFJ72_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HET7479108.1"
                     /translation="MIVTLTPNPSLDLTYELEKVLLGGVQRATEFSVEAGGKGINVSR
                     NLVANGVASRAVAPVGGPSGEQFLGLLEESGIELARVPVSGPVRANVTVVDREGVVTK
                     INAAGPRLSAVEVDLLLEETAAAGRGADWVAACGSLPPGAPVDLLARVVEAARESGCR
                     AVVDSSGAPLAAALKATPDVMKPNLEELSEIVDRRLTTFGDVLDAAEEVRAAGVSTLF
                     VSLGADGAVLVDGSGARHADTPPFTPRSTVGAGDALLAGFLAAGGEGEAALVEGVAWG
                     AAAARLPGTRTLTPKDVDRESVRVHNDIDRERQLKGEAPR"
     gene            complement(7399..8184)
                     /locus_tag="VFJ72_06300"
     CDS             complement(7399..8184)
                     /locus_tag="VFJ72_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET7479109.1"
                     /translation="MYEFREQPIYAEERHGFILDLARAEGRVEVSALADSFGVSAETI
                     RRDLAALERQGMLRRTHGGAVPMERLRFEPEVAERLATMTEEKARIARAAVAFVPSRG
                     TILLDAGTTTGALADLLPGGRDLTVVTNGLPIASMLAAHSSMTVLMAGGRVRGKTLAA
                     VDDLAARFIEDYAPDVAFVGTNGMTVARGLMTPDPAEATVKRAMIRNGRRVVVLADHT
                     KVGQEHFVRFAQTGEVDVLVTDSGLGDDAAQEFEAAGVEVVRA"
     gene            complement(8419..9042)
                     /locus_tag="VFJ72_06305"
     CDS             complement(8419..9042)
                     /locus_tag="VFJ72_06305"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET7479110.1"
                     /translation="MVLQKKTLGATERDEEARSAFRERLRGVEAERLVFVDESSTNIA
                     LTPRYARSPRGERAYGKAPRNWGKNVTLIEEISVTQGMGPSMSIEGSSDTESFSLYMR
                     NILAPQLKRGQIVLMDNLSVHRSKWVRDLIEAQGCHLWLLPSYSPDLNPIEEAFSKVK
                     NLLRKAKARTLETLFEATAQALEAVSAVDARGYFEHCGYSTPQDHSL"
     gene            complement(8967..9377)
                     /locus_tag="VFJ72_06310"
     CDS             complement(8967..9377)
                     /locus_tag="VFJ72_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HET7479111.1"
                     /translation="MEIYSKDLRIRAVAAVERGMPRREVVDTFRISLTTLKRWLRMSR
                     EGNDLSPGTSTGRRRRILATLEEKKALWKQLEENDDATLERHCELWEERTGVWVSIST
                     MSRAIRHKLGWSYKKRRWVPPSGTKKLEVLSENA"
     gene            complement(9781..10653)
                     /locus_tag="VFJ72_06315"
     CDS             complement(9781..10653)
                     /locus_tag="VFJ72_06315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7479112.1"
                     /translation="MAANISPVMGHYIDVKVGGDTYEVFFLENGEGIPLLCQHTAGAH
                     NHQYRNLLRDPEVTKNFRVIVYDLPYHGKSDPPHGKEWWKEQYDLTSGFFQEFVLNLA
                     DALDLDRPVFMGSSMGGVLCLYLARNHPEKFRALIALEAADFTPGFYLDWWARPEIND
                     GELASSVVEGLMAPQTPELDRRLTMWYYSQAAPGVLKGDLYFYPMEHDMREKVSEIDT
                     SMVPLYLLTGEYDYLSTPEQSQATAEKISGARFEAMREIGHFPMSEDHETFMGYVGPI
                     LQEIRSGDKVAGTA"
BASE COUNT         1762 a         3685 c         3348 g         1873 t
ORIGIN      
        1 ctacgtcggg ctctgcaccg gcgcggcgct cgtcgggatg cgccccatag ctgaaataca
       61 gttctcagac ttcatcaccc actgcatgga ccagctcgtg aaccaggctg ccaagctccg
      121 gtacatgttc ggcggcgagg cggaggtccc gctggtcgtc cgcacccccg gaggcgcggg
      181 aaccggagcc gcagcccagc actcccagtc cctcgaagcc tggttcatcc acgtgccggg
      241 tttgaaggtc gtcatgccgt caacgcccta cgacgcgaag ggcctgctcc tcgccgctct
      301 cgacgacccg aaccccgtga tcttctacga gcacaagctc ctctacaacc gcaagggcga
      361 ggtccccgag gagccgtacc gcatcccgct gggtgaggcg agcgtcagcc gcgagggcga
      421 ggacgtcacg gtcgtggccg ccggtctcgt gcaccgctac gcgctggagg ccgccgaagc
      481 tctcgcggac gacggcgtgg aagcggaggt cgtggacctc aggacgctct cgcccctgga
      541 ccacgctacc gtcgcccgct cggtcgagaa gaccggcaag ctcgtcgtag tcgaggagga
      601 cgttaagacc gccggctggg gcgcggaggt cgtggcgcgg atcgtggagg gcgagagctt
      661 ctatgctctc gacagggcgc ccgcgagggt ggcgggcgcg gacgtaccga tcccctacaa
      721 caagaacctg gaggcgcaca taaggcccag cccggagaag gtcgtcgagg cggtgcgctc
      781 ccttatggac gcccgcgtgg gagcgtactc ctagatggct accgagctga aggtcccatc
      841 gctgggcatg gacatggaag aggcgaacat cgtccggtgg ctggtagagg agggcgccga
      901 ggtcgagaag ggccagccga tcctggagat agacaccgat aagacgtcct tcgagataga
      961 gtcgccggcc accgggatcg ttcgcggcat ccagggcgcc gaaggcgcga ccgtaccggt
     1021 cggcgccacg ctcgccttca tcgccgctcc cggcgagcaa gcccccgcat ccggcgcgga
     1081 cggaagggcg aaggccgacg aaccgcccgc gcagcagacg cggccggatg cgcaaagcag
     1141 cccggcccca gatgcgcaag cagatgcgca agcagatgcg caagcgccgc agtccaacgg
     1201 cgaccggcga cggctccggg cctccccggc ggcgcgacgg gcggcggagg agcggggact
     1261 gcgcctggag gacgtcgccg ggtccggccc cggcggacgg gtctacctct cggacgtgct
     1321 ggaagcggac gtaaggcagg atgctcccac gccagaggct cccgcgacgg tggctcctgc
     1381 gccagagtcc atctccccca ctcccgctgt cgcggatggt tcgcgccgcg agccgctctc
     1441 gcggatacgg cggctgggag cggagcgaac ggcgaagagc ttccgcgagg tgccgcactt
     1501 ctacctcacc cgcgacctgg acgtggagcg cctgctggac ctccgggagc gcctgaagga
     1561 gaggatggac cccgcgccct ccgtcacgga tctcctggcg ctcgccatcg cccgcacgct
     1621 caaggctcat ccgcgcctca acggacgcta cgtcgagggc gagctggagg tccgtgagag
     1681 cgtcaacctc gggatcgcgg tggctaccgg cgacgggctc gtcgtcccgg tcctgaagaa
     1741 cgcggacaag ttgatcctca aggagctggt gcccgcagcg aaaggtctcg taacgcgcgc
     1801 cagagacggc aggctggccc ctgaggagct atcgggcggc acgttcacgg tctcgaacct
     1861 gggcatgata gagatagatt ccttcgacgc catcatcaac gcgccggagg ctgctatcct
     1921 ggcggtggga agggtcagga ctgttccgga gtggcgggac ggcgagtggc tgccccggcg
     1981 cgtgatgtcg ggtacactct cggtggatca ccgggtcgcg gatggggctg acggcgcccg
     2041 gtttttgatg gacttgcagg ccgcgctcac cgactgggag ctgctactgt gaaggggatg
     2101 aaggggacgc cttgaaggtc gaaaacgacg ccgtgctcac ctgctccgcc tgcgggatcg
     2161 agggtccgca cgagctgctg tacctcgcgg agcacctttg cgccagccgg tgcatcaact
     2221 gcggcaagac ccaggtctac tcgggacaca tctacaccga gtacgcccgc gacctcgccg
     2281 agcggaccac ccacctcccc ttccggttcg ccggggacgt gctccgccac ccgacggagc
     2341 tcgtgaagtg gcctttcaag gcgctcggca agccctgggg tctgctgcgc gaggtgaacc
     2401 aggtgaccgc cttcgagcgg agccgccggt accccacgcc cggccgtagg gtcggctaga
     2461 gagccggcgg gagctacagc tccgagacga tcttcttagc ccgcgctatc gacggcgcgc
     2521 tcatcgagag ctcggtcacg cccagttcga ccagcttcgg gatcagggcc ggttctcccg
     2581 ccgcctcgcc acacacgcct acccagatgc tcgcgtcgcg cgcggcctgg caggtcagac
     2641 cgatcagctt caggacggcc gggtggtcgg cgctctgcag gtggcgcagg cgctcgttgc
     2701 cgcggtcggc ggccagggtg tactgcacca ggtcgttcgt cccgattgag aagaagtcga
     2761 cctccggcgc cagcgcttcg gccaggatag ccgccgccgg cgtctcgacc atcacgccca
     2821 cgctcaccgg gccgaactct gtgccctcgc tttcgagttc gtcgcggcac tcgtccaaga
     2881 tcttcttggc cgcccggatc tcgctgacgt ccgcgaccat ggggaacatg atcttcaagt
     2941 tcccgtgcgc gccggcccgc agcagcgccc ttagctgcgg cttgaacagc tcgggcacgt
     3001 ccaggcacat cctgatgccg cgccaaccca ggaacgggtt ctcttctacg ggctggtcaa
     3061 cgccgggcag gtccttgtcc ccgccaacgt ccagcgtgcg gatgaccacc ggccgctcgc
     3121 cgaacgcccg cgttaccgcg ctgtaggact cgtactgctc ctcctccgtc ggtagctggt
     3181 cccgctccat gaacaggaac tccgtgcgga agaggcccac accctcggcg ccctgcgaga
     3241 tcgcatcctc cgcgtccttc gccgagccca ggttggccgc gacctcgatg cggcgcccgt
     3301 cgctcgtgcg ggcctcgacg taccggtact tctccagagc tgtgcgctcc tcggccatcg
     3361 tccgcatctt agcctcgaac ccctcgaccg tctccgggtc cgggtccgcg accgcgtagc
     3421 cctcgcttcc gtccacggcg gcgaccttgg cgtcgaagac accctccaac cggggaccga
     3481 ccccgacaac cgccggtatg cccatcgagc gggccatgat cgagacgtgc gaggtcttcg
     3541 agccctcggc ggtgacgaag cccagagcca tccccttcgg gatgcgggcc gtgtcagaag
     3601 gggcgagact actcgccagg acgaccgacg gggtgcccag cgaagcaagc cccgtggcct
     3661 cgcggcccat cagctgggag gcgatctggg cggccacgtc gcggacgtcg tcggcgcggg
     3721 cggccatgta ctcgtcgtcc atcgcggcga acatcccggc gtactcctcg ccgacctcca
     3781 gtatggcggc ctccgggctc tggaggtccc gcacccgctc ctcgacgccc tcctggaggt
     3841 ccgggtcctc ggccatctcg gcgtgggcgt cgaagatccc cgcttcggac tcgttcccgg
     3901 cgtcacgcag ttcgtccgcc gtttcggcaa ggcgctccac gacgaccgcg accgacgcct
     3961 tgaaccgctc cagctccgcg tcaacttcgc cctcggggat cctctcccgg tcgggcttga
     4021 gctcgccggg acggtgaacg aacaccggcc cgacggctac tccttcggag gctcctatcc
     4081 ccgtgagcct ggcgtgctcc tgcatgacct cactcctcct cctgggagat cagggcggcc
     4141 agggcgtcgg cggcggcctc ctcttcgtcg ccatcggccc ggatgatgag cgtgtccccg
     4201 tgcttggcgc cgagggtcat cagcttgagc gaactcttgg cgttcgcctc cgtctcgccc
     4261 ttcaccacca cgatgtcggt cgaggtgtag cgcttggcct ccttgacgaa catcgccgcc
     4321 gggcgggcgt gcaggccctc ctttgggaca acggtcactt ctctctccac catatacggt
     4381 cctcgcttcc tttccccacg cttacaggat ctttagctgg tcccggacgc taacctgagc
     4441 gcgtccggga cctcttcttt ctaacaggtc taggcgcgcg ccgcctgccg ggacgcctgg
     4501 ggggagtcgg ccgtcgcggt ctgctcggcc gccctcgaac cgagcgactt gaacacgacc
     4561 acgagcacgg cgctcacgac ggtgccaacg gcgatggcga gcaggtacag cggccagtgg
     4621 ccgatcagcc ctatgacgaa gatgccgccg tgcggggcgc ggagagtcgc tccgaacgcc
     4681 agggacatag acgccgtgag cgcggagccg atcatgcagg ccggtatcac gcgcagcggg
     4741 tccgccgccg cgaatggtat ggcgccctcg gtgatgaacg aggctccaag gacccagtta
     4801 gcctggctgg cgcgctgctc ggccggagta aagaggctgg gccgtatcag gttggcgagc
     4861 gccagagcca gtggaggagt cattcccgcc gccatcaccg ccgccatcgg cccgtatgcg
     4921 cctccggcga gaccggctat cgcgaaggcg taggcagcct tgttcaccgg cccgcccata
     4981 tcgaaggcca tcatcgagcc gaggatgatg cccagcagga tcgcgttcgt gccctgcaga
     5041 cccttgagcc aggcttgcag cccctcggtc gcggaggcta taggctgccc caggaccacg
     5101 atcatgagtg tcccaaccac cagacatccg acgagcggat agatcaggat cggcatgaga
     5161 cgtccgaagc cgcccggcac tttgatcttc ttgagcagga ttatcacggc gccggcgagg
     5221 agcccggctg cgataccgcc gaggaagccc gcgccgatgt cgttggcgat gagccccccc
     5281 acgacacccg gagcgatgcc gggcctgtca gccatcgcat aggcgataaa gccggccagg
     5341 atcggcacga gcatcccgaa ggccgtggcg ccgatcttga agagcagggc cggaatcgag
     5401 aagtccttgt acacatccga gtcggtcacc ttaacggtat ctccgagggc gaaggagagc
     5461 gcgatcagga tgccgcccgc aaccacgaac gggatcatgt agctgacgcc ggtcatgagc
     5521 cagcgccgta cctccgagcc ccatccaccg ccggcagcgc ccgtcgggat cgtcgcgttc
     5581 gcgtcgtctt cttcctccgc cgcggcacca gcgggcgcgg cgctcgcgcc tcgctcggcg
     5641 gcggctaccg ccttgtccac cacatcaccg ggcttgtcta tcgcttccct gaggcgcacc
     5701 tccacggtcg gcttcccggc gaaacggtct cgctgggcga cggtcgcgtc ggaagcgatc
     5761 atgaccgcgt cggcatcccg gatcacctgc gggtccacga aatccgcgcc ggaggcgccg
     5821 tggatctcga tccagatctc gtgtcccttc tctttcgccg ccgtctccag agcctcggcg
     5881 gccatcgcgc tatgggcgat ccccgtcggg caggcggtca ctcctataaa cttcactgcg
     5941 ctatctccct ttcctcacct tttccttgac tatcgtgcct gttctttctg ctacttcgag
     6001 atggagacct cctcgcggat gaggttgaag gcttcctcgg gcgtcctggc ctcccgcagt
     6061 gccgtcctga acttctcgtg gacgagcctc cgggcgagct gctggagcac cgttacatgc
     6121 aggttcgcct cgccttcggg agcggcgatc aagaagatca aatccgcctc ctcgtccccg
     6181 aagtccacgg cgctggtggc gcgaccgaag gcgaagccgg cctccgacac cccggctgac
     6241 ttggcgtgcg ggatggctat gcccatctcc atcgcggtgc cgccggtcgc ctcttcccga
     6301 gcccggacgg ccgagacgta tgcgtcccgg tcgctgagcc gcccgtcgcg gtccaggagg
     6361 tcggcgagag cgtctatggc ctcccaccgg tcggcgaggt cgaggtcgag ggccacgagg
     6421 ttcgggttgc tgatgtcgtc tatggtcggg ctcatctggg tgcttctcct ttcagttgtc
     6481 tctccctatc gatgtcattg tggacccgca cggactcccg gtccacatcc ttcggcgtca
     6541 gggttctggt gccgggaagc cgcgccgccg ccgctcccca tgccaccccc tccacgagcg
     6601 ccgcttcgcc ctcaccgcca gcggctagga acccagcgag cagagcatca cccgcgccga
     6661 ccgtgctgcg cggcgtgaac ggcggggtat ccgcgtgccg cgcgccggac ccgtccacca
     6721 ggaccgcgcc gtccgctccg agtgagacga agagggtaga tactcccgcc gcccggactt
     6781 cttccgcggc gtcgaggacg tccccgaagg tcgtaagccg ccggtccacg atctccgaca
     6841 actcctccag gttgggcttc atgacgtccg gtgtggcttt gagggcagcc gcgagcgggg
     6901 ctccactgct gtccacgacg gcgcggcacc cggattcgcg ggcggcctcg acgacgcggg
     6961 cgagcaggtc cactggcgcg ccgggcggca gactcccgca ggccgcgacc cagtccgcgc
     7021 cgcgtccggc ggcggccgtc tcttcaagca gcaggtctac ctcgactgcg gacagcctgg
     7081 gaccggccgc gttgatcttg gttacaaccc cctctctatc aacgacggtc acgttcgcgc
     7141 gtaccggccc gctgacgggc acccgcgcga gctcgatgcc ggactcttcc aagagaccga
     7201 ggaactgttc cccgctcgga cctcctaccg gcgctacggc cctcgaagcg acaccgttag
     7261 caaccaggtt gcgggagacg ttgataccct tgcctccagc ctcgacggag aactcggtag
     7321 cccgctgcac ccccccgagc aagaccttct caagctcgta ggtgagatcc aggctcgggt
     7381 taggggtcag ggttacgatc atgcgcgcac cacctccact ccggcggcct cgaactcctg
     7441 cgccgcgtcg tcaccgagac cgctgtcggt gacgagcacg tccacctcgc cggtctgggc
     7501 aaaccgcacg aagtgctcct ggccgacttt cgtgtggtcc gccagcacca cgacccgtcg
     7561 cccgttccgg atcatggccc gctttacggt ggcctcggcc gggtccggcg tcatgaggcc
     7621 ccgcgcaacg gtcatgccgt tggtcccaac aaatgccaca tcgggagcat aatcctcgat
     7681 gaaacgcgcc gccaggtcat ccacggcggc gagcgtcttc ccgcgcaccc gcccgccggc
     7741 catcaatacc gtcatggaag agtgagccgc gagcatgctc gctatcggaa gcccgttcgt
     7801 gacgaccgtc aggtcccgcc ctcccggcag gagatccgcg agcgctccgg tagtcgtgcc
     7861 cgcgtcgagt agtatcgtcc cgcgagacgg cacaaacgct accgcagccc gcgcgatgcg
     7921 agccttctcc tccgtcatgg tcgccagacg ctccgcgacc tccggctcga accgcagacg
     7981 ctccatcggg accgctccac catgagtccg cctcaacatc ccctggcgtt cgagcgccgc
     8041 gagatcccgc cgtatagtct cggccgatac cccgaacgag tccgccaaag cggatacctc
     8101 aacccgcccc tccgcgcgag ccagatccag gataaacccg tgccgctcct ccgcatatat
     8161 cggttgttcg cgaaactcat acatttcccc tatagccctt tcccgtatat gtgggtctat
     8221 ttgactattt gattccgtat atacccgaat ttgtggttat acgcaagagc catcgggcat
     8281 aatttcaaga aatttctgta aaaagccgaa aaacgccgca atctgctaaa tgtaggcgaa
     8341 gactatcgtt actaaagtat ctagacatat gcccactgca tgttccgcaa cgacgcggcg
     8401 gtggatctag agcggttctc ataatgagtg gtcctgcggt gtactgtagc cgcaatgctc
     8461 gaagtagccg cgagcgtcga cggcgctgac cgcctcaagc gcctgcgcgg tggcctcgaa
     8521 gagcgtttcg agggtccttg ccttcgcctt tctaagcagg ttcttcactt tggagaaggc
     8581 ttcctctatg gggttgaggt ccggcgagta gctcggaagg agccacagat ggcatccctg
     8641 cgcctcgatg agatccctga cccatttgct cctgtgtacc gagaggttgt ccatgaggac
     8701 gatctgcccc ctcttcaact gcggagccag gatgttcctc atgtagagcg agaaggactc
     8761 cgtgtccgaa gacccttcga tgctcatgga aggtcccatc ccctgcgtca cgctgatctc
     8821 ctcgatcagc gtgacgttct tgccccagtt ccttggagcc ttcccgtatg ccctctctcc
     8881 cctgggcgac ctcgcgtagc gtggggtgag agcgatgttg gtcgaggact cgtccacgaa
     8941 caccaagcgc tccgcctcaa cccctctcag gcgttctctg aaagcacttc tagcttcttc
     9001 gtcccgctcg gtggcaccca gcgtcttttt ttgtaggacc agccgagctt atggcgtatg
     9061 gccctgctca tggtggagat cgacacccaa acccccgtgc gctcctccca gagctcgcag
     9121 tggcgctcga gggtggcgtc gtcgttctcc tcgagctgct tccacaaggc cttcttctcc
     9181 tctaaggtgg cgaggatgcg ccgtcttctg cccgtcgagg tgcctggcga aaggtcattg
     9241 ccctctctgc tcatcctcag ccagcgtttg agcgtcgtga gggagatgcg gaaggtgtct
     9301 acgacctccc ttctaggcat accccgctcg acggcggcca ccgccctgat cctgaggtcc
     9361 ttcgaataga tctccatgca cgacatcctt gcacaagacc actctattcg caaattgctc
     9421 taggctaccg actcgggttc atgttatgtc ccgcaaaata acgtgtcgca cagcaaaaga
     9481 tgaggttcat taaaccgcct tgcaggacat tcagcagctg gggaagtgac ggagacatgg
     9541 agcaatcgac cggtcgaagg cctcgtaaag aatttcaagc tgttgcggcg tcggcggtat
     9601 ggacgcgctg gtgtcctggc tgtccgagag cgaggatgcc ggcttgcgac aacgagacga
     9661 actcgagcca ggacgccggc tgctctcgtc accccaaccc gcgcaggtgg gtccttcgcg
     9721 ggcgggtcct tcgtgggcag caccgcgcct ctcccgcgta gtccccttcg aggccgatta
     9781 ttaggccgtg cccgccacct tatcgccgga gcgtatctcc tgaaggatcg gtccgacgta
     9841 gcccatgaac gtctcgtggt cctcgctcat cgggaagtga cctatctctc gcatggcctc
     9901 gaacctcgcg cccgagatct tctcggctgt tgcctggctc tgctcgggtg tcgagaggta
     9961 atcgtactca ccggtgagga ggtacagggg caccattgac gtgtcgatct ccgagacctt
    10021 ctctctcatg tcgtgctcca tggggtagaa gtagaggtcg cccttgagga cgccgggcgc
    10081 ggcctgggag tagtaccaca tggtcaggcg ccggtcgagc tccggggtct gcggcgccat
    10141 gagcccttcg accacggatg aggcgagctc tccgtcgttg atctcggggc gcgcccacca
    10201 gtccaggtag aagccgggcg tgaagtccgc cgcctccagc gctatgagcg ccctgaactt
    10261 ctccgggtgg ttacgggcca ggtacaggca cagcaccccg cccatcgagg agcccatgaa
    10321 cactggacgg tcgaggtcca gcgcatcggc gaggttcaga acgaactcct ggaagaagcc
    10381 gctcgtgaga tcgtactgct ccttccacca ttccttgccg tgcggagggt cggacttccc
    10441 gtggtagggt aggtcgtaga cgatcacccg gaaattcttg gtgacctcgg gatcgcgcag
    10501 taggttccgg tactggtggt tgtgggcccc ggcggtgtgc tggcagagaa gcggtatgcc
    10561 ctctccgttc tcaaggaaga acacctcgta ggtatctccg ccgaccttga catcgatgta
    10621 gtggcccatg acgggcgaaa tgtttgctgc catcagcgct ctcctctc
//
LOCUS       DASSMB010000097        15374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_273516, whole genome shotgun sequence.
ACCESSION   DASSMB010000097 DASSMB010000000
VERSION     DASSMB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15374
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273516"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFJ72_06320"
     CDS             <1..219
                     /locus_tag="VFJ72_06320"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564543.1"
                     /note="catalyzes the formation of protocatechuate from
                     4-hydroxybenzoate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HET7479113.1"
                     /translation="SGRTDLLDSYSETCLRRVWKASRFSWWMTSMLHRFHDDDEFRHR
                     LQVAELDYVTSSRAASTSLAENYVGLPM"
     gene            complement(235..417)
                     /locus_tag="VFJ72_06325"
     CDS             complement(235..417)
                     /locus_tag="VFJ72_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479114.1"
                     /translation="MKEREFKVVAVTLFGKSEVARYPTREQAEWRLRELRDEAERNPR
                     GYLQYIIVPVKAAEDE"
     gene            527..2149
                     /locus_tag="VFJ72_06330"
     CDS             527..2149
                     /locus_tag="VFJ72_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7479115.1"
                     /translation="MKGLVQDFQLTLPYILRRAEQLYGYREIVTRRPDKSFHRYTYAD
                     FVSRAKKLAVAMKKLGVQDGDRVGTLGWNTYQHLEAYFGVPSSGAVLHTLNPRLHEDD
                     LAYITDHAGDRVMLVNETLVPVFQKFRDRTKLEHAIVVTEGGEVPDGMLSYEDLLADA
                     DESEFEYPDFDEDQAAALCYTSGTTGRPKGALYSHRSTVLHSIMVSMGNTFALSEADC
                     MLPVVPMFHVNAWGMPYASVLVGAKIVMPGPHLDPQSLIEDFVQEGVTFTAGVPTVFL
                     ALLQALDKEPGKHDLSSIRAMAIGGSAAPKGVIKRFQDDHGLKVVHAWGMTEMSPIGS
                     VANLPSPVRELSEDEQLDYRAKQGYPVPFVEIRARGDEGLVPWDDRSMGELEVRGPWV
                     ASTYFNTSEGDDKFTDDGWFTTGDIVTIDKYGFIKIQDRSKDLIKSGGEWISSVELEN
                     ALMAHPAVSQAAVIAIPHEKWDERPLAAIVLKEGESVTEEELRDHLAKDFAKFWLPDA
                     YEFVEEIPMTATGKFQKLKLREQFEGYTTTGS"
     gene            2158..2910
                     /locus_tag="VFJ72_06335"
     CDS             2158..2910
                     /locus_tag="VFJ72_06335"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7479116.1"
                     /translation="MKVIGAGFGRTGTTSLKAALETLGFGPCYHMTEVFAHPRHASTW
                     DAAWRGEPVDWDAFLAGYEATVDWPACTFYRELMEEYPEAKVLLSVRDPERWYESVRS
                     TIYELSESFIRSPVFRAIFGLTSFFAFGGFLRSSWGDMVNEIILQGTFDGRLEDKAYA
                     MWVFERHNEEVKRSVPPDRLLVYEVKESWGPLCEFLGVPEPDAPFPRLNDGAQMQRAI
                     RTIRGLSFALPVTVILVPCAAALALLRRRARP"
     gene            2944..4695
                     /locus_tag="VFJ72_06340"
     CDS             2944..4695
                     /locus_tag="VFJ72_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET7479117.1"
                     /translation="MNETREIIVQKDVPAEMRDGTNLMSNVYRPNDEGDYPVLLTRLP
                     YGKDLPLGTSYLDPVKAAEAGYIVVVQDVRGRYASEGEFTPFVGEYEDGYDTVEWAAK
                     LPGSNGSVGTYGVSYFGMTQWQAAVMRPPSLKSMAPGITWGNHLGGVQMRGGVQELGL
                     MQYWAQTALAPDVLFRKYAGDGEKIGQKLPELIGVMDGILAGGGYDTLPLSALPNPDD
                     LVPFVQDGFKRSVTDEGWDYLNIDGKYDRVDAPTFHIGGWYDCFIGETLRQYEAMKER
                     SHEAGMPPPRLMIGPWTHGNFASTLGDLDFGVGSSGIFLNYRGDLTDAHLAWFDATLK
                     GDDSKLAETPQVQVFVMGENRWRGYEEWPVPGSREELWHLRADGGLTREEPGGESPDK
                     YDYDPTDPVPTIGGAVLMAPIHGAGPRDQRPIEERPDVLIYTSEPLPEDYTVLGNVYA
                     TLFAASSAPDTDFVARLVDVHPDGRAIGVTDGILRASARESYPAPGVIQPTKPSPIQP
                     NEVYEYAIDLWATGITFREGHRIRLEITSSSHPRWERNLNTGESAVDSADTAVAHQVI
                     FHDAEHPSCVTLTVVAG"
     gene            4724..5161
                     /locus_tag="VFJ72_06345"
     CDS             4724..5161
                     /locus_tag="VFJ72_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HET7479118.1"
                     /translation="MKPLYTAEATAHGGRRGHVESSDGKLSLDLSMPEGLGGAGGEGT
                     NPEQLFALGYSACFENALRRVARQQKIDVTDASITARVHIGRHPEGGFGLAAELIGYL
                     PNLSREEAEKLMEDAHGVCPYSRATLGNIDVQLSVKTPAEAEV"
     gene            5196..6101
                     /locus_tag="VFJ72_06350"
     CDS             5196..6101
                     /locus_tag="VFJ72_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7479119.1"
                     /translation="MTVQPQNGTEPRQFQPLAGTRLVSLAVNLPGPVAAARLRDLGAE
                     VTKVEPPAGDLLARVAPGYYDELSSGLRITILDLKQPEDRARLDGLLERADLLLTSSR
                     PASLERLGLGREKLTARFPRLSHVAIVGHPAPDDRVPGHDLTYQAGLGLLTPPDLPRT
                     LLADLAGAERAVSAALALLLSRERGGGAGYAEVSLAGSAAGFAEPLRHGLTAPGGSLG
                     GGLPEYNLYRTSDAWISVAALEPHFRRRLLEGLGLDENARYDDLARVFATRAAGEWER
                     WAENRDLPLAALREAPNTVQRQEAQ"
     gene            6098..6265
                     /locus_tag="VFJ72_06355"
     CDS             6098..6265
                     /locus_tag="VFJ72_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479120.1"
                     /translation="MTALNSASATASLGSRGRAPAQRRSEDIALTDASVTAQVNINTH
                     EPNSLFAFHAG"
     gene            6313..7491
                     /locus_tag="VFJ72_06360"
     CDS             6313..7491
                     /locus_tag="VFJ72_06360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7479121.1"
                     /translation="MSEPVILEAVRTPFGRRGGALRDVRPDSLLAHALNGLVERAGIA
                     PERIEDVVNGTVTQAGEQGANVGRLAVMLAGFPVTVPAVSINRMCGSSQQAVHFASQA
                     IAAGDMTYAIGCGVESMTRAPMFSDLGGAPDAGSGGYTRLNPALLEKQDLIHQGESAE
                     RIAERWEISRHDVDALAAESHKRASEAAREEFNREILPTPGLDADGNDVELTSDEGIR
                     GVVDAEKMAALKTVFRPEGNGVVTAGNSSQISDGASAVLVGDRERTEADGFAPKARFR
                     ARVVVGDDPTMQLTGVIPATRLALKKAGISIQDLDWIEVNEAFATVVLSWADELKPDM
                     DRVNPWGGAIAHGHPLGATGAGLMAKMLAGLEQTDGQLGLQVMCIGHGMSTATILERI
                     "
     gene            7491..8237
                     /locus_tag="VFJ72_06365"
     CDS             7491..8237
                     /locus_tag="VFJ72_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET7479122.1"
                     /translation="MQIEGRTFLVTGGGSGLGAAVVNTLAEAGANVVISDLKGEAAGE
                     NTRFVETDVTDESSVRDAVETAVRELGGLHGAVNCAGIAIAEKTLGRDGPHALDSFTK
                     VIQVNLIGTFNVIRLAAEAMTQNEPSEDGERGVIINTASVAAFDGQMGQVAYSASKGG
                     VVAMTLPIARDLARSGIRVMTIAPGIFDTAMLAGLPEEARESLGKQVPFPARLGRPDE
                     YAALARHIIENEMLNGEVIRLDGAIRMAPR"
     gene            8349..9563
                     /locus_tag="VFJ72_06370"
     CDS             8349..9563
                     /locus_tag="VFJ72_06370"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479123.1"
                     /translation="MSSGPSSRDTAHQSEDDGAGLKDSLRLLRAAAFLSTFDRFTIAP
                     MLVTIAADLRVSLAEVSAAAGVYFLLYGLMQPVWGILSDSLGRVRVIRLALIGVLVPG
                     LLSALAPNLAVLVLARAVAGGLFAAVIPAALVYIGDAVPMTARQKALSDQLAASGVAT
                     ALATALAGVAAYFGLWRLAFAVPVLASAALGLVLARLPEPEKEDGRAGPLAQLGRFFR
                     RSWALLVVALALVEGGVILGFLTYLAPSLEAAGYGAAVSGLAVGLFGVATLTWTRISP
                     RLADRFGKSALILLGGILLAAGYATGALGQNFVGVALSAFLVGGGFAFMHPTLQNWAT
                     EVLPEARATVISSFAAALFVGSGIATAAAAPLAEDGRFTLLFTLAALTGIPLGLIGSL
                     ARRRYTRRPDSR"
     gene            complement(9536..9940)
                     /locus_tag="VFJ72_06375"
     CDS             complement(9536..9940)
                     /locus_tag="VFJ72_06375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HET7479124.1"
                     /translation="MVHETTLRVRYSETDAQGIANNANYLSYFEIGRVEWLRAAGFSY
                     AGLERRGYGFVVVEAHINYKRAARFDDELTLHTKLAELKRATLRFEYELVRGDETICT
                     GYTRHGCIEIPTGRPTRIPAELDGYRESGRRV"
     gene            complement(9940..10716)
                     /locus_tag="VFJ72_06380"
     CDS             complement(9940..10716)
                     /locus_tag="VFJ72_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7479125.1"
                     /translation="MDYVKVERGDDGVAVLTIDRQEKLNALDPQVVEEIGQSLLELEA
                     DGPRAIIVTGAGEKSFVAGADIAAMSAMDPLEAKRFAEIGHAAMALLDKSPVPTIAAV
                     NGFALGGGCEIAIACDIRIAAENALFGFPEVGLGILPGMGGTQRLPRLVGPAIAKELI
                     FTGRRIKAEEAREIGLVNRVVPQGEALNAAREMAADIAANGPIAIRHSKAAANRALDV
                     DLISGLEYEADQFALLFATEDAKEGMGAFVERRKAEFKGL"
     gene            complement(10778..11176)
                     /locus_tag="VFJ72_06385"
     CDS             complement(10778..11176)
                     /locus_tag="VFJ72_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HET7479126.1"
                     /translation="MARTIRVVVAKVGLDGHDRGAKIIARALRDAGMEVIYTGLHQTP
                     EQVVEAAIQEDADAIGISILSGAHMTLIPKIMDLLKENDADDIHVFCGGTIPKQDITK
                     LKELGVGEVFTPGTPTTKAVEYVRESVAVS"
     gene            complement(11237..13099)
                     /locus_tag="VFJ72_06390"
     CDS             complement(11237..13099)
                     /locus_tag="VFJ72_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479127.1"
                     /translation="MAGHLTEEAAEALLRRFEPVIRSTRGDKFYPMDVEPYVRACSLW
                     VQRPGEGPLCVVPGGKLSLERLPQQPLDEAGAVHFLRFTDPRHIEPGSGARLGRLRRG
                     AKDLKESRESFRAGRGRLARVGYVSRFADALYSIALLARGRVPGEAAAAAAAAYRRIM
                     GGHEHYRYHGRVLRQGGWIILQYWLFYPFNDWRSGFFGANDHEADWEKVFVYLSETEA
                     GEVRPEWVAYAAHNYTGDNLRRRWDDPELEKVGEHPVIFVAAGSHASYYAPGEYLTEL
                     PVPLPRSIARGAGALRSFWQEKMGQFAGEHEGRGGSSYLSIPFVDYARGDGLTIGPGQ
                     EREWEPPRLMQDPPPAWVSGYRGLWGLYTRDPFEGEDAPAGPMYSRDKSVNRAWYDPV
                     GWAGLDKVPPPNEALEVVVAQQKSVSERREALRAEIQAKSRQLKDLGVEAAAMRDRSH
                     LDAPYQETANRIGELSGEVDGLRARLAADEAVSESLAEYANRLRAGEREPVRGHISRA
                     HRPASEDELRVSRVAEAWAAVSVSLMLISFVAIAVFEREHLISMLVVSVALFAFVEAG
                     FRGRLVNLVSSANIGLAVVAALVLVYEFFWQLAIAAVLILGLYVLWDNVRELRR"
     gene            complement(13099..13536)
                     /locus_tag="VFJ72_06395"
     CDS             complement(13099..13536)
                     /locus_tag="VFJ72_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479128.1"
                     /translation="MEADRTSYRPVRAIGLLLILQVTGLAGFGVYELAQVDWRGIDLL
                     APPRGLAEAVAFALFAPPAVLTLLSALSFLLLRRKGWLLAAISQGLSLAVCLWLYTVL
                     QPYYVYPIMAYCVLMILYLNSHDVRAMFHVRRGAEERNPGSAP"
     gene            complement(13579..14979)
                     /locus_tag="VFJ72_06400"
     CDS             complement(13579..14979)
                     /locus_tag="VFJ72_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7479129.1"
                     /translation="MDLSAGLREVLPGEGRVVTAGEAMERHGGGILTYHDPHAPDAVV
                     YPESREEIIEVLRFASENGVPVVPFGQGSSLEAHTIPVHGGISMDSGRMNNILEVRPD
                     DFIARVEPGVTHGQLNAALAEHGLFFPVDPGWDASIGGMAGTNASGTNAVRYGVMKDQ
                     VLGLEVVLGDGSVMRTGGMAMKSSAGYNLTGLFVGSEGTLGVFTEITLRLYPIPEKIL
                     AARVVFPDIEAAGRAAVAMIRSGMRIGRVELVDARTVEAVNAYKDTSYAAAPTLFLEF
                     SGSEPAVGRDVEVAQTLSDAEGCETFEFEADEAGRERLWEARHEAGLAIQALYPGRRP
                     MTTDVCVPISDLPDALRHARETIASRGLDGAILGHVGDGNYHAVFPVDPDDAEDLARA
                     EVVNEEIVEYALVRGGTCTGEHGIGSGKIGHLEREHGDSLPFMRRIKQLADPNGIMNP
                     GKIFGGAERQLPEESI"
     gene            complement(14985..>15374)
                     /locus_tag="VFJ72_06405"
     CDS             complement(14985..>15374)
                     /locus_tag="VFJ72_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7479130.1"
                     /translation="NCNDVSSRDLQFSEGGQWTRSKSIDTFCPIGPYIATRDEIEDPQ
                     DLAVRCILNGEVVQEGTTSKMIFSVAELISFLSQAMTLVPGDVITTGTPPGVGMARDP
                     QLWLKSGDEVTIEIDGLGSLTNPVTAG"
BASE COUNT         2598 a         5256 c         5059 g         2461 t
ORIGIN      
        1 tcgggccgga cggacctcct cgactcgtac tccgagacgt gcctgcgccg ggtgtggaag
       61 gcgagccggt tctcgtggtg gatgacctcc atgctgcacc gcttccacga cgacgacgaa
      121 tttcgccacc gcctgcaggt cgccgagctc gactacgtca cctcctccag ggccgcatcc
      181 acgtctctcg cggagaacta cgtgggtctg ccgatgtagc cggcagcggg ccggctactc
      241 gtcctcggcc gcctttaccg gcacgatgat gtactggagg tagccgcgcg ggttccgttc
      301 ggcctcgtct ctcagctcgc gcaggcgcca ctcggcctgc tcgcgggtcg ggtagcgggc
      361 gacctcgctc ttgccgaaga gcgtcaccgc gaccaccttg aactctcgct ctttcatccg
      421 acctccctgt tgccgcatgt tgctttacaa tgctaacatc ggcaggcacg gagacccctt
      481 gggaaaggga ggtccggcag ccaacccttt agaaggagga gtgttcgtga aaggtctggt
      541 ccaggatttc cagcttacgc tgccgtacat acttcggcgc gcggagcagc tctacgggta
      601 ccgcgagatc gtcacccgcc gccccgacaa gtccttccac cgctacactt acgcggactt
      661 cgtctcccgc gccaagaagc tcgccgtagc catgaagaag ctcggcgtgc aggacggcga
      721 ccgggtcggg acgctaggct ggaacaccta ccagcacctg gaggcgtact tcggcgttcc
      781 gtcctccggg gcggtgctcc acacgctcaa cccccggctg cacgaggatg acctggcgta
      841 catcacggat cacgccgggg accgggtgat gctcgtgaac gagaccctgg tgcccgtctt
      901 ccagaagttc cgggaccgca cgaagctgga gcacgccatc gtcgtaacgg agggcggcga
      961 ggtgccggac gggatgctct cctatgaaga cctgctcgcg gacgcggacg agtccgagtt
     1021 cgagtacccg gacttcgacg aggaccaggc cgccgcgctc tgctacacct ccgggaccac
     1081 gggccgcccg aagggcgcgc tctactcgca ccgctccacc gtgctccact ccatcatggt
     1141 ctcgatgggc aacaccttcg cgctctcgga ggccgactgc atgctgcccg tcgtgccgat
     1201 gttccacgtc aacgcctggg ggatgccgta cgcctcggtc ctcgtcgggg cgaagatcgt
     1261 aatgcccggt ccccacctgg acccgcagag cctcatcgaa gacttcgttc aggaaggcgt
     1321 cacgttcacc gccggcgtcc cgacggtctt tttggcgctg ctccaggcgc tcgataagga
     1381 gccggggaag cacgacctct cctcgatccg ggcgatggca ataggcggct cggcggcccc
     1441 gaagggcgtc ataaagaggt tccaggacga ccacggcctg aaggtcgtac acgcctgggg
     1501 gatgacggag atgagcccaa tcgggagcgt cgccaacctg ccgagtccgg tccgcgagct
     1561 atcggaggac gagcagctag attaccgggc caagcagggc tacccggtgc ccttcgtgga
     1621 gatccgggcg cgcggcgacg aggggttggt gccgtgggac gacaggagca tgggcgagct
     1681 ggaagtccgc ggcccctggg tcgcctcgac ctacttcaac acctccgagg gcgacgataa
     1741 gttcacggac gatgggtggt tcacgaccgg cgacatagta acgatagaca agtacgggtt
     1801 catcaagatc caggaccgca gcaaggacct cataaagagc ggaggagagt ggatctcctc
     1861 cgtagagctg gagaacgccc tgatggccca cccggccgtc tcgcaggccg ccgtcatcgc
     1921 catcccccac gagaagtggg acgagcgccc gctggccgcc atcgtcctga aggaaggcga
     1981 gagcgtcacg gaggaggagt tgcgagatca cctcgcgaaa gacttcgcca agttctggct
     2041 cccggacgcc tacgagttcg tcgaggagat cccgatgacc gctaccggca agttccagaa
     2101 gctgaagctg cgcgagcagt tcgagggcta cacgacgacc ggcagctaat agccggcgtg
     2161 aaggtgatcg gagccggctt cggccggacg ggaactacct cgctgaaagc ggccctggag
     2221 actttaggct tcggtccctg ctaccacatg acggaggtct tcgcgcaccc gcgccacgcg
     2281 agcacctggg atgcggcgtg gcggggagag ccggtggact gggacgcctt tctcgcaggc
     2341 tacgaggcga ccgtggactg gccggcctgc acgttctaca gagaactcat ggaggagtac
     2401 ccggaggcga aggtgctcct gagcgtgcgc gaccccgaac ggtggtacga gagcgtgcga
     2461 agcaccatct acgagttgag cgagtccttc atccgctctc cggtcttccg cgcgatcttc
     2521 ggcctcacca gcttcttcgc ttttggcggc tttttacgct ccagttgggg agacatggtg
     2581 aacgaaataa tcttacaggg cacattcgac gggcgactgg aagacaaagc ctacgcgatg
     2641 tgggtcttcg agcggcacaa cgaggaggta aaacggagcg taccaccgga ccggctgctc
     2701 gtctacgagg taaaggagag ctgggggccg ctctgcgagt ttctcggcgt cccggagccg
     2761 gacgcgccgt tcccgcgcct caacgacgga gcgcagatgc agcgagctat aagaacgatc
     2821 cgggggctct cctttgcatt gcccgttaca gtaatactgg tgccctgtgc cgcggccctc
     2881 gcactgctcc gcaggcgcgc cagaccctga caagccactt accgaaccca gaacggaggc
     2941 tccttgaacg agacacggga gataatagtc cagaaggacg ttcccgccga gatgcgcgat
     3001 ggaaccaacc tgatgtctaa cgtctaccgg ccgaacgacg agggcgacta cccggtgctt
     3061 ctcactcgtc tgccctacgg caaagacctg ccgctcggca cctcgtacct ggacccggtg
     3121 aaggcggcgg aggcgggcta catagtcgtc gtgcaggacg tgcggggccg gtacgcttcg
     3181 gagggcgagt tcaccccgtt cgtgggggag tacgaggacg gttacgacac cgtcgagtgg
     3241 gcggcgaagc tgcccggttc caacggcagc gtcgggacgt acggcgtctc ctacttcggc
     3301 atgacccagt ggcaggccgc cgtaatgcgc ccgccgtccc tgaagagcat ggcccccggc
     3361 atcacgtggg gcaaccacct gggcggcgtc cagatgcgcg gtggcgtaca ggagctgggc
     3421 ctgatgcagt actgggcgca gaccgcgctc gcgccggacg ttctcttccg caagtatgct
     3481 ggagacggcg aaaagatcgg ccagaagctg ccggagttga tcggggtgat ggacggtatc
     3541 ctggccggcg gcggatacga taccctcccc ctctccgccc tcccgaaccc cgacgacctc
     3601 gtcccgttcg tgcaggacgg cttcaagcgc agtgtgaccg acgagggctg ggactacctc
     3661 aatatagacg ggaagtacga cagagtagac gccccaacct tccacatcgg cggctggtac
     3721 gattgcttta tcggggagac gctgcggcag tatgaggcga tgaaagagcg ctcgcacgag
     3781 gccgggatgc ccccgccgcg gttgatgatc gggccgtgga cgcacggcaa cttcgccagc
     3841 acactcggcg acctggactt cggcgtcgga agctccggca tcttcctaaa ctatcgggga
     3901 gacctgaccg acgctcacct cgcctggttc gacgcaacgc tcaaaggtga cgactcgaag
     3961 ctcgcggaga cgccgcaggt acaggtcttc gtaatgggcg agaaccgctg gcgcggctac
     4021 gaggagtggc cggtccccgg ctcgcgcgag gagctgtggc acctgcgcgc ggacggcggg
     4081 ctcacccgag aagagcccgg cggcgagtct ccagacaagt atgactacga ccccacggac
     4141 cccgtcccga ccatcggcgg cgcggtgctc atggccccca tccacggcgc cggtccgcgc
     4201 gaccagcgac ccatcgagga gcgtcccgac gtgctcatct acaccagcga gccgctaccg
     4261 gaggactaca ccgtcctcgg gaacgtgtac gcaacgctct tcgcggcgtc ctccgcgccg
     4321 gacacggact tcgtggcgcg gctcgtggac gtccacccgg acgggcgagc catcggggtg
     4381 acggatggga tcctccgggc aagcgcccgg gagagttatc cggcgccggg tgtgatccag
     4441 ccgacgaagc cgagtccgat acagccgaac gaggtttacg agtacgcgat agacctgtgg
     4501 gctaccggca tcaccttcag agaggggcat cggatacggc tggagatcac ctcaagctcc
     4561 cacccccgct gggagcgcaa cctgaacacc ggcgagagcg ccgtggattc cgcggacacc
     4621 gctgtcgcgc atcaggtcat cttccacgac gcggaacacc cgagttgcgt cacgctgacc
     4681 gtggtagcag gatagaagga caccaaggac aggaggagga aggatgaagc cactgtacac
     4741 cgcggaagcg acggcgcacg gaggccgccg cggacacgtc gagtcctcgg acggcaagct
     4801 gagcctggac ctctcgatgc cggaggggct cggcggagcc ggcggagaag gcaccaaccc
     4861 cgagcaactc ttcgccctcg ggtactcggc gtgcttcgag aacgccctgc gccgcgtcgc
     4921 tcgccagcag aagatagacg tgaccgacgc ctccatcacg gcgcgggttc acatcggccg
     4981 gcaccccgag ggcggcttcg ggctggcggc agagttgatc gggtaccttc caaacctgtc
     5041 gagggaagag gccgagaagt tgatggagga cgcccacggg gtctgcccct actcgcgcgc
     5101 cacgctgggg aacatagacg tccagctctc cgtaaagacg ccggcggagg ccgaggtctg
     5161 acattgcgaa ccgtcccccc agcgaggtaa gcgccgtgac cgtgcagccg cagaacggga
     5221 cagagccccg tcagttccag cccctggccg ggacgcggct cgtgtcgctg gccgtgaacc
     5281 ttccgggacc ggtcgccgca gccaggctcc gcgacctcgg cgccgaggtg acgaaggtcg
     5341 agccgccggc cggagacctg ctagcccgcg tcgcccccgg atactacgat gagctatcca
     5401 gcggcctgcg catcaccatc ctcgacctca agcagccaga ggatcgcgcc cggctcgacg
     5461 gtctcctcga acgcgccgac ctcctgctca cctccagccg tcccgcctct ttggagagac
     5521 tggggctcgg ccgggagaag ctgacggccc ggttcccccg gctctcgcac gtcgccatcg
     5581 tcggccaccc ggctcccgac gacagagtgc cgggccacga cctcacttac caggccggac
     5641 tcgggctcct cacgcccccg gacctgccgc gcaccctgct cgcggacctg gcaggcgccg
     5701 aaagggcggt gagcgcggcg ctggcgctgc ttctatctcg ggagcgggga ggcggcgcgg
     5761 gttacgcgga ggtatcgctg gcggggtcgg cggcgggctt cgccgagccg ctgcgccacg
     5821 gtctgacggc tccgggcggg agcctcggcg gagggttgcc agagtacaac ctgtaccgga
     5881 ccagcgacgc ctggatctcc gtggccgccc tcgaaccgca cttccgacgg cgtctactcg
     5941 aagggctcgg cctcgacgag aacgccagat acgacgacct ggcgcgcgtc ttcgccaccc
     6001 gcgccgccgg agaatgggag cggtgggcgg agaatcgcga cctgccgctg gcggcgctgc
     6061 gcgaagcgcc taacacagtc cagagacagg aggcccaatg acggcactga acagcgcgag
     6121 cgccacggcc tccctgggca gtcgaggacg cgctccggcg cagcgtcggt cagaagatat
     6181 agccctcacc gacgcctccg ttaccgcaca ggtgaacata aacacgcacg aacccaactc
     6241 cctattcgcg ttccacgcgg ggtaacatag acgtcgagct ttctgtaaaa ccttgagcga
     6301 gaggaccaag agatgagcga gcccgtcata ctggaagccg tccgcacacc attcggcagg
     6361 cgcggcggcg cgctccggga cgtgcgtccc gactcgctgc tcgcccacgc gctaaacggt
     6421 ctcgtcgagc gggccgggat cgccccggag cggatagaag acgtcgtcaa cggcaccgtc
     6481 acgcaggccg gggagcaggg cgcgaacgtc ggcaggctcg cggtgatgct cgcagggttt
     6541 ccggttaccg taccggcagt ctcgatcaac cgcatgtgtg gatccagcca gcaggccgtc
     6601 cactttgctt cccaggcaat cgccgccgga gacatgacct acgccatcgg gtgcggagtc
     6661 gagagcatga cgcgcgcgcc gatgttctcc gacctcgggg gggcgccgga tgcgggatcc
     6721 ggcgggtaca cgcggctcaa cccggccctg ctggagaagc aagacttgat ccaccagggc
     6781 gagagcgccg agcgcatcgc ggagcgatgg gagataagcc gccacgacgt ggacgccctc
     6841 gccgccgaga gccacaagag ggcgagcgag gccgcccgtg aggagttcaa ccgggagata
     6901 ctccccaccc ccggcctcga cgccgacggc aacgacgtgg agctgacgag cgacgaaggc
     6961 atccggggcg tcgtggacgc ggagaaaatg gcggcgctaa agaccgtctt ccggccggaa
     7021 gggaacggcg tcgttacggc aggcaactcg tcccagatct ccgacggcgc ctccgccgtc
     7081 ctcgtcggcg accgcgaacg gaccgaggcg gacggcttcg caccgaaggc gcgtttccgg
     7141 gcgcgggtcg tggtcggcga cgacccgacg atgcagctta caggcgtcat cccggcgacg
     7201 cggctagcgc tgaagaaggc gggcatctcc atccaggacc tggactggat cgaggtgaac
     7261 gaagcattcg ccaccgtcgt cctctcctgg gccgacgagc tgaagccgga catggacagg
     7321 gtaaatccgt ggggtggagc gatagcgcac ggacatccgc tcggggcgac cggcgcgggc
     7381 ctgatggcga agatgctcgc cggtctcgaa cagacggacg ggcagctcgg gcttcaggtc
     7441 atgtgcatcg gccacggcat gtccaccgcg accatactgg agcgcatcta atgcagatag
     7501 agggacgcac gtttctcgtg accggcggcg gctcggggct cggcgcggcc gtcgtaaaca
     7561 cgctcgccga ggccggggcg aacgtcgtga tctcggacct caagggtgaa gccgccggcg
     7621 agaatacccg cttcgtggag accgacgtta cggacgaatc gagcgtccgg gacgccgtcg
     7681 agactgcggt gcgggagctc ggcgggctgc acggcgcggt caactgcgcc ggcatcgcca
     7741 tagcggagaa gaccctcggc agggatggtc cgcacgcgct cgactcgttc accaaagtca
     7801 tccaggtgaa ccttatcggg accttcaacg tcatccggct cgcggcggag gcgatgacgc
     7861 agaacgagcc ctcggaggac ggagagcgcg gcgttatcat caacaccgcc tcggtcgcgg
     7921 cattcgacgg gcagatgggc caggtcgcct actcggcctc taaaggcggc gtcgtcgcca
     7981 tgacgctccc aatcgccagg gacctcgcgc ggagcggcat ccgggtgatg accatcgccc
     8041 ccggcatctt cgacaccgcc atgctcgcgg gactacccga agaggctcgt gagtctctcg
     8101 gcaagcaggt cccgttcccc gcgcggctcg gccgtcccga cgagtacgcg gcgctcgcgc
     8161 ggcatatcat cgagaacgag atgctcaacg gcgaggtcat ccgtctggac ggcgcgatcc
     8221 ggatggcccc gcgttaaccg ttccactctg gagaggcacc atgtctccgg cgctctctca
     8281 aggtccggaa ggcgaagaga acctgaacga gcttgtctcg agacacggac ttccgtaatg
     8341 ttgctcccgt gagttccggc ccttcatcca gagatacagc ccatcaaagc gaagatgacg
     8401 gcgccggcct gaaagacagc ctgcggctct tgcgggcggc ggcgttcctc agcacgttcg
     8461 accggttcac gatagccccg atgctcgtta ccatcgcggc tgacctccga gtctcgctgg
     8521 cggaggtgtc ggcggctgcg ggcgtctact tcctgctcta cggtctgatg cagccggtgt
     8581 ggggcattct ttcggacagc ctcggtaggg tgcgcgtgat ccgcctcgct ctcatcggag
     8641 tcctcgtacc gggattgctc tcggccctgg cacccaacct ggcggttctg gtgttggcgc
     8701 gagccgtggc gggcgggcta ttcgcggcgg tcataccggc cgctctggtc tacataggcg
     8761 acgccgtgcc gatgacggcg cggcagaagg cactctcgga ccagctcgcc gcgagcggcg
     8821 tggcgacggc gctggctacc gcgctggcgg gcgtcgccgc ctacttcggg ctctggcggc
     8881 tcgccttcgc ggtcccggtc ctggcctccg ccgcgctggg gctcgtgctc gcgcggcttc
     8941 cagagccgga gaaggaggac ggacgcgccg ggccgctcgc gcagttagga cgcttcttcc
     9001 gccggtcgtg ggcgctcctg gtagtggcgc tcgctctggt ggagggaggc gtgatcctgg
     9061 gcttcctcac ctacctggcc ccttctctcg aagccgcggg ctacggcgcg gcagtctcgg
     9121 gactcgccgt cgggttgttc ggggtggcga cgctgacctg gacccggatc tccccgcgcc
     9181 tcgccgacag attcgggaag tccgccctaa tcctgctcgg cggcatcctc ctcgcggcgg
     9241 gctacgccac cggcgccctg ggccagaact tcgtcggcgt cgcgctctcg gccttcctgg
     9301 tcggcggcgg cttcgccttc atgcacccca ccctccagaa ctgggccacc gaggtcttgc
     9361 cggaagcccg tgcgaccgtc atctcctcct tcgccgccgc cctcttcgtc ggcagcggca
     9421 tagcaaccgc cgcggcggcg ccgctcgccg aggacggccg cttcaccctc ctcttcaccc
     9481 tcgccgccct cacggggata ccgctcggcc tgatcgggag cctcgcccgc cgccgttaca
     9541 cgcgccggcc ggactcccgg tagccatcca gctcagccgg tatgcgagtc ggcctgcccg
     9601 tcgggatctc gatgcacccg tgccgcgtgt agccggtgca gatagtctca tccccgcgca
     9661 ccaactcgta ctcgaacctt agcgtggccc gcttcagctc cgcgagcttg gtatggagcg
     9721 tcagctcgtc atcgaagcgg gcggcgcgtt tgtagttgat gtgcgcctcc acgacgacga
     9781 agccgtagcc gcgacgctcc aggccggcgt aggagaaccc ggcggcgcgc agccactcga
     9841 cgcgcccgat ctcgaagtac gagaggtagt tggcgttatt ggctatgccc tgcgcgtcgg
     9901 tctcggagta ccggacgcgc agggtagtct cgtggactat tacaggccct tgaactcggc
     9961 cttgcggcgc tcgacgaagg cgcccatgcc ctccttggcg tcctcggtgg cgaagaggag
    10021 ggcgaactgg tcggcctcgt actccaagcc gctgatgagg tccacgtcga gggcgcggtt
    10081 ggcggcggcc ttggagtggc ggatggcgat gggaccgttc gcggcgatat cggcggccat
    10141 ctcgcgcgcg gcgttcaggg cctcaccctg cggcacgacg cggttgacca gcccgatctc
    10201 acgcgcctcc tccgccttga tgcgccgtcc cgtgaagatg agttccttgg cgatggcggg
    10261 tccgacgaga cgcggcaagc gctgcgtccc gcccatgccg ggcaggatgc cgagcccgac
    10321 ctccgggaag ccgaaaaggg cgttctcggc ggcgatccgg atatcgcagg caatggcgat
    10381 ctcgcacccc ccgccgaggg cgaagccgtt tacggcggct atcgtgggga cggggctctt
    10441 gtccaggagg gccatcgcgg cgtgcccgat ttccgcgaag cgcttcgcct ccagggggtc
    10501 catcgcgctc atcgcggcta tgtccgcacc cgcgacgaag gacttctccc ccgcgccggt
    10561 cacgatgatc gcgcgcggcc cgtccgcctc cagctccagc agcgactgcc cgatctcctc
    10621 gacaacctgc gggtcaaggg cgttcagctt ctcctgccgg tctatcgtca ggacggcgac
    10681 gccgtcatcc ccgcgctcca ccttaacgta atccaaagcg tctcctcgcg tagaacctct
    10741 aaagcacttc gttcaagaat tccggccggc aggctgacta gctgacggct accgattccc
    10801 ggacgtactc tacagctttc gtggtgggag ttccgggagt gaagacctcg ccgaccccga
    10861 gttccttcag cttcgtgatg tcctgcttcg ggatcgtgcc gccgcagaag acgtggatgt
    10921 cgtcggcgtc gttctccttc aggaggtcca tgatcttcgg tatcagggtc atgtgcgcgc
    10981 cggagaggat ggagatgccg atggcgtcgg cgtcctcctg tatggcggcc tcgacgacct
    11041 gttcaggggt ctggtgcagg ccggtgtaga tgacctccat cccggcgtcg cgaagggcgc
    11101 gggcgatgat cttggccccc cggtcgtgac cgtcgagccc aacttttgca acgactaccc
    11161 gtatcgtgcg cgccatagtg cttctatttt aaccatatcc tggccccgcg cgacaccgcc
    11221 tcccagcgag cgccgtttat ctgcgcagct ccctcacgtt gtcccacagg acgtagaggc
    11281 ccaggatcag gaccgccgcg atagcgagct gccagaagaa ctcgtagacg agcaccagcg
    11341 cggcgacgac cgcgagcccg atgttggcgc tgctgaccag gttcacgagc cgcccccgga
    11401 agcccgcctc gacgaacgcg aacagggcga cgctgacgac gagcatggag atcagatgtt
    11461 cccgctcgaa cacggctatc gccacgaagc ttattagcat caggctgacg ctgacagcgg
    11521 cccacgcctc ggccacgcgg ctgacgcgaa gctcgtcctc cgacgccggc cggtgagcgc
    11581 gggagatgtg accgcgtact ggctcccgct ctcccgccct caagcggttg gcgtattctg
    11641 caagcgactc cgaaacagcc tcgtcggcgg cgagccgcgc ccgcagcccg tccacctccc
    11701 cggagagctc cccgatgcga ttagccgtct cctggtacgg cgcgtccagg tgagacctgt
    11761 cgcgcatggc agcggcctcc acgcccaggt ccttgagctg ccggcttttg gcctggatct
    11821 cggcgcgcag cgcctcgcgg cgctcggaga cgcttttctg ctgggcgacg accacctcca
    11881 gcgcctcgtt cggaggcggc accttgtcga gcccggccca cccgaccggg tcgtaccagg
    11941 ctcggttgac gctcttgtcg cggctgtaca tcggaccagc cggggcgtct tcaccctcga
    12001 aggggtcgcg ggtgtagagg ccccagagcc cccggtaccc ggagacccag gccggaggcg
    12061 ggtcctgcat gagcctcgga ggttcccact ccctctcctg ccccggcccg atagtgaggc
    12121 catccccgcg agcgtagtcc acgaagggga tgctcaggta gctggaccct cccctcccct
    12181 cgtgctcccc ggcgaactgg cccatcttct cctgccagaa cgaccggagg gccccggcgc
    12241 ccctggcgat cgagcgtggc aggggaacgg gcagctcggt caggtactcg cccggagcgt
    12301 agtagctggc gtgagagccg gcggcgacga agatcaccgg gtgctcccca accttctcaa
    12361 gctccgggtc gtcccatcgg cgcctgaggt tgtccccggt gtagttgtgg gcggcgtagg
    12421 ctacccactc cgggcgcacc tcacccgcct ccgtctcgga gaggtagacg aagaccttct
    12481 cccagtcggc ctcgtgatcg ttggcgccga agaagccgct cctccagtcg ttgaacgggt
    12541 agaagagcca gtactgcaag atgatccacc cgccctggcg cagcacccgc ccgtgatacc
    12601 tgtagtgctc gtgtccgccc atgatgcgcc ggtaagccgc cgcggccgct gcggcggctt
    12661 ctccggggac ccgaccgcgg gccagcagcg cgatggagta gagggcatcc gcgaagcgtg
    12721 agacgtatcc gacgcgggcc agccggcccc gcccggccct gaaagactcc cgggactcct
    12781 tgaggtcctt cgcgcctctg cgtaaccgtc ccagacgcgc gcccgaacca ggctcgatgt
    12841 gcctggggtc ggtaaacctg aggaagtgga ccgccccggc ctcatctagg ggctgctggg
    12901 ggagccgctc caggctcagc ttcccgcccg gcacgacgca gaggggcccc tcgccggggc
    12961 gctggaccca caggctgcac gcccgcacgt agggctcgac gtccatcggg tagaacttgt
    13021 cccctctcgt ggagcggatg acaggctcga agcgccggag cagggcctcg gccgcctcct
    13081 ccgtgagatg cccggccatc acggcgcgct ccctgggttc cgctcttccg cgccgcgcct
    13141 gacatgaaac atcgcccgaa cgtcgtggga gttgaggtac aggatcatca ggacgcagta
    13201 agccatgatc gggtagacgt agtacggctg gagcacggta tacagccaca ggcagaccgc
    13261 caggctcagg ccctgggaga tcgccgccag aagccatcct ttgcggcgca acagcaggaa
    13321 actcaacgcc gagagcagcg tcaggaccgc gggcggagcg aagagggcga acgccaccgc
    13381 ttccgcaagc cccctgggag gcgccagcag gtctatcccc cgccaatcca cctgcgcgag
    13441 ctcgtacacg ccgaaacccg ccagcccggt gacctgcaaa ataagcagca ggccgatggc
    13501 tcttacgggc cggtaggatg tcctgtccgc ctccacatcc ctattctaca agccgtccgc
    13561 ggctcttccc cacctcggtc agatagactc ctcgggcagt tgccgttccg cgcctccgaa
    13621 tatctttccg gggttcatga tgccgttcgg atctgcgagc tgctttatgc ggcgcatgaa
    13681 cggcagcgag tcgccgtgct ccctctccag gtggccgatc ttgccggagc cgatgccgtg
    13741 ctcgccggtg caggtcccgc cccggacgag ggcgtactcc acgatctcct cgttcacgac
    13801 ctcggctctc gccaggtcct ccgcgtcgtc cgggtccacg gggaagacgg cgtggtagtt
    13861 gccgtcaccg acgtggccga ggattgcgcc gtcgaggcca cgcgaggcta tcgtctcacg
    13921 agcgtggcgc agggcgtccg ggagatcgga gatcgggacg cacacgtccg tggtcatggg
    13981 ccgcctgccg gggtagaggg cctggatggc aagccccgcc tcgtggcgag cctcccacag
    14041 cctctctcgt cccgcctcgt ccgcctcgaa ctcgaaggtc tcgcagccct ccgcgtccga
    14101 gagggtttgc gccacctcca cgtcgcgccc gacggcgggc tccgagccgc tgaactccag
    14161 gaagagcgtc ggagcggccg cgtaggaggt gtccttgtag gcgttcactg cctcgacggt
    14221 ccgggcgtcc acgagctcca cgcgcccgat cctcatgccg gaccggatca tggctaccgc
    14281 cgccctcccc gcggcctcga tgtccgggaa gactacgcgc gcggcgagta tcttctccgg
    14341 tatcgggtac agccgcagcg ttatctccgt gaagacgccc agcgtgccct ccgaacccac
    14401 gaagagcccg gtcaggttgt agccggcgga cgacttcatc gccatcccgc cggtccgcat
    14461 cacgctcccg tcgcccagca ccacttcaag gccgaggact tgatctttca tcaccccgta
    14521 gcgcaccgcg ttcgtgccgc tggcgttggt cccggccatc ccgccgatgg aggcgtccca
    14581 gccggggtcc accgggaaga acagcccgtg ctccgcgagc gccgcgttga gctgaccgtg
    14641 cgtgacgccc ggctcgaccc tggcgatgaa gtcgtccggc cggacctcca ggatgttatt
    14701 catacggcca gagtccatgc tgatgccgcc atgcaccgga atggtgtggg cctccaggct
    14761 gctcccctgc ccgaacggca cgaccggcac gccgttctcg gaggcgaagc gcaggacctc
    14821 gatgatctct tcccggctct ccgggtagac gacggcgtcc ggagcgtgcg ggtcgtggta
    14881 ggtgaggatg ccgcccccgt gacgctccat cgcctctccg gcggtgacga cgcgcccctc
    14941 gcccggcaag acctcgcgca gtccggcaga caggtccatc gcgcctaccc cgcggtgacc
    15001 gggttggtca gggagccgag accgtcaatc tctatcgtca cctcatcgcc ggacttcagc
    15061 cagagctgcg ggtcgcgggc catcccgacg cccggaggcg tgccggtcgt gatgacgtcg
    15121 ccggggacga gggtcattgc ctgcgagagg aacgagatca gctcagcgac ggagaagatc
    15181 atcttcgagg tcgtcccctc ctgcaccacc tcgccgttca ggatgcaccg caccgcgagg
    15241 tcctgagggt cttctatctc atcgcgggta gcgatgtacg ggcctatcgg gcaaaaggtg
    15301 tctatggact tgcttcgcgt ccactgcccg ccctcggaga actgaaggtc cctggacgag
    15361 acgtcgttgc agtt
//
LOCUS       DASSMB010000098         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_20888, whole genome shotgun sequence.
ACCESSION   DASSMB010000098 DASSMB010000000
VERSION     DASSMB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20888"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFJ72_06410"
     CDS             <1..270
                     /locus_tag="VFJ72_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET7479131.1"
                     /translation="ARIQGTAADIIKVAMVDLQPRLRSLGTDMLMQVHDELVFDADER
                     RVPVVAALVADRMVGAYDLDPPLEVEVKVGERWGQVRPYLKDRVE"
     gene            413..1696
                     /gene="rpsA"
                     /locus_tag="VFJ72_06415"
     CDS             413..1696
                     /gene="rpsA"
                     /locus_tag="VFJ72_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7479132.1"
                     /translation="MTETTKNATRELTMRDFFREENGEVVPVDESVLIDFKDGDIVEG
                     EVVRIDKDEVLVDIGYKSEGLIPSNELSIRKGADPTEIVELGQHIEALVLQKEDADGR
                     LILSAKRAAFEKAWNRIEEAYNEQRVVEGPVIEVVKGGLIIDIGLRGFLPASLVDIRR
                     VRNLDSFLGQKLECKVIELNRSRNNVVLSRRAVLEEERKEEREKILTSLEEGQIVTGT
                     VSNLVDFGAFVDLEGIDGLIHISELSWNHVDHPSEVVEVGEEVEVKVLEVDRDRERIS
                     LGLKQTRKDPWQEIVEQVEIGGQIKGRVTKLVSFGAFVEVAEGVEGLIHISELADHHV
                     ETPDEIVRSGDEVDARIIDVDAKRRRLSLSLRPKKEDREEREGGDDRPRREDRPRRDD
                     RPRRDEPREQRTATSGSGLRTGAFDKLSDLDLGRE"
     gene            1735..2352
                     /gene="coaE"
                     /locus_tag="VFJ72_06420"
     CDS             1735..2352
                     /gene="coaE"
                     /locus_tag="VFJ72_06420"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET7479133.1"
                     /translation="MTIGVTGPFASGKSTLVQLLGALGAETVSADGVVHDLLSGDAET
                     IRRVVERFGEEVRGESGIDRQALGREVFGDSAALKDLEGILHPLVRREVSRRAAASDA
                     DVFVAEIPLLFEVGQEGAYDLTVAVVTSTERRKEWSEERGVDEPRRRAIEARQFTGEE
                     KALRADVVVQNDGDLERLREQAGELWEMALRERRSGGSLQEQEES"
     gene            2396..2929
                     /locus_tag="VFJ72_06425"
     CDS             2396..2929
                     /locus_tag="VFJ72_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HET7479134.1"
                     /translation="MLLLLILLAALPLLLKTPDTVQRATHPLRYEGLIRAQSERYDLE
                     PTFVAGVVYTESRFKPDVQSHQGAYGLMQILPQTADFISERGGIKGDYRDPRTNLKMG
                     IWYLDYLDERYLGDERLMLAAYNSGEGRVDEWISDKGFDVGKDIPFQETHEYVDNVLQ
                     AQQTYEDLYGRNLDRRP"
     gene            2982..>4708
                     /gene="uvrB"
                     /locus_tag="VFJ72_06430"
     CDS             2982..>4708
                     /gene="uvrB"
                     /locus_tag="VFJ72_06430"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HET7479135.1"
                     /translation="MEQLATGLDSGLKAQTLLGVTGSGKTHTMARVIEKVGRPSLVIA
                     HNKTLAAQLASEFAEFFPNNAVEYFVSYYDYYQPEAYVPRTDTFIEKDAQINEDIDRL
                     RHSATSSLFTRRDVIVVASVSAIYGLGSPEEYRSKMVLLEQGGFYDLDDILRNLVKIQ
                     YTRNDFQLARGSFRVRGDVLEVHPAYQDSIYRVSFFGDEVESMVEADPLTGEVLREMD
                     TLTVYPATHFVTNEDRMAVAIKNIEAEMEQRTAELETEGKVLEAYRLRQRTQYDLEMM
                     RELGYCSGIENYSRHMDGRPAGSTPYTLLDYFPDDYVTFVDESHITLPQIRGMYNGDR
                     SRKTTLVEFGFRLPSALDNRPLMWEEFLLKTDQMVFVSATPGPYELENSGQIVEQIIR
                     PTGLIDPEVEVRPTKNQIDDLLNEVAKRVEHGERVLITTLTIKMSEDLTDYLLEHNVK
                     ARYMHSNIETLDRIQIIRGLRTGEFDVLVGINLLREGLDLPEVTLVAILDADKEGFLR
                     GERALIQTIGRAARNVNGRVIMYADRETDSMRLAIGETNRRREIQTAHNERNNIVPRT
                     ISKGVSDIL"
BASE COUNT          952 a         1398 c         1599 g          759 t
ORIGIN      
        1 gcccgcatcc agggtacggc ggcggatatt atcaaggtgg cgatggtgga cctgcagccg
       61 cggctgcggt ctctcggcac ggatatgctg atgcaggtac atgacgagct ggtcttcgac
      121 gcggatgagc gccgggtgcc ggtggttgcg gcgctcgtcg ccgacaggat ggtcggggcc
      181 tacgacctgg acccgccgct ggaggtcgag gtcaaggtgg gggagcgctg gggccaggtg
      241 cgcccttatc tcaaagatag ggtagaatag ttaaggttaa gcaacaaacc ggtaacgcac
      301 cctttatggt gcctgtgtct cggaagccag attctcgggt gctgtcgcca taaaacttgt
      361 ataatcgcgc cggcacgcca gcaagctagg aaaaaaggaa agctaccagt acatgacgga
      421 aaccaccaaa aacgcgaccc gagagttgac catgagggac ttcttccggg aggagaacgg
      481 cgaggtcgtc cccgtggacg agagcgtcct catagacttc aaggacggcg acatcgtcga
      541 gggcgaggtc gtgcgcatag acaaggacga agtcctcgtg gacatcgggt acaagtcgga
      601 ggggctcatc ccttcgaacg aactctcgat ccgcaagggc gcggacccga cggagatcgt
      661 cgagctcggt cagcacatcg aggctctcgt gctgcagaag gaggacgccg acgggaggct
      721 catcctctcg gccaagcggg cagccttcga gaaggcctgg aaccgcatcg aggaagccta
      781 taacgagcag cgcgtcgttg agggtccggt catcgaggtg gtgaagggag gtctgatcat
      841 cgacatcggc ctccgcggat tcctgccggc cagcctggtt gacatccggc gcgtgaggaa
      901 cctggactcc ttcctcgggc agaagctgga gtgcaaggtc atcgagctca accgcagccg
      961 caacaacgtg gtgctctccc gccgggccgt tctggaggag gagcgcaagg aggagcgcga
     1021 gaagatcctg acctctctcg aagagggcca gatcgtcacg ggcaccgtct ccaacctcgt
     1081 ggacttcggc gcgttcgttg acctggaggg tatagacggc ctgatccaca tctccgagct
     1141 atcgtggaac catgtcgacc atcccagcga ggtcgtcgag gtcggtgaag aggtcgaggt
     1201 caaggtgttg gaggtcgacc gggaccgtga gaggatctcg ctgggtctca agcagacccg
     1261 gaaggacccg tggcaggaga tcgtcgagca ggtcgagatc ggtgggcaga tcaagggccg
     1321 tgtcaccaag ctggtctctt tcggcgcgtt cgtcgaggtc gccgagggtg tcgagggcct
     1381 gatccacatc tccgagcttg ccgaccatca tgtggagacg ccggatgaga tcgtgcgctc
     1441 cggcgacgag gtggacgccc gcataatcga cgtggacgcg aaacgccgcc ggctctcgct
     1501 ctccctgcgt ccgaagaagg aggaccggga ggagcgcgag ggtggagacg accgtccgcg
     1561 ccgcgaggat cggccccgcc gggacgaccg cccgcgccgc gacgagccgc gtgagcagcg
     1621 caccgccacc agcggcagcg gcctgcgcac cggcgcgttc gataagctct ccgacctgga
     1681 cctgggccgg gaatagcacc cgcttcggaa cagggaaggt cctcgaaggg gccggtgacc
     1741 atcggcgtca ccggcccctt tgcctccggc aagagtaccc tcgtccagct actcggcgcg
     1801 cttggagcgg agacggtatc ggcggacggg gtcgtccacg acttgctctc gggtgacgcg
     1861 gagactatca ggcgcgtcgt cgaacgcttt ggagaggagg tccggggcga gagcggtata
     1921 gaccggcagg ctctcggccg ggaggtcttc ggcgactcgg cggcgctgaa ggacctggag
     1981 ggtatactgc accccctggt gaggcgagaa gtttcgcgtc gcgccgccgc ctcggatgcc
     2041 gatgtcttcg tcgccgagat accgttgctc ttcgaggtgg ggcaggaggg cgcttacgac
     2101 ctgacggtcg cggtggtaac gtccacggag cgccggaagg agtggtcgga ggagcggggg
     2161 gtggatgagc cgcggcgccg ggcgatagag gcgcgccagt tcacgggcga ggagaaggca
     2221 ctccgggcgg atgtcgtggt acaaaacgat ggagacctgg agagactcag ggagcaggct
     2281 ggagagttgt gggagatggc tctccgggag aggagatccg gcggtagctt acaggagcaa
     2341 gaagagagct aggtacggtc ggtcccggac ccgccggcgc cggctggctg tcggggtgct
     2401 actgttgctc atcctgctgg ccgcgctgcc gctgctcctc aagacgcccg acaccgtgca
     2461 gcgggcaacg cacccgctgc ggtacgaggg tttgatccgt gcccagagcg agaggtacga
     2521 cctggaaccg acgttcgtgg caggggtggt ctacaccgag agccggttca agccggacgt
     2581 ccagtcccat cagggggcgt atgggctcat gcagatactc cctcagactg cggacttcat
     2641 ctccgagcgc ggcgggataa agggcgacta ccgcgacccg cggacgaacc tcaagatggg
     2701 catctggtat ctggattatc tcgacgagcg gtatctgggt gatgagaggc tcatgctcgc
     2761 cgcgtataat tccggggagg gccgggtgga cgagtggatc tccgacaagg gcttcgacgt
     2821 cgggaaggac ataccgttcc aggagaccca cgagtatgtg gacaacgtcc tccaggcgca
     2881 gcagacctat gaagatctat acgggaggaa cctagatcga cgtccgtaac acattcagcg
     2941 taaatagcga ataccagccg accggcgacc agccccaggc catagagcag cttgctacgg
     3001 ggctagattc cggcctgaag gcccagaccc tgctcggcgt taccggctcg ggcaagacgc
     3061 acacgatggc ccgcgtgatc gagaaggtcg ggcggccgtc gctcgtgatc gcgcacaaca
     3121 agacactcgc cgcgcagctc gccagcgagt tcgccgagtt cttcccgaac aacgccgtcg
     3181 agtacttcgt cagctactat gactactacc agcccgaggc atatgtcccg cgcacggaca
     3241 ccttcatcga aaaagacgcc cagatcaacg aggacataga ccgtctgcgg cactccgcca
     3301 cgagcagcct tttcacgcgc cgggacgtga tcgtggtggc gagcgtctcc gcgatctacg
     3361 gtctgggaag cccggaggag taccgctcga agatggtgct tctggagcag ggcggcttct
     3421 acgatttgga cgacatcctg aggaacctcg tcaagatcca gtacaccagg aacgacttcc
     3481 agcttgcgcg cggctctttc cgggtgcgcg gcgacgtgct ggaggtccac ccggcgtacc
     3541 aggactccat ctaccgcgtc tcgttcttcg gcgatgaggt ggagagcatg gtcgaggccg
     3601 accctctgac cggcgaagtc ctgcgtgaga tggacactct caccgtctac ccggcgaccc
     3661 acttcgtcac caacgaggac cggatggccg tcgcgataaa gaacatcgag gcggagatgg
     3721 agcagcgcac ggcggagctg gagacggagg gcaaggtcct cgaagcgtac cggctccggc
     3781 aacggacgca gtacgacctg gagatgatgc gggagctggg atattgctcc ggcatcgaga
     3841 actactctcg gcatatggac gggcgtcccg ccggctccac gccgtacacg ctgctggatt
     3901 acttccccga tgactacgtt acgttcgtgg acgaatctca cataacgctg ccccagatcc
     3961 ggggcatgta caacggcgac cggagccgca agacgacgct cgtcgagttc gggtttcggc
     4021 tgccaagcgc gctggacaac cggccgctta tgtgggagga gttcctgctc aagacggatc
     4081 agatggtctt cgtgtcggcg acgccggggc cgtatgagct ggagaactcc ggtcagatag
     4141 tcgagcagat catccgcccg accggcctca tagacccgga ggtagaggtc cggccgacga
     4201 agaaccagat cgacgacctg ctcaacgagg tcgccaagcg cgtcgagcac ggcgagcgcg
     4261 tcctgatcac cacgctcacc atcaagatgt ccgaggacct gacggactac ctgctggagc
     4321 acaacgtcaa ggcgcgctac atgcactcca acatagagac gctggaccgg atccagatca
     4381 ttcgtggatt gaggaccggc gagttcgacg tgctcgtcgg catcaacctg ctccgggagg
     4441 gactggacct tccggaagtc acgctcgtcg ccatcctgga cgcggacaag gagggcttcc
     4501 tgcgcgggga gcgggcgctc atccagacca tcggtcgggc ggcgcggaac gtcaacggcc
     4561 gggtcatcat gtacgccgac cgggagaccg attccatgcg gctcgcaata ggggagacca
     4621 accgccggcg cgagatacag accgcccaca acgagcgcaa taacatcgtg ccgcgcacca
     4681 tcagcaaggg cgtctccgac atcctcat
//
LOCUS       DASSMB010000099         5777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1159360, whole genome shotgun sequence.
ACCESSION   DASSMB010000099 DASSMB010000000
VERSION     DASSMB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159360"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..709
                     /locus_tag="VFJ72_06435"
     CDS             131..709
                     /locus_tag="VFJ72_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7479136.1"
                     /translation="MPEEGEAIREPASAERRYYHPLQRDYATFLKTSEETGGEFTLIE
                     IELAPCGGNALHYHKAFDEHFEALEGALEVQTGEERRILHPGEKATAAKNVLHCFRNP
                     TDEPARFLVELRPGHTGFENALKTGYGLASDGLARANGAPKNLYYLALLFEWSDSRLP
                     GFFAVAEPLFRFLARRARRKGIDEYLEERYCR"
     gene            808..1275
                     /locus_tag="VFJ72_06440"
     CDS             808..1275
                     /locus_tag="VFJ72_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479137.1"
                     /translation="MRGSVGETLERLILLQIGAAAATRDKVQEVVESLIQQGRIEREE
                     GRTLVNDVVDRARERSAGARSLFDASMQQGLRGAGVPTREDYEDMVFRVEQLEHRVRM
                     LEGATSAPPPPRAGEPEAPPEPPPAGHPPTPPLAGVDDPPPDSRDAPGTPPGL"
     gene            1297..2976
                     /locus_tag="VFJ72_06445"
     CDS             1297..2976
                     /locus_tag="VFJ72_06445"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HET7479138.1"
                     /translation="MTPGGMPPPVPVPRTGNLARFSQIGRVMVRHGFGFVFDVRRDRR
                     DKKGLQEMLGPNFGVRLRKTLDDLGPTFVKFGQLLSTRSDILPESVLFELQKLQDTVA
                     PMPLESAQEIIQRELGAPVGEVFAEFDVVPLGSASIGQVYRAVLRSGESVAVKVQRPE
                     APRRVEADLEIMRDFASFLDRRFGRRIFIDVRGLVAEFEGVIRRELDYTAEAGNARRF
                     AINFAETPVVIPGIYLDLSTPRVLTMEYIEGTRFHDIRPLSLLPRERRRVAGMGAEAI
                     FKMAFEDGFFHGDPHPGNLILTPEGDLALLDFGMVGFMSRGDIDALSRLFIAVIQRDA
                     AAALRGLEGLGVTYASEVRGDLIQDLREFLFKYSGLSVGEVTLGQALSELISLARRYR
                     MRVPPVFPLLTKALVTAEGLSRSIDPTLNVYEVARPYAQRLLSERYEPENVLEGARER
                     ALEYARYLEDYPEQIRLLLHELEDGELEVKLRHRGLDELGGEVDVLANRLVFAVVTGA
                     LLIGSSMLGAFNRGGPQVPYLGVPLISFLGFMLALIMASIVLVIIFRSRRL"
     gene            3024..5255
                     /locus_tag="VFJ72_06450"
     CDS             3024..5255
                     /locus_tag="VFJ72_06450"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7479139.1"
                     /translation="MESAHIEHLKAVPTPTLTISSLLEKVRGYSPEGAEQLVAEAYNV
                     SHAAHRGQVRKSGEPFVYHPLATADILADLHLDATTVAAALLHDVLEDTEITKEELAR
                     RFGDELAEIVDGVTKLKRLPSGNLEEAQAESLRKMIVAMSKDVRVIIIKLADRLHNMR
                     TLAYLKRETQLRKATETLEIYAPLAHRLGIYSVKWELEDLSFATLHPRRYEEIKRLVA
                     ARRGDREAFINGTAAELLRHLSQTGIEDAEVHGRVKHFYSIYNKMVRRNKEFNEIYDL
                     AGLRVIVNNVRDCYGALGVIHSIWKPIPGRFKDYIAMPKFNMYQSLHTTVMSNEGKLL
                     EIQIRTHDMETTAEYGIAAHWMYKHGLREGEVDRLTWLKSMMEWQKETTDSSEFMESL
                     KGELVADEVFVFTPKGDVVSLPAGATPIDFAYHVHTEVGHRTIGAKVNGRIVPLDSEL
                     VSGDRVEIITGKSASPSRDWLTVVQSGRARNKIRQFFNKADREDNLSLGREKVVGLLK
                     KQRIGKVPSGILEDVARMVNYPSPDDMLAAVGSGALSGENVAHRIAERMQPPKEESEV
                     KARPKSPALTPLPLPESKTDEETGVRVVGSSGILTRLARCCTPMPGDDILGYVSLGRG
                     VTVHSAGCANARALKARDPDRFVEVEWAHGPNRLFTVELLVEALDRMHLLKDITSTIS
                     DAGVNILSARVDTIEDRTALSRFAFRAASVQHVDEVIRKIRSIPDVYDVYRVSRDGTP
                     LEN"
     gene            5261..>5777
                     /locus_tag="VFJ72_06455"
     CDS             5261..>5777
                     /locus_tag="VFJ72_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7479140.1"
                     /translation="MAEIEQVQLTMGGFEVNSYVIHGPEGDIIVDAGAEPEKILDALD
                     APAAILITHNHGDHIAALEDVRRETNVPVYMHPVDADAAGVRDYEPLGDGDELALAGL
                     GLRVLHTPGHSPGSVTFVVGEDQIVGDLILPGSVGRTDLSGASWEEIEVSIRKVMPLW
                     TDATRLFTGHGP"
BASE COUNT         1040 a         1825 c         1968 g          944 t
ORIGIN      
        1 ggttcttcct cgaaaatacg ctaaacgggt gatacgcctc gcccgatagg tggatacgat
       61 gcgctcaagt gcaaaaggtg gcgtttctcg tcgtcgtgcc ggagcgcctg agaaaagaga
      121 agaggccgat atgcccgaag agggagaggc gatccgggag ccggcgagcg ccgagaggcg
      181 ctactaccat ccgctccaga gagactacgc cacgttcctg aagactagcg aggagacagg
      241 cggcgagttt acgctcatcg agatagagct tgcgccctgc ggcggcaacg ctctccacta
      301 tcacaaggct ttcgatgagc acttcgaggc gctggaaggc gcgcttgagg tgcaaacagg
      361 tgaagagcgg cgcatactcc atcccggaga gaaggcgacg gccgcgaaga acgtcctgca
      421 ttgctttcgc aatcccaccg atgagccggc caggttcctg gtcgagttgc ggcccggtca
      481 caccgggttc gagaacgcgc taaagaccgg ctacgggctg gcgtccgatg gtcttgcccg
      541 cgccaacggc gcacccaaga acctttatta tttggccctt ctgttcgagt ggtcggatag
      601 ccgcctgccc ggcttcttcg ccgtcgccga gccgctgttt cggtttctgg cgcggcgtgc
      661 ccggcgcaag gggatcgacg agtatctaga ggaacggtac tgccggtagg gacgtctccg
      721 ggcggcaagc atcaaacaag ccagcctcgg cctctcccga gtcgcgttat catgtaggca
      781 ctggacccca gttttcggga ggtagctatg cgtggatcgg ttggcgagac gctggagcgg
      841 ttgatcttgt tgcagatagg ggctgcggct gcgacgcggg ataaggttca agaggtcgtc
      901 gagtccctga tccagcaggg ccgcatcgag cgcgaggagg gccgcacgct ggtgaacgac
      961 gttgtggacc gggcccgcga gcgctccgcc ggtgcccgct cgctcttcga cgcctcgatg
     1021 caacaggggc tgcggggagc gggcgtaccc acccgcgagg actacgagga catggtcttc
     1081 cgggtcgagc aactcgagca ccgggtgcgg atgctggagg gagccacctc cgcgccgcct
     1141 ccgccgcggg ccggtgaacc ggaggcgccg ccggagccgc cgcccgccgg acaccctccg
     1201 acgcctccgc tggccggcgt ggacgacccg ccaccggatt cccgcgacgc tccggggacg
     1261 cctcctggcc tttaacggaa gcgcagagag ccgcccgtga cgccgggggg catgccgccg
     1321 ccagtgccgg tcccgcgcac gggtaacctg gcgcggttct cgcagatcgg gcgcgtcatg
     1381 gtccggcacg gcttcgggtt cgtcttcgat gtccggcgcg accggcgcga caagaagggt
     1441 ctgcaggaga tgctcggccc gaacttcggg gttcgtctgc gaaagacgct ggacgacctc
     1501 ggcccgactt tcgtgaagtt cggccagcta ctcagcacgc gctcggacat cctcccggag
     1561 agcgtgctct tcgagttaca gaagctccag gatacggtcg ccccgatgcc cctggagagc
     1621 gctcaggaga taatccagcg cgagctcggc gcgccggtgg gagaggtctt cgccgagttt
     1681 gatgtcgtgc cgctcgggtc ggcgagcatc gggcaggtgt accgggcggt gcttcggagc
     1741 ggggagagcg tggccgtcaa ggtgcagcgc ccggaggccc cgcgccgggt cgaggcggac
     1801 ctggagatca tgcgcgactt cgcttccttc ctggaccggc gcttcgggcg gaggatcttt
     1861 atagacgtgc gggggctcgt ggcggagttc gagggcgtta tccggcgcga gctcgactac
     1921 accgccgaag ccggaaacgc ccggcgcttc gctatcaact tcgccgagac gccggtcgtg
     1981 atacccggca tctacctcga tctctccacg ccccgcgtcc tcacgatgga gtacatagag
     2041 gggacgcgct tccacgacat ccgtccgctc tcgctactcc cccgcgagcg gcggcgcgtg
     2101 gccgggatgg gggcggaggc tatcttcaag atggccttcg aggacggctt cttccacgga
     2161 gacccgcacc ccggaaacct catcctcacc ccagaagggg acctggcgct gctggacttc
     2221 gggatggtcg gcttcatgag ccggggcgac atagacgccc tgagccgcct gttcatagcc
     2281 gtcatccagc gcgacgcggc ggcggcattg cgcgggctcg aaggtttagg ggttacctat
     2341 gcgtcggagg tgcggggaga tttgatccag gacctccgcg agttcctgtt caagtattcc
     2401 gggctctctg taggggaggt gacgctcgga caggcgctct cggagttgat ctctctggct
     2461 aggcgctacc ggatgcgggt cccgcccgtc ttccccctgc tgacgaaagc tcttgtgact
     2521 gccgaagggc tctcacgctc catagacccg acgttgaacg tgtacgaggt cgcgcgtccc
     2581 tacgcccagc ggctcctctc cgaacgctac gaacccgaga acgtcctgga aggagcgcgc
     2641 gagcgggcgc tggagtacgc ccgctacctg gaggattacc cggagcagat ccggctcctg
     2701 ctccacgagc tcgaagacgg cgagctggag gtgaagctgc gccaccgggg cctggacgag
     2761 ctcggcggag aggtggacgt gctcgccaac cggctcgtct tcgccgtcgt aaccggcgcg
     2821 ctactgatcg gctcctccat gctcggggcg ttcaaccgcg gcgggccgca ggtcccgtac
     2881 ctcggcgtgc cgctcatcag cttcctcggg ttcatgctcg ccctgataat ggcctctata
     2941 gtgctggtca tcatcttccg ctcccgccgc ctctagcgcc ggctggccga aatgccctcg
     3001 tcgggacagg atataatcct ggtatggaga gcgcgcacat agagcacctg aaagccgtcc
     3061 cgacaccgac tctgaccatc tcctcgcttc tggagaaggt gcggggctac agtccggagg
     3121 gcgccgagca gctcgtcgcc gaggcgtaca acgtctcaca cgcggcgcat cgcggccagg
     3181 taaggaagag cggggagccg ttcgtctacc atccgctggc gacggcggac atcctcgccg
     3241 acctgcacct ggacgccact acggtggcgg cggcgctgtt gcacgacgtg ctggaggaca
     3301 cggagatcac caaagaggag ctcgcccgac gcttcggaga tgagctggcg gagatcgtgg
     3361 acggcgtcac caagctgaag cggctcccca gcgggaacct ggaggaggcg caggccgagt
     3421 cgctgcggaa gatgatcgtc gcgatgagca aggacgtgcg cgtcatcatc atcaagctcg
     3481 ccgaccggct ccacaacatg cggaccctcg cctacctgaa gcgcgagacg cagctaagaa
     3541 aagcgacgga gacgctggag atctacgctc ccctcgctca ccggctgggt atctactccg
     3601 tgaagtggga gctggaggac ctatcgttcg ccacgctgca cccgcgccgc tacgaggaga
     3661 taaagcggct cgtggccgcg aggcggggcg accgggaggc gttcatcaac gggacggcgg
     3721 cggagcttct gaggcacctg agccagaccg gcatcgagga cgccgaggta cacgggcggg
     3781 tcaagcactt ctactccatc tacaacaaga tggtccgcag gaacaaggag ttcaacgaga
     3841 tctacgacct cgccgggctg cgggtgatcg tgaataacgt gcgcgactgc tacggcgctt
     3901 taggcgtcat acattcgatc tggaagccga taccgggccg gttcaaggac tatatcgcca
     3961 tgcctaagtt caacatgtac cagtcgctgc acacgacggt catgtcgaac gaggggaagc
     4021 tgctggagat ccagatccgg acccacgaca tggagacgac cgccgagtac gggatcgccg
     4081 cgcactggat gtacaagcac ggcctgaggg agggcgaggt tgaccgcctg acctggctca
     4141 agagcatgat ggagtggcag aaggagacca ccgactcctc ggagttcatg gagtccctga
     4201 agggcgagct cgtcgccgat gaggtcttcg tcttcacgcc aaagggcgat gtggttagtc
     4261 tgccggccgg ggcgacgccg attgacttcg cctaccacgt ccatacggag gtcggccaca
     4321 ggaccatcgg ggccaaggtc aacggccgta tcgtgccgct tgactcggaa ctggtgagcg
     4381 gtgatcgggt ggagatcatc accggcaagt ccgcgagtcc ttcccgcgac tggctgacgg
     4441 tcgttcagag cgggcgcgcc cggaacaaga tccggcagtt cttcaataag gccgaccggg
     4501 aggacaacct ctctctagga cgcgagaagg tcgtggggct gttgaagaag cagcggatcg
     4561 ggaaggtccc ctccggcatc ctcgaagacg tggcgcggat ggtcaactac ccgagcccgg
     4621 acgacatgct cgccgccgtg gggtcgggcg cgctctccgg cgagaacgtc gcgcaccgca
     4681 tcgccgagag gatgcagccg ccgaaggagg agagcgaggt gaaggcgcgg cccaagagcc
     4741 cggccctcac gccgctacct ctccccgaga gcaagaccga tgaagagacc ggcgtgcggg
     4801 tggtggggtc tagcggcatc ctcacgcggc tggctcgctg ctgcaccccc atgccgggcg
     4861 acgacatcct gggttacgtc tcactgggac gtggcgtgac ggtccactct gcgggctgcg
     4921 cgaacgcgcg ggcgctcaag gcgcgcgacc cggaccgctt cgttgaggtg gagtgggcgc
     4981 acggcccgaa ccggctcttc acggtggagc tgctcgtcga ggcgctcgac cggatgcacc
     5041 tgctaaagga catcacctcg accatctccg acgcgggagt gaacatactc tccgcgcggg
     5101 tggacaccat agaggaccgg acggccctca gccgtttcgc gttccgggcc gccagcgtgc
     5161 agcacgtgga cgaggtcata cggaagattc gctccatccc cgacgtctac gacgtgtacc
     5221 gcgtctcgcg cgacggcacg cccctggaga actagacgag atggccgaga tagagcaggt
     5281 tcaactgacc atgggcggct tcgaggtcaa ctcctacgtc atccacggac ccgaaggcga
     5341 catcatcgtg gacgcgggcg cggagccgga gaagatcctg gacgctctcg acgcgcctgc
     5401 cgccatcctg atcacgcaca accacggcga ccacatcgcc gccctggagg acgtgcgccg
     5461 ggagacgaac gtcccggtct acatgcaccc cgttgatgcg gatgccgctg gcgtccggga
     5521 ctacgagcct ctcggcgatg gagatgagct tgcgctggca gggctgggtc tcagggtgct
     5581 gcacacgccg ggtcattcgc cgggctccgt gacgttcgtg gtcggcgaag accagatcgt
     5641 gggagacctc atcctgccgg ggagcgttgg acggacggac ctctcggggg cgtcgtggga
     5701 ggagatcgag gtctccatcc gcaaggtgat gccgctgtgg acggacgcca cgcgcctctt
     5761 tacgggacat ggcccgg
//
LOCUS       DASSMB010000100         5230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1159891, whole genome shotgun sequence.
ACCESSION   DASSMB010000100 DASSMB010000000
VERSION     DASSMB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159891"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1207
                     /locus_tag="VFJ72_06460"
     CDS             158..1207
                     /locus_tag="VFJ72_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET7479141.1"
                     /translation="MALNEVLLKRLIETPGVSGREEKQREISREELSSLTDEVRTDSL
                     GNVIGTKKGNADVRVMVAAHTDEIGFLVRHIDDKGFLRLQTLGGHDPANMVSQRVLVV
                     TADGSTFRGALQPARKPPHLARGEEQKLPKADEFYVDLGMSAEEVKERVRVGDYAVMD
                     RTLEKVGGNYMGKAMDDRIGVFVMLEALREMGEHEATVFAVATSQEEVGLRGAAASGA
                     ALEPTVVVALDITLAMDVPGGGNENEISALGKGAAIKIMDASLISHPKLVEHFREIAE
                     REGIPHQMEILPFGGTDAGGVQRLHGGIPAITLSVPCRYVHTVNEMVSAEDVQACITL
                     LARYLEEAHTGDYAL"
     gene            1209..1895
                     /locus_tag="VFJ72_06465"
     CDS             1209..1895
                     /locus_tag="VFJ72_06465"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HET7479142.1"
                     /translation="MGVTRKTISVLFLIGVNMVPLLGVIFYDWNLFSIMFLYWFENGI
                     IGLFNVLKISRAAKPSSGNTNLTINGRPTNLSGKAFYVPFFIMHYGIFWVVHGVFVIV
                     LFGLFGGQPFGSVGPPEIMGGFRGFEPGGVAIATLSLFVSHGVSFFVNFLGNEEYLNV
                     SPDEQMFQPYGRVIALHITILGGGFLAGYLGTPLASLIILVAVKTAIDIWAHLREHRK
                     AEDPTTPPTV"
     gene            1960..2145
                     /locus_tag="VFJ72_06470"
     CDS             1960..2145
                     /locus_tag="VFJ72_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479143.1"
                     /translation="MEYVTDEHGKRTRVILEIKEYERLVEAAEDAEDIHEHERVMAAI
                     RNGEEERIPFERKPADS"
     gene            2159..2416
                     /locus_tag="VFJ72_06475"
     CDS             2159..2416
                     /locus_tag="VFJ72_06475"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7479144.1"
                     /translation="MARSADRALKKLPIRDRERVDQRIDALAAAPRPPQSTRLVGPNP
                     RAYRLRQGDYRIVYEVDDEALEVTVTRVAHRRDVYRRLDLT"
     gene            complement(2473..3153)
                     /locus_tag="VFJ72_06480"
     CDS             complement(2473..3153)
                     /locus_tag="VFJ72_06480"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7479145.1"
                     /translation="MKITFYAHASFRLEADGLSVVTDPYTPGPRDSNFDPIDEPADLV
                     IMSSATDRFHSDPSHVRGDPTVVNALELPPEGKEVLGLHIRPFPAMESMTFDFGRDPD
                     ANAMYLFTLGGLRVLHIGDIGNPVPQAHLDALRENVDLLFALAGAHATIALEDLDDAI
                     SAIGPRVIIPMHYHHPRGRLEIEPVETFLQSFPAASVTRVSGCELELTPESLPADAPH
                     VYVLEQSR"
     gene            3302..3823
                     /locus_tag="VFJ72_06485"
     CDS             3302..3823
                     /locus_tag="VFJ72_06485"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7479146.1"
                     /translation="MAANSYSNPPEMALKPGAEYYANFITERGAVKVRLFAEEAPETV
                     NNFVFLAQDGYFDGTTFHRVIKDFMVQGGDPTGTGTGGPGYRIRDEFHPELRHDRPGI
                     LSMANAGPNTGGSQFFITHVATPWLDDRHAVFGEVVEGMDVVEAIRERDPQRDREPGD
                     RIERVEIEETQRS"
     gene            complement(3903..4313)
                     /locus_tag="VFJ72_06490"
     CDS             complement(3903..4313)
                     /locus_tag="VFJ72_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479147.1"
                     /translation="MRRATMILSIGVLLLALFVPGALAATGCASIFSGTDGPDTLRGD
                     RCDNNISGLGGNDIIYGYGGDDELFGNQGDDLLRGGKGRDKLYGGSGNDRIFSGGLDG
                     KRDRVVCGSGRDFASIAENDLAAKDCEVVAVAVE"
     gene            4515..4898
                     /locus_tag="VFJ72_06495"
     CDS             4515..4898
                     /locus_tag="VFJ72_06495"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7479148.1"
                     /translation="MSREVADRFVEALWRLEEESDVEPLVEVHTDDCDVGNVAVPKNF
                     SGHEGLREFWSSYRKTFGEMKSEFRNVFADGDGRAALEWTTEGTSNGGAISYEGVSVL
                     EIEGDKVRRFYAYFDPRSLTEQVID"
     gene            4938..>5230
                     /locus_tag="VFJ72_06500"
     CDS             4938..>5230
                     /locus_tag="VFJ72_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479149.1"
                     /translation="MGGPGSITDETSVRDLTRARRILVVGPGGTGKSTLALRLGEILH
                     LEVIHLGRFYWSAGWVQPPPDEWETTVRRLVNRESWIIDGNYGGTLDLRLAAA"
BASE COUNT         1005 a         1590 c         1744 g          891 t
ORIGIN      
        1 gcctgagcct ctacctctcc ggcaagctcc tcgaagcccg ccgcctcgaa caagaggcga
       61 tggagtaaca aacctgctcc gcccggcgcc actctttgac gcgcccgcac gccgttgcta
      121 ggctcaccgg acgatccgcg aaaagagagg attagtcttg gcactcaacg aggtgttgct
      181 gaagcggttg atcgagactc caggggtatc cgggcgggag gagaaacagc gcgagatctc
      241 gcgcgaagag ctgtcttccc tgaccgatga ggtccgcacg gactccctgg gcaacgtcat
      301 cggaacgaag aagggcaacg cggacgtccg cgtcatggtc gcggcgcaca ccgatgagat
      361 cggtttcctc gtcaggcaca tagacgataa gggctttctc cggctacaga ccctcggcgg
      421 acacgacccg gcgaacatgg tctcccagcg cgtcctggtc gtgacggcgg acggctcgac
      481 gttccggggc gccctgcaac cggcgcgcaa gccgccgcac ctggcgcgcg gcgaggagca
      541 gaagctcccg aaggccgacg agttctacgt ggacctcggc atgagcgcgg aggaggtgaa
      601 ggagcgggtc cgcgtcgggg actacgcggt gatggaccgg acgctggaga aggtcggcgg
      661 caactacatg ggcaaggcga tggacgaccg catcggggtc ttcgtgatgc tggaggcgct
      721 gcgggagatg ggcgagcacg aggcaacggt cttcgcggtg gcgacttcgc aggaagaggt
      781 aggattgcgc ggggcggcgg cgagcggggc ggcgctggag ccgacggtcg tggtcgcgct
      841 ggacatcacg ctggcgatgg acgtgccggg cggagggaac gagaacgaga tctccgctct
      901 cggcaagggc gcggcgatca agataatgga cgcctcgctc atctcccacc cgaagctcgt
      961 cgagcacttc cgcgagatcg cggagcgcga gggcatcccg catcagatgg agatactgcc
     1021 cttcggcggc acggacgcgg gcggggtgca gcggctgcac ggcggcatcc cggccatcac
     1081 gctctcggtg ccgtgccgct acgtccatac cgtcaacgag atggtgagcg ccgaggacgt
     1141 gcaggcttgc ataacgctgc tggcccggta tctggaagag gcccacaccg gagattacgc
     1201 gctctaagat gggcgtcacc cgcaagacca tttcggtact gttcctcatc ggggtgaaca
     1261 tggtccccct cctcggggtg atcttctacg actggaacct gttctcgatc atgttcctgt
     1321 actggttcga gaacgggatc atcggcctgt tcaacgtact caagatctcg cgcgccgcca
     1381 agccatcgtc cggcaacacg aacctgacca tcaacggccg ccccacgaac ctgtcgggca
     1441 aggcgttcta cgtcccgttc ttcatcatgc actacggcat cttctgggtg gttcacggcg
     1501 tcttcgtgat cgtgctcttc ggcctcttcg gaggccagcc gttcgggagc gtgggaccgc
     1561 cggagatcat gggcggcttc cgaggcttcg agcccggcgg ggtcgccatc gccaccctct
     1621 cgctcttcgt gagccacggc gtctcgttct tcgtcaactt cctgggtaac gaggagtacc
     1681 tcaacgtctc gccggacgag cagatgttcc agccttacgg gcgcgtgatc gcgctgcaca
     1741 tcaccatcct cggcggcggc ttcctcgccg gttacctggg caccccgctc gcctccctca
     1801 tcatcctggt agcggtcaag accgccatag acatctgggc gcacctcagg gagcaccgca
     1861 aggcggagga tccgaccacc cctccgaccg tttagtctgg cagtctccag acggctcgga
     1921 aggcgttaag ataaaggcaa gcatctcaaa ggaggtgaca tggagtacgt tacggacgaa
     1981 cacggcaagc gcacgcgagt gatactggag atcaaagagt atgaacggct cgtcgaagcc
     2041 gccgaagacg cggaggacat acatgagcac gagcgagtaa tggccgccat caggaacggc
     2101 gaggaagaac gcataccctt cgagcgaaag ccggctgata gctgagtacg cggtattcct
     2161 ggcccgttcg gcagacaggg ctctgaagaa actccccatc cgggaccggg aacgggtaga
     2221 tcagagaata gacgctctcg ccgccgcccc gcgcccgccg caatctacga gactcgttgg
     2281 acccaacccg agagcctacc ggttgcgtca gggcgactac cggatcgtct acgaggttga
     2341 tgacgaagcc ctggaagtaa ccgtcaccag ggtcgcccac cgcagagacg tctacagacg
     2401 tctggacctt acatagtcgg cgcaagcatg gtgctgctcg atacctcgaa gaagcgaata
     2461 tgggggatta cgtcaacgcg actgctccag cacgtagacg tgcggcgcgt cagcgggcag
     2521 cgactcgggc gtgagttcga gttcgcaacc gctgacccgc gtgacggagg ctgccgggaa
     2581 gctctggagg aaggtttcta ccggttcgat ctccagcctg ccgcgcggat gatggtagtg
     2641 catcgggatg ataacgcgcg gaccgatggc ggagatggcg tcgtcgaggt cttccagcgc
     2701 gatggtcgcg tgggcgcccg ctagagcgaa gagcaagtct acgttttcgc gcagcgcgtc
     2761 gaggtgcgcc tgcgggaccg ggttgccgat gtcgccaatg tgcaagacgc gcaggccgcc
     2821 gagcgtgaac aggtacatgg cgttcgcgtc agggtcgcgc ccgaagtcga aggtcatgct
     2881 ctccattgcg gggaaggggc ggatgtggag gcccaggacc tccttgccct ccggtggaag
     2941 ctccagcgcg ttgacgaccg tggggtctcc gcgcacgtgg ctcgggtcgg agtggaagcg
     3001 gtcggtggcg gagctcatga tcacgaggtc cgccggttcg tctatagggt cgaagttgga
     3061 gtcccgcggt ccgggcgtgt aggggtcggt gacgacggag agcccatcgg cttcgagacg
     3121 gaagctcgcg tgggcgtaga acgtgatctt cactggcgtc ctcctgagat gatcttgtgg
     3181 cgttcagaat acccgcgaac ccgaaacgcc gcaggtcggc gcggcccgca gggtccacgt
     3241 gcccgaagat gcggtagcat aggcgcttgc cgttacagcg agaagagccg gaggagatcc
     3301 gatggcagca aacagctaca gcaacccgcc cgagatggcg ctgaagccgg gcgcggaata
     3361 ttacgcaaac ttcatcacgg agagaggcgc cgtcaaggtg cgtctcttcg ccgaggaggc
     3421 gccggagacg gtgaacaact tcgtcttcct ggcgcaggac gggtacttcg acgggacgac
     3481 cttccaccgg gtcataaagg acttcatggt gcagggcggc gacccgacgg gcacggggac
     3541 tggtggtccg gggtatcgga tcagggacga gttccatcca gagctccggc acgaccgtcc
     3601 gggcatcctc tcgatggcga acgcgggacc gaacacgggc ggctcgcagt tcttcatcac
     3661 gcacgtggcg acgccgtggc tggacgaccg gcacgcggtc tttggggagg tcgtcgaggg
     3721 gatggacgtg gtggaagcca tccgggagcg cgacccgcag cgcgatcggg agccgggaga
     3781 caggatcgag cgagtcgaga tcgaggagac ccagcgctcc tagcggcgag taggccgggc
     3841 tccgggcgga tcgtccggag cccaggatct acagttccta acgagcctgc cgggtgtcgt
     3901 tctcactcca ccgctaccgc caccacctcg cagtcctttg cggcgaggtc gttctcggcg
     3961 atggaagcga agtcccggcc actgccacag acgacgcggt ctcttttccc gtcgaggccg
     4021 ccgctgaaga tcctgtcgtt tccggagccg ccgtagagtt tgtcccggcc cttgccgccg
     4081 cggagtaggt catcgccctg gttgccgaaa agctcatcgt cgcccccgta accgtagatg
     4141 atgtcgttcc cgcccagtcc gctgatgttg ttgtcgcagc gatctcccct gagagtatcg
     4201 ggtccgtcgg taccgctgaa tatactcgcg cagccggtcg ccgccagcgc tcccggcacg
     4261 aagagagcca gcaagagcac tcctatagac aggatcatcg tcgctcgcct catctctctc
     4321 ctttccgaga aatggctgat caccctacag cttcatggtg ctctcagcag ataacgggcg
     4381 catcatccga acggcttatc tggcgttccc agaaggatga tccttcccgg agttgagtgc
     4441 gggtttacag tctccctgcg gaggtagccg ctatcctcgt gaacacgtgc aatccatgag
     4501 agaggtgtaa ccccgtgtcc agagaggtag cagacaggtt cgtcgaggcg ttgtggaggc
     4561 tcgaagaaga gagcgacgtg gagccgctgg tcgaggtcca caccgacgac tgcgacgtgg
     4621 gcaacgttgc ggtcccgaag aatttctccg gccatgaggg gctgcgcgag ttctggagca
     4681 gttaccgcaa gactttcgga gagatgaaga gcgagttccg taacgtcttc gccgacggcg
     4741 acggccgcgc cgccctcgaa tggactaccg agggtacatc caatggcggc gccatctcct
     4801 atgagggcgt gagcgtcctg gagatagaag gcgataaggt ccggcggttc tacgcctact
     4861 tcgaccctcg cagcctgacc gagcaggtca tagactagcc tccccgaaag ccgccaccat
     4921 ggcggtagaa tccccgcgtg ggcggtcccg gaagcataac ggacgagaca tcggtgcgag
     4981 atctcacacg ggcgaggagg atcctcgtcg tcggtccggg cggtactggc aagagcacgc
     5041 tcgcgttgcg gctcggagag atcctgcacc tggaagtcat ccacctgggc cgcttctact
     5101 ggagtgcggg ctgggtccag ccccctcccg acgaatggga gacaacggtg cgccggctcg
     5161 tcaatagaga atcctggatt atagacggta actacggcgg caccctggac ctgcggctgg
     5221 ctgccgcaaa
//
LOCUS       DASSMB010000101        13723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1542368, whole genome shotgun sequence.
ACCESSION   DASSMB010000101 DASSMB010000000
VERSION     DASSMB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13723
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542368"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFJ72_06505"
     CDS             <1..357
                     /locus_tag="VFJ72_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7479150.1"
                     /translation="TTIASSVVSSIVNVAAGEVDGLAMSHGGTRLPGDNSPTVGEFFD
                     RVSGGGSRTRGVSVEVGEREAAVDLTVNVVYGKPIAQVTQSVRQNIIRRVESLTGLDV
                     TEVNITVNDVTFPEQQ"
     gene            complement(472..1806)
                     /locus_tag="VFJ72_06510"
     CDS             complement(472..1806)
                     /locus_tag="VFJ72_06510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET7479151.1"
                     /translation="MPEVIMPKMGDAMEEGTLLKWLKSEGDEVSEGDAIAEIETDKVT
                     MELEAEDAGTLAELIASEGQEVPVGEAIAFIQGEGEEAPERSGGGSAEAAEEEGGEAK
                     AETATQTEAPPEGGDARADGKGDGRANGQFRASPIVRRLADENDLDLSKIDGSGPAGR
                     IVERDVRAAMESGSAKRDGGEAGAAAADGAAEAQPQQAAQAGFQAQKLPEPTGAPGTT
                     MVEPTKMQRIIGDRMTEAKQHIPHFYATVEVQMDAAMALRKQLNAQLEADGIKLSVND
                     FVMKACAVALRNYPNLNALYTSKGIELHETVNMAMAVALDAGLITPVIKNIESKGLAT
                     ISRESKDLATRAREGGLKPDEYQGGTITVSNMGMFGVDSFTAIINPPQASIIAVSSIV
                     KRPSYDENGEIVPASFMKLTLSADHRIANGRDGALYMSEVKKTLENPVLLMV"
     gene            complement(1808..2800)
                     /locus_tag="VFJ72_06515"
     CDS             complement(1808..2800)
                     /locus_tag="VFJ72_06515"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7479152.1"
                     /translation="MAETKTYREALRDGMTHEMDRDESVVLMGEDIGVYGGTHLITDG
                     LLDQYGPKRVMDTPIAEGGFTGAAIGMAMLGMKPVVEMMTWNFSFLASDQIIQNAAKV
                     RYFSGGQVEVPLVIRGPNGGGVQLSAQHTHSLENLYGHFPGLKVVSPVTPNDVKGMMI
                     TAIRDPNPVIFLEAGALYGTKGEVEEGDNAVEFGKARVAREGSDVTLIAYGRQVNLCL
                     RVADTLAEEDDVQAEVIDIRSIRPFDEETIVESVKKTHRAVTVQEQWRWFGVASEVAA
                     IIQEQAFDYLDAPVMRVSGAEVPAPYARNLELAAFPSEKAVANAARRALYIEEK"
     gene            complement(2804..3877)
                     /gene="pdhA"
                     /locus_tag="VFJ72_06520"
     CDS             complement(2804..3877)
                     /gene="pdhA"
                     /locus_tag="VFJ72_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HET7479153.1"
                     /translation="MSADNEHSENGDVGAREESGSEGAVATSTDRLVDIYREMAKIRA
                     FEEACQKGFRQGKIGGYLHVYIGQEAVATGFLQAYKEGDKVITAYRDHAHALLLGTDP
                     KAVMAELYGKGTGMVKGKGGSMHLFDVERGLMGGYGIVAGHIPLGVGFAYAMRYQETD
                     HITQLYLGDGSMSNGAFHEAANLAGLWGKDGMCPCLFIVENNKYGMGTSVERTTAMTD
                     LAAKFDAYGIENEKVDGMDIEAVIECAERVTEQVRETGKPYAVEALTYRTVSHGAADF
                     MEKYRTKEEVNEWRQRDPIGLLEHKLIENDILDEGKLDEIKSETKEYVAEVVKFADES
                     DEPPIEELYTDVYAGADEYMGEE"
     gene            complement(3979..4545)
                     /gene="rdgB"
                     /locus_tag="VFJ72_06525"
     CDS             complement(3979..4545)
                     /gene="rdgB"
                     /locus_tag="VFJ72_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET7479154.1"
                     /translation="MGNQRAVFVTSNPNKRREAATILGLELDRADLDLPEMQALDVAE
                     VAAAKARAAREALGFPSRPVIVEDSGLIMESWNGLPGALTKWFIKSVGHEGVLRMLGA
                     VEDRRARAVCAVAVADGDGEVRVFVGEVAGEVVSEPRGESGFGWDPIFAPEGSSLTYA
                     EMGEAKNEDSHRARAFRAAREWLIAGRE"
     gene            complement(4617..6002)
                     /locus_tag="VFJ72_06530"
     CDS             complement(4617..6002)
                     /locus_tag="VFJ72_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7479155.1"
                     /translation="MSVTKELRTLAENGASGDRLPSVRELMSRHGAGPATVQRAVSRL
                     VGEGLIEARPGRGSFVAPRREVLEPADLGWQAVALGERGVSAEGLDELLAVPAPGVLP
                     LTAGYLPEDLQPLGRLSAAMTRAARRPGAWGRVPVEGVVELRAWFAREVGGAFGASDV
                     VVTSGGQAALSVAFRALASPGSPVLMESPTYIGAIAAARAAGLRPVPVPTDSGGVRLD
                     LLLEAFASSGARLFYCQPTYANPHGAVLDRERRKAVLEVARKANAFVIEDDWARDFAL
                     EGDPPTPLAAHDPDGHVVYVRSLTKSLAPGMRVGALCARGAAGERLKAARIVGDFFVA
                     GPLQEAALEVLSSPAWPRHLRHLRKVLKSRRDALVASVREGLPVARLTLVPDGGLHLW
                     VRLPDELDDVLVAEGAMRAGVMVSAGRRWFPAEPTGSFLRLSYVGAETGVLAEGVQRL
                     NRVLEAQTPRG"
     gene            6022..6930
                     /locus_tag="VFJ72_06535"
     CDS             6022..6930
                     /locus_tag="VFJ72_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7479156.1"
                     /translation="MDIKSNVTGITSETQGLIFGGLGVLAFSFTVPLTRVAVGELDAT
                     FVGLGRALVAALLAAVVLAATRQKVPVRRYWVRLFVTGFGVIVGFPLFTSLALRELSA
                     ADGAVIVGLLPAATAVMAVLRLGERPSRTFWAASAFGLFAVLLFAASQGAGFLPRAGH
                     LLVLLAVLSGALGYAEGAALSRELGGWRVISWALVFLAPFLVPFVVVAALRSGVSAGP
                     AAWSSFGYLSVVSMYLGFFAWYRGLAMGGVAAVGQIQLAQPILMLLWSALLLGERVGV
                     FTVLCALLVLASVFASQRTRVRKAKT"
     gene            7090..7692
                     /locus_tag="VFJ72_06540"
     CDS             7090..7692
                     /locus_tag="VFJ72_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479157.1"
                     /translation="MREVRIESLEGLEITDGEVRLPADSGRLHIVFPIEVWSSLLASI
                     PTREEPNPEELSELEDIKRHLMEHAAEHSFTTILEGVQCGGERSTTGGGLEYDLVLRP
                     ASLYAATTGYFFTPVIDAEIMARIGASVEEDGFVEQAYIIGAVASEQALAAGDENVRA
                     LSVRGRRCTTSRFEMVPELWQVLSERLGWRNVETFMTEKR"
     gene            7697..9022
                     /locus_tag="VFJ72_06545"
     CDS             7697..9022
                     /locus_tag="VFJ72_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7479158.1"
                     /translation="MPLVDLDRFDFLYSNRVKGMKTSATRDLMATLSRPGIISLAGGF
                     PDTKTFGEAAFREISANIAPDSAQALQYSPTAGLESIKDVIVDVMAAEGTPARQEDVF
                     TTTGAQQGLDLVGKVFLNEGDAILAEGPTYAGALNAFAAYKPRILHIPMDRAGIIPVA
                     ARDGLQKARKQGLHVKFIYTVPNFQNPAGVTMIAERRRELLEIAREFDLIVVEDNPYG
                     MLRFEGEPLPTLAALEQENGGDVDRVVYLGTFSKIFAPGVRLGWVHAQPGILHKINVG
                     KQGADLCSSTLSQMMISSYFQNADWRSYVQRLTALYKERRDTMLDALAEFMPNEVHWT
                     HPEGGLFVWATLPTYLDATAMLPRAIARNVAYVPGEGFYAGSAGKNCMRLNFSFVEPD
                     KIRRGIEILAETIRERMELRGDLERGARSTAGRKSSKADASTSASARRG"
     gene            9026..9985
                     /locus_tag="VFJ72_06550"
     CDS             9026..9985
                     /locus_tag="VFJ72_06550"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET7479159.1"
                     /translation="MARLAVLRGGHSMERDVSFLTGNRVAHALERLGHEVHALDIEET
                     TTERLMEMAPDAAFICLHGPGGEDGTVQALLETLNIPYTGSGPLSSIRCMDKDYAKRA
                     LGAAGIPTPPFRTFLRRAMNEMGAAAALDVAAETLGYPLVVKPAREGSSFGLSRVERP
                     EELLDAVYEAFGYDAKIILEKWVEGAEVSVPILEAPGEEPRILGLVEIRPREGAYNFE
                     AKYTPGMTDFAIPAEVSPEAAARLEASALKTYGVLGCSGFARVDTILDAEGTPWVLDV
                     KTIPGFTETSTLPLAAEAAGMTFEELVETILDAALQSEAPAKL"
     gene            complement(10011..10355)
                     /locus_tag="VFJ72_06555"
     CDS             complement(10011..10355)
                     /locus_tag="VFJ72_06555"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET7479160.1"
                     /translation="MPLYMTQFAYTAETWKALVKKPEDRATVFAKQAEKMGGRMHALY
                     YCMGEYDGLVIFEAPDEQTAAAIVLTVTSPGHLRATRTTPLMTVEEMMEGMHKAKAEA
                     YPTPKLWSPMPS"
     gene            complement(10489..11157)
                     /locus_tag="VFJ72_06560"
     CDS             complement(10489..11157)
                     /locus_tag="VFJ72_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479161.1"
                     /translation="MQERNDIAGPAPLIPAPGPGFASTLLRVAWLAILLGLGMEGLLI
                     LLGTGLGDALGLGQLAADLVRNVSWSVFVCVGLAIGVTVAKARLPVAGLIGFLAAPVA
                     FEVSRVVHRGALEALAATGGVSEGTSPFLIAAIKALEYGALGLAISWLGQRSWAGVMA
                     HALTGLAVGLVFGGSILALTLAAAPQSSTADLISQAVNEVIFPVGCSLILFAANALGK
                     RMAR"
     gene            complement(11254..12579)
                     /gene="trmFO"
                     /locus_tag="VFJ72_06565"
     CDS             complement(11254..12579)
                     /gene="trmFO"
                     /locus_tag="VFJ72_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET7479162.1"
                     /translation="MPDVTVIGGGLAGSEAAWQAAELGCSVELWEMRPVKETPAHRTE
                     HFAELVCSNSLGNRSLETASGLLKEELRRLGSVILRCADENSVPAGGALGVAREDFPR
                     AVTEVVHDHPNIEVVRREAAELPDGPAVIATGPLTSDALVEKIEALSGETLYFYDAAS
                     PIIHRDSIDESVVYRASRYGKGEAAYLNCPMSEEQYYTFVEDLAAAELSPIKDFEEDM
                     YFEGCLPVETIARRGKETLSFGPMKPVGLPDPRTGEIPYAVVQLRQDDAEGRLYNMVG
                     FQTRLKWGEQRRVFRTIPGLENADFARMGVMHRNTYLPANRILEATMRIRKDLSPKPL
                     FFAGQLTGVEGYVESTAMGHIAGTNAARIARGEEPITMPRGTMMGALADYITTKEGTL
                     QPINSNWGLVPAVPKKENGKRLTKPERRQRQARMALDELEQFMGIGSAV"
     gene            complement(12658..13434)
                     /locus_tag="VFJ72_06570"
     CDS             complement(12658..13434)
                     /locus_tag="VFJ72_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7479163.1"
                     /translation="MNTLDLFRLDGRTAIVTGGGRGLGQYMAEALSDAGANVVICSRK
                     IEPLEEVRQEIEARGGKALAVACDVTEPEEVEKVVSAAEETFGSVDIVVNNSGATWGA
                     PAVEMPLEKFDQVMRVNVRGTFLMSQAAGRRMIERGSGGAIINIASVAALVGGDPRYM
                     QTIGYNSSKGAVISMTRDLATSWAPHNINVNAIAPGWFPTRMSGGLIEKFEDRMLEGI
                     PLHRFGNPEDLKGVVVFLASPAASYMTGQTVVVDGGATAW"
     gene            complement(13448..>13723)
                     /locus_tag="VFJ72_06575"
     CDS             complement(13448..>13723)
                     /locus_tag="VFJ72_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479164.1"
                     /translation="EYRGESVKAFVVKRAGAAVTEEEIKAFCAERLAPYKTPKEVEFR
                     DELPKSTVGKLLRRVLADEERAKSGAVASTGASATTGTEAPAPPSPS"
BASE COUNT         2264 a         4638 c         4278 g         2543 t
ORIGIN      
        1 accaccatag ccagcagcgt ggtatccagc atcgtgaacg tggccgccgg agaggtggac
       61 ggcctggcga tgtcgcacgg cgggacccgc cttccgggag acaactctcc gaccgtcggc
      121 gagttcttcg accgcgtgag cggcggcggc agccggaccc gcggtgtctc ggtggaagtc
      181 ggtgagcgcg aagcggccgt ggacctcacc gtgaacgtcg tctacggtaa acccatcgcc
      241 caggtcaccc agtccgtccg tcagaatatt atccgtcgcg tcgagagcct caccggtctc
      301 gatgtcacag aggtaaacat caccgtcaac gacgtgacct tccccgaaca acagtagttt
      361 ttagcgtcaa ctattttggc ttccggtccg accagaagct aaattccaaa ggaacagaag
      421 acgcccccgg cgagaaccgg gggcgtcggc cttacagcta tcgagatacc tctacaccat
      481 tagaagtacc gggttctcga gtgtcttttt gacctcagac atgtagagag cgccgtcgcg
      541 gccgttggcg atgcggtggt cggccgagag ggtgagcttc atgaacgagg cggggacgat
      601 ctcgccgttc tcgtcgtagg acgggcgctt cacgatgctc gaaaccgcga tgatcgaagc
      661 ctgcggcggg ttgatgatgg cggtaaagct atccacgccg aacattccca tgttggagac
      721 cgtgatcgtg cctccctggt actcgtcggg cttgaggccg ccctcgcgcg cccgcgtggc
      781 caggtccttc gactcccgcg atatggtggc gagtcccttg ctctcgatgt tctttatgac
      841 cggcgtgatg agacccgcgt cgagagcgac ggccatcgcc atgttcactg tctcgtgcaa
      901 ctcgatgccc ttgctggtgt agagggcgtt gaggttcggg tagttcctca gagccaccgc
      961 gcacgccttc atcacgaagt cgtttacgga gagcttgatc ccgtcggctt cgagctgggc
     1021 gtttagctgc ttcctcagag ccatcgcggc gtccatctgg acctcgacgg tagcgtagaa
     1081 gtgcgggatg tgctgcttgg cctctgtcat ccggtcgccg atgatccgct gcatcttcgt
     1141 cggctcgacc attgtggtcc ccggcgcccc ggtcggctcc ggcagcttct gggcctggaa
     1201 gcccgcctgc gcggcctgct gcggctgcgc ctcggcggcg ccatcggccg ccgccgcgcc
     1261 ggcttcgccg ccgtcgcgct tcgccgagcc gctctccatc gccgcccgca cgtccctctc
     1321 gacgatgcgt ccggccgggc ccgagccgtc tatcttggag aggtcaaggt cgttctcgtc
     1381 agcgaggcgc cggactatcg gtgaagcgcg gaactggccg ttcgcccgtc catcgccctt
     1441 cccgtcggcc cgggcatctc cgccttccgg aggagcctcg gtctgcgtgg cggtctcggc
     1501 tttggcctcg ccgccctctt cttcggcggc ctcggcggac ccaccgccgc tccgttcggg
     1561 ggcctcctcg ccctcgccct ggataaacgc tatggcctcg ccaacgggca cttcctgacc
     1621 ctcggaggcg ataagttccg ccagggttcc cgcgtcctcg gcttccagct ccatcgtgac
     1681 cttatcggtc tctatctcgg ctatcgcgtc gccttcggag acctcgtcgc cttcgctctt
     1741 gagccacttg aggagggtac cctcctccat cgcgtcgccc atcttgggca tgatcacttc
     1801 gggcatctta cttctcctct atgtacagag cccggcgggc cgcgttcgcc actgctttct
     1861 cactcgggaa ggccgcgagt tccaggttgc gcgcataagg cgccgggacc tccgccccgc
     1921 tcacccgcat caccggagcg tccaggtaat cgaaggcctg ctcctggatg atcgccgcta
     1981 cctcactcgc caccccgaac caccgccatt gctcctgaac cgtcacagcc cggtgagttt
     2041 tcttgaccga ctcgacaatg gtctcctcgt cgaaaggccg tatggaccgg atgtctatga
     2101 cctcggcctg gacgtcgtcc tcctcggcca gcgtgtcggc gacgcgcagg cacaggttca
     2161 cctgccgacc gtaggcgata agcgttacgt ctgagccctc gcgggccacg cgagccttgc
     2221 cgaactccac ggcgttatcg ccttcctcga cctcgccctt cgtaccgtag agggcgccgg
     2281 cttcgaggaa gatcaccggg ttcgggtctc ttatggccgt gatcatcatt cccttcacgt
     2341 cgttcggcgt gacgggcgag acgaccttga ggccggggaa gtgaccgtag aggttctcaa
     2401 gggagtgggt gtgctgcgcg ctaagctgaa cgccgccgcc gttcgggccg cggatgacca
     2461 gcggcacttc gacctgaccg ccggagaaat accgtacctt agcggcgttc tgaatgatct
     2521 ggtccgaggc caggaacgag aagttccagg tcatcatctc gacgaccggc ttcatgccca
     2581 gcatcgccat cccgatggcc gctccggtga acccgccctc ggctatgggg gtgtccatta
     2641 ctctcttggg cccgtactgg tcgaggagac cgtcggtgat gaggtgggtc cctccgtaca
     2701 ccccgatgtc ctcgcccatg aggacgacgg actcatcgcg gtccatctcg tgggtcatgc
     2761 cgtcccgaag ggcctcgcgg taggtcttgg tctccgccat ctcctactcc tcccccatgt
     2821 actcgtcggc tccggcgtag acgtcggtgt agagctcctc tatgggcggc tcgtccgact
     2881 cgtcagcgaa cttgacgacc tcggccacgt actccttggt ctcgctcttt atctcgtcga
     2941 gcttcccttc gtcgaggata tcgttctcga tgagcttgtg ctccagaaga ccgatcgggt
     3001 cgcgctggcg ccactcgttg acctcttcct tggtgcggta tttctccatg aagtccgccg
     3061 ccccgtgcga gacggtccgg taggtcagcg cctccacggc gtagggcttg cccgtctccc
     3121 ggacctgctc cgttaccctc tcggcgcact cgatgacggc ttcgatgtcc atgccgtcta
     3181 ccttctcgtt ctctatgccg taggcgtcga acttggcggc gaggtcggtc atggccgttg
     3241 tccgctcaac cgatgtgccc atgccgtatt tgttgttctc cacgataaag aggcacggac
     3301 acatcccgtc cttaccccag agcccggcga ggttcgccgc ctcgtggaag gcgccgttgc
     3361 tcatggagcc gtccccgagg tagagctggg ttatgtggtc ggtctcctgg tagcgcatgg
     3421 cataggcgaa gccaacacct aagggtatgt gtccggcgac gatgccgtag ccgcccatca
     3481 ggccgcgctc gacgtcgaag aggtgcatac tgccgccctt gcccttgacc atgcccgttc
     3541 ccttgccgta cagctctgcc atgaccgcct tcgggtcggt gccgagtaga agggcgtggg
     3601 cgtggtcgcg gtaggcggtg atgaccttgt cgccttcctt gtaggcttgg aggaatcccg
     3661 tggcgacggc ttcctggcct atatagacgt ggagatagcc gccgatcttg ccctgacgga
     3721 aacccttctg gcatgcttcc tcgaaggctc gtatcttcgc catctcgcgg tatatgtcca
     3781 cgaggcgatc cgtgctggtg gctacggctc cctcactccc ggattcttcg cgagcgccta
     3841 cgtccccgtt ttccgagtgc tcgttatctg ccgacatttc cggccccttc cctctcagag
     3901 gtacaaacct gaaaacagaa taccaaaacc cacggttccg catgtaactt cgtaccctgt
     3961 cccataacga catataccct actccctgcc cgctatcaac cactcgcggg ccgcgcggaa
     4021 ggcccgggcc cgatgagagt cctcattctt ggcctcgccc atctcggcat aggtgagcga
     4081 agagccctcc ggcgcgaaga tagggtccca cccgaaaccg ctctctcctc tcggctccga
     4141 aactacctca cccgccactt ctcccacgaa tacgcggacc tctccatctc catcggcgac
     4201 ggccaccgcg cagacggcgc gcgcccggcg atcctccaca gctccgagca tccgcaagac
     4261 gccctcgtgt cccacgctct ttatgaacca cttggtgagc gcgcccggca gtccgttcca
     4321 ggactccatg atgagcccgg agtcctccac tatgacgggt cgcgagggga aacccagcgc
     4381 ttcgcgagcg gcccgcgcct tcgcggcggc gacttcggcg acgtcgagcg cctgcatctc
     4441 cggcaggtcg aggtcggcgc ggtcgagttc gagacccagt atcgtcgcgg cttcccggcg
     4501 cttgttcggg ttcgaggtaa cgaatacggc tctctggttc cccatgcgcg aaatctacaa
     4561 cacatggagc ctttgtgtca gagtcgcggc catgcatgat ttcagggcgg agagccctaa
     4621 ccgcgcggag tttgagcctc cagcacgcga ttgagccgtt gtactccctc cgcgagcacg
     4681 ccggtctcgg cgccgacgta gcttaaccgc aagaaggatc ccgtcggctc cgccgggaac
     4741 cagcgccgcc ccgcgctgac cataacgccg gccctcatag cgccctccgc gacgagtaca
     4801 tcgtcgagtt catccggcag ccggacccac aggtgcaatc ccccgtcggg cacgagcgtg
     4861 agcctcgcca ctggcaggcc ctctcgaacc gatgcgacca gcgcgtcgcg tctactcttc
     4921 agaaccttgc gcaggtgtcg caggtgacgg ggccacgccg gagacgagag gacctctaag
     4981 gccgcctcct gcaggggacc ggccacgaag aagtcgccga ctattcgcgc ggccttgagc
     5041 ctctccccgg cggcgcccct ggcgcacagg gctcccactc tcatacccgg agcgagcgac
     5101 ttggtgagcg accgcacgta gacgacgtgt ccgtcggggt cgtgggcagc gagcggcgtg
     5161 ggcgggtctc cttcgagggc gaagtcgcgc gcccagtcgt cctcgatcac gaaagcattg
     5221 gcctttcgcg caacctccag gaccgctttc cgtctctcgc ggtcgagcac ggccccgtgc
     5281 gggttggcgt aggtcggctg gcagtagaac agacgcgccc ccgaagaagc gaacgcctcc
     5341 aacaagagat ccagacgcac cccacccgag tccgtcggca cggggaccgg ccttaaacca
     5401 gccgcccgcg cggcggcaat cgcccctata taagtcgggg actccatcag gaccggagaa
     5461 ccgggcgacg cgagcgccct gaaggcgacc gagagcgccg cctgtccccc ggaggtcacc
     5521 acgacatcgg acgccccgaa cgcccctccg acctccctgg caaaccacgc ccgaagctcc
     5581 actacgccct ccaccggaac ccgcccccac gcgccgggac gccgcgccgc ccgcgtcatc
     5641 gccgccgaga gccgcccgag aggctgaagg tcctcgggca gataaccggc agtgagcggc
     5701 aagacccccg gcgcgggcac cgcgagcaac tcgtccagcc cctccgccga gacccccctc
     5761 tcccccaacg ccaccgcctg ccaccccagg tccgccggtt cgaggacctc ccgccgcgga
     5821 gcgacgaagc tgccgcgtcc cggacgcgcc tcaataagcc cttcacccac caaccgcgag
     5881 accgcccgct gcaccgtcgc cggcccggcg ccatgacgcg acatcaactc ccgcacggac
     5941 ggcaaccggt ctccggacgc gccgttctcg gccaacgtcc tcaactcttt agttacgctc
     6001 atcaatgtgt tatcgttatt catggatatt aagagtaacg ttaccggaat aacatcagaa
     6061 acgcaaggtc taatcttcgg cgggctgggg gtgttggctt tcagcttcac ggtcccgctg
     6121 acgcgggtgg ccgtcggcga gttggacgcc acgttcgtgg ggcttgggcg ggcgctcgtg
     6181 gcggccttgc tggcggcggt cgtgctggcg gcgacgcggc agaaagtccc cgtccgccgc
     6241 tattgggtca ggttgttcgt gacggggttc ggggtgatcg tcggtttccc gctgttcacg
     6301 tcgctcgctc tgcgagaact gtccgcggcg gacggggcgg tgatcgtagg tctcttgccg
     6361 gcggctacgg cggtgatggc ggtcttgagg ctcggggagc gcccctcgcg gaccttctgg
     6421 gcggcgtccg ccttcggact cttcgccgtg ctgctgttcg cggcctcgca gggcgcgggg
     6481 ttcttgcccc gcgcggggca tttattggtg ttgctcgccg tactttcggg ggctctgggc
     6541 tatgcggagg gcgcggctct atcacgtgag ttgggcgggt ggcgcgtgat ctcctgggcg
     6601 ctggtctttc tggctccctt cctcgtaccg ttcgtggtgg tcgctgcgct gcgttccggc
     6661 gtctcggcgg gacccgcggc gtggtcgagc ttcggctacc tcagcgtcgt gagcatgtac
     6721 ctgggcttct tcgcgtggta cagggggctg gcgatgggcg gagtagccgc cgtaggacag
     6781 atacagctcg cccagccgat cctcatgctc ttgtggtcgg ccctcttgct cggggagcgc
     6841 gttggtgtat ttacggtcct gtgcgccctg ctcgtcctgg cgagcgtctt cgccagccag
     6901 agaacgcggg tccgtaaagc gaagacctga gatcccgttc ggatcaagat gacaaggagg
     6961 agtaagatcc cggcacccac agacaacacc tcaatacgcg ctgctcgata cgtgaactcc
     7021 gataagacga ctcgcgcatc tctcaccgga gactatttga tcaagacttt ttgtggtagg
     7081 gttggttaca tgcgggaggt cagaatcgag agcctcgaag gactggagat cacggacggc
     7141 gaggtccgcc tgccggccga ttcaggcagg ctccatatcg tgtttccgat cgaggtctgg
     7201 tcgtctctcc tggcaagcat ccccacccgc gaggaaccga atcccgaaga gctgtcggaa
     7261 ctcgaagaca tcaaacggca cctaatggag catgccgcgg agcactcctt caccaccata
     7321 ctagaaggcg tccagtgcgg cggtgagcgc tccaccaccg gagggggact cgaatacgac
     7381 ctggtgttgc gtccggcgag cctctacgcg gcgaccacag gatacttctt cacgcccgtc
     7441 atcgatgcag aaatcatggc tcgcatcggg gcgagcgtgg aggaggacgg cttcgtggag
     7501 caggcttaca tcatcggagc ggtagcttcg gagcaggccc tggccgccgg ggatgagaac
     7561 gtccgggcgc tctccgtgag ggggcggcgc tgcacgacga gccggttcga gatggtaccg
     7621 gagctctggc aggtcctctc cgagaggctg ggctggagga acgtggaaac tttcatgaca
     7681 gagaagaggt agatccttgc cactagtaga cctcgaccga ttcgatttcc tctactcaaa
     7741 ccgggtcaag gggatgaaga cctccgccac ccgcgacctg atggcgaccc tctcccggcc
     7801 cggcatcatc tcgctcgccg gcggcttccc ggacaccaag accttcgggg aggcggcgtt
     7861 ccgcgagatc tccgcgaaca tcgcccccga ctccgcccag gcgctccagt acagtccgac
     7921 cgccggcctg gagtccataa aagacgtcat agtggacgtg atggccgccg agggaacgcc
     7981 cgcccggcag gaggacgtct tcaccaccac cggcgcccag caggggttgg atctcgtcgg
     8041 gaaagtcttc ttgaacgagg gtgacgcgat cctggccgag ggtccgacct acgcgggcgc
     8101 gctcaacgcc ttcgccgcct acaagccgcg catcctgcat atcccgatgg accgcgcggg
     8161 catcatcccg gtcgcggcgc gggacgggct gcagaaggct cgcaagcagg gtctgcacgt
     8221 caagttcatc tacaccgtcc cgaacttcca gaacccggcc ggggtcacca tgatcgccga
     8281 gcggaggcgg gagcttctgg agatagcgcg cgagttcgac ctcatcgtcg tcgaggacaa
     8341 cccgtatggg atgctccgct tcgaaggcga gccgctgccg acgctcgctg cgctggagca
     8401 ggagaacggc ggagatgttg accgggtagt gtacctgggg acgttctcaa agatattcgc
     8461 tccgggagtg cggctcggat gggtccacgc gcagccgggg atactgcaca agatcaacgt
     8521 cggcaagcag ggcgcggacc tctgctcctc cacactctcg cagatgatga tctcctccta
     8581 cttccagaac gcggactggc gctcttacgt gcagcgtctg accgctctct acaaggagcg
     8641 ccgcgacacg atgctcgacg cgctcgcgga gttcatgccg aacgaggtcc actggacgca
     8701 cccggagggc ggcctcttcg tgtgggccac gctcccgacc tacctggacg ccacggcgat
     8761 gctgccgcgg gccatcgcgc gcaacgtcgc ctacgtgccg ggcgagggct tctacgccgg
     8821 gagcgccggc aagaactgca tgcgcctcaa cttctcgttc gtcgagccgg ataagataag
     8881 gcgcggcata gagatactgg ccgagaccat ccgcgagcgt atggagcttc gcggagacct
     8941 cgaacgcggc gcccgctcca ccgccggccg caagagctcg aaggcggacg cttcgacgag
     9001 cgcctccgcc cggagaggct agccggtggc gcgtcttgcg gtactgaggg gcggacactc
     9061 catggagcgg gacgtctcgt tcctgacggg caaccgggta gcgcacgccc tcgaacggct
     9121 cggccacgag gttcacgccc tggacatcga ggagacgacc accgagcgtc ttatggagat
     9181 ggcgccggac gcggccttca tctgcctgca cggccccggc ggcgaggacg gcacggtgca
     9241 ggccctgctc gaaactttga atatccccta caccgggagc gggccgctct cctctatccg
     9301 ctgcatggac aaggactacg caaaacgggc gctgggagcc gccggcatcc cgacgccgcc
     9361 cttccgcacg ttcctgcggc gggcgatgaa cgagatgggc gctgcggcgg ctctggacgt
     9421 ggcggcggag accctcggtt atccgctcgt ggtcaaaccg gcgcgcgagg ggtcaagctt
     9481 cgggctctct cgggtggaga ggccggagga gttgctggat gccgtctacg aggcgttcgg
     9541 ttacgacgcc aagatcattc tggagaagtg ggtcgaggga gcggaggtct ccgtcccgat
     9601 cctcgaagcc cccggcgagg aaccgcgcat cctgggtctc gtggagatac ggccgcgcga
     9661 gggcgcctac aacttcgagg ccaaatacac ccccggcatg accgacttcg cgataccggc
     9721 agaggtctca ccggaagcgg cagcccggct cgaagcttcg gccctcaaga cctacggcgt
     9781 gctcggctgc tccggcttcg cccgcgtgga caccatcctg gacgccgaag gcaccccctg
     9841 ggtcctcgac gtgaagacga tcccaggctt taccgagacc tcgaccctgc cgctcgccgc
     9901 cgaggccgcc ggtatgactt tcgaggagct ggtcgagacc atcctggatg cggcgctcca
     9961 gagcgaggcc ccggcgaagc tttagcggga cctctacagt cccatcgctc ttagctcggc
    10021 ataggcgacc agagcttcgg ggtcggatag gcctcggctt tggccttgtg catcccctcc
    10081 atcatctcct ccacggtcat aagcggtgtc gtcctggtgg ccctgaggtg accgggcgag
    10141 gtgacggtca acacgatagc cgcggcggtc tgctcgtcgg gggcttcgaa gataaccagc
    10201 ccgtcgtact cacccatgca gtagtacagc gcgtgcatgc gtccgcccat cttctcggcc
    10261 tgcttcgcga acacggtcgc ccggtcttcc ggtttcttca ccagagcctt ccacgtttcc
    10321 gccgtgtagg cgaactgcgt catgtagagc ggcatgctcc gacctcctct ccagacatgg
    10381 ccccaccagc catgtgactt agattacaat tatacctcgt cttcgcgact ttgagaaagg
    10441 aatatcgggt actggcgctt ccggtctgga tcccacagat gacgagcgtc agcgcgccat
    10501 cctcttaccc agggcgttgg ccgcgaagag gatcagggag catccgacgg ggaagataac
    10561 ttcgttgact gcctgagata tgaggtccgc agtggaggat tgcggagccg cggcgagcgt
    10621 gagagcgagg atggagcctc cgaacaccag tccgacggcg agtcccgtga gggcatgggc
    10681 catcacgccc gcccacgagc gttgtcccag ccagctaatc gcaagcccga gggctccata
    10741 ctccagcgcc ttgatcgcgg cgatcaggaa cggtgacgtg ccctcggaga ctccgccggt
    10801 ggccgccagc gcctccagcg cgcccctgtg aacgacccgc gagacctcaa acgccaccgg
    10861 cgcggcgagg aacccgataa ggccggcgac cggcaggcgg gctttcgcaa ccgtcacccc
    10921 tatggctaga ccgacgcaca cgaagaccga ccaggagacg ttcctgacga ggtccgcggc
    10981 gagttgcccc aggcccaggg cgtctccgag tccggttccg agcaagatca agagcccctc
    11041 catcccgagg ccgagcagga tcgccagcca cgccacacgg agcagggtgg acgcaaagcc
    11101 cggaccagga gccgggatca acggtgctgg ccccgcgatg tcatttcgtt cttgcataat
    11161 tcagactctc cctggcatga agcggcaggt agagactaaa cgtctcgcag gaagagcgca
    11221 taccgcaaat gaatgatttt cgcgcctcgt cttctagacg gcgctgccaa tacccatgaa
    11281 ctgctccagc tcgtcgagcg ccatccgcgc ctgccgctgt ctccgctcgg gcttggtcag
    11341 ccgcttcccg ttctctttct tcgggacggc gggtacgagg ccccagttgg agtttatcgg
    11401 ctggagcgtg ccttccttcg tggtgatgta gtcggcgagc gcgcccatca tcgtgccgcg
    11461 cggcatcgtt atgggctctt cccctcgcgc tattctggcg gcgttggtcc cggctatgtg
    11521 gcccatcgcg gtgctctcga catagccctc gacgccggtt agttgcccgg cgaagaacag
    11581 gggtttcgga ctgaggtcct tccttatcct cattgtggct tcgagtatcc ggttcgcggg
    11641 gaggtaggtg ttgcggtgca tgacgcccat gcgggcgaag tccgcgtttt cgagaccggg
    11701 gatggttcgg aaaacgcgcc gctgctcgcc ccacttcagg cgcgtctgga aaccgaccat
    11761 gttgtatagc cgcccctcgg cgtcgtcctg cctcagttgc acgacggcgt acggtatctc
    11821 acctgtgcgc gggtcgggca acccgaccgg cttcatcgga ccaaagctca gagtctcctt
    11881 gccccggcgc gcgatggtct cgacgggcaa acaaccctcg aagtacatgt cttcctcgaa
    11941 gtccttgatc ggggacagct ccgccgccgc caggtcctct acaaaagtgt aatactgctc
    12001 ctcgctcatc gggcagttca ggtaagctgc ctcgcccttg ccgtaacggg aggcgcggta
    12061 cacgaccgac tcatctatgg agtcgcggtg gattatgggc gaggccgcgt cgtagaagta
    12121 gagcgtctct cccgagagcg cctcgatctt ctcgaccagc gcgtccgacg taaggggtcc
    12181 agtcgcgatc acggccggtc cgtccggcaa ctccgccgcc tcccgccgca cgacctcgat
    12241 gttcgggtgg tcgtggacca cttccgtcac cgctcgcgga aagtcctcgc gggcgacgcc
    12301 aagagcgcca ccggcgggca cggagttctc atcggcgcag cgcagaatga ccgagccgag
    12361 ccgcctcagc tcctccttca agagccccga agccgtctcc agagagcggt tacccaggga
    12421 gttggagcag accagctcgg cgaagtgctc ggtgcggtgc gcgggggtct ccttcacggg
    12481 ccgcatctcc cacagctcca cggagcatcc aagctccgcg gcctgccagg cggcctcgct
    12541 cccggcaagc ccgccgccta ttacggttac gtctggcata cgaggatctt ccttcgtaag
    12601 aggatcacgt ctaggagccg ctattttacg ccagggacgc cgggtgtcac gcggcggtta
    12661 ccaggccgta gcgccgccat ccacgacgac ggtctgtccg gtcatgtagg aggcggcggg
    12721 cgaggcgagg aagacgacga cgcccttcag gtcttcgggg ttgccgaagc ggtgcagcgg
    12781 gatgccttcg agcatcctgt cctcgaactt ttcgatgagg ccgccgctca tgcgggtggg
    12841 gaaccagccg ggggcgatgg cgttgacgtt aatgttatgc ggcgcccagg aggtcgcgag
    12901 gtcgcgggtc atggagatta cggctccctt cgaggagttg tagccgatgg tctgcatgta
    12961 cctggggtct ccgcccacga gggcggcgac ggacgcgata ttgatgatgg cgccgccgga
    13021 tccacgctcg atcatgcgcc gcccagccgc ctggctcatg aggaaggtgc cgcgcacgtt
    13081 gacccgcatc acctgatcga acttctccag cggcatctcc accgccggag cgccccacgt
    13141 ggcgccggag ttgttgacga ctatatccac gctaccgaac gtctcctcag ccgccgagac
    13201 gaccttctcc acctcctcgg gctcggttac gtcgcacgct accgccaggg ccttgccgcc
    13261 gcgggcttct atctcctgcc gcacctcctc cagcggctct atcttgcggg agcagatcac
    13321 gacgttggcc ccggcgtcgg agagggcctc ggccatgtac tggcccaggc cccggccgcc
    13381 gccggtcacg atggcggtcc tgccatccag tcggaagaga tccagggtat tcaagtccag
    13441 gtcctttcta gctcggagat ggcggagcag gggcttctgt cccggtcgtg gcactcgctc
    13501 ccgtggaagc taccgcgccg gacttcgcgc gctcctcgtc ggccaggacg cggcgcagga
    13561 gcttcccgac cgtgcttttt ggcagctcgt cgcggaactc cacctccttc ggggtcttgt
    13621 acggggcgag gcgctcggcg cagaaagcct tgatctcctc ctccgtgacc gcggctccgg
    13681 ccctcttgac cacgaacgcc ttgacggact cgccccggta ctc
//
LOCUS       DASSMB010000102        12993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1035454, whole genome shotgun sequence.
ACCESSION   DASSMB010000102 DASSMB010000000
VERSION     DASSMB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12993
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035454"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VFJ72_06580"
     CDS             complement(<1..1001)
                     /locus_tag="VFJ72_06580"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7479165.1"
                     /translation="MSRGRFRVIAPAITLMVMVSVLALLLYFPGGLAHHPQASAQASV
                     QTTPKVRTYYIAADEVPWDYAPSGKNEITGEPFDDVANVFVKNGPDRIGHVYRKAQYR
                     EYTDATFEHLKPRPADEEYLGILGPVIRAQVGDTIKVIFKNNTSFPANLHPHGVSYKK
                     DSEGSPYDDGTSGSEKSDDAVAPGKTHTYTWKVPERTGPGPMDPSTILWMYHSHVDEP
                     ADTNSGLIGPMIIGRRGSMNPDGTFKGIDHEVITLFTVFDENSSHYLDYNIQHYAGDP
                     ASVNPDDEEFGESNLMHSINGYVYGNMPMPKMKKGDHARWYTMGMGTEVDLHTPHWHG
                     "
     gene            complement(1842..2630)
                     /gene="budA"
                     /locus_tag="VFJ72_06585"
     CDS             complement(1842..2630)
                     /gene="budA"
                     /locus_tag="VFJ72_06585"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET7479166.1"
                     /translation="MAIDTALVGALHVEYLKERDLGEEAHLHHALFQTSTIDALLHAE
                     YDGDVDFAQLEGRGDFGLGTLDALDGEMIALDGGFYQVKADGRAYEIDGRARTPFAVV
                     TFFEPNLSRTLTAPTDFEGFCTYLDRLVGDVEAPCCAVRLDGHFEYIKTRSVPRQRKP
                     YPPLSEVIKHQPTFELRDVQGSIVGFRFPLYAQGLNVAGYHFHFITDDREAGGHVLGF
                     RLTSGELSVDQEAELRLELPPGVELPAPDLSAAKKEELDRIERE"
     gene            complement(2827..3309)
                     /locus_tag="VFJ72_06590"
     CDS             complement(2827..3309)
                     /locus_tag="VFJ72_06590"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7479167.1"
                     /translation="MSAEENNILEVNKALVRRAYLDGMNSRNMDIIEEVFSTEYLSHF
                     PGQPPTRGIEPIKAVLRSFFDAFPDIVFAVEDQMAEGDKVMTRWTARGTHTGEWRGFP
                     PRDPGIPPTGRHIFIGATDVYLISDGQVVEEWNTLEQLAVLEQIGAVPEPEQILVDGP
                     "
     gene            complement(3350..4621)
                     /locus_tag="VFJ72_06595"
     CDS             complement(3350..4621)
                     /locus_tag="VFJ72_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HET7479168.1"
                     /translation="MNHKDIAQPYFEPDLVADNLEDGYWLEAVDINRDGKPDLVASGL
                     AVGDVVWYENPGWKKHPIAKFPKPVSLDQADVDGDGRLDLVICHNYGSCMYDCQPEDG
                     KISWLKNPGSYENGDNWAVHHIADLMATHRVRCGYFTQRDNLELLALPVVGPEGVHEP
                     IQVTLFSRPADPINSGRWEGRVVNDSSFRIIHGVLVDRLAPGSDLDSVLLASEEGLTW
                     FHHGSDGGWHKSNLASGEQGQVQATGFKGTGNVAIGGIGGDPYAYIATVEPFHGNTVA
                     VYTKETGASLSEVVWKRTVLDVFGDPNEAGEGPGHHVVTADFDGDGDDEFLVALRGPM
                     PWQGVFYYKAIDAQKGLFTKTRVSSSSAARIVVADFDGDGRLDFATTGYYTPGYFLAD
                     NPQILVFLNRFADASAGGNLVEPYATPQVPG"
     gene            complement(4624..5544)
                     /locus_tag="VFJ72_06600"
     CDS             complement(4624..5544)
                     /locus_tag="VFJ72_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HET7479169.1"
                     /translation="MSSRELVTIHASSGLAASIDPHGAQLVSLKTAGGPEIIWQGDPR
                     TWPDHALILFPLISQVPGGHIHHQGCDYPMPPHGFAHSREFDLSYRTGSSCAFELRDD
                     EQTRAQYPFAFRLSIEFDLSDDGLLVTISVENPNGEPMPADVGFHPGFNWPLTPGRLK
                     DEYSIVFDEDEPAPVRRGGDDPVLLYPDGVPTPVEGNVLRPRDELFEGSPIVFDQLAS
                     KSLTYGARGDLSLRIDFPDSPDLALWMIPGANYLCIEPWQGYPALMDFDGPLTEKPGI
                     AVIEPDETRRWRVGLTLQPASEPHVSTGRN"
     gene            complement(5547..6443)
                     /gene="iolE"
                     /locus_tag="VFJ72_06605"
     CDS             complement(5547..6443)
                     /gene="iolE"
                     /locus_tag="VFJ72_06605"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR04379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050114 - myo-inosose-2 dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HET7479170.1"
                     /translation="MFDRDKVSLGITPTGWTNDDMPGVGDDIAFEQCVSEMALAGFEG
                     CSVGHKYPKDSKVLKRELDLRGLRVSEPWASTYFTVAEMEDRTVESFRQQMAFIKEMG
                     GTDVVVAELGQAVHQQPVFVLANKPVFSDEQWGRMVEGLDRLGRMAAEEDMRLCYHHH
                     MGTGVQTRAEVDRLMNDTDPEAVHLLLDTGHLYWAGDDPLDMASAYADRIKHVHLKDI
                     RNEVLDRCMQRNMSFLESMMEGAFTVPGDGVIDFEPIFRVLSDAGYEGWLMVEAEQDP
                     KKANPLEYAKKARSYLREVIGF"
     gene            complement(6448..8124)
                     /locus_tag="VFJ72_06610"
     CDS             complement(6448..8124)
                     /locus_tag="VFJ72_06610"
                     /EC_number="1.1.3.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR02462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyranose oxidase"
                     /protein_id="HET7479171.1"
                     /translation="METNRRHEETENLEVDVLIVGSGPVGSTFARKLVEAGRSVYMVD
                     TGAQHSPRPGEHLKNSFLYQRNVDLFASVIRGHLHNLSVPTNDQPVMTLDPGAYHIDK
                     NKFRGFSLNNQNPDQDPETNLPGAGVTYAVGGMTTHWTCATPRHHPTLERSDIYSDEE
                     WDRLYDEGEKLLNTHKHEFDDSIRHVMVRDTLQEEFSELPDGYGVQNLPLAVERRKDN
                     PKFVHWTGTDTVLGTLADGDQDEEPFILKPQHRCTRLVPSQDGSRIEYAEVQDLMEWK
                     TIHIKANTYIVACNAYLTPQVLYNSGIRPAPLGRYLTEQPMAFCQVVLKQDIVDRILE
                     NPQFAERVKAHREQNPHDPVPIPEDEPEPQAWIPVSEDRPWHCQIHRDAFSYGDVAPN
                     VDTRLVVDLRWFGIVDPNPESRIFFSDKHLDSFGMPQPTFEWILHDEDRRNQHRMMAD
                     MLRAANTLGGFLPGSEPQFVAPGLPLHATGTTRMGSSPDDSVVDENSQVWGIENLYLG
                     GNGLIPRGSASNPTLTSVAMAFRAAEHIIQKEGSPTDETRAAALAFGSGN"
     gene            complement(8181..10232)
                     /locus_tag="VFJ72_06615"
     CDS             complement(8181..10232)
                     /locus_tag="VFJ72_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7479172.1"
                     /translation="MEERVQGTYPPIDDYGIIGAMHTCALVSKAGSIDWFCLPSFDSP
                     AVFARMLDWRKGGHFQVSPKGVQSVSRRYLPGTNVLETTFRTETGVAKLTDFMPASIL
                     EQATSNGHRPRSSSGLSVSLATEDGEAAAETLLKPLEAGLRQKVVRILECTEGSIEFA
                     VECRPRFDYGTVVPHVALVEAGGGGSQYGFARGGGNAISVYCSSVMTISDHGFHSEGA
                     LEAGEKLYSAVTYQPFFVPVCEGFSERGIEQMLDETVRYWEEWSARCDYGGEHKEDIL
                     RSALVLKALTYEPSGALLAAATTSLPEEPAGERNWDYRFTWIRDATFALEALHNLGYT
                     DEARAFKRWLEWTAAYPEDLQIMYGLRGERHLPEFELPLEGYKWSAPVRVGNGAADQF
                     QLDIYGEILDSAYVYRELVVGRAEEADYWEYETETDLDPRQYESDPDYWEFLSAVVEF
                     VIDNWRKPDAGLWESRGGYRHFVYSKVMCWVALDRGIKIAEELVQEKDGPERYGIDDE
                     QLRRWARVRDEIKEDVLANGYDPHYKAGQGAFVQSYGSKTLDASNLRLTEVGFLEADD
                     PRMRSTIEATRRELMSPQGFVYRYKGFDDGLAGAEGTFTICTFWLISNLIALGELHEA
                     RSMFEKLRGYSNDLGLFSEEINEDSGEMLGNFPQAFSHLAFIDVAVELARAERLAVEQ
                     V"
     gene            complement(10380..11180)
                     /locus_tag="VFJ72_06620"
     CDS             complement(10380..11180)
                     /locus_tag="VFJ72_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479173.1"
                     /translation="MQLKALVEGSLLYVGIPAVTLYPLGFIALGVQLWRDPAFPYQNF
                     TTVWEAAALVPETVVMATGIRLIYVSLIAIILGATVTGMLFSLMRRYKKNGEGHEESP
                     PGGIPQSRRLWSIILLLSLPLAATSLLVWGDGPIDGRSDNDLLYIAGFALFALGGGAL
                     IDYVRNRRRKGWFLPGLAAAYAAAILAAMCLSATQTPALSLVRVDVPEGGVGTDNCGR
                     AIRGDTYVKLDEGLSHWYLYNKDGLFAVPHEDLRYVEYEHCPELLDRN"
     gene            12120..12545
                     /locus_tag="VFJ72_06625"
     CDS             12120..12545
                     /locus_tag="VFJ72_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7479174.1"
                     /translation="MLITFKAVSEETGGEYSVFEQTVPPQAGVPPHIHHQESEAFYVL
                     EGEFELLNGGRAMGAGAGEFVLVPRGVVHSFTNLGTMPARFLAIVTPGGLHEKYLSES
                     GQPAETETLPAPPEGPPDVERLRQIASKYHTEMLSPSGQ"
BASE COUNT         2299 a         4061 c         4083 g         2550 t
ORIGIN      
        1 ccgtgccagt gcggggtgtg gaggtcgacc tcggtaccca tgcccatcgt gtaccagcgg
       61 gcgtgatctc ctttcttcat cttcggcatc ggcatgttgc cgtagacgta gccgttgatc
      121 gagtgcatca ggttgctctc cccgaactcc tcatcgtcgg ggttgacgct cgcggggtct
      181 cccgcgtagt gctggatgtt gtagtcgagg tagtggctgc tgttctcgtc gaagacggtg
      241 aagagggtta tcacctcgtg gtctatgccc ttgaacgtcc cgtcggggtt catactgccc
      301 cggcggccga tgatcatggg gccgatcagc ccgctgttgg tgtcggccgg ctcgtcaacg
      361 tgcgagtgat acatccacag tatggtgctg gggtccatcg gtcccggacc ggtgcgctcg
      421 gggaccttcc aggtgtaggt atgcgtcttg cccggagcca cggcatcgtc gctcttttca
      481 gaaccggagg tgccgtcatc gtagggagag ccctcggagt ccttcttata ggacacgcca
      541 tgagggtgca ggtttgcggg gaagctggtg ttgttcttga agatcacctt gatggtgtct
      601 ccgacctgag cccggattac cggccctaaa attcccaggt actcctcgtc tgccgggcga
      661 ggcttgagat gctcgaacgt ggcgtcggtg tactcccggt actgggcctt gcgatagacg
      721 tggccgatcc tgtccggtcc gttcttcacg aacacgttcg ccacgtcgtc gaagggctcg
      781 cccgtgatct cgttcttgcc gctcggcgcg tagtcccagg gcacctcgtc ggcggcgatg
      841 taatacgtcc ggactttagg ggtggtctgg acactggcct gggcactggc ctgcggatga
      901 tgcgccagcc ctccaggaaa gtataggagc agggccagca cactaaccat gaccatcaac
      961 gtgattgccg gagcgatcac cctaaacctg cctctagaca tctgtgtact cctctcacta
     1021 atcgcggaac atgtctacgc cggataatgg ttcaggcgcg ttggacccgc ctcgcaggaa
     1081 tgagttatct tttcttagct caaatgggtt caaccaaatg gttgaaccca tttggttgaa
     1141 acgagctact gcggtgtcga tggcgagcat aggcattggc gggcggtccc gcgcggataa
     1201 acgcgacggt tctctaccgg gtgttccgcg cgcttacaca caaagatcct ggacgcgcgg
     1261 gagatccact atctcctcga ctacctcgaa ggcgaagaag acacaccaca gaggacgagt
     1321 tgaaagcgga gtgaggctcc tcgtgccggc caagacagcg aaaccgggcc ttcaagggcg
     1381 gaacgcggtg cgcttcctca agcgccctat gcgccagata gaaagcaagc tcctcgtgat
     1441 ccgggacggt tctccgatac atcgctcccg gtcagtcaaa atcttttgac caatagcgcg
     1501 gtccgccggc tgcaacttgg acagcttcct ggcgaagctc cggacctcaa ccccgacgag
     1561 ggggtctgga aacatcccaa gtgcgtggag tcgaagaacc ctgccgctag agcctctcgg
     1621 agtcgagagt cgagctatgc aaggccaaag agcgcctcac gcacaaaaga cagctcactc
     1681 tccgttgcat tagacagcca ggattcgagg tttagatgtc cgtgctgaga tcagtacctt
     1741 cgtgactgat agcgcccttc tcgctaacgg cggctggatg gcggcgtaaa acgcggcttc
     1801 agacatcggg gtatttttgg cgctggtcgg ctcgtgccgc gctactctct ctcgatgcgg
     1861 tccagctcct ctttcttcgc ggctgacagg tccggggcgg gcagttcgac gccgggaggc
     1921 agctcgagcc gcagctccgc ctcctggtcg acggagagct cgccgctcgt cagacggaaa
     1981 ccgaggacgt ggccgccggc ttccctgtcg tccgtgatga agtggaagtg gtagccggcc
     2041 acgttgaggc cctgggcgta gagggggaag cggaagccca cgatgctccc ctgtacgtcg
     2101 cgcagctcga aggtgggctg atgctttatg acctcggaga ggggcgggta cggcttgcgc
     2161 tggcggggga cgctgcgcgt cttgatgtac tcgaagtggc cgtccaggcg gacggcgcag
     2221 cacggagcct caacgtcgcc gacgagccgg tcaaggtagg tacagaagcc ctcgaagtcc
     2281 gttggcgcgg tgagcgtccg ggagaggttc ggctcgaaga aggtgacgac ggcgaacggc
     2341 gtgcgcgccc ggccgtcgat ctcgtaggcc cgtccgtccg ccttcacctg gtagaagccg
     2401 ccgtcgaggg cgatcatctc tccgtcgagc gcgtcgagcg tacccagacc gaaatccccg
     2461 cggccttcaa gctgggcgaa gtcgacgtcg ccgtcgtact ctgcgtgaag cagagcgtcg
     2521 atggtcgagg tctggaagag ggcgtggtgg aggtgagcct cctcgccgag gtcccgctcc
     2581 ttgaggtatt cgacgtgcag cgcgccaacg agcgcggtat ctatggccat cggggcatct
     2641 cgtttcaggt tggagtcggg tcgttttggt gggcggatgt cccgctacgt ccgatacgcg
     2701 gcaatacctt atcccgtggt acccatctca tattctaccg ggctatacgg tactccaggg
     2761 ccaaagcggg gtctccgttg gccgaagaac gtccagcccg aactcgcgga caacatccgc
     2821 tgccactcat ggtccatcta ccagaatttg ctctggctcg gggaccgccc cgatctgctc
     2881 cagcacagcc aactgttcga gggtgttcca ttcttccacg acctggccgt cggagatgag
     2941 gtagacgtcg gtagcgccga tgaatatgtg acgacccgtc gggggtattc ccgggtctct
     3001 tggcgggaag ccccgccatt cccccgtgtg cgtaccccgc gcggtccagc gggtcatcac
     3061 cttatcgccc tcggccatct ggtcctcgac cgcaaagacg atgtccggga aggcgtcgaa
     3121 gaaagaccgc agaaccgctt ttatgggctc gatgccgcgc gtcggtggct ggccggggaa
     3181 gtgggagagg tattccgtgg agaaaacctc ctcgataatg tccatgttcc tcgaattcat
     3241 gccatccagg tacgcccggc gcacgagggc tttgttcacc tcgaggatgt tgttctcttc
     3301 ggctgacacc gcgcggtctc ctttcgatcg tgttcagggt agttggtcgt cagcccggaa
     3361 cctgcggcgt agcatagggc tctacgaggt tcccgccggc ggaggcgtcg gcgaaccgat
     3421 tgaggaagac cagtatctgc gggttgtccg ccaggaagta gccgggcgtg tagtacccgg
     3481 tggtggcgaa gtccagccgc ccgtcaccgt cgaagtccgc gacgactatc cttgcggcgg
     3541 aggaagatga gaccctggtc ttggtaaaca gtcccttctg cgcgtcgatg gccttgtagt
     3601 agaagacccc ctgccacggc atcgggccgc gcagcgcgac gaggaactcg tcgtcgccat
     3661 ccccgtcgaa gtcggcagtc accacgtgat gccccggtcc ctcgcccgcc tcgttggggt
     3721 ctccaaagac gtccaggacc gtccgcttcc agacgacttc cgaaagcgac gcgccggtct
     3781 ctttcgtgta gacggcgacc gtgttgccgt ggaatggctc taccgttgcg atgtaggcgt
     3841 agggatcgcc accgatcccg ccaatggcga cgtttccggt acccttgaac ccggtcgcct
     3901 ggacttgccc ctgctctccc gaggccaggt tggacttatg ccagccgccg tcggagccgt
     3961 ggtggaacca ggtaagaccc tcctcgctgg caagcaggac cgagtcgagg tcagagccgg
     4021 gggccaacct gtccacaagg accccgtgga ttatccggaa ggacgagtcg tttaccacgc
     4081 ggccctccca acgcccggag ttgatggggt cagccggcct ggagaaaagc gtcacctgta
     4141 tcggctcgtg aacgccttcc ggccccacga cgggaagcgc cagaagctcc aggttatctc
     4201 tctgcgtgaa gtacccgcag cgaaccctgt gcgtcgccat caggtcggcg atgtggtgta
     4261 cggcccagtt gtcgccgttc tcgtaagagc cggggttctt gagccaggag atcttcccgt
     4321 cctccggctg gcagtcgtac atgcagctgc cgtagttgtg gcagatgacg aggtccaggc
     4381 gcccgtctcc gtcgacgtcc gcctgatcca gggagaccgg cttgggaaac ttcgcgatcg
     4441 ggtgcttctt ccagcccggg ttctcgtacc agacgacatc cccgaccgcc agtccggaag
     4501 ccaccaagtc cggcttgccg tccctgttga tgtctacggc ctccagccag tagccgtcct
     4561 caaggttgtc ggcgaccaga tccggctcga agtacggttg cgcgatgtct ttgtggttca
     4621 tgctcagttc cttcctgtcg agacgtgtgg ttcgctggct ggttgcagag tgaggccaac
     4681 ccgccagcgg cgggtctcgt ccggttcgat cacggctatg cccggtttct cggttagcgg
     4741 gccatcgaag tccatcaggg ccgggtatcc ctgccagggc tcgatgcaga ggtagttcgc
     4801 ccccgggatc atccagaggg ccaggtcggg gctgtcgggg aaatctatcc gcaggctgag
     4861 atcaccgcgc gcgccatagg tcagcgactt gctggcgagc tggtcgaaca cgatgggcga
     4921 cccctcgaac agctcgtcgc gcgggcgcag gacattgccc tcgaccggcg tcggcacgcc
     4981 atccgggtat agaagcaccg gatcatctcc gccgcggcgg accggggccg gctcatcctc
     5041 gtcgaagact atggagtact cgtctttgag ccggccgggg gtcagcggcc agttaaaacc
     5101 gggatggaac cccacgtcgg cgggcatcgg ctcgccgttc ggattctcga ccgagatcgt
     5161 gaccagcaac ccgtcgtccg acagatcgaa ctcgatgctc aaccggaagg cgaacgggta
     5221 ttgggcgcgc gtctgctcgt catcccgcag ctcgaaggcg cagctggatc ccgtcctgta
     5281 ggagaggtcg aactcccggc tgtgggcgaa tccgtgcggc ggcatggggt agtcgcatcc
     5341 ctggtggtga atgtgtccgc cgggcacctg gctgatcagg ggaaacagta tcagggcgtg
     5401 gtcgggccag gtccgcgggt ctccctgcca gatgatctcc gggcctccgg cggtcttcag
     5461 ggagaccagt tgcgccccgt gcgggtcgat ggacgccgcc agaccagatg aggcgtgtat
     5521 tgtgacgagc tctcgcgagg acatgcctag aacccgatca cttcccgcag gtaagagcgg
     5581 gctttcttgg cgtactccag ggggttcgcc tttttcgggt cctgctccgc ctccaccatc
     5641 aaccagccct cgtacccggc gtccgagagc acgcgaaaga tcggctcgaa gtcgataact
     5701 ccatcccctg gtacggtgaa cgctccctcc atcatcgact ccagaaagct catattgcgc
     5761 tgcatgcagc ggtcgagcac ttcgttgcgg atgtccttga ggtggacgtg cttgatgcgg
     5821 tctgcgtacg cgctcgccat gtcgaggggg tcatcgcccg cccagtacag gtggccggta
     5881 tctaggagca ggtgcaccgc ctccgggtcg gtgtcgttca tcaggcggtc cacctcggcc
     5941 cgcgtctgca cgccggtgcc catgtggtgg tggtagcaca ggcgcatgtc ctcctcggcc
     6001 gccatccgcc cgaggcggtc gaggccctcg accatgcgtc cccactgttc gtccgagaag
     6061 accggcttgt tggcgagcac gaagaccggc tgctggtgca ccgcctggcc gagctccgcc
     6121 accaccacgt cggtgccgcc catctccttg atgaaggcca tctgctggcg gaagctctcg
     6181 accgtccggt cctccatctc cgcgaccgtg aagtacgtgc tcgcccacgg ctccgagacc
     6241 ctcaagcctc gaagatccag ctcccgcttt agcactttgg agtccttcgg gtacttgtgg
     6301 ccgacgctgc agccctcgaa tccggccagc gccatctcgc tgacgcactg ctcgaacgcg
     6361 atgtcgtccc ccacgcccgg catgtcatcg ttcgtccagc cggtcggggt aattcccagc
     6421 gagactttgt ctctatcgaa cacgggatca gttccccgag ccgaacgcga gcgccgcggc
     6481 cctcgtctcg tcggtcggcg acccttcctt ctggatgatg tgctccgcgg ccctgaaggc
     6541 catcgcgacg ctggtcaggg tcgggttgct cgccgaaccg cgcgggatga gaccgttgcc
     6601 gcccaggtac aggttctcga tgccccacac ctgcgagttt tcgtcgacca cgctatcgtc
     6661 ggggctactg cccatgcggg tggtgccggt ggcgtgcagc ggcagtccag gcgcgacgaa
     6721 ctgcggctcg gaccccggca ggaagcctcc gagcgtattg gccgcccgca gcatgtccgc
     6781 catcatccga tgctggttcc gtcgatcctc gtcgtgcagt atccactcga aggtcggctg
     6841 cggcatgccg aaggagtcca gatgcttgtc ggagaagaag atgcggctct cgggattggg
     6901 atcaacgatg ccgaaccagc gcaggtcgac gaccaggcgg gtgtccacgt tcggggccac
     6961 gtcgccgtag ctgaaagcgt cgcggtggat ctgacagtgc cacgggcggt cttccgaaac
     7021 tgggatccag gcctgcggct ccggctcgtc ctcggggatg gggaccgggt cgtgcgggtt
     7081 ctgctcccgg tgggctttca cccgttctgc gaactgcggg ttctccagga tccggtcgac
     7141 gatgtcctgc ttcaggacga cctgacagaa agccatcggc tgctcggtca ggtagcgtcc
     7201 gagcggcgcg ggccggatgc cggagttgta gaggacctgc ggcgtgaggt aggcgttgca
     7261 cgcaacgatg taggtattgg ccttgatgtg tatggttttc cactccatca ggtcctgtac
     7321 ctcggcgtac tcgatccgcg agccgtcctg gcttgggacc agccgggtgc agcggtgttg
     7381 cggcttgagg ataaaaggtt cttcgtcctg atcgccgtcg gccagcgtcc ccagcaccgt
     7441 gtcggtgccg gtccaatgca cgaacttggg gttgtccttg cgccgctcga ccgcgagcgg
     7501 aaggttctgc accccgtagc cgtccggcaa ctccgagaac tcttcctgga gcgtatcccg
     7561 taccatcacg tggcggatcg aatcgtcgaa ctcgtgcttg tgggtattga gaagcttctc
     7621 gccctcgtcg tagagccggt cccattcctc atccgagtag atatcgctgc gctccagggt
     7681 gggatgatga cgcggcgtcg cgcacgtcca gtgcgtcgtc atccctccga cggcataagt
     7741 gaccccggcg cccggaaggt tagtctccgg gtcctgatcc gggttctggt tgttgaggga
     7801 gaacccgcgg aacttgttct tgtcgatgtg gtacgcgccc ggatcgaggg tcatgaccgg
     7861 ctggtcattg gtcggtacgg agagattgtg gagatggccg cgaataaccg aggcgaatag
     7921 atctacgtta cgctgataca ggaacgagtt cttgaggtgc tcgccgggac gaggcgaatg
     7981 ctgcgccccc gtgtccacca tgtaaaccga gcggcccgcc tcgacgagct tgcgcgcgaa
     8041 cgtgctgccg acgggtcccg atcctacgat caggacgtcc acctccaggt tctcggtctc
     8101 ttcatgacgt ctattggtct ccatcaaaaa tctcctatca ggtaaatggt ttgtctttgc
     8161 cggccggcgt gtcggaagct ctagacttgc tcgacggcga ggcgttcggc gcgggccagt
     8221 tccacagcga cgtcgatgaa cgccaggtgg gagaatgcct gggggaagtt gcccagcatc
     8281 tcgcccgagt cttcgttgat ctcctcacta aacaggccga ggtcgttaga gtatccccgg
     8341 agcttctcga acatggagcg ggcctcgtga agctcgccca aggcgatcag gttgctaata
     8401 agccagaacg tgcagatcgt gaaggtcccc tcggcgccgg cgagcccgtc atcgaagccc
     8461 ttgtagcggt acacgaaacc ctgcggcgac atcagttccc gccgggtcgc ctcgatggtc
     8521 gagcgcatcc tcgggtcgtc ggcctcaagg aagccgacct ccgtaagcct caggttgctg
     8581 gcgtcgaggg tcttgctgcc gtaggactgg acgaacgcgc cctggccggc cttgtagtgc
     8641 gggtcgtagc cattggccaa tacgtcctcc ttgatctcgt cgcgcaccct cgcccagcgc
     8701 cggagctgct cgtcgtcgat gccgtagcgc tccggcccgt ctttctcctg gacgagttcc
     8761 tcggctatct tgatgccccg gtccagcgcg acccagcaca tgaccttcga gtagacgaag
     8821 tgccggtagc cgccccgcga ctcccacagg ccggcgtcgg gcttgcgcca gttgtcgatc
     8881 acgaactcga ccacggcgct caagaactcc caataatcag ggtccgactc atactggcgg
     8941 gggtcgaggt ccgtctcggt ctcgtactcc cagtagtccg cttcctcagc ccggccgacg
     9001 acaagctccc ggtagacgta cgccgagtcg aggatctcgc cgtagatgtc gagctggaac
     9061 tggtcggcgg cgccgttgcc gacccgcacg ggggcgctcc acttgtatcc ctccagcggc
     9121 agctcgaact cgggtagatg cctctctccg cgcagcccgt acatgatctg caagtcctcg
     9181 gggtacgccg cggtccactc gagccagcgc ttgaacgcgc gcgcctcgtc ggtgtacccg
     9241 aggttgtgca gcgcttcgag cgcgaaggtg gcgtcccgga tccaggtaaa ccggtagtcc
     9301 cagttcctct cccctgcggg ctcctcgggc agggaggtcg tcgccgccgc caggagcgcc
     9361 ccggagggct cgtacgtgag ggccttgagc actagtgcgc tgcgtagaat gtcctctttg
     9421 tgctctccgc cgtagtcgca gcgcgcggac cactcctccc agtaccggac cgtctcatcg
     9481 agcatctgtt cgatcccgcg ctcgctgaac ccctcgcaga cagggacgaa gaagggctgg
     9541 taggttaccg ccgagtacag cttctctccg gcctctagcg cgccctcgga gtggaagccg
     9601 tgatcggaga tggtcatcac cgatgagcag tacacgctga tggcgttgcc gccgccgcgg
     9661 gcgaagccgt actgcgagcc cccgccgccc gcctcgacga gcgcgacgtg gggaacgacg
     9721 gtaccgtagt cgaagcgcgg gcggcactcc accgcgaact cgatgctacc ctcggtgcac
     9781 tccaggatgc ggaccacctt ctggcgcagc ccggcctcca gcggcttgag caacgtctcg
     9841 gcggccgctt ccccatcttc cgtggccaac gagacgctga gaccgctcga ggaccgcggc
     9901 cggtgcccgt tcgaagtcgc ctgttcgagg atagacgcgg gcataaagtc cgtcagcttc
     9961 gcaacgccgg tctccgtccg gaacgtcgtc tccaggacgt tggtccccgg caggtagcgc
    10021 cggctgacgg actggacccc cttcggagag acctgaaagt gaccgccctt gcgccagtcc
    10081 agcatgcgag cgaagaccgc ggggctatcg aagctcggca ggcagaacca gtctatcgag
    10141 cccgccttgg agaccagcgc gcacgtgtgc atcgcgccga tgatcccgta atcgtctatc
    10201 gggggataag tcccctgtac ccgttcttcc atctatctct cctccatcaa agctttcccg
    10261 gtgaacagcc tctgtagctc gtcttgtgta ctcccatggc gcatgctatg tgccgcctaa
    10321 cgcgataggc gaactcattc gggctctgac gagcgttctt tcatggatct tcagaaggat
    10381 cagttccggt ccaaaagctc ggggcagtgc tcgtactcga cgtagcgcag gtcctcgtgg
    10441 ggaacggcga agaggccgtc cttgttgtag aggtaccagt gagagaggcc ttcatccagc
    10501 ttcacgtacg tgtcgccgcg gatcgctctc ccgcagttat cggtcccgac cccgccttct
    10561 ggcacgtcga cccggacgag ggatagggcc ggcgtctgcg tcgctgagag gcacattgcg
    10621 gcgagtatgg ccgcggcgta ggctgccgcc agacccggca ggaaccaacc cttgcggcgc
    10681 cggttcctca cgtaatctat caaggcccct cccccgagcg cgaacagggc gaacccggca
    10741 atgtacagga gatcattgtc ggatcggccg tctatgggcc cgtcgcccca gaccagaagc
    10801 gacgtcgccg ccagcggcag cgagaggagc aatatgatgc tccacaggcg cctgctctgc
    10861 gggattccgc ccggaggact ctcttcatgc ccttcgccgt tctttttgta tcgtcgcatg
    10921 agggagaaga gcatcccggt aacggtcgcg cccaggatta tggcgatcag ggacacgtat
    10981 atcagccgga tcccggtcgc cattaccacc gtctcgggga ccagagccgc cgcttcccag
    11041 accgtggtga agttctgata ggggaaggcc gggtcccgcc acaactggac accgagcgcg
    11101 ataaagccta acgggtagag cgtgacggcc gggataccga catagaggag cgacccttca
    11161 acgagggcct tgagctgcat gccttccggc gccccctcta cgctttcccc tccgtgagta
    11221 tgaggcccgg gcatcggccc gggcttctct ctcacgtcat cgacttaaag cttgcgccaa
    11281 caggcgcagc gtcgatggat tcggagagtg aacaagtatt agaccagcga agccgttgag
    11341 acttcaatac aggcaatttt acgttacagc tcggacagca ggacagcatc cgtccgcagc
    11401 tagggatgct acttacatcg tccaagaaca gcgttattgg ctccccaaag tagtcatgcg
    11461 tgcttatcca catatcatga ccgcattgaa gacaagaagt aggcattggc ccaccttagc
    11521 acgagcttca ccacttggat atacatcttt tgacccaaag cggcgacccg cgcaagtact
    11581 cggctgcgtc ccaaaaggga ccgcaacggc gtcgatgatg ttttcgaggt ccccgcgctc
    11641 aacgcaccga aactggtact gacagcctct gtgcgcccgg ctcactcgtg cgctggacga
    11701 tggtacgtaa tgccgcgacg actcgtagtc gtcactcgaa ttcgcacggc gcaaatagcc
    11761 ttgatgtcgt ggacgcggct ttcttcggcc gcaacggctc tggacataac cttggcagtg
    11821 gtcaggcgga cttgcggacc gtcgcggtag cctaccgcct tcaagaactc cccgcacagg
    11881 tggtaggcgg ttcatgtgct cgatcacggg gttatggtat cgtcaattgt gtctcctccc
    11941 gatccgaagg ccacccaaaa ccttctctgg atcgggagga tattactacc ggcataagaa
    12001 gcagctttag ttgtatagtg cgataaaggg agcgcatatt gttggcaggg gaagctggag
    12061 cggagcaggg tgcctttgga gtgtacccgg acggaggcag gtcgatctgg ttgctgggca
    12121 tgctcatcac gttcaaggct gtgagcgagg aaactggcgg ggagtattca gtcttcgagc
    12181 agacagtgcc gccacaggcc ggggtgccgc cacacataca ccatcaggag tccgaggctt
    12241 tctacgtcct ggagggagag ttcgagcttc tcaatggtgg ccgggccatg ggagcaggcg
    12301 ccggggagtt cgtcctcgtc cccaggggcg tcgtgcacag cttcacgaac ctgggaacga
    12361 tgcccgccag gtttctggcg atcgtcacgc cgggagggtt gcacgagaag tacctatcag
    12421 agtcgggcca accagcggaa accgagacgc taccagcgcc ccccgagggt ccaccggacg
    12481 tcgagaggct caggcagata gccagtaaat accacaccga aatgctgtca ccatcgggtc
    12541 agtaagaaaa cgggaccagt aggctggggc gagcttactc tctacttccc agcctcccgt
    12601 acccaggatc tcaagcttcg gtattcgctt tctcaaacct gtctgaaaac ccgagtgctg
    12661 gatgcgtgtt gcgtgaatta ggagtagaac gaaggccagc cggccactac ccgaccaaag
    12721 gaggcccgga cggcgcagca cgggacctca acgtcgccga cgagccggtc tctgccggag
    12781 gtctctccgg agcgtctgta accagatccg ctttgagatg atcggctatc gcccatccga
    12841 caagcctcct tgagtaggtg tccaaaacga aggccggtta gtcgaagcac ttccaggttg
    12901 gcacatacga gatgtccgcc acccagaggc gatcggaagc ctgtgggctg aattatcagt
    12961 acctggtgtc cgtggaccgc gccgccggct gga
//
LOCUS       DASSMB010000103        15974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1542401, whole genome shotgun sequence.
ACCESSION   DASSMB010000103 DASSMB010000000
VERSION     DASSMB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15974
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542401"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VFJ72_06630"
     CDS             complement(<1..349)
                     /locus_tag="VFJ72_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HET7479175.1"
                     /translation="MVLRLVQVSRPVLWINTIGPAVVGVWLTGELWKWAALPVLLWLT
                     LPFNLLIYGVNDIFDQETDAKNPRKGTLEGARISPSEVRPIRRGVLVSNLPFLLYFLL
                     ALPPSATLWMLLYA"
     gene            399..1166
                     /locus_tag="VFJ72_06635"
     CDS             399..1166
                     /locus_tag="VFJ72_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HET7479176.1"
                     /translation="MKTGNPRPVPLPLAAASALFFCAAAFIVRFPHIPGASVGSYVST
                     FLIALPPCAALFRYLGARRAVVSLIALSAFAYAIETTGVVTGFPYGEFRYGDSLGPKA
                     LGLVPYLLPVSYAPLVLGAVAAAPLRRLVPRVLVSAVLLVVTDAVLDPGAVALGFWTY
                     LNGGSYYGVPASNFAGWLLSGTLAAAILLVLGGGWRSAPPPGLLDGLVIAAAFWSGAA
                     VFSGLPLPALVGFVLFAYLLYRRSRLAGSPAPRPGRV"
     gene            1192..2379
                     /locus_tag="VFJ72_06640"
     CDS             1192..2379
                     /locus_tag="VFJ72_06640"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HET7479177.1"
                     /translation="MKWATATSRRTSMEEAALECAESVARDLGPGSVSLALAFVTPHF
                     AQFYGRIPALLEHYLGPETFLGCSGGGVIGGGEEVERSPALTLVAARLPDVRVTPFHV
                     SGRLPDLDGPPDAWEQLVGARAEDDPQFVLLADPFSARPEALLAGLDYAFPGAAKIGG
                     LVSGGTSPGLNTLFLNGEVFTSGTVGVAMSGDMAVDTVVAQGCRPIGGLMSVTACQGN
                     LLHEVDGRPVFEVLQEVFDGLGEGDRALARTALFVGVLMDEFQEEPGPGDFLIRNLIG
                     IDPHRGAIAVGENLQEGMRLRFHLRDAETSAEDLHAVLTGYEKTIRDPTPVSGALLFS
                     CLGRGEGLYGIPGHDTDIFHEHLGSVPVGGFFCNGEIGPVGGATFLHGYTSSFGLFRP
                     RSA"
     gene            2548..2751
                     /locus_tag="VFJ72_06645"
     CDS             2548..2751
                     /locus_tag="VFJ72_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479178.1"
                     /translation="MIKEQVPQVMISRESRAYQSGWQDGRFGQPGTFAANANLAVWND
                     LDRLAYYRGHRAGRNVRQMLRGN"
     gene            complement(2821..4971)
                     /locus_tag="VFJ72_06650"
     CDS             complement(2821..4971)
                     /locus_tag="VFJ72_06650"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7479179.1"
                     /translation="MKILIAEDDAVSRAIVKRAVERLGHECLAAKDGREAWNVYQSNP
                     DIEVIISDWMMPRMDGLALCNRVRGLEREGYTFFVFLTSLGSREHLLEGMKAGADEYL
                     TKPLDTDQLGVKLIAASRVTSLYRHMQVEDVALRGGSGSRPPGAAMPVTRDGGRGRPK
                     GKVWDILVSRGKISEEQMQKALEAQRGNPAEIGKTLVSLGFISDADLARAQAERLNLE
                     YVELTDSDVDPDAARLVPEKVLRRHMALPLRVEDGRLVVAMSDPTNLYALEDLRMISG
                     RQIRPVVATEESLQRAQTRALSVGERVSEILEEAAQDTVHEEDAVELGAEAGADEAPV
                     VRLVGSVLQRAVGEGASDIHIEPQGQDLKVRLRVDGVLREVMSIPRALQNGVIARLKI
                     LANLDIAERRIPQDGRFSVRLGGRKVDLRVASLPTVYGEEVVLRLLDTTNLQTDLASL
                     GFSPAVLEIYEEVYRRPYGAILVTGPTGSGKSTTLYATLAELNSPERKIITVEDPVEY
                     RMPGVNQVQVNPRAGLTFASGLRSILRSDPDVVMIGEIRDFETAKISVEAALTGHLVL
                     ATLHTNDAPSAINRLTDMGVEPFLTSSAVDCVIAQRLARKLCESCKLPIRIDKEALRQ
                     MGFPFDIGPEDLRFHEALGCDRCGGTGYRGRLGIYEMMVVTEEIKELALRRASTGEIS
                     RAAETNGMVRLRADGLLKAAQGLTTVEEVFRTIV"
     gene            complement(4973..9121)
                     /locus_tag="VFJ72_06655"
     CDS             complement(4973..9121)
                     /locus_tag="VFJ72_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479180.1"
                     /translation="MSVAVATGVYFLLSGTSQTALYNLIGFAMLAALFVGISRHRPAN
                     RLAWNVIAFGLGLYVAGDLVFFNLYTNLLGVPTPFPSVADALYLSSYPVVAFGMALLI
                     RRQGRRDYSAVMDAAIISVGIGLLVWVLLAEPYAGDRSLTVLVRLVSIAYPLLSVLWV
                     ATAARFAFTSAARLPAFRLLIAGVLLHPIADFAYAWLLLNGGYSADHVVNAGWLLSYG
                     LFGAAVLHPSMQDLSTETVGQESTNSGWRIAVLGAAAMMVPVVFVLDTFLGGSHVDLV
                     IAGGSTILLLLILIRMWGLLRTNQKSSAEIRELNESLESRIEERTVQLQRATTESVLN
                     EQKARTLAHYNRTLIDSNLDLLLVLSTDGTINDANLVMEEMTGEPKEALIGTTLDSHM
                     PDAEDATDMLRQALEQGGLRDYEMAVCHHNGHSMPVLFNATTYVNESGETTILATGRD
                     MTYRNLMDEKLRRSEKSFRTVFEQAAIGVVLATPDTRVLLETNPAFQQMLGYTAEELS
                     GKTIAEITAPEDIGADLKINRQLREENLDYYQKEKRYIRKDGRRIWVNLIGSVVRDNE
                     GKAQSIIGMIEDITERREAKEARSQLASIVENSSDAIDSNTLDGTIVSLNPSAQKLYG
                     YSAEEIVGNNISVLTPPDRIDEMKEVLERVGRGEILSEYETVRVSKDGRRIPLSLTVA
                     PVKDQSGNIVGASAIARDITERKQTEEEWRRAREAAEAANRAKSEFLANMSHEIRTPM
                     NGVIGMTGLLLDTDLTEEQREYADTVRTSGENLLTIINDILDFSKIEAGKIDIEIIDF
                     DLRSAVEETVELLAERAHGRELEIASLVEYGVPIALRGDPGRIRQILINLLGNAVKFT
                     EEGEVILRVGLVEETVDAAVVRFEVKDTGIGMTGEQQSRLFQSFTQADSSTTRRYGGT
                     GLGLTISKQLVELMGGAIGIESEPGVGSTFFFTLPLKKQPENALSVREPLTDLGGLRV
                     LVVDDNETNRKIVHHQIISWRMKNGSAEDGQGALEMLRSATGKGSPYDLAVLDMQMPV
                     MDGIELARQIKADPLISSTKLIMLSSMGWRGEGEEVRRAGIDAYLTKPAKQSQLYDAI
                     STVMGMPEGAVALETERQLITRHSLKEAKGRSRGRILVAEDNLVNQKVAVKMLERLGY
                     RADVAANGLETLEALSRIPYEAVLMDVQMPEMDGYEATIGIRQREENEQGRHTPIIAM
                     TANAMQGDREQALEAGMDDYVSKPVNLKKLDEVLERWIRQDSSDSLLTATITSSNGDA
                     SKTEETDATLDAAVIENLRELGGLDLLKELVDLFLDDAPVRISTLRKASREGNAEEFE
                     RTAHTLKGSCGNMGAVRMSEVSARLENIGDSGDLSEVGGLLDRLETELTLARPALIAI
                     SRGS"
     gene            complement(9507..9989)
                     /locus_tag="VFJ72_06660"
     CDS             complement(9507..9989)
                     /locus_tag="VFJ72_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7479181.1"
                     /translation="MEEQTRSEILKTIPYGFYITGVIGEDGEANGFTTCWVSQVSFHP
                     QQVIVAVKKDQHTHDLIESGGVFSLNFLDTLQEDLALKFTEPLQHEDGKVGGQEYTPG
                     EATGAPLFEDAFAHLECRVTGKMEAGDHTVYLGEVAAANLKRPADILTDLDTPMEYGG
                     "
     gene            complement(10052..11629)
                     /locus_tag="VFJ72_06665"
     CDS             complement(10052..11629)
                     /locus_tag="VFJ72_06665"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7479182.1"
                     /translation="MEKVNQGPESPARRVAGIARVGARYGFGFVFGSRLIPHRRRAEP
                     GRVGTRLRRSFEELGPTFVELGRYLASRRDLLPPDVAAELGRPAGAIRPVTFAEVRTL
                     LERELGNTMERLFLSFEEGPARVGVFTQSHRAVLPGERPALVVVNRPGIRRDLLAMRP
                     VADLTRRRLGDRLPLDPSVSVAEFVALAGHRRDMYFAAQTARRLRGLDGFGLRVPEVY
                     RGYSSAKCVTLEAPAKSAVPDTSGYGAVSAALVRLAFEEGIFLADLAPERFVVGDGEV
                     WLADPTETVALDLERLRGVAETLAAVRRGDVDGVTRVLPLAGTFVPRDTTELRRELRE
                     TLGALGGPLWREHSLSEARDRGLEALRKGKTKLNAELAQMLESMVAAERLGGERRTSI
                     VEAAEAAEVVVARYRDPRYVAARTARKLAQPDAYADYPRQIHSFLEELKDGEVEVRFQ
                     HGGLDDLISKVDILANRLVFAVLIAALIIGSSMIGIFSEAGARLLGVSVFGLIGFVFA
                     AILGLLLLIGIIRSGRL"
     gene            11742..12122
                     /locus_tag="VFJ72_06670"
     CDS             11742..12122
                     /locus_tag="VFJ72_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7479183.1"
                     /translation="MQYVHTCYRVFDLDRSIDFYTNKLGLELARKSPIGNDATNAFFA
                     VPGDPEPRLELTLNHDQAEPYELGTGYSHVAFAVEDLDALAEKLEQAGGVDFESKPHA
                     MSSGTRLFFVKDPDGYRIEFIERK"
     gene            12122..12307
                     /locus_tag="VFJ72_06675"
     CDS             12122..12307
                     /locus_tag="VFJ72_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET7479184.1"
                     /translation="MVRAADRGRDLRADLDWLVRRIRDGYEPEKIILFGFLALGEIHA
                     WSDIDLIVVKDTDASSG"
     gene            12443..12841
                     /locus_tag="VFJ72_06680"
                     /pseudo
     CDS             12443..12841
                     /locus_tag="VFJ72_06680"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
     gene            12995..13570
                     /locus_tag="VFJ72_06685"
     CDS             12995..13570
                     /locus_tag="VFJ72_06685"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET7479185.1"
                     /translation="MRGNERGFTLPEVLVTIAILGILIAVAIIIWLGLLEQRRVDAAT
                     NQLVADLRLAHSSASNQLTDWRVVLNPEKADESGDPDYYMVKLAQPYDPDIAGNPSPA
                     VASGTPPRPRHFPANVKIVNINGNLDSGSWIVAPSQADKTRTLEFNSDGTMRFYQAVS
                     GSTCVTVDGDPQNRVVVLAATSRVKVKDGAC"
     gene            complement(13790..14551)
                     /locus_tag="VFJ72_06690"
     CDS             complement(13790..14551)
                     /locus_tag="VFJ72_06690"
                     /inference="COORDINATES: protein motif:HMM:NF026214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET7479186.1"
                     /translation="MATTRAPKRRTYDDGCAAAHALDLVGERWALLVVRELLLGPKRF
                     TDLRAGLPNASPNVLSQRLRELEGAGVVRRRKLPPPAASRIYELTEWGAELEPAVLSL
                     GRWGARSPSKPRDAELSVDSLILSFRTMFDASAAEGFSATYEIRLGEESFRAEVADGR
                     FEISRGVADRPDATIETDPGALAALVYDGRPLAEALRSGDLNLKGDESAVERFLRLFP
                     LPQPARNPPGHRASPPTRARSGALTGAQISNEKLP"
     gene            14679..>15974
                     /locus_tag="VFJ72_06695"
     CDS             14679..>15974
                     /locus_tag="VFJ72_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET7479187.1"
                     /translation="MSSTSERRRWIALYVLCIGMLMIVLDATVVNVALPSIQEDLGFS
                     QSGLAWVVNAYLISFGGLLLLAGRLGDLVSRRGVFLAGIGVFTVASLVCGVARSQEML
                     VAARFVQGVGGAMTSAVILGMIVTMFPGPREQARAIGVFAFVASAGGAVGLLVGGVLT
                     QSISWHWIFFVNVPVGVATAVLARRLIEPDKGIGFGHGADVPGAVLITSALMLGVYTI
                     VEPAADLGWGAGRTLTLGVLSLLLLAAFIAREATARSPLMPLGIFRSRNVAGANVIQV
                     LSVAGMFGMFFLGALYLQRILAYDALETGLAFLPVTIIMGTLSVRYTDRLVMRFGART
                     MVLGGLALFMVGLALFTRAPVGGGYVIHVFPVMVLLGTGAGLCFPALMTLAMSGVTTG
                     DAGLASGLINTTAQVGGALGLAVLATLSASRSSELIRDGQ"
BASE COUNT         2696 a         5202 c         5056 g         3020 t
ORIGIN      
        1 cggcgtagag gagcatccag agcgtcgcgg atggggggag ggctagcagg aagtacagga
       61 ggaacggcag gttcgagacg aggacgccgc gccggatggg acggacctcc gagggggaga
      121 tgcgggcgcc ttcgagggtg cccttgcggg gattcttggc gtcggtctcc tggtcgaata
      181 tgtcgttgac gccgtagatc aggaggttga acggcagcgt gagccagagc agcaccggca
      241 gcgccgccca cttccacagc tccccggtca gccacacccc gaccacggcg gggccgatag
      301 tgttgatcca gagtacggga cgcgagacct gaacgagacg caagaccatg acccaaatat
      361 accagcgtgg caggcgggga ctgagataac atacacgggt gaagaccgga aacccgcgtc
      421 ccgtcccgct accgctcgcc gcggcctccg cgctcttctt ctgcgccgcg gccttcatcg
      481 tccgcttccc gcacatcccc ggcgcgagcg tcggttccta cgtctcgacc ttcctcatcg
      541 ccctgccgcc ctgcgccgcc ctgttccgtt acctcggcgc tcgccgggcg gttgtctccc
      601 tcatcgccct ctcggccttc gcctacgcta tcgagacgac cggcgtcgtc accggcttcc
      661 cctacggcga gttccgttac ggagactcgc tggggccgaa agccctcggg ctcgtgccgt
      721 acctgctccc cgtctcctac gcgccgctag tcctcggggc ggtagccgcc gctcctctgc
      781 gtcggctcgt cccgcgcgtc ctcgtctccg ccgtactgct cgtagtgacg gacgccgtcc
      841 tggaccccgg cgcagtcgcg ctcgggttct ggacctacct gaatggcggt tcctattatg
      901 gtgtacccgc gagcaacttc gccggctggc tgctctctgg gacgctcgcc gcggcaatcc
      961 tcctcgtgct cggcggcggg tggcgctcgg cgccgccgcc aggactgctc gacgggctcg
     1021 tgatagccgc cgcgttctgg agcggagccg ccgtcttctc cggcctgccg ctgccggctc
     1081 ttgtggggtt cgtcctgttc gcgtacctgc tgtaccggcg ttcgcgcctc gccggttctc
     1141 ccgcgccgcg acccggacgg gtatagacta agggaggctc aggaggcaag gatgaagtgg
     1201 gcgacggcca catcgcgcag gacctcgatg gaggaggctg cgctcgagtg cgcggagagt
     1261 gtcgcgcggg acctcggccc cggttcggtc tcgctcgcgc tcgccttcgt cacaccgcac
     1321 ttcgcgcagt tctacgggcg gatacccgcg ctcctggagc attacctggg gcccgagact
     1381 tttctcgggt gttcgggcgg cggcgtcatc ggcgggggag aggaggtgga acgttcgccc
     1441 gctctcaccc tcgtcgcggc ccggctgccg gacgtgcggg ttactccgtt ccacgtctcg
     1501 ggacggctcc cggatcttga cgggccgccg gatgcctggg agcaactcgt gggcgcgcgg
     1561 gcggaggacg acccgcagtt tgtcctgctc gccgacccgt tcagcgcccg gccagaggcg
     1621 ctcctcgccg gactcgacta cgcctttccg ggagcggcca agatcggcgg cctcgtcagc
     1681 ggcggcacct cgcccggcct gaacacgctg ttcctgaacg gcgaggtctt caccagcgga
     1741 acggtgggcg tcgccatgag cggcgacatg gccgtggata ccgtcgtcgc ccagggatgc
     1801 cggcccatcg gcggactgat gagcgttacg gcttgccagg ggaacctgct gcacgaggtg
     1861 gacgggagac ccgtcttcga agtcctgcaa gaggtcttcg acgggctcgg cgagggcgac
     1921 cgcgcgctcg cccgcacggc gctcttcgtc ggggtgctga tggacgagtt ccaggaggag
     1981 ccggggccgg gggacttcct gatccggaac ctcatcggca tagaccctca ccgcggggcc
     2041 atcgccgtcg gcgagaacct ccaggaaggc atgcgtcttc gcttccacct gcgcgacgcg
     2101 gagacctccg ccgaagacct gcacgcggtc ctgaccggct acgagaagac catcagagat
     2161 cctacacccg tctccggcgc gctcctcttc tcgtgcctgg gacgcggcga gggactctac
     2221 ggcatacccg gccacgacac ggacattttc cacgaacacc tcggcagcgt gcccgtcggc
     2281 ggcttcttct gcaacggaga gatcggcccg gtcggcggag cgacctttct tcatggctac
     2341 acttcctcct tcggcctttt ccggccccgc tcggcgtaaa agtactctac gtcccctcgt
     2401 aaataatgtt gctataaggt gtggagatac aaaatatggc ggttttgtaa cttttgttca
     2461 aatttattaa tttccggctt tcattcgcga aagcgatgtg ctataggtgt acacacttgc
     2521 aggaacggcg cgaatcggag gtcggagatg atcaaggagc aggtcccaca ggtgatgatc
     2581 tcgcgggaga gccgggctta ccagagcggg tggcaggacg ggcgcttcgg gcagccgggc
     2641 accttcgcag cgaacgccaa cctcgccgtg tggaacgacc tggaccggct ggcctactac
     2701 cgcgggcatc gcgcgggacg caacgtccgg cagatgctgc ggggcaacta gttctctttt
     2761 cctgatccat taccaggttc aatgcggttg ggcgcgcatg ttcagcgcgc tgttcgccca
     2821 ttacacgatg gtgcggaaga cctcttcgac ggtggtgaga ccctgggccg ctttcagcag
     2881 tccgtcggcg cggaggcgta ccattccgtt cgtctcggcg gcgcgggaga tctcgccggt
     2941 cgaggcccgg cgcagcgcta gctcttttat ctcttcggtg actaccatca tctcgtagat
     3001 gcccaggcga ccccggtagc ccgtcccgcc gcagcggtcg cagcccaaag cttcgtgaaa
     3061 gcgcagatcc tccggcccga tatcgaaggg gaagcccatc tggcggagcg cttccttatc
     3121 tatccgtatc gggagcttgc agctctcgca gagctttctc gcgagacgct gggcgatcac
     3181 gcagtcgacg gcggaagagg tgaggaacgg ctcgaccccc atatccgtca ggcggttgat
     3241 ggccgagggg gcgtcgttgg tgtgcagggt ggcgaggacc aggtggccgg taagggcggc
     3301 ctcgacgctg atcttcgcgg tctcgaagtc ccgaatctca ccgatcatca ccacgtccgg
     3361 gtcgctgcgc aggatgctgc ggagcccgga ggcgaacgtg aggccggcgc gagggttgac
     3421 ctgcacctgg ttcacgccgg gcatccggta ctcgaccggg tcctcgaccg tgatgatctt
     3481 tcgctccggg gagttcagtt ccgcaagggt cgcatacagg gtcgtggact tgccggagcc
     3541 ggtaggtccg gtaacaagga tcgcgccgta cggcctgcgg tagacctcct cgtagatctc
     3601 gagcaccgcc ggtgagaagc cgaggctggc gaggtcggtc tggaggttcg tggtgtcgag
     3661 gagtcgcagc acgacctcct cgccgtagac cgtggggagc gaggccacgc gcaggtccac
     3721 cttcctgccg ccgagccgga cggagaaccg gccgtcctgg gggatgcggc gctcggctat
     3781 gtccaggttg gcgaggatct tgagccgcgc tatcacgccg ttctggagcg cccgggggat
     3841 agacatgacc tcgcgcagca cgccgtccac ccgcagccgg accttcagat cctgcccctg
     3901 cggctctatg tggatgtccg acgcgccttc tccgacggcc cgttgcagga ccgaaccgac
     3961 gagccgcacg acgggggcct cgtccgcgcc cgcttcggcg ccgagctcga ccgcgtcctc
     4021 ttcgtggacc gtgtcctggg ccgcctcttc cagtatctcc gagacccgct ctccgacaga
     4081 tagggcgcgg gtctgggcgc gttgcaggct ctcctcggtg gcaaccaccg ggcggatctg
     4141 gcggcccgag atcatccgca ggtcttccag agcgtacagg ttcgtcgggt cgctcatcgc
     4201 cacgacgagc cgcccgtcct cgacccgcag cggcagcgcc atatgccgac gcagcacctt
     4261 ctccggaacg agccgggcgg catccgggtc cacatcggag tccgtaagct ccacgtactc
     4321 caggttcagc cgctcggcct gggcccgagc gaggtccgcg tcggagataa atcccaaaga
     4381 gacgagggtc ttgccgatct ccgccggatt acctctctgc gcctcaagcg ccttttgcat
     4441 ctgctcctcg gagatcttgc cccgcgacac cagtatgtcc cagaccttgc ccttcggccg
     4501 tccccgtcca ccgtcccgcg taacgggcat ggcggcgccc ggaggtctgg accccgaacc
     4561 tccccgcagg gcgacgtctt cgacctgcat gtggcggtag agcgaggtta cgcgggaggc
     4621 ggcgatgagc ttgactccga gctgatcggt gtcgagcggc ttggtcaggt actcgtccgc
     4681 gccggccttc ataccctcca gcaggtgctc ccggctcccc agggaggtga ggaagacgaa
     4741 gaacgtgtat ccctcgcgct ccagtccgcg cacccggttg cacagtgcca ggccgtccat
     4801 gcgcggcatc atccagtcgc tgatgatcac ctctatgtcc gggttgctct ggtagacgtt
     4861 ccaggcttcc cgtccgtcct tcgccgccag gcactcgtgc cccagccgct ccacggcccg
     4921 cttcactatc gcgcgggaga ccgcgtcgtc ctccgctatc aggatcttca agtcaactcc
     4981 ctcttgagat agcgatgagc gccgggcgtg cgagcgttag ttctgtttcg agccggtcta
     5041 gcagtccgcc gacctccgag agatctccgg agtcgccaat attctccagt cgggcagaga
     5101 cctcgctcat cctgacggcg cccatgttcc cgcagctacc cttgagtgta tgggccgtcc
     5161 gctcaaattc ctcggcgtta ccttctcggg aagctttccg cagggtcgag atccggaccg
     5221 gcgcatcgtc gaggaagaga tcgaccaact ccttcaggag atccaggcct ccaagttctc
     5281 gcaaattctc tatgaccgct gcatccaacg tagcgtcggt ttcttctgtc ttcgaggcgt
     5341 ctccattact cgaagttatg gtggctgtta gcagagaatc cgaggaatcc tggcggatcc
     5401 agcgttccag aacctcgtcg agcttcttta gattcaccgg cttggagacg tagtcgtcca
     5461 tgccagcctc caatgcctgc tcccggtctc cctgcatggc gttcgccgtc atggcgatga
     5521 tcggggtgtg gcgaccttgc tcgttctcct ctcgctgtct tatcccgatg gtggcttcgt
     5581 acccgtccat ctcaggcatc tgcacgtcca tcagaaccgc ctcgtagggt atgcgtgaga
     5641 gggcttccaa ggtttccagg ccgttggccg caacgtccgc ccggtaccca agcctctcca
     5701 gcatctttac ggccactttc tggttcacta aattatcctc ggcgacgagt atccggccgc
     5761 gagagcggcc cttcgcttcc ttaaggctgt ggcgggtgat gagttgtctc tccgtctcga
     5821 gcgccacggc tccctccggc attcccataa ccgttgaaat ggcatcgtag agttgggatt
     5881 gcttggcggg cttggtgagg taggcgtcta tgcccgcccg gcgtacttcc tcgccctcgc
     5941 cacgccatcc catggagcta agcatgatga gcttggtgga agagatcaag gggtcggcct
     6001 ttatctgtcg ggcaagctct atgccatcca tcaccggcat ctgcatgtcc aggaccgcca
     6061 agtcgtatgg actgcctttt cccgtggccg agcgcagcat ctcaagggcg ccctgcccgt
     6121 cctcggcgct accgttcttc atcctccagg agatgatctg atggtgcact atcttgcggt
     6181 tggtctcgtt atcgtccacc acgagtaccc tgagacctcc gaggtcggtg aggggctccc
     6241 ggacggacaa ggcgttttct ggttgttttt ttagaggtag ggtgaagaag aaggtgctcc
     6301 cgactcccgg ctcactctct atccctatcg cgcctcccat gagttccact agctgtttgg
     6361 agatggtaag acctagcccc gtccctccat agcggcgcgt ggtagaggag tcggcctgcg
     6421 tgaaggactg gaagaggcgg ctctgctgct ccccggtcat accgatgccg gtgtccttta
     6481 cctcgaagcg cacaaccgct gcatccacgg tctcttcgac gagcccaacc cttaggatta
     6541 cctctccctc ttctgtaaac ttgacggcgt tgccaagcag gttgatcagg atctgacgta
     6601 tgcgccccgg gtctcccctt agcgcaattg gcaccccata ctccacgagg ctggcaatct
     6661 ccagttctct gccgtgggcg cgctccgcaa gcaactccac cgtctcctcg accgccgagc
     6721 gcaggtcgaa gtctatgatc tcgatgtcta tcttgcccgc ttcgatcttg gagaagtcga
     6781 ggatgtcgtt gatgatggta agcagattct caccagaggt gcgcacggta tcggcgtact
     6841 cccgctgctc ttcggttagg tctgtgtcca gtagcaagcc agtcatgccg atgacgccgt
     6901 tcatgggggt gcgtatctca tgggacatgt ttgccagaaa ctcgcttttg gcgcggttcg
     6961 cggcctcggc ggcctcgcgg gcgcggcgcc attcctcctc ggtctgcttt cgctctgtta
     7021 tgtcgcgggc gatagccgag gctccaacga tgtttccaga ctggtccttt accggggcca
     7081 cggttaaaga gaggggtata cgcctgccgt ctttggagac gcgcacggtc tcgtactcgg
     7141 agaggatctc tcctcgccca accctttcca gcacctcttt catctcatcg atgcgatcag
     7201 gaggcgtaag aacggagatg ttattgccga cgatctcctc cgctgagtag ccgtagagct
     7261 tctgtgcgct cgggttcagg ctcacgatgg tgccgtccag cgtattggag tcgatggcgt
     7321 cggaagagtt ctccacaatg gaggctagct gggaacgggc ctcctttgcc tccctgcgct
     7381 ctgttatgtc ctcgatcatg ccgatgatgg actgcgcttt accctcgttg tcccggacca
     7441 ccgagccgat taggtttacc cagattctcc ggccatcctt acgaatatat cgtttctcct
     7501 tctggtagta atccaggttc tcttcacgca attgacggtt gatcttgagg tccgcaccta
     7561 tgtcttccgg ggccgtaatc tccgcgatgg tcttaccgga aagctcctca gcggtgtaac
     7621 cgagcatctg ctggaacgcg gggttggtct ccagaagcac ccgggtgtct ggggttgcga
     7681 ggactactcc tatggcggcc tgctcgaaga ccgttcggaa actcttctcg cttcggcgta
     7741 gcttctcatc cataaggttg cgataagtca tgtcgcggcc ggtcgccagg atggttgtct
     7801 cgccgctttc gtttacgtag gtggtggcgt tgaacaagac aggcatgcta tgcccgttgt
     7861 ggtggcatac ggccatttcg tagtcccgca gacctccctg ttcgagggcc tgacgcaaca
     7921 tgtccgttgc gtcttccgcg tccggcatgt gtgagtcgag agtcgttccg ataagagctt
     7981 ccttgggctc tccggtcatc tcttccatca ccaggttcgc gtcgttgatc gtgccgtccg
     8041 tgctcaacac caggagtagg tccaggttcg aatcgatcag agtccggttg tagtgggcca
     8101 gcgttctggc tttctgttcg ttgaggacgc tctcggtcgt ggcgcgctgt agctggacgg
     8161 tgcgctcctc gatccggctc tccagggact cattgagctc tcttatctcg gcgctcgact
     8221 tctgattagt gcgcaggaga ccccacatac ggatcaggat gagcaagagc aagatggtcg
     8281 agcctccagc gattaccaga tcgacgtgac ttcccccaag aaatgtgtcc aggacaaaca
     8341 caaccggaac catcatcgcc gcggctccga gcaccgcgat gcgccagccc gagttcgtac
     8401 tctcctggcc cacggtctcc gtagacaaat cctgcatgga cgggtgcagg accgccgccc
     8461 cgaacaaacc gtaggaaagt aaccagccgg cgtttaccac gtggtcggca ctgtagccgc
     8521 cgtttagtag cagccaggcg tatgcaaagt ctgctatagg gtgaaggagc actcccgcga
     8581 tcagcagccg gaaggccgga aggcgcgcgg cggacgtgaa cgcgaaccgc gctgccgtgg
     8641 ctacccacag cacgctaagt aacgggtagg cgatagatac gagtctaacc aacaccgtta
     8701 gagagcggtc accagcgtaa ggctccgcaa gcaacaccca cacgagcagc ccgataccaa
     8761 ccgagatgat cgccgcgtcc atgacagcgc tgtagtcgcg cctcccctga cggcgtatga
     8821 gcagggccat cccaaaggcg acgaccgggt agctgctgag atagagcgcg tcggcgactg
     8881 agggaaacgg tgtcggcacg ccaagcaggt tggtgtagag gttgaagaag accaggtcgc
     8941 ccgctacata cagacccaga ccgaaagcga tgacgttcca ggccaacctg tttgcaggac
     9001 ggtgccgact tatgccgaca aagagcgcag ccagcatcgc gaagccgatg aggttgtaga
     9061 gggcggtctg agaagtccca gacaggagga agtagacacc cgtggcgacg gctacggaca
     9121 ccaggtaaat gatccaggcc cggggaacgg taccagggcc aggtctgccg gttttttgcg
     9181 tcgggctcat cctggatgcg tcgccatcat ataggacatc tgcatcatcg ctccgcgtct
     9241 gtttttgaat catagattac atttagttta tcggacataa gccggaaaga cttaaatgaa
     9301 tccgcgtcgt gaagcatccg ggtacgattt tcagagccat cggtgagcag tccaacctcc
     9361 gctctccatg gagagcggag gttggaccga agtaaagtct ctgaggtctt ttgaggcgag
     9421 gatggtcctg ataccgttgc tggagccccg gaaccacctg ttaaacctgt tgactttcga
     9481 gtggtatggg aggtcgaaag gggagcttac ccaccgtact ccatcggggt gtcgaggtcg
     9541 gtgaggatgt cggcgggacg cttgaggttg gcggctgcga cctcaccgag gtagacggtg
     9601 tggtccccgg cctccatctt tccggtaacg cggcactcca ggtgggcgaa ggcgtcctcg
     9661 aagagcgggg ctccggtcgc ctcgccgggg gtgtactcct gcccgccgac cttgccgtcc
     9721 tcgtgctgta gaggctcggt gaacttgagg gcgaggtctt cctgcaaggt gtccaggaag
     9781 ttcagggaga agacgccgcc ggactcgatc aggtcgtgcg tgtgctggtc tttcttcacg
     9841 gccacgatga cctgctgcgg gtggaacgag acctgcgata cccagcaggt cgtgaacccg
     9901 ttcgcctcgc cgtcctcccc gatcacgccc gtaatgtaga aaccgtaggg gatggtcttc
     9961 aggatctcgc tgcgcgtctg ctcttccatg ctctcccttt ctctctggac tgacaggcgc
    10021 tcttaccccg gagcaccccc gctaaacgat actacagccg cccggagcgg atgattccga
    10081 tgagcagcag cagccccagg atggcggcga agacgaaacc tatcaggccg aagacactca
    10141 cgcccaggag ccgcgcgccg gcctccgaga agatcccgat catggacgac ccgatgatga
    10201 gcgccgcgat gaggacggcg aagacgagcc ggttggcgag tatgtccacc ttggagatga
    10261 ggtcgtcgag gccgccgtgc tggaagcgca cctcgacctc gccgtccttt agctcttcga
    10321 ggaaggagtg gatctggcgc gggtaatccg cgtacgcgtc gggctgcgcg agctttcggg
    10381 cagtgcgcgc cgccacgtag cgcgggtccc tgtaacgcgc caccacgacc tccgcagcct
    10441 ccgccgcctc cacgatgctg gtgcgccgct cgccgccgag cctctccgcc gcgaccatcg
    10501 actccagcat ctgggccagc tcggcgttga gcttcgtttt gcccttccgc agggcctcca
    10561 gcccgcggtc ccgcgcctcg gagagcgagt gctcgcgcca gagagggcca cctaaagcgc
    10621 ccagcgtctc gcgcagctcc cgccgcagtt ccgtggtgtc gcggggcacg aaggtcccgg
    10681 cgaggggcag tacgcgggtc acgccgtcca cgtcgccgcg cctcacggcg gccagggtct
    10741 cggcgacgcc ccgcaggcgc tccagatcca gggccaccgt ctccgtcggg tcggcgagcc
    10801 agacctctcc gtcacccacc acgaaccgct ccggcgcgag gtccgccagg aagatcccct
    10861 cctcgaacgc cagccgcacc agcgccgccg agaccgcgcc atacccggac gtgtccggga
    10921 cagcggactt ggccggagct tcgagcgtga cgcatttggc ggatgagtag ccccggtaca
    10981 cctccggcac ccgcagcccg aacccgtcga gaccccgcag ccgccgcgcc gtctgggccg
    11041 cgaagtacat gtcgcgccgg tgtcccgcca gcgccacgaa ctccgcgacc gagacgctcg
    11101 ggtccagcgg cagacggtcg cccaggcggc gccgcgttag gtcggcgacg ggccgcatcg
    11161 ccagcaggtc gcggcggatg ccggggcggt tcacgaccac gagcgccggg cgctcccccg
    11221 gaagcaccgc ccggtgcgat tgcgtgaaca ctccgacccg cgccggaccc tcctcgaagc
    11281 tcaggaagag ccgctccatc gtgttgccca gctcccgctc caggagcgtc cgcacctccg
    11341 cgaacgtgac cggacggatg gcgcccgccg gacgcccgag ctccgccgcg acgtccggcg
    11401 gcagcaggtc ccggcggctg gcgaggtatc ggccgagctc cacgaaggtc gggccgagct
    11461 cctcgaagga ccggcggagg cgggtcccga cgcggcccgg ctccgcgcgg cggcggtggg
    11521 ggatcaggcg gctcccgaac acgaacccga acccgtagcg ggctccgacg cgcgcgatcc
    11581 cggccacccg ccgggcggga ctctccggtc cctggttcac tttctccatg aggagaagca
    11641 tatcagcgaa acggcccgga gccatcaagg atcagcgaga gcgcaaggca tggcacccgc
    11701 tacaatggta cggacgacag gaaccgacaa gggaggcata cgtgcagtac gtccacacct
    11761 gctatcgggt gttcgacctc gaccgcagca tagacttcta caccaacaag ctcggcctgg
    11821 agctcgcccg caagagcccg atcggtaacg acgccacgaa cgctttcttc gccgtccccg
    11881 gcgaccccga gccgcgcctg gagctcaccc tcaaccacga ccaggcggag ccctacgagc
    11941 tgggcaccgg ctacagccac gtagccttcg ccgtcgaaga cctcgacgcg ctggccgaga
    12001 agctggagca ggccggcggc gtggacttcg agagcaagcc gcacgccatg agcagcggca
    12061 cccgcctctt cttcgtcaag gaccccgacg gctaccgcat agagttcatc gagcggaagt
    12121 agtggtccgg gccgccgaca ggggtcggga cctccgggcg gacctcgact ggctcgtccg
    12181 gcgcatccgg gacgggtacg agccggagaa gatcatcctc ttcggcttcc tcgccctcgg
    12241 cgagatccac gcgtggagcg acatagacct catagtcgtc aaggacaccg acgccagcag
    12301 cgggtgaagg agttgctgcc gctgctgcgg ggccgtttga tcggcgccga cataatcatc
    12361 tatacgcccg aggagtacga acgaggtaaa cgagcccgtt gggggctcgt ccgggatgtt
    12421 gagcgggacg gaaaagttct gtatgaacgc tgctgatgat cccttagcct ggcgaagttt
    12481 agcggacacg gatatggcgg cagctagtgt cctgttttcg gacgaacgag tgggagtgtt
    12541 ggcttccgtc gtttgcttcc atgcgcaaca agcagccgaa aagtatctca agggactgct
    12601 cgtagcgtaa gatcaagaac cgcctcgcat ccacgcgctg acggaactgc tactgcgcgc
    12661 aatcgtgtac gcacccgctt tgaacacttt ggacctagaa aacgctgcta atggactgga
    12721 ccagttctac atccccagcc gttatcccgc cgaggtcggc ggaccagccg gacccattac
    12781 cgccggagaa gccgccgccg tccgtccgcg cctggaggga atactgccgg acccggactg
    12841 atcgaaaccg gcaaacttac cccgccttct ctgtctgacg agtctgcatt gatggacgga
    12901 gagagcgtgc ggcgcactaa agttttcgga gaaaagttcc gaaaatactt tcaaacggac
    12961 gtgacagata ggggtgaaag agaggggtga aagagtgcgt gggaacgagc gcggtttcac
    13021 attgccggag gtcctggtaa ccattgccat actcggcatc ctcatagccg tcgccataat
    13081 catctggctc ggcctgctgg agcagcgccg ggtggacgct gcgacgaacc agctcgtcgc
    13141 cgacctgcgg ctggctcaca gtagcgcatc caaccagctc accgactggc gggtggtgct
    13201 caacccggag aaagcggatg agagcggaga ccccgactac tacatggtga agctggccca
    13261 gccctacgac cccgatatcg cgggaaaccc ttcgcccgcc gtggcttccg gcactccgcc
    13321 gcgtccacgc cacttcccgg caaacgtaaa gatcgtgaac atcaatggca acctggactc
    13381 tggaagttgg atcgtcgcgc cgtcgcaggc cgacaagacc cgcacgctgg agttcaacag
    13441 cgacggtacg atgaggttct atcaggccgt cagcggctcc acctgcgtaa ccgttgatgg
    13501 agatccccag aaccgggtgg tcgtgctggc cgccacctcc cgcgtgaagg tgaaagacgg
    13561 agcctgctga cggtcatcct ggccgacagc agctggatat tagacgagag atattaccta
    13621 cttgagccgt gccccggagt cgaaatccgg tgcgcgcctc cagcccggcc aaaacgcggc
    13681 aaccccctga tgagcggcta ctttcgggag accggccttc ccggaaccag gttcgtccgt
    13741 agctgcggcc actcggggcg acacgcctgc cctgacaggg tgtttcttct catggaagct
    13801 tctcgttgga gatctgcgct cccgtaagcg cgccgctcct ggcccgcgtc ggaggacttg
    13861 ctctatgtcc gggcgggttc cgcgccggtt ggggcagggg gaagaggcgg aggaagcgct
    13921 ccaccgccga ttcgtcgccc ttcaggttga ggtcccccga gcgcaacgcc tctgcgagtg
    13981 ggcggccgtc gtagaccagc gccgccagcg cgccggggtc ggtctcaata gtcgcgtcgg
    14041 gccggtcggc gaccccgcgg gagatctcga agcggccgtc agccacctcg gcgcggaagc
    14101 tctcctctcc cagcctgatc tcgtaggtag cgctgaaacc ctcggcggcg ctcgcgtcga
    14161 acatcgtcct gaacgagagg atgagggaat cgacactcag ctcggcgtcg cggggcttcg
    14221 aaggggagcg ggcgccccag cgtcccagcg agaggaccgc cggctccagc tcagcgcccc
    14281 actcggtcag ctcgtagatc ctcgaagccg ccggcggggg tagcttcctc cgccgcacca
    14341 ccccggcacc ctccaactcg cgcaggcgct gagagaggac attggggctg gcattaggca
    14401 ggcccgccct gagatcggtg aagcgcttcg gccccagcag caactcgcgg actaccagga
    14461 gcgcccagcg ctcgccaacc aggtcgaggg cgtgggcggc ggcgcagcca tcgtcgtagg
    14521 tgcgcctctt cggtgcgcgc gtggtcgcca taaccaggat tctatcaccg gaagttacaa
    14581 gttacattca tttaatctca tttacaaatt catcagttaa attctaggac tatagctgta
    14641 agctcaggtc agactacgaa cccgaggaac ggaggcggat gagttcgacg agtgagagga
    14701 gacgctggat cgccctctac gtgctgtgca tcgggatgct catgatcgtc ctcgacgcga
    14761 ccgtggttaa cgtggcgctg ccgtctatcc aggaggacct cggcttctcg cagtcggggc
    14821 tggcctgggt cgtcaacgcc tacctgatct ccttcggcgg cctgctgctg ctcgccggac
    14881 ggcttggaga cctcgtctcc cgcaggggcg tcttcctggc cggcatcggg gtattcacgg
    14941 tggcgtcgct cgtgtgcggg gtcgcgcgga gccaggagat gctggttgct gcgcgcttcg
    15001 ttcagggcgt gggcggcgcg atgacctcgg ccgtgatcct gggcatgatc gtcacgatgt
    15061 tccccggacc gcgggagcag gccagggcta tcggcgtctt cgccttcgtc gcctccgccg
    15121 gcggcgcggt tggtctgctc gtcggcggcg tcctgaccca gtcgataagc tggcactgga
    15181 tcttcttcgt gaacgtaccc gttggcgtag cgacggcggt gctcgccagg cggctgatcg
    15241 aacccgacaa gggcatcggg ttcggccacg gcgcggatgt cccgggcgcg gtccttatca
    15301 ccagtgctct aatgctcggc gtctacacta tcgtcgagcc ggccgccgac ctcggctggg
    15361 gggccggtcg cactctgacg ctgggcgtcc tctcgctact gttgctcgcc gccttcatcg
    15421 ctcgcgaggc gaccgcgcgc agcccgctga tgccgcttgg gatcttccgc tcgcgcaacg
    15481 tcgcgggggc gaacgtgatc caggtgctga gcgtcgcggg gatgttcggg atgttcttcc
    15541 tcggcgcgct gtatctgcag cgcatcctcg cctacgacgc cctggagacc ggtctcgcgt
    15601 tcctgcccgt cacgatcatc atgggtactc tgtcggtccg ctataccgac cgtctggtca
    15661 tgcgcttcgg cgcgaggacg atggtgctcg gcgggctcgc gctgttcatg gtggggctcg
    15721 ccctgtttac gcgggcgccg gtcggtggcg gctacgtgat ccacgtcttt ccggtaatgg
    15781 tcctgctcgg caccggcgcc ggcctctgct tcccggcgct catgacgctg gcgatgtcgg
    15841 gcgttacgac gggcgacgcg ggtcttgcct ccggcctgat caacacgacg gcgcaggtcg
    15901 gtggcgccct gggattggcc gtgctcgcga ccctgtcggc gtcgcggagt agcgagttga
    15961 tccgggacgg ccag
//
LOCUS       DASSMB010000104        10443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_781599, whole genome shotgun sequence.
ACCESSION   DASSMB010000104 DASSMB010000000
VERSION     DASSMB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781599"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="VFJ72_06700"
     CDS             complement(<1..404)
                     /locus_tag="VFJ72_06700"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7479188.1"
                     /translation="MTDKKVWFITGAGRGMGVDIAKAALASGNAVVATGRRPEAVEQA
                     LGGARDDLLVVKLDITSPADAQAAVRDAVERSGRIDVLVNNAGNFYAGFFEEINPEAF
                     RAQIETTLFGPMNVTRAVLPVMRAQRSGLVVA"
     gene            complement(426..1910)
                     /locus_tag="VFJ72_06705"
     CDS             complement(426..1910)
                     /locus_tag="VFJ72_06705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479189.1"
                     /translation="MRSAMTKEPSAAVTTRRADPGGTLAVAAAGTLLVLVAFTTPLTT
                     LTGTAADLGAGPGAQAWVLSAMNVGTAAGLLSSGAIGDDYGRRRTFFAGALVLAGASV
                     LGALAPNALVLVLGRVLQGLGGAAVIACGLGLIGHAFPSGSARARATGVWGAALGAGV
                     AVGPILAAGLYTIGGWRLPYLLTALAAAALAVAGRALLSESRAAQPRPVDVAGTLLIG
                     LGLAALMAGLVEGRTGWNQLPVIGLLALGLALVAAFVAVERRIAHPMLDLALLGRPDF
                     LGATVGALAAGAGVLALMSLVPTVLERGMGVGAVLAAVVLLAWSATSAVSALGARWLP
                     ARVSPRALLVGGLVGCAAGQLALIGLTPDSSIGRLLPGLLVAGAANGVLNAALGRQAV
                     ASVPADRTAMGSGANNTARYVGSGIGITVVTVLLTRSDAAPGAAGLLSGWNVAVLVTT
                     GFSLLGALIVLLARERPAGPEPDLSGPAGTSGLRRASRGSESIS"
     gene            2147..3085
                     /locus_tag="VFJ72_06710"
     CDS             2147..3085
                     /locus_tag="VFJ72_06710"
                     /inference="COORDINATES: protein motif:HMM:NF018431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET7479190.1"
                     /translation="MNRTQSEREAPRAQASRDELVERIARAVREEGTAEPLEGLRLRR
                     ASSATELGHGVSFPALCVIAQGSKEIWLGDNRYRYDPAHYLITTAALPITTRITEASE
                     ERPYLGVVLRLDPSLVGSVMVEAGHPAPHGHAAVKAIDVSPLDTGLLDAVVRLVRLLD
                     SPAETRFLAPLVKREIVYRLLRGEQGGRLRHTAVLGGHSHLIVRALERLREDFDQPLR
                     IEDIAREFGMSVSGFHHHFKAVTAMSPLQFQKQLRLQEARRLMLGEDLDAASAGYRVG
                     YGDASHFTREYKRLFGAPPARDVERLREASMESASL"
     gene            3169..3582
                     /locus_tag="VFJ72_06715"
     CDS             3169..3582
                     /locus_tag="VFJ72_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7479191.1"
                     /translation="MQIIRNTVETATGPGEWFTGTVYIDALAAPSEPSRLAAASVHFT
                     PGARTAWHTHPFGQTIYVTEGVGLCQRRGGEIEVIRPGDRVFFEPGEDHWHGAAPNRF
                     MTHIAMHEVDDEGSPVTWGEHVSDEEYGAAPPIDQ"
     gene            complement(3639..4913)
                     /locus_tag="VFJ72_06720"
     CDS             complement(3639..4913)
                     /locus_tag="VFJ72_06720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HET7479192.1"
                     /translation="MAYGGINEAWIVGAVRTPIGKHGGSLSPVRPDDLGAVALESLMD
                     RTGVPPSEVEDVYFGCANQAGEDNRDVARMSLLLAGFPETVAGATVNRLCGSGLEAVS
                     NAARAVMLGEADVYVGGGVESMSRAPFVMPKPERAFPTGHTTMYDTTLGWRLVNPKME
                     ALGHTDSLGMTAENLAEERFAVTSEEQEPERLAGDYDISREAQDRFALRSHQKAVAAT
                     DEKRFRDEITPVTVKTRKSETIVEADEGPRRDASLEKMAKLRPAFTKDGSVTAGNSST
                     LNDGSAAVLVVSKEYAEAHGLKPLAKIKSIGVAGVPPRVMGIGPVPATKKALDRAGIT
                     LDDVGLIELNEAFAAQSLAVLYEWGMDPEDERLNPNGGAIALGHPLGASGARILTTLI
                     HEMGRREEVRYGLATMCIGVGQGIAMVVERGN"
     gene            complement(4978..5793)
                     /locus_tag="VFJ72_06725"
     CDS             complement(4978..5793)
                     /locus_tag="VFJ72_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HET7479193.1"
                     /translation="MTDARADASIDKANGKAEGSYTTDEMMTVAASRELRDGVVCFVG
                     IGLPSEAANLARATHAPDCFLVYESGTLGAKPDVLPLSIGDGVLAEHADSVVSVPEVF
                     NYWLQAGRVDVGFLGAAQIDRYGNINTTVVGPYDSPKVRLPGAGGAPEIAASAREVVV
                     ILRQKPRAFVEKLSFVTSVGHREGGDSRKELGYTGGGPTVIITDLGVLRPDPETKEFT
                     LMALHPGATVEQAREATGWDLKVAENVATTEPPTDEELEVLRDLKARTEAARK"
     gene            complement(5790..6647)
                     /locus_tag="VFJ72_06730"
     CDS             complement(5790..6647)
                     /locus_tag="VFJ72_06730"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET7479194.1"
                     /translation="MSEITTLREAVAELVHDGDSVAIEGFTHLIPTAGAHEIIRQERK
                     GLTLVRMTPDLIYDQLIGMGCADKLIFSWGGNPGVGSLHRFRDAVEKGWPRPLEIEEH
                     SHAGMANRYVAGASNLPFAVLRGYVGTDLPKVTKTIAPVTCPFTGERLMAVPALNPDV
                     AIIHAQQADRRGNVAIWGIIGIQKEAALSAKKVIVTVEEVVEAFEPRPFQIVLPSVAV
                     DAISVEPGGAHPSYADGYYDRANAFYGDWDPISRDREKFQSWMERHVLGTEDFAEYMK
                     SIRKEEVVR"
     gene            complement(6772..7176)
                     /gene="pcaC"
                     /locus_tag="VFJ72_06735"
     CDS             complement(6772..7176)
                     /gene="pcaC"
                     /locus_tag="VFJ72_06735"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HET7479195.1"
                     /translation="MSDPLHDRGMKVRREVLGDEHVDRATARTTRFTADFQDLITRYA
                     WGEIWTRPGLDRKTRSCITMTALIALGHLEELKMHVRASLRNGLTEEEIGEVLLQSAI
                     YCGVPAANAAFAVAQSVFAELDGGALETQEDT"
     gene            complement(7173..7961)
                     /gene="pcaD"
                     /locus_tag="VFJ72_06740"
     CDS             complement(7173..7961)
                     /gene="pcaD"
                     /locus_tag="VFJ72_06740"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HET7479196.1"
                     /translation="MTENTVELHHVLEGPEDAPVLVMANSLGTTFRMWDEQAPALSER
                     FRLLRYDHRGHGGSPVPPGPYGMDDLGQDVLALLDRLEIERASFCGLSIGGMVGMWLA
                     SEAPGRIERLVLCCTSARFDPDAYSSRARTVREDGVAKIADAVLERWFTPAFHKSRPD
                     LVEWAGRMLRDTPVEGYAGCCEAISVADLPERIGRIQAPTLVIAGADDPAAPVDKAEF
                     IRDSIPSARLVVVEQAAHLANVEQPETVTRAILDHLAPVAGGGR"
     gene            complement(7958..9316)
                     /gene="pcaB"
                     /locus_tag="VFJ72_06745"
     CDS             complement(7958..9316)
                     /gene="pcaB"
                     /locus_tag="VFJ72_06745"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HET7479197.1"
                     /translation="MSDELFGPIFVPDGFREAVSGRAWLQAMLDAEGALAVAEARAGL
                     IPAQAAEAIVSRCDASLFDPAEIGHEGLAYGNPVPPLVKALTGAVSEVSEDAARHVHK
                     GATSQDITDTAAMLVCRNALGRILAELDALASACARLADTHHDTLMPGRTLLQQALPT
                     TFGLKAAGWLVSVLEARGRLRTVRDSGLAAQLGGAAGTLASLGRDGTQVLREFARELD
                     LAEPVVPWHTGRFRVAEVGSALSLAAGVLGKISLDVILMAQTEVGEVAEPAGDGRGGS
                     STLPHKRNPILSVTAAANVRRVQDLARTLQAAMVQEHERAAGGAWHSEWQALSDALAL
                     TGGAAAAVREVAEGLEVYPERMLENLGATGGLILAEHVTTILSEHLGRLKAHEAVKAA
                     SRRTFESGKSLREELLSESEVREVLSEKEIDAALDPGRYLGSAGEFVDRALDLYRKEV
                     SG"
     gene            complement(9320..9892)
                     /gene="pcaG"
                     /locus_tag="VFJ72_06750"
     CDS             complement(9320..9892)
                     /gene="pcaG"
                     /locus_tag="VFJ72_06750"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET7479198.1"
                     /translation="MAPEITPSQTIGPFFHDALLDRDRSELVPPDHPRAIKVEGTVYD
                     GAGEIVPDAMVEIWQANTSGRYNHPADERDDVPLDEEFSGFGRSGTREGGKFSFVTVK
                     PGPVPGPDGTTQAPHIMVSVFTRGLLKRAVTRIYFPDEEERNAKDPVLQSIEDPELRG
                     TLIARTEGGVLRFDVHLQGEHQTAFFGFGE"
     gene            complement(9892..>10443)
                     /gene="pcaH"
                     /locus_tag="VFJ72_06755"
     CDS             complement(9892..>10443)
                     /gene="pcaH"
                     /locus_tag="VFJ72_06755"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET7479199.1"
                     /translation="DPIGELDSDLTRQHSGEPLGERIIVTGRVLDGDGRPVRNSLIEV
                     WQANAAGRYIHKGDRHPAPLDPNFSGAGRCLTDNDGNYRFVTIKPGAYPWKNHDNAWR
                     PAHIHFSLFGPAFRTRLITQMYFPGDPLFGQDPIFQSVGDPKAQQRMISTFDLETTVP
                     EWALGYKFDIVLEGRNATPRENH"
BASE COUNT         1617 a         3625 c         3456 g         1745 t
ORIGIN      
        1 atcgccacga cgaggccgga gcgctgggcg cgcatgacgg gcagcaccgc gcgggtgacg
       61 ttcatcgggc cgaaaagggt ggtctcgatc tgagcccgga aggcctcagg gttgatctcc
      121 tcgaagaacc cggcgtagaa gttgcccgcg ttgttgacca gcacgtcgat ccggccggac
      181 cgctcgacgg catcccgcac ggccgcctgg gcgtccgcgg ggctggtgat gtccagcttg
      241 acgaccagca gatcgtcccg ggcgccgccc agggcctgct cgacggcctc ggggcgacgg
      301 ccggtggcga cgaccgcgtt gccggaggcc agcgcggcct tggcgatgtc cacgcccatg
      361 ccacggcccg cgccggtaat aaaccaaacc ttcttgtcag tcatgtgttc cacgtccttt
      421 cttgttcatg agatcgattc ggaaccgcga gaggctcgcc gcagtcccga tgtccctgct
      481 ggacctgata ggtcaggctc cggcccggcg gggcgctcgc gggcgagcag cacgatcagc
      541 gcgcccagca gcgagaaccc ggtggtgacc agcaccgcaa cgttccagcc ggagagaagg
      601 ccggccgctc cgggggcggc gtccgagcga gtgagcagca ccgtgacgac ggtgatcccg
      661 ataccggacc cgacgtagcg ggcggtgttg ttggcgccgc tgcccatcgc ggtgcggtcg
      721 gcgggcacgc tggccaccgc ctggcgaccc agggccgcgt tgaggacacc gttggcggcg
      781 ccggccacca gcagaccggg cagcaggcgt ccgatagagc tgtctggggt aaggccgatc
      841 agcgcgagtt ggcccgctgc gcaccccacc agcccgccga ccagcaaggc ccgaggtgac
      901 acccgcgccg gcagccaccg ggcgcccagg gcactcaccg cgctcgtcgc cgaccacgcc
      961 agcagcacga ccgccgccag caccgccccc acgcccatgc cgcgctcgag caccgtcggc
     1021 accaacgaca tcagcgcgag caccccggcc cctgcagcca gcgcgcccac cgtcgccccg
     1081 agaaagtcgg gccggccgag caacgcgagg tcgagcatcg ggtgcgcgat ccgccgctcc
     1141 acggccacga acgcggcgac cagcgccagc ccgagggcca gcagcccgat caccggtagc
     1201 tggttccacc ccgtccggcc ctcgaccagt cccgccatca aggcagcgag tcccagcccg
     1261 atcagcagcg tgccggccac gtcaactggt cgaggctgcg ccgcccgcga ctcggacagc
     1321 agcgcccggc cggctacggc gagcgccgca gcggccagcg ccgtcagcag gtagggcagc
     1381 cgccagccgc cgatggtgta gagcccggcg gccaggatag gtccgaccgc gaccccggcg
     1441 ccaagcgcgg cgccccacac gccggtcgcc cgcgcccgcg ccgaaccaga cggaaaggcg
     1501 tgcccgatca ggccgaggcc gcaggcgatt accgccgccc caccgaggcc ctgcaatacg
     1561 cggccgagga ccaacacgag cgcgttcggg gcgagcgcgc cgagcaccga ggctccggcc
     1621 agcaccagcg ccccggcgaa aaacgtccgg cgccgcccgt aatcgtcgcc gatcgctccg
     1681 ctgctcagca gcccggcggc cgtcccgacg ttcatcgcgc tgagcaccca ggcctgcgcg
     1741 ccgggcccgg cacccagatc ggcggccgtg ccggtcagcg tcgtaagcgg agtggtgaac
     1801 gcgacgagga ccagcagcgt tcccgccgcc gcgacggcga gggtgccgcc cgggtcggcc
     1861 cgccgcgtcg tcaccgcagc ggaaggttct ttggtcatgg cgcttctcat attctttctc
     1921 ttctcgtctc gttgtcagca gaccccgcac ctaccaatgc tggatctctt cttgaacaaa
     1981 agtccaaccg gcttgtccgg atcgagattg acttcctctg tctgacgctg attgtagggt
     2041 catcgcggcg catggtgata taacgattgt cgagaatggt tgtactatcc tgcaaatcgt
     2101 ggagatgggt gttgcgggaa cgagcgaaga ggctgctatg gatttgatga accgtacaca
     2161 gtcagagcgc gaggcgccga gagcgcaagc cagtcgagac gaattggtcg agcgtatcgc
     2221 gcgggccgtc cgcgaagagg ggactgccga gccgctggaa gggcttcggc tccgccgcgc
     2281 atcctcggcc acggagctgg ggcacggcgt ctccttcccc gccttgtgcg taatcgcgca
     2341 gggcagcaag gagatctggc tgggcgacaa tcgctacagg tacgatcccg cgcattacct
     2401 catcaccacc gccgcgctgc ccatcacgac ccggatcacc gaggcgtctg aggagcgacc
     2461 gtatctgggc gtcgttctga ggcttgaccc ctccctcgtc ggctctgtca tggtcgaggc
     2521 cggtcacccc gcgccgcacg gccatgccgc cgtgaaggcc atcgacgtga gcccgctgga
     2581 cacgggcctg ctggacgctg tggtgcggct cgtcaggctt ctggactcac cagccgagac
     2641 gcgcttcctc gcgccgctgg tcaagcggga gatcgtctac cggctcctca ggggagagca
     2701 gggcggcagg ctccgtcaca ccgcggtcct cggcggccac agccatctca tcgtcagggc
     2761 cctggagcgg ctccgcgagg acttcgacca gccgctccgg atcgaggaca tcgcacgcga
     2821 gttcggcatg agcgtctcgg gcttccacca ccacttcaag gcggtcaccg cgatgagccc
     2881 actgcagttc cagaaacaac tgcggctcca ggaggcccgc cgtctcatgc tcggtgagga
     2941 cctcgacgcc gcgagcgccg gctaccgggt gggctacggc gacgcctcgc acttcacccg
     3001 cgagtacaag aggctcttcg gcgcgccgcc ggcgcgcgac gtcgaacggc tgcgggaagc
     3061 ttctatggaa agcgccagcc tataagccga ttagaagccg ctgatcggtc tgcttgactc
     3121 gccgccgccg cgggcaagta aatagtcgaa cgatcttagg aggacgccat gcagatcatc
     3181 aggaacaccg tcgagaccgc gaccgggccg ggcgagtggt tcaccggcac cgtctacata
     3241 gacgcgctcg ccgcgccatc ggagccttcc cgcctcgcgg ccgccagcgt ccacttcacc
     3301 cccggcgctc gcaccgcctg gcacacccac cccttcggac agaccatcta cgtcaccgag
     3361 ggcgtggggc tgtgccagcg ccgcggcgga gagatcgagg taatccggcc aggcgaccgc
     3421 gtgttcttcg agccgggcga ggaccactgg cacggggccg cccccaatcg cttcatgacc
     3481 cacatcgcga tgcacgaggt cgacgatgag ggcagccccg tcacctgggg cgagcacgtc
     3541 agcgacgagg agtacggagc cgcgccaccc atcgaccagt gacgaagctc ctcctcgccg
     3601 gccgtgggct cgcgtacgcc gtcctgcggc tccgagcctc agttccccct ctcgacgacc
     3661 attgcgatgc cctgtccgac acctatgcac atggtggcca gtccgtaacg cacctcttcc
     3721 ctgcgcccca tctcgtggat cagggtggtc aggatacgcg cgcccgaagc tcctagcggg
     3781 tgtccgaggg ctatcgctcc gccgttgggg tttagccgct cgtcttcggg gtccatcccc
     3841 cactcgtaca ggacggccag gctctgcgcg gcgaaggctt cgttcagctc gatcagtccc
     3901 acgtcgtcca gcgtgatgcc ggcacgatcg agcgccttct tcgtggcggg gacgggtcct
     3961 atgcccatga cgcgcggcgg gactccggct acccctatgc tctttatctt cgccaggggc
     4021 ttcagaccgt gggcctcggc gtactccttc gagaccacga gcacggcggc cgagccgtcg
     4081 ttgagggtgc tggagttgcc ggccgtaacg ctgccgtcct tcgtaaaggc gggacggagc
     4141 ttcgccatct tctccaggga ggcgtcgcgg cggggtccct cgtcggcctc cacgatagtc
     4201 tcggacttgc gggtctttac ggttacgggt gtgatctcat cccgaaacct cttctcgtcc
     4261 gtcgcggcca cagccttctg gtggctgcgg agggcgaagc ggtcctgggc ctcgcgagag
     4321 atgtcgtagt cgccggcgag cctctcgggc tcctgctctt cggaggtaac ggcgaaacgc
     4381 tcctcggcca ggttctcggc ggtcatgccc agggagtcgg tgtggcccaa cgcctccatc
     4441 ttcgggttga ccaggcgcca acccagggtg gtatcgtaca tggtcgtgtg gcccgtggga
     4501 aaggcccgct cgggcttggg catcacgaac ggagcccggc tcatggactc gacgcctccg
     4561 cccacgtaca cgtccgcctc gccgagcatg acggcccgcg cggcgttaga gacggcttcc
     4621 aggccggagc cacacagcct gttcaccgtc gctccggcaa ccgtctcggg gaagccggcg
     4681 aggagtagtg acatcctcgc cacgtcgcgg ttatcctcgc cggcctggtt ggcgcagccg
     4741 aagtagacgt cctcgacttc cgagggtggt actccggtgc ggtccatcag agattccagg
     4801 gctaccgccc cgaggtcatc tgggcggacg gggctgaggg agccgccgtg cttgcctatg
     4861 ggcgtgcgta ccgcgcccac gatccaggct tcgtttattc ctccgtaagc catacttcag
     4921 ttctcctttc agccgagggt tggatccagc cctcgccatc gaacgtcttc ttcccgctca
     4981 cttcctcgcc gcctcggtcc tggccttcag gtcgcgcagg acctctagct cttcatccgt
     5041 cggcggctcg gtggtcgcta cgttctccgc caccttcagg tcccagcccg tcgcttcacg
     5101 cgcctgctcg acggtcgcgc cggggtgcag agccatgagg gtgaactctt tagtctcggg
     5161 atcgggcctg aggacgccga ggtcggtgat gatcacggtg ggaccgccgc ccgtatagcc
     5221 caactctttg cgcgagtcgc cgccctcgcg gtgtccgacc gaggtcacga aggagagctt
     5281 ctcgacgaag gcgcggggct tctgacgcag gatcacgacg acctccctgg cagacgccgc
     5341 tatctccggc gctcctcccg ctccggggag gcgcaccttc ggggagtcgt agggaccgac
     5401 gacggtggtg ttgatgttcc cgtagcggtc tatctgcgcc gcgcccagga agccgacgtc
     5461 cacgcgcccg gcctgcagcc agtagttgaa tacctccggc accgagacga ccgagtcggc
     5521 gtgctcggcc aggacaccgt cgccgatgct gagtggcagc acgtccggct tggcgcctaa
     5581 agttcccgac tcgtatacca ggaagcagtc cggcgcgtgc gtggcgcggg ccaggttcgc
     5641 cgcttcgctc ggaaggccga tgccgacgaa gcacaccacc ccgtccctca gctcgcggga
     5701 cgcggccacg gtcatcatct cgtcggtggt gtatgagcct tcggctttgc cgttggcctt
     5761 gtctatgctt gcgtctgctc ttgcgtccgt caccggacca cctcctcttt gcgaatgctc
     5821 ttcatatact cggcgaagtc ttccgtcccg aggacgtggc gctccatcca ggactgaaac
     5881 ttctcccggt cgcggctgat cgggtcccag tcgccgtaga aggcgttggc gcggtcgtag
     5941 tagccgtcgg cgtaggacgg gtgcgcgccg cccggctcta ccgagatagc gtccacggca
     6001 acggagggca gcacgatctg gaacggccgg ggctcgaacg cctcgaccac ttcctcgacc
     6061 gtgacgatga ccttcttcgc cgagagcgcg gcctccttct ggatgccgat gatgccccag
     6121 atagcaacgt ttcccctccg gtcggcctgc tgggcgtgga ttatcgccac gtccgggttg
     6181 agcgcgggca cggccattag tctctcgccg gtgaacgggc acgttaccgg ggctatggtc
     6241 ttcgtcactt tgggcagatc ggtgccgaca tagccgcgca gcacggcgaa ggggaggttg
     6301 gaagctccgg cgacgtagcg gttcgccatc ccggcgtggg agtgctcctc tatctctaag
     6361 ggccggggcc agcccttctc gacggcgtcg cggaaacggt gcagggagcc cacgccgggg
     6421 ttgccgcccc aggagaagat gagcttgtcg gcgcacccca tcccgatcag ttggtcgtag
     6481 atcaggtccg gcgtcatgcg gacgagcgtc agacctttgc gctcctggcg gatgatctcg
     6541 tgcgcccccg cggtcgggat gaggtgcgtg aaaccctcga tggctaccga gtcgccgtca
     6601 tgcaccagct ccgcgaccgc ctcgcgcagc gtcgttatct cactcaactt aggttctccc
     6661 ttccgagagc gagatagcct cgcggcgtta tcccggctcc tgttcgttgt acgggatcag
     6721 cgggtcttac gtcactccgc cagccgcatc tacacttcat tccgcgctcc ctcatgtatc
     6781 ctcctgtgtt tccagtgccc cgccatcgag ttcagcaaag acgctctgag ctacggcgaa
     6841 ggcggcgttg gcggccggca caccgcagta gatagcactc tggagcagga cttcgcctat
     6901 ctcttcttcc gtgagcccgt tacgcagcga tgcccggacg tgcatcttca gctcttccag
     6961 gtgtcccagc gcgatgaggg cggtcatggt gatgcagctt cgcgtcttgc ggtccaggcc
     7021 ggggcgcgtc cagatctcac cccaggcgta gcgggtgatg agatcctgga agtcggcggt
     7081 gaacctggtg gtgcgcgccg tcgcccggtc aacgtgctcg tcgcccagga cctcgcggcg
     7141 caccttcatg cctcggtcgt ggagaggatc gctcatcttc ctcctccggc taccggagcg
     7201 agatggtcca ggatcgcccg cgtcacggtt tcgggttgct ccacgttcgc caggtgcgcc
     7261 gcctgctcca cgactacgag acgggcgctg gggatcgagt cgcggatgaa ctcggccttg
     7321 tccaccggcg cggccgggtc gtcagccccg gcgatcacga gcgtgggagc ctgtatcctt
     7381 ccgatcctct ccggcaagtc tgctaccgag attgcctcgc agcatcccgc gtatccctcc
     7441 accggggtgt cgcgcagcat gcgcccggcc cactcgacga ggtccgggcg gctcttgtga
     7501 aacgctggcg tgaaccagcg ctccagaaca gcgtcggcga tcttcgcgac accgtcttcc
     7561 cggaccgtcc gggcacgaga gctgtacgcg tcgggatcga agcgggccga ggtgcagcac
     7621 agcaccagac gttcgatacg cccgggagcc tcactcgcca gccacatccc aaccattcct
     7681 ccgatagata agccgcagaa cgaagcgcgt tcgatctcca gccggtccag gagcgccagt
     7741 acgtcttgac cgaggtcgtc catcccgtaa ggaccgggcg gaacagggga acctccgtgt
     7801 ccgcggtggt cgtaacgcaa caggcggaag cgttcgctca aggcgggagc ctgctcgtcc
     7861 cacatacgga acgtcgtgcc cagagagttc gccatgacca gcacgggcgc gtcctcggga
     7921 ccttccagga catggtgcag ctcgacggtg ttctccgtca tccggatacc tccttgcggt
     7981 acaggtctaa tgcccggtcc acaaactccc cggccgatcc caggtagcgc cctggatcga
     8041 gcgcggcgtc gatctctttc tcggagagga cttccctgac ttcggactcg gagagaagct
     8101 cctcgcgcag cgacttcccg ctctcgaacg tccgccggga ggcggctttt acggcctcgt
     8161 gggccttcag ccgcccgaga tgctccgaga ggatcgtcgt gacgtgctcg gcgaggatca
     8221 aacctcccgt cgcaccgagg ttctccaaca tcctctccgg gtagacttcc agaccctccg
     8281 ccacctcgcg caccgccgcc gccgctccgc cggtgagggc gagcgcgtcg gagagggcct
     8341 gccactcgga atgccaggct ccgcccgcgg cccgctcgtg ctcctgaacc atcgccgctt
     8401 gcagcgtgcg cgcgaggtcc tggacgcgcc tgacgttcgc ggcggcggtt acggagagga
     8461 tggggttgcg cttgtggggc agggtcgagg agccgccgcg accatcgccc gcgggttcgg
     8521 cgacctcgcc gacctcggtc tgggccatca ggattacgtc gagagatatc ttcccgagga
     8581 cgcccgcagc gagcgagagc gcgctcccga cctccgcgac ccggaaccgt ccggtgtgcc
     8641 aggggacgac gggctcggcg aggtccaact cccgcgcgaa ctcccgcaat acttgtgttc
     8701 cgtcccgccc gagggaggcg agcgtcccgg cggcgccccc gagctgcgcg gccagccccg
     8761 agtcacgcac ggtacgcagc ctaccgcgcg cctccagtac ggatacgagc cagcctgcgg
     8821 ccttgaggcc gaaggtcgtg gggagggcct gctggagcag cgtgcggccg ggcatcaggg
     8881 tatcgtggtg ggtatccgcg agccgggcgc aggccgatgc gagagcgtcc agctcggcca
     8941 ggatccggcc gagcgcgttc cggcagacga gcatcgcggc ggtgtccgtg atgtcctggc
     9001 tggtcgcgcc cttgtggacg tggcgggcgg cgtcttccga cacttcggag acggctccgg
     9061 tgagcgcctt gacgagcggc gggacggggt tgccgtaggc gagtccctcg tggccgatct
     9121 ccgccgggtc gaagaggctg gcgtcgcagc gggagacgat ggcctcagcc gcctgggccg
     9181 ggatcaggcc ggctcgcgcc tccgcgacgg ccagggcccc ttccgcgtcg agcatggcct
     9241 gcagccatgc cctccccgag acagcttcgc ggaacccgtc cgggacgaag atcggcccga
     9301 atagctcatc ggacatccgt cattccccga acccgaagaa cgcggtctgg tgctcgccct
     9361 gaagatgcac gtcgaagcgc agcacgcctc cctcggtgcg ggcgatcaac gtcccgcgaa
     9421 gctccgggtc ttcgatggac tggaggacgg ggtccttagc gttccgctcc tcctcgtcgg
     9481 ggaagtagat gcgggtcacg gcgcgcttga ggaggccgcg ggtgaagacg gagaccatta
     9541 tgtgcggggc ctgggtcgtc ccgtccgggc cgggcacggg gccgggcttg acggtgacga
     9601 aggagaactt tccgccttcc ctggtcccgg agcggccgaa gccggagaac tcctcgtcca
     9661 gggggacgtc gtcccgctca tcggcgggat ggttgtaacg acccgaggtg ttggcctgcc
     9721 agatctcgac catcgcgtcc ggcactatct cgcccgcgcc gtcgtagacg gttccctcga
     9781 cctttatggc cctcggatgg tccggcggga ccaactcgga gcggtcccgg tcgaggagcg
     9841 catcgtggaa aaaggggcct atcgtctggg agggtgtgat ctcgggggcc atcagtggtt
     9901 ctccctcggg gtcgcgttgc gtccttcgag cacgatgtcg aacttgtacc cgagcgccca
     9961 ctccggcacg gtggtttcga ggtcgaaggt cgagatcatg cgctgctgcg ccttcgggtc
    10021 gcccacggac tggaagatgg ggtcctgccc gaagagaggg tctccgggga agtacatctg
    10081 ggtgatgagg cgcgtcctga acgccgggcc gaagagcgag aagtgtatgt gcgccggccg
    10141 ccaggcgttg tcgtggttct tccacgggta cgcgcccggc ttgatcgtga cgaagcggta
    10201 attaccgtcg ttgtcggtga ggcacctacc cgcgccggag aagttcgggt cgagcggcgc
    10261 cggatgccgg tcgcccttgt ggatgtatcg cccggcggcg ttcgcctgcc agacctcgat
    10321 gaggctgttc ctcaccgggc gcccgtcacc gtcgagcacg cgccccgtga cgatgatcct
    10381 ctctcccagc ggctcccccg agtgctggcg cgtcaggtcg ctgtcaagct cccctatcgg
    10441 gtc
//
LOCUS       DASSMB010000105         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_528055, whole genome shotgun sequence.
ACCESSION   DASSMB010000105 DASSMB010000000
VERSION     DASSMB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528055"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..228)
                     /locus_tag="VFJ72_06760"
     CDS             complement(25..228)
                     /locus_tag="VFJ72_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479200.1"
                     /translation="MEPVEPTPETLRKIVAGPVGEEEERRLLACPWCDSGDVEMVSEY
                     GPHLMVYSYICRECHSPFEVIKR"
     gene            complement(183..998)
                     /locus_tag="VFJ72_06765"
     CDS             complement(183..998)
                     /locus_tag="VFJ72_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET7479201.1"
                     /translation="MSERPEVTEEVAPSRGAGSEFLVDDDHALAALVNLIAVIGDNEY
                     FLGRRVSEWADAGPLLESTAACAAITQDKLGHSRAIYPLLEELPWPKAPTGLQGEIDR
                     ARRYSVSFLDEPLPSWSNVVAALALVGPAVNVVLEAVENSRYEALARRAHRILDEEKL
                     TSAYAEGLVRQISYEERGRRLFQERVDDLLPEMLCWFGPNGEPGIEALGAEGLTSMSN
                     EEMRQRYLDRIVPLLQGCGIEVPVERDESEGRWEYGELPWNQWNQLQRRLERS"
     gene            complement(995..1201)
                     /locus_tag="VFJ72_06770"
     CDS             complement(995..1201)
                     /locus_tag="VFJ72_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479202.1"
                     /translation="MEKYMVFARTEYDEPLEHRGDIEASDAEEASKLALERYGKDWLE
                     MSLVPEGQIYWAEREEATEVEVEA"
     gene            complement(1211..1408)
                     /locus_tag="VFJ72_06775"
     CDS             complement(1211..1408)
                     /locus_tag="VFJ72_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479203.1"
                     /translation="MRPVDNYEVFTARVGEEHPLRHNGNVRAAEPVDAAVYAHLMYDE
                     WRWREMFVVPSKAIVRVIRPE"
     gene            complement(1408..1731)
                     /locus_tag="VFJ72_06780"
     CDS             complement(1408..1731)
                     /locus_tag="VFJ72_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7479204.1"
                     /translation="MREERVSVTAEEARDALREVLDPEYPISLVDLGLIRGIEVNGNR
                     VDIKLTYTCMGCPAMDMIQEDVEERLLQMPGVEEVEIEVVWPTWSRKDITPLGRKQLK
                     QVGVV"
     gene            complement(1734..2687)
                     /locus_tag="VFJ72_06785"
     CDS             complement(1734..2687)
                     /locus_tag="VFJ72_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HET7479205.1"
                     /translation="MSTTAVTEEILLDRIKSGRLIEDSAQATERYIEGLKRTLIVSAD
                     TELISAPAYMRATRDFSTLPSVNNYITVMGIVQDELGHAHIAYRMLRDLGVDTEELVY
                     ERKPKQFKYPYAFDVPLESFTELVVANGFYDRAGFVLLSDIHRNCSYGPWKRALVKVD
                     REENFHLRHGEKWMRVFSKDPEKKEELQRAVDWMFVLTLEWFGLPDGKKRHNEQIEYG
                     YKGSTNDQLRQTWMSHTVPLCESLGLDAPAHYDEEQRKYVIDCPFPAQFDAEEKRWMF
                     EDGEISWDDVLKRWKDRGPNNEEYVEHLQRGYKELYGNGAV"
     gene            complement(2749..3630)
                     /locus_tag="VFJ72_06790"
     CDS             complement(2749..3630)
                     /locus_tag="VFJ72_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7479206.1"
                     /translation="MLDGIPLIDAHVHAARLPTLKPEWRDWARNFGKGVPHDELYDEE
                     GTILPARFDEYMADEGVDIAILFCEYSPKSTGIQPVEDLIPILEHNPERFRLMANLNP
                     HLHYPLVGDLKRQISLGAVGLKLHPVHGSFAPNDRMLYPAYAFCEAQDFPVVFHCGTS
                     VFPGATNRYADPALIEDVARDFPGLSVVLAHGGRGWWYDAAAFITLMRPNVYIEVSGL
                     PPKKLPDYYRNYDFERLARKMIFGTDWPGVPGPKVNAQAVANLGLDRETLEGILHKNV
                     RKVYKLEREQQKETRTA"
     gene            complement(3661..>4707)
                     /locus_tag="VFJ72_06795"
     CDS             complement(3661..>4707)
                     /locus_tag="VFJ72_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET7479207.1"
                     /translation="GKPKGAVHLHHDIIYTCETYARHVLEVSEDDMCFSASKLFHAYG
                     LGNGISFPYWAGASTVLFPGRPLPDAILGTIQRYRPTLFFSVPTLYNAILNHQGAEDY
                     DLSSIRLCASAAEALPAGIWRRWKEMFGSVILDGIGSTEMLHIFVSNAPDALKPGSSG
                     RPVPGYEAKILDEHGYPVEPGEAGYLSVKGDSAAAYYWRNHEKTKKTMQGEWLFAGDW
                     YRVDEDGFYWYEGRSDDMIKVSGLWVSPVEVESTLGDHPAVIEAAAVGVPVDGLTRVR
                     AHIVLRDGYEPSDELVTELQDWCKERLKRYQYPHLVDFVDELPKTVTGKIQRFKLREP
                     ETGVQMPQQEDELV"
BASE COUNT          757 a         1607 c         1411 g          932 t
ORIGIN      
        1 tagtcttcag gcccgcgtac tccatcatcg tttgatgact tcgaacgggc tgtgacactc
       61 ccggcagatg tacgagtaga ccatcaggtg cggcccgtac tcggagacca tctcgacgtc
      121 gccggagtcg caccacgggc aggccagaag ccgccgctcc tcttcctcgc cgacggggcc
      181 cgctacgatc tttctaagcg tctctggagt tggttccact ggttccacgg cagctctccg
      241 tactcccatc taccctcgct ctcatcgcgt tcgaccggga cctctatgcc gcatccctgg
      301 agcagcggga cgatccggtc caggtacctc tggcgcatct cctcgttgct catgctcgta
      361 agtccctccg ccccgagcgc ctctataccc ggctctccgt tgggaccgaa ccagcagagc
      421 atctcaggga gtaggtcgtc cacgcgctcc tgaaagagcc tgcggccgcg ctcctcgtag
      481 gagatctggc gcacgagccc ttccgcgtac gccgacgtga gcttctcttc gtccaggatg
      541 cggtgcgccc tgcgagctag tgcctcgtac ctggagttct ccacggcctc cagcacgacg
      601 ttcaccgccg gtccgacgag cgcgagcgcc gcgacgacgt tcgaccagct cggcagaggc
      661 tcgtcgagga agctgacgga gtaacgccgc gcccggtcga tctcaccctg gagccccgtg
      721 ggcgctttcg gccacggcaa ctcttcgagc agcgggtaga tcgcgcgaga gtgccccagc
      781 ttgtcctgcg ttatggcggc gcaggcggcg gttgactcca gcagcggacc ggcgtcggcc
      841 cactcggaga cgcgccgtcc caggaaatac tcgttgtctc ctataaccgc gatcagattg
      901 accagcgcgg cgagcgcgtg gtcgtcgtcc accaggaact cgctccccgc gccgcgcgac
      961 ggcgccactt cttcggtgac ttcgggacgc tcgctcacgc ttccacctcc acttccgtcg
     1021 cctcctcgcg ctcggcccag tagatctggc cctccggcac gagcgacatc tccaaccagt
     1081 ctttcccata acgctccagc gcgagcttcg acgcttcctc cgcgtccgag gcttcgatgt
     1141 cgccccggtg ctccaggggc tcgtcgtact cggtgcgcgc gaagaccatg tacttctcca
     1201 tcgctcctcc ctactcgggc cggatgaccc ggacgatagc cttgctcggc accacgaaca
     1261 tctcccgcca gcgccactcg tcatacatca ggtgcgcgta gacggcggcg tctaccggct
     1321 cggcggcccg gacgttgccg ttgtgcctga gcgggtgctc ttcgccgacg cgggccgtga
     1381 agacctcgta gttatccacc ggacgcatca gacgaccccc acctgcttga gttgcttcct
     1441 gcccagcggg gtgatgtcct tcctcgacca cgtgggccag acgacctcta tctctacctc
     1501 ctcgacgccg ggcatctgga ggagccgctc ctcgacgtcc tcctggatca tgtccatcgc
     1561 cggacaaccc atgcaggtgt acgtcagctt gatgtccacc ctgttcccgt tcacctcgat
     1621 gccccggatg aggccgaggt ccacgaggct gatcgggtac tccgggtcta ggacttcccg
     1681 aagtgcgtct cgcgcctctt ccgccgttac gctaactctc tcctcccgca tcgctacacc
     1741 gccccattgc cgtagagttc cttgtagccg cgctggaggt gctcgacgta ctcttcgttg
     1801 tttgggccgc ggtccttcca gcgcttgagc acgtcgtccc aggagatctc accgtcctcg
     1861 aacatccacc gcttctcctc cgcgtcgaac tgggccggga acgggcagtc tatgacgtac
     1921 ttccgctgct cctcgtcgta gtgagccggc gcgtcgagcc cgagggactc gcacagcggg
     1981 accgtgtggg acatccaggt ctgccttagc tggtcgttgg tcgagccctt gtaaccgtac
     2041 tcgatctgct cgttgtgccg cttcttgccg tccggcaacc cgaaccactc cagcgtcaag
     2101 acgaacatcc agtcaaccgc ccgctgaagc tcctctttct tctccgggtc cttcgagaac
     2161 acccgcatcc acttctcccc gtgccgcagg tggaagttct cctcccggtc aaccttcacg
     2221 agcgcccgct tccacggccc atacgagcag ttgcggtgaa tgtcgctcaa caacacgaac
     2281 ccggccctgt cgtagaagcc gttcgccacg accagctccg tgaagctctc cagcggaacg
     2341 tcgaaggcgt atggatactt gaactgcttc ggcttccgct cgtagacgag ctcctccgtg
     2401 tccaccccga ggtcgcggag catccggtag gcgatgtgcg cgtgcccgag ctcgtcctgc
     2461 acgatcccca tgaccgtgat gtagttgttc acgctcggca gcgtcgagaa gtccctcgtc
     2521 gcgcgcatgt aggccggagc gcttatcagc tccgtgtcgg cggagacgat gagagtccgc
     2581 ttcaaaccct cgatgtaccg ctccgtcgcc tgagcagagt cctcgatcaa cctcccgctc
     2641 ttgatcctgt cgagaagtat ctcctcggtt acagccgtcg tgctcacgcg tcctctcttt
     2701 cctggctctc ctgatgccat aacaatataa ctgaccggac agtcaaaatc aagccgttct
     2761 cgtctctttc tgctgttctc tctccagctt gtagaccttt cggacgttct tgtgcaggat
     2821 gccttccagg gtctcgcggt cgagaccgag gttggcgacg gcctgggcgt ttactttagg
     2881 tccggggacg ccgggccagt cggtgccgaa gatcatcttc cgggccagcc gctcgaagtc
     2941 gtagttcctg tagtagtcgg gcagcttctt cggcgggagg ccggagacct cgatgtagac
     3001 gttggggcgc atcagcgtga tgaaggccgc ggcgtcgtac caccagccgc gtccgccgtg
     3061 ggcgaggacg acggagagac cggggaaatc gcgggcgacg tcttctatca gggcggggtc
     3121 ggcgtatcgg ttcgttgcgc cggggaagac gctcgtgccg caatggaaca ccaccggaaa
     3181 gtcctgtgcc tcgcagaagg cgtatgccgg gtagagcatc cggtcgttgg gggcgaagga
     3241 gccgtggacg gggtggagct tcaggccgac ggcgcccaga gagatctggc gcttcagatc
     3301 cccgacgagc gggtagtgca ggtgcgggtt aaggttcgcc atcagccgga agcgctcggg
     3361 gttgtgctcc aggatcggga tcaggtcctc gacgggctgt atgccggtgg acttcgggct
     3421 gtactcgcag aacagaatgg caatgtccac gccctcgtca gccatgtact cgtcgaagcg
     3481 agcggggagg atcgtcccct cttcgtcgta aagctcgtcg tgcggcacgc ccttaccgaa
     3541 gtttcgtgcc caatcgcgcc actcaggctt gagcgtcggc aggcgggccg cgtgaacgtg
     3601 ggcgtctatg agcgggatgc cgtccagcaa aagtccgtcc tccttcccag gtgtcggtgg
     3661 ctagaccagc tcgtcctcct gctggggcat ctgtaccccg gtctccggct cgcggagctt
     3721 gaagcgttgg atcttgcccg tcaccgtctt cggaagctcg tccacgaaat cgaccaggtg
     3781 cgggtactgg taacgcttca accgttcctt gcaccagtcc tgaagctccg taaccagctc
     3841 atcggaaggc tcgtagccgt cacgcagcac gatatgcgcc cgcacccgcg tcaggccgtc
     3901 caccggcacg ccgacagcgg cggcctctat gacggcagga tggtctccga gagtgctctc
     3961 gacctcgacc ggcgagaccc agaggccgct caccttgatc atgtcgtccg agcggccctc
     4021 gtaccagtag aaaccgtcct cgtccacccg gtaccagtct ccggcgaaca accactcgcc
     4081 ctgcatcgtc ttcttcgtct tctcgtggtt gcgccagtag tacgccgccg ccgaatcgcc
     4141 cttgaccgag aggtatcccg cctcgccggg ctccaccggg tagccgtgct cgtccaggat
     4201 cttcgcctcg tagccgggca cgggtctgcc gctggaaccc ggcttcagcg catccggcgc
     4261 gttggagaca aagatgtgca gcatctccgt gcttccgatg ccgtccagga tcaccgagcc
     4321 gaacatctcc ttccagcggc gccagatgcc cgccggcagc gcctccgccg ccgaggcgca
     4381 gaggcggatt gacgagaggt cgtagtcctc cgcgccctgg tggttgagga tcgcgttgta
     4441 gagcgtcggc acggagaaga agagggtcgg cctgtaccgt tgaatggtcc ccagtatggc
     4501 gtcaggcagc ggcctgccgg ggaacaggac cgtcgaggct ccggcccagt aagggaacga
     4561 gatgccgttc ccgagcccgt aggcgtggaa gagcttcgag gccgagaagc acatgtcgtc
     4621 ctcggagacc tccagcacgt gccgggcgta ggtctcgcag gtgtagatga tgtcgtggtg
     4681 caggtggacc gcgcctttcg gcttgcc
//
LOCUS       DASSMB010000106         7500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_528399, whole genome shotgun sequence.
ACCESSION   DASSMB010000106 DASSMB010000000
VERSION     DASSMB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528399"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..1417)
                     /locus_tag="VFJ72_06800"
     CDS             complement(233..1417)
                     /locus_tag="VFJ72_06800"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7479208.1"
                     /translation="MREPLTFIRAFSLAATTALALLSGAVGACGPMNDAPQGDGQSTR
                     TITTSAANEPDVGSETTREKSPSNRIEVEVLAKDLDVPWGVAFLPDGDALVTERDSGR
                     LLRVSGDGKVEQVQRIPEDGSGEGGLLGLALSPDYEKDGLIYVYYSTGEDNRVGRFQL
                     GEQPEPILTGIPVNTYHDGGGIAFGPDGMLYVTTGDAGVPGNAQDRDSLSGKILRLTP
                     DGTVPDDNPFPDSPTYSYGHRNVQGLAWDESGQLYVSEFGQDRFDEVNKIEAGGNYGW
                     PEVEGAGGEPRFIDPIQTFATDEASPSGATILKDSAIPQWDGDFFMAALRGARLWRLE
                     LGPDGDVTGTEALLTGEYGRLRNAVQAPDGSLWIFTNNRDGRGTPTEGDDRILRLAPA
                     RG"
     gene            1580..2893
                     /locus_tag="VFJ72_06805"
     CDS             1580..2893
                     /locus_tag="VFJ72_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479209.1"
                     /translation="MLGAVFLVGYAWHTFAPGSLGTALFLGAGAVLFAMSVPWSSGFH
                     RGFALAAFAALGVVILMGRFHLSEFVEGMPAYFGIVAVLLVLSAAGYPIRAARYEVQI
                     RALMGALTRRGVGVKATSGALGHVLGAVLDVGSFVLIDVISGRAAPRERIDSLKWAAR
                     AFSFAPLWTNLNLLTATTITVTGVPYPGLLIVTLPFVFLGLAVTLLAAQREKGEVEDI
                     PDTPLDRGAAAVLLYPVLLVAAVAVVSLLFPRLTLTATIAITVAAVVLLIALIATAMT
                     RRASPITRLARETGRSLTVSYSEFALFGSAGILVLSLEALGALTPLGDLLSGLPIPLV
                     APALAVIMAVGFVCGIHVIPMVLLLNTAFPLDSGPAPALWAIAILLGSQAALLMTPFS
                     SSVTMLSRLSGLHPLVAGPKKNWKFGLVIALGSAVYLVLLTTLLL"
     gene            3085..3837
                     /locus_tag="VFJ72_06810"
     CDS             3085..3837
                     /locus_tag="VFJ72_06810"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin ligase family protein"
                     /protein_id="HET7479210.1"
                     /translation="MSTFLILLLFAVIFLAAGGALLYFRRSTQQKSGLMSQTETSSAA
                     DIGGLAPGTMVEVKGTLRCEEPLTSEIAGETCAYYTATVTREYLEEDHDDNDSSSDRR
                     SEVLSQIERFAPFTVEDASGAVPIDAEEAEVDARQVVERFERDTDSGFSLGGIPIRFG
                     DSERTIGYRYTESILPVNVPVYALGVVREDGKIGAPPSGDKEKRFIISYRSEEALGES
                     LGKTTFWLGVGAIGAFVLGIVLLVIGLFVLIM"
     gene            complement(3866..4870)
                     /locus_tag="VFJ72_06815"
     CDS             complement(3866..4870)
                     /locus_tag="VFJ72_06815"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7479211.1"
                     /translation="MTVAPSQLGTQPVGEVAKVAEGVYQLKVPVPIPLVFVSAYLIEG
                     DDGWTILDTGFDYPEGRAAWEAGAKSVGCDLREDVSRILVSHFHPDHLGAARWLQELS
                     GAPVYMLEDEIQHAHDIWGGDDGNFDLLIEHLTRHGMPRPLAEEAAAGTRANLKLPEK
                     MKPLTADEEIPLGGSAARVVHAPGHADHQYMLHDEARGILFAADHVLLKITPNIGRWP
                     DVMPHPLARYIESLDGVRGMDAGLVLPGHGPVFHDLDGRIDELLLHHEERLEIMRREI
                     LDSPKTSFEVSGRVFRDDLTLYEHCFALAETLAHLDHLVLEGRAERSENGVVTYRAAE
                     "
     gene            complement(4867..5751)
                     /locus_tag="VFJ72_06820"
     CDS             complement(4867..5751)
                     /locus_tag="VFJ72_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7479212.1"
                     /translation="MSGSPWNHQYLEANGIRLHYVRHGEGHPLVLLHGWPEFWYVWRK
                     NIPVLAEHFDVVAPDLRGFGDSDKPGLPDPPKKLLDDLVEDLRALADALGFERFGVVS
                     HDVGAYVSQGFARKYPERLSGLFFFNCPYPGIGDRWAAPGSINEIWYQTFNQQPWAAS
                     LVGENRKTCETYFRHFLDHWAHEPGLFDEDLDIWVDNFMKPGNLQGGFDWYISTNESR
                     LNLIRSGPPDLPKIPTPARVRWGEADSILKVEWTDRLGDYFTDLDFAPAPDAGHFVHY
                     ERPDFANREISDFFGRLA"
     gene            complement(5791..6969)
                     /locus_tag="VFJ72_06825"
     CDS             complement(5791..6969)
                     /locus_tag="VFJ72_06825"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET7479213.1"
                     /translation="MREAVIVSGARTAVGRAPRGTLRAMHPVDMTAAAIREAVNRAEG
                     LDPTDVEDVIIGCAMPEGTQGNNMARLASLRAGMSETVPAQTVNRFCSSGLQTIANAA
                     ERIMTGQAQTILAGGCEHMSTTIEMPNFSPAPSLVETNPAVYMGMGLTGEQVAKEFKV
                     SREDQDEHALRSHDRARKAVSSGLFDEEIVPLDVEYDYVDDNGEVQHFQTTFRRDEGP
                     RDTSLEQLAKLPTVFQRGGTVTAGNSSQRSDGAAAVVVMDREEAERRGLQPLARFVGF
                     AVGGVRPEVMGVGPMVAIPKALKIAGLTIDDIDLVEFNEAFAAQFLAVVREVGLDIEK
                     TNVNGGAIALGHPMGATGTKLTVQLIHEMKRRESKYGMVTMCIGGGMGAAGIFENLQN
                     "
     gene            complement(6988..>7500)
                     /locus_tag="VFJ72_06830"
     CDS             complement(6988..>7500)
                     /locus_tag="VFJ72_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HET7479214.1"
                     /translation="RRLVSRALRVTPNIPPLPLVQKAFEQIAMAKVSASALEAREMGY
                     LEEDDRVVMNPDHLLSAAKREVHDLADGYAPPEHGKNVYAVGKTTRAALEVGVRTLQW
                     GRYASEYDGVIAGHLARVLTGGDLGQPQWVSEEYVLNLEKEAFLKLLENEKTHERIGG
                     LLKTGKPVRN"
BASE COUNT         1176 a         2521 c         2376 g         1427 t
ORIGIN      
        1 tccaccggga cggcgcgact ccgccttgcg acagacgcgc cgtcccgcaa gagtcctcga
       61 atatcgggga gtcacgcgga tagtgaaata tacgaccccg gactcccaac acgcccattc
      121 gacgccttcc gccctttgtt ccgtcgctct tctcacgtag ggctcgaccg gacaccggga
      181 tgctccagct atgtccgcca agagccggaa gctctacacg aaccgcagca ttctagccgc
      241 gtgcgggagc gagccgcagg atacggtcgt caccctcggt gggcgtgccg cgtccgtcgc
      301 ggttgttggt aaagatccac agagaaccgt cgggggcctg aacggcgttc ctgagccgcc
      361 cgtattcgcc tgtcagcagg gcttccgtgc cggtcacgtc gccgtccggg cccagctcca
      421 ggcgccagag ccgcgctccc cggagggccg ccatgaagaa gtcgccgtcc cactgtggga
      481 tggcgctgtc cttcagaatt gtggctccgc tcggggaggc ttcgtcagtg gcgaaggtct
      541 ggatcgggtc tatgaacctc ggctctccac ccgcgccctc gacctcgggc cagccgtagt
      601 taccgccggc ctctatcttg ttgacctcgt cgaagcgatc ctggccaaac tcacttacgt
      661 acaattgtcc gctttcgtcc catgcgagac cctgcacgtt gcggtgtccg taggagtacg
      721 tggggctgtc cgggaagggg ttgtcgtccg ggacggtgcc gtcgggggtg aggcgcagga
      781 tctttccgga gagcgagtcg cggtcctggg cattcccggg cactccggcg tcgccggtcg
      841 tgacgtagag catcccgtcc ggcccgaatg cgatgccgcc tccgtcgtga taggtgttca
      901 ccgggatgcc ggtcagtatg ggctcgggct gctcgccgag ttggaagcgt cctacgcggt
      961 tgtcctcgcc ggtggagtag tagacgtaaa ttagcccgtc cttctcgtaa tccggggaga
     1021 gcgccagccc cagcagtcca ccctctccgc taccgtcctc cggtatccgc tgcacctgct
     1081 cgaccttgcc gtccccgctc acgcgcagta gtcggccgga gtcccgctcc gtgaccagcg
     1141 cgtcgccatc cggaagaaac gctactcccc acggtacatc caggtccttg gccagcacct
     1201 cgacctcgat gcggttggaa ggactcttct cccgcgtggt ctcagagccg acatctggtt
     1261 cgttcgcagc ggaggtggtt atggttcggg tactctgtcc gtcaccttgc ggcgcgtcgt
     1321 tcatcgggcc gcaagcgccg acagcgcctg agaggagcgc cagcgcggtg gttgctgcga
     1381 gcgaaaaagc tcggataaaa gtcaacggtt cccgcattcg gctccttctg gggcgtacgc
     1441 cgctgattat attgcccgca acctgtaacc tgatacccgc aacgatggat ctaccagagg
     1501 acggcccgaa gtcagcgaga atccttattc atcgtcccgg cgggagaatc tgaagttacg
     1561 ccttgacagg ggacgcccgg tgttgggcgc ggtcttcctg gtcgggtatg cgtggcatac
     1621 gttcgcgccg gggtctctcg ggacggcgct gttcctggga gcgggggccg tgctgttcgc
     1681 catgtccgtg ccgtggtcga gcggtttcca tcggggcttc gcgctggccg cgttcgctgc
     1741 tctgggagtc gtgatcctga tgggacggtt tcatctttcg gagttcgtcg agggtatgcc
     1801 ggcttacttc gggatcgtgg cggtgctgct ggtactgagc gcggcgggct atcccatccg
     1861 ggcggcgcgc tacgaggtcc agatacgggc cctcatgggc gccctgactc gccgcggcgt
     1921 tggcgtgaag gcgacgtccg gagccctggg acacgtgctg ggagcggtgc tcgacgtcgg
     1981 gtcgttcgtc ctgatagacg tgatctccgg ccgcgccgcg cccagagagc ggatcgactc
     2041 cctcaagtgg gccgcgcgcg cgttctcgtt cgccccgctc tggacgaacc tcaacctcct
     2101 caccgccacc accataacag tcaccggcgt cccctatccc ggcctgctga ttgtgacgct
     2161 gcccttcgtc tttctcggcc tcgccgtcac actcctggcc gcccagcgcg agaagggtga
     2221 ggtggaagac atcccggaca cgccgctcga ccggggcgcg gccgcagtgc tcctctatcc
     2281 ggtgctgctc gtcgcggcgg tggccgtggt gagcctgcta ttccccagac tcacgctgac
     2341 tgccaccatc gccataacgg tcgcggccgt cgtgctgctc atagcgctca ttgccaccgc
     2401 catgacgcgc cgggcttccc ccatcacccg gctggcgcgg gagacgggaa ggtccctcac
     2461 cgtctcgtac tcggagttcg cgttgttcgg ctccgctggg atactggtgc tctccctcga
     2521 agctctcggc gcgctcacgc cgctcggaga cctgctttcg ggcctgccca taccgctcgt
     2581 ggctccggcc ttagcggtca tcatggcggt cgggttcgtc tgcggcatcc acgtcatccc
     2641 gatggtactg ctcctgaaca ccgcattccc cctcgatagc ggaccggccc ccgcgctatg
     2701 ggccatcgcg atcctgctcg gttcccaggc cgccctcttg atgacgccct tctcaagctc
     2761 cgtcaccatg ctctcccggc tctcggggct gcacccactg gtggcgggtc ccaaaaagaa
     2821 ctggaagttc ggtctcgtca tcgccctggg ctcggctgtc tatctcgtcc tgttaacaac
     2881 cctgcttctg tagaatgtcc gcctgtgccc tcgcggtgct ccacccggcg gttcagaccg
     2941 gagagatcgc aggtcaagag ccggagccta tgtgaagcct tcgacccacc gagaatagtc
     3001 catttgcgtg atgcagatag cgctgcgaat gaggtacagt cggaaaacga acttgtatcc
     3061 cgcagataga aagggacttc caagatgtcg actttcctga tcctgttgct gttcgcggtc
     3121 atcttcctgg cggctggcgg cgcgctgctc tacttccgga gaagcacgca gcagaagtcc
     3181 ggccttatga gccagaccga gacctccagc gccgctgata taggcggact ggctccaggg
     3241 acgatggtcg aggtcaaggg gacactgcgc tgcgaggaac cgctcaccag cgagatagcc
     3301 ggcgagacgt gcgcctacta cacggccacg gtgacgcgcg agtacctgga agaagaccat
     3361 gacgacaatg actctagctc ggaccggcgc tcggaggtcc tctcgcagat cgagcgcttc
     3421 gctccgttca cggtcgagga cgcttcgggc gcggttccca tagacgccga ggaagccgag
     3481 gtggacgcga gacaggtggt ggaacgcttc gagcgggaca cggactccgg gttctcactc
     3541 ggcggaatcc cgatacgctt cggagacagc gagcgcacta tcggctaccg ctacacggag
     3601 agcatcctgc cggtaaacgt tcccgtctac gcgctgggcg tggtgcggga ggacggcaag
     3661 atcggggctc cgccatccgg agacaaggag aagcgcttta ttatcagcta ccgctcggag
     3721 gaagctctcg gcgagagcct cggcaagacg acgttctggc tcggagtcgg cgccataggc
     3781 gcgttcgtgc tgggcatcgt cctgctcgtg atcggcctct tcgtactgat tatgtaaggg
     3841 cgctaccgcc gatcggagcg aacggctact ctgcggcccg gtaggtgacc accccgttct
     3901 ccgaacgctc cgcccgtccc tccaggacta gatggtccag gtgcgccagg gtctcggcga
     3961 gcgcgaaaca gtgctcgtaa agggtaaggt cgtcccggaa gactctgccg gagacctcga
     4021 aggaggtctt ggggctatcc aggatctcgc ggcgcatgat ctcaagccgc tcctcatggt
     4081 ggaggagcag ctcgtctatc ctgccgtcga ggtcgtggaa gacgggaccg tgtccgggca
     4141 ggacgaggcc cgcgtccata ccgcgcactc cgtcgaggga ctcgatgtag cgggcgagcg
     4201 ggtggggcat gacgtccggc cacctcccga tgttcggcgt gatcttgagc agcacgtggt
     4261 ccgcggcaaa caggataccc cgcgcctcat cgtggagcat gtactgatga tccgcgtgtc
     4321 cgggagcgtg cacgacccgc gccgcgctcc cacccagcgg gatctcctca tccgctgtca
     4381 acggcttcat cttctccggg agcttcaggt tcgcccgcgt cccggcggcc gcttcctcgg
     4441 cgagcggacg tggcatcccg tgccgcgtca ggtgctcgat gagcaaatcg aagttgccgt
     4501 cgtctccgcc ccagatgtcg tgggcgtgtt ggatctcgtc ttccagcatg tacaccgggg
     4561 cgccggagag ctcctgcaac caccgcgccg ccccgaggtg gtccggatgg aagtggctca
     4621 ccaggatgcg gctcacgtcc tcgcgcaggt cgcacccgac gctcttcgct cccgcttccc
     4681 aggccgccct tccctccggg tagtcgaagc cggtatccag tatggtccag ccgtcatcgc
     4741 cctcgatcag gtacgccgag acgaacacca gcgggatcgg aaccggaacc ttgagctggt
     4801 agacaccttc cgcgaccttc gcgacctctc ccaccggctg cgtcccgagc tgagacggcg
     4861 cgacggtcac gcaagcctcc cgaagaaatc ggagatctca cggttggcga agtccggccg
     4921 ctcgtagtgg acgaagtgcc cggcatccgg cgccggggcg aagtccaggt ccgtgaagta
     4981 gtctccgagc cggtcggtcc actccacctt gagtatgctg tctgcctcgc cccaccgcac
     5041 gcgagcgggc gtcggtatct tcggcaggtc ggggggaccg ctgcggatga ggttcagccg
     5101 ggactcgttg gtgctgatgt accagtcgaa cccgccctgg aggttaccgg gtttcataaa
     5161 gttgtccacc cagatgtcga ggtcctcgtc gaagaggccg ggctcgtgcg cccagtggtc
     5221 gaggaagtgg cggaagtacg tttcgcaggt cttccggttc tcgcccacca gggaggcggc
     5281 ccacggctgc tggttgaagg tctggtacca gatctcgttt atgctccccg gcgccgccca
     5341 gcgatccccg atccccggat agggacagtt gaagaagaac agccccgaca ggcgctccgg
     5401 gtacttccgg gcgaaaccct gagacacata ggcgcccacg tcatggctca cgaccccgaa
     5461 cctctcgaag cccagagcgt ccgccagggc gcgcagatcc tctacgagat cgtccagcag
     5521 cttcttcggc gggtcgggca gacccggctt gtccgagtcg ccgaaacccc ggaggtccgg
     5581 cgcaaccaca tcaaaatgtt cggcgaggac cgggatattc ttccgccata cgtaccagaa
     5641 ctcgggccat ccgtgcaaca gcaccagtgg atgcccttcg ccgtgacgga cgtagtgaag
     5701 gcggatgccg ttcgcttcga gatactggtg gttccacggg gatccgctca ccgcaactcc
     5761 ctcatgctga cacgccaacc ggctaccaag ctaattctgc aggttctcga agataccggc
     5821 cgcgcccatg ccgccgccga tgcacatcgt gaccatcccg tacttcgact cgcggcgctt
     5881 catctcgtgg atgagctgga ccgtgagctt ggtgccggtc gcgcccatcg ggtggccgag
     5941 cgcgatagcg ccgccgttca cgttggtctt ctcgatgtcc agcccgacct cgcgcaccac
     6001 ggccaggaac tgcgccgcga acgcctcgtt gaactcgacg aggtctatgt cgtcgatagt
     6061 gagaccggcg atcttgagcg ccttcgggat ggcgaccatc ggcccgacgc ccatgacctc
     6121 cggccgcacc ccgccgaccg cgaacccgac gaagcgcgcc agcggctgga gcccgcgccg
     6181 ctcggcctcc tcccggtcca tgaccacgac cgccgccgcg ccgtcgctgc gctgggagga
     6241 gttcccggcc gtcaccgtac cgccccgttg aaagacggtc gggagcttgg cgagttgctc
     6301 cagagaggtg tcgcgtggcc cctcgtcgcg cctgaaggtc gtctggaagt gctggacctc
     6361 gccgttatcg tcaacgtagt cgtactccac gtcgagcggc actatctcct cgtcgaagag
     6421 cccgctgctc actgctttcc gggcgcggtc gtggctgcgg agggcgtgtt cgtcctggtc
     6481 ctcgcgcgat accttgaact ctttcgcgac ctgctcgccg gtcaggccca tccccatgta
     6541 gacggcgggg ttggtctcga cgagcgaggg ggccggggag aagttcggca tctcgatggt
     6601 cgtggacatg tgctcgcagc cgccagccag gatcgtctgg gcctggcccg tcatgatcct
     6661 ctccgccgcg ttcgcgatgg tctgaagccc ggaggagcag aagcggttca cggtctgcgc
     6721 cggtacggtc tcgctcatcc ccgccctgag cgaggcgagc cgggccatgt tgttgccctg
     6781 cgtcccctcc ggcatcgcgc agcctatgat cacatcctcc acgtccgtgg gatccagccc
     6841 ctccgcccga ttgacggcct cccggatcgc ggcggcggtc atatcaaccg ggtgcatggc
     6901 ccgcagcgta cctctcggcg cccgcccgac ggcggtacgc gccccggaga ctattacggc
     6961 ttctctcata tctttacctc tctcgctcta gttccgtacc ggcttgccgg tcttgagcag
     7021 accgccgatt cgctcgtggg tcttctcgtt ctccaacagc ttgaggaacg cttctttttc
     7081 aaggttcagg acgtactcct cgctcaccca ctggggctgg ccgaggtcgc cgccggtgag
     7141 gacccgcgcg aggtgtccgg cgatgacgcc gtcgtactcg ctggcgtacc ggccccactg
     7201 caacgtcctt acccctactt ccagggctgc gcgggtcgtt ttcccgacgg catagacgtt
     7261 cttgccgtgt tcgggcgggg cgtagccgtc ggcgagatcg tgaacctcgc gcttcgcggc
     7321 agagaggagg tgatccgggt tcatcacgac gcggtcgtcc tcttccaggt agcccatctc
     7381 ccgcgcttcg agggcgctgg ccgagacctt cgccatggct atctgctcga atgccttctg
     7441 caccagcggc agcggcggga tgttcggggt cacgcgcagc gcgcgggaga cgagcctgcg
//
LOCUS       DASSMB010000107        10035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1289019, whole genome shotgun sequence.
ACCESSION   DASSMB010000107 DASSMB010000000
VERSION     DASSMB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289019"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(531..722)
                     /locus_tag="VFJ72_06835"
     CDS             complement(531..722)
                     /locus_tag="VFJ72_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479215.1"
                     /translation="MLITASIVGVLLLAELLLTLLGQGGVRTEFIEFAVALVMMLVIL
                     VGSYWVIVGGSGRGSGRDT"
     gene            complement(1026..1331)
                     /locus_tag="VFJ72_06840"
     CDS             complement(1026..1331)
                     /locus_tag="VFJ72_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479216.1"
                     /translation="MRSIRYGSGKVFSGRKKPLLVPDPGQKVELITEDGSTRTGFRAV
                     SGPLTSNTGEAVVRVATEEEYREAEIEGRRPVGLAWPAARLEIPVPRRWRRMHQLAR"
     gene            2112..3608
                     /locus_tag="VFJ72_06845"
     CDS             2112..3608
                     /locus_tag="VFJ72_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7479217.1"
                     /translation="MTTEARNTGSVESPHLRRELHLWEAVGISLALMAPSMAANINPQ
                     GTAGSVGRAVPLAFALATIGVLLIAWTFVRLTQRFNHAGSVYGFVGATLGARTGVISG
                     WALMGTYTFYGVVTSTAAGIFGADFLDALGIWKNQPGWAPFLVGAIALAGVWALAASN
                     IREGTRVLLVIEGTTVALILIVSVIILIRLATGTAPNGNTLDFSVFTVPGGTGLSAVF
                     LGVVFGFLSFAGFEAAATLGEEAQEPRRDIPRAILGVAIFGGLYFIFVTAIEVMGFGT
                     DAKGVKEFIASGSLLGDLGSRFVAGWVGELITIGAAVSAFGCALACVVGAARLLYALS
                     RDEVGPASLGAVSSRSGVPARSTAAVAIAAYVITALGWFVFGVAPFDLFVASGTIGTL
                     ILLLVYALATIGAAKLLFFSGERQVAAWEVIVPLLALLVIGYTLFRNVYPYPTEAAAW
                     YPIIAALWVIVGVLITFIRHAATQRAGERLMAEEGLTAGAGSRPEGYR"
     gene            3685..5025
                     /locus_tag="VFJ72_06850"
     CDS             3685..5025
                     /locus_tag="VFJ72_06850"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET7479218.1"
                     /translation="MQTEREAQENVARPLPEGTLWVRFVFVDHAGIPKCKAVHRDGFA
                     GRARAGVGLAKGVLALDPSGALHPESGLGPVGECRLVPDLSTLTALPFARGQAMVSCD
                     MTEPDASTPWEGCPRSALKRVLGRLSERGYRSLASYEAEFYVWNSDGPLDRTPYAGSF
                     ALTSAADFVAELAQTLEEMGIRPEQCHAEVGHGNLELSVGEDEALAAADKRVMVLEAI
                     RGVAHRMGLQITMAPKPYLDGAGNGHHLHVSLYEDDAPVLFDASGALSGPGSGFVAGI
                     LEHLPAVMAFTAPSPNSYQRLAPGMWSSAFGAYGLDNREAAVRLASPVAGRESATANV
                     ELKPVDVTSNPYLALAAVLAAGMDGIERRLDPGEPAMVDPATMGDEERASRGIRPLPA
                     SPDEALNALEEDEVLIEALGGPLVRTHVAVARAQAAIARELAPEEVAASAAALY"
     gene            5045..5866
                     /locus_tag="VFJ72_06855"
     CDS             5045..5866
                     /locus_tag="VFJ72_06855"
                     /inference="COORDINATES: protein motif:HMM:NF044879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479219.1"
                     /translation="MGPLAGAGGILSGPRDFALLHERAMPQKDQLCGAFWGALVLSAA
                     GHAGTQEQVALRAGTTLAEGDPESWLPPGAAPRTDYEAAIPFASDEASAGTSATGVAQ
                     SIEELSGGTLSVVPVAGPWSAETVVSLVDLVAATAPECVLISNLRTGHLWGSRPSARL
                     LLDYLLGRPVEAPPPDWDCGHFLTIAARIGGPGGALVVLRDTYPQLGRDGYHLQPAGA
                     VAAALERGDGHEGGVLCACETPAAGALAGRLGSEGFELRHWDNGSPDRGGETSAG"
     gene            6018..7196
                     /locus_tag="VFJ72_06860"
     CDS             6018..7196
                     /locus_tag="VFJ72_06860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7479220.1"
                     /translation="MSADLLRTLLGALEEELPRAVRLRERLHAIPEPSHEEHATARLV
                     AEALGTGVVKRVARTGLMAHAGPPGEAVAVRAELDALPIEEASGVPFAATNGFMHACG
                     HDVHMAALAALFWAAGRVGGSLPKPLAALYQPSEEAYPSGADLVVREGALARGTAAVV
                     AAHVHPEVPWGSVSVEAGPVNASSDNLRIIVEGAGGHGAYPHRARDPVLALSSIIVAL
                     QSLVSRRLDPAHAAVFSVGWLRAGSAENVIPNAAEARGTLRALDPGDRAPLREMAREI
                     AAHTARAHGCAASVEVTEGEPATVNDPALAEAARSLLPEAGFELAPAMRSCGSDDFGF
                     YGRVSPTLMMFVGLKSGPDTPDVPLHHPRFLPPDEAVPAVARAQAVAFAAAASLDPAP
                     "
     gene            7284..8036
                     /locus_tag="VFJ72_06865"
     CDS             7284..8036
                     /locus_tag="VFJ72_06865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET7479221.1"
                     /translation="MVGRLEGRAAVVTGGARGIGRGIVEAFAAEGADVAVADTLPERE
                     AAAVLAEVERAGRRGVYVHTDVAEEYQVRNMVDTVLGAFGRIDVLVNNAGVLSQVYVE
                     EMPVEEWDRVLRVNLRGTFLCCRYVLPHMLERGEGRILNIASQIGQSGAPGLAHYSAS
                     KGGVIAFTKALAREVAARGVLVNAIAPGPISTGIMPSQSEELEEARKAALPIRRFGTV
                     DEVAPTAVFLASGDSSYYVGQTLGPNGGDVMF"
     gene            8050..9189
                     /locus_tag="VFJ72_06870"
     CDS             8050..9189
                     /locus_tag="VFJ72_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7479222.1"
                     /translation="MEAVDLTQIPVVDNHCHGIRPDQTFECLASWRRAFTESTDPGMP
                     RDHVATTVFYRRLIQTLASFLGCEPDEEAVLAARNGIEGRKLTGDLLRAANVDTLLLD
                     TGFPPPEEVFPVPELGELGGCRAEPMLRLEVLMESLLSEHNSLAGTEEALAAALEDVR
                     GGGYVALKSIAAYRTGLDIREWTREEAEASFDEYRRAAGEGSARLVHKPLLDTLLHVA
                     FAEAARQEVPVQFHAGYGDADTDLLLGNPLYLRPVLQRPDYRGMPVVLLHECYPYTRQ
                     GGYLAAVYENVYLDLSYGIPLLGYSEMLSFTRAALGVAPASKLMYSSDGIGVPELHWM
                     SAADGRRVLGEALGELVANGELGLPEAEAAGEGVLRANAARLYRL"
     gene            9388..9789
                     /locus_tag="VFJ72_06875"
     CDS             9388..9789
                     /locus_tag="VFJ72_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479223.1"
                     /translation="MGLGAEPGVGGSECEAQDSMHFMGQGIAIPEMADSATGRSIVPV
                     RGAIGELVYTAIHRECTPLIRPRMNDIIEVVDDAPSRAAGRGSPLPHRCPGVNRSRSR
                     TVPSGREGVKYDLTSRSVGDLDLSEHDKRQG"
     gene            9808..>10035
                     /locus_tag="VFJ72_06880"
     CDS             9808..>10035
                     /locus_tag="VFJ72_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HET7479224.1"
                     /translation="MPRTGFRLELDGRSLKIEDVVAFARGRGDAECILSAESMEKIEA
                     SCALKQDLISRGIPIYGVTTGFGDSANRQISP"
BASE COUNT         1608 a         3279 c         3464 g         1684 t
ORIGIN      
        1 tcgccgcaat taccggtgga ttgttattag tggacataga ggtagagttc ccgctccgag
       61 aacacctgct caagcagcac tcttagctcc ttcggcgagg gtagatcgga gtgcgccctc
      121 cagtatcgtc aaagcatcac ctgcggctcc agccaccctc ttactgcttt caaggtctcc
      181 ggccaggata ccgaaatcct cctacttttg tctcctctac gagccggccg gtaggtcgtc
      241 ctctgttcgc tcgacggact tttccggcaa agccggcaag cgactgtaag aggctccgtc
      301 aatatgcgag tagcatatgt gtcaagaaac aatgagaagg ctgttgaatc ggtctgagag
      361 ctgccaagta cggaaagtgt ccagcctatt gacggaagct ctagagtcag tggttctcgt
      421 tcggctatct gctggcggta gccttgagcg tcctggtgat agtcctgctt gtattctggc
      481 tggtataggt tcgcagggtg atgctctcca ggccagagcc tttgcggata ctacgtgtct
      541 ctgcccgaac ctctgccgga cccgcccacg ataacccagt acgacccgac caggatgacc
      601 agcatcataa ccagggccac ggcgaactct ataaactccg tccgcacgcc cccctgtccc
      661 agcagcgtca ggagaagctc ggcaagcaac aagaccccga ctatagaggc ggttataagc
      721 actgtcctca acatattgaa acaattataa cagcagagcc tcttcaagtg cagcaaggtg
      781 tggaggttgg atccaacccg caatagttcg ctctctcgct tgtgagcgtc tgacctgctt
      841 cggaagcacc ggaggctggg cattgaaaaa gggccggggc tgtactcaca atcttaatgc
      901 gcaccgctgt cagttgactg ctcctgaacc cagtcgtaca gtccgtgagg gctctctgcg
      961 gggcgcggat ccggttcatg gtgccagcgc cgctgtggct acctgacatg gccggggcta
     1021 tcagcctatc tggcgagctg gtgcatccgg cgccaccgcc ggggcaccgg gatctccagc
     1081 cgcgctgcgg gccaggccag gcccacggga cggcgaccct cgatctcggc ctcccggtac
     1141 tcctcctcgg tggcaacccg gaccaccgcc tcacccgtat tcgaggtaag cggacccgag
     1201 acggccctga agccggtcct tgtagacccg tcctccgtga tcaactcgac cttctggccg
     1261 ggatccggca ccagaagtgg cttcttcctg ccggagaaca ccttgcccga accgtatcga
     1321 atgcttctca taggcccggt attttacaga tccacaaccg gttgcgtagg atctataaaa
     1381 tgatgactgt atccgggtga tgtccggccc accacatgac tcgcgacggg gccagacctg
     1441 tctcaagtag agggtggtag agcatcgggt ccaaggtgag gcttcgcggt cccgcagccc
     1501 tgcgcggccg gggagcccaa gattctcgaa ggttcgcgga gaacatcgct ttcatcgtcg
     1561 tggacggacg cgcggaacat gcttcgcggc tggatcccct ccgcgcaact gcctttcccg
     1621 atctcgcgcc cgtcacgcgg ggtggaagta ctttccaggt ctacacctgc cacgaggcag
     1681 cagagagaga tcacacccgc aagcctcaaa cggccttccc cggcttcgac gaccctcgcc
     1741 aggctttccg ccaggatcgc gacgcgctcg cactcctgcg ccaattgccg gcgggctgct
     1801 ggaccgccct caggaactta tcagagagtt gcgcgcgtga acctgacaat gcactatcca
     1861 ggaggctatg atacttgcgg gtagaaaggt cgtctggtct ggggaccggg ccggaaaggg
     1921 gaagacatgg tttgcagcgg ggacacccac tctcttctcg atgaagcctg tgtcgaggcc
     1981 ctttgcctct cgccccggga cgggcctgtc aaagggccgg atcatgcggg caccggcgcg
     2041 gatgaggtga gtatgggact ggcctgaccc cgaccgaccg caccccgcaa ccaccgagca
     2101 agggagtcac tatgaccaca gaagcgagga acaccggaag cgtagaaagt ccgcacctgc
     2161 ggcgcgagct gcacctgtgg gaggcggtcg gcatctcgct ggccctgatg gcgccgtcga
     2221 tggccgccaa catcaacccg caggggaccg ccggcagcgt cggacgcgcc gtgccgctcg
     2281 cgttcgcgct ggcaaccatc ggggtgttgc tcatagcctg gaccttcgtc cggctcaccc
     2341 agcgcttcaa ccacgctggc agcgtgtacg gtttcgtcgg ggcgacgctc ggggcgcgga
     2401 ccggtgtgat ctcgggttgg gcgctgatgg gcacgtacac tttctatggc gtcgtcacgt
     2461 ccaccgcggc cggcatcttc ggcgctgact tcctcgacgc cctcggcatc tggaagaatc
     2521 agccaggctg ggcgccgttc ctcgtcggcg ccatcgccct cgccggcgtg tgggcgcttg
     2581 cggcctcgaa catccgggag ggcacccggg tgctgctggt catcgagggg acgacggtcg
     2641 cgctcatcct catcgtcagc gtgatcatcc tgatccggct tgcgaccggc acggctccga
     2701 acggcaacac tctggacttc tcggtgttca cggtgccggg agggacgggc ctgtcggctg
     2761 tgtttctcgg ggtggtgttc ggattcctct ccttcgccgg gttcgaggcg gccgcgacgc
     2821 tcggtgagga ggcgcaggag ccccgtcgcg acataccgcg cgcgatcctg ggcgtcgcca
     2881 tcttcggcgg cctgtacttc atcttcgtta cggccatcga ggtgatgggc ttcgggactg
     2941 atgccaaggg agtcaaggag ttcatcgcct ctggctctct gctcggcgac ctcggaagcc
     3001 ggttcgtcgc cgggtgggtc ggcgagctta taaccatcgg cgccgctgtc agtgccttcg
     3061 ggtgcgcgct ggcttgcgtc gtgggggccg cccggctcct gtacgcgctc tcgcgcgacg
     3121 aggtcggccc ggcctcactc ggcgccgtct catcccggag cggcgtcccg gcccgctcca
     3181 cggccgccgt cgccatcgcc gcgtacgtca tcaccgccct cggctggttc gtgttcgggg
     3241 tggcaccctt tgacctgttc gtggcatcgg gcactatagg cactctgatc ctgcttctcg
     3301 tctacgcgct cgcgaccatc ggggccgcga agctgctgtt cttctctggc gagcggcagg
     3361 ttgctgcgtg ggaggttatc gtgcctctac tcgctctgct cgtcatcggc tacacgctct
     3421 tccgaaacgt gtacccttac ccgaccgagg cggcggcatg gtacccgatc atagccgcgc
     3481 tctgggtcat cgtgggggta ctcatcacgt tcatccggca cgccgcgacg cagcgggccg
     3541 gcgagaggct tatggccgag gaagggctaa ccgcaggagc cggctccagg ccggagggct
     3601 accggtaggc ggcccggcgc cgcaaaacgc gatggcgcat catcgcggga aggaaaggta
     3661 ttcagctaca tcataggggg tgcgatgcag accgagcgcg aggcgcagga gaacgtggcg
     3721 agacctctac ccgagggtac tttgtgggtc cggttcgtgt tcgtggacca cgcgggcata
     3781 cccaagtgta aggccgtcca ccgggatggc ttcgccggaa gggcgagggc cggggttggg
     3841 ctcgcgaagg gtgttctggc actcgacccg agcggggcgc tgcacccgga gtcggggcta
     3901 ggccccgttg gagagtgccg gcttgtcccg gacctctcga cgctcaccgc cctgcccttc
     3961 gcccgtgggc aggcgatggt ctcctgcgac atgacggagc ccgacgcgag cacgccctgg
     4021 gagggctgcc cgcgcagcgc cctcaagcgg gtgcttgggc ggctctcgga gagaggctac
     4081 aggtcgctcg catcctacga ggccgagttc tacgtgtgga actccgacgg ccccctggac
     4141 agaacacctt acgcggggag cttcgcgctg acgtcggcgg cggacttcgt cgcggagctg
     4201 gcccagacgc tggaggagat gggtatccgt ccggagcagt gccacgccga ggtcggacac
     4261 gggaacctgg agctctccgt gggggaggac gaagcgctgg ccgcggcgga caagcgcgtg
     4321 atggtgctgg aggcgatccg gggcgtggcc caccggatgg ggctgcagat cacgatggcc
     4381 ccgaaacctt acctggatgg ggccggcaac ggccaccatc tgcacgtgag cctctacgag
     4441 gacgacgctc cggtcctctt cgacgcctcc ggagccttga gcggaccggg ctcgggcttc
     4501 gtggccggga tactggagca tctgcccgcc gtcatggcgt tcaccgcccc gagccccaac
     4561 tcctatcagc ggctcgcccc aggcatgtgg tcctccgcct tcggcgctta cggcctggat
     4621 aacagggagg ccgccgttcg gctcgcctcg cccgtggccg gcagggagtc agcgacggcc
     4681 aacgttgagc tcaagccggt cgacgtcacg tcgaacccgt atctcgcgct ggcggcggtc
     4741 ctggccgccg ggatggacgg gatcgagcgc cgcctggacc ctggggagcc ggcgatggtg
     4801 gacccggcca cgatgggcga cgaggagcgt gcctcaagag gcatccggcc gctccctgcc
     4861 tccccggacg aggcactgaa cgcgctggag gaagacgagg tcctcataga ggcgctgggt
     4921 ggtccgctgg tgcgcaccca cgtggccgtg gcgcgggcgc aggccgcaat cgccagagag
     4981 cttgcgcccg aggaggtggc ggcctcggcc gcggctctct actgaaagga gttgtgtctt
     5041 gctgatgggt ccgctagccg gggcgggcgg tatcctctcc ggcccccgcg acttcgccct
     5101 actgcacgag agggcgatgc cgcagaaaga ccagctctgc ggcgcgttct ggggggcgct
     5161 ggtcctctcg gccgccggac atgcgggcac ccaggagcag gtcgccctca gggccggaac
     5221 gaccctggcc gaaggagacc ctgagagctg gctgccgccg ggcgccgcgc cccggacgga
     5281 ctacgaggcg gccatccctt tcgcttccga cgaggcttcc gcggggacgt cagcgacggg
     5341 ggtcgcgcag agcatcgagg agctctccgg gggcactttg tctgtcgttc ccgtggcggg
     5401 accgtggagc gccgaaaccg tcgtctcact cgtcgatctc gttgccgcga cagcgccgga
     5461 gtgcgtgctg atctcgaacc tgcgcacggg acacctgtgg gggtcgcgtc cgtcggcgcg
     5521 gctcctgctc gactacctgc tcgggcgtcc ggtggaggcg ccgcctccgg actgggattg
     5581 cgggcacttc ctgacgatcg cggcgcgtat cggcggtccg ggcggcgccc tcgtcgtcct
     5641 gcgcgacact tacccgcaac tcggccggga cggctaccac ctgcagccgg ccggggcggt
     5701 ggcggcggcc ctcgaacggg gcgacgggca cgaaggcggc gtgctctgcg cctgcgagac
     5761 accggcggcc ggagccctgg ccggacggtt ggggtcggag ggattcgagc tgcgccactg
     5821 ggacaacggc tcgcccgaca ggggcggtga gaccagtgcg gggtgagggt accgcagacc
     5881 tcatccacgg ctcggccctg aacaggcgtg gtcggccccg gtctcggagc gcctcctgaa
     5941 ctgctatctg agcgtcaggc gggccggggt cctccttgag agaccggagc cgaaagaggc
     6001 ccgcgagcgg tacccgcgtg tctgctgacc tgctccggac gctcctcggc gccctggaag
     6061 aggaacttcc gcgggccgtc aggctgcgcg agcggctgca cgccatcccg gagccgagcc
     6121 atgaggagca cgccaccgcc cgcctcgtcg cggaagccct cgggaccgga gtcgtgaagc
     6181 gcgtcgcccg cacgggcttg atggcgcacg ccggaccgcc gggagaggcc gtcgcggtgc
     6241 gggccgagct cgacgcgctg cccatcgagg aggcatcggg cgtgcccttc gccgccacga
     6301 acgggttcat gcacgcctgc ggccacgacg tccacatggc cgccctcgcc gccctgtttt
     6361 gggctgccgg ccgggtcggg gggtccctgc cgaagcccct cgcagccctc taccagccga
     6421 gcgaggaggc ttacccttcg ggggcggacc tcgtcgtcag ggaaggggct ctggcgaggg
     6481 ggacggctgc ggtcgtggcg gcccacgtcc acccggaggt cccgtggggc tcggtgagcg
     6541 tcgaggccgg tccggtgaac gcctcctcgg acaacctacg catcatcgtc gagggggccg
     6601 gtggacacgg ggcgtatcct caccgcgcgc gtgaccccgt gctcgcgctc tccagcataa
     6661 tcgtcgcgct gcagtcactg gtgagccgcc ggctggaccc ggcgcacgcc gcggtcttca
     6721 gcgtcggctg gctgcgcgcg ggatcggcgg agaacgtcat cccgaacgcc gccgaagcgc
     6781 gaggcacgct gcgcgcgctg gaccccggag atcgcgcacc gctccgggag atggcccgcg
     6841 agatcgccgc gcacacggcc cgggcgcacg gctgcgccgc cagcgtggag gtaaccgagg
     6901 gagagcccgc gacggtcaac gacccggccc tcgccgaggc ggcgcgctcg ctgctccccg
     6961 aggcgggctt cgagctggcg ccggcgatga ggtcctgcgg ttcggacgac ttcggattct
     7021 acggccgggt gtcgccgacg ctcatgatgt tcgtggggct aaagagcggc ccggacaccc
     7081 cggacgtgcc gctccaccac ccgcggttcc tgccgcccga tgaagcggtc cctgcggtcg
     7141 cccgcgccca ggcggtcgcc ttcgcggccg ccgcttccct tgacccggcg ccttgactcc
     7201 gcgtacgatc catcaatgag gctggcttag agggaggtat acgtgatgag tccgttcata
     7261 gggaaagagc cggcctagag cgcatggtgg gcaggctcga gggtagggcg gccgtcgtca
     7321 ccggcggcgc tcgcggcata ggacgcggca tcgtggaggc gttcgccgcc gagggcgctg
     7381 acgtcgccgt ggccgacacg ctcccggaac gcgaggccgc ggccgtgctg gcggaagtgg
     7441 agcgcgcggg aaggcgtggc gtctacgtcc acacggacgt ggcggaggag taccaggtac
     7501 ggaacatggt cgacacggtt ctgggagcct tcgggaggat cgacgtcctg gtcaacaacg
     7561 cgggcgtcct aagtcaggtg tatgtggagg agatgccggt cgaggagtgg gacagggtgc
     7621 tgagggtgaa cttgcgcggc acgttcctgt gctgccgcta cgtgttgccg cacatgctgg
     7681 agcgggggga aggacggatc ctcaacatag cctcccagat cgggcagtcg ggggcgccgg
     7741 ggcttgccca ctacagcgcg agcaagggcg gggtcatagc cttcactaaa gccctcgcgc
     7801 gcgaggtcgc cgcgcggggc gtgctggtca acgctattgc tccaggacct atttcgacgg
     7861 gcatcatgcc ttcccagagc gaggaactgg aggaggcccg gaaggccgcg ctcccgatcc
     7921 ggcgcttcgg cacggtggat gaggtcgcgc ccacggccgt cttcctggcc tccggcgact
     7981 cctcctacta cgtcggacag accctcgggc caaacggcgg ggacgtgatg ttttgaacgg
     8041 aggtggcgca tggaggcggt agacctcacg cagatcccgg tcgtggacaa ccactgccac
     8101 gggataagac cggaccagac ctttgagtgc ctcgcgagct ggcggcgggc cttcacggag
     8161 tccacggacc ccgggatgcc gagggatcac gtcgccacca cggtcttcta ccgccgcctg
     8221 atccagacgc tcgccagctt cctcggctgc gagcccgatg aggaagccgt gctcgccgcc
     8281 cgcaacggaa tagaagggcg aaagctcaca ggcgatctcc tgcgcgccgc gaacgtcgat
     8341 accctcctac tcgacacggg cttcccgccg cccgaggagg tgtttcccgt accagagctt
     8401 ggagagctcg gaggctgccg ggcggagccg atgctgcggc tcgaggtcct gatggagagc
     8461 ctgctctcgg aacacaactc tctcgccggg acggaggagg cgctcgcggc cgccctggaa
     8521 gacgtgcgag gcggaggcta cgtggcgctc aagagcatcg ccgcctaccg gacgggactg
     8581 gatatccggg agtggacgag agaggaggcg gaggcatctt tcgacgagta ccgccgggcc
     8641 gccggagagg gctcggcgcg cctggtacac aagccgctcc ttgacactct gctccacgtc
     8701 gcgttcgctg aggcggcccg ccaggaggtc ccggtccagt tccacgccgg ctacggcgac
     8761 gccgacaccg acctgttgct cggcaacccg ctctacctgc gccctgtgct ccagcgcccg
     8821 gactaccgcg gcatgcccgt cgtgctgctg cacgagtgct acccgtacac tcgccagggc
     8881 ggttatctgg ccgccgtcta cgagaacgtc tacctggacc tctcctacgg catcccgctg
     8941 ctcggctaca gcgagatgct ctccttcacg cgggccgcgc tcggcgtcgc cccggcatcg
     9001 aagctcatgt acagctccga cggcataggg gttccggaac tgcactggat gagcgccgca
     9061 gacggccggc gagtgctcgg cgaggcgctg ggcgagctgg tggccaatgg tgagttgggt
     9121 ctccctgaag ccgaggccgc cggcgagggt gtgctgcgcg ccaacgcggc gcgcctgtac
     9181 cggctctagc gcgcggcgga ctctgtccac tccgcctctt cgctgcgttc acccgcgcat
     9241 gccgcaagat cgaagggaat cgactgtacg ggggtttcag ggacgaccag gcccggtcgc
     9301 cgacccgcgg gacgcctgcg gtcaagccgg gcggtggggc tcgccagtga gaacgatatc
     9361 gatctcaaag ggctcggtct gagaaacatg ggccttggcg cggagccggg cgtgggcggg
     9421 agcgagtgcg aggcgcagga cagcatgcac ttcatgggtc agggcatcgc catcccggag
     9481 atggctgact ccgcgaccgg aaggtccata gtgcccgtca ggggcgctat tggggagctg
     9541 gtttacacgg ctattcatcg cgaatgcacg cccctgatcc gacccaggat gaacgacata
     9601 atagaggtgg tggacgatgc gccttctcgt gcggcgggac gaggaagtcc gctcccgcat
     9661 cggtgtcccg gcgtgaaccg cagccggtca cgaaccgtcc cttcggggcg agagggggta
     9721 aaatatgatc tgacctctcg ttcagttggg gacctggact tgagcgagca tgacaagcgt
     9781 caggggtgat gccgtatgaa agatatcttg ccgagaacgg gcttccggct ggagctcgat
     9841 ggccggtcac tgaagataga agacgtggtg gcttttgccc gcggcagggg ggatgcggaa
     9901 tgcattctat ccgctgagtc tatggagaag atcgaggctt cgtgcgcctt gaagcaagac
     9961 ctgatctcgc gtgggatccc gatctacggc gtaacgaccg gcttcgggga cagcgcgaac
    10021 cgccagatct ctcca
//
LOCUS       DASSMB010000108         5173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1543455, whole genome shotgun sequence.
ACCESSION   DASSMB010000108 DASSMB010000000
VERSION     DASSMB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543455"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFJ72_06885"
     CDS             complement(<1..579)
                     /locus_tag="VFJ72_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7479225.1"
                     /translation="MQAVMETYKRLNIAPTSGKGSWLFDDAGNRYLDFIGGIATNSLG
                     HAHPALVKAIQEQAEKLIHCSNLYEVPIQGEVAELLTEATDFDRVFFCNSGTESVEAA
                     IKLARKHAHTTHGPQKHEILTFSNSFHGRTYGGLSATAQPAMQEGFGPMLPGIVHAPY
                     GDLEAAGQLSGPQTAAILVEPIQGEGGVNVPPE"
     gene            complement(567..1322)
                     /gene="argB"
                     /locus_tag="VFJ72_06890"
     CDS             complement(567..1322)
                     /gene="argB"
                     /locus_tag="VFJ72_06890"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET7479226.1"
                     /translation="MVKIGGGSLAGGALEDLPKVLAGGHPVVLVHGGGQQLTRMLDAL
                     GIPTRFHEGLRVTDEATLEVAEMVYAGGVNKSLARELNDLGVPAVGISGTDGPTLVVE
                     PVPGLGRVGSVTEVRPDLIRSLWEGGFAPVVAPLGLGPEGAYNVNADSAAAALAVGLG
                     AGHLLLLTDVDGLLEEGALVRSLAPEECEGYVESGLAAGGMVPKLRAAAEAARGGVEV
                     RILNGNKKGALARAISGEPVGTLISEGVVTCRQ"
     gene            complement(1343..2563)
                     /gene="argJ"
                     /locus_tag="VFJ72_06895"
     CDS             complement(1343..2563)
                     /gene="argJ"
                     /locus_tag="VFJ72_06895"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET7479227.1"
                     /translation="MNKDTTIGIKTLPGGATAPEDFLAGGAACGVGKSGNDLGVLYSE
                     RECVAAAVFTRNVLKGAPLGVTREAVESGGVRAVVANSGNANAATGARGVENARGMQR
                     ISAGALKIPASQVAVASTGVIGVHLPMDRIESGILEAAAGLGRDGSGFAEAILTTDTR
                     IKEAAVEVEIGGKTVTVGGTSKGSGMIHPNMGTMLAFLTTDAAVDKDCLQETLRRATE
                     RTFNRVTVDGDTSPSDMALLLANGAAGNETLTLDSPEYPAFAEAVETVARTLAKEIAR
                     DGEGATRLVEVVVEGAKDEESAAALAKSVVGSSLVKAAVFGEDANWGRVLTAMGYSGV
                     VFDPDGVDLWFGPVQVFAAGEPAEHEEAEANAALSGKEVVITARLSEGDASAVAWGCD
                     LTYKYVEINGSYRT"
     gene            2656..3666
                     /gene="argC"
                     /locus_tag="VFJ72_06900"
     CDS             2656..3666
                     /gene="argC"
                     /locus_tag="VFJ72_06900"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7479228.1"
                     /translation="MALQVAIYGGSGYAGAELIRLLSGHPKVGALGVASRGYAGRAIG
                     EVFPQIAADGEFVAPDEIDASALDVAFVAYGHGESAETVKELLDAGVELVVDLSADFR
                     LPDVDLYREWYGEHPAPELFSEAHYGLPEVFGAAEGRVIANPGCYPTAAILALAPVVR
                     RMDAGIRSVTVNALSGVSGAGAKPSVKTHFVSANENANAYGAPRHRHTPEIEVMLRRV
                     GESPAVTFVPHLIPITRGELETIVVDLDDVPDAGEVLGWYAEDYEDWAFVEARREYPH
                     ITHVANTNRCRLSAAVDERAGKLLLFSAVDNLLKGASGAAVQNMNLALGYPEDLGLEH
                     LK"
     gene            3694..4173
                     /locus_tag="VFJ72_06905"
     CDS             3694..4173
                     /locus_tag="VFJ72_06905"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479229.1"
                     /translation="MGATLSSADKKTRQDLILRLISERDLGTQQDVVAALADEGVEVA
                     QATVSRDLAELGVIKVGNRYLALPHEPGSAGIEVLPSFVLGLTQAQNQVVIHTRDGTA
                     GAVSSVLDRVRGLKILGTIAGQDTVLAIAADNAAAGEVTSLITDVCRARTRPAGNIE"
     gene            4182..4592
                     /locus_tag="VFJ72_06910"
     CDS             4182..4592
                     /locus_tag="VFJ72_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET7479230.1"
                     /translation="MEVRKTCPVPDRALDLTVLEGRLAICRLAAGSEIPAWATTAPFF
                     SVTRTDDEVSVVCPEEMVPSGTRCEQGWRALKLEGPFEFSLVGVLSSVVDPLAEAGIS
                     IFAVSTFDTDYVLVKEDRLEPAIAALRGRGHEVR"
     gene            4616..>5173
                     /locus_tag="VFJ72_06915"
     CDS             4616..>5173
                     /locus_tag="VFJ72_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HET7479231.1"
                     /translation="MTLQGKKVVLAYSGGLDTSVCVKWLEEKGAIPYALYLDLGQGEP
                     AEDVKAKAIQIGAADAFVRDAKTEFVEEYVASAIKANALYGGKYPLFTALGRPLIAKK
                     LVEAAREVGATHIAHGSTGKGNDQVRFDVTTASIAPDLTVVAPVRDWNMSRPEEMAYA
                     EEHGIPVPTTKKSPYSVDENLFGRSI"
BASE COUNT          824 a         1741 c         1723 g          885 t
ORIGIN      
        1 ttccggcggc acgttgacgc cgccctcgcc ctggatcggc tcgaccagga tcgccgccgt
       61 ctgtggaccg gacagttgtc cggctgcttc cagatccccg tagggggcgt ggacgatgcc
      121 gggcagcatg ggaccgaagc cttcctgcat ggcgggctgg gcggtggcgc tgaggccgcc
      181 gtaggtgcgg ccgtggaagg agttggagaa ggtcaggatc tcgtgcttct gtgggccgtg
      241 ggtcgtgtgg gcgtgcttgc gggctagctt gatcgcagcc tcgacggact cggtgccgga
      301 gttgcagaag aagacccggt cgaagtccgt ggcctcggtg aggagttcgg cgacctcgcc
      361 ctggatcggg acttcgtaga ggttggagca gtggatgagt ttctcggcct gttcctggat
      421 ggctttcact agcgccgggt gggcgtggcc cagggagttg gtggcgatgc ccccgataaa
      481 gtccaggtag cggttgcccg cgtcgtcgaa gagccagctc cctttcccgc tcgtcggggc
      541 tatattcaac cgcttgtaag tctccattac tgcctgcatg tcacaactcc ttcggatata
      601 agggtcccga cgggctcgcc cgagatcgca cgggcgagcg cgcccttctt gttgccgttg
      661 aggatcctga cctccacccc gccgcgagcc gcctccgccg ccgcccgcag cttggggacc
      721 attccccccg ccgccagccc gctctccacg tacccctcgc actcttccgg cgcgagcgag
      781 cggaccagcg cgccctcctc cagcaagccg tccacatcgg tgagcaggag caggtgtccg
      841 gcccccaggc ctaccgcgag cgccgccgcc gccgagtcgg cgttgacatt gtacgcgccc
      901 tccggcccga gcccgagcgg ggccacgacc ggcgcgaagc caccctccca cagcgaccgg
      961 ataaggtccg gcctcacctc cgtcacgcta ccgacccgcc cgagtcccgg caccggctcc
     1021 acgacgagcg tcgggccgtc ggtgccggag atgcccaccg ccggcacgcc gaggtcgttc
     1081 agctcgcgcg cgagggactt gtttacgccc cccgcgtaga ccatctcggc gacctccagg
     1141 gtggcctcgt cggttacgcg caggccctcg tggaagcggg tggggatgcc gagggcgtcg
     1201 agcatccggg tgagctgctg tccgccaccg tggacgagca cgaccgggtg gccgcccgcc
     1261 agcactttcg gcaggtcttc cagcgcgcct ccggcgaggg agccgccgcc tatcttcacc
     1321 acgattggct tcgtctccgg catcacgtgc ggtaactgcc gttgatctcg acgtacttgt
     1381 aggtcaggtc gcagccccat gcgaccgccg acgcatctcc ctcggagagc cgggccgtga
     1441 tgacgacctc cttgcccgag agcgccgcgt tcgcctcggc ctcctcgtgc tcggcgggct
     1501 ctccggccgc gaagacctgc accggcccga accacaggtc cacaccgtcc gggtcgaata
     1561 cgactcccga gtagcccatc gccgtcagca cgcggcccca gtttgcgtcc tcgccgaaca
     1621 cggccgcctt gacgaggctg ctcccgacca cgctcttggc tagcgccgct gcggactcct
     1681 catccttcgc cccttccacg acgacttcca ccagccgcgt cgcgccctcg ccatcacgcg
     1741 cgatctcctt tgccagagtc cgtgcaacgg tctcgaccgc ttccgcgaat gccggatact
     1801 ccggcgagtc gagcgtgagc gtctcgttgc cggcggcgcc gttcgcgagc aggagcgcca
     1861 tgtcgctggg agaggtatcg ccgtccacgg ttacgcggtt gaaggtgcgt tccgtggccc
     1921 ggcgaagtgt ctcttgcagg cagtccttat ccacggcggc gtcggtggtc aggaaggcga
     1981 gcatcgtgcc catgttcggg tggatcatgc cgcttccctt cgacgtcccg ccgacggtca
     2041 cggtcttccc gccgatctcc acctcgaccg cggcttcctt tattcgggtg tcggtggtca
     2101 ggatggcctc cgcgaaaccg gaaccgtcgc gccccagacc ggcagccgct tccaggatgc
     2161 cggactctat gcggtccatt gggaggtgaa ccccgatcac acccgtagaa gccaccgcta
     2221 cctgactcgc cggtatcttc aacgccccgg ccgagatccg ctgcatcccc cgcgcattct
     2281 cgacaccccg cgcgccggtc gccgcgttgg cgttcccgct attagccacc accgcccgca
     2341 cgccgccgga ctcgaccgcc tcccgcgtaa cgccgagcgg cgctcccttc aacacgttcc
     2401 gcgtgaacac cgccgccgca acgcactccc gctccgagta cagtaccccg aggtcgttcc
     2461 cggacttccc gaccccgcag gccgcgccac ccgccagaaa atcctccggg gccgtagccc
     2521 cgcccggcag agtctttata ccgattgttg tatctttatt catcaggagt tcgtattgtt
     2581 gcacccgtct gagggagcgt caaccgtttt attcagaaac ttgaataaat atccgtcagg
     2641 tgctctaata cgcgcatggc tctccaggta gcgatctatg gcggcagtgg ttacgcgggg
     2701 gcggagttga ttcgcctgct ctccgggcat ccgaaggtgg gtgcgctggg agtcgcttca
     2761 cggggctacg ccgggcgggc tatcggggag gtcttcccgc agattgcggc ggacggagag
     2821 ttcgtcgcgc cggacgagat cgacgcctcg gcgctcgacg tggcgttcgt ggcctacggt
     2881 cacggcgaga gcgcggagac ggtaaaggag ttgctggacg ccggggttga gctggtcgtg
     2941 gacctctcgg cggacttccg gctgccggat gtggatctgt atagagagtg gtacggggag
     3001 catcccgcgc cggagttatt cagcgaggcg cactacgggc ttcccgaggt cttcggggcg
     3061 gcggagggcc gggttatcgc caatccgggg tgctatccga cggcggctat cctggctctc
     3121 gcgccggtgg tgcggcggat ggacgcgggg atacgctccg tgaccgtgaa cgcgctctcc
     3181 ggggtgagcg gggcgggggc gaagccgagc gtgaagacgc acttcgtgag cgccaacgag
     3241 aacgccaacg cctacggggc gccgcgtcac cggcatacgc cggagataga ggtcatgctg
     3301 cggcgggtgg gggagagccc ggcggtcacg ttcgtgccgc acctcatccc gatcacgcgc
     3361 ggagagctcg agaccatcgt ggtggacctg gacgacgtac cggatgcggg ggaggtcctc
     3421 gggtggtacg cggaggacta cgaggactgg gccttcgtcg aggcgcgccg ggagtatccg
     3481 cacatcacgc acgttgcgaa cacgaaccgc tgccggctct cggcggcggt ggacgagcgg
     3541 gccgggaagc tgctcttgtt ctcggcggtg gacaacctgc tcaagggcgc gtcgggagcg
     3601 gcggtgcaga acatgaacct ggcgctcggt tatcccgagg acctcgggct agagcatctc
     3661 aaataagagt tataggaata taggaggcgg gaattgggcg cgacactatc atcggcggac
     3721 aagaagacca ggcaggatct gatcctgcgc ctgatctccg agcgtgacct cggtacccag
     3781 caggacgtgg tcgccgccct ggccgacgag ggcgttgagg tcgcccaggc caccgtgagc
     3841 cgcgacctcg cggaactcgg cgtcataaag gtcggcaacc gctacctggc gctgccgcac
     3901 gagcccggtt ccgccggcat cgaggtcttg ccgagcttcg tactcgggct gacccaggcc
     3961 cagaaccagg tcgtcatcca cacccgcgac ggtaccgcgg gcgccgtctc cagcgtcctc
     4021 gaccgggtgc gcgggctcaa gatcctcggc accatcgccg ggcaggatac cgtgctcgcc
     4081 atcgccgccg acaacgccgc cgccggagaa gttacgagcc tcatcaccga cgtctgccgc
     4141 gcaaggaccc gtcccgccgg caacatcgag tagtaattag cgtggaggtt cggaagacgt
     4201 gcccggtacc ggaccgtgcg ctcgatttga ccgtgttgga ggggcgtctg gcaatttgcc
     4261 gcctggcggc tgggtcggag ataccggcgt gggctaccac agcgccgttc ttctccgtta
     4321 cccgcactga cgatgaagtc tccgtcgtct gcccggagga gatggtgccg tccggtacgc
     4381 ggtgcgagca gggctggcgc gccctgaagc tggaggggcc gttcgagttc tcactcgtag
     4441 gcgtgctatc gtcggtggtt gacccgctcg cggaggccgg tatcagcatc ttcgccgtct
     4501 ccaccttcga tacggactat gtgctcgtca aggaagaccg actcgaaccc gccatcgccg
     4561 cgttgcgtgg gcggggacac gaggttcgat gatctctgat actcaaggag gaaagatgac
     4621 tctgcaaggc aagaaagtcg tgctcgccta ctccggtggg ttggatacat cggtgtgcgt
     4681 gaagtggctg gaggagaagg gggctatccc ttacgcgctg tacctggacc tcgggcaggg
     4741 agagcccgcc gaggacgtga aggccaaggc gatacagata ggagccgccg acgccttcgt
     4801 gcgggacgcg aagaccgagt tcgtggagga gtacgtcgcc tcggcgataa aggcgaacgc
     4861 gctctacggc ggcaagtacc cgctcttcac ggctctcgga cggccgctca tcgcgaagaa
     4921 gctggtggag gccgcgcgtg aggtgggagc gacgcacatc gcgcacggct cgacggggaa
     4981 gggcaacgat caggtccgct tcgacgtcac gaccgcctcc atcgccccgg acctcaccgt
     5041 cgtcgccccc gtccgcgact ggaacatgag ccgcccggag gagatggcct acgccgagga
     5101 gcacggcatc cccgtcccga ccaccaagaa atccccctac agcgtggacg agaacctctt
     5161 cggccgctcc ata
//
LOCUS       DASSMB010000109         3031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_529568, whole genome shotgun sequence.
ACCESSION   DASSMB010000109 DASSMB010000000
VERSION     DASSMB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529568"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFJ72_06920"
     CDS             <1..517
                     /locus_tag="VFJ72_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479232.1"
                     /translation="TGGSYWTTFFPAVVVQGLGMALVIAPLTTTAMNSVTGRHSGLAS
                     GVNNAVSRTAGLLAIPVLGILVFLVFSAGLDSRVAALDLPPQAKQQLETEKVDLGGAK
                     VPDGVSTATASQIERAVKESFVAAFRLAMYAGAGLALASALAAALIIEGKGQTAPAGR
                     VGQPGSEGAPA"
     gene            536..802
                     /locus_tag="VFJ72_06925"
     CDS             536..802
                     /locus_tag="VFJ72_06925"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET7479233.1"
                     /translation="MQQCTHLDQIKDVEPSAEGCEDCLRMGDTWVHLRECLTCGHVGC
                     CDSSKNKHATKHFHATDHPLIQSFQPGEDWIWCYVDEVVMEPRR"
     gene            825..1124
                     /locus_tag="VFJ72_06930"
     CDS             825..1124
                     /locus_tag="VFJ72_06930"
                     /inference="COORDINATES: protein motif:HMM:NF023070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479234.1"
                     /translation="MIVLVDLKEGVSPEDYERWVRETYRPAVMTLSSIERWRGYRASA
                     LLGSEAAPPHQYVVVVEIEDLEQLGRDMTDETLQALLSQLHAFADVTQIMADRFA"
     gene            1196..2767
                     /locus_tag="VFJ72_06935"
     CDS             1196..2767
                     /locus_tag="VFJ72_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET7479235.1"
                     /translation="MAAVAILATLADRIRVPYPILLVIGGLAMGFVPGLPKVELEPDV
                     VFLLFLPPLLYVSALFTSWRDFRANLRPISLLAIGLVLMTTCVVAAVAHWVVGLPWAA
                     AFVLGAIVSPTDAIAATAVAQRLGVPRRIVVVLEGESLVNDATGIVAYRIAVTAVAAG
                     AFSLWEAGLQFVVGAVGGVAVGLAVGWIIIWARRHVSENASVQNTISLLSPFAAYLLA
                     EEPSHYAWQALGLPGDFAFSGVLSVVATGLYLGRRVSFSVSAETRLQGFAFWELATFL
                     LNGLIFALIGLQLRRIIEGISDYSVAELVIYAGLISLTVILVRFLWVFPATYVPRWAS
                     PRLRERDPSPPWQAVAVISWTGMRGVISLAAALALPLSVPGRDLILFLTFSVILATLV
                     VQGLSLPALIRGLGLEDDGSTQREEVTGRIEVAQAALDRLEELVEEEWVREETAERVR
                     GLYNYRRGRFAARFDGDSEGVEERSTAYQRLMIELLRAQRRTLIRLRNEGEISDEAMH
                     RIERDLDLEESRLEI"
     gene            2775..>3031
                     /locus_tag="VFJ72_06940"
     CDS             2775..>3031
                     /locus_tag="VFJ72_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7479236.1"
                     /translation="MSDIEFLILLLGAVAVLAQLARITNVPYPIFLVLGGLAIGLAPG
                     LPKIELAPEVIFLVFLPPLLNSAAFTASPRDLRAHLRPIAML"
BASE COUNT          458 a         1009 c         1009 g          555 t
ORIGIN      
        1 caccgggggc tcctactgga cgaccttctt cccggccgtc gtggtgcagg gcctcggcat
       61 ggcgctcgtc atagcgcccc tcacgaccac ggccatgaac tccgtcacgg gccgccattc
      121 cgggctggcc tcgggcgtca ataacgccgt ctcccggacg gccggcctgc tcgccatacc
      181 cgtgctcggt atcttagtct tcctcgtctt ctccgccggg ctggacagcc gcgtggcggc
      241 gctggatctc ccgccgcaag ccaaacaaca actcgaaacg gagaaagttg acctcggcgg
      301 cgcgaaggtc ccggacggcg tctcgacggc gaccgcgtca caaatagaga gggccgtgaa
      361 agagtcgttc gtggcggcgt tccggctggc gatgtacgct ggggcgggac tcgcgctcgc
      421 cagcgccctc gccgcggcgc tcatcataga aggcaaggga cagacggcgc ccgcaggcag
      481 ggtcggacag cccggcagcg agggcgctcc tgcctgatcc gagaggagaa accctatgca
      541 acaatgcacg cacctggacc agatcaaaga cgtcgagccg agcgccgagg ggtgcgagga
      601 ctgcctgcgg atgggcgata cctgggtcca cctgcgcgag tgcctcacct gcggccacgt
      661 cggctgctgc gacagctcga agaacaaaca cgccacgaag cacttccatg ccacggatca
      721 cccgctgatc cagtcgttcc agccgggaga agactggatc tggtgctacg tagatgaagt
      781 cgtgatggag ccgcgccgtt agagaggaac ccatcgtgcc gacgatgata gtgctggtgg
      841 acctgaaaga gggcgttagt cccgaggact acgagcggtg ggtccgcgaa acctaccgtc
      901 cggcggtgat gaccctctcc tccatcgagc gctggcgggg ttacagagca agcgcattgc
      961 tcggctcgga agctgctccg ccccatcagt acgtcgtagt cgtcgagata gaggatctgg
     1021 agcaactcgg cagagacatg acggacgaga cacttcaggc attgctctcg cagttgcacg
     1081 ccttcgcgga tgtcacccag atcatggcgg acaggttcgc gtaggatagg gttgttcgca
     1141 ccggtataga aagagaggcg gacctctgcc tgagatagag gcgatcctga ttctgctggc
     1201 cgcggttgcg atactagcga cgctcgcgga ccggatacgg gtcccgtacc cgatcctgct
     1261 cgtgatcggc ggtctcgcga tgggcttcgt gccggggctg ccgaaggtgg agcttgagcc
     1321 tgacgtggtg ttcctgctct tcctgccacc tctgctctac gtctcggcgc tctttacctc
     1381 gtggcgggac ttccgggcga acctgcggcc gatctcgctg ctcgccatcg ggctggtcct
     1441 tatgacgacc tgcgtggtgg cggcggtggc ccactgggta gtcggtctgc cctgggccgc
     1501 ggccttcgtc ctcggggcca tcgtctcccc cacggacgcc atagccgcca cggccgtcgc
     1561 ccagcgtctc ggcgtacccc gccgcatagt cgtcgtgctg gagggcgaga gcctggtcaa
     1621 cgacgccacg ggcatagtcg cctatcggat agcggtaacc gctgtcgccg ccggcgcgtt
     1681 ctcgctgtgg gaggcggggt tgcagttcgt cgtgggggcc gtcggcggcg tggccgtggg
     1741 gctggcggtc gggtggatca ttatctgggc ccggcggcac gtctcggaga acgcctccgt
     1801 gcagaacacc atctcgttgc tctcaccctt cgcggcatac cttctggcgg aggaaccctc
     1861 ccactacgcc tggcaggccc tgggcctgcc gggagacttt gctttctccg gggttctctc
     1921 ggtggtggct acggggctgt acctggggcg tcgcgtctcg ttcagcgtgt cggcggagac
     1981 gcggttgcag ggattcgcgt tctgggagct ggcgacgttc ctccttaacg gcctgatctt
     2041 cgccctgatc ggactgcaac tccgccgcat catcgagggc atttccgact actcggtcgc
     2101 cgagctggtg atctacgccg gattgatcag cctgaccgtg atactggtgc gtttcctatg
     2161 ggtcttcccg gcgacctacg tgccgcgctg ggcgagccct cgcctgcgcg agcgcgaccc
     2221 ctcgccgccc tggcaggccg tcgcggtcat ctcctggacg gggatgcgcg gcgttatctc
     2281 gctcgcggcg gcgctcgcgt tgcccctgtc ggtccccggc cgcgatctca tactcttcct
     2341 caccttctcc gtcatcctgg cgacgctcgt ggtgcagggg ctctcgctgc cggccctgat
     2401 ccggggtctc ggcctcgaag atgacggcag cacgcagcgg gaagaggtaa ctggcaggat
     2461 cgaggtcgcc caggccgccc tggaccggct ggaggagttg gtcgaagagg agtgggtgcg
     2521 cgaggagacg gcggagcgcg tgcgcggtct ctacaactac cgccggggac ggttcgccgc
     2581 ccgcttcgac ggcgactcgg aaggtgtcga ggagcgctcc accgcctacc agcggctcat
     2641 gatcgagctg ctccgcgccc agcgccgcac cctcatccgc ctgcgcaacg agggtgagat
     2701 cagcgacgag gccatgcacc gcatcgaacg cgacctggac ctggaagagt cccggctgga
     2761 gatatgaggg ccggatgagc gacatcgagt tcctgatcct gctgctcggc gccgtggcgg
     2821 tcctcgcgca gctcgcgcgt attacgaacg tcccctaccc catcttcctg gtcctcggcg
     2881 ggctggcgat cgggctcgcg ccggggctcc cgaagatcga gctggcgccg gaggtgatct
     2941 tcctggtctt cctcccccct ctcctcaact ctgctgcctt cacagcctcg ccccgcgact
     3001 tgcgcgcgca cctgcgcccc atcgcgatgc t
//
LOCUS       DASSMB010000110         4408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_908788, whole genome shotgun sequence.
ACCESSION   DASSMB010000110 DASSMB010000000
VERSION     DASSMB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908788"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1571
                     /locus_tag="VFJ72_06945"
     CDS             114..1571
                     /locus_tag="VFJ72_06945"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7479237.1"
                     /translation="MYTRTRDYPMFVGGKWAAGESGARMEATSPATGEKIGTAPEGTR
                     EDVRHAIAAANEAWPAWAARSAFERAAAMVRISSIIEERRDDLAWTLTLDQGKPLAAE
                     AYDEVDELIAYFEMASADATRLDGSMPPSVDAGKRVFLYRVPRGVVGVISPWNWPYTM
                     PAEAIAPALASGNAVVWNPASSTSICAVKLAECIVDAGLPPGVFNMITGPGSVVGDEV
                     AASPGTQAVAFIGSVETGRKVAARAAGKALLLEMGGNGPMVVLDDADLRAAANASLTA
                     AFLGAGQSCTAGERFLVHQTVHDQFLEELNAAIEREIRLGDPFDPSTTLGPLNNEPTA
                     GKMDRHISDALDRGAELVGGGARADGFPTRLYYQPTVIDGVTGEMQVAREETFGPIVP
                     ITTIRDESDALRAVNSSPYGLLSAVWTGDLARGLRFAEAVRTGWVNINASTNHWESHL
                     PFGGRAGSHSGVGRVGGRYPMETFTELKTVIVSLE"
     gene            complement(1558..2385)
                     /locus_tag="VFJ72_06950"
     CDS             complement(1558..2385)
                     /locus_tag="VFJ72_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7479238.1"
                     /translation="MEKSDEGSGTTRTLSRTGTFVQSVDRAISVMELLARQGWSGVTE
                     VAAEMDIHKSTAYRLLSTLKERGLVEQDAVTEKYRLGFGMVLLASAVTEDIDIVRHAR
                     PVCERLSKQLEETVTVAVLEGDEAVIIHQTMPSTNSVLNVDWSGQHTPLHCTADGKVL
                     LAHLPNERRQRILRRKLERYTENTIVDAAILREQLKKVLDDGYSYTIEELEIGLNGVG
                     APIFGSDGQVVAAISVSGPAFRMSERSIPEIGDLARRTTAEISRQLGFHGQGISTPG"
     gene            complement(2529..3785)
                     /gene="solA"
                     /locus_tag="VFJ72_06955"
     CDS             complement(2529..3785)
                     /gene="solA"
                     /locus_tag="VFJ72_06955"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET7479239.1"
                     /translation="MAHGSQSHRYDAIVVGAGAMGSAAAYYLARRGKRVLCLERFGIP
                     HSMGSSHGHTRIIRLAYYEHPSYVMLLRRAYELWREIQETAGEKLLHITGSIDSGPAE
                     SWVFKGSWESCKLHDLPHEVVTGAELRRRHPGYHLPPEHLALLQPEGGFLTPERCIVS
                     YVVAAQALGAEFHAREKVLEWEPLDGGARVRTEHDTYEADRLVVTAGAWNGGLVDVLD
                     GLAVPERQVLAWLQPTRPEYFRLDTFPVFNLLVDEGRFYGFPVFGVPGFKFGKYHHLE
                     EVVDPDEVDREVHDYDERLLRQFAERYFPDGCGPTMDLQTCMFTNTEDNHFVIDVHPD
                     YEQVSFASPCSGHGFKFASVVGEIMADLAEKGMTRHDISLFRLERLRQPSRRESYRLS
                     HGSQQVREQNGRPGSFPRVSDVKPFW"
     gene            complement(3785..>4408)
                     /locus_tag="VFJ72_06960"
     CDS             complement(3785..>4408)
                     /locus_tag="VFJ72_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET7479240.1"
                     /translation="NLKPYGVLAMQSLRIEKSLPLYGPDISEDDTPFHVGLSRWIHLE
                     KRDFIGREALLRVQERGLDRRWVGLTLESEVPAAAGAKVYSIGDVATFREMMDTGAEA
                     GEYEDAVMPGERQVGRVTSSAMGHSVGKMLAMAYVDTAHSWPGANLIVEINGRLIAAK
                     VSPIPFFDPENARIRSEPQEDDRRSEPASRPLSPASVSANSRTNGKT"
BASE COUNT          716 a         1518 c         1441 g          733 t
ORIGIN      
        1 ccactgctcg cccgcatcgc ctatgaggac ccgcaaacgg tcgggaaccc gcgcgaggtc
       61 acggccgccg actacgaggg gatctaccac aatgccttcg caagggggaa gtgatgtaca
      121 cccggaccag agactacccg atgttcgtcg gcggcaaatg ggccgccggc gagtccggcg
      181 cccgcatgga ggccacgagt ccggcgaccg gcgagaagat cgggaccgcg ccagagggca
      241 ctcgtgaaga cgtgcgccac gcgatagccg ccgcgaacga agcctggccc gcctgggcgg
      301 cgcgctccgc gttcgagcgc gccgcggcga tggtccggat ctcctcgatc atcgaagagc
      361 gccgcgatga cctggcgtgg acgctaacac tcgaccaggg caagccgctg gcggcggaag
      421 cctacgacga ggtggacgag ctgatcgcct acttcgagat ggcgagcgcc gacgccacgc
      481 ggctcgacgg ctcgatgccg ccctccgtgg acgccgggaa gcgggtcttc ctctaccgcg
      541 tcccgcgcgg ggtggtcggt gtgatctcgc cgtggaactg gccctacacg atgcccgccg
      601 aagccatcgc cccggctctc gcctccggga acgccgtcgt ctggaacccc gcctcttcga
      661 cctccatctg cgccgtgaag ctcgcagagt gcatcgtgga cgccggactc ccgcccggag
      721 tcttcaatat gatcaccggc cccggctccg tcgtcggcga tgaggtggcg gcgagccccg
      781 gcacccaggc cgtcgccttt atcggctccg tggagaccgg ccggaaggtc gccgcccgcg
      841 ccgccgggaa ggcgctcctc cttgagatgg gcggcaacgg gccgatggtg gtcctggacg
      901 acgccgacct tcgagccgcc gcgaacgcca gcctcaccgc cgccttcctc ggcgccgggc
      961 agagctgcac cgccggagaa cgcttcctcg tccaccaaac tgtccacgac cagtttctgg
     1021 aagagttgaa cgcggcgata gagcgcgaga taaggctcgg agaccccttc gacccatcca
     1081 cgaccctggg cccgctcaac aacgagccca ccgccgggaa gatggaccgc cacatctccg
     1141 acgccctcga ccggggcgcg gagttggttg gcggcggcgc gcgggcggac ggcttcccga
     1201 cccgcctcta ctaccagccg accgtgatcg acggcgtgac cggcgagatg caggtcgccc
     1261 gcgaggagac cttcggaccc atcgtcccga taactaccat ccgggatgag agcgatgcgc
     1321 tgcgcgccgt caactcctcg ccttacggtc tgctctcggc ggtatggacc ggggacctgg
     1381 cgcggggtct gcgcttcgcc gaggcggtgc ggaccggatg ggtcaacatc aacgcctcca
     1441 cgaaccactg ggagagccac ctccccttcg gcggccgcgc cgggtctcac tcgggcgtcg
     1501 ggcgcgtcgg gggccgttac ccgatggaga ccttcaccga gttgaagacc gtgatcgtca
     1561 gcctggagta gagatgccct gaccgtggaa cccgagttgg cgcgagatct cggcggtcgt
     1621 ccgcctggcg aggtcgccga tctcgggtat cgagcgctcc gacatcctga atgccggacc
     1681 ggagacgctg atggccgcca cgacctgccc atcggagccg aagatcgggg ctccaacccc
     1741 gttcagcccg atctccagtt cctcgatggt gtagctgtag ccgtcgtcca ggaccttctt
     1801 caactgctcc ctcaggatcg cggcgtccac gatggtattc tccgtgtagc gctccagctt
     1861 gcggcggagg atacgctggc gccgctcgtt cggcaggtgg gccagcagca ccttgccgtc
     1921 ggccgtgcag tgtagcgggg tatgctgccc cgaccagtcc acgttaagga cggagttcgt
     1981 tgagggcatg gtctggtgga tgatcacggc ctcatcaccc tccaaaaccg ccacggtcac
     2041 cgtctcttcg agctgcttgc tcaggcgctc gcacaccgga cgggcgtggc gcacgatatc
     2101 tatatcctcc gtgactgcgc tcgcgagcaa caccatcccg aagcccagcc ggtacttctc
     2161 cgtcacggcg tcctgctcca ccagcccccg ctccttgagg gtcgagagca gccggtaggc
     2221 ggttgacttg tggatgtcca tctcggcggc gacctcggtt acgccggacc atccctgccg
     2281 ggcaagcagc tccatcaccg agatcgcccg gtctaccgac tggacgaagg tgcccgtccg
     2341 gctcagagta cgcgtcgtac ctgacccttc atcgctcttc tccacgctca tcccctctcg
     2401 gaaggacgcg accatacctg actcgtcacg gcttgcagcg gcgtatctcg tccccgcttc
     2461 gggaccattc tagagcaaca cggaaattca cggcgcccgc gcccggcccg cgcccaccta
     2521 ccacggcttc accagaaagg ctttacatcc gacactcgcg gaaaggaccc cggccggccg
     2581 ttctgctcgc gaacctgctg gctgccgtgt gacagccggt agctctcgcg gcgcgaaggc
     2641 tgtctgaggc gttcgaggcg gaacaggctg atatcgtggc gggtcatccc cttctcggcg
     2701 aggtcggcca tgatctcccc gaccacgctg gcgaacttga acccgtgccc tgagcacggc
     2761 gaggcaaacg agacctgctc ataatctgga tgcacgtcga tgacgaagtg gttgtcctcc
     2821 gtattcgtga acatgcacgt ctgaaggtcc atcgtcggcc cgcaaccgtc cgggaaatat
     2881 cgttcggcga actgccggag cagccgctcg tcgtagtcgt gtacctcgcg gtccacctcg
     2941 tcggggtcca cgacctcttc gagatggtga tacttgccga acttgaagcc gggcacgccg
     3001 aagaccggga agccgtaaaa gcggccctca tccacgagca ggttgaacac ggggaacgta
     3061 tctagccgga agtactcggg acgggtcggt tgcagccagg cgaggacctg cctctccggg
     3121 acggcaagcc cgtcgagtac gtcaaccagg ccgccgttcc aggccccggc ggtcactacg
     3181 agccggtcgg cctcgtaggt atcgtgttcg gtccggacgc gcgctccgcc atcgagcggc
     3241 tcccattcca ggaccttctc gcgggcgtgg aactcggccc ccagcgcctg ggccgccacg
     3301 acgtaggaga cgatgcagcg ttcgggggtg aggaagccgc cctcgggctg gaggagggcg
     3361 aggtgttcgg gcggcaggtg gtatccgggg tgccgccgcc tcaactccgc gccggtcacg
     3421 acctcgtgcg gcaggtcgtg cagcttgcag gactcccacg atcccttgaa gacccaggat
     3481 tccgcggggc ctgagtctat ggagccggtg atgtggagta gtttctctcc ggccgtctct
     3541 tgaatttcgc gccacagctc gtaggcgcgc cggaggagca tgacgtagga ggggtgctcg
     3601 tagtaggcca gccggattat tctcgtgtgg ccgtgcgagg agcccatgga gtgcgggatg
     3661 ccgaacctct ccaggcagag cacgcgcttg ccccggcggg cgaggtagta ggcggcggcg
     3721 ctccccatcg cgcccgcccc gactacgatg gcgtcgtagc ggtgtgactg cgagccatgg
     3781 gccatcaagt cttcccgttc gtccgggagt tggccgatac gctcgccgga gagagcggcc
     3841 gggaggcggg ctcggagcgc cggtcgtcct cctggggctc ggaccggatg cgggcgttct
     3901 ccgggtcgaa gaacgggatc ggggagactt tcgcggcgat gagacggccg ttgatctcca
     3961 cgatcaggtt cgcccccggc caggaatgag cggtatccac gtaggccatc gccaacatct
     4021 tgccgacgct gtggcccatc gcgctcgacg ttacgcgccc gacctgacgc tctccgggca
     4081 ttacggcgtc ctcgtactct ccagcctcgg ccccggtgtc catcatctcc cggaacgtcg
     4141 ccacgtcgcc gatgctgtag accttcgcgc ccgcggcggc cgggacctcg ctctccaggg
     4201 tcaggccgac ccagcgacgg tccaggccgc gttcctgcac ccgcaggagg gcttcgcgcc
     4261 cgatgaagtc gcgtttttcg aggtggatcc aacgtgatag ccccacgtgg aacggggtgt
     4321 cgtcctcact gatgtccggg ccatagagcg gcaggctctt ctctatccgc agggactgca
     4381 tcgccagcac gccgtagggc ttgaggtt
//
LOCUS       DASSMB010000111         3765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1038316, whole genome shotgun sequence.
ACCESSION   DASSMB010000111 DASSMB010000000
VERSION     DASSMB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038316"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..550)
                     /locus_tag="VFJ72_06965"
     CDS             complement(35..550)
                     /locus_tag="VFJ72_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7479241.1"
                     /translation="MSAALLVLAVAVVAAQRLLELRYSRRNERRLRAKGAVERGASHY
                     PLMVAIHTLWLVSTLVEGLLRGPDVPAWWPVPLAVFLLVQPLRYWAILSLGENWNVRI
                     LVVPGRRLVRRGPYRYFPHPNYVVVAVEVLTLPLIFGAWVTAVVFTALDAAFLFVRIR
                     EEERALREIAG"
     gene            complement(570..1622)
                     /locus_tag="VFJ72_06970"
     CDS             complement(570..1622)
                     /locus_tag="VFJ72_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET7479242.1"
                     /translation="MSRPRPKVLSVATALPPHHLGQGEVKDFARGMFSEVFRDIERLT
                     PVFDNVQVESRYFCVPLEWFERDHTFPEKNALYVEHALSLSEKAARRAMDRAGVEPGE
                     IGAIFFVSTTGLSTPSLDSKLIFALDLSEHTRRVPIWGLGCAAGAAGVSRAAEYATLY
                     PEQKVLLVAVELSGLTFQRGDRSKSNLISTSLFADGAAAVVLGTGAGPEIVGSYSTTW
                     PGTEDVMGWDLIETGLKVQLSKSVPTIVRERLLGNLQDACDSLDLDFADLRHYVLHPG
                     GAKVLDAFEEVLGLAPGALTFSRSVLRDFGNMSSVTVLFILERFLESGEYAPGDLGVL
                     SAMGPGFSAEHVFFKC"
     gene            complement(1619..2419)
                     /locus_tag="VFJ72_06975"
     CDS             complement(1619..2419)
                     /locus_tag="VFJ72_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7479243.1"
                     /translation="MEFLEVLKRRKTTNGPFLPDPVSPEHQRLLVEAAAMAPSHFNSQ
                     PWRFVLVDDRETIEEVARISGESMTRLLEEGTFWKRYRPYFRFSEAEMEEKRDGIYFD
                     QMPSVLKPFSRQIFSNTGQAVMNRFGVPKTLGEDNRKLVAGSPLLLAVLLDKTEYRPG
                     ELSGFYSVFGMGAAVENIWLTTAEIGMGIQFVSTPMEIPENWKRMQDLLEVPDDLELM
                     AVYRLGYVPKEQRRPTIDWSSRQRKRLSQYVFRNTCATPETDAEPGAR"
     gene            complement(2442..2750)
                     /locus_tag="VFJ72_06980"
     CDS             complement(2442..2750)
                     /locus_tag="VFJ72_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479244.1"
                     /translation="MIFAVAAILFLANFALGVLVQLRIVDTKPFRWLHHALFFAVFVS
                     AAVAVVWGFWQSSPYRWALLPALALFAVLPRVRAGTVGHAALASGALILYAAGFVFML
                     "
     gene            complement(2874..>3765)
                     /locus_tag="VFJ72_06985"
     CDS             complement(2874..>3765)
                     /locus_tag="VFJ72_06985"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HET7479245.1"
                     /translation="VPYKFPQKGPHLTLGFHRYALTGGFTSLSEEGLEDVLAWAGTPE
                     VSPEKAPERVPSPASETRIAERPAASGHRTARPVVNDEVLEKRSAGQHAMGKARKQIV
                     LWTIAETLTQAVGWKAAHKNARDKALDAQRTWLKGGRAEKQVGAELERLREHGFYIFH
                     DVQLPDVGNVDHVALGPRGFFGIETKSQSGGVRARGNQLLLNGRPPGKDFVSQTWSGC
                     FRLKDILDAEVSPLLCFTEAFVEGRVFVRGVRVLPLRWLVEEILRGEVMHDSLTVAKA
                     VNALGAATGCYPSSVPRAKS"
BASE COUNT          693 a         1250 c         1199 g          623 t
ORIGIN      
        1 ctgtttcgta gtacctgact gctggcggct aatattatcc cgcaatttcc cgcagcgccc
       61 gctcctcttc tcgtatccga acgaacagga acgcggcgtc gagcgcggtg aagaccacag
      121 ccgtaaccca ggctccaaag atgagcggca acgtcaggac ttcgacggcc acgactacat
      181 agtttggatg cgggaagtac cggtacggac cgcgccggac gagcctccgg ccgggcacga
      241 cgagtatccg cacgttccag ttctcgccca gggagaggat cgcccagtac cgcaggggct
      301 ggacgagcag aaacaccgcg agcggcaccg gccaccacgc cgggacatcc gggccgcgca
      361 agagaccctc gacgagcgtg gagacgagcc agagagtgtg gatggcgacc atcaacgggt
      421 aatgacttgc gccgcgttcg acggctcctt ttgcccgcag acgccgctcg ttgcgccggg
      481 agtaccgcag ttcgaggagg cgctgcgcgg cgacgaccgc tacggcaagg acgagcaacg
      541 ccgcgctcac gggatgctac agccggtagc taacacttga agaagacgtg ctccgcggag
      601 aagcccggac ccatcgccga gaggacgccg aggtcgccgg gcgcgtactc gccgctctcc
      661 aggaaacgct ccaggatgaa cagaacggtc acgctggaca tgttgccgaa atcgcgcagc
      721 accgagcgcg agaaggtcag ggcgccgggc gcgagcccca gcacctcctc gaaagcgtcg
      781 aggaccttcg cgcctccggg gtgcagtacg taatgtctca ggtccgcgaa gtccagatcg
      841 agggagtcgc aggcgtcttg caagttgccg agcagccgct cgcgcacgat ggtcgggacg
      901 ctcttggaga gttgtacctt gaggccggtc tcgatgagat cccagcccat cacgtcctcg
      961 gtcccaggcc aggttgtgct gtagctgccg acgatctcgg gtccggcgcc cgttcctagg
     1021 actacggcgg cggctccgtc ggcgaagagg ctcgttgaga tgaggttgct cttggagcgg
     1081 tcgccgcgct ggaaggtgag gccggagagc tccacggcga ccagcaggac tttctgttcg
     1141 gggtagagcg ttgcgtactc ggcggcgcgg gagactccgg ctgccccggc ggcgcatccg
     1201 aggccccaga tcgggactcg ccgcgtgtgc tcggagagat ccagggcgaa gatcagcttc
     1261 gagtcgagcg agggcgtgga gagcccggtc gtggagacga agaagatcgc tccgatctcg
     1321 cccggctcca cccccgcccg gtccatcgcc cgccgggctg ccttctcgga gaggctcagg
     1381 gcgtgctcga cgtagagcgc attcttctcg gggaaagtgt ggtcgcgctc gaaccactcc
     1441 agcggcacgc agaagtagcg gctctccacc tggacgttgt cgaagacggg ggtcagccgc
     1501 tcgatgtcgc gaaacacttc ggagaacatc ccgcgcgcga agtccttgac ctcgccctgg
     1561 cccaggtggt gtggcggcag ggcggtcgcc acggagagga ccttcgggcg cggccggctc
     1621 atcgggctcc gggctccgca tcggtctcgg gcgtggcgca ggtgttgcgg aagacgtact
     1681 gcgagagtcg cttgcgctgg cgactcgacc agtctatcgt cgggcgccgc tgctccttcg
     1741 ggacgtagcc cagccggtag acggccatca gttccaggtc gtcggggact tcgaggaggt
     1801 cctgcatcct cttccagttc tccgggatct ccatcggcgt cgagacgaac tggatgccca
     1861 tcccgatctc cgccgtcgtc aaccagatgt tctcgaccgc cgcgcccatc ccgaacacgg
     1921 agtagaagcc ggacagctct ccgggccggt actcggtctt gtccagcagg acggccagca
     1981 gtagcgggga accggcgacg agcttgcggt tatcctcacc taaagtcttc gggacgccga
     2041 agcggttcat cacggcctgc ccggtgttcg agaagatctg gcgcgaaaaa ggcttgagta
     2101 cggacggcat ctggtcgaag tatattccgt cgcgcttctc ctccatctcg gcctcggaga
     2161 accggaagta tggccggtag cgcttccaga acgtgccctc ttccaggagc cgggtcatgc
     2221 tctcgccgct gatcctggct acctcctcta tggtctcgcg gtcatcaacg agcacgaagc
     2281 gccagggctg gctgttgaag tgcgacgggg ccatcgcagc cgcctcgacc agcagccgct
     2341 ggtgctccgg gctgaccggg tccggcagga aggggccgtt cgtagtcttg cgcctcttga
     2401 ggacttcgag aaattccaag ctagcgttcc tcctagacct tctagagcat aaatacgaat
     2461 ccggcggcgt agaggatcag ggctccgcta gccaacgccg cgtgccccac agtcccggcc
     2521 cgcaccctcg gcaatacggc gaagagagcc agcgccggca ggagcgccca cctatagggc
     2581 gaactctgcc agaagcccca gacgaccgcc acggccgccg agacgaagac cgcgaagaac
     2641 agggcgtggt gcagccaccg gaaaggcttc gtgtccacga tgcgtagctg gacgagcaca
     2701 cccagcgcga agttagccag gaacaagatc gccgcgacgg cgaagatcaa agcggactcc
     2761 tcatcatcgc tacaggtata gcacagacgc cgccggcaga aggcgtatcc tttctcctat
     2821 ttcgaggcgt ccgtccgcat gagcgcatta ggacgcgaca cgctcccggc tccctaactc
     2881 ttcgcccgcg gcacggacga cggatagcaa ccggtcgcgg cgcccagagc gttgaccgcc
     2941 tttgcgaccg taagggagtc gtgcatgacc tcgccgcgca gaatctcctc caccagccag
     3001 cggagcggca gcacccgcac acccctcacg aacacccggc cctccacgaa cgcctccgtg
     3061 aagcatagta atggagaaac ctccgcgtcc aggatgtcct tcagccggaa gcagccgctc
     3121 caggtctgcg agacgaaatc tttccctggc ggacggccgt tcaacagcaa ctggttgccg
     3181 cgcgcgcgaa cgccgcccga ttggctcttc gtctcgatgc cgaagaaccc gcgaggaccg
     3241 agcgcgacgt ggtctacgtt accaacatcc gggagctgca catcatgaaa gatgtaaaac
     3301 ccgtgctccc gtaacctctc cagctccgcg ccgacctgct tttcggctct tccgcctttg
     3361 agccaggttc gctgggcatc cagggccttg tctcgcgcgt tcttgtgcgc tgccttccag
     3421 ccgaccgcct gcgtcagcgt ctcggcaatc gtccacagga cgatctgctt ccgcgccttg
     3481 cccatcgcgt gctgtcccgc cgaccgcttc tcaagaacct catcgttgac gaccggccgc
     3541 gcggtacgat gcccggaggc cgccgggcgc tccgcgattc tcgtctcgga agcaggactc
     3601 ggaactcgtt cgggcgcctt ctccggacta acctctggtg ttccggccca ggcaagcacg
     3661 tcctccaggc cctcttccga gagggaagtg aagccgccgg ttagcgcgta gcggtggaag
     3721 cccagggtga gatgtggccc cttctgcggg aacttatagg gcacc
//
LOCUS       DASSMB010000112         5140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1162923, whole genome shotgun sequence.
ACCESSION   DASSMB010000112 DASSMB010000000
VERSION     DASSMB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162923"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1532
                     /locus_tag="VFJ72_06990"
     CDS             <1..1532
                     /locus_tag="VFJ72_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7479246.1"
                     /translation="VGVAPGAEWIAAKIFDDNDQATTAGIHQSFQWILDPDGDPATAD
                     APDVVNSSWAMPSPGCDLEFQPDLQALLAAGILPVFAAGNYGPNTGTAASPANYPEAL
                     AVGASDNAGTVQSFSSRGPSSCGEPQGTPQSTFPEVVAPGLDIKAPDLLGGYAYASGT
                     SLAAPHAAGALALLLDADPSLTAAQQREALTASATDVGAAGPDDASGYGLINARSAYD
                     YVRTNFWSLPPDTTPPVISADPAPPASGSVNGAQSVALSSDGGEKVRYTTDGTVPDYT
                     SKLYDGTRINITRTATIRAYAIDAAGNRADASFSYTVRQPTAITLDATRTRLDFGQGT
                     TLSGTLTSNGAPLARKTVEIEQRPVGASAFVSVPNGAVETGADGGFSLSSVKPRQNTE
                     YRAVFAEQPELQSCAMGRRVDVRVMVSAKLSASTVRPGRGVTISGYVRPSHTGSVEVE
                     IKHGGAVVKRKTASLDGDSHYSLRYTPRPVGKYTVSVSYSGHTDHLGGVSPARHFLVS
                     G"
     gene            1599..2057
                     /locus_tag="VFJ72_06995"
     CDS             1599..2057
                     /locus_tag="VFJ72_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET7479247.1"
                     /translation="MKGVVEEIYIAGEGSAEMVRVEEVQTIEGCGIVGDRYYEGTGYW
                     TGLDYVCEVTLISSEDLDRIELETGIRVKNGEHRRNIVTSGVNLDGLRGKRFRIGGAV
                     LEYDRPRPPCRHVQDLTEPGMTRALRGRGGICARVLRAGRIQTADAIEPE"
     gene            complement(1978..3558)
                     /locus_tag="VFJ72_07000"
     CDS             complement(1978..3558)
                     /locus_tag="VFJ72_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7479248.1"
                     /translation="MVSSVFRHGRYTVVVILIVVLIPALVLGLGRSDRTFRKPDTYAN
                     PVPLRVAGLLGKGSGEYATVSPEEVQGASYLSGSAVLRLPDGRLRYVPPGAKEPVTVD
                     SEDPGARAASEESRAWLERGTVPGRTREERRMAERALLDLRLLTQPNGAALAAPTRGW
                     HYVWPRDASWTAAAFAATGHREESYRILRFLAEAQEDDGGWEARYRADGAPVLDGRPA
                     QLDAVGWFPWAVWYWSRDAGGERSAGARTRALWPAVRAASDTASASLGPDGLPPGGPD
                     YWETATWRPNLGTAAPLRTGLRAAASLADELGHPEDARRYANAAARLDLAIDREFAPH
                     GYPRTTDPGSGADAAITFLAPPFAPFEPEVNRAVERAEERLAVPNGGLLPGENWPQDP
                     TVAWTPETALFALSAAASGDEERAGRHLGWLMDHRTSLGAFPEKVNGEGEPRAAAPLG
                     WTSAAVLLALAAEEEPLPVPPAGSAVEDEGTSPLGSSFGGALIGAGALVAATRARWRP
                     QSGSSRPSKPGRRYLPGP"
     gene            3878..4528
                     /locus_tag="VFJ72_07005"
     CDS             3878..4528
                     /locus_tag="VFJ72_07005"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7479249.1"
                     /translation="MTRKRLSRAERRIDTRERLLASALVVFARKGFEGASLEEIADEA
                     GFSTGAVYSAFGGKQELFFALLDRCLEEEKRDIREIFERNDSLEAVVAAIGRWKPGPL
                     ESAEQWRVLATEFWLHAVRDPRARALLAERQRRQRAAAAGQLEGAYKEQGRRLPVEAG
                     LLADAVIGLQIGMAMRAIVDPEAVPEGSYARVLGVLLGTSDASGERTPGEGEEETA"
     gene            4589..>5140
                     /locus_tag="VFJ72_07010"
     CDS             4589..>5140
                     /locus_tag="VFJ72_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7479250.1"
                     /translation="MQQIAPEPVDSVEVTILADNVFDGLLVSEGPARRPSLGPQHLNI
                     STPFMEGGLAFDQPVVQHGFGALVVVRKNGREHRVLFDTGATPDGLAENMRRMDLSPG
                     DVEAIVLSHGHSDHTTGMDGLARALGRANLPVLIHPEFWNRRRLAFPGREPFELPTTS
                     KGALEDAGFEIVEDVRPSFLLDDS"
BASE COUNT          908 a         1768 c         1682 g          782 t
ORIGIN      
        1 ccgtcggggt ggcgccgggc gcggagtgga tagcggccaa gatcttcgac gacaacgacc
       61 aggcgacgac cgccgggatc caccagagct tccagtggat actcgacccg gacggcgacc
      121 ccgcgaccgc cgacgccccg gacgtcgtca acagctcctg ggccatgccc agccccggct
      181 gcgaccttga gttccagccg gaccttcagg cgctgctggc cgcgggcatc ctacccgtct
      241 tcgccgccgg caactacggc cccaacaccg ggaccgccgc cagccccgcc aactaccccg
      301 aagccctggc cgtcggagcc tcggataacg cggggaccgt ccagtcgttc agcagccgcg
      361 gcccgtcctc ctgcggcgag ccccagggca caccccagag caccttcccc gaggtcgtcg
      421 cccccggcct cgacataaag gccccggacc tcctgggcgg ctacgcatac gccagcggga
      481 cttccctggc cgcgccgcac gccgcgggcg cgctcgccct gctcctggac gcggacccgt
      541 ccctgaccgc cgcccagcag cgcgaggccc tcacggcctc cgccacggac gtgggagccg
      601 ccgggccgga cgacgcctcc ggttacggcc tcataaacgc acggtccgcc tacgattacg
      661 tgcggacgaa cttctggtcg ctccccccgg acactacgcc gccggttatc tccgccgacc
      721 cggccccgcc cgcgagcggc tcggtcaacg gcgcccagtc cgtggcgctc tcctctgacg
      781 gcggcgagaa ggtccgctac acgaccgacg gtaccgtccc cgactacacc agcaagctct
      841 acgacggcac gcgcataaac atcacccgga ccgccacgat ccgggcctac gccatagacg
      901 ccgccggcaa ccgcgccgac gcctcgttct cctacaccgt gcgccagcca accgccataa
      961 cgctcgacgc cacacgcacc cgcctggact tcggacaggg cacgaccctc tccgggaccc
     1021 tgacctcgaa cggcgctccg ctcgcccgga agaccgtcga gatcgagcag cgccccgtcg
     1081 gagcatccgc cttcgtctcc gtgccgaacg gcgcggtcga gaccggcgcg gacggcggtt
     1141 tctccctatc cagtgtgaag ccccgccaaa atacagagta ccgggcggtc ttcgccgagc
     1201 agccggagct tcagtcctgt gcgatgggca ggcgagtgga cgtgagggtc atggtctcgg
     1261 cgaagctctc cgcgagcacc gtgcggccgg ggcgcggggt tacgatctcc gggtacgtca
     1321 gaccatcgca cacgggttcg gtcgaagttg agatcaaaca cggcggcgcc gtggtcaagc
     1381 ggaagacggc atcacttgac ggcgactctc attacagcct acgctacaca ccgcgccctg
     1441 tcggcaagta cacggtgagc gtcagctact cgggacacac cgatcacctg ggcggcgtca
     1501 gccccgcgag acacttcctc gtatccggat agactgccgg ctgttaacct cttcaacatc
     1561 gaagagccgg tcgtaaagac gtgggaggga aacgaaggat gaagggcgtg gtcgaggaga
     1621 tctacatcgc cggagaaggc agcgcggaaa tggtgagggt cgaggaggtc cagactatcg
     1681 aaggatgcgg catagtgggc gaccgctact acgaaggcac gggctactgg acgggactcg
     1741 actacgtctg cgaggtcacc ctgatctcct ccgaagacct cgaccgcatc gagctggaga
     1801 caggcatccg cgtcaagaac ggcgagcacc ggcgcaatat cgtcacgagc ggcgtcaacc
     1861 tggatgggtt gcgcggcaaa cggttccgaa tcggaggggc agtcctcgaa tacgaccgtc
     1921 cccgtccgcc gtgccgccac gtccaggacc ttacagaacc cggcatgacg cgggctctca
     1981 ggggccgggg aggtatctgc gcccgggttt tgagggccgg gaggatccag actgcggacg
     2041 ccatcgagcc cgagtagcag ccacgagcgc ccccgctccg atcaaagcgc cgccgaaaga
     2101 agatcccaga ggcgatgtcc cctcatcctc cacagcgctt ccggctggag gcaccggtag
     2161 aggctcctct tcggcggcca gggcgagcaa tacagccgcg ctcgtccagc cgagcggcgc
     2221 tgctgccctg ggttcgccct cgccgttcac cttctccggg aacgctccga ggctcgtgcg
     2281 gtggtccatg agccacccca gatggcgtcc ggccctctcc tcatcgcccg acgccgccgc
     2341 ggagagcgcg aatagggcgg tctcgggcgt ccaggcgacg gtcgggtcct ggggccagtt
     2401 ctcgccaggg agcaagccgc cgttcgggac ggccagccgc tcttccgccc tctcaacagc
     2461 ccggtttacc tccggctcaa acggggcgaa gggtggagcc agaaaagtga ttgccgcgtc
     2521 ggcgccgctg ccgggatcgg tggtgcgcgg ataaccgtgc ggcgcgaact cgcggtctat
     2581 cgccaggtcg agacgggccg ccgcgttggc ataccgccgg gcgtcctccg ggtggcccaa
     2641 ctcgtccgcc aggctagctg cggcgcgcaa gccggtcctc aggggcgcgg cggtcccaag
     2701 gttcggcctc caggtcgcgg tctcccagta gtccgggccg ccgggcggca ggccatccgg
     2761 ccccagcgag gcggaagcgg tatcagaggc ggcccggacc gcgggccaga gtgcgcgggt
     2821 tcgcgctcca gctgaccgtt ctccgccggc atccctggac cagtaccata cggcccaggg
     2881 gaaccatccc acggcgtcga gttgcgccgg ccggccgtcg agcacgggcg ctccgtcggc
     2941 gcggtagcgg gcctcccagc cgccatcgtc ctcctgcgct tccgcgagaa agcgcagaat
     3001 ccggtacgac tcctctcgat gtccggtcgc cgcgaaggct gccgccgtcc agctcgcgtc
     3061 gcgcggccag acatagtgcc agccgcgagt cggggcggcg agcgcggccc cgttcggctg
     3121 tgtgagcagg cgaagatcca gcagcgcccg ctccgccatc cggcgttcct cccgcgtgcg
     3181 accggggacc gtaccccgtt cgagccaggc ccgcgactcc tcgctggcgg cccgcgcgcc
     3241 gggatcttcg gagtccacgg ttacgggctc ttttgcgccg ggcggcacgt aacgcaagcg
     3301 gccgtccggg agcctcagca ccgcgctgcc ggacagatag gaagcgccct ggacttcttc
     3361 cggtgagacc gtcgcgtatt cccccgatcc cttcccgagc agcccggcta cccgcagcgg
     3421 aaccgggttg gcgtaggtat cgggtttgcg gaaggtgcgg tccgatcttc cgagccccag
     3481 gacgagcgcc ggaatgagaa ccacgatcaa gatgactacg actgtatagc gcccgtgtcg
     3541 gaacacactg gagaccatac tagatcctcc agcagatgct atgaccggac ccaacttcgt
     3601 ccggcgctcc tcatatttgt catccacatg gcagaaaacg tcttcattgt tgtcaccagt
     3661 aagaaatacg ctagccgtcg aaaccaaaaa tgtggctgac ctgcattacg caaacctgca
     3721 tccgtactcg ctcaatcgtc aagcaacgat tttagatcgt gtttttacca acgtgagaac
     3781 ggcaccgaag atcctctcac gagtatccgt tgatttatgt aatttcatta ttataataat
     3841 gttgttattt gaactcagta ccgagaagtc gggtgacatg acgaggaaga gactaagccg
     3901 ggcggagaga aggatcgata ccagggagcg cctgctggcg agcgccctgg tggtgttcgc
     3961 ccgaaaggga tttgaggggg cttccttgga agagatcgcc gacgaggcgg gtttctccac
     4021 gggggcggtc tactctgcgt ttgggggtaa gcaagagttg ttcttcgctc tgctcgaccg
     4081 gtgtctggag gaagagaagc gcgacatacg tgagatattc gagcggaacg acagcctgga
     4141 ggccgtggtc gcggcgatcg gccggtggaa gccgggtccg ctcgagagcg ccgagcagtg
     4201 gcgcgtgttg gccactgagt tctggcttca cgccgtccgg gatccccggg cgcgggcgct
     4261 gctggccgag cggcagcgca ggcagcgcgc cgccgcggcc gggcagttgg aaggggccta
     4321 caaggagcag ggtcggcgat tgccggtcga ggccgggctg ctggccgacg cggtcatagg
     4381 gttgcagatc gggatggcca tgcgggcgat cgtcgacccc gaggccgtcc ccgaaggctc
     4441 ctacgcgagg gtacttggcg tcctgctggg gacgtcggac gcctcgggag agcgtacacc
     4501 gggggaggga gaggaggaga cagcgtaaga gcatcgtacc gttgcacgaa accggttagc
     4561 gagaacccct cgaaaaggtg gtgaaaccat gcagcaaata gcccccgaac ccgtcgattc
     4621 ggtcgaggtc acgatcctgg ccgacaacgt cttcgacggc ctcctcgtca gcgagggacc
     4681 cgccagacgt ccctccctgg gcccgcagca cttaaacata tccaccccat tcatggaggg
     4741 cgggctcgcc ttcgaccagc cggtcgtcca gcacggtttc ggggcgctcg tcgtcgttag
     4801 gaagaacggg cgcgagcacc gggtgctctt cgacaccggg gctacgcccg acggcctcgc
     4861 ggagaacatg cgccggatgg acctctcgcc cggcgacgtc gaggcgatag tcttgagcca
     4921 cggccactcc gaccacacca ccgggatgga tgggctcgcg cgggctctcg ggcgggccaa
     4981 cctccccgtg ctcatccacc ccgagttctg gaacaggagg cgactcgcct tcccgggccg
     5041 cgaaccgttc gagctaccga ccacgagcaa gggggcgctg gaggacgctg gcttcgagat
     5101 cgtcgaagat gtgcggcctt cgttcctgct cgacgactcg
//
LOCUS       DASSMB010000113         7987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1545623, whole genome shotgun sequence.
ACCESSION   DASSMB010000113 DASSMB010000000
VERSION     DASSMB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7987
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545623"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFJ72_07015"
     CDS             complement(<1..189)
                     /locus_tag="VFJ72_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7479251.1"
                     /translation="MFGIGATEMVIVGILFLVIFGPSKLPDMARDIGRFVSEARRSVD
                     EFKADLVSEEASGEEDGRG"
     gene            complement(194..445)
                     /locus_tag="VFJ72_07020"
     CDS             complement(194..445)
                     /locus_tag="VFJ72_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7479252.1"
                     /translation="MFAGLASPPHLIIALAIILLLFGAKRIPGLAKGFGTGVKELREG
                     LSTGGDARAEAPGSEKVRVQARAGSGDETVHGHAGQRGL"
     gene            1376..1561
                     /locus_tag="VFJ72_07025"
     CDS             1376..1561
                     /locus_tag="VFJ72_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479253.1"
                     /translation="MRGLLCRCEHRLDATDDTALLGLVQRHFRWEHPTIPIEDETLED
                     IAATHTYGLEDVEAPAG"
     gene            complement(1640..2185)
                     /locus_tag="VFJ72_07030"
     CDS             complement(1640..2185)
                     /locus_tag="VFJ72_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HET7479254.1"
                     /translation="MSSNVESEQSYSEEAVPYRGEQPYGMPDDLVVPGILDLDSDERL
                     WVPQATDVSFRPLLLSVSEGYFVNLLRVRKSAILSRHRHAGPVHALTLKGSWHYLEHE
                     WWAEEGGYSFEPPGETHTLEVPDGVEEMVTLFHVTGAYVYVDPDGNVVGIEDVFTKLE
                     AAREHYEEVGLGAGYVERFIR"
     gene            2478..2708
                     /locus_tag="VFJ72_07035"
     CDS             2478..2708
                     /locus_tag="VFJ72_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479255.1"
                     /translation="MRITEVKVSGRLALACGGCGELILFLGRESDWFETGEDGRPRIF
                     PCGGCGRDLTLASRVVASGTGSASGSPPGRPG"
     gene            complement(2947..3954)
                     /locus_tag="VFJ72_07040"
     CDS             complement(2947..3954)
                     /locus_tag="VFJ72_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7479256.1"
                     /translation="MSTALEGPATGLLPAPPSEAVGPPNAATVRPAPMPNFSGAETDE
                     RLVELWLARKAPSTRRKYAEDLAKFRDFTEGKPLSAVTLSDLHDFASFISVLMAPSSQ
                     GRVLATTKSLLSFANKVGYLPFNVGAAVELPKVKDTLSERILTEAEAHRIMNAPKSAR
                     DRALISLLYAGALRREEAVHLKWRDLKDRDDLGAGVGQATIFGKGSRTGVTLLPASVY
                     SQVLALRKVTERTNGARTERHAGEDEPVFRSRKKKNGLGGHLEVSQVNRIVARAAEDA
                     GIERAVSPHWLRHAHASHAHARKTDLALIRDTLRHSSIATTGRYLHARPNDSSALHLG
                     L"
     gene            complement(3951..4571)
                     /locus_tag="VFJ72_07045"
     CDS             complement(3951..4571)
                     /locus_tag="VFJ72_07045"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET7479257.1"
                     /translation="MSAGKFDGMLREGCGGQMLPGNLEINRHRFTARQYRAMAEAGVL
                     GEDDRVELVDGEIVDMAPIGSRHLACVVALNHLLVGASGGRFFVSVQNPVRLGERDEP
                     QPDLSLLGRRPRPEASAPPGPEDVLAVVEVSDTTLSYDRNTKLPLYARAGIPEAWIVD
                     LAGQKVEVYYHPDPDGYRTARSFEAGEWIISGAVEGLSVAVDEVLG"
     gene            complement(4699..5211)
                     /locus_tag="VFJ72_07050"
     CDS             complement(4699..5211)
                     /locus_tag="VFJ72_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7479258.1"
                     /translation="MRAGPRPARHPPGQEFLASRGARFLSYLLDTNVISEARKGARAD
                     AGVLRWLSGVGQQELYISVLVVGEIRQGVERLKRRDPDQAAVFEAWLAELRGSYAERI
                     VPITVEDADEWGRMTAPDPLPAVDSLMAAQAKSRGMTLVTNNTRDFERTGVALLDPFD
                     ARLPFHTIIV"
     gene            complement(5136..5375)
                     /locus_tag="VFJ72_07055"
     CDS             complement(5136..5375)
                     /locus_tag="VFJ72_07055"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET7479259.1"
                     /translation="MQEAKQRFSELIRRALEEGPQTVTKHGEEVVVVVPAKDYERLVA
                     ESGKDFKRFLMCAPDLDRLDIRRDKSSSRVVELGS"
     gene            complement(5586..5753)
                     /locus_tag="VFJ72_07060"
     CDS             complement(5586..5753)
                     /locus_tag="VFJ72_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479260.1"
                     /translation="MTEAQAAIEMRILLLSDFPHEMVIGGAAGGLPVLAAAGVKTLPD
                     HRERLKGIRDN"
     gene            complement(5786..6181)
                     /locus_tag="VFJ72_07065"
     CDS             complement(5786..6181)
                     /locus_tag="VFJ72_07065"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7479261.1"
                     /translation="MDRLFLDANVLFSAAYREDAGVRRLWGLPETELVTSAYAVEEAR
                     RNLGSAEQRADLDKLLMAVRVTNLLADPAGRPEIEGSGLPEKDLPILRAAVAVSATYL
                     ISGDRQHFGHLFGRTVAGVLVARPADYLA"
     gene            complement(6183..6503)
                     /locus_tag="VFJ72_07070"
     CDS             complement(6183..6503)
                     /locus_tag="VFJ72_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET7479262.1"
                     /translation="MGENGAEAIVDTSKVGKRGAVVIPAPLRRRFGIEEGTLVVAEAT
                     EEGVLIRPAVAVPIEIYSAERKARLLLENATDAEDYARAARVVREEMGLDPDLLGIEL
                     PNQR"
     gene            complement(6654..7316)
                     /locus_tag="VFJ72_07075"
     CDS             complement(6654..7316)
                     /locus_tag="VFJ72_07075"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7479263.1"
                     /translation="MQSCQQCSSASPSALTFRDGELHATLDGNPIRAGFVLDEFEVRL
                     SPHGVVYELFKLRPPTVDELGSGGATARGKPVSSVPRARRGAVAGSFPSAKNVAALLM
                     RRKERLSAEQAAYLERLGEVDDAVANTRRLTQDFAAMVRSLQGQRLDGWLEEAENSEA
                     SVMRRFAAGLRKDLAAVRAGLTEPWSNGPVEGFVHKLKLLKRQGYGRAGFDLLRARVL
                     AA"
     gene            complement(7320..>7987)
                     /locus_tag="VFJ72_07080"
     CDS             complement(7320..>7987)
                     /locus_tag="VFJ72_07080"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HET7479264.1"
                     /translation="GTSAATDHHALIEGDHGPWLLSLAIFTSFWLVMVAAMMLPANMN
                     ILAALGSVAPGARSRALATGTFLAGYALVWASFGAGAFAADTAVHALAYQWHWLHEHE
                     WIILATTLATVGLYQLIPTKRRYLERCRHQTDYISRHPDDTPSATLLQGLHYGKCELA
                     CCWAPMLLMVAVGHHLVWMLLLAGVMLAEKCFTRGVLLARLVGAALVLCSPLVFLVDY
                     CCH"
BASE COUNT         1418 a         2659 c         2440 g         1470 t
ORIGIN      
        1 tccccggccg tcctcctcac cggaggcctc ttcggagacg agatccgcct tgaactcgtc
       61 caccgagcgg cgggcttcgc tcacgaaccg cccgatatcc cgggccatgt cagggagctt
      121 gctgggacca aagatgacca aaaagaggat ccccacgatc accatctccg tagccccaat
      181 accgaacacg acgctagaga cctctctggc cggcgtgccc gtggaccgtc tcgtcccccg
      241 acccggcacg ggcctggaca cgaaccttct cacttccggg cgcctcggcc ctggcgtcgc
      301 cgccggtcga gagtccttcg cgcaactcct tgacgcccgt tccaaagccc ttcgccagcc
      361 ccggtatccg cttggctcca aagagcagca ggatgatcgc gagcgcgatg atcagatgag
      421 gaggcgatgc gagtcctgcg aacattggcg ttcatccttt gcttcgctta cgtgacgccg
      481 gttgtttgta tgtttgtatg acaccggttc tcaggattac gatccctggg gatggatcgg
      541 atcagcatct tctgggagaa ttgtcggcag ggtgagcgcc ttgccaggct actgggtgat
      601 tagcggccga cgcggaaagg gcttgcctca cgcggtaatg ttgcccccgc cgtacttttc
      661 cgggattgcg caccataggc gcacgctatc catatccgcc aaccgattcc cccgttattg
      721 tggtccaaca gcttcaggtc ttcgggcgtg tgttaccgtt tccccgtcca acaactcaga
      781 gcggaccgat caaatcacct tgcccagacg aaggtatgga gccggctcgc gaaagccgac
      841 gacgattgcg ggagggagat actaccggtg tcgtagactt cacgatgcac cctagcttgc
      901 tggcgctccg cgcgcagcga ctgccgacga catcgagcgt catcctcagc tcgtcaacgc
      961 cccggttgtg gatcccgcag tccaacttgc gcccgcgcgg accttggaca tcccgcgaaa
     1021 acgtttaatg atggcgggct gctcgccgag atggctcaga aaggcattcc ggcaccgttg
     1081 tgggcgacct attaataagg taaaggtata cccacacgtg tgatgggcca gcgcagcctc
     1141 tccaagggca tactgtggac ctctggtctt cagcgtttca tcacgggtta tataatgcgg
     1201 agctatcgat gagcccgctc taggcgtcga atgctccctg caacacggcg cccggacgtc
     1261 ttggggcaaa ttgcccattg gctctcgaac ggaaagcatc taacctgcct gttgccggac
     1321 gagcgctcat gggtgacgcc gctcggctga gaattggatc gggaaggggg tatggatgcg
     1381 cgggttgctc tgccgttgcg aacaccgcct ggatgctaca gacgatacgg ccctgctcgg
     1441 gctggtccag agacacttcc gctgggagca tcccaccatc ccgatagaag acgagaccct
     1501 tgaggatatt gccgccaccc acacctacgg gctggaggac gtggaggcgc ccgccggctg
     1561 agatctcgat ctctcctccc gacccccttc tggaagggct cgcaacgtgg gctgtggcag
     1621 tcctgtggca gtcagcaggt cagcggatga agcgctcgac gtagccggcg ccgagcccga
     1681 cctcctcgta gtgctcgcgc gcggcttcga gcttggtgaa cacgtcctcg ataccgacca
     1741 cgttgccgtc ggggtcgacg tagacgtagg cgccggtgac atggaacagt gtgaccatct
     1801 cctccacccc gtccggaacc tccagggtgt gcgtctcgcc gggcggctcg aacgagtagc
     1861 ctccctcctc ggcccaccac tcgtgctcca gataatgcca gctgcccttg agcgtcaagg
     1921 cgtgcaccgg tcccgcgtgc cggtggcgcg acaggatggc gctcttgcgc acccgcagga
     1981 ggttgacgaa gtacccctcg ctcaccgaca gcagcagcgg ccggaagctg acgtccgtag
     2041 cctgggggac ccacaggcgc tcgtcggagt cgaggtctag aattccagga accaccaggt
     2101 catccggcat gccgtatggc tgctcgccac ggtacgggac cgcttcctca gagtaggact
     2161 gctcggactc gacattcgaa ctcattcgct cctcctccaa cgttccccgg tgttctgcat
     2221 ccgcaatccc ttgattccca actatcagaa accgtaacca ccggcgccgc ccgcccttgg
     2281 ctgccttgga agcgatggcg aacaacgccc tagatcccct gcgcaagcca caggccataa
     2341 ccctccatct catccttacc tgaccctcaa ccgaggttta cctcagcccg atacaccttg
     2401 cgaggaaggt tagatcgcgg gtaggctcaa agaagcagga caggatcaca tagggagatg
     2461 gcgatagggg gacggcgttg cgtatcaccg aggtgaaggt atccggacgg ctggccctgg
     2521 cgtgcggcgg ctgcggggag ttgatcctct tcctgggccg cgagagcgac tggttcgaaa
     2581 ccggcgagga cggccgccca aggatcttcc cgtgcggcgg gtgcggaagg gacctcaccc
     2641 tcgcgagccg ggtcgtcgcg agcggaacag ggagcgcgtc cggtagcccg ccggggagac
     2701 ccggatagtc ctatggggtc acctgtatcg gcactaccgg tagggcttgg acatcagcgc
     2761 agccgttact gatgatcctg gcgatgcggg acattgcgcg gggtgtaagc ggctttaaga
     2821 caggtatgct agatttgccc gcgcgaggtt ggctcctgag cgcctcggct gcctctccga
     2881 aggatggggg atactccagt catcgatggc cgaacgccgg gtcgggagca gtcgagggag
     2941 ggcgcttcag aggcccaggt gcagcgccga ggagtcgttg ggcctcgcgt gcaggtagcg
     3001 tcccgtggtc gctatcgagg aatggcgcaa ggtgtcccgt atcagggcca gatccgtctt
     3061 cctggcgtga gcgtgggagg cgtgggcgtg cctgagccag tgggggctca cggccctctc
     3121 tatcccggcg tcctcggccg ccctggcgac tatccggttg acctggctga cctccaggtg
     3181 accgccgagg ccgttctttt tctttctgct gcggaacacg ggctcgtcct cgccggcgtg
     3241 ccgctcggtc cgggccccgt tcgtcctctc cgtcaccttc cggagcgcga ggacctggga
     3301 gtagacgctc gccggcagca gggtgacgcc ggtcctggag cccttgccga agatggtcgc
     3361 ctgcccgaca cccgccccga ggtcgtcgcg gtccttgagg tcgcgccact tcaggtgcac
     3421 cgcctcctcc cgcctgagcg cgccggcgta gagcagcgag atcagggccc ggtccctcgc
     3481 ggacttcggc gcgttcatga tcctgtgggc ctcggcctcg gtcagtatcc tctcggacag
     3541 ggtgtccttg accttcggca gctccacggc ggccccgacg ttgaagggca ggtagcccac
     3601 cttgttggcg aaggagagca ggctctttgt ggtggcgagg acccgcccct ggctcgaggg
     3661 ggccatcaag accgagataa aggaggcgaa gtcgtgcagg tccgagagcg ttacggccga
     3721 cagtggcttc ccttcggtga agtcccgaaa cttggcgagg tcctccgcgt acttgcgccg
     3781 ggtggagggc gccttgcggg cgagccagag ctcgacgagc cgctcgtcgg tctcggcgcc
     3841 ggagaagttg ggcatcggcg cggggcggac cgtggccgcg ttcggtgggc caaccgcttc
     3901 actcggaggc gccggcagca gccccgtcgc cggtccctcc agcgcggtac tcacccgagc
     3961 acctcgtcga ccgcaaccga gaggccctct acggctcccg agatgatcca ctcgccggct
     4021 tcgaaagacc tcgccgtgcg gtagccgtcc ggatctggat gataatagac ctcgaccttt
     4081 tgccccgcga ggtccacgat ccacgcctcc ggaatgcctg cccgggcata tagaggcagc
     4141 ttggtgttcc tgtcgtagga gagggttgtg tccgaaacct ccaccacggc gagaacgtcc
     4201 tccggaccag ggggcgccga agcctccggc cgcggcctgc gcccaagcaa gctcaggtcc
     4261 ggctggggct cgtcgcgctc acccagcctc acagggttct gaacgctcac gaaaaacctc
     4321 ccgcccgacg caccaaccag cagatggttc aaagcgacca cgcatgccag gtggcgcgaa
     4381 cctatcggcg ccatgtccac gatctcgccg tcgacgagct ccacccgatc gtcctcaccg
     4441 agtaccccag cctcggccat cgcgcggtac tgtcgtgccg tgaacctgtg cctgttgatc
     4501 tcaaggttcc ctggcagcat ctgtcctcca cacccctccc tcagcatgcc atcaaactta
     4561 cctgcggaca cgatacacaa cctatagtat gaacgcaagg atagtggtat acccttgcgt
     4621 tcaaaatgcc ctcgaaaggc ggctctccgg cgccctgccg ccccgcgaac gggcgcggat
     4681 cgaaccacgc ccggtggctc acactattat tgtgtggaaa ggtaaacggg cgtcgaacgg
     4741 gtcgagcaag gcgacgccgg tgcgctcgaa atcgcgggtg ttgttggtga ctagcgtcat
     4801 gcccctggac tttgcctgtg cggccatcag gctgtcgacg gccggcagcg gatcgggcgc
     4861 ggtcatccgg ccccattcgt cggcgtcctc gacggtaatc gggacgatgc gctcggcgta
     4921 ggatcctcgc agctcggcga gccacgcttc gaagacggcc gcctggtcgg ggtcgcgcct
     4981 tttgaggcgt tcgacgccct gacggatctc gccgacgacg agcacgctga tgtacagttc
     5041 ttgctgcccg acgcctgaga gccagcggag cacgccagcg tcggcgcgcg cgcccttgcg
     5101 cgcctcggag atgacgttgg tgtccagcag gtagctcagg aaccgagctc cacgactcgc
     5161 gaggaactct tgtcccggcg gatgtcgagc cggtcgaggt ccggcgcgca catcaagaac
     5221 cgcttgaagt ccttgccgct ctcggcgacg agccgctcgt aatccttggc cgggaccaca
     5281 accacgacct cctcgccatg ctttgttacg gtctgcggcc cttcctccag cgcgcgccgt
     5341 atcaattcgc tgaacctctg cttcgcttcc tgcacctgcc aagtcatccc gccaactcct
     5401 actctgtcca ctctgtccag aattatatcg cattggtaga ccgtaacgcc gcggccttct
     5461 cggtttccgg gggcgagaaa caactccccg aaggcgcgat tcgatttcac cactcatgct
     5521 cggtccgttc ggggatctcg gccgacgcgt gtttcgagcc tctggccggt ccgatagaag
     5581 tagagctagt tgtcccggat ccccttgagc ctctccctgt gatccggcaa ggtcttgacg
     5641 cccgccgccg ccaggaccgg caagcctcct gccgcgcccc cgataaccat ttcgtgcggg
     5701 aagtcggata agagtagtat tctcatctcg attgctgcct gtgcttccgt caccgcggct
     5761 tcttgcggga gcgtcacccg gctctttacg ccaaataatc ggccggacgg gcgacgagca
     5821 cgccggcgac cgttctacca aagagatggc caaagtgttg gcggtcgccg gagatcaggt
     5881 acgtggcgct cacggcgacc gcggcgcgca gtatcggcag atccttctcc ggtaatcccg
     5941 aaccctcgat ctcggggcgc ccggcgggat ccgcgagcag gttggtgacg cggacggcca
     6001 tgaggagttt atcgaggtcc gcgcgctgct cggccgaacc gaggtttcta cgcgcttcct
     6061 cgacggcgta ggccgacgtc acgagctccg tctccggaag tccccagagc ctccgcaccc
     6121 cggcgtcttc gcggtaggcc gccgagaaga gcacgttggc gtccaaaaag agccggtcca
     6181 cctcagcgtt ggttcggtag ctctataccg agcaggtcgg gatccaagcc catctcctcg
     6241 cgcaccaccc gagcggccct cgcgtagtcc tcggcgtcgg tggcgttctc caacaacagg
     6301 cgcgccttgc gctcggcgga gtagatctct ataggcaccg ccaccgccgg acggatcagg
     6361 acaccctcct ccgtggcctc cgccaccacc agcgtcccct cctctatccc gaagcgcctc
     6421 ctcaagggcg ccggtataac caccgcgccc ctcttcccga ctttactagt gtccacgata
     6481 gcctccgcgc cattttcgcc caattatctg attatctggc atcaaggcta ttttagcacg
     6541 ggcagggcat ctatctgggc ggctacctca cgaaacctcg cctttaagtg tgggctctgt
     6601 cgggttcttg gtgaagcggc gcttcctatg ctgtgacgac gctcgagcag cgatcaggcc
     6661 gccagaaccc tcgccctgag taggtcgaaa cccgcccgtc cgtacccttg tcgcttcaac
     6721 agtttcagct tgtggacgaa tccttccacc ggcccgttgc tccaaggttc tgtgagcccc
     6781 gcccgaactg ccgccagatc cttcctgagt cccgcagcga aacgccgcat caccgaggcc
     6841 tccgagtttt cggcttcttc gagccagccg tccaggcgtt ggccttggag gctgcggacc
     6901 atcgccgcga agtcctgcgt cagccttcgg gtgttcgcta cagcgtcgtc gacctccccg
     6961 agccgttcga ggtacgctgc ctgctccgct gagagcctct ccttgcggcg catcagcagc
     7021 gcagcgacgt tcttcgcact cgggaacgag cccgccacgg cgcccctcct ggccctcggc
     7081 accgacgaca ccggcttgcc ccgggcggtc gccccgcccg atccgagttc gtccacggtg
     7141 ggcggacgga gtttaaagag ctcgtagact accccgtggg gcgagaggcg cacctcgaac
     7201 tcatccaaaa cgaatccagc cctgatgggg tttccgtcca gcgtggcgtg cagctcaccg
     7261 tcgcggaagg tcagagccga aggggaagca gatgaacatt gctggcagct ctgcattctt
     7321 cagtgacaac aatagtccac gagaaatacg agtgggctgc acagcaccag cgctgccccg
     7381 acaagccttg caagaagcac gcctcgagta aagcacttct ctgcgagcat caccccggcg
     7441 agcaacaaca tccagaccag atggtgtcca accgcaacca tgagcaacat tggcgcccaa
     7501 caacaagcca gctcacactt cccgtaatgc agcccctgca atagggtagc ggacggcgta
     7561 tcgtcgggat gacgggatat gtagtcggtc tgatgtcggc accgctcaag gtagcgacgc
     7621 tttgtgggga tcagctggta gagacccaca gtcgccaggg tcgtcgccaa gataatccac
     7681 tcgtgctcat gaagccagtg ccactgatag gctaaggcgt gaaccgcggt gtccgccgcg
     7741 aaagcgccgg caccgaaaga ggcccagacc aaagcgtaac ctgcgaggaa tgttccggtc
     7801 gccagcgcgc ggctcctcgc accgggcgca acgcttccga gggcggcgag gatgttcatg
     7861 ttcgcgggca acatcatggc cgcgaccatc accagccaga aagaggtgaa tatggcgaga
     7921 gacagtagcc agggcccgtg atctccttcg attaaggcgt ggtggtcggt ggcggccgag
     7981 gttccca
//
LOCUS       DASSMB010000114         9856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1672352, whole genome shotgun sequence.
ACCESSION   DASSMB010000114 DASSMB010000000
VERSION     DASSMB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9856
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1672352"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VFJ72_07085"
     CDS             complement(<1..1074)
                     /locus_tag="VFJ72_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HET7479265.1"
                     /translation="MVGASLPRVVVAGTHSGVGKTTVASGLMAALSEKGYGIAPFKVG
                     PDFIDPSYHTLAAGRPGRNLDAFLSGPELIGPLFAHGAQGADVAVIEGVMGLYDGKSG
                     GGELASTAHVAKLLDAPVVLVVDARAMARSAAAMVHGYATFDPDVRVSGVILNRVGSA
                     THEKMLRDALEPLGIPVLGVLRRDAAIHTPDRHLGLVPVAERREEARRSLDALGAVIS
                     RSCDLEAILRLARSAGPLEADAWSPESPEEHRTEGVRIGVATGPSFSFLYRENLELLE
                     GAGAEVITFDPTADEDLPDGTDALYLGGGFPETYAEALSANEPMREIVRRFASEGRPI
                     VAECGGLLYLARELDGHPMCGVLD"
     gene            complement(1068..1649)
                     /gene="cobO"
                     /locus_tag="VFJ72_07090"
     CDS             complement(1068..1649)
                     /gene="cobO"
                     /locus_tag="VFJ72_07090"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET7479266.1"
                     /translation="MSEVRESREPRERRLRKRSNRERPLIIVNTGHGKGKSTASFGIM
                     LRGWARGYRIGVYQFVKSGKWKVGEQKAAETLGNIDWFKMGDGWTWTSKDLVESADKA
                     REGWEEVKRRLADETYDMLLLDEFTYPMKFGWVDTGEVIEVLKNRPGFQHVIITGRDA
                     PPELVEISDLVSEVNKVKHPMDEGYRGQKGIEW"
     gene            complement(1646..3592)
                     /locus_tag="VFJ72_07095"
     CDS             complement(1646..3592)
                     /locus_tag="VFJ72_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative cobaltochelatase"
                     /protein_id="HET7479267.1"
                     /translation="MSAYSHPYPFSAIVGQEDLKTALLINAVSPEVGGVLVRGEKGTA
                     KSTAVRALAKLLPQIRVVAGCLYSCDPEAPNPECPAGPHPPDAPVEERPVRLVELPVG
                     ASTDRLAGTLDIEKALSEGRKAFEPGLLAAAHRGILYVDEVNLLSDHLVDLLLDVAAM
                     GVNHVEREGVSVRHPSRFILVGTMNPEEGELRPQLLDRFGITVEVSGSPEPSERVEVV
                     RRRLHYEAGPEEFAAKWSAADTELAATVVAARGRLPGVHLSEDTLYRISALCAELGVD
                     GLRGDIVTAKTARALTAWDARDEVVLEDVKRAALLALSHRRRRGPFEQPGIEPEELEN
                     ALSDPEDPEPPDDPGGGDTPPPESDGQNGEVSFTEPSESRIGSGEKAHGASEMFDPVR
                     LEAPEKGRGGPLGRRSRISGAFGHPVGDRERGANSKDVALAATVRAAAPHQKARGRRG
                     PGLAVRPYDLRENVREGREGNLILFLVDASGSMAAKKRMSAVKGAVLSLLNDAYQRRD
                     KVGLISFRGTGAQILLPPTSSVNLAASRLEELPTGGRTPLAAGLEKAAEVLKRERLRD
                     RERRPLLVLLTDGRATAGPDPRVAASGLRSLGATAFVVDTEEGYVRLGMAGEVADALG
                     ARCLRLEELRAESLVELVERRRVA"
     gene            complement(3589..7332)
                     /gene="cobN"
                     /locus_tag="VFJ72_07100"
     CDS             complement(3589..7332)
                     /gene="cobN"
                     /locus_tag="VFJ72_07100"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET7479268.1"
                     /translation="MADRLLFLTTADTEILAAAKAAERLPEGFPKVRCANPVSVEDPV
                     DLFESALPEARAVMVRLLGGRRAWPEGLEELRRRCSELHIPLLAFGGEAEPDAELTAV
                     STVPSGTVLEAFEYLRHGGVRNMENLLRFVADTVLMEGYGFEPAAPLPEMGVYHPRLP
                     EGSPLEDLISLHDPERPTVGIVFYRAHWMSGNTAFVDTLVEALAEAEANALPVYCYSL
                     RADADGGVPALEMLKGRVDCLVTTVLAGGGSNAADARKAGSPEDWLEWEVPALEDLGV
                     PVVQGICATSSHKTWTHADAGLSPLDTAWQVAIPEFDGRIVGVPFSFKERVGADSSVG
                     APLTLYQADPERTARLAGLATRFARLSRVPNAEKRIAVLLSNYPTKHSRVGNAVGLDT
                     PASAIKLLGAMRDAGYIVNGVPEEGDALIHSLIAAGGHDLEFLTADQLEGAIGRLDAR
                     RYAEWFARLPEDLRSGVEEHWGAPPGELYVDGDEFVVAGLRFGNVFIGIQPPRGFGEN
                     PIAIYHDPDLPPTHHYLAAYWWLIEEFCADAVVHLGKHGTLEWLPGKSLGLSPSCAPD
                     AALKDVPLFYPFVVNDPGEGTQAKRRAHATVIDHLIPPMTRADTYDDLAKLEQLLDEY
                     YQVETLDPSKLPAIRVRIWEVLRDAELHRDLGVEEQPEEFGDFLTHVDGYLCEIKDLP
                     IRGGLHILGEPPAGEPLRHLVASILRIGSGQMSGLRRAVGDSYGLDERALSENGGARV
                     TVPAALAERFPGVVATASDLLDRLEEAVQSLLLAMEERGWDAEAAGTVCEEVLGVADA
                     GVERSLRFAADEVVPRLMRTPEEMGNLLSGLAGGYVPAGPSGSPTRGLVNVLPTGRNF
                     YSVDPKALPSALSWEVGQGLAENLLQRYLEEEGRHPETVGIVVWGTAAMRTQGDDIAE
                     ILALLGVRPVWNEESRRVTRLEVIPLEELGRPRVDVTVRISGFFRDAFPNLISLMDEA
                     FTTVAALDEPDEMNFVKKHASEEKADGADDRRSTTRIFGSKPGAYGAGLLPLMDARNW
                     RDDADLAEVYAVWGGYAYGKGLDGVEAREAMESNLRRTEVAVKNIDNREHDLFDSDDY
                     FQYHGGMIAAVRALTGRDPKAYIGDSADPSRVKTRTLAEEAKRVFRSRVANPKWISAM
                     QRHGYKGAFELSATVDYLFGYDATANVVEDWMYRDVTRKYILDEQVRDFMQQSNPWAL
                     RAISERLLEAAERGLWSEPDPEDLDALKRAYLENEGMLEESGS"
     gene            complement(7332..9002)
                     /locus_tag="VFJ72_07105"
     CDS             complement(7332..9002)
                     /locus_tag="VFJ72_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HET7479269.1"
                     /translation="MSGAGGALLVAGTHSDAGKSVVVAGICRWLVREGVKVAPFKAQN
                     MALNSYVTREGAEIGRAQAAQAAAAKIEPEAAMNPILLKPSAERETQVVVRGKPWAMA
                     SARSYGAMKRELLPVVLKSLEDLRRRFEVVVCEGAGSPAEINLRENDLANMGLARAAH
                     LPVLVVGDIDRGGLFASLYGTLALLSLEDQALVAGFLVNKFRGDPAVLAPGLARMKEL
                     TGRPFLGTLPFVRGIGIDGEDSLALDVPRPVKPPLGRDVLRVAVARLGRISNFTDLDA
                     LAHEPGVRVRFTESPEELLEADLAVLPGTKATVADLEMLRSRGLDRALSERARLGLPV
                     LGICGGYQMLGERITDGIESGEGETAGLGLLPVETVFEEEKVLARPEGSAPGFGNEEV
                     SGYEIHHGRVHRHGGETLFTGDGFDEGCAVETTFGTSWHGILESDGFRRAFLRWISEQ
                     RDLDWTPGDESFAAAREAQLEKLGDLIAENVDREALLRLIDDGAPRRMPVVSGQLVGS
                     RLSGVDGQRNPDGDTPRLGAPATENGKVSASDKAAFLAAGSGKQRSES"
     gene            complement(8999..9700)
                     /locus_tag="VFJ72_07110"
     CDS             complement(8999..9700)
                     /locus_tag="VFJ72_07110"
                     /inference="COORDINATES: protein motif:HMM:NF021039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HET7479270.1"
                     /translation="MFSAYLRRGMAAGLLAGLLAGLFALFVGEPLLDRAIEMEHASGG
                     HSEEVFSRATQKVGLFFATGLFGVTVGGIFGLVYAVLRDRLSSATDWARSNSLSAAIF
                     AGAVLIPFLKYPANPPTVGDPATIGDRTIAYFTMVALSLLALLVAWISARLLRERGIE
                     TPPRQLAVGVGLAVVVVFLFVTLPDAASPGDFPAGLLWSFRLSSLGTQLVLWTGLGVV
                     FGLLCERANRKSGVA"
BASE COUNT         1572 a         3411 c         3258 g         1615 t
ORIGIN      
        1 gtcgaggacg ccgcacatcg ggtggccatc cagctcgcgg gcgaggtata gcaggccgcc
       61 acactcggcc acgatgggac ggccctcgga ggcgaaccgc cggacgatct cgcgcatggg
      121 ctcgttcgcg gagagggctt ccgcgtaggt ctccgggaag ccgccgccga ggtagagggc
      181 gtcggtgccg tcgggaaggt cctcgtcggc cgtggggtcg aaggtgatga cctccgcgcc
      241 cgcgccttcg agaagctcca ggttctccct gtacaggaag ctgaatgacg gtccggtggc
      301 gacgcctatt cggacaccct ccgtccggtg ctcctccggc gactccgggc tccaggcgtc
      361 cgcctccagc ggtccggcgg agcgggcgag gcgcagaatg gcttcgaggt cgcaggagcg
      421 ggagatcacg gcccccagag cgtcgagcga gcgccgggcc tcctcgcggc gctcggcgac
      481 cgggacgagg ccgaggtggc ggtctggcgt atgaatggct gcgtcgcgtc gcaggactcc
      541 gaggacgggg atgccaagcg gttcgagggc gtcgcggagc atcttttcgt gggtggcgga
      601 gccgacgcgg ttcaggatca ctccggagac ccgcacgtcc gggtcgaagg tcgcgtagcc
      661 gtgaaccatc gccgccgccg aacgggccat cgcccgcgcg tccacgacca gcacgaccgg
      721 cgcgtcgagg agcttggcga cgtgggcggt cgaggcgagt tctccgccgc cgctcttgcc
      781 gtcgtagagg cccatgacgc cctcgatcac cgccacgtcc gcgccctgcg cgccgtgggc
      841 gaagagcgga cctatgagtt ccgggccgga gaggaaggcg tcgaggttgc gtccgggacg
      901 tcccgctgcg agggtatggt aggagggatc tatgaagtcc gggccgacct tgaagggggc
      961 gatcccgtag cccttctccg agagcgcagc catcaagccg gaagcgacgg tggtcttgcc
     1021 gacgccggag tgggtgcccg ccacaactac acgaggtagc gaagctccta ccactcgatc
     1081 cctttctggc cccggtagcc ctcgtccatc gggtgcttca ccttattgac ctcgctgacg
     1141 aggtcggaga tctcgaccag ctccggcggc gcgtcccgtc cagtaatgat gacgtgctgg
     1201 aagccggggc ggttcttgag cacctcgatg acctcgcccg tgtccaccca cccgaacttc
     1261 atcggatatg tgaactcgtc cagcaggagc atgtcgtatg tctcgtcggc caggcgacgc
     1321 ttgacctctt cccagccctc gcgggccttg tccgcgctct ctaccaggtc ctttgaggtc
     1381 cacgtccagc catcacccat cttgaaccag tctatgttgc ccagggtctc ggcggccttc
     1441 tgttcgccaa ccttccactt cccgctcttg acgaactggt ataccccgat gcggtagcct
     1501 cgcgcccatc ctcgcagcat gatgccgaac gaagccgtgg acttcccctt gccgtgtccg
     1561 gtgttgacga tgatgagcgg tcgttcccgg ttggacctct tcctcaaccg ccgctcccgc
     1621 ggttcccgtg attctctaac ctcgctcacg ccacccttct cctctctacc agttccacca
     1681 gcgactctgc ccgcagttct tctaacctga ggcaccgcgc tccgagggcg tccgcgacct
     1741 ctcccgccat cccgagccgc acgtatcctt cctccgtgtc cacaacgaag gccgtggcac
     1801 ccagagatct cagacccgat gccgccacgc gcgggtcggg tcccgcagtt gcccgtccgt
     1861 ccgtcagcag gacgagcagc ggccggcgct cccggtcgcg cagccgttcc cgcttcagca
     1921 cctcggcggc cttctcgagg cccgccgcga gaggcgtgcg gcctccggtc ggcagctctt
     1981 cgaggcggga tgcggcgagg ttcacgctgg atgtcggtgg cagaaggatc tgcgcgcccg
     2041 ttcctctaaa agagatcagg ccgaccttgt cgcggcgctg ataggcgtcg ttcagaagag
     2101 acaggacagc gccttttacg gcggacatac gcttctttgc ggccatcgag ccgctcgcgt
     2161 ctaccaggaa gaggatcagg ttcccttccc gtccctcccg cacgttctcc cgcagatcat
     2221 acgggcgcac cgcgagcccc ggccctctcc gtcccctcgc cttttgatga ggtgccgccg
     2281 cccgaaccgt cgccgccagc gccacgtctt tcgagtttgc cccacgctct ctatctccga
     2341 ccggatgccc aaacgccccc gaaatacggc tccgccgccc gagcggcccg ccgcgtccct
     2401 tctcaggcgc ctccagacgt accggatcga acatctccga agctccgtgc gccttctccc
     2461 cggagcctat ccgcgactcg gaaggttcgg taaaggacac ctctccgttt tgcccgtcac
     2521 tctctggtgg cggcgtatct ccgccgccgg ggtcgtcagg cggttcgggg tcttcggggt
     2581 cggagagggc gttctccagc tcttccggtt cgatgccggg ctgctcgaac ggaccacgcc
     2641 tccggcggtg cgagagggcg aggagggcgg cgcgcttcac gtcttccagg acgacctcgt
     2701 cgcgggcgtc ccaggcggtg agggcgcggg cggtcttcgc ggtcacgatg tcgccacgca
     2761 ggccgtccac gccaagctcg gcgcacagag cggagatcct gtagagggta tcttcggaga
     2821 gatgaacacc gggtagacgc cccctcgccg ccacgaccgt ggccgccagt tctgtgtcgg
     2881 cggcggacca cttggcggcg aactcttcgg gaccggcctc gtagtgcagg cggcggcgga
     2941 cgacctcgac ccgctcggat ggctccgggc tgccggagac ctcgaccgtg ataccgaagc
     3001 ggtccaggag ctgcggccgg agctcaccct cctccgggtt catcgtgccg accaggatga
     3061 agcgtgaagg gtgacgcacg cttacgccct cgcgctcgac gtggttcacg cccatggccg
     3121 ccacgtccag gagaaggtca accagatggt ccgagagcag gttcacctcg tccacgtaca
     3181 ggatgccccg gtgcgccgcc gccagaagac ccggctcgaa cgccttgcgg ccctccgaga
     3241 gcgccttctc gatgtccaga gttcccgcca gccggtccgt gctcgctccc accggcagtt
     3301 ccacgagccg caccggccgc tcctcgacgg gcgcgtccgg cgggtgcggt ccagccgggc
     3361 actcgggatt tggcgcctcc gggtcgcagg agtacaagca accggcgacg acccgtatct
     3421 gcggcagcag cttcgccagc gcccgcaccg ccgtggactt cgcggtaccc ttctcgccgc
     3481 gcaccagcac gccgccgacc tcgggggaga cggcgtttat gagcagcgct gtcttcaggt
     3541 cttcctgtcc gacgatggcg gagaaggggt acgggtgcga gtacgcgctc aagagccact
     3601 ctcctccagc atcccctcgt tctcaaggta cgctcgcttg agcgcgtcca aatcttcggg
     3661 gtcgggctcg gaccagaggc cgcgctcggc ggcttcgagc aggcgttcgc ttatggcgcg
     3721 caaggcccag gggttcgact gctgcatgaa gtcccggacc tgttcatcga gaatgtactt
     3781 gcgcgttacg tcgcggtaca tccagtcctc gacgacgttc gcggttgcgt cgtagccgaa
     3841 gaggtagtcc accgtcgcag aaagctcgaa ggcgcccttg taaccatgac gttgcatcgc
     3901 ggagatccac ttcgggttcg ccacgcggga gcggaagacg cgcttggctt cttcggcgag
     3961 ggtgcgagtc tttacacggg acgggtcggc ggagtctccg atgtaggctt tggggtcgcg
     4021 gccggtgagg gcgcggacgg cggcgatcat gcccccgtgg tactggaagt agtcgtcgga
     4081 gtcgaagagg tcgtgctcgc ggttgtctat gttctttacg gcgacctccg tccggcgcag
     4141 gttcgactcc atcgcctcgc gcgcctccac gccgtcgagc cccttgccgt aggcgtagcc
     4201 gccccagacg gcgtagacct cggccaggtc cgcgtcgtcg cgccagttgc gggcgtccat
     4261 gagcgggagg aggccggccc cgtaggcgcc cggtttggag ccgaagatgc gcgtggtgga
     4321 ccggcggtcg tcggcgccgt cggctttctc ctcactagcg tgctttttga cgaagttcat
     4381 ctcgtcgggt tcgtcgaggg cggcgacggt ggtgaaggct tcgtccatca gggagatcag
     4441 gttagggaaa gcgtcccgga agaagccgct gatcctgacg gtgacgtcca ccctcggcct
     4501 tccaagctcc tccagcggga tgacctccag ccgcgtaacc cgccgcgact cctcgttcca
     4561 tacgggccgc accccgagca gggccagtat ctccgcgatg tcatcgccct gtgtccgcat
     4621 cgccgccgtg ccccacacca cgatcccgac ggtctcggga tgccgtcctt cctcctccag
     4681 gtaacgctgc aacaagttct ccgcgagccc ctgcccgacc tcccacgaga gcgccgacgg
     4741 cagggctttc gggtcaaccg agtagaagtt cctccccgta ggcagcacgt tcaccagccc
     4801 ccgcgtcgga gacccggacg gtcccgccgg cacgtacccg cctgcaaggc cggagagcaa
     4861 attccccatc tcctccggcg tccgcatgag acgcggcacg acctcgtccg ccgcgaaccg
     4921 cagcgaccgc tccacccccg catccgcgac gcccagaacc tcctcgcaga ccgttccggc
     4981 ggcctctgcg tcccagccgc gctcctccat cgccaggagc agggattgca ccgcctcctc
     5041 cagccggtcg agcaggtcag aggccgtggc gaccacgccg gggaaccgct ccgcgagggc
     5101 agcggggact gtaacgcggg cgccgccgtt ctcggacagg gctcgctcgt ccaggccgta
     5161 ggagtctccg acggcgcggc gcaagccgga catttgtccg ctacctattc ggaggataga
     5221 ggcgacgagg tggcgcaggg gttcgccggc gggtggttcg ccgaggatat gcaggccgcc
     5281 ccggatgggg aggtccttta tctcgcagag gtagccgtcc acatgggtga ggaagtcgcc
     5341 gaactcctcg ggctgctcct cgacgccgag gtcgcggtga agctccgcgt cgcgcaggac
     5401 ctcccagatc ctgacccgga tcgccggtag cttcgaggga tccagcgtct ccacctggta
     5461 gtactcgtca aggagctgct ccagcttcgc caggtcgtcg taggtgtcgg cgcgggtcat
     5521 cggcgggatg aggtggtcga tcaccgtcgc gtgcgcccgc cgcttggcct gcgtcccctc
     5581 gccgggatcg ttgacgacga acgggtagaa gagcggcacg tcctttagcg ccgcgtccgg
     5641 ggcgcatgag ggcgagagac ccagcgactt gcccggcagc cactccagag tgccgtgctt
     5701 gccgaggtgg acgacggcat ccgcgcagaa ctcctcgatc agccaccagt acgcggcgag
     5761 gtagtggtgt gtcggcggaa ggtcggggtc gtggtagatc gcaatggggt tctccccgaa
     5821 ccctcgcggc ggctggatgc cgatgaagac gttcccgaag cgcagacccg cgaccacgaa
     5881 ctcatcccca tccacgtaca gctcgcccgg cggcgctccc cagtgttcct cgacaccgct
     5941 ccgcaagtct tccggcagcc gggcgaacca ctccgcgtag cgccgcgcat cgagtcgccc
     6001 gatcgcgccc tctagctgat ccgctgtcag gaactccagg tcgtgccctc cagcggcgat
     6061 gagcgagtgg atgagcgcgt ctccctcttc cggcacgcca ttcactatat atccggcatc
     6121 gcgcatagcg ccgagcagct tgatggcgct cgccggggta tccaggccga cggcgttccc
     6181 gacgcgcgag tgttttgtcg ggtagttgga gagcaggaca gctatccgct tttccgcgtt
     6241 cgggacacgg ctgaggcggg cgaagcgggt agcgagaccg gcgagtcggg cggtgcgttc
     6301 ggggtcggcc tggtagagtg tgagcggcgc gccgaccgac gagtccgctc cgacgcgctc
     6361 cttgaaggag aacgggacgc ccacgatgcg tccgtcgaac tccgggatgg cgacctgcca
     6421 tgcggtgtcg aggggcgaga ggccggcgtc ggcgtgggtc cacgttttgt gggacgacgt
     6481 cgcacatatt ccctgcacca cgggtacgcc gaggtcttcg agagccggga cctcccactc
     6541 cagccagtcc tctggggagc cggccttgcg agcgtcggcg gcgttggatc caccaccggc
     6601 cagaacggtc gttacgaggc agtccacccg tcccttgagc atctccagcg ccggtactcc
     6661 gccatcagcg tcggcccgca gcgagtagca gtagactggc agggcgttcg cttcggcctc
     6721 cgcgagcgcc tcgaccagag tatccacgaa ggcggtgtta ccgctcatcc agtgcgcgcg
     6781 gtagaagacg atgccgacgg tcggacgctc cgggtcgtgc agcgagatca ggtcttccag
     6841 cggagaacct tccggtagtc tcggatggta gacgcccatc tccggcagtg gcgcggcggg
     6901 ctcgaagccg tagccttcca tgaggacggt atcggcgacg aagcggagca ggttctccat
     6961 attgcggacg cccccgtggc gcaggtactc gaaggcctcc agcacggtcc ccgaagggac
     7021 ggtagagacg gcggtaagct ccgcgtcggg ctcggcctcc ccgccgaagg cgagcaaggg
     7081 tatgtgaagc tccgagcatc gccttctaag ttcttccaga ccctccggcc acgcccgccg
     7141 cccgccgagc agacgcacca taacggcccg cgcctccggc agcgcgctct cgaagagatc
     7201 caccgggtct tcgaccgaga cggggttggc gcaccggacc ttggggaagc cttccggcag
     7261 gcgctcggcg gctttcgcgg cggccaggat ctcggtgtcg gcggtggtca ggaagagaag
     7321 cctgtcagcc atcagctctc gctccgctgt ttgccagaac ctgccgccaa gaatgccgcc
     7381 ttgtcagatg cggaaacctt gccgttctct gtggctggtg cccccaggcg gggagtatcg
     7441 ccgtccgggt tccgctgtcc gtcgacgcct gaaagccgag agccaacgag ttgcccgctg
     7501 actaccggca tccggcgcgg cgctccatcg tctatcagcc gcaacaacgc ctcccggtcc
     7561 acgttctccg ctatgaggtc tcccagcttc tctagctgcg cctcgcgggc ggcggcgaag
     7621 gactcgtctc caggggtcca gtcgaggtcg cgttgttcag agatccaacg caggaaggcg
     7681 cggcggaagc cgtcggactc cagtatgccg tgccaggaag ttccgaacgt cgtttcgacg
     7741 gcgcatccct cgtcgaagcc gtcgccggtg aacagggttt cacctccgtg gcggtggact
     7801 ctgccgtggt ggatctcgta cccggagacc tcctcgttac cgaagcccgg cgcagatcct
     7861 tccgggcgcg ccaacacctt ttcctcttcg aagaccgtct ctacgggcag caggcccagc
     7921 ccagcggtct ctccctcacc gctctctata ccgtccgtga tacgttcgcc aagcatctgg
     7981 tatccgccgc agatcccgag tacgggcaag ccgagccttg cacgctcgga gagagcgcgg
     8041 tcgaggccgc gggagcgaag catctccagg tccgccacgg tcgccttcgt gccggggagg
     8101 acggcgagat ctgcttcgag cagctcttcc ggcgactcgg tgaagcgcac ccggacgccc
     8161 ggctcgtggg ccagcgcatc gaggtccgtg aagttgctga tacgcccgag gcgcgccacg
     8221 gccacccgca gaacgtcgcg gccgagcggc ggcttcacgg ggcgcgggac atccagggcg
     8281 agcgagtcct cgccgtcgat cccaattccc cgcacgaaag ggagcgtgcc gaggaacggc
     8341 cgtccggtga gctccttcat ccgggcgagt ccgggcgcga gtaccgccgg gtcgccccgg
     8401 aacttgttga ccaggaagcc tgcgacgagc gcctggtctt ccagcgagag cagggctagc
     8461 gtgccgtaga gagatgcaaa gaggccgccc cggtcgatgt ccccgacgac gaggacgggc
     8521 aggtgcgcgg cgcgggcgag gcccatgttg gcgaggtcgt tctcccgaag gtttatctcc
     8581 gccgggctcc ccgccccttc gcacaccacg acctcgaagc gccgccgcag gtcctccaga
     8641 gacttcagca ccaccggcag caactccctc ttcatcgcgc cgtagctccg ggcgctcgcc
     8701 atcgcccacg gcttgccgcg cacgacgacc tgcgtctcgc gctcggcgga cggtttcagg
     8761 aggatggggt tcatggcggc ttcgggctct atcttcgcgg cggcggcctg ggcggcctgg
     8821 gcgcgtccga tctcagctcc ctcccgcgtg acgtaggagt tgagcgccat gttctgcgcc
     8881 ttgaacggcg cgaccttcac accctcgcgg acgagccagc ggcagatgcc ggccacgacg
     8941 acgctcttcc ccgcgtcgga gtgggtaccg gcgacgagca gggctccgcc agcgccgctc
     9001 aagccacgcc gctcttacgg ttcgcccgct cgcacaaaag accgaagacc accccgagtc
     9061 ccgtccacag gacgagttgg gtccccagag acgagagccg gaagctccac aggagcccgg
     9121 cgggaaagtc tccgggagac gcggcgtccg gcagagtcac gaagagaaac accacgacga
     9181 ccgctagccc gacgccgacc gccagttggc ggggcggggt ctcgatgccg cgctcccgga
     9241 ggaggcgggc ggagatccag gctacaagca aggcaagcaa agacagagcg accatcgtga
     9301 agtaggcgat agtgcggtcg ccgatggtgg cgggatctcc gacggttggc gggttggcgg
     9361 ggtatttgag gaacgggatc aggaccgccc cggcgaatat ggcggccgac agcgagttgc
     9421 tccgagccca gtccgtcgcg gagctcagac gatctcgcaa gacggcgtag acgagcccga
     9481 agatcccacc aaccgtaacc ccgaagagcc ccgtggcgaa gaagagcccg accttctggg
     9541 tcgcgcgaga gaagacctcc tctgaatggc ccccggaggc gtgctccatc tctatggccc
     9601 ggtccaggag cggttccccc acgaacaacg cgaacagtcc cgccaggaga ccggcgagca
     9661 ggccggcggc catgccgcgg cgcaggtaag ccgagaacat gctagagcct agtggcaggg
     9721 aacggcgagc aggtgccgcc cgtcatgggc gaactcgtgc aggtagttga acacgctcgc
     9781 gctccgtccc agtaccggcg ccagaagccc cccgctggcc gacagcagca agaacaaagc
     9841 caggagcaga agggct
//
LOCUS       DASSMB010000115        25472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1292414, whole genome shotgun sequence.
ACCESSION   DASSMB010000115 DASSMB010000000
VERSION     DASSMB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 25472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25472
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1292414"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFJ72_07115"
     CDS             <1..519
                     /locus_tag="VFJ72_07115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7479271.1"
                     /translation="RKGRSGAWKELLGKYERLVYSIPLRYGLSRHDAADIAQLTFTIL
                     IQNLDTLPEDSRLGAWLSTVARRHTWRLLESSRRESASGRLESMDLNGSAVLLGRSDS
                     ETIEHWELSEYLDNGLSQLGKQCQELLRALYFQPERPSYAEISAQLGLPLGSIGPTRA
                     RCLKRLREKLGS"
     gene            669..1298
                     /locus_tag="VFJ72_07120"
     CDS             669..1298
                     /locus_tag="VFJ72_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479272.1"
                     /translation="MRERDEIDFGRLVDWIEGDLAPEEAAEVEERVARADEETRADAE
                     WLRAFRSVSEATVLVSPPPEVHDALVGRFEAYAEDRREPGLHRRLLATLSFDSSLQPA
                     FGVRSAGTRESHRQLIFATYAADVALDTFYRSRDNRVDLEGQVFPAGEDPEHHTFAVQ
                     LLEGESDRGTTATDDLGAFSFRAIQPGAYDLLLDDGEVEIRLAQVDLRT"
     gene            1302..4184
                     /locus_tag="VFJ72_07125"
     CDS             1302..4184
                     /locus_tag="VFJ72_07125"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HET7479273.1"
                     /translation="MDISFEHEVARGLVDALLESPSREEQAALIESSGLLNPGGLDRL
                     LEAAAQLVGSDPAKARELATLCADLAERAGSRIAIPRADYVLVQTHFINGEFDEALQR
                     AKRAREGYLALGEDLEALRTDVGRMAVLLEQGHYTEVLQTGAAVLETLDGRGVLQAPS
                     GEYDAVLLAALVYQNLGGCYEYMGRYDRALESYAAAEERYRALGITDRVGEISSNRGF
                     ILLSLGRGSEALAAHEAAADIFEKAGLTLSYSKALANAGEAHLRLANYAQSLDVFERA
                     RKLLRPLEALTEDCFLIRNIAGAYLDLNLYTEALAAYREAVSMLGTTGMVHDRALALW
                     GMGSALIARLEFNEGEQTINEAAALFSEAGNAPMLSGVMLERASLMAARGEREAALEE
                     AQKALDLVSDTDWPVQAVYAHLRLADLNLPDTGRAEAHLRAARQISDRLALPHLRYRL
                     DERLGHVRRLQGREEEARVLLESAIEEIERLRGTVSQEAVRTSFLRDKTAAYEDLLEL
                     YLDRDDAEGRHGAFAVAERAKSRALVDLLTGVIEKAPSAADGGDEEERLRGLQADLNT
                     VYSQILGGTGGEEGLLRLPDLRRRAVELEQKISRIRLEASANHELFSAALPTETVRER
                     LSGDVTMLSYHVIGDEIVAFVSARGDIQTARRLGTVETMEHLLRGLEMQWDRFRAGPE
                     FVGRHMPLLERSTLRILAALYDDLIRPVREMIEAGAPSYGPRKLVIVPHGLLHNVPFH
                     ALFDGESYMLEEFEISYAPSATVYALCQRRPPRMTGRSLVMGVPDPMIPAVADEARAV
                     AASLPGAELLVDERATIEGLRASAARSSTLHLACHGLFRTDNPMFSSLKLHDGWLTAA
                     DVMSLDLNGALVTLSACESGRSEVFAGDEVIGLTRAFLGAGAATLAVSLWLVQDETTA
                     LLMRSWYEKIGTGRGRAAALRAAQLEIKEESPHPYYWAPFILTGHR"
     gene            4321..5649
                     /locus_tag="VFJ72_07130"
     CDS             4321..5649
                     /locus_tag="VFJ72_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7479274.1"
                     /translation="MPASYSGGETRTARSSSGAAAAAGILWPIRRLALCCLALLALLT
                     ITFAAANPAQALVASKDFNNGEVVVKLSGGVAIDEINADYGSVTVEKLLGSAGIYLLK
                     LPASADTQAVAARMETDPRLIYAEPNFVAESPEGDGRFRAFGLSETKPDYEQYAASSL
                     GLSCARKVSRGAGTTVAVLDTGAQLDHPDLQPNFQGVARYDFVDDDTDPSDHPVGLDS
                     NGNGVADELSGHGTHVAGIVEFVAPDAKIMPLRVLDTEGFGNVFVIAEAISFAGRNGA
                     DVINMSFGTAGRSDLLQDVIKQVSESGAVVVAAAGNSSADTPHYPAAGDDVIATTSVD
                     RFNKKSGFANYGPWVDVAAPGDGIRSTFPTSTNAYWSGTSMAAPFVAGQAALIHSVNP
                     LLTAAGVRGVISSTARPLDANNPDYSGMLGAGSADIGASMRQLRPSTVCG"
     gene            6009..7184
                     /locus_tag="VFJ72_07135"
     CDS             6009..7184
                     /locus_tag="VFJ72_07135"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7479275.1"
                     /translation="MKILMYSHDSYGLGHLRRTLALAESFVARSTGTSVLILTGSTVS
                     GTFRMSPGIDLVKLPSAVKVANGVYEPSRMKVNFDSLKKLRSGLILSTAEAFDPDAFI
                     VDKAPLGMKREVYPTLDFLRRHRPFTLTVLGLRDVMDEPWRIRRNWSESGIAQAIEEL
                     YDTILIYGPREVYDPLPEYGLPESTLERCHYVGYVGRGPALEESGALQCPPGFVLVTA
                     GGGGDGFFLIKSYLDSLRGSEPAFDSIVVTGPMMDDDSRREIERLASGLRVSVTEFRT
                     DMEHLIRQAGAVVAMGGYNTTIELLAARKAALIVPRVEPRLEQLIRAKRLSELGLIEM
                     IHPGDLTPARMREKVEALLARDPASFGQVSVDLSGARRAVGIVRSGIARKRTLALGA"
     gene            7189..8409
                     /locus_tag="VFJ72_07140"
     CDS             7189..8409
                     /locus_tag="VFJ72_07140"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7479276.1"
                     /translation="MKVGYVLKRYPRYSETFVVNEILAHEAAGMEVEIFSLLYPEDGH
                     FQDAISRVRAPVTYLPTKGLKAQDFWTMLAEAGDAAPGLWSSLEDARNEEARYVYQAA
                     MLARETRRRGISHLHAHFATASTTVARLAARFAEVPYTFTAHAKDIFHESVVPEDLRR
                     KLADAAVAITVSDYNLEYLRGAHGPAAERVRRVYNGLDLERFPYSPPDERPPLIVAVG
                     RLVEKKGFADLVSACAVLRDRGLEFRCRILGQGDQEAALRERISRLGLDGRVELLGPR
                     PQREMAEYVRKAAVFAAPCVVGSDGNRDGLPTVLLEAMALGTPCVSTDVTGIPEVLQN
                     GKTGLMVPQRDPEALATAIEWLIRDPELRVELAENARRLIEADFDIHVNAARLRQVFR
                     SAAEAPLTMARQVS"
     gene            8455..9669
                     /locus_tag="VFJ72_07145"
     CDS             8455..9669
                     /locus_tag="VFJ72_07145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7479277.1"
                     /translation="MPVLGRKGASVHVRELVAAFGRAGHSVLVAASLLSKSPWEEPAT
                     LEAQLLHLPPAPETASAFYALREFNETLGIEDSLPGELRRILYNQDMLAQLERRFENH
                     APDFIYERASLYSTAGILLARTLERPLLLELNSPLAVEQAAYRQTGLGELAAKAERWI
                     LSRADAVVAVSEPLREYAISLGAEPERVHVVPNGVNPELFHPDIATRGDRWKPGDGPV
                     LGFVGGLRPWHGVEVLPALLERLAARYPHIRLVVAGDGPLRKDLERDARERGLERNIV
                     FTGTLPHEEVAPLIRRFDIALAPYPRPKHDFYFSPLKVFEYMACGVPVVAAGLGQIPD
                     IVRDGETGLLYPPDEPGALLAACERLLDDPDLRVSLGRAAAKEIHARYTWDRNAERVT
                     DLARSLAPRNER"
     gene            9666..10685
                     /locus_tag="VFJ72_07150"
     CDS             9666..10685
                     /locus_tag="VFJ72_07150"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET7479278.1"
                     /translation="MKTNSLTFPVTDPFGVTNDEAMPSLTAALDPDEAQLRLGAALGP
                     ITLREIRVTRYKPERRCVIEYDVEMERPGTTPEAHTLIGKVRRLRSGKSGYRLLDALW
                     RAGFDADAPDGICVPEPAGVVPELRMWLQRKVPGQAATDLLTTPAGEDLARRIAEAAH
                     KVHSSGVPTKRSHTMRDELRILHERLTEVALAKPRWTGRIERLLAACDRLGAATPEPE
                     PRGIHRDFYADQVLVDGERIYLLDFDLYCAGDPALDIGNFLGHIKEQSLRATGDPGSL
                     AHLEAAMEERFVELSGEAARSAVHSYATLTLARHVHLSTLFPERRAFTGDLLDLCEER
                     LVAGC"
     gene            10769..12514
                     /locus_tag="VFJ72_07155"
     CDS             10769..12514
                     /locus_tag="VFJ72_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479279.1"
                     /translation="MSLFFAALCMLGVTLTELLRPWPLAIVFDYILGDKPLPSYLSPF
                     KDYFEGDKTLVLVAVAVSIILIAVLTGIFAYLQIYVTSRIGNELVYTLRRRLFAHLQR
                     LSLSFHNRSRSGEHLTKVVSDTNTLRDVYTEMALNSVSQSLTFVGMIGIMFFLNWQLA
                     LIVLATIPLLVGTFLYRYRTAKASSKRQRSKEAEIANRVSEVLSTVPLVQAFSREKHE
                     EERFETESGEYLRESIRNARIEAVATRSVTIIGALGTAAVVLYGALQVLGGSISPGDL
                     LVFTSYLQSMYKPLRNMAKFSTKYSKASASAERISEVLEVEPEIKDSPDAVDAPPLKG
                     EVVFDGVSFDYGDANGVLEDISFTVLPGQRVALVGASGAGKSTLVSLMLRLYEPQRGG
                     IFMDGVDIKAYKLKSLRRQIGIVLQDSVLFGTTIRENIAYGKLDASREEIEAAAKAAN
                     AHDFIMSLEDGYETILAERGDTLSGGQRQRISIARAIIRDTPILILDEPMTGLDVESE
                     AIVQDALDRLMAGKTSFLITHDLRAVANVDLVLVLEDGRIVEQGTHGDLIAQSNQYRR
                     LHELKTGTEGTGWSR"
     gene            12502..13887
                     /locus_tag="VFJ72_07160"
     CDS             12502..13887
                     /locus_tag="VFJ72_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7479280.1"
                     /translation="MVQVNVGTLAQDPTLPDLAAALDSQAMLKVFRKHLRPIGDTAYE
                     IQSCRLSRIRYRMSFRCILQYTLSLLDPDTGRQRNLLISGTMYADADAARRRFRKLLV
                     EDLAREIPDSLLIFEPVSFVSDLGMLLQVFPYDRKLPTLPLLAAGPPPDLEPLLVTRL
                     GPGDWRAAAWKTESVRYREQIGAVLRYDGRARNTATGEEALRRFYVKVYRDDTGERTL
                     RALGDIRRICDESGESFTVGTPVAYTGCLRALVLEEAPGASLEDVLLDGDDTVETMRK
                     VARDLAAFNQLYMHPEQRYTLMDQISSLERSARLLRWACPNLSPEIDTIVATVADGLE
                     EVRPRPTHRDLKPDHIFLDGARTVFIDLDSFAGADPVLDPALFLAWLAALPDLLPVPR
                     SRARMASRTFASEYFAHVPKSWHDRLYLHYSGALLEVAQGFFRRQEPGWPTKITALVR
                     EAEASLGGGIW"
     gene            13884..14915
                     /locus_tag="VFJ72_07165"
     CDS             13884..14915
                     /locus_tag="VFJ72_07165"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7479281.1"
                     /translation="MTRTDRIGREQKKAALVETAERTYRNPYLFIVGCARSGTTLLHR
                     IVDAHPLIAITPEMHWISQRLKNLESSTARMIPTEVAVELTEHKRFSQFGIDREAFQS
                     LASSTEPVSYTDFLTGVFELYGETRSRPIVGNKTPAYVRRIPELHALWPHAKFVHIIR
                     DGRDVALSVLNWKKADRTAGRFSTWEEDPISTTALWWERKVRTGREAGSALGPDLYHE
                     IRYEDLVSNTRRECVALCDFLKVPYDDAMVRFHEGKTRTDLPDARKTPKKAWLPITGG
                     LKDWRTDMSAEDVERFEAAAGGLLGELGYERTVPKPSPRAVEQAARIRESFGEDIQGH
                     GLGSPERLA"
     gene            14912..15865
                     /locus_tag="VFJ72_07170"
     CDS             14912..15865
                     /locus_tag="VFJ72_07170"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7479282.1"
                     /translation="MNPFVFIVGCPRSGTTLLRRMIDAHSEVAIIDETRWIAAFFERR
                     NGLTSEGMVTPKLIDELLEYDRFAKLEIGRKELENLLRDGPPISYPDFVAGIFDLYGR
                     RRSKYLVGDKTPRYARRIPELHALWPHAKFVHIIRDGRDVALSVLNWKKADRALGRFS
                     TWGRDSLTTVALWWELHLLLARETGSSLSPELYYEMQYEALISDTTNECKKLCKFLKI
                     PYQDAMLRFHEGRERTDPGLDAKKAWRPVTAGLRDWKTQMSDADMERFEAVAGSLLEE
                     LGYYLASRPGTASLEHAAEIRHSFIKDLASRSHALPENWSS"
     gene            15862..16818
                     /locus_tag="VFJ72_07175"
     CDS             15862..16818
                     /locus_tag="VFJ72_07175"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7479283.1"
                     /translation="MNPVVFIVGCQRSGTTLLRRMVDANPHIAIVHESRWVPGLFEER
                     IGLTPEGYVTPDLIRLLLDNPRFALMEIEREDLEGLLAGDDPVPYPSFMTGVYDLYGK
                     KHGKRLAGDKTPSYVRSIPTLHYLWPEAKFVHIIRDGRDVCLSATNWKSGGELRRRFV
                     PLHDEPVTTTAIWWDWLVRSGREAGETLPPDLYREVRYESLVSSPERECEALCEFLNI
                     PYDDAMVRFHEGKERPKPGRSAKNAWLRVTPGLRDWSTDMPADDIERFESAAGELLDE
                     LDYPRAAPDPGSQALATSTAVREAFARELLSGGDRPPAYWRA"
     gene            complement(16829..18355)
                     /gene="guaA"
                     /locus_tag="VFJ72_07180"
     CDS             complement(16829..18355)
                     /gene="guaA"
                     /locus_tag="VFJ72_07180"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7479284.1"
                     /translation="MILVLDFGGQYSQLIARRVREARVFSEMIPYDTPVEEIIAREPE
                     GIILSGGPNSVYQEGAPRVEERLFDLDVPMLGICYGMQLMALTMGGKVEAVQIREYGR
                     SKIEVTEQKLLFADTPDEQVAWTSHGDAVLAAPDGFQVTARTPSVPIIAFEEPALRGG
                     RFGVQFHPEVRHTEYGMEILKNFLFEACECKPDWTPVNIITDAVEKIQAQVGDAKVIC
                     GLSGGVDSATAAMLVHRAIGDNLTCVFVDHGLLRQNEAEQVVEAFGKESDVPLIHVDA
                     SGRFLDKLANVTDPEAKRKIIGEEFIRTFEEEAGRIEDAKFLVQGTLYSDVIESGTRD
                     AAKIKSHHNVGGLPEVMDLDLVEPLRNLFKDEVRVVAAELGMPERMVWRQPFPGPGLA
                     IRVIGDVTAERLDILRRADFVLQDEIRSAGLYRELWQSFAVLPAIHSVGVMGDARTYA
                     YPVVIRAVTSDDAMTADWARLPYDLLERISNRIINEVPGVNRVALDITSKPPGTIEWE
                     "
     gene            complement(18363..19523)
                     /locus_tag="VFJ72_07185"
     CDS             complement(18363..19523)
                     /locus_tag="VFJ72_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HET7479285.1"
                     /translation="MDIEIGRGKTARRAYGLDELAIVPSRRTRDPEDIDISWSLGNLQ
                     LDLPLLASALDAAVDPVTAGIIGDLGGLAVLNLEGIQTRYEDPGPVFEEIASLPETKA
                     TRVMQELYAEPIKEELIFRRVQEIKDRGVLAAASLTPQRVERYHRAALEAGLDVLVIQ
                     GTVVSAEHVSKTVEPLNLIEFVPSLNVPVVVGGCASYSTALHLMRTGAVGVLVGVGPG
                     RICTTRGVIGVGVPQATAVSDAAAARTRHYMETGEYVNVIADGGMRTGGEIAKAIACG
                     ADAVMLGSAFAKAEEAPGRGYSWGMATFHPTLPRGTRIQTSITGTLEEILIGPAREND
                     GTLNLMGALRTSMATTGYQNIKEFQKAEVMYAPSLATEGKTEQRSQHVGMGK"
     gene            complement(20030..21655)
                     /gene="groL"
                     /locus_tag="VFJ72_07190"
     CDS             complement(20030..21655)
                     /gene="groL"
                     /locus_tag="VFJ72_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563800.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET7479286.1"
                     /translation="MAHKELKFDSDARRALEGGVNKLADAVKVTLGPKGQYVVLDRKF
                     GSPTITNDGVTIAREIELEDIFENQGAQLVKEVATKTNDVAGDGTTTATVLAQVIVRE
                     GLKNVAAGANPVILRAGIQKAVEAAVEAIKGQATEISEKDEISRVGAISARSEEVGNV
                     IAEAIDKVGKDGVVNVEESQTFGMDLEFTEGMQFDKGYLSPYFVTDQERMEAVLDDPY
                     ILIANQKIGNVQDVLPILNAVMQSSRPLLIISEDVEGEALATLIVNKLRGTFNAAAVK
                     APGFGDRRKRMMEDIAILTGGEVITEELGLKLENTQLNQLGRARKVVITKDQTVIVDG
                     AGDADAIKGRINQIKAEVENTDSDFDREKLQERLAKLAGGVAVIKVGAATETELKETK
                     HRVEDALSATRAALEEGIVPGGGVALLHAQENVAAILDSLDGDERTGARIIHRALEEP
                     IRQIAANAGSDGSIVVDQVRTRGGSIGYNALTNEYEDLVAAGVIDPAMVTRSALQNAA
                     SIGSLIVTTEVVVAEPEDEAPMGMPGGGMGGMM"
     gene            complement(22059..23846)
                     /locus_tag="VFJ72_07195"
     CDS             complement(22059..23846)
                     /locus_tag="VFJ72_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HET7479287.1"
                     /translation="MASYLSWTRAAYVFFVLAVLAALLKIGGAGLAQQKILAAESSDP
                     ETTIEISTPENTVAETTTAARPRAEPTPAASPSTEPSPKPIHGVPVKRAVAQEKPASI
                     DKPDKPPVFYRPSGRSAAPSAAGMAPAVTGPVCGDLGNFPKSSKAIFPLSDRYFNSYE
                     DTWGASRPQGGHEGTDLMVPAGTQEYAVTDGTIVPVSGANGHGWNMLGGYTVMLRADY
                     DVGPIEAGDLFYYAHMQKPSALKIGTRVRVGQVIGYAGDTGQGPEVTSGLFPPHLHFG
                     WYDTTGARTALDSGAMNPFPLLEWLRSNGGAVSGGSDAEYCKAPQAGPPMPSTGGGNW
                     SYPTSPGVRPDLDTGSDDGRPSPVVKESETSAERAPGGDKEKPDRQKPDGRPENRPEG
                     DQATEHPAPKPRPPEVTAPDVHLPKPPGAIDPPGTATPRPHKPHEGAKPAADPKSIKE
                     WVDHLIETVTAAPDDARPKDRPGPEDDKRKKHPKKHDKHEKHPKREQPDRPKHQQRSE
                     QGEDPACDDQETGGASGAEPCEETTGSPETTPNTEDTTPAEPNAEKATEPAPEDTVSV
                     APPPDEPDAAAPEQESGPEQTSGEGTTAQ"
     gene            complement(23971..24960)
                     /gene="tsaD"
                     /locus_tag="VFJ72_07200"
     CDS             complement(23971..24960)
                     /gene="tsaD"
                     /locus_tag="VFJ72_07200"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7479288.1"
                     /translation="MILAIETSCDDTCAAVVEPDGRRALSNVVHTQTEHERYGGVVPE
                     VASRAHLERLDGVVERALAEASVTLDDVTRVAVTTRPGLIGALLVGVAGAKGLAYSRG
                     LPLAPVDHLEGHVAAAYLTEPDLEPPFVALIASGGHTALYSVQPEGGMDLLGETLDDA
                     AGEALDKGARMLGLGFPGGPAISKAGAGGDLTRYEFPIGLKERDNLDFSFSGLKTSLL
                     YRLKALDEEQVREEVPHLAASYEGAVVEALVRKLLRAADLHETPAVVVAGGVAANALL
                     RRRLEAECGNRGLRLIIPSPALCTDNAAMIGAAAARSAAVPYPRYLSLNARSV"
     gene            25008..25253
                     /locus_tag="VFJ72_07205"
     CDS             25008..25253
                     /locus_tag="VFJ72_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479289.1"
                     /translation="MRTFSLLFAFIALVMLAMFVIYLIRGPVDPLIATGLFISFLACL
                     APALLLLKPFESWEDTLRRRAARKERQEREAHERGDA"
     gene            complement(25250..>25472)
                     /locus_tag="VFJ72_07210"
     CDS             complement(25250..>25472)
                     /locus_tag="VFJ72_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479290.1"
                     /translation="GNAIQKSYNPEKVGLMLAGLEVPHVHIHLLPIDRLEDMSFEYAE
                     KDPDPAGLDEAAEVIRAALEDLGASGVAR"
BASE COUNT         4446 a         8480 c         8039 g         4507 t
ORIGIN      
        1 cgcaagggcc ggagcggggc gtggaaggag cttctcggca agtacgagcg gctggtctac
       61 tccatcccgc tgcgctacgg gctctcgcgc cacgacgcgg cggacatcgc gcagctcacg
      121 tttaccatcc tgatccagaa cctcgacacc ctccccgagg acagccggct gggagcctgg
      181 ctctcgaccg tcgcccgccg gcatacctgg cgcctccttg agagcagtcg ccgcgagagc
      241 gcgagcgggc gcctggagag catggacctg aacggcagcg cggtcctcct cggcaggagc
      301 gactccgaga cgatagagca ctgggagctc tccgagtacc ttgacaacgg cctctcgcaa
      361 ctcggcaaac agtgccagga actgctgcgg gcgctctact tccagccgga gcgtccgtcc
      421 tacgcggaga tctccgcgca gctcggcctc ccgctgggca gcatcggccc cacccgcgcc
      481 cgctgtctca aacgcctgcg ggagaaactg ggttcttagg cagccgccgc gcgtttgcgt
      541 aggtttattt acgttatacg ttgtttggac caggtcggat gtatttttcc cgccgcgcgc
      601 agcatctata ctatgtaggc tgcggaggcg ctcggagcgc ggtgaggtgt agcggaggcg
      661 tggagaatat gagagagcga gacgagatag acttcggccg gctggtcgac tggatcgagg
      721 gtgacctcgc cccggaggag gccgcagaag tcgaggagcg cgtcgcccgc gctgacgaag
      781 agacccgggc cgacgccgag tggttgcggg ctttccgtag cgtgagcgag gctaccgtgc
      841 tggtctctcc gcccccagag gtccacgatg cgctcgtagg acggttcgag gcgtacgcgg
      901 aggaccgccg agagcccggt ctccatcgac gcctgctcgc aacgctgtcg ttcgacagca
      961 gcctgcaacc ggctttcggc gtgcgttccg ccggcacgcg ggagtctcac cggcagttga
     1021 tctttgccac atatgccgcg gatgtcgcgt tggacacttt ctaccgttcc cgcgataacc
     1081 gggtcgacct cgaagggcag gtcttcccgg cgggagaaga ccccgagcat catacgttcg
     1141 cggtccagct cctcgaaggg gagtcggatc gcgggacgac agccacagac gacctcggtg
     1201 ctttcagctt ccgggcgatc caaccgggcg cctacgatct tctgctggac gacggggagg
     1261 tagagatacg actagctcag gtggacttgc ggacgtgaga ggtggacata tccttcgaac
     1321 atgaggtcgc gcgcggcctt gtggacgctc tgctcgaaag cccctcccga gaagaacagg
     1381 ccgcgctgat cgagtcctcc ggcctgctca accccggcgg actcgaccgg ctgctcgaag
     1441 ccgcggcgca actggtcggg agcgaccccg cgaaggcccg ggaactcgcc accctgtgcg
     1501 ccgacctagc ggagcgggcc ggttccagga tcgccatccc ccgcgcggac tacgtgctcg
     1561 tacagacaca cttcatcaac ggggagttcg acgaggcgct gcaacgggcg aagcgcgctc
     1621 gcgagggcta tctcgcgctt ggggaggacc tggaagccct gaggaccgac gtgggccgaa
     1681 tggcagtcct gctggagcag ggccactata cggaggttct ccagacgggc gccgccgtcc
     1741 tagaaactct ggatggtcgc ggcgtgctcc aggcgccttc cggcgagtac gatgccgtcc
     1801 tcctcgccgc tctcgtctac cagaacctcg gggggtgcta cgagtacatg ggccgctatg
     1861 accgggcgct cgagtcctac gccgccgcgg aggaacgcta ccgggcgctc ggcattaccg
     1921 atcgggtcgg ggagattagc agtaaccgcg gcttcatcct cctctcgctc ggacgcggta
     1981 gcgaggcgct ggcggcccac gaggccgctg ccgacatctt cgagaaggca gggctgaccc
     2041 tctcctacag caaggcgctc gccaatgcgg gcgaagcgca tttacggctc gccaactacg
     2101 ctcagagcct cgatgtgttt gagcgcgctc gcaagctgct tcgtccactt gaggcgctca
     2161 ccgaagactg cttccttatc cggaacatag ccggcgctta cctggaccta aacctgtaca
     2221 ctgaagcgct ggcggcatac agagaggctg tctccatgct cggtaccacg ggcatggtcc
     2281 acgaccgcgc gctcgcgctc tggggaatgg ggtcggcgct catcgcccgt ctggagttca
     2341 atgaaggcga gcaaacgatt aacgaggctg cggcgttgtt ctccgaggcg ggcaacgcgc
     2401 caatgctctc gggcgtgatg cttgagcgcg cctctctgat ggctgctcgc ggagaacgcg
     2461 aggccgccct ggaggaggcg caaaaggcgc tggacctcgt ctccgatacg gactggccgg
     2521 ttcaggccgt ctacgcgcat ctccgtctgg cagacctcaa cctgccggat accggacgcg
     2581 ccgaggcgca tcttcgggcc gcgcggcaga tctctgaccg tctggcgctg ccgcacctgc
     2641 gctaccggtt ggacgagcgg ctcggtcacg tgcggcgcct ccagggccgg gaggaggagg
     2701 cgcgggtact tctggagtcg gcgatagaag agatagagcg cttgcgcggc acggtctctc
     2761 aggaggcggt gcggacctcg ttcctgcggg acaagaccgc tgcatacgag gacctgctgg
     2821 agctatacct cgaccgggac gatgcggaag ggcggcatgg agccttcgct gttgcggagc
     2881 gggccaagtc ccgggcgctc gtagatctcc tgaccggcgt tatcgagaag gcgccgtcgg
     2941 ctgcggacgg aggagatgag gaggagcggc tgcgcggcct gcaagcggac ctcaacacag
     3001 tctacagcca gatcctcggc gggaccggcg gtgaggaagg attgctgcgt ctgccggatc
     3061 tccggcggcg ggcggtagag ttggaacaga agatcagccg catacgcctc gaagcatcgg
     3121 ccaaccacga gctcttctcc gccgcactcc cgactgagac cgtgagagag cggctgtccg
     3181 gcgacgtcac gatgctctcc taccacgtga tcggcgatga gatcgtcgcc ttcgtgagcg
     3241 cgcgcggcga catccagacc gcccggcgcc tcggcaccgt cgagacgatg gagcacctcc
     3301 tgcggggtct ggagatgcaa tgggaccgct tccgggccgg accggagttc gtcggccgcc
     3361 acatgcccct cctcgaacgc tctaccctgc gaatactcgc cgccctctac gacgacctga
     3421 tcagacccgt cagagaaatg atagaagctg gcgccccctc ctacggaccg cgcaagctcg
     3481 tcatcgtgcc gcacggtctg ctgcacaacg tcccgtttca cgccctcttc gacggcgaga
     3541 gctacatgct ggaggagttc gagatttcct acgcgccgag cgccacggtc tatgctctct
     3601 gccagcgacg gccccccaga atgaccggcc gctccctcgt aatgggcgtc ccggacccga
     3661 tgatacccgc cgtcgcagac gaagcccgcg ctgtagccgc aagcctcccc ggagccgagc
     3721 tgctggtaga cgaacgggcc actatagagg ggctccgggc gtcggcggct cgttccagca
     3781 cgctgcacct ggcctgccac ggcctcttcc gcaccgacaa cccgatgttc tcctcgttga
     3841 agctgcacga cggctggctg accgccgccg acgtaatgag cctcgacctc aacggggcgc
     3901 tggtcacgct gagcgcctgc gagtccgggc gcagcgaggt gttcgctgga gatgaggtca
     3961 tcggcctgac gcgggccttc ctcggggccg gggcggcgac gctcgcggta agtctctggc
     4021 tggtccagga cgagactacg gcgctgctga tgcgaagctg gtacgaaaag atcgggaccg
     4081 gcaggggacg ggcggcggcg cttcgcgccg cccaactgga gattaaggaa gagtcaccgc
     4141 acccatacta ctgggcgcca ttcatcctga ccggccaccg ttagagcctc aagaggcgct
     4201 tctttccgca tctagctcat tctgactagg aaaatctagt tattttgaaa gatgcgctcg
     4261 cgtcggtggt gggctagggt ggttttggac ggttgacgta cgccagtcag gataatacac
     4321 atgccagcta gctacagtgg tggcgaaaca agaaccgcga ggtcatcttc cggggcggcg
     4381 gccgcggccg gcatcctgtg gcctatcagg cggctcgccc tgtgctgtct ggcgctcctg
     4441 gcgctgttga cgataacctt cgccgcggcg aaccccgcac aggctctcgt cgcgagtaag
     4501 gatttcaaca acggcgaggt cgtcgtaaag ctctccggcg gcgttgccat tgacgagatc
     4561 aacgccgact atggctcggt cacggtcgag aagctgctcg gcagcgcggg gatctacctg
     4621 ctcaagctcc ccgcgtcggc tgacacccag gccgtggcgg cgcggatgga gaccgacccg
     4681 cgcctcatct acgccgagcc gaacttcgtc gccgagtccc cggagggcga cgggaggttt
     4741 agggccttcg gtctgtccga gaccaaaccg gactacgagc agtacgccgc cagctcgctc
     4801 ggtctctcct gcgcccgcaa ggtcagccgc ggcgcgggca cgacggtcgc cgtgctggac
     4861 acgggggctc agttggatca ccccgacctc caaccgaact tccagggcgt cgcccgctac
     4921 gacttcgtgg acgatgatac ggacccctcg gaccacccgg taggtttgga cagcaacggt
     4981 aacggcgtcg ccgacgagct atccgggcac gggacccacg tggccgggat cgtcgagttc
     5041 gtggcgccgg acgccaagat catgccgctc cgcgtcctcg acaccgaggg cttcggcaac
     5101 gtcttcgtga tcgccgaagc catctcattc gccgggcgca acggcgcgga cgtcatcaac
     5161 atgagcttcg gcaccgccgg acgctcggac ctcctgcaag acgtcataaa gcaggtttcg
     5221 gagagcggtg ccgtcgtagt agccgccgcc ggtaactctt ccgccgatac cccgcactac
     5281 ccggcggccg gagacgacgt tatcgcgacg acctccgtgg acaggttcaa taagaagtcc
     5341 ggcttcgcca actacggccc gtgggtggac gtggcggcgc ccggcgacgg catccgcagc
     5401 accttcccga cgagcaccaa tgcctactgg agcggcacct ccatggccgc gcccttcgtc
     5461 gccggtcagg ccgctctcat acacagcgtc aacccgctcc tgaccgccgc cggggtgcgg
     5521 ggcgtgatct ccagcaccgc ccgcccgctg gacgcgaaca acccggacta ttccgggatg
     5581 ctcggcgctg gcagcgcgga cataggcgcc agcatgcgcc agctacgacc gagtaccgtc
     5641 tgcggctgaa ccggctaaac gcaccgcccg tcccggaacg cagactcaaa atctccgagg
     5701 agccgtcccc ttccggtgcg ccgtggaacg acagagaata aagtcgcctc tccgtcctcc
     5761 cgaacgtatt tttcgacgtc ccactctgca tctactagtt aacgaatatt taatctatat
     5821 actcggagta ccaggaggga tgttgcaagt aaacgaaggt tgtgaggcaa ttactgcccc
     5881 caattcgagc atcggaccat ccgatcctgg agcgtagacg tattgtttaa cacagagtta
     5941 acggagggag taactttaat ataccgaaca ttcgatagga gtcgcttgat agctggaggg
     6001 gatgcgactt gaagatactc atgtactcgc acgactcgta cgggctgggc cacctgcggc
     6061 ggacgctcgc gctcgccgag tccttcgtgg cccgcagcac gggtacgagc gtcctgatcc
     6121 tcacgggctc gaccgtctcg ggcacttttc ggatgtcgcc cggcatagac ctcgtgaagc
     6181 tgccctcagc ggtcaaggtg gcgaacggcg tgtatgagcc cagcaggatg aaagtcaact
     6241 tcgactccct aaagaagctg cgctctggtc tgatcctgag caccgccgaa gcgttcgacc
     6301 ccgacgcttt catcgtagat aaagcgccgc tcgggatgaa acgcgaggtc taccccaccc
     6361 tggacttcct gcgccggcat cggcccttca cactcacggt gctgggtctg cgggacgtga
     6421 tggacgagcc gtggcggata cgccgcaact ggagtgagag cggcatcgcc caggctatcg
     6481 aggagctgta cgacaccatc ctgatctacg gtccccgcga agtctacgac ccgctgccgg
     6541 agtacggtct cccggagtcc accctcgaac ggtgccacta cgtgggctac gtcggacgcg
     6601 gccccgcgct ggaggagagc ggcgctcttc aatgcccacc ggggttcgtg ctcgttaccg
     6661 ccggcggagg cggcgacgga ttcttcctca tcaagagcta cctggatagc ttgcggggga
     6721 gcgagccggc cttcgactcc atcgttgtaa cgggtccgat gatggacgac gactccagga
     6781 gggagataga gcggctcgcg agcgggcttc gagtgagtgt gacggagttc cggacggaca
     6841 tggagcatct catccgtcag gccggggcgg tggtcgcgat gggcggctac aacacaacca
     6901 tcgagttgct ggcggcgcgc aaggccgccc tgatcgtgcc gcgggttgag ccccggctcg
     6961 aacagctcat ccgggccaag cggctctcgg agctcggcct gatcgagatg atccaccccg
     7021 gagacctcac cccggcccgg atgcgggaga aggtggaggc tctgctcgct cgcgacccgg
     7081 cctccttcgg acaggtatcg gtggacctct ccggcgcccg gcgggccgtg gggatcgtcc
     7141 gctcggggat cgcgaggaag cgcacgctgg cgctcggggc gtagcggcgt gaaggtcggc
     7201 tacgtcctca agcggtatcc gcgctactcc gagacgttcg tcgtcaacga gatactcgcc
     7261 cacgaagccg ccgggatgga ggtcgagatc ttctctctcc tctacccgga agacggtcat
     7321 ttccaggacg ccatctcgcg ggtgcgcgcg cccgtcacct acctccccac gaagggcctg
     7381 aaagcccagg acttctggac gatgctcgcc gaggctggcg acgccgctcc tggcctgtgg
     7441 tcttccctgg aggacgcgcg gaacgaggag gcgcgctacg tataccaggc ggccatgctc
     7501 gcgcgcgaga cccggaggcg cggcatctcc cacctgcacg cccacttcgc caccgcatcc
     7561 accaccgtgg cgcggctcgc ggcccgtttc gcggaggtcc catacacctt caccgcccac
     7621 gccaaggaca tcttccacga gagcgtcgtc cccgaagacc tgcggcgcaa gctggccgac
     7681 gccgccgtag cgatcacggt cagcgactat aacctggagt acctgcgtgg agcccacggc
     7741 ccggcggcgg agcgggtgcg gcgcgtctac aacggcctcg acctggagag attcccctac
     7801 agcccaccgg atgaacggcc acctctcata gtcgcggtcg ggcgtctcgt ggagaagaag
     7861 gggttcgcgg acctcgtttc agcctgcgcg gtcctgagag atcgcggctt ggagttccga
     7921 tgcaggatcc tcggccaggg agaccaggaa gcggcgctca gggaacgaat ctcccgtctc
     7981 ggtctcgacg ggcgggtaga gttgctcggt ccccggccac agcgggagat ggcggagtac
     8041 gtccggaagg cggcggtctt cgccgcgccg tgcgtcgtag gctcggacgg caaccgcgac
     8101 ggcctgccga cggtgctcct ggaagcgatg gccctcggga cgccgtgcgt ctccaccgac
     8161 gtgaccggca tcccggaggt gctgcaaaac ggaaagaccg gcctgatggt cccccagcgc
     8221 gaccccgagg cgctcgccac ggccatcgag tggctgatcc gcgacccgga gcttcgggtc
     8281 gagctggcgg aaaacgcgcg gcgcttgatc gaggccgact tcgatatcca cgtgaacgcc
     8341 gcccgtctgc ggcaagtctt ccggtccgcc gcggaggcgc cgctcacgat ggcccggcag
     8401 gtgtcctgag gtgaagatcc tctgaagatc ctctatatat gcgccgacct cggcatcccg
     8461 gttctcggac ggaagggagc ctcggtacac gtgcgcgagc tggtcgcggc tttcgggcgg
     8521 gccggccact ccgttctggt cgcggcctcg ctcctgagca agtctccttg ggaggagccg
     8581 gcgacactgg aggcccaact actccacctc ccgcccgctc ccgagactgc ctccgcgttc
     8641 tacgcgctca gggagttcaa cgaaacgctc ggcattgagg actcgctgcc gggagagttg
     8701 cgccgcatcc tgtataacca ggacatgctg gcccagcttg agcgaaggtt cgagaaccac
     8761 gccccggact tcatctacga gcgggcctca ctgtactcga ccgccggaat cctcctggcg
     8821 cgaacgctgg agcggcccct gctcctggaa ctgaactcac cgctggccgt ggagcaggcc
     8881 gcctaccggc agacgggcct cggagagctg gccgcgaagg cggaacgctg gatactctcg
     8941 cgcgccgacg cggtggtcgc cgtctcggaa ccgttgcgcg agtacgcgat ctcgctcggc
     9001 gcggagccgg agcgggttca cgtggtccct aacggggtga accccgagct gttccatccc
     9061 gacatcgcta cacggggaga ccgttggaag ccaggcgatg gtccggtact tggcttcgtc
     9121 ggaggtctga gaccctggca cggtgtcgag gtactgccgg cgctcttaga acgtctggcc
     9181 gcccgctacc ctcatattcg tctggtggtc gccggcgacg ggccgctgcg gaaggacctg
     9241 gagcgcgacg cgcgggagcg gggtctcgaa cggaatatcg tcttcaccgg aacgcttccg
     9301 cacgaggagg tagcgcccct gatccggcgc ttcgacatcg cccttgcccc ctatccccgc
     9361 ccgaagcacg acttctactt ctcgccgctc aaggtcttcg agtacatggc ctgcggcgtc
     9421 cccgtagtcg cggcggggct cggccagatc ccggacatag tgcgggatgg agagacgggt
     9481 ctcctctacc cgccggacga gccgggcgcg ctcctcgccg cttgcgaacg tctgctcgac
     9541 gacccggatc tgcgagtgag cctgggccgg gccgcggcga aagagataca cgcccgctac
     9601 acctgggacc gcaacgctga gcgcgtgacg gatctcgccc gctcactggc accccggaac
     9661 gagcgatgaa gaccaactcc ttgactttcc ccgtgaccga cccgttcggt gtcaccaacg
     9721 atgaagcgat gccctccctc accgcggccc tggaccccga tgaggcgcaa ctgcggctag
     9781 gcgccgccct cggccccatc acgttgcggg agatacgggt cacccggtac aaaccggagc
     9841 gccggtgcgt gatcgagtac gacgtggaga tggagcggcc tggaacgacg ccagaggcgc
     9901 ataccctgat cggcaaggtg cggaggctgc gttccggcaa gtccggctac cggctcctcg
     9961 acgcgctatg gagggcgggc ttcgacgcgg acgccccgga cggcatctgc gtccccgagc
    10021 cagccggagt ggtgccggag cttcgcatgt ggctgcaacg caaggtgccg ggacaggccg
    10081 ccacggactt gctgacgaca ccggcgggcg aggacctggc gcgccgcatc gccgaggccg
    10141 cccacaaggt ccacagctca ggagtcccga ccaagcgctc ccacacgatg agggacgagc
    10201 ttcgtatcct gcacgagcgg ctgacggagg tcgcgctcgc gaagcctcgg tggaccggac
    10261 gcatcgaacg gctgctcgcc gcgtgcgacc ggctcggggc ggcgacaccg gagccagagc
    10321 ctcgcggcat ccaccgggac ttctacgcgg atcaggtcct ggtcgacgga gaacgcatct
    10381 acttgctgga cttcgacctg tactgcgccg gagaccccgc cctggacatc ggcaacttcc
    10441 tgggccacat caaggagcag agcctgcgag ctaccggcga ccccggttcg ctcgcccacc
    10501 tggaagcggc gatggaggag cggttcgtgg agctatccgg tgaggcggcg cgctccgccg
    10561 tccactcgta cgccacgctc acgctggcgc ggcacgtcca cctgagcaca ctcttcccgg
    10621 agcgccgcgc gttcaccgga gacctgctcg atctgtgcga ggagcggctg gtggccggat
    10681 gctaggcaag atccggaaag gcgcatcaag gccgagatac cgctcgcagt tcgcggcggt
    10741 ggtgctggat tacctccgcg gagcaaagtt gagcctgttc ttcgccgccc tttgcatgct
    10801 cggcgtgacg ctcacggagc tactacggcc ctggcccctg gcgatagtct tcgactacat
    10861 cctgggcgac aagccgctgc cttcatatct ctcacccttc aaggactact tcgaaggcga
    10921 caagacgctg gtgctggtgg cggtcgcggt ctccatcatc ctgatcgcgg ttctgaccgg
    10981 aatcttcgct tacctccaga tctacgtgac ctcgcgcatc ggcaacgaat tggtctacac
    11041 gctgcgcagg aggctcttcg cccatctcca gaggctctcc ctctccttcc acaaccgctc
    11101 ccggtccggc gaacacctga ccaaggtcgt gagcgacaca aacacgttga gagacgtgta
    11161 tacggagatg gcgctcaaca gcgtctcgca gtcgctgacg ttcgtcggga tgatcgggat
    11221 aatgttcttc ctcaactggc aactggccct gatagtgctg gcgacgatcc cacttctcgt
    11281 gggcactttc ctctaccgct accggaccgc caaggcgtcc tccaagcggc agcgaagcaa
    11341 agaagcggag attgccaaca gggtcagcga ggtgctctcg acggtccctc tggtgcaggc
    11401 tttcagccgc gagaagcacg aggaggagcg gttcgagacc gagagcggcg agtacctcag
    11461 agagagcatc cgaaacgccc gcatcgaagc tgtcgcgacc cggagcgtga ctatcatagg
    11521 cgcccttggc acggcggccg tggtcctcta cggggccctg caggtgctgg gaggcagcat
    11581 ctcccccggc gacctcctgg ttttcacgtc ttaccttcag agcatgtaca agccgctgcg
    11641 gaacatggcg aagttctcga cgaagtactc caaagcctcg gccagcgccg agcggatctc
    11701 cgaggtcctg gaggtcgagc cggagataaa ggacagccca gatgccgtgg acgccccgcc
    11761 cttaaaaggg gaagtcgtct tcgacggggt ctccttcgac tacggcgacg cgaacggcgt
    11821 cctggaggac atctccttca ccgtcctgcc cggccagcgg gtagcgctcg taggcgcttc
    11881 gggagccgga aaatccacgc tcgtcagcct catgctccgc ctgtacgagc ctcagcgcgg
    11941 cggaatcttc atggacggcg ttgacataaa ggcctacaag ctcaagtccc ttagacgcca
    12001 gatcgggata gtcttgcagg actcggtcct cttcggcacg accatccggg agaacatcgc
    12061 ctacggcaag ttagacgcga gccgggaaga gatcgaagcc gcagcgaagg ccgccaacgc
    12121 ccacgacttt atcatgagcc tcgaagacgg ttacgagacc atcctcgcgg agcggggcga
    12181 caccctctcc ggcggacagc ggcagaggat ctccatcgcc cgcgccatca tccgcgatac
    12241 tccgatccta atcctggacg agcccatgac agggctcgac gtggagagcg aggccatagt
    12301 ccaggatgcc ctcgaccgcc tcatggccgg gaagacctcg ttccttatca cccacgacct
    12361 ccgtgcggtg gcgaacgtgg atctcgtcct cgtcctggag gatggccgga tcgtcgagca
    12421 gggaacccac ggcgacctca tagcccagag caaccagtac cgccggctcc acgagctaaa
    12481 gacgggcacg gaagggaccg gatggtccag gtgaacgtcg ggacattggc gcaggacccg
    12541 acccttcccg acctcgctgc cgctctggac tctcaggcga tgctgaaagt attccgaaag
    12601 catctgcggc ccatcggaga tacggcttac gagatccaat cctgccgact ctcccgaatc
    12661 cggtaccgta tgtccttcag gtgcatactg caatacacgc tgagcctcct ggaccccgat
    12721 accggccgcc agcggaacct cctgatctcc ggcacgatgt acgcggatgc cgacgcagcc
    12781 aggcggcgct ttcgcaaact ccttgtagag gacctggcgc gtgagatacc ggactctcta
    12841 ctcatcttcg agccggtgtc gttcgtctcc gatctcggta tgctcttgca ggtctttccc
    12901 tacgaccgca agctccctac actccctctt ctggcagcgg gaccgccgcc ggatctcgaa
    12961 cctcttctag taacaaggct cggtcccgga gactggcgcg cggcggcgtg gaaaacggaa
    13021 tccgtgcggt accgggagca gatcggggcc gtcctgagat acgacggacg agcccgaaat
    13081 acggcaaccg gcgaggaagc gctcagaaga ttctacgtga aggtctaccg cgacgacacc
    13141 ggggagcgga ctctccgggc actcggagac atccggcgca tctgtgatga aagcggagag
    13201 agcttcactg tgggaacccc ggtcgcatat acgggctgtc tccgcgccct ggtcctggaa
    13261 gaggctcccg gagcctcgct cgaagacgta ctcctggacg gcgacgacac ggtcgagacg
    13321 atgcggaagg tcgcccgcga tctggcggct ttcaaccaac tctacatgca cccggaacaa
    13381 cggtacacgc tcatggacca gatctcctct ctagagaggt cagccagact tctcaggtgg
    13441 gcctgcccca acctgagccc ggagatcgac acgatagtcg ccaccgtcgc cgatggtctg
    13501 gaagaagtcc ggccgcgtcc gacgcaccgg gacctgaagc ccgatcacat cttcctcgac
    13561 ggtgcccgga cggtcttcat agacctcgac tcgttcgccg gggcggaccc ggtactggac
    13621 ccggccctat tcctggcctg gctcgccgcc ctgccggacc tgctgcccgt tccccggagc
    13681 cgcgcccgga tggcctcgcg gacgttcgcc agcgaatact tcgcccacgt cccgaaatcc
    13741 tggcacgaca ggctgtacct gcactactct ggcgcactac tcgaagtggc ccagggcttc
    13801 ttccgccgcc aggagccggg ctggcccacg aagatcacag ccctcgtgcg ggaagccgag
    13861 gcatcgctcg gaggtggtat ctggtgacac gaacggatag gatcgggcgg gaacagaaga
    13921 aagccgctct cgtagagact gcggagcgaa cttatcgcaa cccctacctt ttcatagtgg
    13981 gatgtgcacg ttccggcacc acgttattgc accgaatagt ggatgctcac ccgctcatag
    14041 ccatcacccc ggagatgcac tggatctctc agcgcctgaa aaacctagag agttctaccg
    14101 cgagaatgat ccccacggag gtggccgtcg aactgaccga acacaagaga ttttcccagt
    14161 ttggcatcga ccgggaagcc ttccagagcc tggcaagttc cacagaaccc gtatcgtata
    14221 cagacttcct gaccggcgtc tttgagctgt acggagagac caggtccagg cctatagtcg
    14281 gaaataagac ccccgcctac gtgcggcgca tacccgagct gcacgccctc tggccccacg
    14341 cgaagttcgt ccacatcatc cgagatggcc gcgacgttgc cctctccgtt ctgaactgga
    14401 agaaagcgga tcgcaccgcc ggccgcttct caacttggga agaggatcct atctctacga
    14461 cagccctctg gtgggagcgc aaggtgcgaa cgggccgcga ggccggaagc gctctcggcc
    14521 cggaccttta tcacgagata cgatatgaag acctcgtaag caatactcgc cgggaatgcg
    14581 ttgccctctg tgatttcctc aaggtgcctt acgatgatgc aatggtccgc ttccacgaag
    14641 gcaagacgag gaccgatctt cccgacgcca ggaaaactcc taagaaggcc tggttgccaa
    14701 tcaccggcgg tttgaaagac tggcgaactg atatgtccgc cgaagatgtc gaaaggttcg
    14761 aagcggcagc aggcgggctt ctaggagagc tcggttacga gcggaccgtt ccgaaaccga
    14821 gcccgagagc agtggagcaa gcggccagga tcagggagtc gttcggcgaa gacatccagg
    14881 gccatggctt aggatcgccg gagcgcctag cttgaacccg ttcgtgttca tagtcgggtg
    14941 tccaaggtcc ggaacaacct tactgcggcg gatgatcgat gcccactccg aagtggcgat
    15001 catagacgag acgcgctgga tcgccgcctt cttcgaaaga cgaaatggtc taacgtcaga
    15061 aggcatggtt accccgaaac tgatcgatga actgcttgaa tacgataggt ttgccaagtt
    15121 ggagatcgga cgaaaggaac tcgaaaactt gctcagggac ggcccaccca tctcatatcc
    15181 cgacttcgtc gcggggatct ttgacttgta cgggcggcgg cggagcaagt atctcgtagg
    15241 agacaagact cctcgctacg cgcggcgcat acccgagctg cacgccctct ggccccacgc
    15301 gaagttcgtc cacatcatcc gagatggccg cgacgttgcc ctctccgttc tgaactggaa
    15361 gaaagcggat cgcgcactcg gccgcttctc aacttggggc agagattcct taacgactgt
    15421 ggctctttgg tgggagttgc acctgttgct ggcccgcgag accggaagtt cactcagtcc
    15481 agagctttat tacgagatgc aatacgaagc actcatctcc gacactacga acgagtgcaa
    15541 aaagctctgt aaattcctga agatacctta ccaggatgcc atgcttcgct tccacgaggg
    15601 tagagagcga acggaccccg gtctggacgc gaagaaggcc tggcgacccg tcacggccgg
    15661 tttgagagac tggaaaacgc aaatgtccga cgcggacatg gaaaggttcg aggcggtggc
    15721 tggcagcctc ctcgaagaac tcggctacta cctcgcctct cgtccaggaa cggcatcctt
    15781 ggagcatgcg gccgagatcc gacactcctt catcaaggac ctcgcctctc gtagccatgc
    15841 gttgccggag aactggtctt cgtgaacccg gtagtattca tcgtgggctg ccagaggtcc
    15901 ggcacgacgc tgctccggcg aatggtagac gcaaacccgc acatagcgat agtccacgag
    15961 tcgcgctggg tcccaggcct cttcgaggag cggatcgggt tgaccccgga aggctacgtg
    16021 acgcccgacc tgatccgcct gctcctcgac aaccccaggt tcgcccttat ggagatcgaa
    16081 cgcgaagacc tcgaagggct cctcgcaggt gacgaccccg tcccgtaccc cagcttcatg
    16141 accggcgtct acgatctcta cggcaagaaa cacggtaaac gtctcgcagg agacaagacc
    16201 ccgagctacg tgcggagcat ccccaccctg cactacctct ggccggaggc gaagttcgtc
    16261 cacatcatcc gtgacggccg agacgtctgt ctctccgcca ccaactggaa gagcggcggc
    16321 gagttgcggc ggcgcttcgt acccttgcac gacgaacccg taacgacgac agccatctgg
    16381 tgggactggc tcgtccgctc gggccgcgag gccggcgaaa ccctgccccc ggacctctac
    16441 cgcgaggtgc gctacgagtc actggtctcc agtcccgaaa gagaatgcga ggcgctctgc
    16501 gagttcctga acataccgta tgacgacgcg atggtccgct ttcacgaagg caaggaacgt
    16561 cccaagcccg gccgcagcgc caagaatgcc tggttgcggg taacgccggg gctcagggac
    16621 tggagcaccg atatgccagc ggacgacata gagcgcttcg agtcagccgc cggagagcta
    16681 ctcgacgaac tcgactaccc gcgtgccgcg ccggatcccg gatcacaggc actcgcaacc
    16741 agcacggccg tccgtgaggc gttcgcgcgc gagttactgt cgggcggcga ccggccgccg
    16801 gcctactggc gggcctgaga gaggctccct actcccactc gatggtgccg ggaggtttgc
    16861 tggtgatgtc cagcgccacc cggttcacgc cggggacctc gttgatgatg cggttgctga
    16921 tgcgttccag caggtcgtag ggcagacgcg cccagtccgc cgtcatcgcg tcgtcgctgg
    16981 tgacggcccg gatcacgacc gggtaagcat acgtcctggc gtcgcccatt accccgaccg
    17041 agtgtatagc cggcagcacc gcgaagctct gccacagctc ccggtagagt cccgcgctcc
    17101 ttatctcgtc ctgcaacacg aagtccgccc ggcgcagtat atccagcctc tcggccgtaa
    17161 cgtccccgat aacccggatc gcgagccccg gccccggaaa cggctgccgc cacaccatcc
    17221 gctccggcat cccaagctcc gccgccacca cccgcacctc gtccttgaac aggttccgca
    17281 agggctctac gaggtcgagg tccatcacct ccggcagccc gccgacgttg tggtggctct
    17341 ttatcttcgc cgcgtcgcgc gtaccgctct ctatgacgtc cgagtagagc gtgccctgaa
    17401 cgaggaactt ggcgtcctct atacggccag cctcctcctc gaaggtgcgg atgaactcct
    17461 cgccgatgat cttccgcttc gcctccgggt cggtcacgtt cgccagcttg tccaggaacc
    17521 gccccgacgc atccacgtgg atcaaaggca cgtccgattc cttgccgaac gcctcgacga
    17581 cctgctcagc ctcgttctgc cgcagcaatc cgtggtccac gaagacgcac gtcaggttgt
    17641 ctccgatggc ccggtggacg agcatcgccg ccgtcgccga gtccacgccg cccgagagcc
    17701 cgcagatgac cttcgcgtcg ccgacctgag cctgtatctt ctcgaccgcg tccgtgatga
    17761 tgttgaccgg cgtccagtcc ggcttgcact cgcacgcctc gaagaggaag ttcttgagga
    17821 tctccatccc gtactcggtg tgccgcacct ccgggtggaa ctgcaccccg aagcggcctc
    17881 cgcggagcgc cggctcctcg aaggcgatga tcggcaccga gggcgtgcgg gccgttacct
    17941 ggaacccgtc cggcgcggcc agcaccgcgt cgccgtggct cgtccaggcg acctgctcgt
    18001 ccggggtatc ggcgaagagg agcttctgct ccgtcacctc tatcttagag cggccgtact
    18061 cccggatctg gactgcctcc accttcccgc ccatcgtgag cgccattagc tgcatcccgt
    18121 agcagattcc gagcatcggc acgtccaggt cgaagagccg ctcctcaacg cgtggcgcgc
    18181 cttcctggta gacgctgttc ggaccgccag agaggattat gccctccggc tcgcgggcga
    18241 ttatctcctc tacgggcgtg tcgtagggga tcatctcgga gaacacgcgg gcctcccgga
    18301 cccggcgcgc tatgagttgg gagtactgtc cgccaaagtc gaggaccagg atcaaggcgc
    18361 gactactttc ccatcccgac gtgctgcgag cgctgctctg tcttcccctc ggtagccagc
    18421 gacggcgcgt acatgacctc ggccttctga aactccttga tgttctggta gccggtggtc
    18481 gccatgctgg tcctgagcgc gcccatgagg ttgagcgtcc cgtcgttctc gcgtgcggga
    18541 ccgatgagga tctcctccag cgtcccggtg atgctggtct ggatcctggt cccccgcggc
    18601 agcgtcgggt ggaaggtcgc catcccccac gagtacccgc gtcccggcgc ctcctcggcc
    18661 ttggcgaagg cgctgccgag cataacagcg tccgcgccgc aggctatggc cttggcgatc
    18721 tcaccgccgg tcctcatccc gccgtctgct atgacattga cgtactcgcc ggtctccatg
    18781 taatgccggg tccgggccgc cgccgcgtcc gagacggcgg tcgcctgcgg cacccccacc
    18841 cctatgacgc cgcgcgtcgt gcagatacgt cccggcccga cccccaccag caccccgaca
    18901 gccccggtgc gcatgaggtg cagcgccgtc gagtagctgg cgcagccacc gaccacgacc
    18961 ggcacgttaa gcgacggcac gaactctata aggttcaatg gctcgacggt cttggagacg
    19021 tgctccgccg agacgaccgt gccctggatg acgagcacgt cgaggccggc ttcgagggcg
    19081 gcccggtggt agcgttcgac ccgctgggga gtcaggctcg ccgcggccag caccccccgg
    19141 tcctttatct cctgcacgcg gcggaagatc agttcctcct tgatcggctc ggcgtacagt
    19201 tcctgcataa cgcgggtggc cttcgtctcc ggcaggctcg ctatctcctc gaagaccgga
    19261 ccagggtcct cgtacctggt ctgaatgccc tccaggttca agaccgccag cccgccgagg
    19321 tccccgatga tccccgccgt aaccgggtcc acggcggcgt ccagggcgct cgccagcagc
    19381 ggcaggtcca gttggaggtt ccccagagac caggagatgt ctatgtcctc cgggtcgcgc
    19441 gtcctccggc tgggtactat cgccagctca tccagtccgt aggcccgccg cgcggtcttg
    19501 cctcttccga tctcaatgtc cataagtgcg aaacacttcc ttcctgtaag tccctggcag
    19561 gtagcaagaa gcccacggag aacgttctcc gtgggcttcg ataacctttt ccgctaagcg
    19621 ctgcaaatct ttcttatgct acacctcgct acccgcagcg gtcaagcagt ctttaaatac
    19681 gcctatccgc caacgacacc caacaagtaa agtatcacga cgacgacgag gatgatcagc
    19741 aacacgtaga tcaccgtgtt cagcgaaccc actaactacc tcctcctgaa ggcgcctgga
    19801 actaccggac accgccgtat tggttagacg aactgaagta tcaggatgac cagcagcacg
    19861 atcactagta tggtaatcac cagactcaat cccccgcgca cgttctcacc tctctccctt
    19921 tgtccggttg ctcactagca gcattttata cccttcagaa accccgcgaa acctaaaagc
    19981 ggcgcccctt tcgggacgcc gcctggagaa gctctattgg agagcttcct tacatcatgc
    20041 cgcccatgcc gccgccgggc atacccatag gagcctcgtc ctcgggctcg gccaccacga
    20101 cttcggtggt gacgatgagg ctgccgatag aggccgcgtt ctggagcgcc gaacgggtaa
    20161 ccatcgccgg gtcgatgacg ccggccgcaa ccaggtcctc gtactcgttg gtgagagcgt
    20221 tgtagccgat ggagccgccg cgggtgcgga cctgatccac gactatcgag ccgtcagaac
    20281 cggcgttcgc ggcgatctgg cggatcggct cctccagagc ccggtggatg atcctggccc
    20341 cggtccgctc gtcgccgtcg agcgagtcga gtattgcggc cacgttctcc tgggcgtgca
    20401 agagagccac gccgccgccc ggaacaattc cttcttccag ggccgcgcgg gtcgcggaga
    20461 gcgcgtcctc gacgcggtgc ttggtctcct tgagctccgt ctcggtggcc gcgccgacct
    20521 tgatgacggc aacgccgccg gccagcttgg cgagccgctc ctggagcttc tcgcggtcga
    20581 agtccgagtc ggtgttctcg acctcggcct tgatctggtt gatccggccc tttatcgcat
    20641 cggcgtcacc ggcgccgtcc acgatgacgg tctgatcctt ggtgatgacg accttgcggg
    20701 cgcgcccgag ctggttgagc tgcgtgttct ccagcttgag acccagctcc tcggtgatga
    20761 cctcgccgcc ggtgaggatg gcgatgtctt ccatcatcct cttgcgccgg tctccgaagc
    20821 ccggtgcctt gaccgcagcc gcgttgaacg tgccgcgcag cttgttgacg atcagggtgg
    20881 ctagggcctc gccctccacg tcctcggaga tgatgaggag cgggcggctc gactgcatga
    20941 ccgcgttgag gatcgggagc acgtcctgca cgttgccgat cttctggttg gcgatcagga
    21001 tgtacgggtc gtcgaggacg gcctccatgc gctcctggtc cgtcacgaaa tacgggctca
    21061 ggtagccctt gtcgaactgc atgccctcgg tgaactccag gtccatcccg aaggtctgag
    21121 actcctcgac gttgaccacg ccgtccttgc cgaccttgtc gatggcctcg gcgatgacgt
    21181 tgccgacctc ttcgctacga gcggagatgg cgccgacgcg ggagatctca tccttctcgc
    21241 tgatctcggt cgcctgcccc ttgatggcct cgaccgcggc ctcgacggcc ttctggatgc
    21301 cagcgcgcag gatgaccggg ttggcccccg ccgctacgtt cttgaggccc tcgcgcacga
    21361 tgacctgggc gagcaccgtc gccgtggtcg tgccgtcgcc cgcgacgtcg ttggtcttgg
    21421 tcgctacctc tttgacgagc tgcgcgccct ggttctcgaa gatgtcctct agctcgatct
    21481 cacgagcaat cgtcacaccg tcgttggtga tcgtcggcga accgaacttc ctgtccagca
    21541 ctacgtactg gcccttcggc ccgagcgtca ccttgaccgc gtcagcgagc ttgtttaccc
    21601 cgccctccag cgcccgccgc gcgtcgctgt cgaacttcag ctctttgtgc gccactatcc
    21661 agcctcctct gttacactac agaacctcgc ggcgaatgcc gcttttggca ctctctacgt
    21721 tagagtgcca gcgcgcagag tatagtcttt gccgggttac aattcaaacc atgagcatct
    21781 tccgaacaga tagaattccg agtctgagcc gcatcccgag agagctgggc cgggatgagc
    21841 gtttctgtga gcgctgcgag gcgaacacgc agcacatcct ctacctggtc ccgaagaagg
    21901 ttctactcct ctatgtcagg gatcaccccg agaacgtaca tgcgacctgc ttggtctgcg
    21961 cccggagcac ggtgcttact ggagaggatc gggaccgcat cctcgctgcc gcgtataagt
    22021 cctagccgca ctccgcgatc tctccaagac agattgagct actgagccgt cgtaccttcc
    22081 ccggaagtct gctcagggcc gctctcttgc tctggcgcgg ccgcgtccgg ctcgtcagga
    22141 ggcggcgcga ccgagacggt gtcctccggc gcgggctctg tagctttctc cgcgttgggt
    22201 tccgcgggcg tggtgtcctc cgtgttcggg gtggtctcag gagagccggt cgtctcctcg
    22261 cacggctcgg cgccgctcgc gccgccggtt tcctggtcgt cgcaggcagg gtcttctccc
    22321 tgctcggacc gctgctgatg tttcggcctg tccggctgct ctctcttcgg gtgcttctcg
    22381 tgtttgtcgt gtttcttcgg gtgcttcttg cgcttgtcgt cctcggggcc gggtcggtct
    22441 ttgggacgag cgtcatccgg cgcggcggtg acggtctcga tcaggtggtc gacccactcc
    22501 ttgatggact tcgggtccgc tgcgggcttc gcgccttcgt ggggcttatg aggtcgagga
    22561 gtcgccgttc caggcggatc gatcgcgccg ggaggctttg gaagatggac atcgggagcg
    22621 gtgacctcgg gaggacgcgg cttcggagcc gggtgttcgg ttgcctgatc tccctccggc
    22681 cggttctcgg ggcggccgtc aggcttctgg cgatccggct tctctttgtc gccgcccggc
    22741 gcgcgctccg cggaggtctc gctctccttt acgacgggac tcgggcgtcc gtcgtcggag
    22801 ccggtatcga ggtccggcct tacgccgggc gaggtcgggt agctccagtt tccgccgccc
    22861 gtcgagggca tgggaggtcc ggcctgcggc gccttgcagt attcggcgtc cgagccgccg
    22921 gagacggcgc cgccgttgct cctgagccac tccaggagcg ggaacgggtt catcgcgccg
    22981 gagtcgagtg cggtgcgtgc gccggtcgtg tcgtaccacc cgaagtgcag gtgcggcggg
    23041 aagagcccgc tcgtcacctc cggtccctgc ccggtatcgc ccgcgtaacc gattacctgt
    23101 cccacccgga cgcgagtccc gatcttcagg gcgctcggct tctgcatgtg ggcgtagtag
    23161 aagaggtcgc ccgcctctat gggacctacg tcgtagtccg cccggagcat gaccgtgtag
    23221 ccgcccagca tgttccagcc gtgtccgttc gctccggaga ccggcacgat ggtaccgtcc
    23281 gtaacggcgt actcctgagt tccagccggg accatcaggt cggtcccctc gtggcctccc
    23341 tgcgggcgcg acgctcccca ggtgtcctcg taagagttga agtaccggtc cgagagcggg
    23401 aagatggcct tgctgctctt cgggaagttg cccagatcgc cacagacagg ccccgttacg
    23461 gctggcgcca tgccggcggc ggaaggagcg gcggaacgtc cggacggccg gtagaaaacc
    23521 ggcggcttat ctggcttatc tatggaggca ggcttctcct gcgcgaccgc tctcttcacg
    23581 gggacgccgt gaatgggctt gggggaaggc tccgtagacg gcgaggcggc cggtgtgggc
    23641 tccgcccgtg gtcgggcggc ggtggtcgtc tcggctacgg tgttctccgg agtggagatc
    23701 tcgatagtcg tctccggatc ggacgattca gcggcaagta tcttctgctg ggcgagaccg
    23761 gctccaccga tcttcagtag ggcggcgagg acggccagca cgaagaagac gtaagccgcc
    23821 ctggtccaac tgagatacga tgccaaccct ctcccctcta actccacgag gtccgcactc
    23881 ggcataccga atagggctac actctcacac gcccgcgacc agtcgtcaag agcatgagcc
    23941 gcagtcccgg cggataagag cgagacccgc ctatacgctg cgggcgttga ggctcaggta
    24001 gcgcgggtag ggcacggcag ccgaacgcgc agcggcggcc ccgatcatgg ccgcgttgtc
    24061 cgtacagagg gcgggagaag gaatgatgag cctcaagccc cggttcccgc actctgcctc
    24121 cagccggcgg cgcaggaggg cgttcgccgc gaccccgccg gccacgacta ccgcgggcgt
    24181 ctcgtgcaga tcggcggcgc gcagcagctt gcggactagg gcctcgacga cggcgccctc
    24241 gtagctcgcg gccaggtgcg gaacctcctc ccgaacctgc tcctcgtcca gggctttcag
    24301 gcggtagagc aggctcgtct tgaggcccga gaagctgaag tccaggttgt cgcgctcctt
    24361 gagccctatg gggaactcgt agcgcgtgag gtctcctccg gctccggctt tcgagatggc
    24421 cggtccgccg gggaaaccga ggccgagcat gcgcgctccc ttgtccagag cctcgcccgc
    24481 cgcgtcgtcg agcgtctcgc ccaggagatc catgccgcct tccggctgca cggagtagag
    24541 cgccgtgtgc ccgcccgagg cgatcagggc tacgaacggc ggctccagat ccggctccgt
    24601 gaggtacgcg gcggcgacgt gtccttcgag gtggtcgacc ggcgcgagcg gcagcccgcg
    24661 tgagtaggcc agccccttcg cccccgccac cccaacgagc agggccccta tgagtcccgg
    24721 cctcgtcgtc acggcgacgc gggtaacatc gtccagggta acggaagcct ccgcgagcgc
    24781 ccgttcgacg accccgtcga gccgttcgag gtgggcgcgc gaggcgacct ccgggaccac
    24841 gccgccatag cgttcatgct ccgtctgggt gtggacgacg ttcgagagcg cgcggcgtcc
    24901 gtcaggctcg actacggcag cgcaagtatc gtcgcaggag gtctcgatag cgaggatcat
    24961 ggggtattta taacagccag cggcccgctt gccgctatac ttctcccatg cgcaccttct
    25021 cgctgctgtt cgcgtttatc gcgctggtga tgctggcgat gttcgtcatt tacctgatcc
    25081 ggggtccggt ggaccccctg atcgcgaccg gcctcttcat cagtttcctg gcgtgtctgg
    25141 cgccggcgct cctgctactg aagccgttcg agagctggga agataccctg cgccgccggg
    25201 cggcccgcaa ggagcgacaa gagcgcgagg ctcacgaacg cggcgacgcc taacgcgcga
    25261 cgcccgaggc tccaaggtct tcgagcgccg cgcggataac ctcggcggct tcgtccagcc
    25321 ctgccgggtc cgggtccttc tcggcgtact cgaagctcat gtcttcgagc cggtctatgg
    25381 gcaggaggtg gatgtggacg tgcggcactt cgaggcccgc cagcatcagg ccgaccttct
    25441 cggggttgta cgatttctgg atagcgttgc cg
//
LOCUS       DASSMB010000116        10239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_26121, whole genome shotgun sequence.
ACCESSION   DASSMB010000116 DASSMB010000000
VERSION     DASSMB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26121"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VFJ72_07215"
     CDS             complement(<1..308)
                     /locus_tag="VFJ72_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479291.1"
                     /translation="MSGMLTLGSLDPAFDAASRVEGEPVVLDLRPVSFVEPAGICGLA
                     ALLEFLAPRCEGVDLALSGRDVPAYLERMDFFRHFGGRVKTNADLASLEERQRHNPGT
                     "
     gene            complement(412..1053)
                     /locus_tag="VFJ72_07220"
     CDS             complement(412..1053)
                     /locus_tag="VFJ72_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479292.1"
                     /translation="MPFTLDNYSETRPFLYHLTAQENIDRIKSSGCLESAAVLLNSAE
                     NKEIIRTRRIEKMPVRIDSEQIVLRDQAPLYADNINFTDGWTLGDFVEDLNRRVFFWS
                     GWQHAPISYGENHFARYSEERPVVIRTRFNSLLVKNPGRRPLFCKYNSGAPRQFQGRQ
                     SPRGPGTFLPAEQCPYTCGKVVEVTFIEAVKLPEDTEISFNARGPWKPMFSRT"
     gene            complement(1066..1620)
                     /locus_tag="VFJ72_07225"
     CDS             complement(1066..1620)
                     /locus_tag="VFJ72_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7479293.1"
                     /translation="MKTGGAVIHAEEIQILGGESVTLRPPREADVEVAYNWDRDPELA
                     AWNGRSPISISLSAARRDYLSRWEDRSVKTFIIEAGDPREPIGMATLYSFRHGGCDLG
                     IKIGPKTLRGRGYASEAVNLLVQYAFESLGVKVVRGSTLAHNHRMQRVFEKCGFYEAG
                     DGTLLSRYDNKRYTELFYERRRDG"
     gene            1837..3054
                     /locus_tag="VFJ72_07230"
     CDS             1837..3054
                     /locus_tag="VFJ72_07230"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479294.1"
                     /translation="MARLKGISQLGFTTQAFPRARHSRFEHAIGVYHLTRLTLKRITD
                     SGAYLEDREVQGALAAALLLDAARYPYPQSIEGIALPSIISRRELSRNLIEETEVAKV
                     LRNEWDLEPHNVYRLVARGGTPPRGLTPTEHLIRDILFGSLDVDMLDALVRDSQAAGV
                     PYGIVRVEPLIERLRIVGQDNRALMAVDEEGVGSLQSLVFSRYLIHYNLYGHYALRIP
                     TVMFLRAVQDALQAEAVDPFVLSEKDDAGAFIMVQDSGEAGTSTAVLTKRLAERRLYR
                     RALELDERHPSYASLMRLRDDASWRRRVEEAWSRYLTRYRKGVAGPFDILIDLPETSA
                     LTTGLRIIRQYPLPGERNPATWQHISGMTEDDMIRYHAPLRRIRVVTANDDLTTSVRR
                     HAEELFTIAEEVG"
     gene            3249..3321
                     /locus_tag="VFJ72_07235"
     tRNA            3249..3321
                     /locus_tag="VFJ72_07235"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3282..3284,aa:Lys,seq:ctt)
     gene            3457..3744
                     /locus_tag="VFJ72_07240"
     CDS             3457..3744
                     /locus_tag="VFJ72_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479295.1"
                     /translation="MLTAMTRGIEMVHRRVPQVYVGGRLERDESRAVEVAPMGNPLEA
                     TGLLREGQAVEMLIRRDYRAAALVFSDIASKVSGAERGHYYRSLLLLSDSG"
     gene            4400..5233
                     /locus_tag="VFJ72_07245"
     CDS             4400..5233
                     /locus_tag="VFJ72_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7479296.1"
                     /translation="MTERGPARGEQALAEGLHAARRTSQKRAKRISALKLEVEELRAA
                     RAEDERLLHYLLSDAFDRRGPWVTGVRIDGKVYGRATRHTSPRLEEFFEAFPEASKGR
                     ILEPGSLEGAMTVELARRAREVVSLEGRPQNVERAEFLKGLFGVDNVAFHVTDLEADD
                     LSSYGGFDAVFCCGLLYHLPNPRAFVERAAAAAPNLYLDTQYACPGWELAERDGLRGW
                     VSTEDPQDPQSGLSGTAFWPTLDELHRLLRESGYETVVTLARPPDNRHGPRVRIAASR
                     P"
     gene            complement(5390..6535)
                     /locus_tag="VFJ72_07250"
     CDS             complement(5390..6535)
                     /locus_tag="VFJ72_07250"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006941.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase [NAD+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET7479297.1"
                     /translation="MQADASGPLATVFAAPGRYIQGRGAVGRLGEVLEQLKSERPLIV
                     RDRIVEEIMGDALEGISGATHVEFGGECSPGEIDRVAEEARGAGADAIVGIGGGKTLD
                     TAKAVAHPAGLPLVNVPTIASTDAPTSAVSVVYNEEGEVLEYRLFARNPDAVIVDTEV
                     IVGAPVRFLVAGMGDGLSTYFEADASSRTRAKTLVAGAPTQAALAIARLCYDTLLEHG
                     LAARLAAERGAITPSVEKVIEANTLLSGLGFESGGLAAAHSIHNGLTVLEETHQYWHG
                     EKVAFGVIAMLMLEDRPFEAVAEVVDFCLEVGLPVALDDLGLRDASEEDLRKAAEVAC
                     ADGETIYNEPFDVYPQTVLDAILAADAFGRQRRAVLTGEAELPAVAR"
     gene            6726..7034
                     /locus_tag="VFJ72_07255"
     CDS             6726..7034
                     /locus_tag="VFJ72_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7479298.1"
                     /translation="MPGPTPHYPPEFKREAVRLNRSSEKSIPKMAEELGIASESLRRW
                     IRQHEADVGEREGITTEEREELSRLRRENRILKQEKEVLRKASQRLCLAFFTREDVIR
                     "
     gene            7179..7511
                     /locus_tag="VFJ72_07260"
                     /pseudo
     CDS             7179..7511
                     /locus_tag="VFJ72_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            7515..7901
                     /locus_tag="VFJ72_07265"
     CDS             7515..7901
                     /locus_tag="VFJ72_07265"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET7479299.1"
                     /translation="MESHLRTELVIDALQMAVWRRQPSPGLVHHSAQYTALSFSERLR
                     EVVGITPSMGRTGSALDNAMAESFVSTLKAELVSNLKFSSRQAAKTAIFEYLETVYNS
                     RRLHSALGYRSPAAFEEDRVEGARVA"
     gene            complement(8184..8480)
                     /locus_tag="VFJ72_07270"
     CDS             complement(8184..8480)
                     /locus_tag="VFJ72_07270"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET7479300.1"
                     /translation="MEENAAYAIGHIRVKDEEKWAEYRANVPATLSEWGAEIVFRGKR
                     VEVLSGEHDYDDAVVIRFPDANAMRGWYRSPAYQALIPLREQAADLVIVGYDGS"
     gene            complement(8504..9388)
                     /locus_tag="VFJ72_07275"
     CDS             complement(8504..9388)
                     /locus_tag="VFJ72_07275"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET7479301.1"
                     /translation="MDDQTPLIIEVRVNESADRGDNRALPYGPREVVEEGLRAREAGA
                     SILHWHARDTDGSERANDVTLYREVIEGVREQTDDLLLHPTLGFIATQGDAQGRVRHI
                     LELNEDPKTRIDMVPVDFGAFGADIWDQEAERFLTDDQVIVNRVAYLKELLGVLKEND
                     LYVYTVVWGAGAVRTARNLQKMGLLNPATLWCLGFTGDEVPGGPPPTPANLQTFLEAI
                     PEGDPWTVHCRNGDAMALAAWAITLGGHVSIGLGDYPYSRFGTPRNSDLVKMVADMSR
                     TLGRPVATPAEARKILRL"
     gene            complement(9412..>10239)
                     /locus_tag="VFJ72_07280"
     CDS             complement(9412..>10239)
                     /locus_tag="VFJ72_07280"
                     /inference="COORDINATES: protein motif:HMM:NF028206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET7479302.1"
                     /translation="VFGAAGSMGVRVTNALKDDPEYTMLYVEAGEAGQARLREKGFDP
                     TSQEEAVGEADVVIFAVPDTVVGSLAREIVPSLKSGTMVVTLDPAAAYAGELPEREDI
                     SYFITHPAHPPIYNDETDPEARRDYFGSGKAKQAFVNALMQGPEEDYARGEKIATKLW
                     GPVLRSHRVTVEQMAILEPVLSETVTATCLTVIREAVDEAAERGVPFEAARDFVLGHI
                     NVELAILFDELDWSLSAGAQMAVEEAKKDIFQPDWKKVFEPENIKESVTKITSGGSV"
BASE COUNT         1879 a         3285 c         3221 g         1854 t
ORIGIN      
        1 aaggtgccgg ggttgtgccg ctgccgctcc tccagggagg ccaggtccgc gttagtcttc
       61 acccgcccgc cgaagtgccg gaagaagtcc atccgctcca ggtaggccgg gacgtcgcgg
      121 cccgagagcg ccaggtcaac cccctcgcag cgcggcgcga ggaactcaag gagcgccgcc
      181 agcccgcaga taccggccgg ctccacgaag ctcaccggac ggaggtccag gacgacaggc
      241 tccccctcca cccgcgaggc ggcgtcgaac gccgggtcca ggctaccgag cgtcagcatc
      301 ccgctcatag cgccccggct ccgtacattc aatttcaaaa gttgtgtgtt cactagcctt
      361 attgtagccg gtgtaaagcg acgcctttag tgtgttctgt gcatgcgcga cctaagtacg
      421 agaaaacatt ggtttccatg gccctcttgc attgaaagat atttccgtgt cctcaggcaa
      481 cttcacagcc tcgataaagg ttacttcgac gactttgcca cacgtataag gacattgttc
      541 ggcgggcaga aacgttcctg ggccgcgagg gctttgtctt ccttggaact gtcgtggagc
      601 accagaatta tatttgcaga acaaaggacg tctgcctgga tttttcacaa gcagggaatt
      661 gaaacgagtt ctaataacaa cagggcgttc ctcactatag cgggcaaagt gattctcgcc
      721 atatgaaata ggtgcatgtt gccaaccaga ccaaaagaac acgcggcgat tcaggtcttc
      781 tacgaaatct ccgagagtcc acccatctgt gaagttgatg ttgtctgcat acagtggtgc
      841 ttggtcgcgc aaaacgattt gttctgagtc aatacgcacc ggcatttttt caatccttcg
      901 agttcttata atctccttat tttccgctga attgaggaga acagcagcgc tttcgaggca
      961 tcctgaagac ttaatacgat ctatgttctc ttgagctgta aggtggtaga gaaatggtct
     1021 ggtctcagaa taattgtcga gcgtaaaagg catctggtgc gaggtttatc cgtcccggcg
     1081 gcgttcatag aacagctcgg tgtaacgctt gttgtcatag cggctaagga gggtgccgtc
     1141 gccggcctca tagaagccgc acttctcgaa gacgcgctgc atccggtggt tgtgggcgag
     1201 ggtcgagccg cgcacgacct taacgccgag cgactcgaag gcgtactgta cgagcaggtt
     1261 caccgcttcg ctggcgtagc cgcggccccg gagggtcttc gggcctatct tgatccccag
     1321 atcacagccg ccgtggcgaa aactgtagag ggtagccatc ccaatgggct cgcgggggtc
     1381 cccggcctct atgatgaagg tcttgacact ccggtcttcc cagcgggaca ggtagtcgcg
     1441 ccgcgcggcg gagagggaga tggagatggg cgagcgtccg ttccaggcgg cgagttcggg
     1501 gtcgcggtcc cagttgtaag ctacctccac gtccgcttcg cggggcggac gaagcgtcac
     1561 ggattctccg ccaagtatct ggatctcttc agcgtgaata acggctcctc ctgtcttcaa
     1621 gagacaatct tacacgcacc gccgcccgcc acaagcggcc cgggtcctcc tagaggtctc
     1681 gttttcgcta gaatacgcga aggtcaaaca ccgggagtag cattgagcgg tacggttggg
     1741 gagctcttcg gaggctccgg gaggagaatc cgggacgtag tctgggggga cgtccctata
     1801 gaccgggcgg ttaggaccct gctcgagact gaggccatgg ctcgccttaa aggcatcagc
     1861 cagctcggct ttaccacgca ggctttcccg cgcgcgcgtc actcgcgctt cgagcacgcc
     1921 ataggcgtct accacctgac gcggctcacc ctcaagagga taacggactc cggcgcgtac
     1981 ctggaggacc gggaggtgca gggcgctctg gccgccgcgc tcctgctgga cgcggcccgt
     2041 tacccgtacc cgcaatccat agaaggaata gccctgccaa gcataatctc gcggagggaa
     2101 ctgtcgcgga acctgatcga ggagaccgag gtagcgaagg tcttgcgcaa cgagtgggac
     2161 ctcgaacccc acaacgtcta ccggctcgtc gcccgcggcg gcactcctcc gcgcggcctc
     2221 acccccaccg aacacctcat ccgggacata ctcttcggct ccctggacgt ggacatgctc
     2281 gacgccctgg tccgggactc gcaggccgcg ggagttccct acggcatagt gagggtcgag
     2341 cccctgatcg aacgcctccg catcgtcggt caggataacc gcgccctgat ggcggtggac
     2401 gaggagggcg tcggctccct ccagtctctc gtcttctccc ggtacctgat ccactacaac
     2461 ctctacggcc attacgccct gcgcatcccg accgtgatgt tcctgcgcgc cgtccaggac
     2521 gcccttcaag ccgaggccgt cgaccccttc gtcctctccg agaaggacga tgccggagcc
     2581 ttcatcatgg tccaggactc cggcgaagcc gggacctcca cggccgtcct caccaaacgc
     2641 ctcgccgagc gccgcctcta ccgccgggca ctggaactgg acgagcgtca cccaagctac
     2701 gcgtcgctca tgcgcctgcg cgacgacgct tcctggcgcc gccgggtcga ggaagcctgg
     2761 tcacgttacc tgacccgcta ccggaagggc gtcgccggac ccttcgacat ccttatagac
     2821 ctgccggaga ccagtgcgct cacaaccggt ctgcgcatca tccgccagta tcccctccct
     2881 ggagagcgca acccagcaac ctggcagcac atctccggca tgaccgaaga cgacatgatc
     2941 cgctaccacg ccccactccg ccgcatccgc gtagtcaccg ccaacgacga tctgacaacc
     3001 tccgtgcgcc gccacgcaga ggaactcttt accatcgccg aagaggtggg ctagagcggc
     3061 acccggacaa agaaaaaggg ccggtattca cagaagggtg agaccctttt ccggccctta
     3121 gcggcctcct gaagagaccg ctaggatgat acagatatta tatggacgcc cgaccagcgg
     3181 cgcaagcccc tctcaataaa acttaatctt ttgccgtctc gccgtccgat gctatacttt
     3241 cgcttcatgg gccgctagct caattggcag agcaagagac tcttaatctc taggttccag
     3301 gttcgagtcc tgggcggctc actcggaaag tacctgcaaa tagcgggaaa gcgaaagccc
     3361 cggtttctac gccggggctt tctgacacca gttttgacac cagtttcggc aaatccggcc
     3421 gattctcaac ccaaagcctc atccatcgcg gatgcggtgc tgacggcgat gacgcggggc
     3481 atcgagatgg tgcatcggcg ggtgccccag gtctacgtgg gcgggcgctt ggagcgggac
     3541 gaatcgaggg cggtggaggt cgccccgatg gggaacccgc tggaggcgac cgggctcctg
     3601 cgcgaggggc aggcggtgga gatgctcatc cggcgcgact acagggcggc ggcactggtc
     3661 ttttcggaca tcgcatccaa ggtgagcggc gcggagcgcg gacactacta ccgcagttta
     3721 ctccttctct ctgacagcgg ttagcgtaag tattgatccg ctgctaacct gcgggcatga
     3781 aacgaccact gcggctaccg cccgctggat caacctttgt gagtaccgct gtgagatcgg
     3841 cgtccacgtc cgccgtgatc ctgaccctgg tcatccgata gcctccggta gcttcgtcta
     3901 tcccgtctta ccagcggcga attggtgtca gggtgacacc cttattctgg cggaagacgg
     3961 cacggtgagg tgtatttttg cgaagtgccg gaaggtggcg tgggagatcc gcgtgggaga
     4021 tccgaacttg gcgagggtgt taaggaagac gaggtttctg gatagccttc cgtagggagt
     4081 agcgcgtgcc gtcgctataa ccccggatgg cagagaagcc ggcggcaagg gaagcgcgtt
     4141 agggcgtggt catattcttc aagagcttcg tggacgactc cgcgcccgaa ggggagcccc
     4201 gaggcgagcg ggaggagtac ctgcacgccg aagggatttc tcgcgaccat ctgctacgat
     4261 cagcaccggg ccgcggtcta cggccgcggt ctgcggcggc gggatattcg gagggcgagg
     4321 tgccacgctt ctcgaacagc aggactctct cttctggcaa gctggggatc cccctcgggc
     4381 tgagtgggaa ggacgggtgg tgaccgaacg cggtcccgcg cggggcgagc aggcgctcgc
     4441 ggagggcctc cacgcggccc ggaggacctc gcagaagcgg gcgaagagga tctccgcgct
     4501 gaagctggag gtcgaagagc taagggctgc ccgagcggaa gacgagcggc tcctgcacta
     4561 cctgctctcc gatgccttcg accgccgcgg cccgtgggtt accggcgtgc ggatagacgg
     4621 aaaggtctac ggcagggcaa cccgccacac cagccccagg ctggaggagt tcttcgaggc
     4681 gttccccgag gcttccaagg gaaggatact ggagccgggc tccctggagg gcgccatgac
     4741 cgtcgagctc gcccggaggg cgagggaggt ggtgagtctg gagggcaggc cccagaacgt
     4801 cgagcgggcc gagttcctaa agggcctctt cggggtcgac aacgtcgcct tccacgtcac
     4861 cgatcttgag gcggacgatc tgtcatcgta cggcgggttc gacgcggtgt tctgttgcgg
     4921 gctgctctac cacctcccca acccccgcgc cttcgtggag cgcgcggccg ccgcggcccc
     4981 gaacctctac ctcgacacgc agtacgcctg ccccggctgg gaactcgcgg agcgggacgg
     5041 tctgcggggc tgggtcagca cggaagaccc gcaagacccc cagagcgggc tctccggcac
     5101 cgccttctgg ccaaccctgg acgaactaca caggctcctc cgggagagcg gctacgagac
     5161 cgtagtgacc ctggcccgtc cccccgacaa ccgtcacggc ccaagggtgc gcatcgccgc
     5221 cagccgcccc tagccgccga gaggtcgcac ccgcgtcggg caggcagccg atggaggcgc
     5281 gcctcgagcg cggcctctac atggcccgcc cggccgacat catccttccc tgcggtgctc
     5341 gtgccgccgc cgctcgacgg gtcgggagcc cgccttcgac ggcggccgat cagcgcgcga
     5401 cggcgggcag ttccgcctcg ccggtgagca cggcgcggcg ttggcgtccg aaggcgtcgg
     5461 cggcgaggat ggcgtccagc accgtctgcg ggtaaacgtc gaagggctcg ttgtagatcg
     5521 tctccccgtc ggcgcaggct acctcggcgg ccttccggag gtcctcctcg ctcgcgtccc
     5581 ggagtccgag gtcgtccagc gccaccggga gtcccacctc caggcagaag tccaccacct
     5641 cggcgaccgc ctcgaaggga cggtcctcca gcatcagcat cgcgatgacg ccgaaggcga
     5701 ccttctcccc gtgccagtac tggtgggtct cctccaggac ggtcagcccg ttgtggatgg
     5761 aatgcgccgc ggccagcccg ccggactcga acccgaggcc cgagagcagc gtgttggcct
     5821 cgatgacctt ctcgaccgag ggggtgatcg cgccgcgctc ggcggcgagg cgggcggcga
     5881 ggccgtgctc cagcagcgtg tcgtagcaca ggcgcgcgat cgccagggcg gcctgcgtgg
     5941 gcgcgcccgc gacgagcgtc ttcgccctgg tgcgcgagga ggcgtccgcc tcgaagtacg
     6001 tactgagacc gtcgcccatg ccggccacca ggaagcgcac cggggcgccc acgatgacct
     6061 cggtgtccac gatcacggcg tcggggttgc gggcaaagag gcggtactcc agtacctcac
     6121 cctcctcgtt gtacacgacc gagaccgcgc tcgtcggggc gtcggtggag gctatggtcg
     6181 gcacgttgac cagcggcaac ccggccgggt gggcgaccgc cttggccgtg tccagagtct
     6241 tcccgccgcc gatgccgacg atcgcgtccg cgcccgcccc gcgagcctcc tcggcgaccc
     6301 ggtctatctc ccccggcgag cactcgccgc cgaactcgac gtgggtcgcc ccggagatcc
     6361 cttctagggc atcgcccatg atctcctcga cgatccggtc ccgaacgata agcggcctct
     6421 cagacttgag ctgctcgaga acctcgccca gccggcctac ggcgccccta ccctgaatgt
     6481 acctgccggg cgcggcgaac acggtggcaa gcggcccgct tgcgtctgcc tgcatgatcc
     6541 tcctcccagg tgtcggtatg tggcccaatc gtaaactcac cgcacctttg ggtcaatcga
     6601 tgccagcgat gctagcaacc ggtgggtggc tgccaccccg taccggctcg ggcgtgaatg
     6661 ggcttccctc ggtaagacgg gcactctaaa gtggggacgc cgagtccccg gaaaaggaag
     6721 tccgggtgcc gggaccaaca ccgcactacc cgccggaatt caagcgagaa gccgttcggc
     6781 tcaaccgctc ttctgagaag tcgatcccga agatggccga ggagctaggc atagccagcg
     6841 agtccttgag gagatggatc cgccaacacg aggcagatgt cggcgaacgt gaggggatca
     6901 cgaccgaaga gcgggaggag ttgagcaggc tccgccggga gaaccgcata ctcaaacagg
     6961 agaaggaggt cttgcgaaaa gcgagccaaa ggctttgcct ggccttcttc accagggagg
     7021 acgtcatccg gtgaaccttt tcaggttcat tgacgcggag agggcctacc tacccgtcgc
     7081 gctgttgtgc cagatgctag ggggtttcca ggagcggcta ctatgcctgg aaggatagac
     7141 cgccttccaa aaggagccac ggggatgccg ctctcacggc gaagatccgc gagatccata
     7201 gcaggagccg ccaaacctac ggatctccga gggtacatgc cgagttgagg acgctcggga
     7261 ccctctgctc tcgcaagcgt gtcgagaggc taatgcgcaa gagcgtactg cagggctgca
     7321 tgcgcggcag gaggagagga accacccgcc gaggcaaacg tgcgccggcc ggggacctcg
     7381 ttaagaggga cttctcggcc acaggagtgg acagggtctg ggcggcggac atcacctgcg
     7441 ttgccacgag agagggtttc ttgcacctgg ccttcatcct cgacgtccac tccagaagga
     7501 tcgtcgggtg agcgatggag agccatctca ggaccgaact tgttatcgat gccctgcaaa
     7561 tggctgtgtg gaggaggcag ccctctccgg gtctggtgca tcattccgcc cagtacactg
     7621 cgctctcgtt ctcggagagg ctcagggaag tagtaggcat caccccgtcc atgggaagga
     7681 ccgggagtgc cctggacaac gccatggcag agagcttcgt ttccaccttg aaagccgagc
     7741 tggtgagcaa ccttaagttc tccagccggc aggccgcaaa gacggccata ttcgagtacc
     7801 tggaaactgt ctataacagc cgccggctgc actcggctct tggctacagg agccccgccg
     7861 ccttcgagga agatagagtg gaaggagcca gggtcgcgta aggtaaacgt gtccgctcta
     7921 gcggggaagc ccacgtagga gaagtcggtg gtgcatgagc tacaaatctt gacacaaagt
     7981 ccagactctt agggcatagg gagtgacagt gtgacgagca atagaggtgc ccgagcagtg
     8041 ctcgcgggcg cgtactccac gagctgcgtt tctgtctgct ggccgagtgc aataatgaac
     8101 agccgccatg acgagcggtg agaagtgcta agcctcgcta gtagatggga ctttgatctt
     8161 ccatgacgca gaatgccaga cgactacgat ccatcatagc cgacaatcac cagatcggcg
     8221 gcctgctcgc gcagcggtat caacgcctga tacgccgggg aacggtacca gcctctcatg
     8281 gcgttcgcat cggggaaccg gatgacgacc gcgtcgtcat agtcgtgctc gccgctaagg
     8341 acttcgacgc gcttgccgcg gaagactatt tcggcgcccc actcggatag ggtggcgggc
     8401 acattggcgc gatactcggc ccacttttct tcgtccttga ctctgatgtg gccgatcgcg
     8461 taagctgcat tctcttccat attgcctcct gtatcggtaa ggtttagagg cgcaggatct
     8521 ttcgcgcctc ggccggtgtg gccacgggac ggccgagtgt ccgggacatg tcggcgacca
     8581 tctttaccag gtcgctgtta cgcggcgtcc cgaaacgtga gtagggatag tcgccaagcc
     8641 cgatgctcac gtggccgccc agcgtgatcg cccaggcggc cagcgccata gcgtcaccgt
     8701 ttcggcagtg cacggtccag gggtctccct ccgggatcgc ctccaggaaa gtctgaaggt
     8761 tggcgggcgt tggcggagga ccccccggca cctcatctcc cgtgaacccg agacaccaga
     8821 gggtcgccgg gttgagaagc cccatcttct gcaggttgcg cgccgtgcga accgcgcccg
     8881 cgccccagac gaccgtatag acgtacaggt cgttctcctt caggacgccc agcagctcct
     8941 tgaggtatgc gaccctgttg acgatgacct ggtcgtccgt caggaagcgt tcggcttcct
     9001 ggtcccagat gtccgcgccg aaggcgccga agtccaccgg caccatgtcg atccgcgtct
     9061 tcgggtcctc gttgagttcc agtatgtggc ggacgcgtcc ctgagcgtcg ccctgggtgg
     9121 cgataaagcc gagggtcggg tgcaatagca ggtcgtccgt ctgctcccgg accccctcga
     9181 tcacctcgcg gtacagcgtt acgtcgttcg ccctctcgct accgtccgta tcccgggcgt
     9241 gccagtgcag tatcgaagcg cccgcttccc tggcgcgcag accttcctcg accacctccc
     9301 gcggaccgta aggcagagcc cggttgtcgc cccggtccgc cgactcgttt actcgaacct
     9361 ctatgataag aggggtctgg tcgtccacag cctacctcct tgtacgggtc ctcagaccga
     9421 tccgcccgac gtgatcttgg tcacgctctc cttgatgttt tccggctcga acaccttctt
     9481 ccagtctggc tggaagatgt ctttcttggc ctcttcgacg gccatctgcg ccccggcgga
     9541 gagcgaccag tcgagctcgt cgaagaggat ggccagctcg acgttgatgt gccccagcac
     9601 gaagtctcgg gccgcctcga acgggacccc ccgttcggcg gcctcatcca ccgcctcccg
     9661 tatgacggtc aggcaggtcg ccgtcaccgt ctccgaaagc acgggttcga ggatcgccat
     9721 ctgctcgacg gtgacacggt gggagcgcag tacgggtccc cacagcttgg tggcgatctt
     9781 ctcgcccctg gcataatctt cctccggccc ctgcatgagg gcgttgacga acgcctgctt
     9841 ggccttcccg cttccgaagt aatcccgccg cgcttcggga tcggtctcgt cgttgtagat
     9901 cggcgggtgg gcggggtggg ttatgaagta ggagatgtct tccctctccg gcaactctcc
     9961 ggcgtaggcc gcggccgggt ccagggtgac gaccatggtc ccgctcttca gggacggcac
    10021 gatctccctc gccagtgagc ctacgacggt atcgggcacc gcgaagatga ccacgtccgc
    10081 ctccccgacg gcctcttcct gggaggtggg atcgaatccc ttctcgcgca accgggcttg
    10141 tccggcttca cccgcctcca cgtagagcat cgtgtactcg gggtcgtcct tgagcgcgtt
    10201 ggttacacgg acacccatac tgccggccgc tccgaaaac
//
LOCUS       DASSMB010000117         6721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_405955, whole genome shotgun sequence.
ACCESSION   DASSMB010000117 DASSMB010000000
VERSION     DASSMB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405955"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..850)
                     /locus_tag="VFJ72_07285"
     CDS             complement(74..850)
                     /locus_tag="VFJ72_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7479303.1"
                     /translation="MDEPRTEDQNLIEDNSTPSWFRLKAALVHLYTASGAVLALLILV
                     AAFQGEAVRALWLMLAALAIDSTDGLLARKMRVSEALPFFDGAMLDNIVDYMTYVFAP
                     MALLWSEGYLPEGNAGLIFAALPLLASSYQFCRVDAKTDDHYFLGFPSYFNVVAFYII
                     VFDVSAVGAAWLLAICSILVFIPIRYVYPTRTVAFRRLSLFLTTLWLISYAVILFQMP
                     EPKPLILGFSLLYLLYYFGLSLYLSGKLLEARRLEQEAME"
     gene            complement(1095..2357)
                     /locus_tag="VFJ72_07290"
     CDS             complement(1095..2357)
                     /locus_tag="VFJ72_07290"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7479304.1"
                     /translation="MLTLGGSRGDVQPYVALGLGLKAAGHEVRLAAPGTFEGLIRERG
                     LDFHRISLDPQEAVRRQLSKGDANLLEFAWRSRSILGPILQENTQQFLEACRDAEAII
                     YTSIGFLGYGIARDLGLPRVGAMYGPLINSSRYFPSSFVPVPSGRLDAAAESLPRRLY
                     NRLSYTASQQLIWQLLRAPVNRALAATPGLSPYPLDGPFGEVRRLREPALNGWSPHVL
                     PQPPDWGSHLPVTGYWFLDRPEGWRPPGGLADFLDSGPPPVSVGFGSMSGAGAEELTE
                     AVLRALKSTGSRGVLVTGWGGIANADLPDEVFKVSEVPHDWLFPRVTAAAHHGGAGTT
                     AASLRAGVPTIVVPFFADQSFWGARVAGLGVGPRPIPRARLTAERLAAAIHQAGDAGM
                     RRRARELGEKIRAEDGVARAVEAFHRFA"
     gene            complement(2398..2607)
                     /locus_tag="VFJ72_07295"
     CDS             complement(2398..2607)
                     /locus_tag="VFJ72_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479305.1"
                     /translation="MEETKAKILKVLTAIPKGVLYSTTDWHRILGDDKREIRHALDAL
                     ESEGKIEVVRSEEGRPDKPLYRLKQ"
     gene            complement(2607..3599)
                     /gene="trpS"
                     /locus_tag="VFJ72_07300"
     CDS             complement(2607..3599)
                     /gene="trpS"
                     /locus_tag="VFJ72_07300"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET7479306.1"
                     /translation="MEKRQRVFSGIQPSGTSHLGTYLGALKNWVALQDDYENYFCLVD
                     LHALTLPQDPKALRSNIRELAAIFLGVGLDPARSVIFRQSHVSEHAELCWLLNCVAKV
                     GELSRMTQFKDKAQKGGAEAASVGLYDYPVLQAADILLYNAHRVPVGEDQRQHLELSR
                     VLARRFNGTYGETFVVPEPMILKTGARIMALDDPKKKMSKSSPAPSSYIALLDDPDTV
                     RKKIRRAKTDSGSEVTASPDKPAITNLLDIYSAMTGKPVEEIEADYQGKGYGDFKKDL
                     GEVVVEGLSPIRERALELLDDPKELDSILALGAEKARGVAHATLHEAKAKMGLD"
     gene            3709..5349
                     /locus_tag="VFJ72_07305"
     CDS             3709..5349
                     /locus_tag="VFJ72_07305"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET7479307.1"
                     /translation="MPELVLGPLLRHAGSTDATIWVETDAACEVEVLGCKSRTFRVGE
                     HHYALVHVTGLEPGGSHEYEVRLDGEKVWPEEGSGFPPPVIRPMSGGEAVKIVFGSCR
                     IAAPHEPPYTLSSDEDERGLGVDALYAMAMRLRDKPSDELPHALVLLGDQIYAHKPPF
                     DTLDFIRSRRSTEQAPGEGVADFEEYARIYCDSWGDPAIRWLLSTVPSAMIFDDHEVG
                     DDWNISEAWVEEMRQEPWWNDQIVGGYASYWIYQHIGNLPPGELERDDLYNEVRNADD
                     AWPILQDFAYRTHRGPNGTRWSYHRDFGNSRLLMIDSRGGRILEEERRSMVDAEEWAW
                     IQATATGGFDHLLLGTSIPLLLGPGMHYLQSWNERLCYGAWGVRAARWSEGLRRSQDL
                     DHWSSFHDSFAAMVDLIQSIANGRRGAPPSTVLVLSGDVHHGYLAEAAFDGGTESRVY
                     QAVASPLRNSLPGKKSRLQANAWKKPAALAGRLLARLAGVEEEPLTWSLTHEAAFFDN
                     QVATLSLDGRHASITFEKAVLAGPDEPGLEKLYSCRLA"
     gene            complement(5397..5792)
                     /locus_tag="VFJ72_07310"
     CDS             complement(5397..5792)
                     /locus_tag="VFJ72_07310"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HET7479308.1"
                     /translation="MTRTFSLVIGIVFLVVGILGFIPGLTTGGGEYLLGIFAVDAMHN
                     VIHLLVGALGIAAYYWYQAKLYCQVLGVVYLLVGILGFIPAFIFGEKLLGIFAVNLAD
                     NLLHLVVGAAAAYLGFAPEVSGRRISPTH"
     gene            complement(6154..>6721)
                     /locus_tag="VFJ72_07315"
     CDS             complement(6154..>6721)
                     /locus_tag="VFJ72_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7479309.1"
                     /translation="YRLLSALEAHRLVAREDGRYALGLRLIGWGSKAVGRDLIESSRP
                     VLAALRDATGESTQLYVREGDNRVCVAAVERTGGGLRDAVPVGAVLPLGRGSGGRVLL
                     AWAGDGERFHTARDLEETRERGWTESVAERETGVASVSAPVFGPDGKARAAISASGPI
                     SRLGDHPGERLAEHVVAAAREIEQSLAP"
BASE COUNT         1205 a         2259 c         2120 g         1137 t
ORIGIN      
        1 tcgcggatcg tccggtgagc ctagcaacgg cgtgcgggcg cgtcaaagag tggcgccggg
       61 cggagcaggt ttgttactcc atcgcctctt gttcgaggcg gcgggcttcg aggagcttgc
      121 cggagaggta gaggctcagg ccgaagtagt agagcagata caggagtgag aagcccagga
      181 tcaggggctt cggctccggc atctggaaga gtatgacggc gtagcttatg agccagagcg
      241 tggtgaggaa cagcgagagc ctacggaagg cgaccgtgcg cgtcgggtag acgtaccgga
      301 tcgggatgaa gaccaggatg gagcagatgg cgagcagcca cgccgcacct acagcgctca
      361 cgtcgaagac tattatgtag aaagcgacga cgttgaagta gctgggaaag ccgagaaagt
      421 agtggtcgtc ggtcttggcg tccacccggc agaactggta gctcgaagcc aggagcggca
      481 gcgcagcgaa gatcaaacca gcgttgcctt ccggcaggta tccctcgctc cacagcagcg
      541 ccatcggcgc gaagacgtag gtcatgtagt ccactatgtt atccagcatg gcgccgtcga
      601 agaacgggag cgcctcgctc acccgcatct tgcgggccag gagaccgtcc gtgctgtcta
      661 tggcgagagc ggccaacatt agccagagag cccgcacggc ctcgccctgg aaagcggcca
      721 ccaggatcaa gagcgccagc accgcgccgc tcgccgtgta aagatgcacc agcgcagcct
      781 tgagccggaa ccacgacggc gtgctgttgt cctctattaa attctggtct tcggtgcggg
      841 gctcgtccat ccggctttta tacccttgtc ctggtatccc atacgctcgc gcttatgata
      901 acagtattga tcctcataca agtaagtttt cttctttcgt tttcttccgc agagcagcgt
      961 cctgtccggt cctcagaccc cggatgcggt ttatggcggc gcagctcgtg agggtatctt
     1021 cagaaagcgc cgggaccccg ctacgctacc atagcgctcc cacgctgatc gaacccttca
     1081 tcgtgccgcg gactctacgc gaagcggtgg aacgcctcga cggcgcgggc cacgccgtct
     1141 tcggccctta tcttctctcc cagctcccgc gcccgccttc gcatcccggc atccccggcc
     1201 tgatggatcg ccgccgcgag acgctccgct gtgagcctgg cgcgcggtat gggccgcggc
     1261 cccaccccca gccccgccac ccgcgctccc cagaaggact ggtcagcgaa gaacggcacg
     1321 actatggtgg gcaccccggc ccgcagcgag gcggcggtcg tccccgcgcc accgtgatgg
     1381 gcagcggcgg tcacgcgcgg aaagagccaa tcgtgaggga cctcgctaac cttgaacacc
     1441 tcatccggca ggtcggcgtt cgcgatgccg ccccagcccg taacgaggac gccccggctg
     1501 cccgtactct tgagggcgcg aagcaccgct tcggtcaact cctcggcccc ggcgccgctc
     1561 atgctgccga agccgacgct taccggtggc ggtccggagt ccaggaagtc cgccagcccg
     1621 ccgggcggac gccagccttc gggacggtcc aggaaccagt atccggtcac gggcaggtga
     1681 gatccccagt ccggtggctg aggcaggacg tgcgggctcc aaccgtttag cgcgggctcc
     1741 cgcaggcggc gcacctcccc gaagggtccg tcgagagggt agggagagag gcccggtgtg
     1801 gctgcgaggg cgcggttcac cggcgcgcgc aggagctgcc agatgagctg ctgggacgcg
     1861 gtgtagctga ggcggttgta gagcctgcgt ggaagactct cagcggcggc gtccaggcgt
     1921 ccggagggga cgggtacgaa ggagcttggg aagtagcgcg aggagtttat gagcggaccg
     1981 tacattgcgc ccactcgcgg aagccccagg tcgcgggcga tgccgtatcc gaggaagcct
     2041 atggaagtgt agatgatagc ctcggcgtcc cggcaggctt ccaggaactg ttgcgtgttc
     2101 tcctgcagga tgggacccag gatagagcgc gagcgccagg cgaactccag caggttcgcg
     2161 tcgcccttcg agagttgccg gcggacggcc tcctgcggat cgagcgagat gcggtggaag
     2221 tccaggccgc gttcccggat cagaccctcg aaagttcccg gcgcggcgag acggacctcg
     2281 tgcccggcgg ccttcaggcc cagtccgagg gccacatagg gttgcacgtc gccgcgcgag
     2341 ccgccgaggg tcagcatgat cctcaccgtc cggactcctc cgactcaggt gttccggcta
     2401 ttgcttcagc ctgtagagcg gcttgtccgg gcggccttcc tcgctcctta cgacctctat
     2461 cttgccctcc gattcgagcg cgtcgagggc gtgccggatc tcgcgcttgt cgtcgccgag
     2521 tatcctgtgc cagtcggtgg ttgagtagag gacgcccttg gggatggcgg tgaggacctt
     2581 gaggatcttc gctttcgtct cctccactag tcgaggccca tcttcgcctt ggcttcgtgc
     2641 agggtagcgt gggcgacacc tcgggccttc tccgcgccca acgccaggat actatccagc
     2701 tccttcgggt cgtccagcaa ctccagcgcc cgctcgcgga tgggggagag cccctcgacg
     2761 acgacctcgc cgaggtcctt cttgaagtct ccgtagccct tgccctggta atccgcttct
     2821 atctcttcga cgggcttgcc ggtcatggcg gagtagatgt cgaggaggtt ggtgatggcg
     2881 ggcttgtcgg gggaggctgt gacctccgat ccggagtcgg tcttggcgcg acggatcttc
     2941 ttccgcacgg tgtccgggtc gtcgaggagc gcgatgtagc tggagggcgc gggcgagctc
     3001 ttgctcatct tcttcttcgg gtcgtcgagc gccatgatgc gcgccccggt cttgaggatc
     3061 atgggctccg gcaccacgaa ggtctccccg tatgtgccgt tgaaacgccg cgccaggacg
     3121 cgggatagct ccaggtgctg gcgctggtcc tcgccgacgg ggacgcggtg cgcgttgtag
     3181 agcaggatgt cggcggcctg aagcaccgga tagtcgtaga gcccgacgct cgcggcctcc
     3241 gcgccgccct tctgggcctt gtccttgaac tgcgtcatcc gcgacagctc cccgaccttc
     3301 gccacgcagt tcaggagcca gcagagttcg gcgtgctccg agacgtggct ctgacggaag
     3361 atcacggagc gcgcggggtc cagcccgacg cccaggaaga tcgccgccag ctcccttata
     3421 ttcgaccgca acgccttcgg atcctgcggc agcgtgagcg cgtgcaggtc aacgaggcag
     3481 aagtagttct cgtagtcgtc ctgcaacgcc acccagttct tcaaggcccc gagatacgtc
     3541 ccgagatgcg aggtcccgct cggctgtata ccgctgaata ctctctgtct cttctccatg
     3601 ccgcgtattc tagcggaagc ggcttcaatg acgagccgct atgcgtcgcc tgatgtatcc
     3661 ttccgagatc cacgacgaga gagggccgaa tgcgcgagga tgcaccgaat gcctgagctg
     3721 gtcctcggcc cgctcctgcg ccacgccggg tccacggacg ccacgatctg ggtcgagacg
     3781 gacgccgcgt gcgaagtaga ggtgctcggc tgtaagtcgc gcacgttccg ggtgggggag
     3841 catcattacg ccctcgtcca cgtcacgggg ctggagcccg gcggctccca cgagtacgag
     3901 gtccggctgg atggcgagaa agtgtggccg gaggagggtt cggggttccc gccgcccgtg
     3961 atccgaccga tgagcggcgg cgaggcagtg aagatcgtct tcggctcgtg ccgcatcgcc
     4021 gccccccacg agccgcccta caccctgagc agcgacgagg acgagcgggg cctcggggtg
     4081 gatgcgctgt acgcgatggc gatgaggcta cgggataagc cgtccgacga gttgccgcac
     4141 gcgctggtgc ttctggggga tcagatctac gcccacaaac cgcccttcga cacgctggac
     4201 ttcatccgct cccgccgcag cacggagcag gcgcccggcg aaggcgtcgc ggacttcgag
     4261 gagtacgcgc gtatctactg tgactcatgg ggcgacccgg cgatccggtg gttactctcc
     4321 accgtcccgt ccgccatgat cttcgacgac cacgaggtcg gcgacgactg gaacatctcc
     4381 gaagcctggg ttgaggagat gcgccaggag ccctggtgga acgaccagat agtcggcggc
     4441 tacgcttcgt actggatcta ccagcacatc ggcaacctac cgcccgggga gctggagcgc
     4501 gacgacctct acaacgaggt gcgaaacgcc gacgacgcct ggcccatcct ccaggacttc
     4561 gcctaccgta cccaccgcgg acccaacggg acccgctgga gctaccaccg ggacttcggc
     4621 aactcccgtc tcctcatgat cgactcccgc ggcggacgaa tcctcgaaga agagcgccgc
     4681 tcgatggtag acgcggagga gtgggcctgg atccaggcca cagccaccgg cggcttcgac
     4741 cacctcctgc tcgggacctc catcccgctg ctgctcggcc ccggtatgca ctacctccag
     4801 agctggaacg agagactctg ctacggcgcc tggggagtgc gagcagcccg gtggtccgag
     4861 ggactgcgcc gttcccagga cctcgaccac tggtcctcct tccacgactc cttcgccgcg
     4921 atggtggact tgatccaatc catcgccaac ggcaggagag gcgcgccacc ctccaccgtc
     4981 ctcgtactct ccggcgatgt ccaccacggc tacctggccg aggccgcgtt cgacggcgga
     5041 acagagagcc gcgtctacca ggcggtagcc tcgcccctgc gaaactcgct ccccggaaag
     5101 aagtcccgac tgcaagccaa cgcctggaag aagcccgccg cacttgccgg acgcctgctc
     5161 gcccggcttg ccggcgtcga agaggagcct ctaacctgga gcctaaccca tgaggccgcc
     5221 ttcttcgaca accaggtagc caccctctct cttgacggcc gtcacgcctc cataaccttc
     5281 gagaaggccg tcctcgccgg gccggacgag ccgggcctgg agaagctcta ctcctgccga
     5341 ctcgcgtaga agagccgggg ctttcacacc ccggctaaag tcctgccgat aaactctcag
     5401 tgggtgggcg agatgcggcg tccgctgact tcgggggcga atcccaggta ggctgcggcc
     5461 gcgccgacga ccaggtgcag caggttgtcc gccaggttta cggcgaagat accgagcaac
     5521 ttctcgccga atatgaaggc ggggatgaac ccgaggatac ccacaagcag atagacgaca
     5581 cccagcacct ggcagtacag cttcgcctga taccagtagt aggcggcgat gcccagagcg
     5641 ccgacgagca ggtggatcac gttgtgcatg gcgtccacgg cgaagatgcc cagcaaatac
     5701 tcgccgccac cagtcgtcag gccggggatg aacccgagga tacctaccac cagaaacacg
     5761 ataccgatca cgagagagaa ggtgcgagtc atgggacctc ctttagagac tcgaccgtat
     5821 acgcacgaag ccctgaacat ctaactaccc gaccccgcta gacacctcct ccgcagagag
     5881 agcatccctt ggaagacata aaaggacgga ccgggatacg cgcatcagcc cgccgcgctc
     5941 cggcatgagc cgaataaaga aacattccgg ctttcacccg acacactcga agacgttcct
     6001 gaaaaaccct tgcacgcttc acgatccgca cctctacccg tctccccaag atatatcttc
     6061 ttccagtaac gttggtgaac cgcgcgttaa gaacttcgtc gcaaaagtta tttatttgtg
     6121 gaggttgggt cccgaccttc ttgaagcaat aggtcaggga gccagagact gctctatctc
     6181 ccgcgccgcc gccacaacgt gctccgccag tcgctctccg ggatgatctc ctaagcgcga
     6241 gatcggaccg ctcgcgctga tggccgcccg cgcctttccg tccggcccga agaccggcgc
     6301 gctgacgctc gcgacccccg tctcccgctc cgcaacgctc tcggtccagc cccgctctct
     6361 ggtctcttcg aggtcccgcg ccgtgtggaa cctttccccg tccccggccc acgcgaggag
     6421 caccctgcct ccagaaccac gaccgagcgg caacaccgcg cccacgggca ccgcgtcccg
     6481 caagccgccg cccgtccgct cgacggcggc gacgcacacg cgattgtcgc cttcccgcac
     6541 gtagagctgc gtgctctctc cggtcgcatc cctcagggcg gccaacacgg gccgggacga
     6601 ctctatgagg tcccgcccca cggccttgct gccccagccg atcaaacgca gacccagcgc
     6661 gtaccgccca tcctcccgcg ccacgagccg atgcgcctcc agcgccgaca acaacctgta
     6721 c
//
LOCUS       DASSMB010000118         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_658915, whole genome shotgun sequence.
ACCESSION   DASSMB010000118 DASSMB010000000
VERSION     DASSMB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658915"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFJ72_07320"
     CDS             complement(<1..437)
                     /locus_tag="VFJ72_07320"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7479310.1"
                     /translation="MESHITFEGRDRDLDGYMALPEGGERLPGVVVIQEIWGLEEHIE
                     DVTRRFAAEGYAAIAPDLYTGEWREVMSAERVAAGMAFLRSASQEVQRDPEKLESALA
                     DKPQEERDAVKMLATAMSPQGRLRFAIDTLEAVEYLKGRPEVDA"
     gene            complement(454..873)
                     /locus_tag="VFJ72_07325"
     CDS             complement(454..873)
                     /locus_tag="VFJ72_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7479311.1"
                     /translation="MSATKELRRVGEVAERLGVSPRTIKYYEEIGLVEPARSQGGFRL
                     YGEREVERLERILRMKGMGYSLAAIREILAVRDTAQEADKVTVLKTVRRHLKEQEREA
                     EGRIRQMREDLKRAETLREDLRRDIALCERRLQELGG"
     gene            1027..2505
                     /locus_tag="VFJ72_07330"
     CDS             1027..2505
                     /locus_tag="VFJ72_07330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479312.1"
                     /translation="MQEAVRERKRGSRLHLEYKWSALSCTSLGALIATINGGTLIIAL
                     PELAKELHAPLFSLVWVILGYMLAQTALVLMVGRFADMIGRKKLYVGGFALFTLVSLI
                     AGFSTTVGQLIVARVFMGAAGAFMMANSGVIVTDAFPRKELGQALGVNQMVAAVGSIL
                     GPVVGGWLTTYSWQWVFWFNVPLGIIGTLWAAWNLRELVDVERGQRLDIPGNVSFLVG
                     FTALLIGLTQGGIKGWGSDIVVASLATAAVLIPAFILIELKSKEPLLNLRMFRSRVFA
                     FGNISALLNALARMAVTFLFVFYFIGARGYDHLTTGFLLTPLAGAMFVFAPLAGRLAD
                     RLPARVLSSAGMLITAAGLVGMTTIGVHTSYWEIALWMVVVGAGSGIFNSPNTRAIMG
                     SVGPEKRGVASGTRTLVVNVGAVLSIAFAIAMVTSAMPVSAMVRIFSGVSHGLSASAA
                     APFISGLHAALLVMAGASIVGFFFSALRGSERDARAMRESGI"
     gene            2572..3750
                     /locus_tag="VFJ72_07335"
     CDS             2572..3750
                     /locus_tag="VFJ72_07335"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479313.1"
                     /translation="MGLEERPENAGSVWRQPKAVWAVAFACVVAFMGLGLVDPILPAI
                     ARQLRATQSQVELVFTSYFLVTGLAMVITGAVSSRLGHKRTLLIGLALIVLFSALAGA
                     SGGISGIVGFRAGWGLGNALFIATALAVIVEVSNAGTEQAVILYEAALGLGISVGPLL
                     GGLLGGISWRGPFYGVAVLMTIGFIAISVLLPSIPTHERKSSVLDPIRALGHRGLLTL
                     GLTALCYNFGFFTMLAYTPFPLRLDAHGLGMVFFGWGVMLAITSVFFAPRLQARFGTL
                     PVMYTMLFLIALDLAVMGLGISSRIVLILAVIIGGAFLGVNNTLITTAVMQASPVERP
                     VASAAYSFIRFVGGAAAPFLAGILAEQFTPGIPFYVGAIACVISIFVLMSGRAYLKMV
                     "
     gene            complement(3770..5311)
                     /locus_tag="VFJ72_07340"
     CDS             complement(3770..5311)
                     /locus_tag="VFJ72_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HET7479314.1"
                     /translation="MSNGKLDPVTLSVLASALAGVAEEMGAVLIRGAYSSNIKERRDC
                     STALFDAQGRMVAQAEHIPVHLGAMPEAVKAIMERDAEPGDVFAINDPYSGGTHLPDI
                     TLVSPVALMGEVIGYAVTRAHHSDVGGMRPGSMPSDSREIYQEGIIIPPVRLVRGGEY
                     VEDVLNLLLANVRTPDLRRGDLRAQIAANRIAEERVGELIERRGKETILTAFDEVISY
                     TEKRTREAIRELPDGEYFAEDFLEGDGTTDEDIKIQATVTIDGDSMTIDFSGTADTVR
                     GNVNCPLPVTRSSCYFALRVLLPKDVPANAGTYAPLEIKAPEDSLVNAAYPAAVVAGN
                     VETSNRIADTVLAALSGFAPEEIPAQGQGTMNNTIIGGRGWTYYETIGGGQGASAKGP
                     GPSGVHVGMSNTLNTPIEAFELEYPMRVERYELRYGSGGEGKHRGGDGIVRSVKVLQP
                     ASLSLLTDRRRHPPRGIEGGEPGEVGRNLLNGDELPAKVSRELESGDVVTVETPGGGG
                     YGSSG"
     gene            complement(5304..>5560)
                     /locus_tag="VFJ72_07345"
     CDS             complement(5304..>5560)
                     /locus_tag="VFJ72_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET7479315.1"
                     /translation="TVPVEKPDLDEPEPRGVAETERREANFDGEWREVPVLDRETLGR
                     GSEVEGPAIVEFREATCVVRPGWRGTVDEVGTLVLEKGDE"
BASE COUNT          806 a         1949 c         1702 g         1103 t
ORIGIN      
        1 gcgtcaacct ccggccgccc cttcaggtac tcgaccgcct caagcgtgtc tatcgcgaac
       61 cggagacggc cttgcgggct catggcggtg gcgagcatct tgacggcgtc ccgctcctcc
      121 tgcggtttgt ccgccagggc gctctccagc ttctccgggt ctcgctgcac ctcctgcgag
      181 gcgctcctga ggaacgccat ccccgccgcg acgcgctccg cggacattac ctcccgccac
      241 tccccggtgt acaggtccgg ggcgatagcc gcgtatcctt ccgcggcgaa acgccgcgtc
      301 acgtcctcta tgtgctcctc aagtccccat atctcctgga tgaccacgac gccgggtaac
      361 ctttctccgc cttccggcag cgccatgtag ccgtccaaat cccggtcccg gccctcgaac
      421 gttatatgcg actccatctc aggctcctcc cgttcaaccg ccaagctctt gcagccgccg
      481 ctcgcacagc gcgatatccc ggcgcaggtc ctcgcgcagc gtctcggccc gtttgaggtc
      541 ttcgcgcatc tgacggatgc gtccttccgc ctcccgctcc tgctccttga ggtggcggcg
      601 caccgtcttc aggacggtga ctttatccgc ctcctgcgcc gtgtctctca cggccagtat
      661 ctcccggatc gccgccagcg agtagcccat ccctttcatc ctcaggatgc gctccaggcg
      721 ctccacctcc cgctccccgt agagccggaa gcccccctgc gagcgcgcgg gctcgaccag
      781 gccgatctcc tcgtagtact tgatcgtccg cgggctcacc cccagccgct ccgccacctc
      841 cccgacccgt ctcaactctt ttgtggcgct catgaatatc cctcattccc gaagctcgta
      901 ataaccctcc catcaatata ttttcgcacg gtgaatcgtc tcactcatca aactgtacgt
      961 agacgtacag gtatattatt atatccggat ctacgaagcg ctatttgaga aatcgggagg
     1021 gtcctattgc aagaggccgt cagggagcgg aagagaggtt cgcggctgca tctggagtac
     1081 aagtggagcg cgctgtcgtg taccagcctg ggagctttga tcgccacgat aaacggcggc
     1141 acgctcatca tcgcgctgcc ggagcttgcg aaggagctgc acgcgccgct cttcagcctg
     1201 gtgtgggtga tcctgggtta catgctcgcg cagacggcgc tggtattgat ggtcgggcgg
     1261 ttcgcggaca tgatcgggcg taaaaagctc tacgtcggag gtttcgccct gttcacgctc
     1321 gtcagcctga tcgccggttt ctcgacgacc gtgggacaac tcattgtggc ccgggtcttc
     1381 atgggggcgg cgggcgcctt catgatggcg aacagcggcg tcatcgtcac cgacgctttt
     1441 ccgcgtaagg aactgggcca ggcgctcggc gtcaaccaga tggtcgccgc cgtaggctca
     1501 atactaggcc cggtagttgg cggctggctc acgacctact cctggcagtg ggtcttctgg
     1561 ttcaacgtgc cgcttggcat catcgggact ctctgggcgg cctggaacct gcgcgagctg
     1621 gtggacgtcg agaggggaca gaggctggac atccccggca acgtctcctt cctcgtcggg
     1681 ttcacggcgc tcctgatcgg gctcactcag ggcggcataa agggttgggg ctccgatatc
     1741 gtcgtggcga gcctcgctac tgccgccgtg ctgatccccg ccttcatcct catcgaactg
     1801 aagagcaagg agccgctcct caacctgagg atgttccgca gcagggtctt cgccttcggc
     1861 aacatcagcg ccctcctgaa cgcgctcgct cggatggccg tcaccttcct cttcgtcttc
     1921 tacttcattg gggcgcgggg ctacgaccac ctgacgaccg gcttcctttt gacgccactc
     1981 gcgggcgcga tgttcgtctt cgcgccgctc gccggacggc tggccgacag gctccccgcc
     2041 cgcgtcctca gttccgcggg tatgctcatc accgccgccg gtctggtcgg gatgactacc
     2101 attggcgtcc atacgtctta ctgggagata gccctctgga tggtcgtggt cggggcgggt
     2161 tccggcatct tcaactcgcc gaacacgcgg gccataatgg gctcggtcgg cccggagaag
     2221 cggggcgtcg ccagcggcac gcgcaccctc gtcgtcaacg tcggggcggt gctctccata
     2281 gccttcgcca tagcgatggt gacgagcgcc atgcccgtga gcgcgatggt caggatcttc
     2341 tccggcgtct cccacggcct ctccgcgtcc gctgccgcac ccttcatctc cggcctgcac
     2401 gccgctctgc tcgtgatggc gggcgcgagc atcgtcgggt tcttcttctc cgccctgcgg
     2461 ggcagcgagc gcgacgcccg ggcaatgcgg gagagcggca tctagccccg ttaaccaatc
     2521 gaaaaaggag cttaatccaa gttggagtct acgtcgggaa ctgatagaag catgggcctg
     2581 gaagagaggc cggagaacgc ggggagcgtc tggcggcagc cgaaggccgt ctgggcggtg
     2641 gctttcgcct gcgtggtggc gttcatgggt ctgggtctgg tggacccgat cctgccggcc
     2701 atcgcccgcc agctccgcgc cacccagagc caggttgaac tggtgttcac gagctacttc
     2761 ctggttacgg ggcttgcgat ggtcattacc ggcgccgtct ccagccggct ggggcacaag
     2821 cgcaccctgc tcatcgggct cgcgctcatc gtcctcttca gcgcgctggc cggggcctcc
     2881 ggtgggatct ccggcatcgt cggcttccgg gccggatggg gtctcggtaa cgctctgttc
     2941 atagcgaccg cactcgccgt gatcgtcgag gtctccaacg ccgggacgga gcaggcggtt
     3001 atcctgtacg aggcggcgct ggggctcggc atctccgtcg ggccgttgct cggcggtctg
     3061 ctcggcggga taagctggcg cggaccgttc tacggcgtcg ccgtgctcat gactataggc
     3121 ttcatcgcca tctctgttct gctgccttcg atccccactc acgagcgcaa gagttccgta
     3181 ctcgatccga tccgggcgct cggtcatcgc gggctcctca ccctcgggct gaccgccctc
     3241 tgctacaact tcggcttctt caccatgctg gcctacaccc ccttcccgct gcggctcgac
     3301 gcccacggcc tcgggatggt cttcttcgga tggggagtca tgctcgccat cacatcggtg
     3361 ttttttgcgc cgaggctcca ggcccgcttc gggacgcttc cggtgatgta cacgatgctc
     3421 ttcctgatag ctctggacct ggccgtgatg gggctcggga tctcctcccg catcgtcctc
     3481 atactggcgg tcatcatcgg cggggcgttc ctcggggtga acaacaccct cataaccacc
     3541 gccgtcatgc aggcctcgcc cgtggagcgc ccggtcgcct ccgccgccta cagcttcatc
     3601 cggttcgtgg gaggcgcggc cgcccctttc ctcgctggca ttctggcgga gcaattcacc
     3661 ccaggcatcc cgttctacgt cggggcaatt gcctgcgtga tcagcatctt cgtcctgatg
     3721 tctggacggg catatctgaa gatggtgtag cgaaggccgg gaacttctac tacccggagc
     3781 ttccgtaccc gccgccgccc ggcgtctcga ccgtcaccac gtcgccggac tccaactcac
     3841 ggctgacctt cgccggcagc tcatcgccgt tcagcaggtt cctgccgact tctccaggct
     3901 cgccgccctc gatgccgcgc ggaggatggc ggcggcgatc cgtcaggagc gagaggctcg
     3961 ccggttggag caccttcacg gagcggacga tgccgtcgcc gccccggtgc ttcccctcgc
     4021 cgccggagcc gtatcgcaac tcgtagcgtt cgacgcgcat cgggtactcc agctcgaatg
     4081 cctcgatagg ggtgttgagc gtgttgctca tcccgacatg gacgccggag ggaccggggc
     4141 ctttcgcgct cgcgccctgg ccgccgccga tggtttcgta gtacgtccat cccctgccgc
     4201 cgatgatcgt gttattcatc gtgccctggc cctgggccgg tatctcctcc ggcgcgaaac
     4261 cggagagcgc ggccagcacc gtatccgcga tgcggttgct cgtctcgacg ttcccggcga
     4321 cgaccgccgc cggatacgcg gcgttcacca ggctgtcctc cggcgctttt atctccagag
     4381 gcgcgtaggt gcccgcgttg gccggcacgt ccttcggcag cagcacgcgc agggcgaagt
     4441 agcacgaaga gcgcgtgacc ggcagcggac agttcacgtt gccgcgcacg gtgtctgcgg
     4501 tgccggagaa gtctatcgtc atggagtcgc cgtctatggt gacggtcgcc tgtattttga
     4561 tgtcctcgtc cgtggtgccg tcgccctcca ggaagtcttc ggcgaagtat tcgccgtcgg
     4621 gaagctcgcg gatggcctcg cgggttcgct tctcggtgta ggagatcacc tcgtcgaagg
     4681 cggtgaggat ggtctccttg ccgcggcgtt cgatcagctc tccgacccgc tcctcggcga
     4741 tgcggttcgc ggcaatctgg gcccgcaggt cgccccgccg caggtccgga gtgcggacgt
     4801 tggcgagcag gaggttgagc acgtcctcca cgtactcgcc gccgcggacc agccgcaccg
     4861 gcgggatgat gatgccctcc tgatagatct cccgcgagtc cgaaggcatg gagccgggac
     4921 gcatcccgcc cacgtccgaa tggtgcgccc gcgtcacggc gtagccgata acctcgccca
     4981 tgagggcgac cggcgagacg agcgttatgt ccggcaggtg cgtgccgccg gaatacgggt
     5041 cgttgatcgc gaacacgtcg cccggctccg cgtcgcgctc catgatggcc ttcaccgcct
     5101 ccggcatcgc gccgagatgt accgggatgt gctcggcctg ggcgaccatc cgcccctgcg
     5161 cgtcgaagag ggccgtcgag cagtcgcggc gctccttgat gttcgaggag taggcgcccc
     5221 ggatcaggac cgctcccatc tcctccgcta cccctgccaa ggcgctcgcc agcaccgaga
     5281 gcgttaccgg atcgagcttg ccgttactca tcgcccttct ccaagacgag cgtgccaacc
     5341 tcgtccacag taccccgcca gccgggacgc accacgcacg tcgcctcccg gaactcgact
     5401 atcgccggac cctcgacctc cgaaccccgg cccaacgtct cgcggtccag caccgggacc
     5461 tccctccatt caccatcgaa gttcgcctcc cggcgctccg tctcggcgac accccgtggc
     5521 tccggctcgt ccaggtccgg cttctccacc ggaacggtcg
//
LOCUS       DASSMB010000119        10009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1799001, whole genome shotgun sequence.
ACCESSION   DASSMB010000119 DASSMB010000000
VERSION     DASSMB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10009
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799001"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFJ72_07350"
     CDS             <1..273
                     /locus_tag="VFJ72_07350"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HET7479316.1"
                     /translation="ADVGTQYRSAVFYHSGEQKQIAEEVISSLETEGVWDNPIVTEVA
                     PFEEFYVAEDYHQEYFRNNGFQPYCQVIIAPKVAKFRKQYLEKLKA"
     gene            421..894
                     /locus_tag="VFJ72_07355"
     CDS             421..894
                     /locus_tag="VFJ72_07355"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479317.1"
                     /translation="MLVRMRRPVGTLFESVWERFGSGGEAERMAHARALISSGEYAPA
                     LNILARLLNAAESSGKMPVVVEVLAVRALLLRDLSEHEDAMSAIARALSFGEREGYVN
                     VFVREGAPMAALLYDFLRDQREGRIEDSPRVPPEYVGMLLAMLRVRAALPDEALV"
     gene            complement(926..1300)
                     /locus_tag="VFJ72_07360"
     CDS             complement(926..1300)
                     /locus_tag="VFJ72_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479318.1"
                     /translation="MAQNEMPRPPYVLLFQEAQPGAEPLMISVDDRPALPLFDSNYKA
                     EAFLESVDFGTGWKPIEVSGMGLLTVLETCRGAVGYIALNPPPVTGSGMTVRMGGLEE
                     LIEALEQSQEDDLFGLGGLSSN"
     gene            complement(1303..1623)
                     /locus_tag="VFJ72_07365"
     CDS             complement(1303..1623)
                     /locus_tag="VFJ72_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7479319.1"
                     /translation="MNTRAIEETISFSEEKMKKNAVFDSPHLFYDTYCLLPGQYQKVH
                     AHEGSDKVYYVLRGTGRFTVGEEERDLGEGNAVIARAGEPHGVRNESVEELVLLVTMA
                     PRPA"
     gene            complement(1661..2818)
                     /locus_tag="VFJ72_07370"
     CDS             complement(1661..2818)
                     /locus_tag="VFJ72_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7479320.1"
                     /translation="METSDSRNLLIIGTPQHDAAAYHLSGFLAPDPVICALAKGRKYL
                     AVSSLEYGRAAKQAPADELLSFDELEVNRLARELGSGAKALAAASAELLTKLGANVVS
                     VPPDLGVIYADELRARGLTVIPDGKLFTGLRRAKTEAEISHIEKTQSAVEAACARAVE
                     VLRGAEIEDDGTLNWRGEPLTSEVLRSEVDVELLRRGCAADGTITAGGPQAADPHERG
                     HGPLKAGESVIVDIFPMDLSTRYYSDMTRTFVKGDPNEGLQAMYDAVLESQEAALGMI
                     RAGVNGRDVHRKVAEILHGAGYKTNVHDQKPGEPLTEGFFHGTGHGVGLEIHEAPRVS
                     LADEELIPGDVISVEPGVYDPKIGGVRIEDLVVVTEDGCRNLTSFPKEFRV"
     gene            2908..3150
                     /locus_tag="VFJ72_07375"
     CDS             2908..3150
                     /locus_tag="VFJ72_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HET7479321.1"
                     /translation="MTEKRDMRARVEEALDKVRPALRADGGDARVVECDEKSGSVSIE
                     MLGACSGCPLSQLDFVYAIETLIRREVPDVREILAV"
     gene            complement(3152..3685)
                     /locus_tag="VFJ72_07380"
     CDS             complement(3152..3685)
                     /locus_tag="VFJ72_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479322.1"
                     /translation="MFRLYVALILLAEIIFLALLPFVAPGVPVWIFFAVLPLTLVGAY
                     VVHRLRNSASFRMRRLSPDEITRHTPAREPGAEKDRNTPEELSRLIVERTAEIRRTLT
                     ESPSEIRIEMCALGYRACVNDMISLTHLTNEELPNAGFLRRLVLRRARKRATDALTAT
                     REALPQSALRATHQEQQ"
     gene            3784..4362
                     /locus_tag="VFJ72_07385"
     CDS             3784..4362
                     /locus_tag="VFJ72_07385"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET7479323.1"
                     /translation="MQTTTSPEFTLDRPLASAADVLRGIFLAPRSFFLAFPENGPLRE
                     PVIFVVLVTAMTAVLRLALQLLFGSNDAASAGISVVEALAFVALSPAILAALAGAYWL
                     SVRTFVGEAGTYRTVYRMLAYAYGAMVLFWVPVAGAFAFTYFALVLMVLAIRYVYRVS
                     LMTAIITALVAYVPAATAFIFLQFAITSLAFG"
     gene            complement(4343..5149)
                     /locus_tag="VFJ72_07390"
     CDS             complement(4343..5149)
                     /locus_tag="VFJ72_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479324.1"
                     /translation="MQPPRTLDAYSSDATSLWHELGMLHARAGEEARRASQRGFALAC
                     AVGALVLLSAPVFGTAWAGPWAGALPVAAGVMTGGGSLLWRRLVFLRERDDLRRRLAA
                     LGHDAERPARDGLSAYYDPQLILLRSEYEFLHARGARGSARLFEESFGFTPEDPFESG
                     PLSVLPDTEEMRGLRERWERRISARREAGQEQPALGLREDRAYRVFPREMTVPMELAT
                     RRAYLEISRALLRKRHGRRIDNMAGEVRKRAERDLREHAALTRKVSRRPG"
     gene            5166..5753
                     /locus_tag="VFJ72_07395"
     CDS             5166..5753
                     /locus_tag="VFJ72_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479325.1"
                     /translation="MSTRFPRQADREKLARRLTNLLNPFFIFTALYVLVALAHSGPRG
                     LVYVVPELLAAGLVAGYVLLMRRRSRVSDFWISTRAERIIPAIVLLAAFAGLLITLYL
                     LAAPGPLILTTLSMGLAAASVAALTLFWKASAHTAVAGHAAVAGVLLLGAVGSVFVLV
                     LPLVVWARVSPGAHTLSQTLAGAGIGAVFALLFLT"
     gene            complement(5797..5869)
                     /locus_tag="VFJ72_07400"
     tRNA            complement(5797..5869)
                     /locus_tag="VFJ72_07400"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5833..5835),aa:Glu,seq:ctc)
     gene            complement(5929..7410)
                     /gene="gltX"
                     /locus_tag="VFJ72_07405"
     CDS             complement(5929..7410)
                     /gene="gltX"
                     /locus_tag="VFJ72_07405"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7479326.1"
                     /translation="MDNSQIRVRYAPSPTGFLHVGGVRTALFNWLFARKNNGTFVLRI
                     EDTDLERSTEESVEQLKRSLRWIGLDWDEGPEVGGPHPPYRQTERFDLYREEAKKLID
                     SGAAYYDFATAEELAELREKARAEKRPPIYTGGEYRDMAPGEARKKIEAGEPYTVRFK
                     TPREGQTVVEDLIRGPVTFENANIEDFVLMKSTDTPTYNFAAAVDDALMRISHVIRGD
                     DHLSNTPRQILIYKALGYPLPAFAHVPQVLGPDKKKLSKRHGAASVEDFAAQGYLPEA
                     LFNYLALLGAGYAADEEIFSPEELAERFRIEKVSGNPAVFDETKLRSINAVYLRRNSP
                     EELAMLAAPMLAESGVTSEEELQRDMPRLTRIMELLKERIALTTEIPDSVAYFYGNEI
                     AYDPAEFEKQFGKEFVRENFPELVERLKTLPEWTEEAIEECVRGLAAEKEKGARHLIH
                     PLRYAVTGRTVSAGLFETMLLLGRERCLLRAEKAVEELRKLPV"
     gene            7553..7819
                     /locus_tag="VFJ72_07410"
     CDS             7553..7819
                     /locus_tag="VFJ72_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479327.1"
                     /translation="MADITVTPAGPREFEVVVGGSGTEHHVTVPEGLIEKLNLPEDDL
                     ERVVRESFEFLLEREPASSIMREFSLDVIPRYFPEYEEELPKRI"
     gene            complement(7829..8113)
                     /locus_tag="VFJ72_07415"
     CDS             complement(7829..8113)
                     /locus_tag="VFJ72_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HET7479328.1"
                     /translation="MEKAEGAAGRDEIVSKFEEEGLDPHQWGNAPSYEYGWHSHGYHK
                     VLYCVSGSIVFHTREGDFSLRMGDRLDVEPGTEHAATVGPDGVQCLEAAR"
     gene            8308..8838
                     /locus_tag="VFJ72_07420"
     CDS             8308..8838
                     /locus_tag="VFJ72_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7479329.1"
                     /translation="MSTLPTRLEPVDVKATHWMARHSVLILRIALGVVFFWFGVLKFF
                     PGLSPAQTLAIKTIDVLTLGLVPGNVSLVLLATLECVIGLGLISGGFMRLVLLLLAFQ
                     MVGAVSPLFIFPGEVFTKVPYAPTLEGQYIIKNIVLISAGLVIGATVRGGGLVAHPEA
                     AERGREAEEREKQRHG"
     gene            complement(8853..9512)
                     /locus_tag="VFJ72_07425"
     CDS             complement(8853..9512)
                     /locus_tag="VFJ72_07425"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HET7479330.1"
                     /translation="MATAKETILWRIRRATRDVPENERPEDVAVERGYRKEGEASREE
                     IVARFAERAAEYKAAVRRVSESELPGAIAEALKQRGVEKLVIPPNLPGEWIPGGVETL
                     RDAARPRLTNEELDGSDGVLTGCALAIAQTGTIVLDAGAAQGRRALTLLPDYHLCVVR
                     EDQIVGLVPEAFAGLEDTVRYERRAVTFISGPSATSDIELNRVEGVHGPRTLEVLISG
                     E"
     gene            complement(9512..>10009)
                     /locus_tag="VFJ72_07430"
     CDS             complement(9512..>10009)
                     /locus_tag="VFJ72_07430"
                     /inference="COORDINATES: protein motif:HMM:NF023298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HET7479331.1"
                     /translation="SACLNVCPVYERVGGHAYNSVYPGPIGAILTPQLVGVGKPGPDS
                     LPYASSLCGACYEVCPVKINIPQVLIHLRGRVVRKKQDTGGLKSTLDPENVAMQAVAK
                     VFSDPERFEQAQKLARIGQWPLARSGSIHRLPGRLAGWTETRDLRAIPEQSFREWWKQ
                     NRGAR"
BASE COUNT         1518 a         3261 c         3343 g         1887 t
ORIGIN      
        1 gcggacgtgg ggacccagta ccgctcggcg gtcttctacc actccggcga gcagaagcag
       61 atcgcggaag aggtcatctc ttccctcgaa accgagggtg tctgggacaa ccccatcgtg
      121 acggaggtcg cgccgttcga ggagttctac gtggcggagg actaccatca ggagtacttc
      181 cgcaacaacg ggttccagcc gtactgccag gtcatcatcg ccccgaaggt cgccaagttc
      241 cgcaagcagt acctggagaa gctcaaggcg tagctctggc cgcgctacgc ctttaagacg
      301 caaaagatcc ctcatttggg ggatgtcggg agccttcgcc ggatggataa tcactacagc
      361 taagagttac ggtgaaggga tgcgagatga cattctcgtg tggtggatcg gaggtccccg
      421 atgctggtta ggatgaggag gccggtcggg acgctgttcg agtcagtgtg ggagaggttc
      481 gggagcgggg gtgaggccga gcgaatggct cacgcccgag ccctgatctc gtccggcgaa
      541 tacgcgcccg cgctgaacat cctggcgcgg ctcctgaacg cggcggaatc tagcggcaag
      601 atgcccgtcg tggtcgaggt actcgccgtc cgggcgctgc tcctgcgcga cttgagtgaa
      661 catgaggacg cgatgagcgc catagcgcgg gcgctctcgt tcggcgaacg cgagggctac
      721 gtcaatgtct tcgtgagaga aggcgcgccg atggcggcgc tgctctacga cttcctgaga
      781 gaccagagag agggacgcat cgaggactcg ccgcgcgtcc cgccggagta tgtgggtatg
      841 ctcctcgcca tgctcagggt gcgcgccgcg ctcccggacg aggccctggt ttgaaagtca
      901 tctgcctcgg ctcacgggcg aggcgtcagt tggagcttag accgccgagg ccgaagaggt
      961 cgtcctcctg gctctgctcc agggcttcta tcagctcttc gaggccgccc atccgcacgg
     1021 tcatgccgct gccggtcacg ggcggcgggt tgagggcaat gtagccgacc gcgccccggc
     1081 aggtttcgag gacggtgagc aggcccatgc cggagacctc tatgggtttc cagccggtac
     1141 cgaagtctac ggactcaagg aaagcctcgg ccttatagtt agagtcgaag agggggagag
     1201 cgggacggtc gtccacggag atcatgagcg gttccgcgcc gggttgggcc tcctggaaga
     1261 ggagcacgta gggcggccgg ggcatctcgt tctgggccat gatcaggccg ggcggggagc
     1321 catcgttacg agcaggacga gttcctctac actctcgttc cggacgccgt gcggctcacc
     1381 ggcgcgggcg atcacggcgt tgccctctcc gaggtcccgc tcctcctcgc cgacggtgaa
     1441 gcggccggtg ccccggagga cgtagtagac cttgtccgac ccctcgtgag cgtggacctt
     1501 ctggtactgg ccgggcagca ggcagtaggt atcgtagaac aggtgcgggg agtcgaagac
     1561 ggcgttcttc ttcatcttct cctcggagaa ggagatcgtc tcttcgatgg ccctggtgtt
     1621 caaaaattcc ctccttgtgg acctttacgc cagtactctt ctatacccgg aactctttcg
     1681 ggaagctcgt caggttgcgg cagccgtcct cggtgacgac cacgaggtct tcgatgcgga
     1741 cgccgcctat cttcgggtcg tagacgccgg gctccacgga gatcacgtcg ccggggataa
     1801 gctcctcgtc ggccagcgag acgcgagggg cctcgtggat ctccagcccg accccgtgcc
     1861 cggtgccgtg gaagaagcct tccgtgagcg gctcgcccgg cttctggtcg tggacgttgg
     1921 tcttgtagcc cgcgccgtgc agtatctccg cgaccttgcg gtgtacgtcc cggccgttga
     1981 cgccggcccg gatcatgccg agggcggctt cctggctctc cagcacggcg tcgtacatag
     2041 cttgcagacc ctcgttcgga tcgcccttga cgaaggtgcg ggtcatgtcc gagtagtagc
     2101 gggtcgagag gtccatgggg aagatgtcca cgatgaccga ctctccggcc ttgagggggc
     2161 cgtggccgcg ctcgtgcggg tcggcggcct gcgggcctcc ggcggttatg gtgccgtcgg
     2221 cggcgcaccc gcgccggagt agctccacgt ccacctcgga gcgcaggacc tcgctcgtca
     2281 ggggctcgcc gcgccagttc agggtcccgt catcctcgat ctccgcccct ctcagtactt
     2341 cgacggcgcg ggcgcaggcg gcttcgacgg cactctgagt cttctcgatg tgggagatct
     2401 cggcctccgt cttggcgcgg cggaggccgg tgaagagctt gccgtcgggg atgacggtca
     2461 ggccgcgggc gcgcagctcg tccgcgtaga tcacgccgag gtccggcggc acggagacga
     2521 cgttggcccc gagcttcgtc agtagctccg ccgaggcggc ggcgagggct ttggcgccgc
     2581 tccctagctc gcgggcgagg cggttgacct ccagctcgtc gaaggagagc agctcgtccg
     2641 cgggagcctg cttcgcggcg cgcccgtact ccagggagga gacggccagg tacttccggc
     2701 ctttcgccag cgcgcagatc accgggtccg gcgccaaaaa cccggagaga tggtaggccg
     2761 cggcgtcgtg ctgcggggtg ccgatgatca gtaagttccg tgaatccgaa gtctccattc
     2821 gttctcctct cacgtctcag atgccgtcgg aatcccagtc tactaccgac ggctgtgcgc
     2881 cgtggatact cctatataat cgggacgatg accgagaaga gggacatgcg ggcgcgggtg
     2941 gaggaggcgc tggacaaggt gcgtccggcg ctgcgggccg acggcgggga cgcccgcgtc
     3001 gtcgagtgcg acgagaagag cggctccgtc agcatagaga tgctcggggc gtgcagcggg
     3061 tgcccgctct cgcagttgga tttcgtctac gctatcgaga ccctgatccg gcgcgaagtc
     3121 ccggacgtgc gggagatcct ggcggtctag cctactgctg ctcctgatgg gtggcgcgca
     3181 gggcgctctg aggtagggcc tcgcgggtcg cggtgagggc gtcggtggcc ctcttgcgag
     3241 cgcgacggag gaccaggcgg cgcaggaatc cggcgttcgg gagttcttca ttcgtcaggt
     3301 gggtcagaga gatcatgtcg ttcacgcacg cccggtagcc gagggcgcac atctcaatgc
     3361 ggatctcgga cggagactcg gtgagggtcc gccggatctc ggccgtccgc tccacgatga
     3421 gccgggacag ttcttcggga gtgtttctat ctttttcggc gccgggttcg cgcgcgggcg
     3481 tatggcgcgt gatctcatct ggcgagaggc ggcgcatcct gaacgaagcg ctgttcctga
     3541 gacgatgaac cacgtaggct cccacgagcg tgagcggtag gacggcgaag aatatccaca
     3601 ctggaactcc tggcgcgacg aacggcagca gggccaggaa aataatctcg gccaacagta
     3661 tcagcgccac atacagccta aacatcccta ccgtgcctct cgttactcct ccggggggac
     3721 taagtctaag ttttggagaa agatttcgca agcgcttttt acccgcccgc tataatcttc
     3781 aacatgcaga ctaccacctc cccggagttt accctggacc gtcctctcgc gagcgcggca
     3841 gatgtcctgc gcggcatctt cctggccccg cgctctttct tcctggcctt cccggagaac
     3901 ggcccgctca gggagccggt gatattcgtc gtcctcgtga ccgccatgac ggccgtcctg
     3961 cggctcgctt tgcagctcct gtttggctcg aacgacgcgg cctccgccgg gatctcggta
     4021 gtcgaggccc tggcgttcgt cgcgctctcg cccgcgatac tcgccgcgct ggcgggggct
     4081 tactggctct ccgtccgcac cttcgtcggg gaggccggca cgtaccggac cgtctaccgg
     4141 atgctcgcct acgcctacgg cgcgatggtg ttgttctggg tcccggtcgc gggggcgttc
     4201 gcgttcacgt acttcgcgct ggtgctgatg gtactcgcga tccggtacgt ctaccgcgtc
     4261 tcgctgatga ccgcgatcat cacggcgctg gttgcctacg tgccggcggc gacggccttc
     4321 atcttcctcc agttcgccat cactagcctg gccttcggct gaccttccgc gtcagcgcgg
     4381 cgtgctcgcg taggtcccgt tcggcccgct tgcggacctc gccggccata ttgtcaatcc
     4441 ggcggccgtg gcgtttcctc aacagggcgc gcgagatctc caggtacgcc cgccgcgtcg
     4501 ccagctccat cgggacggtc atctccctcg ggaaaaccct gtaggcccgg tcctctcgca
     4561 gacccagcgc gggctgctcc tgcccggcct cgcggcgcgc ggagatcctg cgctcccacc
     4621 gctctcgcaa cccgcgcatc tcctccgtgt cgggcaaaac actcaatgga cccgactcga
     4681 acgggtcctc cggcgtgaac ccgaacgact cctcgaagag ccgcgccgat cctcgagcgc
     4741 cccgcgcgtg caggaactcg tactccgaac gcagcaggat gagctgcggg tcgtagtagg
     4801 cggagaggcc gtccctggct ggccgctccg cgtcgtgccc cagcgcggcg agcctccggc
     4861 gtagatcgtc ccgttccctc aagaacacca gccgccgcca cagtaacgag ccgccgccgg
     4921 tcatcacgcc ggccgcgacc ggcagcgccc cggcccacgg ccccgcccac gccgtcccga
     4981 agaccggcgc cgagagcagc accagcgccc cgacggcgca ggcgagggcg aaaccccgct
     5041 gggacgcccg tcgagcctcc tccccagccc gcgcgtgaag catccccagc tcgtgccaga
     5101 ggctcgtcgc gtcgctggag taggcgtcaa gtgtgcgcgg cggttgcacg tgctagattc
     5161 tacaggtgtc caccaggttc ccccgacagg ccgacagaga gaagctggcc cgccgcctga
     5221 cgaacctgct aaaccccttc tttatcttca ccgcgctcta cgtcctggtt gccctggcgc
     5281 acagcggccc ccgcgggctg gtttacgtgg tccccgaact gctcgcggcg gggctggtgg
     5341 ccgggtacgt gctgctcatg cgacggcggt cgcgcgtctc ggacttctgg atctccaccc
     5401 gcgccgaacg catcatcccc gccatcgtcc tcctcgccgc cttcgccggg ctactgatca
     5461 cgctctacct gctcgccgcc cccggacccc tcatcctcac gacgctctcg atgggcctcg
     5521 ccgcggcctc cgtcgccgcg cttaccctct tctggaaagc cagcgcccac accgccgtcg
     5581 ccggacacgc cgccgtcgcg ggcgtactgc tgctcggcgc ggtcggatcg gtgttcgttc
     5641 tcgtgttgcc actcgtagtc tgggccagag tttcacccgg cgcgcacacg ctttcgcaga
     5701 cgctcgccgg agccggtatc ggggcggtct ttgcgttgct ctttctgaca tagagaaagc
     5761 ccgccgtggc cggagccgga gcgggcttta ctggagtgac cccaccgggg ttcgaacccg
     5821 ggttttcgcc gtgagagggc gatgtcctag gccactagac gatggggcct tcgagcggtg
     5881 agagtttaaa cgatgctccg gggttcgtca aaggaaatcg ggcgggctct aaaccggcag
     5941 cttccgcagc tcctcgaccg ccttctcggc gcgtagcaaa caacgctcgc ggccgaggag
     6001 caacatggtc tcgaagaggc cggcgctcac ggtgcggccg gttacggcat agcgcagagg
     6061 atgtatgagg tgccgggcgc ccttctcttt ctcggcggcg aggccgcgaa cgcactcctc
     6121 tatcgcttct tccgtccatt ctgggagagt cttgaggcgc tcgacgagtt ccgggaagtt
     6181 ctcgcggacg aactccttgc cgaactgctt ctcgaactcg gctgggtcgt aggctatctc
     6241 gttgccgtag aagtaggcga ccgaatccgg gatctcggtg gtgagggcga tgcgctcctt
     6301 gagcaactcc attatccgcg tgagtcgcgg catgtctcgc tgtagctctt cctcgctcgt
     6361 cacgccgctc tcggcaagca tcggggcggc gagcatggca agctcttccg gactgttgcg
     6421 gcggaggtag acggcgttta tggagcggag tttagtttcg tcgaagaccg ccgggttgcc
     6481 gctgaccttc tctatgcgga agcgttccgc gagctcctcg gggctgaaga tctcctcgtc
     6541 cgccgcgtag cccgcgccga ggagcgccag gtagttgaag agcgcctccg gcaaatatcc
     6601 ctgcgccgcg aagtcctcga cgctcgccgc gccgtggcgc ttggacagct tcttcttgtc
     6661 cgggccgagg acttggggga cgtgggcgaa ggccgggagc gggtagccta gagccttgta
     6721 gatcaggatc tgtcgtggcg tgttggagag gtggtcgtcg ccccgtatga cgtggctgat
     6781 ccgcatgagg gcgtcgtcca ccgccgccgc gaagttgtag gtcggcgtgt cggttgactt
     6841 catgagcacg aagtcctcga tgtttgcgtt ctcgaacgtg acgggaccgc ggatgaggtc
     6901 ctccacgacc gtctggccct cgcgcggggt cttgaagcgg acggtgtacg gctcgccggc
     6961 ctctatcttc ttccgcgcct ctccgggggc catgtcgcgg tactcgccgc cggtgtagat
     7021 ggggggacgt ttctccgccc gcgccttctc gcgcagctcc gcaagctcct cggcggtcgc
     7081 gaagtcgtag taggcggcgc cggagtctat tagcttcttc gcctcctcgc ggtacaggtc
     7141 gaagcgctcg gtctggcggt aggggggatg tggaccgccg acctccgggc cctcgtccca
     7201 gtccaggccg atccagcgca aagagcgctt tagctgctcg accgactcct cggtcgaacg
     7261 ctccaggtcc gtgtcctcta tccgaagcac gaacgtgccg ttgttcttgc gcgcgaagag
     7321 ccagttgaag agcgccgtcc tgacgccgcc gacgtgcagg aagcccgtgg ggctcggcgc
     7381 gtaccttact cgtatctgag agttatccat gggctaaacc tagcacacgg ccggcgagcg
     7441 ggaaagccgc aatgccatgc tttctcgaat accgcgcgta atgcggccca ccgtcggttc
     7501 gggcgctata ctggcctgcc tgatgtcctg ctctgcgaaa ggcggcgaaa tcgtggcaga
     7561 tatcaccgtt acccccgctg gaccgcgcga gttcgaggtg gtagtcggcg gctccgggac
     7621 ggagcaccac gtaacggtcc ccgagggtct gatcgagaag ctgaatctac ccgaggacga
     7681 cctcgaacgg gtggttcggg agtcgttcga gttcctgctg gagcgcgagc ccgcctcctc
     7741 catcatgcgc gaattctcgc tagacgtgat cccgcgctac ttccccgagt acgaggagga
     7801 gctgccgaag cggatctagc cggggcttct agcgggccgc ctccaggcac tgcacgccgt
     7861 cggggccgac ggtcgcggca tgttcggtgc cgggttccac gtccagccgg tctcccatcc
     7921 gaagggagaa gtccccttct ctggtgtgga acacgatgct gccggagacg cagtagagta
     7981 ccttgtggta gccgtgcgag tgccatccgt actcgtagct gggcgcgtta ccccactggt
     8041 gggggtcgag gccctcctcc tcgaacttcg acacgatctc gtcccggccc gccgctcctt
     8101 ccgctttctc catccatgcg gacattcccg tctcctcctt ttccgctcgt gtccctcaga
     8161 gtatgccatc catcgccagg cgcttcttcc ggggttggcg tgaggccgtg aatggtttat
     8221 ttgcggattc tcggccccgg cggtatttat ccggtgtgct gaccgtattt atctgataac
     8281 ggacaacatc gttctggagg gatgccgatg agcacgttgc cgacgcggct ggagcccgta
     8341 gacgtcaagg ccacgcactg gatggcccgt cacagcgtct tgatactgcg catcgcgctg
     8401 ggggtggtgt tcttctggtt cggggtcctg aagttctttc cgggtttgag cccggctcag
     8461 acgctggcga tcaagaccat agacgtgctc acgctcggac tggtccccgg caacgtgagc
     8521 ctcgtgctgc tggcgacgct ggagtgcgtg atcggcctcg gtctcatctc cggcggattc
     8581 atgcgtctcg tcctgctgct cctggccttc cagatggtgg gggcggtctc tccgctcttt
     8641 atcttccccg gcgaggtgtt cacgaaggtc ccatatgcgc cgacactgga ggggcagtac
     8701 atcatcaaga acatcgtgct gatcagcgcg gggctcgtga tcggcgctac cgtccgcggg
     8761 ggcgggctcg tcgcgcatcc cgaggcggcg gagaggggaa gggaagcgga ggagcgggag
     8821 aaacagaggc acggctagag cgcagccaga gcttactcgc cggagattag gacctccagc
     8881 gtgcgaggtc cgtgtacgcc ctcgacccgg ttcagctcta tgtccgaggt cgccgacggc
     8941 ccggagatga aggttacggc ccggcgctcg tacctgaccg tatcttccag tcccgcgaag
     9001 gcttcgggga cgagacccac gatctggtcc tcccgcacca cgcacaggtg gtagtccggt
     9061 agcagcgtga gcgcccgccg tccctgcgcg gctccggcgt cgaggacgat ggtgccggtc
     9121 tgggcgatgg cgagagcgca gccggtgagc acgccgtcgc tcccgtccag ctcttcgttc
     9181 gtgagccggg gacgggccgc gtcgcgcagg gtctcgacgc cgcccggtat ccactccccc
     9241 ggcaggttcg gcgggatgac gagcttctcc acgccgcgct gtttcagggc ctcggctatg
     9301 gctccaggca gttcagactc gctcacgcgc cggacggcgg ccttgtactc ggcggccctc
     9361 tcggcgaacc gcgcgacgat ctcctcgcgc gaggcttcgc cttccttgcg gtagccgcgc
     9421 tcgacggcca cgtcctcggg acgctcgttc tccggcacgt cgcgggtcgc gcggcggatt
     9481 cgccagagga tcgtctcttt ggctgtggcc atcagcgtgc cccccggttc tgcttccacc
     9541 actcgcggaa agattgctcc gggatggcgc gcaggtcgcg ggtctcggtc catccggcga
     9601 ggcgtccggg gaggcggtgg atggagccgc tgcgggcgag cggccactgg ccgatgcggg
     9661 cgagcttctg cgcctgctcg aaacgctccg ggtcggagaa gaccttcgcg accgcctgca
     9721 tcgcgacgtt ctccgggtcg agcgtggact tcaggccgcc ggtgtcctgc ttcttacgga
     9781 cgaccctgcc gcgaaggtgg atcaggacct ggggaatgtt tatcttgacc ggacacactt
     9841 cgtagcacgc cccgcacaga ctcgaagcat acggcagaga gtccgggccg ggcttgccga
     9901 cgcccactag ctgcggcgtg aggatcgcgc cgatgggacc ggggtagacg gagttgtagg
     9961 cgtggccgcc gacccgctcg tagaccgggc agacgttcag gcacgccga
//
LOCUS       DASSMB010000120        18837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_533951, whole genome shotgun sequence.
ACCESSION   DASSMB010000120 DASSMB010000000
VERSION     DASSMB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 18837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18837
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533951"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..967
                     /locus_tag="VFJ72_07435"
     CDS             152..967
                     /locus_tag="VFJ72_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET7479332.1"
                     /translation="MKLFVLDNGALDLDLMWLVLNPTPATVDNKNPDNQWVTVPTYTV
                     LIEHPQLGYVLYDGTCHPEWDQRWPEALRPVFPYIAEEDQYLPHQLEKLNLKMSDIDI
                     VIISHLHLDHCGCLDFFRGTKAGRRGIIVHDKELSHSLKVTHTGPEDFAGAYVKGEFD
                     MPDLNYELMVGPHLELAPDLHLHHWPGHTPGLIGMVAHTEHSGTFIFTSDAIYLERNL
                     KDWVLSGIVYDTVAYFNSMYDIKQLARKHDATLGFGHDMEKFQSFKKAPEYYE"
     gene            complement(1001..1615)
                     /locus_tag="VFJ72_07440"
     CDS             complement(1001..1615)
                     /locus_tag="VFJ72_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7479333.1"
                     /translation="MMPPDERSSASSGGDAPGRQLMRRDERRGQILAAAKRAFLRSGF
                     AATGLEDVASHAEITKVLIYRHFDSKADLYRAALNDTRARVRSATGGADRLGEESFGA
                     LVAVADEDPDGFRLLYRHAVREPEFRAYAQEFRDRAATVAERYLRELVPDAGRRQWAA
                     RLVPVLTLETIMSWLDAGRPAPEEEVAVAVNGSIDGFLAAVAAE"
     gene            complement(1612..2448)
                     /locus_tag="VFJ72_07445"
     CDS             complement(1612..2448)
                     /locus_tag="VFJ72_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479334.1"
                     /translation="MDVSENGMQPALPGPGRVLLDQFGYQLRLLSRAPRAAFSAFLLP
                     VLLLAAFHLSGSYSGSAGLALVGGLTAFGVIMTGYATHAGGVVAARERGVLKRLRGGP
                     LPPWAYFSGSILATTTLCLLSATVTLLVARMLGTQVRLAEVPALFLVTLLGALSWAAL
                     GTAVTAFIPDVGSAGPLLSATYLPVVFVSGVFVPVAFEPGWLVTLASWLPAEPFIHAL
                     SAVLKTGGPGIPMPPAGDVLGSALWAVVGIYVALRHFRWEPRGGEHKGASGLAEKKAV
                     AR"
     gene            complement(2456..3394)
                     /locus_tag="VFJ72_07450"
     CDS             complement(2456..3394)
                     /locus_tag="VFJ72_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479335.1"
                     /translation="MEAKAEAKAISVSGLTMSYGGHVALVGVGFEVGVGETVAILGPN
                     GAGKTSTVEILEGFRRRSGGQVSVLGEDPGEAGRSWRARIGLVLQATSLDEQLTVREE
                     LSLYAGMYPNPKPVDEVLDLVGLAGEADRRIGTLSGGQQRRVDLGLGIVGSPELLFLD
                     EPTTGFDPAARHAAWEAIERLCAGGMTVVLTTHYLEEAERLADRVVVVADGRVVADAS
                     PGEVGGLAGRGSLVRFPVPEGVGLPANLAADATLEDGWATLATDDLAPVLGELLDWSR
                     EREVDLNRLTVGRPTLEDAYLALTTGMNTGGSHEGA"
     gene            complement(3567..4859)
                     /locus_tag="VFJ72_07455"
     CDS             complement(3567..4859)
                     /locus_tag="VFJ72_07455"
                     /EC_number="1.1.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047988 - hydroxyacid-oxoacid
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid-oxoacid transhydrogenase"
                     /protein_id="HET7479336.1"
                     /translation="MSAQGNGSSGNETIFTMEATPLKYGPGASEEVGWEAKRLGMKRI
                     MLVSDPGVVKANITGRIQELIEAEGIEVEVWDRSRVEPTADSFQAAADFAVEGNFDGF
                     VAVGGGSSIDTAKVSDLISTHGGEIIEYVNPPVGGGKKPPSPLKPLLAIPTTSGTGAE
                     ATTVAILDIPDQKVKTGISHAYMRPSRGIVDPLLTMTMPSEVTSSCGLDVVCHAAESY
                     LTKPYNERPKIDNPDDRPPYQGANPIADMWSAKALEYGGKYLRRAVENGEDTEARGAM
                     MLGASLAGIGFGSAGVHIPHACAYPIAGLKHEWQPPGYPTDHPFVPHGWSVIVTAPAS
                     FRFTYDAMPERHREVAELLAGEKIEKHDENTLPEIVIQLMKDVGAPSGVRVLGYSEDD
                     IPDLVDGAMKQQRLLVGSPKEVTEEDLANILRESMENW"
     gene            5033..6715
                     /locus_tag="VFJ72_07460"
     CDS             5033..6715
                     /locus_tag="VFJ72_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7479337.1"
                     /translation="MSSSIYERKPWLDLYADYVPKELPIPEKSMIELFEDSVARVPDR
                     DAVRYFDTTISFAELDRMAEAFAARLSEWSIEKGDRVVVYTQNNPQFLIAQYGAWKLG
                     AVVVPLNPMLKARELDYHLNDSGAKVLVCLESLYRDVAKEVLPNTPVEHVVTTSELDL
                     LPEDAAETLSALKGAKKLRFDETEDLAEIVSGAAGSSVPREPVSPDDIAYLVYTSGTT
                     GKPKGAVELHKNIAFNAEVYRTWMQIGDDDSVFGVAPLFHITGIVGHTALAALAGVPL
                     VLFHRFDPAEALRLIEKWRPTMTIGAITVFIALINTPGSGDRDLSSLEKCYSGGAPIA
                     PSITEQFEKKFGVYIHNAYGLTESNSPTHFVPWGKRAPVDEASGALAVGVPVPNCEAR
                     LLKIEDPNEEVPPGEPGEFAAKGPMIFAEYWNKPEETEKAFHDGFFLTGDVAIMDEEG
                     WFYIVDRKKDMINAGGYKVWPREVEDVLYTHPAVKEAAVVGVPDEYRGETVKAFVALK
                     EGENVTEEDLIQYSKEQMAAYKYPRQIEFLDEIPKTATGKFLRRELREGAPT"
     gene            6896..7894
                     /locus_tag="VFJ72_07465"
     CDS             6896..7894
                     /locus_tag="VFJ72_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HET7479338.1"
                     /translation="MTEERGAPSVMDPTEMWRQWYEASTKMWSQMAQGAQGNYMDPFG
                     LYQRWFDGLRQMQQRMSAVFPAAVLSSNGGSGAPAAQDLWQRWSEATADSWQKGAELG
                     QNALEILPRWMQMLEEAQSNLLASGTPATDPLQLATQWYNATSGPFSDFVGDVIEREE
                     FLEPSSRFLQNYASFYKVYKRNSEEYLKNLQLPVRSDITRVAGLIVNLEDKVDRIEEV
                     LEDFEYGNAEPATAESVQALEDRLDRVEGKLDRLLTALENNIQNGAQNAVANAPANAP
                     TSVQQVAETNGSPVEVRATDAARRKAREMGVDLATVSGTGADGQVTVDDVRKKGDS"
     gene            7895..8995
                     /gene="phaC"
                     /locus_tag="VFJ72_07470"
     CDS             7895..8995
                     /gene="phaC"
                     /locus_tag="VFJ72_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III poly(R)-hydroxyalkanoic acid synthase
                     subunit PhaC"
                     /protein_id="HET7479339.1"
                     /translation="MTETANYGEAPTTHEGVVPADVENFFDKYSKGMKIVVEGAQADT
                     GQTPKETIWTKNKAKLYHYAPNAEKTHPVPILMVYALINRPYILDLMPGNSFIEYLVN
                     EGFDVYMLDWGIPGDEDKDLDFENYVLDYIPRAVKKVLKHSGSDEFTLLGYCMGGTMS
                     AMYASLFPEHLKNLILLTAPVSFPTGEMGLYSLFTSEKYMNPGLLADAFGNIPGDLID
                     TGNRMLKPVTNYVGTYVNMWERIFEEKPMETWLAMNKWVNDGPPFPGATFRQWISEFY
                     QQNKLAKGEIVLRGRRVDLSNIKVPLLNIAGKKDHICTLPQAEATMDLIGSEDKEFFV
                     LDAGHVGLMTGRGAKKGLWPKISGWLAERSGA"
     gene            complement(9069..10994)
                     /gene="phaC"
                     /locus_tag="VFJ72_07475"
     CDS             complement(9069..10994)
                     /gene="phaC"
                     /locus_tag="VFJ72_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HET7479340.1"
                     /translation="MSEGKTGNGGSSTPSPMAPFEMWSEWMRANMGAMSATPGASIPW
                     LASPGVSTGDETSRLPRGAIANDPLLSAMEKLWDANPMQNVLPINWIEITKALQTLWA
                     REMSDPQRAFERATDFNAQVWGATMEAWRDSATRFWGLPTEQKKEEGRTDKRFSAPEW
                     ESNPFYQTLKQSYLLASEYLLKEAEETDGDNTEEQRRLKFHLQQFVDAMAPVNYLLTN
                     PAALKRAAETGGASLAEGTRNLLSDLQEGRMSMTDATAFQPGENLAITPGKVVYRNEL
                     IELIQYEPQTEKVHETPILFFPPWINKYYILDLTPKNSMVNYLVSEGYTVFMTSWKNP
                     DASMEDTKFEDYMTKGPLAAVEAVKDITGSEKVNPVGYCVGGTLLAVTIAWLAAGGDE
                     NPFHAPTFMVSLQDFDDVGDTAVFMDEPQVEFMEQQMMERGYLDNRKMANMFNLLRSN
                     DLIWSNVVNNYLLGQKPPAFDLLYWNADGTRMARDAHSFYIRNTYLENNLIKPGKVEV
                     KGRPIDLGKIEGDLYAVGAEKDHIVPWYSAWKVGQVTNATTRFALANSGHIAGMINPP
                     SKGKGKHWVNEDGDAGSAKSAEEWRANATEHEGSWWEDWTNWLEPRSGEKVDPPKMGS
                     DNYPPIEDAPGTYVKEK"
     gene            complement(11104..12000)
                     /locus_tag="VFJ72_07480"
     CDS             complement(11104..12000)
                     /locus_tag="VFJ72_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7479341.1"
                     /translation="MPVMITDETSIDYDIHGEGPPLVLINGLGFGRWGWFKQIPAFSR
                     HFRTITFDVRGERGLKNGVADLADDVVALMDQLRVRKAHVLGTSLGGFVAQELALRRP
                     DLVDRLILVCTSYGGSGPETMSPRALADMVGWGTFSPEGTVRRGLETATSDIYREEHP
                     EEFEQVVRWRLADSPSTSAYYEQARAGARFDLSGDVEHITSPTLVIHGAEDRYIPVAN
                     AAALAGAIPGAKLRVLDAAGHLVFIERFADVNREVVTFLKPRRPRDRYGKRGLHAGEK
                     GDWFAEVRRTFRRLVKKARSGI"
     gene            12235..13500
                     /locus_tag="VFJ72_07485"
     CDS             12235..13500
                     /locus_tag="VFJ72_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7479342.1"
                     /translation="MTSTPERKGSAVRPVDFIGAERLLSGEERQVRDRVREFVDREVI
                     PVAADHWDRAEFPFDLLPGLGELGLMGGCFSEEYGCAGWSNVAYGLAIAELARGSGSV
                     STFLHVQSGLAMAAIHELGSEEQKQRWLPGMAKCEKIGCFGLTEPDAGSDPGSLATTA
                     VEKDGGYLLNGEKKWIGNASFCDVAVIWARTEDGKLSCFLIEGDNPGFHTEVLPRKAS
                     QRAVWQTHITMKDCHVPKDARLPGAKGLGSTLSVLTHSRYGVGWDGLGQAMDCYEVAL
                     SYAKERTQFGAPIASFQLVQQKLVTMVNEISLSQLLSIHMGRLKDAGELDPATVSMFK
                     MNNVAKARTTAALARDVLGGNGILLDYRVMEHMADIEGVYTYEGTNDVNTLIVGQAIT
                     GHRAFSYRPPVSPEPNGNSGEPRAEEQAR"
     gene            complement(13508..14074)
                     /locus_tag="VFJ72_07490"
     CDS             complement(13508..14074)
                     /locus_tag="VFJ72_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479343.1"
                     /translation="MFYRLRLAGPFGIGFTTVSLFGLYALLYGSRSWTALLWGTGGAI
                     VAAASLVALVWISAHSPALTGTDNGEPYPFVGVSVLLVWWVRPVGIMVFGVAAMLADL
                     KGPWEYVLFAIGLLETPLPALALRSLTGTNTGVGWPALVFGVPGMQAGLIGVLAWLVL
                     AGALYASWHELDRRAARRRRDAMYFPGR"
     gene            14336..14761
                     /locus_tag="VFJ72_07495"
     CDS             14336..14761
                     /locus_tag="VFJ72_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479344.1"
                     /translation="MEDLEMTPYDAELRISEEEARSIATLLRYTVSLNTTAGLLSPGE
                     RRLATPTYRAVELAALVLEGKTFNEALEGAAQTWTGSLEHDHQHALEMLEAHRELLKQ
                     AMSGRLRPEQLAEYLEVSSEQFLELVRAGVSNPGDRLDV"
     gene            complement(14877..16529)
                     /locus_tag="VFJ72_07500"
     CDS             complement(14877..16529)
                     /locus_tag="VFJ72_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET7479345.1"
                     /translation="MLREKRPWISIYEGKLTDEPIGGSLTEFLEGAVEKYRENVALTQ
                     GERRISYGELLSLTQKTAAALSEAGVRKGDRVGLMLPNCPEYVMGFFGALKAGAAATQ
                     VNPLYVGRELEHIFNNSGTGTLIVHAMMYGKVKDVQGSTPIGRVICVGEPEGGLADGD
                     VSFEEFLDSASGSAPEVEIDPEEDLASIQYTGGTTGVSKGAMLTHANLLGGVKQTMDL
                     LIEDPKDFPENGKVIAVAPFFHIFGMTMVLLFGLRMGWNLVTVARFQPDKVMEMIRDE
                     EPVMLPGVATLFVALHNYPDMEKYGLDRVRLFTSGGASVPLGLLRSFEERTGRPIWEG
                     YGLSEGAPVSFNTYLRGSVGGSIGVPIPGTDVRIVDIETGEREVPPGEPGELVVRGPQ
                     VMRGYWNMPEETANTLRNGWLYTGDIARMDESGYLYIVDRKKEVINAAGFKVYPREVE
                     EVLYEHPEVVEGVAVGVPDEYRGETVKAFVVRKEGSALTEEELISYCKENLAPYKVPK
                     LVEFRDELPKSAVGKLLKRVLADEEREKAGLAPKTGKGVEGQ"
     gene            complement(16558..18159)
                     /locus_tag="VFJ72_07505"
     CDS             complement(16558..18159)
                     /locus_tag="VFJ72_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HET7479346.1"
                     /translation="MPRTGFRLELDGRSLKIEDVVAFARGRGDTECILSAESMEKIEA
                     SCALKQDLISRGIPIYGVTTGFGDSANRQISPSRTVRLQQNLLRFLGCGTGQLASPEV
                     TRAAMLLRANCLAKGNSGVRLELVERLLFYLNNDMLPLIPERGSCGASGDLVPLSYLG
                     SALVGEGEVLYNGEVRDSKELLEDLGLEPVELEAKEGLALTNGTSFMSAFAALAVWDA
                     EELAFVSDLCTAMASEALLGNRGHFNAFIHENKPHPGQVESARIIREMLADSRLSLDT
                     EEIFSGDGLGGKEFLKLERQVQDKYSIRCAPNVNGALRDTLAWVRSLVEVEINASDDN
                     PLFDAETGRAQSGGNFYGSHITQAMDALKIAVANLCDLMDRQLELVVDEKFNMGLTAN
                     LTPFFEEDGPEAGLHHGFKGMQLCSSAMTAEALKLSNPASVFSRSTEAHNQDKVSMGS
                     IAARDARAIVELAQNVAAIHLLAVCQALDLRGVKKASPRTRIARDLVRTRVPFLDGDR
                     RMDRDIEAVVDLIRSGALSNTMLSE"
     gene            complement(18178..18579)
                     /locus_tag="VFJ72_07510"
     CDS             complement(18178..18579)
                     /locus_tag="VFJ72_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479347.1"
                     /translation="MGTADICTVDGSECEAQDGMHFMGQGFAIPEMADSATGRSIVPV
                     RGAMGEMVYTAIHRECTPLIRSRMNDIIEVVDDAPSRAAGRGSPLPHRRPGVNRSRSR
                     TVPSGREGVEYYLTSRSVGDLDLSEHDKRQG"
BASE COUNT         3464 a         6340 c         5723 g         3310 t
ORIGIN      
        1 cgaccttccc attcacctcg cgtacctcct tctcggcttc ccctgccgta aaacaggaag
       61 tatatacaag ctccggtttg ccgcaggaat agcctcacct atataatgct aagcctggaa
      121 tcaaggggaa agtaagggaa aggagtacgg cgtgaagctg ttcgtgctgg ataatggggc
      181 cctggatctg gacctgatgt ggctggtcct gaatccgacg cccgccacgg tggacaacaa
      241 gaacccggac aaccagtggg tcacggtccc cacctacacc gtcctgatcg agcacccgca
      301 actcggttac gtcctctacg atgggacctg ccacccggag tgggaccagc gctggcccga
      361 ggccctgcgg ccggtcttcc cgtacatcgc ggaggaggac cagtacctgc cccaccagct
      421 ggagaagctg aacctcaaga tgagcgacat agacatagtg atcatctccc acctccacct
      481 cgaccactgc ggctgcctgg acttcttccg cggcacgaag gccgggcggc ggggcatcat
      541 cgtccacgac aaggagctgt cgcactcgct caaggtcacc cacaccggcc cggaggactt
      601 cgccggcgcc tacgtcaagg gcgagttcga catgccggac ctcaactacg agctcatggt
      661 cggtcctcac ctggagctgg cccccgacct gcacctccac cactggccgg gacacacccc
      721 cggactgatc gggatggtcg cccacaccga gcactccggg acgttcatat tcacctcgga
      781 cgccatctac ctggagcgca acctcaagga ctgggtgctc tccggcatcg tctacgacac
      841 cgtcgcgtac ttcaactcca tgtacgacat caagcaactc gcccgcaagc acgacgccac
      901 cctcggcttc ggacacgaca tggagaagtt ccagtccttc aagaaagccc cggagtacta
      961 cgagtaggcg tcctcccgaa cacccggaac tacgctaata ctactccgcg gccaccgctg
     1021 ctagaaaacc gtctatggag ccgttcacgg cgaccgcgac ctcctcctct ggtgcgggtc
     1081 gccccgcgtc gagccaggac ataatagtct cgagcgtgag gaccgggacg agtctggccg
     1141 cccactgcct tcgtccggcg tccgggacca actcgcgcag gtagcgctcg gcaaccgtcg
     1201 cggcccggtc ccggaactcc tgggcgtagg cccggaactc gggctcccgg acggcgtggc
     1261 ggtatagcag ccgaaagccg tccgggtcct cgtcggcgac cgccacgagc gcgccgaagc
     1321 tctcctctcc cagccggtcg gctcctccgg tagccgacct gacgcgggcc cgggtgtcgt
     1381 tgagggcggc ccggtagagg tccgccttgg agtcgaagtg ccggtagatg agcaccttgg
     1441 taatctcggc gtggctggcg acatcctcca atccagtggc ggcgaaccca ctccgcaaaa
     1501 acgcgcgttt ggccgccgcg aggatctgcc cccggcgttc gtcccgccgc atcagttggc
     1561 ggcccggcgc gtctcctccg ctgctcgcgg acgaacgctc atcaggaggc atcatcgagc
     1621 cacggccttc ttttccgcga gtcctgaggc ccctttatgc tcgccgccgc gtggctccca
     1681 ccggaagtgg cgcagggcaa cgtagatccc gaccacagcc cacagcgcgc tgcccaagac
     1741 gtcacccgcc ggcggcatcg ggatgccggg accaccggtt tttaggacag cgctcagggc
     1801 gtggatgaag ggttcggccg gcagccagct tgccaaggtg acgagccagc cgggctcgaa
     1861 ggcgacggga acgaagaccc cggagacgaa caccacgggc aggtaggtcg ccgagagcag
     1921 cggtccggcg gacccgacgt ccgggatgaa ggccgtgacc gcggtgccga gcgccgccca
     1981 ggagagcgcg cccaggagcg ttaccagaaa cagggcgggg acctcggcga ggcgcacctg
     2041 cgtacccagc atccgggcca cgaggagggt gacggtagca ctgagcaggc acagggtggt
     2101 ggtggcgagg atgctcccag agaagtaggc ccagggcggc agcggaccac cgcgcagcct
     2161 cttgagcacg ccccgctctc gggcggcgac cacccctccg gcgtgcgtcg cgtatccggt
     2221 catgatgacc ccgaaggcgg tgagtccccc gacgagggct aagccggcgc tcccggagta
     2281 cgaccccgac aggtggaagg ccgcgagcaa gaggacgggg agaaggaacg ccgagaaggc
     2341 ggcccgcggc gcacgggaga gtagtctcag ctggtagccg aattggtcga gcaggacacg
     2401 ccccggtccg gggagcgccg gctgcatccc gttttcagaa acgtccatcc gtggatcatg
     2461 cgccctcatg tgatcctccg gtgttcatgc cggtggtcag ggcgaggtag gcgtcctcaa
     2521 gggtgggccg accgacggtc agccggttca ggtccacctc tcgttctcgc gaccagtcga
     2581 gcaactcccc gagcaccggg gcgaggtcgt ccgtggcgag cgtcgcccag ccgtcttcca
     2641 gagtggcatc cgcggccagg ttcgccggta acccgacgcc ttcaggaacc gggaagcgga
     2701 cgaggctccc gcgcccggcc aacccgccga cctcgcccgg ggaggcatcg gccaccaccc
     2761 ggccatccgc caccactacc acgcggtccg ccagtctctc cgcttcttcc aggtagtggg
     2821 tcgtgagcac aaccgtcatg ccaccggcgc acaaccgttc tatcgcctcc caagccgcgt
     2881 ggcgcgccgc cggatcgaag cccgtggtcg gctcgtccag aaagagcaac tccgggctac
     2941 cgacgatccc gagtccaagg tccacccgcc tctgctgccc ccccgaaagg gtgccgatgc
     3001 gccgatcggc ctcgcccgcg agaccgacca ggtccaggac ctcatccacc ggcttcgggt
     3061 tcgggtacat gcccgcgtac agggagagct cctccctgac cgtgagttgc tcgtccaggc
     3121 tcgtcgcctg aaggaccaag ccgatccgtg cccgccagga gcgtccggcc tccccagggt
     3181 cctcgccgag aacgctcacc tgcccaccgc tgcgacgccg aaacccctcc aggatctcca
     3241 ccgtgctcgt cttgccggcc ccgttgggtc cgagaatcgc taccgtctcg cccaccccga
     3301 cctcgaatcc cacgcctacc agcgcgacgt gcccgccata ggacatcgtc agaccagaca
     3361 ccgagatcgc cttcgcttcc gccttcgcct ccatcttcct ccgttctcgg gattgtgttt
     3421 accacaaagt atatacttat aagtaaacag tttccatccg agatattgac gagggtgtta
     3481 tcgcggaaaa gcgtcaaaat ccagaaaggg gtgacggtct ttgagccgtc gccccttccg
     3541 ctacttggtg tagagaaagg tctctgctac cagttctcca tagactctcg caggatgttc
     3601 gccagatcct cctccgtgac ctccttcggg gagccgacga gcaggcgctg ctgcttcatc
     3661 gccccgtcca cgagatccgg gatgtcgtcc tcgctgtagc ccagcacgcg cacgccgctc
     3721 ggagcgccga cgtccttcat tagctggatg acgatctccg gcagcgtgtt ctcgtcgtgc
     3781 ttctctatct tctcgcccgc cagaagctcc gcgacctccc ggtggcgctc cggcatcgcg
     3841 tcgtaggtga accggaacga ggccggggcg gtcacgatga ccgaccagcc gtggggcacg
     3901 aaggggtggt cggtcgggta gccgggcggc tgccactcgt gcttgaggcc cgcaatcggg
     3961 taggcgcagg cgtgcgggat atggacgccc gccgaaccga acccgatccc cgcgagcgac
     4021 gccccgagca tcatcgcgcc gcgcgcctcc gtgtcctcgc cgttctccac ggcccgacgc
     4081 aggtacttgc ccccgtactc cagggccttc gcgctccaca tgtccgctat cgggttagcc
     4141 ccctggtatg gcggccggtc gtcggggtta tctatcttcg ggcgctcgtt gtacggcttc
     4201 gtcaggtagg actcggcggc gtggcagacc acgtccagcc cgcaggatga cgtaacctcg
     4261 gagggcatcg tcatggtcag cagcgggtcc acgatgcccc ggctcgggcg catgtaggcg
     4321 tgggagatgc cggtcttcac cttctgatcc gggatgtcga ggatggcgac ggtcgtggcc
     4381 tcggcgccgg tcccggaggt cgtcgggatg gcgaggagcg gcttcagggg cgagggcggt
     4441 ttcttgccgc cgccgacggg cgggttgacg tactctatga tctcgccgcc gtgcgtggag
     4501 atcaggtcgg acaccttcgc cgtgtctatg ctggacccgc cgccgaccgc gacgaaccca
     4561 tcgaagttgc cctcgaccgc gaagtccgcc gccgcctgga acgagtccgc ggttggctcc
     4621 acgcgggagc ggtcccagac ctcgacctct ataccctcgg cctctataag ctcctggatg
     4681 cgccccgtaa tgttagcctt caccacgccg gggtcggaga cgagcatgat ccgcttcatc
     4741 cccagccgct tcgcctccca gccgacctcc tccgaggctc ccggcccgta ctttagaggc
     4801 gtcgcctcca tcgtgaagat ggtctcgttg cctgaactgc cgttgccttg cgcgctcatt
     4861 cggcgtcttc ctcctccaat ccgcttaccc ggctctcatg ctagctacgc caccggccag
     4921 cggcaacccg aaccgcggcg cccgccgggc tcccgccggt tgacacggcg cggtacgcaa
     4981 gaaacaatac aacctctggg gaatcggata attcaggaaa gaggaggttc tcatgtcgtc
     5041 ctccatatac gagcgtaagc cgtggctgga tctctacgcg gactacgttc cgaaggagct
     5101 tccaataccc gagaagagca tgatcgagct cttcgaggac tcggtagccc gcgtcccgga
     5161 ccgcgacgcc gtccgctact tcgacaccac catctccttc gccgagctcg accggatggc
     5221 cgaagccttc gccgcgcgcc tctctgagtg gagcatcgag aagggggacc gggtagtcgt
     5281 ctacacgcag aacaacccgc agttcctcat agcccagtac ggcgcctgga agctcggagc
     5341 cgtcgtcgtc cccctcaacc cgatgctcaa ggcccgcgag ctcgactacc acctcaacga
     5401 ctccggcgcg aaggtgctcg tctgcctgga gagcctctac cgcgacgtgg cgaaggaagt
     5461 cctgccgaac accccggtcg agcacgtcgt aaccaccagc gagcttgacc tgctgccgga
     5521 agacgcggcc gagaccctct ccgccctgaa aggcgcgaag aagctccgct tcgacgagac
     5581 ggaggacctg gcggagatcg tctcgggagc ggcggggagt tcggttcctc gtgagccggt
     5641 ctcgccggac gacatcgcgt acctcgtcta cacctccggg acgactggga agccgaaggg
     5701 cgcggtcgaa cttcacaaga acatagcgtt caacgcggag gtctaccgta cctggatgca
     5761 gataggcgat gacgactccg tcttcggggt tgccccgctg ttccacatca cggggatcgt
     5821 cgggcacacg gccctggcgg cgctcgccgg ggtcccgctg gtgctgttcc accgattcga
     5881 tccggcggag gcgctgcgcc tgatcgagaa gtggcggccg acgatgacca tcggggcgat
     5941 cacggtcttt atcgccctga taaacacccc cggctccggc gaccgggacc tctcctcgct
     6001 ggagaagtgc tacagcggcg gcgccccgat agccccgagc atcaccgagc agttcgagaa
     6061 gaagttcggc gtctacatcc acaacgccta cggactcacc gagagcaact cccccaccca
     6121 cttcgtgccc tgggggaagc gcgcccccgt ggacgaggcg agcggcgcgc tggctgtcgg
     6181 cgtccccgtc cccaactgcg aggcgcgtct tctcaagatc gaagacccca acgaggaagt
     6241 gcctcccgga gaacccggcg agttcgccgc caagggaccc atgatcttcg ccgagtactg
     6301 gaacaagccc gaggagacgg agaaggcctt ccacgacggc ttcttcctca ccggagacgt
     6361 cgcgatcatg gacgaggagg gctggttcta tatagttgat cggaagaagg acatgatcaa
     6421 cgccggcgga tacaaggtct ggccgcgcga ggtcgaggac gtcctctaca ctcatcccgc
     6481 cgtcaaggaa gccgccgtcg tcggcgtccc ggacgaatat cggggcgaga cggtaaaggc
     6541 gttcgtcgcg ctcaaggaag gcgagaacgt caccgaggaa gacctgatcc agtacagcaa
     6601 ggagcagatg gccgcctaca agtacccgcg ccagatcgag ttcctggacg agatccccaa
     6661 gaccgccacc ggcaagttcc tccggcgcga actgcgcgaa ggagcgccaa cctagcagta
     6721 agctccccgg agcgcggccg ctttctgtcg taaagccgcg ctccggtata tgccgacaac
     6781 atataaatcc tgtttcttag tataattgcc gtaataaggc atcgttattc ccgtatgaat
     6841 agagatgccg ccgtataaga tgtcgcgcgt caagcatgac aaggaggttt tgaggttgac
     6901 ggaggagaga ggagcgccgt ccgtaatgga cccgacggag atgtggcgtc agtggtacga
     6961 ggccagcacg aagatgtggt cgcagatggc ccagggagcc cagggcaact acatggaccc
     7021 gttcggcctg taccagcggt ggttcgacgg cctgcggcag atgcagcagc ggatgagcgc
     7081 ggtgttcccg gcggccgtac tgtcgagcaa cggtggttcc ggcgctccgg cggcccagga
     7141 tctctggcag cgatggtccg aagctaccgc cgatagctgg cagaaaggcg ccgagttggg
     7201 gcagaacgcg ctggagatcc tgccgcgctg gatgcagatg ctcgaagagg cgcagagcaa
     7261 cctgctcgcc tccggcaccc cggccacgga tccattgcag ctcgccaccc agtggtacaa
     7321 cgccacgagc ggtcccttct cggacttcgt cggcgacgtc atagagcgcg aggagttcct
     7381 ggagccgtcc agccgattcc tgcagaatta cgccagcttc tacaaggtct acaagcgcaa
     7441 ctcggaggag tacctcaaga accttcaact tccggtccgc tccgacataa cgcgggtcgc
     7501 gggtctcatc gtgaacctgg aagataaggt agaccgtatc gaggaagtcc tcgaagactt
     7561 cgagtacggt aacgcagaac cggccaccgc cgagtccgtg caggcgctcg aagaccgtct
     7621 cgaccgggtc gagggcaagc tcgaccggct actcaccgcg cttgagaata atattcagaa
     7681 cggcgcgcag aacgctgtgg cgaacgcccc ggcgaacgcc ccgacgagcg tgcagcaggt
     7741 tgccgagacc aacggctctc cagtcgaggt ccgggcgacg gacgccgccc ggcgcaaggc
     7801 gcgggagatg ggtgtggatc tggcaaccgt cagcggcacg ggagcggacg gtcaggtcac
     7861 ggtagacgac gtgcgtaaga agggagatag ctagatgacg gagaccgcca actacggcga
     7921 ggcgcctacc acgcacgagg gcgtcgtacc cgccgacgtc gaaaacttct tcgacaagta
     7981 cagcaagggc atgaagatcg tcgtcgaagg cgcccaggcc gacaccggcc agacacccaa
     8041 ggagacgatc tggaccaaga acaaggcgaa gctctaccac tacgcgccga acgccgagaa
     8101 gacgcacccg gtcccgatcc tgatggtcta cgccctcatc aaccggccct atatcctgga
     8161 cctgatgccg ggcaacagct tcatcgagta cctcgtgaat gagggcttcg acgtgtacat
     8221 gctcgactgg ggcatccccg gcgacgagga taaggacctc gacttcgaga actacgtcct
     8281 cgactacatc ccgcgcgccg tgaagaaggt gctaaagcac tccggctctg acgagttcac
     8341 cctgctcggc tactgcatgg gcggcacgat gagcgccatg tacgcctcgc tcttcccgga
     8401 gcacctcaag aacctcatcc tgctcacggc tccggtcagc ttccccacgg gagagatggg
     8461 cctctacagc ctcttcacca gcgagaagta catgaacccc ggcctgctgg cggacgcctt
     8521 cggcaacatc cccggcgacc tcatcgacac cggcaaccgg atgctaaagc ccgtcaccaa
     8581 ctacgtcggc acctacgtca acatgtggga gcgcatcttc gaagagaagc ctatggagac
     8641 ctggctcgcc atgaacaagt gggtcaacga cggcccgccg ttccccggcg ccacgttccg
     8701 gcagtggatc tccgagttct accagcagaa caagctggcc aagggcgaga tcgtgctccg
     8761 aggacgccgc gtggacctct ccaacataaa ggtcccgctc ctcaacatag ccggcaagaa
     8821 ggaccacatc tgcaccctcc cgcaggccga ggccacgatg gacctcatcg gcagcgagga
     8881 taaggagttc ttcgtcctcg acgccggcca cgtcggtctc atgaccggcc gaggcgccaa
     8941 gaagggcctg tggcccaaga tcagcggctg gctcgccgaa cgctccggcg cgtaagagaa
     9001 gcagagccca cgacaaaggg gcggggccgc ttgcgcggcc ccgccccttt cttctgcgct
     9061 accggcgtct acttctcctt gacgtatgtt cctggggcat cttctatggg cgggtagttg
     9121 tcgctgccca tcttgggcgg gtccaccttc tcgccggagc gaggttcgag ccagttcgtc
     9181 cagtcctccc accaggagcc ctcgtgctcg gtggcgttgg cgcgccactc ctcggcggac
     9241 ttggcggaac cggcgtcgcc atcctcgttg acccagtgct tgcccttgcc cttgctgggc
     9301 gggttgatca ttccggcgat gtgaccgctg ttcgcgagcg cgaagcgggt cgtcgcgttg
     9361 gttacctgtc cgaccttcca ggccgagtac cagggtacga tgtggtcctt ctccgcgcct
     9421 acggcgtaga ggtcgccctc gatcttgccg aggtctatgg gacggccctt tacctctacc
     9481 ttgcccggct ttatgaggtt gttctccagg taggtgttgc ggatgtagaa gctgtgggcg
     9541 tcgcgcgcca tgcgcgtgcc gtcggcgttc cagtacagca ggtcgaaggc cggcggcttc
     9601 tggccgagca ggtagttgtt gacgacgttc gaccagatca ggtcgttgga gcgcagcaaa
     9661 ttgaacatgt tggccatctt gcggttgtcc aggtagccgc gctccatcat ctgctgctcc
     9721 atgaactcca cctgcggctc gtccatgaat accgccgtgt cacccacgtc gtcgaagtcc
     9781 tgcagcgaga ccatgaacgt cggggcgtgg aaggggttct cgtcgccccc agcggcgagc
     9841 caggcgatgg tgaccgccag cagagtcccg ccgacgcagt acccgaccgg gttgactttc
     9901 tccgagccgg ttatgtcctt cacggcctcg accgccgcga gaggcccctt cgtcatatag
     9961 tcctcgaact tcgtatcctc catggaggcg tccgggttct tccaggaggt catgaagacc
    10021 gtgtagccct cgctcaccag gtagttgacc atgctgttct tcggcgtcag gtcgaggatg
    10081 tagtacttgt tgatccaggg cgggaagaac agtatcggcg tctcatggac cttctcggtc
    10141 tgcggctcgt actggatcaa ctctataagc tcgttgcggt agacgacctt gcccggcgtg
    10201 atcgccaggt tctcgcccgg ctggaaggcc gtggcgtcgg tcatgctcat ccgcccctcc
    10261 tgcaggtccg agaggaggtt gcgggtcccc tcggcgaggc ttgcgccgcc cgtctccgcg
    10321 gcgcgcttga gggcggcggg attcgtgagg aggtagttga ccggcgccat cgcgtccacg
    10381 aactgctgga gatggaactt cagccggcgc tgctcctcgg tgttgtcgcc gtcggtctcc
    10441 tccgcttctt tcagcaggta ctcggaggcg agcagatacg actgcttgag cgtctggtag
    10501 aacgggttcg actcccactc gggggcggag aagcgcttgt ccgtccttcc ctcctctttc
    10561 ttctgctccg tcggcaggcc ccagaagcgg gtcgccgagt cgcgccaggc ttccatcgtc
    10621 gcgccccaca cctgcgcgtt gaagtccgtg gcgcgctcga aggcgcgctg cgggtcggac
    10681 atctcgcgcg cccagagcgt ctggagggcc ttcgtgatct cgatccagtt gatcgggagc
    10741 acgttctgca tcgggttggc gtcccacagc ttctccatcg ccgagagcag cgggtcgttt
    10801 gcgatggccc cacgaggcaa ccggctcgtt tcatcaccgg tcgaaacgcc gggagaagcc
    10861 agccagggta tgctcgcgcc cggcgtcgcg ctcatcgcgc ccatgttcgc ccgcatccac
    10921 tccgaccaca tctcgaacgg ggccatgggg ctcggggtgc tggatccacc attgcctgtt
    10981 tttccttcac tcatatagtc ttcctcctcg gactgtttgt taggacggct catgccatcc
    11041 tcttgtgtcc cgcttcacag tgtacccgtt aaccggacgc tcccaacaag ccgggcgagg
    11101 tacctagatt ccgctccggg ctttcttgac cagacggcgg aacgtccgcc gcacttccgc
    11161 aaaccagtct cccttctccc ccgcatgaag cccccgctta ccgtatctgt ctctcggcct
    11221 gcggggcttg aggaacgtca cgacctcccg gttcacatcg gcgaaccgct cgatgaacac
    11281 cagatgaccg gcggcgtcca gaactcgcaa ctttgctcct ggaatcgctc cggccagggc
    11341 ggcggcgttc gcgacgggta tgtaacggtc ctcggccccg tggatgacga gggtcggcga
    11401 ggttatgtgc tccacgtcgc cggagagatc gaagcgggct ccggcccgcg cctgctcgta
    11461 gtaggcgctg gtcgagggcg agtccgcgag acgccagcgg acgacctgct cgaactcttc
    11521 gggatgctcc tcgcgataga tgtccgaggt cgccgtctcc agcccccgcc ggaccgtacc
    11581 ttcgggacta aaggtacccc agccaaccat gtccgccaga gccctcggag acatggtctc
    11641 cggcccgctg ccaccgtagc tcgtacatac gaggattagc cgatccacca ggtccggcct
    11701 cctgagcgcc agctcctgcg ccacgaaacc accgagagag gtgcccagca catgcgcctt
    11761 cctaacccgg agttgatcca tgagcgccac cacgtcgtcg gccaggtcgg caacgccgtt
    11821 cttcaagccc cgctctcccc gcacgtcgaa cgtaatggtg cggaagtggc gcgaaaaggc
    11881 cggtatctgc ttgaaccatc cccagcgccc gaagcccagg ccgttgatca ggaccagcgg
    11941 cggtccctcc ccgtggatgt cgtaatctat gctggtctcg tcagtgatca ttacgggcat
    12001 aaaccgctca cctcgattct tccggtcccg atactacgcc gcttacgccg ctcaaccaga
    12061 ttttcgtata cggataatca cattcatatt gtatatagat gtattttgca cacaagtagg
    12121 aggtgtgaaa agcttcgcgg ataggattga ccccggtcat acgacaacat atgcttgcgt
    12181 gggagaaagg ggaattctgg ggatgtctgg agagcggatc aagaggagaa gataatgact
    12241 tccacgccag agcggaaggg gagtgcggtc cggcccgttg actttatcgg cgcggagcgt
    12301 ctgctctccg gggaggagcg gcaggttcgg gaccgggtac gcgagttcgt ggaccgggag
    12361 gtaatccctg tagccgccga tcactgggac cgggccgagt ttccgtttga tctcctgccg
    12421 ggtctcggag agttgggcct tatgggcggc tgcttctccg aggaatacgg ctgcgccgga
    12481 tggagtaacg tcgcctacgg tctcgctatc gcggagcttg ctcgggggtc gggcagcgtg
    12541 tccacgttcc tgcacgtcca gagcgggctg gcgatggcgg cgatccacga gctcggcagc
    12601 gaggagcaga agcagcggtg gcttccaggg atggctaagt gcgagaagat cggctgtttc
    12661 ggcctcaccg aacccgacgc cggaagcgac cccggttctc tcgcgaccac tgctgtcgag
    12721 aaagacggcg gttatttgct gaacggtgaa aaaaagtgga tcggcaacgc ctccttctgc
    12781 gacgtggccg tgatctgggc gcggaccgag gacggcaagc tctcctgctt cctgatcgag
    12841 ggggacaacc ccggtttcca caccgaagtc ttgcctcgta aggcctccca gcgggccgtc
    12901 tggcagacgc acatcaccat gaaggactgc cacgttccga aagatgcccg gcttccggga
    12961 gcgaaggggc tcggctcgac cctctccgtg ctcacgcact cgcgctacgg cgtggggtgg
    13021 gacggtctcg gtcaggcgat ggactgctac gaggttgcgc tatcctacgc gaaggagcgc
    13081 acgcagttcg gtgcaccgat agcgtctttc cagctcgtgc agcagaagct cgtcacgatg
    13141 gtcaatgaga tctccctgag ccagctactc tcgatccaca tgggccgcct gaaggacgcc
    13201 ggagagctgg accccgcgac cgtttcgatg ttcaagatga acaacgttgc gaaagctcgg
    13261 accacggccg cgctcgcccg cgacgtgctc ggcggcaacg gcatcctgct cgactaccgg
    13321 gtgatggagc acatggcgga tatcgagggc gtctacacct acgagggcac gaacgacgtg
    13381 aacaccctga tcgtgggtca ggccattacc ggacaccggg ctttctccta ccgccccccg
    13441 gtgagccccg aaccgaacgg caactccggg gaaccccgcg ccgaggagca ggcccgctag
    13501 cgctggtcta ccgtcccgga aaatacattg cgtcgcggcg tctcctggcg gcccggcggt
    13561 ccagctcgtg ccaggaagcg tagagagcgc cggccagcac gagccacgcc aggacgccga
    13621 tcaaacccgc ctgcatcccc ggcaccccga acacgagcgc cggccagcca acaccagtat
    13681 tagtccccgt gagagagcgc agcgcgagcg cgggaagcgg cgtctccagc agtccgatgg
    13741 cgaagagtac gtactcccac ggtcccttga ggtccgcgag catggcggcg acgccgaaga
    13801 ccatgatccc gaccggacgc acccaccaga ccagcaatac gctcacgccc acaaatggat
    13861 acggctcccc gttgtccgtc cccgtgagag cgggcgagtg cgcggagatc cagacgagag
    13921 ccacgagcga agccgccgct actatggccc cgcccgtacc ccacagtagc gccgtccatg
    13981 agcggctccc gtacagcaag gcatagagcc cgaagaggct caccgtagta aatccaatcc
    14041 cgaaaggccc ggccagccgc agcctgtaga acatccccgg ctcctccacc gtcccggacg
    14101 ctccggaagc aaaaacaagc cacaacactc cgaccccgag caaagcggct cccgcaaaag
    14161 ccgccaccgc cccgcaccat accggccacg cagtacgccc cggtccccca cggcgcacac
    14221 ccccaacctc tctcatgcaa cgcagtctaa ccgatcgagc gttcctgccg gtatggaagc
    14281 cggctcccgc aacgtctaaa atcttgccga cacctcgatg ggaagagaag gatatatgga
    14341 ggacctggag atgacaccat acgatgcgga gcttcgcatc tcagaagaag aggcgcgaag
    14401 catagcgacc ctgctccgct acaccgtcag ccttaacacg accgccggac tcctctcgcc
    14461 gggcgagcgc cgcctggcta cccccacata ccgggccgtg gaactcgccg ccctcgtcct
    14521 cgaagggaag accttcaatg aagccctcga aggagcggca cagacctgga ccggctccct
    14581 ggaacacgac catcagcacg ccctggagat gctcgaagcc cacagggagc ttctcaaaca
    14641 ggctatgtcc ggcaggctcc gccccgaaca actcgccgaa tacctggagg tcagcagcga
    14701 acagttcctg gaactcgtcc gcgcgggggt ctcgaaccct ggagaccgct tagacgtttg
    14761 atgcgtaatt gcggatcacg gagaaggagc cgaaaccttt tgctggtagc cgacattctc
    14821 ccaaaggtgg tgccccaggg atctcgactc cttcctggtc tcgttaggcg aggtcctcat
    14881 tgtccctcta ctccctttcc ggtcttcggg gccaatcccg ccttctcgcg ttcctcgtcg
    14941 gcgagcaccc gcttcaggag cttgccgacg gcgctcttcg ggagctcgtc gcggaactca
    15001 acgagcttcg gcaccttgta gggagccagg ttctccttgc agtaggagat gagttcctcc
    15061 tccgtgaggg cgctgccctc tttcctgacc acgaacgcct tgacagtctc cccccgatac
    15121 tcgtcgggga cgccgacggc gacgccctcg accacctccg ggtgctcata gaggacttct
    15181 tctacctcgc gcgggtagac cttgaagccg gctgcgttta tgacctcttt cttccggtcc
    15241 actatgtaga ggtatccgct ctcgtccatg cgggcgatgt ctccggtata gagccagcca
    15301 ttacgcaggg tgttcgccgt ctcctctggc atgttccagt agccgcgcat cacctgcggt
    15361 ccccggacga cgagctcgcc cggctcgccc gggggcactt ctctctcccc ggtctcgatg
    15421 tctactatcc gcacgtcggt gccggggatg gggacgccga tgctgccgcc aacagagcca
    15481 cggaggtagg tgttaaagga gacgggtgct ccctcggaga ggccgtagcc ctcccagatc
    15541 ggacgcccgg tacgctcctc gaaagagcgg agcagcccga gcggaacgct ggctcctccg
    15601 gaggtgaaga ggcggaccct gtcaaggccg tacttttcca tgtccgggta gttgtgcagt
    15661 gcgacgaata gcgtcgcgac gcccggaagc atgacgggct cctcatcccg gatcatctcc
    15721 ataaccttat ccggttggaa gcgagctacg gttacgaggt tccatcccat ccgcaggccg
    15781 aagagcaaga ccatagtcat cccgaagatg tggaagaacg gcgccacggc gatcaccttg
    15841 ccgttctcgg gaaaatcctt tggatcttcg ataagcaggt ccatcgtctg cttgacaccc
    15901 ccgaggaggt tggcgtgcgt gagcatcgcg cccttggaga cgccggtcgt gccgcccgtg
    15961 tattggatgg aggcgagatc ttcttccggg tctatctcta cctccggagc gctccccgaa
    16021 gcagaatcca ggaactcctc gaaggagacg tcgccatccg cgagtccccc ctcgggctcg
    16081 ccgacgcaga tcacgcgccc gataggggtg ctaccctgaa cgtccttcac tttcccgtac
    16141 atcatggcat gtacgatgag cgtcccggtg ccggagttgt tgaagatgtg ctccagctct
    16201 cgtcccacat agagcggatt tacctgcgtg gccgccgcgc cggccttcaa cgccccgaag
    16261 aaccccataa cgtactccgg gcagttcggg agcatgagcc ctacccggtc gcccttcctc
    16321 accccggcct cggagagagc agccgccgtc ttctgcgtca ggctcagcag ctctccgtaa
    16381 gagatcctgc gctcgccctg ggtcagggcg acgttctccc ggtacttctc cacggcccct
    16441 tcaagaaact ccgtcagcga accgccgatg ggctcatccg tgagtttgcc ctcgtagatc
    16501 gagatccagg gcctcttctc tcgcaacacg gcttccgcct cctcgtctcc tcttcgctta
    16561 ctctgaaagc atggtattcg acagcgcacc ggaccggatc aggtccacta ccgcctcgat
    16621 gtctctatcc atgcgccggt cgccgtccag gaacggcacc cgggtacgca ccaggtcccg
    16681 ggcgatcctc gtcctcgggc tcgccttctt tacgccgcgc aggtcgaggg cctgacagac
    16741 agccaggagg tgtatggccg cgacgttctg cgcgagctcg acgatcgcgc gggcgtcgcg
    16801 ggcggcaatg gagcccatgc tcaccttgtc ctggttgtgc gcctcagtgg agcgcgagaa
    16861 gacgctcgcc gggttcgaga gcttcagagc ctcggcggtc atggccgaag agcagagctg
    16921 catccccttg aagccgtggt gcagaccggc ctcggggcca tcctcttcga agaacggggt
    16981 gaggttggcg gtaaggccca tgttgaactt ctcgtccacg accagctcca gttgccggtc
    17041 cataaggtcg cagaggttgg cgacggcgat cttgagtgcg tccatcgcct gggttatgtg
    17101 gctgccgtag aagttgccgc cgctctgcgc ccggccggtt tcggcgtcga agagggggtt
    17161 gtcgtcggag gcgttgatct cgacctccac caacgagcgg acccacgcta gcgtatcgcg
    17221 caatgcgccg tttacgttcg gcgcgcagcg gatcgaatac ttgtcctgca cctgccgctc
    17281 cagcttcagg aactctttcc caccgagacc gtcgccggaa aagatctcct cagtatccag
    17341 cgagagcctg gaatccgcca acatctcgcg gatgatccgc gcgctctcaa cctgtccggg
    17401 atgcggcttg ttttcgtgga taaaggcgtt gaagtggccc cggttaccga gcagcgcctc
    17461 cgaagccatc gccgtgcaca ggtccgagac gaacgccagc tcctccgcgt cccacacggc
    17521 cagcgccgcg aacgcgctca tgaatgacgt cccgttggtc agggcgagtc cctccttggc
    17581 ttcgagctcc accggctcca ggcccagatc ctctagcaat tctttcgagt ccctgacttc
    17641 gccgttatag agaacctctc cctcgcccac gagtgcggac cccaggtaag agagcggcac
    17701 caggtccccg ctggcgccgc aggagccgcg ctcggggatg agcgggagca tgtcgttgtt
    17761 caggtaaaag aggaggcgct ctaccagttc cagacgaacg ccggagttgc ctttggcgag
    17821 gcagttggcg cgcaggagca tcgcggcccg cgtcacttcc ggagaggcca gttgcccggt
    17881 accgcacccc aggaagcgca gcaggttctg ctgcaagcgg acagtccttg atggagagat
    17941 ctggcggttc gcgctgtccc cgaagccggt cgttacgccg tagatcggga tcccacgcga
    18001 gatcaggtct tgcttcaagg cgcacgaagc ctcgatcttc tccatagact cagcggatag
    18061 aatgcattcc gtatcccccc tgccgcgggc aaaagccacc acgtcttcta tcttcagcga
    18121 ccggccatcg agctccagcc ggaagcccgt tctcggcaag atatctttca tacggcatca
    18181 cccctgacgc ttgtcatgct cgctcaagtc caggtcccca actgaacgag aggtcagata
    18241 atattctacc ccctctcgcc ccgaagggac ggttcgtgac cggctgcggt tcacgccggg
    18301 acgccgatgc gggagcggac ttcctcgtcc tgccgcacga gaaggcgcgt cgtccaccac
    18361 ctctattatg tcgttcatcc tggaccggat cagaggcgtg cattcgcgat gaatagccgt
    18421 gtaaaccatc tcacccatag cgcccctgac gggcactatg gaccttccgg tcgcggagtc
    18481 agccatctcc gggatggcga agccctgacc catgaagtgc atgccgtcct gcgcctcgca
    18541 ctcgctcccg tccacggtgc agatgtccgc cgtgcccatg cagtcataga ccttgtccca
    18601 ggcgtcctcg atctgccgcc cggctccgcg ccaaggccca tgtttctcag accgagccct
    18661 ttgagatcga tatcgttctc actggcgagc cccaccgccc ggcttgaccg caggtgtccc
    18721 gcgggtcggc gaccgggcct ggtcgtccct gaaacccccg tacagtcgat tcccttcgat
    18781 cttgcggcat gcgcgggtga acgcagcgaa gaggcggagc ggacagagtc cgccgcg
//
LOCUS       DASSMB010000121         6959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1673883, whole genome shotgun sequence.
ACCESSION   DASSMB010000121 DASSMB010000000
VERSION     DASSMB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1673883"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..483)
                     /locus_tag="VFJ72_07515"
     CDS             complement(64..483)
                     /locus_tag="VFJ72_07515"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET7479348.1"
                     /translation="MPLKIGAGDVGHIHDHAREGYPEEVAGAMVGMNLDGMKIVVDVW
                     RAENTHEEERSRRFLIDPLVYKRFEEQADERDMEILGFYHSHPDHPAEPSEYDRDHAW
                     PGYSYVIASVSGENVEDMRSWTLKDDRSGYDEEPIVG"
     gene            complement(486..1412)
                     /locus_tag="VFJ72_07520"
     CDS             complement(486..1412)
                     /locus_tag="VFJ72_07520"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET7479349.1"
                     /translation="MITRIKERIGTRNVDLVGNTPLFELPSISAETPGVGIYGKAEWY
                     NPGGSVKDRPAMWMIRDGEQSGALTPEKTILDATSGNTGIAYAWIGAALGYKVKLCMP
                     KNASEERKKILRAYGVDFVLTDPGEGSDGAIREARKLYAEDPERYFYPDQYSNPANPR
                     AHYESTAPEIWEQTNGEVTHFVAGLGTSGTFVGTATRLKEYNPEIRVISFEPDSPFHG
                     LEGMKHMESAIVPEIYDPTIADENRATPTEDAYEMVKRVAREEGILIGISAGAAVATA
                     LQVAREIESGTVVTILCDGADKYLSESFWEED"
     gene            complement(1590..2312)
                     /locus_tag="VFJ72_07525"
     CDS             complement(1590..2312)
                     /locus_tag="VFJ72_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HET7479350.1"
                     /translation="MTDTRTRQQQAQEEKLPAQYINYVFFKLDPLWRRLPDEERERGK
                     REFLEAAEEHSREMLLRCFSLMGLRADADFMLWRIGYDLEAFEKMTAALLKTGLGKYL
                     DVTYSYFALSRRSIYVGEHTPGFENRTHIIPGEGEYLFVYPFVKTRAWYGLPMEERQR
                     MMNEHMQIGRKHYPVKNNTGYCFGIDDYEFILSFEAESPEKFQDLMMELRESEASSYT
                     ELDTPIFTCRRRELPAVLNSLG"
     gene            complement(2319..3323)
                     /locus_tag="VFJ72_07530"
     CDS             complement(2319..3323)
                     /locus_tag="VFJ72_07530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7479351.1"
                     /translation="MKYRRLGGTDIEVSEVGFGVWTVSTGWWGEVDDDRSVRLLRQAH
                     ERGVNYFDTADTYGSGKGETLLADAFGHMRDEVVISTKIGYDFYNHTARRGQQERPQD
                     WSEDFLRFALEQSLKRLDTDYVDFLQLHNTKMDAIEDDELFALMEQFKAEGKIRAHGV
                     ALGPKIGWLDEGVRAMRERRIDGLQMIYNILEQDPGRGLIEAARETGTSLIVRVPHSS
                     GMLEGHYDENTTFAQNDHRRHRPKEWLTTGLKKVEQLEFLTESGERTLGQAALKFVLA
                     SPEVVSTLPNIYGEEQIEEFAATSETPDLTEEELARVAELYDHNFGLVDAGADAAARR
                     "
     gene            3601..4560
                     /locus_tag="VFJ72_07535"
     CDS             3601..4560
                     /locus_tag="VFJ72_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7479352.1"
                     /translation="MLRAPRRAVLVSINVMVGCLAMLLFAAGLARADQMVASWYGPGF
                     EGATTASGEPFNPNDYTAAHRTLPFGTKLIVTYNGRSVVVRINDRGPYVSGRDLDLSQ
                     AAAEYIGLTAVGEAAVSVETADPSTPTGPYSGASTTAPETPAPQPQAPQQQTSQQIPQ
                     ADTQQVSTTVSGGAAPGGKQNAGKEQYASADQYKPQTVAAPAPPPSPAPVAVAAAPPT
                     PDPSRLETPPTEVDTPGSTVERRVVLFVGTPLENTAVKASQTPEPETEPAPETASAKT
                     PPDKEIPGVTVLPDTGGAPLWAFAGGAILLGAGALTFFKIRRG"
     gene            4958..5158
                     /locus_tag="VFJ72_07540"
     CDS             4958..5158
                     /locus_tag="VFJ72_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479353.1"
                     /translation="MTITVTREKVKEVTREDVDRAIDVIERHLEKEARTRNLLDEQAI
                     MRPKDLCKQQGLDMVAELRGMM"
     gene            5498..6193
                     /locus_tag="VFJ72_07545"
     CDS             5498..6193
                     /locus_tag="VFJ72_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479354.1"
                     /translation="MPERHTNWIRIGLFALPISGALLAWSSLDPQPNQNKNPEAWARF
                     VGSTYYLLTHVFGSVGSALLAIFGVMALGAYLAKSRAGRLGLVAMVVTVLGQALSLVI
                     GGVSTFALNAIGRAYLSGTKDVMQLEFSSAMSVLFALAILLLIVGNVLLGVAVWRSGA
                     LPKWTGTIWVASALMFYVLGAVLGMATTGSSLPTQPVGGLLMVISGVWIAWSALRQPS
                     VGIVGAAAQPGMQ"
     gene            6274..>6959
                     /locus_tag="VFJ72_07550"
     CDS             6274..>6959
                     /locus_tag="VFJ72_07550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7479355.1"
                     /translation="MSTATAAPQPAPASPIRRLLIQHPLVSFFALAYALTWLAWSPWY
                     LSEDGIGFLHFDGESVSDYINTVALIVGPTLSAFIVTGATEGREGVRRLLRRIVLWRV
                     SLQWYLIVLVGIPALIVFCAIVMPGALASFKASAVPSTLFLYVVAGAVFLFAGGPVFE
                     EIGWRGFALPRLQRLYGPLVGSLILGVLWGMWHLPLFFIPSWDTPHGSPLDIALFVIW
                     AVSIAILFTWV"
BASE COUNT         1177 a         2202 c         2185 g         1395 t
ORIGIN      
        1 gtgtactgtc tcagcggggt tgggatcttg accttaggca tatctaagac tccttctctg
       61 ttcttatcct acgattggct cttcgtcgta gcccgagcgg tcgtccttga gcgtccacga
      121 gcgcatgtct tccacgttct cgccgctgac cgaggctatc acgtacgagt agccgggcca
      181 ggcgtggtcc cggtcgtact cggacggttc ggccgggtgg tccgggtgcg agtggtagaa
      241 cccgaggatc tccatgtccc gctcgtcggc ctgctcctcg aacctcttgt acaccagcgg
      301 gtctatgagg aaccggcggc tgcgctcttc ctcgtgcgtg ttctccgccc gccacacgtc
      361 caccacgatc ttcatgccgt ccaggttcat gccgaccatc gccccggcca cctcctcggg
      421 atagccttcc ctggcgtggt cgtggatgtg cccgacgtcc cccgcgccga tcttgagcgg
      481 caaggctagt cttcctccca gaagctctcg gagaggtact tgtccgcgcc gtcgcacagg
      541 atggtcacga ccgtcccgga ctctatctcc cgcgccacct gcaaggcggt cgccacggcg
      601 gccccggcgg agatgccgat caggatgccc tcctcgcgcg ccacgcgctt gaccatctcg
      661 taggcgtcct cggtgggcgt tgcccggttc tcgtcggcga tggtcgggtc gtagatctcg
      721 ggcactatcg cgctctccat gtgcttcatg ccctccagcc cgtggaacgg agagtccggc
      781 tcgaaggaga tgacccgtat ctccgggttg tactccttga gccgcgtcgc tgttccgacg
      841 aaggtgccgc tggtcccaag acccgcgacg aagtgcgtga cctcgccgtt tgtctgctcc
      901 cagatctccg gcgcggtgct ctcgtagtgc gcccgcgggt tcgccgggtt gctgtactgg
      961 tccgggtaga agtaccgctc cgggtcctcc gcgtagagct tgcgcgcctc gcggatcgcg
     1021 ccgtccgatc cctcgccggg gtcggtgagg acgaagtcca cgccgtaggc gcgcaggatc
     1081 ttcttgcgct cctcgctggc gttcttcggc atgcagagct tgaccttgta gcccaaagca
     1141 gccccgatcc aggcgtaggc gatcccggta ttgccgctcg tcgcgtccag tatcgtcttc
     1201 tcgggcgtga gcgccccgct ctgctcgccg tcccggatca tccacatggc cggccggtcc
     1261 ttgaccgagc cgccggggtt gtaccattcc gccttgccgt agatgccgac gcccggcgtc
     1321 tccgccgaga tgctcggcaa ctcaaacagt ggcgtgttcc cgacgagatc cacgttgcgc
     1381 gtcccgatcc gttctttaat cctagtaatc atgtcgggat tattatacag gcccggagtt
     1441 cagcgtccac atgggggaga ggacattcgg acaaagacta tagccgggag gcgtgcagaa
     1501 tcaccccccg gccaaggttg gtgcgatagg tggattatac ggaggccatc ttccaggcgc
     1561 tacggtgaac gtgttaacaa ttgtaaagat taccctagtg aattgagaac cgccgggagc
     1621 tctctgcggc ggcaggtgaa gatgggggtg tcgagttcgg tgtagctgct ggcctcgctc
     1681 tcgcgcaatt ccatcatcag gtcctggaac ttctcaggcg attcggcctc gaaggagagg
     1741 atgaactcgt agtcgtcgat gccgaagcag tagccggtgt tgttcttgac ggggtagtgc
     1801 ttgcgtccga tctgcatgtg ttcgttcatc atgcgttggc gctcctccat cgggagcccg
     1861 taccacgccc gcgtcttcac gaacgggtag acgaagagat actcgccctc accgggaata
     1921 atgtgggtgc ggttctcgaa ccccggcgtg tgctcgccca cgtagatcga acgccgcgac
     1981 agcgcgaagt acgagtaggt cacgtcgagg tacttgccga ggccggtctt cagcagagcg
     2041 gccgtcatct tctcgaaggc ttcgaggtcg tagccgatcc tccacagcat gaagtccgcg
     2101 tccgcccgca gccccatgag cgagaaacac cgcaagagca tctcgcgcga gtgctcctcg
     2161 gcggcttcca ggaactcgcg cttgccgcgc tcgcgctcct cgtccggcag gcggcgccag
     2221 agggggtcga gcttgaagaa gacgtagttt atgtactggg ccggaagctt ctcctcctgc
     2281 gcctgctgct gccgggttcg ggtgtctgtc attcctctct acctcctggc ggccgcgtcc
     2341 gcccctgcgt ccacgaggcc gaagttgtgg tcgtacagct ccgcgacccg cgcgagctcc
     2401 tcctccgtca ggtccggtgt ctcggaggtg gcggcgaact cttctatctg ctcctcgccg
     2461 tagatgttgg ggagggtgga gacgacctcg ggagaagcca gcacgaactt gagagccgcc
     2521 tggccgagtg tgcgctcgcc ggactcggtg aggaactcta gctgctcgac cttcttgagg
     2581 ccggtggtga gccattcttt cgggcggtgg cggcgatggt cgttctgggc aaaggtagtg
     2641 ttctcgtcgt agtggccttc gagcataccg gaggagtggg ggacgcggac tatcaggcta
     2701 gtgcccgtct cgcgggcggc ttcgatcagg ccgcggccgg ggtcctgttc gaggatgttg
     2761 tagatcatct gcagaccgtc tatccggcgc tcgcgcatgg ccctgacgcc ttcatccagc
     2821 cacccgatct tcggcccgag cgcgacgccg tgggcgcgta tcttgccctc cgccttgaac
     2881 tgctccatga gcgcgaacag ctcgtcgtcc tcgatggcgt ccatcttggt gttgtggagt
     2941 tggaggaagt ccacgtagtc cgtatcgagt ctcttcaggc tctgctccaa agcgaagcgc
     3001 agaaagtcct cggaccagtc ctgcggacgc tcctgttgac cgcggcgggc ggtgtggtta
     3061 tagaagtcgt agccgatctt ggtcgagatc acgacctcat cgcgcatgtg gccgaaagca
     3121 tccgcgagca acgtctcgcc cttgcccgac ccatacgtat ccgcggtgtc gaagtagttg
     3181 acgccccgct cgtgagcctg ccggagtagc cgcacggagc ggtcatcgtc cacctcgccc
     3241 caccagccgg tcgagaccgt ccacaccccg aacccgacct ccgagacctc gatgtccgtg
     3301 cccccgagcc tgcgatattt catcttctgc ctccgcttct ctgtacaacc gtaccttccc
     3361 gaacaactac tacattctaa cgccgcacta acgctgcgcg ctgttgactt tctctcaccc
     3421 gaagggatgc ttcgaaccgg atccctccag ccgcgcttca gcagatattt cggaaatgtt
     3481 acgaaaacat tgcggtttag tattgatact tgagaagcgt tgctttaagc tggtctccgg
     3541 ctaccgatga atctaggcgt cgggggtcga aaacaactcg gagggggttc attggctgca
     3601 atgcttagag cgccgcggcg cgcggtactc gtctcgatca atgtgatggt tggctgtctt
     3661 gcgatgttgc tattcgcggc cgggttggcc cgcgcggatc agatggtggc gagttggtac
     3721 gggcctggtt tcgagggcgc gaccacggca agcggagagc ctttcaaccc caacgactac
     3781 acggcggccc acaggacatt gccgttcggg acgaagctga tcgtgaccta caacggccgc
     3841 tcggtcgtgg tgcggatcaa cgaccgcggc ccgtacgtct cgggcaggga cctcgatctc
     3901 tcacaggccg cagccgagta catcggcctg accgctgtcg gcgaggccgc ggtgagcgtg
     3961 gagaccgccg acccttcgac cccgaccgga ccatacagcg gcgcgtcgac gacggcaccg
     4021 gagactccgg ccccgcagcc gcaagctcct caacagcaga cttcgcagca gatcccgcag
     4081 gctgatactc agcaggtgtc cacgacggtg tccggcggag cggctccggg cggcaagcag
     4141 aacgccggta aggagcagta cgcttccgcg gaccagtata aaccacagac agtggcggct
     4201 ccagctccgc cgccctcgcc ggctcccgtg gcagtagccg ccgcgcctcc cacgccggac
     4261 ccgtcccgcc tggagacgcc gccgaccgag gtagacacgc ccggctcgac ggtggagcgc
     4321 cgggtcgtgc tgtttgtagg cacgccgttg gagaacaccg ccgtgaaagc gtctcagacg
     4381 ccggaacccg agacggagcc cgctcccgag acagcctccg cgaagacccc cccggataaa
     4441 gagataccgg gtgtcacggt tctccccgac acgggcggcg ctcctctgtg ggcgttcgcg
     4501 ggaggggcta tcctcctcgg cgccggcgcg ttgaccttct tcaagatccg gcgcggctag
     4561 cctcggatag cctggtttag ccccggctca tgaaaccgga cgtccggcgg gcagtaccgc
     4621 ccggaggggc gggtcccatg tgggacccgc ccctccgcat gagtggagga gcatgagacc
     4681 tatctccgcg ccgcgcggga gatgcggtaa ggacaggcca ccggcttcgg tcgagcggag
     4741 tgctccgttc cgtatctcga cagttagccg gtgaggcctt tatgcgaggg cgaaactgag
     4801 gcgcatatat atgccgaaaa acgggccgga gggccaaaag aaaaagcgcg atttcttctt
     4861 gacaagcaca ttgcgcgcct ttactctgaa gtcgaacagg gcggatcaaa cgttacaagc
     4921 tccgtccaaa aggttacggg aaggaggtga atagacaatg accatcaccg tcactcgcga
     4981 gaaggtaaag gaagtcacgc gcgaagatgt ggaccgggcc atcgacgtca tcgagcgtca
     5041 tctggagaaa gaggccagga cccgcaacct gctggacgag caggcgatca tgcgtccgaa
     5101 ggacctgtgc aagcagcagg gactggacat ggttgccgag cttcgcggca tgatgtaggc
     5161 cgtgtgaggt acatgcggct tcgggcctat gaagggggat gcacagtaaa cgtccctggc
     5221 tcatccagag tagagggctg tccatccccc ttcacatagg ccgaaaagga ccgggagaga
     5281 tcccggtcct ttttcgtgcg tgcgaatcgc ggatggcgac gatatcaaag ccgtgtactg
     5341 acgcaccgat gtacatgggt cagtcgcaag cgtttctgaa taatcaggac aatatgtccc
     5401 ccgagtttgt gacacctgcc tcgttacggt gggacaccct gcgccgtagg ctgcgctcaa
     5461 gcctcaaggg tgaggtcaaa gagaaggagg ttctcaaatg cctgaacgac acactaactg
     5521 gatccgcata gggctattcg ccctgcccat ctccggggcg ctgctcgcct ggtccagcct
     5581 cgacccccag cccaaccaga acaagaaccc agaggcctgg gctcgcttcg tcggctccac
     5641 ctactacctg cttacccacg tgtttgggtc cgtggggagc gcgctcctcg cgatcttcgg
     5701 cgtgatggcc ctcggtgcct atcttgccaa aagccgcgcc ggtcgcctgg gattggtagc
     5761 gatggtcgtg acggtattgg gtcaggcctt gagcctggtg atcggcgggg tctccacctt
     5821 cgccctgaac gccatcgggc gggcctacct gtcgggcaca aaggacgtca tgcaactgga
     5881 attttcctcc gcgatgtcgg tgctcttcgc gctggctatc ctgctcctga ttgttggcaa
     5941 cgtgctcctg ggtgtggccg tgtggcggtc gggggcattg ccaaagtgga caggaaccat
     6001 ctgggtggct tcggccctga tgttctacgt gctgggtgcc gtgctcggga tggctactac
     6061 cggtagcagc ctgccgacgc agcccgtggg ggggttgctg atggtgataa gcggggtctg
     6121 gatagcctgg agcgccttgc ggcaaccctc cgtcgggata gtgggcgcgg ccgcccaacc
     6181 agggatgcaa taaatgaccc gtcaggaggc gagcctttga ttcacccgca acgcgcttca
     6241 agcagccgag aaaactctcg aaggaggtag atcatgtcca ccgcaaccgc cgcgccgcag
     6301 ccggccccag cctccccgat tcggagactg ctgatccagc atcctctggt gtccttcttc
     6361 gctctggcct acgccctcac gtggctcgcg tggagcccgt ggtatctctc cgaagacggc
     6421 ataggctttc tgcactttga cggggagagc gtatccgact acatcaacac cgtcgccctg
     6481 atagtgggac cgacgctctc ggcgttcatc gtgacgggcg ccaccgaggg gagggagggc
     6541 gtgagacgcc tgctgcgccg catcgtgctg tggcgggtga gtctccagtg gtacctcatc
     6601 gtcctcgtcg gcatccccgc gctcatagtg ttctgcgcga tcgtcatgcc gggggcactt
     6661 gcctcgttta aggcatcggc cgtgccgtcc accctgttcc tgtacgtggt ggctggagcc
     6721 gtcttcctct tcgccggagg gccggtgttc gaggagatag ggtggcgcgg cttcgcgctg
     6781 cccaggctgc aacggctata cggaccgctg gtcgggagcc tcatcctcgg ggttctctgg
     6841 ggtatgtggc acctgccgtt gttcttcatc ccgagttggg acacaccgca cggcagcccc
     6901 ctcgacatcg ccctgttcgt catctgggca gtgtcgatag ccattctctt cacgtgggt
//
LOCUS       DASSMB010000122        14520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_407277, whole genome shotgun sequence.
ACCESSION   DASSMB010000122 DASSMB010000000
VERSION     DASSMB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14520
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407277"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VFJ72_07555"
     CDS             <1..486
                     /locus_tag="VFJ72_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7479356.1"
                     /translation="VLQQVTHGLYSALLFILVGVISSRTGTRRIRELGGLAARMPWAG
                     GLLALGALTAMGLPGLAVFVSEFMSIMGGYTAFPVQGVIAAVGIILSAMYLLYMLART
                     IFGPIDRPAYEEIIDARPIEMAAVVPLAVLIVLLGIFPSLLIAIQRPAVEAVLSAIGG
                     S"
     gene            486..1922
                     /locus_tag="VFJ72_07560"
     CDS             486..1922
                     /locus_tag="VFJ72_07560"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HET7479357.1"
                     /translation="MAVGGDTFIGLLPELIATAFVLIVLMVGVFAQRSVGLVTGLAAL
                     GALAVFGSAVGLLVAGFSGSFFGDAFVVDGFGLYFKLIVAGSAFFAILAAARWSGGTG
                     DAPEYMTLILSVSLGAMLLVSMRDLFGIFLALELATIPSYAMVAFDRSRRESAEGGMK
                     YLLTGVIASSVLLYGIVLIYGVSGSAELSAVSGTFSGRLSPVALLGLVLLLSGFAFKV
                     SAAPFHFWTPDAYQGAPTSAAAFLSVAPKAATFGVLLRILLEGMKEAAPTWTAVMAFL
                     AILTMFVGNLAALRQSNVRRMMAYSSVAHSGYILAGFAALQGAAVQRGVEAVLIYTAA
                     YAVMNMGAFLVIDLVGEDGKSFNGLFRTRPAVALCMGVFMAALVGIPPFSGFWGKAWI
                     ILAGAQSGSILVYVVVGALVINSVLSVPYYFGIIRNMFFEEPTRDALEPRGTGAVKFS
                     VYVLAVATTLFFLLIIPLSALVGASGLL"
     gene            1935..3038
                     /locus_tag="VFJ72_07565"
     CDS             1935..3038
                     /locus_tag="VFJ72_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET7479358.1"
                     /translation="MRAVILVGGQGTRLRPITYDIPKALVPLRNHPFMGYMVDFLRSG
                     GIGGAVLSLGYLPDPIQSYFASRNDLDGFEVGYAVEDHALGTAGGIKNAENYLDGGTF
                     VVVNGDVLTGMELSRAIERHRATDALATITLTSVEDPTAYGLVEVDHEMLVHRFIEKP
                     AYDEVTTNLINAGIYVLEPEVLDMIPAGREVSIEREVFPQLQAEGRLRAHVSSSYWRD
                     IGTPRSYLAASHDVLSGAVGAGADFEYLNVHPSTRVGKNVKLLPPVSVADGCDIGAGA
                     TIGGRSALGRGCRIGEGAVVEGSILLDGAEVEAGAVVRGSILGPRARVGQKAIVRGLS
                     VLGAGCVVGEGNVLDQGIRINPGVVLPPRSIRF"
     gene            3204..3887
                     /locus_tag="VFJ72_07570"
     CDS             3204..3887
                     /locus_tag="VFJ72_07570"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET7479359.1"
                     /translation="MQPYVSALADLFYPQRCVGCESRASDVLCRACYEALPVIEPPVC
                     RRCGMPTAFETFVCDECKNADYGFESARAPLRYEGVGKEIVHALKYRGYTRVVERLMA
                     PLMLGALEAHSGFDAVVPVPLHRSRLRRRGFNQAGLIARAIAAELEATLSDTLEVVRR
                     TRDQVELSGAGRRANVAGAFRARGRVSGRILLIDDVFTTGATMSECAGVLVKAGAEEV
                     RALSLCRTC"
     gene            3923..4483
                     /gene="raiA"
                     /locus_tag="VFJ72_07575"
     CDS             3923..4483
                     /gene="raiA"
                     /locus_tag="VFJ72_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET7479360.1"
                     /translation="MEILVKGRNISVTEALEQYATEKVEKLSKFFDEQHSASRVEVEL
                     IHERNRSNADPEVVETTLFINGTVLKARECSPDMYASIDGMSDKLERQVRRYRGRQID
                     RWQGQKKTETPEPEPFVVEEEEDIETRIVRTKQFQMKPMSAEEAALQLELLDHAFYVF
                     TSADTGDINVVYRRRDGNYGLIEPAR"
     gene            4525..7131
                     /gene="secA"
                     /locus_tag="VFJ72_07580"
     CDS             4525..7131
                     /gene="secA"
                     /locus_tag="VFJ72_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7479361.1"
                     /translation="MANLLTKILRMGEGRKVKALERRVAEVNALEPEIEKLSDSALAA
                     KTDEFRERLAQGATLDGILPEAFAVVREASRRTLGMRPFDEQVMGGIVLHEGKIAEMK
                     TGEGKTLAATMPVYLNALAGKGVHVVTVNDYLVRRDAGWMGQIYQFLGLSVGIIQDGH
                     GFDERKVAYASDITYGTNAQFGFDYLRDNIATETDQLVQRDLVYAIVDEVDSILIDEA
                     RTPLIISGMPESAADTYYRFAAIIPRLKEGEDYEVDEKKRQVAPTESGVEKVEKALGL
                     ENLYDNVNTNLVNHLNQALRAQTLFNRDVEYLVRDGQVFIVDEFTGRVLEGRRYSEGL
                     HQAIEAKEGVEILEENQTVATVTIQNFFRQYDKLAGMTGTAATEADEFMHIYKMEVVS
                     IPTHKDMIRDDRDDLVYKTKKAKYAAAIEDIEERYRKGQPVLVGTVSVEVSEHLATML
                     KRRGVPHNVLNAKQHEREAQTIAEAGERGAVTIATNMAGRGTDIKLGPGVAELGGLYV
                     LGTERHESRRIDNQLRGRSGRQGDPGESRFFLSFEDDLLRLFGGDRMQGIIDRIGLEE
                     DVPIEAGMISNSVRRAQEQVESRNFEIRKRILEYDDVLNKQREVIYAIRRDILMGETV
                     DTWEYVADVLSGVVGEHASDAVYPEDWDLDTLAAELNRFYPCNVDFSRLDVEGMTGED
                     IREMVLKDARERLEDRKVEWDERMAEIEKRGLVRTDNVNTFEEAERRTLLSVVDSRWR
                     EHLMEMDYLREGIGWRGLSQRDPLVEYKREGFDIFQEMERGLKEDYVTYIYRIENVKL
                     QEEPVQQYSYSGGTEEPNPQPKSPRAGQQKVGRNDPCPCGSGKKYKKCGMLNTPEHQR
                     LMAQGGSEAQAG"
     gene            7131..8222
                     /gene="prfB"
                     /locus_tag="VFJ72_07585"
     CDS             7131..8222
                     /gene="prfB"
                     /locus_tag="VFJ72_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET7479362.1"
                     /translation="MTELQEKAAELEERLAELEAFFDVEDLRGRAAKLTEEMSRPGFW
                     DDPDKAKEVSARFSRVEGRLRLLEDLRGRLADSGELMELAEEDGDLLSEVEEELRRVE
                     GVLEEQEVARLFAGEYDEGDAIVTINSGAGGVDSQDWAEMLARMYRRWAERRGFSLEV
                     IEYTEGEEAGIKSATFNIGGEFAFGLLSAERGVHRLVRISPFDAGARRHTSFASVMVA
                     PVIGDAVEVEIEDKDLKVDTYRASGAGGQHVNKTDSAVRITHLPTGIVAQCQNERSQH
                     QNREVAMRVLRARLFELEREKREREIAAQSGEKADIEWGSQIRSYVLHPYKLVKDHRT
                     SEETANTDRVLDGDLDAFIYAFLKSRAVA"
     gene            8232..8960
                     /locus_tag="VFJ72_07590"
     CDS             8232..8960
                     /locus_tag="VFJ72_07590"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HET7479363.1"
                     /translation="MRLLEVLLAAALLIGGAALVGCYTFLPSLLEGMVAGNLQRSMGL
                     ASEPGVKLASDPPPRMLAGEFSRGEIEMKGADLGGVRPDRITVDLDPFDVEVLKSAAS
                     GSLKVREPLTGKMRVELTEQQVSEIARERVEDFPIEGVSLSRDRAVVSSKVEILGFPV
                     PVSVDGEVSARGGALVFEPREVRALGVPVPKRLTDALLAGTDFTYRIEGLPYDTEIKD
                     VRVEEGRAILTGYVRGITLGDSGG"
     gene            9159..9845
                     /gene="ftsE"
                     /locus_tag="VFJ72_07595"
     CDS             9159..9845
                     /gene="ftsE"
                     /locus_tag="VFJ72_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET7479364.1"
                     /translation="MIRFSNVTKVYGNDTVALERVNLEIDPGEFVFLVGASGSGKSTI
                     VRLILKEMEPSAGAIYVNGTRLSSVPRRSIPRHRRNIGCVFQDFKLLPNKTAAENVAY
                     AMEVTGQRRRAIRTKVPQILDLVGLAEKTGKYPDQLSGGEQQRVSIARAFVSQPPILI
                     ADEPTGNLDPDTSVGIMQLLHRINRIGTTVVVATHDREMVDVMRKRVVALDEGRIVRD
                     KVKGAYADEV"
     gene            9835..10719
                     /gene="ftsX"
                     /locus_tag="VFJ72_07600"
     CDS             9835..10719
                     /gene="ftsX"
                     /locus_tag="VFJ72_07600"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HET7479365.1"
                     /translation="MRFNVGFFLREAFKNIRLNLLMSVTAVTTTFICILVLGAGLLVS
                     AHVEGLISSVREDVAVTAFFSPNEKDQKIQEVRKKVEAYPEVSSVKFVSKAEALRNFK
                     QTFADQPEIYEDMGSDVLPASLELQLKDPSKADAVASKLDDEGFAKKDLSYPQQTINR
                     LNEVTGYMIWGLYGATALFLVSSILLISNAIRISIFARRKEIEVMKLVGASDGFVRTP
                     FVFEGLVEGLIGASLAALTVIWLNYLFVDWSRNALPFVPISNQGIDTLLILGVLVAVG
                     VAIGMVGSFLSVTRFLRV"
     gene            10826..12445
                     /locus_tag="VFJ72_07605"
     CDS             10826..12445
                     /locus_tag="VFJ72_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET7479366.1"
                     /translation="MILPAILVRRGKYSVADPLFVEERRPVLVARKLRRGAEAGDIVL
                     VNVREKQRSLRGEVTKVLGTSKDPRNVYDALFASIGTSRDFSEKVEEEAEEVAGRPPD
                     MVDREDLRQLATVTIDGDDAKDFDDAVSIERTDDGYRLRVHIADVTYYLDPGGALDRQ
                     AAFRANSVYLPGTVAPMLPKRLSADVCSLRPDEDRAAVTVEMELNADGSSRSARAFRS
                     LIRSDARLTYDGVDAFLRGERDVEQPELVKAAFELSRRLKTRAAVRGKLELGGREPEY
                     EIGPDGIPVSATVRRSTPARALIEELMVLANEAVAKMLRGRRGGVFRVHEKPDAESLE
                     MLGVRLAAIRMQVEPTPENLGTIAATAKSDAVNYLILRSIPRAFYAPSSPGHYGLALE
                     DYTHFTSPIRRYADVLVHRALLGDDTPPVMAEIATHISEREYRSVVCERTADQYTLMW
                     LMRDRVGDVLEGTVVSTTSFGLFVELETGATGLVHVSKLPGWWELEPNGVVLSNDAIG
                     ASYKVGDQLMVELLDVRPLMQRAELRVVKHL"
     gene            12442..12894
                     /gene="smpB"
                     /locus_tag="VFJ72_07610"
     CDS             12442..12894
                     /gene="smpB"
                     /locus_tag="VFJ72_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET7479367.1"
                     /translation="MSDFARNKKALHDFAIEETYEAGIVLTGPEVKSIREGRANLKES
                     YARVRDGEIYLIGAHISPYENARQGEQLPTRDRKLLLHRKEIDRLAGKIAVEGKTIIP
                     LKLYSRHGNIKLELAVASRKKQYDKRRDIAKKTADREMQRAMKQRLRG"
     gene            12976..13329
                     /gene="ssrA"
                     /locus_tag="VFJ72_07615"
     tmRNA           12976..13329
                     /gene="ssrA"
                     /locus_tag="VFJ72_07615"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            13417..14229
                     /gene="murI"
                     /locus_tag="VFJ72_07620"
     CDS             13417..14229
                     /gene="murI"
                     /locus_tag="VFJ72_07620"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET7479368.1"
                     /translation="MSDPRPIGVFDSGVGGLTVLSEIRDRLPYENTIYFGDTARVPYG
                     PRDLTEVRWFAFEIVRYLVSLDVKLVVIACNTAAAAALRSLQRSFDVPIIGVVEPGAR
                     AAVVETRNRRVGVLATEGTVRSRAYEKAVHSLDAGIDVYAQAAPEFVPLIERGITEGP
                     ELETHARKYLEPLKRYSVDAVILGCTHYPLISETINRIMGPEVRLISSAGQTALEVGR
                     VLSRRGYLRRPRDAEDVGTATYVCSADPDEFAALGSRFLDEEIAAVAPAAAV"
     gene            14267..14340
                     /locus_tag="VFJ72_07625"
     tRNA            14267..14340
                     /locus_tag="VFJ72_07625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14301..14303,aa:Pro,seq:tgg)
     gene            14354..14424
                     /locus_tag="VFJ72_07630"
     tRNA            14354..14424
                     /locus_tag="VFJ72_07630"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14386..14388,aa:Gly,seq:tcc)
     gene            14446..14519
                     /locus_tag="VFJ72_07635"
     tRNA            14446..14519
                     /locus_tag="VFJ72_07635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14480..14482,aa:Arg,seq:tct)
BASE COUNT         2612 a         4326 c         4964 g         2618 t
ORIGIN      
        1 gtcttgcagc aggtgacgca cgggctgtat tcggcgctct tgttcatcct ggtcggcgtg
       61 atctcctcgc gcaccgggac gcggcgcatt cgggagcttg gcgggctcgc cgcccggatg
      121 ccgtgggccg gggggctgct cgctctcggg gctctgaccg cgatggggtt gccggggctc
      181 gcggtcttcg tctcggagtt catgagcatc atgggcggtt acacggcgtt cccggtgcag
      241 ggcgtgatcg cggccgtcgg catcatcctc tcggcgatgt acctgctgta tatgctggcg
      301 cgcacgatct tcggtcccat agatcgtccg gcttacgagg agataatcga cgctcgtccc
      361 atcgagatgg cggcggtcgt gccgctggcg gtcttgatcg tgctactcgg catcttcccg
      421 agtctgctga tcgcgataca acgcccggcg gtcgaggctg tgctgtcggc gattggaggt
      481 agctagtggc tgttggcgga gacactttca tcgggctgct gccggagctg atcgctaccg
      541 cgttcgtcct gatcgtattg atggtcggcg tgttcgcgca gcggtcggtc gggctcgtga
      601 cggggctggc tgccctgggg gcgctcgcgg tgttcgggag cgcggtcggc ctgctggtcg
      661 ccgggttctc ggggagcttc ttcggggatg cgttcgtggt ggacggcttc gggctgtact
      721 tcaagctgat cgtggccggc tccgctttct tcgctatcct cgccgccgcg cgctggtcgg
      781 gcggcacggg cgacgcgccg gagtatatga ccttgatcct ctcggtgtcg ctcggcgcga
      841 tgctgctcgt ctccatgcga gacctgtttg gcatcttcct ggcgctggag ctggcgacga
      901 taccgtccta cgctatggtc gccttcgacc ggagccgccg cgagagcgcc gagggcggca
      961 tgaagtacct gttgacaggg gttatagcgt cgtcagtgtt gctgtacggg atcgtcctga
     1021 tctacggcgt ctccggctct gcggagcttt cggccgtctc cggcacgttc tcgggccgtc
     1081 tgtcgcccgt ggcactgctc gggctcgtcc tgctgctctc cggtttcgcc ttcaaggtct
     1141 cggctgcgcc gttccacttc tggacgccgg acgcctatca gggcgcccct acgagcgccg
     1201 ccgccttcct ctccgtagct ccgaaggccg cgacgttcgg ggtgttgctc cggatactcc
     1261 tcgaaggtat gaaagaggcc gcgcctacct ggacggcggt tatggcgttc ctggcgatcc
     1321 tcacgatgtt cgtcgggaac cttgccgcgc tccggcagag caacgtgcgc cggatgatgg
     1381 cttacagctc ggtggcgcac tcggggtaca tcctggcggg gttcgcggcg ttgcaggggg
     1441 cggcggttca gcgtggggtc gaggcggtct tgatctacac ggcggcctac gcggtgatga
     1501 atatgggggc attcctggtg atagacctcg tcggtgagga cggcaagagc ttcaacggat
     1561 tgttccgtac ccggccggcc gtggcgctgt gcatgggtgt gttcatggcg gcgctcgtcg
     1621 ggataccgcc cttctccggg ttctggggga aggcctggat catcctggcc ggtgcgcagt
     1681 ccgggtccat cctggtgtat gtcgtggttg gggcgcttgt gatcaacagc gtcctctcgg
     1741 tcccgtatta ctttggcatc atccgcaata tgttcttcga ggaaccaacg agggacgcgc
     1801 tggagccgcg ggggaccgga gcggtgaagt tctccgtcta tgtcctggcc gtcgcgacga
     1861 cgctgttctt cctgctcatt atcccgctct cggcgctagt cggcgcgtcg ggtctgttgt
     1921 aggctagggt tcctatgcga gcggttattc tagttggcgg acagggcaca cggctgcggc
     1981 cgatcacata cgatatcccg aaggcgctcg tgcctttgcg gaaccatccg tttatgggct
     2041 acatggtgga cttcctgcgc tccggcggta taggaggggc cgtgctctcg ttgggctact
     2101 tgccggaccc gatccaatcc tactttgcca gccgaaacga ccttgacggc ttcgaggtgg
     2161 gttatgccgt cgaggaccac gcgctaggta ccgcgggcgg tataaagaac gccgagaatt
     2221 acctggacgg cggcacgttt gtcgtggtga acggtgacgt gctgacgggc atggaactga
     2281 gccgggccat agagcgccac cgtgcgaccg acgcgctggc gaccataacg ttgactagcg
     2341 tcgaggatcc tacggcctac ggtctcgttg aagtggacca tgagatgctg gtgcaccgct
     2401 ttatcgagaa acccgcctac gacgaagtta cgacgaacct gatcaacgcg ggcatctacg
     2461 tgcttgagcc ggaggtcctc gacatgatac cagcgggacg cgaggtctct atagagcgcg
     2521 aggtcttccc ccagctacag gccgagggac ggcttcgcgc ccacgtctca agctcttact
     2581 ggagggatat cgggactcct cgcagctacc tggcggcttc gcacgacgtc ctctcggggg
     2641 ctgtgggagc cggagcggat ttcgagtacc tgaacgtcca tccttcgacg cgcgttggta
     2701 agaacgtgaa gctcctgcct ccagtctctg tggcggacgg ctgcgatata ggcgccgggg
     2761 ccacgatcgg cggccggtcc gcgctgggcc ggggatgccg catcggagag ggcgcggtgg
     2821 tggagggcag tatcctactc gacggcgccg aggttgaggc cggggccgta gtgcggggtt
     2881 ccatactcgg cccgagggct cgcgtcggtc agaaggccat cgtccgaggt ctctcggtgc
     2941 tcggagccgg atgcgtcgtt ggtgagggca acgtattgga ccagggcatc cgcatcaacc
     3001 ccggcgttgt acttcccccg cggagtatca ggttttagac tgctttcacc ctggccgcgg
     3061 tagaggcggt tggatagaat gaggtctcgg aactctaccc gtgaggtgtt ggtatggatg
     3121 acgtcccgga gaccgctacc gcggaacctc tgccggccat gcccggccac gccgtccctc
     3181 tagcgcgcat ttcggagtag ccattgcagc cttacgtctc ggcgctggcg gacctctttt
     3241 acccgcagcg ctgcgtcgga tgcgagtctc gggcgagcga cgtcctctgc cgcgcctgct
     3301 acgaggctct gcccgtcata gaacctccgg tatgtcgccg gtgcgggatg ccgacggcct
     3361 tcgagacgtt tgtgtgcgac gagtgcaaga acgcagacta cggtttcgag agcgcccgcg
     3421 ctccactgcg ctacgagggg gtagggaaag agatcgtaca cgctctcaag taccggggtt
     3481 acacgcgggt ggtcgagcgg ctgatggcgc cgctcatgct gggcgctctt gaggcgcatt
     3541 ctgggttcga tgctgtggtg ccggtgccgc tgcaccgttc gcgtctcagg cgccggggtt
     3601 tcaaccaggc agggctgata gctcgggcca tcgcggcgga gcttgaagcc actctttccg
     3661 atacactgga agtcgtgcga aggaccaggg accaggtaga gctctccggc gccgggaggc
     3721 gcgccaatgt tgctggtgcg tttcgggcgc gggggcgggt ctcgggcagg atactcttga
     3781 tcgatgacgt atttaccacc ggcgctacca tgagtgagtg cgccggtgtg ctcgtgaaag
     3841 ccggagcaga ggaggtgcgc gcgctcagcc tgtgcagaac gtgttgaaaa tagccgatag
     3901 ccgccgaaca tagggaggcc atatggagat actggttaaa gggcgcaata tatccgtcac
     3961 ggaggctctg gagcagtacg ctaccgagaa ggtcgagaag ctctccaagt tcttcgacga
     4021 gcagcatagc gcctccaggg tcgaggtcga gttgatccac gagcgcaacc gctccaacgc
     4081 cgatcccgaa gtggtggaga cgactctgtt tataaacggt acggtcctga aggcgcggga
     4141 gtgctcgccg gacatgtacg cctccatcga tggtatgtcc gacaagctgg agcgccaggt
     4201 gcggcgctac aggggacggc agatcgaccg ctggcaaggc cagaagaaga ccgagacacc
     4261 cgagcccgag ccgttcgtgg tggaggaaga agaggatatc gagaccagga tagtcaggac
     4321 caagcagttc cagatgaagc ccatgagcgc ggaggaggcg gcgctccagt tggagctcct
     4381 cgaccacgcc ttctacgtct tcaccagcgc cgatactggc gacataaatg tggtataccg
     4441 gcggcgtgat ggaaactacg ggctcataga gcccgcccgg taatcgcggg atacaggacg
     4501 tagcaccgag cgaggtagac ggttttggcg aatttgctga cgaagatact gaggatgggt
     4561 gagggccgga aggtcaaggc cctggagcgg cgcgtggcgg aggtcaatgc tctggagccg
     4621 gagatcgaga agctctccga tagcgcgctc gctgcgaaga cggacgagtt ccgcgagcgg
     4681 ctcgcgcagg gcgcgacgct ggacgggatc ctgccggaag ccttcgccgt cgtgcgggag
     4741 gcatcgaggc gcaccctcgg tatgaggccg ttcgacgagc aggtcatggg cggcatcgtg
     4801 ctgcacgagg gtaagatcgc cgagatgaag accggcgagg gtaaaacgct cgccgcgacc
     4861 atgcccgtct acctcaacgc tctggccggc aagggcgtcc acgtcgtcac ggtcaacgac
     4921 tatctggtcc gccgcgacgc cggctggatg gggcagatct accagttcct cgggctctcg
     4981 gtcggcatca tccaggacgg ccacggcttc gacgagcgga aggtcgccta cgcctccgac
     5041 atcacctacg gtacgaacgc ccagttcggc ttcgactacc tgcgcgacaa catcgccacc
     5101 gagacggacc agctcgtgca gcgcgacctg gtctacgcca tcgtggacga ggttgactcg
     5161 atcctcatag acgaggctcg tacccccctg atcatctccg gcatgcccga gagcgccgcg
     5221 gatacctact atcgcttcgc cgccatcatc ccgcgcctca aggagggcga ggactatgag
     5281 gtggatgaga agaagcgcca ggtcgccccg accgagagcg gcgtggagaa ggtggagaag
     5341 gccctcggac tcgagaacct ctacgacaac gtgaacacaa acctcgtcaa ccacctgaac
     5401 caggcgctcc gggcgcagac gctcttcaac agagacgtgg agtacctggt gcgcgacggt
     5461 caggtcttca tcgtggacga gttcacgggc cgcgtgctgg aagggaggcg ttactccgag
     5521 ggacttcatc aggccataga ggccaaagag ggtgtcgaga tcctggagga gaaccagacc
     5581 gtcgcgacgg tgacgatcca gaacttcttc cgccagtacg acaagctcgc cggtatgacg
     5641 ggtactgccg ccaccgaggc cgacgagttc atgcacatct acaagatgga ggtcgtctcc
     5701 atcccgacgc acaaggatat gatccgggac gaccgcgacg acctggtcta caagacgaag
     5761 aaggccaagt acgcagccgc catagaagat atcgaggagc gctaccgcaa gggacagccg
     5821 gtcctcgtgg gcacggtctc ggtcgaggtc tcggagcatt tggcgacgat gctcaagcgg
     5881 cgcggcgtcc cgcacaacgt cctgaacgcc aagcagcacg aacgagaggc tcagacgatc
     5941 gcggaggctg gcgagcgggg cgcggtgacg atagcgacca acatggccgg acgaggcacg
     6001 gatatcaagc tcgggccggg cgtagcggag ctgggcgggc tctacgtgct tgggaccgag
     6061 cggcacgagt cgcgccgcat agacaaccag cttcgcggac gtagcgggcg tcagggagac
     6121 cccggcgagt ccaggttctt cctgtctttc gaggacgacc tgctgcggct cttcgggggc
     6181 gaccggatgc aggggatcat agacaggatc gggcttgagg aagacgtgcc tatcgaggcg
     6241 ggtatgatct ccaactccgt ccggcgggcg caggagcagg tcgagagccg caactttgag
     6301 atccggaagc gcatcctgga gtacgacgac gtgctcaaca agcagcgcga ggtgatctac
     6361 gcgatccggc gcgacatcct catgggtgag accgtggaca cctgggagta cgtcgcggac
     6421 gtcctctccg gcgtggtcgg cgagcacgcc tcggacgcgg tctacccgga agactgggac
     6481 ctcgataccc tcgcggccga gcttaaccgc ttctatccct gcaatgtgga cttctcccgg
     6541 ctcgacgttg aaggaatgac gggcgaggac atccgcgaga tggtcctgaa ggacgcccgc
     6601 gagcgcttgg aggaccggaa ggtcgagtgg gacgagcgga tggcggagat cgagaagcgc
     6661 ggcctggtgc gcaccgataa cgtgaacacc ttcgaggagg ccgagcgccg gaccctgctc
     6721 tcggtcgtcg actcccgctg gcgcgagcac ctgatggaga tggactacct gcgcgagggc
     6781 atcggctggc gcggcctctc ccagcgcgat cctttggtcg agtacaagcg cgaggggttc
     6841 gacatcttcc aggagatgga gcggggcctc aaggaagact acgtcaccta catctaccgc
     6901 atcgagaacg tgaagctgca ggaggagccg gtacagcagt actcctacag cggcggcacc
     6961 gaggagccga accctcagcc caagagcccg cgcgccgggc agcagaaggt gggacggaac
     7021 gacccgtgtc cgtgcggctc gggcaagaag tacaagaagt gcggcatgct gaacactccc
     7081 gaacaccagc ggctgatggc ccagggtggc tcggaggctc aggcgggtta gtgacggagc
     7141 ttcaagagaa agccgccgag ttggaagaac gcctcgccga acttgaggcg ttcttcgacg
     7201 tggaagacct ccgggggcgg gcggcgaagc tgaccgagga gatgagccgg cccggtttct
     7261 gggacgaccc ggataaggcc aaggaggtct cggcgcggtt ctcgcgggtg gagggccggt
     7321 taaggctcct cgaagacctt cgtggccggc tcgctgactc cggcgagctg atggagttag
     7381 ccgaggagga cggcgacctg ctctccgagg ttgaggaaga gcttcggcgg gtggagggcg
     7441 tgctggagga gcaggaggtg gcccggctct tcgccggtga gtacgacgag ggcgatgcga
     7501 tcgtcaccat aaactccggc gcgggcgggg tggactcgca ggactgggcg gagatgttgg
     7561 cgcggatgta ccgtcggtgg gcggagcgtc gggggttcag cctggaggtc atcgagtaca
     7621 ccgagggcga ggaggcgggc ataaagagcg ccaccttcaa catcgggggc gagtttgcgt
     7681 tcgggttatt gtcggcggag cggggggttc accggctggt caggatcagc ccgttcgacg
     7741 ccggagcgcg gaggcatacg agcttcgcct cagtgatggt ggcgcctgtg atcggtgacg
     7801 cggtcgaggt cgagatcgag gacaaggacc tcaaggtgga cacgtaccgg gcttccgggg
     7861 ctggtggtca gcacgtcaac aagacggact cggcggtcag gatcacgcac ctcccgaccg
     7921 ggatcgtggc tcagtgccag aacgagagga gccagcatca gaaccgggag gtcgccatgc
     7981 gcgtgctgcg ggcgcggctc ttcgagctgg agcgcgagaa acgagagcgg gagatagccg
     8041 cgcagagcgg cgagaaggcg gacatcgagt gggggtccca gatccgctcc tacgtcctgc
     8101 acccgtacaa gctggtgaag gaccaccgca ccagcgagga gaccgccaac acggaccgcg
     8161 tcctcgacgg tgacctcgac gcctttatct acgctttcct caagagccgg gccgtggcgt
     8221 aggtctggcg cttgaggtta ctggaagttc tgttagcggc ggccctgctg atcggagggg
     8281 cggccctcgt cggttgctac acctttctac cctcgctgct cgaaggaatg gtcgcgggca
     8341 acctgcaacg gagtatgggg ctggcatcag agccgggtgt gaagctcgcg agcgacccgc
     8401 cgccgaggat gctcgccggg gagttctcgc ggggtgagat cgagatgaag ggcgcggatt
     8461 taggcggtgt ccggcccgat aggatcacgg tggacctcga tccgttcgat gtcgaagtgc
     8521 tgaagagcgc cgccagcggg tccctgaaag tccgtgagcc gctcaccggc aagatgcgcg
     8581 tagagctaac agagcagcag gtctcggaga tcgcccgcga gagggtagaa gactttccga
     8641 tcgagggcgt aagcctctcc agggaccggg ccgtcgtgtc gtcgaaggta gagatcctcg
     8701 gttttccggt cccggtctcg gtggatggag aggtatctgc ccgcggcggg gctctggttt
     8761 tcgagccgcg ggaggttcgc gcgctcgggg tgccggttcc gaagcgtctg accgacgcgc
     8821 tgctcgccgg cacggacttc acttaccgta tcgagggatt gccttatgac acggagataa
     8881 aggacgtacg ggtcgaggaa gggcgcgcga tcctgacggg ctatgtccgg ggcattacgc
     8941 ttggtgattc gggtggttga taacgtgctc tattgctgct atcgtctccg atggccggcg
     9001 ggttctcagg aggggggagc ccgccactag agccggaaga tccctcgtgt tgagaggcca
     9061 taaccgtgtg gcgggtgagc gtccccggcg gcggtaatat tccgcggcga gcgcgggcga
     9121 ctggagggac ggtccgccgc agccgacttg gagaggtaat gatccgattc agcaacgtca
     9181 ccaaggtcta cggcaacgac accgttgcgc tagagcgggt gaaccttgag atagaccccg
     9241 gcgagttcgt attcctcgtc ggagcgagcg gctcgggcaa gtccacgata gtgcgcctga
     9301 tcctgaagga gatggaacct tccgcgggcg ccatctacgt gaacgggacc cggctctcgt
     9361 ccgtgccgcg gcgctccatt ccgcgccacc gccgcaacat cggctgcgtt ttccaggact
     9421 tcaagctcct gccgaacaag acggccgccg agaacgtcgc ctacgcgatg gaggttacgg
     9481 gccagcggcg gcgcgccatc cgcacgaaag taccgcagat attggacctc gtggggctcg
     9541 cggagaagac gggcaagtac ccggatcagc tctccggcgg ggagcagcag cgggtctcga
     9601 tagcgcgggc gttcgtcagc cagccgccga tactcatcgc cgacgagccg accgggaacc
     9661 tcgacccgga cacgagcgtc ggcatcatgc agcttctgca caggatcaac cgcatcggga
     9721 ccacggtcgt ggtcgccacg cacgaccgtg agatggtgga cgtgatgcgc aagcgggtcg
     9781 tcgccctcga tgagggccgg atcgtccgcg acaaggtaaa gggggcttac gcggatgagg
     9841 tttaacgtcg gtttcttcct gagagaggca ttcaagaaca ttcgcctgaa cctgctgatg
     9901 agcgtcactg cggtaaccac gacttttatc tgcatcctgg ttcttggcgc cggtctgctg
     9961 gttagcgcgc acgtcgaggg gctcataagc tccgtgcgcg aggacgtggc ggttacagct
    10021 ttcttctctc caaacgagaa agaccagaag atccaggagg tccgcaagaa ggtcgaggcg
    10081 tacccggagg tatccagcgt caagttcgtc tccaaggccg aggccctgag gaacttcaag
    10141 cagaccttcg cagaccaacc ggagatctac gaggacatgg gctcggacgt gcttccggcc
    10201 tccctggagc ttcaactcaa ggacccatcg aaagcggacg cggtagcgtc gaagctggac
    10261 gacgaagggt tcgccaagaa ggacctcagc tacccgcagc agaccatcaa tcgcctcaac
    10321 gaggttacgg ggtacatgat ctggggcctc tacggggcga cggctctctt cctcgtgtct
    10381 agcatcctgc tcatctcaaa cgccatccgc atctcaatct tcgcccggcg caaggagatc
    10441 gaggtgatga agctcgtcgg agcgtcggac ggcttcgtgc ggacgccgtt tgtcttcgag
    10501 gggctcgtgg aggggctcat cggggcctcg ctggccgccc ttaccgtgat ctggctgaac
    10561 tacctgttcg tggactggtc gcgtaacgcc ttgccgttcg tgccgatctc gaaccagggt
    10621 atcgacacgc tcctgatcct gggcgtgctc gtggccgtgg gcgtcgccat cgggatggtc
    10681 ggcagcttcc tctcggtgac gcgcttcctg cgggtgtagc cggcgcagcc ggtggcgggc
    10741 ttcggctctt cactaccggc tgatggtccc tgtcatggta gactcaggcc gtgtttcgcg
    10801 ctgatgcaca tccccggaac gtgtcgtgat actaccggcc atactcgtac ggcgcggtaa
    10861 gtattccgtc gccgacccgc tcttcgtcga agagcggcgt cccgtgctgg tcgcccgcaa
    10921 gctgcggcgc ggcgcggagg ccggggacat cgtcctcgtc aacgtccgtg agaagcagcg
    10981 ctcgctgcgc ggcgaggtca cgaaggtcct cggcacctcc aaagaccctc gcaacgtcta
    11041 cgacgcgctc ttcgcttcca tcggcacctc ccgagatttc tctgagaagg tagaggaaga
    11101 ggccgaggag gtcgctggcc gcccgcccga catggtggac cgcgaagacc tgcgacagct
    11161 cgctacggta acaatagacg gcgacgacgc taaagacttt gacgacgccg tctccatcga
    11221 acgcacagac gacggctacc ggctccgggt ccacatcgcc gacgttacct attaccttga
    11281 cccaggcggc gctctcgacc ggcaggctgc cttccgggcc aactcggtct acctgccggg
    11341 cacggtcgcg ccgatgctcc cgaagcggct ctccgcggac gtgtgctcgc tgcggccgga
    11401 cgaggatcgc gccgccgtta cggtagagat ggagttgaac gcggacggct cgtcacgttc
    11461 ggctcgcgct ttccgaagtc tcatccgcag cgacgcgcgg ctcacctacg acggtgtgga
    11521 cgctttcctg cgcggcgagc gcgatgtgga gcagccagag ttggtaaagg cggcatttga
    11581 actctcgcga aggctcaaga cgcgggcggc cgtgcgcggc aagctggagc tcggcggacg
    11641 cgagccggag tacgagatcg gcccggacgg catcccggtc tcggcgacgg tccgccgctc
    11701 caccccggcg cgcgccctga tcgaggagct gatggtactc gccaacgaag cggtggcgaa
    11761 gatgctgcgc ggcaggcgcg gtggcgtctt ccgggtccac gaaaagccgg atgcggagtc
    11821 gctggagatg ctcggcgtgc ggctcgcggc tatcaggatg caggtcgagc cgacgccgga
    11881 gaacctgggg accatagcgg ccaccgccaa gtccgacgcg gtgaactatc tcatcctgcg
    11941 ctccatccct cgcgctttct acgcgccgtc gagccccggc cactatgggc tcgcgttgga
    12001 agactatacg cacttcacct ccccgatccg gcgctacgcg gacgtcctgg tgcaccgcgc
    12061 cctgctcggt gacgacacac cgcccgtcat ggcggagatc gccacgcata tctccgagag
    12121 ggagtacagg tccgtggtct gtgagcggac cgccgaccag tacaccctga tgtggctgat
    12181 gcgcgaccgt gtcggggacg tgctggaggg gaccgtggtc agcaccactt ccttcgggct
    12241 gttcgtcgag cttgagactg gggcgacggg cctcgtacat gtcagcaaac tccccggatg
    12301 gtgggagctt gagcccaacg gggtagtgct ctccaacgat gccatcggcg cctcttacaa
    12361 ggtcggtgac cagttaatgg tggagttgct cgacgtgcgg cccctcatgc agcgggcgga
    12421 gttgcgggtg gtgaagcatc tgtgagcgac ttcgcgcgca ataagaaagc tctccacgac
    12481 tttgccatcg aggagaccta cgaggccgga attgtcctca ccggcccgga ggtgaagtct
    12541 atccgggagg gacgggcgaa cctgaaggag agttatgcgc gggttcggga tggagagata
    12601 tatctgatcg gggcccacat ctccccttac gagaacgcgc gccaagggga gcaacttccc
    12661 acccgagacc gcaagctact cttgcaccgc aaggagatag accgcctggc cggcaagata
    12721 gcggtggagg gcaagactat catcccgctc aagctctact ccaggcacgg caatatcaag
    12781 ctggagttgg cagtcgccag ccgcaagaag cagtacgaca agcgccgcga catcgccaag
    12841 aagaccgccg accgcgagat gcaacgcgcc atgaaacaac gcctgcgagg ctagagctac
    12901 agttagcggt acggccagag gagcgcgctc ccggctttga aggctgtcct cccggtggta
    12961 gaatatagat cgtaaggggg cgtaccggct tcgacgtgag cggtcagcgt ccagtgagac
    13021 gagccgagtt tgctggagac tcgtaaaacg ccggcaacca agatacacgc cgataacgaa
    13081 atggctctag ctgcttaaat aaaagcggct catcggtccg ggcggcccac ggcccggtca
    13141 accggtgtca tcaagtgggc tcaccttcag ggtcccggtc ccagggctct ttagggacac
    13201 tcaacgggct agccactccg cggcgccccg gtccgcggag accggcgaag agaaaccggg
    13261 agctacgctc gtagaatctc acggacggcg cgctcgcgga cgcgggttcg attcccgccg
    13321 cctccactac aaaggccgtt caatccggac ggccttttct tgttccagat cccgctgaaa
    13381 cgtgaatatc ccggaaaatg gatataatgc tctgccgtga gtgatccacg ccccataggg
    13441 gtcttcgatt ctggtgtggg aggactgacg gttctctccg agattcggga ccgcctgcca
    13501 tacgagaaca caatctactt cggtgacacc gcgagagtgc cctacgggcc gcgcgatctc
    13561 acagaagtgc ggtggttcgc cttcgagata gtgcgctacc tggtaagcct cgatgtgaaa
    13621 ctggtggtca tcgcctgcaa cacggcggcg gcggcggcgc tgcgctcttt gcagaggtcc
    13681 ttcgatgtcc cgatcatcgg tgtcgtcgag ccgggcgcgc gggcggccgt ggtggagacc
    13741 cgaaaccgcc gggtgggcgt cctggctacc gaggggaccg ttcgcagccg cgcctacgag
    13801 aaggccgtgc actcgctgga cgccggcata gatgtctacg cccaggcggc cccggagttc
    13861 gttcccctca tagagcgggg gataaccgag gggccggagc tagagacgca tgcccgaaaa
    13921 tacctggagc cgttgaagcg gtacagcgta gacgcggtga ttctggggtg tacccactac
    13981 cctctaatct ccgagaccat caacaggatc atgggtccgg aggtgcgcct gatctcctcc
    14041 gctggtcaga ccgctctcga agttggccgc gtcctctccc gaagaggcta cctgcgccgg
    14101 ccgcgcgacg cagaggacgt tgggacggcc acctacgtct gcagcgcgga cccggacgaa
    14161 ttcgccgctc ttggcagccg ctttttagac gaggagatag cggctgtcgc tcctgccgcg
    14221 gcggtttaaa ggatcaatgc gtttgttata cttctctgcg gcatcgcggg gcgtggcgca
    14281 gtctggtagc gcacctgctt tgggagcagg gggtcggagg ttcaaatcct ctcgccccga
    14341 cagccagagt catgcgggtg tagctcaacg gtagagcccc agccttccaa gctggttatg
    14401 ggggttcgat tcccctcacc cgctctctac cgccgcgcgt agcgtgcgcc cgtagctcaa
    14461 ctggatagag cagcggactt ctaatccgca ggttgcaggt tcgagtcctg ccgggcgcgc
//
LOCUS       DASSMB010000123         4665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1800102, whole genome shotgun sequence.
ACCESSION   DASSMB010000123 DASSMB010000000
VERSION     DASSMB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1800102"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /gene="pgk"
                     /locus_tag="VFJ72_07640"
     CDS             complement(<1..750)
                     /gene="pgk"
                     /locus_tag="VFJ72_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7479369.1"
                     /translation="MNKRSVRDLDVRGKRVLVRVDFNVPVKDGKVTDDTRIVKALPTI
                     RYLLSEGARPVLISHLGRPKGEPDPKYRMDPVADRLGELLGEPVLKLDEAVGPEVEEE
                     LEDWDGRGVVLLENSRFYPGETKNDPGLSKQLAALAHLYVNDAFGAAHRAHATTVGVA
                     ELLPAAAGLLLEAEVDYLEGLLKDPERPFVAILGGAKVSDKLGVIESLLGVADTLLIG
                     GAMCFTFFKAQGYEIGNSLVEDDYIAEANRLM"
     gene            complement(823..1830)
                     /gene="gap"
                     /locus_tag="VFJ72_07645"
     CDS             complement(823..1830)
                     /gene="gap"
                     /locus_tag="VFJ72_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7479370.1"
                     /translation="MAVRVGINGFGRIGMLTAKAAIERSDDDIEIVAINDLMPMKSLA
                     LLFKHDSTHGIWPEDVSLDDNILRIGDREIKTFAERDPASIPWGDLDVDIVVESSGVF
                     TNKESAGKHLDGGAKKVVISAPAKGVDGTFVFKVNHEEYDPENHAVVSNASCTTNCVV
                     PMAKVLIENFGMESAYMTTCHAYTNDQSLLDAAHKDPRRARAAAQSIIPSSTGAAKTV
                     GVIYPDLKGKVDGMSLRVPISDGSITDLVANLSQEVSVEDINAAFKQAAEGDLAGILE
                     YSEAPLVSKDIVGNPASCIFDAPLTMSGGKTVKVLGWYDNEWGYSNRTVDLVQYMGEK
                     L"
     gene            complement(1956..2738)
                     /gene="whiA"
                     /locus_tag="VFJ72_07650"
     CDS             complement(1956..2738)
                     /gene="whiA"
                     /locus_tag="VFJ72_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET7479371.1"
                     /translation="MDAAGRWEDAGVTVLLSSAAVARSVMRLWRSEFGLESKLSPVAP
                     DRFGRARYAVRTTGNGAMQAADELRFMRSSRNAAERTAYLRGAFQGVGSVTRPGNRGG
                     HHLEFVHQDEEFVRRLAAFSRAPLKISRRRNRWIAYTKSADGVTTLLSQMGLHDAVLE
                     YEARAVLGEAKSNANRVTNFDAANAGRTATAAARQQRALEALDPGKLPSALREMLELR
                     LEHPDASLTELARLGNLTKSAANHRLRRLLEIGDSQSAASGS"
     gene            complement(2824..3786)
                     /locus_tag="VFJ72_07655"
     CDS             complement(2824..3786)
                     /locus_tag="VFJ72_07655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET7479372.1"
                     /translation="MSAAEDLRVVAFGGGTGLPVLLAGLRDRVGDLTAVVTVADDGGS
                     SGRLRQELGVAPPGDVRNCLVALAGRRRLAEVFNYRFEAPGDLNDHSVGNIIIAALSD
                     MAGDFCEGVQQAARFLRIKGQVFPAARESLTLVVRYADGTVTRGESVVRDVGKAVAEV
                     AVEPEGIEAPPGVIEAIEAADVVVLSPGSLFTSTIPALLGAGTREALAEFHGPVVYVA
                     NVMTQRGETMGFTVSDHVRAVARHAGSVITDVLLHSGDLPQDLLARYEAEEAAPVEVD
                     REVLEKMGVRVREADLLSADLRGGVRHDPVKIAEEVCRTALVRL"
     gene            complement(3830..>4665)
                     /locus_tag="VFJ72_07660"
     CDS             complement(3830..>4665)
                     /locus_tag="VFJ72_07660"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7479373.1"
                     /translation="EAGRYHLYIAWACPWAHRTAIMRKLKGLEDAIGLSAVGSFMGDD
                     GWAFYDEPGVIPDTVNGAHYLREVYAKADPEYTGRVTTPVLWDKETGTIVNNESREII
                     RMLDTEFGEFSTTGADYCPDGLRDEIDAIITAIYEPINNGVYRSGFATSQEAYEEAVT
                     ELFDALDYWEGVLSERRYLCGGRITEADWCMFPTLVRFDAVYHGHFKCNLRRIVDYPN
                     LWGYLRDLYQQPGVAETVNMEHIKKHYYRSHESINPTRIVPKGPILDFSGTHGRDRMS
                     G"
BASE COUNT          764 a         1602 c         1459 g          840 t
ORIGIN      
        1 catgagacga ttcgcctctg cgatgtagtc gtcctcgacg agcgagttcc cgatctcgta
       61 cccctgcgcc ttgaagaacg tgaagcacat cgcgccgccg atgaggagcg tatccgccac
      121 gccgagcagg ctctctatta caccgagctt gtccgagacc ttcgccccgc cgaggatagc
      181 gacgaacgga cgctccgggt ccttgagcaa tccctccagg taatccacct ccgcctccag
      241 cagcagccct gcagccgccg gcagcaactc cgcgacgccg accgtcgtgg cgtgcgcccg
      301 gtgcgccgcc ccgaaagcat cattgacgta gaggtgggcg agggcggcga gctgtttgga
      361 gaggcccggg tcgtttttgg tctcgccggg gtagaagcgg ctgttctcca gcagcaccac
      421 gccccggcca tcccagtctt cgagttcttc ttcgacttcc ggtccgacgg cttcatccag
      481 cttgaggacc ggctcgccga gaagctctcc gagacggtcg gcgacggggt ccatcctgta
      541 cttcgggtcc ggctcgccct tggggcgtcc gagatgagag atcaatacgg gccgcgcccc
      601 ctcggagagt agataccgga tggtcggcag cgccttcacg atgcgcgtgt cgtccgtgac
      661 cttcccatcc ttgaccggca cgttgaagtc cacccgcacc agcacccgct tgccccgcac
      721 gtcgaggtct ctaacgctcc gcttgttcat cgtacctccg ggaaagtgag cggtccggga
      781 acgtcctcgc ccccggacct gagagatctc cttgagctgc gcctacagct tctctcccat
      841 gtactgcacc aggtcgacgg tgcggttgga gtagccccac tcgttgtcat accagccgag
      901 caccttgacg gtcttgccgc cggacatggt cagcggcgcg tcgaagatac aggaggccgg
      961 gttgccgacg atgtccttgg agaccagtgg agcctcggag tattcgagaa tacccgcgag
     1021 gtcgccctcg gcggcctgct tgaaggcggc gttgatgtct tcgaccgaca cctcttgcga
     1081 gaggttcgcc acgaggtcgg tgatggagcc gtcggagatt gggacccgca gcgacatccc
     1141 gtcgaccttg cctttgaggt ccgggtagat cacgccgacc gtcttcgcgg cgcccgtgga
     1201 gctcgggatg atggactgag ccgccgcccg cgcccgcctc gggtccttgt gcgccgcgtc
     1261 cagaaggctc tggtcgttgg tgtaggcgtg gcaggtcgtc atgtacgccg actccatgcc
     1321 gaagttctcg atcagcacct tcgccatcgg cacaacgcag ttggtcgtgc aactcgcgtt
     1381 ggagacgaca gcgtggttct cgggatcgta ctcctcgtgg ttgaccttga agacgaacgt
     1441 accgtccacg cctttggccg gagccgagat aacgactttc ttcgccccgc catcgaggtg
     1501 tttgccggcg gattccttgt tcgtgaacac accactcgac tcgaccacta tatccacatc
     1561 gaggtcgccc cacgggatgc tagccgggtc gcgctcggcg aaggtcttta tctcgcggtc
     1621 gccgatgcgt aggatgttat cgtccaggct aacgtcctcc ggccaaatgc cgtgagtcga
     1681 gtcgtgcttg aagagcagcg ccaggctctt catgggcatg agatcgttta tcgccacgat
     1741 ctcgatgtcg tcgtcactgc gctcgatggc cgccttggcg gtcagcatcc cgatccgtcc
     1801 gaagccgtta atccctactc gtacagccac tttttctcct ctcctagctt gctagcgaac
     1861 ccctattgtg cccgaaactc tagagcatta acagagtacc acctgagtcc agccggcacc
     1921 ccccggccca gcgatgtttc gggacgtttg ggggttcagc ttccgcttgc cgctgactgc
     1981 gaatcaccga tctcgagcag ccggcgcaaa cgatggttgg cggcgctctt ggtgagattt
     2041 ccgagccggg ccaactccgt gagcgaagcg tccgggtgtt ccagccgcaa ctccaacatc
     2101 tcgcgcaacg cggacggcag ctttccgggg tccagggctt cgagagcccg ctgctggcgg
     2161 gcggcggcgg ttgcggtgcg tccggcgttg gcggcgtcga agttggtgac gcggttcgcg
     2221 ttcgacttgg cctccccgag gacggcgcgg gcctcgtatt cgaggacggc gtcgtgcagg
     2281 cccatctgag agagcagagt cgtcacgccg tcggcgctct tggtgtaggc gatccagcgg
     2341 ttgcgccgcc gcgatatctt gagcggcgcc cgcgagaacg cggccagacg ccgaacgaac
     2401 tcctcgtctt ggtgaacgaa ctccagatgg tgacccccgc ggttgccggg acgggtgacg
     2461 gagccgacgc cctggaaagc tccgcgaagg taggcggttc gttcggcggc gttgcgggag
     2521 gagcgcatga accttagttc atcggcggcc tgcatcgctc cgtttcctgt ggtgcgcacg
     2581 gcatagcggg cgcggccgaa gcgatccggc gccaccggcg agagcttcga ctccagaccg
     2641 aactccgagc gccacaggcg catcacggag cgggcgacgg cggcgctgga gaggagaacg
     2701 gttacgcccg cgtcctccca tctaccggcc gcgtccacga taccggcaag ctccgcgcgt
     2761 cgggatgggc gcgaaccggt cctggaggtc tcggagcttc cggccgcgag ttgccggcgg
     2821 acctcagagg cgaacgaggg cagtcctgca cacctcctcg gcgatcttga caggatcgtg
     2881 acgcacgccg ccccggaggt ccgcggagag caggtccgcc tcccgcaccc tgacgcccat
     2941 cttctccaga acctcccggt cgacctccac cggagccgcc tcctcggcct cgtaccgggc
     3001 gagcagatcc tggggcagat cgccggagtg gagcagcaca tcggttatga ccgaccccgc
     3061 gtgacgggcg acggcccgga catggtccga gaccgtgaac cccatcgtct cgcccctctg
     3121 ggtcatgacg ttcgccacgt agacgaccgg tccatggaac tccgccagcg cctcccgcgt
     3181 cccggcgccc aacagagccg ggatcgtgct cgtaaacagg cttcccggac tcaggaccac
     3241 cacgtccgcc gcctcgatgg cctcgatgac acccggtgga gcctcgatac cctccggctc
     3301 caccgcgacc tccgcgacag ccttgccaac gtcgcgcacg acggactcgc cccgcgtgac
     3361 cgtcccgtcc gcgtagcgca cgacgagcgt caggctctcc cgggccgccg ggaagacctg
     3421 gccctttatc cgcaggaaac gagcggcctg ctgcacgccc tcgcagaagt ctcccgccat
     3481 gtccgagagc gccgcgatga tgatgttgcc gacgctgtga tcgttcaggt cgccaggcgc
     3541 ctcgaaacgg tagttgaaga cctcggcgag cctccgccgc ccggcgagcg cgaccaggca
     3601 gttcctcaca tcgcccggag gcgcgacccc gagctcctgg cgcaaccgcc cgctcgaccc
     3661 tccatcgtcc gcgaccgtga cgaccgccgt aaggtccccg acccggtccc ttagcccggc
     3721 cagcagcacc ggcaggccgg taccaccgcc gaaagccaca acccgcaagt cttctgccgc
     3781 actcatcagc gccgagtata ccgccgaggc tgccgtaaac gcagcgggcc tagcccgaca
     3841 tccggtcgcg cccgtgagtc ccggagaagt ccaggatcgg gcctttcggg actatgcggg
     3901 tcgggtttat tgactcgtgg ctccggtagt agtgcttctt gatgtgctcc atattgaccg
     3961 tctcggcgac gcccggctgc tggtagaggt cccgcaggta gccccacagg ttcgggtagt
     4021 ccacgatgcg ccgcaggttg cacttgaagt gtccatggta cacggcgtcg aagcggacga
     4081 gcgtggggaa catgcaccag tcggcctcgg tgatcctgcc gccgcacagg tagcgccgct
     4141 ccgagagcac gccctcccag taatccaggg cgtcgaacag ctcggtgacg gcctcctcat
     4201 aggcttcctg gctggttgcg aatccggagc ggtagacgcc gttgtttata ggctcgtaga
     4261 tggcggtgat gatcgcgtcg atctcgtcac gcaggccatc cgggcagtag tcggcgcccg
     4321 tggtcgagaa ctccccgaac tcggtgtcga gcatgcggat gatctcgcgc gactcgttgt
     4381 tcacgatggt gccggtctct ttgtcccata gcacgggcgt cgtaacccgc cccgtgtact
     4441 ccgggtccgc cttcgcgtag acctcgcgca ggtagtgagc cccgttgacc gtatccggga
     4501 tgacgcccgg ctcatcgtaa aaggcccagc cgtcgtctcc catgaacgag ccgaccgccg
     4561 agagcccgat ggcgtcctcc agccccttga gcttcctcat aatggccgtc cggtgcgccc
     4621 acggacaggc ccacgcgatg tacagatggt aacgccccgc ctccg
//
LOCUS       DASSMB010000124         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1674256, whole genome shotgun sequence.
ACCESSION   DASSMB010000124 DASSMB010000000
VERSION     DASSMB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674256"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..506)
                     /locus_tag="VFJ72_07665"
     CDS             complement(219..506)
                     /locus_tag="VFJ72_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479374.1"
                     /translation="MAQTEPNNEAMKQALKEAFAETLHEQRELLHEVFAEVLEDFALA
                     EAIREGQQTERMDRDEVFDESDRLKLFCGARGMWKDRVDLPEFEALRRKFD"
     gene            complement(555..863)
                     /locus_tag="VFJ72_07670"
     CDS             complement(555..863)
                     /locus_tag="VFJ72_07670"
                     /inference="COORDINATES: protein motif:HMM:NF021873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HET7479375.1"
                     /translation="MSSSTTETSVVRAETVTITGDALTVELSDGRSISAPLAWYPRLL
                     HGTPEERKNWRMSGGGVGIHWPALDEDISVRNLLMGKASGESQRSLKRWLEQRDSSSA
                     "
     gene            complement(997..1371)
                     /locus_tag="VFJ72_07675"
     CDS             complement(997..1371)
                     /locus_tag="VFJ72_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479376.1"
                     /translation="MVGGYAVALHGHPRYTKDVDIWVGTDEENAGRLIRAIEDFGFGS
                     LGLKAEDFLVPDQIVQLGYPPSRIDLITTLPGVLFEECYASRVEVHVDGLTIHFIDLE
                     NLKSNKRASGRYQDLADLENLQ"
     gene            complement(1607..>3002)
                     /locus_tag="VFJ72_07680"
     CDS             complement(1607..>3002)
                     /locus_tag="VFJ72_07680"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HET7479377.1"
                     /translation="TAHDTPHRKERDSMGEVEVPRDALFGAQTRRALDNFPISDLRFP
                     RRLIEALGAIKLEAAVVNNELGGLDDHLKDAIVQAAEEVIEGKLDNQFVLDIFQTGSG
                     TSTNMNANEVISNRAIQIAGGELGSKDPVHPNDHVNRGQSSNDVIPTAIHLSALISIK
                     EDLLPGLQKLQSALEDKAREFDDVVKTGRTHLQDATPIRLGQEFQGHAGQIERGILRV
                     NKAVEDLAEVALGGTAVGTGVNTHTEFAGRVCERLSRRFGVEVRETENHFQAQSAMDA
                     AVFASGALKTVAVSLLKIANDIRWLGSGPRASFAEIALPEVQPGSSIMPGKVNPVIAE
                     SAAMVAAQVIGNDATIAVAGQSGNFELNVMLPVIAHNLLQSIEILGSAASNLTDQCVA
                     GLEATDRGPALVEQGLMLATALAPEIGYDKAAEISKDAYKSGKTIREVARENTDLSEG
                     ELDKLLDARKMTER"
BASE COUNT          486 a          934 c          924 g          658 t
ORIGIN      
        1 aacaggtccc ggatcaactg gtacgcacgc tcgatccgct ctcccggcgc cagcacctgc
       61 gcgaaatcat cctcgatctt cgacggctcg atctccgacg cattccccag caacccatct
      121 agcagtccca tcttactctc cctccgtggc taaccgtttg caagctcata aggcacagta
      181 acctttgaga tcaatcgctc tacggcacat ttgctacgct agtcgaactt acgacgcaac
      241 gcctcgaatt cgggcaagtc gacgcgatct ttccacatgc cccgcgcgcc acaaaacaac
      301 tttagccggt cactctcatc gaagacctcg tcgcgatcca tgcgctcggt ctgctggcct
      361 tctcgaatag cctcagcaag agcgaaatct tccagcacct cggcgaagac ctcgtgcagc
      421 agttcccgtt gctcgtgcaa cgtctcggcg aacgcttctt tcaacgcctg cttcatcgct
      481 tcgttgttcg gttcggtctg tgccatctca tcgcctcgtt acgaaggtat ctactcaata
      541 gtagcgcatc gcggtcaggc gctggacgaa tcccgctgct ccagccaccg cttgagcgac
      601 cgctggctct cgccggatgc cttgcccata aggaggttcc gcacgctgat gtcttcatcg
      661 agtgcgggcc agtggatgcc gacgccccca ccactcatcc gccagttctt gcgctcctcg
      721 ggggtgccgt gcaggaggcg cggataccac gcaagaggcg cggagatcga gcgtccgtcg
      781 gagagctcga cggtgagcgc gtcgccggtt atggttacgg tctcggcgcg tacgacggaa
      841 gtttcagtcg ttgaagaact cattccagct ccttagcagt tcctctcggt gttcctcgac
      901 cagtctctgt atgcgtccga tctccgcgct cctcacggaa ctccaggcat tcgtctatcg
      961 agtcccgaaa cgccgtcttc agtcgttcac ggaaagtcat tgcaggttct ccagatccgc
     1021 caagtcctga taccgccccg aagcccgctt gttgctcttg aggttctcca ggtcgatgaa
     1081 gtggatagtt agaccgtcga catggacctc cacacgggaa gcgtaacact cttcgaagag
     1141 aacgcccggc aaggtcgtta tgagatctat gcggctgggt ggatagccga gttgaactat
     1201 ttggtccggc accaggaagt cctcggcttt cagaccgaga gatccgaacc cgaagtcttc
     1261 tatagctcga atcaggcgtc cggcgttctc ttcgtccgtc ccaacccaga tatctacgtc
     1321 cttggtgtac cggggatgcc cgtgcagcgc gaccgcgtag ccgccaacca ctaggtaacg
     1381 gaccctagcc ccgtttaacg aagcgaagaa atctctgaag tcggggctga gtatcataat
     1441 gccactggtg gtactctcgt cggatctcct ccagtgcggc cagccgcgct tcgtagggtt
     1501 gcttccgcca gtaagcaaaa tcggtcggct gctcttcaag ggggaccttc cgcactattt
     1561 tccgcacagg cttgcttccc tccatgattc gcgctccgcc cttattctac ctttctgtca
     1621 tcttgcgcgc gtcgagcagc ttgtccagct ctccctcgga gaggtcggtg ttttcgcggg
     1681 ctacctcgcg gatggtcttg ccggacttgt aggcgtcctt ggagatctcg gctgccttgt
     1741 cgtagccgat ctcgggggcg agcgccgttg cgagcatgag gccctgctcc acgagcgccg
     1801 ggccgcggtc ggtggcttct agacctgcga cgcactggtc ggtcaggttg gaggctgcgc
     1861 tacccaggat ctctatgctc tggagcaggt tgtgggcgat gaccgggagc atgacgttca
     1921 gctcgaagtt gccgctctgt cccgctacgg cgatggtggc gtcgttgccg atgacctgcg
     1981 cggcgaccat cgcggcgctc tcggcgatga cggggttgac cttgccgggc atgatggagg
     2041 aaccgggctg cacctccggc agcgctatct cggcgaagct ggcgcgcggg ccgctcccta
     2101 gccagcggat gtcgttggct atcttgagga ggctgacggc gacggtcttt agcgcgccgc
     2161 tggcgaagac ggcggcgtcc atggcgctct gggcctggaa gtggttctcc gtctcccgga
     2221 cctcgacgcc gaagcgccgg ctcaggcgct cgcagaccct gcccgcgaac tcggtgtggg
     2281 tgttgacgcc ggtgccgacg gcggtgccgc ctaaagccac ttcggcgagg tcttcgaccg
     2341 ccttgttcac gcgcaggata ccgcgttcta tctggccggc gtggccctgg aactcttgcc
     2401 ccaggcggat cggggtggcg tcctgcaggt gcgtgcggcc agtctttacc acgtcgtcga
     2461 actcgcgggc cttgtcttcg agggcggact ggagcttctg caggccgggg aggaggtctt
     2521 cttttatgga gatcaaggcg gagaggtgga tggcggtcgg gatgacgtcg tttgaggact
     2581 ggccccggtt gacgtggtcg ttggggtgga cggggtcctt ggagcccagc tcgccgcccg
     2641 ctatctggat ggcgcggttg gagatcacct cgttcgcgtt catgttggtc gaggtgccgg
     2701 agccggtctg gaagatgtcg aggacgaact ggttgtccag cttaccttcg atgacctcct
     2761 ccgccgcctg cacgatggcg tccttgaggt gatcgtcgag gccgccgagc tcgttgttga
     2821 cgacggcggc ttcgagcttt atcgcgccga gggcttctat caagcgcctg ggaaagcgca
     2881 ggtcgcttat cgggaagttg tcgagggcgc gacgggtctg ggcgccgaag agggcgtcgc
     2941 gggggacttc cacctctccc atcgagtcgc gttccttgcg gtgcggggtg tcgtgcgcgg
     3001 tc
//
LOCUS       DASSMB010000125         3564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_28057, whole genome shotgun sequence.
ACCESSION   DASSMB010000125 DASSMB010000000
VERSION     DASSMB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28057"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..461)
                     /locus_tag="VFJ72_07685"
     CDS             complement(123..461)
                     /locus_tag="VFJ72_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET7479378.1"
                     /translation="MTTTRAVTKMRIPTDRPPTSPGEMLLEEFLKPLGITQVQLAKRI
                     RVPYVRVNELVHGKRRVTPSTALRLAKVFGTSPEFWLNGQLALDLYRTINDKKEIEEL
                     DQIEPITHVR"
     gene            complement(448..741)
                     /locus_tag="VFJ72_07690"
     CDS             complement(448..741)
                     /locus_tag="VFJ72_07690"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7479379.1"
                     /translation="MIASFRDEGTEDVFDGRDTKKARKTCPQNLWRVAHRKLDQLNQA
                     ADLDDLRAPPGNRLETLAGDREGQHSIRINAQYRVCFVWTEAGAEEVEIVDYH"
     gene            complement(781..1050)
                     /locus_tag="VFJ72_07695"
     CDS             complement(781..1050)
                     /locus_tag="VFJ72_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7479380.1"
                     /translation="MAKTHKVTFKKRGVTIDVAEDEYILEAAEDAGLTLPYDCRSGTC
                     TTCIQKCLEGEIDQDMAFAIGDEELEQGLRLICIGSPLSDVVLDA"
     gene            complement(1120..3468)
                     /locus_tag="VFJ72_07700"
     CDS             complement(1120..3468)
                     /locus_tag="VFJ72_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7479381.1"
                     /translation="MYKEVYVPVDNSDYSNQACVIGVDVARKFGGRVAGCHAYAAKMH
                     DVRFRQMESGLPEEFRDEDEMKRQRKIHDQLITKGLEIITDSYIDVLEPLCEKYDVEL
                     VRRSLEGKNFKVIVEDVNQNDFDLVVIGAMGMAAVKDTVLGTVTERVVRRLEKADTLI
                     VKDLDRSPFEHIVVAVDGSAKSLGGLKRAIELAREFGGTVEAISVFDPYFHYAMFHSI
                     AGVLSSKAQKVFRFKEQEKLHEEIIDSGLAKIYTAHLEIAKKIAADEGVELKTTLLAG
                     KPFEQTMKYVNEVNPTLVVMGRIGYHSDDEMDIGGNTENMMRYLPTNVLITNFEYQAP
                     DILTAEEHMTWTKEALERLDRIPGFVVGMATGAIIRYAIERGYTVITTGVIDEVIKNI
                     LPPGAMESMRAIGDQMREAEAMGEDKPIDSLFKDFDERTADKKAAKAKANGNGANGSA
                     NGNGHVTKEEIFEAGRGGFGKSEDQGDIEGVSAEVQAEIHRVAQGHDRFECDVCRYVA
                     KGKPAKCPVCGSSPAHFHAVDAEIATTAGDENLNLSEVYDGRELHWTDDAQRLLDSLE
                     EWQEKRRVRARVEKSALKKGYTTITREYVEQQYREETGREAPEVTQPQAGGCPVSHVK
                     QQVEASTGATCPIDHSAFQKAGALVPEGGSKEFTWTQEAIDRLERAPKGFMRNISRNM
                     TEKLARERGVTHIDLPLVEDSLSGARTTMEDVITGKISIADLAKDTGEKIEADGNGEA
                     PHPPMTVTMVCGTCGEEMQGVQPPEECPVCGAPGEDFEAREL"
BASE COUNT          543 a         1144 c         1088 g          789 t
ORIGIN      
        1 cggcgagcgg ttggccgaac tcatcgactg gaaagccatc attgcgggca cggacgaaaa
       61 gttccgcgtc ggccgcgacc agaagacctt tccccaatac gcacaaccaa cgacgtagag
      121 actcatcgaa cgtgcgtgat cggctctatc tgatccaact cttcgatctc ctttttgtcg
      181 tttatcgtcc ggtagagatc cagcgcgagt tgcccgttta gccagaactc ggggctggtg
      241 ccgaacacct tcgccagccg cagggccgtc gaaggggtaa cgcgccgctt gccgtgaacg
      301 agctcgttca cccggacgta ggggacccgt atccgcttcg cgagctggac ttgagttatg
      361 cccagcggct tcaggaactc ttctagcagc atctcgccgg gagaggtagg gggtctatcc
      421 gtcggtatcc gcatcttcgt caccgcccta gtggtagtca actatctcga cctcctctgc
      481 cccggcctcc gtccagacga aacacacgcg gtactgggcg ttgatcctta tgctgtgctg
      541 accttctctg tctcccgcca gcgtctccag acggttgccg ggtggggctc gcaagtcgtc
      601 cagatccgcg gcctggttga gttggtccag cttcctgtga gccacgcgcc agagattctg
      661 agggcacgtc ttccgcgcct tcttcgtgtc ccgtccgtcg aaaacgtctt ccgtaccctc
      721 gtccctgaac gaagcgatca cgcttgcgca gtataacgca taacgttata cgtgctaaat
      781 tcaggcgtct agcactacgt cggagagggg gcttccgatg cagatgaggc gcaggccctg
      841 ctctagctcc tcgtcgccga tggcgaaggc catgtcctgg tcgatctcgc cttcgaggca
      901 cttctggatg caggtggtgc aggtgccgct gcggcagtcg taggggaggg tgaggccggc
      961 gtcctcggcg gcttcgagaa tgtactcgtc ctcggcgacg tctatggtga cgccgcgctt
     1021 cttgaaggta actttgtggg tcttcgccat gtactctcct aaagttaagg ccccgacccc
     1081 gactcgcggg accggggcct ctgttcggtt acgttgctct tacagctctc gggcctcgaa
     1141 gtcctcgccg ggcgcgccgc agacggggca ctcctcgggg ggctggactc cctgcatttc
     1201 ctcgccgcag gttccgcaga ccatcgtgac ggtcatcggc gggtgcgggg cttcgccgtt
     1261 cccgtcggct tctatcttct cgccggtgtc tttcgcgagg tcggcgatgg agatcttgcc
     1321 ggtaatgacg tcctccatcg tggtgcgcgc cccggagagc gagtcctcca ccagcggaag
     1381 gtctatgtgg gtaacgccgc gctcgcgcgc cagcttctcg gtcatgttgc gcgagatgtt
     1441 ccgcatgaag cccttcggcg cccgctccag gcggtctatg gcctcctgcg tccaggtgaa
     1501 ctccttggag ccgccctcgg ggaccaatgc gccggccttc tggaaggcgg agtggtctat
     1561 gggacacgtg gcgccggtcg acgcctcgac ttgctgcttg acgtgcgaga cggggcagcc
     1621 gccagcctgc ggctgcgtga cctcgggggc ctcgcggccg gtctcctcgc ggtactgctg
     1681 ctcgacgtac tcgcgggtga tggtagtgta gcccttcttt agcgcgctct tctcgacgcg
     1741 ggcgcggacg cggcgcttct cctgccactc ttcgaggctg tcgaggagcc tttgggcgtc
     1801 gtcggtccag tgaagctcgc ggccgtcgta gacctcggag aggttcaggt tctcgtctcc
     1861 ggcggtcgtg gcgatctcgg cgtctacggc gtggaagtgt gccgggctgg atccacagac
     1921 cggacacttc gcgggcttgc ccttcgccac gtagcggcac acgtcgcact cgaagcggtc
     1981 gtggccctgg gctacgcggt ggatctccgc ctgaacctcc gcggagacac cctcgatgtc
     2041 gccctggtcc tcgctcttgc cgaagccgcc gcggcccgcc tcgaagatct cctctttcgt
     2101 cacgtgcccg ttgccgttgg cggaaccgtt cgcgccattg ccgtttgcct tcgccttcgc
     2161 ggccttcttg tcggcggtgc gctcgtcgaa gtccttgaac aggctgtcta tgggcttgtc
     2221 ctcgcccatc gcttcggcct cgcgcatctg gtcgccgatg gcgcgcatgg actccatggc
     2281 gccgggcggg aggatgttct tgatgacctc gtcgatgacg ccggtggtga tgaccgtgta
     2341 gccgcgctcg atggcgtagc ggatgatcgc gccggtcgcc atccccacca cgaatcctgg
     2401 tatgcggtcg agccgctcca gggcctcctt cgtccaggtc atgtgttcct cggcggtgag
     2461 gatgtccggg gcctggtact cgaagttcgt tatgaggacg ttcgtcggca ggtaacgcat
     2521 catgttctcg gtgttgccgc cgatgtccat ctcgtcgtcg gagtggtagc cgatgcggcc
     2581 cataaccacg agcgtcgggt tgacctcgtt cacgtacttc atcgtctgct cgaacggctt
     2641 gcccgcaaga agcgtcgtct tcagctcgac accctcatcg gcggcgatct tcttggcgat
     2701 ctccaggtgc gcggtgtaga tcttggcgag tcccgagtcg atgatctcct cgtgcagctt
     2761 ctcttgctct ttaaagcgga agaccttctg agccttgcta ctgaggacac ccgctatgga
     2821 gtggaacatc gcgtagtgga agtacgggtc gaagacggaa attgcctcga cggtgccgcc
     2881 gaactcccgc gccagctcta tcgcccgctt caggccgccg agcgacttgg ccgagccgtc
     2941 caccgcgacg acgatatgct cgaacggtga acggtcgagg tccttgacga tcagggtgtc
     3001 cgccttctcc agacggcgca cgacgcgctc ggtgacggtg ccgaggaccg tatccttcac
     3061 ggctgccatg cccatcgcgc cgatgacgac gaggtcgaag tcgttctggt ttacgtcctc
     3121 gacgatgacc ttgaagttct tgccttccag ggagcggcgg accagttcga catcgtactt
     3181 ctcgcagagc ggctccagca cgtctatata ggagtcggtg atgatctcca gacctttagt
     3241 tatcaactgg tcgtggatct tgcgctgccg cttcatctcg tcttcgtcgc ggaactcctc
     3301 cgggagaccg ctctccatct gacggaagcg gacgtcgtgc atcttggccg cgtaggcgtg
     3361 acaacccgcg accctgcccc cgaacttgcg cgccacgtca acgcctatga cgcacgcctg
     3421 gttcgagtag tccgagttgt ctaccgggac gtaaacctct ttgtacattt aacccgctcc
     3481 cctagatttc aggacgcctg atccgatacg cacaaaccat tctcgcagaa tacgctccgc
     3541 gcctgtgtaa tgtacctttt accg
//
LOCUS       DASSMB010000126        10630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_660943, whole genome shotgun sequence.
ACCESSION   DASSMB010000126 DASSMB010000000
VERSION     DASSMB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660943"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2190
                     /gene="uvrA"
                     /locus_tag="VFJ72_07705"
     CDS             <1..2190
                     /gene="uvrA"
                     /locus_tag="VFJ72_07705"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7479382.1"
                     /translation="TDSIETALKLAEGLVQIETVETDGPGESMLFSESFTCTNCGASI
                     AEIQPRTFSFNSPHGACDRCDGLGSRLEIDPALVVPNEDLSVNDGAVVPWANSQTEYH
                     GSVLGALSEKYGIDLDAPWRDLPEEQKNLILFGTNGDRIYVSYRNRYNRRRQYMTQFD
                     GVVGNLEKRYSETDSEYRREKIEEFMSHVPCPKCKGARLRPEALAVTVGEKNIAEFTA
                     LSVTDAQEFFGGVEFTAREWLIGERVVKEIRERLGFLVDVGLGYLTLGRSAGTLSGGE
                     AQRIRLASQVGSGLVGVLYVLDEPSIGLHQRDNRRLLTTLEKLRDLGNTLIVVEHDEE
                     TIRAADYVIDVGPGAGVHGGEIIAAGSVEDIEAEERSVTGDFLAGRRRIEPPEERRQP
                     TGSIGLMGAMEHNLKDVDVEFPLGIFTAITGVSGSGKSTLVNEILYKALANAVSRGKH
                     RPGRHAGLRGVEDVDKVIDIDQSPIGRTPRSNPATYTKVFDHIRQLFAATPEAKIRGY
                     KPGRFSFNVKGGRCEACRGDGQIRIEMHFLPDVYVPCEVCKGRRYNTETLRATYKGKS
                     ISDVLDMTIEEACEFFAPVPAIARRMETLRDVGLGYVRLGQPATTLSGGEAQRVKLAS
                     ELGKRATGKTVYILDEPTTGLHFADVEKLLHILHRLVDAGNTVIVIEHNLDVIRSADH
                     VIDLGPEGGDGGGEIVAVGTPEDVARVEESHTGRFLRGLMPEVMAAS"
     gene            complement(2194..2643)
                     /locus_tag="VFJ72_07710"
     CDS             complement(2194..2643)
                     /locus_tag="VFJ72_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479383.1"
                     /translation="MESQDQDKFSELFAVIQDYSRREYDHQDKALQIIAGTYVFMFES
                     EDMPDARHVVDGILEQYDYVFTTLERGNLDPLSVDAVVRVALYSEEYMEWGINRLGRI
                     LEGLFRRSRADGSYTDYVEDASVVIRGLENIVAGSVLEELVEGTNGP"
     gene            2798..3625
                     /locus_tag="VFJ72_07715"
     CDS             2798..3625
                     /locus_tag="VFJ72_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479384.1"
                     /translation="MSEATKQQAPVISGHQVIAAGLASTTAAIITSRFGVAGTLLGAA
                     VTSMIITGGSAILRSYLESVTGSVRKMPYKMKAKANRAQAGRADAPDTIPGRPDLREN
                     FMGRMRAAMGWFSHLPTLRRRSIMLKGFVAAAVAFVIGMSAVYAVESGIGNSLSCGFW
                     SQCPEGAAPGLHVISGRGTGTSSSISFARPQTETAPQNETVNPSSQDASQQQDSSQQD
                     SGTPVVQDPAAGDPSATPETPTEDPAAQPETPATPSETPTATPQAPTPSSGTSESSP"
     gene            3894..4772
                     /locus_tag="VFJ72_07720"
     CDS             3894..4772
                     /locus_tag="VFJ72_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7479385.1"
                     /translation="MSVLPKKILLATDGSEDATLAARAAVEVSNGAGAELYLVHAWHA
                     VPSTRFERFIRTEMEREARHLLREQVELAETLGGTVAGAYLREGSLVEEVLAACSAVG
                     AGLIVMGTRGRGPVARLLLGSVAEGVVYRAKTPVLVVRGGDAAWPPRRVISGDDSSES
                     ALRAAELAVSIGGLFGTHNLLMRVYPRLPSIDEEGRKLDARRVDDELRREERTLEERA
                     DQIADADAARPKVCIAVGDPAKVILDEAGPEPERCLISVGKRGLGAGRMRLGSVSTKV
                     LRAARGPVLVYPQREA"
     gene            4805..5548
                     /locus_tag="VFJ72_07725"
     CDS             4805..5548
                     /locus_tag="VFJ72_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET7479386.1"
                     /translation="MDLTGVIQVFLFALLTAAATGLGAVPFIFVENMTRRWLGASNAV
                     AAGLMLAASFGLIYEGVNYSLGGVLAGAMIGLIAIVIARRVISDHDHASSFAQMSAFD
                     ARKALIIVGIMTVHSFTEGVGVGVSFGGGQALGLFITLAIAVHNIPEGLAISLVLVPR
                     GVRWDRAALWSVFSSLPQPLMAVPAFVFVEAFRPLLPYGLGFAAGAMIWMVFSELMPD
                     ALQEASANLVAVVITISIVVMIAFQVLIR"
     gene            5589..7100
                     /locus_tag="VFJ72_07730"
     CDS             5589..7100
                     /locus_tag="VFJ72_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7479387.1"
                     /translation="MNEPHDSMNPKPVLLLVLDGIRPDVLSAAIREGDAPNLGALAER
                     GEAVWDAVSVFPSITPAATAAIATGEAPARSGIVGHAWYDEDERRVIVYGAMTETVMA
                     TGPIKVFHNNVWRMNRDDLHAATLFEDLHDRGFDGACVNFPVRRGPHTHKIRMKTVEG
                     YRRGGRYLGASVKGPKEYYMGDLFYSRETGFSGRKASGGILQSVGINDEYAAQIGAML
                     IKEKAAPFNLVYFFKGDSIAHHHGLEAQRQWVTKADGYVARIFRAGGGVDRVLEDYVV
                     LALSDHGHAPLLPKRRFVDLRKIPGQNVSSGVKARFGNGTSIVLVPNGRSALLYLRDD
                     VDLRPVVEGLVGRRGVDLAVWQEGDWGVVRRLGRELRFRPGTQVTDTYGDSWDLDGDP
                     RALDLELSGDALRYGEYPDALERLWGCLHSPRCGDVVLSATPGYTFGEVSGGYHTASD
                     HGSLHASDSNVFMLASGVPAPRRITDVAPTLLRHFGTKASAQPVGDPVGDRGL"
     gene            7090..7767
                     /locus_tag="VFJ72_07735"
     CDS             7090..7767
                     /locus_tag="VFJ72_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479388.1"
                     /translation="MASSGSGRNGSSGEERQGIQPRYRRAFGCVTWINMAMLVFLAAA
                     FLLPLPFRYVGDGTIFREIPERLTFVGLILILPGMAIGAALGARTYRVERRLGIRVGA
                     GIGAIIGWTSFFVPAWLDTLPLLLLPLAALAAGLVLYALFATDKSFELRRRLVLIAAG
                     LVAVAGAFELIVDFDVLGLTGAFFSTAAAAVGGWIAGSGYARAGGDAMIPPGATIKPR
                     EPRRKPR"
     gene            7807..9636
                     /gene="uvrC"
                     /locus_tag="VFJ72_07740"
     CDS             7807..9636
                     /gene="uvrC"
                     /locus_tag="VFJ72_07740"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET7479389.1"
                     /translation="MTENGYPDRSHLPTSPGVYIFKDADDKVIYVGKAKNIRSRVANY
                     FTRSGDGRPKITELRERVSQIDFIATRTETEALVLEANLIKRHRPRFNASLKDDKSYP
                     YIVVTTGDEYPRVFATRSAHDPRHRYFGPFPSAGSVHTTLEVLNKTFPFRKCRGPEPG
                     RRSGVPCLNYHIGRSAAPCVGAVTKEGYDEIIADVIAFLEGRVDHLIRDRQQAMQEAA
                     RQMDFERAAKLRDELSALSHVRDRQQATIASEDSFDAVGAFVEGESACVQVFAVRSGQ
                     IVGRDSFLLDNYGETSAETVALQFVPQYYSTAAVPREVLVQSDEPQDILEQHLSEVRG
                     TRVEVRRPRRGDKRRILETAVRNAELGLEHEKALEEAKRNRVASTLDTLREELALPTL
                     PVRIECYDISNTMGTNSVASMVVFQGGRPAKGEYRRFKIRTVEGADDFASMAEVIRRR
                     LERLKASDEKFMPAPDLILLDGGKGQLSAVVTVLEEMKVGDDLPDIPLRSLAKRDEEV
                     YEPGRPEPVVLVRDSPELHLLQRVRDEAHRFANTYHRKLRGRAMTASVLDELPGVGPT
                     RKKRILEHFGTPEAFLNASLEELEAVPGLPGRVAREVHARVHR"
     gene            9755..>10630
                     /gene="rapZ"
                     /locus_tag="VFJ72_07745"
     CDS             9755..>10630
                     /gene="rapZ"
                     /locus_tag="VFJ72_07745"
                     /inference="COORDINATES: protein motif:HMM:NF003828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET7479390.1"
                     /translation="MNDKTPQAENPRTGARRRIVVITGLSGAGKSNALRAFEDAGYYC
                     IDNLPPAMIPAVMETAGGPEDVVVVMDIRGRRYFGEELERSLESVNSGNGWEHRVLFI
                     ESDDLSLVSRYKESRRPHPAARNGDVLEAIRSERRDLANLREKADLVVDTSGYSALDV
                     RLRFKKLAESFSSRLTISLISFGFKHGAPLDVDMLLDVRFLPNPHYDPVLRPLTGLDG
                     PVRDAVLESEDCADFLAKTSDLLSFLVPRYSAEGKTYFTLGVGCTGGRHRSVAIVEEL
                     HRRLAPLIQDTDVDFF"
BASE COUNT         1848 a         3392 c         3606 g         1784 t
ORIGIN      
        1 acagactcca tcgagaccgc gctgaagctg gccgaggggc tcgtacagat cgagaccgtc
       61 gagacggacg gcccagggga gtcgatgctc ttctcggaga gcttcacttg cactaactgc
      121 ggcgcctcga tagcggagat acagccgcgc acgttctcgt tcaatagccc gcacggagcg
      181 tgcgaccgct gcgacgggct ggggagccgg ctagagatag acccggcgct cgtcgtcccg
      241 aacgaggacc tcagcgtcaa cgacggggcg gtagttccgt gggccaactc gcagaccgag
      301 taccacggga gcgtgctcgg cgcgctttcg gagaagtacg ggatagacct cgatgcgccg
      361 tggcgggatc tgccggagga gcagaagaat ctcatcctgt tcggcactaa cggagatcgc
      421 atctacgtct cctaccgcaa ccgctacaac cggcggcgcc agtacatgac tcagttcgac
      481 ggcgtggtgg gaaatctcga gaagcgctac tccgagactg attcagagta ccggcgggag
      541 aagatcgagg agttcatgag ccacgttccc tgcccgaagt gcaagggcgc gaggctccgt
      601 cccgaagctc tggccgtcac ggtgggggag aagaatatcg cggagttcac cgcgctctcc
      661 gtcacggacg ctcaagaatt cttcgggggg gtggagttca cggccaggga gtggttgatc
      721 ggggagcggg ttgtcaagga gatccgggag cggctcggtt ttctggtgga cgtgggcctc
      781 ggatacctca ccctcgggcg ttcggcgggc accctctccg gtggcgaggc gcagcgcatc
      841 cggctcgcct cgcaggtcgg gagtgggctg gtgggcgtcc tgtacgtgtt ggacgagccg
      901 tccatcgggc tgcaccagcg ggacaaccgg cggctgttga cgacgcttga gaagctcagg
      961 gacctcggga acacgctcat cgtcgtcgag catgacgagg agaccatccg ggcggcggac
     1021 tacgtgattg acgtggggcc gggcgcggga gtccacggcg gggagatcat cgccgccggc
     1081 agcgtcgagg atatcgaggc ggaggagcgt tccgtcacgg gcgacttcct ggccggccgg
     1141 cgcaggatag agcctccgga ggagcggcgt cagccgacgg gcagcatcgg tctgatgggc
     1201 gcgatggagc acaacctcaa ggacgtggac gtggagtttc cgctggggat ctttacggcc
     1261 atcacgggtg tgtcagggtc ggggaagagc acgctggtca acgagatcct gtacaaggcc
     1321 ctcgccaacg ccgtgagccg cggcaagcac cgtcccggcc gtcacgcagg cctgcggggc
     1381 gtcgaggatg tagacaaggt catagatata gaccagagtc cgataggacg cacgccgcgc
     1441 tccaacccgg cgacctacac gaaggtcttc gaccacataa ggcagctctt cgccgcgacg
     1501 ccggaggcga agatccgagg ctacaagccg ggccggttct cgttcaacgt caagggcggt
     1561 cggtgcgagg cgtgccgggg ggacggtcag atccggatag agatgcactt cctcccggac
     1621 gtctacgtcc cttgcgaggt ctgcaagggc cgccgctaca acaccgagac cctgcgcgcc
     1681 acctacaagg gcaagtcaat ctccgacgtc ctcgacatga ccatcgagga ggcgtgcgag
     1741 ttcttcgctc ccgtccccgc gatagcgcgc cgcatggaga ctctgcggga cgtggggctc
     1801 ggctacgtcc ggctcggcca gccggccact actctgtcgg gcggcgaggc gcagagggtc
     1861 aaactggcga gcgagctcgg gaagcgggct accggcaaga cggtctacat cctggacgag
     1921 ccgacgacgg gtctgcactt cgcggacgtg gagaagctgc tgcacatcct gcaccgcctc
     1981 gtggacgccg gtaacacggt catcgtcatc gagcacaacc tggacgtcat acgctcggcc
     2041 gaccacgtca tagacctcgg ccccgaaggt ggagacggcg gcggagagat cgtggcggtc
     2101 ggcaccccgg aggacgtggc ccgtgtcgaa gagtcgcaca cgggccgctt cctgcgcggc
     2161 ctcatgccgg aggtcatggc ggcgagctag gatctacggg ccgttcgtgc cctcgaccag
     2221 ctcctccaag accgaccccg cgacgatgtt ctccaggccg cggatgacga cgctggcgtc
     2281 ctcgacgtag tcggtgtagg aaccgtcggc gcgggaccgg cggaataagc cctccagtat
     2341 ccgtccaaga cggttgattc cccactccat gtactcttcg ctgtacagag cgacgcgcac
     2401 gacggcgtcc acgctcaggg ggtcgaggtt gccgcgctcc agggtggtga agacgtagtc
     2461 gtactgctcc aggatgccgt ccacgacgtg acgggcgtcc ggcatgtctt cgctctcgaa
     2521 catgaagacg taagtgccgg cgatgatctg gagcgccttg tcctgatggt cgtactccct
     2581 gcgagagtag tcctggatca ccgcgaatag ctcagagaac ttgtcctggt cctgactttc
     2641 cacgttgtct cctccgcctg atatttgact ggatggagtc taacgcccgg aagaacccgg
     2701 cgcgactacg cgcgacagta tgagcgctca caaaaacctt cgccgaatcg ggtttttgaa
     2761 ctttaaaccg gatgagttgg ctgatagggt atcgggcatg agcgaagcaa ctaaacaaca
     2821 ggctccggtg atcagcggtc accaggtaat agcggctggc ctcgcctcga cgacggcggc
     2881 aatcatcacg tcccggttcg gggtagcggg gacgctactc ggcgcggcgg taacttcaat
     2941 gataataacg ggcggatcgg cgatcctcag gtcttacctg gagagcgtga ctggctccgt
     3001 gaggaagatg ccctacaaga tgaaggcgaa ggcgaaccgg gcgcaggccg ggcgcgccga
     3061 cgccccggac acgatacccg gccgccccga cctgcgggag aatttcatgg gccggatgcg
     3121 ggccgcgatg gggtggttct cgcacctgcc gaccctgcgc cggcgttcca tcatgctcaa
     3181 gggtttcgtg gccgccgccg tcgctttcgt tataggcatg agcgccgtgt acgccgtcga
     3241 gtcggggata gggaacagcc tctcctgcgg cttctggtcc cagtgtccgg agggagccgc
     3301 gcccggtcta cacgtgataa gcggcagggg cacgggcacg agcagttcca tctcctttgc
     3361 ccgccctcag acggagaccg cgccgcagaa tgagaccgtg aacccctctt cgcaagacgc
     3421 ttcgcagcaa caggactctt cgcagcagga ctccggcacg ccggtcgtgc aggatcccgc
     3481 cgccggagac ccgtcggcta ctcccgagac gcctaccgag gaccccgcgg cccagcccga
     3541 gactccggcc acaccgtccg agacgcccac cgcgacgcct caggcgccaa cgccctcttc
     3601 ggggacgagc gagtcctctc cgtagcgtag accgctgcca gcctccggct ccggcaaact
     3661 ctgccgcggc cggaggcttt cgtcttggcg gtccacccga ccccggactt atagattcac
     3721 gggtacgtgg cacttcgtgg atggctgcgg acgccagcgg gatgccgctg ccggaagttg
     3781 ctccaggatg acgctctctc tgacacagat tgatcaatgc cgggcacacc gcaggctgta
     3841 agatgtctgt aagaccagcg tcctggcgta tctgggccgg ataggaggag tccatgagcg
     3901 tgcttccgaa gaagatccta ctggctaccg atggctcgga ggatgcgacg ctcgccgcga
     3961 gggccgccgt ggaggtctcg aacggcgccg gagcggagct ttatctggtc catgcctggc
     4021 acgctgtccc gtccacccgg ttcgagcggt ttataaggac cgagatggaa cgcgaggcgc
     4081 ggcatctctt gcgggagcag gtcgagctgg cggagacgct tgggggaaca gttgctggcg
     4141 catacctgag ggaaggctcg ctcgtcgaag aagtgctggc ggcctgctct gcggtgggtg
     4201 ccggactgat cgtaatggga accagaggcc gcggcccggt cgcgcggttg ctgctcggaa
     4261 gcgtggcgga gggtgtcgtg tacagggcga agaccccggt ccttgtagtt cgcggaggcg
     4321 acgctgcgtg gcccccgagg cgcgtaatct ccggagacga ttcctccgag agcgccctcc
     4381 gggcggcgga gctggcggtt agtatcgggg gtctgttcgg cacccataat ctcctcatgc
     4441 gcgtgtatcc cagactgccc tcgatagacg aggagggcag gaagctggac gcgcggcgag
     4501 tagacgacga gttgcgccgg gaggagcgga ccctcgaaga gcgggcggac cagatcgcgg
     4561 acgcggacgc cgctcgaccg aaggtctgca tcgccgtcgg agacccggcc aaggtcattc
     4621 tggatgaagc cggtcccgaa cccgagcgtt gtctcatctc cgttggaaag cgaggactcg
     4681 gagccggacg catgcggctc ggcagcgtct cgacaaaggt ccttcgcgcg gcgcgaggcc
     4741 cggtgctcgt ctatccgcag cgggaggcgt gatctcgcgc cgggtaatca tatagggtga
     4801 taacgtggac ctgacaggcg tgatccaggt cttcctcttc gcgctcctga cggccgccgc
     4861 aacaggactc ggggcggtgc cgtttatctt cgtcgagaat atgacccggc gctggctcgg
     4921 ggcctcgaac gcggtcgccg ccgggctgat gctcgcggcg agcttcgggc tcatctacga
     4981 gggcgtgaac tacagtctcg ggggcgtcct ggccggggcc atgatcggct tgatcgccat
     5041 agtcatcgcc cggcgtgtga tctccgacca cgaccacgcc tccagcttcg cgcagatgag
     5101 cgcgttcgac gcccggaagg cgctgatcat cgtcggcatc atgacggtcc actcttttac
     5161 cgagggggtc ggggtcggcg tctctttcgg cggcgggcag gcgctggggc tgttcattac
     5221 gctcgccatc gccgtccaca acatcccgga aggtttggcg attagcctcg tgctggtgcc
     5281 gcgcggggtg cgctgggacc gggccgccct ctggagcgtc ttctccagcc tgccgcaacc
     5341 tctgatggcc gtccccgcct tcgtcttcgt cgaagctttc cggccgctac tgccctacgg
     5401 gctgggtttc gcggcgggag ccatgatctg gatggtcttc tccgagctga tgccggacgc
     5461 gctgcaggag gcatccgcga acctcgtcgc cgtcgtcatc acgatctcga tagtggtgat
     5521 gatcgcgttt caggtcctga tccgttagcc gcacatccgc gccgaagagc ggttaaaata
     5581 tgcgggtctt gaacgaaccc cacgatagta tgaacccgaa acctgttctg ctcctcgtcc
     5641 tcgacggcat ccgtccggac gtgctgagcg cggccatccg ggagggcgac gccccgaacc
     5701 tgggcgcgct ggccgagagg ggcgaagcag tctgggatgc ggtatcggtg ttcccgagca
     5761 taaccccggc ggcgacggcc gccatcgcca cgggcgaggc tccggcgcgc agtggcatcg
     5821 tcggacacgc ctggtacgac gaggacgagc ggcgcgtgat cgtctacggc gcgatgactg
     5881 agacagtaat ggctaccggc ccgataaagg tcttccacaa caacgtctgg cgcatgaacc
     5941 gggacgacct gcatgcagcg accctcttcg aggaccttca cgaccgcggc ttcgacggcg
     6001 cgtgcgtcaa cttcccggtg cgccgcggtc cgcacacgca caagatccgg atgaagaccg
     6061 tcgagggata ccgcaggggc gggcgctacc tcggagcctc cgtgaaaggc ccgaaggaat
     6121 actacatggg cgacctgttc tacagccgcg agacgggctt cagcgggcgc aaggccagcg
     6181 gcggcatcct ccagtccgtc ggcatcaacg acgagtacgc cgcccagatc ggggctatgc
     6241 tgataaaaga gaaggccgcg ccattcaacc tcgtctactt cttcaagggc gactccatcg
     6301 ctcaccacca cggtctcgaa gcccagcgcc agtgggtaac gaaggctgat ggttacgtcg
     6361 cccggatctt ccgggcgggc ggcggggtag accgggtgct cgaagactac gtcgtgctcg
     6421 cgctctccga ccacggacac gcgccgctcc tccccaagcg ccgcttcgtg gacctgcgga
     6481 agatcccggg ccagaacgtc tcttccggcg tgaaggcccg cttcggaaac ggcacgagca
     6541 tcgtattggt gccgaacggc cggtcggcgc ttctctacct gcgtgacgac gtggaccttc
     6601 gtccggtggt cgagggtctg gtcgggcggc gcggggtgga ccttgcagtc tggcaggagg
     6661 gcgactgggg ggttgtgcgc cggcttggcc gcgagttgcg cttccgtccc ggcacgcagg
     6721 tgacggacac ctacggcgat agctgggacc tggacggcga cccgcgcgcc ctggacctcg
     6781 aactttccgg agacgcgctg cgctacggtg agtacccgga cgcgctggag cggctgtggg
     6841 gctgcctgca ctcgccgcgc tgcggtgacg tggtgctctc ggcgacgccg ggctacacct
     6901 tcggcgaggt ctccggcgga taccacacgg cgagcgacca cggctcgctg cacgccagtg
     6961 actcgaacgt cttcatgctg gcgagcggag tccccgcgcc gcgccgcatt acggacgtgg
     7021 ctccgactct actgcgacac ttcgggacga aggcctccgc gcagccggtc ggcgaccccg
     7081 tgggggaccg tggcctctag cggctccggg cgcaacggct cctcggggga ggagcggcag
     7141 gggatacagc cgcgctaccg gcgggccttc gggtgtgtta cctggatcaa catggcgatg
     7201 ctcgtcttcc tcgcggcggc cttcctgctg ccgctgccct tccgctatgt cggcgacggg
     7261 accatcttcc gtgagatccc ggagcgtctc acgttcgtcg ggctcatcct gatactcccc
     7321 ggcatggcta tcggggcggc tctcggcgcg cgcacctacc gggtcgagcg caggctcggc
     7381 atccgcgtgg gggccggtat cggggcgatc atcgggtgga cgagcttctt cgtcccggcc
     7441 tggctggata cgctgccgct gctgcttttg cctctcgcgg ccctcgcggc gggtctcgtg
     7501 ctgtacgcgc tcttcgcgac ggacaagagc ttcgaacttc ggcggcgtct agtcctgata
     7561 gcggcgggcc tcgtggccgt cgcgggagcg ttcgaactga tcgtggactt cgacgtgctc
     7621 ggcctcacgg gagcattctt ctccacggcg gcggcggctg tcgggggttg gatcgcgggc
     7681 tccgggtacg cccgcgcggg cggcgacgcc atgatcccgc ccggcgccac catcaagccc
     7741 cgcgagccgc gccggaagcc ccgctagagg cgggagaaac atcctggcgc ggtataatct
     7801 cccccgatga cggagaacgg ctacccggac cgctcgcacc tcccgacctc gcccggcgtc
     7861 tacatattca aggacgctga cgataaggtc atctatgtcg gcaaggcgaa gaatatccgc
     7921 agccgggtgg cgaactactt cacccgctcc ggcgatggcc gcccgaagat aacggagctt
     7981 cgcgagcggg tctcgcagat agacttcata gcgacacgga cggagaccga ggcgctggtg
     8041 ttggaggcga acctgatcaa acgccacagg ccgcgcttta acgcctccct gaaggacgac
     8101 aagagctacc cgtacatagt cgtcaccacc ggcgacgagt acccgcgcgt cttcgccacg
     8161 cgcagcgcgc acgacccgcg ccaccgctac ttcgggccat tcccgagcgc gggctcggta
     8221 cacacgacgc tggaagtgct gaacaagact ttcccattcc gcaagtgccg ggggccggag
     8281 ccggggcgca ggagcggcgt cccgtgcctg aactaccaca tcgggcgttc ggccgcgccg
     8341 tgcgtcgggg cggttacgaa ggaggggtac gatgagatca tcgcggacgt catcgccttc
     8401 ctcgaaggtc gagttgacca cctgatccgg gaccgccagc aggcgatgca ggaggccgcc
     8461 cgccagatgg acttcgagag ggccgcgaag ctgcgcgatg agctatccgc tctctcgcac
     8521 gtccgggacc ggcagcaggc gaccatagcc tctgaggact cgttcgatgc ggttggagca
     8581 ttcgtggagg gcgaatcggc gtgcgtgcag gtcttcgcgg tgcggagcgg ccagatcgtg
     8641 ggccgcgact ccttcttgct ggacaactac ggcgagacaa gcgcggagac cgtagccctg
     8701 cagttcgtgc cgcagtacta ctcgaccgcc gccgtcccgc gcgaggtgct ggtccagtcc
     8761 gacgagccgc aagacatatt agagcagcat ctctccgagg tgcgcgggac gcgggtcgag
     8821 gtgcggcgtc cgaggcgagg agacaagcgc cgcatcctgg agaccgccgt gcgcaatgcg
     8881 gagctcggcc tggagcacga gaaggcgctg gaagaggcga aacgaaaccg ggtcgcctcc
     8941 acgctcgaca cgctgcgcga ggagcttgcg ctgccgacgc tgccggtcag gatcgagtgc
     9001 tatgacatct ccaacacgat ggggacgaac tccgtcgcct cgatggtcgt cttccagggc
     9061 ggtcgtccgg ccaagggcga gtaccggcgg ttcaagataa ggaccgtcga gggggcggac
     9121 gacttcgcga gcatggccga ggtcatccgg cgccgcctcg aacgcctcaa ggcgagcgac
     9181 gagaagttca tgcccgcgcc ggacctgatc cttctggacg gcggcaaggg ccagctctcc
     9241 gccgtcgtca cggtcctgga ggagatgaag gtcggtgatg atctgccgga catcccgctg
     9301 cgttccctcg ccaaacggga cgaggaggtc tacgagccgg gccgcccgga gccggtcgtg
     9361 ctggtgcgcg actcccccga gctccacctg ctgcagcgtg tgcgcgatga ggcacaccgt
     9421 ttcgccaaca cctaccaccg aaagctccgg ggccgggcga tgaccgcctc tgtcctggac
     9481 gagctgcccg gcgtcggtcc gacccggaag aagagaatac tggagcattt cggcaccccc
     9541 gaggcgttcc tgaacgcctc cctggaggag ctggaagccg tgccgggcct gccgggcagg
     9601 gtcgcccgcg aggtccacgc cagagtgcac agatagcatg aagccgttaa attcccttta
     9661 aatatacggg aatccgttgc aagtatggaa tcgtggtgct actctcgaac cgcaagatga
     9721 tctttctacc tcaggagaga ggggagaccc cggcgtgaat gataagacgc cgcaggcgga
     9781 gaaccctcga accggcgcgc ggcgccgcat cgtcgtcatc accgggctct ccggcgctgg
     9841 taagtctaac gccctccggg ccttcgagga cgcgggatat tactgcatag ataacctgcc
     9901 gcccgccatg atcccggccg ttatggagac cgccgggggg ccggaggacg tggtggtcgt
     9961 gatggacatc cggggacgcc ggtacttcgg cgaagaactc gaacggagcc tggagtcggt
    10021 taactcgggg aacggatggg agcatcgcgt cctgttcata gagtccgacg acctctcgct
    10081 cgttagccgc tacaaggaga gccgccgccc ccacccggcg gcgcgcaacg gcgacgtgct
    10141 ggaggctata cgctccgagc gccgggacct ggcgaacctc cgcgagaagg cggacctggt
    10201 cgtggacacc tcgggatatt ccgctctcga cgtgaggttg aggttcaaga agctggccga
    10261 atccttttcg agccgcttga cgatcagcct catctccttc ggcttcaagc acggcgcgcc
    10321 gctcgacgtg gacatgctcc tggacgtgcg cttcctaccg aacccgcact acgatcccgt
    10381 ccttcggccc ctcaccggcc tcgacggccc ggtccgcgac gcggtactcg aatctgagga
    10441 ttgcgccgat ttcctggcga agacaagcga cctgctctcg tttctcgtcc cgcgttactc
    10501 tgcggagggc aagacgtact tcacgctagg ggtgggatgt accggcggcc ggcaccgctc
    10561 cgtcgccatc gtggaggaac tgcaccggcg cctggctccc ttgatccagg atacagacgt
    10621 tgatttcttt
//
LOCUS       DASSMB010000127        18807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1166940, whole genome shotgun sequence.
ACCESSION   DASSMB010000127 DASSMB010000000
VERSION     DASSMB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 18807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18807
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166940"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3055
                     /locus_tag="VFJ72_07750"
     CDS             <1..3055
                     /locus_tag="VFJ72_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET7479391.1"
                     /translation="EIQTPVFLSAEVEIPPDWEGEPVEVELWLGGEGFVRLSNGVTGG
                     LNPFHRAFPVAERARVGEKVRIEAEVVSKGLWGSQVAEPRIERAALVVPETSVRSLLR
                     DLAVVAAACEELDGHDAVPHLLDAVDAAFAALAPHWPSESGAAVARYLRGYEYPAGDG
                     LSSLPSELASQIAGGPRGMRAMWAVPQPGRPLEPLPDTARRALREARKLLAGRLEEIK
                     REYPPVGRLALTGHAHIDLAWLWPLAETRRKARRTFSSVLSLMDRYEDFTFNQSSAQL
                     YAWIEEDDPDLFERIRARVAKGRWETVGGMWCEPDCQIPSGESFVRQLLHGQRFFEEK
                     FGRRSTVAWLPDAFGFSPGIPQLLRGAGITGFFTAKLKWSATNRFPLDLFHWEGLDGS
                     RVTAHFTRNPGMDYNGDIAPFDLLGVWRNFKGKRRHPESLFPFGWGDGGGGPSEEMLE
                     SRARLGDFPALPRLRMGRVDEFFESLPDEDLPKWVGELYLELHRGTLTTQGLVKKLNR
                     EAEHRLLEAEAFATIAALHGAGYPSEEFDRLWKILLLNQFHDILPGTSIAEVYREAHG
                     QLREVVGGAEKLRNGSLRAVACGSGDGGVTLVANAGLAPRPLSALLPEGVSAVAGRDG
                     EPLPVQRTAEGLLVHAPDVRVPGLGGITLRRVEGAGAEVRSPVLVEDSGDGVILENEL
                     LRVEIHTDGTLHRVYDCEMDREVLDGRGNQLQAYVDRPANWDAWDVDEGYELEGEEIP
                     AAERIEVVEEGPLRAAVRVVRRWRSSRITQTYRLHTGSRRLDIVTEIEWHEREILLRA
                     LFPLNIRSATATCETMYGAQDRPTHANTSWDAARFEVSAHRFADLSEPGYGVALLNDG
                     KYGHSARDNVLGLSLLRSPMYPDPFADEGEHRFTYSLFPHPGDWTEAGVVEEAFALNS
                     PLVAVLGSETFAGSGFVSVEGLKLALGSLKWAEDGRGGILRLYEPHGARGAALLRFAH
                     QIRSIERVNLLEEPEGDAPAIRDGVVRMEVRPFEVITLRLRLRAG"
     gene            3086..3961
                     /locus_tag="VFJ72_07755"
     CDS             3086..3961
                     /locus_tag="VFJ72_07755"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7479392.1"
                     /translation="MEHRRLGRLGRENSVLIFGGAALSEVSEDAADRAISAALDAGVT
                     HFDTAADYGDSELHYGRWMPEIRDRIFLSTKTGLREKDAARRQIEASLERLRVDGVDL
                     LQLHAVGDLEDLDRATGAGGSLEAAIEAKEEGLVGAIGITGHGHGAPATHLEALRRYP
                     FETVLTPWNYILSTDESYRTDFEALVEEVERQDAGLMIIKTISRRNWPEGDPTGDQKY
                     ATWYEPFDQQQYVDAAVSFVLSYEEVAGLAMAGDVSLAPMMLAAERRRMSREEAEEIL
                     SRDRDYSSPFISMPF"
     gene            4007..4966
                     /locus_tag="VFJ72_07760"
     CDS             4007..4966
                     /locus_tag="VFJ72_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7479393.1"
                     /translation="MKTRKFGKTDMDITPVGFGSWAIGGSGWAAAWGPQDDEEAVGAI
                     QRAVELGINWVDTAAVYGLGHSEELVARALKNIPEADRPNVFTKCSLVWDDDANISNV
                     LKKDSVKRECEDSLRRLQTDVIDLYQIHWPNPDEDIEEGWQAMAELKEEGKVRHIGVS
                     NFDVGQMKRANEIAPVETLQPPYSMLKREVEAEILPYCEENDIGVIVYSPMRSGLLTG
                     KMTPERIQNLPSDDWRRNADDFQEPRLSKNMAVVELLKEIGARHDKSPAEVAIAWTLR
                     HPAVTAAIVGGRRPDQVDGTIGAADFRLSEDEVGEIEAALPKV"
     gene            complement(5313..6845)
                     /locus_tag="VFJ72_07765"
     CDS             complement(5313..6845)
                     /locus_tag="VFJ72_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479394.1"
                     /translation="MRLTVVSILIGFSSYAQVLINKRKRAEAKVQRLNSVLENRVTER
                     TAQLTTTVEELKTNQRMLQESEERFRSIFEQAAVGIAQVSLDGRLLRVNGKLCQITGY
                     TREEICELPFQDIICPNDVDADFEDLRRMLSSEIETYKMERRYSRADGSPIWINLTVS
                     LGSASVEESEYCIVVVEDITRRKQAENELTRLASFPRLTPIPIIEAIPDEDLNYVNPA
                     AADLFPDLNWLRLKHPMLAGLDSVAAELRSDEKESCVREVKVNGDCYHQLIARVPGSE
                     NLRIYGIDITERKRSESAMREIRESERRRLARDLHDEALQDLIYALHEVEIMQITPDY
                     GRRNPALEGIFSALQRSVDGMRGAIHDLRAGDVQNQPFPLLLESLIEQRRRMTPNCEI
                     ELIVQDDFPSVNLGAPGMDLLRIFQEALANVRRHAQARHARVTLQLDGDELQAEIADD
                     GIGFEPGATYSTGVTGMRERATALNGELEIQSDPGKGTRLRFQTALSGLLEYHEDPKT
                     YR"
     gene            7494..8867
                     /locus_tag="VFJ72_07770"
     CDS             7494..8867
                     /locus_tag="VFJ72_07770"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET7479395.1"
                     /translation="MDFPEGFLWGTATAAYQVEGAVNEDGRGPSIWDTFSHTPGKVYR
                     GDTGDIACDQYHRLEQDLDLMAELGINAYRFSVAWPRIQPEGGGAVNQKGLDYYRRLV
                     DGLRQRGIQPMLTLYHWDLPQALEDRGGWTSRETSDRFAEYAGVVYEALGDTVPYWIT
                     LNEPWVAAWLGHGYGVHAPGHADTAMALAATHHLLLGHGLALQAMRSAAIPGDENQFG
                     ITLVLQPARPSRDREADADAVRRVDGHANRLYLDPLFHGEYPDDLLSHYRERGVELPS
                     VQDGDMDVISGDLDFLGVNYYFRHAVRDAPDEEPKAVPFSELNARVVIPHDAEKTAMG
                     WPVEPEGLTQVLVRIKEEYADLPIYITENGRAVHDYVDPEGGVDDEERISYLDAHFRA
                     AHEAIGRGVDLKGYMVWSFLDNFEWAEGYSKRFGIVYVDYGTQRRVPKASARWYSDVI
                     RRNGLED"
     gene            8880..9065
                     /locus_tag="VFJ72_07775"
     CDS             8880..9065
                     /locus_tag="VFJ72_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479396.1"
                     /translation="MNYRELDGTGMRVSEISAGVWTFGSGFSLADLGGGTRRGLAGMM
                     KRLPAVFRREAVEGSAA"
     gene            9062..10036
                     /gene="galE"
                     /locus_tag="VFJ72_07780"
     CDS             9062..10036
                     /gene="galE"
                     /locus_tag="VFJ72_07780"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7479397.1"
                     /translation="MKLLVTGGAGFIGSVVASQLVAAGHETVILDNLSKGRRSAAPEG
                     ARLIEADLLNAERTREVLGEGFDGVLHFAALSVVGESVEEPERYYRNNVCGTLNLLEA
                     MVAAGVSRLVFSSTAAVYGEPDEVPIPETAPAVPTNPYGASKLAVDRLIGAVAEARGI
                     CATSLRYFNVAGASGNSGEDHGPETHLIPLVLKSAAGKRDSVKIFGTDYPTRDGTAIR
                     DYIHVEDLGRAHLLALEDARAGEHRVYNLGNGKGFSVREVIDVARRVTGRPIEAVEAP
                     RRAGDPPELVASSEKIRAELGWKPEKPSLEAMISDAWTWMQEHTGGYG"
     gene            10336..10911
                     /locus_tag="VFJ72_07785"
     CDS             10336..10911
                     /locus_tag="VFJ72_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7479398.1"
                     /translation="MANELQGKRVAILLAPVGTEQVEFVQPKQAVEDAGGEVDVVGIE
                     TGEVQTMNSDVNPGDTFAVEKTFSEVSAEDYDGLIVPGGTVGADNLRGSEEAVGFVRG
                     FFEQAKPVGAICHGPWTLVEADVVKGRTLTSYPTLKTDIKNAGGNWVDEEVVVDQGLV
                     TSRNPDDLPAFCAQIVEEIAEGEHAEQKRSA"
     gene            11163..11774
                     /locus_tag="VFJ72_07790"
     CDS             11163..11774
                     /locus_tag="VFJ72_07790"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7479399.1"
                     /translation="MAQRIDGSIEIEAPVAKVYDYWKTLENLPKFMSNIEEVRPTGPD
                     TTHWIVRGPLGYKAEFDAKTTQNEPNQAIAWNTENGDVETSGQVRFQELTPNRTRVEV
                     QMNYWNPPGGKVGEAASRVTSGPKTILVQDLQNFKDIMEGTATPEEVQQRPSAADVHS
                     GAITFLTSGTGLLVVGGSLVLWLLLRRRGGSSSRKKSRIIFEF"
     gene            complement(11891..12214)
                     /locus_tag="VFJ72_07795"
     CDS             complement(11891..12214)
                     /locus_tag="VFJ72_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET7479400.1"
                     /translation="MTGLADRTCVPCRGGVPPLGGEVLNALASQVPEWEVVAEHHLRR
                     EFRFTNFREALDFVNRVGELSEEQGHHPDISFGWGRAELIVFTHAIDGLTESDFILAA
                     KVDRL"
     gene            complement(12226..12771)
                     /locus_tag="VFJ72_07800"
     CDS             complement(12226..12771)
                     /locus_tag="VFJ72_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7479401.1"
                     /translation="MPHPWHDVPIGEEAPREFNALIEIPKGSKVKYELDKETGLLLVD
                     RVLYASIHYPANYGFIPRTLGDDDDPLDVLVLMQETVFPLSLLRAKPIGMMHMVDEGQ
                     DDEKIICVHLDDPEFRSYEHYKELPEHRLDELRSFFQDYKKLEDKEVNVEDFADPEEA
                     IEAVQHAMDLYDEKFPQNGKS"
     gene            complement(12894..12976)
                     /locus_tag="VFJ72_07805"
     tRNA            complement(12894..12976)
                     /locus_tag="VFJ72_07805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12941..12943),aa:Leu,seq:caa)
     gene            13180..14445
                     /locus_tag="VFJ72_07810"
     CDS             13180..14445
                     /locus_tag="VFJ72_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7479402.1"
                     /translation="MYRVRRVGMLAAVGVVSALLIVGCGGGSGGSQGGGGGGDSVKIV
                     SDLPLQGANRAQTTTMNNAIELALKERKGKAGDVTVELEKKDDATAQAGQWDPSKCAE
                     NAQQAAQDDSVVGWIGPFNSGCAATEIPTLNEAGLGMISPANTYIGLTKKGGEPDEPD
                     KYYPTGKRNYARVIVADDKQGAAGAQLLQQEGAKSVYILDDKQTYGKGLADQFEKAAK
                     NSGIQVLGREGIDGKASNYRSLMSKISQSNPDAIYFGGIIENNAAQIIKDKVGAGMSN
                     EDVLFVGPDGIFVDSLIDQAGKDAEGIYVTFGGLPESELSAKGQAFVKKYRSTYDDDI
                     QPYTAYAYEAANVMLDAVEKASKKAGGVPDREAVVKEIFATKDYNGVLGKWSFDKDGD
                     TTLTKLSIQKVENGKFKFQRTVDVADLVQ"
     gene            14524..15609
                     /locus_tag="VFJ72_07815"
     CDS             14524..15609
                     /locus_tag="VFJ72_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7479403.1"
                     /translation="MTTGEPTKSRSGVLLDSVRSSSWQSRIAVALIAALLILLLVQAA
                     QEPGEFASQLVIGITNGAIIALIALGYTLVYGIIELINFAHGDNFMLGSFVGLTVLSG
                     TFFGIGLFAPINGESSAFLKVIGIGIALVVAMAACGLINMGIERVAYKPLRNSPRLAV
                     LITAIGMSFILQNVGLVWKGPTQVPFPELISNANVFNESVVAFRWRDLFVIVVTVPLL
                     AGLSYFIRSTKQGKAMRAVAQDKEAASMMGINVNRTISITFLLGGILAGASGVMFGLF
                     NGITVFSVGFTQGLYAFTAAVLGGIGNLTGAVVGGFLIGIIGSMSDQYIGVRWTAIVI
                     FAVLILVLVFRPRGLLGDRSAVQGGGE"
     gene            15611..16777
                     /locus_tag="VFJ72_07820"
     CDS             15611..16777
                     /locus_tag="VFJ72_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479404.1"
                     /translation="MAARLKRYWLPIVLIVAALLFPFLDTALDAGLMYPVITIAIYVM
                     LALGLNIVVGFAGLLDLGFVAFYALGAYVVGWFASSQFNQVSFSFGSTAVSLSGGSPP
                     GIHLSFWILLVLAGAFTALCGVIIGFPTLRLRGDYLAIVTLGFGEIIPRFFQNGDDLG
                     GFNLTNGTIGIKAIDSPGIPFMPDSLSTLQRFSSLDLNPWYYTILILVLITIYVNVRL
                     QDSRLGRAWISVREDETAAAAMGINPVTTKLWAYALGAVFGGLAGAFYGAFTKNIFPD
                     AFQFNVSILILCMVILGGMGNIYGVILGAIALQGLNFYLLPKINDWVHAIGNLVGSPL
                     LSGADIPKYNFFLFGVILVLMMLLRPEGIIPNRQRQEELRGGDEEPAATEPARV"
     gene            16770..17597
                     /locus_tag="VFJ72_07825"
     CDS             16770..17597
                     /locus_tag="VFJ72_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479405.1"
                     /translation="MSEQNGNTTSGAPNGLAKNVSNIMEARGITKVFGGLVAVNSVDF
                     DIPERSIVSLIGPNGAGKTTFFNMVSGLYQPTSGTFSLVGRDITRLKAHQRAQLGIGR
                     TFQNVRLFGTMSAQDNVLSGMHTRLKGNILGSIFRTPATRREESEAREKAQELLRFVG
                     IQGRETFAKNLSYGDQRRLEIARALASEPKLLLLDEPTAGMNPQETAKLTDLMGRLRE
                     ERGIAVLLIEHEMRVVMSISDRVTVLDHGEKISEGAPADVRQDPKVIEAYLGTAKTG"
     gene            17599..18174
                     /locus_tag="VFJ72_07830"
     CDS             17599..18174
                     /locus_tag="VFJ72_07830"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7479406.1"
                     /translation="MGQTFDKIPDEFAGWFEQQPVFFVATAPADPETHVNVSPRGLDT
                     FRVIGQNRVAWLDLTGSGVETIAHLKVEEAGGRITLMFCAFEDSPRIVRLYGRGRVHE
                     PGDDAHGELRSHFPDLPGARAIIDVTVDRVSTSCGFGVPLMEFMGHRDELIESARRKG
                     ETMMEAYRARKNAESIDGLPGLESATLVETN"
     gene            18176..>18807
                     /locus_tag="VFJ72_07835"
     CDS             18176..>18807
                     /locus_tag="VFJ72_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479407.1"
                     /translation="MALLEVENMHSYYGNIHALRGVSLTVEEGEVVTLIGSNGAGKTT
                     TLRSIHGVLPPREGKVIFDGQEIQGVPAHDMISKGIAQSPEGRKIFPRMTVMENLEMG
                     AYHRNDRAGIKQDIDRVFELFPRLQERIKQEAGTMSGGEQQMLAIGRALMSRPKLLLL
                     DEPSMGLSPVLVERIFDIIKEINAQGTTILLVEQNANIALEIATRGYVLET"
BASE COUNT         3528 a         5717 c         6186 g         3376 t
ORIGIN      
        1 ggagatccag acgccggtat tcctgtcggc ggaggtggag atcccaccgg actgggaagg
       61 cgagccggtg gaggtcgagt tgtggctggg cggcgagggt ttcgttcggc tctcgaacgg
      121 cgtcaccgga ggtctaaacc cattccaccg cgccttcccg gtcgcggagc gggcgcgggt
      181 cggggagaag gtccgcatag aggccgaagt agtatcgaag gggttgtggg gctctcaagt
      241 cgccgagccg cgcatcgagc gggccgcgct ggtggttccc gagacgagcg tgcgatctct
      301 gctacgggac ctcgccgtgg tcgcggcagc ctgcgaggag ctggacggcc acgacgccgt
      361 accgcatctg ctcgacgctg tggacgccgc cttcgccgcg ctcgctccgc actggccgtc
      421 ggagagcggc gcggccgtgg cgcggtatct gcggggctac gagtatccgg cgggggatgg
      481 gctctccagc ctgccgtccg aacttgcctc gcagatcgcg ggcggtccgc gcgggatgcg
      541 cgccatgtgg gccgtgccgc agccgggacg acctctggaa ccgctcccgg acacggcgcg
      601 gcgggcgttg agggaagccc gaaagctcct cgcgggccgt ctggaagaga tcaagcgaga
      661 gtatccgccg gtcgggaggc tcgcgctgac cggacatgcg cacattgacc tggcctggct
      721 ctggccgctc gcggagacgc ggcgtaaggc gcggcggacc ttctccagcg tcctgagctt
      781 gatggaccgt tatgaggact tcacgttcaa ccagtcctcg gcgcaactct acgcctggat
      841 cgaggaggac gacccggatc tgttcgagag gatcagggcg cgggtggcga agggacggtg
      901 ggagacggtc ggggggatgt ggtgcgaacc ggactgccag atcccatccg gcgagtcctt
      961 cgtccggcaa ctcctgcacg gccagcggtt cttcgaagag aagttcggac gccgctcgac
     1021 cgtggcctgg ctcccggacg ccttcggttt ctcgcccggc atcccgcaac tgctgcgcgg
     1081 cgcgggaatt accggcttct tcaccgcgaa gctcaagtgg agcgcgacga accgctttcc
     1141 gctagacctc ttccactggg aggggctgga cggcagccgt gtcaccgccc acttcacccg
     1201 caaccccggc atggactaca acggcgacat agcgcccttc gaccttctcg gcgtctggcg
     1261 caacttcaaa gggaagcgtc gccatcccga gagcctgttc cccttcggct ggggcgacgg
     1321 tggcggaggt ccgagcgagg agatgctcga aagccgtgcc cgcctggggg acttccccgc
     1381 gctgccccgg ctgcgcatgg gccgcgtgga cgagttcttc gagagcctgc cggatgagga
     1441 cttgccgaag tgggtcggag agctgtacct ggagctacac cggggcacac tcaccacgca
     1501 ggggctcgtc aagaagctca accgcgaggc cgagcaccgg ctgctggagg ccgaggcgtt
     1561 cgcgaccatc gccgcgctcc acggcgccgg ctatccctcg gaagagttcg accggctctg
     1621 gaagattctg ctcttgaacc agttccacga catcctgccg ggcacgtcca tcgccgaggt
     1681 ctacagggag gcgcacgggc agctacggga ggtcgtcggc ggcgcggaga agctgcggaa
     1741 tggctcgctc cgcgccgtgg cgtgcggaag cggtgacgga ggcgtgacgc tcgtcgccaa
     1801 tgccgggctc gctccacggc cgctctcggc gctgctgccg gagggtgttt ccgcagtcgc
     1861 cggtcgagac ggagagccgc tgccggtaca acggacggcc gaagggctgc tggtacacgc
     1921 gcccgatgta cgggttccgg ggctcggagg gatcacgctc cgcagggtgg agggcgcggg
     1981 ggcggaggtc cggtcacctg tcctggtcga ggattcgggt gatggcgtga tccttgagaa
     2041 cgagcttctt cgggtcgaga tccacaccga tggaaccctg catcgcgttt atgactgcga
     2101 gatggaccgt gaggtcctgg acgggcgcgg gaaccagctc caggcttacg tagacaggcc
     2161 cgcgaactgg gacgcctggg acgtggacga gggatacgag ctagaaggag aggagatccc
     2221 ggcggccgag aggatcgagg tcgtcgaaga ggggccgttg cgcgcggcgg tgcgcgtcgt
     2281 gcgccggtgg cgaagctcgc gcatcacgca gacgtaccgg ctgcacaccg gctcccggcg
     2341 gctggacatc gtcacggaga tcgagtggca cgagcgagag attctcctgc gcgcgctctt
     2401 cccgctgaac atccgctctg cgacggctac ctgcgagacg atgtacggcg ctcaagatcg
     2461 tccgacgcac gccaacacgt cctgggacgc ggcccgcttc gaggtctccg cgcaccgctt
     2521 cgccgacctc tccgagccgg gctacggcgt cgccctgctg aacgacggga agtacggcca
     2581 cagcgccagg gacaacgtgc tcggcctgag cctgctccgt agccccatgt acccggaccc
     2641 gttcgcggac gaaggggagc atcgcttcac gtacagcctc tttccgcacc ccggagactg
     2701 gacggaggct ggtgtggtgg aggaggcatt tgcgttaaac agtccgctcg tggcggtttt
     2761 aggttcggaa acgttcgccg ggtctggatt cgtgagcgtg gaggggttga agctcgcgct
     2821 ggggagtcta aagtgggcgg aggatggtcg aggtgggatc ctgcgcctct acgagcccca
     2881 cggcgcgcgc ggcgcggctt tgctgcgctt cgctcatcag atacgcagta tcgagagagt
     2941 caacctgctg gaggagccgg agggtgatgc cccggcgatc agggacggtg tggtaaggat
     3001 ggaggtgcgg ccgttcgagg tgataacgct gcgcctgcgg ttgcgcgcgg ggtaagatag
     3061 tcggcaggcg aagagaggaa cgaggatgga acatcgcagg ctcggacggc tggggcggga
     3121 gaactcggtg ctcatcttcg ggggcgcggc gctctccgag gtgagtgagg acgcggcgga
     3181 ccgcgcgatc tccgccgcgc tcgacgcggg cgttacgcac ttcgataccg ccgccgatta
     3241 cggcgactcg gagcttcact acgggcgatg gatgccggag ataagggacc ggatcttcct
     3301 ctccacgaag accggcctgc gcgagaaaga cgcggcgcgg cggcagatcg aagcgtcttt
     3361 ggagcgcctg cgggtggacg gcgtggacct cctccagctc cacgctgtcg gcgacctcga
     3421 agacctggac cgcgccaccg gcgccggcgg ctccctggaa gccgctatcg aggcgaagga
     3481 agaaggactc gtcggggcta tcggcatcac cggccacgga cacggcgctc ccgcgacgca
     3541 cctggaggcg ctgcggcgct acccgttcga gacggtgctt acgccctgga actacatcct
     3601 ctcgacggac gagagttacc ggacggactt cgaggcgctg gtcgaggagg tagagcgcca
     3661 ggacgcgggg ctcatgatca tcaagactat ctcgcgccgt aactggcccg aaggcgaccc
     3721 gaccggcgac cagaagtacg cgacatggta cgagcctttc gatcagcaac aatacgtaga
     3781 cgccgcggtc tctttcgtgt tgtcttacga agaagtcgct ggcctcgcta tggccgggga
     3841 tgtgtcgctg gcgccgatga tgcttgcggc cgagcggcgc cggatgtcac gtgaggaggc
     3901 cgaagagata ctctcacgtg accgcgatta ctcctcgccg tttatctcga tgccgttcta
     3961 gacatatata gcggcgtttg cgtaagcgta tttagcggag gccgttttga agacgcgcaa
     4021 gttcggcaag acagacatgg acatcacgcc ggtaggattc ggtagctggg ctatcggcgg
     4081 cagcggatgg gccgccgcct gggggccgca ggacgacgag gaagccgtcg gcgccataca
     4141 gcgggccgtt gaactgggta tcaactgggt ggacaccgcc gccgtatatg gtctgggtca
     4201 ttccgaggaa ctcgtcgccc gggcgctaaa gaacatcccg gaggctgacc ggccaaacgt
     4261 cttcaccaag tgctcgctgg tatgggacga cgacgccaac atctcgaacg tcctgaagaa
     4321 agattccgtc aagcgggagt gcgaggacag cctcaggcgc ttgcagacgg acgtgatcga
     4381 cctgtaccag atccactggc ccaaccccga cgaggatatc gaagagggct ggcaggcgat
     4441 ggcggagctg aaggaagagg gcaaggtccg gcacatcggc gtctccaact tcgacgtggg
     4501 gcagatgaag cgggcgaacg agatagctcc ggtcgagacg ctacagccgc catactcgat
     4561 gctcaagcgc gaggtcgagg cggagatact gccgtactgc gaggagaacg atataggggt
     4621 catcgtctac tcgccgatga ggagcggcct gctcacgggc aagatgacgc ccgagcgcat
     4681 ccagaacctc ccgtcggatg actggcgccg caacgccgac gacttccagg agccgaggct
     4741 ctccaagaac atggcggtag tcgagctgct gaaggagatc ggagctcgcc acgacaagtc
     4801 tccggcggag gtagccatag cgtggacgct gcggcatccg gcggtaacgg cggccatcgt
     4861 gggtggccgc cggccggatc aggtcgacgg caccatcggc gccgcggact tccgtctctc
     4921 ggaagatgag gtgggggaga tagaggccgc tctcccgaag gtctaaagct catataagtc
     4981 gtcacctctg atgagccact gtctatacgg tagctggaac gagcgataaa cggcgcgtcg
     5041 ctccagaaac gagaataccc ggcactctat ggatacacag gggagcgggg atcctgacgc
     5101 aggaacgcgg gcgaagggaa ggatagagta cacactcttg gaggggggta tgccgcccat
     5161 ccaacctaaa cccgacgccc gctataagta tcaattatat catgttgtcg tggggaggtg
     5221 caaaagtaac tttcgccttt ccctctactt cagagatcaa ggcgcagccg catcggcggt
     5281 tagccgcgcc ccaatcgcgc tctgaatgaa gactacctat atgttttggg atcttcgtgg
     5341 tattcgagta gaccggataa cgcggtctgg aaccgtagcc gcgttccctt gccgggatcg
     5401 ctctgaatct ccagttctcc gttgagcgcc gttgcccgct ctcgcatacc cgtgactcct
     5461 gtactgtagg tagctccggg ctcaaatcct atcccgtcgt cggcgatctc agcctgcaac
     5521 tcgtctccat cgagttgcag ggttacacga gcgtgccggg cttgagcatg tcgccggacg
     5581 ttcgccagcg cctcctggaa gatccgcaac aggtccatcc cgggtgcccc taggttcacc
     5641 gatggaaagt catcttgcac aataagctct atctcgcaat tcggggtcat ccggcgtctc
     5701 tgctctatca gcgactccag caacaggggg aacggctggt tttggacatc acccgctcgc
     5761 aggtcgtgta tcgcgcctcg cataccgtcc accgagcgct gcagagcgct gaagatcccc
     5821 tccagggcgg gatttcgtct gccgtaatca ggtgtgatct gcattatctc gacctcgtgc
     5881 aaggcataga tgaggtcctg tagagcttca tcgtgcaggt cgcgggccag tcggcggcgc
     5941 tcggactcac ggatctcgcg catagcgctc tccgaccgct tgcgctccgt gatgtcgatg
     6001 ccatagatgc ggaggttctc gctacccgga acgcgggcta tcaactggtg atagcagtcg
     6061 ccgttgactt tgacttcgcg tacgcaactc tctttttcgt cgctgcggag ctcggcggcg
     6121 accgagtcca gcccggccag catcggatgc tttagccgca gccaattcag gtctggaaag
     6181 aggtcggccg ctgccggatt gacatagttg aggtcctcat ccggtatcgc ctcgatgatc
     6241 gggatcgggg tcagcctggg gaaggaagcg agccgggtta gctcgttctc cgcttgctta
     6301 cggcgggtaa tgtcctcgac cacgacgata caatattcgg actcctccac gctcgcgctc
     6361 ccgagcgaga ctgtcaggtt gatccagatc ggagagccgt ccgctcttga gtagcgcctc
     6421 tccatcttgt aggtctctat ctcgctggac aacatccggc gtaaatcctc gaagtccgca
     6481 tccacatcgt tcggacagat tatgtcctgg aaaggcagtt cgcatatctc ctctctggtg
     6541 tagccagtga tctggcagag cttcccgtta actctcagca agcggccatc caggctaacc
     6601 tgcgcgatgc cgacggcagc ctgctcaaag atcgagcgga acctctcttc gctctcctgc
     6661 agcatccgct ggttggtctt tagctcctcg acagtggtcg tcagttgcgc ggtgcgctcg
     6721 gtcactcggt tctccagaac actgttgaga cgctgaacct tcgcctccgc ccgcttgcgc
     6781 ttgttgatga gaacctgagc ataagagctg aaaccgataa ggatgctcac gaccgtcagc
     6841 cgcatccata tctcgttcgg atcgtagaaa ggtaatatcc ttctgctgaa gctggcgtga
     6901 tggaataaag acggcagcaa ggattcaact gaggcttcgg cgatccagta gaagactgct
     6961 agcccgattc cgatgagtac gagtttgttt gtcgcgagtg aatgtttcag cttctcatac
     7021 cacatcgcgc aaccccataa cctctccgac tacgcccgcc gcaccttcgt ccaccgcgcc
     7081 tgaaatgtat cccttcgcaa cacagatcta ctcattcata actcttcggg tgcttgcatg
     7141 cgatacaacc aacccactac ccgaaatcta tagtagctag taccatagta gctagtaggt
     7201 ccaaattcag tatcctggga taccaagtgt accaagtgcg cggccgctcg aagagagcgt
     7261 tgcatgggga gaactcttga atggcgctca gcgtacgctc tgaccgtcgc attctcgacc
     7321 ggcgcgcatc tcaggcgctc tttaatgaag gagaccgaag cggttcgtgt gaccctccga
     7381 catggcgtgt gccgaagctg ctcgactgtc ctcggctgtg ctcgactggc ggggaaggtg
     7441 gcagggcgtt tagactggcg tttcgtggac tagtagggtt tggaggagac ggcatggatt
     7501 tccccgaagg cttcttgtgg ggtacggcga cggcggccta ccaggtagag ggagccgtga
     7561 acgaagacgg tcgtggtccc tccatctggg ataccttctc gcacaccccc ggcaaggtct
     7621 accgcggcga caccggagac atcgcgtgcg accagtacca tcgcctcgaa caggacctgg
     7681 acctcatggc ggagctaggg ataaatgcgt accgcttctc cgttgcctgg ccccgcatac
     7741 agcccgaggg cggtggcgcc gtcaatcaga aaggtctcga ctactatcgg cggctcgttg
     7801 acgggttgcg gcagcgcggc atccagccga tgctcacgct ctatcactgg gacctgccgc
     7861 aggctctcga agaccggggc ggctggacga gccgggagac aagcgaccgg ttcgcggagt
     7921 acgccggggt cgtctacgag gcgctcggcg atacggtgcc gtactggatc acgctgaatg
     7981 agccctgggt cgccgcgtgg ctgggccacg ggtatggcgt gcatgcgccg gggcatgcgg
     8041 ataccgccat ggccctcgcc gccacccacc atctcctcct cggtcatggt ctggccctgc
     8101 aagccatgcg ctccgcggcg ataccaggag acgagaacca gtttgggatc acgctcgtcc
     8161 tccagcctgc gcgtccgtcg cgcgaccgcg aggccgacgc ggatgccgtc cgccgcgtgg
     8221 atggacacgc gaaccgtctc tacctggatc ccctcttcca cggcgagtac ccggacgacc
     8281 tgctctcgca ctaccgcgag cgcggcgtgg aactcccgtc ggtacaggac ggagatatgg
     8341 acgtaatctc cggcgacctc gacttcctcg gcgtcaacta ctacttccgg cacgctgtcc
     8401 gagacgcgcc cgatgaggag ccgaaggcag tgcccttctc cgaactcaac gcccgcgtgg
     8461 tgatcccgca cgacgccgag aagaccgcga tgggctggcc cgtcgagccc gagggcctca
     8521 cgcaggtact cgtgcgcata aaggaggagt acgccgacct gcccatttac ataaccgaga
     8581 atgggcgcgc cgtccacgat tatgtggacc cagagggcgg ggtagatgac gaagagcgca
     8641 tctcctacct ggatgcccac ttccgcgcgg cgcacgaggc gatcggacgc ggcgtggacc
     8701 tcaagggtta catggtctgg tcgttcctgg ataacttcga gtgggccgaa ggttactcca
     8761 agcgcttcgg tatcgtgtac gttgactacg gcacgcagcg gcgcgtccca aaggcgagcg
     8821 cccgctggta ctcggatgta atccggcgca acgggctcga agattaggga gaacggagct
     8881 tgaactatcg tgaactcgac gggaccggga tgcgggtctc ggagatcagc gccggcgtct
     8941 ggaccttcgg gagcggcttc tctttggcgg acctcggcgg cggcacgcgg agaggtctgg
     9001 ccggcatgat gaagcggctt ccagcagtct tccggcgaga agccgtcgaa ggctccgccg
     9061 catgaagcta ctggtaacgg gcggagcggg attcatcgga agcgtggtcg cgtctcaact
     9121 agtcgcggcg gggcacgaga ccgtaatact agacaacctc tcgaaaggac gccgcagtgc
     9181 cgcgccggag ggcgccaggc tgatcgaggc ggacctcctt aacgcggagc ggacacggga
     9241 agttctgggc gagggattcg acggggtgct tcacttcgcg gcgctctcgg tggtgggcga
     9301 gtccgtcgag gagccggagc gctactaccg gaacaacgtc tgcggtacgc tcaacttgct
     9361 ggaggcgatg gtcgcggcgg gcgtaagccg cctcgtcttc tcgtccacgg cggcggtcta
     9421 cggcgagccc gacgaagtcc caatcccgga gaccgccccg gcggttccca ctaacccgta
     9481 cggcgcgtcg aagctggcgg tggaccgtct gatcggagcc gtcgccgaag cccgcgggat
     9541 ctgcgctacg agcctccgct atttcaacgt cgctggggcg agtggtaatt ccggcgaaga
     9601 ccacgggccg gagacccacc tcatccccct ggtcctgaag tcggcggcgg ggaagcggga
     9661 ctcggtgaag atcttcggta ccgactaccc gacgcgcgac ggcacagcta tccgggacta
     9721 tatccatgta gaagacctgg gccgcgcgca ccttctggcg ctggaggacg cgcgtgcggg
     9781 cgagcaccgc gtctacaacc tcggtaacgg caaagggttc tccgttcgcg aagtaataga
     9841 tgtggcccgt cgcgtaacag gcagaccgat tgaggctgtc gaggccccgc gcagggccgg
     9901 agacccgccg gagctagtgg cgtccagcga gaagatccgg gccgaactcg gctggaagcc
     9961 cgagaagccg tcgctggagg ccatgatctc cgacgcctgg acctggatgc aagagcatac
    10021 tggaggttac ggatagcctg cgcgagtgaa cgggcggaga gtcgtccatt cctatcaaga
    10081 gagttcggag aagaaccgga gcttatcgcc agcgccgggc aggaccgacc tttgcaggtg
    10141 agcataaaga ctgcggtggc ggttcgagga gactggattc tacgagtgcc ttgcgcctgt
    10201 ccgcgcgcct cgcctggttg cgagcggcgc ggacggccgc gtgaccatta cggtccgccc
    10261 ggtgtcgccg ggatcgttta gctgctgatt tcgtcgggta cgggaacccg gtatcagatc
    10321 ggaaggagtt ctatcatggc taacgagttg cagggcaaga gagtagccat cctgcttgcc
    10381 cccgtgggta ccgaacaggt cgagttcgtc cagccgaagc aggctgtcga ggacgccggc
    10441 ggcgaggtgg atgtcgtggg tatcgagacc ggcgaggtcc agaccatgaa cagcgacgtg
    10501 aacccgggcg ataccttcgc ggtcgagaag acgttctccg aggtctccgc ggaagattac
    10561 gacggtctga tcgtgccggg cggcactgtt ggggcggaca acctccgcgg cagcgaggag
    10621 gcggtcggtt ttgtccgcgg gttcttcgag caggccaagc ctgtaggcgc gatctgccac
    10681 gggccgtgga cgctggtcga ggcggacgtg gtgaagggca ggaccctcac ctcctaccca
    10741 accctcaaga ccgacatcaa gaacgcgggc ggcaactggg ttgacgagga ggtcgtcgtg
    10801 gatcaggggc tcgtgacgag ccgcaatccc gacgacctgc cggcgttctg cgcacagatc
    10861 gtggaggaga tcgccgaggg cgagcacgcc gagcagaagc gcagcgcctg acggcgagcg
    10921 cctgacatat atcggggcgg tatcgtctcg caacgcaccg ggctccgtca ccgacggggt
    10981 ccggttttcg tctttgctgg cacggaggag gcgctctgag cagggctttt ccagggtcgt
    11041 cggcagggac aatcgaacca gtggcggatg ctgtgaggtt gtcacgcagc gcacaggttt
    11101 atgtgggtta cagacgggta aggaccggcc ttacgagaga tcacctatac ctggaggtat
    11161 ttatggccca gcggatagac ggaagcatag agatagaggc tcccgtagcc aaggtttacg
    11221 actactggaa gactctggag aaccttccca agtttatgag caacatcgag gaggtccgcc
    11281 cgaccggccc ggacaccacg cactggatcg tgcggggtcc tctaggctac aaggccgagt
    11341 tcgacgccaa gaccactcag aacgagccga accaggccat agcctggaac accgagaacg
    11401 gcgacgtgga gacctccggg caggtgcgct tccaggagct taccccgaac cgcacccggg
    11461 tcgaggttca gatgaactac tggaatccgc cgggcggtaa ggtcggcgag gcggcttcca
    11521 gggttacctc cggtcccaag actatcctgg tacaggatct gcagaacttc aaggacatca
    11581 tggaaggtac ggcgaccccg gaagaggtgc agcagcgccc ctccgccgcc gacgtccaca
    11641 gtggcgcgat caccttcctg acctccggga ccggtctact cgtggtcggt ggcagcctcg
    11701 tgctctggct gctacttcgt cgccggggcg gtagctcctc caggaagaag tcgcgcatca
    11761 tcttcgagtt ctaacagcgg tatcgaatat cgggaagaac ccagaccggg gcgtcgtcag
    11821 aaaacgtccc ggtctttttc gtgcctcatg ggatcggcct gtccgacgcg cgttcgcgtc
    11881 tggcgggtcg ctagagacgg tccaccttcg cagccaggat aaagtcgctc tccgtgaggc
    11941 cgtctatcgc gtgcgtgaag acgatcaact ccgcccggcc ccagccgaag ctgatatcgg
    12001 gatgatggcc ctgctcttcg gacaactccc caacccggtt tacgaagtcc agcgcctccc
    12061 ggaaattcgt gaagcgaaac tctctcctca ggtgatgctc cgcgacgacc tcccactcgg
    12121 ggacctggct ggcgagtgca ttcagtacct cgcctccgag cggcggcacc ccgccccggc
    12181 aggggacgca cgtcctgtcg gccagtccgg tcatcggcat ctacctcacg atttaccgtt
    12241 ctgcgggaac ttctcatcgt acaggtccat cgcgtgctgg acggcttcga tggcctcttc
    12301 cggatcggcg aagtcctcca cgttgacctc tttatcttcc agctttttgt agtcctggaa
    12361 gaagctccgc agttcgtcca gccggtgttc gggaagctcc ttgtagtgtt cgtaggagcg
    12421 gaactccggg tcgtcgaggt ggacgcatat gatcttctcg tcgtcctggc cctcgtctac
    12481 catgtgcatc atcccaatgg gctttgctcg caggaggctc agagggaaga ccgtctcctg
    12541 catcaggacc aggacgtcta gcgggtcatc gtcgtctccc agggtgcggg ggataaagcc
    12601 gtagttcgca ggatagtgga tcgatgcgta tagcacgcgg tcgaccagaa ggagtcccgt
    12661 ctccttatcc agctcgtatt tgaccttgga gcccttgggg atctcgatca gggcgttgaa
    12721 ctcccgcgga gcctcttcgc cgatcgggac gtcgtgccag ggatggggca tgccggctag
    12781 ccgtcggcga tcggccggcg actttcaact ccttccagcg acggaacgat actcacgaga
    12841 taaatctacc atcacgctgg gcctcgtgaa acagtcgtgg gaacgatagt gagtaccgga
    12901 ggtgggactc gaacccacac gatcttgcga tcaccggatt ttgagtccgg cgcgtctacc
    12961 attccgccac tccggcgcgg gggcggacgg cattatacca atgtaccaag cgcgcttcgg
    13021 aggcattgca cgggtgcttg acaggaatga tcacatgcgg ccatacttga gccagtagat
    13081 gactctttgc ggaccatgct aaagggatgt aatccacgct ggcccgcgca gcgtacgcgg
    13141 agttgaaaat tatggtacgc aagacaatag gaggcaaggg tgtatagagt acgtagagtg
    13201 gggatgcttg cggcggtcgg cgtcgtctcg gcgttgctga tcgttggctg cggtggcggt
    13261 agcggtggtt cacagggtgg cggtggtggc ggcgacagcg tgaagatcgt cagcgacctg
    13321 ccgctgcagg gagctaaccg cgcccagacc acgacgatga acaacgctat agagctcgcc
    13381 ctcaaggaga ggaaaggcaa ggccggcgac gtcaccgtcg agctggagaa gaaggacgac
    13441 gccacggcgc aggccgggca gtgggacccc agcaagtgcg ccgagaacgc gcagcaggcc
    13501 gcccaggacg actccgtggt cggttggatc ggaccgttca actccggttg cgcggcgacc
    13561 gagatcccga ccctcaacga ggccggtctc ggaatgatca gcccggccaa cacctacatt
    13621 ggtctcacca agaagggcgg cgagcccgac gagccggaca agtactaccc caccggcaag
    13681 cgcaactacg cccgcgttat cgtggccgac gataagcagg gcgcggccgg agcgcagctc
    13741 ctgcagcagg agggcgctaa gtccgtctac atcctcgacg acaagcagac ctacggtaag
    13801 ggcctcgccg accagttcga gaaggccgcc aagaattccg gcatccaggt cctcgggcgt
    13861 gaaggcatag acggcaaggc gtccaactac cgctcgctga tgagcaagat ctcccagtcc
    13921 aacccggacg ctatctactt cggcggcatc atcgagaaca acgccgccca gatcatcaag
    13981 gacaaggtcg gcgccgggat gagcaacgag gacgtgctct tcgttggccc ggacggtatt
    14041 ttcgtggact ccctgatcga ccaggccggc aaggacgcgg agggtatcta cgttaccttc
    14101 ggtggtctgc cggagagcga gctctctgcc aagggtcagg ctttcgtcaa gaagtacagg
    14161 tccacctacg acgatgacat tcagccctac accgcatatg cttacgaggc cgctaacgtg
    14221 atgctcgacg cggtcgagaa ggcctcgaag aaggcaggcg gcgttcccga ccgggaggcc
    14281 gtggtcaagg agatcttcgc cactaaggac tacaacggag tcctcggtaa gtggagcttc
    14341 gacaaggacg gcgacactac gctgaccaag ctctccatcc agaaggtcga gaacggtaag
    14401 ttcaagttcc agcgcaccgt ggacgtcgcg gacctggtgc agtagaaagg acggacggta
    14461 aagctcgggg accggcgcgg tggagatcgt ggccggttcc cgcttctata gaagagggcg
    14521 cggatgacaa cgggtgaacc aacgaagagt aggtccggcg tactgctgga tagtgtccgg
    14581 tcctcgagct ggcaatcgcg catagcggtg gcgctgatcg cggcgctcct catacttctg
    14641 ctggtgcagg cggcgcagga acccggagag ttcgcctctc agctagttat cgggatcacc
    14701 aacggggcga tcatcgcgct catcgcgctc ggctacacgc tggtgtacgg gatcatagag
    14761 ttgatcaact tcgcccatgg cgataacttt atgctcggct ccttcgtcgg cctgacggtg
    14821 ctctcgggca ccttcttcgg gatcggtctc ttcgccccga tcaacgggga atccagcgcg
    14881 ttcctgaagg ttattggcat cggcatcgcg cttgtcgtgg cgatggcggc gtgcgggctt
    14941 atcaacatgg ggatagagcg ggtggcgtac aagcccctgc gcaactcccc gcggctggcc
    15001 gtcctgatca cggccatcgg catgtctttt atcctgcaga acgtgggcct ggtgtggaag
    15061 ggacccaccc aggtcccctt cccggaactc atctccaacg ctaacgtctt caacgagagc
    15121 gtggtcgcct ttcgctggag ggacctcttc gtcatcgtgg tgacggtgcc gctcctggcg
    15181 ggcctctcgt acttcataag gagcaccaag cagggcaagg cgatgcgggc cgtcgcgcag
    15241 gacaaggagg cggcgtcgat gatggggatt aatgtgaacc gtacgatctc catcaccttc
    15301 ctcctcggag gcatcctggc cggggcgtcg ggcgtgatgt tcggcctctt caacggcatc
    15361 accgtcttca gcgtcggatt cacccagggg ctctacgcct tcaccgccgc ggtactgggc
    15421 ggcatcggaa acctgaccgg ggcggtggtc ggcgggttcc tcatcgggat catcggttct
    15481 atgtccgacc agtacatcgg cgtgcggtgg acggccatcg tgatcttcgc ggtcctgatc
    15541 ctggtcctgg tcttccggcc gcgcggcctg ctcggggacc gctcggcggt gcagggcgga
    15601 ggcgagtagg atggcggcgc gtctgaagcg gtactggctg cccatcgtgc ttatcgtggc
    15661 agcgctcctc ttcccgttcc tggacactgc cctggacgcg ggcctgatgt acccggtgat
    15721 aaccatcgcg atctacgtga tgctggccct gggactgaat attgtggtcg gcttcgcggg
    15781 cctcctggac ctcggctttg tggcgttcta cgctctcggg gcctacgtgg tggggtggtt
    15841 cgcctccagc cagttcaatc aggtgagctt ctctttcggc tcgacggccg tcagcctgag
    15901 cggcggctcg ccgcccggca tacacctctc gttctggata ctgctggttc tggccggcgc
    15961 gttcacggcg ctctgcgggg tgatcatcgg ctttccgaca ctgcggctgc gcggcgacta
    16021 cctggctata gtgacactgg ggttcgggga gatcatcccg cgcttcttcc agaacggtga
    16081 cgatctcggc ggcttcaacc tcaccaacgg caccatcggc ataaaggcga tagactcgcc
    16141 gggcatcccg ttcatgccgg actcgctctc gaccttgcag cgcttctctt cgctggacct
    16201 taacccctgg tactacacga tcctgatcct ggtcctcatc acgatctacg tgaacgtacg
    16261 gttgcaggac tcgcggctcg gacgagcctg gatctccgtg cgcgaggatg agacggccgc
    16321 cgccgcgatg ggcatcaacc cggtgacgac gaagctctgg gcctacgcgc tgggagcggt
    16381 cttcggcggg ctcgcgggcg cgttctacgg ggcattcacc aagaacatct tcccggacgc
    16441 cttccagttc aacgtctcca tcttgatcct gtgcatggtc atcctcggcg ggatgggcaa
    16501 catctacggc gtgatcctgg gcgccatcgc cctgcagggt ctgaacttct acctgctgcc
    16561 gaagatcaac gactgggtcc acgctatcgg caacctcgtc ggcagtccgc tcctctcggg
    16621 agccgatatc ccgaagtaca acttcttcct cttcggcgtc atcctcgtcc tgatgatgct
    16681 attgagaccc gaaggaatta tcccgaaccg ccagcgccag gaagagctgc gcggcggcga
    16741 cgaggaacca gccgccacgg agcccgcccg tgtctgagca gaacggtaac accacgagcg
    16801 gcgcgccgaa cggcttggcg aagaacgtct cgaacatcat ggaggcgcgg gggatcacga
    16861 aggtcttcgg tggcctcgtg gcggtcaact cggtcgactt cgacatcccc gaacgctcta
    16921 tcgtcagcct gatcggcccg aacggcgccg ggaagacgac gtttttcaac atggttagcg
    16981 gtctgtacca gcccacctcc ggcacgttct cgctggtggg ccgcgacatt acgcgcctga
    17041 aggcgcacca gcgcgcccag ttgggcatcg gacgcacgtt ccagaacgtc cggctcttcg
    17101 gcacgatgag cgcgcaggac aacgtgctct ccggcatgca cacccgcctg aagggcaaca
    17161 tcctgggctc catcttccgc acgcccgcca cgcgccgcga ggagtcggag gcgagggaga
    17221 aggctcagga gttgctccgg ttcgtcggca ttcaggggcg ggagacgttc gccaagaacc
    17281 tctcctacgg cgatcagcga cgtctggaga tagcgcgggc gctggcctcg gagccgaagc
    17341 tactgctgct cgacgagccg acggcgggga tgaacccgca ggagacggcg aagctgaccg
    17401 acctgatggg ccggttgcgc gaggagcggg ggatagcggt gttgctcatc gagcacgaga
    17461 tgcgggtcgt catgagcatc tcggaccggg taacggtcct ggaccacggc gagaagatct
    17521 ccgagggcgc gcccgccgat gtgcggcagg acccgaaggt gatcgaagcg tacctcggaa
    17581 cggcgaagac gggctaggat gggacagacc ttcgacaaaa taccagacga gttcgccggc
    17641 tggttcgagc agcagccggt gttcttcgtc gccactgctc ccgccgatcc cgagacccac
    17701 gtcaacgtct cgccgcgcgg gctcgacact ttcagggtta taggacagaa ccgcgtcgca
    17761 tggctggatc ttacgggcag cggcgtagag acgatagccc acctcaaggt ggaagaagct
    17821 ggaggacgga tcacgttgat gttctgcgct ttcgaggact caccacggat agttcgcctc
    17881 tacggtcggg gcagggtcca cgagcctggc gacgatgctc acggcgaatt gcgctcccat
    17941 ttccccgatc tgcccggagc gcgggccatc atagacgtga cggtggaccg ggtatcgact
    18001 tcgtgcgggt tcggcgtccc gctgatggag ttcatgggac accgcgacga gctcatcgag
    18061 tccgccaggc gcaagggaga gacgatgatg gaggcctacc gggcgcgaaa gaacgccgag
    18121 agcatcgacg gcctgccggg gttggagtcc gcgaccctcg tggagacgaa ttagcatggc
    18181 gctactcgaa gtagagaaca tgcacagcta ctatggcaac atccacgccc tgcgcggcgt
    18241 ctcgctgacc gtcgaggagg gcgaggtcgt caccctgatc ggctcgaacg gggcgggcaa
    18301 gacgacgacg ctccgctcca tccacggcgt cttgccgccg agggagggca aagtaatctt
    18361 tgacggccag gagatacagg gcgttccggc gcacgacatg atctcgaagg gcatcgccca
    18421 gagcccggag ggacgcaaga tcttccctcg catgaccgtg atggagaacc tggagatggg
    18481 cgcctaccat cgaaacgacc gcgccgggat aaagcaggat atcgaccgtg tcttcgagct
    18541 gttcccacgc cttcaggagc gcattaaaca ggaagccggc acgatgagcg ggggcgagca
    18601 acagatgctc gccatcggcc gcgcactgat gagccggccg aagctgctcc tgctggacga
    18661 accctcgatg ggtctctctc cggtcctcgt ggagcgcatc ttcgacatca tcaaggagat
    18721 caacgcgcag ggcaccacca tactgctcgt cgagcagaac gcgaacatcg ccctggagat
    18781 cgccacccgc ggctacgtcc tcgaaac
//
LOCUS       DASSMB010000128         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_788569, whole genome shotgun sequence.
ACCESSION   DASSMB010000128 DASSMB010000000
VERSION     DASSMB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788569"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="VFJ72_07840"
     CDS             complement(<1..507)
                     /locus_tag="VFJ72_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479408.1"
                     /translation="MKKGTRVLVVEDDRSIARLVELELSHRGLEVRCEGDGLSGLEAV
                     SSFDPAVVILDIMLPGLDGVGVLKRLRAEGNRVPVIMLTARDTLQDKVHSLDLGADDY
                     LTKPFETEELLARLRALVRRYDGEEVLRVADLRINTATREVRRGEGSTEREIDLTNRE
                     YELLEFMAR"
     gene            complement(521..1306)
                     /locus_tag="VFJ72_07845"
     CDS             complement(521..1306)
                     /locus_tag="VFJ72_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7479409.1"
                     /translation="MFERLLDKHRGSFRKISERLSLTAALWLVAGLAVSTGVIALFAK
                     ITEDVAEGESRRFDRAVLLWIHATFPDWLNEPMRLVTTLGYYYVVIPLLAAAVLAFYL
                     RGWKLSAVLLVVSTAGGIVLTTVLKTVFQRARPELFDSGYTASFFSFPSGHATVAVGF
                     YGMLTLILAYHLRGLARWAVAAGGVLLVLTIGFSRLYLGVHYPTDVLAGFLASPLWVI
                     TVGAVYVLWLALRGRTARENVDRENVDREEQTEEGISGARKDT"
     gene            1334..2164
                     /locus_tag="VFJ72_07850"
     CDS             1334..2164
                     /locus_tag="VFJ72_07850"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET7479410.1"
                     /translation="MRVMTFNIRGFRRDAGTPNAWKKRVGSNVEVISRCDPDVIGLQE
                     CQPGNLKAYRRRLPHYGRIRGPRYGNRPPHDFNAILFHESRLEVVESGGFWLSETPEE
                     YSRSWETRVARSANWVLFRSLEDGEASGATFLHLNTHLDHRSKPARVEGSRLVVRKIA
                     ELRASLGDPPVIVTGDFNAVPGSAPYRNFVAAGFTDAYLAAGNEEAEDVHTFHNFRGP
                     EFVPRRGRSPKRLDWVMFHDSLERVRVESCRIARYRDEGSGTFPSDHYPVVADLSFGK
                     "
     gene            2235..>3815
                     /gene="polX"
                     /locus_tag="VFJ72_07855"
     CDS             2235..>3815
                     /gene="polX"
                     /locus_tag="VFJ72_07855"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HET7479411.1"
                     /translation="MQNNDIVRALATIATLMEIKDESHYRVLAYQRAAETVAALGRPV
                     REVEDLKKLPHIGGATAEVIQDLAEDRMPQLLTELTEEIPAGLVEVTRLPNVGPRTAG
                     RLWRELDVKSVEDVAELEEGQLAALKGFGKKSEERILRAARTYNATERRMLLDEATAL
                     GEHLLEFVRSHPATKRAETAGSLRRMKETIGDLDIVAASTDPESLANDFAGAPFVDEV
                     VAHGNTKVSIVCGGADVDLRIVAPGAYGSLLHHFTGSQAHNVVLRERAVKMGINISEY
                     GLAQEGTKDYKPVADEEELYERLGLPYIPPELREDAGELEAAESGTLPDLIETKDVRG
                     DLHVHTNYSDGRGTIESMAEAAIALGYEYLVFCDHSQSLKVANGLSPERLKEKMEAVR
                     KADEKYGEIRLLCGSEVDILKDGTLDYEDALLAELDFVVAAVHTSFGVGEKAMTERII
                     RAINNPHVRTVAHPTGRILNRREPYEVDVSRLIKEAAATNTALELNSYVDRLDLAVPY
                     VREAMNAGVKITVDTDAHD"
BASE COUNT          756 a         1292 c         1194 g          573 t
ORIGIN      
        1 gcgcgccatg aactcaagca gctcatattc gcggtttgtc aggtctatct cgcgctcggt
       61 agagccttca ccgcgccgga cctcgcgcgt agcggtgttt atccgcaggt ccgcgacacg
      121 cagcacctcc tcgccgtcgt aacggcgcac gagggcgcgc agccgggcca agagctcctc
      181 agtctcgaaa ggcttggtga ggtagtcgtc agcaccgaga tccaggctgt gcaccttgtc
      241 ctgaagggtg tcgcgagcgg tcaacatgat caccggaacc cggttgcctt ccgctcgaag
      301 ccgcttcagc accccgacac cgtcgagccc cggcaacatt atgtcgagga tcaccaccgc
      361 cggatcgaag ctcgacaccg cctccaggcc ggagagtccg tcgccctcgc accgtacctc
      421 cagaccgcgg tgcgagagct ctagctccac gagccgggct atggagcggt cgtcctcgac
      481 caccaaaacg cgagtcccct tcttcaacgg atacctctct tcaagtatcc ttccgggcgc
      541 cggagatccc ctcttcggtc tgctcctcgc ggtccacgtt ctcgcggtcc acgttctcgc
      601 gggcggttct tccgcgcagg gcgagccaca ggacatagac ggctccgacc gtgatcaccc
      661 acagcggcga cgccaggaac ccggccagca catccgtcgg atagtggacg ccgaggtaga
      721 gccgcgagaa ccctatggtg aggaccagca gcaccccgcc ggcggccacg gcccagcgag
      781 cgagaccccg caggtggtag gccaggatca gggtcagcat cccatagaag cccacggcca
      841 ccgtggcgtg tccgctcggg aatgaaaaga aagacgccgt gtagcccgag tcgaagagct
      901 ccgggcgcgc tcgctggaat acggtcttga gcacggtggt gagcacgatg cccccggcgg
      961 tcgagacgac aagcaacacg gccgagagct tccaacccct gaggtagaag gcgaggaccg
     1021 cggcggcaag gagcgggatg actacgtagt agtagccgag ggtggttacg aggcgcatcg
     1081 gctcgttcag ccagtccggg aaggtcgcgt ggatccagag tagcaccgcc cggtcgaagc
     1141 gccggctctc cccctcggcc acgtcctcgg ttatcttggc gaacagcgcg atgacgccgg
     1201 tggagaccgc gagaccggcg accagccaca gggccgcggt cagcgacagc cgctccgaga
     1261 tcttgcggaa ggacccgcga tgtttatcca gcagacgctc gaacatgagg gatattctaa
     1321 actgcatacg gccatgaggg tgatgacgtt caacatccgg gggttccggc gcgacgccgg
     1381 cacacccaac gcctggaaga agcgcgtggg gtcgaacgtg gaggttatct cgcgttgcga
     1441 cccggacgtg atcggccttc aggagtgcca gcccggcaac ctgaaggcgt accggcgccg
     1501 tctcccgcac tacggtcgga tacgcggtcc caggtacggc aaccgtccgc ctcacgactt
     1561 caacgctatc ctgttccacg agtcgcgcct cgaagtcgtc gagtccggcg ggttctggct
     1621 cagcgagacc cccgaagagt actcccggag ctgggagact cgggtggccc gctccgccaa
     1681 ctgggtgctc ttcagaagcc tcgaagacgg cgaagcctcc ggcgcgacct tccttcacct
     1741 caacacgcac ctcgatcacc gcagcaagcc cgcccgcgtg gagggcagca ggctcgtggt
     1801 acggaaaata gccgaactcc gagctagtct cggcgacccg ccggtcatcg ttaccgggga
     1861 cttcaacgcc gtccccggct ccgcaccgta ccgcaacttc gtggcggcgg gctttaccga
     1921 cgcttacctc gccgccggca atgaggaagc ggaggacgta cacaccttcc acaacttcag
     1981 ggggccggag ttcgtacctc ggcgcggccg gagcccaaag cgtctggatt gggtcatgtt
     2041 ccacgactcc ctggagcgcg tgcgggtgga gtcctgccgg atagcgcgct accgggacga
     2101 aggttccggc acgttcccga gtgaccacta ccccgttgtc gcggacctct cgttcgggaa
     2161 gtaggctctg ggtagaagct cgcggccacg tataatctgc tggcgagaga tcggccacca
     2221 ggaaggtcca cgagttgcag aacaatgaca ttgtccgggc gctggcgacc atcgcgacgc
     2281 tcatggagat aaaagacgag agccactacc gggtgctcgc ctaccagcgc gccgccgaga
     2341 ccgttgccgc cctgggacgg ccggtacggg aggtcgagga cttaaagaaa ctgccgcaca
     2401 tcggcggggc gacggcggag gtgatccagg acctggccga agaccggatg ccccagctcc
     2461 tgaccgaact aaccgaggag atcccagccg gactcgtcga agtcacccgc ctcccgaacg
     2521 ttggtccgcg caccgccggg cggctctggc gcgagcttga cgtgaagagc gtcgaggatg
     2581 tcgcggagct tgaagaggga caactcgccg ccctcaaggg ctttggcaag aagagcgagg
     2641 agcgcatcct gcgtgcagcc cgcacctaca atgccaccga gcgccgcatg ctccttgacg
     2701 aggcgacggc tctcggagaa catctgctgg agttcgtccg ctcccatccc gctacgaaga
     2761 gagccgagac cgccggttcc ctgcgccgca tgaaggagac catcggcgac ctggatatcg
     2821 tcgccgccag caccgacccc gagtccctcg ccaacgactt cgccggagcg ccgttcgtgg
     2881 atgaggtcgt ggcgcatggc aacacgaagg tctccatcgt gtgcggcggc gcggacgtgg
     2941 acctccgcat cgtcgcgccg ggggcttatg gatccctcct tcaccacttc actggcagcc
     3001 aggcacacaa cgtcgtgctg cgcgaacgag ccgtgaagat gggcatcaac atctccgagt
     3061 acggtctggc gcaggaaggg acgaaagatt acaagccggt cgcggacgag gaagagttgt
     3121 acgaacgcct cggcctcccc tacatcccgc ccgagctgcg agaggacgcc ggggaactgg
     3181 aggctgccga gagcggcaca ctgccggacc tcatcgagac caaagacgtc cgcggcgacc
     3241 tgcacgtcca caccaactac tccgacggcc gcggcaccat cgagagcatg gccgaggccg
     3301 cgatagctct aggctacgag tacctggtct tctgcgacca ttcccagagc ctcaaggtag
     3361 ccaacggcct ctcccccgag cgcctgaagg agaagatgga ggcggtgcga aaggcggacg
     3421 agaagtacgg agagatccgc ctgctctgcg gctccgaggt agacatcctc aaggacggaa
     3481 ccctcgacta cgaggacgcc ctgctagccg agctggactt cgtcgtcgcc gccgtccaca
     3541 cgtcgttcgg ggtcggcgag aaggccatga ccgagcgtat catccgcgcc atcaacaacc
     3601 cgcacgtccg caccgtcgca cacccgaccg gccgcatcct caaccgccgc gaaccatacg
     3661 aggtagatgt atcgcgcctc atcaaagaag ccgccgccac gaacactgcc ctggaactca
     3721 actcctacgt ggaccgactc gacctcgccg tcccctacgt ccgcgaggcg atgaacgccg
     3781 gcgtgaagat cacggtagac accgacgccc acgac
//
LOCUS       DASSMB010000129         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1549638, whole genome shotgun sequence.
ACCESSION   DASSMB010000129 DASSMB010000000
VERSION     DASSMB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549638"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFJ72_07860"
     CDS             <1..363
                     /locus_tag="VFJ72_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET7479412.1"
                     /translation="GSGGALAMCAADYVGMLENSYLSVIAPEACASIIFRDPARAPEA
                     AEAMKLTAKDLKEHRIIDEILPEPLGGAHNEPQATIETVGEALAVTLSELSGADPQTL
                     QKTRYERYRRIGAHISSG"
     gene            complement(405..1274)
                     /locus_tag="VFJ72_07865"
     CDS             complement(405..1274)
                     /locus_tag="VFJ72_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7479413.1"
                     /translation="MFEGFDNIRIETREAEINIVRGGSGPPVLLLHGYPQTHAMWDRV
                     ATLLAGSFTVVAADLRGYGDSSKPEGDPEHARYSKREMAADQVEVMRALGFETFAVVG
                     HDRGARVGHRMALDHPERVTRLAALDIVPTRHIFETVGKELATVYYHWFFLIQPADFP
                     ERLIGPDPAYYLRSALGRYGADLSIFAPEALAEYERCFDRRTIHASCEDYRAAASIDL
                     VHDEADRGNKLRCPLLLLWGARGAMHGLYDVEAVWREYAEDVRGRPLDAGHYLAEERP
                     EETARELLAFLQG"
     gene            complement(1280..2329)
                     /locus_tag="VFJ72_07870"
     CDS             complement(1280..2329)
                     /locus_tag="VFJ72_07870"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET7479414.1"
                     /translation="MAEVKKVGMLTGGGDAPGLNGVIRAVTMRCIEEYGYEVVGLKRG
                     WKSLLSPESDTVMPLGVDDVRYILQEGGTILGSSRTNPYKKEGDPEKVVKQMAEFGVD
                     ALVAIGGDDTLGVAKRLYDDFDVQVIGCPKTIDNDLSATDTTFGFDTAVSIATDAIDK
                     LRTTAKSHERVVVVEIMGRHAGWITWGAGVASAANVTLIPEVEPDLDEIADIFKKRAE
                     NGEKWGLVAVSEGVVLSEDYITQNAERDEFGHVRLGGIAETLAKEIEERTGIETRHVV
                     LGHLQRGGTPTAFDRILSTRYGLRAAEAVKNGEWGQMVALRGNDIVTVSLAEATGETK
                     TVPDDFYQAARTFFG"
     gene            complement(2414..2974)
                     /locus_tag="VFJ72_07875"
     CDS             complement(2414..2974)
                     /locus_tag="VFJ72_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479415.1"
                     /translation="MSCRVLRGKQGDFWFTLHSWAQVLDMANLNGWIPAKTRPAEWPV
                     DNEEYNRMVAQSGGRVEFFALEDEEGEEVFTGYLYPPGRWHGGYLTAERQVVTAEDAR
                     HMADALEEVLEEVPEQDQYDPFVPENDRDRIHQVSEDDVTFDEWFSGPARKHLQAFIE
                     FCRAGEFIVLDEYKAEEKGVEQDLAQ"
     gene            complement(3100..>3442)
                     /locus_tag="VFJ72_07880"
     CDS             complement(3100..>3442)
                     /locus_tag="VFJ72_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7479416.1"
                     /translation="GSEQPAHPNRGFGGKEQVRKNWSGMFESFPDFRAELLRHTSDGE
                     TAWSEWDWRATGLHMAGVIVMGVKDGRMLWARLYMEPVEEAGQDIDEAMRTITSTGRR
                     PDEDSEEASTT"
BASE COUNT          522 a         1190 c         1090 g          640 t
ORIGIN      
        1 ggctccggcg gagcgctcgc catgtgcgcc gccgactacg tggggatgct ggagaactcg
       61 tacctctccg tcatagcgcc ggaggcgtgc gcctcgatca tcttccgcga ccccgcccgc
      121 gcccccgagg ccgccgaggc catgaagctc acggcgaaag acctcaagga gcaccggata
      181 atagacgaga tactgcccga acccctcggc ggcgctcaca acgagccgca ggcaaccata
      241 gagaccgttg gcgaggcgct cgcggtaacg ctctccgagc tatcgggcgc cgacccgcag
      301 acgctccaga agacgcgcta cgagcgctac cgccgcatcg gagcccacat ctccagcggc
      361 tgacggccag gatagccgga gacggtcatc ccgcgcgtag cgcactaccc ctggaggaag
      421 gctagcaact cccgcgcggt ctcctcggga cgctcctcgg cgaggtagtg gcccgcatcg
      481 agcggtctgc cgcgcacgtc ttccgcgtac tcgcgccaga cggcctccac gtcgtagagg
      541 ccgtgcatcg cgccccgcgc tccccagagt agcaagagcg ggcatcgcag cttgttcccg
      601 cggtctgcct cgtcgtggac caggtctatg ctggcggcgg cccggtagtc ctcgcagctc
      661 gcgtggatgg tgcgccggtc gaagcagcgc tcgtactcgg ccagggcctc gggcgcgaag
      721 atgctcaggt ccgcgccgta ccggccgagc gccgaacgga ggtagtaggc tgggtccggg
      781 ccgataaggc gctcggggaa gtccgctggt tggatcagga agaaccagtg gtagtagacg
      841 gtcgccagct ccttgcccac ggtctcgaag atgtggcggg ttggcacgat atcgagcgcg
      901 gcgagccggg tcaccctttc cgggtggtcg agcgccatcc ggtgtccgac ccgagccccg
      961 cggtcgtggc cgacgaccgc gaaggtctcg aagccgagag cccgcatgac ctcgacctga
     1021 tccgcagcca tctcgcgttt cgagtagcgg gcgtgttcgg ggtctccttc cggtttggag
     1081 ctgtcgccgt agccgcgcag gtccgccgcc acgaccgtaa agctcccggc cagaagcgtt
     1141 gctacacggt cccacatcgc gtgggtctgc gggtagccgt ggaggagcaa gaccggcggc
     1201 cccgagcctc cgcggacgat gttgatctcc gcttcgcgag tctctatacg aatgttatcg
     1261 aagccttcga acacggcttt tagccgaaga aggtgcgggc ggcctggtag aagtcgtccg
     1321 ggacggtctt cgtctcgccg gtcgcctcgg ccagggagac ggtgacgatg tcgttgccgc
     1381 gcagggcgac catctggccc cactcgccgt tcttgacggc ttcggcggcg cgcaggccgt
     1441 agcgggtgga gaggatgcgg tcgaaggcgg tcggggtgcc gccgcgttgc aggtgtccca
     1501 ggacgacgtg ccgggtctcg atgccggtgc gctcctcgat ctccttcgcc agcgtctcgg
     1561 cgatgccgcc gaggcggacg tgcccaaact cgtcgcgctc ggcgttctgg gtgatgtagt
     1621 cctcggagag cacgacgccc tcactcacgg cgacgaggcc ccacttttca ccgttctcgg
     1681 cgcgcttctt gaagatatcg gcgatctcgt ccaggtccgg ctcgacctcg gggatgaggg
     1741 taacgttcgc ggctgaagcg acgcccgcgc cccacgtgat ccaaccggcg tgccggccca
     1801 tgatctcgac caccacgacc cgctcgtggc tcttggccgt cgtgcggagc ttgtctatgg
     1861 cgtccgtggc tatggagacc gccgtgtcga acccgaaggt cgtgtccgtc gcggagaggt
     1921 cgttgtctat cgtcttcgga cagccgatga cctggacatc gaagtcgtcg tagaggcgct
     1981 tggcgacgcc cagggtgtca tcgccgccga ttgcgacgag cgcgtccacg ccgaactccg
     2041 ccatctgctt cacgaccttc tcagggtcgc cttctttctt gtaggggttc gtgcgggatg
     2101 aaccgaggat ggtgccgcct tcttgaagga tgtagcgcac gtcgtccact cctaggggca
     2161 tcacggtgtc cgactcgggc gagaggagag acttccagcc gcgtttcagg ccgacgacct
     2221 cgtagccgta ttcttcgatg caacgcatgg ttacggcccg gatcacaccg ttcagccccg
     2281 gcgcgtcgcc gccgccggtc agcatgccga ccttctttac ctcggccatg ttccgtcctc
     2341 cttcactccg ttcgacccac cagaagatca gtctagcaaa ctgccgcgcc gcgcttgtcc
     2401 tgggacctcc gggctactgc gccaggtcct gctccacgcc cttctcctcg gccttgtact
     2461 cgtcgaggac gatgaactct cccgcccggc agaactctat gaacgcctgg aggtgtttcc
     2521 tggccgggcc gctgaaccac tcgtcgaagg tcacgtcgtc ctcggatacc tggtggatgc
     2581 gatcccggtc gttctccggg acgaacggat cgtactggtc ttgctcgggg acttcttcca
     2641 ggacttcttc cagggcgtcg gccatgtgcc gggcgtcttc ggcggtcacg acctggcgct
     2701 cggcggtcag gtagccgccg tgccagcgtc cggggggata gaggtagccg gtgaagacct
     2761 cttcgccttc ctcgtcctcc agcgcgaaga actcgaccct tccgccgctc tgggccacca
     2821 tgcggttgta ctcctcgtta tccaccggcc actcggcggg tctggtcttc gcggggatcc
     2881 aaccattcaa gttcgccatg tcgagcacct gcgcccaact gtggagcgta aaccagaagt
     2941 cgccctgttt accccgaagg accctgcaac tcatccaccc tcctcgatct ctcataccct
     3001 ggcccttgcc cggcctccgt ccgccggaac tatgcacgga taacgcctga aagtcgagtg
     3061 ctcctggatc taggctcgcc gaccagcttt cagtagccgc taagtcgtgc tggcttcctc
     3121 cgagtcttcg tccggtcggc ggccggtgct ggttatggtg cgcatggcct cgtctatgtc
     3181 ctgacccgcc tcttcgacgg gctccatgta gagccgggcc cacagcatgc ggccgtcctt
     3241 cacgcccatg acgatcacac cggccatgtg caggcccgtg gcgcgccagt cccactcgct
     3301 ccacgccgtc tccccgtcgc tcgtgtgccg gagcaactcc gccctgaagt ccgggaagct
     3361 ctcgaacatg cccgaccagt tcttgcggac ctgctccttg cccccgaagc cccggttcgg
     3421 gtgggcgggc tgctcgctcc ca
//
LOCUS       DASSMB010000130        19071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_914809, whole genome shotgun sequence.
ACCESSION   DASSMB010000130 DASSMB010000000
VERSION     DASSMB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 19071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19071
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914809"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFJ72_07885"
     CDS             complement(<1..651)
                     /locus_tag="VFJ72_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479417.1"
                     /translation="MKTRRREMGRVKYSPDAAEMEVSRKERLEERRRSRRRAFYSLLI
                     AAGLVLGVLVLGERVLSTGAGTVASAVTPVVLPAPEPLVNDALIEARARSAGETARRA
                     AEEKLAKQTTEEKAAAHRETAKRAAGEDRTSAGQASAGQTIEEQPSSRQPPVAQSESS
                     PSGTASPSGAPAPPSTAMTLTVPAMGKSGEPIGEGVDEGTLYNGPGHAPGTGYPWIA"
     gene            927..1208
                     /locus_tag="VFJ72_07890"
     CDS             927..1208
                     /locus_tag="VFJ72_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479418.1"
                     /translation="MVAFGGGRKPLLVPDPGQKIALISEDGSTREGFRAVSGPLTSDT
                     GETIVQVVTEREYQDARLDGRPPVFLSWPANRVTFPVPRYWWLKRRFSR"
     gene            complement(1258..1331)
                     /locus_tag="VFJ72_07895"
     tRNA            complement(1258..1331)
                     /locus_tag="VFJ72_07895"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1295..1297),aa:Ile,seq:gat)
     gene            1492..1992
                     /locus_tag="VFJ72_07900"
     CDS             1492..1992
                     /locus_tag="VFJ72_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET7479419.1"
                     /translation="MSDNGLTRMSIYGINMDLFTSSPIVILKVEEENRYLPIWIGQSE
                     ARSILMKLQNQEFSRPLTHDLAVNLVSELGGSMERVIVTRLQDSTFFATISVDIGGRT
                     VEIDSRPSDAIALAVRSGAEIFAANEVIEEAGVVFEEAIEDAPEEEVVDKFKDWMNRV
                     SPEDFK"
     gene            complement(2019..2285)
                     /locus_tag="VFJ72_07905"
     CDS             complement(2019..2285)
                     /locus_tag="VFJ72_07905"
                     /inference="COORDINATES: protein motif:HMM:NF039182.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HET7479420.1"
                     /translation="MTGEVLDKFSALISTALGLVAALAWNTAIQNLFNKIFGQAGAAL
                     IGQFIYAVLITLVVIFATIAVSRAAERAKKAEEERQGLFGRSKD"
     gene            complement(2275..2559)
                     /locus_tag="VFJ72_07910"
     CDS             complement(2275..2559)
                     /locus_tag="VFJ72_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479421.1"
                     /translation="MDLLMVRDRATGRFVYTERLERRFGETPWAYVRRSVRREGQIRT
                     RFNQSTMEVIVGWGTGSVEEFLSAYPEYRVEESNSSGTEDDKLKGGTVDR"
     gene            complement(2562..3641)
                     /locus_tag="VFJ72_07915"
     CDS             complement(2562..3641)
                     /locus_tag="VFJ72_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479422.1"
                     /translation="MAEHVADYEWRGEGDEAEVVLYAADSSAFERALFAARLPGVESP
                     VYAAASESGFGWVAASTESVAPDLASVPVRGMLLTSDEPVGGLGVRPEDVAPMILRKL
                     AEVGIPALGAAGVRRVCGVGARAAAEDELIEEEDLPFFGSRSGEPDSLDRRAITTGSR
                     SWEERLGSEIDVYVVGEPFDAERVEEAGIGPGALAIRVRAGSGDLGRLAVSAHRERIL
                     RRVWSGEFELDQDLPVAPVGSEEAVDLLAALRSAENFADGRAALLLYALRRALRDMGS
                     DLRIRASWGVGGIEEREGSFVHRRGLATLGEGEVLVAGSSVAAGTGKMWGSVPPFGVE
                     EDEGRWPWEEAGLLERLAGLKAPGG"
     gene            complement(3653..5395)
                     /locus_tag="VFJ72_07920"
     CDS             complement(3653..5395)
                     /locus_tag="VFJ72_07920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7479423.1"
                     /translation="MFSKVLIANRGEISVRIIRALREMGIESVAVYSDVDRDSLHAAL
                     ADEAYHIGPAPAPESYLNIAKLVETAKRSGAEAVHPGYGFLAESAPFARAVTEAGLVW
                     IGPHPEAIEAMGSKVESRRIMMKAGVPMVPGTKDSISSAKEVLVFGEEHGYPVAVKAS
                     AGGGGKGFAVAQDDAAAEGAFERASREGEAYFGDGSVYVEKYLPGPRHVEIQVVRDKH
                     GNAVHLGERDCSIQRRHQKLLEECPSPALTPAMRQEMGHAAVEAAEAVGYDNVGTVEF
                     LVQDHEFYFLEMNTRVQVEHPVTEEVTGIDIVKTGIRIAAGEPLPFAQEDVSWRGHAI
                     EARLNAEDAANDFAPSPGGVTAYEEPGGPGVRVDSSLSGPGVVPESYDPLFAKLIVRG
                     TDREDALTKLRRALSEFRVEGIATTLPFFRAILDDEVFISGDYTTGFIAERMDGLKIE
                     PASLGKIEARGEASEKRAREVEVEVNGRLFRVKVFGDEVANGGGGPPRRKTGETRRAS
                     VSQGAVAAPMQGTIVKVLVEEGQEVAADEPVCVLEAMKMESEIRAPKSGTVSEVRVEA
                     GQTVRSGDPLIVIE"
     gene            complement(5396..5620)
                     /locus_tag="VFJ72_07925"
     CDS             complement(5396..5620)
                     /locus_tag="VFJ72_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479424.1"
                     /translation="MTETRRIYTEPVPTPEEAAAIMAALEALREAEPEKGSSLKRSRW
                     KLAGLLGHQVPPGMTLEGSLWSYSSWEGIV"
     gene            complement(5617..5940)
                     /locus_tag="VFJ72_07930"
     CDS             complement(5617..5940)
                     /locus_tag="VFJ72_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479425.1"
                     /translation="MILLRAWLLHVVLVATLFSAELLFGAPDWSSGNAGTFTFGLLFS
                     CPLGFWSSLTVMIEGGSENASRDRMSLMLSTLGLQLGGFVVGVLVGLALTVYLISPLI
                     RYVEG"
     gene            complement(6117..6677)
                     /locus_tag="VFJ72_07935"
     CDS             complement(6117..6677)
                     /locus_tag="VFJ72_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479426.1"
                     /translation="MNVIFDRWFKTWPERAGVALISALAIALALLGLMGSDTGFWVLV
                     VLLGVLIGHLSAYSVSMPFRGRFVRRFDSWADVVAFVSGFALGLMLLLIGAFFVGSPS
                     FFSDGGGMSGALVYGLALGVPQGLRSGLVPRPQPGPEQVYQSRRAILQGILIVAGALF
                     TILALAFGLYALIQYILGPLIRYFAG"
     gene            complement(6697..8271)
                     /locus_tag="VFJ72_07940"
     CDS             complement(6697..8271)
                     /locus_tag="VFJ72_07940"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET7479427.1"
                     /translation="MQGERLGENTTAGKIEGLEKLREAAAHPAPEKAVERQKEKGKLT
                     ARERIELLLDEGTFVELDRYRVHRSYNFGLEENRPLGDGVITGYGEVSGRKVCVFSQD
                     FTVFGGSLGEVYAQKICKVMDLALNTGCPIVGINDSGGARIQEGVVSLAGYADIFHRN
                     VLASGVIPQISVVMGPCAGGAVYSPAITDFIFMVEETSHMFITGPDVIKSVTGEDVTQ
                     EDLGGAATHNTKSGVAHFSAPDEESCLDDVRYLLSFLPENNLESAPYYKPADDPERMD
                     EDLASLVPDSANRPYDIREAVVSIMDDGEFFEVQEGWAQNLVVGFARLDGHAVGVVGN
                     QPAVLAGTLDIDASVKGARFVRFCDAFNIPLVTFVDVPGFMPGTDQEWGGIIRHGAKL
                     LYAFSEATVPKMTVITRKAYGGAYDVMNSKHIGADVNVSWPTGEIAVMGASAAVGIIF
                     RRQIAEAEDPEAERERLIEGYEEQFNNPMVAAEMGFVDDVIDPRETRPYLIKALQMIR
                     TKRPERPPRKHGNIPL"
     gene            complement(8347..8697)
                     /locus_tag="VFJ72_07945"
     CDS             complement(8347..8697)
                     /locus_tag="VFJ72_07945"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HET7479428.1"
                     /translation="MRFKIDENLPVELAEELRAAGHEAATVDDQRLVGASDRHLSEVC
                     KTEGRVFVTLDLDFADIQTYPPDEHPGLIVLRLARQDKVHVLDVFRRTLKAVEHEPLE
                     GRLWIVEEKRIRIR"
     gene            complement(8706..8933)
                     /locus_tag="VFJ72_07950"
     CDS             complement(8706..8933)
                     /locus_tag="VFJ72_07950"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET7479429.1"
                     /translation="MDWREHITVDPDVCHGKPCIKGTRVMVSVVLDNLAAGQTPEEIA
                     ESYPSVGREDIQAAIAYAADLARERVVPLPA"
     gene            9172..10278
                     /locus_tag="VFJ72_07955"
     CDS             9172..10278
                     /locus_tag="VFJ72_07955"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HET7479430.1"
                     /translation="MQVFEKLSEYRYEQLVFCHDKSTGLRAIIAIHDTTLGPALGGCR
                     MYPYATEDEAVVDALRLARGMTYKAAASGLNLGGGKSVVIGDPHTDKSEALFRSLGRY
                     VETLGGRYIVAEDVGISTSDISYIHVETSHVVGMDVTYGGSGDPSPFTALGVMQGMRA
                     CAEEVFGTDSLESRTVAVQGLGHVGYHLCRLLHEEGARLIVTDIEREAVERVAREFGA
                     KVVEPDEIRTMPCDVFAPCAMGAVLNDATIPDLRCEIVAGSANNVLLEARHGEALADR
                     GILYAPDYVINAGGLINVADELEGYNERRATRRVMRIKDRIRQIIAISKRDGVPTNVA
                     ADTLALERIAAISSMERLHTGHPYGQLQRRRETI"
     gene            complement(10330..10890)
                     /locus_tag="VFJ72_07960"
     CDS             complement(10330..10890)
                     /locus_tag="VFJ72_07960"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET7479431.1"
                     /translation="MRAAILVIDMLEDFVYGGLRCERADGILEPLGRLLGRARAAGTP
                     VIFSNDAHLPEIDHEFEVWGPHAVAGTPGAEVIGALAPEDEDYIVPKRRYSGFYGTDL
                     EMLLGELGVDTVVLTGLHTNICVRHTAADAFYRGYKVVVPADAVEAFTEEDQTGGLEY
                     LEKVYGAELTSVDALLERIAGAAVEG"
     regulatory      11099..11209
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11289..12656
                     /gene="thiC"
                     /locus_tag="VFJ72_07965"
     CDS             11289..12656
                     /gene="thiC"
                     /locus_tag="VFJ72_07965"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF006763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET7479432.1"
                     /translation="MLEQRGTRRPGEAGESVTQLRYAREGVITEEMRFVAERERMEAE
                     VIREEVARGRMIIPANVNHFSLEPMCIGKAARVKINANIGNSQTTSEIDAEIEKLRTS
                     VKYGADTVMDLSSGGDIPRIRRAIIEASPVPIGTVPIYEVISRVDSPEEMTGDLFLEV
                     IQEQAEQGVDYMTIHAGVLLQHLHLVQSRITGIVSRGGGLLAKWMVHHMKQNPLYERF
                     DEILDICRRYDVSISLGDGLRPGSVADASDDAQFAELRTLGKLTERAWAKDVQVMIEG
                     PGHIPLDQLEMNVKMQQEICHEAPFYTLGPLVTDIAPGYDHITSAIGAAVIGWHGTSM
                     LCYVTPKEHLGLPNAEDVKQGMIAYRIAAHAADIARGRPGAREWDDELSRARYAFDWR
                     RQFDLALDPDTARNMHDETLPDDYFKTAEFCSMCGPKFCPMHNFRDVDWGALKKATDR
                     LPAGG"
     regulatory      12779..12869
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            12856..13617
                     /gene="thiE"
                     /locus_tag="VFJ72_07970"
     CDS             12856..13617
                     /gene="thiE"
                     /locus_tag="VFJ72_07970"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7479433.1"
                     /translation="MDTGNETSVAAAPPGARRLFCGAAVGPKGSVLARDLDIHLITDR
                     KRARGDLPEVVRAALLGGVDFVQVREKTGPARNLYETTLQILPHARKAGAGVLVNDRV
                     DVALATGADGVHLAGKSLPPRIARKLIGELLLGVSVHGLAEAREAVSGGADYVTFGHV
                     YPTSSKPGLPPRGLIELAEIVESVNIPVLAVGGIEPSNALEVLKTGVSGISVISTILA
                     APDPEKATRELRGILDVSGLRPRRRFPKQRMKGAR"
     gene            13614..14603
                     /gene="thiS"
                     /locus_tag="VFJ72_07975"
     CDS             13614..14603
                     /gene="thiS"
                     /locus_tag="VFJ72_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET7479434.1"
                     /translation="MNVTLNRETVETENPEPTISILLAERGISEEGIVVAVNGDVIRR
                     GEWEEHVVRSGDEVEIVHAVAGGSGEDELEIAGEVFSSRLFLGTGKYSDAETMNAALA
                     ASETGMVTVAIRYMDLSGDGASSILDQLDLSRYRLLPNTAGATTAEQAIRMARLAREA
                     TGTNWLKLEVIGDQDTLWPDVAATVEATKVLVEEGFVVLPYTSPDIVAAVRLEEAGAA
                     TVMPLGSPIGSGQGMVDWAGIRRVIERISVPVVVDAGIGVPSDAATAMELGADAVLVN
                     TAIARADDPVRMAEAMKLGTRAGRLAYLAGRMPISGTAHPSSPTRGVPAVSAS"
     gene            14605..15732
                     /gene="thiO"
                     /locus_tag="VFJ72_07980"
     CDS             14605..15732
                     /gene="thiO"
                     /locus_tag="VFJ72_07980"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HET7479435.1"
                     /translation="MKASVDVAVIGGGVIGCSVAYHAARRGASVALFEAGQIGSGASG
                     AAAGMLAAQAEAREPGPFLDLLLESREMHRAFGEELYEETSLDPEYVWAGTLRAALDE
                     GPVEELRARHLWQRRHGLSARWMSGDEARELEPALSSRTVAALYLPEDGQVNSPRLVR
                     SLSLAAARSGARVEEFTKVCGLVVEGSTITGVRTSAGETAAGAVVLAGGASSASLVSD
                     LGVEVPVHPVKGEILAAVPPSLPIKANVWDERCYMVPKRDGRVVIGATEEPGVRDPRP
                     TLGGVAALSGAAVRLVPEISAATFASAWGGLRPGTPDNLPVLGPVDGVEGLMLATGHY
                     RNGVLLAPLTGEVISALALGETPTTDISPFSAHRFTRAAHT"
     gene            15729..16178
                     /locus_tag="VFJ72_07985"
     CDS             15729..16178
                     /locus_tag="VFJ72_07985"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET7479436.1"
                     /translation="MIELPADLLVTMVSHARREAPREVCGWLAGSDGGIIRVLPVPNA
                     AEDPLTRFLMEPVAQVRAMRRVAKMGLDLIGTYHSHPRTPATPSARDRQLAAYSEMAH
                     LILSLSNPEPEIGCYRITPQGSHVLDLVVERSARYWTGRAVPEYTLL"
     gene            16333..17049
                     /gene="bluB"
                     /locus_tag="VFJ72_07990"
     CDS             16333..17049
                     /gene="bluB"
                     /locus_tag="VFJ72_07990"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: protein motif:HMM:TIGR02476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HET7479437.1"
                     /translation="MPRRSSEGVAASPAAGELSLDEKRGLYAAIFGRRDIRAFRPDPV
                     PDEVLARILDAAHHAPSVGFMQPWDFILIRDAETRQRIKELSDRERQAAACFFDEPRR
                     DKYLSMKLEGILDAPLNLCVTCDPTRGGPQVLGRNSIPETDVYSTCLAVQNLWLAARA
                     EGVGVGWVSILRNAELREILGMPHHIIPVAYLCMGYPRDGFPEEPLLQTTGWRDRIPL
                     DSLVRHERWDDAREDESTLR"
     gene            complement(17041..17856)
                     /locus_tag="VFJ72_07995"
     CDS             complement(17041..17856)
                     /locus_tag="VFJ72_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET7479438.1"
                     /translation="MLELQRNVIFGQFVDTGSFVHRMDARIKLAITLVFIVASFLIHS
                     FLGFAVALPLLVVIQIVSRIPIGYLLRGSQLFLGFLVFILAFQVLFFTGEVPEGGYLW
                     RWGFLSISTTGLYTAGILGLRVIFLYYVTTMLMLVTSLVDLTNGLELIFGPLQRIGVP
                     VNELVLVFVIAIKFVPIFIEEVERLARAQTARGVPFDEGGPITRARRIGRLLVPIFIS
                     GFARADLLSTAMHTRSYRGGRYRTKFRRMKARASDWAVLALVTVWMAAAWLLP"
     gene            complement(17908..18837)
                     /locus_tag="VFJ72_08000"
     CDS             complement(17908..18837)
                     /locus_tag="VFJ72_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479439.1"
                     /translation="MSRLFDVYMAVDWSSTNSRSKARPSKDAIWVGERLAFGFRGYED
                     PGEAYFRTRMDCWKQLRGRLLRHASRGLRVFIGFDFAYGYPAGFSAALGLDGHLPLWR
                     SVWEEISRNITDADNNINNRFEAAAVLNARCGGDRPGPFWGCPEKRQQPALSMRSPGF
                     PYSVGRGLDLERLRITDRAQRGVQEVWKLYGAGSVGGQALVGIPVVRRLRDDVRFADF
                     SSVWPFEAGFVSEPTPDRGPFILHAEIFPGNVPDPLDTDVIRDQAQIRAVVRWLSRLD
                     EQGELARQFATPDGLTSESIEVCVKEEGWILGS"
     gene            complement(18879..>19071)
                     /locus_tag="VFJ72_08005"
     CDS             complement(18879..>19071)
                     /locus_tag="VFJ72_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HET7479440.1"
                     /translation="GTVVMDGPVREVLKKADRLAELDITLPEPVIGAREMRKLFPDFP
                     TELLTEEELEAELMKRLGT"
BASE COUNT         3517 a         6449 c         5879 g         3226 t
ORIGIN      
        1 ggcgatccag gggtatccgg tgcctggggc gtgtccggga ccgttgtaga gggttccctc
       61 gtcaacgccc tctccgatag gctcgcctga cttacccatc gcgggaaccg taagcgtcat
      121 cgccgtgctg ggcggcgcgg gcgctccaga cggcgaagcc gtcccggaag gcgagctctc
      181 cgattgcgcc accgggggct gccgtgagga tggttgctcc tctatcgtct gccctgccga
      241 agcctgtccg gccgacgttc gatcctcccc cgcagcgcgc ttcgctgtct cccgatgcgc
      301 ggcggctttc tcctctgttg tctgcttcgc cagcttctct tccgctgcgc gcctggcggt
      361 ttctccggcg ctccgcgccc gggcctctat cagggcgtcg ttgaccagcg gctctggagc
      421 cggaagcact acgggagtca cggcactcgc gaccgtaccg gcgccggtgc tcaagactct
      481 ttctccgagc acgagcacgc cgagtaccag accagcggct atcaacagcg agtagaaggc
      541 cctccggcgc gatctccggc gctcctcaag gcgctccttg cggctgacct ccatctcggc
      601 cgcgtccggt gagtatttca ccctgcccat ctctcgtctc ctggtcttca tgctctcgtc
      661 ctctcggctc tctccagccc gttgccggta ccatcaatat tgatcctcgc ggcgagtcgt
      721 gtcatggtcc aaatgagtga tttttgcgtc ggagatggag cccctggcac acgatatggt
      781 ccgccgcgta gcggtccggc cataagcgtt ccgggccgca gaagaaacgg gaagaccgca
      841 gctagttgga cctgtctata agtcacatgc gagactctgg agcgaggtac aatagctcgc
      901 gcatggagag tattcggtaa agttcggtgg tggcgttcgg gggtggaagg aagccgctgc
      961 tggtaccgga ccccggccag aagatagctc tgatctccga ggacggctcc accagggagg
     1021 gcttccgggc cgtctcggga ccgctgacgt cggatacggg agagacgata gtccaggtcg
     1081 tcacggagcg ggagtaccag gatgccaggc tggacggccg tcccccggtc ttcctgagtt
     1141 ggcccgcaaa ccgagtaacg ttcccggttc cgaggtactg gtggctgaag cgccggttct
     1201 ccagataaac acctctgaac ctcgcctcaa acccgccggc aaaagagaca cgtgatgtgg
     1261 gcctgcctgg actcgaacca gggacctctt ccttatcaga gaagcgctct aaccggctga
     1321 gctacaggcc cacggatgct gggaatattc cagccgggat aatttacaac accgggcctt
     1381 ccctgacaac ctgattgaag accggagagg cgggaagtaa aatcgagtgc gtgtcaaacg
     1441 gactctcaat ggacaatcac agcctcacgg tgtgagagga gcaaaaaacc agtgagcgat
     1501 aacggcctca cccggatgtc catctacggt atcaacatgg acctcttcac ctccagcccc
     1561 atcgtcatac ttaaagtcga ggaggagaac cgctatttgc cgatctggat cggccagtcc
     1621 gaggcgcgtt cgatcctgat gaagctgcag aaccaggagt tctcccgtcc tctcacacac
     1681 gacctcgccg tgaacctggt ctccgagctt ggcggctcga tggaacgggt catcgttacc
     1741 cggctgcagg actctacctt cttcgccacg ataagcgtgg atatcggtgg acgcacggtc
     1801 gagatagact cccggccctc tgacgccatc gcgctggcgg tgcgctccgg agcggagatc
     1861 ttcgccgcca acgaggtcat agaggaagcc ggcgtcgtct tcgaggaggc gatcgaggac
     1921 gcccccgagg aagaggtcgt cgacaagttc aaggactgga tgaaccgggt ctccccggaa
     1981 gacttcaagt agtgatccgg taagtccagt aagagcggct aatccttgga gcggccgaag
     2041 agcccttggc gttcctcctc ggccttcttg gcgcgttcgg cggcccggct cacggcgatg
     2101 gtggcgaaga tgaccacgag cgtgatcagg accgcgtaga taaactggcc gatcaaggcc
     2161 gctcccgcct gaccgaagat cttgttgaag agattctgga tggccgtgtt ccaggcgagg
     2221 gccgccacca gacccagggc cgtcgagatc aacgccgaaa acttgtccag cacttcaccg
     2281 gtcaactgtg cctcctttta acttgtcgtc ttcggtcccg ctgctgttcg actcttctac
     2341 acggtactcg gggtaggccg agaggaactc ctcgaccgat cccgtacccc agcccacgat
     2401 aacctccatc gtgctctggt tgaagcgggt ccggatctgg ccttccctcc tgacgctgcg
     2461 gcggacatag gcccagggcg tctcgccgaa acggcgctcc agccgctcgg tgtagacgaa
     2521 ccggcccgtc gcccggtccc gcaccatcag taggtccact ctcagccccc cggagctttc
     2581 aaccccgcca accgttccag caaccccgct tcttcccacg gccaccgtcc ttcatcctct
     2641 tcaaccccga acggcggcac gctgccccac atcttacctg ttccggcagc tacactacta
     2701 cccgccacga ggacttcccc ctcgccgagc gtcgccaggc cccgccgatg cacgaacgat
     2761 ccctctcgct cctcgatccc tccgacgccc caggatgccc gtatgcgaag atcactcccc
     2821 atatcccgga gcgcccgccg cagggcgtac agcaacagcg cggcccgccc gtccgcgaag
     2881 ttctccgccg aacgcagagc cgccagcagg tccaccgcct cttcggagcc aacgggcgcc
     2941 acgggcaggt cctggtccag ttcgaactca ccactccaga cccttctcag gattcgctcc
     3001 cggtgagcgg acaccgccag ccggccgagg tctccagacc cggcgcggac ccggatggcg
     3061 agggcccccg gtccgatccc cgcctcctcc actctctccg cgtcgaacgg ctcgcccacc
     3121 acgtaaacgt ctatctcgct cccgagacgt tcctcccaac tccggctacc ggtggtgatc
     3181 gcccggcggt ccagggagtc gggctcgccg ctacgggaac cgaagaacgg caggtcctcc
     3241 tcctcgatca gttcgtcctc cgccgcagcc cgcgccccga ccccgcagac ccggcgcacc
     3301 ccggcggctc cgagcgccgg aatgccgact tcggcgagtt tgcggaggat catgggcgcc
     3361 acgtcctcgg gccgcacccc gagaccccct accggctcat cgctcgtgag cagcatccct
     3421 ctcacgggga ccgacgcgag gtccggggct acgctctccg ttgaggcggc tacccacccg
     3481 aacccggact ccgaggccgc cgcgtagacg gggctctcca caccgggcag gcgggcggcg
     3541 aagagtgcgc gctcgaaggc cgagctgtcg gcggcgtaga ggacgacctc ggcttcatcg
     3601 ccctcgccgc gccactcgta gtccgccaca tgctcggcca cggaagaccc gtttactcta
     3661 tgacgatcag aggatcgccg gagcggacgg tctgcccggc ctcgacgcgc acctcggaga
     3721 cggtgccgga cttgggagcc cggatctcgc tctccatctt catcgcttcc agcacgcata
     3781 ccggctcgtc cgccgctacc tcctgcccct cctcgacgag caccttgaca atggtcccct
     3841 gcatcggggc cgcgacagcc ccctggctca ccgacgcccg ccgggtctct cctgtcttgc
     3901 gccggggcgg cccgcccccg ccgttcgcca cctcatcccc aaataccttg acccggaaga
     3961 gcctgccgtt gacctcgacc tcgacctccc gcgcgcgctt ctcggaggct tcgcctctcg
     4021 cctcgatctt cccgagcgat gccggctcta tctttagccc gtccatcctc tccgctataa
     4081 agcccgtcgt gtagtcgccg gagatgaaga cctcgtcgtc caggatcgcc cggaagaacg
     4141 gcagcgtcgt cgctatcccc tcgacccgga actcagagag cgcccggcgg agcttcgtca
     4201 gggcatcctc ccggtcggtg ccgcgcacga tgagcttggc gaacagcggg tcgtaggact
     4261 cgggcaccac gcccggtccc gagagcgagg agtccacccg cacccccgga ccgcccggct
     4321 cttcgtaggc ggtgacgccg ccggggctcg gcgcgaagtc gttggcggcg tcctcggcgt
     4381 tgaggcgcgc ctcgatggcg tgaccgcgcc aactcacatc ctcctgagcg aagggcagcg
     4441 gctcaccggc ggcgatgcgg atgccggtct tcacgatgtc tatacccgtt acctcctcgg
     4501 tgacgggatg ctcgacctgt accctggtgt tcatctccag gaagtagaac tcgtgatcct
     4561 ggaccaggaa ctcgacggtg ccgacgttgt cgtagccgac ggcctccgcg gcctcgaccg
     4621 ccgcgtgtcc catctcctgg cgcatggcgg gtgtcagggc cgggctcggg cactcttcga
     4681 ggagcttctg gtggcggcgc tggatggagc agtcccgctc gccgagatga acggcgttgc
     4741 catgcttgtc cctgacgacc tgtatctcga cgtggcgcgg tcccggcagg tacttctcca
     4801 cgtagaccga tccgtctccg aagtacgcct ctccctcgcg gctggcccgc tcgaacgccc
     4861 cctccgccgc cgcatcgtcc tgggcgaccg cgaagccctt gccgccgcca cccgccgaag
     4921 ccttgaccgc gacgggatag ccgtgctcct ctccgaagac gaggacctcc ttggcggagc
     4981 ttatggagtc tttcgtgccc ggcaccatcg ggacgccggc cttcatcatg atgcgcctcg
     5041 actcgacctt gctgcccatg gcctctatgg cctcggggtg cggcccgatc cagacgagcc
     5101 ccgcctccgt gaccgcccgc gcgaaaggcg cgctctccgc gagaaagccg tatcccggat
     5161 gtaccgcctc cgctccgctg cgcttcgccg tctcgacaag ctttgcgatg ttcaggtagc
     5221 tctccggcgc cggcgccggg ccgatgtggt acgcctcgtc ggcgagggcg gcgtggaggg
     5281 aatcgcgatc cacatccgag tagacggcga cgctttctat gcccatctcc cgcagggcgc
     5341 ggatgatgcg gacggagatc tctccgcggt tcgcgatcag gaccttagag aacacctaga
     5401 ctatcccctc ccaactagag taagaccaca aagaaccttc gagcgtcatc cccggcggca
     5461 cctgatgtcc gagcagaccg gccagcttcc accggctgcg cttgagcgaa gatcccttct
     5521 ccggctccgc ctcgcgcaac gcctccagcg cggccatgat ggcggcggcc tcttcgggcg
     5581 tcggaacggg ctccgtatat attcgcctgg tctccgtcat ccctccacgt accggatcaa
     5641 cggcgaaatg agatacacgg tcaacgctag tccgaccaat acccccacta cgaacccgcc
     5701 gagttgaagt ccaagcgtgc taagcatcag gctcattcta tcccgcgaag cgttctccga
     5761 tccgccttcg atcatcaccg tcagagacga ccagaagccg agaggacaac taaacaggag
     5821 cccgaaggtg aacgtccctg cattaccgga actccagtcg ggagcaccga agagaagctc
     5881 ggccgagaag agggttgcga ccagcaccac gtgcaacagc caggcacgga gaagtatcat
     5941 cctccgtccg ccccaagggc gcaggcgaaa aaagacgcgc gcgattagat agccaacagc
     6001 cccgccgatc accaagctgg aaaacagggg cagcatcggg gtgcctgcct tcgtaccggc
     6061 aaagacgagc atgaccagca agacgagcat gaacccgaag aaaagaaccc gcgccttcat
     6121 cccgcgaagt accggatcag ggggccgagg atgtactgaa tcagagcgta caggccgaaa
     6181 gccagtgcca gaatagtaaa caacgcaccg gctactatca gtatcccctg caaaatcgcc
     6241 cggcggctct ggtagacctg ttccggtccg ggttgcggtc gaggcacaag tcctgaccga
     6301 agcccttgcg ggactcccag agcgagtcca tacacgagcg ccccggacat tcctcccccg
     6361 tcggagaaaa acgacggcga acctacgaag aacgcgccta tcaacaagag catgagcccc
     6421 agagcgaaac ccgaaacgaa ggctacaacg tctgcccaag aatcaaagcg tcggacaaat
     6481 cggcccctaa acggcataga caccgagtaa gccgataagt gcccgattag tactccgagg
     6541 agcacaacca gcacccaaaa accggtgtcg gaacccatca ggcccagcag agccagagcg
     6601 attgccagag cagaaattag tgcaacgccc gcgcgctcag gccacgtctt gaaccacctg
     6661 tcgaagatca cgttcatgct cgcctcttgg atttcctcaa agcgggatgt ttccgtgctt
     6721 gcgcggcggg cgctcgggac gcttggtcct tatcatctgt agcgccttta tgagatacgg
     6781 gcgggtctcg cgcgggtcta tgacgtcgtc cacgaatccc atctccgccg cgaccatcgg
     6841 gttgttgaac tgctcctcgt agccttcgat gagccgctcg cgctccgcct ccgggtcttc
     6901 ggcctcggct atctggcggc ggaagatgat gcccacggca gccgatgctc ccatcaccgc
     6961 aatctctccg gtcggccacg acacgttgac gtccgcccct atgtgcttgg agttcatgac
     7021 gtcgtaggcc ccgccatagg ctttgcgcgt gatcacggtc atcttcggca ccgtggcctc
     7081 ggagaaggcg tacaggagct tcgccccgtg acggatgatg ccgccccatt cctgatcggt
     7141 gcccggcatg aaccccggca catcaacgaa cgtcaccagc ggtatgttga aggcgtcgca
     7201 gaacctgacg aaccgagccc ccttgacgct cgcgtctatg tcgagcgtcc ccgcaaggac
     7261 cgccggctgg ttccccacga ccccgaccgc gtgcccatcg agccgcgcga agccgactac
     7321 gaggttctga gcccatccct cctgcacctc gaagaactcc ccatcgtcca tgatactcac
     7381 caccgcctcc cggatgtcgt aggggcggtt ggccgagtcc gggacgagcg aggcgaggtc
     7441 ctcgtccatg cgctccggat cgtcggccgg cttgtagtac ggcgcgctct ccaggttgtt
     7501 ctcgggcagg aacgagagca ggtagcgcac gtcgtccaga cagctctctt cgtcgggggc
     7561 ggagaagtgg gcgacgccgg acttggtgtt gtgggtagca gcgccgccga ggtcttcctg
     7621 ggtcacgtcc tcgccggtca cgctctttat cacgtccggc ccggtgatga acatgtggct
     7681 ggtctcctcg accatgaaga tgaagtccgt tatcgccgga gagtagacgg ctccacccgc
     7741 gcacggcccc atgaccacgc ttatctgcgg gattacgccc gaggcgagca cgttacggtg
     7801 gaagatgtcc gcgtatcccg cgagagacac gacgccttcc tgtatccttg ccccgccgga
     7861 gtcgttaatc ccgactatgg gacagccggt attgagcgcc aggtccatca ccttgcagat
     7921 cttctgcgcg tacacctcgc ccagcgagcc gccgaacacc gtgaagtcct gcgagaagac
     7981 gcacaccttg cgccccgaga cctcgccgta gcccgtgatt acgccatccc cgagtggccg
     8041 gttctcctcc aagccgaagt tgtacgagcg gtgcacccgg taacggtcaa gctccacgaa
     8101 ggtgccttca tcgagcagta gttcgatccg ctcgcgggcg gttagcttgc ccttctcttt
     8161 ctggcgctcg accgcctttt cgggtgctgg atgcgccgct gcctcgcgga gtttctccag
     8221 accttctatc tttccggcgg tggtattctc gccgagcctc tcaccctgca tggttcggca
     8281 ttctagcacg cgcgaaagcc acgatttcat ggcgcgagtg ttgtgggcgt tcggcgggcg
     8341 aagaccttac cggatacgga tacgcttctc ttccactatc cagagacggc cttcaagagg
     8401 ttcgtgttcg accgccttta gcgttcgccg gaacacgtcg agaacgtgga ccttatcttg
     8461 ccgggcaagc cgcagcacga tcaggccggg atgctcgtcc ggcgggtagg tttgtatgtc
     8521 ggcgaaatcg agatccaggg tcacgaagac ccggccttcg gtcttacaga cctcggacag
     8581 atgccggtcg ctggcgccga cgagccgttg atcgtccacc gtagcggcct cgtgtccggc
     8641 ggcccgcaac tcctcggcca gctccaccgg gaggttctca tcgatcttga acctcacgcg
     8701 gcgccttacg ccggcagggg tacgacgcgc tcgcgggcca gatccgcggc gtaggcaatg
     8761 gccgcctgta tgtcctcgcg accgacagaa gggtagctct ccgcaatctc ctccggcgtc
     8821 tgaccggcgg cgaggttgtc caacaccacc gaaaccatca ccctggttcc cttgatgcag
     8881 ggcttgccgt gacagacatc gggatcaacc gtgatgtgtt ctctccaatc catcacgagt
     8941 agtttaaacc ctgaccaccg aggatcgctc tctcgttcat cgcctttaca agaatagaat
     9001 ccgaattgca tttcaacggg cacattaccg gcttttcgac ccaccctctc accctgcatg
     9061 gttcggcatt ctggcacgcg cgaaagccgc attttcgccg cggatacggg ctcgggtagt
     9121 agaataaaga agttccggga tcgccaccgg cgtgggaaag gagaatagcc tatgcaagtc
     9181 ttcgagaagc tttcggagta ccgctacgag cagctcgtct tctgccacga caagtccaca
     9241 ggactgcgcg cgatcatcgc gatccacgac actacgctag gtcccgccct gggcggctgc
     9301 cggatgtacc cctacgccac cgaggacgaa gcggtggttg acgcgctgcg cctggcgcgc
     9361 ggcatgacat acaaggccgc cgcgagcggg ctcaacctcg ggggaggcaa gtcggtcgtg
     9421 atcggggacc cgcacacgga caagtccgag gcgctgttcc ggtcgctggg acgctacgtc
     9481 gagacgcttg gcggacgcta catcgtggcc gaagacgtcg ggatctcgac ctccgacatc
     9541 tcgtatatcc acgtcgagac ctcacacgta gtcggcatgg acgtaaccta cggcggctcc
     9601 ggcgacccct cgcccttcac cgcgctcggg gtgatgcagg ggatgcgggc ctgcgccgag
     9661 gaggtcttcg gtacggactc tctggagagc aggacggtcg cggtccaggg gctcggtcac
     9721 gtcggctacc acctgtgccg cctgctgcac gaggaaggcg cgcggctcat cgtgacggac
     9781 atcgagcggg aagcggtcga gagggtcgcg cgggagttcg gggcgaaggt ggtggagccg
     9841 gatgagatcc ggacgatgcc ctgcgacgtg ttcgcgccgt gcgccatggg cgccgtgttg
     9901 aacgacgcca ccatcccgga ccttcgctgc gagatagtgg ccgggagcgc caacaacgtc
     9961 ctgctggagg cccggcacgg agaggccctg gcggatcgcg gcatcctcta cgcccccgat
    10021 tacgtcataa acgccggagg gctgatcaac gtcgccgacg agctggaggg ctacaacgag
    10081 cggcgagcca cccggcgcgt catgcggata aaggaccgga tacgccagat catcgccatc
    10141 tcaaaacgcg acggcgtccc gacgaacgtc gccgccgaca ccctggccct ggagcgcata
    10201 gccgccataa gctcgatgga acgcctgcac accggccacc cgtacggcca actccagcgc
    10261 cggcgcgaga cgatctagga ccgcttcttc tctactcctc ctcgcgccta tgtcccgatg
    10321 tcatccggcc tagccctcga ccgccgctcc cgctatccgc tccagtagag cgtcgacgct
    10381 ggtaagctcg gctccgtaca ctttctccag atactccaga ccgccggtct gatcctcctc
    10441 cgtaaacgcc tctactgcgt cagccgggac caccaccttg tatccccggt agaaggcgtc
    10501 tgccgccgtg tgcctgacac agatgttggt gtgcagcccc gtgagcacca cggtgtccac
    10561 cccgagctcg ccgagcagca tttcgaggtc cgtgccgtag aacccgctgt agcggcgctt
    10621 gggcactatg tagtcctcat cctccggcgc gagcgcgccg ataacctccg ctccgggagt
    10681 gccggccacg gcatgcggcc cccacacctc aaactcgtgg tctatctccg gaagatgcgc
    10741 gtcattggag aagatgaccg gcgtaccggc ggcccgcgct ctacccagca gcctaccgag
    10801 cggctccaat atcccgtccg ccctctcgca cctgagccct ccgtaaacga agtcctccaa
    10861 catgtcgata actaagatcg cggctctcat ccgtacctcc ttgtaacgcc tacccgactt
    10921 gttgctacat gaacttgata caagcaacgg ttcttgtaac aaaacactac atgaagtatc
    10981 acactgtcgg caaacatctg caagcgttgc gtgtaattct cgggaggtta ctgggcgcta
    11041 ttgcggtcgg gtcgtattcg ggctggcggc tggcggggac attcggtagg atgcgtgact
    11101 gtagacaccg gggagctcca ggccaggggc tgagagggtg caggatccgc gccgacccga
    11161 agaacctgat ccgggtaatg ccggcggagg aagggtatgg gctcatatat cgaggggacg
    11221 tccggggctt cggctacggg cgtttttgcg tctccgccgc gcccgggtcc agacgaagga
    11281 ggcaggagat gctggagcaa cgcggaacgc ggcggccggg cgaggctggg gagtcggtaa
    11341 cccagctccg ctacgcgcgg gagggcgtca tcacggagga gatgcgcttc gtggcggagc
    11401 gggagcggat ggaggccgag gtcatccgcg aggaggtagc gcgggggcgc atgatcatac
    11461 ccgccaacgt caaccacttc tcgctggagc caatgtgcat cgggaaggcg gcccgcgtca
    11521 agatcaatgc caacatcggc aactcccaga ccacctcgga gatagacgcc gagatagaga
    11581 agctccgcac ctccgtgaag tacggcgccg acacggtgat ggacctctcc tccggcggtg
    11641 acataccgag gatacgccgg gccatcatag aagccagccc cgtcccgatc gggacagtcc
    11701 cgatctacga ggtcataagc cgcgtagata gcccggaaga gatgaccggc gacctcttcc
    11761 ttgaggtcat ccaggagcag gccgagcagg gcgtagacta catgaccatc cacgccggag
    11821 tcctcttgca acacctgcac ctggtgcaga gccgcatcac cggcatcgtc tcgcgcggcg
    11881 gtgggttgct ggcgaagtgg atggtccatc acatgaagca gaacccgctc tatgagcggt
    11941 tcgacgagat cctggacata tgccgccgct acgacgtctc catctctctg ggcgacgggc
    12001 tccgccccgg cagcgtcgcc gacgcctccg acgacgccca gttcgcggag cttcgcactc
    12061 tcggcaagct caccgagcgc gcctgggcaa aggacgtgca ggtcatgatc gagggtcccg
    12121 gacacatccc gctcgaccag ctagagatga acgtgaagat gcagcaggag atctgccacg
    12181 aggccccctt ctacaccctg gggccgctcg tgacggacat cgcgcccggc tacgaccaca
    12241 ttacgagcgc tatcggagca gcagtcatcg gctggcacgg gacatccatg ctctgctacg
    12301 tgaccccgaa ggagcacctg ggtctcccga acgccgagga cgtcaagcag ggcatgatcg
    12361 cctaccgcat cgccgcccac gccgcggaca tcgcccgcgg acgtcccggc gcccgcgagt
    12421 gggacgatga gctatccagg gcacgctacg ccttcgactg gaggcggcag ttcgatctcg
    12481 ccctcgaccc ggataccgcc cggaacatgc acgacgaaac cctcccggac gattacttca
    12541 agacggcgga gttctgctcc atgtgcggcc cgaagttctg ccccatgcac aacttccgcg
    12601 acgtggactg gggcgccctg aagaaggcca ccgaccgtct gccggctggg ggctgagtgt
    12661 ccggtaagta gcgagaggga gacgccggag cgtctccctc cccgatctgc cacccggagg
    12721 ctgaaagcag tggcagatcg ggtgcgcggt tctcgtccgc acatgctaca ctgggatcga
    12781 agtacgctag gggtgcttta aaaagctgag agcagaccct tggacctgat ccggatagta
    12841 ccggcgtagg gaagcgtgga tacgggcaac gagactagcg ttgccgccgc gccgcccgga
    12901 gcccggcggc ttttttgtgg agccgccgtg ggaccgaaag gctccgtttt ggcgcgtgac
    12961 ctcgatatac acctcataac cgaccgcaaa cgggctcgcg gcgacctgcc ggaagtggtg
    13021 cgcgccgccc tgctgggtgg ggtggacttc gtgcaggtcc gggagaagac tggcccggcg
    13081 cgcaacctct acgagaccac tctacagata ctccctcacg cccggaaagc cggggccgga
    13141 gtgctggtca acgaccgggt ggacgtggct ctcgccaccg gcgcggacgg cgtgcacctc
    13201 gccggcaaga gcttgccgcc ccgtatagcc cggaagctaa tcggcgagct tctactgggc
    13261 gtctccgtac acggtttggc cgaggcccgg gaggccgtga gcggcggcgc ggactacgtg
    13321 accttcggcc acgtctatcc cacctcctcc aagcccggtc tgccgccgcg gggcctgatc
    13381 gaactggccg agatcgtcga gtcggtgaac atccccgtgc tggccgtggg cggcatagaa
    13441 ccctcgaacg ccctggaggt gctgaagacc ggggtcagcg ggatctcggt tatctccacc
    13501 atcctggcgg ctcccgaccc ggagaaggcc acgcgagagt tgcgcgggat actggatgtc
    13561 tcagggcttc gcccccggcg ccgcttcccc aaacagagaa tgaaaggagc gagatgaacg
    13621 taaccctcaa cagagagacg gtcgagacag agaacccgga acccaccata agcatcctgc
    13681 tggccgaacg cggcatatct gaggaaggca tcgtcgtggc ggtaaatggc gacgttataa
    13741 ggcgcggcga atgggaggaa cacgtggtaa gaagcggcga cgaagtggag atagtgcacg
    13801 ccgttgccgg ggggagcggc gaagacgaac tggagatagc gggcgaggtc ttctcctcgc
    13861 ggctcttcct cggcacgggc aagtactccg acgcggagac catgaacgcg gcgctcgcgg
    13921 cgtcggagac cgggatggtg acggtggcga tccggtacat ggacctctcg ggagacgggg
    13981 cgtcctcgat cctggaccag ctcgacctct cgcgctaccg gctgctgccg aacaccgccg
    14041 gggcgacgac cgccgagcag gcgatccgga tggcccggct cgcgcgcgag gccacgggca
    14101 cgaactggct caaactggag gtcatcggcg accaggacac tttatggccc gacgtggccg
    14161 cgacggtgga ggcgacgaaa gtcctggtcg aagaaggctt cgtcgtcctg ccgtacacca
    14221 gccccgatat agtcgccgcc gtccggctgg aggaggccgg agccgcgacc gtgatgccgc
    14281 tcggctctcc cataggctcc ggtcagggaa tggtcgactg ggcgggcatc cggcgcgtga
    14341 tcgagcggat ctccgtcccc gtggtggtcg acgcgggtat cggcgtcccc tccgacgccg
    14401 cgacggcgat ggagctcggc gcggatgccg tgctcgtcaa caccgccata gcccgcgcgg
    14461 acgacccggt ccgcatggcg gaggcgatga agctcggcac cagagccggc aggctagcgt
    14521 acctcgcggg ccggatgccc atcagcggaa cggcccaccc gtcgagcccg acccgcggcg
    14581 tcccggcggt gagcgcctcc tgacgtgaaa gccagcgtgg acgtggccgt gatcggcggc
    14641 ggggtcatcg ggtgctccgt cgcctaccac gccgcccggc gcggggcgag cgtcgcgctc
    14701 ttcgaggccg ggcagatagg ctcgggcgcc tccggggccg ccgcggggat gctcgccgcc
    14761 caggcggagg cgcgagagcc cggcccgttc ctggacctcc tgctggagag ccgcgagatg
    14821 caccgggcgt tcggcgagga actgtacgaa gagaccagcc tcgatcccga gtacgtgtgg
    14881 gcggggaccc tgcgcgccgc attggacgaa ggcccggtgg aggaactccg cgcccggcat
    14941 ctctggcagc gtcgacacgg cctctccgcc cggtggatga gcggggatga agcccgcgag
    15001 ctggaaccgg ctctctcctc ccggacggtc gcggcgctct acctgccgga ggacggtcag
    15061 gtgaactcgc cccggctggt gcggtcgctc tcgctcgccg ccgcgcgcag cggcgccagg
    15121 gtagaggagt tcacgaaagt ctgcggactc gttgtcgagg ggtccactat aacgggcgtt
    15181 cgcacctccg cgggagagac ggccgcgggt gccgtggtgc tggcgggggg cgcttcgagc
    15241 gcctcgctcg tcagtgatct gggtgtggag gtcccggtgc atccggtgaa gggcgagatc
    15301 ctcgccgccg tcccgccgtc tctgcccata aaggccaacg tgtgggacga gcggtgctac
    15361 atggtcccca agcgggacgg ccgggtcgtg atcggggcca ccgaagagcc cggcgtccgg
    15421 gacccccggc ccaccctggg aggcgtcgcg gccctctccg gcgcggcagt gaggctggtg
    15481 cccgagatct cggccgcgac gttcgccagc gcgtggggcg gcttgaggcc cggcactccc
    15541 gacaaccttc ccgtactcgg tccggtagac ggcgtcgaag ggctgatgct ggctaccggc
    15601 cactaccgca acggcgtcct gctagccccg cttaccgggg aggtgatctc cgccctcgcc
    15661 ctcggagaga ccccgactac ggacatctcg cccttctcgg cgcacaggtt tacgagggcg
    15721 gctcacactt gatcgagctg ccggcggatc tgctagtaac gatggtatcc cacgcccgcc
    15781 gggaggcgcc gcgcgaggtc tgcggatggc tggccgggag cgacggcgga ataatacgcg
    15841 ttctccccgt cccaaacgcc gccgaagacc cactgacccg gttcctcatg gagcccgtcg
    15901 cgcaggtccg cgccatgcga agagtagcga agatgggcct ggacctcatc ggcacctacc
    15961 actcccaccc cagaaccccc gcgacacctt cggcgcggga ccgtcaactc gcggcgtact
    16021 cggagatggc gcacctcatc ctgtccctgt ccaacccgga accggagatc ggttgttacc
    16081 gcatcacccc gcaagggtcc cacgtcctgg atctggtagt cgaaagatcc gcgcggtact
    16141 ggacgggacg ggccgttcca gaatacactc ttctctgaaa actgatgcgg cgggctcatc
    16201 cgccgccaca gggaggaaca cagatgccag aagctggccc cagatcggtc aagattactc
    16261 taccgcgtag tcctcgtcgg cgccttcctc aatccagaac acgaatgaac acctctctga
    16321 gaggagccgc cggtgcctag aagaagctct gagggcgtcg ccgcctcacc cgctgccgga
    16381 gagctttcgt tagacgagaa gcgcggtctc tacgcggcca tcttcggccg ccgcgacatc
    16441 cgggcgttcc gccccgatcc ggtcccagat gaagtcctcg cccgcatcct cgacgccgcc
    16501 caccacgctc cctccgtggg gttcatgcag ccgtgggact tcatcctcat ccgcgacgcg
    16561 gagacgcgcc agcgtatcaa ggaactctcc gaccgggagc gccaggcggc ggcgtgcttc
    16621 ttcgacgagc cgcgccgcga caagtacctc tccatgaagc tggaaggcat cctggacgcg
    16681 cccctgaacc tgtgcgtgac ctgcgacccg acgcgcggcg gcccgcaggt cctgggccgc
    16741 aacagcatcc cggagaccga cgtgtacagc acctgcctcg ccgtccagaa cctctggctc
    16801 gcggcccgcg ccgagggcgt cggcgtcggg tgggtgagca tcctgcgtaa cgcggaactg
    16861 cgcgagatcc tcgggatgcc gcaccacatc atcccggtcg cgtacctctg catgggctat
    16921 ccgagagacg gtttcccgga ggaaccgcta ctccagacca ccggctggcg ggaccgcatc
    16981 cctctggatt ctctggtgcg gcacgaacgc tgggacgatg cgagagaaga cgagagcacg
    17041 ctacggtagc agccaggccg ccgccatcca gacggtaacc agcgcgagta ccgcccagtc
    17101 gctcgccctg gccttcattc gccggaactt ggtgcggtat cggccgccgc ggtagctgcg
    17161 ggtgtgcatg gcggtagaga ggaggtcggc gcgggcgaag ccgctgataa agatcgggac
    17221 gagcaggcgt ccgatgcgcc gggcgcgcgt gatggggcct ccctcgtcaa acggtacgcc
    17281 gcgcgcggtc tgggcgcgag cgagacgctc tacctcctcg ataaagatgg ggacgaactt
    17341 gatcgcgatg acgaagacca gcaccagctc gttgaccggg accccgatcc gttgtagcgg
    17401 cccgaagatc agctccagac cgttcgtcag gtccacgagc gaggtgacga gcatcagcat
    17461 agtcgtaacg tagtacagga agatgacgcg caggcccagg atgcccgccg tgtagagtcc
    17521 ggtggtcgag atggagagga acccccaacg ccacaagtag ccgccctccg ggacctcgcc
    17581 ggtaaagaag agaacctgga aggcgaggat gaagacgagg aagcccagaa agagctgcga
    17641 gccgcgcagc aggtagccga tcgggatgcg ggagacgatc tggatcacga ccaggagcgg
    17701 cagcgccacc gcgaaaccga ggaagctgtg gataaggaac gaggcgacga tgaagacgag
    17761 cgtgatcgcc agctttatcc gggcgtccat ccggtggacg aacgagcccg tatccacgaa
    17821 ctgaccgaag ataacgttcc gctgaagctc aagcattagt tatgcccatg cacactgagt
    17881 ctggccttcg ccatcgcgtg catcctgtta gctgcccagt atccatccct cttcctttac
    17941 acaaacctca atggattcgg aggtcaagcc atccggcgtg gcgaactgtc gagcgagttc
    18001 cccttgctca tcaaggcggg aaagccagcg cactacggct cggatctgag cctggtcacg
    18061 gataacgtcg gtatcgagag gatcgggtac gttgccgggg aagatctcgg catgcaagat
    18121 aaacggcccc ctatccggtg ttggctcact gacaaaccca gcctcgaagg gccaaacgct
    18181 gctgaaatcc gcaaacctta catcgtcgcg cagccggcgg accacgggga taccgacaag
    18241 cgcctgtccg ccaacgcttc cagccccgta caacttccaa acctcttgca cgccgcgctg
    18301 tgctctatcg gtaatacgca atcgttccag gtccaggcca cgcccaacac tgtaaggaaa
    18361 tcccggactc ctcatactca aggcgggttg ctgacgtttt tcgggacaac cccagaacgg
    18421 gccgggccga tcacctccac agcgtgcgtt caacaccgcg gccgcctcga agcggttgtt
    18481 gatgttgttg tcggcgtcag ttatgtttcg agaaatttct tcccacacag agcgccacag
    18541 cggcaagtgt ccgtcgagtc ctagggccgc tgagaaaccg gccggataac cataagcaaa
    18601 gtcgaacccg ataaacactc gtagaccgcg gcttgcgtgc cgcaaaagcc tccctcgaag
    18661 ctgcttccaa cagtccatcc gagtgcggaa gtaagcctcg ccgggatctt cgtatccgcg
    18721 gaaaccaaac gccagcctct caccaaccca gatcgcgtct ttcgagggac gcgccttcga
    18781 acgcgagttg gtggagctcc agtctaccgc catgtagacg tcgaatagcc gtgacatcta
    18841 accgcctcct ccaactttcg gtttgcgaag ctctcggttc atgttcccaa ccgtttcatc
    18901 aactcggcct ctagctcctc ctccgtcagc aactcggtcg ggaagtccgg gaagagcttg
    18961 cgcatctcgc gcgccccgat gaccggctcg ggcagggtga tgtcaagctc cgcgagccgg
    19021 tcggctttct tgaggacctc gcgcactggt ccgtccataa ctaccgtgcc c
//
LOCUS       DASSMB010000131        23327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1802357, whole genome shotgun sequence.
ACCESSION   DASSMB010000131 DASSMB010000000
VERSION     DASSMB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 23327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23327
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1802357"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1700)
                     /gene="glgX"
                     /locus_tag="VFJ72_08010"
     CDS             complement(<1..1700)
                     /gene="glgX"
                     /locus_tag="VFJ72_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET7479441.1"
                     /translation="MSSTSKQKSVVWPGEPYPLGATWDGEGVNFALFSENAEKVELCL
                     FDPSGKKEVERIEVGEQTDQVWHCYLPYARLGQLYGYRVHGPYDPANGHRFNPNKLLM
                     DPYAKATVGPFDWSYPHFGYRLGAEDEDFTFDKRDNAPGMPKCRVVDTAFTWGEDARP
                     HTPWHDTIIYELHVKGFTQQHPDIPEALRGTYAGLASGPAIEHLKRLGVTAVELMPVH
                     QFVDDKHLLDQGLKNYWGYNSIGFLAPDMRYSATGSISEFKTMVKRLHSAGLEVILDV
                     VYNHTAEGNHLGPTLSFRGIDNAAYYRLTDDKRFYMDYTGTGNTLNMMHPRVLQLIMD
                     SLRYWILEMHVDGFRFDLASALARELHAVDRLGAFFDIIRQDPVISQVKLIAEPWDVG
                     EGGYQVGNFPVGWTEWNGKYRDEVRSYWKGDGGLVGELAYRLTGSSDLYERGGRRPYA
                     SINFITAHDGFTLRDLVSYEQKHNEANGEDNNDGESHNRSWNHGVEGPTDDPEIQAER
                     AKAQRNFLATLLLSQGVPMILHGDEMGRSQDGNNNTYAQDSEIAWMHWDTIDKPLVEF
                     T"
     gene            complement(1801..3981)
                     /gene="glgB"
                     /locus_tag="VFJ72_08015"
     CDS             complement(1801..3981)
                     /gene="glgB"
                     /locus_tag="VFJ72_08015"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET7479442.1"
                     /translation="MRHEREIQAVLNGEHSDPYGFLGMHEEDGGFVVRAFTPGASSVS
                     AVSEDGRALGELEKVHPDGFFAGPVATESRVPYRLRVEWEGGGGDEFEDPYRFPSTIG
                     ETDLYLFGEGSHLRLYEKFGAHVTELEGVRGVSFAVWAPNARRVSVIGDFNNWDGRKH
                     PMRKHPGGGVWEIFIPEIGEGEVYKYEIKGADGNLLPLKADPFGFRAEKLPGTASIVH
                     DPDRYEWGDTAWMENQIGKNSLDSPMAIYELHLGSWRRNDDGTYYSYEQLAEELVSYV
                     SEMGYTHVEFLPPTEYPFDGSWGYQPLGLFAPTSRFGTPDEFKKLVDEFHRAGIGVII
                     DWVPAHFPEDAHGLGFFDGTHLYEHADPRRGRHADWGTLVFNFGRNEVRNYLLANALF
                     WLAEYHVDGLRVDAVASMLYLDYSREEGEWVPNEFGGNEDLQAVAFLKRLNEVVYSEH
                     PGAATLAEESTAWPMVSRPTSMGGLGFGFKWNMGWMHDTLEYIQEDPIHRRYHHDKLT
                     FGLIYAFNENFVLPLSHDEVVHGKRSLIGKMPGDDWQKFANLRAYYGFMYGHPGKQLL
                     FMGGEFGQWREWNHDAGLDWHLLDNPMHSGVKTLLQDLNALYKKTPALHEVDFEPAGF
                     EWIEGGDRDQSVVSFLRRGKSPADFVVVVCNFTPVVRHGFRVGVPASGSYEEVLNTDA
                     TRYGGSGVENSGGMTAETIPANGREYSVPLDLPPLATVVLKPVS"
     gene            complement(3981..7385)
                     /gene="treS"
                     /locus_tag="VFJ72_08020"
     CDS             complement(3981..7385)
                     /gene="treS"
                     /locus_tag="VFJ72_08020"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET7479443.1"
                     /translation="MTEMQRSADGAIKRPARIHEKDPLWYKDAIIYHLHVKAFFDADN
                     NGIGDFKGLTQRLEYIRDLGVTAIWVMPFYPSPMRDDGYDISEYKNVHPDYGTKRDFR
                     AFVRKAHELGLRVITELVINHTSDQHPWFQRARRAPKGSAKRDFYVWSDTTDRYEDTR
                     IIFTDTERSNWSWDEEAGQYYWHRFFSHQPDLNHDNPQVFKAIMGVMRFWLDAGVDGL
                     RLDAIPYLIEREGTDNENLPETHEVLKKMRAEMDARYEERLFLAEANQWPEDVLPYFG
                     EGDECHMAFHFPLMPRIYMAVAQEDRHPIVEIMQQTPEIPDNCQWAVFLRNHDELTLE
                     MVTDRERDYMYQTYATDPRMRVNIGIRRRLAPLMENDRPRIELLNSLLMSMPGTPIIY
                     YGDELGMGDNVFLGDRDAVRTPMQWTSDRNAGFSRTDPARLYLPPIMDAVYGYESVNV
                     EAQSRSTGSLLNWMKRLISVRKAHKAFGRGTLEFLHPGNRKVLAYLREHEDEAILCVA
                     NLSRSAQPVELDLSRFRERIPVELIGGSSFPPIGSLPYFLTLPGHAFYWFRLSLETEA
                     SPPRWHEGSPRFLEPPVLVLPAGRETARRAHRAPLRIPSRQLSHLERDALPEFLKSRR
                     WFASKGAGIERVEVATRAEVGESHVLTLLRVHVRDDSGEEAEPHLYFLPLAAAWEEDG
                     EGDGLEEFMPNTLAKLRRRSKMGVLYDALMDEEFCRGLVAAMEENRDVAIGEGEIHFS
                     RTDVFDDLLGGESPSELAVRRAGVEQSNTSMIVGERLILKAYRRLQKGTNPDLEIGRY
                     LTEEVRFGNTPPLAGAIEYEGPDGEFTTLGLLQGFVSNQGDGWSYTLDYLNRYLEERA
                     VTAWTIEEDEEPEPEEPEADSAFFMGLIHTLGLRTGELHEAFATPTDDEAFAPEVASP
                     EEVAGWVQGVRDELKSTFNTLKRRQKKLPEEVRADVERLLEKRKEVEKRIRAVAGEEF
                     EVVKTRHHGDYHLGQVLVVNNDFQIIDFEGEPARPLAERRAKQCPLRDVAGMLRSFNY
                     AARSAFLRVSAERSEQHVEVLEAEVAAWERRAREAFMEGYAEGARGAASYPEDPEQAR
                     RLVELFTIEKALYEIRYELDNRPDWLGIPVRGILDLIEEPVETEQS"
     gene            complement(7385..9328)
                     /locus_tag="VFJ72_08025"
     CDS             complement(7385..9328)
                     /locus_tag="VFJ72_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET7479444.1"
                     /translation="MPGTYSVVIEGVYPEVNCGRYPVKREVGDRFEVRADIFKDGHDV
                     LSAVIKYREKGTRRWNTTPMAQINPGLDLWSGTFDLAKNTRYEYTIEAWFDEFETWRR
                     DIGKKVDDGQVVDLELIEGRQIVEATQARSKDKRLAQTLAGFDASGYEGQLALLRSPE
                     VRELMRAYPDRSMSTTYKTLEVTVDRERARYGAWYEMFQRSQGTVPNQSATFADCERR
                     LPEIRRMGFDVVYLLPVHPIGHTHRKGRNNALVAAPGDPGSPYAIGSEEGGHKSVNPE
                     LGTLEDFRSFVEATREYGMEVAIDFAIQCSPDHPYIKEHPEWFKFRPDGSIKYAENPP
                     KKYQDIVNVDFYSEDWENLWREWRDVILFWVEQGVKIFRVDNPHTKSFAFWEWCIKEV
                     QDEHPDVIFLAEAFSRPRIMQNLAKLGFTQSYTYYTWRNEKWELIEYLTELSQQESKE
                     YMRPNFFANTHDILPHILQFGGRPAFQMRFALAATLSPTYGIYNGYELCENTPRGERG
                     TTVYYQDSEVYEYKVWDWDRPGNIKDYIGRINQIRRENPALHYLKNLSFHEAPNDNIL
                     FYGKQKDGNAVFTTVNLDPHSPHDTHVQFPLWQLGISEDEEYVVEELISERRMRATGS
                     WFWVRLTPWSNPVEIFRVTKVGA"
     gene            9491..9652
                     /locus_tag="VFJ72_08030"
     CDS             9491..9652
                     /locus_tag="VFJ72_08030"
                     /inference="COORDINATES: protein motif:HMM:NF045043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479445.1"
                     /translation="MSDEVRDKQGDAEPLNWACTNCGYRVEGEPPPEKCPDCGADREM
                     FDQVPKPGF"
     gene            9737..10330
                     /locus_tag="VFJ72_08035"
     CDS             9737..10330
                     /locus_tag="VFJ72_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479446.1"
                     /translation="MDVATIAQIVTSVTNTLFVVVLAAGYYYTWKSSQSTLREMQAQR
                     TAMGRPLVIVQEDYDSLPELDITIRNVSEGAARDITFEFSAPIENSSGSVISEMPYFD
                     NGLDFLPPNGEISCYWDELDSLLPFLDKKGLREGIRVTVRYKDLAGASYESSWKLDPF
                     LYKDTGLVKRKGMADLVRAVEKLDARDGQENAPGSAG"
     gene            10426..11511
                     /locus_tag="VFJ72_08040"
     CDS             10426..11511
                     /locus_tag="VFJ72_08040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7479447.1"
                     /translation="MSDTELFARATLDSLSAHIAIIDGSGEIVAVNKAWREFAAANNA
                     TDNVSEGVNYLHVCDSAVGDQSGYAAAFAEGIRAVLRGEREEFAMEYPCHSPSEHRWF
                     IGRVTLFRNNTPQAVVAHENITQRKLAEEALASVLRTRTDFMADVSHELRTPLTVIRG
                     NAEVGMQRVLDPTHREMLEEILQETERMTRMVEDLLLLARSDSGSLPLTLEPMPIATL
                     LLRLSRRAHALARERGASLRTAMTGEGRLRCDVERIEQAVLVLVDNAAKYGPPEVEIT
                     LSSRTRQGELLIEVADRGPGIRQEELPRIFERFYRGESPPEERGSGLGLPIAKTIIEA
                     HGGYVEAVSRVGEGTRMSIYLPLLGDS"
     gene            complement(11528..12043)
                     /locus_tag="VFJ72_08045"
     CDS             complement(11528..12043)
                     /locus_tag="VFJ72_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7479448.1"
                     /translation="MTTPLKDIPIFSRLDAEERAELEEILEPVSFSAGETIFEEGGPE
                     DYLYVVCSGIVEVHKKVLPGHRHVLATVSAPTVVGEMGLLTEPRAAATVTARTNIEAH
                     GINRDRFLEMLDGDSLAACKVVYEIGRTLSERMAKTDESIAHIIARIERADTSHDLDV
                     FQDTLLNEWSF"
     gene            complement(12059..12583)
                     /locus_tag="VFJ72_08050"
     CDS             complement(12059..12583)
                     /locus_tag="VFJ72_08050"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7479449.1"
                     /translation="MTENQLRTVRLFEFLTDEERAEFMEEAEAATFEVGEDIIREGGR
                     PGSLFVLTSGRVEVRKSLPGDRERVLAKIDAGVERTVVGERGLLGKSGASATVRASSR
                     VEAIRIPRETFRRMISEGRPAAFKLSYRIARVLAQRLTRLDEEVAEAIRELDRKGETD
                     LEAFRDRLITDWAI"
     gene            complement(12804..13175)
                     /locus_tag="VFJ72_08055"
     CDS             complement(12804..13175)
                     /locus_tag="VFJ72_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7479450.1"
                     /translation="MKSFLKMLFVAGVFAGVSVVSLAVVQEDAQAEPVEASYYGYELA
                     GSLTASGQPFDPTALTAAHKTLPLGTQLLVTYGGNSVVVTVNDRGPYVAGRDLDLSQA
                     AAEQIGLTAAGSGVVDMQVIE"
     gene            complement(13400..13837)
                     /locus_tag="VFJ72_08060"
     CDS             complement(13400..13837)
                     /locus_tag="VFJ72_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479451.1"
                     /translation="MFGRLRGIFGGRREDTAPASSGLSSLAPEETAKIGTEEDPLRGY
                     EAAVERHEEAARAEQTGDPERAIALYEKSVAERFVGSHPYEALARLHERRRNHADALR
                     VTESYIDLARSGKMPRGAQRSADRKLPEFEARAERYRRSTSME"
     gene            13948..15729
                     /locus_tag="VFJ72_08065"
     CDS             13948..15729
                     /locus_tag="VFJ72_08065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7479452.1"
                     /translation="MPHEFARYLKVRSAYGATWSRDGRRLAFLTDITGVPQVWEVPAE
                     GGWPEQLTFHEERVMGAEYSPAGDRIVFSMDTGGNERAQIFLLDPSAGEECDLTNAPD
                     AIHYFGGHSPDGARIAYTATRRNGTDFDVFVQDLPDGEPQCVWEVSGYHTVADWGPDG
                     SFLIVSRHHSNLNNDLYRLDLANASGEPTLLTPHDGDARFQSVNVTPDGASAYLATDR
                     DGDFLRLGRLDLSTLDLTYLTPDDWDVEEVKLSRDGRYMVASRNVDGYTEVRFFNGRG
                     SRRPGPQLPKGILGGFEFAPDSERLAFTVTAPERNPDVWLLDLPEGEPRRLTRSSTAG
                     IPPRTFRRPRLVRYPTFDDGQIPALFHEPDAGSAPVIVNVHGGPESQSRPGFAPVTQY
                     LIHRGYAVFAPNVRGSTGYGKEYTHLDDVELRMDSVKDLAHAAHWLREQGHERVAVMG
                     GSYGGFMVLAALTEYPDLWSAGVDIVGIANMVTFLENTGSYRRRLREPEYGSLEKNRE
                     FLESISPIHKAEKITAPLMVVHGKNDPRVPVGEAEQIVERVKNNGGAVEYLLYEDEGH
                     GLAKLKNRLDAYPRIAAFLDRHLGPSA"
     gene            15813..18401
                     /locus_tag="VFJ72_08070"
     CDS             15813..18401
                     /locus_tag="VFJ72_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HET7479453.1"
                     /translation="MSALGRRWGRGVVFGLLLVLATVIPAAAQVAPGDGKSPTLSTTD
                     RLDDRRYVTTGDRAYIVGTEAGRFPAMGFHTRGEMGGVWSPPIKLLDGIWFGIDDQWI
                     GPATKFTSGYGHVNMNLPGRTGLRIGRTDFVPDGKRAVLVGLKFSAGDTRQSFTLKMD
                     AHSELMGAYPWGETTPNQLTFNLADGVAVEDGKLVFREQGTPPVANAEAHDWAAVVGS
                     SLTPTGSDAGADFRGPQDPAVICPASGPTAPPAPPRCDDTEYGKGKGGELRYNVSVPA
                     NSSRTVWFAVSGSESGAAAAKATNASVLGDPEGALDKKVTGRLALQEKSKLSLPGDRR
                     LQRSIDWSKQNLADSVQSARDLEIRETNAGKNYPPPEGTLDSMRFLGAGFPDYPWLFG
                     TDGEFTAFASVGVGQFGPIKDHMRALKRVSEIDNGNSGKVVHEVVTDGSVFFGSNADA
                     GNTDETAKFPSAVALIWRWTGDDAFRDEMYPFTVKNMRYIFRVLDKDNDGWPEGLGNV
                     EREGMGEEKLDNTVYTIRGLYDLADMARSKGDKATANWAEDKAATMRARFEKAWWFGR
                     EGAAQYADSLDDPGNRQVFQRHWIGVTPMDVDLVDKSDRTVPGLASRPHGQAALAERE
                     LACYTDRFGMYHTGTGPTSAAGGNKGATCDTHVSEVQSERTIFTLNTAIMAVGEGNYG
                     RLGTDQQKHYTKANADLQLIPDEQPGAMPEIAPSPDYGRSIDRPFNERAMVLQAWGAY
                     GTIWPVLHQQLGIRPDMGRGELAVVPQVPPGSPGLSGENIRLVKGSVAVSADHEGKTY
                     TTKVSTRLKVDLTIGHTVPRDAKVSSVRLNGDKVRYKVRTTNRGKEVLVKAPKTGKQE
                     LVIRAG"
     gene            18560..20089
                     /locus_tag="VFJ72_08075"
     CDS             18560..20089
                     /locus_tag="VFJ72_08075"
                     /inference="COORDINATES: protein motif:HMM:NF017591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase PhoX"
                     /protein_id="HET7479454.1"
                     /translation="MSISGNWDRRVSRRAFLGMGGMSAAALVLGSREAMAKKGSTAGF
                     GELVADPGGLIDLPKGFQYRVISAKGSPLSSGGTVPGDPDGMAAFAGPKKGTALLVRN
                     HELKPEDGSPVVGRNPYDPSQTGGTTGILVNLEDRTEIKDFVTSSGTRNNCAGGATPW
                     GTWLTCEEDRTTGHGYVFEVDPRDPENDLSKTPIRAMGFFSHEAVDIDPATGIAYLTE
                     DDFRGAVTPDPNQEVVADYSTSEIDPSTGQRGTGTRVSFLYRYIPDDRSQKPGALQMG
                     GRLQALALEEKPNYNVDLASPGQKFGVVWKDVNPEEPHESAEDAGAARFGRLEGAYFA
                     GGAFWFDDTIGGEKRLGQVFRYLPATETLELFYEGTEPGKMESPDNIVVTPWGDLWFA
                     EDEAVDGDSVNRVMGITPEGQVYVFARNATSDSEFAGPTFSPDGKTFFVNMQGDGLTF
                     AIWGPFGRHNSGRQRQMAVAAPPKSMAPKVSGELAEAADKFGMSHLEAAAYERLGVSL
                     A"
     gene            20311..21981
                     /locus_tag="VFJ72_08080"
     CDS             20311..21981
                     /locus_tag="VFJ72_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7479455.1"
                     /translation="MASLLLSFAVALMLAATMIWTAGAQSKPNPKPVPEDRIADVQLL
                     GVNDFHGNLEPPRTLNGRAVGGAAYLDAYMDKYAAANPERTIRVHAGDMVGASPLISS
                     YFHDEPTIYAANQMGFDVGTVGNHEFDEGGEEMLRLINGGQRDDGKQFKDGVDGEPVN
                     TSDPDFPGADFPYIAANTVYKDTGKSVLPPYQVVKRDGVKVGFIGVTTLETPNIVVPD
                     AVAPFDYLDISDTVNKYAKQLQDKGVQTIVVLAHSGGNQVSATEATGEILDETSQMSN
                     AVDVVVAGHSHSLLNNRVGNKLVVEAFSFGTAFDAVDMKIDRKTGDVTSSSADIVTTY
                     DDAIQPDAKTAELVADYKARIAPISERVVGTAQTDISRTSNVAGESAMGDLIADAQRD
                     FAGADFAFMNPGGIRADIAAGEVTYGELFTVQPFDNQVVSMDLTGDQIYRLLEQQFPP
                     NQVAGTKRILQVSGLKYTYDESLPEGQRITSLTKTDGTPIDRATTYKVAANSFIATGG
                     DRFTVLKEGQNVQTIGGDLDALEAYIDSLQQPFSAPDPAGEQRITKAG"
     gene            22138..>23327
                     /locus_tag="VFJ72_08085"
     CDS             22138..>23327
                     /locus_tag="VFJ72_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HET7479456.1"
                     /translation="METTKFLLDEKQIPESWYNLVPDLPFELEPPLNPATREPVGPEA
                     FAPIFPQEIIRQEVTTDSYVEIPEEVREIYSLWRPTPLFRARRLEKFLDTPAHIYYKY
                     EGVSPSGSHKPNTAVPQAYYNREEGVRRLTTETGAGQWGSSLAFACRQMGLECTVYMV
                     RVSYDQKPYRRIMMETYGAEVHASPTDLTNAGRNILEEHPDSPGSLGIAISEAVEDAV
                     MHDDAKYSLGSVLNHVLLHQTVIGQEALQQMDLAGEYPDIVVGCVGGGSNFGGVAFPF
                     IRENLKNGRDTRIVAVEPASCPTLTKGRYTYDFGDTAGTTPMMKMYTLGHSFVPSGIH
                     AGGLRYHGDSPIVSALVHEGLVEARAYAQNPTFEAGVTFARTEGIVPAPEVTHAIKAT
                     IDLAL"
BASE COUNT         4139 a         7642 c         7531 g         4015 t
ORIGIN      
        1 gtgaactcga ccagcggctt gtcgatggtg tcccagtgca tccaggcgat ctcagagtcc
       61 tgggcgtaag tgttgttgtt gccgtcctgg ctgcggccca tctcgtcgcc gtgcaggatc
      121 atcggcacgc cttggctgag cagcagggtg gcgaggaaat tgcgctgcgc cttggcgcgc
      181 tcggcctgga tctccgggtc gtcggtcggc ccctcgacgc cgtggttcca cgagcggttg
      241 tggctctcgc cgtcgttgtt gtcctcgccg ttggcctcgt tgtgcttctg ctcgtaggag
      301 acgaggtcgc gcagggtgaa gccgtcgtgg gcggtgatga agttgatgga ggcgtagggc
      361 cggcggccgc cgcgctcgta gaggtcgctt gagccggtga gccggtaggc cagctctccg
      421 acgagcccgc cgtcgccctt ccagtaggag cggacctcgt cgcggtactt gccgttccac
      481 tccgtccaac cgaccgggaa gttcccgacc tggtagccgc cctcgccgac atcccacggc
      541 tcggcgatga gcttgacctg cgagatcacc gggtcctgcc ggatgatgtc gaagaacgcc
      601 ccgagccggt cgacggcatg caactccctc gcgagcgcgc tcgccaggtc gaagcggaaa
      661 ccgtccacgt gcatctcaag gatccagtac cggagcgagt ccatgatgag ctgcaacact
      721 ctcgggtgca tcatgttgag ggtgttgccg gtcccggtgt agtccatgta gaagcgcttg
      781 tcgtccgtga gccggtagta ggcggcgttg tctatgcccc ggaacgagag cgtcggcccg
      841 aggtggtttc cctcggcggt gtggttgtag acgacgtcga ggatgacctc caggccggcg
      901 gagtggagcc tcttcaccat cgtcttgaac tccgagatgc tcccggtggc cgagtagcgc
      961 atgtccgggg cgaggaagcc gatggagttg tagccccagt agttcttgag cccctggtcc
     1021 aggaggtgtt tgtcatccac gaactggtgg accggcataa gctccaccgc cgtcaccccg
     1081 agccgcttga gatgctctat ggccgggccg gaggcgagcc cggcgtaggt gccgcgcagg
     1141 gcctccggta tgtcggggtg ctgctgcgta aagcccttga cgtgcagctc gtagatgatc
     1201 gtgtcgtgcc agggggtgtg cgggcgcgca tcttcgcccc aggtaaacgc ggtatccacg
     1261 acccggcact tgggcatgcc gggggcgttg tcgcgcttgt cgaaggtgaa gtcctcgtcc
     1321 tcggcgccga gcctgtatcc gaagtgcggg tagctccagt cgaagggtcc cacggtcgcc
     1381 ttcgcgtagg ggtccatgag gagcttgttc gggttaaaac ggtgtccgtt agcggggtcg
     1441 tagggaccgt ggacgcggta gccgtagagc tgaccgaggc gggcgtacgg gaggtagcag
     1501 tgccagacct gatccgtctg ttccccgacc tcgatccgct cgacttcctt ctttcccgag
     1561 gggtcgaata gacacagctc caccttctcc gcgttctccg agaacagcgc gaagttcaca
     1621 ccctccccgt cccaggtcgc ccccagggga tacggctctc ccggccacac cacactcttc
     1681 tgtttgctag tactgctcaa ggcagggact ttcctttctc tgggagctct cagcattcag
     1741 gcatcgagct taaagcatcg ccttttgttt cttgtcttca agctgccggc tgaccgctga
     1801 ttagctgact ggctttaaaa caaccgtcgc cagcggcggt aagtccagcg ggacggagta
     1861 ttcccgtccg ttggccggaa tagtctcggc agtcatgcct ccgctatttt cgacgccact
     1921 accgccgtag cgggtggcgt cggtgttgag gacctcttcg taggagccgc tggccggaac
     1981 gccgacccgg aacccgtgac gcacgaccgg ggtgaagttg cagacaacga ctacgaagtc
     2041 tgccgggctc tttccccggc gaaggaagga taccacgctc tggtccctgt ccccgccctc
     2101 gatccactcg aaccccgccg gctcgaagtc gacctcgtgc agcgcgggcg tcttcttgta
     2161 gagcgcgttg aggtcctgca gcagcgtctt cacgccggag tgcatggggt tgtccagcag
     2221 gtgccagtcg agccccgcgt cgtggttcca ctcgcgccac tgaccgaact cgccgcccat
     2281 gaacaggagt tgcttgccgg ggtggccgta catgaagccg tagtaggcgc gcaggttggc
     2341 gaacttctgc cagtcgtcgc cgggcatctt gcctatgagc gagcgcttgc cgtggacgac
     2401 ctcgtcgtgg gagagcggca gcacgaagtt ctcgttgaag gcgtagatga ggccgaaggt
     2461 gagcttgtcg tggtggtaac ggcgatggat agggtcttcc tggatgtatt cgagcgtgtc
     2521 gtgcatccag cccatgttcc acttgaagcc gaagccgagc cctcccatcg aggtcgggcg
     2581 gctgaccatc ggccacgccg tcgactcctc cgcgagcgtc gccgcgccgg gatgttcgga
     2641 gtagacgacc tcgtttagcc gtttgaggaa cgcgacggcc tgtaggtcct cgttgccgcc
     2701 gaactcgttg gggacccact cgccctcctc gcgcgagtaa tcgaggtaga gcatggaggc
     2761 gacggcgtca acgcgcagtc cgtccacgtg gtactcggcg agccagaaga gggcattggc
     2821 gagcaggtag ttacgcacct cgttgcgccc gaagttaaag acgagcgtac cccagtcggc
     2881 gtggcggcct ctacgggggt cggcgtgctc atagaggtgg gtgccgtcga agaagccgag
     2941 gccgtgggcg tcctccggga agtgggccgg gacccagtct atgatgaccc cgatccccgc
     3001 ccgatggaac tcgtccacga gcttcttgaa ctcgtccggc gtgccgaacc ggctcgtcgg
     3061 ggcgaagagc ccgagcggct gataacccca ggagccgtcg aacgggtact ccgtcggcgg
     3121 caggaactcg acgtgcgtgt agcccatctc cgagacgtaa gagaccagtt cctcagccag
     3181 ttgctcatag gagtagtagg tcccgtcgtc gttgcggcgc caggagccga ggtgcagctc
     3241 gtagatcgcc atcggcgagt cgagggagtt cttgccgatc tggttctcca tccaggcggt
     3301 gtcgccccac tcgtagcggt ccgggtcgtg gacgatggag gcggtgccgg ggagcttctc
     3361 ggcccggaaa ccgaacgggt cggccttcag agggagcagg ttgccgtcgg cgcccttgat
     3421 ctcgtacttg tagacctcgc cctcgcctat ctcggggatg aagatctccc acaccccgcc
     3481 gccgggatgc ttgcgcatcg ggtgcttgcg gccgtcccag ttgttgaagt ccccgatcac
     3541 cgacacccgc cgggcgttcg gcgcccacac ggcgaagctc acgccccgga cgccctccag
     3601 ctccgtcacg tgcgccccga acttctcgta gagcctgagg tggctgccct cgccgaacag
     3661 atacaggtcc gtctcgccga tggtcgaggg gaagcggtag gggtcctcga actcgtctcc
     3721 gcccccgccc tcccactcca cgcggaggcg gtacggcacg cggctctcgg tcgcaacggg
     3781 accggcgaag aacccgtcgg gatggacctt ctccagctct cccagggcgc gaccatcctc
     3841 tgagacggcg ctcacggatg aggcgcccgg ggtgaaggca cgcaccacga agccgccgtc
     3901 ctcctcgtgc atgccgagga agccgtaggg gtcggaatgt tcgccgttca agacggcctg
     3961 gatctctctc tcgtgtctca tcagctctgc tccgtctcta ccggctcctc gatgaggtcc
     4021 aggatcccgc gcaccgggat acccaaccag tcggggcggt tgtctagctc gtaccggatc
     4081 tcgtacagcg ccttctcgat ggtgaacaac tcgaccagcc gccgcgcctg ctcggggtcc
     4141 tccgggtaag acgccgcgcc gcgagcgccc tcggcatagc cctccatgaa cgcctcgcgc
     4201 gcccgccgtt cccaggccgc aacttcagcc tccagcacct ccacgtgctg ctccgagcgc
     4261 tcggcgctca cccgcaggaa agcggaccgc gcggcgtagt tgaaagaccg gagcatcccc
     4321 gccacgtccc gcaaggggca ctgcttcgcg cgccgctcgg cgaggggccg ggctggctct
     4381 ccctcgaagt ctatgatctg gaagtcgttg ttcacgacca gaacctgtcc gaggtggtag
     4441 tcgccgtgat gccgggtctt gaccacctcg aactcctccc cggcgacggc cctgatgcgc
     4501 ttctcgacct ccttgcgctt ctccagcagt cgctccacgt ccgcgcgcac ctcctccggc
     4561 agcttcttct gccgccgctt gagagtgttg aaggtgctct tcaactcgtc gcgcacaccc
     4621 tgcacccagc ccgcgacctc ctccggcgag gcgacttcgg gggcgaaagc ctcgtcatcg
     4681 gtgggcgtgg caaaagcctc atgcaactca ccggtgcgga gtcccagagt gtgtatcagg
     4741 cccatgaaga acgcgctgtc ggcctcgggc tcctcgggct ccggctcttc gtcctcttcg
     4801 atggtccagg cggtaacggc ccgctcctcc aggtagcggt tcaggtagtc cagagtgtag
     4861 gaccagccgt cgccctggtt ggagacgaag ccctggagga ggccgagggt ggtgaactca
     4921 ccgtcgggtc cctcgtactc gatggctccg gccagcggcg gggtgttgcc gaagcggacc
     4981 tcctcggtca ggtagcgtcc gatctccagg tccgggttcg tgcctttctg caggcggcgg
     5041 tatgccttca ggatcagacg ctcaccgacg atcatggacg tgttgctctg ctccaccccg
     5101 gcgcgccgca cggcaagctc cgacggcgat tctcctccga gcaggtcatc gaagacatcg
     5161 gtccttgaga agtggatctc cccctccccg atggcgacgt cccggttctc ctccatcgcc
     5221 gccacgagcc cccggcagaa ctcctcgtcc atgagagcgt cgtagagcac gcccatcttc
     5281 gagcgccggc gcagcttggc gagggtgttg ggcatgaact cttccaggcc gtctccctca
     5341 ccgtcctcct cccaggccgc cgcgagcggc aggaagtaga ggtgcggctc ggcctcttcg
     5401 cccgagtcgt cccggacgtg gacccggagc agcgtcagga cgtgagactc cccgacctcc
     5461 gcgcgcgtcg ccacttcgac acgttcgatc cccgccccct tggaggcgaa ccagcgccgg
     5521 gacttcaaga actccggcag ggcgtcgcgt tccaggtgag agagctggcg cgacggtatc
     5581 cgcagcggcg cccggtgcgc ccggcgcgcc gtctcccgcc cggcgggcaa caccaatacc
     5641 ggcggctcca gaaagcgcgg gctcccctcg tgccagcggg gcggcgaagc ctcggtctcc
     5701 agtgagaggc ggaaccagta gaaagcgtgt cccggcaggg tcaggaagta gggaaggctg
     5761 cctatcgggg ggaagctgct gccgcctata agctctaccg gtatccgctc ccggaagcgc
     5821 gagaggtcca gctcgaccgg ctgggccgag cgactaaggt tggcgacgca caggatcgcc
     5881 tcgtcctcat gctcgcgcag ataggccagc actttacggt tgccgggatg caggaactcc
     5941 agcgtgcccc gcccgaatgc cttgtgggcc ttgcggacgg agataagccg cttcatccag
     6001 ttcaggagcg agcccgtgct gcggctctgg gcctcgacgt tcaccgactc gtagccgtag
     6061 accgcgtcca tgatcggggg caggtacagc cgggcggggt cggtgcgcga gaagccggcg
     6121 ttccggtcgg aggtccactg catcggggtg cggacggcgt cccggtcgcc gaggaagacg
     6181 ttatctccca tcccaagctc gtcgccgtag tagatgatcg gggtccccgg catgctcata
     6241 agtaagctgt tgagtagctc tatgcggggc cggtcgttct ccatgagcgg cgcgaggcgg
     6301 cggcggatgc cgatgttgac gcgcatccgg gggtcggtcg cgtaggtctg gtacatgtag
     6361 tcccgctcgc ggtcggtgac catttcgagg gtgagctcgt catggttgcg aaggaatacc
     6421 gcccactggc agttgtccgg gatctcgggg gtctgctgca tgatctcgac tatcgggtgc
     6481 cggtcttcct gcgcgacggc catgtagatc ctgggcatga gcgggaagtg gaaggccatg
     6541 tggcactcgt cgccctcccc gaagtacggc agcacgtcct cgggccactg gttggcctcg
     6601 gcgaggaaga gccgctcctc gtagcgtgcg tccatctcgg cgcgcatctt cttgagaacc
     6661 tcgtgggtct ccggcaggtt ctcgttgtcc gtcccctcgc gttcgatcag gtacgggatc
     6721 gcatcgagcc gcaggccatc aacgccggcg tcgagccaga agcgcatcac gcccatgatg
     6781 gccttgaaga cctgcgggtt gtcgtggttg aggtccggct ggtgggagaa gaaacggtgc
     6841 cagtagtact gtccggcctc ctcgtcccag gaccagttgg accgctccgt atcggtaaag
     6901 atgatgcggg tgtcctcgta gcggtcggta gtgtcgctcc acacgtagaa gtcgcgcttc
     6961 gccgatccct tcggcgcccg ccgggcgcgc tggaaccagg ggtgctggtc ggaggtgtgg
     7021 ttgatgacca gctcggttat gaccctgagg ccgagctcgt gggccttgcg gacgaaggcg
     7081 cggaagtccc gcttggtgcc gtagtcgggg tggacgttct tgtactcgga gatgtcatag
     7141 ccgtcgtcgc gcatcggcga ggggtagaag ggcattaccc agatcgcggt tacgcccaaa
     7201 tcccttatgt actccagcct ctgcgtgagg cccttgaagt ccccgatgcc gttgttgtcc
     7261 gcgtcgaaga acgcctttac gtgcaggtgg tagatgatgg cgtccttgta ccagagcggg
     7321 tccttctcat ggatgcgcgc cggcctcttt atcgccccat ccgcggaccg ctgcatctcg
     7381 gtcatcaggc ccccactttc gttacgcgga agatctccac ggggttgctc cagggcgtga
     7441 gccgcaccca gaaccagctc cccgtggccc gcatccggcg ctccgagatg agctcctcca
     7501 ccacgtactc ctcgtcctcg ctgatcccga gctgccacag cgggaactgc acgtgcgtgt
     7561 cgtgcgggct gtgcgggtcg aggttgacgg tggtgaagac ggcgttgccg tccttctgct
     7621 tgccgtagaa caggatgttg tcgttcgggg cctcgtggaa ggagaggttc ttcaggtaat
     7681 ggagggcggg attctcgcga cggatctggt ttatacgtcc tatgtagtcc ttgatattgc
     7741 cgggcctgtc ccagtcccag accttgtact cgtagacctc ggagtcctgg tagtagacgg
     7801 tcgtgccgcg ctcgccccgc ggggtgttct cgcacagctc gtagccgtta tagatgccgt
     7861 aggtcgggga gagggtggcg gcaagcgcga agcgcatctg gaaggcgggc cggccgccga
     7921 actggaggat gtgcggcagg atgtcgtggg tgttggcgaa gaagttcggg cgcatgtact
     7981 ccttcgactc ctgctgcgag agctcggtga ggtactcgat caactcccac ttctcattgc
     8041 gccaggtgta gtaggtgtag gactgcgtaa agccgagctt ggcgaggttc tgcatgatgc
     8101 ggggccggga gaaggcttcg gccaggaaga tcacatccgg gtgctcgtcc tgaacctcct
     8161 tgatgcacca ctcccagaac gcgaaggact tggtgtgcgg gttatccacc cggaatatct
     8221 tcacgccctg ctcgacccag aacaggatca cgtctcgcca ctctcgccac aggttctccc
     8281 agtcctcgga gtagaagtcc acgttgacga tgtcctgata cttcttcggc gggttctcgg
     8341 cgtactttat gctcccgtcg ggccggaact tgaaccactc ggggtgctct tttatgtacg
     8401 ggtggtccgg cgagcactgg atggcgaagt ctatggcgac ctccatcccg tactcgcgcg
     8461 tcgcctccac gaacgagcgg aagtcctcca gcgtcccgag ctccgggttg acggacttgt
     8521 gcccgccctc ctcgctcccg atggcgtagg gactgccggg gtcccccggc gcggcgacga
     8581 gggcgttgtt gcggcccttg cggtgcgtgt gtccgatagg atggaccggg aggaggtaga
     8641 cgacgtcgaa gcccatgcgc cggatctccg gtagccgccg ctcgcagtcg gcgaacgtgg
     8701 cgctctggtt cggcacggtc ccctgggagc gctggaacat ctcgtaccac gccccgtagc
     8761 gcgcccgctc gcggtccacg gttacctcaa gcgtcttgta ggtggtggac atcgagcggt
     8821 ccgggtacgc ccgcatcagc tcgcgcacct ccggggagcg cagcagggcg agctggccct
     8881 cgtagcccga ggcgtcgaag ccggcgaggg tctgcgcgag gcgcttgtcc ttcgagcggg
     8941 cctgggtcgc ctcgacgatc tggcggccct cgatcagctc caggtccacc acctggccgt
     9001 cgtccacctt cttgccgatg tcccgccgcc acgtctcgaa ctcgtcgaac catgcctcga
     9061 tggtgtactc gtagcgcgtg ttcttcgcaa ggtcgaacgt gccgctccag aggtcgaggc
     9121 cggggttgat ctgtgccatc ggcgtcgtgt tccagcggcg cgttcccttc tcccggtact
     9181 tgatgacggc ggagagcacg tcatggccgt ccttgaagat gtccgcccgc acctcgaaac
     9241 ggtcgccgac ctcccgtttc accggatagc gtccgcagtt cacctccgga tatacgcctt
     9301 ctataacaac gctataagtc cccggcacct cgtgtcctcc ataaatcgct caacctcttg
     9361 cgttaaccgc ctaggatact cgtatatcta catgattaaa gcggacccgc aaaaccgcgg
     9421 caagcggtct tatggaaccg cgcttcgggt aagatgcctc cgaggcggat attcaggata
     9481 ggagcacagc atgagcgacg aagtacgaga caaacagggc gacgccgagc ctctgaactg
     9541 ggcctgcact aactgcggct accgcgtcga gggcgagccg ccacccgaga aatgtcccga
     9601 ctgcggcgcg gaccgggaga tgttcgatca ggtccccaaa ccgggattct aagaagcttc
     9661 tcaatctgta agccagtcgg cacgttagcc agagcaagcg ctgaaaacca gaccgcttac
     9721 ggatagtcta cggctcatgg acgtagcgac catcgcgcag atcgttacct cggtgaccaa
     9781 cacgctcttc gtggtcgtgc tggccgccgg ctactattac acctggaagt ctagccagag
     9841 cacgctgcgc gagatgcagg cgcagcgcac cgctatgggc cggccgctgg tcatcgtgca
     9901 ggaggactac gacagcctgc cggagttgga catcacgatc cgaaacgtca gcgagggcgc
     9961 ggccagggac ataaccttcg agttctccgc gccgatagag aactccagcg gttccgtcat
    10021 ctccgagatg ccgtacttcg ataatgggct ggacttcctg ccgccgaacg gtgagatctc
    10081 ctgctactgg gacgagctgg actctctgct gcctttcctg gacaagaaag gtctccgcga
    10141 aggcatccgc gttaccgtcc gctacaagga cctcgccgga gcctcctacg agagtagttg
    10201 gaagctcgat ccgttcctgt acaaggacac cggcctcgtc aagcgcaagg gcatggccga
    10261 cctcgtgcgc gccgtggaga agctcgacgc gagggacggg caagagaacg cgcccggctc
    10321 tgcgggatga aggtccctcg tctctggggt tgctgaaccg gggccgtgtt aagatgcccg
    10381 gcagggtagt agggtacggt ttgggtttag gtggacgagg tagccttgag cgataccgag
    10441 cttttcgcac gcgccaccct ggatagcctt tcggcgcaca tagccattat cgacggatcg
    10501 ggcgagatcg tcgcggtcaa caaggcctgg agagagttcg ccgcggcgaa caacgccacg
    10561 gacaacgttt cggagggcgt caactatctc cacgtgtgcg attcggcggt cggcgaccag
    10621 tctgggtacg ctgccgcatt cgcggaaggt atccgcgccg tactccgtgg cgagcgcgag
    10681 gagttcgcga tggagtaccc gtgccactcc ccttccgagc accgctggtt catcggacgg
    10741 gtgacgctct tccgaaacaa tactcctcag gcggtagtcg cgcacgagaa tattacccag
    10801 cgcaagctgg cggaagaggc gctcgccagt gtcttgagga ccaggacgga ctttatggcc
    10861 gatgtctcgc acgagctgag gacccccctg acggtgatcc ggggcaacgc cgaagtcggg
    10921 atgcagcgag tgttggatcc cacccaccgg gagatgctgg aggagatcct tcaggagacg
    10981 gaacgcatga cccggatggt cgaggacctg ctgctcctgg cgcgctcgga ctccgggtcg
    11041 cttcccctga ccctggagcc gatgcccatc gctacgctac tgctccggct gtcccgtcgg
    11101 gcgcacgcgc tggcgcgaga gcgcggcgca tccctgcgta cggctatgac gggcgaggga
    11161 cgcctgaggt gcgacgtaga gcggatagag caggcggtgc tcgttctggt cgacaacgcg
    11221 gccaagtacg gcccacccga agtagagata acactctcct cgcggacccg gcagggcgag
    11281 ttgctgatcg aagtcgcgga ccggggaccc ggcatccggc aagaggagct gccacggatc
    11341 ttcgagcgct tctaccgggg cgagagccct cccgaagagc gcggttcggg gctgggattg
    11401 ccgatcgcca agactatcat cgaagcccac ggcggctacg ttgaagccgt gagccgcgtg
    11461 ggcgaaggaa cgcggatgtc catctatctc ccgctgctcg gagactcgta gtcatccggt
    11521 tcggatgcta gaagctccac tcgttgagga gtgtgtcctg gaagacatcg aggtcgtggc
    11581 tggtatcggc tctctctatc cgggcgatga tatgcgctat tgactcgtcg gtcttcgcca
    11641 tgcgctcgct caaggtgcgc ccgatctcgt agacgacctt gcaggcggcg agggaatcgc
    11701 cgtcgagcat ctccaggaag cggtcccggt ttatgccgtg cgcctctatg ttggtcctgg
    11761 ccgtgacggt cgccgcggcg cgcggttcgg tcaggaggcc catctcgccc acgacagtcg
    11821 gagcgctaac ggtagcgagc acatggcgat gtccgggcag caccttcttg tgtacctcca
    11881 cgatgccgga gcacacgaca tacaggtagt cctccggtcc gccctcctcg aagatcgtct
    11941 ctccggcgct gaagcttacg ggctccagga tctcctccag ctcggcccgc tcctcggcgt
    12001 ccaggcggct gaagatcggg atgtccttga ggggtgtggt catctctata taactccctt
    12061 agatcgccca gtcggtgatc agcctgtcgc ggaatgcttc aaggtcggtc tcgcccttgc
    12121 ggtcaagctc ccggatcgcc tccgccacct cttcgtcgag gcgggtaagc ctctgagcca
    12181 ggacgcgggc tatacggtag gagagcttga atgcggcggg ccgtccctcg gagatcatac
    12241 gccggaaggt ctcgcgcggg atccggatcg cctctacccg gctcgacgcc cgcacagtcg
    12301 ccgacgcccc gctcttcccc agcaaccccc gctcacccac gaccgtccgc tccaccccgg
    12361 cgtctatctt cgccagcacc ctctcccggt ctccgggcag gctcttcctc acctcaaccc
    12421 tcccggaggt caggacaaac aggctgcccg gccgtccacc ctcccgtatg atgtcctccc
    12481 cgacctcgaa cgtcgccgct tcggcctcct ccatgaactc ggcccgctcc tcgtcggtga
    12541 ggaactcgaa cagcctcacc gtccgtaact gattctccgt cacagggtca tcccctcatc
    12601 acagctatca ttgatgaaga ttatatgctt gccgctctcc acacgaaatg caacccgcgc
    12661 ggatcgttca gagccattcc cggtatcgtc catgatcctg gcgagcacag acatggtgcg
    12721 ccagaaaggg aaacggacgc ggtggaacta ccaccacgcc cgctcctgcg gcttctatga
    12781 tctttgaagc ctgctgaagc ccgctactcg atcacctgca tgtccacgac accggaaccg
    12841 gcggccgtca ggccgatctg ctcggcggcg gcttgcgaca ggtcaaggtc gcgtcccgcc
    12901 acgtaaggcc cccggtcgtt gaccgtgacg acaacggagt tgccgccgta ggtcaccagc
    12961 aactgcgtgc ccagcggcag ggtcttgtga gccgcggtca gcgcggtcgg gtcgaacggc
    13021 tgcccgctcg ccgtcagact gcccgccagc tcatagccgt agtagctcgc ctctaccggc
    13081 tcggcctggg cgtcttcctg cacaacagcc agcgaaacca cggagacacc cgcgaacaca
    13141 ccagccacaa agagcatctt gagaaaagac ttcaatagtc cccctccaga tcgcttgcat
    13201 aacagcgcag cagactacta cacagaattc gggttacaag aagcagcccg gctcccgccg
    13261 gtgacccgtt tcacatccgt tacgccgtat ggcattgagg cagatcgtgg caatatcgcg
    13321 ggtttcgccg caggtatggg agatggagcg cggccgtctc cggcaggccc gagggctcgg
    13381 gcgtcgagaa aagccggagt cattccatgc ttgtcgagcg gcggtaacgt tcggcgcggg
    13441 cctcgaactc ggggagcttg cggtcggcgg agcgctgggc gccgcggggc atcttgccag
    13501 agcgggcgag gtctatgtat gactcggtga cgcggagggc gtccgcatgg ttgcggcgac
    13561 gctcgtggag ccgagccagc gcctcgtagg ggtggctgcc gacgaagcgc tcggcgacgc
    13621 tcttctcgta gagggcgatg gcgcgctcgg ggtcccctgt ctgttcggcc ctggcggcct
    13681 cctcgtggcg ctcgaccgcc gcctcgtagc cgcgtagggg gtcttcttcc gtcccgatct
    13741 tcgccgtctc ctccggggcc aggctcgaaa gccccgatga tgccggagcc gtatcctctc
    13801 tgcgaccgcc gaagatcccg cgaagcctcc cgaacaccag cgcctccgtc ccgtatcacg
    13861 tccgtggcgt ggattatatt gcctgagagt cctgagaagg cgcggaccat gctttaatct
    13921 gcactcgttc gattgagagg agaacccatg ccccacgagt tcgcccggta cttgaaggtc
    13981 cgctcggcct acggcgccac ctggtcccgt gacggacgcc gcctcgcgtt cctgacggac
    14041 ataacgggcg tcccgcaagt ctgggaggtc ccggcggagg gcgggtggcc cgagcagctc
    14101 accttccacg aggagcgcgt catgggcgcc gagtattccc ccgccgggga ccgcatcgtc
    14161 ttcagcatgg acacgggcgg caacgagcgg gcccagatct tcctgctcga cccatccgcc
    14221 ggcgaggagt gcgacctgac caacgccccg gacgccatcc actacttcgg aggccactcg
    14281 cccgacggcg cgcgcatcgc ctacaccgcc acccgccgca acggaacgga cttcgacgtg
    14341 ttcgtccagg acctgccgga cggagagccg cagtgcgtct gggaagtatc cggctaccac
    14401 accgtcgccg actggggacc ggacggctcc ttcctcatag tctcgcgcca ccactccaac
    14461 ctgaacaacg acctctaccg gctagatctg gcgaacgcca gcggcgagcc taccctgctg
    14521 acgccccacg atggcgacgc ccgattccag tcggtaaacg tcacgccgga cggggcttcg
    14581 gcgtacctcg caaccgaccg cgacggggac ttcctgcgcc tggggcgcct ggacctctcg
    14641 acattggacc tcacttatct cacgccggac gactgggacg tggaagaggt gaagctctcc
    14701 agagacggcc gttacatggt ggcgagccgc aacgtggacg gttacacgga ggtccggttc
    14761 ttcaacggcc ggggcagccg caggcccggc ccgcaactgc cgaaaggcat actcggcggc
    14821 ttcgagttcg cgccggactc ggagcgtcta gccttcaccg tcaccgctcc ggagcgcaac
    14881 ccggacgtgt ggctcctgga cctgcccgaa ggcgagccgc ggcgtctcac ccgctcctcg
    14941 acggccggca tcccgccgcg caccttccgg cggcccaggc tcgtgcgtta cccgaccttc
    15001 gacgacggtc agatacccgc cctcttccac gaaccggatg ccgggagcgc tcccgtaata
    15061 gtaaacgtgc atggcggccc ggagagccag tcgcggcccg gcttcgcgcc cgtgacccag
    15121 tacctcatac accggggcta cgccgtcttc gcgccgaacg tccgcggctc caccggctac
    15181 ggcaaagagt acacgcacct ggacgacgtg gagctgcgga tggactcggt gaaggacttg
    15241 gcgcacgcgg cgcactggct gcgggagcag gggcacgaac gggtcgcggt catgggcggc
    15301 tcctacggcg ggttcatggt gctggcagcg ctcaccgagt acccggacct ctggagcgcc
    15361 ggggtggaca tcgtcgggat cgcaaacatg gtcaccttcc tggagaacac cggctcctac
    15421 cgccggcgct tgcgcgaacc cgagtacggg tcgctggaga aaaaccgcga gttcctggag
    15481 tcgatcagcc cgatccacaa ggccgaaaag ataaccgcgc cgctcatggt ggtccacggc
    15541 aagaacgacc cgcgcgtccc cgtcggagag gccgagcaga tcgtggagcg cgtaaagaac
    15601 aacggcggcg ccgtcgagta cctcctctac gaggatgagg gccacggcct agccaagcta
    15661 aagaaccgcc tcgacgccta cccccggata gccgccttcc tcgaccgtca cctcggtcca
    15721 tcggcatgac accggttttt gacacgtata tcacaagacg atagactggc gcagagcggg
    15781 gtgaaatagt ttccaatcag gaagggccga atatgtctgc gttgggtcgc cgatggggtc
    15841 ggggtgtcgt gttcgggctt ttgttggtcc tggcgacagt gatacctgcg gcggcgcagg
    15901 tggcgcctgg tgatggaaag tcgccgacgc tctcgacgac ggaccggctg gacgaccggc
    15961 ggtacgtgac gaccggggac cgagcctaca tagtcggtac ggaggcagga cggttcccgg
    16021 cgatggggtt ccacaccagg ggcgagatgg gcggcgtctg gagcccaccg atcaagctcc
    16081 tcgacggcat ctggttcggg atcgacgacc aatggatagg tccggccacc aaattcacca
    16141 gcggctacgg gcacgtcaac atgaaccttc ccgggcgcac cggcctccgg atcgggcgca
    16201 cggacttcgt tcccgacggg aagcgagccg tcctcgtcgg cctcaagttc agcgccggag
    16261 atacgcggca gagcttcacc ctcaagatgg acgcccactc agagttgatg ggcgcgtacc
    16321 cctggggcga gacgacgcca aatcagttga cgttcaatct ggcggacggc gtggcagtcg
    16381 aggacgggaa gctcgtgttc cgcgagcagg gcacgccgcc ggtagcgaac gccgaggccc
    16441 acgactgggc tgccgtagtg ggatccagcc ttacccccac cggctcggac gcgggcgcgg
    16501 acttccgcgg tccgcaggac ccggcggtca tctgcccggc ctccggcccg accgcgccgc
    16561 cggctccgcc gcgctgcgac gacacggagt acggcaaggg gaagggcggc gagctacgct
    16621 acaacgtcag cgtcccggcg aactccagca ggaccgtgtg gttcgcggtg tcgggctcgg
    16681 agtccggggc agcggcggcg aaggccacaa acgcttccgt gctgggcgat ccggaaggcg
    16741 cgctggataa gaaggtcacc ggacggctcg cgctgcagga gaagtcgaag ctcagtctgc
    16801 cgggcgatcg gcggttgcag aggagcatag actggagcaa gcaaaacctc gccgattcgg
    16861 tgcagagcgc cagggacctg gagatccggg agacaaacgc cggcaagaat tacccgccgc
    16921 cggaggggac cctggatagt atgcgcttcc tcggcgcggg cttcccggac tacccttggc
    16981 tcttcgggac ggacggcgag ttcaccgcgt tcgccagcgt cggggtcggg cagttcggcc
    17041 cgataaagga ccatatgcgg gcgctcaaga gggtcagcga gatagacaac ggcaactccg
    17101 gcaaggtcgt ccacgaggtc gtaacggacg gcagcgtgtt cttcgggtcg aacgcggatg
    17161 ccggcaacac ggacgagacg gccaagttcc cgtcggcggt cgccctgatc tggcgctgga
    17221 ccggcgacga cgccttccgt gacgagatgt atccgttcac ggtgaagaac atgcgctaca
    17281 tcttccgcgt gctggacaag gataacgacg gctggcccga gggactcggc aacgtcgagc
    17341 gcgagggcat gggcgaggag aagctcgaca acaccgtcta cactatccgc ggcctctacg
    17401 acctggcgga catggcgagg agcaagggag acaaggcgac ggccaactgg gccgaggaca
    17461 aggccgccac tatgagggcc aggttcgaga aggcgtggtg gttcgggcgc gagggggccg
    17521 cgcagtacgc cgactcgctc gacgatcccg gtaaccggca ggtcttccag cggcactgga
    17581 tcggggtgac gccgatggac gtggacctgg tcgacaagag cgaccgcacc gttccgggtc
    17641 tcgcctcccg gccgcacggc caggccgcgc tcgccgagcg ggagctagcc tgctacacgg
    17701 accgtttcgg catgtaccac accggcaccg gcccgacgag cgcggcgggc ggcaacaagg
    17761 gagccacctg cgacacccac gtctccgaag tccagagcga acgcaccatc ttcaccctga
    17821 acaccgcgat catggccgtc ggcgaaggca actacggccg cctcggaacc gaccagcaga
    17881 aacactacac gaaggccaac gccgaccttc agctcatccc ggacgagcag cccggcgcga
    17941 tgccggagat agccccctcg ccggactacg ggcgatccat agaccggccg ttcaacgagc
    18001 gggcgatggt gttacaggca tggggcgcgt acggcacgat ctggccggtc ctccaccagc
    18061 agctcggcat ccgacccgac atgggccggg gcgagctcgc ggtcgtgccg caggtcccgc
    18121 cgggctctcc cggactgtcg ggcgagaata tccggctcgt caagggctcg gtcgccgtct
    18181 cggcggatca cgaaggcaag acctacacca cgaaagtatc taccaggctc aaggtggacc
    18241 tgactatcgg gcacaccgtt ccgcgcgacg cgaaggtgag ttccgtcagg ctcaacggtg
    18301 acaaagtccg ctacaaggtc cgcaccacca accggggcaa ggaagtgctc gtgaaagctc
    18361 cgaagaccgg gaagcaggag ctggtcatca gagccggcta gcggagccag atccacgatt
    18421 ctccgggagg agcggtgcgc cgcccctccc gatccatgca agcagaattt acctggcgtt
    18481 aaccggcgtg taacaaaagc atacaagttt attgatatac ctgtgccctg gcggatccag
    18541 ctagaaggag gcaacacgag tgagcatcag cggcaactgg gacaggcgcg tcagcaggcg
    18601 agcgtttctt ggcatgggag gcatgagcgc ggcagctctc gtactgggtt ccagggaagc
    18661 gatggcgaaa aaggggagca cggcggggtt cggggaactc gttgcggacc ccggcggtct
    18721 tatagacctg cccaaagggt tccagtaccg cgtgatctcc gcgaagggct cccctctctc
    18781 cagcggcggc accgtacccg gagaccctga cggcatggcc gctttcgcgg ggccgaagaa
    18841 aggcacggcg ctcctggtcc gcaaccacga gttgaagccg gaggacggct cgcccgtcgt
    18901 cggcaggaac ccctacgacc cttcccagac cggcggcacg accggcatcc tggtgaacct
    18961 ggaggaccgt acggagataa aggacttcgt cacctcctcc ggcacgcgca acaactgcgc
    19021 gggcggcgcg acgccctggg ggacctggct cacctgcgag gaggaccgga ccacgggcca
    19081 cggctacgtc ttcgaggtag acccccggga tcccgagaac gacctctcga agaccccgat
    19141 ccgggcgatg ggtttcttct cgcacgaggc ggtggacata gatcccgcca ccggcatcgc
    19201 ctacctcacc gaggacgact tccgcggcgc cgtcacgccc gaccccaatc aggaagtcgt
    19261 ggcggactac tcgacctccg agatagaccc ctcgaccggg cagcgcggca ccggcacccg
    19321 cgtcagcttc ctctaccgct acatcccgga cgaccggagc cagaagcccg gcgcgctcca
    19381 gatgggcggc aggctgcagg ccctggcgct cgaagagaag cccaactaca acgtggacct
    19441 cgccagcccc ggccagaagt tcggcgtcgt ctggaaggac gtgaaccccg aggagccgca
    19501 cgagagcgcc gaggacgccg gcgcggcccg cttcgggcgg ctcgaaggcg cgtacttcgc
    19561 cggtggagcg ttctggttcg acgacaccat cggcggcgag aagcggctgg gacaggtctt
    19621 ccgctacctt cccgcgaccg agacgctgga gctcttctac gaagggaccg agccaggcaa
    19681 gatggagagc ccggacaaca tcgtcgtcac gccgtggggc gacctgtggt tcgccgagga
    19741 cgaggccgtg gacggcgact ccgtgaaccg cgtaatgggc ataacgcccg aaggacaggt
    19801 ctacgtcttc gcccgcaacg ccacgagcga ctccgagttc gccggtccca ccttctcccc
    19861 cgacggcaag accttcttcg tcaacatgca gggcgatggc ctgaccttcg ccatctgggg
    19921 cccgttcggc cggcacaact ccggccgcca gcgtcagatg gccgtcgccg cgccgccgaa
    19981 gtcgatggcg ccgaaagtct ccggcgaact cgccgaggca gccgataagt tcgggatgag
    20041 ccacctcgaa gccgccgcct acgagcggct cggagtttcg ctggcataac cggacgatag
    20101 atccttcgat ggatggggat cgcatccgcg gtccccgttc tcgtttgcgc tccatgtaga
    20161 gtcgccacga gatttaaagc ggggttcacg agcgtgaaac acaagtaacg atagatctgc
    20221 taccttatcc gaggcggcga tggacccgct gtctccgaga gaccgtagag acaaccaggg
    20281 aggaaacagg tggcgttaga tagattacgt gtggcgagcc ttctcttatc gtttgcagtg
    20341 gccttgatgc ttgcggcgac catgatctgg accgccggcg cccagtccaa gccgaacccc
    20401 aagcccgtgc ccgaggaccg catagccgac gtacagctac tcggcgtcaa cgacttccac
    20461 ggcaacctgg aacctccccg caccctgaac ggccgcgccg ttggcggcgc ggcctacctg
    20521 gacgcctaca tggacaagta cgcggcggcg aatcccgagc gcaccatccg ggtacacgcc
    20581 ggggacatgg tcggcgcctc gcccctcatc tccagctact tccacgacga accgaccatc
    20641 tacgccgcta accagatggg cttcgatgtc ggcactgtcg gaaaccacga gttcgacgag
    20701 ggcggcgagg agatgctgcg cctcataaac ggcgggcaac gcgacgacgg caagcagttc
    20761 aaggacggcg tggacggaga gcccgtcaac acctcggacc ccgacttccc aggagcggac
    20821 ttcccctaca tagcggcgaa caccgtctac aaggacactg gcaagagtgt gctgcccccg
    20881 tatcaggtcg tcaagcggga cggggtgaag gtcggcttca tcggggtgac gacgcttgag
    20941 acgccgaaca tcgtcgtccc ggacgcggtg gcgccgttcg attatctgga catctcggat
    21001 acggtcaaca agtacgcgaa gcagttgcag gacaagggag tccagaccat cgtggtgctg
    21061 gcgcactccg gagggaacca ggtctcggcg accgaggcta cgggtgagat cctggacgag
    21121 acctcccaga tgtcgaacgc cgttgacgtc gtcgtcgccg ggcactcgca ctcgctgctc
    21181 aacaaccggg tggggaacaa gctcgtcgtc gaggcgttca gcttcggcac cgccttcgac
    21241 gctgtggaca tgaagatcga tcgcaagacc ggggacgtaa cgagttcctc cgccgacatc
    21301 gtgaccacct acgatgacgc gatacagccc gacgcgaaga ccgccgagct ggtagccgac
    21361 tacaaggccc gcatcgcccc gatctccgaa cgggtggtcg ggaccgcgca gaccgacatc
    21421 tcgcgcacct caaacgtcgc cggtgagagc gcgatgggcg acctcatcgc cgacgcgcag
    21481 cgggactttg ccggcgccga cttcgcgttc atgaaccctg gcggcatccg ggcggacatc
    21541 gcggccggcg aggtcacgta tggcgagctg ttcaccgtcc aacccttcga caaccaggtc
    21601 gtctcgatgg atcttaccgg ggatcagatc taccgcctcc tcgaacagca gttcccgccg
    21661 aaccaggtgg ccggcacgaa gcgcatcctc caggtgagcg gactgaagta cacctacgac
    21721 gagtcgctcc ccgagggcca gcgcattacc tccctcacga agaccgacgg aacccccata
    21781 gaccgcgcaa cgacctacaa ggtggcggcc aacagcttta tcgcgaccgg cggcgaccgc
    21841 ttcacggtcc tgaaggaagg ccagaacgtc cagaccatcg gcggcgacct ggacgcgctg
    21901 gaagcctaca tagacagcct ccagcagccc ttcagcgcgc cggacccggc gggcgagcag
    21961 aggattacga aggccggata gagcgagcca ctaacacctc aacccatcgt cctcgcgggc
    22021 gcgccacgag gcgcgcccgt ttcgtcttgc cgaatataca cccggcgtct cgaaaagctg
    22081 taagctgact gcctaaagcg ccgaacggcc agcgagccaa gagagcagga ggttccagtg
    22141 gagactacga agttcttact cgacgagaag cagatccccg agtcctggta caacctcgtc
    22201 ccggacctgc cgttcgagtt ggagccgccg ctgaacccgg cgaccaggga gccggtggga
    22261 ccggaggcgt tcgcgccgat cttcccgcag gagatcatcc gccaggaagt aactaccgac
    22321 tcctacgtcg agatcccgga ggaggtgcgg gagatctaca gcctctggcg tccgaccccg
    22381 ctcttccgcg cccggcgcct ggagaagttc ctcgataccc ccgcccatat ctactacaag
    22441 tacgagggcg ttagtccctc cggctcccac aaaccgaaca ccgccgtccc gcaggcctac
    22501 tacaaccgcg aggagggagt ccggcggctc accaccgaga ccggcgccgg gcagtggggc
    22561 tcctcgctcg ccttcgcctg ccgtcagatg gggctggagt gtaccgtcta catggtccgc
    22621 gtcagctacg accagaagcc ctaccgccgc atcatgatgg agacctacgg agccgaggtc
    22681 catgccagcc ccacagacct caccaacgcc ggccgcaaca tcctcgagga gcatcccgac
    22741 tcgccgggct ctctggggat tgccatatcc gaggcggtcg aggacgcggt gatgcacgac
    22801 gacgccaagt actcactcgg cagcgtcctc aaccatgttc tgctgcacca gaccgtgatc
    22861 ggccaggagg cgctgcaaca gatggacctc gccggagagt acccggatat agtggtgggt
    22921 tgcgtcggcg gtggatccaa cttcggcggc gtcgcgttcc cgtttatccg ggagaacctg
    22981 aagaacgggc gagatacgcg catcgtggcc gtcgagcccg cctcatgccc gacgctcacg
    23041 aagggacgat acacctacga cttcggcgac acggccggga cgacgccgat gatgaagatg
    23101 tacacgctcg gccactcgtt cgtgccgagc ggcatccacg ccgggggtct gcgctaccac
    23161 ggcgactctc caatagtctc ggcgctcgtt cacgaggggc tcgtcgaagc gcgggcctac
    23221 gcgcagaacc cgaccttcga ggccggtgtg acgttcgccc ggacggaggg gatcgtcccg
    23281 gccccggagg tgacgcacgc gatcaaggcg accatagacc tggcgct
//
LOCUS       DASSMB010000132         7723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1044147, whole genome shotgun sequence.
ACCESSION   DASSMB010000132 DASSMB010000000
VERSION     DASSMB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044147"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VFJ72_08090"
     CDS             <1..453
                     /locus_tag="VFJ72_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7479457.1"
                     /translation="GFLGAGAIIRQGIDVRGLTTAASLWAAAAIGMASGAGYYFGAAA
                     TTVIVVATLYLLRELRSRVISRFRTEFGVLNVTFETVRGEVRGVLEALDRHGMTVRNM
                     ETEIESGQARYSLQLRVPPDGNVQEMLGEVSELPGVRHVGMTGLHEAE"
     gene            complement(476..2566)
                     /gene="ppk1"
                     /locus_tag="VFJ72_08095"
     CDS             complement(476..2566)
                     /gene="ppk1"
                     /locus_tag="VFJ72_08095"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HET7479458.1"
                     /translation="MTDVARKPNLSDPALYINRELSWLEFNDRVLEQARDERHPLLER
                     ARFISISETNLDEFFMIRVAGLQQQVASQLPNPVPDGMTPEEQISRIKENTEEFFDER
                     RRILDGEILPGLQKEGIRLVPHGKVPVKGRRELRKTFARDILPILTPLAIDPAHPFPH
                     ISNLSLNLLVVIDDGGRKVMARVKVPNTIPRFMRIPPTEKLEETPQPGKHGEIRLTRL
                     EEVIAANLDELFPGKKILANYVFQVTRNADFVIEEDEASDLLQAIEDEIEGRWFGQSV
                     RLVVTDEMPNDLRDWLARNLRLTETDVYAVPEPIGLADFEELTHLDRSDLLYPPISPR
                     IPPEIRSARSITQAIRSGDILLYHPYDSFTPVIDFVRAAANDPEVLAIKQTLYRVGSN
                     SPIVEALSEARDEQTQVAVLVELKARFDEEPNITWARQLEARGVHVAYGIVGLKTHAK
                     ICLVVRREGSGLRRYIHLGTGNYNPGTARIYTDFSYFTDDPNLAEDGSDLFNYLTGYS
                     EHEEYHELLVAPTTLRDNIARLIEEQTEKARNGEPARITCKTNSLTDPQLIELLYEAS
                     QAGVKIALIVRGICCLKPGLEGISENIRVVSIVGRFLEHARILAFGEGKDEKIYLGSA
                     DLMQRNLDRRVETTFPLREEHHRQKVRRLLDLQLSDNMNAWELKPDGTYERLSPKDEE
                     EPLDSQAVLLEEPF"
     gene            complement(2563..3450)
                     /locus_tag="VFJ72_08100"
     CDS             complement(2563..3450)
                     /locus_tag="VFJ72_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479459.1"
                     /translation="MSRYGVIDVGSNTIHLLVGEVQDGGVLPVTGEKVSARLGAGVEK
                     TGKLDDERLALAAAAIGLFARISALNDVPEPVILATSAVRDAENGPELNERVVDSTGL
                     KMQLISGEKEAELGFRGAVSAVGKMWEGPALVVDLGGGSAQLIVGEPSSGPLMQVSLP
                     LGSNRTTERFVSKDPAKKKELRALDEHVKEIMPGWSLASQVPVVAVGGSARAMLKITS
                     DKLTVERLRSLAEEVSEEPSAVLAREYGLAPERAKVMPAAITTLAAILEHFGRDELTV
                     ARGGIREGTLITLAEGKEI"
     gene            3527..3991
                     /locus_tag="VFJ72_08105"
     CDS             3527..3991
                     /locus_tag="VFJ72_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET7479460.1"
                     /translation="MVYSILQGQAPRRLASQDHGSEPGCVISKRKNSKKTKTLDGRVP
                     AAARAKVRDYGSHVLICAGSDCKKRGAKEVRRAFKDSLRADGINREVRTDTVDCLGLC
                     KHGPNAVVYPSGTWYLGLTEYDIPEIVESHLVNGEPVERLSAERRPVKKRKK"
     gene            complement(4035..4643)
                     /locus_tag="VFJ72_08110"
     CDS             complement(4035..4643)
                     /locus_tag="VFJ72_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET7479461.1"
                     /translation="MKKYRKQRSKTLVTALSALAVLAGLGLLGYSLFGGTAASAPEIG
                     TVKTGLIGAPEKPVAQRDTNMTLTVPKMARVNDVPVYTAPASDEATLDRGTMHVEGTG
                     FPWETGSNVYIAGHRLGYRNTGSYLQFYDLPKLKKGDEIILTGADGTTYTYEVFRKFR
                     VDPSNYSVTQPVPGKSIVSLQTCTLPYYTQRLIVQGELVSVS"
     gene            4823..6151
                     /locus_tag="VFJ72_08115"
     CDS             4823..6151
                     /locus_tag="VFJ72_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7479462.1"
                     /translation="MKVGVPKEMAEGENRVGIVPETVEKLVKEGFEVLVESGAGRDYN
                     LDENYEEAGAEIVGGAEEVYSEADLIVKVARPTEDEIGHMHEGQSLVCFLNGPQYPEL
                     VKKLADAGVTVFSNEAIPRTSVAQSMDALSSMGSVAGYKCALIGADTLGKYVPMLSTA
                     AGTTKAANVLVLGVAVAGLQAIAIMHRLGANVFAYDIRPETKEQAQSLGASFIDSDDE
                     KENEEEDEFVEYKPEGFNKFMASLGFYSFAEPPRDKYVVEGEEDDSEDEEEDEGWSKE
                     KLEEDQKLLRERMKEMDIVITTALVPGRKAPKLVDKEMVESLKPGSVIVDLAAESGGN
                     CELTEPGETVEHSQVKIVGPVNLPSAMPIHASQLLSRNMFNLIKHITEDKSEDKEEKQ
                     PELALDFEDEIMDKTCIAHDGEVRDESTREALEEAQSEAKTDGSGESEAS"
     gene            6152..6460
                     /locus_tag="VFJ72_08120"
     CDS             6152..6460
                     /locus_tag="VFJ72_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7479463.1"
                     /translation="MQTLLAQFAILVIAAFLGFELISRVPNLLHTPLMSATNAIHGVI
                     LVGGMIALAEATDPIIKVVGFIAVFFGAANVVGGFWVTNRMLGMFRPPVKKVKRRREA
                     "
     gene            6464..>7723
                     /locus_tag="VFJ72_08125"
     CDS             6464..>7723
                     /locus_tag="VFJ72_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7479464.1"
                     /translation="MQVATFLAYLAAAALFIVGIRRLRKPETARSGNTLAAVGMTIAL
                     IATLFVIQFQDLFLIGTAALLGAVVGAISAQRVQMTAMPQMVAAYNGVGGGAAALIAL
                     SEFYHGQTVGGESLVATITVPLTILVGSVSFMGSVVAFLKLQELAFSGSVTFPLQQIV
                     NALLFIGILVLAANAISDGAILPFISPIASIVVVLVASAILGVLLVIPIGGADMPIVI
                     SLLNAFTGLAAAASGFVLGNYILIIGGTIVGASGTILTRLMSSALGRPLGKIIFAGIA
                     AAGSSKKEDQEDREVNDVNAGAVGEYLSDEAQKVVIVPGYGLAVAQAQGAMRELMEAL
                     EKQGKEVLFGIHPVAGRMPGHMNVLLTEAGVPYDKLYDLEDINPEFADTDAVIIVGAN
                     DVVNPAANDPDQNTPISGMPVLDVEQAE"
BASE COUNT         1385 a         2496 c         2457 g         1385 t
ORIGIN      
        1 ggtttcctgg gtgcgggcgc gatcatccgc caggggatag acgttcgggg gctcaccacg
       61 gcggcgagcc tctgggccgc cgcagcgata ggcatggctt ccggcgccgg gtactatttc
      121 ggcgccgcgg ccacaaccgt catcgtggta gccacgctct acctcctgcg cgagttgcgg
      181 agccgcgtga tctcgcgctt ccgcacagag ttcggtgtgc tgaacgtaac cttcgagacc
      241 gtgcggggcg aggtgcgggg cgttctggag gcgctcgacc gacacggcat gacagtccgt
      301 aatatggaaa cggagataga gtccggacaa gcccgctaca gcctgcaact gcgcgtcccg
      361 ccggatggga acgttcagga gatgctcggc gaggtgtccg agctccccgg cgtccgacac
      421 gttggcatga ccggcttgca cgaagccgag taaggtcgag taaggccaga ggagtttaga
      481 acggctcttc cagcaggaca gcctggctgt cgagcggctc ctcttcatct ttggggctca
      541 ggcgctcgta tgtgccgtcc ggcttcagct cccaggcgtt catgttgtcc gagagctgga
      601 ggtccaggag gcggcggacc ttctggcggt ggtgctcctc gcgcaggggg aaggtggtct
      661 cgacgcggcg gtcgaggttc cgctgcatca ggtcggcgct gccgaggtag atcttctcgt
      721 ccttcccttc tccaaaggcg aggatgcgcg cgtgttccag gaagcgtccg acgatggaga
      781 cgacgcggat gttctcgctg atcccttcca ggcccggctt cagacagcag atgccgcgca
      841 cgatcagggc tatcttcacg cccgcctgcg aggcttcgta gagaagctcg atcaactgcg
      901 ggtccgtgag cgagttggtc ttgcacgtta tccgcgcggg ctccccgttc cgggccttct
      961 ccgtctgctc ctctatcagc cgcgcgatgt tgtcgcgcag cgtggtcgga gctacgagca
     1021 actcgtggta ctcctcgtgc tcggagtacc cggtcaggta gttgaagagg tccgagccgt
     1081 cctcggccaa attcgggtcg tccgtgaagt atgagaagtc cgtgtagata cgagccgtgc
     1141 cggggttgta gttgccggtg ccgaggtgga tgtagcgccg gagccccgac ccctcccgcc
     1201 ggacgaccaa gcatatcttc gcatgcgtct tcaggcccac gatgccgtag gcgacgtgta
     1261 cgccccgcgc ctctagctgc cgcgcccagg tgatgttcgg ctcctcgtcg aaacgcgcct
     1321 tcagctccac cagcacggcg acctgcgtct gctcgtcgcg ggcctcggag agcgcctcga
     1381 ctatcggaga gttcgagccg acgcggtaga gggtctgctt tatagcgagg acttcgggat
     1441 cgttggcggc ggcccggacg aagtctatga ccggggtgaa cgagtcgtag ggatggtaga
     1501 gcaggatgtc gccgctacgg atcgcctggg tgatggagcg ggcgctccgg atctccggcg
     1561 gtatccgcgg cgagatcggc gggtagagga ggtccgagcg gtccagatgc gtaagttcct
     1621 cgaagtccgc gagccctatc ggctccggca cggcgtacac gtcggtctcg gtgagccgga
     1681 ggttgcgggc cagccagtcg cgcaggtcat tcggcatctc gtccgtgacg acgagccgca
     1741 cgctctggcc gaaccagcgg ccctcgatct catcctctat cgcctgcagg aggtccgagg
     1801 cttcgtcctc ctctataacg aagtccgcat tgcgcgtaac ctggaagacg tagttcgcca
     1861 ggatcttctt tccggggaac aactcgtcca ggttggcggc gataacctcc tccagccgcg
     1921 tcagccttat ctccccgtgc ttgcccggct gcggcgtctc ctctagcttc tcagtcggcg
     1981 ggatccgcat gaagcgcggg atcgtattcg gcaccttcac gcgggccatg accttgcggc
     2041 cgccgtcatc tattacgacg agcaggttca gcgagaggtt ggagatgtgc ggaaaggggt
     2101 gcgccgggtc tatggcgagc ggcgtcagga tcgggagtat gtcgcgcgcg aaggtcttgc
     2161 gtagctcgcg ccggcctttg acggggacct tgccgtgcgg gacgagccgt atgccctcct
     2221 tctggagccc cggcaggatc tccccgtcca gtatccggcg ccgctcgtcg aagaactcct
     2281 cggtgttctc cttgatgcgg gagatctgtt cctcgggagt catcccatca gggaccgggt
     2341 tagggagctg agaggcgacc tgctgctgaa gtcccgccac ccggatcatg aagaactcat
     2401 ccaggttcgt ctcggatatg gagatgaaac gcgcccgctc cagaagcggg tgccgctcgt
     2461 cccgcgcctg ctccagcacc cggtcattga actccagcca cgacagctcc cggtttatgt
     2521 acagcgccgg gtccgaaagg ttcggcttgc gcgctacgtc ggtcatatct ccttgccctc
     2581 cgccagcgtg atcaacgtcc cctcccttat ccctccgcga gcgacggtca actcgtccct
     2641 cccgaagtgc tccaggatcg ccgccagcgt cgtgatcgcc gccggcatta ccttcgcccg
     2701 ctccggcgcc agcccgtact cccgcgccag caccgccgag ggctcctccg agacctcctc
     2761 cgcgagactc cgcaagcgct ccaccgtcag cttatcgctc gttatcttca acatcgcccg
     2821 cgccgaaccg ccgaccgcca cgaccggcac ctgcgacgcc aggctccatc ccggcattat
     2881 ctccttgacg tgctcgtcga gcgcccgaag ctctttcttc ttcgccgggt ccttgctcac
     2941 gaagcgctcc gtggtgcggt tggaaccgag cggcagagag acctgcatca gcggtccgct
     3001 cgacggctcg ccgacgatga gctgggcgga cccgccgccg aggtccacca cgagcgccgg
     3061 cccctcccac atcttcccga ccgccgagac cgcgccccgg aagcccagtt ccgcctcctt
     3121 ctcgccggag ataagctgca tcttcaggcc cgtcgagtcg accaccctct cgttcagttc
     3181 cgggccgttc tcggcgtccc gcacggcgct cgttgcaagt atcacgggct cggggacatc
     3241 gtttagcgcg gagatcctgg cgaacagccc tatcgccgcc gccgcgagcg cgagccgctc
     3301 gtcgtcgagc ttgccggtct tctccactcc cgctccgagc cgagccgata ccttctcccc
     3361 ggtaacgggc agcactcccc cgtcctgcac ctctccgacc agcaggtgga tcgtgttgga
     3421 cccgacatct ataaccccgt accggctcaa gccctcatcc ctcccgattg ccgccaaacc
     3481 cggcgtgaac tctcacaccg accatgatac cgcccacgca aggctgatgg tatattcgat
     3541 tcttcagggc caggctccgc gcagactcgc ctcccaagat cacggctcgg aaccggggtg
     3601 cgttatctcg aagagaaaga actcgaagaa gacgaaaacg ctggatggga gagtccccgc
     3661 cgctgcgaga gcgaaggtcc gggactacgg ctcgcacgtc ctgatctgcg ccgggagcga
     3721 ctgcaagaag cgtggcgcga aggaggtccg ccgcgccttc aaggacagtc tgcgcgccga
     3781 tggcattaac cgcgaggtcc gcactgacac ggtggactgc ctgggcctct gcaagcacgg
     3841 ccccaacgcc gtcgtctacc cctccgggac gtggtacctg gggctcacgg aatacgacat
     3901 accggagatc gtcgagagcc acctcgtaaa cggcgagccc gtggagcgcc tctccgccga
     3961 acgccgcccg gtcaagaagc gcaagaaata agagcccgca gcggggtttc cgctgcgggc
     4021 tctcgatcct gtcactagct cacgctcacc agctcgccct ggacgatcag gcgctgcgta
     4081 tagtagggca gggtgcaggt ctggaggctg acgatgctct tgccgggcac gggctgagta
     4141 acggagtagt tactcgggtc gacgcggaac ttcctgaaga cctcataggt gtacgtcgta
     4201 ccatcggcgc cggtcaggat gatctcatcg cccttcttga gcttcgggag gtcgtagaac
     4261 tgcagatagc tgcccgtgtt cctgtaaccg agccggtgtc ccgcgatgta cacgtttgag
     4321 ccggtctccc acgggaatcc ggtcccctcg acgtgcatgg tccctcggtc cagggtggct
     4381 tcgtcgctcg cgggggcggt gtagaccggc acgtcgttca cccgggccat cttcgggacg
     4441 gtgagcgtca tgttcgtatc cctctgcgcc accggcttct cgggagcgcc gatcagaccc
     4501 gtcttgaccg tcccgatctc gggggccgat gccgccgttc ccccaaagag cgagtaaccc
     4561 agcaggccga gcccggccag aaccgccagc gcgctcaacg ccgtcaccag agtcttcgac
     4621 cgctgcttcc gatacttctt catcgtgctc gacccccttt cgagaagttg ctcttatcct
     4681 tcactgataa agataattcc cgcaggtgac atatcggtta caaaaaggat gtttctggat
     4741 aacacttttg gtccggggca cgcccgagcg agtgctggag cagctttaca cacatccttt
     4801 ttttctgtat ataatgcgcg gcgtgaaggt aggggttccc aaagaaatgg ccgagggcga
     4861 gaaccgggtc ggcatcgtgc cggagaccgt ggagaagctc gtcaaggagg gcttcgaggt
     4921 gctcgtggag tccggcgcgg gccgcgacta caacctggac gaaaattacg aagaggccgg
     4981 agcggagatc gtcggtggcg ccgaagaggt ctacagtgag gcggatctga tcgtcaaggt
     5041 cgcccgtccc accgaggatg agatcgggca catgcacgag ggccaatcgc tagtctgctt
     5101 cctgaatggc cctcagtacc cggaactggt caagaagctc gccgacgccg gcgtaactgt
     5161 cttctcgaac gaggccatcc cccgcacgag cgtcgcgcag agcatggacg ctctctcctc
     5221 gatgggctcc gtcgccggat acaagtgcgc gctgatcggc gcggataccc tgggcaagta
     5281 cgtaccgatg ctctccaccg ccgccggcac cacgaaggcg gcgaacgtcc tggtcctggg
     5341 cgtggcggtc gccgggcttc aggccatagc catcatgcac cggctcggtg cgaacgtctt
     5401 cgcctacgac atccggcccg agaccaagga gcaggcccag tccctgggcg catccttcat
     5461 agactcggac gacgagaagg agaacgaaga ggaagacgag ttcgtggaat acaagcccga
     5521 gggcttcaac aagttcatgg cctcgctggg cttctactcc ttcgccgagc cgccgagaga
     5581 taagtacgtc gtggaaggcg aggaagacga ctccgaggac gaagaggaag acgaggggtg
     5641 gtcgaaggag aagctggagg aggaccagaa gctcctgcgc gagcgcatga aggagatgga
     5701 cattgtcatc acgacggcgc tcgtgcccgg caggaaggcc ccgaagctcg tggacaagga
     5761 gatggtcgag agcctgaagc cggggagcgt gatcgttgac ctcgcggccg agagcggcgg
     5821 caactgcgag ctaacggagc caggcgagac ggtcgagcac agccaggtca agatcgtggg
     5881 tccggtcaac ctgccgagcg cgatgccgat ccacgcgagc cagctactct cgcgcaacat
     5941 gttcaacctc ataaagcaca tcacggaaga caagtccgag gacaaggaag agaagcagcc
     6001 cgagctggcg ttggacttcg aggatgagat catggacaag acctgcatag cccacgacgg
     6061 cgaggtgcgc gacgagtcca cccgcgaggc gctggaggaa gctcagagcg aggcaaagac
     6121 cgatggttcc ggcgagagcg aggccagcta gatgcagaca ctgctcgccc agttcgcgat
     6181 cctggtcatc gcggccttcc tgggattcga gttgatctct cgggtcccga acctgctgca
     6241 cacgccgctg atgagcgcga ctaacgcgat ccacggcgtc atactggtgg gcggcatgat
     6301 cgcgctggcc gaggccacgg atcccataat caaggtggtg ggctttatcg cggtgttctt
     6361 cggggcggcg aacgtcgtcg gcggcttctg ggtgacgaac cggatgctcg ggatgttccg
     6421 cccgccggtg aaaaaggtca agagaagaag agaggcataa cccttgcagg tcgctacgtt
     6481 cctggcctac ctcgcggccg cagccctgtt catcgtcggc atccgccgcc tgcgcaagcc
     6541 cgagacggca cgctcgggca acaccctggc agccgtgggc atgaccatag ccctgatcgc
     6601 gacgctcttc gtcatccagt tccaggacct cttcctgatc gggacggcgg ccctcctggg
     6661 cgcggtcgtc ggggcgatct cggcgcagcg ggtgcagatg acggcgatgc cccagatggt
     6721 cgcggcgtac aacggcgtcg gcggcggggc ggcggcgctc atcgcgctct cggagttcta
     6781 ccacggacag accgtgggcg gggagagcct cgtggcgacg atcacggttc cgctcaccat
     6841 actcgtcggg tcggtcagct tcatggggag cgtggtggcg ttcctgaagc tccaggagtt
     6901 ggccttctcc ggctccgtca ctttcccgct gcaacagatc gtaaacgccc tgctctttat
     6961 cggcatcctg gtcctggcgg cgaacgccat atcagatggg gccatactcc cgttcatctc
     7021 gccgatagct tccatagtcg tcgtgctggt cgcctcggcc atcctgggcg tgctgctcgt
     7081 cataccgata ggcggggcgg acatgccaat cgtcatctca ctattgaatg cctttaccgg
     7141 gctcgcggcg gctgcctcgg ggttcgtgct ggggaactac atcttgatca tcggcggcac
     7201 catcgtcggc gcctccggta cgatcctcac gcgcctgatg tccagcgccc tggggcgtcc
     7261 gctcgggaag atcatcttcg ccggcatcgc cgccgcgggc agtagcaaga aagaggatca
     7321 agaagaccga gaggtcaacg acgtgaacgc cggagcggtc ggcgagtacc tctcagacga
     7381 ggcccagaag gtcgtcatcg tccccggata cggcctcgcc gtcgcccagg cccagggagc
     7441 catgcgggag ctgatggagg ccctcgaaaa acagggcaag gaggtcctct tcggcatcca
     7501 cccggtagcg ggccgcatgc ccggccacat gaacgtcctg ctgacagagg cgggggtccc
     7561 ctacgacaag ctctacgacc tcgaagacat aaaccccgag ttcgccgaca ccgacgccgt
     7621 aatcatcgtc ggcgccaacg acgtcgtgaa cccggcggcc aacgacccgg atcaaaacac
     7681 cccaataagc ggcatgcccg tcctggacgt ggaacaggcc gag
//
LOCUS       DASSMB010000133         5999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_409356, whole genome shotgun sequence.
ACCESSION   DASSMB010000133 DASSMB010000000
VERSION     DASSMB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409356"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFJ72_08130"
     CDS             <1..835
                     /locus_tag="VFJ72_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET7479465.1"
                     /translation="GDLLSRAILEGCAVYAAHTSYDAAPGGVSVALADALGLCGPMEV
                     VVPRGELRKLVIFVPAENVDDVADTLSKAGAGVIGDYTNCTFRLPGTGTFLGGEGSDP
                     YLGEKGRLESVEEIRLETIVPAHLAHDALAAAVAAHPYEEAAFDIYPVEGHPEGCGYG
                     RLGRLPEPLTPEELREHVSDSLGFPARLVAAPRVGSVRRVVVLGGSGGSFIREAAASG
                     AEAYVTGDLNYHDGLLAESLGLVAVDAGHAATELPALEPLARRLAALVDVPVEVSRVR
                     R"
     gene            complement(851..1801)
                     /locus_tag="VFJ72_08135"
     CDS             complement(851..1801)
                     /locus_tag="VFJ72_08135"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HET7479466.1"
                     /translation="MGRIVALGEVVADIYRDEETSPVEMPFTARPGGAPANVAVAAAR
                     LGAAAAFAGGVGNDLFGAFILRALESEGVDTSGVLRCEPPTRTSLAFVEIGADGDRSF
                     TFYRSDPASDELLSPEGITRDLLSGASFVNFGSIPLIKDPARAAIHRAAELAREMDVP
                     VAFDVNLREHLWPSLAAARETVDPLLDLSTVVKMGHDELEPMLGTSDMEEAATMLLDR
                     GAALVLVSLGAEGAFYAAREFSGTLPAFEVESVDPTGAGDAFLAGVLTHLADAEWTEE
                     WVREATRRGTAAGALACTSFGAMGPLPTKDELERFMASAG"
     gene            complement(1832..2896)
                     /locus_tag="VFJ72_08140"
     CDS             complement(1832..2896)
                     /locus_tag="VFJ72_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HET7479467.1"
                     /translation="MDAYPIKLSYHVRSYYFGERLIPNLLGKRGVPEGTVAETWEISD
                     YRETTGTVLNGEYAGRTLHDLTLEFPEDLVGEGWNGPHFPLLEKFLDASHMLPVHLHP
                     DDETARRVHNEPHGKTEAWHILWAAEDSTILAGVKEGLSREELHDAFKARDYGAVMPR
                     HRIQAGDTVYVPGGIIHTFGPDTLIFEVQQTSDLGQFVMPDDLYGHPLDEATWEANIQ
                     ATLDELKTDYLPRPNPGLVLDDGPNRRVLCAAGPHFALERWTLTQPYTESAHPRRCMT
                     LSNVGGAVEIESGGGKERLVRGESCILPASIGEVRIVPEGESSLVVCYVPDLDLDVVA
                     PLRKAGYADEEIRTLGEVVV"
     gene            3274..4662
                     /locus_tag="VFJ72_08145"
     CDS             3274..4662
                     /locus_tag="VFJ72_08145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HET7479468.1"
                     /translation="MAQAASSSGSGGNRRFVYVVASVAALGGLLFGYDTGIISGALLF
                     IKKDFQLNPFLEGFIVSSLLLGAVIGAGVSGALSDRLGRRTIILVAAIIFAIGAVGAG
                     LAPNVGTLILFRVLLGLGVGSASALVPSYISESAPTDVRGSLSTLFQLAITIGILVAY
                     LVNAAFATAGDWRWPVGLAVIPALVLFVGMYFLPDTPRWLVSKGREEEARRVLRRTRT
                     DEEVERELNDIRRIEEAEEEQAGYRELLAPWVRPMLVVGIGLAVFQQFVGINTVIYYA
                     PTIIKSTGLADVASVLATVGIGVVNVLMTVVAILVIDRVGRKPLLLIGLAGMVISLGI
                     IGGAFAFSGLSGIISWVTLIGLMLYIASFAVSFGPLLWVMLPEIFPLNARGSGTGLSA
                     LSNWVANFIVAQAFLPLVGLIGRSAVFWILAGICVIAALFVHFVVPETRGRSLEQIEA
                     DLRGDREAAATT"
     gene            4738..>5999
                     /gene="murJ"
                     /locus_tag="VFJ72_08150"
     CDS             4738..>5999
                     /gene="murJ"
                     /locus_tag="VFJ72_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HET7479469.1"
                     /translation="MSAILRSVLSMSAATGLSRLTGFLRTTVMAATLGGAVVANAYTV
                     ANVLPTQIYELFMGGILSSTFIPLLVDRLTRHGEEDARRLTNALVTVVLPFLAVVALL
                     GVVFAPQIVALTTDWGAAEKLSPEEARRTTELAVLLFRVMAVQIFFYGAGAISTGVLN
                     SHRRFFLPTFAPVLNNLIVISSLVGYALLARKDFTAAIYILAGGTTLGVAVMSLVLVP
                     AVLKLGYRPRPVLGHPALADAVKLALPMLVFVAASVGVQVAAYRFGTQFSAAAQLLYA
                     FVIFQLPYGIFVVSIGTALMPELSERYASEDTDGYRATLSFGLRTMAFIAVPASVGMV
                     ALAQPIVGLLYERGQFTPQDTATVATLLVAYGVGLLGYGAYFVLVRSFYSRQNTRTPA
                     TLNLGLLVLYIALAYTLSATVGLIGVALA"
BASE COUNT          893 a         2038 c         1952 g         1116 t
ORIGIN      
        1 aggggatctc ttgtcccgcg cgattcttga ggggtgcgcc gtatacgcgg cccacaccag
       61 ctacgatgcc gctccgggcg gcgtctccgt cgcgctggcg gatgctctag ggctgtgcgg
      121 gccgatggag gtcgtggtcc cgcgcgggga gctccgaaag ctcgtgatct tcgtcccggc
      181 cgagaacgtg gatgatgtgg cggacacact cagcaaggcg ggggccggcg tgatcgggga
      241 ctatacgaac tgcacttttc gtctgccggg tacaggcact tttctcggtg gcgagggctc
      301 ggacccgtac ctgggcgaga agggacggct ggagagcgtc gaggagatcc ggctggagac
      361 catcgttccg gcgcacctgg cacatgacgc gctggccgcg gccgtcgccg cccatcccta
      421 tgaggaggcc gcgttcgaca tataccccgt ggaaggccat ccagaaggct gcggttacgg
      481 tcgcctcggc cgtctgccag agccactcac accggaggag ttgcgcgagc acgtctcgga
      541 ctcgctgggg tttccggcac ggctcgtcgc ggctccgcgg gtcggaagcg tgcggcgggt
      601 tgtggtgctc ggcggctcgg gtggctcttt tatccgggag gcggcggctt cgggcgcgga
      661 ggcttatgtc acgggagacc tcaactacca cgacgggctg ctggccgagt cgctgggcct
      721 cgtggcggta gacgccggtc acgcggcgac ggagttaccg gcgctcgaac cgctggcgcg
      781 gcggctggcg gctctcgtag acgtgccggt cgaggtcagc cgggtgcggc ggtaggcgaa
      841 gctgcgggtc tcacccagcg ctcgccatga aacgttccag ttcgtctttc gtgggaagcg
      901 gccccatggc gccgaagctc gtgcaggcga gcgctccggc cgccgtaccc cgccgcgtcg
      961 cctcacgcac ccattcctcg gtccactccg cgtccgcgag gtgcgtcagg acgccggcga
     1021 ggaaggcgtc cccggcgccg gtcgggtcca cggactcgac ctcgaaggcc ggcagggtgc
     1081 cggagaactc gcgggctgcg tagaaggcgc cctccgcccc caggcttacc agaacgaggg
     1141 cggcgccccg gtccagtaac atcgtcgcgg cctcttccat gtccgaggtg ccaagcatgg
     1201 gttccagttc gtcgtggccc atctttacga cggtcgagag gtcgagcaat gggtccacgg
     1261 tctcgcgggc ggcggcgagg ctgggccaca ggtgctcgcg caggttcacg tcgaaggcga
     1321 ccgggacatc catctcgcgg gccagttctg cggcgcggtg gatcgcggcg cgggcggggt
     1381 ccttgatgag cggtatgctc ccgaagttca cgaacgacgc cccggagagc aggtcgcgtg
     1441 tgatgccctc cggcgagagc aactcatcgg aggccgggtc ggaacggtag aacgtgaagg
     1501 agcggtcgcc atccgcgccg atctccacga aggcgagcga ggtgcgcgtg gggggctcgc
     1561 agcgcagtac gccggaggtg tccaccccct cggactcgag cgcccgcagg atgaacgcgc
     1621 cgaagaggtc gtttccaacg ccaccggcga aagccgccgc agccccaagc ctcgccgccg
     1681 cgaccgccac gttcgccggc gctcctcccg gccgcgctgt aaacggcatc tcgacgggcg
     1741 aggtctcttc gtccctgtag atgtccgcga cgacctctcc gagagccact atccttccca
     1801 cgtcatcctc cccatctcta ccgcactctc tctagacaac gacttctccg agcgtccgta
     1861 tctcctcatc cgcgtacccg gcctttcgca gaggcgcgac cacatcgagg tccaggtccg
     1921 gcacgtagca gaccacgagg ctcgactctc cctccggcac gatccgcact tccccgatgg
     1981 acgccggcag aatacacgac tcgccgcgca cgagcctctc ctttccgccg ccggactcga
     2041 tctccaccgc gccgccgacg ttcgagaggg tcatacagcg ccgggggtgt gcggactcgg
     2101 tgtatggctg ggtcaacgtc cagcgttcga gagcgaagtg cggaccggcg gcgcagagga
     2161 cccggcggtt cggtccatca tccagcacca ggccggggtt cgggcgcggc aggtagtccg
     2221 tcttcaactc gtcgagggtg gcctggatat tcgcctccca ggtcgcctcg tccagggggt
     2281 gtccgtagag gtcgtccggc atcacgaact ggcccagatc ggaggtctgc tggacctcga
     2341 agatcagggt gtccggcccg aaggtgtgga tgatgccacc cggcacgtag accgtgtcgc
     2401 cagcctgtat ccggtggcga ggcatcacgg ctccgtagtc ccgcgccttg aaggcgtcgt
     2461 gcagttcctc gcggctcaga ccctccttca ccccggccag tatagtcgag tcctcggcgg
     2521 cccagaggat gtgccaggct tcggtcttgc cgtgcggctc gttgtggact cttctggcgg
     2581 tctcgtcgtc gggatggaga tgaacgggga gcatgtggga ggcgtcgagg aacttctcca
     2641 gcagcgggaa gtgcggcccg ttccagccct cccctacaag atcctcggga aactccagcg
     2701 tcaggtcgtg cagggtgcgt ccggcgtact cgccgttcag taccgtcccg gtggtctcgc
     2761 ggtagtcgct aatttcccag gtctcggcga ccgtgccttc gggcacgccg cgcttgccga
     2821 ggaggttcgg gatcaaccgc tccccaaagt agtaggagcg gacgtggtag gagagcttta
     2881 tcgggtacgc gtccacagaa ctcccctttc caaatacttc ctccaacctc cgacgagagt
     2941 attctaacct cgtcgcgttt ccccgcgcct ctcgtcagac tggagagatg tggccagcct
     3001 ccagagcttc ttcaggctca atggacgtgc tgggatcatg cccagccgtt ttgacggttt
     3061 tccatacaac atcgggtatg aatcgccggg cgcgggtagc gttcgctccc gctacaacgg
     3121 cggagctgag tcaaccctgg agagccgcga agatgagttc gccaggttag cgagcggcca
     3181 gagtgttgac agggagtacc aaaagaggca gagtccttaa ggtagcgggc tttcgggacc
     3241 tgttcaacgc gcgtacaggg agaggagaca agtatggctc aggcggcgtc ttcgagcggc
     3301 tcaggcggta accgtcgttt tgtgtatgtg gtggcctccg tcgccgcact gggagggttg
     3361 ctcttcggct acgacacggg gatcatctcc ggcgcccttc tgtttatcaa gaaggacttc
     3421 cagctcaacc cgttcctcga agggtttatc gtcagctcgc tgcttctggg tgcggtgatc
     3481 ggagccgggg tcagcggggc gctctccgac aggcttggcc gacgcacgat catcctggtg
     3541 gcggcaatta tcttcgccat tggagccgtc ggcgcgggcc tggcgccgaa cgtgggaacg
     3601 ttaatcttat tccgcgtctt gctggggctc ggcgtgggca gcgcatccgc gctcgtccct
     3661 tcctacatct cggagtcggc cccgaccgac gttcgcggct cgctctccac gctctttcag
     3721 cttgcgatca ccataggaat cctggtcgcc tacctggtca acgcggcttt cgccaccgcc
     3781 ggcgactggc gctggccggt cgggctggcc gtgattcccg cgctggtgct gttcgtcggc
     3841 atgtacttct tgccggatac tccccgctgg ctcgtcagca agggccggga ggaggaagcg
     3901 cggcgggtcc tgcgccgtac ccggaccgat gaagaggtgg agcgcgagct gaacgacatc
     3961 cggcgcattg aggaagcgga ggaagagcag gccggctaca gagaactgct cgctccctgg
     4021 gtacgtccga tgctcgtggt gggcataggg ctggccgtct tccagcagtt cgttggcatc
     4081 aacaccgtca tctactacgc gcccaccatc atcaagtcca cgggacttgc ggacgtggcc
     4141 tccgtcctcg ccaccgtcgg catcggtgtg gtcaacgtgc tgatgaccgt cgtcgccata
     4201 ctcgtgatag accgggtagg acgcaagccc ttgctcctga tcggtctcgc cggcatggtc
     4261 atatcgctgg ggatcatcgg tggcgcattc gcgttctccg gtctctccgg catcatctcc
     4321 tgggtaacgc tgatagggct gatgctctac atcgcgtctt tcgcggtcag cttcggtccc
     4381 ctgctgtggg tgatgctgcc ggagatcttc ccgctcaacg cccgcgggtc cggcacgggc
     4441 ctatcggcgc ttagcaactg ggtcgccaac ttcatcgtcg cccaggcgtt cctgccgctg
     4501 gtcgggctca taggccgatc cgcggtgttc tggatactcg ccgggatctg cgtcatagcg
     4561 gcgctgttcg tccacttcgt ggtcccggag acgaggggac gcagcctgga gcagatagag
     4621 gccgacctgc gcggagaccg cgaggctgcc gccaccacgt aatattagag acgggggtta
     4681 gagacaaggg cgcggctcgt cccggcagag acggcccgtt ggagataaca tcggggcatg
     4741 tccgcgatcc tgagatctgt gctctccatg tccgccgcca cgggcctctc gcgcctgacc
     4801 gggttcctgc ggacgacggt gatggcggcg acgctcggcg gggcggtggt ggcgaacgcc
     4861 tacaccgtgg cgaacgtgct gccgacccag atctacgagc tgttcatggg cggcatcctc
     4921 tcctcgacgt tcatcccgct gctggttgac cggctcaccc gtcacggcga ggaggacgcc
     4981 cgccgcctga cgaacgccct cgtgacggtg gtgctgccgt tcctggccgt cgtcgcgctg
     5041 ctcggcgtgg tattcgcgcc gcagatagtg gcgctcacca ccgactgggg cgcggccgag
     5101 aagctctcgc cggaggaggc gcggcggacc acggagcttg ccgtgctcct ctttagggtg
     5161 atggccgtcc agatcttctt ctacggcgcc ggagcgatct cgaccggcgt cctcaactcc
     5221 caccgccgct tcttcctccc taccttcgcc cccgtcctca acaacctcat cgtcatatcc
     5281 tccctcgtcg gatacgccct gctggcgcgg aaggacttca ccgccgccat ctacatcctc
     5341 gccggcggca ccaccctcgg cgtcgccgtc atgtccctgg tcctcgtccc ggcggtcctg
     5401 aagctcggct accggccccg cccggtgctc gggcaccccg ccctcgccga cgccgtaaag
     5461 ctcgccctgc cgatgctcgt cttcgtggcg gcgtccgtcg gcgtgcaggt cgcggcctac
     5521 cgcttcggga cgcagttcag cgcggcggcg cagctcctgt acgccttcgt catcttccag
     5581 ctcccctacg ggatcttcgt cgtctcaatc ggcaccgccc tcatgcccga gctctccgag
     5641 cgctacgcca gcgaagacac cgacggctac cgcgctaccc tctccttcgg tctgcggacg
     5701 atggccttca tcgccgtccc cgcctccgtc ggcatggtcg ccctggcgca gcccatcgtc
     5761 ggcctgctct acgaacgcgg ccaattcacg ccccaggaca ccgcgaccgt cgccaccctc
     5821 ctcgtcgcct acggcgtcgg cctgcttggt tacggagcct acttcgtgct cgtccgctcc
     5881 ttctactccc gccagaacac aaggaccccc gccaccctca acctcgggct cctcgtcctc
     5941 tacatagccc tcgcctacac cctctccgct acggtcgggc tcatcggcgt cgccctcgc
//
LOCUS       DASSMB010000134         2098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1676345, whole genome shotgun sequence.
ACCESSION   DASSMB010000134 DASSMB010000000
VERSION     DASSMB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1676345"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFJ72_08155"
     CDS             complement(<1..449)
                     /locus_tag="VFJ72_08155"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET7479470.1"
                     /translation="MRKADTLGLWSWVLVLAAGGIVLLSFGINVSLGAGTLERISTDS
                     MDIHADFDTFWRSADAMWSGRDVYEAGARLVNLNPPFWTVVLAPFGLMEPLTAYRVFI
                     MISLFITVGYLAWMAEELKLRAGWTALAMGMLLLSSPLLATLALGQM"
     gene            complement(515..604)
                     /locus_tag="VFJ72_08160"
     tRNA            complement(515..604)
                     /locus_tag="VFJ72_08160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(568..570),aa:Ser,seq:cga)
     gene            complement(860..>2098)
                     /locus_tag="VFJ72_08165"
     CDS             complement(860..>2098)
                     /locus_tag="VFJ72_08165"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565794.1"
                     /note="Catalyzes the rate-limiting step in dNTP synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     alpha"
                     /protein_id="HET7479471.1"
                     /translation="NYPLPEIDAMSRGNRRIGLGVMGFADLLIKLGITYDSEEGLAFA
                     EKVMKFVDDEAWEASRALAEERGVYGNFEGSRHEARGDRVRNATVTTIAPTGTISIIA
                     GCSGGIEPLFAVAFMRRQADMELPDVNPEFVRLAKARGFYSEELMKKVAEHGSVRDIE
                     EVPEDVRGTWVTSHDITSEWHVRMQAAFQKYTSMGISKTINLPNEATTKDVEDAYRFA
                     FSLGCKAMAVYRDGSRDAQVLSTGKTRVGVQPGTEVEKEYVSLAKARNGRPRTLSGVT
                     KKIQTGHGPLYVSMNDDEFGPRECFIVIGKPGGTAAAFSDALGRMISLAEKHGATPAE
                     IVHQLRGIQDGHPAGFGANAILSVPDGVAKAMAEHYLVEEAPEAKNLPVIGACPECSG
                     GLTKQEGCVVCHSCGYSKCS"
BASE COUNT          373 a          678 c          667 g          380 t
ORIGIN      
        1 tacatctgtc cgagcgcgag cgtcgccaag agcggcgaag agagcaggag catccccatc
       61 gcgagcgccg tccatccggc gcgcagcttc aactcctcgg ccatccacgc caggtaaccg
      121 acggtgatga agagtgagat catgatgaag accctgtacg ccgtcagcgg ctccatgagc
      181 ccgaacgggg cgagcaccac cgtccagaac ggcggattca ggttgacgag ccgcgctccc
      241 gcctcgtaca cgtcccgccc gctccacatc gcgtccgccg agcgccagaa agtgtcgaag
      301 tccgcgtgaa tgtccatgga gtcggtggag atcctctcca gagtcccggc ccctagagac
      361 acattgatcc cgaacgataa cagcacgatc ccaccggcag ccagaaccag cacccaactc
      421 catagcccca gagtgtcagc cttacgcacc gctaataccg tccatacccc ggcatgatac
      481 gcctcagcaa gaatggattc tcagaaacaa agagcggaga gggggggatt cgaacccccg
      541 atgccgggtt taccgacata acggttttcg agaccgccgc attcaaccgc tctgccacct
      601 ctccgcgcga gagtgtaccg catgaacaca ctgctttcag cagtataact ctgagtcttc
      661 agacgacgac agccgttgat agcattccat ctaccctgga cttctagttt attgttatcc
      721 gctctgtctc ggcgatgcga cttccaggac aacccggaac gatttattgc gatttgcagg
      781 tacttcgttc gtgtggcaaa ggaaaaggcg ggcctttcgg cccgccatgc ctaaccctgc
      841 ctgcaagggt taaccagggt tagctgcact tggagtagcc gcaggagtgg cagactacgc
      901 atccttcctg cttggtgagg ccgccggagc actcggggca ggccccgatg acggggaggt
      961 tctttgcctc gggcgcctcc tcgacgaggt agtgctcggc catcgccttc gccacgccgt
     1021 cggggacgga gaggatggcg ttggcgccga agcccgccgg gtggccgtcc tggatgccgc
     1081 gaagctggtg gactatctcg gccggggtgg cgccgtgctt ttcggccaga gagatcatgc
     1141 gccccagagc gtcgctgaac gccgccgccg taccgccagg cttgccgata acgatgaagc
     1201 actcgcgcgg gccgaactcg tcgtcgttca tcgagacgta gagcggaccg tggccggtct
     1261 gtatcttctt ggtcacgccg gagagcgtgc gcggccggcc gttgcgcgcc ttggcgagcg
     1321 agacgtactc cttctcgacc tcggtgccgg gctgcacacc gacgcgtgtc ttgccggtgg
     1381 agaggacctg ggcgtcgcgc gagccgtcgc ggtagacggc catcgccttg cagccgagcg
     1441 agaacgcgaa gcggtaggcg tcctcgacat ccttggtcgt cgcctcgttc gggaggttga
     1501 tggtcttgga gatccccatc gaggtgtact tctggaacgc cgcctgcatc ctgacgtgcc
     1561 actcgctcgt gatgtcgtgc gaggtgaccc aggtgccgcg cacgtcttcc gggacctctt
     1621 cgatgtcgcg gacggagccg tgctcggcga ccttcttcat gagctcctcg gagtagaagc
     1681 cgcgagcctt ggcgagccgc acgaactcgg ggttcacgtc cgggagctcc atgtcggcct
     1741 ggcggcgcat gaacgccacg gcgaagagcg gctcgatgcc gccggagcag ccggcaatta
     1801 tggagatggt gccggtcggg gcgatggtgg tgacggtcgc gttgcggacc cggtcgccgc
     1861 gggcctcgtg gcgcgagccc tcgaagttcc cgtagacgcc gcgctcctcg gcgagcgcgc
     1921 ggctggcctc ccaggcctcg tcgtccacga acttcatcac cttctcggcg aaggccagac
     1981 cttcctcgga gtcgtaggtg atgccgagct ttataaggag gtcggcgaag cccatcacgc
     2041 cgagcccgat gcggcggttg ccccggctca tcgcgtcgat ctccgggagc gggtagtt
//
LOCUS       DASSMB010000135         5708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_663301, whole genome shotgun sequence.
ACCESSION   DASSMB010000135 DASSMB010000000
VERSION     DASSMB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663301"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /locus_tag="VFJ72_08170"
     CDS             <1..1104
                     /locus_tag="VFJ72_08170"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET7479472.1"
                     /translation="FRLCRTSGMWLAVAEEVLRLTDETRKNFYNGERVYLEGEEMRFP
                     ELADTLDAIGQEGADLFYEGRLGRAVAAYVKEMGGIITERDLAEYRPVIREPLAMRYG
                     HGEIFTNCPPSAGGATLAQMLQIVSAYDLANLPEEEFVRVMAGAMKYALADRETAYIE
                     GEGNAKVAERLTSEEYAKRQRERIFGAFGSPHTTHLSCVDAEGFAVSITASMGYGSGL
                     VVPGTGIPLNNTLGEPELNPSGFHALKPGEKLVSSMSPTIVSSEEGGLVSLGSPGASR
                     IPTAILQTLVNVLDLGMPLESAVLAPRFHAEGDLFAYEAGARKADLERYGRVLPYDGP
                     SMFFGGVNAVRRTPEGLFEAAADPRRSGGAVFV"
     gene            1097..2026
                     /gene="rocF"
                     /locus_tag="VFJ72_08175"
     CDS             1097..2026
                     /gene="rocF"
                     /locus_tag="VFJ72_08175"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HET7479473.1"
                     /translation="MSEEAERARRPVGIFGVPMDLGQGRRGVDMGPSAIRYARLETSL
                     GDLGYHVTDLGNAAVPIPETVEKEEEVRHIGAVRQVCEAVADRAGAMVSEGLFPVFLG
                     GDHSIAIGTVSGVAGAFESRTGVIWLDAHADFNTNDTSPSGNIHGMPLAALAGRGHPD
                     LVNIGGPGASVRAEDVVIIGLRSVDQQERELLREAGVRSYTMKEIDVYGVAQVVRQAI
                     KDLSHLDRVHLSFDLDSVDPDVAPGVGTPVRGGLTYREAHLVMELINESGIVTSLDVV
                     EVNPILDERNGTAELAVELVASLMGRQIIGLPD"
     gene            complement(2023..3486)
                     /locus_tag="VFJ72_08180"
     CDS             complement(2023..3486)
                     /locus_tag="VFJ72_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET7479474.1"
                     /translation="MNPRVIIRVNAGVVTAIGLAMLVPLALSLLYADGSWTSFLIPSL
                     VLIPAGAATIWASGPLGRRAEIISNRDVYLSVTLAWTLAALVGGVPFLIEGTFSSVVD
                     SAFETMSGFTTTGATLLSDIEAQTPSILFWRSMTHWLGGIGIVVLFVAVAPLLGVGAA
                     RLLGAEMSGLTQPRLTPRIADTAKALVVIYLAISAAETLALLLAGMSLYDAVLHTFGT
                     VATGGFSNRTASIAYYDSAAIEIIIVFFMTISGVSFALYYLLYTRRRLSVVLDRELLA
                     YLGIIAGATIFVWGVLVLEGDYGGGVARALRDSLFAVSSVITTTGFVTADFDEWDTAA
                     KITLVLLMFIGGCAGSTAGGLKVIRVIIVFRTILQDVFKMIHPRAMTPLKIGGTVVPE
                     SVRIAVLGLFAAWVGVFGIATFMVALQENLTPLSSATAVAATLNDVGPGLGQVGATES
                     FEIVNPFGRVVLTVCMLLGRLEIFTALALLSPAFWRR"
     gene            complement(3490..4824)
                     /gene="trkA"
                     /locus_tag="VFJ72_08185"
     CDS             complement(3490..4824)
                     /gene="trkA"
                     /locus_tag="VFJ72_08185"
                     /inference="COORDINATES: protein motif:HMM:NF007032.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET7479475.1"
                     /translation="MRTVIIGAGEVGFNTARMLSHEGHDVVLIEQDETLVERATEQLD
                     ALVIHGNGASPKLLSEAGIQRSELLVAASSSDEANIIACLAAKAQGVPRTVARIHNPD
                     YYNPKEPFARDMLGIDFVIHTEQMAAEEIKKALLVPGAINVETFAEDSIEVAEVVLHD
                     DSPVAGKALREVRLPEGSLVVGVVRGGEALVPRGETVLELRDHVLLISARRGVSDVVK
                     AVTTDTAPVKDVTIYGGGRIGLRLALALEKVGMFVRVIERDEARARYVASLLRKGFVL
                     HDEGISRDFLLQERVDRTDAFVAVTADDRANLLAAMYARQLGARTTIAGVSRGEFAPL
                     AHALGVDLTISPRMLAAEAILRFVRKGEVINVALLESGAEMIELRIPESSRVAGRPLS
                     EVGFPEGAIVGAILRDGAVVIPTGREVLRPGDDAVVFTVEGAVEEVERLFAP"
     gene            complement(4837..5508)
                     /locus_tag="VFJ72_08190"
     CDS             complement(4837..5508)
                     /locus_tag="VFJ72_08190"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET7479476.1"
                     /translation="MSKQFVVIGLGRLGSVMAKTLDELGHTVLAIDQDETLIQNLTDD
                     LPHVHLVAADATDEAVLRDLGVQNFDGAAVVIGGNIQAGILATVLLKELGVPHVISRA
                     SSATHAKVLDKIGADRVIQPEKEMGAQVARTLASPAVLDYVDLGGDEAIIETEVPEKW
                     LNHSLAELQLSRKHGLTVLALKAKGQAGTIPRGDTVLHEGDVMVVGGSKKNLDRSDLL
                     QSRDR"
     gene            complement(5516..>5708)
                     /locus_tag="VFJ72_08195"
     CDS             complement(5516..>5708)
                     /locus_tag="VFJ72_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HET7479477.1"
                     /translation="FGTVGLSLGPGYGLATELSPFGKLLLMAVMFAGRVGPITVILAL
                     SERARPKRFTYPEEDIAIG"
BASE COUNT          978 a         1890 c         1923 g          917 t
ORIGIN      
        1 ttccggctct gccggacgag cggcatgtgg ctggccgtcg ccgaggaggt cctacggctc
       61 accgacgaga cccgcaagaa cttctacaac ggcgagcgag tgtacctgga gggcgaggag
      121 atgcgcttcc cggagctcgc cgacacgctc gacgccatag ggcaagaggg ggcggacctc
      181 ttctacgagg ggcggctggg gcgagccgtc gccgcctacg tgaaggagat gggcggcata
      241 atcacggagc gggacctcgc cgagtaccgg cccgtcatcc gggagccgct cgcgatgaga
      301 tacgggcacg gtgagatctt cacgaactgc cctccttcgg ccggcggcgc aacgctggcc
      361 cagatgctcc agatagtctc cgcctatgac ctcgccaacc tacccgaaga ggagttcgtg
      421 cgggtgatgg ccggcgcgat gaagtatgcc ctcgcggacc gcgagacagc gtacatcgag
      481 ggcgagggga acgccaaagt cgccgagcgc ctcacgagcg aggagtacgc gaagcgccag
      541 cgggagcgca tcttcggcgc gttcggcagc ccgcacacca cgcacctctc ctgcgtggac
      601 gcggagggtt tcgcggtctc cattacggca agcatgggtt acggatccgg gctggttgtc
      661 cccggcaccg gcatcccgct caacaacacc ctgggagagc cggagctgaa cccgagcggc
      721 ttccacgcgc tgaagcctgg cgagaagctc gtctccagca tgagcccgac catcgtctcc
      781 tccgaggagg gaggactggt cagcctcggg tctccgggcg cctcgcgcat cccgacggcg
      841 atccttcaga ccctggtcaa cgtactggac ctcgggatgc cgctggagag cgccgtgctc
      901 gcgccgcgct tccacgcgga gggggatctc ttcgcgtacg aggccggagc ccgcaaggcc
      961 gacctggagc gttacgggcg cgtgctgcct tacgatggac cttccatgtt cttcggcggc
     1021 gtcaacgccg tgcggcgcac gccggaaggt ctcttcgagg ccgcggccga cccgcgccgc
     1081 agcgggggag cggtctttgt ctgaggaggc cgagagggcg cggaggccgg tcggcatctt
     1141 cggggtgccg atggatctcg ggcagggacg gcgcggggtg gatatgggac ccagcgcgat
     1201 tcgctacgcg cgcctcgaaa cgtcactggg agacctcgga taccacgtca ccgacctcgg
     1261 gaacgcggcg gtccccatcc cggagaccgt agaaaaggaa gaagaggtcc ggcacatcgg
     1321 ggcggtccgt caggtctgcg aggcggtggc cgatagggcc ggcgcgatgg tctccgaggg
     1381 gctgttccct gtcttcctcg ggggggatca ctccattgct atcgggaccg tctccggcgt
     1441 ggccggggcg ttcgagagca ggaccggcgt gatctggctg gacgcccacg cggacttcaa
     1501 taccaacgat acatcgccct ccggcaacat ccacggtatg cctctcgccg ccctcgccgg
     1561 acgcggacac cccgacctgg tcaacatcgg cggtcccggc gccagcgtgc gcgccgaaga
     1621 cgtggtgata atcggtctgc gttcagtgga ccagcaggag cgcgagctgc tgcgcgaggc
     1681 gggcgtgcgg agctacacca tgaaggagat cgacgtctac ggtgtggcgc aggtcgtgcg
     1741 gcaggcgata aaggacctct cgcacctcga ccgggtgcat ctctccttcg accttgacag
     1801 cgtcgacccg gacgtggcgc ccggcgtggg aaccccggta cgcggcggcc tgacttaccg
     1861 ggaggcgcac ctggtgatgg agttgatcaa cgaatccggc atcgtgacct cgctcgacgt
     1921 ggtggaggtc aaccccattt tggacgagcg caacggcacg gcggagctgg ccgttgaatt
     1981 ggttgcgagc cttatgggac gccagatcat cggcctgccc gactagcgcc tccagaacgc
     2041 cggggagagc agggcgaggg ccgtaaagat ttcgagacgc cccagcagca tgcagaccgt
     2101 gagtaccacc cggccgaacg gatttactat ctcgaagctc tccgtggcgc cgacctggcc
     2161 gagtcccggc ccgacgtcgt tgagggtggc cgcgaccgcc gtcgccgagg agagcggtgt
     2221 cagattttcc tgaagggcga ccatgaacgt cgcgattccg aacactccga cccaggcggc
     2281 gaaaagcccc aggacggcga tacgtacgct ctccgggacc acggtgccgc cgatcttcag
     2341 cggcgtcatt gcgcgggggt ggatcatctt gaagacgtcc tggagtatcg tgcggaagac
     2401 gatgatgacc cggatgacct tcaggccgcc cgccgtcgag ccggcgcagc ctccgatgaa
     2461 catcagtagt acgagagtta tcttggcggc cgtatcccac tcatcgaagt ccgcggtgac
     2521 gaagccggtg gtggtgatca cgctggagac ggcgaagagc gagtcgcgca gcgctcgcgc
     2581 cacgcccccg ccgtagtccc cctccaggac gaggacgccc cacacgaaga tcgtcgcccc
     2641 cgcgatgatg cccaggtagg ccagaagttc ccggtcgagg accacgctca ggcgccgccg
     2701 ggtgtagagc aggtagtaga gtgcgaaaga gaccccggag atcgtcatga agaagacgat
     2761 gatgatctcg atggccgccg agtcgtagta ggcgatggag gcggtcctat tactgaaacc
     2821 gcccgtcgcc accgtgccga aggtgtgcag gacggcgtcg tagagcgaca tcccggccag
     2881 aaggagcgcc agggtctccg ccgcggagat tgccaggtag atgacgacca gggccttcgc
     2941 cgtatccgcg atgcggggcg tgagccgcgg ctgggtgagt cccgacatct ccgcgcccag
     3001 caaccgggcc gccccgacgc cgagcagcgg ggccacggcg acgaagagca ccacgatccc
     3061 gatgccgccc agccagtggg tcatgctgcg ccagaacagg atcgaaggcg tctgcgcctc
     3121 tatgtccgag agcagtgtgg ccccggtcgt ggtgaagccg ctcatcgtct cgaacgccga
     3181 gtcaacgacg ctggagaacg ttccctctat aaggaacggc acgccgccga cgagcgcggc
     3241 gagcgtccac gccagggtca cggagaggta tacgtcgcgg ttcgagatga tctcggcgcg
     3301 gcgtcccagc gggccggaag cccagatggt cgcggctccg gcggggatca agacgagcga
     3361 cgggatcagg aagctggtcc acgagccgtc ggcatagagc agagagagcg cgagcgggac
     3421 gagcatggcg agcccgatgg ccgtaacgac cccggcgttg actcgtatga tgaccctggg
     3481 gttcacgggt cagggggcga agagccgctc gacttcttcc acggctccct cgacagtgaa
     3541 gacgaccgcg tcgtctccgg ggcgcaggac ctccctgccg gtcggtatca ccacggctcc
     3601 gtcccgcagg atcgctccga cgatggcccc ctccgggaaa cccacctccg agagcggccg
     3661 gccggcgacg cgggagctct ccggaatccg cagttcgatc atctccgcgc cgctctccag
     3721 gagggcgacg tttatgacct cgccctttcg cacgaaccgg agaatcgcct cggcggccag
     3781 catgcgtggg gagatcgtca ggtccacgcc cagcgcgtgg gcgaggggag cgaactctcc
     3841 ccggctgaca cccgcgatgg tggtccgggc cccgagctgg cgggcgtaca tcgccgccag
     3901 caggttcgcc cggtcgtcgg cggtcaccgc gacgaaggcg tcggtccggt ccacgcgctc
     3961 ctggagcagg aagtcccgcg agatgccctc gtcgtgcagc acgaatccct tgcgaagcag
     4021 ggaggccacg tagcgcgcgc gggcctcatc gcgttcgatc acccgcacga acatcccgac
     4081 tttctccagc gcgagcgcca gccgcagacc gatgcgtccg ccgccataga tcgtcacgtc
     4141 tttcaccggg gccgtgtcgg tcgtaaccgc cttcaccacg tccgagacgc cgcgccgggc
     4201 gctgatgagc aggacgtggt cccgcagctc caggacggtc tccccgcggg ggacgagcgc
     4261 ctctccgccg cgcaccaccc cgaccaccag gctcccctcc gggagtcgca cctcccggag
     4321 ggccttcccg gcgaccggcg aatcgtcgtg caggaccacc tcggcgacct ctatgctgtc
     4381 ctcggcgaaa gtctccacgt tgattgcgcc cggcaccagc agggccttct ttatctcctc
     4441 cgcggccatc tgttcggtgt ggatgacgaa atctatcccg agcatgtcgc gggcgaatgg
     4501 ttctttggga ttgtagtagt ccgggttgtg gatacgggcc acggttcggg gcacgccttg
     4561 cgccttcgcc gccaggcagg cgatgatgtt cgcctcgtcg gagctgctcg cggccacgag
     4621 gagctccgac cgctggatgc ccgcctcgga gagcagtttg gggctggcgc cgttaccgtg
     4681 gatcaccagc gcgtcgagtt gctcggtggc gcgctccact agcgtctcgt cctgctcgat
     4741 cagcacgacg tcgtggccct cgtgggagag catgcgggcg gtattgaacc cgacctcgcc
     4801 ggcgccgatg atcacggtcc gcacggcggc gcctctttac cggtcccggg actgcaagag
     4861 gtccgatctg tccaggttct tcttcgatcc tcccacgacc attacgtcac cctcgtgcaa
     4921 aacagtgtcc ccacggggga tcgtaccggc ctgtcccttc gccttgagcg ccagtaccgt
     4981 gaggccgtgc ttgcgggaga gctgcaactc ggcgaggctg tggttcagcc acttctccgg
     5041 tacctctgtc tctataattg cctcgtcccc tccgaggtcc acgtagtcta ggacggccgg
     5101 agaggccagc gtgcgcgcga cctgggcgcc catctctttc tctggttgga tgacgcggtc
     5161 cgccccgatc ttatccagca ctttcgcgtg cgtggcgctc gacgctcggg agataacatg
     5221 cggcacgccg agttccttta atagaaccgt agccaggatg ccggcctgga tattccctcc
     5281 gatgacgacc gccgccccgt cgaagttctg gacgcccagg tcgcgcagga cggcctcgtc
     5341 tgtcgcgtcg gcggcgacga ggtggacgtg ggggaggtcg tccgtgaggt tctgaatcaa
     5401 ggtctcgtcc tggtctatgg cgagcaccgt gtgcccgagc tcgtccaggg tcttggccat
     5461 caccgaaccg agccgcccca acccgataac gacgaactgc ttgctcatag cgtgctcatc
     5521 ctatagctat atcctcctcc ggatacgtga accgtttggg ccgggcgcgc tcgctcaggg
     5581 cgaggatgac cgtgataggt cctacccgtc ccgcgaacat caccgccata agcagcagtt
     5641 taccgaacgg gctgagctcc gtggcgagac cgtagccggg tccgagtgac aggccgaccg
     5701 tcccgaac
//
LOCUS       DASSMB010000136         2032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_663349, whole genome shotgun sequence.
ACCESSION   DASSMB010000136 DASSMB010000000
VERSION     DASSMB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663349"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..>2032)
                     /locus_tag="VFJ72_08200"
     CDS             complement(235..>2032)
                     /locus_tag="VFJ72_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HET7479478.1"
                     /translation="IDAGGGIWLVQARPITTLFPLPANAPATDDAPRVYFSGSVAQGV
                     YQPLTPMGQQAFRLVASALSTLLGYQPSDRYAGPGPYAEAAGRLFVDITPVLRSTFGR
                     RLLDRVMRNMETRTAPILLHLADDPRLSPVPTPWPKILRALVPLLVRKRIPPRVVGAL
                     ARPATARARAARVAAEFRTAGDTYRNAGTAERLDAAERLLSGFPPRLLPGVPPTFIAG
                     LIANAIARKLLGDLATEDERRVVLRALPHNPTTEMDLHLWRLAKRVREDPDAAQKVRE
                     TPPDLLAEEYREGVLPPDLQTGLADFLRFYGHRGVAEIDLGQPRWSEDPTHILGVLAN
                     YLRLQDPDLAPDVQFRRAEREAESMVVELTRRAARKGRIRGKLVGFFLRRTRALMGMR
                     EMPKFCIIVLFAQVRALLWSVGEDLATTGLLESAEDIFFLSLPEAWSAVGGEDLRSLI
                     RERRADYEQETRRRHVPRILLSDGTAPDVSAQDSPDSAKGVLSGTPASGGVVSGRARV
                     VLDPAGAHLEPAEILVAPSTDPGWTPLFLTAGGLVMEMGGPMSHGAIVAREYGIPAVV
                     GVPDATERITTGQEITVDGSAGTVSLDQQSDS"
BASE COUNT          317 a          662 c          731 g          322 t
ORIGIN      
        1 gtctcctgcg gcgcggagaa atggggagca ggcgaggtag tgagcgtccc gatactcgac
       61 caggacggta atcagcagga tgattcgtgc gttccgatgg tccaactcgt ctgtaccaac
      121 tgcagctacc tgatgctcta cgccgccgtg cccatcggtc tgccgtagta tatacgctcc
      181 ggcatgcggg aaagtccgca cagagccgtg tcgtggatcg ctaccgtgcg tggctcacga
      241 gtcgctttgc tgatcgagcg agaccgtccc ggcggagccg tccaccgtaa tctcctgtcc
      301 cgtggtgatg cgttcggtgg cgtccggtac tccgacgacg gctgggatgc catactctcg
      361 ggcgacgatg gccccgtggg acatcggccc gcccatctcc attaccaggc cgccggccgt
      421 gaggaagagc ggcgtccagc cgggatcggt cgagggcgcg accaggatct cagcgggctc
      481 cagatgggca ccggcggggt cgagcaccac ccgcgcccgg ccgctcacca cgcccccgga
      541 ggcgggagtc ccgctcagga ctccctttgc tgagtcgggc gagtcttggg cgctgacatc
      601 cggcgcggtg ccgtcggaga ggaggatgcg cggtacgtgg cggcggcgcg tctcctgttc
      661 gtagtcggct cgccgttcgc ggatcaggga ccggaggtct tcgcctccca cggccgacca
      721 cgcttcgggc agggacagga agaagatatc ctcggcgctc tccaggaggc cggtcgtcgc
      781 caggtcctcc ccgacgctcc acagcagcgc ccgaacctgc gcaaagagca cgatgatgca
      841 gaacttgggc atctcgcgca tccccatgag ggcccgtgtc ctccgcagga agaagccaac
      901 cagcttcccc cggatgcggc ccttgcgggc ggctcggcgg gtgagctcga cgaccataga
      961 ttcggcctcc cgctccgccc ggcggaactg cacgtccggg gccagatccg ggtcttgcag
     1021 acggaggtag ttagccagca cgcccaggat gtgcgtgggg tcctcggacc agcgcggctg
     1081 gcccaggtcg atctcagcca ccccgcgatg tccgtagaag cgcaggaagt ccgccagccc
     1141 tgtctggagg tccggcggca gcacgccctc ccggtattct tcggccaaca gatccggcgg
     1201 cgtctcgcgc accttttgcg cggcgtccgg gtcttcgcgc acccgtttgg cgagccgcca
     1261 gagatgcagg tccatctcgg tcgttggatt gtgcggcagc gcgcgcagca ccactcgccg
     1321 ttcgtcttcg gtggcgaggt ccccgaggag ctttctggcg atggcgttgg cgatgagacc
     1381 ggcgataaat gtcggaggaa cgccgggtag caggcgcggc gggaagccag acagcaggcg
     1441 ttcggccgca tccaggcgtt ctgcggtgcc ggcgttccgg tacgtatctc ccgctgtacg
     1501 gaactccgcc gccacgcgcg ccgcgcgggc gcgggccgtg gcaggacgcg cgagcgcccc
     1561 gacgacgcgg ggcgggatgc gtttccggac gagcaggggc accagggccc gcaggatttt
     1621 cggccacgga gtgggtacgg gggagaggcg tggatcgtcg gcgagatgca gcaggatcgg
     1681 ggcggttcga gtttccatgt tgcgcatcac ccggtcgagg agcctgcgtc cgaacgtgct
     1741 tcgcagcacc ggggttatgt cgacgaagag ccggccggcg gcctcggcat aggggcccgg
     1801 accagcgtac ctgtcggacg gttgatagcc gaggagcgtg gagagcgccg atgccaccag
     1861 ccgaaacgcc tgctgcccca tcggtgtcag cggctggtag acgccctggg cgacgctgcc
     1921 cgagaagtag acgcgcgggg cgtcgtcagt cgccggggca ttggccggga gagggaagag
     1981 ggtcgtgatc gggcgcgcct ggaccagcca aattccaccg ccggcgtcga tg
//
LOCUS       DASSMB010000137         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1297562, whole genome shotgun sequence.
ACCESSION   DASSMB010000137 DASSMB010000000
VERSION     DASSMB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297562"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VFJ72_08205"
     CDS             <1..773
                     /locus_tag="VFJ72_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479479.1"
                     /translation="SDNRAVLIENKIYAMVNNPLADYTDYLDCRILDGRAKHKLLLTV
                     FPTGEGSEWNFVNLTYSKFVDEIRSLLGHYLFGADTRYLTMFLDFLSTLENLRRGTRM
                     DQEFVKFLAKRDDDIRNLFNDLKSFRIEIREKVLELRTLIDTSQHETVEVQDLWRGDT
                     ASMSDTLYHNIRVSEDLLVGVDTRLNPQGWQIQIFARSKGSTLKLKELLKRLEIPFEE
                     RERFIHPAQFDYDENLDRISPVLQDIVNKLATSQENEA"
     gene            829..2355
                     /locus_tag="VFJ72_08210"
     CDS             829..2355
                     /locus_tag="VFJ72_08210"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7479480.1"
                     /translation="MEAVLNDFVQVLRRHRLRVSPAESLDALHALEQVGIGEREVVRD
                     TLRATLIKNLDDVEAFDKLFDLFFSLRPEPERKTAKLELPGHDHDHGPPPARMEFGEE
                     AAGQAPESEEHSHEDKESTEFRRFFDDERMRPSRDFHGEADRMRLSMFSQELILNRKP
                     GALNQAMQRITHQLRLRRARNIFNPGDLVPHSEGQELPLDVSAAELGELIDHLHELEV
                     DEALIRQLEAQSDDILRSLPQLIEQMVKRQKELDSEKDDSALRRRSLQKMLDFSPSEQ
                     REMEAAIRRIARQIHGAKTRRLKQDRTGHISVPHTLRQNIRYEGIPFHPVLRRHHEKR
                     PRVAMLCDVSLSTRNLSRFWLHMVYQMQSLFSKVRTFVFVAEMAEVTQLFEENTMNGA
                     VEEIFSGRVLDADVNSDFGRAAEQFVGEHLSSINHRTTVVILGDGRNNGKPPNTEALE
                     EISHHARQVIWITPEPRWGWALGGCDMPLYEEVCGRVEVVRTVDQLAGIAEELVRNRV
                     "
     gene            2352..2888
                     /locus_tag="VFJ72_08215"
     CDS             2352..2888
                     /locus_tag="VFJ72_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7479481.1"
                     /translation="MTGGSGEVPFSPTDPIRYCPADGTQLGEARASGGVTCPECGRSW
                     YRNSSPTVGAAIVKDGRALVTVRGIEPEKGKVDVPGGFLLVGEHPVDGLRREILEELG
                     VEAEVEDNPIMLATHTYGEGGIWVLAIGFKARIEGEPRPADDVAEIRWVSPEEVDGLD
                     FAWEHDREFVRAALADVV"
     gene            2927..3682
                     /locus_tag="VFJ72_08220"
     CDS             2927..3682
                     /locus_tag="VFJ72_08220"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479482.1"
                     /translation="MGAIDGWLEGSMHGQAGVGVILLISLLLGLRHASDPDHLAAVTT
                     LIASEEQNHVRKAGIMGFLWGLGHGTTLVLVGLPLVLLNRYLPEVISQIAEVAIGCII
                     VLLAVRLLMRWRRGFYHVHVHTHSSGEPHRHVHTHVEDSSHRHGHRVPQRTPLSSYGI
                     GLVHGVGGSGGLTLLLLSTISDKTQATVALLIFAAGTAVSMAVLSTVFGLVIAGGPIA
                     RNFERVAPVLGVLSMAFGFWYALGALGLVAYPF"
BASE COUNT          764 a         1134 c         1197 g          682 t
ORIGIN      
        1 agtctgacaa ccgggctgtc cttatcgaga acaaaatcta cgcgatggta aacaaccccc
       61 ttgccgacta cacggactat ctcgactgtc gaatactcga tgggcgtgcg aaacacaagc
      121 tccttcttac agtctttccg acaggcgaag gcagcgaatg gaattttgtg aacctcacgt
      181 actcgaaatt tgttgacgag atacgctcgc tgctcggtca ttacctattc ggtgcggaca
      241 ctcgttacct gacgatgttt ctagattttc tgagcacact cgaaaacctg cgaaggggta
      301 cccgcatgga tcaggagttc gtcaagttcc tcgcaaaacg ggatgatgac atcagaaacc
      361 tctttaacga tcttaagagt ttcagaattg aaatacggga gaaggttctg gagttgcgga
      421 cccttataga cactagccaa catgaaactg ttgaggtgca agatctctgg cgaggagata
      481 cagcatctat gtctgacact ctataccaca acattcgtgt atccgaggat ttgctggttg
      541 gcgtagacac tcgcctcaac ccgcaaggtt ggcagattca gatctttgct cgtagcaaag
      601 gcagtacttt aaaactgaaa gaactcctga aacgcttaga aattccattt gaggaaagag
      661 aacggttcat tcatcctgct caattcgatt acgacgagaa tctagatcga atcagtccag
      721 tccttcagga tattgtcaac aagcttgcca cgagccagga aaacgaggcg tgattttggc
      781 tcaatctgac aaactattct attttgcaca cagtaaggga gcgtgctgat ggaagccgtc
      841 cttaacgact tcgtgcaggt gttgaggcgg caccggctgc gggtctctcc ggccgagagt
      901 ctggacgccc tgcacgcgct ggagcaggtg ggcatcggtg agcgggaggt cgtgcgggat
      961 acgttgcggg ccaccctgat caagaacctc gacgacgtgg aagctttcga caaacttttc
     1021 gacctgttct tcagcctgcg cccggagccg gagaggaaga cggcgaagct ggagttgccg
     1081 ggccacgacc acgatcacgg tccgccccct gcgcgtatgg agttcgggga ggaggccgcc
     1141 gggcaggcgc cggagtccga ggagcacagc cacgaggata aggagtcgac cgagttccgg
     1201 cgcttcttcg atgatgagcg gatgcggccc tcgcgcgact tccacggcga ggccgaccgg
     1261 atgcgcctct cgatgttctc gcaggaattg atcctgaacc gcaagccggg cgcgctgaac
     1321 caggcgatgc agcgcatcac gcaccagcta aggttgcgcc gggcccgcaa catcttcaac
     1381 cccggcgacc tcgtccccca ctccgagggc caggagctgc cgctggacgt ctccgccgcc
     1441 gagctcgggg agctcataga ccacctgcac gagctggagg tggacgaggc cctgatccgg
     1501 cagctcgaag cccagtccga cgacatcctg cgcagcctgc cccagctcat cgagcagatg
     1561 gtgaagcgcc agaaggagct cgacagcgag aaggacgact ccgcgctccg gcggcgctcc
     1621 ctccagaaga tgctcgattt ctccccctcc gaacagcggg agatggaggc cgccatccgg
     1681 cggattgcgc gccagatcca cggcgccaag acccgccgcc tcaaacagga ccgcaccgga
     1741 cacatcagcg tcccccacac cctgcgccaa aacatccgct acgagggaat ccccttccac
     1801 cccgtgctgc gccgccacca cgagaaacgt ccgcgtgtcg caatgttatg cgacgttagt
     1861 ctaagcaccc gcaacctcag ccggttctgg ctgcacatgg tctaccagat gcagagtctg
     1921 ttctcgaagg tcaggacgtt cgttttcgta gcggagatgg cggaggtgac gcagctcttc
     1981 gaggagaaca cgatgaatgg ggcggtagag gagatcttct ctggccgcgt cctggacgcc
     2041 gacgtgaaca gcgacttcgg ccgggcggcc gagcagttcg tcggcgagca cctctcctcg
     2101 atcaaccacc gcacgacggt cgtcatactg ggtgacgggc gcaacaacgg caaaccgccg
     2161 aacacggagg cgctggagga gatctcgcac cacgccaggc aggtcatctg gatcacgccg
     2221 gagccccgct ggggctgggc tctcggagga tgcgacatgc cgctctacga agaggtctgc
     2281 ggccgcgtgg aggtcgtccg caccgtggac cagctcgccg gcatcgccga ggagctggta
     2341 aggaatcgcg tatgaccggc ggctccgggg aagtaccctt ctcccccacg gaccctatcc
     2401 gatactgtcc tgcggacggg acgcagctcg gcgaggcgag agcgagcggc ggcgtaacct
     2461 gccccgagtg cggccggtcc tggtaccgga actcctcccc gaccgtcggg gcggcgatag
     2521 tgaaggacgg gcgggcgctg gtgacggtgc ggggcatcga accggagaaa ggaaaagtag
     2581 atgtgccggg aggtttcctg ctggtcggag agcatccggt ggacgggctc aggcgcgaga
     2641 tcctggaaga gttgggagtg gaagccgagg tcgaggacaa tccgattatg ctcgcgactc
     2701 acacttacgg cgagggcggt atctgggtgc tcgccattgg gttcaaagcg cgtatagagg
     2761 gagaaccgcg tccggcggac gacgtggcgg agatacggtg ggtctcgccc gaggaggtag
     2821 acgggttgga cttcgcctgg gagcacgacc gggagttcgt acgcgcggcg ctggcggatg
     2881 tagtctaaga tgatgtaccc tgaacgtatc cagaggtagg aggctcgtgg gcgcgataga
     2941 cggctggctc gaaggctcca tgcacggtca ggcgggtgtc ggggtgatcc tgctcatcag
     3001 cctgctgctt ggtttgcgcc acgcgagcga cccggaccac ctcgcggctg taacgacgct
     3061 catcgcctct gaggagcaaa atcacgtccg caaggccggg atcatgggct ttctctgggg
     3121 gctcggtcac gggaccaccc tggtgctcgt cggtctgccg ctggtcctcc ttaaccgcta
     3181 cctgcccgag gtcatcagcc agatcgccga ggtcgccatc gggtgcatca tagtgctgct
     3241 ggccgtcagg ctgctgatgc gctggcggcg cggcttctac cacgtccacg tccacactca
     3301 tagcagcggc gagcctcacc gccacgtcca cacacacgtc gaagactctt cgcaccgcca
     3361 cggccaccgc gtgccgcagc gcacgcccct gtcgtcctac ggcatcgggc tggtgcatgg
     3421 cgtgggaggt tcgggcgggc tcacactgct cttgctctcc accatctcgg acaagaccca
     3481 ggcgaccgtg gcgctcctga tcttcgccgc cgggaccgcc gtctcgatgg cggtcctctc
     3541 gacggtcttc ggcctcgtca tagccggcgg ccccatagcg cgcaacttcg agcgcgtggc
     3601 gccggtgctg ggcgtcctga gcatggcgtt cgggttctgg tacgcgctcg gggcgctggg
     3661 actggtcgcg tatccgttct agcggctgct acactgcggg acagggaaaa gcccaatcaa
     3721 ggaggaggaa gagatgccga agtacgtgat cgagcgcgag gtgccgggag ccgggag
//
LOCUS       DASSMB010000138         3875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1677491, whole genome shotgun sequence.
ACCESSION   DASSMB010000138 DASSMB010000000
VERSION     DASSMB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1677491"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..603)
                     /locus_tag="VFJ72_08225"
     CDS             complement(118..603)
                     /locus_tag="VFJ72_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HET7479483.1"
                     /translation="MSWPKIGPLLAAIATCLLFALFGGALVGESLGGWYDELDKPWFL
                     VPLWAFYIVGAVYYVLFITVIYRVIVHVHDRRRRMVSLALTVCVLFLNELWNYVFFDL
                     QSTLAGFLGIAGFLVPLTALLLTLRRYERFSAGLVAVYWIWVLYDLAWAFELWRINGS
                     G"
     gene            complement(617..1699)
                     /locus_tag="VFJ72_08230"
     CDS             complement(617..1699)
                     /locus_tag="VFJ72_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7479484.1"
                     /translation="MNVWVGATLEGVTARMVETPRLATHILESGPEGGVPVVFVHGNV
                     SSALFFEETLVALPDAYRGIAPDLRGFGGSETKSLDATRGVRDFADDLYALVEALGIG
                     RAHFVGWSAGGTVAMQYAMDHPETVPSLALICPMSPYGFGGTKDAAGTPCWPDHAGSG
                     GGTANPEFVKRLAEGDRTDEDPNSPRNVMNAFYFKPPFRAREEREEVYIASMLSTKTT
                     EGNYPGDMTSSENWPSVAPGSRGMNNAISPKYCDLGDFARLEGGPDVLWVRGSDDQIV
                     SDTSLLDFGYLGQLEIVPGWPGPEAYPPQPMVSQTRAVLDEYAARGGGYREEVIPDCG
                     HSPHVEKPEEFRRILLDFLEAHPQGV"
     gene            complement(1696..2175)
                     /locus_tag="VFJ72_08235"
     CDS             complement(1696..2175)
                     /locus_tag="VFJ72_08235"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7479485.1"
                     /translation="MSDDPRNEADNPGVVAPPPLIYAGALIIGLLVNRLRPIPFLPRT
                     LGRVLGPSLIVGGILIGTFGFREMRRAGTNVDPYKPATTVVQNGPYRYTRNPLYVGMT
                     MIYGGVTARANALPAALLLPAVLHVMQHGVIDREERYLERKFGDEYLNYKSRVRRWI"
     gene            complement(2172..3347)
                     /locus_tag="VFJ72_08240"
     CDS             complement(2172..3347)
                     /locus_tag="VFJ72_08240"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7479486.1"
                     /translation="MERTVILGAARTPFGRFGGALASLSAPELGGAAIREAVDRSGVE
                     DGEIQHSIFGIVVQAGVGQIPSRQANHHAGLPYSLTTETLNQVCASGLRSATLAETMI
                     RAGDYDVILAGGMESMSNAPYMLPKARWGARMGDGAMVDAMMHDGLLDAFEHVNMIRF
                     GTDGAKKFDIPRERQDEWALRSHQRAAAATDEGRLAEEIAGIKVAGKKGQTTYVESDE
                     SIRRDTTLEKLAALKPIDEGGTVTAGNAPGVTDGAGALVLASESFAERRGFEPLGAII
                     AHAKVAEEPPNLLTVPGNAGKLALEKAGWSADDLDLVEINEAFAGVALHSTDMLGVDP
                     DIVNVNGGALALGHPIGASGARILMTLVYELRRRGGSKGLAAICSGGGQGDAVLVEV"
     gene            3486..>3875
                     /locus_tag="VFJ72_08245"
     CDS             3486..>3875
                     /locus_tag="VFJ72_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7479487.1"
                     /translation="MKVKKLDHIALYMADRATAADFLTSHLGFHVVDHTDRYTLIGAG
                     GRIGKLTLFDAPEGTTPSPGVIERINIRVADPEAAAEKLPTEAGAEPRVGGYFFTGPE
                     GLPFALVPGDGEFTDYDLEGFVVRSTEP"
BASE COUNT          640 a         1302 c         1275 g          658 t
ORIGIN      
        1 acctccgggg aggcgccgcc gtaggggagt agttctccgg cttctctcat gtaaatggtt
       61 cctttcttct ccgatcgggc gtcaaccgca gggcgtatcc ggtggtcgaa gtcgtctcta
      121 gccgctgccg ttgatccgcc ataactcgaa cgcccaagcg aggtcgtaga gcacccagat
      181 ccagtacacg gcgacgagcc ccgccgagaa ccgttcatac ctgcgaagcg taaggagcag
      241 cgccgtcagg ggcacgagga acccggctat acccaggaac ccggcgagcg tgctctgcag
      301 atcgaagaac acgtagttcc acaactcgtt caggaacagc acgcagacgg tcagagccag
      361 ggagaccatc ctccgccgcc ggtcgtgaac gtggacgatg acgcggtaga tcacggtgat
      421 aaagagaacg tagtagacgg ccccgacgat gtagaacgcc cacagcggga cgagaaacca
      481 cggtttgtcc agctcgtcgt accagccacc gagggactca ccgacgagag cgccaccgaa
      541 gagggcgaac agcaagcatg tcgctattgc ggccagcaac ggacctatct tcggccaact
      601 cattgggggt tcgctctcac accccctgtg gatgggcttc gaggaagtct aggaggatcc
      661 gccggaactc ctcgggcttc tcgacgtgcg gggagtggcc gcagtcgggg atgacctctt
      721 cacggtagcc tccgccgcgg gcggcgtact cgtccaggac ggcgcgggtc tgggagacca
      781 tcggctgcgg cgggtaggct tccgggccgg gccagccggg gactatctct agctggccga
      841 ggtagccgaa gtccaggagg gaggtgtcgg agacgatctg gtcgtccgag ccccgaaccc
      901 acagcacgtc cgggccgcct tcgagccggg cgaagtcgcc gaggtcgcag tacttaggcg
      961 agatggcgtt gttcatgccc cggctgccgg gcgcgacgga cggccagttc tcggagctcg
     1021 tcatgtcgcc gggatagttg ccttcggtgg tcttggtgga gagcatggag gcgatgtaga
     1081 cctcttcgcg ctcctctctg gcgcggaacg gcggcttgaa gtagaaggcg ttcatgacgt
     1141 tgcgcgggga gttcgggtcc tcgtcggtgc ggtctccctc ggcgagccgc tttacgaact
     1201 ccgggttggc ggtgccgccg ccggagcccg cgtggtccgg ccagcagggt gtcccggcgg
     1261 cgtccttggt gccgccgaaa ccgtagggag acatcggaca gatcagggcg agcgacggga
     1321 cggtctccgg gtggtccatc gcgtactgca tggcgaccgt gccgcccgcc gaccacccga
     1381 caaagtgcgc cctcccgatc cccagggcct cgaccagcgc gtagaggtca tccgcgaagt
     1441 cccgcacgcc gcgcgtggcg tcgagcgact tcgtctcgga gcccccgaaa ccgcggaggt
     1501 ccggtgcgat cccccggtaa gcgtcgggca gcgccacgag cgtctcctcg aagaagagcg
     1561 cggaggagac gttgccgtgg acgaagacga cggggacgcc gccctcgggg ccgctctcca
     1621 gaatatgggt cgccagccgg ggcgtctcga ccatcctagc ggttacgcct tcgagcgtcg
     1681 cgccgaccca aacgttcata tccacctccg cactcgtgat ttgtagttaa ggtactcgtc
     1741 gccgaacttg cgctccaggt agcgttcctc gcggtcgatg acgccgtgct gcatgacgtg
     1801 caagacggcg gggagcaaca gggcggcggg tagcgcgttg gcgcgggcgg tgacgccgcc
     1861 gtagatcatg gtcatgccga cgtagagcgg gttgcgggtg tagcggtacg ggccgttctg
     1921 cacgacggtg gtcgcgggtt tgtacgggtc cacgttcgtc ccggcccggc gcatctcccg
     1981 gaaaccgaat gttccgatca ggataccgcc gactatcagc gacggaccca gcacacgtcc
     2041 gagtgtgcgc ggcaggaatg ggatcggacg caggcggttc actagcaatc cgatgatgag
     2101 ggcgccggcg tagatcaggg gcggcggagc taccacgccg gggttgtccg cctcgtttcg
     2161 cgggtcgtcg ctcatacttc caccagcacc gcgtcgccct gcccaccgcc ggagcagatc
     2221 gccgcgagcc ccttgctccc gccgcggcgg cgcagctcgt acacgagcgt catcaggatt
     2281 cgcgcgccgg atgcgcctat cggatgcccc agcgccagcg cgccgccgtt gacgttcact
     2341 atatcggggt ctacaccgag catgtccgtc gaatgcagcg cgacccccgc gaacgcctcg
     2401 tttatctcca cgaggtccag gtcgtccgcg ctccagccgg ctttctccag cgcgagcttc
     2461 ccggcgttgc ccggcaccgt cagcaggttc ggcggctcct cggcgacctt cgcgtgggcg
     2521 atgatggccc cgagcggttc gaagccgcgg cgttcggcga acgactcgct cgcgagcacg
     2581 agcgccccgg ccccgtcggt gacgccgggc gcgttgccgg cggttacggt gccgccctcg
     2641 tctatgggct tgagggcggc gagcttctcc agcgttgtgt cgcggcggat ggactcatcg
     2701 gattcgacgt aggtggtctg ccccttcttg ccggcgacct tgatcccggc tatctcctcg
     2761 gccagccggc cctcgtccgt ggctgctgcg gcccgttggt ggctcctcag agcccactcg
     2821 tcctggcgtt cgcgagggat atcgaacttc ttcgcgccgt cggtgccgaa ccggatcatg
     2881 ttgacgtgct cgaaggcgtc gaggagcccg tcgtgcatca tggcgtcaac catcgcaccg
     2941 tcacccatgc gcgctcccca gcgggctttc ggtagcatgt aaggggcgtt cgacatgctc
     3001 tccatgccgc cggcgaggat cacgtcgtag tcgccggccc ggatcatggt ctcggcgagc
     3061 gtcgcgctcc tgagccccga ggcgcacacc tggttgagcg tctcggtcgt taaagagtac
     3121 gggagcccgg cgtggtggtt cgcctggcgg gatgggatct ggccgacccc cgcctgcacc
     3181 acgatgccga atatggagtg ctgtatctcc ccgtcctcca cgcccgagcg gtcaaccgcc
     3241 tcccggatcg ccgctccccc aagctccggc gcgctaagag aagccaacgc cccgccgaac
     3301 ctgccgaacg gcgtccgcgc cgccccgagt attaccgtcc gttccaccac tcttcctccg
     3361 aggtcgagac caatggggac agtttaagct ctcgcggcat ggtttgcgtc gcaacctctt
     3421 gccaaaaacg ggctacggcg tgctatcttc ttgacgagtc gaacgtcccg cgaaagggag
     3481 aatccatgaa ggttaagaag ctggatcaca tagccctcta tatggccgac cgcgccaccg
     3541 ccgcggattt cctgacgtca cacctcgggt tccacgtcgt ggaccacacc gaccgctaca
     3601 ccctgatcgg ggcgggaggc cgtatcggta agctcaccct cttcgacgcc ccggagggaa
     3661 ccacaccctc acccggcgtc atcgagcgca taaacatccg cgtcgccgac cccgaagccg
     3721 ccgccgagaa gctgccgacc gaagccggtg ccgagccgcg cgtcggcgga tacttcttca
     3781 ccggccccga agggctcccg ttcgccctgg ttccgggaga cggcgagttc accgactacg
     3841 acctcgaagg cttcgtcgtg cgctccacgg agccg
//
LOCUS       DASSMB010000139        20598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1046325, whole genome shotgun sequence.
ACCESSION   DASSMB010000139 DASSMB010000000
VERSION     DASSMB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 20598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20598
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046325"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..1396)
                     /locus_tag="VFJ72_08250"
     CDS             complement(302..1396)
                     /locus_tag="VFJ72_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7479488.1"
                     /translation="MQPQRDLRDIEEIQELFEAGQETGTLESSEVLDLLQEVDLSTDE
                     IQQVYGLLREHGVEIVDAGFLADTLNEEEDTQIVEEVLESDLRTRYTGDAVQMYLDEI
                     GKTPLLTKAQEVYLAKRIERGDRRAKAHLTRANLRLVVSIAKKYASRGVSLLDLIQEG
                     NIGLMRAVEKFDYRKGYKFSTYATWWIRQAVTRAIADKGRTIRVPVHMVEKINKYYRV
                     QRSLASELNRDPDDEEVARIMEIDAEEVVRIRRVSRRSISLETPVGEDHSSELGDFIA
                     DEESESPHDLAKSSLLKSRMQEALNGLPERERMVLEYRFGLTGGQPKTLEEVGERFDV
                     TRERIRQIQLTALAKIKSSPHAASLRDLLD"
     gene            1509..2267
                     /locus_tag="VFJ72_08255"
     CDS             1509..2267
                     /locus_tag="VFJ72_08255"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HET7479489.1"
                     /translation="MMGLRNLYKVFNESRKAARQQATLAVTGESARADELASLLDAQR
                     SARGAEIILTLSPSGDTLTLSGKGVEDPDEIHLSSVSEQTVLKELIPAVVRALDEEYV
                     VALAKGYPIFRRAACEEVIRKNARQNAMIGALPIPGADMPVMTANQGRMVLNIAAAYG
                     EELSIDRARELLGVLAAGFGLRALSRQVVKLIPVGGWAASAALGYAGTVAMGRATMLY
                     FERGGQQVGQKEMAEIQRRATEEARTFVSRFRKK"
     gene            2324..3604
                     /locus_tag="VFJ72_08260"
     CDS             2324..3604
                     /locus_tag="VFJ72_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HET7479490.1"
                     /translation="MLSSLRRLPSDARAGLADLRKIGVRVFLALTAVQAVLVAALVAL
                     AQLRKRREEPREGFPWAEMPEVQLESGAGTLKLYPYGVDLYEAMISEIEAASENIFLE
                     TFIWKGDELGQRFVDALARKAREGVRVCVIFDGFANMVVPAKFKEFPEEIQTLQFRPI
                     SSPSSVVDPRNLLRDHRKLLTVDGRVAFLGGYNIGQLYARGWRDTHLRIRGGEVREVE
                     NAFIDFWNAHHSESLPRIEPAGERAWNPDLVLHRNDPYLRIFPIRGVYLEAIDRAHER
                     IYMTHAYFVPDRALRAHLIEAARRGVEVQLLLPWESNHITADWLSRRHFHALLEAGVR
                     IFRYKHIMIHSKTATIDGVWSTVGTANIDRFSLLGNYEINLEIYSRRFAEQMERMFEL
                     DKTNAEELTLEEWERRPLLAKCVERILASLSPLV"
     gene            3754..4614
                     /locus_tag="VFJ72_08265"
     CDS             3754..4614
                     /locus_tag="VFJ72_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7479491.1"
                     /translation="MLLAVLFVGNDFVAVKYALEDIPPLVLMPLRFVLAGLVLVSVVG
                     IIERRGRLKRRDLLPVAGVGVVGITLNHLGYTVGLSLTSGSDAALIFATAPVWGMVLG
                     ILLRVERGSLRGALGVGFALAGVGLVVYQGVGSPEASLAGDLLVCVSAFSWGAYAVLS
                     LPLLKRYTPLALAAYTMLLGGAAAIPFALTGLPGISDSVFDVDWSAVGVRSWAGAAYS
                     TFLASAFAIAAWQTSVSRMGANKVLVYLYLVTLVGVTSSVLLLGEGLGAVKIAGAAVI
                     LFGVYVVRRG"
     gene            4695..6353
                     /locus_tag="VFJ72_08270"
     CDS             4695..6353
                     /locus_tag="VFJ72_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET7479492.1"
                     /translation="MARWRLLEPDKDMVSELARSAEVDALCARVLANRGVLPENAEGF
                     LSPSLAAIGMPDEEPWGVAARRVARAIKDGERIGVFGDYDTDGITSAAVLYLALSNYT
                     DNLVVKLPTRQTGYSLLEPYVRDLFAESVSLLVTADCGISNRREVELANSLGMDVIVT
                     DHHIPPEDPPDAAVAVLDPKLWDPDDPLAGVGVAWKFAWAVARELGDADGKRRLGRLL
                     DLVSVGTVVDIAPLRGDNRALASMGLRHINRSLAAGSARPGLAALIKVAGVRGALDEN
                     DLGWKLGPRINSIGRIKDPRPALDLLLTENPREAIRIASMLNQLNSERQRRTRYAVER
                     AMEEVDEEQDFKVVVTEETGGLAGLVAGKVAGATGRPAVVLNRRADGSYGGSARAGET
                     DVDLYGALFAVRHLLGEWGGHRKAAGLSVGAGNFDAFVAGVNEAVRAQADEDPDLFVP
                     AIEVDAEVSLEAVSNGLIEWHERLAPFGSGNYRPVFASTGLRVESSRQLWEGMNLLKL
                     SGGLSAKLSGDPESVPEGDFDAVYTVNRNAYSGGVELEILDWRR"
     gene            6561..7961
                     /locus_tag="VFJ72_08275"
     CDS             6561..7961
                     /locus_tag="VFJ72_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HET7479493.1"
                     /translation="MATGTADAVTRRKFVNTAAAITATGGLLFGYDTGVISGALLFIR
                     KDLGPFSSFTEGVIVSFLLVGAVVGAISGGPLSDRIGRRPTALLAAVVFGIGAIAASL
                     SPSVGFLVFARFLLGLGVGLASMIVPLYIAEIAPADRRGALVSLNQLMITIGILVSYI
                     VGVIFTPIEGWRYMFGVALIPALILGIGMLRLPESPRWLFNVGQVEKARAVLSRSRSP
                     EEVDLEFQEMEQIKRAEEEQEEQVGYRELLSPYVRPALIIGLGLAIFQQITGINTVIY
                     YAPTILQGVGFSEGGAIAATALGVGVVNVGFTILAVRIIDRAGRRLLLIIGLVGMVIS
                     LALLGVVFATGGTGGAAGLLATICLALYIASFAISLGPVFWLMISEIYPLNIRGKAMS
                     VASLANWGSNFLVALTFPVLLATFGGAGSFWLFAVLGIVAWFFVYFWVPETKGKTLEE
                     IEAEFRGVRVASSRVR"
     gene            8013..8969
                     /locus_tag="VFJ72_08280"
     CDS             8013..8969
                     /locus_tag="VFJ72_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7479494.1"
                     /translation="MKAIGVDVGGTKIAAAVVTRNGKILAETRYPSSGPRERLLSSIA
                     RAINDVRDGHDDIGGVCLAVPGFVMSAENKVVFAPNLHTIEGVRLKDEIGQRTDLNIT
                     VENDANAAAWGEFKFGAGSEVDHLVFLTLGTGIGGGVISHGILLRGAQGAGGELGHIT
                     IQAEDGPLCGCGNYGCLEPLASGTSITRRAREVASEHPQSALGQIAVNRNVLGEDVTE
                     LAQQGDELAISVLEETGRWLGVGLAGFVNIFNPDVIAIGGGASRAGELILGPAREEMR
                     RRSQSPSRDLVEIKEATLGPESGVLGAAALARNEKGEYVLGA"
     gene            8977..9780
                     /gene="rsmI"
                     /locus_tag="VFJ72_08285"
     CDS             8977..9780
                     /gene="rsmI"
                     /locus_tag="VFJ72_08285"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET7479495.1"
                     /translation="MPLQVVPTPIGNLEDITLRALRVLREADLIACEDTRRTGRLLAH
                     YGIENRLVAYHEHNEERLAPELAERAKTERVALVTDAGTPLVSDPGYRLIRACIEAGV
                     KVESLPGPSALTTALVASGFPADKVVFLGFPPRKGRERTEVLERVGRESATFVFFESP
                     HRLARTLGDLPEEAPVAVCRELTKLHEEVFRGTAAEAAERYADGAKGEIVLVIRGGTA
                     VETPDFREVLERARGYVAGGESPSRAAARAARESGMKKGEVYGGLVGSD"
     gene            9869..10627
                     /locus_tag="VFJ72_08290"
     CDS             9869..10627
                     /locus_tag="VFJ72_08290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET7479496.1"
                     /translation="MLVDSHTHLLRLKVEPEVAVREAAEAGVVAVVNIGTTIEDSHRG
                     AELAAVLPNVYSSAGIHPHEAGTHTAADLEALAELAESPGIVALGEVGLDYYRGESSG
                     EVQRALFEDAISLANDTRLPLIIHSRQAFADTMASLGNARVPVVLHCFEGGEREVREV
                     RERGYYVGLAGNVTYKNSETARVLRLMDENRLLVETDAPYLSPEPLRGKPNAPKNVVH
                     TARFVAERLGMEDEELAILTARNTRNVFGLPLEL"
     gene            10638..11417
                     /gene="rsmA"
                     /locus_tag="VFJ72_08295"
     CDS             10638..11417
                     /gene="rsmA"
                     /locus_tag="VFJ72_08295"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET7479497.1"
                     /translation="MEPFPKKRLGQHFLKDPNTARIVAEGVTGEDVVLEVGPGRGFLT
                     TFLAKRARLVHAVELDADVLDALRRNVAGYGNVEIHTGDALRFDYASLDPAPNRLVAN
                     LPYNIASPLVLNLLEEVRSLRTLRFMVQLEVARRMAAVRGTKDYAAYAVLVGLLADVR
                     IVHKVSPQVFDPPPRVHSAIVEMERNELDLKSGEYGGVKRLVLAAFRSRRKRLINNLP
                     ESARGGAPRVLGTLGYGPDVRAEEVSSEDFVALYRALSWAI"
     gene            11417..12289
                     /gene="ispE"
                     /locus_tag="VFJ72_08300"
     CDS             11417..12289
                     /gene="ispE"
                     /locus_tag="VFJ72_08300"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET7479498.1"
                     /translation="MRGIHLRAFAKVNYALDVLGVREDGYHEISTVMQSVSLADEVEI
                     TRAEGGLDLRVEPEGVEVGPLERNTIYRAWTLLRERTGRGLPVRVRVLKKIPAGAGLG
                     GGSADAAAALIGLNRLFDLGLEVEDLAELGVRIGADVPFCLTGGTALGEGVGEVLTAL
                     PAPPQHFLVIAKPAASADTGEVYRAYDELPPCGSGISDALALALRSGDLRVFAEAAGN
                     DLARVTRELVPEVENLERALLQAGALGVSMTGTGTAVYGIFANWEDALAAADRIETSF
                     ASVFEPVSTGVEEI"
     gene            12402..12472
                     /locus_tag="VFJ72_08305"
     tRNA            12402..12472
                     /locus_tag="VFJ72_08305"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12434..12436,aa:Gln,seq:ttg)
     gene            12519..13934
                     /gene="glmU"
                     /locus_tag="VFJ72_08310"
     CDS             12519..13934
                     /gene="glmU"
                     /locus_tag="VFJ72_08310"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7479499.1"
                     /translation="MSEHGTPSEIFAVVLAGGKGTRMKSNRAKVLHTLCGVPMVNYAI
                     EAIRPLVPESLSVVVGHQADLVEAVLPPDARPVFQKEQLGTGDAVRVALEAIEEEEGI
                     LLVVNGDGPLISAGTLGELVERHRGAGVGATVLVAELPDPSGLGRVAEDAGVVRIVEE
                     REATETERRINLVNLGLYAFDLAEIRDALGRLESNNSQGELYLTDALEIIGRRSRAVT
                     YRLKNLEEANLVNDRAHLARAEEIIRRRILDAHMRAGVTVRDPASTHIEASVEIGRDT
                     IIFPGTFLRGETSIGSDCVIGPSADLLDTTVGDGALVEHSVGRGAEIGAGANVGPYAY
                     LRPGTVLGRKAKVGAYCELKNTKVGDGSKVPHLSYVGDAEIGEGANLGAGTITANYDG
                     KNKNRTVIGDGAFTGVNTNLIAPVTIGDGAYLGAGSVVTRDIPPGKLAFGMPARVIRD
                     SPNKIKNKIEDDRRSNGEMEA"
     gene            13942..14934
                     /locus_tag="VFJ72_08315"
     CDS             13942..14934
                     /locus_tag="VFJ72_08315"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7479500.1"
                     /translation="MQNGYLTHTTEKRLMLFSGDGYPQLAERIADRLDLDLGNVDKQR
                     FPSGEMYARYMESVRGADVFIVQSLGEPVNRNLMQLMVMIDAAKRASAKRITAVLPYY
                     AYSRQDRKTKPREPITARLVANMIQTAGAERVMTMDLHQGQVEGFFAFPVDHLTAMHT
                     FVDYFVEAGFKDADDVVVVAPDTGEAKVANRLADHLGVPWAILNKRRSGPGQSEVTHV
                     IGDVEGRRAIMIDDLIDTAGTLCNAAEILIRKGATEVYAAGTHAIFSGPAYRRIEDSS
                     IKEVVVTDTLPLKEGEPRSKIRTLTIAPILASTIKNVFLDDSVSEVFMGENQLF"
     gene            14962..15678
                     /locus_tag="VFJ72_08320"
     CDS             14962..15678
                     /locus_tag="VFJ72_08320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET7479501.1"
                     /translation="MANNVDLQAKKREGRGKNDSRRLRREGMAPAVLYGDEGSTAIVV
                     PARTVDYTLQHVGDNALYDIDLGDGAATARVVDVQRNPVSGILTHVDFAPVNMRERIE
                     VTVPLHIVGEAPGVEEGGVLQQVAYEIQVESLPGDIPQEVTLDVSSLGMNENLTLGDL
                     TLPEGVDLLSEPEDVAVTITQPTEISEEEMEAAGIVEEPTDEEEAAAAEEAAEGEEAA
                     EGEEASADEGEEPEGQDEQA"
     gene            15644..16273
                     /gene="pth"
                     /locus_tag="VFJ72_08325"
     CDS             15644..16273
                     /gene="pth"
                     /locus_tag="VFJ72_08325"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7479502.1"
                     /translation="MRNPRARTSRLNELRQSFLDRRNRQEESGGPRRSPVVVGLGNPG
                     RSYERTRHNAGYLVADELAKRHNGSWRKKKKSEAAPISLGLKNVTLLKPTTYMNNSGS
                     ALSDSRVEDLIVVHDDLDLEEGTVRVKVGGGAGGHNGLRSIIQNKGNDFTRVRIGIGR
                     PPIGVTVTDYVLGRMDSAVKDAIPTAADVVEFLVENGAEAAMNRFNVRS"
     gene            16577..17548
                     /locus_tag="VFJ72_08330"
     CDS             16577..17548
                     /locus_tag="VFJ72_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET7479503.1"
                     /translation="MKAIVRYKYGSPDVLELQDIDKPQISDDEVLVHVHAAGVDRGVW
                     HLMTGLAYPIRLAGYGLRAPKNPVLGGDLAGVVVAIGKSVTRFRPGDEVFGIGEGSYA
                     EYARALEKKLAPKPANLTFEQAAVVAISGLTALQAVRDHGKARPGQKVLIIGASGGVG
                     TYAVQIARVFGARVTGVCSTAKVEMVRSIGADHVIDYTKEDFAEGGQRYDLILDIGGN
                     SPLARLRRALTPKGTLVIVGGESDGRWLGGTDRQLRAMMLSPFVSQKLLAFINKENHE
                     DMLVLKELIEAGKVTPVLDRTYPLAEAPDAIRYLTEGHVRGKVVITI"
     gene            17608..18372
                     /locus_tag="VFJ72_08335"
     CDS             17608..18372
                     /locus_tag="VFJ72_08335"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HET7479504.1"
                     /translation="MDVHPLTSSTPRTTRKGQQGTAHTTADVSLRGAAAVAGLGLLVM
                     AVLAFASFAVFGNLVATGDAAKTARNIVDHELLFRATICGFLIVAVLDVVVAWALYVL
                     LRPAGRSIALLAAWFRVVYAALFAAALSNLFVAARLPTDADSLESFGAGRLNAQVLMY
                     VNAFDDGYHAALAIFGLHLLVLGYLVFKSGYIPRVLGILVMIASFGYLIDGFGGSLSA
                     GYNANLIQFTFIGEVLLMVWLLWKGVRLKNHPQREI"
     gene            18809..19231
                     /locus_tag="VFJ72_08340"
     CDS             18809..19231
                     /locus_tag="VFJ72_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479505.1"
                     /translation="MADSRRLAGLIGPTMLVLGVSEAVNFRIWETNVAPLIYLNGLLL
                     FVAGLSIVRVHNLWMRGWPVMVTLVGWVAVLGGLFRMFAPEVQQAGQNAPTTIITASL
                     VGAIGLFLTFKAYGPVARSKPTGSGQDGGRQLADRGQA"
     gene            19391..19948
                     /locus_tag="VFJ72_08345"
     CDS             19391..19948
                     /locus_tag="VFJ72_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET7479506.1"
                     /translation="MTTRTFSWLKDHPLVVFLLGVALAYNVLMNNTAFRRWLYRGRRP
                     NRIAKVMNRAAAAMASSGVASGPMVTLEVRGRKSGRTISLPLVMASVDGQRYLVSMLG
                     DDVQWVRNVRATGGRAVIRTRDREAVRLEEVAAEQRAPILKAYLKRAPGARWHFPIDK
                     EAPLSEFERVAPRFPVFRVVPGNAA"
     gene            20286..>20598
                     /locus_tag="VFJ72_08350"
     CDS             20286..>20598
                     /locus_tag="VFJ72_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7479507.1"
                     /translation="MSTIKEAASEFLARRRVAVTGVSREPQSHGSNVVYRRFRERGYE
                     VFAVNPNADEVEGDACYHDLRSIPGGVEAVVIGTRPEVAEETMRECAELVLCHSLDAR
                     FE"
BASE COUNT         3654 a         6170 c         7074 g         3700 t
ORIGIN      
        1 ggtatcggcg ccacttttca agagttatac gaggggacag gttccctgcg cacggaactt
       61 gtcgagtacg agcggtccag gcggctctcg tttcgtagcg acggcccgcg catgcgcatc
      121 gcaggcacgt tcatcctcgc atccgttcag aaagggagcg acgtggcgct cgatgccgac
      181 ctgaagccgg caaggccctt tcaggcttat cgcttcgctg atggccccca cgatcaaacg
      241 gcggaacgcc gccgcagtca gattgaaacg agtgctggag tcggcctagg ccgacacgga
      301 tctagtccag caggtcgcgc agggaagccg cgtgtgggct gctcttgatc ttggcgaggg
      361 cggtcagttg gatctgacgg atgcgctcgc gggtaacgtc gaagcgctcg ccgacctctt
      421 cgagcgtctt cggctggccg ccggtaagac cgaagcggta ctccagcacc atccgctcgc
      481 gttccgggag gccgtttaag gcctcttgca tccgggactt caggagtgag gacttggcga
      541 ggtcgtgcgg agactctgac tcctcgtcgg cgatgaagtc gccgagttcg gaggagtggt
      601 cctcgccgac cggtgtctcc agggagatag agcggcggga gacgcgccgg atgcggacga
      661 cctcttccgc gtctatctcc atgatgcgcg cgacctcctc gtcgtcgggg tcgcggttca
      721 gctcggaggc gagtgagcgt tggacgcggt agtacttatt gatcttctcg accatgtgga
      781 ccgggacgcg gatcgtgcgg cccttgtcgg ctatggcgcg ggtgacggcc tgacggatcc
      841 accacgtcgc gtaggtggag aacttgtatc ccttgcggta gtcgaacttc tcgaccgccc
      901 gcatcagccc gatgttgccc tcctggatca gatccagaag agagacgccg cggctggcgt
      961 acttcttggc gatggagacg acgagcctga ggttcgctcg cgtgaggtgg gccttcgccc
     1021 gccggtcgcc gcgctcgatc cgcttggcaa gatagacctc ctgggctttg gtgaggagag
     1081 gggtcttgcc gatctcatcc agatacatct ggacggcgtc gccggtgtag cgagtacgca
     1141 agtcactctc cagtacctct tcgacgatct gggtatcttc ttcttcgttc aaagtgtcgg
     1201 cgaggaaccc ggcgtccacg atctcgacgc cgtgttcgcg gaggaggccg tagacctgtt
     1261 ggatctcgtc ggtagagagg tctacctctt gcagaaggtc gagtacctcg ctggattcga
     1321 gggtgccggt ctcctggccg gcctcgaaga gttcctggat ttcctctata tcccgcaggt
     1381 cgcgctgagg ttgcaagttc tcctctagct ctctagcctg aaactctcct tctacctaag
     1441 cgtaatatat ccgaaaaagg gcaaagatca attgaagtca gcatatgtat cgggaggagc
     1501 agtaagccat gatgggtctt aggaatctct acaaggtgtt taacgagtct cgcaaggccg
     1561 cgcgccagca ggcgacgctg gccgttaccg gcgaatccgc gcgcgccgac gaactggcaa
     1621 gcctcctcga cgcgcaacgg agcgcgcggg gagcggagat catcctgacc ctctcgccct
     1681 ccggcgacac gctgacgctc tccggcaagg gagtcgaaga cccggatgag atccatctct
     1741 cctcggtgag cgagcagacc gttctgaaag agttgatccc cgccgtggtc cgggctctgg
     1801 atgaggagta cgtggtggcg ctggcgaagg ggtatcctat ctttcgccgg gccgcctgtg
     1861 aggaagtgat ccgcaagaac gcccgtcaga atgccatgat cggggcgctc cccatccccg
     1921 gcgcggacat gccggtaatg accgccaacc agggccggat ggtcctgaac atcgccgccg
     1981 cctacggtga ggaactgtcc atagaccgcg cccgcgagct tctcggggtg ctggctgcgg
     2041 gcttcgggct gcgggccctg agccgtcagg tggtgaagct gatccccgtc ggcggctggg
     2101 ctgcgtcggc ggctctcggc tacgccggga cggtagcgat gggccgtgcc acgatgctct
     2161 acttcgagcg cggcgggcag caggtaggcc agaaagagat ggccgagata caacgccgcg
     2221 ccaccgagga ggcccgaacc ttcgtctcac ggttccgcaa gaagtagtgc cgcccggacg
     2281 ccgcgcggca tcgcggtccg gcgcgttgaa tataatcggc ggcatgctct cttctctccg
     2341 gcggttgcca tcggatgcgc gggccggtct cgccgacctg cggaagatcg gggtcagggt
     2401 ctttcttgcc ctgaccgccg tgcaggcggt gctcgtcgcg gcgctggtgg cgctggctca
     2461 acttcgcaag cgccgggagg agccgcgcga gggcttcccg tgggctgaga tgcccgaggt
     2521 ccagctagag agcggcgctg gaaccctcaa gctctatccg tatggcgtgg acctctatga
     2581 ggccatgatc tccgagatag aagccgccag tgagaatatc ttcctggaga cctttatctg
     2641 gaagggcgac gagctgggcc agcgtttcgt ggacgccctg gcccgcaagg cccgcgaggg
     2701 cgtgcgggtg tgcgtgatct tcgatggctt cgccaacatg gtcgtgccag cgaagttcaa
     2761 ggagttcccg gaggagatac agaccctcca gttccgcccg atctccagcc cgtcgagcgt
     2821 cgtggacccg cgcaacctgc tccgcgacca ccgcaagctg ctgaccgtgg acggccgggt
     2881 ggcgttcctc ggcggctaca acatcggcca gctctacgcg aggggctggc gcgacacgca
     2941 cctgaggatc aggggcggcg aggtccggga ggtcgagaac gccttcatag acttctggaa
     3001 cgcgcaccac agcgagtctt tgccgaggat cgaacccgcc ggagaacgcg cctggaaccc
     3061 ggacctcgtg ctgcaccgca acgacccgta tcttcgcatc ttcccgatcc ggggcgtcta
     3121 cctggaggcg atagaccggg cgcacgagcg catctatatg acccacgcct acttcgtccc
     3181 cgaccgggcg ctccgggcgc acctgatcga ggcggcgcga aggggtgtgg aggtgcagtt
     3241 gctcctcccc tgggaatcga accacatcac tgccgactgg ctctcgcggc gccacttcca
     3301 cgcgctgctt gaggcggggg tcaggatctt ccgctacaaa cacatcatga tccactccaa
     3361 gaccgccacc attgacggcg tctggtccac cgtcggcacc gcgaacatag accgcttcag
     3421 cctgctcggc aactacgaga taaacctgga gatctacagc cgccgcttcg cggagcagat
     3481 ggagcggatg ttcgagctgg acaagaccaa cgccgaggag ctcaccctcg aagagtggga
     3541 gcgtcgcccg ctcctcgcca agtgcgtcga gcgcattctc gcttccttga gcccgctggt
     3601 ctgatcgccc accaaggtct ttcggcgagg agatatttcg tatggggctt tgccctagaa
     3661 tgcctttgca tgaacggatc acggctggag agagacagaa gcttcacgga cggcgcgcaa
     3721 ggaggcggga cttccgcgct ctcagaggca tccttgcttc tcgcggttct cttcgtcggc
     3781 aacgatttcg tcgcggtcaa gtacgctctg gaagacatcc ccccgctggt cctgatgccg
     3841 ctacgctttg tgttggcggg tctcgtactg gtgagtgtgg tggggataat cgagcgaaga
     3901 ggtcgcctga agcggaggga tctcttgccg gtcgccgggg tcggcgtcgt gggcataacg
     3961 ctgaaccacc tcgggtatac ggtgggcttg agcctcacga gcggctcgga cgccgccctg
     4021 atcttcgcca cggcgcctgt ctggggcatg gtgctgggaa tcctgttgcg ggtcgaacgg
     4081 ggatctcttc gcggggctct cggtgtcggg ttcgctctcg ctggagtagg actggtcgtt
     4141 tatcaaggcg tgggttctcc agaagcctca cttgcgggcg atttgctggt gtgcgtcagc
     4201 gcattttcgt ggggagcata cgcggtgctc tcccttcctt tgctgaagcg ctacacgccg
     4261 ctcgcgctcg ctgcctacac tatgttgctc gggggagccg ccgcgattcc tttcgctctg
     4321 actgggcttc caggtatctc cgactcggtg ttcgatgttg attggagcgc tgtgggagtg
     4381 cggtcctggg ctggagcggc atattccacg ttcctggcga gcgcgttcgc aatagccgcc
     4441 tggcagacaa gcgtttcccg gatgggagcg aacaaggtgc tggtatacct gtacctggtg
     4501 acgctcgtag gtgtaacctc cagcgtgttg ctcctggggg aaggactagg ggcggtaaaa
     4561 attgcggggg cagccgtgat cctcttcgga gtatacgtgg tccggcgcgg ctgaacgacc
     4621 gaaagcgccg atcctctctg tataatatct atgcgtagct cttgactttt cgcggggacg
     4681 acgggaggac ctgattggcg cgctggcggc ttcttgagcc ggacaaggac atggtatccg
     4741 aacttgcgcg gtccgcggag gtggatgcgc tctgcgcccg tgtccttgcg aaccggggag
     4801 tcctgcccga gaacgcggaa ggcttcctct cgccctcgct cgcggccatc gggatgccgg
     4861 atgaggagcc gtggggtgtg gccgcgcgaa gggtggcgcg ggccatcaag gatggggaga
     4921 ggatcggggt cttcggcgac tacgataccg acggcattac ctccgccgcc gtgctctacc
     4981 tggcgctctc gaactacacg gacaacctgg tcgtaaagct cccgacccgg cagaccggct
     5041 acagtctgct ggagccttac gtgcgagacc tcttcgccga gagcgtgagc ttgctcgtga
     5101 cggcggactg cggtatctcc aaccgccgcg aggtcgagct tgcgaattct ctcggcatgg
     5161 acgtgatcgt gaccgaccat cacatcccgc ccgaggaccc tccggacgcc gccgttgccg
     5221 tcctcgaccc gaagctctgg gacccggacg atccgctcgc cggggtcggg gtcgcctgga
     5281 agttcgcctg ggccgtcgcc cgcgagctgg gagacgctga cggcaagcgc cgccttggac
     5341 ggctcctgga cctggtctca gtcgggaccg tggtggacat cgcgccgctc aggggagaca
     5401 accgggcgct ggcctcgatg ggcctgagac acatcaaccg ttcgctcgcc gctggtagcg
     5461 cgcggccggg actcgcggcg ctaatcaagg tggcgggggt gcggggcgcc ctggacgaga
     5521 acgacctggg ctggaagctc ggcccgcgca tcaactccat cggcaggatc aaagaccccc
     5581 gcccggcgct ggacctgctc ttgacggaaa atccgcggga ggccatacgc atagcctcca
     5641 tgctcaacca gctcaactcc gagcgtcagc ggcgcacccg ctacgccgtg gagcgggcga
     5701 tggaggaggt ggacgaggag caggacttca aggtagtcgt gacggaggag acgggcgggc
     5761 tggcgggcct cgtcgccggc aaggtagccg gggctacggg gcgcccggcg gtggtcctca
     5821 accggcgcgc cgacggctcc tacggcgggt cggcacgggc tggcgagacg gatgtggacc
     5881 tctatggcgc gctcttcgcg gtgcggcatc tgctgggaga gtggggcggg caccgcaagg
     5941 cggcgggact cagtgtcggg gcgggcaact tcgacgcttt cgtagccggg gtcaacgagg
     6001 cggtgcgagc gcaggcggac gaggacccgg acctcttcgt cccggctata gaggtggacg
     6061 cggaggtgtc gctggaggcg gtctccaatg gcctgatcga atggcacgag aggctcgcgc
     6121 cattcgggtc cggcaactac cggccggtct tcgccagcac ggggctgcgg gtggagagct
     6181 cgcgccagct ctgggaaggg atgaacctac tcaagctctc cggcggtctc tccgccaagc
     6241 tctccggaga cccggaatcg gtcccggaag gtgacttcga tgctgtctat accgttaacc
     6301 gcaatgctta ctcgggagga gtggagctag agatactgga ctggagaagg taggacttcg
     6361 ctcgcctgca agatccacgg ctctgtattt tgcgaggtgt gatgaccgta gcgcgaggcg
     6421 ggtgatctgc gttgacccgt tcttgcccgt tcttgttgga cattccggta gaagggcctg
     6481 aggaatgccc gagttggttg tcgccgttct tgggcatata ctgctactgg ggttacgcgg
     6541 aaaagggaag gggtggagag atggctacgg gcactgcgga tgcggttact cggcggaagt
     6601 tcgtcaacac tgccgcggcg atcacggcga cgggaggatt gctattcggc tacgacacgg
     6661 gcgtgatctc cggcgccctt ctctttatcc gaaaggacct gggacctttc tcatccttca
     6721 ccgaaggtgt gatcgtcagc ttcttgctcg tcggcgcggt ggtcggggcg atctcgggcg
     6781 ggccgctctc cgaccggatc ggacgaaggc ctaccgcgct tctcgccgcc gtcgttttcg
     6841 gcattggggc cattgccgcg tctctctcgc cgagtgtggg gttcctggtc ttcgcgcggt
     6901 tcctgctggg gttaggcgtg ggactggctt cgatgatagt accgctctac atcgccgaga
     6961 tcgccccggc ggacaggcgt ggcgcgttgg tatcgctgaa ccagctcatg atcaccatag
     7021 gcatccttgt ctcctacata gtcggcgtca tctttactcc catcgagggc tggcgctaca
     7081 tgttcggggt cgcgctcata ccggccctca tcctcggcat tgggatgctc cggttgcccg
     7141 agagcccgcg ctggctcttc aatgtgggcc aggtagagaa ggcccgcgcc gtgctcagcc
     7201 gctcccgaag cccggaggaa gtagatctgg agtttcagga gatggagcag ataaagcgcg
     7261 ccgaggagga gcaggaggag caagtcggct acagggagct cctgtcacct tacgtgaggc
     7321 ccgccctgat catcggcctc gggctcgcta tcttccagca gataaccggc atcaacaccg
     7381 tcatctacta cgctcccacg atcctccagg gagtcggctt ctccgaaggc ggggccattg
     7441 ccgccaccgc cctcggcgtg ggtgtggtaa acgtcgggtt tacgatcctc gccgtccgga
     7501 tcatagaccg tgcgggacgg cggctgctgc tcatcatcgg cctcgtcggc atggtcatca
     7561 gcctggctct ccttggcgtg gtctttgcga ccggaggcac cggaggcgcg gcggggctgc
     7621 tggcgaccat ttgtctcgcg ctgtacatag cctcgtttgc gataagcctc ggtccggtgt
     7681 tctggctcat gatctcggag atctaccctc tgaacatccg cggcaaggcc atgagcgtgg
     7741 cgtccctggc gaactggggg tcgaacttcc tcgtcgcgct gaccttcccc gtattgctcg
     7801 cgacgttcgg aggggctggc tcgttctggc tgttcgcggt gctcgggatc gtggcgtggt
     7861 tcttcgtcta cttctgggtg ccggagacca agggaaagac tctggaagag atagaagcgg
     7921 agttccgcgg cgtgcgagtc gccagcagcc gggtgcgctg aagggaggtt gatggaggtg
     7981 atgacaaaat ctcacggcag gaggacggct tgatgaaagc cataggcgtt gacgtcgggg
     8041 ggaccaagat cgccgcggcc gtcgtgacgc ggaacggcaa gatcctggcc gagacccgct
     8101 acccttcttc ggggcctcgc gagcggctgc tctccagcat agcccgcgcc attaacgacg
     8161 tgcgcgacgg acacgacgat ataggcggcg tgtgtctcgc ggtgcccggt ttcgtgatgt
     8221 cggcggagaa taaggttgtc ttcgcgccca acctgcacac catcgagggc gtgcggctca
     8281 aggacgagat cggccagagg acggacctca atattaccgt cgagaacgac gccaacgctg
     8341 cggcgtgggg cgagttcaag ttcggggcgg gcagcgaggt ggaccatctc gtgttcttga
     8401 cgcttgggac cgggatcggg ggaggcgtga tctcgcacgg catcctcctg cgcggcgcgc
     8461 agggtgcggg cggcgagctg ggacacatta ccatccaggc cgaggacggt cccctgtgcg
     8521 gctgcggcaa ctacggatgc ctggagcctc ttgcctccgg tacctccata acccgccgcg
     8581 cgcgggaggt cgccagcgag cacccgcaat ccgcgctggg ccagatcgcc gttaaccgca
     8641 acgtcctggg cgaggacgta acggagcttg cgcaacaggg cgacgagttg gctatctcgg
     8701 tattggagga gaccggcagg tggctgggag tagggctggc gggcttcgtc aacattttta
     8761 accccgatgt catagctatc ggcggcgggg cgtcgagagc gggagagctg atcctgggtc
     8821 ccgcccgcga ggagatgcgt cggcgctccc agagccccag ccgcgacctc gtggagatca
     8881 aggaagccac cctgggcccg gaatccggcg tgctcggagc cgccgcactc gcgcgcaacg
     8941 aaaagggcga gtacgtcctg ggggcataga gacctcttgc ccctccaggt cgttccgacc
     9001 cccataggga acctcgaaga tataaccctg cgtgcgctgc gagtcctgcg cgaggccgac
     9061 ctcatagcct gcgaggacac tcgccgcacc gggcgtttgc tcgcccacta cggtatcgag
     9121 aacaggctcg tcgcctacca cgagcataac gaggagcgtc tggctcccga gctcgccgaa
     9181 cgcgcgaaga cggagcgggt cgcgctcgtc accgacgccg ggacgccgct cgtctccgac
     9241 cccggatacc ggctgatccg ggcctgtata gaggccgggg tgaaggtcga gtcgctaccc
     9301 ggaccctctg ccctgactac cgctctcgtc gcctctggtt tcccggcgga taaggtcgta
     9361 ttcctgggat tcccaccgcg caagggtcgc gagcggacgg aagtcctgga gcgggtcggc
     9421 cgcgagagcg cgaccttcgt gtttttcgag tcgccgcacc ggctggcgag gacgctcgga
     9481 gatctcccgg aggaggcgcc ggtcgcggtc tgccgggagc tgacgaagct gcacgaggag
     9541 gtcttccgcg gaacggcggc ggaggcggcg gagcgctacg cggatggtgc gaagggagag
     9601 atcgtactgg taatacgcgg cgggactgct gtcgagacgc ctgacttcag ggaagtcctg
     9661 gagcgggcgc gcggatatgt ggcgggaggt gagagcccgt ccagggcggc ggcgcgggcc
     9721 gcgcgggagt ccgggatgaa gaagggcgag gtatacgggg ggctcgtcgg ttccgattag
     9781 cggttccggt cgggcgccgg atgacgccgg gtttcaggcc gctctataat tctgcccgtg
     9841 tccgtcgtgg ggagaggggt tggactttat gctcgtggat tcgcatacgc acctgttgcg
     9901 gctgaaggtg gagccggagg tggccgtgag agaggcggcc gaggcggggg tcgtggcggt
     9961 ggtgaatatc gggacgacca tcgaggactc gcaccggggg gcggagcttg cggcggtcct
    10021 gcccaacgtc tactcctcgg ccgggattca cccgcacgag gccggcacgc acaccgccgc
    10081 ggacctggag gcgctcgcgg agcttgcgga gtcgccgggg atcgtggctc tcggcgaggt
    10141 cgggctcgat tattaccggg gggaatcgtc gggggaggtg cagcgggcgc tgttcgagga
    10201 tgccatatcg ctcgccaacg acacgcggct gcccctgatc atccactccc gccaggcttt
    10261 tgccgacacg atggcctccc tcggcaacgc tcgcgtcccc gtcgtcctcc actgcttcga
    10321 gggcggcgaa cgtgaggtgc gggaggtccg cgaacgcggt tactacgtgg ggctcgcggg
    10381 caacgtgacc tacaagaaca gcgagacggc gcgggtgctg cgcctgatgg acgagaaccg
    10441 tctgctggtc gagaccgacg cgccctacct cagccccgaa cccttgcgcg gcaagccgaa
    10501 cgcgccgaag aacgtggtcc acacggcgcg cttcgtcgcc gagcgactgg ggatggagga
    10561 tgaggaactg gcgatcctca ccgcccgcaa cacccgcaac gtcttcgggc tgccgctgga
    10621 gctctgagac gctcttcgtg gagcctttcc ccaagaagcg cctcggccag cacttcctga
    10681 aggacccgaa caccgcccgc atcgtggccg agggcgtcac gggagaggac gtggtcctcg
    10741 aagtcgggcc ggggcgcggg ttcctgacga cgttcctcgc caagcgggcg cggctcgtgc
    10801 acgcggtgga gctggacgcg gatgtcctcg acgccctgcg ccgcaacgtc gccggatacg
    10861 gcaacgtcga gatccatacg ggcgatgccc tgcgcttcga ctacgcgagc ctcgaccccg
    10921 ccccgaacag gctcgtcgcc aacctcccct acaacatcgc gtcgccgctg gtcctgaact
    10981 tgctggagga ggttcgcagc ctgagaaccc tgcgtttcat ggtgcagcta gaggtggcgc
    11041 gccggatggc cgccgtccga gggaccaagg attacgcggc gtatgccgtc ctggtgggct
    11101 tgctcgcgga cgtgcgaatc gttcacaaag tctcgccgca ggtcttcgac ccgccgccgc
    11161 gggtacactc tgctatcgtg gagatggaac gaaacgagtt ggaccttaaa tcaggcgagt
    11221 acggcggcgt gaagcgtctc gtgctcgcgg ccttccggag ccggcgcaag cgtctgatca
    11281 acaacctgcc cgagtcggcg cgggggggag cgccgagagt tctgggaacc ctcggatacg
    11341 ggccggacgt cagggcggag gaagtatcct ccgaggattt cgtggcgctc taccgggcgc
    11401 tctcgtgggc gatctgatgc gtgggatcca cctgcgcgcg ttcgccaagg tcaactacgc
    11461 tctggacgtc ctcggcgtcc gggaggacgg ttaccacgag atctcgaccg tcatgcagag
    11521 cgtctcgctg gccgacgagg tcgagataac tcgcgccgaa ggcggcttgg atctgcgcgt
    11581 tgagcccgag ggcgtcgagg tcgggccgct ggagcggaac acgatctaca gggcgtggac
    11641 cctgctgcgc gaacggacgg gccggggtct cccggtccgg gtccgggtct tgaagaagat
    11701 accggcgggc gcggggcttg gcgggggctc ggccgacgcc gccgccgccc tgatcgggct
    11761 gaatagactc tttgatctgg ggctagaggt tgaagacctg gcggagctcg gggtacgcat
    11821 cggcgcggac gtgccgttct gcctgaccgg cggcacggct cttggagagg gagtcggaga
    11881 ggtgttgacc gctctacccg cgccgccgca gcactttctc gtcatcgcca aacctgccgc
    11941 gagcgcagat accggagagg tataccgggc ttatgacgag cttcctccat gcggctcggg
    12001 catctcggac gcgctggcgc tggcgctgcg ttcgggagac ttgcgggtat tcgccgaggc
    12061 tgccgggaac gaccttgctc gggttacccg agagttggtg ccagaggtgg agaatctcga
    12121 acgggcgtta ttgcaggctg gcgcactcgg cgtctcgatg accgggactg gtacggctgt
    12181 ctacggcatc tttgccaatt gggaggacgc gctagcagcc gcggaccgga tcgagacctc
    12241 gttcgcgagc gtgttcgagc cggtatctac cggagtagag gagatttagg ggcccgaaca
    12301 tttctggtcc gcgaaccgga tgatagctct gcggggctcc ggtataatcg cggcgtcgcg
    12361 ggtactatga aagagcgccg gccgtctcga aacgagaccg atggggcgta gccaagtggc
    12421 aaggcaccgg gttttggtcc cggcatcgca ggttcgaatc ctgccgcccc agctatcagg
    12481 ccgcaggtta gccagactca aaggagcctc agagaagtat gtccgagcat ggaacgccgt
    12541 cagagatctt cgccgtggtc ctcgccggag gtaagggcac ccggatgaag tccaatcggg
    12601 cgaaggtgct ccataccctc tgcggcgtac ctatggtgaa ttacgctatc gaggcgatcc
    12661 gtcctctggt ccccgagagc ctctccgtcg tggtgggcca tcaggcggat ctggtcgagg
    12721 cggtgctgcc gccggacgcg cggcctgtct tccagaagga gcaactgggg accggcgacg
    12781 ccgtccgcgt ggctctggaa gctatcgaag aggaagaagg catcctgctg gtggtgaacg
    12841 gcgatggtcc gctcatctcg gccgggacgc tgggcgagct ggtcgagcgg caccggggcg
    12901 cgggcgtggg cgcgactgtg ttagtcgcgg agctgccgga cccgagcggg ctgggccgcg
    12961 ttgcggagga cgcgggagtg gtgaggatcg tggaggagcg tgaggcgacg gagaccgagc
    13021 gccggataaa tctcgtcaac ctgggacttt atgcgttcga cctcgcggag atccgggacg
    13081 ccctggggcg gctggagtcg aacaacagcc agggcgagct gtacctgacg gacgccctgg
    13141 agatcatcgg ccgccgcagc cgcgccgtta cctaccgcct gaagaacctg gaagaggcga
    13201 acctggtgaa cgaccgggcg cacctggcgc gggcggagga gatcatccgc cgccgcatcc
    13261 tggacgctca tatgcgcgcc ggcgtcaccg ttcgggaccc ggcctccacg cacatcgagg
    13321 cgagcgtgga gatcggccgc gacaccataa tcttccccgg cacgttcctg cgcggagaaa
    13381 cgagtatcgg ctcggactgc gtcatcggcc cgtccgccga cctgctggat acgaccgtcg
    13441 gcgacggcgc cctggtcgag cactcggtcg ggcgcggggc ggagatcggt gctggcgcga
    13501 acgttgggcc ttacgcttac ctgcggccgg gcacggtgct ggggaggaag gcgaaggtcg
    13561 gggcgtactg cgagctcaag aacacgaagg tcggcgacgg cagcaaggtg ccgcatctct
    13621 catatgtcgg ggatgcggag ataggggagg gggcgaacct gggggccggg acgatcacgg
    13681 cgaactacga cggcaagaac aagaaccgga cggtcatcgg ggatggcgct ttcacggggg
    13741 tgaacactaa tttgatagct ccggttacaa taggagacgg cgcctacctg ggggccggaa
    13801 gcgtggtcac cagagacatc ccgccgggca agctcgcgtt cgggatgccg gcgcgtgtta
    13861 tccgggactc tccgaataaa ataaagaata agatagaaga cgaccggcgt tcaaacggcg
    13921 agatggaggc ttagcgcggt tatgcagaac ggttacctga cgcacacgac cgagaagcgc
    13981 ctgatgctgt tctcgggcga cggctacccg caactcgccg agcgcatagc ggaccggctg
    14041 gacctggatc tcgggaacgt ggacaagcag aggtttccaa gcggggagat gtacgcccgg
    14101 tacatggaga gcgtccgggg ggcggacgtg ttcatcgtcc agtccctcgg agagcccgtg
    14161 aaccgcaacc tgatgcagct aatggtcatg atcgacgccg ccaagcgcgc ctcggccaag
    14221 aggataacgg cggtgctgcc gtactacgcc tactcgcgcc aggacaggaa gaccaagccg
    14281 cgcgagccca tcactgcgcg gctggtggcg aacatgatcc agaccgccgg cgccgagcgg
    14341 gtgatgacga tggaccttca ccaggggcag gtggagggct tcttcgcctt cccggtggat
    14401 cacctgaccg cgatgcacac gttcgtggac tacttcgtgg aggcgggctt caaggacgcc
    14461 gacgacgtgg tggtagtcgc cccggacacg ggagaggcga aggtggcgaa ccgcctggcg
    14521 gaccacctcg gggtgccgtg ggcgatcctc aacaagcggc gcagcggtcc gggccagtcc
    14581 gaagtgacgc acgtcatcgg cgacgtggag gggcggcgcg cgatcatgat agacgacctg
    14641 atagacaccg caggcaccct gtgcaacgcg gcggagatcc tgatacggaa gggcgcgacg
    14701 gaggtctatg cggcggggac gcacgctata ttctccggcc cggcataccg caggatcgaa
    14761 gactcctcca taaaggaggt cgtcgttacg gacacgttgc ccttgaagga gggcgagccg
    14821 cggtctaaga tacgcacgct tactatagcc ccgatcctcg ctagcaccat caagaacgtc
    14881 ttcctggacg actcggtgag cgaggtcttt atgggcgaga accagctttt ctaggatcca
    14941 gcagagtttt aggagattgc agtggcgaat aacgtggact tgcaggcgaa gaagcgcgag
    15001 ggacggggga agaacgactc ccggcgcctg aggcgcgagg ggatggctcc ggcggtcctc
    15061 tacggcgacg aggggagcac ggccatcgtg gtcccggcca ggacggttga ctacaccctc
    15121 cagcacgtcg gggacaacgc tctctacgac atagacctcg gagatggcgc cgcgacggcc
    15181 cgcgtcgtgg acgtgcagcg caacccggtg agcggcatcc tcacgcacgt ggacttcgcg
    15241 ccggtgaaca tgcgcgagcg tatcgaggtc acggtcccct tgcacatcgt cggtgaggcg
    15301 ccgggtgtcg aggagggcgg cgtgttgcag caggtcgcgt acgagatcca ggttgaatcc
    15361 ctccccggcg acatcccgca ggaggttacg ctcgacgtgt cgagcctcgg catgaacgag
    15421 aacctcacgc tcggggacct gacgctcccg gagggtgtgg atctcctctc cgagccggaa
    15481 gatgtcgcgg tgacgatcac gcagccgacc gagatctccg aggaggagat ggaggccgcc
    15541 ggcatcgtcg aggagccgac cgacgaggaa gaggccgccg ctgccgagga ggccgcagag
    15601 ggcgaggagg ctgccgaggg tgaagaggcg tccgccgacg agggtgagga acccgagggc
    15661 caggacgagc aggcttaacg agctgcgcca gtccttcctg gaccgcagga accggcaaga
    15721 agagagcggg ggaccgagaa ggtcccccgt cgtcgtgggg ctcgggaacc ccggtcgctc
    15781 ctacgagcgc acccgccaca acgccggcta cctggtcgcc gacgaactgg cgaagcgcca
    15841 caacggctcc tggcgtaaaa agaagaagtc cgaagccgct ccgatcagcc tcggcctcaa
    15901 gaacgtaacg ctcctcaagc ccactaccta catgaacaac tccggctcgg cgctctcgga
    15961 ctcccgcgtc gaagacctta tcgtggtcca tgacgacctg gacctcgaag aggggaccgt
    16021 gcgtgtgaag gttggcggcg gcgccggtgg ccacaacggc ctgcgctcca tcatccaaaa
    16081 caagggcaac gacttcacgc gggtccgcat cggcatcggc cgtcccccga taggcgttac
    16141 cgtaactgac tacgtgctgg gcaggatgga ctccgccgtg aaggacgcca tcccgaccgc
    16201 tgccgatgtc gtggagttcc tcgtcgagaa cggcgcggag gctgcgatga accgcttcaa
    16261 cgttcgttcc tgaagacgca tcggcaagac acgcgcaaaa agatgtcaca accgggcgtg
    16321 gcgtagatcg ggaggcaggt ctctcggcca catagctacg aactcctgag tagacaattt
    16381 ttgtcacaga attagcaaat ttgggtcata cgttgctccg gtcaggatat gtaggatgcg
    16441 ctactaacaa gccggacatg gaggaggaga gcgctccgct cctctggtaa gcgcaaggag
    16501 gcgagaagtg gcccgggtta tccaagaacg ctcaacagaa gaagcaggca ccacgaacca
    16561 atcgggaaag gacgccatga aggcgatcgt acgatacaag tatggctcac cggacgttct
    16621 ggagctccaa gatatcgaca agccccagat ctccgacgac gaggtgctgg ttcacgtgca
    16681 tgcggccggc gtggaccggg gggtgtggca cctcatgacg ggcctggcgt acccgatacg
    16741 cctcgcgggc tacgggttgc gggctccgaa gaaccccgtt ttgggcggag atctcgcggg
    16801 ggtagttgtg gcgatcggca agagcgtgac caggttccgg ccgggcgacg aggtgttcgg
    16861 catcggcgag ggctcgtacg ccgagtacgc acgcgcgctc gagaagaagc tcgcgcccaa
    16921 accggcgaac ctcactttcg agcaggcggc ggtggtcgcc atctcaggct tgactgccct
    16981 gcaagccgtg cgcgaccatg ggaaggcgcg cccggggcag aaggtgttga tcatcggtgc
    17041 ctcaggaggc gtggggacct acgccgtgca gatcgcccgg gtgttcggcg cgcgggtaac
    17101 cggcgtgtgc agcaccgcga aggtggagat ggttcgttcc atcggcgcgg atcacgtcat
    17161 cgactacacc aaggaggact tcgccgaagg agggcagcgc tacgacctga tcctggacat
    17221 cggcggaaac tcgccgctgg cacgccttag gcgcgccctc acccccaagg gaacgctcgt
    17281 catcgtcgga ggcgagagcg atgggcggtg gctcgggggc acagatcgcc agcttagggc
    17341 gatgatgctg tccccgttcg taagccagaa actactcgcg ttcatcaata aggagaacca
    17401 cgaggacatg ctcgtcctca aagagctaat cgaggccggg aaggtcacac cggtcctcga
    17461 ccggacttac ccgctcgccg aggccccgga tgccatccgg tatctgacag agggacacgt
    17521 cagaggaaaa gtcgtcatca ccatctgatg cgcgcaaagc agcgctcaaa ccacgggtct
    17581 cgcgaccccc gaggagagga cagggcaatg gatgtccacc cgctcacgtc atcgaccccg
    17641 cgaacgacca ggaaagggca acagggaacg gcacacacca ccgctgatgt atcgctccgc
    17701 ggagcagcag cggttgcagg gctcgggctg ttggtcatgg ccgtcctcgc gttcgcaagc
    17761 ttcgcggtct ttgggaacct ggtcgccacg ggagatgcgg caaagacggc acggaacatc
    17821 gtggaccatg agctgctctt tcgggccacc atctgcggtt tcttgatcgt cgcggtcctc
    17881 gatgtggtgg tggcgtgggc cctctacgtg ctccttaggc cggcaggcag gagcatcgcg
    17941 ttgctcgcgg cgtggttcag ggtggtgtac gcggctctct tcgcggccgc tttgagcaac
    18001 ctcttcgtcg ctgcgcgcct tcccaccgac gccgactcct tggagtcatt cggagcagga
    18061 cggttgaatg ctcaggtgct gatgtacgtg aacgcgttcg acgatggcta ccacgcggcg
    18121 ctggcgatct tcggccttca tctgctcgtg ctcggttacc tggtgttcaa gtcgggctac
    18181 atacctagag ttctcggtat cctggtgatg atcgcttcgt ttggctatct gatagatggc
    18241 ttcggcgggt ctctctcggc aggctacaac gccaacctta tccagttcac cttcatcggg
    18301 gaggtgttgc tcatggtttg gctcttgtgg aaaggtgtga ggcttaagaa ccaccctcag
    18361 cgtgagatct agcctcgtcg gtcgtagccc tgcttgccgg aaccgccaga gtatgaacgt
    18421 ttcaggagtg accgtataac tacaggacgc taggagatga atatgaagaa ggtttgcatc
    18481 gtgggcgcct ccgagaagct cgggcaatac atggtgcagc acgcgctgga tcggggctat
    18541 gaggtggttg gcgtgtgccg ggtaggcctg atcctgagcg ttcccgtggt cgccgtgggc
    18601 attatcgaga ctttcaccgc acgcacgacc acactcacct tcacttacgt ggttctagcg
    18661 ctattgaccg atctatgggc catagcggta ggtatatgga tggcccggag agcatggaaa
    18721 aaggaaacag aagaggaggg acaaacacgg aaaccacaga gcttacgttc ttaaatgcag
    18781 gagagttggc tggagaatag aggccaaaat ggcggattcg agacgtttag ccgggttgat
    18841 cggtcccacg atgctcgtgc tcggtgtctc cgaagcggtg aactttcgta tctgggagac
    18901 taacgtcgct ccgctcatct acctcaacgg gctgttgttg ttcgttgccg gcctttcgat
    18961 cgtccgcgtt cacaaccttt ggatgcgtgg ctggcccgtg atggtgaccc tcgtgggctg
    19021 ggttgccgtt ctcggcggtc tgttccggat gttcgctccg gaagtccaac aggccggtca
    19081 gaatgctccc acgaccatca tcaccgcgag cctggtcggc gctatcgggc tgttcttgac
    19141 cttcaaagcc tatggaccgg tggcgcgctc gaagcccacc ggttccgggc aggatggtgg
    19201 gcgacagcta gcggatcgcg ggcaggcgtg acgcaccgtt gcgataaccc ggagctcgcg
    19261 cttgaccaga cgtggcggcg ccggcgaagg acatccttac cggggatctc gtactgcgtt
    19321 tctacgtggc tctcgtcgcc tcgccggtcc cggacggttt cgtgcgctat cggtgaaagg
    19381 agttcttgag atgactactc ggaccttctc ctggctgaaa gaccatcccc tcgtcgtgtt
    19441 cttgttgggc gttgctttgg cctacaacgt cctcatgaac aacacggcgt tccgtcgctg
    19501 gttgtaccgg ggacggcgac caaaccggat cgcgaaggtc atgaacagag cggcggctgc
    19561 catggcctcg tcgggtgtcg cctccggccc catggtgacg ttggaggtga gaggacggaa
    19621 atccgggcgg accatctcgc tgccgctggt aatggcctct gtcgatggac agcgctacct
    19681 ggtgtcgatg ctcggagacg acgtgcaatg ggtccgcaac gtgcgagcga ccggtggaag
    19741 agcggtcatc cgtaccaggg accgcgaggc ggtccgactc gaagaggttg ccgcagagca
    19801 gcgcgcgccc atcttgaagg cttacctgaa gagagcgcca ggcgccagat ggcactttcc
    19861 gatcgacaag gaagctccgc tctccgagtt cgagcgggtg gcgccccgat ttccggtatt
    19921 ccgggttgta ccagggaatg cggcttgaga gcgccggacg taacgttccg ctgcaaccac
    19981 ggcggcgggt ttcgtttgcg gacgcgacgg ttctcccctc gaccgcctcc gagaccttcc
    20041 ggcatttcgg gataccgccg actttcaccg tcgtgccaac ttgcggtcct tggctcattc
    20101 ctaatgaggg tttgttcgac cgcctacggc aaccgcctac ggagcggggg catcctcggc
    20161 cgtctggtac ggaacgcagg aactggacaa gattcgtcac acaaatagcc aatctgggtc
    20221 atgcgtcgct atgcccaaga gatgcaacat tctgataatc gaccgaacga cgctaggagg
    20281 ttgtgatgtc aacgatcaag gaggccgcgt ccgagttcct ggcccgcagg cgggtggccg
    20341 tgaccggagt ctcgcgagaa ccgcagagcc acgggagcaa cgtcgtctac cggcgcttcc
    20401 gggagcgggg atacgaggtg ttcgccgtca acccgaacgc ggacgaggtc gaaggcgacg
    20461 cctgctatca cgacctgcgc tcgatccctg gcggcgtcga ggcggtcgtg atcggaacgc
    20521 ggccggaggt cgccgaggag acgatgcgcg agtgcgccga gctagtcctc tgccacagtt
    20581 tggatgcacg gtttgaag
//
LOCUS       DASSMB010000140        16405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_792149, whole genome shotgun sequence.
ACCESSION   DASSMB010000140 DASSMB010000000
VERSION     DASSMB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16405
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792149"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VFJ72_08355"
     CDS             complement(<1..675)
                     /locus_tag="VFJ72_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7479508.1"
                     /translation="MIETPEWYNASVLVDRNLEAGRADKVAIYCGDEQVTYGELARRI
                     NRFGHALTGLGIDREDRVLLMLNDTPSFPTAFFAAMRIGAIPIPVNTLLKPADYRFFI
                     ENSRARAVVCDEMHHGTIQTALEDYDEEPVQVIVTNGRAEGAHVLEYLLESGEDELDP
                     VATHKDDPAFWLYSSGSTGKPKGAVHLHHDIIYTCETYARHVLEVSEDDMCFSASKLF
                     HAYGLGN"
     gene            complement(693..2132)
                     /locus_tag="VFJ72_08360"
     CDS             complement(693..2132)
                     /locus_tag="VFJ72_08360"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7479509.1"
                     /translation="MAEPFIDGGYAAFSRLGRMPVVDPATGEEVDTVPRSGAEEVDQA
                     VRSAYEAFPAWRDTPAAKRGEILGEAANAVLAAMKELAPMLTAEQGKPLREARLEIRR
                     FVHTLEHYVGLAKNIRGGYVPDLDVDTYGLIVRRPLGVIGAIVPWNFPTTLLGNKLGP
                     ALITGNVVVAKPADTTPLTTLRIAEIMHEAGLPAGVFNVVTGTGPEAGQALAEHPLVR
                     KIAFTGSTPTGKKLMTVAADGLKRTTLELGGSDPMIVCDDANLDKAASAASVGRFFNC
                     GQACLAVKRVFVFESVADEFIEKVAGKVKKLKVGPGTAEGVMLGPLHSPRQRDLLEEQ
                     VDDAVDSGAEVLHGAKRPEGDGFDKGNFYEPTLLVEPARDSRVAMEEVFGPALPVWRV
                     GDMEEALERANSSIYGLGSSVWTADLNRASDAAERLEAGYTWINSPQIVYDELPFGGW
                     KQSGYGKEHGIEALDYYTETKSVVVKRAT"
     gene            2414..2821
                     /locus_tag="VFJ72_08365"
     CDS             2414..2821
                     /locus_tag="VFJ72_08365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7479510.1"
                     /translation="MAEKDYRVAASEFLRVAGLEFEEIGGSRVRGSIELGEHLHTPWG
                     VVHGGVYTTAVESAASVGASAAVEERGQFAVGVNNSTDFLRPMKEGRVEVVAEPIMQG
                     KSQQLWQVIITRSEDGKEVARGQVRLQNVPLPS"
     gene            complement(2838..3335)
                     /locus_tag="VFJ72_08370"
     CDS             complement(2838..3335)
                     /locus_tag="VFJ72_08370"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET7479511.1"
                     /translation="MPDIPEREREVTMRFLAQPTDINFGGKVHGGAVMKWIDQAGYAC
                     AVGWSGQYCVTVYVGGIRFYRPIRIGNVVEVRARLAHTGRTSMHVVVDVGASDPRDRR
                     RTETTHCVIVFVAVDDEGNPVEVARWRPETEEDAALERYAIKLMELRRDMEGTVADLR
                     GMAPP"
     gene            complement(3354..4502)
                     /locus_tag="VFJ72_08375"
     CDS             complement(3354..4502)
                     /locus_tag="VFJ72_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET7479512.1"
                     /translation="MVDIAVVGAGTMGAGISESAALAGMPVLMMDIREDALDRGLKTI
                     ERDLERRVKKGRLSEDESREVLGRVATTTSVEDCSSAPLVIEAVVEDMDIKRKVFADL
                     EGVVGGEAVLATNTSSLSVAGIAAVAERPERVVGMHFFNPVPAMRLVEVVAGPSTGPS
                     AVARAEEVSERLGKTPVRVSDTPGFIVNRVARPFYLEALRLVEAGGDPRRIDAAIREA
                     GFRMGPLELADLIGHDINLAVSESLFERYYYQPRFRPSHLQRSMVEAGMLGRKSGRGF
                     YDYGSEEKPANEGGVTEPSEDVALRVISCIVNEAFLALSEGVARSEDIDRAMKLGANY
                     PKGPFEWAEEAGARTILQTLDSLREVYGDAYLAAPLLRRRGQDRPDFD"
     gene            4621..5430
                     /locus_tag="VFJ72_08380"
     CDS             4621..5430
                     /locus_tag="VFJ72_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7479513.1"
                     /translation="MKDRSFEQIEVGVEGGIATITLNRPSRYNALGSRVVEELGGALE
                     DVEGSGEVRVLILTGAGERAFCSGVDLKERAAMDADERWTHNRALNAFAERLARLQIP
                     TIAALNGLAFGGGLEITLACDFRIAVENATFALPEVGIGIVPGAGGTQRLPRLVGPTR
                     AKELILTGRRIVAGTALEMGLVSKVVPQGSLMEEARALAAEISANSPLAVAYAKAAVD
                     LASETSMEQGLRYETAAIRATLASQDYSIGLAAFAEKRKPEFPPLTARRVT"
     gene            complement(5551..7362)
                     /locus_tag="VFJ72_08385"
     CDS             complement(5551..7362)
                     /locus_tag="VFJ72_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479514.1"
                     /translation="MSKRIAELRAGLWRTRDTLSNFTPFLRPRFGGLLLAFFVLMLET
                     GTSLALPWPLALTLDHVLKENPDPLPVDFVGPAALLAGIAFLLIILTAASRGLASYRR
                     YLLQKLGQQTVFDMREALYSKVHDLGLDYHGSKRTGDTITRVTSDVKEVRTMVVDSIV
                     EVGSSFLILLGMLVVMIWLSPWLTMLALLTVPFLFLAVVRYRKALIERMRVVRTKEGA
                     IASVIQEAITGIRAVKIFGREEDELERFRRESADSLEASIDSSLIEAKFSIILGLVGG
                     LGTALVLYFGARQVLAGALSIGDLTVFVAYLALFLSPLWALSRQVNQIGKSLVSGERI
                     VELLQAEPSVKDLPGARPAPRLDGRVSFEDVHFSYEEEAGEVLRGVSFEVEAGSRVAL
                     VGVSGAGKTTVTSLIARLYDPQRGRVMIDGQDIRDFTLKSLRDSITFVPQEPLLFRAT
                     VAENIGYGKAGASRQEIERAAALAGADGFIREMPQGYDTMLSERGESLSGGQRQRISI
                     ARAMLRESPVLILDEPQSGLDAEAADAVETHWRDLTGGRTTFVIAHELRLVRSVDQIL
                     VLEEGRVAESGAHEELVSSGGLYARLYALQERDTVQP"
     gene            7437..7811
                     /locus_tag="VFJ72_08390"
     CDS             7437..7811
                     /locus_tag="VFJ72_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479515.1"
                     /translation="MTRRVIAAIEDLLFKSKVSETAAHLGIEAKFPRSPEKLLAAVRD
                     APPDLLVLDLNSARFEPLDLLRSVKSDVATRDVPTVGFLSHVQKDLAVAAREAGCDRV
                     MARSAFTKDLPEILGGEAGGRG"
     gene            7798..8367
                     /locus_tag="VFJ72_08395"
     CDS             7798..8367
                     /locus_tag="VFJ72_08395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7479516.1"
                     /translation="MAVGSGGLGAWERIGSEKLFENPYFTLRSDSYRLPDGAVKDPYY
                     VLERPDAAIIFPVTKEGEVVLVRQYRPPLERMELGLPAGLVERGEDPEAAARRELLEE
                     TGCAGGEWEALGAIASSPSLKDNWAYLFLARGVEEVAESDPDEHERLEVVRAPVGKMR
                     SLVRSGEIVSSSGVAAVMLALERIEAPEA"
     gene            8598..9878
                     /locus_tag="VFJ72_08400"
     CDS             8598..9878
                     /locus_tag="VFJ72_08400"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HET7479517.1"
                     /translation="MVEGQPEGHVVRSCYIGMTIGERIGLSEDLRSALFYALLLKDAG
                     CSSNSFRTAVLFDADDFAAKRSFKTVDWSRLPNAVLYAARNVSPEGSVWAKARRFFSV
                     GKVLPDVGRELIQIRCERGAEITRLMGFSEETAGAIRNLDEHWDGGGHPDGLKGDEIP
                     FLARICGLAQTAEVFYSAYGPERAERVVRARRKRWFDPDLVDVFLAEARVGNIWENLR
                     GDITGAISRMEPEDRSLLATPERLDLAAVAFAQIIDAKSPYTFQHSEGVADAAVAISA
                     HMGLGPEELRNQRRAGLLHDIGKLGISNSILDKGSSLTDEEFAKIKTHPRLSYEILSR
                     VPPFESIVDRAANHHEKLDGSGYPRGLTGEDLDLPSRILTVADIYDALAQDRPYRRAP
                     PKERVMEIFDDESGTKLCPESVTALKELVSKGVL"
     gene            complement(9943..10287)
                     /gene="erpA"
                     /locus_tag="VFJ72_08405"
     CDS             complement(9943..10287)
                     /gene="erpA"
                     /locus_tag="VFJ72_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET7479518.1"
                     /translation="MDQKQETIVSITDNAAEKVKALMAQEGEEDLALRIGVRPGGCSG
                     FQYSIYFDDEVADDDQVFEAKGVRVLIDAMSVPYIVGSEFDYVDGLMGAGFAVNNPNV
                     QGGCGCGSSFTC"
     gene            complement(10375..12621)
                     /locus_tag="VFJ72_08410"
     CDS             complement(10375..12621)
                     /locus_tag="VFJ72_08410"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HET7479519.1"
                     /translation="MRGARVWLPLCAAGLAAGGAFSILFTGDTYDGSTGGFTGSSLVA
                     LTGYGAFWTMLFAALCAVLVGSAGSYRFVLVLPTAALYTQLAVYGLPPFLSLTGWRRH
                     LLEVGGDVYRAADTMYSEPIPYDLAPGLLVVLIPVVIIVVAFATSATLYEESPIFSIV
                     VLGVTIGVLSTISFEAGAGPFFFVFLVSALALLLVTGGTGHMGRAAVAAGLAVAALVL
                     VLPKAPFADQTVSNGLIDWTRIGTGGTSQLDVQADVGDYLTYGRDTELFRVRSPEALY
                     WRAGTLDYFDGAQWRDTTDPGLSYGEEISPEIETQIVPQYFTVMNSEMRRIFGAYKIV
                     QPSLDPSDMQQNSDGSWSAERPLTEGSEYRVISEVPQPTEAQLRSAGTAYPREIRDKY
                     LQLPDNPPEAVAATADRIRRNYSTRTPYDTARAIEKYLVYDGNFTYNLNVNYRRADQA
                     IEEFLGDGKEGFCTQFATSMVLIAREMGMPSRVVYGATSGEEVAENEYAVTGANMHLW
                     VEIYFPNVGWYPFNPTPGFTTPSTMEANAPRPRVSGNPANLIPDNQAQRQNQQQEQRT
                     PQEKQKEAPAKKDQKSSGSGVTLPAWPLYALVPALLAAAVPLTKRALLARGRPEDLYR
                     DLTGRLRDVPSSGGAIADSPALTPNERLRLLAGAAGVEEGPFAEFGRAYSAHLYSAEG
                     RRSTGRAVSAAYRRAVRAYETIPLWRRALGAVNPASLGARAARSFAAASAGAAKRARA
                     RFQRLRRR"
     gene            complement(12618..13898)
                     /locus_tag="VFJ72_08415"
     CDS             complement(12618..13898)
                     /locus_tag="VFJ72_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7479520.1"
                     /translation="MRALLRAFRRRGVRLSVRPTPRGWQAIIIGALVLVVARVIGTTQ
                     FHQLGYALLLLPAASFVLGFLASRGIGFSRSLPPGERLTAGKTARINVLISNRSRFDT
                     SRLRVTDRLPDPREISAPPIRGGGGTAVEVPLSFARRGVYELGPAEVRTADPFGLLGF
                     ARRFVGKTEVVVYPEVHDLTGFPLGGGNVEAGSRGSRGQRGDEFAGLREYRRGDDRRH
                     IHWKSVARTGELFVKEFALEAPRRYTVALDLRRSGIRTAEAEVEDAVSAAASVLTHLQ
                     KEHLPSRLLCNDHDSAKTEFGTDEASYWRAMRILATARADGEALLGETVLEERASLGE
                     GILIVSRARDESLAGCARKLRGAGLSVVVVMIAAHAYRGHAAGHPGVREEERESEFLR
                     EVGRLEAAGAEVISVRRPDGVAGLSSERSRGAVS"
     gene            complement(13904..14866)
                     /locus_tag="VFJ72_08420"
     CDS             complement(13904..14866)
                     /locus_tag="VFJ72_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7479521.1"
                     /translation="MDYNDFSQKVNRIVSNVGRAVSGKSESVSLAVVAMLAGGHVLIE
                     DVPGVGKTLLAKSLARSIRAEFRRIQFTPDLLPADVTGLNVYNQGEGEFEFWAGPVFA
                     NVVLADEINRASPKTQSALLEAMEEGSVTVDGQTRMLPQPFGVVATQNPVEHEGTYPL
                     PHAELDRFMIKMDLGYPDDRAETEMLKLSRDPVADLSPVVSAEEFLQMRRLVEEVHAS
                     EAVLRYVVSVTSATREHRDVYLGASPRASRMLLAASRARAAASGRSYCTPDDVKALAP
                     AVLSHRIIPSAEARGSSNGFAGGPGVAAEVVREILRSVPVTEAV"
     gene            complement(14935..15486)
                     /locus_tag="VFJ72_08425"
     CDS             complement(14935..15486)
                     /locus_tag="VFJ72_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET7479522.1"
                     /translation="MARCLTLNASYEPVALVSVKRAIVLVVSEKAEIVEANLERRFRS
                     ERSEYPYPLVIRLIKFVEIPRRLRRHVTNTILFARDNYRCQYCGKHRDDLNRRDCLTR
                     DHVKPISRGGLNTWDNVVTACTKCNARKGNRLPMECGMYPKSTPKEPRYVVMVWYSRG
                     LNEVQRRYVEQFYGEASLGEPAV"
     gene            15728..16165
                     /locus_tag="VFJ72_08430"
     CDS             15728..16165
                     /locus_tag="VFJ72_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7479523.1"
                     /translation="MSAEENKAIVRRVIEECFNQGNLDVIDELIAPDYVDHDPAMPEE
                     VRGPEGFRQFVSMYRSAFPDIHIQIKDQVAEGDRVAMRWVATGTHDGELIGIAPTGNR
                     VTTPGMSIDRISGGKVAETRDNYDAMGMMTQLGVIPAPQEAQA"
BASE COUNT         2825 a         5556 c         5363 g         2661 t
ORIGIN      
        1 gttcccgagc ccgtaggcgt ggaagagctt cgaggccgag aagcacatgt cgtcctcgga
       61 gacctccagc acgtgccggg cgtaggtctc gcaggtgtag atgatgtcgt ggtgcaggtg
      121 gaccgcgcct ttcggcttgc cagtggagcc ggagctgtag agccagaacg ccgggtcgtc
      181 cttgtgcgtc gccaccggat ccagctcgtc ctcgccggac tccagcaggt actccaggac
      241 gtgcgctcct tcggcccggc cgttggtgac gatgacctgc accggctcct catcgtagtc
      301 ttcgagcgcc gtctggatcg tcccgtggtg catctcgtcg cagaccacgg cgcgggcgcg
      361 gctgttctcg atgaagaagc ggtagtccgc gggcttgaga agggtattca ccgggatcgg
      421 gatcgctcct atccgcatcg cggcgaagaa cgccgtcggg aaggacggcg tgtcgttgag
      481 catcagcagg acccggtcct cgcggtcaat gcccaacccc gtcagcgcgt gtccgaaacg
      541 gttgatgcgg cgggcgagtt ctccgtaggt aacctgctcg tcgccgcagt agatcgctac
      601 cttgtcagcg cgcccggcct ccaggttccg gtcgacgagg acgctcgcgt tgtaccactc
      661 tggtgtctcg atcatgaggg gttcacctct cctcaggtcg cgcgcttgac gactaccgac
      721 ttcgtctccg tgtagtagtc gagcgcctcg atgccgtgct ccttgccgta gccggactgc
      781 ttccagccgc cgaagggcag ctcgtcgtag acgatctggg gggagttgat ccaggtgtac
      841 cccgcctcca gcctctccgc cgcatccgaa gcccggttca agtccgccgt ccagaccgag
      901 gacccgaggc cgtagatgga gctgttcgcc cgctccagcg cctcctccat gtcccccacc
      961 cgccacacgg gcagcgccgg tccgaagacc tcctccatag ccacccgcga gtcgcgcgcg
     1021 ggctcgacca gcaacgtcgg ctcgtagaag ttccccttgt cgaacccgtc tccctcgggc
     1081 ctcttcgccc cgtgcagtac ttccgcgcct gagtcaacag cgtcgtcgac ctgctcctcc
     1141 aggagatccc gctgcctggg agagtgcagc ggcccgagca tcacgccctc cgcggtcccc
     1201 ggcccgacct tcagcttctt caccttgccc gcgaccttct cgatgaactc gtccgccacg
     1261 ctctcgaaga cgaagacgcg cttcacggcg aggcatgcct ggccgcagtt gaagaaccta
     1321 cccacgctcg ccgcgctcgc cgccttatcc aggttcgcgt cgtcgcagac gatcatgggg
     1381 tccgagccgc cgagctccag cgtggtccgc ttgagaccat ccgccgccac cgtcatcagt
     1441 ttcttgccgg tgggcgtgga gccggtgaag gcgatcttgc gcaccagcgg gtgctcagcg
     1501 agcgcctgcc ccgcctccgg tccggtcccc gtcacgacgt tgaagacgcc cgccggcaga
     1561 ccggcctcgt gcattatctc cgcgatcctg agcgtggtca gcggggtggt gtcagcgggc
     1621 ttcgccacga cgacgttccc ggtaatgagc gccggaccga gcttgttgcc gaggagcgtc
     1681 gtcgggaagt tccacggcac gatggcgcct atcacaccga gcggacggcg tacgatcagg
     1741 ccgtacgtat ccacatcaag gtcgggaacg taaccgcccc ggatgttctt ggcgagcccg
     1801 acgtaatgtt ccagggtgtg gacgaaacgc cggatctcca gccgcgcctc cctgagcggc
     1861 ttgccctgct cggcggtcag catcggagcc agttccttca tggcggccag cacagcgttg
     1921 gccgcctcgc cgaggatctc cccgcgcttc gccgccgggg tgtcgcgcca cgccgggaac
     1981 gcttcgtaag cgctgcgcac cgcctggtcc acctcctccg ccccgctccg gggtacggta
     2041 tccacctctt ccccggtagc cgggtccacg accggcatcc ggccaagccg ggaaaacgca
     2101 gcgtagccac cgtctataaa cggttccgcc atacgaacac tcctccttta aacccgtaac
     2161 ctgtgccagg tccttcagac ccggccagcc ctagtccatg atatacccgt cacaactccc
     2221 ggcaagagca ggacagacac tcatacggac cgcgactcgt ttcactgttt ttagtctaac
     2281 cgatcagtca gtaatctgtc aaccgtccgg tcagtcaaat taatgcgggg gtggcgtacg
     2341 tatccgcgat gatctcctgc tatcgttctg ctgactggtt tgatgagggc gctacgcgaa
     2401 gaggaggcgg ccgatggcgg agaaagacta tagagtggcg gcgagcgagt tcttgcgggt
     2461 cgccggtctg gagttcgagg agatcggggg tagcagggtg cggggttcta ttgaactcgg
     2521 agagcacctt catacgccgt ggggcgtggt ccacggcggc gtctacacca cggcggtcga
     2581 aagcgcggcc agcgtggggg cttcggcggc ggtggaggaa aggggacagt tcgccgtggg
     2641 agtcaacaac agcactgact tcctgcgtcc gatgaaagag ggacgggtcg aggtcgtggc
     2701 ggagccgatc atgcagggca agagccagca gctatggcag gtgatcatca cccgcagcga
     2761 ggacggcaag gaggtcgccc ggggccaggt gcggctccag aacgtaccgc taccctcctg
     2821 aaaatccagc gcgcggatca gggaggcgcc attccccgga ggtccgccac cgttccttcc
     2881 atgtccctcc gcagttccat gagctttatg gcgtagcgtt cgagggccgc gtcctcctcc
     2941 gtttccggcc gccagcgggc gacctcgacg gggttcccct cgtcgtccac cgcgacgaag
     3001 acgatcacgc agtgggtcgt ctcggtgcgg cgccggtctc tcgggtcgct cgctcccacg
     3061 tcaacgacga cgtgcatgct ggtcctgccg gtgtgcgcga gcctggcgcg cacctcgacc
     3121 acgttgccga tccggatcgg cctgtagaag cggatgccgc cgacgtagac cgtcacgcag
     3181 tactgcccgc tccacccgac cgcgcaggcg tacccggcct ggtcgatcca cttcatcacc
     3241 gcgccgccgt gcaccttacc gccgaagttg atatccgtcg gctgcgccag gaaccgcatg
     3301 gtcacttccc gctcccgttc cggtatgtca ggcatgtagg ctacctctcc acctcaatcg
     3361 aagtccggcc ggtcttgccc gcgccgcctc agtagcggcg cggcgaggta ggcatcgcca
     3421 tagacttccc tcagagagtc tagagtctgg agtatcgtgc gagccccggc ctcctccgcc
     3481 cactcaaacg gacccttcgg gtagttcgcc ccgagcttca tcgcccggtc tatgtcctcc
     3541 gatctcgcca cgccctcgga taacgccagg aacgcctcgt tcacgatgca ggagatgacg
     3601 cgcagggcca cgtcctcgga aggttccgtg actcccccct cgttcgccgg tttctcttcg
     3661 gaaccgtagt cgtagaagcc ccggccgctc ttcctgccga gcatcccggc ctctaccatc
     3721 gagcgctgga ggtgcgaggg acggaagcgc ggctggtagt agtaccgctc gaagagagac
     3781 tcggagacgg cgaggttgat gtcgtggccg atgaggtccg cgagctccag cggacccatg
     3841 cggaaaccgg cctcgcgtat cgcggcgtcg atccgacgag gatcgccacc ggcctccacg
     3901 agcctgagag cttccaggta gaagggccgc gccacgcggt tcacgatgaa ccccggcgtg
     3961 tcggagaccc gaaccggcgt cttgcccagg cgctcggaga cctcctccgc gcgagctaca
     4021 gccgaggggc ccgtggatgg cccggcgacg acctcgacca gacgcatcgc cggcacgggg
     4081 ttgaagaagt gcatccccac cacgcgctcc ggacgttccg cgaccgctgc aatgccggcg
     4141 acggagagcg aggaggtgtt ggtggcgagc accgcttcgc ctccgaccac gccttcaagg
     4201 tcggcgaaga ccttgcgctt gatgtccatg tcctcgacca cggcctctat gacgagcggc
     4261 gcgctggagc aatcttcgac ggaggtcgtc gtcgcgacgc ggccgagcac ctcgcggctc
     4321 tcgtcctcgg agagcctgcc cttctttacg cgccgttcga ggtcacgctc gatggtcttc
     4381 aggccacggt ccagggcgtc ctcgcggatg tccatcatca agaccggcat gcccgcgagg
     4441 gccgcgctct ccgagatacc ggcccccata gtgcccgcgc caaccaccgc gatgtcaacc
     4501 acatcaccct cctgacggcc tgtcccctac cctcgcatct atatagcaga ttgtacgcgc
     4561 ggtcaggatt gtagacaata aggtccagcc ggtcatcgaa gtcggagagg agcgtgcggc
     4621 atgaaagaca ggagcttcga gcagatcgag gtgggagtcg agggaggcat agcgaccatt
     4681 acgctcaacc gtcccagccg ctacaacgcc ctcggcagcc gcgtcgtcga ggagcttggc
     4741 ggggcgctcg aagacgtgga gggttccggg gaggtccggg tcctcatcct gaccggggcg
     4801 ggagagcggg cgttctgctc gggtgtggac ctcaaggagc gcgccgcgat ggacgccgac
     4861 gaacgatgga cccataacag ggcgctcaac gccttcgccg agcgccttgc ccggctccag
     4921 atccccacca tagccgccct caacggtctg gccttcggcg gggggctgga gatcaccctg
     4981 gcctgcgact tccgaattgc ggtcgagaac gcgacgttcg cgttgccgga ggtgggcatc
     5041 gggatagtgc cgggcgctgg cggcacgcag cggctgccgc ggctggtagg accgaccagg
     5101 gcgaaggagt tgatcctgac cggcaggagg atcgtcgcgg ggaccgcgct ggagatgggc
     5161 ctcgtgagca aagtagtgcc ccagggctct ctgatggagg aagcgcgggc gctggcggcg
     5221 gagatctccg ccaacagccc gctggcggtt gcatacgcta aggccgccgt ggacctcgcc
     5281 tcggagacat ccatggaaca ggggttgcgc tacgagacgg cagcgatccg cgcgacactc
     5341 gcctcccagg actacagtat cgggctggcg gccttcgctg agaagcggaa gccggagttc
     5401 ccgccgttga ccgcccgccg cgtcacctga gaatcaacgt ccggagtcag gaagcgaacg
     5461 agatatatat ggcggccacg acgatggtca tgaccacgca gaggattatc agctcgcgca
     5521 tcgcacctcc ctacggcttc cttccctcat ctacggctgg accgtgtcgc gctcttgcag
     5581 ggcgtagagc ctcgcgtaga ggccgccgga ggataccagc tcctcgtggg cgccggactc
     5641 ggcgacgcgg ccttcttcga ggaccaggat ctggtccacg ctccgcacga gacgtagctc
     5701 gtgggcgatg acaaaggtgg tgcgtccccc ggtcaggtcg cgccagtggg tctcgaccgc
     5761 gtccgccgcc tcggcgtcca ggccggactg gggctcgtcc aggatcagga ccggactctc
     5821 acgcagcatc gcgcgggcta tggagatgcg ctgccgctgt cccccactca ggctctctcc
     5881 ccgctcggag agcatggtgt cgtagccctg gggcatctcc cgtataaagc cgtccgcccc
     5941 cgcgagcgcg gcggcgcgtt cgatctcctg ccgactcgcc cccgccttac cgtagccgat
     6001 gttctcggcg accgtcgccc ggaacaacaa cggctcctgc ggcacgaagg ttatggagtc
     6061 ccgcagggac ttgagcgtga agtcccttat gtcctggccg tctatcatca cgcgcccccg
     6121 ctgcgggtcg tagaggcggg cgatgaggct cgtaacggtg gtctttccgg ccccgctcac
     6181 cccgacgagc gccacccggc tcccggcctc tacttcgaag ctcacgcccc gcagcacctc
     6241 tccggcctcc tcctcgtagg agaaatgaac gtcctcgaag ctcacgcggc cgtcgaggcg
     6301 cggcgcggga cgggcgcccg gcaggtcctt cacgctcggc tcggcctgga gtagctccac
     6361 gatccgctct cccgatacga gcgacttgcc gatctggttg acctgcctac tcaacgccca
     6421 gagcggagag aggaacaggg cgaggtatgc cacgaagacc gtcaggtcgc cgatggagag
     6481 cgcgcccgcg agcacctgcc gggccccgaa gtagaggacc agcgcggtcc cgagaccgcc
     6541 gacgaggccg aggatgatgc tgaacttcgc ctcgatcagg gaggaatcta tgctggcttc
     6601 gagggagtcc gcgctctccc tgcggaagcg ctccaactcg tcctcctcgc ggccgaagat
     6661 cttgaccgcc cggatgccgg tgatggcctc ctggatgacg ctcgctatcg cgccctcctt
     6721 cgtcctgacg acccgcatcc gctcgataag cgccttccgg tagcggacga cggccaggaa
     6781 gaggaacggc acggtgagta gtgcgagcat cgtgagccag ggcgagagcc agatcatcac
     6841 gacgagcatg ccgagcagga tcaggaacga cgaccccacc tcaactatgg agtcaacgac
     6901 catcgtgcgg acttctttta cgtcgctggt gacgcgggtt atggtgtcgc ccgtccgctt
     6961 gctgccgtgg tagtcgagcc cgaggtcgtg gaccttgctg tagagagcct cgcgcatgtc
     7021 gaagacggtc tgctggccga gcttctgcag caggtagcgc cggtaggacg cgagtccccg
     7081 gctggcagcc gtcaggatta ttagcaggaa cgctattccc gcgagcagcg ccgccggtcc
     7141 cacgaagtcc accggcagcg ggtcggggtt ctccttgagg acgtgatcca gcgtcagggc
     7201 gagcggccag ggcaacgcga gactcgtccc cgtctccagc atcagcacga agaatgcgag
     7261 caacagcccc ccgaagcgtg gcctcagaaa cggcgtgaag ttggagaggg tatccctggt
     7321 gcgccagagc ccggcgcgga gttcagcgat tctcttggac acccggatag gataccgcac
     7381 gcgcggcaag tagaatgtgt caggagtccc acgtcttccc tggaggtccg ccacccgtga
     7441 cacggagagt catagcagcc attgaggacc tgctcttcaa gagcaaggtc tcggagaccg
     7501 ccgcccatct cggcatcgag gcgaagttcc cccgctcccc ggagaagctc ctcgccgccg
     7561 tccgcgacgc cccgccggac ctgctcgtcc tcgacctcaa ctccgcccgc ttcgagcctc
     7621 tagatctcct gcggtccgtg aagtccgacg tggcgacgcg ggacgttccc accgtcggct
     7681 tcctctcgca cgtccagaag gacctcgccg tcgccgcccg cgaggccggg tgcgaccggg
     7741 tgatggcccg gagcgcgttc acgaaagacc tgccggagat actaggcggc gaggccggtg
     7801 gccgtgggta gcggcgggtt gggggcgtgg gagaggatcg ggagcgagaa gctcttcgag
     7861 aacccgtact tcacgctccg ctccgacagc tatcgcctcc ccgacggcgc cgtcaaggac
     7921 ccgtactacg tcctggagcg cccggacgcg gcgatcatct tcccggtaac gaaggaaggc
     7981 gaggtcgtcc tggtgcggca gtaccggccg ccgctggagc gcatggagct gggccttccg
     8041 gcgggactcg tggagcgggg cgaagacccc gaggccgcgg cccggcgcga gctgctggag
     8101 gagaccgggt gcgcgggcgg cgagtgggag gctctcgggg ccatcgcctc gtcgccgagc
     8161 ctcaaggaca actgggcgta cctcttcctc gcccgcggcg tggaggaggt ggcggagtcg
     8221 gaccccgacg agcacgagcg cctggaggta gtgcgcgctc ccgtcgggaa gatgcggtcc
     8281 ctggtgcgct ccggcgagat cgtcagctcc agcggcgtcg cggccgtgat gctcgctctt
     8341 gagcggatcg aggcgccgga agcgtgaacg cagcagttgg ccgacctcca aacgcaggaa
     8401 aacgcaggaa ggatccgcga tacatccacg gcggagttgc gatcgtaacc actagagccc
     8461 tgcttgcgcg ggacactgat aggccagccg caaggtacga gcccgagagg tagagtcgca
     8521 taacaacccg gacgacacgc gagaaagagg tcttcaagtt ctcggaggtc ctcgccgcgc
     8581 tgtcccacgc gctcgatatg gtggagggcc agccggaggg gcacgtcgtc cgctcgtgct
     8641 acatcggcat gaccatcggc gagcggatcg gcctctccga ggaccttcgc tcggcgcttt
     8701 tctacgcgct tttgctcaaa gacgctggct gctcctccaa ctctttcaga acggccgtcc
     8761 tcttcgacgc cgacgacttc gcagccaaga ggtccttcaa gaccgtggac tggagccgtc
     8821 tgccgaacgc tgtcctctac gccgcccgca acgtcagccc ggaggggagc gtctgggcga
     8881 aggcccggcg gttcttctcc gtcggcaagg tcttgccgga cgtggggcga gagcttatcc
     8941 agatccggtg cgaacgcggc gcggagataa cgcgcctgat gggcttctcg gaggagaccg
     9001 cgggggcgat acgcaacctc gacgaacact gggacggcgg cggacacccc gacggcctga
     9061 agggcgacga gataccgttc ctcgcccgca tctgcggcct ggcccagacg gccgaagtct
     9121 tctacagcgc ctacggcccc gagagggccg aaagggtggt cagggctcgc cgcaagcgat
     9181 ggttcgaccc ggacctcgtg gacgtgttcc tggccgaggc gcgcgtggga aacatctggg
     9241 agaacctgcg cggagacata accggcgcca tctcccggat ggagcccgag gaccgtagcc
     9301 tgctcgccac gccagaacgc ctcgatctcg cggcggtcgc cttcgcccag atcatcgacg
     9361 ccaagtcccc gtacaccttc caacactccg agggcgtcgc cgacgcggcc gtcgcgatca
     9421 gcgcgcacat gggtctcgga cccgaggaac tgagaaacca gcgccgggcc ggtctgctcc
     9481 acgatatcgg caagctcggc atctcgaaca gcatcctgga caagggctcc tcgctaaccg
     9541 acgaggagtt cgcgaagatc aagacacacc ccaggctcag ctacgagatc ctgagccgcg
     9601 tacccccgtt tgagagcata gtggaccggg ccgccaacca ccatgagaag ctcgacggct
     9661 caggctaccc gcgcggcctc acgggcgaag acctcgacct cccttcccgc atcctcaccg
     9721 tcgccgacat ctacgacgcc ctcgcccagg accgccccta ccggagagcc ccgcccaaag
     9781 aacgggtgat ggagatcttc gacgatgaga gcggcaccaa gctctgcccc gagagcgtca
     9841 cggccctcaa agagctggtc tcgaagggcg tcctctagac ccggcaaaga aaaaggcccg
     9901 gagccgtgaa gccccggacc ttctgtttcg gactgttctg ctctagcagg tgaaggagct
     9961 gccgcagccg cagccgccct gcacgttggg gttgttgacg gcgaagccgg cgcccatgag
    10021 tccgtccacg tagtcgaact cgctcccgac gatgtagggg acgctcatgg cgtcgatcaa
    10081 gacccgcaca ccctttgcct cgaagacctg gtcgtcatcg gcgacctcgt catcgaagta
    10141 gatggagtac tgaaatcccg agcagccgcc ggggcgcacg ccgatgcgga gcgccagatc
    10201 ctcttcaccc tcctgcgcca tcagcgcctt gaccttctcc gcggcgttgt cggtgataga
    10261 gacgatggtt tcctgcttct ggtccatcag cacctcctgg gaatgctgta gcgatcggct
    10321 tcgatagcat gattattaca tcgccgctcc cgttggtcaa cgggcttccc cgctctaccg
    10381 ccggcgcaac cgctggaacc gcgcccgcgc tcgcttggcc gctccggcac tcgccgccgc
    10441 gaagctccgc gccgcccgcg caccgagcga ggccgggttg acagcgccga gcgcgcgccg
    10501 ccagagaggt atggtctcgt atgcccgcac cgcccgccgg taggcggcgc tcacggcgcg
    10561 gcccgtactc cgccgtccct ccgccgagta gaggtgcgcg gagtacgctc gtccgaactc
    10621 cgcgaacggt ccctcctcca cgcccgcagc tccggcgagc aaccgcagcc gctcgttcgg
    10681 ggtgagagcc ggagagtcag cgatagctcc tccagacgag ggcacgtcgc gcaggcgtcc
    10741 ggtgaggtcc cggtagaggt cttctggacg tccgcgggcg agcagggccc gcttggtaag
    10801 cggcacggcg gcggcgagga gcgccggcac gagcgcgtag agcggccagg cggggagcgt
    10861 tacgccggag ccggaggact tttggtcctt tttggcgggc gcctctttct gcttctcctg
    10921 tggggtccgc tgctcctgtt gctggttctg gcgctgggcc tggttgtccg ggatgaggtt
    10981 ggcggggttg cctgagactc gcggacgggg ggcgttcgcc tccatcgtcg agggggtggt
    11041 gaagccgggc gtcgggttga acgggtacca gccgacgttt gggaagtaga tctccaccca
    11101 caggtgcatg ttggcgccgg tcacggcgta ctcgttctcc gccacctcct cgccggaggt
    11161 ggcgccatag acgaccctgg agggcatccc catctcccgc gcaatgagga ccatcgaggt
    11221 ggcgaactgg gtgcagaagc cctccttgcc gtccccgagg aactcctcta tggcctggtc
    11281 ggcgcgacgg tagttcacgt tcaggttgta ggtgaagttg ccgtcgtaga ccaggtactt
    11341 ctcgatggcc cgcgccgtgt cgtagggcgt gcgggtggag taattacgcc ggatcctgtc
    11401 ggcggtggcg gcgacggcct cgggagggtt atccgggagc tggaggtact tgtcccggat
    11461 ctcccgcgga tacgctgtgc ccgccgaacg gagctgcgct tccgtgggct gggggacctc
    11521 ggagatgacg cggtactcgc tcccctcggt cagcggcctc tcggccgacc aggagccgtc
    11581 cgagttctgc tgcatatcac tcgggtcgag ggagggctgc acgatcttgt acgctccgaa
    11641 gatgcgccgc atctccgagt tcatcaccgt gaagtactgc ggcacgatct gggtctctat
    11701 ctcaggtgag atctcctccc cgtagctcaa tccggggtcg gtcgtgtccc tccactgggc
    11761 gccgtcgaag tagtccagcg taccggcgcg ccagtagaga gcctccgggc tgcggacccg
    11821 gaacaactcc gtatcgcggc cgtaggtcag gtagtctccg acatcggcct gcacgtcgag
    11881 ctgggaggtc ccgcccgtcc cgatgcgggt ccaatctatg agcccgttgc tcacggtctg
    11941 gtcggcgaag ggcgccttcg gcagcacgag cacgagcgcc gccacggcga gacccgcggc
    12001 aaccgccgct cgtcccatgt gccccgtgcc gccggtgacg aggagcagag ccagcgcgga
    12061 gaccaggaac acgaagaaga acgggcccgc cccggcctcg aagctgatgg tcgagaggac
    12121 tcctatggtg acgccgagca cgacgatgga gaagataggg ctctcctcgt agagcgtcgc
    12181 cgaggtcgca aacgccacca cgatgatgac caccgggatc aggacgacca gcaaccccgg
    12241 cgcgaggtcg tagggtatcg gctcggagta catggtgtcg gccgcccggt acacgtcccc
    12301 accgacctcc agcaagtgcc ggcgccaccc cgtcaaagac aggaacggcg gcagcccata
    12361 gacggcgagc tgcgtgtaga gagcggcggt cggcagaacc agcacgaaac ggtagctccc
    12421 cgccgaccca accagcaccg cgcagagcgc cgcgaagagc atcgtccaga aggctccgta
    12481 gccggtcagc gccacgagcg acgagcccgt gaagccgccc gtggaaccgt cgtaggtgtc
    12541 gccagtgaac aggatgctga aggctccgcc cgccgcaagc cccgccgcgc acagcggcag
    12601 ccagaccctc gcgcccctca cgatacggct ccccgcgatc gttcgctgga gagtcccgcg
    12661 acaccgtccg gacgccgcac ggagatcacc tccgcgccgg cggcctccag ccggccgacc
    12721 tcccgcagga actccgactc gcgctcttcc tcccgaactc cggggtgacc ggcggcatgt
    12781 cccctgtaag cgtgggcggc gatcatcacg acgacgaccg acaacccggc cccgcgcagc
    12841 ttccgcgcgc aaccggccag gctctcatcc cgcgcgcggg agactatcaa gatcccctct
    12901 ccgagggacg cgcgctcctc caggaccgtc tctccgagga gggcctcccc atcagcccgc
    12961 gcggtagcca gtatccgcat cgcgcgccag taggacgcct cgtcggtccc gaactccgtc
    13021 ttcgcagaat cgtggtcgtt gcacagcagg cgcgacggca agtgttcttt ttggagatgc
    13081 gtcaggaccg aggcggcggc ggatacggcg tcctcgacct cggcctccgc ggtccggatg
    13141 ccgctccggc gcagatcgag cgccacggtg tagcggcgcg gagcctccag ggcgaactcc
    13201 ttgacgaaca gctcgccggt ccgggcgacg ctcttccagt ggatgtgccg ccggtcgtcg
    13261 ccgcgccggt actcgcgcag gcccgcgaac tcgtcacccc gctgcccgcg cgagccgcgc
    13321 gagccggcct ccacgttacc gccgcctaaa gggaagcccg tcaggtcgtg gacctccggg
    13381 tagacgacga cctccgtctt gccgacgaac ctgcgggcga agccgaggag cccgaacggg
    13441 tccgccgtcc gcacctccgc tgggccaagc tcatagacgc cacgccgggc gaaggagagt
    13501 gggacttcga ccgccgtccc tccgccgccg cggataggtg gagccgagat ctcgcgcgga
    13561 tccgggagcc ggtccgttac ccgaaggcgc gaggtatcga agcgcgagcg gttggagatc
    13621 aggacgttta tcctcgcggt ttttccggcg gtcaggcgct cgcccggcgg caggctgcgc
    13681 gagaaaccta tgccccgcga cgccaggaac ccgaggacga acgaggcggc gggcaggagg
    13741 agcagggcgt acccgagctg gtggaactgg gtcgtcccga tcacgcgcgc caccaccagc
    13801 accagcgccc cgatgatgat cgcctgccac ccccgcggcg tgggccgcac ggagagccgg
    13861 acgccgcgcc gtcgaaacgc gcgcagcaag gcccgcacgg cggctagacc gcctcggtga
    13921 cggggacgga gcgcagtatc tcgcgcacca cctccgccgc gacgcccggg ccgccggcga
    13981 aaccgttgga cgacccgcgg gcctcggcgg acgggatgat cctgtgcgag agcaccgccg
    14041 gagcgagcgc cttcacgtcg tccggggtgc agtaggagcg cccgctcgcg gcggcccgcg
    14101 ctctggaggc cgcgagcaac atccggctcg cccgcggcga ggcgcccagg tagacgtccc
    14161 ggtgctcgcg cgtcgccgag gtaacggaga cgacgtagcg caacaccgcc tcgctcgcgt
    14221 ggacctcctc taccagacgg cgcatctgga ggaactcctc cgcgctcact accggggaga
    14281 ggtcggccac cgggtcgcgc gagagcttga gcatctcggt ctcggcccgg tcgtccgggt
    14341 atccgaggtc catcttgatc atgaaccggt ccagctccgc gtgcggcagc gggtaggtcc
    14401 cctcgtgctc gaccgggttc tgggtcgcca ccacgccgaa gggctgcggc agcatccggg
    14461 tctggccgtc caccgtcacg gagccctcct ccatcgcttc gaggagcgcg ctctgcgtct
    14521 tggggctggc gcggttgatc tcatcggcca gcaccacgtt ggcgaagacc ggccccgccc
    14581 agaactcgaa ctccccctcg ccctggttgt agacgttcag gcccgtaacg tcggcgggga
    14641 gcaagtccgg tgtgaactgt atccgccgga actccgcccg gatagaacga gcgagagact
    14701 tcgcaaggag cgtcttcccc acgccgggca cgtcttctat caggacgtgc ccacccgcga
    14761 gcatcgccac cacggccaga gagacgctct cgctcttgcc cgacaccgct cgcccaacgt
    14821 tggagacgat ccggttcact ttctgagaga aatcgttgta atccaaagaa gccccgccag
    14881 ttgttcggac gacgaaggga attatatcaa gggttcaaca gagcccggcc ggaactatac
    14941 ggccggctct ccgagcgacg cctcgccgta gaactgctcg acgtagcggc gctgcacctc
    15001 gttgaggccc cgcgagtacc agaccatgac cacgtagcgc ggctccttcg gcgtgctctt
    15061 ggggtacatg ccgcactcca tcggcagacg gttgcccttc cgggcgttgc acttggtgca
    15121 ggccgtcacg acgttgtccc aggtgttcag gccgccgcgc gagatcggct tcacgtggtc
    15181 gcgggtcagg caatcgcggc ggttcaggtc gtcgcggtgc ttgccgcagt actggcagcg
    15241 gtagttgtcg cgggcgaaga ggatggtgtt ggtgacgtgg cggcgcaggc ggcgtgggat
    15301 ctcgacgaac ttgatcaggc gtatcaccag cggataagga tactcggacc gctccgaacg
    15361 gaagcgccgc tccaggttgg cctccacgat ctccgccttc tccgagacga cgaggacgat
    15421 agccctcttg accgaaacca gggccacggg ctcgtagctg gcgttaagcg tcaggcatcg
    15481 tgccacgcga gcattctaac atagcaagat gcttggccgc gtaaactttc cgttacataa
    15541 ggcggatccg ccccggcgcc accggcatgc ttcgtatagt cccggaagag tctatccccg
    15601 gaagcccggt agagaccctg aaattagccc atattgagga tgtacggcgg tgctttgtcc
    15661 cgcctacaat agtactgggg taagcgaatg acccgcccgg gtagcgggtc gggaaggaaa
    15721 taaacatatg tcggcggaag agaacaaggc catcgtccgg cgcgtcatcg aggagtgctt
    15781 caaccagggc aacctggatg tcatagacga attgatcgcc cctgattacg tggaccatga
    15841 tccggccatg cccgaggaag tacgcggacc cgaagggttc aggcagttcg tcagcatgta
    15901 ccgctcggct ttcccggaca tccacatcca gatcaaagat caggtagccg agggggaccg
    15961 ggtagcgatg cgctgggtag cgaccggcac ccacgatggc gagctcatag gcatcgcccc
    16021 caccggcaac cgggtaacga cgccgggcat gagcatagat cgcatctcgg gcggcaaggt
    16081 cgcggagacc cgggacaact acgacgcgat gggcatgatg acccaactcg gcgtcatccc
    16141 cgctccgcaa gaggctcagg cgtaagaacc ccaggagccg gagggccggg gcttctatcc
    16201 gctccggccc ttgcgcggta catcggaccg caagacggag aacggaggtt acagacgagc
    16261 aactcggcag aaaagcagtg aaattcactc atttgagtga ttcgtctccc ggactttcgg
    16321 gcgatgatac gggagtagct tgcaggcgcg ggacgcgaga tgcccgccca ggggaaggag
    16381 gcttcgatga ctcgaagcgt agaga
//
LOCUS       DASSMB010000141        15466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_286235, whole genome shotgun sequence.
ACCESSION   DASSMB010000141 DASSMB010000000
VERSION     DASSMB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15466
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286235"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..486
                     /locus_tag="VFJ72_08435"
     CDS             55..486
                     /locus_tag="VFJ72_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET7479524.1"
                     /translation="MDRKSRIELLVDHVRDPRHQGVLDDADVIMPGGSPECGGSVVVY
                     LKGDGNGGIEALSWTGQGDTISMGASSIIMERVLAEEMDLDQVLALDYDELIEGLGRD
                     VIGSRTRNATLGLSTLKNAIHKYRRDRRSANLATEPVRSGG"
     gene            complement(524..943)
                     /locus_tag="VFJ72_08440"
     CDS             complement(524..943)
                     /locus_tag="VFJ72_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479525.1"
                     /translation="MRSWTSALYWVVAGAVLGLGLIGLMTIGLPFLVIGLAMALVGVW
                     KPGISGVWAVLIGFGGLPALFFLSHMVDTARSAANPYCNNVGPNAGEITIPPGVRMVE
                     CSSIPASYYVMFAVFAGIMLLGVFLGFLRRARSHSTA"
     gene            1035..2444
                     /gene="argH"
                     /locus_tag="VFJ72_08445"
     CDS             1035..2444
                     /gene="argH"
                     /locus_tag="VFJ72_08445"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET7479526.1"
                     /translation="MSERDRSPLWGGRFGSSPAEAFERLNASIPFDGRLAPYDIQGSI
                     AHAKMLGERGIVSSDEAAELVRGLKTVLREVETGEFSWTLADEDVHTAVERRLREVAG
                     DVALKLHTGRSRNDQVALDLHLFVREAAEDIRTGLLAVMRALVEVAEAHRDLILPGYT
                     HLQRAQPILLSHHLLAHFEALRRDLVRFEAARESANVSPLGAAALGGTPHPVGPALTA
                     AELGMRPLTNSLDAVSDRDFALDLLYACAVLGVHLSRLGEEWVLWTSQEFAFATLDDA
                     YSSGSSIMPQKKNPDAYELMRGKAGRLIGSLNAMLITLKGLPLGYSKDLQEDKEPILD
                     AVDTVLSTLAVLPEMLRTARFDGERMLAAAGGFALATELADFLAARGVAFREAHRAVG
                     RLVRRCEELGVSLEDVPPEELEAAHPALADLPRDLLTPRGSVANKRSAGSTSPGSVEN
                     QLERARGFLEMGGQAGRES"
     gene            2489..3313
                     /locus_tag="VFJ72_08450"
     CDS             2489..3313
                     /locus_tag="VFJ72_08450"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7479527.1"
                     /translation="MKIVQMSDVHVGSGLFRPDLLEATIEETNALEPDLVAVVGDLTM
                     EGYRWEFEGARAYFDRLECPNVVYGMGNHDARNVGYRHFEEFFGIRERSVEISVPEGT
                     AKVVSLDSTKPDLDEGEVGREHYNWLDSEFRGWDRGPRILMIHHHILAVPGTGRDVNN
                     LRDAGDVMAILRELKIDLVLSGHRHVPYVWSISGVRIVHSGTVSTMRVRGVMPPSYNV
                     IEFDEESVSIRLQEPGKGESGEQPLASFSRQPVSTSRFYPDFDNFVVFDRLPFLKD"
     gene            complement(3332..3688)
                     /locus_tag="VFJ72_08455"
     CDS             complement(3332..3688)
                     /locus_tag="VFJ72_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479528.1"
                     /translation="MPATVRIRPELITEHRLRIEMYGLEDEDIENTIRMKGWAWVLAR
                     RGWAYAGEPDFIYRQIREVVIALPDIVFEPDAIEESVKTVLEKARSDEEREEGRDLLR
                     RAFEKTGQLEEGGKYL"
     gene            complement(3775..4722)
                     /locus_tag="VFJ72_08460"
     CDS             complement(3775..4722)
                     /locus_tag="VFJ72_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7479529.1"
                     /translation="MKNKTMLIRAVTVLLVAALVSVVAACGGGSSGSSDSGSGKSVTV
                     SSKKFTEQILLGEMYAQAFENAGYDVKRKLNLGSEQVMDKSLKDGTIDVYPEYTGTAF
                     VAILKKDPKTAPDTEKETYDIVSKFYENRKDTPMKMLTPAPFDDNYGIVVRTSLAKKL
                     GLKTLDDLADKSGQLVFSSYSEFQNRSDGYPNMQKNYPKLDFKEIKVVNDLGIRYKAL
                     AENKADVAIGFTTDGQLASPKLTVLKDTKAIWPFYYPAPVTTQKFLKKNPDAEKILNE
                     VSASLDAKEMRKLNGEVDLEQKDPEDVAKQHLEDAGILD"
     gene            complement(4741..5394)
                     /locus_tag="VFJ72_08465"
     CDS             complement(4741..5394)
                     /locus_tag="VFJ72_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479530.1"
                     /translation="MDAFMEVLQRPDFTKNFFVHIELSLAAVLLAILLSLPLAIAVNR
                     SDLGSTVAINTGNIGRAVPSLAILVLMLPIFGLSFTTALVALTLLAIPPILINAVVGL
                     RQVSRQVLDAAKGMGLSGRQILTGIQIPIAAPIIFAGIRTSAVQVVASATLATFIGAG
                     GLGDYIVEGYQRNSTPIALAGALSVALLAVVTELVFGRLESGFTPKGLKIAQQRRGK"
     gene            complement(5395..6135)
                     /locus_tag="VFJ72_08470"
     CDS             complement(5395..6135)
                     /locus_tag="VFJ72_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479531.1"
                     /translation="MDSADDPPTAILAFLQSEFAPKSVQQKGFINWEWLADHLTETVL
                     PAFLQHVFLSFISVAIALVIALPLGVLASRRRNLYAPLTAIAGFLYTIPSFSFFTILI
                     FIVGLSVGRTPVIIALVAYSLLILIRNVVTGLDSVPAETIDAARGMGLTNRQILFGVE
                     LPLALPVIVAGIRIATVTVIGIAVIGSYIGGGGLGNLVFDGITRDFPTMYVTGAVLST
                     ALAILADLVLVRTERVFSPWSRRVRRAG"
     gene            complement(6128..7108)
                     /locus_tag="VFJ72_08475"
     CDS             complement(6128..7108)
                     /locus_tag="VFJ72_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479532.1"
                     /translation="MIEFNDVSKTYAGSQRPVVQDLSFTIPDGEICVLVGPSGCGKTT
                     SMRMVNRLIEPSSGEILIDGQKNTAMSGTQLRRKIGYAIQQIGLFPHRTIADNIATVP
                     NLLGWEKSRTTSRVDTLLDLVGLAPDQYRDRYPAELSGGQQQRVGVARALAADPPIML
                     MDEPFGAVDPITRDRLQNEFLNIQKDIKKTIVFVTHDIDEAIKMGDKIAILKEGGILA
                     QYDTPENILVNPASDFVQSFVGGDRVLKRLSLTRVGDIELEPANGNGHDLPRINAGWT
                     LRDALSEIIGAGADRGVVVQDGDENGGSSRGTITLDSIRKISHGAEASRG"
     gene            complement(7380..7658)
                     /locus_tag="VFJ72_08480"
     CDS             complement(7380..7658)
                     /locus_tag="VFJ72_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479533.1"
                     /translation="MGLIRTILALVILVILAHVGMVYAGVERATNGLTDALYSLGELL
                     ESPATIILNAISSSGQLSVDTGNFYVVALTAAAAYFVLYLLLGIGRRG"
     gene            complement(7933..8301)
                     /locus_tag="VFJ72_08485"
     CDS             complement(7933..8301)
                     /locus_tag="VFJ72_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479534.1"
                     /translation="MSLIIIFIGALILVISLMGVAFGIYMSTHPKTREPGKLFAIWWV
                     PGVAAAFGVFMRDSVTFAIGMICFLIAGAVLLLKSGTSGPSVKRKRGSTGRSGAKRTS
                     STRTTRENRTRGKGYRREAS"
     gene            complement(8357..8737)
                     /locus_tag="VFJ72_08490"
     CDS             complement(8357..8737)
                     /locus_tag="VFJ72_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7479535.1"
                     /translation="MTRAAAYIRTDPEGGLPAQEEQRSAIEDYAAANRYVLVEIYSDS
                     QAPGKLLYHKPGLKAAIDNIKEQEDWEILIVADPRCVSDDASALHELVHKFSLYNNRL
                     ESPLRSWDEFLSAMKSYRRALSRR"
     gene            8793..8866
                     /locus_tag="VFJ72_08495"
     tRNA            8793..8866
                     /locus_tag="VFJ72_08495"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8827..8829,aa:Pro,seq:ggg)
     gene            9074..9838
                     /locus_tag="VFJ72_08500"
     CDS             9074..9838
                     /locus_tag="VFJ72_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HET7479536.1"
                     /translation="MISSLEDLRVVPVDRLILHEAHDERRLASLRERITSEGVQRNPV
                     IVAPHESDYLVLDGAHRVHTMREIGCRLILVQIVEPPSAAESWGHLLTGMDIPELRSV
                     SEVAVREEPGGPSLATVEMAGGEPVYIEARGGDGLREDIRALWAIHALYPDGDVVRRV
                     DPDGPVEIGAGEALVRYRTFTVEELVEVVHCGTVLPAGITRFRFRERILGVRFPLEHM
                     RDGDVEAGNSELRRFVNEHWQQNRIRYYDEPVVLFE"
     gene            9936..11273
                     /locus_tag="VFJ72_08505"
     CDS             9936..11273
                     /locus_tag="VFJ72_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7479537.1"
                     /translation="MSGSDVTKAVMRGEISRRRFLKLGGAGLAGVAMLGAAGCGGDSE
                     QGSGGTTRLTFTFGPDASGGLQTLIDRFNKQNKGEIEVNWRETPAASDEYFEQVQSEL
                     QSGQSKIDVVGGDVVWPAQFAANGYLLDLSDRFTSEMQAKYLSGPLEPIKYEGKTYAV
                     PWFTDAGMFYYRKDLLEKSGFSEPPVTWDEMKDMIEKIRSDSGTKYGYVFQGAQDEGG
                     VVDALEHIWNAGGSVLEGNKVVVNSPEARKGLEVRRSMITGGFSPRASGDYSTQESQA
                     VFTDGNVIFMRNWPFVYGLLSNPDDSKVRPEQVDIAPIPVSEKGARSFSGLGGWNFMV
                     NANSEDKLDEIWKFIQFMTSPESQKTLALQSTRLPTLKSLYEDQELLDKVPVAARGKE
                     ALQSTRPRPISPYYSDMSLKMADSFNAALKGEVPVASALAKLQRDLQNIVDQG"
     gene            11487..12773
                     /locus_tag="VFJ72_08510"
     CDS             11487..12773
                     /locus_tag="VFJ72_08510"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET7479538.1"
                     /translation="MTATVVLGLAWGDEGKGRVCDALATDADYVSRYSGGNNAGHTIR
                     VGKEEFVVHLIPSGIVREGVICTVGNGVVVNPDVLAEEVTALEGRGISVRDRLKIDGR
                     AHLIMPYHIRLDSHREKALGKAKIGTTNRGIGPTYEDKVARAGIRVQDIFDEGILRTK
                     LKAALREKNAIFTSIYDEEPFDPDELADGLLRHRGLLEPMVADTGTMLRDALDRGEKV
                     LLEGAQATLLDNDFGTYPFVTSSNPSVGGVCVGAGIPPRELEHVIGVTKAYTTRVGDG
                     PFPTELRDETGDIIREAGHEYGSTTGRPRRCGWLDLVAVKWTARLNGITHLAVTLLDV
                     LSAVEEINICVGYEIDGERVEGFPMSQTDLHHAKPVYKTLPGWGVDITGCRMRGDLPE
                     AARDFVEFVEAEIGAPLCMISVGPERDQAIIERIGA"
     gene            12778..14052
                     /gene="purD"
                     /locus_tag="VFJ72_08515"
     CDS             12778..14052
                     /gene="purD"
                     /locus_tag="VFJ72_08515"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET7479539.1"
                     /translation="MRVMVVGSGGREHALVETLAASHIEPEMFAAPGNPGIARIAEVV
                     DIPADDLIGLRDFASENRIDLTVVGPEVPLIGGIAECFWEEDLRIFGPSRAAARIEGS
                     KVFAKELMKHAGVPTADFEAFDREGSALAYLRAHSDYPVVVKADGIAAGKGVMIARSR
                     EEAEEAVREVFSGKFGAAGERVLIEECLEGREASVFVMTDGVNILPFLPAQDYKRIGD
                     GDEGPNTGGMGAYSPLMWMEPATYAAILEEIIRPTLHQLSLIGAPYSGLLYAGVMVTE
                     TGPKALEFNCRFGDPETQVILPRLETDLLELMLAVNDETLAGREVEWLAEKAVCVVLA
                     SEGYPETSHSGDEIGGLENIPAGVYIYHAATEERDGKFYTAGGRVLNVVGTGPTVIEA
                     RARAYAAVEQIHFAGMQYRTDIALEAIELEDI"
     gene            14105..15418
                     /gene="purB"
                     /locus_tag="VFJ72_08520"
     CDS             14105..15418
                     /gene="purB"
                     /locus_tag="VFJ72_08520"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET7479540.1"
                     /translation="MIERYTLPEIGAVWTEEAKYRAWLKVEIAVCRARAQLGEIPSDE
                     VEELAEKADFTVERIHEIEAETNHDVIAFVSTVAEYAGDVSRHFHFGLTSSDVLDTAG
                     ALQLRDALDLILTETRGLTRLLAGMSLEHRDTVMVGRTHGVHAEPTTLGHKVAVWAFE
                     MERNLDRLSHAREVAAVGKISGAVGTYANVDPKVEALTCEELGLSSSPASTQIVQRDR
                     HAEVLASLAILASTLEKIALEIRGAQRTEIRELAEPFGRGQKGSSAMPHKKNPILTER
                     LCGMARLMRGYAAVGFENNALWQERDISHSSAERVVLPDATILALYMLRTTGRVLRGL
                     QVHEDRMLQNMNLGGGIVFSGRVLLALVEAGMSRDDAYKVVQGAAMRAWEGEGGFREL
                     LEESDEVRERLGEDLDGLFDPSYALRNLGVVFERVEELKERLGDG"
BASE COUNT         2795 a         4704 c         5241 g         2726 t
ORIGIN      
        1 ctggaccgag tggggcaact ccgtgcgcgc ccccatcgag aggtaaagag ccctttggac
       61 cgcaagagcc gtatagagct gctcgtggac cacgtcaggg acccccgcca ccagggggtc
      121 cttgacgacg ccgacgtgat catgcccggc ggtagtcctg agtgcggggg atcggtggtc
      181 gtctatctca agggcgacgg caacggcggg atagaagccc tctcgtggac cggacaggga
      241 gatacgatca gcatgggcgc ctcgtcaatc ataatggagc gtgtgctggc cgaagaaatg
      301 gacctggatc aggtcttggc gctcgactac gacgagctaa tagagggcct gggccgcgac
      361 gtcatcggca gccgcacccg caacgccacc ctcgggcttt ccacgctaaa gaacgccatc
      421 cacaagtacc gccgggaccg gcgctccgcc aacctggcaa cagagcctgt ccgcagcggc
      481 ggatagcttg cggcacggat ctctcacata acgcgccctg atatcaggcg gtcgagtgag
      541 agcgggcgcg tcgcaagaat cccagaaaga cgccgaggag catgatcccg gcaaagaccg
      601 cgaacatgac gtagtagctg gcgggaatgg atgagcactc gaccatgcgg acgccgggtg
      661 ggatagttat ttctccagcg ttgggaccca cgttgttaca gtaagggttt gctgcgcttc
      721 tggccgtatc gaccatgtgc gacaggaaga acagcgctgg aagtcctcca aaaccgatca
      781 ggacggccca cactccgcta attcccggct tccagacccc gacaagcgcc atagccagcc
      841 cgatgactag aaaaggaagt ccgatagtca tgaggccgat caacccgagg cccaagaccg
      901 cccccgcaac tacccagtag agagcgctcg tccagcttcg catgttcggt ctcctcgcgc
      961 ggcgtgttcc catattctag cgcgagactc gaccgcgacc ccaaagacgc gtcgttccgc
     1021 taaactgcac gaccatgagc gaacgagaca ggagtcctct gtggggcggc aggttcggtt
     1081 cctctccggc cgaagctttc gagcgcctga acgcctccat cccgttcgac gggcggctcg
     1141 cgccctatga catccagggc tccatcgctc acgcgaagat gctcggcgag cgggggatag
     1201 tctcctcgga cgaggccgcc gagttggtgc gcgggctgaa gacggtgctg cgtgaggtcg
     1261 agacggggga gttctcgtgg acgctcgcgg atgaggacgt ccacacggcg gtggagcggc
     1321 ggctcagaga ggtcgcgggc gatgtggcgc tcaagcttca cacgggccgg agccgcaacg
     1381 atcaggtcgc tctggacctg cacctcttcg tccgggaggc ggcggaggac atccgtaccg
     1441 gcctgctcgc ggtgatgcgc gcgctcgtgg aagtcgcgga ggcacaccgg gatctcatcc
     1501 tgcccggcta cacgcacctg cagcgggcgc agccgatcct gctctcccac caccttctcg
     1561 cccacttcga ggcgctccgg cgggacctgg tgcgcttcga ggcggcgcgc gagtccgcga
     1621 acgtctctcc tctcggcgcg gcggcgctcg gcgggacgcc gcatcccgta ggcccggccc
     1681 tgacggctgc ggagcttggg atgcggccgt tgaccaactc gcttgacgcc gtctccgaca
     1741 gggacttcgc cctggacctg ctctacgcct gcgccgtgct cggggtgcat ctcagccggc
     1801 tcggggagga gtgggtgctc tggacgagcc aggagttcgc gttcgccacg ctcgacgacg
     1861 cctattcttc cggctcctca atcatgccgc agaaaaagaa ccccgacgcc tacgagctaa
     1921 tgcgcggcaa ggcggggcgt ctgatcgggt cgctaaacgc catgctgatc acgctcaagg
     1981 gcctacctct cgggtactcg aaggacctcc aggaggacaa ggagccgatc ctcgacgccg
     2041 tggataccgt actgtctaca ctcgccgtgt tgccggagat gctccgcacg gcgcgtttcg
     2101 acggcgagcg gatgctggcc gccgccgggg gattcgcgct cgcgacggag ctggcggact
     2161 tcctggcggc gcggggcgtg gccttccggg aggcgcaccg ggccgtcgga cgcctcgtgc
     2221 ggcggtgcga ggagctgggc gtgtcactgg aagacgtgcc gccggaggag cttgaggcgg
     2281 cgcatccggc gctggcggat ctgccgcgtg atctgttaac gccccgcggg agcgtcgcca
     2341 acaagcggtc cgccgggtcg acttcgccgg gatctgtcga aaaccagttg gagcgggcgc
     2401 gggggtttct ggagatgggc gggcaggccg gcagagagtc ttgagactag aataagctcc
     2461 ggctgatgtc gagaccggga ggcaaggcgt gaagatagtg cagatgtcgg acgttcatgt
     2521 gggctccggg ctttttcgac cggatctcct cgaagccact atcgaggaga ccaacgctct
     2581 ggagccggac ctggtggccg tggtgggcga cttgacgatg gagggctacc gctgggagtt
     2641 cgagggggcc agggcttact tcgaccgcct ggagtgcccg aacgtcgtct acgggatggg
     2701 caaccacgac gcccggaacg tcggctaccg gcacttcgag gagttcttcg gcatccggga
     2761 gaggtccgtg gagatctccg tccccgaagg tacggcgaag gtcgtctcgc tcgactccac
     2821 caagccggac ctggacgagg gcgaggtcgg ccgcgagcac tacaactggc tggactcgga
     2881 gttccggggc tgggaccggg gaccgagaat actcatgatc caccaccaca tcctggccgt
     2941 gcccggcacg gggcgcgacg tgaacaacct gcgcgacgcg ggcgacgtga tggctatact
     3001 gcgcgagctg aaaatagact tggtcctctc cgggcaccgg cacgtcccct atgtctggag
     3061 catctccggg gtgaggatcg tacactccgg caccgtctct acgatgaggg tccggggcgt
     3121 catgccgccg tcttacaacg tcatcgagtt cgatgaggag tcggtcagca tcaggctcca
     3181 ggagcccggc aagggggaga gcggcgagca gcccctcgcc agcttctccc gccagcccgt
     3241 ctccaccagc aggttctacc cggacttcga caacttcgtc gtcttcgacc gactgccgtt
     3301 cctcaaagac tagccgaagg gctgaccgga gctagaggta cttgccgcct tcttcgagct
     3361 gtcccgtctt ctcgaaggcc cggcgcagca gatcccggcc ctcttcacgc tcctcgtcgg
     3421 agcgggcctt ctcgaggaca gtctttacgg actcctcgat ggcgtcgggc tcgaacacga
     3481 tgtccggcag ggcgataacg acctcccgga tctggcggta gataaagtcc ggttctccgg
     3541 catacgccca tccgcggcgg gccaggaccc aggcccagcc cttcatccgg atggtgttct
     3601 cgatgtcctc gtcttcgagg ccgtacatct cgatccggag ccggtgctcg gtgatcaact
     3661 ccggccttat gcgtaccgtc gcgggcattg cgccagttta gcagccggga agcggccatg
     3721 aaaaagggag agccctgggg ccctcccaat aagtttctat gaagttggag tctgctagtc
     3781 caggataccg gcgtcctcca ggtgctgttt agccacgtcc tccgggtcct tctgctccag
     3841 gtcgacctct ccgttgagct tgcgcatctc cttggcgtcc aggctggcgg agacctcgtt
     3901 gaggatcttc tcggcgtccg ggttcttctt gaggaacttc tgcgtggtga cgggcgccgg
     3961 gtagtagaac ggccagatgg ccttggtgtc cttgaggacc gtcagcttcg gcgaggcgag
     4021 ctgtccgtcg gtggtgaacc cgatggctac atcggccttg ttctcggcga gagccttgta
     4081 tcggatgccg aggtcgttga ctaccttgat ctccttgaag tcgagtttcg ggtagttctt
     4141 ctgcatgttc gggtagccgt cggagcggtt ctggaactcg gagtaggacg agaagacgag
     4201 ctgcccggac ttgtccgcga ggtcgtcgag cgtcttgagg cccagcttct tggcgagcga
     4261 ggtcctgacc acgatgccgt agttatcgtc gaagggcgcc ggcgtgagca tcttcatcgg
     4321 cgtgtccttg cggttctcgt agaacttgct gacgatgtcg taggtctctt tctccgtatc
     4381 aggagccgtc ttcgggtcct tcttcaggat cgccacgaag gccgtgcccg tgtactcggg
     4441 gtagacgtct atggtgccgt ccttgaggga cttgtccatg acctgctcgg agccgaggtt
     4501 gagcttgcgc ttgacgtcgt agccggcgtt ctcgaaggcc tgagcgtaca tctcgccgag
     4561 gagtatctgc tcggtgaact tcttcgagct tacggtgacg ctcttgccgc taccgctatc
     4621 cgagctgccg ctactgcctc cgccgcaggc tgcgacgacg gataccagag cggcgacgag
     4681 aagcaccgtt accgcccgga tcaacatggt cttgttcttc actctttcct ccttgttcat
     4741 ttattttcca cgtctctgct gggctatctt gagccctttg ggagtaaagc cgctctccag
     4801 gcgtccgaag accagctccg tgacgaccgc cagaagcgcc acggagagag cgcccgccag
     4861 cgctatgggg gtgctgttcc gctggtagcc ctctactatg tagtcgccca gtccccccgc
     4921 tccgataaat gtcgccaggg tcgcgctcgc caccacctgc accgccgaag tgcggattcc
     4981 ggcgaaaatg atcggcgcgg ctatcgggat ctggatgccg gtgaggatct gccggcccga
     5041 gagacccatc cccttcgccg cgtccagaac ctgacggctt acctgtctca aacccacgac
     5101 cgcgttgatc aggatcgggg gtatggcgag cagcgtgagc gccacgagcg cggtagtgaa
     5161 gctgagcccg aatatcggca gcatcagcac aagtatggcg agcgatggta ccgcgcgccc
     5221 gatattgccc gtgttgatcg cgacggtgga gccgaggtcc gaccggttca cggcgatcgc
     5281 caggggcagg gacagtagta tcgcgaggag caccgccgct aacgagagct ctatgtgaac
     5341 gaagaagttc ttcgtgaagt ccggccgctg gagcacctcc atgaaagcat ccatctatcc
     5401 agccctcctc acccggcggg accaggggct aaatacacgc tccgtcctga ccagcacgag
     5461 gtccgcgagg atcgccagag cggtcgacag gaccgcgccg gttacgtaca tcgtggggaa
     5521 gtcgcgagtg atgccgtcga agaccaggtt gcccaggccg ccgccgccga tgtacgagcc
     5581 tatgaccgcg atacctatga cggtgacggt cgctatgcgg attccggcca cgatcaccgg
     5641 cagcgccagc ggcaactcga ctccgaagag gatctgacgg ttcgtgagcc ccatccctcg
     5701 cgccgcgtct atagtctccg ccgggacaga gtcaagacct gtcaccacgt ttcgtatcag
     5761 gatgagcagg gagtatgcga cgagagcgat gatcacgggc gtcctcccga cggacaaccc
     5821 gacgatgaag atcaaaatcg taaagaacga gaagctcggg atggtgtaca ggaagcccgc
     5881 gatagccgtg aggggcgcgt ataggtttcg cctgcgggag gccagcacgc ccagcggcag
     5941 cgcgatcacg agcgctatag ccaccgagat gaacgagagg aagacgtgct gcaagaacgc
     6001 cggcaatacg gtttcggtga ggtgatccgc gagccactcc cagtttataa agcccttctg
     6061 ttggaccgat ttgggggcga actcgctctg aaggaaggcc agtatggccg tcggagggtc
     6121 gtctgcgcta tccacgggac gcctccgcgc cgtgggagat cttccttatg gagtccaggg
     6181 tgatggtgcc gcgtgaggag ccgccgttct cgtctccgtc ctgcacgacg acgccgcggt
     6241 ccgctcccgc gccgatgatc tcggagagcg cgtcccgaag ggtccagccc gcgttgatgc
     6301 gcggcaggtc gtggccgttg ccgttcgccg gctccagctc gatgtcgccc acccgggtga
     6361 gcgagagccg cttgagcacc cggtcgcccc cgacgaacga ctggacgaag tcggaggccg
     6421 ggttgacgag gatgttctcc ggcgtgtcgt actgggcgag gatgccgcct tccttgagga
     6481 tcgcgatctt gtcgcccatc tttatcgcct cgtctatgtc gtgcgtgacg aagacgatgg
     6541 tcttcttgat gtctttctgg atgttgagga actcgttctg aagcctgtcg cgggtgatcg
     6601 ggtcaaccgc cccgaagggc tcgtccatga gcatgatcgg ggggtccgcc gcaagcgccc
     6661 gcgccacgcc gacacgctgc tgctggccgc cggagagctc cgcggggtag cggtcgcggt
     6721 actggtccgg agcgaggccg acgagatcta gtagcgtgtc tacccggctc gtcgtgcggc
     6781 tcttttccca gccaagcagg ttgggaacgg tcgcgatgtt gtcggcgatg gtacggtgtg
     6841 ggaagagacc tatctgctgg atggcgtagc cgatctttcg ccgcagttgc gtcccgctca
     6901 tggcggtgtt cttctgtcca tctatgagga tctcgccgct cgacggctct atgagccggt
     6961 tgaccatccg catgctcgtg gttttgccgc aaccggaagg tcccaccagg acacaaatct
     7021 ccccatcggg tatcgtgaag gagagatcct gtacgaccgg ccgctgcgag ccggcatacg
     7081 tcttacttac gtcattaaac tcaatcatgg tgtctccgca agatccaggg gtagaagtct
     7141 acccagtatg cctttctcgt ttcccttcct gcatccaaaa cgactccgtc tatcggcgac
     7201 aaaacaattg cataagtata gagcattctt cacagcaggg cacaaatctt cgtgctctgc
     7261 ggcacaagcg agctggagat ccgcgcccgc cggcggttgt cctcacagcg ccggtcggga
     7321 cctgatggac gcaggatact gaaggaggga tccacccggc ggtaacggcc agcctgtggt
     7381 tatccccggc gtccgattcc gagcagcagg tacaggacga agtaggccgc cgctgccgtc
     7441 agggctacga cgtagaagtt gcccgtatcc accgataact gcccggagga cgatatggcg
     7501 ttcaggatga tcgtcgcggg tgactccagc aactcgccca gactatatag ggcgtccgtc
     7561 aggccgttgg tggcgcgttc gacaccggcg taaaccatgc ccacgtgcgc caggatcacc
     7621 aggatcacga gtgcgagaat cgtacggatg agtcccatcg ctctagctcc tttgctcgaa
     7681 ggccgcagac tctcccatga gagtccagag acggcgcccc gaaaacgccg gagaagacca
     7741 ggatacgtcc cgtccgggag catagcgagg agcgcgcttc actacacgct tcgctgcgac
     7801 cttccctggc cgggctagag actcgattct ccccatgatc ctccctctcc gcgagcttcg
     7861 gagccatgca ccgcgccagt atactaagtc cggagttacg gcgcgcaaga atgcgccgtc
     7921 gaggtcaaga agttaagaag cttcccggcg gtagcctttg cctcgggtgc ggttctcccg
     7981 cgtcgtgcgc gtcgaagagg tgcgtttcgc gccgctgcgt cccgtagagc cgcgcttgcg
     8041 cttgacggag gggccactgg tcccgctctt gaggagcagc accgctccag cgatgaggaa
     8101 gcagatcatg ccgatggcga aagtcacgga gtcgcgcatg aagacgccga aagccgcggc
     8161 gacgcccggc acccaccaga tcgcgaagag cttgccgggt tcgcgagtct tggggtgcgt
     8221 ggacatgtat atgccgaacg cgacccccat gagggatatg accaggatca gcgcgcctat
     8281 gaagataatg attaagctca cgcgactata ctaaccgcga gcccgaggtt taacaaagta
     8341 ttcatcgcac gcgcaactac cggcggctca acgcgcggcg gtaggacttc atggcggaca
     8401 ggaactcatc ccacgagcgc agcggagact ccaggcggtt gttgtagaga gagaacttgt
     8461 ggaccagctc gtgcagggcg gaggcgtcgt cggagacgca ccgggggtcg gccacgatca
     8521 ggatctccca gtcctcctgc tcctttatat tatctatggc cgccttgagg ccgggtttat
     8581 ggtagaggag tttccctggg gcctgcgagt ccgagtagat ctctaccagg acgtacctgt
     8641 tggcggcggc gtagtcttct attgcggaac gttgctcctc ctgggcgggg aggccgcctt
     8701 cgggatcggt ccggatgtag gctgcggcgc gcgtcacgaa acccgaatga tagcatgcgt
     8761 cccgtctgat attatgctgg tgaactttgc gtcgggacgt ggcgcagtct ggtagcgcac
     8821 tcggctgggg gccgagtggt cgccggttca aatccggccg tcccgacttc tcacggaaga
     8881 cccgccgcac ggaaaggctt gctccgactt ggacactatc tatcttctac tcggaatcat
     8941 agggttcatg gccctgatcg cgctgttctt cttcggcggg ttcgtcctgc tggactttat
     9001 ctggcagcgg cgcgggggag acctctaggg ttttcgaggc ccggcccagg cgttccagcc
     9061 tcctttgccg cgcgtgatct cttcgctcga agacctgcgc gtcgttcctg tggaccgcct
     9121 gatcctgcac gaggctcacg acgagcgccg tctcgccagc ctgcgcgagc gcattacctc
     9181 cgaaggcgtg cagcgaaacc cggtcatcgt ggcgccccat gaatcggatt atctggtcct
     9241 cgacggcgcc catcgcgttc acacaatgcg cgagatcggt tgccgcctca tcctggtcca
     9301 gatagtcgaa cctccctcgg cggccgagag ctggggacat ctcctcaccg gcatggacat
     9361 cccggaattg cggagcgtgt cagaggtggc ggtcagggag gagcctggcg gcccgtccct
     9421 cgccactgtc gagatggccg gaggagagcc cgtgtacatc gaggcgcgcg ggggggacgg
     9481 gctgcgggag gatatacggg ccctgtgggc cattcatgcg ctgtatcccg atggagacgt
     9541 ggtgcgccgc gtggaccctg atgggccggt ggagatcggc gcgggagagg cgctcgtccg
     9601 gtaccggact ttcactgtcg aggaactggt ggaggtggtg cattgcggaa cggtgcttcc
     9661 cgccgggatc acgcgcttcc gcttccgcga gcggatcctc ggcgtgcgtt tcccgctgga
     9721 gcacatgcgg gacggggatg tcgaggcggg caactccgaa ctccggcggt tcgtaaacga
     9781 gcactggcag cagaaccgga tccgttacta tgacgagccc gtggtcctct tcgagtaacc
     9841 cggccggtaa ctcgcgtcag acgcgcggcg ggagagcgat ttctagtcta tgcttgtggc
     9901 ggggtgagag caggcgcgga acagaggagt acgtcttgag tggatcggat gtaacgaagg
     9961 ccgtgatgag gggcgagatc agccgccgga ggttcctgaa gctggggggc gcggggctcg
    10021 cgggggtggc gatgctcggg gcggccggtt gcggcggcga ctccgagcag ggtagcggcg
    10081 ggacgaccag gctcactttc accttcgggc cggatgcgtc cggcgggctc cagactctca
    10141 tagaccgctt caacaagcag aacaagggtg agatcgaggt caactggcgc gagacgccgg
    10201 ccgcgagcga cgagtacttc gagcaggtgc agtccgagct gcaatcgggg cagtcgaaga
    10261 tagacgtggt cggcggcgat gtcgtctggc cggcgcagtt cgccgccaac ggatacctgc
    10321 tcgacctctc cgaccgcttc accagcgaga tgcaggcgaa gtacctgagc ggcccgctgg
    10381 agccgatcaa gtacgaggga aagacctacg cggtcccctg gttcaccgac gcggggatgt
    10441 tctactaccg gaaggacctg ctggagaaga gcgggttctc cgagccgccc gttacctggg
    10501 acgagatgaa ggacatgatc gagaagatcc ggtcggactc cggcacgaag tacgggtacg
    10561 tcttccaggg ggcgcaggac gagggcggcg tggtggatgc gctggagcac atctggaacg
    10621 ccgggggcag cgtgctggag ggcaacaagg tcgtcgtaaa cagccccgag gcgcgcaagg
    10681 gtctcgaagt ccggcggtcc atgatcacgg gcggcttctc tccgagggcg agcggcgact
    10741 atagcactca ggagtcgcag gccgtcttta ccgacggcaa cgtcatcttc atgcggaact
    10801 ggccgtttgt ctacgggctg ctctccaacc ccgacgactc caaggtgcgt cccgagcagg
    10861 ttgacatagc gcccatcccg gtcagcgaga agggggcccg aagcttcagc ggtctcggcg
    10921 ggtggaactt catggtcaac gcgaactccg aggacaagct cgatgagatc tggaagttca
    10981 tccagttcat gacctcgccg gagtcgcaga agacgctcgc cctgcagagc acgcgcctgc
    11041 caaccctcaa gagcctgtat gaggaccagg agcttctcga caaggtgccg gtggccgcgc
    11101 gcggcaagga agccctgcag agcacccggc cccggccgat ctcgccgtat tactccgaca
    11161 tgtcgctcaa gatggccgac agtttcaacg ctgctctaaa aggcgaggtg cccgtcgcga
    11221 gcgccctcgc caagctgcag agagatctgc agaacatcgt ggatcagggc tagagtcggg
    11281 cgccgccgta gcgtaacgcc gcccgccgat tcaagagatt caaaagcaaa accctttgtg
    11341 ctacgctgca accgtggcag agagatccgg ctacccgcgt gagactaagg gtgccgcctc
    11401 ggcctactcg aattacctga gcatcctgtt cagcccgctg ttgttcgctc caggaagggt
    11461 tctatcatcg ctgtggaggt aaaagtttga cggctacggt cgtcctcggg ctagcctggg
    11521 gagatgaagg taagggacgg gtctgcgacg ctcttgcgac cgatgcggat tacgtgtccc
    11581 gctactccgg cggcaacaac gccggacaca cgatccgggt cggcaaagaa gagttcgtgg
    11641 tgcatctgat cccgtccggg atcgtacgcg agggggtcat ctgtacggtc ggcaacggtg
    11701 tcgtcgtgaa cccggacgtg ctggcggagg aggtcacggc gctggaagga cggggcatca
    11761 gcgtgcgcga ccggctcaag atagacggcc gggcgcacct gatcatgccg taccacatcc
    11821 ggctcgactc ccaccgggag aaggccctcg ggaaggcgaa gatcgggacg acgaaccgcg
    11881 gtatcggccc gacctacgag gacaaggtgg cgcgcgccgg catccgcgtc caggacatct
    11941 tcgacgaggg catcctgcgg acgaagctga aggccgccct gcgcgagaag aacgccatct
    12001 ttaccagcat ctacgacgag gagcctttcg acccggacga gctggccgac ggtctgctcc
    12061 ggcaccgggg tctcctggag ccgatggtcg ccgacacggg cacgatgctg cgcgacgcgc
    12121 tggaccgcgg ggagaaggtg ctgctcgaag gcgcgcaggc gacgctcctg gacaacgact
    12181 tcgggacgta cccgttcgtt acctcgtcca acccgtcggt cggcggggtg tgcgtcgggg
    12241 cggggatacc gccgcgggag ctggagcacg tgatcggggt gacgaaggct tacacgacca
    12301 gggtcggcga cggaccgttc ccgaccgagc tacgggacga gaccggagac attatccggg
    12361 aggccggtca cgagtacggg agcaccacgg gtaggccgcg gcgttgcggg tggctggacc
    12421 tggtggccgt caagtggacg gcgcggttga acgggatcac gcatctcgcg gtcacgctgc
    12481 tggacgtgct ctcggcggtg gaggagatca acatctgcgt cggctacgag atcgacggcg
    12541 agcgcgtcga gggcttcccc atgagccaga cggatctcca ccacgcgaaa cccgtctaca
    12601 agactctgcc aggctggggc gtggacatca cgggatgccg gatgcgcggg gacctgccgg
    12661 aggcggcgcg ggacttcgtt gagttcgtgg aggctgagat cggggctccc ctgtgcatga
    12721 tcagcgtcgg accggagagg gatcaggcga taatcgagag gataggggcg taaagagatg
    12781 cgggtgatgg tcgtcgggag cggcgggcgc gagcacgcgc tggtcgagac cctcgccgcc
    12841 agccacatcg aacccgagat gttcgcggcg ccgggcaacc ccggcatcgc ccgtatcgcc
    12901 gaggtcgtgg acatcccggc cgacgacctt atcggtctgc gggacttcgc cagcgagaac
    12961 cggatagacc tgaccgtcgt cggtcccgaa gtgcccctga tcggtggcat agcggagtgt
    13021 ttctgggagg aggacctgcg gatcttcggc ccttcccgcg ccgccgcccg tatcgagggc
    13081 tcgaaggtct tcgccaagga gctcatgaag catgccggcg tacccaccgc cgatttcgag
    13141 gcgttcgacc gggagggctc ggcgctcgcc tacctgcggg ctcacagcga ttatccggtg
    13201 gtcgtgaagg ccgacggcat cgcggcgggc aagggcgtga tgatagcgcg ctcgcgggaa
    13261 gaggcggagg aggcggtgcg cgaggtcttc tccgggaagt tcggggcggc tggcgagcgc
    13321 gtcctgatcg aggagtgcct ggaggggcgc gaggcgtcag tcttcgtgat gacggacggg
    13381 gtgaacatcc tgccttttct accggcccag gactacaagc ggatcgggga cggggacgag
    13441 ggaccgaaca cgggtgggat gggcgcgtac tcgccgctca tgtggatgga gcccgccacc
    13501 tacgcggcga tactggagga gatcatccgc cccacgcttc accagctcag cctgatcggg
    13561 gctccctact cagggcttct gtacgccggg gtgatggtga cggagaccgg cccgaaggcc
    13621 ctggagttca actgccggtt cggcgacccg gagacgcagg tgatactgcc gcgcctggag
    13681 acggacctgc tggagctgat gctcgccgtg aacgacgaga cccttgccgg gcgcgaggtg
    13741 gagtggctgg cggagaaggc cgtgtgcgtc gtgctcgcgt cggagggata cccggagact
    13801 tctcactcgg gggacgagat cggcggcctg gagaacatcc ccgccggggt ctacatctac
    13861 cacgcggcca ccgaggagcg cgacggcaag ttctacaccg caggcgggcg ggtgctgaac
    13921 gtcgtcggca ccggacccac ggtgatagag gcgcgggcac gggcgtacgc ggcggtcgag
    13981 cagatccact tcgcgggcat gcagtaccgc accgacatcg ctctcgaagc gatagagctg
    14041 gaggacattt gaactccgtc gatggccgcg cgagatcaag ccgccctggc gccgggagcg
    14101 ctccgtgatc gaacgctaca ccctcccgga gataggcgct gtctggaccg aggaggcgaa
    14161 gtaccgtgcc tggctgaaag tcgagatcgc cgtctgccgg gcgagggcgc aactcgggga
    14221 gatcccctcg gacgaggtcg aggaactcgc ggagaaggcg gacttcaccg tcgagcgcat
    14281 ccacgagatc gaggccgaga cgaaccacga cgtcatagcc ttcgtctcca ccgtcgccga
    14341 gtatgctggt gacgtgtccc gccacttcca cttcggcctg acgagttcag acgtgctgga
    14401 cactgcgggg gcgctgcaac tgcgggacgc cctggacttg atcctcacgg agacccgcgg
    14461 gctgacccgt cttctcgcgg gcatgtctct cgaacaccgc gatacggtga tggtgggacg
    14521 gacgcacggt gtccacgcgg agccgacgac gctgggtcac aaggtcgcgg tgtgggcgtt
    14581 cgagatggag cggaacctgg atcggctatc tcatgccagg gaggtcgcag cggtcggcaa
    14641 gataagcggc gcggtaggca cctacgcgaa cgtggacccg aaggtggagg cgctgacgtg
    14701 cgaggagctt gggttgtctt cttcgcccgc gtccacccag atcgtgcagc gcgaccggca
    14761 cgcggaggtc ctggcgtcgc tggctatcct ggcctcgacg ctggagaaga tcgcgctgga
    14821 gataagagga gcgcagcgga ccgagatccg ggagcttgcc gaacctttcg ggcgtggcca
    14881 gaagggctcc tcggcgatgc cccacaagaa gaatcccatc ctgaccgaac ggttgtgcgg
    14941 catggcacgc ctgatgcgcg gatacgcggc ggtcggcttc gagaacaacg ccctctggca
    15001 ggagcgcgac atctcgcact cctccgccga acgagtcgtc ctgccggacg cgacgatcct
    15061 ggccctgtac atgctacgga ccaccgggcg cgtcctgcgc ggccttcagg tccacgagga
    15121 ccggatgcta cagaacatga acctcggcgg cggcatcgtg ttctccgggc gggtgctgct
    15181 cgcgctggtc gaggcgggca tgagccgcga cgacgcctac aaggtagtgc agggcgcggc
    15241 gatgcgggcg tgggagggcg agggcggctt ccgggagctt cttgaggaga gcgatgaggt
    15301 gcgcgagcgg ctgggggagg acctggacgg gctcttcgat ccctcctacg cgctgcggaa
    15361 cctgggggtc gtgttcgagc gggtggagga gctgaaggag aggttgggcg atggctgagg
    15421 acttgctcta cgagggcaag gcgaagaagg tcttcgccac ggacga
//
LOCUS       DASSMB010000142        14942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_793056, whole genome shotgun sequence.
ACCESSION   DASSMB010000142 DASSMB010000000
VERSION     DASSMB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14942
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793056"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="VFJ72_08525"
     CDS             <1..885
                     /locus_tag="VFJ72_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7479541.1"
                     /translation="RSLLAVPATNLKMVQKGLASSADGVFLDLEDAVAPDRKEDARND
                     VIHAIKEMDWRTRPTLFRANALDTPYFYRDLIEVVEEAGDYLDRILIPKVQRHEDLYT
                     VHTLLSQLELATGLEPARIKLEAQIESAEGLTNIDAIARATSRLEALHFGPGDFAASL
                     HMPQTSIGTMDEWDEAYPGHRFHYAMQRIVVACRAAGLRAVDGPVADYRDEEGLRKAC
                     LLARSLGYDGKWCIHPAQIGVVNEVFSPAEREIEWAKKVVAAYEEASAAGSGAVSVEG
                     RMVDAASIKMARNTLDLT"
     gene            1097..2998
                     /locus_tag="VFJ72_08530"
     CDS             1097..2998
                     /locus_tag="VFJ72_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479542.1"
                     /translation="MIEALKESEEQFAIIVQNALDIVMITEADGSIRYITPSVERILG
                     YMSEEMVGTNIAEYVHPEDLEMALRELGEAVSRPGVYPVAVETRVRRKDGSWRYLEGI
                     ANNLLGDPDVRGMVFNHRDVTDRVRAEAEVRRLNEELEDRVAERTAQLEAALAERERT
                     QRELKRSRDELEVILRGVADGITAQDSAGRLIYANDAAAKIMGYPSARDLLQALPAEV
                     MRNLEIRDEAGNPYPLERLPGRTAIRTRQNVETILRFRQVETGEERWTVVRSAPVLDE
                     RGDSRFEVNIFHDITDRERADIERRQLSAIVESSDDAIISKTLDGTITSWNSGAQKIY
                     GYSAEETVGMPISMLVPDDRPNEIPGILERLSRGEKIDHYETVRVTKDGRRLDISVTI
                     SPIRNSAGRIVGASAIARDITERKQAEDAMRRIREAERHRIARDLHDSVLQDIAYTLQ
                     TLEVTKMKAEGTDLEEELVEEVGAMRTAARGLRNAVYELRLGDERNRPLVRLVKDLVE
                     QSRQRSPGCEIELDLRPEVSVTPLGDNGTELLRIIQEALTNVRRHSGARRVLVRLRVD
                     GEYLVAEVVDDGHGFGPEVDAGVGTRSMNERAAALGGELRVESEPGRGTRVRLRVAMS
                     ALLQGQRAG"
     gene            3097..3483
                     /locus_tag="VFJ72_08535"
     CDS             3097..3483
                     /locus_tag="VFJ72_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479543.1"
                     /translation="MQDGDLTKTVRVMLVEDHAAFRRGLAFILDREPELEVVAQAGSL
                     AEAREHAGSRSFDVAVTDLGLPDGTGTDLILALREINPDVEILVLSASLDWQNLAAAR
                     EAGAGEVLDKFATPGEIVGAVRRLGA"
     gene            3496..4248
                     /locus_tag="VFJ72_08540"
     CDS             3496..4248
                     /locus_tag="VFJ72_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET7479544.1"
                     /translation="MNRSGGLSPVPPPVRIDARFIRWLRLALFVVSAAAFSLLYLLNA
                     EFRSETNLALGILGRGDIAGLRDYIVSSFGFWAPVASLFLMVLQALVAPVPSFLITFA
                     NGLAFGVFWGWMLSLAGHVLAAAMCFGISRSLGRVPVEVLVGKTGLESADRWFARWGM
                     YAIFVARLIPGVAFDAVSYAAGLTRMRFRNFIVASALGILPQTFLYSYLGRQAPEYVA
                     LFLVTSGLVVAAVVAAAVVRSKRRRKPSGRVR"
     gene            4299..4931
                     /locus_tag="VFJ72_08545"
     CDS             4299..4931
                     /locus_tag="VFJ72_08545"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET7479545.1"
                     /translation="MSRMLEPSYDLAGRYSGTLVVITGSMFSGKTEELIRRSRRALYA
                     RRSVQVFKHALETRPEGEEIRSHNGIPHEAIAVSTSGELLARVEPATDVVAIEEAQFF
                     DAGIVEACRRLADAGYEVIATGLDMDFRGEPFGPMPALMAGSDEVVKLRAICARCGRD
                     ASRSQRLIDGRPAPATAPTILVGAEESYEARCRHCHEVPRTIFQHGLPGV"
     gene            4932..5363
                     /locus_tag="VFJ72_08550"
     CDS             4932..5363
                     /locus_tag="VFJ72_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479546.1"
                     /translation="MPRRWVIALLLFCIVLNAAGFMWKLFEPVPLYDEVAHFLTPFTL
                     VAITAEIIYRSGGDDEFFDTPRRAVLTGLVIGFAGAVGWEGVEALLAAMGFSISNALP
                     DTIFDVFLGVAGGAAGAWAADRYLDRIFKRSRTNARRPRVR"
     gene            complement(5360..6511)
                     /locus_tag="VFJ72_08555"
     CDS             complement(5360..6511)
                     /locus_tag="VFJ72_08555"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET7479547.1"
                     /translation="MSETVLPGSTVGVLGSGQLGRMFALAARRMGYRVHVYSPDTDSP
                     AGQGADREWSAPYEDLDAAREFAGSVEVVTLEFENIPASVVEEIEALAPVRPGIRALH
                     TAQHRLREKTFLSEAGFPVTPYRTVGSASELRTALEEIGTPAVLKAAGFGYDGKGQVK
                     IEAPNEAAAAWETLGENEAVLEEWVDLQGEVSVVGARGLDGSFAHYGAVENTHRDHIL
                     DLTVAPADLPPETSKEAVEVSRGVFEALDVVGTACVEFFVAANGKLLVNEIAPRPHNS
                     GHWTIEGAVTSQFEQQLRAVCGLPLGSTHQVRPAAMANLLGDLWRGGEPDWAKALAMP
                     EVRLHLYGKAEARPGRKMGHLTSLAGDPREAARRVLAAREALPPARRAG"
     gene            complement(6508..7044)
                     /gene="purE"
                     /locus_tag="VFJ72_08560"
     CDS             complement(6508..7044)
                     /gene="purE"
                     /locus_tag="VFJ72_08560"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET7479548.1"
                     /translation="MDRRESSARSSGEAGLESNEALVGVIMGSTSDWETMRHAVEALR
                     DFGVPHERRVVSAHRTPRLMSRYAEEAEARGIEVIIAGAGGAAHLPGMVAAQTALPVL
                     GVPVESRTLKGVDSLLSIAQMPAGIPVGTLAIGKAGATNAALLAVAILANSRPELRER
                     LHVFRQEQTRKVLEAELP"
     gene            complement(7409..7786)
                     /locus_tag="VFJ72_08565"
     CDS             complement(7409..7786)
                     /locus_tag="VFJ72_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479549.1"
                     /translation="MASARTPYPRTDFWLIVAEKDGRVLAVDGDGTPAIFSGEGEAEM
                     FRYLLGLDEGGWMVRRTSPGDLISVLYCHCADANKVALDPAPEMLSARLIGLVRMSKV
                     RFIARILERVLERGRLLGNPAVS"
     gene            8176..9057
                     /locus_tag="VFJ72_08570"
     CDS             8176..9057
                     /locus_tag="VFJ72_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET7479550.1"
                     /translation="MATRAVIIGNPNSGSAGDEGYLERFAETLRAGGLEVEVLNTERP
                     DHATELAAAAGDRLVVAAGGDGTVNEVVNGLSEGATLGILPLGTANVLARELGLPLEP
                     EDACKRILTGTGARMDVGVATDEEGTERRFTCMAGIGFDANVVREVTPRFKRYLRSLA
                     FPLAALKVYFRGDQPDLQIVDGNTTYVTQFAVVANGQYYGGEFEMAEDASLGSGALEV
                     VLIEKVGLLLRPDVLARILAKSPLDRAMKSFSSRGILAKSPAARGARVPVQIDGEVWG
                     RLPMSFRIEPAAIEVIR"
     gene            complement(9131..10201)
                     /locus_tag="VFJ72_08575"
     CDS             complement(9131..10201)
                     /locus_tag="VFJ72_08575"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET7479551.1"
                     /translation="MNLDRPYPDEAPGETRRSATRSRTNQYAELKRLIKQEGLLDQQP
                     AYFAGKSILTFGLLAVSLTLLLISGNTWIQLLNAAYLAFVFVQISLLAHDSGHRQISF
                     RAPWKNDLLTLVLGNFLLGMSRQWWIDKHNEHHGHPNQIDVDPDIDIPLLAFEEEQAL
                     DKRGIARFVVKYQAVLIFPLSLLQALSMLRSSITFLMARKANNALAEVLAMGAHFVLY
                     FVLLFSTLEPLQAVLFIIVHRGLYGLYMVSIFAPNHKAMPLLEQDSEEDFLRRQVLTS
                     RNVFAHPVTDFWYGGLNYQIEHHLFPRMPRNKLREAQPIIKDFCRVHSIAYHETSVLQ
                     SYREILQHLHEVGAPLRKGTAV"
     gene            10599..11297
                     /locus_tag="VFJ72_08580"
     CDS             10599..11297
                     /locus_tag="VFJ72_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479552.1"
                     /translation="MDERSKIGAIRVLLADDHTMFRQGIAGVLEEYGGMEVVGQTNND
                     ERAVELARRTRPDVVLMQAQMPIQRARENLRRMRENVEPPPKVVIVTMIEEPRRVREI
                     MNLGVSAYIVKSASAEHLVAAVRAAILDPKSKDVVVGMPLDLLEEAEKGSRGVLSVRE
                     LEILLLAARGLSNRQIANSLHVAEGTVKRHLANTFEKMNVRSRGEATRMALHEEWITI
                     QDVTEDLEEPEESG"
     gene            complement(11294..11659)
                     /locus_tag="VFJ72_08585"
     CDS             complement(11294..11659)
                     /locus_tag="VFJ72_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479553.1"
                     /translation="MRILIANTPRMYRESLALSIQQRRPHYEVLISDPKTLDGQVERL
                     TPRVLVRDDDGVEAGAPDGVVCWVGIIIDNHLNARISVDGRISEIHDVSLEEFLAALD
                     ETEKLISADGGPSEPSPEV"
     gene            12126..12329
                     /locus_tag="VFJ72_08590"
     CDS             12126..12329
                     /locus_tag="VFJ72_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479554.1"
                     /translation="MNHALLIALDERANTLMDVVFTLDRLAEAPDLRADELRLRRLKH
                     QIEEAEYQLRISRMELDLARMRM"
     gene            complement(12392..13378)
                     /locus_tag="VFJ72_08595"
     CDS             complement(12392..13378)
                     /locus_tag="VFJ72_08595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479555.1"
                     /translation="MSRILLVDDHAWFRQAFGMVLGWETDFRVSTQAGSLAEARACLS
                     ADSLSADEAEEIDAAIIDLALPDGDGTDLIAEIGALREVTRRIPVMVLTATENPEIHA
                     RLRELGAVEVLSKSAFLEEILAAARRLGGKVKAMHKKQLVSDMVSGVLSRQAMVLSER
                     TGEPFGVAMHAVLQTEAGRQLGKLRDGPHCDERAEQWQENLAPKRAKERRRTRREEHS
                     RVLQEASWQLFMQSEMRDLELRKDGQLARVLDRMRGTTPAVPAVLRLVSEDQRQAEEG
                     LVALTSGGKVSYKRLDDLTPEDRPARIASNRLRTTWLKQNQDGWLNHSEERL"
     gene            complement(13375..13611)
                     /locus_tag="VFJ72_08600"
     CDS             complement(13375..13611)
                     /locus_tag="VFJ72_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479556.1"
                     /translation="MTEATTKRLGPCQVRSKTEHPCHNRAVVKIYGIPFCKACAREQE
                     AYFAIGELTQEAQDLGSEPLVGAGYRVRREHVGI"
     gene            complement(14126..14842)
                     /locus_tag="VFJ72_08605"
     CDS             complement(14126..14842)
                     /locus_tag="VFJ72_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479557.1"
                     /translation="MAVNGKESKMKITASNLIRWAGLSAMGAGIIFAGIQPIHPPDVV
                     SSVTTGAWAIITPLKTVMCLLLLLGIAGIYARQVREAGWLGLAGFLLFGLGWALITAF
                     VFAETFIMPPLASVAPKFVDGFLGVASSRASEVGLGAIPALFALAGILYMLGGLLFGI
                     ATFRAGILPRWAAGLLAVTAALTPLAALLPHEIQRLAAMPMGLALACLGYALWSERRE
                     QASEPLPDKGSPQLRQTAAE"
BASE COUNT         2697 a         4707 c         4880 g         2658 t
ORIGIN      
        1 cgctccctcc tggccgtccc ggccacaaac ctcaagatgg tccagaaggg actggcctca
       61 agcgccgacg gcgtcttcct cgacctcgaa gacgccgtcg ccccggacag gaaagaggat
      121 gcccgaaacg acgtcatcca cgccatcaag gagatggact ggcgcacccg acctaccctc
      181 ttccgggcca acgccctcga caccccctac ttctaccgcg acctgatcga ggtcgtagag
      241 gaagccggag attatctcga ccgcatcctc atccccaagg tccagcgcca cgaagacctc
      301 tacacggtcc acacgctcct ctcccagcta gagctcgcca ccggcctcga acccgccagg
      361 ataaagctcg aagcccagat agagagcgcc gagggcctca ccaacataga cgccatagcc
      421 cgcgcaacca gccgcctcga agccctccac ttcggacccg gagacttcgc cgcgagcctg
      481 cacatgccgc agactagcat cggcaccatg gatgagtggg acgaggcgta ccccggtcac
      541 cgcttccact acgccatgca gcgcatagta gtcgcctgcc gcgccgcggg gcttcgggcc
      601 gtggacggcc ctgtggccga ctaccgcgac gaagaagggc tgcggaaggc atgcctcctg
      661 gcccgaagcc tcggctacga cggcaagtgg tgcatccacc cggcgcagat aggagtggtc
      721 aacgaagtct tctcgccggc ggagcgggag atagagtggg cgaagaaggt ggtggcagca
      781 tacgaggagg cgagcgcggc cggcagtggg gcggtcagtg tggaagggcg gatggtggat
      841 gcggcatcca taaagatggc gcgtaacacc ctggatctca cctagtttcg gggctcacga
      901 tccataaccg cgtctcaggc gtatagatga tcgtgtggtt ctgtgaaagg gtcttccgca
      961 ctccgcggct acgcaggggg tggcctggat gcttttacgt tacgtaattc ccgtggtttc
     1021 cggtcgagcg gcactgggct agaattgtcg aagccgatgg gttggctgga aggagaagat
     1081 cgtcgacaac agcaggttga tcgaggctct caaagagagc gaagagcagt tcgcgatcat
     1141 cgtccagaac gcgctggata ttgtcatgat caccgaggcc gatgggtcca tccgctacat
     1201 aaccccctcg gtcgagcgga tactgggtta catgtccgag gagatggtag gcaccaacat
     1261 cgcggagtac gtccatccgg aagacctgga gatggccctc cgggagttgg gcgaggccgt
     1321 ctcaagaccg ggggtgtacc cggtggcggt ggagactcgc gttcggcgca aggacggctc
     1381 ctggcggtac ctcgaaggga tagcgaacaa cctgctcggc gacccggatg tgagaggcat
     1441 ggtcttcaac caccgggacg tcacggaccg ggtgcgcgcg gaggcggagg tgcggcggct
     1501 caacgaggag ctcgaagacc gggtcgccga gcgcaccgcg cagctcgaag ctgctctggc
     1561 ggagcgcgag cggacccaga gagagctgaa aagatcccgg gacgaactag aggtcatcct
     1621 gaggggggtg gccgacggca tcaccgccca ggactcggcg gggcgtctga tctacgccaa
     1681 cgacgccgcc gcgaagatca tgggttaccc gtcggctcgc gaccttctgc aggcgctccc
     1741 ggctgaggtg atgcggaacc tcgaaatacg ggacgaggcg gggaacccgt accctctgga
     1801 gaggcttccc ggacgaacgg ccattcggac cagacagaac gtggagacga tactgcgttt
     1861 ccggcaggtg gagacgggcg aggaacgctg gaccgtcgtc aggtcggcgc cggtcctgga
     1921 cgagcgggga gactcgcggt tcgaggtcaa catcttccac gacataaccg atcgggagcg
     1981 ggcggatatc gagcgcagac aactctccgc catcgtggag tcctcggacg acgccataat
     2041 cagcaagacc ctcgacggca cgataacgag ctggaacagt ggagcacaga agatctacgg
     2101 ctactccgcg gaggagaccg tgggcatgcc gatctcgatg ctggtccccg acgaccgtcc
     2161 caatgagata ccggggatac tggagaggct cagtcgtgga gagaagatcg accactacga
     2221 gaccgtgcgg gtgaccaaag acggtcggag gctggacatc tcggtcacca tctcgcccat
     2281 caggaactcg gcgggccgca tagtgggcgc ctccgccatc gcgcgcgaca tcacggagcg
     2341 caagcaggcc gaggatgcca tgcggcgcat cagggaagcc gagcgccacc ggatagctcg
     2401 tgatctgcac gacagcgtac tgcaagacat agcgtatacc ttgcagaccc tggaggtcac
     2461 aaagatgaag gccgagggta cggacctcga agaagaactg gtcgaagagg tcggcgcgat
     2521 gcggacggcc gcccggggtc tgcgcaacgc tgtctacgag ctgcgtctcg gagacgagcg
     2581 aaacaggccc cttgtgcgcc tcgtgaagga cttggtggag cagagccgtc agaggtctcc
     2641 ggggtgcgag atagagctgg acctgagacc ggaggtctcg gtgacgccgc ttggagacaa
     2701 cgggaccgag ttgctgcgga taatccagga ggcgctgacg aacgtccggc gtcactcggg
     2761 agcgcggcgc gtcctcgtga gattgagggt ggatggcgag tacctggtgg ccgaggtggt
     2821 cgacgacggg cacgggttcg ggccggaggt ggatgccggc gtggggacga ggagcatgaa
     2881 cgaacgggcg gcggcgctcg gcggggagtt gagagtggag agcgagccgg gcaggggcac
     2941 gcgggtacgg ctgcgggtcg cgatgtccgc tctgctccag gggcagcggg cgggatagac
     3001 cgggccgggc agtagcaggg gcgcgggcgg ccgcacaacc gtatgataac ggggtatgcc
     3061 ctgtggcata ctggcggcgt cgcggaaagg acagtagtgc aggacggaga ccttacgaag
     3121 acggttcgcg taatgctggt ggaggatcac gccgccttcc gccggggtct cgctttcatc
     3181 ctggaccgtg agccggagct cgaggtggtg gcccaggcgg ggtctctcgc cgaggcccgc
     3241 gagcacgccg gctccagatc attcgacgtg gcggtgacgg atctcggcct ccccgatggg
     3301 acgggtactg acctgatcct ggcgctgcgc gagatcaacc cggatgtgga gatactggtg
     3361 ctgagcgcga gcctcgactg gcagaacctg gcggcggcga gagaggcggg cgcgggggag
     3421 gtgctggaca agttcgcgac acccggcgag atcgtcggcg cggtgcggcg tctgggcgcg
     3481 tagagcccgg cccggatgaa caggtctgga ggtctctcgc ccgtccctcc gccggtcagg
     3541 atcgacgccc gattcatccg ctggttgcgg ctcgcgctgt ttgtcgtatc cgcggccgcg
     3601 ttctcgcttc tctacctcct caatgcggag ttccggtcgg agacgaacct ggccctcggc
     3661 atcctgggac gcggggacat agccggcctg cgggactaca tcgtctcctc gttcgggttc
     3721 tgggcgccgg tggcctcgct gttcctcatg gtcctccagg cgctcgtggc gccggttccg
     3781 tctttcctca taaccttcgc caacgggctg gctttcggtg tcttctgggg ctggatgctg
     3841 agcctcgccg ggcacgtcct ggcggcggcg atgtgcttcg ggatatcacg ttccctcgga
     3901 cgggtgccgg tggaggtgct ggtcgggaag acggggctcg agtccgccga ccgctggttc
     3961 gcccggtggg ggatgtacgc gatcttcgtc gcccggctca taccgggcgt cgccttcgac
     4021 gcggtctcct acgccgccgg tctcacccgg atgcggttca ggaacttcat cgtcgcgtca
     4081 gcgctcggca tacttccgca gacctttctg tactcctacc tcggacggca ggcgccggag
     4141 tacgtcgcgc tgttcctggt gacgtccggg ctcgtggtcg cggcggtcgt ggcggctgcc
     4201 gtggtgagga gcaagcggcg gcggaaaccg tcggggcggg tgcgttagtc ctgagcggta
     4261 gcctgaaccc tccgcgaggg gcggatagaa tgcggggcat gtcgcggatg ctcgaacctt
     4321 cgtatgacct ggcgggccgg tattcgggga cgctcgtcgt gatcaccggc tccatgttct
     4381 cggggaagac ggaggagttg atccggcgct cccgccgcgc cctctacgcc cgtcgcagcg
     4441 tccaggtctt caagcacgcc ctggagacgc gcccggaagg cgaggagata aggtcccaca
     4501 acggcatccc gcacgaggcc atcgccgtct cgacgagcgg cgagctgctc gccagggtcg
     4561 agcccgcgac cgacgtggta gccatcgagg aggcgcagtt cttcgacgcc ggtatcgtgg
     4621 aggcgtgccg tcgtctcgcc gacgcgggct acgaggtgat cgccaccggc ctcgacatgg
     4681 actttcgggg ggagcccttc ggaccgatgc ccgctctcat ggccgggtcc gacgaggtgg
     4741 tgaagctccg cgccatctgc gcccgctgcg gccgggacgc ctcgcgctcc caacggctca
     4801 tagacgggag gcccgctccc gcgacagcgc cgacgatact ggtcggggcc gaggagagct
     4861 acgaggcccg gtgccgccac tgccacgagg tgccgcggac tatattccag catgggctgc
     4921 cgggggtgta ggtgccccgc cggtgggtta tagctttgct cctcttctgc atcgtcctga
     4981 atgcggccgg ctttatgtgg aagctcttcg agcccgtacc gctctacgac gaggtcgcgc
     5041 acttcctgac gcccttcacc ctcgtggcga ttacggcgga gataatctac aggagcgggg
     5101 gcgacgacga gttcttcgat accccgcggc gcgcggtcct caccggcctg gttatagggt
     5161 tcgcgggggc agtgggatgg gaaggggtgg aagccttgct ggctgcgatg gggttcagca
     5221 tctcgaacgc cctccccgac accatcttcg acgtcttcct cggcgtcgcc ggtggcgcgg
     5281 cgggcgcgtg ggcggcggac cgctacctgg accggatctt caaacgttcg cgcactaacg
     5341 cccgccgtcc ccgcgtccgc tagccagctc tccgggccgg cggcagcgcc tcgcgggcgg
     5401 ccaggacgcg ccgcgcggcc tctcggggat caccggcgag ggacgtcagg tggcccatct
     5461 tgcgccccgg acgcgcctcc gccttgccgt agaggtggag cctgacctcc ggcatagcca
     5521 gggcttttgc ccagtccggc tctcccccgc gccacaggtc gccgagcagg ttcgccatcg
     5581 ccgcgggccg gacttgatgg gtactgccga gcgggagccc gcagacggcg cgcaactgct
     5641 gctcgaactg cgaggtcacc gctccctcta tggtccagtg cccggaattg tgcgggcgcg
     5701 gggcgatctc gttgacgagc agcttcccgt tagcggccac gaagaactcc acgcacgccg
     5761 tcccgacaac gtcgagcgcc tcgaagacgc cgcgcgagac ctcgacggcc tccttcgagg
     5821 tctccggcgg gaggtcggcg ggggccacgg tcaggtccag tatgtggtcg cggtgggtgt
     5881 tctccaccgc gccgtagtgg gcgaaggaac catccaggcc gcgcgccccg acgacggaga
     5941 cctcaccctg gaggtccacc cattcctcca gcaccgcttc gttctccccg agcgtttccc
     6001 aggccgcggc agcctcgttg ggagcttcga tcttcacctg tcccttgccg tcgtagccga
     6061 acccggcagc cttcagaacg gccggcgttc cgatctcctc cagcgcggtc cgcaactctg
     6121 aagccgatcc taccgtccgg tacggtgtta cggggaaccc cgcctctgat aggaaagtct
     6181 tctcgcgcag ccggtgttgc gccgtgtgga gggcgcggat gccgggacga accggggcca
     6241 gcgcctcgat ctcctccacc accgaagccg gtatgttctc gaactccagg gtcaccacct
     6301 cgacgctccc ggcgaactct cgcgcggcgt ccaggtcttc gtaaggggcg gaccactcgc
     6361 ggtccgcccc ctgaccggcg gggctgtcag tatcgggcga gtagacgtgg acccggtagc
     6421 ccatgcgccg ggcggccagg gcgaacatgc gccctaactg accgctgccc agcacgccca
     6481 ccgttgagcc cggcaacact gtctcgctca aggaagctcc gcctccagga cttttctcgt
     6541 ctgctcctga cggaaaacgt gcagcctctc gcgcaactcc ggccgcgagt tagccaggat
     6601 cgccacggcg aggagcgcgg cgttagtggc gcccgccttg ccgatggcga gggtgccgac
     6661 ggggattccg gccggcatct gcgcgatgga gagcagcgag tcaacgcctt ttagcgtccg
     6721 gctctcgacc ggcacgccga gcaccggcaa cgccgtctgg gcggcgacca tccccgggag
     6781 atgggccgcg ccgcccgctc ccgcgatgat gacttcgatg ccacgcgcct ccgcctcctc
     6841 ggcgtagcgg gacatcaggc gcggcgtgcg gtgcgcgctg accacgcgcc gctcgtgcgg
     6901 cacgccgaag tcccgcagcg cctcgaccgc atgacgcatc gtctcccagt cggacgtgct
     6961 gcccatgatg acgccgacca gtgcctcgtt gctctccagg ccagcctccc cggatgagcg
     7021 ggctgatgat tctctcctgt ccatcgtcct ccccggttcg cggcgtgagg gatacagcgg
     7081 gcggcctccg ccattcggtc ttccgccccg aacgacacgg ccgaaacggc ttcggttgca
     7141 aaggcccagc cgccagcgcg gattatacag tttggcatcg tccgtctcat tttgggagag
     7201 attgcgaaga acctgacgct ttccttagat tctcgccgga cgagagttcg tggagcagcg
     7261 tgtgaggatt cggtcaggaa gatgcgagtg tttcgctgga gtatgcttct atccgcatac
     7321 tatcggcggc agggtgaata gggctgacgc cggtgctttc gtcactcgtg cgaacggtgc
     7381 ggctgctccg gccaggttcc aggactgcct aagaaaccgc cgggttcccc aggaggcggc
     7441 cacgttccag gactctctcc aggatccggg cgatgaagcg gactttgctc atgcggacga
     7501 gacctatcag ccgggcggag agcatctccg gggcagggtc cagggcaacc ttgttcgcgt
     7561 ccgcgcagtg gcagtaaagc accgagatga gatctccggg cgaggtccgg cgcaccatcc
     7621 atccgccttc gtcgagtcca aggagatacc ggaacatctc ggcctctccc tctccactga
     7681 agatcgccgg ggtcccgtcg ccgtcgaccg cgagcacccg cccgtccttc tcggcgacga
     7741 tcagccagaa atcggtgcgg gggtacggcg tccttgcgct cgccattcgt atctccttca
     7801 tagccgactc gtataccggg gcagggccgc ccctcggaaa gcggttccgc tttgcgttta
     7861 tattctctcg cgccggtgcg ctgcgcgcca tgtgtcgaaa gatgcaacag cggctgcata
     7921 catggagaca tttatgcttg cggccgttga caggtatgga cgcgtaggat aactattaac
     7981 gcaaccggag cccgtaccct cctttctctt tccccactta tcacccctat ttatacgggc
     8041 ccggttgcta cgtcgcccgg caaattgccc tctcgatgcc cgtcctgcac ggtcttcccg
     8101 cctggcgccg gggcaaggat ggacgggtcg gttcgtcacg atgtgttaac acccgaaaag
     8161 gacggagttt acggtatggc aacaagagcg gtcatcatcg gcaatcccaa ttccgggagc
     8221 gcgggggacg agggatacct cgagcgcttc gccgaaaccc tgcgcgccgg cggcctggag
     8281 gtcgaggtcc tcaacaccga gcgtccggac cacgcaacgg agcttgccgc cgcggccggc
     8341 gaccggctcg tcgtcgccgc cgggggtgat gggacggtga acgaggtcgt gaacggcctc
     8401 tcggaaggcg ccaccctggg catcctgccg ctcggcacgg cgaacgtgct ggcacgggag
     8461 ctcgggctgc cgctggagcc ggaggacgct tgcaagcgga tcctgaccgg caccggcgcg
     8521 cggatggatg tcggggtggc gaccgacgag gagggaacgg agcggcgctt cacctgcatg
     8581 gccggcatcg gtttcgacgc gaacgtcgtc cgcgaggtga cgccccgctt caaacgctac
     8641 ctcaggtcgc tcgccttccc gctggccgcg ctcaaggtgt atttccgggg cgatcagccg
     8701 gacctgcaga tcgtggacgg gaacacgacc tacgtaacgc agttcgccgt cgtcgccaac
     8761 ggccagtact acggaggaga gttcgagatg gccgaggacg cctccctggg tagcggcgcg
     8821 ctggaggtcg tcctcatcga gaaggtgggc ctcctcctga ggcccgacgt cctggcccgc
     8881 atcctggcca agagcccgct ggaccgcgcg atgaagtcct tctcgtcgcg gggtatcctc
     8941 gccaaatccc cggccgctcg cggcgcgcgc gtcccggtgc agatagacgg cgaggtctgg
     9001 gggcgtctcc cgatgtcgtt ccgcatcgaa ccggcggcca tagaggtgat caggtagccg
     9061 cggacggccg cggagccggg cctgctctcc cccagcggta gggcttattc tacgtgcgtt
     9121 cgaccggctg tcataccgcc gttcctttgc ggagcggagc gccgacttcg tgcaggtgtt
     9181 gcaggatctc ccggtaggac tgcagcacgc tggtctcgtg ataggcgatt gagtgaacac
     9241 gacagaagtc cttgatgatc ggctgcgctt cccgcagctt gttgcggggc atcctcggga
     9301 agaggtggtg ttcgatctgg tagtttaggc cgccgtacca gaagtcggtg accgggtgcg
     9361 cgaagacgtt ccggctggtc agcacctgac ggcgcaggaa gtcctcctcg ctgtcctgtt
     9421 ccagtagcgg catggctttg tggttcggcg cgaagatcga gaccatgtac aacccgtaga
     9481 gaccgcgatg aacgatgatg aacagcaccg cctgcaaggg ctccagcgta gagaacagca
     9541 agacgaagta caagacgaag tgcgctccca tggcgagcac ctcggccaga gcattattcg
     9601 ccttcctcgc catgaggaac gtgatgctgc tgcggagcat actcagggcc tgcaggagcg
     9661 atagcggaaa gatcaagacg gcctgatact tcacgacgaa ccgcgcgatc ccccgcttgt
     9721 ccagggcctg ctcttcttcg aaggccagta gcggtatgtc gatgtcggga tcgacgtcga
     9781 tctggttcgg gtgtccgtgg tgctcgttgt gcttgtcgat ccaccactgg cggctcatac
     9841 cgagtaggaa gttccccaga acgagggtga gcaagtcgtt cttccacggg gcgcggaagg
     9901 agatttgccg gtgcccggag tcgtgggcga gaaggctgat ctggacgaag acgaaggcca
     9961 ggtaggcggc gtttagaagc tggatccagg tgttgcccga gatcagcagt aaggtcaggc
    10021 ttaccgccag caagccgaag gtaagtatgc tcttgcccgc gaagtaagcg ggctgctgat
    10081 ccagtagccc ttcctgcttg atgagacgct tcaactcggc atattggttg gtccgagagc
    10141 gggtcgcgga tctcctggtc tcgcccggcg cctcgtccgg gtatggccta tccaaattca
    10201 cgaagtacgg tcctttctaa cctggtgttg ctccgggccg gctggttcgc tcgcgctttg
    10261 gacatcaggc cttcgacgtt ctcacttatc ttgagaaccg acgagaagat atgtagtcca
    10321 tacacgcttt agaagacggg taacgagaat atagccaccc tggaccatct caaactctgt
    10381 ttcggctgtt tgacggcgta aatgtgtcga agtgtgcacc gaacaagcct cacaactagc
    10441 cggtggggcg tccgccgagc gtgtgctcag agtacgcggc catcgagagc ttgctcctgt
    10501 gagcttcgtt gccggaatca gagcatttgg atagaatgat gactttccta cgatcagact
    10561 ctcccggcca ggagggccca actctgaagg cggggagtgt ggacgaaaga agcaaaatcg
    10621 gcgcgatcag ggtgcttttg gctgacgacc acaccatgtt ccgccagggt atcgcggggg
    10681 ttctggaaga gtacggcggc atggaggtcg tcggccagac caacaacgac gagagggccg
    10741 tggaactggc ccgacggacc aggcccgacg tcgtcctgat gcaggcgcag atgccgatcc
    10801 agagggccag ggagaacctc cggcggatgc gcgagaacgt cgagcccccg ccgaaggtcg
    10861 tcatagtcac gatgatcgag gagccgcgcc gcgtccggga gataatgaac ctcggggtga
    10921 gcgcctacat cgtcaagagc gcctcggccg agcacctggt ggcggcggtt cgggccgcga
    10981 tcctggaccc gaagagcaag gacgtcgtgg tgggcatgcc gctagatctg ctggaagaag
    11041 ccgagaaagg ctcgcggggc gtgctctctg tcagggagct tgagatactg ctcctggctg
    11101 ccaggggtct atccaaccgc cagatagcga actccctgca cgtggcggag gggaccgtca
    11161 agcgccacct cgccaacacc ttcgagaaga tgaacgtgcg ttccaggggc gaggccacca
    11221 ggatggctct tcacgaggag tggatcacca tccaggacgt caccgaagac ctcgaagagc
    11281 ccgaagagag cggctagacc tccggggagg gttccgacgg gccgccatcg gcggagatta
    11341 gcttctctgt ctcgtccagg gccgcgagga attcctccag gctcacgtca tggatctcgg
    11401 agatccttcc gtccacgctg atgcgcgcgt tgaggtggtt gtcgatgatg ataccgaccc
    11461 agcagacgac gccgtccggc gctcccgctt cgaccccgtc atcgtcccgc acgaggacgc
    11521 gcggcgtcag acgttccacc tgcccatcga gagtcttggg gtcggagatc agcacctcgt
    11581 aatgggggcg gcgctgttgg atggagagag ccagggactc ccggtacatc cgtggcgtgt
    11641 tcgcgatcaa tattcgcaaa ttgttcgtgt tcctctctgt gcagacaaag cccacgagag
    11701 agaacgcacg cagttatcgc ctacccggta gggtgtatgt gtgaatctta caccctgcgc
    11761 gggcgtaccc tgtgcgtatt gcgggcgttt gcgcccttcc cgcgtaaagt agtactaaat
    11821 gcccaggcta atatgtgtca aaaggtgcaa tactacgtgg cagccaactc gtacaataag
    11881 taactgcgat accaagaaca cgagtgcctt tcttcaacct gagtctccac gggctcccgc
    11941 tctgttaacc ggtgaggtaa tcggactcca gccaagggag ggccagcgtg cctcgggaag
    12001 cctataacca ggacaaggaa cagcggaata ccgaccggcg gactcgctgc gaatttggtg
    12061 cgacgcttat gggaacagcg tgtgaggtac cgggcgaggt ccggtacgcc ggggggtgct
    12121 tgtgcgtgaa ccatgccctg ctgatcgcgc tggatgagcg cgccaacacc ttgatggacg
    12181 tggtgtttac gctggacagg ttggcggagg ctcccgacct gcgcgcggac gagctgcggt
    12241 tgcgccgcct gaagcatcag atagaagaag ctgagtacca gctaaggatc agccgcatgg
    12301 agctggatct cgcccgcatg cggatgtagg tgtcaatacg agcgagccgg gaccctgagc
    12361 ttgcggttgg cgagcacaac cgcaagctct cctacaggcg ctcctcgcta tgattgagcc
    12421 acccgtcctg gttctgcttg agccacgtag tccgcaacct gttggacgcg atcctggccg
    12481 ggcgatcttc cggagtgaga tcgtcgaggc gcttgtagga caccttcccg ccgctcgtga
    12541 gcgccaccag tccttcttcg gcctgtcttt ggtcctcgga gaccagtcgc aacaccgccg
    12601 gcaccgcagg ggtggttccc cgcatcctgt ccagcaccct ggcgagttgg ccgtccttcc
    12661 gcagctccag gtcccgcatc tccgattgca tgaacaactg ccacgaggcc tcttgtagta
    12721 ctcggctatg ttcctctctt cgggttcgac gacgctcctt ggccctctta ggcgcaaggt
    12781 tttcctgcca ctgctccgcc ctctcatcgc agtgcggacc gtcgcgcagt ttcccaagct
    12841 gtcttccggc ctcggtctga agaactgcgt gcatcgcgac accgaacggc tcaccggtgc
    12901 gctcggatag gaccatcgcc tgccgagata gcaccccgct aaccatgtcg gacactagtt
    12961 gcttcttgtg catggctttc acctttcccc cgagccgcct cgccgctgcg aggatttcct
    13021 ctaaaaaggc cgacttgctc aacacctcta ccgctcccaa ctcccgtaac cgggcgtgga
    13081 tctccgggtt ctcggttgcg gtcagcacca tcaccgggat acggcgcgta acctctcgca
    13141 gcgccccgat ctccgcgatc aggtccgttc cgtctccatc gggcaacgca aggtcgatga
    13201 tcgcggcatc gatctcctcc gcttcgtcgg ctgacaagct gtcggctgac aagcaagcgc
    13261 gggcctcggc cagagatccc gcctgggtgc tcaccctgaa gtccgtctcc caacccaaca
    13321 ccataccgaa agcctgacgg aaccaggcgt ggtcatctac cagcaggatg cggctcatat
    13381 tcccacatgc tctcgtcgga cccggtaccc cgccccgacg agtggctcgg agccgaggtc
    13441 ctgcgcttcc tgggtaagct ctcctatggc gaagtacgcc tcctgctcgc gggcgcacgc
    13501 tttgcagaag ggtatgccat agatctttac caccgctcga ttgtggcagg ggtgctccgt
    13561 cttgcttcgc acctggcacg gtcctagtcg cttcgtcgtt gcttcggtca agagaatctc
    13621 cttctgtcat gagccgaacc ggcgttcgct cctgagagta atcttaggtg gcaaaaatat
    13681 tcgggctggc gaagccgctc ctgtgaggag tcggcgatcg ccggcccgtg ctacaaacgc
    13741 cggtcgtgcc acaacgccgg tatctgcggc tggttagtcg accttcctgt acatcattcc
    13801 cacctcccac tagaggtaca atgacttgcg ctagtattgt accacttatt actaaaactg
    13861 taccgcgctc atggcccgcc ccactgctat cgctatccat ccgaggtacc ttgggttatg
    13921 cacggccagc gcaatcacaa ggccgaagag gagcaggacg atccccccac tgagcatccc
    13981 gtccacggcc tggttgatag cgtagatgcc ctcggcaacc accgcgacaa cgatccccat
    14041 gcgtgccagg gcgacgctcg ccccctgcgt gatggagcgg tacagggcta cgaagccgac
    14101 gagcaggagg acctctccag cgaacttact cggctgcggt ttggcggagc tgggggcttc
    14161 ccttgtcagg caagggttcc gaggcttgct ctcgccgttc agaccacagc gcatagccca
    14221 ggcaggccag agccaacccc atcggcatag ccgctaaccg ctggatctca tgcgggagca
    14281 gcgcagccag gggggtcaac gcggcagtga cggcgagtag accggccgcc cagcgcggca
    14341 ggataccagc gcggaacgta gcgatgccga acagcaggcc gccgagcata tagaggatcc
    14401 ctgctagagc aaatagtgct gggatggctc cgaggccaac ctcgctggcg cggctactgg
    14461 cgacccccag gaagccatcc acaaacttcg gcgctacgct cgccaacggc ggcatgataa
    14521 aggtttcggc gaagacaaag gctgtaataa gcgcccaacc aaggccaaat aggagaaagc
    14581 ctgccaggcc aagccaaccg gcctctctca cttgccgggc atagatcccc gcaatgccca
    14641 gcaagaggag gaggcacata accgtcttca aaggggtgat aattgcccat gcgccggtgg
    14701 tgactgacga aacaacgtca ggcggatgga tcggctgaat gcccgcgaaa atgatccctg
    14761 cccccatagc cgataaacct gcccaacgga tgagattcga agctgttatc ttcattttgc
    14821 tctcctttcc gttgactgcc atttttcgtt ctcctctctc aacttactgt ttgtcgtcgg
    14881 acgcatccgt ctaggggggt ttgacgcgaa cggccgagat cagtggcatt cccagatcac
    14941 gc
//
LOCUS       DASSMB010000143         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1553761, whole genome shotgun sequence.
ACCESSION   DASSMB010000143 DASSMB010000000
VERSION     DASSMB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1553761"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /gene="pcaH"
                     /locus_tag="VFJ72_08610"
     CDS             complement(<1..593)
                     /gene="pcaH"
                     /locus_tag="VFJ72_08610"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET7479558.1"
                     /translation="MAQQARERGAGSDSGSDSAGSHPPYLYPDYVATRLRAPKKPLII
                     VPRTLSDTTGPAYGRDPIGELDSDLTRQHSGEPLGERIIVTGRVLDGDGRPVRNSLIE
                     VWQANAAGRYIHKGDRHPAPLDPNFSGAGRCLTDNDGNYRFVTIKPGAYPWKNHDNAW
                     RPAHIHFSLFGPAFRTRLITQMYFPGDPLFGQDPIFQS"
     gene            711..1634
                     /locus_tag="VFJ72_08615"
     CDS             711..1634
                     /locus_tag="VFJ72_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7479559.1"
                     /translation="MVYGSAMDLRRLRYFVVVAEEQNFSRAAERLHMAQPPLSDQIKR
                     LEKSLGVKLFDRSSRGARLTEAGELLLTEARRLLVQADQTAEMVRRVGGGEVGRLALG
                     FVPSASNSVLPPVLSTFRERYPDVQLYLQEMNPDWLVAGLHEGRLDVSFFYLPFEDRA
                     LAHEPVSREPLVVALPETHELAAEDKIDLCDLADQPFVLPARYRMPGLHGQVLEACRD
                     AGFEPEAVQKEVWLMQTIVALVAGGIGVALVPASVRNTPRAGVVYKEVLGLSPTVEMS
                     VVWRRNERNPVVRSFLKVVDEICCGNSWSAE"
     gene            2007..>2492
                     /locus_tag="VFJ72_08620"
     CDS             2007..>2492
                     /locus_tag="VFJ72_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479560.1"
                     /translation="MSQTAEPRNSDYPVDQDFDPLDPDYLADPYPYYARFRREAPVFY
                     APKIDFWVVSRYEDILNIVKDPETYSNRRVQEPLYALTPEALGKLKEGVRVTPTTSTA
                     DPPLHRRTRKHAARAFSARRVAGLEGRIREVANGLIDRMISDGRADMVHQFAFPLPAS
                     VV"
BASE COUNT          427 a          806 c          829 g          430 t
ORIGIN      
        1 gactggaaga tcgggtcctg cccgaagaga gggtctccgg ggaagtacat ctgggtgatg
       61 agtcgtgtcc tgaacgccgg gccgaagagc gagaagtgta tgtgcgccgg ccgccaggcg
      121 ttgtcgtggt tcttccacgg gtacgcgccc ggcttgatcg tgacgaagcg gtaattacca
      181 tcgttgtcgg tgaggcacct acccgcgccg gagaagttcg ggtcgagcgg cgccggatgc
      241 cggtcgccct tgtgaatgta tcgcccggcg gcgtttgcct gccagacctc gatgaggctg
      301 ttcctcaccg ggcgcccgtc accgtcgagc acgcgccccg tgacgatgat cctctctccc
      361 agcggctccc ccgagtgctg gcgcgtcagg tcgctgtcaa gctcccctat cgggtcgcgc
      421 ccgtaggccg ggcccgtggt gtccgagagg gtcctcggga cgatgatcag gggcttcttc
      481 ggcgctctca ggcgcgtcgc cacgtagtcg ggatacagat acggtggatg actgccggcg
      541 ctatcggaac cgctatcgga cccggcgccc ctctccctcg cctgctgtgc cattgtctct
      601 ctcctttcct cgcccccaat tatgcaactg cggtaattct gtcgcctccc ttctgatatg
      661 tccaatacca gtttgacgac cttgatatat gttttttgta taatgcgtgt atggtctatg
      721 gttcggcgat ggatctccgg cggttacgct acttcgtggt ggttgcggag gagcagaact
      781 tcagccgggc ggcggagagg ctgcacatgg cgcagccgcc gctgtcggat cagataaagc
      841 gcctggagaa gtcgctcggg gtgaagctct tcgaccgttc gagccgcggg gcgcgtctta
      901 cggaggcggg cgagttgctc ctgacggaag cgcggcgact cctggtgcag gcggaccaga
      961 cagccgagat ggtgcgccgg gtgggcggcg gagaggtggg acggctcgcc ctggggttcg
     1021 tcccctcggc ctccaacagc gtgctcccgc ccgtcttgag caccttccgg gagcgctatc
     1081 ccgacgtgca gctctacctg caggagatga acccggactg gctggtcgcg gggctgcacg
     1141 agggccggct cgacgtgagc ttcttctacc tgcccttcga agaccgggcg ctcgcccacg
     1201 agcctgtctc ccgcgagccg ctggtcgtcg ccctgccaga gacgcacgag ctggcggcgg
     1261 aggacaagat agacctgtgc gacctcgcgg accagccgtt cgtgctgccc gcccgctaca
     1321 ggatgccggg cctccacggg caggtcctgg aggcgtgccg cgacgccggc ttcgagcccg
     1381 aggccgtgca gaaagaagtc tggctcatgc agaccatcgt ggcgctggtg gcgggcggaa
     1441 tcggggtggc gctcgtcccc gcctcggtgc ggaacacgcc cagggccggc gtcgtctaca
     1501 aggaggtgct cggcctctcc cccacggtcg agatgagcgt cgtctggcgg cgcaacgagc
     1561 gtaaccccgt cgtgcgctcc ttcctaaagg tggtcgacga gatctgctgc ggcaacagtt
     1621 ggagcgcgga gtagcgcgac tccccggaga gcgtggttca ccgcagaaac cgtaacggcc
     1681 ccaacgaccg gattacgtac ctctgccata ctcaaaacat gcatcggcgg tacgttttcg
     1741 gtatttgaac cgggctccgg cgtccctcaa aatgaggggt aaggggaaag cgagagccag
     1801 ggaaagaccc taacgcatga aagaaagacc gaaccttgcg cgggctccag acttcgagag
     1861 tctgcgggca aagcgttgag tccgacgcgc atacaggcat cgggcgagga gatcggagcc
     1921 ggcgcaggtc cctcgcgtcc acggcacacc cacgccggac ataaggcatg tagcagccgc
     1981 cttcgacctc acccgagagg agcacgatga gccagaccgc cgagccacgc aactcagact
     2041 atccggtgga ccaggacttc gacccgctgg acccggacta tctcgccgac ccatacccgt
     2101 actacgcccg gttccgaagg gaagcgccgg tcttctacgc gccgaagatc gacttctggg
     2161 tcgtcagccg ctacgaggac atcctgaaca tcgtcaagga ccccgagacc tactcgaaca
     2221 ggcgcgtgca ggagccgctc tacgcgctga ccccggaggc gctcgggaag ctgaaagaag
     2281 gcgtgcgcgt aacgccgacg acctccaccg ccgacccgcc gctgcaccgg cgcacgcgca
     2341 agcacgccgc cagggcgttc tcggcgcgtc gcgtcgccgg gctggaggga cgcatccggg
     2401 aggtggctaa cggcctgata gaccgcatga tctccgacgg tcgcgccgac atggtgcatc
     2461 agttcgcgtt cccgctgccg gcgagcgtgg tc
//
LOCUS       DASSMB010000144         3260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1426760, whole genome shotgun sequence.
ACCESSION   DASSMB010000144 DASSMB010000000
VERSION     DASSMB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426760"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFJ72_08625"
     CDS             <1..582
                     /locus_tag="VFJ72_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7479561.1"
                     /translation="ETVVHRHVETFPLDEKGQGDADVQYAFSAHRAVVDVDTELGLVR
                     VVELATAQDVGKALNPQSVEGQIEGGTAQGLGLALLEEIQVKDGKILNASFTDYLLPT
                     ILDMPPVKMDVLELADPGAPYGLRGVGEPPNISTPPAVVAALRDATGRELPRIPVRPE
                     QIAGISGGSPRRREQAARGGYADIGALDERSET"
     gene            582..890
                     /locus_tag="VFJ72_08630"
     CDS             582..890
                     /locus_tag="VFJ72_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479562.1"
                     /translation="MPGYNRAELNVEFKIVPSSRAEKSPREQVEAGIRVADDSGLAAH
                     ESGPDTREISGGRAEVLETVMKVIEASLDAGARIVELKVEAQEEAGKFGEPGGRRDMR
                     "
     gene            937..2931
                     /gene="uraD"
                     /locus_tag="VFJ72_08635"
     CDS             937..2931
                     /gene="uraD"
                     /locus_tag="VFJ72_08635"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HET7479563.1"
                     /translation="MSQTTTAERPQIQRTHPVDRVLPPGKLAVYGFQHVLAFYAGAVI
                     VPILLAGAIGLSGQQLAFLINADLFTCGIASIIQAVGFWKVGVRLPLLQGVTFTAVAP
                     MIAIGNANGGGVKGLLAIYGAVIVAGILTFLIAPYFSRLLRLFPPVVTGTVITVIGFT
                     LVPVAAMDAGGGNPQAQDFGSLQNLAFAGGTLLFILLIYRFFRGFLSTIAVLLGLVVG
                     SAVAWAFGLVDFGAVGQSSWVGVTTPFYFGLPTFGVAAIVSMVVVMLITAVETTGDVF
                     ATGEIVEKPIRREDIARAIRADGLATTLGGLLNAFPYTCFAENVGLVRLTRIKSRWVV
                     AAAGAIMIIFGLLPKMGAIVAAVPRPVLGGAAIVLFATVGVIGIQTLSRVDFHDERNV
                     IIVAVSVGLALIPVAFPTFYQNFPPQLQIVVGSGITTGSLAAILLNLIFNVLGGRSNL
                     VDEVDPTPRHAERITLDQVNHLSREEFVERFGTLFQGPDWVAEAAYEQRPFESVYDLR
                     RAFQDAMFEAPAERQLGLIRSYPDLGSMVGAEQEATSVGIPREDYEMLKSASVGRILG
                     PESLRDQSSAGLDRLTPEEYERFHRLNTAYREKFGFPMVFCVREHTKETILANGEARL
                     ENPPAQERATALVEIAKIANLRLDDLVEHQPREEEAVANV"
     gene            3054..>3260
                     /locus_tag="VFJ72_08640"
     CDS             3054..>3260
                     /locus_tag="VFJ72_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7479564.1"
                     /translation="MIFPSVVLSLPGWVEEYLPDPEREYPTEEDRMRLVIELSRLNVE
                     HGTGGPFGAAIFDLETNRLVAPGIN"
BASE COUNT          547 a         1043 c         1149 g          521 t
ORIGIN      
        1 gagacggtgg tgcaccggca cgtggagacc tttccgctgg acgagaaagg ccagggggac
       61 gcggacgtgc agtacgcctt ctcggcgcac cgggcggtcg tggacgtgga cacggagctc
      121 gggctggtac gggtggtgga gttggcgacg gctcaggacg tggggaaggc gctaaacccg
      181 caatcggtgg agggtcagat cgagggcggg acggcccagg gcctggggct ggcgctgctt
      241 gaggagatcc aggtaaagga cggaaagatc ctgaacgcct cctttaccga ctacctgctg
      301 ccgaccatcc tggacatgcc cccggtgaag atggacgtgc tcgaactggc cgaccccggc
      361 gcgccctacg gtttgcgcgg tgtgggcgag ccgccgaaca tctccacgcc gcccgcggtc
      421 gtcgccgccc tgcgcgacgc aacgggccgg gagttgcccc gcatccccgt gcgccccgag
      481 cagatcgccg ggatctccgg cgggtcgccg cgccgccgcg agcaggccgc gcggggcgga
      541 tacgctgaca tcggcgccct ggacgagagg agtgagacgt aatgcccgga tacaaccgag
      601 cggagttgaa cgtcgagttc aagatagtgc cgtcctcgcg ggcagagaag agcccgcggg
      661 agcaggtcga ggcggggata cgggtcgccg acgactctgg cctcgcggcg cacgagtcgg
      721 gaccggacac cagggagatc tccggcgggc gcgcggaggt tctggagacg gtgatgaagg
      781 tgatcgaagc gtccctcgac gcgggggcgc gcattgtgga gctgaaggtg gaggcccagg
      841 aagaggccgg gaagttcggg gaacccggcg gaagaaggga tatgcgctga ccgcgcagtc
      901 tcccaaaacc aaagcaactg gtaaggaggt aagaacatgt cgcagacaac gacggccgaa
      961 aggccacaaa ttcagcgtac tcatccggtg gacagggttc tcccgccggg caagctggcg
     1021 gtctacgggt tccagcacgt actcgcgttc tacgcggggg ccgtgatcgt gccgatactg
     1081 ctcgctggtg ctatcgggct ctcgggccag cagctcgcgt tcctcataaa cgcggacctg
     1141 ttcacctgcg ggatcgcctc gatcatccag gccgtcggtt tctggaaggt cggggtgcgg
     1201 ctcccgctcc tgcagggcgt aacgttcaca gcggtcgcgc ccatgatcgc catcggcaac
     1261 gccaacggcg gcggggtaaa gggcctgctc gccatctacg gggccgtgat cgtggcgggg
     1321 atactcacct tcctgatcgc gccgtacttc tcccgcctcc tgcgcctctt cccgccggtc
     1381 gtcaccggca ccgtaataac ggtcatcggc tttaccctgg tgccggtcgc ggcgatggac
     1441 gcgggcggcg gcaacccgca ggctcaggac ttcggcagcc tccagaacct ggcgttcgcc
     1501 ggcggcacgc tgctgttcat actcctgatc taccgctttt tccgcggctt cctcagcacc
     1561 atcgccgtgc tcctggggct cgtcgtcggc agcgcggtgg cgtgggcgtt cgggctcgtg
     1621 gacttcggcg cggttggtca atctagctgg gtcggggtga cgacgccgtt ctacttcgga
     1681 ttgcctacct tcggggtggc ggcgatagtc tcgatggtcg tcgtcatgct gatcacggcg
     1741 gtcgagacca cgggcgacgt gttcgccacg ggcgagatcg tggagaagcc catccggcgc
     1801 gaggacatag cccgcgcgat ccgggcggac gggctggcga cgacgctcgg cgggctgctg
     1861 aacgctttcc cgtacacctg cttcgcggag aacgtggggc tcgtgcggct cacccggata
     1921 aagagtcgct gggtcgtggc ggcggcgggt gcgatcatga tcatcttcgg tctcctgccg
     1981 aagatggggg caatcgtcgc ggccgtaccc cggccggtgc tcggcggcgc cgccatcgtc
     2041 ctgttcgcca cggtcggcgt gatcggcatc cagaccctct cgcgggtgga cttccacgac
     2101 gagcggaacg tcatcatcgt cgcggtcagc gtggggctgg cgctcatccc ggtggcgttc
     2161 ccgactttct accagaactt cccgccgcag cttcagatcg tggtgggaag cggcatcacc
     2221 acgggcagcc tcgcggccat cctgctgaac ctcatcttca acgtgctggg cgggaggagc
     2281 aacctggtgg acgaggtgga cccgacgccc cggcacgctg agaggattac gctggatcag
     2341 gtcaaccacc ttagccggga ggagttcgtg gagaggttcg gcacgctgtt ccagggaccg
     2401 gactgggtcg ccgaggcggc ctacgagcag cgtcccttcg agagcgtcta cgacctgcgc
     2461 cgcgccttcc aggacgcgat gttcgaggct ccggcggagc gccagctcgg cctgatccgg
     2521 tcctaccccg acctgggcag catggtcggc gccgagcagg aggcgacgag cgtcgggatc
     2581 ccgcgcgaag actacgagat gctgaagtcc gccagcgtcg gacggatcct cggccccgag
     2641 tcgctgcgcg accagtcctc cgccgggctc gaccgcctca ccccggagga gtacgagcgg
     2701 ttccataggc tcaacacggc ctaccgggag aagttcggct tcccgatggt cttctgcgtc
     2761 cgggagcaca cgaaggagac catcctcgcc aacggcgagg cgcggctgga gaaccctccg
     2821 gcccaggagc gggcgaccgc cctggtcgag atcgccaaga tcgccaacct ccgcctggac
     2881 gacctcgtcg agcaccaacc ccgcgaggag gaagccgtgg caaacgtctg agatcctcga
     2941 accgggaagc gagcaggaac acctggttcg cctcccagtg ccgcagagac cgtgacaaat
     3001 ccgccgaagt agctacagcg taaggcgccg gtgcgcgccg gagaggtgat gcaatgattt
     3061 tccctagcgt ggtcctgagc ctgcccggat gggtggagga gtacctcccc gatccggagc
     3121 gcgagtaccc gaccgaagag gaccggatgc ggctcgtgat cgagctgtcg cgcctgaacg
     3181 tggagcacgg gaccggcgga cctttcgggg ccgcgatctt cgaccttgag acgaaccgcc
     3241 tggtcgcccc cggcattaac
//
LOCUS       DASSMB010000145         4707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1679210, whole genome shotgun sequence.
ACCESSION   DASSMB010000145 DASSMB010000000
VERSION     DASSMB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1679210"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1497
                     /locus_tag="VFJ72_08645"
     CDS             <1..1497
                     /locus_tag="VFJ72_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7479565.1"
                     /translation="DSIPQICITGQAPTNVLHKEAFQAVDIVEIAKPVTKWAVQVREP
                     AQLVWTFREAFRIAQEGRPGPVLIDLPLDVQTSGLEVDFDAERGGRLHFEKPEPRPNA
                     IRQVVEMILEAERPVLMPGGGVIISDASEDLVELAEYLQVPVSPTYMGKGAINEDHPL
                     YMGIVGLQTQQRYANALFLESDLVVGIGNRWAERHTGDLDVYRGDRKFVHIDIDPRQI
                     GRVFPPDLAVVSDAKLALEAMLQTARDMTSERESDEWVDRVDELRSTLLRKTDFDDSP
                     VRPQRAYQEMNEFFDEDAIMVTAIGLYQIFGGQFQKTYKPRHYLCCGQAGPLGWEVPA
                     CIGVKLGRPDNLVVGVVGDYSFQFLMEEIAVAVQYKIPYVIVMLNNGYMGLIRQSEMK
                     YDMNFAVDISYDGPDSDYGIDNVMVMEAMGAFGRRVSNPEEIQDVLAWAVETSEERRV
                     PVLVEIMCEREVNAAMGVSIDKINEYEPILDGTREKAGQVVGGIPERD"
     gene            1502..2794
                     /locus_tag="VFJ72_08650"
     CDS             1502..2794
                     /locus_tag="VFJ72_08650"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET7479566.1"
                     /translation="MKNELREILSAALEAADPGEAVRRYLNVEDGKILAADASFEPRR
                     VFVLAAGKAACAMARAAEELLGDRVSGGLVVTKDGNETPLERLEVVLSSHPEPDERGV
                     EAARRVAELAESLEEGDLLLALISGGASALLADPAPPIELADLKKLTGDLLRSGASID
                     EINAVRKHVSMLKGGGLVRSAAPADTLALLLSDVVGDDLSSIASGLTAPDPTTLQDVE
                     RIVRRYDLKLPESVRDHLDGAPETPEPGDPVFERVTNLLVGGGRLTSEAAAEKASDLG
                     YEPLILSTYVTGQARNVAGLYAAIVREVRDSGNPLQPPCAIISGGEATVVVRGDGVGG
                     PNQEFALALAVELEGVESWAAFAVDTDGNDGPTDAAGGMVDGETAKTIRESGLDPLEA
                     LDDNDTYKALSAAGALIQTGPTGTNVNDLRVVLLAAPK"
     gene            2836..3810
                     /locus_tag="VFJ72_08655"
     CDS             2836..3810
                     /locus_tag="VFJ72_08655"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET7479567.1"
                     /translation="MTIMPSRERFRGCLVGQALGDALGFVVEGRPPEVCARYLDEGPG
                     GHDFGQYSDDTQLARELMRSYADLGRFEPADYARRIRDIFSEGRIVGYGRSTAQAAGR
                     LVRDVPWDEAGTPPPSAGNGSAMRAGPIGLFFHDDPEGLVRAATQQGIITHADPRCSA
                     GAIAIAGAVSLVLSDRPVDPAFLLDRLAGLTGEVEPRFAANLRELLRWLSLPPEEAVT
                     RISGTGLDPDQGREWQGIPPFVVSSVLWSLYSFLTSPEDYWRAIRTAIAVGGDVDTTA
                     AMAGAISGAHLGLDSLPGDLTRHLTDRGTWGLADLIRLADECHSVRVR"
     gene            3920..4462
                     /gene="hpt"
                     /locus_tag="VFJ72_08660"
     CDS             3920..4462
                     /gene="hpt"
                     /locus_tag="VFJ72_08660"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET7479568.1"
                     /translation="MKPETGEILVSAEQIAEKVREMGARITEDYRGERPLLVGILRGA
                     VVVLADLMRHIELQCEIDFMEVSSYGTGTSSSGVVRILKDLEEDLTGRHVLIVEDIID
                     TGLTLSYLRRSLLTRKPASLEFFTLLSKPSRRRVDLDVKYVGFEVPDEFVVGYGIDYA
                     GYFRNLSDIYALKPDTAIPE"
BASE COUNT          857 a         1512 c         1553 g          785 t
ORIGIN      
        1 gactcgatac cccagatctg catcacggga caggccccca cgaacgtgct gcacaaggag
       61 gcgttccagg cggttgacat agtcgagatc gccaagcccg tgaccaagtg ggcggtgcag
      121 gtgagggagc cggcgcagct cgtgtggacg ttccgcgagg cgttccgcat cgcccaggag
      181 gggcggccag gtcccgttct tatagacctg ccgctcgacg tgcagacctc tgggctggag
      241 gtggacttcg acgcggagcg cggcggacgg ctgcatttcg agaagcccga gcctcgtccg
      301 aacgccatac gtcaggtggt ggagatgatc ctggaggccg agcgtccggt actgatgccc
      361 ggcggcggcg tcatcatctc cgacgcctcc gaggatctgg tggaactcgc cgagtacctg
      421 caggtcccgg tcagcccgac ctacatgggc aagggcgcga tcaacgagga ccatccgctc
      481 tatatgggca tcgtcggtct ccagacccag cagcgctacg cgaacgccct cttcctggag
      541 agcgacctgg tcgtggggat cggcaaccgc tgggccgagc ggcacacggg cgatctcgac
      601 gtgtaccggg gtgacaggaa attcgtccac atagacatag acccgcggca gatcgggcgc
      661 gtcttccccc cggatctcgc cgtcgtctcc gacgcgaagc tggcgctcga agccatgctc
      721 cagaccgccc gcgatatgac ctccgagcgg gaatccgacg agtgggtgga ccgggtggac
      781 gagttgcgtt cgacgctctt gcgcaagacg gacttcgacg actcgcccgt ccggccgcag
      841 cgggcctacc aggagatgaa cgagttcttc gacgaggacg ccataatggt gacggccatc
      901 ggcctctacc agatcttcgg cggccagttc cagaagacct acaagccgcg ccactacctc
      961 tgctgtggtc aggccggtcc gctcgggtgg gaagtcccgg cgtgcatcgg cgtcaagctc
     1021 gggaggcccg acaaccttgt cgtcggtgtc gtgggcgact actcgttcca gttcctgatg
     1081 gaggagatag cggtcgcggt tcagtataag attccgtacg tgatcgtcat gctcaacaac
     1141 ggctacatgg gcctcatccg ccagagcgag atgaagtacg acatgaactt cgcggtggat
     1201 atctcctacg atgggccgga tagcgactac ggcatagaca acgtgatggt gatggaggcg
     1261 atgggcgctt tcggtcgccg ggtcagcaac ccggaggaga tacaggacgt tctggcctgg
     1321 gccgtggaga ccagcgagga gcgccgggtg ccggtgctgg tcgagatcat gtgcgagcgc
     1381 gaggtcaacg ccgcgatggg cgtctcgata gacaagatca acgagtacga gcccatcctc
     1441 gacggtacgc gtgagaaggc cggacaggtc gtcggaggca tcccggagag ggactagacg
     1501 aatgaagaat gagcttagag agatcctgtc ggcggccctg gaagccgccg accccggaga
     1561 agcggtgcgc cgctacctga acgtcgagga cggaaagata ctcgctgcgg acgcttcgtt
     1621 cgagccccgg cgagtcttcg tgctggccgc gggcaaggcc gcctgcgcga tggcccgagc
     1681 cgccgaggag ctgctcggcg acagggtatc gggcggcctc gtcgtcacca aggacggaaa
     1741 cgagacgccc ctcgaacgcc tggaggtcgt tctctcctca caccccgagc cggacgagcg
     1801 cggcgtcgag gcggctcggc gggtcgcgga gttggcggag tcgctggagg agggagacct
     1861 actgctcgct ctaatcagcg gcggagcatc cgcgctcctg gcggaccccg cgccgccgat
     1921 agagcttgcg gacctcaaga agctcaccgg agacctgctc cgaagcgggg cctcgataga
     1981 cgagatcaac gccgtcagga aacacgtctc tatgctcaag ggcggcggcc tcgtccggag
     2041 cgcagctccc gcggataccc tcgccttact cctctccgac gtcgttggcg acgacctgtc
     2101 gtccatcgcc agcggattga ccgcgcccga cccgacgacg ctccaggatg tagagaggat
     2161 cgtgcgccgc tacgatctga agctgcctga gagcgtgcga gaccacctgg acggcgcccc
     2221 cgagactccg gaaccaggcg accccgtctt cgagcgggtg acgaacctcc tggtcggcgg
     2281 cgggcgcctg acctccgaag cggcggcgga gaaggccagt gacctcggtt acgagccgct
     2341 catcctctcc acctacgtca cgggccaggc gcggaatgtc gccgggctct acgcggccat
     2401 cgtcagggag gtccgggaca gcggcaaccc gctccaaccg ccctgcgcca tcatcagcgg
     2461 cggcgaggcc acggtagtcg tgcgcggcga tggagtcggc ggaccaaacc aggagttcgc
     2521 cctcgctctc gccgtggagt tggaaggcgt agagagctgg gccgcgtttg ctgttgacac
     2581 tgacggcaac gacggtccga cggacgccgc cggaggaatg gtcgatggtg aaacggcgaa
     2641 gaccattcga gagagcggcc tggatcccct ggaagctctc gacgacaacg acacctacaa
     2701 agccctatca gccgcgggcg ccctgatcca gaccggtcct accggcacta acgtgaacga
     2761 ccttagagta gtcctgctcg cggcgccgaa gtaacttgta aggcggtaag ggccgtaccg
     2821 tagaggaagc gcgcgatgac cattatgccc tccagggagc ggttcagagg atgtctcgtg
     2881 ggacaggctc tcggtgacgc cctggggttc gtggtggaag gccgtccacc cgaggtctgc
     2941 gccaggtacc tggatgaggg tcctggtggg catgacttcg gacagtattc cgacgacacc
     3001 cagcttgccc gcgagctcat gcggagctat gcggatcttg gcaggttcga acccgcggac
     3061 tacgcgcgca ggatacgtga catcttctcc gagggccgga tcgtcgggta tggacgaagc
     3121 accgcgcagg ccgccggccg attggtccgc gatgtcccct gggatgaggc cggtactccg
     3181 ccgccctccg ccgggaacgg cagcgccatg cgcgcgggac caattggcct tttcttccat
     3241 gacgatcccg agggactcgt gcgggctgcc acgcagcagg ggatcatcac ccatgcggac
     3301 ccgcgctgtt cggcgggcgc gatcgcaatc gccggagcgg tctccctcgt tctgagcgac
     3361 cggccggtcg atcccgcctt cctgctggac cggcttgccg ggcttaccgg ggaagtcgaa
     3421 ccacggttcg ccgccaactt gcgagagctc ctccgctggc tctcgctgcc gcccgaagag
     3481 gccgtgacgc gcatttcagg gacggggctc gatccggatc agggacgcga gtggcagggg
     3541 ataccgcctt tcgtcgtcag cagcgtcctg tggagcctgt actctttcct gaccagcccg
     3601 gaggattact ggcgcgcgat ccgcaccgct atcgccgtcg gcggagacgt ggacaccact
     3661 gccgcgatgg ccggggctat cagcggcgcg cacctcggtc tcgactcctt gcccggggac
     3721 ctcacccgcc acctcaccga ccggggtaca tggggcctcg cggacctcat ccggctggcc
     3781 gacgagtgcc attccgtccg ggttcggtga cgcgccggcg gtctcccgga gagagcggcg
     3841 agcgccgcgc cccggctgcg ggtacaatcg tcgcgtttgt gatggtggag cgcaacgctg
     3901 tgggaggttg atcctcgaca tgaaacccga gaccggagag atcctggtga gcgcggagca
     3961 gatagcggag aaggtgcgcg agatgggggc gcgtatcacc gaggactacc gcggagagcg
     4021 tccgctgctg gtaggcatcc tgcgcggggc ggtcgtcgtc ctcgcggacc tcatgcgcca
     4081 tatcgagttg cagtgcgaga tcgacttcat ggaagtcagt tcctacggta cgggaacctc
     4141 ctccagcggt gtcgtgcgca tcttgaagga cctggaagag gacctcaccg gacggcacgt
     4201 cctgatcgtc gaggacataa ttgacaccgg gcttaccctc tcgtacctgc ggcgctcact
     4261 attgacccgc aagcccgcgt ccctggagtt ctttaccctc ctctccaagc cttcccgccg
     4321 ccgcgtcgac ctcgatgtga agtacgtcgg cttcgaagtc cccgacgagt tcgtcgtcgg
     4381 ctacggtatc gactacgcag gatacttccg caacctgagc gatatctacg ccctgaaacc
     4441 cgacaccgcc atccctgaat aatccctcta actaatctcg gcgcagctcc ctgcgtctga
     4501 ccttcactgt ctcgcgctag accggagtca agacgctcga cggtccgaca cctgacccac
     4561 ctagatccac cgccgcgtcg ttgcggaaca tgcagtgggc atatgtctag aagtcatctc
     4621 acaaatacct cagcagtatg acgatgcagc ccatatgcac gaagccgagg tagttctgcg
     4681 ctcgccgctc gtagcgtact accagcc
//
LOCUS       DASSMB010000146        10028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1426814, whole genome shotgun sequence.
ACCESSION   DASSMB010000146 DASSMB010000000
VERSION     DASSMB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426814"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFJ72_08665"
     CDS             <1..271
                     /locus_tag="VFJ72_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7479569.1"
                     /translation="IVDIMLKRLNKHLESQRVAVEVTPAAKELLAEEGYDPKFGGRPL
                     ARAIRRYIENPLSSRIIGGEFGPGDTVVVDREGGELTFKAKVPAA"
     gene            complement(329..742)
                     /locus_tag="VFJ72_08670"
     CDS             complement(329..742)
                     /locus_tag="VFJ72_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7479570.1"
                     /translation="MERRVGYQLKRAQHALRGGMDEVLREIGMTTAQYAALSALEAEA
                     GLSGADLARRSFVTPQTMNQILTRLERDGMISRRAHPRHGRVLRTDLSKEGRRKLAEA
                     HKLVEEVEAKMLKNFDDAEIRQLSDALRRCAESLE"
     gene            839..1201
                     /locus_tag="VFJ72_08675"
     CDS             839..1201
                     /locus_tag="VFJ72_08675"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7479571.1"
                     /translation="MASVKGPDFVALQVRDLEASARFYEEKVGLERAPHGPSGAVVFR
                     TEPVAFAVREPLVDLDAVDRLGWGVALWMGCDDAQGLHDSLVEAGVPIAREPFDGPFG
                     RTFTFVDPDGYAITVHDG"
     gene            1194..2105
                     /locus_tag="VFJ72_08680"
     CDS             1194..2105
                     /locus_tag="VFJ72_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479572.1"
                     /translation="MAKVPRFELHPVGPYSLAASTKFLEDFTPAAYEGNEPGHLHMAF
                     VADGREEAAGVCLRSEGGAVVGEVYGEADFEVVRRQVERILSLDVDGSGFPEVGERDR
                     VVGRLQARYPGLRPVCFYSPYEAAAWALIGHRIRIVRAAQIKARMARELGSVVEIHGE
                     PERAFPGPARLARIEELPGLFGRKVEYLRHLGRAAMDGKLDAERLRSMPVSEALEELR
                     ELPGIGPFSAELILLRGAGEPDHLPANELRLGRAVAMAYGLDGAPKPEELEKVAERWR
                     PYRTWVSLHLRAMLEQETGEISRQKAN"
     gene            complement(2098..4200)
                     /gene="glgP"
                     /locus_tag="VFJ72_08685"
     CDS             complement(2098..4200)
                     /gene="glgP"
                     /locus_tag="VFJ72_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HET7479573.1"
                     /translation="MTSTIPSRLERLPELAQNLLWTWRPGIQKLFAGLDPDLWETTEH
                     NPVRLLNETSNLEAAAEDAAFIEDYQSALQDLDSYLDRRGTWMDRVYPDLGGPVAYFS
                     AEFGLHECLPIYSGGLGVLAGDHVKSASDLGVPLVGVGLLYSEGYFRQRLDAGGLQQE
                     VYEPFDPENRPVLPARDAGGREIHVTVPLAGREVRLKVWEVKVGRVSLLLLDANVPEN
                     AGGDRGITARLYGGDTHTRISQELILGVGGVRALRALDLHPAVFHMNEGHAAFSVLER
                     MRALVADGASFDEARERVYRDTVFTTHTPVPAGHDHFPRDLFEEYTAGWPEDLGTDRE
                     GLWNLGRKEEEWGETFNMTVLAMNLSAARNAVSELHRDVSLEMWEYLFSKNGRRPITH
                     VTNGIHTWSWLSPALYGLFERHSGGRAWRAAVEDASAWSFVEEIPDRDLWEAHRSEKR
                     AMTDFVNRRLQLQRERTGSSTPAQKLDPEALTLGFARRFATYKRATLLLHDAERLRLI
                     VKGADRPVQFVFAGKAHPADEPAKEFIRSLYGAAGEEDLAGHIVILEDYDMNVARHLV
                     QGVDVWLNNPRRPLEASGTSGQKASLNGAPNFSVLDGWWPEAYNGKNGWKIGEEKEYA
                     SQEEQDAADSESLYTTLEEALIPLYYTRDEAGVPGGWIEVMKEAISTVAPIFSTQRMV
                     QDYVHKLYVPRANGDGVS"
     gene            4592..6502
                     /locus_tag="VFJ72_08690"
     CDS             4592..6502
                     /locus_tag="VFJ72_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET7479574.1"
                     /translation="MKTLLAIPGRVIRNLRRGAAAYTVLIVALLLTALAWFYVREDVQ
                     AQNAVRFDETVNATQESINRRTDAYVDAMFGARGLFFASESVNSEEWKGYVNGIKPES
                     RFQGLQALGYAEYVTPEERASYFRRARENGQPGLRPDLDPGGEREGYYPVAFVEPLDD
                     ANRDLINRDEYTESQHRAAMDRARDTGEPVATSRVYVMTETAPGAVADLSMRPGFVVY
                     MPVYQKGEPVGTTGERRRALKGFVFGAFRMDGLLKSTFGGSFDPTLDFEVYDGAKTNE
                     SSLLYDDDGVRRAGMAREATKYQRSSRIKVAGREWTLFFSALPGFEQGADGGRFPAFL
                     RPLVENVGGTLPLFVLLSGLATSFLLFSIIWMLVRSRDRAERVSEELEHANRELEGAN
                     HELEAFSYSVSHDLRAPLRTIDGFSRILIEDYGDRLDAEGEDYLERVRGASQHMGSLI
                     DDLLDLSRVTRKPLRREAVDLSALATDIMERLKDSEPDRKVEVVVERGLIAQADANLL
                     SVALENLLGNAWKFTSKTPDARIEFRADRQPGPGFLAPVYYVRDNGAGFEMAYAQRLF
                     GAFQRLHGTEEFEGTGIGLATVQRIVHRHGGRVWAEGAVGEGATFYFTLGGARGGSGS
                     GAEAPAKKAEVA"
     gene            6499..>10028
                     /locus_tag="VFJ72_08695"
     CDS             6499..>10028
                     /locus_tag="VFJ72_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479575.1"
                     /translation="MSGPATHRGAFSEAPARRALRVLIVEDSENDAALLLRELRRGGY
                     EPDYERVDTPEDMERALAGEPWELVISDYFMPRFRAPEALALLRKLGHDTPFIVVSGK
                     VGEDLAVEAMRAGAQDYITKENMTRLNAAIERELREAEERRERRRAEEALSRSERRFQ
                     RLVEHAADAIFVYEPDGRFLDVNRKACESLGYTREELLQMSVPDIEVDYAPEELREIR
                     RRLTLGNPITIEGTQRRKDGTTFPVEVRVGLLESEGRKLILTLVRDITERKEAERKIR
                     ETETRYKALVEQIPAVTYIQDPETKQVRYVSPQIEDVLGYPPDREMLDDDHWIKIMHP
                     EDRERVIFEDERTDDTGEPFRMEYRQFARDGKMVWIRDQAILVRDEEGNPLYWQGVQY
                     DITDQKRAEEDLRASEERYRTFISQSTEGIWRIELAEPLSLDLSDGEMIEHAYRHGYL
                     AECNDAMARMYGYERAEDLVGTRLEDLLPLSEARNVDMLTRFARSGCRLTDAETREFD
                     REGNPRIFLNNLNGIVEGGMLVRIWGTQRDITERKLAEEELRRSENSLAEAQRIAHIG
                     NWDYDMARDEASWSDELYRIFGFAPGEFTPNYKSFLDLVHPDDRRIVRAEVRRAVYGG
                     DHSSIDYRVVRPDGETRYVNTRYEVVRDETGQPVSLTGTIHDITERKKIEDALREAEE
                     KYRGIFENSVEGIFQTTMDGRILTANPALARMLGYESPEEMVETVTDVSRQVYAERRQ
                     REEFLRVLRENETVSGFEIRALRKDGSEVLLSLNAHLVRDEAGEVVATEGSAENITER
                     RRAEETLRESEERFRATFEQAAVGFVQIGLEGEWIRFNDKFCEIAGYSREELAEISVF
                     DLISMEDFEEDFERGVQMLAGEIREYTQEKIIVGKDGRRVWINLTVSLVRDAVDAPRY
                     FIAVAEDISERKWAEEELRLRERAIDASINGIVLTDPNQPDNPIVYVNPAFERITGYS
                     GAEVIGRNCRFMQGGDRDQPALRELRAAIRERRECRVVLKNYRKDGTPFWNELSISPV
                     FDERRRLIRFVGVQDDITERRRVEEEQRLLAETGSVLASSLDYRTTLASVANLAVPGL
                     ADWCAIDVVEEDGSLNRLAVAHEDPEKIALAHELQLRYPPDPESSVGALQVVRSGQSE
                     LYSEIPDEMLIAAARDEEHLKLIREIGFTAA"
BASE COUNT         1744 a         3011 c         3569 g         1704 t
ORIGIN      
        1 gatcgtggac atcatgctga agcgcctgaa caagcacctc gaaagccagc gcgtcgccgt
       61 agaggtaaca ccggctgcga aggagcttct ggcggaagag ggttacgacc ccaagttcgg
      121 cggacgccca ctcgcccgcg cgatccggcg ctacatcgag aaccctctca gcagccgcat
      181 catcggcggt gagttcggcc ccggtgacac cgtggtcgtg gaccgcgagg ggggcgagtt
      241 gaccttcaag gcgaaggtgc ctgcggcgta aaagttccac aaaccaccaa ccagaagggc
      301 tacccgaatc gggtagccct tctctcgctt actccagcga ctcggcgcag cgccgtagcg
      361 cgtccgaaag ctgacgaatc tccgcgtcgt caaagttctt tagcattttg gcttcgacct
      421 cttcgacgag cttgtgcgct tccgccaact ttctccttcc ttctttggaa agatccgtcc
      481 gcagtacgcg cccgtgccgt ggatgagcac gccgcgagat catgccatcc cgctccagtc
      541 gcgtcagtat ctggttcatc gtctgcggcg ttacgaagct cctgcgcgcg agatccgccc
      601 cggataaccc cgcctccgcc tccagtgcgc tcaacgccgc gtactgggcc gtcgtcatcc
      661 cgatctcccg cagtacctcg tccataccgc cccgtaaggc gtgctgcgct cgtttgagct
      721 ggtacccgac cctccgttcc ataccctccg ctcgttccaa gaaaatgtcc cttgtgggat
      781 tatcagtgta ctgatataat atcagtgtgt tgatatagtt tactcgaaag gagcaggcat
      841 ggcgagtgtg aagggaccgg atttcgtggc gcttcaggtg cgggatcttg aggcgagcgc
      901 gcgtttttac gaggagaagg tggggttgga gcgggcgccg catgggccat cgggtgcggt
      961 ggtgtttcgc acggagccgg tggctttcgc ggtcagggag ccgctggtgg acctggacgc
     1021 cgtggatcgt cttggttggg gcgtggcgtt gtggatgggt tgcgacgacg ctcaggggct
     1081 gcacgactcc ctggtcgagg ccggtgtgcc gatagccagg gagccgttcg atggaccctt
     1141 cgggcggacg ttcacgttcg tggacccgga tggttatgcg ataacggttc acgatggcta
     1201 aggtaccgcg gttcgagcta catcccgtag ggccgtactc gcttgcggcg agcacaaagt
     1261 tccttgagga ctttactccg gccgcatacg aggggaacga gccgggtcac ctgcacatgg
     1321 ccttcgtggc ggatggtaga gaggaggcgg ctggtgtctg cttgcgctct gagggaggcg
     1381 cggtcgtcgg tgaggtgtat ggagaggccg acttcgaggt ggtgcggcgg caggtggagc
     1441 ggatactctc gctcgatgtg gacggcagcg ggttcccgga ggtgggggag agggaccggg
     1501 tcgttggccg gcttcaggcg cggtatccgg ggttgcggcc ggtgtgtttc tactcgccgt
     1561 acgaggcggc ggcgtgggcg ctcatcgggc atcgtatccg tatcgtgcgg gcggcgcaga
     1621 taaaggcgcg gatggcgaga gaattgggtt ctgtcgtgga aatacacggc gagccggagc
     1681 gcgctttccc cggtcctgct cgtcttgccc ggatcgaaga gcttccgggg ctcttcgggc
     1741 gcaaggtcga gtatttgcgc catctcggac gagcggcgat ggacggaaaa ttggatgcgg
     1801 agcgcctgcg ttcgatgcct gtctcggagg cgctggagga gctacgggaa ctgccaggga
     1861 tcggtccttt ttcggcggag ttgatccttt tgcgcggcgc gggcgagccc gatcatctac
     1921 cggcgaacga gctgaggctg ggccgggcgg tcgcgatggc ctatgggctc gacggagcgc
     1981 cgaaaccgga ggagctggaa aaggtggctg aacgctggcg accgtaccgt acctgggtta
     2041 gcctgcatct gcgggcgatg ctggagcagg agacgggcga gatctcccga cagaaagcta
     2101 actgaccccg tccccattcg ctcgcggcac gtagagcttg tggacgtagt cctggaccat
     2161 gcgctgtgtc gagaagatcg gggcgacagt gcttatggct tctttcatga cctcgatcca
     2221 gccgccgggg acgccggcct cgtcgcgggt gtagtagagc gggatcaggg cttcctccag
     2281 cgtcgtgtac agtgactcgg agtcggccgc gtcctgctct tcctgactgg cgtattcttt
     2341 ctcctcgccg atcttccaac cgttcttgcc gttgtacgcc tctggccacc atccgtcgag
     2401 gacgctgaag ttgggggcgc cgtttaagga ggctttctgg ccggaggtgc cgctggcctc
     2461 cagcgggcgg cgggggttgt tgagccacac gtccacgccc tgcaccagat ggcgcgccac
     2521 gttcatgtcg tagtcttcga ggatgacgat gtgtccggct aggtcttcct ccccggcggc
     2581 cccgtagagc gagcggataa actcctttgc cggttcgtcg gccgggtgcg ctttgccggc
     2641 gaagacgaac tgcacgggac gatccgcgcc cttgacgatg aggcgcaggc gctccgcgtc
     2701 gtggaggagc agggtcgccc gcttgtaggt ggcgaagcgg cgggcgaagc ctaaagtgag
     2761 tgcttcgggg tctagcttct gggctggggt ggagcttccg gtgcgctcgc gctggagttg
     2821 gaggcgccgg ttgacgaagt ccgtcatcgc gcgtttctcg gagcggtgag cctcccagag
     2881 gtccctgtcc ggtatctcct cgacgaagga ccaggccgag gcgtcctcga cggccgcgcg
     2941 ccaggcccgc ccgcccgagt ggcgctcgaa gaggccgtag agggcggggg agagccagct
     3001 ccaggtgtgg atgccgttgg tgacgtgggt gatgggcctt ctcccgttct tcgagaagag
     3061 gtactcccac atctccagcg agacgtcgcg gtgcaactcg gagacggcgt tgcgggcggc
     3121 ggagaggttc atcgccagta cggtcatgtt gaaggtctct ccccactcct cctccttgcg
     3181 gcctaggttc cagagccctt cgcggtccgt gcccagatcc tccggccatc cggcggtgta
     3241 ctcctcgaag aggtctctgg ggaagtggtc gtggccggcg gggaccgggg tgtgggtggt
     3301 gaagacggtg tcgcggtaga cgcgctcgcg agcctcgtcg aagctcgcgc cgtcggcgac
     3361 caacgcgcgc atgcgttcga ggacggagaa ggcggcgtgg ccctcgttca tgtggaagac
     3421 ggcgggatgg aggtccaggg cgcgcagggc ccggacgcct ccgacaccca gaatcagctc
     3481 ctgagagatc cgggtatggg tgtcgccgcc gtagagtctg gcggtgatcc cgcggtcccc
     3541 gcccgcgttc tcgggaacgt tggcgtcgag gaggagaagg gagacgcggc cgaccttcac
     3601 ctcccagacc ttcaggcgaa cctcgcgccc ggcgagcggg acggtgacgt ggatctcccg
     3661 gcccccggcg tcgcgggcgg ggagtacggg acggttctcc gggtcgaacg gctcgtagac
     3721 ctcctgctgc aagccgccgg cgtccaggcg ctgacggaag tatccctccg agtagaggag
     3781 gccgacgccg acgagcggaa cgcccaggtc cgaggcgctc ttcacgtggt cgccggccag
     3841 cacgccgagg ccgccggagt agatcgggag gcactcgtgc aggccgaact cggcggagaa
     3901 gtacgccacc ggtccgccga ggtccgggta gacgcggtcc atccaggtgc cgcggcggtc
     3961 gaggtaggag tccaggtctt gcagggcgct ttggtagtct tcgatgaagg ctgcgtcttc
     4021 ggcggcggct tcgaggttgg aggtctcgtt caggagccgg accgggttat gctcggtggt
     4081 ctcccagagg tccgggtcga ggccggcgaa tagcttctgt ataccgggac gccaggtcca
     4141 gagcaggttt tgggccagtt cggggagccg ttccagccgg gacgggatag ttgaggtcat
     4201 atagtgtgcc gttctgtcga gtgcaggaac gggtgcattc ttatttagaa accccggcgg
     4261 tgcaagcgcg cgggtgacaa acagggactt tagggtcgtg tggagccttg cgagcggcta
     4321 atgcgacgag taacatatcc atgtccggat gtttgggtcc gggcgagatc cggcggtttt
     4381 caacgggtcg agggttgcga ggaaggatgg ccgtggcacg ccgagggtgg ggatatgctt
     4441 ccgctacgcg acgcctgcat gatggcgccg cgtagtttgc gcttgtatcc ccctgtatcc
     4501 cggccaggag tttgcttgaa gattggtccg gtgaggctgg attgagagac gatacagagc
     4561 cgctccgcga gcgggtcatg acgggcgcgg tttgaagacg ctgctcgcca tacccggaag
     4621 ggtcatacgc aacctgcgcc ggggcgccgc ggcgtatacg gtgctgatcg tcgctctgct
     4681 gctaaccgct ctcgcgtggt tctacgtgcg cgaagatgtg caggcacaga acgccgtccg
     4741 cttcgacgag acggtaaatg ccacgcagga gtcaatcaac cgccgcacgg acgcatacgt
     4801 ggacgccatg ttcggcgccc gcggcctgtt cttcgccagc gaatcagtca actcggaaga
     4861 atggaagggc tacgtgaacg gcatcaagcc ggagagccgc ttccaggggc tgcaggcgct
     4921 cggctacgcc gagtacgtga ccccggagga gcgggcgagc tacttccgga gggccagaga
     4981 gaacggccag cctggcctgc gccccgacct ggaccccggt ggcgagcggg aaggttacta
     5041 tcctgtagcc ttcgtggaac ccctggacga cgccaaccgc gacctcataa accgcgacga
     5101 atataccgag tcccagcacc gggcggcgat ggaccgggcg cgcgacacgg gagagccggt
     5161 ggcgaccagc cgggtctacg tgatgacgga gaccgcgccg ggggctgtcg cggacctctc
     5221 catgcgtccc ggcttcgtcg tctacatgcc ggtctaccag aagggtgagc cggtcgggac
     5281 taccggggag cgccggagag cgctcaaggg cttcgtcttc ggagccttca ggatggacgg
     5341 cctgctcaag agcacgttcg gggggtcgtt tgatcccacg ctggacttcg aggtctacga
     5401 tggggcgaag accaacgaga gcagcctgct ctacgacgac gacggcgtcc ggcgcgccgg
     5461 gatggcgcgg gaggccacga aataccagcg gtcgagcagg ataaaagtcg ccgggcgtga
     5521 gtggaccctg tttttcagcg ccctgccggg ctttgagcag ggcgccgatg gaggccgatt
     5581 ccctgccttc ctccggccgc tggtggagaa tgtcggcggc accctgccgc tcttcgtgct
     5641 cctgagcggt ctggctacga gcttcctgct cttctctatt atctggatgc tcgtccgcag
     5701 ccgcgaccgg gcggagcggg tcagcgagga actggagcac gccaaccgcg agcttgaggg
     5761 ggccaatcac gagctggagg cgttctccta ctcggtctcc catgacctgc gcgctccctt
     5821 gaggaccata gacggtttct cccgcatcct gatcgaagac tacggcgacc ggctcgacgc
     5881 ggagggcgag gattacctgg agcgggtgcg cggcgcgagc cagcacatgg gcagtctcat
     5941 agacgacctg ctggacctct cgcgggtgac gcgcaagccc ctgcgccggg aggccgtaga
     6001 cctgagcgcg ctggcgacgg atataatgga gaggttgaag gattcggagc ccgaccggaa
     6061 ggtggaggtc gtggtcgaga gaggacttat agcccaggcg gacgccaacc tgctctcggt
     6121 ggctctggag aacctgctcg gcaacgcctg gaagttcacc tccaagaccc cggacgcccg
     6181 catagagttc cgcgccgaca ggcaaccggg accgggcttc ctggcgccgg tctactacgt
     6241 gcgcgacaac ggggccggct tcgagatggc ctacgcgcag cggctcttcg gggcgttcca
     6301 gcgcctgcac ggcacggagg agttcgaggg caccggcata gggctcgcca ccgtgcagcg
     6361 catcgtccac cgccacggcg gccgggtgtg ggccgagggc gcggtcggcg agggcgcgac
     6421 cttctacttc acgctgggcg gggcccgcgg ggggagcggg tcgggagccg aggctccggc
     6481 caagaaagcc gaagtcgctt gagtggaccg gccacacatc ggggcgcgtt ctccgaggcg
     6541 ccggcgcgcc gggcgctcag ggtcctgatc gtcgaggact ccgagaacga cgcggcgctc
     6601 ctcttgcggg aactgcggcg cggtgggtac gagccggact acgagcgggt ggacaccccg
     6661 gaggacatgg agcgcgcgct cgccggagag ccctgggagt tggtcatctc cgactacttc
     6721 atgccgcgct tccgggcgcc ggaggcgctc gcgctcttgc ggaagctcgg ccacgacacg
     6781 ccgttcatcg tcgtctccgg caaggtcgga gaggacctgg cggtggaggc tatgcgggcc
     6841 ggtgcgcagg actacataac caaagagaac atgacccgcc tgaacgcggc catagagagg
     6901 gagttgcggg aggcagagga gcgccgggag cggcgccgcg ccgaggaggc cctcagccgc
     6961 agcgagcgcc ggttccagcg cctcgtggag catgccgccg acgccatatt cgtctacgag
     7021 ccggacggcc gcttcctgga cgtcaaccgg aaggcctgcg agtcgctcgg ctacacccgc
     7081 gaggagttgc tccagatgtc cgtgccggac atagaggtcg actacgcgcc cgaggaactg
     7141 cgggagatcc ggcgaagatt gacccttggc aacccgatca ccatagaagg aacccaacgc
     7201 cgcaaggacg gtactacctt cccggtggag gtgcgggttg gtctgctcga gtccgaggga
     7261 cgcaagctca tactgaccct ggtgcgcgac atcaccgagc gcaaggaagc cgagcggaag
     7321 atccgggaga ccgagacccg ctacaaggcg ctggtcgagc agataccggc cgtcacctac
     7381 atccaggacc cggagaccaa gcaggtccgg tatgtgagcc cgcagataga ggacgtcctc
     7441 ggttacccgc ccgaccggga gatgctggat gacgaccact ggatcaagat catgcacccc
     7501 gaagatcggg agcgcgtgat cttcgaggac gaacgcaccg acgataccgg tgagcccttc
     7561 aggatggagt accggcagtt cgcccgcgac ggaaagatgg tctggatacg ggatcaggct
     7621 atcctcgtgc gcgatgagga gggcaaccct ctctactggc agggcgtcca gtacgacata
     7681 acggatcaga agcgcgcgga ggaggatctg agggccagcg aggaacgcta ccgcacgttc
     7741 atctcgcaga gcacggaggg tatctggcgc atcgagcttg cggagccgct atctctggac
     7801 ctctccgacg gggagatgat cgagcacgcc taccgtcacg gatacctggc cgagtgcaac
     7861 gacgcgatgg cgaggatgta cgggtacgag cgcgcggagg acctggtggg tactcgcctg
     7921 gaagacctgc tccccctctc ggaagcccgc aacgtggaca tgctcacgcg gttcgcgcgg
     7981 tccgggtgcc ggttgacgga cgccgagacc cgcgagttcg accgggaggg caaccccagg
     8041 atattcctca acaacctcaa cggcatcgtc gaggggggca tgctcgtccg catctggggg
     8101 acgcagaggg acataacgga gcgcaagctc gccgaggagg agttgcggcg cagcgagaac
     8161 agcctcgccg aggcgcagcg gatcgcccac atcgggaact gggactacga catggcccgg
     8221 gacgaagcct cctggtcgga cgagctctac cggatcttcg gcttcgcccc gggggagttt
     8281 actccgaact acaagagctt cctggacctg gtccatcccg atgaccggcg gatcgtccgg
     8341 gcggaggtgc gcagggccgt atacggcggg gaccattcga gtattgacta ccgcgtggtg
     8401 cggcccgacg gcgagacgcg ctacgtaaac acccgctacg aggtcgtgcg cgatgagacc
     8461 ggccagccgg tcagtctcac cgggacgatc cacgacataa cggagcgcaa gaagatcgag
     8521 gacgcgctgc gggaggccga ggagaagtac cggggcatct tcgagaactc cgtcgagggc
     8581 atcttccaga cgaccatgga cggccggatc ctcacggcta acccggcgct cgcccgtatg
     8641 ctgggctacg agtctcccga ggagatggtc gagaccgtca ccgacgtgtc ccggcaggtc
     8701 tacgccgaac ggcggcaaag ggaggagttc ctccgcgttc tgcgggagaa cgaaacggtc
     8761 tcggggttcg agatccgggc gctccgcaag gacgggagcg aggtcctgct ctcgctcaac
     8821 gcgcacctgg tccgggacga ggctggtgag gtcgtggcta ccgagggctc ggcggagaac
     8881 atcaccgagc gccgccgggc cgaggagacg ctgcgcgaga gcgaggagag gttccgggcg
     8941 acttttgagc aggccgccgt cggcttcgtg cagatcggcc tcgaaggaga gtggatccgt
     9001 ttcaacgaca agttctgcga gatcgccggc tatagccgcg aggagcttgc ggagataagc
     9061 gtcttcgacc tcatctcgat ggaggacttc gaggaggact tcgagcgcgg ggtgcagatg
     9121 ctggccgggg agatccggga gtacacccag gagaagatca tcgtcggcaa ggatggcagg
     9181 agggtctgga tcaacctgac cgtctcgctg gtgcgcgacg ccgtggacgc gccccggtac
     9241 ttcatagccg ttgcggagga catcagcgag cgcaagtggg ccgaggagga gcttcgtctg
     9301 cgcgagcgcg ccatcgacgc gagcatcaac ggcatcgtgc tcaccgaccc gaaccagccc
     9361 gacaacccca tcgtctacgt caacccggct ttcgagagga tcaccggcta ctccggcgcg
     9421 gaggtcatcg ggcgcaactg ccgcttcatg cagggcggcg accgcgacca gccggcgctc
     9481 cgcgagttgc gcgcggcgat aagggagcgg cgggagtgcc gggtggtgct caagaactac
     9541 cgcaaggacg gcacgccgtt ctggaacgag ctgtccatat ctccggtgtt cgacgagcgg
     9601 cggcgtctga tccgcttcgt cggcgtgcag gacgatatta ccgagcgccg gcgggtggag
     9661 gaggagcagc gtctcctggc cgagacgggt tccgtgctcg cttcctcgct cgattaccgg
     9721 accacgctcg ccagtgtggc gaacctcgcg gtgcccggcc tcgccgactg gtgcgccata
     9781 gacgtggtcg aggaggacgg ctcgctaaac cggctggcgg tggcgcacga agacccggag
     9841 aagatagccc tggcgcacga gttgcaactc cgttatccgc ccgaccccga atcctcggtg
     9901 ggggccttgc aggtggttcg gagcggccag tcggagctct actccgagat ccccgatgag
     9961 atgctgatcg ccgccgcgcg ggacgaggag catctgaagc taatacgcga gatcggcttc
    10021 acggcggc
//
LOCUS       DASSMB010000147        16803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1172629, whole genome shotgun sequence.
ACCESSION   DASSMB010000147 DASSMB010000000
VERSION     DASSMB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16803
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172629"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VFJ72_08700"
     CDS             <1..223
                     /locus_tag="VFJ72_08700"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7479576.1"
                     /translation="VWDLIRLAQSSIARRAIVPMQDVLELGSETRMNTPGTASGNWSW
                     RMDETALTPDLAERLRALSETYGRLETSE"
     gene            complement(457..1203)
                     /locus_tag="VFJ72_08705"
     CDS             complement(457..1203)
                     /locus_tag="VFJ72_08705"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET7479577.1"
                     /translation="MSRPKRWRIAYSSCAGRRLAGSPPEEKGSRENAKTKTLLACGII
                     AGPLFVLVFLLEGATRANYDPLRHPVSSLALGPSGWVQSANFVVSSLLTLAFAFGLRR
                     ALRPGKGSVWGPLLVGTWAAGLLGAGIFVTDPVSGYPPGTPDIASYTWHGAIHDLFSL
                     PAFVALTMACFVLARRFASQGERGWAVYSTVSGAVFVVAFVLASAGFGQAKNLVDLAG
                     LFQRAAVSVGFGWLTLLAWRLLKLTSQTSR"
     gene            complement(1139..1612)
                     /locus_tag="VFJ72_08710"
     CDS             complement(1139..1612)
                     /locus_tag="VFJ72_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7479578.1"
                     /translation="MEAAFEEARMLGHDSLGDEDLLLGILRADEGIASGALSSLGVTL
                     EEAREESQELFSEALYSIGISLEEVRREAGDAFDMCIPDDRKIPYSPRAKKALVQARK
                     EMRRLGDNHLGAEHVLLGILGNADGTAVRALGRLGVSAEALEDRVFELRGTEAGR"
     gene            complement(1648..1905)
                     /locus_tag="VFJ72_08715"
     CDS             complement(1648..1905)
                     /locus_tag="VFJ72_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479579.1"
                     /translation="MNEKTKTQVGGPEDPREGLEAVVALRRMLEALEAAQVENAFVAG
                     WSWARIAEVLGVSKQAVHKKHAGRLRVRYPGEPPKRKGRGE"
     gene            2094..2927
                     /locus_tag="VFJ72_08720"
     CDS             2094..2927
                     /locus_tag="VFJ72_08720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7479580.1"
                     /translation="MNYGFIITSGDPRAIAALAREAEVAGWDGVFYFDGICVGGMDTY
                     DPWVVMAAIAIETERVRIGAILTPPARRRPWKLARETMTLDHLSGGRLVLPVGLGALD
                     DGGFSKVGEPTDKKVRAGRLDESLEILTGLWSGKPFAYEGEHYRLEEMTFLPKPVQSP
                     RVPIWVVGAWPSRKSMNRALRYDGVLAAKAGGSATEPGVTPEDIRAMKEYVGENRAED
                     TPFDIVWEGVTPGDDPERAAAIVRPFAEAGATWWIESRWTPPNEPDDLRARIRQGPPR
                     L"
     gene            complement(2937..3521)
                     /gene="lepB"
                     /locus_tag="VFJ72_08725"
     CDS             complement(2937..3521)
                     /gene="lepB"
                     /locus_tag="VFJ72_08725"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7479581.1"
                     /translation="MSEGSSRGRDPREEKRKSASRNALEFAVTLLVAFVLVFGFIRPF
                     VVMAYSIPSVSMVPTLEVGDRVLVNKSIYRFKEPERRDIVIFDAVANGKPETLIKRVV
                     GLPGDRISLRDGILYLNGERQKEPYIVNKPCVRSLPKTCSFGPVTVPKDHIFVMGDNR
                     ANSEDSRFIGPVPRQSVKGEAFFRFWPPGRLGAL"
     gene            complement(3525..4184)
                     /gene="lepB"
                     /locus_tag="VFJ72_08730"
     CDS             complement(3525..4184)
                     /gene="lepB"
                     /locus_tag="VFJ72_08730"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7479582.1"
                     /translation="MSEKSHYEEQFQTRRERRESVRPKKGGGVLEFLIILIVAFTLVF
                     GFVRPYVVEAFWIPSESMVHTLEVGDRVLVNKFIYDFTEPKRGDIVVFKSVEEGPLPP
                     QQGLIQRITGFFTGQSAAAPRREELIKRVVGVPGDKISVQNGRLYVNNELQKEPYVNK
                     DLPDRSFFAPVTVPKNHVFCMGDNRANSRDSRFFGPVPDKNIEGEAFLLFWPLDRISL
                     L"
     gene            complement(4225..5637)
                     /locus_tag="VFJ72_08735"
     CDS             complement(4225..5637)
                     /locus_tag="VFJ72_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7479583.1"
                     /translation="MTPEQREKNRVTVVSLLAAVALTSAKLAAGLLTGSLGLLAEAAH
                     SGLDTVASVITFFSVRVAGRPADTNHPYGHGRFENLSAVIQGVLLLGTAAWILYESVQ
                     RIFFVHVEVQTSFWAFAVMGASIVVDFWRSRMLARAARKYNSRAIEADALNFRADMLS
                     SVVVLVGLSLSAYGAAVGSEGFLQRADAWAALLVGLFIIGKSGQLALRSVNILLDLAP
                     VELQERVTRAAESVPGVVGTRLVRLRESGDRKFADIVVTVPRTLSAAEAHEISERVEE
                     SVRDIDPGTESVVHTEPVMSASETAAESIHAVALGMGLRTHHERVQREGDRLEASLHL
                     EVDSSLTLGEAHDLARRLGTAIKSQNPSLRRVNTHIEVAEPEPGERLEVTADHGDLAR
                     EMKREVREADADAVCHEVRLYRSAEGGLDAVLHVDFPRSANVGEVHIRTEAIEQALRE
                     AHPQLEHVVIHAEPREETAP"
     gene            complement(5692..6459)
                     /locus_tag="VFJ72_08740"
     CDS             complement(5692..6459)
                     /locus_tag="VFJ72_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7479584.1"
                     /translation="MDFFQLLFLGVIGVVGGMLAGLVGVGGGIVFVPGLVYVGGWSIK
                     EAVAASLVIIIFSSISGTIRNSKSEDPVNWRVAALLSLAVAPASLIGVAVSRVSSDTV
                     VEIVFSIVLLLLAYPTARGRPDFDESKKIPLIWVFVAGVFIGALSGLVGVGGGVVMVP
                     LMVLGMGIGTKQAVSTSLAVIMFTGVVGATGYIVTGFRGAEELLSLPPLIIGSMIGAW
                     IGVKIRDPLPADTIRIGFAVFMAVTALRLLTDALGIF"
     gene            complement(6487..7107)
                     /locus_tag="VFJ72_08745"
     CDS             complement(6487..7107)
                     /locus_tag="VFJ72_08745"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7479585.1"
                     /translation="MLEAILQPLIDFVTNTIGSYGAPAVFVLMLLESMGILIPSEAIS
                     PFAGYLVSQEKMTLLAAITAGVLGNLVGSWIAYFIGLWGGRELWFHYGKYVGVRAHHL
                     HVAEKWFEKYGEFTVFVARCLPVVRTFISFPAGTARMNFGKFTFYTLLGCVPWVSALT
                     YFGYVLGENWSEVGHYLHYLDYAVAAAVLAGAVYLFWRWRRSRSAA"
     gene            complement(7149..7787)
                     /locus_tag="VFJ72_08750"
     CDS             complement(7149..7787)
                     /locus_tag="VFJ72_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7479586.1"
                     /translation="MFGNLGGWVADVLAASGSLGLAFLLLLENLFPPIPSEVILPLAG
                     FLIWRGDLGAPQALVAATAGSLLGAYALYAIGRWGGRPLVLRFGRVLRVTERDLDRAE
                     GWFDRYGSVVVFVARMMPGARSIVSIPAGMLHMPLARFTLLTALGSAVWNALLLTAGY
                     ALGDNWSRVSDVVGPLSNAIVLVILISAAVLIVWWVYFRKREKRRKRKRRGE"
     gene            complement(7777..8841)
                     /locus_tag="VFJ72_08755"
     CDS             complement(7777..8841)
                     /locus_tag="VFJ72_08755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7479587.1"
                     /translation="MQSSQIPLREPYVQSVTTSSAVISWESKAPGIGKIEYGPHGSLN
                     RDKSDETNGRQHSVKLSGLRPDSIYYYRVGKSAETASFRTAPERGATGFTFAVVGDSG
                     DGGADQEAVAGLLRRMDPALILHTGDVVYDSGAMEDYGPRFFEPYSKLISETPIFPSL
                     GNHDVKTADGAAYLKNFHLPDNNSPHTERYYSFDWGNAHFVALDSELYYDDGSSSPQE
                     QKSWLEKDLAAAGGKWKFVFFHRPPYSSSEHGSDERIRADLAPIFERYGVNVVFNGHD
                     HDYERTVPIRGVTYVVTGGGGKDLYAAGESEWTAFSKSVHHATKVQVEGDRLTLEAVE
                     PDGTIIDRVKLDRRDTASVR"
     gene            complement(8934..10760)
                     /locus_tag="VFJ72_08760"
     CDS             complement(8934..10760)
                     /locus_tag="VFJ72_08760"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7479588.1"
                     /translation="MNTIENILSLIAQYGYLIVLFGVMLESMGIPLPGETILISAGIL
                     VQQGRLDLGDAILFGIMGAVIGDQIGYWIGRRGGRPFILRWGRYVLITPERLARAEAF
                     FARHGGKAVFLARFVTGLRVFGALVAGVSRMRWRTFFLYNALGGATWATAAVLVGYLL
                     GGSVGLVEKWIGRASALLAVALVAAIALYLAYRWVRNHPELVRRTAVRLGGRRVLRFL
                     DTPAGMWLRRRLAPGEAYGLAFTAGLVLFGLFSWAFGGITQDVLARDPLVRADAAILR
                     FFHSHGDPRLTAAVSVFETVFSPESMLLAGALAGAGLLFAGRRSREFEKSFAGAVLLA
                     TSFGTGALVELFKHLFHRPRPPASLQFVAETGNSFPSGHAMTALVVGATVWYLFSIRP
                     TCSRWGSWQAKARIGALAAAFALVVGVGRVYTGAHYPSDVLAGWALGGVWASICLTAA
                     EVFRGLRASGKPLTEPLLETAVRYAQFSFVGALNAAVDLAALNLFLILDPTREPGQLV
                     LYNAAALVLANANSYLWNTLWTFRHHARHDGRQVGMFVAQAVLNVGVGSLVFYLCAHL
                     LAGYSGLSPFVGGNAAKIVSMLTASTMSFLILRHLIFHKEKV"
     gene            complement(10889..11560)
                     /locus_tag="VFJ72_08765"
     CDS             complement(10889..11560)
                     /locus_tag="VFJ72_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7479589.1"
                     /translation="MRGVFREWFLSNDAGWDPRSYERYFGTPLGAAFRDREERVIFEF
                     VGNLMRPRHSVLEVGCGTGNYTVPLARRCAEITAVDSSPEMRGYLRERLIRERVTGVE
                     IRPGRLPDDLGALRGFDGVLSVGVLNYVEDLAASLRSLAAPLKPGGWIVFTVPLRSVE
                     GGIYAAAELANRRRIYPRTFKEVSALTGRAGLKILESAAAGLTRSGLTLVVAAVERAG
                     APGAS"
     gene            complement(11582..12415)
                     /locus_tag="VFJ72_08770"
     CDS             complement(11582..12415)
                     /locus_tag="VFJ72_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479590.1"
                     /translation="MLLGVALILASTFAYNGSAVLLATETRRHAGDSALLLAVGRQAS
                     GAFAISMNLLGWALEVAALTLIPLTLARVLNAAGLVVLLGMTRWALKESFGRREALGV
                     GLVALGIVAVGYAPPDTGNSSPGFPQYVLLFALLAPGILAPGALRLLRRSPGAALTAT
                     AAGLAYAMSGILNKSVAHDLHVSKIPSLALLMLCIVALGLWGFATELNALRDGRVSVV
                     VPIVLALHTVVPIVFAPFLFGESWPTELFARVILGGGIALTILGTLALTGSGNREPDG
                     S"
     gene            complement(12406..13122)
                     /locus_tag="VFJ72_08775"
     CDS             complement(12406..13122)
                     /locus_tag="VFJ72_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7479591.1"
                     /translation="MRIAGPALISALLAAHAAPASALTTTGRLFFPAITQVRASRRAV
                     ALTFDDGPDACLEAFLGLLEEYEVRATFFAVGEQVERDPGRLKETFARGHEVAVHCYR
                     HRNHLRLTPRQTIEDMRRARAVIEETTGAPTCLFRPPYGVFNAASWIEAGRQGWERVL
                     WARWGRDWEARATPRSVADEIGLPAVGDIVLLHDSDRYAAPGSWRATLGALPRILERL
                     DSMGLAACSVGDLLDSEECF"
     gene            complement(13098..14912)
                     /locus_tag="VFJ72_08780"
     CDS             complement(13098..14912)
                     /locus_tag="VFJ72_08780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7479592.1"
                     /translation="MIDYLIVALAVFLLWTGLVRFRRTARESYLPDAHPEKILILTAA
                     VGGGHEAAGRALRDELERTGHTVEVADGLKAMSRGLDWLLTRFYCNQVRSTPRSLAAV
                     FAVTSWTMGAWTIRSLVGLLFSHRLLDLVNERRPDLVISTYPLVNAALGRLRANGALK
                     VPAVAVIADYGVHPLWVAPGLDLHLVVSGPSAQLTELAGGSASVIRMPVAPAFRSALP
                     HDEARAALDIPQSAFVALVVGGAWGIGDLEGAARRAAEAGVYAVVVTGENSDLKERLE
                     KSFEREENVRILGWSKDLPALMSAADCMIQNAGGMTCLEAVQMGLPIVMFDPIRGHGE
                     LNCRVMERAGVVRRARDGEDLTALLRSAATGAMWLPAPEKTSAPGIAIALEAVSGSVP
                     RPVARRRVPQFRPALAGAAAAAFFFWSAFASSGVAVASRAFRFDVPGYDPAPGRVALA
                     VKVDDPKTARAVEDFAQHERAPMTIFSGARGAGGLHPAPGLTFGVAEEAGSRRIPVPM
                     RDRSEDREAAAALQRATGEYPGYFLPSPKTDLAALADAPPHTRLVMAERDTAGGPRAG
                     VLVVDSSGLSPEVARSRLADALRQVHHKGLQCVSPDRL"
     gene            complement(15047..16480)
                     /locus_tag="VFJ72_08785"
     CDS             complement(15047..16480)
                     /locus_tag="VFJ72_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7479593.1"
                     /translation="MPIRWRLTIFNALAIGLILLFLGLALFLLLRNALFANIEETVRG
                     EAVAAARSVEAGDGLGEAGRLAPNEVYLMVRDKRGQILDLTDNFPDNGKISDRVWRRA
                     LESGKPVYGKAEISHEEGADYVYAVPVKPKTGTARVVEAGKSSREVENTLEVFDALLI
                     IVIGAAFLVSLVGAYLLARAALRPVETVVGSAREITESDLAKRLPVSNPRDEIGRLAV
                     TINALLSRLEAAFARREEALSRQRRFAADASHELRTPLTSISGYARMLDEWGLEDPTV
                     ARESITAIRNETDRLRALAESLLALTRGDEGPPMRVEPADLATVAAEAAETARTIVGD
                     RLKVEYDQPDGPVEATFDRNRVRQVAAILLDNAIKYTPDGGRVTVAARERGGWAELVV
                     SDTGIGIPEEQLPLIFERFHRVDAARAGQGPSAGGAGLGLSIARQIAEAHGGTIEAES
                     QPGKGSIFTLKIPGNWRRIRPEAESAV"
     gene            complement(16480..>16803)
                     /locus_tag="VFJ72_08790"
     CDS             complement(16480..>16803)
                     /locus_tag="VFJ72_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7479594.1"
                     /translation="YDGEEVLRVADLRINTATREVRRGEGSTEREIDLTNREYELLEF
                     MARNHRRVLSREVLLSRVWDDEFGVGTNVVDVYVGYLRKKVDLPGESRLIQTVRGAGY
                     ALRER"
BASE COUNT         3001 a         5697 c         5466 g         2639 t
ORIGIN      
        1 ggtgtgggac ttgatccggc tcgcccaatc ctccatcgcc cgccgcgcca tagtccccat
       61 gcaggatgtg ctggagctag gctccgagac ccggatgaac acccccggca ccgcgagcgg
      121 caactggtcc tggcgcatgg acgaaacggc cctcaccccg gatcttgccg aacggctgcg
      181 cgcgctctct gagacttacg gccggttaga aacttcagag tagcagctct ctgatctcgc
      241 catcaggcgt cacgaaacac ctgccgtcga gcccgctagc cgtccgctcg ctcgcgcagt
      301 tcgaacagtc ggccttccag ggtctccacc gccacgccca tgcggaacag catcctgatg
      361 gcgcgctcat caaggaatgt ccggttcatg gcggggtcac agatagcaca aaggcgtccg
      421 attgcggcga gtggggacgg gcgagtggat cggcgtctac ctcgatgttt gggatgtgag
      481 cttaagcagg cgccaggcca gcagggtaag ccaaccgaag ccgacgctga ccgcggcgcg
      541 ctggaacagg ccggctaggt ctaccaggtt cttggcctgg ccgaagcccg cgctggcaag
      601 cacgaatgcg accacgaata cggcaccgct gacggtcgag tagaccgccc accctctctc
      661 gccttgtgag gcaaaccggc gagcgagcac gaagcaggcc atcgtcaaag ccacgaaagc
      721 agggagcgag aagaggtcgt ggatggcgcc gtgccaggtg taagaggcga tgtcgggggt
      781 tccgggaggg tagccgctca cagggtcggt cacgaaaatg ccagcgccga gtaggcccgc
      841 agcccaggtc ccaaccagca acggccccca gaccgacccc ttccccggcc gaagcgcacg
      901 ccgcagtccg aaggcgaaag cgagcgtcag gaggctggag acgacaaagt tggcactctg
      961 cacccacccg gaggggccaa gcgcgagcga gctgaccgga tggcggagcg ggtcgtagtt
     1021 cgcgcgggtg gctccctcga ggaggaacac caggacgaac agcgggccag cgattatgcc
     1081 gcaagcgagg agtgtcttcg ttttcgcgtt ttcacggctt cctttctctt ccggcggact
     1141 accggccagc ctccgtcccg cgcagctcga atacgcgatc ctccaacgct tcggccgaga
     1201 cacccagacg gcccagcgcc ctgacggccg tcccgtccgc gttccccaag attcccaaca
     1261 acacgtgctc ggcgcccaaa tggttgtctc cgagcctccg catctccttg cgggcctgca
     1321 ccagcgcctt cttcgcccga ggcgagtacg ggatcttgcg gtcgtccggg atgcacatgt
     1381 cgaaggcgtc tccagcctcc cggcgcactt cttcgagtga aataccaatc gaatataggg
     1441 cttcggagaa cagttcttgg gactcttctc gcgcttcttc gagcgttacg cccagcgagg
     1501 acagcgcccc ggaggcgata ccctcgtcgg cgcggagtat ccccagcaga agatcttcat
     1561 cgccgagcga gtcgtggccc agcatcctgg cttcctcaaa ggctgcttcc acacatctac
     1621 gcgacttatc ggtgagccgg ttgaagatca ctcgcctctc ccctttctct tcggcggctc
     1681 tccgggatac ctgaccctaa gtctcccggc gtgctttttg tgtacggcct gcttgctaac
     1741 gcccagcacc tcggcgatcc gcgcccacga ccaccccgca acgaaggcgt tctccacctg
     1801 agccgcctcc aatgcctcca gcatccgcct caacgcaacg acggcctcaa ggccctcccg
     1861 cgggtcctcc ggtccaccaa cctgtgtctt cgtcttctcg ttcatgcgtc aactatagtt
     1921 gaccaaatag taaatgtcaa cctttattga cgaaatttcg gcagcttgta ggagagaagt
     1981 aaagtctctg tcggagtcgg tatcccgtcg cgcaagaccg gcactttggg ggtggagcgg
     2041 tccgctgtac gattggctgg gaagggtgga tactgcgagg agaggaggca tgcatgaact
     2101 atggattcat catcactagt ggagatccca gggccatcgc ggcgctcgcc cgcgaggcgg
     2161 aggttgcggg ttgggacgga gttttctatt tcgacggcat ctgtgtcggg gggatggaca
     2221 cctacgaccc gtgggtggtg atggcggcga tagctattga gaccgagcgg gtccggatcg
     2281 gcgccatact cacgcctccg gcccggcggc gtccctggaa gctcgcccgc gagacgatga
     2341 cgctggacca tctctcgggc ggacgtctgg tgttgccggt agggctgggt gcgctcgatg
     2401 atggcggttt ctcgaaggtc ggggagccta cagacaagaa agtgcgggcc ggacggctgg
     2461 acgagagcct ggagatcctc accggtctgt ggagcggaaa accgttcgcc tacgagggcg
     2521 agcactaccg tttggaggag atgaccttcc tgccgaagcc cgtgcagtcg ccgcgggtcc
     2581 cgatctgggt ggtcggagct tggccgagca ggaaatccat gaaccgcgcg ctgcgctacg
     2641 acggcgtgct ggcggccaag gcaggcgggt ccgctaccga acctggcgta acgcccgagg
     2701 acatccgcgc gatgaaggaa tacgtcgggg agaatcgggc ggaggatact ccgttcgaca
     2761 tcgtgtggga aggggtgacg ccgggcgatg acccggagcg ggcagccgcc atcgtgcggc
     2821 ctttcgccga ggcgggcgcg acgtggtgga tcgagtcgcg gtggacgccg ccgaacgagc
     2881 ccgacgacct gcgcgcccgc atccggcagg gtccgccgcg cctctgaagc cggtggctag
     2941 agcgcgccca ggcggcccgg cggccagaag cggaagaacg cctcgccctt cacactctgg
     3001 cgcggtaccg gcccgataaa gcgcgagtcc tcggagttgg cgcggttgtc gcccataacg
     3061 aagatatggt ctttggggac ggttacgggg ccgaaggagc aagtcttagg caggctgcgg
     3121 acgcagggct tgttgacgat gtagggctcc ttctggcgct cgccattcag gtagaggatg
     3181 ccgtcccgga gcgagatcct gtctcccggc aaacccacga cgcgcttgat gagtgtctcc
     3241 ggcttcccgt tggcgacggc gtcgaagatc acgatgtcac gccgctccgg ttccttgaaa
     3301 cggtagatgg acttgttgac cagcacccgg tctccgacct ccagcgtcgg gaccatgcta
     3361 accgagggga tgctgtaggc cataaccacg aacgggcgaa tgaacccgaa taccagtacg
     3421 aaggctacga ggagcgtgac cgcgaactcc agcgcgttgc gcgaggcgct cttgcgtttc
     3481 tcctcgcggg ggtcccggcc tcgtgaggag ccctcgctca tccgctagag gaggctaatg
     3541 cggtccagag gccagaacag caggaaagcc tcaccctcga tgttcttgtc ggggacgggt
     3601 ccgaagaagc gtgagtcgcg ggagttggcg cggttgtctc ccatacagaa gacgtggttc
     3661 ttcggtaccg tcacgggcgc gaagaagctc cggtccggca ggtccttgtt gacgtagggc
     3721 tccttctgga gctcattatt cacgtagagc cggccgttct ggacggagat cttatccccc
     3781 ggcactccga cgacgcgctt gatcagctcc tccctccgcg gggcggcggc gctctgtccg
     3841 gtaaagaagc cggtgatgcg ttggatcaga ccctgctgcg gcggtagcgg gccttcctcg
     3901 acgctcttga agaccactat gtcaccgcgc ttcggctcgg taaagtcgta gatgaacttg
     3961 ttgaccagga cccggtcgcc cacttccagg gtatggacca tgctctcgga ggggatccag
     4021 aaagcctcca ccacataagg ccgcacgaag ccgaagacca gcgtgaacgc gacgatcagg
     4081 atgatcagga actcgaggac tcctccgccc tttttcggac ggacggactc gcggcgctcc
     4141 cggcgggtct ggaactgctc ctcgtagtga gacttttcgg acatggacgc taagaatacc
     4201 acggggtgtg tggcaagggc tccgttacgg cgcggtctcc tcccggggct cggcgtggat
     4261 gacgacgtgc tccagttgcg ggtgcgcctc cctgagcgcc tgctctatgg cctcggtgcg
     4321 gatatgcact tcgcccacgt tcgccgatct cgggaagtcc acgtgcagca ccgcgtccag
     4381 cccaccctca gccgagcggt agagccgcac ctcgtggcag accgcgtccg catcagcctc
     4441 gcgcacttcc cgcttcatct cccgcgcaag gtccccgtgg tccgccgtga cctccaggcg
     4501 ctcgccgggc tccggttcgg ccacctcgat gtgcgtgttc acccgcctca gggacgggtt
     4561 ctgggacttg atcgccgttc ccaggcgacg ggcgaggtcg tgcgcctctc ccagcgtgag
     4621 cgacgaatcc acctccaggt gcagcgaagc ctccagacgg tcgccttccc gctgaacccg
     4681 ctcgtggtgc gtgcggaggc ccatcccgag ggcgacggcg tggatggact cggcggcagt
     4741 ctccgacgcg ctcatcacgg gctcggtgtg gacgacgctc tccgtgccgg gatctatatc
     4801 ccgcacagac tcctctaccc tctcggagat ctcgtgcgcc tcggcggcgc ttagagtgcg
     4861 cggcacggta acgacgatgt cggcgaactt gcggtccccg gactccctga gccgcacaag
     4921 ccgcgtcccg acgacccccg gcaccgactc ggccgcccgc gtcacccgct cctgaagctc
     4981 cacgggcgcg aggtcgagca ggatgttgac cgagcgcagg gcgagctggc cgctcttgcc
     5041 gatgatgaac agcccgacaa ggagcgccgc ccaggcatcc gcccgctgca ggaagccctc
     5101 gctaccaacg gcagccccat aagcggagag cgagaggccc acgagcacga ccaccgagga
     5161 gagcatgtcc gcccggaagt tcagggcgtc ggcttcgatg gcgcgactgt tgtacttgcg
     5221 ggcggcgcgc gcgagcatcc gggagcgcca gaagtccacg acgatgctcg cccccatcac
     5281 ggcgaacgcc cagaacgagg tctgcacctc tacgtggacg aagaagatcc gctgcacgga
     5341 ctcgtagagg atccaggccg ccgtccccag tagcagcacg ccctggatga ccgccgagag
     5401 gttctcgaac cggccgtgcc cgtatgggtg gttcgtgtcc gccggacgcc ccgcaacccg
     5461 caccgagaag aacgtgatga ccgaggccac cgtatccagc ccggagtgcg ccgcctccgc
     5521 cagcagaccg aggctgccgg tgagcagccc ggcggcgagc ttcgccgagg tgagcgcgac
     5581 cgccgctaag agcgagacga cggtcacgcg gttcttctcc cgctgttcgg gggtcatctt
     5641 ttgagatcta gcctgcatat cccgaatcta ccatgcgccg cagcgtctga gttagaagat
     5701 gcccagagcg tcggtgagca agcggagagc cgtgaccgcc atgaaaacgg cgaacccgat
     5761 ccggatcgtg tctgccggca acgggtcccg gatcttcacc ccgatccagg ccccgatcat
     5821 cgagccgatg atgagcggcg gcagactcaa tagctcctcc gccccccgga agccggtgac
     5881 tatgtagccc gtggccccca ccacccccgt gaacatgatg accgccaggc tggtcgagac
     5941 cgcctgcttc gtgcctatcc ccatcccgag caccatcagc ggcaccatca cgaccccgcc
     6001 gccgacgccg acgagccccg agagcgcccc gatgaaaacc cccgcgacga agacccagat
     6061 gagcggtatc ttcttcgact cgtcgaagtc cgggcgtccg cgggccgtcg ggtaggccag
     6121 gagcaagagc actatggaga agacgatctc gaccaccgtg tccgaggaga cgcggctgac
     6181 ggcgacgccg atgagcgacg cgggagccac ggccagcgag aggagcgcgg cgacccgcca
     6241 gttgacggga tcctcactct tcgagttgcg gatagtgccc gagatggagc tgaagataat
     6301 gatgaccagg ctcgcggcca cggcctcctt gatgctccaa ccgccgacgt acaccagacc
     6361 cggtacgaag actatgccgc cacccactcc cacgagcccg gcgagcatcc ccccgaccac
     6421 cccgatgacg cccaggaaca gtagctgaaa aaagtccatc gccctattct agcggagcga
     6481 cgctctctag gccgccgagc gggagcggcg ccagcgccag aacaggtaca cagcgccggc
     6541 cagcaccgcc gcggcgaccg catagtccag gtagtgcagg tagtgcccga cctcgctcca
     6601 gttctctccc agcacgtacc cgaagtacgt cagcgcggag acccacggca cgcaaccgag
     6661 cagcgtgtag aaagtgaact tgccgaagtt catccgggca gtcccggcgg ggaatgagat
     6721 aaacgtcctg accaccggca ggcagcgcgc gacgaagacc gtaaactcgc cgtacttctc
     6781 gaaccacttc tccgcgacgt gcaggtggtg cgcccgcacc ccaacgtact tgccgtagtg
     6841 gaaccacagc tcgcgcccgc cccagagccc gataaaatag gcgatccacg agccgaccag
     6901 gttgccaagt acccccgctg tgatcgccgc cagcagcgtc atcttctcct gcgagacgag
     6961 gtagccggcg aagggcgaga tggcctccga ggggatcagt atccccatgc tctccagcag
     7021 catcagcacg aagaccgccg gcgcgccgta gctcccgatg gtattcgtga cgaagtctat
     7081 gagcggttga aggatcgctt cgagcacgag gggaagctac cacctaccgg ccgggttttc
     7141 cagtgacact actcgccccg ccgcttgcgc ttccggcgct tttcgcgctt cctgaaatac
     7201 acccaccaca ctatcaggac tgcggccgag atcaggatca cgagaacgat ggcgttggag
     7261 agcggcccca cgacgtccga gaccctggac cagttgtcgc cgagagcgta ccccgctgtg
     7321 agcagcagcg cgttccacac cgcggagccc agggccgtta gtagcgtaaa gcgcgcgagc
     7381 ggcatgtgca gcattccggc cggtatggag acgatactgc gggcgccagg catcattcgg
     7441 gcgacgaaga caaccacgct cccgtacctg tcgaaccatc cctcggcgcg gtccaggtcc
     7501 cgctccgtaa cacgcagcac gcggccgaag cgcaggacga gcgggcgtcc gccccaccgg
     7561 cctatcgcgt acagagcgta ggcgccgagc agggaacccg ccgtggccgc cacgagcgcc
     7621 tgcggagcac cgaggtctcc ccgccagatc aggaacccgg cgagaggcag tattacttcc
     7681 gaaggtatcg gcggaaagag gttctcaagc aggagcagga acgccagacc caacgagccc
     7741 gaagccgcga gcacgtccgc gacccatccg ccgaggttac cgaacactgg ccgtatccct
     7801 ccggtcgagc ttcacgcggt cgatgatcgt gccgtccggc tcgacggctt cgagcgtgag
     7861 ccggtctccc tctacctgca ccttcgtggc gtggtggacg gacttcgaga acgcggtcca
     7921 ctcgctctct ccggccgcgt agaggtcctt gccgccgccc ccggtcacga cgtaggtgac
     7981 gccccggatc gggacggtgc gctcgtagtc gtggtcgtgg ccgttgaaga cgacgttcac
     8041 gccgtagcgc tcgaagatgg gcgcgaggtc ggcccgtatc ctctcgtcgc tgccgtgctc
     8101 ggaggagctg tacggcggcc ggtggaagaa gacgaacttc cacttccccc cggcggccgc
     8161 gaggtccttc tccagccacg acttctgctc ctgtgggctg ctactcccgt cgtcgtagta
     8221 cagctcgctg tcgagggcta cgaagtgggc gttcccccag tcgaaggagt agtagcgttc
     8281 ggtgtgtggc gagttgttgt ccgggaggtg gaagtttttc aggtacgcgg cgccgtccgc
     8341 ggtcttgacg tcgtggttgc cgagggatgg gaagatggga gtctcgctta tgagcttgct
     8401 gtatggctcg aagaaacgcg gtccgtagtc ttccatcgcg ccgctatcgt acaccacgtc
     8461 gccggtgtgc aagatcaggg ccgggtccat tcgccggagc agcccggcga ccgcctcctg
     8521 gtccgcgccg ccgtctccgc tgtccccgac gacggcgaag gtgaagccgg ttgcgccacg
     8581 ctcgggagcc gtgcggaagg aagcggtctc cgcggacttc cccacccggt agtagtagat
     8641 cgagtccggg cgcaggccgg agagcttcac ggagtgctgt ctcccgttcg tttcgtcgct
     8701 cttatcgcgg ttgaggctcc cgtgaggacc gtattcgatc ttccctattc cgggggcttt
     8761 actctcccag gagatcacgg ccgagctggt cgtcacgctc tgcacgtaag gctcgcgcag
     8821 cggtatctgc gacgattgca cctccgtctt cgcggacggg ctctccgagg agcatccggc
     8881 gagcagcagc gccgccagga ctgtcagagc cagcagcgcg gtgggcgcgg ctctcagacc
     8941 ttctccttgt ggaatatgag gtggcgcagg atcaggaagc tcatcgtcga ggcggtgagc
     9001 atggagacga tcttggccgc gttacccccg acgaacggcg agaggccgga gtacccggcg
     9061 agcaggtgcg cgcagaggta gaagaccagg ctccccacac ccacgttgag cacggcctgc
     9121 gcgacgaaca tgcccacctg ccgaccgtcg tggcgcgcgt ggtggcgaaa ggtccacagg
     9181 gtgttccaga ggtagctgtt cgcgttcgcc agaacaagag cggcggcgtt gtacaggacg
     9241 agctggccgg gctccctggt ggggtccagg ataaggaaca ggttcagcgc cgcgaggtcc
     9301 acggccgcgt tgagggcgcc cacgaaggag aactgcgcgt accggacggc cgtttcgagc
     9361 agcggctcgg tgagcggttt gccgctcgcc cgcagcccgc gaaagacctc ggcggccgtc
     9421 aggcagatag aggcccacac cccgcccagc gcccatcccg ccagcacgtc gctcgggtag
     9481 tgcgcccccg tgtagacgcg ccccacgccg accaccaggg cgaacgcggc ggccagcgca
     9541 ccgatccggg ccttcgcctg ccaggagccc cagcggctac aggtaggacg gatgctgaag
     9601 aggtaccaga cggtggcgcc cacgacgagc gccgtcatgg cgtggccgct cgggaaacta
     9661 ttgcccgttt cggccacgaa ctgcagcgaa gccggcggtc gcggccggtg aaagaggtgc
     9721 ttgaagagct ccacgagcgc cccggtcccg aaggacgtgg cgagcaggac cgccccggcg
     9781 aagctcttct cgaactcccg gctccggcgc ccggcgaaca ggagccccgc gcccgccaga
     9841 gcccccgcca agagcatcga ctcgggagag aagaccgtct cgaacacgct caccgcggcc
     9901 gtcagtcgcg gatcgccgtg ggagtggaag aagcgcagga tggccgcgtc ggcccggacc
     9961 agggggtcgc gggccagcac gtcctgcgtg atgccgccga aggcccagga gaagagcccg
    10021 aagagaacga gaccggcggt gaacgcgagt ccgtaagcct ctccgggcgc cagcctccgg
    10081 cgcagccaca tgcccgccgg ggtgtcaagg aacctcagca ccctccggcc gcccagccgg
    10141 acggcggtcc ggcgcacgag ctcggggtgg ttgcggaccc aacggtacgc cagatagaga
    10201 gcgatagccg ctaccagcgc gacagccagc agcgcgctcg cccggccgat ccacttctcc
    10261 accaggccga cgctgccgcc caggaggtac ccgacgagca cggccgcagt cgcccaggtc
    10321 gcgccgccga gagcgttgta caggaagaag gtcctccagc gcatccggct caccccggcg
    10381 accagcgccc cgaagaccct cagacccgtg acgaagcgcg ccaggaagac cgccttgccg
    10441 ccgtgccggg cgaagaaggc ttcggcgcga gccaggcgtt ccggcgtgat caggacgtac
    10501 cggccccagc gcaggataaa cggacgccct cccctgcggc cgatccagta ccctatctgg
    10561 tccccgatca ccgctcccat gataccgaac aggatcgcgt cgccgagatc caacctgccc
    10621 tgctggacca ggatgcccgc ggagatgagg atggtctcgc cgggtagcgg gatccccatg
    10681 ctctcaagca tcacaccgaa gagcacgatc aggtaaccgt actgcgcgat gagggagagt
    10741 atgttctcga tggtgttcaa tgctcgccgc tatagtccag aggggcgccc gtaatactca
    10801 acctcttcat gctgtctagt ctatccttcc gttccatcca gtgatccgta tcctgcttcg
    10861 gacggtacaa agagttccta agaattcgtt aagaagctcc cggcgctccg gctctctcca
    10921 ctgccgcgac gacgagggtc aaaccggacc gggtcaggcc ggccgccgcc gactccaata
    10981 tcttgagccc cgcgcgcccg gtgagggcgg acacttcctt gaaggtcctc gggtagatcc
    11041 tgcggcggtt tgccagctcg gccgcggcgt agatgccgcc ttcgacgctg cgcagcggta
    11101 cggtaaagac gatccagcca ccaggcttca ggggcgcggc gagagagcgc aaggacgcgg
    11161 cgagatcctc cacgtagttg aggaccccga cggacaggac cccgtcgaac cccctcagcg
    11221 ctcccagatc gtccggcagc cttccgggcc gtatctccac gcccgtgacc ctctcgcgga
    11281 tcagccgctc tctcagatac cctcgcatct cgggcgagga gtctacggcc gtaatctccg
    11341 cgcagcggcg cgccagggga accgtatagt tcccggtccc gcatccaacc tccaggaccg
    11401 agtgccgggg ccgcatgagg ttgcccacga actcgaagat gacccgctcc tcccgatccc
    11461 tgaaagctgc gcccagaggg gtcccgaagt accgctcgta ggagcgcggg tcccatccgg
    11521 catcgttgga taggaaccac tctctaaata cccctctcac tcaacctcct ctctgcatcg
    11581 ttcagctccc gtccggctcc cggttcccgg aaccggtcag cgcgagggtc ccgaggatgg
    11641 ttagcgcgat gccgcctccg aggatgacgc gcgcaaagag ctccgtgggc caggactcgc
    11701 cgaagaggaa aggcgcgaag acgataggca cgacggtgtg gagggccagc actatcggga
    11761 ccacaaccga gacgcggccg tccctgaggg cgttaagctc ggtggcgaag ccccacagtc
    11821 cgagcgccac gatacagagc atgaggaggg cgagggacgg aatcttcgag acatggagat
    11881 cgtgggcgac gctcttgttg agtatcccgc tcatcgcgta ggccagcccc gccgccgtcg
    11941 ccgtcagggc ggcgccgggt gagcggcgga gtaatctcag ggcaccgggc gccagaatgc
    12001 cgggcgccag gagcgcgaag agcagaacgt actgcgggaa gcccggagaa gagttgccgg
    12061 tatcaggagg ggcgtagccg acggccacta tcccgagcgc cacgagtcca acgcccaggg
    12121 cctcgcggcg gccgaaggac tctttcaggg cccaacgggt catgccgagc aagaccacga
    12181 gcccggcggc gttcaggaca cgcgccaacg tcaggggtat cagggtaagc gcggcgacct
    12241 ccagcgccca tcccagcaag ttcatggaga tggcgaacgc accggaagcc tgccggccca
    12301 ccgccaggag tagggccgag tctcccgcgt ggcggcgcgt ctcggtggcg agcagcaccg
    12361 cggagccgtt ataggcgaac gtggaggcca ggatcagggc gacccctaga agcactcttc
    12421 ggaatccagc aggtctccca cggaacaggc tgcgagcccc atagagtcga gccgctccag
    12481 tatccggggc agcgcgccga gggtggcccg ccaggagccg ggagccgcgt acctgtcgga
    12541 gtcgtgcagc agcacgatat cgccgacggc tggaaggccg atctcatccg ccaccgagcg
    12601 aggcgtggcg cgggcctccc agtctcttcc ccagcgcgcc caaagtaccc gctcccaccc
    12661 ctgccggccg gcctcgatcc aggaggccgc gttgaagacg ccgtagggtg gccggaacag
    12721 gcaagtggga gcgccggtcg tctcctcgat caccgcccgc gcccggcgca tgtcctcgat
    12781 tgtctggcgc ggcgtcaggc gcaggtggtt gcgatgacgg taacagtgga ccgcgacctc
    12841 gtgcccgcgg gcgaaggtct ccttcagccg tccggggtcc cgctctacct gctccccgac
    12901 ggcgaagaac gttgcccgaa cttcgtactc ttccagcaag ccgaggaagg cttccaggca
    12961 agcgtccggc ccgtcgtcga aggtcagggc cacggctctc cggctcgcgc ggacctgcgt
    13021 gatcgcgggg aagaatagcc gtcccgtagt ggttagagcg ctcgcgggcg cggcgtgcgc
    13081 ggcgaggagg gcggagatca gagccggtcc ggcgatacgc attggagacc tttgtggtgt
    13141 acttgtcgca gagcgtcggc cagccgcgaa cgggccacct ccggggagag cccggaggag
    13201 tccacgacca gcactccggc gcggggtccg ccggcagtgt ctcgctcggc catgaccagc
    13261 cgggtgtgtg gcggcgcgtc cgccagcgcg gcgaggtccg tcttcggaga cggcaggaag
    13321 taccctgggt attcgccggt cgcgcgttgg agcgcggcgg cggcctcgcg gtcctcggac
    13381 ctgtctctca tgggcacggg tatccttctg cttccggcct cctccgcgac cccgaaggtg
    13441 agtcccggcg ccggatggag acccccggcc ccgcgcgcgc cggagaagat ggtcatgggc
    13501 gccctttcgt gttgggcgaa gtcctcgacg gcgcgggcgg tcttcgggtc gtccaccttg
    13561 acggcgagcg ccaccctgcc cggcgcggga tcgtagcccg gaacgtcgaa cctgaacgcc
    13621 ctggacgcca ccgctacccc cgaggaggcg aaggccgacc agaaaaagaa cgccgccgcg
    13681 gcggccccgg caagagcggg ccggaactgt ggaaccctcc ggcgcgccac cggacggggg
    13741 acgctcccgg atacggcttc gagcgcgata gcgatccccg gagcggaggt cttctcggga
    13801 gccggaagcc acatcgcgcc cgtggcggcg gatcgcagga gagcggtgag gtcctctccg
    13861 tcccgcgccc ggcggacgac gcccgcccgc tccatgaccc ggcagttgag ttctccgtga
    13921 ccccggatgg ggtcgaacat gactatgggc aggcccatct gcacagcctc caggcaggtc
    13981 atccccccgg cgttctgtat catgcagtcg gcggccgaca tgagagcggg caggtccttg
    14041 ctccacccca gtatccgcac gttctcttcg cgctcgaaac tcttctccag gcgctccttc
    14101 aggtccgagt tctcgcccgt caccacgacg gcgtacaccc cggcctcggc ggcgcgccgt
    14161 gcggcccctt cgaggtctcc gatcccccac gccccgccga ccacgagggc cacgaaggca
    14221 ctctgaggta tatcaagggc cgcgcgcgcc tcgtcatgtg gtagcgcaga gcggaaggct
    14281 ggtgccacgg gcatccggat cacggaggcg ctgccgccgg ctagttctgt aagctgcgcc
    14341 gacgggcccg agaccacgag atgaaggtcc aggcccggag ccacccacag aggatggacc
    14401 ccgtagtccg cgatcaccgc caccgccggt accttcagag caccgttcgc gcgcaggcgg
    14461 ccgagggcgg cgtttaccag cggatacgtc gagatcacca ggtccggccg ccgctcgttg
    14521 acaagatcta gcaaacggtg cgaaaagagc agccccacca gagagcgtat ggtccacgcg
    14581 cccatcgtcc aggaagtgac ggcgaagacg gccgccagag acctcggcgt gctcctgacc
    14641 tggttacagt agaacctggt gagcagccag tccaggccgc ggctcatcgc cttgagcccg
    14701 tccgcgacct ccacggtatg gcccgtccgc tccagctcgt cccgcagcgc ccggccagcg
    14761 gcctcgtgtc ccccgccgac agcagccgtg aggatgagga tcttctcggg atgagcgtcc
    14821 gggaggtagc tctcgcgggc ggtccgccgg aaccggacga ggcccgtcca caatagaaag
    14881 actgcgagcg cgactatcag gtagtcgatc atggcgatca gagctttccc acggttttat
    14941 cttctccgat tcttctgatt ggccgcgacc ccagattctc caggaaacca ttatctcatt
    15001 gaatactaag agagtggtaa gaacgggcaa gatgtagaaa actctatcaa actgcgctct
    15061 cggcttcggg gcggatgcgc cgccaattac cggggatctt cagcgtgaag atagagccct
    15121 tgccgggttg gctctcggcc tctatcgtgc cgccgtgggc ctcggcgatc tggcgggcta
    15181 tcgagaggcc gagaccggcg ccgcccgccg aagggccttg ccccgcccgg gccgcgtcca
    15241 cccgatgaaa acgctcgaag atcagcggca gttgctcctc cgggatgccg atgcccgtat
    15301 ccgagaccac aagctctgcc cagccgccgc gctctcgcgc cgccaccgtc accctgccgc
    15361 cgtccggcgt gtacttgatg gcgttatcga gcaaaatggc cgcgacctgc cggacgcggt
    15421 tgcggtcgaa cgtggcctcg accggaccat ccggctggtc atactccacc ttcagacggt
    15481 ccccaacgat ggtccgggcg gtctccgccg cctccgccgc gacggtcgcg aggtccgcgg
    15541 gctctacccg catcggcggt ccctcgtcgc cgcgggtgag ggccaggagg ctctcggcca
    15601 gcgctcgcag gcggtcggtc tcgttgcgga tggccgtgat actctcgcgg gcgaccgtcg
    15661 gatcttcgag gccccactcg tccagcatcc gggcgtagcc gctgatcgag gtcaggggcg
    15721 tgcgaagctc gtggctcgcg tcggcggcga accggcgctg gcggctgagt gcttcttcgc
    15781 ggcgggcgaa ggcggcttcg aggcgcgaga ggagcgcgtt gatggtaacc gcgaggcgcc
    15841 cgatctcatc cctcgggttg ctgaccggga gacgctttgc gaggtcgctc tccgtgatct
    15901 cccgcgccga acctacgacc gtctcgacgg gccgcagggc cgcgcgggcg agcaggtaag
    15961 cgccgaccag cgagaccagg aacgccgcac cgatgacgat gatgaggagc gcgtcgaaga
    16021 cctccagcgt gttctcgacc tcgcgggaag acttcccggc ctcgacgacc cgcgccgtcc
    16081 cggttttcgg cttgaccggg acggcgtaga catagtccgc gccttcttcg tgcgagatct
    16141 ccgccttccc atagacgggc ttcccggact ccagagcccg gcgccacacc cggtcgctta
    16201 tcttcccgtt gtcggggaag ttgtccgtga ggtccagtat ctggccccgc ttatcccgca
    16261 ccatgaggta gacctcgttc ggagcgagcc gcccggcctc gcccaggccg tcgcccgcct
    16321 cgacgctccg ggcggcggcg acggcctcgc cgcggaccgt ctcctcgatg ttggcgaaga
    16381 gagcgttgcg gagcagtaaa aatagcgcga gcccgaggaa gagcagtatc aagccgatag
    16441 cgagggcgtt gaagatcgtg agccgccacc ggatcggcac taccgctccc ttagagcgta
    16501 ccccgccccc cgcaccgtct ggatgaggcg cgactcgccc ggaagatcca ccttcttccg
    16561 caggtatccg acgtagacgt ctaccacgtt cgtcccgacc ccgaactcgt cgtcccacac
    16621 ccgcgagagc agcacctccc gcgagagcac ccggcggtgg ttgcgcgcca tgaactcaag
    16681 cagctcatat tcgcggtttg tcaggtctat ctcgcgctcg gtagagcctt caccgcgccg
    16741 gacctcgcgc gtagcggtgt ttatccgcag gtccgcgaca cgcagcacct cctcgccgtc
    16801 gta
//
LOCUS       DASSMB010000148         5734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_540406, whole genome shotgun sequence.
ACCESSION   DASSMB010000148 DASSMB010000000
VERSION     DASSMB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540406"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VFJ72_08795"
     CDS             <1..459
                     /locus_tag="VFJ72_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479595.1"
                     /translation="HAYHHLAYLEPDSGALFAGDVAGIRLPGQSYVRPPTPPPEIDVD
                     AWIKSINHIRRITPASLYPTHFGCYDDVERHLGELEQRLQDWLLFVEERMDGGAGSEE
                     IADELKDKGDAEMLAEGADTEETRHYDLAGNYEMLTIGIMRYVERRRKTA"
     gene            complement(471..1712)
                     /locus_tag="VFJ72_08800"
     CDS             complement(471..1712)
                     /locus_tag="VFJ72_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479596.1"
                     /translation="MDAGAREQGIRLGLRENLGQFLLLVIVNAFVGGMVGLERTVVPL
                     LGSQVFGLAFKTAIFSFIITFGLVKAFSNLLAGGLADRFGRKKILIAGWIVGVPAPFM
                     IMLAPSWGWIVAANVLLGVNQGLAWSMTVLMKIDLVGPRGRGLAVGLNEFAGYLAVGI
                     TAFLTGYLASTYGLRPEPFYLGAGYAVLGLLISVLLVRNTGGHVRLEMESHPPEPSPL
                     SFREVFARTSFKDRNLFACSQAGLVNNLNDGMSWGVFPLFFAAFGLGVERIGILKAVY
                     PAVWGTLQIATGPLSDRWGRKGLIVSGMWVQAAGIFVTVATRDFRWWLLGSVLLGLGT
                     AMVYPCLIAAVSDASNPSWRARSLSVYRFWRDLGYAIGALSAGIIADTLGLSWAIGSV
                     GMLTFLSGALFAVSFTEKRGK"
     gene            complement(1724..2806)
                     /locus_tag="VFJ72_08805"
     CDS             complement(1724..2806)
                     /locus_tag="VFJ72_08805"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7479597.1"
                     /translation="MALREMLERGDPIVVVDVRKVEEHAEWAIPGSVHYDAYDALNAG
                     DEHAMRGFDLPDGAPVVTVCGAGKTSLIAADQLRRRGCEASSLEGGMKAWSLAWNAAE
                     VETPGSDAKVVQIRRTGKGCLSYLIGSEGEAAVIDASVAPEVYSNLAGERGWRISHVF
                     DTHVHADHLSRSRMLAELTGARLYMPNGAPLSYAFSPLASGDTLEVGSARVEVLRTPG
                     HTGESTSYLLDGSALFTGDTLFLSSVGRPDLGATPARARDKARKLYDSLERLLKLGPD
                     TLVLPGHASEPVPFDGKPVAALLSEVRENVEILRESEETFAERVADRVPAAPSNYESI
                     VGLNRAGRFPEDLTDLEAGANRCAAG"
     gene            complement(2889..3026)
                     /locus_tag="VFJ72_08810"
     CDS             complement(2889..3026)
                     /locus_tag="VFJ72_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479598.1"
                     /translation="MAAGFEGFEITWRKDVFSGAPQAGSAANFGTLGINFRARKTAYE
                     E"
     gene            complement(3069..3686)
                     /locus_tag="VFJ72_08815"
     CDS             complement(3069..3686)
                     /locus_tag="VFJ72_08815"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7479599.1"
                     /translation="MSEQREVAQELDVGTLREAIQEEYAEVANHPTKGFHFHTGRPLA
                     RILEYRDEWLEGIPESSIESFAGTGNPFSLGVLQAGEQVVDVGSGAGIDSLIAARMVG
                     PEGRVVGVDMTQVMLEKARAAAAEADLENVEFHQSYAEELPVPDGWADVVISNGVLNL
                     MPDKAAVLGEMFRVLKPGGRLQVGDILVQRAVPEGARRNIDLWTG"
     gene            complement(3686..4369)
                     /locus_tag="VFJ72_08820"
     CDS             complement(3686..4369)
                     /locus_tag="VFJ72_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7479600.1"
                     /translation="MFKDQLFEQFARIGKALANPHRLELLDLLAQGERTVEDLAGEAG
                     MPVANTSRHLQELRAARLVEVRRDGLYGYYRLAGDGVFEVWRAIRDLGEARLAEIDRL
                     VGTYLTDRESLEAVCAEELLAKTREGDVVVLDVRPEEEYRAGHIPGARSVPLKKLEAY
                     LEEIPKDREVVAYCRGPYCVFSDEAVALLKSRGYRARRLAEGLPDWRAAGMPVEDDLT
                     NVSSEGKES"
     gene            complement(4663..4989)
                     /locus_tag="VFJ72_08825"
     CDS             complement(4663..4989)
                     /locus_tag="VFJ72_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479601.1"
                     /translation="MKHKRGLVVHRVGVESLLELPVKIQRQAVKKMRQVIEVAPESTG
                     YPLRGPLQGFRGIHSGRYRIIWRLLTLENGDQIPEVVYVGIRSEGDDRDAYREVEKVL
                     GTLEGF"
     gene            complement(4979..5293)
                     /locus_tag="VFJ72_08830"
     CDS             complement(4979..5293)
                     /locus_tag="VFJ72_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479602.1"
                     /translation="MIKLPEILGVAEARDSLSHILEGVGRGDTFIIRGPKGKEALVVN
                     ADSFRRMQEAYAELVGELETMKILQDEKAMEALRSVSDKDDAGERYSLTEVEGMVSED
                     ET"
     gene            complement(5596..5730)
                     /locus_tag="VFJ72_08835"
     CDS             complement(5596..5730)
                     /locus_tag="VFJ72_08835"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7479603.1"
                     /translation="MKRSLSPGFAGVDNPLFYETDKTMMLFSDAKQGLQEVVEAVKSG
                     "
BASE COUNT         1081 a         1917 c         1657 g         1079 t
ORIGIN      
        1 cacgcctacc accacctcgc ctacctggag ccggactccg gcgcgctctt cgccggagac
       61 gtggcgggca tacgcctgcc cggacagtcc tacgtccgcc cgccgacccc gccgccggag
      121 atcgacgtgg acgcctggat caagagcata aaccacatcc ggcggataac accggcgagc
      181 ctctatccca cgcacttcgg ttgctacgac gacgtggagc ggcacctcgg cgagctggag
      241 cagcgcctgc aagactggct cctcttcgtc gaagagcgga tggacggagg agccgggagc
      301 gaggagatcg ccgacgagct aaaagacaag ggcgacgccg agatgctcgc cgagggagcc
      361 gacaccgaag agacccggca ctacgacctc gccggcaact acgagatgct caccatcggg
      421 atcatgcgct atgttgagag acggcgaaag accgcctgac ggggccgaac ctacttccct
      481 cgcttctccg tgaacgacac tgcgaacagc gcgccggaga ggaacgtgag catccccacc
      541 gagcctatag cccaggagag tcccagcgta tcggcgatga tcccggcaga gagcgccccg
      601 atagcgtagc caaggtccct ccagaaccgg tagacgctca gggagcgcgc ccgccaggat
      661 ggattggagg cgtcggagac ggcggctatg agacaggggt agaccatcgc cgtgccgaga
      721 cccagcaaga cgctaccgag gagccaccac cggaagtccc gcgtcgccac ggtgacgaag
      781 atgcccgccg cctgcaccca catgccggag acgatcaggc ccttccggcc ccagcggtcg
      841 ctgagtgggc cagtcgcgat ctgcagcgtt ccccaaaccg ccgggtagac agccttgagg
      901 ataccgatcc tctccacccc gagcccgaag gcggcgaaga acagcgggaa gacgccccag
      961 ctcatgccgt cgttgaggtt gttgacgaga cccgcctgcg agcaggcgaa gaggttgcgg
     1021 tctttgaagg aagtcctggc gaatacctcc cggaagctca gagggctcgg ctcgggcggg
     1081 tgagactcca tctccagccg gacgtgcccg cccgtattcc ggacgaggag gactgagatc
     1141 aggagcccca ggacggcata gccggctccc agatagaacg gctcgggtct caggccatag
     1201 gtgctcgcca ggtagccggt caggaaagcg gttatgccga cggccaggta accggcgaac
     1261 tcgttgaggc ctaccgcgag accgcggccg cgcggtccca ccaggtctat cttcatcagg
     1321 accgtcatgg accaggctag gccctggttc acgccgagca gcacgttggc cgccacgatc
     1381 cacccccacg agggggcgag catgatcatg aacggcgccg gtacaccgac gatccagccc
     1441 gcaattagga tcttcttcct gccgaagcgg tcggcgagtc ccccggcaag gaggttggag
     1501 aacgccttga cgagcccgaa cgtgatgatg aacgagaaga tggcggtctt gaatgcaagc
     1561 ccgaagacct gcgagcccag gagcggcacc accgtgcgct ccagaccgac catcccgccc
     1621 acgaaagcgt tgacgatgac cagcaggagg aactgcccga ggttctccct gagcccgaga
     1681 cggatgcctt gctctcgcgc tccggcgtcc atgccggacc tcctcagccc gccgcgcagc
     1741 ggttggcgcc ggcctccagg tctgtgagat cttcggggaa ccggccggcc cggttgagac
     1801 cgacgatgct ctcgtagttc gagggcgcgg ccgggacacg gtccgcgacc ctctccgcga
     1861 aagtctcctc actctcccgc aggatctcga cgttctcgcg tacttcggag agtagcgcgg
     1921 cgaccggctt accgtcgaag gggaccggct cgctcgcgtg tccgggcagg acgagcgtat
     1981 cgggaccgag cttcagcaga cgttcgagcg agtcgtacag cttgcgcgcc ttgtcgcgcg
     2041 cccgcgctgg cgtcgcgccg aggtccggtc ttccgacgga ggagaggaag agcgtgtctc
     2101 cggtgaagag cgccgatcca tccagcaggt aactcgtgct ctctccggtg tgccccggcg
     2161 tccgaaggac ttcgactctc gccgatccca cctccagcgt gtcgccgctt gcgagcggcg
     2221 agaatgcgta ggagagcggc gctccgttgg gcatgtagag cctcgccccg gtaagctccg
     2281 ccagcatcct cgaacgagag aggtggtcgg cgtgcacgtg ggtgtcgaag acatgggaga
     2341 tccgccatcc gcgctccccg gcaaggtttg agtagacctc cggggccacc gaggcgtcga
     2401 tcacggccgc ttcgccctcc gagccgataa ggtaggagag acagcccttg ccagtacgtc
     2461 tgatctggac aaccttcgcg tcggagccgg gcgtctctac ctctgccgcg ttccatgcga
     2521 ggctccaggc cttcatgcca ccttccagcg aggaagcctc gcaacctctg cgccgtagct
     2581 gatcggcggc gatcaggctg gtcttgccag ccccgcaaac ggttaccacg ggcgcaccgt
     2641 ccgggaggtc gaaaccccgc atggcatgct cgtctccggc gttcagggca tcgtaggcgt
     2701 cgtagtggac gctgccgggg atcgcccact ccgcgtgttc ctccacttta cgtacgtcaa
     2761 cgaccacgat cggatctccc cgctccagca tctccctcaa ggccattgcg ctgagggtac
     2821 cgccccgcgg agatgcacct acgtcagcag gtcgaacaga cagccgacgg ttagatggca
     2881 cggcctcctc attcctcgta ggccgtcttg cgggcccgga agtttatgcc cagggtcccg
     2941 aagttcgccg cgctgcccgc ctggggcgcg ccgctgaaca cgtccttgcg ccaggtgatc
     3001 tcaaaccctt cgaaaccggc ggccacgacc gtggcctcta gctctgcctc cagcagagca
     3061 ccggcgattc acccggtcca gaggtctata ttgcgtctgg cgccctcggg cacggccctc
     3121 tgcaccagga tgtccccgac ctgtaaccgc ccgcccggct tgaggacgcg gaacatctcc
     3181 cccagcacgg cggccttgtc cggcatcagg tttagcacgc cgttcgagat caccacgtcg
     3241 gcccagccgt ccgggacggg caactcctct gcgtagctct gatggaactc cacgttctcc
     3301 agatcagcct ccgcagccgc ggctcgcgcc ttttcgagca taacctgggt catgtcaacg
     3361 ccgactacac gcccttcggg accgaccatc ctggcggcga tcaggctgtc tatcccggcg
     3421 cctgagccga cgtccaccac ctgctcaccg gcttgcaaca cgcccaggct gaacgggtta
     3481 cccgtaccgg cgaacgactc tatcgatgac tccgggatgc cctccagcca ctcgtcccga
     3541 tattccagta tgcgcgccag cggacggccc gtgtggaagt ggaagccctt tgtcgggtgg
     3601 ttcgcgacct cggcgtactc ctcctgtatc gcctcccgca aggtccctac atctagctct
     3661 tgcgctacct ctcgctgctc gctcactacg actcctttcc ttcgctactc acgttcgtaa
     3721 gatcgtcctc gaccggcatg cccgccgccc gccagtccgg tagtccttcg gccagcctcc
     3781 tggcccgata accgcgcgac ttgagcagcg ccaccgcctc gtcggagaac acgcagtacg
     3841 gcccgcggca gtaggcgacg acctcccggt ctttcgggat ctcctccaga taagcctcca
     3901 gctttttgag tggtacggaa cgcgcgccgg ggatgtgccc cgcccggtac tcctcctccg
     3961 ggcgcacgtc cagcactacc acatcgccct cccgcgtctt cgccagcaac tcctccgcgc
     4021 agacggcttc cagcgactcc cggtccgtga ggtaggtccc gaccagacgg tctatctccg
     4081 cgagcctcgc ttcccccaga tcccgtatcg cacgccacac ctcgaacacg ccatcaccgg
     4141 ccagccggta gtatccgtac agcccatccc gcctcacctc aacaagcctc gctgcccgga
     4201 gctcctgcaa atgccgggag gtgttcgcca ccggcatgcc cgcctcgccc gccaggtcct
     4261 caaccgtacg ctccccctga gccagcaagt ccagaagctc cagccggtgc gggttcgcca
     4321 gcgccttccc gatcctggcg aactgctcga ataactgatc cttgaacacc ctatggtcat
     4381 tcatgtattc aatataatta ttgaatacga gaaaagcaag agttgctgca aagatttcgg
     4441 ctatatcttt tggacggttg ggttaggcta gctccctcct gcaagaattg aaagcaagta
     4501 tagaattacc aatgaactaa agagcgagcg caacgccgag atgctcgccg agggcgcgga
     4561 tataagagag tccgagcact acgacttcgc cggcagctaa gagatgctga ccgtcgggat
     4621 catgcggtat gtggagcggc ggcgcaaaga tgctcccgca ctctaaaatc cctctaaggt
     4681 acctagcacc ttctctactt ctcggtatgc gtcacggtcg tcaccttccg agcggatgcc
     4741 cacatacact acttcgggga tttgatctcc gttctccagc gtaagtagac gccagatgat
     4801 ccggtacctg ccgctgtgta tgcctctgaa accttgaagc ggaccacgaa gaggatagcc
     4861 tgtgctctcc ggggcgacct ctataacctg acgcatcttc ttcacggctt gacgctggat
     4921 tttgaccggc aactctagaa ggctctctac gccaacccgg tggactacaa gtccacgttt
     4981 atgtttcatc ctccgatacc ataccttcca cctcggtcag cgagtatcgt tcaccggcgt
     5041 cgtccttatc cgacacagac cgaagtgctt ccatagcttt ctcgtcctgg agtatcttca
     5101 tggtctccag ttcaccgacc agttccgcgt acgcttcctg catcctgcgg aacgaatcag
     5161 cgttcacgac gagcgcctct ttgccctttg gccctcttat gatgaacgta tcgcctcggc
     5221 ctactccttc gagtatgtgg gatagtgagt ccctggcttc cgccactcca aggatctccg
     5281 ggagctttat cattgcgcct ttactcctgt aagttatggt tgcgttggtt tgacgtttgc
     5341 ttgtgcgctg tgcccgcgtt cgtactgtgt tggcaattta gttctaactg actctatgcc
     5401 ggattagcta ttttgggcgt ctgctttttg ctgtatgtct tttcgcgtgt attgtggtga
     5461 gcggcgcttt tgctctcaat actattgttt gcttgtcgta ctatgtctac aaagtcacct
     5521 cctcgggacg atctacgatg ttaatgtaca aaattatgta caaaaaacaa gaaaattagt
     5581 acgtcagatt gagagctagc cgctcttgac ggcctcaacg acctcttgaa gaccctgttt
     5641 ggcgtcggag aagagcatca tggtcttgtc ggtctcgtag aagagggggt tgtctactcc
     5701 ggcgaagccg gggctgaggg agcgtttgat gaag
//
LOCUS       DASSMB010000149        18921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_153551, whole genome shotgun sequence.
ACCESSION   DASSMB010000149 DASSMB010000000
VERSION     DASSMB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 18921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18921
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153551"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="VFJ72_08840"
     CDS             complement(<1..1866)
                     /locus_tag="VFJ72_08840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HET7479604.1"
                     /translation="MQNDNLVRMSRRRRRERNRRNLWGRVRTMFLFFCAACVLAMVGL
                     FASFSYTYATVADDLPNLDNYSSTELAQTSVVYDAKGNVISELYGVQNRYVVGLKDIN
                     PNLQKAVVAIEDHRFYEHRGVDFEAIARAAQRNIETMSIQEGGSTLTQQLIKNTYIAQ
                     KERSVPSFQRKIDEAALAWQYEKEHSKKEILEQYLNTVYFGANAYGAEAAAKTYFNKS
                     AKDLDLAESAMLAGIINLPGTYDPFTDPKSTEERRNVVLDNMLKYGDISKKEHDKAVK
                     QPLELSRGRVQAQDKNEYFLDAVRKELAREYGDDMVYEGGLKIRTTLDPDLQRMASEA
                     VDQVVDPEAGDPSASLVSVDPGTGAVKAMVGGSDFDKVKFNLATQAQRQPGSSFKSFV
                     LAEAINQGVSPKTVYDSHDLKIPLPAGSDKPYYEVANYGNIQRGPITIEEATEQSDNT
                     VFVQLALDLGMKKVVEMAHKMGITSPLDPYPATAIGGLRVGVNPLEMASAYSTFANAG
                     LHMKPYLIQKVTREKDGQEEVVEEHRLEGDRALSRDEAAAAVEVLRGVVERGTASQFH
                     NLDAELGRPSAGKTGTTEEFADAWYVGFIPQLSTSVWVGYPGERRSMVNINGLTEI"
     gene            1994..3073
                     /locus_tag="VFJ72_08845"
     CDS             1994..3073
                     /locus_tag="VFJ72_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7479605.1"
                     /translation="MNSGGSGPPTSAVMEEMRAAEDLCAGSVYLQGVKLFYRQAEANA
                     RARNAAARLVGAAPEDIALTQNTTHGMNLGVASIDWREGDEVLSTTTEHPGCLVPLHA
                     LRRRFGVRVNLVSPPVSAEKVIEAITSRTRLVALSHVDWTTGEVFPLEEICAAARERG
                     VLTLIDGAQSVGNIQVDAPATGADMYAFTGHKWVLGPEGMGALYVRPDCDLPSPNTGY
                     ASLAEGAVFDLSGGYKLRSSARRFEASTMSPILAAGFAAAAEAAHERGAEGFEGIRNR
                     AELLMDLLSDLSRITLRSPRPAQTGLVSFEVEGIEPKDAVERLLEQRFVLRFIPAPNS
                     CVRASTHLFNTERELEALARAVGEL"
     gene            complement(3076..3315)
                     /locus_tag="VFJ72_08850"
     CDS             complement(3076..3315)
                     /locus_tag="VFJ72_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479606.1"
                     /translation="MSAGAEEVRPGDRIRKFDDGDILMPEEGSDARKTVRLEARRSGS
                     RARIAAKMPAGEYALTVFVRLPEGDATYGFHVIVE"
     gene            complement(3754..4410)
                     /locus_tag="VFJ72_08855"
     CDS             complement(3754..4410)
                     /locus_tag="VFJ72_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7479607.1"
                     /translation="MLNAALFDFDGTLVDTTEMIYQGMLHATNEVLGREIPRETLMAG
                     VGQPLPRQMELLDAEKAELLLESYRRHHEENHDALIGEFPGVPEALARLRATGVKIGV
                     VTSKRRASVEMAVRRFPFFEEVVDHFVSMEETTEHKPHPAPLLKGLEDLGGVPKEEAV
                     YVGDSPFDVQAAKAAGMTSVAVSWGAFSEDTLRAAEPDHLVPDIDAVVDLLLEMRSAG
                     "
     gene            complement(4411..5232)
                     /gene="surE"
                     /locus_tag="VFJ72_08860"
     CDS             complement(4411..5232)
                     /gene="surE"
                     /locus_tag="VFJ72_08860"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET7479608.1"
                     /translation="MRIVLTNDDGIDAPGLLAARQALEEVGEVLTVAPDRNRSGVGRS
                     ISFGRSIRVEERKMADGAVGYACSGTPVDCVRLVALGLMDFEPDIVVAGINHGENLGD
                     DITYSGTVAGALEGIVIGVPGIAVSLSIDRPWHESAQEDFDMNGRLHFEPVARFTARL
                     VEIALKSLPDGRILSVNAPNVPSEKLRGARITRLGRRFYKDELIEVRDEEGRVGYDIY
                     NNPPGFHQEEGTDFAAISNREISITPVHLELTDEPGLKELGTWDVGSLLSENGHR"
     gene            complement(5324..7111)
                     /locus_tag="VFJ72_08865"
     CDS             complement(5324..7111)
                     /locus_tag="VFJ72_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7479609.1"
                     /translation="MVHDGQAAARRKVGATYLAETTVARRSGVKVLLVEDNPGDARLV
                     SILLSEVSPNTFDISHAGRLGEAFEHLERSEFDVVLLDLSLPDSSGLEAVDAMQGVAP
                     YLPLVVLSGMDDEQMALEAIQSGAEDYLVKGRGDGDLISRAIRYAIQRKKAEERLAYL
                     SQYDSLTGLANRALFQDRLTQAVARAEREGEMVALLSLDLDRFKDFNDALGHGFGDML
                     LKTAANRLQGCVREGDTVARLGSDEFGIVLEDVPDVQFVASVAQRISAELTEPFTVHG
                     QEMFLSAGMGIAVRPPSEADALIRDASAALQRAKAAGPDNYQFFSPEMNVEAFERLAF
                     ESSLRRALERGEFELHYQPQVDLSTGRIVGAEALLRWNHPELGLISPAKFIPVLEETG
                     MILEIGAWVIQTACSQSRAWRDDGLPGIRVAVNLSAKQFEQQDLSAVVSGALEETGLS
                     PDDLELEITESLLMQDSEESNAKLGRIKSERGVRVSIDDFGTGYSSLAYLKRFPIDAL
                     KVDQSFIRDLTEGSDDAAIVAAIIGLAHSLRLAVIAEGVETAAQLEYLRRQGCDEAQG
                     YYLSRPVPAAGFATLLRDAGTFLDSSEAS"
     gene            complement(7072..7530)
                     /locus_tag="VFJ72_08870"
     CDS             complement(7072..7530)
                     /locus_tag="VFJ72_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7479610.1"
                     /translation="MSKIPNAQPIEILLVEDNPGDARLAVEAFKDGAVRFATNLHHVK
                     DGSEALDFLYRRGGHSSAVRPDLVLLDLNLPRKDGREVLAEVKGDDDLKLIPILILTT
                     SQAERDILDTYGLHANGYITKPIDLDEFASVVESIKDFWFTTAKLPQEER"
     gene            complement(7527..11465)
                     /locus_tag="VFJ72_08875"
     CDS             complement(7527..11465)
                     /locus_tag="VFJ72_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479611.1"
                     /translation="MKRPVWLRYGFAVLVVAATVLLKLAIAVTIEGETPFLLLFFAVL
                     VSAWRGGVGPGLLATGLATLAADYFFIAPTYSFGFGNIGQAARLFQFILEGTFTTFII
                     AALQTSSRRAEERRTEIAAYQEDLEESEERYRLVVEGSNDGVWDWDVRNGGVYWNDRL
                     FEILGLSRSETEPSFDLFVDLIHPDDRKRTIDALTAHLERGETYEAEFRWRHASGEYR
                     TCLGRGKAQRDASGAPVRMAGIVQDITERKQRENSQRFMSEASAVLASSLDYQKTLAS
                     VARLAVPVLADWCAVDVVEEDGTVKRLAVAHEDPEKVALAHELQERYPQDPQAGYGVS
                     RVLRTGEPELVPEIPEGLLFSAIRDEDHRALLRRLNLKSYMVVPLLARGRTLGTITMV
                     MSDSGRRYSEDDLEVAEDLARRAAVSVDNARLYMEARREISDREKVERVLRQSEAMHR
                     SVVDSAFDAVLTMGADGIVRSFNKGAERVFGYASEEVVGQPMTMLMPERFREAHNAGL
                     RRYLETGEARIVGKDVLELPGLRKGGEEVSVELSITEVSGSEHPLFTGIARDVTERQR
                     TEQALRQSEERYRAVIEQAAEGIVLVDADDHHILEANPEFLRMFGYTSEEAAGLTIYD
                     LVADDRETVDRNIEITLEEGNRVVGERRYRCKDGSFLDVIVSGSAISYDGHRAIGIVI
                     RDITERKKAEERIRQQADLLDLASEPIFAWELGSGIVYWNRGCEDLYGFSTAEALGQR
                     SHRLLRTVHPVPREQFESLLEREGRWFGEVTHTTRDGRRLTVESRQMLTRTSDGRRLV
                     LETNRDITESKKAEEELRRSERSLADAQRMARMGNFEYDFQRDRARWSDELYRIFGWW
                     AGEVAPRYKTFINAAHPEDRDLLRDAIREALRGGEGDTIEYRIVRPDGEVRYVETQYE
                     VRREGARPVELVGTVQDVTESRETERRLREAEARFRTLVEQIPAITYIQETPEAEVPK
                     SVTYMSPQYEDIFGYSPDAEMFDPDHWVKILHPDDRERVLAEDERTDETGDPFKVEYR
                     VYAKDGSIVWVRDEAVLVRDDQGRPLFWQGVQYDITDQKRIEQELEARAEELARSNAE
                     LERFAYIASHDLQEPLRMVSSYTQLLARRYKGRLDEDADEFIEYAVDGATRMQVLIND
                     LLTYSRVGTKGKEFAPLDLAEIFEAARDNLQIAIEESGASVTSEPLPQVMGDSTQLVQ
                     LFQNLIGNAIKFRGEEPPEVHVGAERRGREWVISVRDNGIGIDPQYAERVFVIFQRLH
                     TREDYAGTGIGLAVCKRIVERHGGRIWLESATGEGSTFFFTLPARESNRR"
     gene            11837..12856
                     /locus_tag="VFJ72_08880"
     CDS             11837..12856
                     /locus_tag="VFJ72_08880"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: protein motif:HMM:NF010496.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET7479612.1"
                     /translation="MRIDERAIRPIDAAEWFRHRSYDHATFLNVEELGRRKREQNLTV
                     SLVLPCRNVADTVGGIIDEVLAVNDRAPMGAPLVDQILAVDADSEDGTMEVASRHGAE
                     VYSENELLDHYGGAHGKGDAMWRSLSVARGDLIMYADADTRDFEPQFVYGTLGPIICV
                     PQVRFVKGAFRRPFKSSETVEQDGGGRVTELTTKPLFNLFYPELTGFVQPLAGEFVGD
                     KELFSSIPFLTGYAVETGIMIDVLRKVGLGAMAQVDLGTRQNRHQPLRDLSRMSYSVV
                     RAVARRMRQDGRLNQSRDPSLPEQLFQFSDYLHAVATPEGLKLQEYVEELVERPPMAE
                     VLRIG"
     gene            12867..13172
                     /locus_tag="VFJ72_08885"
     CDS             12867..13172
                     /locus_tag="VFJ72_08885"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479613.1"
                     /translation="MPGVHDLGGSPGGPIEKGQHEVEDWERLADAITIALDKKGVKTT
                     DQHRRAQEGLDPALYKSLSYYERWVAATEILLVEKGILSREEIDERAAEISHRWDGS"
     gene            13169..13468
                     /locus_tag="VFJ72_08890"
     CDS             13169..13468
                     /locus_tag="VFJ72_08890"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HET7479614.1"
                     /translation="MTAAFEPGDRVRVRASEKPGHVRTPSYLRGRTGQVEAVLGEFRN
                     PEDLAYGLSGAPERPLYKVSFRQADLWRGYEGPAEDHLYADVYEHWLESVEGENG"
     gene            13465..14091
                     /gene="nthA"
                     /locus_tag="VFJ72_08895"
     CDS             13465..14091
                     /gene="nthA"
                     /locus_tag="VFJ72_08895"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HET7479615.1"
                     /translation="MSSAHDHPHEEVRQGSSAYAARIRAIESLLLEKSVLTEAEIQDN
                     ISYMDSRSPANGARLVARAWVDPEFKARLLSDTKSAAMELGIDASGPVEFVVVENTPQ
                     VHNLIVCTLCSCYPRAILGRPPDWYKSFDYRSRAVSEPRAVMHEFGFEPPEGVRVDVH
                     DSTADIRYMVLPLRPDGTEDMSEEELAALVTRDSLVGVSAPLAPARAG"
     gene            complement(14131..16173)
                     /gene="fusA"
                     /locus_tag="VFJ72_08900"
     CDS             complement(14131..16173)
                     /gene="fusA"
                     /locus_tag="VFJ72_08900"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7479616.1"
                     /translation="MAVEPALIRNVCLIGHRGSGKTSLGEAMLGVASGRSGSQGGVLD
                     YSDEEVERGMTLGMSVACLEWKGRQVNVLDTPGDGGFVADAFVAQRVADCAVLVVHAQ
                     DPIQVVTERVWRRGEREEIPHMVVVNHLDRERTDFMGVVEQLRGRFGQSIVPLNLPIG
                     RENDLRGVYGLLSGIAFVDGKQSEEIPAGMEDEVDAAKVALFEAIAESDDTLLEKYLE
                     GEDLTTEETFEGIRKGIADGLIIPVLAASAERMIGVDRLLDVIAGSAPSPVDRSRWIS
                     EDGDEVPCDPDGPFAAYVFKTYVDPFAGRLSVLRVVSGRCRSDEALTNPRTGSSERLG
                     SVSHLVGKERRPVDEAVAGDIVAVAKLRDTHTFDTLCKPENPITFEPVELPEPTTAFA
                     VGAQTRGEEEKVFEAIKRVVDEDPSLKLERSESTGEDILAGLAQMHVEFALERIHRRY
                     GVEVEARAPKVPFMETIQGSAQAQGRYKKQTGGRGQFGDCHIELTPQPRGAGFEFENA
                     IVGGAIPRQYIPAVEKGILEAMREGSIAGCPVVDVKVRLYDGSFHSVDSSEMAFKVAG
                     SMAFKNAVEKANPVLLEPFVKVEVLAPAELVGDIMGDLSGRRGRPMGIEQRGERQVIQ
                     AEVPQVEMLTYARDLRSITGGRANFHVEPGHYEEVPPNMVEKVLAANEREEEKAVS"
     gene            16328..16678
                     /locus_tag="VFJ72_08905"
     CDS             16328..16678
                     /locus_tag="VFJ72_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479617.1"
                     /translation="MGTFAGNTGITRTWPVLVVLVSGLLTYFAPHDEAYAGADYGISL
                     LATLGAAGCIGYLASRISLRLDLLPRLRPAASGLLPSALTTAGPETGEDWPVLVALLF
                     GLFLMLLAGLGGWV"
     gene            16807..17643
                     /locus_tag="VFJ72_08910"
     CDS             16807..17643
                     /locus_tag="VFJ72_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET7479618.1"
                     /translation="MVDLLKTIFGPLTDLLSGTLQTFHGWGAPWWLSIIMLTVIVRTL
                     LFPLTFKQVKSMRKMQELKPDLDEIKAKHKNDPKKQQEETMKLYGERSVNPLGGCLPV
                     LVQMPIFITLYYTIKSFEHLESFRTGGLLWFPDLTVSDPYFILPVLYVLTMMASQEIT
                     IRNTAPEQRQLMRFLPLVFGGFLAFSGFPAGLFVYWVASNCITFTQNYVIYHRHPHTA
                     PAKNGSEDAKGAKDTTPKPESAPKPGNEKSPAQKTTAATQNAPRSARSRNRKKKSAGK
                     KR"
     gene            17755..18168
                     /locus_tag="VFJ72_08915"
     CDS             17755..18168
                     /locus_tag="VFJ72_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7479619.1"
                     /translation="MTTGQSAQTEQHPLHSDRGRTTIASSVVSSIVNVAAGEVDGLAM
                     SHGGTRLPGDNSPTVGEFFDRVSGGGSRTRGVSVEVGEREAAVDLTVNVVYGKPIAQV
                     TQAVRQNIINRVESLTGLDVTEVNITVNDVTFPQQ"
     gene            complement(18702..>18921)
                     /locus_tag="VFJ72_08920"
     CDS             complement(18702..>18921)
                     /locus_tag="VFJ72_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479620.1"
                     /translation="DGKVSPKTGLPNLLRLGVMMHAYRNEIILARPPRLVQTLLFGSL
                     ASIGRLLGYKAEYPYPHPRQAEAKPQTI"
BASE COUNT         3286 a         6505 c         5699 g         3431 t
ORIGIN      
        1 gatctccgta aggccgttta tgttgaccat cgagcgccgc tcgcccggat atcccaccca
       61 gacgcttgta gatagctgag ggataaagcc cacgtaccag gcatccgcga actcctcggt
      121 cgtgccggtc ttgcccgccg acggacggcc gagctctgca tcgaggttgt ggaactggct
      181 cgccgtaccc cgctcgacca cgccccggag cacctcgacg gccgccgccg cctcgtctct
      241 tgagagcgcc cgatccccct ccaggcggtg ctcctcgacg acctcctcct ggccgtcctt
      301 ttcgcgggtc accttctgga tcaggtacgg cttcatgtgg agtccggcgt tggcgaaggt
      361 cgagtacgcc gacgccatct ccagcgggtt gactcctacc cgcaggccac ctatcgcggt
      421 ggccgggtag gggtcgagcg ggctcgttat gcccatcttg tgcgccatct cgaccacctt
      481 cttcatgccc aggtcgagcg cgagttgcac gaagaccgtg ttatcggact gctcggtggc
      541 ctcttctata gtgatcgggc ctcgctgtat gttgccgtag ttggcgacct cgtagtacgg
      601 cttatcggat cccgccggca acggtatctt gaggtcgtgg gagtcgtaga cggtcttcgg
      661 ggagaccccc tggtttatgg cctccgcgag cacgaaggac ttgaacgagc tgccgggctg
      721 tctttgagcc tgggtggcta ggttgaactt gaccttgtcg aagtccgagc cgccgaccat
      781 cgccttcacc gcgcccgttc cggggtccac cgacaccagc gaagcggacg ggtcccccgc
      841 ctccgggtcc actacctgat ccacggcctc gctcgccatc ctctggaggt ccgggtccag
      901 ggtcgtgcgg atcttcaacc cgccctcgta gaccatgtcg tccccgtact ctctggcgag
      961 ctccttgcgg acggcgtcca ggaagtactc gttcttgtcc tgggcctgca cccgcccgcg
     1021 gctcaactcc aggggctgct tgaccgcctt gtcgtgctct ttcttgctga tatcgccgta
     1081 cttgagcatg ttgtcgagga cgacgttgcg ccgctcctcg gtgctcttgg ggtccgtgaa
     1141 cgggtcgtag gtgccgggga ggttgatgat tccggccagc atcgccgact ctgcgaggtc
     1201 gaggtctttg gcgctcttgt tgaagtaggt cttggccgcc gcctcggccc cgtaagcgtt
     1261 cgccccgaaa tagacggtgt tgaggtactg ctccaggatc tccttcttcg agtgctcttt
     1321 ctcgtactgc cacgctaacg cagcctcgtc gatcttgcgc tggaagctcg gcaccgagcg
     1381 ctctttctgc gcgatgtagg tgttcttgat gagctgctgg gtcagggtgg agccgccctc
     1441 ctgaatggac atggtctcga tgttcctctg ggccgccctg gctatggcct cgaaatccac
     1501 accccggtgc tcgtagaagc ggtggtcctc aatggcgacc acagccttct ggaggttggg
     1561 gttgatgtcc ttcaggccaa cgacgtagcg gttctgaacg ccgtacagct cactgatcac
     1621 gttccccttg gcatcgtaga ccacggaggt ctgggcaagc tcggttgacg agtagttgtc
     1681 caggttcggc aggtcatcgg cgaccgtggc gtaggtgtag ctgaagctcg cgaacagccc
     1741 caccatcgca agcacgcacg cagcgcagaa gaacagaaac atcgtgcgta cccggcccca
     1801 taggttgcgc ctgttccgct cgcgccgccg ccggcggctc atcctcacca aattatcgtt
     1861 ctgcataagc acgctattat aaccagatgc cacacaatat gagcaatgca tttctgaaag
     1921 gagccacaac atcggccatc caggactcgc ccgggacctc ttcccggcgc tcgccggcaa
     1981 cgactacgtc tacctgaact ccggcggcag cggaccgccg acctccgccg tgatggaaga
     2041 gatgcgcgcc gccgaagacc tctgcgcggg ctcggtctac ctccagggcg tgaagctctt
     2101 ctaccgccag gccgaggcga acgcgcgggc ccgcaacgcc gccgcccgcc tcgtgggagc
     2161 ggctcccgaa gacatcgccc tcacccagaa cacgacccac ggcatgaacc tcggcgtcgc
     2221 gtccatagac tggcgggagg gagacgaagt cctctcaacc accacggagc atccaggatg
     2281 cctggtcccg ctccacgcgc tgcgcaggcg cttcggagtc cgggtgaacc tcgtctcgcc
     2341 tccggtgagc gcggagaaag tgatcgaagc tataacatct cgcacgcggc tcgtcgccct
     2401 ctcgcacgtg gactggacga ccggagaggt gtttccgctg gaggagatct gcgccgccgc
     2461 tcgcgagcgc ggcgtcctga cccttataga cggcgcgcag tcggtcggca acatccaggt
     2521 ggacgctccg gcgaccggcg cggacatgta cgccttcacc ggccacaagt gggtcctcgg
     2581 ccccgagggg atgggcgctc tctacgtcag gcccgattgc gacctgccga gcccgaacac
     2641 cggatacgcc tcgctcgccg agggagccgt cttcgacctc tccggcggct acaagttgcg
     2701 gagcagcgcc cggcggttcg aggcgtccac catgagcccc atcctcgccg ccggtttcgc
     2761 cgccgccgcc gaagccgccc acgagcgcgg cgccgagggc ttcgagggga tacggaaccg
     2821 cgccgaactg ctaatggacc tgctctcaga cctttcccgc atcacgctcc gctcaccgcg
     2881 tcccgcgcag accgggctcg tcagcttcga ggtagagggg atcgaaccga aggacgccgt
     2941 cgagcggctc ctggaacaga ggttcgtgct ccgcttcatc ccggcaccga actcctgcgt
     3001 ccgggccagc acgcacctgt tcaacaccga aagagagctg gaggctctgg ccagagcagt
     3061 gggcgaactg tagagctact ccacgatgac gtggaacccg taggtagcgt cgccctccgg
     3121 cagccgcacg aataccgtca atgcgtactc cccggcaggc atcttcgcgg ctatgcgcgc
     3181 ccggctcccg gagcgccggg cctccaggcg tacggtcttg cgcgcgtcgc ttccctcctc
     3241 gggcatcagg atatctccgt catcgaactt cctgatgcga tcaccgggcc gcacctcctc
     3301 cgcccccgcg ctcatcgagt cgagcttcgc gcctccatac tcgaatgtca aaacagagcc
     3361 agcaggcacg gtcaacgccc gatcgctgat cggtattccg atcttgtccg cgcatacgga
     3421 tctcaaacag taagatccaa gaccagccct cacggtttcc ccttcgtaag agagcgtgct
     3481 cgcaggcggc tccggtagcc taccgggtcc ggctccctcc aacggaccgg aacccttccc
     3541 cgtcgtctcc gaaacggtct gcataggttt acggtcgtcg gttttctcgg caccgggagt
     3601 cgcagcctga ccgcaaccgc ccagaactat tccggcgagc attatgaaca gcggtataca
     3661 aaaaatacgt ctcgtaacca cgataaccag attataacta ttggcgagac cgcagggttt
     3721 ggatctgcct gtacagcgaa cggcgctcag gcttcagccg gcggagcgca tctccagcag
     3781 gaggtcaacc accgcgtcta tgtccgggac gaggtggtcg ggttcggcgg cgcgcagggt
     3841 gtcctcggag aaggcgcccc agctaacggc gacgctggtc attccggcgg cttttgcggc
     3901 ctgcacgtcg aacggggagt ctccgacgta gacggcctct tctttcggga cgccgccgag
     3961 gtcttcgagg cccttgagga gcggggccgg gtggggcttg tgctcggtgg tctcctccat
     4021 tgatacgaag tggtccacga cttcttcgaa gaatgggaac cggcggacgg ccatctcgac
     4081 ggaggcgcgg cgcttggagg tgacgacgcc gatcttcacg ccggtggcgc ggaggcgggc
     4141 tagagcctcg gggacgccgg ggaactctcc tatgagggcg tcgtggttct cttcgtggtg
     4201 gcgccggtag gattcgagga gcaactcggc cttctcggcg tcgaggagtt ccatctggcg
     4261 cgggagaggc tggccgacgc cggccatgag ggtctcgcgc ggtatctcgc gcccgagcac
     4321 ctcgttggtg gcgtggagca tgccctggta gatcatctcc gtcgtatcca cgagcgtgcc
     4381 gtcgaagtcg aagagggcgg cgtttagcat ttagcgatgt ccgttctcgg agagaaggct
     4441 gccgacgtcc caggtgccga gctccttcag gccgggttcg tcggtcagtt ccaggtgtac
     4501 gggggttatg ctgatctccc ggttcgatat cgccgcgaaa tccgtgccct cttcctggtg
     4561 gaagccgggc gggttgttgt agatgtcgta gccaacgcgc ccttcctcgt cgcgcacctc
     4621 gatcaactcg tccttgtaga accgacgccc gagacgggtg atcctcgctc cccggagctt
     4681 ctcggagggc acgttcgggg cgttgacgct gaggatgcgc ccatccggca ggctcttgag
     4741 cgcgatctcc acaagccggg cggtaaaacg cgccaccggc tcgaagtgca ggcggccgtt
     4801 catgtcgaaa tcctcctggg cgctctcgtg ccagggacgg tctatgctca aagagacggc
     4861 gatgccgggg accccgatca cgatcccctc cagcgcgccc gccaccgtgc cggagtacgt
     4921 tatgtcatca cccaggttct cgccgtggtt gatgcccgcc actacgatat ccggctcgaa
     4981 gtccatcagg cccagagcca cgagccgcac gcagtccacg ggcgtgccgc tgcaagcgta
     5041 gcccacggcc ccgtcggcca tcttccgctc ctcgacccgg atggagcgcc cgaacgagat
     5101 gctgcgcccg accccggatc ggttccggtc gggagcgacg gtgagcacct ccccgacctc
     5161 ctccagcgcc tgccgcgccg ccaggagacc cggagcgtct ataccatcat cgtttgtaag
     5221 gactattcgc atcttttccg gcttccgact agcgttcgct accactgctt ccaactcaag
     5281 ggtcccgatt ctaccatccg cggagagact ctccgtgaac ggtctagctc gcctcgctgg
     5341 aatcgagaaa tgttccggca tcccggagca gagttgcgaa cccggcggcc gggaccgggc
     5401 ggctaaggta gtagccctgt gcctcgtcgc agccctgccg ccgcaggtat tcgagttgag
     5461 ccgccgtctc gacgccctcg gcgatcaccg ccagccgcag gctgtgcgcg agcccgatga
     5521 tggccgcgac gatagccgcg tcgtccgagc cctccgtcag gtcccggata aaggactgat
     5581 ccaccttgag agcgtctatg gggaagcgtt tcaggtaggc gagcgaggag tacccggtgc
     5641 cgaagtcgtc tatcgagacg cgcacgccgc gctcggactt gatgcggccg agcttcgcgt
     5701 tgctctcctc gctgtcctgc atgagcaagc tctccgtgat ctccagctcc aggtcatccg
     5761 gggataatcc cgtctcttcc agcgccccgg agacgaccgc gctcaggtcc tgctgctcga
     5821 actgtttagc ggataggttc accgccaccc ggatccccgg cagtccgtca tctctccagg
     5881 cgcgggactg cgagcacgcg gtctgtatga cccaggcgcc gatctccaag atcatgccgg
     5941 tctcctccag cacggggatg aacttcgccg gggagatgag tcccaactcc gggtggttcc
     6001 agcgcaggag agcctcggct cccacgatgc gcccggtcga gaggtccacc tgcggctggt
     6061 agtgaagctc gaactccccg cgctccagcg cccggcgcag gctgctctcg aaggcgagcc
     6121 gctcgaaggc ttccacattc atctccggcg agaagaactg gtagttgtcc ggaccggccg
     6181 ccttggcccg ctggagcgcg gcgctggcat cccgaatcag agcatccgcc tccgacggtg
     6241 gacgcacggc gatgcccatg ccggccgaca ggaacatctc ctgcccgtgg acggtaaagg
     6301 gctcggttag ctccgcggag atcctctgcg ccaccgaggc cacgaactga acgtccggga
     6361 cgtcctccag gactatcccg aactcgtcgc tgccgagccg cgccacggta tccccctcgc
     6421 gcacacatcc ctgtagacgg ttcgcggcgg tcttgaggag catgtcgccg aatccatgcc
     6481 ctagcgcgtc gttgaagtcc ttgaaacggt ctagatcgag ggagagcagc gccaccatct
     6541 ctccttcgcg ctcggcgcgc gcgaccgcct gcgtgagccg gtcctggaac agggcgcggt
     6601 tggcgaggcc ggtcagagag tcgtattgag agaggtaggc cagccgttcc tcggccttct
     6661 tgcgctggat agcgtagcgg atcgcgcgcg agatcaaatc cccatctccc cggcccttga
     6721 ccaggtaatc ctccgcgcca ctctgaatgg cctcaagtgc catctgctcg tcgtccatac
     6781 cgctcaggac taccagcggc aggtagggcg ccacaccctg catggcgtca acggcttcga
     6841 ggccgctgga gtccggcagc gagaggtcga ggaggaccac gtcgaactcc gagcgctcca
     6901 ggtgctcgaa cgcctcgccc agacgcccgg cgtgcgagat gtcgaaggtg ttaggactca
     6961 cctccgagag caggatgctg accagccggg catcgccggg gttgtcctcg accagcagga
     7021 ccttgacgcc cgaccgccgc gctaccgttg tctccgcgag atacgtcgcg cctacctttc
     7081 ttcttgcggc agcttggccg tcgtgaacca gaagtctttt atcgactcca ccacgctggc
     7141 gaactcgtcc aggtctatgg gcttggtaat gtagccgttg gcatgcaggc cgtaagtatc
     7201 gaggatgtcg cgctcggcct gcgaggtggt caggatgagg atcgggatga gcttgaggtc
     7261 gtcatcgccc tttacctccg ccaacacctc gcggccgtcc ttgcgcggga ggttcaggtc
     7321 gagcagcacg aggtccgggc ggaccgccga ggaatgcccg cccctccggt acaggaagtc
     7381 cagagcctcg ctgccgtcct tcacgtggtg aaggttggtt gcaaatctga cggcgccgtc
     7441 cttgaacgcc tccaccgcca gccgggcgtc gcccgggttg tcttcgacca gcagtatctc
     7501 tatgggttgc gcgttcggga tcttactcac ctccggttgc tttctcttgc cggcagggtg
     7561 aagaagaacg tgctaccctc ccccgtcgcg gactcgagcc agattctacc gccatgacgc
     7621 tctactatgc gtttgcagac cgcgagaccg atcccggtcc cggcgtagtc ttccctggtg
     7681 tgcagccgct gaaagatcac gaagaccctc tccgcgtact gggggtctat cccgatacca
     7741 ttgtctcgca cggagatgac ccactccctg ccccgccgtt ccgccccgac gtggacctcc
     7801 ggcggctcct ccccccggaa cttgatggcg ttcccgatca ggttctgaaa gagctggact
     7861 agctgggtgg agtctcccat gacctgcggc agcggctccg aggtaacaga ggcgccgctc
     7921 tcctcgatgg cgatctgcag gttatcccgc gccgcctcga agatttcggc gaggtcaagc
     7981 ggggcgaact ccttaccctt cgtcccgacc cgcgagtagg tgagcaggtc gtttatgagg
     8041 acctgcatcc gggtggcgcc gtccaccgca tactctataa actcgtccgc gtcctcgtcg
     8101 agccggccct tgtaacgccg ggccaaaagc tgcgtgtaac tcgacaccat cctcaacggc
     8161 tcctgaaggt cgtgcgaggc gatgtacgcg aaacgctcca gttccgcgtt ggagcgggcg
     8221 agctcctcgg cacgcgcctc cagttcctgc tcgatccgtt tctggtccgt gatgtcgtac
     8281 tgaacgccct gccagaacag cggcctgccc tggtcatcgc gcaccagcac cgcctcgtcc
     8341 cggacccaga cgatgctgcc gtccttggcg tacacgcggt actcgacctt gaacgggtct
     8401 ccggtctcgt ccgtccgttc atcctcggcc agcacccgct cgcggtcgtc cgggtgaagt
     8461 atcttgaccc agtggtcggg gtcgaacatc tccgcgtccg gcgagtatcc gaagatgtcc
     8521 tcgtactgcg ggctcatata tgtcacgctc ttggggacct cggcttccgg ggtctcctgg
     8581 atgtaagtta tcgccggtat ctgctccacc agcgtgcgga accgggcctc cgcctcgcgg
     8641 agcctcctct cggtctcccg gctctcggtt acgtcctgaa cggtgccgac gagctccacg
     8701 ggccgcgcgc cctcgcgccg cacctcgtac tgcgtctcca cgtaccgaac ctcgccatcc
     8761 gggcgcacga tccggtactc gatagtatcg ccctccccgc cgcgcagggc ttcacggata
     8821 gcgtcccgca acagatcccg gtcctcgggg tgagccgcgt tgataaaggt cttgtagcgg
     8881 ggcgcgacct ctcccgccca ccacccgaag atccggtaga gctcgtcgga ccaccgcgcc
     8941 cggtcgcgct gaaagtcgta ctcgaagttt cccatgcgcg ccatccgctg cgcgtcggcc
     9001 aaactgcgct cgctgcgccg aagctcctcc tcggccttct tactctcggt aatgtctcgg
     9061 ttagtctcga gtacgagccg gcggccatcc gacgtgcggg tcaacatctg gcggctctcc
     9121 acggtaagcc tcctgccgtc gcgagtcgtg tgcgtcacct ctccgaacca ccgtccctcg
     9181 cgctccagga ggctttcgaa ctgctctctt ggtacgggat ggacggttcg gagcagtcgg
     9241 tggctccgtt gcccgagggc ctccgcggtc gaaaacccgt agagatcctc gcagccccgg
     9301 ttccagtaga cgataccgct cccaagctcc caggcgaaga tcggctccga cgccaggtcg
     9361 agcaggtccg cctgctgccg gatccgctcc tccgcctttt tgcgctcggt gatgtcgcgg
     9421 atgacgatgc cgatagccct gtgcccgtcg taagatatgg cgcttccgct tacgatgaca
     9481 tccaagaacg aaccgtcctt acaccggtag cggcgctcgc ccacgacgcg gttcccctct
     9541 tccagggtga tctcgatgtt gcggtccacg gtctcccgat catcggcaac caggtcgtag
     9601 atcgtaagtc ctgcggcctc ctcggaggtg tagccgaaca tccgcaggaa ctcggggttg
     9661 gcttccagga tgtgatggtc gtcggcatcg accaggacga tcccctccgc ggcctgctcg
     9721 atcacggccc ggtaacgctc ttcactctgg cgcagcgcct gttccgtacg ctgtcgttcg
     9781 gtgacgtcgc gagcgatacc ggtaaacagc gggtgttccg acccgctgac ctcggtgatg
     9841 gagagctcga ccgagacttc ctcgccgccc ttgcgcagtc ccggaagctc cagcacgtcc
     9901 ttccccacta tgcgcgcctc gccggtctcc aggtagcggc gcaaccccgc gttatgcgcc
     9961 tccctgaagc gctcgggcat cagcatggtc atcggctgtc ccacgacttc ctccgaggcg
    10021 tatccaaaga cgcgctccgc tcccttatta aacgaccgga cgataccgtc cgctcccatc
    10081 gttagcacgg cgtcgaaagc ggagtccacg acggaccggt gcatggcctc gctctggcgc
    10141 aacaccctct cgaccttctc ccggtcggag atctcccgcc gcgcctccat gtacagccgg
    10201 gcgttatcaa ccgacacggc cgcgcggcgg gccaggtcct cggccacctc cagatcatcc
    10261 tcgctgtagc gccgcccgga gtccgacatc accatcgtta tggtccccag ggtccgccca
    10321 cgggccagca acggcacgac catgtacgac ttgagattca ggcgtctcaa tagcgcccgg
    10381 tggtcctcat cccggatagc gctgaagagt aacccctcgg ggatctcggg caccagttcc
    10441 ggctccccgg tacgcagtac gcgcgacact ccgtacccgg cctgcgggtc ctgcggatac
    10501 cgctcctgaa gctcgtgcgc caaggcgacc ttctcggggt cctcgtgagc caccgccagc
    10561 cgcttgaccg tcccatcctc ctcgaccacg tccacggcgc accagtcggc gaggacgggc
    10621 accgccagcc gcgccacgct ggcgagcgtc ttctggtaat ccagcgagga agcgagcact
    10681 gcgctcgcct ccgacatgaa gcgctgcgag ttctccctct gcttacgctc cgtaatgtcc
    10741 tggacgatcc cggccattcg gaccggcgct ccgctcgcgt cgcgctgagc cttcccccgt
    10801 ccgagacagg tgcggtactc cccactcgca tggcgccacc ggaactcggc ctcgtacgtc
    10861 tcgccgcgct ccaggtgggc cgtcagagcg tctatcgttc tcttccggtc atccgggtgg
    10921 atcaggtcca cgaaaaggtc gaagctcggc tcggtctccg agcgcgagag cccgaggatc
    10981 tcgaagagcc tgtcgttcca gtagacgccg ccgttgcgga cgtcccagtc ccatacgcca
    11041 tcattggagc cctcgacaac cagtcggtag cgctcctcgc tctcctcgag atcctcctgg
    11101 taggcggcta tctccgtccg ccgctcctcg gcccgccggc tggaggtctg gagagcggcg
    11161 ataatgaaag tggtgaatgt cccctccagg atgaactgga acaaccgcgc ggcctgcccg
    11221 atattcccga agccgaagga gtacgtcggg gcgataaaga agtagtccgc ggccagcgtc
    11281 gccagcccgg tcgcgagcag gccgggtccc acgccgccgc gccaggcgct caccaggacg
    11341 gcgaagaata gcagcaggaa cggcgtctcg ccctcgatgg tcaccgcgat agccagcttg
    11401 agcaaaaccg ttgccgccac gaccagaacg gcgaacccgt agcgcaacca gacaggacgc
    11461 ttcacagcga cgatccttcc ttcatgccag aatcaccaga agccccgcga agcctccgaa
    11521 gacctctcgg gcggcggcgc atactaatcc ggcaattcgt catcacgttt cctgtccacc
    11581 ttacaacaag cagaagcaac acgttccgca tgttactctg atcgctactt ccatcgcaac
    11641 acaatataac aaggcgcgtg atcttgcgta gtttaccgga acatcccgca caatataccc
    11701 gtcgcatgca aggtcttggc cgagcttccc cgatctagag cgcttacatg tttccagcat
    11761 gttaaatttc gtttcgtata agccatgttc gcgtcccggt atagacatgg acgcatcgct
    11821 gggttaattt atgtgtatga gaatcgacga gagggctatt agacccatag acgccgcaga
    11881 gtggtttaga catcgttcct acgaccatgc aacgttcttg aatgtagaag agttgggccg
    11941 ccgcaagcgc gagcagaacc tgacggtgag cttagtgctg ccgtgccgca acgtcgcgga
    12001 cacggttggc ggaataatcg acgaagttct cgccgtaaac gaccgcgcgc cgatgggagc
    12061 gccgctggta gatcagatcc tggccgtgga cgccgactcg gaggacggca cgatggaggt
    12121 ggccagccgc cacggcgcgg aggtgtactc ggagaacgag ttgctcgatc actacggcgg
    12181 cgcgcacggc aagggcgacg ccatgtggcg ctctctctcc gtcgcccgcg gagacctgat
    12241 catgtacgcc gacgcggata cccgcgattt cgagccgcag ttcgtctacg gcactctagg
    12301 ccctatcatc tgcgtgccgc aggtccggtt cgtcaagggc gctttccggc gtcccttcaa
    12361 gagcagcgag accgtcgagc aggatggcgg gggccgcgtc acagagctca ccaccaagcc
    12421 gctcttcaac ctcttctacc cggagcttac cggcttcgtg caaccgctcg ccggcgagtt
    12481 tgtcggggac aaggagttgt tctcctccat ccccttcctg accggctacg cggtggagac
    12541 ggggatcatg atcgacgtgc tgcgcaaggt cggcctcggc gcgatggccc aggtggacct
    12601 cggcacgcgg cagaaccgcc accagcccct gcgcgacctc tccaggatgt cgtactctgt
    12661 cgtgcgggcg gtcgcgcggc gcatgcgcca ggacgggcgg ctcaaccagt ccagggaccc
    12721 gagcctgccc gagcaactct tccagttctc cgactacctg catgccgtcg cgaccccgga
    12781 ggggctcaag ctccaggagt acgtcgagga gcttgtcgag cgtccgccga tggccgaagt
    12841 actgcggatc gggtaggcgg ctcgctatgc cgggtgtcca cgacttaggc ggctcgcccg
    12901 gcggtccgat agagaagggt cagcacgagg tagaggactg ggagcgtctg gccgacgcca
    12961 tcaccatcgc cctggacaag aagggcgtaa agaccaccga ccagcaccgc cgcgcccagg
    13021 aggggctgga cccggcgctc tacaagagcc tgagctacta cgagcgctgg gtggcggcga
    13081 ccgagatcct gctggtcgag aagggcatac tcagccgcga agagatagac gagcgggcgg
    13141 cggagatctc ccaccgctgg gacggctcgt gacggccgcc ttcgagcccg gcgaccgggt
    13201 gagggtgcgc gcctcggaga agcccggcca cgtgcgcact ccttcctatc taagaggaag
    13261 gacgggacag gtcgaagccg tcctcggcga gttccgcaac ccggaggatc tggcatacgg
    13321 cctctccggc gcgcccgaga gacccctgta caaggtgagc tttcggcagg cggacctctg
    13381 gagaggttac gagggtcccg ccgaagacca cctctacgcc gatgtatacg agcactggct
    13441 ggagagcgtc gaaggagaga acggatgagc agcgcacacg accacccgca cgaggaagtg
    13501 cggcaaggga gcagcgccta cgccgcgcgc atcagggcga tagagtcgct cttgctggag
    13561 aagagcgtcc tgacggaggc ggagatacag gacaacatct cctacatgga ctcccgctct
    13621 cccgccaacg gcgcgcggct cgtggcccgc gcgtgggtgg acccggagtt caaggcgcgc
    13681 ctcctctccg acaccaagtc cgccgcgatg gagcttggga tagacgcttc cggccccgtc
    13741 gagttcgtcg tcgtggagaa cacgccgcag gtccacaacc tgatcgtctg cactttatgc
    13801 tcctgctacc cgcgcgccat cctcggccgt ccgccggact ggtacaagtc cttcgactac
    13861 cgctcccgcg ccgtgagcga gccccgcgcc gtcatgcacg agttcggctt cgagccgccg
    13921 gagggcgtcc gcgtggacgt ccacgacagc accgccgaca tccgctacat ggtcctcccg
    13981 ttgcggcccg atggaacaga ggacatgagc gaggaggagc tcgcggcact cgtaacccgc
    14041 gactccctgg tcggagtaag cgcgccgctc gctccggcgc gggcggggta acttcccgag
    14101 aaccgtcgcg ctgccgcgag ttcttttttc ctagctgacc gctttctctt cctcgcgctc
    14161 gttggcggcg agaactttct ctaccatgtt tggcgggact tcctcgtagt ggccgggctc
    14221 gacgtggaag ttcgcgcgac cgccggttat ggagcgcagg tcccgggcgt aggtgagcat
    14281 ctcgacctgt ggcacttcgg cctggatgac ctgccgctcc ccgcgctgct ctatgcccat
    14341 cggacgtccg cgccgccccg aaaggtcgcc catgatgtcg ccgaccagct ccgccggagc
    14401 gagcacctcg accttgacga acggctccag gagtacgggg ttcgccttct cgacggcgtt
    14461 cttgaaggcc atcgagccgg cgaccttgaa ggccatctcg gaggagtcaa ccgagtggaa
    14521 ggagccgtcg tagagcctca ccttcacgtc cacgaccggg cagccggcga tggagccctc
    14581 gcgcatcgct tccaggatgc ccttctcgac ggcggggatg tactggcggg ggatcgcccc
    14641 tccgactatc gcgttctcga actcgaagcc cgcgccgcgc ggctggggcg ttagctcgat
    14701 gtggcagtcg ccgaactgac cgcggccgcc ggtctgtttc ttgtagcgtc cctgcgcctg
    14761 ggccgagccc tggatcgtct ccatgaacgg caccttcggc gcgcgcgcct cgacctcgac
    14821 gccgtaacgc cggtgtatcc tctccagcgc gaactcgacg tgcatctggg cgagcccggc
    14881 gagtatgtcc tcgcccgttg actcggagcg ctccagcttg agcgacgggt cctcgtccac
    14941 gacccgcttt atcgcctcga agaccttctc ctcctcgccg cgcgtctgcg ccccgaccgc
    15001 gaacgccgtc gtcggctccg gcagttcgac cggctcgaag gtgatcgggt tctccggctt
    15061 gcagagcgtg tcgaaggtgt gggtatcccg cagcttggcg accgctacga tatctccggc
    15121 gacggcctcg tcaaccggcc tgcgctcctt gccgacgagg tgcgagacgc tcccgagcct
    15181 ctcggaagag ccggtgcgcg ggttggtcag cgcctcgtcc gagcggcagc gcccgctcac
    15241 cacgcgcagc acgctcaggc gtccggcgaa cgggtccacg tatgtcttga agacgtaagc
    15301 ggcgaagggg ccgtccgggt cgcagggaac ctcgtctcca tcctcggaga tccagcgcga
    15361 gcggtcaacg gggctggggg cgctgccggc gatcacgtcc aggagccggt ccaccccgat
    15421 catgcgctcc gcgctcgccg ccaacaccgg gatgatgagc ccgtccgcga tgcccttcct
    15481 tatcccctcg aaggtctcct cggtcgtaag gtcctcacct tcgaggtact tctccagcaa
    15541 cgtgtcgtcg ctctcggcga tggcctcgaa gagcgcgacc ttcgccgcgt ccacctcgtc
    15601 ctccatgccg gcggggatct cctcgctctg cttcccatcc acgaacgcga tgccactgag
    15661 cagcccgtac acgccccgaa ggtcgttctc gcggcctata gggaggttca gcggcacgat
    15721 ggactgcccg aaccggcctc gcagttgctc gacgaccccc atgaagtccg tgcgctcgcg
    15781 gtcaaggtgg ttgaccacga ccatgtgcgg gatctcttct ctctcccccc gccgccacac
    15841 gcgctctgtc accacctgga tcgggtcctg cgcgtgtacc accagcaccg cgcaatcggc
    15901 aacccgctgc gccacgaagg cgtccgcgac gaatccccca tcccccggcg tatccaggac
    15961 attgacctgc cgtcccttcc actccaggca ggccacgctc atcccgagcg tcatgccgcg
    16021 ctccacctct tcatccgagt agtccagaac gccgccctga gagccactcc tcccggatgc
    16081 aacccccagc atcgcctccc ccagcgacgt cttgccacta ccccggtggc cgatcaagca
    16141 tacattacga atcaacgccg gctctactgc cacgagatcc tcctttcctg cgcctaaccc
    16201 ctgccgccca ctccgcgggg cggggcatcc agcttaacgc agagtcggcc ctcgcgcagc
    16261 cgccggatgc tacaatccat tgcgagctat ggaccgtacg tctctcacag cagatatcga
    16321 ggcccccgtg ggaactttcg cgggcaacac aggcattacc aggacgtggc ccgtgctggt
    16381 ggtgctggtc tcgggtctcc tcacttactt cgcgccgcac gacgaggcgt atgcgggcgc
    16441 ggactacggg atctcgctgc tcgccaccct gggagccgcc ggctgcatcg ggtatctggc
    16501 atcccggatt tcgctgcgcc tggacctcct tccgcgtctg cggcccgcgg catccggtct
    16561 cctgccgtca gccctcacca cggccgggcc ggagacgggc gaagactggc ccgtgctcgt
    16621 cgccctgctc ttcgggctct tcctcatgct gctcgccggc ctcggcggct gggtctaacc
    16681 tactccggcg tcccgccgca acgggacccc aggcgcccaa cgggctctcc tggtccgcaa
    16741 cgttcgcggg cggaggttca cgggcacacg ccgtactcac gcgagaccca ggcaaggaag
    16801 taatcaatgg tagacctatt aaagaccatc ttcggtccct tgaccgatct gctcagcggc
    16861 acgctccaga catttcacgg gtggggggcg ccgtggtggc tctccatcat catgctgacg
    16921 gtgatcgtgc ggaccctcct cttcccgctc accttcaagc aggtaaagag catgaggaag
    16981 atgcaggagc tcaagccgga cctggatgag ataaaggcca aacacaagaa cgatcccaag
    17041 aagcagcagg aagagacgat gaagctctac ggcgagcgga gcgtcaaccc gctcggcggc
    17101 tgcctgccgg tcctcgtcca gatgccgatc ttcattaccc tgtactacac gatcaagtcc
    17161 ttcgagcacc tggagagctt caggaccggc ggtctcctgt ggttcccgga cctcacggtc
    17221 tccgacccgt acttcatcct gcccgtcctc tacgtgctga cgatgatggc ctcgcaggag
    17281 atcacgatcc gcaacaccgc gcccgaacaa cgccagctaa tgcgcttcct gccactcgtc
    17341 ttcggcggct tcctcgcgtt ctccggcttc ccggcgggcc tcttcgtata ctgggtcgcc
    17401 tccaactgca tcaccttcac ccagaactac gtcatctacc atcgccatcc gcacaccgcc
    17461 ccggcgaaga acggttccga agacgccaaa ggcgccaagg acactacccc aaaaccagag
    17521 tccgctccga aacccggcaa cgaaaaatct cccgcccaga agaccaccgc tgccacccag
    17581 aacgccccga gaagcgcccg ctcccggaac aggaaaaaga agagcgcggg caagaagagg
    17641 tagaaatcct cacccgatat ccccgcggcc gctaaccagc ggtcgccact accggcggag
    17701 cgagagttgg gctatcctac gcagggtcgg ggagagaaat agaaggagga gcagatgact
    17761 accggacaga gcgcccagac cgaacagcac ccgctgcaca gcgaccgcgg caggaccacc
    17821 atagccagca gcgtggtatc cagcatcgtg aacgtggccg ccggagaggt ggacggcctg
    17881 gcgatgtcgc acggcgggac ccgccttccg ggagacaact ctccgaccgt cggcgagttc
    17941 ttcgaccgcg taagcggcgg cggcagccgg acccgcggtg tctcggtgga agtcggtgag
    18001 cgcgaagcgg ccgtggacct caccgtgaac gtcgtctacg gtaaacccat cgcccaggtc
    18061 acccaggccg tccgtcagaa catcatcaac cgcgtggaga gcctaaccgg cctcgatgtc
    18121 acagaggtaa acatcaccgt caacgacgtg accttcccgc agcagtagtt tttcaacagt
    18181 cagaacagat actacagccg cacttgtgat gctaggctga acacgccggt agcaaggcgc
    18241 cgattttctc gctccgagct ccgccggagg gtcctcgcct acgcaagctg gcttacccga
    18301 ggacgaccag aagaatacct attagcaaag gcggtaggta gtggaaagcc ggaagagaat
    18361 caccggtgat gatcagcaag gtcagcattg cgatgctgaa gataagcgta gcccacccgg
    18421 cccatgcaag caagagcctg acctgaagca aggaccccct ccataggccg ccagagcgag
    18481 gtaaccgaca acgtcataga catagaacag cgcgaacgcc cactgagcca gcggctcgta
    18541 ataagtcggt accgtacccg tcgcgactgt cacctgagcc gccctaatca tgataacagc
    18601 acgagttttc aggacgttcg cctctcgcta ttgagaacct cacccctggc ggcttcgaag
    18661 ggcgccgcaa ccacaggtat gttgccaaag cgcaggagag tctatattgt ctgtggcttc
    18721 gcctccgctt gcctcggatg tgggtagggg tactctgcct tgtatccaag cagtcttcca
    18781 atcgaagcca acgatccgaa gagcagcgtc tgcaccagcc gcgggggccg cgccaggata
    18841 atctcattcc gatacgcatg catcatcacc cccagccgca acaggttcgg caatcccgtc
    18901 ttcggactta ccttaccgtc c
//
LOCUS       DASSMB010000150        14979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_34439, whole genome shotgun sequence.
ACCESSION   DASSMB010000150 DASSMB010000000
VERSION     DASSMB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14979
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34439"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="iolC"
                     /locus_tag="VFJ72_08925"
     CDS             complement(<1..872)
                     /gene="iolC"
                     /locus_tag="VFJ72_08925"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: protein motif:HMM:TIGR04382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HET7479621.1"
                     /translation="MTEFEVITMGRVSVDLYPEQIGATLAEVTSFAKSLGGSATNVAV
                     AAARLGHPSAVITKVGDDGFGPYVREALEGFGVDARWVGTDPALRTPIVFCEIHPPDD
                     FPLLFYREPKAPDMNLALEDLDLDAITAVRLFWTTGTGLSDEPSRSATLAALDARSTV
                     PGGITVHDLDYRPTLWKSAEEARKYNREALRLATVTVGNQDEVEMAVGVRDPLEASAA
                     LLELGVELAIVKQGPRGVLARTADGVVEVPPIPVEVVNGLGAGDAFGGALCHALISGW
                     DPERTIRYANAAGA"
     gene            complement(869..1771)
                     /locus_tag="VFJ72_08930"
     CDS             complement(869..1771)
                     /locus_tag="VFJ72_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7479622.1"
                     /translation="MTDPATTPLIEAQGISKYFGQVIALQDISMRVSAGEVMCLLGDN
                     GAGKSTLIKILSGVHQHDEGKLLIEGEEVRFNSPREALDRGIATVFQDLATIPLMSIS
                     RNFFLGREPTKGAGPFKRFDAATADRVTREEMHRIGIDVRDPSQPVGTLSGGERQSVA
                     IARAVYFGAKVLILDEPTSALGVKEAAVVLRYIAQAKERNLGVIFITHNVHHAYPIGD
                     RFTILNRGRSYGTFEKSEVSREEVVTMMAGGEEMDRLTAELEEFERSDRETEKQRSNG
                     GSHLADAFREDAEETRHGEGQERV"
     gene            complement(1771..2793)
                     /locus_tag="VFJ72_08935"
     CDS             complement(1771..2793)
                     /locus_tag="VFJ72_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479623.1"
                     /translation="MATATRPADERLRSSGVLRKLLNRPELGAVAGAILVFAFFAIVA
                     GDSGFLTSVGAISYLEVSAQLGIIVIPVAMLMIAGEFDLSVGSMIGAAGILIALPTAE
                     YGWPLWAALLLAFAVALLVGFINGYLVVTTGLPSFIVTLASLFALRGATIGFTRLITG
                     RTQVSGVQSDGFFAGIFGGEVAGVPVSIFWWVGLGILGTWILLRTTFGNWIFGTGGDP
                     VAARNVGVPVRRVKILLFMATACSATLLAAITVLDAGSADVLRGTLREFQAIIATVIG
                     GVLLTGGYGSVIGPMFGALIFGMVSQGIFFTGVNTDWFQVFLGAMLLIAVLVNNYIRK
                     KASEAR"
     gene            complement(2854..3843)
                     /locus_tag="VFJ72_08940"
     CDS             complement(2854..3843)
                     /locus_tag="VFJ72_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET7479624.1"
                     /translation="MRRAVRIQASILLALAVVFVAAGCGGGQSAQQGGGAASKGRGDI
                     KISVVTHGQASDPFWSVVKNGVDQASKDMGVDVKYSAPDTFDMVKMSQLIDAAVASKP
                     DGLAVSVPDPDALKDSIKKATDAGIPVVTLNSGADVSKQLGALTHVGQTEFKAGVGAG
                     EQLAKAGVDNALCINQEVGNAALDQRCDGFEKGLGGPVKVISVDLNDPTDAQAKIQTA
                     INQNSDANGMLTLGPTGAEPALKALDKSGKAKDMKLATFDLSPAVLEAVRDGKLVFAV
                     DQQQYLQGYLPIVFLTNYAQYGVIPKSEVLTGPAFVTKDNAKQVISLSKKGIR"
     gene            complement(3915..4793)
                     /locus_tag="VFJ72_08945"
     CDS             complement(3915..4793)
                     /locus_tag="VFJ72_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7479625.1"
                     /translation="MSQRRVAGAPISWGVCEVPGWGYQMNAERVLSEAADLGIRAVEA
                     GPDGFLPPDPEAASGLLRRYGLELVGGFVPAVLHRPETREAEMASVERQAKLFAAAGG
                     GVLVLSASTGMEDYEESVDLGKREWDELFASVAAVEEIGVRHGLSVVLHPHFGTVVER
                     PHQIERFLEGCDTEICLDTGHVMVGGGDPVALVEGAAGRIGHVHLKDVDRGLAVAVAA
                     GRLGYEEAVRRGMYRPLGEGDVELRLVVECLDRERYGGWYVLEQDLMLEAEPGVGDGP
                     IEDVKKSLAFLEEVGA"
     gene            complement(4790..5785)
                     /locus_tag="VFJ72_08950"
     CDS             complement(4790..5785)
                     /locus_tag="VFJ72_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7479626.1"
                     /translation="MRVGLLGAGRIGALHARTLAGNPRVDEVVVGDVDAARAKALAVE
                     LGGEYGTIEEVLGDGLDAVAIAASTSAHADLIRAAMEAGIPAFCEKPVALGYDETVEI
                     VEKVESSGAVLQIGFQRRFDAGYVEAKRRIDDGTVGTIYSVRLATHDPEPPHEGYIPT
                     SGGIFRDLHIHDFDVLRWLTGGEVEEVYAAGSVRKFDMFGKYGDVDTSAAMIRMRDGV
                     VAVLTGGRHDPLGYDVRAEVFGSGDSIAVGVDKHTPLHSVEPDAAAANGAMYANFQVR
                     FERAYRAELEHFVRLVGGEVENPCTASDALEALKIAMAADLSIAQDRPVKMSEIG"
     gene            complement(5923..6660)
                     /gene="nagB"
                     /locus_tag="VFJ72_08955"
     CDS             complement(5923..6660)
                     /gene="nagB"
                     /locus_tag="VFJ72_08955"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET7479627.1"
                     /translation="MEIQVLPDYDAVSEAAADVVARTLRAKPGAVLLIPTGTTPLGMY
                     RKLVELHREQGLSFEAATFFNLDEYLGLAPDHPASYHVYMSENFYGLVDADPERIHVP
                     EGAAPDPEAECERYEAAIREAGGVDLCVLGIGRNGHIGFNEPGASFESRTRVVRLAES
                     TRLVNAADFEERRAPERAITVGMKTIFESRRALLLASGSNKARAVRAAAHGPVTEEVP
                     ASVLRRHPDTTFLLDRDAALLLDDSAS"
     gene            complement(6674..7123)
                     /locus_tag="VFJ72_08960"
     CDS             complement(6674..7123)
                     /locus_tag="VFJ72_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET7479628.1"
                     /translation="MEVLAPISGRAVPLSEVPDEVFAEGMAGEGGAIVPNGSGEAVAP
                     VSGRLMKLFEGGHAFGIATEEGVELIVHLGLDTIEMQGGAFEKLAAEGDDVRAGQPIV
                     RYDVDAIRGAGYDPVTPVVVTNADEHPIRDVKTGEVSAGDLLFEVST"
     gene            complement(7123..8712)
                     /locus_tag="VFJ72_08965"
     CDS             complement(7123..8712)
                     /locus_tag="VFJ72_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET7479629.1"
                     /translation="MATAAADRRGSSGGWDRVLGSLQSVGRSLMLPIAVLPAAALLLR
                     LGQPDLLDISWMAAAGNAVFQNLPMIFAVGIAFGLSGGDGAAALAGLVGYLVFDGVFK
                     TLIPQVDGAPDPDITMGVLAGIIVGLVAAGLYRRFSDIRLPDYLAFFGGRRFVPIITA
                     LSALVLGVVFGLVWPPVQDGVNAVGQALVGLGALGTGLYGFFNRLLIPLGLHHILNTY
                     IWFQLGTYKGPDGPVQGEISRYLAGDPSAGYFLSGFFPIMMFGLPAACLAMIRHAQFP
                     KVAAGILLSAAFASFLTGITEPIEFAFLFVAPLLYLVHAILTGTALAVCTLLGIKIGF
                     GFSAGLIDYVLNFSKSNTTHPILLLGVGLVYAVIYYFVFSFFIKRFDLATPGRGEAST
                     GLSSDWILPESQRGPRPGAPEDREEREAAREAERRAEREGAPAGVAAGTATAERDRDD
                     VLAENVLEALGGRNNVESVEGCITRLRLFVDDPQKIDDARLKQLGAAGVVKRGKVAQV
                     VMGTQSDRIAERINRLLKGES"
     gene            8913..9326
                     /locus_tag="VFJ72_08970"
     CDS             8913..9326
                     /locus_tag="VFJ72_08970"
                     /inference="COORDINATES: protein motif:HMM:NF018611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HET7479630.1"
                     /translation="MSLFSTLLLVLGLLWLLQILGTWFQMRHYKEVLSGITRAGTEGY
                     VGVGNARARFGKGVILILVSGEDGYVTRALRMRGRTVFARFAELPELVGKHLDELRDE
                     GQVARNEAATMLAARKAVEQIDRIRAEREVNVEAR"
     gene            9367..9987
                     /locus_tag="VFJ72_08975"
     CDS             9367..9987
                     /locus_tag="VFJ72_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HET7479631.1"
                     /translation="MQVIIDHLSSGTLTLGLLQDGSKALAEQGGALGALEKAAEWFIG
                     LFQAGGKTFVGFVTGIIPTLIVLLTFFYTIVNLVGEQRVQGAARFAASSIFTRYTLLP
                     ILAVFFLTNPMAYTFGTFLEERYKPAFYDSAVSFVHPPLGIFPHVNPGELFVWLGVAQ
                     GIQRLDLPLGPLAVRYILAGLIVIFIRGIVTQYITFFLARRQGVEL"
     gene            9997..11028
                     /locus_tag="VFJ72_08980"
     CDS             9997..11028
                     /locus_tag="VFJ72_08980"
                     /inference="COORDINATES: protein motif:HMM:NF015569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HET7479632.1"
                     /translation="MQGKEYRAVRIERGAQGWGGPLVIQPTAEKDKIVSVTGGGISEV
                     ARRIAELTGAEAVDGFRAPPPESEMAAVVVDCGGTARAGVYPRKRIPTINLTPVGQAG
                     PLAQFIKEDIYVSGVKPGNITAADGSEVASGTSTTSPTGVTDGPAESGAASPFASPSG
                     GREDMGGGFTGFIARIGRGMGGVVAVLFQSGRQTIDTVVRNILPFMAFVTMLIGIITA
                     SGLGDLIANTLSPLASNLGGLLVLSLIVGLPFLSPVLGPGAVIAQVIGVVVGDQIGRG
                     AIDPQFALPVLFAYNVQVGCDFVPVGLALGEAKPKTIEIGVPAVLFSRQITGPIAVLI
                     GWALSFGMY"
     gene            11038..11430
                     /locus_tag="VFJ72_08985"
     CDS             11038..11430
                     /locus_tag="VFJ72_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HET7479633.1"
                     /translation="MRDLKPIYLTEVTEIGPEVADFLDSGLLILFEAGAPPELAEIAV
                     LHAPISRREEPPKPGDVVAFGPHEFRVTAIGYKAWANVLDLGHAVFKFDGLEEPELPG
                     QIHLDGPDADKLGDVVEPGLRVEIKAAS"
     gene            11427..12683
                     /locus_tag="VFJ72_08990"
     CDS             11427..12683
                     /locus_tag="VFJ72_08990"
                     /inference="COORDINATES: protein motif:HMM:NF044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7479634.1"
                     /translation="MSLRMRLEALERAGTPIRVGLVGAGQMGRGFIAQVAGIPGMETV
                     AVADVDPQRALSAFREVGITAVEGLNGASGRPAVTGDALDLARSESVDVVVEATGVTE
                     VGARVAYEAIQERKHVVMLNAEADATVGAVLARMARSAGIVYTGSAGDEPAAVMELYD
                     FARSMGFEVVAAGKGKNNPLDPSATPDSVAEEAERKHMNPKMLAAFVDGTKTMVEMAT
                     VANAIGFAPDVPGMHGPAETDPRKLAQVFSLQEEGGILSSYSTVDYVRGVAPGVFVIV
                     RSQEGAVRETMEYLGMGAGPNHVLYRPYHLTSLETPISVARAAIYGEPGIVSGDVPQA
                     EVVAVAKRDLEAGEKLGGIGGTDYYGTVYAASEATDMLPLGLAAGARTTRPVSKGSVI
                     PRAAVELAGDSFVVNLRKLQDVAIAG"
     gene            12696..13694
                     /locus_tag="VFJ72_08995"
     CDS             12696..13694
                     /locus_tag="VFJ72_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET7479635.1"
                     /translation="MLKRYPVDYRSLTDEDHLILKVIRLYYEHDLTQAQVASRMGFSR
                     PKVSKLLSEARERGLVKIEVAEPAGDSTSLEIALEDRYGLEESLVVPTSEDREATELS
                     AGRAGATLLARACGRDTVLGLSWGVSLRALADAAVPRAFSCKKVVPLVGGMGNAKIRL
                     HSNQVCAALAEKLGVESLDLAAPAIASSPESRADLAALPGIQETLAEGERCDVAVVGI
                     GGILPSSTLVEAGYFTLEEFLGLRERGVVGDVCCHFLDLDGAPRLPGFSGRIVGITLE
                     QLRSIPHTIGIATGAEKGPGAAAVLKGGYVKSLVCDEDLAHSLVEISNEGELDERI"
     gene            13681..14637
                     /locus_tag="VFJ72_09000"
     CDS             13681..14637
                     /locus_tag="VFJ72_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7479636.1"
                     /translation="MRGYKDRRELLEAMLLIRHFDEKVNELYAEGKVHGTAHFYVGQE
                     AVAVGVISALKEGDVITSTHRGHGHAIAFGLDVDRMASELLARADGYCHGKGGSMHIA
                     DVGAGMLGANGIVGGSMGIVCGAGWAFKRRKEDRVAVCFFGDGAVAEGIFHEALNLAA
                     IWKLPVVFVCENNQYGMSMSVKKAYATERISDRASGYGMPGATVDGQDLDAVNAAASE
                     AIERARSGGGPTLLEALTYRYLGHSKSDANLYRTREEITMWREKDPIVRFAGTLEEEG
                     VLQNGGLRAMDDDARKTIETALEKAQREAEPEPESALEDVYA"
     gene            14630..>14979
                     /locus_tag="VFJ72_09005"
     CDS             14630..>14979
                     /locus_tag="VFJ72_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7479637.1"
                     /translation="MPNEVGNKVREITYREALREAMVGAMREDESVFLMGEDVGVYGG
                     AFGVSRGMVEEFGEERVVDTPISEAGYVGLCTGAALVGMRPIAEIQFSDFITHCMDQL
                     VNQAAKLRYMFGGEA"
BASE COUNT         2580 a         5043 c         4852 g         2504 t
ORIGIN      
        1 atggctccgg cggcgttggc gtagcggatg gtccgctccg ggtcccagcc ggagatcagg
       61 gcgtggcaca acgcgccgcc gaaggcgtct ccagcaccga gaccgttaac gacctccacc
      121 ggtatcggcg gcacctcgac cactccatcg gcggtccggg cgaggacgcc ccgcggaccc
      181 tgcttgacta tcgccagctc gaccccgagt tcgaggagcg ccgccgaggc ttcgagcggg
      241 tcgcggacgc cgacggccat ctcgacctcg tcctggttcc cgacggttac tgtggcgagc
      301 cgcagcgcct cccggttgta cttgcgggcc tcttccgcgg acttccagag cgtcgggcgg
      361 tagtcgaggt cgtggacggt gatgccgccg ggcacggtgg agcgcgcgtc aagggcggcg
      421 agggtcgcgc tgcggctcgg ctcgtcggag aggccggtgc cggtggtcca gaaaaggcgg
      481 acggccgtga tggcgtccag gtccaggtcc tccagggcga ggttcatatc cggggccttc
      541 ggctcgcggt agaagaggag cgggaagtcg tcgggagggt gtatctcgca gaagactatc
      601 ggcgtcctga gcgcagggtc cgtgccgacc cagcgagcgt ccaccccaaa accttccagc
      661 gcctcgcgca cataagggcc gaacccgtca tccccgacct tcgtgatgac cgccgagggg
      721 tggccgaggc gagcggcggc gacggcgacg ttcgtggcgc tgccgcccag ggacttggcg
      781 aacgacgtca cctcggcgag cgtggccccg atctgctccg ggtacaggtc cacgctgacc
      841 cggcccatcg tgatgacctc gaattcggtc acaccctctc ctggccctcg ccgtggcgag
      901 tctcctcggc gtcctcccgg aaagcatcgg cgagatgcga gccgccgttg gagcgttgct
      961 tctcggtctc ccggtccgaa cgctcgaact cctccagctc cgccgtaagc cggtccatct
     1021 cctcgccgcc ggccatcatc gtgacgacct cctcgcggct gacctcggac ttctcgaagg
     1081 tcccgtaact cctgccgcgg ttcaggatgg tgaagcggtc cccgatcggg taggcgtgat
     1141 ggacgttgtg ggtgatgaag atgacgccca ggttcctctc cttcgcctgg gcgatgtagc
     1201 gcaagacaac cgccgcctcc ttcacgccca gcgccgaggt tggctcgtcc aggatgagga
     1261 ccttcgcccc gaagtagacg gcgcgggcga tggcgaccga ctgccgctcg ccgccggaga
     1321 gggtgccgac gggctgcgac gggtcgcgga cgtctatgcc gatgcggtgc atctcctcgc
     1381 gcgtaacccg gtcggctgtc gcggcgtcga accgtttaaa cgggccggcg cccttcgtgg
     1441 gttcgcggcc gaggaagaag ttgcgggaga tgctcatcag cgggatcgtc gcgaggtcct
     1501 ggaagacggt ggcgatgccg cggtcgaggg cctcgcgtgg ggagttgaac cttacctcct
     1561 cgccttcgat caggagcttc ccctcgtcgt gctggtggac gccggagagg atcttgatga
     1621 gcgtgctctt ccccgcgccg ttatccccga gcaggcacat cacctcgccc gcgctcacgc
     1681 gcatggagat gtcctgcaag gcgatgacct ggccgaaata cttgctaatc ccctgcgcct
     1741 cgataagagg cgtcgttgcc gggtccgtca tcagcgcgcc tccgatgcct tcttgcggat
     1801 gtagttgttg acgagaacgg cgatcaggag catcgcgccc aggaagacct ggaaccagtc
     1861 ggtgttcacg ccggtaaaga agattccctg gctcaccatc ccgaagatga gcgccccgaa
     1921 catcggtcct atgacggagc cgtacccgcc cgtcagcagt acgcccccaa taaccgtggc
     1981 gatgatcgcc tggaactccc gcagcgtgcc gcgcagcacg tcggccgagc ctgcgtcgag
     2041 caccgtgatc gctgcgagca gggtcgcgga gcaggccgtc gccataaaga gcagtatctt
     2101 gacccgccgc accggcacgc cgacgttgcg ggccgcgacg ggatctccgc cggtaccgaa
     2161 gatccaattg ccaaacgtgg tccgcagcag tatccacgtc ccaagtatcc ccagcccgac
     2221 ccaccagaag atagagacgg gcacccccgc gacctccccg ccgaagatac cggcgaagaa
     2281 gccatcagac tgtacgcctg agacctgggt gcgccccgtt atgagccgcg tgaacccgat
     2341 ggtcgctccg cgcagcgcga agagcgaggc aagcgtgacg ataaacgagg gcaacccggt
     2401 cgtgacgacc aggtagccgt tgatgaaccc cacgaggagc gccaccgcga aagccaacag
     2461 cagggcggcc cacagaggcc atccgtattc cgccgtgggc agcgcgataa ggatgcccgc
     2521 cgccccgatc atggacccca cggagaggtc gaactcgccc gcgatcatga gcatcgccac
     2581 cgggatgacg atgatgccta gctgcgccga gacctccaga tagcttatgg ctccgacaga
     2641 ggtcaggaac ccggaatccc cggcgacaat agcaaagaac gcgaagacca ggatagcccc
     2701 ggcgaccgcg ccgagctccg ggcggttcag gagtttcctc agtaccccgg agctgcggag
     2761 ccgctcgtcg gcgggtcggg tagcggttgc catcgctcgt actcctttcc cggcgtgggg
     2821 agcgaacgcg cgtccgcccc ccgcacgttg ctgctagcgg attcccttct tgctgaggct
     2881 gatgacctgc ttggcgttgt ccttcgttac gaacgccgga ccggtcagga cttcggactt
     2941 gggtatcacc ccgtactgcg cgtagttcgt caggaagacg atgggcaggt agccctggag
     3001 gtattgttgc tggtcgacgg cgaagaccag cttaccgtcg cggacagcct caagcaccgc
     3061 cggggagagg tcgaaggtgg cgagcttcat atctttcgcc ttcccgctct tgtccagggc
     3121 cttcagcgcc ggctccgcgc ccgtcgggcc gagagtcagc atcccgttgg cgtcggagtt
     3181 ctggtttatg gcggtctgga tcttggcctg cgcgtccgtc gggtcgttga ggtcgacgga
     3241 aatgaccttt accggccccc cgagcccctt ctcgaacccg tcgcagcgct gatcgagcgc
     3301 ggcgttaccg acttcctggt tgatgcacag cgcgttgtca acgccggctt tggcgagctg
     3361 ctcgccagct cccacgccag ccttgaactc cgtctgcccg acgtgcgtta gcgccccaag
     3421 ctgcttgctc acgtcagccc cggagttgag cgtgacgacc gggataccgg cgtcggtggc
     3481 cttcttgatc gagtccttca gcgcgtccgg gtccggcacc gagacggcga gaccatccgg
     3541 cttcgaggcc acggcagcgt cgatgagctg gctcatcttg accatgtcga aggtatcggg
     3601 cgcactgtat ttgacgtcaa cgcccatgtc cttcgacgcc tggtccaccc cgttcttgac
     3661 cacggaccag aacgggtccg aagcctgccc gtgggtcact accgatatct ttatgtcgcc
     3721 tcgccccttg gacgccgcgc cgccgccctg ctgggcgctc tgccctccac cgcaaccggc
     3781 cgccacgaac accaccgcca atgccagcag gatactggcc tggatcctaa ccgctcttct
     3841 caccccttct cctttccata taggccaccc cggcctcttt tccccacgct attacggtct
     3901 ccttgcatcg agagtcaggc acccacctcc tccaggaatg cgaggctctt cttcacatct
     3961 tcgatggggc catccccaac gccgggctcg gcctccagca tcaagtcctg ctccagcaca
     4021 taccagccac cgtacctttc cctatccagg cactctacca ccagccggag ctccacgtct
     4081 ccctctccga gcggccggta catcccgcgc cgcacggcct cctcgtagcc gagccttccg
     4141 gccgctaccg ctaccgcgag accgcggtcc acgtccttta gatggacgtg cccgatgcgt
     4201 cccgccgctc cctccaccag cgccaccggg tctccgccac cgaccatcac atgtccggtg
     4261 tccaggcata tctcagtgtc gcagccctcc aggaaccgct ctatctgatg cggcctctcg
     4321 acgacggtcc cgaagtgcgg gtgcagcacc accgagaggc cgtgccgtac cccgatctcc
     4381 tcgaccgccg ccaccgatgc gaacagctcg tcccactccc ttttgccgag gtccacgctc
     4441 tcctcgtagt cctccattcc cgtcgaggcc gataacacca gcacgccgcc gcccgccgcc
     4501 gcgaatagct tcgcctgccg ctccacagac gccatctccg cctcccgcgt ctccgggcga
     4561 tgcagcacgg cgggcacgaa cccgccgacc agttcgaggc cgtagcgccg cagcagccca
     4621 gacgccgcct ccgggtccgg cggcaggaaa ccgtccgggc cggcctcgac tgcccggatg
     4681 ccaagatccg ccgcctcgct cagcacccgc tcggcgttca tctggtagcc ccatcctggg
     4741 acctcgcaca cgccccagga gatcggggct ccggccaccc gccgctggct caccctatct
     4801 ccgacatctt caccggccgg tcttgagcga tggagaggtc cgcggccatc gctatcttga
     4861 gagcctccag cgcatcgctg gccgtgcagg ggttctccac ctcgcctccc accagcctga
     4921 cgaagtgctc cagctcggcc cggtatgccc gctcgaagcg gacctggaag ttcgcgtaca
     4981 tcgccccgtt ggcggccgcc gcgtccggct cgacggagtg caggggagta tgcttatcca
     5041 cgccgaccgc gatgctgtcc cccgaaccga agacctccgc ccgcacgtcg tagccgagcg
     5101 ggtcgtgccg tccgccggtc agcacggcca ccacgccatc gcgcatccgg atcatggccg
     5161 ccgaagtgtc cacgtcgccg tacttcccga acatgtcgaa cttcctcacg ctccccgccg
     5221 catacacctc ctcgacctcc ccgcccgtca accagcgcag aacgtcgaaa tcgtggatgt
     5281 gcaggtcccg gaagatgccg cccgatgtcg ggatgtagcc ctcgtgcggc ggctcgggat
     5341 cgtgagtggc gagcctgacg gagtagatcg tgccgaccgt cccatcgtct atccggcgct
     5401 tcgcctcgac gtaccccgcg tcgaagcgcc gctggaagcc gatctgcaac acagcccccg
     5461 acgactcgac cttctccacg atctcgaccg tctcgtcgta gccgagcgcg accggcttct
     5521 cgcagaacgc cggtatcccg gcctccatcg ccgcccggat gaggtccgcg tgcgccgacg
     5581 tggaggccgc tatagccacc gcgtcgagac catcgcctag cacttcctct atcgtgccgt
     5641 attcgccgcc caactccacc gcgagcgcct tcgcgcgcgc cgcatccaca tcccccacga
     5701 caacctcgtc cacccgcgga ttacccgcga gcgtccgagc gtgcagagcc ccaatcctac
     5761 cggccccgag cagcccaacc ctcatgcgca cctctcccga aatatgtccc gcgtcctcgt
     5821 cttcaccctg ttttggaacc ttccaaaaca cgattgccat tattgtcaga ggctgaagcc
     5881 cgtgtcaaat cgaggtggcg cgcggctggt gaagaggtgc ggctagctgg cgctatcgtc
     5941 gaggagcagg gctgcgtccc tgtcgagcag gaaggtagta tccgggtggc gccgcagcac
     6001 ggaggccggg acctcttccg ttacaggacc gtgggccgcg gcgcgtacgg cgcgggcctt
     6061 gttggagccg gaggcgagga gcagggcgcg gcgcgactcg aaaatggtct tcatgccgac
     6121 ggtgatagcg cgttccgggg cgcggcgctc ctcgaagtcc gcggcgttca cgagccgggt
     6181 gctctccgcg aggcgcacga cgcgggtccg ggactcgaaa gacgctccgg gctcgttgaa
     6241 ccctatgtga ccgttgcggc cgattccgag cacgcacagg tccacgcccc ccgcctcccg
     6301 aatggccgcc tcgtagcgct cacactcggc ctctgggtcc ggcgcggcgc cttccgggac
     6361 gtggatccgc tcggggtcgg cgtccaccag gccgtagaag ttctcactca tatagacgtg
     6421 gtaactcgcc gggtgatcag gggccaggcc caggtactcg tccaggttaa agaacgtcgc
     6481 ggcctcgaag gagagtccct gctcccggtg cagctcgacc agtttccggt acatcccgag
     6541 cggggtggtg ccggtcggga tcaggagtac agcgccgggc ttcgcgcgca gggtccgcgc
     6601 cacgacatcg gcggcggcct cgctcaccgc gtcgtagtcg gggaggacct ggatctccaa
     6661 agctggctcc gctctaggta gagacctcga agagcaggtc accggcgctc acctctcccg
     6721 tctttacgtc tctgataggg tgctcgtcgg cgttggtgac gacgaccggc gtcacggggt
     6781 cgtagcccgc gccccggatg gcgtccacgt cgtagcgcac gatgggctgt ccggcccgga
     6841 cgtcatctcc ctccgcggct aacttctcga acgctccgcc ctgcatctcg atggtgtcga
     6901 ggccgaggtg gacgatcaac tccaccccct cctcggtcgc tataccgaag gcgtgcccgc
     6961 cctcgaaaag cttcatcagc ctgccggaga cgggagctac cgcctcgccg ctaccattgg
     7021 ggactatggc gccaccctcg ccggccatcc cctcggcgaa gacctcatcc gggacctccg
     7081 agagagggac tgcgcggccg gatatggggg cgagtacctc cactagctct cccccttgag
     7141 gagacggttt atcctctcgg caatgcggtc ggactgggtg cccatgacca cctgagcgac
     7201 cttcccgcgc ttgaccacgc cggcggctcc gagctgcttg agccgagcgt cgtcgatctt
     7261 ttgcgggtcg tccacgaaga gccggagccg cgtgatgcac ccctcgacgc tctccacgtt
     7321 gttcctgcct cccagcgctt ccagcacgtt ctccgcgagc acgtcgtcgc gatcgcgttc
     7381 ggccgtggcc gtcccggcgg cgactccggc gggcgcacct tcgcgctcgg cccggcgttc
     7441 cgcctcgcgc gctgcctcac gctcttcccg atcttctggc gctccgggac gtggaccgcg
     7501 ctggctctcg gggaggatcc agtccgagga aagcccggtg gacgcctctc cgcggcccgg
     7561 tgtcgccaga tcgaaccgct tgataaagaa gctgaagacg aagtagtaga tcactgcgta
     7621 gaccagcccc acgccaagca ggaggatcgg gtgcgtggta ttcgacttgc tgaagttcag
     7681 cacgtagtcg atgagtcccg cggagaaacc gaaccctatc tttatgccga gcagcgtaca
     7741 caccgccagc gccgtgcccg tcagtatcgc gtgtaccagg tagaggagcg gagcgacgaa
     7801 caggaaggcg aactcgatgg gctccgtgat gccggtcaga aacgaggcga aggccgccga
     7861 gagcaagata cccgccgcga ccttcgggaa ctgagcgtga cggatcatgg cgaggcaagc
     7921 agccggtaga ccgaacatca taatcgggaa gaacccggag aggaagtaac cggcgctggg
     7981 atccccggcg aggtagcgcg agatctcacc ctgcaccggc ccgtccggtc ccttgtaggt
     8041 cccgagctgg aaccagatgt aggtgttgag gatgtggtgc aaccctagag ggatgagcag
     8101 gcggttgaag aacccgtata gacccgtccc cagcgccccg agtcctacca gcgcctgtcc
     8161 caccgcgttt accccatcct gtaccggagg ccagacgaga ccgaagacaa cacccaggac
     8221 cagcgcggag agcgcggtta tgatcggcac gaacctccgg ccaccgaaga acgccagata
     8281 gtccggcaaa cggatgtcgg agaagcgccg gtacaacccg gccgccacga gacccacgat
     8341 tattccggcg agcacgccca tcgtgatatc cgggtccggc gctccgtcca cctgcggtat
     8401 gagggtcttg aacaccccgt cgaataccag gtaaccgacg agtcccgcga gcgccgcggc
     8461 tccgtctcct ccggagagcc cgaaggctat gccgacggcg aagatcatgg gcaggttctg
     8521 gaagactgcg ttcccagccg ccgccatcca tgagatgtcc aggaggtccg gctgccccaa
     8581 cctcaacaat agcgccgccg ccggaagcac cgctatcggt aacatcagcg agcgtccaac
     8641 gctctgcagg gaccccaaaa cccggtccca cccgccggaa ctacctcgtc tatcagcagc
     8701 cgctgtagcc atgtgttctc cttccggaaa ctgcaaaccc accctacgga ccggactcta
     8761 ctactatcgc gccccccagg caatgattcc gctcccgacc atgcttccag attatttttg
     8821 acttttgttc ttgactttga caaaagtcac gtatatcgtg ttgcgaactt cacggggaag
     8881 atggagatat tgaacaggga aggagagtaa aggtgagttt gttttcgaca ctgcttctgg
     8941 tgctcgggtt gctgtggctg ttgcagatcc tcggcacgtg gttccagatg cgtcactaca
     9001 aggaggttct ctcggggatc acacgggccg gcactgaggg gtacgtcggg gtggggaatg
     9061 cgcgggcgag gttcgggaag ggtgtgatct tgatcctggt gtccggcgaa gatggatacg
     9121 tcacgcgggc gctcaggatg cgcggcagga cggtgttcgc caggttcgcg gagctgccgg
     9181 agcttgtggg gaagcacctc gacgagttgc gggatgaggg tcaggtcgct cggaacgagg
     9241 cggcgacgat gctcgcggcc aggaaagccg tggagcagat agacaggatc cgggccgagc
     9301 gggaggtgaa cgtggaggcg cgttagagac gcagcgagtt gaaagctagg acgctaagga
     9361 gagtacatgc aagtaatcat tgatcatctg tcttcaggga cattgacgct cggcttactc
     9421 caggacggct cgaaagccct cgccgaacag ggcggggcgc tcggcgctct ggagaaggcg
     9481 gcggagtggt tcatcgggct gttccaggcg ggcggcaaga cgttcgtcgg gttcgtaacg
     9541 ggcataatcc cgaccctgat cgtgctgttg acattcttct ataccatcgt gaacctggtc
     9601 ggcgagcagc gggtgcaggg agccgcccgg ttcgcggcga gcagcatatt cacacgctac
     9661 acgttgctgc ccattctggc ggtcttcttc cttacgaacc cgatggcata caccttcggg
     9721 acgttcctgg aggagcggta caagccggcc ttctacgact cggcggtctc gttcgtgcat
     9781 ccgccgctcg gcatcttccc gcacgtcaat ccgggagagc tgttcgtctg gctcggtgtc
     9841 gcccagggga ttcagaggct cgacctgccg ctcgggccgc tggcggtccg gtacatactc
     9901 gcgggtctta tcgtgatctt catccggggc atcgtgacgc agtacataac tttcttcctg
     9961 gctcggcgtc agggtgtaga gctgtagggg gtggagatgc agggtaagga atacagggca
    10021 gtaaggatag agcgcggcgc ccagggatgg ggcggcccgc tcgtgatcca gccgacggcg
    10081 gagaaggaca agatcgtgtc cgtaaccggc ggcggtatct cggaggtggc ccgcaggata
    10141 gcggagctga ctggagcgga agcggtggac ggctttcgcg ccccgccccc cgagagcgag
    10201 atggcggccg tggtcgtgga ctgcggcggg acggcgcggg ccggggtgta cccccgcaag
    10261 cgtatcccga ccatcaacct gacccccgtg gggcaggccg gaccactcgc gcagttcata
    10321 aaagaggaca tctacgtctc cggggtaaag cccggcaaca ttaccgccgc cgacggaagt
    10381 gaagtggcct ctgggacctc tacaacctcc ccgaccggcg ttacggacgg tccggccgag
    10441 tccggggcgg cgagcccgtt cgcgtcgcca tcgggaggcc gggaagatat gggcggcggc
    10501 ttcaccggct tcatcgcgcg catcgggcgc ggcatgggcg gcgtcgtggc ggtgctgttc
    10561 cagagcggac ggcagaccat agataccgtg gtccgaaaca tcttgccgtt catggcgttc
    10621 gtcaccatgc tcatcggcat cataacggcc tcgggacttg gagatttgat cgccaacacg
    10681 ctctcgccgc tggcgagcaa cctcggcggc ctgctggtgc tgtccttgat cgtcggtttg
    10741 ccgttcctct cgccggtgtt ggggcctgga gccgtcatcg cgcaggtcat cggagtggtg
    10801 gtaggagacc agatcggacg cggcgccata gacccgcagt tcgcattgcc ggtcctcttc
    10861 gcctacaacg tgcaggtcgg ctgcgacttc gtacccgtag ggctcgcgct cggcgaggcc
    10921 aagcccaaga ccatcgagat cggcgtcccg gcggtgctct tcagccgcca gatcaccggc
    10981 cccatcgcgg tgctcatcgg ctgggcgctc agcttcggga tgtactagga aaggttgttg
    11041 cgagacttga aacctatcta cctgacggaa gtcacggaga tcgggccgga agtcgcggac
    11101 ttcctggatt cggggctctt gatcctgttc gaggcgggag ccccgccgga gctggccgag
    11161 atcgcggtgc tgcacgcccc gatctcgcgc cgcgaggaac ccccgaaacc cggcgacgta
    11221 gtcgcgttcg gcccccacga gttccgcgtt acggccatcg gctacaaggc gtgggcgaac
    11281 gtgctggacc tcggccacgc ggtattcaag ttcgacggac tggaggaacc agagttgcca
    11341 ggacagatcc acctcgacgg cccggacgcc gataagctcg gcgacgtcgt agaaccgggg
    11401 ctgcgggtcg agataaaggc cgcctcgtga gcctgcggat gcggctcgaa gcgcttgaac
    11461 gcgctgggac cccgatccgg gtcgggctcg tcggcgcggg ccagatggga cgcggcttta
    11521 tagcccaggt cgccggcatc cccggcatgg agaccgtcgc cgtcgccgac gtcgacccac
    11581 aacgcgccct gagcgccttc cgcgaggttg gcattacggc cgtcgagggc ctgaacggag
    11641 cctccggccg gccagccgtt accggcgacg ccctggacct cgcccggtcg gagagcgtag
    11701 acgtcgtcgt ggaggcgacc ggcgtaacgg aggtcggtgc gcgggtggcg tacgaagcca
    11761 tccaggagcg caagcacgtc gtcatgctca acgccgaggc cgacgctacg gtcggggcgg
    11821 tgctcgcgcg catggcccgc agcgccggga tcgtctatac gggctcggcc ggggacgagc
    11881 cggccgccgt catggagctc tacgacttcg cccgctcgat gggcttcgag gtcgtcgcgg
    11941 cgggcaaggg caagaacaat ccgctcgacc catcagccac ccccgattcg gtggccgagg
    12001 aggccgaacg caagcacatg aaccccaaga tgctcgccgc cttcgtggac ggtactaaga
    12061 cgatggtcga gatggcgacc gtggcgaacg ccatcggctt cgcccccgac gtccccggaa
    12121 tgcacggccc cgccgagaca gacccgagga aactcgcgca ggtcttcagc ctccaggaag
    12181 aaggcggcat cctctccagc tactccaccg tagactacgt gcgcggggtc gcccccggcg
    12241 tgttcgtcat cgtccgctcg caggaggggg cggtgcgcga gaccatggag tacctcggga
    12301 tgggagcggg cccgaaccac gtcctgtacc gcccctacca cctcaccagc cttgagaccc
    12361 ccatctcggt ggcgcgggcc gcgatctatg gcgagcccgg catagtgtcg ggagacgtgc
    12421 cgcaggcgga ggtcgtcgcc gtggcgaaac gggacctgga agccggagag aagctcgggg
    12481 gcataggtgg cacggactac tacggtaccg tctacgccgc ctcggaggcc acggacatgc
    12541 tcccgctcgg ccttgcagcc ggcgcgcgga ccacgcggcc ggtgtctaag ggaagcgtca
    12601 taccgcgagc ggccgtggag cttgccggcg actcgttcgt ggtcaacctg cgcaaacttc
    12661 aagacgtggc cattgcaggt taagcgggca ggccgatgtt aaaacggtat ccagtagact
    12721 accggagcct cacggacgag gatcacctca tcctgaaggt catccgtctc tactacgagc
    12781 atgacctgac ccaggcgcag gtggcttcgc ggatgggttt ctcgcgcccg aaggtctcga
    12841 agttactctc cgaggcccgc gagcggggac tcgtcaagat cgaagtggcc gaaccggccg
    12901 gggactctac gtcgctggag attgccctgg aagaccgcta cggcctggaa gagagcctgg
    12961 tcgtcccgac cagcgaagac cgagaggcca ccgagctatc ggcgggccgg gccggcgcta
    13021 cactcctggc gcgcgcctgc ggccgcgata cggtcctcgg gctctcttgg ggagtgtcgc
    13081 tgcgcgcgct ggcagacgcc gcggtcccga gggctttctc ctgcaagaag gtcgttccgc
    13141 tcgtcggcgg catgggcaat gcaaaaatac ggttgcactc gaaccaggtt tgcgcggcgc
    13201 tcgcggagaa gctcggcgtg gagagcctgg acctggccgc cccggccatc gccagctctc
    13261 ccgagagccg cgcggatctg gcggcgctgc ccggcattca ggagacgctg gccgagggcg
    13321 agcgctgcga cgtggccgtc gtcggcatcg gcgggatact ccccagttct acactcgtag
    13381 aagctggcta cttcacgctc gaagagttcc tcgggctccg ggagcggggc gtggtcggcg
    13441 acgtgtgctg ccacttcctg gacttggacg gcgctccccg cctcccgggc ttctctggcc
    13501 ggatcgtcgg catcacgctc gaacagttgc gcagcatccc gcacactatc gggatcgcca
    13561 cgggagcgga gaagggtccc ggtgcggcgg cggtcctgaa gggcggttac gtgaagtcgc
    13621 tggtctgcga tgaggacctc gctcactcac tggtagaaat ctcaaacgaa ggagagctag
    13681 atgagaggat ataaagaccg cagggagcta ttggaggcga tgctcctgat ccgtcacttc
    13741 gacgagaagg tcaacgagct ctacgccgag gggaaggtcc acggcaccgc ccacttctac
    13801 gtgggccagg aggcggtggc ggtcggcgtg atctcggccc tcaaggaggg cgacgtcatc
    13861 acctccaccc accggggcca cgggcacgcc atcgcattcg gcctggacgt ggaccggatg
    13921 gcctcggagc ttctcgcccg cgccgacggc tactgccacg gcaagggcgg cagtatgcac
    13981 atcgccgacg tgggcgccgg gatgctcggg gccaacggca tcgtcggcgg gagcatgggc
    14041 atcgtctgcg gcgcgggctg ggcgttcaag cgccgcaagg aggaccgcgt ggccgtctgc
    14101 ttcttcgggg acggcgcggt ggccgagggc atcttccacg aggcccttaa cctcgcagcc
    14161 atctggaagc tgccggtggt cttcgtctgc gagaacaacc agtacgggat gtccatgagc
    14221 gtgaagaagg cctacgccac ggagcgcatc tccgaccgcg cctccggcta cgggatgccc
    14281 ggcgccaccg tggacggcca ggacctcgac gcggtcaacg ccgccgcctc cgaggccatc
    14341 gagcgcgcca ggagcggggg cggccccacg ctcctcgaag ccctcaccta ccgctacctc
    14401 ggccactcca agagcgacgc caacctctac cgcacccgcg aggagatcac catgtggcgc
    14461 gagaaggacc ccatcgtccg cttcgccggc accctggagg aagaaggtgt cctgcaaaac
    14521 ggcgggctcc gggctatgga cgatgatgcg aggaagacca tagagacggc cctggagaaa
    14581 gcccagaggg aggccgaacc cgagccggag agcgcgctgg aggacgtgta tgcctaacga
    14641 ggtcggtaac aaagttcgag agatcacata ccgcgaggcc cttagagagg cgatggtcgg
    14701 cgcgatgcgc gaggatgagt ccgtcttcct catgggcgag gatgtcggcg tgtacggcgg
    14761 cgccttcggc gtgagccgcg gcatggtcga ggagttcggc gaggagcggg tggtggacac
    14821 tcccatctcg gaggccggct acgtcgggct ctgcaccggc gcggcgctcg tcgggatgcg
    14881 ccccatagct gaaatacagt tctcagactt catcacccac tgcatggacc agctcgtgaa
    14941 ccaggctgcc aagctccggt acatgttcgg cggcgaggc
//
LOCUS       DASSMB010000151        23757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1049271, whole genome shotgun sequence.
ACCESSION   DASSMB010000151 DASSMB010000000
VERSION     DASSMB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 23757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23757
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049271"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..649
                     /locus_tag="VFJ72_09010"
     CDS             128..649
                     /locus_tag="VFJ72_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7479638.1"
                     /translation="MTRAALLTTVMMLAIFLSSGVAWAAMIRCDGGVCRGTNSGDQMN
                     GSARSDRMYGLAGIDVMNGNRDNDLMYGGGAADRMFGGYGNDRIYGQYLNDMIDGGPA
                     SDRIFGGSGRDTLYGGTGNDFIFAADNQFDSIDCGGGADVAVIDFEEDQMSLNVPLID
                     YITLTRCETIRYG"
     gene            complement(720..2654)
                     /locus_tag="VFJ72_09015"
     CDS             complement(720..2654)
                     /locus_tag="VFJ72_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479639.1"
                     /translation="MRGSVIVQPVRSVSEHRFSPLPGARAWLFLLAALLASLLIGAPS
                     ASATVVAPESPVAADGASKRAPAISGDLAVWADDRGLGGGDIYIRDLSPGGGETQVTN
                     DLATQANPSISGSFVVWQDNSSGNWDIYGLDLSTLSSGGQPFEIAAGAGDQRNPDISG
                     DFVVWEDDSGGDWDVRGRTLPSGVVSDIATGAGDQRSPAVSGERVVWQQISSAGEADI
                     IFKDLFSGAQKQLNTSGGWRQAPDISGDTVVWRDERTAGDLDIYAYDLAGGGERRLTE
                     GAGDQWAPEVSGRVVVWADDRGVGSTDIYGEDISNGDEFAVSTASGPQDDPAVDGDRV
                     VWTSQRDGVDLGVYDIYGTTLDLAPAPPSGLKAVGSAGGVNLSWDVSPEVDLAGYNVY
                     RSGSPDGTYTKLNANLLSAPSYMDSDAPKGTRSYYRFTAVDSAGGESGAGRADAVAPK
                     PTEITLSSSVSTLTYNGGTTNLSGKLASGTDALAGRTVVLEMRPEDATSWSPVPNGRV
                     TTGVDGSFSLTGVRVDKSTDYRALFVSEEDELQSSTSSQMHVNVNMIISVSTSAKYVK
                     VKKFVTIYGMVLPAHTGNVQLIIKHNGAVVSRKFTLLNSSRFSTRYRPLAPGIYYVTA
                     LFGSQPNQGVTNTARFVVKR"
     gene            complement(2858..9187)
                     /locus_tag="VFJ72_09020"
     CDS             complement(2858..9187)
                     /locus_tag="VFJ72_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479640.1"
                     /translation="MVANFISAKNTNLTALARSLGLYAAATLSMTAAGIHLWVAPEHL
                     AEWWGYGAFFLITALVQGLFGVALLRWPGRRLFLAGAFGNLGIVAFYIITRTAGIPFI
                     GPHAGEVHPAAALDLFSVAVEISLVATLVALADESRRARLYLALAAVQALAAGAALHS
                     ASFGTYEAFGLGSLTHWLQTSMLALPLAALALWVSTYLSRRISTFFFGTATGFSARLT
                     WALTAAFAYALASVPADTLATQHAGAGTFAAQAGRDAGITLLAGFLVLFSIAALRGTP
                     YEAPRTVSFWHPRALAAVAGLAAGAMVVAGPSLFGAFSAPAEAQAAPDCGAATADRSY
                     DVAAINLDIPYNRWGDSDPNGQMYVLQGDKAAVKNWSKPLAADPSQDPAGNRRLRPRP
                     LVIRANAGECVRVDFTNELTDGGDGLPTNPRASMKVFGAAYDAQTSDGSAVGYNDDTT
                     VPNTPGQNKITYFWKAPNAEGLFMIRDQGATAGTEGDGGSVAHGLFGALAVEPAGSTW
                     TDPVSGNPLYTGTANQSGSLYVDADIHPQGGKSFRESVQLAQDEIPHSIGFGFNYGSD
                     QQRHREAPGNRAPDGIGEETSLSSWVAGDPAMIKLASGPGPWLPTPENTDPASVEDCG
                     IGTPGFNSTSCYISNVTHTYVGDPSKIRFALAGVKETHVFHMHAHQWLAEPADTGDAP
                     MDNITDANRPDSTTIDSQTFGPGESYTADLLFGSGSKKGTIGDSIFHCHLYPHFADGF
                     WALFRTHDVLEDGKNSTPDGVNVRNLQPLPDRTPLPAATPDNPGYPRFIPGKFGWRAP
                     QPINGISEARPDDPNTPDVDESQDDPTTVKREDLQQATRIVAGQALDPNLDPNDPNLT
                     PAQKEVADKLAVEKNVQKLHYGDRTPRPGAPLTDPCPAGAREITYNVSVMQRDITYNE
                     AGWHDTQGRFLVLDKDVPKMLEKNPDGTYKNQPEPLFFRANAGDCINYNLTNRLPNWL
                     GDDAFTNLNQTNMFGEHIHLVKFDVLGSDGSSNGWNYQEAAFTQKQMNFNKDVMNGGP
                     GAPSCNRTDGCRVPDPAPGTWNPQWSGDEPGQTIHERWYADYELRTVFTHDHHFAAVD
                     QNRGLFGALIVEPKGMDFRDPKTGAFSQPINNPANGPVCGSGADPNACTATAAGAAVD
                     VIGPGAKDDFREFGLGIQDFVSLTKKDGDPRVKADTINPPEAPEHFPDDDPGVMSVNY
                     RNAPFVLRQTDKNGNPTDPAHVFSSTVHGDPQTPVLQAYSEDPVRIRLIQGSQEEQHV
                     MTVNGMRWKEEPDDPESPFINARQLGVSEAFNFEVPKMTCGSTDQTCTGDYLYGSTGM
                     DDMYSGMWGILRARGKQVPSLKPLPDNASPTEATVAGVPTSSPISSTSSAPPESNNPG
                     TPCPAGAPVKKYEVVAMQKSLTYNKAGDNDPYGLIYVLASDEAAIKSGEKKPEPLVLR
                     ANEGDCIEVGLTNHLTPAWLQHGNAGTDGDAKAPTEPDAGTPAGLRVSLHPQLVKYDV
                     RGSDGTAVGYNKDETVAPGDRKLYRWYADDVAPGELGATNLTDFGDARGHRHHGLFAG
                     LNIEPKGATYHDPKTGDEIKSGVSADIRIPGPDNDFREFTTFFQDGLNLRDSSGVPIP
                     DRPDHPPTPAEPLAGALDFEDQGEKGFNYGNAPFDHRLGYIPMANDSPDGAGMANVFS
                     SSAHGDPDTPIFRAYAGDRVRMRVLQGEDKPREHSFQLSGHSWRAQPDDPGSTVIGTA
                     GGMSVGRALNIDLPSVGGPSGTPGDYRYGCGVFFHHLSGGLWGIMRVYGQPSPAAALN
                     PSAIGDVDDPYAGGHPLMPLDLAGIATSSNINAAPASLEPGQATTLSGTLSTANGPLA
                     KRSVTLEQKPAGETAFTAVANGSLKTDADGNFSLADVKPAKTTEYRASFAGQPTTGLQ
                     PSVSASKQVIVMTGSAITANASAAIVNYGGTTAISGALTSDGAALPGKTVVLEQKPAG
                     TAAFSAVAGATAQTGAGGSYTFAGVKPLKNTTYRVSFAGQPDAGLKATEAMVVVKVKA
                     MIGLNVAATNLKLGNSRVISGSVAPAHAGVVKLTIKRNGAPVLANKSLSLKSSRYSFS
                     YKPPRAGLYTVSVSFAGEADHLGGTSPVRSFKVIK"
     gene            complement(9315..9854)
                     /gene="lepB"
                     /locus_tag="VFJ72_09025"
     CDS             complement(9315..9854)
                     /gene="lepB"
                     /locus_tag="VFJ72_09025"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7479641.1"
                     /translation="MERGEAGKARRGRSAFDLLLVLFLAFAFMFGVVRPFVAEVFLIP
                     SESMVPTLKVGDSVLALKLAYRITDPQRGDVAVFRDPEGDLVIKRIVGLPGDTVAVRD
                     GVLYVNGERKKESYVDYELTDSTFYGPDKVPPGHVYVMGDNRSNSRDSRDYGAVPEED
                     LLGRVVMKVAPVGEAGLLR"
     gene            complement(9896..10438)
                     /locus_tag="VFJ72_09030"
     CDS             complement(9896..10438)
                     /locus_tag="VFJ72_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479642.1"
                     /translation="MNMEIKNPTTARITAPPDAKIERSGKIGPAETVMSLDVGVYIAA
                     GLSLVAALVHLWVMPEHLAEWWGYGAFFLAAALVQGFFAVAILRWPDQRLAFAGVWTN
                     LSIVLVYLLSRTSGLPVGPHAGVAEEAGVLDMTATASEVGVIILLVALLGGAYRKFTV
                     NALLLLGLAIWSLRLAGIIY"
     gene            complement(10472..12445)
                     /locus_tag="VFJ72_09035"
     CDS             complement(10472..12445)
                     /locus_tag="VFJ72_09035"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7479643.1"
                     /translation="MEMTLTRRDMLKMSLLGSAALMLPLERVARTQLQVERLAESQLP
                     KRFVAPFVRPPLAVPVHTDDTTDYYEMTMKQGYPEIIPGLKTEVWGYEGHTPGPTIRV
                     QRGRNVVVRHRNEIWNPLHEHTSVHLHGNASLPQYDGYANDTTNPGEFKDYHYPNFQD
                     ARTLWYHDHGVHVTGFNAYMGCAAFYITHDEREQSLTDPADGTYKDVGTAIPGGDYDV
                     PLVLRDAIFGTDGSLIFDDQGHSSLFGDVILVNGAPWPLMKVERRKYRFRILNASISR
                     GFNLKLSSGDPLTVIGHDGGLAPEPVAVNSMRIGMAERYEVVIDFSRYNPGETVDLNN
                     LGLPNIVDFASTNKVMRFEVVDSPVDTTPLPAELNPNTPVMNLTPAPGMKTRNFRFER
                     TLIGGQEMWTINGNVWDPNRVDANPGLGDTEIWEFENKSGGWFHPVHIHLVDFKILDR
                     NGRPPHPWERGPKDVVYIGQGETVRMIMKFAPQKGKYMIHCHNLVHEDHDMMTQFEVG
                     SDGPAWSSDPAKPTSQAGPLWEDNGGGGEQTTPDTTTPGNTTPENTTPNPPANTAPVI
                     SVLRPTPNMKTRDLTPTIAASIKDAQTKIAKSGVQLFIDGRKIGKFAYSQTTGKLVYT
                     CKTLRPGRHAVKVVATDPQGRSANKTWTFRVIR"
     gene            complement(12732..14363)
                     /locus_tag="VFJ72_09040"
     CDS             complement(12732..14363)
                     /locus_tag="VFJ72_09040"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7479644.1"
                     /translation="MELTRGEMLKLSLLGSAALMLPLERVARTRLKSADRLPKSRLPE
                     PFKVPFAVPPVLAPVCTDGGTDYYEIKMMAARLPMLPGLPPTEIWGYNGITPGPTIQV
                     MQGRKTVIRQMNLLPDRHPVLGYESTTSVHLHGSGSLPQYDGYANDTTSTGFFKDYHY
                     PNNQNARTLWYHDHGVHHTDENAYMGLAAQYHLHDQNEIGLPIPHGYWEESNPARPTG
                     GGRPGKYDVPLVIKDAIFATDGSLIFDDQGHSSLMGDVVLTNGRPWPVMKVERRKYRF
                     RILNASVSRGYDLALSSGDPMTVIGHDGGLAPAPVDVGHFRVGMAERYEVVIDFAKYS
                     VGQKVVLENRGLPNNADFKSTRNIMRFDVVGEATDPSGNDVPEVLNPGGFSHDPMKLQ
                     ESQAVDVHKLRLQRKNGQWTIDGKIWDPNRVDHRCHLDEIDIWELTNKSGGWFHPLHI
                     HLIDFRILDRNGRPPFPWERGPKDVAYIGEGETVRVLGQFGPNEGKYMVHCHNLTHED
                     HDMMTQFEVGAGGFDPIESARPKPISEAAPLCGTV"
     gene            complement(14779..17523)
                     /locus_tag="VFJ72_09045"
     CDS             complement(14779..17523)
                     /locus_tag="VFJ72_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479645.1"
                     /translation="MLARSTEARGSRWLALRDTLPWPARYGSAVVLAGLALALKLLLD
                     PLLRNESPFLLFFTAIVLGALVGGLGAGLVATALSALASDFFLLTPYHSLWIDDPGRL
                     LALGIFVAEGVLICAIVEAMYSARRRAEAALDSQKENQVALRFLAEASEALSSSLDYH
                     ATLASVARLSVPRLADWCAVDMIEEDGSLERLAVIHEDPEKIALALEMQERYPPDPDA
                     PYGLPQVLRTGEPELVPEITEALIEESVSDEEQRVLVRELGLASYMVVPLVARETPLG
                     AITFVYAESDRHYEQSDLELAVDLARRAALAVDNARLYREAQQEISGRKRIEEELRKS
                     GEQLQSVLDNSPAVVFIKDLESRYRLVNRRFEELFHVSREQVVGLSDHDIFTGEIADQ
                     LCANDREVLRRGVSLDLEEVVPQDDGMHTYASIKFPLRGPGGEPYAICGISTDITERK
                     RAEEALRQSEERYRVVTETASDAIVMIDEKNRVLFTNSAVENVFGYTSEELIGQQLTM
                     LMPPRLRSVHEASVKRYLQTGRRRLNWRSVQITGLHKSGEEIPLEISFGEFVREDKRF
                     FTGFIRDVTERKRAEEALQQSEERYRAVVEQITEGIYLLDGASRRVLETNPALRRMLG
                     YTEEEMRGMEVYDLLAHDREDVDANLARTLSEGHRFVGERRYLRKDGSVVEVEVGVST
                     ILYEGREVVCVTVHDITDRKRTEQALQDIREAERNRIARDLHDGVLQDLSYTAQALQV
                     ARVKSEGTHLESELEEQIETILRSARGLREAIYDLRLQSYRDQNFEGLLESLLELSRR
                     RAPDIRVEVEVQDGFSDLLARSGGMELLRIVQEALTNVRRHSGAGVVRIKLGYSEREI
                     WTEVSDDGWGLDTDSPPGTGIIGMRERAFGLGGRLEITSETGSGTTVRFEAPLSNLSD
                     "
     gene            complement(17776..18114)
                     /locus_tag="VFJ72_09050"
     CDS             complement(17776..18114)
                     /locus_tag="VFJ72_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479646.1"
                     /translation="MNVKRILISSDLRSYREAVAEAFRQLRPDCDVFEAESGDLNREV
                     LRLHPDLVVCSQATSLVRERVRNWVELYPDCEPYSVVSLEGERSTVADMQLSDLLSVV
                     DRTEHHAPLS"
     gene            complement(18186..18536)
                     /locus_tag="VFJ72_09055"
     CDS             complement(18186..18536)
                     /locus_tag="VFJ72_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479647.1"
                     /translation="MAHEPSRIRLLMANEPRAYREVIAEAMHELRPALVIMTVEPEDM
                     DGSVASFGPDIVICSAVTDLVESEVLAWVELYPEYGPQSVISVKGEVSKVNEIQLADL
                     LCVVDRAERLLQKG"
     gene            complement(18734..19423)
                     /locus_tag="VFJ72_09060"
     CDS             complement(18734..19423)
                     /locus_tag="VFJ72_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479648.1"
                     /translation="MIIDKSVDTGTTRIMLVEDHASFRQALAFMFDREQEFEVVAQAG
                     SLAEARKIVGSVDKRPDIAVVDLGLPDGDGFELIDEFSSASPQISTLVLSASLEPARF
                     ARAVEEGAAGVLHKAAAISDIVESVRRMKRGEALLSPTETIEMLRMVSRKRQEEYEVQ
                     KAVEKLTRREKEVLQSLAEGLDSKEIAEKLSITIETERTHMVNILNKLGVHSRLQALV
                     FAARNGVVEIR"
     gene            complement(19614..>23757)
                     /locus_tag="VFJ72_09065"
     CDS             complement(19614..>23757)
                     /locus_tag="VFJ72_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479649.1"
                     /translation="ESSVGALQVVRSGQSELYSEIPDEMLIAAARDEEHLKLIREIGF
                     TAAIMVPLVARGRTLGVISLVSAESGRRYEAADLALAEELARRAALAVDNAGLFEDAQ
                     REIAEREIAEQEMRNSRDQLDVILRGVTDGITAQDPTGHLIYANDAAARIAGYPSAQA
                     LVDAPLEEVLSRFEILDESGEPLPLSALPGRQALRGEPVQERTLCFYELATGEERWAV
                     VGATPIFDERGNVRLVVNIFRDVTEQRRAQEALQISEARFRTMIEQSPLSIQILTPHG
                     RTLQVNRAWEELFGTDLEGLGDYNLLEDRQLVEQGVMPQILKGFAGEPVRIPPIAYNR
                     YESIPELNQQDEPVLWTQAYVYPVKDSAGKVREVVLMHEDVSERRRAEEELIWLASFP
                     MLNPNPIVETTLDGEPTYLNPAAEAQFPNVRTLGSRHPALADLREAYERIWASGGRPI
                     TREVRVGERFYQQTISRVPESDLLRIYTTDTTDRRRAEDAVRKSEERFRSLVQNASDI
                     IVVLDAGGEILYESPAIERVLAYTPEERIGTNAFDYIHPEDQERVRDSFAKYIEDSEV
                     HAAVEYRVRARDGSWHYFEAVGTNLLDDPAVGGIVVNTRDVTERRVAEETLRESEERF
                     RATFDQAAVGVAIVGLDGNWLRVNDKLLGIVGYTSEEMRGLTFQDITYPGDLEDDLRG
                     MRRMLEGEISTYTREKRYFKKDGSIVWINLTVSLVRDNSGEPGYFIAVIEDIGQRKEA
                     EVALRISEEKYRTLFETMTQGVVYQDAGGRIISANSAAERILGMTLEQMQGKTSTDPR
                     WRAIHEDGSDYPGEEHPIVLALKTGREVRNDVIGVFVPETGEYRWVNVSAVPQFREDE
                     DTPYQAYAVFEDITERRRAEEQIRLRAREQEAVADIGRRALARGDLQTLMDYAVSRIA
                     EVLDLDCCGILELLPDSGSMLLRSGVGWEEGRVGEATVSADPGSQGGYTLALDEPVIV
                     EDLRAETRFDGSRLLRDHGVVSGMSVIIRRRERPFGLLGAYVREQRAFDDDDVNFLRV
                     IANVLATAIERRSVETALIQSEELYRTVVEQAAENIFVVDVETKEVLEANAALGRSLG
                     YTEEEIRSMTLYDIVDHERESIDERVERILALGRDFLGERVYRRKDGTSVDVEVSASA
                     IPYRGRNAMCIVAHDITERKRIEAAMREVREAERNRIARDLHDDILQDMVYALQEIQI
                     LQVTSEDGGAPALEDTAAALRRSVEGLRVAVFELRLSETLERSLLASMQALLELNRRM
                     VRNRYTIDMSISEDFPQELPERKGKELLRIIQEALSNARRHASPNHVQIELGLDGETA
                     WAEVTDDGAGFTLTDSGGGVGMSSMRQRALELGGEVRVQSEPGHGARMRFEVPLERLT
                     AE"
BASE COUNT         3799 a         7728 c         7712 g         4518 t
ORIGIN      
        1 actgtgcggt agcgagcgtg gtgagccgat gacatgagaa gtccatcgga gtcgcagttc
       61 agcggcaaag gtatggacga ggcctggcta cccgatgacg cgcagttgaa cgaggaggaa
      121 aggcgagatg acgcgagcgg ctctcctgac cacggtcatg atgttggcga tcttcctatc
      181 atcgggggtc gcgtgggcgg cgatgatcag gtgcgatggc ggagtctgcc ggggtacgaa
      241 cagcggcgac cagatgaacg gttcagcgcg ctccgacagg atgtacggcc tggccggtat
      301 cgacgtaatg aacggtaacc gcgacaacga cttgatgtac ggtggaggag ccgccgacag
      361 gatgttcggc ggttacggca acgaccgaat ctacggccag tacctaaatg acatgataga
      421 cggcggtccg gcatcggaca gaatatttgg cggctctggc agagacacgc tttacggcgg
      481 caccggcaac gactttattt ttgccgccga caaccagttc gactcaattg attgcggagg
      541 cggcgcagac gttgcggtca tagacttcga agaagaccag atgagcctca acgtccccct
      601 gatcgactac atcaccctga cgagatgcga aaccatcagg tacggctaac gtctttgctt
      661 cgtggaacaa caaaggagag ctttgtccca gataacggag ccggtaccgg ctccgttatc
      721 taacgcttga ccacgaacct tgccgtgttg gtaacgccct ggtttggctg gctaccgaag
      781 agagcggtca cgtaatatat gccgggcgcg agcggtctgt agcgcgtgct gaatcgagag
      841 ctgttgagca gcgtgaactt acggctcacc accgcgccat tatgcttgat gatgagctgg
      901 acgttcccgg tgtgagccgg caagaccata ccgtagatgg tcacgaattt tttgaccttt
      961 acatacttcg cggaggtgct cacggagatg atcatattta cgttcacgtg catctgggag
     1021 ctagtggaag actgtagctc atcctcttca cttacgaaca gcgcccgata gtcggtactc
     1081 ttatctactc tgactcccgt cagggagaag cttccgtcta cacccgtggt tacgcggccg
     1141 tttggcacgg gactccagga agtagcgtcc tccggcctca tttctagaac gaccgtcctg
     1201 ccagcgagcg cgtccgtacc cgaagctagc ttgccggaca ggttggtggt ccctccattg
     1261 taggtcaggg tcgagacgct cgacgacaac gtgatctcgg tcggtttggg agcaacggcg
     1321 tctgcccgtc ctgccccgga ctcgccaccg gctgagtcca ccgcggtgaa ccggtagtat
     1381 gaccgggttc ccttcggcgc atctgaatcc atgtaggaag gtgcggagag aaggttggcg
     1441 ttgagcttcg tatatgttcc gtcaggagag ccgctccggt agacgttgta gcccgcgagg
     1501 tctacctccg ggctcacgtc ccaggagaga ttgacgccgc cggcggagcc caccgccttc
     1561 aggccggatg gcggcgccgg agccaggtcc agggtggtcc cgtagatatc gtagacgccg
     1621 aggtccactc cgtcgcgctg cgacgtccag accactctgt ctccgtccac ggcgggatcg
     1681 tcctgcggac cggaggccgt gctcacggcg aactcatctc cgttcgagat atcctcgccg
     1741 tagatgtccg tgctgccgac gccccggtcg tcggcccaga ctaccacgcg gccgctgacc
     1801 tccggcgccc actggtcgcc ggctccctcg gtcaggcggc gctctccgcc gcccgccagg
     1861 tcgtaggcgt agatgtccag gtctccggcc gttcgctcgt cgcgccacac tacggtgtcg
     1921 ccgctgatat ccggggcctg ccgccagccg ccgctcgtat tgagttgctt ctgggcgccg
     1981 ctgaagaggt ccttgaagat gatgtccgcc tcgccggcgc tggagatctg ctgccagacc
     2041 acgcgctcgc cgctcacggc ggggctgcgc tggtccccgg ctcccgtggc gatgtccgac
     2101 accactcccg atgggagcgt cctgccgcgg acgtcccagt cgccgccgct gtcgtcctcc
     2161 cagaccacga agtctccgct aatgtccggg ttacgctggt cgccggctcc ggcggcgatc
     2221 tcgaacggct gcccgccgga ggagagggtc gagaggtcga ggccgtagat gtcccagttg
     2281 ccgctgctgt tgtcctgcca gacgacgaag ctgccgctga tggaagggtt ggcctgggta
     2341 gccaggtcgt tggtaacctg cgtctcgccg ccgccgggcg agaggtcccg gatgtagata
     2401 tccccgccgc ccaggccccg gtcatcagcc cagaccgcga gatcgccgct tatggccggt
     2461 gcgcgcttgc tggcgccgtc ggcggcgacg ggagattcgg gggcgacgac ggtagcgctc
     2521 gcggacggcg cacctatcag gagagaagcg agcagggccg cgagcagaaa tagccaggcc
     2581 cgcgctcccg gcagcggtga gaaacggtgc tccgataccg aacgtaccgg ctgaactatt
     2641 actgaacctc tcactggctt tcctctctcg ctaaaaactc gaagacgatc cagttgaata
     2701 atggtccagc aggcgcgtcc cgccatcgca aatatgagcg atctttcact agctcagttg
     2761 agctagctgc acggcgcgag gggggatcgg acccgctcat aaagagagtc ccgcccgaaa
     2821 acggacggga ctccagagag tcttcggtct tcgggtgtta cttgatcacc ttgaagctcc
     2881 gtaccgggct cgtgccgccg aggtggtccg cctctccggc gaagctcacg ctgaccgtat
     2941 agagacccgc ccgcggcggc ttgtatgaaa agctgtagcg ggagctcttt agggagaggc
     3001 tcttgttggc gagtaccggt gctccgttac gcttgatggt cagcttgacc accccggcat
     3061 gggccggagc gacggaaccg gagatcaccc ggctgtttcc aagcttgagg ttggtcgccg
     3121 ccacgttgag accgatcatc gccttcacct ttacgaccac catagcttcc gtggccttca
     3181 gcccggcgtc cggctgtcct gcgaagctga cccggtaggt cgtgttcttc agcggcttga
     3241 cacccgcgaa ggtgtacgag cctccggctc cggtctgtgc ggtcgcgccg gccacggcgc
     3301 tgaaggcggc cgtcccggcg ggcttctgct ccaggaccac ggtcttgccc gggagcgccg
     3361 cgccgtcgga ggtgagcgcg ccggagatgg cggtcgtccc tccgtagttc acgatggcgg
     3421 cgctcgcatt cgcggttatg gcggagccgg tcatcacgat cacctgcttg ctggcgctca
     3481 cagagggctg aagcccggtc gtcggttgac cggcgaagct cgcccggtac tcggtggtct
     3541 tcgcgggctt gacgtccgcg aggctgaagt tgccgtcggc gtccgtcttc aggctgccgt
     3601 tcgccacggc ggtgaacgcg gtctctccgg cgggcttctg ctccagggtc acggacctct
     3661 tagccagcgg gccgttggcg gtcgagagcg tgccggagag tgtcgtcgcc tgtccaggtt
     3721 ccaggctggc gggcgcggcg ttgatgttcg agctggtcgc gatgccggca agatccaggg
     3781 gcatcagcgg gtgtccgccg gcgtaggggt catctacgtc gccgatggcg ctcgggttca
     3841 gcgcggcggc cggggatggc tgaccgtaga cccgcatgat gccccagagc ccgccggaga
     3901 ggtgatggaa gaagaccccg cagccgtagc ggtagtcgcc gggtgtgccg ctgggaccgc
     3961 caacgctcgg cagatctatg ttcagggcgc ggcccacgct catgccgccc gcggtcccga
     4021 tcacggtcga gcccggatcg tcgggctggg cgcgccagga gtggccggag agctggaagg
     4081 agtgctcgcg gggcttgtcc tcgccctgca ggacccgcat ccgaacccgg tcgccggcgt
     4141 aggccctgaa gatgggagta tccgggtcgc cgtgcgcgct ggagctgaac acgttcgcca
     4201 tgcccgctcc gtccgggctg tcgttcgcca tcgggatgta gccgagacga tggtcgaagg
     4261 gcgcgttgcc atagttgaag cccttctcgc cctggtcctc gaagtccagc gctcccgcca
     4321 gaggctccgc cggcgtgggc ggatggtccg ggcgatccgg gatggggact ccggaggagt
     4381 cgcgcaggtt caggccgtcc tggaagaagg tcgtgaactc gcggaagtcg ttgtccgggc
     4441 cggggatgcg gatgtcggcg gagacgccgc tctttatctc gtcgccggtc ttcgggtcat
     4501 ggtaggtggc gcccttgggc tctatgttga gcccggcgaa gagaccgtgg tgccggtgtc
     4561 cgcgcgcgtc gccgaaatcg gtcaggttgg ttgctcccag ctcgccgggg gccacgtcgt
     4621 cggcgtacca ccggtagagc ttcctgtctc cgggagcgac cgtctcgtcc ttgttgtagc
     4681 cgacagccgt gccgtccgag ccgcgcacgt cgtactttac gagctgcggg tggagcgaga
     4741 cgcgcagccc agcaggtgtg ccggcgtcgg gctcggtggg cgccttggcg tctccgtcag
     4801 taccggcgtt gccgtgctgg agccacgcag gcgtcaggtg attggtgaga ccaacctcta
     4861 tgcagtcacc ctcgttggcc cgcaggacca gcggctccgg cttcttctca ccggacttga
     4921 tcgctgcttc atccgaagcg aggacgtaga tcagaccgta gggatcgttg tctccggcct
     4981 tgttgtaggt cagggacttc tgcatcgcga cgacctcgta tttcttgacc ggagctccgg
     5041 ccgggcacgg ggtgccgggg ttgttcgact ccggcggcgc ggatgatgtg gaggatatgg
     5101 ggctgctggt cggtactccg gcgaccgtcg cctcggtcgg cgaagcgtta tccggcaggg
     5161 gcttgagcga gggcacctgt ttaccgcggg cccgcaggat gccccacatc ccggagtaca
     5221 tatcgtccat accggtgctc ccgtagaggt agtctccggt gcaggtctga tccgtggagc
     5281 cgcaggtcat cttcgggacc tcgaagttga aggcctcgga cactccgagc tgccgggcat
     5341 tgataaacgg agactcggga tcgtccggct cttccttcca gcgcatgccg ttcacggtca
     5401 tcacgtgctg ctcctcctgg gagccctgga tcaggcgtat acgcaccggg tcttccgagt
     5461 aggcctggag gaccggggtc tgcgggtcgc cgtgcaccgt cgagctgaag acgtgggcgg
     5521 ggtcagtggg gttcccgttc ttgtccgtct ggcgcaacac gaacggcgca ttgcggtagt
     5581 tgacgctcat aactccgggg tcgtcgtccg ggaagtgctc cggcgcttcc ggagggttga
     5641 tcgtgtccgc cttgacgcgg ggatctccgt ccttcttcgt cagggatacg aagtcctgta
     5701 tccccagccc gaactcccgg aagtcgtcct tcgcgccggg accgatgaca tccacggccg
     5761 cgcccgccgc cgtcgccgtg caggcgttgg ggtcggcgcc gctgccgcag accggcccgt
     5821 tcgccgggtt gtttatgggc tgggagaagg cgcccgtctt ggggtcgcgg aagtccatgc
     5881 ccttcggttc cacgatcaga gccccgaaga gtccccggtt ctggtccacg gccgcgaagt
     5941 gatggtcgtg ggtgaagacg gtgcggagct cgtagtcggc gtaccagcgc tcgtggatgg
     6001 tctgccccgg ctcgtctccc gaccactgcg ggttccaggt cccgggcgcg gggtccggga
     6061 cccggcatcc gtcggtcctg ttgcaggacg gagcgcccgg accgccgttc atgacgtcct
     6121 tgttgaagtt catctgcttc tgcgtgaagg cggcctcctg gtagttccag ccgttcgagg
     6181 aaccgtcgga gccgagcacg tcgaacttca cgagatggat gtgctcgccg aacatgttgg
     6241 tctggttcag gttggtgaag gcgtcgtcgc cgagccagtt ggggagccgg ttcgtgaggt
     6301 tgtagttgat gcagtcgccg gcattggccc ggaagaagag cggctcaggc tggttcttgt
     6361 acgtcccgtc ggggttcttc tcaagcatct tggggacatc cttatccagc acgaggaacc
     6421 ttccctgcgt gtcatgccac ccggcctcgt tgtaggtaat gtcgcgctgc atcacggaga
     6481 cgttgtaggt gatctcgcgt gcccccgccg ggcaggggtc ggtgagcgga gcgccgggcc
     6541 tgggcgtcct gtcgccgtag tggagcttct ggacgttctt ctccaccgcg agcttgtcgg
     6601 cgacctcttt ctgagcgggg gtgaggttgg ggtcgttggg gtctaggttc gggtcgagcg
     6661 cctggcccgc gacgatgcgc gtcgcctgct gcaggtcctc gcgcttgacg gtcgtgggat
     6721 cgtcctggct ctcgtccacg tcgggggtgt tggggtcgtc cggccgggcc tcgcttatgc
     6781 cgtttatggg ctggggcgcc cgccaaccga atttgccggg gatgaagcgc gggtaaccgg
     6841 ggttgtccgg cgtggcggcg gggagcggcg tccggtccgg gagaggttgc aggttgcgga
     6901 cgttcacgcc gtcgggggtc gagttcttgc cgtcctccag cacgtcgtgg gtgcggaaga
     6961 gtgcccagaa accgtccgcg aagtgcgggt agaggtggca gtggaagatc gagtctccaa
     7021 tcgtcccctt cttgctgccg gacccgaaga gaaggtccgc cgtgtacgac tcgcccggtc
     7081 cgaaggtctg ggagtctatg gtggtcgagt cgggacggtt ggcgtcggtg atgttgtcca
     7141 tcggggcgtc gccggtgtcg gcgggctccg cgagccactg gtgggcgtgc atgtggaaga
     7201 cgtgagtctc cttcaccccc gcgagggcga accggatctt ggaggggtct ccgacgtagg
     7261 tgtgggttac gttggagatg tagcaggagg tcgagttaaa gcccggcgtg ccgattccac
     7321 agtcctcgac gctcgcgggg tcggtgttct ccggggtcgg cagccagggg ccggggccgc
     7381 tggcgagctt gatcatggcc gggtctccgg cgacccagga ggagagcgag gtctcctcgc
     7441 cgatgccgtc cggggcgcgg ttgcccggcg cttcgcggtg gcgctgctgg tccgaaccgt
     7501 agttgaaccc gaagccgatg ctgtgcggga tctcatcctg cgcgagctgc acgctctccc
     7561 ggaaggactt gccgccctgc gggtggatgt cggcgtccac gtacaggctg ccgctctggt
     7621 tcgcggttcc tgtgtagaga gggttcccgc tcaccggatc ggtccaggtg gaaccggcgg
     7681 gctcgacggc cagcgcgccg aagaggccgt gcgcgacgct gccgccgtcg ccttccgtgc
     7741 cggcggtcgc gccctggtcg cggatcatga acagcccttc ggcgttcggg gccttccaga
     7801 agtaggtgat cttgttctgc cccggcgtgt tgggaacggt ggtgtcgtcg ttgtagccaa
     7861 ccgcgctgcc gtcggaggtc tgcgcgtcgt aggcggcgcc gaagaccttc atggaggcgc
     7921 gggggttagt cgggagcccg tcgccgccgt ccgtcagctc gttggtgaag tccaccctta
     7981 cgcactcgcc ggcgttcgcc cggataacca gcgggcgcgg ccggagccgc cggttgcccg
     8041 cggggtcctg agacgggtcg gctgcgagcg gcttgctcca gttcttcacg gcggccttgt
     8101 cgccctgaag aacgtacatc tggccgttcg ggtcgctgtc gccccagcgg ttgtagggga
     8161 tgtccaggtt gatggcggcc acgtcgtagg aacggtccgc cgtcgccgcg ccgcaatccg
     8221 gagcggcctg ggcctccgcc ggagcggaga acgcgccgaa taacgaggga ccggcgacga
     8281 ccatcgcgcc cgccgctaga ccggccaccg ccgcgagcgc ccgcgggtgc cagaagctca
     8341 cggtccgcgg cgcctcataa ggcgtcccgc gcagcgcggc gatggagaac agcacgagga
     8401 atcccgcgag gagcgtaatg cccgcgtcgc ggcccgcctg cgcggcgaag gtcccggcgc
     8461 cggcgtgctg cgtcgccagg gtatccgccg ggacgctcgc gagcgcgtag gcgaaggcgg
     8521 cggtgagcgc ccaggtgaga cgagcggaga atccggtagc cgttccgaag aagaaagtag
     8581 agatccggcg agagaggtag gtggagaccc acagcgcgag cgccgcgagc ggcagcgcga
     8641 gcatggaggt ctggagccag tgtgtcaagg aaccgaggcc gaacgcctca tacgtcccga
     8701 agctcgcgga gtggagcgcc gcgccggcgg cgagcgcctg aacggcggcg agcgccaggt
     8761 agagccgcgc cctgcgggac tcgtccgcga gcgcgacgag cgtggcgacg agcgagatct
     8821 cgacggccac ggagaagagg tcgagcgccg cggccggatg cacctctccg gcgtgcggtc
     8881 ctataaacgg gattccggcg gtgcgggtga tgatgtagaa ggcgacgatc ccaaggttgc
     8941 cgaaagctcc ggcgaggaag aggcggcgcc ccggccagcg caggagcgcc acgccgaaga
     9001 gaccctggac gagagcggtg atcaggaaga acgccccgta gccccaccac tccgccaggt
     9061 gctccggcgc gacccacagg tggataccgg cggccgtcat ggagagcgtg gccgccgcgt
     9121 acaggcccag ggaacgtgcc agggctgtaa ggttcgtgtt cttcgcggag ataaaattcg
     9181 ccaccatcaa agctctcctc ttgagtcaga gtcctctacg ctaagaatga tggaacagcg
     9241 gagcccgccc gccatcgtgt agatgggcga tctttctcta gcccacttgg actagatctt
     9301 tctcacggac gctattaccg gagcagcccg gcctctccca ccggcgctac cttcatcacc
     9361 acccgcccga gcaggtcctc ctcgggcacg gccccgtagt cgcgggagtc gcgggagttg
     9421 gagcggttgt cgcccatcac gtagacgtgc ccgggaggca ccttgtccgg cccgtagaaa
     9481 gtcgaatcgg tcagctcgta gtccacgtag gactctttct tccgctcgcc gttcacgtac
     9541 agcacgccgt cccggacggc gaccgtgtcg cccggcaagc cgacgatccg ctttatcacg
     9601 aggtctccct ccgggtcgcg gaagacggcc acatcgccgc gctgcgggtc ggtgatgcgg
     9661 taggccagct tgagggccag cacgctgtcc cccaccttca gcgtaggcac catgctctcc
     9721 gaggggatca ggaagacctc cgccacgaac ggccgcacca ccccgaacat gaacgcgaag
     9781 gccaggaaca gtaccagcag caggtcgaac gcgctcctcc cgcgccgcgc cttaccagcc
     9841 tcgccgcgct ccacgatccg ggtctcctga ctcgctctct ttcgccggag aagcgtcaat
     9901 atattatccc ggcgagcctc agcgaccaga tggcgagccc cagaaggagc agggcgttga
     9961 ccgtaaactt ccggtacgcg ccgccgagga gagcgaccag caggataatg accccgactt
    10021 ccgaagccgt ggccgtcata tccagcaccc cggcctcctc cgccacaccg gcgtgcggcc
    10081 cgacgggcag gccggaggtg cgggagagca ggtaaaccag gactatcgag aggttcgtcc
    10141 agacacccgc gaaggccagt ctctggtccg gccagcgcag gatcgccacg gcgaagaagc
    10201 cctgaaccag agcggcggcg aggaagaacg ccccgtagcc ccaccactcc gccagatgct
    10261 ccggcatcac ccagagatgc acgagcgccg caaccagaga gagtccggcc gctatataga
    10321 cgccgacatc caggctcatg accgtctccg cggggccgat cttgccggac cgctcgatct
    10381 tcgcgtccgg cggagcggtt atccgggccg ttgtcgggtt cttgatctcc atgttcacag
    10441 tccgcgatcc cttctgcgag gtctctcctg gttagcgaat cacccggaac gtccaggtct
    10501 tgttcgcgct gcgtccctgc gggtcggtgg cgaccacctt caccgcgtgc cggccgggcc
    10561 ggagggtctt gcacgtgtac acgagcttcc ctgtggtctg gctgtaggcg aacttcccta
    10621 tcttgcgccc atcaataaag agctgcacgc cggacttggc gatcttcgtc tgggcatcct
    10681 taatgctcgc ggcgatagtc ggcgtaaggt cgcgagtctt catgttcggc gtgggccgca
    10741 ggacggagat cacgggcgcc gtgttggccg gaggattcgg cgtcgtgttc tcgggggtgg
    10801 tgtttcccgg cgtcgtggtg tccggcgtcg tctgctctcc gccgcccccg ttgtcttccc
    10861 acaacggacc ggcctgggag gtcggcttcg ccgggtcgga gctccacgcg ggaccgtcgc
    10921 tgccgacctc gaactgggtc atcatgtcgt ggtcctcgtg gacgaggttg tgacagtgga
    10981 tcatgtactt gcccttttgc ggggcgaact tcatgatcat gcgcaccgtc tcgccctgtc
    11041 cgatatacac gacgtccttc gggccgcgct cccacggatg cggcggcctg ccgttgcggt
    11101 cgaggatctt gaagtcgacg aggtggatgt gcaccgggtg gaaccatcca cccgacttgt
    11161 tctcgaactc ccagatctcg gtgtcgccga ggccggggtt ggcgtctacg cggttcgggt
    11221 cccatacgtt gccgttgatg gtccacatct cctggccacc gatcagggtc cgctcgaacc
    11281 tgaagttcct ggtcttcatg ccgggggccg gcgtgaggtt cattaccggc gtgttcgggt
    11341 tcagctccgc gggcaacggc gtggtgtcca ccggggagtc aacgacctcg aagcgcatga
    11401 ccttgttcgt cgaggcgaag tccacgatgt tcggcaggcc gaggttgttg aggtccaccg
    11461 tctctccggg gttgtacctg gagaagtcta tgaccacctc gtagcgctcg gccatcccga
    11521 tcctcatgct gtttaccgcc acgggctcgg gagccagtcc cccatcgtgg ccgatgacgg
    11581 tcagcggatc gccgctgctg agcttgaggt tgaagccgcg cgagatggag gcgttgagga
    11641 tacggaagcg gtacttcctg cgctcgacct tcatcagcgg ccacggcgcg ccgttgacga
    11701 ggatcacgtc cccgaagagg ctggagtggc cctggtcgtc gaagataagg gagccgtcgg
    11761 tcccgaagat ggcgtcgcgc agtacgagcg gcacgtcgta gtcgccgccg gggatggccg
    11821 tgccgacgtc cttgtaggtg ccgtccgccg ggtccgtcag gctctgctcg cgctcgtcgt
    11881 gggtgatgta gaaggccgcg cagcccatgt aggcgttgaa gccggtgacg tggacgccgt
    11941 ggtcgtgata ccagagggtg cgggcgtcct ggaagttcgg gtagtggtag tccttgaact
    12001 cgcccggatt ggtcgtgtcg ttggcgtagc cgtcgtactg cggcagcgaa gcgttcccgt
    12061 gcaggtggac ggaagtgtgc tcgtggaggg ggttccagat ctcgttcctg tgcctcacga
    12121 ctacgttgcg gcctctctgg acccggatcg tcggtccggg cgtgtgccct tcatatcccc
    12181 acacctcggt cttgaggccg gggatgatct cagggtagcc ctgtttcatg gtcatctcgt
    12241 agtagtcggt ggtgtcgtcc gtatggaccg gcaccgccag cggcggtctg acaaacggcg
    12301 ccacgaagcg cttggggagt tgactctcgg ccaggcgttc gacctgcaac tgcgtccggg
    12361 ccacccgctc cagggggagc attagcgcgg cgctgcccag caggctcatc ttcaacatat
    12421 cccggcgcgt gagcgtcatc tccatctatc tgcacctctc cttcgtctct ctcatctcgt
    12481 atgacagagc ggggcatcaa aacccaggcg ctcgatccag agacattaaa gagcgccgga
    12541 gggatgcgga catcgcgcaa acggacgaac ttttcctacc ggatttaggc tagaaagttc
    12601 tacttaaaca tatagacatg acgtatagat aaatgtatag acgccggact tctctagcgc
    12661 cgagcgtcct taccctagcg cgccgggcgg cgggcgcggc agggcctccg tctcgtgcgc
    12721 cgccctcttc atcagaccgt cccgcacagc ggcgcagcct cggagatcgg cttcggacgg
    12781 gccgactcga tggggtcgaa gccgccggcg ccgacctcga actgggtcat catgtcgtga
    12841 tcctcgtggg tgaggttgtg gcagtgcacc atgtacttgc cctcattggg gccgaactgc
    12901 ccgaggaccc ggaccgtctc gccctctccg atgtaggcga catccttcgg gccgcgctcc
    12961 cacgggaacg gcggtctgcc gttgcggtca aggatcctga agtctatgag gtggatgtgc
    13021 agcgggtgga accagccgcc gctcttgttc gtcagctccc agatgtctat ctcgtcgaga
    13081 tgacacctgt ggtctacccg attcgggtcc cagatcttgc cgtcaatcgt ccactgcccg
    13141 ttcttgcgct ggagcctgag cttatgcacg tccactgcct gggactcctg cagcttcatg
    13201 gggtcgtggg agaagccgcc ggggttgagg acctcgggga catcgttgcc ggacgggtcc
    13261 gtcgcctcgc cgaccacgtc gaagcgcatg atgttccggg tagacttgaa gtccgcgttg
    13321 ttcggcagcc cgcggttctc gagaacgacc ttctgcccga cgctatattt ggcgaagtct
    13381 atgaccacct cgtagcgttc ggccatcccg acgcggaagt ggcccacgtc cactggcgcg
    13441 ggggcgagtc ccccatcatg gccgatgacg gtcatgggat cgccggagct cagggcgagg
    13501 tcgtatcccc gcgagaccga ggcgttgagg atgcggaagc ggtacttgcg ccgttcgacc
    13561 ttcatcaccg gccagggacg gccgttggtc aggactacgt cgcccataag gctggagtgg
    13621 ccctggtcat cgaagatcag ggacccgtcc gtggcgaaga tggcgtcctt gatgaccagc
    13681 ggcacgtcat acttgccggg tctcccacct ccggtcggcc gggcggggtt cgactcctcc
    13741 cagtacccgt gaggtatggg caagccgatc tcgttctggt cgtgcaggtg gtactgggcg
    13801 gcgagtccca tgtaggcgtt ctcgtcggtg tggtgcactc cgtggtcatg ataccagagc
    13861 gtgcgggcgt tctggttgtt cgggtagtgg tagtccttga aaaagccggt ggaggtcgtg
    13921 tcgttggcgt agccgtcgta ctgcggcagg gagcccgacc cgtgcaggtg gacggaggtc
    13981 gtgctctcgt agccgaggac cggatgcctg tccgggagca ggttcatctg gcggatgacc
    14041 gtcttgcgtc cctgcatcac ctggatcgtc ggtccgggcg taatgccgtt gtagccccag
    14101 atctcggtcg gcggcaaccc cggcagcatc ggcaacctgg ccgccatcat ctttatctcg
    14161 tagtagtcgg tgccgccgtc cgtgcacacg ggcgccagga ccggcggtac ggcgaagggg
    14221 accttgaaag gctcgggcag ccggctcttc ggcagccggt cggcgctctt gagcctggtc
    14281 cgcgccaccc gctccagagg aagcatgagc gcggcgctgc ccagcaaact cagcttcagc
    14341 atctctccac gcgtgagctc catccccgca cctcctctat cgggctgcct gggaggagtc
    14401 ttcttccgta acccgagccc ggagcacgga acggcctgcg aaagtcagag cgacagcggc
    14461 tgtctcctgc gcgaaatcgg ccggggcatc gccgtgtgta agaaacaacg atcggtcata
    14521 atgcgcggca ggagtcaagg acaacctcct tgtcttcgta acgggcggtt cgtcggcgtc
    14581 ggcaaactct gtcgaaccgt ccggcttatg tcctccttat atcctcatca gtattcaagc
    14641 gcggcccggc aagcggcgca tcgcgcgtat gcgtgatttt tggctagtcc aaatccttta
    14701 gtaatattga gctagaaata tcaagacggc gggttgtgga gggtccggcc atgaggcggc
    14761 gagctcgagt atggtcagct aatccgagag gttggagagc ggggcctcga agcggacggt
    14821 cgtgccggag ccggtctcgc tcgtgatctc cagccgtccg ccgagcccga aggcccgctc
    14881 ccgcatgccg atgattcccg ttcccggcgg ggagtccgtg tccaggcccc agccgtcatc
    14941 ggagacctcg gtccagatct cgcgctccga gtatcccagt ttgatgcgaa cgactccggc
    15001 cccggagtgc cgccgcacgt tggtcagggc ttcctgcact attctcagaa gctccatccc
    15061 gccgctacgg gcgagcaggt ctgagaaacc gtcctgcacc tcgacctcga cccttatatc
    15121 cggcgcgcgc cggcggctca gctccaggag agactcaagc agaccctcga agttctggtc
    15181 ccggtagctc tgaaggcgga ggtcgtagat cgcctcccgg agcccccgcg cggagcgcag
    15241 gatcgtctct atctgctcct ccagttcaga ttccaggtgc gtgccctcgg acttcacccg
    15301 ggccacctgc agagcctgcg cggtgtagga gaggtcctgc aagacgccgt cgtggaggtc
    15361 gcgggcgata cggttccgct cggcctcccg gatatcctgc agggcctgct cggtccgttt
    15421 gcggtcggtg atgtcgtgta cggtgacgca gacgacctcg cggccttcgt agaggatcgt
    15481 gctcaccccc acctccacct cgacgaccga gccgtccttg cgcaggtaac gccgctctcc
    15541 gacgaaccta tgcccctccg agagcgtgcg cgccaggttg gcgtccacgt cctcgcggtc
    15601 gtgggccagc aggtcgtaga cctccatccc gcgcatctcc tcctcggtgt agccgagcat
    15661 ccggcgcagg gcggggttcg tctccaggac gcggcgcgac gcgccatcga gcaggtagat
    15721 gccctcggtg atctgctcta cgaccgcccg gtaacgctcc tcgctctgct ggagcgcctc
    15781 ctccgcccgc ttcctctcgg taacgtcccg gatgaagccg gtgaagaacc gcttgtcttc
    15841 ccgcacgaac tctccgaagg agatctccaa cgggatctcc tcgccgctct tgtgaagtcc
    15901 ggtgatctgc acggaccgcc agttaagacg ccgacggccg gtctggaggt agcgcttcac
    15961 cgaagcctcg tgaacgctcc gcaggcgcgg cggcatcagc atagtgagct gttgaccgat
    16021 cagctcctcg ctggtatacc cgaacacgtt ctcgacggcg ctgttcgtga acagcacccg
    16081 gttcttctca tcgatcatga caatggcgtc cgaagcggtc tcggtgacga cgcggtaacg
    16141 ctcctcgctc tgccggagcg cctcttccgc ccgcttgcgc tccgttatat cggtcgagat
    16201 gccgcagatc gcgtaaggct cgccaccggg accgcgcagg gggaacttga tcgaggcgta
    16261 ggtgtgcata ccgtcgtcct gggggacgac ctcctccaaa tccagagaga ccccccggcg
    16321 caacacttcc ctgtcgttgg cgcagagctg gtcggctatc tccccggtga agatgtcgtg
    16381 atcgctcagg cccaccacct gttccctgga tacatggaac aactcctcga accggcggtt
    16441 gaccagcctg taccggctct caaggtcctt gataaagacc acagccggag agttgtccag
    16501 aacggattgc aactgttccc cgctcttccg gagttcctcc tcgatgcgct tccgcccgga
    16561 gatctcctgc tgggcctccc tgtagagccg ggcattgtcc accgcgagcg ccgctctccg
    16621 ggcgagatcc acagccagtt ccaggtcgga ctgctcgtag tgcctgtcgg actccgcata
    16681 gacgaacgtt atggcgccga gcggcgtctc cctcgcgaca agcggcacga ccatatacga
    16741 cgcgagtccc aactcgcgca cgagaacgcg ttgctcttcg tcgctcacgc tctcttctat
    16801 cagggcttcg gtgatctccg ggaccagctc cggctcgcct gtgcggagga cctgcggcag
    16861 accgtaggga gcgtccgggt ccggcggata ccgctcctgc atctccagcg ccagcgcgat
    16921 cttctcgggg tcttcgtgga tgacggccag ccgttcgagg ctcccgtcct cctcgatcat
    16981 gtccacggcg caccagtccg ccagccgcgg caccgacagc cgcgcgacgc tcgcgagcgt
    17041 cgcatggtaa tcgagcgacg aggagagcgc ctcgctcgcc tcggccagga aacggagcgc
    17101 cacctgattc tccttctggc tgtcgagcgc ggcttcggct cgccgcctcg cggagtacat
    17161 ggcctcgact atggcacaga tgaggactcc ctcggccacg aagatcccca gcgccaggag
    17221 ccgtcccggg tcgtctatcc agagcgagtg gtacggcgtg agcaggaaga agtcgctggc
    17281 gagggccgag agcgcggtag ccacgaggcc ggccccgagc ccacccacca gcgcgcccag
    17341 gacgatagcg gtgaagaaca gcaggaacgg gctctcgttt cggagcagag gatcgagcag
    17401 cagcttgagc gccagcgcga gcccggcaag caccaccgcg ctcccatagc gcgcgggcca
    17461 cggcagcgta tccctcaacg ccagccagcg gctccctctc gcttccgtgc ttcgcgcgag
    17521 caaccgatct cccttcgcag gcgcccttcg caaacgcgcc tatcgcactc tcggcacggt
    17581 caatactggc atcgtctctg gtccggcgca tcacgcaaat gagtgatttt tcctggtctc
    17641 ggtatagcgg ccagcaggaa agagaacggt gagaatgaga tcttacgatt cacgagcttt
    17701 cgggagcgtc cgctgccgac cggaggcgag cggtcccgaa ggtcctgacc aggggtcctg
    17761 gcccggaggc gcggcctagc tcagaggggc gtggtgctcg gtccggtcca cgacggagag
    17821 caggtccgag agctgcatgt ccgcaaccgt cgagcgctct ccctcaagag acacgaccga
    17881 gtaaggctcg cagtccgggt agagctcgac ccagttgcgg actcgttctc tgacgagaga
    17941 ggtcgcctgg ctgcagacca ccaggtccgg gtgcaggcgg aggacctcgc ggttgaggtc
    18001 cccggactcg gcttcgaaca cgtcgcagtc cgggcggagt tgccggaaag cttcggccac
    18061 ggcctcgcgg taggagcgaa gatcgctgga tatcagtatg cgcttgacgt tcatgggctc
    18121 atgataagtc gcggaataac tttctccatc acgaaaactg gcgatctttt cctagctaga
    18181 ttgggctagc ccttctggag caggcgctcc gcacggtcca ccacgcagag cagatcggct
    18241 agttgaatct cattcacctt cgagacctcg cccttcacgc tgatcacgga ctgcggaccg
    18301 tattccgggt agagttcgac ccaggccagc acttcgctct ccaccaggtc cgtgacggcg
    18361 ctgcagatca ctatatccgg cccgaagctc gcgacggagc cgtccatatc ctccggctcc
    18421 acggtcatga tcacgagcgc gggcctcaac tcgtgcatgg cctcggcgat gacctcccgg
    18481 taggctcgcg gctcgttggc catgagcaga cgtatccggc tcggttcgtg cgccacgatg
    18541 tccgcaccga cggcgatggt cctgtcctaa ccttccggcc gcgcaggcca acggagatta
    18601 cggatacccg gcacagtacc tatctccacg agatatccca actcccccct ccccgcgcga
    18661 gacgcctctt gcgcccgctg ccctgccttt cgtcccgctc tcgtcccgcg aactcgcggg
    18721 aattccgctt agtttagcgt atctcgacga ctccgttccg ggccgcgaac acgagggcct
    18781 gcagccgcga gtggacgccg agcttgttca ggatgttcac catgtgggtg cgttccgtct
    18841 ctatcgtgat gctcagcttc tcggcgatct ccttgctgtc cagcccttcg gcaagggact
    18901 gcaagacttc cttctcgcgc cgggtgagct tctcgaccgc cttctgtacc tcgtactctt
    18961 cctggcgctt gcggctgacc atccgcagca tctcgatggt ctcggtcgga gagagcaggg
    19021 cttccccccg cttcatccgc cgcaccgact cgacgatgtc gcttatcgcg gctgccttgt
    19081 gcagcacgcc cgccgcgccc tcctcgaccg cccgcgcgaa ccgggccggt tccaggctgg
    19141 cgctgaggac cagggtcgat atctgaggac tcgcggaaga gaactcgtcg atcaactcga
    19201 agccgtcgcc gtcggggaga ccaaggtcca cgaccgctat gtcgggtctc ttatccacgc
    19261 tgccaacgat tttgcgcgcc tcggccaggg agcccgcctg agccacaacc tcaaactcct
    19321 gctccctgtc gaacatgaag gcgagcgcct gacggaaaga ggcgtggtcc tcgacgagca
    19381 tgatgcgcgt cgtccctgta tccacactct tgtctatgat catggccttt cttccagctt
    19441 gatatccata caccgattat gacgctattg cctctttact accataacgc aaacgaggta
    19501 gttttttcta gcccatatgg actagattcc tcttccatct tgatgttcca ttgcggacac
    19561 tctagcgtga actctctaag tgaacaccct gagcgaacgc tctgatcgga tgatcattcc
    19621 gctgtcaggc gctctagcgg gacttcgaaa cgcatcctgg cgccgtgtcc gggttcgctc
    19681 tgcaccctaa cctcgccccc cagttcaagg gcccgctggc gcatcgagga catgcccacg
    19741 ccacccccgg agtcggtgag cgtgaagccc gcgccgtcgt ccgtcacctc ggcccaggcg
    19801 gtctccccgt ccaggcccag ctctatctgg acgtggttcg gggaggcgtg gcggcgggcg
    19861 ttagagagag cttcctggat gatgcgcaga agttccttgc ccttgcgctc tggaagttcc
    19921 tgggggaagt cctctgatat ggacatgtct atggtgtatc gattgcggac catacgacgg
    19981 ttcagctcga ggagcgcctg catcgaggcg agtaatgagc gctccagcgt ctcgctcaac
    20041 cgcagctcga agacggcgac gcgcagcccc tcaacggagc gccggagagc ggcggccgta
    20101 tcttcgaggg ccggagcgcc gccgtcctcg gaggtgacct gaagtatctg gatctcctgc
    20161 agggcgtaga ccatatcctg caggatgtcg tcgtgcaggt cgcgggctat gcggttccgc
    20221 tccgcctccc ggacctcgcg catcgccgcc tcgatcctct tgcgctcggt gatgtcatgg
    20281 gcgacgatgc acatggcgtt cctgcctctg tacgggatcg cgctggcgct cacctccacg
    20341 tccaccgacg tgccgtcctt gcgccggtac acccgctccc cgaggaagtc gcggcccagg
    20401 gcgaggatgc gttcgactct ctcgtcaata ctctcccgct cgtggtcgac gatgtcgtag
    20461 agcgtcatac tccggatctc ctcctcggtg tagccgagcg agcggccgag ggcggcgttg
    20521 gcctccagga cctctttcgt ctccacgtca accacgaaga tgttctcggc cgcctgctcg
    20581 accacggtgc ggtatagctc ttcgctctgg atcagggccg tctcgacgct ccggcgctcg
    20641 atggccgtgg cgagcacgtt ggcgatgacc cggaggaagt tgacgtcgtc gtcgtcgaac
    20701 gcccgctgct ccctgacgta cgccccgagc aggccgaaag gccgctctct cctgcggatg
    20761 atcacgctca tcccgctgac cacgccgtga tcccgcagca accgcgaccc gtcgaagcgc
    20821 gtctcggcac gcaggtcctc cactattacc ggctcgtcga gcgccagcgt gtaaccaccc
    20881 tgagagccgg ggtccgcgct tacggtagct tctcccacac gcccttcctc ccagcccacc
    20941 ccggacctca agagcatact cccgctatcg gggagcaact ccaggatccc gcagcagtcc
    21001 aggtccagca cttccgcgat acgggagacc gcgtagtcca tgagggtctg gaggtctcct
    21061 ctggcgagcg cccgccggcc gatatccgcg acggcttcct gctcgcgcgc cctcaaccgg
    21121 atctgctctt cggcccgccg gcgctctgtt atgtcctcga agaccgcgta tgcctggtac
    21181 ggggtgtcct catcctccct gaactgcggc acggcgctca cgttgaccca gcggtactcg
    21241 cccgtctccg ggacgaagac accaatgacg tcgttcctca cctctctgcc ggtcttcaac
    21301 gccaggacta tcggatgctc ctctccgggg tagtccgatc catcctcgtg gatcgcgcgc
    21361 caccgcgggt ccgtcgaggt cttcccctgc atctgctcca gcgtcatccc gagtatcctc
    21421 tcggcggcgg agttcgcgga gattatcctg ccgccggcgt cctggtagac gacgccctgc
    21481 gtcatggtct cgaagagggt ccggtatttc tcctccgaga tccggagcgc gacctccgcc
    21541 tccttacgct ggccgatgtc ctctatgacg gcgatgaaat atccaggttc tcccgagtta
    21601 tcccgcacca gcgagacggt caggttgatc cagacgatgg atccgtcttt cttgaagtag
    21661 cgtttctccc tggtgtaggt agatatctcg ccctccagca tccgccgcat cccccggagg
    21721 tcgtcctcaa ggtcgccggg ataggttatg tcctggaagg tcaacccgcg catctcctcg
    21781 gaggtgtagc cgacgatacc gaggagctta tcgttcaccc tcagccagtt accgtcgagc
    21841 cccacgatag ccacgccgac ggcggcctga tcgaaggtcg cccggaacct ctcctcgctc
    21901 tcgcgcagcg tctcctcggc taccctccgc tcggtcacgt cgcgcgtgtt gacgacgatc
    21961 ccgccgacgg ccgggtcgtc cagcaggtta gtcccgaccg cctcgaagta atgccaggac
    22021 ccgtccctgg cgcggacccg gtactcgacc gccgcgtgga cctcgctatc ctcgatgtac
    22081 ttcgcgaagg agtcccgcac cctctcctgg tcttccgggt gtatatagtc gaaggcgttc
    22141 gtcccgatcc gctcctccgg cgtgtaggcg aggacgcgct ctatggccgg gctctcgtag
    22201 aggatctccc cgccggcgtc gagcacgacg atgatatccg aggcgttctg gaccagggag
    22261 cggaaccgct cctcgctctt ccggacagca tcctccgccc ggcggcgatc tgtcgtatcg
    22321 gtcgtatata tgcgcagtag atcgctctcc ggcacgcgcg agatagtctg ctggtagaac
    22381 ctctccccga cgcgcacctc ccgcgttatt ggccgtccgc cggaggccca gatcctctcg
    22441 tacgcttccc gaagatccgc cagagcggga tggcgggacc cgagcgtccg cacgttcggg
    22501 aactgcgctt ccgccgcggg gtttaggtag gtcggttcgc catcgagcgt cgtctccacg
    22561 atggggttgg ggttgagcat ggggaaggac gcgagccaga taagctcctc ctccgcccgc
    22621 cggcgctcgc taacgtcctc gtgcatcagg actacctcgc gcaccttccc ggccgagtcc
    22681 ttgaccgggt agacgtatgc ctgggtccag agcaccggtt cgtcctgctg gttgagttcg
    22741 gggatcgact catagcggtt gtacgctatc ggcgggatac ggacgggctc tcccgcgaac
    22801 cccttgagta tctggggcat gacgccctgc tccacgagct ggcggtcctc cagcaggttg
    22861 tagtctccaa gaccttccag gtccgtcccg aacagctcct cccatgcccg gttcacctgg
    22921 agcgtgcggc cgtggggcgt gaggatctgg atgctcaggg gcgactgctc gatcatggtg
    22981 cggaaacgcg cctcggagat ctgcaacgcc tcctgggccc gccgctgctc cgttacgtcg
    23041 cggaagatgt tgactaccag ccggacgttg ccccgctcgt cgaagatcgg cgtcgcgccg
    23101 accacggccc accgctcctc gcccgtcgcc agctcataga aacacaacgt cctctcctgg
    23161 accggctcac ccctgagcgc ctgccggccc ggaagcgcgg agaggggcag cggttcgccg
    23221 gactcgtcca ggatctcgaa ccggctcaac acctcttcca gcggagcgtc gacgagcgct
    23281 tgagctgatg gatagccagc gatccttgct gcggcatcgt tcgcgtagat gagatgacct
    23341 gttgggtcct gggcggtgat gccgtcggtg actccccgca ggatgacatc gagctggtcc
    23401 cgggagttcc gcatctcttg ctcggcgatc tcccgctcgg cgatctcccg ttgcgcatcc
    23461 tcgaagagcc ccgcgttgtc caccgccagg gctgcccggc gggcgagctc ctcggccagc
    23521 gccaggtccg ccgcctcgta ccggcggccc gactcggccg acaccagcga gatcaccccg
    23581 agcgtccgcc cccgcgcgac cagcggtacc atgatcgccg ccgtgaagcc gatctcgcgt
    23641 attagcttca gatgctcctc gtcccgcgcg gcggcgatca gcatctcatc ggggatctcg
    23701 gagtagagct ccgactggcc gctccgaacc acctgcaagg cccccaccga ggattcg
//
LOCUS       DASSMB010000152         8744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1556211, whole genome shotgun sequence.
ACCESSION   DASSMB010000152 DASSMB010000000
VERSION     DASSMB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8744
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556211"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VFJ72_09070"
     CDS             complement(<1..449)
                     /locus_tag="VFJ72_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479650.1"
                     /translation="MRKVMMLAAMLAMVLVAAAPAFAQATGGDVNVQYVDCSQVAAVA
                     AQQTQAGDATAASGDVGSAAAADIANELGISVEQVNGCLGGVGNETGDEDMGGGEDMS
                     SSSSSSAAAASSGGAASSGGAAASSGTLPDTGGASLITLGAGALLVAG"
     gene            complement(684..1211)
                     /locus_tag="VFJ72_09075"
     CDS             complement(684..1211)
                     /locus_tag="VFJ72_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7479651.1"
                     /translation="MRYLAVVFASFFLSAVTVAGVTLVEPEKVDAATVGVKGCTGTIV
                     KLTQAEKRMLDHHNKTRADRGLSRLCVHPALEKAARAHSQDMIRKDYFAHGNVGKRLH
                     KFGYRWRTYGENIAYGSGSSGSPDAIFKAWMHSSSHRSNILSGKYKEIGIGAATGTYK
                     GTKNVTMWTADFGSR"
     gene            complement(1303..2142)
                     /locus_tag="VFJ72_09080"
     CDS             complement(1303..2142)
                     /locus_tag="VFJ72_09080"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7479652.1"
                     /translation="MRTPVAAQVSPPITEVSVKELDTRLAEIARSHTGTYEAAVYEPE
                     SGKTFGINPDRPFMAASLAKLPVLIALYKAAARHELSLDEKISILPSDVQNYGTGVLN
                     GYPVGSTMTLRECASYLIKESDNTAWVMLERRLGRAKIEAELAGLGAQGTNYQAITTT
                     PRDVMLMLRAIADPGYTTPDLSSEMLSLMTGTAYEDRLPEPLPKGTRVAHKIGSYEDT
                     FSDAGVIFHKSPDGKENNYFIVVVGRNTTEDEARAAIREMSLATYQAFIDPLAADPAS
                     KRG"
     gene            complement(2405..3151)
                     /locus_tag="VFJ72_09085"
     CDS             complement(2405..3151)
                     /locus_tag="VFJ72_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479653.1"
                     /translation="MEERKIPETLEEAYRLRDSSDELTDRDFYQEQVYRLRREEYRSR
                     GIRTEISSRRRERQSAIEFSREMFFLEVSEITDGPRRGSGRDARVVQQEARRYATRLD
                     THAFLKQLASPHSGAVDLYRRNYLELLRMGKPPAEAYYLSRVIGVVGDEEFRRAVDKV
                     RDTGYPKGRIPYVVERAAIQIRDLPGGMILILLTFFASLFAFVWATNEWAGTASAGTI
                     LVATLSLIAAGALMLISAVFIVTFIRSINK"
     gene            complement(3240..3776)
                     /locus_tag="VFJ72_09090"
     CDS             complement(3240..3776)
                     /locus_tag="VFJ72_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479654.1"
                     /translation="MEKMSNQEPERRERSRERQARKRRGPDEFWENIGLDVESMSPRV
                     QAITGVVTGGLILLAAALVLTTQLWWLIFVFGWAVFPAFGVFARGVAGLVEARSELPP
                     RDSREKELLEALQRRGELTPAQAAMETSLSVRQADEMLENLASKGHLDVRVRGGGIFY
                     SLWDHEERADERRLEGGK"
     gene            3892..5241
                     /locus_tag="VFJ72_09095"
     CDS             3892..5241
                     /locus_tag="VFJ72_09095"
                     /inference="COORDINATES: protein motif:HMM:NF005034.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7479655.1"
                     /translation="MERDSRIESMKDALLEELNEFLRMPSISARENAGGEFRECAGWV
                     VEKLEDLGAEARLMETSGHPVVYAEIGEGDKTLLSYGHYDVQPPEPLELWESDPFEPT
                     VRDGSLFARGVADDKGDVLARIQALRFYIEEHGPPPFKLKFLIEGEEEIGSPNLANFV
                     RSNKDLLAADACLWEGSIKDEVGHPVIFCGTKGMAYVELRTKGASHDLHSMYGGIAPN
                     PVWRLVQALRTIKDENGEITLDGFEELIEPPSEQDLEAIKRIPFDDAEMRRSWGVEAL
                     DRNLTGQDALREMLLRPTANIAGIMSGYTGPGSKTIVPSEAFVKMDFRLVSGQSPEPV
                     VKLIREHLDRRGFDDIEVVDLHGVEAAKTPVDSPVVQTSVETWRDLGRDDAVVYPTIG
                     GSGPTSLFAMELGIPTVMTGNVANSGSRIHSPNESVRLDDFFEAVRYFNRFFERFAD"
     gene            5238..5822
                     /locus_tag="VFJ72_09100"
     CDS             5238..5822
                     /locus_tag="VFJ72_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479656.1"
                     /translation="MNEARRKGLDLPERVRRSLASAVAERGVQARVISTQGRDSMRPP
                     QGHIIVAGTQDEIDGIRGPVRIARQLEHYKTPYGPVVRLAFSVYPMRGDPLSAGTLLN
                     ISQVSGDAALTGLGRQKSVYIHFYEASGDDLTYAFSKELTNASEQRAEAKKLLKMARD
                     TYAETPEERRSFRWAVGIAEKQFELPVPLPDDEA"
     gene            5863..6993
                     /locus_tag="VFJ72_09105"
     CDS             5863..6993
                     /locus_tag="VFJ72_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479657.1"
                     /translation="MGSTSKDFVDKISEALSGAGRRGIHVTAGTCDLERQQFAPVQPD
                     EEDQAPPGRPHHLETPPGPRDIGSPDGTSRLCYFLDGVQSSREIGRVEMSPVVVTTVA
                     AAIVNRCDRKFSRMPLESPPVVVQAVILPRSAGDAQVGALWDLLLDAGFSELGSDEVP
                     SSPHLILDSAQYVPEADPSDYVGMREMARVRVRALRERLEGGMLREWELDDRTLEDSD
                     AWIAVDGQLRDIRESNRRAIGLIKSVARPEFVGKGVGMLLDLEPGMRTTSFVPDWQLR
                     RDPGEQRTSWYLRMWPPQRGADALGSLMRIEAPRDTDPEQVDEISRWVLAERAPLAKP
                     DPRWPAMIYPIHYVEKILKPLVQGSERAYSRLERQLAANGRN"
     gene            6994..>8744
                     /locus_tag="VFJ72_09110"
     CDS             6994..>8744
                     /locus_tag="VFJ72_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479658.1"
                     /translation="MLHPDYLDTDLGPIGRVVTSEDQPATAHEFYFWTAETEAAQNLD
                     IGHIVVAEAEDATAIAVLDDPRRYSDLQSFLDDFYAYDGDPSLEALSERVEILVFRAR
                     VLATKHRDERKKSKRPVRTGPVFFATSAAIEYALGTDEFAGHKIPMLLHENGNEKDGS
                     PQRTPLYVDGDYLLGPEAGHLNITGMSGLSTKTSHALFTISSTFQTVKDKKVAALMFN
                     VKGADLLFLDKPVEVDPQDDPDLAARYEKAGQRGLPREDREMYASLGLEIKPFENLRI
                     FAPLAFGKDRGERKVHAGDLRAKDLNTLRRAFGEDSCVYPIVWDLEDVLPHAGRIFDQ
                     NDMDDKFKGFVEYLRDRHVRTMSAFHAEVDEAFAYFEESESSYWNGHHQATVAKVRNR
                     FQVLPSKCAGLLVNGRVEYGDTPEVDGPFGDREMRVVDISHLSGVPQDLVVTKVINSV
                     WEMAEQGRLGVDKLVIFVDELNKYAPSGSRSSSLKDTLVDISARGRHLNLTLFGAQQF
                     RSKVDDEVVGNAATSLYGRIGDEELTNSSYRSFSQTTREELLQLEKGRLLLRHAHYAV
                     PVFGTFPRPMVLMGKQGT"
BASE COUNT         1698 a         2830 c         2710 g         1506 t
ORIGIN      
        1 ccggcaacca gcagagcgcc agcgccaagc gtgatcagcg aagcgccacc cgtgtcaggc
       61 agcgtgccgc tcgaagcagc cgcgccaccg gaagaagccg cgccaccaga gctggcagca
      121 gccgcgctgg aggagctgga ggagctcatg tcctcgccgc cacccatgtc ctcgtcgcca
      181 gtctcgttgc caacgccgcc gaggcagccg ttgacctgct caaccgagat gccaagctcg
      241 ttggctatgt cggcagcggc cgcgctgcca acgtcgcccg aagccgcagt ggcatcgcca
      301 gcctgggtct gctgggcagc tacagccgca acctgcgaac agtcaacata ctgaacgtta
      361 acgtcgccgc cggtcgcctg ggcgaaagcc ggagccgcgg caaccagcac cattgccagc
      421 atagcggcta gcatcattac cttcctcata cttacctccc tcattggtac accatcaaca
      481 accatgattt ttaacacgat ggacagcatc tgtaaaccct attctcaata aaacttaaca
      541 aatctacttt tgtttttctc gtaccttcgt gtatgggttg caaggtactg ccccgggcaa
      601 ccagcctgca tacagtaaag agagccccac cactgggggc tctctcctcg tagtaggatt
      661 ctgaggctgg ctctctacgg cttctagcga gaaccgaagt ccgcggtcca catggttacg
      721 ttctttgtcc ccttgtaagt ccctgtggca gcaccgatac cgatctcttt gtacttccca
      781 ctgaggatgt tcgaacgatg gctggagcta tgcatccagg ccttaaagat agcgtctgga
      841 gagcccgagg agccactacc atacgcgata ttctcgccat aggtccgcca gcgatatccg
      901 aacttgtgca ggcgcttacc gacgttgccg tgtgcgaagt agtccttacg gatcatgtcc
      961 tgtgagtgag cgcgagcggc cttctccagg gcgggatgta cgcagaggcg tgaaagcccg
     1021 cggtcggcac gggtcttgtt atggtggtcc aacattcgct tctccgcctg ggtcaacttg
     1081 acgatggtgc ccgtgcagcc cttgacgccc acggtagccg catcgacctt ctccggctct
     1141 acaagcgtaa cgccagcgac ggttacggca gacaggaaga acgatgcgaa aacaactgcc
     1201 agatatctca tcaactactc ctttcggtta caaactctcg cgtgtttaga ttacctaccc
     1261 cccaaactaa atctttagta acgcggaatg gctgtaatag ctctaaccac gcttactggc
     1321 tggatcggcg gccagcgggt ctatgaaagc ctgataggtc gcgagggaca tctcccggat
     1381 ggcggcgcgg gcctcgtcct cggtggtatt ccggccaaca acgactataa agtagttgtt
     1441 ctctttccca tccgggctct tgtggaagat cacaccggca tcgctaaagg tgtcctcgta
     1501 ggagccgatc ttgtgtgcga cgcgggttcc cttggggagg ggttcgggga gacggtcctc
     1561 gtaagcggtg ccggtcatca gggagagcat ctcggaggag agatccgggg tggtgtagcc
     1621 ggggtcggcg atggcccgca gcatgagcat gacgtcccgg ggggtcgtgg tgatggcctg
     1681 gtagttcgtg ccctgcgctc ccagaccggc cagctcggct tcgatcttcg ccctgcccaa
     1741 acggcgttcg agcatcaccc aggccgtatt gtcgctctct ttaatgaggt atgaggcgca
     1801 ctcgcgcagg gtcatggtgg agcctaccgg gtagccgttg aggacgccgg taccatagtt
     1861 ctgcacgtcg gaggggagga tcgagatctt ctcgtcgagg cttagctcgt ggcgggccgc
     1921 ggccttgtag agggcgatca atacaggcag cttcgccagg ctcgccgcca tgaagggccg
     1981 gtcagggttg atgccgaagg tttttccgga ttcgggttcg tagacggccg cctcgtaagt
     2041 cccggtatga gaacgggcta tctcggcgag ccgcgtgtcg agttccttta ccgagacctc
     2101 cgtaatcgga ggggatacct gcgcggccac cggggtccgc acccggtcaa ccttcacggg
     2161 accggcgttt gagaccacgc gcgagaagag gaagaagacg ccaagaaggg cgagaaatat
     2221 gagaaaagct atccagcgcc gtctccggta gaccgagcgc cgatccaggc gggcttgcct
     2281 gcgacgctgt acccgcgtgc gtttctcgac ttcaacgggc gtcgcctttc tgggcgccgg
     2341 tgcgtagata tctcccccat ttaaacgtct cgctccggcg cacgagcgcc ggacctattt
     2401 ggacctattt atttatcgac ctgataaagg taacgataaa taccgccgag atcaacatca
     2461 aagctccggc ggcgatgagg ctgagggtgg cgaccaggat cgtccccgcg ctcgcggtgc
     2521 ccgcccattc gttagtggcc cacacgaaag cgaagagcga ggcaaagaag gtgagcagga
     2581 tcaggatcat gccgccgggc aggtcccgga tctggatcgc cgcccgctcc accacgtatg
     2641 ggattctccc tttcggatat ccggtgtccc gcaccttatc cactgcgcgc cggaactcct
     2701 cgtcgcccac gacccctatt acccgtgaca ggtagtaggc ctcggccggc ggtttgccca
     2761 tacgcagtaa ctccaggtaa ttgcggcggt agagatccac cgccccggag tgcggagagg
     2821 cgagctgctt gaggaaagcg tgagtatcca gacgggtggc gtaacggcgg gcctcctgct
     2881 gaacgacgcg cgcatcccgg ccggagccgc gcctggggcc gtccgtgatc tcggagacct
     2941 ccagaaagaa catctcgcgg gagaactcga tggcgctctg gcgttcgcgg cggcgggagg
     3001 agatctccgt ccgtatcccc cgcgaacggt actcctcgcg ccggagacga tagacctgct
     3061 cctggtagaa gtcgcgatcc gtcaactcgt cggacgagtc tctcagcctg taggcctctt
     3121 cgagcgtctc tgggatcttt ctttcttcca taaaaccttg tctaccccgc gctcaagctc
     3181 ttacgcgcca agcccttcct ggaccccgaa tgataccatg agagcgacaa atcaaatacc
     3241 tacttaccac cctccaaccg cctctcgtcg gctctttctt cgtgatccca gagactgtag
     3301 aagataccgc ctccccgaac ccgcacgtcg aggtgtcctt tggaagccag gttctccagc
     3361 atctcgtcgg cctgcctcac ggagagcgaa gtctccatcg ccgcctgagc cggggtcaac
     3421 tcgccgcgcc gctgcaacgc ttcgagcaac tccttctccc tggaatcccg tggcggcaat
     3481 tccgagcgag cctccacaag gcccgctacc ccccgcgcaa agacgccgaa cgcggggaaa
     3541 accgcccacc cgaagacaaa gatcaaccac caaagttgcg tagtcagaac gagagccgca
     3601 gcaagaagga tcagaccgcc ggtcacgacg cccgtgatag cctggaccct gggactcatg
     3661 ctctccacgt ccagcccgat attctcccag aactcgtccg ggccacggcg ctttcgagct
     3721 tgccgctcgc gacttctctc gcggcgctcc ggctcctggt tggacatctt ctccacggcg
     3781 ctacctccgg cctcaatata acacggctcg cccgcgctcc gatgccttcc aaatctggcg
     3841 agaacgcgcg aaagtgctaa cctctccgca gtcgaaacgg agggtttgag gatggaacgg
     3901 gactcgcgca tagagagtat gaaagacgcg cttcttgagg aacttaacga gttcctgagg
     3961 atgccttcta tctcggcaag agagaacgcg gggggcgagt tccgcgagtg cgccgggtgg
     4021 gtcgtagaga agctggagga tctcggggcc gaggcgcgcc tgatggagac ctccggacat
     4081 ccggtcgtgt acgcggagat aggcgaggga gacaagactc tcctctccta cggacactac
     4141 gacgtgcagc cgccggagcc gctggagctg tgggagagtg atccgttcga gcccacggtc
     4201 cgggacggaa gcctcttcgc gcgcggggtc gcagacgaca agggcgacgt gctcgcccgc
     4261 atccaggcac tgcggttcta tatagaggag cacggcccgc cgccgttcaa gctgaagttt
     4321 ctgatcgagg gcgaggaaga gatcggcagc ccgaacctgg cgaacttcgt aagatccaac
     4381 aaagacctgc tcgccgccga cgcctgcctg tgggagggct cgataaaaga cgaggtcggg
     4441 cacccggtca tcttctgcgg cacgaagggg atggcctacg tcgagcttcg cacgaagggc
     4501 gcatcccacg acctgcacag catgtacggc ggcatcgcgc ccaacccggt gtggcgtctg
     4561 gtgcaggcgc tgaggacgat caaggacgag aacggcgaga taacgctgga cgggttcgag
     4621 gagcttatag agcctccctc cgagcaagac ctggaagcga taaagcgcat cccgttcgat
     4681 gacgcggaga tgcgccgctc ctggggcgta gaagctctgg accggaacct taccggacag
     4741 gacgccctgc gcgagatgct cctgcgcccg acggcgaata tcgctggcat catgtccggc
     4801 tacacgggac cgggctcgaa gaccatcgtc ccatcggagg cgttcgtgaa gatggacttc
     4861 cggctcgtct cgggccagag cccggagccg gtggtgaagc taatacgaga gcacctcgac
     4921 cgccgcggct tcgacgacat cgaggtcgtt gatttgcacg gcgtggaggc ggcgaagacg
     4981 cccgttgact cgcccgtcgt ccagacgtcg gtcgagacct ggagagacct cggccgcgac
     5041 gatgccgtgg tctacccgac catcggcggc tccggcccga cctcgctctt cgccatggag
     5101 ctcggcatcc cgaccgtgat gaccggcaac gtggcgaact ccgggagccg catccactcc
     5161 cccaacgagt ctgttcgcct cgacgacttc ttcgaggccg tccggtactt caaccgcttt
     5221 ttcgaaaggt ttgcagattg aacgaagccc ggcgcaaggg gttggacctg ccggagcgcg
     5281 tccggcggag cctcgcctcg gcggtcgcgg agcgcggggt gcaggcgcgg gtgatctcta
     5341 ctcagggccg cgattcgatg cgtccgccgc aaggccacat catcgtcgcc ggtacacagg
     5401 acgagataga cggcatccgc ggtccggtcc gcatcgcccg ccagctagag cactacaaga
     5461 ccccctacgg ccccgtcgtc cgcctcgcct tctccgtcta tcccatgaga ggagatccac
     5521 tctccgccgg gacgctcctg aatataagcc aggtctccgg cgacgccgcc ctgaccggac
     5581 tgggccggca gaagagcgtc tacatacact tctatgaggc atccggcgac gacctgacct
     5641 acgctttctc caaggagttg accaacgcct ccgaacagcg cgccgaggcg aagaagttgc
     5701 tcaagatggc ccgcgacaca tatgcggaga ccccggagga acggcggagc ttccggtggg
     5761 cggtcgggat cgcggagaaa cagttcgagt tgcctgtacc tctacctgac gatgaggcat
     5821 agtcttctct aacggagccg cgtatacttt ccggcaatgc tgatggggag tacgtcgaaa
     5881 gattttgtcg acaagataag cgaggctctg agcggcgccg gtcgcagagg catccatgta
     5941 accgccggga cttgcgacct ggagcggcaa cagttcgcgc ccgtgcagcc ggacgaagaa
     6001 gatcaagccc caccgggacg cccgcaccac ctggagaccc ctccgggacc gcgcgatatc
     6061 ggcagtcctg acggcacttc gcggctgtgt tacttcctcg atggggtgca gagctcgcgg
     6121 gagatcgggc gcgtggagat gtctcccgtc gtcgtcacca ccgtcgccgc cgccatcgtc
     6181 aaccgctgcg accgcaagtt ctcgcgcatg ccgctggaga gcccgccggt cgtggtgcag
     6241 gccgtgatcc tgccgcgcag cgccggcgac gcccaggtcg gggcgctctg ggacctgctg
     6301 ctcgacgccg ggttctcgga gcttgggtcg gacgaggtcc cctcttcccc gcacctgatc
     6361 ctggactccg cgcagtacgt gcccgaagcg gacccctccg actatgtcgg gatgcgggag
     6421 atggcccgtg tgcgcgtccg ggcgctccgg gagcggctgg agggcggaat gctccgggag
     6481 tgggagctgg atgaccggac gcttgaggac tcggacgcct ggatcgccgt tgacggacag
     6541 ctcagggaca tccgggagtc gaaccgccgc gccatcggcc tcataaagag cgtcgcccgc
     6601 cccgagttcg tcggcaaggg cgtaggcatg ctactcgacc tggagccggg gatgcggacg
     6661 acgagcttcg tcccggactg gcaactccgg cgcgacccgg gcgagcagcg gacgtcctgg
     6721 tatttgagga tgtggcctcc gcagcggggc gcggacgcgc tgggatcgct aatgaggata
     6781 gaggcgccga gggacacgga cccggagcag gtagatgaga tcagccgctg ggtcctcgcc
     6841 gagcgagcgc cgctggcgaa gccggacccg aggtggccgg cgatgatcta cccgatccac
     6901 tacgtcgaga agatcctgaa gccgctggtg caaggctcgg aacgagctta ctcgcggctg
     6961 gaacgtcagt tagcagcgaa tgggaggaac tagatgctgc acccggacta cctggacacg
     7021 gacctcggcc ccatcggccg cgtggtaacg agcgaggacc aacccgccac cgcccacgag
     7081 ttctacttct ggaccgccga gaccgaggcc gcccagaacc tggacatcgg tcacatcgtc
     7141 gtcgccgagg ccgaggacgc cacggccatc gcagtcctcg acgacccgcg ccgctactcc
     7201 gacctccagt ccttcctcga cgatttctac gcctacgacg gcgacccatc gctggaagcg
     7261 ctctcggaga gggtagagat cctggttttc agagcccgag tcctcgccac caaacaccgt
     7321 gacgagcgca agaagagcaa gcgcccggtg cgcaccggcc cggtcttctt cgccacgagc
     7381 gccgccatcg agtacgccct cggcaccgac gagttcgccg gacacaagat ccccatgctc
     7441 ctgcacgaga acggcaacga gaaggacggg agcccgcagc gcacgccgct gtacgtggac
     7501 ggcgactacc tgctcggccc cgaggccggc cacctcaaca tcaccggcat gagcggcctc
     7561 tccaccaaga ccagccacgc cctgttcacc atctccagca ccttccagac cgtcaaggac
     7621 aagaaagtcg ccgccctgat gttcaacgtc aagggcgcgg acctcctgtt cctggacaag
     7681 cccgtcgagg tcgacccaca agacgacccg gacctcgccg cccgctacga gaaggccggg
     7741 cagcggggcc tgccgcgcga ggaccgcgag atgtacgcct ccctcggtct cgaaataaag
     7801 ccgttcgaga acctccgcat cttcgcgccg ctagccttcg gcaaggaccg gggcgagcgc
     7861 aaggtccacg ccggggacct gcgcgccaag gacctcaaca ccctgcgccg cgccttcggg
     7921 gaggacagtt gcgtctaccc catcgtgtgg gacctcgaag acgtgctgcc gcacgcgggc
     7981 cgcatcttcg accagaacga tatggatgac aagttcaagg gcttcgtaga gtacctgcgc
     8041 gaccggcacg tccgcaccat gagcgccttc cacgccgagg tggacgaggc cttcgcgtac
     8101 ttcgaggaat ccgagagctc ctactggaac ggccaccatc aggccaccgt cgcgaaggtc
     8161 cgcaaccgtt tccaggtgct gccgagcaag tgcgccgggc tgctggtaaa cggccgggtc
     8221 gagtacggcg acacgccgga ggtggatggg ccgttcgggg atagggagat gcgcgtcgtg
     8281 gacatctcgc acctctcggg cgtgccgcag gacctcgtcg tcaccaaggt cataaacagc
     8341 gtgtgggaga tggccgagca ggggcggctc ggggtggaca agctcgtcat cttcgtggac
     8401 gagctgaaca agtacgctcc atccggctcc cggtccagca gcctcaaaga caccctggta
     8461 gacataagcg cccgcggacg ccacctcaac ctcacgctct tcggggcgca gcagttccgc
     8521 agcaaggttg acgacgaggt cgtcggcaac gccgccacga gcctctacgg gcgcatcggc
     8581 gacgaggagt tgaccaacag cagctaccgt tcgttctcgc agaccacccg cgaggagttg
     8641 ctgcaactgg agaaaggccg cctgctgctg cgccacgctc actacgctgt gccggtcttc
     8701 ggcaccttcc cgcgcccgat ggtgctcatg ggcaagcagg ggac
//
LOCUS       DASSMB010000153        19211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1808787, whole genome shotgun sequence.
ACCESSION   DASSMB010000153 DASSMB010000000
VERSION     DASSMB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 19211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19211
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1808787"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VFJ72_09115"
     CDS             <1..812
                     /locus_tag="VFJ72_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET7479659.1"
                     /translation="AGDLRHEDADGVSIAEAMRANGLDPDLIGEAARSEGSVAAYVEL
                     HIEQGRVLENEGLPVGIVTGIAGPVWLRFTLRGETGHAGSTPMGLRRDALAAAAEIMG
                     VIEEEATRTGTTVGTVGQIEAMPGGINIIPGRVEFSLDLRDIDEGARDGVETRIRERS
                     RKICERRGLELGFEELQRLPPAPCSEEVRRAAREACEEIGTPPFDLPSGAGHDGMQLA
                     GLCPMGMVFVRSRNGISHNPEEWSSREDCAAGARVLYHTVLTLADGELASK"
     gene            977..2122
                     /locus_tag="VFJ72_09120"
     CDS             977..2122
                     /locus_tag="VFJ72_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7479660.1"
                     /translation="MGWRRHLHRNPEVSFHEEKTSQFIYETLESFGGLEISRPTKTSV
                     VARLLGERPGFTLAIRADMDALPIEEENDFEFVSQNPGAMHACGHDGHTAMLLGTAKI
                     LAGMKDRIVGEVRFIFQHAEELLPGGAEEMVEAGVMDGVDAVIGIHLWAPMEIGSIGV
                     TYGPMMAAPDKFQISIKGKGGHAAMPHQAVDSIAIGAQVVTNLQHVVSREIDPLDNVV
                     ISVTKFTGGTTHNVIPGTVEMMGTVRTLDPAVRERVPEVMERVIRGITSAHGAFYEFE
                     YERGYRPVINDDEVTRVIEETVRETFGDETLEIMRPNMGGEDFSAFQQKAPGTFFYIG
                     AGNEEKGIVYPHHHPRFTIDEDALPYGIKMFTNAAFKLLDRNTKEGS"
     gene            2128..2319
                     /locus_tag="VFJ72_09125"
     CDS             2128..2319
                     /locus_tag="VFJ72_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HET7479661.1"
                     /translation="MYLLAALPFIAILGGIVFANRVEPYVLGLPFILFWIVLWVVLTS
                     GIMATIYRIDPANREDDSE"
     gene            2316..3782
                     /locus_tag="VFJ72_09130"
     CDS             2316..3782
                     /locus_tag="VFJ72_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET7479662.1"
                     /translation="MNVALIIIFAFLALAIFLGIRARRGRDMDLEQWSVGGRGFGTIF
                     VFLLLAGEIYTTFTFLGGSGWAYGQGGPAFYILSYGCLAYIMSYWLLPAVWRYANENR
                     LHSQADFFVSKYGSPALGVLVSLVAVVAMVPYLALQIRGLGLIVSAASYGTISATVAS
                     IIGVVAVVVYVIISGIHGSAWTAVLKDIMVLGVVVFLGLYLPFKLYGGIGEMFHQIQA
                     QKPDFLTLPGQGYSPAWFISTVLLTALGFYMWPHTFGSTFSAQNERVFKRNAIFMPLY
                     QLVLLFVFFVGFAAVLKVPGLEGDKVDLSLLMISKDTFSSPVVGLIGATGLLTALVPG
                     SMLLMVSSTLLAKNVYKAFAPSTTEAQVGTLAKILVPFVGLAALLFALSSGQAIVLLL
                     LLGYSLVTQLFPSLLFSLMANNLVTKWGAASGIIVGVATVTYVTVTGTTIGQLLPFLP
                     QAAADTNIGIVALAVNVVVLLVVSFATGGLAVRREQSA"
     gene            3871..4176
                     /locus_tag="VFJ72_09135"
     CDS             3871..4176
                     /locus_tag="VFJ72_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479663.1"
                     /translation="MRKAKRLGTALAASAMLIGATAAPALAGDHLFNGSTSPGADQRG
                     FANPVTANPSGTSGAAAKPATVPGEGNPNAGLDQGTPSVDLSAVSVRSGGNGDPQAQ"
     gene            complement(4220..4891)
                     /locus_tag="VFJ72_09140"
     CDS             complement(4220..4891)
                     /locus_tag="VFJ72_09140"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7479664.1"
                     /translation="MKAVESLRVRALLLAAFLTSIVVAAVLAPAAGAQEGGPETGCTG
                     QIVLDPGHGGTDTGTSNSTYGLLEKDQNLDVALRLEALLEGEGHNVCMTRTGDATLSN
                     NDRYTYANTTGAKLLISIHMNGSTNKSIDYTTTLYGKPRKDQELANVMLDALYPALGI
                     PKRAPSQFASGVLLKSDMPAIIAESVFITNDGEGRLLSDGTGVRQQQIAEALRAGIDN
                     YLATR"
     gene            5088..5912
                     /locus_tag="VFJ72_09145"
     CDS             5088..5912
                     /locus_tag="VFJ72_09145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET7479665.1"
                     /translation="MELRDLESHGEPGALVYRADCVDLMRLMPAGSVDVIFADPPYRL
                     SSGGVTVKSGRISSVDKGAWDRSLGSFEKDHEFNLRWIREARRVLKPDGTIWVTGTHH
                     IIFSIGFALQSLRFKVINDITWQKPDPPPNALRIAFTHAHETLLWASKGRGARHTFNY
                     DLINSPDPASPLSSIWRIPAVPKGEKLCGYHPTQKPLRLVRRALLASTREGDLVFDPF
                     CGSGTTAVAAKELNRFFVGSELEREFAELAARRISAAERGGVLREISGLRLEDDQS"
     gene            5982..7709
                     /gene="cydD"
                     /locus_tag="VFJ72_09150"
     CDS             5982..7709
                     /gene="cydD"
                     /locus_tag="VFJ72_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HET7479666.1"
                     /translation="MNRALFEHVRFARLYVVITALLGFLAAAATVAQMVFLSRIVDRV
                     FLKGAGLASVRDLLAILLAAVIARAALLWVREVAAQRGAVRVKSELRDRLFEHVLRLG
                     PSYTGGERTGELVTTATEGIEKLEPYFARYLPQMVLSASVPLLVVAYVFPRDWSSAAL
                     LLITAPVIPIMMVLVGSYAEEHMQRQWTALSRMGAHFLDVLQGLPTLKTFNRGAAERE
                     RVAAVSEEFRARTMKVLKFAFLSGLVLEFMTAVAIALIAVTLGVRLVSGNMPFEEAFV
                     VLLLAPEFYRPLRELGVHRHAGMEGKAAAERIAEILAIPPPVSGECGPSDHLSGGLTI
                     DLSSVGYTYPGNERPALSDLSLMLPAGTRTAVVGRSGTGKSTLVNLLMRFLDPMEGSI
                     RANGVEVQDLPVDLWRTNVALVPQRPHLFHGSVLENIRIARPEASRAEVERAAELAGA
                     AEFIRRLPKGYDTQVGERGARLSGGEAQRLAIARAFLKDAPVLILDEPTSSLDPESER
                     LVRLAVEKLSEGRTTLIVAHRLNTVYSADKIVVLEAGRLAESGTHAALAGGDGPYARL
                     VGAYGGASA"
     gene            7706..9466
                     /gene="cydC"
                     /locus_tag="VFJ72_09155"
     CDS             7706..9466
                     /gene="cydC"
                     /locus_tag="VFJ72_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HET7479667.1"
                     /translation="MRTFLRLIRFLGPYRWQVALAVFLGVVTVVSNVGLLATAAYVIS
                     AAAIVSYISLLVIQVYLVRIFSVSRSFSRYAERMVSHDVTFRLLGNLRTWFYGRLEPL
                     APARLAGYRSGDLLSRVVRDVEELENVYLRILAPAAIAAVISAFTFALFYIFSPALAF
                     VALGFLTLTGVGVPILVRTLSRGLGRRELELRAELNARIVDGVQGAQDLLAFGAEGAH
                     QMEIAAVGRKLDRVQRKMAFVTGLRDSLNDLLTNLTMIAALVLTIPLVAQGEIKGVYL
                     GFLALVALGAFEAVQPLGTAFQFLGRSLHAGERLFEISDSEPAVRDPEKPYPAPADHT
                     LEFDRVSFQYEQDETAVLEDISFTLRPGSRIAVVGPSGAGKSTLVNLILRFWDPQGGE
                     VRFGGRDIRDYETEDLRSLVGVVAQDTHVFNASLRENLSLARPGADDTVLKEALERAR
                     LSDLVERLPGGLDGYIGEQGARISGGERQRLAVARALLKDAPVLVMDEATANLDPVTE
                     RELLESARSLMLGRSTLVITHRLVDMESMDEILVLDGGRIVERGTHAELRGSGDLYSR
                     MLEVQNGMFAARTSGRIGQN"
     gene            9566..10963
                     /locus_tag="VFJ72_09160"
     CDS             9566..10963
                     /locus_tag="VFJ72_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7479668.1"
                     /translation="MEDPVFISRLQFAFTITYHYLFPQLSMGLALLIFVLKSVYMRGG
                     NEAYNKSARFWGKIFAVTFVMGVVTGIPMEFQFGTNWAAFSQYAGDIIAQTLAMEGAF
                     AFFLESAFVGIFLFGERRFGQRVHWFSSLMVFLGTWASGYFIITTNAWVQHPVGFERV
                     GDGIQLNNYWAVLLNSWMFAQYAHNMGGAVVTGAFVMAGLGAFYLLARQHEEYGRIFL
                     KVGIIAGVVASLWQLFPSGHFSSDQVAKHQPVTLAAMEGQFKSEKPAGLVLVGQPDIK
                     NLRIDNPIVIPRVLSILVYQNWNAEVKGLQAYPKKNWPDNIPLLYYSYHVMVGLGTIF
                     IGIMGLSTFLLWRRRLFESRWMLWVLMLAIPFPFIANTAGWFTAELGRQPWIAYGLLR
                     TADGVSPLVSSGNVLFTLIGFAGMYLIMGLLYIVLMVREVGHGPESEEEMLGLAGDRT
                     DVEPGSEPGTEPRTA"
     gene            10973..12013
                     /gene="cydB"
                     /locus_tag="VFJ72_09165"
     CDS             10973..12013
                     /gene="cydB"
                     /locus_tag="VFJ72_09165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET7479669.1"
                     /translation="METIWFIAVALMVAMYVLLDGFDLGAGTIHLFAARNEAERRTVI
                     RAIGPVWDGNEVWLIAAGGTLFFAFPTLYAAGFSGFYLPLIMVLWLLMIRGLSIELRG
                     HMDNPMWASFWDAAFFVGSALLTFFFGAALGNVVRGVPLNKNGFFFQPLWTNFSPFAK
                     VPGILDWYTILIGLLALTTLIVHGANFIAVKTENDLNARARRISGRFTYATIALTVLA
                     TIATFWVSPRMLEGFNSRPYGYVLPLIAIAGLVGMVLYSGRGRDTAAFLASSAFIIGM
                     LTSTVFGVYPDVLPAVNPENSLTIHNAASSNYSQAVGLVWWTIGIILAAIYFVVIYRL
                     FRGKVRLEDEGY"
     gene            complement(12010..12687)
                     /locus_tag="VFJ72_09170"
     CDS             complement(12010..12687)
                     /locus_tag="VFJ72_09170"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HET7479670.1"
                     /translation="MSELQSRGPSIIFEVLRRPRPALESIGRESRLGSGIAVVAIWAA
                     LGLILTLLSGPGGGLRPEQFPELPPAAFAQLERGLRVFVPLSALLFPFVWWLGVSSLM
                     YLTVRLFGGRASLSSMLAVVGVACAPWVAGYLVQIPLALLQLALEGGGTVPAILGALT
                     FIVSAAALVWHVALVVLGARLVAGTSPGGAAASCALAGLGCVTAAFFLGLTLVTLAVG
                     LSRGFGG"
     gene            12802..13278
                     /locus_tag="VFJ72_09175"
     CDS             12802..13278
                     /locus_tag="VFJ72_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7479671.1"
                     /translation="MLAVVIADTHIPRRAKVLPDALHAHLRRSDLILHAGDLMDPALL
                     EDLGAYAPVRTVRGNLDPADAGLPETLEFEFGSAQVAMIHDSGPKKGRRNRMRRRFPE
                     ARVVIFGHSHIPWLEDADGLLLLNPGSPTDRRRQPEYTFALLRVEGGEVSAEILAL"
     gene            13319..14602
                     /gene="nhaA"
                     /locus_tag="VFJ72_09180"
     CDS             13319..14602
                     /gene="nhaA"
                     /locus_tag="VFJ72_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET7479672.1"
                     /translation="MPGKTIKRLLSPFEQFVHSESAGGISLIFAALVAFVWANSPWAP
                     GYFALREITFGVGFGEWRLEKPLLLWVNDGLMAFFFYLVGLEIKREVLVGELSAPRDA
                     VLAVAAAVGGMILPATIYAAVNLGGEAIGGWGIPMATDIAFALGMMALLGDRVPLSLK
                     VFLTALAIVDDLGAVLVIALFYTDEINTVNLLVTAVALALAFAYGRFGGRNLKVLAFF
                     GVVVWFFVLQSGVHATVAGVLLALATPMKRKILPENLRDELRAGLERGEFEEVEVKVA
                     SLERVLGDAQSPLHRLEHLMHPWVAFLILPVFALFNAGVDLGGGAALTEPVTVGVVLG
                     LIVGKPLGVLGFSWLATRLGIATLPRGVGWSGMAGAGLLAGIGFTMSLFVGGLAFEEN
                     ENLLDGAKLGILSASVVAAILGLLVLGWRKSTAGG"
     gene            14606..16630
                     /locus_tag="VFJ72_09185"
     CDS             14606..16630
                     /locus_tag="VFJ72_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7479673.1"
                     /translation="MGIAGDIALILIAAFVGGVIARWLGLPLILGYIVAGVFVGPNTG
                     GPTVGEIHDIELLAEIGVALLLFTIGLHFPLGELVPVKRVALIGTAIQMALTIAFGYG
                     LGQLLGLGWAEAVWFGALLSVSSTAVVLKTLDEQGFLNTLASRVIIGMLIVQDIAVVP
                     LLILLPELGNVGQGLSRLGIAALEAAGFVAVMAVLGVRVFPWLMAKVVGWGSRELFLI
                     SVVAVGLGVGYGTYLFGLSFAFGAFIAGMVLSQSDYSHQALADIEPLRDVFAMLFFVS
                     VGMLIDPAFLWREAGTILLVVLLVFLFKGALFAGITRSFGYGNIAPFAVGLGLFQVGE
                     FSFVLARVGVSDGAISGRLYSIALTTAVVTMALTPFATRLAPVLYRRWRERFPKELLT
                     TYNLPEMGLKDHVVVAGHGRVGRFVARMLHGLGQPLVVVDLNPDRVEEARNSGVPVVY
                     GDASAEPVLEAAGIKRARVVICTVPDPLGARLVIERIRRMSPEIHIVARSTTPEQLED
                     LGALGVYEAVQPEFEAGLEIGRQALVHLGFGAGEVHRFSDRVRGELYAPISDPARGGH
                     LDGLRRATVMIETDWVDLPEESPLAGRTIGDLAVRTRTGASIVAVVRGREVFTNPGPE
                     FQLAAGDTLGALGTSGQRSAFLEMIEAKPDTEPDEDQERASVPDKDRPPV"
     gene            16638..17717
                     /locus_tag="VFJ72_09190"
     CDS             16638..17717
                     /locus_tag="VFJ72_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HET7479674.1"
                     /translation="MAEGRRERDWSGFEVVFGPEGEAVRRIRLAILMLLGIIVVGTAG
                     YITLGWSFLDSLYMTVITVGTVGFEEVNDLDNSVAGRLWTIILIISGVAVLGYATTSL
                     VALAVEGTVRNYFRSRRMRAEISKLSGHYILCGYGRVGRQVAAEFALDGVPFVVIDQD
                     PRIVEECLERGYLALPGEASDDGTLEEAGVMRARGLVAAVDSDADNVFVVLSARKLNP
                     ALHIVARASSDGSAAKLEIAGADRTLSPYAVGGRRLASLATQPMVVDFLDVVTRGEEG
                     IEFRLEEFMVPKEPAIADHTIGELKIGERTGAIVLAFRTADGKFDTTPSAGDRLHAGD
                     TLIVLGSREQITRLEQLMRGEKITE"
     gene            complement(17745..17996)
                     /locus_tag="VFJ72_09195"
     CDS             complement(17745..17996)
                     /locus_tag="VFJ72_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479675.1"
                     /translation="MSCAPERSSPASPDCLERYASLLLLAVQVAFMGPVSRPAYMGFR
                     DIALARREDISRLQEFVRDSDGPVLADETLDRGDAACDP"
     gene            complement(18027..18170)
                     /locus_tag="VFJ72_09200"
     CDS             complement(18027..18170)
                     /locus_tag="VFJ72_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479676.1"
                     /translation="MPVMVASGVAFLVLAVRSRMESRWLVVPYLLGALVTALLVGKVE
                     LVR"
     gene            complement(18455..>19211)
                     /locus_tag="VFJ72_09205"
     CDS             complement(18455..>19211)
                     /locus_tag="VFJ72_09205"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HET7479677.1"
                     /translation="GRRSGGGGIENLGTVVIRNSIVSGNSAGFGGGISNDRGQVLVES
                     STFSGNKADSGAGGIGNNQGNLTVRNSTFSANSDLGTADSFIGGGGGIQNFLGRVSIV
                     DSTFSGNVSSGSSSDGGGINNVINPVSGDSGKIFLRDTIVAKNQAPTGPDASGPFASG
                     GYNLIGDTSGATGFGATDLQNTPSGLDPRGLRDNGGPTMTIALVGGTPPVDAVRGDCT
                     PPTTDQRGIRRPQDGDSDGISRCDIGAYERRAQ"
BASE COUNT         3208 a         6034 c         6397 g         3572 t
ORIGIN      
        1 acgctggcga tttgcgacac gaagacgcgg acggcgtatc catcgccgag gcgatgcgcg
       61 cgaacggtct ggacccggat cttatcgggg aggccgctcg ttcggaggga tcggtcgcgg
      121 cttacgtcga gcttcatatc gagcagggcc gggtgctgga gaacgaggga ctgccggtcg
      181 ggatcgtgac cgggatagcc ggtccggtgt ggctccggtt cacgctccgg ggcgagaccg
      241 ggcacgctgg ttcgacgccg atggggctca ggcgcgacgc tctggcagcg gcggcggaga
      301 tcatgggcgt gatcgaggag gaagccactc gaaccgggac gaccgtggga accgttggtc
      361 agatagaggc gatgcccggc ggcatcaaca tcatccccgg acgggtcgag ttctcgctgg
      421 acctgcgcga tatagacgag ggcgcgcggg acggtgtgga gacgcgcatc agggagcggt
      481 cgcgcaagat ctgcgagcga cgcggcctgg agctagggtt cgaggagttg cagcggcttc
      541 cgccggcgcc atgctcggag gaggtgcgcc gggccgccag agaggcctgc gaagagatcg
      601 gaacacctcc cttcgacctt ccgagcggcg ccgggcacga tgggatgcaa ctcgcgggat
      661 tatgtccgat gggtatggtc ttcgtccgct ccaggaacgg aataagccac aaccccgagg
      721 agtggagttc cagggaagat tgcgcagccg gcgcgagagt cctctaccac accgtgctga
      781 ccctcgcgga tggagaactg gcgtcgaagt agacgtggga tccagggtgc cggatctcta
      841 tacacgagac caaagacagg taaggcagga taaattatgg tagtttcgtc cttgcatagg
      901 tacggcgaac aagggggaat cagccattct gaagtcatcg ctcgaagatc agatagcaga
      961 cgtcaaagac gaggtcgtgg ggtggaggcg tcatctacac cgcaaccccg aggtctcctt
     1021 tcacgaggag aagacttccc aattcatcta cgaaacgctt gaatccttcg gtggcctgga
     1081 gatctcccgg cccacgaaga ccagcgtggt ggcccggctt ttgggggagc gtccgggatt
     1141 cacgctcgct atccgggcgg acatggatgc cctgccgata gaagaggaga acgacttcga
     1201 gttcgtctcc cagaaccccg gcgcgatgca tgcctgcggc cacgacgggc atacggcgat
     1261 gcttctcggg acggcgaaga tactcgccgg gatgaaggac cggatagtcg gcgaggtccg
     1321 cttcatcttt cagcacgccg aggagttgct ccccggcggt gccgaggaga tggtcgaggc
     1381 cggcgtcatg gacggcgtcg acgctgtaat agggatacac ctgtgggcgc cgatggagat
     1441 cggcagtatc ggggtcacct acggcccgat gatggccgcg ccggacaagt tccagatctc
     1501 gatcaagggc aagggcgggc acgcggcgat gccgcaccaa gcagtggact ccatcgccat
     1561 aggggcgcag gtggtgacga acctccagca cgtcgtctcg cgagagatag atccactgga
     1621 caacgtggtc atctcggtca cgaagtttac cggcggtacg acgcacaatg tcatcccagg
     1681 tacggtggag atgatgggca ccgtgcgcac cctcgacccg gcggtccgcg agcgcgttcc
     1741 cgaggtcatg gagcgggtca tcaggggaat cacgtccgcc cacggcgcct tctacgagtt
     1801 cgagtacgag cgcggctacc ggccggtcat caatgatgac gaagtgaccc gcgtaataga
     1861 ggagacggtt cgggagactt tcggagacga gacgctcgaa ataatgcgtc ccaacatggg
     1921 cggcgaggac ttctcggcgt tccagcagaa ggctccggga acgttcttct acatcggggc
     1981 cgggaatgag gagaagggga tcgtctatcc gcaccatcac ccgcgcttca cgatagacga
     2041 ggacgccctg ccctacggga tcaagatgtt caccaacgcg gcgttcaagc tgctcgatag
     2101 gaataccaag gaggggtctt gaagttaatg tatctgctgg cggcgctccc gtttatcgcg
     2161 atactcggcg gcatagtctt cgccaaccgg gtcgagccat acgtgttggg cctgccgttc
     2221 atactgttct ggatcgtgct gtgggtcgtg cttacctcgg ggatcatggc gaccatctac
     2281 cgtatcgacc ccgccaacag ggaggatgat tccgaatgaa cgtcgcgctg ataataattt
     2341 tcgccttcct cgccctcgcc attttcctcg gcatccgggc gcggcgcggc agggacatgg
     2401 acctcgaaca gtggagtgtc gggggacggg gcttcgggac aatcttcgtc ttcctgctcc
     2461 tggccggtga gatctacacg accttcactt tcctcggcgg cagcggctgg gcctacgggc
     2521 agggcggtcc tgccttctac atcctctcct acggatgcct ggcgtacatc atgtcctact
     2581 ggctgctccc ggcggtctgg cgctacgcca acgagaaccg tctccactcg caggcggact
     2641 tcttcgtgag caagtacggc agccccgctc tcggtgtcct ggtctcgctc gtcgcggtgg
     2701 tcgcgatggt tccttatctg gcgttgcaga tccggggcct cgggctcata gtctctgcgg
     2761 cctcctacgg aaccatatcc gcgaccgtgg cctcgatcat cggggtggtc gccgtggtcg
     2821 tctacgtgat catcagcggc attcacggct cggcctggac cgccgtcctg aaggacatca
     2881 tggtgctcgg ggtcgtggtg ttcctcggcc tctacctgcc cttcaagctc tacggcggca
     2941 tcggagagat gttccaccag atccaggccc agaaaccgga cttcctgacg cttccgggcc
     3001 agggatacag cccggcgtgg ttcatctcta cggtcctcct gacagcgctc ggtttctaca
     3061 tgtggccgca caccttcggc tcgacgttct cggcgcagaa cgagcgggtc ttcaagcgca
     3121 acgctatctt catgccgctc tatcagctcg tgctgctctt cgtcttcttc gtcggcttcg
     3181 cggcggtgct gaaggttccc ggcctcgaag gggacaaggt ggatctctcc ctgctgatga
     3241 tctccaagga cactttcagc tctccggtcg tgggtctcat cggggcgacg ggtctcctta
     3301 cagccctggt ccccggctcg atgctcctta tggtgtcatc caccctgctg gccaagaacg
     3361 tatacaaggc gttcgctcca tcgactaccg aggcgcaggt gggtacgctc gccaagatac
     3421 tggtcccctt cgtagggctt gcggcccttc tcttcgcgct cagtagcggg caggccatcg
     3481 tgcttctgct gctgctcggt tacagcctcg tgacgcagct cttcccgtcc ctgctcttca
     3541 gcctgatggc taacaacctc gtcaccaagt ggggagccgc ttccgggatt atcgtcggcg
     3601 tcgcgaccgt cacctacgtg accgtaaccg ggaccaccat cggccagctc ttgccgttcc
     3661 tgccgcaggc tgcggcggac accaacatcg gcatagtagc attggctgtt aacgtggtag
     3721 tgctcctcgt ggtgagcttc gccacggggg gactagccgt gcgccgtgag cagagcgcct
     3781 gagaatatcc cggcgatccg gaccccgtaa actgctttgt cgagcggcgc gtataaagtg
     3841 ccgtagcgca aaccgaaaga aggagaaacc ttgcgtaagg caaagaggct cgggaccgcg
     3901 ctggcggcct cggcgatgtt gattggcgcc accgcggcgc cggctctggc gggcgaccat
     3961 ctcttcaacg gttctacctc tccgggggcg gaccagcgag gcttcgcaaa ccccgtgacc
     4021 gcgaatccgt ccggcacctc cggagccgcc gcgaagcccg ccaccgtacc cggcgagggc
     4081 aaccccaacg cgggcctcga ccagggcacg ccctccgtgg acctgagcgc ggtcagcgtc
     4141 cgcagcggcg ggaacggtga cccccaggcg cagtagataa tctgcggccc ggagaggccg
     4201 ggccgcaggt ttggcctctc tagcgggtag ccaggtagtt gtcgatcccc gctcgcaggg
     4261 cctcggctat ctgctgctga cgtactccgg tgccgtcgga caacaggcgg ccttctccgt
     4321 cgttcgtaat gaagacgctc tcggcgatga tcgcgggcat gtcgctcttg agcagcacgc
     4381 cggaggcgaa ctgggagggg gctctcttgg ggatgcccag ggccgggtag agggcgtcca
     4441 gcatcacgtt cgccagctcc tggtccttgc ggggcttgcc gtaaagggtg gtggtgtagt
     4501 ctatggattt gttggtggag ccgttcatgt ggatggagat gaggagcttc gccccggtgg
     4561 tattcgcgta ggtgtagcgg tcgttgttcg agagggtcgc gtctcccgtg cgcgtcatgc
     4621 agacattgtg accctctcct tcgaggagag cctccagcct cagggccacg tccaggttct
     4681 ggtctttctc cagcaggccg tatgtgctgt tcgacgtccc ggtatccgtc ccgccgtggc
     4741 cgggatccag gacgatctgc cccgtacagc ctgtttcggg acctccttcc tgcgcccctg
     4801 ccgcgggagc aaggaccgcc gcgacgacaa tgcttgtaag aaacgccgcc agcagcaatg
     4861 cgcgaaccct gagcgactcg acagctttca tcttgttcgc cctttcctcc cctgacagcg
     4921 aacgggaagc aaggcccgcc gcacacacag cattacccgg tagattaccc gaacggcaga
     4981 gccgtgagaa tagctcaaac gggctattcc ccggtggacg taagaattgt tgccacgggg
     5041 caaccgagtg tgtctcgctg gaactggtct tctcctaaaa tagccgtgtg gaactcagag
     5101 acctcgaatc tcacggcgag ccgggcgcgc tggtctaccg ggcggactgc gtggacctca
     5161 tgcgcctgat gcccgccggt agcgtggatg tcatcttcgc cgacccgccc taccggctat
     5221 caagcggggg agtaacggta aagagcggca ggatctcctc cgtggacaag ggggcctggg
     5281 accgctcgct cggcagcttc gagaaggacc acgagttcaa cttgcgctgg atccgggagg
     5341 cccgccgcgt gctcaagccg gacggtacga tctgggtgac cgggacgcat cacataattt
     5401 tcagcatcgg cttcgcgctc cagtcactca ggttcaaggt gataaacgac ataacctggc
     5461 agaaaccaga cccgcccccg aacgccctgc gaatagcctt cacccacgct cacgagacgc
     5521 tcctgtgggc cagcaagggt agaggcgccc gccacacctt caattacgac ctcataaaca
     5581 gcccggaccc cgcctctccg ttgtcctcta tctggcgcat accggccgtg ccgaaggggg
     5641 agaaactttg cggctaccat cccacgcaga agcctctgcg tctagtgcgg cgggcgctgc
     5701 tcgcctcgac gcgggagggc gacctagtct tcgacccgtt ttgcggatcg gggacgacgg
     5761 cggtcgcggc gaaggagttg aaccgtttct tcgtcgggtc ggagctggag agggagttcg
     5821 cggagcttgc ggcgcgccgg atctccgccg ccgagcgtgg cggcgtgctg cgggagatct
     5881 ccggcctccg tttggaggat gatcaatctt gatcaatccg cgtgccggat catgctctac
     5941 catgacggca tggcgagcgg attcgactta tctgacggcg tatgaatagg gcgctcttcg
     6001 agcacgtccg cttcgcgcgg ctctatgttg ttatcacggc cctgttgggt tttctggcgg
     6061 ccgccgctac cgtggcgcag atggtcttcc tgagcaggat cgttgaccgg gtcttcctga
     6121 aaggcgcggg cctggcgtcg gtgcgggatc tgctggccat cctgctcgca gccgtgatcg
     6181 cgcgggcagc gttgctgtgg gtgcgggagg tggccgccca gcgaggcgcg gtgcgggtga
     6241 aatccgagct gcgcgacagg ttgttcgagc acgtcctgcg gctcggaccc tcctacacgg
     6301 gcggcgagag gaccggggag ctggtgacga cggcgacgga agggatcgag aagctggaac
     6361 cttacttcgc ccgctacctg ccgcagatgg tcctgagcgc gtcggtgccg ctattggtgg
     6421 tggcgtacgt gttcccgagg gactggtcga gcgcggcgct gctcctcata acggcgccgg
     6481 tcatcccgat catgatggtc ctcgtaggtt cctacgccga ggagcacatg cagcgccagt
     6541 ggacggccct ctcgcgcatg ggcgcgcact tcctcgacgt cttgcagggg ctcccaacgc
     6601 tcaagacgtt caaccggggg gccgcggagc gggagcgggt cgcggcggtg agtgaggagt
     6661 tccgggcgcg cacgatgaag gtgttgaagt tcgccttcct ctcggggctg gtgctggagt
     6721 tcatgacggc cgtcgccatt gcgctcatcg ccgtcacgct cggtgtgcgg ctggtctccg
     6781 ggaacatgcc gttcgaggag gcgttcgtgg tcctgctcct cgcgccggag ttctaccggc
     6841 cgctccgcga gctgggcgtc caccggcacg ccgggatgga gggcaaggcc gccgccgagc
     6901 gtatagccga gatcctggct atccccccgc cggtgagcgg ggagtgtggc ccctcggacc
     6961 atctctcagg cgggttgact atagatctct ccagtgtcgg ctacacgtat cccgggaacg
     7021 agcgtccggc gctctcggac ctctcactca tgctgcccgc cggtacgcgg accgccgtgg
     7081 tcggacgtag cgggacgggg aagagcacgc tcgtgaacct cctgatgcgc tttctggacc
     7141 cgatggaggg aagtattcgg gcgaacggtg tggaggtaca ggatctgccg gttgacctgt
     7201 ggcggacgaa cgtcgcgctg gtgccgcaga ggccgcacct gttccacggg agcgtgctgg
     7261 agaacatccg gattgcgcgg cccgaggcga gccgggcgga ggtcgagcgg gcggcggagc
     7321 ttgcgggagc ggcggagttc atccggcggc ttccgaaagg atacgacact caggtcgggg
     7381 agcgtggggc gcgcctgagc gggggagagg cgcagaggct cgcgatagcc cgcgccttct
     7441 tgaaggacgc gccggtcttg atcctggacg agccgacatc gagcctcgac ccggagagcg
     7501 agcgtctggt ccggctagcc gtggagaaac tgtccgaggg acggacgacc ctgatagtcg
     7561 cccaccgcct caacaccgtc tacagcgcgg acaagatagt cgtcctcgaa gccgggcggc
     7621 tggcggagag cggcacccac gccgcgctcg ccggtggcga cggcccctac gcccggctcg
     7681 tgggcgccta tgggggcgca tcggcgtgag gactttcctg cgcctgatcc gcttcctggg
     7741 tccctaccgc tggcaggtcg cgctggcggt cttcctgggc gttgtgacgg tggtgagcaa
     7801 cgtggggctg ctggcgacgg cggcgtatgt catctcggcg gctgcgatag tctcttacat
     7861 ctcgctgctc gtcatccagg tctacctggt ccgaatcttc agcgtctccc gctctttctc
     7921 gcgctacgcc gaacgcatgg tctcccacga cgtaaccttc cggctgctcg gcaacctgcg
     7981 gacctggttc tacggacgcc tggaacctct ggctccggcc cggctcgcgg gctaccgcag
     8041 cggtgacctg ctctcccgcg tcgtgcgcga cgtcgaggag ttggagaatg tctacctccg
     8101 aatcctggct ccggccgcca tcgccgcggt catctccgcc ttcaccttcg cgctcttcta
     8161 tatcttcagc ccggcgctgg cgttcgtggc tctggggttc ctgaccctga ccggcgtcgg
     8221 ggtcccgatc ctggttcgga cgctctccag agggctcgga cggcgggagt tggagcttcg
     8281 cgcggagctg aacgccagga tcgtggatgg tgtccaggga gctcaggatc tgctagcttt
     8341 cggcgcagaa ggcgcgcacc agatggagat agccgccgtg ggccgcaagc tcgaccgggt
     8401 gcagaggaag atggccttcg tgacgggcct gcgggactcg ctgaacgacc tgctgacgaa
     8461 cctcaccatg atagccgcgc tagtcctgac gataccgctc gtcgctcagg gagagataaa
     8521 aggcgtctac ctgggattcc tggcgctcgt ggctctaggg gctttcgagg ccgttcagcc
     8581 gctcgggacg gccttccagt ttctcgggcg ctcgctccac gccggggagc ggctattcga
     8641 gatctccgac tccgagcccg ccgtgagaga cccggagaag ccgtatcctg ctcccgcgga
     8701 ccacacgctg gagttcgacc gggtgagctt ccagtacgag caggacgaga ccgccgtcct
     8761 cgaagacatc tctttcacgc tgaggccggg gagcaggatc gcggtcgtcg ggccgagcgg
     8821 ggcggggaag agcacgctgg tcaacctgat cctgcgcttc tgggacccgc agggaggcga
     8881 ggtccgcttc ggcggacgtg acatccgcga ctacgagacc gaagacctgc gctcgctcgt
     8941 gggcgtagtc gcccaggaca cgcacgtctt caacgcctcc ctgcgcgaaa acctttcgct
     9001 cgcgcgtccc ggagccgacg acacggtgct gaaggaggct ctggagaggg cgcggctctc
     9061 cgatctggtg gagcgtctgc cgggtgggct ggatggatac attggggagc agggagcgcg
     9121 gatctccggc ggcgagcgac agcggctcgc ggtggcgcgg gcgctcctaa aggatgcgcc
     9181 ggtcctcgtg atggacgagg ccacggcgaa cctggacccc gtaaccgagc gggagctcct
     9241 ggagagcgcg cgaagcctga tgctgggtcg ttcgacgctc gtgattacgc accggctagt
     9301 ggatatggag agcatggacg agatcctggt cctcgacggt ggacgcatcg tcgagcgcgg
     9361 tactcacgcg gaactgcgcg gctccggcga tctctactcc cggatgctcg aagtgcagaa
     9421 cgggatgttc gcggcccgga cttccggccg aatcggtcaa aactgatcaa ttttgatcaa
     9481 gaacccgact aaagatctgg ttacggtgga acgcaggatt ttatgcgagc ataatgcgta
     9541 gcggtaggcg agcgtaggag gcgaagtgga agacccggta ttcatctcac ggctgcagtt
     9601 cgctttcacc atcacctacc attacctctt cccgcagcta agcatggggc tggcgctcct
     9661 catcttcgtc ctcaagtccg tgtacatgcg gggcggcaac gaggcgtaca acaagtcggc
     9721 gaggttctgg ggcaagatct tcgcggtcac cttcgtcatg ggcgtcgtca ccggcatccc
     9781 gatggagttc cagttcggga ccaactgggc cgcgttctct cagtacgctg gggacatcat
     9841 cgcccagacc ctcgcgatgg agggagcatt cgccttcttc ctggagtcgg cgttcgtcgg
     9901 gatcttcctg ttcggggagc ggcgcttcgg gcagcgggtc cactggttct cctcgctcat
     9961 ggtcttcctc gggacgtggg cgtccggcta cttcatcatc acgacgaacg cctgggtcca
    10021 gcacccggtc ggcttcgaga gggtcgggga cgggatacag ttgaacaact actgggccgt
    10081 ccttttgaac tcgtggatgt tcgcccagta cgcgcacaac atgggcgggg cggtggtcac
    10141 gggtgcgttc gtgatggctg gtctcggggc gttctacctg ctcgcccgcc agcacgagga
    10201 gtacggcagg atcttcctga aggtcgggat aatagcgggc gtcgtggcca gcctgtggca
    10261 gctcttcccg agcgggcact tctcaagcga tcaggtcgcc aaacaccagc cggtaacgct
    10321 cgccgcgatg gaggggcagt tcaagtccga gaaacccgcc ggcctcgtcc tcgtcggcca
    10381 gccggacata aagaacctgc ggatagacaa ccccatcgtc atccccaggg tccttagcat
    10441 cctggtctac cagaactgga acgccgaggt gaaggggctc caggcctacc ccaagaagaa
    10501 ctggccggac aacatcccgc tcctctacta ctcctaccac gtcatggtgg gactcgggac
    10561 gatcttcatc gggatcatgg gcctctcgac cttcctgctc tggcggcgca ggctcttcga
    10621 gtcgcgctgg atgctctggg tcctcatgct cgccatcccg ttcccgttta tcgccaacac
    10681 cgccggatgg ttcacggcgg agctagggag gcagccctgg attgcctacg gtctcctgcg
    10741 gaccgccgac ggcgtctcgc cgctcgtctc ctccgggaac gtgctcttca cgctcatagg
    10801 cttcgctggg atgtacctga tcatgggcct cctctacatc gtgctgatgg tccgcgaggt
    10861 cggccacggc cccgagtccg aggaggagat gctgggcctc gcgggagata ggacggatgt
    10921 ggagcctggt tcggaacccg gaacggaacc ccggacggcg tagggaggaa cgatggagac
    10981 tatctggttt atagcggtgg cgttgatggt ggcgatgtat gtactcctgg acggcttcga
    11041 cctcggcgcc gggacgatac acctcttcgc cgcccgtaac gaggcggagc ggcgcacggt
    11101 catccgcgcc atcgggccgg tgtgggacgg aaacgaggtc tggctcatcg ccgccggcgg
    11161 cacgctgttc ttcgccttcc caaccctgta tgccgccggg ttctccgggt tctacctgcc
    11221 gctcatcatg gtgctgtggc tcctcatgat ccggggcctc tccatagagc ttcgggggca
    11281 catggacaac ccgatgtggg cctcgttctg ggacgcggcg ttcttcgtcg ggagcgcgct
    11341 actcacgttc ttcttcggcg ccgccctggg caacgtggtg cgcggcgtcc cgctcaataa
    11401 gaacggcttc ttcttccagc cgctgtggac gaacttcagc cccttcgcca aggttcccgg
    11461 catcctggac tggtacacga tcctgatcgg tctgctcgcc ctcacgacgc tcatcgtcca
    11521 cggcgcgaac ttcatcgccg tcaagaccga gaacgacctg aacgcccgag cgcgcaggat
    11581 ctcaggccgt ttcacctacg cgaccatcgc tctaaccgtc ctcgccacca tcgctacctt
    11641 ctgggtcagc ccccgcatgc tcgaaggctt caacagccgc ccctacgggt acgtgctgcc
    11701 cctaatcgcc atagccggtc tcgtcgggat ggtcctctac agtggcaggg gacgcgacac
    11761 cgccgcgttc ctagcatcca gcgccttcat aatcgggatg ctcaccagca ccgtcttcgg
    11821 cgtctacccg gacgtgctgc ccgccgtcaa ccccgagaac agcctcacca tccacaacgc
    11881 cgcgtcttcc aactacagcc aggcggtcgg tcttgtctgg tggaccatcg gcataatcct
    11941 cgccgccatc tacttcgtcg tcatctaccg cctcttccgc ggcaaggtgc gcctggagga
    12001 cgagggctac tagcctccga accccctgga gagcccaacg gccagcgtga cgagcgtgag
    12061 ccccaggaag aacgccgctg ttacgcatcc cagtcccgcc agcgcgcagg aagccgccgc
    12121 gccgccgggg ctcgtcccgg cgacgagccg ggcgcccagg accaccagcg ccacgtgcca
    12181 cactagcgcc gcggcggaca cgatgaaggt gagagcgccc agtatcgccg gaaccgtacc
    12241 gcctccttcc agcgcgagct gcaacaaggc cagcggtatc tggaccagat acccggctac
    12301 ccacggagcg cacgccaccc cgacgaccgc cagcatggaa gagagcgagg cccgtcctcc
    12361 gaacaggcgc accgtcaggt acatcaggga cgagacgccc agccaccaca cgaacgggaa
    12421 caggagggcc gacagcggga cgaagacccg cagcccgcgc tctagctgcg cgaaggcggc
    12481 cggcggcaac tccgggaact gctccggccg caatcctccg ccgggtcccg agagcagcgt
    12541 caggatgagg cctagagccg cccatatcgc cacgaccgca atcccggagc cgagtctgct
    12601 ctctcttcca atagactcca gggccgggcg gggtctccgc aagacctcga agattatgct
    12661 gggaccgcga gactgcaact cggacaaccc ctctccgttc acgggcgaga cgtgcgacgt
    12721 ggcgattcta gcagaggcgc gtacgcgctc atggcaactc agctaccatg accccgctca
    12781 ccgaacgcgg aggaaagagg cttgctcgcc gttgtcatcg ccgacacgca cataccccgc
    12841 cgggcgaaag tgttgccgga tgctctgcac gcgcatctcc ggcgctccga cctgatcctg
    12901 cacgccggcg acctgatgga cccggcgctc ctcgaagatc tcggcgctta cgctcccgtc
    12961 aggacagtgc gagggaacct ggacccggcg gatgccggat tgccggagac gctggagttc
    13021 gagttcggca gcgcgcaggt ggccatgatc cacgactccg gccctaagaa ggggcggcgc
    13081 aaccgcatga ggcgccgctt cccggaggcg cgtgtggtca tcttcggaca ctcgcacata
    13141 ccctggctgg aggatgcgga cgggctcttg ctgctcaacc ccggcagccc gacggacagg
    13201 cggcgccagc ccgaatacac gttcgcgctg ctgcgcgtcg agggtggaga ggtctccgcg
    13261 gagattttag ctctgtagca ttgtctttga ctcatttcgc caatggtaag atcgaagcat
    13321 gccgggaaag accatcaagc gcctgctgag tcccttcgag cagttcgtac acagcgagag
    13381 cgccggcggt atctccctga tcttcgccgc cctcgtcgcc ttcgtctggg ccaactctcc
    13441 ctgggccccc ggctacttcg cactccggga gatcaccttc ggcgtggggt tcggggagtg
    13501 gcgtctggag aagccgctcc tgctctgggt gaacgacggg ctgatggcgt tcttcttcta
    13561 cctggtcggg ctggagataa agcgcgaggt cctcgtcggc gagctgtcgg cgcctcggga
    13621 cgcggtcctg gcggttgcgg cggccgtggg cggcatgatc ctgccggcta ctatctacgc
    13681 ggccgtaaac ttgggcggcg aggccatcgg cggatggggc atcccgatgg ctacggatat
    13741 cgcctttgcg ctggggatga tggccctgct cggcgaccgg gtgccgcttt ctctaaaggt
    13801 tttcctgacg gctctggcga tcgtggacga cctgggcgcg gtgctcgtga tcgcgctgtt
    13861 ctacaccgat gagatcaaca ccgtgaacct cctcgtgacg gcggtcgcac tggcgctggc
    13921 ttttgcctac gggcggttcg gcggacgcaa cctgaaggtc ctggcgttct tcggggtggt
    13981 ggtgtggttc ttcgtgctcc agtctggcgt ccacgcgacg gtcgccggcg tgctcctggc
    14041 cctggcgacg ccgatgaagc gcaagatcct gccggagaac ctccgggacg agctgcgggc
    14101 cggtctggag cgcggggagt tcgaggaggt agaggtgaag gtggcgagcc tcgaacgggt
    14161 gctcggtgat gctcaaagtc cgctgcaccg cctggagcat ttgatgcatc cgtgggtggc
    14221 gttcctgata ctgccggtct tcgccctgtt caacgcgggc gtagacctcg ggggtggagc
    14281 ggccctcacc gagccggtca cggttggcgt agtgctgggc ctgatcgtcg gcaagccgct
    14341 cggcgtgctg ggcttctcct ggctcgccac gaggcttggg atcgccacgc tccccagggg
    14401 cgtgggctgg agcggaatgg cgggggccgg actgctcgcg ggcatcggat tcacgatgtc
    14461 gctcttcgtc ggtggcctgg ccttcgagga gaacgaaaat ctactggacg gggccaagct
    14521 cgggatactc tccgcctcgg tcgtggcggc cattctgggg ctgctggtgc tagggtggag
    14581 aaagtccacg gccggcggct agcgcgtggg catcgcgggc gacatagcgc tcatcctgat
    14641 tgcggccttc gtgggaggcg tgatcgcgcg ctggctcggg ctgccgctca tcctgggcta
    14701 catagtggcg ggggttttcg ttggaccgaa cactggcggg ccgacggtcg gagagatcca
    14761 cgacatcgag cttctcgcgg agatcggggt ggccctgctc ctctttacca tcgggctgca
    14821 cttcccgctc ggggaactgg tgccggtgaa gcgtgtcgct ctcattggga ccgctataca
    14881 gatggcgctc accatcgctt tcggctacgg gctaggccaa ttgctcggcc tcggctgggc
    14941 ggaggccgtg tggttcgggg ctcttctctc ggtgtcgagc acggcggtgg ttctgaagac
    15001 actagacgag caggggtttc tgaacacgct cgccagccgg gtcatcatcg ggatgctcat
    15061 agtgcaggac atcgccgtgg tgccgctcct gatcctgctg cccgagctgg gcaacgtcgg
    15121 ccagggactc tcccgactcg gtatagcggc gctcgaagcc gccggtttcg tcgccgtgat
    15181 ggcggtgctc ggcgtgcggg tcttcccgtg gctgatggcg aaggtcgtgg gatggggctc
    15241 gcgggagctt ttcctcatat ccgtcgtcgc cgtcgggctt ggggttggct acggaacgta
    15301 tctcttcggt ctctcgttcg ctttcggggc cttcatcgcc gggatggtcc tctcccagtc
    15361 ggactacagc caccaggccc tcgccgacat agagccgctg cgcgacgtgt tcgccatgct
    15421 gttcttcgtc tcggtcggga tgctaataga cccggcattc ctgtggcggg aggcgggtac
    15481 gatccttctg gtcgtgctgc tcgtgttcct gttcaagggc gcgctgttcg ccgggatcac
    15541 acgctccttc gggtacggga acatcgcccc gttcgcggtg gggctcggtc tcttccaggt
    15601 cggggagttc tcgttcgtgc tcgcgcgcgt cggcgtgagc gacggcgcca tctccggcag
    15661 gctctactcc atagccctga ccaccgccgt cgtcacgatg gccctgacgc ccttcgccac
    15721 gcgcctcgcg ccggtcctgt accgccggtg gcgcgagcgt ttcccgaagg agcttctcac
    15781 gacctacaac ctgccggaga tgggtctgaa agaccacgtc gtggtggccg gacacgggag
    15841 ggtcgggcgg ttcgtcgcgc ggatgctgca cgggctcggg cagccgctcg ttgtcgtgga
    15901 cctcaacccg gaccgggtgg aggaagccag gaactctggg gtgccggtcg tctacgggga
    15961 tgcttctgcg gagccggtgc tggaggcggc cgggataaag cgggcgcggg tcgtgatctg
    16021 cacggtcccc gatccgttgg gcgcgcgcct cgtcatagag cgcattcggc ggatgagccc
    16081 ggagatccac atcgtcgccc gctccacgac gccggagcag cttgaggacc tgggggctct
    16141 cggtgtctac gaggcggtac agccggagtt cgaggcgggg ctggagatcg gacgccaggc
    16201 tctcgttcac ctgggcttcg gtgccggaga ggtgcatcgc ttctccgacc gcgtgcgcgg
    16261 cgagctgtac gctcccatct ccgaccccgc gcgcggcgga cacctcgacg gtctgcgccg
    16321 ggccacggtc atgatcgaga ccgactgggt ggacctgccg gaggagagcc cgctcgccgg
    16381 acgcaccatc ggcgacctcg cggtgcgtac caggaccggg gcatctatag ttgccgtcgt
    16441 gcgcggcagg gaggtcttca ccaatccagg acctgagttc caactcgccg ctggcgacac
    16501 actcggggcg ctaggcacct ccggacagcg gagcgccttc ctggagatga tagaggcgaa
    16561 gcctgataca gagcctgatg aggaccagga gagggcttcc gttcccgata aggaccggcc
    16621 gccggtataa tccgcggatg gccgagggga gaagagagag ggactggagc gggttcgagg
    16681 ttgtcttcgg cccggagggt gaggctgtaa ggcgcatccg gctggcgatc ctgatgctcc
    16741 tgggcatcat cgtcgtgggg acggcgggat acatcaccct cggctggagc ttcctcgact
    16801 cgctgtacat gaccgtcatc accgtaggga ccgtgggttt cgaggaagtg aatgacctcg
    16861 ataacagcgt cgccgggcga ttgtggacca taatccttat aatcagcggc gtcgcggtcc
    16921 tcggttacgc gacgacctcg ctcgtggcgc tggccgtcga gggcacggta cggaactact
    16981 tcaggagcag gcggatgcgg gcggagatca gcaaattgag cggccactac atcctgtgcg
    17041 gatacggccg cgtcggacgt caggtggccg ctgagttcgc tctggacggc gtgcctttcg
    17101 tcgtcataga ccaggacccg aggatcgtcg aggagtgcct ggagagaggc tacctggctc
    17161 tgcccggcga ggcttccgac gacggcacac ttgaggaggc cggcgtcatg cgcgcccgcg
    17221 ggctcgtcgc cgccgtggac tcggacgcgg ataacgtctt cgtcgtactc tcggcccgca
    17281 agctcaaccc ggctttgcac atcgtcgccc gcgcctcctc ggatgggtcg gcggccaagc
    17341 tggagatagc cggcgcggac cgtaccctct cgccatacgc tgtcggcgga cgcaggctcg
    17401 cctcgctcgc cacgcagccg atggtggtgg actttctgga cgtggttacg cggggggagg
    17461 agggaataga gtttcgactg gaggagttca tggtgccaaa ggagccggct atcgcggacc
    17521 acaccatcgg ggagctaaag atcggggagc ggaccggcgc catagtcctc gcgttccgta
    17581 ccgcggacgg caagtttgat acgacgcctt cggcgggcga ccgcctacac gcgggagata
    17641 cgctcatcgt gctcggaagc cgggagcaga tcacgcgcct tgagcaactc atgcgcggcg
    17701 agaagatcac ggagtaacag cgccacctct tcggctggta gtgcttacgg atcgcacgca
    17761 gcatctcctc ggtccagcgt ctcgtcggct agcaccgggc cgtccgagtc ccggacgaac
    17821 tcctgtagac gcgagatgtc ttctctccgg gcgagggcga tgtcccggaa gcccatgtag
    17881 gcgggccggg agaccggacc catgaatgcc acctgaacgg ccaggagcag caacgaggcg
    17941 tagcgttcca ggcagtcggg cgaagcgggc gaggagcgct ccggcgcaca ggacaaggga
    18001 cgcggagaat tcgggtaggt agttcttcac cgtacgagct cgaccttgcc gacgagcagc
    18061 gccgtcacga gcgcgccgag caggtaaggg accacgagcc accgcgactc catccgcgac
    18121 cggaccgcca gcacgaggaa ggcgacgccg cttgcgacca tcacaggcat gaacccgagc
    18181 atcccggaga agctcgaagc gatctggctc cagcggaact cgtttatgtt cgccttgacc
    18241 gtatctaaga agaagctgcc gctggtgatt gcggtgagcg cgccgaggag gtctggcgcg
    18301 gcctaatgga tgtccgcgcg ccgcttcttg cccaagcttc tctcctccgt agtcccactt
    18361 ctatcctatc cgaaaacgcc aagtcggagg acgtggggag gtgcccagga gcatcgccga
    18421 actcggcgag aagatcgaga gccaggaata gggcttactg cgcgcgtcgc tcgtaggcgc
    18481 cgatgtcgca acgcgaaatc ccgtcgctgt ccccgtcctg tgggcggcgt atccctcgct
    18541 gatcggtcgt cggcggcgtg cagtccccgc gcaccgcgtc cacgggcggg gtgcctccca
    18601 ccaaagctat tgtcatggtc gggccgccgt tgtcccggag acccctcggg tcgaggccgg
    18661 acggggtgtt ttgcaggtct gtcgccccga agcccgtcgc gccgctagtg tcgcctatca
    18721 ggttgtagcc tccgctggca aagggaccgg aggcgtccgg gcctgtgggg gcctgattct
    18781 tggcgacgat ggtgtcccgg aggaagattt tgccactatc cccgcttact ggattaataa
    18841 cattgtttat gccacctcca tcgctggagc ttccggagga gacattaccg gaaaaggtgc
    18901 tatccacaat cgatacgcgg ccaagaaagt tctggatgcc acctccaccg ccgatgaaac
    18961 tgtcggcagt tccgagatcg gaattcgcgg aaaaggtgct attcctgact gtcaaattgc
    19021 cctgattgtt accgatgcca cccgcgccag aatcggcctt gttcccggag aaggtgctgc
    19081 tctctaccaa cacctgtcca cggtcgttgg agatgccacc gccgaaaccg gcggagtttc
    19141 cggagacgat gctgttcctg atcaccactg tgccaaggtt ttcgatgcct ccaccaccgc
    19201 tgcgcctgcc g
//
LOCUS       DASSMB010000154         3277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1050026, whole genome shotgun sequence.
ACCESSION   DASSMB010000154 DASSMB010000000
VERSION     DASSMB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050026"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFJ72_09210"
     CDS             complement(<1..317)
                     /locus_tag="VFJ72_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET7479678.1"
                     /translation="MGVIAIGADDAALELKQTLAEHLKELGHEVKDYSPASKEEAESA
                     GVDYPDVAVEVAEAVARGDHDRAILVCGTGIGMSITANKVPGVRAAQCHDVFSAERAR
                     KSN"
     gene            complement(369..1892)
                     /locus_tag="VFJ72_09215"
     CDS             complement(369..1892)
                     /locus_tag="VFJ72_09215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7479679.1"
                     /translation="MATDLVIGIDCSTTATKAVVWDSRGNAVAEGRGTFQLSSPQPDW
                     GEQNAEDWWTSTKEALRIAAQSVDQRRIGAIGITHQRETFVCLNENDQPLRPAMLWLD
                     SRAREEVAKYGSEEVHRLTGKPPNPTPALFKILWMREHEPDILDRTARLVDVHAYLVH
                     KMTGHWRTSWATADPLGLVDMETFEWSDELLNLAGLSREQMCELYAPGDVIGELRSEI
                     AREIGLPDGLPIVSGVGDGQSAGLGANITEPGKAYLNLGTAVVSGTYSENYSWGNEYR
                     VLAGPIPRTYTLETLLAGGTFTISWFVDKFAGFDGSDLGLGLGTEQILESAAAQLKPG
                     SGGLLAVPYWNNALTPYWDTAARGVMLGWTGAHGKAHAYRAILEGIAFEQRLMTDGAE
                     SGLEKPVEHVLALGGGSRSSLWCQIVADIMQRPVSVCQEIESTCLGSGMLAAAASGMH
                     DGIREAAEAMSGEGARYEPDEKRAAFYDRLYNEVYKEIYPSLRPIFPALTDVMKSAE"
     gene            2027..2779
                     /locus_tag="VFJ72_09220"
     CDS             2027..2779
                     /locus_tag="VFJ72_09220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET7479680.1"
                     /translation="MFDGDVAWVTGSSKGIGRAVALDLAREGCDVAVHYNSGEDEARE
                     VVEQIEALGRRAFAVRGDVSDGAEVKRMVREIEDRFGRVDVLVNNAGSFVERRTLEEM
                     TEDVWDRVMAVNLKSVYLCSQAVLHLMRRQGGGKIVNISSVSARIGGSPDSIAYSTAK
                     GGVSTLTRAMAKALLPDNILVNAVAPGRIDTPLHDRFTPAEKREEGARKVPLGREGTP
                     EEVSGAVVYLASDRASYLVGEVIEVNGGLWMG"
     gene            2842..>3277
                     /locus_tag="VFJ72_09225"
     CDS             2842..>3277
                     /locus_tag="VFJ72_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7479681.1"
                     /translation="MKEDRVKYRELREEVARVARALISSGLVTGTSGNVSARTPEGDV
                     LITPSGLDYEILRPDDVVLVDLDMNVLEGSLVPSTETPMHTGIYRARRNVEAVVHTHS
                     RFATTLACLGWEIPPVHYMLTTLGPDGRIPLAPYVLYGTEQLA"
BASE COUNT          500 a         1115 c         1079 g          583 t
ORIGIN      
        1 ttgttggact tcctggcccg ctcggcggag aagacgtcgt ggcactgggc tgcccgcacg
       61 ccgggcacct tgttcgccgt gatgctcatg ccgatccccg taccgcacac caggatggcc
      121 cggtcgtgat ctcccctggc gaccgcctcc gcgacctcca ccgccacgtc cgggtagtcc
      181 acgcccgcgc tctcggcctc ttccttggag gcggggctgt agtccttcac ctcatggccc
      241 agttccttga ggtgctcggc cagcgtctgc ttgagttcca gcgccgcgtc gtcggcgccg
      301 atggctatga ctcccatctt tcgacctcct gatggctaga tctacggatc ttgtatatct
      361 tactccgctt actccgccga cttcatcacg tcggtcaggg ccgggaagat ggggcggagg
      421 ctcgggtaga tctccttgta aacttcgttg tagaggcggt cgtagaacgc ggcccgcttc
      481 tcgtcgggct cgtagcgggc gccctcgccg ctcatggctt ctgcggcctc gcggatgccg
      541 tcgtgcatgc cggacgcggc ggcggcgagc atcccggagc cgaggcaggt gctctctatc
      601 tcctggcaca ccgagaccgg acgctgcatg atgtccgcca cgatctggca ccacagcgag
      661 ctgcgcgatc cgccgcccag ggccaggacg tgctcgaccg gcttctccag gccgctctcc
      721 gctccgtcgg tcatcagccg ctgctcgaag gctatgccct ccaggatcgc gcggtaggcg
      781 tgcgccttgc cgtgggcgcc ggtccagccg agcatcaccc cgcgcgccgc cgtgtcccag
      841 tacggtgtca gggcgttgtt ccagtacggg accgcgagca atcctccgga gccgggctta
      901 agctgcgccg cggcggactc caggatctgc tccgtcccca gcccgagccc cagatcgctg
      961 ccgtcaaagc cggcgaactt gtccacgaac cagctaatag tgaacgtgcc gcccgcgagc
     1021 agggtctcca gggtgtaggt gcgcgggatc gggccggcga gcacccggta ctcgttgccc
     1081 caggagtagt tctcgctgta cgtaccggag acgactgccg tgcccaggtt gaggtacgcc
     1141 ttccccggct ccgtgatgtt cgcccccagc ccggccgact gcccgtcgcc gacgccgctc
     1201 acaatgggga gtccgtcggg aagcccgatc tctcgcgcga tctcgctcct aagctccccg
     1261 ataacgtcgc ccggagcgta cagctcgcac atctgctcgc ggctcaggcc cgcgaggttc
     1321 agcagctcgt cggaccactc gaaggtctcc atgtccacga ggcccagggg gtcggcggtg
     1381 gcccaggagg tgcgccagtg tccggtcatc ttatgcacca ggtaggcgtg tacgtccacc
     1441 agacgggccg tccggtccag gatgtccggc tcgtgctcgc gcatccacag gatcttgaac
     1501 agggccggcg tcgggttcgg cggcttgccg gtgagccggt gtacttcctc ggagccgtat
     1561 ttggcgacct cctcgcgcgc ccgcgagtcg agccacagca tcgcgggccg caggggctgg
     1621 tcgttctcgt tcaggcacac gaacgtctcg cgctgatggg tgatgccgat ggccccgatc
     1681 ctgcgctggt ccacgctctg agccgcgatc ctcagcgcct ccttcgtcga ggtccaccag
     1741 tcctcggcgt tctgctcgcc ccagtccggc tgcgggctgg agagctgaaa agtcccccgg
     1801 ccttcggcca ccgcgttccc ccggctgtcc cacaccacgg ccttcgtcgc cgtcgtcgag
     1861 cagtcgatcc ctatgaccag atcggtcgcc atgcgcgccc cttcctattg gccttgtccc
     1921 tgcgcgacat catagaacat ccgcattttt cgcgttaccg cttctccgtt tgcctctgac
     1981 ggagcgagaa gataggctag gtacctgcga acgggaagga gccggattgt tcgacggaga
     2041 tgtggcgtgg gttaccggca gcagcaaggg gatcgggcgc gcggtcgccc tcgacctggc
     2101 ccgcgagggt tgcgatgtcg ccgtccacta taacagcggc gaggatgagg cccgggaggt
     2161 ggtcgaacag atagaagccc tggggcggcg ggccttcgcg gtgcgcggag acgtctccga
     2221 cggcgcggag gtgaagcgga tggtccggga gatagaggac cgcttcggcc gcgtggacgt
     2281 gctggtgaac aacgcgggtt ctttcgtcga gcggcgcacg ctggaggaga tgaccgagga
     2341 cgtgtgggac cgggtgatgg cggtcaacct caagagcgta tacctctgct cgcaggcggt
     2401 actgcacctg atgagacgtc agggaggcgg aaagatcgtc aatatctcct ccgtctcggc
     2461 caggatcggc ggcagccccg actccatcgc ctactctacc gccaagggtg gcgtcagcac
     2521 cctgacccgc gcgatggcga aagccctgct ccccgacaac atcctggtca acgccgtcgc
     2581 ccccggccgc atagacaccc cgctccacga caggtttacc ccggccgaga aacgggagga
     2641 gggcgccagg aaggtccctc tcgggaggga aggcacgccc gaggaagtct ccggtgccgt
     2701 ggtgtacctc gcgtccgacc gcgcgagcta cctcgtcggc gaggtcatcg aggtgaacgg
     2761 cgggctgtgg atgggctgat ggatcgtggc gtcgcgatgc tccggcatcc gccgccaggg
     2821 tgttgagttt gaggttcagt agtgaaggag gatagggtga agtaccggga gttgcgcgaa
     2881 gaggtggcgc gggtggcgag ggctttgatc tcctcgggtc tcgtcaccgg cacgtcgggg
     2941 aacgtcagcg cccgcacccc ggagggtgat gtcctgatca cgccgagcgg cctggactac
     3001 gagatcctac ggcccgacga cgtggtgctc gtggacctgg acatgaacgt cctggagggc
     3061 tccctggttc cctccacgga gaccccgatg cacaccggca tctacagggc gcgccggaac
     3121 gtggaggccg tggtccacac ccactcccgc ttcgccacca ccctggcgtg cctcggttgg
     3181 gagataccac cggtccacta catgctaacc accctcggcc cggacggccg catcccgctg
     3241 gcgccatacg tgctctacgg caccgagcaa ctggccg
//
LOCUS       DASSMB010000155         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_922174, whole genome shotgun sequence.
ACCESSION   DASSMB010000155 DASSMB010000000
VERSION     DASSMB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922174"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1021
                     /gene="pdxS"
                     /locus_tag="VFJ72_09230"
     CDS             125..1021
                     /gene="pdxS"
                     /locus_tag="VFJ72_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET7479682.1"
                     /translation="MAENGQHTGTFRVKSGMAQMLKGGVIMDVVNAEQAKIAEEAGAV
                     AVMALERVPADIRADGGVARMSDPEMISEIQQAVSIPVMAKARIGHFVEAQVLEALEV
                     DYIDESEVLTPADEAHHIDKAAFEVPFVCGATNLGEALRRIGEGAAMIRSKGEAGTGN
                     VVEAVRHMRAIVGGIRKLGVLEHEELMSEAKNLQAPYELVRWVAENGRLPVVLFTAGG
                     IATPADAALMMQLGADGVFVGSGIFKSGDPAKRARAIVQATTHYKDAKVVAEVSRGLG
                     EAMVGREMGELKEGERLATRGW"
     gene            1011..1610
                     /gene="pdxT"
                     /locus_tag="VFJ72_09235"
     CDS             1011..1610
                     /gene="pdxT"
                     /locus_tag="VFJ72_09235"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET7479683.1"
                     /translation="MAGNGERNGVARIGVLALQGDFREHAEILTKLGVEPVEVRALED
                     LSGLAGLIVPGGESTTIGKMMVSSGLLDGIRSFFYKGGPVWGTCAGMVLAASATTGER
                     QPLLGLMNALVERNGFGRQVHSFETELDVEGFEEPFTGVFIRAPFFEDVGPGVEVLSE
                     VGGRVVAARGENILVTAFHPELTDDVRFHEYFLREVCGL"
     gene            1607..2359
                     /locus_tag="VFJ72_09240"
     CDS             1607..2359
                     /locus_tag="VFJ72_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7479684.1"
                     /translation="MSGHSKWSTIKRKKGAADAKRGALFGKLSKAITVAAREGGGDTE
                     MNPALGLAVQKAKAANMPNDNIQRAIDKGTGAGADAETYERITYEGYAPGGVAVLVDV
                     LTDNRNRAASDVRYIFSKNGGKLGTSGSVAYLFERKGVILVPKDGADEDELMELALEA
                     GAEDVEEQENDYRVVTAPEEFAAVREALDGAGIAYENAEITLEAQNSIDLDAGTAKQT
                     LRLIDALEENDDVQEVYANFDISDEVMAEVVS"
     gene            2441..>2818
                     /locus_tag="VFJ72_09245"
     CDS             2441..>2818
                     /locus_tag="VFJ72_09245"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7479685.1"
                     /translation="MSGRDSTRRFSGRVENYSRYRPGYPRQMLKLLRERCGLSGASVI
                     ADVGSGTGILSRLFLGNGNRVFGVEPNDEMRAAGERSLRGYESFTSVAATAEATTMED
                     HSVDFVAAGQAFHWFEPGRARTEF"
BASE COUNT          521 a          762 c         1091 g          444 t
ORIGIN      
        1 gtacgcgaaa gagcgtaaca agcgctagga agctgctttt agcgggcatg gcaatttggc
       61 gagcaggtgc gccggtagta agatacgtct tacatattct agtaagcatt tacgggagaa
      121 aagcatggcc gagaacggac aacacaccgg gaccttcagg gtcaagagcg ggatggcgca
      181 gatgctcaag ggcggcgtca tcatggacgt ggtcaacgcc gagcaggcga agatagccga
      241 ggaggccggc gccgtcgccg tgatggccct tgagcgggtc ccggccgaca tccgcgccga
      301 cggaggcgtc gccaggatga gcgacccgga gatgatctcc gagatacagc aggccgtctc
      361 tatccccgtg atggcgaagg ctagaatcgg ccacttcgtc gaggcccagg tcctcgaagc
      421 cctggaggtg gactacatag acgagtccga ggtcctgact cctgccgatg aggcgcacca
      481 catagacaag gccgccttcg aggtgccgtt cgtgtgcggc gcgaccaacc tcggtgaagc
      541 cttgaggcgt attggcgagg gtgcggccat gatccgctcc aagggagagg ccggtaccgg
      601 aaacgtcgtc gaggccgtcc gtcacatgcg ggcgatagtc ggaggaatcc gcaagctcgg
      661 cgtgctggag cacgaggagc tgatgagcga ggccaagaac ctccaggccc cttatgagtt
      721 ggtgcggtgg gtggcggaga acgggaggct gccggtcgtg ctgttcaccg ccggaggtat
      781 cgcgacgcct gctgatgctg ccttgatgat gcagctagga gctgatgggg tgttcgtggg
      841 aagcggcatc ttcaagagcg gcgacccggc gaagagggcg cgggccatcg tgcaggctac
      901 cacgcactac aaggacgcga aggtcgtggc tgaggtgagc cgggggcttg gagaggcgat
      961 ggttggcagg gagatgggag agttgaagga aggagagcgg ctcgctaccc gtggctggta
     1021 acggcgagag aaacggcgtg gcgaggatcg gggtgctcgc gctccaggga gacttccggg
     1081 agcacgcgga gatcctgacg aagctcggcg tcgagccggt ggaggtgcgg gcgctggagg
     1141 acctgtcggg gcttgcgggg cttatagtgc cgggcgggga gtccacgacc atcgggaaga
     1201 tgatggtctc ctcggggctc cttgacggca tccgcagctt cttctacaag ggcgggccgg
     1261 tgtgggggac gtgcgcgggg atggtactcg cggcctcggc gactacggga gagcggcagc
     1321 cgctcctggg gctgatgaac gcgctggtcg aacgcaacgg gttcggacgg caggtccatt
     1381 ccttcgagac agagctggac gtggaggggt tcgaggagcc gttcacgggg gtatttatcc
     1441 gggcgccgtt cttcgaggac gtcgggccgg gcgtggaggt gttgagcgag gtcggcggcc
     1501 gggtggtggc ggcgcggggc gagaacatcc tggtgacggc gttccatccc gagcttaccg
     1561 atgatgtgag gtttcacgag tattttctgc gggaggtatg tggtttatga gcggtcatag
     1621 caagtggtcc acgatcaagc gtaagaaggg cgcggcggac gcgaagcgcg gggcgctctt
     1681 cgggaagctc tcgaaggcta ttacggtggc cgcgcgcgag ggcggcggcg acacggagat
     1741 gaacccggcg ctggggctcg cggtgcagaa ggcgaaggcc gccaatatgc ccaacgacaa
     1801 catccagcgg gccatagaca agggcacggg cgcgggggcc gacgccgaga cctacgagag
     1861 gatcacctac gagggctacg ctcccggcgg cgtcgcggtc ctggtggacg tgctgaccga
     1921 taaccgcaac cgggcggcct cggacgtgcg ctacatcttc tccaagaacg gcggcaagct
     1981 cgggacgagc ggctcggtgg cgtacctctt cgagcggaag ggcgtcatcc tggtcccgaa
     2041 ggacggcgcg gacgaggacg agttgatgga actggctctc gaagcggggg ccgaggacgt
     2101 ggaggagcag gagaacgact accgggtagt cacggcgccc gaggagttcg cggcggtgcg
     2161 cgaggcgctg gacggcgcgg gcatcgccta cgagaacgcc gagatcacgc tggaggccca
     2221 gaacagcatt gacctcgacg ccgggaccgc gaagcagacc ctgcgcctca ttgacgccct
     2281 ggaagagaac gacgacgtgc aggaggtcta cgccaacttc gacatcagcg acgaggtcat
     2341 ggcggaggtc gtctcctagc cgaccggcct tcggtggcgg atctatcgtg tttggggcgg
     2401 ccggcacacg ccggccgctt cgtcgtggac gggggtggct gtgagcggta gggactcgac
     2461 gcggcggttc tcgggcaggg tggagaacta ctcccggtac cggccgggct acccgcgcca
     2521 gatgttgaag ctactgcgag aaagatgcgg gctctcgggt gcctcggtga tcgcggacgt
     2581 ggggtccggg acggggatct tgtcccggct gttcctcggg aacggcaacc gggttttcgg
     2641 ggtggagccg aacgatgaga tgcgggccgc cggcgagcgg tccttgcggg ggtacgaatc
     2701 ctttacgagc gtcgcggcca ccgcggaggc tacgacgatg gaggaccaca gcgtcgactt
     2761 cgtggcagcg ggccaggcgt tccactggtt cgagcctggc cgggcgcgga cagagttc
//
LOCUS       DASSMB010000156        13103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_289600, whole genome shotgun sequence.
ACCESSION   DASSMB010000156 DASSMB010000000
VERSION     DASSMB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13103
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289600"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1005
                     /locus_tag="VFJ72_09250"
     CDS             73..1005
                     /locus_tag="VFJ72_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET7479686.1"
                     /translation="MSPKPVILDVDPGHDDAVALMMACGAPGLDLRAVTTVAGNVSLE
                     KTTRNALRVLSLIGRADVPVAAGAAQPLERPLRTATDIHGESGLDGPEEVPDATFEAD
                     GRGAVDLIADTLRESPEPVTLVPVGPLTNIALFLREHPELKHRIERISLMGGSIGLGN
                     TTPAAEFNIYVDPEAAREVFDSGLPITMSGLDVTHQAGAGPKERERLRSLGEVGGVVA
                     GFLEFFAATYESIYGFDAPPLHDPVAVAAVLDPTLLKTRLMNVDVECEGELTRGETVC
                     DFYGVTGKLANADVGIELDREGFFELLYESLGNL"
     gene            1052..1657
                     /locus_tag="VFJ72_09255"
     CDS             1052..1657
                     /locus_tag="VFJ72_09255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7479687.1"
                     /translation="MRKVYEARNDRELAEGYDAWAGEYEGDVSSFGYKIPAVVTGLAG
                     RYLDRGGRLLDAGAGTGVLGEILHLLGYTDLTGIDLSEGMLEKAREKGIYGSLRRMAL
                     GERLDFLDGAFDATLSSGVFTTGHAPPESLREISRVTRPGGHVIFSVRETVYLEGGFG
                     EEQESLEREGRWRLVEQTGTFQSLPLGEPEVLGRVFVYEVS"
     gene            1698..3260
                     /locus_tag="VFJ72_09260"
     CDS             1698..3260
                     /locus_tag="VFJ72_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HET7479688.1"
                     /translation="MRERDTTPVPPSGPDPFRSWLEGARLWNEGAFGARRVLTKAARR
                     SSEDTRAVLDAASAANGTPDLATLARLGDLSRAQWFLWTGAGLAAGERAGRATSAKGY
                     LPGAYSPRALVLGMVASDLYLGYVALRERARWFPDLVTDEDWKLQHRRGAGRALDAAE
                     ALGGTLIKAGQFASSRPDLLPAAYTETLSSLQDRVPPQPFAVIEEAVVRELGRPIAEV
                     FEEFDEEPIAAASIAQVHRARLRGDREVAVKVQYPGIAGLIEADLDALESIFEAVARL
                     EPGIRLKPIADYLRWTLPLELDFRREARSIGELGDALADRDDVIVPGVVGGISTGRLL
                     VMELVRGVKITDTDALVAAEIDPGEVATLLNDVYADQLFKRGILHADPHPGNLLVQPG
                     PRLVLLDHGLTLPLERSFISALEKMVSALGEGDLDALIPALREAGLPVSEDTDLDTLL
                     QVVGVLLGGEREETSASFEDFGRRLGASVGDIPPKLLLVGRAIGLLDGITRQLDPEAD
                     ALEIVARYTGNP"
     gene            complement(3282..3692)
                     /locus_tag="VFJ72_09265"
     CDS             complement(3282..3692)
                     /locus_tag="VFJ72_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7479689.1"
                     /translation="MADIKKMTIQHLGDQRYAGRNDGGDRILIDGSDDTTVGVRPMEA
                     LLAALGACTAFDVVSILAKRRTPVESYRIELEGERADDHPRRYTRILVRHVVSGDGIT
                     PESLERAVALSHEKYCSVAATLNSEIESEAVLEE"
     gene            3860..4267
                     /locus_tag="VFJ72_09270"
                     /pseudo
     CDS             3860..4267
                     /locus_tag="VFJ72_09270"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
     gene            4358..5137
                     /locus_tag="VFJ72_09275"
     CDS             4358..5137
                     /locus_tag="VFJ72_09275"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7479690.1"
                     /translation="MDAEGRGDRFQKLEESYKDYTVYDQHYEKIGKVDDLFVDNEDQP
                     EYIGVKTGFLGTKSTLIPMEIARVNDRRKLIEVAADREAIKDAPTFDNDEEITPEREH
                     RIHSYFGLERPESWNERGGYGDYYHSDTEDHAREDLTGSVDTEYGERRGESSDRPIET
                     APSREERYDTESSRSGASDRGDLDIPLDDTEHGTERNTGPERPRGYTDSTEPREDTAE
                     PERDPIGGDATARTEREDQTFTDEGESRGMRIHKRVRTRSE"
     gene            5263..6543
                     /gene="larA"
                     /locus_tag="VFJ72_09280"
     CDS             5263..6543
                     /gene="larA"
                     /locus_tag="VFJ72_09280"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HET7479691.1"
                     /translation="MQTQLQYGKGDLSVEIPSSDVTVLAPKHEDGLPDEAAAFRESVR
                     NPIGSAPLGEVVGATEKVAVVIPDITRALPRERLLPWLFEELSHVPPENVTIINGTGS
                     HRTNTEDELAAMVGREVYERYRVINHNAHDPETLAFAGKSPEGRDVYMNREYVAADRR
                     IVMGFIEPHFMAGFSGGYKGIFPAVADIDSIMHYHRAAVIGHERSTWGVMEDNPTQEQ
                     IRANGSLLPVDFCINVTLNRAREITGFYCGDALTAHLEGCEASKASAMVACEREYPIV
                     VTTNGGYPLDQNLYQAVKGMSAAYQIVSDGGLIISVARCNDGFPEHGNFRKMLLEHDS
                     PEAMLETIYTPGFSLYDQWQVQLFAQILKKARVGLHSEIDPEAVRSVHLEPVADITAR
                     ISEELESIGSDAPIAVLPEGPMTVPYLEHMPVGA"
     gene            complement(6554..7516)
                     /locus_tag="VFJ72_09285"
     CDS             complement(6554..7516)
                     /locus_tag="VFJ72_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479692.1"
                     /translation="MAERIDLVYPLYLDVPMMTSFVAALEDGIAYGSDVTQRQDQQRK
                     LGAGSEARVGVPGMGIFSTLLNIDLRGKITGEQVSGSDEEIKLVRRHTEASLFMRLRH
                     TLINDNRILQINNIDDIQQLKDAEQDYLVEIRGQISRSPLNESLEAVFRAMAMLGVET
                     PTNQTLRTQTGGGKKNRGQGQQNASSNNPFALDREAMMGLEIMQRIREDLAQSKILDA
                     VMRPSTADELTVVIALALEFLPEGAFDNLLSGDFSVLGKVTRLVEGADEISLYQRTAF
                     RNLEFGSLDAVFSEMRKGGIFKLSDSPSSIKAPAIQIMPMAIYA"
     gene            7808..8098
                     /locus_tag="VFJ72_09290"
     CDS             7808..8098
                     /locus_tag="VFJ72_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET7479693.1"
                     /translation="MVRSFDKVRRINISRAILTGKGQLTVPKDVRERLGLKRGDRVVF
                     EFEGDSVRLKVEERKTLKELKGSLPATREYPGKDAERQAAREHVIREKLGGS"
     gene            8095..8511
                     /locus_tag="VFJ72_09295"
     CDS             8095..8511
                     /locus_tag="VFJ72_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7479694.1"
                     /translation="MSQEVSSWWLDANVLLRFLTGDPPELAERAAGLLERAQRGEVLL
                     RVHAVVVAETVWVLESFYEYSKEDTSGALIPLLEQPALRVEGARTVVRALEAMSQNNV
                     DFADALLAETARSRGEGVASFDRDFRKLGIEWQEPD"
     gene            complement(8555..9661)
                     /locus_tag="VFJ72_09300"
     CDS             complement(8555..9661)
                     /locus_tag="VFJ72_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479695.1"
                     /translation="MADDGLFQMDLNLHPNEASGLLSIGDEALLKRHGWDIGFWAVLD
                     EGKVEDCIAAIGHRSDAPEDDGWEIERLRARVSGDAGKTEDAEAITRDESTGFVYILG
                     SHFGGKAGPLQPKRGFVARFREAGVGHVTEDPAMEIVISRRSFALHRLINDALRSSGP
                     DLIPLGPESHEALIAATIQRGEQENKRWSGLVREDDYPINIEGAAFRESGTLLLGLRF
                     PTAADGRPILIELEGIDRLFEPGDELPEVRGFWTMDAVGNNGEIAGVRDLALLQTGED
                     EQLHLVTGNVDSRDKQSVLVQDYPGGRETVATHFCCALPPDAHSGTLEAEFVREFPAL
                     PRVEGIAVTHEGRAYYVTDEDEGVHLRLTRLLAG"
     gene            complement(9761..10126)
                     /locus_tag="VFJ72_09305"
     CDS             complement(9761..10126)
                     /locus_tag="VFJ72_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7479696.1"
                     /translation="MVTRASGGLDIGELRRAIEERDLERMLSFYDDDAEILTVDRNAP
                     PSAPHELRGKAAITEYLKDIFGREMTHRVENEVVGEDRIAFAERCEYPDGTKVLALET
                     CELRDGKILRQVGVQVWDE"
     gene            10391..11215
                     /locus_tag="VFJ72_09310"
     CDS             10391..11215
                     /locus_tag="VFJ72_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET7479697.1"
                     /translation="MALTVGSGPFSERHEGTFNFEVDAPKGHVLFLEDSPRRVRVMFG
                     GETVADSRRVKLLHETGHLPVYYFPEEDVRMDLLEATDHSTHCPFKGDASYWSVKAGG
                     KVAENAVWGYPETLEGCPPISGYLAFYWDAMDSWLEEEEEVFVHPRDPYHRVDILESS
                     RHVKVTVNGEVVAETERPKVLFETALPPRYYIPPDDVRTDLLTPTEKHTGCPYKGVAS
                     YWSVEAGGEVAEDLVWSYPEPIAAVEKIRDHLCFFNEKVDLEVDGEPQPRPETQWS"
     gene            complement(11241..11657)
                     /locus_tag="VFJ72_09315"
     CDS             complement(11241..11657)
                     /locus_tag="VFJ72_09315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET7479698.1"
                     /translation="MLDTTSEAGRRAERRLLEEEIVWFTTVREDGQPQSVPVWFLWDG
                     ERFLIYSQQGRRKLRNIARNPRVGLNLNSNALGGDVVRAEGAAEIVEDAPPVSEISAY
                     IEKYRESIARIGFDVEGFTRAYSIALQVTPTRWQVW"
     gene            complement(11769..12350)
                     /locus_tag="VFJ72_09320"
     CDS             complement(11769..12350)
                     /locus_tag="VFJ72_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7479699.1"
                     /translation="MWIFLETERMLLRRFTGADVDNLVDLDGDPDVMRFINGGRPTSR
                     GVIEDETLPRFLRYYERFKGFGFWAAIEKSTGEFLGWFHFRPPEGRTANEVELGYRLR
                     RSAWGKGYATEGSRALIRKGFTELGVRRVVAETMAVNAASRRVMEKSGLTLARTFHGE
                     WPEPIEGAERGEVEYAVTRADWERREAGGLRAT"
     gene            12579..12995
                     /locus_tag="VFJ72_09325"
     CDS             12579..12995
                     /locus_tag="VFJ72_09325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7479700.1"
                     /translation="MVLGLMLLGAIFLLWNADHERVREVSGIRHSAVTGAPQAPSGNG
                     GEGKMMDASYYGHELEGSPTASGQPFDPEKYTAASKTLPLGTKLKVSHGGESVRVTVN
                     DRGPYVPGRELDLSLAAARKIGLTGQAEAPVRVVVL"
BASE COUNT         2396 a         4187 c         4172 g         2348 t
ORIGIN      
        1 cggccgtgct gtacgcggcg acgaaaaaga gataacttgc gtacagagtc cgcggcatgg
       61 agaggaacgc gaatgagccc gaaaccggtg atcctggacg tggaccctgg ccacgacgac
      121 gccgtggccc tgatgatggc ctgcggagcg ccgggcctgg acctccgcgc cgtcaccacc
      181 gtggccggga acgtctccct ggagaagacc acccgcaacg ccctgcgcgt cctctcgctc
      241 atcgggcggg cggacgtgcc cgtggcggct ggggccgcgc agccgctgga gcgcccgctg
      301 cggaccgcca cggacatcca cggcgagagc gggctggacg ggccggagga ggtcccggat
      361 gcgaccttcg aggccgacgg gcgcggcgcg gtagacctca tcgccgatac gctgcgggag
      421 tcgccggaac cagtcaccct cgtcccggtc ggcccgctca ccaacatcgc tctcttcctg
      481 cgcgagcacc cggaactgaa gcataggatc gagcgcatct cgctgatggg cggctccatc
      541 gggctcggca acacgacgcc cgccgccgag ttcaacatct acgtggaccc ggaggccgcg
      601 cgcgaggtct tcgactccgg cctgcccatc acgatgtcgg gcctcgacgt gacgcatcag
      661 gccggagccg gcccaaaaga gagggagcgg ctgcgctcgc tgggcgaggt cgggggcgtg
      721 gtggcggggt tcctggagtt cttcgccgcc acctacgaga gcatctacgg cttcgacgcg
      781 ccgcccctgc acgacccggt cgcggttgcg gcggtgctcg atcctactct tctaaagacg
      841 cgcctgatga acgtggacgt ggagtgcgag ggcgagctga cccgcggcga gaccgtctgc
      901 gacttctacg gcgtaaccgg aaagctcgcc aatgccgacg tgggtatcga gctggaccgc
      961 gagggtttct tcgagctgct gtacgagtcg ctgggcaacc tgtaaacata cgaaacggaa
     1021 ggtgatctcc atctccgaca gatccgagag gttgaggaag gtttacgagg cccggaacga
     1081 ccgggagttg gccgagggtt atgacgcctg ggcgggtgag tacgaggggg acgtgtcgag
     1141 cttcgggtac aagatccccg ccgtcgtgac cggtctggcc ggacgttacc tggaccgcgg
     1201 cggcaggctg ctcgacgccg gagccggaac gggggtcctc ggcgagatcc tgcacctcct
     1261 tggctacacg gacctgaccg ggatcgacct ctcggaaggc atgctggaga aggcccgcga
     1321 gaagggcatc tacgggtccc tgcgccggat ggcgctgggc gagcgcctgg acttcctcga
     1381 tggagccttc gacgccactc tatcctcggg cgtcttcacg acgggccacg ccccgccgga
     1441 gtcgctgcgc gagatctcgc gcgtcacccg tcccggcggg cacgtcatct tcagcgtccg
     1501 ggagaccgtg tacctcgaag ggggattcgg ggaagagcag gagtctctgg agcgggaagg
     1561 ccggtggcgg ctggtggagc agaccggtac cttccagtca ctgcccctgg gcgagcccga
     1621 ggtcctcgga cgcgtgttcg tatacgaggt ctcctgatcc cccactccgc tagatccatc
     1681 gcgtatactc catcgctatg cgtgaacgag atacgacgcc ggtgcccccc tccggcccgg
     1741 accccttccg tagctggctg gagggcgccc gcctgtggaa tgagggtgcg ttcggagccc
     1801 ggcgcgttct gacgaaggcc gcccggcgct cgtccgagga tacccgggcc gtcctggacg
     1861 ccgcgtccgc cgcgaacggt acgccggacc tcgccaccct ggcccgcctg ggagaccttt
     1921 cgcgagcgca atggtttctg tggacgggcg cgggactcgc ggcgggcgaa cgcgcgggcc
     1981 gcgccacgtc ggcgaagggc tatctccccg gcgcgtactc gccgcgggcg ctggtgctcg
     2041 ggatggttgc ttcggacctg tatttaggct acgtggcgct gcgtgagcgg gcgcggtggt
     2101 tccccgacct cgtcacggac gaggactgga agctgcaaca ccggcgcggt gcggggcgcg
     2161 cgctggacgc ggcggaggcg ctgggcggca cgctcataaa ggccgggcag ttcgcctcgt
     2221 cgcggccgga cctcttgccg gccgcgtaca ccgagacgct ctcgtcatta caagaccggg
     2281 tgccgccgca gccgttcgcg gtcatcgagg aggctgtggt ccgcgagtta ggacgtccca
     2341 tcgcggaggt cttcgaggag ttcgacgagg agccgatagc cgccgcctcc atcgcgcagg
     2401 tgcatcgcgc gcggctgcgc ggcgaccggg aggtggcggt gaaggtccaa tatcccggca
     2461 tagcgggcct tatcgaagcg gatctcgacg ctctggagag catcttcgag gcggtagcgc
     2521 gcctggagcc cggcatccgg ctaaagccca tcgccgacta cctgcgctgg acgctgccgc
     2581 tggagctgga cttccggcgc gaggcgcggt ccataggaga actcggggac gctctggcgg
     2641 atcgggacga cgtgatcgta cccggagttg ttggtgggat cagtaccggg cggttgctgg
     2701 tgatggaact cgtcaggggc gtcaagatca cggacaccga cgctctcgtg gcggcggaga
     2761 tcgaccccgg cgaggtcgcc acgctcctga acgacgtcta cgcggaccag ctcttcaagc
     2821 gcggcatcct gcacgccgac cctcatcccg gcaacctgct cgtccagccc ggcccgcgcc
     2881 tagttctcct ggaccacggc ctcaccctcc ccctggagcg ttcctttatc tccgctctcg
     2941 aaaagatggt ctccgccctc ggggagggcg acctcgacgc gctcataccg gcgctgcgcg
     3001 aagcgggcct gccggtgagc gaggacaccg acctggatac gctgctacag gtggtcgggg
     3061 tgctgctcgg cggagagcgg gaggagacca gcgcgagctt cgaggacttc ggccgcaggc
     3121 tcggcgcgag cgtcggggac ataccgccga agctcttgct agtggggcgg gctatcgggc
     3181 tgctcgacgg cataacccgc cagctagacc cggaggcgga cgccctggag atcgtagccc
     3241 gctataccgg gaatccgtag cccgaccgga tagcgctcca gctactcttc caggaccgcc
     3301 tcggactcta tctcggagtt gagggtggcg gcgacggagc agtacttctc gtgcgagagc
     3361 gcgacggccc gctccaggct ctcgggcgtg atgccgtcgc cgctgacgac gtggcggacc
     3421 agtatcctcg tgtacctccg cggatggtcg tcggctcgct cgccctccag ctcaatacgg
     3481 tagctctcca ccggcgtgcg acgcttcgcg aggatggaga ccacgtcgaa agcggtgcag
     3541 gcgccgaggg ccgcgagcag agcctccatc ggtcggacgc cgacggtcgt atcatcgctg
     3601 ccgtcaatga ggatccggtc tccaccgtcg ttgcgccccg cgtaacgctg gtctccgagg
     3661 tgctggatgg tcatcttctt gatgtccgcc atgccggtac tctacatcag cggcagtcca
     3721 gacgaacgag cctctcgtct cttcccgaag aacacaatcg cgccgtctcg cgtacactta
     3781 cggtccgcac gaatacgtta cgaaaggact tctcttgacg gagctaccac agcagggcca
     3841 gtgactccca aagaccgagt tcgtcaccga gagcggcgac cgtctctcct ccacggacct
     3901 cgccggggag aagaccgtcc tctacttcta ccccaaggac gacacccccg gctgcaccaa
     3961 ggaagcgtgc gccttccggg accggatggg agaatacgaa gccgccaaca taaaggtcta
     4021 cggcgtctcg ctcgattccc cggagtcgca ccgcgagttc cgcgagaagc acggtctcaa
     4081 cttcccgctc ctcaccgacg agggcggtcg cgcgtccgag gcgctcggag tgctgcgcga
     4141 gaaccggcag gtggcgaacc gcgtcacgtt cctgctcgat ccggaaggca ggatctccag
     4201 ggtctatccc gaagtctcgc cagagaccca cgccgacgag atcctctcgg acgcggcgtc
     4261 ggtataggag gcttcaggag gtcccgacga gcgtttcggg ccatcagagc cagggtaagc
     4321 acgaccgtgc cataaacggg ccagggcagg aggaaaagtg gacgccgagg gacgcggaga
     4381 tcgttttcag aagctagaag aatcgtacaa ggactacaca gtctacgacc agcactacga
     4441 gaagatcggt aaagtagacg acctcttcgt ggacaacgaa gaccagcccg agtacatcgg
     4501 cgtcaagaca ggtttcctgg ggacgaaatc caccctgata ccgatggaga tcgcccgcgt
     4561 caacgaccgg cggaagctaa tagaagtagc ggcggataga gaagcgatca aggacgcccc
     4621 cactttcgac aatgacgagg agataacgcc cgaacgagag caccgcatcc actcctactt
     4681 cggcctggag cgccccgagt cctggaacga gcgcggcggc tacggcgact actaccactc
     4741 tgatacggaa gaccatgccc gcgaggacct gacgggctcc gtggacaccg agtacggaga
     4801 gcgaaggggc gaatcgtcag accgtccgat tgagaccgcg ccctcccgcg aagaacgcta
     4861 cgacaccgag tcctcgcgct ccggcgcatc ggaccggggc gacctggaca taccgctgga
     4921 cgatacagag cacggtacgg agcgcaacac gggccccgaa agaccccgag gctatacgga
     4981 ttctacggag ccaagagaag acacggcgga gccggagaga gaccccattg gcggcgacgc
     5041 tacggcgagg acggagcgag aggatcagac cttcaccgac gagggggagt ccaggggcat
     5101 gcggatacac aagcgagtcc gtacgcgcag cgagtagaac cgccgtaaga agcacgacct
     5161 cggctctccg ctgctcacca ctcgaagtac atgctcgttg atgagatccc ggacggcgcg
     5221 gcctcgttgc agaatgaagc aaaggtaggg aggtttcgta cggtgcagac gcaactgcaa
     5281 tacggcaagg gcgacctgag cgtggagatc ccatcttcgg acgtgaccgt tctggcgccg
     5341 aaacacgagg acgggctgcc cgatgaggcg gcggccttcc gggagtcggt gcggaaccct
     5401 atcggctcgg cgccgctcgg ggaggtcgtc ggcgcgacgg agaaggtggc cgtcgtgatc
     5461 ccggacatca cccgagccct gccccgcgaa cgtctcctcc cctggctctt cgaggagctc
     5521 tcgcacgtcc cgcccgagaa cgtcaccatc atcaacggta ccggctccca ccgcaccaac
     5581 accgaggacg agctggccgc gatggtcggg cgcgaggtgt acgagcgcta ccgggtcatc
     5641 aaccacaacg cccacgaccc ggagacgctg gccttcgccg gcaagtcgcc cgagggtcgg
     5701 gatgtgtaca tgaaccgcga gtacgtcgcg gcggaccggc gcatcgtgat ggggtttata
     5761 gagccgcact tcatggcggg attttcgggc ggctacaagg gcatcttccc ggcggtcgcg
     5821 gacattgact cgatcatgca ctaccaccgc gcagcggtta tcggccacga gcgcagcacg
     5881 tggggcgtga tggaggacaa cccaacgcag gagcagatcc gggcaaacgg ctccctgctg
     5941 ccggtggact tctgcataaa cgtcacgctc aaccgcgccc gcgagatcac cggcttctac
     6001 tgcggcgacg ccctgaccgc ccacctggag ggctgcgagg ccagcaaagc ctccgcgatg
     6061 gtcgcctgcg agcgcgagta ccccatcgtc gtcaccacca acggcggcta ccccctggat
     6121 cagaacctgt accaggccgt aaaaggcatg tccgccgcct accagatcgt gtcagacggc
     6181 ggcctcatca tctccgtcgc ccgctgcaac gacggcttcc ccgagcacgg caacttccgc
     6241 aagatgctcc ttgaacacga ctcacccgag gcgatgcttg aaaccatcta cacccccggc
     6301 ttctcgctct atgaccagtg gcaggtgcag ctcttcgccc agatcctgaa aaaagcccgc
     6361 gtcggcctcc acagcgagat agaccccgag gccgtccgca gcgtccacct ggagccggtc
     6421 gcggacatca cggcccgcat ctccgaagaa ctagagagta tcggaagcga tgcccccata
     6481 gccgtcctcc ccgaaggacc catgaccgtt ccatatctgg agcatatgcc ggtaggagct
     6541 tgagacaaca atctcaagcg tagattgcca tgggcatgat ttgaatagcc ggagctttta
     6601 ttgatgaagg actgtcagag agcttaaata taccgccttt tcgcatctct gagaacactg
     6661 catccaggct cccgaactct aggtttctaa atgctgtacg ctggtacaag cttatctcat
     6721 cagccccctc gactagccgc gtcactttac cgagaaccga aaaatcgccg ctcaacaagt
     6781 tatcgaaggc gccttccggc agaaactcca gcgccaaggc aatgacaacg gtcaactcgt
     6841 cggcagttga cggtctcatc actgcatcta ggatcttgga ctgcgctaaa tcttccctaa
     6901 tacgttgcat tatttcgagc cccatcattg cttcgcggtc gagtgcgaat gggttgttag
     6961 aagatgcgtt ttgctgtccc tgaccccggt tcttcttccc tccaccggtt tgcgttcgca
     7021 gtgtctggtt agtaggcgtc tcaacgccga gcattgccat tgctcggaag acagcttcaa
     7081 gtgattcgtt aagcggactt ctagaaattt ggccgcgtat ctccaccaga taatcttgct
     7141 ctgcatcctt tagctgctgt atatcgtcaa tattattgat ctgaagaatt ctattgtcgt
     7201 tgattagagt atggcgcaga cgcatgaaga gtgacgcttc cgtgtgccgt cggaccagct
     7261 tgatttcttc gtcacttcct gagacctgct cacctgtgat cttccctctc aaatcaatat
     7321 ttaggagagt cgaaaagatg cccatgccag gaacgcccac gcgcgcctca ctcccggcac
     7381 ctagttttct ctgttgatct tgcctttgag ttacgtcgga gccataggct atgccgtcct
     7441 caagagcagc gacgaagctc gtcatcatgg gaacatcgag gtaaagtgga taaacaagat
     7501 ctattcgctc tgccacattt cctccgtctg tctaggcttc cttactcaac agattacagt
     7561 agctcaactt gtccgaatgc ttgaccactc ctaatcacga aacgttctgc ttgtgaaaca
     7621 accattcttt ggacgcttat tctactcagc tgaagagtga gccgcagatg tacaccttcg
     7681 tggataatga tgatgcctcg aagcgtggga gggtcgaaaa ccattagcgt ttgacgatta
     7741 catatttacg ttgtgggtgt tcactcgaat cgtttaatcg atactccgca ggatgctgct
     7801 tcttagtatg gtaagatcat ttgataaagt aaggagaatt aatatatcaa gggcaatatt
     7861 aacaggtaag ggccaactca cagttcccaa ggatgtgcgg gagcggctgg gtttgaagcg
     7921 tggggaccgg gtggtgttcg agttcgaggg ggattcggta cgtctgaagg tcgaggaacg
     7981 caagacgctc aaagagttga agggtagcct gccggccacg cgggagtatc cgggcaagga
     8041 cgccgagcgg caggcggcgc gtgaacacgt gattcgggag aagctcggcg ggtcttgagc
     8101 caagaggttt cttcctggtg gctggacgcg aacgtgctgt tgcgcttctt gaccggcgat
     8161 ccaccggagc ttgccgagcg tgcggccggg cttctggaga gagcccaacg gggagaggtc
     8221 ttgctgcggg tgcatgccgt ggtggtagcg gagacggttt gggtgctgga gtctttctac
     8281 gaatattcaa aggaggacac ctccggcgct ctcatccctc tactggaaca acccgcgcta
     8341 cgggtagagg gagcgagaac cgtcgtccga gcgctggaag cgatgtcgca gaacaacgtg
     8401 gacttcgccg acgcgctgct cgctgagacg gcccgctctc gtggcgaagg tgtcgcctcg
     8461 ttcgataggg acttccgcaa gcttggtatc gagtggcagg agccggacta gcgtgcgcga
     8521 tgaagcgcga acggcgcgcg aatcgtccgg tacttcagcc tgccagtagc cgtgtgagcc
     8581 gcaggtgcac gccctcgtcc tcgtcggtga cgtagtaggc gcggccttcg tgggtgacgg
     8641 cgatgccctc gacgcgcggg agggccggga actcgcggac gaactcggct tctagggtgc
     8701 cggagtgggc gtcgggcggt agggcgcagc agaagtgggt ggcgacggtc tcccggccgc
     8761 cggggtagtc ctgcacgagg acgctctgtt tgtcgcggct gtccacgttg ccggttacga
     8821 ggtgtagttg ctcgtcttct ccagtctgga gcagcgcaag gtcgcggact ccggctatct
     8881 ctccgttgtt gccgacagcg tccatggtcc agaagccacg gacctcgggt agctcgtcgc
     8941 cgggctcgaa gaggcggtcg atgccttcca gctcgatcag gatgggacgg ccgtcggcgg
     9001 cggtcgggaa gcggaggccg aggaggaggg tgccgctctc gcggaaggcg gcgccctcta
     9061 tgttgatcgg gtagtcgtcc tcgcgcacga gaccggacca gcgcttgttc tcttgctcgc
     9121 cgcgttggat ggtcgccgcg atgagggctt cgtgggactc ggggccgagc gggatgaggt
     9181 cgggaccgct ggatctcagg gcgtcgttta tgagacggtg gagggcgaag ctccggcggg
     9241 agattactat ctccattgcc gggtcttcgg tgacgtgtcc gactcccgcc tcgcggaagc
     9301 gggcgacgaa gccgcgtttg ggctgcagcg gaccggcctt gccgccgaag tgagagccga
     9361 ggatgtagac gaagcctgtg ctctcgtcgc gggtgatggc ctcggcgtcc tcggtcttgc
     9421 cggcgtcccc ggagaccctg gcgcgcaggc gctctatctc ccagccgtcg tcctccggag
     9481 cgtcgctgcg atggccgatg gcggctatgc agtcctcgac ctttccctcg tccagcaccg
     9541 cccagaaccc gatgtcccag ccgtgccgct tcagcagagc ctcgtcgccg atgctcagga
     9601 gccccgatgc ctcgttcggg tgcaggttga ggtccatctg gaagagtcca tcatccgcca
     9661 cgcgctgcct ccttctcttg acacgcactt tactacgatg cgcgccgcgc gaaggttcca
     9721 ggcggcttac gctaccggct ctggtctctc caggttacgg ttattcgtcc cagacctgga
     9781 cgccgacctg gcggaggatc ttgccgtccc gcaactcgca ggtctcaagg gcgaggacct
     9841 tcgtgccgtc cgggtactcg cacctctcgg caaaggctat gcgatcctcc ccaacgacct
     9901 cgttctccac acgatgcgtc atctcacgac cgaagatgtc cttcagatac tcggttatcg
     9961 cagccttgcc gcgcagctcg tgcggagcgc tcggcggggc gttgcggtct acggtgagta
    10021 tctctgcatc gtcgtcgtag aagctcaaca tccgttccag gtcgcgctct tctatcgcgc
    10081 gccgcagttc gccgatgtcg agtccgcccg aagctcgcgt taccaaaacg tactccttgc
    10141 tcccgttctc ccatgtcttg ccactatgtc tacaaccgcg agtttatacg tctacggagg
    10201 tggagggaac acagtaggga cgaattgcct atgcggattc cggcaatgac gggttcgcgg
    10261 cttcgttact cgtatctttg agcgatacct cttgcacgta tgtggaaccc tgtcaacgtc
    10321 cggaggtccg tggcccagcg gtagccgggt atagaatcag gctgcggaaa tcggtcggag
    10381 aggagtaacg atggcactga cggttggttc cggtccattc agcgagcggc acgaggggac
    10441 gttcaacttc gaggtagacg cgccgaaggg ccacgtcctg ttcctggaag actccccgcg
    10501 gcgagtgcgg gtgatgttcg gcggcgaaac agtcgcggac agccgccggg tgaagctcct
    10561 gcacgagacc gggcacttgc ctgtctacta cttcccggag gaggacgtcc gaatggacct
    10621 gctggaggcc acggaccact ccacgcactg cccattcaag ggcgacgctt cgtactggtc
    10681 cgtgaaggcg ggcggcaagg tggcggagaa cgccgtgtgg gggtatccgg agacgctgga
    10741 ggggtgcccc ccgatctccg gctacctcgc cttctactgg gacgcgatgg actcctggct
    10801 ggaggaggaa gaggaggtct tcgtgcaccc gcgcgaccct taccaccgcg tggacatcct
    10861 ggagagctcc cggcacgtca aggtgacggt gaacggcgag gtcgtcgccg agacggagcg
    10921 cccgaaggtc ctcttcgaga ccgccctgcc accccgctac tacatcccgc ccgacgacgt
    10981 gcggaccgac ctcctgaccc cgaccgagaa gcacaccggc tgcccgtaca agggcgtcgc
    11041 ctcatactgg tcggtcgagg ccggtggcga ggtggcggag gacctggtgt ggagttatcc
    11101 cgagcctatc gctgcggtcg agaagatccg ggaccacctc tgtttcttta acgagaaggt
    11161 ggacctggag gtggacggcg agccgcagcc gcgccccgag acgcagtggt cgtagggatt
    11221 agccccggcg ctcgccgagg ctaccagacc tgccagcgtg tcggcgttac ttggagcgcg
    11281 atggagtacg cccgcgtgaa gccttccaca tcgaagccga tgcgggctat gctctcccgg
    11341 tacttctcga tgtaagcgga gatctcgctg accggcggcg cgtcttcgac tatctcggcc
    11401 gcgccctcgg cgcgcaccac gtcgccgccg agggcgtttg agttgaggtt cagtccgacg
    11461 cgcgggttgc gggcgatgtt cctcagcttc cgccggcctt gctgggagta gatcaagaac
    11521 ctctccccgt cccacaggaa ccatacgggc accgactgcg gctgaccgtc ctcgcgcacg
    11581 gtagtgaacc agacgatctc ctcctccagc agccgacgct ccgctcgcct gccagcctcg
    11641 ctcgtggtgt cgagcatcct cgctcctttc cccaatctcc ggatatcgac aggtacacgc
    11701 atccaaccct ctgcggcgtc atcgtccctg tttcgctggg accggaccat tctactgccc
    11761 ggccgcccct acgtcgcccg gaggccgccc gcttcccgcc gctcccagtc ggctctcgta
    11821 accgcgtact cgacttcccc ccgctccgct ccctcgatgg gctccggcca ttctccgtga
    11881 aacgtccgcg ccagcgtcag gcccgatttc tccatcaccc gccgggaggc ggcgttgacg
    11941 gccatcgtct ccgcgaccac gcgccgcaca ccgagctcgg tgaagccctt gcgaattagg
    12001 gcgcgggatc cctcggtggc gtaacccttg ccccaggcgc tcctgcgtaa ccggtagccg
    12061 agctcgactt cgttagcggt tcttccctcc ggcggccgga agtggaacca tcccaggaac
    12121 tcccccgtgg acttctcgat ggccgcccag aagccgaacc ccttgaagcg ctcgtagtag
    12181 cggaggaacc ggggcagggt ctcgtcctcg atcacgccgc gggacgttgg tctgccgccg
    12241 ttgatgaaac gcatgacgtc aggatcccca tcgaggtcaa ccaggttgtc cacgtcagcc
    12301 ccggtgaacc gccgcagcaa catccgctcg gtctccagaa agatccacat cgcccgctcc
    12361 ttcccatgac gaacacgtcc agcatagcca ggtatgctac cggcggcaat cgcagcgagc
    12421 ccaagcacac catctcaccg ctccccaccg cgccggcccc gatcttggct cggcccgaaa
    12481 cctctatcct cacgttacgc tgatgttgca attttgttac gacgtgttac gttctggggc
    12541 tcatgcgtat gttgcagaca aaaagcattc tatccccggt ggttctgggg ctcatgttgt
    12601 tgggggcgat cttcctcctg tggaacgccg atcacgagcg cgttcgggag gtctccggta
    12661 tccggcactc cgcagtaacc ggggcgccgc aggccccgag tgggaacggc ggcgagggca
    12721 agatgatgga cgcttcctac tacggacacg agctggaggg cagcccgacg gccagcggcc
    12781 agcccttcga cccggagaag tacacggccg cctccaagac cctgccgcta gggacgaagc
    12841 tcaaggtaag ccacggcggc gagtctgtca gagtaaccgt caacgatcgc ggcccgtatg
    12901 tgcccggccg cgaactggac ctctccctgg cggcggccag aaagatagga ctcaccggcc
    12961 aggccgaagc gccggtccgc gtggtggtgc tttaaccctg tagctacccg gttcgtctat
    13021 ccgggagcca gccccttcct ccgcaactcc tccagggcct tctccgactt ctccaggtgt
    13081 cccatgccca tgccggagag gac
//
LOCUS       DASSMB010000157         3492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_922445, whole genome shotgun sequence.
ACCESSION   DASSMB010000157 DASSMB010000000
VERSION     DASSMB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922445"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFJ72_09330"
     CDS             complement(<1..1166)
                     /locus_tag="VFJ72_09330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7479701.1"
                     /translation="MRERAEVVIIGAGIVGCGVADHLTSMGRTDVVVLDGGPLYEAGG
                     STSHAPGGVFQTNPSKTMTEFAKYSVKRYSELMFDGEPCFLPVGGLEVAMTPERWKDL
                     RRKHGLATSWGLESEIIGPEECVEKVPLIDPTTIYGGFFVPTDGIARAVRACEVMAKA
                     STERGAEFHGETEVTGVEVEGGRVRAVETSRGRIETETIISCAGMWGPKIGRMVGMRV
                     PLTPMQHQLVWTTPLAELEGETREASHTLLRHQDRAMYFRHRRDHYGVGSYQHRAMPI
                     SAEDIPRWGETEVMPSIMEFTPQDFEKPWRDAVELMPALGKAEIAEPLNGLFSFTPDG
                     MPLMGESREARGFWIAEAVWITHAMGVARAMAEWIVSGVPSIDLATCDVHRFEP"
     gene            complement(1201..2520)
                     /locus_tag="VFJ72_09335"
     CDS             complement(1201..2520)
                     /locus_tag="VFJ72_09335"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7479702.1"
                     /translation="MTIDRADPYTRPRPNAAEDYTREETYEGTRAPVDFAVTLLPEAY
                     TDPEFHAIERERIFAASWIVVGCVSEVERSGQVIVAEVAGRSIIVTRDKAGRLQAFHN
                     VCRHRAARLLDYDCREVRNNRIRCPYHSWTYDLEGNCLGTPLFEGSDIPEDQRAVFDM
                     GGVKGFDKADYGLMPVSVDTWGFLVFVNLDPDAAPLAEQLGDLPGRLGGYGLEEWRIV
                     QERRYEVRADYKLVGENFMEYYHLPWVHPELVQVSRVEDHYRSQGPGMYMGFRTTPIL
                     QNTGDGGFLGLPPLSTLGGEDAVSGRFIWLFPNVAVNVLPSHAFIIIANPDGPRRTVE
                     RTVILAHPECTGAPGAEGEIEQLMHFWDLVNLQDIEIVERVQEGISNPAYRGGRMCYR
                     FEEPLHRFQNMVIDKMVGIDRVPPGDDEDETPMFGDSAESPGGGRGK"
BASE COUNT          616 a         1177 c         1035 g          664 t
ORIGIN      
        1 tggggctcga agcggtggac gtcgcaggtg gcgaggtcta tgctcgggac gccggagacg
       61 atccattcgg ccatcgcgcg ggcgacgccc atggcgtgcg tgatccagac ggcctccgcg
      121 atccagaacc ccctcgcctc ccgcgactcg cccatcagcg gcatcccgtc cggcgtgaat
      181 gagaagagac cgttcagcgg ctcggcgatt tcggccttgc cgagagcggg catcagctcc
      241 accgcgtccc gccagggctt ctcgaagtcc tgcggagtga actccatgat cgagggcatg
      301 acttctgtct ccccccagcg cgggatgtct tcggcggaga tgggcatggc ccggtgctgg
      361 taggagccga cgccgtaatg gtccctgcgg tgccggaagt acatcgcgcg gtcctggtgg
      421 cgcaggagcg tgtgagacgc ctccctggtc tctccctcaa gctccgcgag cggtgtggtc
      481 cagacgagtt ggtgctgcat cggcgtgagc gggacccgca tgccgaccat gcgtccgatc
      541 ttgggccccc acatccccgc gcagctaatt atcgtctccg tctcgatgcg gccccgtgag
      601 gtctcgaccg cccgcacccg gccaccctcc acctcgacgc cggttacctc ggtctcgccg
      661 tggaactccg cgccgcgctc cgttgacgcc ttcgccatca cctcgcaggc ccgaaccgcg
      721 cgcgcgatac cgtccgtggg cacgaagaag ccgccgtaga tcgtcgtcgg gtcgatgagc
      781 ggcaccttct caacacactc ttcggggccg atgatctccg actcaagacc ccaggaagtc
      841 gccagcccgt gcttcctgcg gaggtccttc caccgctcgg gcgtcatcgc gacctccagg
      901 ccgccgaccg gcaggaaaca gggctcaccg tcgaacatca gctccgagta gcgcttcacg
      961 gagtatttgg cgaactccgt catcgtcttc gacgggttgg tctggaagac tccgcccgga
     1021 gcgtgcgacg tggatccgcc cgcctcgtac agcggtccgc catcgaggac gaccacgtcc
     1081 gtccggccca tgctcgtcag gtgatccgcg acgccgcaac cgacgatgcc ggcgccgatg
     1141 atcaccactt ctgctctttc tctcaccgcg gtgcccagtc ctttccctcg ccggaagccg
     1201 tcactttccc ctgcccccgc cgggactctc cgcgctgtcc ccgaacatcg gcgtctcatc
     1261 ctcgtcgtcg ccgggcggca cccggtcgat cccgaccatc ttgtctatga ccatgttctg
     1321 gaagcggtgt agcggctcct cgaaacggta acacatgcgc ccgccccggt aagcggggtt
     1381 cgagatgccc tcctgcaccc gctcgacgat ctcgatgtcc tgcaagttga ccaggtccca
     1441 gaaatgcatc agttgctcga tctcaccctc cgcccccggc gctccggtgc attcgggatg
     1501 agcgaggatc accgtccgct ctaccgtccg ccgcggcccg tcggggttgg cgattatgat
     1561 gaaggcgtgg ctcggcaaaa cgttcacggc gacgttcggg aacaaccata tgaaacgtcc
     1621 gctcacggcg tcctccccgc caagggtact cagcggcggc aatcccagga aacctccgtc
     1681 ccctgtgttc tggaggatcg gggtggtccg aaaccccatg tacatccccg gcccctgaga
     1741 gcggtagtgg tcctcaaccc tcgatacctg aacaagctcc gggtggaccc acggcagatg
     1801 gtaatactcc atgaagttct cgccgacgag cttgtaatcc gcccgcacct cgtagcgcct
     1861 ctcctggact atccgccact cctccagtcc atatccgcca aggcgcccgg gcaggtcgcc
     1921 tagctgttcc gcgaggggcg cggcgtcggg gtcgaggttg acgaagacca gaaatcccca
     1981 ggtgtccacg ctcacgggca tgaggccgta atcggccttg tcgaaacctt taacgccgcc
     2041 catgtcgaag acggcgcgct gatcctccgg gatgtccgag ccctcgaaga gcggggttcc
     2101 gaggcagttg ccttcgaggt cgtaggtcca gctatggtag ggacaccgta tcctgttgtt
     2161 ccgcacctcg cggcagtcgt agtcgaggag cctggcggcc cggtgacggc agacgttgtg
     2221 aaacgcctgt agccgtccgg ccttgtcgcg cgtcacgatg atcgaacgtc cggccacctc
     2281 ggcaactatt acctgtccgc ttcgctcgac ctccgagacg cagccaacca ctatccagct
     2341 cgcggcgaag atccgctccc gctcgatggc gtggaactcc gggtcggtgt acgcctccgg
     2401 gagcagcgta accgcgaagt ccaccggcgc gcgcgtaccc tcgtaggtct cttctctggt
     2461 gtagtcttcg gcggcgttcg gacgcggtcg cgtgtacggg tccgcgcgat ctatggtcat
     2521 gtttctctcc tccttacggc cgtccagcga aacctcatcg tcaagagatc acgccctctt
     2581 cgtatgcgca gagcgagtgc ctcaacccgt cccgccagct ccagactcat tcctgcccac
     2641 catgacggaa cctcgtgatc taacagcaag ggccgggaaa cgaaacccca aaatgaaggc
     2701 ccaatccctt ttggcgacct caggttccac cagcgcgtat cggccgaacc ctcgctcccc
     2761 ttgcctctcg ccgcaccatt tggtccgtat agtggactct attcccatat cggaattggt
     2821 aacacagatg cctatgcagc gcaagaccag ttctcgacga tggccgggga cggctatcgc
     2881 tggaccatca gcggcacgag ctgcaaagcg tttgcgagat cacgggcagg aaaatgtctg
     2941 catctttctg gaggacgccg agaggctacc gacgagttat ggctacgtgg ttgggagccg
     3001 ggtaagacgt gttgttacac ggcgagcccg cggctccggt acgccgcggc ctcttgcatt
     3061 gctggcattg caggtctcgc gctacagaga gtcaggtgtt ctgtcttccg ggcagtaaga
     3121 gagcccggac aagcctggac atgtacccga tcatacacag gcgccgctgc aagccttgca
     3181 gaaggtttga cgctcacgga ctgctcggga atgtgccgat cgccttcgcg taaaatacgc
     3241 ctgacgcatg attcttagaa actgttttgc agagttaagc gttgatgaga gatcgtgcac
     3301 tcgcgtgccg cagtctcacc ggtatcattg gtggtcatag gtacggtccc ccgtcgcagg
     3361 agaggttctc ggttgaaaga tcccaaagca cgcttctcgg atagggccga gaactacgcc
     3421 aagtaccgac cggggtatcc gagagaggtc ctgcacttcc tagaagagag gggcactcta
     3481 agcaggaatt cg
//
LOCUS       DASSMB010000158         4787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_669760, whole genome shotgun sequence.
ACCESSION   DASSMB010000158 DASSMB010000000
VERSION     DASSMB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669760"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VFJ72_09340"
     CDS             <1..227
                     /locus_tag="VFJ72_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7479703.1"
                     /translation="TVVALSMGTIFGGAVLTETVFAWPGLGFLLADSLFKGDYNVARA
                     LLMISAALIVFFNLVADIAYSLVDPRVSYD"
     gene            240..1175
                     /locus_tag="VFJ72_09345"
     CDS             240..1175
                     /locus_tag="VFJ72_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479704.1"
                     /translation="MVDQHRDTGFGAQPSPFEVAEVGGEHYGKVRGRTQREIIWRRFR
                     RHRLALVGGAVLLLLYLAALATPVIAPYDFAKIDYTAFNQGPSLKHPMGTDRLGRDEL
                     TRVLYGGRISLMLGLAVGVFSTMIGATFGIVAGYYSKVVDTVTMGFVDFMLTLPYIPL
                     LLVMGSIFQFTAVTITIAIVILLWPRMARLVRGQVLVVRDQEYVQAAKAVGVSDVKIM
                     LRHILPNVVGVMVVEATLIVALAILFESAISFLGLGIQPPTPSWGNLLTDARSQMIEQ
                     WWLTWFPGLMIVITALCVNFLGDGLRDALDPKAVE"
     gene            1184..2197
                     /locus_tag="VFJ72_09350"
     CDS             1184..2197
                     /locus_tag="VFJ72_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479705.1"
                     /translation="MLEVNNLKTHFKTQDGVVKSVDGVSFSLEPGETLGIVGESGSGK
                     SVTALSIMQLNPKPPVEYPEGEILFEGQDLLKTSDARMQKIRGNDIAMIFQDPMTSLN
                     PVFTVGNQIREAIRIHQQVSKAEAQQRTVQVLKDVGIANPEQRAREYPHQFSGGMRQR
                     AMIAMGLACNPKVLIADEPTTALDVTIQAQILELMVDLQEKYGTAIIMITHDLGVVAQ
                     LADKVMVMYAGRAVEQGTTEEVFYDPLMPYSWSLLRSLPRLDTAGEVRLLPIKGSPPS
                     LIFLPQGCNFSPRCPFVKEECREIDPALEEKKPGHRAACILSVEELEQSKHRVDEQLG
                     VEG"
     gene            2194..3336
                     /locus_tag="VFJ72_09355"
     CDS             2194..3336
                     /locus_tag="VFJ72_09355"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET7479706.1"
                     /translation="MSERPNASPNGSQDFSGEHLLEAKNLKMHFPIKAGVLKRTVGHV
                     KAVDGVDLQVRKGETLGLVGESGCGKSTLARLILRLLEPTDGEVLFEGRDILKLGRSE
                     MLGVRRNMQIIFQDPYASLNPRMTVGNIIAEPLKTHKIEGNNKKRVQELLEIVGLSPE
                     HYNRYPHEFSGGQRQRIGVARAIALNPKLIICDEPVSALDVSIQAQVVNLLEDLQKEF
                     DLTYVFIAHDLSVVKHISDRVAVMYLGKIVEIADRKTLYNQPRHPYTNSLLSAIPIPD
                     PEKERERRRIVLTGDVPSPANPPSGCHFHTRCPRAQEYCKEHEPELELQENGVANGKN
                     ESGKVEAGNGSPSGTSEDHRAACFFPVMEGQRIEEPATSTPYRAGE"
     gene            3385..>4787
                     /locus_tag="VFJ72_09360"
     CDS             3385..>4787
                     /locus_tag="VFJ72_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7479707.1"
                     /translation="MSLRFRAFLILVLVAAVVGCGGPKTVSEPPHVAAPGEEEPISKV
                     GQGGTAHIALPASLAPACMNPYLPKCAGAGTFTGMTLESPLILGPSSEYRPLLAEAIP
                     SFEDGTLTLEPMTVEVRLRRGVNFSDGEPLTSADAKWTYEAAMRLARDDEISAPYSGF
                     ARVERVETPDARTVRIVFREPYSQWRDLLTAPILPRHIYGDRALKDLTLTRAPVGSGP
                     FLLKHFSPDGLKLIDTPRYWVSEPPYPNLSGLRVDFLDPGRVSDALSSGLADFGFFQD
                     SDAVPRSGNLLRATAPSRVALLVFNSRRLEDRTLRGRISESLDRESLPDGVVGGARAA
                     QSFVPPAVPGYEPAWQGGKAKDAGSESVKTGNTLDLVYPAEDPVYEELARKLSSQLSG
                     AGVGVASRPVSDGEYQDVLRRGDFDLALYTTSGTADLEPLLPSFPPDSRKVFEESLQD
                     VEGGGLVRAQEKFSEDAA"
BASE COUNT          881 a         1485 c         1559 g          862 t
ORIGIN      
        1 tgaccgtcgt ggctctcagc atgggcacta tcttcggagg ggcggtcctc accgagacag
       61 tcttcgcttg gccgggactc gggttcctgc tcgcggactc gctctttaag ggtgattaca
      121 acgtggcccg cgcgctgctc atgatcagcg ccgcgctcat agtgttcttc aatctagttg
      181 cggacatcgc gtactctctc gtggacccga gggtgagtta tgactagaga gttggtgaga
      241 tggtagacca gcacagagac acgggctttg gggctcaacc cagtccgttc gaggttgccg
      301 aggttggcgg ggagcattac ggtaaggtaa ggggccgcac tcaacgggag atcatctggc
      361 gccggttcag gcggcaccgg ctggcgctgg tcggaggagc ggtcctgctc ctgctctacc
      421 tggccgcgct tgcaacccct gtcatagcgc cctacgactt cgcgaagatt gactacacgg
      481 ccttcaacca gggaccgagc ctcaagcacc cgatgggtac cgaccgtctc ggacgcgacg
      541 agttgacccg cgtgttgtac ggaggacgga tctctctcat gttgggtctc gccgtgggtg
      601 tcttctcgac gatgatcggc gcgaccttcg gcatcgtcgc cgggtactac agcaaggtcg
      661 tggacacagt gacgatgggc ttcgtggact tcatgctcac ccttccctac atcccgctgt
      721 tgttggtaat ggggagcatc tttcagttca cggcggtcac cataaccatc gccattgtga
      781 tactgctctg gccgaggatg gcgcggctgg tgcgcgggca ggtgctcgtg gtcagggacc
      841 aggagtatgt gcaggcggcg aaggccgtcg gagtctcgga cgtgaagatc atgcttcggc
      901 atatcctgcc gaacgtggtt ggcgtcatgg tcgtcgaggc aacgctaatc gtggcgctgg
      961 cgatcctgtt cgagagcgct atctccttcc tcggattggg gattcagcct ccgactccct
     1021 cgtgggggaa cctgctgacc gacgcccggt cgcagatgat cgagcagtgg tggctcacct
     1081 ggttccccgg cctgatgata gtaatcacgg ccctgtgcgt taacttcctg ggcgatggtc
     1141 tgcgggacgc cctcgacccg aaggcggtgg agtaaccgtg gcgatgctgg aagtcaacaa
     1201 cctcaagacg cacttcaaga cccaggacgg cgtcgtgaag agcgtggatg gcgtctcatt
     1261 ctccctggag ccgggggaga ccctggggat cgtgggcgag tccggttccg gcaagagcgt
     1321 tacggctctc agtatcatgc agctaaaccc gaagccgccg gtagagtacc cggagggtga
     1381 gatcctcttc gagggccagg acctcctcaa gacctcggac gccaggatgc agaagatccg
     1441 gggcaacgac atcgccatga tctttcagga cccgatgacg agcctgaacc cggtctttac
     1501 cgtcggcaac cagatccggg aggccatccg catccaccag caggtctcga aggccgaagc
     1561 ccagcagaga accgtgcagg tccttaaaga cgtcggcatt gccaacccgg agcaaagggc
     1621 tcgcgagtac ccgcaccagt tctccggcgg gatgcgccag cgggccatga tcgcgatggg
     1681 actggcctgc aacccgaagg tccttatcgc cgacgaaccg accacggcgc tcgacgtgac
     1741 gatccaggcc cagatcctgg agctgatggt ggatctgcag gagaagtacg ggacggcaat
     1801 tatcatgatc acgcacgatc tgggcgtcgt agcccagcta gccgacaagg tgatggtgat
     1861 gtatgccgga cgagccgtcg agcagggcac gaccgaggaa gttttctacg atcccctgat
     1921 gccctactcg tggtcgctct tgcgctcgct gccgcggctg gatacggccg gggaggtgag
     1981 gctcttgccc ataaagggtt cgccgccgtc cctgatcttc ttgccgcagg gctgcaattt
     2041 cagcccgcgt tgcccgttcg tcaaggagga gtgccgcgag atagatccgg ctctcgaaga
     2101 gaagaagccg ggccatcggg cggcctgtat cctctccgtc gaggagctgg agcagagcaa
     2161 gcatcgggtg gatgagcaac tgggggtcga gggatgagcg agcggccaaa cgccagcccg
     2221 aacggaagcc aggatttttc gggcgagcat ctgttggagg cgaagaacct caagatgcac
     2281 ttccccataa aggccggggt cctcaagcgc accgtcgggc acgtgaaggc cgtggacggg
     2341 gtcgatctcc aggtgcgtaa aggtgagacg ctgggactcg tcggcgagtc cgggtgcggt
     2401 aagagcacgc tggcgcgcct gatcctccgt ctcttggaac cgaccgacgg cgaggtgctc
     2461 ttcgagggcc gggacatctt gaagctgggg cgaagcgaga tgctgggagt gcgccgcaac
     2521 atgcagatca tcttccagga cccatacgcg agcctgaacc ctcgtatgac cgtcggcaac
     2581 atcatcgccg aacccctgaa gacgcacaaa atagagggca ataacaagaa gcgggtgcag
     2641 gagttactgg agatcgtggg tctcagtccc gagcactaca accgctaccc gcacgagttc
     2701 tccggcggtc agcgccagag gatcggggtg gcgcgtgcga tagccttgaa ccccaagctc
     2761 atcatctgcg acgagccggt cagcgctctg gacgtctcca tccaggcgca ggtcgtgaac
     2821 ctcctcgaag acttgcagaa ggagttcgat ctcacctacg tgttcatcgc ccacgacctc
     2881 tcggtggtca agcacatctc tgatagggtg gcggtgatgt acctgggcaa gatcgtggag
     2941 atcgccgacc ggaagactct ctacaatcaa ccgcgccatc cctacaccaa ctcgctcctc
     3001 tcggccatcc ccatcccgga ccccgagaag gagcgcgagc gtcggcgcat agtcctgacc
     3061 ggcgacgtgc cctcgcccgc caacccgccc agcggctgcc acttccatac ccgctgcccg
     3121 cgcgcgcagg agtactgcaa ggagcacgag ccggagttgg aactgcagga aaacggagtt
     3181 gcgaacggca agaacgaatc cggtaaggta gaagccggca atggttcccc gagcggcacg
     3241 tccgaggacc atcgcgcggc gtgcttcttt ccggtgatgg agggacagag gatagaggaa
     3301 ccggcaacga gcacgcccta ccgcgccggg gagtagttca gcctccgccg gctccctatg
     3361 ccggagagcg agtagactct cgccatgtct ttgcggtttc gagcattctt gatcctggtc
     3421 ctcgtggccg ccgtggtggg atgcggcggt ccgaagaccg tgagcgaacc gccgcatgta
     3481 gcggctccag gcgaggaaga gccgatcagc aaggtcgggc agggcggtac ggctcatatc
     3541 gccctgcccg cctccctcgc gcccgcctgc atgaaccctt acctcccgaa gtgtgcgggc
     3601 gcagggacgt tcaccgggat gacgctggaa tctccgctca tcctcggccc ctcttccgag
     3661 taccggccgc tcctggccga ggccatcccc tcgttcgagg acgggacgct aaccctggag
     3721 ccgatgaccg tggaggtgcg gctgcgccgg ggcgtcaact tctccgacgg ggagcctctc
     3781 accagcgcgg acgcgaagtg gacctatgag gcggcgatgc gtctggcgcg ggacgatgaa
     3841 atctccgcgc cctactccgg cttcgcgcgc gtcgagcggg tagagactcc cgacgcccgg
     3901 acggtgcgca tcgtcttccg cgagccgtac tcgcagtggc gggacctcct taccgccccg
     3961 atactgcctc gccatatcta tggggaccgc gccctgaaag acctcactct gacgcgggcc
     4021 ccggtaggca gcggaccgtt cctcctcaag catttctcgc cggacggtct caagctcata
     4081 gacacgcccc ggtactgggt ctccgagcct ccgtatccga acctctccgg tctccgggtg
     4141 gacttcctgg atccaggccg cgtatcggac gctctatcgt ccgggctggc ggacttcggg
     4201 ttctttcagg actcggatgc ggtcccgagg tccggaaacc tgctgcgggc cacggctccg
     4261 tccagggtgg cgctgctcgt ctttaactcc cgcaggctag aagaccggac gctcaggggg
     4321 aggatctccg agagtctcga ccgtgagagc ctgccggacg gcgtggtcgg cggcgcgaga
     4381 gccgcgcaga gcttcgtccc cccggctgtc cccggctatg agcccgcgtg gcaaggcgga
     4441 aaggcaaaag acgccggctc agagtctgtg aagaccggca acactctgga tctcgtctat
     4501 ccggcggagg accccgtgta cgaggagttg gcccggaagc tctcctcgca actctccggg
     4561 gccggcgtcg gggtggcttc gcgtccggtg tccgatggcg agtaccagga cgtgttgcgg
     4621 cggggagatt tcgatctcgc gctctacacg accagcggga cggcggacct cgaaccgctc
     4681 ctcccgtcgt tcccgccgga ctcccgcaag gtattcgagg agagcctgca agatgttgag
     4741 ggtggcgggc tcgttcgggc gcaggagaag ttctccgaag acgccgc
//
LOCUS       DASSMB010000159         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_156164, whole genome shotgun sequence.
ACCESSION   DASSMB010000159 DASSMB010000000
VERSION     DASSMB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156164"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VFJ72_09365"
     CDS             <1..292
                     /locus_tag="VFJ72_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET7479708.1"
                     /translation="ARRVLADTGGLVSFELAAESREAAFRFLNSLELCVKAPSLGDVY
                     TLAIHPATSSHRELSPSRRERLGVKENLVRLSVGIEHPEDLISDLEQALERL"
     gene            422..1405
                     /locus_tag="VFJ72_09370"
     CDS             422..1405
                     /locus_tag="VFJ72_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479709.1"
                     /translation="MLLVTNGDIAVDRIEQAGIGGELLPWRDVLHEGPIPSDLPLDKM
                     REVRARYIAEQGWDDFEAVIGDFTRRDATLDGFRDHEEVVLWFEHDLYDQLQLIQLLD
                     WFSRRDLGATRLSMICVAEYLGNSEPDRLRTLFEDRDEVSARALDLGHEAWRAFTSTD
                     PTRISTLLREGTSALPFLKSAMLRHLQQFPSTKNGLSRSETQALEAISEGRIVLHEAF
                     ASSQELEERIFLGDTVFAGYLEGLSRGNEPLVLLEDGGMIEAPGTTGSAADFWNRRAI
                     VTDTGRAVLQGQKDWIEINGIDRWLGGVHLSGSEAEWRWDEPTQQLQRTAA"
     gene            1489..2421
                     /locus_tag="VFJ72_09375"
     CDS             1489..2421
                     /locus_tag="VFJ72_09375"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET7479710.1"
                     /translation="MARTHYFPEDRVHYVWDNELEPAITVSPGDTVVYNTREVSDGQI
                     TPDSTADALATFDWDRVYPLSGPVGVEGAEPGDTLVVEVIDIHTVGWGWTAVIPGFGL
                     LPEDFPDAYLKIFDLSNGDFTLFNDEIAIPIEPFFGTMGVNPNDPAKPAIMPPGPFGG
                     NMDTRHITKGSVLYLPVQMSGALFSCGDAHAAQGDGEVCVTGIESPMYAALRFDLIKG
                     KTIPAPQFQTPGALTPWSEGAGWYATMGVNSDLMVAAQDSLRAMVEHISTTYGLEPVE
                     AYVLASLVVDLKISEIVDQPNWIVSSYLPLAIFS"
     gene            2450..>2828
                     /locus_tag="VFJ72_09380"
     CDS             2450..>2828
                     /locus_tag="VFJ72_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7479711.1"
                     /translation="MPDPEALAFDMYGTLVDPIGIQKELESFLPEGAPSVAASWRQKQ
                     LEYTFRLTAMERYEDFEQVTRRALDYALAASGRDLDAGQKDTLMQRYNDLERFPDVVP
                     GLERLKEAGHAMIVFSNGTPRMLE"
BASE COUNT          554 a          905 c          874 g          495 t
ORIGIN      
        1 cgcccgacgt gtgctcgccg ataccggagg cctggtctcc ttcgagctgg cagcggagag
       61 tcgtgaagcc gccttccgat tcctcaactc cctggagctg tgcgtcaagg cgccgagcct
      121 cggggacgtc tacaccctgg ctatccaccc ggcgacctcc tcgcaccgcg agctctcgcc
      181 gtcgcgccgg gagcggctcg gggtcaagga gaacctcgtg cggctctcgg tcggtatcga
      241 gcacccggaa gatttgatct ccgacctcga acaggcgctc gaacgacttt aggaatcagc
      301 ttcttcctgg gaagcgctgt agatcccgta tttcggtgat tgttattcgg catctctccg
      361 tacaatcccg gtagcgcgcc tcttgaaccg ctgccgtggt gatcagagat aggagagaag
      421 catgttgctc gtcaccaatg gggacatagc cgtggaccgg atcgaacagg ctggcatagg
      481 tggcgagctt ctcccctgga gagacgtctt gcacgaaggt cccatcccct cagatcttcc
      541 gctcgacaag atgcgggagg tgcgcgctcg ctacatcgcg gagcaagggt gggatgattt
      601 cgaggcggtg ataggcgatt tcacccgtcg agacgctacc ctggacgggt tccgcgacca
      661 cgaagaggtg gtcctctggt tcgagcacga cctctacgat cagctacaac tcatccaact
      721 gctcgactgg ttctcgcggc gagaccttgg cgccaccaga ctcagcatga tctgcgtcgc
      781 cgaatacctg gggaactcgg aacccgaccg cctgcggacc ctattcgagg atcgcgacga
      841 ggtgtccgcc cgggcgctgg atctcgggca cgaagcctgg agagccttta cttcgacgga
      901 ccccacacgg atctcgaccc tgcttcggga aggcacttcg gccttgcctt tcctaaagag
      961 cgctatgctc cgtcacttgc agcagttccc atccacgaag aacggcctct cccgctccga
     1021 gacgcaggcg cttgaggcga tctcggaagg aagaatcgta ctccacgaag ccttcgcctc
     1081 atctcaggaa ctcgaggaac gcatctttct cggtgacacg gtcttcgccg gatacctgga
     1141 aggtttgagc cggggaaacg agccgctcgt gctgctcgaa gacggcggca tgatagaggc
     1201 tccgggtacg acggggtctg ccgcggactt ctggaacaga cgagccatag tcaccgacac
     1261 cggacgcgcg gtcctccagg gccaaaaaga ctggatcgag atcaacggca tagatcgctg
     1321 gttgggggga gtacacctga gcggtagcga agccgaatgg cgctgggacg agcccaccca
     1381 acagctccaa cgcaccgccg cctgaacacg tcagagtaga aataatctcc gcaagctgcg
     1441 aaagtatcgt ctgggtaaga gaaacgagac cggaggaggc ttgtggctat ggcgcggacg
     1501 cactatttcc cggaggacag ggtccactac gtctgggaca acgagctgga gccggccatc
     1561 acggtctcgc cgggagacac ggtcgtctac aacacccgcg aggtctctga cggtcagatc
     1621 acaccggact cgaccgccga cgccctcgct accttcgact gggaccgcgt ctacccgctc
     1681 tccggccccg tgggcgtaga gggcgcagaa cccggcgaca cgctggtagt ggaggtaata
     1741 gacatccaca ccgtcgggtg gggctggacc gccgtgatcc ccggcttcgg acttctcccg
     1801 gaggacttcc cggacgccta cctgaagata ttcgacctct ccaacggcga ctttacgctc
     1861 tttaacgacg aaatcgccat ccccatagaa ccgttcttcg gcacgatggg cgtcaacccc
     1921 aacgacccgg cgaagcccgc cataatgccg cccggacctt tcggcggcaa catggacacc
     1981 cgtcacatca cgaagggctc ggtcctgtac ctgccggtgc agatgtccgg ggcgctcttc
     2041 tcctgcggcg acgcgcacgc ggcgcagggc gacggtgagg tctgcgtaac cggcatcgag
     2101 agcccaatgt acgcggcctt acgcttcgac ctgatcaagg gcaagaccat ccccgcgccg
     2161 cagttccaga cgcccggcgc gctgaccccg tggtccgagg gcgccggctg gtacgccacg
     2221 atgggtgtca actccgacct gatggtcgcg gcacaggact cgctgcgggc gatggtcgag
     2281 cacatcagca cgacgtatgg cctggagccg gtagaggcct acgtgctggc gagcctggtg
     2341 gtggatttga agatcagtga gatagtggac cagccgaact ggatcgtctc ctcctacctg
     2401 ccgctggcta tattcagcta gtccggctgg aaacccgaaa ggaggttcga tgccggaccc
     2461 ggaagccctc gcttttgata tgtacggcac gctggtggac cctatcggga tacagaagga
     2521 actggagagc ttcctcccgg aaggcgcgcc gtccgtggcc gcgtcgtggc ggcagaagca
     2581 gctcgaatac acgttccgcc tcacggcgat ggagcggtac gaggacttcg agcaggtgac
     2641 gcgccgcgcc ctggactacg cgctcgccgc ctccggtaga gatctggacg ccgggcagaa
     2701 ggacacgctc atgcagcgct acaacgacct ggagcgcttc cccgacgtcg taccggggct
     2761 ggagcgcctc aaggaggccg gtcacgccat gatcgtcttc tcaaacggca ccccccgcat
     2821 gctcgaag
//
LOCUS       DASSMB010000160         8799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1051416, whole genome shotgun sequence.
ACCESSION   DASSMB010000160 DASSMB010000000
VERSION     DASSMB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051416"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1455)
                     /gene="cimA"
                     /locus_tag="VFJ72_09385"
     CDS             complement(<1..1455)
                     /gene="cimA"
                     /locus_tag="VFJ72_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET7479712.1"
                     /translation="MSIKLFDTTLRDGTQREGVSLTAEDKARIARELDALGLHYIEAG
                     FPASNPKDLEFFEGFDASELANARLVAFTRARRPNGRAEDDPAVTLLPELSAPVACIV
                     AKTWKLHVEKVLRTTPEENIESIKDTVAYLAGAGKEVIFDAEHYFDGFRDDPEYSLAV
                     LRAAAEAGATTLVLCDTNGGTLPTELKAIVSRTVREFTEMDGVEVGIHTHNDSGCGVA
                     NALVAIEAGATHVQGTINGVGERTGNCDLVTTIANLQLKMGLNVMDGENLKKLTEVSN
                     YVSELMNLTPDSHRPYVGRSAFAHKGGLHVDGISKDPATYEHVAPEVVGNVRRTPVGE
                     LSGKNSIRAKAEELGIEAGDATEILSAIKQREYEGYHYEAADASLALLIGRTTGESKP
                     LFELESFRIISEKRAGGETTVEATIKLLVKGQRVISTAEGNGPVNALDQALRRAIEPH
                     YPELKEIHLSNYKVRILDEHRATAATTRVLIDSTD"
     gene            complement(1452..2519)
                     /gene="leuB"
                     /locus_tag="VFJ72_09390"
     CDS             complement(1452..2519)
                     /gene="leuB"
                     /locus_tag="VFJ72_09390"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET7479713.1"
                     /translation="MRDSFDILLLPGDGIGPEVVGAACEVIDVAAGHYGVKLNYEERK
                     IGGVAIRDEGAPVSDETLEAARASDSVLLGAVGHPDFDKGSVRPEAGLLKIRKHLGAF
                     ANLRPVEALPALLSASTLKKEVVEGVDILIIRELTGGAYFGEKEEGTERASDLSVYTR
                     EEVERVTRVAFEAAKKRKRRVTSVDKANVMATSRLWRGVVEEVAEDYPEVELDHVLVD
                     AAAMFLIREPRRFDVLLTENLFGDILSDEAAMLPGSMGMLPSASLGEPGTPGIFEPVH
                     GSAPDIAGQGKANPYATILSAAMMFRYTLGRPDIAEAIEQGVSVALEAHFLTPDLGGT
                     KTTEDVTKAVSRWVAAGEGVA"
     gene            complement(2530..3297)
                     /locus_tag="VFJ72_09395"
     CDS             complement(2530..3297)
                     /locus_tag="VFJ72_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479714.1"
                     /translation="MGRDGSAVEVPHRTSAVPAGYESLDGGVPVLHAPRLLERAREIQ
                     GYLKEGSGALAGILNVEPPDLASILVADEDWKDAPRENSRPYPPGLPYFTRSVNPPAL
                     ILPETLSRAFRPDTEATLPLTVWHELAHAFLLQREVVRTPAWLRELVPQAMAAAVARR
                     EGLLAGHLARIDHVPGFTVRSFEGPASAGDQMAFQNLLLALGAAALEEFGEGFLKRLV
                     YALWDEDEVVGGVRAEELLAGALGPRGREWLQSRVEF"
     gene            complement(3273..4814)
                     /locus_tag="VFJ72_09400"
     CDS             complement(3273..4814)
                     /locus_tag="VFJ72_09400"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET7479715.1"
                     /translation="MRRVEIFDTTLRDGEQSPGISLSVEEKVEIARQLARLEVDVIEA
                     GFPITSEGDFESVSRIAAEVKGPRIAGLARIHEQDIVRAWEAVQWSERPRIHTFVGTS
                     DLHIEHQMRSNQEDILRKAGEAVAFAKSLCENVEFSPMDATRTDIGYLSEVVAAAVDA
                     GADVINIADTVGYTTPVEFAAFLKELQERVPGLADRVLSVHCHDDLGMAVANSLAGVE
                     VGARQIEVAVNGIGERAGNASLEEVVMALATRKDFYGVEVGVDTRQLANTSRMVSNIT
                     GYEVPPNKAIVGRNAFLHESGIHQDGVLKDRRTFEIMTPKDIGLEGTNIFLGKHSGRH
                     ALKDALEELGYYVEGEVLKRAFERFKQVADHKKTVTAADLEAIAADEVGSFEGKFVME
                     SFRVVAATGRQSRAAVTISHADHGTFEAEAEGEGPVDAVFRAIDAATNIKGRLTDFRI
                     DAVTGGKDALGEVRVSVEFEGSEYAGRGLSQDVVEAAARAYVRAVNVYTAGRVGAKWD
                     ATVAP"
     gene            complement(5147..5788)
                     /locus_tag="VFJ72_09405"
     CDS             complement(5147..5788)
                     /locus_tag="VFJ72_09405"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HET7479716.1"
                     /translation="MVVVDTYRASTTIAVLVRKGARVIPVASIEEAARYPGADYRIGE
                     RGSAKVRGFDFGNSPTEVQASDIAPGSTVVLSSTNGTRIIEAARGAPAIIAGAFVNAR
                     AVADELANGEPDSRVAVIGCGWQGRRASEDESAAGAILARLRENGASLDERARAIVDA
                     YHRRPERALRTNSAARRLKRLDYHRDLDFCLAPNTVPVVPRLADKAFTSVDGA"
     gene            5992..6411
                     /locus_tag="VFJ72_09410"
     CDS             5992..6411
                     /locus_tag="VFJ72_09410"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7479717.1"
                     /translation="MAEPKTKETISLEDAKRIIEAAQKKATEINCPMDIAVTDAGGNL
                     KAHVRMDGAFVGSITISINKAYTAIAFQQETKNLQEVTRPDGPIFGLSDAHGGRLVVF
                     PGGIPLVRDGEIIGAIGVSTGTVEQDQEVAEAGAAAL"
     gene            complement(6488..7444)
                     /locus_tag="VFJ72_09415"
     CDS             complement(6488..7444)
                     /locus_tag="VFJ72_09415"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HET7479718.1"
                     /translation="MARTAFAANDAEWCYHGGEMVRMGDVRLSPATHALNYGTGVFEG
                     IRAYWSEGRGTLQVLKMREHYERFEKSCRLLRIDMPNTVDELCDITLEILKRNAPRED
                     TYIRPLAYKAAESVGVNLKGRSELSVFTVPMGNYVELTGLKCCVSSWRRVADNAIPTR
                     AKCTGSYVNTALAVDAAHRAGYDDAIFLTQSGFVSEASAANIFVVRKGQLITPPVTAD
                     ILEGITRDAVMELAEKELGMPAEQRDVGRTELYAADEVFLTGTGFQIAPVVEVDDRPI
                     GTGSIGPVAERLQEIYFKAARGELDEYTDWTVAVEVAQRTAG"
     gene            complement(7444..8466)
                     /gene="ilvC"
                     /locus_tag="VFJ72_09420"
     CDS             complement(7444..8466)
                     /gene="ilvC"
                     /locus_tag="VFJ72_09420"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7479719.1"
                     /translation="MARVYREADIGNEITQKKVAILGYGSQGHAHALNLKDSGCEVTV
                     GLYEGSKSWPKAEQEGLQVMTIADAVQWADVVMMLLPDEKQPAVFRAEVEPNLSEGML
                     MLFAHGFNIHFNQIVVPPEVDLGLVAPKGPGHVLRRLYVEGKGMPSLFAVQNDASGQA
                     RDVVLSYARGIGSGRAGIIETTFAEETETDLFGEQAVLCGGLSALLTAGFETLVEAGY
                     QPELAYYECVNELKLIVDLIYEGGLAQMRYSISNTAEYGDYTAGPKIVDEGTKQRMRQ
                     ILTDIQTGKWAKEWVLENQAGGSSFLAMRRREAESQVEKVGQELRALAAEGVEAPAGN
                     PAGGGS"
     gene            complement(8484..8762)
                     /locus_tag="VFJ72_09425"
     CDS             complement(8484..8762)
                     /locus_tag="VFJ72_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479720.1"
                     /translation="MAPSSAGSTTDVEPIEIEARIAGGILALNRFLMTLQNKRMPVAR
                     ISVDGDRDGTRIGIALDCPEETARRYATLLESLEDVEEIRFAERPAQK"
BASE COUNT         1361 a         3043 c         2781 g         1614 t
ORIGIN      
        1 gtcggtcgag tctatgagga cgcgggtggt ggcggcggtg gcgcggtgct cgtccaggat
       61 gcggaccttg tagttggaga ggtggatctc ttttagctcc gggtagtgcg gctcgatggc
      121 ccttctgagc gcctggtcca gcgcgttgac gggtccgttg ccctcggcgg tcgagatgac
      181 gcgctgtccc ttcaccaaca gctttatggt cgcctcgacc gtcgtctcgc cgccggcccg
      241 cttctcggaa atgatgcgga agctctccag ctcgaagagc ggcttcgatt cacccgtcgt
      301 acgcccgatg agcagcgcga gcgacgcatc ggcggcctcg taatggtagc cctcgtactc
      361 ccgctgcttt atggcggaga ggatctccgt cgcgtccccg gcctcaatcc ctagctcctc
      421 cgccttcgcc cggatggagt tcttccccga gagctccccg accggtgtcc gccgcacgtt
      481 cccgacgacc tccggcgcga cgtgctcgta ggtcgcaggg tctttggaga tgccgtccac
      541 gtgcagcccg cccttgtgcg cgaaggcgct ccggcccacg tagggacggt gagagtcggg
      601 cgtcaggttc atcagctcgg agacgtagtt ggagacctcg gtgagcttct tgaggttctc
      661 gccgtccatc acgttcaggc ccatctttag ctgcaggttg gcgatggtgg tcacgaggtc
      721 gcagtttccg gtgcgttccc cgacgccgtt tatggtgccc tgcacgtgcg tcgccccggc
      781 ctctatagcc accagcgcgt tcgccacccc gcacccggag tcgttgtgcg tgtgtatgcc
      841 gacctccacg ccgtccattt ccgtgaactc gcgcacggtg cggctgacga tcgccttcaa
      901 ctccgtcggc agcgtcccgc cgttggtgtc gcacagtacg agcgtcgtcg cgcccgcctc
      961 ggcggcggct cgcaggacgg ccagagaata ctccgggtcg tcccggaagc cgtcgaagta
     1021 gtgctcggcg tcgaagatga cctccttgcc ggcgcccgcc aggtacgcga ccgtgtcctt
     1081 tatggactcg atgttctcct ccggcgtcgt cctcaggacc ttctcgacgt gcagcttcca
     1141 cgtcttggct acgatgcagg ccacgggtgc ggagagctcc ggtagaagcg ttaccgcagg
     1201 gtcgtcctcg gccctgccgt tcggacgccg cgctcgcgta aaggcgacga gcctcgcgtt
     1261 cgcaagctcc gaggcgtcga agccctcgaa gaactccagg tccttcgggt tagaggccgg
     1321 gaaacccgct tctatgtagt gaagccctag agcgtccagc tcgcgggcta tgcgggcttt
     1381 gtcctcggcg gtcaggctca cgccctcgcg ctgggtgccg tctcttagag tggtgtcgaa
     1441 tagttttatg ctcatgccac gccctctccc gccgctaccc agcggctcac ggctttcgtc
     1501 acgtcttcgg tcgtcttggt cccgcccagg tcgggggtaa ggaaatgagc ttcgagcgcc
     1561 acggagacgc cttgctctat ggcctcggca atatccgggc ggcctagcgt gtagcggaac
     1621 atcatcgccg ctgacaggat ggtcgcgtag gggtttgcct tgccctggcc ggcgatgtcc
     1681 ggggcggagc cgtggacggg ttcgaagatg ccgggcgtgc cgggctctcc cagggaagcg
     1741 ctcgggagca tacccatcga gccggggagc atggccgcct cgtcggagag gatgtcgccg
     1801 aagaggttct cggtcagcag cacgtcgaag cggcgcggct cccggatcag aaacatcgcc
     1861 gccgcgtcta ccaggacgtg gtcaagttcg acctccggat aatcttccgc gacctcctcg
     1921 accacgcccc gccacagccg gctcgtcgcc atcacgttcg ccttgtccac gctcgtcacc
     1981 cgccgcttgc gctttttcgc ggcctcgaac gcgacccgcg tcacccgctc gacttcttcc
     2041 cgcgtgtaca cggaaaggtc gctagcccgc tccgtcccct cctctttctc tccgaagtac
     2101 gccccgccgg tcagctcccg gataatgagg atgtccactc cctcgacgac ctctttcttg
     2161 agcgtggagg ctgagaggag cgccggcagc gcctcgacgg gccgcaggtt tgcgaacgcc
     2221 ccgaggtgct tgcggatctt caggagcccg gcctcgggtc ggacggaacc tttatcgaag
     2281 tccgggtggc cgaccgcgcc gagcagcacc gagtccgagg cgcgggcggc ttcgagcgtc
     2341 tcgtcagaga cgggagcgcc ctcgtcgcgg atggctaccc cgcctatctt ccgctcctcg
     2401 tagttgagct ttacgccgta gtgtcccgcc gccacgtcta tcacctcgca agcggccccg
     2461 accacctccg ggccgatacc gtcgccaggc aatagcagaa tgtcgaacga atcgcgcatg
     2521 ataatctccc tagaattcca ctctcgattg caaccactcg cgcccgcgcg ggccgagcgc
     2581 cccggcgagc aactcctccg cccgcacccc accgacgacc tcgtcttcgt cccagagcgc
     2641 gtacacgagc ctcttcagga agccttctcc gaactcttcc agcgccgccg cgccgagcgc
     2701 gagcaagagg ttctggaatg ccatctggtc cccggcgctc gccggacctt caaagctccg
     2761 cacggtgaag cccggcacgt ggtctatccg ggcgaggtgt ccggcgagca gaccctcccg
     2821 gcgcgcgacc gccgccgcca ttgcctgcgg cacgagctcc cggagccatg ccggggtcct
     2881 cactacctcc cgctgaagca ggaaggcgtg cgccagctcg tgccagacgg tcagcggcag
     2941 cgtagcctcc gtatccggcc ggaacgcccg cgagagcgtc tccggcagga tcagcgccgg
     3001 agggttcacc gaacgggtga aatacggcag gccgggcggg tagggacggg agttctcgcg
     3061 cggcgcgtcc ttccaatcct cgtccgcaac cagtatggac gcgagatccg gcggctcgac
     3121 attcagtatc ccggcgagcg cgccagagcc ctctttcagg tagccctgta tctcccgcgc
     3181 ccgctccaat agacgcggag cgtggagcac cgggacgccg ccgtccaggc tctcgtagcc
     3241 cgccgggacg gcgctcgttc tatgcgggac ctctacggcg ctaccgtcgc gtcccacttc
     3301 gctccgaccc tgcccgccgt gtagacgttg accgcccgca cgtaggcccg cgccgccgcc
     3361 tcgaccacgt cctggctgag gccgcgcccg gcgtactcgc tgccctcgaa ctcgaccgag
     3421 acgcgcacct cgccgagggc gtccttgccg cccgtgacgg cgtcgatgcg gaagtccgtc
     3481 aggcggccct tgatgttcgt cgcggcgtct atggcgcgga agaccgcgtc caccggaccc
     3541 tcgccctcgg cctcggcctc gaacgtgccg tggtcggcgt gcgagatcgt gacggccgcc
     3601 cggctctggc ggccggttgc ggcgacgacc cggaaagact ccatcacgaa cttgccctcg
     3661 aaggagccaa cctcgtcggc ggcgatggct tccaggtcgg cggcggtgac ggtcttcttg
     3721 tggtccgcga cctgcttgaa gcgctcgaac gcccgcttga gcacctcgcc ctcgacgtag
     3781 tagccgagct cctccagggc gtccttcagg gcgtgacgtc ccgagtgctt gccaaggaag
     3841 atgttggtgc cttccaggcc gatgtccttc ggggtcatga tctcgaacgt ccgccggtcc
     3901 ttcaagacgc cgtcctggtg tatccccgac tcgtgcagga aggcgttgcg cccgacgatg
     3961 gctttgttcg gcggcacctc gtagccggtg atgttggata ccatccgcga ggtgttggcg
     4021 agctggcggg tgtccacacc gacctcgacg ccgtagaagt ccttgcgcgt ggcgagcgcc
     4081 atcacgacct cctccaggga ggcgttgccc gcccgctctc caatgccgtt gacggcgacc
     4141 tcgatctggc gcgccccgac ctcgacgccc gcgagcgagt tcgcgaccgc catccccagg
     4201 tcgtcgtggc agtgcaccga gagcacccgg tccgcgagac ccggcacccg ctcctggagc
     4261 tccttcagga acgccgcgaa ctccaccggc gtcgtgtacc cgacggtgtc ggctatgttg
     4321 atcacgtccg ccccggcatc gaccgccgcc gcgacgacct cggagaggta gccgatatcc
     4381 gtgcgggtgg cgtccatcgg ggagaactcg acgttctcgc acaggctctt ggcgaaggcg
     4441 acggcctcgc ccgctttacg caggatgtcc tcctggttcg agcgcatctg gtgctcgatg
     4501 tgcaggtccg aggtgccgac gaaggtgtgg atgcggggcc tctccgacca ctggaccgcc
     4561 tcccacgcgc gcacgatgtc ctgctcgtgg atgcgggcga gaccggcgat acgcggtccc
     4621 ttcacctcgg cggcgatgcg ggagaccgac tcgaagtctc cctcggaggt gatcgggaat
     4681 ccggcctcga tcacgtcgac ctccagacgc gcgagctggc gggctatctc taccttctct
     4741 tctaccgaca gggaaatccc cggcgactgc tcgccatccc gaagcgtggt gtcgaagatc
     4801 tctactcgac gcatatgtct ctcatctccc tacgttgaaa acttggattg aaactgcggc
     4861 tcttgagttt ttaggtggcc gctcggagcg tggctagccc ggctagagac cgggacgggt
     4921 aattcgcccg gcgaccaccc tggttagtcg taggatgacg aaagaaagtg gcacgttgtt
     4981 agagactcac cttcatgtga ggcgcaagta tctcatctgt agccgcttgc tgcaacctgg
     5041 acgccggcgg ctgtgtatcc gaagaggaaa cttccaactc ctccgcgcct gtctccgcta
     5101 gcggattttc acccatgaaa ccggcgaata gggtgagaag cataacttat gccccgtcca
     5161 cgctagtaaa agccttgtcc gcgaggcgcg gcacgaccgg gaccgtgttc ggggcgaggc
     5221 agaagtcgag gtccctgtgg taatccagac gcttgagccg ccgggcggcg ctgttcgtcc
     5281 gcagggctct ctccgggcgc cggtggtagg cgtccacgat ggctctcgct ctctcgtcca
     5341 ggctcgcgcc gttttcgcgc aggcgggcga ggatcgcgcc cgccgccgac tcgtcttcgg
     5401 aggcccgccg tccctgccag ccgcagccga tcacggctac ccgtgagtcg ggctccccgt
     5461 tcgccaactc gtcggcgacg gcgcgggcgt tgacgaaggc gcccgcaatg atggccggag
     5521 cgccacgggc ggcctcgatg atcctggtcc cgttcgtgct ggagaggacg accgtggaac
     5581 cgggcgcaat atccgacgct tggacctccg tgggcgagtt cccaaagtcg aagccgcgta
     5641 ccttcgcgct cccgcgctct ccgatgcggt agtccgctcc gggatagcgc gcggcctcct
     5701 cgatggaggc caccgggatc acgcgggcgc ccttgcgcac caggaccgcg atcgtggtgc
     5761 tcgcccggta tgtgtcgacc accaccactg ccgcgccctc gcgcgctgcc ctgcgagcgc
     5821 ctttttctcc cgcggcataa gtggcaagca cgttactcgg actctcccat cattcgcgcg
     5881 attatacgcg cgaatagcgt ttctggagaa cgataccttg cgcgggactc gcactttgat
     5941 agcatgcgcg gcggaaaggt gtagggcaat gtaagggaaa ggaaaaccag catggccgaa
     6001 ccgaagacca aagagaccat ctcgctggag gacgcgaaga ggatcatcga agcggcgcag
     6061 aagaaggcta cggagatcaa ctgcccgatg gacatagccg tcaccgacgc cggtggcaac
     6121 ctcaaggccc acgtccgcat ggacggagcg ttcgtcggga gcattacgat ctccataaac
     6181 aaggcctaca cggctatcgc tttccagcag gagaccaaga acctgcagga ggtgacgcgg
     6241 ccggacggcc cgatcttcgg cctctccgac gcgcacggcg gacggctggt ggtgttccct
     6301 ggcggcatcc cgctggtgag ggacggggag ataatcgggg cgataggggt ctccacgggg
     6361 accgtcgagc aggaccagga ggtcgccgag gccggcgccg ccgcgctcta gcgaggctcg
     6421 caatagttca caagcgcggc gcacggtacc tcgccgtgcg ccgcgctcta tctacaaaaa
     6481 atagcgtcta tccggcggta cgttgcgcga cctcgacggc gacggtccag tcggtgtact
     6541 cgtccaactc gccgcgcgcg gccttgaagt agatctcctg cagcctctcg gcgaccgggc
     6601 cgatgctgcc ggtccctatc ggacggtcgt ccacctcgac gacgggcgct atctggaagc
     6661 cggtgccggt gaggaagacc tcgtcggcgg cgtagagctc ggtgcggccc acgtcgcgtt
     6721 gctcggccgg catcccgagt tctttctccg caagctccat caccgcgtcg cgggtgatgc
     6781 cctccaggat gtccgccgtc acgggcggcg tgatgagttg ccccttcctg accacgaaga
     6841 tgtttgccgc gctcgcctcg gagacgaagc cggactgggt gaggaagata gcgtcgtcgt
     6901 agcccgcccg gtgcgccgcg tccaccgcga gcgccgtgtt gacgtacgag ccggtgcatt
     6961 tggcgcgggt cgggatcgcg ttgtccgcca cccgccgcca ggaggagacg cagcacttga
     7021 gacccgtgag ttccacgtag ttgcccatcg ggaccgtgaa gaccgagagc tcggaacgac
     7081 ctttcaggtt caccccgacc gactcggcgg ccttgtacgc tagcggccgg atgtaggtgt
     7141 cctccctcgg agcgttgcgc ttgaggatct caagcgttat gtcgcacagc tcatccacgg
     7201 tgttcggcat gtctatccgc agcaggcggc aggacttctc gaaccgctcg taatgctcgc
     7261 gcatcttgag gacctgtaag gttccccggc cctcgctcca gtacgcccgg atgccctcga
     7321 agacgcccgt gccgtagttt agggcgtggg tggcggggga gagccgcacg tcgcccatcc
     7381 tgaccatctc accgccgtgg tagcaccact ccgcgtcgtt tgcggcaaat gccgtgcgcg
     7441 ccatcaggaa cctcctcccg ccgggttacc ggccggggct tcgacgccct cggccgcgag
     7501 cgcccgcaac tcctggccga ccttctctac ctgcgactcg gcctcgcggc ggcgcatggc
     7561 gaggaagctg gagccgccgg cctggttctc cagcacccac tccttcgccc actttccggt
     7621 ctggatgtcg gtgaggatct ggcgcatccg ctgctttgtg ccctcgtcca cgatcttagg
     7681 cccggcggtg tagtcgccgt actcggcggt gttcgagatc gagtagcgca tctgcgccag
     7741 gccgccctcg tagatgaggt ccacgatcag cttcagttcg ttcacgcact cgtagtacgc
     7801 gagctccggc tgataccccg cctcgaccag cgtctcgaac ccggccgtca aaagcgccga
     7861 gagaccgccg cacagcacgg cctgctcccc gaagaggtcc gtctcggtct cctcggcgaa
     7921 cgtggtctcg atgatccccg cccggccgct gccgatgccg cgagcgtacg agaggacgac
     7981 gtcccgcgcc tgtcccgagg cgtcgttttg gacggcgaag agcgacggca tacccttgcc
     8041 ctcgacatag agtctccgca ggacatgacc cggacccttc ggcgcgacga gcccgaggtc
     8101 aacctccggc ggcacgacga tctggttgaa gtggatgttg aagccgtgcg cgaagagcat
     8161 cagcataccc tccgacaggt tcggctccac ctccgcccgg aagaccgccg gctgcttctc
     8221 atccggcagc agcatcatca ccacgtcggc ccactgaacc gcgtccgcga tggtcatgac
     8281 ctgcaggccc tcctgctccg ccttcggcca gctcttactc ccctcgtaga gcccgacggt
     8341 gacttcgcag ccggagtcct tcaggttcag ggcgtgggcg tggccctggc tgccgtagcc
     8401 caggatagcg accttcttct gcgtgatctc gttgccgatg tccgcctcgc ggtatacgcg
     8461 cgccatgcta ttctcctatc tctctacttc tgggccggcc gctcggcgaa ccggatctcc
     8521 tccacgtcct ccaggctttc gaggagcgtg gcgtagcgcc gggccgtctc ctcgggacag
     8581 tcgagcgcga taccgatgcg cgtgccgtca cggtctccgt ccaccgagat cctcgctacc
     8641 ggcatccgct tgttctggag cgtcatcagg aagcggttga gggctaggat gccccccgct
     8701 atccgggcct ctatctctat gggttccacg tcggttgtgg atccagcgga gctaggcgcc
     8761 atcttcctcg atcatctcgg ccacgctcat gcccgaagg
//
LOCUS       DASSMB010000161        20168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1810559, whole genome shotgun sequence.
ACCESSION   DASSMB010000161 DASSMB010000000
VERSION     DASSMB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 20168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20168
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810559"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2811)
                     /gene="carB"
                     /locus_tag="VFJ72_09430"
     CDS             complement(<1..2811)
                     /gene="carB"
                     /locus_tag="VFJ72_09430"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7479721.1"
                     /translation="MPRRDDIESILIIGSGPIVIGQAAEFDYSGTQACRALRDEGYRV
                     ILVNSNPATIMTDPEVADATYVEPLTTGTLAEIIRRERPDALLPTLGGQTALNLAIEL
                     HEAAVLEDYGVELLGASVESIRKAEDRRLFREAMERIGLKVPDSRVVREVSEAEELAE
                     RIGYPLIIRPSFTLGGKGGAVASDLRELRRAVTDGLDASPVRSVLVERSIAGWKEFEL
                     EVMRDLADNVVIICSIENVDPMGVHTGDSITVAPAQTLSDRQYQTLRSASIRIIREIG
                     VQTGGSNIQFAVDPESDEFYVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYALDE
                     IPNDITSATPASFEPALDYVVTKIPRFTFEKFPGATPRLTTRMHSVGEVMAIGRTFTE
                     SLLKAMASLEVEPRDITPNLDEPNPYRIFAIFDALRSGMDIPEIFAKTRIDPFFIASI
                     AKIVAAEGAISGGLSADALLELKRIGLPDEALAAASGVSREVVRGVRHALNIVPTYKS
                     VDTCAGEFPARTPYYYSTYETEDEVERGENASVVVLGSGPNRIGQGVEFDYACVHASY
                     ALKEAGYDAVMVNSNPETVSTDYDTSTRLYFEPLSEEYVLEVLRREKPEGVILQFGGQ
                     SPLKLARVLEKEGFKILGTSPDAIDLAEDRSRFGRLLTELGIPHPRFGTAMSAEEAIR
                     VAEDLGYPVVVRPSYVLGGRRMEIIYNRDDLDLYLRSNADTSPEHPTLIDKFMEDYVE
                     VDVDAVADGEDVYIGGIMEHVEEAGVHSGDSSCVTPPITIHRRLVERIEDYTRRLARA
                     IGVVGLMNVQYVIRGEDVLVIECNPRASRTVPFISKSTGVPLAKVATRVLLGEKLRDM
                     GLRASTNGHFSVKAPVFPFDRFADVDTLLGPEMRSTGEAMGIDATFGGAFAKALTAVG
                     LTLPVAGRVYISVSNRDKRSVVL"
     gene            complement(2813..3940)
                     /gene="carA"
                     /locus_tag="VFJ72_09435"
     CDS             complement(2813..3940)
                     /gene="carA"
                     /locus_tag="VFJ72_09435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7479722.1"
                     /translation="MTGQEQDLEYGPRRATLVLEDGATFEGKTFAGDGEVAGEVVFTT
                     SMVGYQETLTDPSYRGQIVLFTYPLIGNYGVIPGEDESRKVQAAAVLVREYTPYHSNW
                     ASERSLAAMLDEAGVMGIEGLDTRALTRHLRDRGAMRGVISTVERDSNALREKANAHP
                     SMVGLDLASTITELKEPLIMPAFGEERCRIAALDYGVKGSIYKELRSRGATVIALPGS
                     SSSEEVLASEADGLFLSNGPGDPAALQDAVSTLRPVLGELPVFGICLGHQLLGLALGC
                     ETYKMPFGHHGANHPVRNLATGRIEITSQNHGFAVREETLPEGVELTHRNLYDGTVEG
                     IASREARAWSVQYHPESSPGPRDSGYLFDEFVESISEKAQV"
     gene            complement(3903..5198)
                     /locus_tag="VFJ72_09440"
     CDS             complement(3903..5198)
                     /locus_tag="VFJ72_09440"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7479723.1"
                     /translation="MSPELVIRNAHVLDPSIGLDRVTDVRLTDGRVAEIGDGLRGTRE
                     LDADGLHLFPGFVDVHAHWRTPGREDEETIASGSAAAAAGGFTGVVMMPNTDPVVDKP
                     VIVSGLVRRIEQESRVRAYVSAALHAGLEGKRLTEMRLLKEAGAFCVSDDGLGTASAG
                     VLRNGMLYARSAELPVILHCEDHTLATGVVHDGVAAALAGIPGSPASAEDAATATALI
                     LAAETGAKVHITHVSTALSAALVAFFKSWADVTGDTTPHHLTLTDDLVGTLEGMYRVN
                     PPLRPDDDRRGVGDALRDGVLDFVATDHAPHASEEKELPLEEAAPGFLGHETAFAALY
                     SELVLGGVMPLSRLVEAMACAPGRWMGEGAGTLAAGAPADLTLVDLSEEWTVGRENLF
                     SQSANSPYTGRRLTGRVVGTIVGGELIHDRTGARFGVRA"
     gene            complement(5195..6118)
                     /locus_tag="VFJ72_09445"
     CDS             complement(5195..6118)
                     /locus_tag="VFJ72_09445"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET7479724.1"
                     /translation="MRSRDFLTIEGASREELREVISLARAHADGRLSRPLDGKTVCLA
                     FFESSTRTAVSFELAARRCGADVISLSEKGSSISKGESLIDTVVTLDRLGADALVLRH
                     PSAGASALAARYTDAAVINAGDGCGQHPTQALLDLYALSEAVGGFDELEGRRVAISGD
                     ILHSRVARSVIPAFRAAGMEVAIVAPLTLLPQETGAWNVPILDSVDEALEWGAETLYL
                     LRLQNERMNGSSPRVPSVGEYARFYGVARRHLRDGLLVMHPGPVNRGVEIAGDVVLDG
                     ASLVPDQVAAGISVRSAVLTLATGAAHEVAA"
     gene            complement(6108..6662)
                     /gene="pyrR"
                     /locus_tag="VFJ72_09450"
     CDS             complement(6108..6662)
                     /gene="pyrR"
                     /locus_tag="VFJ72_09450"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET7479725.1"
                     /translation="MSHSSTRERPQSQVLTADGLNRSLRRISHEILEKNASDLDGLAL
                     VGVLTRGVPLARRISENIRQFEGLEIPVGSLDITLHRDDLGGDEPQVHRSDIPFDVAG
                     RTVVLVDDVLYTGRTARAAMDALLEMGRPAAIRLAILVDRGHRELPIRADYVGKNVPT
                     ARDERVRVCLTETDGEDGVITVAE"
     gene            complement(6729..7613)
                     /locus_tag="VFJ72_09455"
     CDS             complement(6729..7613)
                     /locus_tag="VFJ72_09455"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7479726.1"
                     /translation="MPSFEVSYGEAGGRLDLLVAQRLGISRSAVQRLISSGSLRVDGE
                     ETSPSHKARAGEKIEVHIPDEDLAAENIAVPIVFEDEHLIVVDKPAGLVVHPGAGNPS
                     GTLVNALLDRGIAGGEDPQRPGIVHRLDRDTSGLMVVAKGEPAYTELVRQLSERRVRR
                     IYRAIVVGEGLPPKGTVDSPVGRNPDNPTLMAAGIGKQAVSHFEVLRQAAGHSMLRVR
                     LETGRTHQIRVHLSAIGYPVYADPLYGEAVPVHRLWLHAGRLAFTHPATGEPLEFVAE
                     IPDDLRRSARELDASFDL"
     gene            complement(7626..7931)
                     /locus_tag="VFJ72_09460"
     CDS             complement(7626..7931)
                     /locus_tag="VFJ72_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET7479727.1"
                     /translation="MAEGFVNQTKDGAQVTLRVSPGARESAVTGTYGESALKVRVAAP
                     PVDGKANEETLRYLAKLLGLPRSRVEILRGSSGRDKVVLVRGVDPEGVRRALSSAIA"
     gene            complement(7924..9021)
                     /locus_tag="VFJ72_09465"
     CDS             complement(7924..9021)
                     /locus_tag="VFJ72_09465"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET7479728.1"
                     /translation="MAIKPIDIRRKEFNNSFRGYDQNGVDDFLDTVADEFERIYTDNV
                     RMRDEIQNVRERLQQFEDLEGSIRSALIHAEQAADDLRKSANREAETLRESAKREAEL
                     TVKEAQNRAHQMLADSSSRVERVQESYEALRHAKQTFAADFRHLLKSYLAVMDNADVA
                     TAKEIEASLRERLDTESVAAAREARAHEGETQHGAPFAETSVQDQETSERQRESDEEE
                     GASMEAVGQEDDRTDDFDEVAAQDDPEATQAFTATEAREATGEPPADAQPETEEPEYE
                     PSSATASTQGTAEQEAVSAQSSASESPAAEEVDREEPGLASQDSSNDFFGEREQAGDQ
                     RREDEPRAEDAPQREEDNRIFRASRFLRRRG"
     gene            complement(9028..9363)
                     /locus_tag="VFJ72_09470"
     CDS             complement(9028..9363)
                     /locus_tag="VFJ72_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7479729.1"
                     /translation="MALLQQFGFSISDLLLGVVNYGYYILLGLILAWILIGWFPSYPS
                     NPIFQAIYDAVKNIVDPIMLPIRSRIPPVRLGGFALDLSPIIALFALSIISSLLGTII
                     DQFLRPVVG"
     gene            complement(9363..10154)
                     /gene="proC"
                     /locus_tag="VFJ72_09475"
     CDS             complement(9363..10154)
                     /gene="proC"
                     /locus_tag="VFJ72_09475"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7479730.1"
                     /translation="MKKVGIIGAGKIGGSLLTRLAESGDYDLGVSDVHAEQLRPYAEK
                     YGMRTTSSNLELAEQSDLLVVAVKPWDVAAVMAQVSPAFNGTEKAVASVAAGVSIATI
                     SENLPPGTAVLRVMPNVCASVGLGSAVVTSNDAGRDTVPVVLEIFGHVGEVMELPERL
                     FDAATALHGSGPAYVALFADALIQAGVREGIPRDVARRLVVGTIEGTAVLLKERSAHQ
                     VRDEVMTPGGTTAAAFVAMEKAGFVGAVYDGVNAAAEQSRGLGKK"
     gene            complement(10169..10747)
                     /locus_tag="VFJ72_09480"
     CDS             complement(10169..10747)
                     /locus_tag="VFJ72_09480"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET7479731.1"
                     /translation="MGVRDQLERVAAWFGFGVEDDDYYEDEEEETGRYGGAYSNGARD
                     AEPGPAVRRLGRAERSSAFGTSLGDLFGSEGPSRERYGSQGQQSAHLRAVPDHSPAPA
                     RVSVLEPSSFNDAQALADRFKRQQPVILNLQNVDGDLSRRMVDFCAGLTYALDGQIQT
                     VANRVFLLTPRNVEVSAEERKRLAERAFFNQL"
     gene            complement(10757..11464)
                     /locus_tag="VFJ72_09485"
     CDS             complement(10757..11464)
                     /locus_tag="VFJ72_09485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7479732.1"
                     /translation="MKTRISDEKIQERLGAVRERVGQALERSGRRPGETRILIACKYY
                     EPEQIAALAAAGVELLGENRAEDLIRKQEAFGEVFEWHFIGHLQRRKARDVAGRVSLI
                     HSVDSLRLVEELAKRAPQDGVDVLLQVNVSGEESKYGVREDGIEGLLEAAANTRGKVR
                     ARGFMTIAPMVEDPENVRYVFAKLRTIRDRLQADWAPHFDLSELSMGMSGDYQVAVEE
                     GATLVRIGRTIIEEDGP"
     gene            complement(11461..12150)
                     /locus_tag="VFJ72_09490"
     CDS             complement(11461..12150)
                     /locus_tag="VFJ72_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HET7479733.1"
                     /translation="MSPEPEPPDARIRFFTRLGGVSEPPFDSLNVSTKVGDGEAAVFE
                     NLSRTRAAMGGGESAWVRQVAGADVVRVSGPGFAGEADALHTDHAGFSLVVAVADCVP
                     VALVGERGVGMVHSGWRGTLAGISGKAVREIQGGTIRAFIGPCIRECCYEVSEELAEK
                     FGEAFGAAVVSGWNLSLPAAIREDLRRAGVEEVFDLGLCTGCRTDLFYSHRKEGPRTG
                     RNLAAISWETP"
     gene            complement(12255..13319)
                     /gene="ftsZ"
                     /locus_tag="VFJ72_09495"
     CDS             complement(12255..13319)
                     /gene="ftsZ"
                     /locus_tag="VFJ72_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET7479734.1"
                     /translation="MLDAGTNYLAVIKVVGIGGGGTNAVNRMINSGLQGVEFIAVNTD
                     AQALQMCDADQKIHIGEKLTRGLGAGADPKIGMEAAEENKAEIEESLRGADMVFVTAG
                     KGGGTGTGAAPVVAKIARDSGALTVGVVTRPFAFEGRRRATYAEEGIKRLKENVDSLI
                     IIPNDRLLQVAEKRTSMMDAFKMADDILRKGVQGITDLITVPGLINLDFADVRTIMNN
                     SGSALMGIGESSSENRGAEAAKTAISSPLLEASIEGATGIILNITGGTDLGLFEVNEA
                     AEIVHNAAHQDANLIFGAVVDDSYGEKVSVTVIATGFDQRLVNQRRSERPVMEQQPEE
                     TSSPTQEGDVLDIPAFLRRR"
     gene            complement(13355..14566)
                     /gene="ftsA"
                     /locus_tag="VFJ72_09500"
     CDS             complement(13355..14566)
                     /gene="ftsA"
                     /locus_tag="VFJ72_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET7479735.1"
                     /translation="MATSLVYGIDVGTTKIVAIAGRVDSRRGWVEVLSMGEAPSHGLK
                     RGVVVDRQAAAESVAEAIEACGMRGVPVNVGIAGSHISSFNTEVTLLNRGRDLTITER
                     FVKKLEQEARQIDLTEDERIIHVVPRGFVLDGSEGVKQPVGLAARKVTMRAHVVSGAL
                     SSIQNLLHAVEDCGVKVTQVVLEPLASAESCLLEQDRSDGVALLDMGGGTTDVAAFMR
                     GALTHTAVIPLGGESFSSDLAYGLKVPYDAAEALKVRYGTVLSSSVDSVAAVRLGDRH
                     YNANFMSQILEYRAREILEYTRESLDKAHVRNLLPQGVVLTGGGSLLDGMTELAEDVL
                     GMRARTAVPRRVKGNVEPVQKPQYATAVGLLYFSAKNSHVAPKSKGAGMTSFGSIVEA
                     VKGWFRGGRAG"
     gene            complement(14576..15268)
                     /locus_tag="VFJ72_09505"
     CDS             complement(14576..15268)
                     /locus_tag="VFJ72_09505"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET7479736.1"
                     /translation="MPGTLRRVVRGISLSIAVAGLTGAVVFIVAYLLYPVTGIQVKGA
                     RMYPESEVSQSLSDGASLLTLNTAMLRRQVETNPWVESAKVTKQWQSGIVIVQVKERR
                     AVLDGDLDGRRVILAVDGTELPGLGGARLGRVKVDEDQVEEILRAGRSLESHGIALQS
                     VDDVNAGGTEATVEGRRIIFADRLADDQIEALPEIMRSNPRAPLFDLRSPGRVVVSYE
                     DPPSSRDGGTSG"
     gene            complement(15271..16608)
                     /gene="murC"
                     /locus_tag="VFJ72_09510"
     CDS             complement(15271..16608)
                     /gene="murC"
                     /locus_tag="VFJ72_09510"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7479737.1"
                     /translation="MRIHMIGIGGAGMSGIAEILQKRGHAVTGSDLKESAYTRRLKET
                     GMRVYIGHASDQVGDAEQVVISTAIPPTNLELLEARRRSIPVIPRAAALATILQGGQS
                     IAVAGTHGKTTTTSMTTHVLRSLGESPTALVGGELNDIGSNVVEGSPDLVVAEADESD
                     RSLLHLRPHNAIITNVEFDHPDFYSSLEDVLDTFSKFVAALPEDGHLVTCAEDPSCLQ
                     LASSAPCSVTTYGITAGDLRAVVLGPNSYTLVEDGQERGLIELGVFGRHNILNSLAAV
                     GIARRLGHDALDAARTLKDFGGVRRRFQFKGERGEIRVVDDYAHHPTELLATLDVARA
                     TSKGRVIAVFQPHRYSRTRTLHREFGEAFTAADAVLITDVYGAGEMPQPGVSGKLIVD
                     AICETREHPDVYYIPEQAAIPNVLRSISNRGDTVLTMGAGDISRVGEELLELL"
     gene            complement(16605..17609)
                     /locus_tag="VFJ72_09515"
     CDS             complement(16605..17609)
                     /locus_tag="VFJ72_09515"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET7479738.1"
                     /translation="MADSLRASGATVEFVGAESGIERDLVPQAGYKLHSLPLKGLAGN
                     PLARARAATLFLKALHSCRRIIQNFEPGAVLGVGGYASAPAVIAARSLGIPTFLHEQN
                     SVPGRVNRFASRLTREVLVTFPAAKERLRDAVWVGMPTRREFFGASREEALNDLGLEP
                     PVVVIFGGSGGALKINLAAAEAFRGETPYTVVQISGRRDFPRLSTDNPRHRILEYVDD
                     IWRYVTAADVVVIRSGAGSLFDTAAAGRAAILIPFPYATGDHQLHNARYFTENGAAEL
                     MLDSEVNARTLRERIEDLLEDDERRDRLAARMRGLATPGAADEVAGRLLAATREDGRR
                     "
     gene            complement(17599..18747)
                     /locus_tag="VFJ72_09520"
     CDS             complement(17599..18747)
                     /locus_tag="VFJ72_09520"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HET7479739.1"
                     /translation="MASLRTNLFLVTLGLSLLGMVMVYSATYRDYGPHFLFVRLWHVT
                     LGVIVFLLVSRVRYTSWRKFAPWLYVAVLALLVLVLVPGIGVEAGGARRWLDLGIFSL
                     QPAEFAKLAAVVALSCAVARMPSGSGIPFRPLAAVGALFGLVLVEPDFGTSVVLLAGV
                     AGVLWASEVRTRDLLMAGGAAVVALVGVMWLEPYRRQRFMTFLDPWSVSDDSGYQIVQ
                     SLVAIRTGSLFGTGAGSGGQAATVPEIQTDMIFALIGEEFGLFGMMAVIAALCFVLLA
                     GLRIAFHAPSVMARCMAAGLTTMIAVQATFNIGAAMGVLPLAGMTLPFISYGGSSLIV
                     CFAAVGILYRISEDSERVREIKPKKSAARPDSRRGYGRTRDSRALRGG"
     gene            complement(18747..20009)
                     /gene="murD"
                     /locus_tag="VFJ72_09525"
                     /pseudo
     CDS             complement(18747..20009)
                     /gene="murD"
                     /locus_tag="VFJ72_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
BASE COUNT         3403 a         6982 c         6210 g         3573 t
ORIGIN      
        1 caacacgacc gaccgcttgt cccggttgga gacggagatg tacacccgcc ccgccaccgg
       61 gagcgtcagc cccaccgccg ttagcgcctt ggcgaacgcc ccgccaaagg tagcgtctat
      121 ccccatcgcc tcgcccgtcg aacgcatctc cggccccagc aacgtgtcca cgtccgcgaa
      181 ccggtcgaac gggaagaccg gcgccttcac cgagaagtga ccgttggtgg aagcgcgcag
      241 gcccatatcc cgcagtttct cgcccaagag cacccgcgta gccactttcg ccagcggaac
      301 cccggtggac ttggagataa acggcacggt gcgcgacgcc cgcgggttac actcgatgac
      361 cagcacgtcc tcgccgcgta tcacgtactg cacgttcatc aggcccacga cacctattgc
      421 ccgcgccagc cgccgcgtgt agtcctcgat cctctcgacg agccgccggt ggatcgtgat
      481 cggcggcgtc acgcacgagg agtcgcccga atgcaccccc gcctcctcga cgtgctccat
      541 gatcccgcca atgtacacgt cctcgccgtc ggcgacggcg tcgacgtcaa cctctacgta
      601 atcttccatg aacttgtcga tgagggttgg gtgctccggg ctggtgtccg cattcgacct
      661 tagatatagg tccagatcgt cccggttgta gatgatctcc atccttcggc cgccgagcac
      721 gtaggaggga cgcactacca ccggataacc gaggtcttct gccacccgaa tggcctcctc
      781 cgccgacatc gcggtcccga agcgcgggtg cgggatgccc agttccgtca gcaggcgccc
      841 gaacctggag cggtcctcgg caaggtctat cgcgtccggc gacgtaccca gtatcttgaa
      901 gccctctttc tcgagaactc gcgctagctt gagcgggctc tgccccccga actggaggat
      961 gaccccctcg ggtttttcac ggcgcaacac ctccaacacg tactcctccg agagcggctc
     1021 gaagtacagc cgggtggaag tgtcgtagtc ggtcgagacc gtctcggggt tggagttgac
     1081 catgaccgcg tcgtagccgg cttccttgag cgcgtagctc gcgtggacgc aggcataatc
     1141 gaactctacg ccctgtccga tccggttggg accgctgccg agaacgacca cggaggcgtt
     1201 ctcaccccgc tcgacctcgt cctcggtttc gtatgtggag tagtagtagg gcgtgcgagc
     1261 cggaaactcg ccggcgcatg tatcgacgga tttataggtc ggcacgatgt tcagcgcgtg
     1321 ccggacgcct cgcaccactt cacgggatac accggaagcc gcggccagag cctcgtccgg
     1381 gagcccgata cgcttcagtt ccagcagggc gtcggcgctg aggccgccgg agatagcgcc
     1441 ctcggcggcc actatcttgg ctatggaggc gataaagaac gggtcgatgc gggtcttggc
     1501 gaagatctcg ggtatatcca tcccgctcct tagagcgtcg aagatagcga agatcctgta
     1561 cgggttgggc tcgtccaggt tgggggttat gtctcgcggc tctacctcca gtgaagccat
     1621 agccttgagc aggctctccg tgaacgtccg ccctatagcc atgacctcgc cgacggagtg
     1681 catcctggtc gtgagccggg gcgtggcgcc ggggaacttc tcgaaagtaa agcgcggtat
     1741 cttggtaacg acgtagtcga gagccggctc gaaggacgcg ggtgtggcgc tggtgatgtc
     1801 gttcgggatc tcatcgagag cgtacccgac cgccagcttg gccgctattt tggcaattgg
     1861 aaagccggtc gccttgctcg ccagcgcgct ggagcgggag acgcgcgggt tcatctcgat
     1921 cacgtagaac tcgtcgctct ccgggtcaac ggcgaactgg atgttcgacc cgcccgtctg
     1981 aaccccgatc tcgcgtatta tgcggataga ggcgctgcgg agggtctggt attggcggtc
     2041 ggagagggtt tgtgcggggg cgacggttat ggagtcgccg gtgtggacgc ccatcgggtc
     2101 tacgttctct atggagcaga tgatcacgac gttgtccgcc aggtcgcgca tgacctccag
     2161 ctcgaactct ttccagccgg cgatactacg ctcgacgagc acgctccgca cggggctcgc
     2221 gtcgagaccg tccgtgacgg cgcggcgcag ctcgcgcagg tcgctcgcga cggcaccgcc
     2281 cttgccgccg agggtaaagc tcggacggat gatgagcgga taaccgatgc gctcggccag
     2341 ttcttcagct tcggaaactt cgcgcacgac gcggctgtcc ggaaccttta gcccgatacg
     2401 ctccatcgct tcccggaaca ggcggcggtc ctccgccttc cgtattgact ccacggacgc
     2461 tccgagcaac tcgacgccgt agtcctccag gaccgccgcc tcgtgcagct cgatggcgag
     2521 gttgagggcg gtctggccgc cgagggtcgg gaggagggcg tcggggcgtt cgcggcgtat
     2581 gatctcggct agcgtgccgg tggtgagggg ttcgacgtag gtagcgtcgg cgacctccgg
     2641 gtcggtcatt atggtagccg ggttggagtt gaccagtatc acgcggtatc cctcgtcgcg
     2701 cagggcgcga caggcttgtg tccccgagta gtcgaattcg gcggcctgac cgatcactat
     2761 aggcccggag ccgatgatca ggatgctctc tatgtcgtcg cgccgcggca ctttagacct
     2821 gggctttctc ggagatgctc tccacgaact cgtcgaacag gtatccggag tctcgcggtc
     2881 cgggactcga ctcggggtga tactggacgc tccaggcgcg tgcctcccgg ctcgcaatgc
     2941 cctcgaccgt gccgtcgtag aggttgcggt gcgtcagttc cacgccctcc ggcagcgtct
     3001 cctcccggac ggcgaagccg tggttctggc tggtgatctc gatgcgcccc gtcgccaggt
     3061 tgcggacggg atggttggct ccgtggtgtc cgaacggcat cttgtacgtc tcgcaaccga
     3121 gcgcgagccc gagtagctgg tgtcccaggc agatgccgaa gaccggcagt tctccgagga
     3181 cgggacgcag ggtgctcacg gcgtcctgca gagctgccgg gtcgccgggt ccgtttgaga
     3241 ggaagaggcc gtctgcctcc gaagccagca cctcctccga agacgaggag cccggcagcg
     3301 cgatgaccgt tgcgccccgg ctgcgaagct ccttgtagat ggagcccttc actccgtagt
     3361 cgagcgcggc gatgcggcac cgctcctcac cgaaggccgg catgataagc ggttccttaa
     3421 gctcggtgat ggtggaggcg agatcgagtc cgaccatcga ggggtgcgcg ttggctttct
     3481 ctcgcagcgc gttcgagtca cgctcaacgg tcgagatgac gccccgcatc gcgccccggt
     3541 cgcgcaggtg gcgggtgaga gcgcgcgtat ccagaccttc gatgcccatt acgccagcct
     3601 cgtccagcat ggcggccagc gagcgctcgc tggcccagtt gctgtgatac ggggtgtact
     3661 cacgcacgag gacagcggcg gcctgcacct tgcgcgactc gtcctcaccg gggataacgc
     3721 cgtagttccc gatcaggggg taggtgaaca gcacgatctg tccgcggtac gatggatcgg
     3781 tcagcgtctc ctgatagccg accatgctgg tcgtgaagac cacctcgccc gcgacctcgc
     3841 cgtctccggc gaaggtctta ccctcaaaag tagccccgtc ctcgaggacg agggttgccc
     3901 gtctaggccc gtactccaaa tcttgctcct gtcctgtcat gaataagctc tccgccaact
     3961 atagtaccga cgaccctgcc cgtcagcctg cgtccggtat acggcgagtt ggccgactgg
     4021 ctgaaaaggt tctcccgccc gaccgtccac tcctccgaca ggtccacgag cgtcaagtcc
     4081 gccggcgctc ctgcggcgag agtacccgcg ccttcgccca tccaccgtcc cggcgcgcag
     4141 gccatcgcct ctaccagccg cgagagaggc ataactccac ccaggaccag ttcgctgtac
     4201 agcgccgcaa aggccgtctc gtgaccgagg aaccccggcg cggcctcctc cagtggaagc
     4261 tccttctcct cggaagcgtg cggcgcatgg tcggtggcga cgaaatctaa tactccgtcc
     4321 cgcaacgcat ccccgacacc ccggcggtcg tcgtccgggc gcagcggtgg attgacgcga
     4381 tacatacctt ccagcgttcc aacgaggtcg tcggtgagcg tcaggtggtg cggcgtggtg
     4441 tctccggtaa cgtcggccca gctcttgaag aacgcgacca gcgccgccga tagtgcggtc
     4501 gagacgtggg taatatggac cttcgcgccc gtctcggcgg ccaggatcag ggccgtcgcc
     4561 gtcgccgcgt cctcggcgct cgcgggggag ccgggaatac cggccagagc cgccgcgacg
     4621 ccgtcgtgga cgacgccggt ggcgagggta tggtcctcgc agtgcaggat cacgggcagc
     4681 tccgccgacc gggcgtagag catgccgtta cgcaggacgc cagccgaggc cgtgcccagc
     4741 ccgtcgtccg agacgcagaa cgctccggcc tccttcaaca gccgcatctc cgtgaggcgt
     4801 ttcccttcca ggccggcgtg cagggcggcc gagacatagg cccggacacg ggattcctgc
     4861 tcgatgcggc gcacaaggcc gctcacgatc acgggcttgt ccaccaccgg gtccgtgttc
     4921 ggcatcatga cgacgccggt gaacccaccg gcggccgccg ccgcggaacc gctcgctatc
     4981 gtctcctcgt cctcccggcc cggcgtgcgc cagtgggcgt ggacgtccac gaagccgggg
     5041 aagaggtgca gcccgtcggc gtccaattct cgtgtgccgc gaagaccgtc cccaatctcc
     5101 gcgacgcggc catctgtcag acgcacgtcg gtcacgcggt ctaggccgat tgaggggtcg
     5161 aggacgtggg cgttgcggat aacgagttcg gggctcacgc ggctacctcg tgggcggctc
     5221 ccgtggcgag ggtgaggacc gccgagcgga cggagatacc ggcggcgacc tgatccggga
     5281 ccagcgaagc cccgtcgagc accacatcac ccgcaatctc cactccccta ttgaccggac
     5341 cgggatgcat gacaagcaaa ccgtcgcgca aatgccgccg cgctacgccg tagaacctcg
     5401 cgtactcacc caccgacggg acacgcggcg agcttccgtt catccgctcg ttctgaaggc
     5461 gcagcaggta cagggtctcc gcgccccatt ccagcgcttc gtctacggaa tcgaggatcg
     5521 gcacgttcca ggctccggtc tcctggggca gcagcgtcag cggcgcgacg atggcgacct
     5581 ccatgcccgc tgcgcggaac gcggggatca cgctgcgcgc cacccggctg tgcaggatgt
     5641 ctccggatat cgctaccctg cggccctcaa gctcatcaaa gcctccgacg gcttcgctca
     5701 gggcgtacag gtcgagcagc gcctgggtcg ggtgctggcc gcagccgtcg cccgcgttga
     5761 taacggccgc gtccgtatag cgcgccgcga gagccgaggc tccggcggac gggtggcgaa
     5821 ggacgagggc gtcggcgccg agccggtcga gggtgacgac ggtgtcaatc agggactcgc
     5881 ccttagagat ggatgagcct ttctcggaga gcgagatcac gtccgcaccg caccggcggg
     5941 cggccagttc gaaggagacg gccgtgcggg tcgaggactc gaagaaggcg agacaaacgg
     6001 tcttgccgtc cagggggcgc gagaggcgtc catcggcgtg ggcgcgggcg agcgagatca
     6061 cctcccgcag ttcctcccgg ctggcgcctt ctatcgtcag gaagtctcta ctccgcaacg
     6121 gtgatcaccc catcctcccc atcagtctcg gtcaggcaga cgcgaacccg ctcgtcacgg
     6181 gcggtcggca cattcttgcc cacgtaatcg gcccggatcg gcaactcgcg gtggccccgg
     6241 tcaacgagaa tcgccagccg gatcgccgcc ggcctcccca tctccaggag cgcgtccatc
     6301 gccgcccgcg ccgtgcgtcc cgtgtacagc acgtcgtcca cgagcacgac cgtgcgtccg
     6361 gcaacgtcga acggtatgtc gctccgatgt acctgcggtt cgtcgccgcc aaggtcatcc
     6421 cggtgaagcg tgatgtccag cgacccgacc gggatctcca gcccctcgaa ctggcggatg
     6481 ttctcggaga tcctgcgcgc cagcgggacc ccgcgcgtca acacgccgac gagcgccagc
     6541 ccgtcaaggt cggatgcgtt tttttcgagt atctcgtggg atattcgccg gagcgaacgg
     6601 ttcagcccgt ccgcggtgag cacctgcgac tgcggacgct cgcgcgtgct tgaatgactc
     6661 aagcccttct tcctccttcg cgcctcacgg gacgccgtta aaggatcggt atgtcgccag
     6721 tcaagctatc agaggtcgaa ggatgcgtca agctcgcgcg ccgaccggcg gaggtcatcc
     6781 ggtatctccg ccacgaactc cagcggctct cccgtggccg ggtgcgtgaa cgcgagccgc
     6841 ccggcgtgga gccagagccg gtgtacgggg acggcttcgc cgtagagtgg gtccgcgtag
     6901 acagggtacc cgatggcgga gagatggacc cggatctgat gcgtcctccc ggtctccaat
     6961 cgcacccgga gcatcgagtg ccccgccgcc tgccgcaaga cctcgaagtg gctcacggcc
     7021 tgcttcccga taccggccgc cataagcgtg gggttatcgg ggttgcgtcc gaccggcgag
     7081 tccacagtac cctttggcgg aagcccctcg ccaacgacta tagcccggta gatcctccgc
     7141 acccgccgct ccgagagctg cctcaccaac tccgtatacg cgggctctcc cttggccacc
     7201 accatcagac cggaggtatc gcggtcgagc cggtgtacga tgccgggccg ctgcgggtct
     7261 tctccgcccg ctatcccccg gtcgagaagc gcgtttacca gagtcccgga tgggttacct
     7321 gctcccggat gcacgacaag tccggcgggc ttgtccacga cgatcaggtg ctcgtcctcg
     7381 aagactatcg ggacggcaat attttcagcg gcgaggtctt cgtcggggat gtggacttcg
     7441 atcttctcgc ccgcgcgagc cttgtgagat ggcgaggtct cctctccgtc cacacgcagc
     7501 gagccggaag agatcaaccg ctgcacggcg ctccggctga tgccgaggcg ttgcgccacg
     7561 aggagatcca gccgcccccc cgcctcgccg tacgatacct cgaagctcgg cacgctcaag
     7621 cgcggttacg cgattgcgct ggagagggcc cgacggacac cttcgggatc cacgccgcga
     7681 acgagtacga ccttgtcgcg tccggatgag ccgcgcagga tctcgaccct cgacctcggt
     7741 aacccgagta gcttcgccag atatcgaagc gtctcctcgt tggccttgcc atccaccggg
     7801 ggagcggcga cgcgcacttt caacgcgctc tcgccataag tccccgtcac ggcgctctct
     7861 cgggctcccg gcgagacccg gagcgtgact tgcgcgccgt ccttcgtctg gttgacgaac
     7921 ccctcagcca cgccggcgga ggaagcggct ggcgcggaag atcctgttgt cctcctcacg
     7981 ctgcggcgcg tcttcggcgc gcggctcgtc ctctcgcctc tgatccccag cctgctcgcg
     8041 ctccccgaag aagtcgttgg agctgtcctg agaggcgagg cctggctcct cccggtctac
     8101 ctcctctgcc gccggcgact cgctcgccga agattgcgcg gagacggcct cctgctccgc
     8161 cgtaccttgc gtgctggcag tcgcgctgga gggctcgtac tcgggttcct cggtctccgg
     8221 ctgtgcatcg gctggcggct cgcccgtggc ctcgcgagcc tcggtcgccg tgaatgcctg
     8281 cgtcgcctcc gggtcgtcct gcgcggcaac ctcgtcaaaa tcgtccgtgc ggtcgtcctc
     8341 ctgtcccacg gcttccatcg aggcgccttc ctcttcgtca ctctcgcgct ggcgctcgga
     8401 ggtctcctgg tcctgcactg acgtttccgc gaacggcgcg ccatgctgcg tctcgccctc
     8461 gtgcgcccgg gcctcacggg cggcggccac agactcggta tccagccgct cgcgcaggga
     8521 ggcttctatc tccttggcgg ttgcgacgtc agcgttgtcc attacggcga ggtagctctt
     8581 taggaggtgc cgaaagtccg cggcgaaggt ctgcttcgcg tgcctcaggg cctcgtagga
     8641 ctcctgcacc cgctcgaccc ggcttgagga gtcggccagc atctgatggg cacggttctg
     8701 agcctccttc acggtcagct cggcctcgcg cttcgcgctc tcccggaggg tctccgcctc
     8761 gcggttcgcg ctctttcgca ggtcgtccgc cgcctgctcc gcgtgtatga gcgccgagcg
     8821 tatcgaacct tccaggtcct cgaactgctg cagccgttcg cggacgttct ggatctcatc
     8881 gcgcatccgc acgttgtccg tgtagatgcg ctcgaactca tctgccaccg tatccagaaa
     8941 gtcgtctacc ccattctggt catagccccg gaagctgttg ttgaactctt tacgtcgaat
     9001 atctatcggc ttgatcgcca cgatgaatca ccccactaca ggtcgtagaa actggtcaat
     9061 gattgtgcca agcaggctgc taattatgga caacgcgaac aacgcgatta taggcgaaag
     9121 gtccagcgcg aacccgccaa gcctcaccgg cgggatgcgg ctgcggatcg gcagcattat
     9181 cggatccacg atgttcttga cggcgtcgta gatcgcctgg aagatcgggt tggacgggta
     9241 ggacgggaac cagccgatca ggatccaggc cagtatcaac ccgagcagta tgtaataccc
     9301 gtagttgacg accccgagca acagatccga gatgctgaag ccgaactgct gcaggagagc
     9361 catcactttt tacccagccc cctcgattgc tccgcagccg cgttgacgcc atcgtacacc
     9421 gcaccgacga acccggcctt ctccatcgcg acgaacgccg ccgcagtcgt tccgcccggc
     9481 gtcatcacct cgtcgcggac ctgatgcgcg ctgcgttcct tgagcagcac ggctgtcccc
     9541 tctatcgtcc cgacgacgag cctgcgcgcc acgtcgcgcg ggatgccctc ccgcacgcca
     9601 gcctggataa gcgcgtcggc gaagagcgcc acgtacgccg ggcccgaacc gtgcagcgcg
     9661 gtcgccgcgt cgaacagacg ctccggcaac tccatcacct ctccgacgtg gccgaagatc
     9721 tccagcacca ccggcaccgt atccctgccg gcgtcgttcg acgtcaccac cgccgagccc
     9781 agcccgaccg aggcgcagac gttcggcata acgcgcagga ccgccgtgcc gggcggcagg
     9841 ttctccgaga tagtcgcgat cgagacgccc gccgcgacgc tcgccacggc tttctccgta
     9901 ccgttgaagg ccggtgaaac ctgagccatg accgccgcca cgtcccacgg tttcacggct
     9961 acgacgagca ggtcgctctg ctcggcgagc tcaaggtttg aagaagtggt ccgcatgccg
    10021 tacttctcgg cgtagggcct gagctgttcg gcgtggacat cgctcacccc gaggtcgtaa
    10081 tcgcccgact cggcaagccg ggtgagcagc gagcccccga tcttacccgc cccgattatc
    10141 cctaccttct tcaagtttcc cacgagatct agagttggtt gaagaacgcc cgctcggcga
    10201 gacgcttgcg ctcttcggcg ctcacctcga cgttgcgggg ggtcaggagg aaaacccggt
    10261 tggcgacggt ctggatctgc ccgtccagcg cgtatgtcaa accggcgcag aagtccacca
    10321 tccgccggct gaggtcgccg tccacgttct ggaggttcag gatcaccggc tgctgccgct
    10381 tgaacctgtc cgcgagcgcc tgcgcgtcgt tgaagctcga cggctccaac acactcaccc
    10441 gcgcgggcgc ggggctgtgg tccggcacgg cgcgcaggtg cgcggactgc tggccctgac
    10501 ttccgtacct ctcccggctc ggtccctcgc taccgaagag gtccccgagc gaggtgccga
    10561 acgccgacga gcgctccgcc cgtcccagac gccggacggc ggggccgggc tcggcatccc
    10621 gcgctccgtt ggagtacgcg ccgccataac ggcccgtctc ctcttcctcg tcctcatagt
    10681 agtcgtcgtc ctcaacacca aacccgaacc aggccgcgac ccgctctagc tgatctctaa
    10741 ctcccatggt gcctcctcac gggccatcct cctcgataat cgttcgcccg atccgtacta
    10801 aagtcgcccc ctcttcaacc gcaacttggt agtcgccgct cattcccatc gagagttctg
    10861 aaaggtcgaa atgcggagcc caatcggcct gtagcctatc acgtatcgtc ctcagtttcg
    10921 caaaaacata gcgcacattt tccgggtctt caaccatcgg cgctatggtc atgaaacccc
    10981 tcgcccggac cttgcccctt gtattggcgg cagcctcgag caggccctca atgccgtcct
    11041 cccgtacccc atacttcgat tcctcaccgc taacgttcac ctgcaacagc acgtccacgc
    11101 cgtcctgtgg cgcgcgtttt gccagctcct cgaccagccg gagcgagtca accgagtgga
    11161 tgaggctcac tctgcccgcg acgtctctcg ccttgcggcg ctgaagatga ccgataaagt
    11221 gccactcgaa aacctcccca aaggcttcct gcttgcggat caaatcttcc gcccggttct
    11281 cgcctagtag ctcgacgccg gctgcggcga gcgccgcgat ctgctcgggt tcgtagtatt
    11341 tgcaggctat gaggatcctg gtctcgcccg gccgtctacc gcttcgctct agcgcctgcc
    11401 ccacccgctc gcgcacagcc ccgagccgct cctgtatctt ttcgtccgaa attctggtct
    11461 tcacggagtc tcccacgaaa ttgccgccag gttcctgccg gtgcgcggcc cctccttgcg
    11521 gtgcgaatag aagaggtccg tccggcagcc cgtgcagagc cccaggtcga agacctcctc
    11581 tacacccgct cgccgcaagt cttctcgaat cgccgccggc agtgagagat tccatcccga
    11641 gaccacagcc gccccgaacg cttcgccgaa cttctccgcc aactcctcgg atacttcgta
    11701 gcagcattcc cggatgcatg gcccgatgaa cgctcgaatc gtcccgccct gtatctcccg
    11761 cacggcctta ccggagatac cggccagcgt gccgcgccac ccggagtgga ccatccccac
    11821 gccgcgctct cccacgagcg ccacgggcac gcagtccgcg acggccacga ccagggagaa
    11881 ccccgcgtga tccgtgtgca gcgcgtccgc ctccccggcg aaccccggcc ccgagacgcg
    11941 aaccacatcc gcgccggcca cctgacggac ccacgcgctc tccccgccgc ccatcgcggc
    12001 tcgcgtgcgt gagaggttct cgaagacggc ggcctcgcca tcaccgacct ttgtcgagac
    12061 gttgaggctg tcgaacggcg gctcggagac accgcccagg cgggtgaaga accggatacg
    12121 cgcgtccggc ggctcaggtt ccggggacac atagacggcg ccgccggtgg cggtccgaac
    12181 cgtccacccg gcggcgccgg aagagggatg atctaagcgg ggtgagttct gatacacgcc
    12241 ctggcggtct cggcctagcg gcgtcggagg aaggccggga tgtccaggac atcgccttcc
    12301 tgcgtcgggc tgctcgtctc ctctggttgc tgctccatca ccggacgctc cgagcgtcgt
    12361 tgattgacga gccgctggtc gaagcccgtg gcgatcaccg tgacgcttac cttctcgccg
    12421 tacgaatcgt ccaccacggc gccgaagatc aggttcgcgt cctggtgcgc cgcgttgtga
    12481 acgatctccg cggcctcgtt cacctcgaac agtcccaggt ccgttccgcc cgtgatgttc
    12541 aatatgatcc cggtagcgcc ctcgatgctc gcctccagca gcgggctcga tatcgccgtc
    12601 ttggcagcct cggccccgcg gttctcgctg ctcgactcgc cgatacccat gagcgccgag
    12661 ccggagttgt tcatgatcgt gcgcacgtcc gcgaagtcga ggttgatgag gcccggtact
    12721 gtgatgaggt ccgtgatgcc ctggacaccc ttcctgagta tgtcgtcggc catcttgaag
    12781 gcgtccatca tgctggtccg cttctcggcg acctgcagca gccggtcgtt cgggatgatg
    12841 atcagagagt caacgttctc cttgaggcgc ttgatccctt cctcggcata cgtcgcccgg
    12901 cggcgaccct cgaacgcgaa cggccgcgtg acgacaccca cggtcagagc gccggagtcg
    12961 cgggcgatct tggctacaac aggagcggcg cccgtcccgg tgccgccacc cttgcccgca
    13021 gtcacgaaca ccatgtccgc cccgcgcagg gactcctcga tctctgcctt gttctcttcg
    13081 gccgcctcca tgccgatctt ggggtccgcg ccggctccga ggcccctagt aagtttctcc
    13141 ccaatgtgta tcttctggtc cgcatcacac atctggagag cctgcgcgtc cgtgttcacc
    13201 gcaatgaact caaccccctg caacccggag ttgatcatgc ggttcacagc gttggtcccg
    13261 ccgccgccga tgccgacaac ttttattacc gccagatagt tcgttcccgc gtccaacata
    13321 cccttctagc cctccattta ccgttctcct gccgtcatcc ggcgcgtcct ccccggaacc
    13381 aacctttgac cgcctccact atgctaccaa aactggtcat ccctgcacct ttactctttg
    13441 gtgcaacgtg actgtttttc gcggagaagt atagcagacc tacagccgtt gcatactgag
    13501 gcttttgtac aggttcaacg ttgcccttta cgcggcgtgg cacggcggtg cgagcgcgca
    13561 tacccaggac atcctcggcc agctccgtca tgccgtcgag gagggagcct ccgccggtaa
    13621 ggacgacgcc ctgaggaagg aggtttcgga cgtgggcctt gtccagcgat tcgcgggtgt
    13681 actccaggat ctcgcgagcg cggtactcca ggatctggct catgaaattc gcgttgtagt
    13741 ggcggtcgcc aagtctcaca gcggcgacgg agtcaacgga actggatagg acagtcccgt
    13801 agcgtacctt gagcgcctcg gcggcatcgt atgggacctt gagtccgtag gcgaggtcgg
    13861 aggagaagct ctcgccgccg agagggatga cggcggtgtg ggtcagggcg ccgcgcatga
    13921 atgccgctac gtccgtggta ccgccaccca tgtccaggag ggcgacgccg tcggagcggt
    13981 cctgctccag aaggcaggac tccgcggagg ccagaggctc cagcacgacc tgcgtcacct
    14041 tcacgccgca gtcctcgacg gcatgcagga ggttctggat gctggagaga gcgccgctga
    14101 cgacgtgcgc gcgcatcgtt accttgcggg ccgccaggcc cacgggctgc ttcacgccct
    14161 cggagccatc gaggacgaac ccgcgaggca cgacgtggat aatgcgctcg tcctcggtca
    14221 ggtcgatctg ccgggcttcc tgctccagct tcttcacgaa gcgctcggtg atcgtcaggt
    14281 cccggccacg gttgagtagc gtgacctccg tgttgaaaga ggagatgtgg ctgccggcga
    14341 tcccgacgtt gaccggcacg cctcgcatgc cgcacgcctc gatggcctcc gccaccgact
    14401 ccgcggccgc ctgccggtcc accaccacgc cacgcttgag gccatgacta ggagcctcgc
    14461 ccatcgagag gacctcgacc cagccgcggc ggctgtccac ccgtccggcg atggcgacga
    14521 tcttcgtcgt ccccacgtcg atcccataga ctagcgaggt tgccacgctg ccgcgctacc
    14581 cgctcgttcc gccgtcccgg ctggaaggcg ggtcctcgta actgaccacg accctccccg
    14641 gcgaacgaag gtcgaaaagc ggagcccgag ggttgctgcg cattatctcc gggagagctt
    14701 cgatctggtc gtcagccagc cgatccgcga agatgatcct gcgcccctcg accgtcgcct
    14761 cggtaccgcc cgcgttcacg tcgtcaaccg actgcagagc aataccgtga ctctcaagac
    14821 ttctccccgc cctcaagatc tcctccacct gatcctcatc aaccttgacc cgcccgagac
    14881 gcgctcctcc cagccccggc aactccgttc catctaccgc caaaatgacc cgccgcccat
    14941 ccagatctcc atccaggacg gcgcggcgtt ccttaacctg aactataact ataccggact
    15001 gccattgttt agtcaccttc gcactctcaa cccacggatt agtctcgacc tgccgtctga
    15061 gcatcgcggt gttgagcgtg aggaggctgg cgccgtcgga taaggactgc gagacctcgg
    15121 attccgggta catccgcgcg cccttgacct ggatgccggt aaccgggtag aggaggtagg
    15181 cgacgatgaa gaccacggct ccggtcaggc cggcgacggc tatcgagagg gagatgccgc
    15241 ggacgactct gcggagggtg ccgggcaagg ctacagtagt tccaacagct cttcgccgac
    15301 gcgggagatg tcgccagcgc ccatggtgag cacggtatcg ccccggttcg agatcgagcg
    15361 gaggacgttc gggatcgcgg cctgctccgg gatgtagtag acgtcgggat gctcgcgcgt
    15421 ctcgcagatg gcgtccacga tcagttttcc gcttactcca ggctgcggca tctcgcccgc
    15481 cccatacacg tccgtgatca ggacggcgtc cgctgcggtg aaggcctccc cgaactcgcg
    15541 gtgaagggta cgggtccggg agtagcgatg cggctgaaag acggcgatga cccggccctt
    15601 cgaggtggcg cgggcgacat cgagggttgc gagcagttcg gtcggatggt gcgcatagtc
    15661 gtctacgacc cgtatctcgc ctcgctctcc cttgaactga aaacgccgcc ggacgccgcc
    15721 gaagtccttc agcgtcctgg ccgcgtccag agcgtcgtgc ccgagtcgcc gggcaatgcc
    15781 gaccgcggcg agcgagttga ggatgttgtg ccgcccgaag acaccaagct cgattaggcc
    15841 acgctcttgc ccgtcctcca cgagcgtgta ggagttgggg ccgagtacca ccgcgcgaag
    15901 gtcaccggcg gtgatgccgt aagtcgttac ggagcacggc gcgctggacg cgagctggag
    15961 gcacgagggg tcctcggcgc aggtaacgag gtgtccgtct tcggggaggg cggcaacgaa
    16021 cttcgagaag gtatcgagca cgtcttcgag ggacgagtag aagtcggggt ggtcgaactc
    16081 gacgttggtg atgatggcgt tgtgcggacg caggtggagg agcgaacggt cgctctcgtc
    16141 ggcctcggcc actaccaggt ccgggctgcc ttctacgacg ttgctgccga tgtcgttcag
    16201 ctctcccccg accagagcgg tgggactctc gccgagagat cggaggacgt gggtcgtcat
    16261 gctggtggtg gtcgtcttgc cgtgagtgcc ggccacggcg atgctctgtc cgccctggag
    16321 gatcgtggcg agggcggcgg cgcggggtat gaccgggatg gacctgcgtc gggcttccag
    16381 aagttccagg ttcgtgggtg ggatggcagt cgagatgacg acctgctccg cgtccccgac
    16441 ctgatccgag gcgtgtccta tatagactcg catcccggtc tccttgagcc tgcgggtgta
    16501 ggcggactcc ttgaggtccg atcccgtaac agcgtgcccc cgtttctgga ggatctccgc
    16561 tatgccgctc atccccgctc cgccaatacc tatcatgtgt attctcatcg tcgtccatcc
    16621 tctctcgtag cggcgagcag ccttccggcc acctcatccg cggctccggg tgtcgcaagc
    16681 ccgcgcatgc gcgcggcgag gcgatcccgg cgttcgtcgt cttcaagcag gtcttcgatg
    16741 cgttcgcgca gcgtccgcgc gtttacctcc gagtcgagca tcaactccgc cgcgccgttc
    16801 tcggtaaagt aacgcgcgtt gtgtagctga tggtcgcccg tcgcgtacgg gaagggtatc
    16861 agaatggccg cccgtccggc cgccgccgta tcgaagagag aacccgcccc agaccggatc
    16921 acgaccacat ccgccgccgt cacgtagcgc cagatgtcgt ccacgtactc caggatgcga
    16981 tgccgggggt tgtccgtcga gagcctcggg aagtcccggc ggccggagat ctggacgacc
    17041 gtgtacggcg tctctccccg gaaggcctcc gcagccgcga ggtttatctt caacgctccc
    17101 ccactgccgc cgaagatcac gacgaccggc ggctccaatc ccaaatcatt caaagcctct
    17161 tcccgcgacg ccccgaagaa ctcccgccgc gtgggcatcc ccacccacac ggcgtccctg
    17221 agccgctcct tcgctgccgg gaaagtaacc aggacctccc tagtcagcct gctcgcgaag
    17281 cggttgaccc ggccgggcac cgagttctgc tcgtgcagga acgtcgggat accgagcgac
    17341 cgcgccgcta tcaccgccgg agcgctcgcg tatcccccca ctcccaatac cgcccccggc
    17401 tcgaagtttt gtattatacg tctacaactg tgtagggctt taaggaagag ggtggcggcg
    17461 cgggcgcggg cgagggggtt accggccagg cccttcaggg gtagggagtg gagtttgtag
    17521 ccggcctggg ggacgaggtc gcgttcgatg ccggactcgg cgccgacgaa ctccacggtg
    17581 gccccggaag cgcgcaggct atccgccacg cagagcgcgg gaatcgcgtg tccgcccgta
    17641 cccccgccgg ctatcaggac gcgcggcgct cttttttggt ttgatctctc ggactcgttc
    17701 actatcctcc gatatccggt acagaatacc tacggcagcg aagcagacta tcaggctgga
    17761 gccgccgtag ctgatgaacg ggagcgtcat accagcgagc ggcagcacgc ccatcgccgc
    17821 ccctatgtta aaggtagcct ggacggcgat cattgtggtg agccccgccg ccatacaccg
    17881 ggccatcacg gatggcgcgt ggaaggcgat gcggaggccc gccagcagca cgaagcacag
    17941 cgccgcgatg acggccatca tcccgaacag gccgaactcc tccccgatca gggcgaagat
    18001 catgtccgtc tggatctccg gcaccgtcgc cgcctgtccg ccggaaccgg cgcccgttcc
    18061 gaacaggctg cccgtcctta tagcgacgag ggactgaacg atctgatacc cggagtcgtc
    18121 ggagacggac cagggatcga ggaacgtcat gaaccgctgc cgccggtacg gctccagcca
    18181 catcacccca accagagcca cgaccgccgc gcctccggcc atcagcaggt cccgcgttcg
    18241 gacctctgac gcccacagca ccccggccac cccggcgaga agcaccaccg acgtgccgaa
    18301 gtcgggctca acgagtacca gcccgaagag cgcgccgacc gcagcgagcg gccggaatgg
    18361 gatcccggac ccggacggca ttcgcgccac cgcgcaactg agcgccacga ccgccgccag
    18421 cttggcgaac tccgctggct gcaggctgaa gatacccaga tccagccaac gacgcgcacc
    18481 gcccgcctcc acccctatcc ccggcaccag taccaggacc agaagagcga gcactgccac
    18541 gtacaaccac ggagcgaact tcctccagga cgtgtaccgt acccggctga ccagcaagaa
    18601 gacgatcacg ccgagcgtca cgtgccagag ccgcacgaac aggaagtgcg gcccatagtc
    18661 cctgtaagtc gccgagtaaa ccatcaccat accgagcagc gagagcccta gtgtcaccag
    18721 gaagaggttc gtcctcagcg acgccatcac ttcggggacc gttcacccat ctcccggacc
    18781 aggcggacca ggcgcgcaaa ctcctcaccg cgctccgagt acccggcgaa ctggtcgaag
    18841 ctggcgcatc ccggcgaaag caacacggcg tcgcccggcc tcgcgagcgt ctccgcccgt
    18901 gtcaccgcct cagctaggtc cgaaacgagg tggacgtctt cgacgccctt cgcccacaga
    18961 tattcataca ggcgcggtcc ggcctcgccc tggcacacga ccgcccggca tcggtttaga
    19021 taaggcaaga cctcaccgaa gtccgtgtcc ttctccgagc cgccgagaat gaggaccacg
    19081 gggcgctcga agctctccag cgccgcggcc acggctgcgg ggttggtggc cttggaatcg
    19141 tccacgtaga gcacgccacc gctctccgcg accacctgca tcctgtgcgg cttcagggag
    19201 tagcgttgca ggcccgctcg aacatccccg gaagccaccc ccagcttcct cgccgcggct
    19261 gcggcaaaca gagcgttctc gtagttgtgg gtcccggcga agcccagctc ggaggtcttc
    19321 gctaccaact caccttgttg aagaagctca tcctcaatga ccgctgacct accctcgccc
    19381 acaacgatag tcttcgccgc gagaccttcc gcagcgccgc gtccgacagg gtccgaggcg
    19441 cttattaggg cgaggtcatc tgagctttgt ccctcgaaga tcctcagctt atcttgcgcg
    19501 tactcctcga aagattcgtg ccagttgagg tgatccggac gggtgttgag cagcgccgcc
    19561 acctcgaacc tgccctcacc ccggtagtgc aactggaagg acgatacctc cagcgccagg
    19621 acgccggcct cgcgcacctc gtccaggcac gaagtcaatg cgcgccacga gttgccggcg
    19681 accgcgtgag ggaccccggc cgcatcgagt atgtggcgca acatgtccac gaccgtcgtc
    19741 ttaccgttcg tccccgtgac agcagccacg cgcacatccg ggcccagcat ctcaaggccc
    19801 aggctcacct ccgagatgat cgggattcca cgcgcttcgg cttcgcgtag tatcgggtcg
    19861 cgcggacgta tgccggggct tgccacgatg cgctccaccc cgtccagcac ggactcgtcg
    19921 gcaccaagcg agccctccac gccgagccgc tttagcgtct cctgcagttg ctccgagtct
    19981 ccagcgtcgg caacctggac cttctcgcct gcggagagga gagcctacgt cgcagaaacc
    20041 cccgactctc ccaggccgta tacgagagtc ctcacaatcc tggtctcaca ccgcgttgta
    20101 gaggtagaag tagtagagga cgaagcccag agccgcgaag gcggactgca cgatccagaa
    20161 ccgcacca
//
LOCUS       DASSMB010000162        10633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1306040, whole genome shotgun sequence.
ACCESSION   DASSMB010000162 DASSMB010000000
VERSION     DASSMB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10633
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306040"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..652
                     /locus_tag="VFJ72_09530"
     CDS             320..652
                     /locus_tag="VFJ72_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479740.1"
                     /translation="MRDRNLVFVPIRIGIAAAADGAWARMPDKVGVRKLYRVFRANQP
                     IFVGVGLAVLYWIAESALDVLMAGTGTFAGRLPPVEDANELWMRAIIVSLILGFGFYS
                     KAQRPADG"
     gene            657..2342
                     /locus_tag="VFJ72_09535"
     CDS             657..2342
                     /locus_tag="VFJ72_09535"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7479741.1"
                     /translation="MREGRNEPAGSGVERTAELARASEASRAEDSLRFMAEASAELSS
                     SLDYRDTLSGVARLAVPRLADWCAVDVITEDGSVERLAVEHEDRRKIALAIELQERYP
                     PEPESRSGLPNVLRTGRAECYPEISDEMLRSAATDEEHLGLLRELGFTSAMIVPMIAR
                     GRTLGAITLVSAESGRSYGEADLRLTEELARRAALAVDNARLYGEAQKEVAERRRAQE
                     ELRGSKDQLEIILRGVADGITAQEPSGRIIYANEAAARMVGYPSARALMEAPPGEVLR
                     KFEVLNEEGRPFPLERLPGRRALLGEDAEETLRFRVLGTGEERWAVVRATPVFDERGT
                     VRMAVNIFRDITERRRAEVALRELREAERRRIAHDLHDGVLQDLSYTAASMGLILLQT
                     EGTSLENEVQAAIDAVRRAARGLRDAVNDLRLEEETDRPFPELVESLVKRNRTMSRGG
                     YETSLEVEEGFLPAPLGETGTQLSRIIQEALTNARRHSGARNILVSLRIEGEDLVSEV
                     SDDGQGFMAAAAPGVGLKSMQERAQALGGTLEIESEPGKGTRVRIRVPLARKV"
     gene            2469..2645
                     /locus_tag="VFJ72_09540"
                     /pseudo
     CDS             2469..2645
                     /locus_tag="VFJ72_09540"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            2706..3020
                     /locus_tag="VFJ72_09545"
     CDS             2706..3020
                     /locus_tag="VFJ72_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase domain-containing
                     protein"
                     /protein_id="HET7479742.1"
                     /translation="MALADTDFFPAVFIASVGRERGFSEERISESFEKLRATLRKLEE
                     RLGDRGYLAGEFSLADVAYAGNLHRLRELEERGEVSLGEYPNVAAWVERIEQRESYKA
                     SG"
     gene            3543..3767
                     /locus_tag="VFJ72_09550"
     CDS             3543..3767
                     /locus_tag="VFJ72_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479743.1"
                     /translation="MRAMLCSCRGHLEASDEERLFEEVLAHLRRDHRAMDLEETRVRE
                     QVAGHSYGYEFLDVYAGAAEPDEEFGPEPY"
     gene            3822..4031
                     /locus_tag="VFJ72_09555"
     CDS             3822..4031
                     /locus_tag="VFJ72_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479744.1"
                     /translation="MWMIFRQTFGKLTMQHAVTVMPRGRCSPDAPDVDAHLQERGAAP
                     DRHHGPGVEEITAAEAPPIPYYDGS"
     gene            4089..4313
                     /locus_tag="VFJ72_09560"
     CDS             4089..4313
                     /locus_tag="VFJ72_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479745.1"
                     /translation="METASYGVVQTLEVSTGARERLSRPAAVGRCTSGPAPRPAQEVP
                     REDHRYTWLEDHLMRLEGKEARGEYYARLR"
     gene            complement(4530..6314)
                     /locus_tag="VFJ72_09565"
     CDS             complement(4530..6314)
                     /locus_tag="VFJ72_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479746.1"
                     /translation="MATPLRVLLIEDSENDAMLLLREFRRGGYEPFYERVDTPEAMEE
                     ALAGAEARGEPWEIVISDYFMPRFRAPDALALLRRLGYDMPFIVVSGKLGEETATEIM
                     RSGTQDYISKENMARLNPAVERELREVAVRRERRRAEKERDRFFALSLDLLCIAGMDG
                     YFKRVNPAFGKTLHYSEEELLSRPFIDFIHPEDRAATIAEVRKLAEGAQTTSFENRYR
                     CKDGSYVWLSWSGVPAVEEGLVYAVARDVTRHKRREQDLRESEERYRAVVEQAADGIL
                     LVEVESRSILEANAVYQELLGYAPEEILALTLYDVVPYSRESMDCYVERVLEQGQYTS
                     GERRHRRKDGTLVDVEVSANVVSYGGREALCIVVRDITERKWAEKTLREVREAERNRM
                     ARDLHDGVLQDLTYAVAAMQVTRIKAGGTSLESEIGRQIADLRKSVEGLREAVYDLRL
                     GEEEHRPLPQLVEALVNQNRPRARGLDISFHVEEGFPSTALGTPGVELLRIIQEALTN
                     ARRHSGAGNVQVILKVEDEELVVEVADDGRGFGPETSPGVGLSSMVERAAALGGNVRV
                     ESEVGEGTRVRARIPVPGVTSSTAAAPA"
     gene            complement(6451..7257)
                     /locus_tag="VFJ72_09570"
     CDS             complement(6451..7257)
                     /locus_tag="VFJ72_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7479747.1"
                     /translation="MCAGVDLEGVILDGSKRVGRGVPAYQRLSADLRAALKEGRFGTE
                     ESLPTEVDLAREYGVSRPTVRRAFQDLVAEGLVYRTPGKGTFPTALSRDSHYLGQIGT
                     IEDLTAFAGTEMEVMQPVELKADPEAASLLELPSRAVAAVVLRRLYEGVPFGLSRIYL
                     PPELGTRLVEADALPARGDTTVIGTLERLMSRIVAGANQVITAVPMPEDVAPVIDAEA
                     GEPSLRVQRLYIDDHEIPVELAVSYYNPRRYSHHLKMRRRTIPGRKPKSG"
     gene            7524..8033
                     /locus_tag="VFJ72_09575"
     CDS             7524..8033
                     /locus_tag="VFJ72_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET7479748.1"
                     /translation="MSIKEGWRGRFYEDFEVGDIYEHPLGRTVTTTDNIWFTLLTQNT
                     APIHFDHHYSAQTEFGKPLVDSTFTVALVTGQSVTDVSQNVMANLGWDEIRLPAPVFE
                     GDTIYSQSEVLEKRESKSRENVGIVTVKTTGYNQDGRVVITFKRTLMVYKKGHAPEIA
                     RVNLDTGSG"
     gene            8108..9256
                     /locus_tag="VFJ72_09580"
     CDS             8108..9256
                     /locus_tag="VFJ72_09580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7479749.1"
                     /translation="MATHEDLRQGVRKVCADFPDSYWRELDAKREYPEEFIQAMTESR
                     YLAPLIPEEYGGLGLSLTETTIILEEINRSGGNAQPAHAQVYVMGTLLRHGSEEQKRD
                     YLPRIASGELRLQAFGVTEPEAGTDTTQLTTSAVREGDHYVVNGRKIYISRVQHSDLM
                     VLLARTTPLDEVKRKSEGLSVFIFDLREAIGNGLTVNPRRVMINNETNELVFEDLKIP
                     ASSLIGEEGRGFRYILDGMNAERILIAAECIGNGKWFVDRATRRANERAVFGRPIGQN
                     QGIQFPIARAHISVEAADLMRFRAAEMFDKGEPCGAEANMALLLAADASFEAANAAMQ
                     TFGGYSYDAEYDVERKFRETRLFQVAPISTNLILSYVGEHVLGMPRSF"
     gene            9258..10454
                     /locus_tag="VFJ72_09585"
     CDS             9258..10454
                     /locus_tag="VFJ72_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET7479750.1"
                     /translation="MLPLEGITVISLEQAVAAPFATRQLADLGARVIKVERPGVGDFA
                     RGYDETVNGLASHFVWLNRSKESLTLNLKQEAAREVLEKLLSGADVFVHNLAPGAVER
                     LGFGARRLRTQYPRITVCGISGYGSNGPYTNKKAYDLLVQCEAGLVSITGTPETPSKV
                     GISVADIAAGMYAYTGVLTALFQRERTGEGSAFEVSMLEALGEWMGFPAYFAGYGGEE
                     PPRTGASHAAIAPYGPFECGDGDVVFLGIQNEREWALFCEVVLKRRELAEDPRFSSNS
                     RRVANREDLHSEIEAVLGTLPADDALLRLDEASIANARMRTVQGFLDHPQLAARERWR
                     EVGSPVGSLRALIPPATFDGDEPVMAPIPDVGEHTGSILAEIGYADSEIDALRRDGSI
                     SSETGG"
     gene            10451..>10633
                     /locus_tag="VFJ72_09590"
     CDS             10451..>10633
                     /locus_tag="VFJ72_09590"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7479751.1"
                     /translation="MSEHQTDDRSRVAARSLLAVPATNLKMVQKGLASSADGVFLDLE
                     DAVAPDRKEDARNDVIH"
BASE COUNT         2001 a         3299 c         3447 g         1886 t
ORIGIN      
        1 ctgcaggtct ctatatatga cctgtgggtg ttgggaccga cggggaagga cacggtaagt
       61 ttacagacgt gcatcaaccc gccggcatat gatgtgcggc tgatagttag gggagacctc
      121 accagcatag accccgcctg aagtgtatct acctgcttgc ccaaggacct cgtatacgga
      181 gcgtggcata aggaacgcgg cgcgctctcg gaggttcgtc ctgataaaag cgaggggctg
      241 ccggtcccgg tgcggggttg ttgccagcgt tacggtccaa taggattggg gttggatcct
      301 gaaagaaaaa gagcgatgct tgagagaccg gaacctggtt ttcgtgccga tccgaatagg
      361 gatagccgct gccgccgatg gagcgtgggc gaggatgcca gataaggttg gagtaaggaa
      421 actgtaccgc gtgttccggg cgaaccagcc gatattcgtg ggggtcgggc tcgcggttct
      481 ctactggatc gccgaatccg ccctcgatgt cttgatggcg gggaccggta cctttgctgg
      541 acggctgccg cccgtcgagg acgcgaacga gctctggatg agggccataa tcgtgagcct
      601 tatcttgggg ttcggtttct atagtaaggc tcagaggccg gcggacggat aagggcttgc
      661 gcgagggccg aaatgagccg gcgggctcag gagtagagcg caccgcggaa ctggcgcggg
      721 ccagcgaagc ctcgcgggcg gaggactctc tgcgtttcat ggccgaggcg agcgcggagc
      781 tctcttcctc gcttgactac cgggatacgc tctccggcgt tgcccgtctc gcggtgcccc
      841 gcctcgccga ctggtgcgcc gtggacgtta tcacggagga cggttcggtc gagaggctcg
      901 cggtagagca tgaggaccgg cgaaagatcg cgctagccat tgagttgcag gagcgttatc
      961 cgcccgaacc ggagtcccgc agcgggctgc ccaacgtgct gcgtaccggc cgcgcggagt
     1021 gctaccccga gatctccgat gagatgttga ggagcgccgc aacggacgag gagcatctcg
     1081 ggttgctgcg ggagctcggc ttcacctcgg ccatgatcgt gcccatgatc gcccgcggga
     1141 ggacgctcgg cgcgatcacg ctggtctcgg ctgagtcggg gcgtagttat ggggaagcgg
     1201 acttgcggct gacggaagag ttggcccggc gcgccgccct cgcggtggac aatgcgaggc
     1261 tgtacgggga ggcccagaag gaggtggccg agcggcggcg ggcgcaggaa gagcttcggg
     1321 gctcgaagga ccaactcgag atcatcctgc gaggagtcgc ggacggcata accgcccagg
     1381 agccctcggg acggatcatc tacgccaacg aggccgccgc gaggatggtc ggctaccctt
     1441 ccgcacgggc gctcatggag gctccgccgg gcgaagttct gcgcaagttc gaggtcctga
     1501 atgaggaggg taggccgttt ccgctggagc ggctgccggg acggagggct cttctgggag
     1561 aggacgcgga ggagaccctg cgcttccgcg tgctcggcac gggcgaggag cgatgggctg
     1621 tggtccgggc gacacccgtt ttcgacgagc gggggacggt ccgcatggcg gtcaatatct
     1681 tccgtgacat aaccgagcgc aggcgagccg aggtggcttt acgagagttg agggaggccg
     1741 agcgccgccg gatagcccat gacctgcacg acggagtcct gcaagacctg tcctacaccg
     1801 cggcatccat gggtctcatc ctgctccaga ccgagggaac gagtctggag aacgaggtac
     1861 aggcggccat agacgccgtc cgccgcgccg cccggggact gcgcgacgcg gtcaacgacc
     1921 tgcgtctgga ggaagagaca gatcgcccct tccccgagtt ggtcgagtca ctggtgaaga
     1981 ggaaccggac gatgtctcgg ggaggttacg agacgagcct ggaggtggag gagggatttc
     2041 tcccggcgcc gctcggtgag accggaacgc agttgtcgcg catcatccag gaggccctga
     2101 ccaacgcccg gcgccactcg ggcgctcgta atattcttgt gagcctgagg atcgaaggag
     2161 aggacctggt ttcggaggtc tccgacgatg ggcaaggctt catggcggcg gctgctccag
     2221 gggtaggact aaagagtatg caggagcgcg cgcaggctct gggcgggacg ctggagatag
     2281 agagcgagcc gggtaagggg acgcgggtgc ggatacgggt cccgctcgcg cggaaggtat
     2341 aagggaaccg ggatacgacc ttcgagacaa cggcctgcta ttatcggccg ggaatgcgcc
     2401 agctactcag ggagagacct tctatgaagc cgaggcttta taacacccag cagtgaccct
     2461 acgcccggcg gaccaggatg gtcctgcacg agaagggcat agacttcgag acctatgagg
     2521 tagatctcgg aaacaagagc gaggagttcc tggcggcgag cccgaccggc aaggtcccgg
     2581 tggtagtcgt cgacggagac tccctctacg agtccaacgt cgtcaaccag tacctcgatg
     2641 aggtgaacgg tgaaccgaag ttgatgcctc aggacccgaa agaacgggcc tacgctcgta
     2701 tctggatggc cctcgccgac accgacttct tcccggcggt cttcatagca agcgtgggac
     2761 gcgagagagg gttctccgag gagcggatct ccgagagttt cgagaagctc cgggcaacac
     2821 tccggaagct ggaggagcgc ctcggggatc gcggctacct cgccggcgag ttctccctgg
     2881 cagacgtggc ctacgcgggc aacctccacc gcctgcgcga actggaagag aggggagagg
     2941 tctctctcgg cgagtacccg aacgtcgcgg cctgggtcga gcggatcgag cagcgggaga
     3001 gctacaaggc gtccggatag tctccgcctc cgcgaggcgg agatccctct tcgcacccgg
     3061 gacatcaccg gctcgatgcg cggcgggaag ctgcggtatg cgatcagcgt attggccggc
     3121 atcttgcgct cccgccaaac tccctgggcc gatgcatcag ttcctggttc cggcggttcg
     3181 ctgcgccctg ttgggtgagt cgtccggatg gccgaactcg gcccggtaga aactggcccg
     3241 tgggccgatg atctccatga cccaaacgtc caaactgtac ccagagcgcc gatagagact
     3301 cgaggcgagg taccctgtgt cagaaccgcg cgcggataca tccagacttc tcggggagag
     3361 acgtactcca gcgtccatga tcaggcgcgc ctcccggctt cccgcaccag agtcccgcaa
     3421 tcgccttcgc gtaaacgata gggcaaaaga cctattcaga tcggggtttg ctcttaataa
     3481 catgcggtgc tcctggggag cagaggagga gagaatggac cgagaagaga caggaggacg
     3541 agatgcgagc catgctatgt agctgccggg ggcatttgga ggcctcggat gaagaaaggc
     3601 tcttcgagga agtcctggct catctgaggc gggaccaccg cgcgatggac ctggaggaaa
     3661 ctcgggtccg cgaacaggtc gccgggcact cctacggtta cgagttcctg gacgtctacg
     3721 cgggagccgc cgagccggac gaagagttcg gtcccgagcc ttattaggtc tcacgggcca
     3781 tcctcgcagc ggcactgaga cgcgggagag acggggtaac catgtggatg atattccgcc
     3841 agacattcgg gaagctcacc atgcaacatg cggtgactgt catgccgcgg ggccggtgct
     3901 ccccggacgc tcccgatgtg gatgcgcacc tacaggagcg aggagcggcg ccggaccggc
     3961 atcacggtcc cggagtggag gagatcaccg ccgccgaagc gccgcccatc ccttactacg
     4021 acggtagctg acaggagcga ctcctacgat tggtgggacc ggagccccgg agagaaagga
     4081 ataaactcat ggaaacggca tcttacggag tagtacagac cctggaggtc tccaccggcg
     4141 cccgggagcg cctctcgcgt cccgctgctg tgggacgttg caccagtggc cccgctccgc
     4201 gcccggcgca ggaagtccca cgggaagacc atcgttacac gtggctcgaa gatcacctga
     4261 tgcgcctcga aggcaaggag gcgcgcggag agtactacgc gcgtctgagg taggattacg
     4321 tgacgtaatc ctccgtgccc cactcgcgcc ccgggcctga ccatagtctc aagcgctgca
     4381 cggaagtccc tggcgacgag caagcgggcc atcctatccc gcggtccgtt cacatgggcg
     4441 cgttacgcaa gtcgcgttgc gcgagtcgcg ttgcgcatgg ctgctcagga ggctgagccg
     4501 tccgggttga ggctgggacg gtcggatctt caggcgggcg cggcggcggt gctactcgtt
     4561 acgccgggca cggggattct cgcccggacg cgggtgcctt ctcctacctc actctccacc
     4621 cgcacgttgc cgccgagcgc ggcggcacgt tccaccatgc tgctcagtcc tactcccgga
     4681 gaggtctccg gcccgaagcc gcgcccgtcg tcggccacct cgaccacgag ctcctcatcc
     4741 tccaccttca gtatgacctg cacgttcccg gctccggagt gacgccgcgc gttggtcagg
     4801 gcctcctgga taatgcgcag caactcgact cccggcgtgc cgagagcggt ggacgggaaa
     4861 ccttcctcga catggaagct tatgtccagt ccccgagccc tcggacggtt ctggttcacg
     4921 agggcttcga ccagttgagg caatggacgg tgctcttcct ctcccaggcg aaggtcgtat
     4981 acagcctcgc gaagcccctc gaccgacttg cgcaggtccg ctatctgccg gcctatctcc
     5041 gattcgaggc tagtgcctcc ggccttgatc cgggtgacct gcattgccgc gaccgcgtat
     5101 gtcaggtctt gcaggactcc gtcgtggagg tcgcgggcca tgcggttgcg ctcagcttcg
     5161 cgtacctcgc gaagagtttt ctcggcccat ttgcgctcgg ttatatctcg gaccacgatg
     5221 cacagcgcct cgcgtcctcc gtaggagacc acgttggcgc tcacctctac gtctactagc
     5281 gtgccgtcct tgcgccggtg gcgccgctcg ccgctggtgt attgtccctg ctccagcacg
     5341 cgctccacgt agcagtccat gctctcgcgg gagtacggca ccacgtcgta gagcgtcagg
     5401 gccaggatct cttccggggc atagccgaga agctcttggt acacggcgtt ggcctccagt
     5461 atgctcctgc tctcgacctc gaccagcagg atgccgtcgg cggcctgctc taccaccgcc
     5521 cggtatcgtt cctcgctctc ccgaaggtcc tgctccctgc gcttgtgcct ggtaacgtcc
     5581 ctggcgaccg cgtacaccag tccctcttcg acggcgggga cgccgctcca cgacagccac
     5641 acgtatgagc cgtccttgca gcggtagcgg ttctcgaacg aggtggtctg cgcgccctca
     5701 gctagcttcc taacctccgc gatggtggcc gcgcggtcct cggggtggat gaagtcgata
     5761 aagggcctgg agagtagttc ctcctcagag taatggagcg tcttcccgaa ggccgggttc
     5821 acgcgcttga aatatccgtc catgccggcg atgcacagca gatccagcga gagcgcaaag
     5881 aaacggtccc gctctttttc ggctcgacgg cgctcccggc gcaccgcgac ctcgcgcaac
     5941 tcccgctcta cggccgggtt cagacgggcc atgttctcct tggagatgta gtcctgcgtc
     6001 ccggagcgca tgatctccgt cgccgtctcc tcgcccagct tcccggagac cacgatgaaa
     6061 ggcatgtcgt atccgagcct ccgtagcagc gccagagcgt caggcgccct gaaacgcggc
     6121 atgaagtagt cggagatgac tatctcccag ggctcgcccc gcgcctccgc gccggccagc
     6181 gcctcttcca tcgcctccgg ggtatccacc cgctcgtaga acggctcgta tcccccgcgc
     6241 cggaactcgc gcagcaggag catcgcgtcg ttctcggagt cctcgatcag cagcacacgc
     6301 aacggtgttg ccacgactct tccttcctct acaagtactc tacaagtagc cgggccgaca
     6361 tgatttcgat ccacacttta ctattaatgc gctttctcac ggaagtttag catcgaacct
     6421 tcggccgcag ttaggatgcg gctttcgagg tcaaccgctc tttggttttc gtccgggtat
     6481 cgtccttcga cgcattttga ggtggtggga gtaccgtctg gggttgtagt agctcacggc
     6541 caactcgacc gggatctcgt gatcgtctat gtacaggcgc tgaacgcgca ggctgggctc
     6601 gccggcctca gcgtctatta ccggggctac gtcttccggc atgggcaccg ccgtgattac
     6661 ctggttggcc ccggcgacga tccgggacat cagacgttcg agggtcccga tcaccgtcgt
     6721 gtccccgcga gccggcaggg cgtcggcctc gactagccgc gtgcccaact cgggcggcag
     6781 atatatgcgc gagaggccga aaggcactcc ttcatacaga cgtctcagaa ccaccgccgc
     6841 caccgccctg gatggcaact ctagcaggct cgcggcctcc gggtcggcct ttaactcgac
     6901 cggctgcatg acctccatct cggtccccgc gaaggccgtc aggtcttcga tcgtgccgat
     6961 ctgtccgagg tagtggctgt ctctggagag agcggtcggg aaggttccct tgccgggcgt
     7021 cctgtacaca agtccctcgg ctaccaggtc ctggaaggcc ctccgtacgg tcggacggct
     7081 cacgccgtac tctctggcga gatctacctc ggtcggcagg ctctcctccg tgccgaagcg
     7141 tccctctttg agcgcggcgc gcaggtcggc cgagagcctc tgataggccg gtacgcctcg
     7201 acctactcgc ttggatccgt ccagaattac tccctcaaga tcaaccccgg cgcacactgt
     7261 ccgccacccg ccccggaaaa agcggctaac atatacaacc cgtattttag caaacgtaat
     7321 gactttacgc ttgacaactt ttatttgtca ctacataatg tttgtaaaca agaaatatgc
     7381 ttacatttat tgttggaaag ggaacgtaga taacgtggac tcgcggtcgg gggccgggca
     7441 actgactact ctggtggggt agtgcgggcg tgagatcggg gtcgaggata ccgcgaggat
     7501 accggagaag ggataggcgg ttcatgagca taaaagaggg ctggcggggg cgcttctacg
     7561 aagacttcga ggtaggggat atctacgagc acccgctggg gaggacggta acgacgacgg
     7621 acaacatctg gttcacgttg ctcacccaga acacggcgcc gattcacttc gatcatcact
     7681 actcggccca gaccgagttc ggcaagccgc tcgtggactc gacgttcacc gtggcgctcg
     7741 tcaccggcca gagcgtgacc gatgtctcgc agaacgtgat ggcgaatttg gggtgggatg
     7801 agatccggct gccggctccg gtgttcgagg gtgacacgat ttactcccag tccgaagtcc
     7861 tggagaagcg ggagtcgaag tccagggaga acgtcggcat cgtcaccgtc aagactaccg
     7921 gctacaacca ggacggcaga gtcgtcatca ccttcaagcg caccctgatg gtctacaaga
     7981 agggtcacgc gccggagatc gcgcgcgtca acctcgatac gggttccgga tgaggcaagt
     8041 ggcggcgagg gtgtttcgta ggatcggcgg ccaggccgct cacaggaggt aacagaccgt
     8101 gttggatatg gctacccatg aagatctcag gcagggtgtc aggaaggttt gcgccgactt
     8161 tccggatagc tactggcgcg agctggacgc aaagcgcgag taccctgaag aattcatcca
     8221 ggcgatgacc gagagtcgct acctggctcc gctcatcccg gaggaatacg gaggactcgg
     8281 cctgagcctc accgagacca cgatcatact cgaagagatc aaccgttccg gggggaacgc
     8341 ccagcccgcc cacgctcagg tgtacgttat gggcaccctg ctgaggcacg gctcggagga
     8401 acagaagaga gattacctgc ccaggatagc gagcggtgag ttgaggttgc aggctttcgg
     8461 cgttaccgag cccgaggccg ggactgacac cacccagctt acgacgagtg ccgttcggga
     8521 gggcgaccac tacgtcgtga atgggcgcaa gatctacatc tcccgcgttc agcattcgga
     8581 tctcatggtg cttctggccc gcaccacgcc gctcgacgag gtaaagcgca agtccgaggg
     8641 gctctccgtc ttcatcttcg acctgcgtga ggccataggc aacggcctca cggtaaaccc
     8701 gcgccgggtg atgatcaaca acgagaccaa cgagctcgtc ttcgaggacc tcaagatccc
     8761 ggcgagcagc ctgatcggcg aggagggcag gggtttccgt tacatcctcg acggcatgaa
     8821 cgccgagcgc atcctcatcg ccgccgagtg catcggcaac ggcaagtggt tcgtggaccg
     8881 cgcgacccgg cgcgccaacg aacgcgccgt cttcggccgc ccgataggac agaaccaggg
     8941 catacagttc cccatcgccc gggcgcacat aagtgtggag gctgcggatc tcatgcgctt
     9001 cagggccgcc gaaatgttcg ataagggaga gccgtgcgga gccgaggcca acatggcgct
     9061 gctgctggcc gcggacgcct ccttcgaggc cgccaatgcc gccatgcaga ccttcggcgg
     9121 ctactcctac gacgccgagt acgacgtgga gcgcaagttc cgcgagacgc ggctcttcca
     9181 ggttgcccca atctccacca acctcatcct ctcctacgtg ggcgaacacg tgctcgggat
     9241 gccgaggtcg ttctaggatg ctgccgctcg aaggaataac cgtcatctcg ctcgaacagg
     9301 ctgtggccgc ccccttcgcc acccgccagc tagccgacct cggcgcccga gtgatcaaag
     9361 tagagcgtcc gggagtcgga gacttcgctc gcggatacga cgaaacggta aatggtctgg
     9421 cgagccactt tgtctggctc aaccgctcca aggaatctct caccctgaac ctcaagcagg
     9481 aggcggcccg cgaggtactg gagaagttgc tctccggagc cgatgtcttc gtgcacaacc
     9541 tcgcccccgg cgccgtagag cggctcgggt tcggcgccag aaggttgcgg acgcagtacc
     9601 cgcgcattac ggtctgcggc atctccggtt atggctctaa cggaccatac acgaacaaga
     9661 aagcctacga cctgctcgtc cagtgcgagg cgggtctggt ctccatcacc ggcacgcccg
     9721 aaaccccttc gaaggtaggg atctccgtcg cggacatagc cgccggcatg tacgcctaca
     9781 ccggcgtgct caccgctctc ttccagcgcg aaaggaccgg cgaggggtct gccttcgagg
     9841 tctccatgct cgaagcccta ggcgagtgga tgggcttccc ggcttatttc gcaggctacg
     9901 gcggagagga gccgccccgg accggcgcga gccacgcggc catcgctccc tacggtccgt
     9961 tcgagtgcgg cgacggcgat gtcgtcttcc tggggataca gaatgagcga gagtgggcgc
    10021 tgttctgcga ggtggtgctg aaacgtcggg aactcgccga ggacccccgc ttctcatcca
    10081 actccaggcg cgttgcaaac cgtgaagacc tccacagtga gatagaggcc gtcctcggta
    10141 cgctcccggc ggacgacgcc ctgctccggc tggacgaggc aagcatcgcc aacgcgcgca
    10201 tgaggaccgt ccagggcttc ctggaccatc cacagctagc ggcccgcgag cgctggcgcg
    10261 aggtaggatc tccagtaggc tccttgcgtg ctctcatccc cccggccacg ttcgacgggg
    10321 acgagccggt aatggctcct ataccggacg taggcgaaca caccggaagc atactcgccg
    10381 agatcggcta cgctgactcg gagatcgatg cgctgcgccg ggacggttcg atctcttcgg
    10441 agacaggggg atgagcgaac accagacaga cgacaggtct cgcgtagccg ctcgctccct
    10501 cctggccgtc ccggccacaa acctcaagat ggtccagaag ggactggcct caagcgccga
    10561 cggcgtcttc ctcgacctcg aagacgccgt cgccccggac aggaaagagg atgcccgaaa
    10621 cgacgtcatc cac
//
LOCUS       DASSMB010000163        15529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_293427, whole genome shotgun sequence.
ACCESSION   DASSMB010000163 DASSMB010000000
VERSION     DASSMB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15529
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293427"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..2267)
                     /locus_tag="VFJ72_09595"
     CDS             complement(342..2267)
                     /locus_tag="VFJ72_09595"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HET7479752.1"
                     /translation="MSFTEKTAEAVAHPLEPLSAEEISAAAGILRKEQDLGETHRFVS
                     LALREPEKQRVLDFENGGEIEREAFAILLDRSDGKTYEAVVSLTEGAVKSWEDVPGVQ
                     PQVILDEFFECEQVVKESPEVQEALRKRGVTEFDGVMVDPWSAGHYGDEEEGRLLRAL
                     IWVKIGGPDDNGYAHPVENLIVYVDMNRGRVVRIEDHGVIPVPQTPANYVPEEVGQMR
                     TDLKPVEITQPEGVSFRVNGHEVRWQKWRFRVGFTPREGLVLHTVGYEDDGRLRPILY
                     RASLSEMVVPYGDPAPTHSRKNAFDAGEYNIGQLANSLELGCDCLGEIRYFDATLADS
                     SGEPYAIKNAVCMHEEDYGLLWKHTDFRTERTEVRRSRRLVISFVATVGNYEYGFFWY
                     LYQDGNIEFEAKLTGIMSTGAVEPGEKPKYGQLLDKDGLYAPIHQHFFSMRLDMDVDG
                     RENSVYEVNTESAPPGPENPLGNAFFTRSTLLGRESEAQRVVDPFSARYWKVVNPSVE
                     NAAGEPVGYKLIPHGNVLPFAQPDSSVLRRAGFMTKHLWVTPYEPSEMHAAGDYPNQH
                     QGGEGLARWTAADRQTEDTDVVVWYTFGSHHAARLEDWPVMPVQYAGFMLQPVGFFDQ
                     SPALDVPPSSHNGHCDH"
     gene            complement(2320..3891)
                     /locus_tag="VFJ72_09600"
     CDS             complement(2320..3891)
                     /locus_tag="VFJ72_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7479753.1"
                     /translation="MERSEAQAGGAIAREDGGVQEFQRLKSNSIGLLGVLFMVVATAA
                     PISAMTANVPIGVGFGSEIGTPATYLLVTIILTVFTVGYVTMARYITATGAFYAYISH
                     GLGTVMGMASGILAVMAYVVFEAAVAGLFAAFAQTTIGDQFGLNVPWPILAVLLLALN
                     AFFGHRAIDLTAKVLGFFLATEIAILALMAGSVFLAGGGPDGIPLSPLNPANAFLGAA
                     PAIGFVFAFWSWVGFESTAIYGEESREPKQMIPRATYIAVIGLGLFYTWVSWAAIAGN
                     GLAESVKLAQSANPFALLFDPTQQFVGGWAVVLYQWLLITGSFAVGLAFHNCAARYLY
                     ALGREGLISRGLGTIHATHGSPAVASLTQTVIATVIVGLFWAFGQDPYLGLFSLMGVL
                     GTVAILCVQGICSFAVIAYFRRNHPEARHWWRTLVAPVLGGLAIFGVVALLVQNLATA
                     LGPAAETPFGRSIPYIVIGVFLIGVALALYLRARSPERYALIGKVIYEDATERPDIDL
                     DGDGRGDHAVRPGRS"
     gene            4089..4922
                     /locus_tag="VFJ72_09605"
     CDS             4089..4922
                     /locus_tag="VFJ72_09605"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7479754.1"
                     /translation="MTKDPLYVRVEKRIEDLLVRGRYRVGERIPPESELVKSLGVSRV
                     TVRAGLARLVERGLLERRQGSGTFLARFPEEGRPRAGGIGFKLGSAQADLGSLETYTV
                     LAERMGLELSSRDLRIERIGADSEQAEALEVPEGSGLVRVSRVLLADGDPAAWMVDVV
                     PEGIISVDRVRERFRPDAMLLDVLVSEGVPVGFSEVLIEAEMVLPEDWTAEALELESP
                     SAALSLTETMYLETAHPADGRPVQWSRNLFLPGRLKLHIVRELAEQRDPSLVFRLPSN
                     R"
     gene            complement(5095..6435)
                     /locus_tag="VFJ72_09610"
     CDS             complement(5095..6435)
                     /locus_tag="VFJ72_09610"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET7479755.1"
                     /translation="MDLETLRSETEAGRVDTVLVAAVDMQGRLQGKRLTAKHFLDEVV
                     EHDAGGCSYLLAVDVEMNTVGGYEMTSWAQGYGDFAYKPDLETLRYVPWHECTAMVMC
                     DLELKDGTPVLASPRHLLKSQLGRLAERGLEALVGTELEFLVFRDTYQEAWRKGYRDL
                     EPATRYNVNYSLLDTARLEPLLRRIRNSMVGAGLPVETAVGECNSGQHEINLRFGTAL
                     AVADGHAIYKNGAKEIAAQEGYSISFMPKFDEDAGNSCHIHLSLRDREGDAAFAGPQG
                     LSKRFQGFLAGQLACVRELMLFFAPNINSYKRYSDYSAAPTRVAWGRDNRTCAFRIVG
                     QGSSLRIENRVPGGDANPYLAIAAMLAAGIHGMDRNLALEEELAGDAYAATGKPSFPK
                     SLQEARELFANGEVVREAFGEEVVDHYLNMARVEIEAFEAAVTDWERYRNFERT"
     gene            complement(6637..6831)
                     /locus_tag="VFJ72_09615"
     CDS             complement(6637..6831)
                     /locus_tag="VFJ72_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7479756.1"
                     /translation="MAGVEAARAHGRKGGSQPVMDEKKLALASKMLKNREIPVGEVCE
                     PVGMSRSTLYRHLRPDGSAR"
     gene            complement(6808..6909)
                     /locus_tag="VFJ72_09620"
                     /pseudo
     CDS             complement(6808..6909)
                     /locus_tag="VFJ72_09620"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(6943..7692)
                     /locus_tag="VFJ72_09625"
     CDS             complement(6943..7692)
                     /locus_tag="VFJ72_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET7479757.1"
                     /translation="MAAGLTGFDGTTQTAREISGGLRRLVRAGRVVLVNDAVTSYLGA
                     IGFEPGVVVAAGTGVIALAGDTEGNFVRGNGWGYVLGDDGGGYYVGRRGLAAALRRHD
                     GRGGSEDLLRRAEAVYGPPEAIKGRVYAADNPPGVVAGFAPEVAEAARAGDPEAAEIW
                     ADAAREVALTATACLGRVFAPDAPVTVSWTGSLFDARDLMLEPFRRHVEESWPAARLF
                     PPTGDAIEGAGLLCAPRASSMFAPLIHVSGH"
     gene            complement(7891..8412)
                     /locus_tag="VFJ72_09630"
     CDS             complement(7891..8412)
                     /locus_tag="VFJ72_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7479758.1"
                     /translation="MPSAEARKLPEGAREILECRWEGTARYRYADHMVVTSRGFNLPT
                     AQEAALKLMETSYVPAQPFSEADLRHGPLAMVTEDFPVFVIVPPGRARASVASLVDTL
                     NERGAELVVMDEDGGTLEKASASFRVPASVPEELSPILYAIPPQLFAHDLSASRGLDP
                     DSPRGLSKVTESW"
     gene            complement(8402..8623)
                     /locus_tag="VFJ72_09635"
                     /pseudo
     CDS             complement(8402..8623)
                     /locus_tag="VFJ72_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013161536.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8887..9162
                     /locus_tag="VFJ72_09640"
     CDS             8887..9162
                     /locus_tag="VFJ72_09640"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HET7479759.1"
                     /translation="MEFGGRLKEAGLLLSMGSVADAYDNALAESFVATLKSELLHRHS
                     WPTKERLRVAIFEYVEGFYDRKRRHSALGYLSPAEYEEGRMSEVTVA"
     gene            9366..9743
                     /locus_tag="VFJ72_09645"
     CDS             9366..9743
                     /locus_tag="VFJ72_09645"
                     /inference="COORDINATES: protein motif:HMM:NF015162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET7479760.1"
                     /translation="MGEAISIKGATDAELFEAYVEDFLASTLKVGQVVVLDRLGAHRT
                     SRVRELVEERGAQLLFLPSYSPDYNPIEEAFSKIKNIVRKAGARTREALDEAISEALS
                     SVTLEDVAGWFSHCGYQPRGQCL"
     gene            complement(9856..11037)
                     /locus_tag="VFJ72_09650"
     CDS             complement(9856..11037)
                     /locus_tag="VFJ72_09650"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7479761.1"
                     /translation="MKLAKRMGRLGTETAFSVLAKAKALEARGREVIHLEIGEPDFPT
                     AENIVEAACRALRDGHTHYTPTAGIPELREAIAADVSRSRGIAVEPDQVVVTPGGKPI
                     MFFAILALVERGDEVLLPSPAFPIYESMINFAGGTPVFLPLRQENGFRLDPDEFRAGL
                     SERTKLVILNSPANPTGGVLTAEDIAGLAEMLREHPDVFVLSDEIYSRLVYSGGFASI
                     ASEPGIGPDERTIILDGFSKTYAMTGWRLGYGVMPRPLAEEIATLQVNSNSCTNAATQ
                     YAGLEALTGPQESVKEMLKEFRARRDLIVAGLNDLPGVECATPQGAFYAFPRITDTGR
                     DADALADQFLEESGVACLSGTGFGPYGEGHLRFSYANSRQNIIRGLERIGDLLARSTL
                     Q"
     gene            11167..12036
                     /locus_tag="VFJ72_09655"
     CDS             11167..12036
                     /locus_tag="VFJ72_09655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7479762.1"
                     /translation="MGARNNGLKNDSRKNGLSFELGAFDLDGTVLRRDLVISEKTVAA
                     LGRLRERGMRLVVATGRRYESAREHAGRLGFGDEDPVICYGGSMVRRMNGETLLHRTL
                     PTDLSIEALEWASERGLHHRILMDDTIVVSQDPPTSLERLSNPGEPETFSVESPARWL
                     RETGAEPTKLVIVDTPDGVECWLREAQDAFAGRLFVTRSLPHYVEISNLEGTKSNALA
                     FLCDKWSVEPARTLAFGDADNDIDMLRFAGHGVAVGGMTEEVREVADAVAPPVNEDGV
                     ARYIEKLLGGIDA"
     gene            12029..12844
                     /gene="mutM"
                     /locus_tag="VFJ72_09660"
     CDS             12029..12844
                     /gene="mutM"
                     /locus_tag="VFJ72_09660"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET7479763.1"
                     /translation="MPELPEVTVISEDVAALAKGRQVLRAAVFRPDVTNVEPEEFTRR
                     LVGKTLRGTGRRGKIIVLDFGDVIGIVHLVISGRVLRLDEWTQPDRMNTAVLEFGGGL
                     VLAFTRLWLGYFDLYEPGEEEKHPLIARLGPDPFSEAFTPEYLAEMFQRRASVKGLLL
                     DQSVVAGLGNIYVDEVLFAAGVHPSRKAGTLSVEEIQRIHAATRDILGRAIELRGTTF
                     DSYHDAFGETGKYQHELKVFTRAGQPCPACGTQIEKSRVAGRGTHVCPSCQAP"
     gene            12914..14086
                     /locus_tag="VFJ72_09665"
     CDS             12914..14086
                     /locus_tag="VFJ72_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479764.1"
                     /translation="MSAPVDSFLERHPNAARNIARYGWLALLVLAALLLAGGTVLAGG
                     RSQQIVYATSDGSIVSLEPVSGSSTILYKGGAGHYATMPGRTGSRSISFTVLRDDGGS
                     VRGNLYGADIVRETRALLQDARPGEVFAYPDYAADREWVMANRFTRDTPPNVEVFTAS
                     AAASRLLEPDTPGASALAGPSWTAERSIYAWRVGQEKTSLTAYNFFERRQAAVYETKD
                     VVGPASYYFDGNAFLFAERPRGAGLEDSRIKVLVGTGTAKISGVGELGLYDPSSPATV
                     LGGKISVIWTDGEKTGIGMIDPRGWTFSKTGIEVAAGSRDPRLSNDGLYVATTDSAGK
                     EITVRRLDDGGVVRRIRDAQPPETAIDRMREAGIKVPEETGWLAPPDYGWRSLEDT"
     gene            14073..>15529
                     /locus_tag="VFJ72_09670"
     CDS             14073..>15529
                     /locus_tag="VFJ72_09670"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HET7479765.1"
                     /translation="MKTRSPQPPKVQKAAGARRRLGPRELVICCAVVYAVLISGLSMY
                     KHETYASSRFDLGNMDQAVWNSAHGRILEATDEYGEITSRLKNHTDFLLLAFVPLYWI
                     SPSPHWLLVTQAVVVGIGAVPLYWLARRFLGYELPAALLAVAYLFNPGLQSANLFDFH
                     AQMMAGTFLLFAFHYLLERRLLPFLAFATLAALTKEGIVLIVGMMGLYVLLVERRPRW
                     GIPVFLASAGYFLLTMLVIIPFFNTGGASELVEGRYAAFGGSLGGVVRTTLAEPIFTI
                     SYVLSQQKALYLTYLTGMTWFFGLFSPTTLVLPLPDLAVNLLSDRPQMVNIRYHYSAT
                     IMPFTYVAAAAGLANVLYFFRRAANWRRPFSWLGRLARREVSSERTAVVLTAGMLLFA
                     AQMDYDYGPLPVFHSPGNFASVIKPAPEEHLRALDEAVAMIPDGAKVSASNQIGPHLS
                     YRRYLYLFPDLGDARYVILDETEPGYDTYINPVLNL"
BASE COUNT         2596 a         5164 c         5036 g         2733 t
ORIGIN      
        1 gtcacggtct gcggcccttc ctccagcgcg cgccgtatca attcgctgaa cctctgcttc
       61 gctttctgcc cctgccaagt catcccgcca actcccattc tgtccactct gtccaggact
      121 atatcgaatt ggtgggtcgt agcgccgcgg ccgcctcggt ttccgggcgc gagaaacaac
      181 tccccgaagg cgcgattcga cttccctcgt atgctcgacc cgtccaggga tctcggccga
      241 cgccagacac atccaggccg cggctatcag gcatacccat ggagacttga gctgctcgcg
      301 gaacggcacc cttcgcccct gcccctagta ggtcgctccg ctcagtggtc gcagtgaccg
      361 ttgtggctgg acgggggcac gtccagggcc gggctctggt cgaagaaccc gacgggctgg
      421 agcatgaagc ccgcgtactg caccggcatc accggccagt cctccagacg tgcggcgtgg
      481 tgcgagccga aggtgtacca gaccaccacg tcggtgtcct ccgtttgccg gtcggccgct
      541 gtccaccggg ccaagccctc gccgccctga tgctggttcg ggtagtcgcc ggcggcgtgc
      601 atctcggacg gctcgtaagg ggtcacccag aggtgcttgg tcatgaaccc cgctcgcctg
      661 aggacgctgg agtcgggctg ggcgaagggc agcacgttgc cgtgggggat cagcttgtag
      721 cctaccggct cgcccgccgc gttctccacg gacgggttga ccaccttcca gtaccgggcg
      781 ctgaaggggt ccacgacccg ctgggcctcc gactcccggc cgagcagggt ggatctcgtg
      841 aagaaggcgt tgcctagcgg gttctccggg ccggggggag cggactcggt gttgacctcg
      901 tagaccgagt tctcccgccc gtcgacatcc atgtcgaggc gcatgctgaa gaagtgctgg
      961 tggatcgggg cgtagagccc gtccttatcg aggagctggc cgtacttggg cttctctccg
     1021 ggctccacgg ctccggtgga catgatcccg gtgagcttcg cctcgaactc gatgttgccg
     1081 tcctggtaga ggtaccagaa gaaaccgtac tcgtagttgc cgacggtggc gacgaaggag
     1141 ataacgagcc ggcggctcct gcgcacctca gtcctctcgg tgcggaagtc agtgtgcttc
     1201 cacaagagac cgtagtcctc ctcgtgcatg cagacggcgt tctttatggc gtacggttcg
     1261 ccgctgctgt ccgccagggt ggcgtcgaag tagcggatct cgcccaggca gtcgcacccg
     1321 agctctagcg agttggcgag ttgtccgatg ttgtactcgc cggcgtcgaa ggcgttcttg
     1381 cgggagtggg tgggcgcggg gtcgccgtac ggcaccacca tctccgagag cgaggcgcgg
     1441 tagagtatgg ggcgcagcct cccatcgtcc tcgtacccga ccgtgtggag cacgagcccc
     1501 tcgcgggggg tgaagcctac gcggaagcgc cacttctgcc agcgcacctc gtgtccgttc
     1561 acccggaagc tgacaccttc gggttgcgtg atctccacgg gcttgaggtc ggtccgcatc
     1621 tgcccgacct cttcggggac gtagttggct ggggtctgcg ggacgggtat cacgccgtgg
     1681 tcctctatgc ggaccacccg gcccctgttc atgtccacgt agacgatcag gttctcgacg
     1741 ggatgtgcgt atccgttgtc atcggggcca ccgatcttca cccagatcaa agcccgcagc
     1801 aggcgcccct cttcctcatc accgtagtgt cccgccgacc aagggtccac catcaccccg
     1861 tcgaactcgg tgacgccgcg cttcctgaga gcctcctgga cctcagggct ctcctttacg
     1921 acctgctcgc actcgaagaa ctcgtcgagt atgacctgcg gctggacgcc ggggacgtcc
     1981 tcccacgact tgaccgcacc ctcggtgagc gacaccaccg cctcgtaggt ttttccatcc
     2041 gagcgatcca gcaggatggc gaacgcctcg cgctcgatct ccccgccgtt ctcgaagtcg
     2101 aggacgcgct gcttctccgg ctcgcgcagg gccagcgaga cgaacctgtg tgtctctccg
     2161 aggtcttgct ccttgcgcag tatgcccgcg gcggcggaga tttcctccgc gctcagcggc
     2221 tcgagggggt gggcgacggc ctcggccgtc ttttcggtaa acgacatctg tgttcctcct
     2281 tctagcctgt cttgggtctt ggcgaaagga ccggaagacc taactcctcc cgggcctgac
     2341 cgcgtggtcg ccgcggccgt cgccgtcgag gtctatgtcc ggccgctcgg tcgcgtcctc
     2401 gtagatgacc ttgccgatga gcgcgtaccg ctcagggctc ctcgccctca ggtagagcgc
     2461 gagcgcgacc ccgatcagga agaccccgat cacgatgtaa gggatggacc tcccgaaggg
     2521 ggtctcggcg gcgggtccca gagccgtggc gaggttttga accagcaggg caacgacccc
     2581 gaatatggca agcccgccga ggaccggcgc gacgagggtc cgccaccagt ggcgcgcctc
     2641 ggggtggttg cgacggaagt acgcgatcac cgcgaacgag cagatgccct ggacgcagag
     2701 gatggcaacc gtcccgagca cccccatcaa ggagaagagg ccgaggtacg gatcctgccc
     2761 gaacgcccag aagagcccca cgatcacggt ggctattacc gtctgggtca gagacgcgac
     2821 cgcgggtgag ccatgagtgg cgtggatcgt gccgaggccg cggcttatga ggccctcgcg
     2881 gccgagcgcg tacaggtaac gcgcagcgca gttgtgaaag gccagcccga cggcgaacga
     2941 acccgtgatc agcagccact ggtagagcac gaccgcccac ccgccaacaa actgctgggt
     3001 gggatcgaac agcagggcga aggggttggc gctctgggcg agcttgaccg attcggccag
     3061 gccgttcccg gcgatggctg cccacgacac ccaggtgtag aagaggccga gccctataac
     3121 ggcgatgtag gtcgcccgcg ggatcatctg cttcggctcc cgcgactcct cgccgtatat
     3181 cgccgtggac tcgaagccca cccacgacca gaacgcgaac acgaacccga tggcgggcgc
     3241 cgctcccagg aaggcgttag ccgggtttag gggagacaac ggtataccgt ccggccctcc
     3301 gccggccagg aacacactcc ccgccatgag ggcgaggatc gcgatctcgg tcgccaggaa
     3361 gaacccgagg accttcgccg tcagatcgat ggcccggtgg ccgaagaacg cattcagggc
     3421 gagcagcaac accgccaata tcggccaggg tacgttcagc ccgaactggt ccccgatggt
     3481 ggtctgcgcg aaggccgcga agagcccggc aaccgccgcc tcgaacacaa cgtaggccat
     3541 caccgccagt attcccgatg ccatgcccat cacggtgccg agaccatgcg agatgtacgc
     3601 atagaaggcc cccgtcgccg tgatgtagcg cgccatcgtc acgtacccga ccgtgaagac
     3661 cgtgaggatg atcgtcacca gcaggtaggt cgccggcgtg ccgatctcac tcccgaaacc
     3721 cacgccgatc ggaacgttcg ccgtcatcgc cgagatgggc gcggccgtgg cgacgaccat
     3781 gaacagcacc cccagaagac cgatggagtt ggacttcagc cgctggaact cctgaacccc
     3841 gccgtcctcg cgtgcaatcg ccccacccgc ctgcgcttcg cttctctcca taaactactc
     3901 cttacctctc caccgctttc gttgcatatc aacgacgcga ataggactat aactaatacc
     3961 aatttgtatt gcaatacaaa ttggtagcaa agcataatga atttgcatcg ggcgtgctaa
     4021 aatgcaggaa aagctcggcg tagaggaggt atatgatagc cgcaggacag caggagggat
     4081 tcgggccctt gaccaaggat cccctctacg taagggttga gaagcgcata gaagatctgt
     4141 tggttcgggg ccgttacagg gtgggcgagc gcataccgcc cgagtcggag ttggtcaaga
     4201 gcttgggggt ttcccgggtg acggtccggg cgggcctggc gcggctggtc gagcggggct
     4261 tgctggagcg gcgtcagggt agcggtactt tcctggcccg attccctgag gagggccggc
     4321 cgcgggccgg gggaataggc ttcaagctag gcagcgcgca ggccgacctt ggcagcctgg
     4381 agacctacac ggtgctggcg gagcggatgg ggctcgagtt gagcagccgg gatctccgca
     4441 tcgagaggat cggggccgat tcggagcagg cagaggccct cgaagtaccg gaaggaagcg
     4501 gcctggtgag ggtgtcgcgg gtgctgctcg cggacggcga tcctgctgcc tggatggttg
     4561 acgtggtacc ggagggcatc atctccgtcg acagggtcag ggagcggttc cggccggacg
     4621 cgatgttgct cgatgttctc gtttcggaag gcgttcccgt cggcttcagc gaggtgctca
     4681 tagaggcgga gatggtcctg ccggaagact ggacagccga ggcgctggag ctcgaatcgc
     4741 cctccgccgc gctttctcta accgaaacga tgtacctgga gaccgcccat cccgccgacg
     4801 gcagaccggt acaatggtcg cgcaacctct tcttgcccgg acggctcaag ctgcacatcg
     4861 tccgggagtt ggccgagcag cgggatccct cgctggtctt ccgccttccg agcaatcggt
     4921 aacagaggcc ataaccactc cgtatgattg gcctgattca ggcgaaaggt caaccccatc
     4981 gcgctctctg tcggaggaga tctcgtacgg gcgtcgagca gggccgaccg gcccgagcag
     5041 gatggtcgcc agcctgcggt ccgttcgtcc cgtagatctc ggcacccaac cgcttcacgt
     5101 gcgctcgaag ttccggtagc gctcccagtc ggtgacggcg gcctcgaacg cctcgatctc
     5161 gaccctcgcc atgttcaggt aatggtcgac gacttcctct ccgaatgcct cgcggacaac
     5221 ttcgccgttg gcgaagagct cgcgggcctc ctgtaacgac ttcgggaagg agggtttgcc
     5281 ggttgcggcg taggcatcgc cggcgagctc ttcctccagt gccaggtttc tgtccatgcc
     5341 gtgtatgcca gcggccagca tggccgcgat ggcgaggtac gggttggcgt ccccgccggg
     5401 gacgcggttc tcgatcctca acgaactgcc ctgacctacg atgcggaagg cgcaggtgcg
     5461 gttgtccctg ccccaggcca ccctcgtggg cgccgcggag tagtccgagt agcgcttata
     5521 cgagtttatg ttaggtgcga agaacagcat aagctcgcgc acgcaggcca gttgcccggc
     5581 caggaagccc tggaacctct ttgagagccc ttgaggtccg gcaaatgcgg cgtcgccctc
     5641 ccggtcgcgc aagctcaggt ggatgtggca ggagttcccg gcgtcctcgt cgaacttggg
     5701 cataaaggag atcgagtagc cttcctgggc ggcgatctcc ttggccccgt tcttgtagat
     5761 cgcgtgcccg tcggcgacgg cgagggccgt accgaacctg aggttgatct cgtgctgccc
     5821 ggagttgcac tcgcccacgg ccgtctccac cggcagacct gcgccgacca tcgagtttcg
     5881 gatgcgcctc aggagcggct ccaggcgtgc ggtgtccagg agcgagtagt tcacgttgta
     5941 gcgggtggcc ggttccaggt ccctgtagcc cttccgccac gcctcctggt aggtgtcccg
     6001 gaagactagg aactcgagct ccgttccgac gagcgcctcg agcccgcgct ccgcgagccg
     6061 ccctagctga ctcttcagta aatggcgcgg cgaggcgaga accggcgtgc cgtccttcaa
     6121 ttccaggtca cacatcacca tcgccgtgca ctcgtgccag gggacgtatc tcaaagtctc
     6181 cagatccggc ttgtacgcga aatccccgta cccctgtgcc caggaggtca tctcgtagcc
     6241 cccgacggtg ttcatctcga cgtccaccgc cagcaggtag ctgcatcctc cggcgtcgtg
     6301 ctcgaccact tcgtccagga agtgcttcgc ggtcaggcgt ttcccctgta acctcccctg
     6361 catgtccacc gcagcgacga gcacggtgtc cacacgaccg gcttcggtct ccgagcgcaa
     6421 agtttcgagg tccaaatttc ccgatctttc cttaaatccc atacacgaac ctccctcacc
     6481 cgaatcttaa aggatttcaa gcatccgcga aggtaagagg atgcatcgac tgtaattggc
     6541 acaggtagcg tcacagtact caagcgggat ctccgtacga tacgacttac gccgtaaatg
     6601 cagggaaccg tgagaggcgt gttttgcgtg cccggcttac cttgccgacc cgtcgggcct
     6661 gaggtggcgg tagagcgtcg agcgcgacat acccaccggc tcgcacacct cccccacggg
     6721 tatctcgcgg ttctttagca tcttggaggc caaggcgagc ttcttctcgt ccattaccgg
     6781 ctggctgccg cccttcctgc cgtgggctct cgccgcctcg acgcccgcca tcgtcctctc
     6841 gcggatgatg tcgcgctcga actcggcgac cgccccgaag acgtgggaca ccagcttgcc
     6901 gccaggagtc gtggtgtccg ggcgctgccc cggaagcggc gttcagtgtc ccgagacgtg
     6961 gatcaggggc gcgaacatcg aggaggcgcg cggcgcgcag agcaaccccg cgccttcgat
     7021 ggcgtcgccc gtcgggggga agagccgggc ggccggccac gactcctcca cgtggcgtcg
     7081 gaagggttcg agcatgaggt cgcgggcgtc gaagaggctc ccggtccagg agaccgtgac
     7141 gggagcgtcc ggggcgaaga cccggcccag gcaggccgtc gcggtcagcg ccacctcccg
     7201 ggcggcgtcg gcccagatct ccgcggcttc cgggtcgcct gcgcgggcgg cttcggccac
     7261 ctcgggcgcg aagccggcca ccacgccagg agggttgtcc gccgcgtaga ccctgccctt
     7321 tatggcctcc gggggcccgt agacggcttc cgcccgccgc aggaggtcct cggagcctcc
     7381 cctgccgtcg tggcggcgga gggcggcggc cagtccccgc ctgccgacgt agtagcctcc
     7441 cccgtcgtcg cccaggacgt agccccagcc gttgccgcgg acgaagttcc cctctgtgtc
     7501 tccggcgagg gctatgacgc cggtcccggc ggcgacgacc acacccggct cgaaaccgat
     7561 tgcgcccagg taggaggtca cggcgtcgtt gacgagcacc acgcgtcctg cccggaccag
     7621 cctccggagg ccgccggaga tctcgcgggc cgtttgggtc gtgccgtcga atccggtgag
     7681 gcccgccgcc accgcctcca cttaccccgg gcgcgcggcc ccaggctggc gagggcgcct
     7741 tgcatggctc ggaggatgcc ggcggggcga tcccggtcga ggcggctgag tcccgccgcg
     7801 cggcccgtca ggacgcgctc accgtcaacc gcgtagacca cgcggcaccc gctctgtccc
     7861 ccgtcgaata agtaggtggc ggctcatcgc ctaccagctc tcggtgacct tgctcaggcc
     7921 cctgggggag tcggggtcga ggcccctgga ggcggagagg tcgtgggcga agagctgggg
     7981 cgggatggcg tacaggatgg gcgagagctc ctccggcact gatgccggaa cgcggaagct
     8041 cgccgaggcc ttctccaggg ttcccccgtc ctcgtccatc accacgagct cagcgccgcg
     8101 ttcgttcagg gtgtcgacga ggctcgccac gctggcgcgg gccctgcccg gaggtactat
     8161 aacgaagacc gggaagtcct ccgtcaccat tgccagggga ccgtgccgga ggtccgcctc
     8221 ggagaagggc tgggccggca cgtaggaggt ctccatgagc ttgagtgcgg cctcctgggc
     8281 ggtcggcagg ttgaaccccc gcgaggtgac taccatgtgg tcggcgtagc ggtagcgggc
     8341 ggttccttcc caacggcatt ctaggatctc ccgggcgcct tccggcagct tcctggcctc
     8401 ggcgctcggc atgccactgc gctgtcgtgg cccgatactc caacccgccg ccacgggtgg
     8461 tggcgttacg gcgcagctct tgggagatcg ttaccgggtc gcggccgatc cggcgggcaa
     8521 tgtctcgaac gccacagttc tacgccttga gcagggcaat ctcctctctc tcggccaacg
     8581 acaggtaacg ccccgagacc ggggccaagt tgatcggcga cataccccca ttctcgcgga
     8641 accaccgcgt cccgaccgcc ggcgacacgc cgcattccac ggcggcgtcc tcactctgca
     8701 agccgcgggc gatcgacttc cagaaccgct gccgatcctc cctgtgcgcc acattcggcc
     8761 ggccaggcga gcgcatcgcc ggacggatgg cccgatccgc cgcgcgtcgt ttcttgcgtt
     8821 gtcccacagc aactcctcat cattgaggcg ttgcgacgac cccttgaatc caggcagtac
     8881 gcctccgtgg agttcggtgg gaggctcaag gaggccggac ttctgctctc gatgggatcg
     8941 gtggccgacg cctacgacaa cgccctggcc gagtctttcg tggcgacgct caagagcgaa
     9001 ctcttgcacc ggcattcgtg gcccaccaag gagcgtctgc gggtcgccat cttcgagtac
     9061 gtcgaagggt tctacgacag gaagaggagg cactccgctt tgggctatct cagcccggca
     9121 gaatacgagg aaggtagaat gagtgaagtg accgtagcgt agaggtaagt tgtctacctt
     9181 accgggtcaa ctccaattcc atagacgagt ccggattcca tgtctccatg tctccatgtc
     9241 tccatgatgc gcctgagggc gagagcgccc agagggaagc gagcgtatgg caaggttcct
     9301 cggaaccggg gcaagaacca gaccttgatc gcttcaatca ctcttgaggg aggattgagg
     9361 gaggaatggg cgaagctatc tccatcaagg gtgccaccga tgccgaactc ttcgaagcct
     9421 acgttgagga tttcctcgcc tcgacgctca aagtgggaca ggttgtggtg ctcgacagac
     9481 tcggagcgca caggacaagc agagtcaggg aactcgtcga agagcgggga gcacagcttt
     9541 tgttccttcc gtcctactcg ccggactata acccgataga agaagcgttc tccaagataa
     9601 agaacatcgt caggaaggct ggagcgcgta cccgtgaggc gctcgatgaa gccatctcag
     9661 aagcgctctc atcggtcacg ctcgaagacg tggcgggatg gttttcgcac tgcggctacc
     9721 agccacgggg tcaatgttta tgaacaccgc tgtagtcccc cctcgttttg ttctggagac
     9781 cggtatctac cctccaaccc cctgcgtgct acccggcgtc atagtgggaa cgagagtcgc
     9841 gctgtcaggt ggtgtttatt gaagggtgga tctcgccagt agatccccta ttcgctccag
     9901 gccccggatt atgttctggc gcgagttggc gtaggagaag cgcaggtgcc cctctccgta
     9961 gggaccgaac ccggtgccgg agaggcaggc gactccggac tcttcgagga actggtcggc
    10021 cagggcgtcc gcgtcgcggc cggtgtcggt gatccggggg aaggcgtaga aggcgccctg
    10081 cggagtggcg cactcgacgc cgggcaggtc gttgagtccg gcgacgatca ggtctcgccg
    10141 cgcccggaac tccttcagca tctccttcac gctctcctgc gggccggtga gggcttcgag
    10201 cccggcgtac tgcgtcgcgg cgttggtgca ggagttggag ttgacctgaa gagtggcgat
    10261 ctcttcggcc agcggcctcg gcatcacgcc gtatccgagc cgccaaccgg tcatggcgta
    10321 ggtcttggag aagccgtcca ggatgatggt ccgctcgtcg gggccgatgc ccggctcgga
    10381 ggcgatgctg gcgaacccgc cggagtagac gaggcgagag tagatctcat cggagagcac
    10441 gaaaacgtcc gggtgctcgc gcagcatctc cgcgagaccc gcgatgtctt cggccgtcag
    10501 gacgccgccg gtcgggttgg ccggggagtt caggatcacc agcttcgtgc gttcgctcag
    10561 acccgcgcgg aactcgtccg ggtcgagccg gaaaccgttc tcctggcgca gcggcaggaa
    10621 gacgggcgtg ccgccggcga agttgatcat ggactcgtag atcgggaacg ccgggctcgg
    10681 cagcagcacc tcgtcgcccc tttcgacgag cgcgaggatg gcgaagaaca tgatcggctt
    10741 cccacccggc gtgaccacga cctgatccgg ctccaccgcg atacccctgc tgcgcgacac
    10801 gtcggcggcg atggcctccc gcaactccgg gatgccggcg gtaggggtgt aatgggtgtg
    10861 cccgtcgcgc agcgcccggc acgcggcctc gacgatgttc tccgcggtgg ggaagtccgg
    10921 ctcgccgatc tccaggtgga tgacctcgcg tcctcgcgcc tcaagcgcct tcgccttcgc
    10981 cagcaccgag aaggccgtct ccgtccccag acgtcccatc cgcttcgcca gtttcatact
    11041 cgtttacctc tccagattag tcccatcgtc ccgtaacacc atacgcgctc cgctaatact
    11101 ccgccatccg gcgcgtttcc cagaacgggc tagaatatgc cggtcggctt ttcgagaggt
    11161 ttgaatttgg gcgcgcgaaa caacggattg aaaaatgact caaggaagaa tggcctgtcc
    11221 ttcgagcttg gggctttcga cctcgacgga accgtgctgc ggcgggacct cgtgatctcg
    11281 gagaagaccg tcgccgctct cggcaggctg cgggagcgcg ggatgcgtct cgtggtcgcg
    11341 acgggacgcc gctacgagag cgcccgcgag cacgccgggc ggctgggttt cggtgacgaa
    11401 gaccccgtca tctgctacgg cggttcgatg gtccggcgca tgaacggcga gactctcctg
    11461 caccggacgc tgcccacgga cctgagcatc gaggcgttgg agtgggcgtc agagagggga
    11521 ttgcaccacc gcatcctcat ggacgacacg atagtcgtct cccaggaccc gcccacgagc
    11581 ctggaacgcc tctccaaccc cggcgagccg gagaccttct cggtcgagtc tccggctcgt
    11641 tggcttcggg agaccggcgc ggaaccgacg aagctcgtga tcgtggacac ccccgacggc
    11701 gtcgagtgct ggctgcgcga ggctcaggac gctttcgccg gcaggctctt cgttacccgc
    11761 agcctgccgc attacgtgga gatcagcaac ctcgaaggca ccaaatcgaa tgcgctggcg
    11821 ttcctctgcg ataagtggtc ggtcgaaccg gcgcgcacgc tcgcgtttgg tgacgcggac
    11881 aacgacatag atatgctccg ctttgccgga cacggggtcg ccgtcggcgg gatgaccgag
    11941 gaggtccgcg aggtggccga cgccgtcgcg cctccggtga acgaggacgg cgtcgcccgc
    12001 tacatcgaga agcttcttgg aggaatagat gcctgagttg cccgaggtga cggtcatctc
    12061 ggaggacgtg gccgctctgg cgaaaggccg gcaagtgctc cgggcggccg tcttccgtcc
    12121 ggacgttacg aacgtggagc cggaggagtt cacccggcgg ctggtaggga agacgctccg
    12181 gggcacggga cggcgcggca agatcatcgt cctcgacttc ggggatgtta tcgggatcgt
    12241 gcacctcgtc atctccgggc gcgtgctgcg cctcgacgaa tggacgcagc cggaccggat
    12301 gaacaccgcc gtcctggagt ttggtggcgg gctcgtcctg gccttcactc gcctctggct
    12361 cggatatttc gacctctacg agccgggcga ggaggagaag cacccgctca tcgcccggct
    12421 cggcccggac ccgttctcgg aggcgttcac gcccgagtac ctggcggaga tgtttcagcg
    12481 cagggcgagc gtgaagggcc tgctactcga ccagtcggtc gtcgcagggc ttgggaacat
    12541 ctacgtggac gaggtgctct tcgcggccgg agtccaccca tctcgcaagg ccggcaccct
    12601 gagcgtggag gagatacaga ggatccacgc cgccacccgc gacatcctcg gccgcgccat
    12661 cgagcttcgc ggaacgacct tcgactccta ccacgacgct ttcggcgaga ccggcaagta
    12721 ccagcacgag ctgaaggtct ttacccgcgc cgggcagccc tgtccggcgt gcggcactca
    12781 gatagagaag tcccgcgtcg ccgggcgtgg gacgcacgtc tgcccctcct gtcaggcgcc
    12841 gtagccgctt gcgcacaggc gcttccgcta tacttttgcg ggttatgagc cttttctgcc
    12901 aacagagacg cgcgtgagcg cgccggtaga cagtttcctg gaacggcatc ccaacgcggc
    12961 acggaacatc gcccgctacg gctggctcgc gttgctcgtc ctcgcggcgc tactgctcgc
    13021 gggaggcacg gtgctcgcgg gggggaggag ccagcagatc gtctacgcca cctcggacgg
    13081 ctccatcgtc tcgctggagc cggtctccgg ctcctccacc atcctctaca agggcggcgc
    13141 cggacactac gccaccatgc cgggacgcac cggctcccgc agcatctcat tcaccgttct
    13201 gcgcgatgac gggggctccg tgcgcggcaa cctctacggc gcggacatcg tgcgcgagac
    13261 gcgcgcgctg ctccaggacg cccgtccggg ggaggtgttc gcctatccgg actacgccgc
    13321 cgaccgcgag tgggtgatgg cgaaccgctt caccagggat acgccgccca acgtcgaggt
    13381 cttcaccgcc agcgccgcgg cgagccgcct ccttgagccc gacacgcccg gagcctcggc
    13441 cctcgccgga ccgtcctgga cggcggagcg ctccatctac gcctggagag tcggccagga
    13501 gaagacttcg ctcacggcct acaacttctt cgagcggcgg caggcggccg tctacgagac
    13561 gaaggacgtg gtcggaccgg cctcctacta cttcgacggc aacgccttcc tgttcgccga
    13621 gcgtccgcgc ggcgcgggtc tcgaagactc gcggataaag gtgcttgtgg gtaccgggac
    13681 ggccaaaatc tccggtgtcg gagaactggg actctacgac ccgtcttcgc cagcgaccgt
    13741 cctgggaggt aagatctccg tgatctggac cgacggcgag aagaccggta tcgggatgat
    13801 cgacccgcgg ggctggacct tcagcaagac cggcatagag gtcgcggccg ggagccgcga
    13861 cccgcgcctc agcaacgacg gtctctacgt cgccaccacc gactctgcgg gcaaagagat
    13921 cacggtccgc aggctggacg acggcggcgt cgtgcggcgc atccgcgacg cgcagccgcc
    13981 ggagaccgcc atagatcgga tgcgcgaggc cggcatcaag gtccctgaag agaccgggtg
    14041 gctcgcgccg ccggactacg gctggagaag ccttgaagac acgtagcccg cagccgccga
    14101 aggtccagaa ggccgccggc gcccgccggc ggctcgggcc gcgcgagctg gtcatctgct
    14161 gcgccgtcgt ctacgcggtg ctcatctccg ggctctcgat gtacaagcac gagacctacg
    14221 cctcctcgcg ctttgacctg gggaatatgg atcaggcggt gtggaactcc gcgcacgggc
    14281 gcatcctgga ggcgacggac gagtacggcg agataacatc ccgtctcaag aaccacaccg
    14341 acttcctgct gctagccttc gtcccgctct actggatctc gccgagcccg cactggctgc
    14401 tcgtcaccca ggcggtggtc gtcggcatcg gtgcggtgcc cctgtattgg ctggcgcgcc
    14461 ggttcctcgg gtacgagctg cccgcagccc tcctcgccgt cgcgtacctc ttcaaccccg
    14521 gtctccagtc ggcaaacctc ttcgacttcc acgcccagat gatggccggc acgttcctgc
    14581 tcttcgcctt ccactacctg ctggagcgcc gcctgctgcc gttcctggct ttcgcgacgc
    14641 tggccgcgct caccaaagag gggatcgtcc tgatcgtcgg catgatgggc ctctacgtcc
    14701 tgctggtaga gcggcgcccg cggtggggga tacccgtatt cctggcgagc gccggctact
    14761 tcctgctgac gatgctcgtg atcatcccgt tcttcaacac cggcggcgcg agcgagctgg
    14821 tcgagggacg ttacgcggcg tttggcggct ccctcggcgg cgtcgtcagg accaccctgg
    14881 ccgagccgat cttcactatc tcctacgtcc tctcgcagca gaaggccctc tatctgacct
    14941 acctcaccgg catgacctgg ttcttcgggc tcttctcccc gacgacgctc gtgctccccc
    15001 tgccggacct cgccgtcaac ctgctctccg accggcccca gatggtcaac atccgctacc
    15061 attactcggc cacgatcatg ccgttcacct acgtcgcggc ggcggcgggc ctcgccaacg
    15121 tgctgtactt cttccggcgg gccgcgaact ggcgccgtcc gttctcgtgg cttggccggc
    15181 tggcgaggag agaggtctcg tccgagcgca cggccgtcgt cctcacggcg gggatgctgc
    15241 tattcgccgc gcagatggac tacgactacg gtccgttgcc cgtgtttcac tctcccggca
    15301 acttcgcctc cgtcatcaag cccgcgccgg aggagcacct gcgcgccctc gacgaggccg
    15361 tagccatgat cccggacggc gcgaaagtct ccgcgagcaa tcagatagga ccgcacctct
    15421 cctaccggcg ctacctctac ctcttcccgg acctggggga cgcccggtac gtgatcctgg
    15481 acgagaccga accgggctac gatacctata tcaacccggt cctcaacct
//
LOCUS       DASSMB010000164        19314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_39977, whole genome shotgun sequence.
ACCESSION   DASSMB010000164 DASSMB010000000
VERSION     DASSMB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 19314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19314
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39977"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFJ72_09675"
     CDS             complement(<1..261)
                     /locus_tag="VFJ72_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7479766.1"
                     /translation="MQRRADPLDLLAVGETLVDFISVEQTDWLRNATTFRRYLGGSPA
                     NIAVNVAKLGGASAVVSKTGIGAFGQFIKSELQRYGVSIDYLI"
     gene            complement(424..858)
                     /locus_tag="VFJ72_09680"
     CDS             complement(424..858)
                     /locus_tag="VFJ72_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7479767.1"
                     /translation="MGQWKQILVGVDGSPASRKALGWAGEEAVRHGSDLVVLTAWLPT
                     PPPLAGTSSTLPTHGSADAREASMQLLMETIRDVLGEDPPVLVQPQLKEGNAAKVLID
                     LSEDADLLVVGSRGHGGFVGLLLGSVSQHVAAHAKCTVVVVR"
     gene            complement(960..2849)
                     /locus_tag="VFJ72_09685"
     CDS             complement(960..2849)
                     /locus_tag="VFJ72_09685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479768.1"
                     /translation="MAIAAQEGSPPDAQSVDLERKPPLSFRAILLMNVGFFGIQYSFG
                     LQQTAVNPLFQFIGADPAELPLLNLAGPITGLIVQPLIGAISDRTWSPRWGRRKPFFI
                     VGAIGCSVFLFLFPWVSALWMAFILLWMLDISNNTAMEPYRAFISDKLPKSQLARGFL
                     TQSLFVGAGAVTANLSLFVFQQLITGATGAGVPIWVFWAFWIGTVCSIGSVLISVLST
                     KEIPPTEEELAELRAKPRGLVAAVTEIASAVRAMPLGMHKIGVVFAFQWYAMFIYWQF
                     VATSIGKTAFNVTPDDKGFQEAAGWVGLLNGSYNLVTVFAALALIGLAARFGAKWIHA
                     AALAGAAVGLVALSQIGNQYLLFVPMILLGIAWASMMGIPYIIVASMVPRERTGVYMG
                     IVNMMIVVPMLIETLTFGWIFENLLGGSGPNAIMFAGALLFLGALAMLTVNPPKETEE
                     SAIMPLGAPRHISVYDRVVVGSDGTPTSLKTVGHAAGVAAAADAQLVIVSAYSSEPQR
                     VTATGKRVPGVRRLLYGEEAARAALRTSVKQLDAERVRNIETRIAEGDPAKALLDVAG
                     TNPANVIVVGNRGLGAADGQLLGSVPGDVAKNAVCDVLIVQTSDAEDQITNGNGQPPG
                     PNGQT"
     gene            complement(2864..3178)
                     /locus_tag="VFJ72_09690"
     CDS             complement(2864..3178)
                     /locus_tag="VFJ72_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479769.1"
                     /translation="MPGFQVYTIDVIVLISCLAASRAIPIRPTLRKEDAKDYFLGGCN
                     FIWPLTAFTPPFYLRSGVFTMPEFLAKRCDSRNRLPRAPTKLSKSQNTSAKPTMTDGA
                     NT"
     gene            complement(3625..4092)
                     /locus_tag="VFJ72_09695"
     CDS             complement(3625..4092)
                     /locus_tag="VFJ72_09695"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7479770.1"
                     /translation="MDTVDAGSRSGERALRLVDEFAAFGPSYMKWVKSRLQESGLTYA
                     RMRLLGALHCGGPQIMTSISDELGVTRRNITALVDAMEEEGLVRRQPHPTDRRATVIE
                     MTERGSETANRMYDEHRAAVAELFADLSEEDKRELIRLLGVLRGALRGKGFTC"
     gene            4236..5102
                     /locus_tag="VFJ72_09700"
     CDS             4236..5102
                     /locus_tag="VFJ72_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7479771.1"
                     /translation="MKSITVGEVEITALTDISGPFFRLDQIFPGIPPNQWEPYFHRYS
                     WAFADRNTLQGRVGAYLLRSPAHTVLVDTGVGPGGMGTHGKLLEELASDGVRLEDIDT
                     VFLTHLHGDHLGWSVKAGGEPVFPEARYVTQALEWEMSAPYLGRPFTGLDDLGVVELL
                     DGEEAVTDELTAIPTPGHSPGHASLLVSSGGEQAIVAGDAIVHPAQATEPTWNIHFDM
                     DKEQAAFTREMLLEWLEADGMVVAAGHIPGSGFGRIVREEGRRYWRRLETADIETLPP
                     NVSLPEEANRES"
     gene            5092..5259
                     /locus_tag="VFJ72_09705"
     CDS             5092..5259
                     /locus_tag="VFJ72_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479772.1"
                     /translation="MRANEEVVEARNPGAASGAPERARQGPENEEWAWMVDEDPVFYL
                     DEPGAPSARGE"
     gene            5378..6247
                     /locus_tag="VFJ72_09710"
     CDS             5378..6247
                     /locus_tag="VFJ72_09710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7479773.1"
                     /translation="MENVNGGMGGKVVLITGGTSGIGKAAATALAAMGAEVVITGGNA
                     ERGEKALADVRADSRNDSVELMLADLSKQSEVRRLADEFLERHDRLDVLVNNAGVVQS
                     KRTEAIDGIETTLAINHLAPFLLTNLLLDRLKESAPGRVVTTSSEAQRWGSMDFDDLQ
                     SRRKYRAMAVYGMTKLANIMFTYELAERLRGSGVTANCFHPGGVNTNFGNNNRGFATL
                     LFRAAKPFMRTPDQGADTLIWLASSPEVEGISGKYYSDRKEIEAKDIARDPKARKKLW
                     EMSEELTGLKVTA"
     gene            6247..6984
                     /locus_tag="VFJ72_09715"
     CDS             6247..6984
                     /locus_tag="VFJ72_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7479774.1"
                     /translation="MGRERGITAENLVREFRKGPRAVDGVDLRVEPGEVYGFLGPNGA
                     GKSTTVLMLTTLLPPTSGTARVAGYDIVREGPQVRASIGAALQEAALDPFLTGREHLH
                     LQAALHGLPRKERKRRGAALLERVGLEEAADRKVRGYSGGMKRRLDLALALVHEPRIL
                     FLDEPTTGLDPQSRSALWAEVSRLARDEGVTVFLTTQYLEEADVLADRVGIIDRGKIV
                     AEDTPRSPESRDRSSQRRGHPRRALRT"
     gene            6935..7240
                     /locus_tag="VFJ72_09720"
     CDS             6935..7240
                     /locus_tag="VFJ72_09720"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479775.1"
                     /translation="MGRPSVEATPAERSERDAVARVLGRFGEEIAAQPGAVAVRLSGG
                     AAGLADVVRALDKEDLKVSNLRLDEPSLDDVFLAKTGRSLEGAGDSEEDGEMTEAQS"
     gene            7237..8013
                     /locus_tag="VFJ72_09725"
     CDS             7237..8013
                     /locus_tag="VFJ72_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479776.1"
                     /translation="MTGSSFFTQVLALARRSTVRTLRQPAVIISALVFPMMFFSINAS
                     GLTAATKIPGFPADSYLDFAFAFPLIQASLFGAITAGSDLARDIESGFFDRLSLTPMR
                     PVALLTGMLAGVVALGLLQGVVFLAFGLLLGVNISSGVGGIFVIVLLTVLVALGFGGV
                     GAILAIRTGSVEAVESAFPLFFVAIFMSSINLPRNLIEQDWFRFIATINPISYLVEGI
                     RSLVITGWDAQALTLGFGCAAAIVVLAISGAAASMRTRVA"
     gene            8015..8788
                     /locus_tag="VFJ72_09730"
     CDS             8015..8788
                     /locus_tag="VFJ72_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479777.1"
                     /translation="MGGVRAFFGVARAVAGRQVYKYFTNPAFLAPALFPLFFFVAFAG
                     GLTRVGDIPGFDYAPGYIAFQYVWALLQAVTMGGAFTGFSIAGDFENGFARRLMLAAP
                     NRYGIILGYTLASLVRATMTGSLVTVIALITGMPISGGFDFFGLIALAALANIAATLF
                     GAGVAMILRTQEAGPIIRTPIFLVLFLAPVFVPLNLLTGWISTIARLNPFTAILEAAR
                     GFLAGDPTNVIQAFAISLTLILILTFWAVYGLRRAEKAG"
     gene            8863..9267
                     /locus_tag="VFJ72_09735"
     CDS             8863..9267
                     /locus_tag="VFJ72_09735"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET7479778.1"
                     /translation="MTKAHSSVYRATGGKFAGTMGSGRPILLLNTIGRRSGEKRTHPL
                     LYLADGDDMVIVASAGGAPKHPAWYLNLKANPETTVEVGDREIRVRAEEAGPEEKARL
                     WPKVVEMYSGYAGYQEKTEREIPLVILHPVGG"
     gene            9300..9512
                     /locus_tag="VFJ72_09740"
     CDS             9300..9512
                     /locus_tag="VFJ72_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7479779.1"
                     /translation="MEDGLKTVTKEELKGKIDRGDEFVLLEVLSEESYRRAHLPGAIQ
                     FQDMDRIPDLLPDKSAEVVAYCSNYT"
     gene            9646..10326
                     /locus_tag="VFJ72_09745"
     CDS             9646..10326
                     /locus_tag="VFJ72_09745"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET7479780.1"
                     /translation="MATTQTVSFRGEEDQLQGYLARPEGTGPFPGVVVIHEVFGLNEN
                     MRDIARRFADAGYAAFAVDLFTGRSRAVCMARFMGGMLRGTPERFGTGDLKAALGVLA
                     QQSYVDGERIGAIGFCMGGGFAVAWACTDDRLKAIAPFYGANPRPLSAVARSCPVVGS
                     YPEKDFTAKSGRKLDAELARHGIPRDIKVYPEAKHSFFNDRGKSYDPAASEDAWRRTL
                     AFFGEHLG"
     gene            10420..11124
                     /locus_tag="VFJ72_09750"
     CDS             10420..11124
                     /locus_tag="VFJ72_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479781.1"
                     /translation="MPVIEASGLTRTYGRGESRVEALVDVDLTVEPGEWVAVIGPSGS
                     GKSTLMNLLGLLDRPTSGSYALNGREVSSLRGGELARARRELVGFVFQSYNLLPRQSA
                     RKNVELPMVYAGVGGRERKKRALAALEKVDLLDRARHRPPELSGGQKQRVAIARALVN
                     EPAMLLADEPTGNLDSASGTAILDLFGELNAGGVTLIVVTHDPEVARRADRTVEIRDG
                     RVFSDERSGTAGAERR"
     gene            11121..12311
                     /locus_tag="VFJ72_09755"
     CDS             11121..12311
                     /locus_tag="VFJ72_09755"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479782.1"
                     /translation="MKTLRIAGMGLAGLLVNWGRTALTMLGIIIGVAAVVLLTALGNG
                     IQKSISGQINDLGPNLITVTPGSENGDGDGDGGPFGAAQVSTLKPSDTRDVAKLSGVA
                     AVSSSVSIVAPVDRKSVSFSGVDPSYGKIRSIKLAAGRFVKDRGEVVLAASAAKDLLN
                     KEPKAAVGRKLEIGAKKYEVVGVAKKADPGFGPPVPDTSYMKTDDALKLSGSKTVGQI
                     LVQAESARSVDGVVDRIKGSLKKSHDGAKDFSVFTQKQLLSTFTQITDQLQIFLAGIA
                     GISLLVGGIGVMNIMLVSVAERTREIGVRKALGATNADVLFQFLLEAVLLALIGGLVG
                     VAVGIGGSILLPELLKDLPPAVVTPQEVGLAFGVSALIGVVFGVLPAYRSARLAPVEA
                     LRRE"
     gene            complement(12333..13190)
                     /locus_tag="VFJ72_09760"
     CDS             complement(12333..13190)
                     /locus_tag="VFJ72_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7479783.1"
                     /translation="MDRLIESLIGRLVLATTTVFKKPKLAVFLYALALVGVFRSPDRN
                     RPVSYSGAATPEDVPSPKREDPTAFVLAHLEKAGLRTSERVFVSGEQIYSPGEPAEQL
                     HFLRSGTVRLYKLYGGYKEATVGLLKDSGMFGRPDLEESGFQDDFAEAMTTCTVVSVR
                     KSALTWLAMRDPKVSMALFSAFSERIRLSEQFSTVLLPREVSSRLAALLLSLSERVGV
                     EEEEGTLIDLRLTHQILADMIASTREAVSKAMSEFQREGLIARSGRGGFVVLDPLSLR
                     ERAEGAFQP"
     gene            13488..13931
                     /locus_tag="VFJ72_09765"
     CDS             13488..13931
                     /locus_tag="VFJ72_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7479784.1"
                     /translation="MVEKLSREEALQGIHPVLPSLAVAFKRMVSTIERETGVAGMKWF
                     LLTVLGRHDGLSQGEFTQEYEMEPSRVTRTAQSLEGDGFIRRERDSEDNRVMRMYLTD
                     KGREQLRKLPEINDELRRRVHSVLSEEEFEELRRMLTLLAGAMKD"
     gene            13981..15894
                     /locus_tag="VFJ72_09770"
     CDS             13981..15894
                     /locus_tag="VFJ72_09770"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET7479785.1"
                     /translation="MSATVNQGEQTGRRETVLAVAGLMLALFLVALDQTVVGTALPQI
                     IADLDGFEHYAWVTTAYLLASTAMIPVIGKLGDIYGRKWFIIAGVALFLAASALCGAA
                     WGMTELILFRGLQGLGAGMIFSNIFTSVADIFPDPARRAKYQGLFFSVFALTSVIGPT
                     LGGWITDNLDWRWVFYINLPLGIFSLFALPVVLHQGARRYGVKIDYLGAATITASVVS
                     LLLALSWVGEGYDWDATRVIIGFVVAGILLAVWIPIELGAAEPIIPLSLFKSRVFSSA
                     ALLMFMVGIGMFGIILYTPLFVQGVLGKTATGSGTVLTPLVLTMTAMGVITGQIVARV
                     KRIKPFMIFGAVVMTFGVYLLTTLDTDSTQTTVALFLMVVGFGLGPLMPSATLAVQST
                     VERRLLGVATSATQFIRSLGSTVGTAVIGSLVTSGYADHLKDNAPAQAPGRLINALQD
                     PNALVSDQARNALEKVASAFPGGQQLVDQILQVARKGLSDSIHDGFVFTLVAVAFAIL
                     AAVLMKNIKLGEGSAAPAAAPAPASNGRQESLLTGITLEYLARRIETANGDSPNLIAA
                     ASSLVPGEEGSPRERARLAVDQVLRPLALQALSASVERNGTNGTKMNGAAPNGATPDV
                     KGPAEERVHPESV"
     gene            15946..17670
                     /locus_tag="VFJ72_09775"
     CDS             15946..17670
                     /locus_tag="VFJ72_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479786.1"
                     /translation="MTAGQDHALDNRKWLVLSAVSIGTFLSTLSSSIVNISLPTIAHD
                     FGVGIADIEWIVVAYLLTAGSLLLTFGRLGDILGYKRVYIAGFALFTVTSVFCGLAQG
                     VGELTIFRVLQGIGFGMVQAIGPAIITSVFSASERGRALGLTAVSISLGLTLGPTLGG
                     VLTEWASWRWIFFVNLPVGLAGILWSWRVLPGGRNAAAGRFDPAGAFLVGTSMFCLLL
                     ALVEGEDWGWDSFRILGLFVAFAVLGAAFVITELRVRQPVFDLRLFAIRTFSADNVSL
                     LIAFLALFVATFLMPFFLQRGQGFSVLEAGLLLTPLSLTTMVAAPISGTLSDKIGSRI
                     IATAGLIVMGLGLAALARMDAGTGGWDVVWRLAVVGLGLGMFSSPNNSSVLGSVPRSR
                     LGTASGMVAQMRITGQVLGIAAGGAILASRVSDHARELTGSMPQALVQRDALILSIND
                     ALYFAAAVCVLGALTSLVRGKRETGEAEPSSGSESSRALAEVSDRELLLTGLVLAHVA
                     RRIETANGSSPNLIRAASSLVEPNGEVSERARALRAGEEVLKPISRTLMLTYLVRQIT
                     NPERRRLP"
     gene            17667..18155
                     /locus_tag="VFJ72_09780"
     CDS             17667..18155
                     /locus_tag="VFJ72_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479787.1"
                     /translation="MTKYIIGAVGALMAALAGAWLMLAPFALAYQNSGADWADPTYVD
                     FWTGLPLVVISVVGLILFILGLVEELRRRGILQRPYAVPAGQPQPAGAPADPTGNATG
                     QQGNMEQVLLPLITAMLKDMQERDHARDNERDSKQEEVHRTAPNNAQQPRSVGEEGSV
                     KR"
     gene            18155..18415
                     /locus_tag="VFJ72_09785"
     CDS             18155..18415
                     /locus_tag="VFJ72_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479788.1"
                     /translation="MAPQAKVGIITTIISFAAGVWIFLAPFIVEYQKVGQDWIDATKN
                     DLWTGGALISISALTLILFTAFALRDASRAAERQDASTTDVS"
     gene            19001..19091
                     /locus_tag="VFJ72_09790"
     tRNA            19001..19091
                     /locus_tag="VFJ72_09790"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19036..19038,aa:Ser,seq:gga)
BASE COUNT         3405 a         6124 c         6384 g         3401 t
ORIGIN      
        1 gatcaggtag tctatggaga cgccgtagcg ctggagctcg ctctttatga actgcccgaa
       61 cgcgccgatg ccggtctttg agaccactgc cgaggcgccg ccgagcttgg cgacgttgac
      121 cgcgatgttg gccggagagc ctcccaggta acgccggaag gtcgtggcgt tccttagcca
      181 gtccgtctgc tccacggaga tgaaatccac cagcgtctcg cccacggcga ggaggtcgag
      241 cgggtccgcg cgtctctgca tgctcaccag cctaccgtta cgggttctct gagtccaagg
      301 cggatggtct cctccggaag tatcccgcct ccgccaactc gttcagggcg tgcatcgatg
      361 cgaaacggcc cagcgtctcg actcggcggc gcccacgagc gtgcctgagc ctgactcggc
      421 ttgttagcgg accacgacta ccgtgcactt ggcatgcgcc gcgacgtgct ggcttaccga
      481 tcccaggagc aacccgacga acccgccgtg tccgcgagag ccgaccacga gcagatcggc
      541 gtcttccgag aggtcgatga gcactttcgc cgcgttgccc tccttgagct ggggttgcac
      601 gagaaccgga gggtcctcgc cgagcacgtc ccgaatcgtt tccatcaaca actgcataga
      661 cgcctccctg gcatcggctg acccatgcgt gggtagcgtg ctggacgtcc cggcgagtgg
      721 cggcggcgtc ggcagccagg ccgtcaacac gacaagatcc gagccgtgac gaaccgcctc
      781 ctcaccggcc catccgagcg ccttccgact ggccggagag ccgtcgacgc caacgagaat
      841 ttgcttccac tgtcccatcc gacgctcctt taacgggtct ggacctgacg aaaaaacggt
      901 cgcttctcaa tggtggcgcg gcatagttgc agtcgcgcca ccatcgggag cgatctgagc
      961 taagtttggc cgttcggacc ggggggttgt ccgttcccgt tcgtgatctg gtcctccgca
     1021 tcggatgtct ggacgatcag aacgtcgcag accgcgttct tcgctacgtc gccgggcacg
     1081 gagcccagga gctgaccgtc ggcggcgccg aggccgcggt tgccgacgac gatcacgttc
     1141 gccgggttcg tccccgcgac gtcgagcagg gcctttgccg gatccccctc cgcgatccgc
     1201 gtttcgatgt tccggacgcg ctctgcatcg agctgcttca ccgacgtgcg gagcgccgca
     1261 cgggcggcct cctcgccgta gagtaaccgc cggacgccgg gcacccgctt gccggtggcc
     1321 gtgacgcgct gtggctcgga gctgtacgcc gagacgatga ccagttgcgc gtccgcggcg
     1381 gcggcgacac cggcggcatg accgacggtc ttcagggagg tgggcgtccc gtcggagccg
     1441 accaccacgc ggtcgtagac cgagatgtgc cgtggcgccc ccagcggcat gatcgcggac
     1501 tcctcggtct ccttgggcgg gttcaccgtg agcatggcga gggcgcccaa aaacagcagc
     1561 gcgccagcga acatgatcgc gttgggtccg ctgccgccca gaaggttctc gaagatccac
     1621 ccgaacgtca gcgtctcgat gagcatcggg acgacgatca tcatgtttac gatgcccatg
     1681 tacacgccgg tgcgttccct gggcaccatg ctcgcgacga tgatgtacgg gatgcccatc
     1741 atgctcgccc aggcgatgcc gagcaggatc atgggcacga acaggaggta ctggttgccg
     1801 atctgcgaga gcgccaccag ccccaccgcg gcgcccgcga gggccgcagc gtggatccac
     1861 ttggcgccga accgggctgc caggccaatc agggccagcg ccgcgaagac ggtcacgagg
     1921 ttgtaggagc cgtttagcag accgacccac ccggcggcct cctggaagcc cttgtcgtcc
     1981 ggggtgacgt tgaatgccgt cttgccgatg ctggtcgcga cgaactgcca gtagatgaac
     2041 atcgcgtacc actggaacgc gaacaccacg cctatcttgt gcatcccgag cggcatcgca
     2101 cggaccgcgc tagcgatctc ggtgacggcc gccaccagac cccgcggctt ggctcgcagc
     2161 tcggcgagtt cctcctccgt cggcgggatc tcctttgtcg acaggacgga gatcaggacc
     2221 gagccgatag agcagaccgt gccgatccag aacgcccaga acacccagat ggggacgccg
     2281 gctccagtgg cgccggtgat caactgctgg aacacgaaca gcgacaggtt tgccgtcacg
     2341 gcgccggcgc cgacgaacag gctctgcgtc aggaagccgc gggcaagctg ggacttgggc
     2401 agcttgtcgg agatgaaggc ccggtacggc tccatcgccg tgttgttcga gatgtccagc
     2461 atccagagga gtatgaaggc catccacagc gccgacaccc acggaaagag aaacaggaag
     2521 accgagcagc cgatcgcgcc tactatgaag aacggctttc ttcgacccca acgcggactc
     2581 cacgtccggt cgctaatggc gccgatgagc ggctggacta tcaggccggt gatcggtccc
     2641 gccaggttca ggaggggtag ctctgccggg tcggccccga taaactggaa cagcgggttc
     2701 accgcggtct gctgcaggcc gaagctgtac tggatgccga agaacccgac gttcatcaga
     2761 aggatggccc ggaagctcaa cggcggcttg cgctcgaggt ctacgctctg ggcgtcggga
     2821 ggtgagcctt cttgtgcggc gatcgccatg gaaatcctcc tgtctaggtg ttggcgccgt
     2881 cggtcatggt cggtttggca ctagtgtttt gcgactttga gagcttggta ggcgcgcgcg
     2941 gaaggcgatt acgcgagtcg cagcgtttcg ccaggaactc cggcatcgtg aagacgccgc
     3001 tcctcaggta gaagggagga gtgaaagctg tgagcggcca gatgaagttg cagccgccca
     3061 ggaagtagtc tttcgcgtcc tccttgcgga gcgtcggccg gatcgggatc gcccggctcg
     3121 cggcaaggca cgagatcaag acgatcacgt ctatcgtgta tacctggaat cccggcacac
     3181 ttgctcctcc ctcgactcac ccgtcccttg ctctgccttt attgctctgc ctttaccgca
     3241 gaacctctgc ccgagccgac agggatgtcc cgcaacgcac ccctagagcg ctaccggtct
     3301 tcggagagaa gttgagaatc ggataaccgg gcttctcgac gcgtggtccc gtcccacact
     3361 tccctttgcc agaccgtctc cctgccgtgc tttcgactaa agctttttac cgaaaggtcc
     3421 tagaaaagta acagaggtta tcttctccgt caagagtcct aggcttactg gcgcggacgc
     3481 ggggtggcgt ggggacacaa ctggttgccg gcaagctccg gttcgatgtc ttccggtggt
     3541 gcgcatgggt agcggtacga tcgattacca ggatgcggtg gtcgccgatg atggcggcct
     3601 ctcgagaact tcgcagcagc ggtattagca ggtaaaaccc ttgccgcgca gagcaccgcg
     3661 caacacgccg aggagccgga tcaactcccg cttgtcttcc tccgaaaggt ccgcgaatag
     3721 ctccgccacc gccgcccggt gctcgtcgta catcctgttg gccgtctccg agccccgctc
     3781 ggtcatctcg atcacggtcg cccgccggtc ggtggggtga ggctgccgcc gcaccagccc
     3841 ttcctcctcc atcgcatcca ccagcgccgt gatgttgcgc cgcgtaaccc ccagctcgtc
     3901 gctgatgctc gtcatgatct gaggtccccc gcaatgcaac gcccccagca accgcatccg
     3961 cgcatacgtc aggccgctct cctgcaaccg cgacttgacc cacttcatgt aagacggccc
     4021 aaacgcggcg aactcatcga ccagccgcaa ggcccgctcc ccggacctcg aacccgcgtc
     4081 taccgtatcc atgccgctcc ccacatgcca ctcatattaa caactccttg ttaccaatct
     4141 tactgttgac gtacactgtt gtcaatgtta atgtgtatat atacacagta tccggacgcg
     4201 gcgatgtgct cgtcgggatg gagaggaggc cgggcatgaa aagcatcacg gttggagagg
     4261 tcgagatcac ggctctcaca gatatatccg ggccgttttt ccggctggat cagatcttcc
     4321 ccggcatccc tccgaaccag tgggaacctt acttccaccg ctactcctgg gccttcgccg
     4381 accgaaacac gcttcagggc cgcgtcggcg cctacctgct gcgctcaccg gcccatacgg
     4441 tcctcgtgga tacgggcgtg ggacctggcg gcatgggcac gcacggcaaa cttctggagg
     4501 aactcgcgtc tgacggcgtc cgtctcgagg atatagacac cgtctttctc acgcacctgc
     4561 atggcgacca cctcggctgg agcgtgaagg ccggcggcga gcccgtcttc cccgaagccc
     4621 gctacgtgac gcaggccctg gagtgggaga tgtccgcgcc gtatctgggc cgtcctttca
     4681 ccgggctgga cgacctgggc gttgtggagt tgctggacgg cgaggaggcg gtgacggacg
     4741 agttgacggc catccccacg ccgggacatt cgccggggca cgcgagcctg ctcgtatctt
     4801 cgggcgggga gcaggccatc gtcgcggggg acgccatcgt gcatccggcg caggcaacgg
     4861 agcccacctg gaacatccac ttcgacatgg acaaggagca ggcggccttt acccgcgaga
     4921 tgctcctcga atggctggag gcggacggca tggtggtcgc ggccggacac atcccaggat
     4981 ctgggttcgg gcgcatcgtg cgtgaggagg gtcgccgcta ctggcggagg ctggagacgg
     5041 cggatatcga gacgcttcca ccgaacgtaa gtcttccgga ggaggcaaac cgtgagagct
     5101 aacgaggaag ttgtagaggc caggaatccc ggagccgctt ccggcgcgcc ggagcgtgcg
     5161 cggcagggcc cggagaacga ggagtgggcc tggatggtgg atgaggaccc cgtcttctac
     5221 ctcgacgaac ccggagcgcc gtccgcccga ggcgagtagg acgtggctcc tgttacggag
     5281 catggccggg atagcaagaa tactggggtt tagagcgcgg gtcgcggtgt agactcagga
     5341 gcggccgcga ggcttccccg gaacgataag gagcagtgtg gagaacgtca acggcggtat
     5401 gggtgggaag gtcgtcctga tcacgggcgg aacctccggt atcgggaagg cggcggcgac
     5461 ggcactcgca gcgatggggg cggaagtcgt catcaccggc ggcaacgcgg aacgcggaga
     5521 gaaggccctc gcggacgtcc gagccgacag ccgcaacgac tccgtggagc tgatgctcgc
     5581 ggacctctcg aagcagtccg aggtgcggcg tctcgcggac gagttcctgg agcgccacga
     5641 ccggctggac gttctggtga acaacgcggg agtcgtccag agcaagcgca cagaggcgat
     5701 cgacggcatc gagacgacac tcgccataaa ccatctcgcg ccgttcctgc tcacgaacct
     5761 gttgctcgac cggctcaagg agagcgcgcc gggccgcgtc gtaaccacct cttccgaggc
     5821 gcagcggtgg gggagtatgg acttcgacga cctgcagtcg cgccggaagt acagggccat
     5881 ggcggtctac gggatgacca agctcgccaa cattatgttc acctacgagc tggcggagcg
     5941 gcttcgcggt tcgggcgtga cggcgaactg tttccatccc ggcggggtca acaccaactt
     6001 cggcaacaac aaccgcggtt tcgcgacgct gctcttccgg gcggccaagc cgttcatgcg
     6061 gacgccggat cagggcgcgg acacgctcat ctggctggcg tcttcgccgg aggtggaggg
     6121 gatcagcggg aagtactact cggaccgcaa ggagatcgag gcgaaggaca tcgcccgcga
     6181 cccgaaggca cgcaagaagc tctgggagat gagcgaggaa ctgaccggcc taaaggtgac
     6241 ggcctagtgg ggcgcgagcg gggcataacg gccgagaacc tggtccggga gttcaggaag
     6301 ggaccccgcg ccgtggatgg cgtggacctg cgcgtggagc ccggcgaagt ctacggcttc
     6361 ctcggcccga acggggccgg caagtccacc accgtcctga tgctcacgac gctcctgccg
     6421 ccgacgagcg gcacggctcg cgtcgccggt tacgacatcg tgcgcgaggg accgcaggtt
     6481 cgggcatcca tcggggcggc gttgcaggag gcggcgctcg acccgttcct gaccggccgc
     6541 gagcacctgc acttgcaggc ggctctgcac gggctcccgc gcaaggagcg aaagcggcgc
     6601 ggcgcggccc tgctggagcg cgtcgggctg gaggaggcgg cggacaggaa ggtccgcggt
     6661 tactccggcg ggatgaagcg caggctcgac ctcgcgctcg cgctcgtcca cgagccgcgc
     6721 atactcttcc tggacgagcc gacgacgggc ctcgacccgc agagccgcag cgcgctctgg
     6781 gcggaggtca gccggctggc ccgcgatgag ggtgttacgg tcttcctgac gacccagtac
     6841 ctcgaagaag ccgacgtcct ggctgaccgg gtcgggatca tagaccgggg aaagatagtc
     6901 gccgaggata ccccccgaag ccctgaaagc cgagatcggt cgtcccagcg tcgaggccac
     6961 ccccgccgag cgctccgaac gtgacgccgt cgcccgcgtc ctgggccggt tcggagaaga
     7021 gatcgccgcc caacccggcg ccgtcgcggt ccggctctcc ggcggggccg caggactcgc
     7081 cgacgtggtc cgcgctctcg acaaagaaga cctcaaggtc tccaacctgc ggctggacga
     7141 accgagcctg gacgatgtct tcctcgccaa gaccggacgt tcgctggagg gcgccggtga
     7201 cagcgaagag gacggcgaga tgacggaggc gcagtcgtga cggggtcttc gttcttcacg
     7261 caggtattgg cgctggcgcg aaggtcaacg gtgcggactc tgcgccagcc ggcggttatc
     7321 atctcggcgc tcgtcttccc gatgatgttc ttctccatca acgccagcgg cctgaccgcc
     7381 gccacgaaga taccgggctt cccggcggac tcttacctgg acttcgcatt cgccttcccg
     7441 ctcatccagg cgtcgctgtt cggggcgatc acggcgggga gcgacctcgc ccgcgatata
     7501 gagagcggtt tcttcgaccg gctctcgctc accccgatgc gccccgtcgc actgctcacc
     7561 gggatgctgg ccggcgtcgt cgcgctggga ctgttgcagg gcgtggtctt tctcgcgttc
     7621 ggcctcttgc tgggcgtcaa cataagcagc ggcgttgggg ggatattcgt gatcgtgctg
     7681 ctcacggtgc tggtagctct cgggttcggg ggcgtcgggg cgatcctggc gatacggacc
     7741 ggctcggtcg aggccgtcga gagcgctttt ccgctgttct tcgtggcgat ctttatgtcg
     7801 agcatcaacc tgccgcgcaa cctgatcgag caggactggt tccgctttat cgctaccatc
     7861 aacccgatct cctacctggt agaaggtatc cgcagcctgg tgatcaccgg ctgggacgct
     7921 caggccctca ccctcggttt cggatgcgcg gcggcgatag tcgtgctcgc catcagcggg
     7981 gcggcggctt cgatgcggac gagggtagcg taagatgggc ggcgtgcgcg cattcttcgg
     8041 ggtggcgcgg gcggtcgcgg ggcggcaggt ctataagtac ttcaccaacc ccgcgttcct
     8101 ggcgccggcg ctgttccctc tgttcttctt cgtcgccttc gcgggcggcc tgacgcgggt
     8161 gggggacatc cccggcttcg attacgcgcc gggctacata gcgttccagt acgtgtgggc
     8221 gctgctccag gccgtcacga tgggcggggc gtttaccggg ttctccatcg cgggcgactt
     8281 cgagaacggg ttcgcgcggc ggctcatgct cgcggctccg aaccggtacg ggatcatcct
     8341 gggctacact ctcgcctcgc tcgtgcgcgc caccatgaca gggtcgctcg tcaccgtgat
     8401 cgctctcatt accgggatgc cgatcagtgg tggtttcgac ttcttcggcc tgatagcgct
     8461 cgccgccctc gcgaacatcg ccgccactct cttcggtgcg ggcgtcgcga tgatcctccg
     8521 cacccaggag gccgggccga tcataaggac gcccatcttc ctggtcctct tcctagcgcc
     8581 tgtcttcgtc ccgctcaacc tgctgacggg ttggatctcg accatcgccc gactcaaccc
     8641 gttcaccgct atcctcgaag ccgcccgagg cttcctcgcc ggcgacccaa cgaacgtcat
     8701 tcaagccttc gccataagtc taaccctcat tctcatactc acattctggg ccgtctatgg
     8761 actccggcgc gcggagaagg cgggatagaa ggaccacgga ccggaaagga gatgctcatg
     8821 gtaagtctct cgtcgagagc ccaaaacgcg atcggaaagg tagtgacaaa ggcgcactcc
     8881 tccgtctacc gcgccacggg cggcaagttt gcggggacga tgggcagcgg gaggccgata
     8941 ctgctgctca acaccatcgg gcgcaggagc ggcgagaagc gcacgcaccc cctgctgtac
     9001 ctggccgacg gcgacgacat ggtcatagtc gcctcggcgg gcggcgcgcc gaaacatccc
     9061 gcctggtacc tcaatctgaa ggctaaccct gagactacgg tagaggtagg cgaccgtgag
     9121 atccgggttc gggcggagga ggcggggccg gaggaaaagg cccgtctgtg gccgaaggtc
     9181 gttgagatgt actcggggta cgcgggctac caggagaaga cggaacgcga gatcccgctg
     9241 gtcatcctgc atccggttgg cggctgagga cgtagagaac agatgtgaag gagggatctg
     9301 tggaagacgg tttgaagacc gtaacgaaag aggagttgaa gggaaagata gaccgggggg
     9361 acgagttcgt ccttctggag gtgctctccg aggagtccta ccggcgggcg cacctcccag
     9421 gcgcgatcca gttccaggac atggaccgca tcccggacct tctgccggac aagagtgcgg
     9481 aggtcgtcgc ctactgctcc aactacacct gacactcctc gacgcgggtc gtccgggagc
     9541 tgacggccat gggttacgag aacgtctacg actacgaggg cgggaagcag gactgggtga
     9601 acgccggcta cccgaccgag agtggggatt gagaggaagt aacggatggc aacaacgcag
     9661 acggtctcgt tccgcggtga agaggatcag ctccagggct acctggcgcg gccggaggga
     9721 actggtccgt tccccggcgt cgtcgtcatc cacgaggttt tcggcctgaa cgagaacatg
     9781 cgagacatcg cccgccgctt cgccgacgcg ggttacgccg ccttcgccgt ggacctcttc
     9841 accggccgca gccgggcggt gtgcatggcc cggttcatgg gcgggatgct gcgcggcacg
     9901 ccggagcggt tcgggaccgg ggacctgaag gcggcgctcg gggtgctcgc gcagcagtcc
     9961 tatgtggacg gagagaggat cggggcgatc ggcttctgca tgggcggagg tttcgccgtc
    10021 gcctgggcct gcaccgacga ccggctcaaa gctatcgctc cgttctacgg cgcgaaccct
    10081 cgtccgctct cagcggtggc gcggtcgtgt ccggtcgtcg gctcctatcc agagaaggac
    10141 ttcaccgcga agtccgggcg caagctggac gcggagcttg cccgccacgg catcccgcgg
    10201 gacataaagg tctacccgga ggcgaagcac tcgttcttca acgaccgggg caagagctac
    10261 gacccggccg cctccgagga cgcctggcgc cgcacactgg cgttcttcgg ggagcacctc
    10321 ggctagataa gacttcgtaa tgatcggacg cgcaactttt ccggggcgcg gacgtaatcg
    10381 ggagcggagc cggagacacg gacgatagag gagcgtcgga tgcccgttat agaggcgagc
    10441 ggacttacca ggacctacgg gcggggcgag agccgggtcg aggcgctcgt ggacgtggac
    10501 ctgacggtgg agcccggcga gtgggtggcg gtgatcggac cttccggctc cggcaagagc
    10561 acgctgatga acttgctcgg cctcctggac cggcctacct ccggctccta cgccctgaac
    10621 gggcgcgagg tgtcgagcct gcgcggcggg gagctggcgc gggcgcgccg ggagctcgtc
    10681 gggttcgtgt tccaaagcta caacctcctg ccccgccaga gcgcccgcaa gaacgtcgag
    10741 ctgccgatgg tgtacgccgg ggtcggcggc agggagcgca agaagcgggc cctcgccgcc
    10801 ctggagaagg tggacctgct cgaccgggcg cggcaccggc cgccggagct ctccggtggc
    10861 cagaagcagc gggtagccat agcgcgggcg ctcgttaacg agccggcgat gcttctggcg
    10921 gatgagccga cgggaaacct ggacagcgcg tcggggacgg ctatcctgga cctgttcggg
    10981 gagctgaacg ccggtggcgt tacgctcatc gtggtcacgc acgacccgga ggtcgcccgg
    11041 cgggcggaca ggaccgtgga gataagagac ggtcgcgtct tctccgacga gcgttcgggg
    11101 acggcggggg cggagcgccg atgaagacgc tcaggatcgc cggtatgggc ctcgccgggt
    11161 tgctggtcaa ctggggccgc acggccctca cgatgctcgg gattatcatc ggggttgcgg
    11221 cggtcgtgct gctcaccgct ctggggaacg gcatccagaa gagcatctcc ggccagataa
    11281 acgacctggg gccgaacctc atcaccgtaa cccccggctc cgaaaacgga gacggcgatg
    11341 gagacggtgg cccgttcggc gcggcacagg tcagcaccct caaaccctcg gatacgcgcg
    11401 atgtcgcaaa actctccggt gttgctgctg tatcctccag cgtctccata gtcgcgccgg
    11461 tggatagaaa gagcgtctcg ttctccggcg tggacccttc ctacgggaag atccgatcca
    11521 tcaagctcgc cgccgggcgt tttgtgaagg accggggcga ggtggtgctc gcggcctcgg
    11581 cggcgaagga cctgttgaac aaggagccca aggccgccgt cgggcggaag ctggagatcg
    11641 gggccaagaa gtacgaggtg gtgggggtgg cgaagaaggc ggaccccggt ttcgggccgc
    11701 ctgtgccgga cacctcgtac atgaagacgg acgacgctct gaaactctcc ggctcgaaga
    11761 ctgtcgggca gatactcgta caggcggaga gtgcgcgatc cgttgacggc gtggtggaca
    11821 ggatcaaggg ctcactcaaa aaatcccacg acggcgcgaa agacttctcc gtcttcaccc
    11881 agaagcagct actctccacc ttcacccaga tcacggacca gcttcagatc ttcctggccg
    11941 gtatcgccgg catctccctg ctcgtcggcg gtataggagt gatgaatatc atgctcgtct
    12001 cggttgccga gaggacgcgg gagatcggcg tccgcaaggc cctgggcgcg acgaacgcgg
    12061 acgtgctgtt ccagttcctc ctcgaagcgg tactgctcgc cctgatcgga ggtctcgtag
    12121 gtgtcgcggt ggggattgga ggttcgatcc tgctgcccga gctactgaag gacctgcccc
    12181 cggccgtagt gacgccgcag gaagtgggcc tcgccttcgg ggtgtcggcc ctgatcgggg
    12241 tggtcttcgg cgtcctcccc gcctatcgct cggcccgtct cgcgcccgta gaagccctca
    12301 gacgggagta aacgcctgga ggcggaccag cgctacggct ggaaggcgcc ctcggcgcgc
    12361 tctcgcaggc tcagggggtc gagcacgacg aagccgccgc gcccactcct tgctatgagg
    12421 ccctcgcgct ggaactcgct catggctttg gagacggcct cgcgggtcga ggcgatcatg
    12481 tcggccagga tctggtgcgt gagccgcagg tctattagcg tgccttcttc ctcttccacg
    12541 ccgaccctct cggagaggga cagcagcagg gccgcgagcc ggctcgacac ctccctgggc
    12601 agcagcaccg tcgagaactg ctcggagagc cggatccttt cggagaacgc agaaaagagg
    12661 gccatggaca ccttggggtc ccgcatagcg agccacgtca gagcggattt cctcacgctc
    12721 accaccgtac aggtcgtcat cgcctcggcg aagtcgtcct gaaagccgct ctcttcgagg
    12781 tcgggtctgc cgaacatccc gctatccttt aacaacccca ctgtcgcctc cttgtagcct
    12841 ccatagagct tgtagagcct caccgtgccg gagcgcagga agtggagttg ctccgcgggc
    12901 tctcccggag agtagatctg ctctccggag acgaagacgc gctccgacgt ccgcagcccg
    12961 gccttctcca ggtgcgcgag cacgaacgcc gtcgggtcct cgcgcttcgg ggacggtaca
    13021 tcttccgggg ttgctgcacc ggagtaggag acggggcggt tcctgtcggg agagcggaac
    13081 accccgacca gcgccagcgc gtacaaaaag acggccagct tgggtttctt gaagaccgtc
    13141 gtggtagcaa ggaccagtct cccgatcaag ctctctatga ggcgatccac ctctgcatcc
    13201 tcctgagaaa gttcttctgt ctgacctggc actggaagca ataccgcacg ggatccgcca
    13261 gacctctcga atcaccagcc aagcatactc cccggaccca ggcgcaacaa ccacccggac
    13321 aactagcacc tgcccggttt gatcgtccca cagagacctc ccggaacccg ccgcccaacg
    13381 atttttggta gcggtgaata tactcacaaa agtttctaac gcaaaggttg aaactgcaaa
    13441 catcggacat agactatccg ggtcatgagg acgatgaagg tttgaggatg gtagagaagc
    13501 tgagtcggga agaggcgttg cagggcattc acccggtgtt gccgtcgctg gccgtggcgt
    13561 tcaagcggat ggtttcgacg atagagcggg agaccggtgt cgcggggatg aagtggttct
    13621 tgctgacggt acttgggcgg cacgatgggt tgagccaggg cgagttcacg caggagtacg
    13681 agatggagcc ctcgcgggtg acgcgaacgg cgcagtcgct ggagggtgat ggtttcatcc
    13741 gcagggagcg cgactcggag gacaaccggg tcatgcggat gtacctgacg gacaagggcc
    13801 gggagcagtt acggaagctg ccggagatca acgacgagct ccggcgccgg gtgcacagcg
    13861 ttttgagcga ggaagagttc gaggagctac ggcggatgct gaccttgctc gccggagcga
    13921 tgaaagattg aagagcggag cggtcacagt gatgaaactt atatctgatg gagggcggag
    13981 atgagcgcaa ccgttaatca aggcgagcag accgggcggc gcgagacggt tctggcggtg
    14041 gcgggattga tgctggcgct gttcctcgtc gcgctcgacc agacggtcgt gggcacggcg
    14101 ctgcctcaga tcatcgccga cctcgacggg ttcgaacact acgcttgggt cacgacggcg
    14161 taccttctag cgtccacggc catgatcccg gtgatcggga agctcgggga tatctacggc
    14221 aggaagtggt tcatcatcgc gggggtggcg ctatttttgg ccgccagcgc gctctgcggg
    14281 gcggcgtggg ggatgacgga gctcatactc ttccggggtc ttcaggggct cggggccggt
    14341 atgatcttct cgaacatctt taccagcgtg gcggacatct tcccggaccc ggcgcggcgg
    14401 gccaagtacc agggtctctt cttctccgtc ttcgcgctca cgagcgtcat cggcccgacg
    14461 ctgggcggct ggatcacgga caacctcgat tggcggtggg tcttctacat aaacctgccg
    14521 ctcggcatct tctcgctctt cgccctgccg gtggtgcttc atcagggcgc ccggcgctac
    14581 ggggtgaaga tagactacct cggggccgcc accataacag cctcggtcgt ctcgctcctg
    14641 ctggctctct cgtgggtcgg cgagggatat gactgggacg ccacccgcgt catcatcggg
    14701 ttcgtggtcg ccggcatcct gctcgctgta tggataccca tagagctcgg agcggccgag
    14761 ccgatcatcc ctctctcgct gttcaagagc cgggtcttct cttcggcggc gctgctcatg
    14821 ttcatggtcg gcatcgggat gttcgggatc atcctgtaca cgccgctctt cgtgcagggc
    14881 gtcctcggca agaccgccac gggctccggc acggttctca ccccgctggt cctcacgatg
    14941 acggcgatgg gggtcatcac cggacagata gtggcgcgag taaagcggat caaaccgttc
    15001 atgatcttcg gcgcggtagt gatgaccttt ggcgtctatc tcctgacgac gctcgacacg
    15061 gattccaccc agacgacagt cgccctcttc ctcatggtcg tgggcttcgg actcgggccg
    15121 ctgatgccgt ccgctaccct ggccgtgcag agcaccgtcg agaggagact gcttggcgtc
    15181 gccacctccg ccacgcagtt catccgctcg ctaggttcca cggtcggcac ggcggtcatc
    15241 ggctcgctcg tgacctccgg ctacgccgac cacctgaagg acaacgctcc ggctcaggct
    15301 cccggacgcc tcataaatgc cctacaagac ccgaacgcgc tggtgagcga tcaggccagg
    15361 aatgctctcg aaaaggtcgc ctcggccttc cccggtgggc agcagttggt agatcagatc
    15421 ctccaggtag cccgcaaggg actctcggac tctatccacg acggctttgt ctttaccctg
    15481 gtcgcggtgg cgttcgccat cctcgccgcc gtattgatga agaacatcaa gctgggagaa
    15541 gggtcggccg cgccggccgc ggcgcccgcc cccgcctcga acggccgtca ggagtctctg
    15601 ctgaccggta ttaccctgga gtacctcgcc cggcgcatcg agaccgccaa cggagactct
    15661 ccgaacctga tcgccgcggc ttccagcctc gtgcccggag aggaaggttc gcccagggag
    15721 cgcgcccggc tcgccgtgga tcaggtcctg cggccattgg ccctgcaagc cctgagcgcg
    15781 agcgtcgaac gcaacggcac gaacgggacc aagatgaacg gcgccgctcc taacggcgcc
    15841 acgcctgatg tgaaaggacc ggctgaggag cgcgtccacc ccgaaagcgt gtaaggatgg
    15901 gcgctccagc cgttcgatcg tgagagggct agaggagata ggcgagtgac ggccgggcag
    15961 gaccatgctc tggacaaccg gaagtggctg gtgctctcgg cggtgtccat cgggaccttc
    16021 ctgagcaccc tgagcagcag catcgtcaac atctcgctgc cgaccatcgc ccacgacttc
    16081 ggagtcggca tcgcggacat cgagtggatc gtggtcgcct acctgctgac cgccggcagt
    16141 ctcctcctga ccttcggtcg tctgggagac atcctcggat acaagagggt ctacatcgcg
    16201 ggcttcgcgc tctttacggt gacgagcgtg ttctgcgggc tggcgcaggg ggtgggggag
    16261 ctgacgatct tcagggtctt gcaggggatc ggcttcggga tggtacaggc cattggaccc
    16321 gcgatcataa cctccgtctt ctccgcttcg gagcggggcc gggcactggg tctgaccgcc
    16381 gtaagcatct ccctcgggct gacgctcggg ccgacgctcg gcggcgtgct gaccgagtgg
    16441 gcgtcgtggc gctggatctt cttcgttaac ctccctgtcg ggctcgctgg catcctgtgg
    16501 tcctggcggg tcctgcccgg cggccggaac gcggcggcgg ggcgcttcga tccggccgga
    16561 gcgttccttg tcgggacctc catgttctgc ctgctcctgg ctctcgtcga gggcgaggac
    16621 tggggatggg acagcttccg catcctcggt ctcttcgtag cgttcgcggt cctgggagcc
    16681 gccttcgtaa taacggaact gcgggtccgc cagccggtgt tcgacttgag gctcttcgcc
    16741 atacgcacct tctcggcgga caacgtgagc ctcctgatcg ccttcctggc cctgttcgtc
    16801 gccacgttcc tgatgccgtt cttcctccag cgcggtcagg ggttctcagt gctggaggca
    16861 ggactgctcc tgacgccgct ctcgctcacc accatggtcg ccgcgccgat cagcgggacg
    16921 ctctcggaca agatcgggag caggatcatc gccaccgccg ggctcatcgt catgggcttg
    16981 ggcctcgcgg cactcgcccg gatggacgcc gggaccggcg gctgggacgt ggtctggagg
    17041 ctcgcggtcg tggggctcgg cctcgggatg ttcagcagcc caaacaacag ctcggtcctc
    17101 ggttccgtgc cgcgttccag gctcggcacg gcttcgggca tggtggccca gatgaggatc
    17161 accggccagg tcctggggat cgccgccggc ggcgcgatcc tcgctagccg cgtctcggac
    17221 catgccaggg aactcaccgg ttccatgccg caggcgctgg tacagcgtga cgccctcatc
    17281 ctgtccatca acgacgcgct ctacttcgcc gccgccgtct gtgtcctggg cgcactcaca
    17341 agtttggtgc ggggcaagag agagaccggc gaagcggaac cttccagcgg ttcggagtcc
    17401 tcgcgagccc tcgcagaagt atcggaccgg gagctcctgc tcaccgggct cgtgctcgcg
    17461 cacgtggcgc gccgcatcga gaccgccaac gggagctctc cgaacctgat ccgggccgcg
    17521 tccagcctcg tcgagccgaa cggcgaggtc tcggagcggg cgcgggctct cagggccgga
    17581 gaagaggtgc tcaagcccat atcccggacg ctgatgctga cctatctcgt aaggcagata
    17641 accaatcccg aaaggagaag actaccatga ccaagtacat aatcggtgcg gtgggggcgc
    17701 tgatggccgc cctggccgga gcgtggctaa tgctcgctcc cttcgcactc gcctaccaga
    17761 actcgggagc cgactgggcc gacccgacct acgtggattt ctggaccggg ctcccgctcg
    17821 tcgtgatctc cgtggtcggt ctgatcctgt ttatcctcgg gctcgtggag gagttgcgcc
    17881 ggcgcgggat cctgcagcgc ccatacgccg tccccgccgg gcaaccccag ccagccggag
    17941 cgccagctga tcccacgggc aacgccaccg gccagcaggg caacatggag caggtcctgc
    18001 ttccgctgat tacggcgatg ctgaaggata tgcaagagcg cgaccacgct cgggataacg
    18061 aacgtgacag caagcaagaa gaagtccatc ggacggcgcc gaataacgcg cagcagccgc
    18121 ggagcgtcgg agaagaaggg agcgtgaaga ggtaatggcc ccacaggcga aggtaggcat
    18181 aatcaccacc atcatcagct ttgcggcggg agtctggatc ttcctggccc cattcatcgt
    18241 cgagtaccag aaggtcgggc aggactggat cgacgccacg aagaacgacc tgtggaccgg
    18301 aggcgccctc atatccatct cggccctgac cctgatcctc ttcaccgctt tcgccctgcg
    18361 cgacgcatcc cgcgccgccg agcgacagga cgcttcgacg acagatgtca gctagggaat
    18421 gagggacaaa gcataagggc gcggccggac cgcgccccgc ataatgtcgt tatcgatggc
    18481 cgtggtgacc aacagcgctc cggccgccgg ttactacagc ttgtacccgc ttacctcact
    18541 atgagctcgc ggctccgggg tgacatcggc gtcggagctt cggtcagggg cgagagccgt
    18601 agccgcgccg gaacgccgcc atacccaggg acctcgactc gcgtcctggt gacaccccag
    18661 ttcccgccac cgtacccctg cgtcgccgga agagccacgg ttcgccatcc tggatcggac
    18721 cgctatatcc cggtactata gtctgcacga tcgtccggtc acgtcccgct acgccgcctc
    18781 ggatgtctct gtaaagactc atctcatatg ctcctctcaa aaatttgttc aagttcgtag
    18841 ctttgttgtg ctaggttgta gctatgtgcc ataagcatat atttctggag ttggcgtcaa
    18901 gcgcggggta gtgccgtggt gtaaaccgat gtacaagctg gatggtcggg atctcggcga
    18961 catccgcgga gcatctggta gaatttcccg gcactgtcct ggagaggtgt ccgagttggc
    19021 cgaaggagcg cgactggaaa tcgcgtaggc ggtgttaagc cgtctcgtgg gttcgaatcc
    19081 caccctctcc gcttcccttt ctatttgcag gtaaaaatgc gatgacttcg ggagtaagac
    19141 aaagggattt agtatcccag tatcatcacg actgccgtga tcgagagtcc cataccactg
    19201 aggagatcgg agcggatggg tatgaagcgg gcggcgactc ccccgagttg cagaccaacg
    19261 aaagtcatca gggccgtgat agcgccaaag gctcctgccg tgaacacagg ggga
//
LOCUS       DASSMB010000165        12168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1306516, whole genome shotgun sequence.
ACCESSION   DASSMB010000165 DASSMB010000000
VERSION     DASSMB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12168
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306516"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VFJ72_09795"
     CDS             complement(<1..320)
                     /locus_tag="VFJ72_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479789.1"
                     /translation="MSATRMRQDNTIVFVAFEPRPYREIIGETLRTLRPMLYVSVVEP
                     DDLVNQTARLLPALVFCSQKKHLTIPASTDWIEYYFTDEGPLTVLVNGRREETATLTI
                     EGML"
     gene            883..1080
                     /locus_tag="VFJ72_09800"
     CDS             883..1080
                     /locus_tag="VFJ72_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479790.1"
                     /translation="MNERDLILLESAITAIEEAASAVAQEVERDRLRETSLARLSTVE
                     AELNRSRMAIEKILKEEIRQG"
     gene            complement(1085..2233)
                     /locus_tag="VFJ72_09805"
     CDS             complement(1085..2233)
                     /locus_tag="VFJ72_09805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HET7479791.1"
                     /translation="MTTLDLFIVLFVALIVLRGARTGFLAGAFSLAGVVLGASLGSRL
                     APSLLPENESPVYNAGITLAAIVAFAILGDVLARIIGGSLRARLRSQTSMMLDGLGGA
                     ALGLALSLTMVWVVGMFALQSPPLAQVHPAVRDSRILQALNDRMPSELLTDAVADLSP
                     LPEIHGPEAQVAHPDKDIMKDPDILDARSRMVRVTGIACGYGIEGSGWVAAPDLVVTN
                     AHVVAGEILTRVQPGGVGPRRQARVVLFDEKNDIAVLRVDGLGLPSMPLSEPRTGEAV
                     AVMGFPQNGPLDTRAGRTGATRRVISSDAYNRGPIERTVTSFRVYVRPGNSGGPAVNA
                     DGEVVATIFASRSDSRKFGYGIPSQIVQQHIEQATQISGPVSSGDCAN"
     gene            2348..3397
                     /gene="rlmN"
                     /locus_tag="VFJ72_09810"
     CDS             2348..3397
                     /gene="rlmN"
                     /locus_tag="VFJ72_09810"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET7479792.1"
                     /translation="MKAAEFLPEALQVIAERGEPAYRLRQIYGALTRSLVRDWDEATS
                     LPKDLREALAEKVPAAVLDLQKVSRATDGTRKYLFYTHDKHAIETVMIPERGRNTVCI
                     STQVGCAMGCKFCATGLLGIKRNLKAREIVEQVFVAARDIAPERVSNVVVMGMGEPML
                     NYDATLKALKVLNDGDGFNLAARHIALSTSGLVDKIRRFADEPEQFHLAISLHNPFEE
                     ERREIMPVASRHSIPELMDAARYYVDTTRRKLFFEYTLLAGVNDQPRHAEALAELLDH
                     PLYHLNLLRFNWTDTGFSATSKRDAKDFLRRARERGLSATLRPSRGEDIDAACGQLAA
                     KDLRGRPAVPMIPLS"
     gene            3402..3881
                     /locus_tag="VFJ72_09815"
     CDS             3402..3881
                     /locus_tag="VFJ72_09815"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET7479793.1"
                     /translation="MKREPAQRLDPRAKTLWRITNLLGMLPLLLGAIAVGFALFRIVE
                     MPLPVAALPVVVVLALIVLAVGVMPGLRWRRWRYEIRPEEVDLQRGVYWVSRTLVPLA
                     RIQHVDTRQGPLQRRFGLSTVVFYTAAGPNQIPELSAPIAAEVRDRISELTREQDEL"
     gene            3871..5385
                     /locus_tag="VFJ72_09820"
     CDS             3871..5385
                     /locus_tag="VFJ72_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET7479794.1"
                     /translation="MNSEGPASPEPVSPEPRRLHPVGMLIDAVRTVRRWIGALVIPGI
                     AFLASQGFGGRTLALVLLGFVVISVLAAVWGVLRWRATTYGVSGGAFHLRRGVIQKSE
                     RTIPLERVQSVDTVQGVIQRVFGVVEVRIETAGGGGDEPEASLSALGVEAAETLRREI
                     QGSSGEGFRPDTGDEVEPGPEVIRSLSTRDLFIAGATSGQIGVALSLIAVASQTLDDL
                     FSDSFVERIVTTLAPRSISSAILLALAVALFAWLLAIGGTVLSYAGFTLSREGDYLYI
                     RRGLLERREATIPLARIQAVKVVEGVLRQPFGLAMLRVESAGYGKDAGVSTTLFPLLP
                     REEAREILSRAAPEFAVEPPLQKLPRRALRRYVFRAALPVLFLVPVLVLISAVALDSV
                     SGAFLALLLLAPAVLLGWLRYRDAGWALTGERLVVRSRFVARVTTVATRRRLQSRSVV
                     RSPFQRRLGLATLRAQVASGGGGKDVEVVDLAEEAARAIVERLGPRTAAPGPQG"
     gene            complement(5410..6309)
                     /locus_tag="VFJ72_09825"
     CDS             complement(5410..6309)
                     /locus_tag="VFJ72_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7479795.1"
                     /translation="MEDYTNRGLKYGLCILLASLAAVIVAVPVLAQQAPSVTLVGAGD
                     IADCGSRGDEATAKLVKNIPGTVFTLGDNAYDRGTASEFANCYNPTWGQFKSRTRPST
                     GNHDYYSTGARPYFNYFGARAGKPSRGYYSYDRGGWHIIALNSNCDKVGCGLRSAQAR
                     WLRQNLASHPSKCTLAYFHHPLFASGGKTESTAVRPFWRILYNRGADVILSGHAHRYE
                     RFVPQNPDGGRDLDRGIREFIVGTGGEAPEGPINGTDPNSVVKNDKTPGVLKLTLSAD
                     SYSWKFVHIPGKTFTDSGNARCH"
     gene            complement(6396..7367)
                     /locus_tag="VFJ72_09830"
     CDS             complement(6396..7367)
                     /locus_tag="VFJ72_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479796.1"
                     /translation="MPRPTPNSIPTPERIARFPLTGPGGEPVDLARTFHSHGVASLPP
                     MRTDETSGTFEVTLSADGAGSYTVRVSPDGPGHGAVSAAGALCGREGEALLAAVRCVL
                     RLDEDLSGFYARAVEDPELSWVTGGAGRMVRSPSVFEEVIKTICTTNCAWSATERMVG
                     ALVEHLGEPAPDAPQETPYGRSFPTPEAMAEAGEDFYRDVVRAGYRGRYLMALARSVA
                     EGTLDLEALGSSTAEELPDDELELRLLALPGVGPYAAAHVMMMLGRYSRLIFDSWTRP
                     KYARLAGEESVTDEEIRTRFRRYEEYAGLAFWLYLTRDWFPDPLTSG"
     gene            7536..8480
                     /locus_tag="VFJ72_09835"
     CDS             7536..8480
                     /locus_tag="VFJ72_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET7479797.1"
                     /translation="MNSGVGTSQVWSRRGAISVPVLMVIAAICIALVAALPAFAADKS
                     DPQFQVVGGKKVPAGKYPFMASMQFNRAGTTPRQDHFCGATLIAPSYVLTAAHCSVII
                     GNNATQATPIPVNKVRIVVGTTRLNSAQGQARGIAGLSSIHVHPKYTGRNSNYDLAVI
                     RLDNPVKGIKPILLDRSGSNALEKPGSRPTVAGWGDTKAQPATGGTAPRLTASMREAR
                     PPIVSDSKCRRVYASTGDPSLRVTPKLMVCAGKKGVDTCQGDSGGPMFVPTPAGIRQI
                     GITSYGVGCATAKFPGVYTEVSAPPINQFIQRTVPGSP"
     gene            complement(8493..11354)
                     /locus_tag="VFJ72_09840"
     CDS             complement(8493..11354)
                     /locus_tag="VFJ72_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HET7479798.1"
                     /translation="MNLERLYIENYKQLREPVELLPPEGAIGVVGRNGSGKSTLFESI
                     LWAFFGSRGGGPRFANELIPWSGGSAKDKTVVEVTLAVGGDSYTVRRGLRSNQTTAEA
                     QDSTGKTIVTGTTDVTRWVEEHLLRMDRTAFEATFYARQKELKFFAQDDGIGRVRRIS
                     KMLGIHRVEGAQKLLREDRNDLRAEARLIEGRLAESDEDALKEQLEEFKTECKRLEED
                     LERISGEYESAQVELDAARKSRAALDATHREHVRLTNALQMVEGERRRSEDRTEQAER
                     DLAEISTAEEELRRLKPQIQRLPELEKELERLEDDRRRSEQRDRDRKELRTAQARIAA
                     IETEVSEMLEELDSDGESLPGWDGLFELDGAELLGETVVVLGEADERLEQAEERLREL
                     REMEAAHAEHRRGEEELVKARERCAEARRECERISEEIEDLSGGDDLEARERGLREEE
                     EKLRELAAARRGAASANEREARNVDKAREAIASGAEDHCPTCHREFESGEHADIVETL
                     NRQAAAIRRLAAKARDEADQHTTAADAAEEKLQKVTARLGSWRELRETLVRAETAATD
                     REEALQALVEQQRNLETKLGGSPAPSERDLEDAQVRCAYLRSLRDALPGARSLQREHS
                     GLVGRAGELAAELESLAGVSYDPDLYRERREEKASLERVQGRVEELERRVATRPAVER
                     ALEDARTKVREAGKQAETLRGQISALGFDEEEYDDAGVRVAAAEEKTSRLRDAREHTG
                     GEWKDADHKIERVSTELKRLEADRKLANQRAAGAARMDEMDRLFTDFFRSLTARVRPM
                     LEVEASNLVRELTDGRYERMEFDDNYRVKLLDRFDDSYAIERFSGGEADVASLSARIA
                     LSKIIAARGSEALGFLVLDEVFGSLDTERRNNVLLALERLKRSFGQIFIISHVGDVQE
                     SALLDELWMIEEDEDGKSTVRRVEQAPIGAADLLQDAPA"
     gene            complement(11351..>12168)
                     /locus_tag="VFJ72_09845"
     CDS             complement(11351..>12168)
                     /locus_tag="VFJ72_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479799.1"
                     /translation="VYSAHGFDLDYEMVEADGVGVGVTLVPHGAVFGTGAVMPAREAD
                     VNILVTHGMVPGLEAKQHEMGEADLQSAMISAPFDYVALGHYHEFHEHKPNAFYAGAT
                     ERFGFGEVASKPGFAIVEFDGSGLKSVEHVGIEARPMLDLEKIKAREMDSAALTDAVR
                     ERTAGVDLDGAIVRLRAFDVRRGVTSGVDRALLRDLQRRCLNFSLEVHAEELPEERAS
                     GPVSAVFGALEEEFAEFVKVRRERGELEEKFAGEFLEKGRGYLARAASEEQAS"
BASE COUNT         1933 a         4162 c         3917 g         2156 t
ORIGIN      
        1 tccagcatac cttcgatggt cagggtggcc gtctcttcgc gccggccgtt gacgagaact
       61 gttaatggcc cttcatcggt gaagtagtac tcgatccagt cggtcgatgc ggggatggtg
      121 aggtgcttct tctggctgca aaagaccagc gcgggcagaa gccgcgccgt ttgattgacg
      181 aggtcgtccg gctcgacgac ggagacatac aacatgggac gcagcgttcg cagggtctcc
      241 ccaatgatct ccctgtaagg gcgcggttca aaggctacga aaacgatggt gttgtcttgc
      301 ctcattctgg tagcgctcaa cagcctctct ccctggtccc tgctaacggt agtttgtcag
      361 ggtaatgatg gtccgcgcgg ttgcacagcg cctcatacat acagggtatc tttatctctt
      421 tagtttcata gatttagttc tagctcaatt tatatagagg cgcggtgagc aaacgagccg
      481 aaaggaggac ttctaaacat aaacgtcgcg ttccgctcga tagatacata catgacacag
      541 cacgaagtga aggacggcat cgcgaatcca acgaaggcgt gccggtgtcc gcaaagggag
      601 atagcgaaca tggagagaca ggaatcatag attccggagc agtaaacgag aggcgcttca
      661 cctactcaag gactatagag cgctcttaca ggcggtctct ccaggccgga agtcttccag
      721 cacaggatcc gtaggatact ggctgagacc ttctccgtcc cggcccaggg ctacgccctc
      781 cagcaaagcc ttgacgagga cgccgccgtg caaatctggg tgcccgccgg cctgaaccta
      841 ccctctgaac gtctacgagc gacttcagtt agtatccact atatgaacga acgggacctg
      901 atactcctcg aaagcgccat taccgctatc gaggaggcgg catcggcggt tgctcaggag
      961 gtcgagcgcg atcgtctgcg cgagacctcg ctcgcccggc tctccaccgt cgaggccgag
     1021 ttgaaccgca gccgcatggc gatagagaag atcctgaagg aagagatccg ccagggctga
     1081 tcgttcagtt ggcgcagtcg ccgctgctca cgggaccgga tatctgagtg gcctgttcga
     1141 tgtgctgctg gactatctgt gaaggtatgc catacccaaa cttccttgag tccgagcggc
     1201 tggcgaagat ggtcgcgacg acctcgccgt cggcgttcac cgcgggtccg ccggagttgc
     1261 cgggtctgac gtagacgcgg aagctcgtca ccgtgcgctc tatcgggccg cggttgtagg
     1321 cgtcgctgga gatgacgcgc cgcgtggcgc cggtgcgtcc ggcacgggtg tccagcgggc
     1381 cgttctgagg gaagcccatg acggcgacgg cctccccggt ccggggctcg gagagcggca
     1441 tcgaggggag gcccaggcca tccacgcgca ggaccgctat gtcgttcttc tcgtcgaaga
     1501 ggacgaccct ggcctgccgg cgaggtccca cgccgcccgg ctgcacccgc gtgaggatct
     1561 ctcccgccac gacgtgggcg ttcgtgacga ccaggtccgg cgcggctacc cagccggaac
     1621 cttcgatccc gtagccgcag gctatccccg ttacccggac catccgggag cgggcgtcga
     1681 ggatatccgg gtccttcatg atgtccttgt cggggtgggc gacctgggct tcgggaccgt
     1741 ggatctcggg cagcgggctc aggtcggcga cggcgtccgt gagtagctcg gagggcatcc
     1801 ggtcgttgag cgcctgcagg atgcgcgagt ccctgaccgc cgggtggacc tgcgcgagtg
     1861 gcgggctctg cagggcgaac atcccgacga cccacaccat cgtcagggag agcgcgagcc
     1921 cgagagccgc gcctccgagg ccgtcgagca tcatcgaggt ctggctgcgc agccgggctc
     1981 tcagagagcc cccgattatg cgcgccagca cgtcacccag gatggcgaag gccacgatgg
     2041 ccgccagcgt gatgcccgcg ttatagaccg ggctctcgtt ctccggcaag agcgacggag
     2101 cgagtctgga gcccagcgaa gccccgagca ccaccccggc cagcgagaag gccccggcca
     2161 gaaaacccgt gcgcgctccg cggagcacga tcagggcgac gaacaacacg ataaagagat
     2221 caagggtggt catcgccctg attctacagc cggaagacgc cttttaccgc ccgctccctg
     2281 atggattcta cggagcttat ataatcgggg tcgttgtcat tccagagaag caccgaagag
     2341 gagcgcattg aaggccgcgg agttcctgcc cgaagccctg caagtcatcg ccgagcgcgg
     2401 cgagccggcg taccggctgc gccagatcta cggcgccctg acccgttccc tcgtgaggga
     2461 ctgggacgag gctacgagcc tgccgaaaga cctgcgtgaa gcgctggccg aaaaagtccc
     2521 ggcggctgtc ctcgatctac agaaggtctc tcgcgcgacg gacggcacgc gcaagtacct
     2581 cttctacact catgacaaac acgccataga gacggtcatg atcccggagc ggggccgcaa
     2641 cactgtgtgc atctctacgc aggtaggctg cgcgatgggt tgcaagttct gcgcgaccgg
     2701 gctcctcggc atcaagcgca acctcaaggc gcgtgagatc gtcgagcagg tcttcgtcgc
     2761 cgcccgcgac atcgccccgg agcgcgtctc gaacgtcgtc gtcatgggga tgggcgagcc
     2821 gatgctcaac tacgacgcga ccctcaaggc gctcaaggtc ctcaatgacg gcgatggctt
     2881 caacctggcg gcgcggcaca tcgcgctctc cacgagcggg ctggtggata agatccggcg
     2941 cttcgccgac gagccggagc agttccacct cgccatctcg ctccacaacc cgttcgagga
     3001 ggagcgcagg gagatcatgc cggtagcctc ccggcactcg ataccggagc tgatggacgc
     3061 cgcccgctac tacgtggaca cgacccgccg caagctcttc ttcgagtaca cgctgctcgc
     3121 cggcgtgaac gaccagccgc gccacgccga ggcgctggcg gagcttctgg accacccgct
     3181 ctaccacctg aacctgctgc gcttcaactg gacggacacc ggcttctcgg cgacctcgaa
     3241 gcgcgacgcg aaggacttcc tgcgccgcgc gagggagcgc gggctctccg cgaccctgcg
     3301 gccgagccgg ggcgaggaca tagacgccgc ctgcggccag ctagcggcga aggacctgcg
     3361 cggtcgaccc gccgtaccca tgatcccgct ctcctagacg gttgaagcgc gaacccgcgc
     3421 agaggctgga cccgcgggcg aagaccctct ggcggatcac gaacctgctc ggcatgttgc
     3481 cgctcctgct aggcgcaatc gctgttggct tcgcgctgtt caggattgta gagatgccgc
     3541 tgcccgtcgc cgcgctgccg gtagtggtgg tgcttgcatt gatcgtactg gcggtcgggg
     3601 tgatgccggg actgaggtgg cggcggtggc ggtatgagat ccggccggag gaggtagacc
     3661 tgcagcgcgg ggtgtactgg gtgagccgaa ctctcgtgcc gctcgcccgc atccagcacg
     3721 tcgacaccag gcagggaccg ctccagcgca ggtttgggct ctcgacggtc gttttctaca
     3781 cggcggccgg ccccaatcag atccccgagc tctccgcgcc catcgccgcg gaggtgcgcg
     3841 accggatctc cgagcttacc cgcgagcagg atgaactctg agggaccggc aagcccggag
     3901 ccggtgagcc cggagccgcg ccggttgcat ccggtcggca tgttgatcga cgcggtgcgg
     3961 accgtgcggc gctggatcgg cgcgctggtc attccgggta tcgctttcct ggcgagccag
     4021 ggtttcggag gacgcacgct cgccctggtg ctgctcgggt tcgtcgttat ctcggtcctg
     4081 gcggcggtgt ggggggttct ccggtggcgg gcgactacgt acggcgtctc tggcggcgcc
     4141 ttccacctgc ggcggggtgt gatccagaag tccgagcgca cgatacctct ggagcgcgtc
     4201 cagtcggtgg acacggtgca gggggttatc cagcgcgtgt tcggtgtcgt ggaggtgcgg
     4261 atagagaccg ctggaggcgg gggagacgag cccgaagcat cgctctcggc gctcggcgtg
     4321 gaggccgcgg agactctgcg gcgcgagatc cagggctcca gcggagaggg ctttcgtccg
     4381 gataccgggg acgaagttga gcccggcccg gaggtcatcc gttcgctctc gacccgggac
     4441 ctctttatag cgggagcgac gagcgggcag atcggggtag cactctccct gatcgccgtc
     4501 gcttcccaga ccctggacga cctcttctct gacagcttcg tcgagcggat cgtcacgacg
     4561 ctggccccgc gctcgatctc ctcggccatc ctgctggcgc tcgccgtcgc gctcttcgcc
     4621 tggctcctcg ccatcggcgg aacggtcctc tcctacgcgg gcttcacgct ctcgcgcgag
     4681 ggtgactacc tgtacatccg gcgcggcctc ctggagaggc gcgaggccac gataccgctc
     4741 gcccgcatcc aggccgtgaa ggtggtcgag ggcgtcctgc gccagccgtt cggcctcgcc
     4801 atgctgcgcg tcgagagcgc cggctacggc aaggacgccg gcgtctctac gacgctcttc
     4861 cccctgctgc cgcgcgaaga ggcgcgggag atactgagcc gggcggcgcc ggagttcgcc
     4921 gtggagccgc cccttcagaa gctgccccgc cgcgcactcc ggcgctatgt cttccgggcc
     4981 gcgctgccgg tcttgttcct ggtccctgtc ctcgtgctca tctcggcggt ggcgctggac
     5041 tctgtctccg gcgctttcct ggcgctgctg ctcctcgccc cggctgtcct gctcggatgg
     5101 ctccgctacc gggacgccgg atgggcgtta accggcgaac gcctcgtggt ccgctctcgg
     5161 ttcgtggccc gcgtgacgac ggtcgctacc cggcgcaggc ttcagtcgcg gagcgtcgtg
     5221 cggagcccgt tccagcggcg cctgggcctc gcgacccttc gcgcgcaggt cgcctcgggc
     5281 ggtggcggga aggatgtcga ggtcgtggac ctcgccgagg aagccgcgcg agctatcgtc
     5341 gagcggctcg gtccccggac cgcggcgccc ggccctcaag gctgaaaccg ggcgctgaag
     5401 tctctatctc tagtggcagc gggcgttgcc ggagtcggtg aacgtcttgc ccggtatatg
     5461 cacgaacttc cagctataag agtccgcgct aagggtcagc ttcaggacgc cgggcgtctt
     5521 gtcgttcttg acgacgctgt tcgggtccgt gccgttgatc ggcccttcgg gggcctcgcc
     5581 gcccgtgccg acgatgaact cgcggatgcc ccggtccaga tccctgccgc cgtcaggatt
     5641 ctgcgggacg aaccgctcgt agcggtgggc gtggccggag aggatcacat ccgccccccg
     5701 gttgtagaga atcctccaga aaggccggac cgcggtggac tcggtcttgc cccctgacgc
     5761 gaacagcgga tggtggaagt aggccagggt gcatttggat ggatggctgg cgaggttctg
     5821 ccggagccag cgggcctggg cagagcgcag cccgcagcca accttgtcgc agttgctgtt
     5881 cagcgcgatt atgtgccagc cgccgcggtc gtagctgtag tagccccggc ttggcttgcc
     5941 ggccctggcg ccgaagtagt tgaagtacgg ccgggctccg gtcgagtagt agtcgtggtt
     6001 gccggtagac ggacgggtgc ggctcttgaa ctgaccccag gtggggttgt agcagttggc
     6061 gaactcgctg gctgtccccc ggtcgtaggc gttatctccc agggtgaaga ccgtaccggg
     6121 gatgttcttt acgagcttcg ccgtggcctc gtctccgcgg ctcccgcagt cggcgatgtc
     6181 tcccgcgcct accagcgtga cggagggagc ctgctgcgcc aggaccggca cggccacaat
     6241 gactgccgcc agcgatgcta gcaggataca tagaccatac ttgagacccc tattcgtgta
     6301 gtcttccacc ggcgtacccc tcctgatgct gttgtctgta gctgtggtcc cggaagttcg
     6361 ggaccgaaag tatagaacag gaagtatcga gtccgctacc cggacgttag agggtccggg
     6421 aaccagtccc gcgtcaggta gagccagaag gccagccccg catattcctc gtaacgccga
     6481 aaccgcgtcc gtatctcctc gtcggtcacg ctctcctctc cggcgagcct cgcgtacttc
     6541 gggcgggtcc aggagtcgaa gatgagccgc gagtagcgcc cgagcatcat catcacgtgc
     6601 gccgccgcgt acggcccgac ccccggcagc gccagcagcc gcagctccag ctcgtcgtcg
     6661 ggtagctctt cagcggtaga gcttcccaga gcttcgaggt ccagggtgcc ttcggcgacg
     6721 gagcgagcaa gcgccatcag gtacctgccc cggtatccgg cccggaccac gtcgcggtag
     6781 aagtcctcgc cggcttcggc catcgcctcc ggggtgggga aggagcgtcc gtagggggtc
     6841 tcctgcggag cgtccggcgc gggttcgccc aggtgctcga cgagggcgcc caccatccgc
     6901 tccgtggccg accaggcgca gttggtggtg cagatggtct tgataacctc ctcgaagacg
     6961 gaggggctcc ggaccatacg ccccgcccca cccgtcaccc aggatagttc cgggtcctct
     7021 acggcccgcg cgtagaaccc cgacaggtcc tcgtccagcc gcagcacgca ccgtaccgcc
     7081 gcgagcagag cctcgccctc ccgcccgcag agagccccgg cggcgctgac ggccccgtgg
     7141 ccgggaccgt ctggagagac gcggaccgtg tatgaaccgg cgccatccgc ggacagggtg
     7201 acctcgaacg tcccggaggt ctcgtcggtc cgcatcgggg gcagggaagc cacgccatgc
     7261 gagtgaaaag tgcgggccag gtccaccggc tcgccgcccg gcccggtcag ggggaacctg
     7321 gcgatccgtt ccggagttgg gatcgagttc ggagtggggc gcggcatgga aggattgtac
     7381 gcgagcggcg cgggcttcca ccacctgttt cggacccgcg gggttcgcga agcattcggg
     7441 acacacaggg ttgagaagtt ctgagcgcga agttaagctt aaccgtccgc aggttcacag
     7501 gtaagggaga ggttgtccgc gtgaagggtt ttcgtatgaa cagtggtgtg gggacctcgc
     7561 aggtatggag ccgtagggga gccatatccg taccggtgct gatggtgatc gcggcgatct
     7621 gtatcgcgct agttgcggct ctgccggcgt tcgcggccga caagtccgac ccgcagttcc
     7681 aggtcgtcgg cggcaagaaa gtcccggcgg gcaagtaccc gttcatggcc tcgatgcagt
     7741 tcaaccgggc agggacgacg ccgcgccagg accacttctg cggcgctacc ttgatcgccc
     7801 cgagctacgt gctgacggcg gcgcactgct cggtgatcat cggaaataat gctactcagg
     7861 ccacgcccat ccccgtcaac aaggtgagga tcgtcgtcgg gacgacgagg ctcaacagcg
     7921 cgcagggtca ggcgcgcggg atcgccggtc tctcaagcat ccacgtccat ccaaaataca
     7981 ccggcaggaa ctccaattat gacttggctg taatccgtct tgacaatccg gtgaaaggta
     8041 tcaagccgat cctgctcgac aggtccgggt cgaacgcgct ggagaaaccg gggagccggc
     8101 cgacggttgc cggatggggc gacacgaagg cgcagcccgc cacaggaggt actgctccga
     8161 ggctcacggc cagtatgcgc gaggcgcgtc ccccgatagt ctcggacagc aagtgccgga
     8221 gagtctacgc ttctaccggc gacccgagcc tccgggtcac gccgaagctc atggtctgcg
     8281 cgggtaagaa gggcgtggac acctgccagg gggattccgg cggacccatg ttcgtcccga
     8341 ccccggcggg tatacgccag atcggcataa ccagctacgg cgtcgggtgc gcgacggcca
     8401 agttccccgg cgtgtacacc gaggtgagcg cgcctcccat aaaccagttt atccagcgga
     8461 cggtgcccgg ctcgccgtag agccgggtct ttttacgccg gcgcgtcctg gagcaggtcc
     8521 gcggctccga tgggggcctg ctccacgcgc cggacggtgc tcttgccgtc ctcgtcctcc
     8581 tcgatcatcc acagctcgtc cagaagcgcg gactcctgca cgtcgcccac gtgggagatg
     8641 atgaagatct gaccgaacga ccgctttaac ctctccagcg ccaagagcac gttgttgcgc
     8701 cgctcggtgt ccaggctgcc aaagacctca tcgaggacca ggaagcctaa agcctccgag
     8761 ccgcgggcgg cgatgatctt ggatagcgcg atcctcgccg agaggctcgc cacgtcggcc
     8821 tcgccgccgg agaaccgctc gatggcatac gagtcgtcga agcggtccag aagcttcacc
     8881 cggtagttgt cgtcgaactc catcctctcg tagcgcccgt cggtcagctc ccgcacgagg
     8941 ttggaagcct cgacctccag catcgggcgc acgcgggcgg tcaggctgcg gaagaaatcg
     9001 gtgaagagcc tgtccatctc gtccatgcgc gccgcgccgg cggcccgctg gttggctagc
     9061 ttccggtcgg cttccaggcg cttgagttcg gtgcttacgc gctcgatctt gtggtcggcg
     9121 tccttccact cgccgcccgt gtgctcccgc gcgtcgcgca gccgggacgt tttctcttcc
     9181 gcggcagcga cccgcacgcc ggcgtcgtcg tactcctcct cgtcgaagcc gagcgcggat
     9241 atttgaccgc gcagcgtctc cgcctgctta ccagcctccc gaaccttcgt ccgagcgtcc
     9301 tccagcgcgc gttcgaccgc cggccgggtg gcgacacgcc gctccagttc ctcgacccgg
     9361 ccctgcaccc gttcgaggct cgccttctcc tcccgccgct cacggtagag gtccgggtca
     9421 taagagacgc ccgccaggct ctccaactct gcggccagct ctccggcccg tccgaccaat
     9481 ccggagtgct cgcgctgcag gctgcgcgca cccggcagag cgtcgcgcag gctccgcaag
     9541 taggcgcacc ggacctgagc gtcttcgagg tcccgttcgg agggggcggg ggagccgccg
     9601 agcttcgtct ccaggttgcg ctgttgctcg accagcgcct ggagagcttc ttcgcggtcg
     9661 gtggcggcgg tctcggcgcg gacgagagtt tcgcgcagct cgcgccagct ccccagccgc
     9721 gccgtgacct tctggagctt ctcctcggcg gcatcggcgg cggtggtgtg ctgatccgcc
     9781 tcatcccgag ccttcgccgc gaggcgtcgg atcgccgccg cctgacggtt cagggtctcc
     9841 acgatatctg cgtgctcgcc gctctcgaac tcccggtggc aggtggggca gtgatcctcc
     9901 gctccactgg cgatggcctc gcgggccttg tccacgttac gggcctcgcg ttcattggcg
     9961 gacgccgcgc cgcgccgggc ggcggcaagc tcccttagct tctcctcctc ctcgcgcaac
    10021 ccgcgctccc gcgcctccag gtcatcgccg ccggagaggt cttcgatctc ttccgagatg
    10081 cgctcgcact cccgacgcgc ctccgcgcac cgctcgcggg ccttcaccaa ctcctcttca
    10141 ccgcgccggt gctcggcgtg cgcggcctcc atctcccgaa gctcccgcag ccgctcctcc
    10201 gcctgctcca gccgctcatc cgcctccccg agaaccacca cggtctctcc cagaagctcc
    10261 gcgccgtcca gctcgaagag gccgtcccag cccggcagtg actcgccgtc gctatcgagt
    10321 tcttccagca tctcggagac ctcggtctcg atggcggcaa tgcgggcctg ggctgtccgc
    10381 agctccttcc ggtcccggtc gcgctgctcg gagcgccgcc ggtcatcctc caggcgctca
    10441 agctccttct ccagctccgg cagccgctgt atctgcggct tgagccgtct cagttcctcc
    10501 tcggcggtgg agatctcggc gaggtcccgt tccgcttgct ccgtccggtc ctccgagcgg
    10561 cgccgctcgc cttcgaccat ttgcagggcg ttcgtcagcc taacatgctc gcggtgggtg
    10621 gcatcgaggg cggcgcggga cttgcgggcg gcatccagct ctacctgagc ggactcatat
    10681 tcgccggaga tccgctccag gtcttcctcc aggcgcttgc actctgtctt gaactcttcg
    10741 agctgctcct tcagcgcgtc ctcatcagat tcggcgaggc ggccctcgat caggcgcgcc
    10801 tccgcgcgca ggtcgttgcg gtcctcgcgc agcagcttct gggcgccctc gacgcggtgg
    10861 atgccgagca tcttcgagat cctgcgtacc cgcccgatcc catcgtcctg ggcgaagaac
    10921 tttagctctt tctgacgggc gtagaacgtc gcctcgaacg ccgtccggtc catccgcagc
    10981 agatgttcct cgacccaccg cgtaacgtcc gtcgtcccgg taacgatggt cttgcccgtc
    11041 gagtcctgcg cctcggcggt cgtctggtta ctcctgagcc cccggcgcac cgtgtaggaa
    11101 tcgcccccga ccgccaacgt gacctccacc accgtcttgt ccttcgccga gcccccgctc
    11161 caggggatca actcgttcgc aaagcgcggc ccgccgcccc gcgacccgaa aaaagcccac
    11221 aatatgctct cgaacagtgt gctcttcccc gagccgttcc tgccgaccac cccgatcgcg
    11281 ccttccggcg gcagcagttc caccggctcc cgaagctgct tgtagttctc tatgtacagc
    11341 cgctccaggt tcaagaagcc tgctcctcgc tcgccgcccg cgccagatac ccgcgtccct
    11401 tctccaggaa ctcgccggcg aacttctcct ccaactcacc gcgctcccgc cgaaccttca
    11461 cgaactctgc gaactcctcc tccagcgccc cgaagaccgc cgagaccggg ccgctcgccc
    11521 gctcctccgg caactcttcg gcgtggacct ccaggctgaa attaagacag cggcgttgca
    11581 ggtcgcgaag cagcgcccgg tccaccccgc tcgtcacccc gcgccgaaca tcgaaagccc
    11641 gtaggcggac tatcgcgccg tcgagatcta caccagccgt tcgctccctg acggcatctg
    11701 tcagcgccgc ggaatccatc tctctggcct tgatcttctc caggtccagc atcggacgcg
    11761 cctctatccc aacgtgctcc acgctcttga gcccgctccc gtcgaactcc acgatggcga
    11821 accccggctt ggatgctacc tccccgaacc cgaaccgttc cgtcgccccg gcgtagaacg
    11881 cgtttggctt gtgctcgtgg aactcgtgat agtggccgag ggccacgtag tcgaaggggg
    11941 cggagatcat ggccgattgc aggtccgcct cgcccatctc gtgctgcttc gcctccagcc
    12001 ccggcaccat cccgtgcgtc acaagaatgt ttacgtccgc ctcccgcgcc ggcatgaccg
    12061 cccccgttcc gaagaccgcc ccgtgtggca ccagcgtaac gcccacgccc acgccgtctg
    12121 cctcgaccat ctcgtagtcg aggtcgaatc cgtgcgccga gtagacgt
//
LOCUS       DASSMB010000166        14962 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1812974, whole genome shotgun sequence.
ACCESSION   DASSMB010000166 DASSMB010000000
VERSION     DASSMB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14962
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1812974"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VFJ72_09850"
     CDS             complement(<1..427)
                     /locus_tag="VFJ72_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479800.1"
                     /translation="MDGRTEKTERRGDRLLLVYLAALLVVGLFTVGRIGVTWDEPNYF
                     GSTYSYLMWFAHIAADPSDWWNSIDHYWSPSYEAPPFFKLWAGLFAGAGALIFGTENL
                     DRIGDAYRMASYALFLAAIFASFRFMRREFGVAAAWGAAL"
     gene            534..1568
                     /locus_tag="VFJ72_09855"
     CDS             534..1568
                     /locus_tag="VFJ72_09855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7479801.1"
                     /translation="MQREVSDTRASTQPREAFDGVSVVVPVFNEEDCIARTHAEIRAA
                     LSGVPHEVIYVDDGSTDGTGRILEEIADGDPSARVIRFRRNFGKSAALSAGFARVGYG
                     AVATMDADMQDDPNEIPKLINQMYDEDLDLISGWKKNRKDPLEKRLPSKLFNRTTQIL
                     TGVKLHDFNCGLKVYRREVVREVTLYGELHRYIPALAHYRGFRVGEAVVNHRPRGGGS
                     SKFGLERYIRGMFDLMSIVFLGLYQRRPLHLFGGVGMIFASLGVLISAYLTVLWFMGY
                     GIGNRPLLILGVLLIIVGVQSVSFGLIAELIARTSYAASDPPYSIAREKHAELPSPDG
                     SSDGERHTGS"
     gene            1565..2761
                     /locus_tag="VFJ72_09860"
     CDS             1565..2761
                     /locus_tag="VFJ72_09860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7479802.1"
                     /translation="MKVLFVTSTFARSDEDDQVPWLLELALVLRERGVDLEVLAPTYA
                     GLASHTVHGIPVHRYRYFTKKREVVTHEAGAMNNMVGRRDLLIPALSLVGAGMAAAAR
                     LARRNHYDVIHSHWPLPMGLPAQAGAWASKKRPHLVATFHGAEVALAKRKPHYAPVLR
                     RITRNLDAAIANSNSTASKVRELTGVEATVIPFGPPRGAVDLDRSSGSKAGRGDGLPV
                     VLAVGRVIERKGFPVLVRAAERLRGRARVVIVGGGEYEPVVRREIEKTTTGDVVTLAG
                     RLSNAALADLYRSCAVFCLPAIVDSRGETEGLGVVLIEAMSHGKPVVASRLGGIVDAV
                     EDGQTGLLIPPNDPEALASALLRVIEDGELARRLGEAGRERAKKLFSWESVAERHLRV
                     YSAAAG"
     gene            2758..3702
                     /locus_tag="VFJ72_09865"
     CDS             2758..3702
                     /locus_tag="VFJ72_09865"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HET7479803.1"
                     /translation="MSRATGGPLRRVRRFVGPAVVLLAVVFLARALIGGWSEAREYEW
                     RFDPGHLGLSIVLIFLYYAQQWGGWRLIMGSFGDPLGRLESAAIWFASILGRYVPGNV
                     VMVAGRISLCRRRGIPAATTFASLVYENALILISALIVAAASVPLWPLFPYRGYALVL
                     AALAPVGLLCLHPAVFGRLANAVLRRFKQEPLGATLPFGRVLFLLLYYAGGWVLLGLA
                     FASLASAVAPVNAGDLALLVGGYAFAWEVGFLSFVTPSGLGVKEGVLLLILNLVFPAP
                     VSAALVVLSRLWQTLAELAAAAAVWTLARGRGFLAARG"
     gene            complement(3724..4446)
                     /locus_tag="VFJ72_09870"
     CDS             complement(3724..4446)
                     /locus_tag="VFJ72_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7479804.1"
                     /translation="MRHSTGGFAATSAPEGFDLADFEEAGLRAVERRFGPKDTIFTPG
                     DPDDQLYFVLSGTVRLYKIYGDYKEATTALLRDGGIFGKLNLVEGRWQDVFAEAVTEA
                     RVASIQKSAIERAMKSRPDFALRLFSSLSERLRQSDEVIESLLHREVSTRLATLLMNL
                     SERFAEENSGGIVIDVRLTHQDLANMIASTREAVSKVMSEFQRDGIIETRSRKITILN
                     RQSLAEHASGPSGLTVDGQLSI"
     gene            complement(4610..5230)
                     /locus_tag="VFJ72_09875"
     CDS             complement(4610..5230)
                     /locus_tag="VFJ72_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479805.1"
                     /translation="MEGIGKITKRKRKLRSVGEPHPWEPRHMKLAGLRCAESVGEPAA
                     RRNVTLGVVQHLWRSPVPFLGFYPPEGVKVTENRIYTPAPPRLVDDTEYLAGVEAHKR
                     AFARDLMDSMLPAGNLPGYFEVPTDEGWRVAVDLIGRTLTLADAWGLETSAAVAGDLA
                     PFVWAYRHCGFCIVTIYDRDIDALRDDLLFIAKRDGVDLRWGEKAA"
     gene            complement(5338..6027)
                     /locus_tag="VFJ72_09880"
     CDS             complement(5338..6027)
                     /locus_tag="VFJ72_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET7479806.1"
                     /translation="MGGYFGYFVIMIVGALISGAAALWVRSSYSKFSKQMSASGLTGA
                     QTARMILDRNGLTNVKVEPVAGQLTDHYDPSSKVVRLSEGNFSKNSIAAVSVAAHEVG
                     HAIQDSTGYVPMKLRAGLFPIVSFAGQLWFPLFFMGIILGASAGLGQLMIQLAIIAFA
                     GVLAFHVVTLPVEINASTRAYGLLQRYGILSATEAGGTRRVLTAAAFTYIAAALTSLL
                     TLLYLLAVSRN"
     gene            complement(6176..8110)
                     /gene="acs"
                     /locus_tag="VFJ72_09885"
     CDS             complement(6176..8110)
                     /gene="acs"
                     /locus_tag="VFJ72_09885"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HET7479807.1"
                     /translation="MTEEQTEVRSYEPPEDFVSQANINDASVYEKASEDYEGFWAEQA
                     RELHWFKEFDQVLDWDPPEAQWFVGGKINAAYNCLDYQVEQGKGDKTAFIWEGDEPGD
                     TKTYTYSELTAEVKKFANVLKDLGVQKGDRVGIYLPMIPELAIAMLACARIGAPHSVV
                     FSAFSPGSLRDRLNDAEAKVLVTADAAPRGGKVSPLKENADKALDDSPSVENVVVVKR
                     TGDDVPMTDGRDQYWQDLMEGASDDCPAEEMDSEDMLYILYSSGSTGKPKGIVHTTAG
                     YLTHVKVTNKWVHDLKDDDVYWCTADVGWVTGHSYLVYGPLANGGTTLMFEGTPAYPE
                     NDRWFDVIEKHKVTVLYTAPTAIRAFMKTGTGPLEKHDLSSLRLLGTVGEPINPRAWE
                     WYHDNVGGGRCPIVDTWWQTETGGIMITPLPGLTTTKPGSATFPFPGIEAGLYDEEGN
                     EIEGEGQGNLVIKKPWPGMLRTLYGDPQRYRETYWEKYGDTYFAGDGARRDADGYYTI
                     TGRVDDVMNVAGHRVSSAEVESALVSHKAVAEAAVVGKKDEDKGQAIFAYVILEGDRE
                     TSEELMQELRKHVRTELGPTSQPHQVVAVDELPKTRSGKIMRRILRGIAEGEQDLGDT
                     STLADPSVVDTLQEQAAAQT"
     gene            complement(8306..8860)
                     /locus_tag="VFJ72_09890"
     CDS             complement(8306..8860)
                     /locus_tag="VFJ72_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479808.1"
                     /translation="MSEWQTLTALIAGLSRQDRMLLSRFKALELPEDPDAAAEALRVH
                     ERLSPGATPSMLLATAGSQAALAGEEAFARRIGEAALDLAEDSEERRISHVCLAQIYF
                     RHRREEAALEEFVAHCRAAVDLGHTGTFCYERHAALYEYRGDLDGAIEVCSRAVEALS
                     GAGDARSAGRFRRRIDRLSAKRGG"
     gene            8977..10911
                     /locus_tag="VFJ72_09895"
     CDS             8977..10911
                     /locus_tag="VFJ72_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7479809.1"
                     /translation="MLGLLGREDWIYLLSLLVPLCLYDVALKVIRILTQLDVPGPVGF
                     LDQVRSDIFFNLGYAVLWIGLFAIIRSGIPRYLLLILFHVSALLVAVLATSAHFFYEI
                     TGSALSFDFIVLSLSKFGEVQGAITSETTPLLWVLLSVVLFYAISGPAIVTFYMTRDW
                     HVPVRTAGRPWAAPLVICVSAAAFGGLSLLPSATGASDTFSRDSLANLFVMEMSAPDM
                     KAIRAETTTELASEKQPLHTKFVSTPQTKKPNVVMIFLESTRAESTTPYKKDLKTTPF
                     LDALSKKSTVAERAYAVVPHTSKALLATSCGVAPPLDTENTESEPGVIPGRCIPQLLE
                     DHGYKTAFFQSATEDFERRRALADNFGYDEFFPVNIMDKEGFEPVNYFGYEDNIMLKP
                     SEQWLEKNGNQGPFLATYLTVTTHHNYTVPSRYPKQHFADDKVLNNYLNDVYYEDQFL
                     KALFQQYKDLGLYDNTVFILLGDHGEGFGEHGLYQHDNTIYNEGLHIPFIIHDGRDPK
                     PGRIEAPVDEMDVLPTLADILGYDIKGGTYPGSSMFSVPKNRTLMASCYHENTCLASI
                     KGDEKYIYHFGQRDDEFFDLSKDPHEQDNLDEDHKKEMEKRRHDLLAWEAKVKAIYDQ
                     RLADLSRERTSPTEKAAEDN"
     gene            complement(10921..12465)
                     /locus_tag="VFJ72_09900"
     CDS             complement(10921..12465)
                     /locus_tag="VFJ72_09900"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HET7479810.1"
                     /translation="MKHGFNGKSEPSRGAAGIALGAGAALLSFFLYIRTLAPTVLYYD
                     PRGLYDSVMLQVQAYLLDIPNPTGYPVYVLLAKLFTYLPIGDVGYRVNLASAVFGAIA
                     VFLLYLLCYRLTGRAAPAAGAALLFGVSRAFWSQAVITEVYTLNALFICLVLLVLYVW
                     RDRRKDRYLLLAAFLMGLSLSHHMTSGLLIPAAGLFVGLTEPRKLLQWRLILRGAGLF
                     LLGLTPYLYLPIRARMDPPLNESDPSTWHNFWILVSGSRFDERMFAYGLDELPGRLKL
                     FEGLLANQFHPAFLAVAALGVLYLLARDRTSLVTFMFLGLGWFVYAIEYNIKDFYIYF
                     IPVYLMLSVFVSAGFWAVVDAVDLAAQRLSSKRRAAILAVLAVPILLSPLSRVDESYK
                     AVDRSHDYTGRNMIEAVAGGTAKDSTVLHNGSSLWYMTLVEKRRTDLRLVDPFEPGDW
                     TAKTRKWVAESSRFLKDGHVYILFPGTTEALNRPLFREDGYDLIPRHGGIFYEVVVRD
                     RRREPR"
     gene            12595..13374
                     /gene="fabI"
                     /locus_tag="VFJ72_09905"
     CDS             12595..13374
                     /gene="fabI"
                     /locus_tag="VFJ72_09905"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HET7479811.1"
                     /translation="MGGLLEGKKILVTGVLDRRSIAYSAARLAQEQGAEIVLSGFGRA
                     RSLTERTAKRLDPEPPVLELDANSPDDIAAVADDLKERWDTLDGIVHAIAFAPEDALG
                     GNFLTTEWPSVATAVQTSAYSLKALSVGLADLMKPGSSVVGLDFDARIAWPAYDWMGV
                     AKAALESTSRYLARDLGERGIRVNLISAGPVKTLAAKGISGFESIEGEWGRQAPLGWE
                     TTNPEPVGKAIVSLLSDWWPATTGELIHVDGGYHAMGAPIK"
     gene            complement(13393..13965)
                     /locus_tag="VFJ72_09910"
     CDS             complement(13393..13965)
                     /locus_tag="VFJ72_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479812.1"
                     /translation="MRRATILITLALLMLAVAGVSSAREGAFVGLLGDEEIASEPTEA
                     TQPLVSGTTTHEATVIEETTVTAHPETAVPDDADEPDESVEDPGRPNSDAGKIEREPG
                     KAPASEDKKIGKPTAGEKPAGKGESAERGLSHRQDNARPDKARGKAETGKAGGQPKAT
                     VCHRGKTLTIGAPAKPAHLRHGDSLGACAG"
     gene            complement(14053..>14962)
                     /gene="dnaB"
                     /locus_tag="VFJ72_09915"
     CDS             complement(14053..>14962)
                     /gene="dnaB"
                     /locus_tag="VFJ72_09915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7479813.1"
                     /translation="IYSVSNRSLGEQLSPVSELAPGALEMIQKLYEQEGEVTGVETGF
                     DDLDKLTTGFHKSDLVVLAARPAMGKTAMALNAIWHAAGVKKMPVAIFSLEMSKEQLV
                     QRLISQTTRIPTQDLRSGNVKAEDWPKLVRGVGEVSGAPIWIDDTAGASLMEMRAKVR
                     RLASRLIAQGGPPLSLVVVDYLQLMIGNNRENRQQEIAEISRGLKVLARDLDVPVLAI
                     AQLSRAVEQRHDKRPLLSDLRDSGAIEQDADMVMFLYRDEYYNPDSDDKGIAEVIVGK
                     HRNGPTGKVQLAWLEQYTKFASLARR"
BASE COUNT         2537 a         4925 c         4802 g         2698 t
ORIGIN      
        1 aaagagcagc tccccacgcc gccgcaactc cgaactcccg gcgcatgaag cggaaggacg
       61 cgaatatggc ggcgaggaag agcgcgtagg aggccatccg gtaggcgtcg ccgatgcggt
      121 cgaggttctc cgtcccgaag attagcgccc cggctccggc gaagagcccg gcccacagct
      181 tgaagaacgg cggcgcctcg tagctcgggc tccagtagtg gtctatggag ttccaccagt
      241 cggaggggtc tgcggcgatg tgcgcgaacc acatgaggta cgagtaggtg gagccgaagt
      301 agttaggctc gtcccacgtc accccgatcc tcccgacggt gaacagcccg acgacgagca
      361 gcgccgcgag gtagaccagt aagagcctgt cccctctcct ctcggtcttc tccgttcgcc
      421 cgtccacggg cctattctag ccgcggcgat gcgcggcggc cgtccggcgg cgggagttaa
      481 ctttaggtta aagcgcttcg gggagcgaaa aggctgcgct atactgtccg actatgcagc
      541 gcgaagtctc ggatacacga gcctcaacgc agccgcgtga ggctttcgac ggcgtctcgg
      601 tggtggtgcc ggtcttcaac gaggaggact gcatcgcgcg gacgcacgcg gagatccggg
      661 cggcgctctc cggcgtcccg cacgaggtca tctacgtgga cgacggttcg acggacggga
      721 cgggcaggat actcgaagag atagcggacg gcgacccctc ggcgcgcgtg atccgcttcc
      781 ggcgcaactt cggcaagagc gcggccctca gcgccgggtt cgcccgcgtg ggctatggcg
      841 cggtagccac tatggacgcc gacatgcagg acgacccgaa tgagatcccg aagttaatca
      901 accagatgta cgatgaggac ctggacctga tctccggctg gaagaagaac cgcaaggacc
      961 cgctggagaa gcgtctccct tccaagctct tcaacaggac cacccagatc ctaaccggcg
     1021 taaagctcca cgatttcaac tgcggcctga aggtctaccg gcgcgaggtg gtgcgggagg
     1081 tgacgctcta cggcgagctc caccgctaca taccggcgct cgcacattac cggggcttcc
     1141 gggtcgggga ggcggtagtg aaccaccgtc cgaggggcgg cggctcgtcg aagttcggcc
     1201 tggaacgcta catccgcggg atgttcgacc tcatgagcat cgtcttcctg gggctgtacc
     1261 agagacggcc gctgcacctg ttcggcggcg tcgggatgat cttcgcctcc ctgggcgtgc
     1321 tcataagcgc gtatctgacg gtgctgtggt tcatgggata cggcatcggg aaccggccgc
     1381 tactcattct cggcgtgctg ctcatcatag tcggcgtaca gagcgtctct ttcggcctga
     1441 tcgccgagct gatagcccgc acgagctacg ccgcgagcga cccgccatac agcatcgccc
     1501 gcgagaaaca cgcggagctt ccctcgccag acggctcgtc cgacggcgaa cgtcacaccg
     1561 gcagttgaag gtcctcttcg taacctcgac cttcgcgcgc tccgacgaag acgatcaagt
     1621 cccctggctc ctggagctcg ccctcgtcct gcgcgaacgc ggcgtcgacc tcgaagtcct
     1681 cgcccccacc tacgccggtc tcgcaagcca cacggtccac ggcatccccg tccaccgcta
     1741 ccgctacttc acgaagaagc gcgaggtcgt cacccacgag gccggcgcga tgaacaacat
     1801 ggtcggacgc agggatctcc ttatcccggc cctctcgctc gtcggggcgg ggatggccgc
     1861 tgcggcgcgg ctcgcccgcc gaaaccacta cgacgtgatc cactcgcact ggcccctccc
     1921 gatggggctc cccgcccagg cgggggcatg ggcgtcgaag aagagacccc acctcgtggc
     1981 taccttccac ggcgcggagg tcgcgctggc gaaacgcaaa ccccactacg cgccggttct
     2041 gcgccgtata acgcgcaacc tggacgcggc catcgccaac tccaacagca ccgcgtctaa
     2101 ggtaagggaa ctgactggag tagaggcgac agtcatcccg ttcgggccgc cccgtggcgc
     2161 ggtggacctc gatagatcgt ccggatccaa ggctggccgc ggcgacgggc tccccgtagt
     2221 gctggcggtc gggcgcgtga tcgagcgtaa gggcttcccg gtgctggtgc gggcggcgga
     2281 gcggctgcga ggtcgggcgc gggtcgtgat cgtgggcggc ggcgagtacg agccggtggt
     2341 gcgccgggag atagagaaga ccacgaccgg cgacgtggtg acgctcgcgg gccggctatc
     2401 taacgcggcg ctcgccgacc tgtatcgaag ttgcgcggtc ttctgcctgc cggccatcgt
     2461 ggactcccgc ggcgagaccg aggggttggg ggtcgtcctg atcgaagcca tgagccacgg
     2521 caagcccgtc gtcgccagcc gcctcggcgg tatcgtggac gcggtcgagg acggtcagac
     2581 cgggcttctg atccccccga acgacccgga ggcgctggcg agcgcactgc tgcgcgtgat
     2641 cgaggacgga gagcttgcgc gccgtctggg agaggcggga cgggagcggg ctaagaagct
     2701 cttctcgtgg gagagtgtgg cggagcggca cctgcgggtc tactcggcgg cggcagggtg
     2761 agcagagcga ccggcggtcc gctgcgccgg gtgcggcggt tcgtcgggcc ggccgtggtc
     2821 ctgcttgcgg tggtgtttct cgcgcgggcg ctcatcgggg gctggagtga ggcgcgggag
     2881 tacgagtggc gtttcgatcc cggacacctc gggttgtcta tcgtcttgat cttcctctac
     2941 tacgctcagc agtggggcgg gtggcgcctg atcatgggca gcttcggcga tcctttgggg
     3001 cggctggagt cggcggcgat ctggttcgcc tccatcctcg gacggtacgt gccgggcaac
     3061 gtggtgatgg tcgccggacg catctcgctg tgccgccggc gcggaatacc ggccgccacg
     3121 acgttcgcga gcctcgtcta cgagaacgcc ttgatcttga tctccgccct catcgtcgcc
     3181 gcagcctcgg tgccgctctg gccgcttttc ccgtaccgcg gctacgctct tgttctcgcc
     3241 gccctcgcgc ccgtcggcct cctctgcctc caccccgccg tcttcgggcg tctggcgaac
     3301 gccgtgctac gccggttcaa acaggagccg ctgggggcga cgctgccgtt cgggcgggtg
     3361 ttgttcctgc tgctctacta cgccggtgga tgggtgctgc tcgggctcgc cttcgcctct
     3421 cttgcgtccg ccgtggcgcc ggtaaacgct ggagacctag cgttgctcgt cgggggttac
     3481 gccttcgcgt gggaggtagg gtttctatct ttcgttacgc cgagcggtct cggagtcaag
     3541 gaaggggtgc tgctcctgat cctgaacctc gtctttccgg ctccggtctc cgccgcgctg
     3601 gtcgtcctct ccaggctctg gcagacgctg gcagagcttg cggccgccgc ggccgtctgg
     3661 acgctggctc gcgggcgcgg atttctggcg gcccgcggct gaggagcggc gctcgtagag
     3721 ccgctagatc gagagttgcc cgtccacggt caggccggag ggtccggagg cgtgctccgc
     3781 gagggactgt cggttcaaga tcgtaatctt tcggctgcgc gtctcgatga tgccgtcgcg
     3841 ctggaactca ctcatcacct tcgagacggc ctcgcgggtc gaggcgatca tgttagcgag
     3901 gtcctggtgc gtgagccgca cgtcgatgac gatgccgccg ctgttctcct cggcgaagcg
     3961 ctcgctcagg ttcatgagca aggtcgccag ccgcgtggag acctcgcggt gcagcaggct
     4021 ctctatgacc tcgtcggact ggcgcagccg ctccgagagc gaggagaaga gccggagcgc
     4081 gaagtccggg cggctcttca tggcgcgctc gatggcggac ttctggatgc tcgcgacgcg
     4141 ggcttcggtg acggcctcgg cgaagacgtc ctgccagcgg ccctcgacca ggttgagctt
     4201 tccgaagatg ccgccgtccc gcagaagcgc ggtcgtggcc tccttgtagt cgccgtagat
     4261 cttgtacagc cgcaccgtgc cggagagtac gaagtagagt tggtcatcag gatcgccggg
     4321 ggtaaagatg gtgtccttcg gaccaaaccg gcgctctacg gcgcgcagcc ccgcctcctc
     4381 gaagtccgcg agatcgaagc cctccggtgc gctggtagcg gcgaagcctc ccgtactatg
     4441 cctcatgagc atctgtgtat tgcccgcctc tctgctagaa acatcccctg ccgcccgacc
     4501 gccggtatcc cccgaacgcc gggatgcctc catcggtagg ctcacattat acccgaccgc
     4561 gaacggctac tgcgcctggc taccgcctag cgatcaactg attaacgcgt caggccgcct
     4621 tctcgcccca cctcaggtct acgccgtcgc gcttggcgat gaagagcaga tcgtcccgca
     4681 gggcgtctat gtcccggtcg tagatcgtaa cgatgcagaa cccgcagtgc cggtatgccc
     4741 acacgaacgg cgccaggtct ccggcgaccg ccgcgctcgt ttcgagcccc caggcgtccg
     4801 cgagggtgag agtgcgcccg atcaggtcca ctgcgacgcg ccatccctcg tcggtaggca
     4861 cctcgaagta gccggggagg ttgcccgccg gcagcatgct gtccatgagg tctcttgcga
     4921 aagcccgctt gtgcgcctcg accccggcga ggtactccgt atcgtcaacg agcctcggtg
     4981 gcgcgggagt gtagatccgg ttctccgtga ccttcacgcc ttccggcgga tagaaaccga
     5041 ggaacggcac cggcgagcgc cacagatgct ggaccacgcc gagggtcacg ttgcgccggg
     5101 cggcaggctc cccaaccgac tccgcgcagc gcagcccggc gagcttcatg tgccgcggtt
     5161 cccacggatg tggttccccc acgctccgca gcttccgctt cctcttggtt atctttccga
     5221 taccttccac ggcacgagca ttttagcacc gaacctgcgc ccgttgcccc cctttacgat
     5281 gcacgaaaaa ggacggggca tgatgccccg cccggtactg taagcaaaga cgttcctcta
     5341 attgcggctg acagcgagca ggtagagcag tgtcaggagc gaggtgagcg cggccgctat
     5401 gtaggtgaag gccgccgccg tcaagacgcg ccgggtgccg ccggcctccg tcgccgagag
     5461 gatgccgtaa cgctggagca gcccgtaggc gcgggtggag gcgttgatct ctaccggcag
     5521 tgtcaccacg tggaacgcca gcaccccggc gaaggcgatg atcgctagtt ggatcatcaa
     5581 ctgcccgagc ccggcgctcg cgcccaggat gatccccatg aagaacagcg ggaaccagag
     5641 ctgcccggca aagctgacta tcgggaagag ccccgcccgg agcttcatcg gcacgtatcc
     5701 ggtcgagtcc tggatcgcgt gcccgacctc gtgcgcggcc acgctgaccg cggcgatgga
     5761 gttcttcgag aagttgcctt ccgagagccg cacgacctta ctcgacgggt cgtaatggtc
     5821 ggtgagttgt ccggccaccg gctccacctt cacgttcgtc aggccgttcc gatccaggat
     5881 catgcgcgcc gtctgcgccc cggtaaggcc gctggcgctc atctgtttgg aaaacttcga
     5941 gtaggagctt cgcacccaga gagccgcggc gccggagatg agagcgccca cgatcattat
     6001 cacgaagtat ccgaagtaac cgcccaacag ttgttcctcc ttaaggatgc cgcttaacgg
     6061 acatatttta ctacacacct atacgcgcca accctcaaaa agtctcacgt ttacgggcaa
     6121 accaaagggg acggccgcac gaccgtcccc tgttttcgac agttttctac ttgtatcagg
     6181 tctgggcggc ggcctgctcc tggagcgtgt ccacgaccga aggatcggcg agggtcgagg
     6241 tgtcgccgag atcctgctcg ccctcggcga taccgcgcag tattcggcgc atgatcttgc
     6301 cgctgcgcgt ctttggcagc tcgtccacgg cgacgacctg gtgcggctgc gacgtggggc
     6361 caagctcggt gcgcacgtgc ttgcgcagct cctgcatgag ctcttcgctg gtctcccggt
     6421 cgccttcgag gatgacgtag gcgaagatcg cctgcccctt gtcctcgtct ttcttgccta
     6481 ctaccgccgc ctctgccacg gccttgtgag agactagcgc gctctcgacc tccgccgagc
     6541 ttacgcggtg tccggcgacg ttcatcacgt cgtccacgcg gccggtgatg gtgtagtagc
     6601 cgtccgcgtc gcgccgggcc ccgtcgcccg cgaagtaggt gtcgccgtac ttctcccagt
     6661 aggtctcccg gtagcgctgc gggtcgccgt agagcgtgcg gagcatgccg ggccagggct
     6721 tctttatgac caggttgccc tgcccctcgc cctcgatctc gttcccttcc tcatcataga
     6781 ggccggcctc gatgccgggg aacgggaagg tcgcgctgcc gggcttggtg gtggtgaggc
     6841 cgggcagagg tgtgatcatg atgccgcccg tctccgtctg ccaccaggta tctactatcg
     6901 ggcagcgccc gccaccgacg ttgtcgtggt accactccca ggccctgggg ttgatcggct
     6961 ctcccaccgt cccgagcagc cgcaggctgg agaggtcgtg cttctccaac ggcccggtcc
     7021 cggtcttcat gaacgcccgt atcgcggtcg gcgcggtgta gagcaccgtc accttgtgct
     7081 tctcgatgac gtcgaaccag cggtcgttct ccgggtacgc cggcgtcccc tcgaacatga
     7141 gggtggtccc gccgttggcg agcggcccgt agacgaggta tgagtgcccc gtgacccagc
     7201 caacgtccgc cgtgcaccag tacacgtcgt cgtccttgag gtcgtggacc cacttgttcg
     7261 tgaccttgac gtgcgtgagg tagccggcgg tggtgtggac gatgcccttc ggtttgccgg
     7321 tcgagccgga ggagtagagg atgtagagca tgtcctccga gtccatctcc tcggccgggc
     7381 agtcgtcgct cgcgccctcc atcaggtcct gccagtactg gtcgcggccg tcggtcatcg
     7441 gcacgtcgtc gcccgtgcgc ttcacgacca ctacgttctc gacgctcggc gagtcgtcga
     7501 gagccttatc agcgttctcc ttgagcgggg agaccttgcc gccgcgcggg gcggcgtcgg
     7561 cggtaaccag tacctttgcc tcggcgtcgt tgaggcggtc gcgcagggag ccgggcgaga
     7621 aggcgctgaa gacgaccgag tgcggagcgc cgatgcgggc gcaggccagc atcgctatgg
     7681 cgagctccgg gatcatgggc aggtagatgc cgaccctgtc gcccttctgt accccgaggt
     7741 ccttgaggac gttggcgaac ttcttgacct ccgccgtcag ctcggagtag gtgtaggtct
     7801 tcgtgtctcc gggctcgtcg ccctcccaga taaaggcggt cttatcgccc ttgccctgct
     7861 cgacttgata gtcgaggcag ttataggcgg cgttgatctt gccgcccacg aaccactgag
     7921 cctcgggagg gtcccagtcg aggacctgat cgaactcctt gaaccagtga agctcgcggg
     7981 cctgctcggc ccagaagcct tcgtaatctt cggaagcctt ctcatagacc gaagcatcat
     8041 tgatgttcgc ctgcgagacg aaatcctccg gcggctcgta actgcgtact tctgtctgct
     8101 cttctgtcat atattgctcc tttccctgtt tcaacctcga accgggccat ccatcatgtt
     8161 tcccacgtca accgcgtacc aaacgaacgg cgccgcatgg ttcgatcccc gatccccaaa
     8221 ccaggacccc tatatcccct ccgtgcttcc ccctttcccg gccactcgta acgcgcctcc
     8281 aagataattc ggtagcagta tacgatcaac cgccgcgctt cgcggagagc cggtcgatcc
     8341 gtctccggaa ccgcccggcg gaacgcgcgt ctccggcccc ggagagtgct tcgacggccc
     8401 ggctgcatac ctcgatggcg ccgtcgaggt cgccccggta ctcgtagagc gccgcatgcc
     8461 tctcgtagca gaaggtcccc gtgtgcccga ggtccacggc cgcccggcag tgcgccacga
     8521 actcctccag cgccgcctcc tcgcggcggt gccggaagta aatctgcgcg aggcatacgt
     8581 gggagatccg gcgctcctcg gagtcttcgg cgaggtctag cgcggcttcg ccgatgcgcc
     8641 gggcgaatgc ctcctctccg gccagagcag cctgactccc ggccgtggcg agcagcatcg
     8701 agggagtagc tcccggactc agccgctcgt ggacgcgaag cgcctcggcc gcggcgtcgg
     8761 ggtcctctgg cagttccagg gctttgaacc ggctcaggag catgcgatcc tgacgtgaca
     8821 agcccgcgat cagtgctgtc agagtctgcc attcggacac gacttctcct ctcaccgcag
     8881 ccctatctct accgcaaatc ctcatattat gaaatatttg ttaaactccc cgcattcatg
     8941 agcggcagga gtaacttcag gggtatgcgg gcccgcgtgc tgggcctgtt gggccgggag
     9001 gattggatct accttctgag tctcctcgtg ccgctgtgtc tctacgacgt cgccttgaaa
     9061 gttatccgga tattgaccca gctcgacgtg ccggggcccg tcggtttcct ggaccaggtg
     9121 cgctccgaca tcttcttcaa cctgggttac gcggtactct ggatcgggct cttcgccatc
     9181 atccggtccg gcatcccccg ctacctcctt ctcatcctgt tccatgtctc ggccctgctg
     9241 gtcgccgtac tcgccacgag cgcgcacttc ttctacgaga tcaccggctc cgccctgagc
     9301 ttcgacttca tcgtgctctc gctctcgaag ttcggagagg tgcagggcgc gataacctcg
     9361 gagaccaccc cgctactctg ggtccttctc tcggtcgttc tgttctatgc gatttccggt
     9421 cccgccatcg tgacgttcta catgacccgc gactggcacg tccccgtccg gaccgccggc
     9481 agaccgtggg cggcgccgct cgtcatctgc gtctcggcgg ccgcgttcgg cgggctctcg
     9541 ctgctgccga gcgcgacggg cgccagcgac accttctcca gggactccct ggcgaacctc
     9601 ttcgtcatgg agatgtccgc cccggacatg aaagcaatcc gggccgagac gaccaccgag
     9661 ctggcctcgg agaagcagcc gttgcacacg aagttcgtca gcacccctca gacgaagaaa
     9721 ccaaacgtgg tgatgatctt cctggagtcc acccgcgccg agtccaccac cccctacaag
     9781 aaagacttga agaccacacc gttcctcgac gcgctctcca agaagagtac cgtggcggag
     9841 cgggcctacg cggtagtccc ccacacctcg aaggcgctgc tcgcaaccag ttgcggcgtc
     9901 gcgccgccgc tcgataccga gaacaccgag tccgagccgg gcgttatccc cggccgctgc
     9961 atcccccagc tcctggaaga ccacggctac aagacggcct tcttccagtc ggcgaccgag
    10021 gacttcgagc gccgccgggc gctggcggac aacttcggtt acgacgagtt cttccccgtc
    10081 aacatcatgg acaaggaggg cttcgagccg gtcaactact tcggctacga ggacaacatc
    10141 atgctgaagc cgagcgagca atggctcgaa aagaacggca accaggggcc gttcctggcg
    10201 acctacctca cggtcaccac ccatcacaac tacacggtcc ccagccgcta ccccaagcag
    10261 cacttcgcgg atgataaggt gctcaacaac tacctgaacg acgtctacta cgaagaccag
    10321 ttcctgaagg ccctcttcca gcagtacaag gacctcgggc tctacgacaa caccgtcttc
    10381 atcttgctcg gcgaccacgg cgagggtttc ggggagcacg gcctctacca gcacgacaac
    10441 accatctaca acgaggggct tcacatacca ttcattattc atgatggccg cgacccgaag
    10501 cccggacgca tagaagcccc ggtagacgag atggatgtcc tcccgacgct cgccgatatc
    10561 ctcggctacg acatcaaggg cggtacgtat ccgggctcat cgatgttctc ggtgccgaag
    10621 aaccgcacgc tgatggcgag ctgctaccac gagaacacct gcctcgccag catcaagggc
    10681 gacgagaagt acatctacca cttcggacag cgggacgatg agttcttcga cctgtcgaaa
    10741 gacccgcacg agcaagacaa cctggatgag gatcacaaga aggagatgga gaaacgccgc
    10801 cacgacctgc tggcctggga ggcgaaagtc aaggcgatct acgaccagcg cctcgccgat
    10861 ctcagccgcg aacgcacctc gccgacggag aaggccgccg aagacaacta gcggctctct
    10921 ctagcgcggc tctcttcgcc ggtcccgcac gacgacctcg tagaagatcc ccccgtgtct
    10981 ggggatgaga tcgtacccgt cctccctgaa cagtggccgg ttcaacgcct cggtggtgcc
    11041 ggggaagagt atgtagacgt ggccgtcttt caggaatcgg ctcgactcgg cgacccattt
    11101 cctggtcttg gccgtccagt cgccgggttc gaacgggtcc acgagccgca ggtccgtgcg
    11161 gcgcttctct accagggtca tgtaccagag cgagctgccg ttgtgcagga cggtcgagtc
    11221 cttcgccgtg ccacccgcga cggcttctat catgttgcgc ccggtgtagt cgtggctacg
    11281 gtcaaccgcc ttgtacgact cgtcaaccct ggagagcggg gagaggagga tcggcaccgc
    11341 gaggacggcc agtatcgcgg cgcggcgctt cgaggagaga cgctgcgccg cgaggtccac
    11401 ggcgtccact acggcccaga agcccgcgct cacgaacacg gacagcataa ggtacacggg
    11461 gatgaagtag atgtagaagt ccttgatgtt gtactcgatg gcgtacacga accatcccaa
    11521 acccaggaac atgaatgtga cgaggctggt ccggtcgcgc gcgagcaggt acaggacgcc
    11581 gagcgccgcg accgccagga atgcgggatg gaactggttg gcgagcaaac cctcgaacag
    11641 cttgaggcgt ccggggagtt catccagtcc gtaagcgaac atccgctcgt cgaaccggct
    11701 gccggagacc agtatccaga agttgtgcca ggtggacggg tcgctctcgt tgagcggcgg
    11761 gtccatgcgc gcccgtatgg gcaggtacag gtaaggcgtg agtccgagca ggaagagccc
    11821 ggcaccgcgc aggatcaggc gccactgaag cagctttctc ggctcggtca ggccgacgaa
    11881 gagccccgcc gcagggatta gcagaccgct cgtcatgtgg tggctgaggg agagccccat
    11941 caggaaggcg gcgagcagca ggtagcggtc cttcctcctg tcgcgccaca cgtagaggac
    12001 gagcagcacc aggcagatga acagcgcgtt gagggtatag acctcggtga tgacggcctg
    12061 gctccagaag gcgcggctga cgccgaagag cagggccgcc ccggccgccg gcgcggccct
    12121 acccgtgagc ctgtaacaga gcaggtagag caaaaagacc gcgatagccc cgaagacggc
    12181 cgaagcgagg tttacccggt agccaacgtc cccgataggc aggtacgtga agagcttggc
    12241 gagcagcacg tacaccgggt acccggtcgg gttcgggatg tcgagcaggt acgcctgcac
    12301 ctgtagcatg accgagtcgt agagcccgcg cgggtcgtag tagagcacgg tcggggcgag
    12361 cgtcctgatg tagagaaaga aggagagcag cgcggccccg gccccgagcg ctatcccggc
    12421 cgcgccgcgg gacggctcgc tctttccgtt aaatccgtgt ttcacggtac gggattgtaa
    12481 ccacttgacc gccacgatgc ccgccagggc cgcaaactct agccagcggg agaagcttcc
    12541 cctagactac tccgtcggag agactgctat caggacagaa cgggaggacg cgcgatgggc
    12601 gggctattgg aaggtaaaaa gatactggtg acgggggtcc tcgaccgccg atccatcgcc
    12661 tactcggcgg cgcggctggc ccaggagcag ggcgcggaga tagtcctctc cgggttcggc
    12721 cgggcgagga gcctcaccga acgcaccgcc aaacgcctcg accccgaacc gcccgtcctt
    12781 gagctggacg ccaacagccc ggacgacatc gccgccgtgg cggacgacct gaaagagcgc
    12841 tgggacacgc tggacgggat cgtacatgcc atcgccttcg cgccggaaga cgccctcggc
    12901 ggaaacttcc tgacgaccga atggccctcc gtggcgacgg ccgtgcagac ctcggcttac
    12961 tccctgaagg cgctctcggt aggactcgcg gacctgatga agcccggctc ctccgtggtc
    13021 gggttggact tcgacgccag gatcgcctgg cccgcctacg actggatggg cgtggcgaag
    13081 gcggcgctgg agtccacgag ccgctacctg gcccgcgacc tgggcgagcg cggcatccgg
    13141 gtgaatctga tctccgccgg tcccgtaaag acgctcgccg cgaaagggat cagcggcttc
    13201 gagagtatag agggcgagtg gggacggcag gcgcctctgg ggtgggagac aacgaacccc
    13261 gagccggtag gaaaagccat cgtcagcctg ctctccgact ggtggccggc gacgacggga
    13321 gagttgatcc acgtggacgg cggataccac gcgatgggcg ccccgatcaa ataacgccgt
    13381 tcgtcgggca agttaaccgg cgcacgcgcc gaggctgtca ccgtgacgga gatgggctgg
    13441 cttcgcgggc gcgccgatag tcagtgtctt tcccctgtga cagacggtgg ccttcggctg
    13501 tccaccagcc ttgccggtct cggctttgcc ccgcgccttg tccggacggg cgttatcctg
    13561 ccgatgcgag agtccacgct ccgcggactc gcccttgcca gcgggcttct ctcccgctgt
    13621 tggtttcccg atcttcttat cctccgaggc cggagctttg ccgggctctc tctcgatctt
    13681 gcctgcatcg ctattgggtc ggccgggatc ttctacagac tcatccggtt cgtcggcgtc
    13741 atcaggaacc gcggtctcgg ggtgagcggt tacggtcgtc tcctcgataa ccgtcgcctc
    13801 gtgagtcgta gtcccggaga ccaggggctg ggtagcttcg gttggctcag acgcgatttc
    13861 ttcgtctccg agtaggccga cgaaggcacc ctcccgggcg gagctgacac ccgcgacggc
    13921 cagcatgaga agggctagag ttatgagtat cgtggctcga cgcatgggcg cttcctatcg
    13981 ggatccttcg ctacgaacct gttatcggca caccgccgga gaaactttag cgtccgggca
    14041 ataccgcgca ggctagcgcc gcgcgagaga ggcgaacttg gtgtactgct ccagccacgc
    14101 gagctgcacc ttgccggtcg ggccgttgcg gtgcttgccg acgatgacct cggcgatgcc
    14161 cttgtcgtcg gagtcggggt tgtagtactc gtcgcggtag aggaacatca ccatgtccgc
    14221 gtcttgctcg attgcaccgc tatcacgaag gtccgagagg agagggcgct tgtcgtggcg
    14281 ctgctcgacg gcgcgggaga gctgcgctat agcaagcacc ggcacgtcga ggtcgcgggc
    14341 gaggaccttg aggccgcgcg agatctccgc tatctcctgc tggcggttct cgcgattgtt
    14401 gccgatcatg agctgcaggt agtccaccac cacgagcgag agcggcggcc ctccctgcgc
    14461 gatgaggcga ctggcgagcc tcctgacctt cgctcgcatc tccatcagcg aagctccggc
    14521 ggtgtcgtcg atccagatgg gagccccgct gacctccccc acaccgcgca ccagcttcgg
    14581 ccagtcttcg gccttgacgt tgccgctgcg gaggtcctgc gtgggtattc gcgtggtctg
    14641 ggagatgaga cgctgcacga gctgctcctt gctcatctcc agggagaaga tggcgaccgg
    14701 catcttcttg acccccgccg cgtgccagat agcgttgagg gccatggcag tcttccccat
    14761 agcaggccgc gctgccagca ccacgaggtc gctcttgtgg aagccggtcg tgagtttatc
    14821 gaggtcgtcg aagccggtct cgacgccggt tacttcgccc tcctgctcgt agagcttctg
    14881 gatcatctcc agcgcccccg gcgcgagctc cgacaccggc gagagctgct cgccgagcga
    14941 acggttggag acgctgtaga tg
//
LOCUS       DASSMB010000167         7021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1813156, whole genome shotgun sequence.
ACCESSION   DASSMB010000167 DASSMB010000000
VERSION     DASSMB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7021
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813156"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VFJ72_09920"
     CDS             complement(<1..310)
                     /locus_tag="VFJ72_09920"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7479814.1"
                     /translation="MSQEENKAVIRRFLEEVFGGGDLELVDELFAPDYVLHDPAVPGE
                     VRGPDGIRQYVDMYRSAYPDTHFTIEDQLAEDDEVVTRWTGQGTQEGVLMGIAPTGTR
                     V"
     gene            complement(431..2521)
                     /locus_tag="VFJ72_09925"
     CDS             complement(431..2521)
                     /locus_tag="VFJ72_09925"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7479815.1"
                     /translation="MSPGLASRLNAERHRRFVGRFAERDLFRSALSADGSPFNVLYVS
                     GPGGVGKTTLLREFANICEEFGVPASYVDARNVEPSSDAFLGAISSALGPAMRDGSPG
                     SIETTTERRVVLIDTYETLAPLDSWMSEVFLPQLPENLLLVLASRDPLGTNWRTDPGW
                     QRLIHPVQLRNLSLEESQAYLNRREVPSEQHQDILEFTHGHPLALSLVADVFVQRSGV
                     RFRPSETPDVIKTLLEQFVQQVPGPAHRTALESCALVRYTTESLLGHMVTMPEVHELF
                     EWLRGLTFIESGPLGLFPHDVAREALVADLRWRNPDWYAELHHRARNYYAARLKQTHA
                     QEQQRVLFDYMYLHRDNAMIKPFLEWQETGTTLPDTMRENDRPALREMIEEHEGPESA
                     RLASRWFDRQPGGVVIFRDIEQRPAGFVMKVALQETSAEDREADPAVEVAWRYLERNA
                     PLRPGERSTLFRFWMARDTYQEVSAMQSLIFGNVAQHYLTTPHLAFTFFPTADPDFWA
                     PAFAYFDLARTPEADFEVDGHHYGTYTHDWRVMPPMAWLDLLAEREISTEPLDASHST
                     ETEPLIVLGEPEFKTAIRDALRDFHSSEALHANPLLHSRTVAERAGAGADDSVSISAL
                     RDLLKEAAESLRASPREAKCYRALYHTYLNPAPTQQLAAEVLDLPFSTYRRHLKGGIT
                     RVGEILWHKEIGGQ"
     gene            2776..2964
                     /locus_tag="VFJ72_09930"
     CDS             2776..2964
                     /locus_tag="VFJ72_09930"
                     /inference="COORDINATES: protein motif:HMM:NF019968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HET7479816.1"
                     /translation="MADTQQITWDPEADRRIKRSPFLMRRFIRKRIEDRVASRGRNHV
                     TEADVDESAGMYRQYRFK"
     gene            3064..3750
                     /locus_tag="VFJ72_09935"
     CDS             3064..3750
                     /locus_tag="VFJ72_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7479817.1"
                     /translation="MSEQSQQEARSPLVSDRGVTTISDKVVSQIAGMAAREVRGLHLG
                     GSASRTTGGLLENVTGSSSSTRGISVEVGRVEAALDLTLSVDYGTDILPALEEARRKI
                     SDRVSSMTGLRVTECNVTISDIVLPTSDGDGESQGKRRDELEGGARTGARPNARTVEL
                     ESEVGDREVGEVEPRSRTRTDLAGGPAPEEVRVEGEPVDEDETVRMDARDLRDEERGR
                     GRRRDRRRDN"
     gene            3794..4291
                     /gene="mug"
                     /locus_tag="VFJ72_09940"
     CDS             3794..4291
                     /gene="mug"
                     /locus_tag="VFJ72_09940"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HET7479818.1"
                     /translation="MPPDLLRPGLALVFCGYNPSLASGRTGRHYAHPGNRFWRVLHAA
                     GITERLYSPEEDEELLERGIGFTNLCARPTRRADELSKAEIRKGSGELLARLESLRPA
                     AVAYTGIGVYRWFRATSKVEWGVQMAPAVPEVTDVVVPSPSGLNRMRFEELVVHYRKL
                     APFVS"
     gene            complement(4524..5255)
                     /locus_tag="VFJ72_09945"
     CDS             complement(4524..5255)
                     /locus_tag="VFJ72_09945"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HET7479819.1"
                     /translation="MNRPRRRLPLLNYNTFSIAAHDPETGMYGISVSTKVPAVGAITN
                     YARAGVGAIVTQAHANPLFGLDGLNMLQQGYGAEKTIRALLDSDHEPERRQLSVIDSR
                     GRAAAYTGEDCNEWRGHYTGEGYSVAGNLLVGEATISSMAEAFEASAGEWFPERLMRA
                     LEAGQEAGGDRRGRQSAAIYVVHHEPYSYLDLRVDEHAEPVAELRRVFEVVRTDLLPF
                     VERLPTRANPEGELGEEILGSVPED"
     gene            complement(5308..>7021)
                     /locus_tag="VFJ72_09950"
     CDS             complement(5308..>7021)
                     /locus_tag="VFJ72_09950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HET7479820.1"
                     /translation="MNETMNDRGPDGGGFWSQGGVALGHRRLKIIDLSECAAQPMTDP
                     ELGLTIVFNGCIYNYPELREELIKHGYRFFSTSDTEVLLKAYHRWGDNFVDHLKGMFA
                     FCIVERDSGRAILGRDRLGIKPLYLSDTGDSLRFASTLPALLSGGGVDTSIDPVALHH
                     YMMFHAVVPAPNTILKGVRRLAPATLLTIEPDGSRKTREYWSPSFTRDPAHEGMSARD
                     WEDAVLEALRVAVERRMVADVPVGVLLSGGLDSSIIVGLLAELGQKDIQTFSVGFESV
                     GGIEGDEFKYSDLVAKHYGTDHHQLRVSSERMLPALHGAISAMSEPMVSHDVVGFYLL
                     SREVAQHVKVVQSGQGADEVFAGYDWYPPMEDAEGHGLDTYEGEFFDRPRSEIEEALN
                     PEYLAGEDVSREFAGRHFSRPGADTPLDRALRLDSRVMLVDDPVKRVDNMTMAWGLEA
                     RVPFLDHDLFELAAACPPELKLAQDGKGVLKEASRRVIPNEVIDREKGYFPVPALKHL
                     EGRYLEMVREALHAPEARERGIFRKDYVQRLLDAPNDEKTRLGSNKLWQLGLLELWLQ
                     TRGV"
BASE COUNT         1205 a         2198 c         2374 g         1244 t
ORIGIN      
        1 tcacccgcgt gccggtgggc gcgatgccca tgagcacacc ttcttgcgtg ccctgccccg
       61 tccagcgggt caccacctcg tcgtcttccg cgagctggtc ctctatggtg aagtgggtgt
      121 ccgggtaggc gctgcggtac atgtcgacgt actgcctgat gccatcgggg ccgcgcacct
      181 ctccgggtac ggccggatcg tgcaggacat agtcgggggc gaacagttcg tccacaagct
      241 ccaggtctcc tcctccaaag acctcttcga gaaaccggcg gatgacggcc ttgttctctt
      301 cttgagacac cggatgttct ccttccaaat cgcggttact ttccctacca ccgcaatttg
      361 accccggacc gatgctcacc tgacagagca aagaatgctc actttatgct caaattatgg
      421 gacttttgtc ctattggccg ccgatctctt tgtgccacag gatctcgccc acccgcgtga
      481 tcccgccctt gagatggcgc cggtaggtgc tgaaggggag gtccagaact tcggcggcga
      541 gttgctgggt gggagccgga ttgaggtagg tatgatacag ggcgcggtaa cacttcgcct
      601 cccgcggcga cgctcgtagc gactccgcgg cttctttgag gaggtcccgc agagcggaga
      661 tactcacgct gtcgtccgcg ccggccccgg cgcgctcggc gactgtccgt gagtgcagga
      721 gcgggttggc gtgcagcgcc tcagagctat ggaagtcgcg cagtgcatcc cggatggccg
      781 tcttgaactc gggttcgccg aggactataa gcggctcggt ctctgtggag tgggacgcat
      841 cgagcggttc cgttgagatc tcccgttccg ccaggaggtc tagccaggcc atcggcggca
      901 tcaccctcca gtcgtgcgtg tacgtcccgt agtggtggcc gtccacctcg aaatcggctt
      961 caggagtccg cgccaggtcg aagtatgcga acgcgggcgc ccagaaatcg gggtcggcgg
     1021 tggggaagaa cgtgaaggcg aggtggggtg tagtcaggta gtgctgcgcg acattaccga
     1081 agatcaggct ctgcatcgcc gagacctcct ggtaggtgtc gcgcgccatc cagaaacgga
     1141 agagcgtgga cctctcgccg ggacgcagcg gcgcgtttcg ctccaggtac cgccacgcca
     1201 cctctaccgc ggggtccgcc tccctgtctt ctgcgctggt ctcttgcagt gccaccttca
     1261 taacgaaccc ggccgggcgc tgttcgatgt cgcggaagat cacgactccc cccggctgcc
     1321 ggtcgaacca gcgggatgcc aggcgcgccg attcagggcc ttcgtgctcc tcgatcatct
     1381 cccgcagggc aggtctgtcg ttctcgcgca tggtatcggg tagcgtggtt ccggtctcct
     1441 gccattcgag gaagggtttg atcatcgcgt tgtcccggtg caggtacatg tagtcgaaga
     1501 gcacgcgctg ctgttcctgg gcgtgcgtct gcttcaggcg cgccgcgtag tagttgcggg
     1561 cccgatggtg aagctcagcg taccagtcgg ggttgcgcca gcgcaggtcc gcgacgagcg
     1621 cctcgcgcgc cacgtcgtgc gggaagagcc cgagcggccc cgactcgatg aacgtaaggc
     1681 cgcgcagcca ctcgaacagc tcgtggacct cgggcatcgt caccatatga cccagcaggc
     1741 tctcggtcgt gtagcgtacc agcgcgcagg actccagcgc cgtgcggtga gccgggcccg
     1801 gcacctgctg cacgaactgc tccaggagcg tcttgatcac gtccggcgtc tccgagggtc
     1861 tgaagcgtac gccggaacgc tggacgaaca catccgcgac cagggagagt gcgagcggat
     1921 gaccgtgagt aaactccagg atgtcctggt gctgttcgga agggacctcg cgccggttta
     1981 ggtacgcctg gctctcttcg aggctcaggt ttcgcagttg caccgggtgg ataagccgct
     2041 gccagccggg atccgttcgc cagttcgtcc cgagcgggtc gcggctcgcc agtacgagga
     2101 gcaggttctc cggaagctga ggcagaaaga cttcgctcat ccaggagtcc agcggagcga
     2161 gggtctcgta agtgtcgatc aggacgaccc ggcgctcggt tgtcgtctct attgagccgg
     2221 gggatccatc gcgcatggcg ggacccagcg ctgaagatat ggcccccagg aaagcgtcgg
     2281 acgagggctc gacgttgcgc gcgtccacgt agctcgccgg gacgccgaat tcttcgcaga
     2341 tgttggcgaa ctcgcggagc aaggtggtct tgcccacgcc gccgggtccg gacacgtaga
     2401 gcacgttgaa aggagatcca tccgccgaga gcgcggaccg gaagagatcc cgctcagcaa
     2461 accgcccgac gaatctcctg tggcgctccg cgttgagacg gcttgccagg cccggtgaca
     2521 tagactacat ccttcgtgga cttaggcccc atcgttgcat tggaattatg ctgcgaaccg
     2581 tgggttgaat caacagcggt acgcgcgttc gaaagcggtt atgagggtta taggccgatt
     2641 gtcctagacg gaccggaacc gaacagacga tcttgccgcc gaaatatagc ctgtcacaaa
     2701 gcgaagcgag gaccgctctg gcatcttgct ctaggataaa atgtagatag aagagccaga
     2761 gatgaaaggt ttgagttggc tgatacgcag cagatcacct gggacccgga ggcggaccgc
     2821 aggatcaagc gcagcccgtt cctgatgcgg cgctttatcc gcaagcgcat cgaggaccgc
     2881 gtcgcttccc gtggacggaa ccacgtcacg gaggccgacg tggacgagag cgcgggcatg
     2941 taccggcagt accgcttcaa gtaaccggcg gagcgcgcta gccgtggggg ttgcgggagg
     3001 agggtttcgg ggaaacatag agccatccac gaccgagccg gagatcagag gggagacacg
     3061 gagatgagcg agcagagcca acaggaggcc cgtagcccgc tggtgagcga ccgcggcgtt
     3121 accaccataa gcgataaggt cgtctcgcag atagcgggca tggccgcgag agaggtgcgg
     3181 ggtctgcatc tcggcggcag cgcgtcgcgg acaaccgggg gactactgga gaacgtgacg
     3241 gggtcttcca gcagcacgcg cggcatctcc gtcgaggtcg ggcgagtgga ggccgccctg
     3301 gaccttaccc ttagcgtaga ttacgggacg gatattcttc cggctctcga agaggcgcgg
     3361 cgtaagatct ccgaccgcgt gagctccatg acggggctcc gagtaacaga gtgcaacgtg
     3421 accatcagcg acatcgttct cccgacgagc gacggggacg gcgagtctca gggcaaacgc
     3481 cgggatgaac tggagggtgg agcccgaacc ggggcccgtc cgaacgcgcg cacggtcgag
     3541 ctggaatctg aggtcgggga ccgcgaggtg ggagaggtcg agccgcgctc ccggacgcgc
     3601 acggaccttg ccggcggacc ggcccccgag gaggtccgcg tggaaggcga gccggtggac
     3661 gaggacgaga ccgtccggat ggacgccaga gatctccggg acgaagagcg gggccgtggg
     3721 aggcgccgcg accgccgccg cgataactaa cgcgcgcgct gtaagaacca gaccggctgg
     3781 atagactctc tgtatgcccc cggacctcct gcggcccgga ctcgcgctcg tgttctgcgg
     3841 gtacaaccca tcgctggcgt cgggccggac cggccgccac tacgcccatc ccggcaaccg
     3901 cttctggcgc gtactgcacg ccgccggtat cacggagcgt ctctattctc cggaggagga
     3961 cgaggaactg ctcgaacgcg gcatcggctt cacgaacctc tgcgcgcgac cgacgcgccg
     4021 ggctgacgag ttgagcaagg cggagatacg taagggctcc ggagagctgc tcgcccggct
     4081 cgaaagtctc cgccccgcag ccgtcgccta caccggcatc ggcgtctaca ggtggtttcg
     4141 ggcgacctcg aaagtcgagt ggggcgtgca gatggctccg gccgtcccgg aagttacaga
     4201 tgtggtggtt ccatctccct ccggcctcaa ccggatgcgc ttcgaggaac tggtcgttca
     4261 ttaccgtaag ctggcgccgt tcgtgtcctg atcgggccgt gcgtccggac aaaaaatatc
     4321 cgggtcggcg agccgcctca ctctacagcg gacgaccacc ggcccggctc acggacttac
     4381 gtccgcgaat ggttgtcgac ttagtagtcc atcggaccct ttcctcctag tcgaggtaca
     4441 acaaccaact aatatcgtat catctctggc gaacttcgcg ccagccttga acgtacatat
     4501 ttcggaccat gtcgtggaaa cggctagtct tcgggaactg aacccaggat ctcctctccg
     4561 agttctcctt cggggttggc gcgggtcggg agacgttcga cgaagggcag caggtcggtc
     4621 ctgacgacct cgaagaccct gcgaagttcg gcaaccggtt cggcgtgctc atccacgcgc
     4681 agatccaggt acgagtatgg ctcgtgatgg acgacgtaga tggctgcgct ctgccgtccg
     4741 cgccggtcgc cgcccgcctc ctgtccggct tcgagggcgc gcatcagacg ctccgggaac
     4801 cactcgccag ccgatgcctc gaacgcctcg gccatcgagg agatcgtcgc ctccccgacc
     4861 agcaggttcc ccgccaccga gtatccctct cccgtatagt gcccgcgcca ctcgttacag
     4921 tcctctcccg tataggccgc cgcgcgccca cgggagtcga tgacgctcaa ctggcggcgc
     4981 tccggttcgt ggtcggagtc gagtagagcg cggatggtct tctccgcgcc gtagccctgt
     5041 tgcagcatgt ttaggccgtc gaggccgaag agcgggttgg cgtgggcctg ggtcacgatc
     5101 gctccgacgc ccgctcgggc gtagttggtg atcgcgccga cagccgggac cttcgtactc
     5161 acggagatac cgtacatgcc ggtctccggg tcgtgcgcgg ctatggagaa cgtgttgtag
     5221 ttcagcagcg ggagcctcct tctcggccgg ttcagagccg catattagcg gaagaaccgc
     5281 cggtgcgaga ggctttgccg catgaggcta gacgccgcgc gtctggagcc agagttccag
     5341 gaggcccagt tgccagagtt tgttggagcc gagccgggtc ttctcgtcgt tcggcgcgtc
     5401 gagcaggcgc tgaacgtaat ccttgcggaa gataccgcgc tccctcgcct ccggggcgtg
     5461 cagcgcctcc cgcaccatct ccaggtagcg accttcgagg tgcttgaggg ccgggacggg
     5521 gaagtaaccc ttctcccggt ctatgacctc gtttgggatg acgcggcggg aggcttcttt
     5581 caagacgccc ttgccgtcct gggcgagctt gagctccggc gggcaggcgg cggccagctc
     5641 gaagaggtcg tggtccagga acgggacgcg ggcttcgagg ccccaggcca tcgtcatgtt
     5701 gtccacccgc tttacggggt cgtccacgag catcacccgg ctgtcgaggc gcagggcgcg
     5761 gtccagcggc gtgtcggccc ccggacggga gaagtgccgc ccggcgaact cccggctcac
     5821 gtcctcgccg gccaggtact ccgggttcag ggcctcctct atctcggagc ggggccggtc
     5881 gaagaactcg ccctcgtagg tgtcgaggcc gtggccctcg gcgtcctcca tcggcgggta
     5941 ccagtcgtag cccgcgaaga cctcgtcggc gccctggccg gattgcacga ccttcacgtg
     6001 ctgggcgacc tcgcgggaga gcaggtagaa gccgaccacg tcgtggctga ccatcggctc
     6061 gctcatcgcg gagatcgcgc cgtgcagcgc ggggagcatc cgctcgctgg agacgcggag
     6121 ttggtggtgg tcggtgccgt agtgcttcgc gacgaggtcc gagtacttga actcgtcgcc
     6181 ctcgatgccg ccgaccgact cgaacccgac gctgaaggtc tggatgtctt tctggcccag
     6241 ctccgcgagc agcccgacga ttatgcttga atccaggccg ccggagagca gcaccccgac
     6301 cggcacgtcg gcgaccatgc gccgctcgac ggcgacgcgc agcgcctcca ggacggcgtc
     6361 ctcccagtcc cgcgcgctca tgccctcgtg ggcggggtcg cgggtgaagc tcgggctcca
     6421 gtactcgcgg gtcttccggc tcccgtccgg ctcgatggtg agcagggtgg cgggagcgag
     6481 acggcggacg cccttcagga tcgtgttcgg cgccgggacg acggcgtgga acatcatgta
     6541 gtggtgcagg gccaccgggt ctatggaggt gtccacgccg ccaccggaca ggagggcggg
     6601 gagggtagag gcgaagcgca gggaatcgcc ggtatcggac agatagagcg gtttgatgcc
     6661 gaggcggtcg cggccgagga tggcgcgtcc cgagtcgcgc tcgacgatgc agaaggcgaa
     6721 catgcccttg agatgatcca cgaagttatc gccccagcgg tggtacgcct tcagcagcac
     6781 ctcggtgtcg ctggtcgaga agaagcggta gccgtgtttt attagctcct cgcgcagctc
     6841 cgggtagttg tagatgcagc cgttgaagac gatggtcaga ccgagctccg ggtcggtcat
     6901 cggctgggcg gcgcactcgg agaggtctat gatcttcaac cgccggtggc cgagtgccac
     6961 gccgccctgg ctccagaacc cgccgccgtc cgggccgcgg tcgttcatcg tctcgttcat
     7021 c
//
LOCUS       DASSMB010000168        15282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_546357, whole genome shotgun sequence.
ACCESSION   DASSMB010000168 DASSMB010000000
VERSION     DASSMB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15282
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546357"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VFJ72_09955"
     CDS             complement(<1..1470)
                     /locus_tag="VFJ72_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7479821.1"
                     /translation="MAFDKVRPQVNFPKLEEDVLDLWRREKTFEKSVQSRPEDQPFVF
                     YEGPPTANGRPGFHHALARAFKDLIPRYKTMQGYRVVRKGGWDTHGLPVELQVEKELG
                     LTGKDDIERYGVEEFNKLCRESVFRYVEDWQKMSERLGFWVDMDDPYKTLDNSYIQSV
                     WWALKTLHDRGLLYEGYKVSAHCPRDQTSLSSAEVALGYQQYPDPVVDPSVYVRLPLA
                     SPDEDNTSLLIWTTTPWTLISNAAVAVHPNVEYATVEVDGERLVIATELLDKVLGEGD
                     HHIVARTKGRDLEGKKYRRPFDYVPVQESENLWTVVLGEYVTTAEGTGLVHTAPAYGE
                     DDARVGRRYGLPTIHPVKPDGTFDERVGPFAGQFVKDANKGLVEELRERGLLFRAEDY
                     EHAYPHCWRCGTPLLYYAKKAWYARTTAVLDDLLSENEGINWVPDHIKWGRFGDWLKN
                     NIDWALSRERYWGTPLPIWRTESGATVVIGSAEELRELAI"
     gene            1973..3658
                     /locus_tag="VFJ72_09960"
     CDS             1973..3658
                     /locus_tag="VFJ72_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7479822.1"
                     /translation="MSSRLRAGIAGAAGAVVAFGLTELVHGLFERIPSIFVSLAQAVI
                     KLTPGEFVTRGIETLGTADIPVLVSSLVVGALIVAGLLGILSVRRPLVALLVVGVLAV
                     IAITAAFAQPFIAPVPTILTVIVSLWAGCTVTSALLSAAGLRAGKSKSETAPAGPADD
                     PRSPVMRSREAHSEGGIAVGRGGFLLLSGGAAAAGLAAVGAGRLLAGQNAPEAAAPRK
                     LKLPEAPKPASNKSSAKSKEDGAQKVAVKHQTLPPPDPKASLDVPGMPPLITPAKDFY
                     LIDTVLQSPRIDVNTWKLSVKGAVDNPVEFSYKDLLGMSTREVDVTLSCVSNEVGGGL
                     ISNGRWTGVLLSDVLKEAGVSRDKISRASRQLVGRSVDGWTSGFKTEIALDGREALVA
                     FGLNGSELPIKHGYPVRLVVPGLYGYVSATKWITEIELTNWNFDAYWIQRTWSKEGPI
                     KTQSRFDTVKDGDNLSAGKVQVGGVAWAPHRGISKVEVSTDGGSSWNEARLAKQLDID
                     TWRQYVYDWDARPGDYTLQVRATDGDGQTQTSQTAPPHPSGATGYDTIQVSVA"
     gene            3954..4649
                     /gene="nfi"
                     /locus_tag="VFJ72_09965"
     CDS             3954..4649
                     /gene="nfi"
                     /locus_tag="VFJ72_09965"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565365.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HET7479823.1"
                     /translation="MKVNKLHSFEIPPSEARQLQVDLAPKVARGPTLDLGSVRYVAGT
                     DVSTQGEKGYAAVVVLEFPGLSVVEARGFEAPLEFPYVPGLLSFREMPSLIGALELVE
                     TPVDAIIVDGQGYAHPRRMGIASHLGLFVDVPTVGCAKSVLVGKYEEPGSEKGSVTDL
                     VHRGEVVGRVVRTRDRVSPVYVSAGNRIDLESAVDLVLSCCPKYRLPETTRQAHNAAN
                     RLRRGEAVGDLLA"
     gene            4779..6485
                     /locus_tag="VFJ72_09970"
     CDS             4779..6485
                     /locus_tag="VFJ72_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7479824.1"
                     /translation="MAPDKSIGAISRRDFLRLSGVGLAGAALIGVAGCGGGGTISGGQ
                     GGSGGGGGNTFIFGRGGDSVTLDPPHTTDTESSKVTRQIFDGLLDFAPESTDVAPALA
                     TEVPKPENGGKEYTFKLRKGVKFHDGAPFNADAVVTNFERWRFTKNPLHKGGGSQGGD
                     FSYYQAQFNGFDDDSIIKKVEAVDDYTVKITLGEPQGPFLRNIAMSNFTMASPKALKK
                     DVENFWQNPVGTGPFKFVKWDKGSEVQLEKNPDWWGTDLPASEGGGGPNVDKVVFKSI
                     PDNTSRVAALNGGDLSAADGLTPDDIPTVEDGQGLKIDYRPPLTIGYLAMNTQKKPFG
                     DPKVRQAINYAIDMEKITKAFFGDTGQVASTYMPPTVPFFDKSIKPYPYDPDKAKQLL
                     KEAGQENLETELWYMPIPRPYMPDGKGIAQVMQNDLEKIGMKIKLQTFEWGTYLDKTG
                     RGEHPMALLGWTGDNGDPDNFLNVLLSSTTATKTDALNIAYYKNPEVDKILKDAQGSI
                     DQSERQKLYYQAQEMLVKDAPWAPIAYAKVPVGLKKDVSGYQPSPTGGESFNNISLGG
                     GK"
     gene            6561..7550
                     /locus_tag="VFJ72_09975"
     CDS             6561..7550
                     /locus_tag="VFJ72_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479825.1"
                     /translation="MGRRLIQIIPVLLGVSILVFFMVRAIPGDPAQLLLGQNATEQQV
                     QQLRAKLGLDKPQVVQYAVFLKDAATGDLGDSIVTGRSVTAELAARLPATVELTAFAM
                     LIAVIVGVPVGVISAVKQYSLLDKIASVFALTGISMPIFWLAMILIVIFAVNLQIFPF
                     PGRLGSDTSISAITGLVLVDSLLTLNFSGFWDGLMHLILPALALGTIPMAVVTRMTRS
                     SMLEVMNEDYVRTARAKGVVPWRVIFKHALRNAMLPTVTVIGLQVGLLMGGAVITETI
                     FAWPGVGQIAYQSINRRDYAMIQGVVLYGALIFILINLLVDVLYAVLDPRVRY"
     gene            7552..8454
                     /locus_tag="VFJ72_09980"
     CDS             7552..8454
                     /locus_tag="VFJ72_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479826.1"
                     /translation="MAEASAGVTVTTGEVRARSRREDFRRAFLGNRLAVFGAVVLALF
                     ILMAVFAPFIAPYDPLEQEITSKFAPPSGKHLLGQDEIGRDVLSRIIYGARISLTAGL
                     AAVAISTVMGTVVGVIAGFFGGWLDSVLMRIMDVILAFPSILLAIVIVTILGPSLPNA
                     MLAVGIVFIPQMARVVRSAVITIRERDYIEAERAIGAGNAQIVFSGVLPNSLAPLIVQ
                     STLTLATAILDVAALSFLGLGAQPPSPEWGAMLTDAFKSGYSVFINGQHAILFPGIAI
                     ALSVLAVNFIGDGLRDALDPRRRR"
     gene            8539..9174
                     /locus_tag="VFJ72_09985"
     CDS             8539..9174
                     /locus_tag="VFJ72_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HET7479827.1"
                     /translation="MRSRRFGSWTRTKGKTVYYRRRRWSGRSVLVLFGTALILLSVGM
                     LLSGFHILGGSLPAPKDESLRLTVPKMQRVKNVPVYTAASSDTAKLDAGAIHLKDTGY
                     PWQAGSNVYIAGHRLGYARTGSFLLFYDLNRLQDGDTVVLKDTEGRKYIYRVFNEVVV
                     APDDLSVTKPIAGKSVVSLQACTLPDYSHRLIVQAELIKTVEQPAPQASSG"
     gene            complement(9178..10116)
                     /locus_tag="VFJ72_09990"
     CDS             complement(9178..10116)
                     /locus_tag="VFJ72_09990"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479828.1"
                     /translation="MNDAVWVLTLAAAVSAGTPLVFAAVGEILAERSGVLNLGVEGMM
                     LLGAVTAFLAADASGSPLVGLLAGTLAGGGLALVHAFLSVTMRANQIVSGLALVIFGT
                     GLATFLGNPIEGDPLKPGVTNFRIPGLAEIPVLGKILFDQDPLVYVSWVVVALAAFYL
                     TRTRTGLALRACGESPETADAMGLSVARLRYAHVIIGGLLAGAGGAYQILARVPSWSG
                     AGTTAGIGWIALALVVFAAWRPWRALLGAYLFGAALAANFALQAAGVNIIPAEFLSML
                     PYLLTILVLVALSASSARKRLGAPAALGVPYAREER"
     gene            complement(10113..11228)
                     /locus_tag="VFJ72_09995"
     CDS             complement(10113..11228)
                     /locus_tag="VFJ72_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7479829.1"
                     /translation="MQVRLERRLETRRGHRALVTVLSVVAALLLGAVFLSLTGKSPLA
                     VYTEMFRAAFLSFYGITDSLAVAIPLILTGLAAAVAFRMQLYNIGAEGQLYVGAILGS
                     WAALALTPGLPNPLAIIIVFIFGAVGGALWILPPALARAWLGTSEIITTLLLNFVALY
                     LMRYLIYGSASYWRDPTTTNFPQGKPIPEEAFLPVVTLTRVHLGLAVALLAAVVLWVL
                     LSRTRFGYDVRVVGDSPGAARYAGISVRRTIIIVMLTSGALAGLAGAGEVAGIAHTLD
                     PNGLAVNFGYTGIIVAALARSNPFGVVAVAILLGGLQNAGTALQSIPGGAGVPVEVSY
                     MLQGAILLCALAGELFIRYRLRVRRGLPDELIEGTKP"
     gene            complement(11230..12735)
                     /locus_tag="VFJ72_10000"
     CDS             complement(11230..12735)
                     /locus_tag="VFJ72_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7479830.1"
                     /translation="MPDAPPAVEARAVSKAFPGVIANARVDLSVERGEVHAILGENGA
                     GKSTLASILAGLYRPDEGEVRIDGEPVHFGSPRDALARGVGMVYQHFRLVERFTVAEN
                     VVLGDPRQPMLLSTRRAEKTVAELGEKYGLPVHPRARVGNLSVGEQQRVEIVKMLYRG
                     ADVLILDEPTAVLTPQESENLFETVNAMAAEGKSVIFISHKLSEVLAVSHHVTVMRDG
                     HVVARAETALSDRRSLARAMVGRDVDLSVQRAEAAPGEPLLELENLNAEGEGDTVPLR
                     DVSLKVRAGEVVGVAGVAGNGQRSLAEVAAGMRTPSSGRVVVRGVDTTGKGPKAARRA
                     GLAYVPEDRLGTGLAPSLSISENLLLTHPRPFFLKRKEMEGTAREAIEQFGIRAPGPD
                     AATRGLSGGNAQKALLARELSDGPEVIIVASPTRGLDVGATQAVREILDKRRRAGCAV
                     LLISEDLDEVRSLSDRILVLYEGRIVYETIGEDADVEDLGLAMAGSDTGGH"
     gene            complement(12742..13896)
                     /locus_tag="VFJ72_10005"
     CDS             complement(12742..13896)
                     /locus_tag="VFJ72_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7479831.1"
                     /translation="MMTNNPNLKTRRQFLAGAGLTLGAIGASGLLSACSSGGGSGSSG
                     GGSGDKLKVAFVYIGPTGDAGWTTRHDDGRKMLQKELGDKVETSFVENVPEGATAQRT
                     FEDLARKGNKLIFGTSFGYMDSMLAAAKKYPDTVFMHATGFKTAKNLGTYAGGAEQAS
                     YLAGIAAGKAAKDGKMGYLAPFPIPEVIRGLNALMLGARSVNPKATMQVVWTSTWFDP
                     DKEKQAAQGLTNSGVDVIGMQQDSPAAGKVAQNANDKWVGFNDDMKRFAPEAWLTGPM
                     WNWGPFYIKTAKQVLNDTWKTEQYYGGMDDGLVQLAPFGKSVSEDTRKMIEEKKQATI
                     DGKFHAFQGPVKDQDGKVRIPKGEKASLDDLLSMDYLVEGVIGKIPKNAE"
     gene            complement(13893..14921)
                     /locus_tag="VFJ72_10010"
     CDS             complement(13893..14921)
                     /locus_tag="VFJ72_10010"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET7479832.1"
                     /translation="MQQLRGANEAIEAYHASLEPFWHPVVPSAELGDGPVAIELLGRG
                     LVLARLEGEAAAFDDVCRHLGAALSGGQVVEGGSHLRCPYHGWSYDRSGRCVDIPARR
                     GAAIPREARVRSYATREQYGLIWVCLDEEPEHEIPALPEFDDPEVHKGPLRTYGPWRA
                     SAPRIVMAALDDTHFPWVHPGILGDPAHPEPPDHRVYREGEYLVSTYDMMQPANDTIS
                     SNGGAGALEKVTYTNYVTATSIRLVKESAAGVYVIWQVASPIAHDRSLIFSRQARSFD
                     RDPARDPEYESLQDTILEQDRPIVESQRPWLLPPLSSRMMLYVRPADLPIVAFQKWLE
                     ELDVPQEV"
BASE COUNT         2613 a         4917 c         5096 g         2656 t
ORIGIN      
        1 tatcgccagt tcccggagtt cctccgcgct cccgatcacg accgtcgcgc cggactctgt
       61 ccgccagatg gggagcggcg tgccccagta gcgctcgcgg cttagggccc agtctatgtt
      121 gttcttcagc cagtctccga atcggcccca ctttatgtgg tcggggaccc agttgatccc
      181 ctcgttctcc gagagcaggt catccagcac ggccgtagtc cgggcgtacc aggccttctt
      241 ggcgtagtag aggagcggcg tcccgcatct ccagcagtgc gggtaggcgt gctcgtagtc
      301 ctccgcccgg aacagcaacc cacgctcacg aagctcctcg accagtccct tgttggcgtc
      361 cttcacgaac tgcccggcga acggcccgac ccgctcgtcg aacgtgccgt cgggctttac
      421 cgggtggatg gtcggcagcc cgtagcgccg tccgacacgc gcgtcgtcct cgccgtaggc
      481 gggggccgta tggacgaggc cggtcccctc ggccgtagtt acgtactcgc cgagcacgac
      541 ggtccacagg ttctccgact cctggacggg tacgtagtcg aacggacgcc ggtacttctt
      601 gccctcaagg tcgcggccct tcgtcctggc gactatgtga tggtctccct cgcctagcac
      661 cttatccaga agctcggttg cgatcacgag tctctccccg tccacctcga cggtcgcgta
      721 ctcgacgttt gggtggacgg cgacggcggc gtttgagatc agggtccagg gcgtggtggt
      781 ccagatgagg aggctggtat tgtcttcgtc cggcgaagcc agcgggagcc tgacgtagac
      841 gctcgggtcc acgacggggt ccgggtactg ctggtagccg agggccactt ctgctgagga
      901 gagtgaggtt tgatctctcg ggcagtgcgc gctgaccttg tagccttcgt agagcaggcc
      961 cctgtcgtgg agtgtcttga gtgcccacca gacgctctgg atgtaggagt tgtcaagggt
     1021 cttgtatggg tcgtccatgt ccacccagaa accgagccgc tcgctcatct tctgccagtc
     1081 ctcgacgtag cggaagacgg actcgcggca cagcttgttg aactcctcga cgccgtagcg
     1141 ctcgatgtcg tccttgccgg tgaggccgag ttccttctcc acctggagtt ccacgggcag
     1201 gccgtgcgtg tcccagccgc ccttacgcac gacccggtat ccctgcatgg tcttgtagcg
     1261 ggggatcagg tccttgaaag ccctcgccag cgcgtggtgg aaccccggcc ggccgttggc
     1321 cgtcggcggt ccctcgtaga acacgaaggg ctggtcctcg ggacggctct gtacggactt
     1381 ctcgaacgtc ttctcccgcc gccagaggtc cagcacgtcc tcctccagct tcgggaagtt
     1441 gacctgcgga cgaaccttgt cgaatgccat gcacgctcct caatatgaaa gctcccgtcc
     1501 ccggctaacg ccagacggag cgacgcttca ctccaccata gggacgggag cccgaagagc
     1561 tctcgcggta ccaccctaat tgcccggcta ctccgggcct ctcgcgcgcc gcggcataag
     1621 ccccggcacc cacgctgtaa cgggcgtacc cggccggtcc tactaaacgt ctaggctttg
     1681 ccttccgctt tcggccggcg gctctgggag gatcttctgg ccgtctctcg cgccgggctc
     1741 cacaccgtcc ccggctcgct ctggcgaggt cccacgaccg tactcgttcc cgtcgctgcc
     1801 tgtaaagagc tttgtagcct ggaaaaggag gcgtgtcaag ggtgggctat tgaggatgat
     1861 ccagaccttc cctcggattc gtaactattg tgtaagaact ctgtaagaac cgggtgtaat
     1921 actgagtatc tgaaggtgct cagagctagt cgagagtatt ggagtgacag tcgtgagcag
     1981 taggttgcgg gcgggaatag cgggagcggc gggggcggtg gtggcgtttg gcctcacgga
     2041 gctggtacac gggctcttcg agcgcatacc gtcgatattc gtctctctcg cccaggcggt
     2101 tataaagctg acgccgggcg agttcgtgac tcggggcatt gagaccctgg gcacggcgga
     2161 catcccggtg ctggtgagct cgctggtggt cggagctttg atcgtcgccg gacttctcgg
     2221 cattttgagc gtccggcgtc cactcgtcgc gctgctggta gtcggcgtcc tggcggtcat
     2281 cgccataacc gccgccttcg cgcaaccttt tatcgcgccc gtcccaacta tcctgaccgt
     2341 catcgtctcc ctctgggccg gctgcaccgt cacctcggcc ctgctctcgg ctgccggtct
     2401 ccgtgccggg aagtccaaat ccgagaccgc gccggcggga ccggccgatg atccgcgctc
     2461 cccggtcatg cgttcgagag aggcgcactc ggagggcggg atagcggtcg ggcggggagg
     2521 gtttctgctc ttgagtggag gagccgccgc cgcgggactc gcggccgtgg gagcgggccg
     2581 gttgctcgcc ggacagaacg cgccggaggc agccgccccc aggaagctga agctcccgga
     2641 agctccgaag cccgcgtcga acaagtcttc cgcgaaatcc aaagaagacg gcgcgcagaa
     2701 ggtcgccgta aagcaccaga cgcttccgcc accggacccc aaagcctcgc tcgacgtgcc
     2761 ggggatgccg cccctgatca cgccggcaaa ggacttctat ctcatagaca cggtccttca
     2821 gtcgccgcgc atagacgtca atacctggaa gctctccgtg aagggcgccg tggataaccc
     2881 cgtcgagttc tcctataagg acctgctcgg catgtccacc cgcgaggtgg atgtaacgct
     2941 ctcgtgcgtc tccaacgagg tggggggagg tttgatctcg aacggccgct ggaccggagt
     3001 cctgctctcg gacgtcctca aagaggccgg agtgagccgg gataagatct cccgcgcctc
     3061 ccgccagctc gtgggccgca gcgtggacgg ctggacctcg ggcttcaaga cggagatcgc
     3121 gctggacggc cgcgaggccc tcgtcgcctt cggcctgaat ggctcggagc ttcccatcaa
     3181 gcacggctac ccggtgcggc tggtggtgcc tgggctgtac ggttacgttt cggcgacgaa
     3241 gtggatcacc gagatagagc tcacgaactg gaacttcgac gcttactgga tccaacgcac
     3301 ctggtccaag gaaggtccca tcaagaccca gtctcgcttc gacaccgtga aggacggcga
     3361 taacctctcc gccggcaagg tccaggtcgg cggcgtcgcc tgggcccctc accgcggcat
     3421 ctcgaaggtc gaggtctcca ccgatggcgg ctcctcctgg aacgaggccc gcctcgccaa
     3481 acaactcgac atagacacct ggcgccagta cgtctacgac tgggacgcca gacccggcga
     3541 ctacacactc caggtccgcg ccaccgacgg cgacggccag acccagacct cgcagacagc
     3601 ccctccacac ccatccggag ccaccggata cgacacgatc caggtctccg tcgcctgaga
     3661 aagctctacc gtgctaacct tctaccgaaa cttgagcttt aggacgaaaa tgcgccgtaa
     3721 cgtatcggca agcaccaaaa tggggttctg taatcaaagt gtttcctcta cgtaacttaa
     3781 ccgtaacatc ctggcaatgg aaacgtaaca gcacccaggt atattgcgtt taaccgagcc
     3841 ccggccggtg ggacgttccc ccctccagat cccatcggtc gggacggtct ttttgacagt
     3901 tcctttcaga cctgttccag tgtgggctac atccgtatta ctctggtgcg ttaatgaagg
     3961 ttaacaaact acactccttc gaaatccctc cgtccgaggc ccgccaactc caggtagacc
     4021 ttgctccgaa agtggctcgg gggccgacgc tggacctcgg gagcgtgagg tatgtcgcgg
     4081 gcacggacgt ttcgacacag ggagaaaagg gctatgccgc cgtcgtcgtt ctggaatttc
     4141 cggggctctc ggtcgtcgag gctcgggggt tcgaggcgcc tctggagttc ccgtatgttc
     4201 cggggctgct gtccttccgg gagatgccgt ccctgatcgg agcgctggag cttgtagaga
     4261 ctccggtgga cgccattatc gtggacgggc aggggtacgc ccatccgcgg cggatgggga
     4321 tcgcgtcgca cctggggctg ttcgtggacg tgccgacggt ggggtgcgcc aagagcgtcc
     4381 tcgttgggaa gtacgaggaa ccgggaagcg agaagggaag cgttacggac ctcgtgcatc
     4441 gtggcgaggt ggtcgggagg gtggtcagga cgcgggaccg ggtctcgccg gtctacgtgt
     4501 ccgcggggaa caggatagac ctggagagcg cggtagacct ggtgctttcc tgctgcccga
     4561 agtaccggct gccggagacc acccggcagg cgcacaacgc cgccaaccgg ctgcggcgtg
     4621 gggaggctgt aggggaccta ctggcgtagc gtctacgggc gtagcgccgg tgctaaatca
     4681 gtgttaactt actcgcattg catccaggtg gacaaatact tcgtaaaaag taagtgaggc
     4741 agtaagaaca atcgcgggtt tcgtgaaagg ggcttgattt ggcaccggat aagagcatag
     4801 gggcgatcag ccgcagggac tttctgaggt tgagtggtgt ggggctcgcc ggggccgccc
     4861 tgataggcgt ggccggctgc ggcggtggcg ggaccatctc cggcggtcag ggcggttccg
     4921 gtggcggcgg tggcaacacc ttcatcttcg gacgcggggg cgactcggtt acgctggacc
     4981 cgccgcacac cacggacacc gagtcctcca aggttacgcg ccagatcttc gacgggctcc
     5041 tggacttcgc gccggagagc acggacgtcg cgccagcgct ggcgaccgag gtcccgaagc
     5101 ccgagaacgg cggcaaggag tacaccttca agctgcgcaa gggcgtcaag ttccacgatg
     5161 gcgcgccgtt caacgcggac gccgtcgtca ccaacttcga gcggtggcgg tttacgaaga
     5221 acccgctgca caaaggtggc ggcagccagg gcggagactt ctcctactat caggcgcagt
     5281 tcaacggctt cgacgatgac tcgatcatca agaaggtcga ggctgtggac gactacacgg
     5341 tgaagatcac gctcggcgag ccccagggac cgttcctgcg gaacatcgcc atgagcaact
     5401 tcacgatggc ctccccgaag gcgctcaaga aggacgtcga gaacttctgg cagaacccgg
     5461 ttgggacggg accgttcaag ttcgtgaagt gggacaaggg ctccgaggtc cagttggaga
     5521 agaaccccga ctggtggggg acggacctgc ccgcctccga gggtggcggc ggaccgaacg
     5581 tggacaaggt ggttttcaag tcaatcccgg acaatacctc gcgggtggcg gcgctaaacg
     5641 gcggagacct ctccgctgcc gacggcctga ccccggacga catcccgacg gtagaggacg
     5701 gccaggggct gaagatcgac taccggccgc cgctgaccat cgggtatctg gcgatgaaca
     5761 cccagaagaa gccgttcggc gatcccaagg tgcgccaggc cataaactac gccatcgaca
     5821 tggagaagat cacgaaggcc ttcttcgggg ataccgggca ggtcgcctcg acctacatgc
     5881 cgccgaccgt cccgttcttc gacaagagca tcaagcccta tccgtatgac ccggacaagg
     5941 ccaagcagct tctgaaagag gccggccagg agaacctgga gaccgagctg tggtatatgc
     6001 cgataccgcg tccgtacatg ccggacggca agggcatcgc gcaggtcatg cagaacgacc
     6061 tggagaagat cggcatgaag atcaagctcc agaccttcga gtgggggacc tacctggaca
     6121 agacgggccg gggcgagcac ccgatggccc tgctgggctg gacgggcgac aacggcgacc
     6181 cggacaattt cctgaacgtg ctcctctcca gcacgaccgc caccaagacg gacgcgctca
     6241 acatcgccta ctacaagaac ccggaagtag acaagatcct caaggacgcc caggggagca
     6301 tagaccagag cgagcgccag aagctctact accaggctca ggagatgctc gtgaaggacg
     6361 ctccgtgggc gccgatagcc tacgcgaagg tccccgtcgg cctgaagaaa gacgtttcgg
     6421 gctaccagcc gagcccgacc ggcggcgagt cgttcaacaa catctccctc ggcggcggca
     6481 agtaagggcg ggatcaggga gcgggccgtt cttcggcccg ctccgagttc ttataggagg
     6541 ttggggctct gttagagttt gtgggacggc ggctcatcca gatcatcccc gttctgctgg
     6601 gggtatccat cctcgtcttc ttcatggtgc gagctatccc cggagacccc gcgcagctac
     6661 tcctgggtca gaacgcgacc gaacagcagg tgcagcagct ccgggccaag ctcggcctgg
     6721 acaagcccca ggtagtgcag tacgcggtct tcctgaaaga cgccgccacc ggagacctgg
     6781 gcgactccat cgtgacgggc cgctccgtga cggcggagct ggcggcgagg cttccggcga
     6841 cggtagagct cacggcgttc gcgatgctca tagcggtcat cgtcggggtg ccggtggggg
     6901 tgatcagcgc ggtcaagcag tactcgttgc tggacaagat cgcctcggtg ttcgcgctca
     6961 ccgggatcag catgcccatc ttctggctgg cgatgatcct gatcgtcatc ttcgccgtga
     7021 acctgcagat cttcccgttc cccggacgat taggtagcga cacttccatc tccgcgatca
     7081 ccggcctggt gctcgtggac tcgctgctca cgctcaactt ctcgggtttc tgggacgggc
     7141 tgatgcacct gatccttccg gcgctcgcgc tcggcacaat cccgatggcg gtcgtcaccc
     7201 gcatgacgcg gagcagcatg ctggaggtca tgaacgagga ttacgtgcgg acggcgcggg
     7261 cgaagggcgt cgtgccgtgg cgcgtaatct tcaagcacgc tctcaggaac gccatgctcc
     7321 cgacggtcac ggtcatcggg cttcaggtgg ggctcctgat gggcggcgcg gtcataacgg
     7381 agaccatctt cgcctggccg ggggtcggcc agatcgccta ccagtccatc aaccgccgcg
     7441 actacgccat gatccagggc gtggtcctct acggagccct gatattcata ctgatcaacc
     7501 tgctggtgga cgtgctctac gccgtcctcg acccgagggt gaggtactag agtggccgag
     7561 gcgagcgctg gcgtaaccgt caccaccgga gaggtccggg cgcggagccg ccgcgaggac
     7621 ttccggcgag cgttcttggg caaccgtctc gcggtcttcg gggccgtggt gctggcgttg
     7681 ttcatcctga tggccgtgtt cgcgccgttt atagcgccct acgacccgct ggagcaggag
     7741 ataacctcca agttcgcgcc gccgagcggg aagcacctgc tggggcagga tgagatcggc
     7801 cgggacgtac tctcgcgcat catttatggc gccaggatct cgctgactgc cggtctcgcg
     7861 gcggtcgcca tctcgaccgt catggggacc gtggtcgggg tgatcgccgg gttcttcggc
     7921 ggctggctgg actctgttct gatgcggatc atggacgtga tcctggcgtt cccgagcatc
     7981 ttgctagcca tcgtgatcgt cacgatcctc gggccgagcc tgccaaacgc gatgctggcc
     8041 gtcggtatcg tctttatccc gcagatggct cgtgtggtgc gctcggcggt cataaccatc
     8101 cgggagcgcg actacataga ggccgagcgg gcgatagggg cggggaacgc ccagatcgtg
     8161 ttctccggcg tcttgccgaa cagcttggcg cccctgatcg tgcagtccac cctcacgctg
     8221 gcgaccgcga tcctggacgt ggcggcgctc tctttcctgg gactcggcgc gcagccgccc
     8281 tcgccggagt ggggcgctat gctgaccgac gccttcaaga gcggctacag cgtctttatc
     8341 aacggccagc acgccatcct cttccccggc atcgccatcg ctctctccgt gctggcggtg
     8401 aacttcatcg gggacgggct gcgcgacgcg ctggacccgc gccgccggcg ctagagcctt
     8461 cgccgctccc cgccggaggg tgaacaaagc cgctatttga taccgtcttg taatcctttt
     8521 gcggtatcat aggtcgccgt gagatccagg cgtttcgggt cgtggacccg caccaaaggg
     8581 aagaccgttt attatcggcg gcggcgctgg tccggccgga gcgtcctggt gctcttcggg
     8641 acggccctga tcctgctctc cgtcggcatg ctgctctccg gcttccacat cctcggcggc
     8701 tcgcttccgg ctccgaaaga cgagagcctg cgcctcacgg tcccgaagat gcagcgcgtc
     8761 aagaacgtgc ccgtctacac cgcggcctcc agcgacaccg cgaagctaga cgccggggcc
     8821 atccatctca aggataccgg ctacccgtgg caggcgggct cgaacgtcta catagccgga
     8881 catcgcctcg gctacgcgcg gaccggcagc ttcctgctct tctacgacct gaaccggctg
     8941 caagacggtg atacggtagt gctcaaggat accgaggggc gcaaatacat ctaccgggtc
     9001 ttcaacgagg tcgtggtcgc accggatgac ctctccgtca cgaagcccat cgccggcaag
     9061 agcgtcgtca gcctgcaggc ctgcacgctc ccggactact cgcaccgcct gatcgtgcag
     9121 gccgagctta taaagaccgt cgagcagccg gcgccgcagg ccagctccgg ctaacgctta
     9181 tcgctcctca cgggcgtagg ggacgccgag ggcggcgggc gcgccgaggc gcttgcgggc
     9241 gctggatgcg gatagggcca cgagcaccag gatcgtcagc aggtagggaa gcatcgagag
     9301 gaactcggcg ggtatgatgt tcaccccggc ggcctgcagc gcgaagttgg cggcgagagc
     9361 ggcgccgaag aggtaggcgc cgaggagcgc ccgccaggga cgccacgcgg cgaagacgac
     9421 gagggccagc gcgatccagc cgatcccggc cgtcgtgccc gcgccgctcc acgacgggac
     9481 ccgcgccagg atctggtacg cgcccccggc cccggcgagc agccccccaa taatgacgtg
     9541 cgcgtagcgc aggcgggcca cgctcaggcc catcgcgtcg gccgtctccg gcgactctcc
     9601 gcacgctcgc agcgcgagcc ctgtccgcgt gcgcgtcagg tagaaggccg cgagcgcgac
     9661 gacgacccag ctaacgtaga ctagcgggtc ctggtcgaag aggatcttcc cgagaaccgg
     9721 tatctccgcg aggccgggta tgcggaagtt agtgacgccc ggtttcaagg ggtctccctc
     9781 gatgggattg ccgaggaagg tggcgagccc ggtcccgaag atgacgaggg cgaggccgct
     9841 gacgatctgg ttggcccgca tcgtgacgct gaggaaggcg tgcaccagcg ccagtccgcc
     9901 gcccgcgagt gtcccggcga ggagcccgac cagagggctg ccggaggcgt cggccgccag
     9961 gaaggcggtt acggccccga ggagcatcat cccctccacg cccaggttga ggacgccgga
    10021 ccgctcggcc agtatctcgc cgacggcggc gaagacgagc ggcgtcccgg cagagacggc
    10081 ggccgccagc gtaagcaccc agacggcgtc gttcacggtt tcgttccctc gatcaactcg
    10141 tccggcaggc cgcggcggac gcgcagacgg tagcggataa acagttcacc ggcaagggcg
    10201 cagagcagga tcgcgccctg caacatgtac gagacctcga ccggaacccc ggcgcccccc
    10261 ggaatgctct gcaaagctgt ccccgcgttc tgcaaaccgc ccagcaggat cgccaccgcg
    10321 accacgccga acgggttgga ccgggccagc gccgcgacga tgatgcccgt gtagccgaag
    10381 ttcaccgcga ggccgttcgg gtctagagta tgcgcgatcc ctgcgacctc gcccgctccg
    10441 gcgagtccgg ccagagcgcc ggaggtgagc attactatga tgatggtgcg tcgcacggag
    10501 atgcccgcgt agcgcgccgc gccgggcgag tcgccgacga cccgcacgtc gtagccgaag
    10561 cgggtgcggg agagcaggac ccagagcacc accgccgcga gcagggccac ggcgaggccg
    10621 agatgcaccc gcgtgagcgt gacaacggga aggaacgcct cctccgggat cggcttcccc
    10681 tgcgggaagt tggtcgtcgt cgggtcgcgc cagtaggagg cggagccgta gatcaggtag
    10741 cgcatcaggt acagggcgac gaagttcaga aggagcgtcg tgatgatctc cgaggtcccg
    10801 agccaagctc gggcgagggc tggaggtagt atccacagcg cgccgcccac cgctccgaag
    10861 atgaacacga tgattatcgc gagtgggttc ggcagtcccg gcgtcagggc gagcgcggcc
    10921 caggaaccga ggatcgcgcc gacgtagagc tgtccctccg ccccgatgtt gtagagctgc
    10981 atcctgaacg ccaccgccgc cgcgagcccg gtcaggatga gcggtatcgc caccgcgagc
    11041 gagtcggtga tgccgtagaa agagaggaac gccgcccgga acatctcggt gtacacggcg
    11101 agcggggact tgccggtgag ggagaggaag accgcgccca gcaggagcgc cgccacgacc
    11161 gagaggacgg tgacgagcgc ccggtgcccg cggcgggttt cgaggcggcg ttcgagtcga
    11221 acctgcatat cagtgtccgc cggtgtcgga gccggccata gccaggccca ggtcttccac
    11281 gtccgcgtcc tcgccgatcg tctcgtagac gatgcggccc tcgtagagga ccaggatcct
    11341 atcggagagg gaacgcacct cgtccaggtc ctcggagatg aggagcaccg cgcacccggc
    11401 ccgccgcctt ttgtccagga tctcccgcac cgcctgcgtc gccccaacgt cgaggccgcg
    11461 ggtgggggag gccacgatga tgacctccgg accgtcggag agctcgcggg ccaggagcgc
    11521 tttctgggcg ttgccgccgg agaggccgcg cgttgcggcg tcggggccgg gagcccggat
    11581 cccgaactgc tctatagcct cgcgggccgt gccttccatc tccttgcgct ttaggaagaa
    11641 cggcctcgga tgcgtgagca acaggttctc ggagatggag agcgacggcg cgagccccgt
    11701 acccagccgg tcctccggga cgtaggcgag ccccgcccgc cgcgccgcct tcggtccctt
    11761 gccggtcgtg tccacgccgc gcaccacgac ccgtcccgac gacggcgtcc gcatcccggc
    11821 agctacctcg gcgagcgacc gctgtccgtt acccgcgacc cccgccacgc cgaccacctc
    11881 cccggcccgc accttcaggc tcacgtcccg gagcggcacg gtgtcgccct cgccctcggc
    11941 gttcaggttc tcgagttcga ggagcggttc gcccggtgct gcctcggcgc gttgtacgga
    12001 gaggtccacg tcgcggccga ccatcgcgcg agcgagggag cggcggtcgg agagggcggt
    12061 ctcggcgcgg gcgacgacgt gtccgtcgcg catgacggtt acgtggtggg agacggcgag
    12121 gacctcggag agcttgtggc tgatgaagat gacggacttg ccctcggcgg ccatcgcgtt
    12181 cacggtctcg aagagattct cggactcctg gggggtgagg acggcggtcg gttcgtccag
    12241 gatcaggaca tccgcgcccc ggtagagcat cttgacgatc tccacgcgct gctgctcccc
    12301 gaccgacagg ttcccgaccc gcgcccgggg gtgaacgggc agaccatatt tctcccccag
    12361 ctccgcgacg gtcttctcgg cgcggcgggt ggagagaagc atcggctggc gcggatctcc
    12421 gaggacgacg ttctctgcca cggtgaagcg ctcgacgagg cggaagtgct ggtagaccat
    12481 gccgacgccg cgcgccaggg cgtcgcgcgg gctgccgaag tggacgggct cgccgtctat
    12541 gcgcacctcg ccctcgtcgg ggcggtagag gccggcgagg atggaggcca gggtgctctt
    12601 gccggctccg ttctcgccga ggatggcgtg cacctcgccg cgctccaccg agaggtccac
    12661 cctggcgttg gcgatcacgc cggggaaagc cttggagacg gccctcgcct cgaccgccgg
    12721 cggcgcgtcc ggcacggggt actactccgc gttcttcggg atcttaccta tgacgccctc
    12781 gaccagatag tccatcgaga ggaggtcgtc gagcgaagcc ttctcgccct tcgggatgcg
    12841 gaccttgccg tcctggtcct ttacgggacc ctggaaggcg tggaacttgc cgtctatggt
    12901 ggcctgcttc ttctcctcga tcatcttccg ggtgtcctcg ctgacggact tgccgaacgg
    12961 cgcgagctgc acgaggccgt catccatgcc cccgtagtac tgctcggtct tccaggtgtc
    13021 gttgaggacc tgcttggcgg tctttatgta gaacggtccc cagttccaca tcggtccggt
    13081 gagccaggct tcgggggcga accttttcat gtcgtcgttg aagccgaccc acttgtcgtt
    13141 ggcattctgg gcgaccttgc cggcggcggg gctgtcctgc tgcatcccga tcacgtccac
    13201 accggagttc gtgagcccct gggcggcctg cttctccttg tccgggtcga accacgtcga
    13261 ggtccacacg acctgcatcg tcgccttcgg gttgacggag cgggcgccga gcatgagggc
    13321 gttgaggccc cggatgacct ccgggatggg gaagggggcg aggtaaccca tcttgccgtc
    13381 cttcgccgcc ttgcccgccg cgatgccggc caggtaggag gcctgctccg cgcccccggc
    13441 gtaggtgccg aggttcttgg cggtcttgaa gccggtggcg tgcatgaaga cggtgtccgg
    13501 gtacttcttg gcggcggcga gcatcgagtc catgtagccg aagctggtcc cgaagatgag
    13561 cttgttaccc ttgcgggcca ggtcttcgaa ggtccgctgc gccgtcgcgc cctctggcac
    13621 gttctcgacg aagctggtct cgaccttgtc tccgagctct ttctggagca tcttgcggcc
    13681 gtcgtcgtgg cgggtagtcc agccggcgtc gccggtcggg ccgatgtaca cgaaggcgac
    13741 cttcagcttg tctcccgagc cgccgccgga ggagccgctc cctccaccgc tgctgcacgc
    13801 ggagaggagt ccgctcgcgc cgatggcgcc tagggtcagg ccggcgccgg cgaggaactg
    13861 gcggcgggtc ttgaggttgg ggttgttcgt catcatacct cctgtgggac gtctagctct
    13921 tcgagccatt tttgaaaggc aactatagga aggtccgcgg gcctcacgta gagcatcata
    13981 cgcgaggaga gaggggggag gagccaggga cgctggctct cgactatggg gcggtcctgc
    14041 tccagtatgg tgtcctgaag actctcatac tcagggtcgc gggctgggtc ccggtcaaag
    14101 gaacgggcct ggcgcgagaa gataagggag cggtcgtgcg cgatggggga ggcgacctgc
    14161 cagatcacat acacaccagc cgcgctctcc ttgacgagcc ggatgctcgt agccgtcacg
    14221 tagttcgtat acgtgacctt ctctaaagcg cccgcgccgc cgttcgaact gatggtgtcg
    14281 ttggccggct gcatcatgtc ataggtggag acgaggtact cgccctcccg gtagacccgg
    14341 tggtccggcg gctcgggatg cgcggggtct ccgagtatcc ccggatgcac ccacgggaaa
    14401 tgcgtgtcgt ccagcgcggc catcacgatg cgcggcgcgc tcgcccgcca cgggccgtag
    14461 gtgcgcagcg gtcccttgtg gacctccgga tcgtcgaact cggggagcgc cggtatctca
    14521 tgctccggct cctcgtcgag acagacccag atgaggccat actgctcgcg agtcgcataa
    14581 gaacgcaccc gcgcctcgcg gggtatggcc gcgccgcgcc gagcggggat gtccacgcag
    14641 cggcccgacc tgtcgtagct ccagccgtgg tacgggcagc gcaggtggga gccaccttcc
    14701 acgacctgcc caccggagag cgccgcgccc aggtgccggc agacgtcgtc gaaggcggcg
    14761 gcttctccct ccagccgcgc caggaccagt ccgcgtccca gcagttcgat agcgaccggg
    14821 ccgtccccta gctcggcgga gggaacgacg gggtgccaga acggttcgag gctggcatga
    14881 tatgcctcta tcgcctcgtt cgctccccgt aactgctgca agaaacctcc ctgacggctg
    14941 taacgactgg acgtatcttt tgcgaaagat accaggaaaa gataccgtct gtcacgcgcc
    15001 aactctttgt gcaaaaggcc aggattcatc gcggaagaac tgtcccgtgg tatagagcaa
    15061 aagggcgagt tgcgtgatcg aggaaccgga cctccgaagg gcgtatcctt ctggctgtgg
    15121 gcttctacag aggaggtttc ggagatggcg gagcgcagac ctttatacga gatgacccgg
    15181 acgctcgcgg caacggcgcg gggggaggag ccggcggatc tggtcgtgcg gaacggcacg
    15241 ctggtgagcg tcacctccgg cgagatagtg ccggggatga gc
//
LOCUS       DASSMB010000169        13406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_420934, whole genome shotgun sequence.
ACCESSION   DASSMB010000169 DASSMB010000000
VERSION     DASSMB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13406
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420934"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFJ72_10015"
     CDS             <1..528
                     /locus_tag="VFJ72_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479833.1"
                     /translation="KFSGRLFAGLEYAAVLGRQAGFEVETAHTGDLFMLRVAAGPGSE
                     AGAKFGLLPGAAAETDEDLMHEELSRYSLDPSGYSGRLLGWSEAAGEDACQTFAVYRD
                     GVWKTKGLFVTKARGKVDDPDEVLNGFSLRIFGRLVDLASLIGGAGKSWADEPYAFRD
                     LAGGRPFPTQVRFPR"
     gene            complement(533..865)
                     /locus_tag="VFJ72_10020"
     CDS             complement(533..865)
                     /locus_tag="VFJ72_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479834.1"
                     /translation="MPRDLPEVTLLSDARGERPENAAGIGAFWYEPEVWTLPLSPAAR
                     VLYAGLCSYLGHGQINYRDLRSAVKGRKDEEIAEAFEELIHHNLLLPDGAPSAAAYAV
                     RSVREFEG"
     gene            complement(865..3390)
                     /locus_tag="VFJ72_10025"
     CDS             complement(865..3390)
                     /locus_tag="VFJ72_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7479835.1"
                     /translation="MSDPIPRGEETDAGASDKEPDKLLAKRLPPEGLPEELLQRRLGR
                     LDTRRALAELCKSIPGLDEVLLPRGIQADALTRKTGGSAQMVRTISRKILQDAAAWEA
                     FRTAIHEQIPPETFDAVENFSKERIEELTGAHTTEGLLLAALSAEHEPEDGTIAALIE
                     AWKDEHQRAEKKASEDALVQELKADIERLKKENEKLSFTSRTANERAAALAEEVAVLT
                     GEREGASTLVRQAEERAAAALDVRSQLNERVAELERRGANLERALESERRAYSTAAVR
                     VEEMHEELGRVAAERDRIQNALENARFTDEGFGELLVRAVKNEVVALPNDLESANHTA
                     RLLEFMGKVLHAHNDLRSPNAPDREPRKPGSRSRAAKSRQGEDSDESESSPADPTELR
                     RVPASTLSNGLAGGRVVETARARTRPTLSFRALGGAGEVGGSSHLLDFGRTRVLVDAG
                     IKPDGRGPATPDFGSLDGLDAAVITHAHLDHCGALPVLVRDRPDLPIYCTPPSARLIV
                     AALNDHAAMGGGLPGGAPIHEVGKRLVPVPFGRPISVGDTKITLTESGHILGAASVLL
                     RSGSATVFHTGDICMEDHFSIPSAKLPEVRDIDLLIMEATLADQRPQPFSESIHTMIE
                     VINDTILERDGTVLIPTYALGQAQEIILGLKHYGREYGLDRDVFIYVDGSVVTTSERL
                     YAEQLGYMKPYLQHSDPRELFFSENIRAVANDDRARERILTNPCAIIASPVTMQGGAS
                     AFYRRRLEKDATNAVILPSNAAASYGAHKAVGEEEQWRVERVSFAAHCTQEDLLGITE
                     KLSPKQIILIHGSRRRISDLAFRLAPSHKIHTPTVGETVRTIL"
     gene            3512..3778
                     /locus_tag="VFJ72_10030"
     CDS             3512..3778
                     /locus_tag="VFJ72_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479836.1"
                     /translation="MGEASERGSVKVRAHSPGRYPILILELENGELVAAYHETGYDLS
                     RTKSVENDWVRENAIGRHSFVEVEPPVEVSPSALRDYARGLRAR"
     gene            complement(3794..5788)
                     /gene="pknB"
                     /locus_tag="VFJ72_10035"
     CDS             complement(3794..5788)
                     /gene="pknB"
                     /locus_tag="VFJ72_10035"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET7479837.1"
                     /translation="MQQLVDNRYRIVRELGSGGMAEVYLAHDDVLDRDVALKVMSGRY
                     ADDDEFVERFRREAQSAAALSHPNIVSIYDRGASEDGTYYIAMEYLPGGTLKDRILKR
                     GALQPRTAAAVTMQIAEALQVAHKHGVIHRDIKPHNILITESGDLKVTDFGIARAASS
                     STMTKTGSILGTAHYISPEQAMGEHVGPASDLYSLGVVLYEMLTGELPYDADTPIGIA
                     MKHVNGHLRPPKAMDPSIPDGINAITVRLLAKDPMDRYGSAAELIEDLERFIAGRQPA
                     SATTQMMAAQTTAQTLYAPTRVGPPPSRIPERRKERRSIAPLLLILLALALLAPVAYA
                     TGLLGGSQDTPTRQPKGTKAPAKVKVPELTGLKLDEARRQYGDLKIVEDDRRESKEAV
                     GTILEQDPASGEKVEKGSEVSVVVSGTQVADVPDVKGDQQDDARSALEDAGFKVKTEE
                     KESSDADKGLIIEQDPEGGSTEKVGSEVTITVGTGPEKIKVPDIPYGATASEAQSLLE
                     SNGLVLGGQSSRHNGQIAKGGVIDQSPRPGAEVEPGSAVSITLSSGPKQIAVPDVVGQ
                     NVDVAKQAMVDAGLGYKSKEVQSNKPAGTVISTDPAAGTMLDPLTYVLITYSIGPPEQ
                     TTKPSPKPAPKPKPKQKDSGSVQDRIDQIKKDAKKSVKGG"
     gene            complement(5807..7240)
                     /locus_tag="VFJ72_10040"
     CDS             complement(5807..7240)
                     /locus_tag="VFJ72_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7479838.1"
                     /translation="MNAQLRRSFYVFVAGFVALVAMMAYWQVYARESLANNPNNSLQT
                     RRSLEAPRGLILAGDGETVLARSDKRETSTGTVYDRVYPQGDLYSNIVGYWSVKYGAT
                     GIEIGENSTLSGNSDPATLDELINQISGGPQAGNNVELTLDPNLQRVAYDGLAASNTG
                     QGSAVAIDPKTGDILAMVSYPSFDANNIDERFQELQQDPNKPLLNRATQGLYTPGSVM
                     KVITAAAALKAGVKPTDIFFDSGRYETPGYTVVNYGDASYGRVTFAQALALSINVVFA
                     KVAVEDIGAQLLYDTAKNFGFGDTYENFPLEINPSTLGVPVSDMVGGTLAQTSFGQDR
                     VQSNVFEFANVAAAIANDGRMMEPRIVKEIRSPEGIILDKPAPSTRADVIDRETALTL
                     QDMMVGVVDEGSATGAQIPGVKVAGKTGTAEVPPRDPNSWFIAFAPADDPKIAVAVLV
                     ENGGDGEKQALPIARQIIEASLKTGGG"
     gene            complement(7237..8538)
                     /locus_tag="VFJ72_10045"
     CDS             complement(7237..8538)
                     /locus_tag="VFJ72_10045"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET7479839.1"
                     /translation="MTQRATMPMILALAITTLGFATLIFVQEATSPPLIYGAYYAGTL
                     FALYLAVRLFLPHSDVLLLPIVTLLTGIGLVMIFRLTYDVPDVENLATTQAVWILIGS
                     GTLLFIVLFFRNYGRLFDYKYLFALAAVVLIASTIFFGYEVNGAQLWLRVGPISFQPS
                     EFARLALIIFFAGYLAEKRDLLAATSRSFLGVQIPTLKYFGPVAFVWAASLGLLVFEK
                     DLGSSLLFFAVPLLMLYVATGRIAYVILGGLLFCVGSYASYLLFGHVQVRVETWLDPW
                     KDPNGGGFQIVQSLFNIAEGGLTGTGLGAGFAQTIPEVQTDFIFSAIASELGLLGATA
                     VLLAFLVFVYRGTKISLLATDDASKLLAFGLTAMFALQTLIIVGGVTKAIPLTGITLP
                     FISYGGSSVVGNFILTGLLLVISEKAGKSADGDEHAYEDMP"
     gene            complement(8549..9739)
                     /locus_tag="VFJ72_10050"
     CDS             complement(8549..9739)
                     /locus_tag="VFJ72_10050"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET7479840.1"
                     /translation="MLFLEHFGVTDAGKVRKNNEDSLLVGDGKDETLFAVADGIGGFE
                     AGEVASSITVDVLREMEPGDSFEEAVREANRRILEAAHNDERLSGMGTTVVAARFSGT
                     QRDPVAEIAHVGDSRAYLLRDGDLKPVTEDHSLVAELVRSGDLTRAQASNHPQRNLIT
                     RAIGADEDVEVDTSTLSVRANDRIILCSDGLSDMVPEDRIREILIAAPDEPETPARRL
                     LTAALEAGGTDNITVIVVDVRKQSPQEERSGTQEMQAVSAPEDVTRSPEPPREKTPSR
                     RERRDAAKSAAKRRRKGPREGFTRTAGNLTRALAIVLVVLIALAPVYLWGSSRYFLDL
                     DGGEVVAYRGLPYAPLGIHLNEEWRRTNIKESEIKAPYRDNVTEHKLYTRDETEKILR
                     DLRS"
     gene            complement(9749..10246)
                     /locus_tag="VFJ72_10055"
     CDS             complement(9749..10246)
                     /locus_tag="VFJ72_10055"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7479841.1"
                     /translation="MLELQVPLLAAKALLLLLLFAFIYAVVRRGVGDLRDIPDDESYE
                     PGSSRGAGRNGRGAYVPPPGKDPVAELIVEDSAIIAPRTRFPIDSGTTSLGRSSASDI
                     VLKSDDFASGKHAMLTRHGGLLYVEDVGSTNGTFVNGRKTVGATPLRSGDKVKVGSTT
                     FRYEE"
     gene            complement(10247..11134)
                     /locus_tag="VFJ72_10060"
     CDS             complement(10247..11134)
                     /locus_tag="VFJ72_10060"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HET7479842.1"
                     /translation="MGFLKQIEKRMESLVEGVFGRAFRRQIHPVEIAKGLTKQMDENR
                     MVSISRTYAPNDFTIHLSQADTESIQAYKDSLRDELIQYASTHAQNKSYHLMSPPRIR
                     FETEESLRFGEFGVTAKLTGGSGPREKGAPQDTSGQTRIFRTDTGEEEQNIEGTAAIS
                     AEDARKQGLAREVVELVLDGKPHPLDGPGPWSIGRSEENDITINDPNVSRKHARLLRA
                     HNGFIIEDLGSTNGTLLDGAPIDRERIDNGDELTFGQSTARFVRRDSDSPGGGRPGGE
                     QPGEPGGVTSRQNRTSGSR"
     gene            complement(11142..12749)
                     /locus_tag="VFJ72_10065"
     CDS             complement(11142..12749)
                     /locus_tag="VFJ72_10065"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7479843.1"
                     /translation="MAETRYIEGQGRYALAEMVGRGGFATVWRARSFGGSFTFGSRGN
                     DVAIKIIPIYSSQERSRAMREGQIAEGLRHKNIVETIEVIPGDTEIYLVTEYVRGLPL
                     DEAARDYDVNQVINALAQVLEALVYAHAQGIIHRDIKPQNALVDGRGRVKLTDFGVAY
                     RAGDTRLTRIGYAVGTPGYIAPEIMDGADPSVLSDIYAVGATARTLLAYQPQEPPRRL
                     REFIDRATSPNPAHRPQSAWAALKLLTGRRAPAPYPSKASAPKPNKKPTREERRVAGS
                     LPEQALRLANGLAASWLGYQLGTDLLLQNGAQAVGIAAGLGVAGFLLPRLAALGLVVA
                     LAVLLFKNGVGLGFSLLVPMLGGLWIAGTGSSRSASRCPLGPVLAIPLALVGLGAGLP
                     LLFGYLMRPVGACVSAAMGAATLICYDLTIGDGVLPYFGVPFQKVPGALAPMELIQRG
                     QDILQAYPPLPALAMLWAAMAGVVSVAEWTGRWATGLVLAAGGGALAYALVISTSRNS
                     LMEAMTSLGLASIIYGVVRYLGSRVRG"
     gene            12899..12982
                     /locus_tag="VFJ72_10070"
     tRNA            12899..12982
                     /locus_tag="VFJ72_10070"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12933..12935,aa:Leu,seq:cag)
     gene            13210..>13406
                     /locus_tag="VFJ72_10075"
     CDS             13210..>13406
                     /locus_tag="VFJ72_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479844.1"
                     /translation="MRKYVTSSASLLSDSSNAGKQDLQNTLANAGGDASKLDGEALRN
                     ASDNAQLLYQRALDNDEVPPE"
BASE COUNT         2084 a         4342 c         4414 g         2566 t
ORIGIN      
        1 aagttctccg ggcgcctctt cgccgggctc gagtacgcgg cggtgctggg acggcaggcc
       61 gggttcgagg tagagaccgc tcataccgga gacctcttca tgctccgggt ggccgccggg
      121 ccgggctcgg aggcgggcgc caagttcggg ctactgccgg gcgccgccgc cgagactgac
      181 gaggacttga tgcacgagga gttgagccgc tacagcctgg acccttccgg ctactcggga
      241 cgtcttctcg gatggtccga ggccgccgga gaagacgcct gtcagacctt cgccgtctac
      301 cgcgacggcg tctggaagac aaagggcctc ttcgtgacga aagctcgcgg taaggtggac
      361 gacccggatg aagtgctcaa cggcttctcc ctgcggatat tcgggcgtct cgtggatcta
      421 gcctccctca tcggcggcgc gggaaagagt tgggcggacg agccatacgc tttccgggat
      481 ctcgccggcg gcaggccctt cccgacacag gtacgctttc ctcgctaact cttcagccct
      541 cgaactcgcg cacggagcgc acggcgtacg cggcggcgct tggagcccca tcaggtagaa
      601 gcaggttgtg atggatcagc tcctcgaacg cttcggctat ctcttcgtct ttgcggccct
      661 tcacagcgga gcggaggtcg cgatagttga tctggccgtg tcccaggtac gagcagagtc
      721 cggcatagag gacgcgggcg gcgggggaga ggggtagcgt ccagacctcc ggctcgtacc
      781 agaacgcccc gatcccggcg gcgttctccg ggcgctcgcc gcgagcgtcc gagagcagcg
      841 tgacctccgg cagatcccgc ggcactacag gatcgtgcgg accgtctcgc cgacggttgg
      901 ggtgtggatc ttatggctcg gcgccaggcg gaaggcgagg tcgctgatgc gcctgcggga
      961 gccgtggatc aggatgatct gcttcggcga gagcttctct gttatgccga gcaggtcttc
     1021 ctgcgtgcag tgcgccgcga agctgacccg ctcgactcgc cactgctcct cttcgccgac
     1081 ggccttgtgg gcgccgtagg atgcggcggc gttggacggc aggatcacgg cgttcgtggc
     1141 gtctttctcc aggcggcgcc ggtagaacgc gctcgctcca ccctgcatcg tgaccggcga
     1201 ggcgatgatg gcgcacgggt tcgtcaggat ccgctcccgc gcccggtcat cgttggcgac
     1261 ggcccgaatg ttctcggaga agaataactc tctggggtcc gagtgctgca ggtagggctt
     1321 catgtagccg agttgctcgg cgtagagcct ctctgacgtg gtcaccaccg agccgtccac
     1381 gtagatgaac acgtcacggt cgagcccgta ctccctgccg tagtgcttga ggccgaggat
     1441 gatctcctgc gcctgtccga gggcgtaggt cggtatgagg accgtcccgt cccgctccag
     1501 gatcgtgtcg ttgatgacct cgatcatcgt gtggatagac tcggagaagg gctgaggtct
     1561 ctggtccgcg agcgtggcct ccatgatcag gaggtctatg tccctgacct ctggcagctt
     1621 ggccgacggt atggagaagt ggtcttccat gcagatgtcg ccggtgtgga agactgtcgc
     1681 tgaaccagat cgcagcagca cgctggccgc ccccagtatg tgtccgctct ccgtgagcgt
     1741 tatcttcgtg tccccgacgc tgatgggcct gccgaacggc acgggcacga gccgtttgcc
     1801 gacctcatgg atcggggctc ctcccggcag accgccgccc attgcggcgt gatcgtttag
     1861 cgcggcgacg atcagccgcg ccgacggcgg cgtgcagtag atgggaaggt ccggccggtc
     1921 ccggaccagc accggcaggg ccccgcagtg atccaggtgc gcgtgcgtta taaccgccgc
     1981 gtcgagaccg tcgagcgatc caaagtccgg cgtggcggga ccccggccgt ccggctttat
     2041 accggcgtcc accagcaccc gcgtccgccc gaagtccagc aggtggctgc tgccaccgac
     2101 ctctcccgcc ccgcccagag cccggaacga gagtgtcggc cgcgtccttg cccgggccgt
     2161 ttctacgacg cgaccgccgg caagcccgtt cgacagggtg ctagccggga ctctacgtag
     2221 ctccgtcggg tccgctggag aagactccga ttcgtcagag tcttctccct ggcggctctt
     2281 ggccgcccgc gatctggatc cgggtttgcg cggctcccgg tctggagcgt tcggagagcg
     2341 gaggtcgtta tgagcgtgca gcaccttgcc catgaactcc agcaggcggg cggtgtggtt
     2401 cgccgactcc aggtcattcg gcagggcgac gacctcgttc tttaccgccc gcacgagcag
     2461 ctccccgaac ccttcatccg tgaagcgggc gttctccagg gcgttctgga tgcggtcccg
     2521 ctcggcggcg acgcggccca gttcctcgtg catctcctct acccgcacgg ccgccgtgga
     2581 gtacgcgcgg cgctcgctct ccagcgcccg ctccaggttg gcgccgcgcc gctctagctc
     2641 ggcgacccgc tcatttagct ggcttctcac atccagagcc gcggcggcgc gctcctcggc
     2701 ctgccgcact agcgtggagg ctccctctcg ctcgccggtc aagaccgcta cctcctcggc
     2761 gagggccgcg gcacgctcgt tggcggttcg agaagtaaag gagagcttct cattctcttt
     2821 cttcaggcgt tctatatcgg ccttgagttc ctgaacgaga gcgtcttcgg aagccttctt
     2881 ctcggcccgc tggtgctcgt ctttccacgc ctcgatcagg gccgctatcg tcccgtcttc
     2941 cggttcgtgt tcggcgctca gggccgcgag aagcagaccc tccgtagtgt gagccccggt
     3001 cagttcctcg atcctctcct tcgagaagtt ctcgacggcg tcgaaggtct cgggcggtat
     3061 ctgctcgtgg atggccgtgc gaaaggcctc ccaggcggcg gcgtcctgca ggatcttgcg
     3121 cgagatggta cgcaccatct gggcgctacc gccggtcttg cgcgtcaggg cgtcggcctg
     3181 gatgcctcgt ggcagcagca cctcatctag ccccggtatg ctcttacaca gctccgccag
     3241 ggcgcggcgg gtgtccagac ggcctagacg ccgctgtaga agctcctcgg gcaacccctc
     3301 cggcggcagc cgttttgcca ggagcttgtc cggttccttg tcggaggcgc cggcatcagt
     3361 ttcttcgcct ctgggtatgg gatctgacat gggaactccc tccggggata tgaatgtctc
     3421 gcagcgtctt catgagtaag gattttatca tggcggcttc ccggtcccgc tccggcttct
     3481 ttttggggta cgcttcgtat agacttcact tatgggcgaa gcatcggaga gaggttcagt
     3541 gaaagtccgg gcgcactcac ccggcagata tcctattctg atcctggagt tggagaacgg
     3601 cgagctggta gccgcttacc acgagaccgg ctacgacctg agccggacca agtccgtaga
     3661 gaacgattgg gtgcgcgaga atgccatcgg acgccacagc ttcgtcgagg tggagccgcc
     3721 ggtcgaggtg tctccctccg cgctacgaga ttacgcgcgc ggattacgcg cgcgatgaga
     3781 taaccagaga ggtttaacct ccctttacgg acttcttcgc gtccttcttg atctggtcta
     3841 tcctgtcctg gacgctaccg gagtctttct gcttcggttt aggtttcggg gcgggcttcg
     3901 gagacggttt cgtcgtctgc tccggcggac cgatgctgta ggtgatgagc acgtaggtga
     3961 gggggtccag catcgtgccc gccgccgggt ccgtggagat gaccgtcccc gccggcttgt
     4021 tgctctgaac ctccttgctc ttgtagccga gacccgcgtc taccatcgcc tgcttcgcca
     4081 cgtccacatt ctgtccgacc acgtccggca cggcgatctg cttgggcccg gagctcaatg
     4141 tgatgctcac ggcgctgccc ggctcgacct cggctccggg cctggggctc tgatctatga
     4201 cgccgccttt ggcgatctga ccgttgtgcc tgctgctctg gccgccgagg accaggccat
     4261 tgctctccag aaggctctga gcctccgagg ccgtggctcc gtatgggatg tccggtacct
     4321 tgatcttctc cgggccggtg ccgaccgtta tcgtgacctc cgagcctacc ttctctgtcg
     4381 aaccgccctc cgggtcctgc tctatgatca gacccttatc cgcgtcggag ctctccttct
     4441 cctccgtctt gaccttgaaa ccggcgtcct cgagcgcgct ccgggcgtca tcctgctgat
     4501 cacccttgac gtccggcacg tcggctacct gggtgccgct gacgactacc gagacctcgg
     4561 agcccttctc gactttctcg ccgctcgccg ggtcctgctc cagtatggtc ccaaccgcct
     4621 ccttgctctc cctcctgtcg tcctcgacga tcttcaggtc cccgtactgt cggcgcgcct
     4681 cgtcgagctt caggccggtc agttccggca cctttacctt cgccggcgcc ttggtccctt
     4741 tcggttgacg ggtgggcgta tcctgggagc ctcccagcag gccggttgcg taggccaccg
     4801 gggccagaag cgccagcgcg aggaggatca gcaagagcgg cgctatcgaa cggcgttcct
     4861 tgcgcctctc ggggatacga gagggcggcg ggcctaccct ggttggcgcg tagagcgtct
     4921 gggccgtcgt ttgagccgcc atcatctgtg tagtggcgct cgctggctgg cggcccgcga
     4981 taaagcgctc caggtcctca atgagttccg cggcgctacc gtagcggtcc atcgggtcct
     5041 tcgccagcag gcgcaccgtg atggcgttta tgccgtccgg tatggaaggg tccatcgcct
     5101 tcggggggcg caggtgtccg ttgacgtgct tcatcgctat gccgatgggc gtatccgcgt
     5161 cgtagggcag ctcgccggtc agcatctcgt acaggacgac gcccagagag tacaaatcgc
     5221 tcgccgggcc cacatgttcg cccatcgcct gctcgggcga gatgtagtga gccgtgccga
     5281 ggatggagcc cgtcttcgtc atcgtggacg atgaggcggc gcgggctatc ccgaagtccg
     5341 tgaccttgag atcgccggac tcggtgatga ggatgttgtg cggttttatg tcgcggtgga
     5401 taactccgtg tttgtgggct acctggagag cctccgctat ctgcatcgtc acggccgccg
     5461 ccgtgcgggg ctggagggcg ccgcgcttga gtatcctgtc ctttagcgtg ccgccgggca
     5521 ggtactccat cgcaatgtag taggtgccgt cctcggacgc tccgcggtcg tagatggaga
     5581 cgatgttcgg gtgcgagaga gcggcggcgc tctgcgcctc gcggcggaag cgttcgacga
     5641 actcgtcgtc gtcggcgtag cgcccgctca tcaccttgag ggccacgtct cggtccagga
     5701 cgtcgtcgtg agcgagatag acctcggcca tgccgcccga tccgagctcc cttactatcc
     5761 ggtaacgatt gtctactagc tgctgcatga gcccggcatt ataacttcaa ccgccgccgg
     5821 tcttcagact ggcctcgatg atctggcgcg ctatgggcag cgcctgcttc tctccgtcgc
     5881 ccccgttctc gaccaggacg gcgaccgcta tcttcgggtc gtcggccggc gcgaaggcga
     5941 tgaaccagga gttcgggtcc ctcggaggga cctccgccgt gcccgtcttg cccgccacct
     6001 tcacgcccgg aatctgagcc ccggtcgccg atccctcgtc cacgacgccg accatcatgt
     6061 cctgaagcgt cagggcggtc tcgcggtcta tcacgtccgc gcgggtgctc ggagccggtt
     6121 tgtcgaggat tatgccctcg ggagagcgga tctccttgac tatcctgggc tccatcatcc
     6181 tcccgtcgtt ggctatggcg gcggcaacgt tggcgaactc gaagacgttg ctctgcaccc
     6241 ggtcctgccc gaaggaggtc tgggccaggg tcccgccgac catgtccgag accgggacgc
     6301 caagcgtgct ggggttgatc tccagcggga agttctcgta ggtgtcgccg aaaccgaagt
     6361 ttttcgcggt gtcgtagagc aactgcgccc cgatgtcctc gacggcgacc ttggcgaaga
     6421 cgacgtttat ggagagggcg agcgcctgcg cgaaggtcac ccgtccgtag gaggcgtctc
     6481 cgtagttgac gacggtgtag cccggcgtct cgtacctgcc ggagtcgaag aagatgtccg
     6541 tcggtttcac gccggccttg agcgccgccg cggcggttat gaccttcatc accgagcccg
     6601 gcgtgtagag accctgggtc gcccggttga ggagtggttt gttcgggtcc tgctgaagct
     6661 cctgaaagcg ctcgtctatg ttgttcgcgt cgaaggaggg gtaggagacc atcgccagga
     6721 tgtctcccgt tttcgggtct atggcgaccg ccgagccctg cccggtgttg ctcgcggcga
     6781 gcccgtcgta ggcgacgcgc tggaggttcg ggtccagggt aagctcgacg ttgttgcccg
     6841 cctggggacc gccggagatc tggttgataa gctcatccag ggtcgcgggg tccgagttcc
     6901 cggagagggt cgagttctcc ccgatctcga tgcccgtcgc cccgtacttg acgctccagt
     6961 aaccgacgat gttggagtag aggtctccct gaggatagac ccggtcgtag acggtgccgg
     7021 tggacgtctc gcgcttgtcg cttcgggcga ggaccgtctc cccgtcaccg gcgagtatga
     7081 ggccgcgcgg cgcctccagg ctccggcgcg tctggagcga gttgttcggg ttgttggcga
     7141 gcgactcgcg cgcgtagacc tgccagtacg ccatcatcgc gaccagcgcc acgaaccccg
     7201 cgacgaacac gtagaatgag cgcctcagtt gcgcgttcaa ggcatgtcct cgtaggcgtg
     7261 ctcgtctccg tcggcgctct ttccggcctt ctccgagatg accagcagaa gccccgtcag
     7321 gatgaagttg ccgacgaccg aggagccccc gtaggagata aagggcagcg ttatgccggt
     7381 gagcggtatc gccttcgtca ccccgccgac gatgatgagc gtctgaaggg cgaacatggc
     7441 cgtcagcccg aaggccagga gcttcgaggc gtcgtcggtg gctagaagag atattttggt
     7501 cccccggtac acgaacacga ggaaggccag cagtaccgct gtcgctccca gcagccccag
     7561 ctcgctggct atcgcggaga agataaagtc cgtctgcacc tccgggatgg tctgggcgaa
     7621 tcccgcgccg agccccgtgc cggtcaggcc gccctcggcg atgttgaaga ggctctggac
     7681 gatctggaac ccgccgccgt tcgggtcctt ccacgggtcc agccatgtct cgacccgaac
     7741 ctggacgtga ccgaacagca ggtacgaggc gtaggagccg acgcagaaga gcagcccgcc
     7801 gaggatgacg taggcgatcc tgccggtcgc cacatagagc atcaggagcg gcacggcgaa
     7861 gaacaggaga ctgctgccga ggtctttttc gaagaccagc aggcccaaag aggcagccca
     7921 cacgaacgcg accggtccga agtacttgag cgtcgggatc tggactccga ggaagctgcg
     7981 gctggtggcc gcgagcaggt cacgcttctc cgccagatag ccggcgaaga aaattatgag
     8041 ggcgaggcgg gcgaactccg agggctggaa gctgatcggc ccgaccctca gccagagctg
     8101 cgcgccgttg acctcgtagc cgaagaagat cgtcgaggct attaggacga cggcggcgag
     8161 cgcgaagagg tacttgtagt cgaagaggcg cccgtagttg cggaagaaaa gcacgataaa
     8221 caacagggtg ccgctgccga taaggatcca gacggcctgc gtggtagcca ggttctccac
     8281 atccggcacg tcgtaggtga gccggaagat catgacgagc ccgatcccgg tgagtagcgt
     8341 cactatcggc aacaggagta cgtccgaatg cggcaggaat agcctgacgg ccaggtagag
     8401 cgcgaagagc gtccccgcgt agtaggcgcc gtagatgagc ggcgggctcg tggcttcctg
     8461 gacgaagata agggtcgcga accccagggt ggtgatagcg agggcgagga tcatcggcat
     8521 ggtggcgcgc tgggtcatat cttggcctct agctcctgag atcccgcagg atcttctcgg
     8581 tctcgtcgcg cgtgtagagc ttgtgctccg tgacgttgtc ccggtaggga gcttttatct
     8641 cggattcctt tatgttcgtc cggcgccact cctcgttgag atgtatcccg agtggcgcat
     8701 acggcagccc tctgtacgcc acgacctcgc ccccgtcgag gtccaggaag taccggctgc
     8761 tgccccacag atagacaggg gccagcgcta ttagcaccac gagcactatc gccagcgccc
     8821 gcgtcaggtt tcccgccgtg cgggtgaacc cttccctcgg cccttttctg cgtctcttgg
     8881 cagcgctctt ggcagcatcc cggcgctccc tgcgcgatgg agttttttca cggggcggct
     8941 ccggggacct cgtcacgtcc tccggggcgg agacggcctg catctcctgg gtgccggagc
     9001 gctcctcctg ggggctctgc ttcctgacgt ccacgacgat gacggtgatg ttgtccgtgc
     9061 cgccggcttc gagggccgct gtcagcaggc gccgggcggg tgtctccggc tcatccgggg
     9121 cggctatcag gatctcacgg atgcggtcct ccgggaccat gtccgagagc ccatcggagc
     9181 acaggattat ccggtcattc gccctgactg agagcgtcga ggtgtccacc tcaacatctt
     9241 cgtcggcccc gatggcgcgg gtgatgaggt tcctctgcgg atggttggac gcctgcgcgc
     9301 gggtcaggtc cccgctgcgg accagttccg cgacgagcga gtggtcctcg gtgaccggct
     9361 tgaggtctcc gtcgcgcagc aggtaggccc gcgagtcccc gacgtgggcg atctccgcga
     9421 ccgggtcccg ctgggtgccg gagaaccgcg ccgcgacgac ggtcgtgccc atcccggaga
     9481 gcctctcgtc gttgtgagcg gcttcgagga tgcggcggtt cgcctctcgg acggcctcct
     9541 cgaaagagtc tcccggctcc atctccctca gcacgtctac ggtgatggaa ctcgcgacct
     9601 ctccggcctc gaagccgccg atgccgtcgg cgacggcgaa gagcgtctcg tccttgccgt
     9661 ctcctacgag cagcgagtct tcattgttct tgcggacctt acccgcgtcc gtcacaccga
     9721 agtgttccag gaacagcatc gctccggctt attcctcgta cctgaaggtc gtggacccaa
     9781 ccttcacctt gtctcccgag cgcagcggcg tggctcccac ggtctttcgc ccgttgacga
     9841 aggtgccgtt cgtggaaccc acgtcctcca cgtacaggag gccgccgtgc cgggtgagca
     9901 tcgcatgttt cccggaggcg aagtcgtcgc tcttgagcac tatgtcgctg gcggaggagc
     9961 gcccgaggct cgtggtcccg ctgtctatcg ggaaacgggt gcgcggcgcg atgatggccg
    10021 agtcctcgac gatcaactcc gcgaccggat ctttccccgg tggcggcacg taagccccgc
    10081 gtccgttccg ccccgcgcct ctgctggagc cgggctcgta tgactcgtcg tccgggatgt
    10141 cgcgcaggtc cccgacgccg cggcgcacca cggcatagat gaacgcgaag agcagcaaga
    10201 gcagcagagc cttcgccgcg agaagcggca cctgcaactc cagcatctaa cggctcccgg
    10261 aggtccggtt ctgccgggac gtgactccac cgggttcgcc gggttgctct ccgccgggtc
    10321 ggcccccgcc ggggctgtcg ctgtcgcgac ggacgaagcg ggccgtgctc tggccaaacg
    10381 tcagctcgtc tccgttgtct atgcgctcgc ggtctatggg agcgccgtcc aggagcgtgc
    10441 cgttggtgga gccgaggtcc tcgattatga agccgttgtg ggctcgcagg agccgggcat
    10501 gcttgcggga gacgttcggg tcgttgatgg tgatgtcgtt ctcctcggag cgtccgatgc
    10561 tccatggacc cggtccgtcg agcgggtgcg gcttgccgtc cagcaccagt tcgaccacct
    10621 cccgcgccag cccttgcttc cgggcgtcct cggctgagat ggccgccgtc ccctcgatgt
    10681 tttgctcttc ctcgccggtg tccgtgcgga agatgcgcgt ctggcccgag gtgtcctggg
    10741 gcgcgccctt ctcgcggggg ccggagccgc cggtaagctt ggcggtgacg ccgaactcac
    10801 cgaagcgcag ggactcttcg gtctcgaagc gtatcctcgg gggactcatc agatggtagc
    10861 tcttgttctg cgcgtgggtg gaggcgtatt ggatcaactc gtcccggagc gagtccttgt
    10921 aggcctggat ggactccgtg tccgcctggg agaggtggat ggtaaagtcg ttgggcgcgt
    10981 aggtgcgcga tatcgagacc atccggttct catccatctg cttggtgagc cccttggcga
    11041 tctccacggg atggatctga cgccggaatg cccggccgaa gacgccctct acgagcgact
    11101 ccatcctctt ctctatctgc ttgaggaacc ccaaagagac tctaacctct cacgcgcgaa
    11161 ccgagatacc gtaccacccc gtatattata gacgccagac cgagggaagt catagcctcc
    11221 atcagggagt tgcgcgaggt cgagatgacg agcgcgtacg cgagcgctcc tcccccggcc
    11281 gcgagcacga gcccggtagc ccaccgtccc gtccattccg cgaccgagac caccccggcc
    11341 atcgcagccc agagcatcgc tagcgccgga agcggcggat acgcctgcag tatgtcctgc
    11401 cccctctgta tgagttccat cggggcgagc gcccccggca ctttctggaa cgggacgccg
    11461 aagtagggga gcactccgtc tccaatggtc aggtcgtagc agatgagcgt ggcggcgccc
    11521 atggccgcgg agacgcacgc gcccacggga cgcatgagat acccgaagag cagcggcaga
    11581 ccggctccga gcccgacgag ggcgagtggg atggcgagga cgggaccgag cggacaccgg
    11641 ctcgcgctcc ttgatgagcc ggtaccggcg atccagagcc ccccaagcat cgggacgagc
    11701 agcgagaacc cgagccccac cccatttttg aacagcagca ccgccagcgc gaccacgagc
    11761 cccaacgccg ccagacgcgg caggaggaag cccgcaacgc ccagacccgc cgcaatgcct
    11821 acggcctgcg cgccgttctg tagcaggagg tccgtgccga gctggtagcc gagccagctg
    11881 gccgccagtc cattcgccag cctgagcgcc tgctccggca gactgcccgc gacccggcgt
    11941 tcttctctgg ttggcttctt gttcggtttc ggggcgctcg ccttggaggg atacggagcg
    12001 ggggcgcggc gcccggtcag gagcttgagg gcggcccagg cgctctgcgg gcggtgagcc
    12061 gggttcgggg aggtcgcccg gtctatgaac tctctcaggc gcctgggagg ttcctgcggc
    12121 tggtaggcca gaagggtgcg ggcggtcgcg ccgacggcat agatatcgga gaggacgctc
    12181 gggtcggctc cgtccatgat ctccggcgcg atatatcccg gcgtcccgac ggcgtagccg
    12241 atgcgagtca ggcgcgtgtc gccagcccgg taggcgaccc cgaagtccgt cagcttcacg
    12301 cggccccggc cgtccacgag ggcgttctgg ggctttatgt cacggtggat gatgccctgg
    12361 gcatgggcgt agaccagggc ttcgaggacc tgggccaggg cgttgatgac ctggttcacg
    12421 tcgtagtcgc gggcggcctc gtccaggggc aggccccgga cgtactcggt gaccaggtag
    12481 atctcggtgt ccccggggat cacctctatg gtctcgacga tgttcttgtg gcgcagaccc
    12541 tcggcgatct gaccctcccg catcgcccgc gagcgttcct gggaagagta gatagggatg
    12601 atcttgatcg ccacgtcatt gccgcgggac ccgaaggtga aggagccgcc gaaggagcga
    12661 gcccgccaca cggtggcgaa cccgccgcga cctaccatct cggccagagc gtatcggccc
    12721 tggccctcga tgtagcgtgt ctcggccatg aatacaagct attgtatagc cttgcgcaac
    12781 aactttaacg atccgggcca tcaggcgtta gcgacgagcg gcgttgcttg gtacaatccc
    12841 tcaaggtttc agacggttcc tgaaggaagt cctgaaggag ataccggagg gttcctacgg
    12901 gcgagtggcg gaattggtag acgcgctagg ttcagggtct agtgtccgag aggacgtggg
    12961 ggttcgagtc ccccctcgcc cacagtgtag acgtaggggg tttcgataga tcgcagagcc
    13021 cagagacccg cctccaggag tcggcgcagc cgcccgtctc cggcggcaaa gaggcgcccc
    13081 agccagcggc gcgagcgggc gctccccccg tggctgacgc ttctgggttc gatctttctc
    13141 ctgctcgccg ccgtcgcttt tatctttttt atcgcccgcg gctgcgttgc tacccaggaa
    13201 tccacccagg tgcgcaagta cgtcaccagc tccgctagcc tgctgagcga ttcttccaac
    13261 gccggcaagc aggacctcca gaacacgctc gccaacgccg gcggcgacgc cagcaagctc
    13321 gacggcgagg ctctcagaaa cgcttcggac aacgcgcaac tcctctacca gcgggcgctg
    13381 gacaacgacg aagtgccccc ggagtt
//
LOCUS       DASSMB010000170         8134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_546447, whole genome shotgun sequence.
ACCESSION   DASSMB010000170 DASSMB010000000
VERSION     DASSMB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8134
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546447"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /gene="tuf"
                     /locus_tag="VFJ72_10080"
     CDS             <1..151
                     /gene="tuf"
                     /locus_tag="VFJ72_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7479845.1"
                     /translation="EGVEMVMPGDNTVMNVELISPIAMDEGLNFAIREGGRTVGAGVV
                     TEIVE"
     gene            159..470
                     /gene="rpsJ"
                     /locus_tag="VFJ72_10085"
     CDS             159..470
                     /gene="rpsJ"
                     /locus_tag="VFJ72_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7479846.1"
                     /translation="MAVSKIRIKLKAYDHEVIDKTAKSIVETAERTGAFVFGPVPLPT
                     KKSRYTVIRGPFKDKDSREHFQLHTHKRLIDIQQPTPRTVDSLQRLDLPAGVNIEIKA
                     N"
     gene            474..1091
                     /gene="rplC"
                     /locus_tag="VFJ72_10090"
     CDS             474..1091
                     /gene="rplC"
                     /locus_tag="VFJ72_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7479847.1"
                     /translation="MATAVFGRKRHMTQAFQDDGTLVSVTVIEVEPNFITAVRTADED
                     GYDAVQVGFGTVPSRKVNRPHAGVFEKHEAPARKHLRELRGVTGEAGESIGADVFEVG
                     DKVHVTATSKGKGFQGTVKRHGFGRGPVSHGSHNVRAPGSVGASADPARIFKGQKMPG
                     QMGNKNATVKNLQVVNIDTEKNELWVRGGVPGGKGAVVKIRKAGE"
     gene            1093..1722
                     /gene="rplD"
                     /locus_tag="VFJ72_10095"
     CDS             1093..1722
                     /gene="rplD"
                     /locus_tag="VFJ72_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012782.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET7479848.1"
                     /translation="MAQAQVFDARSGNKSSLDLKGPKFETEPREHVIHRAVVAELDSR
                     RRYTASTKGRSDIQGSGRKLYRQKGTGRARAGDIKSPVRVGGGTWGGPKPKGERSRKR
                     INRKEALAAFHGVLSAKAADGELFVLDSLAFDKPSTRQAKDLLGKMDLQGPVLVVLTD
                     DDEHAALSFRNLPKVTVVGSYEYGVYELLRAREVVFSRAAYSRLAGEEK"
     gene            1724..2011
                     /gene="rplW"
                     /locus_tag="VFJ72_10100"
     CDS             1724..2011
                     /gene="rplW"
                     /locus_tag="VFJ72_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7479849.1"
                     /translation="MDPHQVIIRPVISEKSYNLIEGEGQYTFQVDRRANKNQIKNAIE
                     GAFDVSVAKVNTVNVKSKPKRQGLTRGRTAVWKKAVVKLAPGDRIELFEGV"
     gene            2015..2854
                     /gene="rplB"
                     /locus_tag="VFJ72_10105"
     CDS             2015..2854
                     /gene="rplB"
                     /locus_tag="VFJ72_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7479850.1"
                     /translation="MPARRYKPTSAGRRNSSVLTRDSVTKNKPEKKLTKKLNKTGGRN
                     NLGRITTRHIGGGHKRRYRMIDFKRRKDGVPATVAAFEYDPNRSANIALLHYHDGEKR
                     YILAPRNLTVGSLVMSGPDAEVDVGNTLPLNRIPVGTVVHAVELEPGRGAQMARSAGT
                     SAQLVAREGALVTLRLPSGERRRVRAECRATVGVVGNQSHQNVRWGKAGRKRHLGIRP
                     TVRGTVMNPVDHPHGGGEGKSTPGRAPVTPWGQITQGSPTRKKHKRSDAMIVRRRRKG
                     RRR"
     gene            2855..3136
                     /gene="rpsS"
                     /locus_tag="VFJ72_10110"
     CDS             2855..3136
                     /gene="rpsS"
                     /locus_tag="VFJ72_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7479851.1"
                     /translation="MTRSSKKDPFVEERLMERILRMNENNEKRMLKTWSRASVIYPEF
                     VGHTIAVHDGRKHVPVYCTESMVGHKLGEFAPTRTFRTHRKDDRTARRR"
     gene            3136..3501
                     /gene="rplV"
                     /locus_tag="VFJ72_10115"
     CDS             3136..3501
                     /gene="rplV"
                     /locus_tag="VFJ72_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET7479852.1"
                     /translation="MKATAKYLRIAPRKARLVADAVRGKSIPEAVSILQFTNKAAAKI
                     VGDVVKSAAANAEHNLDADPDELFVRDIRVDDGPTIKRYRARAMGRATMIRKRTSHIT
                     VELGAPAGVLVGTPGDEEE"
     gene            3502..4176
                     /gene="rpsC"
                     /locus_tag="VFJ72_10120"
     CDS             3502..4176
                     /gene="rpsC"
                     /locus_tag="VFJ72_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET7479853.1"
                     /translation="MGQKVHPEGFRLGVKRKGEKVDFKSSWYSDKDFAELLGEDLKIR
                     AHIEKKLTRAGISDIQIRKAAEQGEIAVDIHTARPGIVIGKGGSEVDALRGELERLTR
                     KKVQVNVREVSRPELNAALVAESIAEQLEGRAAFRRTMKRALTSAMRSGAEGCRIRCG
                     GRLGGIEMSREETISEGRVPLHTLDADIDYGFREAKTQMGQIGVKVWINHGVHEDGGS
                     AGGGGR"
     gene            4180..4596
                     /gene="rplP"
                     /locus_tag="VFJ72_10125"
     CDS             4180..4596
                     /gene="rplP"
                     /locus_tag="VFJ72_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET7479854.1"
                     /translation="MLVPKKLKYRKPHRGRMRGLAKGGTEIEFGDYGLQALEASWITN
                     RQIEAARIAMTRKIKRGGKVWINIFPHKPVTKKPAETRMGSGKGSPEGYVAVVKPGRV
                     MFEMSGVDEALAREAMNLAAQKLPIKTRFLIRGGGQ"
     gene            4596..4790
                     /gene="rpmC"
                     /locus_tag="VFJ72_10130"
     CDS             4596..4790
                     /gene="rpmC"
                     /locus_tag="VFJ72_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET7479855.1"
                     /translation="MKVAEVRELDVQELEDRIAATRRELFNLRFQHATGQLENTGQLK
                     EVRKNIARLLTVLNQKKQEN"
     gene            4992..5258
                     /gene="rpsQ"
                     /locus_tag="VFJ72_10135"
     CDS             4992..5258
                     /gene="rpsQ"
                     /locus_tag="VFJ72_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET7479856.1"
                     /translation="MQTDTERNRRKERVGLVVSDKSDKTVTVAVETLVRHKRYKKRVR
                     STKKFMAHDERNEAHIGDTVRIVETRPLSARKRWRLGSVISRAE"
     gene            5258..5626
                     /gene="rplN"
                     /locus_tag="VFJ72_10140"
     CDS             5258..5626
                     /gene="rplN"
                     /locus_tag="VFJ72_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET7479857.1"
                     /translation="MIQNETRLKVADNSGARSILTIRILGGSKRRSAGIGDVIVATVK
                     EATPGGTVKKGEVVRAVVVRTAKETRRDDGSYIRFGENAGVIINPDGAPRGTRIFGPV
                     ARELRDKGYTRIISLAPEVL"
     gene            5629..5946
                     /gene="rplX"
                     /locus_tag="VFJ72_10145"
     CDS             5629..5946
                     /gene="rplX"
                     /locus_tag="VFJ72_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET7479858.1"
                     /translation="MGLKIKRGDTVVVISGKEKGKRGEVERVIPAKNRVVVGGVNVRT
                     RHARPSQNNQQGLFTFEAPIHISNVMLVDPNSGEPTKVKYRFTDSGEKLRVGAKSGKD
                     IDG"
     gene            5939..6481
                     /gene="rplE"
                     /locus_tag="VFJ72_10150"
     CDS             5939..6481
                     /gene="rplE"
                     /locus_tag="VFJ72_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET7479859.1"
                     /translation="MARLRDRYREEVAPALKEKFGIENPMRIPKLEKIVVNMGVGEAV
                     VNSKAMDGAMEDLAKITGQKPQLRRARKSIAGFKIREGMPVGARVTLRGDRMWEFLDR
                     LVSIALPRTRDFRGISPNSFDGRGNFALGLREQLIFPEISYDSVDATRGLDVAVVTTA
                     ETDEEARELLRLLGMPFRQS"
     gene            6492..6677
                     /locus_tag="VFJ72_10155"
     CDS             6492..6677
                     /locus_tag="VFJ72_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HET7479860.1"
                     /translation="MTRKALLVKQQKQQKFGVREYSRCQRCGRSRGYYRKFGLCRICL
                     RELAHEGKIPGVTKASW"
     gene            6682..7080
                     /gene="rpsH"
                     /locus_tag="VFJ72_10160"
     CDS             6682..7080
                     /gene="rpsH"
                     /locus_tag="VFJ72_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET7479861.1"
                     /translation="MSVNDPIADFLTRIRNAQMAKHETAEMPHSRMKVELARILKEQG
                     YINDFSEKGAGAEARLVIELKYGPDGRRGITGLRRMSRPGRRVYRKQNDIPRVLDGLG
                     VAILSTSRGILTDHAARRQGIGGEVLCFVY"
     gene            7096..7629
                     /gene="rplF"
                     /locus_tag="VFJ72_10165"
     CDS             7096..7629
                     /gene="rplF"
                     /locus_tag="VFJ72_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET7479862.1"
                     /translation="MSRIGKAPVELPGGVNAEVSVRSVKVTGPKGELTVPVGRGVKVN
                     QDDGSLVVERASDAPEHRAMHGLTRSLLQNAVTGVTDGFTRTLNIAGVGYRAQLQGRD
                     INLQVGYSHPVSVTPREGIEFEVPSPTVIIVRGIDKQRVGQTAAEIRKVRPPEPYKGK
                     GIRYSDEQIRRKVGKAG"
     gene            7640..7990
                     /gene="rplR"
                     /locus_tag="VFJ72_10170"
     CDS             7640..7990
                     /gene="rplR"
                     /locus_tag="VFJ72_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET7479863.1"
                     /translation="MAVLQKRAHRETRRKRVRRKIAGTAERPRLSVYRSNVNIYAQLI
                     DDDAGHTLASADSREVGDAENRKDAARKVGELIARKASDAGIETVVFDRGGNRYHGRI
                     AALAEGAREGGLKL"
BASE COUNT         1693 a         2323 c         2930 g         1188 t
ORIGIN      
        1 agaaggagta gagatggtga tgcccggaga caacacggta atgaacgtgg agctcatctc
       61 gcccatagct atggatgagg gtctcaactt cgcgatacgt gagggtggca ggactgttgg
      121 tgctggtgtc gtcaccgaga tcgtcgagta ggtgactgat ggccgtctcc aagataagga
      181 taaagctgaa agcctacgac catgaggtca tagacaagac ggctaagtcc atagtggaaa
      241 cggcggagcg gaccggggcc tttgtgtttg gccccgtccc tctgccgacg aagaagagcc
      301 gctacaccgt gatccggggt cccttcaagg acaaggactc gcgggagcac ttccagcttc
      361 acacgcacaa gcggctcata gacatccagc agcccacgcc gcgcacggtg gattcgctac
      421 agcgtctcga cctgccggcg ggcgtgaaca tcgagatcaa ggcgaattag gttatggcaa
      481 cggcagtttt cggacgcaag agacatatga cgcaggcgtt ccaggacgac gggacgctgg
      541 tgagcgtgac cgtgatcgag gtggagccga acttcatcac cgccgtccgc accgccgacg
      601 aggacggcta cgacgccgtt caggtcggtt tcgggaccgt tccctcgcgc aaggtcaatc
      661 ggcctcacgc gggcgtcttc gagaagcacg aggcgccggc ccgcaagcac ttgagggagc
      721 ttcgcggggt tacgggtgag gccggcgaga gcattggggc ggacgtgttc gaggtgggcg
      781 acaaggtcca cgtgaccgct acctccaagg gcaagggctt ccaggggacc gtcaagcgcc
      841 acggcttcgg ccggggtccg gtctcgcacg gctcgcataa cgtgcgggcc cccggctcgg
      901 tcggcgcctc ggctgacccg gccaggatct tcaagggcca gaagatgccc ggacagatgg
      961 gcaacaagaa cgccacggtg aagaaccttc aggtcgtcaa tatcgacacg gagaagaacg
     1021 agctctgggt gcgcggtgga gttccgggcg gtaagggcgc tgtggttaag atccggaagg
     1081 cgggtgagta ggatggcgca ggcgcaggta ttcgacgccc gcagcggcaa caagtcttcg
     1141 ctggacctga aaggcccgaa gttcgagacc gagccccggg agcacgtgat acaccgggcc
     1201 gttgtcgcgg agttggactc tcggcggcgc tacacggcct cgaccaaggg tcgtagcgat
     1261 atacagggct ccgggcgcaa gctgtatcgc cagaagggca ccggccgggc gcgggcgggc
     1321 gatatcaagt cgccggttcg ggttgggggc ggcacctggg gcggtccgaa gccgaagggc
     1381 gagcggtcgc gcaagcgcat caaccgcaag gaggccctcg cggccttcca cggcgttctc
     1441 tcggcgaagg cggcggacgg cgagttgttc gttctcgact ccctggcttt cgacaagccg
     1501 tctaccaggc aggcgaagga tctactcggg aagatggatc tccagggtcc ggtcctggtc
     1561 gttcttacgg acgacgacga gcacgcggcg ctgtcgttca ggaacctgcc taaggtcacg
     1621 gtcgtcggga gttacgagta cggggtctac gagctcttgc gggctcggga ggtcgtgttc
     1681 tcgcgggcgg cttattcgag gctcgcgggg gaggagaagt agtatggacc cgcatcaggt
     1741 gataatccgg ccggtcatct cggagaagag ctacaacctc atcgaaggtg agggacagta
     1801 cacgtttcag gtggatcggc gggcgaacaa gaaccagatc aagaacgcca tcgagggcgc
     1861 tttcgacgtt agcgtcgcga aggtgaacac ggtaaacgta aagagcaagc ccaagcgcca
     1921 gggcctcacg cggggccgca cggccgtgtg gaagaaggcc gtggtaaagc tggcgccggg
     1981 cgaccgcatc gaactgttcg agggggtcta acagatgcct gcgagacgtt ataagccgac
     2041 gagcgcgggc cgcaggaact cctcggtcct cacgcgagac tcggttacga agaacaagcc
     2101 ggagaagaag cttacaaaga agttgaacaa gaccggcggc cggaacaacc tcggccgcat
     2161 cacgacccgt cacattggcg gcggtcacaa gcggcgctac cgcatgatcg acttcaagcg
     2221 ccgcaaggac ggtgtgccgg cgacggtagc agccttcgag tacgacccga accgctcggc
     2281 gaacatcgcc ctgctccact accacgacgg cgagaagcgc tacatactcg cgccgcgcaa
     2341 ccttacggtt ggctcgctgg tcatgagcgg cccggacgcc gaggtagacg tgggcaacac
     2401 gttgccgctg aaccgcatac cggtcggtac cgtggtacat gccgtggagc ttgagccggg
     2461 acgcggagcg cagatggcgc ggagcgccgg gacgagcgcg cagctagtcg cccgcgaggg
     2521 cgcgctggtc acgctgcgtc tgccgagcgg cgagcgccgt agggtacgag cggagtgtcg
     2581 ggctaccgtc ggcgtcgtcg ggaaccagtc gcaccagaac gtgcgctggg gcaaggcggg
     2641 ccgcaagcgg cacctgggga tccgtcctac ggtgcgcggt acggtcatga acccggtgga
     2701 ccacccgcat ggtggcggcg agggcaagtc tacgccgggc cgggctccgg tcacgccgtg
     2761 ggggcagatc acccagggtt cgccaaccag gaagaagcac aagcggtcgg acgccatgat
     2821 agtgcgccgg cgcaggaaag gaaggaggcg ctagatgacg cggtcctcta agaaggatcc
     2881 attcgtagag gagcgcctta tggagcgcat cctccggatg aacgagaaca acgagaagcg
     2941 gatgctcaag acgtggagcc gggctagcgt gatctacccc gagttcgtcg gtcacaccat
     3001 cgccgtccat gacgggcgca agcacgtccc cgtctactgc acggagtcga tggtcggcca
     3061 caagctcggc gagttcgccc cgacccgcac cttccggacg caccgcaagg acgaccggac
     3121 cgcgaggagg cgatagtgaa ggcgaccgcc aagtacctcc gtatcgcgcc ccgcaaggcg
     3181 cggctggtcg cggacgccgt ccgggggaag tccatcccgg aggcagtctc gatcctgcag
     3241 ttcaccaaca aggccgcggc gaagatcgtc ggcgacgtgg tgaagagcgc cgccgccaac
     3301 gcggagcaca acctcgacgc ggatcccgac gagctattcg tccgggacat ccgggtggac
     3361 gacgggccga ccataaagcg gtatcgcgct cgcgcgatgg gtcgggcgac catgatccgc
     3421 aagcgcacca gccatataac cgtcgagctt ggcgctccgg caggagttct tgtgggcacg
     3481 cccggagacg aggaggagta gatgggccag aaagtacatc ccgaaggctt ccgcctgggc
     3541 gtcaagcgca agggcgagaa ggtagatttc aagagtagct ggtactcgga caaggacttc
     3601 gccgagttgt tgggcgagga cctcaagatc cgggctcaca tagagaagaa gctcacgcgg
     3661 gccggcatct cggacatcca gatccggaag gccgccgagc agggcgagat cgcggttgac
     3721 atccataccg ctcgtcccgg catcgtcatc ggcaagggcg gctcggaggt ggatgccctg
     3781 cgcggcgagc tggagcgcct caccaggaag aaggtgcagg tcaacgtgcg cgaggtgtcg
     3841 cggccggagt tgaacgccgc gctcgtcgcc gagtccatcg cggagcagct agagggtcgg
     3901 gctgctttcc ggcgcaccat gaagcgggcg ctcacgagcg ccatgcggag tggagccgag
     3961 ggctgtcgca tacggtgcgg cggacgattg ggcggcatcg agatgagccg ggaggagaca
     4021 atctccgagg gccgggtgcc gttgcacacg ctggacgcgg acatagatta cgggttccgc
     4081 gaggcgaaga cgcagatggg ccagatcggc gtgaaggtct ggatcaacca cggcgtccac
     4141 gaagacggtg gttccgccgg aggcggtggg aggtaaacga tgctcgtacc gaaaaaactc
     4201 aagtaccgca agccccaccg gggccggatg cgggggctcg ccaagggcgg cacggagatc
     4261 gagttcggcg actacgggtt gcaggcgctg gaggcgtcct ggatcaccaa tcgccagata
     4321 gaggcggccc gtatcgcgat gaccaggaag ataaagcgcg gagggaaggt ctggatcaac
     4381 atcttccccc acaagcccgt gaccaagaag ccggccgaga cccgcatggg ttccggcaag
     4441 ggtagccccg agggctatgt cgcggtcgtc aagccgggcc gcgtgatgtt cgagatgagc
     4501 ggcgtggatg aggcgctggc gcgtgaggct atgaacctcg cggcccagaa gctcccgatc
     4561 aagacccgct tcctcatccg tggaggtggt cagtaatgaa agtcgccgag gtccgcgagc
     4621 tggacgtgca ggagttggag gaccgcatcg ccgccacaag gcgcgagctg ttcaacctgc
     4681 gtttccagca cgctaccgga cagttggaga acacgggaca gctcaaggag gtccggaaga
     4741 acatcgcacg gctccttacc gtcctgaacc agaagaagca ggagaactag cgtatggcag
     4801 acgagagaga tacccggacg ccggacgagg cgagggagcc tgccgagagc aaggctcccg
     4861 agaccgaggc tcccgaagag gaagccgtag agacgttcgc cgacaccgaa gaggtggcga
     4921 cgaccgaggt tgccgaagac ctcggcgcga gcgccgcgaa gggaccggcg ttcgaccagg
     4981 acgagggaca gatccagacc gacaccgagc gcaaccggcg caaggagcgg gtcgggctgg
     5041 tcgtctccga caagtcggac aagaccgtga ccgtcgcggt cgagacgctg gtgcggcaca
     5101 agcggtacaa gaagcgcgtg cggagcacca agaagttcat ggcgcacgac gagcgcaacg
     5161 aggcgcacat tggggacacg gtgaggatcg tcgagacccg gccgctctcg gcgcgcaagc
     5221 ggtggcgtct gggcagtgtc atatcgaggg ccgagtaatg atccagaacg aaaccagact
     5281 gaaggtcgcg gacaactccg gcgcccggag tatcctgacc atccgcatcc tcggcggctc
     5341 caagcgccgc agcgcgggga tcggggacgt catcgtcgcc acggtcaagg aggcgacgcc
     5401 gggcggtacg gtgaagaagg gcgaggtcgt gcgggccgtc gtggtgcgaa ccgcgaagga
     5461 gacgcgccgg gacgacggtt cctatatccg gttcggagag aacgcgggcg tcatcatcaa
     5521 cccggacggc gctccgcgcg ggacccgtat cttcgggccg gtggcgcgcg agctacgcga
     5581 caagggctat acgaggatca tctcgctggc tccggaggtg ctctaactat gggactcaag
     5641 ataaagcgcg gggacaccgt tgtggtgatc tccggtaagg agaaaggcaa gcgcggcgag
     5701 gtcgagcggg tcatcccggc gaagaaccgg gtcgtcgtgg gcggagtcaa cgtccgcacc
     5761 cggcacgcga ggccgagcca gaacaaccag cagggactgt tcaccttcga ggctccgatt
     5821 cacatctcga acgtgatgct ggtggacccg aactccggag agccgacgaa ggtgaagtac
     5881 cgcttcaccg actcgggcga gaagcttcgg gttggggcga agtccgggaa ggatatcgat
     5941 ggctaggttg agagacaggt accgggaaga agtcgctccg gcgctcaagg agaagttcgg
     6001 catcgagaac ccgatgcgga taccgaagct ggagaagatc gtggtgaaca tgggggtcgg
     6061 cgaggccgtg gtcaacagca aggcgatgga cggcgcgatg gaggacctcg ccaagatcac
     6121 cggccagaag ccgcagctaa ggcgggctcg caagagcatc gcggggttca agatccgtga
     6181 gggcatgcct gtcggcgcga gggttacgct gcggggcgac cggatgtggg agttcctgga
     6241 ccggctggtc tcgatagccc tgccgcggac gcgggacttc cgggggatca gcccgaactc
     6301 cttcgacggg cgcggcaact tcgcgctggg cttgcgcgag cagctaattt tcccggagat
     6361 cagctacgac tccgtggacg ccacgcgcgg tctggacgtg gcggttgtga cgacggcgga
     6421 gaccgacgaa gaggcccggg agcttttgcg gctgctcggt atgccgttcc ggcagagtta
     6481 aggggttatt gatgacgagg aaagcgttgc tcgtaaagca gcaaaagcag cagaagttcg
     6541 gggtgcggga gtacagtcgc tgccagcggt gcggccgttc ccgcgggtac tacaggaagt
     6601 tcggcctctg caggatctgc ctgcgggagc tggcgcacga ggggaagatc cccggcgtaa
     6661 cgaaagcgag ttggtagcaa gatgtcggtt aacgatccaa tagcggattt cctcacccgt
     6721 atccgcaatg cacagatggc gaagcacgag acggccgaga tgccgcactc gaggatgaag
     6781 gtcgagttgg ctcgtatcct gaaggagcag ggctacatca acgacttctc cgagaagggc
     6841 gccggggccg aggcgagact cgttatagag ctgaagtacg gcccggatgg acgccgcggc
     6901 atcaccggcc tgcggcgtat gagccgcccc ggacgccggg tgtaccggaa gcagaacgac
     6961 atcccgcgcg tcctggacgg gctgggtgtg gctatcctct ctacctcgcg gggaattctc
     7021 accgaccatg ccgctcgccg gcagggtatc ggcggcgaag tcctctgctt cgtgtactag
     7081 gaggtttcga ggaatatgtc tagaatcgga aaagcgccgg tcgagttgcc gggtggagtg
     7141 aacgccgaag tctcggtccg aagcgtgaaa gttacggggc cgaagggcga gctgaccgtg
     7201 ccggtgggcc gcggcgtcaa ggtaaatcag gacgacggaa gtctcgtcgt cgagcgggcg
     7261 tcggacgcgc cggagcaccg ggcgatgcac gggctcacgc ggtctctgct ccagaatgcc
     7321 gtaacgggcg tgacggacgg gttcacgcgg acgctgaaca tcgcgggtgt cggttatagg
     7381 gcgcagttac agggccggga catcaacctg caggttgggt actcgcaccc tgtctcggta
     7441 acgccgcgcg aggggataga attcgaggtc ccgagcccta cggtgatcat cgtgcggggc
     7501 atagacaagc agcgggttgg tcagacggcg gctgagatcc gcaaggtccg gccaccggag
     7561 ccgtacaagg gcaagggcat ccgctacagc gacgagcaga tccggcgcaa ggtcggcaag
     7621 gccggctaag ggaggaagta tggctgttct acagaagaga gcgcacaggg agacgcggcg
     7681 caagcgcgtc cggcgcaaga tcgccgggac cgcggagcgt ccgcgcctct cggtctaccg
     7741 ctcgaacgtc aatatctacg cccagctcat cgacgacgac gcgggtcaca cgctggcgtc
     7801 agcggactcc cgagaagtgg gagatgcgga gaaccgcaag gatgccgccc gcaaggtcgg
     7861 agagttgatc gctcgcaagg cgtccgacgc cgggatagag acggtcgttt tcgaccgggg
     7921 cggaaacagg taccacggcc gcatcgcggc gctcgcagag ggcgcgcgcg agggcgggct
     7981 gaaactctaa gagaactcta agagatttag gaggaacatc ttgggtcgtc caaggaacga
     8041 taacagaggc ggagcgccgg gtggtccggg tggccgcggc ggtggtccgg gtggccgtgg
     8101 aggcggtccc ggtggtcagg gcggtcgcgg cgga
//
LOCUS       DASSMB010000171        11115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1814398, whole genome shotgun sequence.
ACCESSION   DASSMB010000171 DASSMB010000000
VERSION     DASSMB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 11115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11115
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814398"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VFJ72_10175"
     CDS             complement(<1..354)
                     /locus_tag="VFJ72_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7479864.1"
                     /translation="MARKYEQKRRAEKQEETRRRITEATVELHKTVGMARTTISAIAE
                     NAGVERLTVYRHFPDEKTLFSACSEHYMGANPPPDPEPWARTGDPEERLRSALEEVYA
                     YHRRTEPMMANLIRDA"
     gene            543..995
                     /locus_tag="VFJ72_10180"
     CDS             543..995
                     /locus_tag="VFJ72_10180"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7479865.1"
                     /translation="MIAERISELELMEVWYEEDPTMRIRVTFPFFLGNGTRSTAVVYF
                     EIDPGHRLGTHTDSAEEIILILEGTVEVSLGDEAGRLSAGEMALVPAMAPHGMRNAGD
                     ETVRVVGFFSSNVVVSTFDRPMMPFGQRAVGTPPVLEEEQVATGDSGA"
     gene            1009..1260
                     /locus_tag="VFJ72_10185"
     CDS             1009..1260
                     /locus_tag="VFJ72_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479866.1"
                     /translation="MARIQTVPADEARGLRRFMFRGIQHQYGGVVPGIFEILMPDLQV
                     AFASGWLYNRLHMRKSSPMTRLQREMLATVVNGLVGGAP"
     gene            1282..1596
                     /locus_tag="VFJ72_10190"
     CDS             1282..1596
                     /locus_tag="VFJ72_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479867.1"
                     /translation="MRRLTKDEHLSAEFATTWPMYDLDEKTRALLAYAKKLTEEPGMI
                     GDEDIEDLRAAGWDERGIYEATALISFFNFTGRMEAASGLPPDDIPKGASFAEAVSDG
                     KR"
     gene            1726..2157
                     /locus_tag="VFJ72_10195"
     CDS             1726..2157
                     /locus_tag="VFJ72_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479868.1"
                     /translation="MKVLIVTVIIAAPAMVLGRVIWPPAEGGPEPSGAQLPFFLFLAL
                     MESILLGLGISFLAFGLPAVRRVSADSGLRAWVMYLAIGWLMVSWWPHDNMHIHNGEN
                     MQGLLYIEYGFHLTLMLTGIILAYCFLSILREGSGKPAVVR"
     gene            2212..2751
                     /locus_tag="VFJ72_10200"
     CDS             2212..2751
                     /locus_tag="VFJ72_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7479869.1"
                     /translation="MIENPVQGDRIVFRETSQDTGGELLRMEVVAFPQASGPVDHIHL
                     RQEERFEILSGTLNARLNNQEQSFGPGETLTIPPGTPHSWWNGGTEEARVLCELRPAL
                     NTETFFETIYGLARDGKTNKEGVPNLLQIAAMSRLHRDEIYLARPPIAVQRVLFALLR
                     PVARLLGYRSSYPEYSGSL"
     gene            2877..3269
                     /locus_tag="VFJ72_10205"
     CDS             2877..3269
                     /locus_tag="VFJ72_10205"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET7479870.1"
                     /translation="MRVRGDRAYVSGHGPLNPDGSLSGPFGKVGAEVSPEEGYEAARG
                     AALSVLASLKRELGDLDRVSAWLVVHGLVNAAPDFGGTAGVINGFSDLILDIYGPEAG
                     MHARSAPGVATLPLNQPVFVAAEVEVRP"
     gene            complement(3333..7313)
                     /locus_tag="VFJ72_10210"
     CDS             complement(3333..7313)
                     /locus_tag="VFJ72_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HET7479871.1"
                     /translation="MTTDYRPYLSRGGGATKKLLVALLVTLVTLTVWIPRALAQTAPT
                     INNPPVAPHSIIAFPQRDFVSASGYAATDKVTVSVVHKDGTEVGRVTGIVPQDDPQTP
                     EFDGLVEVNHPGGACWVGVTPDIRAGDKVRITVEGSGVADETTVADVTAKRPVQTAPD
                     TVQVHGTAQDAAGNPLPLDQLEQRLVVPNNQFALNGKRTLRAPGNGDISYDASGSINW
                     TATYTGLSAADVTKALGAESRGMWLGSNPLAAVESTIFENGAGAIPGPAAPCTAPLEK
                     LPPPPGSELEPPTAPSGLSATANLSSVTLDWTAATDNVGVTSYGIYRNGVAIANVQKP
                     DGTAPAPTTFVDKNLPAGDYTYTVDAADAVGNRGPLSNEVTVTTTVNPAPITAVNEPP
                     VLPHSIIGFPQRDFVSAQGYAPDDIVDVFLIRDGAIIGSATGLIPQDDERTDGFDGLV
                     EVNHPGGGCWEGTTPWMMPGDQIRLVTNKGDRDQTTVSNVTAKRPVQTGPDSIAIHGT
                     AQDAKGNPIDLGQVEERLVANRDAFDLSGKRTLRAPGNGDVSYDVPGSINWTATYKGI
                     DAADMDRALNRAESRVLWLGHDPLAGAELTIYEVGTTAVPVDKGPSAPCTAPTAFKPP
                     APAADTTAPDAPTNLDATVLGGNDVKLTWTKSSSADVIGYGVYRDDKLVGEVRGDRLA
                     DSPLTFTDSGVAQGTYNYTVKAFDAADNHSAASNSVTANTVDVPPAPGAVNEPPKSPH
                     SIISFPQRDFVSATGYAQTDQVRVDIVRGGNVVSSTPDITPQDDPGTPGFDGLVEVNH
                     PGGGCWNVNTPDIRPGDKVRLTVTNSGALPDQTTSYDVTAQRPVQTASDTVVIHGTAV
                     DPATGGPVDIGQLEQRLVSGKDLFDLNGKRTLRAGAGAEGTLTYDAPGSTKWTATYKG
                     LSAADVTRALAAESRAVWLGHAPLVGNELTIYENGPGVVGGPSTPDCTAPLEPSAPQA
                     SATPASLNFGNQSAVPATTSTPKNITLSNSGTSDVKVSDTYIAGANPGDFAIGSKTCP
                     ATLAAGASCTVNVTFSPKAVGARTASLNLRTNAANQGDISTSLTGNGINAAAPSAPGA
                     PAQSLPTVSQLLVDNAALANSKIPVKLNWTASTGTVTSYQLQQSTNGGAFTDVTPQPG
                     TASSTTLNLAMGTTAAPSSYQFQVRACNGADCSAWVSGPNFKLAPIDDSNRANLSYGG
                     TWTTQALAGAYGGSVSFASTAKDKVQFNKLTYTVPGSSIAWVSTLGPDRGKATVTVDG
                     GPAQQVDLYSATRQPAKVVFAANNLNGPAEHTITVQILGTKNASSTGTRVDMDAIVAL
                     GATPKQ"
     gene            complement(7709..8818)
                     /locus_tag="VFJ72_10215"
     CDS             complement(7709..8818)
                     /locus_tag="VFJ72_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7479872.1"
                     /translation="MAVDVEERTFSEVKRLCYAGLDGPVLLDEVIKRLRRAVPFEAYC
                     ASTMDPASGLLTHPISDEMGGAKEAAIFFERIYFEHDLKLYKQMAQSHRAVARLSETA
                     GGRLERSLRYLELLGPLGLAHEMRGVFGAGGYLWGSMDLIREKGRPDYGPRQVALLRR
                     IAPHLGSGLKTAALRTQAPVNPTGADVPGVLTIDGSGRVVQHTAAAERWLRELEDLGP
                     GWREWAGLPPTLRMAMVVLRRALRPERERDTESIPRLRARTRSGRWLTLYGSLTEATP
                     ERRAETVIVIEPTKPEELLPFSMISYGLSAREEEVVGLIVRGLSTAQISRTLYISEYT
                     VQNHLRNVFEKVGVRSRGELVRRLFFDNLYPTLFE"
     gene            9015..9500
                     /locus_tag="VFJ72_10220"
     CDS             9015..9500
                     /locus_tag="VFJ72_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7479873.1"
                     /translation="METTEHAVTHSPDGEGKTVWMVGTDLITFKATGEDTEGAFALFD
                     SLVLPGGGPPPHIHHRESESFYVLEGRFEFLAENGWIKAAPGSFVHVPKGTLHTLKNA
                     GEGVGRLLTLAMPAGLDRFFEEAGEPGTDVSTPPGPPDVEKLLATAERYGIEFPPPPA
                     Q"
     gene            complement(9530..10036)
                     /locus_tag="VFJ72_10225"
     CDS             complement(9530..10036)
                     /locus_tag="VFJ72_10225"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET7479874.1"
                     /translation="MRTLTIVVIAALLLLAGCGGGPGDSSRGADTTKASAGETTGPTS
                     GLRTVTIDASGGEKVKIRVEIADTPAEQEKGLMFRKSLGKDRGMLFVFPDEQVRSFWM
                     KNTLIPLSIAYIDSDGRIIDLQEMKALDDQPPHYVSAGPARYALEANKGYFEEHGVKV
                     GARAELPE"
     gene            complement(10033..10743)
                     /gene="alaXM"
                     /locus_tag="VFJ72_10230"
     CDS             complement(10033..10743)
                     /gene="alaXM"
                     /locus_tag="VFJ72_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaXM"
                     /protein_id="HET7479875.1"
                     /translation="MTEELFLTDAYLQGFEARVVKLDGREVILDRTAFYPGGGGQPAD
                     KGTLGIGPVHAAVVDVRRESGDVVHVLDKAIPETVRDLKGGLDWERRYAHMRYHTALH
                     VLSGVIWRNFDAKVTGGQMRAERARMDFSFPGEWTADVVDGIERLTNEALSEERPVRA
                     YELPREEALKNPDLIRTQVNLVPERVKTVRIVEIEGIDTQADGGTHVANTSEVGEIEI
                     TTHKSKGRQNKRIEFTLR"
     gene            complement(10740..>11115)
                     /locus_tag="VFJ72_10235"
     CDS             complement(10740..>11115)
                     /locus_tag="VFJ72_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479876.1"
                     /translation="NVREAALSGRFEVLGHPDLIKIFRKFPDRDITPMLEETADAIAE
                     SGIVVDVNAAGLRKPIGEIYPSRKFLEMFHRRGVPIILSSDAHAADQVAAGYDTSLKL
                     VHDVGYREVVTFKDHERGTLSL"
BASE COUNT         1811 a         3419 c         3793 g         2092 t
ORIGIN      
        1 cgcgtcgcgg atcaagttag ccatcatcgg ctcggtgcgg cggtggtagg cgtagacttc
       61 ctccagcgcg cttctcaggc gctcctccgg gtcaccggtc ctggcccacg gttcgggatc
      121 cggcggagga ttggcgccca tgtagtgctc cgagcaggcg gagaacagcg tcttctcatc
      181 cgggaagtgc ctgtagacgg tgagccgctc cacacccgcg ttctcggcga tggcgcttat
      241 agtcgtccgg gccatgccaa cggtcttgtg caactcgacg gtcgcctcgg tgatccggcg
      301 ccgcgtctcc tcctgcttct ccgcccgccg cttttgctcg tatttacgcg ccatagacca
      361 atctcgatat ttttattgaa caattgtgtt tattcatttg acagcgacct cccgttagat
      421 tattgtacta ccgaaataaa taaacgctac cgtttaatga attggaggag cgtctggata
      481 gaggagatcg aggcgggagt aggaaaggct atataggtga gtcagaggta aaggagcggt
      541 ggatgatcgc cgagaggata agcgagctgg agttgatgga ggtctggtac gaggaggacc
      601 cgacgatgcg catccgggtc accttccctt tctttttggg gaacgggacc aggagcacgg
      661 cggtcgtcta cttcgagata gatccggggc acaggctcgg cacccacacc gacagcgcgg
      721 aggagataat ccttatcctg gaggggacgg tcgaggtctc tctgggtgat gaggcggggc
      781 gtctctcagc gggcgagatg gcgctggtgc cggcgatggc gccgcacgga atgcgcaacg
      841 cgggtgatga gacggtccgg gtcgtcgggt tcttctccag caacgtggtg gttagcacgt
      901 tcgaccggcc catgatgccc ttcggccagc gggccgtcgg tacgccgccc gtcctcgaag
      961 aggagcaggt cgctaccggg gacagcggag cctagaagga ggcgagaaat ggcgcgcata
     1021 cagaccgtgc ccgcggatga ggcgcgaggt ctacgccggt tcatgttccg tgggatccaa
     1081 caccagtacg gtggggttgt tcccggcatc ttcgagatct tgatgccgga tctacaggtg
     1141 gctttcgcct cgggctggct ctacaaccgc ctccacatgc gcaagtcctc gcccatgacc
     1201 cgcctgcagc gcgagatgct cgccacggtc gtcaacggcc tcgtcggcgg cgctccctga
     1261 ctgggtcttc atacggcggc cgtgcgtcgc ctgacgaaag acgaacacct ctcggcggag
     1321 ttcgcaacca cctggcccat gtacgacctc gacgagaaga ctcgggcgct gctcgcttac
     1381 gccaagaagc tcacggagga gcccggcatg atcggggacg aagacatcga agacttgcgg
     1441 gcggcaggat gggacgagcg ggggatctac gaggcgacgg ccctgatctc gttcttcaac
     1501 ttcacaggga ggatggaggc cgcttcgggc ttgccgccgg acgatatacc caagggggca
     1561 agcttcgccg aggcggtatc cgatggcaaa agataacttc tagttcttct ggttctcgac
     1621 ggtgatgtct caaagatcac tcatttgggg gatgtgccgg attatatgac ctgccatctt
     1681 taggagaaac acgcgacgga aggagtaagc agaatgaaac cctgggtgaa ggttctgatc
     1741 gtcaccgtca ttatcgcggc tccggccatg gtgctgggcc gcgtcatctg gcctcccgcc
     1801 gaaggtggtc ccgaaccctc cggcgctcag ctaccgttct tcctcttcct agccctcatg
     1861 gagtctattc ttctggggct cgggatctcc ttcctggcct tcggactgcc ggcggtgcgc
     1921 agggtctccg ccgactcggg actcagagcg tgggtcatgt acctcgctat cggctggttg
     1981 atggtctcct ggtggcctca cgacaacatg cacattcaca acggcgagaa catgcagggt
     2041 cttctctaca tcgaatacgg ctttcacctg acgttgatgc tcacagggat tatcctggcc
     2101 tactgtttcc tgtcgatctt gcgggaaggc tccggcaagc cagcagtcgt aagatagagg
     2161 ggcagccgca actcaacgag gaggagacag cccatcgcta gatcaggcga agtgatcgag
     2221 aacccggttc agggagatag gatcgtcttc cgggagacgt cccaggatac cggcggcgag
     2281 ttgttgcgca tggaggtggt cgccttcccc caggcatcgg ggccggtgga ccacatacat
     2341 ctgcggcaag aggagcgatt cgagatcctc tccggcaccc tgaacgcccg tctgaacaac
     2401 caggagcaga gcttcggtcc gggagagact ctaaccatcc ctccggggac gccgcattcc
     2461 tggtggaacg gcggcaccga ggaagcccgc gtgctctgcg aactcaggcc cgccctgaac
     2521 acggagacgt tcttcgagac catctacggg ctcgcccgcg acggcaagac caacaaagag
     2581 ggcgtaccga atctgttgca gatagcggcc atgtccaggc tgcacagaga cgagatctat
     2641 ctcgcccggc ctccgatagc agtgcagaga gtcctgttcg ccttactccg ccccgtagcc
     2701 cggctgctcg gctaccggtc cagctacccg gagtacagcg gcagcctgta ggatacatcc
     2761 atccgctccg gtagaggaga ggaacattgg agatcgaagc caagctcgaa cagctcgggc
     2821 tcgtgctgcc ggccccgatt caactgccgc cgggagtcga gttgccgttc gcgtgagtgc
     2881 gggtgcgcgg cgaccgggcc tacgtctccg gccacggccc gctcaacccg gacggttctc
     2941 tctcgggacc gttcggcaag gtgggcgcgg aggtctcgcc ggaggagggc tacgaggcgg
     3001 cgcgaggggc ggcgctctcc gtactcgcaa gcctaaagcg ggagcttgga gacctcgacc
     3061 gggtcagcgc gtggctggta gttcacgggc tcgtcaacgc cgcgccggac ttcgggggca
     3121 cggcaggggt aatcaacggg ttctccgacc tgatcctgga catctacggt cccgaggccg
     3181 ggatgcacgc ccgctccgcc cccggcgtcg cgacgctgcc gctcaaccag ccggtcttcg
     3241 tcgcggcaga ggtcgaggtc cgtccgtaga gaaagtatga ggccccgcca gatggcgggg
     3301 ccccttggta taaacctcct tttatcgtgt gcttactgct tgggagttgc tcctaaggca
     3361 acgatggcgt ccatatccac cctggtgccg gttgaagagg cattcttcgt accgagtatc
     3421 tggacggtga tcgtgtgctc cgcgggaccg ttgaggttgt tcgccgcgaa gacgaccttg
     3481 gccggctgcc gggtcgcgct gtacagatcg acctgctgcg caggcccgcc gtcgaccgtg
     3541 accgtggcct taccgcggtc ggggccgagc gtggagaccc aggcgatgga gcttccgggg
     3601 accgtgtagg tcagcttgtt gaactgaacc ttgtccttgg ccgtgctcgc gaagctgacg
     3661 gagccgccgt acgccccggc gagtgcctgc gtcgtccagg tgccgccgta gctgaggttg
     3721 gccctgttgc tgtcgtcgat gggcgccagc ttgaagttgg gtccgctaac ccaggcgctg
     3781 cagtcggcgc cgttgcaggc ccgcacctgg aactgatacg agctcggcgc tgcagtagtc
     3841 cccatcgcca ggttgagcgt agtggacgat gcggtaccgg gctgcggcgt tacgtcggtg
     3901 aatgctccgc cgttggtcga ctgctgaagc tggtaggacg ttaccgtgcc ggtcgatgcg
     3961 gtccagttga gcttcaccgg gatcttgctg ttcgcaagcg cagcattgtc aacgagaagc
     4021 tgcgacacgg tgggcagcga ctgggccggt gctcccggcg cgctcggtgc ggcggcgttg
     4081 atgccgtttc cggtaaggct tgtgctgata tcgccctggt tcgcggcgtt ggtccgcagg
     4141 ttgaggctcg ccgtcctcgc gccgaccgcc ttcggcgaga aggtcacgtt gacggtgcag
     4201 gaggcgccgg ccgcgagggt cgccgggcag gtcttcgagc cgatggcgaa gtcgcccggg
     4261 ttggcgccgg cgatgtacgt atccgacacc ttcacgtcgc tggtgcccga gttggagagc
     4321 gtgatgttct tgggggtgct cgtcgtggcc gggaccgcgg actggttgcc aaagttgagg
     4381 ctcgccggag tcgcggacgc ctggggcgcc gagggctcta gcggggccgt gcagtctggt
     4441 gtcgagggtc caccgacgac tccgggaccg ttctcgtaga tcgtcagctc gttgccgacg
     4501 agcggggcgt ggcccagcca gaccgcgcga gactcggccg cgagggcgcg ggtcacgtcg
     4561 gcggcggaga ggcccttgta ggtggccgtc cacttggtcg agccgggggc gtcgtaggtg
     4621 agcgttccct ctgcgcccgc accggcacgc agcgtacgct tgccgttgag gtcgaacagg
     4681 tccttgccgg agacgagccg ctgctcgagc tggccaatgt ccaccggtcc gcccgttgcc
     4741 gggtcgacgg cggtgccgtg gatgacgacc gtgtcggacg cggtctgtac cggcctctgg
     4801 gccgttacgt catacgacgt ggtctgatcg ggcagggcgc cgctgttggt cacggtcagg
     4861 cgcaccttgt caccggggcg gatgtccggg gtgttgacgt tccagcagcc gccaccgggg
     4921 tggttgacct cgacgagtcc gtcgaaaccg ggagtgccgg ggtcgtcctg cggggtgatg
     4981 tccggcgtgc tgctcactac gttgccgccc cggacgatgt ccacgcggac ctggtcagtc
     5041 tgcgcgtagc cggtggcgga gacgaagtcg cgctgcggga acgagatgat cgagtgcggc
     5101 gacttgggcg gctcgttgac cgcgccgggc gccggtggaa cgtcgacggt gttggccgtg
     5161 acactgttgg aagccgccga gtggttgtcg gccgcgtcga aagccttgac ggtgtagttg
     5221 taggtaccct gggccacgcc ggagtcggtg aacgtgagcg ggctgtcggc cagcctgtcg
     5281 ccccggacct cgccaacgag cttgtcgtca cggtaaacgc cgtagccgat gacgtcggcc
     5341 gaggaggact tcgtccaggt cagcttgacg tcgttgccgc cgagcacggt ggcgtccagg
     5401 ttggtgggcg cgtcgggcgc ggtggtgtcc gcagcgggag ccgggggctt aaaggccgtc
     5461 ggcgccgtgc agggggcgga tggcccctta tctaccggca cggctgtcgt gccgacctcg
     5521 tagatggtca gttctgcgcc ggccagcggg tcgtgcccga gccagaggac gcgggactcg
     5581 gcccggttca acgcgcggtc catgtccgcg gcgtcgatgc ccttataggt ggccgtccag
     5641 ttgatagagc cggggacgtc gtaggagacg tcgccgttac cgggcgcgcg cagggtacgc
     5701 ttgccgctga ggtcgaacgc gtcgcggttg gcgacgagcc gctcctcgac ctggccgagg
     5761 tctatcgggt tgcccttcgc atcctgggcc gtgccgtgga tagctatgct gtccgggccg
     5821 gtctgaaccg ggcgcttcgc cgtcacgttg gagacggtag tctgatcgcg gtcgccctta
     5881 ttggtgacca gacggatctg atcccccggc atcatccagg gcgtggtgcc ctcccagcag
     5941 ccgccgccgg ggtggttgac ctcgacgaga ccgtcgaagc cgtcggtgcg ctcgtcgtcc
     6001 tgtggtataa ggccggtcgc actgccgatg atggcgccat cgcggatgag gaagacatcg
     6061 acgatgtcgt ccggggcgta gccctgcgcg gagacgaagt cgcgctgcgg gaagccgatg
     6121 atcgagtgcg gcagaaccgg cggctcattg accgcggtga ttggcgcggg gttgaccgta
     6181 gtcgtcaccg ttacctcatt ggagaggggt ccgcggttgc cgaccgcgtc ggcggcgtca
     6241 acggtgtacg tatagtcgcc cgcgggaagg ttcttgtcca cgaaagtggt cggggcgggg
     6301 gccgtgccgt ccggcttctg cacgttggct atcgccacgc cgttgcggta gatgccgtag
     6361 ctcgtgaccc ctacgttgtc ggtggcggcg gtccagtcca gcgtaacact gctcaggttc
     6421 gccgtggccg acagcccgct cggagcggtc ggtggctcga gctcgctgcc cggaggcggc
     6481 gggagcttct ccagcggcgc ggtgcacggg gcggcggggc cgggtatggc gccggcgccg
     6541 ttttcgaaga tcgtcgactc aaccgcggca agcgggttag acccgagcca cattccgcgc
     6601 gactcggcgc cgagcgcctt ggtgacgtcg gcggcggaga ggccggtgta ggtggcagtc
     6661 cagttgatgg agccggaagc gtcgtaggag atgtcgccgt tgccgggcgc gcgcagcgtg
     6721 cgcttgccgt tcagggcgaa ctggttgttg gggacgacga gccgctgctc gagctggtcg
     6781 agcggcagcg ggttgccggc cgcgtcctgg gccgtgccgt gaacctggac ggtgtccggc
     6841 gccgtctgga ccgggcgctt cgcggtcacg tccgccactg tggtctcgtc ggcgacacca
     6901 ctgccttcga cggtgatgcg gaccttatcg ccggccctga tgtcgggggt aacgccgacc
     6961 cagcaggcgc cgccgggatg gttgacctcg actaggccgt cgaactccgg cgtctgggga
     7021 tcgtcctgcg gaacgatacc ggtcactctg ccaacctcgg tgccgtcctt gtgcacgacc
     7081 gagacggtca ccttatcggt tgccgcgtac ccgctcgcag agacgaagtc gcgctgcgga
     7141 aacgcgatga tcgagtgcgg cgcgacgggc ggattgttga tggtcggcgc cgtctgggct
     7201 aaggcccgcg gtatccatac cgtcagagtg acgagggtga cgagcaatgc caccagcagt
     7261 tttttcgtgg cgccccctcc cctgctcagg tatggtcggt aatcggttgt catttgcttg
     7321 atcctttgtt tgatgttttt tgatgttttc acttgtcctc cgtcgagcac ccgcgatcag
     7381 ggaccacgag ataaaggaga gcgtcgcatc aaggatgatg cggtcggggc acgctcgatg
     7441 aagcaaggat tgttggcgac ggtagttata ggcacggttc ctccatcctc tatccctggc
     7501 cggtaaaacg gcgttctgcc acgagcagaa tgatcggtgt agggacagcc cattacatcc
     7561 agataactaa agaacttttc gtagctaaag gaaggtaggg ttttctagcc catctggttt
     7621 aatcagtcct atttaaaata ggctagaaat cagagggggc cgtcagcaag tgaagcctgt
     7681 ctctccggcc ggatccgggg ttagccgact actcgaagag cgtcggatac aggttgtcga
     7741 agaagaggcg cctgacgagc tctccccggc tcctcactcc gaccttctcg aagacgtttc
     7801 ggaggtggtt ctgcacggtg tattcggaga tgtagagtgt ccgggagatc tgcgcggtcg
     7861 acagaccgcg tacgatgaga ccgacgacct cttcttcacg cgcgctgaga ccgtaggaga
     7921 tcatgctgaa gggcagcaac tcctcgggct tcgtgggctc tatgacgatt accgtctccg
     7981 cgcgccgctc cggggtcgct tcggtcaggg agccgtagag cgtgagccag cgtccggagc
     8041 gcgtccgggc ccgcaggcgc gggatgctct cggtatcccg ctcgcgctcc ggcctgagcg
     8101 cgcgccggag cacgaccatc gccatacgga gcgtgggagg aagccccgcc cactcccgcc
     8161 atccggggcc gaggtcttcc agttcccgca gccaccgctc ggccgccgcc gtgtgctgaa
     8221 ccacccgccc ggacccgtcg atagtgagga cccccggcac gtccgcgccg gtggggttga
     8281 cgggagcctg cgtccgaagc gcagccgttt tcaagccgct gcccaaatgc ggcgctatgc
     8341 ggcgaaggag cgcgacctga cgaggtccgt agtccggccg gcctttctcg cggatcaggt
     8401 ccatagaccc ccagaggtac ccgccggcgc cgaagacgcc gcgcatctcg tgggcgagtc
     8461 cgagcgggcc gaggagctcc aggtagcgca gcgagcgttc aagacggcca ccggcggtct
     8521 cggagagccg cgccaccgcg cggtggctct gcgccatctg cttgtacagc ttcagatcgt
     8581 gctcgaagta gatgcgctcg aagaagatgg cggcctcttt cgcgcctccc atctcatcgg
     8641 agatcggatg tgtcagcaag ccgctcgcgg ggtccatggt agaggcgcag tacgcctcga
     8701 acggcaccgc gcgccgaaga cgcttgatca cctcatccag caacactggc ccgtcgagtc
     8761 ccgcgtagca cagacgcttc acctcggaga aagtccgctc ctcgacgtcc actgccatcg
     8821 ctctcctcct aaatagcccg cacacgattg tgcctcgcgc tccggggtcc gcgcatcact
     8881 caaatggatg atcttttacc catccatgga tccatgacgg tcggagaaat gacagagatc
     8941 tgccataccc catccccggg tccggctcta cagttagaga tgcaggtgga cgaatccaga
     9001 ggaaggagag caccatggag actacggagc acgccgtcac ccactccccg gatggcgagg
     9061 gcaagacggt gtggatggtg gggacggacc tgatcacctt caaggccacc ggcgaagata
     9121 ccgaaggggc attcgccctc ttcgactcgc tggtgctgcc ggggggcggc ccgccgccgc
     9181 acatccacca ccgggagagc gagagcttct acgtgctaga aggtcggttc gagttcctgg
     9241 ccgagaacgg atggataaag gccgccccag gttcgttcgt ccacgtcccg aaaggaaccc
     9301 tgcacacgct caagaacgcc ggagagggcg tcggaaggct actcaccctg gccatgccgg
     9361 cggggctcga ccggttcttc gaggaggccg gggagcccgg aacggacgtc tccacgcctc
     9421 cggggccgcc ggacgtggag aagctgctgg cgaccgcgga gcgatacggc atcgagttcc
     9481 ctccacctcc cgcgcagtaa atcgccgggg taacagggcc aggcgaagcc tattcgggta
     9541 gctcggccct ggctcccacc tttaccccgt gctcctcgaa gtagcccttg ttggcttcga
     9601 gggcgtatct ggcgggcccg gccgagacgt agtgcggcgg ctggtcgtcc agggccttca
     9661 tctcctggag gtctatgatg cgtccatcgg agtcaatgta ggcgatggag agcgggatga
     9721 gggtgttctt catccagaag gagcgcacct gctcgtccgg gaagacgaag agcatacccc
     9781 ggtccttgcc gagggacttg cggaacatga ggcccttctc ctgctcggcc ggggtgtccg
     9841 ctatctcgac ccttatcttc accttctctc cacccgaggc gtctatggtt acggtgcgga
     9901 gcccggacgt ggggccggtg gtctcgcccg ccgaagcctt cgtagtatcg gcgccgcggc
     9961 tggagtcgcc gggacctccg ccgcatccgg cgagcagcaa caacgccgct atgacgacta
    10021 tcgtcagggt tctcatcgca gcgtgaactc gatgcgtttg ttctggcgtc ccttgctctt
    10081 gtgggtcgtg atctctatct cccctacctc gctggtgttg gcgacgtgcg tcccgccgtc
    10141 ggcctgggtg tctatgcctt ctatctcgac tatacggacc gtcttcacac gttccggtac
    10201 gaggttgacc tgcgtgcgga tgaggtcggg attcttcagc gcctcttcgc gcggcagctc
    10261 atacgccctg accggccgct cttcggacaa cgcctcgttg gtgagccgct ctatcccgtc
    10321 caccacgtcc gccgtccact cgccggggaa agagaagtcc atccgggcgc gttcggcgcg
    10381 catctggccg ccggttactt tggcgtcgaa gttccgccag atcacaccgg agagcacgtg
    10441 cagcgccgtg tgatagcgca tgtgcgcgta acgccgctcc cagtccagcc cgcccttgag
    10501 gtcgcgcacc gtctccggga tggccttatc gaggacgtgg acgacatctc cgctctcgcg
    10561 ccgcacgtca acgaccgccg cgtggaccgg cccgatgccg agcgtccctt tatccgcggg
    10621 ctggcccccg ccgccggggt agaaggcggt ccggtccagg atgacctcgc gcccgtcgag
    10681 cttgacgacg cgggcctcga acccttgcag gtaggcgtcc gtgaggaaaa gctcctcggt
    10741 cacagtgaca gggtgccgcg ttcgtggtcc ttgaaggtga cgacctcgcg gtagcctacg
    10801 tcgtggacga gcttcaggct cgtgtcgtag ccggcggcga cctggtcggc ggcgtgggcg
    10861 tcggaggaga ggatgatcgg gacgccccgg cggtggaaca tctccaggaa cttccgggat
    10921 gggtagatct cgccgatggg cttgcggaga cccgccgcgt tcacgtccac gactatgccg
    10981 gactccgcta tggcgtcggc ggtctcttcg agcatcgggg tgatgtcccg gtcggggaac
    11041 ttgcggaaga tcttgatcag gtccgggtgg ccgaggacct cgaagcgccc ggagagcgcg
    11101 gcctcgcgga cgttc
//
LOCUS       DASSMB010000172         9661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_547763, whole genome shotgun sequence.
ACCESSION   DASSMB010000172 DASSMB010000000
VERSION     DASSMB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9661
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547763"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3185)
                     /locus_tag="VFJ72_10240"
     CDS             complement(<1..3185)
                     /locus_tag="VFJ72_10240"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7479877.1"
                     /translation="MLLESLNPTQLDAVKHTEGPLLILAGAGSGKTRVLTHRIAYLLE
                     QGLAGPEEILAITFTNKAAREMKERVALLVGPDSRKMWVSTFHAFCARILRAHADKLG
                     YRREFTIYDTADQVRLVKRCIIELGKDPKRFNPRSFQAQISDAKNRLLAADDFLRQTE
                     GYMAENVAEVYDLYQQRLYENNAMDFDDLIMQTVALLEIFPEVRERYQTRFRYIHVDE
                     YQDTNHAQYRLVNILAAARRNLCVVGDDDQCLVEGTPVMMADGSEKPIEDVAEDDEVL
                     SAYGSGDFRPARVTAVARRERVDGVRISTRLGREVVSTPEHTHFAGYRLGLTPQMHFT
                     YLMHKSDVGFRLGTSQVYTNGRVKPTVGFAQRCRQEHGDAVWVLSSHASEIEARAEEM
                     VLSLRHRIPTLPFVPRKGRSSGGLVSDEAHIKRVFSLFDTGSSAMDLLAERGLHPEYP
                     HYRPQSYTGRRRNLVVTLCGDRRGASPMHRISMVGRDEEGRSALESLGLSVRPAKQGS
                     VSWRFETCYKDFGRLMQVAERIREVLDVSLVFQARLGGSRGWREANSLPFVPAGSVRP
                     GMVMFAGDGEYDVVESVERILLDRPVYDIDVEGTHNFVAGGIVTHNSVYSWRGADIRN
                     ILDFERDYPEATVVKLEQNYRSTQTILSAANAVVANNASRKRKELWTAGDEGERIRVF
                     TASDEYAEARFVVAEVERLMEGGVTARDIAAFYRTNAQSRALEDVLVREGVPYQIVGG
                     VKFYERAEIKDAMAYLSVISNPNDSVSLERIINVPKRGLGNTSVAKLQDYASKNGISL
                     YEALGEAGEVDITAAARKSCRALYELFEGWRVAAKEVPPAELIGAVLDESGYKKELDS
                     EGTVEAESRLENLEELINAAREYESVEPDATLDGFLQEQALYSETDKVGVEGRVTLMT
                     LHNAKGLEYDHVFVVGMEEGTFPHARSLDEQNLEEERRLCYVGITRARKTLTLSYAKL
                     RSNWGEREYQMPSRFLSEIPDEYKSGTISGGTTAAAGRGGWGRALPKRGSSSPEPASD
                     SPSFRAGEKVRHAKFGEGEILESGAGKVVVR"
     gene            complement(3215..3988)
                     /locus_tag="VFJ72_10245"
     CDS             complement(3215..3988)
                     /locus_tag="VFJ72_10245"
                     /inference="COORDINATES: protein motif:HMM:NF021788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7479878.1"
                     /translation="MAATASPSSQRKNASAASRTQRAPHIPRQPLPSTKIISLDHLRL
                     HLEDRTHLTTSTVEVGGGNYRITHPAAADALIDEAEFDHDERLPYWADLWPSAIALAR
                     SIADQNLAGRRVIELGCGLGLPSVAALARGAEVLATDHYAATLDFAAHNARTNVGRDL
                     RTALLDWRSPEIESLGVFDLVIGADVLYEALSGLALAELVPRLLSPRGEAVFADPNRN
                     TAPVFLDEMEEQGFRTTTEIVSVEQGGRGVEIGLHRLRN"
     gene            complement(3885..4355)
                     /locus_tag="VFJ72_10250"
     CDS             complement(3885..4355)
                     /locus_tag="VFJ72_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET7479879.1"
                     /translation="MRLTKKSKYAVRALMELALAECGSPRGVADIARCQKIPERFLEQ
                     IFGELRRAKILESHRGAHGGYCFAVPTEEITVLDIVEILDGEVRPARCSAGGVCYIAE
                     APLCATSKVWDEARVALESVFGRYSIAQLAEEERERRVAHAAGAAYPETAASLN"
     gene            complement(4455..5153)
                     /locus_tag="VFJ72_10255"
     CDS             complement(4455..5153)
                     /locus_tag="VFJ72_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HET7479880.1"
                     /translation="MPRESFQHELDELVSQVLSLGGEVENSLVNMVTAMEERDADAAS
                     RELGVDVRYKMRGAEIDEECMILQARQAPVARDLRLLYTVQAVTNHLVRSGTLCEHIC
                     HGIADTADHERDEDLEAALIEMARSARNLFHEGLDIFKDRDIDRARDLQAVDDKVDLL
                     YSEALNLIANPSETGAGSPEWRMRAALMVHYLERIADHGVDIGGRTVFLVTGERIENA
                     MQQYMKREQRPEEE"
     gene            complement(5157..6041)
                     /gene="pstB"
                     /locus_tag="VFJ72_10260"
     CDS             complement(5157..6041)
                     /gene="pstB"
                     /locus_tag="VFJ72_10260"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET7479881.1"
                     /translation="MDSRDKTREQQMTQRPTNEPEPRREEAPRRSSGIHDTEPVVTVE
                     NLDMYYGSFKALQNVSMNIKRNRITALIGPSGCGKSTFIRSINRMHEIVPGARVEGKI
                     LLDGEDIYGDDVDPVRVRRKVGMVFQKPNPFPTMTIYDNVIAGIKLGRKRKKDELDGI
                     VEKSLRQAALWDEVKNKLKDSGTSLSGGQQQRLCIARTLAIEPEVILMDEPASALDPI
                     STQKIEDAMDELKELYTVVIVTHNMQQAARISDFTGFFYIENMGDPGRLWEFDETEKM
                     FSNPDRSETEDYVTGRFG"
     gene            complement(6081..6950)
                     /gene="pstA"
                     /locus_tag="VFJ72_10265"
     CDS             complement(6081..6950)
                     /gene="pstA"
                     /locus_tag="VFJ72_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7479882.1"
                     /translation="MSSLSTTSSRYRQRKMVDRIMTGIAYACTAVAILPLTWIIIYVV
                     IKGLGAWDLNFFTALPQSFGDGGGVRNGIVGTLVIVGLATLIGVPVGVMAGIYLAEYG
                     NNRLGYVIRFMADTMTGIPSIVVGLFAFGLVVGITGGFSALAGAFALAVLMIPVITRT
                     TEEIIRLVPGSIREASLGLGVSRWKTIMRVVVPTALSGIVTGVLLAVARVAGETAPLI
                     LTIFGTNFPLSSNLVNAPYTTLSLQIYQLGKAPSPDVVEVAWGAAMLLVIIVVGTSIL
                     ARLIFKGQNVSGR"
     gene            complement(6947..7891)
                     /gene="pstC"
                     /locus_tag="VFJ72_10270"
     CDS             complement(6947..7891)
                     /gene="pstC"
                     /locus_tag="VFJ72_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7479883.1"
                     /translation="MSFFKELRRGHRSDTIFAGVALLFGLSILALMIGIIAELWQESL
                     AARQEFGLSFLWSTEYNPGQDLYGALPFIYGTLVTAFISILIAGPIGVGIAAFLVEIA
                     PDRVNRMVGFIVELLAAVPSIVYGLWGFFALAPFMRDYVVPPLQSVLGWTPLFKGTFP
                     GPTVFTASIVLSIMILPTVAAVSRDVIRAVPRDQREGMLALGATRWEMFRGAVLPYAA
                     SGVLGAVILGLGRAAGETMAVTFIIGNKPHIFSSLFDQGATLASQIAGQFPEAGGISL
                     SFLVELGLVLFVITIIINITARLIVSLVSKTPKGGIRA"
     gene            complement(8030..9148)
                     /gene="pstS"
                     /locus_tag="VFJ72_10275"
     CDS             complement(8030..9148)
                     /gene="pstS"
                     /locus_tag="VFJ72_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET7479884.1"
                     /translation="MKGVLRSRLLVMMGLLALSVVLAACGQATGGGGGGSDSGDAKAA
                     SQISGAGSSFVAPFMDKMGQELVKDGGPQVNYQSIGSSGGREQFIQKTVAFGASDAPM
                     DSEEEKQAGGNPKHIATVGGAVVAAYNLEDLGDTPLNLTGTVLADIFLGKITNWNDPA
                     IAELNPNATLPDAQISVVHRSDGSGTTNIFTNYLAAVSPAWKNGPGPGDELQWPTGIG
                     ADGNEGVAGQVQQTKNSIGYIGLEYAKENDIPFANIGEKTGSYVQPSVKSAQTAIEQA
                     GENGDIPDDLKVTISSLAPFEGDAYPITGLTWMIVRQQMDDLAECEAVAETAWYITHD
                     GQDFAEDLNYVELPDDIVSADEKTIKGMEAGGKKCYTK"
BASE COUNT         1703 a         3209 c         2930 g         1819 t
ORIGIN      
        1 aaacgaacga cgaccttccc ggccccggac tccaggatct caccctcccc gaacttggcg
       61 tgccgcacct tctccccggc cctgaacgag ggcgaatccg acgcaggctc cgggctgctg
      121 ctcccccgtt tcggcagcgc gcggccccat ccgccacgtc cggcagcagc agtggtcccg
      181 ccggagatgg tgccggactt gtactcgtcc gggatctccg acaggaagcg cgagggcatc
      241 tggtactcgc gctcgcccca gttggaccgg agcttggcgt aggagagcgt gagcgtcttc
      301 ctggcccgcg taatgccgac gtagcacagc cgccgctcct cttccaggtt ctgctcatcc
      361 agggagcggg cgtgcgggaa ggtcccctcc tccatcccca cgacgaagac gtgatcgtac
      421 tcaagcccct tcgcgttgtg gagcgtcatc agggtgacac ggccctcaac cccgacctta
      481 tccgtctccg agtagagcgc ctgctcctgc aagaacccat cgagggtagc atccggctcc
      541 acgctctcgt actcgcgagc cgcgttgatc aactcctcca ggttctccag acgagactcg
      601 gcctccaccg tcccctcgga gtccaactcc ttcttgtacc ccgactcgtc gagcaccgcc
      661 cctataagct ccgccggcgg cacctccttc gccgccacgc gccatccctc gaacaactcg
      721 tagagcgccc ggcaggactt gcgcgccgcc gccgttatgt caacctcccc ggcctcaccc
      781 agagcctcgt acagggagat gccgttcttg ctggcgtagt cttgcagctt cgccacggag
      841 gtattaccga ggccgcgttt cggaacgttg atgatgcgtt ccagggagac cgagtcgttc
      901 gggttcgaaa tcacggagag ataggccatg gcgtccttga tctccgcccg ctcgtagaac
      961 tttacgccgc ccacgatctg gtacggtacc ccctctcgaa ccagcacgtc ctctaaggcc
     1021 cggctctgag cgttcgtccg gtagaacgcc gcgatgtccc gcgctgtaac accgccctcc
     1081 atcagccgct cgacctccgc caccacgaac cgcgcctcgg cgtactcgtc cgacgccgtg
     1141 aatacccgga tgcgctcccc ctcgtccccc gccgtccaca gctccttccg cttgcgcgag
     1201 gcgttgttcg ccaccaccgc gttcgccgca gagaggatgg tctgcgtcga gcggtagttc
     1261 tgctccagct tgaccaccgt tgcctccggg tagtcccgct cgaagtccag gatgttccga
     1321 atatccgccc cacgccagct atagaccgag ttgtgcgtga ctatgccacc ggcgacgaaa
     1381 ttgtgggtcc cctccacgtc gatgtcgtag accggacggt ccagcaagat ccgctccacg
     1441 gactccacaa cgtcgtactc gccgtcaccc gcgaacatga ccatacccgg cctcacggaa
     1501 ccggcgggga cgaacggtag tgagtttgcc tcccgccagc cccggctccc gcccagccgc
     1561 gcctggaaca cgaggctcac gtccaacacc tcgcggatgc gctcggcgac ctgcatcaaa
     1621 cgcccgaagt ccttgtaaca ggtctcgaag cgccagctaa cggagccctg cttcgcgggc
     1681 cgcacggaga gccctagaga ctccaaggcg gaccgcccct cctcgtcgcg tcccaccatc
     1741 gagatgcggt gcatcggcga cgctccgcga cggtcgccgc agagcgtaac caccaggttc
     1801 cttcgccttc cggtgtagct ctgaggacga tagtgtggat actccgggtg gagacctctc
     1861 tccgccagga gatccatcgc gctgctcccg gtgtcgaaga gggagaagac ccgctttatg
     1921 tgcgcttcat cggagacaag accgcccgag cttcgcccct tgcgcggcac aaacggcaac
     1981 gtggggatgc ggtggcgcag ggacagcacc atctcctccg cgcgggcctc gatctcggaa
     2041 gcgtggctgg agaggaccca gaccgcgtcg ccgtgctcct gacggcagcg ctgcgcgaac
     2101 ccgaccgtcg gtttcacccg gccattcgtg tacacctggg aggtcccgag acggaacccc
     2161 acgtcgctct tgtgcatgag gtaggtaaag tgcatctgcg gcgtcaggcc aagccgatat
     2221 ccggcgaagt gtgtgtgctc gggggtgctg accacctcgc gtcccaagcg ggtggagata
     2281 cggaccccat ccacgcgctc cctccgggca accgccgtta cccgcgccgg ccggaagtct
     2341 ccactaccgt aagcgctcag aacctcgtca tcctcggcta cgtcctcgat gggcttctcc
     2401 gagccgtcgg ccatcattac cggcgtccct tcgaccaggc attgatcgtc gtcgccgaca
     2461 acgcacaaat tgcgacgcgc ggcggccagg atgttgacga ggcgatactg ggcgtggttg
     2521 gtgtcctggt actcatccac gtggatgtag cggaagcgcg tctggtaacg ctcgcggacc
     2581 tcggggaaga tctccaagag ggcgacggtc tgcatgatga ggtcgtcgaa gtccatggcg
     2641 ttgttctcgt agagccgctg ctgatagagg tcgtagacct cggcgacgtt ctcggccatg
     2701 tagccctcgg tctgccgaag gaagtcgtcg gcggccagga ggcggttctt ggcgtcggag
     2761 atctgggcct ggaacgagcg cgggttgaag cgctttgggt ccttgccgag ctcgatgatg
     2821 cagcgcttga cgagccgtac ctggtcggcg gtgtcgtaga tcgtgaactc ccgccggtag
     2881 ccgagcttgt cggcgtgcgc cctgagtatc ctcgcgcaga aggcgtggaa ggtggagacc
     2941 cacatcttgc gggagtcggg tccgacgagg agcgcgaccc gctccttcat ctcgcgggcg
     3001 gccttgttgg tgaaggttat cgccaggatc tcctccggtc cggcgagtcc ctgctccagc
     3061 aagtaggcga tcctgtgcgt cagcacgcgg gtctttccgc tccccgcccc ggccaggatg
     3121 aggagcggtc cctcggtgtg ttttacggcg tctagctgcg tgggatttag cgattccaga
     3181 agcattgttc aaaagtataa cagcagcgta ccgcctaatt acgcagccgg tgcaacccta
     3241 tctcgacgcc tctgccgccc tgctccacag agacaatctc cgtcgtcgtt cggaaacctt
     3301 gttcttccat ctcatccaga aagacgggcg cggtgttgcg gttcggatcg gcgaaaactg
     3361 cctctccgcg gggagatagc agccggggaa ccagttcggc gagcgccagt ccgctcaggg
     3421 cctcgtacag cacgtccgct ccgatgacga ggtcgaagac ccccaggctc tctatctccg
     3481 gcgagcgcca gtcgagaagt gcggtccgaa gatcccggcc cacgttggtc cgggcgttat
     3541 gggcagcgaa gtcgagggtc gctgcgtaat gatctgtagc gagtacctcg gctcctcggg
     3601 cgagcgcggc tacggaaggt agtcccaggc cgcacccgag ctcgatgacg cgacggccag
     3661 caaggttctg gtccgctatg gatctcgcaa gagcgatggc gctcggccac aggtcggccc
     3721 agtagggcag gcgctcgtcg tggtcgaact ccgcctcgtc tatgagagcg tccgctgcgg
     3781 cgggatgtgt gatgcggtaa ttgccgccgc cgacctcgac cgttgatgta gtcaggtgcg
     3841 tgcggtcttc gaggtgcagc cggagatggt ccagagagat gattttagtt gagggaagcg
     3901 gctgtctcgg gatatgcggc gcccgctgcg tgcgcgacgc ggcgctcgcg ttcttcctct
     3961 gcgagctggg cgatgctgta gcggccaaac acgctttcga gggcgacacg ggcttcgtcc
     4021 cagaccttgc tcgtggcgca cagaggagct tcagcgatat agcagacgcc gccggcggag
     4081 catcgggcgg gccggacctc gccgtccagg atctccacta tgtccaggac cgtgatctcc
     4141 tcggtcggca ccgcgaagca gtagccgccg tgagcccccc ggtggctctc caggatcttt
     4201 gcccggcgga gctcgccgaa gatctgctca aggaaccgct ccgggatctt ctggcagcgg
     4261 gcgatatccg cgacgccgcg cggcgaaccg cactcggcca gcgccaactc catcaacgcc
     4321 cgaacggcgt acttgctctt tttagtaagt ctcactacga aaaaccgcct gcttgcatac
     4381 cgaaccgtag ccccgattgt agcagatatg cacaggtaag gactgacgga caaaggaagc
     4441 gagggtagtt tacgctactc ttcctcggga cgctgctcgc gcttcatgta ctgctgcatg
     4501 gcgttctcta tgcgctcccc ggtcaccagg aagaccgtcc gcccgccgat atccacgccg
     4561 tggtcggcga tgcgctccag gtaatggacc atgagagccg cgcgcatccg ccactccggg
     4621 ctcccggccc cggtttcgga ggggttggcg ataaggttca gcgcctccga gtacaggagg
     4681 tccaccttgt catccacggc ctgcaggtca cgagctcggt ctatgtcgcg gtccttgaag
     4741 atatcgagac cttcgtggaa gaggttgcgc gccgagcggg ccatctcgat cagcgccgct
     4801 tcgaggtctt cgtcccgctc gtggtcagcg gtgtcggcga tgccgtggca gatgtgctcg
     4861 cagagcgtcc ccgaccggac caggtggttc gtcaccgcct gcaccgtgta gaggaggcgc
     4921 aggtcgcgcg ccaccggcgc ctgtcgagcc tggaggatca tgcactcctc gtctatctcc
     4981 gcgccgcgca tcttgtagcg gacgtctacg ccgagctcgc ggctcgccgc gtcggcgtcg
     5041 cgctcctcca tggcggtcac catgttcacc agggagttct cgacctcccc gccgaggctc
     5101 aagacctgcg agaccaactc gtccagctcg tgctgaaagc tctccctcgg catggtctag
     5161 ccgaaccggc ccgtcacgta gtcttcagtc tcggagcggt ccggattgga gaacatcttc
     5221 tccgtctcat cgaactccca gagcctgccg gggtcgccca tgttctcgat atagaagaag
     5281 cccgtgaagt ccgagatgcg ggcggcctgc tgcatgttgt gcgtcacgat cacgaccgtg
     5341 taaagctcct tcagctcgtc catcgcgtcc tcgatcttct gggtcgagat cgggtcgagc
     5401 gcgctggccg gctcgtccat gaggattacc tcgggctcga tggctagggt ccgggcgatg
     5461 cacagcctct gctgctggcc gccggagagg gacgtcccgc tgtccttcag cttgttcttc
     5521 acctcgtccc acagggccgc ctgccggagc gacttctcca caatcccatc caactcgtcc
     5581 ttcttgcgct tgcgcccgag cttgatgccg gcgatcacgt tatcgtagat ggtcatggtc
     5641 gggaacgggt tcggcttctg gaagaccatg ccgaccttgc gccgcaccct caccgggtcc
     5701 acgtcgtccc catagatgtc ttctccgtcc agaaggatct tcccctccac cctggctccc
     5761 ggcacgatct cgtgcatccg gttgatgctg cggataaagg tgctcttgcc gcacccggaa
     5821 ggtcctatga gggcggtgat cctgttccgc tttatgttca tggagacgtt ctggagcgcc
     5881 ttgaacgaac cgtagtacat gtccaggttc tcgaccgtca ccaccggctc cgtgtcgtgg
     5941 atgccggagg agcgccgcgg ggcctcctcc cgtctgggct ccggttcgtt ggtcgggcgt
     6001 tgggtcatct gctgttccct cgtcttgtct ctggaatcca tcggattacc acggtccttt
     6061 cgcgcgggta caccttacca tcacctgccg ctgacgttct gccccttgaa gatcaaacgc
     6121 gccagtatgc tcgtcccgac aactatgatc accagcagca tcgccgcgcc ccacgccacc
     6181 tccacgacgt ccggcgaggg agccttgccg agctggtaga tctgcaatga cagcgtcgta
     6241 tacggcgcgt tgaccaggtt cgagctcaaa gggaagttgg ttccgaatat ggtcaggatc
     6301 aggggtgccg tctctccggc gacccgcgcc acggcgagca acaccccggt cacgatgccg
     6361 cttagggccg tcggcacgac aacccgcatg atggtcttcc acctcgacac accgaggccc
     6421 agcgaagcct cccggataga gcccggaacg agccgtatta tctcctccgt ggtgcgggtg
     6481 atcacaggga tcatcagcac cgcgagggcg aacgccccgg ccagcgcgga gaagccgccg
     6541 gtgatcccca cgactagccc gaacgcgaac agaccgacga cgatggaagg tatcccggtc
     6601 atggtatcgg ccatgaagcg gatcacgtac cccagccggt tgttcccata ctcggccagg
     6661 tagatgcccg ccatcacgcc caccggcacc ccgatgaggg tggcgagacc gacgatcacg
     6721 agcgtcccga cgatcccgtt cctgacgccg ccgccgtctc cgaaggactg cgggagcgcc
     6781 gtgaagaagt tcaggtccca ggcgccgagc ccttttatga cgacgtagat gattatccag
     6841 gtcaaaggaa ggatggcgac ggcggtacac gcataggcga tgccggtcat tatccggtct
     6901 accatcttcc gctgccggta acggctcgat gtcgtggaga ggctgctcat gcccgtatac
     6961 ctcccttggg agtcttgcta acgagagaga ctatgagacg cgcggtgatg ttgataatta
     7021 tcgtaatgac gaagagcacc agcccgagct cgaccaggaa cgagagcgag atcccgccag
     7081 cctccgggaa ctggccggcg atctggctag ccagcgtcgc cccctggtcg aagagcgagc
     7141 tgaagatgtg cggcttgttc ccgatgatga acgtcacggc cattgtctca cccgccgccc
     7201 ggcccagccc caggatgacc gccccgagca ccccgctcgc cgcgtagggc agcaccgcgc
     7261 cgcggaacat ctcccagcgc gtcgcgccca atgccaacat cccctcccgc tggtctctgg
     7321 ggacagcgcg tataacgtcg cgggagacgg cggccacggt aggcaggatc atgatggaga
     7381 gcacgatgct cgccgtgaag accgtcggcc ccggaaaggt acccttgaac agcggcgtcc
     7441 accccaacac gctctgcaac ggcggcacga cgtagtccct catgaaaggc gccagggcga
     7501 agaagcccca gagtccatag acgatagacg gaaccgcggc cagcagctcg actataaacc
     7561 cgaccatccg gttcacgcgg tccggcgcga tctcgaccag gaacgccgcg atgccgacgc
     7621 cgatcggacc ggcgatcagg atagagataa acgcggtgac cagagtcccg tatatgaacg
     7681 gcagagcccc atagagatcc tgacctgggt tgtactccgt gctccacagg aagctcaggc
     7741 cgaactcctg ccgcgcggcg aggctctcct gccacagctc ggcgatgata ccgatcatca
     7801 acgccaggat ggagagcccg aacaacaggg ccaccccggc gaaaatggtg tcgctcctgt
     7861 gtccgcggcg cagttcttta aagaagctca aatcgccctc tctccggtgc ggggatccaa
     7921 aacccgaaaa ctaacgctaa atctcgtctt tgacaaggtc tacttccaga cgtggtccgg
     7981 gagagaaccc cgaaaggctc tctcccataa gccacgctag cctaatctgc tacttcgtgt
     8041 agcacttctt gccgccggcc tccataccct tgatggtctt ctcgtcagcg gagactatat
     8101 cgtccgggag ttccacgtag ttcaggtctt cggcgaagtc ctggccgtcg tgcgtgatgt
     8161 accaggccgt ctcggcgacc gcctcgcact cggccagatc gtccatctgc tggcgcacga
     8221 tcatccaggt gaggccggtg atcgggtagg cgtccccctc gaacggggcc agcgacgaga
     8281 tcgtgacctt caggtcgtcc gggatgtcgc cgttctcgcc ggcctgctcg atggcggtct
     8341 gcgcgctctt cacgctcggc tgaacgtagc tgccggtctt ctcgccgatg tttgcgaagg
     8401 gtatgtcgtt ctccttggca tactccaggc cgatatagcc gatggagttc ttggtctgct
     8461 gcacctgacc cgcgacaccc tcgttgccgt cggcgccgat gccggtgggc cactgaagct
     8521 cgtcaccggg tccgggaccg ttcttccagg ccgggctaac cgccgcgagg tagttggtga
     8581 agatgttcgt ggtgcccgag ccgtcagagc ggtgcacgac ggatatctgc gcgtccggga
     8641 gcgtcgcgtt cgggtttagc tcggctatcg ccgggtcgtt ccagttcgta atcttgccga
     8701 ggaagatgtc ggcgaggacc gtgcccgtca ggttgagcgg ggtgtcgccc agatcctcca
     8761 ggttgtacgc ggcgaccacc gcgccgccta cggtggcgat gtgcttgggg ttcccaccgg
     8821 cctgcttctc ctcctcgctg tccatcggcg cgtcggaggc gccgaaggca accgtcttct
     8881 ggatgaactg ctcgcgccca ccgctggagc ctatggactg gtagttgacc tgcggcccgc
     8941 cgtccttgac aagctcctgt cccatcttgt ccatgaacgg agcgacaaag gaggaacccg
     9001 cgccgctgat ctgggaagcc gccttggcgt caccgctgtc gctacccccg ccaccgccgc
     9061 ccgtagcctg cccgcaagcc gcgagcacca cggacagagc caaaagaccc atcatgacca
     9121 acagcctcga acgcaatacg cccttcaagt ccacgctcct ctcaaaaagg tccgaaccgg
     9181 ggactctccg tcccacttca gggcaggata atagggcatg gcaatctatc tttagtttca
     9241 gttatgtaaa ctgcaattta aatctatgtt aaatggagcc ctcgccggag cgggctggtt
     9301 gcagtggcac ttgcggcttc ttgcggagag ggcacacagt gcctttaact tggtgttgcg
     9361 gtgtatcaga cggcagtgag agcgaggagt agagtaacgg ctggccgccg tttgtattta
     9421 atagccggac ggacagcttt atggaggggg tcttggcgag agtgaacgta ccgggtacat
     9481 cctgaagagc agatctgacc gcgagttgat cctggcctgt cgcaagggcc ggagcggggc
     9541 gtggaaggag cttctcggca agtacgagcg gctggtctac tccatcccgc tgcgctacgg
     9601 gctctcgcgc cacgacgcgg cggacatcgc gcagctcacg tttaccatcc tgatccagaa
     9661 c
//
LOCUS       DASSMB010000173         2220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_674968, whole genome shotgun sequence.
ACCESSION   DASSMB010000173 DASSMB010000000
VERSION     DASSMB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674968"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..660
                     /locus_tag="VFJ72_10280"
     CDS             268..660
                     /locus_tag="VFJ72_10280"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7479885.1"
                     /translation="MAEPVERAGERTTPKDVFLSEVKTRDIFPLLILHLVREKPEYGN
                     SIMAQIREISAGAMSVSPNTIYPLLRRLEEKGYVKGEWERPDTRSRRFYTITPRGEEK
                     YQEIKDRFEVHLLRVRDALESMHGELYG"
     gene            680..>2220
                     /locus_tag="VFJ72_10285"
     CDS             680..>2220
                     /locus_tag="VFJ72_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET7479886.1"
                     /translation="MSHGIRKVAVLGAGTMGAAIAAHCANAGLEVDLLDIAPNEGDDK
                     DAVVKGGFDRMRKARPAALMSKAVAERMRIGNFDEDFDRVADADWVVEAILEKLEPKR
                     RLMERVEATAKKSAIISSNTSGIPLHQVAEGRSEDFKKRFLGTHFFNPPRYLKLMEII
                     PTEDTNPEVVEAARNFGERVLGKGGVIAKDTPNFIGNRLGSFAGMQSVTYAFENGYGI
                     EEIDAITGPLIGHPKTATFRLNDQVGLDIAVGVAENLYEAVPEDDAREDLKPPPKLKE
                     MQEKNLLGNKTGSGFYKRDKRDGKTVFDVLDLDTFEYRPAENPEIPIAKEAQQQGDLG
                     ARLRFLIEKADEDRHARYIRDTLLPYMAYASRRVPEISDTLEDVDHAMEWGFAHQTGP
                     FRTWDLLGVRDTVEEMNSLGIEVGGWVKEMLEAGNESFYKTEDGTELQFSPVSKQYEP
                     VREDPMYVSLDRLRDEGKELARNDSASLLDLGDGVLCLEFHSKGNSIDGAMVEMGNRA
                     LAELER"
BASE COUNT          471 a          646 c          761 g          342 t
ORIGIN      
        1 cgggatgacc tcccgatccg cgaacgcccg caccttgtcc cggacctcgc gctcctcgtc
       61 cgtgaacagg tcgtccagca agtaatagtc ggtccccaga gaccgggtaa cgtcggcgat
      121 catggactcc tccttctcat ggcctctccg cgccccaact ctagcacgct acccgtgcca
      181 ccggcgcccg gaaactttac gtgaatttat tatccatgtt attatcaata ttgacaagtt
      241 tttcggaaat cgaaggaaga ggagtcgttg gcggagccgg tagagcgggc gggggagagg
      301 accacgccga aggacgtgtt cctgagcgag gtcaagacgc gggacatctt cccgttgctc
      361 atactgcatc tcgtgaggga gaagccggag tacggcaaca gcatcatggc gcagatacgc
      421 gagatcagcg ccggggcgat gagcgtgtcg ccgaacacta tctatccgct gctccggcgg
      481 ctggaggaga agggttacgt gaagggagag tgggagcgcc cggacacgcg gagccgccgt
      541 ttctacacta ttacgccgcg cggagaagag aagtatcagg agatcaagga cagattcgag
      601 gtgcatcttt tgcgggtaag agatgcgctg gaatccatgc acggagaact ctatggttaa
      661 cacgggagaa ggaggccgga tgtcgcacgg gatcaggaag gtagcagtcc tcggagcggg
      721 cactatgggg gcggcgatag cggcgcactg cgcgaacgcg ggtctggaag tggacctgct
      781 cgacatcgcg ccgaacgagg gcgacgataa agacgccgtc gtcaagggcg gcttcgaccg
      841 gatgcggaag gcccgtccgg cggcgctgat gagcaaggcc gtggccgagc gcatgaggat
      901 cgggaacttc gacgaggact tcgaccgcgt cgcggacgcc gactgggtcg tagaggcgat
      961 cctggagaag ctggagccga agcgccggct catggagcgg gtcgaagcga cggcgaagaa
     1021 gagcgccatc atctcctcga atacctcagg gattccgctg caccaggtcg cggaggggcg
     1081 ctcggaagat ttcaagaagc gtttcctggg gacgcacttc ttcaacccgc cgcgctactt
     1141 gaagctgatg gagatcatcc cgacagaaga cacaaatccc gaggtagtcg aggccgcgcg
     1201 gaacttcggg gagcgggtgc tcggcaaggg cggcgtaatc gccaaggaca cgccgaactt
     1261 catcggcaac cggctcggga gcttcgcggg catgcagtcg gtgacgtacg cgttcgagaa
     1321 cggctatggc atcgaggaaa tagacgcgat caccggcccg ctcatcggcc atcccaagac
     1381 ggcgacgttc cggctgaacg accaggtcgg cctggacatc gccgtcggcg tcgcggagaa
     1441 cctgtacgag gccgtcccgg aggacgacgc gcgcgaggac ttgaagccgc cgccgaagct
     1501 caaggagatg caggagaaga acctgctcgg caacaagacc ggctccggct tctacaagcg
     1561 cgacaagcgc gacggcaaga ccgtcttcga cgtgctggac ctcgatacct tcgagtaccg
     1621 tccggcggag aacccggaga tcccgatagc aaaagaagcg cagcagcagg gcgacctcgg
     1681 ggcgcggctg cggttcctca tcgagaaggc cgacgaggac cggcacgccc gctacatccg
     1741 ggacacgctc ctgccgtaca tggcctacgc ctcgcggcgc gtaccggaga tctccgatac
     1801 gttggaggat gtggaccacg cgatggagtg gggcttcgcc caccagaccg gccccttccg
     1861 cacctgggac ctgctcggcg tgcgcgatac cgtcgaggag atgaactctc tcggcatcga
     1921 ggtcggcggc tgggtgaagg agatgttgga ggccggcaac gagagcttct acaagaccga
     1981 ggacggcacg gagctacagt tcagtccggt gagcaagcag tacgagccgg tgcgcgagga
     2041 cccgatgtat gtctcgctcg accggctgcg cgacgagggc aaggagctgg cgcgcaacga
     2101 ctcggcgagc ctgctggacc tcggcgacgg ggtgctctgt ctggagttcc actccaaggg
     2161 caactccata gacggcgcta tggtcgagat gggcaaccgg gcgctcgcgg agctggagcg
//
LOCUS       DASSMB010000174         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_548755, whole genome shotgun sequence.
ACCESSION   DASSMB010000174 DASSMB010000000
VERSION     DASSMB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548755"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1546)
                     /gene="gatA"
                     /locus_tag="VFJ72_10290"
     CDS             complement(101..1546)
                     /gene="gatA"
                     /locus_tag="VFJ72_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET7479887.1"
                     /translation="MADLLSLTAHELAKEIESGEVSAREAAEVANARVEEVEGEVNAF
                     VTVTPEIALERAGRVDAKNGSRKLWEGVPMAVKDVLSTRGVRTTCGSKILEDFTPLYD
                     ASALRNFGDDLIMVGKANMDEFAMGSSTENSAYGVTRNPWDLSRVPGGSSGGSAAAVA
                     AGEGWWALGTDTGGSVRQPAALCGIVGLKPTYGRVSRYGLIAFASSLDQIGPMTRDVR
                     DSALLLQAIAGHDSRDSTSANVEVPDYLSELENGVSGLRVGVVTELVNGEGIEPGVRE
                     VTERIAQGLEDAGAEVGEASLPHSEYALPAYYIIAPAEVSSNLARFDGVRYGLRVPAD
                     GVHEMYRETREAGFGDEAKRRIMLGTYALSSGYYDAYYAQAQKVRTRIIEDFRAAFAR
                     YDVLLSPTSPTVAFGIGAKVDDPLAMYYQDVCSIPANLAGIPAISVPGGLSEGLPVGV
                     QLMSDHFTEPTLLRAARAIEQAADFEFTLKP"
     gene            complement(1546..1827)
                     /gene="gatC"
                     /locus_tag="VFJ72_10295"
     CDS             complement(1546..1827)
                     /gene="gatC"
                     /locus_tag="VFJ72_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET7479888.1"
                     /translation="MISEEQVRHVANLARLGLKDGEVARMGEQLGAILESIDKIGELD
                     LDGVPPTANPLNLTNVLRPDEPREELPRDEALAPAPEPVDNLFAVPRID"
     gene            complement(1923..2159)
                     /locus_tag="VFJ72_10300"
     CDS             complement(1923..2159)
                     /locus_tag="VFJ72_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479889.1"
                     /translation="MFDRDIFKALIGEIAETREVEIGCDECFEQLDRFVNMKLSGLDP
                     GQALPLVQEHLEICGECRDEFEAFMAALRATEET"
     gene            complement(2249..2863)
                     /locus_tag="VFJ72_10305"
     CDS             complement(2249..2863)
                     /locus_tag="VFJ72_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7479890.1"
                     /translation="MAVPARERGNEQWLAELRGPARDRAIADLRLILLRGLRAALHGR
                     TSSIDPSLEDFAQEALIKILDNLDSFRGECRFTVWAQKIAVRTAFAEIRHSRWRDVSL
                     DEAIERQGHDEPADTLEDPSRFAAQAMIMTQFRCFIDEELTEKQRRALLAAMNGMPLE
                     AVADRMNTNRNALYKLLHDARKRLKKRMAAERLTPQDVLGAFGG"
BASE COUNT          470 a         1046 c          963 g          562 t
ORIGIN      
        1 gagtcggctt atgctgcggc gattggcgca tgcggcatgg gggtcatcta ccgcgttgtt
       61 gctcgggatt cgtcgtttac aaaactccct ttagcccgaa tcagggtttc agcgtgaact
      121 cgaagtccgc ggcctgctcg atggcgcggg cggcgcgcag gagcgtgggc tcggtgaagt
      181 ggtcgctcat tagctggacg ccgaccggga gcccttcgga gaggccgccg gggacgctaa
      241 tggcggggat tcctgcgagg ttcgcgggga tggagcagac gtcctggtag tacatcgcca
      301 gcgggtcgtc caccttcgcc ccgatcccga acgccaccgt cggcgaggtc ggcgagagga
      361 gcacgtcgta gcgcgcgaac gccgcgcgga agtcctcaat gatcctggtc cgcaccttct
      421 gcgcctgggc gtagtaggca tcgtagtagc cgctactcag agcgtaggtg ccgagcatga
      481 tgcggcgctt ggcctcgtcc ccgaagcccg cctcacgggt ctcgcggtac atctcgtgaa
      541 cgccgtccgc cgggacgcgc agaccgtaac gcacgccgtc gaagcgagcc aggttcgacg
      601 agacctccgc cggggcgatg atgtagtagg cgggcagggc gtactccgag tgcggcaggc
      661 tcgcctcccc cacctcggct ccggcgtcct ctaagccctg ggcgatccgc tccgtcacct
      721 cgcgcacgcc gggctctatc ccctcgccgt tcaccagctc ggtgacgacg ccgacccgca
      781 ggccggagac gccgttctca agctccgaga ggtagtccgg gacctcgacg ttcgcgctcg
      841 tggagtcgcg ggagtcgtgt ccggcgatgg cctgcaagag gagcgcggag tcgcgcacgt
      901 cgcgggtcat ggggccgatc tggtccaggg atgacgcgaa cgcgatgagc ccgtagcgcg
      961 agaccctccc gtacgtcggc ttgagcccca cgatgccgca cagcgccgcc ggctggcgca
     1021 cgctgccgcc cgtgtccgtg ccgagcgccc accacccctc gcccgccgcg accgccgccg
     1081 ccgagccgcc agaggagcca cccggcaccc gcgagaggtc ccacgggttg cgggtaacgc
     1141 cgtaggctga gttctctgtc gaggaaccca tcgcgaactc gtccatattc gccttgccga
     1201 ccatgatcag gtcgtccccg aagttcctga gcgccgaggc gtcgtagagc ggcgtgaagt
     1261 cctccaggat cttcgacccg cacgtggtcc gtaccccgcg cgtactgagc acgtccttca
     1321 cggccatcgg gacaccctcc cagagcttcc gagacccgtt cttcgcgtca acccgcccgg
     1381 cgcgctccag cgctatctcg ggcgttacgg tgacgaaggc gtttacctcc ccctcgacct
     1441 cctcgacgcg ggcgttggcg acctcggcgg cctcgcgggc ggagacctcg ccggactcta
     1501 tctccttcgc cagctcgtgg gcggtcaggc tcagtagatc tgccatcagt cgatcctcgg
     1561 cacggcgaag aggttgtcca cgggctcggg ggcgggtgcg agcgcctcgt cgcgcggcag
     1621 ttcctcgcgc ggctcgtccg ggcgcaggac gttcgtcagg ttgagggggt tcgcggtcgg
     1681 cggcacgccg tcgaggtcca gctccccgat cttgtcgatg ctctctaaaa tggccccgag
     1741 ctgctcgccc atccgggcca cctccccgtc cttcaggccc agccgggcca ggtttgccac
     1801 gtgacgcacc tgctcttcgg atatcatctc tcgcctccga ttctcgcgta aaaacctatc
     1861 ttagcgcacg acgcgccggg tattccggca gagtggagag ggtttcgcgg ggacagagcc
     1921 ggtcaggtct cttccgtcgc tcgcagggcg gccatgaacg cctcgaactc gtcgcggcac
     1981 tcgccgcaga tctccaggtg ctcctggacc aggggcaggg cctggccggg gtcgaggccg
     2041 gagagcttca tattcacgaa gcggtctagc tgctcgaagc actcgtcaca gccgatctcc
     2101 acctcgcggg tctcggcgat ctcaccgatc agggccttga atatgtcccg gtcgaacatt
     2161 tgcgttattc ttctccgtcc tccatagagg gatgtagaag gatacgacac accctcccaa
     2221 ccagtagatg cggtgagacc ttcagatctt accccccaaa agctccgagt acgtcttgcg
     2281 gtgtgagcct ctccgccgcc atcctcttct tcagccgctt gcgggcgtcg tggagcagct
     2341 tgtagagcgc gttccggttg gtgttcatgc ggtccgccac cgcctccagc ggcatgccgt
     2401 tcatcgcggc gagcaaggcc ctgcgttgtt tctccgtcag ctcctcgtcg atgaagcacc
     2461 gaaattgcgt catgatcatc gcctgggccg caaatcgcga ggggtcttcc agtgtatcag
     2521 ccggttcgtc atgtccctgg cgctcgatag cttcatccag cgagacatct cgccacctgc
     2581 tgtgccgtat ctcggcgaag gctgtgcgaa cggcgatctt ctgcgcccac accgtgaagc
     2641 ggcactctcc gcggaaggag tccaggttat ccagtatctt gatgagcgcc tcctgcgcga
     2701 agtcctccag tgagggatct atgctgctcg tcctcccgtg cagggcggcc ctcaatccgc
     2761 gcaagagtat gaggcgcagg tctgcgatgg ccctgtctct cgcgggcccc cgtagctctg
     2821 cgagccactg ctcgttgccg cgctctctcg cagggacagc cataaggtat attaccggcg
     2881 ttccgcgcca accgcgaccc cggatctcta cgacgctccg tatcgagcgg actcagcgag
     2941 agatatgctc gacgcgtgat ttcaccgcca tcacgaaggc gcgcacctcg gaggggtcca
     3001 ggcgtcgcgc ctggccgcga cggatctcca gtgccgcatc t
//
LOCUS       DASSMB010000175        28317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1436117, whole genome shotgun sequence.
ACCESSION   DASSMB010000175 DASSMB010000000
VERSION     DASSMB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 28317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28317
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436117"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VFJ72_10310"
     CDS             <1..1009
                     /locus_tag="VFJ72_10310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET7479891.1"
                     /translation="LMYGERDDPYAVISRLGERQETTDAPDEVLLVIVETIKEALRVP
                     YVAIKPRDDDAGGILAEVGEPIRETLRLPLTYQQAPVGELLIAPRAGSKGFSAADRRL
                     LEDLARQAGVAVHSARLTRDLQRSREKLVTAREEERRRLRRDLHDGLGAQLAGPNIQT
                     GVLRRLIPRDPAAADELAAELKTELQSAIADIRLLVYDLRPPALDDLGLVATLTQLAT
                     RYGAEDRGLRVTVEAPDNLPNLPAAVEVAVYRIAQEALTNVVRHARARTCVVRLAVDG
                     EVRFEVADDGAGVPEEHAAGVGLLSMRERAAELGGTCAVRPGPEGGTRVLVRLPLPGE
                     E"
     gene            1013..1666
                     /locus_tag="VFJ72_10315"
     CDS             1013..1666
                     /locus_tag="VFJ72_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479892.1"
                     /translation="METLRILVAEDHPLFRKGVTSLLSSIPDFEVVGEAESGEDAVAR
                     VEELQPDVVLMDLQMPGRGGIEATREILHDSPSVRVLVVTLFKDDDSVFLALRAGARG
                     YVLKDADEEELVRAIRAVGRGEAIFSPAVAERVLSYFAAPNPAPPQVFPTLTDREREI
                     LTLIAQGHPNPSIAKHLSLSTKTIGNYVSNIFTKLQVADRAQAIIRAREAGLGQDRG"
     gene            1917..2249
                     /locus_tag="VFJ72_10320"
     CDS             1917..2249
                     /locus_tag="VFJ72_10320"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HET7479893.1"
                     /translation="MRLLLDENVDRRLKRFFGEEHEVLTVADRGWKGKRNGELLNLAQ
                     RDFDVLLTTDRNIPYQQNISRLDLAVVILEVKRNTLEDLTPLMERVNTSLAETNPGNV
                     IRISLSGE"
     gene            2602..2832
                     /locus_tag="VFJ72_10325"
     CDS             2602..2832
                     /locus_tag="VFJ72_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479894.1"
                     /translation="MGVVGLLAAAGLWQLRRWGLLLTIIVSVLNILLAAPGILAEPHA
                     TGKLLAGVTTVGFALVIVLVMRSDSRRAFAAT"
     gene            2940..3437
                     /locus_tag="VFJ72_10330"
     CDS             2940..3437
                     /locus_tag="VFJ72_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479895.1"
                     /translation="MKNTSTAGTPAWLWSNLAAMGFSLEHTVADLAIIFGPLSSGFGV
                     PQALLSVMIGVLYTWWAWAFAKAVGGAKSWLVGLMAFNVLWVGGNGLTIFTCPPPCSL
                     VPVYADALHLGNLMLAPLATYFAFRAMRRISGPGSWLVMVGNIIIMLALVIAIIAVLT
                     SLAGA"
     gene            3576..4109
                     /locus_tag="VFJ72_10335"
     CDS             3576..4109
                     /locus_tag="VFJ72_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479896.1"
                     /translation="MSSSDLIRWGGLAAMVGGVVWVVDSALPLLATPESGVTDVLFII
                     AALCTVGGFAGFHALQKDDYRRIERGGFWTLVLATLALVVGLILSLLGNTALERLELF
                     PVGTFAVFVGFVLYGAATFQARVLPRWCGMGLIAGPFMFLLGNVGGIAFGLLCLALGY
                     TLWSRGNTSVGQPSRIS"
     gene            4224..4862
                     /locus_tag="VFJ72_10340"
     CDS             4224..4862
                     /locus_tag="VFJ72_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479897.1"
                     /translation="MSMSNTRRTAYFAYAAGVPGILANLSLIVMYVLLGLQSGNPEDQ
                     TLIGSAFHVAGSGSDLLGSLSTAFMIPVALFLGKRLPRKRTTRFTQAAGLVAMALLTI
                     GGPLLVLGVLTFEVDTPIAIGALIVLAIWLLLVNRWLHLLDALSYRVVRLGELFGAGF
                     LAGYAIVGLGLLLPWMSWPQLVVFGFGVLVGLPAYFGIPVWFVLLGRHLGKS"
     gene            5008..7128
                     /locus_tag="VFJ72_10345"
     CDS             5008..7128
                     /locus_tag="VFJ72_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7479898.1"
                     /translation="MTESTKEQGRGAGGMVRRAVAWLAWSLVALSVALLVGGIALART
                     AGSGAAVELTYGSEAELDSAVITLATVLIFSVVGAIIASRHPRNTIGWIFCGMGLLMG
                     LNVLAHGYVESWLASGSGIRSLAETAAWFGSWSYILLVFVSTSFMLLLFPDGRLPSPR
                     WRPVAWSAGLGIAGFVLGSATEAGPLEDFPQIANPYGFDSPVLGAVGLAGALVAGGSM
                     VASVISLIVRMRRAGSKQRQQIKWLAYGGAVVVGTIFVGGVISIWKGDFGIAVIAIAL
                     LGLPIFTGIAIVKHRLYDIDLIINRTLVYGALTASVVGMYVFVVGYLGVLFQTGGNFF
                     VSLLATGIVAILFQPLRDRLQRAVNHLMYGERDDPYNVLSRLGQRLETVLAPDAVLPT
                     IAETVREALKLPYAAVTLPRDGRFEVSAASGEPPAEPLRLPLSYQGETVGELLLAPRA
                     LGEEFSAADRRLLNDLAHHAGVAVHGVRVMADLRRSRERLVLAREEERRRLRRDLHDE
                     LAPTLAALGLAAATVGELIPADPKKAAALNAELQSEIRSTVGEVRRLVYDLRPPTLDE
                     LGLAEAIRQRAARYNASEESGGLQITVESPGLPPELPAAVEVAAYRIVQEALMNVVRH
                     AEASSCAIRLACPEDRALEVEVTDNGVGLPESPEFGVGLRSMRERAAELGGSCVAENA
                     APAGTRVFARLPLAEPAVRSEKEE"
     gene            7128..7769
                     /locus_tag="VFJ72_10350"
     CDS             7128..7769
                     /locus_tag="VFJ72_10350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479899.1"
                     /translation="MEPLRVLIADDHTLFRYGMQGLLSTQPDIEVVGEAATGEEAVAL
                     AGELEPDVVLMDIKMPGVSGIEATRRILEDHPHVRILMVTMFEDDASVFTAMRAGARG
                     YVLKDAAKDDVLGAIRAVGRGGAVFSPGVAARLMDFFTTSRPAVPREAFPTLTERERE
                     MLHLMAKGSSNAEISHLLSLSAKTVANYVSNILHKLQVADRKEAVIRAREAGL"
     gene            complement(7798..8724)
                     /locus_tag="VFJ72_10355"
     CDS             complement(7798..8724)
                     /locus_tag="VFJ72_10355"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET7479900.1"
                     /translation="MPDVAGGSVAEQAFPSLPLEAWEESKETLHRYAQVVGKVRLRYT
                     PFMSHWWHVTLYVNSRGLTTGPIPYGPIAFEISFDLIDNRLVVTTSDGDAFAFSLDDL
                     PVAEFYRRLFDGLRALGIDASINTKPFDLDDEQPLDKNVLHCVCDREYVRRYWRMLVQ
                     VDQAFKQFAGRFTGKQSPVHLFWHSFDLAVTRFSDRRIEQPESVDPVTREAYSHEVIS
                     FGFWPGDRNVREPTFYSYTAPEPDGLTDQPLEPEAASWLPEGGTALLPYEAVRSSESP
                     RETLLAFMESAYRAGAKTAGWDVEALRSGAAV"
     gene            complement(8782..10311)
                     /gene="asnB"
                     /locus_tag="VFJ72_10360"
     CDS             complement(8782..10311)
                     /gene="asnB"
                     /locus_tag="VFJ72_10360"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HET7479901.1"
                     /translation="MCGIIGGYGGMDPETAKRMMRRISHRGPDDSGKVEVAGNVLGHR
                     RLSIVDVEGGKQPLVTPDGKLYLVGNGEVYNHEEIRTTLRSSGLSTRSDNEVALRLVA
                     ERGPDALSGLLGMYAFLIAGEDGTFIAVRDPVGIKPLYWARRDGRTLFASEMRSFDEE
                     WQPDVESFPPGHYWTPEDGLVRFAAAVPEEENLEQIEGPSEPGAPIPDTILKKVRERL
                     IRTVERQMMGDVPVGVFLSGGLDSSLVAAIAARWYEKRGEKLKTFAVGLEDSPDLLAA
                     RAVAEHLDTEHHESVYTGDDALRVLPEVVRTIESFDPSLVRSAVPNYILAEFTAKHVK
                     VVLTGEGADEIFAGYEYLEDFRTEKDLHAELVRTIEGLHDLNLQRCDRVTMAHGLEAR
                     VPFLELDMISLGLSLPAGWKLAGEGQPEKRLLRQAFDGWLPDDFLWRKKAQFGDGSGA
                     ASVLKRRMEESVTEEEFENERYEVDPPLRTREETAYYRIFAEDLGDIRPKRTISRFAT
                     A"
     gene            10514..11422
                     /locus_tag="VFJ72_10365"
     CDS             10514..11422
                     /locus_tag="VFJ72_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7479902.1"
                     /translation="MARIIRGDFPNGERGVGRGVRRGGELGLEPSAVACLNLTPRPGD
                     VSGNLLMAGREVRAALRREPSLRWVVLPELVTCGYSDLESVHRFAEDADTGESARFFA
                     GLARELGIYIAYGFPEVVAGTAARVYDSANLVGPEGVLATYRKRNLVGTTPEHYVFRA
                     GMDLPVVEAGGMLVALVICWDLGFPETAREAAMGGADLILAPAAWREPWGPQYGLSCA
                     ARALDSGVYVASANQLGLYPEARFGTPGHLYGPDGLRVSRSLDRMSLGVMDPAAPELW
                     RALYGGTLMENEKVASIDEEPVEICS"
     gene            11528..14365
                     /locus_tag="VFJ72_10370"
     CDS             11528..14365
                     /locus_tag="VFJ72_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7479903.1"
                     /translation="MLHDALDGKLDGYLRTDEASRALWSTDASIYLRKPAGVVVARSE
                     EDVKLALAAARKAGLSITPRGTGTSLAGQATAPGLALDVSEMREAVRIDLEGRSCVVE
                     PGLVQGELNAMVEPHGLVFGADTSTSDVATLGGMIGNNSAGMRSLVFGTTADQVLGLR
                     CVLASEETVKLRPLPRAEAERRAGGDDAEARLLRGAMEIGDRYSREIKRRFPRMIRRV
                     SGYGLDALIDPEIVDLTRLVCGSEGTLAVVTRAELLLRELPEARALASFQFDSLTHAA
                     RATVELLEEGPSAVELLDDVAIGRARENSAYRDSTRFVQGDPKAVLLVEWSGTEEELD
                     ERFGKLDDLAQAVGSPVAVPLRSKTAMAQTVKLRKSTLPLLLGTSDKEKPVAFVEDAA
                     VPPERLEEFVRRFEEIVSENGTWACFYGHASVGTLHVRPALDTSDPEGVARMRRIAEE
                     VAELVVDCGGSLSGEHGDGLSRSEFLEGMYGPEIMRAFAEVKRLFDPQGMLNPGIIVD
                     PPPMDTQLRIGPGRERLPIDTNLDFSEQGGFAKAVELCNGSGFCRKRTGGTMCPSYMV
                     TLDEQDTTRARANMLRSVIEGTLPPEELTGKRMKEVMDLCVGCKACKTECPSRVDVAS
                     MKTEVLYQTGKKHGFSLRQKAAGNIRKQLALASRLPSLYNGVAGARLSRRAAALFGID
                     PRRELPKVTDRPFSKRFPDLPQGEGPAEVALFNDTWNEYQRPEVGEGAVRLFAAAGAR
                     VHLPEVVCCGRPMLSEGLVDEARENARRNLEILAPMLEWGIPLVGLEPSCILTMRDDY
                     RKLLPDDPRVEKLEAATRLFEEALLELVDDGAELPLEEGAPVLLHGHCHQKALVGTGP
                     TERILGLAADVEVVDSGCCGMAGLFGYEKGHYEVSMKMGERRLFPAVRSAEERTVVAP
                     GTSCREQILGGTGRQALHPAEYLASCLEDRA"
     gene            14358..15164
                     /gene="nudC"
                     /locus_tag="VFJ72_10375"
     CDS             14358..15164
                     /gene="nudC"
                     /locus_tag="VFJ72_10375"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF001299.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HET7479904.1"
                     /translation="MPERGSEEALWFAFRDEQLLVLDGDPVRVPDSSTFEELGLEAAF
                     RWEIGTLNGARCWAVEIDSDGGTPDGMVLRDLRSLFAGIDEGFFKMAGKAKQVVGWHT
                     THRFCGRDGTETGPVAGEMAKRCPRCGMIYYPRLSPAIIVLIRNGDHVLLARSPGFPP
                     GIYSVLAGFVEPGEAIEEAIKREVREEAGIEVENIRYFGSQPWPFPNSLMIGFTAEYA
                     GGELRIDPTEVEDAGWYTPDRMPPLPPRFSIARSMIEDFLDRHGSNPEGR"
     gene            15294..17552
                     /locus_tag="VFJ72_10380"
     CDS             15294..17552
                     /locus_tag="VFJ72_10380"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET7479905.1"
                     /translation="MGAFRLAALVVLAAALSAFAAYLVWGDTGPFSGSLVRVSNDAPG
                     HYRVGRFLVDVKGEGNEPSGVALSIAHGSQPHRVLWSSIPGESFVSAAKGEESVSQSR
                     AHFHVEDEISSLHEDQTIDSIKKDGDRLVIEGRLLDGGADGVRYELAFSQASDGRLRF
                     QVKVDEPSYDRVFLTYATSPEERFFGFGVQYTYFDMKGREVPIFIQEQGIGRGAQPIT
                     LGADWQAGAGGTPFTSYASVPHYITSEMRSLFLENYEYSDFDLRDGDRVQIEVFSPQM
                     TGQLLSGDTPKELIEQYTEYSGRMRPLPGWILSGAVIGLQGGTDTARKRYAELKALDT
                     PVAALWLQDWVGQRETSFGTQLWWNWELDKDHYPGWSKLVSDLKRDDVRVMTYVSPFL
                     ANVADKPHYRRDLFEEARRKGYLVENAKGEPYMIEISDFSAALVDFTNPKARAWYEDI
                     IEKNLIDEGVSGWMADFGEGLPYDAALDSGADPKSYHNRYAEEWAKVNREAIERAGHE
                     DDIVFFNRSGYTRSPEYATLFWLGDQLVSWDKYDGIKSAVTGLLSSGLSGYSLEHTDI
                     GGYTAIDSPLKDYHRSKELLMRWTEMAAFTAVFRTHEGNKPEDNYQFYSDRETLEHFS
                     RFAKVYAAWKPYREKLVREAAASGMPVVRHPFLEYPEDPEVYGLEYQFMVGSEFMVAP
                     VLDPGEETVEVYLPAGRWVHLWTGKRYGSRGKGVHVTVDAPIGEPAVFFKEGSSAGRD
                     FREELRRQELLR"
     gene            complement(17632..19197)
                     /locus_tag="VFJ72_10385"
     CDS             complement(17632..19197)
                     /locus_tag="VFJ72_10385"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7479906.1"
                     /translation="MRYALSSPAERYLERRIGPGVPQEDVCLAEISPPPSRLPDHPLV
                     ERVPETRVRYSRGESFPDLLALRSGRIEAFPDGVAFPTEETEVLDLLSHARRIGARVI
                     PWGGGTSVVGGVNPGLDEHPTLTVSLERMGRLRDLDEESMLATFESGVRGPWLEAQLR
                     GWGYTLGHFPQSFEYSTLGGWVATRSSGQQSLHYGRIEDLFRGGRLLTPEGPMDLPPF
                     PASAAGPDLRHVVLGSEGRLGLITEVQVRVHPLPEEERFWGIVFPGWEDGIEAVRIMA
                     QDPRPLSMLRLSDAEETNSLLHQALNPYVRRLLEGLLARRGLREEKSLLILGATGDSR
                     TVRGARARAAEISKARKGVAIGDVAGREWRNNRFRVPYLRNTLWEKGWAVDTLETATV
                     WSAVVPTARDVLRALRSTLDGERVLAMAHLSHVYPTGASLYFTFFFWLAADPESTLER
                     WRALKAAASRAIVARGATISHQHGVGADHLPYLEKEKGARGVAMLEALARAVDPDATM
                     NPGKLIPNPSDPL"
     gene            complement(19278..20885)
                     /locus_tag="VFJ72_10390"
     CDS             complement(19278..20885)
                     /locus_tag="VFJ72_10390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET7479907.1"
                     /translation="MPDFGEGYRGSTWDEAASRVWDVVVIGGGITGAGVAKETARLGL
                     SVLLVEQRDFAWGTSSRSSKMVHGGLRYLRERQIQLTREAVRERESLLRTGKGLVEPL
                     RFLYVVYQGDRPGPWMLDFGLSVYDLLKKGGKNHHGLDALDVWLSAPNLDLVGLRRGF
                     AYLDAQTDDARLVFRVLREAMSFGARALNYARADELLRDREGNVRAVAVSDADTGSVT
                     EVKTRFVVNATGVWSAEIVAGGGAPRLRPLRGSHLVLPREKLPVAQAITFAHPRDGRP
                     VFSYPWEGVTLVGTTDLDHDQPLRLEPAATREESDYLLEAVRHRFKDIHVSPEDLLGT
                     FAGVRPVIFGGRDHPSQEPREHAIWRDKNYLAIAGGKLTTFHPMAREAVRTVAADLGR
                     RSNGSASGGLEPLQRKIIERANETLSPLPHLLRRRLAGRLGPDLEAFLEWMREDDLQQ
                     IADTPYTWAELRWAFEREAVVHLEDLLLRRVRLGHLLPGGGRTLGGEMEARLKDLPGW
                     DPGRWEAEWRRYLKLWETHYSPEPAGS"
     gene            21171..21845
                     /locus_tag="VFJ72_10395"
     CDS             21171..21845
                     /locus_tag="VFJ72_10395"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479908.1"
                     /translation="MDVECVIRVAIVDDQRLFATGLARIIGAQPDMEVVGEAHTGEEG
                     VSLCAEVEPDVVLMDLSMPVMNGVSATRKIRTILPRTRVLILTVHTDDAHVFQGIKAG
                     AQGYILKDCTPEDLTRAIRTVYAGDTIMAQDIARKMLTTFEGIRANNADIAPHLTERE
                     LDVVKALARGMSNKEIAQTMNISEKTVRNHASNIYKKLHIFDRTQAVLYAIRKGLVDI
                     EDLEPG"
     gene            complement(21868..23313)
                     /locus_tag="VFJ72_10400"
     CDS             complement(21868..23313)
                     /locus_tag="VFJ72_10400"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7479909.1"
                     /translation="MREETLEIGLGRGWRAGSTWRKSELQFRRLLEKLPAGAYTCDPD
                     GLITYFNQHAVKLWGRAPKLNDPVDRYCGSFKLYSTDGPPISHDQCWMALALRTDREY
                     NGREIVVERPDGERLTALAHANPIHDESGKLLGAVNVLVDITDRKRAEEKVRFQAHLL
                     EQVQTAVIATDMQGEVIHWNEYAEKLYGWSREEAVGRNISELTIGPKEIRVAEEIMIQ
                     LRAGKSWEGEFVTRRKDGSSFPAYVTDSLVHDDQGNAIGIVGVSTDITERKRLEESLY
                     EIREAERRRIARDLHDVVLQDLAGALQGMQAARIEARRAGAEIGLEQEIDALRRASKG
                     LRNAIYNLRLEGRQTFVRAVESLVELNSQMTPEREITLTLQDGFPQELPECVSVEMLR
                     VLQEALSNARRHSGASRVEVALSMEGDLLRAEIRDDGRGFDPHSARIGLGISGMRERA
                     SALGGELEIEGHPGRGVGIRVEIPIPESADV"
     gene            23679..23987
                     /locus_tag="VFJ72_10405"
     CDS             23679..23987
                     /locus_tag="VFJ72_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479910.1"
                     /translation="MRVIEREKEHYEVQEVPYGKVYSWRPERILFECDCGETLAWMVP
                     VTVCRCGAKHTGIALKEGPPPDEHTRHPWLAEYEEWRKVKLANDLRHEYYGFLKAEDE
                     "
     gene            23984..24382
                     /locus_tag="VFJ72_10410"
     CDS             23984..24382
                     /locus_tag="VFJ72_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479911.1"
                     /translation="MIRGVETKTEQAPSIGEHSVAVWYLISRCEHNRLDILTVFPRDD
                     EETVPVFGTRRMAEEFLRDGGFEQGWRVRESTAGELISLLLSCLVDLNSVSMDPSPDL
                     AFTGTAPSKRATRREFIAALMGRPLLVPVP"
     gene            complement(24420..25454)
                     /locus_tag="VFJ72_10415"
     CDS             complement(24420..25454)
                     /locus_tag="VFJ72_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET7479912.1"
                     /translation="MAQSAVAASAAYFVAVWVLGHERPFFASIAAVISLSVTLGQRGR
                     RAVEIVFGVAVGLLVADFIVLLIGTGTLQIGLVVALAMGAAVFFGGGTLLVNQAAISA
                     LLVVILQPPDSFFSPTRFFDALIGGSVALAIHYLFPANPERLVERAAAPVFEELVTVL
                     EEVAAALAGGDADRARRALLRARELDVRVRRFNEALAAGHETARLSPTRRRALQHLEL
                     YANAGLRIDLAVINARVLARGAANATRRGGAAPPRLPEAVLDLSRAVKALETFLEESG
                     GYEEARRYALGAAQKATEILSDRHDLATSVLVGQIRSAAVDLLRSTGMDQESALQALE
                     EAAGRASEIA"
     gene            25810..26421
                     /locus_tag="VFJ72_10420"
     CDS             25810..26421
                     /locus_tag="VFJ72_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7479913.1"
                     /translation="MAVEGGRRRERRDAVESRRKVLGAARRLFEERGVDAVSMHEIGR
                     AAGVGQGTLYRRFEHKGALCSALLQEEIEGFEEEARRRVGSRGSALGRLKWFLGRLAR
                     FNDENAALLGAVRDAAGGERRIETHRNPFYEGLRENVAELLWEALEEGEIPEELDIEC
                     MTDTLLAALGIDLYLYQRRELKMSRERIVDSLRSLLEALRVRG"
     gene            26509..27003
                     /locus_tag="VFJ72_10425"
     CDS             26509..27003
                     /locus_tag="VFJ72_10425"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET7479914.1"
                     /translation="MGRQRVKPGPGQESVWDYPRPPRMEDVDKKVKVVFGGVTIAYTT
                     RAKRVLETSHPPVYYIPPEDIRMEHLVPADGSSMCEWKGRARYYDVVTEDRTERRAAW
                     YYPEPVWSFSGLKGYVAFYPSRMDGCWVGGEKVEAQEGDFYGGWISPEIVGPFKGAPG
                     TWGW"
     gene            27014..27652
                     /gene="pdxH"
                     /locus_tag="VFJ72_10430"
     CDS             27014..27652
                     /gene="pdxH"
                     /locus_tag="VFJ72_10430"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041328297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET7479915.1"
                     /translation="MDPVARLRREYTRTGLRESDVDEDPIEQFRRWFEEVMEANLHEP
                     NAMTVATSTADGKPSARIVLLKGFDERGFVFYTNYEGRKSREIETNPRAALLFYWGEL
                     ERQVRIEGRVERIAETDSDAYFASRPRGSQLGAWASEQSRPVEDRGALEERLDEVEER
                     FGEGDIPRPPFWGGYRVEPESIEFWQGRESRLHDRILYRRSGSGWKIERLQP"
     gene            27742..>28317
                     /locus_tag="VFJ72_10435"
     CDS             27742..>28317
                     /locus_tag="VFJ72_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7479916.1"
                     /translation="MKLVTYSTDDGVPRVGALEGEEIRALGGAGSDMIEFIERGDSPE
                     PGGETVALSSARLHAPVLTPQKVIGIGLNYEDHAAETGADIPEKPIVFAKYPNSIIGP
                     GEEIVIPQITEQADYEAELAVVIGRAARNVSESEALDYVFGYTNCNDVSSRDLQFSEG
                     GQWTRSKSIDTFCPIGPYIATRDEIEDPQDLA"
BASE COUNT         4822 a         8810 c         9636 g         5049 t
ORIGIN      
        1 cctgatgtac ggagagcgcg acgacccgta tgctgtgatc tcacgcctcg gggagcgcca
       61 ggagacgacg gacgctcctg acgaggtgct gttggttata gtggagacga tcaaggaggc
      121 gctccgggtg ccatacgtgg cgatcaagcc cagagatgac gatgccgggg ggatcttggc
      181 ggaagtcggg gagccgatca gggagacgct gcgtctgccg cttacgtatc agcaagcgcc
      241 ggtcggagag ttgctgatcg cgcctcgcgc cgggagcaag gggttctccg ccgccgatag
      301 gcgactgctg gaagacctgg cccggcaggc cggggtcgcc gtacattccg cgcgcctcac
      361 gagagacctg cagcgttcgc gtgagaagct ggtcaccgcc cgcgaggagg agcgccgccg
      421 cctgcggcga gatctgcacg acggcctggg cgcccagcta gccggtccca acatccagac
      481 tggggtgctg cggcgcctga tcccccggga ccccgcagcc gctgatgagc tggcggccga
      541 actgaagacc gagctacagt ccgccatcgc ggacatccgg ctgctggtat atgacctccg
      601 gcctccggcc ctggacgacc tgggacttgt cgccaccctg acccagctag ccacgcgata
      661 cggcgcggag gacagagggt tgcgggtgac agtcgaagcg ccggacaatc tgcccaacct
      721 ccccgccgcc gtcgaggtgg ccgtctaccg catcgcgcag gaggccctga cgaacgtggt
      781 ccgccacgcc cgcgcgcgga cgtgcgtggt gcggctcgcg gtggatggcg aggttaggtt
      841 cgaggtcgcg gacgacgggg ccggggtgcc ggaggagcac gcggcaggcg tcggtctgct
      901 ctcgatgcgc gagagagcgg cggagttggg cggtacttgc gcggttcggc ccgggccgga
      961 gggcgggacg agggtgctag tccggttacc gttgcccgga gaggagtgag aggtggagac
     1021 gctgcggata ctggtagccg aggaccaccc gctatttagg aagggtgtga cctccttgct
     1081 ctcctcgatc ccggacttcg aggtcgttgg agaggccgag agtggtgaag atgccgtcgc
     1141 gcgcgtggag gagcttcagc cggacgtggt cctcatggac cttcagatgc ctggccgggg
     1201 cggcatagag gcgacccgcg agatcctgca cgacagtccc agcgtccgcg tcctggtcgt
     1261 cacactcttc aaggacgatg actcggtctt cctggccttg cgagccgggg cgcgcggata
     1321 cgtcctcaag gacgccgacg aggaggaact ggtgcgtgcg atccgcgcgg tgggacgcgg
     1381 ggaggccatc ttcagcccgg cggtcgcgga gcgcgtgctt tcctacttcg ccgctccgaa
     1441 tcccgcgccg ccgcaggtct ttccgacgct caccgatcgc gagcgcgaga tcctgaccct
     1501 gatcgcccag ggccacccca acccatccat agccaaacac ctgtctctca gcacaaagac
     1561 catcggcaac tacgtctcca atatcttcac caagctccag gtcgccgacc gcgcccaggc
     1621 catcatccgc gctcgtgagg ccggtctggg ccaggatcgc gggtagcgga caaccccgtg
     1681 cgaaggtggg tagtagaata tagccgtgaa ccgaggagac atcatttccc gaaaccccga
     1741 ggtgatgagc ggtgagctgg ttttcacagg gacccgcgtg ccggttcaga ctctcatcga
     1801 ttacctggag gccggacatt cgctagacga ctttctcgac ggtttcccta ccgtgagccg
     1861 tgagcaggca gtctcctacc tggagataac actagaggct gccgaggttc tcgcgagtgc
     1921 gccttctgct cgatgagaac gttgataggc gtctaaaacg gttctttggc gaggagcacg
     1981 aggtcctgac cgtcgcggat cgcggctgga aaggcaagag aaacggtgag cttctgaacc
     2041 tcgcgcagcg cgatttcgac gtgctattga ccaccgaccg taacatacct tatcaacaga
     2101 acatatcacg gctggatctt gcagtcgtca tacttgaggt gaagaggaac actcttgagg
     2161 atctcacgcc actcatggag cgggtaaata cctcgctcgc cgagacgaat ccaggtaacg
     2221 tgattcgcat ctctctttca ggcgaatgac ctacaacatc ttcactaggc tccaggtcgc
     2281 cgaccgcgcc caggccatca tccgcgctcg tgaagccggt ctcggacgaa acactagccg
     2341 ggacctgtaa agtcgggcga aagctcccac caaaattccc ggactttggg aagaccttcc
     2401 cctacctccc gggaaaggcc cccggctaga gtgacgctat ggagtccaaa aactcaccaa
     2461 ggaggaagag ctgtgaacac taaacgaccg ctgcccctgg cagtcggtgt cgtcctgctg
     2521 gcactgttta gcctgcttaa ttttgtatcc tcgttgttgc caacggatta cccgacgacc
     2581 gtccttttgt tgggcggtgc gttgggcgtc gttggcctcc tcgccgccgc cggtctgtgg
     2641 cagttgagaa ggtggggtct gttgctgacc atcattgtat ccgtgctcaa catactgttg
     2701 gcggctccag ggatactcgc cgagccacac gccacgggca aactcctcgc aggggttacg
     2761 accgtgggct tcgctctcgt catcgtgctg gtgatgcggt cggactcgcg acgcgctttc
     2821 gcggctacct gagtttccga gaccagtgcc agctacccgc gaccactatc ctacgcctgc
     2881 ttttgggcgc aaggagacgc ggcagtgttc acacaaaata caatgaagga gtcacatcca
     2941 tgaagaacac ttccacagcg ggcacgccgg cttggctctg gtccaacctc gccgcgatgg
     3001 ggttcagcct ggagcatacc gtggcggact tggcgattat cttcggaccg ttgtcatcag
     3061 gcttcggcgt gccgcaggct ttgcttagtg tgatgatcgg agtactgtac acttggtggg
     3121 cttgggcttt cgcaaaggct gttgggggag cgaagtcttg gctggttggc ctgatggcgt
     3181 tcaatgtcct ctgggttggc ggcaatggcc tgacgatctt tacctgcccg cccccctgta
     3241 gcttggtgcc agtatatgcc gacgctctac acttaggcaa tcttatgctt gctccgctgg
     3301 cgacttactt cgccttcagg gccatgagac gaatttcagg gccgggcagc tggctggtaa
     3361 tggtgggcaa cattatcatc atgctggcct tagtgatagc gattattgca gtgctgacat
     3421 cgcttgcggg cgcctgagca gacttctgat ggtgagcaac gccggcggct cggcccaaac
     3481 gaggttgccg tgtccgaacg cggcctcggc cggcggtgtg ccgctaccga atattaacgg
     3541 cggcccgctc gccgaatacg aaaggagaaa atactatgtc gtcttcagac ctgattcgct
     3601 ggggtgggtt ggccgccatg gtcgggggtg tggtgtgggt tgtggactcg gcccttccac
     3661 tccttgctac gccagaaagc ggcgtgaccg acgtcctgtt cattatcgcg gcgctctgca
     3721 cagttggggg gtttgccggt ttccacgcgc tgcagaagga cgactacagg cgcattgaac
     3781 ggggcggctt ctggacgctc gtcctggcca cgctagccct ggtagtagga ctgatactct
     3841 cgttgctggg taacacggct cttgagaggc tcgagttgtt tccggtggga accttcgctg
     3901 tgtttgtcgg gttcgtgctc tacggagctg ccacctttca agcgagggtg ttaccccgct
     3961 ggtgcgggat ggggttgatt gctggtccgt tcatgttcct cctggggaac gtcggaggga
     4021 tagcgttcgg gcttctgtgc ctggcgctgg gatacacgct ctggtcgcgg gggaatacgt
     4081 ctgtggggca accctcgcgc ataagctgag tttccgagaa tatgagaagc ggtccaggcc
     4141 gcaagcaggc ggtgagggtg cggatgacga ggaggtagtt gaaggtcatg tccacgaaga
     4201 cctgtatagt agtagggcgt gcggtgagca tgtcgaacac caggaggaca gcctacttcg
     4261 cctacgcggc aggcgtcccc ggcattctcg ccaacctctc cctcatcgtc atgtacgtcc
     4321 tgcttgggct tcagagtgga aatcccgagg atcagaccct gatcggttct gccttccatg
     4381 tggcgggatc gggcagcgat cttttgggat cgctctcgac ggcgttcatg atcccggtcg
     4441 cgctattctt gggcaaacgc ctgccgcgca aacgaacaac acgcttcacg caggcggccg
     4501 gtctggtggc catggcgctg ttgaccatcg gtggtccgct gctggtgctc ggggtgctta
     4561 cgttcgaggt cgatacaccg atcgccatcg gggcgctaat cgttctggca atctggctat
     4621 tgctggtcaa ccgatggttg cacctgttgg atgctctttc gtaccgcgta gtaaggttgg
     4681 gagagttgtt tggcgcaggc ttcctggccg ggtacgcgat cgtggggcta ggtctcttgc
     4741 tgccgtggat gtcgtggccg cagctcgtgg tgttcggctt tggcgtcctc gtcgggctgc
     4801 ccgcctattt tggcatcccg gtgtggttcg tgctgctggg ccggcacctc gggaagtcat
     4861 gagcacgatc ggatggccga cggcgcccgg tcatctcggg tatccgaacc ttattcaccg
     4921 atgtgtttgg cgagggcttc tcggaagttc gctacggatc ttcttcctcg taacgtctgg
     4981 gagcgggtcg ccttgtagaa tggtgttgtg acggaaagca caaaagaaca gggccgagga
     5041 gccggtggga tggtccgtcg cgccgtcgcg tggcttgcct ggtcgctggt ggcgctatcc
     5101 gtggcgctgc ttgtgggcgg gatcgcgttg gctcgcacgg cggggtccgg cgccgcagtt
     5161 gagcttacgt acggcagtga ggcagagctt gatagcgcgg tcattaccct ggctacggtg
     5221 ctgatcttct cggtcgtagg cgccatcatc gcttcccgac acccccgcaa caccataggc
     5281 tggatcttct gcggcatggg cctgttaatg ggcctcaacg tactcgcgca cggctacgtt
     5341 gagtcctggc tcgccagcgg ttccggcata aggagcctcg ccgaaacggc ggcgtggttc
     5401 ggctcgtggt cgtatatcct cctggtgttc gtctcaacga gcttcatgct gctgctgttc
     5461 cccgacggtc ggctaccctc gcctcgctgg aggccagtgg cgtggtctgc gggacttggg
     5521 atcgcggggt tcgtcttggg ctccgcgacg gaggccgggc cactcgaaga cttcccccag
     5581 atcgcaaacc cctacggctt cgatagccct gttttgggtg cagtgggact tgcaggcgcc
     5641 cttgtggccg ggggctccat ggtagcgtcc gtcatctcgc tcatcgttcg gatgcgccgt
     5701 gccgggagca agcagcgcca gcagatcaaa tggctcgcat acggaggcgc ggtcgtggtt
     5761 ggcaccatct tcgtgggtgg cgttatctct atctggaaag gagacttcgg catcgcggtc
     5821 atcgccatcg ccctgctagg gttgccgatc ttcacgggca tagccatcgt caaacatcgc
     5881 ctctacgaca tagacctcat cataaaccgc accctcgtct atggcgctct aaccgcgagc
     5941 gtggtcggca tgtacgtgtt cgtcgtaggc tacctgggcg ttttgttcca gacaggcggc
     6001 aacttcttcg tctcgctcct ggctaccggg atcgtggcca tactattcca accgctgcgc
     6061 gaccggctgc agcgcgccgt caaccacctg atgtacggcg aacgcgacga tccctacaac
     6121 gtgctctcgc gcctcggcca gcgcttggag accgtcctcg ctcccgatgc cgtgctgccg
     6181 accatagcgg agaccgtccg ggaggcgctg aagctgccct acgccgcagt cacgctcccg
     6241 cgggacgggc ggttcgaggt ctccgccgcc tccggcgagc cgcctgccga gccgctgcgg
     6301 ttacccctct cttaccaggg tgagaccgtg ggcgagttgc tgctcgctcc gcgcgcgctg
     6361 ggcgaggagt tctccgccgc cgaccggcgc ttgctgaacg acctggcgca ccacgctggt
     6421 gtggccgtgc atggcgttcg cgtgatggcc gacctccggc gctcgcgcga gaggctggtg
     6481 ctggcgcgag aagaggagcg ccgccgcttg cgtcgggacc tgcacgatga actggcccca
     6541 acgctcgcgg cgcttgggct ggcagccgcg acggtggggg agttgatccc ggcagacccc
     6601 aagaaggccg ccgccctcaa cgcggagctc cagtcagaga tacggtccac cgtcggcgag
     6661 gtacgccgcc tcgtctacga cctccgtcca cccaccctgg acgaactcgg gctggcggag
     6721 gccatccggc aacgcgccgc caggtacaac gcatcggagg agagtggggg cctccagata
     6781 acagtcgaat ccccgggact cccgccggag ctgcccgctg cggtcgaagt cgccgcgtac
     6841 cggatcgttc aggaggcgct gatgaacgtc gtccgccatg ctgaagccag ttcgtgtgcc
     6901 atccggctgg cctgtcccga ggaccgggcg ctcgaagtag aagtcaccga taatggtgtc
     6961 gggctgccgg aatcgccgga gttcggggtc ggcctgcgct cgatgcggga acgcgccgcc
     7021 gagctgggag gtagttgcgt ggctgaaaat gccgccccgg ccgggactcg cgtcttcgcg
     7081 aggctgccgc tcgcggagcc cgccgttcgt agcgagaagg aggaatagtg gagcctctgc
     7141 gcgtcctgat cgccgacgac cacaccctct tccgctacgg gatgcagggc cttctctcca
     7201 cccagccgga catcgaggtc gtgggggagg cggcgacggg ggaggaggcc gtcgcgctgg
     7261 ccggcgagct tgagccggac gtggtgctaa tggacatcaa gatgccgggc gtgagcggca
     7321 tagaggccac ccgccgcatc ctggaggacc atccgcacgt ccgcatcctc atggtgacga
     7381 tgttcgagga cgacgcctcg gtcttcacgg cgatgcgggc cggggcgcga ggctatgtcc
     7441 tcaaggatgc cgcgaaggac gatgtgctcg gcgccatccg ggccgtcggc cgtggaggcg
     7501 cggtcttcag ccccggcgtc gcagcccggc ttatggactt cttcaccact tcgcgacccg
     7561 ccgtaccgcg ggaagccttc cccaccctga ccgagcgcga gcgagagatg ctgcacctca
     7621 tggccaaggg ctcgtcgaac gccgagatct cacacctcct ctccctgagc gccaagacgg
     7681 tagccaacta cgtctccaac atcctccaca agctgcaggt cgccgaccgg aaagaagccg
     7741 tcatccgcgc tcgcgaggcc gggctctaac gcaacagtct gcgagcggta tcttttctca
     7801 aacagcggca ccggacctga gcgcttccac gtcccagccc gccgtcttcg cgcccgcccg
     7861 gtaggcgctc tccatgaagg ccagtagcgt ctccctcggg gattcgctgc tccgcaccgc
     7921 ttcatatggc aggagcgcgg tgccgccttc ggggagccag gacgcggcct ccggctccag
     7981 cggctggtcg gtgaggccgt ccggttcggg ggcggtgtag gagtagaagg tgggttcgcg
     8041 gacgttgcgg tctccgggcc agaagccgaa ggagataacc tcgtgcgagt aggcctctcg
     8101 cgtaaccgga tccacgctct cgggctgctc gatgcgtcga tcggagaaac gggttacggc
     8161 caggtcgaag ctgtgccaga agaggtggac cgggctctgc ttcccggtaa accggccggc
     8221 gaactgcttg aaggcctggt ccacctgtac gagcatccgc cagtagcgcc ggacgtactc
     8281 ccggtcgcag acgcagtgga ggacgttctt gtcgagcggc tgctcgtcgt cgaggtcgaa
     8341 cggcttcgtg tttatggagg cgtcgatgcc cagggcgcgg aggccgtcga agagcctgcg
     8401 gtagaactcc gcgacgggca ggtcgtcgag ggagaaggcg aaggcgtcac cgtcgctcgt
     8461 cgtgacgacg agccggttgt cgatgaggtc gaaggagatc tcgaacgcga tgggtccgta
     8521 ggggatcgga cctgtggtaa gcccccggct gttgacgtag agcgtcacgt gccaccagtg
     8581 gctcatgaag ggcgtgtatc ggagccgcac cttgccgacg acctgggcgt aacggtgcag
     8641 cgtctccttg ctctcctccc acgcttcgag cggtagggac ggaaaagcct gctccgccac
     8701 ggagcctccg gccacgtccg gcatcgcgcc actcctctcc ttggacttaa cctgtcggca
     8761 tctctggagg gtattgtaat ctcaggcggt ggcgaaacgg ctaatggtgc gcttcgggcg
     8821 gatgtcgccg aggtcttcgg cgaagatgcg gtagtaggcg gtctcctcgc gggttcggag
     8881 aggaggatcc acctcgtagc gctcgttctc gaactcttcc tccgtgacgc tctcttccat
     8941 gcgccgcttg aggacggagg ccgcgccgga gccgtcgccg aactgcgcct tcttgcgcca
     9001 caggaaatcg tccgggagcc agccgtcgaa cgcctgacgg aggaggcgtt tctcgggctg
     9061 gccttctccg gcgagcttcc acccggcggg aagggagagt cccagagaga tcatgtccag
     9121 ttccaggaac gggacccgcg cctccagacc gtgcgccatc gtgactcggt cgcaccgctg
     9181 caggttcaaa tcgtgcagac cctctatggt ccggaccagc tcggcgtgaa ggtccttctc
     9241 cgtgcggaag tcttcaaggt actcgtagcc cgcgaagatc tcatcggcgc cctcgccggt
     9301 cagcacgacc ttgacgtgct tcgccgtgaa ctccgcgagg atgtagttcg ggacggcgct
     9361 ccgcaccagc gacgggtcga agctctcgat ggtacggacg acctccggca gcacccgcag
     9421 ggcgtcatcg ccggtgtaga cgctctcgtg gtgctcggtg tccaggtgct ccgccacggc
     9481 gcgggcggcc agcaggtccg gcgagtcttc gagcccgacg gcgaaggtct ttagcttctc
     9541 gccgcgcttc tcgtaccagc gggcggcgat ggcggcgacg aggcttgagt cgaggccgcc
     9601 ggagaggaag acgcccaccg gcacgtcacc catcatctgc cgctcgacgg tgcggattag
     9661 acgctcccgg actttcttca ggatagtgtc ggggatgggc gctccgggct cagacggacc
     9721 ttctatttgt tccaggtttt cctcctcggg gacggcggcg gcgaagcgga ctagaccatc
     9781 ttcgggggtc cagtagtggc cgggcgggaa ggactcgaca tcgggctgcc actcctcgtc
     9841 gaaggaccgc atctcggagg cgaagagcgt tctgccgtcg cggcgcgccc agtagagggg
     9901 ctttatgcct accgggtcgc ggacggcgat gaaggtcccg tcttcgccgg cgatgaggaa
     9961 ggcgtacatg ccgagcagac ccgagagcgc gtcggggcca cgctcggcga cgagccggag
    10021 cgcgacctcg ttgtccgagc gggtggagag gccgctgctc cggagagttg tccggatctc
    10081 ctcgtggttg tagacctcgc cgttgccgac caggtagagc ttcccgtccg gtgttacaag
    10141 gggttgtttg ccgccctcga cgtccacgat ggagagccgg cggtggccga ggacgttgcc
    10201 ggcgacctcg accttgccgg aatcgtccgg gccgcggtgg gagatgcgtc gcatcatgcg
    10261 cttggcggtc tccgggtcca tgcctccgta tccgccgatg attccgcaca ttgcgtttca
    10321 ctcctccggg ccgcgtcagg tcaggggtca tggtactcgc acgggcggag cgtgtcgatg
    10381 atccagccgt acatgacgac gcgctcgccg cgccggaatt atccgccatt tgtacgatgc
    10441 cggaagatat ggcggacgga tattatggcg atacgtctgg agggagcggt atcttttcga
    10501 aagggggcgg gccgtggcgc ggatcatccg gggcgacttc ccgaacggcg aacgtggcgt
    10561 cgggcgtggc gtcaggcgcg gcggcgagct ggggctcgaa ccctccgccg ttgcgtgcct
    10621 gaacctcacc cctcgtccgg gcgacgtgtc cggcaacctg ctgatggcgg gacgggaggt
    10681 ccgggcggcc ctgcgccggg aaccgtcgct gaggtgggtg gtcctgcccg agctagttac
    10741 ctgcgggtac tcggatttgg aatccgtcca tcggttcgcc gaggacgcgg atacgggaga
    10801 gagcgcccgg ttcttcgccg ggctggcgcg ggaactcggg atctacatcg cttacggatt
    10861 cccggaggtg gtcgcgggga ccgcggcgcg cgtctacgac agcgcgaacc tggtggggcc
    10921 ggagggggtg ctggcgacct atcgcaagcg caacctggtc gggacgacgc cggagcacta
    10981 tgtattccgt gcggggatgg atctcccggt cgtggaagcc ggaggaatgc tggtcgctct
    11041 cgtcatctgt tgggacctgg gttttcctga gaccgcgcgg gaggcggcga tgggcggcgc
    11101 ggacctcatc ctcgctcccg ccgcgtggcg cgaaccgtgg ggaccgcagt acggtctctc
    11161 ctgcgcggcg cgcgctctcg attccggcgt ctacgtcgcc agcgccaacc agctcggcct
    11221 gtatcccgag gcgcgcttcg gcactcccgg acacctctac ggtcccgatg gactgcgagt
    11281 ctcccgtagc ctcgaccgga tgagcctcgg tgtgatggac cccgccgccc ccgagctgtg
    11341 gcgcgcactc tatggcggca cgctgatgga gaacgaaaaa gtggcgtcca tcgatgagga
    11401 gccggtggag atctgctcct aatatagttc gccaaaacca tataaactgt ccccagtcca
    11461 tacggaaagg ggcgatgctg gtgaggcgcg ggtcatgatt ggcgaacgtt cggattctcg
    11521 aacacggatg ttgcacgatg ctctcgacgg caagctggac ggctacctcc gcaccgatga
    11581 agcctcccgc gccctctggt ccaccgacgc ttccatctac ctgcgcaagc ccgcgggcgt
    11641 cgtcgtcgcc cgctccgagg aggacgtgaa gctggccctc gcggcggcgc ggaaggccgg
    11701 gctctccatc acgccgcgcg gcaccgggac gagcctcgcc gggcaggcga ccgcgccggg
    11761 gctcgccctc gacgtctcgg agatgagaga ggccgtgcgg atagacctcg aaggccggag
    11821 ctgcgtggtc gagccggggc tcgtacaggg tgagttgaac gcgatggtcg agccgcacgg
    11881 gctggtgttc ggggcggata cgtccacctc ggacgtggcg actctgggcg gcatgatcgg
    11941 gaacaactcc gccggaatgc ggagcctcgt cttcgggacg accgcggatc aggtcctggg
    12001 actccgctgc gtcctcgcct ccgaggagac ggtcaaactt cgcccgctgc ctcgcgccga
    12061 ggcggagcgg cgcgcgggcg gcgatgatgc cgaggcgcgg ctcttgcggg gagcgatgga
    12121 gatcggggat cggtactccc gcgagataaa gcggcggttc ccgcgcatga tccggcgcgt
    12181 ctccggctac ggcctggacg ccctgataga cccggagata gtggacctca cccgcctcgt
    12241 ctgtggctcg gaggggacgc tggcggtagt cacccgcgcc gagctgctgc tgcgcgagct
    12301 gcccgaagcc cgcgccctcg cctccttcca gttcgactcc ctgacgcatg ccgcccgcgc
    12361 cacagtggag ttgctggagg aaggtccctc ggctgtggag ctgttagacg acgtggcgat
    12421 aggccgcgcc cgcgagaact ccgcctaccg cgactcgacc cgtttcgtgc agggagaccc
    12481 gaaggccgtg ctgctcgtgg agtggagcgg gaccgaagag gagctggacg aacggttcgg
    12541 caagctggat gatctcgccc aggcggtagg ttctccggta gcggtgccgc tgcgctcgaa
    12601 aacggcgatg gcgcagacgg tcaaactgcg gaagtccacg ctgccgctac tgctcggcac
    12661 ctcggacaag gagaagcccg tcgcgttcgt ggaggacgcg gcagtcccgc cggagcggct
    12721 ggaggagttc gtccggcgct tcgaggagat cgtctcggag aacgggacct gggcgtgctt
    12781 ctacggccac gcgagcgtcg gaaccctgca cgtccggccc gcgctggaca cgagcgaccc
    12841 ggagggcgtc gccaggatgc ggcgcatcgc ggaggaggtc gcggagctgg tggttgactg
    12901 tggagggtcg ctctccgggg agcacggaga tggactgtcg cggtcggagt tcctggaggg
    12961 gatgtacgga ccggagatca tgcgcgcctt cgccgaggtc aagcgtctct tcgacccgca
    13021 ggggatgctg aaccccggca ttatcgtgga cccgccgccg atggacaccc aactccgcat
    13081 cggccccggc cgcgagcgcc tgcccataga cacgaacctg gacttctcgg aacagggcgg
    13141 cttcgcgaag gccgtggagc tgtgcaacgg ctctgggttc tgccgcaaga ggaccggcgg
    13201 cacgatgtgc ccctcataca tggtcaccct ggacgagcag gacaccacgc gggcgcgggc
    13261 gaacatgctc cgctcggtga tcgaggggac gctgccgccg gaggaactga ccggcaagcg
    13321 catgaaggag gtgatggacc tctgcgtcgg gtgcaaggcg tgcaagaccg agtgcccctc
    13381 gcgggtggac gtggcgagca tgaagacgga ggtcctctac cagaccggca agaagcacgg
    13441 cttctccctg cgccagaagg ccgccgggaa catccgaaag caactcgccc tcgcctcccg
    13501 cctcccgtcc ctctacaacg gcgtcgccgg ggcgaggctc tcccgccgcg ccgccgctct
    13561 cttcggcata gatccgcgcc gcgagctgcc gaaggtgacg gaccggccgt tctcgaagcg
    13621 cttcccggac ctgccgcagg gagagggacc ggccgaggtc gcgctcttca acgacacctg
    13681 gaacgagtac cagcggccgg aggtcgggga gggagcggtg aggctcttcg ccgccgccgg
    13741 ggcgcgggtc catctgccgg aggtggtgtg ctgcgggcgg cccatgctct cggagggact
    13801 cgtggacgag gcgcgggaga acgcgcggcg caacctggag atactggccc caatgctgga
    13861 gtggggcatc ccgctcgtcg gtctggagcc gagctgcatc ctgaccatgc gcgacgacta
    13921 ccgcaagctc ttgccggacg atccccgcgt cgagaagctc gaagcggcca cccggctctt
    13981 cgaggaggcg ctgctggagc tggtagacga cggcgcggag ttgccgctag aggagggcgc
    14041 gccagtcctg ctgcacggtc actgccacca gaaggcgctc gtcggcacgg gaccgacgga
    14101 gcggattctc gggctcgccg ccgacgtgga ggtggtggac tcgggttgct gcgggatggc
    14161 tgggctcttc ggttacgaga aggggcacta cgaggtctct atgaaaatgg gtgagcggcg
    14221 cctattcccg gcggtcagga gcgcggagga gcgtaccgtc gtcgcgccgg gaacctcctg
    14281 tcgggagcag atcctgggcg gcaccggacg ccaggcccta cacccggccg agtacctggc
    14341 gagctgcctc gaagaccgtg cctgaacgcg gctccgagga ggcgctctgg ttcgccttcc
    14401 gggacgagca gttgctcgtg ctggacggag acccggtccg cgtcccggat tcgagcacgt
    14461 tcgaggagct gggtctcgaa gctgcgttcc ggtgggagat cgggacgctg aacggggcgc
    14521 gctgctgggc ggttgagatc gactcggacg gcgggacgcc tgatgggatg gtcttgcggg
    14581 acctgcgcag cctgttcgcc gggatagacg agggcttctt caagatggcg gggaaggcca
    14641 agcaggtcgt cggctggcac acgacgcacc gcttctgcgg gcgcgacggt acggagaccg
    14701 ggccggtggc gggcgagatg gcgaagcggt gcccgcggtg cggcatgatc tactacccgc
    14761 gcctgagccc ggcgatcatc gtgctcatcc gcaacggaga ccacgtccta ctggcgcgct
    14821 cgccggggtt cccgccgggc atatatagcg tcctggcggg cttcgtggag ccgggcgagg
    14881 cgatagagga ggcgataaag cgggaggtcc gggaggaggc cgggatcgag gtggagaaca
    14941 tccggtactt cgggagccag ccgtggccgt tcccgaactc gctcatgatc ggctttaccg
    15001 ccgagtacgc ggggggcgag cttcgcatag accctaccga ggtcgaggac gccggctggt
    15061 acaccccgga caggatgccg cccctaccgc cgcgcttcag catcgcccgc tccatgatcg
    15121 aagacttcct ggaccggcac ggtagcaatc ccgagggccg atagagcaga tattcaggcc
    15181 caggcgtaat acccgcgaga taccggtccg ctatcatgta atgggcgtaa tacggtgggt
    15241 gtgccgcacc agggtcgagg ttgcttatga gacggttggc cggaccaggg agagtgggtg
    15301 cgttcaggct cgccgctctc gtggtcctgg cggccgcgct ctccgctttc gccgcttacc
    15361 tggtctgggg agataccggt cccttctccg gctccctggt ccgggtgtcg aacgatgcgc
    15421 cgggacatta ccgggtcggc cgtttcctgg tggatgttaa aggagagggc aacgaacctt
    15481 ccggcgtggc gttgagcatt gcgcacggct cccaacccca ccgcgtcctg tggagcagca
    15541 ttcccggaga gagcttcgtc tcggcggcga aaggagaaga gtcggtcagc cagtccaggg
    15601 cgcacttcca cgtcgaggac gagatctcgt ccctgcacga agaccagacg atagactcga
    15661 taaagaagga cggcgatagg ctggtcatcg aggggcggct cctcgacgga ggcgcggatg
    15721 gtgtgagata cgagcttgcc ttctcgcagg ctagcgacgg gcggctgcgc ttccaggtaa
    15781 aggtggatga gccgtcgtat gatcgagtat tcctgaccta cgctacgtcg cccgaggagc
    15841 gcttcttcgg gttcggggtt cagtacacgt acttcgacat gaaggggcgt gaggtcccga
    15901 tcttcatcca ggagcagggg atagggcggg gagcgcagcc gatcacactc ggcgccgact
    15961 ggcaggccgg tgccggcggc acacccttca ccagctacgc cagcgtgccg cactacatca
    16021 cgagcgagat gcgctcgctg ttcctcgaaa actacgagta ctcggacttc gatctacgcg
    16081 acggcgaccg ggtgcagata gaagtattct cgccgcagat gaccggccag ctcctcagcg
    16141 gagacacccc gaaggagctg atcgaacagt acaccgaata ctccggccgg atgcgtccac
    16201 tacccggctg gatcctctcc ggcgcggtca tcggcttgca aggcggcacc gacacggcgc
    16261 gcaagcggta cgcggagctg aaagccctcg acacgcccgt cgccgccctc tggctccagg
    16321 actgggtggg ccagagagag accagcttcg gtacccagct ctggtggaac tgggaactgg
    16381 ataaagacca ctaccccggc tggagtaagc tcgtctccga ccttaagcgc gacgacgtga
    16441 gggtgatgac ctacgtaagc ccattcctgg ccaatgtcgc tgacaagccc cactaccgcc
    16501 gcgacctctt cgaggaggcc cggaggaaag ggtatctcgt cgagaacgcg aaaggcgagc
    16561 cgtacatgat cgagatcagc gacttctcag ccgcgctggt cgactttacc aaccctaaag
    16621 cgcgggcctg gtatgaggac atcatcgaga agaacctgat agacgagggc gtgtcgggct
    16681 ggatggcgga cttcggagag ggactaccct acgacgccgc gctcgattcc ggggcggacc
    16741 cgaagagcta ccacaatcgc tacgccgagg agtgggcgaa ggtaaaccgt gaggcaatcg
    16801 agagagcggg acacgaggac gacattgtct tcttcaaccg ctccgggtac acgaggagtc
    16861 cggagtacgc gaccctgttc tggctcgggg accagctcgt aagctgggac aaatacgacg
    16921 gcatcaagag cgccgtcacg ggcctgctct ccagcggtct ctccggctac agcctggagc
    16981 acaccgacat cggcgggtac acggctatcg acagcccctt gaaggattac caccgctcca
    17041 aagagttgct catgcgctgg acggagatgg ctgcattcac ggccgtcttc cgcacccacg
    17101 agggcaacaa gcccgaggac aattaccagt tctactcgga cagggagacc ctcgaacact
    17161 tcagccgctt cgcgaaggtc tacgcggcgt ggaagccgta cagggagaag ctggtaaggg
    17221 aggccgccgc gtccgggatg cccgtagtac ggcatccgtt cctggagtac ccggaagatc
    17281 ccgaggtcta cggtctcgaa taccagttca tggtagggtc ggagttcatg gtggcgccgg
    17341 tcctggaccc cggcgaggag acggtcgagg tatatctgcc ggccggtcgc tgggtacatc
    17401 tgtggaccgg gaagaggtat ggctctcgcg gtaagggtgt ccacgtgacg gtggatgcgc
    17461 ccatcggaga gccggccgtc ttcttcaagg aaggttcgag cgcgggacgg gacttcaggg
    17521 aagagctgag gaggcaggag ctactacggt gaggttcgtg cggtggcggt aacgcgatgc
    17581 gtgggttacg cggtccgcta gccgacgggc gtctaccatg tgggcgaccg ctcagagagg
    17641 atcggacggg ttggggatca gcttccccgg gttcatcgtc gcgtcgggat ccacggccct
    17701 cgccagggct tcgagcatgg cgactccccg tgcgcccttc tctttctcca ggtacggtag
    17761 atggtcggcg ccgacgccgt gctggtggct tatggtcgcg ccccgcgcga cgatggcgcg
    17821 cgaggccgcg gcctttaggg cccgccagcg ttccagagtg gattcggggt cggccgccag
    17881 ccagaagaag aacgtgaagt agaggctcgc tccggtgggg tagacgtgcg agaggtgcgc
    17941 catcgccagt acgcgctcgc cgtcgagcgt ggagcgcagc gcccgcagga catcgcgggc
    18001 ggtcgggacc acggcgctcc agacggtcgc cgtctccagg gtatccaccg cccagccctt
    18061 ctcccacagg gtgttgcgga ggtacgggac gcggaagcgg ttgttccgcc actccctgcc
    18121 ggccacgtcg ccgatagcca cgcccttgcg ggccttcgat atctctgccg ccctcgcgcg
    18181 ggctccgcgc acggtccggg aatcgcccgt ggcgcccagg atcaggagcg acttctcctc
    18241 ccgcaggccg cggcgggcca ggagaccttc gaggagccgc cggacgtagg ggttcaaagc
    18301 ctggtgaagc agggagttgg tctcctcggc gtcgctcagg cgcagcatgc tcagggggcg
    18361 tggatcctgg gccatgatcc gcacggcctc gatgccgtcc tcccaacccg ggaagacgat
    18421 gccccagaaa cgttcctctt ccggcagggg gtgcacgcgg acctgtactt cggtgatcag
    18481 gccgagcctg ccctcggaac ccaggacgac gtgccgcagg tccgggccgg ccgccgaggc
    18541 cgggaagggt ggcaggtcca taggtccttc cggggtgagc agccgtccgc cgcggaagag
    18601 gtcctcgatg cgcccgtagt gcagggactg ctggccgctg gaccgggtgg cgacccagcc
    18661 tccgagcgtg gagtactcga aggactgggg gaagtgtccc agcgtgtagc cccagcccct
    18721 gagctgggcc tccagccagg gtccgcgcac accggactcg aaggtggcga gcatgctctc
    18781 ctcatccaga tctctgagcc ggcccatccg ctccaggctc acggtcaggg tggggtgttc
    18841 gtcgagtccg gggtttactc cgcccaccac gctcgtgccc cctccccacg ggatcacccg
    18901 cgctccgatt cgccgggcgt gggagaggag atccaggacc tccgtctcct ccgtgggaaa
    18961 tgcgaccccg tcggggaaag cctcgatcct cccgctcctg agcgccagca ggtccgggaa
    19021 gctctccccg cgactgtaac gaaccctggt ctccgggact ctctcgacga gaggatggtc
    19081 cggcaggcgg gaggggggcg gtgagatctc ggcgaggcaa acgtcttcct gcggcacgcc
    19141 ggggccgatg cgtctctcca ggtatctctc cgccggagac gacagcgcgt accgcatgga
    19201 ctcttcaccc cacccattcc agcgccgcat ggtgctcgaa tgataccgct ctctacgcgg
    19261 cggcccgaga gacgaggtta cgaccctgcg ggctccggac tgtaatgagt ctcccagagc
    19321 tttaaatagc gccgccactc tgcttcccag cgtcccggat cccagccggg gaggtctttc
    19381 aggcgggctt ccatctcgcc cccgagcgtc cttcctcctc cggggagcag atgtcccagg
    19441 cgtaccctcc gcagcaggag gtcctctagg tggaccacgg cttctctctc gaacgcccac
    19501 ctgagctccg cccaggtgta tggcgtgtcg gcaatctgct gaaggtcgtc ttctctcatc
    19561 cactcaagaa aagcctccag gtcgggcccg agccgcccgg cgagtctgcg ccgcaggagg
    19621 tgcggcagcg gcgagagggt ctcgttggct ctctcgatga tctttctctg gagaggttcg
    19681 aggccaccgg aggcgcttcc gttcgaccgt cttccgaggt ctgctgccac ggtcctcacg
    19741 gcttcacggg ccatcgggtg aaaggtggtg agcttgccgc cggcgattgc caggtagttc
    19801 ttgtcgcgcc agatggcgtg ttcgcggggc tcctgcgacg ggtggtctct gccgccgaag
    19861 atcaccggcc tcacacccgc gaaggtgccg agcaggtcct ctggggaaac gtggatgtcc
    19921 ttgaacctgt gccggacggc ttcgaggaga tagtcgctct cttctctcgt cgcggccggt
    19981 tccaaccgga gcggctggtc gtggtccagg tccgtcgtgc ccacgagcgt caccccctcc
    20041 cacggatacg agaagaccgg acgcccgtca cgcgggtgcg cgaatgttat cgcctgggcc
    20101 acgggtagct tctcccgcgg caggacgaga tggctccccc gaaggggacg aagcctcggc
    20161 gcaccgccac cggctacgat ctcggcggac catacgcccg tcgcgttcac cacgaatctg
    20221 gtcttcacct ccgtgacgct gccggtatcc gcgtcgctga ccgcgaccgc ccgcacgttc
    20281 ccttccctgt cccggagtag ctcatcggcc cgcgcgtagt tcaacgcccg cgccccgaag
    20341 ctcatcgcct cgcgcaacac cctgaagacc agccgcgcgt cgtcggtctg ggcgtcgagg
    20401 tacgcgaagc cccggcgtaa tccgacgaga tccaggttag gcgccgacag ccagacgtcg
    20461 agcgcgtcta ggccgtggtg attctttccg cccttcttca gaaggtcgta gacggagagg
    20521 ccgaaatcca gcatccacgg tcccggccgg tctccctggt agaccacgta gaggaaccgc
    20581 agcggctcca ccagcccctt tcccgttcgc agcaggctct cccgctcgcg gaccgcttcg
    20641 cgggtgagct gtatctggcg ctcgcgcagg tagcgcagcc cgccgtggac catcttcgag
    20701 ctgcggctgg aggtccccca ggcgaagtct ctctgctcca cgaggaggac ggagagaccg
    20761 agcctcgccg tctccttcgc caccccggcc ccggtgatgc cgcccccgat caccaccacg
    20821 tcccacacac ggctcgcagc ttcgtcccac gtcgaacccc ggtaaccttc gccaaaatcg
    20881 ggcatggtaa cgaagagtat aagccatgaa atcttccggg tgttcccgcc aggttccccg
    20941 gcggggcggc gcttccagga cgagttgaag agcggcggac tgctgtagaa aggtgtatcg
    21001 tggggcgatt gtcccaggct caagcagtat aaaagtctga tgacaggaga gaaggtttac
    21061 aggatcatgg gttccctagc aggctggcgt cccgtgaatc cctcctcccc ggacgccggc
    21121 ctcatttgtg aacggcctgg aggtggggag ttgcgggtgg aggtctcagg ttggacgtag
    21181 agtgtgtaat ccgggtggct atagtcgatg atcagcggct cttcgcgacc gggctcgcca
    21241 ggatcatcgg cgcgcagccg gatatggagg tggtggggga ggcgcatacg ggtgaagagg
    21301 gcgtgtccct gtgcgcggag gtggagcccg acgtggtact gatggacctc tcgatgccgg
    21361 tgatgaacgg ggtgagcgcg acgcgcaaga tccgcaccat cctgccgagg accagggtgc
    21421 tcatccttac ggtgcacacg gacgacgcgc acgtctttca ggggatcaag gccggcgctc
    21481 agggatacat cctgaaggac tgcacccccg aggacctgac gcgcgcgatc cgcacggttt
    21541 acgccgggga tacgatcatg gctcaagaca tcgcccggaa gatgctcact accttcgagg
    21601 gcatccgggc gaacaacgcg gacatcgcgc ctcacctgac ggagcgcgag ctcgacgtgg
    21661 taaaggcgct cgccaggggt atgagcaaca aggagatcgc gcagaccatg aatatctccg
    21721 agaagaccgt ccggaaccac gcctcgaata tttacaagaa gctccacatc ttcgaccgca
    21781 cccaggcagt cctctacgct atccgcaagg gactggtgga catcgaggac ctggagccgg
    21841 gctagcgcta tcggctagcc cgcggggtta gacgtctgcg ctctccggga tggggatctc
    21901 tacgcggatc ccgactcccc tgcccggatg gccttcgatc tccagctccc cgcccagcgc
    21961 ggaggcccgt tcgcgcatgc cggatatgcc cagcccgatc cgggcggagt gaggatcgaa
    22021 acctctcccg tcgtccctga tctcggcccg caagaggtcc ccttccatgc tcaacgcgac
    22081 ttcgacccgg ctggcgccgg agtggcggcg ggcgttcgag agggcttctt gcaggaccct
    22141 tagcatctcc acgctgacgc actccggcaa ctcctgcggg aacccgtcct ggagcgtgag
    22201 ggttatctcg cgttcggggg tcatctggga gttcagctcc accagcgact cgaccgccct
    22261 cacgaatgtc tgacgcccct ccaggcgcag gttgtagata gcgtttctaa ggcccttgct
    22321 cgctctccgg agggcgtcta tctcctgttc gagcccgatc tccgcgcccg cgcgccgcgc
    22381 ctctatccgg gcggcctgca tcccctggag cgcgcccgcg aggtcctgaa gtacgacgtc
    22441 gtgcaggtcg cgggcgatcc ggcgccgctc cgcctcccgg atctcgtaca ggctctcctc
    22501 cagcctcttg cgctcggtga tgtccgtgga gacgccgacg atccctatgg cgttcccctg
    22561 atcgtcgtgg accagggagt ccgtcacgta ggccgggaac gacgagccat ccttgcggcg
    22621 ggtcacgaac tcgccttccc acgacttgcc agcccggagt tggatcatga tctcctcggc
    22681 aacccggatc tcctttggac caatggtgag ctctgagatg ttgcggccga cagcctcctc
    22741 gcgcgaccat ccgtagagct tctccgcata ctcgttccag tggatgacct caccctgcat
    22801 gtcggtggcg atgacggcgg tctgcacttg ctccagaagg tgggcctgga accgtacctt
    22861 ctcttcggcg cgcttgcggt cggttatgtc caccagcacg ttcaccgccc caagcagctt
    22921 ccccgactcg tcgtggatcg ggttggcgtg cgccagcgcc gttagccgct ccccatccgg
    22981 cctctccacc acgatctccc gcccgttgta ctccctgtcc gttcttagcg ccagagccat
    23041 ccagcactga tcgtgggaga tgggaggacc gtccgtggag tagagcttga aggagccgca
    23101 gtagcggtca actgggtcgt ttagctttgg ggcgcggccc cagagcttta cggcgtgctg
    23161 gttgaagtag gtgattaggc cgtcgggatc gcaggtgtag gctccggcgg gcagtttctc
    23221 cagcaaccgc cggaactgca gttcgctctt tcgccacgtg gacccggccc gccatccccg
    23281 acccagccct atctccagtg tctcttcccg catgtgcttc tttctttaga acccggatag
    23341 taaagggttg ccgcctgatc ggttgccgac tgagccaacc gcccggtgcc tcgtcacttg
    23401 atgataaccc accgggccgc cgtagggtag ggacgaattg ctctttgttc tcccgtcctg
    23461 tccatgcctt atcgtcttaa actgtcttgt gtcacacgga tgtcgcgcgg gatgtttcgg
    23521 acccggctgg gtaatttgac ctatatgctc cctcaacatg taccggttta cctgtgcagg
    23581 ggatacagta tgttgtgaac ggggctgagt cccagggttg actggcagtc cgactgtggg
    23641 gaaaggggtt tgccagcccg tcaggaggaa gatatgcgat gcgggtaatc gagcgcgaaa
    23701 aagaacacta tgaagtccag gaagttcctt acgggaaagt ctatagctgg cgtcccgaac
    23761 gcatactctt tgagtgtgat tgcggagaga ccctggcatg gatggtgccc gttaccgtct
    23821 gccggtgcgg cgcgaagcat actggcatag ccttgaagga gggaccgccg ccggacgaac
    23881 acacgcggca cccgtggctg gcggagtacg aggaatggcg gaaggtgaag ctagccaacg
    23941 acctgcggca cgaatactac ggcttcttaa aggcggagga cgaatgatca gaggggtaga
    24001 gacgaagaca gagcaggcgc cctccatcgg agagcactcg gtggccgtgt ggtacctgat
    24061 ctcgcgctgc gagcataacc ggctggatat cttgacggta tttcctcgcg acgacgagga
    24121 gacagtgccg gtcttcggca cgcggcgcat ggccgaggag ttcctgcggg atggcggctt
    24181 cgagcaaggc tggcgggtca gggagtcaac ggcgggagag ttgatctcgc tgctcctgag
    24241 ttgtctggtg gacttgaata gcgtctccat ggacccctct cccgatctcg cctttaccgg
    24301 gaccgcgccg tcgaagcgcg cgaccaggag agagtttata gccgccctca tgggcagacc
    24361 gctcctggtc cccgtcccct gaaaaacttg aacgactgta tctcgcgaga ccgggagggc
    24421 taagcgatct cggaggcgcg gcccgcggcc tcttcgagcg cctgaagggc tgattcctgg
    24481 tccattccgg tgctccgcag gaggtccacg gcggcggacc ggatctggcc gacgagcacg
    24541 ctggtcgcga ggtcgtgccg gtcgctgagg atctccgtcg ccttctgggc cgcgccgagg
    24601 gcgtaacgtc gcgcctcttc gtagccgccg gactcttcga ggaaggtctc cagagccttc
    24661 acggcgcggg agaggtccag cacagcctcc gggagccggg gaggagcggc gcctccgcgg
    24721 cgcgtggcgt tggccgcacc acgcgccagg acgcgcgcgt ttataacggc caggtctatg
    24781 cggaggcccg cgtttgcgta gagctccaga tgctgcaatg cgcggcgtcg ggtgggagag
    24841 agccgggcgg tctcgtgtcc ggcggcgaga gcctcgttga agcggcgaac ccgcacatcc
    24901 aactcccgcg cccgcaatag cgcgcgcctg gcgcggtccg cgtcgccgcc cgcgagagcg
    24961 gcggctactt cttctaatac cgtgacgagt tcctcgaata ccggggccgc cgccctctcc
    25021 accagccgct ccgggttggc ggggaacagg tagtgaatgg cgagggccac gctcccgccg
    25081 atgagcgcgt cgaagaagcg cgtgggggag aagaaggagt ccggcggctg caagatcacg
    25141 acgagcaggg cggagatggc cgcctggttt accagcagcg tgcctccccc gaagaagacc
    25201 gccgcaccca tcgccagcgc gaccaccaga ccgatctgga gcgtcccggt ccctatgagg
    25261 aggacgatga agtctgccac cagcagaccc accgccaccc cgaatacgat ctcgaccgcc
    25321 cgccgtcccc gctgtccgag cgttacgctc agagagatca ccgccgctat tgacgcgaag
    25381 aatggacgct cgtgccccag gacccacacc gccacgaagt acgccgcgct cgcagcgacg
    25441 gcgctctgcg ccaacgacca gatgttcgcc ctgagccgct ctttgcccct ccatagaccc
    25501 cgccgaaacc tcgaaagcaa acgtacttct cccggagaac ccgccgccag agacggccca
    25561 actatacacc cttggtgcga gcatccgtac cgctctcgcg tcgtttccgc ggtggtgcga
    25621 cactactacg ccccctgcgg tacccggcgc cccaccccat cgagcacgtg gaacggcact
    25681 ctactgatac tgtcctgagc gccggccttc attgccccgc ggtgatgacc ttgctacact
    25741 ggaaatggac atatgtccat ttgaatagtg tcggactatt gtccgcttgg cgggagagga
    25801 ggttgagata tggctgtaga aggtgggagg cgccgggagc gcagggatgc ggtggagagc
    25861 cggcggaagg tattgggcgc ggcgcggcgg ctcttcgagg agcggggcgt ggacgcggtt
    25921 agcatgcacg agatcgggcg cgcggcgggg gtcgggcagg ggacgctgta ccggcgtttc
    25981 gagcacaagg gcgcgctgtg ttcggcgttg ctccaggagg agatcgaggg tttcgaggaa
    26041 gaggcgcggc ggagggttgg gagtcgaggt tcggcgctcg gacgcctgaa gtggtttctg
    26101 gggcgtctgg cccggttcaa cgacgagaac gcggcgctcc tcggggcggt gagggacgcg
    26161 gcgggcggcg aacggcgcat agagacgcac cgaaacccct tctatgaggg actgcgggag
    26221 aacgtggcgg agttgctgtg ggaggcgttg gaggagggtg agatcccgga agagttggac
    26281 atagagtgca tgacggatac cctactcgcg gccctcggca tagacctcta cctctatcag
    26341 cggcgggagc tgaagatgag ccgggagcgg atcgtagact cgttgcgttc tctgctggag
    26401 gctcttcgcg ttcgcggttg acgagcgctt acagtgaacg cgctcgtggt agggggtgca
    26461 cgccgttcat ataatcggcg aggagccaga gatgaggagg ttgccagcat gggtcgacaa
    26521 cgagtgaagc ctggtcccgg acaggagtcg gtgtgggact acccgcgtcc gccgcggatg
    26581 gaggacgtgg acaagaaggt gaaggtcgtg ttcggcgggg tgaccatcgc atacacgacg
    26641 cgcgcgaaga gggtgctgga gacaagccat ccgccggtct actacatccc gccggaggac
    26701 atcaggatgg agcacctggt gcctgcggat gggagctcga tgtgcgagtg gaagggccgg
    26761 gcgcgttact acgacgtggt gacggaggat cggacggagc gccgggcggc gtggtactac
    26821 ccggagccgg tttggtcatt ctcggggctc aagggctatg tggcgttcta tccatcgcgg
    26881 atggacgggt gctgggtcgg gggagagaaa gtcgaggcgc aagagggtga cttctacggc
    26941 ggttggatct cgccggagat agtcggtccg ttcaagggcg ctccgggcac ctggggttgg
    27001 tagggccgtt cgattggatc ctgtcgcccg ccttcgccgc gaatacactc gcacgggcct
    27061 ccgcgaatcc gacgtagacg aggacccgat agagcagttc cgccggtggt tcgaggaggt
    27121 tatggaagca aatctgcacg agccgaacgc catgaccgtc gccacctcca ccgccgacgg
    27181 taaaccatcc gccaggatcg tgctgctaaa aggcttcgat gagcggggct tcgtcttcta
    27241 cactaactac gagggccgaa agagccggga gatcgagacg aacccccgcg ccgcgctcct
    27301 gttctactgg ggagagctgg agcggcaggt caggatcgag ggccgcgtag aacggatcgc
    27361 ggaaacagac tccgacgcct acttcgccag tcgtccgagg gggagccagc tcggtgcgtg
    27421 ggcctcggag cagagccgcc cggtggagga tcggggcgct ctcgaagaga ggctggacga
    27481 ggtcgaggag cggttcgggg agggcgacat cccgcgtcca ccgttctggg gcggctaccg
    27541 ggtcgagccg gagagtatag agttctggca gggacgcgag agccggctgc acgacaggat
    27601 tttgtaccgg cgcagcggca gtgggtggaa gatcgagcgt ttgcaacctt aggactgccg
    27661 ggatggatcg ccggagcaga aagcccgata ttgctgctaa gatgcttctc gataggtctt
    27721 atctatgcga gaggagtctt cttgaagctg gttacgtact cgacggatga cggggtgccg
    27781 agggtaggag cgctggaggg agaggagatc cgggcgctcg gaggagcggg gtcggacatg
    27841 atcgagttca ttgagcgcgg cgacagcccg gagccgggcg gcgagaccgt ggcgctctcg
    27901 tcggcgcggc tgcacgcccc ggtgctcacg ccgcagaagg tcattggtat cggccttaac
    27961 tacgaggatc acgccgccga gaccggcgcg gacataccgg agaagcccat cgtcttcgcc
    28021 aagtatccca acagcatcat cggaccgggc gaggagatcg tgatacccca gatcaccgag
    28081 caggccgact acgaggcgga actggccgtg gtcatcggcc gcgccgcgcg caacgtctcc
    28141 gagtccgagg cgctggacta cgtcttcggc tatacgaact gcaacgacgt ctcgtccagg
    28201 gaccttcagt tctccgaggg cgggcagtgg acgcgaagca agtccataga caccttttgc
    28261 ccgataggcc cgtacatcgc tacccgcgat gagatagaag accctcagga cctcgcg
//
LOCUS       DASSMB010000176         6949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1309905, whole genome shotgun sequence.
ACCESSION   DASSMB010000176 DASSMB010000000
VERSION     DASSMB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309905"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VFJ72_10440"
     CDS             <1..261
                     /locus_tag="VFJ72_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479917.1"
                     /translation="ELSISKAHYMAERLQASGLRLRYPHAPFLWEFAVEMPDVKLVNE
                     ALLESGIVGGLDLGDGAMLVAVTEKRTRDELDAFVEVVANAG"
     gene            251..1666
                     /gene="gcvPB"
                     /locus_tag="VFJ72_10445"
     CDS             251..1666
                     /gene="gcvPB"
                     /locus_tag="VFJ72_10445"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET7479918.1"
                     /translation="MLGDLIRDKGREGRQGYTLPKPGVETKKLEDILPKKALREEKPK
                     LAEVDEPTVVRHYTNLSRKNQGIDTTFYPLGSCTMKHNPRANEAAAAVPGFSGLHPLQ
                     PEAQAQGALRVMHELQGFLAEISGLPAVSLQPLAGAQGELTSILMVKRFFEDAGDDAR
                     TTVLVPSTAHGTNPATASMAGFKSKEIGLDEHGGVDVNELRSMVDESTAALMITNPNT
                     CGVYERNIAEIAGILHEAGAFLYMDGANMNANMGRMRPAEAGVDLMHFNLHKTFSTPH
                     GGGGPGSGAIACVEKLAPFRPDPTVEEAGESYAFVRPDKSIGRVAGFHGNFGQLLRAW
                     TYIKMHGPELKDVTDMAVLNANYVRAKLRGTYRIPYDELCKHEVVVQPPEGMKALDIA
                     KGLIDHGYHPPTIYFPLVVKEAMLIEPTETESKETIDDFIGAMLELAEAKPEDLQEAP
                     THAPTRRLDETRAARNLKARW"
     gene            1669..2748
                     /gene="gcvT"
                     /locus_tag="VFJ72_10450"
     CDS             1669..2748
                     /gene="gcvT"
                     /locus_tag="VFJ72_10450"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET7479919.1"
                     /translation="MPELRRTPLYEEHKQLGARLVDFAGWEMPVQYAGIKAEHEAVRT
                     HAGLFDVSHMGEVVFRGPDSEAAVQRLVTRDVARLAEGQAGYAAVCYEDGGTVDDVLV
                     YRRPEDFLIVVNASNREKDVAHFRENTADLDVEVADESDDWALLALQGPEAVGLLGNF
                     TDTDLAGIKYYRFVEGKVDGATAILSRTGYTGEDGFELYLRPDDAPRVWKRLIEAGAS
                     PVGLGARDTLRLEAGMCLYGNELDAETTPLEAGISFAVHLTKEPQFIGQGALLREDAE
                     GLKKKLVGFEMEGRGIARHGHTVTRGAEIVGAVTSGTQSPTLGRAIGLALVEPEVEDR
                     FMVLIREKPVPAKTVPLPFYKREKR"
     gene            2765..3142
                     /gene="gcvH"
                     /locus_tag="VFJ72_10455"
     CDS             2765..3142
                     /gene="gcvH"
                     /locus_tag="VFJ72_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET7479920.1"
                     /translation="MSVPGDLQYTKSHEWVRTDGDTATIGITEHAQDELGDVVFVELP
                     EEGATFDAGDSFGTVESVKAVSDLYAPVGGEVVEVNTALEESPEKINEDPYGDGWILK
                     LRTSGQGDLLSADEYQKVLDEES"
     gene            complement(3580..4215)
                     /locus_tag="VFJ72_10460"
     CDS             complement(3580..4215)
                     /locus_tag="VFJ72_10460"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET7479921.1"
                     /translation="MRATLQPGLTNRLTKYLRTTQQLIEEGRDAVSSKELGDFTGINP
                     VQVRRDLNAIGFSGTRGVGYQAYDLVEAVRSILGLRQTYNIALVGAGNLGSAIAASTI
                     LPKRGFVIHDVFDNDPKKAGRTVGNVVVKHLDELKKSVAEAEEIIGIIATPASSAQEV
                     AELMVDAGIRVILNYTDVLLHVPYNVDVHRIDPTAQLMHTLYYLTQVGEAS"
     gene            4459..5229
                     /locus_tag="VFJ72_10465"
     CDS             4459..5229
                     /locus_tag="VFJ72_10465"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HET7479922.1"
                     /translation="MATILWGALVILVTVGVTVAGMILARRRVPLPLREAHNNTTATI
                     FGALYVMYALMVGFSAYFVAYQFDTAQKNVESEAGSVEEIYYLAEQFPEPDRRKVQGL
                     TESYAKTVIQQGWPMMKQGKLSPRAGETADELRRTVLSFEPKTSSQQAVYAQALLVLQ
                     DLDEYRALRLLDARTGIPLILWVVLVVGGILTVAFTYLFGMKDHRLHLLMVVAFTVVL
                     ILTLYTIRALEYPFDGIVQIGSDAFETVLDNIRAGGGQ"
     gene            5508..6482
                     /locus_tag="VFJ72_10470"
     CDS             5508..6482
                     /locus_tag="VFJ72_10470"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7479923.1"
                     /translation="MLAFSTRDDTDTEETGFREALRAASREDGGASIRPMSDVDLDRI
                     LELRTVVNWAADPRAFDLLRGIRDARWAVAETPDGSLAGMVGAVPFGGIGILCHLAVH
                     DDYRNLGLGARLSAWAILYLRSRGASTVRLYSTRRAEKLYRSMGFRPAVQRTIYRLDE
                     ASRNPLARQEADGHHVGTMLFGDLPEIYGVDHWTYGADRSALIFATLRLHPGRGLVAR
                     DSAGRVKGYLIRGEAGHATGHATRIGPFVASEPGVARLLLQNALDGAEGAAIEVTVPG
                     PGNGPAHALLKEFGFTGRRDRLRMDLGEKLCSCRSALTHYGTTPYLAT"
     gene            complement(6475..>6949)
                     /locus_tag="VFJ72_10475"
     CDS             complement(6475..>6949)
                     /locus_tag="VFJ72_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7479924.1"
                     /translation="VLVPVSGGGLISGVAAALKLNRPDINAIGVEPELAADARASLRS
                     GRIVEFSAEEVSRTVADGLRVRRLGNAPFEHVRAFVDDIIAVTEEEILDAMRRLALRV
                     RMVAEPSGAVSFAAYLFHREELPPTRRNVAVISGGNVDPDLLAGVLASGGSVRSM"
BASE COUNT         1280 a         2141 c         2276 g         1252 t
ORIGIN      
        1 gaactctcga tctcgaaggc gcactacatg gcggagaggc ttcaggcttc gggtctcagg
       61 cttcgctacc cgcacgcgcc gttcctgtgg gagttcgccg tcgagatgcc ggacgtgaaa
      121 ctggtaaacg aggcgttgct ggagagcgga atagtcgggg gtttggacct cggagacgga
      181 gcgatgctcg tggcggtgac ggagaaacgt acaagagacg aactcgacgc cttcgtggag
      241 gtggttgcga atgctgggtg acctgatccg agacaaaggc cgcgagggcc gccagggcta
      301 cacgctcccg aaacccggcg tggagacgaa gaagctcgaa gatatcctgc cgaagaaggc
      361 gctccgcgag gagaaaccga agctggcgga ggtggacgag cccaccgtcg tccgtcacta
      421 tacgaacctc tcccgcaaga accagggcat agacaccacc ttctacccac tcggctcctg
      481 cacgatgaag cacaacccgc gcgccaacga ggcggctgcc gcggttccgg gcttctccgg
      541 gctgcatccg ctccagccag aggcccaggc ccagggcgcg ctgcgggtca tgcacgagct
      601 acagggcttc ctcgcggaga tctccgggct gccggcggtc tcgttgcagc cgctcgccgg
      661 cgcgcagggc gagcttacga gcatcctgat ggtcaagcga tttttcgagg acgccgggga
      721 tgatgcccgc acgacggtcc tcgtgccttc cacggcgcac gggaccaatc ccgcgaccgc
      781 ttcgatggcc gggttcaaga gcaaggagat cggtctcgac gagcacggcg gcgtagacgt
      841 gaacgaactg cgttcgatgg tggacgagag tacggccgcg ctcatgatca cgaaccccaa
      901 cacctgcggc gtctatgaac ggaacatcgc cgagatcgcc ggcatcctcc acgaggccgg
      961 cgcatttctg tacatggacg gcgcgaacat gaacgccaac atgggccgca tgcgtcccgc
     1021 cgaggccggc gtggacctca tgcacttcaa cctccacaag actttctcca ccccgcacgg
     1081 tggcggcggt cccggttctg gcgccatcgc ctgcgtcgag aagctcgccc ctttccgccc
     1141 agatcctacc gtcgaggaag caggcgagag ctacgctttc gtccgccccg ataagagcat
     1201 tgggcgcgtg gccggttttc acggcaactt cgggcaactc ctccgggcgt ggacctacat
     1261 caagatgcac ggcccggagc tgaaagacgt gaccgacatg gccgtcctga acgccaacta
     1321 cgtgcgggcg aagctccggg gtacctaccg catcccctac gatgagctgt gcaagcacga
     1381 agtcgtcgtg cagccgcccg aagggatgaa agccctcgat atcgccaagg ggctcataga
     1441 ccacgggtat catccgccca cgatctactt ccccctcgtc gtcaaggagg ccatgctcat
     1501 agagccgacc gagacggagt cgaaggagac catcgacgac tttatcgggg cgatgctgga
     1561 gctggccgag gcgaagcccg aagacctcca ggaagctcca acccacgctc ccacgcgccg
     1621 cctcgacgag acccgcgccg cccgcaacct gaaggcgcgg tggtagcggt gccggaactc
     1681 agacgcaccc cgctctacga agagcacaag caactcggag cccggctggt ggatttcgcc
     1741 ggatgggaga tgccggtgca gtacgcgggg ataaaagccg agcacgaggc cgtccgcaca
     1801 cacgccggcc tcttcgacgt ctcgcacatg ggcgaggtcg tctttcgcgg cccggactca
     1861 gaggcagccg tgcagcggct cgtcacccgc gacgtagctc gtcttgccga gggtcaggcc
     1921 ggctatgcgg cggtgtgcta cgaggacggc gggacggtgg atgacgtgct ggtctaccgc
     1981 cgcccggagg acttccttat cgtggtaaac gcctcgaacc gggagaagga cgtggcccac
     2041 ttccgggaga atacagccga tcttgacgtg gaggtcgccg acgagtccga cgactgggct
     2101 ttactggcgc ttcaggggcc ggaggccgtc gggttgctgg ggaacttcac ggatacggac
     2161 ctcgcaggga tcaaatacta tcgcttcgtc gaaggaaagg tggacggcgc cacggcgatc
     2221 ctatcccgca ccggctacac gggggaagac ggcttcgagt tgtatctgag gccggacgat
     2281 gcgccccgtg tctggaagcg ccttatcgag gctggagcat cgccggtagg gctcggagcg
     2341 cgggatacgc tgcggctcga agccgggatg tgcctctacg gcaacgagct ggatgcggag
     2401 acgacgcctc tggaagcggg gatctccttt gcggtgcatc tgacgaagga gcctcagttc
     2461 atcgggcagg gagcgctact tcgggaggat gcggaaggac tcaagaaaaa gctcgtcggc
     2521 ttcgagatgg aggggcgcgg gatcgcgcgc cacggtcaca ccgtaacgag gggggccgag
     2581 atcgtgggtg cggtaaccag cggtacccag tcgccgacgc tcggtcgggc catcggactt
     2641 gcgctcgtcg agccggaggt ggaagatagg ttcatggtct tgatccggga gaagcccgtc
     2701 ccggcgaaga ccgtgccgtt gccgttctac aagcgcgaga agcgatagct aagggaggac
     2761 agatttgagc gtaccgggag atcttcagta caccaagtcc cacgagtggg tccgcaccga
     2821 cggagacacc gctacaatcg gcattaccga gcacgcccag gacgagctgg gtgacgtggt
     2881 cttcgtcgag ctgccggaag agggcgcgac cttcgacgcc ggcgactctt tcgggactgt
     2941 tgagtccgtc aaggccgtct ccgacctgta cgcgccggtg ggtggcgagg tggtggaggt
     3001 caacaccgcg ctcgaagaga gccccgagaa gatcaacgaa gacccatacg gtgacggctg
     3061 gatcctcaag ctccgaacct ctggccaggg cgacctgctc tccgcggatg agtaccagaa
     3121 ggtcctggac gaagagtcct agaccgtctc gaccctaccc aaaacccgcg agcaacggag
     3181 attttcgggc tggatcccgc ctccagcacc tgtgtttgcc gcgtatctat gtgcattttg
     3241 cagatatttt caaaaagttt ctaaattctc tgttccaaga cctttacaaa gtgtatctag
     3301 cgaagtatca tcctgtttgt cggttggtgc gaaaccagta ccggcacaat taaagagcct
     3361 ggggcgttac tcctcctttc cctttcccca cctatcaccc ctaagttgcg ttccaggctc
     3421 ttcctctttt ctcacaacct cagtcagcta agttcagcgc ttatcctacg tctcttcaac
     3481 gagtcaactc aagatcttca aggtctcacg cttccttcag ccaacaaaaa ggccccgggc
     3541 agaccagggc ctcaagcttg gtgcttgtcc tcgaccgctt taagaggcct cgcctacctg
     3601 ggtgaggtag tagagggtat gcatgagttg agcggtgggg tcgatgcggt ggacgtccac
     3661 gttgtagggt acgtggagca ggacatcggt gtagttgagg atgacccgga tgccggcgtc
     3721 caccatcagc tcggcgacct cctgagcgga ggaagccgga gtggcgatga tgccgatgat
     3781 ctcctcggcc tccgccacgc tcttctttag ctcgtcgagg tgcttgacga cgacgttccc
     3841 gaccgtgcgt cccgccttct tggggtcgtt gtcgaagacg tcgtggatga cgaaaccgcg
     3901 cttggggagg atggtgctgg cggcgatggc gctgccgagg ttcccggccc ccacgagcgc
     3961 gatgttgtaa gtctgccgca ggcccaggat gctgcggacc gcttcgacca ggtcgtatgc
     4021 ctggtatccg accccgcgcg tgccggagaa gcctatggcg ttcaggtcgc gccgaacctg
     4081 caccgggttt atgccggtga agtcgccaag ctccttgctg gagaccgcgt cacggccctc
     4141 ctctatgagc tgctgcgtgg tacggaggta cttcgtaagc ctattcgtca ggcccggttg
     4201 tagcgtggct cgcacgttag atcctgctcc ttctgtttcg tctgatcgca ttagtgggag
     4261 cattgtaaca aaatggtccg ctcttgcaat aaaggtgctc ttaacagacg acaaatcagt
     4321 aagcaagcta ggttacagag atgtcgatat cggcagacaa cgttacgaag cggggtaaac
     4381 ggcgcgttgc gcggggcttg actgcgtata atctgcattc gcggttcagc ttgtgcgaga
     4441 agaaggatgg ggtctgccgt ggcgacaatt ctctggggtg cgctggtcat tctggtgaca
     4501 gtaggggtta cggtggcggg tatgatcctg gcgcggcggc gggtcccgtt gccgctccgg
     4561 gaggcgcaca ataatacgac cgctaccatc ttcggggcgc tctatgtaat gtacgccctg
     4621 atggtcgggt tctcggcata tttcgtggct taccagttcg atacggccca gaagaacgtg
     4681 gagagcgagg ccggcagcgt ggaggagatc tactacctcg ccgagcagtt ccccgaaccg
     4741 gaccggcgca aggttcaggg gctaaccgag tcctatgcga agaccgtcat acaacaggga
     4801 tggcccatga tgaagcaggg taagttgagt ccgcgcgccg gggagactgc tgacgagctt
     4861 cggcgtactg tcttgagctt cgagcctaag acaagctccc agcaggcggt ctatgcccag
     4921 gcgctgctag tgctccagga cctcgacgag taccgggcgc ttcgtctgtt ggatgcccgg
     4981 acggggatac ctctcatact ctgggtggtc ctggtggtcg gggggatctt gacggtggcc
     5041 ttcacctacc tcttcgggat gaaggaccac cggctgcacc tgctgatggt ggtggcgttt
     5101 accgtggtcc tcatcctgac cctctatact atccgcgccc tcgaataccc cttcgacggc
     5161 atcgtacaga taggatcgga cgcattcgag acggtcctgg ataacatccg ggccggcggc
     5221 gggcagtagg tccgcgctgc gcgtccgggg ccgtggcgag gacggggaat cgaaagatcc
     5281 cctgctggag gatgcgcggt attcgtatcc ttgctatcat tgcggccgtg aacgtggagc
     5341 gcacacggtt tagcaacgca ggtctccccg gcggacggct tcctcgaccg ccgctgcccc
     5401 gaccgtccct ctaggggccg cgctcggggc atccgcatcc ctccgaaatc ttcggggttt
     5461 tcgcgcggaa tagtctccat tcacatcagg gagaatcgag gaaaacaatg ctagcttttt
     5521 cgaccagaga cgatactgac acagaggaga ctggctttcg ggaagccctg cgcgccgcgt
     5581 cgcgggagga cggtggggca agcatccggc ccatgagcga tgtggacctg gatcgcatcc
     5641 tggagctgag gacggttgtg aactgggcgg ccgaccctcg ggcgttcgac ctgttgcgcg
     5701 gcatccggga cgcccggtgg gcggtagcgg agacgccgga cggctcgctg gccgggatgg
     5761 tcggcgcggt gccgttcggc gggatcggga ttctctgcca cctcgccgtt cacgacgact
     5821 accgtaacct tggcctcgga gcgcggctct cggcttgggc gatcctgtat ctccgctcgc
     5881 gcggtgcgag taccgttcgg ctctactcga cccggcgggc cgagaagctg tatcgttcga
     5941 tgggcttccg gcccgccgtg caacgaacca tctaccggct ggacgaagcg tcccggaacc
     6001 cgctggcgcg ccaagaggcc gacggtcacc atgtcgggac gatgctgttc ggggacctgc
     6061 cggagatcta cggcgtagat cactggacct acggcgccga ccgctcggcg ctcatcttcg
     6121 ctaccctgcg gctgcatccc gggcgggggc tcgtcgcgcg ggactccgcg gggcgtgtga
     6181 agggctacct catccgcggc gaagcgggac acgccacagg ccacgcgacg cgcattggac
     6241 cattcgtcgc ttccgaaccc ggtgtggcgc ggctgctact ccagaacgct ctcgacggcg
     6301 cggagggcgc ggctatagag gttacggtcc caggaccggg caacggaccg gcgcacgctt
     6361 tgctgaagga gttcgggttc accggccgcc gcgaccggct gaggatggac ctcggcgaga
     6421 agctctgctc ctgccgctcc gccctcaccc actacggcac aacgccgtac ctggctacat
     6481 agaccggacg cttccgccgc ttgccaggac accggccagg aggtccgggt ccacgttgcc
     6541 gccgcttatg acggccacgt tgcggcgcgt tgggggcagc tcttcccggt ggaacaggta
     6601 ggcggcgaag gacacggctc cgctcggctc ggcgaccatg cggacccgga gggccagccg
     6661 gcgcatggcg tccaggatct cctcctcagt caccgcgatg atgtcgtcca cgaaggcgcg
     6721 gacgtgctcg aacggggcgt tgcccagccg ccgcacccgc agcccgtcgg cgacagtgcg
     6781 gctgacctcc tcggcggaga actctacgat ccggccgctg cgcaggctgg cccgcgcgtc
     6841 ggcggccagc tccggctcta ccccgatcgc gtttatatcc ggccggttta gcttgagcgc
     6901 cgccgcgact ccgcttatga ggccgccgcc gctgaccggg acgagcacg
//
LOCUS       DASSMB010000177        29009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_676367, whole genome shotgun sequence.
ACCESSION   DASSMB010000177 DASSMB010000000
VERSION     DASSMB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 29009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29009
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676367"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1006)
                     /locus_tag="VFJ72_10480"
     CDS             complement(38..1006)
                     /locus_tag="VFJ72_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HET7479925.1"
                     /translation="MRSKRRKTAPEARNLSRRQMLGLVGSATVAAALAGCGTGQSESG
                     GTSTASTGAATEVAAAAGETTGTVAKKTAPSCVLTPELTEGPYFLDGDKVREDITEGK
                     GGVPLRLRTTVVDATECTPIENAAVDIWHCDAGGLYSGFTQASLDAGGAGSGAPPPGQ
                     PPQGGSMGVGPTDDLTFLRGVRLTDADGVAEFATIYPGWYAGRALHIHLKVMVGGNIS
                     GETYEGGHVAHTGQIFFPEDVTDEVAALEPYASHDVALVRHAEDGIFQAAGEGSIVKL
                     TPLADGGSVEDGFTAEISLGVNPETTPDPAGMGAGSSPANSSAATG"
     gene            complement(1112..2092)
                     /locus_tag="VFJ72_10485"
     CDS             complement(1112..2092)
                     /locus_tag="VFJ72_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479926.1"
                     /translation="MIPALLTLLSLLLVGSALGTAALALVSMAASRKPTSSLALPASV
                     LGVLGLTAALMGGRIHLLDLASVAGIVLIGGIAGAGWAPAGRVVLLSAAPLCLLAGLL
                     GRWEAFSYVLSSIGTSGWAPALTDAMNGLAWGVARSAGFAAFLAATGAVLLGTRRPAR
                     LPVGGLPARVYALHRALGIASILALAVHLTSLWLDSFVQFSWAQLLLSPWASSYRPLA
                     VTLGWLAMIALLLTAASGGLRRLLPGWRIVHTLAYLTFALGLVHGLLSGSDTGSPWAI
                     GFYLAALLAVAWTMIRRFSNSRPHHQLDRKKRTAKDLAPASEITGKSQLS"
     gene            complement(2089..2970)
                     /locus_tag="VFJ72_10490"
     CDS             complement(2089..2970)
                     /locus_tag="VFJ72_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET7479927.1"
                     /translation="MTLLTLSARYWSGRVMGTHARLGVVTGDPAAGDTALRRAVEDLE
                     TTERALSRFREDSALSQLNRLGTALPGARLLTAIETAIDAYEWSGGLLDPRIIDPLER
                     FGYRQGVPCGDVGEVEPSGALPPVDMRGWVLDSGRVSLPSGVRLDLAGVGKALGIGWA
                     AMRLTGHEGLLVDVGGDVVALGADENGEPWTVLVMHEEPVGQFSGSSLAVATSTTRRR
                     AWTAGGNEAHHLIDPRTGAPSAGELSYATVAAPTILEADLAAKLLILEGPSALERFDD
                     GYRAITTDRHGKTEVFA"
     gene            complement(2967..3494)
                     /locus_tag="VFJ72_10495"
     CDS             complement(2967..3494)
                     /locus_tag="VFJ72_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479928.1"
                     /translation="MTDKSTPENQGRPREGFETRSPGSGGPRKKAGRVSQRRELAIVT
                     LGMAASIAGLGGLLAANPPSWAVSQADVRQAITTEAAVTPGQSASTQNNAIRPSSGTD
                     DDSAGASAGARSDADRDEVETVQASAQQQASRQAARREARRAARQSLRQPAQQSTPVY
                     SAPTQTSPSAVSQGS"
     gene            complement(3528..3929)
                     /locus_tag="VFJ72_10500"
     CDS             complement(3528..3929)
                     /locus_tag="VFJ72_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479929.1"
                     /translation="MESISRKIPVTGSSKRSTLWGAGLMSLIALIHLVVIPEYAGFAA
                     YLGVLFFANFIGSGLSAVGIYRRTWWGWPLGIVMAGGAFIMYIESRTIGLPGLNEGWF
                     DPPGVLSLILEAAFATLFLRGAFRQGASEAR"
     gene            4063..4755
                     /locus_tag="VFJ72_10505"
     CDS             4063..4755
                     /locus_tag="VFJ72_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479930.1"
                     /translation="MCEMARILIIEDEEKMARMLSRILREEGYVAETAGDGRTGLSRA
                     LDDSFDLLIVDWMLPERSGVQIVRGLRAADIHTPVLMLTARDQVEDRVEGLDAGSDDY
                     LSKPFALPELLARVRALTRRPFAAGDGGKEIRAGDIVLDPVRHVVRRGEERIDLTAKE
                     FALLAALMQRPGQVFSRSVLLDTVWGVPGEVSTSVVELYVSYLRRKLDRDGEPSRIRT
                     VRGVGYTFEPRP"
     gene            4774..6201
                     /locus_tag="VFJ72_10510"
     CDS             4774..6201
                     /locus_tag="VFJ72_10510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7479931.1"
                     /translation="MIERIRRRLTLGYVGILALILLLFGAIVVGIFSAQLNRQQDEQL
                     TRQAQDVAGFVRNHGPGGAEADEPTPDRSAPSAPKTPGLAQDGPRLRHDDGSGIAVYV
                     VPPKTLPDGADAGEEGIYGKVSNPGVPNTDLGLPFTGAAREAEREASRNGARGDGPGG
                     GAAGGPGDGSDAETLPGPDGGVRVVSVPVIGESGQTIAVVQAAQSRRAVDETVEDLLL
                     ALVPVGVASLLLAGVGGLFMSRRAMQPVKDSFQRQRTFIADASHELKTPLALVKVGAE
                     VMRRNPADPENREVIEDQLSEIDRMNALLSDLLVLARLDAGRLDVEDKPFDLSVIAAE
                     TAGRFLTRAANESVRMEVEVPDRLPARGDSDRTAQILTALLDNAMRYTPEGGTITVSG
                     RRLGDGVEASVSDTGPGVPAEHLDRIFDRFYRAEEARTRAGGGTGLGLSIARDLARAQ
                     RGDLSAQNVAEGGTGAIFRLKLPAQ"
     gene            6269..6613
                     /locus_tag="VFJ72_10515"
     CDS             6269..6613
                     /locus_tag="VFJ72_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479932.1"
                     /translation="MRVMKAAAVLNHWLAWRRRSLANRVLFALSLSLAGFVTGAFTWA
                     ALNPDPGNAARYFFFYWIWGLCAVGVLLGMAEYKTSPLRKGRERGHLFILLYIGALLI
                     VTRALNMVLFYG"
     gene            6606..7265
                     /locus_tag="VFJ72_10520"
     CDS             6606..7265
                     /locus_tag="VFJ72_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HET7479933.1"
                     /translation="MGEGAYRAVLFDLDGTMVETESLHLPTWTEALRPHGIEVDEVLY
                     RDRISGRGNAKIVGDLLPDLSDEEGRAVAEAKEADFRERAVDLEPLPGLVEFLEEVRR
                     RGLDTAVVSNAPRENAAAVLRALGLDGGFGLIVLADDVGMGKPDPAPYRAAVERLGIA
                     PSRALAFEDSVSGIASAVGAGIPTVGIAFTNDAAALREAGAFEVVEDFTDPRLRGLLD
                     R"
     gene            complement(7228..8151)
                     /locus_tag="VFJ72_10525"
     CDS             complement(7228..8151)
                     /locus_tag="VFJ72_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7479934.1"
                     /translation="MRRAVLATLVGAALLVAAYMGVGLLVALRLSAPERSPEEGTPAD
                     VGLRYRDVGFESEDGVPLRAWWVEKRDAERAAILVHGFGGNRSEEHILKSARIYASKG
                     YSVLMLDLRGHGASGGERRTLGYREVRDVRAALSWLEQRGYGPHEVVLHGWSMGAATV
                     VRAAPGTGVAAVVEDSGYADLPLLLGKELPENSGLPSLFNPGIMLSAKLFLGFDPWSV
                     VPANEAAQLRRQDVPLLVIHSTTDETVAYEHARIFLRADPRAELWRLEGYDHVQSYTN
                     PDYRKKLTAFLDRTAPQEDSSGPATLAVAGP"
     gene            complement(8161..10581)
                     /locus_tag="VFJ72_10530"
     CDS             complement(8161..10581)
                     /locus_tag="VFJ72_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HET7479935.1"
                     /translation="MTETYEKPPETGGDYEPLGSAELMDHGDRGLRLKAGSTTIEITA
                     LAPDLFRVGAFPHGRTPRYDTEAIARDDWGTVEVAMEQSDEEIALKTPAATAHISLHP
                     LRIHFTDAEGNEFAADDPDLGMGFVPQPGADVFSSPLGEPARLYKKRQEGERYFGCGE
                     RTSGLEKTGSYQVFWNVDPPAGHTASFENLYTSIPFTLSIIDGRARGLFFDNTHRVEF
                     DLAKESADRAYYGAEGGDIVYYAFAGPTPKQVLDRYTELTGRTPMPPMWALGNQQCRW
                     SYETADEIRELAREFRDRDIPCDVIYLDIDYMDGYRVFTWDRDAFPDPEGLLADLREQ
                     GFRVVTIVDPGVKIDEDYPVYVEGRARDLYCKTQAGEEFHNVVWPGMCAFPDFTNPET
                     REWWGENHRALTDVGIAGIWCDMNEPSLFVPLQSTMPGNVVHPGGGDPKWHAQIHNTY
                     GSLMARSAREGLLKLRPDERPFVITRAGYAGLQRHAMQWTGDNSSWWEHLWMSMPQLQ
                     NLGLSGVGWAGVDIGGFFGDSNGELLARFTEFGVFQPFCRNHSAKGTINQEPWSFGEP
                     YESVCRKMIKLRMRLLPYLYTLFEECHRTGAPILRPLLFEYPEDETTYTADDEFLLGP
                     SLLVAPITRPGIEHRHVYLPEGTWFHYWTGERFDGPQHILAHAPVGEPALYVKANSAV
                     PMGPEVSHTDEKPDTLSFLLYPAEGSGESALYEDAGNGFGYQSGDYARRSVTCETNAQ
                     SVTVRVGEREGSFEPEPRDLLLELRGLDAVQSVLVDGEEREPRRDEGGIVVPLPETAN
                     AVTMEVRV"
     gene            complement(10689..11831)
                     /locus_tag="VFJ72_10535"
     CDS             complement(10689..11831)
                     /locus_tag="VFJ72_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7479936.1"
                     /translation="MNGTGIRAARLSVLAVFFLNGLAVASWFVRIPAVRDNLGLSEGL
                     LGVALLGAAVGAMVAMPLAGALTSRFGSRRVVGVTALLISFSLIPPALAPNLFFLVPA
                     LVLLGASNGALDVSMNSQAVAVEKEYGRPIMSSFHAAFSLGGLAGAASGGLVAAAGIG
                     PLPHLSAVAVLCASTALVAYRPLLPASADAAGDGAPAFARPTRALLGLGVISFCVLLG
                     EGAMADWSAVYLKDTLTTGPGFAAAGYAAFSLAMAFGRLLGDRLTQRLGPAALVRACG
                     ALAAVGLGVALAVGQPYFALVGFACAGAGFSVIFPTALSAAGRTKGMAAGPALAAVST
                     AGYTGFLIGPPTIGFVAELVGLGGALYIVVGLSAAIVLLAGAVRGK"
     gene            complement(11828..12319)
                     /locus_tag="VFJ72_10540"
     CDS             complement(11828..12319)
                     /locus_tag="VFJ72_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479937.1"
                     /translation="MVGYMSLEEQVDRDFERARRRSLVAWIRARLRGDVAREGIMLFD
                     ESRRSARADNRVYLGRRVVEVSRIVGSVGRHREFDRDFMPTSASAERWKRVDRAFQSG
                     VDLAPVSLYQIGGDYYVHDGNHRVSVAHYHGVEWIDAEVTRFRGQPNRDAGACGACDR
                     TAA"
     gene            12381..13481
                     /locus_tag="VFJ72_10545"
     CDS             12381..13481
                     /locus_tag="VFJ72_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7479938.1"
                     /translation="MGVKSDGREARRRATLKEVASELSVSPATVSNAYNRPDQLSAEL
                     RERVFETARRMGYTGPDPAARSLRRRRAGAVGVLYTDRLSYAFADPVSVMFLEGIATA
                     TEGAGLGMLLIPGGALRERDPEAIGNAVVDGFVIYCMAENDPLATAALDRQLPTVLVD
                     QPEIGGIPSVGIDDEGAARAAAAHLIELGHRHLGVVSFELALNAPGGIADRRRQSEAT
                     YRPSRSRLKGYASAVEDADRSWDEVPVYECTENAPSQGRIAAEYLLSREPRPTALLCL
                     SDQLAVGAYEAAREMGLSVPEDLSVVGFDDIPAAARMDPPLTTVYQPHVEKGVRAGEL
                     LISQLRGEESSVSELLPARLVVRGSTAPPSKS"
     gene            complement(13478..14428)
                     /locus_tag="VFJ72_10550"
     CDS             complement(13478..14428)
                     /locus_tag="VFJ72_10550"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HET7479939.1"
                     /translation="MTLLLTELEVEELLDMPSVLDAVEEILRQHAEGRATNRARRRVA
                     LPTAGLNVMFAGAPEIGALGLKAYTVARTGARFYTMLFDPDDGDLLAIIQSDKMGQLR
                     TGAATGVATKYLAREDASTLGIYGAGWQAESQLEAIASVRDLERVIVYSRSEEKRKSF
                     AEKMGERLGMEIETTHSPEEPAAQDIVVTVTSSKEPVLYGEWLRPGTHVNAAGSNMLF
                     KSEVDREVIKRASFVCVDSREEIGLEAGDLMQPLETGLILPEAVHELGQVIAGRVPGR
                     RGPEDITFFTSQGLALEDMAAARIAYDRAMERDMGRDIEF"
     gene            14480..15046
                     /locus_tag="VFJ72_10555"
     CDS             14480..15046
                     /locus_tag="VFJ72_10555"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET7479940.1"
                     /translation="MRVLVIDNYDSFTYNLVQYLGELGAEVIVRRNDELTPKEVLDLH
                     PDRIVVSPGPCTPNEAGISVDLISESAAAEVPLLGVCLGHQSIGQAFGAKVVRGEPVH
                     GKTARILHDGEGVYAGVEQGFEATRYHSLVIEPESLPECLVVTSRTEDGVIMGVRHRE
                     YPVEGVQFHPESIMTRSGHDLLKNFLDG"
     gene            15039..16091
                     /gene="trpD"
                     /locus_tag="VFJ72_10560"
     CDS             15039..16091
                     /gene="trpD"
                     /locus_tag="VFJ72_10560"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET7479941.1"
                     /translation="MAEVPVLRESLRKVAAGEALSQGEAERALETIMEGTAPPAATAA
                     LLTAMRIRGESVGEIVGFARAMRRFAAKVEAPEDVVDTCGTGGDAKGTINISTAAAFV
                     ARGAGVTIAKHGNRAATSLAGSADVLEALGAEIELGPEQVSRCIAEAGIGFMFARTHH
                     PAMRFVAPVRAELPFRTVFNLLGPLTNPAGAKRQLIGVFSGDYVRPVAEALKELGAEK
                     ALVVHGADGMDEITVSGPTRVAEVSDGEIEEYDVTPEDFGLPRHAPDGILGGDAHLNA
                     RILRDILSGEEPGAPRDVVLMNAGAAVFVSGGAGTLDEGVRLAERSVDSGAALEALET
                     FVKITRRLAAGPGGTR"
     gene            16088..16855
                     /locus_tag="VFJ72_10565"
     CDS             16088..16855
                     /locus_tag="VFJ72_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase"
                     /protein_id="HET7479942.1"
                     /translation="MSIPTVLDDLAAAARRRADELKTNTDLDALYERAIRFEKRDFAA
                     ALRSPGLSVISEIKRASPSAGFIRDADPAEWGARYESEGASCLSVLTEPDRFKGSLKD
                     LDAARENTTLPVIRKDFTVDEVHVIEAATRADAVLLIAALFDATSLSRYVALSIEVGL
                     TPLVEVHDEREAEIALECGATVIGVNNRDLRDFTVDLGTFERLAPGLDGATLVAESGV
                     KTPEDAGRLRDAGADAVLVGEAAMRDPSLVAKLSALP"
     gene            17017..17421
                     /locus_tag="VFJ72_10570"
     CDS             17017..17421
                     /locus_tag="VFJ72_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7479943.1"
                     /translation="MDDRTAVTQVLTDYYNAFGTLDVQRILSYYHEPCMLVSPQGVAA
                     LPSRAAMAAAFTPIMEGLRARGYSRSESSMLHVKELSATAALASGIAVRYRADGQELE
                     RIGVSYLLHKTDDRWKIAVTVVHDTDNVVRSE"
     gene            17679..18434
                     /locus_tag="VFJ72_10575"
     CDS             17679..18434
                     /locus_tag="VFJ72_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479944.1"
                     /translation="MTTTANINKMTRTTREIAEVQRDSFEALAENFSDLQRRNIEFAQ
                     GGFEFIRLQEDNAKAAREWFANGLKLAKLQQRNVEFTQKWMSGGVELLRDQAEHNRRA
                     VEVFSQSTRKQQENFRALAEEWTGAYRNFFSPFAYAQEGLKAAQQTTQQAVQATQQVA
                     QQGLRVAEETTRKSEQAIRKTQEATREAALQTAVHSALKTANYEDLNVDEVSKKLDGL
                     SAAELKKVREYEKKNKNRETLIEQIDRKLKATS"
     gene            complement(18563..18760)
                     /locus_tag="VFJ72_10580"
     CDS             complement(18563..18760)
                     /locus_tag="VFJ72_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479945.1"
                     /translation="MESAVVYQRLLREGAEVPDYAMSAVLNAFRVGGLINVPIRDPRE
                     EEVRKHGDLSINGVSPLLCSV"
     gene            complement(18959..19687)
                     /locus_tag="VFJ72_10585"
     CDS             complement(18959..19687)
                     /locus_tag="VFJ72_10585"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7479946.1"
                     /translation="MRSIVPHPVLLTLTLILIAGAISFVQIRFKAADASDQRTQSASV
                     KPSNTEANGPSRTAGLRTVPEQGKEDSAEQERSKDESPIRGESIAQKQARYPRAHEIV
                     DPTGFINTDGISLADARGKVVLLEFWTYSCYNCQNAQPHINRFYERYDGEGLQVIGIH
                     KPEFEFEKDYASVSEAVKDANIQYPVALDNNDATWNAYSQRYWPTWYLIDADGFVRYK
                     HIGEGAYDETEKKIQELLAERDLH"
     gene            complement(19684..20385)
                     /locus_tag="VFJ72_10590"
     CDS             complement(19684..20385)
                     /locus_tag="VFJ72_10590"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7479947.1"
                     /translation="MTQPTLVLALAAGLASFLSPCMLPVIPAFLAQLAGASLGVTSPK
                     RRYLLLSTALFVAGFSTVFALLGIVLNAVLQQGAAEVLAWLSRTAGAVIIVLGLHLTG
                     LLRLPILDRQYSVDPGNSRPGYLTSLLFGASFAVAWTPCVGPILGSTLALAASEPAAA
                     FPLLLAYATGLGTPFLLVGLFPSGAFALIRKHRSWAARLHTVFGGVLIGMGVLVFTDK
                     LSLLANFQLLNEVLL"
     gene            complement(20539..21774)
                     /locus_tag="VFJ72_10595"
     CDS             complement(20539..21774)
                     /locus_tag="VFJ72_10595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7479948.1"
                     /translation="MIKRLLAGLLVLFAGLAVTVLVSAALFGIPAADVPDIALLLVGA
                     GGAAGLVALLLVRPAVLGRLGGVRAQLVGAGLIGSLLLLGMMLAGARAMFISDHDFAV
                     VLTMLLFAAILSVGFSLLFAAPLANRIERVRLGAARLTNGDLDTEIPAEGHDELTALA
                     RDFNSMARSLKESAEREREMEKARRELIAAISHDLRTPLAATRALVEALVDGVAAGPE
                     TEARYLESARRELMHLSQLVDDLFELSRIDAGVLKLELEGGSLHDMISDTLSSFQPQA
                     ERQNVRLVGEVSEGVDPVLINPPRLQRVLHNLVSNALRHTPSDGTIALRAKPIGSEVQ
                     VEVADTGEGIAPEDLPRVFERTFRGEKSRTRSGDDGDSNAGLGLAIARGLIEAHGGAI
                     EVESHPGGGSRFRFTLRRA"
     gene            complement(21780..22481)
                     /locus_tag="VFJ72_10600"
     CDS             complement(21780..22481)
                     /locus_tag="VFJ72_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479949.1"
                     /translation="MVAKVLVVDDEPNIREVVGLYLSRDGHTVVSAADGVEALEVFRR
                     SEPDLVVLDLMLPRINGLEVCRRIQAERRVPLIMLTARGEEEERIVGLSLGADDYVVK
                     PFSPRELAARVAAVLRRVEESGPDSGRKPGQKVLSFEGLRVDPNRREVLVRDEPVTLT
                     AREFDLLYYLASSPGRVYTRDHLMETVWGYAFAAETSTVTVHVRRLREKIEADPTHPR
                     YLQTVWGVGYKFGRA"
     gene            complement(22604..23680)
                     /gene="msrA"
                     /locus_tag="VFJ72_10605"
     CDS             complement(22604..23680)
                     /gene="msrA"
                     /locus_tag="VFJ72_10605"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7479950.1"
                     /translation="MRFLFNRHKSTMVPPEDALPGRDQPAFAVPERHAVLGTPIKPPF
                     PEGLETAVFGLGCFWGAERVFWKTEGVYTTAVGYAGGYTPNPTYGEVCGGRTGHTEAV
                     LVVFDPRKVTYRELLAVFWEAHDPTQEMRQGNDVGTQYRSAIYYANDEQRGAAEKTRA
                     AYGKKLKEAGYGPIATEIAPTGPFYYAEDYHQQYLHKVPNGYCGLGGTGVSCPVGLGV
                     GTIAEPNGALKPVASSDEDWRERLSPEQYAVLREAGTERPFSGEYVDTDDDGLYHCAA
                     CGNPLFDGRTKYHSGTGWPSFTEAVSPDAVELVEDHSHGMVRTEVRCARCHSHLGHVF
                     DDGPRDAGGKRWCMNSLALDLERR"
     gene            complement(23690..24529)
                     /locus_tag="VFJ72_10610"
     CDS             complement(23690..24529)
                     /locus_tag="VFJ72_10610"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7479951.1"
                     /translation="MTTLRVGDAFPDFELPDHRKVPRRLSRFTHPSAMDERLGFTDGY
                     PVIVVFGRGFFCPRDQEQMRVLVRFQSELAVNYCKLVTVSADPPMVGAAFRVGLGAEW
                     PFLSDEDREVIKGIGILDETEGEYAYRARPFTFVLRPDLTVHKVYDGWFFVGRPTAEE
                     LRHDLREIMSRRRDYLYEAFDTPEAKSIRIPQQEWADGAPPLGANGLPVASGRVKTFD
                     LASGSGEISRDGDEDVFFNFTAIPGEGYRTIRPGTPVRFEVVEHANTGPTARNVQEDR
                     TTR"
     gene            complement(24534..25925)
                     /locus_tag="VFJ72_10615"
     CDS             complement(24534..25925)
                     /locus_tag="VFJ72_10615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HET7479952.1"
                     /translation="MTETQHYDAVIVGSGQGGTPLAKALAGAGRRTAIIEREHVGGTC
                     VNVGCTPTKTMVASARVAYLDRRSPDYGVQDGPVAVDMTKVRQRKRDIVERFRSGGEK
                     GIESTENLDLLVGEARFTASKELEIRTNGVGTLTVTADEVFINVGARPGGAPIDGLDN
                     VPFLDSTSIMELGEVPDHLLVLGGGYIGMEFAQMFRRFGSEVTVVQRGKQLLAREDAD
                     VAEAVADVLREDGIEVLLQTEAQRVENGSGGIELTVKNEGGERVLEGSHLLVAIGRPP
                     NTDSLDPRAGGIETDGRGFIQVNDRLETTAPGVYALGDVKGGPAFTHISYDDFRVLKT
                     NLLDGGNASIADRIVSYTVFIDPQLGRVGLNETEAREQGLDFRVAKMPMSRVARALEM
                     DETRGFMKAMVDAETDRILGCAVLGIEGGEIMSLIHVAMIGKVPYTALRDGAFAHPTL
                     AESLNNLFASFVE"
     gene            complement(26011..27378)
                     /locus_tag="VFJ72_10620"
     CDS             complement(26011..27378)
                     /locus_tag="VFJ72_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HET7479953.1"
                     /translation="MDTTHDSKTDLQPKDLAARAERVLRDNDMGVWTKAAPSLYPHQW
                     SWDSAFIAIGIAHYDTARAAEEIRCLLDHQWATGKVPHIVFNPDAPPESYFPGPEHWA
                     SAAPNAPAAPAKPATTSGLCQPPVHAIAARRILEIAERRDGDAAGAREFLREIYPKLL
                     FWHRYLMTARDPEESGLVTIYHPWESGTDNSPRWQDALDRVEVREKLEYERRDLQHVD
                     DPSERPTQEVYDRFIWLVQLITRSGCDDAVVYATSPFLVKDVLMSAILVRANEALLEI
                     SELVGAPDEDRNVISAWISRGRRGLTKSWDPEIGLCLDYDLRAGAPLKARTVAGFAPL
                     IAGIETGRRSLLQTLDSALFAGHSKLRWPLPPSTSPEEPLFDPRRYWRGPVWPVINWL
                     LWWSLERSGEDERAARLRRDGLAQLADGTFSEYYEPFTGEPLGSDRQSWTAAVLLDWL
                     ANGQM"
     gene            27828..28445
                     /locus_tag="VFJ72_10625"
     CDS             27828..28445
                     /locus_tag="VFJ72_10625"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7479954.1"
                     /translation="MVLVKVCGITNPEDARVAADAGADAIGIVFAPSPRRVSVEDARK
                     VAGTLPDDVLKVGVFVDAAPEELLRVAGEVGLDYAQLHGDEGPEDVTHLRKNGLKIIK
                     ALRVRDAASLEALEEYGADLYLLDAYSEKARGGTGERFDWRLAKTLRARDNIVVSGGL
                     NPENVREAIEFFEPYGVDASSSLEDRPGVKNGERVRRFVSAAKSR"
     gene            28426..>29009
                     /locus_tag="VFJ72_10630"
     CDS             28426..>29009
                     /locus_tag="VFJ72_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7479955.1"
                     /translation="MRQKVDSGYFGPYGGRYVPETLIHALDELEDAYERYKNAPEFLE
                     ELDGLARDFVGRPTPLMFAGRLTRKWGGANVWFKREDLAHTGAHKINNALGQMLLAER
                     MGKTRIIAETGAGQHGVATATVAALYGKECVVYMGEEDTRRQAPNVLRMKLLGTEVRP
                     VTSGSGTLKAALNEAIRDWVANVEDTHYIIGTVAG"
BASE COUNT         4758 a         9439 c         9909 g         4903 t
ORIGIN      
        1 cgccgcggac cacggtcccg ccgccaaccc ggccttttta accggtcgcc gcgctgctat
       61 tggcggggct gctcccggcg cccattccgg cggggtcggg ggtcgtctcg gggttcacgc
      121 ccagcgagat ctcggccgta aacccgtcct cgaccgagcc gccatccgcg agtggcgtga
      181 gcttcacgat ggagccttcg ccggccgcct gaaagatgcc gtcctccgcg tgccggacta
      241 gcgctacgtc gtggctggcg tagggttcga gcgcggcgac ctcgtcggtg acgtcttcgg
      301 ggaagaagat ctggccggta tgggcgacgt gcccgccctc gtaggtctcg ccggagatat
      361 tgccgccgac catcaccttg agatggatgt gcagggcgcg gcccgcgtac cagccggggt
      421 agatggtggc gaactccgcg acgccgtccg cgtccgtcaa ccgcacgccg cgcaggaacg
      481 tcaggtcgtc cgtcggtccc acgcccatgc tcccgccctg cggcggctgt cccggtggtg
      541 gcgctccact acccgctccg cccgcatcta ggctcgcctg cgtgaagccg gagtacaggc
      601 cgccggcgtc gcagtgccag atgtccacgg cggcgttctc gatgggcgta cactctgtcg
      661 catccacgac ggtcgtccgc agccgcagcg gcacgccgcc tttgccctcg gtgatgtcct
      721 cccggacttt gtccccgtcc aggaagtacg gtccctccgt tagctcgggg gtgagcacgc
      781 acgaaggagc cgtctttttc gctacggtcc ccgtcgtctc cccggcagcc gccgctactt
      841 cggtcgccgc cccggtggac gcggtgctcg ttcctcccga ttcggattgc ccggtaccgc
      901 agcccgcgag cgccgcggct accgtggcag atcccacaag gccgagcatc tgccggcggc
      961 taaggttgcg agcttctggt gctgttttcc ggcgtttgga tctcatcgta ttttcctcct
     1021 aacgttcttg tctactggtg tatttctgag ggcgcttcag cggccgttgg gatcatcaag
     1081 cataactggt cccttgcgaa cggcgaaagg gttagctgag ctggcttttg cccgtgatct
     1141 ccgaggccgg cgccagatct ttggcggtgc gcttctttct gtctagttgg tgatggggcc
     1201 tggaattaga gaacctgcgg atcatggtcc atgccaccgc gagcagcgcg gccaggtaga
     1261 agcctatagc ccagggagag ccggtatcgc tgccggagag caggccgtgg acgagcccca
     1321 gcgcgaaggt gaggtaggcc agggtgtgca cgatccgcca gccgggaagg agacgccgga
     1381 gaccgccgct cgcggccgtc agcagcaggg cgatcatcgc cagccagccg agcgtgaccg
     1441 ccaggggccg gtaggacgag gcccaggggg agaggagcag ttgcgcccag ctaaactgca
     1501 cgaatgagtc gagccacagc gaggtgaggt gtacggcgag cgccaggatg gaggctatgc
     1561 cgagggcgcg gtgcagcgcg tagacgcggg ccgggagccc tccgaccggg agccgggcgg
     1621 ggcggcgggt gccgagcagt accgcccccg tcgcggccag gaaggcggcg aagccggccg
     1681 agcgcgccac gccccaggcc agcccgttca tggcgtcggt gagcgccggc gcccagccgg
     1741 atgtcccgat ggaggagagg acgtacgaga aagcttccca ccggccgagc agcccggcga
     1801 gcaggcagag cggcgctgcg gacaggagga cgacgcgccc ggcgggagcc cacccggcgc
     1861 ccgcaatgcc tccgatcagg acgatacccg ccaccgacgc gaggtctagg aggtggatcc
     1921 ggccgcccat cagggcggcg gtgagaccga ggacgccgag gacggaggcc ggcaatgcca
     1981 gagatgaggt gggcttcctg gaggctgcca tcgagacgag cgccagtgcg gcggtaccca
     2041 gggcggagcc gacgagcagt agtgagagca gagtgaggag cgccgggatc acgcgaacac
     2101 ctccgtcttt ccgtgacggt cggtagttat ggcgcggtag ccgtcatcga accgttcgag
     2161 ggcggatgga ccctccagga tcaggagttt cgcggcgagg tccgcctcca ggatggtggg
     2221 agcggcgacg gtggcgtagg atagctcgcc cgccgaggga gcgccggtcc tcgggtcgat
     2281 cagatgatgc gcctcgttgc cgcccgccgt ccacgcccgg cgccgcgtgg tcgaggtggc
     2341 gacggcgaga gaggagccgc tgaactgtcc cacgggctcc tcgtgcatga cgagcactgt
     2401 ccagggctcg ccattctcat ccgcgccgag ggcgaccacg tcgccgccca cgtccacgag
     2461 cagaccttcg tgtccggtca ggcgcatcgc cgcccatccg atgccgagcg ccttgccgac
     2521 gcccgcgagg tccagacgca caccggacgg cagggagact ctaccggaat ccaggaccca
     2581 gcctcgcatg tccacggggg gcagcgcccc agacggctct acctctccga catcgccgca
     2641 ggggacgcct tgccggtagc cgaacctttc gaggggatct atgatccgcg ggtccagcag
     2701 cccgccggac cactcgtagg cgtctatagc cgtctcgatg gccgtcagca gacgcgcgcc
     2761 tggaagagcc gttcccagac ggttgagttg cgagagcgcg ctgtcctcgc ggaacctgga
     2821 gagagccctc tccgtggtct ccaggtcttc tacggcgcgc cgcagcgcgg tgtctccggc
     2881 tgccgggtct ccggtcacga ccccaagccg cgcgtgggtc cccatcacac gcccgctcca
     2941 gtaacgagcg gagagcgtca agagcgtcac gatccctggc tcactgcgga gggcgaagtc
     3001 tgcgtcggag cggagtagac cggtgtggac tgctgggccg gctgccggag actttgccgg
     3061 gcggccctgc gcgcctctcg ccgggccgct tgccggctgg cctgctgttg cgcggacgcc
     3121 tggacagtct cgacctcgtc ccggtccgca tcggagcgcg cgcctgccga ggcgcctgcc
     3181 gagtcgtcat ctgtgcccga tgacgggcgg atagcgttgt tttgcgtaga ggcggattgt
     3241 ccgggagtga cggcagcctc ggtggtgatg gcctgccgga catccgcctg ggataccgcc
     3301 cagcttggcg ggttcgccgc cagaagaccg cccaggcccg ctatcgaggc ggccatcccg
     3361 agggtgacta tggcgagctc gcggcgttgc gagacgcggc cggccttctt ccttggccct
     3421 ccgcttccgg ggctccttgt ctcgaagccc tcgcggggcc ggccctggtt ctctggtgta
     3481 ctcttatcgg tcatcgtgct cctcttctgt gggctaccgc gccggtgtca ccgggcctcc
     3541 gaggctccct gccggaatgc tccgcgcagg aacagcgtcg cgaaggcggc ttcgaggatg
     3601 agggagagta cgccgggcgg gtcgaaccat ccctcgttca gtccgggcag gccgatagtg
     3661 cggctctcga tgtacatgat gaaggcgccg ccggccatca cgattccaag cggccatccc
     3721 caccacgtcc ggcggtaaat gcctacggcc gagagaccgg agccgatgaa gttggcgaag
     3781 aacaggacgc cgaggtaggc cgcgaaaccg gcgtactcgg ggatcacgac caggtgaatc
     3841 agggcgataa gcgacatcag ccccgctccc cacagggtag acctcttgga cgaaccggtg
     3901 accgggatct tccggcttat gctctccatc ctctgctcct tctgtcgcgg atgcgttacc
     3961 cggtaagctt ggatcaccag cctatgagcc gccttcgaga acgctatgag ttctcatagg
     4021 aacgggtctc attgcgctac gtggagcggg aggtgggcta gtatgtgtga gatggcgcgt
     4081 atcctgataa tcgaggacga ggagaagatg gcccggatgc tgagtcgtat ccttcgtgag
     4141 gaaggctacg tcgcagagac ggcgggggac ggccgcaccg ggctctcccg cgccctggac
     4201 gactcgttcg acctcctgat cgttgactgg atgctcccgg agcgcagcgg cgtgcagatc
     4261 gtgcgcggct tgcgggccgc ggacatccat acccccgtat tgatgctgac cgcccgcgac
     4321 caggtcgaag accgggtgga gggcctggac gccgggtcgg acgactacct ctcaaagccc
     4381 ttcgccctgc cggagcttct ggcgcgggtc agggcgctaa ctcgcaggcc gttcgcggcg
     4441 ggcgatgggg ggaaggagat ccgggccggc gacatcgtcc tggacccggt tcgccacgtc
     4501 gtccgccggg gagaggagcg gatagacctt accgccaagg agttcgccct actcgcggcc
     4561 ctgatgcagc gtcccggcca ggtcttctcg cgctcggtac tgctggacac cgtgtggggc
     4621 gtgcccggag aggtctcgac gagcgtggta gagctttacg tctcttacct gcgccggaag
     4681 ctcgaccggg acggggagcc ttcacgcatc cgcaccgtcc ggggcgtggg ctacactttc
     4741 gagccccggc cttgaggtag acgaggtaga ctggtgatcg aacgtatccg ccgccgtctc
     4801 accctgggct acgttgggat tctcgcgctt atcctgctcc tcttcggcgc aatagtggtc
     4861 ggtatattct cggcccagct caaccgtcag caggacgagc aacttacgcg ccaggcacag
     4921 gacgtggccg gcttcgtgcg gaaccacgga cccggaggcg ccgaagcgga cgagccgacg
     4981 ccggaccgtt ccgcgccgag cgcccccaaa acgccggggc tggcgcaaga tggtccccgc
     5041 ctccggcacg acgacgggtc cggtatcgcc gtttacgtgg tgccgccgaa gactcttccc
     5101 gacggcgcgg acgccggcga agaggggatc tacggcaagg tctcgaaccc tggtgtcccg
     5161 aatacggatc tcggcctccc attcaccggc gcggcgcgcg aagccgaacg ggaagccagc
     5221 cggaatggcg cgcggggtga cggaccggga ggtggagcgg caggagggcc gggggatgga
     5281 tcggacgcgg agacgcttcc gggtccggat ggcggagtgc gggtcgtgag cgtccccgtg
     5341 atcggagagt ccggccagac gattgccgtc gtccaggccg cgcagtcgcg gcgggccgtt
     5401 gacgagacgg ttgaggacct gctcctcgcc ctcgtcccgg tcggggtcgc gagcctgctt
     5461 ctggcgggcg tgggcggcct ctttatgtcc cgccgggcga tgcagccggt gaaggactcg
     5521 ttccagcgcc agcgtacctt catcgcggac gcctcccacg agctgaagac ccccctggcg
     5581 ctcgtcaagg taggcgcgga ggtgatgcgg cgcaacccgg ccgaccccga gaaccgggag
     5641 gtcatcgagg accagctctc cgagatcgac cggatgaacg cgctgctctc cgacctgctc
     5701 gtccttgccc gcctggatgc cggccggctc gacgttgagg acaagccctt cgacctctcg
     5761 gtcatcgcgg ccgagaccgc ggggcgcttc ctgacgcggg ccgcgaacga gagcgtccgg
     5821 atggaggtcg aagttccgga caggctcccg gcccgcggcg actcggaccg gaccgcccag
     5881 atcctgaccg ccctactgga taacgccatg cgctacacgc cggagggagg gaccataacc
     5941 gtctccggcc ggcggctcgg tgacggcgtg gaagcgagcg tctcggacac tggccccgga
     6001 gtgcccgcgg agcacctgga ccgcatcttc gaccgtttct accgggcgga ggaggcccgc
     6061 acccgcgcgg gcggaggcac cggactgggc ctctctatcg cccgcgacct cgcccgcgcc
     6121 cagcgcggag acctctcggc gcagaacgta gcggaaggag ggaccggcgc catctttcgt
     6181 ctcaagctgc ccgcgcagta gctccacggg tgagagaggg ctcacggcac gccggcgtcc
     6241 ggctgctaac attgcctcta tgaaagccat gagagtcatg aaagcagcgg cggtcttgaa
     6301 ccactggctt gcctggaggc gccggagcct tgcgaacagg gtcctgttcg cgctctcgct
     6361 ctctctggcg ggcttcgtca cgggggcctt cacgtgggcg gcgctcaacc cggaccccgg
     6421 caacgcggcg cggtactttt tcttctactg gatctggggt ctgtgcgccg tcggggtcct
     6481 gctcggtatg gccgagtaca agacaagccc gctgcggaag ggccgggagc ggggacacct
     6541 gttcatcctg ctctacatcg gggcgctcct gatcgtgacc cgcgccctga acatggtcct
     6601 cttctatggg tgagggcgcg tacagggcgg tcctcttcga cctggacggc acgatggtcg
     6661 agaccgaatc gctgcacctt ccgacatgga cggaggcgct caggccccac ggtatcgagg
     6721 ttgacgaggt cctctacagg gaccgcatta gtggccgggg caacgcgaag atagtggggg
     6781 acctcctgcc ggacctctcg gacgaggagg gccgcgcggt cgccgaggcg aaggaggcgg
     6841 atttccggga gcgcgctgta gacctcgaac ctctccccgg cctcgtggag ttcctggaag
     6901 aagtccggcg gcgaggcctg gacacggctg tcgttagcaa cgccccgagg gagaacgccg
     6961 ccgccgttct gcgggcgctc ggcctcgacg gcggcttcgg cctcatcgtg ctcgccgacg
     7021 acgtggggat gggaaaaccg gatcccgccc cgtaccgcgc ggccgtcgag aggctgggca
     7081 tcgccccgtc gcgggctctc gccttcgagg actcggtttc cggcatagct tcggccgtcg
     7141 gagccggtat cccgaccgtc ggcatagcct ttacgaacga cgccgccgcg ctccgcgagg
     7201 ccggcgcttt cgaggtggta gaggacttca cggacccgcg actgcgaggg ttgctggacc
     7261 gctagagtct tcctggggcg cggtacggtc gaggaaggcc gtgagcttct tcctgtagtc
     7321 ggggttcgtg tacgactgga cgtggtcgta gccttcgagg cgccagagct cggcgcgcgg
     7381 gtcggcccgg aggaagatac gggcgtgctc gtaggcgacc gtttcgtcgg tggtggagtg
     7441 gatgacgagg agcggcacgt cctgtctgcg gagctgcgcc gcctcgttcg ccgggaccac
     7501 ggaccaggga tcgaagccca gaaagagctt cgccgagagc atgatgccgg ggttgaagag
     7561 cgagggaaga ccgctattct ccggcagctc cttgcccaac aggagcggga ggtccgcgta
     7621 gcccgagtcc tcgacgactg cggcgacccc cgttccgggc gcggcgcgca cgaccgtcgc
     7681 cgcgcccatg gaccagccgt gcaggacgac ctcgtgcggc ccgtagcctc tctgttcgag
     7741 ccacgagagg gcggcgcgca cgtcgcgcac ctcgcggtag cccagggtgc ggcgctcgcc
     7801 gccggaggcg ccgtggccgc gcaggtccag catcaggacc gagtaccctt ttgaggcgta
     7861 gatgcgggcg gacttcagga tgtgctcctc ggagcggttc ccgccgaagc cgtggacgag
     7921 tattgctgcg cgttctgcgt cccgcttctc gacccaccag gcccggagcg gcacgccgtc
     7981 ctcgctctcg aacccgacgt ctctgtagcg cagccccacg tcggcgggcg tgccttcctc
     8041 gggggagcgt tcgggcgcgc tgagacgcag cgccacgagc agcccgacgc ccatgtaggc
     8101 ggcgacgagc agcgcggccc cgaccagagt tgccaggacc gctcgccgca ccgtcggctc
     8161 ctagaccctg acctccatcg tcaccgcgtt ggcagtctcc ggcagcggca cgacgatgcc
     8221 gccctcgtcc cggcgcggct cccgctcctc gccgtccacc aggacgctct gcaccgcatc
     8281 caacccgcgt agctctagca gaagatcccg aggttccggc tcgaacgagc cctcgcgctc
     8341 cccgacgcgg acggtgacgc tctgggcgtt cgtttcgcag gtaacgctgc ggcgggcgta
     8401 atcgccgctc tggtagccga agccgttgcc cgcgtcctcg tagagcgcgg actcgccgga
     8461 gccctcggcc ggatagagga ggaaggagag cgtgtccggc ttctcgtccg tgtggctgac
     8521 ctccggtccc atcggcacgg cgctgttggc cttcacgtag agcgcgggct cgccgacggg
     8581 cgcgtgcgcc aggatgtgct gcgggccgtc gaagcgttcg ccggtccagt agtggaacca
     8641 ggtcccttcg ggcaggtaga cgtggcggtg ctcgatgccg gggcgtgtga tcggggcgac
     8701 gagcagggat gggccgagca ggaactcgtc gtcggccgtg taggtcgtct cgtcctccgg
     8761 gtactcgaag aggaggggac gcaggatcgg ggctcctgtc cggtggcact cctcgaagag
     8821 tgtgtagagg taggggagga gccgcatcct gagcttgatc atcttccggc acacggactc
     8881 atacggctca ccgaaagacc acggctcctg gttgatcgtg cccttcgccg agtggttgcg
     8941 gcagaatggc tggaagaccc cgaattcggt gaagcgggcg agcagttcgc cgttggagtc
     9001 gccgaagaag ccgccgatgt ccacgccggc ccagcccacg ccggagaggc cgaggttctg
     9061 tagctggggc atgctcatcc acaggtgctc ccaccatgag gagttgtccc cggtccattg
     9121 catcgcgtgc cgctgcaggc cggcgtaacc ggcgcgggtg atgacgaagg ggcgttcgtc
     9181 gggacgcagt ttcagcaggc cctcgcgggc tgagcgggcc atcagagagc cgtaggtgtt
     9241 gtggatctgg gcgtgccact tcgggtcgcc gcctccggga tgcaccacgt tacccggcat
     9301 cgtggactgt agcggcacga agaggctcgg ctcgttcatg tcgcaccaga tccccgcgat
     9361 gcccacgtcc gtgagcgccc ggtggttctc gccccaccac tcgcgcgtct cggggttggt
     9421 gaagtccggg aaggcgcaca tccccggcca gacgacgttg tggaactcct cgccggcctg
     9481 ggtcttgcag tacaggtccc gcgcccggcc ctcgacgtag acggggtagt cctcgtcgat
     9541 cttcacgccg gggtccacga tggtgacgac gcggaagccc tgctcgcgca ggtcggcgag
     9601 gagtccctcc gggtccggga aggcgtccct gtcccaggtg aagacgcggt atccgtccat
     9661 gtagtctatg tccaggtaga tcacgtcgca cggtatgtcc cggtcgcgga actcgcgcgc
     9721 cagctcccgg atctcgtcag ccgtctcata gctccagcgg cactgctggt tccctaaagc
     9781 ccacatcggc ggcatcgggg tgcggccggt tagctcggtg tagcggtcga ggacctgctt
     9841 cggcgtcggg ccggcgaagg cgtagtacac gatgtctccg ccctcggcgc cgtagtaggc
     9901 gcggtccgcg ctctctttcg cgaggtcgaa ctcgacccgg tgcgtgttgt cgaagaagag
     9961 gccgcgggcc ctgccatcta ttatcgagag ggtgaagggg attgaggtgt agaggttctc
    10021 gaaggaggca gtgtgcccgg cgggcgggtc cacgttccag aagacctggt aggagccggt
    10081 cttctccagg ccgctggtcc gctccccgca cccgaaatac cgttctcctt cctggcgctt
    10141 cttgtacagc cgcgccggtt ctccgagcgg ggagctgaag acgtccgcgc ccggctgcgg
    10201 gacgaagccc atgccgaggt ccgggtcgtc ggcggcgaac tcattaccct ccgcgtcggt
    10261 gaagtggatg cggagcgggt gcagcgagat gtgcgccgtg gccgcggggg tcttcagcgc
    10321 gatctcttcg tccgactgct ccatcgccac ctcgaccgtc ccccagtcgt ctctggctat
    10381 ggcctcggtg tcatagcgcg gcgttcggcc gtgcggaaat gctcctaccc ggaacaggtc
    10441 cggggcgagc gccgtgatct cgatggtcgt cgagccggcc ttcaggcgca ggccgcggtc
    10501 tccatgatcc atcaactccg ccgagccgag aggctcgtag tcgccaccgg tctcgggcgg
    10561 cttttcgtag gtctcggtca tatatctcct ccctgttgag agctagcggt ttataggtat
    10621 ttttcggcca gccagttagt agctatcgag gctccggcgc ctgccttacc ttgcgccgac
    10681 gctacatctt acttgcctct cacggctccg gcgagcagga cgatggcggc gctcaggccg
    10741 acgactatgt agagtgcccc cccgagccct acaagctcgg cgacgaagcc gatggtgggc
    10801 gggccgatga ggaacccggt atagcccgcc gtggagactg cggcgagcgc gggaccggcc
    10861 gccataccct tcgtgcgtcc ggcggcgctc agggcggtgg ggaagatgac ggagaacccg
    10921 gcccccgcgc aggcgaaacc gacgagggcg aagtacggtt gccccacggc cagcgcgacg
    10981 ccgaggccga cggcggccag cgctccgcat gcccgtacga gcgccgccgg tcccaggcgt
    11041 tgggtcagcc tgtctcccag gaggcgcccg aaggccatcg ccagggagaa ggcggcgtat
    11101 ccggcggcgg cgaatccggg gccggtcgtc agcgtatcct tgaggtacac ggcgctccag
    11161 tcggccatcg cgccctcgcc gagcaggacg cagaacgaga tgacgccgag cccgagcagc
    11221 gcccgcgtgg gacgggcgaa ggccggcgca ccgtccccgg cggcgtcagc gctcgcgggg
    11281 aggagcggcc ggtaggcgac gagagcggtg ctcgcgcaga gaacggcgac cgccgagagg
    11341 tggggcaggg gaccgatgcc cgccgcggcc accagcccgc cgctcgccgc gccggcgagt
    11401 cccccgaggc tgaacgcggc gtggaaagag gacatgatcg gtcttccgta ctccttctcg
    11461 accgcgacgg cctgagagtt catggagacg tccagggctc cgttcgacgc gcccagcagc
    11521 acgagcgccg gcacgaggaa gaagaggtta ggcgcgagcg cgggaggtat gagggagaaa
    11581 gagatcaaga gcgccgtaac cccgaccacc cgccggctcc cgaacctgga agtaagcgcc
    11641 cccgccagcg gcatcgcgac catcgctccg accgccgccc cgagcagcgc cacccccagc
    11701 aggccctcgc tcagacccag gttgtcccgt accgccggta tccgtacgaa ccagctcgcc
    11761 accgccagcc cgttcaggaa gaacacggcg aggaccgaga gccgcgccgc gcgaatgcct
    11821 gtcccgttca agccgccgtc ctgtcgcaag cgccgcaggc gcccgcgtct ctgttcggct
    11881 ggccccggaa ccgcgttacc tcggcgtcga tccactccac accgtggtag tgcgccacgg
    11941 agacgcggtg gttgccatcg tggacgtagt agtcgccgcc gatctggtac aggctcaccg
    12001 gcgccaggtc cacgccactc tggaatgccc gatccaccct cttccacctc tcggcgctcg
    12061 cactcgtggg catgaaatcc cggtcgaact ccctgtgccg gcccacgctc cccacgatcc
    12121 tggacacctc gaccaccctc ctgcccagat agacccggtt gtccgcccgc gccgaccgcc
    12181 ggctctcgtc aaagagcatt attccttccc tcgcaacgtc cccgcgcagc cgcgctctga
    12241 tccatgctac gagcgaacgg cgccgcgcgc gctcgaagtc cctgtctacc tgctcctcta
    12301 aactcatgta gccgaccatc agctacctct ttctgaaacg ttttacttaa tcgtttcaga
    12361 aagagtatca tggcggctat atgggtgtca aatctgatgg gcgtgaggcc aggcggcggg
    12421 cgacgttgaa ggaggtcgcc tcggagttga gcgtgtcgcc cgcgacggtc tccaatgcgt
    12481 acaaccgtcc ggatcagctc tcggcggagt tgcgggagcg ggtcttcgag acggcccgga
    12541 ggatgggtta cacggggccg gaccctgcgg cgcgcagcct gcggcgccgg cgagctggag
    12601 cggtcggggt gctgtacacg gaccgtctct cctacgcctt cgcggacccg gtatcggtca
    12661 tgtttctgga aggcatagcg acggccaccg agggggcggg tctcgggatg ctgctcatcc
    12721 ctggcggggc gttgagggag cgggacccgg aggccatcgg gaacgcggta gtggatgggt
    12781 ttgtgatcta ctgcatggcc gagaacgatc ctctggcgac cgccgctctg gaccgccagt
    12841 tgccgacggt gctggtggac cagccggaga tcggcggcat tccgtccgtc ggcattgacg
    12901 acgaaggcgc cgcccgagcc gccgccgcgc accttatcga gctgggtcac agacacctcg
    12961 gcgtggtctc cttcgagctc gcgttaaacg cccccggcgg catcgcggac cggcgccgcc
    13021 agagcgaggc cacctaccgg ccgagtcgct cccggctcaa gggctacgct tcggccgtcg
    13081 aggatgcgga ccgatcctgg gatgaagtcc ccgtctatga gtgcacggag aacgcgccgt
    13141 cgcagggcag gatcgccgcg gagtacctgc tgtcgcggga gccgcggccc acggcgctgc
    13201 tctgcctgag cgaccaactc gcggtcggtg cctacgaggc cgcgcgcgag atgggactgt
    13261 ccgtgccgga ggacctctcc gtcgtcggat tcgacgacat ccccgccgcc gcccggatgg
    13321 acccgccgct caccaccgtg tatcagccgc acgtcgagaa aggcgtccgg gcgggagagc
    13381 tgctcatctc ccagctaagg ggcgaagagt cgtcggtttc ggagctgttg ccggcgcggc
    13441 tggtcgtgcg cggctccacc gcgccgccga gcaagtccta gaactctatg tcccggccca
    13501 tgtcccgctc catcgcccgg tcgtaggcga tgcgggccgc ggccatgtcc tccagagcga
    13561 ggccctggct ggtgaagaaa gttatatcct cggggccgcg gcgtcccggt acgcggccgg
    13621 ctataacctg tcccagctca tgcacggcct ccgggaggat cagccccgtc tccagcggct
    13681 gcatgaggtc tccggcttct agaccgatct cctctcggct atctacgcag acgaagctcg
    13741 cgcgttttat gacctcgcgg tctacttcgc tcttgaatag catgttcgac ccggcggcgt
    13801 tgacgtgcgt tccgggacgc agccactcac cgtacaggac cggctccttc gacgacgtga
    13861 cggtgacgac gatgtcctgg gcggcgggct cttcgggtga gtgggtggtc tctatctcca
    13921 tcccgagtct ctcgcccatc ttctccgcga aagacttgcg cttctcctcg gagcggctgt
    13981 agacgatgac ccgttcgagg tcgcgcacgg aggcgatggc ctccagctgg ctctccgcct
    14041 gccatcccgc gccgtagatc cctagagtgg aggcgtcctc acgggcgaga tacttcgtcg
    14101 ccacaccggt cgccgcgccg gtgcggagct ggcccatctt gtccgactgg atgatcgcca
    14161 ggaggtctcc atcatcggga tcgaagagca tggtgtagaa gcgggctccg gtacgggcga
    14221 cggtgtacgc cttcagtccc agagccccga tctccggcgc tcccgcgaac atcacgttca
    14281 ggccggcggt cgggagagcg acgcggcggc gggcgcggtt ggtggcgcgg ccctcggcgt
    14341 gctggcgcag tatctcttcg acggcgtcca gcacggacgg catgtcgagc agctcttcga
    14401 cttccagttc ggtgagtagc agtgtcacgg cgtctcctct ctgttagagc gtggtatatc
    14461 cgttaaaatc cacccactca tgcgggtcct cgtcatagac aactacgact cttttaccta
    14521 caacctcgtc cagtacctcg gcgagctcgg ggccgaggtc atcgtgcggc gcaacgacga
    14581 gctgacgccc aaagaagtcc tcgacctcca cccggacagg atagtcgtct cgcccggccc
    14641 gtgcacgccg aacgaggcgg ggatctcggt tgaccttatc tcggagtccg ccgccgcgga
    14701 ggtgccgctg ctcggcgtct gtctggggca ccagtccatc gggcaggctt tcggggcgaa
    14761 ggtcgtgcgc ggcgagcccg tccacgggaa gaccgcgagg atcctgcacg acggcgaggg
    14821 cgtctatgcc ggggtcgagc agggcttcga ggctacccgc taccactcgc tcgtcatcga
    14881 gcccgagtcg ctgccggagt gtctcgtcgt gaccagccgc acggaggacg gcgtgatcat
    14941 gggcgtcagg catcgcgagt atccggtcga gggcgtgcag ttccacccgg agagcatcat
    15001 gacgcgcagc ggccacgacc tcctgaagaa cttcctggat ggctgaggtt ccggtgctgc
    15061 gcgagtcgtt acgcaaggtc gcggcgggcg aggcgctctc gcagggggag gccgagcggg
    15121 ccctggagac catcatggag gggacagcgc ccccggcggc gacggcggcg ctgcttacgg
    15181 cgatgcggat acggggcgag tcggtggggg agatcgtggg tttcgcccgc gcgatgcggc
    15241 gtttcgccgc gaaggtcgag gcgccggagg acgtggttga cacctgcggc accggaggcg
    15301 atgcgaaggg gaccataaac atctccaccg cggcggcgtt cgtcgctcgc ggagccgggg
    15361 tcaccatagc caagcacggc aaccgcgccg cgactagcct ggccgggagc gcggacgtgc
    15421 tggaagcgct gggggcggag atagagctgg ggcccgagca ggtctcgcgc tgcatcgccg
    15481 aggccgggat cggcttcatg ttcgcccgca cccaccatcc ggcgatgagg ttcgtggccc
    15541 cggtgcgcgc cgagctgccg ttccgcaccg tcttcaacct gctcggcccg ctcaccaatc
    15601 cggccggggc gaagcggcag ttgatcgggg tcttctccgg cgactacgtg cggcccgtcg
    15661 cggaggcgtt aaaggaactg ggggcggaga aagctctcgt cgtccacggc gcggacggga
    15721 tggacgagat cacggtgagc ggtcccacca gggtcgccga ggtctcggac ggggagatcg
    15781 aggagtacga cgtcacaccg gaggacttcg gcctcccgcg ccacgccccc gacggcatac
    15841 tcggcggcga cgcccacctg aacgcccgca tcctgcgcga catcctctcc ggcgaggagc
    15901 cgggagcccc acgcgacgta gtgctcatga acgctggcgc cgccgtgttc gtctccggcg
    15961 gggccgggac gctggatgag ggcgtcaggc tggcggagcg gtccgtagac tccggcgcgg
    16021 ccctcgaagc gctggagacg ttcgtgaaga tcacccgccg tctcgccgcg ggccccggag
    16081 ggacaagatg agcatcccaa ccgtcctcga cgacctcgca gccgccgccc gccgccgcgc
    16141 cgacgaacta aagacgaaca ccgacctcga tgcgctctac gagcgagcca tacgcttcga
    16201 gaagcgcgac ttcgcggccg cgctcaggtc tcccggcctc tccgtgatct ccgagataaa
    16261 gcgggcctct ccgtcggcgg gtttcatccg cgacgccgac cccgccgagt ggggcgcgcg
    16321 ctacgagagc gagggcgcga gctgcctctc cgtactcacc gaacctgacc gcttcaaggg
    16381 gagcctcaaa gacctcgacg ccgcgcgcga gaacaccacg ttgcccgtta tccgcaagga
    16441 tttcaccgta gacgaggtac atgtcatcga agccgccacc cgcgccgacg ccgtcctcct
    16501 catcgcggcc ctcttcgacg cgacctcgct ctcgcgctac gtagcgctct ccatcgaggt
    16561 cggcctgacg ccgctcgtcg aggtccacga cgagcgtgag gcggagatcg cgctggagtg
    16621 cggcgcgacg gtgatcgggg taaacaaccg cgacctgcgc gacttcaccg tggacctcgg
    16681 gaccttcgag cgcctcgctc cagggcttga tggagccacg ctcgtcgccg agagcggcgt
    16741 aaagacgccg gaggacgctg gacgattgcg ggatgcggga gccgacgcgg tcctcgtcgg
    16801 cgaggcggcg atgcgtgacc cgtcgctcgt ggcgaagctc tccgcactgc cctgacctcc
    16861 atcggccggg tctatccctg gatctcagat ggagcctttc tcgttcgtgg ctatgaagag
    16921 tgaggccgta tccgagaggt gactttacgg aaaatccatc tggccctcga aattacctgc
    16981 agttacaatt tggatagggc ttttttaagg agcgccatgg acgacaggac agccgtgacc
    17041 caggtactca ctgactatta caatgctttc ggcacacttg acgttcaaag aattttgtcc
    17101 tactaccatg agccctgcat gcttgtcagc ccccagggag tcgccgcgct gcccagccgc
    17161 gccgcaatgg cggctgcttt tacgcccatt atggaaggct tgcgggcaag aggatacagt
    17221 cggagtgagt cgagcatgct gcacgtcaag gagctcagcg ccactgcggc gctggcaagc
    17281 ggcattgccg tacgctacag ggccgacgga caggaattgg agcggatagg cgtcagctat
    17341 cttctgcaca agactgacga ccgttggaag atcgcggtaa ctgtggttca cgatacggat
    17401 aatgttgtga gatcagaata ggcggcgaca ggcccactat catctagagt cctcgaattc
    17461 tgaggggtat tcgcgcagta cgagcaggcc tgcggcaagg cctgcggcag cggactcttg
    17521 gtggaagcca tcgatgtgcg atcccttata ccaactcgct tacaaatcgt ggcgatgaaa
    17581 aaacgtccat gaaacacccg gcttgatgtt tcgatatatg ctggtagggt atatgtgcag
    17641 caagaacata gatgacctac tactacggag gtatcgcaat gacgactacc gcaaacatca
    17701 acaagatgac gcgcaccact cgggagatag ccgaggtaca gcgcgactcc tttgaagctc
    17761 tggcggagaa cttctccgat ctgcagcggc gcaatatcga gttcgcccag ggtggattcg
    17821 agttcataag gcttcaagag gacaacgcca aggctgcgcg cgagtggttc gccaacggcc
    17881 tcaagttggc gaagctgcag cagcgcaacg tcgagttcac ccagaagtgg atgagcggcg
    17941 gggtcgagtt gctgcgggat caggccgagc acaaccgacg cgcggtggag gtcttctcgc
    18001 agagcacgcg caagcagcag gagaacttcc gggcgctcgc cgaggaatgg accggcgctt
    18061 accggaactt cttctctcct ttcgcctacg cgcaggaagg tctgaaggct gctcagcaga
    18121 ccactcagca ggcagtgcag gccacgcagc aggttgctca gcagggactg cgggtagccg
    18181 aggagacgac caggaagagc gagcaggcta tccgcaagac ccaggaggcg acccgggagg
    18241 ctgctctgca gaccgctgtt cacagcgcgc tgaagaccgc gaactacgaa gatctgaacg
    18301 ttgacgaggt ctcgaagaag ctcgacggtc tctccgccgc tgaactcaag aaggttcgcg
    18361 agtacgagaa gaagaacaag aaccgcgaga ctctcatcga gcagatcgac cgcaagttga
    18421 aggctacttc gtaaggcact tcttcacaaa aagtagtttc aagggccggg gcaacagtcc
    18481 cggcccttct tcatgccttt tccggaaaat tccgcataca ttaactctgg gaattagata
    18541 tacagttaca ctacgatagc cttcagacgc tacagaggag agggctcacg ccgttgatcg
    18601 aaaggtctcc atgtttgcgg acctcttcct cgcggggatc tctgatcggc acatttatga
    18661 ggccacctac ccggaaggcg tttaggactg cgctcatcgc gtaatcggga acctcggcgc
    18721 cctctctcag aagacgctgg taaacgacgg cgctctccac ggagccgcct tctccggacc
    18781 actcatcgca gatgatctct ctcactttct cggctagcgt tcgaccggga tctcctttgg
    18841 ccactcaaag ctccttctct cgacctgtcc gaggtattag tgtactagcc gacacctgtt
    18901 gcgattgacg gtggaccaga ggatgagagt atctggcggg ctcaggagcc gaatcggttc
    18961 agtggaggtc cctctcagcg agtaactcct ggatcttctt ctccgtctca tcgtaggcgc
    19021 cctctccgat gtgcttgtat ctgacgaagc cgtcggcgtc tatgaggtac cacgtcggcc
    19081 agtaacgctg gctgtaagcg ttccaggtgg cgtcgttgtt gtcgagggcg acggggtact
    19141 ggatgttggc gtctttcaca gcctccgaga cgctggcgta gtccttctcg aactcgaact
    19201 cgggtttgtg gatgccgatt acttgcaagc cctcaccgtc gtacctttcg tagaagcggt
    19261 tgatgtgcgg ctgcgcgttc tggcagttgt agcaactata ggtccagaac tctagcagga
    19321 cgaccttacc gcgggcatcg gccagcgaga tcccatcggt gttgataaag ccggtcgggt
    19381 ccactatctc gtgcgcgcgg ggatacctcg cttgtttctg agcgatgctc tctccccgga
    19441 tgggcgattc gtctttggac cgttcctgct ccgcggagtc ttccttaccc tgttcgggaa
    19501 ccgttcggag ccccgcagtc cgggacgggc cgttcgcctc cgtgtttgaa ggttttacgg
    19561 aggcagattg cgtccgctga tcgctcgcat ccgccgcttt gaatcggatc tggacaaaag
    19621 agatcgcgcc ggctatcagg atgagcgtca gagttagaag aacagggtgg gggacgatgg
    19681 acctcatagc aatacctcat ttagtagctg aaagttggcg agcagactga gcttgtccgt
    19741 gaacacgagg actcccatcc cgatgaggac gccgccgaat actgtatgga gccgcgcagc
    19801 ccacgagcga tgcttcctga tgagcgcgaa cgctccggag ggaaagagtc cgacgagcag
    19861 gaacggcgtc ccgagaccgg tagcgtaggc gagaagtagc gggaacgccg ctgcgggctc
    19921 cgaggccgcg agtgcgaggg tgctgccgag gatggggcca acacagggcg tccaggccac
    19981 cgcgaaggat gctccgaaca gcagagaggt caggtagccg ggtctggagt taccggggtc
    20041 taccgaatat tgccggtcca ggatgggaag tctcagcagg cccgtcaggt gcaaccccag
    20101 gacgatgatg accgctccag cggtgcgcga gagccaggca aggacttccg cggccccctg
    20161 ttgaagcacc gcgttgagga ctataccgag tagcgcgaac accgtcgaga acccggctac
    20221 gaagagggcg gtgctcagca ggaggtaccg gcgcttcggg ctcgtgacgc ccagagaggc
    20281 gcccgccagt tgggcgagga aagcgggaat cacgggcagc atgcacggag agaggaaaga
    20341 cgccaatccc gcggcgagag ccagtacgag cgtcggctgc gtcatcgctt caggctttcg
    20401 gggtcgtttt cttcacgcgg ccgatctcct ggggtagctg ccggtccttc ggcgattctg
    20461 gcacgcggtt ttgagagcga caacgctttt gggcgcccgt taaccctatc gtaaggaacg
    20521 gttaaggttt cggcgggatc aggcgcggcg cagggtgaac cggaagcgcg agccgcctcc
    20581 gggatggctc tcgacctcga tggcgccgcc gtgggcttcg atgaggccgc gggctatggc
    20641 gaggccgagc ccggcgttcg aatcgccgtc atcgccggag cgggtccggg atttctctcc
    20701 ccggaaggtg cgctcgaaga cgcgcggcag gtcttcgggc gctatgccct cgcccgtatc
    20761 ggcgacctcg acctgtacct cggagccgat gggtttggcg cgcagggcga tggtgccgtc
    20821 ggagggggtg tggcgcaggg cgttggagac gaggttgtgc agcacgcgct ggaggcgggg
    20881 cgggttgatc aggaccgggt ccacgccctc ggagacctca cccacgagtc ggacgttctg
    20941 gcgctcggcc tgcggctgga agctggagag ggtgtcggag atcatgtcgt gcagcgaccc
    21001 gccttcgagc tccagcttga gcacgccggc atctatcctg gagagctcga agaggtcgtc
    21061 cacgagctgg ctgaggtgca tgagctcccg gcgggcggat tccaggtagc gggcttcggt
    21121 ctccgggccg gcggccacgc cgtccaccag ggcttcgacg agggcgcggg tcgcggcgag
    21181 cggcgtccgc aggtcgtggg agatggcggc gatgagttcg cgccgggcct tctccatctc
    21241 gcgctctcgc tcggcggact cttttagaga ccgggccatg ctgttgaagt cgcgggcgag
    21301 ggcggtcaac tcgtcgtggc cctcggcggg tatctccgta tccaggtctc cgttcgtgag
    21361 tcgcgcggcg cccaggcgga cgcgctcgat gcggtttgcc agcggcgcgg cgaagaggag
    21421 gctgaatcca acggagagga tcgcggcgaa gagcagcatc gtcaacacga ccgcgaagtc
    21481 gtggtcggag atgaacatcg cccgcgctcc cgccagcatc atccccagga ggagcaggct
    21541 cccgatcagg cccgccccca ctagctgcgc ccgcacgccg cccaacctcc cgagcaccgc
    21601 gggccgcacc aggagcagcg ccacgagccc ggcggcgccg cccgccccga ctagcaggag
    21661 cgcgatgtcg ggcacgtccg ccgccggtat gccgaacaac gcggcgctca ccaggaccgt
    21721 taccgccagc cccgcgaaga ggaccagaag ccccgcgagc agcctcttga tcatcgttct
    21781 caggctcgcc cgaatttgta tccgacgccc cagaccgtct ggaggtagcg cgggtgggtt
    21841 gggtcggcct cgatcttctc gcgcaggcgc ctgacgtgta cggtcacggt gctggtctcg
    21901 gcggcgaagg cgtatcccca gaccgtctcc atcagatggt cgcgggtata gaccctaccg
    21961 ggactcgatg ccaggtagta gagcaggtcg aactctcggg ccgtgagcgt caccggctcg
    22021 tcccggacta acacctcgcg cctgttcggg tctacgcgaa ggccctcgaa agagagcacc
    22081 ttctgacccg gcttccggcc cgaatcaggg ccggattcct ccacgcggcg cagcacggcg
    22141 gctacgcgcg cggcgagctc gcggggcgag aacggtttga ccacgtagtc gtccgccccc
    22201 aggctgaggc cgactattcg ctcctcttcc tcgccacggg cggtcagcat gatcagtggc
    22261 acgcgccgct cggcctggat gcggcggcat acctcaagac cgttgatcct gggtagcatc
    22321 aggtccagca cgacgaggtc cggctcgctc ctgcggaaga cttccagagc ctccacgccg
    22381 tccgccgcgg agacgaccgt gtggccgtct cggctcaggt agagcccgac aacctcccgg
    22441 atgttcggct catcatcgac aacgagaacc ttggccacca catccctcgt ctctccgcta
    22501 agtccctcga ttataatgcg ccgactctgg ccacggggct ccgggcatgg cgagcaggcg
    22561 agtcagcatc ccgacactat ccatcttcag cgctccaatc gcatcaacgc cgctccaggt
    22621 cgagcgccag gctgttcatg caccaccgct tcccaccggc gtcccgcggc ccgtcgtcga
    22681 agacgtggcc gagatgcgag tggcagcggg cgcagcggac ctccgtgcgt accatcccat
    22741 gagagtggtc ctccaccagc tcgacggcgt ccggcgagac cgcttcggtg aagctgggcc
    22801 agccggtccc cgaatggtac ttggtgcggc cgtcgaagag cggatttccg caggcggcgc
    22861 agtggtagag tccatcgtcg tcggtgtcca cgtactcgcc ggagaacggc cgctcggtac
    22921 ccgcctcgcg cagcacggcg tactgttcgg gcgagagtcg ctcccgccaa tcctcgtcgc
    22981 tgctcgctac cggcttgagt gcaccattgg gctcggctat cgttcccacg cccagcccga
    23041 ccgggcagct tacgccggta ccgccgaggc cgcagtaccc gttagggact ttgtggaggt
    23101 actgctggtg gtagtcctcg gcgtagtaga acgggccggt gggcgctatc tccgtggcga
    23161 tcgggccgta gcccgcctcc ttcagtttct ttccgtaggc ggcgcgagtc ttctcggcgg
    23221 ccccgcgttg ctcgtcgttc gcgtagtaga tcgcggaccg gtactgggtg ccgacgtcgt
    23281 tgccctggcg catctcctgt gtcgggtcgt gagcttccca gaacacggct agcagctcgc
    23341 gataggtgac tttccgggga tcgaagacca ccagcacggc ctcggtgtgc ccggtccggc
    23401 cgccgcacac ctctccgtac gtcgggttcg gcgtatagcc gcccgcgtag ccgacagccg
    23461 tggtgtagac gccctcggtc ttccagaaga cgcgctccgc gccccagaag cagccgaggc
    23521 cgaagaccgc tgtttcgaga ccctcgggga acggcggctt gatcggcgtg ccgaggacgg
    23581 cgtgcctctc ggggacggcg aacgctggct gatcacgccc agggagcgca tcctcgggcg
    23641 ggaccatcgt ggacttgtgg cggttgaaca ggaacctcat gtttactcct tatctggtcg
    23701 ttctgtcctc ttgcacgttg cgggcggtcg ggccggtgtt ggcgtgttcg accacctcga
    23761 acctcaccgg cgtgccgggc cggatggtgc ggtagccctc gccggggatg gcggtgaagt
    23821 tgaagaacac gtcctcgtcg ccgtcgcgcg agatctcacc actccccgac gcaaggtcga
    23881 aggtctttac ccggccgctc gccaccggca gcccgttcgc tcccagaggc ggcgcgccgt
    23941 ccgcccactc ctgctgcggg atgcggatac tcttagcctc cggggtgtcg aacgcctcgt
    24001 aaaggtagtc tctgcggcgg gacatgatct cgcgcaggtc gtgccttagc tcctctgcgg
    24061 tgggccgtcc gacgaagaac cagccgtcgt agaccttgtg gaccgtcagg tcaggccgca
    24121 ggacgaacgt gaacggccgc gcccggtagg cgtactcgcc ctcggtctcg tccagaatgc
    24181 cgatgccctt gatgacctcc cgatcctcat ccgagaggaa cggccactcc gcccccagac
    24241 ccacccggaa cgccgcgcca accatcggcg ggtcggcgct gaccgtgacg agcttgcagt
    24301 agttgaccgc aagctccgac tggaagcgga caagtacccg catctgttcc tgatcccgcg
    24361 ggcagaagaa gccccggccg aagaccacga taaccggata accgtccgtg aagcccagcc
    24421 tctcatccat cgcgctcggg tgggtgaacc gggagagccg tcgcggtacc ttgcggtgat
    24481 ccggcagttc gaagtcgggg aaggcgtctc cgacacggag ggtggtcatc tcatcactcc
    24541 acgaagctgg cgaacaggtt gtttagcgac tcggcgaggg tggggtgggc gaaggcgccg
    24601 tcgcgcaggg cggtgtatgg gaccttaccg atcatcgcga cgtggatgag ggacatgatc
    24661 tcgccgccct cgatcccgag taccgcgcaa ccgaggatcc ggtccgtctc cgcgtccacc
    24721 atggctttca tgaagcctcg cgtctcgtcc atctccagcg cccgcgccac gcggctcata
    24781 ggcatcttcg ccacccggaa gtcgaggccc tgctcccggg cctccgtctc gttcaagccc
    24841 acccgcccga gctgcgggtc tataaagacg gtataggaga ctatccggtc cgcgatggag
    24901 gcgttcccgc cgtcgagcag gttggtcttg aggacgcgaa agtcgtcgta ggagatgtgg
    24961 gtgaacgccg gacctccctt tacgtcgccc agggcgtaga ctcccggagc tgtggtctcc
    25021 agcctgtcgt tcacctggat aaagccgcgt ccgtccgtct cgatgccgcc cgcccgcggg
    25081 tcgagcgagt ccgtgttcgg cgggcgtccg atggctacca gcaggtgaga gccctccagt
    25141 acgcgctcac cgccctcgtt cttgacggtg agttcgatgc cgccgctgcc gttctccacg
    25201 cgctgggctt cggtctggag caggacctcg atgccgtctt cgcgcaggac gtcggcgacg
    25261 gcctcggcca cgtcggcgtc ctcgcgggcg aggagttgct tcccgcgctg cacgaccgtg
    25321 acctcgctgc cgaagcgccg gaacatctgg gcgaactcca tgccgatgta gccgccgccg
    25381 aggaccagca ggtgatccgg gacctcgccc agctccatga tcgaggtgga gtccaggaac
    25441 gggacgttgt ccagaccgtc tatcggggct ccgccgggcc gagccccgac gttgatgaag
    25501 acctcgtcgg ccgtcaccgt gagcgtaccg acgccgttcg tgcggatctc cagttccttc
    25561 gaggcggtaa agcgagcctc gccaacaagc aggtccaggt tctcggtact ctcgatgccc
    25621 ttctcgccgc cgctccggaa gcgctcgacg atatcgcgct tgcgctggcg gaccttcgtc
    25681 atgtccacgg caaccggccc gtcctggacg ccgtagtcgg gcgagcggcg gtccaggtag
    25741 gcgacgcggg cgctggcgac catcgtcttg gtcggggtgc agccgacatt cacgcaggtg
    25801 ccgccgacgt gttcgcgctc gatgatcgcc gtccggcgtc cggccccggc gagagccttc
    25861 gccagcggtg tgccgccctg gccggaaccg acgatgacgg cgtcgtaatg ctgggtctcg
    25921 gtcatgatct ctccttgtcc tgttagccgt gttcgaggcg ttctctggtg gttttatacg
    25981 gagcccgcgg ggccccgggt gcgtgaaggc ttacatttgc ccgtttgcca gccagtccag
    26041 gagcacggcc gcggtccagg actggcggtc ggagcccaga ggctcgccgg tgaagggctc
    26101 atagtattcg ctgaaggtcc catccgcgag ttgcgcgaga ccgtcgcgcc tgagccgggc
    26161 ggcgcgctcg tcctcgccgg agcgctccag cgaccaccac aggagccagt tgatgaccgg
    26221 ccagacggga ccgcgccagt agcggcgggg atcgaagagc ggctcctccg ggctggtgct
    26281 cggcgggagc ggccagcgga gcttcgagtg tcccgcgaag agcgctgagt ccagagtctg
    26341 gaggagactt cgccgtccgg tttcgatgcc cgctataagc ggagcgaaac cggcgacggt
    26401 gcgggctttc agcggcgccc cggcgcgcag gtcgtagtcg agacagagcc cgatttcggg
    26461 gtcccagctc ttcgttagcc cgcgccggcc gcgggagatc caggccgata taacgttcct
    26521 gtcttcatcg ggcgctccga ccagctcgga gatctcaagc agcgcctcgt tggcgcggac
    26581 caggatcgcg ctcatcaaca cgtccttgac gaggaacggg ctggtggcgt agacgactgc
    26641 gtcgtcgcag ccgctgcgcg ttatgagctg cacgagccag ataaagcggt cgtagacttc
    26701 ctgcgtgggc ctctcggaag ggtcgtccac gtgttgcaga tcccggcgct cgtactcgag
    26761 cttctcccgg acctccacgc ggtcgagcgc gtcctgccag cgtggggagt tgtccgtgcc
    26821 gctctcccag ggatggtaga tcgtgacgag cccggactcc tccgggtcgc gggcggtcat
    26881 caggtaacgg tgccagaaca ggagcttcgg atagatctcg cgcaggaact cccgcgctcc
    26941 ggctgcgtca ccatcccgcc gctccgctat ctccaggata cgcctcgcgg cgatggcgtg
    27001 gaccggcggc tggcacaggc cgctggtggt ggcgggcttc gcgggggcgg cgggggcgtt
    27061 cggggcggcg gaggcccagt gctcggggcc ggggaagtag ctctcgggcg gggcgtccgg
    27121 attgaagacg atgtgcggga ccttgccggt ggcccactga tggtcgagga ggcagcgtat
    27181 ctcctcggcg gcgcgggcgg tatcgtagtg ggcgatgccg atggctatga acgccgagtc
    27241 ccagctccac tggtgcgggt agaggcttgg cgcggccttt gtccagacac ccatatcgtt
    27301 gtcgcgcagg acccgctccg cgcgcgcggc caggtctttt ggctgcaagt ctgtcttgga
    27361 gtcgtgtgtc gtatccatcc ggcgttgggc tcctttaaag gggattatct cagagggtca
    27421 gaggggccgt ggcgaagttc acggagaacc gcttacacca gtcggagatc gaacggtgcc
    27481 tctgcggggc ggacctggcg ggcaactcgt aggtccggtc gccccgtcga tccgcttgtt
    27541 gaccgccgcg tcgtagaaga gcgtctgcct gccgaagacc acgagcgaca gatagagcgc
    27601 cagcgccgca tcctccggcg gagggtatga atagcgcccg ccggaggatg cggctccgaa
    27661 ccagtaccct gttccattcc cgttcatcct gtcggatgct agcccgccgg ccttacgtaa
    27721 cccttgcgct tcccttacga aacccttaac ggcggcgcgg agccagccgg aaacggttcg
    27781 gcgggagagg ggttccggct tcccggataa ggagtacact tcgactcatg gtactggtca
    27841 aagtctgtgg gataaccaat ccggaggacg cccgcgtggc cgccgacgcc ggcgcggacg
    27901 ctatcgggat cgtcttcgcc ccgagcccgc gccgggtgag cgtggaagat gcgcggaagg
    27961 tggccgggac tctcccggat gacgtcctga aggtcggggt gttcgtggac gcggcgccgg
    28021 aggagttgct gcgggtagcg ggagaggtcg gcctcgacta cgcgcagctt cacggggatg
    28081 agggaccgga agatgtaacg catctacgga aaaacggtct aaagataata aaggcgctgc
    28141 gggtccggga cgcggcctcg ctggaggctt tggaggagta cggggcggac ctgtacctgc
    28201 tggatgcgta cagcgagaag gcgcggggcg ggacgggtga gcggttcgac tggaggctgg
    28261 cgaaaacgct gcgggcgcgt gataacatcg tcgtctccgg ggggcttaat cccgagaacg
    28321 tgcgggaggc catcgagttc ttcgagccct acggggtcga tgcttcttcc tcgctcgaag
    28381 acaggcccgg agtaaaaaac ggcgagcggg ttcggaggtt cgtgagtgcg gcaaaaagta
    28441 gatagtgggt acttcggtcc ctacgggggc cgctacgtgc cggagaccct gatccacgcc
    28501 ctcgacgagc tggaagacgc ctacgagcgc tacaagaacg ccccggagtt cctggaggaa
    28561 ctggacgggc tcgcgcggga cttcgtggga cgtccgacgc cgctgatgtt cgccggacgc
    28621 ctgacgcgca agtggggcgg ggcgaacgtg tggttcaagc gggaggacct ggcgcatacg
    28681 ggggcgcaca agatcaacaa cgcccttgga cagatgctcc tcgccgagcg catgggcaag
    28741 acccgcataa tcgccgagac cggggcgggc cagcacggcg tcgccacggc caccgtcgcc
    28801 gccctctacg gcaaggagtg cgtcgtctac atgggcgagg aggacacccg ccgccaggcc
    28861 ccgaacgtcc tgcggatgaa gctcctcggc accgaggtcc ggcccgtcac gagcggttcg
    28921 gggaccctga aagcggcgct aaacgaggct attcgggact gggtcgcgaa cgtggaggac
    28981 acgcactaca tcatcgggac ggtggcggg
//
LOCUS       DASSMB010000178         3074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_677722, whole genome shotgun sequence.
ACCESSION   DASSMB010000178 DASSMB010000000
VERSION     DASSMB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677722"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VFJ72_10635"
     CDS             complement(<1..236)
                     /locus_tag="VFJ72_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479956.1"
                     /translation="MGQWVAAFVGGHARENYAFTRANGEFWVSSVRTPIRKARPGDRA
                     LLYMTGEGFVGEVEVVSGARAPSGEVRWTGDAALG"
     gene            324..1619
                     /locus_tag="VFJ72_10640"
     CDS             324..1619
                     /locus_tag="VFJ72_10640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET7479957.1"
                     /translation="MVLRFGTTLDKRRSVGGEMNNGRPEAYITGIGVVSPIGVGRERF
                     WSALLANESGMGPITQFDTDGFEVRIAAECTDFEPKDFMDRKVVQRTDRFAQMGLAAS
                     KLALEDASVWDVLNQRPERVGLILGSGLGGVASMEQTQATIDSKGPGRVNPFAVTKIM
                     PNSGAAHVGIQLGVQGPSSTGALACSCGTDMMGVGFDLIRRGDADVVLCGAAEATITP
                     VIVAGFAAMRAMSRQNDDPEGACRPYDIDHSGFVIGEGAAVMILESAESVERRGVEAY
                     AKITGLGRTTDAYNITDPDPEGRGLLRAMQLAVKGAGLEGEQIGYINPHGTGTVAGDG
                     PESQALARVNPNVKVSGTKSTLGHSLGAGGAIETAICALALKERTIPPMRNLTKIADG
                     CADLDYVVNEPRGVPNLEAAMCANLGVGGHNVAVVLEQA"
     gene            1628..2212
                     /locus_tag="VFJ72_10645"
     CDS             1628..2212
                     /locus_tag="VFJ72_10645"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HET7479958.1"
                     /translation="MAHPNINELPRDLPVPEDDGACDHLPRLRLPSVPLRSTMSETLD
                     LSAFEGRTVVYCYPMTGRPDRELPEGWDEIPGARGCTPQSCSFRDHHEELGTLGAQVF
                     GLSTQDTEYQREAVERLHLPFDLLSDTDLAFARALDLPTMEVEGMILIKRLTLIIDDG
                     QITKVFYPVFPPDRSAEEVIEWLSKQDDPQRTGR"
     gene            2209..3063
                     /gene="larE"
                     /locus_tag="VFJ72_10650"
     CDS             2209..3063
                     /gene="larE"
                     /locus_tag="VFJ72_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET7479959.1"
                     /translation="MKPSRADAVERRLAELEAVVAPYGSALVAFSGGVDSSLALAVAA
                     RALPKQKVLAVTSNNETYLPSELDLAAEFARSLGVEHLVVNTRELDDPNYASNPTNRC
                     YFCKSTLYSDLGELAVARGYGCVVDGANKDDEGDYRPGRKAAKELNVVSPLSVAGVTK
                     DEVRELAKYLGLPSWDKPALACLSSRFPYGQEITPEKLAQVARAEEFMRREGFQQVRV
                     RHHGEIARLEVGPEELERAFAEREKITAELKDAGFLYVTLDLAGYKSGSLNAALGKPG
                     RKSLPVIG"
BASE COUNT          565 a         1014 c         1031 g          464 t
ORIGIN      
        1 ccgagcgcag cgtctccggt ccaacgcacc tccccggatg gagcgcgggc gcccgacact
       61 acctcgacct ctccgacgaa cccttcgccg gtcatgtaca gcagcgctct gtctcccgga
      121 cgggccttgc gaatgggagt cctgaccgag gagacccaga actccccgtt ggcgcgagtg
      181 aacgcatagt tctccctggc gtgccccccg acaaatgccg ctacccattg tcccatctcg
      241 cctccccgag tcttccagct tgctcaccag aagggttatc gtcccgaaac ggtccgggct
      301 ttagttggag tcgaaaggtg gaggtggtgc taagattcgg gacgacgctg gataagagac
      361 gttctgtagg gggcgagatg aacaacggac gcccggaggc ttatataacg gggatcgggg
      421 tggtcagccc catcggggtc gggcgcgaga gattctggag cgcgctgctc gccaacgaga
      481 gcggcatggg ccccatcacg cagttcgaca ctgacgggtt cgaggtccgg atcgccgccg
      541 agtgtacgga cttcgagccg aaggacttca tggaccgcaa ggtcgtgcag cggacggacc
      601 gcttcgccca gatgggtctc gccgcctcca agctcgccct ggaggacgcc agcgtgtggg
      661 acgtgctcaa ccagcgtccc gagcgggtcg gtctgatcct gggcagcggc ctcggcggag
      721 tggcgagcat ggaacagacc caggccacca tagacagcaa gggaccggga cgggtcaacc
      781 cgttcgccgt caccaagatc atgccgaact ccggcgccgc tcacgtcggc atccagctcg
      841 gggtgcaggg accatcctcc acaggcgctc tcgcgtgctc gtgcggcacg gacatgatgg
      901 gcgttggctt tgacctgatc cgtcgcggag acgccgacgt ggtgctgtgc ggcgcggccg
      961 aggcgaccat cacccccgtc attgtggccg gtttcgccgc gatgcgggcg atgagccgcc
     1021 agaacgacga ccccgaagga gcctgccggc cctacgatat agatcactcc gggttcgtca
     1081 tcggcgaggg cgcggcggtc atgatcctgg agagcgcaga gtcggtggag cgccggggcg
     1141 tggaagccta cgccaagatc accggcctcg gccgtaccac cgacgcctac aacatcaccg
     1201 acccggaccc ggagggccgc ggcctcctgc gcgccatgca gctcgcggta aagggcgcgg
     1261 ggctggaagg ggagcagatc ggctacatca acccccacgg cacgggcacc gtcgccggag
     1321 acggcccgga gtctcaggcc cttgcccgcg tcaacccgaa cgtgaaggtc tccgggacca
     1381 agtcaaccct cggccactcg ctcggcgcgg gcggcgccat agagacggcc atctgcgccc
     1441 tcgccctcaa ggagcgaacc atccccccga tgcggaacct caccaagatc gccgacggct
     1501 gcgccgacct ggactacgtc gtgaatgaac cgcgcggcgt ccccaacctc gaagccgcca
     1561 tgtgcgccaa cctcggcgtc ggcgggcaca acgtcgccgt cgtcctcgaa caggcgtagg
     1621 ggtgcttgtg gcgcacccca acatcaacga gctgcccaga gacctgccgg tccccgagga
     1681 cgacggagcc tgcgaccacc tgcccagact gcggctaccg tccgtgcccc tgagatctac
     1741 tatgagcgag acattggacc tctcggcgtt tgagggacga acggtcgtgt actgttatcc
     1801 gatgaccgga aggccggacc gggaactgcc tgaagggtgg gacgagatcc ccggagcccg
     1861 cggatgcacc ccgcagtcct gctccttccg ggaccatcac gaggagcttg ggacgctcgg
     1921 cgcccaggtc ttcggcctga gcacccagga cacggagtac cagcgggaag cggttgagcg
     1981 gctacacctg ccgttcgacc tgctgtcgga cacggacctg gctttcgcgc gggcgctgga
     2041 cctgccgacg atggaggtcg agggcatgat cctcataaag cggctcacgc tcatcataga
     2101 cgacggacag ataaccaagg tcttctatcc ggtcttcccg ccggacagga gcgcggagga
     2161 agtgatcgaa tggctctcga aacaagacga cccacagaga acgggccgat gaagccctcc
     2221 agagcggacg ctgtggagcg gaggctcgct gaactcgaag cggtcgtcgc gccatacggc
     2281 agcgcgctgg tcgcgttctc cggcggcgtc gactcctcgc tggccctcgc cgtcgccgcg
     2341 cgggcgctcc caaaacagaa ggtgctcgcc gtcacctcga acaacgagac atatctgcct
     2401 tcggagctgg atctggcggc ggagttcgcc cgttccctgg gcgtggagca cctggtggtc
     2461 aacacccgag agctggacga cccgaactac gccagcaacc ccaccaaccg ctgctacttc
     2521 tgcaagagca cgctctactc cgacctcgga gagctggcgg tagcgcgcgg ctatgggtgc
     2581 gtcgtagacg gggcgaacaa ggacgacgag ggcgattacc gtccgggacg caaggcggcg
     2641 aaggagttaa acgtggtctc gccgctctcc gtcgcggggg tgacgaagga tgaggtgcgg
     2701 gagcttgcga agtacctggg gctgccgagt tgggacaagc ccgctctggc gtgcctgtcc
     2761 agccggttcc cttacgggca ggagatcacg ccggagaagc tcgcccaggt cgctcgggcc
     2821 gaggagttca tgcgccgcga gggtttccag caggtccggg tgcgccatca cggggagatt
     2881 gcccgcctgg aagtcggccc ggaggagctg gagcgggcct tcgccgagcg cgagaagata
     2941 accgccgagc taaaggacgc cgggttcctg tacgtcacgc tggatctcgc gggctacaag
     3001 tccggcagcc tcaacgccgc gctcggcaag cctggcagga agagcctgcc ggtgatcggc
     3061 taggtccgta gtgg
//
LOCUS       DASSMB010000179         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1310665, whole genome shotgun sequence.
ACCESSION   DASSMB010000179 DASSMB010000000
VERSION     DASSMB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310665"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1486
                     /locus_tag="VFJ72_10655"
     CDS             <1..1486
                     /locus_tag="VFJ72_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET7479960.1"
                     /translation="EARGFWIAEAVWITHAMGVARAMAEWIVSGVPSIDLATCDVHRF
                     EPHALSDDYLNARNSRSYEEVYDIIHPQQPMEEPRPLRTTPFYGRQQDLGAYFMESSG
                     WERPHWFEANEPLVEGRDIPQREEWSGRYWSPIAGAEHQVTREKVALYDMTPLKRAEV
                     TGPGALEFLQRLNTNQLDKPAGSVTYSLMLDEAGGIRSDITVARLGEETFQLGLNGPR
                     DILWMEDHLPEDGSVHVRDVTGSSCCIGVWGPLARDLVQPLTDDDLSNEAFGYFKARR
                     IYIREVPVTALRVSYVGELGWELYAPTEMGLRLWDLLWDAGQPLGVIAGGRSAFGSLR
                     LEKGYRLWGTDMSTEHDPYEAGLGFAVKPDKGDFVGREALIRRREDGLRRKLSCLVLE
                     DPNVVVVGNEPVYSGGEPVGYVTSAAYGYSIGKSIAYGWLPPELSRVGTRVEVEYFGE
                     RHGATVSAEPLFDPEMRHLGVVRKSPEPAARAASYARVPADGGV"
     gene            1598..2377
                     /locus_tag="VFJ72_10660"
     CDS             1598..2377
                     /locus_tag="VFJ72_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7479961.1"
                     /translation="MRPVDAVNKSGSVRSVNRAVAIMRFLSSHGPATLTEVSDGVEIY
                     KSTAHRLLATLRDEGLVEQDAATTRYSLGYGLALLASSVTSGPDILRCARPVCERLSE
                     ETGETVTVAVLENNEAVVIHQALSNSSALSVDWTGRHTPIHATGAGKVFLAHMPESQL
                     DSIMVDPLERFTEHTVVEPARLREDLEDIRARGYAICFEELEPGLNVIAAPVRFSQGP
                     VTAAISVSGPAFRFPPDGAEAVGELTCGAADEISRCLGFWE"
BASE COUNT          496 a          814 c          973 g          486 t
ORIGIN      
        1 ggaggcgagg gggttctgga tcgcggaggc cgtctggatc acgcacgcca tgggcgtcgc
       61 ccgcgcgatg gccgaatgga tcgtctccgg cgtcccgagc atagacctcg ccacctgcga
      121 cgtccaccgc ttcgagcccc atgccctgag cgacgattac ctgaacgccc gcaactcccg
      181 ctcctacgaa gaggtctacg acatcatcca tccccagcag ccgatggagg agccgcgccc
      241 gctccgcacg accccgttct acggccgcca gcaagacctc ggagcatact tcatggagtc
      301 ctccggctgg gagcggccac actggttcga ggcgaacgaa ccgctagtgg agggacgaga
      361 tataccgcag cgcgaggagt ggtccgggcg ctactggtcg ccaatcgccg gcgcggagca
      421 ccaggtaacc cgcgagaagg tagccctcta cgacatgacg cccctcaagc gagcggaggt
      481 cacgggaccg ggcgccctgg agttcctgca acgtctaaac accaaccagc tcgacaagcc
      541 cgccgggagc gtaacctact cgctgatgct cgacgaggcg ggcggtatac ggagcgatat
      601 aaccgtcgcg cggctcggcg aggagacctt ccagctcggc ctgaacgggc cgcgggacat
      661 cctctggatg gaggaccatt tgccggagga cggctcggtg catgtccggg acgttaccgg
      721 tagttcctgc tgcatcgggg tgtgggggcc gctggcgagg gatctggtgc agccgctcac
      781 ggacgacgac ctctcgaacg aggctttcgg gtacttcaag gcgcgacgga tctatataag
      841 agaagtgcct gtaaccgcgc tgcgcgtctc ctacgtcggg gagctcgggt gggagctcta
      901 cgcgccgacg gagatgggtt tgcggttgtg ggacctactg tgggatgccg gacagccgct
      961 cggggtgatc gcgggcggcc ggagcgcctt cggcagcctg cgcctggaga agggctaccg
     1021 gctctggggc accgacatgt ccaccgagca cgacccctac gaggcgggcc tcggcttcgc
     1081 cgtgaagccg gacaagggag acttcgtcgg ccgggaagcg ctcattcgtc gcagagaaga
     1141 tggactgcgc cggaagctga gttgcctggt gctcgaagac ccgaacgtag tggtcgttgg
     1201 caacgagccg gtctactcgg ggggtgagcc ggtcggctat gtcacgagcg cggcttacgg
     1261 gtacagcatc ggcaagagca tcgcgtatgg gtggctgccg ccggagcttt cgcgggtggg
     1321 gacccgggtg gaggtcgagt acttcgggga gcggcacggc gctaccgtgt cagccgaacc
     1381 tctcttcgac ccggagatgc ggcacctggg tgtcgtacgg aagagcccgg agcccgccgc
     1441 gcgggctgca tcctacgcga gagtcccggc ggacggcgga gtttgatggc gggtgttgat
     1501 cacaccggtc ctatgtatat gtctggactg tccgctctgc gtatgccgga aggcgtagtc
     1561 gcttatggag agagccagat aaaggaagga gagcggactg cgcccggtag atgcggttaa
     1621 caaaagcggt tccgtaaggt cggtaaacag ggctgtcgcc ataatgaggt ttctctccag
     1681 tcatgggcct gcaaccctga cggaggtctc ggacggcgtg gagatctaca agtccaccgc
     1741 tcatcgtctg ctcgcgactc tgcgggacga ggggctggtc gagcaggacg ccgcgaccac
     1801 caggtacagt ctgggttacg ggctggcgct tctggcaagc tctgtaacat ccggcccgga
     1861 catcctgcgc tgcgcccggc cggtctgcga gcgtctcagc gaggagacgg gggagacggt
     1921 tacggtcgcg gtcctggaga acaacgaggc cgtcgtgatc caccaggcgc tatctaactc
     1981 ttccgcgctg agcgttgact ggacggggag gcacacgccc atacacgcta cgggcgcggg
     2041 aaaggtgttc ctcgcgcaca tgccggagag ccagctagat agcatcatgg tagaccctct
     2101 ggagcgcttc acggagcata ccgtggtaga gcctgcgcgg ctgcgggagg atctggagga
     2161 catccgggcc agaggctacg ctatctgttt cgaggaactg gagcccggcc tgaacgtgat
     2221 cgcggctccg gtgcggttct cgcagggacc cgtgaccgct gccatatcgg tctccggtcc
     2281 ggccttccgg ttcccgccgg acggcgccga ggcagtcggc gagttgacct gcggggccgc
     2341 cgacgagata tccaggtgtc tggggttctg ggaatgatgg gctcgccggg gcgccggatg
     2401 cctgtgagtg tcaaagaggc gcgtgaattg acagttggcc acacattcgt cagaattcgc
     2461 tgtgcgctgg ggatggtatc ggcgggatga gggggcaggc aataaggcgc gcgggcctcg
     2521 tcttgttcgc tacgcttcgc gaccatgaag agacgatgta cagcaccgag cgctagaggg
     2581 gattgcattt atgcgccgca ctgagcatca ggtaacggca tgaacagtct gagctttccg
     2641 gactacctgg cgtcgcgctg ggatgacctc ctggagcttt cgatagagca cgttcaggtg
     2701 gtgttgatct cgttgttgat cgcgacggtc ataggggtct cgcttggaat ccttacctac
     2761 cggcgtccg
//
LOCUS       DASSMB010000180        18472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_551586, whole genome shotgun sequence.
ACCESSION   DASSMB010000180 DASSMB010000000
VERSION     DASSMB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 18472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18472
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551586"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFJ72_10665"
     CDS             <1..657
                     /locus_tag="VFJ72_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7479962.1"
                     /translation="ARLVVTDVDEAGLQGLKEKLEGSGAEVEAVVGDVSVVEDARRMI
                     SAATERFGRLDVLVANAGVIPLNNVVDATPEDWDHVMAVDGRGMFLTCKFAVEAMLEN
                     GGGSIVCLSSISGLAGQKEQSTYGPAKFVASGITKHLAVEWADRGIRVNAVAPGTIQT
                     EAVAELPEEYKAPMKEAHPIGRLGEPYEVANAILFLASDEASFVTGAILPVDGGYLAQ
                     "
     gene            667..1863
                     /locus_tag="VFJ72_10670"
     CDS             667..1863
                     /locus_tag="VFJ72_10670"
                     /inference="COORDINATES: protein motif:HMM:NF018682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HET7479963.1"
                     /translation="MEIAVIADDLTGAADTGVQLTRAGYRTAVAFREAEMPPAGALDA
                     VALDTDSRTMPLRTARERVSEAGGAVRDARIVYKKIDSTLRGHVAAEISAALEATGRE
                     RAVVAPAFPASGRTTVGGVQLVHGVPVHETEMRNDPRTPVREGHIPTLLAESFPSVET
                     LSVEDLADPASARRTIEDAGCVVADAVRDGDLEALVRVVPDPSDVLWTGSAGLAGALG
                     AVYPGSHVGATPASTPARAVVVVIGSLSGVARDQLRGLVEEYGEVAVPVEARRDGVVE
                     KTAEAARRVLSEKACVILHSPEKRDPSGGAGPVVTTLAKVAARLSKRGLFDALVLTGG
                     STAVGVARRLGASGIRLEGEVEAGVPVGTLIGNSPYRVVTKAGGFGRSDTLVRAVEML
                     LDNGKD"
     gene            1871..2881
                     /gene="pdxA"
                     /locus_tag="VFJ72_10675"
     CDS             1871..2881
                     /gene="pdxA"
                     /locus_tag="VFJ72_10675"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET7479964.1"
                     /translation="MTQPLVALTMGDPAGIGPEIIARMLADPDFHTENRAVVVGDAKI
                     LERAARLLNLPLRINAIVEPEEALFEPGAADLIQVGDVPEDLPFGELDARAGDAAFRY
                     LERAIHLASAGRVAAVATAPLNKEAMHLAGHKYPGHTEILAELTGTKDYAMMLVTDEL
                     KVIHVSTHVALREAIERATTERELAVIRLAHESLKKLGVENPKVAVAGLNPHAGENGL
                     FGTEDAERIAPAVAAAVEEGIDASGPHPPDTVMMRARTGVFDIVVVQYHDQGHIPIKL
                     MGFDTGVNVTVGLPFFRTSVDHGTAFDIAGTGKADPTSLRAALDLARKLAGAEERAEE
                     RS"
     gene            2884..4209
                     /locus_tag="VFJ72_10680"
     CDS             2884..4209
                     /locus_tag="VFJ72_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET7479965.1"
                     /translation="MEFPKVVKVRRKFPRPRVEDVEETVREQLAREEIAGTIRPGMSV
                     ALTAGSRGIAEIDGILRSLVEGLKELGAEPFIVPAMGSHGGATAEGQVEILESLGVTE
                     ESCGAPIRSSMEVVELGETDRGVPVYMDRIASEADGVVVVNRIKAHTDFRSDIESGLL
                     KMASIGLGKHHQALALHGYGVEGIRDFMVEVGEKVLESWHVLFGLATVENAYDEPAII
                     EAILPADIPQRERELLAEYMEMMPDLPVADIDVLHVDALGKNYSGTGMDTNVIGRFRI
                     LGVEEPERPNVKYLIVGDISEESHGNALGIGLADLTTTRLAERIDQKAMNENVLTSTF
                     IERAKIPMVMANDRKALEAAIRCNWGVPPEETRFVRIPNTLHLEYMYVSENLLEEALG
                     HDDLEVVGEAADLAFDDNDNLTPFDAVEGEGHAVAASSAAEDGYYPGDL"
     gene            4606..5409
                     /locus_tag="VFJ72_10685"
     CDS             4606..5409
                     /locus_tag="VFJ72_10685"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7479966.1"
                     /translation="MDLEQLGGMRVVDLSLTLSERLPGTWPGHMIFAHKNWNWFDEVD
                     GPVEKTISNAPYQTNFVVIDEHCGTHFDAPTHFIPPPDSGLPYASDLGNQTGDKVPLE
                     DLMGPAAVVDVSYLSGEGEPGVSPFIQPEHVQAFEQEHGELRRGEIVLFKTNWDRYYV
                     EGEEGEKYMHGSLVTGEFPGWPAPSPETAIYLHEKGVKTIGIDAPSIGSAHDGVPVHQ
                     EGLSREMRYIEILTNLDQLPVRGAYFVFLPIKIGRSSGGPGRAMALLPG"
     gene            5494..6726
                     /gene="glp"
                     /locus_tag="VFJ72_10690"
     CDS             5494..6726
                     /gene="glp"
                     /locus_tag="VFJ72_10690"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET7479967.1"
                     /translation="MQLFEKLIEYPEAERLVLENTPLLPAEETPLGDALGLVLAEDLP
                     ATVDSPPFDNSAVDGYAVRSADAAEGRVFRVVDEAPAGRPAERSVGEGEAIKIFTGGV
                     IPEGADAVVMVENTSGWGEEFELKKVPDPGNNIRAAGEDARKGDIILRAGIGIGAPEI
                     AFAATQGYGALPVYRRPKVVVLSTGTELVEPGTRELSPGEIYDSNSFAIVAQAGEAGA
                     EARRVHAASDDADVLRAALEEALESADVVVTSGGVSVGEKDLVKSTLLELGVEQVFWG
                     VKFKPGKPLFYGARGETRFFGLPGNPVSAMVCFDLFVRPALMRMMNREDGERPRIRAY
                     FEEDVKNTFGRMHAMRVSLERTEKGWRAESVGAQGSGLVSSLTKADALALIGPESEGV
                     RAGEPVEAIVLREWVVRD"
     gene            6853..7890
                     /locus_tag="VFJ72_10695"
     CDS             6853..7890
                     /locus_tag="VFJ72_10695"
                     /inference="COORDINATES: protein motif:HMM:NF025873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7479968.1"
                     /translation="MFESDHEDNVFRRRLMKEVAPGLPVHFSFYSPSVKVQVMYPAEH
                     MPGANGRYVGNGVTIEFVPEDTAAVERVREYVWHWEEYNAFTAIRKINHLRRSVCPGS
                     VEGALERVRGVDRRAAAKAAEALDYEELRDMIDTEDVKALARFPGLGEKRAKAVVEFY
                     RNERSGRRFLYAANRNDRFRGQPVVTELDLSDDIEEQVWEHAGHARRLGDPDPLSGNE
                     PPGHTTVRGANLLHPLPMTPSLMGRGVYQPEQVELFADALRRVLVEGEKLAGVDDLRI
                     QRGKIFHTEKMPGIGMKTRARVLASGLLDDEYSFESLVSIPGVTEAQARTIARTAKVV
                     ASHTRAEVRAS"
     gene            7880..9712
                     /locus_tag="VFJ72_10700"
     CDS             7880..9712
                     /locus_tag="VFJ72_10700"
                     /inference="COORDINATES: protein motif:HMM:NF005906.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HET7479969.1"
                     /translation="MPPSLYGFVRENGPVSPELVASELLALDLNGDARPYIEKIVGDD
                     PRFLWEGENLLAVDPASLALEEALYVVFDVETTGSSAKEGGITEIGAVKVAGGVILDE
                     FSTLMNPERPIEPFVMRLTGITNRMVADAPLASEVMPRFEEFAEGAVLVGHNVHFDCA
                     FVEAARAANGLTPLTNPILDTLKLARALVPGLRRYRLSALVTHFGVRQLPNHRALADA
                     SATAEVLLKLLKLLKSAGVRSIGEAATIRGAGRGRIKPQKQHLAENIPHTPGVYYFVD
                     KHGTVLYVGKAKDLKARVRTYFNGGDGRRKVGRLVEEVAEVRFTETGSELRALILESR
                     EIRRLLPRYNSAGRGEQASWFIKLDLNEPYPVPVRVSTNDPVDGVTYLGPYRSAGVLD
                     TCIEALGRVFPLRRCENGAADEVCFYGQMGRCAPCLGMSAEEYRSEVLNEMVALMRGE
                     GGEAHLAALIEERARLAKELEFEAAARLRDLIAGIERIRLTRAVVSAEGVQVVVAPST
                     EPGVVEVFALAGGRLVAHGGFEAADLAGISRFATEALASGETNTGRSSARPEGSDEAR
                     VVAAYLRRRQTPIEAVRLRCVEDLLKAVKRVAEAEAESNVASLA"
     gene            9797..10495
                     /locus_tag="VFJ72_10705"
     CDS             9797..10495
                     /locus_tag="VFJ72_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479970.1"
                     /translation="MVQNLQGMTRRILLVDDEPSIQKMLVHALEREGFQVHPVGDGEE
                     ALEAFGSYEPHLIILDIMLPKIDGTEVCRRIRSQSDVPIIMLTAKDDEIDRVVGLELG
                     ADDYVTKPFAVRELVARVRAIMRRATVPAGQRQDELSYDGLRINIPSRRVAVEGKEVD
                     LTYTEFELLVTLASNPGRVFSRSTLLTRVWGDEFRDERTVDVHIRHLREKIERDARNP
                     EFIHTARGVGYVFR"
     gene            10590..12296
                     /locus_tag="VFJ72_10710"
     CDS             10590..12296
                     /locus_tag="VFJ72_10710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7479971.1"
                     /translation="MRIWLTALFMLVTGFAAVTAYEIVRPIMEDSLTRASEAAFAQVG
                     QQFESEIARDPDFSTPRIRAFATSRGLQWGIVQADGGKKLKGDPNLEWIPGVVKKAVE
                     ERRGVSNIVPVKSGDRSGQIQATYAAPITVDNEKGTAVVFNAFYTESDVRNAAKALDK
                     IESIAFLAGVLALIIAGVAGYVAATLISRRVSRLGLAAERLAAGNFDERIKTRIEDEV
                     GTLGTAFNSMAVSLKSAFGQVEQEKKRGGAILDGMTDGVIGVNKDLDAIFLNPRAREI
                     LESSDLAFHTRLQEILAKSRYSGPVTEPEADAGDRIIEIRAAPLEDGALAILRDVTEE
                     RRIQRAKAAFIANASHELKTPLFALSGYLEMIEDEEDEKVRADFLEGMRTATERLQNL
                     AKTLLDLSRLDANAVTFRIEELYLEDLLHELRRDFEYTGRPLRLHVESDVPAVETDPN
                     QLHRTLTILLDNALKYSEDGTPVDLSLHRENGNAVISVTDRGCGIPEPEQPHIFERFY
                     RAEGSSRADGTGLGLALAQEIMHHLGGEIRVQSKPNAGSTFSVVLPLSEKAPLSRTLT
                     HS"
     gene            complement(12424..13071)
                     /locus_tag="VFJ72_10715"
     CDS             complement(12424..13071)
                     /locus_tag="VFJ72_10715"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7479972.1"
                     /translation="MFLTLFEQNPAAAVAVALGFVVGIVLHEAAHAYSAYKLGDDTAY
                     RAGRVTLNPASHLDVLGTLMLLMAGFGWGKPTPVVPSKLRGGVFGPVAVAFAGPLSNL
                     IIVAVCAALFLLPPFQSGYLAIIIAAVAFVNALLFVFNLIPIPPLDGSKVIFPFLPRA
                     LDGFVDFMNQYGPMILFGLILLSFVIPGFSPFSFILTLILPILTLFGLPPGIPAA"
     gene            complement(13124..13906)
                     /locus_tag="VFJ72_10720"
     CDS             complement(13124..13906)
                     /locus_tag="VFJ72_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479973.1"
                     /translation="MAEPARSGEGRRPGNASDRIAERREARKVAQESVYRRRRVLAVC
                     LLVLGVLALVVAVFAGTSGATERAVPIDPNNAGPDTVLAEAAGIDISSPIRPANITGI
                     GYHPEGESLIEMAPRGRNLSSNALFNLFSGGSTPENIQYHMMDPAGRPGFRTGAMDVG
                     AEAGSTVYAPITGTVTAIRPDPMMQGANIVEIKSSEDSDVRVSVSLVRDISEGIGPDK
                     PVKAGMTELGAVADSSKVLTPQLSQITNDAGNHVTVSATRVG"
     gene            14087..15304
                     /locus_tag="VFJ72_10725"
     CDS             14087..15304
                     /locus_tag="VFJ72_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7479974.1"
                     /translation="MPARKLLVSTYLQYILLALGVVLLFSFVRQIGGVLVTFLMAGIL
                     AYVLNPLVRRLERRIPRVVAVVGIFLILAVAVVTALLVLIIPAVGQVQRLVQNPAAAV
                     DAVNALVERAQDLPYVGERIASLDQKAVTQFLQKNAPSAGQTLNVALGFIGGVFGVFG
                     TILNLILMLIVSIYLLLDRERITRAVIGAIPDTIRDQAVELFHAVEDALIKYIRAQLT
                     LCAIMGVIGWAIGQFAIGKYALLIGLWVGLTEIIPVLGAFLGAAVAILIALIVPDGGI
                     TTALIVAGLFLLAQQLEGNILVPRVMGGSVGVHPLWVLFATLAATALYGVVGAIFAVP
                     IVAIIAATFRYLRGTLVFEEWGNAPIRPLAEGGDEKLPVGAPESESPSAAAGLGRSRR
                     PDGSSIARGREKD"
     gene            15308..15928
                     /locus_tag="VFJ72_10730"
     CDS             15308..15928
                     /locus_tag="VFJ72_10730"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7479975.1"
                     /translation="MSVPHREPRRPFSYKESLRQVLNPLVRLLSRMRVRPDTLTIFGW
                     ALSLVSAVLFGLGYIQIAGAVMLFGGLFDALDGAVARASDRMSDFGAFLDSTLDRISE
                     AVIFVGIIFYYAASGRPYETMLAGAAMSFSLLTSYTRARAEGLGVRCEVGLLERAGRV
                     LLLSVLSIFGLLTVGVALVAAGALITTAQRILHVRRETRSYPDTRS"
     gene            15925..16443
                     /locus_tag="VFJ72_10735"
     CDS             15925..16443
                     /locus_tag="VFJ72_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479976.1"
                     /translation="MSARVSARAPRDAASDYRAKLSHEAARLARKMREAGHQPPLGDP
                     GCGLMVVIEQPVGPRVLDALSRSLSAIGLSDAYVTAAGTGRLYEEALSTEPSALVAVG
                     PGAAQGIDALDHPLALRSYSDATQGQWFDWTKGTAGLLLPPLAPALDDEEAKRRFWTS
                     FLALRQLAPRAL"
     gene            16446..17198
                     /locus_tag="VFJ72_10740"
     CDS             16446..17198
                     /locus_tag="VFJ72_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479977.1"
                     /translation="MPRLPAARLFCILGLAALAALTVSLPFADLQPSFTAHVVSYALA
                     LVPVAALIYGFPDLVRDLATPRLLTLGAVSATLGFLAALACRPDAFDAGPRLLPLALL
                     YLANVLRLCAAASLGLALARYVISPGVALLIAVLAAVSDLFSVLAGPTKVLLSKGSPA
                     LDFLILVFPSFGAPLGFGFGLSDFVFLALFAYISLLLGLRYQTTLLFCSAAAMLAAVT
                     ALSLEQPLPALPFICLGFVLANIWQILARMRK"
     gene            17472..>18472
                     /locus_tag="VFJ72_10745"
     CDS             17472..>18472
                     /locus_tag="VFJ72_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479978.1"
                     /translation="MFLGFVTVIVFATGISAGAAPQGDTQSKQAEVSSAQDRLTSIRQ
                     EASSSYEEYNNALFQLNELDKELDATNQDLEKAQSRLTEAQSNLEERASQVYRSGNVG
                     FVDVLVGVDNFSDFATRLDLWMKLLGQERAEFETVLEAKNDIAAKQASLEDQRSQRAS
                     ALKDAAERKKQAENAQTEAQAYLDSLNADLQAAIEADAQKQAEAARAAAEKAAKSESA
                     PKAAKSESAPKAQPELVQIADVQPAAQQAAPQASRADLKSQQQAAAQRAAERAAARAA
                     AEKAAAEAERQAKLAQQEATQKKADQRAAAEQRAEERQAAAQQARDAAEQAAIEEAAA
                     "
BASE COUNT         3325 a         5735 c         6274 g         3138 t
ORIGIN      
        1 gcgcggctcg tcgtgacgga cgttgacgag gccgggctcc aggggcttaa agagaagttg
       61 gagggttcgg gggctgaggt ggaggcggtc gtcggggacg tctcggtggt cgaggacgcg
      121 aggaggatga tctctgcggc cacggagcgg ttcgggcggc tggacgtact ggtggcgaat
      181 gccggagtca tcccgctcaa caacgtcgtg gacgcgacgc cggaggactg ggatcacgtc
      241 atggcggtgg acgggcgggg gatgttcctg acctgcaagt tcgccgtcga ggcgatgctg
      301 gagaacgggg gaggctcgat agtctgcctc tcgtccatct ccgggctggc gggccagaaa
      361 gagcagtcca catatggtcc ggccaagttc gtggcgtccg ggatcaccaa gcacctcgcg
      421 gtcgagtggg cggaccgggg catccgggtc aacgccgtgg cgcccggcac catccagacc
      481 gaggccgtcg cggagctgcc ggaggagtac aaggccccga tgaaggaggc gcaccccatc
      541 ggccggctcg gcgagccgta cgaggttgcc aacgcaatcc tgttccttgc ctccgacgag
      601 gcgtcgttcg tgaccggcgc gatcctgccg gttgacggag gatatctggc gcaataaggg
      661 acggtagtgg agatagccgt catcgccgac gacctgaccg gagccgccga caccggagtg
      721 caactcaccc gcgccggata ccggacggcg gtcgccttcc gggaagcgga gatgccgcct
      781 gccggcgccc tcgacgccgt ggcgctcgac accgactccc gcacgatgcc gctgcgcacg
      841 gccagggagc gcgtctcaga ggcggggggc gcggtgcggg acgcccgcat cgtctacaag
      901 aagatcgact ctacactacg cggtcacgtc gcggcggaga tctccgccgc gcttgaggcg
      961 accggccggg agcgcgcggt cgtcgcgccg gccttcccgg cctccgggcg aaccacggtc
     1021 ggcggcgtgc aactcgtaca cggcgtgccc gtccacgaga ccgagatgag gaacgacccg
     1081 cgcacccccg tgcgcgaggg acacatccca accctgctcg cagagtcgtt cccttcggtc
     1141 gagaccttga gtgtcgaaga ccttgcagat cccgcgagcg cgcgccggac catcgaggat
     1201 gccggatgcg tagtggccga cgcggtgcgg gacggggacc tggaggctct ggtccgggtc
     1261 gtgccggacc cttccgatgt gctctggacg ggctccgcag ggcttgcagg cgccctcggc
     1321 gcggtgtatc cgggatctca tgtaggcgcg actccggcat caactccggc ccgcgctgta
     1381 gtggtcgtga tcggcagcct gagcggggta gccagggatc agctccgtgg gctcgtggag
     1441 gagtacggag aggtagcggt gcccgtcgag gcccgcaggg acggggtggt ggagaagacc
     1501 gcagaggctg cgcggagggt cctgtcggag aaggcttgcg tcatcctgca ctcgcccgag
     1561 aagagagacc cgtcgggggg cgcggggccg gtggtgacga cgctcgcgaa ggtggcggcg
     1621 cgcctctcga agcgggggct gttcgacgcg ctcgtgctaa cgggagggtc gacggccgtc
     1681 ggggtggcgc ggcggttggg agcgtccggg atacggctcg aaggcgaagt ggaggcgggc
     1741 gttcctgttg ggactttgat cgggaacagt ccgtaccggg tggtgacgaa ggcgggcggc
     1801 ttcggaagat cggatacact tgtaagggcc gtcgagatgc tcctggataa cgggaaggat
     1861 tagagagcaa atgacccaac ccctcgtggc gctgacgatg ggagaccccg ccggtatcgg
     1921 gccggagatc atcgccagga tgctcgccga cccggacttc catacggaga atcgggccgt
     1981 cgtagtgggc gacgcgaaga tcctggagcg cgcggcgcgg ctgctgaatc taccgctccg
     2041 gatcaacgct atcgtggagc cggaggaggc cctgttcgag ccgggcgcgg ctgacctgat
     2101 ccaggtcgga gatgtcccgg aggacctgcc tttcggagag ctggacgctc gggccgggga
     2161 cgccgccttc cgctacctgg agcgggcgat acatctcgcg agcgcgggcc gcgtggcggc
     2221 ggtggcgacg gctcccttga acaaggaggc catgcacctg gccggtcaca aatatccggg
     2281 gcacaccgag atcctggcgg agctgaccgg cactaaagac tacgcgatga tgctcgtcac
     2341 cgacgaattg aaggtcattc acgtctctac ccacgtcgcg ctccgggagg caatcgagcg
     2401 ggcgaccacg gagcgggagc tggccgtgat ccgtctcgcc cacgagtcgc tgaagaaact
     2461 cggcgtcgag aacccgaaag tggccgtcgc cggcctcaac cctcacgcgg gagagaacgg
     2521 tctcttcggg accgaggacg cggagcggat agcccccgcc gtggccgctg ccgtcgagga
     2581 ggggatagac gccagcggcc cgcatccgcc ggacaccgtg atgatgcggg cgcggacggg
     2641 cgtgttcgac atcgtggtgg tgcagtacca cgaccagggg cacatcccca tcaagctgat
     2701 gggtttcgac accggagtca atgttacggt cggcctgccg ttcttccgca ccagcgtgga
     2761 ccacggcacg gccttcgaca tcgccggtac gggaaaggcg gacccaacaa gtctccgcgc
     2821 cgctctggat ctcgcccgca aactggcggg cgccgaggag cgcgcggaag agaggagcta
     2881 gaggtggagt tcccgaaggt agtgaaggtg cggcggaagt tcccccgtcc gcgcgtcgag
     2941 gacgtcgagg agacggtcag ggagcaactt gcgcgcgagg agatagcggg caccatccgg
     3001 cccggcatga gcgtagcact cacggctgga agccgcggca tcgcggagat agacgggatc
     3061 ctgcgctcgc tcgtcgaggg tctgaaggag ttgggggcgg agccgttcat cgtcccggcg
     3121 atggggagcc acggcggggc gacggccgag gggcaggtcg agattctgga gagcctcggg
     3181 gtgacggagg agtcctgcgg cgcgcccata cggtcctcca tggaggtcgt ggagcttggg
     3241 gagacggatc gcggggttcc ggtgtacatg gaccgcatcg cctcggaggc ggacggcgtg
     3301 gtcgtggtca acaggatcaa ggcgcacacc gacttccgct cggacatcga gagcgggttg
     3361 ttgaagatgg cctccatcgg cctcggcaag caccatcagg cgctcgcgct gcacggctac
     3421 ggcgtggagg gcatccggga cttcatggtc gaggtcgggg agaaggtgct ggagagctgg
     3481 cacgtcctct tcgggctcgc aaccgtcgag aacgcctacg acgagccggc gatcatcgag
     3541 gcgatcctgc ccgcggacat cccccagcgc gagagggagc tgctcgccga gtacatggag
     3601 atgatgccgg acctcccggt agcggacata gacgtgctgc acgtggacgc gctggggaag
     3661 aactactccg gcacgggaat ggacacgaac gtcatcggcc ggttccgcat cctgggcgtc
     3721 gaggagcccg agcggccgaa cgtgaagtac ctgatcgtgg gcgacatcag cgaggagtcg
     3781 cacggcaacg ccctcggcat cgggctggcg gacctgacga cgacgcgcct ggcggagcgg
     3841 attgaccaga aggcgatgaa cgagaacgtc ctgacgagca ccttcataga gcgggcgaag
     3901 ataccgatgg tgatggcgaa cgaccggaag gcgctcgaag ccgccatccg ctgcaactgg
     3961 ggcgtcccgc cggaagagac gcgcttcgtc cgcatcccga acacgctgca cctggagtac
     4021 atgtacgtct ccgagaacct gctcgaagag gcgctcggcc acgacgatct tgaggtggtt
     4081 ggcgaggcgg cggatctcgc gttcgacgat aatgacaacc tgacgccctt cgacgcggtg
     4141 gagggcgagg ggcacgccgt cgcggcgtca tccgccgcgg aggacggtta ctacccgggg
     4201 gatctgtagt ggcgtggagg agcgctgcac agacgatgcc ttcaagcgtg caattttcat
     4261 gaacctacgt taaggggcgg catggctaga ccaccgagaa gagagataag gccagcgagc
     4321 ctaaagtgta ataactgtgg acacgagttc gtggaccggg cgtggtttgt gtcaattgaa
     4381 tctgagcgcg ggacaatagg atggaaccaa gatacggacg ggactagagg cactgagtgt
     4441 ccctcgtgcg gctccaattt gatcggattt cgaagagact gaccactcac atgctctgac
     4501 caagatgccc gaatatttag gcaaagcgta gtttacctgt gcagaaggat tttatgtgca
     4561 agattgcgcg gagggatacc cttcgcccgg acaggaggag ataaggtgga tcttgaacag
     4621 ctaggcggca tgagggtggt ggacctctcc ctgaccctct cggagcggct tccgggaacg
     4681 tggccggggc acatgatctt cgcccacaag aactggaact ggttcgacga ggtggacggc
     4741 ccggtcgaga agaccatcag caacgcgccg taccagacga acttcgtcgt catagacgag
     4801 cactgcggca cccacttcga cgccccgacc cacttcatcc cgcccccgga ctccgggttg
     4861 ccctacgcga gcgacctcgg caaccagacc ggcgataagg tgccgctcga agatttgatg
     4921 ggccccgcgg cggtcgtgga cgtgagctac ctctccggcg agggagagcc gggcgtgagc
     4981 ccgttcattc agccggagca cgtccaggcg ttcgagcagg agcacggaga actccggcgg
     5041 ggcgagatcg tgctgttcaa gaccaactgg gaccgctact acgtcgaggg cgaggagggg
     5101 gagaagtaca tgcacggctc gctcgtcacc ggcgagttcc ccggctggcc cgcgccttca
     5161 cctgagaccg cgatctacct gcacgaaaag ggcgtcaaga ccatcggcat agacgccccc
     5221 agcatcggca gcgcccacga cggcgtaccc gtccaccagg aagggctcag ccgggagatg
     5281 cgctacatag agatcctcac caacctcgac caactccccg tgcgcggagc ctacttcgtc
     5341 ttcctgccca tcaagatcgg tcgctccagc ggcggtcccg gacgggcgat ggccctcctg
     5401 cccggctaaa acgcaaagaa cctatcccgt ctccccgacg cgcggcgtat ggtagcgtac
     5461 cggggagacg tgttttggtt aggggagcga aggatgcaac ttttcgagaa gctcatagag
     5521 tacccggagg ccgagcggct ggtcctggag aacacgccgc tcttgccggc cgaggagacg
     5581 ccgctcggcg acgcgctggg actggtgctg gcggaggacc taccggcgac ggtcgactcg
     5641 ccgccgttcg acaactcggc ggtggacggg tacgctgtcc gcagcgccga cgcagcagag
     5701 ggtcgcgtgt tccgggtcgt ggacgaagcc ccggccggcc gtccggcgga gagaagcgtc
     5761 ggcgagggcg aggccatcaa gatcttcacc ggcggcgtca tccccgaggg cgccgacgcc
     5821 gtggtgatgg tcgagaacac ctccggctgg ggcgaggagt tcgagctgaa gaaagtcccc
     5881 gatccgggca acaacatccg cgccgccggc gaagacgccc ggaagggcga catcatcctg
     5941 cgggcgggga ttgggatcgg ggctcccgag atagccttcg ccgccaccca gggctacggc
     6001 gcgctgccgg tctaccggcg tccgaaggtc gtcgtgctct ccaccggcac cgagctggtc
     6061 gagccgggga cgcgggagct gtcgcccggc gagatatacg actccaactc cttcgccatc
     6121 gtcgcccagg ccggcgaggc cggcgcggag gcgcgtcgcg tccacgccgc ctccgacgac
     6181 gccgatgtcc tgcgcgccgc gttagaggag gcgctggaga gcgctgacgt cgtggtgacg
     6241 agcggcggcg tctcggtggg ggagaaggac ctggtcaaaa gcacgctcct ggagctcggg
     6301 gtggagcagg tcttctgggg cgtgaagttc aagcccggca agcccctctt ctacggagca
     6361 cggggcgaga cgcgcttctt cggcctcccc ggaaaccccg tctccgcgat ggtctgcttc
     6421 gacctcttcg tgcggcccgc gcttatgagg atgatgaacc gcgaggacgg ggagcgtccg
     6481 cgcatccggg cctacttcga agaggatgtg aaaaacacct tcggccggat gcacgcgatg
     6541 cgggtgagcc tggaacggac agagaagggc tggcgggccg agtccgtcgg cgcacagggg
     6601 tccgggctcg tcagctcgct gacgaaggcc gacgcgctgg cgctcatcgg ccccgagtcc
     6661 gagggcgtcc gcgccggaga gccggtcgag gctatagtgc tgcgcgaatg ggtagtaagg
     6721 gactgatccg ggcctcgcga aaacctcttg ccggaaacaa agctgttggc taaacgtaat
     6781 cttaccggta aggttgtgca ggctgatgtc tttcacaggg gagaggtacg gtagtggtag
     6841 aatttgggtc cgatgttcga gtctgatcac gaggacaacg tcttccgacg gaggttgatg
     6901 aaggaggtcg cgccgggact gccggttcat ttctccttct attcaccttc agttaaggtt
     6961 caggtcatgt atcccgccga gcacatgccc ggcgccaacg gccgctacgt cggcaacggg
     7021 gtgacgatag agttcgtccc ggaggatacg gcggcggtcg agcgggtgcg cgagtacgtg
     7081 tggcactggg aggaatacaa cgccttcacg gccatacgca agatcaacca cctccggcgc
     7141 agcgtgtgcc ccggcagcgt cgagggtgcc ctggagcgcg ttcgcggcgt ggaccgccgg
     7201 gccgccgcga aggccgccga agccctggac tacgaggaac tgcgcgacat gatcgacacg
     7261 gaggacgtga aggcgctcgc gaggttcccc ggtctcgggg agaagcgggc gaaggccgtg
     7321 gtcgagttct accgcaacga gcgctcgggt cggcgcttcc tgtacgccgc gaaccgcaac
     7381 gaccgcttcc ggggccaacc ggtggttacg gagctggacc tctcggacga catagaggag
     7441 caggtctggg agcacgccgg gcacgcgcgg cgtctcggag acccggaccc actgagcggg
     7501 aacgagccgc ccggacacac caccgtccgc ggcgcgaacc tcctgcaccc gctgccgatg
     7561 acgccctccc tcatgggacg cggcgtgtac cagcccgagc aggtcgagct gttcgccgac
     7621 gccctccggc gcgtgctggt ggagggggag aagctggccg gggtagatga cttgcggata
     7681 caacgcggca agatcttcca caccgagaag atgcccggta tcgggatgaa gacccgcgcc
     7741 cgggtgctcg cgagtggcct gctcgacgac gagtactcct tcgagagcct cgtctccata
     7801 cccggcgtga ccgaggcgca ggcgcgcacc atcgcccgga ctgccaaagt tgtagccagc
     7861 cacacgagag cggaggtccg tgcctcctag cctctacggc ttcgtccggg agaacgggcc
     7921 tgtaagcccg gagttagtcg catctgaact gctcgccctc gacctcaacg gcgacgcccg
     7981 gccgtacata gagaagatcg tcggcgatga cccacgcttt ctctgggaag gagagaacct
     8041 tctggcggtg gaccccgcct cgctcgcgct cgaagaagcg ctctatgtag tcttcgacgt
     8101 ggagacgacg ggctcgtcgg ccaaagaggg cggcatcacg gagatcgggg ccgtcaaggt
     8161 cgcgggcggt gtgatcctgg acgagttctc cacgctcatg aaccccgagc ggcccatcga
     8221 gccgttcgtc atgcggctca ccggcatcac caacaggatg gtcgcggacg cgccacttgc
     8281 ctccgaggtt atgccgcgct tcgaggagtt cgcggagggc gcggtgctcg tcgggcacaa
     8341 cgtccacttc gactgcgcct tcgtcgaggc ggcgcgcgcg gcgaacgggc ttactcccct
     8401 tacgaacccg atcctcgaca ccctgaagct cgcccgcgcc ctcgtgccgg gcctcaggcg
     8461 ttaccgtctc agcgcgctcg tcacccactt tggtgtgcgc cagctaccga accaccgcgc
     8521 cctggccgat gcttcggcga ccgcggaggt gctgctgaag ctcttgaagc ttctcaaatc
     8581 cgctggagtc cggagtattg gtgaggcggc gaccatacgg ggggcggggc gggggaggat
     8641 caagccgcag aaacagcacc ttgcggagaa catccctcat acacccggcg tctactactt
     8701 cgtggacaag cacggcacgg ttctctacgt cggaaaggcg aaggacctga aggcgcgagt
     8761 caggacgtac ttcaacggcg gggacggacg gcgcaaggtc ggccggctgg tcgaggaggt
     8821 cgccgaggtg aggttcaccg agacgggtag cgagttgcga gccctgatcc tggagtcccg
     8881 agagatccgt cgcctcctcc cccgctacaa ctccgccgga cgcggcgagc aggcgagctg
     8941 gttcataaag ctcgacctga acgagccgta cccggtcccc gtgcgcgtct ccacgaacga
     9001 ccctgtagac ggcgtcacct acctcggccc ctaccgcagc gccggggtcc tggatacgtg
     9061 tatcgaagcc ctgggccggg tcttcccgct gcgccgctgc gagaacggag ccgccgatga
     9121 ggtctgtttc tacggacaga tgggccgctg cgcgccgtgc ctcgggatga gcgccgagga
     9181 gtaccggagt gaggtgctga acgagatggt cgccctgatg cggggcgagg gtggcgaggc
     9241 gcacctggcg gcgcttatcg aggagcgggc gcggctggcg aaggagttgg agttcgaggc
     9301 tgcggcgcgg ctgcgggacc tgatcgccgg tatagagcgt atccggctga cgcgggcggt
     9361 cgtgagcgcc gagggcgttc aagtagtcgt ggccccttcg acagagcccg gcgtcgtcga
     9421 ggtcttcgcg ctggcgggtg ggaggctcgt cgcgcacgga gggttcgagg cggcggacct
     9481 ggccgggatc tcccgtttcg cgacagaggc gctcgcgagt ggagagacga acaccgggcg
     9541 ttccagcgcc cgtccggagg gctcggatga ggcacgggtc gtcgcggcgt atctgaggcg
     9601 gcgtcagacg cccatcgagg cggtacggct gcgatgcgtc gaggacttac tgaaagccgt
     9661 gaagcgagtt gcggaggccg aggcagagag taatgtagcc tcgctagcat gatagcttgc
     9721 atggtaaaat acggatcaag taagtcgatg ttatccggca ggttgccggg cgtaggcagg
     9781 aagagacggt agcgagatgg tgcagaacct gcagggcatg acgcgcagga tacttctggt
     9841 ggacgatgag ccgtccatcc agaagatgct ggtgcatgcc cttgagcggg aggggttcca
     9901 ggtgcatccc gtgggagatg gcgaggaggc gctggaggcg ttcgggtctt acgaaccgca
     9961 cctcattatc ctggacatca tgctgcccaa gatagacggc acggaggtgt gccggcgcat
    10021 ccggtcgcag agcgacgttc cgatcattat gctcacggcc aaagacgacg agatagaccg
    10081 ggtcgtgggg ctggagctcg gcgccgacga ttacgtgacc aagccgttcg ccgtgcgcga
    10141 gctggtggcg cgggtccgcg ctatcatgcg ccgggcgacc gtaccggcgg ggcagcggca
    10201 ggacgagctc tcttacgacg gcctgaggat caacatcccg agtcgccggg tcgccgtgga
    10261 gggcaaggaa gtggacctca cctatacgga gttcgagctt ctcgtcaccc tggcctcgaa
    10321 tcctggcagg gtattctcgc gcagcacgct gttgacccgg gtctggggcg acgagttccg
    10381 cgacgagcgc acagtagacg tccacatccg tcacctccgc gagaagatcg agcgcgacgc
    10441 ccgcaacccc gagttcatcc acaccgcgcg gggtgtcgga tatgttttcc gctaagaaga
    10501 actctttcgg cagatcccac gttaaaggct cctggcgcag gctaaggaag cgcgcctctg
    10561 cgagaagggg gttccgtccg cgcatcagca tgcggatctg gctgacggcc ctcttcatgc
    10621 tcgtcacggg gtttgccgcg gtgactgcct acgagatagt gcgccccatc atggaggact
    10681 cgctgacccg tgcgagcgag gccgcgttcg ctcaggtcgg gcagcagttc gagagcgaga
    10741 tcgcccgcga cccggacttc tcgacgccgc ggatcagggc gtttgctacg agccgagggt
    10801 tgcagtgggg catcgtgcag gccgatggag gcaagaagct caagggcgac ccgaacctgg
    10861 agtggatccc cggcgtagta aagaaagccg tggaagagag aagaggcgtg agcaatatcg
    10921 tgcccgtcaa gagcggggat cggtccgggc agatccaggc gacttacgcc gcaccgatta
    10981 ccgtagacaa cgagaagggc acggctgtcg tcttcaacgc gttctatacc gagagcgacg
    11041 taaggaatgc cgcaaaagca ctggataaga tcgagagcat agcattcctg gcgggtgttc
    11101 tggccctgat catcgcgggc gtggcgggat atgtggcggc cacgcttatc tcccggaggg
    11161 tgagccggct gggactggcc gccgagcgtc tcgccgccgg taacttcgac gagcggataa
    11221 agacccgtat agaggacgag gttgggactc tcgggacggc tttcaactcg atggccgtgt
    11281 cgctcaagag cgccttcgga caggtcgagc aggagaagaa gcggggcggc gcgatcctgg
    11341 acggcatgac cgacggcgtg atcggcgtga ataaggacct ggacgctatc ttcctgaacc
    11401 cgcgcgcgag agagatcctg gagtcctcgg acctcgcctt ccacacccgc ttgcaggaga
    11461 tcctcgccaa gtcccgctac tccggccccg tcaccgagcc cgaggccgac gcgggcgacc
    11521 gtatcatcga gatccgggcg gctcctctcg aagacggcgc tctcgccatc ctgcgggacg
    11581 tgaccgagga acgccgcatc cagcgcgcca aggccgcgtt tatcgccaat gcctcccacg
    11641 agttgaagac cccgctcttc gcgctctccg gctacctgga gatgatcgag gacgaggaag
    11701 acgaaaaggt ccgggcggac ttcctggagg gtatgcggac tgccacggaa cgtctccaga
    11761 acctcgctaa gactctcctg gacctctcgc gtctggacgc gaatgccgtg accttccgca
    11821 ttgaggaact gtacctggaa gacctgctgc atgagttgcg gcgggacttc gagtatacgg
    11881 gacgcccgtt acgactccat gtcgagagcg atgtgccggc ggtagagaca gacccgaatc
    11941 agctccatcg caccctcact atactcctgg ataatgccct caagtactcg gaggacggca
    12001 cgcccgtgga cctcagcctt caccgtgaga acggcaatgc cgtcatcagc gtcacggacc
    12061 gcggatgcgg cataccggag ccggagcagc cgcacatctt cgagaggttc taccgcgccg
    12121 aagggtcctc ccgcgcagat ggtaccggcc tcggcctcgc gttggcgcag gagatcatgc
    12181 accacctcgg aggcgagatc cgagtccaga gcaagccgaa cgccggaagc actttctctg
    12241 tcgtactgcc cctctccgaa aaagcgccgc tctcccgcac cctgacccat tcttagcttt
    12301 tatctgaagt tgaggttacg cttcaggcac tttgaggtag aatatggaag ggatagcttt
    12361 cggtccgcag attctccccc ctccctgcgg gccggatcta tccctttttc ttccccgcaa
    12421 gggttatgcg gcggggattc cggggggtag cccgaagagc gtcagtatcg gcaggatcag
    12481 ggtcagtatg aagctgaacg gcgagaagcc cggtatgacg aagctgagca gtatgagccc
    12541 gaacaggatc atgggtccgt actggttcat gaagtcaacg aagccgtcca gcgcgcgggg
    12601 caggaacggg aagatgacct tcgacccgtc gagcggaggg atggggatga ggttgaagac
    12661 gaagagcagc gcgttcacga acgccaccgc cgcgatgatt atcgccaggt atccgctctg
    12721 gaagggtggc agtaggaaca acgcggcgca gacggcgacg atgatcaggt tggacagagg
    12781 tcccgcgaac gcgaccgcca ccggcccgaa gaccccgccc ctcagcttcg agggtaccac
    12841 tggcgtgggc ttgccccacc cgaatcccgc catgagcagc atcagggtgc cgagaacgtc
    12901 gagatgcgag gccggattga gcgtgacccg tccggcccta taggccgtgt cgtcaccaag
    12961 cttgtaggcc gagtaagcgt gcgccgcctc gtgcagcacg atgcccacca cgaaccccag
    13021 cgcaacggct acagcggcgg ccgggttctg ttcgaagagc gtcaggaaca taatctcacg
    13081 taatcataca cgcccggacg ggcggataga ggcgggaaga agactagccg actctcgttg
    13141 cagaaacggt tacgtggtta ccggcgtcgt tcgtgatctg ggagagttgg ggcgtgagga
    13201 ccttcgagga gtcagcgacc gctccaagct cggtcattcc ggccttaacc ggcttgtccg
    13261 ggccaatccc ctcgctaata tcccgcacca gagagaccga gacgcgcacg tccgagtctt
    13321 ctgaagactt gatctctacg atgttcgctc cctgcatcat ggggtccggg cggatggctg
    13381 tcacggtccc tgtgatcgga gcgtagacgg tactgcccgc ctctgcgccc acgtccatcg
    13441 cgccggtgcg gaagccggga cgtcccgccg gatccatcat gtggtactgg atgttctcgg
    13501 gagtcgaacc gccggagaag aggttgaaca gcgcgttgga agagaggttc ctgccccgcg
    13561 gcgccatctc aatgaggctc tcgccttcgg gatggtagcc aatgccggtt atattggccg
    13621 gacggatggg gctggagata tcgatgccgg cggcctcggc gagcacggtg tccgggccgg
    13681 cgttgttcgg gtctatggga acggcgcgct ccgtggcgcc ggaagtcccg gcgaagaccg
    13741 cgaccaccag cgccagcaca cccagcacga gcagacatac ggccagcacg cgccgccgcc
    13801 gatagacgct ctcctgagcc actttgcggg cctctctccg ctcggcgatc ctatcgctgg
    13861 cgttgccggg acggcggccc tctccagagc gcgccggttc cgccactgca aaccgctgtt
    13921 gcccaacatg ttgcgttctc tgcgtgttgc cccctctcat actgggggcc gattatacag
    13981 acgagggtgg ggactgtaaa cttccgcgag gtctttttga cgattgatca gcgagccgcc
    14041 caagactggt atatttgtag cgtagactca cccgaacgga gagccggtgc ctgcaagaaa
    14101 actgcttgtc tcgacctatc tgcaatacat actgctcgct ctgggcgtgg tgcttctgtt
    14161 cagcttcgtg cgccagatcg ggggcgtgct cgtcaccttc ttgatggcgg gtatcctggc
    14221 ctacgtgctg aaccccctgg tgcgtcgtct ggaacggcga atccctcggg tggtggcggt
    14281 agtggggatc ttcctgatcc tggccgtggc cgtggtgacg gccctgctcg tactaattat
    14341 cccagccgtt gggcaggtgc agaggctcgt ccagaatccg gccgccgcag tggatgcggt
    14401 gaacgctctc gtggagcgcg cgcaggattt gccctacgtg ggtgagcgca tcgcctcgct
    14461 cgatcagaag gcggttacgc agttcctcca gaagaacgcg ccctcggccg gacagacgct
    14521 caacgtcgcg cttggtttta tcggcggagt cttcggtgtc ttcggcacga tcttgaacct
    14581 gatcttgatg cttatagtct ccatctactt gcttctggac cgggagagga tcacgcgcgc
    14641 cgtgatcggc gctattccag ataccatccg cgatcaggcc gtggagcttt tccacgccgt
    14701 cgaggacgcc ctgatcaagt acataagagc gcagcttacg ttgtgcgcga tcatgggcgt
    14761 catcggctgg gctataggcc agttcgccat cggcaaatac gcgcttctca tcggtctgtg
    14821 ggtcgggctc acggagataa tcccggtcct cggcgccttc ctcggagccg ccgtggctat
    14881 actgatagcg cttatcgtgc cagacggagg tatcacgaca gcgctgatcg tcgccggcct
    14941 gtttctgctg gcccagcaat tggagggcaa tatcctggtg cctcgggtga tgggggggtc
    15001 ggtgggggtg catcctctct gggtcctctt cgccaccctg gccgctaccg ccctgtacgg
    15061 ggtggtcggg gcgatctttg ccgtacccat cgtcgccata atcgcggcca ccttccgtta
    15121 tctgcgcggc acgctggtct ttgaggagtg gggcaatgcg ccgatccgcc cgttggcgga
    15181 aggcggggat gagaagctcc cggtcggtgc tcccgaatcg gagtctccgt cagccgccgc
    15241 gggtctcgga cgctcccgca ggccagacgg ctcctcgatc gcgagaggga gagagaagga
    15301 ctaaagcgtg agcgttccgc accgcgagcc gcgccgccct ttctcataca aggagagcct
    15361 gcggcaggtc ctgaacccgt tggtgcggct cctctccagg atgcgggtgc gtccggatac
    15421 cctcactatc ttcggatggg ctctctccct ggtctcggcc gtcctcttcg ggctgggcta
    15481 catccagata gcgggtgccg tgatgctctt cggggggctc ttcgatgccc tcgacggggc
    15541 cgtcgcgcgc gcctcggacc ggatgagcga cttcggggcc ttcctcgact ccaccctgga
    15601 ccgcatctcc gaggctgtga tcttcgtcgg gatcatcttc tactacgcgg cctcgggccg
    15661 tccctacgag acgatgctcg ccggagcggc gatgtcgttc tcgctgctaa cttcttacac
    15721 gcgcgcacgc gcggagggtc tgggcgtgag gtgcgaggtc ggcctgctgg aacgcgccgg
    15781 acgggtgttg ttgctctccg tactctcgat cttcggcctc ctgaccgtgg gggtcgctct
    15841 cgtcgcggcc ggcgccctga ttacaaccgc ccagcgcatc ctgcacgtcc ggcgcgagac
    15901 ccggtcgtac ccggacacga gaagttgagc gcccgcgtga gcgcccgcgc gccccgcgac
    15961 gccgcgagcg attaccgggc gaagctctcc cacgaggctg cgcgcctcgc ccgcaagatg
    16021 cgggaggccg ggcatcagcc tccgctggga gatcccggct gcggtctcat ggtcgtcata
    16081 gaacagcccg tcgggccgcg tgtcctggac gctctctcgc gcagcctctc tgcgataggt
    16141 ctctctgacg cttacgtgac ggccgccggc acgggacgcc tgtacgaaga ggccttgtcc
    16201 acggagcctt cggcgctggt cgcggtcggg ccgggcgcgg cccaggggat agacgctctc
    16261 gaccacccgc tcgccctccg ctcctactcg gacgccacgc agggccagtg gttcgactgg
    16321 acgaagggca cggccgggct cctgctgcct ccactcgctc ccgccctgga cgacgaggaa
    16381 gccaagaggc ggttctggac gtcgttcctg gctctccgcc aactggctcc gcgcgccctc
    16441 tagccttgcc gcgcctcccg gccgcgcgtc tcttctgcat actgggactc gccgctctcg
    16501 ccgccttgac tgtatccctg ccgttcgcgg atctccaacc atctttcact gctcatgtgg
    16561 tttcgtacgc gctcgctctc gttcctgtgg cggctcttat ctacgggttc ccggatctcg
    16621 tgcgagatct cgccacaccc cggcttctga ctctcggcgc ggtctcggct accctgggct
    16681 ttctggccgc cctcgcgtgc cgccccgatg cattcgacgc cggaccacgc ttgcttccgc
    16741 tggctttact ctacctggct aatgtcttgc gcctgtgcgc cgccgcttcc ctgggtctag
    16801 ctctcgcccg ctacgtgatt tctcccggcg tggcgcttct catcgccgta ctggctgccg
    16861 tctcggacct cttcagcgtc ctggcgggac ccacgaaggt gctccttagc aagggctctc
    16921 cagcactgga ttttctgatc ctggtcttcc cctccttcgg cgcgcctctg ggtttcgggt
    16981 tcggactcag cgacttcgta tttctcgccc tcttcgccta tatcagcctc ctcctcggcc
    17041 tgcgttacca aaccacactc ctgttctgct ccgccgcggc tatgctcgcc gcggttacag
    17101 ccctctcact cgaacaaccc ctgcccgctc tcccattcat ctgcctcggg ttcgtgctcg
    17161 caaatatctg gcaaatcctc gccagaatgc gaaaataagc ctgctcagtc cccttctatc
    17221 cttacactga cgtaacgagt gttaagtttt attaaaaatt agtttcgtag aggaaacacg
    17281 caatcccttt atttaagcca tctttgtgaa tgagtaaggt tttcggcggc aaaatgggtg
    17341 ttgcgaggat gtttaggctt ccgtagagtg cccggcttag gaggggggat ttcgaaaatc
    17401 cccttcacaa cttgagatat gacagctacg gggaggagcc cggacgtatg cctaaaaggg
    17461 ccagtttcat tatgtttctc ggattcgtta ctgttatcgt atttgcgacc gggatctcgg
    17521 ccggagctgc gccccagggc gacacccaga gtaaacaggc ggaggttagt agcgcccagg
    17581 accggctgac cagtatccgt caggaggcca gttcttctta cgaggagtac aacaacgcgc
    17641 tcttccagtt aaacgagctc gacaaggagc tagacgcgac caatcaggac ctagagaagg
    17701 cccagagccg gctgaccgag gcccagagca acctcgaaga gcgggcttcc caggtctatc
    17761 gtagtgggaa tgttgggttc gtggacgtgc tggtcggggt agataacttc tccgacttcg
    17821 ctacccggtt ggatctgtgg atgaagcttc tcggccagga gcgcgcggag ttcgaaaccg
    17881 tgctagaggc gaagaacgac atcgcagcca agcaagcaag cctcgaagat cagcggtcgc
    17941 agcgcgcctc ggctctcaag gatgccgcgg agcggaagaa gcaggcggag aacgcccaaa
    18001 ccgaagcgca ggcgtatctg gattccctca acgcggatct ccaggccgcc atcgaagccg
    18061 atgctcagaa gcaggccgaa gcagcccgag ccgccgccga gaaggccgcc aagtccgagt
    18121 ccgccccgaa ggccgccaag tccgagtccg ccccgaaggc gcagccggag ttggtgcaga
    18181 tcgcagacgt gcagcctgcc gcccagcagg cggctccgca ggcatcgcgt gcagacctca
    18241 agagccagca gcaggccgcg gcccagcgtg ctgccgagcg cgccgccgcc cgcgccgccg
    18301 ccgagaaagc cgctgcggaa gccgagcgtc aggcaaaact cgcccagcag gaggctactc
    18361 agaagaaagc cgatcaacgc gccgctgccg agcagcgggc ggaggagaga caggccgccg
    18421 cccagcaggc ccgtgacgcc gccgaacagg ccgcgataga agaggccgct gc
//
LOCUS       DASSMB010000181        12263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_932261, whole genome shotgun sequence.
ACCESSION   DASSMB010000181 DASSMB010000000
VERSION     DASSMB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12263
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932261"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1080)
                     /locus_tag="VFJ72_10750"
     CDS             complement(73..1080)
                     /locus_tag="VFJ72_10750"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET7479979.1"
                     /translation="MVRQPAMRRLSILLELTVMCGLVFVWFPDIAYAQGHGHGGGVSL
                     SRYELLVGFAHAVTLGATVFLAGLVAFAALVWVPASRESGVDQTAASLLNRGAWAMFA
                     LLIVAGLIEVSLYAVRASGESFSLGLLLEAWFGTRVGHIWLARLGFALITVLVSAWAV
                     RERKTSYWWVAACLGAVLLMTLTQLSHAAAEGRLLPFAADWLHVIAASVWMGGLLGFP
                     LLLLGPLRAASPEIRAGTLGRTVRRFSKVATVAVSTIVLTGVYASLLHVPSLAALVSS
                     SYGRALVMKLGLAMMLLATGGINLIDKGQGPFDRMIAAELLMALGVFAATGFLSSLPP
                     P"
     gene            complement(1144..1740)
                     /locus_tag="VFJ72_10755"
     CDS             complement(1144..1740)
                     /locus_tag="VFJ72_10755"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HET7479980.1"
                     /translation="MTARNIVRWPAALLGLSVLWLLLLCGPALAHAGILKASPEYDED
                     VSKPPEQVRIRFNESVEAAFSPMEVFNQQGKRVDLDDANTDPDNSAVLVASLNPNLPS
                     GTYTVKWRVTSADGHPINGKYKFTVKSAGEAGESQPAGTREQEQAASQEKPKPAARGG
                     SGSGSSTANSVWLYSGIGLGALAVVALVGVALQRRGKP"
     gene            complement(1742..2206)
                     /locus_tag="VFJ72_10760"
     CDS             complement(1742..2206)
                     /locus_tag="VFJ72_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1775 domain-containing protein"
                     /protein_id="HET7479981.1"
                     /translation="MKERVEVPKGFEVTDVSSPSGWKGGIQGESLVWTGGEIAEDRVQ
                     EFTFTARTPDEPGEYAWRTFDTYKDGSVSKWIGPPDSEHPASVTRIGSGGSSGSGEME
                     GHEPSEGHHGAGMESGENIPDTGGMDPALLVSLTGFFTLALGLTGLAVLRRS"
     gene            complement(2500..3135)
                     /locus_tag="VFJ72_10765"
     CDS             complement(2500..3135)
                     /locus_tag="VFJ72_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479982.1"
                     /translation="MREATRARRVVLADDSALSRRAVRSLLKNEAGFEIVGEAGDGFE
                     ALQLARELGPDLILMDLDMPHCDGLLATRLIKRELPSSKVVILTVSDDAADLFEAIQN
                     GAQGYLLKSLAPADWLKCLRNLANDEAPPRELARRILAGFAAPVPVLEPSLTEREREV
                     LRLVAAAMTNRQVAEALYVSEQTVKNHLKSIMRKLRLKNRVELALYARRSA"
     gene            complement(3132..3974)
                     /locus_tag="VFJ72_10770"
     CDS             complement(3132..3974)
                     /locus_tag="VFJ72_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7479983.1"
                     /translation="MAVLLPTLCIGLFEFLRHQWLVGVLPMWLGHGWVGNVLGALVVA
                     AVVYFFVRVFAGMVGAAALEAARAREEAAVLTERQRIGREMHDGVAQALFYLRAKLNE
                     VGDLLAAGENERTLAELRAAEEELQETHRLVRAAISDLKRQPEIERFDEALRRTVSEL
                     AGRMALRLTCEAEAPCCALSAPYQRHLLAIVQEALTNAHRHGNARSASVKVGTSEETL
                     IVEVSDDGSGFDPDAVPQDGRYGLFIMEERARMIGGSMLLDSVPGLGTRILLRVPVRQ
                     PVAP"
     gene            complement(4213..4866)
                     /locus_tag="VFJ72_10775"
     CDS             complement(4213..4866)
                     /locus_tag="VFJ72_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7479984.1"
                     /translation="MTEPITVLLVDDHALVRQGVRAFLETQDDITVVAEASGGEEAVK
                     LAAEHAPDVALMDLIMPGMDGVEATRRLVARSPRTNVVMLTSYHDDEHVFPAIRAGAL
                     SYVLKEIGPDELADAVRKAASGEAVLHPRVAARVVRELHGARRTEPNVFHDLSDRELE
                     VLKLIAEGMSNAEIAGRLFVSEKTVKSHVSNILGKLHLADRTQAAVYAWREGVVRRD"
     gene            complement(4863..6356)
                     /locus_tag="VFJ72_10780"
     CDS             complement(4863..6356)
                     /locus_tag="VFJ72_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7479985.1"
                     /translation="MKSEIFALRRLRWKLTFSYTLVTVVALLALELILVSVLVAFIYS
                     PLLPRLVVQQIKDDLAPRLEKPLNESPPDIEGLRKELTFFAETSEVRGAGDNVGTGPV
                     SVRNLSPDEGSLFVVDDKRRLLVSVPEIRGFPEGERFDAGSIPGLEPLVDRAFDGEEN
                     SSRLSSNVNGRVVTVTPIEGKNGKVAGVLVGTLRLPNLTGPLLIAVGVSAVLLMIPAA
                     FLGALFGFVTAYGLTRRLQRLARAAQAWSRGDFSVTARDRSKDELGQLSRELNSMAAQ
                     LETLIQARGELATLETRNRFARDLHDSVKQQVFATSLQIAAARALISRDTNAAESHLS
                     QADELVRQAQKELNVLIAEMRPAALEGKGLAGALREYVDRWSQGAEIPAEFHVQGERA
                     SPLEVEQALFRVAQEALANVAKHSGATRAEVDLIYTPETLTLRIADDGAGFDTSQSPG
                     GGFGLQSMRERLARLGGSVSVESAPGKGTRVTGVCPLTDTAKKGGKP"
     gene            complement(6487..7236)
                     /locus_tag="VFJ72_10785"
     CDS             complement(6487..7236)
                     /locus_tag="VFJ72_10785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7479986.1"
                     /translation="MEATAMVREGGVWDEQHSVGKTIALHLVPGALITAFYAVAAPVV
                     RGFGFPSLMAIFLAIIFVLIPFELGYLFYAARRNGTSLGGAVRYREPVSRWQFVALVV
                     SLFVWAGIGTGLAVPLEDAIREDVFSFLPGWFLLQEDFSGYSTAALAITLVLGVIVNG
                     IAAPITEELYFRGYLLPRISRFGAWAPLMNMVLFSLYHFFTPWQNVGRIIGLLPMVYA
                     VWWKRSIYISMAVHVLGNLLSMAILAVVFFG"
     gene            7376..8239
                     /locus_tag="VFJ72_10790"
     CDS             7376..8239
                     /locus_tag="VFJ72_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HET7479987.1"
                     /translation="MREYRGFPTTKDHELLRTVTATRYVTPLREGGSLPAIVEADDLG
                     TYVLKFRGAGQGRKALIAELVAGEIGRCLGLPVPEIVLAHLDPDLGRAEPDPEIQDLL
                     KASSGLNLALDYLPGSLGFDPLVEPPGPELASRILWFDALVQNVDRTPRNTNLLVWHG
                     RLWLIDHGAALYFHHNWPGRESAGNGASLEAVAQRPYAAARDHVLLPFAGALQGADAA
                     LATVLTPEALRDVLALIPDAWISDEPGFGNAEEVRAAYLSYLTTRLREPRVWAHALEQ
                     ARDHIQEVGRG"
     gene            8232..8618
                     /locus_tag="VFJ72_10795"
     CDS             8232..8618
                     /locus_tag="VFJ72_10795"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HET7479988.1"
                     /translation="MASPFEYALLRVVPRVERGEFINAGVVLYCQDARFLEARIHLDP
                     ERLRALDPRLDPEKVLAHLEVAKRVCAGGPEAGSVGLLPPVQRFGWLVAPRSTVVQPS
                     PVHTGLAENPAEAIEHLLRTMVLAPE"
     gene            complement(8630..9217)
                     /locus_tag="VFJ72_10800"
     CDS             complement(8630..9217)
                     /locus_tag="VFJ72_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479989.1"
                     /translation="MMNYPLELSFKIVTLGTRVRVVDAAGAQVAYVRKKKFRLKEDIG
                     VYRDEGQKDLLFRLKADRVMDFGASYAVSGADGRPLGAVRQHGMRSIWKSHYGLTDAG
                     GSEVGYIHEENPWVKVLDGLMESLPFGDALGGLFFNPAYLAELRGDTVLRMKKERAVL
                     ESRFTVEKLGDFSDEDEALLLAGLIMTVILERDRG"
     gene            complement(9248..9487)
                     /locus_tag="VFJ72_10805"
     CDS             complement(9248..9487)
                     /locus_tag="VFJ72_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479990.1"
                     /translation="MLETRIEERPPSTARPGFGRSLTPGVREFLLDVLVSARSYDAPI
                     QEAEQDGDVELTNFLRELQRQDLARTGEITRLLRR"
     gene            complement(9605..10036)
                     /locus_tag="VFJ72_10810"
     CDS             complement(9605..10036)
                     /locus_tag="VFJ72_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET7479991.1"
                     /translation="MEAKNYGIAGEAKAEEQGPVTLMTQEERTWSVLSHLSIFLNLVT
                     GFLGPVAALIVYIVYRDRSQKVAFQALQAMWYQVAWIVILGAGWALTGLLTLVLIGFL
                     LIPVMALLTVVPFVHSAYAAYRVSKDGEYRYPIIADMIEAR"
     gene            10253..10879
                     /locus_tag="VFJ72_10815"
     CDS             10253..10879
                     /locus_tag="VFJ72_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7479992.1"
                     /translation="MTEGHRLFAALYDRFNGAMERGWMGERRARLLSGARGAVLEIGG
                     GTGANVPHYRDVERVMVSEPDPFMRRKLQPKLARARVPVEMSDAGAESLPFPDESFDT
                     VVSTLVLCTVPDQRAALAEIQRVLRPGGRLLFIEHVRGEGSVAVWQDRIEPLWRRLMG
                     GCHPNRDTVAAIRGSGFEIEALESFYPPVPLSGITPHVQGAATVRLAA"
     gene            10963..11421
                     /gene="lspA"
                     /locus_tag="VFJ72_10820"
     CDS             10963..11421
                     /gene="lspA"
                     /locus_tag="VFJ72_10820"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7479993.1"
                     /translation="MSRRAGLFLALGLAVAVFFVDQGIKSVVEGSMRVGESITLVSGF
                     LSLTYIKNDGGAFGILGGSQTVLLIGSAFAVAVVLWMLLAGEPSKLTTLGCGLILGGA
                     AGNLLDRLSSGEVTDYVHFSFWYIFNAADAAIVIGVALLLLSALRPAPKG"
     gene            complement(11424..11726)
                     /locus_tag="VFJ72_10825"
     CDS             complement(11424..11726)
                     /locus_tag="VFJ72_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HET7479994.1"
                     /translation="MIKLTVLYGHPGDPDAFDEYYEKEHLPLAAKIPDVQRFESGRVG
                     AVDGTEPPYYRIAELWFESAERMGEALSSPEGEAATGDIPNFATGGATVFVSEVEA"
     gene            complement(11859..>12263)
                     /locus_tag="VFJ72_10830"
     CDS             complement(11859..>12263)
                     /locus_tag="VFJ72_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family zinc transporter"
                     /protein_id="HET7479995.1"
                     /translation="ALMDGIPESAAIGISLLGGGKISAALVAAVFLSNVPEGLSSASG
                     MKRAGRSTAHIMGLWAGVTVASAVAALLGYLFLAGASENVVAAIQSFAAGAILTMLAS
                     TMMPEAYEDGGAFVGLVTTIGFLLAFVLSHLE"
BASE COUNT         2173 a         4193 c         3780 g         2117 t
ORIGIN      
        1 atagtggttg ctatctgggt gctgctggtg atcgcggcgt tcatgctcgt gcggcgctac
       61 ttctgatccg ggctatggcg gaggcaggct gctaaggaaa cccgtagccg cgaacacgcc
      121 cagtgccatc agcagctcgg cggcgatcat gcggtcgaac ggtccctggc ccttgtcgat
      181 gaggtttatg ccgccggtcg cgagcagcat catcgccaga ccaagcttca tgacgagagc
      241 gcggccatat gagctgctga ccagggcagc caggctcggt acgtgtagca gactcgcgta
      301 cactcccgtg aggacgattg tcgagacagc gacggtggca accttcgaga agcgccgcac
      361 ggtccggccc agtgtcccgg ccctgatttc gggcgacgcc gcgcgcagcg gcccgagtag
      421 cagtaacggg aaccctagca gtcctcccat ccagacggat gcggcaatga cgtgaagcca
      481 gtcggcggcg aaaggcagta agcgtccctc ggcggcggca tgactcaatt gagtcagggt
      541 catgagcagc acagccccaa gacaagccgc tacccaccag taggatgttt ttctctcacg
      601 aacagcccag gccgatacga gaaccgtgat gagagcgaac cccagccgcg ccagccagat
      661 gtgccccacc cgcgtcccga accaggcctc caggagcagc ccgaggctga aggactcacc
      721 ggaggcgcgc accgcataca gagaaacctc gatcagaccc gccactatta gcagggcgaa
      781 catggcccac gccccccggt tgagcaatga ggcggcggtc tgatctacac cggactcccg
      841 actggccggg acccacacca gtgccgcgaa agccacgagc ccggccagga acacggttgc
      901 cccgagcgtc acggcatgcg caaagccaac caacaactcg tacctgctga gggatactcc
      961 tccaccgtgc ccgtgtcctt gcgcgtaggc aatgtccggg aaccacacga acaccagccc
     1021 gcacatcacg gtcagctcca ggaggataga gagtcgccgc atagccggtt gtctcaccat
     1081 gctgctattc atatcttagc gtttcgggcc ggcgcgacag ctccgacact aagatccgcc
     1141 ccatcaaggt ttaccccggc gttgcagcgc aacccctacc agcgccacga cggccagagc
     1201 gccgaggccg ataccgctgt agagccagac gctgttggcg gtgctggatc cactgcctga
     1261 acctccacgc gcggcgggct tgggtttctc ttgagaagcg gcttgctcct gctcgcgggt
     1321 tccggccggc tgcgattctc cggcctctcc cgcgcttttc acggtgaact tgtatttccc
     1381 attgatggga tgcccgtcgg ccgacgtcac gcgccacttc accgtatagg tgccactagg
     1441 gaggttcggg ttgagactgg ctaccagaac ggccgaatta tcggggtctg tgttcgcatc
     1501 gtccaggtcc acacgcttgc cctgttggtt aaaaacctcc atgggactga aggccgcctc
     1561 cacggattcg ttgaaccgaa tcctcacctg ctcaggaggc tttgagacgt cttcatcgta
     1621 ctcgggagac gccttcagta tgcccgcgtg ggcaagggcc gggccgcaca agaggagcag
     1681 ccagaggacc gacagcccca gaagagctgc cggccaccgg acgatatttc ttgccgtcat
     1741 atcaggatct ccgcagtacc gccagtccgg tcaatccgag cgccagagtg aagaatcccg
     1801 tgaggctgac gagaagcgcc gggtccattc cacccgtgtc gggtatattc tcgccgctct
     1861 ccatcccggc gccgtggtgg ccttcgctgg gctcatggcc ttccatctcc ccggaaccgc
     1921 tcgatccgcc cgagccgatc cgtgtcacgg acgcagggtg ttcggaatcg ggcgggccga
     1981 tccacttcga cacgctgccg tccttgtagg tgtcgaacgt cctccaggca tattcccccg
     2041 gctcatcagg agtgcgcgcc gtaaaagtga actcctgcac cctatcttca gcgatctctc
     2101 cgccagtcca gacgagggat tcaccctgga tcccaccctt ccagccggaa ggcgagctta
     2161 catccgtcac ctcgaagccc ttcggcacct cgactcgttc cttcacgatg gggacgcttt
     2221 tttctccggg tgcggaaacg gtgaacgtct cggtgctgcc tggagatgcc tcgctcggcg
     2281 agacttcggt gtgcgcccag gcaaccccgg tcacagtaag tgttatcaag gcgcttagag
     2341 ccgttactcg gagcactgtc ttgagcatac ggtatccttt ccaaaacacc cacacttgca
     2401 ttctcgaccg tagtctagac tgacttcacg ggacggttat atagcccttt ggggctatat
     2461 agaaaggtaa aaagggtaac tcgcccgtgg tggaacgctt caagcggagc gcctggcgta
     2521 gagagcgagc tccacgcggt tcttcaggcg cagcttcctc attatgctct tgaggtggtt
     2581 ctttaccgtc tgctccgaga cgtagagcgc ctcggcaacc tgccggttgg tcatggcggc
     2641 ggctacgagc cggagcacct cccgttcgcg ctccgtcagg ctcggctcca gcaccggtac
     2701 cggcgcggcg aacccggcaa gtatgcggcg ggccagttcc cgtggcggag cctcatcgtt
     2761 tgccaggttt cgcagacact tcaaccagtc cgccggcgcg aggctcttga gcaggtagcc
     2821 ctgcgcgccg ttctggatcg cctcgaagag atccgccgcg tcgtcggaga ccgtcaggat
     2881 gaccaccttt gaggagggga gttcgcgctt gatgagcctc gtcgccagaa ggccgtcgca
     2941 atgcggcatg tctaggtcca tcaagatcag gtccggcccc agctcgcgcg cgagctggag
     3001 cgcctcgaac ccgtctccgg cctccccgac gatctcgaac cctgcctcat tctttaacag
     3061 ggagcgtacc gcccggcggg agagcgcgct gtcgtcggcc aggaccaccc gccgcgctct
     3121 ggtagcctcc ctcacggcgc aaccggctgt cgaaccggaa cgcgcagcag gatgcgggtc
     3181 ccgagccctg gcaccgagtc gagcagcatg ctgccgccga tcatccgcgc ccgttcctcc
     3241 attatgaaaa gcccatagcg tccgtcctgc gggaccgcat ctgggtcgaa cccgctcccg
     3301 tcatccgaga cctcgactat cagggtctcc tcagaggtcc cgaccttcac gctggccgac
     3361 cgggcgttac catgccggtg cgcgttggtg agggcctcct gaacgatagc cagcagatgc
     3421 ctctgatacg gagcggagag cgcgcagcaa ggtgcctctg cctcgcaggt cagccgcagg
     3481 gccatgcgtc cggccagctc ggacaccgtg cgccggagcg cctcatcgaa acgctctatc
     3541 tccggctgcc gtttgaggtc cgagatcgcc gcgcgcacca gccggtgggt ctcctgcagc
     3601 tcctcctccg ccgccctcag ctctgcgagg gtccgctcgt tctcgccggc ggccagaagg
     3661 tcgcccacct cgttcagctt ggcccgcagg tagaacaggg cctgtgcgac gccgtcgtgc
     3721 atctcgcgtc cgatgcgctg tcgttccgtg agcacggcgg cctcctcgcg ggcccgagcc
     3781 gcttccaggg ctgccgctcc gaccataccg gcgaagaccc ggacgaaaaa gtacaccaca
     3841 gccgcgacca ccagggcgcc caggacgttc cccacccagc catgccccag ccacattggc
     3901 agcacgccaa ccaaccactg gtggcgcaga aactcaaaga ggccgatgca cagcgtgggc
     3961 aacagaaccg ccatccaccg gagtctcccc aggtacgcgg tttcttcctc ctccccggca
     4021 acggacaccg cgcgaagacg ctcccccatc accggcagat tcaatctccg aagcagcatc
     4081 cgctctcgca cctccgtcgc gcgggatctc ccaaaaccca gatacggacg atgatagcct
     4141 aaccagccgc ggcgggtatc ctgcggatgt actacatttg aatagcccaa acgggctacg
     4201 ttcgcgcgcc gttcagtcgc gccggacgac gccctcgcgc caggcgtaga cggcggcctg
     4261 cgtcctgtcc gccaggtgca gcttgccgag gatattcgag acgtggctct tcaccgtctt
     4321 ctcgctcacg aagaggcggc ccgcgatctc cgcgttgctc atcccttccg ctattagttt
     4381 caagacctca agctcccggt cggagaggtc gtggaagacg ttaggttcgg tgcggcgggc
     4441 gccgtgcagc tcgcgtacga cgcgggcggc gacgcgagga tggagcaccg cttccccgga
     4501 tgcggcctta cggacggcgt cggccagctc gtccggtccg atctctttca ggacgtagga
     4561 gagagcgccg gcccggatcg ccgggaagac gtgctcatcg tcgtggtagg aggtgagcat
     4621 cacgacgttt gtgcgcggag accgggccac gaggcgtcgg gtagcctcca cgccgtccat
     4681 gcccggcatg atcaggtcca tcagcgccac gtcgggggcg tgctcggcgg cgagcttcac
     4741 tgcctcttcc ccaccactag cctccgccac cacagtgatg tcgtcctgcg tctcaaggaa
     4801 cgcccgcacc ccctgacgca ccagcgcgtg gtcgtccacc agcaggaccg ttataggttc
     4861 ggtcatggct tgccgccttt cttcgcagtg tccgttagcg gacagacgcc ggttacccgc
     4921 gtgcctttcc ccggcgcgct ctcgacactg acgctgccac cgagcctcgc cagccgctcg
     4981 cgcatgctct ggagtccgaa gcccccgccg gggctctggg aggtatcgaa gccggctccg
     5041 tcgtcggcga tgcggagggt gagagtttca ggggtataga tcaggtctac ttcagcgcgc
     5101 gtcgcgccgc tgtgcttcgc cacgttcgcc agggcttcct gcgccacccg gaagagtgcc
     5161 tgctcgacct ccagcggcga cgcgcgctcg ccctggacgt ggaactcggc cggtatctcc
     5221 gccccctggg accatcggtc gacgtactcg cgcagggcgc ccgccagccc cttaccttca
     5281 agagcggcgg gacgcatctc ggctatcagg acgttcagtt ccttctgcgc ctgccggacc
     5341 agttcgtcgg cctgtgaaag gtgagattcg gcagcgttgg tgtcgcggga gatcagggct
     5401 cgagcggcgg cgatctgcaa ggaggtggcg aagacctgct gttttacgga gtcgtgcagg
     5461 tcgcgggcga agcggttgcg ggtctccagc gtggcaagct ctcccctggc ctgaatgagc
     5521 gtctccagtt gcgcggccat gctgttcaac tcccgcgaga gctggcccag ctcgtccttc
     5581 gagcggtccc ttgcggtaac ggagaagtcg ccccggctcc acgcctgggc agcacgagcg
     5641 agccgttgca gacgcctcgt caggccatac gcggtgacga agccgaagag cgcgccgagg
     5701 aaggcggccg gtatcatcag caacacagcg ctgacgccga ccgcgatcag gagcggtccg
     5761 gtgaggttcg gtagccgcag agtccccacg agcaccccgg caaccttccc attcttcccc
     5821 tcgatgggcg taaccgtcac caccctgccg ttcacgttcg acgagaggcg gctggagttc
     5881 tcttcgccgt cgaaggcccg gtccacgagt ggctccagtc cggggatgct tccggcgtcg
     5941 aagcgctctc cctcggggaa ccctcttatc tcgggcacgg agaccagcag gcggcgctta
     6001 tcgtccacca cgaacagaga accttcatcc gggctcaggt tgcggacgct cacgggacct
     6061 gtcccgacat tatcaccggc tcccctgact tcggaagtct cggcgaagaa cgtcaactcc
     6121 ttgcggagac cttcgatgtc gggcgggctc tcgttgagcg gcttctccag gcgcggcgcg
     6181 aggtcgtctt tgatctgctg cacgaccagc ctcggcagga ggggcgagta gataaaagcg
     6241 acgagcacgc tgaccaggat caactccaga gcgagcaacg ccaccacggt aaccagcgtg
     6301 tagctgaatg tcagcttcca ccgcagccgc cgcagcgcga agatctcgct cttcatcggt
     6361 cgtagccctc tctcatggca ggctcagtct agtcccgtgc gccccggagc gcatcggcct
     6421 ccggtcgtga agcgcctcgg tccagaggac cagtaagccg gatctttctc tcccgcgagc
     6481 gtaacgttac ccgaagaaca ccaccgccag gatcgccatt gagagcaggt tccccagcac
     6541 gtgcacggcc atgctgatgt agatactcct cttccaccac actgcgtaga ccatcggtag
     6601 caggccgatg atgcgcccga cgttctgcca gggcgtgaag aagtggtaga gcgagaatag
     6661 gaccatgttc attagaggtg cccacgctcc gaaacgcgag atcctgggca gcagataccc
     6721 gcggaagtaa agctcctccg tgatgggagc ggcgatacca ttcactatta caccgagtac
     6781 cagcgtgatc gccagcgccg ccgtcgagta cccggagaag tcctcctgca aaaggaacca
     6841 acccggcagg aaggagaaca cgtcctccct gatggcatcc tccagcggaa ccgcgaggcc
     6901 cgtcccgatc ccggcccaca cgaagagcga gacgaccagc gcaacgaact gccaccgcga
     6961 gaccggctcc cggtagcgca cggcccctcc gagagaagtc ccgttcctcc tcgccgcgta
     7021 gaacaggtaa cccagctcga acgggatgag gacgaagatt atggcgagga agatcgccat
     7081 gagcgacggg aacccgaacc ctctcaccac cggagccgca acggcgtaga acgccgtgat
     7141 cagggctccc ggcacgaggt gcagcgcgat cgtcttaccg acggagtgct gctcatccca
     7201 aacaccgcct tctcgcacca tcgccgtggc ttccatccct tctctcgacg cccgccgtac
     7261 gagaacaatc tacagtcgcg cgggtccggg agcatcggtc cggaggatga atgttacccg
     7321 tacgtcagga ccatattgtc gccaccacac agcctgttgt agaggcggcg ggcgggtgcg
     7381 agaatatcgc gggttcccga ccacgaagga ccatgagttg ctccgcaccg tgaccgccac
     7441 acgctacgtt acgcccctgc gcgagggagg ctctctgccg gccatcgtgg aggccgacga
     7501 cctggggacc tacgtcctga agttccgggg cgctggacag ggacgcaagg cgctcatagc
     7561 ggagcttgtg gccggtgaga taggccgctg tctggggttg ccggtaccgg agatcgtgct
     7621 cgcacatctc gacccggatc tcggccgcgc ggagccggac cccgagatac aggacctgct
     7681 gaaagccagc tccggcctga acctggccct ggattacctg cccggctctc tgggcttcga
     7741 cccgctcgtg gagccgccgg ggccggagtt ggcctcacgc atcctgtggt tcgacgccct
     7801 ggtgcagaat gtggaccgga ccccgcgcaa cactaacctt ctcgtctggc acggcaggct
     7861 ctggcttata gaccacggcg cggcgctgta cttccaccac aactggcccg gccgggagtc
     7921 tgccgggaac ggagcctccc tcgaagccgt ggcccagcgt ccctacgcgg cggccagaga
     7981 ccacgtactg ctccccttcg ccggtgccct acagggcgcg gacgcggcgc tcgccaccgt
     8041 gctcacgccg gaggcgctgc gagacgtgct ggccttgatc ccggacgcct ggatctcgga
     8101 cgagcccggc ttcggaaacg cggaggaggt ccgggccgcc tacctctcct acctcaccac
     8161 gcgcctgcgg gagccgcgag tctgggcgca cgcgctggag caggcacggg atcatatcca
     8221 ggaagtagga cgtggctagc ccgttcgagt acgccctcct gcgggtggtc ccgcgcgtgg
     8281 agcgcgggga gttcatcaac gcgggcgtcg tgctctactg ccaggacgcg cgctttttgg
     8341 aggcccgcat ccacctcgac cccgaacgcc tgcgcgcgct cgacccgcgc ctggacccgg
     8401 agaaagtcct cgcccatctg gaggtggcga aacgggtctg cgcgggcggc cccgaggcgg
     8461 gctccgtggg gctcctgccg ccggtgcagc gcttcgggtg gctggtcgcc ccgcgcagca
     8521 ccgtcgtcca gccctccccg gtacacaccg gccttgccga aaacccggcg gaggcaatcg
     8581 agcacctcct gcggacgatg gtcctcgctc cggagtaagg gtcctggatc tagccgcggt
     8641 cccgttcgag gatgaccgtc atgatcaggc cggccagtag cagcgcctcg tcctcatcgg
     8701 agaagtcgcc gagcttctct acggtgaacc tgctctccag caccgcccgc tccttcttca
     8761 tccgtaagac cgtatcgccg cgcagctccg ccagataggc ggggttgaag aagagaccgc
     8821 cgagagcgtc gccaaacggg agggactcca tcagaccgtc gaggaccttg acccaggggt
     8881 tctcctcgtg gatgtaaccg acctccgatc cgcccgcgtc ggtgaggccg tagtggctct
     8941 tccagatgga gcgcatcccg tgctgccgga ccgcgccgag cggtcgtccg tccgcgccgc
     9001 tcaccgcata gctcgccccg aagtccatca cccggtcggc cttcaggcgg aagagcaggt
     9061 ccttctggcc ctcgtcccgg tagacgccga tatcctcctt cagtcggaac ttcttcttgc
     9121 ggacgtaggc gacctgggcg cccgccgcgt ccaccacccg cacccgcgtg ccgagggtca
     9181 cgatcttgaa gctcaactcc agtgggtagt tcatcatgtc tcatcctcct ggctgggtgc
     9241 gtcctactta ccgccgcagc agtcgcgtga tctctccggt cctggcgagg tcctgacgct
     9301 gcaactcgcg cagaaaattg gtcaactcca cgtctccgtc ctgctcggcc tcctggatag
     9361 gcgcgtcgta agaccgggcg ctgaccagca cgtccagcag aaactccctg acgcccggcg
     9421 tcaagctgcg cccgaagccg gggcgcgcgg tactcggggg ccgttcctcg atcctggtct
     9481 caagcaccag cttctccttc cctgtatcac attccgtact actagatacg cgcgagacgc
     9541 tgcaaagttg tctcgcgcaa aagacgacgc ccccggttcg accgggagcg tcgtgaggtt
     9601 acgcttagcg ggcttcgatc atgtccgcga taatcgggta gcggtactcg ccgtccttgc
     9661 tcacccggta agccgcgtac gcgctgtgca cgaacggcac caccgtgaga agggccatca
     9721 ccgggatcag gaggaacccg atcaagacca gcgtcaacag tcccgtgagg gcccatccgg
     9781 cgcccaggat cacgatccac gcgacctgat accacatcgc ctgcaacgcc tggaacgcca
     9841 ccttctgcga gcggtcccgg tagactatat agacgatcaa ggccgccacc ggcccgagaa
     9901 acccggtgac caggttgagg aagatgctca ggtgcgagag caccgaccag gtcctctcct
     9961 cctgcgtcat cagcgtcacc ggaccctgct cctccgcttt tgcctcgccc gcaattccgt
    10021 agttctttgc ttccacgact gctcctctct gttgctcaac aacctacgct ataaagttac
    10081 gcccggacct cccttccggc atcggaccac ggaccgaata caagtaggtc ttcgggacca
    10141 ggttttcgcg tgcgcgctca ggccatccgt ggcaaactca atgcaggttc atgagaacgt
    10201 tcgtaagaga tatcgagacc agcacccgcg tcccgtcgtg gaggatcgca ccatgaccga
    10261 aggccatcgc ctgttcgccg ccctctacga ccggttcaat ggggcgatgg agcgcggctg
    10321 gatgggcgag cgacgcgccc ggctgctctc cggggcgcgg ggcgcggtgc tggagatagg
    10381 cggcggaacc ggcgcgaacg tcccccacta ccgcgatgtc gagcgggtca tggtctccga
    10441 gccggacccg ttcatgagga ggaagctgca accgaagctc gctcgtgctc gcgtacccgt
    10501 cgagatgtcc gatgcgggcg cggagtcgtt gcctttcccg gacgagagct tcgacacggt
    10561 ggtctcgacg ctcgtcctgt gtaccgtccc ggaccagagg gcggcgctcg cggagatcca
    10621 gcgcgtgctc cgtcccggcg gaaggctgct gtttatcgag cacgtgcgcg gcgagggctc
    10681 cgtcgccgtg tggcaggacc gcatcgaacc gctgtggaga cgcctaatgg gcggctgcca
    10741 cccgaaccgc gataccgtcg ccgctatccg gggatcgggg ttcgagatcg aagcgttgga
    10801 gagcttctac ccgccggtgc cgctctccgg cataaccccg cacgtccagg gcgcggcgac
    10861 cgtccggctc gccgcctgag aaggtcgtag cgcgtatgct aggatcaacg ctccgatgaa
    10921 cctcgcgtcc gaacaacatc cagacctccg tcggagagag cgttgagccg cagggcgggt
    10981 ctctttctgg ccctcggact ggccgtagcg gtcttcttcg tggaccaggg gataaagtcc
    11041 gttgtggagg gctcgatgcg ggtcggggag agcataacgc tcgtgtccgg cttcctgagt
    11101 ctcacgtaca taaagaacga cggcggagcg ttcggaatcc tcggcggcag ccagaccgtg
    11161 ctcctgatcg ggagcgcctt cgccgtcgct gtagtcctct ggatgctcct cgcgggagag
    11221 ccctcgaagc taaccacgct cggctgcggc ctgatcctgg gcggagccgc cggcaacctc
    11281 ctcgaccgcc tgagcagcgg agaggtcacc gactacgtcc atttctcatt ctggtacatc
    11341 ttcaacgccg ccgacgccgc gatagtgata ggcgtggcac tactgctgct ctccgcgctc
    11401 cgccccgcgc cgaagggctg agactaggct tccacctcag agacgaagac cgtcgcgccg
    11461 ccggtggcga agttggggat gtcgccggtc gcggcctcgc cttccggtga ggagagggcc
    11521 tcccccatcc tttccgcgct ctcgaaccag agttcggcga tgcgatagta aggcggctcg
    11581 gtgccgtcta ccgcgccgac cctacccgac tcgaagcgct ggacgtcggg gatctttgcc
    11641 gcgagcggca ggtgctcttt ctcgtagtac tcatcgaaag cgtccgggtc gccgggatgc
    11701 ccgtacagta cagtcagctt gatcatgcct ctcctcctcg tcgccgcagt ttcgccttac
    11761 tggcccgtct tgacgaaccg ttccccaggt tcccgagtat tctacagcgg tggcgaaccc
    11821 gaagcgcgat tcggaacgcc gtgcggagcg ccacagcctt actccagatg gctcaggacg
    11881 aacgcgagga ggaacccgat ggtcgtgacg aggccgacga acgctccccc gtcctcgtac
    11941 gcctccggca tcatggtgct ggcgagcatg gtcaggatgg ccccggcggc gaaagactgg
    12001 atggcggcga cgacgttctc cgaggcgcca gcgaggaaga ggtagccaag aagcgcggcc
    12061 accgccgagg caaccgtaac gccagcccag aggcccatga tgtgggccgt cgagcggccc
    12121 gcccgcttca tcccggaggc cgaggagaga ccttccggga cgttggagag gaagacggcg
    12181 gcgacgaggg cggcactgat cttgccgccc cccagcagac ttatcccgat ggccgccgac
    12241 tccgggatgc cgtccatgag cgc
//
LOCUS       DASSMB010000182         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_552887, whole genome shotgun sequence.
ACCESSION   DASSMB010000182 DASSMB010000000
VERSION     DASSMB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552887"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFJ72_10835"
     CDS             <1..311
                     /locus_tag="VFJ72_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7479996.1"
                     /translation="MALVGVWKPGISGVWAVLIGFGGLPALFFLSHMVDTARSAANPY
                     CNNVGPNAGEITIPPGVRMVECSSIPASYYVMFAVFAGIMLLGVFLGFLRRARSHSTA
                     "
     gene            complement(349..780)
                     /locus_tag="VFJ72_10840"
     CDS             complement(349..780)
                     /locus_tag="VFJ72_10840"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET7479997.1"
                     /translation="MDRKSRIELLVDHVRDPRHQGVLDDADVVMPGGSPECGGSVVVY
                     LKGDGNGGIEALSWTGQGDTISMGATSIIMERVLAEGMDLDQALSLDYDELVERLGRD
                     VIGSRTRNATLGLSTLKNAIHKYRRDRRSANLATEPVRSGG"
     gene            complement(789..1664)
                     /locus_tag="VFJ72_10845"
     CDS             complement(789..1664)
                     /locus_tag="VFJ72_10845"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7479998.1"
                     /translation="MTEQQIAEKGYAHPEALVSTDWVAEHLNDDNIRIVESDEDVLLY
                     DIGHVPNAVKIDWVEDLNDPLVRDYLDPEHFARLMGEKGITPDTTVVFYGDKNNWWAT
                     YALWVFRLFGHDNVKVMDGGRIKWEAEGREMTQEVPSFPQTDYPVPVRDDAKIRAFKE
                     DVLKHLEKVGSGGSLIDVRSPGEYSGELLHMPDYPQEGALRGGHIPGAANVPWARAVR
                     EDGTFKSADELREIYEGEAGLNEGDDVIAYCRIGERSSHTWFVLNYLLGYDQVRNYDG
                     SWTEWGNSVRAPIER"
     gene            1815..>3125
                     /gene="cofG"
                     /locus_tag="VFJ72_10850"
     CDS             1815..>3125
                     /gene="cofG"
                     /locus_tag="VFJ72_10850"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HET7479999.1"
                     /translation="MIEFAVAGDLDRERAVELARLVYEDPAPAMRAAAGLRDRTFGPR
                     VTYSRKVFIPLTKLCRDNCGYCTFAHPPRPGERAYLTPEEVLEIARAGAEAGCKEALF
                     TLGDKPEKRYPEARRELREMGFGTTIEYLVHCCRLVLEETGLLPHANPGVLSEDEVRA
                     LREVSVSQGIMVEQVSERLLGRDLAHWASPDKVPARRLATLDAAGRLAVPFTTGMLIG
                     IGETVEERVDTLLAIREQHERHEHIQECIVQNFRAKPGTRMAHAPEPSESEMLASIAL
                     ARLLLPPDVSVQAPPNLAGTEDGDRSYARYIDAGIDDWGGVSPVTPDHVNPERPWPHL
                     EELEQATQEKGYLLLERLAVRPAFAVRAERWVDERLRSQVWAGIDAEGFARVESWAPG
                     TIEPVPEKTVAEARGGRPSKVRGEFARALSGAGERDLSEEEIALL"
BASE COUNT          512 a         1030 c         1024 g          559 t
ORIGIN      
        1 ctatggcgct tgtcggggtc tggaagccgg gaattagcgg agtgtgggcc gtcctgatcg
       61 gttttggagg acttccagcg ctgttcttcc tgtcgcacat ggtcgatacg gccagaagcg
      121 cagcaaaccc ctactgtaac aacgtgggtc ccaacgctgg agaaataact atcccgcccg
      181 gcgtgcgcat ggtcgagtgc tcatccattc ccgccagcta ctacgtcatg ttcgcggtct
      241 ttgccgggat catgctcctc ggcgtctttc tgggattctt gcgacgcgcc cgctctcact
      301 cgaccgcctg acatcagggc gcgttatgtg agagatccgt gccgcaggct atccgccgct
      361 gcggacaggc tctgttgcca ggttggcgga gcgccggtcc cggcggtact tgtgaatggc
      421 gttctttagc gtggaaagcc cgagggtggc gttgcgggtg cggctgccga tcacgtcgcg
      481 gcccagcctc tccacaagct cgtcatagtc cagggacaga gcctgatcca ggtccattcc
      541 ttcggccagc acacgctcca ttatgattga cgtggcgccc atgctgatcg tatctccctg
      601 tccggtccac gagagggctt ctatcccgcc gttgccgtcg cccttgaggt agacgaccac
      661 cgatcccccg cactcggggc taccgccggg catgacgacg tcggcgtcgt caaggacccc
      721 ctggtggcgg gggtccctga cgtggtccac gagcagctct atacggctct tgcggtccaa
      781 agggctcttt acctctcgat gggggcgcgc acggagttgc cccactcggt ccaggagccg
      841 tcgtagttgc gcacctgatc gtagccgagg aggtagttca ggacgaacca cgtgtggctg
      901 gagcgctccc cgatgcggca gtaggcgatc acgtcgtcgc cctcattcag gccggcctcg
      961 ccctcgtaga tctcgcgcag ctcgtccgcg ctcttgaagg tcccatcctc cctgacggcc
     1021 cgtgcccacg gcacgttggc agcacccgga atgtgcccgc cgcgaagcgc gccctcctgc
     1081 gggtagtccg gcatgtgcaa cagctcgccc gagtactcgc cggggctccg cacgtcgatc
     1141 agggacccgc cggagccgac cttctcgagg tgcttgagca cgtcctcctt gaaggcccgg
     1201 atcttcgcgt cgtccctgac cgggaccggg tagtcggtct gcgggaagct cgggacctcc
     1261 tgggtcatct cgcggccctc ggcctcccac ttgatgcgcc cgccgtccat gacctttaca
     1321 ttgtcgtggc cgaagagccg gaagacccac agcgcgtagg tcgcccacca gttgttcttg
     1381 tcgccgtaga acacgaccgt ggtgtccggc gtgatgccct tctcgcccat cagccgcgcg
     1441 aagtgctcgg ggtccaggta gtcgcgcacc agaggatcgt tcaggtcctc gacccagtcg
     1501 atcttgaccg cattcgggac gtgcccgatg tcgtagagca agacgtcctc gtcactctcc
     1561 acgatgcgga tattgtcgtc gttcaggtgc tcggctaccc agtcggtgct aaccagcgcc
     1621 tcgggatgcg cgtaaccctt ctccgctatc tgctgttccg tcacggtttc tcctcttctc
     1681 cgttttcaac cgcacaggcc gatattctag cgtataagtc catctatttc cgaccaaaat
     1741 tatcgggttt aacaaaaacg ccggatgcag cggttatact ggccggcatg atgggacaga
     1801 gaggttacga ggcgttgatc gaatttgcag tcgcggggga tcttgaccgc gagcgggccg
     1861 ttgagctggc gcgtttggtt tacgaagatc cggcccccgc aatgcgggcc gcggccgggc
     1921 tacgggaccg gacgttcgga cccagggtca cctactcccg caaggtcttt atcccgctga
     1981 cgaagctctg ccgcgacaac tgcggctact gcaccttcgc ccaccccccg cgtcccggcg
     2041 aacgcgccta tctcacaccc gaggaggtgc tggagatagc gcgggcgggc gcggaggccg
     2101 gatgcaagga ggcgctcttc acgctcgggg acaagccgga gaagcgctat ccggaagcgc
     2161 gcagggagct tcgagaaatg ggcttcggga cgacgatcga gtacctggtc cactgctgcc
     2221 ggctcgtgct ggaggagacg ggcctgctgc cgcacgccaa ccccggcgtc ctctcagagg
     2281 acgaggtgcg ggcgctgcgg gaggtatccg tgtcgcaggg gatcatggtg gagcaggtct
     2341 cggaacggct actgggccgc gacctcgccc actgggcctc gccggacaag gtgccggcgc
     2401 gcaggctcgc gacgctggac gccgcgggca ggctcgcggt gccgttcacg acggggatgc
     2461 tcatcgggat cggggagacg gtcgaggagc gggtggatac cctgctggct atccgggagc
     2521 agcacgagcg ccatgaacac atccaggagt gcatcgtcca gaacttccgg gccaagccag
     2581 gcaccaggat ggcccacgcg ccggagccct cggagagcga gatgctcgcc agcatcgccc
     2641 tcgcccggtt gctcctcccg ccggacgtga gcgtgcaggc accaccgaac ctcgcaggga
     2701 ccgaggatgg cgaccgctcc tacgcccgct acatagacgc cggcatagac gactggggcg
     2761 gcgtctcacc cgtcacgccg gaccacgtca accccgagcg cccctggccg cacctcgaag
     2821 aactagagca ggccacgcag gagaaaggct acctgctgct ggagcgactc gcggtgcgcc
     2881 cggccttcgc cgtccgcgcc gaacggtggg tggacgagag gctgcgctct caggtgtggg
     2941 ccggaatcga cgccgagggg ttcgcgcggg tcgaaagttg ggcaccaggg acgatcgagc
     3001 ccgtgccgga gaagaccgtc gccgaagccc gcggcgggcg gccctcgaag gtgcggggcg
     3061 agttcgcgcg ggcgctctcc ggggccgggg aacgcgacct ctcagaagag gagatagcgc
     3121 tgctc
//
LOCUS       DASSMB010000183         9589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_932889, whole genome shotgun sequence.
ACCESSION   DASSMB010000183 DASSMB010000000
VERSION     DASSMB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932889"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFJ72_10855"
     CDS             <1..438
                     /locus_tag="VFJ72_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HET7480000.1"
                     /translation="EALSANEPMREIVRRFASEGRPIVAECGGLLYLARELDGHPMCG
                     VLDTAARMTERLTLGYREARAPVDSVLAAEGVTVRGHEFHYSVVEPGFGESPAWELTG
                     RGAEGFVAGGMHASYLHTHWAATPELPRRFVRAATRSAVEVRA"
     gene            435..1148
                     /gene="cobI"
                     /locus_tag="VFJ72_10860"
     CDS             435..1148
                     /gene="cobI"
                     /locus_tag="VFJ72_10860"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HET7480001.1"
                     /translation="MSGRLVGIGVGPGDPELLTIKGLRALREADEVFVPVADTGEVGR
                     AEATVREYLEPGRVRRLLFALSDDAEARERNWTNAAREVAEALRGGKVCAFATIGDPN
                     VYSTFTYLARTVRESMPEVEVQTVPGITAMQDLASRSGTVLLEGDERLALLPFTAGEE
                     SLREALSEYETVVCYKGGNRLPAVLRVAEEGGRLEGAVYGARLGIEDEEVLPAEALAG
                     REGTYLSTVVFTGRGAEFG"
     gene            1150..1923
                     /gene="cobM"
                     /locus_tag="VFJ72_10865"
     CDS             1150..1923
                     /gene="cobM"
                     /locus_tag="VFJ72_10865"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HET7480002.1"
                     /translation="MWFIGAGPGAPDLLTIRGARLIGEADVVIWARSLVHEGVLEHAR
                     NDAEIIESTDIPLESVRALYERAVLEDLKVARVHSGDPSLYGAVMEQIERCDEIGLEW
                     EMVPGVSSLGGAAAAIGRELTIPEVAQSVIVTRRPNRTPMPGNEDIRSFAAHGTTMAI
                     FLSAAKPRALQQDLLEGGYPPETPCAIVYRATWEDEQIIRCRLEELADRVRLAGFTRQ
                     ALILVGPGLGAGGTRSHLYDPTFSHMFRKAEPGADAAAS"
     gene            1907..3091
                     /locus_tag="VFJ72_10870"
     CDS             1907..3091
                     /locus_tag="VFJ72_10870"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HET7480003.1"
                     /translation="MPPPRKLREPSMPPSMAKVKGDKLRTGWTTGACSAAAAKAAARA
                     LVTGEPQEKVDIKLPKKAKPGGERRVEFAVERCDLDEDGTWAEAVIVKDAGDDPDVTD
                     RAHLTVRVSWSEEPGLHLDRGEGVGLVTKPGLGLAVGGPAINDTPRRMISYSVREVLD
                     PDERGVRVVISVPGGEKMAEKTTNARLGIVGGISILGTTGIVRPFSTAAWAASVVQAV
                     NVMGAQGHKTFVLSTGGLTEKAAMRLLPDLEDVNFIEVGDFTGQAIKQAVKNGLERCF
                     FVGMAGKISKLAAGVMMTHWTRSKVDNEVLAGITERAGGSDDLIEEVKSANTARHAYE
                     LWRAADLDRAPHLLCQQAADNLAEYAEGRLEVHVILVDFDTLEPTGASSGALELTTWS
                     SG"
     gene            3088..4290
                     /gene="cbiE"
                     /locus_tag="VFJ72_10875"
     CDS             3088..4290
                     /gene="cbiE"
                     /locus_tag="VFJ72_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HET7480004.1"
                     /translation="MNPITVIGLDGRPLDEEAEAAIRDAALVVGGERHLEMLAIEPDR
                     AVVLKGDLSAALARVEASNGPVAVLASGDPGYFGIVRLLGERFGREDLRVIPALSSVA
                     LAFARAGLSWDDAVTVSAHGREPRRAVNVCRARPKVAVLTSPEFGPAELAGMLAGLDR
                     AFIVAERLGEPDERVFRGDAATIAEMEWRDPNVVLVLDEERMAGRKGWISSAVESRQR
                     WALPEDQFEHQSGMITKSETRALVLARLGPGPGDLIWDVGAGSASVAIECARLGAAAI
                     AVERDPESCARIRRNVERHGVNVQIVEGEAPEALRDLPEPDAVFVGGTGGGFEEIVKL
                     AAVRARRSVVLALVGLERVVPAGKILEDCGLEVETTFLQTSRIKGVGSWHRIAPESAV
                     FVVCGSRT"
     gene            4233..5972
                     /gene="cobJ"
                     /locus_tag="VFJ72_10880"
     CDS             4233..5972
                     /gene="cobJ"
                     /locus_tag="VFJ72_10880"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HET7480005.1"
                     /translation="MAPHRSRERRLRSLREPNVIGLVAATANGRRNAAHLAEAWADAR
                     VYEGRPKEALRKAWDECDGIVLLLATGAAVRLIAPLLEDKRTDPGVVTVDDAARFVVA
                     LCGGHGGGSNALAERVAEALGATPVVTTASDALGVPALDSLGEQLGFRLEDHSDLAAV
                     GAALVSGERVNLVSDRRWPLGPLPENLVSSREPEPPLVLISDRIVDAPKPAVIYRPPS
                     LVVGVGCSRGATANEILELLDSSLREAGLSKKSVAAFASVDVKRDEAGLLEAAERLGV
                     PVRFYSADALSAADAPNPSSVVRDAVGTPSVAEASVLVSGAELVLEKRKSGMATVAIG
                     RLPVRGRLALVSLGPGEDALIPPLARDALAASELIVGLDQYVDRVRHLLRPGTRVLTP
                     PLGNEIERAELALAEARSGGSAALVSSGDVGIYAMASPVLELAGEDVEVVVVPGITAA
                     QAAASLLGSPLGHDHCSISLSDLLTPWEVIQQRVRAAAEGDFVVSLYNPRSKGRDWQL
                     GKVREILLQHRPPDTPVGIVRDAYRPTQQVTLTDLASLRPESVDMLTVVVVGSSQTKV
                     VAGRMVTPRGYLS"
     gene            5969..6172
                     /locus_tag="VFJ72_10885"
     CDS             5969..6172
                     /locus_tag="VFJ72_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HET7480006.1"
                     /translation="MKTTFAVTDQCAGCGACIRTCPEKAIQPAPRDYGSPLVVLEDRC
                     TGCAECAEVCPVSACIEVLLEDA"
     gene            6169..6753
                     /locus_tag="VFJ72_10890"
     CDS             6169..6753
                     /locus_tag="VFJ72_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HET7480007.1"
                     /translation="MRRVHPIETESYRIMRELLDLSHLGPLSRAVAERVIHASADLEY
                     AESLVLDEAALEGGLEALRNGAPIVTDVGMVAAGITGRQTRCFVSDPRARELSEDLGI
                     TRSAAGFRIAAEEVGAGAVWVVGNAPTALFELLETDTRPELVIGLPVGFVGAAESKEK
                     LIESGLPAVANHGRKGGSAVAAAALNALIYYEED"
     gene            6755..7699
                     /locus_tag="VFJ72_10895"
     CDS             6755..7699
                     /locus_tag="VFJ72_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HET7480008.1"
                     /translation="MRNTVGIRAKNGSSESGARPALLVVGHGSRDPRGAREFHELLDL
                     VRERNSSLAVEGGFIELSRPPISECVDRLAEGGARRVAAVPLMLLAAGHAKDDIPATL
                     VREKMNHPEMSFQYGRALGIRPELLELMDERVSGVVAEGEKAETAVLIVGRGSSDPDA
                     NSDVSKLSRLFYEGRPYPIVESAYVSMTPPNVHDGLERCRRLGAKRIVVFSYFLFTGV
                     LEERIREQSESFAAGNPDVEVRYAGYFGPHEKVADLLIERYEEAVGGDIRMNCDVCVH
                     RVALPGFEEKVGAPATPHYHPDEPGHHHGHVHDHHHHG"
     gene            7720..8763
                     /gene="cobT"
                     /locus_tag="VFJ72_10900"
     CDS             7720..8763
                     /gene="cobT"
                     /locus_tag="VFJ72_10900"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET7480009.1"
                     /translation="MLEIAADVQPLDGEAAERARERHLTLTKPPGSLGRMEDLGARLA
                     GMSGECPPPAPENPAVVVCAGDHGVLERGVSPWPKEVTAAMVKNFCEGGAAVNAIAKT
                     VGARVSVLDVGVALDLEQHPLLRGTKVRAGTDDLSRKLAMSREEAARAVMAGAGVAEE
                     LIESGGVDLLVTGDMGIGNTTPAACLIAAFTGQPAGSVTGRGTGIDDETLALKVAVVR
                     EALELHEPDADDPLGVLAAVGGLEHAALAGVILTGAAYGIPVVLDGVISDSSALVARS
                     LAPASVDYMIAGHLSAEPGARIALEHLGLEALLDLDMRLGEGTGGLLAVPLVQAAART
                     LGEMSTLEELGFG"
     gene            8760..9587
                     /locus_tag="VFJ72_10905"
     CDS             8760..9587
                     /locus_tag="VFJ72_10905"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET7480010.1"
                     /translation="MNGLSRQAIESLDSPLARVVEVHEALPSTQERARELAHAGTPHG
                     TLVIAEVQTGGRGRLGRRWGSPRGGLWMSLVLRPEFAAGLAARVTQMAAVGVTKALWE
                     IGVQARIKWPNDLLVDGRKICGILAESSVENVPVAAKRVVATVESGRRLDFVILGVGL
                     NANLDPVDLEVPDREVTTVRSELGRDVDMLDLLRALLSNLDAELGRIEDFGAVLEDWR
                     ALNCTLGERVRVRRFGETLEGRAADLSPEGALLLETRRGTVELFEGEIEHLRQGNAI"
BASE COUNT         1519 a         3039 c         3551 g         1480 t
ORIGIN      
        1 gaagccctct ccgcgaacga gcccatgcgc gagatcgtcc ggcggttcgc ctccgagggc
       61 cgtcccatcg tggccgagtg tggcggcctg ctatacctcg cccgcgagct ggatggccac
      121 ccgatgtgcg gcgtcctcga cacggcggcg cgcatgaccg aacggctcac gctcgggtac
      181 cgggaggcac gggctcccgt agactcggtg ctggccgcgg aaggtgtaac cgtgcggggc
      241 catgagtttc actactcggt agtagagccg ggtttcggtg agagcccggc ctgggagttg
      301 accggacgtg gagcggaggg attcgtcgcg ggcgggatgc acgccagcta cctgcacacc
      361 cactgggcgg cgacgccgga gctaccgcgc cggttcgtga gggcggcgac acggagcgcg
      421 gtggaggtcc gggcgtgagc gggcgactgg tgggtattgg agtcgggccg ggagacccgg
      481 aactgttgac gatcaagggc ctgcgcgcgt tgcgggaggc agatgaggtc ttcgtgccgg
      541 tggcggacac gggcgaggtc ggacgggccg aggcgacggt gcgcgagtac ctggagccgg
      601 ggcgcgtccg gcgtctgctc ttcgccctct ccgacgacgc ggaggcgcgg gagcgcaact
      661 ggacgaacgc cgcgcgtgag gtggcggaag ccctgcgggg ggggaaggtc tgcgcgttcg
      721 cgaccatcgg cgacccgaac gtatactcga cgttcacgta cctggcgcgg acggtgcgcg
      781 agtctatgcc ggaggtggag gttcagacgg tgccgggcat cacggcgatg caggacctgg
      841 cctcgcgcag cgggacggtg ctgctcgaag gcgacgagcg gctggcgctg ttgccgttca
      901 cggcggggga ggagtctctg agggaagcgc tctcggagta cgagacggtg gtctgctaca
      961 agggcgggaa tcgtctgccg gccgtgctgc gggtggcgga ggagggcgga cggctggagg
     1021 gcgcggtgta tggggcgcgg ctcgggatcg aggacgaaga agtgcttccg gcggaggcgt
     1081 tggccgggcg cgaggggact tatctatcca cggtggtctt caccgggagg ggagcggagt
     1141 ttgggtaagg tgtggtttat cggggcggga ccgggagcgc cggacctcct gaccatcagg
     1201 ggggcgaggc tcatcggaga ggcggacgtg gtgatctggg ctcgcagtct cgttcacgaa
     1261 ggcgtcctcg aacacgctag aaacgacgcg gagataatcg agtccacgga catccccctc
     1321 gaaagcgtgc gcgcgttata cgaacgcgcc gttctcgaag atttgaaggt ggcgcgggtg
     1381 cattccggcg acccgagcct gtacggggcg gtgatggagc agatcgagcg ctgcgacgag
     1441 atcgggctgg aatgggagat ggtccccggc gtctcatctc tgggaggcgc ggcggcggcc
     1501 atcggccgcg agctgaccat cccggaagtc gcccagtccg tcatcgtgac gcgccgcccg
     1561 aaccgcaccc cgatgccggg caacgaggac ataaggagct tcgccgcgca cgggacgacg
     1621 atggcgatct ttctctccgc cgcgaaaccc cgcgccctgc aacaggacct tctggagggc
     1681 ggttacccgc cggaaacccc gtgcgccatc gtgtaccggg ccacctggga agacgagcag
     1741 atcatccggt gccggctcga agagttggcc gaccgcgtaa ggctggccgg tttcacccgg
     1801 caggcgctca tcctcgtggg accgggactc ggtgcgggag ggacgagatc tcacctgtac
     1861 gaccccacgt tctcgcacat gttccgaaag gcggagccgg gagccgatgc cgccgcctcg
     1921 taagctccgc gagccgtcca tgccgccgag catggcgaag gtcaagggcg acaagctcag
     1981 gaccggctgg accaccggcg cctgctccgc cgccgccgcg aaggccgctg cccgcgcgct
     2041 cgtaaccggc gagccgcagg agaaggtgga tataaagctc cccaagaagg cgaagccggg
     2101 cggcgagcgc cgcgtcgagt tcgcggtcga gcggtgtgat ctggacgagg atgggacctg
     2161 ggccgaggca gtaatcgtca aggacgccgg cgacgacccg gacgtgacgg acagggcgca
     2221 cctcaccgtc cgcgtctcgt ggagcgagga gccgggcctg cacctcgacc ggggcgaggg
     2281 cgtgggcctc gtgacgaagc cggggctcgg gcttgctgtc ggcggaccgg cgataaacga
     2341 cacgccgcgg cgtatgatct cctactccgt ccgcgaggtg ctcgacccgg acgagcgcgg
     2401 cgtgcgcgtc gtcatcagcg tacccggcgg agagaagatg gcagagaaga ccaccaacgc
     2461 ccgcctcgga atcgtcggcg gcatctccat cctcgggacg accggcatcg tgcgcccgtt
     2521 ctcgacggcc gcctgggccg cgagcgtcgt gcaggccgtc aacgtcatgg gcgcgcaggg
     2581 ccacaagacc ttcgtgctct cgaccggcgg attgaccgag aaggccgcga tgcgcctgct
     2641 gccggacctc gaagatgtga actttataga ggtcggggac tttaccgggc aggccataaa
     2701 gcaggccgtc aagaacgggt tggagcgatg cttcttcgtc gggatggccg ggaaaatctc
     2761 caagctcgcc gccggcgtca tgatgaccca ctggactcgc tcgaaggtag acaatgaagt
     2821 attggccggc atcacggaga gagccggtgg ctccgacgat ctgatcgagg aggtcaagag
     2881 cgccaacacc gcccgccacg cctacgagct ctggcgcgcc gcggacctgg accgcgcgcc
     2941 gcacctcctc tgtcagcagg ccgccgacaa cctcgccgag tacgcggagg gccggctgga
     3001 agtccacgtc atcctggtgg acttcgacac tctggagccg accggcgcca gttccggcgc
     3061 cctcgaactg acaacctgga gcagcgggtg aaccccataa ccgtcatcgg gctcgacggc
     3121 cgcccacttg atgaagaggc tgaagcggct atccgggacg ccgcactcgt agtcggcggc
     3181 gaacgtcacc tggagatgct cgccatcgag ccggaccgcg cggttgttct gaagggcgac
     3241 ctttcggcgg cgctcgcgcg ggtcgaagca tccaacggtc cggttgcggt gctcgcgtcg
     3301 ggcgacccag gctacttcgg tatcgtgcgc ctgctgggcg aacggtttgg cagagaggac
     3361 ctgcgcgtga tccccgctct ctcctccgtc gctctggcct tcgcccgcgc cggactctcc
     3421 tgggacgatg ccgttaccgt cagcgcccac ggccgcgagc cgcgccgcgc cgtcaacgtc
     3481 tgccgcgcgc gcccgaaggt cgccgtcctc acctccccgg agttcggtcc cgctgaactt
     3541 gccgggatgc tggcgggtct ggaccgggcc ttcatcgtcg cggagaggct cggcgagccg
     3601 gacgagcggg tgtttcgtgg ggacgcggcg acgatagcgg agatggagtg gcgcgacccg
     3661 aacgtggtgc tggtgctcga cgaggagcgt atggcgggac ggaagggttg gatctcatcg
     3721 gccgtcgaga gccgccagcg atgggcgctc ccggaagacc agttcgagca ccaatccggc
     3781 atgatcacca agagcgagac tcgcgcgctc gttctggccc gcctcggccc cggtcctgga
     3841 gatctgatct gggacgtcgg cgcgggcagc gcctcggtag ccatcgagtg cgcccgcctc
     3901 ggagccgccg caatcgccgt cgaacgcgac cccgaaagct gcgcccgcat ccgccgcaac
     3961 gtcgaacgcc acggcgtaaa cgtacagatc gtagaaggcg aagctccaga agcattgcgc
     4021 gacctgccgg agcccgacgc cgtcttcgtc ggagggacgg gcggcgggtt cgaggagata
     4081 gtcaagctcg ccgccgtccg ggcgcgccgg tccgtcgttc tggccctcgt cggcctggag
     4141 cgggtcgtgc cggcgggcaa gatcctcgaa gactgcggcc tggaggtcga gactacattc
     4201 ctgcaaacat cccggataaa gggtgtcgga tcttggcacc gcatcgctcc cgagagcgcc
     4261 gtcttcgtag tctgcgggag ccgaacgtga tcggtctcgt ggcggcgaca gcgaacggcc
     4321 gccgcaacgc cgcccacctg gctgaagcct gggccgacgc ccgcgtctac gagggtcggc
     4381 ccaaagaagc gctgcgtaaa gcatgggacg agtgcgacgg catagtcctc ttactcgcca
     4441 ccggcgccgc cgtccgcctc atcgccccgc tgctcgaaga caagcgcacg gaccccggcg
     4501 tggtcaccgt ggacgacgcg gctcgcttcg tcgtagccct ctgcggcggc cacggtggcg
     4561 gctctaacgc gctggcggaa cgggtcgcgg aggctctcgg agcgacgccc gtggttacta
     4621 ccgcgagcga tgctctgggc gttccggctc tagactctct gggcgagcag ctcggcttcc
     4681 gtctcgaaga ccattcggac ctcgcggccg tcggcgcggc gctcgtttcg ggggagaggg
     4741 tcaacctggt atccgaccgg cgctggcccc tcggaccgct gccggagaac ctggtctctt
     4801 cacgggaacc ggaaccgccg ctggtcctga tatccgaccg gatcgtggat gcaccgaaac
     4861 ccgctgtcat ctatcgtccg ccctcgctcg tcgtgggcgt cggatgtagc cggggagcga
     4921 cggcgaacga gatactggaa ctgctggact cctctctccg cgaagccggt ctctcaaaga
     4981 agagcgtcgc cgctttcgcc agcgtcgacg tgaaacgcga cgaggccgga ctgctggaag
     5041 cggcggagag gctcggtgtg ccggtgcggt tctattcggc tgatgcgctc tccgcggcgg
     5101 acgctccgaa tccatcgtct gtggtgcgcg atgcggtagg gacgccgagt gtggcggagg
     5161 cgtcggtgct tgtctcgggt gcggagttgg tgctggagaa gcgcaagtcg gggatggcga
     5221 cggtggctat cgggcggctg ccggtgcggg ggaggctggc gctggtgagc ctggggccgg
     5281 gggaggacgc cctgatccca cctctcgccc gcgacgctct tgccgcctcg gagctgatag
     5341 tcggcctcga ccagtatgtg gatcgcgtcc ggcacctgct gcggcccggc acgcgggtgc
     5401 tgacgccgcc gctcggcaac gagatcgaac gcgccgaact ggcgctcgcc gaggcccgct
     5461 ccggcggcag cgccgcgctg gtttcgagcg gtgacgtggg catctacgcg atggcatctc
     5521 cggtgctgga gctggcgggc gaagatgtgg aggttgtagt cgtgccgggc atcacggcgg
     5581 cgcaggcggc ggcctcgctc ctgggctctc cgctggggca cgatcactgc tccataagcc
     5641 tctccgacct cctaactccc tgggaggtga tccagcagcg ggtgcgggcg gcggcggagg
     5701 gagacttcgt ggtctcgctc tacaacccgc gctcgaaagg ccgcgactgg cagctcggga
     5761 aagtacggga gatcctgctc cagcaccgcc cgcccgatac tccggtcggc atcgtccggg
     5821 acgcctaccg cccgacgcag caagttacgc tgacggatct cgcctcgctg cgccccgaga
     5881 gcgtggacat gctgacggtc gtggtggtcg gtagctctca gactaaagtc gtcgccgggc
     5941 gtatggtgac gccgagggga tacctctctt gaagaccacg ttcgccgtca cggaccagtg
     6001 tgctggatgt ggcgcttgca taaggacatg cccggagaag gctatacagc cggctccgag
     6061 agactacggc tcgccgctcg tcgtcctcga agaccgttgc acgggctgcg ccgagtgcgc
     6121 ggaggtctgc ccggtctccg cctgcatcga agtgcttctg gaggatgcgt gagacgagtc
     6181 catcctatag agaccgagag ctaccgcata atgcgcgaac tgttagacct ctcgcatctc
     6241 gggccgctct cccgcgccgt ggccgagcgc gtgatccacg cctccgccga cctggagtac
     6301 gcggagagtc tcgtattgga cgaggcagct ctggaaggcg ggctggaagc cttgcggaac
     6361 ggcgccccca tcgtcacgga cgtcgggatg gtggcggccg ggatcacggg ccgccagaca
     6421 cggtgcttcg tctcggaccc tcgcgcccgc gagctctcgg aagacctcgg catcacgcgc
     6481 tcggcggcgg gcttccggat cgcggcggag gaggtcggag cgggggccgt ctgggtcgtc
     6541 ggtaacgcgc cgacggcgct cttcgagtta ctggaaacgg acactcgtcc ggaacttgtg
     6601 atcgggcttc cggtcgggtt cgtcggggcg gccgagtcga aggagaagtt gatcgagagc
     6661 ggcctgcccg ctgtggcgaa ccacgggcgg aaaggcggct cggcggtcgc ggcggcggcg
     6721 ttgaacgcgc tcatctacta cgaggaggac tgatatgcgg aacacggtcg gcatcagagc
     6781 caaaaacggg tcgagcgaga gcggggccag accggcgctc ctggtcgtgg gccacggcag
     6841 ccgcgacccg cgcggcgcgc gcgagttcca cgagctgctc gacctcgtgc gcgagcgtaa
     6901 ctcttctctt gcggtggagg gcgggtttat cgagctatcc cggccgccca tctcggagtg
     6961 tgtggaccgg ctggcggagg gtggggcgcg ccgcgtcgcc gccgtgccgc tgatgctcct
     7021 cgccgccggt cacgcgaagg acgacatccc agctactctg gtacgcgaga agatgaacca
     7081 cccggagatg agctttcagt acggccgcgc cctgggtatc cggcccgagc ttctggagct
     7141 gatggacgag cgcgtctccg gcgtcgtggc ggagggagag aaggctgaga cggccgtgct
     7201 catcgtcggg cgcggctcct cggacccgga cgcgaactcc gacgtctcga agctgtcgcg
     7261 cctgttctac gagggccgcc cgtatccgat agtcgagagc gcctacgtca gcatgacgcc
     7321 gccgaacgtc catgacggtc tcgaacgctg ccgcaggctc ggcgcgaaac gtatcgtggt
     7381 cttctcctac ttcctcttca cgggcgtcct ggaggagcgc atccgggagc agagcgagtc
     7441 gttcgccgcc gggaacccag atgtcgaggt gcgttacgcg gggtacttcg ggccgcacga
     7501 gaaggtggcg gacctgctga tcgagcggta cgaggaggcc gtggggggcg atatccgcat
     7561 gaactgcgac gtgtgcgtcc accgggtcgc gttgccggga ttcgaggaga aggtcggggc
     7621 tcccgcgacg ccgcactacc acccggacga gccggggcat catcacggcc acgttcacga
     7681 ccatcaccat cacggttagt catggcgttc gaggataggg tgcttgagat tgctgctgat
     7741 gttcagcctc tcgacgggga ggctgccgaa cgagcgcggg agcgccatct cacgctcacg
     7801 aagccgccgg ggagcctcgg gcggatggag gacctcggtg cgcggctcgc ggggatgtcg
     7861 ggtgagtgcc cgccgccggc gccggagaac cccgcggtgg tggtgtgcgc cggggaccac
     7921 ggggtgctgg agcgcggcgt ctcgccgtgg ccgaaagagg ttacggcggc gatggtgaag
     7981 aacttctgcg agggcggcgc ggcggtcaac gccatcgcga agaccgtcgg ggcgcgggtg
     8041 agcgtgctgg acgtgggggt ggccctagac ctggagcagc atccactctt gagggggacg
     8101 aaggtccggg ccggcacgga cgacctgagc cgcaaactgg ccatgagccg cgaggaggcg
     8161 gcgcgggcgg tcatggccgg agccggggtc gcggaggagt tgatcgagag cggcggcgtg
     8221 gacctgctcg tcacgggcga catgggcatc gggaacacca cgcccgccgc ctgcctcatc
     8281 gccgcgttca ccggacagcc cgccgggagc gttaccgggc gcggcaccgg catagacgac
     8341 gagacgctgg cgctaaaagt agcagtagtt cgggaggcgc tggagttgca cgagccggac
     8401 gccgacgacc ctctcggcgt cctggcggcg gtcggtggac tggagcatgc ggcgctggcc
     8461 ggtgtgatcc tgacgggcgc cgcatacgga atcccggtcg tcctggacgg tgtgatctcg
     8521 gactcctccg ccctcgttgc ccgctccctc gcccccgcca gcgtcgatta catgatagcc
     8581 ggacacctct ccgcggaacc cggagcgcgg atagctctgg agcatctggg actggaggcg
     8641 ctgctggatc tcgacatgcg cctgggagag gggaccggcg gactgctagc cgtgccgctg
     8701 gtgcaggcgg cggcgcggac gctgggcgag atgtctaccc ttgaggagct gggattcggg
     8761 tgaacggtct ctcgcggcag gccatcgagt ccctcgacag tccgctcgcc cgtgtggtag
     8821 aggtccacga ggcgctgccc tcaacccagg agcgcgctcg cgagctggcc catgccggaa
     8881 cgcctcatgg cacgctcgtc attgcggagg tgcagaccgg cggccgtgga cggctcggta
     8941 ggaggtgggg gtcgccgagg ggcgggctct ggatgtcgct ggtgctgcgg ccggagttcg
     9001 cggcggggct cgcggcgcgc gttacgcaga tggctgccgt aggggtgacg aaggcgctct
     9061 gggagatcgg ggtccaggcc cgcatcaagt ggcccaacga cctgctcgtt gacggaagga
     9121 agatctgcgg catcctggcc gagtccagcg tcgagaacgt ccccgtcgcc gccaagcgcg
     9181 tcgtcgctac ggtggagagc ggacgccgcc tggacttcgt gatcctcggc gtcggcctga
     9241 acgcgaacct cgacccggtg gacctcgaag tcccggaccg ggaagtgacc acggtgcgct
     9301 ccgaactggg ccgcgacgtg gacatgctgg atctcctgcg cgccctgctc tcgaacctgg
     9361 acgcggagct tggtcggata gaggacttcg gcgccgtcct cgaagactgg cgcgccctca
     9421 actgcaccct gggggagcgc gtgcgcgtcc ggcgcttcgg cgagaccctc gaagggcggg
     9481 ccgcggacct gagcccggaa ggcgcgctgc tgctggagac gcggcgcggg acagtggagc
     9541 tgttcgaggg agagatcgaa cacctccgcc agggcaacgc catatgaag
//
LOCUS       DASSMB010000184        22029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1567474, whole genome shotgun sequence.
ACCESSION   DASSMB010000184 DASSMB010000000
VERSION     DASSMB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 22029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22029
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567474"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VFJ72_10910"
     CDS             complement(<1..634)
                     /locus_tag="VFJ72_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480011.1"
                     /translation="MKNAPPQYERMAAAVRAARSRSHSEEQALRHVEAAATVDPVGAA
                     ERLVGFADLEQRQCTQDVRRHFGIEVRATVGDTRAFVVDISGDRIRFAGRGGYKPIAA
                     LSIVRPECLPQTFIEETYLDHLLVSYEIADLAHRAMLEIGWDVKEAGHGVEDMSWAMC
                     QVFVVLYRHRFVFSGTFMTDSGLHPELRKWHTVQMAGWVGSKISLEKNKRL"
     gene            complement(719..1360)
                     /gene="nth"
                     /locus_tag="VFJ72_10915"
     CDS             complement(719..1360)
                     /gene="nth"
                     /locus_tag="VFJ72_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7480012.1"
                     /translation="MPAAPTSEVIPRLKQEYPGATTELNWSNPLELLVATMLSAQTTD
                     VRVNAVTKDLFAKYPTAEHYAVADLSDLEEDIRPTGFYRNKAKSLQGMARALLEDHDG
                     EVPRTMRELVALPGVGRKTANVVLGNAYGVDEGIVVDTHVRRLSNRLGLTTEQNPEKI
                     EQDLMRVVPTGDWTVFSHLLILHGRRVCKARKPDCPGCVLSDICPSAFEYKTG"
     gene            1478..2101
                     /locus_tag="VFJ72_10920"
     CDS             1478..2101
                     /locus_tag="VFJ72_10920"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HET7480013.1"
                     /translation="MKWLKVYGLPKLPLFLARWAMKLGLPGPTWYKWKAASAGTGVIL
                     DEMIRAADDLGYDGQKVGKLAMSRIGEKQGRDLREQLGVKTMKDTVDVVMLANRFFDI
                     EITLTETKDGEYVINADRCPWFGGMGQEGVIGWDVKPCAAFSTYEKAMVNAINPNVKL
                     SYTEKRTTGGHTCRGVYQYRDAELGDGPPEGVQPEMVSIGKKPSKDG"
     gene            2107..2706
                     /locus_tag="VFJ72_10925"
     CDS             2107..2706
                     /locus_tag="VFJ72_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET7480014.1"
                     /translation="MERIEIDTARGKERVPPGQYLVGDRWPILTYGSTPAFKPENWDF
                     VMEGLVENPIRFTWEEWNDLPTVEVKADMHCVTSWSKLDNVWTGVQAKHLLEMVRPKP
                     EARFFSAFCDGGYTTNLPVEELYEEDVLFATHHNGEPLEAGHGAPMRLVIPRLYAWKS
                     AKWLRGLEFLDEDKPGFWEQNGYHNYGNPWREQRYSFSF"
     gene            complement(2720..3670)
                     /locus_tag="VFJ72_10930"
     CDS             complement(2720..3670)
                     /locus_tag="VFJ72_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7480015.1"
                     /translation="MNSGGTKVLYVAGLGRSGSTILANSLGQIEGFFSAGELNFIWKH
                     NVLENRLCGCGEPFHECPVWTRVMDEAFGGMDGVDAREMMRLQSLGARTRHIPAMLTR
                     QGEISIRERIGPLLDNTAKLYRAIGVVARSRVIVDSSKEPAYGYAVGMAPGLDLYVLH
                     LVRDSRAAAYSWSKKKPQPDTEDREFMHRHSPAKSAALWDSWNAAAETLWRRTPERYL
                     RLRYEDFVVNPRQSFERILKLIGEEGSELPLASEREVKLGISHTVSGNPNRFDTGSVE
                     LRPDEAWKKMMKPRDRALVTALTLPLLKRYHYAVAPGSNR"
     gene            complement(3663..5414)
                     /locus_tag="VFJ72_10935"
     CDS             complement(3663..5414)
                     /locus_tag="VFJ72_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET7480016.1"
                     /translation="MEENPRRDDAATSKSPDFEHQEPGETLTPDETAADRERPPDAAG
                     DAPSGVPGVSAADTLGRDKDDTYVARVARGAGISTAGQGIGRVLGYLAQIIIAQTFGL
                     QAYGFYSLGVAASNGAQILARFGMENGVVRYVAHYRAQEDTARVRGTIIQSVMVALLL
                     SLALSAVMFFGAGFAADLYYKKSAMEPVLKAFAVSLPFFTFMMMVLWATQGFQTVTYA
                     SYVQQIIRPALFLLLVPLFYLLGAGVIGAVAAYGVSMALGCVVSVYFLRKLFPPLFDR
                     RVRPKFETRALFSVSVPMSIATGAQYANTWSAIWVLGYFAAGTPVGVFQAAARTATFS
                     TIVRFAFSGIFSPIISSLHAQGDRENLGLLYKDISRWIFTGAFGIFLVIILLARDALV
                     LGFGEQAAGGILALIIVAAAQLYSSSVGPTPRMLAMTGNQNIAMFATAAAALTGLAVS
                     VTLISLAFTPESKILGAAVGMASGIVAENTATLLAVRRRLGFWPYNLAWLKPLAAGLI
                     SAAVAYVVGMLVPMPAIPSILLVGTVFGLGYLALLALFGLTDTDREFLGAFWNVAKRV
                     LRRVRRLGSRNRESGGE"
     gene            complement(5475..6071)
                     /locus_tag="VFJ72_10940"
     CDS             complement(5475..6071)
                     /locus_tag="VFJ72_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480017.1"
                     /translation="MPGWLKTLLRWTALFCVPGGVLWSLSPLGVHLSEWKYKTPNVFW
                     KLFPSVPLLLMVGLVGLYFWTRGRHRLSWEIGFWVTLLGLILVFIGDTAEFWLGVDDT
                     YLLLAPAYRTFRLGLLVLAVGSILFGASNLRSGTLPRWISLPFAVGSVGVLVAVSKDI
                     GTVGAALWVAYGICWVWIGLGLLLEALISFRRKKRAGY"
     gene            complement(6111..7445)
                     /locus_tag="VFJ72_10945"
     CDS             complement(6111..7445)
                     /locus_tag="VFJ72_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7480018.1"
                     /translation="MNVILIVLDSLRKDHIGAYGSDRVKTPTLDALSKESLRFTQAYP
                     ESVPTIPARRAIHTGIRTWPFRNWVPQKGETFFPAGWQRIPEDQTTLSEILLPQGYNT
                     ALITDTQHEFKASMNFQRGFDIFDFIRGQERDHYHSVLRVSDDMVDRNTVSGNTSGMR
                     GKVRQYLANTMNRKTEEDWFAPRVFLRAGEYLEVAREGQPFFLLVDSFDPHEPWDPPE
                     KYVKLYSDDYDGPDPIVPNYSDAGWITDRELERMKALYSGEITMVDHWLGNFLNKVSD
                     LGLLDNTLILVLSDHGVALGEHNDTGKPDWALYPELTDIPFFIRHPEGKGAGETSDYY
                     ASIHDVAPTVLGATSIEQKDPMDGEDLSVILDGKDPAPRSHFTLGYNDYVRSHDDRYV
                     MISRNDGAEAKLFDIQSDPDMKQDIAGDNRDIVQRMFNDYALRDAGGSIPRY"
     gene            complement(7737..8702)
                     /locus_tag="VFJ72_10950"
     CDS             complement(7737..8702)
                     /locus_tag="VFJ72_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HET7480019.1"
                     /translation="MKPRALVTGGAGFLGSHLCERLLGEGYRVICMDNLRTGSLENIA
                     HLQDSPDFEYIHHDVTLHIEVEGELDEVYHFASPASPKDFERIPIPILKAGALGTYNA
                     LGLALAKGSRLMLASSSEVYGDPLVHPQPEEYWGNVNPIGVRGVYDEAKRYAESITMA
                     YHRHHGVDTRIVRIFNTYGPRMRPDDGRMIPNFVSQALASEPITIYGDGCQTRSVQYV
                     DDLIEGTIRLMRSREVRPVNIGNPVEYPVSEVAGMVLDLSGSRSKITFEPLPQDDPRQ
                     RCPDITRAREALGWEPLVPAREGLRATLAWFTSRPRRSGVSPARG"
     gene            complement(8727..10136)
                     /locus_tag="VFJ72_10955"
     CDS             complement(8727..10136)
                     /locus_tag="VFJ72_10955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET7480020.1"
                     /translation="MTESFNVGVVGTGYVGLVTGACLAYLGHRVTCVDRDEERIAELV
                     AGRVPIYEPGLDELVSGGTRRGRLSFAGAEGVSEVARSADVVFVAVNTPQGEDGSADL
                     SNVAAVARSIGKTLSETERERPLVVVNKSTVPVGSGDYVSMLIEEGAEDGAGDEAEGV
                     ATVRTKFLVVSNPEFLREGSAVYDSLFPDRIVLGADSREALDTMRALYEPIIQQSFPT
                     DVDPRPRAAVPFVTTDLASAEMIKYAANAFLATKISFINEISAICELVGADVSNVATG
                     IGLDERIGARFLNAGIGWGGSCFPKDVAALRSIAREYDYEPALLDATVTVNERQRRQV
                     ISKLQRDLHTLKGKRIALLGLAFKPNTDDLREAPSLEIARALHARGARAVGYDPVAGK
                     AAARLMPDLKVVFDPYEALAGTHAAVVVTEWEEVRSLDLDRAADLMEEPRLLIDGRNV
                     FDPAAVRECGLLYRGFGRE"
     gene            10426..10809
                     /locus_tag="VFJ72_10960"
     CDS             10426..10809
                     /locus_tag="VFJ72_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480021.1"
                     /translation="MRKLTILAVMLTMALMAAAPASADTVTADGLIAGTLEGGVVVAG
                     NPNLANGAPTLSLGDDVAAAGSQVPPDAFAGGFNIPFTVNVADAGVGTTIEDDVIAGS
                     AFLDTNFVEAGSGSLSVDTQVGFLD"
     gene            complement(10870..12237)
                     /locus_tag="VFJ72_10965"
     CDS             complement(10870..12237)
                     /locus_tag="VFJ72_10965"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET7480022.1"
                     /translation="MKTLLYIKTLRRILSVSALLLADAAALVLGLAGSALLIEGETSL
                     DQALYLAPALLAVWITLLAAFHLYDRARVRRNPGALLAATLSWTGIALAGVAIYPESG
                     LRAAGVVVAASLSLLVSGALRLLYEQGIEMVYRRGFALAPMVIIGNATERERIRRMML
                     DAPGAYPVVGEIDLQDSVDLAALRQLLDGTQARGVILAGAERLADEDLLDLMRSVRLR
                     GIRMRAVPGALTLLRNQPILSQSMGLPLLEVRYPRLDNTQRAMKRALDITLSAAGLIF
                     LSPLFLAISLAIRLDSPGPAFFRQKRAGVDEKVFICYMFRSMYEDAESRQEEVEALNE
                     ADGPVFKMKRDPRVTPVGRFLRRWSIDELPQLINVLKGEMSLVGPRPLPLRDFERMGE
                     LHKRRLAAIPGMTGYWQISGRSNLSFEEMVRLDLYYIENWSLSFDLKIILRTLGAVLR
                     REGAY"
     gene            complement(12295..13476)
                     /locus_tag="VFJ72_10970"
     CDS             complement(12295..13476)
                     /locus_tag="VFJ72_10970"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39 family protein"
                     /protein_id="HET7480023.1"
                     /translation="MPVLTGAMMLAFACLMLLTPGEAGAVSSYIDFDRHSTYKALASG
                     TKSGVYVKKLSGGAGAVRLRPGVRSGTLTSRAYLPSARFDTLLPSWNATTPAGTYIQT
                     EVRVRSGGNWTPWFNMGVWARGTETIKRHSVNGQSTTNWQVLTDTLQSKGTIFADAYQ
                     YRLKLSSKKSGASPDVRQLAFTVSNSYRHGEFLGVAGSSALWGKDLPVPERSQMIYPN
                     GGEVWCSPTSLSMVMAYWSNRTGQGSLNQPVPTVAAGTYDYTYRGNGNWPFNTGYASA
                     YGLRASVNRFSSLGQVERWIASGVPVVASISWGSGQLAGAPIPASNGHLVVIRGFDKT
                     GKYVIVNDPAASSNSGVRRVYQRDQFADAWLTRGSGGVAYLVYPKGWKVPDQTYSRGS
                     W"
     gene            complement(13520..14299)
                     /locus_tag="VFJ72_10975"
     CDS             complement(13520..14299)
                     /locus_tag="VFJ72_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HET7480024.1"
                     /translation="MRPVDRVDVLGVGVDPLTAEELEAVVRRLAGSEARAVVLNVNAH
                     ALNLACRDAALRDCFNRADVVFCDGAGVRLAARLLGSRIPARITYADWMWRLAALASE
                     EGFSLYLLGARPGVVERAADRLAGSYPNLRIAGAHHGHFDHATGASENEMVVREINAA
                     APDILIVGFGMPLQERWLMENRYRLNARVALTGGAVFDYVAGELRRGPRLLTDNGFEW
                     LARLLIEPRRLWRRYLIGNPLFLARVLRQRISGKGRRGPAR"
     gene            complement(14287..15408)
                     /locus_tag="VFJ72_10980"
     CDS             complement(14287..15408)
                     /locus_tag="VFJ72_10980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7480025.1"
                     /translation="MDRPPVIILAGVRWDFLWQRHQTLATLFARAGYPTTFVETTGLA
                     TPKPAPATLRKLLARARRSSGSKIPSSEANLAVYSPLTLPPTLKIFRRLNTRFFVPRV
                     VEDLRKSTGPEPVIIAYPPTRTTLDLISGLRPRLVLYDCSDDYAAFPGVPKDIAATEH
                     ELLRRSDLVSCTSTHLLEKVRPERPDAFLSGPGVDYERFATVPSRRADIRTVCFFGHL
                     GEERIDFAVLRTIARAGFEVRIIGGMGRVERGFLKLPGIDYRGEVSHADLPSAFADVD
                     AFVLPYRMNALTKGISPAKTYECLATGRPVVAAPLPAMMELADYVYLAERPEDYVKAL
                     WEIAASENEERRRARQQLARENSWELRFADLEEKILCAL"
     gene            complement(15390..16334)
                     /locus_tag="VFJ72_10985"
     CDS             complement(15390..16334)
                     /locus_tag="VFJ72_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7480026.1"
                     /translation="MSGLRERLLGGLYLDLNRDYRNTVFLAGSGRSGTTWLADIINYR
                     REYRFIFEPFNPERVGICRDFYSKQYLRPDDRREEFLDPARKILSGGVRSAWTDRFNR
                     SFLARRRLVKDIRANLLLGWLHANFPEMPMVLLLRHPCAVAASRMALGWRDNLSETMR
                     QEDLVEDFLRPMEGGIRSAKTPFERHLFLWCVDNYVPLMQLSQNEAHLVFYENLVARP
                     EEEIPRLFDALGVDLDERVYEKMKRPSPLSRNTSMRQPPDDWRERIDRNQLRRALEIL
                     ALFGLDRVYGEDSMPDTGAAREMMRSRAGETSRWTARP"
     gene            complement(16355..17962)
                     /locus_tag="VFJ72_10990"
     CDS             complement(16355..17962)
                     /locus_tag="VFJ72_10990"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HET7480027.1"
                     /translation="MRAKRNPVCEDGSLSTRKTSPDDGYDDYVARIARGVGVSSAGQG
                     VGRLLGYATRVALARMHGPAQLGFYALGVTLVQVVNVLAQFGLDNGVVRYVAHYGAGG
                     DTARVRGTILQALGVTFALSIMLSGGVFFGAGFLAEEVFGKPFLETMFRAFSVSIPFF
                     TLMSMTLWATQGFQTVKYATLVQQVLRPLANLILVVIFYLLGVEILGAVAAYMLSMVL
                     GAVAALYYLKKVFPELLESGVRPRYESRELSAVSGPMIVANLTQYVNLWTAVIVLGVF
                     EPAPVVGVYDVAARTAGLSMLVLISFGGIFSPMASGLHQQGLTRDLGYLYEDVSRWAF
                     TGALLIFVVTAMLSRDVMLVFGEEFSQGWTVIVVIAAAQLFNSSVGPTARLLAMTGHQ
                     KAVMVSTVGSALLAVAASLLLVPPFGILGAAAATAAALVASNVATLYFVRRRLGFWPY
                     SRRYAKPLLAGVASAAALYLVRSILLEAGLPALLVCAPVALAVFAALLLSLGLSPSDR
                     QFLASFRASLGRHARQTSPRGPGEKTG"
     gene            complement(18023..19636)
                     /locus_tag="VFJ72_10995"
     CDS             complement(18023..19636)
                     /locus_tag="VFJ72_10995"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7480028.1"
                     /translation="MSEETGWTFSKEDREPGRDKVRRPGRMLMLFKALVALALLGVTA
                     YGMINDGLYSDDLWLPVAVGILALLLVTVFVRGYYVDVPAAGWALFALLVALAAIKGV
                     SITWTLSETETIKEFLRCSMYVAAFLLALGALNSGRQVAPLMDVAVLIVVAVAGYGLM
                     QKISPVQYPVTSLDKVRIDSTLDYVNTFAMVLGMGVVLALARMARLRNAVLRGLYAVF
                     VLAFLVALLLTVSRGGIASLGVGLMALFILTSDRLQIFANLALVSIPGAWLFWEMKDL
                     KGLWSLVPNEQKIADGTAFRDYLIIALAVAFVLQAGYSLVFSRYELLPLGRRTLGALA
                     LGGVVLVGLAAAFVVVSQYGGVEKTYQALVNNPVNTKNASQRLASASIGFRADYWKVA
                     WDEWKEHPLTGSGAGTFQYVWLKNRPITTGVKQVHNLYLEQGTETGIFAFLALVSFVA
                     LLLGYTARAAWRSGARGDRRVLLSGLVAAAVVYLVSSVIEWHWYIPPSTLFFFILAAI
                     AVKLAAEKDWETSEEIIAEGGRGPAVGKQ"
     gene            complement(19633..20247)
                     /locus_tag="VFJ72_11000"
     CDS             complement(19633..20247)
                     /locus_tag="VFJ72_11000"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HET7480029.1"
                     /translation="MEVLLDPASPVRRHYAKLAQNLLSLEDRVRSMVVTSPETGAGCS
                     SVCIGLGAALAEMGKRVVVVDCNLGRPNLHRMLGEPNFIGLTSCLQSGRALENYGHDL
                     VPGLLAVPTGPVSSDPVARIESQRFVEVVRGIEAHRDIVILDAPVAEKVIESPALSSQ
                     FDGVLLVIHASRTSKNTAREVTDDLLDAGVNLLGVVLNGAGESV"
     gene            complement(20279..21019)
                     /locus_tag="VFJ72_11005"
     CDS             complement(20279..21019)
                     /locus_tag="VFJ72_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HET7480030.1"
                     /translation="MRSINRIGRQGWQRARRNDLALPEILHVLWGWRRLISIIVLSLL
                     IVGLTFALLRERAYTAEAIVLVEPQQEVPAEDMEPFLRDVTGAVTSSEDFIVDVRRKA
                     GWKGTPEEFARRLDVQSLVTSDGTPTIRVRFESVDAEEAARTANAYAALFTERVDLLN
                     DRQLAGGALAADARVASKASLPDRPSGAGPLFYVAGAIVAGMLIGGGMALLLENRARS
                     WRDARDAELTLRAPVLGVIPEYPSAEEV"
     gene            complement(21006..21815)
                     /locus_tag="VFJ72_11010"
     CDS             complement(21006..21815)
                     /locus_tag="VFJ72_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7480031.1"
                     /translation="MSSIVFKISAGFLVGALVVIAGALFLSNHYTDEQRRLAAAGDTK
                     GAEEAAQKAIRLDPFDTDPLEAQSFLLQQQGLNDQAAEALQEAVKRDPNNYMPHLMLG
                     NLQMVKLNDLEGAARSYRQVLELNPRATVARNGLAQALIRQGKLEEAKKEYEKIREEK
                     EITDQGLYDLGRIYVRTGEPKEGYKAILQARRRSSKGLEQLKGSAKTEREDLIQSMDL
                     ALADALVVEGKYNRALKVISESSSDQAPALYELLSSDPEAYRDSVVNSEIY"
BASE COUNT         4266 a         7071 c         6904 g         3788 t
ORIGIN      
        1 aaaggcgctt gttcttttcg aggctgattt tcgatccgac ccaaccggcc atttgcacag
       61 tgtgccactt acgaagctcg gggtgtaggc ctgagtctgt cataaaagtc cccgagaaaa
      121 cgaacctgtg ccggtacagc acgacaaaga cctggcacat cgcccacgac atgtcctcga
      181 cgccatggcc agcttccttg acatcccacc cgatctcgag catagcacga tgggccaagt
      241 cggctatctc atacgaaacc agaagatggt ccagatatgt ttcctcaata aatgtctgtg
      301 gtaagcattc cggccgaaca atcgaaagag cggcaatggg cttgtagccg cctcgtccag
      361 cgaatcgtat cctgtctcct gaaatgtcga ctacgaaagc cctggtatcc ccgaccgtag
      421 cacgaacctc gatgccgaaa tgacggcgga cgtcttgcgt gcattgccgc tgctccagat
      481 cagcaaaccc caccagacgc tccgccgcgc ctacagggtc cacggtcgcg gctgcttcta
      541 cgtgtctcag cgcctgttcc tccgagtgcg accgacttcg cgcggctcgc acggcggcgg
      601 ccatcctctc gtactgcggc ggcgcgtttt tcaccgtccg gcccctctct cggtctaggc
      661 tctcgccaat tctaaccgct cggcgccgtc cccggagagc aaagcgctga acctcacctc
      721 agccggtttt gtactcgaat gccgatgggc agatgtcgct caaaacacag ccgggacagt
      781 cgggtttgcg cgccttgcag acacgccggc cgtggaggat aagcaggtgc gagaagaccg
      841 tccaatcccc ggtgggtacg acgcgcatga ggtcctgctc gatcttctcg gggttttgct
      901 cggtggtgag tccgagacga ttggagaggc gccggacgtg ggtgtccacc acgatcccct
      961 cgtccacccc gtaggcgttg ccgagcacga cgttggcggt cttgcgcccg acgccgggga
     1021 gggcgaccaa ctctctcatc gttcggggca cttcgccgtc gtggtcttcc aggagagcgc
     1081 gggccatacc ctgcaaggac ttcgctttgt tgcggtagaa gccggtcggc cgtatatcct
     1141 cctccaggtc ggagagatcc gctacagcgt aatgttcagc agttgggtac ttcgcaaaga
     1201 ggtcctttgt tacggcgttt acacgaacgt ccgtggtctg ggcggagagc atagtagcta
     1261 cgagcagctc cagaggattc gaccagttca gctccgtggt ggcgccggga tactcttgtt
     1321 tgaggcgggg gatcacctcg gatgtcggcg cggctggcaa gatggacaga ttatatattg
     1381 gctagtgagc aagttccgct cacaagggat tggccggtcg ggatatccgg caaggtataa
     1441 tggggtccgg cgcaggcgaa gagaggactc tcacgttatg aagtggctca aggtctatgg
     1501 tctcccgaag ttgcccctct ttctcgcgcg atgggccatg aaactcgggc tacctgggcc
     1561 gacgtggtac aagtggaagg ctgcgagcgc ggggacgggc gtaatcctgg acgagatgat
     1621 ccgggccgcc gacgacctcg gatacgacgg gcagaaagtc ggcaagctcg ccatgagccg
     1681 catcggcgag aagcagggcc gcgacctgcg cgagcagctc ggcgtaaaga ccatgaagga
     1741 caccgtggac gtggtcatgc tcgccaaccg cttcttcgac atcgagatca cgctgaccga
     1801 gaccaaagac ggcgagtacg tcataaacgc cgaccgctgc ccctggttcg gcgggatggg
     1861 ccaggaaggg gtcatcggat gggacgtcaa accctgcgcc gcattctcca cctacgagaa
     1921 ggcgatggtc aacgccataa acccgaacgt caagctctcc tacaccgaga agcgaaccac
     1981 cggcggccac acctgccgcg gcgtctacca gtaccgcgac gcggagctgg gcgacggtcc
     2041 gcccgaaggc gtgcagccgg agatggtaag tattggcaag aagccgagta aggacgggta
     2101 ggagctttgg aacgcatcga gatagatacg gcgcgcggca aggagcgcgt accgccgggg
     2161 cagtacctcg tcggagaccg gtggccgata ctcacctacg gctcgacgcc ggccttcaaa
     2221 cccgagaact gggacttcgt gatggagggg ctggtcgaga atccgatccg gtttacttgg
     2281 gaggagtgga acgatctccc gaccgtggag gtcaaggcgg acatgcactg cgttacctca
     2341 tggagcaagc tggacaacgt ctggacgggt gtgcaggcaa agcacctgct ggagatggta
     2401 cggccgaagc ccgaggcaag gttcttctcg gcgttctgcg acggcgggta caccacgaac
     2461 ctgccggtgg aggaactgta cgaggaggac gtgctgtttg cgacgcacca caatggagag
     2521 ccgctggagg cgggccacgg cgctccgatg cggctcgtga tcccgcgcct ctacgcctgg
     2581 aagagcgcga aatggcttcg cggcctggag tttctggacg aagacaaacc ggggttctgg
     2641 gagcagaacg gctaccataa ctacggcaac ccctggcggg agcagcgcta cagctttagt
     2701 ttttagagcc ggtcggattt tagcggttgg atcccggcgc gactgcgtag tggtagcgct
     2761 tgagcagggg gagtgtcagc gccgtgacga gcgcgcggtc gcggggtttc atcatcttct
     2821 tccacgcctc gtcgggcctc agttcgacgg agccggtatc gaaacggttt gggtttccgg
     2881 agacggtgtg gctgatgccg agcttcacct cgcgctcgct ggctaatggt agttcggagc
     2941 cctcttcgcc gattagcttc aggatacgct cgaagctctg gcgggggttg acgacgaaat
     3001 cttcgtagcg gagtcgcagg tagcgttcgg gggtgcggcg ccagagggtc tcggcggcgg
     3061 cgttccagga gtcccagagg gcggcgctct tggcggggct gtggcggtgc atgaactccc
     3121 ggtcctcggt gtcgggctgc ggcttcttct tactccagga gtaggcggcg gcgcgggaat
     3181 cccggaccag gtgcaagaca tagagatcca ggcccggagc catccccacc gcgtacccgt
     3241 atgcgggctc cttcgaagag tccacgatga cgcggctccg cgctacaact ccgatggccc
     3301 ggtacagctt cgcggtgttg tcgagcagcg gccctattcg ctctcttatg gagatctccc
     3361 cctgccgggt cagcatggct gggatatggc gcgtgcgagc gccgagggac tgcaagcgca
     3421 tcatctcgcg ggcatccacg ccgtccatgc cgccgaacgc ctcatccatg accctggtcc
     3481 acaccgggca ctcgtggaat ggctctccgc agccgcacag ccggttctcc aggacgttgt
     3541 gcttccagat aaagttcaac tccccggcgg agaagaagcc ttcgatctgc ccgaggctgt
     3601 tggcgaggat ggtggagccg ctccgtccga gaccggccac gtagaggacc ttagtcccgc
     3661 cgctattcac cgccgctctc ccggttccgc gagccgagcc ggcggacgcg ccgcaggaca
     3721 cgcttcgcca cgttccagaa ggcgccgagg aactccctgt ccgtgtcggt gaggccgaag
     3781 agcgcgagca acgccagata gccgaggccg aagaccgtgc cgacgaggag tatggagggg
     3841 atagccggca tggggacgag catcccgacg acgtaggcga cggcggcgga gatcagtccg
     3901 gcggccaggg gtttcagcca cgcgaggttg tacggccaga agcccaggcg gcggcggacg
     3961 gcgagcaagg tggccgtgtt ctcggcgacg ataccggagg ccatacccac ggcggcgccg
     4021 agtatcttgg actccggggt gaaagccagc gagatcaggg tgacgctcac ggccaggccg
     4081 gtgagggcgg cggcggcggt ggcgaacata gctatgttct ggttcccggt catggcaagc
     4141 atgcgcggcg tcgggccgac cgaggaactg tagagctgcg cggcggcgac gataatgagg
     4201 gcgaggatac cgcccgcggc ctgctcgccg aagccgagca ccagcgcgtc cctcgccagc
     4261 agtatgatca ccaggaagat cccgaacgct cccgtgaaga tccagcggga tatgtccttg
     4321 tagagcagcc ccaggttctc ccggtccccc tgggcatgga ggctggagat tatcggagag
     4381 aagattcccg agaacgcgaa ccgcactatt gtggagaacg tcgccgtacg cgccgccgcc
     4441 tggaagacgc ccaccggagt gccagcggcg aagtagccga gcacccagat cgccgaccag
     4501 gtattcgcgt actgggcccc ggtggcgatg ctcatcggca ccgagaccga gaacagcgcg
     4561 cgggtctcga acttgggccg caccctccgg tcgaagagcg gtgggaagag cttcctcaag
     4621 aaatacacgg agacgacgca cccgagcgcc atcgagaccc cataggcggc gacggcgccg
     4681 atcacaccgg ctcccagaag gtagaagaga ggcacgagca ggaggaacag ggcagggcga
     4741 ataatctgct gcacgtagct ggcgtaggtc acggtctgga aaccctgcgt cgcccagagc
     4801 accatcatca tgaaggtgaa aaacggcaga gatacagcaa acgccttgag cacgggctcc
     4861 atggcggact tcttgtagta cagatccgcc gcgaagcccg ccccgaagaa catgacggct
     4921 gagagcgcca ggctcagcag cagggccacc atgacggact ggattatggt gccgcgaacc
     4981 cgtgccgtgt cctcctgggc gcggtagtgt gccacgtaac gcaccacgcc gttctccatc
     5041 ccgaagcgcg ccaggatctg cgctccgttg gaggcggcga cgcctagaga gtagaacccg
     5101 taagcctgga gcccgaacgt ctgggcgatg atgatctggg ccaggtatcc caggacgcgg
     5161 cctatcccct gcccggcggt gctgatgccc gccccccgcg cgacccgcgc cacgtaggtg
     5221 tcgtccttgt ctctgcccag ggtatccgcc gcggagacgc ccggaacccc ggacggcgcg
     5281 tctcccgccg cgtctggcgg gcgttcccgg tcggcagccg tctcgtccgg ggtcagggtc
     5341 tctccgggct cctggtgctc gaaatcgggg cttttactgg tcgcagcgtc gtcgcgccgc
     5401 gggttctctt ccatagtcac ggcgcagatt atacgggttt tggctcggac ggtaaggccg
     5461 gggagatcgt ggagttagta tcccgcgcgc ttcttgcgcc ggaaagatat aagggcttca
     5521 agcaacagcc cgaggccgat ccagacccag cagatgccat aggctaccca caacgccgcg
     5581 ccgacggtcc cgatatcttt cgagacggcc accagaactc cgaccgaacc cacggcgaac
     5641 ggcagcgaga tccagcgcgg cagcgtgccg ctccgcaggt tcgacgcgcc gaacaggatg
     5701 gacccgacgg cgaggaccag aagcccgagc ctgaaggtcc ggtaagcggg cgccagcaac
     5761 aggtaggtat cgtccacccc caaccagaac tcggccgtgt cgccgatgaa gaccaggatg
     5821 aggcccagca gagtgaccca gaacccgatc tcccagctta gccggtggcg gcctctagtc
     5881 cagaaataca ggccgaccag gccgaccatc aggaggagcg gaacggaggg gaagagcttc
     5941 cagaagacgt tcggcgtctt gtacttccac tcggagaggt ggacgcccag gggagagagc
     6001 gaccacagca cgcctcccgg cacgcagaag agcgcggtcc accggagcag ggtcttgagc
     6061 cagccgggca agactctacc ggctacccgg acgatccggc cccgcggaga tcaatacctg
     6121 ggtatcgatc caccggcgtc cctcagagcg tagtcgttga acatccgctg cacgatgtcc
     6181 cggttgtctc cggctatgtc ctgcttcata tccgggtcgg actggatatc gaagagcttc
     6241 gcctccgcgc cgtcgttgcg gctgatcatg acatagcggt cgtcgtgcga gcggacgtag
     6301 tcgttgtagc ccagggtgaa gtgggaccgc ggcgcggggt ccttgccgtc caggatcacc
     6361 gagaggtctt ccccgtccat cggatccttt tgctcgatgc tggtcgcgcc gagcaccgtc
     6421 ggcgccacgt cgtggatgga ggcgtagtaa tcgctggtct cccccgctcc cttgccctcc
     6481 gggtgccgga taaagaacgg gatgtccgta agttccgggt agagggccca gtccggcttg
     6541 ccggtgtcgt tgtgctcgcc cagggcgacg ccgtgatcgg agaggaccag gatcagggtg
     6601 ttgtccagca gcccgaggtc ggagacctta ttcaggaaat tgccgagcca gtgatccacc
     6661 atcgtaatct caccggagta caacgccttc atccgctcca gctcgcggtc cgtgatccat
     6721 ccggcatcac tgtagttggg gacgatgggg tctggtccgt cgtagtcgtc gctatagagc
     6781 ttcacatact tctccggtgg gtcccagggc tcgtgcgggt cgaaggagtc caccagcagg
     6841 aagaacggct ggccctctcg cgctacctcc aggtactctc ccgcgcggag gaacaccctg
     6901 ggcgcgaacc agtcctcctc ggtcttgcgg ttcatggtgt tcgccaggta ctggcggacc
     6961 ttgccccgca tgccgctggt gttgcctgag accgtgttgc ggtccaccat gtcgtcagag
     7021 acccttagaa ccgagtggta atggtccctc tcctggcccc ggatgaagtc gaatatgtcg
     7081 aagccgcgct ggaagttcat cgaggccttg aactcgtgct gggtgtcggt gatcaaggcg
     7141 gtgttgtaac cctggggcag cagtatctcc gagagggtgg tctgatcctc ggggattcgc
     7201 tgccagccgg ccgggaagaa ggtctctcct ttctggggca cccagttcct gaagggccag
     7261 gtgcgaattc ccgtgtggat ggcgcgccgc gccgggatgg tcggcaccga ctccgggtag
     7321 gcctgagtaa agcgcaggct ctctttagac aaggcatcga gggtcggtgt cttcacccgg
     7381 tcgctgccgt aggccccgat gtggtcctta cgcagactgt ccaggacaat caagatcacg
     7441 ttcatcctcg aacccccact cggaagataa ctgctgcagc ccgaaccaag cgccgaagtg
     7501 ccgagaagcg ccgcgctcgc cgcacccgct ccggccacct ttagaaattc tctgcgcgtt
     7561 aacgtactgt tcacccggct tcccattccg ctactcgctc tatcgaggct tgctatcggg
     7621 cgagcttcga tattgtcggc accgctcgcc aaactggccg ggacatattc taaaggatat
     7681 ttctccaacc ataggacaga gtctccacct gaagtctcct ataccggaac cacggctcaa
     7741 ccgcgcgccg gtgagacccc agagcgtctg ggtcggctcg tgaaccacgc gagggtggcg
     7801 cggagtcctt ctcgcgcagg tacgagcggc tcccagccca gggcctcgcg ggcgcgggtt
     7861 atgtccgggc aacgttggcg ggggtcgtcc tgtggcaggg gttcaaaggt aattttgctc
     7921 ctgcttccgg agagatccag gaccatgccg gcgacctcgc ttaccggata ctcgaccgga
     7981 ttgccgatat tgacaggacg aacctctctg gaacgcatga gccggatggt accctcgatc
     8041 aggtcgtcca cgtactgcac gctcctggtc tgacagccgt cgccgtagat ggttatgggc
     8101 tcactggcga gcgcctggga gacaaagttc gggatcatgc gcccgtcgtc gggacgcatc
     8161 ctggggccgt aggtgttgaa gatgcgcact atgcgggtgt ccacgccgtg atggcgatgg
     8221 taggccatcg ttatagactc ggcgtagcgc ttggcctcat cgtagacgcc ccggacaccg
     8281 atgggattga cgtttcccca gtactcctcg ggctgcggat ggacgagggg atcgccgtag
     8341 acctccgagg aggaggcgag catcaggcgc gagcccttgg cgagcgcgag gccgagggcg
     8401 ttgtaggtgc ctaaagcacc agccttgagg atcgggatcg ggatgcgctc gaagtctttc
     8461 gggctggcag gcgaggcgaa atggtagacc tcatccagct caccctcaac ctcgatgtga
     8521 agtgtaacgt cgtggtggat atactcgaag tccggcgagt cctgaaggtg agcgatgttc
     8581 tcaagagatc cggtacgcag gttgtccatg cagatcaccc ggtatccttc gccgagcaat
     8641 cgctcgcaca ggtggctccc caggaacccg gccccgcccg tgacgagcgc gcgcggcttc
     8701 accaacttcg ccttgcctgg atcgctctac tcacgtccga agccccggta gagcagcccg
     8761 cactcgcgga cggcagcggg gtcgaagacg ttgcgaccat caatgagaag ccggggctcc
     8821 tccatcaggt cggcggcccg atcgagatcc aggctccgca cctcctccca ctccgtcacg
     8881 actaccgcgg cgtgcgtccc ggcgagtgcc tcgtaggggt cgaagacgac cttgaggtcc
     8941 ggcattagcc tggccgcggc cttaccggcg acggggtcgt agccgacggc gcgggcgccg
     9001 cgggcgtgga gggcacgggc aatctccagg gagggggcct cgcgcaggtc gtcggtgttt
     9061 ggcttgaagg cgagaccgag gagggcgata cgcttgccct tcagggtgtg caggtcgcgc
     9121 tggagcttgg agatcacctg ccgccgctga cgctcgttga cggttacggt ggcgtcgagg
     9181 agggccggct cgtaatcgta ctcgcgagcg atggagcgga gggccgcgac gtccttcggg
     9241 aagcaggagc cgccccagcc gataccggcg ttcaggaaac gggccccgat ccgctcgtcc
     9301 aggccgatgc cggtggcgac gttcgagacg tccgccccga ccagctcgca gatcgcggag
     9361 atctcgttga taaagctgat cttggtcgcc agaaaggcgt tggcggcgta cttgatcatc
     9421 tcggcgctcg ccaggtccgt ggtgacgaac ggcaccgccg cccggggccg cgggtccaca
     9481 tccgtcggga agctctgctg gatgatcggc tcgtagagcg cccgcatcgt atctaacgcc
     9541 tctcgcgagt cggcgccgag cacgatcctg tccgggaaca acgaatcgta gaccgcgctg
     9601 ccctcccgca agaactccgg gttggacacg acgaggaact tggtccgcac ggtagctacg
     9661 ccttcggctt cgtcaccggc cccatcctcc gcaccttcct cgatgagcat ggagacataa
     9721 tccccggaac cgaccggcac ggtgctcttg ttgaccacga cgagcgggcg ttccctctct
     9781 gtctccgaga gagtcttccc tatgctccgg gcgaccgccg ccacgttaga gaggtcggca
     9841 gagccatctt cgccctgcgg agtattgacc gccacaaaga ccacgtccgc cgagcgcgca
     9901 acctccgaaa ccccctccgc gcccgcgaac gagagcctgc cgcgccgcgt cccgccggaa
     9961 accaactcgt cgagccccgg ctcgtagatc ggcacgcgcc ccgctacaag ctccgcgata
    10021 cgctcctcat cccgatccac gcacgttacc cgatggccga gataagccag gcatgccccg
    10081 gtcacgagcc cgacatagcc cgtacctacc acaccgacgt tgaagctctc agtcaaaaca
    10141 gcatccccca aacaaatgtc gagagaagat cagatacatt gccgcccaca atctgcactc
    10201 cgatagctta ctagaaacat ctctttgcgg agcatgacag ccaccttgcc ggagaaacat
    10261 agtccatatt gcgtatagct agtcaccgtt ggagcaagat aaactgtgcg gattagggaa
    10321 gggcgtattt aggcagtttc aaggaggtgg tacgtcaggc aatacgcgac gagaaatatg
    10381 caagaaacat tccaatcaag agaaacgcac ggagaggagt agaggatgag aaagctgacg
    10441 atattggcgg taatgctgac gatggcgctc atggcggccg ctccggcctc ggcagataca
    10501 gtgacggcag acggcctgat cgcgggtacg ctcgaaggag gcgtggtggt agccggtaac
    10561 ccgaacctcg ctaatggcgc ccctactcta tccctcggcg acgatgtggc cgcggccggt
    10621 agccaagttc cgccagatgc tttcgccggg ggtttcaaca taccatttac ggtcaacgtc
    10681 gcagacgccg gggtgggaac gacaatagaa gacgacgtca tcgccggaag cgccttcctc
    10741 gacaccaact tcgttgaggc cgggtccggg agcctctctg ttgacacaca agttggattt
    10801 cttgactgag aagtaatcgc aagtcgacgg cgatgcgagg gtgggccatc atcccaccct
    10861 ctcttcccgt cagtatgcgc cctcacgccg cagcacggct cccagagtcc tgagtatgat
    10921 cttgaggtcg aaagagagcg accagttctc gatgtagtag aggtccaggc ggaccatctc
    10981 ctcgaacgag aggttgctcc ggccactgat ctgccagtac cccgtcatcc ccggaatcgc
    11041 cgccagccgc cgcttgtgca actctcccat ccgctcgaag tcacgcagcg gcaacggccg
    11101 cggtccgacg agactcatct cgcccttcag gacgtttatg agctgcggaa gttcgtctat
    11161 gctccagcgc ctgaggaaac gtccgacggg cgtgacgcgc gggtcccgct tcattttgaa
    11221 gaccggtccg tcggcctcgt tcagagcctc cacctcctcc tgccgcgact cggcgtcctc
    11281 gtacatcgag cgaaacatgt agcagataaa gaccttctcg tccacgccgg cccgcttctg
    11341 ccgaaagaag gccggtcccg gagaatccag ccttatagcg agagagatag caaggaagag
    11401 cggcgagagg aagatcaggc cggccgccga gagtgttatg tcgagggctc gcttcatggc
    11461 gcgctgggtg ttgtcgaggc gcgggtagcg gacctctaga agcggcagac ccatgctctg
    11521 agagaggatg ggctggttgc gaagcaaggt tagggcgccg ggcacggccc gcatccgtat
    11581 gccgcgcaac cgcacggagc gcatcagatc tagcaggtct tcgtcggcca ggcgctcggc
    11641 gcccgcgagt atcaccccgc gcgcctgggt gccatcgagt agctggcgta gcgcggcgag
    11701 gtccacgctg tcctgcagat caatctcccc gactaccggg taggcgccgg gagcgtccag
    11761 catcatgcgg cgaattcgct ctctttcggt ggcgtttcct atgatcacca tgggggcgag
    11821 agcgaagccg cggcggtaga ccatctcgat cccctgctcg tagagcagcc tgagagcccc
    11881 gctaacgagc aacgacagag acgcagccac gacgaccccg gcggcgcgca gtccgctctc
    11941 cgggtagatg gcgactcccg cgagggcgat cccagtccat gagagcgtcg ccgcgagcag
    12001 ggccccaggg ttgcgccgga ctcgcgcccg gtcatagagg tgaaacgcgg ccagcagcgt
    12061 gatccagacg gcgagcagcg ccggcgcgag atacagcgcc tgatcaagag aggtttctcc
    12121 ctcgatcagg agagccgagc ccgcgagtcc cagcacgaga gccgccgcgt cggcgagcag
    12181 cagggcagat acgctcagga tacggcgcaa ggtctttatg tagagcaaag tcttcaagag
    12241 gccccggaac caagtatacc gccgggcaga ggagcagacc gccaggcgga ggttctacca
    12301 gctcccgcgg gagtaggtct ggtccgggac cttccagccc ttcggataga cgaggtaggc
    12361 cacgccgccg gaaccgcgcg tcagccaggc gtcggcgaac tgatcgcgct ggtatacgcg
    12421 ccggaccccg gagttgctgc tggctgccgg gtcgttgacg ataacgtact tgccagtctt
    12481 gtcgaagccc cggatcacga ccagatgccc gttgctcgcg gggataggag cgccggcgag
    12541 ctgtccgctt ccccaggata tgctcgcgac caccggcacg cccgaggcga tccatcgctc
    12601 cacctgcccc agcgaggaga aacggttgac cgaagctcgt agcccatacg ccgaagcgta
    12661 acccgtgttg aacggccagt tgccgttgcc ccggtatgtg tagtcgtagg tgcccgcggc
    12721 caccgtcggg accggttggt tcaggctccc ctgtccggtc cggttcgacc agtaagccat
    12781 caccatcgag agggaggtcg ggctgcacca gacctctcca ccgttcgggt agatcatctg
    12841 cgagcgctcg ggcaccggta ggtccttgcc ccacaacgcg ctactccccg caacaccgag
    12901 gaactccccg tgccggtaag aattcgagac ggtgaaggct agctggcgca cgtcgggaga
    12961 cgctccgctc ttcttgctgg agagcttcaa ccggtactgg taagcatcgg cgaagatagt
    13021 gcccttgctc tgcaacgtgt cggtcaggac ctgccagttg gtcgtgctct ggccgttgac
    13081 gctgtggcgc ttgatcgtct ccgtccccct ggcccagacg cccatgttga accacggagt
    13141 ccagttaccg ccggaacgga cgcggacctc ggtctggata taggtgccgg cgggggttgt
    13201 cgcgttccag gaagggagga gagtgtcgaa ccgggctgag gggagatagg cccgtgaggt
    13261 aagggtgccg gagcgtacac cgggacggag acgcaccgcg ccggccccgc cggagagctt
    13321 cttcacgtac actccgctct tcgtccccga cgcgagcgcc ttgtaggtcg agtgacggtc
    13381 gaagtctatg taggagctca ccgcgccggc ttctccagga gtcaggagca tcagacaagc
    13441 gaacgcgagc atcatcgcgc ccgtaagtac cggcaacact tttctcgaat acggtcccct
    13501 cacggagaga gattctatct taccgcgccg gcccgcgtct tccttttcct gagatgcgtt
    13561 gtctcaggac gcgcgcgagg aagagagggt tcccgatcag gtaccgccgc cacagtcgtc
    13621 ggggctcgat gaggagccgc gccagccact cgaacccgtt gtccgtgagt agacgcgggc
    13681 cgcgccgcaa ctcgcccgcg acgtagtcga agacggcccc gccggtgagc gcgaccctcg
    13741 cgttcagcct gtaacggttc tccatcagcc agcgctcctg gagcggcata ccgaagccca
    13801 cgatcaggat gtccggcgcg gcggcgttta tctctcgcac aaccatctcg ttctccgagg
    13861 ctccagtagc atggtcgaag tggccgtggt gcgcaccggc gatccgcagg ttcgggtagc
    13921 tcccggcgag ccggtcggcg gcccgctcca cgacgccggg gcgggctccg aggaggtaga
    13981 gggagaaacc ttcctccgaa gcgagggcgg ccaagcgcca catccaatcg gcgtaggtga
    14041 tcctggccgg tatcctgctg cccaggagcc ttgcagcgag cctgaccccg gccccgtcgc
    14101 agaacaccac gtcggcgcgg ttgaagcagt cccgcaacgc ggcgtcccgg caggcaaggt
    14161 tcagggcgtg ggcattgacg ttcaagacca cggcgcgcgc ttcgcttcca gccaaccgcc
    14221 ggaccaccgc ttcgagttcc tcggcggtca acgggtccac cccgaccccg aggacgtcca
    14281 cccggtctac agggcgcaaa ggatcttctc ctcaagatcc gcgaagcgca gctcccacga
    14341 gttctcgcgc gcgagctgct gtctcgcgcg gcgcctctcc tcgttctccg aagccgcgat
    14401 ctcccacagc gccttcacgt agtcctccgg tctctcggca aggtagacgt agtccgcgag
    14461 ctccatcatc gccgggagcg gcgccgcgac gacgggccgt ccggtggcca ggcattcgta
    14521 ggtcttggca ggcgatatgc ctttcgtcag cgcgttcatc cggtagggca gcacaaaggc
    14581 gtccacgtcc gcgaaggccg atggcagatc agcatgagag acctcgcccc gataatctat
    14641 gccggggagt ttcaagaaac cccgctccac gcgccccata ccgccgatga tccgcacctc
    14701 gaagcccgcc cgcgcgatgg tccgcagcac cgcgaagtct atccgctcct cgccgaggtg
    14761 gccgaagaag cagacggtcc gtatgtctgc gcgccgcgag ggaacggtgg cgaagcgttc
    14821 gtagtcaacg ccgggaccgc tgaggaaagc gtccggccgc tcgggacgca ctttctccag
    14881 caggtgggtc gaggtgcagg agacgaggtc cgaccggcgg agaagctcgt gttcggtggc
    14941 ggctatgtcc ttcgggacgc cggggaaggc cgcatagtcg tccgagcaat cgtagaggac
    15001 cagacgaggc cggaggccgg agatcaggtc aagcgtcgtc cgggtcggcg ggtaggcgat
    15061 gatcacaggc tccggcccgg ttgacttcct aaggtcctcc acgacacgcg gcacgaagaa
    15121 cctcgtgttg agccgccgaa atatcttcag ggtcggcggg agcgtgagcg gcgagtagac
    15181 ggcaaggttc gcctcggaag acggtatttt gctgccggat gagcgccggg cccttgcgag
    15241 aagcttgcgg agcgtggctg gtgcgggctt cggggttgcg aggccggtcg tctcgacgaa
    15301 ggtggtcggg tagccggcgc gagcgaagag ggtggcgagg gtttggtgac gctgccacag
    15361 gaagtcccat cggacccccg cgaggattat tacgggcggg cggtccaacg gctcgtctcc
    15421 cccgccctcg aacgcatcat ctccctcgcg gccccggtgt ccggcatgga gtcctccccg
    15481 tagacccggt cgagcccgaa gagcgccagt atctctaagg ctcgccgcag ttggtttcgg
    15541 tctatccgtt cacgccagtc gtccggcggc tggcgcatgg acgtgttccg gctaagtggc
    15601 gagggacgtt tcatcttctc gtagaccctc tcgtcgagat ctacgccgag ggcgtcgaac
    15661 agccggggga tctcctcttc gggacgagct accaggtttt cgtagaagac cagatgcgcc
    15721 tcgttctggg agagctgcat caggggcacg tagttgtcta cgcaccagag gaacaggtgc
    15781 cgctcgaacg gcgtcttcgc ggaccgtatc ccgccttcca tcggacgcag gaagtcctcg
    15841 acgaggtcct cctgccgcat cgtctcggag aggttgtccc gccatccgag ggccatcctg
    15901 gaggcggcaa cggcgcaagg atggcgcagg aggaggacca ttggcatttc ggggaagttt
    15961 gcgtggagcc agccgaggag caggttagcc cttatgtcct tgacgagccg ccgccgcgcc
    16021 aggaagctcc ggttgaagcg gtccgtccac gcgctgcgga ctccgcccga caggatctta
    16081 cgcgccgggt ccaaaaactc ctcccgccgg tcgtccggcc gaaggtactg cttgctgtag
    16141 aagtcgcggc agatcccgac tctctccggg ttgaacggct cgaagatgaa gcgatactcc
    16201 cgacggtaat tgatgatgtc cgccagccat gtagtcccgc ttctgccact cccggccagg
    16261 aagacagtgt tacggtagtc ccggttcagg tccaggtaga gaccgccgag caaccgctct
    16321 ctcaatccgc tcatccgaat ccgttcggaa ccgctcatcc ggtcttttct ccgggtcccc
    16381 tgggcgaagt ctgccgcgcg tgcctgccga gcgacgcccg gaaagaggcc aggaactgcc
    16441 ggtcgctcgg gctgagccca agagagagga gaagcgccgc gaacacggcc agagccactg
    16501 gagcgcagac cagcagcgcc gggagcccgg cttcgagaag gatagagcga accaggtaga
    16561 gcgccgccgc gctggcgacc cccgccagca aaggtttcgc gtaccggcgg ctgtagggcc
    16621 agaagccgag tcggcggcgg acgaagtaaa gtgtggcgac gttcgacgca acgagagccg
    16681 cagccgtggc ggccgccgcg ccgaggatac cgaagggcgg caccagcagc aggctcgccg
    16741 cgacggcgag tagtgccgag cccacggtcg agaccatcac cgccttctgg tgtccggtca
    16801 tcgccagcag ccgggctgtc ggaccgacgg aggagttaaa cagttgggcg gcggctatca
    16861 ccacgatcac cgtccacccc tgcgagaact cttcgccgaa gaccagcatg acgtcccgcg
    16921 agagcatcgc cgtcacgaca aagataagga gagctccggt aaacgcccag cgggagacat
    16981 cctcgtagag atagccgagg tcccgcgtaa gtccctgctg gtgcaggccg gaggccatcg
    17041 gagaaaagat gccgccgaag gagatcagga cgagcatcga gagcccggcc gttcgggccg
    17101 ccacatcgta caccccgacc acgggagcag gctcgaagac gcccagcacg atcacggccg
    17161 tccacaggtt cacgtactgg gtgaggttgg cgacgatcat tggtcccgat accgcggata
    17221 gctcccggct ctcgtacctg ggccggacgc cactctccaa caactccggg aaaaccttct
    17281 tcaggtaata gagcgccgca accgccccga gaaccatcga gagcatataa gccgcgacgg
    17341 cgcccaggat ctcgacccca agcagataga agatgacgac gaggatcagg ttagccaggg
    17401 gccgcagcac ctgctgcacc agcgtcgcgt acttgaccgt ctggaatccc tgcgtggccc
    17461 agagcgtcat gctcatcagc gtaaagaacg gtatggagac cgagaacgcc cggaacatcg
    17521 tctccaggaa cggcttgccg aacacttcct ccgccaggaa ccccgctccg aagaagacgc
    17581 caccggagag catgatgctc agagcgaacg tcacgccgag agcctgaagg atagtgccgc
    17641 gcacccgcgc cgtgtctccc ccggccccgt agtgggctac ataccggacg actccattat
    17701 ccaggccgaa ctgcgcgagc acgttgacga cctgcaccag cgtcaccccg agagcgtaga
    17761 agccaagctg cgccggtccg tgcatgcggg ccagggcgac ccttgtcgcg taacccagga
    17821 gacgccctac accctggccc gccgagctga cgccgacgcc gcgggcgatc cgggccacat
    17881 agtcatcata gccatcatcc ggcgaagtct tccgggtgct cagggatcca tcctcacaca
    17941 ccgggttgcg tttcgctctc atcgcgcccg atgatatgct ctgccccccg ctgcttcaat
    18001 tgaacgcgca gagagatgcc gtctactgct tccctacggc cgggcctcgc ccaccttcag
    18061 caatgatttc ctccgaggtc tcccaatcct tctcggcggc gagcttcacc gcgatagccg
    18121 cgaggataaa gaagaacagg gtcgagggcg ggatgtacca gtgccactcg atcactgacg
    18181 atacgaggta gaccacggcc gccgccacca gtcccgagag caagacgcgc cggtctcccc
    18241 gcgcccccga gcgccaggcg gccctggcag tgtaacccag gagcagcgcc acgaagctca
    18301 cgagcgccag gaaagcgaag atcccggtct ccgtgccttg ctccagatac aagttgtgca
    18361 cctgcttgac gccggttgtg ataggccgat tcttcagcca gacatactgg aacgtgccgg
    18421 cccccgaacc ggtcagagga tgctccttcc actcgtccca ggcgactttc cagtagtctg
    18481 cccggaaccc gatgctggcg gacgcgagcc tctggctggc gttcttcgtg ttgaccgggt
    18541 tgttgacgag agcttggtac gtcttctcga ctcccccgta ctggcttacg accacgaatg
    18601 ccgccgccag cccgaccaac accaccccgc caagcgcgag agctccgaga gtcctgcggc
    18661 ccaggggcag gagctcgtag cgactaaaca ccagagagta acccgcctgc agcacgaagg
    18721 ccacggcgag agcgatgatc aggtagtcgc ggaacgccgt gccgtcggcg atcttctgct
    18781 cgttcggcac gagagaccag agtcctttca ggtccttcat ctcccagaac agccacgctc
    18841 cggggataga gaccagagcc aggttggcga agatctgcaa cctgtccgat gtcaggatga
    18901 agagcgccat gagccccacc ccgagcgatg cgatgccgcc acgggagaca gtcagcagga
    18961 gcgccacgag aaaggcgagg acaaacacgg cgtacaaccc tcgcaaaacc gcgttccgca
    19021 accgcgccat ccgggccagc gccagcacga cgcccatgcc gagcaccatg gcgaacgtgt
    19081 tgacgtaatc cagggtcgaa tcgatcctca ccttgtccag cgaggtaacc gggtactgga
    19141 ccgggctgat cttctgcatc aggccgtagc ccgcgaccgc gaccactata agcaccgcca
    19201 cgtccatcag gggcgccacc tgccgtcccg aattgagcgc gccgagcgcg agcaagaacg
    19261 ccgccacata catggagcac cgcaggaact ccttgatagt ctccgtctcg ctcaatgtcc
    19321 acgtaatgga gacgcccttt atagccgcga gcgccaccaa aagcgcgaag agggcccacc
    19381 ccgcagccgg cacatccacg taatatcccc tgacgaagac cgttaccagc agcagcgcca
    19441 ggatacctac ggccaccggc agccagaggt catccgagta gagaccgtcg ttgatcatgc
    19501 cgtatgccgt aacgcccagg agcgccagcg ccaccagcgc cttgaacagc atcaacatcc
    19561 gtcccggacg ccggacctta tcccggccgg gctcacggtc ttccttactg aaggtccatc
    19621 cggtttcctc gctcaaacgc tctctccggc tccattgagc accactccca ggaggtttac
    19681 accggcgtcg agcaagtcgt ccgtcacctc gcgagcagtg tttttggaag tgcgcgaagc
    19741 gtggatcacc agcagcaccc cgtcgaactg gctggagagc gcgggcgact cgatcacttt
    19801 ttcggccaca ggggcgtcca gtatgacgat gtcgcgatgc gcctcgatac ctcgcacgac
    19861 ttctacaaac ctctgtgact cgatgcgggc cacggggtcc gaagagaccg gaccggtcgg
    19921 gacggccagc aggccgggga cgaggtcgtg cccgtaattt tccagggcac ggccgctctg
    19981 taagcagcta gtcagcccga tgaagttcgg ttctccaagc atccggtgca ggttcggccg
    20041 gccgaggttg cagtccacaa ccaccacccg ctttcccatc tccgcgagcg ccgctccgag
    20101 acctatgcac acgctagagc atccggcccc cgtttcgggg cttgtgacta ccatgctcct
    20161 cacgcgatcc tccagagaga ggaggttctg cgccagcttc gcgtaatgcc tcctcacggg
    20221 tgaggcggga tccaaaagca cctccacact accttctcgt atcgctccca ggctcagatc
    20281 acacctcctc cgcggaagga tactccggaa tgaccccgag caccggcgcc cgcagggtca
    20341 actcggcatc tctcgcgtcg cgccaactgc gagcccggtt ctcaagcaag agcgccatcc
    20401 cacccccgat caacattccc gcgactatag cgcccgcgac atagaagagc ggcccggcgc
    20461 cagaggggcg atccggtagc gaggctttgc tcgctacccg cgcatcggcg gcgagagcgc
    20521 ctcccgcgag ctgccgatcg ttcagcaggt caaccctttc ggtgaacagc gccgcgtagg
    20581 cgttagccgt gcgggccgcc tcctcggcgt ctacgctctc gaaccggacc cggattgtcg
    20641 gcgttccatc ggaagtaacc agggactgca cgtcgagccg tcttgcgaac tcttccggcg
    20701 ttcccttcca gccggcctta cgcctgacat ccacgataaa gtcttccgaa gaggtcaccg
    20761 ctcctgtaac gtcccgaagg aacggttcca tatcttcggc gggaacctcc tgctgcggtt
    20821 cgaccaggac tatagcctcc gcagtgtacg cgcgttcgcg caatagcgcg aacgtcaggc
    20881 ccacgatcag aagagataag acaatgatgg agatcaagcg tctccatccc catagaacgt
    20941 gcaggatctc cgggagcgcc aggtcattgc gtcgcgctct ctgccatcct tgccgtccga
    21001 tgcggttaat agatctcact gttcaccacc gaatcccggt aggcctcggg atctgaactc
    21061 aagagctcgt ataacgccgg agcctgatcg gacgaactct ccgaaatcac ctttaatgct
    21121 cgattgtatt taccttcgac cacgagagca tccgcgagcg ccaagtccat ggattggatc
    21181 aggtcctctc tctcggtctt cgccgagcct ttgagttgct ccaacccctt ggaggacctg
    21241 cgtcgggcct gcaatatagc cttgtatcct tcctttggct caccagtccg cacatagatc
    21301 ctgccgagat catagagccc ctggtcggtg atctccttct cttccctgat cttctcgtat
    21361 tcttttttcg cctcttctaa cttcccctgt cttatgagcg cctgggcgag gccgttgcga
    21421 gcgacggtag cacggggatt tagctccaga acctgccggt aactcctggc cgcgccctcc
    21481 agatcgttca gcttgaccat ctgcaggttg ccgagcatga gatgaggcat gtagttgtta
    21541 ggatcccgct tgacagcctc ctgaagggct tccgccgcct gatcgttgag accctgctgc
    21601 tgcaaaagga acgactgcgc ctccagcggg tccgtatcga aaggatccag acgtatagcc
    21661 ttctgggccg cttcctccgc gcccttcgta tcacccgccg cagccaaccg ccgctgctca
    21721 tcagtgtagt ggttggagag aaacaacgcg ccagcgatca ccaccagggc gcccacgaga
    21781 aaaccagcgg atatcttgaa gacaatacta ctcaaactca tatcaccgcg catgagcata
    21841 tccacaacgt tatctaaata caacctgaaa gatgccccat taccagattc caggcaagag
    21901 aaagaggcgg acccccgaag ggacccgcct cctgtgtgct acaggtgttg cccggttagc
    21961 tagtgcttac cgaacgatcc tgcgcgcaac cagaccaccg gcaaccagca gagcgccagc
    22021 gccaagcgt
//
LOCUS       DASSMB010000185         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_804123, whole genome shotgun sequence.
ACCESSION   DASSMB010000185 DASSMB010000000
VERSION     DASSMB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804123"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VFJ72_11015"
     CDS             <1..402
                     /locus_tag="VFJ72_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7480032.1"
                     /translation="GELNTFKVRHAGFAILESTTPPGGGMPPHVHHSQEETVYVLEGE
                     YVFFSGEEELVVGPGSLVSISRGTTHAFKVTGQEPGRCLMILTPPGPLERFFEEVGVP
                     VGNGASSGSPEMEEVLASARRNGIELLITPV"
     gene            438..1256
                     /locus_tag="VFJ72_11020"
     CDS             438..1256
                     /locus_tag="VFJ72_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480033.1"
                     /translation="MKRAVVLQMRSTRRPLVAAVVAVTLGLSVAAAGTVYAASAQQTI
                     NGCYGKFGGLLRVVKSPSQCHRGETKISWNQRGPRGVQGKPGPRGEQGTKGDPGPQGE
                     KGDKGDPGAQGIQGEQGPQGEQGPAGADGAVGPQGPQGPQGPRGPSNAYTDGPNDVSI
                     DTQDVTVASLALPAGNYVVSATLGLSNPSTSKVGLVRCALQPNDASTFYDAALEPFSL
                     NNPGADTLAFTQPVRLSSAGDVSVSCENFVNNGEKINGRDIVLTALQVESLAGQ"
     gene            1293..2612
                     /locus_tag="VFJ72_11025"
     CDS             1293..2612
                     /locus_tag="VFJ72_11025"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7480034.1"
                     /translation="MNRGTRNGDLPARLTADRARRGGGKARRIAALAAALLLALGVGV
                     SARPQPASAANGLFPILDCVTYDSANNFVTAYWGYSNVNGVPITPDFSDNYFDPPPLY
                     RGQPLDFQPGTYHKVFSATFSAGANNEGYLAWTLYGTTVVAKNDPNIYCEGADTVPPN
                     TAIISGPTGTASNASATFELFASESGANFECKLDDGDFAACDSPKELTGLSDGTHTFQ
                     TRATDAAGNTDPSPATRTWTVDTTAPGAPTITGPANGAYDRDGSFNVRGAAEPGSTVR
                     LYEGATRVGTSETDPTTGAWSVAVVDVPEGSHAYKARATDALGNVSGDSAEVTVIVDE
                     TAPSVTGVSPTGKKVSPAANITATFSETMDPSTLVPSTVDLTKKGASRPIPAQVTVSP
                     DGKKVILNANRDLRRGATYTARITTGARDEAGNALASSKVWTFTVRR"
     gene            2821..2970
                     /locus_tag="VFJ72_11030"
     CDS             2821..2970
                     /locus_tag="VFJ72_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480035.1"
                     /translation="MRQNLDLSREANPDPDTVGQPLTFNIKDTNTSDTITAGTNVIDR
                     ACRPA"
     gene            complement(2977..>3333)
                     /locus_tag="VFJ72_11035"
     CDS             complement(2977..>3333)
                     /locus_tag="VFJ72_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480036.1"
                     /translation="LDVTDRARMREVVERFAPLREDPAILVYNAGISPVYASAEKVDE
                     GDWYAIIATNLTGASVVPRGVEHQPVACEEVRRRDHDDLYRSRLSGMEIKSAAGETVP
                     ALFVHRVGRRTRWTSP"
BASE COUNT          660 a         1085 c         1102 g          486 t
ORIGIN      
        1 ggagagttga acaccttcaa ggtgcggcac gcaggtttcg ctatcctcga aagcactact
       61 cctccggggg gcgggatgcc gcctcacgtt caccactctc aggaggagac ggtctacgta
      121 ctggaaggcg agtacgtctt ctttagcgga gaagaggagt tggtggtggg accgggttca
      181 ctggtgtcca tctccagagg gaccacccac gccttcaagg tcaccggaca agagccgggg
      241 agatgcctca tgatcctcac gccgcccgga cctctggagc ggttcttcga ggaggtcggc
      301 gtgcctgtcg gaaacggagc ctcatccggg tcgccggaga tggaggaggt cctggcgagc
      361 gcgcgcagaa atgggatcga gctgctgatc acgccggtct gaaaggagtg ccaatggcac
      421 ggcagaggag caatagggtg aagagagcgg tagtactgca gatgaggtcg acccgccgcc
      481 cgctcgttgc ggcggtggtg gccgttacgc tcgggctgag cgtcgccgcc gccgggacgg
      541 tctatgcggc gagcgcgcaa caaacgataa acggctgcta cggtaagttt ggcggattgc
      601 tcagagtcgt caagagcccg agccagtgtc acagaggcga gacgaagatc tcctggaacc
      661 agcgcggacc acggggcgtt caaggaaagc ccggaccgcg gggcgaacag ggaacgaaag
      721 gcgatcctgg gccacaaggg gagaaaggcg acaagggcga ccccggagcg cagggcatcc
      781 agggcgagca gggtccgcaa ggcgagcagg gtccggcggg cgccgacggc gcggtgggac
      841 cacagggacc acagggacca cagggtccgc ggggtccgag caacgcctat accgacgggc
      901 cgaacgacgt ttctatcgac acacaagacg ttaccgtggc cagtctcgcg cttccggcag
      961 gcaattacgt ggtcagcgcc acgctcggtc tcagcaaccc gagcacgagc aaggtggggc
     1021 tggtcaggtg tgcgctccag cccaacgacg cctcgacctt ctacgacgcc gcgctcgagc
     1081 cattctctct gaacaacccg ggcgcggaca cgctcgcctt tacccagccc gtccggctct
     1141 cctcggccgg ggacgtgagc gtctcatgtg agaacttcgt caacaacggc gagaagatca
     1201 acggccgcga catcgtgctt acggcgctgc aggtggagag cctggccggc cagtaggttc
     1261 tcgaccggaa agcttattga aggagttaaa ggatgaacag gggaacacga aacggcgatc
     1321 ttccggcgag gcttacggcc gaccgggctc gtcgaggagg cgggaaggca aggcggatcg
     1381 cggcgctcgc ggcggcgctg ctgctcgcgc tgggagttgg cgtctcggcg cgcccgcagc
     1441 cagcgagcgc ggcgaacggc ctcttcccga tcctggactg cgtcacctac gacagcgcca
     1501 ataacttcgt gaccgcctac tggggttact ccaacgtcaa cggcgtcccg atcaccccgg
     1561 acttcagcga caactacttc gatcccccgc ccctgtaccg ggggcagccg ctcgattttc
     1621 agcccggcac ctaccacaag gtgttcagcg cgacgttctc tgccggggcc aataacgaag
     1681 gttaccttgc ctggaccttg tacggcacta ccgtggtggc gaaaaacgac ccgaacatat
     1741 actgcgaggg ggccgacacc gttcccccga acacggccat catctcgggt cccacgggga
     1801 cagcgagcaa cgcctcggcc accttcgagc tgttcgcctc cgagtccggc gcgaacttcg
     1861 agtgcaagct cgatgacggc gacttcgccg cctgcgattc gcccaaagag ctaaccggtc
     1921 tctccgacgg cacgcacacc ttccagaccc gggcgacgga cgccgccggc aacaccgacc
     1981 cgagcccggc cacgaggacg tggaccgtgg acacgaccgc ccccggcgcg cccacgatca
     2041 ccggtccggc aaacggcgcg tatgaccggg acggctcctt caacgtccgg ggcgccgccg
     2101 agcccggcag caccgtcagg ctctacgaag gcgcgacgcg ggtcggcacc agcgagacgg
     2161 accccacgac cggcgcgtgg agcgtagccg tggtcgatgt ccccgaaggc tcccacgcct
     2221 acaaggcgag ggccacggac gccttgggta acgtctccgg agactcggcg gaggtgacgg
     2281 tgatagtgga cgaaaccgcg ccctcggtga cgggcgtgag tccgacgggg aagaaggttt
     2341 cccccgcggc caacatcacg gccaccttct ccgaaaccat ggacccatcg acgctcgtcc
     2401 cttcgacggt ggatctcacg aagaagggcg ctagccgccc catcccggcc caggttacgg
     2461 tgagtcccga cggcaagaag gtgatcctga acgcgaaccg cgacctccgg cgcggggcga
     2521 cctacacggc caggatcacg accggggcga gggacgaggc gggcaacgcg ctcgcaagta
     2581 gcaaggtctg gaccttcacg gttaggcggt aggcttgcag ggtcatcgct ttccggcgcc
     2641 cccggccttc cccggcggcg aacacgaggg gagattggag caaggacgga ggcagagtac
     2701 cgcctctccg gagggttcaa ggtccgtagg aagtaggtgc gcgcagaagc cgcacacccc
     2761 ctcccgtttc tcccggtatc cgaatatggc gctaccagga gcattgagaa ggaactcgag
     2821 atgagacaaa acctggacct ctccagggag gcgaacccgg atccggacac cgtaggacag
     2881 ccgctcacct tcaacatcaa ggacacgaac accagcgaca ccattaccgc cggcaccaac
     2941 gtcatagaca gagcttgccg tccggcgtga ggttcgtcag ggcgaagtcc agcgggttct
     3001 tctcccgacc cggtgcacaa agagcgcggg caccgtctca cccgcggcgc tcttgatctc
     3061 catcccggac agacggctcc ggtaaagatc gtcgtgatcc cgacggcgga cctcctcgca
     3121 agcgaccggc tggtgctcca cgccgcgcgg caccacggac gctccggtga gattggtggc
     3181 tattatggcg taccagtcgc cctcgtcgac tttctcggcc gaagcgtaca ccgggctgat
     3241 gccggcgttg tagacgagga ttgccgggtc ctcccgtagg ggtgcgaatc gctcgacgac
     3301 ctcgcgcatc cgggcgcggt ccgtcacgtc gag
//
LOCUS       DASSMB010000186         3837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_162715, whole genome shotgun sequence.
ACCESSION   DASSMB010000186 DASSMB010000000
VERSION     DASSMB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162715"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFJ72_11040"
     CDS             <1..211
                     /locus_tag="VFJ72_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7480037.1"
                     /translation="ETIPDLSGTRVFMAAGRMDPLIPPDNTQRLAEILKSAGADVDLR
                     WRNTGHGLTYEEVAEAKEWLTKNGL"
     gene            complement(244..669)
                     /locus_tag="VFJ72_11045"
     CDS             complement(244..669)
                     /locus_tag="VFJ72_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7480038.1"
                     /translation="MSAEYFIDTNIFVYQLEALDEDKSATADRIIREGIETKKACISF
                     QVVQECLNTMLRKAEIPLSVEDTRLYMESVLGPLFRVPASMPLYRRALALQARYRYGF
                     YDSLIIAAALDAGCDRLYSEDLQSGQKIERLRIENPFED"
     gene            complement(659..880)
                     /locus_tag="VFJ72_11050"
     CDS             complement(659..880)
                     /locus_tag="VFJ72_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480039.1"
                     /translation="MKNITLSADERLIEDARKRARAEHTTLNEQFRRWLADYTRGEHE
                     AEEAMVVVRELRGKLRVGRKLTRDEMNER"
     gene            1379..1969
                     /locus_tag="VFJ72_11055"
     CDS             1379..1969
                     /locus_tag="VFJ72_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HET7480040.1"
                     /translation="MGKGMKIFDEICSAQREVNTEVLEQTVALAVEIAREGREGRKIG
                     TLFVVGDTEEVLRKSRPMILDPLHGHPDDAKRIDDPNVRETLKELAQLDGAFVVSDEG
                     VVISAARYIDSVSDDLSLPLGLGSRHMAAASVSNRTRAVSVAVSESSMVRVFDDGELI
                     SEIIPELWMWNGYNSRVKGKVSTRQDNEVTVISRAE"
     gene            1959..2537
                     /locus_tag="VFJ72_11060"
     CDS             1959..2537
                     /locus_tag="VFJ72_11060"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HET7480041.1"
                     /translation="MPSEADAVLDFWFGREDEPGYGEFREAWFRKDQAFDDEVRERFG
                     SLYEEAASGALDGWREDARSCLALVILLDQFPRNMFRGDPKTYATDGKAVETARYAVE
                     NALDREVHPFQRHFFYMPFMHSEDLEDQRRSVELFEVLSGGASSTDEPSYAVRHMEIV
                     QRFGRFPHRNEILGRPSTPEEAEFLGQPGSSF"
     gene            2621..>3837
                     /locus_tag="VFJ72_11065"
     CDS             2621..>3837
                     /locus_tag="VFJ72_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480042.1"
                     /translation="MGVIRSRKAVAPCSRGSETWILAATVIGSAMAFIDSTVTNVALP
                     ALQRQLGATAVDAQWIVEAYALFLASLILVGGSLGDHYGRKRIFALGVGVFALSSVGC
                     GISLTPDQLIVARAIQGIGGAMLVPGSLAIISASFDESRRGRAIGTWSGFSGITAALG
                     PVLGGYLVENVSWRAAFLVNVPLAAVVLYIVYRHVPESRDPDARKLDIPGAVLATLGL
                     GGVVFGLIHSQSHGFGNSLVLISLLIGVAALVAFVFVEMRSNEPMMPLSLFRSRNFSG
                     ANLLTLLLYAGLGGSLYFLPFLLIQVHGYSATAAGSSFLPFIVITFLMSRWAGGLITR
                     YGARLPLVIGPIIAAGGFLLYALPGTGGSYWTTFFPAVVVQGLGMALVIAPLTTTAMN
                     SVTGRHSGLASGV"
BASE COUNT          664 a         1197 c         1229 g          747 t
ORIGIN      
        1 ggagacgata ccggacctct ccgggacgcg cgtcttcatg gccgccggtc gcatggaccc
       61 gttgatccca cccgacaaca cccagcgcct cgccgagatc ctgaaaagcg ccggcgccga
      121 tgtggacctc cgctggcgta acaccggcca cggcctcacc tacgaagagg tggcggaagc
      181 gaaggagtgg ctcaccaaga acggtctttg aaaggtcgcc cgttccccac ggcttattcg
      241 agatcagtcc tcgaacgggt tttcgatcct cagacgctcg atcttctgac cactctgcaa
      301 gtcctcgctg tagagccggt cgcaacccgc gtcgagggcc gcggcgatga tgagtgagtc
      361 gtagaaaccg taacggtagc gcgcctggag ggccagagcg cgcctgtaga gcggcatgct
      421 ggcaggaacc cggaacaacg gtcccaaaac actctccata tagagtctcg tatcctcaac
      481 actcagagga atttcagctt tgcggagcat ggtattgaga cattcctgca cgacctgaaa
      541 actgatgcag gctttcttgg tctcgatacc ttcgcggata atacggtccg ccgtcgcaga
      601 tttgtcctca tccagcgctt ccagttggta gacgaatata ttggtgtcga tgaagtactc
      661 agcgctcatt catttcgtcc cgcgtcaact tgcgcccgac ccggagtttg ccccgcaact
      721 ctcgaacaac caccattgcc tcctcggcct catgctcgcc ccgcgtgtaa tccgccagcc
      781 agcgacgaaa ctgctcgttc aaagtcgtgt gttccgcgcg cgccctcttg cgagcgtcct
      841 cgatcaacct ctcatcagca ctcaacgtga tgttcttcat cgaacaaacc ctccgataaa
      901 tcgctaacac tatcgtagtg tacacgagtc tagtgcataa gacgacaaaa cgaagcacgt
      961 ctatcagaat cggcggtctg gctcacttta ggcttccctg gagacctacc cgaacggtca
     1021 ggttgtcagc ggtgctacaa tttttgatga gcgctcatcg ggggccgagg gcacgatatc
     1081 agggctgaaa catacattta acgaacgcat ctgaaaggcg gattattttg gcggagccgg
     1141 tctctttggc ggtgggcggt ccggcgagtg cggcgggcgg cccagcgggt tccccggcga
     1201 cagtccgcgt cggtcggttt caaattcggg agcgggccga ttgcttcggc tgtcgggcct
     1261 gacttgtggg gaatgtgctc ttcgatcaac gccggttgtg ttctggcgga gctggtagag
     1321 gtacggttta tgatgaggtt gctgacggat ttcagagcag agaacggagg ttagggctat
     1381 gggcaaggga atgaagatat tcgatgagat ctgctcggcg caacgcgagg tgaatacgga
     1441 ggtgttggag cagacggtgg cgctggcggt cgagatcgcg cgagagggcc gggaggggcg
     1501 caagatcggt acgctcttcg tcgtgggaga tacggaggag gtgctgcgca agtcgcggcc
     1561 catgatcctg gacccgctcc acggccaccc ggacgacgcg aagcgtatag acgacccgaa
     1621 cgtgcgggag actttgaagg agctggcgca gctcgacgga gcgttcgtgg tctccgacga
     1681 gggcgtcgtg atctcggcgg cccgctacat agactccgta tcggacgacc tgagcctgcc
     1741 cctgggactc ggcagccggc acatggccgc cgcctccgtg tcgaaccgga cgcgggccgt
     1801 ctccgtggcg gtctcggaga gctcgatggt gagggtcttc gacgacgggg agcttatctc
     1861 cgagatcatc ccggagctat ggatgtggaa cggctacaac tccagggtca aggggaaggt
     1921 ctcgacgcgc caggataacg aggtgaccgt gatcagccgt gccgagtgag gccgacgccg
     1981 tcctggactt ctggttcggg cgcgaagacg aaccgggcta cggcgagttc cgggaggcgt
     2041 ggttccgcaa ggaccaggcg ttcgacgatg aggtccgcga acgcttcggg agcctctacg
     2101 aagaggccgc gtccggcgcc ctggacggct ggcgcgagga cgcccggagt tgcctggcgc
     2161 tcgtgatcct cttggaccag ttcccccgca acatgttccg cggagatccg aagacctacg
     2221 ccacggacgg caaggccgtc gagactgcca ggtatgccgt ggagaacgct ctcgaccgcg
     2281 aggtccaccc atttcagcgc cacttcttct acatgccgtt catgcacagc gaggacctag
     2341 aagaccagcg gcgctcggta gagctgttcg aggtgctttc gggaggtgcc agctccacgg
     2401 acgagccctc ctacgccgtg cgtcacatgg agatcgtcca gcgcttcgga aggttcccgc
     2461 accggaacga gatcctcggc cggccgtcca ccccggagga ggcggagttc ctggggcaac
     2521 cgggctcttc cttctgaacg cggccagtgg aggcgcagcc ggcctgcgag atcccgaaag
     2581 gagggacagg gtgtccaaca tagggaatcc gccgtgtgat ttgggagtta ttcgctcccg
     2641 caaggccgtc gcgccatgct cgcggggctc ggagacctgg atactggccg ccaccgtcat
     2701 tggatcggcg atggccttta tagacagcac ggttacgaac gtcgcgctgc ccgcccttca
     2761 gcgccagctc ggcgcgacgg ctgtggacgc gcagtggata gtcgaggcct acgccctgtt
     2821 cctggcgtcc ctgatcctgg tcggcggctc gctcggcgac cattacggcc gcaaacgcat
     2881 cttcgccctc ggcgtcggcg tcttcgccct ctcctccgtg ggatgcggca tctcgttgac
     2941 gccggaccag ctaatcgtgg cgcgcgccat acagggcatc ggcggcgcga tgctcgttcc
     3001 gggatctctg gcgatcatca gcgcctcctt cgacgagtcg cggcgcggca gggccatcgg
     3061 aacctggtcg ggtttcagcg gcataacggc cgccctgggt ccggtcctgg gcggctacct
     3121 ggtggagaac gtctcctggc gggcggcctt cctggtcaac gttccgctcg cggcagtggt
     3181 cctgtacatc gtgtatcgac acgtcccgga gagccgcgac ccggacgcgc gcaagctgga
     3241 tatccccggt gcggttctgg ctaccctggg gctcggaggg gtggtattcg gcctgatcca
     3301 ctctcagagc cacggcttcg gtaactcgct ggtcctgatc tcgctgctga tcggcgtcgc
     3361 ggcgctcgtg gcgttcgttt tcgtggagat gcgctcgaat gagccgatga tgccgctgtc
     3421 gctgttccgc tccaggaact tctccggcgc gaacctgctt acgctcctcc tgtacgcagg
     3481 tctcggcggc tccctctact tcctgccctt cctgctgata caggtccacg gctattcggc
     3541 gacggccgcc ggcagttcgt tcctgccgtt catcgtcatc acgttcctga tgtcgcggtg
     3601 ggccggcggg ctcatcaccc gctacggggc tcgtctgccg ctggtcatag ggccgatcat
     3661 cgccgccgga ggcttcctcc tgtacgcgct gccgggcacc gggggctcct actggacgac
     3721 cttcttcccg gccgtcgtgg tgcagggcct cggcatggcg ctcgtcatag cgcccctcac
     3781 gaccacggcc atgaactccg tcacgggccg ccattccggg ctggcctcgg gcgtcaa
//
LOCUS       DASSMB010000187        18996 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_553826, whole genome shotgun sequence.
ACCESSION   DASSMB010000187 DASSMB010000000
VERSION     DASSMB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 18996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18996
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553826"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VFJ72_11070"
     CDS             complement(<1..1279)
                     /locus_tag="VFJ72_11070"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7480043.1"
                     /translation="MSPYLLGIDSGLTVTKAVVFDEEGRQMGAGSVNNAQKSPRPRWV
                     EQDMDGLWESCCGAIRQALDEAGVKGDEIAGVGVTGHGDGIYLVDEGGRPVRAGVLSL
                     DSRAYEIADRWQESGLADRALELTGQKPFASIAAALLTWFREHEPESFERTRWILYVK
                     DWLRYRLTGEYATDPTEASSGFTDVNTQKYSPEVLSLYGLEDIEEKLPAVVECTGVVG
                     EVTREAAGATGLVAGTPVVAGAHDIDACPVGVGCTKPGQLTMIAGTWSINGVISDRPV
                     LNEAGLCRNFVVPGLWANFGASPASATNLEWFVQNLSPLEIEKAKESGTSPFAFVNDE
                     VKEVLDEESRVFYHPFLYGSPFGDAATAGFFGLRGWHRRGHVLRALFEGTVFNHKWHV
                     DTLRAVSDFSRVRLTGGGARSELWSQMFADALDL"
     gene            complement(1276..2328)
                     /locus_tag="VFJ72_11075"
     CDS             complement(1276..2328)
                     /locus_tag="VFJ72_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HET7480044.1"
                     /translation="MEVLCVGDLFLSSGEFRRAIEKELGEDFGPVREVMWAGERAEDQ
                     HHLQQIMEVDGPEAVPTPDEIVEAVGDAEVIAVHFAPIPKAVLEAGPNLKAVIVARAG
                     TENVNIEAASERGIAVVQLVGRNAPAVAEQAIALMLAETRDIARVDRGIRAGRWPKEF
                     PQEPYDLYGCTVGLIGFGQVARQLAPRISGFDVKLLVYDPYVDAETIEFYGGEKVDDM
                     NRIFRESDFVSLHARLTEETRRFIGREHFEQMKPTAYFINNARSRMVRYDDLYEVLKE
                     GRIAGAALDVHDDEPLGADSPWVELENVTLTPHLAGSTTSTWENSVRMVAEAIHEYAD
                     TGRCENTVNAEALERT"
     gene            complement(2333..3130)
                     /locus_tag="VFJ72_11080"
     CDS             complement(2333..3130)
                     /locus_tag="VFJ72_11080"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480045.1"
                     /translation="MSGAELRLGRLFDRESGRSFIVAFDHGTSLRVPPKVGKPLDVLQ
                     NIVAGEPDGVLISPGMLKQASRLFAFRGAPVPVLRADWTVLDEQMKGDLGEHYRVMCT
                     PQEALALGAGAMVMYLIMGPEEGAMFSDNVRAIADAAQEARRVGIPFIVEATLWGSRM
                     SDKKDPDLLAYCCRIAAELGADAIKTEYTGDPETMAAVIDHCDVPVLTLGGARGGSDE
                     AVVEAARGAIEAGAKGLIFGRNVWQADDPVKMSASLREAVHGAPARR"
     gene            3282..5000
                     /gene="dhaL"
                     /locus_tag="VFJ72_11085"
     CDS             3282..5000
                     /gene="dhaL"
                     /locus_tag="VFJ72_11085"
                     /inference="COORDINATES: protein motif:HMM:NF011049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HET7480046.1"
                     /translation="MRRMLNDATSFKEEMVEGFVAAYRRFVRRIPDASGVMAVGAPKA
                     GKVSVLIGGGSGHYPAFCGVVGQGLADGAVIGDVFTSPSAEQVYRCTKALDGGAGVLL
                     SYGNYAGDVLNFDMAQARCRDEGMDIRTVIVTDDVASAPKGKEHERRGVAGDLYVFKV
                     AGASAARGDDMDGVEQAAQRANANTRSIGIAFAGCTLPGQTEPLFTVEPGMMEVGMGV
                     HGEPGIRTAELLPARGIAELLVDTILEDAPEGAGSRAAVMLDGLGSTKYEELFVLFGD
                     VNRILKERGIEVHEPLVGELVTSLDMAGCSLTLMWLDDELQELHDAPAATPAFSRGSL
                     DLPDEADHAVSVAAGAQVGSESGGDLTESGAGALDALERVLGALRDAEQELGRLDAAA
                     GDGDHGAGMVRGMGAAVEAARAGGGSAGEVLSRAGMAFSGAAGGASGALYGAWISAVG
                     QSLGDDEKPDAAAVHRALKDSLATLEELGKAKPGDKTMIDTLEPFVRSLGEAAKKDAS
                     VAAAWSAALPAAEEGANSTAGMISARGRASKLGERSRGHKDPGAVSMFYVLRAAGEAI
                     SEGGGR"
     gene            5267..6205
                     /locus_tag="VFJ72_11090"
     CDS             5267..6205
                     /locus_tag="VFJ72_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480047.1"
                     /translation="MNFELTMLLAAFAGGLFAAAIGGLPAFVFTGFAVLAGVAAGLGN
                     SDFDVLGNVAFGPVFGPHVSFAGGAAAAAFAYRRGDLENGRDIAAPLAGVNDPLTLLV
                     GGVFGVGGYAFQQLLVGIGIGSYTDSIALTVVVSAIVARLAFGRTGLFGDWRSDPDAE
                     ARGRFVPGGERVWVDYQQGFFQVVVLGIGAGLLSSWLVTAFNAANPDYTPLATVLTFG
                     ISAVSLIFLEFGFSVPVTHHMSLPAGVAATAVITGGGSPLLAMILGLAAGIGGALFGE
                     VYSRLFLIHGDTHIDPPAAAIATMTTIIIVAGLIVG"
     gene            6270..7223
                     /locus_tag="VFJ72_11095"
     CDS             6270..7223
                     /locus_tag="VFJ72_11095"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7480048.1"
                     /translation="MDKDTEGKGIAGHLNLRDIPGASYRDDELGVVHRSGPEDLLAAL
                     SLAKEGRIYDLDSGRWPGMPVFPAHPPFLMTTYRTPQGLKTQKDFDEWRGQNTVNMAL
                     NTEIIIGTVHTGTHLDALSHITCGSDNHWHGGYPMADHLGDFGPLNAEASSIPPFICR
                     GVLVDIPAYKGVEILEAEYLITAEDVQGALEAQGTEVRAGDAVIIRTGYMRAWGVDEE
                     RTEQHFGSGIGHDVAVYLADKGVVLVGGDNESVERNPSPDPENPHPVHIELLIERGIH
                     ILELVHVEDLARDGVYEFLFICLPLRIKGATGSMARPIAIA"
     gene            7309..8856
                     /locus_tag="VFJ72_11100"
     CDS             7309..8856
                     /locus_tag="VFJ72_11100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7480049.1"
                     /translation="MAEGPYFVGIDGGTESVRVGIFDREGTPVVFAGEPYALKHPRPG
                     WAEQDPDEWWSCLAAATRKAMSESGISPDDIAGISSDTTSCTVVAMDDKNRHMRPAII
                     WMDVRAADQARRLGETGDPALKYNGHGPVSAEWMPSKALWLKENEPDTYKNAARICEY
                     QDWMTHKLTGEWTASISNTSIRWYYDRDEGGWPESLYSAVGLDDVLEKFPQKVLNQGE
                     VVGGLSKQAAEEMGLKEGTPVAEGGVDAFVAALGLGVVEPGKMALITGSSHVMIGQAS
                     APIHGQGFFGAYTDAMIPGQYTVEGGQVSTGSIVAWFKNRFAKHAAEEAEKRGVDPYD
                     ILNEMAREVPIGSEGLIVVDYFQGNRTPYTDPLARGMIWGLSLSHGEGHIFRAIVEGI
                     CYGTEHIFRTMRDNDFEPKEIVVAGGPTKSELWMQMHSDVANLPISFTKVGDGAVLGS
                     AMLAAVGAGVYPDIQEAAKNMVHTERTIEPDKDAHDAYGFFVDKYVETYPKMRELMHE
                     TVQHVAG"
     gene            8896..9933
                     /locus_tag="VFJ72_11105"
     CDS             8896..9933
                     /locus_tag="VFJ72_11105"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7480050.1"
                     /translation="MIAAVLKGPNEIEIQEIETPRIGPDEVLVKIGANTVCGTDLRIL
                     RGEKTKGVALPTVLGHELAGHITEVGRDVRGYEAGMQVAVSPGVPCGRCYPCLHDMEN
                     MCTDLHLFGYDIYGGLEEYVRVPARAVELGNLFVAEKELPAEHLALCEPLACCVNGHR
                     RSGTSVDDAVLIMGAGPIGLFHVQLSRLAGARAIIVSEHSEDRRNMARDFGASVTIDP
                     REEDLSEVVKEEAGTLGVDVAVICIGIPALVNEAIQLTRPGGRVNIFAGLAGKGWAEV
                     EANQIHYKELDVTGTSNSRRREFATALRLIESGDVNVEPMVTHRYPLSEAVQAIEMSG
                     SGEGIKVAVMP"
     gene            9963..10907
                     /locus_tag="VFJ72_11110"
     CDS             9963..10907
                     /locus_tag="VFJ72_11110"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET7480051.1"
                     /translation="MATEAKTTEVIPESYSYVFNPYREAVARVKPGERVTIHTNDAFE
                     SRIQSEDDLPGKALATAKFLNPQTGPIYVEGAEPGDTLAVRIESIEPTRDFAVSAMIP
                     YFGGLTSTTMTRTLQEPLPEKVWVWDLIEGGSKLRNDDLGVTLDWDPFMGTLAVAPDL
                     EAISALAPGPFGGNMDVPDVRPGNTVYLPVWNEGALFYTGDCHARQGQGELCGVALEI
                     TSKVTVVFDVIKGKAIEWPRIESEHRIMVVGSARPMEDAARIANTELVLWLEEEHGFD
                     RWDAYQLLTQAGGLYVGNMVDTTYSLVATIEKKYLPGT"
     gene            11094..11903
                     /locus_tag="VFJ72_11115"
     CDS             11094..11903
                     /locus_tag="VFJ72_11115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7480052.1"
                     /translation="MTAIVCLDFDGTLVDEEGSIHPSDVEILANERSVAFVPATGRPL
                     HSVRRTFEQHGLFSGCPIPFPLVLENGAAVYAENEVLRSHRSFEPDLQDVLMRAVLGS
                     ERMSFLLFSLDEVRALRPSETLRALIRRFGLNAQAYDPDEDPAPPLTKVAATAEDMET
                     LRAFAAGTAELPLERTYSLPDVLELTPAGVHKGGGLASLLEGRKVEEVVAVGDGENDL
                     TLFDLATLSFAPESSPPAIRARADRVLDMAEEGLLAPILREITARRRAKHA"
     gene            complement(11943..12698)
                     /locus_tag="VFJ72_11120"
     CDS             complement(11943..12698)
                     /locus_tag="VFJ72_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET7480053.1"
                     /translation="MLKQERQSRILEILRMEGKLVATDLSVALGVSEDTVRRDLRELA
                     EAGTLQRVHGGALPRSSVEVDYAARERQASAGKAAVARAAVKLIEDDQVIILDGGSTV
                     LQVVRRLPADLRATVVTHSPRIAIALAEYPRIEVIVIGGILYKTDLLTVGAGTVQAFS
                     SINADLCFQGIWGLHPEVGITYPNFEEVHVKRAMMKSADQVVALASAEKLGTASSFFV
                     APVSDLTHIVTERTVPEEMLKPYRDLGVSILTG"
     gene            12862..14364
                     /gene="glpK"
                     /locus_tag="VFJ72_11125"
     CDS             12862..14364
                     /gene="glpK"
                     /locus_tag="VFJ72_11125"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET7480054.1"
                     /translation="MEQNYVMGIDEGTTSARTVIFDGDGQIVAEATRELTQHYPRSGW
                     VEHNPVEILDAQLETMRAALEKANLSPQEIRTIGLTNQRETAMVWEKDSGRPIYNAIV
                     WSSRQTAEVIERWAAEGLVEEIREKTGLVSDAYFSASKIAWILENVEGARKRAERGEL
                     LAGTIDAWITWNLTGREKFVTEYSNASRTMMFNIHDLKWDEGLCEAYGIPIEMLPEVL
                     PSDADFGTIGQTFGADIPIQGVLGDQQAGLFGQTCYDVGLAKNTFGTAGVYTMNTGGE
                     PSYRDGLTASVAWGVNGEVTYELEGVVFASGQTIKWLRDSAKLLHGAPDSEWYSGQVP
                     DTQGVYLVPAFTGLCAPYWDMYARAAIIGMSSSTDRRHIIRAGLESMAYQTRDIVETV
                     LSGGELEVPEIRVDGGAVKNSLLCQFQADILGIPVVRPKITEATVLGAAYLAGLTSGL
                     WKDMDALAGLWQEDQVFEPQMSKDRRDELYAGWTAAVELTRGWAKKVNVV"
     gene            14387..15823
                     /locus_tag="VFJ72_11130"
     CDS             14387..15823
                     /locus_tag="VFJ72_11130"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7480055.1"
                     /translation="MRTNSEPINADPTDLAGLQKALNAAEPQGRLAPIGMSRIEIGPE
                     TIDLLPEVVGDLTHSERVVLVSDSTPKWRNGDDLNDLVAGLLGERFAVERAALGEHNP
                     ELHVDDEAMAEAEAAVDGAGCVVVVGSGTVTDICKVATERAGKIPLVVVQSAASVNGF
                     SDDLSVVLKAGVKRTIPSRWPDALLVDLPTLAAAPPEMNAAGFGDAIAMYTGPADWYL
                     ASAVGMDDSYRAAPVKMLLEGGRDLLDGASALRRRDPEALDRLARMLTLDGIAIGVTG
                     TTAALSGTEHLVSHLLDKNAGKRGLPLAFHGAQVGVATVVVAAAWEALLADLDPAAVD
                     VDACFPEPASMEAVVREAFADLDPSGAVGGECWSDDRKKLEKWRANRIRFEEFLADWT
                     RYRAELREMVASPERLCGALAEAGAPARFGELEPAATPETVRWALRNCHLMRNRFTLA
                     DLLFFLGWWDDAFVEKLLERARSVGGGL"
     gene            15872..17461
                     /locus_tag="VFJ72_11135"
     CDS             15872..17461
                     /locus_tag="VFJ72_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET7480056.1"
                     /translation="MTDGRRTVVVTVDAGSGSCRALAWDQSGRMLGLSQQEWDYEPSG
                     HPGGMDFPTESGWRRATECIKAALAQAGVGPDDVKAVTATSMREGFVVYDEEGQEIWA
                     VPNVDARAQKEAEEIIKEGLAEPIYRRGGDWTSIAASARLRWIEANAPEVWERARHMT
                     MLGDWVLQKLSGEYVTDPSLGSSSGLFDLATRDWSRETAAELGISHLLPRVAESGTVV
                     GAVSKKAAEETGLREGTPVVAGGADTQLGLLGGGVVDANTFGVVGGTFWLTAGIADTP
                     LVDPDIRLRTLCHVVPDRWMIEGVGFVHGLSTRYVRDGMLRAANPDFTEENGYKKLDE
                     LAGQCPPGSNGVSYFASNVMNARSWKHAPPSIVGLSPFDIAGTGLGAIFRAVLEEAAY
                     GARGHMEILEEVYGRKVEDVAFVGGPSRSRLWSQILADVLGVRVRVPDVLEATCLGAA
                     LCALVGAGAYSDLAEAAGATVHTASEYEPNRQDKEAYDDLYPRWRDLNDHMIGAADKG
                     LAPHMWTGAGAGSAATSEASA"
     gene            17581..>18996
                     /locus_tag="VFJ72_11140"
     CDS             17581..>18996
                     /locus_tag="VFJ72_11140"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7480057.1"
                     /translation="MAHVGDMIAEMLARYGVERVFGQPGGQTVSLYDGIVKRAPRINH
                     TLTRDERSAAYAADAYARLTGKPGVCDVTVGPGTTKLTDGLMESLNASIPVIALVGEL
                     PRDWAPLRDKGVASQGFDQVTFLQSITKATWMVPSQKALPQLIRSAFRTATTGRPGPV
                     ALVIPHDVMDAEWDESEADVEIDDRYTRAPAYRPVASPDTVREAAELLSRAQRPAIVA
                     GGGVLDSRATDELAQLAETLDAVVVTSFSGKGSVAENTPYSGGVLNPLAGPTANTLVK
                     RADVVVWLGSKVSQNTSVNWTIPTSEQATVHIDVDPIELGRTFRPTVALNGDAKGTLA
                     ALLEEIQPQDHPEWLEEVAQVKAEGQKARDEESGSEGAPLAPQRVMRELDARLAPDDV
                     VISDASFSAGWISAFLPAKEAGRNWIFARGQGGLGYSVPAAIGAASVRPENRVVTISG
                     DGGFYYAIGELATQVQQGQRVI"
BASE COUNT         3244 a         6123 c         6626 g         3003 t
ORIGIN      
        1 cgaggtccag ggcgtccgcg aacatctggc tccacagctc gctccgcgcg ccgccgccgg
       61 tgagccggac ccggctgaag tcggagaccg cccgcagggt atctacgtgc cacttgtggt
      121 tgaagaccgt gccctcgaag agcgcgcgca ggacgtgacc ccgcctgtgc cacccgcgca
      181 ggccgaagaa gcccgccgtc gccgcgtcgc cgaagggcga gccgtagagg aacgggtggt
      241 agaagacccg cgactcctcg tcgagcactt cttttacctc gtcgttcacg aaggcgaagg
      301 gggacgtgcc gctctccttc gccttttcga tctccagggg gctcaggttc tggacgaacc
      361 actccaggtt ggtcgcggag gcgggggagg cgccgaagtt ggcccagagt ccgggaacga
      421 cgaagttacg gcagagaccg gcctcgttca ggacgggtct gtccgagatc acgccgttaa
      481 tggaccaggt gccggcgatc atggtgagct ggcccggctt tgtgcagccg acgccaacgg
      541 gacaggcgtc tatgtcgtgg gcgcccgcta cgacgggcgt gccggcgacg agaccggtcg
      601 ccccggcggc ctcgcgggtg acttcgccga ccacgccggt gcactcgacc acggcgggca
      661 gcttctcctc tatgtcctcc aggccgtaga gggagaggac ctccggggag tatttctgcg
      721 tgttcacgtc ggtgaagccg ctgctcgcct cggtcgggtc ggtcgcgtac tcgccggtca
      781 gccggtagcg gagccagtcc ttgacgtaga ggatccaacg ggtccgctcg aagctctccg
      841 gctcgtgctc cctgaaccag gtgaggagcg cggccgcgat tgaggcgaac ggcttctggc
      901 ccgtaagctc cagcgcccgg tccgcgaggc ccgactcctg ccagcggtcc gcgatctcat
      961 acgcccggga gtcgagcgac agtacgccgg cccggacggg acggccacct tcgtccacca
     1021 ggtagatgcc gtcgccgtgt ccggttacac cgaccccggc gatctcatcg cctttgaccc
     1081 cggcctcgtc caacgcctga cggatagcgc cgcagcagct ctcccagaga ccgtccatgt
     1141 cctgctcgac ccaccgggga cgggggctct tttgggcgtt gttgacggac cccgcgccca
     1201 tctggcggcc ttcctcgtcg aagaccaccg ccttggtgac cgtcagaccg ctgtctattc
     1261 cgagcaggta tgggctcatg tcctctccaa tgcttccgcg ttgaccgtgt tctcgcagcg
     1321 cccggtatcg gcgtactcgt ggatcgcctc ggcgaccatc cgcaccgagt tctcccaggt
     1381 gctcgtggtg ctacccgcga ggtggggggt gagggttacg ttctcaagct ccacccaggg
     1441 gctgtccgcg cccagaggtt cgtcgtcgtg gacgtcgagg gcggccccgg cgatgcggcc
     1501 ctccttcaag acctcgtaga ggtcgtcgta gcggaccatg cggctgcggg cgttgttgat
     1561 gaagtaggcg gtcggcttca tctgctcgaa atgctccctc ccgatgaagc gccgcgtctc
     1621 ttcggtgagg cgggcgtgca ggctgacgaa gtccgactcg cggaagatgc ggttcatgtc
     1681 gtccaccttc tcgccgccgt agaactcgat ggtctcggcg tccacgtacg ggtcgtagac
     1741 caggagcttc acgtcgaaac ccgagatgcg gggcgcgagt tggcgggcta cctggccgaa
     1801 cccgatcagg ccgaccgtgc acccgtagag gtcgtagggc tcctgcggga actccttcgg
     1861 ccacctgccg gcccggatgc cgcggtccac gcgcgcgatg tcgcgggtct cggccagcat
     1921 cagggctatc gcctgctccg cgaccgccgg ggcgttgcgg ccgactagct ggacgaccgc
     1981 gatcccgcgc tcgctcgccg cctcgatgtt tacgttctcg gtcccggcgc gggcgacgat
     2041 gacggccttc aggttcggtc cggcttcgag caccgctttc gggatcgggg cgaagtggac
     2101 cgctataacc tcggcgtcgc ccactgcctc gacaatctca tccggtgttg gcacggcctc
     2161 cgggccgtcc acctccatga tctgctgcag gtggtgctgg tcctcggctc gctccccggc
     2221 ccacatcacc tccctgacgg gcccgaagtc ctcacccagc tccttctcta tggccctcct
     2281 gaactccccc gaggagagga acaggtctcc gacgcacagc acctccatgc ggctacctcc
     2341 tcgcgggcgc gccgtggacc gcttcgcgca gcgaggcgga catcttcacc gggtcgtccg
     2401 cctgccagac gtttcgtccg aagatgaggc ccttcgcgcc ggcctctatc gcgccccggg
     2461 cggcctccac gaccgcctcg tccgagccgc ccctcgcgcc gcccagagtg aggacgggga
     2521 cgtcgcagtg gtcgatcacg gcggccatcg tctcgggatc gccggtgtac tcggtcttga
     2581 tcgcgtccgc ccccagctcc gcggctatgc ggcagcagta cgcgagcagg tccgggtcct
     2641 tcttgtccga catgcgcgag ccccagagcg tagcctcgac gataaacgga atcccgacgc
     2701 gccgcgcctc ctgcgcggcg tcggcaatgg cccggacgtt atcggagaac atcgcacctt
     2761 cctcggggcc catgatcagg tacatcacca tcgccccggc tccgagagcc agggcctcct
     2821 gcggcgtaca catgacccgg tagtgctcgc ccaggtctcc tttcatctgt tcgtccagga
     2881 ctgtccagtc ggcgcgcagg acgggcacgg gagcgccccg gaacgcgaac aggcgggaag
     2941 cctgctttag catgcccggt gagatcagga cgccgtccgg ctctccggcc actatgttct
     3001 gcaaaacgtc caggggcttc ccgaccttcg gtgggaccct gaggctcgtc ccatgatcga
     3061 aggcgactat gaacgaccgc ccgctctccc ggtcgaacag acgccccagc cgcaactcag
     3121 cgccgctcat ctatctctcc tctccgccgc ttccctgctc cgaaccctag cacgccgaca
     3181 gtttcgaggc tacaggagct acgctggctg gctggcgcgg gagatcgcgc gagagtatgg
     3241 tgcccctcgg tggtcggaaa acaaccaggg tgaggagaag catgagacgg atgctgaacg
     3301 atgcgacctc gttcaaggag gagatggtcg aggggtttgt ggcggcgtac agacggttcg
     3361 ttcggcggat acccgacgca tccggtgtga tggccgtcgg cgcgccgaag gcggggaagg
     3421 tatctgtcct gatcgggggc ggttcgggac actatccggc gttctgcggg gtggtcggcc
     3481 agggactcgc ggacggcgcc gtgatcgggg acgtcttcac cagcccctcg gcggagcagg
     3541 tctaccgctg cacgaaggcg ctggacggcg gcgcgggcgt gctcctctcc tacggcaact
     3601 acgcgggcga cgtactgaac ttcgatatgg cgcaggctcg ctgccgggac gaggggatgg
     3661 acatccgcac ggtaatcgtc accgacgacg tggcttcagc tccgaaggga aaggagcatg
     3721 aacggcgcgg cgtggccggc gacctctacg tcttcaaggt ggcgggcgcg agcgcggcgc
     3781 gcggcgacga catggacggt gtggagcagg ccgcccagag agccaacgcc aacacccgca
     3841 gcatcgggat cgccttcgcc ggctgtaccc tgccgggaca gacagagcca ctttttaccg
     3901 ttgagcccgg aatgatggag gtggggatgg gcgtccacgg cgagccgggc atccgcaccg
     3961 ccgagctgct gccggcgcgc gggatcgcgg agctgctcgt cgacaccatc ctcgaagacg
     4021 ccccggaagg cgcggggagc cgcgccgccg tgatgctcga cggtctcggg agcaccaaat
     4081 acgaggagct gttcgtcctc ttcggcgacg tgaaccgtat cctgaaagag agaggtatag
     4141 aggttcacga gccgctggtc ggcgagctgg tcacctcgct ggacatggcg ggctgctccc
     4201 tcaccctgat gtggctggac gacgagcttc aagagttgca cgacgctccg gccgcgacgc
     4261 cggccttctc gcgcggcagt ctcgacctgc ccgatgaagc ggaccacgca gtctccgtcg
     4321 cggcgggggc tcaggtgggc agcgagtcgg ggggagacct gacagagagc ggcgccggag
     4381 cgctcgatgc tctggagcgg gtcctcggcg ccttgcggga cgccgagcag gagctcggcc
     4441 gcctcgacgc ggcggcgggc gatggggatc acggcgcggg catggtccgc ggtatggggg
     4501 cggccgtcga ggccgcgcgg gccggcggcg ggtctgcggg ggaggtcctc tcgcgcgcgg
     4561 gcatggcgtt ctccggcgcg gccggcggcg cctccggcgc actgtatgga gcctggatct
     4621 ccgccgtcgg acagtcgctc ggggacgacg aaaaacccga cgccgccgcg gttcatcgag
     4681 ctttgaaaga ctcgctcgcg accctcgaag agctcggcaa ggcgaagccc ggcgacaaga
     4741 ccatgatcga cacgttggag ccgttcgtcc ggtcactcgg cgaggcggcg aagaaggacg
     4801 cctctgtcgc cgccgcctgg agcgcggcgc tgcccgccgc cgaggaagga gcgaactcga
     4861 cggccggcat gatcagcgct cgcggccggg cctcgaagct cggggagcgg agccgggggc
     4921 acaaggaccc cggcgcggtc tcgatgttct acgtgctgcg ggccgcggga gaggcgatct
     4981 ccgaaggtgg tggccgttag gaaagagcgc ggcttctgtc agaagccgcg ggcaggtctc
     5041 ttcaatggac ccgcgcggat tctctgacag ggctcgcgtc aaacgtgccg gaaagtcgtt
     5101 gaccggaatc ttcggatgca atattctgcc cgacgggcag aggaagccga tcttgccgaa
     5161 cggggaatca tagactggct ggcttccatc gctaagggaa aatgccggga ccactgacgt
     5221 cccggccggt aagggtggcc gagaggcgcc ctgggaggag gtcgtagtga atttcgagtt
     5281 gaccatgctg cttgctgctt ttgccggggg gttgttcgcg gcagcgatcg gcgggctccc
     5341 cgcgttcgtt tttacgggat tcgcggtcct ggccggtgta gcggccggac tcgggaacag
     5401 cgacttcgac gtcctgggca atgtagcttt cggtccggtc ttcggaccgc acgtctcctt
     5461 cgccggaggc gcggcggcgg ctgcgttcgc ctaccggagg ggagacctgg aaaacgggag
     5521 ggacatagca gcgccgctgg ccggcgtcaa cgatcctctt actctgctcg tcggaggggt
     5581 cttcggtgtc ggtggttacg cgttccagca gctcctggtt gggataggga tcggcagtta
     5641 caccgactcc atcgcgctga cggtcgtcgt ctccgccatc gtggcccggc tggccttcgg
     5701 caggacgggg ctcttcggcg actggcgcag cgacccggac gcggaggccc gcggcaggtt
     5761 tgtacccggc ggcgagcggg tctgggtaga ctaccagcag ggcttcttcc aggtcgtcgt
     5821 gctcgggatc ggcgctggtc tgctctcgtc gtggctcgtc acggcgttca acgccgccaa
     5881 cccggactac acgccgctgg cgacggtcct gaccttcggt atctcggcgg tctccctgat
     5941 cttcctggag ttcggcttct cggtcccggt aacacaccac atgtcgctcc ccgccggcgt
     6001 ggcggcgacg gcggtgatca cgggcggcgg cagcccgctc ctcgcgatga tcctcggtct
     6061 ggccgccggc atcgggggcg cgctattcgg cgaggtctac tcgcgcctgt tcctgatcca
     6121 cggcgacacc cacattgacc cgccggccgc cgcgatcgcc acgatgacca ccatcatcat
     6181 cgtagcggga ctgatagtcg gctaggcgga acctccagaa tcgcggcgcc gagcctacgg
     6241 gctttggcgc tgcgggaaag gagcaactga tggataagga taccgaaggc aaggggattg
     6301 cggggcatct caacctgcgc gacatccccg gggcgagcta ccgggatgat gagcttgggg
     6361 tcgtgcatcg ctcaggaccg gaggacctgc tcgcggcgct cagtctggcc aaggagggcc
     6421 ggatctacga cctggactcc ggccgctggc cggggatgcc ggtcttcccg gctcacccgc
     6481 cattcttgat gacgacctac cgcaccccgc agggcctgaa gacccagaag gacttcgacg
     6541 agtggcgcgg gcagaacacg gtcaacatgg cgctgaacac cgagatcatc ataggcaccg
     6601 tccacaccgg gacccacctg gacgccctga gccatatcac ctgcggctcg gacaaccact
     6661 ggcacggcgg atatccgatg gccgaccacc tgggcgactt cgggccgctc aacgccgagg
     6721 cgtcgtccat cccgccgttt atctgccggg gcgtcctggt ggacatccca gcgtacaagg
     6781 gcgtggagat cctggaggcc gagtacctga taaccgccga ggacgtccag ggagcgctgg
     6841 aggcgcaggg gacggaggtc cgggcggggg acgcggtgat cattcgcacc ggctacatgc
     6901 gggcgtgggg cgtggacgag gagcgcacgg agcagcactt cgggtcgggg atcggtcacg
     6961 acgtggcggt gtacctggcg gataagggcg tcgtgctggt cgggggagac aacgagtccg
     7021 tcgagcggaa cccgtcgccg gacccggaga atccgcaccc ggtccacatc gagctgctca
     7081 tagagcgcgg catccacatc ctggagctgg tgcacgtgga ggacctggcg cgcgacgggg
     7141 tctacgagtt cctcttcatc tgcctgccgc tcaggatcaa gggcgccacg ggctcgatgg
     7201 cgcgtccgat cgccatcgcg taagagggcg ccgagggtaa cacctaacag ggaaagagaa
     7261 cgcccttgcc cgttggggct tggggagtcg aaccggaatg gaggagaaat ggccgaggga
     7321 ccgtacttcg tcggcataga cggcggaacc gagagcgtga gggtcgggat cttcgaccgc
     7381 gaggggacgc cggtggtctt cgccggtgaa ccgtacgcgc tgaagcatcc gcgccccggc
     7441 tgggccgagc aggacccgga cgagtggtgg tcgtgcctgg ccgccgcgac caggaaggcg
     7501 atgagcgaga gcgggatctc gcccgacgac atagcgggca tctcgtcgga taccacgagc
     7561 tgcaccgtcg tcgccatgga cgacaagaac cgccacatgc gaccggcgat catctggatg
     7621 gacgtgcggg ccgccgacca ggccaggaga ctcggcgaga ccggcgaccc ggcgctgaag
     7681 tacaacggcc acggtcccgt ctccgccgag tggatgccct cgaaggcgct ctggctcaag
     7741 gagaacgagc ccgacaccta caagaacgcg gcgcgcatct gcgagtacca ggactggatg
     7801 acccacaagc tcaccggcga gtggacggcg tcgatctcca atacctcgat ccgctggtac
     7861 tacgaccggg acgagggcgg atggcccgag agcctctact cggcggttgg gctggacgac
     7921 gtgctggaga agttccccca gaaggtgctg aatcagggcg aggtcgtcgg gggcctgagc
     7981 aagcaggccg cggaggagat gggcctcaag gaagggacgc ccgtcgccga gggcggcgtg
     8041 gacgcgtttg ttgcggcgct ggggctcggg gtggtggagc ccggaaagat ggcgctcatc
     8101 accggctcgt cgcacgtcat gatcgggcag gcgtcggcgc ccatccacgg ccaggggttc
     8161 ttcggggcgt acacggacgc catgatcccc ggacagtaca ccgtggaggg cggtcaggtc
     8221 tcgaccggct ccatagtcgc ctggttcaag aaccgcttcg ccaaacacgc ggcagaggag
     8281 gccgagaagc gcggcgtgga cccgtacgac atcctcaacg agatggcgag agaggtgccc
     8341 ataggctccg aaggtctgat cgtggtggac tacttccagg gcaaccggac gccgtacacc
     8401 gatccgctgg cccgaggcat gatctggggt ctctccctct cccacggcga gggtcacatc
     8461 ttccgggcca tcgtcgaggg tatctgctac ggcacggagc acatcttccg caccatgcgc
     8521 gataacgact tcgagccgaa ggagatcgtg gtcgccggag gcccgaccaa gagcgagctg
     8581 tggatgcaga tgcactcgga cgtggcgaac ctgccgatct ccttcacgaa ggtcggcgac
     8641 ggcgcggtgt tgggctcggc tatgctggcc gccgtcggag ccggagtcta cccggacatc
     8701 caggaagccg cgaagaacat ggtccacacc gagcgcacca tcgagccgga caaggacgcc
     8761 cacgacgcat acgggttctt cgtggacaag tacgtcgaga cctatccgaa gatgcgcgag
     8821 ctgatgcacg agacggtgca gcacgtcgcc ggatgagggg gatccattga ggagcgcgag
     8881 aaacggcggc ggcccgtgat cgccgccgtc ttgaaagggc cgaacgagat cgagatccag
     8941 gagatagaga ctcccaggat cggtccggac gaggtcctgg tcaagatcgg ggcgaacacg
     9001 gtctgcggca cggacctgcg catcctgcgc ggggagaaaa cgaagggtgt ggccctcccg
     9061 accgttctgg gccacgagct ggcgggtcac atcaccgagg tcggccggga cgtgcggggc
     9121 tacgaggccg ggatgcaggt ggcggtctcg ccgggcgtgc cgtgcggcag gtgctacccg
     9181 tgcctgcacg acatggagaa catgtgcacc gatctgcacc tgttcggcta cgacatctac
     9241 ggcggcctgg aagagtacgt ccgggtgccc gccagggcgg tcgagctcgg caacctcttc
     9301 gtcgcggaga aggagttgcc cgccgagcac ctggcgctct gcgaaccgct agcctgctgc
     9361 gtgaacggcc accgccgctc cggcacgagc gtggacgacg cggtactcat catgggcgcg
     9421 gggccgatcg ggctcttcca cgtccagctc tcccggctcg ccggagcgag ggccatcatc
     9481 gtcagcgagc actccgagga ccggcggaac atggcgcggg acttcggggc gagcgtcact
     9541 atagacccca gggaagagga cctctccgag gtggtgaagg aggaggccgg gacgctcggc
     9601 gtggacgtgg ccgtgatctg catcggtatc ccggcgctgg tcaacgaggc gatacagctc
     9661 acccggcccg gcgggcgggt caacatcttc gccgggctgg ccggaaaggg ctgggcggag
     9721 gttgaggcca accagatcca ctacaaggaa ctggacgtta ccgggacctc gaactcccgg
     9781 cgccgagagt tcgccacggc cctgcgcctc atcgagagcg gcgacgtaaa cgtggagccg
     9841 atggtgaccc accggtaccc gctctcagag gcagtacagg cgatagagat gtccgggagc
     9901 ggcgaaggca tcaaggtagc ggtaatgccc tagcgggcga aatctgaaca gaggaggttc
     9961 ttatggcgac tgaagccaag actaccgagg taatccccga gtcctacagc tacgtcttca
    10021 acccctaccg ggaggcggtc gcgcgggtga agcccggcga gcgggtgacg atccacacca
    10081 acgacgcgtt cgagagccgt atacagagcg aggacgacct gccggggaag gccctggcga
    10141 cggcgaagtt cctcaacccg cagaccggcc ccatctacgt cgagggcgcg gagcccggcg
    10201 acacgctggc ggttcgcatc gagagcatcg agccgacgcg agacttcgcc gtgagcgcca
    10261 tgatcccgta cttcggcggc ctcacctcga ctaccatgac ccgcacgctc caagagccgc
    10321 tgccggagaa ggtctgggtc tgggatctga tcgaaggcgg cagcaagctg aggaacgacg
    10381 acctcggcgt gacactggac tgggacccgt tcatgggcac gctcgccgtc gccccggacc
    10441 tcgaagccat ctccgcgctg gcgccgggac cgttcggcgg caacatggac gtgccggacg
    10501 tgcgtccggg aaacacggtg tacctgccgg tctggaacga gggcgcgctg ttctacacgg
    10561 gtgattgtca tgcgcggcag ggtcagggcg aactgtgcgg ggtggcgctg gagatcacct
    10621 cgaaggtgac ggtggtcttc gacgtgatca agggcaaggc catcgagtgg ccccgcatag
    10681 agtcggagca caggatcatg gtcgtcggca gcgcccgccc gatggaggac gccgcccgca
    10741 tcgccaacac ggaactcgtc ctatggctgg aagaggagca cggcttcgac cgctgggacg
    10801 cctaccagct actcacccag gccggcggcc tctacgtggg caacatggtg gacacgacct
    10861 actcgctcgt ggcgaccata gagaagaagt acctgccggg aacctgacgc acgcggcgat
    10921 agcgcgctcg atcctctagc gcaccctgat actagcgtac cttcccgtgc gctgacagac
    10981 gcccgcgtgc tagaaagccg ccgagccgca aggacgagag atgcgtctgt gacgaaaacc
    11041 tgtgaagttc agaggatcat ctacggatcg cgtgtctgta ctggaggtgc gggatgaccg
    11101 cgatcgtctg tctcgacttc gatgggacgc tggtggacga ggagggcagc atccacccgt
    11161 ccgacgtcga gatactggcg aacgagcgct ccgtcgcctt cgtgccggcc acgggccgtc
    11221 ccctgcactc ggtgcggcgg actttcgagc agcacgggtt gttctccggg tgtcccatcc
    11281 cgttcccgct cgtcctggag aacggagcgg ccgtatacgc ggagaacgag gtactgcggt
    11341 cgcaccgttc cttcgagccg gatctccagg acgtgctgat gcgcgccgtg cttggctcgg
    11401 agcggatgag cttcctgttg ttcagcctgg atgaggtgcg ggcgttgcgg cccagcgaga
    11461 cgctgcgggc cttgatccgg cgcttcggcc tgaacgcgca ggcgtacgac ccggacgaag
    11521 acccggcgcc accccttacg aaagtggccg ccaccgccga ggatatggag acgctgcgcg
    11581 ctttcgcggc cggtacggcg gagcttccgc tggagcgcac ctattccctg ccggacgtgc
    11641 tggagcttac gccggccggc gtacacaagg gtggtggtct ggcctccttg ctggagggca
    11701 ggaaggtaga ggaggtcgtg gccgtgggcg acggcgagaa tgatctgacg ttgttcgacc
    11761 tggctacgct gtccttcgcc ccggagagca gcccgcccgc catccgggcc cgcgcggatc
    11821 gcgtcctcga catggcagag gaggggcttc tcgccccgat cctccgggag atcaccgcgc
    11881 ggcggcgggc gaagcacgca taaacgcgcg ttccgaactc cgccagtacc tgcggagagc
    11941 cttcagcccg tcaggatgct gacgccgaga tcccggtatg gtttgagcat ctcctccggg
    12001 acggtgcgtt cggtgactat gtgggtgaga tcgctgaccg gcgcgacgaa gaatgatgac
    12061 gccgtgccca gcttctccgc cgaggccagg gccactacct ggtccgcgct cttcatcatc
    12121 gctcgtttga cgtgtacctc ctcgaagttg ggatacgtta tcccgacctc ggggtgcagg
    12181 ccccatatcc cctgaaagca caggtcggcg ttgatcgagg agaacgcctg gacggtgccg
    12241 gcgcccacgg tcagcaggtc cgtcttgtag aggatgccgc cgatcacgat cacctcgatc
    12301 ctgggatact cggcgagcgc tatggctatg cgcggactgt gcgtcaccac cgtggctctt
    12361 agatccgcgg gcagacgccg gaccacctgg agcactgtgg atccaccgtc caagattatt
    12421 acctggtcgt cctcgatcag ctttaccgcc gcccgcgcga ccgccgcctt acccgcagac
    12481 gcctgccgct cacgggccgc gtaatccacc tccactgacg agcgcggcag cgccccgcca
    12541 tgcacccgct ggagagtccc ggcctccgcc agctcgcgca ggtcccgccg aaccgtatcc
    12601 tccgagacgc ccagcgcaac gctcagatcc gtcgccacga gcttcccctc catccgcaat
    12661 atctccagga ttcgactctg gcgttcttgc ttcagcatca cggtccttcc tgccgattta
    12721 tgcgcgtatt ttccgattaa tacatataaa tcgttgactt tgcgtttttg tgcgtgtatt
    12781 gtctcgcgca agggatttta ctcggctctc tgggggtgtc tagcgggacc gggtagagcg
    12841 gaatgggaaa ggggatttac tgtggagcag aattacgtga tgggcatcga cgagggcacc
    12901 acgagtgcgc ggacggtgat ctttgacggc gacgggcaga tcgtcgccga ggctacgcgc
    12961 gagttgacgc agcactatcc gaggtccggt tgggtcgagc acaacccggt ggagatactc
    13021 gacgcccagc tcgagaccat gcgcgccgcg ctggagaaag caaacctctc tccccaggag
    13081 atacgcacta tcgggctcac gaaccagcgg gagaccgcga tggtatggga gaaagactcc
    13141 ggaaggccca tctacaacgc catcgtctgg tcctcgcggc agacggccga ggtgatcgaa
    13201 cgctgggcgg ccgagggtct ggtcgaggag atccgggaga agaccgggct ggtcagcgac
    13261 gcatatttct cggcgtcgaa gatcgcctgg atcctggaga acgtcgaggg cgccaggaag
    13321 agggcggaga ggggagagct tctggcgggg accatagacg cctggatcac ctggaacctc
    13381 accggccggg agaagttcgt caccgagtac tcgaacgcct cccggacgat gatgttcaac
    13441 atccacgact tgaagtggga tgaggggctg tgcgaagcgt acggcatccc gatcgagatg
    13501 ctgccggagg tcctgccctc cgacgcggat ttcgggacta tcggccagac cttcggggcg
    13561 gacataccga tacagggcgt gctcggcgac cagcaggccg ggctcttcgg ccagacctgc
    13621 tacgacgtgg ggctcgccaa gaacaccttc gggaccgcgg gcgtctacac gatgaacacc
    13681 ggcggcgagc cttcctacag ggacgggctt accgccagcg tggcgtgggg cgtcaacggc
    13741 gaggtgacct acgagctgga gggggtcgtc ttcgcgtccg ggcagaccat caagtggctg
    13801 cgcgactccg cgaagctgct ccacggcgcc ccggacagcg agtggtacag cggccaggtg
    13861 ccggatacgc agggcgtgta cctcgtgccg gctttcacgg ggttgtgcgc tccgtactgg
    13921 gacatgtacg ctcgcgcggc catcatcggg atgtcgagca gcaccgaccg ccggcacatc
    13981 atacgggcgg gactggagtc gatggcctac cagaccagag acatcgtcga gacggtcctg
    14041 tccgggggag agctggaggt gccggagatc cgcgttgacg gcggggcggt aaagaactcc
    14101 ctgctctgcc agttccaggc ggacatcctc ggcataccgg tcgtgcggcc caagatcacg
    14161 gaggccaccg tcctcggcgc ggcctacctt gcggggctta ccagcgggct ctggaaagac
    14221 atggacgcgc ttgctgggct gtggcaggaa gatcaggtct tcgagccgca gatgtccaag
    14281 gaccgcagag acgagctgta cgcgggctgg acagccgccg tggagctcac ccgcggctgg
    14341 gccaagaagg tcaacgtcgt ctgatcctct gaccgagcct ctctatatgc gaaccaacag
    14401 cgagccgata aacgcggacc cgacggacct tgccggtctg caaaaagcgc tgaacgccgc
    14461 cgaaccacag ggccggctgg cgcccatcgg catgagccgg atcgagatcg gtcccgagac
    14521 aatcgacctt ctacccgagg tcgtcgggga cctcacccat agcgagcggg tcgtgctcgt
    14581 gagcgactcg actcccaaat ggcgaaacgg cgatgacctc aacgacctgg tggcgggctt
    14641 gctcggcgag cgtttcgcgg tcgagcgggc cgcgctcggc gagcataatc cggagctgca
    14701 cgtggatgac gaggctatgg cggaggcgga agcggcggtg gacggagccg ggtgcgtggt
    14761 ggtggtcggc tccggcaccg ttacggacat ctgcaaggtg gctaccgaac gggcgggcaa
    14821 aattccgcta gtcgtcgtgc agagcgccgc ctcggtcaac ggcttctccg acgacctgtc
    14881 ggtggtgctg aaggccgggg tgaagcggac catcccatcg cgatggccgg acgccctcct
    14941 tgtagacctt ccgacgctgg ccgccgcgcc gcccgaaatg aacgcggccg gctttggtga
    15001 cgccatcgcc atgtacactg gccccgccga ctggtatctg gcctctgccg tggggatgga
    15061 cgactcgtac cgcgctgcgc cggtgaagat gctgctcgaa ggggggcgcg atctcctcga
    15121 cggcgcctcc gcgcttcgcc gccgcgaccc cgaagccctc gaccggctcg cccggatgct
    15181 caccctcgat ggaatcgcca tcggcgtgac cgggacgacg gccgccctct cggggacgga
    15241 gcacctggtg agccacctgc tcgacaagaa cgcaggcaaa cgcggcttac cgctcgcttt
    15301 tcacggcgcc caggttggcg tagccacggt ggtcgtcgcc gcggcgtggg aggcgctgct
    15361 cgcggacctg gacccagcgg cggtagacgt ggacgcctgt ttcccggagc cggcgtcgat
    15421 ggaggccgtg gtgcgagaag ctttcgcaga tctggacccc tcgggagcgg tcggcggcga
    15481 gtgctggagt gacgaccgca agaagctgga gaagtggcgg gcgaaccgca taaggttcga
    15541 agagttcctc gcggactgga cccggtaccg ggccgagctg cgggagatgg tcgcctcgcc
    15601 ggagcgacta tgcggggcgc tcgcggaggc cggcgctccc gcgcgtttcg gggagttgga
    15661 gccagccgcc accccggaga ccgtgcgctg ggcgctgcgg aactgccacc tcatgcgcaa
    15721 ccgtttcacc ctggcggacc tgctcttctt ccttggctgg tgggacgacg ccttcgtcga
    15781 aaagctcctc gaacgcgccc gctcggtagg aggcggactt tagatatgag accgaaaaac
    15841 ataggtgtaa aggtagggaa gggagacaag aatgactgat ggtagaagga ccgtggtcgt
    15901 gacggtggat gcgggatcgg ggagttgccg ggcgctggcg tgggaccaga gcgggcgcat
    15961 gctcgggctc tcgcagcagg agtgggacta cgagccctcg gggcatccgg gcggcatgga
    16021 cttccccacc gagagcggct ggcggcgggc gacggagtgc atcaaggccg cgctcgccca
    16081 ggccggggtg gggccggacg acgtcaaggc ggttacggcg accagcatgc gcgagggttt
    16141 cgtcgtctac gacgaggagg ggcaggagat ctgggccgtc ccgaacgttg acgcccgcgc
    16201 ccagaaggag gccgaggaga tcataaagga gggtctcgcg gagcccatct accgccgggg
    16261 cggggactgg acctccatcg ccgcctccgc ccggctgcgc tggatcgagg cgaacgcgcc
    16321 ggaggtgtgg gagagggcgc gccacatgac gatgctcggc gactgggtcc tccagaagct
    16381 ctcgggcgag tatgtcaccg acccatccct cggctcctcc tcggggctct tcgacctggc
    16441 tacccgcgac tggtcgcggg agacggcggc agagctcggc atctcgcacc tcctgcccag
    16501 ggtcgccgaa tcgggaactg ttgtcggcgc ggtcagcaag aaggccgcgg aggagacggg
    16561 actcagggag ggcacgccgg tggtggcggg cggcgccgac acccagctcg ggctcctcgg
    16621 gggcggggtt gtggatgcca acaccttcgg cgtggtgggg gggacgttct ggctgacggc
    16681 cgggatcgcc gacaccccgc tggtagaccc ggatatccgg ctgaggacac tgtgccacgt
    16741 ggtcccggac cgctggatga tcgagggcgt gggcttcgtg cacgggctct ccacccgcta
    16801 cgtgcgcgac gggatgctgc gggccgcgaa cccggacttc accgaggaga acgggtacaa
    16861 gaagctcgac gagcttgccg ggcagtgccc ccccggctcc aacggcgtct cctacttcgc
    16921 ctcgaacgtc atgaacgcca ggtcgtggaa gcacgcgccg ccgtccatag tcggtctcag
    16981 cccctttgat atcgccggga ccgggctcgg ggccatcttc cgcgccgtac tggaggaggc
    17041 cgcctacggg gcgcgcgggc acatggagat cctcgaagag gtctacgggc gcaaggtgga
    17101 ggacgtcgcc ttcgtgggcg gcccgtcgcg cagccggctc tggtcccaga tcctggcgga
    17161 cgtgctgggc gtgcgggtgc gggtgccgga cgtcctggag gctacctgcc tcggcgcggc
    17221 gctgtgcgcc ctcgtcggcg ccggcgcgta ctccgatctt gccgaagcgg cgggcgccac
    17281 ggtccacacc gccagcgagt acgagccgaa ccggcaagac aaagaggcct acgacgacct
    17341 ctacccgcgc tggcgcgacc tcaacgacca tatgatcggc gccgccgaca agggactcgc
    17401 gcctcacatg tggacgggcg cgggcgccgg gagcgcggcc actagcgagg cttccgcgta
    17461 gagatgggta gagagtagta ccgtgggcgc gagaatagta gcagcccgga gtcagaggcg
    17521 gcggtcgtcg ggatcgccgc cttataagag aaggttcatc accataagga ggaagtcgag
    17581 atggcccacg ttggagatat gatcgcggag atgctcgccc ggtacggggt ggagcgggtc
    17641 ttcgggcaac cggggggaca gaccgtctcc ctctacgacg gcatagtgaa gcgggcgccg
    17701 cgcataaacc acacgctcac gcgggacgaa cgcagcgccg cctacgccgc cgacgcctac
    17761 gcgcggctca ccggcaagcc gggcgtctgc gacgtgaccg tgggacccgg caccaccaag
    17821 ctgaccgacg gcctgatgga gagcctgaac gcctcgatcc cggtgatcgc gctggttggc
    17881 gagctgcccc gagactgggc gcctctgcgc gacaagggcg tagcctcgca gggcttcgat
    17941 caggtcacct tcctccagag catcacgaag gcgacgtgga tggtcccgag ccagaaggcg
    18001 ctgccgcaac tgatccggtc ggccttccgc acggccacca cgggccgtcc cggtcccgtc
    18061 gccctcgtta tcccgcacga cgtcatggac gccgagtggg acgagtccga ggccgacgtg
    18121 gagatagacg accgctacac cagggcgccg gcctaccgcc ccgtcgcctc gccggacacg
    18181 gtaagggagg cggccgagtt gctctcccgc gcgcagcggc cggccatcgt cgccggaggc
    18241 ggagtcctcg actctcgcgc caccgatgag ctcgcgcaac tggcggagac tctggacgcc
    18301 gtggtcgtga ccagcttctc gggcaagggc tccgtcgcgg agaacacgcc ctactccggc
    18361 ggggtactga acccgctcgc cggcccgacc gcgaacacgc tcgttaagcg ggccgacgta
    18421 gtagtctggc tcggctccaa ggtcagccag aacaccagcg tcaactggac gatacccact
    18481 tccgagcagg ccaccgtaca catagacgtg gacccgatag aactcggccg caccttccgc
    18541 ccgaccgtcg ccctgaacgg tgacgcgaag ggcacactgg cggccctgct cgaagagata
    18601 cagccccagg accatcccga gtggctggag gaggtggcgc aggtcaaggc cgaggggcag
    18661 aaggcgcgcg atgaggagtc cggctccgag ggagcgccgc tcgccccgca gcgcgtgatg
    18721 cgggagctcg atgccaggct cgccccggac gacgtggtca tcagcgacgc gagcttctct
    18781 gctggttgga tctccgcctt ccttcccgct aaagaggcgg ggcggaactg gatcttcgcc
    18841 cgcgggcagg gcggtctcgg ttactcggtc cccgcggcca tcggcgccgc cagcgtgcgc
    18901 ccggagaacc gcgtcgtcac cataagcggc gacggcggct tctactacgc tatcggggag
    18961 ctcgccaccc aggtgcagca gggccagcgc gtcata
//
LOCUS       DASSMB010000188        13437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_554191, whole genome shotgun sequence.
ACCESSION   DASSMB010000188 DASSMB010000000
VERSION     DASSMB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13437
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554191"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFJ72_11145"
     CDS             complement(<1..282)
                     /locus_tag="VFJ72_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480058.1"
                     /translation="MAALSFGLFVASIPPYFDELRSVCTRGARACADNGLLTNARLRE
                     LRSLGLSASFYAAYNTALGIIFELVWCAIGVVIFLRRSDDRMALLAATML"
     gene            complement(381..1484)
                     /locus_tag="VFJ72_11150"
     CDS             complement(381..1484)
                     /locus_tag="VFJ72_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480059.1"
                     /translation="MNTDPYWGAEAVIALVFPAVGAVIVSRYPVNALGWIFCALGVSA
                     AASGFVSQYATHALLSDPGSQLGIEAVWFSSLLGNLSFVSLVFVPLLFPNGRPPSRRW
                     RPVLWLIAVVITLTVVSFAFTPGPLAEYPSAKNPFGIEGLQPVFDFLSVAAGPVGIFC
                     MLASVTSLVVRFWHSRGDEREQLKWFTYAIVLGLSSLVLNGIFPDLAWLIGGLGTAAL
                     PVAIGIAVVKYRLYDIDVIINRTLVYAALTVSLVLVYFGGVVALQYVFRAFTGDKSQL
                     AIVASTLAIAALFSPLRRRVQDLVDRRFYRRKYDAARTLEAFGARLRDETDLESLSGY
                     LVDVVRETMQPSHVSLWLRSPAEQESKGVGEGA"
     gene            complement(1577..2860)
                     /locus_tag="VFJ72_11155"
     CDS             complement(1577..2860)
                     /locus_tag="VFJ72_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480060.1"
                     /translation="MRRRMTFWLAWSLGGLSVAMFVAGVTLALLAWNVAVPAARVPSG
                     GFGDLLIFAPFLAFPIVGALIASKRPENPIGWICLTVGLFWMLIVAGEGSTAYSLATT
                     GSVTGPVMLDALTQSIWVPPVGLLGTFMVMLFPDGRLPSRRWRPLAWFSGAVILLASV
                     ALDIGPGPLPNRGGISNPLGIEGYPWLQYAGVALVLLLPVCILASAFSLISRYRHSDK
                     EVRQQIKWLAFAASFIGANYLVLLVSEIFFASDALLSDGTTPLWASLEQHLLLVSFAC
                     IPTAIGFAVLKHRLYDIDIIINRALVYGSLTATLALVYVGSVVALQYVFRTFTGSESQ
                     LAIVVSTLSIAALFGPLRRRIQDVIDRRFYRRKYDAARTLEAFGKTLRDETELEILSN
                     DLVSVVRETLQPEYASLWLRPPDEAKPRMKAEQGP"
     gene            complement(2857..4098)
                     /locus_tag="VFJ72_11160"
     CDS             complement(2857..4098)
                     /locus_tag="VFJ72_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480061.1"
                     /translation="MRRRWAALLWTAVALQVVLVLLGILFEALDRPASSLGSGFAVDA
                     VVGAASILAFPAVGALIFWRRPEHPIGWLFCVVNVGWAINHFAGPYAKYALAVNPGAV
                     PGGKLAAWFYFWPGPVSVGLLILLFLLFPDGRLLSPRWRTVAWVTVGYVVVGATASAF
                     APGRIDDTIGFKVDNPAGIGGPIGHLLRPLAGIVQPLPVLLLVAALISLALRQRRSRG
                     QERQQLKWFTSSLILYVVLVGADTVSFFYYGSESAMPGWASLFNDIAISSYGLIPIAA
                     GIAILRYRLYEIDLLINRALVYSALTVSLAAVYVGSVVLLQMVLRSLTGESSQLAVVA
                     STLAIAALFNPLRRRVQALVDRRFYRRKYDAARTLEAFGSRLRDETDLDDLSDHLVAV
                     VRETMQPEHVSLWLRTRDGKR"
     gene            complement(4329..5756)
                     /locus_tag="VFJ72_11165"
     CDS             complement(4329..5756)
                     /locus_tag="VFJ72_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7480062.1"
                     /translation="MTERGVYDAVVVGSGPNGLAAAVELARSGRSVAVLEAGETIGGA
                     VRSAELTLPGFVHDLGSAIHPLGFASPFFKSLPLEEHGLEWVHPPASLAHPFDNGTAA
                     VLERSVAATAERLGPDANAYRKLMQPLAEDGPRLASLLSGTLNVPRHPFALAANGLRA
                     LGSARALAESLFEGEKARGLFAGNAAHSFLPLDQRPSAMFGLVMGTLGHAFGWPFPKG
                     GSQKLADALASYLRSLGGEVYTGVRVKSVEEVPRTRAVLFDVAPRNLLDIAGEHFTER
                     YRNALKRYRYGPGVFKVDFALDGPVPWKADGCSRAGTVHLGGALDQVSAGEAAVGRGE
                     HPERPFVLLAQQSLFDPTRAPEGKHAVWAYCHVPNGSTLDMTGRIEDQIERFAPGFKE
                     CILAKTAMSTSDLERWNANLVGGDINGGYMDLRQLFTRPTPRLNPYSTPARGLYICSS
                     STPPGGGVHGMCGYLAARAALRYLK"
     gene            complement(5782..6552)
                     /locus_tag="VFJ72_11170"
     CDS             complement(5782..6552)
                     /locus_tag="VFJ72_11170"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HET7480063.1"
                     /translation="MTTPTPPPEKKDPREPRDAAFWAKYTEKLEVSEVPEGASNVNVH
                     GRREVGALQGFGKMWQKTYRIRLTGVEATPAEVVRIWKEKFPEFQPSNSRFYPSLAGV
                     APGEVLFISASVGGMPVHTGVRVIYADDESFTVMTPEGHPESGWNTFSAYEDEDGVTV
                     AQIQSLARANDPIYELGFRVVGSTEQERIWTHVLKSLAAHFGVNEPVDLEKVCVDPKV
                     QWSQAMNVWHNAGARSMLYTMAAPTRWMRGLMRRQNPR"
     gene            complement(6557..7288)
                     /locus_tag="VFJ72_11175"
     CDS             complement(6557..7288)
                     /locus_tag="VFJ72_11175"
                     /inference="COORDINATES: protein motif:HMM:NF020906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 family protein"
                     /protein_id="HET7480064.1"
                     /translation="MTAQGQPRDAAYWAEYTETLKVSEVPEGAVNLNVDGRRTVGPLQ
                     GFGKMWQKTYRVRLEGADTTPAGVVKTWKEDFPKFWMPGNRFLTSRAGLAPGEVALIN
                     SDLPGSRWFSTGIVVIYADDTSFTYMTPEGHPFSGWITFSSHIAEDGTTVAQVQLLIR
                     ANDPLYELMMPVALHRVEDMTWQHTLRSVAAHFGAPGEVETRVVCVDPKRQWRHYKNI
                     RHNAAIRSALHAMTAPLRRNNQGKE"
     gene            complement(7316..7675)
                     /locus_tag="VFJ72_11180"
     CDS             complement(7316..7675)
                     /locus_tag="VFJ72_11180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7480065.1"
                     /translation="MPQAQVKMNVRKAGRKAAIIDIEGELAAFAEGVLMDAYNQASAD
                     GARVIILNFEGLEYMNSSGIGLLVTLLIRINREKQRLLTYGLSEHYRSIFQITRLDDA
                     IAIYDAEDEAVKAADGM"
     gene            complement(7705..8064)
                     /locus_tag="VFJ72_11185"
     CDS             complement(7705..8064)
                     /locus_tag="VFJ72_11185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET7480066.1"
                     /translation="MMAARHLDVEVRQEPGAIVLDLRGEINGLAMEALNEAYAQAEAE
                     DPEVILLNFAQVDYINSTGIALIVGLLGRARASGRRLLACGLSEHYVEIFNITRLSDF
                     MNVFPDEDSALTETPVS"
     gene            complement(8061..8549)
                     /locus_tag="VFJ72_11190"
     CDS             complement(8061..8549)
                     /locus_tag="VFJ72_11190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7480067.1"
                     /translation="MTAPKQSGKTERNGARRVLAELSVPSEPGNERRAMEEVAGAVRE
                     LGLPPPTLDRLKTAVAEATMNAMEHGNQYRADVPVLIEVSATEADLSVRITDEGSGPP
                     PEPDAETPDIEAKLEGMQTPRGWGLFLIKNMVDEMRVSGDENHHTIELIVRLDGRAKG
                     GK"
     gene            complement(8546..9715)
                     /locus_tag="VFJ72_11195"
     CDS             complement(8546..9715)
                     /locus_tag="VFJ72_11195"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HET7480068.1"
                     /translation="MTTQQKKGRTWRTMFRREDEAPVTTLTEAPEEFAVVEAPEIEIA
                     PNDPILAYLASVSGVVEVERLDLDSPALRAMKAADVKMLVPLVSQGELIGLLNLGPRL
                     SQQEYSADDRKLLNDLATQTAPAVRVAQLVRQQQLEAQERERIEQELRVARLIQQTLL
                     PKSVPEIEGYQVEAYYQPAREVGGDFYDFLELEDGNLGLVVGDVTDKGVPAALVMATT
                     RTMLRAAAQRLDTPGEVLRRVNDVLVPDIPPNMFVTCLYAILDPGTGQLRYANAGHDL
                     PYRRRADRSGGAEELRATGMPLGLMPGMGYEEKEIVLERGEGVLFYSDGLVEAHDPQR
                     EMFGFPRLQGLVGAHRSGGSPLVSFLLAELARFTGQGWEQEDDITLVTLERSEAS"
     gene            complement(10117..11292)
                     /locus_tag="VFJ72_11200"
     CDS             complement(10117..11292)
                     /locus_tag="VFJ72_11200"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET7480069.1"
                     /translation="MGRRAIVVVLDGLGAGNAPDAADFGDEGANTLANTARAVGGLDA
                     PNLQALGLGNVIEIEGVPHVKSPGASHGLMVEKAAAKATLAGHWEMMGIVLDDPLPTY
                     PDGFPEEILLRFEDETEREVIGNRPASGTEIIEELGPDQEKTGSWIVYTSADSVFQIA
                     AHTGVIPLEELYEACEKAHKMLIGEGQILVERVIARPFHGEAGAYERENENRHDYGIT
                     PPSETYMDRIKNAGHEVVAVGKIRDIFDGQGITEHLPAPPDDSAKVDAILDALGRVES
                     GLIFANLVDFDAKYGHRRDPEGMAANIRRFDDRLPELLAAITPDDILIITADHGNDPT
                     FRGTDHTRERVPLLVMNGAEGVEPRDLGVREGFSDVGASVAWWLRVEQEDLPGKDFF"
     gene            complement(11410..12051)
                     /locus_tag="VFJ72_11205"
     CDS             complement(11410..12051)
                     /locus_tag="VFJ72_11205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7480070.1"
                     /translation="MSDELHAEHRECRGDVEAERFWEEHYSRRERVWSGRANPVLADV
                     AGSLRPGAALDLGCGEGGDAIWLARQGWRVTAVDVSATALVRAAADAATAGVADRIDF
                     QQHDLARTFPGGAFDLVSAQYLHSPIEFPRARVLRAAASAVTPGGLLLIVDHASVSPR
                     SWAAPEETLALLDLSSDEWHTEKLEAREREATRPSGQRITVTDNIIAVRRLTP"
     gene            complement(12105..13097)
                     /locus_tag="VFJ72_11210"
     CDS             complement(12105..13097)
                     /locus_tag="VFJ72_11210"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7480071.1"
                     /translation="MSATERRDPAMYDVVVIGGGAAGLSGALMLGRSRRSVLVVDSGE
                     PRNAPASGVHGFLTRDGMDPAALLEAGRKEVRRYGVRVLDGRVASAAAVDGGFTVGLE
                     DGRRVGAKRLLVATGLVDELPDVPGVRERWGRDVLHCPYCHGWEVRDQAIGVLALNSW
                     AVHQALLFRQLTTDLTLFLHTAPRPTDEEAEKLAARGIAVVEGEVASLEIREDRLTGV
                     RLQNGEVIPRSAVVVGPRFAARAGMLAGLGLETTTHPSGMGERIEADATGRTAVPGVW
                     VAGNVTDPVAQVGAAAAAGAFAAAQINADLVAEDTERAVTAYRARREALTAGRR"
BASE COUNT         2312 a         4357 c         4439 g         2329 t
ORIGIN      
        1 gagcatggtc gcggccagca gcgccatccg gtcgtctgag cgccgcaaga agataaccac
       61 gccgatagcg caccatacca gctcgaagat gatacccagc gcggtattgt aagcggcgta
      121 gaagctcgcg gagagtccga gagaccgaag ctcccgcagc ctcgcgttcg tcaggagtcc
      181 gttatcggcg cacgcccgcg cgccgcgggt acacacgctc cgaagttcgt cgaagtacgg
      241 cgggatgctg gcgacgaaca gaccaaagct caacgcggcc acacccagcc acattactcg
      301 cgcaaagaga agccagtgtc cgcgcagagc cggtagggct tcggacctcg gctctgacat
      361 ttttggaagc ggccgggtac tcatgcgcct tctcccacgc ctttggactc ttgctctgcc
      421 ggagatctca gccacagcga gacgtgagaa ggctgcatgg tctcgcgcac tacgtctacc
      481 agatatccgc ttagggactc caggtccgtc tcatcgcgca gccgggcgcc gaacgcttcc
      541 agcgtcctag cggcgtcgta cttcctgcgg tagaagcgcc ggtccaccag atcctgcacc
      601 cggcgccgca ggggactaaa gagagcggct atcgccagag tcgaggctac aatcgccagt
      661 tgcgacttgt cgcccgtgaa ggcgcggaag acgtattgca aggcgaccac gccgccgaag
      721 tacaccagca cgagcgagac cgtcagggcc gcgtaaacta gagtccggtt tatgatgacg
      781 tctatgtcgt agaggcgata cttcacgacg gcgatgccga tagcgacggg gagtgcggcc
      841 gtgccgaggc ccccgatcaa ccatgccagg tcggggaaaa ttccgttgag cacgaggctt
      901 gagagcccca ggactatagc gtaggtaaac cacttgagct gctcgcgctc gtcgccccgt
      961 gagtgccaga aacgtactac cagagaggtg acggaagcaa gcatacagaa gatcccgaca
     1021 ggcccggcgg cgaccgagag aaagtcgaat acgggttgca aaccttcaat gccgaacggg
     1081 ttcttggcgg acgggtactc ggccagcgga cccggcgtga aagcgaacga gactaccgtc
     1141 aatgtgatga cgacggcgat gagccacagg accggacgcc agcgccggga gggaggacgg
     1201 ccattgggga agagcagcgg gacgaagacc agggagacaa agctgaggtt tccgagcagt
     1261 gagctgaacc agaccgcctc gatcccgagc tgcgagccgg ggtccgagag cagagcatgg
     1321 gtagcgtact gcgagacgaa cccgcttgcc gccgcggaga cgcccagggc gcagaagatc
     1381 cagcctagag cgttgaccgg atagcgcgag acgattacgg ccccgaccgc cggaaagacc
     1441 agcgcgatga ccgcttcggc gccccagtac ggatctgtgt tcaaccggtc gatatatagc
     1501 aggaacaggt acaacccgta tagtacgacc gagagcccgc ttagggacca ggagatccag
     1561 gctgctgcgc ggccgctcac ggtccctgct ccgccttcat tctcggcttc gcttcatccg
     1621 gcggccgcag ccagagcgat gcgtattctg gctgaagcgt ctcccggact acagagacca
     1681 gatcgttact caaaatctct agttccgtct cgtctctgag cgtctttccg aaagcctcaa
     1741 gcgtcctggc ggcgtcatac ttcctgcggt agaaacgcct gtctattacg tcctggatgc
     1801 gcctgcgcag gggaccgaac agcgcggcaa tggagagtgt ggagacgact atggcgagct
     1861 gcgactcgct cccggtgaag gtgcggaaga cgtactgcaa ggcgaccacg ctgccgacgt
     1921 acaccagcgc cagcgtcgcg gtgagcgaac catagacgag ggcgcggttt atgatgatgt
     1981 ctatgtcata gaggcggtgc ttcaggacgg caaagccgat ggcggtgggg atgcatgcga
     2041 agctgaccag gagcagatgc tgctctagcg aggcccacaa tggtgttgtg ccatcgctca
     2101 atagcgcatc cgatgcgaaa aagatttcgc tgaccaagag gaccaggtag ttcgcgccaa
     2161 tgaacgaagc ggcgaaggcg agccacttta tctgctggcg gacctctttg tcggagtgtc
     2221 tgtatcgtga gatgaggcta aaagccgagg ccaggatgca aacgggcagc agcaaaacaa
     2281 gtgctacccc cgcgtactgc aaccaggggt atccttcgat ccccaatgga ttgcttatac
     2341 ccccacggtt tggcaacggt ccggggccaa tatccagggc cacgctcgcc agcaagatca
     2401 cagctcccga gaaccaggcc agcggacgcc acctcctcga cgggagcctc ccgtcaggga
     2461 aaagcatgac catgaaggtg ccgagcagtc caacgggggg cacccaaatc gactgggtca
     2521 gagcgtctag catcaccggt cccgtgaccg aaccagtcgt ggcaaggctg taggcggtgg
     2581 acccttcgcc cgcgacgatg agcatccaga agaggccgac ggtcaggcag atccagccga
     2641 tcggattctc gggacgcttc gaggcgatca gggcgcccac gattgggaag gccaggaagg
     2701 gggcgaagat caacaggtcg ccgaagccgc cgctggggac ccgcgcggcg ggaacggcta
     2761 cgttccaggc aagcagcgcc agcgtgacgc ccgcgacaaa catggctacc gagaggccgc
     2821 cgagcgacca ggccagccag aaggtcatgc ggcgtctcat cgtttgccat cccgtgtccg
     2881 cagccagagc gagacgtgct ccggttgcat ggtctcccgc acgactgcta ccaggtgatc
     2941 gctcaggtcg tcaaggtccg tctcgtcgcg caaccgggag ccaaaagcct ccagcgtcct
     3001 ggcggcatcg tacttcctgc ggtagaagcg tcggtccacc agcgcctgca cccggcggcg
     3061 cagagggttg aacagggctg ctattgcaag ggtggatgct acgacggcga gctgtgagct
     3121 ctcgccggtt agggagcgca ggaccatctg gagcagaacg acgctaccga cgtagacggc
     3181 cgcgagcgag acggtaaggg cggagtagac gagggcgcgg ttgatgagga ggtctatctc
     3241 gtagagccgg tacctgagga tagctatacc ggcggcgatg gggatcaggc cgtagctgct
     3301 gatggcgatg tcgttgaaca gagaggccca gcccggcatc gcgctctccg acccgtaata
     3361 gaagaaggag acggtgtccg ctccgaccag gacgacgtac aggatcagag agctcgtaaa
     3421 ccacttgagc tgctggcgtt cctgtcctct cgaccggcgc tggcgcagag ccagcgagat
     3481 cagcgcggcc accaggagca gcacgggcag aggctgcacg atcccggcga gcggcctcag
     3541 gaggtggccg atgggtcctc ctattccggc gggattatct accttgaagc cgatggtatc
     3601 gtcgatccgg cccggcgcga aagccgacgc tgtagcgcca accacaacgt atccaacggt
     3661 cacccacgcg acagtccgcc agcgcggaga gagtagccta ccatcgggga acagcaggaa
     3721 aaggagtatg agcagcccaa cgctgaccgg accgggccag aaatagaacc acgccgccaa
     3781 cttccctccg ggtacggcgc cggggttgac ggccagggcg tatttagcgt agggaccggc
     3841 gaagtggttg atcgcccagc caacgttgac gacgcagaac agccagccta tagggtgctc
     3901 ggggcggcgc cagaagatca gggctccaac cgccggaaac gccaggatcg aggccgcccc
     3961 aactacggca tcgaccgcga agcccgaccc cagcgacgag gcaggacggt ccagagcctc
     4021 gaacagtatc ccgagcagca ccagcaccac ctgaagcgcc accgccgtcc agagcagcgc
     4081 cgcccatcgg cgcctcatcg ccgctccctt ccgcccgtct tccgcagcca gatcgagacg
     4141 cgcgcatgtt gcagcgtctc gcgcactacg tctgtcagat gttcgctcaa aggctcaagg
     4201 tccgtctcgt tccgaagcca cgcaagccga gtgaagagaa cagcgcgcct gcgcagcctg
     4261 tcagattgcg gtctctgact cccggcgatg ttgtttgatt tatccggcgg taggttcatc
     4321 caggcccttc acttcaggta gcgcagggcg gcgcgggcag ccaggtagcc gcacatgccg
     4381 tgcacgcccc cgccgggcgg cgtggacgac gagcagatgt acagtcccct ggccggggtc
     4441 gagtacgggt tgagccgggg cgtgggccgg gtgaagagct gcctgaggtc catgtagccg
     4501 ccgtttatgt cgccgccgac gaggtttgcg ttccaccgtt cgaggtcgga cgtactcatc
     4561 gccgtcttcg ccaggatgca ctccttgaag ccgggagcga agcgttcgat ctggtcttct
     4621 atcctcccgg tcatgtcgag ggtcgagccg ttcgggacgt ggcagtaggc ccagacggcg
     4681 tgcttgcctt ccggggcgcg cgtcggatcg aagaggctct gctgggcgag aaggacgaac
     4741 gggcgctcgg gatgctcgcc acgccctacg gctgcctctc cggcggatac ctgatccagc
     4801 gcgccaccga gatgcaccgt accggcccgc gaacatccat ccgccttcca ggggaccggg
     4861 ccgtcgagcg cgaagtccac tttgaagacg cccggcccgt aacggtagcg tttgagagca
     4921 ttccggtaac gttccgtgaa gtgctcgccc gcgatgtcca gcaggttgcg aggcgctacg
     4981 tcgaagagta ccgcgcgggt ccgcgggacc tcctccacgg acttcacccg gacgccggtg
     5041 tagacctcgc cgccaagaga gcgcaggtag gaggcgagag cgtcggccag cttctgggag
     5101 ccgcctttcg ggaacggcca gccgaaggcg tggcccagag tgcccatcac gagcccgaac
     5161 atggcgctgg ggcgttgatc caggggcagg aacgagtggg cggcgttccc ggcgaagagg
     5221 ccgcgcgcct tctcgccctc gaacaggctc tcggccagcg cgcgggcgga accgagggcc
     5281 cggagaccgt tggccgccag agcgaacgga tgccgcggca cgtttagcgt tccagatagt
     5341 agagaggcca gtcgggggcc gtcttcggcg aggggctgca tcagcttgcg gtacgcgttg
     5401 gcgtcgggac cgagtctctc ggcggtcgcg gctaccgagc gttcgagcac ggcggcggtg
     5461 ccgttgtcga agggatgggc gagggacgcg ggcgggtgga cccactccag gccgtgctcc
     5521 tccaggggga gcgacttgaa gaagggagag gcgaatccga gggggtgtat ggccgagccg
     5581 aggtcgtgga cgaaccccgg aagcgtcaac tccgccgagc ggactgcgcc cccgatggtc
     5641 tccccggcct ccagcacggc caccgagcgg ccgctgcgcg ccagctccac ggcggcggcg
     5701 agcccgttcg gacccgagcc cacgacgacc gcgtcgtaca cgccgcgctc cgtcacaacg
     5761 ctggccttcg ctgtacggtc gtcatcgggg gttctggcgg cgcatcaggc cgcgcatcca
     5821 gcgggtcggg gccgccatcg tgtagagcat ggagcgggcg cccgcgttgt gccagacgtt
     5881 catcgcctgc gaccactgga ccttcgggtc gacgcagacc ttctccagat cgaccggctc
     5941 gttcacgccg aagtgggccg ccagggactt gaggacgtgg gtccagatcc tctcctgctc
     6001 cgtggagccg accacgcgga agcccagctc gtagatgggg tcgttggcgc gggcgagcga
     6061 ctgtatctgg gccaccgtca cgccgtcttc gtcctcgtag gcgctgaagg tgttccagcc
     6121 ggactccggg tgcccctcgg gcgtcatcac ggtaaaggac tcgtcgtcgg cgtagatcac
     6181 gcgcaccccc gtgtgcaccg gcatcccccc gacggaggcg cttatgaaca gcacctctcc
     6241 gggcgctaca ccggccagcg atgggtagaa gcggctgttg gacggctgga actcggggaa
     6301 cttctccttc cagatccgca cgacttcggc gggcgtcgcc tccacaccgg tcagccggat
     6361 gcgatatgtc ttctgccaca tcttgccgaa gccctgaaga gcgccgacct cgcgccggcc
     6421 gtggacgttg acgttggagg cgccctccgg gacctcggag acctccagct tctctgtgta
     6481 cttcgcccag aaggcggcgt ctcggggttc gcgcgggtcc ttcttctccg gtggcggggt
     6541 tggcgtggtc atggttctac tcctttccct gattattccg gcgcagcggc gcggtcatcg
     6601 cgtgcagggc cgaacggatc gccgcgttgt gacggatgtt cttgtagtgt cgccactgac
     6661 gtttcggatc cacgcagacg acgcgggtct ccacctcgcc gggtgctccg aagtgggccg
     6721 cgaccgaccg gagcgtgtgc tgccaggtca tgtcctcgac cctgtgcagg gcgacgggca
     6781 tcatcagctc gtagagcggg tcgttcgccc ggatcaagag ctggacctgg gcaacggtgg
     6841 tcccgtcctc ggcgatgtgg gagctgaacg tgatccaacc agagaacgga tgcccttccg
     6901 gcgtcatata cgtaaaggat gtatcgtcgg cgtagataac tactattccc gtggagaacc
     6961 accggctccc cggcaggtcg ctgttgatca gcgcgacttc tccgggagcc aggcccgccc
     7021 tcgaagtcag gaagcggttc cccggcatcc agaacttcgg gaagtcctcc ttccaggtct
     7081 tgacgacccc ggccggtgtg gtgtcggcgc cttccaggcg cacccggtaa gtcttctgcc
     7141 acatcttgcc gaagccctgg agcggtccga cggtccggcg gccgtccacg ttcaggttca
     7201 cggccccttc cgggacctcg gagaccttca gcgtctctgt gtactccgcc cagtaggcgg
     7261 cgtctcgggg ctgtccttgc gctgtcatga tcgttcccct ctccgggagc cggcgttaca
     7321 tgccgtcggc cgcctttacg gcttcatcct cggcgtcgta gatggcgatg gcgtcgtcga
     7381 ggcgtgtgat ctggaagatg ctccggtagt gctcgctgag gccgtaggtc aataggcgct
     7441 gcttctcgcg gttgatgcgt atgagcagcg tcaccaggag accgatcccg ctactgttca
     7501 tgtactcgag tccctcgaag ttcaggatga tgacccgcgc gccatccgcg ctggcctggt
     7561 tgtaggcgtc catcaacaca ccctccgcga aggcggccag ttcgccctct atgtcgatga
     7621 tggcggcctt cctaccggcc ttcctcacgt tcatctttac ctgtgcctgg ggcatttcgt
     7681 tactcctttc gatggtgggc tacttcatga gacgggcgtt tccgtcaggg cgctgtcctc
     7741 gtccgggaag acgttcatga agtccgagag gcgggttatg ttgaagatct ccacgtagtg
     7801 ctcgctcagg ccgcaggcca ggaggcggcg ccccgaggcg cgcgcccggc ccagcaggcc
     7861 gacgatcagg gcgatgcccg tcgagttgat gtagtcgacc tgcgcgaagt tgagcaggat
     7921 gacctccggg tcttcggcct cggcttgtgc gtaggcctcg ttcagggcct ccatggcgag
     7981 gccgttgatc tcgccgcgca ggtcgaggac gatggccccc ggttcctggc ggacctctac
     8041 gtccaggtgg cgcgcagcca tcatttccct ccttttgctc gtccatcgag gcgtacgatc
     8101 agctcgatgg tgtggtggtt ctcgtcgccg ctgacgcgca tctcgtcgac catgttcttg
     8161 atcaggaaca acccccagcc gcgaggcgtc tgcatacctt ccagctttgc ctctatgtcc
     8221 ggcgtctccg cgtccggctc cggcggcggt ccgcttccct cgtcggtgat cctgacggag
     8281 aggtccgcct cggtggccga cacctcgatg agcacgggga cgtccgcccg gtactggttg
     8341 ccgtgttcca tcgcgttcat cgtcgcctcg gcaacggcgg tcttcagccg gtccagggtg
     8401 ggaggaggca ggccaagctc ccgcaccgct ccggcgacct cttccatcgc ccggcgctcg
     8461 ttgcccggct cgctcgggac actaagctcg gccagaactc ggcgggcgcc gtttcgctcc
     8521 gtcttgccgg attgtttggg agcagtcaag atgcctccga tctctccagg gtgaccagtg
     8581 tgatgtcgtc ctcctgctcc cagccctggc cggtgaaacg ggccagctca gccaggagga
     8641 agctcaccag cggcgaaccg cccgaacggt gcgccccgac gaggccctgt agccgcggga
     8701 acccgaacat ctcccgctgt gggtcgtgag cctcgaccag gccatcgctg tagaacagca
     8761 cgccctcgcc tctctccagg acgatctcct tctcctcgta acccatgccg ggcatgaggc
     8821 cgagcggcat ccccgtggcc cgcagttcct ccgcgcctcc agaacggtct gccctacgcc
     8881 ggtagggcag gtcgtgtccg gcgttcgcgt accggagttg tccggttcca gggtcgagga
     8941 tcgcgtagag gcaggtgacg aacatgtttg gcgggatgtc cgggaccagc acgtcgttca
     9001 cgcgccggag gacctcgccc ggagtatcca ggcgctgggc ggcggcgcgg agcatggtgc
     9061 gggtcgtcgc catcaccagg gccgccggga cgcccttgtc cgtaacgtcc ccgacgacga
     9121 ggcccaggtt cccgtcctca agctccagga agtcgtagaa gtcgccgccg acctcacggg
     9181 cgggctggta gtaggcttcc acctggtagc cttcgatctc gggcacggat tttggcagga
     9241 gggtctgttg gatcaggcgc gccacccgca gctcctgctc gatgcgctcc ctctcctgcg
     9301 cctctagctg ctgctggcgc accagttggg cgacccggac ggccggagcc gtctgggtgg
     9361 cgaggtcgtt caggagcttg cggtcgtcgg cggagtattc ctgctgcgag aggcgcggcc
     9421 cgaggttgag cagcccgatc aactcgccct ggctcaccag cgggacgagc atcttcacgt
     9481 ccgcggcttt catcgcacgt aacgccggcg agtcgaggtc taacctctcg acctcgacca
     9541 cccccgagac gcttgcgagg taggccagga tcggatcgtt cggcgctatc tcgatctcgg
     9601 gagcctcaac tacggcgaac tcttctggag cctccgtcag ggtggtcact ggcgcctcgt
     9661 cctcgcgccg gaacatcgtg cgccaggtgc gtcctttttt ctgttgcgtc gtcatctgac
     9721 ctccttcacc gccatactag gtagcgagcg tcttctaaac gttacgaaaa gcgttacgaa
     9781 acagcgtccc tgaaacaatt ctcgaatttc atgcccccat ctttcctttc ccggctattt
     9841 aaagtatgcg gccgtaacgg tccacgctcc agttccatcg cgctacgtag cgtcaaagat
     9901 ggcagaccga agcagagagg gcatcgtccg aatggactat tttgccgcgt tgtcagcctc
     9961 ctggcatggg cctgccggag gggcctcttg atcttcacga gaagcgttac ggggcggacg
    10021 gagatccaga gagccggaat cccgagccca aagtggagag acggcgcgga gcggaaccgc
    10081 ttctagctct ttatccagaa gtaacccgtc cgggcgctaa aagaagtcct ttccgggtag
    10141 atcttcctgt tcgacgcgga gccaccaggc gaccgatgct ccaacgtccg agaaaccctc
    10201 ccggacaccg aggtctcgcg gctctacgcc ttcggctccg ttcatgacga ggagcggcac
    10261 gcgctcgcga gtgtggtccg tgccccggaa ggtagggtcg ttaccgtggt cggcggtgat
    10321 gatgaggatg tcgtcggggg taatggcggc cagaagttcc ggcaaccggt cgtcgaagcg
    10381 acggatattg gcggccattc cctccgggtc gcggcggtgg ccgtacttcg cgtcgaagtc
    10441 cacgaggttg gcgaagatca ggccggactc gacccggccg agggcgtcga ggatggcgtc
    10501 caccttcgcg gagtcgtcgg gcggggcggg gaggtgttcg gtgatgccct ggccgtcgaa
    10561 gatgtcccgg atcttcccga ccgcgacgac ttcgtggccg gcgttcttta tgcggtccat
    10621 gtacgtctct gacggtgggg tgatgccgta gtcgtggcgg ttctcgttct cgcgctcgta
    10681 ggctccggct tcgccgtgga agggacgggc gatgacgcgc tcgacgagta tctgcccctc
    10741 gcctatgagc atcttgtggg ccttctcgca ggcttcgtac agttcttcga gcgggatgac
    10801 gccggtgtgg gcggcgatct ggaagaccga gtcggcggag gtgtagacga tccaggagcc
    10861 cgtcttctcc tgatcggggc caagctcctc gatgatctcc gtcccggaag cgggtctgtt
    10921 cccgattacc tcgcgctccg tctcgtcctc gaaccggagc aggatctcct ccgggaaacc
    10981 gtccgggtag gtgggcaacg ggtcgtccag cacgatcccc atcatctccc agtgcccggc
    11041 gagcgtggcc ttcgccgccg ccttctcgac catcaggccg tgtgaggctc cgggcgactt
    11101 tacgtgcggg acgccttcga tctcgatgac gttgccgagc ccgagggctt gcaggttcgg
    11161 cgcgtcgagg ccgccgaccg cgcgggcggt gttcgccagg gtgttcgcgc cctcgtcacc
    11221 gaagtcggcg gcgtcggggg cgttgcccgc gcccagcccg tccagaacga ccacgattgc
    11281 cctgcgtccc atccctgtcc tttcgtcacc ttttcgtcgc gggtcatatt aacttcagat
    11341 ccaacctccg aacaggcgcg agagaccgaa cacctcgttg caaggcgacg gatcggccgg
    11401 gacggccgct cacggggtca ggcgacggac cgcgatgatg ttgtcggtga cggtgatcct
    11461 ctgcccgctc gggcgggtag cctcgcgctc gcgggcttcg agcttttcgg tgtgccattc
    11521 atcggagctc aggtcgagca aggccagcgt ctcctcgggg gcggcccacg accgggggct
    11581 gaccgaggcg tggtcgacga tcagcaggag gccgccgggc gtcacggcgc tcgccgccgc
    11641 ccgcaggacg cgggcgcggg gaaactctat cggcgagtgc aggtactgtg ctgagaccag
    11701 gtcgaaggcg ccgccgggga aggtgcgggc gaggtcgtgt tgctggaagt cgatgcggtc
    11761 ggcgacaccg gccgtggccg cgtccgccgc cgcccgaacg agggccgtcg cggatacgtc
    11821 gacggcggtc acacgccagc cctgccgggc gagccagatc gcatcgcccc cctcgccgca
    11881 gccgaggtcc agcgcggcgc ccggacgtag gcttccggcg acgtcggcca ggacagggtt
    11941 cgccctcccg ctccacaccc gctcgcgccg gctgtaatgt tcctcccaga accgctcggc
    12001 ctccacgtcg ccgcggcatt ctcgatgctc tgcgtgaagt tcgtcactca atgttgtatc
    12061 tcctctccgt cgcgttgctt ttttgctgtt tcgtaccgag ggggctagcg ccggccggcg
    12121 gttagggctt cgcgccgggc gcggtaggcg gtcaccgcgc gttcggtgtc ttcggcgacc
    12181 aggtcggcgt tgatctgggc cgccgcgaaa gcgcccgccg ccgcagcggc cccgacctgc
    12241 gctacgggat cggtgacgtt cccggccacc cagacaccgg gtacggcggt gcgcccggtc
    12301 gcgtcggcct cgatgcgctc gcccatgccg gagggatggg tggtcgtctc cagtccgagg
    12361 ccggcgagca tcccggcgcg ggcggcgaag cgcggcccga ctaccacggc gctacgcggg
    12421 atgacctcac cgttttgcag ccggacaccc gtgagccggt cttcgcggat ttcgagcgaa
    12481 gcgacctcgc cttccacgac cgcgatgccg cgcgcggcca gcttctccgc ctcttcatcg
    12541 gtgggcctgg gcgcggtgtg caggaaaagc gtcaggtccg tcgtcaactg ccggaacaat
    12601 agcgcctgat gcacggccca cgagttcaat gccaggacac cgattgcctg atcccggacc
    12661 tcccacccgt ggcagtacgg gcaatgaagc acgtcgcgcc cccaccgctc ccggaccccc
    12721 ggaacgtccg gcagctcgtc taccagcccc gtggcgacca gcagccgctt cgcgccgacc
    12781 cggcgtccgt cctccaaccc cacggtgaag ccgccatcga cggcggcggc cgaggcgacg
    12841 cgcccgtcga gcacccgcac gccgtagcgg cggacctcct tccgtcccgc ttccagcagc
    12901 gcggccgggt ccatgccgtc ccgggtgagg aacccgtgga ccccggaggc gggcgcgttg
    12961 cgcggttcgc ccgagtcgac gaccagcacc gagcgccggg atctaccaag catcagcgcg
    13021 ccgctcaggc cggcggcgcc gccaccgatt accacgacat cgtacattgc gggatcccgc
    13081 ctctccgtcg cgctcatttc gtcctttctc atgtattttt gggacaatgt ttcgacacaa
    13141 attcagtatc gaaggcgaat gtgaggctga aaagggtgaa tgttcgggtt ttatgagacg
    13201 atcttcggta atatcgtgga tgttgggccg tcggcgtcga tagtcgcggc caccggggaa
    13261 agcggggatc gggttgggta cgacgaggac atccggcagg cggagcggcg ggctgcccat
    13321 ccgcacctac gaggcggtgc cgggggtgcc gcccgtgcgc gtcctgagat ttccctaccg
    13381 ggagcctccg cggggtgggt cgttggtggg acaggcccac acccatgact tcctcgc
//
LOCUS       DASSMB010000189        39375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1312163, whole genome shotgun sequence.
ACCESSION   DASSMB010000189 DASSMB010000000
VERSION     DASSMB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 39375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39375
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312163"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFJ72_11215"
     CDS             <1..283
                     /locus_tag="VFJ72_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7480072.1"
                     /translation="LPMIIVVYNDAAYGAEVHHFGPMGRPVDLTQFPDTDFADLARAA
                     GAEGITVRTPEDLAPLKEWLGRRDGPFVIDAKVNPGVRAEWLEEAFRAH"
     gene            299..1081
                     /locus_tag="VFJ72_11220"
     CDS             299..1081
                     /locus_tag="VFJ72_11220"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7480073.1"
                     /translation="MSENPVASLIEALSGGSVEIVDLTQPLNEKTPVIQLPEPFVNTP
                     GLKAHEISAYDDRGPAWAWRWLEIGEHVGTHFDAPTHWISGRDGLDVSQVPPRHLVAP
                     AVVIDKTAEVEKDPDYLLTVEDIHAFEDEHGPLPDGGWILFRTGWDARAQDEEAFLNA
                     SAGQPRSPGFDAECARWLAEESPLVGVGVETVGIDAGAAGGFDPPFPVHYYLLGAGKY
                     GVTQLANLGSLPSTGTLLMIAPLKLTGGTGSPVRALALVPRS"
     gene            complement(1104..2117)
                     /locus_tag="VFJ72_11225"
     CDS             complement(1104..2117)
                     /locus_tag="VFJ72_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7480074.1"
                     /translation="MDASEVSPRDSLWRMVNGYQVSQAIHVAATLGIADLLKDSPRSV
                     DDLAEATGTHAPTLYRLLRALASVGVFVEKEGCFGLTPLAEYLRSDVPGSIRAWATFI
                     GRPYTWSAWAHLIDSAKTGETAFPMLYGMSVWEYRTSHPEESGIFDAAMTHLSAGAAE
                     AIADSYDFSGIGVLADVGGGQGEILAKILAANPDLLGILFDQPHVVAGAKDLLERMGV
                     AGRCEVVGGSFFEAVPGGADAYLFKSVIHNWDDETAAGILRKCREAIPDDGRLLLVER
                     VFGPPNEPDPMKFMDLMMLVMPGGKERTEAEFRSLLAQAEFELRNITATGSPYDIIES
                     VPV"
     gene            complement(2292..4286)
                     /gene="ligA"
                     /locus_tag="VFJ72_11230"
     CDS             complement(2292..4286)
                     /gene="ligA"
                     /locus_tag="VFJ72_11230"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7480075.1"
                     /translation="MAEKTDIRGRIEELREQVRYHNRRYYIEDAPEIPDAEYDALYSE
                     LESLESANPELVTPDSPTQRVGGDPLEQFEQVRHAVPMLSLANARKVEDLREWDARVR
                     RLLGEDETPRYVTERKIDGLAISLRYENGRLARGATRGNGTVGEEVTANLRTVRSIPN
                     KLDDDPPQTLEPRGEAYMTLAEFEKLNRRQEEEGKPPFANPRNAAAGSIRQLDSKITA
                     RRPLTVYLYGIGEGGDLYESHSAALDALKSYGLRANPYRLHEDIESVIEECEKAARER
                     ESLPYQIDGVVVKVDSRDQQRALGSVAKAPRWAIAYKFEPLAGRTKLNDIIVNVGRTG
                     ALTPQAVMEPVNVGGVTISRATLHNEDYIKEKGILIGDKVVIERAGDVIPQVIKPIAE
                     ERTGAEREFVMPTYCPVCGEPVSRPEGEAVTRCVNARCPAQALEHIIHWAQKSAMDID
                     GLGEKLATRLFDLGLIKDAADIYELEVDQLVPLEGFGEKSAENLIRSIHNSKQQPFSR
                     VLYALGIRHVGSVTAQLIAERFSGEDLLRGVLVEQLVEIDGIGAVVARAVVEYFDLED
                     NQELVERLMRLGLDFERESGRPSEGPLAGKRVVITGTLSQPRSYYVERVEAAGGTFTS
                     SVSKNTDYVLAGEEAGSKLEKARELGIPVLDEASFEELLS"
     gene            4362..4739
                     /locus_tag="VFJ72_11235"
     CDS             4362..4739
                     /locus_tag="VFJ72_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480076.1"
                     /translation="MGGRPKHRQLQSREDFERALQEEISVSNQFDLPLCVLVAVMEGG
                     WDPESEGRAVGALRLADLVSRTDGPDLIVVLSNTALENARAVERRLRQILPEVGIGIS
                     ELTPGDTAQRLVERARQAALRNL"
     gene            complement(4736..5302)
                     /locus_tag="VFJ72_11240"
     CDS             complement(4736..5302)
                     /locus_tag="VFJ72_11240"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HET7480077.1"
                     /translation="MMLRAGTILLLILLAAGCAAGGGSGSSPEGSNTTSQESTAPSTT
                     QTQETTESSMERNEISLEQIAANAPGQGPKRPKVVVASSADALSKALGPKAKIPDSGE
                     GIYLAAFWGQKPTGGFSMAARSARGEGGKVMVGLALKRPPKDAFVAQVLTYPYVVAVV
                     RGAPADAEFVFVDQGGRKLGWPLERAGG"
     gene            complement(5338..7242)
                     /locus_tag="VFJ72_11245"
     CDS             complement(5338..7242)
                     /locus_tag="VFJ72_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7480078.1"
                     /translation="MVRRRDRDPYAESFRERHVVGINSIKRWTRRILLVLLLYALYLL
                     SIWMIFPWGQGGAPPYSVAAANSFIPALLVKLFGLGFTLFFAMMFLIVQFVALFWFLA
                     RGRTYVIYPGEYDATFDDVRGQKAAVEATREVVRLFQGFKKFRKMGGYPPQGILLEGP
                     PGTGKTLLSKAIAGEVNVPFIYASASSFSSMFMGIGNLKVMRLFTKARKFSERYGGAV
                     IFMDELDAIGSRGGVAQTMEPYPFHEPFRHGLEDRLLAGPADAQKRSGFVDRVMGGMG
                     MGGMGMGGMLVNELLVQMDGMEQQKGIRRFFRRLIPLFIRRNLKRKIPSYNILVIGAT
                     NKADSLDPALLRPGRFDRRLHVGLPDKNGRADIADYYLKKVHHEDVDLEKLAKATVGY
                     SPASIKNIINEALIFALQDGRDSLGWEDIWAAKLTDEIGLKQPVEYNEEEKVKIATHE
                     AGHAVASWYLEREELQMQVITIIKRENALGLVSYQEREERYVRTRSDVIARIKTALAA
                     GAAENIYFGETTTGVSADLRQATWAAATMVGMFGMGKGLYSFGTLPNEMAGSSIGALL
                     RDSGVREESNSILAEARREIEELLREKAPIVEGVRDALLEREEIVGDEIDRLFTELEA
                     GGSRQSSGVV"
     gene            7330..7983
                     /locus_tag="VFJ72_11250"
     CDS             7330..7983
                     /locus_tag="VFJ72_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480079.1"
                     /translation="MHYTTLPMRSWIWKILIISIVVACALAPVACGANQNTSSRDPSG
                     KEGSTLATTVPEDSAGVQEGAGAVAALSGVQKADQAAAEWQSDAELYAIASLKPQVDA
                     EGRAPAWLYTYVSSSRKAVVSVSVSGGKVADTPEQALPDEQIEDISNHTLPPPARLLD
                     STEAVKKSGKFLDTLKSEPGLDASAGLDSFSTGKPVWIFSTAKGDKRVEERVSATGS"
     gene            complement(8019..8324)
                     /locus_tag="VFJ72_11255"
     CDS             complement(8019..8324)
                     /locus_tag="VFJ72_11255"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7480080.1"
                     /translation="MERSGPEPHNPYTALLGYTLLDASGEEFGRVDDIVYDAPTDVLK
                     YVVSGGHTIPADEIEVDPDRERVHAPYERETIESAPTLEDPSGAFDERLREHYSEPG"
     gene            8378..9370
                     /locus_tag="VFJ72_11260"
     CDS             8378..9370
                     /locus_tag="VFJ72_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480081.1"
                     /translation="MNAKTNHRRNARTLKTLSLLYLGLTVVSLLFVHSDSYEPAVSYV
                     LLYFAVSIGVVTAVALLLLPTQQYTGGFSTGAYALYAAFVAMMVFFSGGVSSELYVLY
                     FPLLLASALHGHWRMVMMAVIFTLFTYFLAATPDIMNEGAATGAAALAFFRLAVFALT
                     GVFLVAAARNFAGAADSEDYDYDTDEDGSMLRERVEAELAARKGVPVAVVLVDPGRKV
                     EDVELLLERVRGRIGDPVLLGEGTCFGIVLSGMDDRGVESAARRALAAASSLGAEETR
                     AGAAIYPRDARSANDLLVSAGQALEAAFEIESPSAIVLAGRGTPRAESRYRAAR"
     gene            9491..10267
                     /locus_tag="VFJ72_11265"
     CDS             9491..10267
                     /locus_tag="VFJ72_11265"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET7480082.1"
                     /translation="MGRFFRAIRGFFGSFLSGVEQRNPEILLENAVQDELENLKKLQV
                     AAARTMAYEKMLANDGASKQKIIAAKERQARELAARGQRDAAIEVVQQKQEAQTQLAD
                     IESRLEEARNNSQEMEQAFRDQERRYNDVVRERAALMEEHQRSRALRTANEARASISL
                     SDSSRDLDKARQAIRQSSYEAQAVGELGTSETERQIAAAEVDIKRLDAERELEMMELQ
                     MGLRDADSLEAPANEETIVEQPVEGVGEAEPAEPEERPRG"
     gene            10260..11063
                     /locus_tag="VFJ72_11270"
     CDS             10260..11063
                     /locus_tag="VFJ72_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480083.1"
                     /translation="MGSLEVRRRQGLSISAARLIPAGSFAGLAGLAALETHYFLAGLL
                     GVGAFALGRKLLNRQENRRRELRRRAVSNVRELGRVAREDRVAASQMKRLMALQNGLL
                     ESWELLPEEYRPLLDDDIFNIVGEVEGTARLARRRTALRKHLQSLDRRGIERRIKSLE
                     RDLAGLEPGSPLRAPFESALSGRRDELAGYGELMDGISMINAQLEGAESLLGGLRGEL
                     LALDTSLAPGSLESGLVHLKERVAYFRRGLDEVTRSVETLPETTVEVSV"
     gene            11063..11788
                     /locus_tag="VFJ72_11275"
     CDS             11063..11788
                     /locus_tag="VFJ72_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480084.1"
                     /translation="MSEDRSRQSFFSRMFAPVDDEGTTTYSRDDIGEEHFEQPEGESV
                     DEPRGFTVERAADIIRELPPEVPRRSAVRIVRQTLIAAGISVDDLGRSTRVRESKLNS
                     EIELRQQRIEELQQNTDEVIRDLEDQIKKAREARDFGVAEEERGISEAREGLEDVEKV
                     RDFFDLPRGETRSTPFGDQSGEATTGENATYPVGDETQVMDRSEMDQPEDPANMDDTQ
                     ILRPRGPLSEEWEARRDTENRQP"
     gene            11785..12273
                     /locus_tag="VFJ72_11280"
     CDS             11785..12273
                     /locus_tag="VFJ72_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET7480085.1"
                     /translation="MSIPRRLGRMARGFVSNIQEDERLRESLRETIRTGRERSENLRE
                     AFGAAWRGAAEEWRNAEERRAAEEEVGERIAGQERSERRFSPGTFVPRIYPLNVLSAY
                     NRLGLEPGAPLEEVSRKRRELIKKYHPDRFTDPDKRIRAERLSAEINAAHDTIERHMS
                     KS"
     gene            complement(12286..12888)
                     /locus_tag="VFJ72_11285"
     CDS             complement(12286..12888)
                     /locus_tag="VFJ72_11285"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET7480086.1"
                     /translation="MPVVLFALLALPIIPQAESSSVDLGTAEVQTAGKLLGENAAPLA
                     EPPESPPKTPEKAATGAAQAMTLTIPKLGIKDLDIPTAASQAVLDREGIIHLQNTGVP
                     WQRGSNTFIVGHALGFMQTRVPYVFYKLDKMRPGDEISVKDTTGKEYVYRVYDLMTVR
                     PADYWVTYPVAGKTTISLQTCTPIPTFENRLIVRGELVSG"
     gene            12968..13801
                     /locus_tag="VFJ72_11290"
     CDS             12968..13801
                     /locus_tag="VFJ72_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7480087.1"
                     /translation="MKIEPLIEEFGAHLADDEHTSPRTVEFYEADLREFARFLAGNDI
                     REIESVGVGSVMEFRNHLLEGKRKRFTVYRKDAAVRRFLDWVRTATDADLDFDPIEPM
                     HQPLDQQIVFLENEEAEQLLSFPTQERDAARDAAVIRLMLDTGVTVSEIRGVLRSDVD
                     LDAAQINLGGPGSHLAPRTTRLSDTTVEAVREYLEMRADETPELIVGRAARPVTTSKS
                     LQNAIWKRCEQVGMWRVSPMNLRHTFAIRLLRRGATLNELKDALGVRDTTNIGVYKKF
                     V"
     gene            14419..16869
                     /locus_tag="VFJ72_11295"
     CDS             14419..16869
                     /locus_tag="VFJ72_11295"
                     /inference="COORDINATES: protein motif:HMM:NF013600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480088.1"
                     /translation="MNGKIPDVLLDVPVVKIDEINFELNDLRAKVNLFAKVLDLVELS
                     VGVDAYLGRVKLVIKGVEAQALLKVRLDNVTAILDRVLTTIDRNPQIIEKLVEGVSSA
                     VSDIGAGAGDLVGEGLNSAVEDIGAGAGDLVGEGLNSAVEDIGSGAGDLVGEGLNSAV
                     EDIGAGAGDLVGEGLNSAVEDIGAGAGSAVEDIGAGAGDLVGEGLNSAVEDIGAGAGS
                     AVEDIGSGAGSAVEDIGSGAGHAVGEIGEGAGSAVEDIGSGAGHAVGEIGEGAGSAVE
                     DIGSGAGHAVGEIGEGAGSAVEDIGSGTGHAVGEIGEGAGSAVEDVGSGAGHAVGEIG
                     EGAGSAVEDVGEGAGSAVADVGEGAGEAVESTGQAVESVGEGAGSAVEDVGEGAGSAV
                     KDVGEGAGEAVESTGEAVEDVGSGAGSAVEDVGEGAGQAVESTGEAVENVGEGAGSAV
                     EDVGGGAGQAVGEVGQGAGEAVGEVGQGAGQAVSEVGEGVGETAQQVGEGVGQTAQQV
                     GEGAGQAIGEVGQGVGQATEQVGEGAGQAVQEVGEGAGQAAGQVGEGAGQAVGEVGQG
                     AGQATQQAGEAAGQVAEGAGQAAQGVGDAAGQAAQGVSDTAGRATEQVGQVAQGATDA
                     VEQTAEGATDAVEEATDTAGQVAESATDAAQGVTEQAQDTVGQATQQATDTAGQVGQQ
                     AGETVEGAVDQAQETAGGAVDQAQETAGQAVDQAQDAAGQAADQAGQAAEGAQEAANQ
                     VVERTVDESGNILETVTNENGEVIGEDLVGTVGDLPIEEEYIDDQGRVVSRAKDDSGN
                     VYEQVFDDEGNVVEVRSA"
     gene            16931..17809
                     /locus_tag="VFJ72_11300"
     CDS             16931..17809
                     /locus_tag="VFJ72_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HET7480089.1"
                     /translation="MAEDQVNEALEKLNRGIQESKQMVQQQTMQLAQDFFSDSVEELK
                     RHIKENRSTLEGLPGQIPGGQEESFQMLFQELMDNYQAIEQALDEAEKNVSNLDTDSL
                     VQQGEVEATDAARREARELGVDLTEIEGTGTDGRITVDDVKKASDSAREEAGEAAGGA
                     ADEAQKASDAARRRAEEMGVDLSQIEGTGSGGLITVKDVTSLAEGAGDQATQAAGQAA
                     DQAQDTAEQTAAQADGAVGQAQGAGQAADGGNGSEGPKATNAAQRRAEEMGIDLSTLK
                     GSGAGGLITVKDVVKS"
     gene            17814..18116
                     /locus_tag="VFJ72_11305"
     CDS             17814..18116
                     /locus_tag="VFJ72_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480090.1"
                     /translation="MREKRPDVSETPDIEITAGVKTRRLRFEKVPETEVRFRGYPDRE
                     SVSGTDRENLPDRVERGVTYRDARVRLRIASEIAESAFETGDREPGHGSEDPKQSV"
     gene            18141..19253
                     /locus_tag="VFJ72_11310"
     CDS             18141..19253
                     /locus_tag="VFJ72_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HET7480091.1"
                     /translation="MTENAQNLAQQIQEQAVNSAKDFYGNALGEVKGQLESSRSQLEE
                     LLEQLPEGQDEARSQIQELIDSYESISNALDEAAQTQGVEDTVNQAVQQAQETAEGAA
                     QQAQDTAQGAADQAQDAAGGAVDQAQQTAGQAAGQAQDAAGGAVDQAQEAAGQATEQA
                     GQAAEGAQGAVGQVTDQVGQVAEGAQDAAGGAVEQAGQVAENLPGGQLLSETVNDAGQ
                     TVQRVVDESGDIIETTLDEAGNLIDEDIAGKVTDLQPAEGYEEYQDEQGNTIRTVTEE
                     SGGLIKLQLGPDGSLLNLEIPPSTEEVTEEVEETTQQTQQTQQGGGGQQQGGEEPDAT
                     EAAKQKAEELGVDLSQIEGSGAGGRITVKDVAGAAG"
     gene            19398..19685
                     /locus_tag="VFJ72_11315"
     CDS             19398..19685
                     /locus_tag="VFJ72_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480092.1"
                     /translation="MAESRGNGKGGVQETKAWLVKSARMLAMSEKAGVSEPMDVTGLA
                     LAAFEDMPLNQALVFIRTNEQNIRDLAWAFKNSSSAEEFEERVREIKHLSD"
     gene            20268..20822
                     /locus_tag="VFJ72_11320"
     CDS             20268..20822
                     /locus_tag="VFJ72_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480093.1"
                     /translation="MSVRDQERRQREAERRISQAMKRADREVRDAMEARAVAADRRKE
                     QVKRRIEIGDVDPRPLPGLREDSMESMPSMESSESIKSMPSMESADYASALTAEEANA
                     GGNMMQDMPLEGKMPIDPARAEEELAQELTEETGSGGDVHLHAERKADEMANQMTEDT
                     GAGGDMELHAERKVEQMGNEMFED"
     gene            complement(21031..21303)
                     /locus_tag="VFJ72_11325"
     CDS             complement(21031..21303)
                     /locus_tag="VFJ72_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480094.1"
                     /translation="MTQGNQQQQGQQGQQQSQGQQAQGQQQQGQQQQQQSPVQQLASA
                     LTAIRKVQNLMELNYPQQGDAIQVQREAGDLVWNVIQQMQKQQQGS"
     gene            21452..22126
                     /locus_tag="VFJ72_11330"
     CDS             21452..22126
                     /locus_tag="VFJ72_11330"
                     /inference="COORDINATES: protein motif:HMM:NF014832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HET7480095.1"
                     /translation="MSRSRRPRDEEHPAQDSEERPVGEQPRQIEEADVLLDVPELNVE
                     EFSLQAEQVRVRVSFGAELADMVKINVGLEAEVDDFELKAKGIEAQAQLKARLGNVRE
                     IFSQVLSTLDNNPSLIQDLLGAPDRKGDEPEGEPAEPPRRALEESPPDDSDASDGGDT
                     RVNARRDGGARLTEDSATNGPRKSDATEAAEKKARQLGIELGSLEGTGVGGRVLLKDV
                     VEAARK"
     gene            22194..22517
                     /locus_tag="VFJ72_11335"
     CDS             22194..22517
                     /locus_tag="VFJ72_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480096.1"
                     /translation="MAKDFKQSSAEILSELLSDERVKRVREYVQGEDFKADRERVRKN
                     AADRLKNIRERYGAKRRDPETAAREKELTLRLAEVEAEAAEIRIRLSELLEEEGKLRS
                     ALDEL"
     gene            22661..27148
                     /gene="gltB"
                     /locus_tag="VFJ72_11340"
     CDS             22661..27148
                     /gene="gltB"
                     /locus_tag="VFJ72_11340"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HET7480097.1"
                     /translation="MTPKTGLYDPLYEHDACGFGFVARIDGRRTRETVEEGLEVLHNL
                     EHRGTTGADPETGDGAGILTQIPDGFFRRECKELGIELPPAGGYAVGVVFEFGEAEGL
                     DCEGRLAEICAEEGQKLLGFREVPVVPEAVGKIARSVMPRVRQFFIEGHAGDEDAFER
                     KLYVIRRRLHKAVEGGHGCYVVSLSSRTVIYKGLLKGEQLPRFYPDLGDPAFASAIAL
                     VHERFSTNTLGSWELAHPYRYVAHNGEFNTLRGNVNWMRARESRLQSDLFGEDLKKIS
                     PVIQTGQSDSASFDNGLELLHLAGRSLPHAVSMMMPEAWENDELMDPERRAYYQYHSA
                     LMEPWDGPAAVAFTDGRLIGATLDRNGLRPARYSVTKDGRVVMASEDGALRIPAEEVI
                     ERWRLQPGKMLVVDTERHELLHDEEVKKPLFARHPYREWLEDGEIHLNELPEADSERR
                     PESASLFERQQAFGYTIEDQRIILAPMGMDGKEPDGSMGTDTPLAVLSERPQMLFSYF
                     KQHFAQVTNPPIDPLREELVMSLKMSLGPEQNLFDETPEHCRRILIDQPILTAAELEK
                     IRHLGTERASTTTLSTLFSVSSGEKGMEDALTSLCERAVSAVRGGSAVLVLSDRGVNP
                     MQASIPCLLATSAVHHHLVRAGIRTATTLVVETGEAREVHHFALLVGYGATAIAPYLA
                     FETIEDLVLSDHLMEGVSPEEAAKNYVKAIGKGLLKVISKMGISTLFSYCGAQIFEAV
                     GLDKDFVEKHFTGTASRVGGVGIGALAREVLERHRNAFGGTEDNAEELEIGGEYQLRA
                     QGQYHQWNQKSIPALQRAVKTNDFQTFKEFTRHFDEQSARFSTLRGLFDFEEAPIPLE
                     EVEPAKEIVRRFVTGAMSFGSISKEAHETLAIAMNRIGGKSNTGEGGEDPARFTPDAS
                     GDNRRSAVKQVASGRFGVTTEYLVNADVLQIKMAQGSKPGEGGQLPGHKVSEDIAKVR
                     YSTPGVGLISPPPHHDIYSIEDLAQLIHDLKNANPRADVSVKLVAEVGVGTVAAGVAK
                     AKADHITISGHDGGTGASPLSSIKHAGLPWELGLSETQQVLVQNNLRGRVILQTDGQL
                     KTGRDVVVAALLGGEEFAFSTAPLVATGCIMMRVCHLNTCPVGIATQDPELRKRFTGT
                     PEHVVNYFFFLAEEVRELMARMGFRTFDEMVGRSDMLKMREAIEHWKAKGVDLSAILH
                     QEEALEGGEIRHSETQDHGLEKSLDNELIERCMPALEEKEPVRFSQEVANTHRTVGGM
                     LSGEIARRYGNDGLPEDTIRIDMRGVGGQSFGAWLAKGLAFSLEGTTNDYVGKGLSGG
                     KLAVFPSRVAAYDPSRSVVVGNVALYGATGGEAYFRGFAGERFAVRNSGAHAVVEGVG
                     DHGCEYMTGGVVVVLGPTGRNFAAGMSGGVAFVLDEEDRFEKLCNVDMVSLEAVESEE
                     DEALLRSMVERHLEWTESDAARRVLEDWEELLPKFIKIMPNDLKRVLRERQEAELEVA
                     R"
     gene            27148..28575
                     /locus_tag="VFJ72_11345"
     CDS             27148..28575
                     /locus_tag="VFJ72_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HET7480098.1"
                     /translation="MGDPKGFLKIGRKPTPKRTIEERVQDYRYIYEPMPEDELKGQAS
                     RCMDCGVPFCNTGCPLGNLIPDWNDLVYKDHWKTAIDRLHKTNNFPEFTGMLCPAPCE
                     AACVLAINDKPVTIKEIELSIINRAFDEGWVVAHPPPPEKRTGKKVAVVGSGPAGLAA
                     AQQLNWAGHSVVVFEKDDKIGGLLRYGIPDYKIEKWVVDRRIAIMEEEGVEFQTNAHV
                     GEDPTTEELKSEFDAVIMAVGALQGRDLDVPGRELGGIHLAMDYLTQQNRRVAGLPTN
                     AEVEISAKGKNVVILGGGDTSADCLGNSHREGCASVKVLTHGPRPPENPDHLTWPDWP
                     FILHTYPVHEEGGERGWSVAVKGFSGSNGHVERMHAVRTERENGKTIPIEGTEFEIET
                     DLVLLAIGFTGPVRDRLQEELDLGYTDRGAIHSENGYATREPGVFFAGDAKRGASLIV
                     WAIAEGRKAARQADEHLMGYSLLLG"
     gene            28829..29083
                     /locus_tag="VFJ72_11350"
     CDS             28829..29083
                     /locus_tag="VFJ72_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480099.1"
                     /translation="MARIPIWQDDTPDTPPEITEAIEYAKGGTGFALNIQREMANNPK
                     ILKGFSDLVATFYGEEGGAITAAERELAYTTATVVNNCHY"
     gene            29114..29446
                     /locus_tag="VFJ72_11355"
     CDS             29114..29446
                     /locus_tag="VFJ72_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480100.1"
                     /translation="MGLTTEQLNHLGDDPLPEGVYSESEAAIVRYAQVSTSSIFINDE
                     IYDELARHYTDEQIVEICIVVGLSNVVNRFHATFLTDVDEDTTKANAESDAHLGICTL
                     PRPSAVQV"
     regulatory      29544..29657
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            29723..30877
                     /locus_tag="VFJ72_11360"
     CDS             29723..30877
                     /locus_tag="VFJ72_11360"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HET7480101.1"
                     /translation="MGGNHHHHHRRVGPPDPEALKRLRGYEAREREAALGSARWDAEV
                     ERSLEIGLPGADSIEDKTISTFSRTELPHFAGINTFMSFPYLEDVREVGNYDVAILGA
                     PFDMGTTYRSGTRFGPQAIRRISALYSTYNYEFAVDLRESLKVADLGDVFVIPANIEK
                     TFDQIANAVSHVVESGVFPVILGGDHSIGYPDVRGIAPHIDGNIGIIHLDRHVDIQEK
                     DMDERMHTTPWFHATNIPNAPPKNLVQCGIGGWQVPRSGVTVARERQTTIMTIGDVEA
                     MGIEKAAEMALEVAWDGAEAVYLSFDIDSVDGGLVPGTGWPEPGGYLPREALKFLHLV
                     AKEGLCGMEVVEVSPPYDTSDTTALLAARAVADVLATLVTEGHLGTYPAV"
     gene            30891..32381
                     /locus_tag="VFJ72_11365"
     CDS             30891..32381
                     /locus_tag="VFJ72_11365"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET7480102.1"
                     /translation="MIVFGVAFFIFLQVAIGLQFVRRVRGSSTNFIVAGRGLILPFAA
                     AALMAQAVDSNATLGNTDLSAQFGFWAGASLPIGLALCLFLTGLFFAKPMNRMGLLTL
                     PDFYRRKYGRTVEMLAAPIMVLSFCILLAGNLVAGGYLFQAFLGTSFLVGVLIIAAVM
                     LLYTLPGGLLSVVYTDLLQATLAFLGAVALLVFVAMNYGISIPDGMGPFDFEQLTDPK
                     VGAYINWATLIALGLGDIVALDFMERVFAAESPRTARRACYVGALGTLIIGIPFSIVA
                     LSAGSIFKELGVKPDGPVLFALLQDTAPAVIVVIVLSGVVAASLSTGTGAILAMSSVC
                     ARNILGIRPGEGAGRDRLLLITRIGMIPILLFAVIIALRVPQTGILLTLAFDVTLAGL
                     LVPFALGIYWSKSNAPAALVAIVAGSVTRLVLFVLTPTTYGVENTLLYIPNKIFSASF
                     DGIPTILSALVGLAAFVGVALATQKSHEPVTLDLHSREPEPAVAEG"
     gene            32527..34203
                     /locus_tag="VFJ72_11370"
     CDS             32527..34203
                     /locus_tag="VFJ72_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480103.1"
                     /translation="MIGEQERQGSGVKRKFNSFVDNPVLEDYSLRYAPRSFRKWSEYA
                     CATAALGGIAYLVDFAIGASITVSFGFTSATLAILLAAVTIFLTGIPIAYYSARYNID
                     MDLLTRGAGFGYLGSTITSLIYASFTFIFFALEASVMSQALNLLFGLPLALAYVICAL
                     AIIPFVVFGMTLLSKLQVWTQPIWFALILAPFVAILFIEPGVYSDWTRFAGTENHTAG
                     FALLGFGLAAGVALSLIAQIGEQVDYLRFMPEKTPENSFRWWTAVLAAGPGWVILGAL
                     KQMGGAILAVFIISEAGPDGAVEPIRQFVRGFELIIPNGTAALAVATVLVVLSQVKIN
                     VTNAYSGSLSWSNFFSRVLHWHPGRVWWVFFNVGISLALMLGGVFGFLNFVLGFYSNV
                     AIAWIGAVVADLVINKPFLKVSPSYIEFKRAHLYQVNPVGFGAMIAGAAVSIPAFFGV
                     FGEYAQAFSPYLALTIAFVLSPVLALATRGKYYIAREGSTSARLESGDDRYALTTCSQ
                     CGQGYETPDMAHCTFHEEQICSLCCTLEKTCHDHCKKPLEIGRKPGAEPA"
     gene            34410..34712
                     /locus_tag="VFJ72_11375"
     CDS             34410..34712
                     /locus_tag="VFJ72_11375"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HET7480104.1"
                     /translation="MKLSPMEQEKLMIFTAAEVARRRRERGLKLNYPEAVAIISAELL
                     EGARDGQSVAQLMSFGATILSRDDVMEGVPEMVDEVQIEATFPDGTKLVTIHDPIV"
     gene            34723..35163
                     /gene="cynS"
                     /locus_tag="VFJ72_11380"
     CDS             34723..35163
                     /gene="cynS"
                     /locus_tag="VFJ72_11380"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:NF002773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HET7480105.1"
                     /translation="MNREEATQLILEAKREKSLTFEAIAKKVGRHKVWTTAALMGQHP
                     MSAGEADAVVSLLGLEPEVVAVLQEVPMRGSLSSTVPTDPTIYRIHEITQVYGTTIKA
                     LIHEEFGDGIMSAINFNMDIERVENPEGDRVRITYEGKFLPYQW"
     gene            35204..35539
                     /locus_tag="VFJ72_11385"
     CDS             35204..35539
                     /locus_tag="VFJ72_11385"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009682.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HET7480106.1"
                     /translation="MELNAGRETNAITVANTGDRPIQVGSHFHFFETNRALKFDRESA
                     YGMRLNIPAGTSVRFEPGDEREVELVAIGGRRVVRGLNGLVEGALDDADVRDAAFRRA
                     REQGFMEEA"
     gene            35536..37242
                     /gene="ureC"
                     /locus_tag="VFJ72_11390"
     CDS             35536..37242
                     /gene="ureC"
                     /locus_tag="VFJ72_11390"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET7480107.1"
                     /translation="MSDLSRRRYGGMFGPTVGDRLRLADTSLVVEVEQDLTLAGEEAT
                     FGGGKSVREGMAQGAATREEGVMDLVITNALILDHWGIVKADVGVRDGRIAAIGKAGN
                     PDTMDGVTVEIGASTEIIAGEGKLLTAGGIDTHIHFISPQQTTHAIASGVTTMIGGGT
                     GPATGTNATTCTPGAWNIARMLQAVEDLPLNFGFLGKGNSSNPEALREQVLAGAAGLK
                     LHEDWGTTPQAIRTCLTVADDLDVQVAIHTDTLNESGFVEDTTAAFEGRTIHTYHTEG
                     AGGGHAPDIIKLCGEANVLPSSTNPTRPYTVNTVDEHLDMLMVCHHLSRGVPEDVSFA
                     ESRIRAETIAAEDVLHDLGALSMMSSDSQAMGRVGEVWIRTLQTASKMKTQRGPLPED
                     TDRNDNHRVKRYLAKCTINPAITHGVADHVGSIETGKLADLILWSPAFFGVKPEIVLK
                     GGFIAYAQMGDPNASIPTPGPVFQRPMFGAFGSAPAATSLTFVSGYAAENGLRESLGL
                     KKQLTPVRNTRALGKTDLIHNDLLPDIEVDPETYEVRVDGEVITCEPAAELPLAQRYF
                     LF"
     gene            37266..37919
                     /locus_tag="VFJ72_11395"
     CDS             37266..37919
                     /locus_tag="VFJ72_11395"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HET7480108.1"
                     /translation="MLQVSDTAFPTGSFSHSMAVEAFAEAGELQHAEDLKRVAGLHLS
                     TMATSDCVALRAAFGAELEELFRIDRLLTATRLTRELRAASSSTGRRFLLSVAALGVE
                     GAVLGAFTDAARNSETPGNLAVGYGVAAPALGLTLDETLRAYLYSTAASLVAAGQKLI
                     PLGGSAAQRVLYELGDKIEIAAKTSETMEVEEMYAFAPTIDVRSMMHERQRTRLYIS"
     gene            37916..38590
                     /gene="ureG"
                     /locus_tag="VFJ72_11400"
     CDS             37916..38590
                     /gene="ureG"
                     /locus_tag="VFJ72_11400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET7480109.1"
                     /translation="MSARELALRIGIGGPVGSGKTALVAELCRRLGDELAIAAVTNDI
                     YTREDAEFLQRAAVLPEGRVVGVETGGCPHTAIRDDVSMNLEAVEALERKFSLDLIFV
                     ESGGDNLAASFSPELVDASIYVIDVAGGDKVPRKGGPGVTRSELLIINKIDLAPLVGA
                     DLSVMDRDAGALRGEAPTLFASVLHGDGMDAVVGWVREAVLDRRWVPATSTARRDHHH
                     EHPHSH"
     gene            38778..>39375
                     /locus_tag="VFJ72_11405"
     CDS             38778..>39375
                     /locus_tag="VFJ72_11405"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET7480110.1"
                     /translation="MDVSLAPGSSATVLTQAANKAYRGTRASQQTVLRAGEGALLEYL
                     PHHLIPYSGSSYSQRTIFHLAPGAALITWDAYSAGRIARGERFAFDSLHGRTAIFRDA
                     VPEAIDGFELIAGGEPFGGYAYMAAAYISKPEPLGALADELHASLGGMPGTLASASAP
                     SPHLCVVRVLTHSARYLYRLLNLTRHTAREFLKLPAPAR"
BASE COUNT         7517 a        11893 c        13280 g         6685 t
ORIGIN      
        1 gctgccgatg ataatagtcg tctacaacga cgccgcctac ggcgcggagg tccaccactt
       61 cggtccgatg ggccgtccgg tcgacctcac gcagttcccc gataccgact tcgccgatct
      121 cgcccgcgcc gccggagccg aaggtatcac ggtccgcaca ccagaagacc tcgcgccgct
      181 gaaggagtgg ctcgggcggc gggacgggcc gttcgtcata gacgccaaag taaaccccgg
      241 cgtgcgggcc gagtggctcg aagaagcgtt ccgcgcccac tgacaggagg agagaagaat
      301 gtccgagaac cccgtagcat ccttgataga agcactctcc ggcggctcag tcgagatcgt
      361 ggacctcacg cagccgttga acgagaagac gcccgttatt cagttgccgg agcctttcgt
      421 caacacgccg gggttgaagg cgcacgagat cagtgcctac gacgaccgcg gtccggcgtg
      481 ggcgtggagg tggctggaga tcggggagca cgtcggcacc cacttcgacg ccccgactca
      541 ctggatctcc ggccgcgacg gcctcgacgt ctcccaggtc ccgccccgac atctcgtcgc
      601 ccccgccgtc gttatagaca agaccgccga ggtcgagaaa gaccccgact acctcctcac
      661 cgtcgaggac atccacgcct tcgaggacga gcatggcccg ctcccagacg gcggctggat
      721 cctcttccgc accggctggg acgcccgcgc ccaggacgag gaggcttttc tcaacgcctc
      781 cgccggccag ccgcgttcgc ccggcttcga cgccgagtgc gcccgctggc tcgccgagga
      841 gtcgccgctc gtcggggttg gtgtggagac cgtcggcata gacgccggcg cggcgggcgg
      901 cttcgatccc ccgttccccg ttcactacta tctgctcggc gccggcaagt acggcgtaac
      961 gcagctagca aacctgggtt cgctaccgtc gaccggcacg ctcttgatga tcgcgccgct
     1021 caagctcacc ggcgggaccg ggagtcccgt gcgtgcgctg gcgctcgtgc cgcggagtta
     1081 gcgcgtagcg ttgagtggtt agatcaaacc ggaacacttt cgatgatgtc gtatggagat
     1141 ccggtcgccg tgatgttacg caactcgaat tcggcctggg ctagcagact gcggaactcg
     1201 gcctcggttc gctctttacc gccgggcatc accagcatca tgaggtccat gaatttcatg
     1261 gggtcgggct cgttcggcgg tccgaagacg cgctcgacaa gtagcagcct accatcgtcc
     1321 ggtatggcct cgcggcactt gcgaagaatc ccggccgctg tctcatcatc ccagttgtgg
     1381 atgacactct tgaacaggta ggcgtctgct ccccctggca ctgcctcgaa gaagctcccg
     1441 ccgacaactt cgcagcgtcc ggcaacaccc atgcgctcca gcaggtcttt tgcgccggcg
     1501 acgacgtggg gctggtcgaa gagaatgccg agcaggtcgg ggttcgccgc caggatcttt
     1561 gcgaggatct cgccctggcc gcctcccaca tcggcaagca cgccgatccc cgagaagtca
     1621 tagctgtccg cgatcgcctc ggctgcgccg gccgagaggt gtgtcatcgc ggcgtcgaag
     1681 atcccgctct cctcgggatg tgatgtccgg tattcccaga cactcattcc gtataacatg
     1741 gggaaggctg tctcaccggt cttagcgctg tctataaggt gagcccaggc cgaccaggtg
     1801 tagggccggc cgatgaacgt ggcccaggcg cgtatcgagc cagggacatc ggatcggagg
     1861 tactccgcca gcggcgtcag gccgaagcag ccttccttct cgacgaacac gccgacgctt
     1921 gcaagagcgc gaagcagccg gtatagggtg ggcgcgtgag tcccggtggc ctcggccagg
     1981 tcatctacgc ttctgggact atccttcagc aggtcggcga ttccgagggt tgctgctacg
     2041 tggatagcct gggagacctg gtaaccgttc accatccgcc atagagagtc tcggggagag
     2101 acctcgctcg catccatcag ctttgctcct ttactaacaa tgagagacca gcatagatcg
     2161 cccggctgat cgggtcaatc agccaaacat gaagtggcct tctctcaggt agattggtag
     2221 aggtagttgc tgaagtggcg tatattccga gaagtgtaag agcttgcgat ccagcgacgt
     2281 agcctgaagt attaagagag caactcctcg aagctggctt cgtccagcac cggtatgcca
     2341 agctccctgg ccttctccag cttggagcca gcctcctcac cagctagcac gtagtcggta
     2401 ttcttgctga cggaggaagt gaacgtgcca cccgccgcct cgacccgctc gacgtagtag
     2461 ctgcgcggct ggctgagggt gccggttatg acgacccgct tgcctgctag cggtccttcg
     2521 gagggacgtc ctgactcgcg ctcgaagtcg aggccgagcc gcatcaggcg ctcgaccaat
     2581 tcctggttgt cttcgaggtc gaagtattcg accacagcgc gggctacaac ggccccgatg
     2641 ccgtctatct cgacgagttg ctctaccaag acgccccgca ggaggtcttc gccggagaac
     2701 ctttcggcga ttagctgcgc cgttacgctc ccgacgtgcc ggatgccgag ggcgtagagg
     2761 acgcgggaga agggctgttg cttgctgttg tgtatcgacc ggatcaggtt ctccgcgctc
     2821 ttctcgccga agccttcgag cggcaccaac tgatccacct caagctcgta gatgtccgcg
     2881 gcgtctttta tgaggccgag gtcgaagagg cgggtggcga gcttctcccc taggccgtct
     2941 atgtccatcg cggacttctg cgcccagtgg atgatgtgct ccaacgcctg cgccgggcag
     3001 cgggcgttca cgcagcgcgt tacggcctcg ccctccgggc gggataccgg ctccccgcag
     3061 acgggacagt aagtgggcat cacgaactcg cgttcggcgc cggtgcgttc ctccgcgatc
     3121 ggctttatca cctgcgggat aacgtctcct gcccgttcga tcaccacctt gtccccgatc
     3181 aggatgcctt tctcttttat gtagtcctcg ttgtggagcg tggcgcgcga gatggtcacg
     3241 ccacccacgt ttaccggctc catgacggcc tgcggtgtca gggcccccgt gcgcccgacg
     3301 ttaacgatga tgtcgttgag cttcgtgcgg cccgcgagcg gctcaaactt gtaggctatg
     3361 gcccagcggg gcgccttcgc gaccgagccc agggcgcgct gctgatccct tgagtccacc
     3421 ttcacgacca cgccgtcgat ctggtacggg agcgactcgc gctccctcgc ggccttctcg
     3481 cactcctcga tcacggactc tatgtcctcg tgcagcctgt aagggttcgc gcgcaggccg
     3541 taggacttca gagcgtccag agcggcggag tggctctcgt agaggtcgcc accctccccg
     3601 atgccgtaga ggtagacggt gagcggcctg cgggccgtta tcttcgagtc tagctgccgg
     3661 atggagcccg cagcggcgtt gcgcgggttg gcgaagggcg gcttcccttc ctcctcctga
     3721 cggcggttga gcttctcgaa ctccgccagc gtcatgtacg cctcgccccg cggttccagg
     3781 gtctgcggcg gatcgtcgtc gagcttattg gggatggagc ggacggtgcg aaggttcgcc
     3841 gttacttcct cgccgactgt gccgttgccg cgcgtcgcgc cgcgggcgag tcgcccgttc
     3901 tcgtagcgca gcgagatcgc cagcccatct atcttccgct ccgtcacgta acgcggcgtc
     3961 tcgtcctcgc ccagcaggcg ccgcacgcgg gcgtcccact cccgcaggtc ctcgaccttg
     4021 cgggcatttg ccagggagag cattgggacg gcgtgccgga cctgctcgaa ctgctccagc
     4081 gggtcgccgc cgacacgctg ggtcggagag tcgggggtga caagctccgg gtttgcggat
     4141 tccaggcttt cgagctcgct gtagagggcg tcgtactcgg cgtcggggat ctccggcgcg
     4201 tcctcgatgt agtagcggcg gttgtggtag cggacctgct cccttagctc ctcgatcctg
     4261 cctcggatgt ccgtcttctc tgccaccgtg cggattatac gcgggacgat ccacgtcccc
     4321 aagagccgcc ttcagagaga ttttcgtata ctgaacggag tatgggagga cgtccgaaac
     4381 acaggcaact ccagtcgcga gaggacttcg agcgggctct tcaggaggag atctccgtct
     4441 ccaaccagtt cgatctgccg ctctgcgtgc tggtcgcggt gatggagggc ggctgggacc
     4501 cggaatccga aggccgggca gtcggcgccc tgcgcctggc ggacctcgtc tcgcgcaccg
     4561 atggcccgga cctgatcgtc gtactctcga acaccgctct ggagaacgcc cgcgccgtcg
     4621 aacggcgcct gcggcagata ctgccggagg tcgggatcgg gatctcggag ctcacgccag
     4681 gcgatacggc gcagcggttg gtcgaacgcg cccgccaggc ggctttacgg aacctctaac
     4741 cgcccgctcg ctccaacggc cagcctagct tcctgccacc ctgatctaca aagacgaact
     4801 ccgcgtccgc cggcgcgccg cgcaccacgg ccacgacata cggatacgtc aggacctgcg
     4861 ccacgaaagc gtccttcggc ggcctcttca gcgcgagccc gaccatcacc tttccgccct
     4921 cgcctcgcgc cgatctcgcg gccatcgaga acccgcccgt cggcttctgg ccccagaaag
     4981 cggccagata aataccctcg ccggagtccg gtatcttcgc cttcggtccc agcgccttcg
     5041 agagcgcgtc cgccgaggag gcgaccacga ccttcggccg cttcggaccc tgacccggag
     5101 cgttcgccgc tatctgttcg agagagatct catttcgttc catcgaggac tccgtggtct
     5161 cctgagtctg cgtggtggaa ggcgccgtgc tctcctggga agtcgtgttc gagccttccg
     5221 gggacgagcc tgagccgccg cccgccgcgc atccggcggc gaggagtatc aggagcagga
     5281 tcgtgccggc tcgcagcatc atgggcatct tacatcaccg atttgggggc acgccgatca
     5341 gacgacgccg gaactctgac ggctgccgcc ggcttcgagt tccgtaaaga gccggtctat
     5401 ctcgtcaccg actatctcct cgcgctccag cagggcatcg cgcacgccct cgacgatggg
     5461 ggccttctct cgcagcagtt cctcgatctc ccgccgcgcc tcggcgagga tggagttcga
     5521 ttcttcgcgc acgccggagt ctcgcagcag ggcgccgatg gaggagccgg ccatctcgtt
     5581 cgggagcgtg ccgaacgagt acaggccctt gcccatcccg aacatgccca ccatcgtcgc
     5641 cgccgcccac gtcgcctgcc gcaggtcggc gctcacgccg gtcgtcgtct ccccgaagta
     5701 gatgttctcc gccgccccgg ccgcgagagc cgtctttatg cgggctataa cgtccgaacg
     5761 ggtgcggacg tagcgctcct cccgctcctg gtagctaacc aggcccaggg cgttctcgcg
     5821 cttgatgatg gtgatcacct gcatctggag ctcctcgcgc tccaggtacc aagaggcgac
     5881 ggcgtgcccg gcctcgtgag tggcgatctt gaccttctcc tcctcgttgt actcgacggg
     5941 ctgcttcagg ccgatctcat ccgtcagctt cgccgcccag atgtcctccc agcccagcga
     6001 gtctcgcccg tcctgcagcg cgaagatgag ggcctcgtta atgatgttct tgatgctggc
     6061 cggcgagtac ccgaccgtcg ccttcgccag cttctccagg tccacgtcct cgtggtggac
     6121 cttcttgagg tagtagtccg cgatgtccgc gcggccgttc ttgtccggca ggcccacgtg
     6181 cagccgccgg tcgaaacgtc ccggacgcag gagcgccggg tcgagcgagt cggccttgtt
     6241 cgtcgccccg ataaccagga tgttgtacga ggggatcttc ctcttcaggt tgcggcggat
     6301 gaacagcggg atcaggcgcc ggaaaaaacg ccttattcct ttctgctgct ccatcccgtc
     6361 catctggacc aggagctcgt ttacgagcat gccgcccatt cccatcccac ccatgcccat
     6421 cccgcccatt acccggtcca cgaagccgga gcgtttctga gcgtccgccg gtcccgcgag
     6481 tagccggtcc tccaagccgt ggcggaacgg ttcgtggaag gggtatggct ccatcgtctg
     6541 ggcgacgccg ccgcgcgagc cgatggcgtc taactcgtcc atgaagatca ccgctccacc
     6601 gtaccgctcg ctgaacttgc gggccttcgt gaacagccgc atgaccttga ggttgccgat
     6661 ccccatgaac atgctgctga aactcgaagc cgaggcgtag atgaacggca cgttcacctc
     6721 gcccgctatg gctttagaga gcaacgtctt gccggttccc ggaggacctt ccagcaggat
     6781 gccctgcggt ggatagccgc ccatcttgcg aaacttcttg aagccctgga agagccggac
     6841 gacctcacgg gttgcctcca cggccgcctt ctggccgcgc acgtcgtcga aggtcgcatc
     6901 gtactcgccg gggtagatga cgtaggtgcg gccgcgcgcc aggaaccaga acagcgcgac
     6961 gaactgcacg atcaggaaca tcatcgcgaa gaacagcgtg aacccgagcc cgaacagctt
     7021 caccagcagg gccggtataa agctattcgc cgcggctacg gagtacggcg gcgctccgcc
     7081 ctggccccac gggaagatca tccagatgct gagtaggtac agcgcgtaga gcaagaggac
     7141 gagcaagatg cgccgggtcc agcgtttgat cgagtttatg ccgacgacgt gccgctctcg
     7201 gaagctttcg gcgtaagggt ctctatccct gcggcgaacc atgtctagtt cccttcttcg
     7261 aatctatgta ttcagtctgc tctcgccggt agcaagtttc aacccttcga gcagataacg
     7321 gcctctctca tgcattacac tacgttgccg atgcgctcct ggatctggaa aatcctcatt
     7381 atctccatcg ttgtcgcctg cgccctggca ccggtggcgt gcggcgcgaa ccagaacact
     7441 tcctccagag atccatccgg caaggaggga tctaccctgg cgacgacagt cccggaagac
     7501 tcagccggag ttcaggaagg agccggagct gtcgccgccc tttcgggagt ccagaaggca
     7561 gatcaggccg cagcagagtg gcagagtgac gcggaactct acgccatcgc cagcctcaag
     7621 ccgcaggtgg acgcggaggg ccgcgcgccc gcctggctct acacttacgt ctcgtcttcc
     7681 aggaaggcgg tggtgagcgt ctcggtatcg ggcgggaagg tcgcggacac ccccgaacag
     7741 gcgctgcccg acgagcagat agaagatatc tccaaccaca ccctcccgcc gcccgcgcgg
     7801 ttgctcgaca gcaccgaggc cgtgaagaag tccgggaagt ttctcgatac cctcaagagc
     7861 gagccggggc tcgacgcttc cgccggcctc gactccttct ccaccggaaa gccggtctgg
     7921 atcttctcta ccgccaaagg agataagcgc gtagaagagc gagtctcggc caccggctcc
     7981 tgagagtcag acctcgctct cgccgcgcga gagcgaacct aaccgggctc ggagtagtgc
     8041 tcgcggagcc tctcgtcgaa ggcgccggac gggtcttcga gggtgggagc ggactctatg
     8101 gtctcccgct cataaggggc gtggacgcgc tcccggtcgg ggtcgacctc gatctcgtcg
     8161 gccggaatag tgtgtccgcc ggagaccacg tacttcagga cgtcggtcgg ggcatcgtag
     8221 acgatgtcgt ccaccctgcc gaactcttct cccgaggcgt cgaggagcgt gtagcccagc
     8281 agcgcggtgt atgggttgtg cggttcggga ccagatctct ccatgctcca acctccggct
     8341 ggcgtcaatg cctgaatatg cgtatgatac tccgaacgtg aacgccaaaa ctaatcaccg
     8401 gcgcaacgcg cgcaccctca agacgctctc gctcctgtac ctgggactga cggtggtctc
     8461 gctgctgttc gtgcacagcg actcctacga accggcggtt tcctatgtgc tgctgtactt
     8521 cgcggtctca atcggcgtgg tgacggcagt ggctctgctg ctcttgccga ctcagcagta
     8581 cacgggaggg ttctcgaccg gagcctacgc gctctacgcg gcattcgtcg cgatgatggt
     8641 cttcttctcc ggcggagtat cgagcgagct gtacgtgttg tacttcccgc tgcttctcgc
     8701 ctcggcgctg cacggccact ggcggatggt gatgatggca gtgatcttca ccctgttcac
     8761 ctatttcctg gcggcgacgc cggacatcat gaacgaggga gccgcgacgg gcgccgccgc
     8821 gctagccttc ttccgtctcg ccgtcttcgc cctgacaggc gtcttcctgg tcgccgccgc
     8881 gcggaacttc gccggagccg ccgactccga ggactacgac tacgacaccg acgaggacgg
     8941 ctcgatgctg cgggaacggg tcgaggcgga gttggcggcg cgcaagggtg tgccggtcgc
     9001 ggtcgtgctc gtggaccccg gtcggaaagt cgaagacgtg gaactcctgt tggagcgggt
     9061 gcggggtagg atcggggatc cggtcctgct aggggagggg acgtgctttg ggatcgtgct
     9121 ctccggcatg gacgaccggg gtgtggagag cgccgcgaga cgcgccctgg cggcggcgag
     9181 ctcgctcggc gccgaggaga cacgggccgg agcggcgatc tacccgcgcg acgcccgctc
     9241 ggcgaacgac ctcctcgtgt ccgccggaca ggcccttgaa gcggctttcg agatagagtc
     9301 accaagcgcc atagtcctcg ccggccgtgg cacgccccgc gccgagtctc gctaccgggc
     9361 tgcccgttag agccttcgcg aagctccgct cgcagcggtc tctgaaacct cttcggggtc
     9421 cgaacgtacc gatggtataa tctgcgacgt attctgaagg cgtgttcacg gccggggggt
     9481 agcggctcat atgggaagat ttttcagggc gatacgggga tttttcggga gttttctctc
     9541 cggggtagag cagcgcaacc cggagattct gctggagaac gccgttcaag acgagttgga
     9601 gaacctgaag aagctccagg tcgccgccgc ccgcactatg gcctacgaga agatgctcgc
     9661 caacgacggc gcctcgaagc agaagatcat cgccgccaaa gagcgccagg cccgtgagct
     9721 tgccgcccgc ggccagcgcg acgccgcaat agaggtcgtg cagcagaagc aggaagccca
     9781 gacccagctt gcggatatcg agtcgcggct ggaagaggcg cgcaataact cccaggagat
     9841 ggagcaggca ttccgggacc aggagcgccg ctacaacgac gtggtgcgcg agcgcgccgc
     9901 cctgatggag gagcaccagc gctcacgcgc cctccgcacc gccaacgagg cccgcgccag
     9961 catctccctc tcggactcgt cgcgcgacct ggacaaggcc cggcaggcta tcaggcagtc
    10021 ctcctacgag gcccaggccg tcggagagct cggcacgagc gagaccgagc gccagatcgc
    10081 cgccgccgag gtggatataa agcgcctcga cgccgagcgg gagcttgaga tgatggagct
    10141 tcagatgggc ctgcgcgacg ccgactccct cgaagccccg gccaatgaag agacaatcgt
    10201 tgagcagccg gtggagggcg tcggcgaggc ggagccagcg gagccggagg agcgcccgcg
    10261 tgggtagcct ggaggtccga cggcggcagg gactgagcat cagcgcggcg cgtcttattc
    10321 ccgcgggatc cttcgcaggt ttggctggac tcgcggcgtt ggagacacat tacttcctcg
    10381 ccgggttgct cggagttggg gcgttcgcat tgggccggaa gctcctcaat cgtcaggaga
    10441 accggcgcag ggagcttcgt cgacgggcgg tttcgaacgt gcgggagctc ggccgggtgg
    10501 cgcgcgagga tcgggtggcg gcgtcgcaga tgaaacggtt gatggccctg cagaacgggc
    10561 tgctggaaag ttgggagctg ttgccggagg agtaccggcc gctcctcgac gacgatatct
    10621 tcaacatagt cggcgaggta gagggcacgg cgcggctggc gcggcggcgg acggcgctgc
    10681 ggaagcatct gcagagcctg gaccggcgcg ggatcgagcg ccggataaag agtctggaga
    10741 gagacctcgc cgggttggag ccgggctcgc cgttgcgggc gccgttcgag agcgccctct
    10801 ccggcaggcg tgatgagttg gccggatacg gggagcttat ggatgggatc agcatgataa
    10861 acgcccagtt agagggagcc gagagcctgc tcggcgggct gcgcggggag cttctggcgc
    10921 tggacacgag cctcgcgccg ggctctctcg aatcggggct cgtgcacctc aaggagcggg
    10981 tcgcgtactt caggcgcggt ctggacgagg ttacgcggag cgtcgagacg ttgcccgaga
    11041 ccacagtgga ggtgagtgtc tgatgagcga agaccgttcg aggcagagct tcttctcccg
    11101 gatgttcgcg cccgtcgacg acgaggggac gacgacctac tcccgagacg acattggcga
    11161 ggagcatttc gagcagccgg agggggagag cgtggacgag cctcgtggct tcaccgtgga
    11221 gcgcgccgcg gacatcataa gagagctgcc gccggaggtt ccgcgccgga gcgccgtgcg
    11281 gatagtgcgc caaacgctca ttgccgccgg tatcagcgtt gacgacctcg gtcgctccac
    11341 acgggtgcgg gaatccaagc tcaactccga gatagagctt cgccagcagc gcatagaaga
    11401 gttgcagcag aacacggacg aggtcatacg ggacctggaa gaccagatca agaaggcccg
    11461 cgaggcccgc gacttcggcg tcgccgagga ggagcgcgga atctccgagg cccgcgaggg
    11521 gctggaggac gtggagaagg tccgggactt cttcgacctg ccgcgcggcg agacccgctc
    11581 gaccccgttc ggagatcagt ccggcgaggc gaccaccgga gagaacgcca cgtatcctgt
    11641 gggagacgag acgcaggtca tggaccgctc cgaaatggac cagccggaag atccggcgaa
    11701 catggacgac acccagatac tgcgcccgcg cggtccgctc tccgaggagt gggaggcccg
    11761 aagggatacg gagaaccgcc agccgtgagc atcccgcgcc gcctgggacg catggcgcgc
    11821 ggcttcgtct ccaatatcca ggaggacgag cgtctgcgcg agagcctgcg agagacaatc
    11881 cgcaccggcc gggagcgcag cgagaacctg agagaggcat tcggcgcggc gtggagggga
    11941 gccgccgagg agtggaggaa cgccgaagaa cgccgcgcgg cagaagagga agtcggagag
    12001 cggatcgccg ggcaggaacg ctcggagcgg cgcttctcac cggggacgtt cgtgccgagg
    12061 atctatcctc tcaacgtcct ctccgcctat aaccggctcg gtctcgaacc cggcgcccct
    12121 ctggaagaag ttagccgcaa gcgccgcgag ctgatcaaga agtaccatcc cgaccgcttc
    12181 accgatccag acaagcgtat ccgcgccgag cggctgtcgg ccgagatcaa cgccgcccac
    12241 gacaccatcg agcgccacat gagcaagagc tagccgctcc gccggttaac cgctgacgag
    12301 ctctccccgt actatgagac ggttctcgaa ggtcgggatt ggggtgcagg tctggagcga
    12361 tatggtggtc ttaccggcta cgggataggt tacccagtag tcggcgggac ggacggtcat
    12421 gaggtcgtag acgcggtaga cgtactcctt tccggtggtg tctttgactg agatctcgtc
    12481 tccagggcgc atcttgtcga gcttgtagaa gacgtatggg accctggtct gcatgaaccc
    12541 cagcgcatgg ccgacgataa aggtgttcga gcctctctgc caggggacgc cagtgttctg
    12601 aaggtggatt atcccctccc ggtccagcac ggcctggctc gcggccgtag gtatgtcgag
    12661 gtcttttatg ccgagcttcg gtatggtgag cgtcatcgcc tgcgccgctc cggtggcggc
    12721 cttctccggc gttttcggcg ggctctcggg cggttcggcg agcggagcgg cgttctctcc
    12781 gaggagcttg ccggcagtct ggacctccgc tgttcccagg tccaccgagg acgactccgc
    12841 ctgcgggata atgggaagcg ccagcagcgc gaacagcacc acgggcacga gtaggaccgg
    12901 cgctctaaga cttccaaaca cccacgaaag tatacaggag cccctgaaat gccggtagaa
    12961 taagagcatg aagatagagc ccctgatcga ggagttcggc gctcacctgg ccgatgacga
    13021 gcacaccagc ccgcgcaccg tcgagttcta cgaggcggac ctccgcgagt tcgcccgctt
    13081 cctcgccggg aacgatatca gggagatcga gagcgtcggt gtcggcagcg tgatggagtt
    13141 ccgcaaccac ctgctcgaag ggaagcgcaa gcgcttcacc gtctaccgca aggacgccgc
    13201 cgtgcgccgc ttcctcgact gggtcaggac cgcgaccgac gcggacctcg atttcgaccc
    13261 catagagccg atgcaccagc cgctggacca gcagatagtc tttctcgaaa acgaggaagc
    13321 cgagcagctt ctatcttttc ccacgcagga gcgcgacgcc gctcgcgatg ccgccgtcat
    13381 acgactcatg ttagatacgg gtgtgacggt cagcgagatc cggggtgtgc tcaggagcga
    13441 tgtggacctc gacgccgcgc agataaatct cggtggaccc ggctcgcacc tggcgcctcg
    13501 caccacccgg ctctccgaca ccaccgtcga agccgtccgg gagtatctgg agatgcgcgc
    13561 ggatgagacg ccggagctga tagtgggccg ggcggcgcgt cccgtgacga cgtcgaagtc
    13621 tcttcagaac gcgatctgga agcgctgcga gcaggtcggg atgtggcgcg tctccccgat
    13681 gaatctccgc cacaccttcg ccataaggct cttgcggaga ggagccaccc ttaacgagct
    13741 caaggacgct ctcggcgttc gcgacacgac caacataggt gtctacaaga agttcgttta
    13801 gacaacaagc tccagatatc ctgtaactct cctgcctcga tcttcgatgc ttcatatcgt
    13861 aacgaaacag gaccggccag ggtccaacta agaaaagcaa agtaacacga agcagaacgc
    13921 tgattagatc acatcaggag tttgcaccta atcggggtgt ggaataaagg atgtgggatt
    13981 caagaaacac aagttataaa aggctcggcg gacgaaggga tcagcaaggt ggcatccgaa
    14041 agttcacaac aatcatcaaa tacccagcaa ccgaaagata ccagctcgca gaagaaccaa
    14101 tcagccgaga cgcagaacaa ccaacaaaac caacagaacc aacagaagaa ccagaacgct
    14161 cagcagcagg gctggcagcc gtccgacgac gatgaggacg ttcgctatca gcagaacctg
    14221 accgagcagg agaaggcgga gatgagccag gagttgtcgg agatagacga caacgctccg
    14281 gttcgcaaga acgatcctcg cacctctgtg cagaagaaag aggagtaccg gttcgcggcc
    14341 aacatacccg gcgacaagac gatcccggag aaagagtacg cggactatac gctggagtac
    14401 gacgaagatg acgccgccat gaacggcaag atcccggacg tactgctgga tgtgccggtt
    14461 gtcaagatag acgagatcaa cttcgagctc aacgacctgc gggcgaaggt taacctcttc
    14521 gccaaggtgc tcgacctggt ggaactctcg gtcggggtag acgcctacct gggcagggtt
    14581 aagctcgtta taaaaggagt cgaagctcag gcgctcctga aggtccggct ggataacgtg
    14641 acggccatac tcgaccgggt tctcactacc atagaccgca accctcagat catcgagaag
    14701 ctggtagagg gcgtaagctc tgcggtctcg gacattggcg ctggtgccgg agacctggtg
    14761 ggcgaggggc tcaactcggc cgtcgaagac attggcgcgg gcgctgggga cctggtaggt
    14821 gaaggactca actcggccgt cgaagatata ggttccggcg cgggcgatct tgtcggcgag
    14881 gggctcaatt ccgccgtcga ggatatcggc gcgggcgcgg gagatctggt cggtgaaggg
    14941 cttaactcgg ccgtcgagga tatcggcgcg ggcgccggct ccgctgtgga agacattggc
    15001 gctggtgccg gagacctggt gggcgaaggg ctcaactcgg ccgtcgaaga cattggcgcg
    15061 ggcgctggct ccgcggtcga ggacataggg tctggcgcgg gatcggcagt ggaagacatc
    15121 gggtctggcg caggccacgc cgtgggagag ataggtgaag gcgctggctc tgcggttgag
    15181 gatataggtt ctggtgctgg tcatgcggtc ggcgagatcg gagagggcgc cggttctgct
    15241 gtggaggata taggctcggg cgcaggccac gcagtcggtg agatcggtga aggcgcggga
    15301 tcggctgtgg aagacatcgg gtctggtacc gggcatgccg tgggagagat aggcgagggc
    15361 gcaggctcgg ctgtagagga tgtcggctcc ggcgcgggac atgcggtcgg cgagatcgga
    15421 gagggtgcgg ggtctgcggt agaagacgtt ggagagggcg ccggctctgc cgtcgctgat
    15481 gtaggcgaag gcgctggtga ggctgttgag agtacaggcc aggctgtcga gagcgtcggc
    15541 gaaggagcgg gttccgcggt cgaagacgta ggcgaaggcg ctggttccgc ggtgaaagac
    15601 gtgggcgagg gcgccggaga agccgtcgag agcaccggtg aagcggtaga ggatgttggc
    15661 tcgggggctg gctccgctgt ggaagatgtt ggtgaaggcg cgggccaggc ggttgagagc
    15721 acgggcgaag ccgtcgagaa cgttggagag ggtgcaggtt ccgcggtaga ggatgtcggc
    15781 ggcggtgccg gtcaggcagt tggagaagtg ggacagggcg ctggcgaagc agtcggggaa
    15841 gttgggcaag gagctggaca ggccgtaagc gaggtcggtg aaggagtcgg agagaccgcc
    15901 cagcaggtcg gtgaaggagt tggacagact gcccaacaag tcggtgaagg cgcgggtcag
    15961 gcaatcggcg aggttggcca gggcgtcggc caggcgactg agcaggtcgg agaaggagcc
    16021 ggacaggctg ttcaggaagt tggagagggc gccggccagg ctgccggaca ggtgggagaa
    16081 ggagccggac aggcagttgg tgaggtcggc cagggtgctg gacaggctac gcagcaagcc
    16141 ggggaggctg ccgggcaggt cgcggaaggc gccggacagg cggcacaggg tgtcggtgac
    16201 gccgcgggtc aggcggctca gggtgtgagc gacaccgccg gccgggcaac cgagcaagtg
    16261 ggtcaggtcg cgcagggggc cacggacgcg gtcgagcaga cggctgaggg agctacggac
    16321 gcggttgaag aggcgaccga taccgcaggt caggtagcgg aaagtgctac ggacgcagct
    16381 cagggggtga cggagcaggc gcaagacacg gtgggacagg caacccagca ggcaacggac
    16441 acggccgggc aggtcgggca gcaagctggg gagaccgtcg aaggggctgt ggatcaggcc
    16501 caggagacgg ctggcggcgc ggtggatcag gcccaggaga cggccggcca agcggtggat
    16561 caagctcaag acgccgcagg acaggccgca gaccaggccg gtcaggctgc agaaggtgcg
    16621 caggaggccg ccaatcaggt cgtggagcgc acggtggatg agtcggggaa catactcgag
    16681 accgtcacca acgagaacgg cgaggtgatc ggtgaagacc tcgtcggtac cgtcggcgac
    16741 ctgcccattg aggaggagta catagatgac cagggccggg tggtgagccg ggcgaaggac
    16801 gactcgggga acgtatacga gcaggtgttt gacgacgagg ggaacgtggt cgaggtgagg
    16861 tccgcgtgag ttagctggag gtcgtgggtt cgttagatag gggaagcgaa gggctcgaag
    16921 aggaggaacg gtggctgagg atcaggtaaa cgaggcgttg gagaaactaa atcgcggtat
    16981 ccaggagtcg aagcagatgg tccagcagca gactatgcaa cttgcgcagg acttctttag
    17041 tgactccgtg gaggagttga agcggcacat aaaggagaac cgctccaccc tggaggggct
    17101 gccaggtcag ataccgggtg gccaggaaga gtcgtttcag atgctcttcc aggaactgat
    17161 ggacaattac caggctatag agcaggctct cgacgaggcg gagaagaacg tctccaacct
    17221 cgacaccgac agcctcgtgc agcagggaga ggtcgaggct acggacgccg cccgccgcga
    17281 ggctagagag cttggagttg acctcacgga gatagagggc accggcacgg acggccggat
    17341 caccgtggac gacgtcaaga aagcctccga ttctgcccgg gaggaagctg gcgaggctgc
    17401 cggaggagcg gctgatgagg cccagaaggc ttcggatgcc gctcgccgcc gcgcagagga
    17461 gatgggcgtg gacctctccc agatagaagg cacgggctcc gggggcctca tcaccgtcaa
    17521 ggacgtaacg agcctggccg aaggcgcggg ggatcaggcc acccaggcgg ccggacaggc
    17581 ggctgatcag gcgcaggaca cagccgagca gacagcggcg caagctgatg gagcggtagg
    17641 gcaggcgcaa ggagccggac aagccgccga tggcggaaac ggctcggaag gtccgaaagc
    17701 taccaacgcc gcgcagcgcc gcgcagagga gatgggcata gacctctcca ccctgaaggg
    17761 ttcgggggca ggcggcctca tcaccgtaaa agacgtggtg aagtcctgac atcgtgaggg
    17821 agaagcgccc ggacgtatcc gagaccccgg acatcgaaat taccgccggc gtgaagaccc
    17881 gcaggctgcg tttcgaaaag gtgccggaga cggaagtgag gttcagggga taccccgacc
    17941 gcgagtcggt gtcggggacg gaccgcgaga acctgccgga cagggtcgag cggggtgtta
    18001 cctaccgtga cgccagggtc cggctacgga tcgccagcga aatcgcggag agcgcattcg
    18061 agacaggaga cagagagccg gggcacggct ccgaagatcc gaagcaatcg gtataaacga
    18121 acgagtagaa ggagagaaac atgaccgaaa acgcgcagaa tctggcccag cagatccagg
    18181 agcaggccgt caacagcgcc aaggatttct acggcaacgc gcttggcgag gtgaagggac
    18241 agttggagag cagccgctcc cagttggagg agctacttga gcagcttccc gaagggcagg
    18301 atgaagcccg ctcccagatt caggagttga tcgactccta cgagtccatt tcgaacgctc
    18361 tggatgaggc ggcccagacc cagggggtcg aggacactgt taaccaggcg gtccagcagg
    18421 cgcaggagac ggccgaagga gctgcccagc aggcgcaaga caccgcgcag ggagccgcgg
    18481 accaggctca ggacgctgcc ggcggcgcgg ttgaccaggc gcagcagaca gccggtcagg
    18541 ccgcgggtca ggcacaggat gccgccggag gagcggtaga ccaggcgcag gaggctgcgg
    18601 gacaggccac cgagcaggcc ggacaggctg cggaaggcgc gcagggcgcg gtcggtcagg
    18661 tgacggatca ggtcgggcag gttgccgaag gagcgcaaga tgccgccgga ggggccgtgg
    18721 agcaggccgg tcaggtcgcg gagaacctgc cgggcggcca gctcctgagc gagacggtca
    18781 atgacgcagg ccagactgtg cagcgggtag tcgacgagtc cggggacatc atcgagacga
    18841 ccctggatga ggcgggaaac ctgatcgatg aggacatagc cggcaaggtc actgacctgc
    18901 aaccggccga gggatacgag gaataccagg acgagcaggg gaacaccata cgcacggtaa
    18961 cggaggaatc cggcggactc atcaagctgc agctcggccc ggacggaagc ttactaaacc
    19021 tggagatacc gcccagcacc gaggaagtca ccgaggaggt cgaagagacc acacagcaga
    19081 ctcagcagac ccagcagggc ggcggaggcc agcagcaagg tggagaggaa ccggacgcca
    19141 ccgaggccgc gaagcagaag gccgaggagc tgggtgtaga cctctcccag atagaaggtt
    19201 cgggcgccgg cgggcgcatc accgtcaagg acgtggcggg cgccgcgggt taaaagagcc
    19261 cggtagcatt accgctgatc acgacggcgc cgggtatggg tgggcgagac cttgacttcg
    19321 tccgccccta tccggcgtct tgtagagttt gacgcaaggc tgtacaacgg cgcggatccg
    19381 tccaggaaag gagtcttatg gcagagagta ggggaaacgg gaaaggtgga gtgcaggaga
    19441 cgaaggcgtg gctcgtcaag tcggcgcgga tgctcgccat gtccgagaaa gccggggtct
    19501 ccgaacccat ggacgtcaca gggctggcgt tggccgcttt tgaggacatg ccgctgaatc
    19561 aggctctggt cttcatacgg accaacgagc agaacataag ggatctggcc tgggccttca
    19621 agaactccag ctccgccgaa gagttcgagg aacgggtgag agagatcaag cacctgtccg
    19681 actgagagaa ccggttgctg gcgagaccgt tatatggacg ccggatctcc tttatgtgga
    19741 accctcgttc tatcatcccc aaagtgttgt ggttctttta taacgaggtt tacaacgctt
    19801 cgcagcccgg catagaatgg accgaaaacc gggaggtcct ttttgagtgg aacggacggt
    19861 gtcgggcgga acaatcatat cgaagataga cgcatgtggc ggttgctcct ggcgcagttg
    19921 catcccgacg ccggcggaga tcacgagttg ttcctgtttg ccgccgctct gagagaaagg
    19981 tcgtgcggcg agtctacgga gagagactct gcccgggacg gcagcgagag agacgaacgt
    20041 gtctccgagc ctttcttaca ggcatggcac gatactatgg ccgcctgggc atcgcgaaac
    20101 cacgagagct tgagaagcct cgtacttggc gaaacccctg ttgccgcgag ggattagcca
    20161 ccgcaacagc agcgtagaaa cttggacctt aaaacagtgc aggtgctctc ttgcttctgg
    20221 cggacgggag gttcggctaa tatgctgaag ggtaaaaggg agccagtatg agtgtgcggg
    20281 atcaggaacg gcgtcaacgg gaagccgagc gcaggataag ccaggccatg aagagggccg
    20341 acagggaggt ccgcgacgcg atggaggcgc gggcggtagc cgcagaccgc aggaaggaac
    20401 aggtcaagag gaggatcgag ataggcgacg tcgatcctcg tcctctgccc ggccttcggg
    20461 aagattcgat ggagtcgatg ccttcgatgg agtccagcga gtcgataaag tcgatgcctt
    20521 cgatggagtc cgccgattac gcgagcgcgt tgaccgcaga agaagccaac gccgggggga
    20581 acatgatgca ggacatgccc ctggagggaa agatgcccat agaccccgcc cgcgcggagg
    20641 aagaactggc gcaagagctg accgaggaga cgggctccgg gggagatgtg catttgcacg
    20701 ccgagcgcaa ggcagacgag atggcaaacc agatgaccga ggacacgggc gctggaggag
    20761 atatggaact gcatgccgag cgtaaagtgg agcagatggg aaacgagatg ttcgaggact
    20821 aagagcgcct gactcgccaa cgacgcagcg gctcgttaac atcattatgc cgagtggacc
    20881 cgcgctcttg tagctcctgt aatctgttgt gatctgccga ctcggactaa tctgcgggcg
    20941 tccacaaaag cgacgcccac aacgacgatg cggtcgggag gttatctcgc ggaggttgcg
    21001 gaccccagaa cgttcaaact gttttgacct tcaggacccc tgctgctgct tttgcatttg
    21061 ttgaatcacg ttccatacaa gatcccctgc ttcccgctgt acctggatcg catcaccctg
    21121 ctgcgggtag ttgagctcca tcaagttctg cactttcctg atagcggtca gggcgctggc
    21181 cagttgctgt acagggctct gttgttgctg ctgttgacct tgttgttgct ggccttgagc
    21241 ttgctgtccc tgactctgct gttgaccttg ctggccctgc tgttgctggt tcccctgcgt
    21301 catcgcgacc tcctgaatcg tgctttgtat ggtaagtacc cggcagtcgc gtaaagtaga
    21361 ctcgcatgat gcctcgctca cgggtttaca tcatgttggc ggggggcagt agaggcggag
    21421 aacgcgacgt ttcatggagg ccgggaaccg tatgtctagg agcagaagac ctcgggatga
    21481 ggaacatccg gcacaggata gtgaggaacg gccggtagga gagcagccca gacagataga
    21541 agaagcggat gtgctgctcg acgtgccgga gctcaacgtg gaggaattca gcctccaggc
    21601 cgagcaggta cgggtgcgag tctccttcgg ggccgagctt gcggacatgg tcaagatcaa
    21661 cgtcggtctc gaggcggagg tggacgactt cgagctgaag gcaaagggga tcgaagccca
    21721 ggctcaactc aaggcccggc tcgggaacgt gcgtgagatc ttctcgcagg tcctctcaac
    21781 cctggacaac aacccgagtc tcattcagga tctgctcggc gccccggacc gcaagggaga
    21841 cgaaccggaa ggcgagccgg ccgagccgcc gcgtagagcc ctggaagaga gcccgcccga
    21901 cgattcagac gcttcggatg gcggcgacac cagggtcaat gccaggaggg atggtggcgc
    21961 gagactgact gaagacagcg ctaccaacgg accgcgaaaa tcggatgcga cggaggcggc
    22021 cgagaaaaag gcccggcagc ttgggataga actcggcagt ctggagggta cgggggtcgg
    22081 cgggcgtgtt ctgttaaagg atgtggtgga ggccgccagg aagtaagtag cttctaccac
    22141 acccgctctc cagcgttaca atacgttgag agcgtgagga ggatcaacgg atcatggcaa
    22201 aagactttaa acagagctcg gcagagatcc tctcggaact gctctcggat gagcgtgtga
    22261 agcgcgtgcg ggagtacgtc cagggagagg acttcaaggc cgaccgtgaa cgtgtgagga
    22321 agaatgccgc cgaccgattg aagaacatcc gggagcgcta cggcgcgaaa cgccgcgacc
    22381 ccgagacagc cgctcgtgag aaagaactta ccctgcgcct cgccgaagtc gaggccgagg
    22441 ccgccgagat ccgcatccgc ctctccgagc tactggagga agaaggtaag ctacggagcg
    22501 cgctggacga actgtagcgt tggcgctctt ctaatacgtt tttccagagg ctttatccga
    22561 tactagtatg ctacaggctt gctatccact tgtgctttgg tggggattcg tgtaaaaagt
    22621 gggttacttt cgtaccctgg gctctatagg aggcgatttg ttgaccccga agaccggact
    22681 ttacgacccc ttatacgaac acgatgcttg cgggttcggg tttgtagcgc gtatcgacgg
    22741 acgtcgcacg cgcgagacgg tcgaggaggg tctggaggtt ctgcacaacc tggagcaccg
    22801 gggaacgact ggagcggacc cggagacggg tgacggggcg ggtatcctga ctcagatccc
    22861 ggacggcttc ttccgccgcg agtgcaaaga actgggcatc gagcttccgc ccgcgggagg
    22921 ctacgcagtc ggagtggtct tcgagtttgg tgaggccgag gggcttgact gtgagggacg
    22981 gctcgcggag atctgcgcgg aggaggggca gaagctcctg ggattccggg aggtgccggt
    23041 ggtgccggag gcggtcggga agatcgcgcg gagcgtgatg ccgcgtgtcc ggcagttctt
    23101 tatcgagggg catgcgggtg acgaggatgc cttcgagcgg aagttgtacg tcatacgccg
    23161 tcggctacac aaggcggtag aaggcggtca cggctgctac gtggtgagcc tatcttcgag
    23221 gacggttata tacaaggggt tgctcaaggg cgagcagttg ccgcgattct atccggacct
    23281 cggggacccg gcgtttgcga gcgcgatcgc gctggtccac gagcggttct cgacgaacac
    23341 gctggggagc tgggagctgg cgcatccgta ccggtatgtc gcgcacaacg gcgagttcaa
    23401 cacgctgcgc gggaacgtca actggatgcg cgcccgcgag agccgcctgc aatcggacct
    23461 cttcggggag gacctgaaga agatatcgcc ggtcatccag accgggcaga gcgactcggc
    23521 gtccttcgac aacggtctgg aactcttgca cctggccgga cggagcctgc cgcacgcggt
    23581 ctccatgatg atgccggagg cctgggagaa tgatgagttg atggaccccg agcgccgcgc
    23641 ctactaccag taccacagcg cgctcatgga gccgtgggac ggcccggcgg ccgtggcgtt
    23701 cactgacggg cggctgatcg gggccacgct cgataggaac ggcctgcgcc cggcgcggta
    23761 ctccgtcacg aaggacggac gggtcgtaat ggcttccgag gacggagcgc tcaggattcc
    23821 ggccgaggag gtgatcgagc gctggcgttt gcagccgggg aagatgctcg tcgtggatac
    23881 ggagcggcac gagctcttgc acgacgagga ggtgaagaaa cctctcttcg cgcggcatcc
    23941 ctaccgggag tggctggagg acggagagat ccacctgaat gagcttccag aggctgattc
    24001 ggaacggcgc ccggagtccg cgtcgctgtt cgagcggcag caggcgttcg ggtacactat
    24061 cgaggatcag cgcataatcc tcgctccgat gggcatggac ggcaaggagc ccgacggctc
    24121 gatgggcacc gacacgccgc tcgcggtcct ctccgagagg ccgcaaatgc ttttctcgta
    24181 cttcaagcag cacttcgcgc aggtcaccaa cccgccgata gatccgttgc gcgaggagct
    24241 ggtcatgtcg ctcaagatga gcctcgggcc ggaacagaac ctgttcgacg agacgcccga
    24301 gcactgccgc agaatcctca tagaccagcc catactaacg gcggctgagc ttgagaagat
    24361 ccgtcacctc ggcaccgagc gcgcttccac caccaccctc tcgaccctct tctccgtctc
    24421 gtccggcgag aaggggatgg aggacgcttt gacgagcctg tgcgagaggg cggttagcgc
    24481 ggtgagggga gggtctgcgg tcctggtgtt gagcgaccgg ggcgttaacc cgatgcaggc
    24541 ttccataccg tgcctgttgg ctacgtcggc cgtgcaccat cacctggtgc gagcgggcat
    24601 tcggacggcg acgacactcg tcgtggagac cggggaggcg cgtgaggtac accacttcgc
    24661 tttgctcgta ggctacggcg cgaccgcaat agcgccttac ctcgccttcg agacgataga
    24721 ggatctggtc ctgtcggacc accttatgga gggcgtgagt ccggaggagg ctgcgaagaa
    24781 ctacgtcaag gccatcggta agggcctgct gaaggtcatc tccaagatgg gtatctcgac
    24841 gctgttctct tattgcgggg cacagatctt cgaggccgtc ggcctggaca aggacttcgt
    24901 ggagaagcac ttcacgggca cggcttcgcg cgtcgggggg gtcgggatcg gggctctcgc
    24961 gcgcgaggtg ttggagcggc accgcaacgc cttcggcggc acggaggaca acgcggagga
    25021 gctggagatc gggggcgagt accagctccg ggcgcagggc cagtatcacc agtggaacca
    25081 gaagagcatt ccggccctgc agcgcgccgt gaagacgaac gatttccaga cgttcaagga
    25141 gttcacccgg cacttcgacg agcagagcgc gcgcttctcc acgctgcgcg gtctcttcga
    25201 cttcgaggaa gccccgatcc cgctggagga ggtcgagccg gcgaaggaga tcgtcaggcg
    25261 cttcgttacg ggcgcgatgt cgttcggttc catctcgaag gaggcgcacg agacgctcgc
    25321 catcgcgatg aaccgcatag gcggcaagtc gaacaccgga gagggcggcg aggatccggc
    25381 gcgcttcacg cccgacgcga gcggcgacaa ccgtcgcagc gccgtcaagc aggtcgccag
    25441 cggccggttc ggcgtgacga cggagtacct cgtcaacgcg gacgtgttgc agataaagat
    25501 ggcccagggc tcgaagcccg gcgagggggg ccagcttccg ggccacaagg tctcggagga
    25561 catagcgaag gtgcgctact cgacgccggg cgtcggcctt atctcgccgc cgccgcacca
    25621 cgacatctac tctatcgagg acctggcgca gctcatccac gacctcaaga acgccaaccc
    25681 ccgcgccgac gtgagcgtga agctcgtggc ggaggtgggg gtcgggacgg tcgcggcggg
    25741 cgtggcgaag gcgaaggcgg accacatcac gatctccggc cacgacggcg ggacgggcgc
    25801 ctcgccgctc tcgtccatca agcacgcggg gcttccgtgg gagctcgggc tgtccgagac
    25861 gcagcaggtg ctcgtccaga acaacctgcg cgggcgtgtg atcctgcaga ccgacggcca
    25921 gctcaagacg ggccgcgatg tggtggtcgc ggcgctcctg ggcggggagg agtttgcgtt
    25981 ctcgacggct ccgctggtgg cgacgggctg catcatgatg cgggtctgcc acctgaacac
    26041 gtgcccggtg ggcatagcga cccaggaccc ggaactcagg aagcgtttca cggggacgcc
    26101 ggaacacgtc gtcaactact tcttcttcct ggccgaggag gtccgggagc tgatggcccg
    26161 gatgggcttc cgcacgtttg acgagatggt cggtcgttcg gacatgttga agatgcgcga
    26221 ggccatcgag cactggaagg cgaagggtgt ggacctgtcg gccattctgc accaggaaga
    26281 ggcgttggag ggcggggaga tccggcactc ggagacgcag gatcacgggc tggagaagtc
    26341 gctggacaac gagttgatcg aacgctgcat gcctgcgctg gaggagaagg agccggtccg
    26401 cttctcgcag gaggtcgcga acacccaccg cacggtcgga gggatgctct ccggggagat
    26461 agcgcgccgt tacggcaacg acggcctgcc cgaagatacc atccgtatag atatgcgcgg
    26521 cgttggcgga cagtccttcg gggcgtggct ggcgaagggc ctggcgttta gtctcgaagg
    26581 cacgaccaac gactatgtcg ggaagggact ctccggcggc aagctcgcgg tgttcccttc
    26641 cagagtcgcc gcctacgatc catcccgaag cgtcgttgtc ggcaacgtgg cgctctacgg
    26701 cgctacgggc ggtgaggcat atttccgggg cttcgccggc gagcgcttcg ccgtccgcaa
    26761 ctctggcgcg cacgcggtcg tcgagggtgt cggggatcac ggctgcgagt acatgaccgg
    26821 cggggtcgtg gtggtgctgg gtccgaccgg gcgtaacttc gccgccggta tgagcggcgg
    26881 cgtggccttc gtgctggacg aagaggaccg tttcgagaag ctttgcaacg tggatatggt
    26941 gagcctggag gcggtcgagt cagaggagga cgaggccctg ctgcggagca tggtcgagcg
    27001 gcatctggag tggaccgaga gcgacgcggc gcggcgggtc cttgaagact gggaggaact
    27061 actgccgaaa ttcataaaga tcatgccgaa cgacctcaag cgggtgttga gggagcggca
    27121 ggaggctgag ctggaggtgg cgcgctagtg ggcgacccga agggattcct caagatcggg
    27181 cgcaagccca ccccgaagcg caccatcgag gagcgggtgc aggactaccg ctacatctac
    27241 gagccgatgc cggaggacga actgaaggga caggcatcgc gctgcatgga ctgcggcgtg
    27301 ccgttctgca acacgggctg tcccctcggg aacctcattc ccgactggaa cgacctggtc
    27361 tataaggacc actggaagac ggccatagac cggctgcaca agaccaataa cttcccggag
    27421 ttcaccggga tgctctgccc cgcgccgtgc gaggccgcgt gcgtgctcgc catcaacgac
    27481 aagcccgtca cgatcaagga gatagagctc tcgatcatca accgcgcctt cgacgagggc
    27541 tgggtggtgg cccatccgcc gccgccggag aagcggaccg gcaagaaggt cgcggtcgtc
    27601 gggagcggtc cggcgggcct tgcggcggcg cagcagctaa actgggccgg acactcggtc
    27661 gtcgtcttcg agaaggacga taagatcggg ggcctgttga gatacggcat cccggactac
    27721 aagatcgaga agtgggtcgt agaccgccgc atcgccatca tggaggagga aggcgtcgag
    27781 ttccagacta acgctcacgt cggcgaggac ccgacgacgg aggagctgaa gagcgagttc
    27841 gacgcggtga tcatggccgt cggcgccttg caggggcgtg acctggacgt gccgggacgc
    27901 gagcttggcg gcatccacct ggcgatggac tacctcacgc agcagaaccg gcgggtggcc
    27961 gggcttccga cgaacgcgga ggtggagatc tccgctaagg gcaagaacgt ggtgattctg
    28021 ggcggcggcg acacgagcgc cgactgcctg ggcaactcgc accgcgaggg ctgcgcctcg
    28081 gtaaaggtgc tcacccacgg cccgaggcct cccgagaacc cggaccacct cacctggccc
    28141 gactggccgt tcatcctgca cacctacccg gtccacgagg agggcggcga gcgcggctgg
    28201 agcgtcgccg tcaagggctt ctccggctcg aacggccacg tcgagaggat gcacgccgtc
    28261 cggaccgagc gcgagaacgg taagaccata cctatcgagg gtacggagtt cgagatcgag
    28321 acggacctcg tgctgctcgc tatcggcttc accgggccag tgcgtgacag gcttcaggag
    28381 gagttggacc tcggttatac ggaccgcggc gcgatccact ccgagaacgg ttacgccacc
    28441 cgcgagcccg gcgtcttctt cgccggcgac gccaagcgtg gcgcttccct tatagtctgg
    28501 gccatcgccg aaggccggaa ggccgcccgt caggcggacg agcacctcat gggctacagc
    28561 ctgctgctcg ggtaggtttt tctccacaca agctgattcg ccagtcgctc tgccggtgag
    28621 aagccggcag tatgataatg cctcccgaga gcgagtctgc attcgctctt cgcgtggtta
    28681 tcctcctgca taacaaccct ctcaacgccg cgcgggattc ttatggaccg ggccgaagac
    28741 tcgtcagggg acacgtctgt aagttactcg cagcacatat gtgcatgttg taaaagagca
    28801 ggcaaccatt gaaaggagat ggcacggcat ggcccggata ccgatctggc aggacgatac
    28861 accggataca ccgccggaga tcaccgaggc gatagagtac gcgaagggcg gcacgggctt
    28921 cgccctcaac atccagcgcg agatggcgaa caatccgaag atcctcaagg ggttcagcga
    28981 tctcgtcgcg accttctatg gcgaagaggg cggcgccatc accgctgcgg agcgggagct
    29041 ggcctacacg acagcgaccg tcgtcaacaa ttgccactac tgaataccca cgcatatcat
    29101 gctcggtcgg agcgtgggac ttactactga acagctcaac cacctgggag acgacccgct
    29161 gccggaaggc gtctacagcg agtccgaggc cgcgatagtg cgctacgccc aggtctctac
    29221 cagcagcatc ttcatcaacg acgagattta cgatgagctt gcgagacact acacggacga
    29281 gcagatcgtg gagatctgca tagtcgtcgg tctcagcaac gtggtcaacc gcttccacgc
    29341 gacgttcctt acggacgtgg atgaggatac gacaaaggcg aacgccgaat cggatgcaca
    29401 cctgggcatt tgtactctac cgcgcccgag tgcggtacag gtctagaaga agtttcgccc
    29461 ttctgtacaa gcagcccgcc ggacattacc ggaaagtttg tccggcgggc taaagacccc
    29521 gagacgagta tttcctagag tacttcgagt atcgctaggg ttccgccgcc ggcaggcgcc
    29581 ggcgggtcag tgaccgagag cggtaagcgg ccgggagaca tccggccgcc acacggcggg
    29641 ataaaagccc gggaggcagc gagaagagct tcccgggtgt tcgcatttcg gggagcggaa
    29701 cagctaccgg aaggagcgag tcttgggagg aaatcaccac catcaccacc ggagggtcgg
    29761 gccgccggac ccggaggcgt tgaagcggct gcgcggctac gaagcaaggg agcgcgaggc
    29821 ggcgctgggg agcgcccggt gggacgcgga ggtggagcgg tcgctggaga tcggtcttcc
    29881 gggcgcggac tctatagaag ataagaccat ctcgacgttc tcgcggacgg agcttccaca
    29941 cttcgcgggg atcaacacgt tcatgagctt cccgtacctg gaggacgtgc gcgaggtcgg
    30001 caactacgac gtggcgatcc tcggggcgcc gttcgacatg gggacgactt accgctcggg
    30061 gacgaggttc gggccgcagg cgatacgaag gatctccgct ctctactcca cgtacaacta
    30121 cgagttcgcg gtggacctca gggagtcgct gaaggtggcg gacctggggg acgtgttcgt
    30181 tatcccggcg aacatcgaga agacgttcga ccagatcgca aacgccgtca gccacgtggt
    30241 cgagagtggg gtattcccgg tgatcctggg cggcgaccac tcgatcggct acccggacgt
    30301 gcgcggcatc gcgccgcaca tcgacggcaa catcggcatc atccacctgg accggcacgt
    30361 ggacatccag gagaaggaca tggacgagcg tatgcacact acgccgtggt tccatgcgac
    30421 gaacatcccg aacgccccgc cgaagaacct ggtgcagtgc ggaatcggcg gctggcaggt
    30481 cccgcgtagc ggcgtcacgg tcgcccgcga gcgccagacg accatcatga ccatcggaga
    30541 cgtggaggcg atggggattg agaaggccgc cgagatggcg ctggaggtcg cctgggacgg
    30601 cgcggaggcg gtctacctct cgttcgacat cgactccgtg gacggcgggc tggtgccggg
    30661 tacgggctgg ccggagccgg gcggatacct gccgcgggag gcgctcaagt tcctgcacct
    30721 ggtggcgaag gagggactgt gcggcatgga ggtggtggag gtctcgccac cctacgacac
    30781 gagcgacacc acggcgctgc tcgcggcgcg ggcggtagcg gacgtactgg cgacgctggt
    30841 caccgagggg cacctcggga cgtatccggc ggtgtagaga ggaggcgagc atgatcgttt
    30901 tcggagtagc gttctttatc tttttgcagg tggctatcgg attgcagttc gtccgcaggg
    30961 tgcggggcag cagcaccaac ttcatcgtcg cgggacgtgg attaatcttg ccgttcgccg
    31021 ccgccgctct gatggcgcag gcggtcgact ctaacgcaac tttaggcaac acggacctct
    31081 ccgcgcagtt cgggttctgg gccggggctt cgctcccgat agggctcgcg ctgtgtctct
    31141 tcctgacggg gctgttcttc gccaagccga tgaaccggat gggcctcctg acgctcccgg
    31201 atttctaccg ccgcaagtac gggcgtacgg tggagatgct ggcggctccg atcatggtgc
    31261 tcagcttctg catcctgctg gcaggcaacc tggtcgcggg cggctaccta tttcaggctt
    31321 tcctgggaac gagcttcctt gtaggggtct tgatcatcgc ggcggtcatg ctcctgtata
    31381 cgctgccggg tggtctgctc tccgtggtct acacggacct cttgcaggcc acgctcgcgt
    31441 tcctgggggc cgtggccctg ctcgtgttcg tcgccatgaa ttacgggatc tccatccccg
    31501 acggcatggg accgttcgac ttcgagcagc taacagaccc gaaggtgggc gcgtacataa
    31561 actgggcgac gctcatcgcg ctcggcctgg gagacatcgt cgcgctggac ttcatggagc
    31621 gcgtcttcgc cgccgagagc ccgcggacgg cccgtcgggc gtgctacgtc ggcgccctgg
    31681 gcacgctgat tatcgggata ccgttctcca tcgtggcgct ttcggcgggg agcatcttca
    31741 aggagctggg ggtaaagccg gacggcccgg tgctcttcgc gctcttgcag gacacagcgc
    31801 cggccgtgat cgtggtgatc gtactctccg gcgtggtggc ggcctcgctc tcgacgggga
    31861 ccggcgcaat cctcgccatg tcctccgtct gcgcgcgtaa tatcctgggc atccggccgg
    31921 gagagggcgc cggacgcgac cggctactcc tgatcaccag gatcgggatg atacccatcc
    31981 tcctcttcgc cgtgatcatc gccctgcgcg tcccccagac gggcatcctg ctcacgctcg
    32041 ccttcgacgt gaccctcgcc ggcctgctgg ttccgttcgc gctcggcatc tactggtcca
    32101 agtccaacgc gcccgcggcc ctagtcgcca tcgtcgccgg ttccgtgacg cgcctggtgc
    32161 tcttcgtcct gacccccacc acctacggcg tcgagaacac cctgctctac atcccgaaca
    32221 agatcttcag cgcctccttc gatgggatac ccacaatcct gagcgccctc gtcggcctcg
    32281 cggccttcgt cggcgtggca ctcgccaccc agaagagcca cgagcccgtt accctcgacc
    32341 tccacagccg ggaaccagag cctgcggtcg cggaaggcta gagaacctcg tccctcccgg
    32401 agtacaaaac tcagacactc cgggagggac ctttctggta aacgggccaa agacctcgtt
    32461 cccaacctcc ctcacaatat gaaaagtcat atagattcgt agtgcatcta tagggaggtc
    32521 tgctggatga tcggcgagca ggaacgtcag ggaagcggcg taaagcggaa gttcaactcg
    32581 ttcgtggaca acccggtgct ggaggattac tcgctgcggt acgcgccgcg ctctttcagg
    32641 aagtggagcg agtatgcctg cgcgacggcg gctctcgggg ggatagcgta tctggtggac
    32701 ttcgccatcg gggcttcgat cacggtctcc tttggattca ccagcgcgac gctcgccatc
    32761 ctgctcgccg ccgtcaccat cttccttacc ggaataccga tagcttatta ctcggcccgc
    32821 tacaacattg acatggacct gcttacccgt ggggcgggct tcggatatct ggggtccacg
    32881 atcacctcgc tcatctacgc gagcttcacg ttcatcttct tcgcactcga agcgtcggta
    32941 atgtcccagg cgctgaacct gctattcggg cttccgttgg cgctggcgta tgtgatctgt
    33001 gctttagcga tcatcccgtt cgtggtcttc gggatgacgc tgctctcgaa gttgcaggtt
    33061 tggacgcagc cgatctggtt cgctctcata ctcgcgccgt tcgtcgcgat ccttttcatc
    33121 gagccgggtg tctactccga ctggacgagg ttcgccggga cggagaatca tacggctggc
    33181 ttcgcgctgc tgggctttgg cctcgcggcg ggcgtggcgc tctcgctcat cgcccagatc
    33241 ggggaacagg tggactacct gcggttcatg ccggagaaga ccccggagaa cagcttcagg
    33301 tggtggacgg cggtcctggc ggccggtccg ggctgggtca tactgggcgc gctcaagcag
    33361 atgggcggtg cgatcctggc ggtcttcatt atctcggagg ccggacctga tggggcggtc
    33421 gagccgatcc ggcagttcgt cagggggttc gagctgatca tcccgaacgg cacggcggct
    33481 ctggcggtgg cgaccgtgct ggtggtgctc tcgcaggtca agatcaacgt gaccaatgcc
    33541 tactcgggtt ctctatcgtg gtcgaacttc ttctcgcggg tgttgcactg gcatccgggg
    33601 cgagtgtggt gggtcttctt caacgtcggc atctcgctcg ccttgatgct cggcggggtc
    33661 ttcggtttcc tgaacttcgt gctcggcttc tactccaacg ttgctatcgc gtggatcggg
    33721 gccgtcgtag cggatctcgt gatcaacaag ccgttcttga aggtgagccc ctcctacatc
    33781 gagttcaagc gggcgcacct ctatcaggta aacccggtgg gcttcggcgc catgatcgcc
    33841 ggcgcggcgg tatccatacc ggcgttcttc ggcgtattcg gcgagtacgc tcaggccttc
    33901 tcgccgtacc ttgctctgac catcgcgttc gtcctctctc cggtgctcgc gctcgctacc
    33961 cgcggcaagt actacatcgc ccgcgagggc tcgaccagcg cgcgcctgga gagcggggac
    34021 gaccgttacg cgctgaccac ttgcagccag tgcgggcagg gctatgagac acccgacatg
    34081 gcgcactgca cgttccacga ggagcagatc tgctccctgt gctgcaccct ggagaagacc
    34141 tgccacgacc actgcaagaa gcctcttgag atagggagaa agcccggcgc ggagccggcc
    34201 tgatccggac ctcacgcagc ctaccctggc gggaagctac agactgtccc accagggttt
    34261 gtaccaatgt ccaaagaacg gcgcttcctg gactgcccca tttggacttt cggccggaga
    34321 ctatatcgtg agtagaacct aagattctcg catccattgt ccgatgttcg cgttcaggac
    34381 ggagccaggg tagaacccgg aggaggggaa tgaagctctc accgatggag caggagaagc
    34441 tcatgatctt caccgccgcc gaggtggctc gtcgccgccg cgagcgcgga ctgaagctga
    34501 actacccgga ggccgtggcc atcatctccg ccgagcttct ggagggcgcc cgtgatggtc
    34561 agagcgtcgc ccagttgatg agcttcggcg cgaccatact ctcccgcgac gacgtgatgg
    34621 aaggagtccc ggagatggtg gacgaggtgc agatcgaagc caccttccca gacggcacca
    34681 agcttgtcac catccacgac cctatagtct agggaggacg cgatgaaccg cgaagaggcg
    34741 acccaactca tactcgaagc gaagcgcgag aagagcctga ccttcgaagc catcgcgaag
    34801 aaggtgggcc gtcataaagt gtggaccacg gcggcgctga tgggccagca tccgatgagc
    34861 gccggggaag ccgacgccgt ggtaagcctc ctggggctgg aacccgaagt agtcgcggtg
    34921 ctgcaggaag tcccgatgcg cgggagcctc agctcaaccg tgcccaccga ccccacgatc
    34981 taccggatac acgagatcac gcaggtctac ggcaccacaa tcaaggccct catccacgag
    35041 gagttcggcg acggcatcat gagcgccata aacttcaaca tggatataga gcgggtcgag
    35101 aacccggagg gcgaccgcgt caggatcacc tacgagggca agttcctgcc ctatcagtgg
    35161 tgaagccatg atccccggcg aacttctccc aaacgaaggc tccgtggaac tgaacgcggg
    35221 ccgcgaaaca aacgccatca ccgtcgccaa cacgggagac cggcccatcc aggtcgggag
    35281 ccacttccac ttcttcgaga cgaatcgcgc gctcaagttc gaccgtgaga gcgcctacgg
    35341 gatgcgcctg aatataccgg cgggcacgtc ggtgcgcttc gagccgggcg acgagaggga
    35401 ggtagagtta gtcgccatag gaggccgcag ggtcgtgcgc ggcctgaacg ggctcgtcga
    35461 gggcgcgctg gacgatgcgg acgttagaga tgcggcgttc cgccgggcgc gagagcaggg
    35521 attcatggag gaagcatgag cgatctttcc agaaggcgct acggtgggat gttcgggccg
    35581 acagttgggg accggctgcg gcttgctgat acgtcgctcg ttgtcgaggt cgaacaggac
    35641 ctgacgctgg ccggggagga ggcgaccttc gggggcggca agtccgtgcg cgaggggatg
    35701 gcgcagggcg ccgccacacg cgaagagggc gtgatggatc tcgtcatcac gaacgccctc
    35761 atcctggacc actgggggat cgtcaaggcc gacgtgggcg tgcgggacgg gcgcatcgcg
    35821 gctatcggca aggccggaaa tccggacacg atggacggcg ttacggtgga gatcggcgcg
    35881 tccacggaga tcatcgccgg ggaaggcaag ctgctgaccg ccggagggat agacacccac
    35941 atccacttca tctccccgca gcagacgacg cacgccatag cgtccggcgt cacgaccatg
    36001 atcgggggcg gcaccggacc ggcgaccggc acgaacgcga cgacttgcac gccgggagcc
    36061 tggaacatcg cccggatgct tcaggccgtc gaggacctgc ccctcaactt cggcttcctc
    36121 ggaaagggga actcctcgaa cccggaggct ttgcgcgaac aggtccttgc cggagccgcg
    36181 ggcctgaagc tgcacgagga ctgggggacg acgccgcagg cgatccggac gtgcctcacg
    36241 gtggcggacg atctggacgt gcaggtcgcc attcacaccg acaccttgaa cgagtccggc
    36301 ttcgtggagg acacgacggc ggcgttcgag ggacgcacca tccacaccta ccacaccgag
    36361 ggcgcgggcg gcggccatgc gccggacatc atcaagctgt gcggcgaagc gaacgtgctg
    36421 ccgtccagca ccaacccgac ccgcccctac accgtcaaca ccgtggacga gcatctcgat
    36481 atgctcatgg tctgccatca ccttagccgc ggtgtcccgg aggacgtatc cttcgccgag
    36541 agccgcatcc gggcggagac catagctgcg gaggacgtgc tgcacgatct gggcgctctc
    36601 agcatgatgt ccagcgactc gcaggcgatg gggcgtgtgg gggaggtctg gatccggacc
    36661 ctccagaccg cgagcaagat gaagacacag cgcggcccgc ttcccgaaga caccgaccgc
    36721 aacgacaacc accgcgtgaa gcgctacctc gccaagtgca ccatcaaccc ggccataacg
    36781 cacggcgtcg cggaccatgt cggatctatc gagaccggca agcttgcgga cctgatcctc
    36841 tggagcccgg ccttcttcgg ggtgaagccg gagatcgtgc tgaagggcgg cttcatagcc
    36901 tacgcccaga tgggcgaccc gaacgcctcc atcccgacgc ccggcccggt cttccagcgc
    36961 ccgatgttcg gcgcgttcgg ctccgctccc gccgcgactt ccctgacctt cgtttcgggg
    37021 tacgccgccg agaacgggct gagagagagc ctgggattga agaagcaact tactccggtg
    37081 cgtaatacgc gcgctctcgg caagacggac ctgatccaca acgacctcct cccggacatc
    37141 gaggtagacc ccgagaccta cgaggtccgc gtggacggcg aggtaatcac ctgtgagccc
    37201 gccgccgagc ttccgttggc ccagcgctac ttcctcttct gatgcgaggc gaagccttgc
    37261 tgcgcgtgct ccaggtctcc gacaccgcct tcccgaccgg ctccttctca cactcgatgg
    37321 ccgtggaggc gttcgccgaa gccggagaat tacaacacgc tgaagacctg aagcgcgtcg
    37381 ccggactcca tctctcgaca atggcgacct cggactgcgt cgcgctccgg gcggcgttcg
    37441 gagcggagtt ggaggagttg tttcggatag accggctcct cacggcaacc aggctcaccc
    37501 gtgagcttcg cgcggccagc tcttcgacgg gcaggcgttt cctgttgagc gtcgccgctc
    37561 tgggtgtcga gggtgcggtg ctcggggcct tcacggacgc cgcgcggaat agtgagacac
    37621 cgggcaacct ggcggtcgga tatggggtcg cggctccggc gctgggactt actctggacg
    37681 agacgttgcg cgcatatctc tactcgacgg cggcttctct cgtcgcggcg gggcagaagc
    37741 tcatccctct aggcgggagc gcggcgcagc gggtgctgta cgagttggga gataagatcg
    37801 agatcgcagc gaagaccagt gagacgatgg aggtagaaga aatgtatgcg tttgcgccaa
    37861 ctatagacgt gcgctcgatg atgcacgagc gccagcggac aaggctgtac atctcgtgag
    37921 cgcgcgggag ctagcgctga ggatcggtat cggcggtccg gtaggctccg ggaagacggc
    37981 gctggtggcg gagctttgcc gcaggctcgg ggacgagctc gcgatagccg ccgtcaccaa
    38041 cgacatctac actcgcgagg acgccgagtt cttgcagcga gccgccgtgc tcccggaagg
    38101 ccgtgtcgtc ggcgtagaga cgggaggatg tccgcacacc gccatccggg acgacgtgtc
    38161 gatgaacctg gaggctgtcg aagcgttgga gcggaagttc agtctcgacc tgatcttcgt
    38221 cgagagcggc ggagataacc tggcggcttc cttctccccg gaactggtgg atgcctcgat
    38281 ctacgtgata gacgttgccg gaggcgacaa ggtccctcgc aagggcggtc ccggcgtaac
    38341 ccgctcggag ttgctgatca tcaacaagat agatctcgcc cctcttgtcg gagctgacct
    38401 ctccgtcatg gaccgggacg cgggcgccct gcgcggcgag gctccaacgc tcttcgcctc
    38461 cgtgttgcac ggcgacggta tggacgccgt agtcgggtgg gtgcgcgagg cggtgctcga
    38521 tcgccgatgg gtcccggcta cctcaactgc caggagagac catcatcacg agcatcccca
    38581 ctcgcactag agagacggga caacgcggct ccctccgtct ctcgttcgag cctcggggta
    38641 acagaacggt cctcaaggag cggtacgcat cggcgccgtt cggggcggtt cgagcaaatt
    38701 atccggacgg ctctggcatt cccgaagtcc agatcaccaa cccctccggc ggcattctcg
    38761 gcggcgactg cctggaactg gatgtctctc tcgcccccgg ctcctcggcg acggtcctca
    38821 cccaggccgc caacaaggcg taccggggaa cgcgcgcgag ccagcaaacg gtactccgtg
    38881 ccggagaggg cgctcttctg gagtatctgc cgcaccacct gatcccctac tctggttcat
    38941 cctacagcca gcggaccatc ttccatctcg cccccggcgc cgcgctcatc acctgggacg
    39001 cgtactccgc cgggcgcatc gcccgtggcg agcgcttcgc ctttgactcc ctccacggca
    39061 ggaccgccat cttccgggac gccgtcccgg aagctataga cggcttcgag cttatcgcgg
    39121 gaggagagcc gtttggcggc tatgcctaca tggcggcggc gtacatctcg aagccggagc
    39181 ctctgggagc actggccgac gagttgcacg catcgttggg cggtatgccc ggcaccctcg
    39241 cctcggccag cgctccatca cctcacctct gcgtcgtgcg agtcttgacg catagcgccc
    39301 ggtacctgta caggctcctg aacctcactc gccacacggc aagagaattc ctgaaactgc
    39361 ccgctcccgc ccgcg
//
LOCUS       DASSMB010000190         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1689455, whole genome shotgun sequence.
ACCESSION   DASSMB010000190 DASSMB010000000
VERSION     DASSMB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1689455"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VFJ72_11410"
     CDS             <1..245
                     /locus_tag="VFJ72_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7480111.1"
                     /translation="IANNTEYGLVAAVWTRDVGKAHWLAREIRSGQVFVNTYGAGGGV
                     EMPFGGFKKSGHGREKGYEALYEYSQVKTVAIKYGT"
     gene            complement(302..2083)
                     /gene="typA"
                     /locus_tag="VFJ72_11415"
     CDS             complement(302..2083)
                     /gene="typA"
                     /locus_tag="VFJ72_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET7480112.1"
                     /translation="MEYRNIAIIAHVDHGKTTLVDGMLRQTLQLGHGQEITERALDSN
                     VLEKERGITILSKNTAVEYGGVKINIVDTPGHADFGGEVERVLGMVDGCLLLVDAAEG
                     PMPQTRFVLRKAIELGLKPIVVVNKVDRQDARPEEVVDLTFDLMAELGATDEQLDFPI
                     LYAVAREGRAFMDIEEPREDMRELFETILEQIPAPEVGLAEPFQMLVANLDYSDYLGR
                     IVVGRVRRGTVRRGEFVNLIHKDGTMTKTRVVQPFTHLGLARIEAEEVGAGDIVALSG
                     IEDAQIGETVADLADPEALPVITVDEPTVSMIFQPNTSPFAGTEGRYVTSRHIQDRLK
                     REVQTNVSLRVEELRPDEFKVSGRGELHLSILLETMRREGYEVQVGSPQVITREINGK
                     KHEPIEHLVIDVPEQFASALIGTLSSRKGQLVEMEPQGARTRIEFKIPSRALFGFRTE
                     FLSMTQGEGIMSHVYDGYAPWAGDFKTRRNGSLVASEAGSAFAYSIWKLQERGRFFIT
                     PATEVYVGMVVGANSRENDLNVNVCKNKKLTNVRSAGADDALNLTPVRPMTLEDALEY
                     IGEDELVEITPESIRLRKKVLEPGMRK"
     gene            2314..>2675
                     /locus_tag="VFJ72_11420"
     CDS             2314..>2675
                     /locus_tag="VFJ72_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480113.1"
                     /translation="MDNKDEQIGDGMKETRWEGSPPGPGRLLAAKPFRPHPLLRGGHA
                     QTVGGWAWPRRFRPHPVDEPRLFEVEPGVRLLARCRWHEDRLARTTLVLVHGLGGSAD
                     APYMLGTALLAYKAGANVV"
BASE COUNT          435 a          871 c          860 g          509 t
ORIGIN      
        1 agatagccaa caacacggag tacggcctcg tggccgcggt ctggacgcgg gacgtcggga
       61 aagcgcactg gctggcccgg gagatccggt ccggccaggt gttcgtcaat acctacggcg
      121 cgggcggcgg tgtcgagatg ccgttcggcg gcttcaagaa gagcgggcac ggccgggaga
      181 agggctacga agccctttac gagtacagcc aggtaaagac cgtagccatc aagtacggaa
      241 cctgagactg cctcgacggg ggctttgcct ctttgaagcc cccgtcgtca gaacctgaag
      301 cttacttccg catccccggt tcgaggacct tcttgcgcag tcggatggat tccggcgtga
      361 tctcgaccag ctcgtcctcg ccgatgtatt cgagggcgtc ttcaagggtc atcgggcgga
      421 ccggggtgag gtttagcgcg tcgtcggcgc cggcggagcg gacgttggtg agcttcttgt
      481 tcttgcacac gttgacgttc aggtcgttct cgcggctgtt cgcgccgact accatgccga
      541 cgtagacctc ggtagcgggc gtgatgaaga aacggccgcg ctcctgcagc ttccagatcg
      601 agtacgcgaa cgcgctcccg gcctccgagg cgacgaggct gccgttccgg cgcgtcttga
      661 agtcgcccgc ccagggcgcg tagccgtcgt agacgtggct catgatgccc tcgccctgcg
      721 tcatggagag gaactccgtg cggaacccga acagggcgcg ggacggtatc ttgaactcta
      781 tccgcgtgcg tgcgccctgc ggctccatct caacgagctg gcccttgcgg ctggagagag
      841 tcccgatgag cgccgaggcg aactgctccg gcacgtcgat taccaggtgc tcgatgggct
      901 cgtgtttctt gccgttgatc tcccgcgtga tgacttgcgg cgagccgacc tgcacctcgt
      961 acccctctcg gcgcatggtc tcaagcagga tggagaggtg caactcaccg cgtccggaga
     1021 ccttgaactc atcgggacgt agctcctcca cgcgcaggga gacgttagtc tgcacctcgc
     1081 gcttcagacg gtcctggata tggcgagagg tgacgtatct gccctccgtg cccgcgaagg
     1141 ggctggtgtt cggctgaaag atcatgctga ccgtcggctc gtccacggtg atgaccggaa
     1201 gcgcctcggg gtccgcgagg tcggcgaccg tctccccgat ctgggcgtcc tcgatgccgg
     1261 agagggccac gatgtctccc gcgccgacct cctcggcctc tatgcgcgcc agccccaggt
     1321 gtgtgaaggg ctgcaccacg cgcgtcttcg tcatcgtgcc gtccttgtgg atcaggttca
     1381 cgaactcgcc gcgcctcacc gtgccgcgtc gtacccggcc caccacgatc cgtcccaggt
     1441 aatccgagta gtcaaggttt gcgacgagca tctggaaggg ctccgcgagg cccacctcgg
     1501 gagccgggat ctgctccagg atggtctcga atagctcccg catgtcctcc cgcggctcct
     1561 ctatatccat gaaggcccgg ccctcgcgcg cgaccgcgta caggatcggg aagtctaact
     1621 gctcgtccgt cgctcccagc tcggccatca ggtcgaaggt gaggtcgacg acctcctcgg
     1681 gccgggcgtc ctggcggtct accttgttga ctaccacaat cggtttgagg cccagctcga
     1741 tagccttcct gagcacgaag cgcgtctgcg gcatcgggcc ctcggccgcg tcgaccagca
     1801 acaggcagcc gtccaccatg ccgaggactc gctcgacctc gccgccgaaa tcggcgtggc
     1861 cgggcgtatc cactatgttg atcttgacgc cgccgtactc cacggccgtg ttcttggaga
     1921 ggatggtgat gccgcgctcc ttctccagga cgttgctgtc gagggctcgc tccgtaatct
     1981 cctgcccgtg gccgagttgc agcgtctgcc ggagcatgcc gtccacgagc gtggtcttcc
     2041 cgtggtccac gtgcgcgatg attgcgatgt tacggtattc catgcgttct cctgacccaa
     2101 tcgtcttacc gcgacgtaaa cgccttgcag gacacacgtg ccggagatca aggcaaaaga
     2161 aaaggccccg cggtgcgagc ctctgaccca gactataccg catctcgctc caggtacgac
     2221 cgcataattt cttcctcgtc ctaacgttac ttttgagatg cgcgttcatc gaatatatgc
     2281 ttctggcaac acttcggccg gaggcaagga ctcttggaca acaaagacga gcagatcggc
     2341 gacgggatga aggagacgcg ctgggaagga tctccgccgg ggcctggccg tctgctcgcg
     2401 gcgaagccgt tccggccgca cccgctgctg cgcggaggcc atgcccagac ggttgggggc
     2461 tgggcgtggc cccggcgttt ccggccgcac cctgtggacg agccgcggct cttcgaggtc
     2521 gagccgggcg tccgcctcct ggcgcgctgc cgctggcacg aggatcgact cgcgcggacg
     2581 accctcgtcc tggtgcatgg tctcggcggc agcgccgacg ctccttacat gctgggtacc
     2641 gccctactgg cttacaaagc gggcgcgaac gtggt
//
LOCUS       DASSMB010000191        14739 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_302608, whole genome shotgun sequence.
ACCESSION   DASSMB010000191 DASSMB010000000
VERSION     DASSMB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14739
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302608"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="VFJ72_11425"
     CDS             complement(<1..1113)
                     /locus_tag="VFJ72_11425"
                     /inference="COORDINATES: protein motif:HMM:NF005748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HET7480114.1"
                     /translation="MDSPGSVDLLIANARLLDGQVVDIAVKDGTFYRIGAGLDLSADQ
                     RIEAGGRLVAPPLVDSHVHLDAVLTVGEPRFNQSGTLLEGIQIWGERKKSLTKEDVKR
                     RALEAIKWEVAQGTLFIRSHVDVCDPNLTALKALVELREEVRDIVDLQLVAFPQDGIF
                     AIPEGPRLMERAMELGADVVGGIPHYEFTRDLGEKDVHLAFDLAKRFGRPLDLHTDET
                     DDPNSRFTEIIAANAIRDGLSGKATASHCTAMHSYDNAYAFKLIRLLARAGVNVVANP
                     FDNVVLQGRFDTYPKRRGMARVKELLEAGVNVSLGHDSIMDPWYPLGRGDMLGAASLG
                     LHVLQMSGQSEIDEMLNLVTTRAARTLGIEDRYGIEE"
     gene            1352..2743
                     /locus_tag="VFJ72_11430"
     CDS             1352..2743
                     /locus_tag="VFJ72_11430"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HET7480115.1"
                     /translation="MTTDVINIPEEHGLETHGIERVAPKARVHMRIRDNLTMWLSANL
                     VLSTVSLGALALPIFGLGFWDSVAVVLIFNALGVLPVAFFSTLGPKLGLRQMTISRFS
                     FGWIGAAIMAVFNIAACIGWSAVNVIIGGQLIAAVTNGAIPRWLGILIIAIATTIVSI
                     YGYRYVHRYERYAWIPMAVIFLIIAVVALPHASIIPTPAWNAAHIASFISFGGAIYGF
                     ATGWSSYAADYNVNQPEDTPPGRVFAMTYLGIFSACVLLETLGVALTTIPDLKGKAGG
                     DLLAGAVAPLGGFGTLLVGLLALSVVANNIPNDYSLGLSMQVVSRSFQRVPRWVWTLV
                     GAVAYVAIALPVAADFSLTLEGFLLLIAYWLGPWSIILVLEHFVYRRGVYNVDDWNTR
                     SRLPVGWAAIAAMVAGLFGVVLGASQVYYVGPVAGLINKPFGMDVGFELGIIFAAVAY
                     LILRPRELRASTR"
     gene            2840..4417
                     /locus_tag="VFJ72_11435"
     CDS             2840..4417
                     /locus_tag="VFJ72_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HET7480116.1"
                     /translation="MSRPGFTTILEKDSPPMMFNAGDGFHYEKLPEGTRVIYPPGPVD
                     PLEDPNVAIERALLEPMGMEPLHELLKPGMKLTIVFDDVSCPLPPMKPPDNRQLVIEK
                     VLEKAAAKGVEDIHLISALGLHRRMTEKELRWALGKKIMSRFYPDRLYNYDAEDEEGN
                     VVIGHTKHGEEVRVSRRAAESDLVVYVNINLVSMNGGHKSVHTGISPYSSIRHHHNAE
                     TLRNTRSLMDPKNSALHASVDRMGEILDANVRVFHIETTLNNATFPAVFNFMGQPEHE
                     WNAVTKANFLANHKATQRMPRAVARRIFQSIVAPHRMTSVQAGATHPVHEETIKHLHR
                     QQLVHVEGQSDVLMLGVPYIGPYNVNSVMNPILVHNMVLGYLFNLYRGKPLVRKGGVI
                     IATHPVPNEFDDATHPSYRPYFEEVIAKTTDPDEGERLFQDKYAEDPWYRTLYRSSYA
                     YHGFHPFTTWQWGAHALDHLGDVIFVGGDPYTTARMGYKRADTVAEALQIAQDTVGPN
                     PSKTYMHIPPLFMCEVS"
     gene            complement(4398..5015)
                     /locus_tag="VFJ72_11440"
     CDS             complement(4398..5015)
                     /locus_tag="VFJ72_11440"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET7480117.1"
                     /translation="MRRNLITSRWAALCGIIGPAIFAIDLTALTIAQYGFMINIGWHP
                     LTDPANAWPSGLALGPYGALQDASFAVSGSLLAVFAIGLHRGLRGRSKAGPALLFVSG
                     AAMSLLAFETDPIDRTGPRSPHGLIHDASFVLFVAALLLALLYLSLRMRGDSRWRTHS
                     HYTLGTALGASACLLLGGPAYYLFIAGTLLWFELTAVKLASSPRT"
     gene            5184..5690
                     /locus_tag="VFJ72_11445"
     CDS             5184..5690
                     /locus_tag="VFJ72_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7480118.1"
                     /translation="MTSQAEPQATTGEEGRTTAGTVYVVDPHPLNWLYITWNTMEEPV
                     RTDERGNIVGAAMESSYWEGATLVVKVREGVRFQDGTTLTAHSIRRAFDEVQKWKAPH
                     PPGTYLNFHPDTTVDVTDDYTIRFNFPEPDGLALAKFRGFHIPSESFWTDGGGFGYRK
                     LGSGEGHW"
     gene            5772..6797
                     /locus_tag="VFJ72_11450"
     CDS             5772..6797
                     /locus_tag="VFJ72_11450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7480119.1"
                     /translation="MVSGDPFACTWLFTDFPRSPRVVLEANTDHWNKQRGPRLERVVY
                     VNDIDPAEALEKVCNTEGEIDIVSEVSPADARKVIDSEHANLVSVDALRVVSGLINRN
                     ADFMDDVRVRKALNLAVDRGRVIDEVFGGYAHPVAALAPPYSGGAPDGVEPYPRDPEE
                     AKRLLSEAGWPEGRPLRLAATSDVAAVAERLAGDFRDALGIEVDLISIPDDQLLAAQK
                     TLIEKNLPLEFDVLVHAWFDLAAGYPPAVIHREYFHSLGAFRAGPPIPEFEDLFGRSV
                     VETDGERLTELGKQIDRLVYDEALSVFLCCPMALFAVNKHVRFTGHAATLELAETEVD
                     EQHWSRR"
     gene            6736..7077
                     /locus_tag="VFJ72_11455"
     CDS             6736..7077
                     /locus_tag="VFJ72_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7480120.1"
                     /translation="MPLPWSWRRPRSTSSTGRVDKVLVRVGSRGAVGSLASDSVRRRR
                     EGAGDRPADEQSLGLSTEKLSGMAQPGQPLFGGGIPLMRDGALVGAVGVSGGTVDQDQ
                     EVAEAGLAAFS"
     gene            complement(7154..8017)
                     /gene="fghA"
                     /locus_tag="VFJ72_11460"
     CDS             complement(7154..8017)
                     /gene="fghA"
                     /locus_tag="VFJ72_11460"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HET7480121.1"
                     /translation="MTQDLTTISEHACFGGTTSFHEHLSESCDANMRFAVYAPPQAAE
                     SPVPALYYLAGLTSTEANFVEKSGAQRYAAEHGLMLVAPDTSPRGLGIPGEDDDWDFG
                     SGASFYIDATAEPWSKHYRMYSYVTEELPRIIEANFSASSEVRGIFGHSMGGHGALTI
                     ALKNPDRYRSVSAFAPIAAPTRAPWGEKAFSGYLGTEDKEKWREHDASELVRERPFPD
                     GREILVDQGTGDQFLEEQLYPEVFENACADAGQPLTLRRHEGYDHGYYFISTFMEDHI
                     RHHAKTLNALF"
     gene            complement(8031..9140)
                     /locus_tag="VFJ72_11465"
     CDS             complement(8031..9140)
                     /locus_tag="VFJ72_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HET7480122.1"
                     /translation="MDIKAAVAFGAGEPLKIETVQLEGPKAGEVLVEIKSTGVCHTDE
                     YTRSGADPEGLFPSVLGHEGAGIVVEVGPGVTSVTEGDHVIPLYTPECRECEYCLNPK
                     TNLCQAIRTTQGQGLMPDGTSRLSLNGDPLYHYMGTSTFATHSVVPEIALAKIREDAP
                     FEKVCYIGCGVTTGVGAVVNTAKVEPGSNVVIFGLGGIGLNVVQGAKMVGADKIVGVD
                     INPARKELAEKFGMTHFVNPKEVEGDLVPYLVDLTGGGADYSFECIGNVDVMRQALEC
                     CHKGWGVSVIIGVAGSGEEISTRPFQLVTGRSWRGSAFGGARGRTDVPKIVDWYMEGK
                     LNIDDLITHSMPLEDINTAFDLMHRGESIRSVVNF"
     gene            complement(9239..11335)
                     /locus_tag="VFJ72_11470"
     CDS             complement(9239..11335)
                     /locus_tag="VFJ72_11470"
                     /inference="COORDINATES: protein motif:HMM:NF039646.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HET7480123.1"
                     /translation="MTSRLRASRLWSVEALVVAASLLAALVLGLGRNLPGSFDPVLFV
                     CAAVLFLAPGALVARRFAADLFPGAALVPAAFAISAGLFGILGVPALILHRSLDLYLS
                     ICGTVLAASLAVAAVRAARRKPEIGSKSGAKTASSPTFWLWGPFLILGGVLAWTSRVA
                     RIDGDTWNYLAWVREYLNTRSLALYDPYFGTRTPEFSRVKINGWILEQAALSRVTGID
                     PIPLALQYLGPALILVSLLAVYALARALFQNRGAALLTATIYTLFFLAYLRASQFSIG
                     GELLSRIVQDKDVTRFLFLPVALCFAAAFLEKRRLAHLLLFGFLCWSVVSVHPAGLAV
                     IGLSAAGLGMFHVAAHPRTPEVWTRAVALGLALLSVLLVPLLYVLLTGKALSSALYSA
                     DIGASDPAVLANQVFVQKSWMRIFVLGDGSYIMHPYLVENPVIAASYLLGVPFLIWRL
                     RRSVAARLLLGMLFAATIVCYVPPVATFVGDEIVAPGQLYRLSWPIPLAALLTLGWMA
                     WEAIRRVSERLRLRPGASALLGLALVLALSGAALPSAASGIKEVRAQRDPQGNTGRLD
                     PMFWWMQRNIKEPSVVLANDKANVLIPAYSANANVISFRGQPVLDHLSALERLAGRPI
                     KVRQGSLDMRDFYSGPTFGEAYDILRRNRVDYVLVGAGSTLDSQMKHLPGFTRLDIPS
                     KRYALFKVDRSKLGKP"
     gene            complement(11374..12498)
                     /locus_tag="VFJ72_11475"
     CDS             complement(11374..12498)
                     /locus_tag="VFJ72_11475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480124.1"
                     /translation="MRRLLILTCAIVLVDTIFYAALTPLVPHFKEEFGLSKSAVGVLS
                     GAFGAGVLVGAAPGGYMASRLGIKVTALAGLALMSATSLAFGLADTAPLLVLTRFAGG
                     FGSALSWVAAFTWLVRRAPEERRGELIGVMLSAAVFGALLGPVLGSVAAIVGVFPAFA
                     TVAAAGVGISAWAALEPGPGLVERQSLLGALRTVRQPRLLTGLWFIALSPLIFSALAV
                     LVPLELNRLGWGAAAVGAVFLASAAFEASVHPLLGRWADRSGFRPPILIGLLASILIL
                     IALPFAGSPWLLAGLVVLAAGAFNATLVPGTTLFSRGTEKAGMDQAIAFAITNFAWAA
                     GYAVGAPLGGALADIQGDALSYLVLTIVCALTLLLLRRAS"
     gene            12570..13049
                     /locus_tag="VFJ72_11480"
     CDS             12570..13049
                     /locus_tag="VFJ72_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET7480125.1"
                     /translation="MAYADRASVRRVREALEERGISAEMVELEQTARSAAEAADALGC
                     RVEQIVKSLVFRGSESGGPILVLASGPNRVNEERLSALISEPVEKADAAFVRDKTGYA
                     IGGVPPFGHAAPHLTFVDEDLMLHETVWAAAGHTHVVFGLRPADLLEASGGEVIPVR"
     gene            complement(13082..13426)
                     /locus_tag="VFJ72_11485"
     CDS             complement(13082..13426)
                     /locus_tag="VFJ72_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480126.1"
                     /translation="MMEMHEMGMHGMDGAMGGWGIFGIAASLISLLFLAGLVALILLI
                     ASRVLSGGRGFARTNSAEDILRERFARGEIPAEEYERSMEVLRDNPPHKSYEDYIRDA
                     MNRLRFGRSAGS"
     gene            complement(13615..>14739)
                     /locus_tag="VFJ72_11490"
     CDS             complement(13615..>14739)
                     /locus_tag="VFJ72_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7480127.1"
                     /translation="LGLDLKVISGDDPRTVAALARQAGFPDGIELVSGPELAAMDDEE
                     FDRAAAGATVFGRIIPEQKDRLVDALMRRGLRVAMMGDGVNDVLALKKASLGIAMQSG
                     SGAARDVADMVLLDDSFGVLRPAFHEGRRIIGGMINSLYLFLTRVATSTLIIMAVTMI
                     GLDFPFDPAQVSLTTFSVGVPSFFLTLWARPQRMEEDLLPSLARFVTPAALVGMLMGA
                     GIYVADYNVVLGSPLVSGDIPDKVKQVYESYTGVSFGSEGYRDIVATVTAQGSLSIFV
                     SWIACFVILFLEPPSRLFLGWRKEVSPDKRPALLAAALFVLFFVIWSVPSVGYYFGIL
                     VKPLPITMILLGLAVVWFFLVRTIWRTNLFERVLGLDKVK"
BASE COUNT         2635 a         4722 c         4931 g         2451 t
ORIGIN      
        1 ctcttcgatg ccgtagcggt cttctatgcc taaagttcgg gcggcgcggg tggtgacgag
       61 gttcagcatc tcgtctatct cgctctggcc gctcatctgt aggacgtgca ggccgaggct
      121 ggccgcgccg agcatgtccc cgcgtcccag gggataccag gggtccatga tcgagtcgtg
      181 ccccagagag acgttcacgc ccgcctccag aagctccttg acgcgggcca tgccgcgcct
      241 cttggggtag gtgtcgaagc ggccctggag gacgacgttg tcgaagggat tggcgacgac
      301 gttcacgccg gctcgggcga gtagccggat cagcttgaag gcgtaggcgt tgtcgtagga
      361 gtgcatggcc gtgcagtggc tcgcggtcgc cttgccggag agcccgtcgc ggatggcgtt
      421 cgcggcgatg atttcagtga agcgtgagtt cgggtcgtcg gtctcgtcag tgtgcaggtc
      481 gagcggccgg ccaaaccttt ttgcaaggtc gaaggcgagg tgtacgtcct tctccccgag
      541 atcccgggtg aactcgtagt gggggatgcc gccgaccacg tccgctccaa gctccatcgc
      601 ccgctccatg agccgtggtc cctcggggat ggcgaagatc ccatcctggg ggaaggcgac
      661 gagttgcagg tccacaatgt cgcggacctc ctcgcgcagc tcaacgaggg ctttcagggc
      721 ggtgaggttt gggtcgcaga cgtcaacgtg gctgcggata aagagcgtcc cctgggcgac
      781 ctcccacttt attgcttcca gggcgcggcg tttcacgtct tccttcgtga ggctcttctt
      841 gcgctcgccc cagatctgga tcccttccaa aagcgtcccg ctctggttga agcgcggctc
      901 gcccaccgtc aggacggcgt ccaggtggac gtggctgtcc accagcggcg gcgcgaccag
      961 gcgcccgccg gcctcgatcc gctggtcggc cgagaggtcc agccccgcgc cgatccggta
     1021 gaaggttccg tcttttactg ctatgtccac gacttgccca tcgagcagcc gggcgttggc
     1081 tataaggagg tcgacgcttc cgggagagtc catgaggagg ataatagagt attccgggcc
     1141 gcgcgctcta ccctgcggga ccgtcgcggg tgggatccgg cctcaagttt tggcccgatg
     1201 gagagtagac gggataatct ggtttgcgta aacttcggtt attctgacga ccgcaaaggc
     1261 acgagcaaga ccggtgatgg gctgtgcggc ggtagcggca gtgaggtttg cgtcctgcgg
     1321 agacgcgggc gcggttgctg aggagaggcg catgacgacc gatgtcataa atatccccga
     1381 agagcacggt ctggagacgc acggcatcga gcgcgtcgcg cccaaggccc gggtccacat
     1441 gaggatacgg gacaacctga ccatgtggtt gtcggccaac ctggtgctct cgaccgtctc
     1501 tctgggggcg ctggcgctcc cgatcttcgg gttggggttc tgggacagcg tcgcggttgt
     1561 cttgatcttc aacgccctcg gggtgttgcc ggtggcgttc ttctccaccc tggggcccaa
     1621 gctggggctc cggcagatga ccatctcacg gttctccttc gggtggatcg gggcggccat
     1681 catggcggtg ttcaacatcg ccgcgtgtat cgggtggtcc gccgtcaacg tgatcatcgg
     1741 cggccagctc atcgcggcgg tgacgaacgg ggcgataccg cgctggctcg gcatcctgat
     1801 catagccatc gcgaccacca tcgtcagcat ctacggctac cgctacgtcc accgctacga
     1861 gcggtacgcc tggatcccga tggccgtgat cttcctgatc atcgccgtcg tcgcgctgcc
     1921 gcacgccagt atcatcccga ccccggcttg gaacgccgcg cacattgcct ccttcatctc
     1981 cttcggcggc gcgatctacg gcttcgccac cggctggagc tcctacgccg ccgactacaa
     2041 cgtcaaccag ccggaggaca cccctccggg ccgggtgttc gctatgacgt acctcggcat
     2101 cttctcggcc tgcgtcctgc tcgaaaccct cggcgtagcc ctcaccacca tccccgacct
     2161 gaagggcaag gcgggcggag atctcctggc cggagctgtc gccccgctcg gaggcttcgg
     2221 gacgctgctc gtcggcctgc tcgccctgag cgtggtggcg aacaacatcc ccaacgacta
     2281 cagtctgggc ctctcgatgc aggtggtgag ccggtccttc cagcgcgtgc cgcggtgggt
     2341 gtggacgctc gtcggggccg tcgcctacgt ggcgattgcg ctgccggtgg cggcggactt
     2401 cagcctcact ttggagggct tcctcctgct catcgcctac tggctggggc cgtggtctat
     2461 catcctggtg ctggagcact tcgtgtaccg gcggggcgtc tacaacgtgg acgactggaa
     2521 cacccgttcc aggttgcccg ttggatgggc cgcgattgca gcgatggtcg cggggctctt
     2581 cggggtcgtc ctgggagcgt cgcaggtgta ttacgtgggg ccggtggcgg gtctgatcaa
     2641 caagcccttc ggcatggacg tggggttcga gctgggcata atcttcgccg ccgtcgcgta
     2701 cctgatcctg cgtcccaggg agttgcgcgc ttccacccgc tgaaccggcg ttcgcggcgc
     2761 tcccggagcg ggtcaaccgg cggctgatcg agtaaagtat ccgaaaagcc ggttacagac
     2821 cccacgggag gaagagtaga tgagccgtcc cggtttcacg accatactgg agaaagatag
     2881 cccgccgatg atgttcaacg cgggcgacgg cttccactac gagaagctgc cggaggggac
     2941 gcgcgtgatc tatcctcccg gtcccgtgga cccgctcgaa gacccgaacg tcgccatcga
     3001 acgggcgcta ctggagccga tgggcatgga gccgctgcac gagcttctga agcccggaat
     3061 gaagctcacc atcgtcttcg acgacgtctc gtgcccgctg ccgccgatga agccgccgga
     3121 caaccgccag ctcgtgatag agaaggtgct tgagaaggct gccgcgaagg gtgtggagga
     3181 catacacctg atctccgcgc tgggcctgca ccgccggatg acggagaaag agctgcggtg
     3241 ggcgctgggg aagaagatca tgtccaggtt ctaccccgac cgcctctaca actacgacgc
     3301 cgaggatgaa gagggcaacg tagtcatcgg gcacacgaaa cacggcgagg aggtgcgcgt
     3361 cagccgccgc gccgccgaga gtgacctggt cgtgtacgtg aacatcaacc tcgtttcgat
     3421 gaacggcggc cacaagtccg tccacaccgg catctcgccc tactcctcca tccgccacca
     3481 ccacaacgcc gagacgctaa ggaacacccg ctcgctgatg gacccgaaga actcggcgct
     3541 gcacgcctcg gtggaccgga tgggcgagat cctggacgca aacgtcaggg tcttccacat
     3601 cgagaccacc ctgaacaacg cgactttccc ggcggtcttc aacttcatgg gccagccgga
     3661 gcacgagtgg aacgccgtga cgaaggcgaa ctttctcgcc aaccacaagg ccacgcagag
     3721 gatgccgcga gccgtggcgc gcaggatctt ccagtccatc gtcgccccgc acaggatgac
     3781 gagcgtccag gcgggcgcga cgcacccggt gcacgaggag acgatcaaac acctccaccg
     3841 ccagcaactc gtccacgttg agggccagtc cgacgtcctg atgctcggcg ttccgtacat
     3901 cggcccgtac aacgtcaact ccgtaatgaa cccgatactg gtccacaaca tggtgctcgg
     3961 gtacctcttt aacctctaca gggggaagcc gctcgtgagg aagggcggcg ttatcatagc
     4021 gacccatccc gtccccaacg agttcgacga cgctactcac ccgagctacc ggccgtactt
     4081 cgaggaggtg atcgccaaga cgaccgaccc ggacgagggc gagcgtttgt tccaggacaa
     4141 gtacgccgaa gacccctggt accggaccct ctaccgcagc tcctacgcct accacggctt
     4201 ccacccgttc acgacctggc agtggggcgc gcacgccctc gaccacctcg gggacgtgat
     4261 cttcgtcggc ggcgacccgt acaccaccgc gaggatgggc tacaagcgcg ccgacaccgt
     4321 cgccgaagcc ctccagatcg cccaggacac cgtcggcccg aacccctcca agacctacat
     4381 gcacatcccg ccgctcttca tgtgcgaggt gagctagcga gcttcaccgc cgtcagctcg
     4441 aaccacagca gggtcccggc gatgaacagg tagtaggccg gtccgccgag gaggaggcag
     4501 gcggatgcgc caagcgcggt tccgagtgtg tagtgggagt gcgtacgcca tcttgaatcg
     4561 ccgcgcatcc ggagagagag gtacaacaga gcaaggagca gcgcggccac gaagaggacg
     4621 aacgaggcgt cgtggatgag gccgtgcggg gagcgggggc cggtgcggtc tatcgggtcg
     4681 gtctcgaagg cgaggagcga catcgcggcg ccggagacga agagtagcgc cggtcccgcc
     4741 ttcgagcgcc cgcgcaagcc ccggtgcagg ccgatggcga agacggcgag cagggagccg
     4801 gagacggcga agctcgcgtc ctggagggct ccgtagggac cgagggcgag gccgctcggc
     4861 caggcgttcg ccggatcggt gagcggatgc catccgatat tgatcatgaa cccgtactgc
     4921 gcgatcgtca gggccgtcag gtctatggcg aagatcgccg gaccgatgat gccgcagagg
     4981 gccgcccatc gtgacgtaat gagatttctt cgcatcagtg ttctcctgta ccgcgtacat
     5041 gcttgcatca cgcaagggac tccacaacac agactttttc gatcaccggg gtgatctatt
     5101 cagcgccggc ttccgttgtg gttgtggggt atgtaattct agtagtattc ccgcgtcgaa
     5161 gggacaaatt ggaagggaga ggcatgacga gtcaggccga gccgcaagct accacgggag
     5221 aggagggacg aacgacggca ggcacggtct acgtcgtgga cccgcatcct ctgaactggt
     5281 tatacattac ctggaacacg atggaggagc cggtccgcac cgacgagcgg gggaacatcg
     5341 ttggtgcggc gatggagagc tcctactggg agggcgcgac cctggtggtg aaggtccgcg
     5401 agggcgttcg ttttcaggac ggcaccacgc tgacggcgca cagcatccgg cgcgcttttg
     5461 atgaggtaca gaagtggaag gcgccccatc cacctgggac gtacctgaac ttccacccgg
     5521 acaccacggt ggacgttacc gacgactaca ccatccgctt caacttcccc gagccggacg
     5581 ggctcgccct ggcgaagttc cgaggcttcc acatcccgag cgagagcttc tggaccgacg
     5641 gaggggggtt cggatacaga aagctgggga gcggcgaagg acactggtga gcgatcgacg
     5701 ctccgggccc gtggggctcg ggaccgttca ccctcgtcga gggctactcc accatcgaga
     5761 acgtgatcgc catggtctcc ggcgacccgt ttgcctgcac ctggctgttc accgacttcc
     5821 cgcggtcgcc ccgcgtggtg ctggaggcga acaccgatca ctggaacaag cagcgcggcc
     5881 cgcgcctgga gagggtcgtc tacgtcaacg acatagaccc cgccgaggcg ctggagaaag
     5941 tctgcaacac cgaaggtgag atcgacatcg tctccgaagt ctcgcccgcc gacgctcgga
     6001 aggtcatcga ctccgagcac gccaacctgg tgagcgtgga cgccctgcgc gtcgtctccg
     6061 gactgatcaa ccgcaacgcg gattttatgg acgacgtgcg tgtccgcaag gcgctcaacc
     6121 tggccgtgga ccgcggccgc gtgatagacg aagttttcgg gggctacgcg cacccggtcg
     6181 ccgcgctcgc gcctccgtac tccggcggtg cgcccgacgg ggtggaaccc tacccgcgcg
     6241 acccggagga ggcaaaacgg cttctctcag aggctggctg gcccgaggga cggcctcttc
     6301 ggctcgccgc caccagcgac gtcgcggccg tggcggagcg cctcgccggc gacttccgcg
     6361 atgccctggg gatcgaggta gacctgatct ccatccccga cgaccagctt ctcgcggccc
     6421 agaagaccct gatcgagaag aacctgccgc tagagttcga cgtgctcgtg cacgcctggt
     6481 tcgacctggc cgcgggctac ccgccggcgg tcatccaccg cgagtacttc cactctctcg
     6541 gcgccttccg cgccggtccg ccgatcccgg agttcgagga tctattcggt cgctctgtcg
     6601 tggagaccga cggagagcgg ctgacggagc tgggcaagca gatcgacagg ctcgtctacg
     6661 acgaggctct gagcgttttc ctgtgttgcc cgatggcgct cttcgcggtc aacaagcacg
     6721 tcaggttcac cggccatgcc gctaccctgg agttggcgga gaccgaggtc gacgagcagc
     6781 actggtcgcg tagataaggt tctagttcga gtcgggagtc ggggagcggt tggttctctg
     6841 gcttcagact ctgtacgccg ccgaagagaa ggcgcgggag atcggccagc cgatgaacag
     6901 tccctcggtc tgtccacgga gaagctgtcc gggatggcgc agcccggaca gccgctcttc
     6961 ggcggtggta tcccgctcat gcgcgatggc gcgctcgtcg gcgccgtggg cgtgagcggc
     7021 ggcacggtcg accaggacca ggaggtagcc gaggcgggac tggcggcctt cagctaaacg
     7081 gatcgcaaat cggaaaagga gggtggcgca gactctggtc tgcgccgccc tccatgcggc
     7141 gctttaccac aagctaaaat agggcgttca gcgtcttcgc gtgatgccgg atgtggtcct
     7201 ccatgaacgt cgagatgaag tagtagccgt ggtcgtagcc ctcgtgccgg cggagggtca
     7261 gtggttgtcc ggcgtcggcg cacgcattct cgaagacctc gggatacagt tgctcctcca
     7321 ggaactggtc tccggtgccc tgatccacga gtatctcccg accgtcgggg aacggacgct
     7381 cgcgcaccaa ctcgctcgcg tcgtgctcgc gccacttctc tttgtcttcc gtccccaaat
     7441 aaccggagaa cgccttctca ccccagggag cgcgggtggg cgcggctatc ggggcgaagg
     7501 cggagacgct tctgtaacgg tccgggtttt tcagggcgat ggtgagcgcg ccgtgaccgc
     7561 ccatcgagtg gccgaagatg ccgcggacct ccgaactggc ggagaagttg gcttcgatga
     7621 tccgtggtag ctcctccgtg acgtagctgt acatcctgta gtgctttgac cagggctcgg
     7681 ctgttgcgtc gatgtagaag ctcgcgccgg agccgaagtc ccagtcgtcg tcctcgccgg
     7741 gtatccccag gccgcggggg ctggtgtccg gggcgacgag catcaggccg tgctcggcgg
     7801 cgtaccgctg ggcgccgctc ttctccacga agttcgcctc ggtggaggtc aggcccgcca
     7861 ggtagtagag cgcaggcacc ggactctccg ctgcctgcgg cggcgcgtag acggcgaagc
     7921 gcatgttcgc gtcgcaggac tccgagaggt gctcgtggaa gctcgtcgtc ccgccgaagc
     7981 aggcgtgctc gctaatggta gtgaggtctt gagtcacaag gcattcccct ctagaagttg
     8041 acgacgctgc ggatagactc gccccggtgc atcaggtcga aggcggtgtt tatgtcttcg
     8101 aggggcatgg agtgggttat gaggtcgtcg atgttcagct tgccttccat gtaccagtca
     8161 actatcttcg gcacgtccgt ccggcctcgc gctcctccga aggcgctgcc ccgccagcta
     8221 cggcccgtga cgagctggaa gggacgggtg gagatctcct ccccagaccc cgcgaccccg
     8281 atgataaccg agacgcccca gcccttgtgg cagcactcca gagcctgacg catcacgtcc
     8341 acgttcccga tgcactcgaa cgagtagtcc gcgccgccgc cggtcaggtc cacgagatac
     8401 ggcaccaggt ccccctcgac ctccttcggg ttgacgaagt gggtcatccc gaacttctcg
     8461 gcgagttcct tgcgggcggg gttgatgtcg acgcccacga tcttgtccgc cccgaccatc
     8521 ttcgcgccct gaacgacgtt gaggccgatg ccgccgagtc cgaagatcac gacattcgag
     8581 cctggctcga ccttcgcggt gttgactacc gctccgaccc cggtggtgac gccgcagccg
     8641 atgtagcaga ccttctcgaa cggggcgtcc tcccggatct tcgccagcgc gatctccggt
     8701 acgacgctgt gcgtggcgaa ggtcgaggtc cccatgtaat ggtagagcgg gtcgccattc
     8761 agggagaggc ggctcgtgcc gtcgggcatc aggccctggc cctgggtcgt ccggatagcc
     8821 tggcagaggt tcgtcttcgg gttcaggcaa tactcgcact cccggcactc gggggtgtag
     8881 agcgggatca cgtgatcccc ttccgtcacg ctggttacgc ccggtccgac ctcgaccacg
     8941 atgcctgctc cctcgtggcc caagaccgac gggaagagac cctctggatc tgcgccgcta
     9001 cgcgtgtact catccgtgtg gcacacgccg gtggatttga tctcgaccag cacctcgccg
     9061 gcctttggtc cctcaagctg caccgtttcg atcttcaggg gctctcccgc cccaaatgcc
     9121 acagccgcct taatgtccaa agctcctcct cgaaggtctg tatcccggcc catactctaa
     9181 cgaaacaccc ccacgtttgc cgcacgacca cggcgagagc acgatgggtg gctgcatctc
     9241 acggtttgcc taatttgctg cggtccactt tgaagagggc gtatcttttg ctggggatgt
     9301 ctagccgggt gaaccccggc aggtgcttca tctgactgtc cagcgtgctg ccggccccga
     9361 cgagcacgta gtccacccgg ttgcgacgca ggatgtcgta agcctctccg aaggtcggtc
     9421 cggagtagaa gtcgcgcatg tcgagagagc cctggcgcac ttttatcggc ctgcccgcga
     9481 gccgctccag cgcggacaga tggtccagga ccggttggcc gcggaagctg atgacgttgg
     9541 cgttcgcgga gtaggctgga ataaggacgt tcgccttgtc gttcgccagc accacgctcg
     9601 gctcctttat gttgcgctgc atccaccaga acatgggatc gaggcggccg gtgttgccct
     9661 gcgggtctct ctgcgcccgg acctccttta tccccgaagc cgcggacggc agggccgcgc
     9721 ccgatagcgc gagcaccaac gccaagccaa ggagcgcgga cgcgccgggt ctgagccgga
     9781 gcctctccga gacgcgccgg atcgcctccc aggccatcca gcccagggtg agcagcgccg
     9841 cgagaggtat gggccaggag agacggtaga gctggccggg agcgacgatc tcgtcgccga
     9901 cgaacgtggc gaccggcggg acgtagcaca ctatggtcgc tgcgaacagc attcccagga
     9961 gcagccgggc cgcgaccgag cgccgcaggc gccagatcag gaacggcacg cccaggagat
    10021 aggacgcggc gatgacgggg ttctcgacca ggtaggggtg cattatgtac gagccgtccc
    10081 ccagcacgaa gatgcgcatc caactcttct ggacgaacac ctggttggcg aggacggcgg
    10141 gatcgctggc cccgatgtcc gccgagtaca gtgccgagga gagggccttc ccggttagga
    10201 ggacgtaaag tagagggacc agcaggacgc tgagtagcgc cagccccagc gcgaccgcgc
    10261 gtgtccagac ttcgggtgtg cgcgggtgcg ccgcgacgtg gaacataccg agaccggccg
    10321 ccgacagacc gatgaccgcc agccccgccg gatgtacgct caccaccgac cagcacagga
    10381 agccgaagag caggaggtgc gcgagccggc gtttttcgag gaacgcggcg gcgaagcaaa
    10441 gcgctaccgg caggaagaga aagcgggtta cgtccttgtc ctggactatg cggctcagga
    10501 gttcgccgcc gatggagaac tgcgacgcgc gcaggtaggc caggaagaac agagtgtaga
    10561 tggtggcggt caggagcgcc gctccccggt tctggaagag cgcgcgggcc agagcgtaga
    10621 cggcgaggag agagaccagg atcagggccg gacccaggta ctgaagggcg agcggaatcg
    10681 ggtctatccc cgtgacgcgg gagagggcgg cctgctccag gatccagccg ttaatcttca
    10741 cccgcgagaa ctcgggcgtc cgggtgccga agtagggatc gtagagggcg aggctgcgcg
    10801 tgttcaggta ctcgcggacc caggcgagat agttccaggt gtcgccgtcg atccgcgcca
    10861 ctctggatgt ccaggccagg acccccccga ggatcaggaa cggaccccag agccagaagg
    10921 tggggctgct tgcggtcttt gccccgcttt tgcttccgat ctccggtttt cgccgcgcgg
    10981 cgcgcaccgc ggcgaccgcc agcgacgccg ccaggacggt tccacagata gacagataga
    11041 ggtcgaggct gcggtgcagg atgagggcgg ggactcccag gatgccgaat agtcccgcgc
    11101 ttatcgcgaa cgccgcaggc acgagcgcgg cgccggggaa gaggtctgcc gcgaaccggc
    11161 gcgctaccaa agcgccgggg gcgaggaaca ggacggcggc gcacacgaag aggaccgggt
    11221 cgaaggagcc gggaaggttt cgcccgagtc ccagcacgag cgctgccaaa aggctcgccg
    11281 caacgaccag cgcctcgaca gaccagagac gggaagcgcg taggcgggag gtcaaggccg
    11341 aacttctatc cgagagcctg tgtcgtacgg ttttcaggac gctctcctga gcaggagcag
    11401 ggtcagggcg cagacgatgg tcaggaccag gtacgagagc gcgtcgccct gtatatcggc
    11461 cagagcgccg cccaggggcg cgccgacggc gtagcctgcc gcccacgcga agttggtgat
    11521 ggcgaaggct atcgcctggt ccatccccgc cttctccgta ccgcgagaga agagcgtcgt
    11581 gccgggtacg agcgtcgcgt tgaaagctcc ggcggcgagc acgacgaggc ccgccagaag
    11641 ccacgggctt ccggcgaacg gcagggcgat gaggatcagt atcgaggcga ggagaccgat
    11701 caggatcggc ggtcgaaagc cggagcggtc ggcccagcgt ccgagcagcg gatggacgct
    11761 ggcctcgaag gcggcgctcg cgaggaagac cgcgccgacc gccgccgcgc cccatccgag
    11821 ccggttcagt tcgaggggca cgaggacggc gagggcggag aagatcaggg gcgagagcgc
    11881 gatgaaccag agcccggtca ggagccgcgg ctgccgcacc gtacgcagcg cgccgagcag
    11941 ggactgccgc tcgacgagcc ccggacccgg ctccagggcg gcccaggccg agatcccgac
    12001 tcccgctgct gcgacggttg cgaaggcggg gaagacgccg actatcgccg cgacgctccc
    12061 gagcaccgga ccgagcagcg ccccgaagac ggcggcgctg agcatcaccc cgatcaactc
    12121 tccccgacgc tcctccggcg cgcgccgcac gagccaggtg aaggccgcga cccacgagag
    12181 cgcgctcccg aacccgccgg cgaaccgggt cagcaccagc aggggcgcgg tatccgccag
    12241 cccgaaagcg aggctcgtgg ccgacatcag ggcgagcccg gcaagcgccg tcaccttgat
    12301 ccctagtcgg gaggccatgt agccgccggg agccgcgcct acgaggaccc cggccccaaa
    12361 cgccccgctc aggacgccga ctgcggactt ggagagaccg aactcctcct tgaagtgcgg
    12421 caccagcggc gtcagggcag cgtagaagat cgtgtcgacg aggactatcg cgcaggtgag
    12481 gatcaggaga cgacgcacgg cggttattct agcgcctcgc tacaatgcgc ggggtgtcct
    12541 ggaacatcca agagcaggag ggtcggaaga tggcttacgc ggatagggcg agcgtacgga
    12601 gggtgcgcga agcgctggag gaacgcggca tctccgcgga gatggtggag ctcgaacaga
    12661 ccgcccggag cgcggcggag gccgcggacg ctttaggctg ccgggtggag cagatcgtaa
    12721 agtccctggt cttccggggt agcgagagcg gaggtcctat cctggtcctc gccagcggcc
    12781 cgaaccgggt gaacgaggag cgcctttcag cgctaatctc agagccggtc gagaaggccg
    12841 acgccgcgtt cgtgcgcgat aagaccggat acgcgatcgg cggcgtgccc ccgttcggac
    12901 acgccgcgcc gcatcttacg ttcgtggacg aggacctcat gctgcacgag acggtctggg
    12961 ccgcggcggg tcacacgcac gtcgtctttg ggctccgtcc cgccgatcta ctggaggctt
    13021 ccggcggaga ggtgatcccg gtgaggtgag ccggccttcc ggccaggcgg gacccgcgct
    13081 gtcaggagcc cgcgctgcgt ccgaacctga gccggttcat cgcgtcgcgg atgtagtcct
    13141 cgtagctctt gtgcggcggg ttatcccgca ggacctccat ggacctctcg tactcctcgg
    13201 cggggatctc gccgcgggcg aaccgctccc ggagtatgtc ctccgcagag ttcgtcctgg
    13261 cgaagcctcg gcctccggag agaacccgtg aggcgatgag gaggatcagg gctaccagcc
    13321 ccgccagaaa gagcaggctt atgagggacg cggctatccc gaagatgccc catcctccca
    13381 ttgctccgtc catcccatgc atccccattt cgtgcatttc catcatctgc tcccttctcg
    13441 ccctactcgt gaatgaccgg tgtcggacgc gcgctctccg cgttgcattc gggatcctcc
    13501 cgcgtctctt tcgtgtcttg tcagtagtct ggacctcgaa cctgtgattc gcctgtattt
    13561 agtctatgaa ttttcattgg aaggtcgtgg aggagcctgg cttccccggc atcgttactt
    13621 gactttatcc aggcccagga cgcgctcgaa caggttcgtc cgccagatag tccggacgag
    13681 gaagaaccag acaaccgcca gtcccaggag gatcatggtt atcggcagag gtttcaccag
    13741 gatgccgaag tagtacccga ccgagggtac cgaccagatg acgaagaaca acacgaagag
    13801 cgcagcggcc aggagggccg gtcgcttgtc cgggctcacc tccttgcgcc agccgaggaa
    13861 gaggcggctg ggcggttcca aaaacaggat gacgaagcag gcgatccagg agacgaagat
    13921 ggagaggctg ccctgagccg tgacggtagc gacgatgtcc ctgtaaccct cgctcccgaa
    13981 ggagaccccg gtgtacgact cgtacacctg cttcaccttg tcggggatgt cgccgctcac
    14041 cagggggctg ccgagcacca cgttgtaatc ggccacgtag atgcccgcgc ccatgagcat
    14101 gcccacgagg gcggcggggg tgacgaagcg cgccagggac ggcaagaggt cctcctccat
    14161 ccgttggggg cgcgcccaca gcgtcaggaa gaacgaaggc acgcccacgc tgaacgtggt
    14221 cagggagacc tgggccgggt cgaaggggaa gtccagcccg atcattgtta ccgccatgat
    14281 gatcagggtg ctcgtcgcca cgcgggtcag gaagaggtac agcgagttga tcatgccgcc
    14341 gatgatgcgc cgcccctcgt ggaacgccgg gcgcaacacg ccgaaggagt cgtcgagcaa
    14401 gaccatgtcg gccacgtccc gcgccgctcc gctgccgctc tgcatcgcga tccccagcga
    14461 agcttttttg agcgccagca cgtcgttgac gccatctccc atcatcgcga cccgcaggcc
    14521 ccggcgcatc agggcgtcca ccagccggtc tttctgctcg gggatgatgc gtccgaagac
    14581 cgtagccccg gcggccgccc ggtcgaactc ttcgtcatcc atcgccgcca gctccggccc
    14641 ggagaccagc tctatgccgt ccgggaaccc ggcctggcgg gccagggcgg cgacggtgcg
    14701 cgggtcgtcg ccggagatga ccttcagatc caggccaag
//
LOCUS       DASSMB010000192        10886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_302796, whole genome shotgun sequence.
ACCESSION   DASSMB010000192 DASSMB010000000
VERSION     DASSMB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302796"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1189
                     /locus_tag="VFJ72_11495"
     CDS             47..1189
                     /locus_tag="VFJ72_11495"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET7480128.1"
                     /translation="MKFSTRAIHVGQEADPATGATIVPIYQTSTYTQEGPGQHKGYEY
                     SRTGNPTRTALEKCVAALEDAEHGLAFASGLAATTAVMSLLSPGDHVVAGDDLYGGSY
                     RLFDKVLQKSNGLDFTFVDTSDLASVENALRPETKMLWIETPTNPMLTLSDIQALSEM
                     AHERGAIVAVDNTFASPYFQQPLSLGADIVVHSTTKYMGGHSDVVGGAAVTSDTDLHE
                     RMAFYQNAAGAVPGPFDSWIVLRGLKTLAVRMRQHEQNALAVAEFLKGHPEVETVNYP
                     GLPSHPQHELAKKQMSGFSGMVSFTLKGGAEAAYAAVKKTRIFSFAESLGGVESLITH
                     PATMTHAAIPREQREARGVTDGLMRLSVGIEDAEDLIADLDRAISS"
     gene            complement(1186..1977)
                     /locus_tag="VFJ72_11500"
     CDS             complement(1186..1977)
                     /locus_tag="VFJ72_11500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7480129.1"
                     /translation="MTVRVGAQIQPQHAEYKQMRDTWLRVEEMGADTLFNWDHFYPLY
                     GEPEGKHFECWTLLGAMAEVTERVEFGALVTCNSYRNPNLLADMARTVDHISGGRLIL
                     GIGSGWFEKDYDEYGYEFGTAPGRLKKLDAALPVIEERFGKLNPAPTREIPILIGGGG
                     EKVTLRIVAEHAHIWNGFGDPEAAGRKSRILDDWCEKVGRDPKEIERSILIGPEQVKN
                     GDAYAENGITHLILRAGGPDYDISALKDLVAWRDDYNERNGEAVA"
     gene            complement(2032..2346)
                     /locus_tag="VFJ72_11505"
     CDS             complement(2032..2346)
                     /locus_tag="VFJ72_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480130.1"
                     /translation="MTDRDGGTRTTFESTTGGVMTEEAGAVTGELELSTEVGEGLRAS
                     VRYAGAEEWYTVEGSPIPLKAGAGEDPTELHRRVEECLKTPGPVVDANERAATLKWFS
                     VV"
     gene            complement(2350..3030)
                     /locus_tag="VFJ72_11510"
     CDS             complement(2350..3030)
                     /locus_tag="VFJ72_11510"
                     /inference="COORDINATES: protein motif:HMM:NF019151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HET7480131.1"
                     /translation="MSSKSIVRLIVVLLILAVGGTGVFSELDNTGGGSGGGKVDTGAA
                     PASVVKTLDGLTVSPPGSMAGYSREKFPHWSDAQESGWDADPSCDARDAALIRDGKDV
                     QVGSGCDVISGKWLDPYTTRTYTEPLDIDIDHVVPLANAWRSGASGWDTAERERYAND
                     PDVLLSVEDNANQEKGDKGPEAWKPPNKGEWCDYAKRWVQIKDKYDLSVNEQEKQSLV
                     QMLNGCGG"
     gene            3125..3856
                     /locus_tag="VFJ72_11515"
     CDS             3125..3856
                     /locus_tag="VFJ72_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET7480132.1"
                     /translation="MTSPDIDVGDIRALAFDVFGTVVDWRSTIIREGEELGREKSLGI
                     DWAAFADAWRGRYAPSMDRVRRGEMPWTNLDALHRASLEELLTEFGIESLTEAERDRL
                     NRVWHRLAPWPDSVEGLMRLKESYIITTLSNGNVALLTNMAKRAGLPWDLVLSAEIVR
                     HYKPDPETYLMVPELLDLRPDQVMLVAAHPDDLRAARDNGLGTAYVLRPREWGPDGEA
                     EAPDPSFDLVAEDFLDLARKLEALR"
     gene            complement(3849..5084)
                     /locus_tag="VFJ72_11520"
     CDS             complement(3849..5084)
                     /locus_tag="VFJ72_11520"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480133.1"
                     /translation="MKRAEVHVAGNLAFAVLLISQLAATAGFMFVMPFMPLYVQRLGV
                     EDAGSAAAWAGVLNTATALTMALAAPMWGRLADRLGPKTMLMRAAFAGSIVVGLMGLA
                     TSPWHLLVLRLVQGTLTGTVAAATVLASATAPQGKAGARLGTLQMIIFVAAAAGPFVG
                     GIFAEAVGIRASFGVTAALLFLSGVLVLFGVEGAESAPEEEEVRESGPLPWRALLIGL
                     IPLFFVNVAITGVAPALPGFVGELAGAGAQVAGLSGQILGAGALAAAVGSLVGGGLAA
                     RFGTMKVMVLALLLAGLASLPQAAVTSVAELFGLRIIISFALGLVIPVANLAVRSAVA
                     SGRQGEAFGVAASVNSAAFGLGPLGGGLLAASVGFEAPFIAPGILLLVASVALLGLAL
                     ASRPRYVRLLRLVFANLVT"
     gene            complement(5231..5968)
                     /locus_tag="VFJ72_11525"
     CDS             complement(5231..5968)
                     /locus_tag="VFJ72_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7480134.1"
                     /translation="MSAALLVALVAAFLAGTVSGLTGFGLALISTPLLLFVYEPRTVI
                     VLSAILSIFINLAVVWDSWREAHRRLVVSLFLPALVGIVAGAEVLRAVDAVYIRLGVG
                     IVVVISALLLVREVRLPGADTRWGTLVAGTASGALSTSTGLAGPPIVLLLASRGLPKH
                     DFRGTSALYFLFTSIAGIAVLSLRGLVDAGQLPLALALIPASFLGKALGTSFLKRFSE
                     NAFRVLTLVIVILTGALGVATAVWALL"
     gene            complement(6043..7104)
                     /locus_tag="VFJ72_11530"
     CDS             complement(6043..7104)
                     /locus_tag="VFJ72_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HET7480135.1"
                     /translation="MVSTTTGSRMMDSTDKDILNFIQREFPLEREPFAAIGREVGLAG
                     DEVIRRIEMLKRGRVIRQISAIFDTRVLGYQSTLVAAKIPKEKLATGAKAINSHPGVS
                     HNYERNNELNLWYTVAVPPDSRLGLEGTVDVLHHISGAEKTRILPTLKLFKIGVTLDM
                     NEGATAKKEAPQYGESDRQGADRNISEEDKAAIRALQEDVPLTPRPFDLWGEQAGMSY
                     GELLQRAFDLQRRKIMRRFSAVLYHRKAGFRANAMGVWKVPDERIEEVGNMFAQYQAV
                     SHCYQRPVYEDWPYALFSMVHGRSVEECQAVLDAMSEESGLADYASLYSTREYKKTRV
                     RYFTPEMEAWERLYAGVLR"
     gene            complement(7212..7856)
                     /locus_tag="VFJ72_11535"
     CDS             complement(7212..7856)
                     /locus_tag="VFJ72_11535"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7480136.1"
                     /translation="MDSLFDSAENGESREQRLAELSRQVSVCTKCDLAMSRTNTVFGT
                     GDPHSPLMLIGEGPGENEDATGLPFVGRAGKLLDDILKAVDLTRDDVYITNIVKCRAA
                     VQEGGRMKNRPPKTGEIRACNPYLEAQIDAVKPEIILCLGGPAAKTMIDKDFRITKDR
                     GKWYEVHGAKAMATFHPAYVLRQSGDDLKATKRLVWKDVQAVYAEYQKAMEERG"
     gene            7940..8671
                     /locus_tag="VFJ72_11540"
     CDS             7940..8671
                     /locus_tag="VFJ72_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7480137.1"
                     /translation="MSIIEFHAVNKFFGDFHVLKDIEFSVDEGEVVVVIGPSGSGKST
                     LLRCINALEEITTGELIVDGIRVHDKKTDLNKLRTEIGMVFQQFNLYPHMTVAENIKL
                     APTKVKGVSSSEANDRCKRLLERVGIPEQANKYPISLSGGQQQRVAIARGLAMEPKIM
                     LFDEPTSALDPEMINEVLEAMADLAKGGMTMVVVTHEMGFARRVADRVVFMDEGRIVE
                     VGPPEHFFQNPENERTKRFLAQILH"
     gene            8689..9744
                     /locus_tag="VFJ72_11545"
     CDS             8689..9744
                     /locus_tag="VFJ72_11545"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7480138.1"
                     /translation="MKPRSALDAIRPYHPPRMEWEIAADRGANEYVKLTMNELSFGPL
                     PEAKAAALEALARGNRYPERDSDPLRQAIAEANPGVAPDNVFIANGSSEVLVDLMQTL
                     ERPGEVIFPWPSFPLYSSAARIVGLAERRVPLDEHHAVDLDALLAAVGPETRAVLLCN
                     PNNPTGTHLPLSEVRAFARALPEEVLLILDEAYYEFVTDPAYPGSHELALESENVVTT
                     RTFSKVHGLAGLRVGYVIAPPKVADYAGRAHTPFSVNLIAQAAAEASMRATESIAERA
                     RFMAGERDRVQAAFDAAGLEYIPSQTNFILARIGPEVFEKAGVLVREGEALGCPGWSR
                     ISLGDSGENDRLLSALS"
     gene            complement(9752..>10886)
                     /locus_tag="VFJ72_11550"
     CDS             complement(9752..>10886)
                     /locus_tag="VFJ72_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HET7480139.1"
                     /translation="HAFRERGLDVDVWEATREEMEPYLEHVGEQETYAGRPNVVGRRA
                     GSGGGRSILLNAHIDTVDPGDPAAWTRDPAGELDGNLLYGRGACDMKGGLVTHLLALD
                     ALADLGIELRGDVKVAATVGEENGGLGALSTVLRGHRADAALITEPTRLALVPAQGGS
                     LVFRLTIHGRSAHAAVRDAGVSALEKFVPIFEDLRALERERNATLSHPLYDGMKNKVP
                     INVGLVRTGNWASTVPETLVAEGRIGLIPGEEVEPFRQQVVERILAVAGRDPWLRENP
                     PEVEWFGGQFAPAEVPPEAPICQAVARAHELTTGRKPSIEGVPYGADMRLFIRFGEMP
                     CVMYGAGDVNAAHAPDEHISIPDLLTAAKTVALLLADWCGVEE"
BASE COUNT         1872 a         3763 c         3459 g         1792 t
ORIGIN      
        1 aaggaagacc tcgaaaaacc gtaccggatc ttcggggagg gatgatatga agttctccac
       61 gcgagccatc cacgtcgggc aagaggcaga cccggcgaca ggcgccacga tagtcccgat
      121 ctaccagacc tccacctaca ctcaggaagg tcccggccag cacaagggct acgagtacag
      181 ccgcaccggc aacccgaccc gcacagccct ggagaagtgc gtcgccgccc tcgaagacgc
      241 cgagcacggt ctcgcctttg cctccgggct cgccgccacg accgccgtga tgagcctgct
      301 ctctccagga gatcacgtcg tcgccggaga tgacctctac ggcggcagct accgactctt
      361 cgacaaggtc ctccagaaga gcaacggcct cgacttcacc ttcgtggata cgagcgacct
      421 ggcatccgtg gagaatgcgc tgcggccgga gacgaaaatg ctctggatcg agactcccac
      481 caatccgatg ctcaccctct cggacataca ggcgctctcc gagatggcgc acgagcgcgg
      541 ggcgatagtc gccgtggaca acaccttcgc ctcgccgtac ttccagcagc cgctctctct
      601 aggcgcggac atcgtggtcc actcgacgac gaagtacatg ggcgggcact cggacgtggt
      661 cggcggggcg gccgtcacct ccgacacgga cctgcacgag cggatggcct tctaccagaa
      721 cgccgccggg gcggttccgg gaccgttcga ctcctggatc gtgctccggg gcctcaagac
      781 cctcgccgtg aggatgcgcc agcacgagca gaacgcactc gccgtcgcgg agttcctgaa
      841 gggccacccc gaggtcgaga ccgtcaacta ccccggcttg ccgagtcatc cgcagcacga
      901 gctggcgaag aagcagatga gcggcttctc cgggatggtc tcctttaccc tcaagggcgg
      961 cgcggaggcg gcctacgcgg cggtcaagaa gaccaggata ttctccttcg ccgagtctct
     1021 gggcggcgtc gagtcgctca tcactcaccc ggccacgatg acccacgcgg ccatcccgcg
     1081 cgagcagcgg gaggcgcggg gagtgacgga cgggctaatg cgcctctccg tcggcatcga
     1141 ggacgcggag gacctcatcg cggacttgga ccgggctatc agcagctagg ccacggcctc
     1201 cccgttgcgc tcgttgtagt cgtcgcgcca ggcgacgagg tccttgaggg cggagatgtc
     1261 gtagtccggg ccgccggcgc ggaggatgag gtgcgtgatg ccgttctcgg cgtaggcgtc
     1321 gccgttcttg acctgctccg gcccgatgag gatggagcgc tctatctctt tcgggtcgcg
     1381 tccgaccttc tcgcaccagt cgtccaggat cctgctcttg cggcccgccg cttccgggtc
     1441 tccgaagccg ttccagatat gcgcgtgctc ggcgacgata cggagcgtaa ccttctcacc
     1501 cccgccgccg atgaggatcg ggatctcgcg cgtcggagcc ggattcagct tcccgaaccg
     1561 ctcctcgatg accggcagcg ccgcgtccag cttcttgagc ctccccggcg ccgtcccgaa
     1621 ctcgtaaccg tactcgtcgt agtccttctc gaaccagccg gagccgatcc cgagtatcag
     1681 ccgcccgccg gagatgtggt ccaccgtgcg cgccatgtcc gcgagcaggt tcgggttccg
     1741 gtacgagttg cacgtcacca gcgccccgaa ctcgactctc tccgtgacct ccgccatcgc
     1801 cccgaggagc gtccagcact cgaagtgttt gccctccggc tctccgtaga gcgggtagaa
     1861 atggtcccag ttgaagagcg tgtccgcgcc catctcctcc accctgagcc acgtgtcgcg
     1921 catctgcttg tactccgcgt gctgcggctg aatctgcgcc ccgaccctta ccgtcactct
     1981 ccgcctcctc acgtcgtcgc caaccccggc attatgcacc cctggaaggg atcaaaccac
     2041 agagaaccac ttgagggtgg ccgcccgctc gttggcgtcg accaccggac cgggtgtctt
     2101 cagacactcc tctacgcgcc ggtgcaactc cgtggggtct tcgccagcgc cggctttcag
     2161 cgggatcggg gacccctcga cggtgtacca ctcctccgcg cccgcgtacc tgacggacgc
     2221 ccgcagaccc tccccgactt ccgtggatag ctccaactcg cccgtaacgg caccggcctc
     2281 ctcggtcatg acgccgccgg tagttgactc gaaggtcgtc cgggtgccgc cgtccctatc
     2341 cgtcatccgc tagcctccgc agccgttcag catctggacg agcgactgtt tctcctgctc
     2401 gttcacgctc aggtcgtact tgtcctttat ctggacccag cgtttcgcgt agtcgcacca
     2461 ctcgcctttg ttgggcggtt tccacgcctc cggccccttg tcgcccttct cctggttggc
     2521 gttgtcctct accgagagga ggacgtccgg gtcgttggcg tagcgctcgc gctcggccgt
     2581 gtcccacccg gaggcgccgc ttctccaggc gttggcgagc ggaacgacgt ggtctatgtc
     2641 tatgtccagc ggttcggtgt aggtacgggt agtgtacggg tcgagccact tgccggagat
     2701 gacgtcgcag ccgcttccta cctggacgtc tttgccgtcc cggatcaggg cggcgtcccg
     2761 agcgtcgcag gaagggtccg cgtcccaccc ggactcctgc gcgtcgctcc agtgcgggaa
     2821 tttctcgcgc gagtacccgg ccatagagcc cggcggcgag accgttagac cgtcgagcgt
     2881 cttcaccacg ctggccgggg ccgctccagt atccaccttg ccgccgcccg atccgccgcc
     2941 ggtgttgtcc agctcggaga agacgccggt ccctcctaca gccaggatca gtaacaccac
     3001 gatcaaccgc acgatactct tactgctcaa cagctctccc gtcgtcttcg ccagccgacc
     3061 gagtttagca agacccgccg cacccggcga ccggaaaagc cgaggatcag gtatattgcg
     3121 ggccatgacc tcaccagaca ttgatgtcgg agacatccgc gccctcgcct tcgacgtctt
     3181 cggcaccgtc gtggactggc gctccaccat catccgggag ggcgaggagc ttggccggga
     3241 aaagagtctc ggcattgact gggccgcctt cgccgacgcc tggcgcggca ggtatgcgcc
     3301 ctcgatggac cgggtgcgcc gcggagagat gccgtggacg aacctcgacg ccctgcaccg
     3361 cgcctccctc gaagagctgc tcacggagtt cgggatagag agcctgacag aagcggagag
     3421 ggaccgcctc aaccgcgtct ggcataggct tgccccctgg ccggactccg tggagggact
     3481 gatgcggctg aaggaaagct atatcatcac cactctctcg aacggcaacg tggcgctcct
     3541 gacgaacatg gcgaagcggg cggggctgcc gtgggacctc gtactctccg ccgagatagt
     3601 tcgccactac aagccggacc cggagaccta cctcatggtc ccggagcttc tggacctccg
     3661 gccggatcag gtgatgctcg tggccgccca tcccgacgac ctgcgcgccg cccgagacaa
     3721 cggcctcggg accgcctatg ttctgcgtcc ccgggagtgg ggaccggatg gcgaggcaga
     3781 ggcccccgac ccgtccttcg acctggtcgc cgaagacttc ctggacctcg cgaggaagct
     3841 agaagcgctc aggtaacgag gttggcgaag acaaggcgca gcagccggac gtagcggggc
     3901 cgcgacgcca gtgcgaggcc gagcagggcg acggaagcca cgagcagcag gattcccgga
     3961 gcgataaacg gcgcctcgaa gcccacggac gcggccagga gaccaccgcc gagcggtccc
     4021 agcccgaagg cggcggagtt gacggacgcc gccacgccga aggcttcgcc ctgtctaccg
     4081 gacgccacgg cggagcgcac ggcgaggttg gcgaccggga tcaccagccc cagggcgaag
     4141 ctgatgatga tccgcagccc gaacagctcg gcgaccgagg taaccgccgc ctgcggcagc
     4201 gaagctagac cggcgaggag cagcgcgagg accatcacct tcatcgtgcc gaagcgggcc
     4261 gcgagccctc cgcccaccag cgaccctaca gccgcggcca gggctccggc gccgaggatc
     4321 tgtccggaga gacccgccac ctgcgcgccg gcccccgcca gctcccccac aaaacccggc
     4381 agcgccggag ccacgcccgt gatcgcgacg tttacgaaga agagcgggat cagaccgata
     4441 agcaacgccc gccaggggag cggtccggac tctctgacct cctcttcctc aggcgcggac
     4501 tccgcgccct cgaccccgaa gagcaccagc accccggaaa ggaagagcag cgccgccgtc
     4561 accccgaacg aggctcttat gccgacggcc tcggcgaaga taccgccgac gaacggcccc
     4621 gcggcggctg cgacgaatat gatcatctga agcgtaccga gccgcgcgcc agccttcccc
     4681 tgtggcgcgg tagccgacgc cagcactgtt gcggcggcga cagtaccggt gagcgtaccc
     4741 tgcacgagcc tgagaacgag caggtgccac gggctcgttg cgaggcccat caggccgacg
     4801 acgatggacc ccgcgaacgc cgcccgcata agcatcgtct tcgggccgag ccggtccgcg
     4861 agacggcccc acatgggagc cgcgagcgcc atcgtcaggg cggtggctgt attaagcacc
     4921 ccggcccagg cggcggcgga gccggcgtcc tccaccccga gccgctgcac gtagagcggc
     4981 atgaacggca tcacgaacat aaagcccgcc gtcgccgcga gttgagagat caggagtacg
     5041 gcgaaagcaa ggttgcccgc tacatgcacc tcggcccgct tcattggacg ggcctcagaa
     5101 cgaggatctc tacagtttcg gtctcaatgg aatcttgcat ctcataattt gagagtatca
     5161 aagttcgcgt ataggtccag cccggaaact ggccgggtgt gatcccttta acgcggcgct
     5221 gggacagcag ctatagcagc gcccagaccg cggtcgcgac gcccagggcg cccgtgagga
     5281 tgacgatgac gagggtcagt acccggaagg cgttctcgga gaagcgtttg aggaagctgg
     5341 tgccgagagc cttgccgagg aaggaggccg ggatcagggc gagcgccagc gggagttggc
     5401 ccgcgtccac cagcccccta agtgagagga ccgctatccc agcgatgctc gtgaagagga
     5461 agtagagcgc gctcgtcccc cggaagtcat gcttcgggag gccgcgcgag gcgaggagca
     5521 agactatcgg aggaccggcg aggcccgtcg aggtcgagag cgcgccgctg gccgttcccg
     5581 cgactagagt tccccaacgc gtatccgcgc cgggcaaccg cacctcccgg acgagcagta
     5641 gcgccgagat caccaccacg atgccgacac cgaggcggat gtatacggca tccacggccc
     5701 gcagcacctc cgcgccggcc acgatcccga ccagagcggg caggaacagc gagaccacga
     5761 gcctccggtg cgcctcccgc cacgagtccc agacgacggc gaggttaata aagatcgaga
     5821 gtatcgccga cagcacgatg accgtcctgg gctcgtagac gaagagcagg agcggcgtgc
     5881 tgatcagggc cagcccgaac cccgtgagcc cggagaccgt cccagccaaa aatgcggcga
     5941 ccagcgccac cagcagcgcc gcgctcaaca aggcggcgct ccagggacgc gaaagcctcc
     6001 gccggacggt ccccggtcgg aggttttcat ctcctgggag atctagcgca gtacgccggc
     6061 gtagaggcgt tcccaggcct ccatctccgg cgtgaagtag cgcacccgcg tcttcttgta
     6121 ctcgcgagtc gagtagaggg aggcgtagtc ggccaggccg ctctcctcgc tcatcgcgtc
     6181 caggacggcc tggcactcct ccacgctgcg gccgtggacc atgctgaaga gggcgtaggg
     6241 ccagtcctcg tagacggggc gctggtagca gtgcgagacg gcctggtact gggcgaacat
     6301 gttgccgacc tcttcgatgc gctcgtccgg gaccttccag acgcccatcg cgttcgcccg
     6361 gaagcccgcc ttgcggtggt agaggacggc ggagaagcgg cgcatgatct tgcgccgctg
     6421 caagtcgaac gcccgctgaa gaagctcgcc atagctcatc ccggcctgct ctccccagag
     6481 gtcgaaagga cgcggcgtga gcggcacgtc ctcctgcaaa gcccggatcg ccgccttgtc
     6541 ctcctcggag atgttcctgt cagcgccctg ccggtcggac tcgccgtact gcggggcctc
     6601 cttcttcgcc gtcgcgccct cgttcatgtc gagcgtcacc ccgatcttga agagcttgag
     6661 cgtgggcagg atgcgggtct tctcggcgcc gcttatgtgg tgcagcacgt caacggtgcc
     6721 ttcgagtccg agccgggagt ccggcgggac ggcgaccgtg taccagaggt tcagctcgtt
     6781 gttccgctcg tagttgtggg agacgccggg gtgggagttg atcgccttcg cgccggtagc
     6841 gagcttctcc ttcgggatct tggcggctac cagagtcgac tgataaccca ggacgcgcgt
     6901 gtcgaagatc gcggagatct gacgaatcac ccgccccctc ttgagcatct ctatccgccg
     6961 gatcacctcg tcgcccgcca gccctacctc tcgcccgatg gccgcgaacg gctcccgctc
     7021 cagcggaaac tcgcgctgga tgaagttcag gatgtccttg tcggtgctgt ccatcatgcg
     7081 actgccggta gttgtgctga ccaaggtata cgagcccttc tcgacggttt gctacttcat
     7141 aatcgcatca tagcaggcga tgccaaaata cggtatgaga atgtttacgc tttttcacga
     7201 gcaccgaacc ttcagccccg ctcctccatc gccttctggt actcggcgta gacggcctgc
     7261 acgtccttcc agaccaggcg cttggtcgcc ttgaggtcgt cgccgctctg gcgcaggacg
     7321 taggccgggt ggaaggtcgc catcgccttc gcgccgtgga cctcgtacca cttgccgcgg
     7381 tccttggtga tgcggaagtc cttatcgatc atggtctttg cggcgggacc gccgaggcag
     7441 aggataatct ccggttttac ggcgtctatc tgggcttcga ggtaggggtt gcaggccctg
     7501 atctcccccg tcttcggtgg ccggttcttc atcctcccgc cctcctggac cgccgcccgg
     7561 cacttgacga tgttggtgat gtatacgtcg tcgcgggtga ggtccaccgc tttcaggatg
     7621 tcgtcgagga gcttgcccgc ccgccccacg aacggcagtc ccgtggcgtc ctcgttctct
     7681 cccggtccct ctcctataag catcagaggc gagtgcgggt cgccggtccc gaagaccgtg
     7741 ttcgtgcggc tcatcgccag gtcgcacttc gtgcatacgg agacctggcg ggagagttcg
     7801 gcgagtctct gctccctgct ctcgccgttc tccgccgaat caaagagact gtccaatgct
     7861 cctcccgggc ttttccaggg tacggtttcc ctgtactatt ctaccgtcat acggcgagtt
     7921 ccggggaagg cgcgggcgtt tgagcatcat agagtttcac gcggtcaaca aatttttcgg
     7981 ggacttccac gtcctgaaag acatcgagtt ctcggtggac gaaggcgagg tcgtcgtcgt
     8041 catcgggccg tcggggtccg ggaagagcac gctcctgcgt tgcataaacg ccctggagga
     8101 gatcacgacc ggcgagctga tcgtggacgg catccgggtc catgataaga agaccgacct
     8161 gaacaagctc cgcacggaga tcgggatggt cttccagcag ttcaacctct acccgcacat
     8221 gacggtcgcc gagaacatca agctcgcccc cacgaaggtc aagggcgtct ccagtagcga
     8281 ggcgaacgac cgctgcaaga ggttactgga gcgggtcggc atccccgagc aggcgaacaa
     8341 gtacccgatc tcgctctccg gcggccagca gcagcgggtc gccatcgcgc gcggcctggc
     8401 gatggagccg aagataatgc tcttcgacga gccgacgagc gccctggacc cggagatgat
     8461 caacgaggta ctggaggcga tggccgacct cgccaaaggc ggcatgacga tggtcgtcgt
     8521 cacccacgag atgggcttcg cccggcgcgt cgcagaccgg gtggtcttca tggacgaggg
     8581 ccgcatcgtc gaagtcggcc ccccggagca cttcttccag aaccccgaga acgagcgcac
     8641 caagcgcttc ctcgcccaga tcctgcactg atagatcgga gcccgaactt gaagcccaga
     8701 tccgcgctgg atgccatccg cccctaccac ccgccgagga tggagtggga gatcgcggcc
     8761 gaccgaggcg cgaacgaata cgtgaagctg acgatgaacg agctctcctt tggtccgctc
     8821 cccgaggcga aggcggcggc actcgaagcc ctcgcccgcg gcaaccgcta ccccgaacgc
     8881 gactccgacc cgctgcgcca ggccatcgcc gaggcgaacc ccggcgtcgc gcccgacaac
     8941 gtgtttatcg ccaacggctc cagcgaggtg ctggtggacc tgatgcagac cttggagcgt
     9001 cccggcgagg tcatcttccc ctggccctcg ttccctctct actcttccgc ggcgcgcatc
     9061 gtcggcctcg cggaacgccg cgtacctctg gacgagcatc atgccgtaga cctcgacgct
     9121 ctgctggctg cggtcggccc cgagacccgc gccgttctcc tgtgcaaccc gaacaacccg
     9181 accgggacgc acctgccgct ctccgaggtg cgggcgttcg cgcgggcgct gcccgaggaa
     9241 gtgctcctga tcctggacga agcctactac gagttcgtca cggacccggc ctacccgggc
     9301 tcgcacgagc tggcgctgga atcggagaac gtcgtcacga cccggacgtt ctcgaaggtc
     9361 cacggcctgg ccggtctgag ggtcggctac gtcatagctc ctccgaaggt cgcggattac
     9421 gccggacgcg cgcacacgcc attttccgtt aacctgatcg cccaggccgc cgccgaggct
     9481 tcgatgcggg cgacggagag catcgccgag cgcgcccggt tcatggcggg agagcgggat
     9541 cgcgtccagg ccgccttcga tgcggcgggc ctggagtaca ttccctccca gacgaacttc
     9601 atcctggcgc gaatcggccc ggaggtcttc gagaaggccg gcgtcctggt gcgcgaggga
     9661 gaagcgctcg gatgtccggg atggagccgc atctcgctcg gcgactccgg ggagaacgac
     9721 cggctcctgt ccgcactctc ctgaacggga gctactcctc caccccgcac cagtcggcca
     9781 gcagcagcgc gacagtcttc gccgccgtga ggagatccgg gatactgata tgctcgtcgg
     9841 gagcgtgggc agcgttcacg tctcccgccc cgtacatcac gcacggcatc tctccgaacc
     9901 ggataaagag ccgcatatcc gcgccgtagg ggacgccctc tatggacggt ttgcgacccg
     9961 tcgtcagctc gtgggcccgt gcgaccgcct ggcagatcgg agcttcgggc ggtacttcgg
    10021 cgggtgcgaa ctggccgccg aaccactcta cctccggcgg gttctcgcgc agccaggggt
    10081 cccggcccgc gacggcgagg atgcgctcca cgacctgctg gcggaaaggt tcgacctcct
    10141 cgccggggat caggccgata cgtccctcgg ctaccagcgt ctcggggacg gtcgaggccc
    10201 agttgccggt tcgcactagc cccacgttga tcgggacctt gttcttcatc ccatcgtaga
    10261 gcggatgcga tagcgtcgcg ttgcgctcgc gctccagcgc ccgcaggtct tcgaagatgg
    10321 ggacgaactt ctccagcgcc gagacgcccg cgtcgcgcac agcggcgtgg gcggagcgtc
    10381 cgtggatggt gaggcggaag acgagcgagc cgccctgggc cgggacgagc gcgaggcgcg
    10441 tcggctccgt aatgagcgcg gcgtcggcgc ggtggccgcg caggacggtc gagagagccc
    10501 cgagcccacc gttctcctcc ccgacggtcg ccgccacctt cacgtcgccg cgcagctcta
    10561 tgccgaggtc cgccagcgcg tccagggcga ggaggtgcgt gacgagcccg cccttcatat
    10621 cgcacgcgcc gcgtccgtac aggaggttgc cgtccaactc gccggccgga tcgcgcgtcc
    10681 aggcggcggg gtctccgggg tccacggtat caatgtgggc gttcagcagg atcgatcgtc
    10741 ctccaccgga gcccgcccgt ctcccaacca cgttcgggcg gcccgcatac gtctcctgct
    10801 ccccgacatg ctccaggtaa ggttccatct cctcgcgggt cgcctcccag acgtccacat
    10861 ccaggccgcg ctcccggaag gcgtgc
//
LOCUS       DASSMB010000193         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1570790, whole genome shotgun sequence.
ACCESSION   DASSMB010000193 DASSMB010000000
VERSION     DASSMB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1570790"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1137
                     /locus_tag="VFJ72_11555"
     CDS             217..1137
                     /locus_tag="VFJ72_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HET7480140.1"
                     /translation="MSLIAKVLRRISWREGVKSGLRTTWELGKIIFPVTLVVSLLQYT
                     ALYDVLLSGLTPVMGLIGLPNEAAVPLALGNLLNLYAAIGAMLTMDLTVKQVFILALM
                     LSFSHSLPVESAVCRRIGVSVTLVFLFRIILAGSAAFAIGSLWSGGDEIARYGIAAAS
                     GEEPSGWIEVVLHALRAAGTGIVELAVIVFPVMMGIQILKNLGVLEKFAGLMRPLMGP
                     LGIAPRGAVTMAGGLVFGLAFGAGVIIQQVREQEFTRRELTLMILFLCACHAVIEDTL
                     IFVPLGIDVLPLLLIRLVTAVILTLLIARL"
     gene            complement(1178..1387)
                     /locus_tag="VFJ72_11560"
     CDS             complement(1178..1387)
                     /locus_tag="VFJ72_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480141.1"
                     /translation="MSEKDRDRVEYAVKQVGGRYYPVIIDREAGGHYEINNPMTGGVL
                     SYNNPQAAEKYIERARAKGRADGAG"
     gene            1580..1882
                     /locus_tag="VFJ72_11565"
     CDS             1580..1882
                     /locus_tag="VFJ72_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480142.1"
                     /translation="MITVTEGARELFLNVEHPEGTVLRLDPILDEATGETQVGISAGE
                     PQAEDQVVEHEGESLLHIAAPVSEALDGSTMDLVETPEGPSIGITPPGSAPVTDNS"
     gene            1955..2338
                     /locus_tag="VFJ72_11570"
     CDS             1955..2338
                     /locus_tag="VFJ72_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480143.1"
                     /translation="MDESATHVVIVGGPETTEELLAIVLDDSEAIPLFDSAEEAEEFL
                     TSTGDFGKDWRPKEVSARALIELLDYQDEEVEYVALSPPPERLTGGMEVNVIPRESLV
                     ELLKRQIGVEVQPESKGFLRRLFRR"
     gene            complement(2342..2824)
                     /locus_tag="VFJ72_11575"
     CDS             complement(2342..2824)
                     /locus_tag="VFJ72_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7480144.1"
                     /translation="MIVRALRETDHGPIIAVVNEWWYGRNVASKLPRLFFDHFQDTSF
                     VVEEDEEIVAFLVGFVSQSPARAAYVHFAGVDPGCRNEGLGRRLYETFFEKVRRRGCE
                     RVRCMTSPANRGSIAFHASMGFEIEEGDGGVDDVSVHTNYDGDGIDKVVFEDPILIGR
                     "
BASE COUNT          572 a          896 c          917 g          617 t
ORIGIN      
        1 cgccttcccg atcgcctcgg agaccttcat cgcccgtcct tttcatctcg tcgtgtcgaa
       61 gcgaacatta gggtagtgtg tagactcgcg caacagtcct caaagtcctc aagaatagaa
      121 gacggtcctg ccacgtgtat agtgagcccc tggcgcccga atcggaggag aagcattggt
      181 cacgttcgca ctatcctgta acagagagat accggcttga gcctgatcgc gaaggtgctg
      241 cgccgcatct cctggcgcga gggagtaaag agcggactcc gtactacgtg ggaactaggc
      301 aagatcatct tcccggttac gcttgttgta tcattgttac agtacactgc actgtatgat
      361 gtcctgctct ccggtctgac accggttatg ggtttgatcg gcctccccaa tgaagcggcg
      421 gtgcctctgg ccctcgggaa cctgctcaac ctctatgcgg cgataggggc aatgctgacg
      481 atggacctca cggtaaagca ggtcttcata ctcgctctaa tgttgagctt ctcgcactcg
      541 ctgccggtag agtctgcggt ttgccgcagg atcggggtga gcgtgacgct ggtgttcctg
      601 ttccgcatca tccttgccgg atcagccgcg ttcgccatcg gttcgctgtg gagcggtgga
      661 gacgagatag cccgttacgg gatcgcggca gcgtcggggg aggagccttc gggatggata
      721 gaggtagtac tccacgccct gcgcgcggcc gggaccggca tagtagagct agcggttatc
      781 gtctttccgg taatgatggg gatccagatc ctcaaaaatc tcggcgtgct ggagaagttc
      841 gccggtttga tgcggcccct gatgggacca ctcggtatag cgccgcgtgg cgcggtaacg
      901 atggcgggag ggctcgtctt tgggctggcg ttcggggcgg gcgtaatcat tcagcaggtc
      961 cgcgagcagg agttcactag gcgggagctg acgctcatga tcctgttcct ctgcgcctgc
     1021 cacgccgtaa tcgaggatac gctcatcttc gtccctctag ggatagacgt gttgccgctg
     1081 ctactgatcc gtctggttac cgccgttatt ctgacgctcc tgatcgcccg gctgtgacgt
     1141 cccagggcct ctggatagca ggctgagtga caacttacta tccagcgcca tcggcgcgtc
     1201 ccttcgctcg cgcgcgctct atgtacttct ccgcggcctg cgggttgttg tagctgagga
     1261 cgccgcccgt catagggtta ttgatctcgt agtgtcctcc cgcctcacgg tcgatgatta
     1321 cggggtagta ccggcctccg acctgcttga ccgcatactc tacgcggtcc ctgtccttct
     1381 cgctcatctc actctccggc tccgactatt cgtcattacc gtactacacg acgagcgtgg
     1441 tccatcgtca aatattccgg tatcccggtc ttgaatcgtg cgcgagactt gataggttga
     1501 ggggcgacac tgaacgctct gacccttcgt tgccgccgcg aaagacatgg cggcgagcgg
     1561 cgaggtggag gggagagaaa tgatcacggt aaccgaagga gccagagagc tctttctgaa
     1621 cgtcgagcac ccggagggca cggttctccg gctggacccg atcctggacg aggccaccgg
     1681 agagacgcag gtgggtatct ccgccgggga gccccaggca gaagaccagg tcgtcgagca
     1741 cgagggagag agcctgctgc acatcgccgc gcccgtaagc gaggcgctgg acgggagtac
     1801 gatggacctc gtcgagactc ccgaaggccc gagcatcggc atcactccgc ccggctccgc
     1861 gccagtgacc gacaactcat aagcgacacc cggctggcaa aatgggcctt gccttcccgg
     1921 cggggccctc cgtctgctaa cctgagcccc cgtaatggat gagtcagcca cccatgtcgt
     1981 aatagtaggc ggcccggaga ccaccgaaga actgctcgcg atagtgctgg acgacagcga
     2041 ggcgatccca ctcttcgact ccgccgaaga ggcggaggaa ttcctcacct caaccggaga
     2101 cttcggcaag gactggcgac cgaaagaggt atccgcgcgg gcactgatcg aactgctgga
     2161 ttaccaggat gaggaggtcg agtatgtcgc cctcagcccg ccgcccgagc ggctcacagg
     2221 cgggatggaa gtaaacgtga tccctcgcga atcgctcgtc gaactcctga agcggcagat
     2281 aggcgtggaa gttcagccgg agagcaaggg cttcctgagg cggttgttca ggcgttaagc
     2341 ttcaccgtcc tatcaagata ggatcttcga agacgacctt gtcgattccg tcaccgtcgt
     2401 agtttgtatg aacgctcacg tcgtccaccc cgccatcgcc ctcctcgatc tcgaatccca
     2461 tgctggcgtg aaacgcgatg gagcctctgt tggcggggga agtcatgcag cgcaccctct
     2521 cgcaaccgcg gcgccggacc ttctcgaaga acgtttcgta cagtcgccgc ccgagtccct
     2581 cgttcctgca ccctgggtcc acaccggcga agtgtacgta agccgctctt gcgggggatt
     2641 gggagacgaa cccgacgagg aaagcgacga tctcttcatc ctcctccacc acgaagctcg
     2701 tgtcctggaa gtggtcgaag aatagcctgg gcagcttgct ggctacattc ctgccgtacc
     2761 accactcgtt gacgacggcg ataattggtc cgtggtccgt ctcccggagc gctctaacta
     2821 tcaagcgttc tcctctcatc tacatacatt ttacgctcgg gaatcggcgt ttcggcgggc
     2881 ttcggagact gtccggttgt tcacgaacgg caggaaggcc agagcgacta cgatcatcac
     2941 ggcggcggag aaccagaagg gtgcggagag gccgaagtgt ttggcgagga ggccgccgat
     3001 ca
//
LOCUS       DASSMB010000194         5845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1443935, whole genome shotgun sequence.
ACCESSION   DASSMB010000194 DASSMB010000000
VERSION     DASSMB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1443935"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFJ72_11580"
     CDS             <1..291
                     /locus_tag="VFJ72_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET7480145.1"
                     /translation="QLGPEDISGGTFTVNNPGSLGSVVSTPIINYPQAAILSAEAIVK
                     RPVVIDDAIAIRGMMNLEVSFDHRILDGGVALKFLNAVKRRLEGYGPESSIG"
     gene            351..1448
                     /locus_tag="VFJ72_11585"
     CDS             351..1448
                     /locus_tag="VFJ72_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480146.1"
                     /translation="MQHDSLFGHLASRFSGQTENIATESLNYIINRSSVARRAFLQFV
                     AQANVELPDTLLFQTQAVGDDNAIPDLVGTDSESRQVLLVEAKFWAGLTGNQPVTYLK
                     RLPSQADGLLLFIAPALRLDTLWSELLRRCQDESVAIEQSPNDIAQDFREVRVGHTHT
                     LALTSWRAVLAYILRVLETEGEYRAVSDVQQLQGLCERMDSGAFLPLRSEELTSDTGA
                     RVRQYCEIVNAAISQAREADIASTEGLRPTSGPGFYGRYLRLQGHQCHLQFNADLWGR
                     LRATPLWLRIDHLQSKPWMKDALASLEREQPPRLIPGDRDLLVPINLPTGIEKQEVVS
                     AVFKQMKEVAELLRHYNQSEQETATGTSTSS"
     gene            complement(1541..2602)
                     /locus_tag="VFJ72_11590"
     CDS             complement(1541..2602)
                     /locus_tag="VFJ72_11590"
                     /inference="COORDINATES: protein motif:HMM:NF005757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7480147.1"
                     /translation="MIENPFYTHEYHGEYDLVSIGRLDLEEGGSIPDCQLAVTTWGEL
                     NEAGDNAILIPTWYSGTHQIFRDVYIGPDHALNPERYFIVCVNQIGSGLSTSPHNADG
                     PNAEIAMSAFPKVRIGDDVVAQERLLREHFGIEKLALVVGGSMGAQQTYEWAVRFPEK
                     VLRAAPIAGTAQNTPHDFLFTEVLNEAIWSDAGWNDGEYISNEDVVDGLIRHAHIWGV
                     MGFSTEFWKQEVWRALEFESMEAFLEGFLEPYFTVMDPNDLLCMAWKWQRGDVARHTG
                     GDLAAALGRITAKTFVMPIDEDMFFPPRDCKAEQALIPNSELRVVEDILGHLGLFGVA
                     PTFMPQIDQHLGELLATEV"
     gene            complement(2599..4266)
                     /locus_tag="VFJ72_11595"
     CDS             complement(2599..4266)
                     /locus_tag="VFJ72_11595"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7480148.1"
                     /translation="MSSVEQDRREAGVEERTTDFDAVVVGAGFSGLYMLHSLRNKLGF
                     SARVYEAGTDVGGTWYWNRYPGAKSDSDSYIYCYSFDEDLRQEWEWSERYPGHEEIRS
                     YLEHVADRFDLRRDIQFSTQVVEAVFDEDAKHWKIRTNKGDTVTARFFIAAVGSLSAS
                     NTPDFPGRDTFEGDTYHTAHWPHEGVDFTGKRVGIIGTGATAIQAIPLIAEQADHLTV
                     FQRTANYAIPARNGPVAPEVTAARKAEYDEIWDRLRQSYFGFEHYFIERSALDDSPEE
                     RERELRARYDDGGFGLWLGNYQDMFFVREANEAISEWVRERIQERVDDPETAEKLTPR
                     DHPFGTKRVPLESNYYEAFNRDNVELVDVREAPIEEITPTGLRTSDAEYEFDILVYAT
                     GFDAMTGPMNKINIRGRGGRLLRDKWAEGPRSYLGLTVADFPNMFTITGPGSPSVLSN
                     MPVSIEQHVEFISRILSDMRERGADVIEPTQQAEDEWTAHAQEVAHSTLLPQSATWYM
                     GANIPGKPRVFMPYLGGVGPYRQKCDEIAENDYEGFAFATQEERSGA"
     gene            4752..>5845
                     /locus_tag="VFJ72_11600"
     CDS             4752..>5845
                     /locus_tag="VFJ72_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480149.1"
                     /translation="MIPFMFLLYIVAFLDRVNVGFAALQMNKDLGFSDAVYGLGAGIF
                     FIGYFLFEIPSNLILERVGARVWIARIMLTWGVISAAMMFVTGPVSFYVLRFLLGLAE
                     AGFFPGMILYLTYWFPARERARRVALFMTAIPLAGVIGSPLSGLLLSLDGLAGLQGWQ
                     IMFLLEGIPAVILGVVVLFYMTDRPEHADWLEEGERGWLRDALERENRIKADHGEYST
                     RRSLTSGKVWLLAFVYFGVVTSVYGVGLWLPLIIKGFSGLSDLLVGLLAAIPYIAGVI
                     GMLLFARHSDATGERRWHVAIPTFIATVGLILTGLVGNSTLLQMLALTIAALGIYSAL
                     ATFWSLPTAFLSGTAAAAGIALVNSVGNLGG"
BASE COUNT         1027 a         1773 c         1826 g         1219 t
ORIGIN      
        1 cagctcggcc cggaggacat ctccggcggc accttcacgg tcaacaaccc aggctctctc
       61 ggaagcgtgg tctcgacgcc cataatcaat tacccgcagg cggccatact ctcggcggag
      121 gccatcgtga agcgcccggt cgtcatagac gacgccatcg ccatccgcgg catgatgaac
      181 ctggaggtct ccttcgacca ccgcatcctc gatgggggtg tcgcgctcaa gttcctgaac
      241 gccgtcaagc ggcggctgga aggctatggg ccggagagca gtattggctg attgcggctg
      301 aaagaagttt gtgcgcttcg taaacggtcg ttaaagaggg agagatagcc atgcaacacg
      361 atagcttgtt cggacatcta gcaagtaggt ttagcggcca aaccgaaaat atcgcaacag
      421 agtctctcaa ttacattatc aatcgctcct cggttgctag gcgcgcattc ctgcaattcg
      481 tcgcgcaggc taatgtcgaa ctgcctgaca cgttgttgtt tcagactcaa gcggttggag
      541 acgataatgc tattccagac ttggtcggga cggattctga gtcaaggcaa gttcttctag
      601 tcgaagccaa gttctgggcc ggacttacgg gtaatcagcc ggttacttat ctcaagcgtc
      661 tgccatcgca agctgacggt ttactgctct ttattgctcc agccttgcgc ctagacacgt
      721 tgtggtccga gctgctacgg cgctgtcagg atgagagtgt cgcaattgaa caaagtccca
      781 acgacatcgc acaagatttc agagaagtaa gagtcggaca tactcacacg ctcgcgctca
      841 cgagttggcg agcagtattg gcgtatatct tgcgagtgct ggaaaccgag ggagagtatc
      901 gcgctgtttc tgatgtgcag caactgcagg gtttgtgtga gcgcatggat agcggtgctt
      961 ttctgccgtt acgttcggaa gaactcacct ctgacacggg agcgcgcgtt cgtcagtact
     1021 gcgagattgt aaacgcagcg attagtcaag caagagaagc tgacattgct tcgactgagg
     1081 gactacgccc tacaagcgga cctggttttt acggacgcta tctacggctt cagggccatc
     1141 aatgccattt gcagttcaac gcggatctat gggggagatt gcgggcgacc ccgctgtggc
     1201 tgcgtatcga tcatttgcaa tccaaacctt ggatgaagga tgcgctcgca agccttgagc
     1261 gagagcagcc ccctcgactc attcctggcg acagagatct cttggtcccg ataaacctgc
     1321 caactgggat cgaaaaacaa gaagtggtat cagccgtatt caagcagatg aaggaagtag
     1381 ctgaactgtt gcgtcactac aatcaatcgg aacaagaaac agctaccggc acctctacga
     1441 gtagctagcc ttgtgttcga accacgcgta gtttcggacg ttgacaaagg tcaacgtttc
     1501 gcgccgggcg gtgtttgccg cccggtacga tggagatgcg tcagacctcg gtggcaagta
     1561 gctcgccgag gtgttgatcg atctggggca tgaaggtggg cgcgaccccg aagaggccga
     1621 gatgcccgag gatgtcctcg acgacgcgaa gctcactgtt cgggatgagc gcctgctcgg
     1681 ccttgcaatc gcggggaggg aagaacatgt cctcgtctat cggcatcacg aacgtcttcg
     1741 ccgtgatgcg tccgagtgcg gcggccagat ccccgccggt gtgccgggcg acgtcgccgc
     1801 gctgccactt ccacgccata cagaggaggt cgttcgggtc catcaccgtg aagtacggct
     1861 ccaggaagcc ttcgaggaag gcctccatag actcaaactc cagggcccgc cagacctcct
     1921 gcttccagaa ctcggtcgag aagcccatca cgccccagat gtgcgcgtgc cgaatgagcc
     1981 cgtcgaccac gtcctcgttc gagatgtatt ctccatcgtt ccagcccgcg tcggaccaga
     2041 tcgcttcatt gagaacctcg gtaaagagga agtcgtgcgg ggtgttctgc gcggtgcccg
     2101 cgatcggcgc ggcgcgcagc accttctccg ggaaccggac tgcccactcg taggtctgct
     2161 gcgctcccat cgagccaccc acgaccagcg cgagcttctc gatgccgaaa tgctcgcgca
     2221 gcagccgctc ctgcgcgacc acgtcatcac cgatacggac cttcgggaac gctgacatcg
     2281 cgatctcggc gttcggaccg tcggcgttgt gcggcgaggt cgagaggccg ctgccgatct
     2341 ggttgacgca cacgatgaag taacgctccg ggttcagcgc gtggtccggc ccgatataga
     2401 cgtcgcggaa gatctggtgc gtccccgaat accaggtagg gatcaggatc gcgttgtccc
     2461 cggcctcgtt gagctcgccc cacgtcgtca ccgcgagctg gcagtccggt atggaaccgc
     2521 cttcctccag gtcgaggcgg ccgatgctga ccaggtcgta ctcgccgtgg tactcgtgag
     2581 tgtagaacgg gttctcgatc acgcgccgga cctttcttct tgggtggcga aggcgaagcc
     2641 ctcatagtcg ttttcggcta tctcgtcgca cttctggcgg tacggcccga cgccgcccag
     2701 gtacggcatg aagacgcgcg gcttgccggg gatgttcgcc cccatgtacc aggtggcgga
     2761 ctgcgggagc agcgtcgagt gggccacctc ctgcgcgtga gccgtccact cgtcctcggc
     2821 ctgttgcgtt ggctcgatga catcggcgcc gcgctcgcgc atgtcggaga ggatccggga
     2881 gatgaactcg acgtgctgct ctatggagac cggcatattc gagagcaccg aggggctgcc
     2941 ggggccggtt atggtgaaca tgttggggaa gtccgcgacg gtcagcccga ggtaggagcg
     3001 tggaccctcg gcccacttgt cccgtagcag tcgcccgccc ctgccgcgga tgtttatctt
     3061 gttcatcggg ccggtcatcg cgtcgaagcc ggtggcgtaa acgaggatgt cgaactcgta
     3121 ctcggcgtca ctggtgcgaa ggccggtcgg tgtgatctcc tctatcggcg cttcccgcac
     3181 gtccacgagc tcgacgttgt cgcggttgaa ggcttcgtag tagttggact ccaggggcac
     3241 ccgcttcgtc ccgaacgggt ggtcgcgggg tgtcagtttc tcggcggtct ccgggtcgtc
     3301 gacgcgttcc tggatcctct cgcgcaccca ctcggagatg gcctcgttgg cttccctgac
     3361 gaagaacatg tcctggtagt tgcccagcca caggccgaag ccgccgtcgt cgtaccgggc
     3421 ccgtagctca cgctcgcgct cctcggggga atcgtcgagg gcgctgcgct ctatgaagta
     3481 atgctcgaag ccgaagtacg actggcggag ccggtcccag atctcgtcgt actccgcctt
     3541 gcgggccgcc gtcacctcgg gggccaccgg gccgttgcgg gccgggatcg cgtagttcgc
     3601 ggtgcgctgg aagacggtga ggtgatccgc ctgctcggcg atcaggggta tggcctggat
     3661 cgcggtggcc ccggtcccga tgatgccgac ccgcttgccc gtgaagtcca cgccctcgtg
     3721 cggccagtga gccgtgtggt aagtgtcgcc ctcgaaggtg tcgcggcccg gaaagtccgg
     3781 tgtgttcgag gccgacagcg acccgacggc ggctataaag aaccgcgcgg tgacggtatc
     3841 gcctttgttc gtccggatct tccagtgctt cgcgtcctcg tcaaagaccg cctcgaccac
     3901 ctgcgtggag aactggatgt cgcggcgcag gtcgaaccgg tcggccacgt gctctaaata
     3961 gctccggatc tcttcgtgcc ctggataccg ctcgctccac tcccattcct gccgcaggtc
     4021 ctcgtcgaat gagtagcagt agatgtaact gtcagagtcg ctcttcgctc ccggataccg
     4081 gttccagtac cacgtcccgc ctacatcggt cccagcctcg tatactcgcg ccgagaaccc
     4141 aagcttattc cgcaagctgt ggagcatgta caaccccgag aacccggccc caacgacaac
     4201 cgcgtcgaag tcggtggtcc gttcctctac gccggcttcc cggcggtcct gttccacgct
     4261 agacattctc tcctcctggt tgtggttcag agttcattct ctctatgccg gcacaccgcc
     4321 ggaaccagcc gtcttcgagc ctcgttaggc gggtctcttt tgagcgaatg cagtaatcac
     4381 tcgggcgtga ggcatgtgct cgagtgcccc gtgcattggt tgccggacct tcccctttcg
     4441 cgaagtacca ttgccatcac cccaagtaac gatggtaact taacctggaa cgagggtcaa
     4501 ctacgcgcgc atgacgtatc tctgttggta tgtgcgtcgg agacccgcaa gcccaaagag
     4561 aagatctcgg ttgtcaactc acctttcaga ccgtcgtcga gttggccggc ccggactttt
     4621 cggcggtcgg cgccttgaac cgggcgggat gtgccgctaa cctgactctt gtgggcaagg
     4681 ggagaaggag agtggttagc gtggatagcg tggcggttgg gcggaggacg ctttcgaagg
     4741 ttacgtggcg gttgataccg tttatgttcc tgctctacat cgtggcattc ctggaccggg
     4801 tgaacgtcgg gttcgcggcc ctgcagatga acaaggacct cgggttctcc gatgcggtgt
     4861 acgggctcgg ggcggggatc ttctttatcg ggtacttcct cttcgagata ccgtccaacc
     4921 tcatactgga gcgcgtcggg gcgcgggtct ggatcgcgcg tatcatgctt acctggggcg
     4981 tcatctctgc ggcgatgatg ttcgtgaccg gcccggtgag cttctacgtg ctgcgcttcc
     5041 tgctgggtct cgccgaggcc gggttcttcc ccggcatgat cctctacctg acctactggt
     5101 tccccgcccg cgaacgcgcc cgccgtgtcg ccctgttcat gacggccatc ccgctggcgg
     5161 gcgtgatcgg ctccccgctc tcggggttgt tgttgagcct ggatgggctt gcgggacttc
     5221 agggatggca gattatgttc ctcctcgaag gcataccggc cgtcattctg ggcgtcgtcg
     5281 tcctcttcta catgaccgac aggccggagc acgccgactg gctggaggaa ggcgagcgcg
     5341 gctggctgag ggacgccctg gagcgcgaga accgcatcaa ggccgaccac ggtgagtaca
     5401 gcacgcgccg gtccctcacc agcggcaagg tctggctgct cgccttcgtc tacttcggag
     5461 tcgtcaccag cgtctacggc gtgggccttt ggctgcccct gatcatcaag ggcttctcgg
     5521 ggctcagcga cctgctggtg ggtctgctcg ccgcgatacc gtatatcgcc ggcgtgatcg
     5581 ggatgctcct cttcgcccgg cactcggacg cgacgggcga gcggcgctgg cacgtcgcca
     5641 ttcctacttt catagccacg gtggggctca tcctgacggg actggtcggg aactcgaccc
     5701 tgctccagat gctggccctg accatcgcgg cgctcggcat ttacagcgcg ctggcgacgt
     5761 tctggtcgct gccgacggcg ttcctgagcg gcacggcggc ggccgcgggg atcgcgctgg
     5821 tcaactcggt cgggaacctc ggcgg
//
LOCUS       DASSMB010000195        60508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1189306, whole genome shotgun sequence.
ACCESSION   DASSMB010000195 DASSMB010000000
VERSION     DASSMB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 60508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60508
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189306"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..374
                     /locus_tag="VFJ72_11605"
     CDS             195..374
                     /locus_tag="VFJ72_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480150.1"
                     /translation="MKEALYGISLRKTEKYEDLARRAQESDDQELAEFFDRIGEEDHR
                     LAEEARRLLAQRVAE"
     gene            407..1108
                     /locus_tag="VFJ72_11610"
     CDS             407..1108
                     /locus_tag="VFJ72_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HET7480151.1"
                     /translation="MRIAFIGPPGAGKSTQAKRLARSLPHWNTSPRLSTGELVRAQIE
                     AGTDLGRELEERYERGERVPNETILSLVLPRVRRSGGWILDNFPADVAQAKALDEEIE
                     ERGAGGISRVISLEGPSEEELIRRVLGGRVTSRATGEVYHLQNDPPPEERERQDPGPF
                     EKRGDDTEESLKRNMVAYRREAAALKEYYEARGVLSVVDARRSMDEVAEEVLEALGHP
                     ERSEFYAALRKSKTA"
     gene            complement(1131..2228)
                     /gene="menC"
                     /locus_tag="VFJ72_11615"
     CDS             complement(1131..2228)
                     /gene="menC"
                     /locus_tag="VFJ72_11615"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET7480152.1"
                     /translation="MRIAGADLYRYSLPLTGPLMLKGATLRRREGLLLRLTGEDGAVG
                     WGETSPLPGFSRESLGAALGELRRLAAAMVGRDMEDAWTRADGTLARELDDTNPAPSV
                     RFGLEAAAWNLYAAASEVTLPELVSASPRDIVSVNGLLAGPRAEVLQEARRMQDAGYE
                     AIKLKVGGLSVEEDISLVREVRSVIGESVSLRLDANRAWDFEEVLRFARGVEGVGIEY
                     IEEPLADPDGLKEFASVCGIPVALDESLAGMAPEMLVEHGYAGAVVIKPTLLGGISQA
                     LRMASRAREIGARPVISSAYETGVGTAALVALTAGIGETPAGLDTYRRLAEDVVERRL
                     PLPAPRLDVRDAVTRGRGVVYWRISRVEPPR"
     gene            complement(2250..3101)
                     /gene="menB"
                     /locus_tag="VFJ72_11620"
     CDS             complement(2250..3101)
                     /gene="menB"
                     /locus_tag="VFJ72_11620"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET7480153.1"
                     /translation="MTNITWEPAGEYEDIKYEKAEGIAKITINRPEVRNAFRPLTVIE
                     MQRALEDAREDHEVGVIILTGEGPEAFCSGGDQKVRGDAGYLEDPTDPRRTPGGASVG
                     RFHVTDLHVEIRRCPKPVVAMVAGWAVGGGHVLHVICDLTIAADNAKFGQVGPKVGSF
                     DGGFGASVLTQLVGPKRAKEMWFLCRMYSAEEAYEMGLVNKVVPLAELEAETVSWCRE
                     MLDRSPFALRMLKASFHAHEDGFAGIQQLAHDANLLFYGSEEAQEGRDAYKEKRKPDF
                     SKFPRRP"
     gene            complement(3094..3951)
                     /gene="menH"
                     /locus_tag="VFJ72_11625"
     CDS             complement(3094..3951)
                     /gene="menH"
                     /locus_tag="VFJ72_11625"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HET7480154.1"
                     /translation="MEEDPALPSLRVSLPAGSRLRYESRGDPGRPPVLFLHGFMGASE
                     DWRAVMTALEDRFFSVSVDLPGHGGSVGLLPGSYAFESAARMVLDVLDELEIDRAAFV
                     GYSMGGRLALYLALRYPERYGGLLLESASPGLESEEARTERRIADERRAARLETGDFD
                     EFLRDWYLQPLFASLARDKELLRRTIEARRSNDPRELAKSLRGMGTGSQPSLWKELAG
                     LDVPMLAVAGELDGKFAGISHRMRESNPQVRTAIVPCAGHNVRLEACEAYTTLLKGFL
                     SDIYNPFHD"
     gene            complement(3915..4562)
                     /locus_tag="VFJ72_11630"
     CDS             complement(3915..4562)
                     /locus_tag="VFJ72_11630"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET7480155.1"
                     /translation="MIKINSIFTGEPRTITDERGTWRSAIFRAPVDGEVRVTVRGLEG
                     DRVADTKNHGTPDQALCCHPLGHYAFWNEFYGLSGEDAVGPGGVGENWTLFGDDESGI
                     CVGDVYAAGTSRVQVSGPRVPCWKQERKLGLPGFHRRTLESLRTGFYMRVLEEGTVRT
                     GDELRIEDRPNPGITQRLVNECAHGSFDPALARRLLEVPELADGWKKILRSRLSG"
     gene            complement(4602..6353)
                     /gene="menD"
                     /locus_tag="VFJ72_11635"
     CDS             complement(4602..6353)
                     /gene="menD"
                     /locus_tag="VFJ72_11635"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HET7480156.1"
                     /translation="MTPKSTPHLNRLWAALIVEELARCGVGLFCVAPGSRSTPLVAAL
                     AENDRARSLIHFDERGTAFAALGYARATGSPAAWITTSGTAVANGLPAVVEASVDGAP
                     MILLTADRPPELRQTGANQTIDQPDIFGDYVRWRFDLPAPDPAIDPAMVLTTVDQAVY
                     RSRRSPSGPVHLNLMFREPFLPDPGDEAEAPPGPESWRESNVPYTRYAATKPAVDETE
                     VRRIREELRTVERGVVIAGRLTSRAQGESVIKLAEALGWPLLPDVGSQARLGAESRNV
                     VAHYDALLASETFAEAHAPEAVLHIGGRALSKRLEKLVARSRPQPYIVVRENPFRLDP
                     GHRVTQSVEMDIVDFCAAFDGVSPLQHGWLAGWREASGKVSRSLDKLLPETEDLSEPL
                     VARLVSRHVPKDHGLVAASSMPVRDLDTFAVTDGAGVLVAANRGASGIDGTVATAAGF
                     ARGRGAPVTLIIGDLALLHDLNSLAMLRDALVVVIVVNNDGGGIFSFLPVAGHRGFFE
                     PYFGTPQGVGFGPAATMFGLGYESPRTGTEFVKAYRAACARGSSTLIEVKTDREENVA
                     LHRELVEEIHRDLNGGV"
     gene            complement(6325..7734)
                     /locus_tag="VFJ72_11640"
     CDS             complement(6325..7734)
                     /locus_tag="VFJ72_11640"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HET7480157.1"
                     /translation="MLSRYIRDSVFERTDVRWTLADAVSRALALNTGLQRGERVLRLE
                     VPAGDLDPLRWLGAQTMRPRSFWSGREDEISVAAVGVADVVEGERPADVDELTDRLAL
                     LSPEAANARYYGGLSFDAGSEVSEEWQVFGARRFVLPRFELRRRDGVSTLACNLVLPR
                     DLEKAGDLLYEIENLPLKGPRLAGSLPAPVSRADSPDREGWRENIESALAAFAAGRMD
                     KVVLARRAEFAFEEDVDAVALVASLREATPGCFHFLVEPEGGAAFVGASPERLFRREG
                     RGITSEAVAGTRPRGASVADDDELRDELLRSVKDQAEHSYVRISIEETLGPLCAELEI
                     EDHPSEMKLARGRHLVSKIRGTLREGVTDAEVLAKLHPTPAVGGYPKNVALEEIRKLE
                     PFDRGWYAGAIGWIGADGAEFAVGIRSGLVRGSRVSLYSGAGAVAGSTPEGEWAEIEQ
                     KIGDFTRVFGLDPEEHAAP"
     gene            complement(7823..9283)
                     /gene="xylB"
                     /locus_tag="VFJ72_11645"
     CDS             complement(7823..9283)
                     /gene="xylB"
                     /locus_tag="VFJ72_11645"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET7480158.1"
                     /translation="MLLGLDLGTGSVKALLLSSEREVLGEGSAPYDVLAPRSGWAETD
                     PEEWWNGVIRATRTAIGERGGEVRAIGLSGQMHGVVLSEAGGRPLRQAILWADTRSAE
                     QLTVYQALGDDLRRRLANPAATGMAGPSLLWLRDHEPDVYARARWALQPKDWLRLRLT
                     GEAAAEPSDASATLLYDLEGDGWAYEVVEALGLRDELLAPLVPSGSVAGPLSREAAEA
                     LGIGAEIPVAAGAADTAAAMLGSGLMRSGVAQLTVGTGGQIVVPRERPEPDPHERTHL
                     YRAATDTGWYAMAAIQNAGLALEWARRVLGAGWDEVYEEAFAVPPGAGGVAFLPYLSG
                     ERTPRFDPSARGTWTGLGLDHTRGHLLRAALEGVAFALREGLEALEETGAGIPELRLA
                     GGGTARKPWRQLLADVLGRPLRLLPENVASVASARGAAILAGLASGALRVEDIVTPGD
                     TTGEVVRPGEESGEYEAAYARYREIYPRLHEPRPGM"
     gene            complement(9472..10077)
                     /locus_tag="VFJ72_11650"
     CDS             complement(9472..10077)
                     /locus_tag="VFJ72_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET7480159.1"
                     /translation="MLTVLVMVAGLAACGSFGQADSQADKKMSEQAKDQQVAPKKGDE
                     KSGKKVEDIARVPSDPTLKLTIPKMERIKDRKIPVGEGTDEALLRENAAIHLKGTGNP
                     WEKEANVYIAGHRLGYSGTGSYLAFYDIDKLKDGDQIYLTDADGQKYTYKVFKTMVVK
                     PTDLYVLNPVKGKNIVSLQACTLPDYSNRVIVQAELEDVKA"
     gene            complement(10207..10926)
                     /locus_tag="VFJ72_11655"
     CDS             complement(10207..10926)
                     /locus_tag="VFJ72_11655"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET7480160.1"
                     /translation="MVLRKMVLLVAALTMVVGLAACGSSGQSGGDQGSKASDKKSEKA
                     APKKESDTKKSDAKKSDAKKDSGKGEKKLVAKVPKEKTLGLSIPKLGKDFKDLPTGQG
                     DDTQMLTNNAAIHIRYPTKSGGFATGWPWKKEANVYFAGHVEGYQGTPSYKAFDGIGT
                     LEKGDDIIVTDADGQKYTYKVSEKKIVHPTEVDVLNPVPGKNVITLQTCEIVNVDANG
                     NPDYSNTERLIVRGELKDVEA"
     gene            11356..11859
                     /locus_tag="VFJ72_11660"
     CDS             11356..11859
                     /locus_tag="VFJ72_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480161.1"
                     /translation="MATMEKTNKAAERLAKATSDSYKTVIDHTVALQERNVRFAQGLV
                     DGAAGELRHQAESNRSLVEELVERAEKQRGAFQTLVEESVDAYMDFVYAPFSYYKQGL
                     RLIETEVTQGGFPIPNYDDLNVGEVTKLIDSLTAEQIRTVREYEKKNKNRETLIEQFD
                     RKLRPAS"
     gene            complement(11958..12146)
                     /locus_tag="VFJ72_11665"
     CDS             complement(11958..12146)
                     /locus_tag="VFJ72_11665"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7480162.1"
                     /translation="MSRRRNTANQDKGEGKMDKAAGRLKEAAGALTGDRTKKAEGRAD
                     QRKGTMKEKKGHLKDLLK"
     gene            12412..12618
                     /locus_tag="VFJ72_11670"
     CDS             12412..12618
                     /locus_tag="VFJ72_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480163.1"
                     /translation="MNAMISEHKETLAKMLVALFLAASLLIAAVGPADAAARSKCRNN
                     PENFSFFHGKCLSDKRIEKLTGGE"
     gene            12793..13341
                     /locus_tag="VFJ72_11675"
     CDS             12793..13341
                     /locus_tag="VFJ72_11675"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET7480164.1"
                     /translation="MNVAAMTRVALMVAVTAAAAQIAIPLPFSPVPFTLQVLAVILSG
                     LLLGPRYGALSQALYVLVGSIGVPVFAQFHGGLGVVLGPTGGYLLSYPFAAAIAGLAA
                     HAAANSPRRRALTRSFVWGTIGLAVIYALGAAWLSVVADLPVAAAVAQGVLPFVVFDV
                     IKVGLAALVAVAAAPAIATSRV"
     gene            complement(13414..14754)
                     /gene="gabT"
                     /locus_tag="VFJ72_11680"
     CDS             complement(13414..14754)
                     /gene="gabT"
                     /locus_tag="VFJ72_11680"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HET7480165.1"
                     /translation="MLESTRIKTEIPGPKSRALMERRHRAVTSGIGIATPMFVQEARG
                     ALLTDVDGNTFIDFGGGIGVLNVGHTNARVVEAVKEQADRFLHTCFYVTEYEPYIELA
                     EKLNALVPGDFKKRSMFINSGAEAVENAVKIARAYTGRPAVIAFENAFHGRTMLAMSL
                     TSKTDPYKKSFGPFVPEIYRVPAPYPYRCPAGKDCSGGCQGDCFNALEKAFVSYVHPS
                     SVAAIIGEPVSGEGGFIPFPDFYLRQLRELCDEHGIVLIVDEVQTGFGRTGKMFAIEH
                     SGVIPDIVTTAKSLGGGMPIGGVTGKAEIMDAVHTGGLGTTYGGNPLACAAALAVIEE
                     FEESDILGKANALGERVMGAMRDIQQKHPDFVGEVRGRGAMAAMELVKDAGTREPDKE
                     RTGKVVETALQEGLLLLTAGQYGNVIRTLMPLNIADDELEEGLKILARTFDSVA"
     gene            complement(14930..15064)
                     /locus_tag="VFJ72_11685"
     CDS             complement(14930..15064)
                     /locus_tag="VFJ72_11685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480166.1"
                     /translation="MIETLFVGGEWTERIASGKTFDVTNPANGEVLTTLTDAGRDEGR
                     "
     gene            complement(15061..16011)
                     /gene="speB"
                     /locus_tag="VFJ72_11690"
     CDS             complement(15061..16011)
                     /gene="speB"
                     /locus_tag="VFJ72_11690"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HET7480167.1"
                     /translation="MTSYEPADSFETPRFSGVRTFMRLPNTQDLENADVAIVGAPFDT
                     GATFRAGARFGPEGIRAVSHLLRRYNPSLGVGIFDHLSVIDYGDVPVVPGYIEASYQK
                     IAEGLETIHRAGVIPVVLGGDHSIALPELRAAAAVHGPLALVQFDSHPDTWDAYFGQK
                     HTHGTPFRRAVEEGLLRPERSIQVGMRGSIYDERDWDDARQMGFELVPTDAVRKIGIP
                     ETIERIRERVDDARIYVSFDVDFVDPAFAPGTGTPEIGGFTSREAQEFARGLAGLNIV
                     GCDVVEVYPQYDPAQITALLAANIAHEFLSLIAHSRVEKQ"
     gene            complement(16164..16643)
                     /locus_tag="VFJ72_11695"
     CDS             complement(16164..16643)
                     /locus_tag="VFJ72_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7480168.1"
                     /translation="MDTMFPTKILIASDDSDTAALAARVAGEIAEKTGSELHVVFVEP
                     VVAFAAGQRIYEELYDEVKKAAQEILDEEVKRIEETGATVAQAHLRLGNADEEIIALS
                     DELDSHLIIMGVRGSGPLRRLLGSTSDSVLRHANCPVMVVRSPRESRGRHGSRQGGG"
     gene            complement(16863..17984)
                     /gene="ald"
                     /locus_tag="VFJ72_11700"
     CDS             complement(16863..17984)
                     /gene="ald"
                     /locus_tag="VFJ72_11700"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7480169.1"
                     /translation="MARVVGVPKEIKDKEGRVSMQPDGVAELVHHGHEVVVQSGAGAG
                     AGFTNGEYADAGARMVQSAEEVFEAADLIVKVKEPIPEEYDRFKEGQQLFTYLHLAAD
                     KGLTEFLMEKKVNSIAYETVETPDGSLPLLTPMSEVAGRMATQAAAHCLESPQGGAGL
                     LLGGVPGTPAAKVTIIGGGVVGTEAAKIAVGMRAIVRVLDVNPKRLAYLSDIFEGRVD
                     LVIPNRARTASYVAESDVVIGAVLVHGAKAPKLVTRDMVASMRDGSVVVDVAIDQGGC
                     VETARPTTHSDPTYIEEGVVHYCVANIPGAVARTSTLALTSVTLPYLIRIADQGIEGA
                     AAADPALAKGLSTLDGNLVHEPTAEAHGLSYMDPAKLLR"
     gene            complement(18106..18735)
                     /locus_tag="VFJ72_11705"
     CDS             complement(18106..18735)
                     /locus_tag="VFJ72_11705"
                     /inference="COORDINATES: protein motif:HMM:NF015423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HET7480170.1"
                     /translation="MSPASVLHALDFFGVAVFAVSGALSAGRKSMDVFGVIVVALVTA
                     IGGGTIRDVLLDRHPVFWIEDPNYLVVILLAAAATIVYTRFQRPPRLSLLVADAFGLA
                     LFTVAGAQIAERAGVPSLIVVIMGAITGAAGGLLRDVLCAEIPMVLRRDIYATASLAG
                     AGAYVLLTALGVSAAISGPAAAALIFILRLISIKWDFNAPAYSLDDESG"
     gene            18897..19712
                     /locus_tag="VFJ72_11710"
     CDS             18897..19712
                     /locus_tag="VFJ72_11710"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7480171.1"
                     /translation="MATNGGTVGFSIGEVSQAIGLAPQTLRLWERERLIHPQRTGRGY
                     RVYSETDVERLRKIKHLRKTEGLNFAAIRKQIGTAETRTAPDTKRGARNSATPGEQLR
                     KLRIRAHKTLKEVSEATGLSISFISALERGGSGASVASLRLLAGAYGVSMREVFGADL
                     EQNSPLVRSDERPVMQWDNGVRFEEMASGEKVMDPSYIKVPPGGGSEGFYSHNGEEFI
                     YIISGTLFVELKDQGSYHLTPGDTLYFPSTTPHRWWAEDEPVEAIYVNTPPTF"
     gene            19723..21561
                     /locus_tag="VFJ72_11715"
     CDS             19723..21561
                     /locus_tag="VFJ72_11715"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7480172.1"
                     /translation="MSIDPHLDALLSMPVLSGPVVSPDGRWVAWNWSRLGPATDVFAA
                     PTDGSAPPLRMTGSHDDTQVVSWTPDSRAVLAAQDHDGDERARLFRVALEHPGVMEPL
                     TEESPGYYVRGGQTHPNGRWLIYGANFDVETGKEIEPTWVLRHDLKTGVRHTLARPER
                     SAFAEPQLSNSGEHVLYHRSDIHPEGRQVWLVDIEGHADREILNFGSRTKVSASWFPD
                     GRRILFLVEAETHRRIGVWDMDGESTRWLLDDPQRGIEYAFVPPNGGPVVAVEMREAR
                     VRSSLLNAESGEEARMPDLPGNLIPLAPANDGAWISLHYNSRNPVDLVRYSLKPGENP
                     SSLTRLWERTSLDPKSLAPAEDFRWRSVDGLEIQGWLYRATGRSIGTVVLVHGGPTSI
                     SEDRFNAQVQYLVGRGFDVLSPNYRGSIGFGLEFQEKIKEDGWGGREQEDIRTGIEAL
                     ISAGIAEPYRVGVTGTSYGGYSAWWAITHYPPETTAAAAPICGMTDLTVDYHTTRPDL
                     RPYSEEMMGGTPEDIPERYTERSPINFIQNVRGSLLIVQGLKDPNVTPENVQVVVEEL
                     DRRRIPYELLTFEDEGHGISRPDNLKVLYPRLADFFEEAFLEDRRR"
     gene            complement(21667..22362)
                     /locus_tag="VFJ72_11720"
     CDS             complement(21667..22362)
                     /locus_tag="VFJ72_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480173.1"
                     /translation="MAGGNLVRLGGICGLLSVILMIPAYIVGYPDAPGSLSEAASYFD
                     EGIGAFVLWNGVFPLFHVFFFVWFLGILHGMLRRAEGEAGGLSSAALAGGVMFATLAS
                     AGFAAEVLYPATLLRFEGFGQDTRLMLASLTLSAWLYHLCQVGTAVLVSATSLLALET
                     GVLPRWLALAGFVVALLALLHFLVPLLGALAGLVWIAAVSALMVAGSGGRSERSGRSG
                     RSERGPRRLSRDT"
     gene            22619..23983
                     /locus_tag="VFJ72_11725"
     CDS             22619..23983
                     /locus_tag="VFJ72_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7480174.1"
                     /translation="MDVMRGLGEHGESGSLAGVDNLLLHFTPFAEDWSKLPVIVSGDG
                     CYITDDRGNRYVDGLAGLFTTQVGHGRTELAEAAYNQMKELGFFPNWSFQHPRSLELA
                     DKLARIAPGDLSSTFFVSSGSEAVETVIKLSRQYHHANGEPTRYKVISRKIAYHGTTM
                     GALSVTGLPSFKAPFEPLLQGFHHVPNTQQDPEGAADAIEEAIEFASPETVAAVILEP
                     VQNAGGCLVPPPDYWKRVREICDRHGVLLVSDAVICAFGRLGEWFGIERFDVVPDMTS
                     FAKGVTSGYSPMGGVMVSERIQNALRSHEPMFMHGSTFGGHPVSSAVALENIKIIERE
                     KLLENVRNLEGHFGDELRRMAEGHPIVQEVRGMGFFWAVELKPERADGTPLEGEEYQK
                     YFKGVLSRRLVENGLICRFDDKEDPVIQYSPALIADREVLSKIAEITDDALSELEREL
                     GYRG"
     gene            24156..25328
                     /locus_tag="VFJ72_11730"
                     /pseudo
     CDS             24156..25328
                     /locus_tag="VFJ72_11730"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            25451..26593
                     /locus_tag="VFJ72_11735"
     CDS             25451..26593
                     /locus_tag="VFJ72_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7480175.1"
                     /translation="MEDRKTTHRQTPPAGSPEAGRGVAVRLAGVTKNFGEFVAVDDLT
                     LDIYEGEFFSLLGPSGCGKTTTLRMIAGFEEPSRGEISVAGEQVQGVPPYRRPVNTVF
                     QSYAIFPHLNVFDNVAFGLRRAGVKGDELRNRVMEACEMVQLQGFEKRQPNMLSGGQQ
                     QRVALARALVNRPKVLLLDEPLGALDLKLRKELQLELRTLQEEVGITFVYVTHDQEEA
                     LTMSDRIAVMDGGKVQQVANPATLYELPRNRFVAGFIGQTNVLSGAVETVNGERVTIR
                     AGGASGAEIEAVAHEEARVEPGGEAHVAVRPEKLRFGDSGDNVLSAEVVQIVYLGAST
                     QYIMKLANGGRLVLYQQNSHDAAVGAVVGDEVLVSCDAANCLVLGG"
     gene            26596..27711
                     /locus_tag="VFJ72_11740"
     CDS             26596..27711
                     /locus_tag="VFJ72_11740"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7480176.1"
                     /translation="MERNTSITRGRFLKVMGAGAVAGTTLSVLACQPNTNAQSSGGGG
                     DGGGGKPKKINLYNWSDYVAKSTLPGFEKKTGIKVQQDFYGSNEELLAKLQAGGTGYD
                     VIVPSDYMVSIMRKSEVIEKLDKSKIPNFKNLDPDFKGLPYDPNNDYSMTYQWGTTGI
                     LYDPEVVEEEVTGWDAMWDEKYSGKIGMLNDVRETFGASLKRLGYSLNSTDKGELDEA
                     QSELEKQKPLLRGYFGSPEGGDLVVGGDLALAHVFSGDGFSAIAENDKLVYIIPDQGA
                     TRWTDNMAIPKGAPNPDVGHQFINYILDPKVGADLSNYTYYGCPNKASMPMIDKAAKN
                     DPAIYPPPKVFERLEVIKDLGSATKDYERRFTEVKSG"
     gene            27713..28576
                     /locus_tag="VFJ72_11745"
     CDS             27713..28576
                     /locus_tag="VFJ72_11745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480177.1"
                     /translation="MRGRKGGWSLGLAGLLAPVTLWLGLFFLVPLLLILAYSFGTSGP
                     YGGITLGFNPGNYLNVFDPLYLQIVGRTFVIAAINTLICLLLGYPLAYFIAFKGRRRR
                     TMLILMVMVPFWTSFLIRAYAWVVILNGNGIANKTLQFLGITDGPINLIFTPGAVTLG
                     MAYAYLPFMILPLYAALEKFDVRLKEAAQDLGASRWHTFWRVTFPLSMPGVIAGSILV
                     FIPSAGEFVIPDLLGGSRTVLMGNLVQQQFLQARDWAFGSALAMILAALLLVAIMFYV
                     RKVGTEKLEGV"
     gene            28578..29417
                     /locus_tag="VFJ72_11750"
     CDS             28578..29417
                     /locus_tag="VFJ72_11750"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480178.1"
                     /translation="MAAVRGGPVYKGMGKLPDRALLVLSVFIYLFLYAPIAIVIFFSF
                     NDAKSTQNWAGFSTRWYGELIRDQTVLDAFYNSMIVGVTATAIATVIGTLTALALSRN
                     DFRGKTFADSAIYAATVMPEIVVGVSLLVFFVAAQIGLGITTIIIAHVAFTISFVTIV
                     VRARLSGMDRSIEEAAQDLGASPVQTFLRVTLPLILPGVMAGALLAFTLSFDDFVITF
                     FVSGVGSSTLPLKIYSMLKFGVTPVINALSTIVVFTTLVIILGAGRFFAGGDDLKNAR
                     KDS"
     gene            29521..30222
                     /locus_tag="VFJ72_11755"
     CDS             29521..30222
                     /locus_tag="VFJ72_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7480179.1"
                     /translation="MTNAPERIAPGVYRVDAVGLSNVINVLLLEDSGGWTLVDTGVGS
                     SAARIQDALSALGSGPEDLKSIFLTHQHNDHVGGLHAMLEWAPNAKVGASEHEADVIS
                     GRRGFDPQSSPVLRYLSRNAKPPGVTVQKVVRDGDLVSGFRVIPTPGHTRGHVSLLRD
                     EDGLLFTADAFGCLPRKLRVGVRKAFCTDPEQARRSAGKLLDEDFATVVFAHGKPLRT
                     GAKARLREVVAKCRY"
     gene            30242..31840
                     /locus_tag="VFJ72_11760"
     CDS             30242..31840
                     /locus_tag="VFJ72_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7480180.1"
                     /translation="MNAPTAAGGRYDAIVIGSGHNGIIAAAYLAKAGKSVLVLERRGV
                     IGGATVTEEPWPGYRLSTCSYVCSLLLPEVIRDLDLVRHGYDVRAFDPQYFVPFPDGR
                     YFMSHLDSAKTEAEIRKFSTKDVAAYGAYWRMWDRIIARMRPLLMKSAPTQADIERAF
                     DGPEGEEDWRTLTKKSIAEVLDEYFESEEVKAPLCVGGVIGTNAGPRTPGTAYVKYHH
                     IIGNVGGNQGAWGFVRGGMGSVAHSIASSAREQGAEVLTDAEVESIEVEDDSARGVTL
                     RDGRRFEAGVILSNADPHRTYLGMVGERHLPQDLAEGVKRINVKGSVVKVLLALGELP
                     DFTALPGKNVGPQHTGGIVINPSVDYLQRAWEDCEGGRPSERPFMEGYVQSATESGLA
                     PEGRHTMSLFCQYTPYTLDKGTWEERREEIGANIVETFAEYAPNLPDAIEHMEVLGPP
                     DIEARIGITGGNIFHGEILPEKMFAGRPVPGYSDYRTPINNLYLCGSGAWPGGAVFGA
                     PGRNCALEALADLDVKTARARHVS"
     gene            31864..33297
                     /locus_tag="VFJ72_11765"
     CDS             31864..33297
                     /locus_tag="VFJ72_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HET7480181.1"
                     /translation="MDTKSKLQNFIGGDRVDPANGRLYELVNPSTGEVFAEAPASSKE
                     DVDRAFEAAEKSFETWRDSTPSQRQLALLRLADAIEERAEDLVRAESENTGKPIGLTM
                     EEEIPAMVDQIRFFAGAARCLEGKSAGEYMSGMTSFIRREPVGVCAQVTPWNYPMMMA
                     VWKFAPAIAAGNTVVLKPSDTTPVTTVMLAEMAAEFLPEGVFNVVCGDRDTGRALVEH
                     PTPQMVSITGSVRAGMEVAKSAAADLKRVHLELGGKAPVIVFDDADIEAAAEEISVAG
                     YFNAGQDCTAATRVLAAPGIHDDFVAALTEQARGTKTGAPDDEDVLYGPLNNPNQLQH
                     VSGMVERLPDHAEVVTGGQKVNGSGFFYEPTVVSGLRQDDEASQNEIFGPVITVQAFS
                     DEDEAVRWANGVKYGLASSVFTRDHGRAMRVSRRLDFGCVWINTHIPLVAEMPHGGFK
                     HSGHGKDLSMYGLEDYTRIKHVMTNLG"
     gene            complement(33479..33823)
                     /locus_tag="VFJ72_11770"
     CDS             complement(33479..33823)
                     /locus_tag="VFJ72_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480182.1"
                     /translation="MAGRWCVLNQPIGRRRRDLRPGLLAPGRRSKPQKLSLCSSYALK
                     ILLFALLFRLFPRDMTFFSQQRKESVLSFAQVLQALLGQIHIPARGPKGESGDYKSCG
                     ADHGKRTDTEEK"
     gene            33947..34330
                     /locus_tag="VFJ72_11775"
     CDS             33947..34330
                     /locus_tag="VFJ72_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480183.1"
                     /translation="MWRPHPDDDIALGRAAERTRSQSDSPVEMFRRHYDKYLENYEGY
                     VKLAERIHRMRRESGVAPDSPAGREWEERLRRANDGIDRTIARLDILEEYNPELATDD
                     RISRRASIAHSHWKLDRGKPEHRDS"
     gene            complement(34378..35031)
                     /locus_tag="VFJ72_11780"
     CDS             complement(34378..35031)
                     /locus_tag="VFJ72_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480184.1"
                     /translation="MELTRVLIADDHPHFRDGLRALLLSAPDVEVVGEARDGEEAVKL
                     AAKLQPDVILMDLNMPGVGGIEATRRILRTSPHISVLVISMYEDDDSVFAALKAGARG
                     YLLKGALKAEILRAIRSVMSGEAIFGAAIARRLMQYFASPGPAAAKDAFPELTEREHE
                     ILELIACHETNPEIAKKLHLSQKTVRNHVSNIFTKLQVADRAQAIIRAREAGLGKEM"
     gene            complement(35034..37229)
                     /locus_tag="VFJ72_11785"
     CDS             complement(35034..37229)
                     /locus_tag="VFJ72_11785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET7480185.1"
                     /translation="MSRAGGPPPVLAATLAEEVMGSEAATPDTPRAPIAARVGGRVLA
                     GISLVLFVLSIPARYMELVETARRASTQLGPGLGSLQSFLSEGTYYAIAVLFLEILFV
                     LSLTLISIAIAWRNKPDWPSLFFSTVFVAYAVWVTQTLDALVLPSVLQVIANLTQAAG
                     VLLAVTFFLIFPDGRFVPGWTRASAVAWFFYCLAWGIFPTAWFSLIDPFEASFAAFLI
                     LLLLGWGLGLMAQAVRYRRADQRQRIMTKWVILIVAGACIGYGAVYLPDVLLPESGSA
                     RALYDLFGVPVFWLLALPMPVAFGIAMQRYHLFKADLIINRALVYGTLTACVIGIYAF
                     VVGYLGALLHTEDSFLISLVATSLVAVLFQPLRDRLQRAINRLMYGERDNPYAALSRL
                     GQHLEATLAPDAVLSTVVKTVAEALKAPYAAIEIERNGSYESAAATGEPVDTPLRLPL
                     VYGGERVGRLVLGRRAGEAEFSPADHRLLDDLARQVSLAVHATRLTEDALRLSEDLQK
                     SREQLVTAREEERRRLRRDLHDGLGPQLASLTMKAEAARDLLETSPERSGALLEQITS
                     QTQEAVTDVRRLVYGLRPPALDELGLLGALQAQAAHGDHNGLRVRVEAPEELPPLPAA
                     VEVAAYRIVQEAVTNVIRHAGARNCVVRIAPEAEALRVEVVDDGRGISEDRSAGVGLH
                     SMRERAEELGGSCKVGAAPSGGTSVRAVLPLARGARARQAVEPEVIGKP"
     gene            complement(37409..38032)
                     /locus_tag="VFJ72_11790"
     CDS             complement(37409..38032)
                     /locus_tag="VFJ72_11790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480186.1"
                     /translation="MPSKLIRYGGPAAMLGGVLYIATFGMVYLIYGIFAEQARGTFFG
                     GQEFIYLFYAPMYVFLMLGAVGMYLRQRSYFGLTGRTGFYLTAFGLSLGAIGSAAIVV
                     IGLVSGDEAALAAPIFATHAISHFFYATGSVLLGVATYRAGIFPKEAAVMMGVGPAWQ
                     LALFLSGIDQSYLLLLPPFIITALAWTWLGYALVSEKETPVASAPAV"
     gene            complement(38085..38789)
                     /locus_tag="VFJ72_11795"
     CDS             complement(38085..38789)
                     /locus_tag="VFJ72_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480187.1"
                     /translation="MKNILSKAFGIARGTALTLGVAVMLALTFGLASEAFGANGGSLI
                     LGKATNVATRVTGVVGKVASGAALLVSNPSGGPALDLRVSAGKAPMKVNSATKVANLN
                     SDKLDGVDSTGFLTSTGKAADADKLDGQDSSKFLPAEIYEKSVTQTIPAGSSDGVTTS
                     CDTGDVAVSGSYAIARAGAYLKVFSEQRVDERFDAETGQVNPGGWHVILDNPDTTASG
                     TLTVYVNCADFPPTRG"
     gene            complement(38839..41229)
                     /locus_tag="VFJ72_11800"
     CDS             complement(38839..41229)
                     /locus_tag="VFJ72_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7480188.1"
                     /translation="MTILSGTQRVAGGTTLALGVAVMLAFSVLVTTLPDAAAREARPA
                     GATAGGDHPTFKARPDREAIPGQIIVKFKKDVRPAAQADARRDEGLEKVENLDLIDAE
                     VNEVSGQPVEQAIRDLENRPDVEYAEKDYRLYPTAYADEPRFSELWGLHNTGQVINGS
                     TGVSNVDINGLEAAAVTQGDPNLVVAVIDDGVDFSHPDLAGRQWNSPGEIPGNGVDDD
                     ANGKVDDVNGWDVCHQDATVHDPGDDSHGTHVAGTIAASVNNQGITGVAPNVKIMALK
                     FLSNAGTGPGCNSTSGAIEAIQYAKSEGAKISNNSWGGGDFSQALKDAIEASGSLFVA
                     AAGNGGADGVGDNNDTVPMYPASYNSSNILSVAAVNNRGGLGGFSNYGATSVDISAPG
                     VDILSSVPGSPGTPAAALSSVGTSGKAITAGFGDEEIGTAAGRASFMTKVFQAIGRGS
                     QQVILVDDDRSHDSNADVGPTIAAAIQTATGIAPQTIPVGDGDGPSLAQLSGKTVVWA
                     TGETFYSGFDADFNIIPALTPADLTTLTNFLNGGGKLVITGRDALYQNETSVFVTGTL
                     ALSVNPDIFTNTFTGAQGTAFEGESYTFDGALADPFGISHDGISPAGASARTQGGYDA
                     VPASWESWNGTSMATPHVTGVAALAASVNPALTGNPVALKNLIMNTGKPVSLTAGKTV
                     TGDMADARAAVAAATPDTIKPTISGMSPRLGSIIRDTTPTIKAIVKDNRPLGKANIKL
                     YVNGVLISSTKYSYAASTGSLVYNSPRLAKGKKTVKIVATDAAGNVGAKSWYFTIR"
     gene            complement(41296..42867)
                     /locus_tag="VFJ72_11805"
     CDS             complement(41296..42867)
                     /locus_tag="VFJ72_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480189.1"
                     /translation="MTKTGQIKLFMMATAAALALGLLALLAAKPAEAAFPGNNGKIAF
                     QSDRDGPVEIYAITPGETATRITTSNNSSDPAYSPDGGRIAFLSGNQIFVMNADGSGR
                     RQVTTSSPAKTEPAWSADGTKIAYVSNSFDLDGQTDLEIWTINADGTGRKQLTGNTFP
                     DTQPAWSPDGTKIAFVSARTGDTDRNVYVMNSDGTGQASITPNSPAGCSPNCYQGHDD
                     SPAWSPDGSKIAYVHGYGPPTNPFAGGGVPNIWTMDPAGANKTNVSNNPDTSATQPAW
                     SPDGTQIAYVGVAPLSTDRNIYVMNSDGTGQGPIDTSTAHDINPDWQPVPQCTNTNAT
                     TGNDVITGTSGKDVLCGGDGNDVINGAGGNDIILGEAGNDTLVGSLGNDIVNGGSGLD
                     RASYANSATPVRVSLTTNFATGEGSDVFLGIENLTGSNFSDSLIGSSLANLISGVGGA
                     DSIQGVLGNDTLSGGLGNDTVVGGAGSDRHLGDSGNDTLNSRDGVNGNDTLNGGTGTD
                     RCVTDTREASIRACP"
     gene            complement(42952..45078)
                     /locus_tag="VFJ72_11810"
     CDS             complement(42952..45078)
                     /locus_tag="VFJ72_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480190.1"
                     /translation="MLVKSFTGLENARRLAVAAALLFVLGLALAAAPAKAVPLPCDTC
                     DPGANNPPEVAADNAIVTVNEGQTAVNTGTYSDPDGNATVTLSASVGTVTKDDATGAW
                     NWSFNTNDGPDQSQTVTITAADGTDTSTTTFSLTVNNVAPTVNLQNSFPSVPEGGSFA
                     LGYSSYGYNLSVSDPGNDTWTLNTGCGSNGSEWQESSSTVWCGFFDGPATGTVSATAT
                     DSDGASRTATVNVTITNRNPVAALDAPASINEGGTATISLTKGNFVSPQDGQTDTTAG
                     FHYAFDCNGGSLDGATYASSGTATSTDCAFDDNGAKTVRARIIDKDGGFSEYTKTISV
                     NNVAPTATFEAPASTDQGSNFTISLTSVAEPSSADRDTLSYTFDCGDGLGYVPSTTAS
                     KSCTALDQPSLTVKGKVTDKDGGTNAYTKTVSVNNVAPTGTIKVNNDAAATNNATVSL
                     ALSATDPLPGSGVGHMRFRNENTDTWSAWEPFATSRSWLLSAGDGTKTVHVEYRDNAG
                     NVSTAAIQDDIQLDSQLDTTAPDTAISSGPPALTNSTTASFGFSSDETGASFECKLDG
                     GDFAACASPKGYSGLSNGSHTFSVRAKDAAGNVDATSAVRTWTVDAIRPTISGMSPRQ
                     GSVIRDTTPTIKATVKDNRPLGKANIKLYVAGKLISPTKFSYTASTGSLVYNSPRLTK
                     GKKTVKIVATDPAGNVGSKSWSFTIK"
     gene            45335..45853
                     /locus_tag="VFJ72_11815"
     CDS             45335..45853
                     /locus_tag="VFJ72_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480191.1"
                     /translation="MTTSGIEESAARLLEAFYDLSRGRLKEPVRLRKSDSPRAEGAAQ
                     RAGMDPTSTEPDVAVRYLLNQGYVEATGTNAAYAITVPGMDLVRQRRGLEDPPSERKK
                     MSDKRQRQLVTALAIIIAMVLTRPITRFIDEEIPERRGIKDDLLEAALQGLVRAAAIF
                     AASAIVRKLASR"
     gene            complement(45861..46328)
                     /locus_tag="VFJ72_11820"
     CDS             complement(45861..46328)
                     /locus_tag="VFJ72_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480192.1"
                     /translation="MNGIEATRRITGASPHINVLVVTMFEDEDSVFAAMRAGARGYLL
                     KDASQDEVLRSIRSVAGGQAIFGPAIARRLMDFFAAPQSTVPPRAFPQLTEREEEILN
                     LIAQGHGNADIAGMLYLSLKTVRNHVSNIFTKLQVADRAQAVIRAREAGLGKE"
     gene            complement(46316..46549)
                     /locus_tag="VFJ72_11825"
     CDS             complement(46316..46549)
                     /locus_tag="VFJ72_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480193.1"
                     /translation="MRANLADTGLSAGFYAVYRIALEVGFAVACGALAAGIFWRRSDE
                     RMALFVALLLVLLGTTFWNTIGALALYNPVSTA"
     gene            47270..47410
                     /locus_tag="VFJ72_11830"
     CDS             47270..47410
                     /locus_tag="VFJ72_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480194.1"
                     /translation="MATSNTSGAAIRHSPATIRRKLSASIVLDISASFFEGDQLALYS
                     TL"
     gene            complement(47552..48934)
                     /locus_tag="VFJ72_11835"
     CDS             complement(47552..48934)
                     /locus_tag="VFJ72_11835"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7480195.1"
                     /translation="MTDFPKTLSDLSRALRERRVSPVEVVEALLGRIEADETNAFITV
                     VGEQAMEGARRAEREITAGDYRGPLHGVPVALKDLIFTKGVRTTMGSTFFEDYVPDYS
                     ATVAEKLKEAGAILIGKTNTHEFAYGPTGDRSRFGPTRNPHDLTRMTGGSSGGSGAAV
                     AADLCYGALGSDTGGSIRIPAALCGVVGMKPTFGRVSKHGVFPLAWTLDHVGPLTRTV
                     EDNALMLSVLAGYDPRDPYSADRPVEDFTRDLDGGMRGAVIGIPSSFYFEHIETETEA
                     RVREAAEVFRELGAEVREVEIPNLWDTLKAQRLTLAAEAYAIHEERVKTAPERFDEEG
                     RERLMDGEPLRAYQYANAQQRRIESVREFDRALKEVDALLTTTVPIPAPKIGERETNI
                     GGYEERVRSALTRLTGPTDLNGFPSLSVPCGSTASGLPVGFQLIVRRFEEAAVYRYGY
                     AYERAVRQLH"
     gene            49026..50279
                     /locus_tag="VFJ72_11840"
     CDS             49026..50279
                     /locus_tag="VFJ72_11840"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480196.1"
                     /translation="MLSRDPGEAPRKFLRGAKGTFAAFDRRVWLLLAAMLVFRFGQGL
                     YYPFSTIYFHNVIGIPLSLVGVGLSCLAVSSVAAGFVSGPLTDRYGRKPVMLASLSGS
                     AATFLAFTLVDGFAGYLTVSVLAGLFGSSSFDAARNAMVADVTPLGLRSRAYGLVRVG
                     GNVGWGLGPMVAGLVSAAAGGAGWIYPFLFAGTAALTALVMLSLAVLARESLPAVAMR
                     SPSSGRFSGRRVALSDSPFLVLLGVGVLMYYVFTQDWQALPIYAKNFLGVPDGKIGFF
                     LGANGLVVILLQLPISYLIDRSSKVLALLAGAALFGASSATLLLTDSFIGILLAFAGF
                     FTLAEMVLEVAGPALAADLAPVKLRGTYLGLFGSCFGLSYGISPLVAGPLLQAGLPHA
                     VWTIQLAAVALAAAGLIALATLRRR"
     gene            complement(50311..52452)
                     /locus_tag="VFJ72_11845"
     CDS             complement(50311..52452)
                     /locus_tag="VFJ72_11845"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7480197.1"
                     /translation="METSSSKVWDGDAPERRASDKLRRLLDRAVSASSNGIVITDPKL
                     PDNPIVYVNPAFERISGYPAEEARGRNCRFLQGEDRDQPALEEMREAIREGRECRVVL
                     KNYRKDGTPFWNELYVSPVYGEEGLLTNFVGVQNDITGRKRIEEVLRESEERFRATFE
                     HAAVGAAQVGIDGRWLRVNRRLCEIVGYSREELLERTFQDVTHPEDLAEDLTHVERML
                     RGEIQTYTLEKRYIRRDGSSVWINLTVSLVRDTAGEPKYFIAVIEDISDRKRAEEERD
                     LLLVREQLARAEAVAARRRLSLLAAAGPTLSASLDYEGTLQRVARLVVPELADWCLLD
                     VIGEDGEVEQLACAHADPEKEDLLVRLREHRSFGEDDPGSTARVLRTGKSVLVAEVPE
                     VYRRGGGLTGEHLDIFRQLAPQSIMSVPLLARGRIIGAMTLASSDPVHRYDEEDLALA
                     ESLAYRCALASDNSRLYSERSEIARTLQRSLLPSNLPEIPGVEIGAEYLPVGEANEVG
                     GDFYDVIDVPNDGYICTIGDVRGKGAGAAAVTALARYTIRAVTMRTDLPSEVLAALNE
                     AMLRQLPEERFCTVACIRLEPRDGAPGIGVRVSRAGHPPPLVVREDHSIEEVGCPGKA
                     LGVFDDPGLVDASLRLMPGEVLVMYTDGVTEARSPEGGFFGEERLRHIARSHAGSYAA
                     DIAWKIKHAVLEFQEGVPRDDLAILVLRVPE"
     gene            52580..53209
                     /locus_tag="VFJ72_11850"
     CDS             52580..53209
                     /locus_tag="VFJ72_11850"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7480198.1"
                     /translation="MAYELPPLPYDYNALEPTIDEQTMHLHHEKHHNTYVTNLNAALE
                     KHPDADKGSLEELLANLDSVPEDIRTAVRNNAGGHSNHSMFWQIMGPSGQGGGSPSGD
                     LGAAIDSSFGSFDQFKEQFAAAAAPGALFGSGWAWLIADPSGSLSIETTPNQDTPLME
                     GKTPVLGLDVWEHAYYLNYQNRRPDYINAWWDVVNWDEVARRYDAARNA"
     gene            53390..54778
                     /locus_tag="VFJ72_11855"
     CDS             53390..54778
                     /locus_tag="VFJ72_11855"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7480199.1"
                     /translation="MRLVIIGGVAAGTKAASRARRMDPDLEITLYQEEPEVSISECGL
                     PYYLSGMVNARERLVARTPEKFAAKDIEVLTRHRVEKIDPESRKLTVRDLTTDNTFED
                     TYDRLIIATGARAVLPPISGAGLDGVFKLRFLTDADEILRYLERHSPKKAVIVGGGYI
                     GLELAENLCALGLEVSLIEGADRVALAYGPEVSARVEDHLREKGVKIYTGENVEEFTC
                     EGDREGCVKTVRFGETGIDTDLVVVGIGVKPSVELAQEAGGEIGETGAIKVDRHMKTN
                     LPDVWAAGDCVETTNLASGKPAWVPLGDTANQMGRVAGTNAVTGQDTLEFPGVLGTGI
                     FKVFDLGVAKTGLSEKEAADAGFDTVSATVEATDRAGYYPGVKKVFLNLIADRATGRI
                     LGAESAGSGADKLVDICATAIWGRLSYPDLVNIDLAYSPPYGPALSPVIQAATILSGK
                     FERAREEVRASE"
     gene            complement(54785..56335)
                     /gene="pruA"
                     /locus_tag="VFJ72_11860"
     CDS             complement(54785..56335)
                     /gene="pruA"
                     /locus_tag="VFJ72_11860"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HET7480200.1"
                     /translation="MGLIPYKNETYLDWSDEKNVSAMQAALKDVEGQLGKSYPLVIGG
                     KRLETDGEISSVNPANPSQVVGKLAAATEREADMALDMATGAFEYWSRTAPEARARIM
                     LRASAIMKRRKFEFLAWEVYEGGKPWNEADAQVAEAIDFLEYYAREMLRLKDGVEIYA
                     IPGEESRYFYQPMGVGVIIAPWNFPTAILTGMSSAALVTGNTVIMKPSEFTSVVGAKL
                     MEVYEEAGVPEGVVNFLPGYGSEIGDYLVSDARTRFISFTGSMKTGLRINELAAKQIE
                     NQRWVKRVIAEMGGKDAMIIDDSADLEAAANDIVKSAYGYGGQKCSAASRAILHRDIY
                     DEVLRKVVEKAGALKIGVPESPDVNMGPVISEPQFEKVSSYIDVGKSEGERVLGEDPG
                     DPDKGYFVTPTVFEVDPKARIAQEEIFGPVLSVIGARDFEDALRIANDNPYGLTGGVY
                     SKNRDNLERARQEFRAGNLYFNRGITGALVGVQPFGGFGMSGTDSKAGGPDYLPLHML
                     ARTVVERF"
     gene            complement(56354..57271)
                     /locus_tag="VFJ72_11865"
     CDS             complement(56354..57271)
                     /locus_tag="VFJ72_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET7480201.1"
                     /translation="MGFIDKAIAGSVPVIPRPVVRRISSRYIAGDTLDEAVSTVRDLN
                     KGGCVATIDLLGESTESKADAAATLRDYKKVIDAVEEYGLDSGISVKPTAFGLTINEE
                     LCRANLEEIVEYAGARGRFVRVDMEDSPYTSRTLDMVHTMHERHKNTGAVIQAYMRRS
                     IEDVQPLIESGISVRLCKGIYDEPRKVAYKDFATVRQNYIFLLDELFKGGCYVGIATH
                     DEFLIWHALRLIHQLEVPKDRYEFQMLLGVDEELQSILVNAGHKLRIYVPFGEDWYEY
                     STRRLKENPKIAGYVAKDVIGSITGAIKR"
     gene            complement(57403..58839)
                     /locus_tag="VFJ72_11870"
     CDS             complement(57403..58839)
                     /locus_tag="VFJ72_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET7480202.1"
                     /translation="MAEATGTTQQGAQAEDLKKQLKMRHMTMISLGGVIGAGLFVGSG
                     TVIGAVGPAAVLSYLMAGIILLLVMRMLGEMAVTRPVVGSFAEYARMALGGWAGFTIG
                     WLYWFFWVVVVAVEAVAGAGILETFIPGVPLWLFCLILVSLLTATNLYSVGAFGEFEF
                     WFASIKVVAIITFLALGTIFVLGLWPGASLDFSNLTSHGGFFPNGPFAAFNGIVTVIF
                     AFVGAEIVTIAAAESAEPGRGVARAVNTVIYRVMIFYVGSIFLVVTILPWNANKALGR
                     GPYSAVLDHIGIPGASTIMALVVLTAVLSCLNSGLYTASRMLNSLSRRGDAPKFFLDV
                     TRRGVPAKAILISTSVGFLAVIAAYVSPETVFQFLLNASGAIALFVYLLVALSELILR
                     RRFEREAPERMQLKMWLYPYLTIFTIVVFVAIIISMGIIPDTRSQLALSLLSAGIVVA
                     AYFLIRRRFGGGQQQTVDATSPSDRGER"
     gene            complement(59134..59841)
                     /locus_tag="VFJ72_11875"
     CDS             complement(59134..59841)
                     /locus_tag="VFJ72_11875"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7480203.1"
                     /translation="MELGEIAARASGKFRTTQSMVASGLRDAILSGVLAGGQPLRQEE
                     IAESFGVSRSPVREALRQLEGEGMVTFIPHRGAVVSELSYHEVEEITEIRVALETMAM
                     SKALPRLEDDDLERAAEILRRIDREEDLITYWSELNWRFHATLFAPAERPRLLALIKA
                     QHDAFERYIRMHLALSEYEKPQREHYELLELCRKKDTDAVLNLLTRHIEETSELLFTY
                     IKENSGPDAKEIHKDHA"
     gene            59937..>60508
                     /locus_tag="VFJ72_11880"
     CDS             59937..>60508
                     /locus_tag="VFJ72_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7480204.1"
                     /translation="MQKTDIQRGPASKTRTVASLIGGRTLEDAPGGRMTSINPAHTDE
                     RVAEVLLGDAGTFVAACRMAREAQREWAAVPAPIRAQVIKKIGRLVESNKEALARLVT
                     REIGKPYTEALGEVQEIIDTCDFFTGEGRRLYGQTVPSEMPDKQLFTFRVPVGVAAII
                     TAGNFPVAVPSWYLIPALTCGNTVVWKPAE"
BASE COUNT        10647 a        19846 c        19286 g        10729 t
ORIGIN      
        1 gctcacagga cgatattatc cgacgaatac acgctcctgc accgctactg cacgtaatta
       61 ccggcaagtc gaagcaatca atgcctgtca gagtggcgct gttcacaatg gagactcgac
      121 gctatatgct cgctgcatat actctggccc gcactatcga agtcacatta ctttagagac
      181 ggaggcgata ctcgatgaag gaagcgcttt acggcatatc gttgcgcaaa acggagaagt
      241 atgaggatct cgcgcggcgg gctcaggagt ccgacgacca ggagctggcc gagttcttcg
      301 accggatagg cgaggaggac catcggctgg cagaggaagc ccggcggctg ctcgcccagc
      361 gggtggccga gtaacaccga agagtcacag agggaggtca agaggcatga ggatagcatt
      421 catcggcccg cccggagcgg gcaagagcac ccaggcgaag cggctcgcgc ggtcgcttcc
      481 ccactggaac acgagtccca ggctctccac cggggagctg gtgcgagcgc agatagaggc
      541 ggggacggat ctgggccgcg agttggagga gcggtatgag cgtggtgaga gggtcccgaa
      601 cgagacgatc ctctcgctcg tgctgccgcg ggtgaggagg tctggaggtt ggatcctgga
      661 caacttcccc gctgatgtag ctcaggcgaa ggcgctggac gaagagatcg aagagcgcgg
      721 cgccgggggt atctcccgcg tcatctccct ggaaggtcca tccgaggagg agttgatccg
      781 gcgcgtcctc ggcggccggg tcaccagccg ggccacgggc gaggtctacc acctccagaa
      841 cgacccgccg cccgaagagc gcgagagaca ggaccccggc cccttcgaaa agcgcggcga
      901 tgatacggaa gagtccctga agaggaacat ggtagcctac cggcgcgagg ccgcagcgct
      961 caaggaatac tacgaagcgc ggggcgtgct ctccgtcgtg gacgcccgga gatccatgga
     1021 cgaagtagcc gaggaggtcc tggaagctct cggacatccc gagcggtcag agttctacgc
     1081 agccctgcgt aaaagcaaga cagcgtagag agagctgctg gatcctcccg tcatcgagga
     1141 ggttcgacgc gggagatccg ccagtaaacc accccgcgtc cgcgcgtcac agcatcccga
     1201 acgtcgaggc gcggcgcggg tagcgggaga cgccgctcca ccacgtcttc ggcgaggcgc
     1261 cggtaggtgt ccagccccgc cggcgtctcg ccgataccgg cggtcagcgc caccagcgcc
     1321 gccgtcccga cgccggtctc gtaggccgag cttatgaccg gccgggcgcc gatctctcgc
     1381 gcccggctcg ccatccgcag cgcttgcgag atgccgccga gcagggtcgg cttgatcacg
     1441 accgcgcccg cgtaaccgtg ctccaccagc atctcgggag ccataccggc cagcgactcg
     1501 tccagcgcga ccggaatacc acagacgctt gcgaattcct ttagaccatc cgggtccgcg
     1561 agcggctctt cgatgtactc gatccccacc ccctctacgc cgcgagcgaa ccgcaaaacc
     1621 tcctcgaaat cccaggcccg gttcgcgtcc agcctcagcg aaacactctc accaattacg
     1681 cttcgtacct cgcgcacgag actaatatct tcctccaccg aaagtccgcc caccttcagc
     1741 tttatggcct cgtaaccggc gtcttgcatc cggcgagcct cttgcaggac ttcggcgcgc
     1801 ggtccggcaa gcagcccgtt cacggacacg atgtccctcg gacttgccga gaccaactct
     1861 ggcagcgtca cctcgctggc cgcggcatac aggttccagg cggccgcctc aagcccgaag
     1921 cgtacggagg gagcggggtt cgtgtcatcc agctcgcggg cgagagtgcc gtccgcgcgg
     1981 gtccaggcgt cctccatatc ccggccaacc atcgccgccg ccaaccggcg cagctcgcca
     2041 agtgcggcgc ccaggctctc gcggctgaag ccggggagcg gcgaggtctc gccccatccc
     2101 acggcgccgt cctcgccggt cagccgcagc aggagcccct cacgccggcg taacgtcgcg
     2161 cccttcaaca tgagcggtcc ggtaagcggc aggctgtagc ggtagagatc cgcgccggcg
     2221 atcctcacag tgccggtcta ccgccgggct tacgggcggc ggggaaactt cgagaaatcc
     2281 ggcttgcgtt tctccttgta ggcgtcgcgg ccttcctgag cctcctcgct cccgtagaag
     2341 aggaggttcg cgtcgtgggc gagctgctgt atgccggcga acccgtcctc gtgcgcgtgg
     2401 aagctcgcct tcaacatccg cagcgcgaac ggcgagcggt ccagcatctc ccggcaccag
     2461 cttacggtct cggcctcgag ctccgcgagc ggcacgacct tgttgacaag acccatctcg
     2521 taagcctcct cggccgagta catccggcac aggaaccaca tctccttcgc ccgcttcggc
     2581 ccgacaagct gcgtgagcac cgaggcgccg aagccgccgt cgaagctgcc gaccttcggg
     2641 cccacctgtc cgaacttcgc gttatccgcc gcgatggtaa ggtcgcagat aacgtgcaga
     2701 acgtgccccc cgccgaccgc ccatccggcg accatcgcca ctacgggctt ggggcaacga
     2761 cggatctcca catgcaggtc cgtaacgtga aaacgcccga ccgacgcgcc gccgggggtg
     2821 cgccgcgggt cggtcgggtc ctccaggtat cccgcgtccc cgcgcacctt ctggtccccg
     2881 ccggagcaga aagcctccgg cccctcaccg gtcaggatga tcaccccgac ctcgtggtcc
     2941 tcgcgggcgt cttcgagcgc ccgctgcatc tcgatgaccg tcagcggacg gaaggcgtta
     3001 cgcacctcgg ggcggtttat ggtgatcttc gcgatcccct ccgccttctc gtatttgatg
     3061 tcctcgtact ctccggctgg ttcccaagtt atgttagtca tggaatggat tatagatatc
     3121 tgacagaaaa cctttcagta aagtcgtgta cgcttcgcaa gcttcgaggc gcacgttgtg
     3181 ccccgcgcag ggaacaatag cggtccttac ctgtggattc gactcccgca ttcggtgcga
     3241 gatcccggcg aacttcccgt ccagctctcc cgcgacagcg agcatcggaa cgtcgagtcc
     3301 ggcaagctct ttccacagcg acggctggct gcccgtcccc atcccccgca aggacttcgc
     3361 aagctctcgc gggtcgttgc tcctccgcgc ctcgatagtg cgccggagca gctctttatc
     3421 ccgcgctagc gacgcgaata gtggctggag ataccaatcc cgaagaaact cgtcgaagtc
     3481 tcccgtctcc aggcgcgcgg cgcgcctctc gtccgcgatc cggcgctccg tccgcgcttc
     3541 ctcactctcc agtcccggcg aagcggattc cagtaagagc ccgccgtacc gctccgggta
     3601 gcgcagcgcg aggtagagcg ccagccgccc gcccatcgag tagcctacga aagccgcccg
     3661 gtctatctct agctcgtcca gcacgtccag taccattcgc gcggcgctct caaaggcgta
     3721 cgaccctgga agcaggccga ccgaaccacc atgtcccggc aggtccacgg atacggagaa
     3781 gaacctgtct tcgagggccg tcattacggc acgccagtct tcggaagctc ccataaagcc
     3841 gtgcaggaag aggaccgggg ggcgtccggg atctccgcgc gactcgtagc gcagccggga
     3901 gccggccggc aggctcaccc ggagagacgg gagcgcagga tcttcttcca tccatcggcc
     3961 agctccggga cttcgagaag ccgtcgtgcc agggccggat cgaagctccc gtgcgcgcac
     4021 tcgttcacga gacgctgggt gatgcccggg ttgggccggt cctcgattcg cagctcatcc
     4081 ccggtacgca ccgtgccctc ttcgaggacg cgcatgtaga aacccgtccg cagcgactcc
     4141 agggtgcgcc tgtggaagcc gggcagccct agcttccgct cctgcttcca gcacgggact
     4201 cgcggtccgc taacctgcac ccgcgaggta ccggccgcgt acacgtcgcc cacgcagatg
     4261 ccgctctcat cgtccccgaa cagggtccag ttctccccca cgccgccggg tcccacagcg
     4321 tcctcgccgg agaggccgta gaactcgttc cagaaagcgt agtgacccag aggatggcag
     4381 cagagggcct gatccggcgt cccgtggttc ttcgtgtccg ctacccggtc tccctccagc
     4441 ccgcgtactg tgaccctcac ctcgccgtct accggagcgc ggaagatcgc ggaccgccag
     4501 gtcccgcgct cgtcggtaat ggtccgcggt tctccggtaa agatagagtt aatcttgatc
     4561 attgcctcaa cataaccccg aggcgacgct gaggcaagcc gctagactcc gccgttcagg
     4621 tctctatgga tctcctccac aagctcccgg tgcagcgcca cgttctcctc ccggtcggtc
     4681 ttcacttcga tcagtgtcga gctcccgcga gcgcaggccg cacgataagc cttcacgaac
     4741 tccgtccctg tgcgcggact ctcgtagccg agcccgaaca tcgtcgccgc cgggccgaac
     4801 cccactccct gcggtgtccc gaagtacggc tcgaagaacc cccggtgtcc cgccaccggc
     4861 aggaaagaga agataccacc gccatcgttg ttcacaacga tcacgaccac cagcgcgtcc
     4921 cggagcatcg ccagcgagtt caggtcatgc agcaacgcaa ggtccccgat gatgagcgta
     4981 acgggtgcgc cacgaccgcg agcgaagccg gcagcggttg cgacggtgcc gtctatgccg
     5041 ctcgcgcccc ggttcgcagc aaccagtacg ccggcgccgt ccgtcaccgc gaaggtgtcg
     5101 aggtcccgca ccggcatact actcgccgcg acgagaccat gatctttggg gacatgccgc
     5161 gagaccaggc gagccacgag cggctcgctc agatcttccg tctccgggag aagcttatca
     5221 aggctccggc tcaccttccc ggaggcttcc cgccatcccg caagccatcc gtgctgcagc
     5281 ggggagactc catcgaatgc cgcgcagaag tctacgatgt ccatctccac gctttgggta
     5341 acgcggtggc cgggatccag ccggaacggg ttctcgcgca cgacgatgta cggctgcggg
     5401 cggctacggg cgacgagctt ttcgagacgt ttcgagagcg cgcgcccccc gatgtgcagc
     5461 acggcttccg gcgcgtgcgc ctccgcgaac gtctcgctcg ctagcaaggc gtcgtagtgg
     5521 gcgaccacgt ttcgggactc cgcgccgagc cgcgcctggg agcccacatc cggcaggagc
     5581 ggccatccca gggcttccgc cagctttatc acggactcgc cctgcgcgcg ggaagtgaga
     5641 cgcccggcga tcaccacccc gcgctctacg gtccgcagct cctcccggat gcgccttacc
     5701 tccgtctcat ccacggcggg cttcgtagcc gcgtagcgcg tgtagggtac attgctctcc
     5761 cgccacgatt ccggtcccgg cggagcttcc gcctcatcgc cagggtccgg gagaaaaggt
     5821 tcgcggaaca tcaggttgag gtggacgggg ccggacgggg agcggcgaga gcggtagacg
     5881 gcctggtcca cggttgtcag gaccatcgcg gggtctatcg ccgggtccgg ggcggggagg
     5941 tcgaagcgcc agcggacgta gtcgccgaag atgtcgggct ggtctatcgt ctggttggcg
     6001 ccggtctggc gcagttcggg cgggcggtcg gcggtcagca ggatcatcgg cgcgccgtcc
     6061 acggaggcct ccaccacggc gggcaggccg tttgctacgg cggtgccgga ggtcgtgatc
     6121 caggccgccg ggctcccggt cgcccgcgcg tatccgagag cggcgaacgc cgtgccgcgt
     6181 tcgtcgaagt gtattagcga acgggcccgg tcgttctcgg cgagcgccgc gacgagcggt
     6241 gtggagcgcg agcccggcgc gacgcagaag agccccacgc cgcagcgcgc gagctcctcg
     6301 acgatcaggg ccgcccagag gcggttaagg tgcggcgtgc tcttcggggt caagcccgaa
     6361 caccctggtg aagtccccga tcttctgctc gatctccgcc cactctcctt ccggagtaga
     6421 tccggcgacg gcccccgccc cagagtagag cgagaccctg ctgccccgca cgagcccgga
     6481 ccgtatcccg acggcgaact cggccccgtc ggccccgatc catccgatag cccccgcgta
     6541 ccatccgcgg tcgaagggtt cgagcttccg tatctcttcc agcgccacgt tcttcgggta
     6601 gccgccgacg gctggcgtcg ggtgcagctt cgcgagcacc tccgcgtccg tgacgccctc
     6661 gcgcagcgtg ccccggatct tcgacaccag atgccgcccc cgggccagct tcatctccga
     6721 cggatggtct tcgatctcca gctccgcgca cagcggcccc agggtctcct cgatgctgat
     6781 ccggacataa gaatgctcgg cctggtcctt cacgctccgc agtagctcgt cccgcaactc
     6841 atcatcgtcg gctacggaag cgccgcgggg cctcgtaccg gccacggcct cgctcgtgat
     6901 cccgcgtccc tcccgccgga agagccgctc cggcgacgcc cccacgaacg ccgctccccc
     6961 ctctggctca accaggaagt gaaagcagcc gggcgtcgcc tcccgcaggc tcgccaccag
     7021 cgcgaccgcg tccacgtcct cctcgaacgc gaactcggcc cggcgcgcga gaacgacctt
     7081 atccatcctc cccgccgcga aagcggcgag cgccgactct atattctccc tccacccctc
     7141 gcggtccggg ctgtcggcgc gcgagacggg cgccggcagg gaaccggcga gccggggccc
     7201 cttcagcggc aggttttcga tctcgtaaag taaatctccg gctttctcaa gatcgcgcgg
     7261 caggacgagg ttgcatgcca gcgtcgaaac gccatcccgg cggcgcaact cgaagcgcgg
     7321 caggacgaag cggcgagctc cgaacacctg ccattcttcg ctcacttcag agcccgcatc
     7381 gaaactcagc ccgccgtagt agcgagcatt cgccgcctcc ggcgacagca gagcgagccg
     7441 atcggtcagc tcatcaacat ccgccggtct ctccccttcc acgacatccg caactccgac
     7501 ggcggccacg ctgatctcgt cctcgcgtcc cgaccagaag gacctcggcc gcatcgtttg
     7561 cgcgccgagc caccgcagcg ggtcaaggtc tccggcgggt acctccagcc gcaagaccct
     7621 ctcaccccgc tggaggccgg tgttcagcgc gagagcccgg ctcacggcgt ccgcaagcgt
     7681 ccaccggacg tcagtcctct cgaatacgga gtccctgata tacctactca gcaagctgga
     7741 gtatccaggg cgtcttgtaa gggatccatt tcatcttgat ccgtagtgta tcaaggtgcg
     7801 tatgaaggtt tagcggcttc gctcacattc cggggcgcgg ctcgtgcagg cggggataga
     7861 tctcgcggta ccgcgcatac gccgcttcgt actcaccact ctcttcaccg ggacgcacga
     7921 cctctcctgt cgtatctccg ggcgtaacaa tatcctccac cctgagtgcg ccggaggcga
     7981 gcccggccag tatcgccgcg ccgcgggcgg aggcgacgga ggcaacattc tcggggagca
     8041 ggcgcagcgg gcggccgagc acgtcggcca ggagttgccg ccagggttta cgggccgttc
     8101 ctccaccggc gagacgcagc tccgggatgc cggcgccggt ctcctccagg gcttcgagac
     8161 cctcgcgcag cgcgaaggcc acgccttcca gcgccgcgcg cagcaggtgg ccgcgggtat
     8221 ggtcgagtcc gagaccggtc caggtcccgc gggcactagg atcgaagcgc ggcgtccgct
     8281 ccccgctgag gtacggcagg aacgcgacgc ctccggctcc cggcggaacg gcaaaggctt
     8341 cctcgtagac ctcatcccag ccagcgccaa gcacgcgacg agcccactcc agcgcgagtc
     8401 cggcgttctg gatggccgcc atcgcgtacc atcccgtatc tgtcgccgcg cggtacaggt
     8461 gcgtccgctc gtgaggatcc ggttcaggac gttctctcgg gaccacgatc tgtccccccg
     8521 tccccacggt gagctgcgcg actccagatc tcatcaatcc gctccccagc atcgccgccg
     8581 ccgtatccgc cgcgccggcc gcgaccggga tttccgcccc gattcccaac gcttccgcgg
     8641 cctctcgcga gagaggcccg gcgacgctcc cggacggtac gagcggagcc agcaactcat
     8701 cccgcagccc gagagcttct accacctcgt aagcccatcc gtcgccttcc aggtcgtaga
     8761 gcagggtagc cgaggcgtcg gacggctcgg cggctgcctc gcctgtcagc cgcaggcgca
     8821 gccagtcctt cggctgcagc gcccaccggg cccgagcata aacatccggc tcgtggtcgc
     8881 gcagccacaa gagactcggc ccggccatcc cggtggcggc ggggttggca agcctccggc
     8941 gcaggtcatc gccgagagcc tgatagacag tgagttgctc cgccgagcgg gtgtcggccc
     9001 agagtatcgc ctgccggaga ggccggcctc cggcttcgga gaggacgacc ccgtgcatct
     9061 ggccggagag accgatcgcc cgcacctccc cgccgcgttc cccgatagcg gtcctggtgg
     9121 cccggatgac gccgttccac cactcttccg gatccgtctc cgcccatccg gagcgcggcg
     9181 ccaggacatc gtacggtgcg gagccttcgc caagtacttc cctctccgag gagagcaaga
     9241 gcgcttttac ggaaccggtg ccgaggtcca ggccgagtag catgccagcg attagaagag
     9301 gcgcgggacc gtcccgcgac ccggccggag tgtgaaccca taaaccacga taccggcaac
     9361 gttagcagcc ccccacgtca caggccaccg aagagcccgg accgaaacgc cgagagggac
     9421 ggagcctgta acttgcgctc cgtccctctc gtgtccatct gccctgcggc gttacgcctt
     9481 gacgtcctcc agttcggcct ggacgatgac ccggttcgag tagtccggga gcgtgcaggc
     9541 ctgcaggctt acgatgttct ttcccttcac cgggttgagc acgtaaaggt cggtcggttt
     9601 gacgaccatc gtcttgaaga ccttgtaggt gtacttctga ccgtcggcgt cggtcaggta
     9661 gatctggtcg ccatccttta gcttgtcgat atcgtagaag gcgagatagc tgccggtgcc
     9721 ggagtaaccg aggcgatgtc ccgcgatgta gacattagcc tccttctccc agggatttcc
     9781 ggtgcccttc aaatgtatcg cggcgttctc ccggagcaac gcctcatccg tgccctcgcc
     9841 aacggggatc ttgcggtcct tgatccgctc catcttgggg atggtgagct tgagggtcgg
     9901 atcggacggt accctggcga tgtcctccac cttcttgccg gacttctcgt cgcccttctt
     9961 cggggccacc tgctggtcct tggcctgctc cgacatcttc ttgtcggcct ggctgtcagc
    10021 ctggccgaac gaaccgcacg cggcgagccc cgccaccatc actagaaccg tcagcatcag
    10081 cgcaaatttc ctcaaaccca cctcacacat caaccctcac tggcctttag taaatcgcaa
    10141 aaccgggacc gcctcacgcg gagacgatcc cggctcgata gcatcctctg cgtccgcctc
    10201 ctatccctag gcctcgacgt ccttcagctc accgcggact atgagcctct cagtgttcga
    10261 gtagtccggg ttgccgttgg cgtccacgtt gacgatctcg cacgtctgaa gagtgatgac
    10321 gttcttgccc ggcacggggt tcagcacgtc tacctcagtc gggtggacga tcttcttctc
    10381 agacaccttg taagtgtact tctggccgtc ggcgtcggtg acgatgatgt catccccctt
    10441 ctccagcgtg ccgatgccgt cgaatgcctt gtagctgggc gtcccctggt agccctcgac
    10501 gtggcccgcg aagtagacgt tcgcctcctt cttccacggc catccggtcg cgaagccacc
    10561 ggatttcgtc gggtaccgga tgtgaatcgc cgcgttgttg gtcaacatct gcgtatcgtc
    10621 accctgccca gtcggaagat ccttgaagtc cttgcccaac ttcgggatgg aaagtccgag
    10681 ggtcttctcc ttcggcacct tcgcaacgag cttcttctca cccttgccgg agtctttttt
    10741 cgcgtcggac ttcttcgcgt cggacttttt agtgtcggac tctttcttgg gagcggcctt
    10801 ctcggacttc ttgtcggaag ccttgctccc ctgatcaccg ccggactgac cggacgagcc
    10861 gcacgcggcg agccccacca ccatcgtcag agccgccacc aacaacacca tcttccttaa
    10921 aaccatctca tcacctcgta tttcggttct caaacgccag agtctcgtcg aagccctaca
    10981 gccccgtttc agcagccagc atcctagcgc ccgaacgtaa catagcggct acaactcgac
    11041 caaaggttta ttacaaactc tctccgatgt cgagggtgcc aacccttgtt caaacctttt
    11101 atcaaccctt atatatcaac gacacaccag cgcgcagtat aaaaatgact cactatctcc
    11161 gcgttagcgg accacatact aaggagattg ccactatttg taaagacttg ctcgattgac
    11221 gccgctctct cagaaaattt ctcagatatc agaacaacgg ccttgacacg ttagaatata
    11281 tgtgtaaact gcacatagac ttcaagaaga ggtctgcatg taggtaaatt gagagagtta
    11341 gaggaggcag agatcatggc tacgatggag aagaccaaca aggctgcgga gcggctggcg
    11401 aaggcgacga gcgactcgta caagacggtc attgaccaca cggtggcgct gcaggagcgg
    11461 aacgtccgct tcgcgcaggg actggtggac ggcgcggctg gcgagcttcg tcaccaggcc
    11521 gagagcaacc ggagcctcgt ggaagagcta gtcgagcggg ccgagaagca gcgcggcgcc
    11581 ttccagaccc tcgtggagga gtcggtggac gcctacatgg acttcgtgta tgcgcccttc
    11641 agctactaca agcagggtct gcggctcatc gagaccgagg tcacgcaggg cgggttcccg
    11701 atcccgaact atgacgacct gaacgttggc gaggtcacca agctcatcga cagtctcacc
    11761 gccgagcaga tccggacggt ccgcgagtac gagaagaaga acaagaaccg tgagaccctc
    11821 atcgagcagt tcgaccgcaa gctcaggccc gcctcgtaag agaatctctt aagagagcct
    11881 cgtaagagga caacagggca caacaggaaa gggccgggat ctcttcccgg ccctttcgct
    11941 tgctcttttt tcgcgtatta tttgagcagg tcttttagat gccccttctt ctccttcatc
    12001 gttcccttgc gctggtcagc gcggccttcg gccttcttgg ttctgtctcc ggtcagggcg
    12061 cccgcggcct ccttcaagcg gcccgcggcc ttatccatct tcccttcgcc cttgtcctga
    12121 ttcgcggtgt tgcgccgcct gctcatcctg tcctccttgt tcccggtagc atttgctact
    12181 tgcgttttac ccgcgggggt cgcgcctaaa ccttctcatc tggactatgg ataggcacgt
    12241 aagtcccgca aacgcggcga ttcccggcat cggggctccg tatcagggct cgacccgtct
    12301 ggaaggtagc taatttagca taggcaaaga tcattgtttt tctagatgtc ggatagaggg
    12361 tcctgatgca tccttgttag tacaacctgt ccaagacaaa ggagtgagtt gatgaacgca
    12421 atgatctcgg agcacaagga gacgctggcg aagatgctgg tggccctgtt cctggcggcg
    12481 tctctgctga tagcggccgt cggtccggcg gatgcggcag cgaggtccaa gtgcagaaac
    12541 aacccggaga acttcagctt cttccacggc aagtgcctct ccgacaagag gatcgagaag
    12601 ctgacgggcg gcgagtagac cagtaaggtc gcgtatcttc tagaagagcc ggggatcgat
    12661 cccggctctt ttttacgtgt tctctccggc atcccaggca accttttcca ggcagccttt
    12721 caacagattt ctctcccgat aaatgctaaa aggtactgta tcgtaggcac tatcaactag
    12781 agggagagaa tcgtgaacgt agctgccatg acccgcgtgg ctctgatggt cgctgtaaca
    12841 gccgccgccg cgcagatagc gataccgtta cccttctctc ccgtaccgtt cacgttgcag
    12901 gtgcttgccg tgatcctctc cggcctgtta ctcggcccgc gctacggggc tctttcgcag
    12961 gcgctatacg tgctcgtagg gtctatcggt gtgccggtct tcgcccagtt ccacggcggc
    13021 ctcggtgtcg tcctcggtcc tacgggcgga tacctcctct cctatccctt cgccgccgcc
    13081 attgccggtc tcgccgccca cgccgccgcc aactctcccc gccgccgcgc gctgacccgg
    13141 agcttcgtct ggggaacgat cgggctagct gtcatctacg ctcttggcgc ggcatggctc
    13201 tccgtggtcg cggacctgcc ggtagcggcg gccgtggcgc agggagtcct gccgttcgtg
    13261 gtcttcgacg tgatcaaggt aggtctcgcc gcactcgtgg cggtagccgc cgcgccagcc
    13321 atcgccacgt cgcgggttta aagatcccgg aagaaccagg gctcgcgtcc tttaacggat
    13381 actactattg tcgccgcctc gactacgaac cctttatgcc accgagtcga aggtgcgcgc
    13441 caggatcttc agcccctcct ccagctcgtc gtccgcgatg ttgagcggca tgagggtgcg
    13501 gatgacgttg ccgtactggc cggcggtgag gaggagcagc ccttcctgaa gcgccgtctc
    13561 cacgaccttg ccggtgcgct ccttgtccgg ctcgcgggtt ccagcgtcct tgacgagctc
    13621 catcgccgcc atcgcgcccc gcccgcgcac ttcgccgacg aagtcggggt gcttctgctg
    13681 gatgtcgcgc atcgcgccca tcacgcgctc gccgagggcg ttcgccttgc cgaggatgtc
    13741 actctcctcg aactcctcta tgacggcgag ggccgcggcg caggcgagcg ggttgccgcc
    13801 gtaggtggtg ccgaggccgc cggtgtggac ggcgtccatg atctccgcct tccccgttac
    13861 gccgccgatt ggcattccgc cgccgaggct cttggcagtc gtcactatgt ccgggatgac
    13921 gccggagtgc tcgatggcga acatcttgcc cgtccgtccg aagcccgtct ggacttcgtc
    13981 cacgatcagg acgataccgt gctcgtcgca caactccctt agctgccgca ggtagaagtc
    14041 cgggaacggg atgaatccgc cctcgccgga gacgggctcg ccgattatcg ccgcgacgct
    14101 gctggggtgc acgtagctca cgaaagcctt ctccagagcg ttgaagcaat cgccctggca
    14161 gccgccggag cagtccttgc ccgccgggca ccggtacggg tacggggcgg ggacacggta
    14221 gatctccggt acgaacggcc cgaagctctt cttgtacggg tcggtcttgc tcgttagcga
    14281 catcgcgagc atcgtgcggc cgtggaaggc gttctcgaag gctataaccg ccggacggcc
    14341 ggtgtaggcg cgagcgatct tgacggcgtt ctccaccgcc tccgccccgg agttgatgaa
    14401 catgctgcgc ttcttgaagt cgcccggcac cagggcgttc agtttctcgg ccagctctat
    14461 gtagggctcg tactcggtga cgtagaagca ggtgtgcaga aagcggtccg cctgctcctt
    14521 gaccgcctcg acgacgcggg cgttcgtgtg cccgacgttc aacacgccga tgccgccgcc
    14581 gaaatctatg aacgtgttgc cgtcgacgtc ggtgaggagc gcgcccctcg cctcctgcac
    14641 gaacatcggg gtggcgatcc cgatgcccga cgtgaccgcc ctgtgccgcc gctccatcag
    14701 cgcccggctc ttgggacccg gtatctccgt ctttatcctc gtggactcca gcatctcagc
    14761 cacctcttcc tgtcggcctt actcgttcta gtgtactcgt tgtcgcgccc tacgcgatac
    14821 ccccgagaga gatgtacttc acgtccaggt acatctctcc catgatgact tccctcgcgc
    14881 ccgaagccgc tctccgccga gggctacctc cacgccctcc tccagcccgt caccgacctt
    14941 catcgcggcc tgcgtcggtc agggtagtca ggacctcgcc gttcgcgggg tttgtcacgt
    15001 cgaaggtctt gccggaggcg attcgctccg tccactcgcc accgacgaag agcgtctcga
    15061 tcactgtttt tctactctcg aatgtgcgat cagactcaaa aactcatgcg cgatgttggc
    15121 ggccagaagc gccgttatct gcgccgggtc gtactgcgga tagacctcca ccacgtcgca
    15181 gccgacgatg ttcagaccgg cgaggccccg cgcgaactcc tgcgcctcgc ggctggtgaa
    15241 acccccgatc tccggcgtcc ccgtgccggg ggcgaaggca gggtccacga agtccacgtc
    15301 gaaggagacg tagatcctcg catcgtccac ccgctctcgg atgcgctcta tcgtctcggg
    15361 gatgccgatc tttcttacag cgtccgtcgg aacaagctcg aaacccatct gccgcgcgtc
    15421 atcccagtcg cgctcgtcgt agatagaacc ccgcatcccc acctggatcg aacgctccgg
    15481 tctcaacagc ccctcttcca cagcgcggcg gaaaggcgta ccgtgagtgt gcttctgccc
    15541 gaagtaggcg tcccaggtat ccgggtgcga gtcgaactgc acgagagcga gcggtccgtg
    15601 gacggcagcg gcggcccgaa gctccggcag cgcgatggag tggtcgccgc cgagcactac
    15661 gggtatgacg ccggctcggt ggatggtctc caacccttcc gcgatcttct ggtagctggc
    15721 ctcgatatag ccgggaacca caggcacgtc gccgtagtct atgaccgaga ggtgatcgaa
    15781 gatgccgaca cccaggctgg ggttgtaccg ccgcaggagg tgcgagacgg cgcgtatccc
    15841 ctcgggccca aagcgggctc cggcgcggaa ggtggcgcct gtatcgaaag gcgctccaac
    15901 gatggcgacg tcggcgtttt cgagatcctg cgtgttcggc aagcgcatga aggtgcgcac
    15961 gccggagaag cgcggcgtct cgaaagagtc tgccggttcg tagctggtca cgggcatcta
    16021 ccggcccgcg ctcggagcat ccgctctcgc cgctctccgg cgaattttcc gcgacacgac
    16081 cggatgatct cgccccagcg cacgtctcca ggcggggaga tacccgctta gtgtaaaggt
    16141 aagagaggca accgctttga gcttcatccc ccgccctgcc gggaaccgtg acggccgcgc
    16201 gattccctcg ggctgcggac caccatgacc ggacagttcg cgtgccgcaa tacgctgtcc
    16261 gaagtgctac ccaggagccg cctcagggga ccggagcccc tgacccccat gattatcagg
    16321 tgagagtcca actcgtcgct cagggcgatg atctcctcgt ccgcattgcc cagcctcaga
    16381 tgggcctgcg cgacggtcgc gccggtctct tcgatcctct ttacttcctc atccagtatc
    16441 tcctgggcgg ctttcttaac ctcgtcgtac agctcctcgt agatacgctg accagcggcg
    16501 aacgctacca ccggctccac gaacacgacg tgcaactcag agccggtctt ctccgcgatc
    16561 tcacccgcca cccgcgcagc taatgccgca gtgtcggagt cgtcgctcgc tatgagtatc
    16621 tttgttggga acatggtgtc catctgtttt cctcctctct cgccagtttt ccccgcgagg
    16681 caaaatggac ggctctggcc cgtgatgccg gacgctcaac ccgcctgaac cgcagtggct
    16741 cgttgcgctc gcgcacatct gcggcgtccg ccaaaccacc tctccttccc gacgtgatca
    16801 tatacccgcg cccccttacg ccgcaccccg aacgatccat cggagtatca gggacgtgtc
    16861 tgctatctaa ggagcttcgc ggggtccatg taggacagac cgtgggcctc ggcggtcggc
    16921 tcgtggacga ggttgccgtc cagcgtgctc aggcccttcg ccagcgccgg gtcagccgcc
    16981 gcggctcctt ctatcccctg atcggctatc ctgatcaggt acggcagcgt cacgctcgtg
    17041 agcgccagcg tggaagtcct cgcaaccgcg ccggggatgt tcgccacgca gtaatgcact
    17101 actccttctt ctatataggt cgggtccgag tgcgtcgtcg gacgcgccgt ctccacgcac
    17161 ccgccctggt ctatagccac gtccacgacc accgagccgt ccctcatgct cgccaccatg
    17221 tccctggtca cgagcttggg cgccttcgcc ccgtgtacca gcaccgctcc tatgaccacg
    17281 tcggattcgg cgacgtaaga ggcggtgcgc gcgcggttcg ggatcaccag gtccacccgc
    17341 ccctcgaaga tgtcggagag gtacgcgagc cgcttggggt tcacgtccag caccctgacg
    17401 atggcccgca tcccgaccgc tatcttcgcc gcctccgtcc cgaccacccc gccgccgatg
    17461 atcgtaacct tcgccgccgg agtgccgggg acgcctccga gcagcagtcc cgcgccaccc
    17521 tgcgggctct ccaggcagtg ggccgcggcc tgcgtcgcca tccttccggc cacctcgctc
    17581 atcggtgtga ggagcggcag gctcccatct ggtgtctcga ccgtctcgta ggctatcgag
    17641 ttgaccttct tctccatcag gaactcggtg agtcccttgt ccgccgcgag gtgcaggtag
    17701 gtgaagagct gctgaccttc cttgaagcgg tcgtactcct ccgggatggg ctccttgacc
    17761 ttcacgatca agtccgccgc ctcgaagacc tcttcagcgc tctggaccat ccgcgccccg
    17821 gcatcggcgt actccccgtt cgtaaagccc gctcccgcgc cggcccccga ctgcacaacg
    17881 acctcgtggc cgtgatgcac cagctccgcg accccgtccg gctgcatcga aacgcggcct
    17941 tccttgtcct tgatctcctt cggaacccct accacccgcg ccattgtccg tcctttcccc
    18001 aagactgccc gataccctga ttatcttata acaggcctac agtaaacatg caatgatact
    18061 gtcatatggg ttgcgaagga gtatgaaata gaggtagccg gggttttagc cgctctcatc
    18121 gtcgagggag tacgcggggg cgttgaagtc ccactttata gagatcagcc gcaggatgaa
    18181 gatgagtgcc gcggccgcgg ggccggagat cgcggcggag acgccgaggg cggtcagcag
    18241 gacgtatgcg ccggctccgg cgagcgaggc ggtggcgtag atgtcgcggc gcaggaccat
    18301 cggtatctcg gcgcacagca cgtcgcgcag cagtccccct gccgcgccgg tgatggcgcc
    18361 cattatgacg acgatgaggc tcgggactcc ggccctctcg gcgatctggg ctccggcgac
    18421 agtaaagagc gccagcccaa aggcgtccgc gacgagcagt gagaggcggg gcggccgctg
    18481 gaagcgcgtg tagacgatgg ttgccgctgc cgccaagagg atgacgacaa ggtagttggg
    18541 gtcttcgatc cagaataccg ggtgacggtc gaggaggacg tcccggatcg taccgccgcc
    18601 gatggccgtt acgagggcga cgactatcac cccgaagacg tccatgctct tccggccggc
    18661 gctcagggcg ccgctgaccg cgaagaccgc gaccccgaag aagtccagcg cgtgcagtac
    18721 gctcgccggg ctcacccgct ctctcccgcg ccggagatca cgaatgagtc cgcgcccgca
    18781 gaagaggcac ttgctttact acgcgaaaca ttacgataat agttcaagtg tactaggata
    18841 gatcaagtcg cagagctacg cgcgaagcgg cgcgggtgtt ttggatggga ggcggattgg
    18901 cgactaacgg cgggacggtt gggttctcca taggagaggt ctcgcaggcg atagggctcg
    18961 cgccgcagac cctgcggctg tgggagcggg agcgattgat ccaccctcag aggaccggac
    19021 gaggatacag ggtctacagc gaaacagacg tggagcggct gcgaaagata aagcacctca
    19081 gaaagacgga gggcctgaac ttcgccgcga tccgcaagca gatcggcacc gccgagacgc
    19141 gaaccgcccc tgacacgaag cgcggcgcgc gcaactccgc cactcccggc gaacaactcc
    19201 gcaagctccg catccgggcg cacaagacgc taaaggaggt ctcagaggcg acgggtctct
    19261 ccatctcgtt catctccgcc ctggaacgcg ggggttccgg cgcgtcggtg gcctcgctcc
    19321 ggctgctggc cggggcatac ggggtgagca tgcgcgaggt cttcggagcg gacctggagc
    19381 agaactcgcc gctggtccgc agcgacgagc ggcccgtgat gcagtgggac aacggcgtgc
    19441 gattcgagga gatggcctcc ggcgagaagg tgatggaccc atcatacata aaggtcccgc
    19501 ccggcggcgg cagcgagggc ttctactccc acaacggaga ggagttcatc tacataatct
    19561 ccggcacgct gttcgtggag ctgaaggacc agggctcata ccacctcacc cccggcgaca
    19621 ctctctactt cccatccacc accccgcacc gctggtgggc cgaggacgag cccgtcgagg
    19681 cgatctacgt gaacacccct ccgactttct agccgttctt acgtgagcat agatccgcac
    19741 ctagacgcgc tactctcgat gcccgtcctg tcggggccgg tggtctctcc ggacggccgc
    19801 tgggtcgcgt ggaactggtc ccggctcggt ccggcgacgg acgtcttcgc cgccccgacc
    19861 gacggctccg ctcccccgct ccgcatgacc gggagccacg acgatacgca ggtggtctcc
    19921 tggacgccgg acagccgggc ggtgctcgcg gcgcaggacc acgacggcga cgagcgggcg
    19981 cggctcttcc gggtggcgct ggagcatccg ggtgtaatgg agccgctcac ggaagagtca
    20041 cccggctact acgtccgggg cgggcagacg cacccgaacg ggcgctggct aatctatggg
    20101 gcgaacttcg acgtggagac cggaaaggag atagagccta cctgggtcct ccgccacgac
    20161 ctcaagaccg gcgtgcgcca tacgctggcc cggccggaga ggagcgcctt cgccgagccg
    20221 caactaagca actccggcga gcacgtactc taccaccgga gcgacatcca ccccgagggc
    20281 cggcaggtct ggctcgtgga catcgagggc cacgcggacc gcgagatcct gaacttcggt
    20341 tccaggacga aggtatccgc ctcgtggttc ccggacgggc ggcggatatt gttcctggtc
    20401 gaggcggaga cgcaccggcg gatcggcgtc tgggacatgg acggcgagtc cacgcgctgg
    20461 ctcctggacg acccgcagcg cggcatcgaa tacgctttcg tcccaccgaa cggcggaccc
    20521 gtcgtcgcgg tcgagatgcg ggaggcgagg gtccggtcct ccctgctaaa tgccgagagc
    20581 ggcgaggagg cgcggatgcc ggacctccct ggcaacctga taccactcgc tccggcgaat
    20641 gatggagcct ggatctccct ccactacaac tcccgaaacc cggtggacct ggttcgctac
    20701 tcattgaaac ccggtgagaa cccgagcagc ctcacccgcc tctgggagcg aacatctctg
    20761 gatcctaaga gcctggctcc cgccgaagac ttccgctggc gctcggtcga tggcctggag
    20821 atacagggat ggctgtaccg cgccacgggc cggagcatcg ggacagtcgt gctcgtccac
    20881 ggcggtccca cgagtatcag cgaggaccgg ttcaatgccc aggtccagta cctcgtcggc
    20941 cgcggcttcg acgtgctgtc tcccaactac cggggcagca tcgggttcgg actggagttc
    21001 caggagaaga taaaagagga cggctgggga ggtcgggagc aggaggacat acggaccggc
    21061 atcgaggccc tgatctccgc cggaatagcg gagccgtata gggtcggcgt tacgggcacg
    21121 tcctacggcg gatattcggc gtggtgggcg atcactcact acccgcccga gaccaccgcc
    21181 gccgcggccc ccatctgtgg catgaccgac ctcaccgtgg actaccacac cacccgcccg
    21241 gacctccgcc cctacagcga agagatgatg ggcggcactc ccgaagacat ccccgagcgt
    21301 tacacggagc gttcccccat caacttcatc cagaacgtcc gcggcagtct actcatagtc
    21361 cagggtctga aagacccgaa cgtaacgccc gagaacgtac aggtagtcgt cgaagagctg
    21421 gaccgccggc gcatccccta cgagctactc accttcgagg atgagggcca cggcatctcc
    21481 cgcccggata acctgaaagt cctctatccg cggctggcgg atttcttcga ggaggctttc
    21541 ctggaggatc ggcgcagata gctcagtcgt tgcgggggcg cggccgggat acccggcgca
    21601 gcagagtacc attcgagatc cctctcgttc tcggaagcct ggcagagtct ggcctccaac
    21661 caagcgttac gtatcccggc tcagcctgcg tggaccgcgc tccgaacgcc ccgagcgtcc
    21721 cgaacgctcc gagcggcctc ccgaaccggc gaccatcagc gccgagaccg ccgctatcca
    21781 caccagccct gccagagcgc cgagcagggg gaccaggaag tgcaggagcg ccagcagcgc
    21841 gaccacgaag cccgcgagag ccagccacct cggcaagaca cctgtctcta gcgcgagcag
    21901 ggaggtcgca ctcaccagca ccgccgtacc cacctggcag aggtggtaca gccaggcgga
    21961 gagcgtcagc gacgcgagca tgagccgcgt atcttgcccg aaaccctcga agcggagcag
    22021 ggtggccggg tagagtacct ctgccgcgaa ccccgcgctc gcgagcgtcg cgaacatgac
    22081 tcctccggcc agcgcggccg acgagagacc tcccgcttcg ccctcggcgc gccgcagcat
    22141 cccgtgcagt atcccgagga accagacgaa aaagaaaacg tggaagagcg ggaacacgcc
    22201 gttccacaac acgaaggcgc cgatcccctc gtcaaagtat gaggccgctt ccgagaggga
    22261 gccgggtgcg tcagggtacc cgacgatgta ggcgggtatc atgaggatca ccgacaacag
    22321 gccgcagatg ccgcccaacc ttaccaggtt accgcccgcc atctcgacct cccatgctcg
    22381 acaccaaaac catcaaacgc gaagcctagt atgggtcatg tctgcgacaa tcgctactcg
    22441 acccgtaaac gacctccgcc agccctcaga tcctcccagg ccattcaata ccaacctgcc
    22501 ctgtaatgcc tcgctatact tattctgtct tttatcttgt cttgactcta ttacagtaac
    22561 tgtgtatcgt tcgtgcaatg cgttacattg accggcgcag gccggaggag gagacaacat
    22621 ggacgtaatg cggggtctcg gcgagcacgg tgagagcgga tctctggcgg gagtggacaa
    22681 cctcctgctc cacttcacgc cgtttgcgga ggactggtcg aagctgccgg tgatcgtctc
    22741 gggcgacggc tgctacatta cggacgaccg gggcaaccgc tacgtggacg gtctcgcggg
    22801 cctgttcacg acgcaggtcg ggcacgggcg gacggagctc gcggaggcgg cctacaacca
    22861 gatgaaggag ttaggcttct tcccgaactg gagcttccag caccccagaa gcctggagct
    22921 ggcggacaag ctggcgcgga tagcgccggg ggatctctcc tccacgttct tcgtgtcttc
    22981 ggggtcggag gcggtggaga cggtcatcaa gctctcccgg cagtaccacc acgccaacgg
    23041 ggagccgacg cgctacaagg ttatatcccg caagatcgcc taccacggca ccacgatggg
    23101 cgcgctctcg gtgacggggc tgccgagctt caaggccccg ttcgagccgc tcttgcaggg
    23161 cttccaccac gtacccaaca cgcagcagga cccggagggc gcggcggacg ctatagaaga
    23221 ggcgatagag ttcgcctctc ccgagaccgt cgctgcggtg atcctggagc cggtccagaa
    23281 tgctggcggg tgtctggtgc cgccgccgga ttactggaag cgggtgcgtg agatctgtga
    23341 ccgccacgga gttcttctgg tctcggacgc ggtgatctgc gcgttcggga ggctcgggga
    23401 gtggttcgga atagagcgct tcgacgtggt gccggacatg accagcttcg ccaagggcgt
    23461 gacgagcggg tactcgccga tgggcggggt gatggtgagc gagaggatcc agaacgccct
    23521 gcgctctcac gagcccatgt tcatgcacgg ctcgaccttc ggggggcacc cggtatcgag
    23581 cgcggtggcg ctggagaaca taaagatcat cgaacgcgag aagctcctgg agaacgtgcg
    23641 caacctcgaa ggtcacttcg gggacgagct gaggcggatg gccgagggtc atcccatcgt
    23701 gcaggaggtc cgggggatgg ggttcttctg ggcggtggag ctgaagccgg agcgggccga
    23761 tggcacgccg ctcgaaggcg aggagtacca gaagtacttc aagggcgtgc tctcgcggag
    23821 gctggtcgag aacgggctca tctgccggtt cgacgacaag gaggacccgg tcattcagta
    23881 ctccccggcg ctcatcgccg acagggaggt cctctcgaag atcgcggaga tcaccgacga
    23941 cgccctgagc gagctggaac gagagctggg ctaccgggga tagcggagcg aacggatgac
    24001 ccgccgggag cgcgccgacg ccatcgtcgt gggagcgggc ctctccggcc tgacggcggc
    24061 ccgcgagatc gcgagggccg ggctcgacgt gctcgtcctc gaagccagga gccgccccgg
    24121 cggacgcacg caggccgtgg ggattagagg gtgtcacggt ggacctgggc ggcgaatggg
    24181 tggacgaggc gcacgcggag atccgggacc tcgccaccga actgggtatc cccctccacc
    24241 ccttcgagcg caagaaggag aaagcccggt ggcggttgcg aaacagcctc cacgacgaga
    24301 tgccattatc ggagagggac gcgagggcct accggcggat gaacgaggcg ctggtggaga
    24361 tagcttccgg cgcggacctc gaagctccat ggaagggcgc tccggcggaa gatcccagca
    24421 tccatgactg gctgcggcac gaagggatga gcgatgaggg actccacgtc gtcgagacgc
    24481 tcgtctccag ttgtggatcc acggtgccgc tggaccggat gtcgttctac tcctacgccg
    24541 tcaaggtggc gacgcgcggc ggaccgggga tgggcaacga gttccgggtc gagggcggag
    24601 cggggagcat ttcggaggcc ctcgccgcgg acctcgcgga gaaggtacgc tactccacgc
    24661 cggtcatgga ggtgcggcag gactcggatg gcgtcgaggt gcggtacgcg ggcgagggag
    24721 gtcccgcgtc ggcgcgggcg cgacgggcga tactcgccgt cccgtttacg tgttaccggg
    24781 acatccgctt cgatcccgag ccgccgcccg agttccggcg tatgacctct tcctccctat
    24841 atggcgtggt gcgtaagacg ttcttcgtct tcgacgaacc gattgacgct tcgaccttcg
    24901 ccgtcaccga cacgcctctg ggatactgtg ctgcgactca ggcggcgcgg gatggcgagt
    24961 cgagaggcat cgtctccttc tccgccggca ggccgctgct cccggaactg gggcgtccgg
    25021 cggaggagcg caggcggcgg gcggtggcgt tgctccggga aatgtacgat gtccccgagc
    25081 ccgtggcggt cgtcgagaga gtgtggcccg acgagcactg gacgcggggc agctacatga
    25141 tcatgggccc gggagacatg gcggccttcg gtgcgagcat gggcggctct ttcgggaacg
    25201 tatgtctcgc cggggccgag gggattgccg cggccccgag cttcatgaac agcgcggtta
    25261 aggcgggctc gcgcgcgggc cgggaggtgg cggaagcgcc cggagcggga gtcgtgagga
    25321 cggcgtgagg atggcatgag ggcgaggcgg actgctcggc tgggtccctt gtagagttct
    25381 gtgcgtatac ggaatcgaga cgctcaagta acagagtcgc ggcaggttcg taggaaacag
    25441 gaggcaacgg gtggaagata gaaaaacgac acaccggcag acgccgcccg cgggttcgcc
    25501 ggaggccggg cgcggggtgg cggtcaggtt ggcgggcgta accaagaact tcggggagtt
    25561 cgtggcggtg gacgacctca cgctcgacat ctacgagggc gagttcttct cgttgctcgg
    25621 accgagcggc tgcggcaaga ccacgacgct gcgcatgatc gccggcttcg aggagccctc
    25681 acgaggagag atctccgtcg ccggggagca ggtgcagggc gtccccccgt acaggaggcc
    25741 cgtcaatacc gtcttccagt cctacgccat cttccctcac ttaaacgtct tcgacaacgt
    25801 cgccttcggt ctccggcggg ccggcgtaaa gggcgatgag ttacgcaatc gggtcatgga
    25861 agcgtgcgag atggtgcaac tccagggttt cgagaagcgc cagccgaaca tgctctccgg
    25921 cggccagcag cagcgcgtcg ccctggcgcg ggcgctcgtc aaccgtccga aggtgctgct
    25981 cctggatgag ccgctcggcg cactggacct gaagctcagg aaagagctac agctagagtt
    26041 gcgtaccctg caagaggaag tcgggatcac gttcgtctac gtcacccacg accaggagga
    26101 agcgctcacg atgagcgacc ggatcgcggt gatggatggc gggaaggtcc agcaggtcgc
    26161 caaccccgcc accctctacg agttgccgcg caaccgcttc gtcgccggct ttatcggcca
    26221 gacgaacgtg ctctccggcg ccgtcgagac cgtaaacggc gagcgggtaa cgatccgggc
    26281 cggcggcgcg agcggcgcgg agatagaggc ggtcgcccac gaggaggcca gggtggagcc
    26341 gggcggcgaa gcgcacgtgg cggttcggcc ggagaagctc cgcttcgggg actcgggaga
    26401 caacgtgctc tccgcggagg tagtacagat cgtttacctg ggagcgagca cccagtacat
    26461 catgaagctc gcaaacggag ggaggctggt cctgtaccag cagaactctc acgacgcggc
    26521 ggtgggcgcg gtcgtgggcg atgaggtgct ggtgtcctgt gacgccgcca actgcttagt
    26581 gctaggaggc tagacgtgga gcgcaacacg agcattacca ggggcaggtt cctgaaggtc
    26641 atgggcgccg gggctgtggc cggcacgacg ctctcggtgc tggcgtgcca gccgaacacc
    26701 aacgcccaga gcagcggggg cggtggagac ggcggcggag ggaaaccgaa gaagatcaac
    26761 ctctacaact ggtcggacta cgtcgccaag agcacgctac cggggttcga gaagaagacc
    26821 ggcatcaagg tgcagcagga cttctacggc tcgaacgagg agctgctggc gaagctgcag
    26881 gccggaggca cgggctatga cgtgatagtg ccctcggact acatggtctc gatcatgcgc
    26941 aagtccgaag tcatcgaaaa gctggacaag tccaagatcc cgaacttcaa gaacctcgac
    27001 ccggacttca agggcctccc ctacgacccg aacaacgact actccatgac ctaccagtgg
    27061 ggcaccaccg gcatcctcta cgacccggaa gtcgtcgagg aagaagtaac cggctgggac
    27121 gcgatgtggg acgagaagta ctccggcaag atcgggatgc taaacgacgt ccgcgagacg
    27181 ttcggggcct cgctcaagcg tctcggatac tcgctcaact ccaccgacaa gggcgagctg
    27241 gacgaggccc agtccgagct ggagaagcag aagccgctgt tgcgcgggta cttcggctcc
    27301 ccggagggcg gggacctggt cgtcggcggc gacctggcgc tggcgcacgt cttctcgggc
    27361 gacgggtttt ccgcgatagc cgagaacgac aagctcgtct acatcatccc cgaccagggc
    27421 gcgacccgct ggacggacaa catggcgatc cccaagggcg cgcccaaccc ggacgtcggc
    27481 caccagttca tcaactacat cctggacccg aaggtcggcg ctgacctctc caactacacc
    27541 tactacggat gcccgaacaa ggcatctatg ccgatgatcg acaaggcggc caagaacgac
    27601 ccggcgatct acccgccgcc gaaggtcttc gagcggctgg aggtcataaa ggacctgggc
    27661 tcggccacca aggactacga gcgccgcttc accgaagtaa agagcggcta ggatgcgcgg
    27721 ccggaaagga ggctggtccc tggggctggc ggggttgctc gcgccggtga cgttgtggct
    27781 cgggttgttc ttcctggtgc cgctactgtt gatcctggcg tactcattcg ggaccagcgg
    27841 gccttacggc gggatcacgc tcggcttcaa ccccggcaac tacctcaacg tcttcgaccc
    27901 gctgtatctg cagatcgtgg gccgcacctt cgtcatcgcg gcgatcaaca cgctcatctg
    27961 cctgcttctc ggctacccgc tggcgtactt catcgccttc aagggccgca gacggcggac
    28021 gatgctcata ctaatggtca tggtcccgtt ctggactagc ttcctgatcc gggcctacgc
    28081 ctgggtcgtt atcctgaacg gcaacggcat cgccaacaag acgctccagt tcctcggcat
    28141 caccgacggc ccgatcaacc tcatcttcac ccccggagcc gtgacgctcg ggatggccta
    28201 cgcctacctg ccgttcatga tcctgcctct gtacgccgcc ctggagaagt tcgacgtgcg
    28261 cctcaaagaa gccgcccagg acctcggggc gagccggtgg cacacgttct ggcgggtaac
    28321 gttccccctg agcatgcctg gcgtgatcgc gggcagcatc ctcgtcttta tcccgtcggc
    28381 gggggagttc gtgataccgg acctgctcgg cggctcccgc acggtgctga tgggcaacct
    28441 cgtgcagcag cagttcctcc aggcccgcga ctgggcgttc gggagcgccc tggcgatgat
    28501 cctcgcggcg ctcctgctcg tcgccatcat gttctacgtc cgtaaggtcg gcaccgaaaa
    28561 gctggagggg gtgtaggatg gcggctgtca ggggcgggcc ggtgtacaag gggatgggca
    28621 agctacccga ccgggcattg ctcgtgctat cggtgttcat ctacctcttc ctctacgcgc
    28681 ccatcgcaat cgtcatcttc ttctcgttca acgacgccaa gagcacccag aactgggccg
    28741 ggttctcgac ccggtggtac ggggagttga tccgggacca gaccgtcctg gatgccttct
    28801 acaactccat gatcgtcggc gtaacggcga cggcgatagc caccgtgatc gggacgctga
    28861 cggctctcgc gctctcgcgc aacgacttcc ggggcaagac gttcgccgac tcggcgatct
    28921 acgccgcgac cgtgatgccg gagatcgtgg tcggcgtgag cctgctggtc ttcttcgtcg
    28981 ccgcccagat aggcctgggg atcacgacga tcatcatcgc tcacgtcgcg tttaccatct
    29041 cgttcgtcac gatagtggtg cgggcgcgtc tctcggggat ggaccggtcc atcgaggagg
    29101 cggcgcagga cctgggggcc agcccggtcc agacgttcct gcgggtgacg ctgccgctga
    29161 tactgccggg ggtgatggcg ggggcgctcc tggcgttcac gctctcgttc gatgacttcg
    29221 tgatcacgtt cttcgtctcc ggcgtcgggt cgagcacgct gccgctgaag atctactcga
    29281 tgctcaagtt cggcgtcacc ccggtcatca acgccctctc taccatcgtg gtgttcacga
    29341 cgctcgtcat catcctgggc gccgggcgct tcttcgccgg tggcgacgat ctgaagaacg
    29401 ccaggaagga ttcgtaagta gtgatgaaga atggtctcct gccggtcatc aacccggctg
    29461 cgaaggagcg gtgtcatccc tgcggcaaag gcaatgatcc aggaaagagg gtgatgtagc
    29521 ttgacaaacg cgccggaacg gatcgcgccg ggcgtctatc gcgttgacgc tgtaggcctc
    29581 tcgaacgtca taaacgtgct gctgctggaa gacagcggcg gctggacgct cgtggatacc
    29641 ggggtcggga gcagtgccgc tcgtatacag gatgctctgt ccgcgctcgg ctcgggaccg
    29701 gaagacctca agagcatctt cctcacacac cagcacaacg atcacgtcgg cggactacac
    29761 gcaatgctgg agtgggcccc gaacgcgaag gttggagctt cggagcacga agcggatgta
    29821 atatcgggcc gacgcgggtt cgacccgcag agcagcccgg tcctccgcta cctgtcccgc
    29881 aacgcgaagc cgccaggagt gaccgttcag aaggtggtac gcgatgggga tctcgtctcg
    29941 gggttccgcg tgatcccaac gccgggccac actcgtggtc acgtttcact cctgcgggac
    30001 gaggacggtc tcctctttac cgccgacgct ttcgggtgcc tgccgcgcaa gctcagggtc
    30061 ggtgtccgaa aagctttctg cacagacccc gagcaggcga ggcgctcggc cgggaagctg
    30121 ctcgacgaag acttcgcaac ggtggttttc gcccacggca agcccctgcg gaccggagcg
    30181 aaggcgcggc tgcgcgaggt cgtggcgaag tgccggtact agaaagatcg ttggcgcaag
    30241 ggtgaacgcg ccgacagcgg ccgggggacg ctacgatgcc atcgtcatcg gctcggggca
    30301 caacgggatc atcgcggcgg cctacctagc gaaggcgggc aagagcgtcc tggtgctgga
    30361 gcggcgcggg gtcatcggcg gcgcgacggt tacggaggag ccctggcccg gctaccggct
    30421 ctcgacctgc tcctacgtgt gcagcctgct gttgcccgag gtgatccggg acctcgacct
    30481 cgtccgacac ggctacgacg tgcgcgcctt cgacccacaa tacttcgtcc ccttcccgga
    30541 cggccggtac ttcatgtctc acctcgacag cgcgaagacg gaggccgaga tccgcaagtt
    30601 ctccacaaag gacgtcgccg cctacggcgc atattggcgg atgtgggacc gaatcatcgc
    30661 ccggatgcgc ccgctgctga tgaagtccgc tcccacgcag gccgacatag agcgcgcctt
    30721 cgacggcccg gagggtgagg aggactggcg gacgctcacg aagaagagca tcgcggaggt
    30781 cctggacgaa tacttcgaga gcgaggaggt gaaggcgcct ctgtgcgtcg gtggtgtgat
    30841 cgggacgaac gccggtccac gaacaccggg caccgcctac gtcaaatatc atcacatcat
    30901 cggcaacgtc ggaggcaacc agggagcgtg gggtttcgtc cggggcggga tggggtcggt
    30961 cgcgcactcg atagcttctt cggcaaggga gcagggagcg gaggtcctga ccgatgccga
    31021 ggtggagagt atcgaagtcg aagacgacag cgctcgcggc gtgacgctac gcgacggccg
    31081 gcgcttcgag gcgggcgtga tcctctcgaa cgccgacccg caccgcacct acctgggcat
    31141 ggtcggcgag aggcatctgc cgcaggatct cgcggaaggc gtgaagcgca ttaacgtgaa
    31201 aggctcggtc gtgaaggtgc tgctcgctct cggagagcta ccggacttca cggcgctgcc
    31261 gggcaagaac gtcgggccgc agcacacggg cggcatcgta atcaacccat cggtagacta
    31321 cctgcaacgg gcgtgggagg actgcgaggg gggacggccg tcggagcgtc cgttcatgga
    31381 aggctacgtc cagagcgcga cggagagcgg actcgcgccg gagggcagac acaccatgag
    31441 cctcttctgc cagtacacgc cgtacacgct ggataagggg acgtgggagg agcgtcggga
    31501 ggaaatcggg gcgaacatcg tcgagacctt cgctgagtac gcgccgaacc taccggacgc
    31561 tatcgagcac atggaggtac tgggaccgcc ggacatcgag gcgaggatcg ggataacggg
    31621 cggcaacatc tttcacgggg agatcctgcc ggaaaagatg ttcgctggcc ggcccgtccc
    31681 cggctactcg gactaccgca ccccgatcaa caacctctac ctgtgcggct ccggagcctg
    31741 gcccggcggc gcggtcttcg gagcgccggg ccgcaactgc gccctcgaag ctctggccga
    31801 tctggacgtg aagacggccc gcgcgcgtca cgtttcatag aaactcgacc aaaaggagat
    31861 accgtggaca cgaagagcaa gctgcagaac ttcatcggcg gagatcgcgt agatcccgcg
    31921 aacggccgtc tctacgaact ggtgaacccg tccaccggcg aggtgttcgc cgaagcgccg
    31981 gcttcgagca aggaggacgt ggaccgggcg ttcgaggcgg ccgagaagtc gttcgagacg
    32041 tggcgggatt cgacgcccag tcagaggcag ctcgcgctgc tcaggctcgc cgatgcgata
    32101 gaagagcggg cggaggattt ggtccgcgcg gagtccgaga acacgggcaa gcccatcggg
    32161 ctcacgatgg aagaggagat cccggcgatg gtggatcaga tccggttctt cgccggagcc
    32221 gcccggtgtc tggagggcaa gtccgccgga gagtacatgt ccgggatgac ttcctttatc
    32281 cggcgcgaac ccgtcggggt ctgcgcgcag gtaacgccct ggaactaccc gatgatgatg
    32341 gcggtctgga agttcgcccc cgccatcgcc gccggtaaca ccgtcgtcct caaaccctcc
    32401 gacaccaccc cggtaacgac cgtcatgctg gccgagatgg cagccgagtt cctccccgaa
    32461 ggggtcttca acgtcgtctg cggcgaccgg gacaccggcc gcgcgctcgt cgagcacccg
    32521 acgccgcaga tggtctctat caccggctcc gtgcgggcgg gtatggaggt ggcgaagagc
    32581 gcagccgcgg acctcaagcg agtccatctg gagcttggcg gcaaggctcc ggtcatcgtc
    32641 ttcgacgacg ccgacatcga ggcggcggcc gaggagatct ccgttgccgg ttacttcaac
    32701 gccggccagg actgcaccgc cgcgacccgt gtactcgccg cccccggcat ccacgacgac
    32761 ttcgtggccg ccctcaccga gcaggctaga ggcaccaaga cgggcgcccc cgacgacgag
    32821 gacgtcctct acggaccctt gaacaacccg aaccaactcc agcacgtctc cggcatggtg
    32881 gagcgcctgc ccgaccacgc cgaggtcgtg accggcggcc agaaggtcaa cggttcgggc
    32941 ttcttctacg agcccacggt tgtttcgggc ttgaggcagg acgatgaggc ttcgcagaac
    33001 gagatcttcg gtcccgtcat caccgtccag gcgttctccg atgaggacga ggcggtcagg
    33061 tgggcgaacg gcgtgaagta cgggctggct tcgagcgtct tcaccaggga ccacggccgg
    33121 gcgatgcggg tcagcaggcg gctggacttc gggtgcgtct ggatcaacac tcacatcccc
    33181 ctcgtcgccg agatgcccca cggcggcttc aagcactccg gccacggcaa ggacctctcc
    33241 atgtacggcc tggaggacta cacccgcatc aagcacgtga tgacgaacct gggttagtca
    33301 gtgggtcagc gtgggcgctc cgatggggcg cccgcgagga tgtatctgct acaggcatct
    33361 gataaatacc tcccccgcgc ccttgctatt gcacaacgcg cgctgttata agtacgggag
    33421 cagaacagcg ccctggcgag ggactcagcc gaaggaggca aggggtatgg tcgagacgtt
    33481 acttttcttc ggtatcggtt cgcttgccgt ggtctgcgcc gcaactcttg tagtctccac
    33541 tctcgccctt cggaccgcgc gccggtatgt ggatctggcc gaggagcgcc tggaggacct
    33601 gcgcgaagga caggaccgac tccttgcgtt gctgagagaa gaacgtcata tcccgaggaa
    33661 agagccggaa caggagagcg aagagcagga tcttgagcgc gtagctcgaa caaagagaga
    33721 gcttctgggg cttcgaacgt ctccccggcg ccaggaggcc gggccggaga tccctccgtc
    33781 tccttccgat gggctggttg aggacacacc agcgccccgc caccttccct gggacgtgga
    33841 actaccgaac acgggaacac gagagggagc aggtacaggg ccatcgggaa cgaaagcccg
    33901 gaaaccaggg gagaacaatc cggaagacaa gagtcagcgt cgcgcggtgt ggcgcccgca
    33961 cccggacgac gacatcgctc tgggaagagc ggcggagcgg acacgttccc agagcgactc
    34021 cccggtcgag atgttccgca ggcactacga caagtacctg gagaactacg aaggttacgt
    34081 gaagctggca gagagaatcc accggatgcg gcgcgaatcc ggagtggctc ccgattcccc
    34141 ggcaggacgg gagtgggaag agaggctgcg ccgggcaaac gacggaatcg acaggacgat
    34201 cgcgaggctc gacattctgg aggagtacaa ccccgaactg gccaccgacg accgcatctc
    34261 ccgccgagcc agcatcgctc acagtcactg gaagctcgat agaggcaagc cggaacacag
    34321 ggattcctga aacctgttcg ctatcgcggc tccgatctag aagcgatcta gacggttcta
    34381 catctccttc ccaagaccgg cctcgcgggc gcggatgatg gcctgggcgc ggtctgcgac
    34441 ctggagcttg gtgaagatgt tcgagacgtg gttgcggacg gtcttctggc taaggtgcag
    34501 ctttttggct atttcggggt tcgtctcgtg gcaggcgatg agctccagta tctcgtgctc
    34561 acgctcggtt agctcgggga aggcgtcttt ggccgcggcg gggccgggac tggcgaagta
    34621 ctgcatcaga cgccgggcga tagctgcccc gaagatagcc tcgccgctca tgacggacct
    34681 tatggcacgc aggatctcgg cttttagagc gccttttagc aggtagccgc gggctcccgc
    34741 cttgagcgcg gcgaagacgg agtcgtcgtc ctcgtacatg cttatgacga ggacgcttat
    34801 gtgggggctg gtgcgcagga tgcggcgggt agcctctata ccgcccacgc ccggcatatt
    34861 gaggtccatc aggatcacat ccggctgcaa cttcgccgcg agctttaccg cttcctcccc
    34921 gtctctggcc tcccccacca cctccacgtc cggggcggag agcagcaggg cgcgcagccc
    34981 atcccggaag tgcggatggt catcggctat gagaactctt gtcaactcca tgctcaaggc
    35041 ttcccgatca cctccggctc tacagcctgc cgcgcccgcg cccctcgcgc aagcggcaag
    35101 acggcccgga cgctggtccc cccggaaggc gcggccccta ccttgcagga accgccgagc
    35161 tcctcggcac gctcgcgcat cgaatgaagc cccacgcccg ccgaacggtc ctcggagata
    35221 ccacgcccat cgtcaacgac ctccacgcgc agcgcctcgg cctccggcgc gatccgtacc
    35281 acgcagttgc gcgctccggc atgtcggatg acgttggtta cggcctcctg gacgatgcgg
    35341 taggcggcga cctccaccgc ggcgggcagg ggcggaagct cctctggggc ctccactctc
    35401 acgcgcaggc cgttgtggtc gccgtgcgcc gcctgagcct ggagcgcgcc gagcaggcca
    35461 agctcgtcga gcgcgggtgg acggagacca tagaccaggc gccgcacgtc ggtgacggct
    35521 tcctgggtct ggctcgtgat ctgctccagc agagcgccgg aacgctccgg ggatgtctcc
    35581 agcaagtccc gcgccgcctc ggccttcatc gtgaggctgg cgagttgcgg tcccagaccg
    35641 tcgtgcaggt cgcgccggag cctgcgccgc tcttcttcac gcgccgttac gagttgctcc
    35701 ctcgacttct gcaggtcttc ggagagtctc agggcatcct cggtcaggcg cgtggcgtgg
    35761 acggcgaggc tgacctgccg ggcgaggtcg tccagcaacc gatggtccgc gggagagaac
    35821 tccgcctcgc cggcgcgccg ccccaggacc agccgcccca ctcgctcgcc gccgtagacg
    35881 agtggcaggc gcagcggagt gtccaccggc tctcccgtgg ccgccgcgct ctcgtaggaa
    35941 ccgttccgtt cgatctcgat ggccgcgtac ggcgccttaa gggcctcggc gaccgtcttg
    36001 actacggtgg acagcacggc gtcgggggcg agcgtggctt ccaggtgttg tccgaggcgc
    36061 gagagagcgg cgtaagggtt gtcgcgctcg ccgtacatca ggcggtttat ggcacgctgc
    36121 aaacggtcgc gcaggggctg gaagagtacg gctaccaggc tggtagccac gagcgatatc
    36181 aagaagctgt cttccgtgtg gagcagcgct ccgaggtagc cgaccacgaa ggcgtagatc
    36241 ccgattacgc aggcggtaag cgtgccatag acgagggcgc ggtttatgat gaggtcggcc
    36301 ttgaacagat ggtaacgctg catcgcaatt ccaaaggcca caggcatcgg cagcgcgagc
    36361 agccagaata ccgggacgcc gaagaggtcg tagagcgccc gtgcggagcc cgactccggc
    36421 aagagcacgt ccggcaagta cactgctcca tagccgatgc acgccccggc gacgatcagg
    36481 atgacccact tggtcatgat gcgctgcctc tggtccgccc ggcggtagcg caccgcctga
    36541 gccatcaaac ccaggcccca tccgagcagc aggaggatca agaacgcggc gaacgacgcc
    36601 tcgaacggat ctatgaggct gaaccacgcg gtggggaata tcccccacgc cagacagtag
    36661 aaaaaccagg cgacggcgct cgcgcgagtc catccgggca cgaaacggcc gtccgggaag
    36721 atcaggaaga acgtgaccgc caggagcacg cccgcggcct gcgtcaggtt ggcgatcacc
    36781 tgcaagacgg atggcagcac tagggcgtca agtgtctggg tcacccatac cgcgtaagct
    36841 acaaagacgg tggaaaagaa cagtgagggc cagtcaggct tgttgcgcca cgctatagca
    36901 atggagatca gcgtcaggga gagcacgaag agtatctcca ggaacaacac ggcgatggcg
    36961 tagtacgtcc cctcagagag gaagctttgc agagagccga ggccgggacc tagctgcgtt
    37021 gatgcccggc gggcggtctc taccagttcc atgtatctgg cgggtatgga cagcacgaac
    37081 agtaccagcg aaatcccagc cagcacgcgg ccccctacac gcgcggcgat gggcgcgcgc
    37141 ggcgtatccg gcgtcgcggc ctcagatccc atgacctcct ccgccagcgt ggcggcgagt
    37201 accgggggtg gaccacccgc cctgctcatc cgcagcccct accataagta aataatgaca
    37261 cgaacatcaa ctcagcgcat ctcccgaaag gtctggcgcg tcagaaggcg ccgggtaccg
    37321 gacggagaac gcgggaccag tcccgccggt cagatccagg acgccagaca gcaacatgag
    37381 cggcggcaaa cgtttcttac tcctctcatc agacggcggg ggccgaggcg accggcgtct
    37441 ctttctcgga gactagcgcg tagccgagcc acgtccaggc gagcgccgta ataatgaagg
    37501 gcgggagcaa gagcaggtag gactggtcga tgccgctcaa gaaaagggcg agctgccagg
    37561 cgggtcccac gcccatcatg accgccgcct ccttcggaaa gatacccgcc ctgtaagtgg
    37621 cgacgcctag caggaccgag cctgtcgcgt agaagaagtg ggagatggcg tgcgtagcga
    37681 aaatgggagc agccaacgcc gcctcatcgc cggataccag cccgatgacc acgatagctg
    37741 cgcttccgat agcccccagc gagagtccga atgccgtcag gtaaaacccc gtcctgcccg
    37801 tgagcccgaa atagctccgc tgccgcaggt acatcccgac cgctcccaac atcaggaaga
    37861 cgtacatcgg ggcgtagaac aggtagataa actcctggcc cccgaagaaa gtccccctgg
    37921 cctgctcggc gaagataccg taaatcaagt agaccatccc gaaggtggcg atgtacaaca
    37981 cgccgccaag catagcggcc ggaccgccgt atcggatcaa tttcgacggc atccacaagc
    38041 tcctttctct taggcgcgac ggcgtttcgt ctccacggcc agaactagcc acgcgtcggc
    38101 ggaaaatcag cgcagttgac atagaccgta agggtcccac tcgcggtcgt gtcggggtta
    38161 tctaagatta cgtgccatcc ccctgggttg acctgtccgg tctcggcgtc gaacctttcg
    38221 tccacccgct gttcgctgaa gaccttcagg taagccccgg ctcttgcgat ggcgtagctg
    38281 ccgctcacgg ccacgtctcc cgtatcgcaa gaggttgtta cgccatccga agagccagca
    38341 ggtatcgtct gcgttacgga tttctcgtag atctcggccg gcaggaattt tgaggagtcc
    38401 tggccgtcga gcttgtcggc gtcggcagcc ttgccggtgc tcgtgaggaa gcccgtggaa
    38461 tctacgccat ccagcttgtc ggagttgagg ttggctactt tggttgcgga gttgaccttc
    38521 atcggcgcct tgccggcgct tacgcgaaga tccagcgccg ggccacccga ggggttgctg
    38581 accagcagcg cggcgccact cgccaccttg cccaccacgc ccgtcaccct ggtggcgacg
    38641 ttggtggcct tgcccaggat aaggctgccg ccgttggctc cgaaggcttc ggaggccaga
    38701 ccgaatgtca gagcgagcat taccgccacg cccagggtaa gggccgttcc gcgggctatc
    38761 ccgaacgctt tgctgaggat gttcttcatc gtctcttctc tccttgcttg ctgtccgttc
    38821 ccacgaccag ccctcgcgtc atctgatggt gaagtaccag gacttcgcgc cgacattccc
    38881 ggccgcatca gtggccacta tcttcaccgt cttcttgccc ttcgcgagcc tcggcgagtt
    38941 gtagacgagc gaccccgtcg atgccgcgta gctgtacttc gtggaggaga tgagcacgcc
    39001 gttaacgtag agcttgatgt tggccttgcc aagcggcctg ttgtctttga cgatggcctt
    39061 gatggtcggc gtcgtgtcgc ggatgatcga gccgagcctg ggggacatgc cactgatcgt
    39121 cggctttatg gtgtcgggcg tggcggcggc gacagcagcc ctggcgtcgg ccatgtcgcc
    39181 ggttacggtc ttaccggcgg tcaacgacac cggcttcccg gtgttcatga tcaggttctt
    39241 cagggctacg ggattgcccg tcagcgccgg gttcaccgat gccgccagcg cggcgactcc
    39301 ggtgacgtgc ggcgtagcca tagaggtgcc gttccaggac tcccacgatg cggggacggc
    39361 gtcgtaaccg ccctgggtcc gggcggaggc gccggcgggt gagatcccat cgtggctgat
    39421 cccgaacggg tccgcgagag cgccgtcgaa ggtgtaggac tctccctcga acgccgtacc
    39481 ctgggccccc gtgaaggtat tcgtgaagat atccgggttc acgctcaggg cgagcgtgcc
    39541 cgtcacgaac acggaggtct cattctgata gagcgcgtcc ctcccggtga tgacgagctt
    39601 gccgccccca ttgaggaagt tggtaagggt cgtgagatcc gccggcgtca gggccgggat
    39661 gatgttgaag tcggcgtcga aaccagagta gaaggtctcg ccggtcgccc acacgacggt
    39721 cttgcccgag agctgcgcca gcgaggggcc gtccccgtcg ccgaccggga tcgtctgcgg
    39781 ggcgatgccc gtggcagttt gtatcgccgc cgcgatggtc ggccctacgt ccgcgtttga
    39841 gtcgtgactc cggtcgtcgt ccaccaggat cacctgctgg ctgccacgac cgatggcctg
    39901 aaacaccttc gtcatgaacg acgcgcgccc ggccgcggta cctatctctt cgtcaccgaa
    39961 tccggccgtg atcgccttgc ctgacgtccc cacggacgac agggccgcgg cgggagtccc
    40021 cggactgccg gggaccgagc tgaggatgtc cacgcccggc gcggagatgt ccaccgaagt
    40081 cgccccgtag ttgctgaagc cgccaagacc gcccctgtta ttgacagccg ccaccgacag
    40141 gatgttcgaa ctgttgtacg aggccgggta catcgggacg gtgtcgttgt tgtcgcctac
    40201 cccgtccgcg ccgccgttgc ccgccgcggc gacgaagagg ctgccggagg cttctatggc
    40261 gtccttgagc gcctggctaa aatctcctcc gccccaggag ttgttggaga tcttcgcgcc
    40321 ctcgctcttg gcgtactgga tcgcctctat cgccccggag gtgctgttgc agccgggtcc
    40381 ggtaccggcg ttgctgagga acttcaaggc cattatcttg acgttaggcg cgacgcccgt
    40441 gatgccctgg ttgttaacgg aggcggcgat ggtgccagcg acgtgggtgc cgtggctgtc
    40501 gtcgcccgga tcgtggaccg tcgcgtcctg gtggcagacg tcccagccgt ttacatcgtc
    40561 caccttaccg ttcgcgtcgt cgtccacgcc gttaccgggg atctcgccgg ggctgttcca
    40621 ttgccggccc gccaggtcgg gatgagagaa atccaccccg tcgtctatga cggctacgac
    40681 cagattcggg tcgccctggg ttacggcggc ggcctcgagt ccgtttatgt ccacgttcga
    40741 cacgcctgtg gagccgttga tgacctgccc ggtgttgtgc aggccccaca actcggagaa
    40801 gcgcggctcg tccgcgtagg cggtaggata cagacggtag tccttctcgg cgtattcgac
    40861 atccgggcgg ttctcaaggt cgcggatggc ctgctcgacc ggctgtccgc tcacctcgtt
    40921 tacctcggcg tctataaggt cgaggttttc caccttctcc agcccctcat cccgccgggc
    40981 atcggcctgg gctgccggac ggacgtcttt cttgaacttc acgatgatct ggccgggtat
    41041 cgcctcccta tccggccggg ccttgaaggt cggatggtct cccccggcgg tagctccggc
    41101 cggacgcgcc tctcgcgccg cggcgtccgg caacgtcgtt accagcaccg aaaaggccag
    41161 catgacggcc acgccgaggg cgagggtcgt accccccgcc actctttgcg tcccggatag
    41221 gatggtcatc gtctgctcgt tcctttcacg tcctgatgtt ggttggtcgc gtggccgcta
    41281 tctctcacgc agggctcatg ggcaggcccg gatcgaggct tcgcgggtgt ccgtgacgca
    41341 cctgtcggtg ccggtcccgc cgttgagagt atcgttgccg ttgacgccgt cgcgggagtt
    41401 gagggtatcg ttgccgctgt ccccgagatg tctgtcggat ccggcccctc ccacgaccgt
    41461 atcgttgccg aggccgcccg agagcgtgtc gttaccgaga acgccctgaa tgctgtccgc
    41521 gccaccaaca ccagagatga ggttcgcgag gctcgacccg atgaggctgt cgctgaagtt
    41581 cgagccggtg aggttctcta tcccgaggaa gacgtcggac ccctcgcccg tggcgaagtt
    41641 ggtcgtgagc gagaccctta ccggcgtggc ggagttcgcg tagctcgccc ggtccagccc
    41701 gctaccgccg ttcactatgt cgttgcccag ggacccgacg agcgtgtcgt taccggcctc
    41761 cccgaggatg atgtcgttac cgccagcgcc gttgatgacg tcgttgccgt cgccgccgca
    41821 gagcacgtcc ttgccggagg tgccggtgat gacgtcgttg ccggtggtgg cgttggtgtt
    41881 ggtgcattgc gggaccggct gccagtcggg gtttatgtca tgggcggtac tggtgtctat
    41941 cgggccctgg cccgtgccgt ctgagttcat cacgtagatg ttgcggtccg tggagagtgg
    42001 cgcaacgccc acgtaggcga tctgggtgcc atcgggcgac caggcgggtt gggtggcgga
    42061 cgtgtccggg ttgttggaga cgttggtctt gttggctcca gcggggtcca tcgtccaaat
    42121 gttcggtacg ccgcctccgg cgaacggatt ggtcggaggt ccgtagccgt ggacgtaggc
    42181 gatcttgcta ccgtccggcg accaggcggg gctgtcgtcg tgaccctggt agcagttcgg
    42241 cgagcatccg gccggcgagt tcggcgtgat actggcctga cccgtgccgt ctgagttcat
    42301 cacgtagacg ttgcggtcgg tgtctccggt gcgggcgctc acgaaggcga tcttggttcc
    42361 gtccggcgac caggcgggct gcgtatcggg gaaggtgttg ccggtgagtt gcttgcgtcc
    42421 ggtgccgtca gcgttgatcg tccagatctc caggtccgtc tgaccatcca ggtcgaagct
    42481 gttggagacg taggctatct tcgttccgtc ggccgaccat gcgggttcgg tctttgcggg
    42541 cgaactggtg gtgacctgcc tgcgcccgct gccatcggcg ttcatcacga agatctggtt
    42601 gcccgacaag aaggcgatcc tgccgccgtc gggcgagtag gcgggatccg agctgttgtt
    42661 cgaggtcgtt atcctggtag ccgtctctcc gggggttatg gcgtagatct cgaccggccc
    42721 atcccggtcg ctctggaagg cgatcttgcc gttgttcccc ggaaacgcgg cctcagccgg
    42781 tttcgccgcg agcagcgcca gtagccccag ggccaacgcc gctgccgttg ccatcatgaa
    42841 tagcttgatc tgtccggtct tcgtcatctg caggttcctt tctctgctcc ggttgccttc
    42901 tatctcccgg tcccggagac gctgatctct ccgggacctg actttcttcc tttacttgat
    42961 ggtgaacgac caggacttcg agcccacgtt gcccgccgga tccgtggcta cgatcttcac
    43021 cgtcttcttg cccttcgtga gcctcggcga gttgtagacg agcgaccccg tcgatgccgt
    43081 gtagctgaac ttcgtagggg agatgagctt tccagcgaca tagagcttga tgttggcctt
    43141 gccaagcggc ctgttgtctt tgacggtggc cttgatggtc ggcgtcgtgt cgcggatgac
    43201 cgagccttgc ctgggggaca tgccgctgat ggtcggcctg atcgcgtcta ctgtccaggt
    43261 gcggaccgca gaggtcgcat ccacgttgcc cgcagcgtcc ttcgcccgca cggagaaggt
    43321 gtgagatccg ttcgagagtc ccgagtagcc cttcggagag gcgcaggccg cgaagtcgcc
    43381 gccgtccagc ttgcactcga agctcgcgcc ggtctcgtcg gaggagaatc cgaagctcgc
    43441 cgtggtgctg ttggtgagtg cgggaggccc ggaggagatc gcggtgtctg gcgcggtggt
    43501 gtcgagctga ctatcgagct gaatatcgtc ctgtatcgcc gccgtcgaga cgttgcccgc
    43561 gttgtcgcgg tactcgacgt ggacggtctt ggtcccgtcg cccgcgctca ggagccacga
    43621 acggctcgtc gcgaagggct cccaggcgga ccacgtgtcg gtgttctcgt tgcggaagcg
    43681 catgtggccc acgccggaac cgggcagcgg gtccgtggcc gagagagcga ggctcaccgt
    43741 cgcgttgttc gtggcggcgg catcgttgtt gaccttgatc gtgccggtcg gcgcgacgtt
    43801 gttgactgag acggtcttgg tgtacgcgtt ggtgccgccg tccttatccg tgacctttcc
    43861 cttcaccgtc aggctcggct ggtcgagcgc ggtgcagctc ttgctcgcgg tggtggacgg
    43921 aacgtagccc aatccgtctc cgcagtcgaa ggtataggag agcgtatccc tgtcggcgct
    43981 cgacggctcg gcgacgctcg tgagcgagat cgtaaagttg ctgccctggt ccgtggaagc
    44041 cggagcttcg aaggtagcgg tcggcgcaac gttgttgacc gagatggtct tggtgtactc
    44101 gctgaagccg ccgtccttgt cgatgatccg ggcgcgtacg gtcttggcac cgttgtcgtc
    44161 gaaggcgcaa tcggtggaag tagcggtgcc actgctggcg taggtagccc cgtcgagcga
    44221 accgccgtta cagtcgaagg cgtagtggaa cccggcggtc gtgtcggtct gcccatcttg
    44281 aggagataca aagttgcctt tggtcaggga gatcgtcgcc gtaccgccct cgttgatcga
    44341 cgccggtgcg tcgagcgccg cgaccgggtt gcggttggtg atagtgacgt tcacggtagc
    44401 ggtgcgggaa gctccgtcac tgtcggtcgc agtcgcgctc accgtacccg tcgccgggcc
    44461 gtcgaagaag ccacaccaga cggtgcttga gctctcctgc cactcggacc cgttggaacc
    44521 gcaaccggtg ttcaaggtcc atgtatcgtt gccggggtcg cttaccgaga ggttatatcc
    44581 ataggagctg tagcccagcg cgaaactgcc cccctccggc acgctcggga agctgttttg
    44641 caggttaaca gtgggggcaa cgttgttgac ggtgagcgag aaggtagtag tcgaagtatc
    44701 cgttccgtcc gcagcggtga tcgtgacggt ctggctctga tcgggaccgt cgttggtatt
    44761 gaaggaccag ttccaggcgc cagtagcatc gtccttcgtc acggtcccga cggaggccga
    44821 gagggtaacg gtagcgttgc cgtccgggtc cgagtaggtg ccggtgttga ccgcggtctg
    44881 accctcgttg accgtgacga tagcgttatc ggcggctact tcgggagggt tgttggcacc
    44941 ggggtcgcag gtgtcgcagg gcagggggac cgccttcgcc ggagccgccg cgagagcgag
    45001 tcccagaacg aaaagtagcg ccgccgccac ggcgaggcgc ctcgcgttct ccaacccggt
    45061 aaagctcttg actaacatga ccgccgcaac ctccgctccc tgtctacgcc ccgcgcgttt
    45121 ctgacctgca ctctaagaga cgcggtgtcc cgccgtcatg aggcagtgtc ccggtttggc
    45181 cgggaagaat gtcccgatct cgtgggcttt gatcgctgcg gccggttact tggagagctt
    45241 cacgccgggc gcgggcgacg cgggtctggc aagatgggta gcggcgcggt tacggagggt
    45301 atctagcgtt ctggcggcga atggagggct gaccttgacg accagcggaa tcgaagaatc
    45361 ggcggcgcgg ctactggagg ctttctacga cctgtcgcgc ggcaggctca aagagcccgt
    45421 gcgcctgaga aagtccgact cgccccgggc ggaaggagcc gcgcagagag ccggtatgga
    45481 ccccacctcc accgagcccg acgtggcggt gaggtatctg ctgaaccagg gttatgtcga
    45541 ggcgaccggc acgaacgccg cctacgccat aaccgtaccc ggcatggacc tcgtgcggca
    45601 gaggcgcggc ctggaggatc ctccgagcga aaggaagaag atgagcgaca agcggcagcg
    45661 gcaactggtg acggccctgg cgataattat agccatggtg ctaacacgac cgatcacccg
    45721 ctttatagac gaggagatcc cggagcgccg gggcatcaag gacgacctgc tggaagcggc
    45781 attgcagggt cttgtccgag cggcggcgat cttcgccgcc tccgccatcg ttcggaagct
    45841 ggcctccagg taagagcggc ctactccttt cccaacccgg cttcgcgggc gcggatgacg
    45901 gcctgcgccc tatccgcgac ctgcagcttg gtgaagatgt tggagacgtg gttacggacg
    45961 gtcttgaggc tgaggtagag catcccggca atgtccgcgt tgccgtgtcc ctgcgctatg
    46021 aggttgagga tctcctcttc gcgctccgtt agctgaggga aggcgcgcgg cgggacggtg
    46081 gactgcgggg cggcgaagaa gtccatgaga cggcgggcga tggcaggccc gaagatggcc
    46141 tgtccgcccg cgacggagcg gatggagcgc agcacttcat cctggctggc gtcctttagc
    46201 aggtagcctc gcgccccggc gcgcatggcg gcgaagacgg aatcttcatc ctcgaacatg
    46261 gtgacgacga ggacgtttat gtgagggctc gcgcctgtga tccggcgggt cgcctctatg
    46321 ccgttgatac cgggttatac agcgccagcg cgccgatggt gttccagaag gtggttccaa
    46381 gaagcacgag cagcagcgcg acgaagagcg ccatccgctc gtcggacctt cgccagaaga
    46441 tgccggcagc cagcgccccg cacgccacgg cgaacccgac ctccagcgcg atccggtaca
    46501 cggcatagaa gcccgcggag agcccggtgt ccgccaggtt ggcgcgcatg actgccgggt
    46561 cgatgctctc ggggaggttg gagagggtga ctatctgctc gtagcgggcg ggcgcagggc
    46621 gtgaacggcc cggcgagcag tagagacacc gcgagccagc ctatccgggc cgcgagaagc
    46681 cggcggccgc gcatcccggc cgaagctggg acggactccc cggcgaggct gcctttagcg
    46741 gtttgcgagt tcattgttgg attctcacct gctcgaacct atcgctaaaa tgaatcgtgc
    46801 cacccgttga ggacaagcgc atcttccgta gatactgccc tccccgaaag agccgatccc
    46861 cggcagtccg cgccgccggg gatgaactcg tcttcctccc ggtgctaatg cacccgcggc
    46921 tgggacgtaa ctcccactcc ttcagaagca gacacgtgtc ctcgctccga atcggacatc
    46981 gtgagcatct tcaggccgat ggcgctgagg ctgatcaggc cgagcacggg gccgatagct
    47041 gcgactgccg gaccgccctc gatgtccgat cactccgatc agggcgaggc caacgcctaa
    47101 atgtccgagg acggctccgc gtccacgaaa cagggggagg agaccgctca aagccggcgc
    47161 gaagagcgcc gaggagagca cgatcagtag gctcgcggcc tgcacgcggc cgggatgctc
    47221 agtcaccgtt tgcgcgagtt cagcgttccc tccacctgga tggacaatca tggccacaag
    47281 caacaccagc ggcgcggcga tcaggcacag tcccgccacc atccggcgaa aattgtccgc
    47341 atccattgtc ctcgacatct ctgcctcctt cttcgagggt gaccagctcg ccctgtactc
    47401 gacactctaa gagacgagcg ttcccgcgcc atcagacgga ttcccgaatc ttccgggaac
    47461 tttcgtcccg gcgtgccggg aacaaatatc tgcgttagcc ggacgaggat cccgccgccc
    47521 gcgttcggcg cgtcgaaagg tagaccgccg atcagtggag ttggcgaacg gccctctcgt
    47581 aagcataccc gtaacggtaa acggcagcct cctcaaagcg ccgcacgatg agttggaacc
    47641 ctaccggcaa gccggacgcg gtagagccgc aaggcacgga gaggctcggg aagccgttca
    47701 ggtcggtcgg tccggtgaga cgggtgagcg ccgagcgaac ccgctcctcg tagccgccta
    47761 tgttggtctc cctctcccca attttcggcg ccggtatcgg gacggtcgtg gtgagcagcg
    47821 cgtccacctc cttcagcgcc cggtcgaact cccgcacgct ctctatccgc cgctgctggg
    47881 cgttcgcgta ctggtaggct ctcagcggct ctccgtccat caggcgctct cgtccctcct
    47941 cgtcgaacct ctccggggcc gtcttcacgc gctcttcgtg gatcgcgtag gcctcggccg
    48001 cgagggtaag ccgctgcgcc ttcagggtgt cccagaggtt cggtatctct acctcccgca
    48061 cttcggctcc aagctcccgg aagacctccg ccgcttcccg cacccgcgcc tctgtttctg
    48121 tctcgatatg ctcgaaatag aagctggatg ggataccgat gacggctccc ctcatgccgc
    48181 catccaggtc ccgcgtgaag tcctcgacgg gccggtccgc ggagtagggg tctcgcgggt
    48241 cgtacccggc caggacgctc agcatgagcg cgttatcctc gacggtgcgg gtcagggggc
    48301 cgacgtggtc tagagtccag gcgagcggga agacgccgtg cttgctcacg cgaccgaagg
    48361 tcggcttcat gccgacgacg ccgcagagcg ccgccgggat gcggatggag ccacccgtat
    48421 cggagccgag ggcgccgtag cagaggtccg ccgccacggc cgcgccggag ccgccggagg
    48481 agccgcccgt catcctggtg aggtcgtgag gattgcgcgt cgggccgaag cgcgagcggt
    48541 cgccggtcgg gccgtaggcg aactcgtggg tgtttgtctt cccgataaga atcgcgcccg
    48601 cctctttcag cttctccgcc accgtggcgc tgtagtccgg gacgtaatcc tcgaagaacg
    48661 tagatcccat agtagtgcgg acgcctttgg tgaagatcag gtccttcagc gcgaccggca
    48721 cgccgtgcaa cggaccgcga taatctccgg ctgtgatctc ccgctccgcc cgcctcgctc
    48781 cctccatcgc ctgctctccc accaccgtga taaaagcgtt ggtctcgtcg gcctcgatgc
    48841 gcccgagcag cgcttccacg acctcgaccg gcgagacgcg cctctcccgc agggcgcgag
    48901 agaggtctga gagggttttc ggaaagtccg tcacggtagc gcatcttact cttctgtcac
    48961 gccgctgccc ggcatgcgag agatcagagc caggttccga aggtatacga aggtatagtg
    49021 ggcagatgtt atcgagggat cccggcgaag cccccaggaa gtttttgcgc ggcgcgaagg
    49081 ggaccttcgc tgccttcgac cggcgggtct ggctgctgct ggcggcgatg ctcgtgttcc
    49141 ggttcgggca gggcctctac tatcctttct ccacgatcta cttccacaac gtcatcggca
    49201 tcccgctctc gctggtgggt gtgggactct cctgcctcgc cgtgtcgagc gtggccgcgg
    49261 ggttcgtcag cggtccactc accgaccgct acggacgcaa gccggtgatg ctcgcctcgc
    49321 tctccgggag cgccgcgact ttcctggcct tcaccctcgt ggacggcttc gccggatacc
    49381 tgacggtctc cgtactcgcg ggcctcttcg gctcttcctc cttcgacgcc gcccgcaacg
    49441 cgatggtcgc ggacgtgacg ccgctggggc tgcgctcccg cgcctacggt ctcgtccgcg
    49501 tgggcgggaa cgtgggctgg ggtctcggtc ctatggtcgc gggcctggtc tcggccgcgg
    49561 cgggcggggc tggttggatc tacccgttcc tctttgccgg caccgcggcg ctcacggcgc
    49621 tcgtgatgct ctcgctggcg gtactggcgc gtgaatctct cccggccgtg gcgatgagga
    49681 gtccgtcttc gggaaggttc tccgggaggc gggtcgcgct ctcggacagt ccgttcctgg
    49741 tcttgctcgg cgtcggggtg ttgatgtact acgtgttcac gcaggactgg caggcgctcc
    49801 cgatctacgc gaagaacttc ctcggtgtcc cggacggcaa gatcgggttc ttcctggggg
    49861 cgaacggcct cgtggtgatc ctgctgcagc tccctatctc ctatcttata gaccgcagct
    49921 cgaaagtcct cgccctactc gctggcgcgg ccctcttcgg cgcttcgtcg gcgacgctcc
    49981 tcctcaccga ctccttcatc ggcatcctcc tcgccttcgc cgggttcttc accctggcgg
    50041 agatggtcct ggaggtcgcc ggaccggcgc tcgccgcgga cctcgcgccc gtcaagctgc
    50101 gcggcaccta cctgggactc ttcggctcct gcttcggcct ctcctacggc ataagcccgc
    50161 tcgtagccgg acctctactc caggccggcc tccctcacgc cgtctggacc atccaactcg
    50221 cggcggtcgc gctggcggcc gccgggttga tcgcgctcgc cacgctgcga agacgctaga
    50281 tagatgagta tagatgagta ccggcggacc ctattccggg acgcgcagca ccaggatcgc
    50341 caggtcgtcg cgggggacgc cctcctggaa ctccaggacg gcgtgtttga tcttccacgc
    50401 gatgtccgcg gcgtaggagc cggcatggga gcgcgcgatg tgacgcaggc gttcctcgcc
    50461 gaagaacccg ccttccggag aacgcgcctc cgttacgcca tcggtataca tgacgagcac
    50521 ctcgcccggc atcagacgga gggaggcatc cacgagtccc gggtcgtcga agacgccgag
    50581 cgcctttccg ggacaaccaa cctcctcgat cgagtggtcc tcacgcacga cgagcggcgg
    50641 cgggtgtccg gcgcgcgata ctcttactcc gattccgggc gcgccgtccc gcggctccag
    50701 gcgaatgcag gctacggtgc agaaccgctc ctcggggagc tggcggagca tggcctcgtt
    50761 cagcgcggcg agaacctcgg agggaaggtc ggtcctcatg gtgacggccc ggatggtgta
    50821 gcgggcgagg gccgtaaccg ccgcagctcc ggctccctta ccacgaacgt ccccgatagt
    50881 gcagatatag ccgtcattgg ggacgtctat cacgtcgtag aagtccccgc cgacctcgtt
    50941 ggcctcgccg acgggcaggt actcggctcc gatctccacg ccggggatct ccggcaggtt
    51001 cgagggtaga aggctccgct ggagagtgcg cgcgatctcg ctgcgctcgc tgtagagccg
    51061 ggagttgtcc gacgcgagag cgcagcggta cgccaggctc tccgcgagcg ccaaatcctc
    51121 ttcgtcatac cgatggaccg ggtccgatga ggccagcgtc atcgcgccga tgatcctgcc
    51181 ccgcgccaaa agcggcacgc tcatgatgga ctggggcgcg agctggcgga atatatccag
    51241 gtgctcgccg gtgaggccgc cgcctctgcg gtacacctcc ggcacctccg cgaccagcac
    51301 ggacttcccg gtgcgcagca cccgcgcggt gctgccggga tcatcctcac cgaagctccg
    51361 gtgctcccgg agccggacca gcagatcctc cttctccggg tcggcgtgag cgcaggcgag
    51421 ctgctctacc tcgccgtcct ccccgatcac gtcaagcaga caccagtccg caagctccgg
    51481 cacgacgagc cgggcgaccc tctggagcgt cccctcgtag tcgagcgagg ccgagagcgt
    51541 gggaccggcc gccgcgagca gggagagacg tcttcgggca gcaacggcct ctgcccgcgc
    51601 cagttgctcc cgcacgagca gcaggtcgcg ctcctcctcg gccctcttgc ggtcgctgat
    51661 gtcctcgatc accgcgataa aatacttcgg ctcgcccgcc gtatcccgga cgagcgagac
    51721 ggtcaggttg atccagacgg aagacccatc tctcctgatg tagcgcttct ccagcgtgta
    51781 cgtctgtatc tcgccgcgca gcatccgctc tacatgggta aggtcttccg ccaggtcctc
    51841 gggatgggtt acgtcctgga aggtccgctc cagcagctcc tctcgcgagt agcccactat
    51901 ctcgcagagc ctccggttca cccggagcca tcgcccgtcg atcccgacct gcgccgcccc
    51961 gacggcggcg tgctcgaagg tcgcccggaa ccgctcctcg ctctctctca ggacctcctc
    52021 gatccgcttg cgcccggtga tgtcgttctg cacgccgacg aagttggtca gcaacccctc
    52081 ctcaccgtac acgggcgaga cgtatagctc gttccagaac ggcgtgccgt ccttgcggta
    52141 gttcttgagc accacccggc actcccgccc ctcacggatc gcctccctca tctcctcaag
    52201 agccggctgg tcccgatcct ccccctgcaa gaagcggcag ttgcgcccgc gcgcctcctc
    52261 ggccgggtag ccggagatcc tctcgaacgc cgggttgacg tagacgatgg ggttgtccgg
    52321 gagcttcggg tccgtgatga cgatgccatt ggaactcgcc gacacggcgc ggtcgagcag
    52381 acgcctcaac ttgtccgaag cgcgccgctc cggagcatcg ccgtcccaaa cctttgaaga
    52441 agaggtctcc aacacaacca ttctacagcg cagcccggtc tccgggccat agtaagtcgt
    52501 atataccgta tataaccgtt aacgaacggg attttcgggt atgctagagt agttagcgca
    52561 agctaggaga ggagaaaaga tggcctacga actgccaccg ctgccctacg actacaacgc
    52621 tctcgaaccg accatcgacg agcagaccat gcacctgcac cacgagaagc accacaacac
    52681 ctacgtcacc aacctgaacg ccgctctcga aaagcacccg gacgccgaca agggctccct
    52741 cgaagagctt ctcgccaacc tggactccgt gccggaggat ataaggacgg ctgtccgcaa
    52801 caacgccggc ggtcactcaa accactccat gttctggcag atcatgggtc ccagcggcca
    52861 gggcggaggc tcgcccagcg gcgacctcgg cgcggccata gactcctcgt tcggctcttt
    52921 cgaccagttc aaggagcagt tcgccgccgc cgccgctccg ggagcgctct tcggctccgg
    52981 ttgggcgtgg ctcatcgccg acccttcggg ttcgctctct atagagacca ccccgaacca
    53041 ggacacgccc cttatggagg gcaagacgcc ggtcctgggc ctcgacgtgt gggagcacgc
    53101 ctactacttg aactaccaga accgccgtcc cgactacatc aacgcgtggt gggatgtcgt
    53161 gaactgggac gaggtggccc gccgctacga cgccgcgagg aacgcctgag caccgcccgg
    53221 tagcaccgcc cggtagatct ctctacagac atcagagtgc agtggcgccc ccggaagaca
    53281 gacctccggg ggcgcttcta ttgcgccgtg tgatgcctga tgctctgcgc agtcgtttcg
    53341 ccggtacact tagggtgggc gttgcggcga gcggaagggg atatctggga tgcgattggt
    53401 gatcatcggc ggcgtggcgg ccggaacgaa ggcggcttca cgggcgcgcc ggatggaccc
    53461 tgacctggag ataaccctct atcaggaaga gccagaggtc tctataagcg agtgcggcct
    53521 gccctactac ctgtctggca tggtcaacgc ccgcgaacgc ctcgtcgccc gaacccccga
    53581 gaagttcgcc gcgaaagaca tagaggttct cacccgccac cgcgtcgaga agatagatcc
    53641 cgagagccgc aagcttaccg tccgcgacct gacgaccgac aatacgttcg aggacactta
    53701 cgaccggctc atcatcgcga ccggagcgcg ggccgtactg ccgcccatct ccggcgccgg
    53761 tctcgacggg gtcttcaagc tccgcttcct cacggacgcg gacgaaatcc tgcgatacct
    53821 ggagcgccac tcccctaaaa aagcggtcat cgtcggcggc ggatacatcg ggctggagct
    53881 ggccgagaac ctgtgcgccc tcggcctgga ggtctctctg atcgagggcg cggatcgcgt
    53941 cgccctcgcc tacggccccg aggtctcggc cagggtcgag gatcatctcc gagagaaggg
    54001 cgtaaagatc tacaccggag agaacgtgga agagtttacg tgcgaaggcg accgcgaggg
    54061 ctgcgtcaaa accgtcaggt tcggagagac ggggatcgac acggacctcg tcgtggttgg
    54121 gatcggcgta aaaccgtccg tcgaactggc ccaagaggcc ggaggcgaga tcggtgagac
    54181 cggcgccatc aaggtggatc ggcatatgaa gactaacctc ccagacgtgt gggccgccgg
    54241 agactgcgtg gagaccacga acctggcgag cggcaagcct gcctgggtcc cgctcggcga
    54301 cacggccaac cagatgggcc gcgtcgccgg caccaacgcc gttaccggcc aggacaccct
    54361 ggagttcccc ggcgtcctcg ggaccggcat ctttaaggtc ttcgatctcg gtgtcgccaa
    54421 gaccggcctc tccgagaagg aagccgcgga cgccggcttc gataccgtga gcgccaccgt
    54481 cgaagcgacc gaccgcgccg gctactaccc gggcgtcaaa aaggtcttcc taaacctcat
    54541 cgctgaccgg gcgacgggcc gcatcctcgg cgcggagtcc gccggctcgg gcgcggataa
    54601 gctcgtggac atctgcgcga cagccatctg gggcaggctc tcttacccgg atctcgtcaa
    54661 tatagacctg gcctactcac ccccctacgg cccagctctc agccccgtca tccaggccgc
    54721 caccatactc tccggcaagt tcgagcgcgc cagagaagag gtcagggcct cagagtagca
    54781 gcagctagaa cctctcgacg accgtgcggg cgagcatgtg cagcggcagg tagtcggggc
    54841 cgccggcctt tgagtcggtg ccgctcatgc cgaaaccacc gaagggctgc acgcccacga
    54901 gcgcgccggt tatgccgcgg ttgaagtaga ggttcccggc ccggaactcc tggcgggcgc
    54961 gttcgaggtt gtcgcggttt ttggagtaga cgccgccggt gaggccgtag gggttgtcgt
    55021 tggcgatgcg cagggcgtcc tcgaagtcgc gggcgccgat aacggagagg acgggaccga
    55081 agatctcctc ctgcgctatc ctcgccttcg ggtccacctc gaagactgtc ggggtgacga
    55141 aatatccctt gtccggatct ccggggtcct cgccgagcac ccgctcgccc tcgctcttgc
    55201 ccacgtctat gtagctcgac accttctcga actgcggctc gctgattacc ggccccatgt
    55261 tcacgtccgg gctctcgggg acgccgatct tgagcgctcc ggccttctcg acaactttcc
    55321 tcaggacctc atcgtagatg tcgcggtgca ggatggcccg gctcgcggcg ctgcacttct
    55381 ggccgccgta gccgtaggcg ctcttcacta tgtcgttggc ggcggcttcc aggtcggcgg
    55441 agtcgtcaat gatcatggcg tccttgccgc ccatctccgc gattacgcgc ttgacccacc
    55501 gctggttctc tatctgcttc gccgccagct cgtttatcct gaggcccgtc ttcatcgagc
    55561 ccgtgaacga aatgaagcgc gtgcgcgcgt cggagaccag gtaatccccg atctccgagc
    55621 cgtaacccgg caggaagttc acgacgccct ccgggacgcc cgcctcctcg tagacctcca
    55681 tcagcttcgc cccgacgacg gaggtgaact cggagggctt catgatgacg gtgttgcccg
    55741 tcaccagggc cgcgctggac atgccggtca ggatggcggt cgggaagttc cacggcgcga
    55801 tgattacgcc gacgcccatc ggctggtaga agtagcggct ctcctcgccg gggatggcgt
    55861 agatctccac gccgtctttg agccgcagca tctcgcgggc gtagtattcg aggaaatcta
    55921 ttgcctccgc gacctgcgcg tcggcctcgt tccagggctt gccgccctcg tagacctccc
    55981 aggccaaaaa ctcgaacttg cgccgtttca tgatcgccga cgccctgagc atgatgcgcg
    56041 ctcgcgcctc cggcgcagtc cggctccagt actcgaaagc cccggtcgcc atatctagcg
    56101 ccatatccgc ctcgcgctcg gtcgccgccg cgagcttgcc gacgacctgc gacgggttgg
    56161 ccgggttgac cgaagaaatt tcgccgtccg tctccagccg cttgccgccg atgacgagcg
    56221 ggtagctttt ccctagctgg ccttctacgt ccttcaaggc ggcctgcata gccgaaacgt
    56281 tcttctcgtc cgaccagtcc aggtacgtct cgttcttgta tggtatgagt cccatgaagc
    56341 agctcctatc ttcctatctc ttgatggcgc cggtgatgct cccgatcacg tctttggcaa
    56401 cgtatccggc gatcttcggg ttctccttga ggcggcgggt cgaatattcg taccagtcct
    56461 cgccgaacgg cacgtagatc ctgagcttgt gccccgcgtt cacgagtatg ctctgcaact
    56521 cctcgtccac tccgagaagc atctggaact cgtagcggtc cttcgggacc tctagctggt
    56581 ggatgagccg cagggcgtgc cagatcagga actcatcatg cgtggctatg ccgacgtaac
    56641 atcctccctt gaaaagctcg tcgagcagga agatgtagtt ctgccgcacg gtggcgaagt
    56701 ccttgtacgc taccttcctg ggctcgtcgt agatgccctt gcacagccgc accgatatgc
    56761 ccgactctat gaggggctgc acgtcctcga tgctgcggcg catgtacgcc tggatgacgg
    56821 cgccggtgtt cttgtggcgt tcgtgcatgg tatgtaccat gtcgagggtc ctggaggtgt
    56881 agggcgagtc ctccatgtcc acccgcacga agcggccgcg ggccccggcg tactcgacga
    56941 tctcctccag gttggcccgg catagctctt cgttgatcgt caggccgaag gcggtgggct
    57001 tgacggagat gccggagtcc aggccgtact cctccacggc gtctatgacc ttcttgtagt
    57061 cgcgaagcgt ggcggcggcg tcggccttgc tctcggtgct ctcgccgagc aggtctatcg
    57121 tggcgacgca gccgcctttg ttgaggtctc ggaccgtgct cacggcttca tccagcgtat
    57181 cgcccgcgat gtagcggctg gagatccggc gcacgaccgg ccggggaatt accggtacgg
    57241 accctgctat ggccttgtct ataaatccca cggattatcg cctccagatc gaggtgtgac
    57301 tctcctgcaa ttttactccc ggttccaaga agcgcccggt gtaattaacc gattccagac
    57361 acgcggagca gagaggtttc cctgctccac acgcaagttg gtctagcgtt cgccccggtc
    57421 cgaaggggaa gtagcgtcca cggtctgttg ctggccgccg ccgaagcgcc gccgtatcag
    57481 gaagtaagcc gcgaccacta tgccggcgct taacaggctc agggcgagct gagagcgcgt
    57541 gtccgggatg atgcccatcg agattatgat cgcgacgaag accactatcg tgaagatcgt
    57601 caggtacgga tagagccaca tcttcaactg catccgctcc ggagcttcgc gctcgaacct
    57661 cctgcgaagg atcaactccg agagcgccac gagcagatac acgaacagcg ctatcgcgcc
    57721 cgaggcgttg agcaggaact gaaagaccgt ctccggcgag acgtaggccg cgatcacggc
    57781 caaaaaccca acggatgtgg agatcaggat ggccttcgcc ggcacgccgc gccgggtgac
    57841 gtcgaggaag aacttcggcg cgtcgcctct tctggagagc gagttgagca tccgggaggc
    57901 cgtgtagagc ccggagttca ggcacgagag cacggccgtc aacacgacga gcgccatgat
    57961 ggtgctggct ccggggatgc cgatgtggtc caggacggcg gagtacggtc cgcggccgag
    58021 ggccttgttg gcgttccacg ggagaatagt cacgaccagg aagatggagc cgacgtagaa
    58081 gatcatgacg cggtagatga ccgtgttgac cgcccgcgcc acacccctgc cgggctctgc
    58141 cgactcggcc gccgcgatgg tgacgatctc ggcgccgacg aacgcgaaga tcacggtgac
    58201 gatgccgttg aacgccgcaa acggcccgtt ggggaagaag cctccgtgag aggtgaggtt
    58261 ggagaagtcc agcgaggctc ccggccatag cccgagaacg aagatggtcc cgagcgcgag
    58321 gaacgtgatg atggcgacga ccttgatcga ggcgaaccag aactcgaact ctccgaacgc
    58381 gccgaccgag tagaggttgg tggcggtcag gagcgatacg agtatgagac agaacagcca
    58441 caacggtaca ccgggtataa aggtctccaa aatacccgcc ccggccacgg cctcgaccgc
    58501 cactacgacc acccagaaga accagtacaa ccacccgatg gtgaacccgg cccagccccc
    58561 tagagccatc ctagcgtact cggcgaaaga gccgaccaca ggccgggtta cggccatctc
    58621 tccgagcatc cgcatgacca gcagcaggat aatgccggcc atcaggtacg agaggacagc
    58681 cgccggaccg acggcgccta tgaccgtacc gctcccgacg aagagacccg ccccgatgac
    58741 cccgccgagc gagatcatgg tcatgtggcg catctttagt tgtttcttga ggtcctccgc
    58801 ctgcgccccc tgttgggtag tacccgtagc ctcagccaaa acctctcctt cccccgaaag
    58861 tccggcgctc ccgtacagcg tcccgtagct cccgcacggc gtcgcgcaat ttgaggatca
    58921 cggcgagtaa cgaagaagcc ctgtctccgg caacgcgctc cgccgccgtc cacggcggac
    58981 gcacccgccc cggccgcgcg cccttgtggc tcgaacaccg gaggctaaca ggaagtctca
    59041 gatctacccc tactttgatc cactccttac tttccaggcc ggcagcttat tgtatattgg
    59101 atgcaaagtc aatagccggg gtggctgcgc ggatcaggcg tggtccttgt ggatttcttt
    59161 tgcgtctggt cctgagttct cttttatgta ggtaaagagc agctcgctcg tctcctcgat
    59221 gtggcgggta aggagattca agacggcgtc ggtgtccttc ttgcggcaca gttcgagcag
    59281 ctcgtagtgt tcgcgctggg gtttctcgta ctccgagagg gcgaggtgca tccggatgta
    59341 gcgctcgaag gcgtcgtgct gcgctttgat cagggccagg agccgggggc gttcggcggg
    59401 agcgaagagc gtcgcgtgga agcgccagtt caactcgctc cagtaggtga tcaggtcctc
    59461 ctcgcggtct attcggcgca ggatctctgc ggcgcgttcg aggtcgtcat cttccagacg
    59521 ggggagcgcc ttgctcatcg ccatcgtctc cagggcgacc cttatctccg tgatctcctc
    59581 tacctcgtga tacgaaagct cggagaccac cgctccccgg tgcgggatga acgtgaccat
    59641 cccctcgcct tcgagctgcc gcagcgcctc acggacgggg ctccggctca cgccgaagct
    59701 ctcggcgatc tcctcctggc gcagcggctg tccgcccgcc agcacgccgc tcaggattgc
    59761 gtcccgcaac ccgctcgcca ccatgctctg ggtcgtgcga aacttcccgg acgccctggc
    59821 tgctatctca cccaactcca taaccgaaag tatactggat acgatatcca ccacgaacgc
    59881 cgatggtagg atgggaccgg aacaactaag ggactccaag ccaggaggcg ctcgccatgc
    59941 aaaagaccga catccaacgc ggacccgcat ccaaaacccg caccgtcgcc tctctcatag
    60001 gaggacggac gctcgaggac gcgccgggcg gacgcatgac ctctataaat ccggcccata
    60061 cggacgagag agtcgccgag gtattgctgg gcgacgcggg gacgttcgtg gcggcttgcc
    60121 ggatggcgcg tgaggcgcag cgagagtggg cggcggtgcc cgctcccata agggcgcagg
    60181 tcataaagaa gatcgggcgt ctggtcgagt cgaacaagga ggccctggcg aggctcgtta
    60241 cccgtgagat cgggaagcca tacaccgagg ctttaggaga ggtgcaggag atcatagaca
    60301 cctgcgactt cttcaccggc gagggacgga ggctctacgg ccagaccgtg ccgagtgaga
    60361 tgcccgacaa gcaactcttc actttccggg tgccggtagg ggtcgcggcc attatcacgg
    60421 ccggcaactt cccggtcgcg gtgccttcct ggtacctgat cccggccctc acctgcggca
    60481 acaccgtggt ctggaagcct gccgagta
//
LOCUS       DASSMB010000196         8191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1059658, whole genome shotgun sequence.
ACCESSION   DASSMB010000196 DASSMB010000000
VERSION     DASSMB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8191
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059658"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="VFJ72_11885"
     CDS             complement(<1..1527)
                     /locus_tag="VFJ72_11885"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7480205.1"
                     /translation="MNRRAVENLGETVATLAPREATLVTLPDLHPDLAKALHGMGASR
                     LYSHQLEAYERVRAGENVVVATATASGKSLCYKIPAFENALESAANRALFLYPTKALA
                     QDQLGKIGAFKLRGVHPATYDGDTPRALRAEIRRKSNILLTNPDMLNVGVLPNHEAWA
                     DFLRNLRIVAVDEAHVLRGVFGSHVAAVLRRLRRVAMLHGGDPRFVLTSATIANPQEL
                     AESLTGLPFTLVDNDGASSGERRVVFRNPPLLDKEKGERRSLLTEGALVFADLVSRGV
                     RTIAFARTRKGAELIYRYAADRLGVDGARRISPYRAGYTARERRDIESRLFQGDLLGV
                     VSTNALELGVDVGALDAVVCCGYPGSVASIWQQWGRAGRGKDPALAVYIAGRDSLDQY
                     LFENPQRVLGRRVEAARVTMENPYILGPHLLAAAHEAPLDAEDEEYFGPAYRPVAKQM
                     MEARALAASGGRLVYVGGDTPARDISLRSASSETVLIADADGELIGTAEASRAPSELH
                     P"
     gene            complement(1571..2170)
                     /gene="recR"
                     /locus_tag="VFJ72_11890"
     CDS             complement(1571..2170)
                     /gene="recR"
                     /locus_tag="VFJ72_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7480206.1"
                     /translation="MATYARPVERLITELSKLPSIGPKSAQRIAFHIVRGRPDDALGL
                     AEALREVKEKIHPCKRCFNLTEAGECDICRDTRRDQSVICAVEDPYDIGSIERTGEYR
                     GLYHVLGGALSPLDGIDPEDLRIAELLERVEREGTKEIVIATNPNTTGEATALFIAQE
                     LENSPVRVTALASGLPVGGDLEYADEVTLGRAFAGRREI"
     gene            complement(2183..2512)
                     /locus_tag="VFJ72_11895"
     CDS             complement(2183..2512)
                     /locus_tag="VFJ72_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006235174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET7480207.1"
                     /translation="MGRRNMNKMMAQVQQMQQQMQEAQEQLAKETVTASAGGGAVKAT
                     MTGGLELVSIEIDPEVVDPEDVEMLQDMVLAAVNEAVNSSQELASKRLGGITGGLGDL
                     GLNIPGM"
     gene            complement(2517..4448)
                     /gene="dnaX"
                     /locus_tag="VFJ72_11900"
     CDS             complement(2517..4448)
                     /gene="dnaX"
                     /locus_tag="VFJ72_11900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET7480208.1"
                     /translation="MRHATLYRKWRPRAFSGIVGQEPVVRTLKRAIETDRVAHAYLFS
                     GPRGTGKTSTAKVLAMGLNCDGSDGPTPEPDGTCESCRAIIGNSSMDVLEMDAASNRG
                     IDEIRELRDKVNLAPASGRMKVYIIDEVHMLTTEAFNALLKMLEEPPEHVVFVLATTE
                     KHKVLPTIISRCQSFDFRRPSVETLTEKLSEIAAAEGIEAEPEALTVIAREGRGSFRD
                     AEGLLDQLSSFAEGGITAAMVRELLGSVGPEALLETTYALHERRAADALRIVDRLSNE
                     GKDLGQFVGELLSHLRNLMLLPHAPEVALAEVGADERAPLEEQANSIPTAGVVRLVEA
                     LGDSLNRVKRGGDPKLELELTFLKLARDYTEPDVGALLRRLEALEKTVENGVATLAAT
                     SEPPDLMPRTERPRDPSPEAAPERRGPEPITESAAAPVDVSDTPPDAAPEGVEAKPEE
                     PRPEQKTRRTDAGQSGDAQRDLASEWAGIMQELKRRRQALTAAVYGEARVESFDGEVL
                     RIFYPPEQSFHVGMAKDRKHIEELGKVLEERVGSKPRVEVRAAGEDAGNVASSDARPS
                     APSESRTPEAAPRAEREELREEPPEEPRASNGAGEGGSRPVSGAAEGAGDDDIIRDQR
                     EVFEMARERFGLFGQDGGS"
     gene            complement(4543..4810)
                     /gene="ffs"
                     /locus_tag="VFJ72_11905"
     ncRNA           complement(4543..4810)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFJ72_11905"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(4969..5130)
                     /gene="tatA"
                     /locus_tag="VFJ72_11910"
     CDS             complement(4969..5130)
                     /gene="tatA"
                     /locus_tag="VFJ72_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7480209.1"
                     /translation="MMIPNIGMPELIIALVLILLLFGARRIPELARGLGSGVRELRNG
                     LSGKESDDE"
     gene            complement(5152..5670)
                     /locus_tag="VFJ72_11915"
     CDS             complement(5152..5670)
                     /locus_tag="VFJ72_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480210.1"
                     /translation="MKIKSEGAALAAKEDVAGADIAVYAAATLSLVAALIHLWVAPEH
                     FLHWWGYGVFFAVVALSQGLFSLFILRWPESALIPLAGIWGNLVVISLYVISRTWGMP
                     YGPGWGAPFNPDVAHVEDPELLGMSATAAEVAIVILLVALLGGARRRWTVNALLSLGV
                     LLWALRLTGILP"
     gene            complement(5719..7569)
                     /locus_tag="VFJ72_11920"
     CDS             complement(5719..7569)
                     /locus_tag="VFJ72_11920"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7480211.1"
                     /translation="MELLSRTITRKEMLKMGLLGGAALMLPLERKARTQLSISNRIPE
                     SQLPEPFQLPFAVPTVAAIKHSDATTDYYTMTMKQAQLQILPKSLFGPTTVYGYDGLT
                     PGPTIHARKGRKVVVRHINQLPEVSFANYKTWTSVHLHGSASLPQYDGYASDITNPGE
                     FKDYHYPNIQPARTLWYHDHGVHHTAENAYMGCAAQYHIHDEQELSLPIPHGEYDASG
                     KFVPSKYDVPLILRDAIFAQNGDFVYDDQGHSSLYGDVILTNGVPWPVMQVERRKYRF
                     RVLDASISRSYRLALSTGDPFTVIATDAGLMPAPQQTDQLRIGQAERYEIVIDFARYR
                     PGQQIVLQNLSNPNNQDFDSTSQIMRFDVLDSTGDPNNNEIPSVLNPGTDEFYNPMPL
                     KESDATGAAHLEVIRKNGLWTVNGQTWEDVIDSDYKLTVANPKLDQVQVWTLKNPSGG
                     WFHPLHIHLIDGQILDRNGKPPFDYEKGPKDVFYLGENETVRVLIRFGPQTGRYMVHC
                     HNLVHEDHDMMVQFNVGDAFANRADDPNDPILAASAKPISEMGPLWEEPGNGGGGGET
                     TTPEVTTPEVTTPETTTPATVKPPKKTRKRHKNRKRAEGSAPKHRRRRKR"
     gene            complement(7803..>8191)
                     /locus_tag="VFJ72_11925"
     CDS             complement(7803..>8191)
                     /locus_tag="VFJ72_11925"
                     /inference="COORDINATES: protein motif:HMM:NF044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480212.1"
                     /translation="PYGFFEAGPEAGKSIFETLGDLDHHLLLFEGLEPDPRLEAAREE
                     IEGLLDRYELSVNVQRIESANQKLHERYGANASSMLLIRPDGHVAFRSAVADVVGLKI
                     YLDRLFTRRGVRERALSIAHQDQTAR"
BASE COUNT         1285 a         2736 c         2622 g         1548 t
ORIGIN      
        1 gggatgcagc tcactcgggg cgcgggaagc ctccgccgta ccgatcaact ccccatcggc
       61 gtccgcgatc aaaaccgtct ccgacgacgc cgaacgcagc gagatgtccc gcgccggggt
      121 gtctccgccc acgtacacca gtcttccgcc gctcgccgcg agcgcgcgag cctccatcat
      181 ctgcttcgcc accggacgat acgccggccc gaagtattcc tcgtcctccg cgtccagtgg
      241 agcctcgtgg gcggcggcga gcaggtgcgg cccgaggatg taggggttct ccatcgtcac
      301 gcgggccgcc tcgacccgcc tgcccagcac cctttgcggg ttctcgaaga ggtactggtc
      361 cagcgagtcg cggccggcga tgtagacggc gagcgccggg tccttgccgc gtccggcgcg
      421 gccccactgc tgccagatgg acgcgacgct gccagggtaa ccgcagcaga ccacggcatc
      481 gagagcgcca acgtccacgc cgagttcgag ggcgttggtg gagacgacgc cgagcaggtc
      541 gccctgaaac aatctactct cgatgtcgcg gcgctcgcgg gccgtgtagc cggcacggta
      601 cggggagatc ctgcgcgctc catccacgcc cagccggtcc gccgcgtagc ggtagatgag
      661 ctccgccccc ttccgcgtcc gggcgaacgc gatggtcctc accccccgcg agacgaggtc
      721 cgcgaagacc agcgccccct cggtcagcag actccgtcgc tcgcccttct ccttgtcgag
      781 cagcggcggg ttcctgaaca ctacgcgccg ctcccccgat gaggcgccgt cgttgtccac
      841 gagcgtgaac ggcagcccgg tgaggctctc ggcaagctcc tgcgggttgg cgatggtggc
      901 gctcgtgagg acgaagcggg ggtcgccgcc gtgcagcatg gctacgcgcc gcagccgccg
      961 caggaccgcc gcgacgtgag agccgaagac gccgcgcagg acgtgcgcct cgtccacggc
     1021 gactatccgc aggttccgca gaaagtccgc ccacgcctcg tggttcggca ggacgccgac
     1081 gttcagcatg tccgggttgg tcaggaggat gttcgacttg cgccggatct ccgcgcgcag
     1141 cgcccgcggc gtgtcgccgt cgtaagtcgc cgggtggacg ccacgcagct tgaaggcccc
     1201 gatcttgccg agctggtcct gcgccagagc tttcgtcggg tacaggaaca gcgcccggtt
     1261 cgccgcgctc tccagggcgt tctcgaaggc cgggatcttg tagcagagcg acttcccgct
     1321 cgccgtggcc gtcgccacca ccacgttctc ccccgccctc acgcgctcgt aggcctctag
     1381 ctggtgcgag tagagcctgg acgcgcccat cccgtgcagc gccttcgcca ggtccgggtg
     1441 caggtccggc aacgttacca gggtggcctc gcgcggagct agggtcgcca cggtctcgcc
     1501 gaggttctcc acggcgcggc ggttcatgcc ggagaggggt ctcttcttgc gggatttcgt
     1561 cctggagatc ctagatctcc cggcgaccgg cgaaggcgcg acctaaagtg acctcgtcgg
     1621 cgtattcgag gtcgccgccg acggggaggc cgctcgccag ggccgtgacg cgcaccgggc
     1681 tgttctcaag ttcctgcgcg atgaacagag cggtcgcctc gccggtggta ttggggttgg
     1741 tggcgatgac gatctccttc gtcccctccc tctcgacccg ctccagaagc tccgctatcc
     1801 gcaggtcctc cggatctatg ccgtccagcg gcgagagcgc gccgcccagg acatggtaga
     1861 gaccccggta ctcgccggtc cgctcgatgg atccgatatc gtaagggtcc tcgaccgcgc
     1921 agatgaccga ctggtcgcgc cgggtgtcgc ggcagatgtc gcactccccg gcctcggtca
     1981 ggttgaagca cctcttgcac gggtggatct tctccttgac ctcccgcagc gcttcggcga
     2041 gaccgagagc gtcgtccggg cggccccgga cgatgtggaa ggctatgcgt tgggcactct
     2101 tcggcccgat ggaggggagc ttggaaagct cggtgatgag ccgctctacc ggccgcgcat
     2161 aggtcgccaa aggtcccctg agctacatcc ccggtatgtt caggcccagg tcgccgaggc
     2221 cgccggtgat gccgccgaga cgcttggagg ccagctcctg cgagctgttc acggcctcgt
     2281 tgaccgcggc caggaccatg tcctgcagca tctccacgtc ctccgggtcg acgacctccg
     2341 ggtcgatctc gatggatacc agctccagcc cgccggtcat cgtcgccttg acagctccgc
     2401 cgcccgcgga ggccgttacg gtctccttcg cgagctgctc ctgagcctcc tgcatctgct
     2461 gctgcatctg ctgcacctgg gccatcatct tgttcatgtt tctacgtccc ataggactaa
     2521 cttcctccat cctggccgaa caatccgaac cgttcccgcg ccatctcgaa gacctcgcgc
     2581 tggtcgcgga ttatatcatc gtcccccgcg ccctccgccg cgccggacac cggacggctc
     2641 ccgccctcac cggcgccgtt ggacgcgcga ggctcctccg ggggttcctc tcgcaactcc
     2701 tcccgctcgg cgcgcggcgc ggcttctggc gtccgagact ccgacggagc ggagggtctg
     2761 gcatcggatg aggctacatt cccggcatcc tctccggccg cccggacctc cacgcgcggt
     2821 ttcgaaccca cgcgctcttc gaggaccttg ccaagctcct ctatatgctt ccggtccttc
     2881 gccatcccga cgtggaagct ctgttcgggg gggtagaaaa tcctgagcac ctccccgtcg
     2941 aagctctcca cgcgagcttc gccatagacc gcggcagtca gagcctgccg ccggcgtttc
     3001 agctcctgca tgatcccggc ccactccgac gcgaggtccc tttgggcgtc accgctctgc
     3061 ccggcatcag tcctccgggt cttctgctcg ggacgaggct cctccggttt cgcttcgaca
     3121 ccttcaggcg ccgcatccgg cggcgtgtcg gacacatcga cgggcgcggc agcggattcc
     3181 gtgatcggct ccgggccgcg cctctcaggc gcggcctccg gtgaagggtc acgcggccgt
     3241 tcggtccgag gcatgagatc gggaggttcc gatgtagctg ccagggtagc tacaccgttc
     3301 tccacggttt tctccagggc ttccaagcga cgcagaagag cgcccacgtc aggctcggta
     3361 tagtcgcggg cgagcttgag gaaggtcaac tccagctcca gcttggggtc gccgccgcgc
     3421 tttacgcggt tcagggagtc gcccagcgcc tccaccagcc gcaccacccc ggcggtcgga
     3481 atgctattcg cctgctcttc gagcggtgcg cgctcgtcag ccccgacttc cgccagggcg
     3541 acctccgggg cgtgtgggag gagcatcagg ttccgcaggt gggagagtag ttcacccacg
     3601 aactggccga ggtccttgcc ctcgttcgag agccggtcca cgatccgcag ggcgtccgcc
     3661 gcccggcgct cgtgcagcgc gtaggtcgtc tccaggaggg cttccggccc cacgctgccg
     3721 agcagctcgc gcaccatcgc cgccgtaatg ccaccctccg cgaaagagga gagctggtcc
     3781 agaagcccct ccgcatcccg gaacgagccc cgcccctcgc gggcgatgac cgtcagcgcc
     3841 tccggctcgg cctcgatgcc ctcggcggcg gcgatctccg agagcttctc cgtcagcgtc
     3901 tcgacgctcg gacggcggaa gtcgaagctc tggcagcggc tgatgatcgt gggcaagacc
     3961 ttgtgcttct cagtggtggc gaggacgaat accacgtgct ccggcggctc ctccagcatc
     4021 ttgaggagcg cgttgaacgc ctcggtggtc agcatgtgga cctcgtcgat gatgtagacc
     4081 ttcatccgcc cggaggcggg cgccaggttc accttgtcgc gcagctcccg gatctcatct
     4141 atcccccggt tggaggccgc gtccatctcc agcacgtcca tcgaggagtt gccaatgatc
     4201 gcgcggcaac tctcgcacgt cccgtccggc tccggcgtcg gcccgtcgct cccatcgcag
     4261 ttcaggccca tcgccagcac cttcgccgta gaggtcttcc ccgtcccccg tggcccggag
     4321 aacaggtagg cgtgagcgac gcgatccgtc tctatcgccc gcttcagggt gcggacgacc
     4381 ggctcctgcc cgacgatgcc ggaaaacgcg cgcggacgcc acttgcgata gagcgtcgcg
     4441 tgcctcatac gtggagaatc tggagaatgc tatggaacct cacgtgtcca gtatagcgga
     4501 cgacgcaggc tctcacgacg aagaaaaaca aaccaaaaca aaccgcgcac cccgccgtcg
     4561 acgcttacgg atctggcgaa cttccggccg ccgactccgg ttcggctacc ccgaaagcac
     4621 atgaagttac ccgcttagtg ctgctacctt ccggtcctga cacggttcac ggggcctcat
     4681 tgcccgggac cggaccatca acgccggccg caccggtttc aaccaaaccc ggttcacgcc
     4741 tcgggcgggg aattaggccc tgctaggcga tcgcaggtac agggcatcgc catctccccg
     4801 cttagcgcgg cggagctagt gtatcaaacg aacccgaaaa cggaagaaga acgcgggttg
     4861 tgaagtttaa tctccgaaga ctggtacggc tggccccgct cgaagacggc cggccgacaa
     4921 aagccgcccc gttaagggcg gctatcggtt ctctaaacgt ttgcccggct actcgtcgtc
     4981 gctctccttg cccgagaggc cgttacgaag ctcccttacg ccgctgccga ggcccctcgc
     5041 cagttccggg atgcgccggg ctccaaacag cagcaggatc aggaccagcg ctatgatcaa
     5101 ctcgggcatt cctatgttgg gtatcatcat tctcctttta gctcgtgttt tttagggaag
     5161 aatccccgtc agcctgagag cccagagcag cacccccagc gagagcaggg catttacggt
     5221 ccaccggcgc cgggcgcctc cgagcagagc gaccaggagg attacgatag ccacctcggc
     5281 cgccgtcgcg ctcatgccga gaagctccgg gtcctcgacg tgggccacgt ccgggttgaa
     5341 gggcgcgccc cagccggggc cgtagggcat cccccaggtg cgggagatca catacagact
     5401 gatgaccacc aggttccccc atatgcctgc cagggggatc agggcgctct ccggccagcg
     5461 caggatgaac aggctaaaga gtccctgaga tagagccacg acggcaaaga agacgccata
     5521 tccccaccag tgcaggaagt gctccggcgc tacccagagg tggatcaggg ccgccaccag
     5581 cgagagcgtt gccgccgcgt agactgctat gtccgctccc gccacatcct ccttagcggc
     5641 aagagcggct ccttcgctct ttatcttcac tatatgctgc tcctctccgg ccttcgtccc
     5701 gcgctaccga ctagcggcct accgcttgcg acgccggcga tgcttgggag cgctgccctc
     5761 tgcgcgcttg cggttcttgt gccgcttcct cgtcttcttc ggcggcttca ccgtcgcggg
     5821 agtggtcgtt tcaggagtcg ttacttcggg cgtggtcacc tcgggggtgg tggtctctcc
     5881 accgccgcca ccgttacccg gttcctccca cagcggtccc atctccgata tcggtttggc
     5941 cgatgcagcg agtatggggt cgttggggtc gtcggcgcga ttggcgaagg cgtccccgac
     6001 gttaaactgg accatcatgt cgtggtcctc gtggacgagg ttgtggcagt gaaccatgta
     6061 gcggcccgtc tgcggaccga acctgatgag tacccgcacc gtctcgttct cgccgaggta
     6121 gaagacgtcc ttgggaccct tctcgtagtc gaacggcggc ttgccgttgc ggtcgaggat
     6181 ctggccgtct atcaggtgga tgtgcagcgg gtggaaccag ccgcccgagg ggttcttcag
     6241 cgtccagacc tgtacctggt cgagcttcgg gttggcgacg gtcagcttgt agtcgctgtc
     6301 tatgacgtct tcccaggtct ggccgttgac cgtccacaga ccgttcttgc ggatgacctc
     6361 aagatgcgcc gcgcccgtgg catcggactc cttgagcggc atcgggttgt agaactcgtc
     6421 ggtgccgggg ttcagtacgg agggaatctc gttgttgttg gggtctccgg tgctgtcgag
     6481 gacgtcgaag cgcatgatct ggctcgtcga gtcgaagtcc tggttgttgg gattgctcag
     6541 gttctggagg actatctgct gtcccggcct gtacctggcg aagtctatga cgatctcgta
     6601 gcgctcggcc tgcccgatcc tgagctgatc cgtctgctgc ggggccggca tcagcccggc
     6661 gtcggtggcg atgacggtga aaggatcgcc cgtggagaga gccagcctgt aggagcggga
     6721 gatcgaggcg tccagaaccc ggaagcgata cttcctgcgc tcgacctgca tcacgggcca
     6781 gggcactccg ttggtcagga tcacatcccc gtagaggctg gagtggccct ggtcgtcgta
     6841 gacgaagtcg ccgttctggg cgaagatggc gtcgcggagg atcaggggca cgtcgtactt
     6901 gctcgggacg aacttgccgc tcgcgtcgta ttcgccgtgc gggatgggca gggagagctc
     6961 ctgctcgtcg tggatatggt actgcgccgc gcagcccata taggcgttct cggcggtgtg
     7021 atggacgccg tggtcgtgat accagagcgt cctggccggc tggatgttcg gatagtgata
     7081 gtccttgaac tcgccggggt tggttatgtc cgaggcgtag ccgtcgtact gcggcagcga
     7141 cgccgacccg tgcagatgaa ccgaggtcca ggtcttgtag ttggcgaagg agacctccgg
     7201 caactggttg atgtgcctca cgacgacctt gcgtccctta cgggcgtgga tggtcggtcc
     7261 gggggtcagg ccgtcgtagc catagacggt ggtcgggccg aaaagggact tgggcaggat
     7321 ctgcaactgg gcctgcttca tcgtcatcgt gtagtagtcg gtggtggcgt cggagtgctt
     7381 gatcgcggcc accgtcggga cggcgaaggg gagctggaac ggctctggga gctggctctc
     7441 tgggatgcgg ttgcttatgg agagttgggt cctggccttc cgttccagcg gcagcatgag
     7501 agcggcgccg cccagcaggc ccatcttcag catctccttg cgggtgatcg tgcggctgag
     7561 cagttccatc ctcgtgctcc cttcctgtct tctcaaatag cgctgctcaa tgggcagatc
     7621 gcttcgtctc gctcgtcacc tgtattcaac tagggcgggg aaatgcgtac atcggtaaaa
     7681 aaggtgaact ttatctagcg taaaagggcg atctttatct agcgtaatcg ggttacttca
     7741 tttggagtat gctactcagc ttctggcctc cggcgcgcga taggtttctc cgcccgtgga
     7801 tgttaccggg ccgtctggtc ctggtgcgct atcgagaggg ctcgttcccg gacacctctt
     7861 ctggtgaaca gccggtcgag atatatcttc aggccgacaa cgtccgcgac tgcgctgcgg
     7921 aaggcgacgt ggccatcggg ccggattagt aacatgctcg aagcattcgc gccgtagcgt
     7981 tcgtgcagct tctggttcgc cgactctatc cgctggacgt tgacggacag ctcataacgg
     8041 tcgagcagac cctcgatctc ctcccgcgcc gcctccagcc tgggatccgg ttcaagcccc
     8101 tcgaagagga gcagatggtg gtccaggtct ccgagcgttt cgaagatgct cttgccggcc
     8161 tccgggccgg cctcgaagaa gccgtaaggg g
//
LOCUS       DASSMB010000197        36288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_168188, whole genome shotgun sequence.
ACCESSION   DASSMB010000197 DASSMB010000000
VERSION     DASSMB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 36288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36288
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168188"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1083
                     /locus_tag="VFJ72_11930"
     CDS             136..1083
                     /locus_tag="VFJ72_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480213.1"
                     /translation="MVLAGLFFAGCGGGEEPASPPPATTTAPAAAPAAPPAGGTTVGS
                     GQAETIRDRPISLNMDQPVPPDFKTAYQRRALIAVEFYKTGQNPFYPQGLGVDSQVNT
                     FVDQLRTQYPTVEFFSYNIDNPGAGGDNVKLQSGQYGTLAAQLGVGLTPFLATLAPAS
                     NGDGYIIKNLFQGYVTQPVISQALFDLSAVQVEDNTSDVDVKIDQLELTERGGGIEYI
                     TVRNTSPDPVNLQGFTLRTLDPETGQVNDQSATVEINDSIEVQPGKSVSVGRSAEVVD
                     ADGKKVAGVFVGGEDLGLAPGDQVALLDAGGAVAATITV"
     gene            1150..2307
                     /locus_tag="VFJ72_11935"
     CDS             1150..2307
                     /locus_tag="VFJ72_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HET7480214.1"
                     /translation="MSDSVYLDNAATTPLDSRVLEAMLPHLRGRRGNASSLHASGAAA
                     REAVEEARESVAAFLGASPEEIIFTSGGTEADNIAVLGLAVARPEKRHAVVSRIEHPA
                     VRGAAARLERDGYEVSWIGVDENGMVDPEQFARSLRPDTAFAAVMWANNEVGTVQPVE
                     ELAAICRERGVPLHVDAVQAAGRIPLNTASVPVSTLAVSGHKLYGPQGVGALYVREGV
                     DLEPTVFGGGQERGLRSGTHDVAGIVGLGAAARLASEELEERASHEAALRDRILEGVR
                     EKAGVRLNGHARERLSNNVHLYVEGVEAEGLVLFCDALGFEIGSGSACASGGHKASPV
                     LLAMGRSEQEAFSAVRITVGKDNTEGEIEAFVEAFAAAVARLRELSPLYAG"
     gene            2314..3810
                     /gene="mnmA"
                     /locus_tag="VFJ72_11940"
     CDS             2314..3810
                     /gene="mnmA"
                     /locus_tag="VFJ72_11940"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET7480215.1"
                     /translation="MAERYRPQVIEHLVRPRHVGDLPEPDGAGESGDSACGDIARFTV
                     RIEENVVREVGYKVYGCAACIAAGSALAEMVAGEDLLRAASVSKEDVQDALGGPLPPG
                     KEHALTLVLDALHKALEDYLNRRAGSMLVDGYGGGRAGEKKTVVAAMSGGVDSAVTAL
                     LLKEAGYEVACVTFRLHDGERGSRSCCSPDTVLFARDTAHRMGLPHFTLNLKELFNRR
                     VMQDFVGTYEAGKTPNPCVACNAHVKFHAAAFLADRLGFDHVATGHYARVGVGPTLAR
                     PVDETKDQTYVLWPMPKELLARTVFPLGEYRKTEVRKIAEDRGLAVAYTPESQDICFI
                     PDGNYRGFIRKKVAAEPGEIVDTSGEVLGRHAGVVDFTVGQRRGIGVSAPTPLYVTEV
                     RPQSKQVVVGRRRDLEVREVRVSGMNWFLDPGEARAVQVRYNSSPVPCEIEDVGDGEW
                     VAYLEEPVMGVAPGQSAVFYTGDGQRVAGGGVVARNRPKAAAAGGQEI"
     gene            3850..4230
                     /locus_tag="VFJ72_11945"
     CDS             3850..4230
                     /locus_tag="VFJ72_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET7480216.1"
                     /translation="MDTFVELMKALLFAGIAVAFFVLAWMFLRLASIFSTTEQTIKEV
                     SEQVVPLLGKTHVTVDNINSQLSQLDTAVGDVAGITGELDQTTTVITRGVRSGVIKAS
                     SATAGLSRGISTFLGGKKTTEGED"
     gene            4234..4569
                     /locus_tag="VFJ72_11950"
     CDS             4234..4569
                     /locus_tag="VFJ72_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480217.1"
                     /translation="MEGWGKILTGAALGVLGTVYATNEEFRKQLPKSARELPVVVRKR
                     FDEAVSAAREASSSRRQEILRDLEVHGGGAHAARGVEPDPAAPETSVPEPPVVTDPDA
                     TQQLSRPTE"
     gene            4607..4885
                     /locus_tag="VFJ72_11955"
     CDS             4607..4885
                     /locus_tag="VFJ72_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480218.1"
                     /translation="MTTETDAEYTYLEIPPSPEMRGCIGLVMAGMAARAKIGVGGLDE
                     AVEILESLHSEIEPTVYRFSMDEDRVVAQIEDPEEGASIWRTVVELVS"
     gene            4855..5658
                     /locus_tag="VFJ72_11960"
     CDS             4855..5658
                     /locus_tag="VFJ72_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HET7480219.1"
                     /translation="MADGGGTRIVNRRYRRPDKARTRRLLIAYHKEGDQRARERVIAE
                     QLPLAEFLARKFAGRGEPVDDLVQVASVGLIKAVDRFDVDRNIEFSTYATPNILGEIK
                     RYFRDKGWAMRVPRGLQELRQSAKEAIQDQTIKTGRSPTVEELAKMLDSDEESVAEAL
                     TLGRAYNTASLDAPVNQEDTDGDTIIDLQADDNFPIEGLEDKILLQSAMHGLKEQQQQ
                     ILKLRFNEGKTQTEIADQIGVSQMHVSRLLRRAIDDLRVRLGEMEKNGN"
     gene            5701..8337
                     /gene="alaS"
                     /locus_tag="VFJ72_11965"
     CDS             5701..8337
                     /gene="alaS"
                     /locus_tag="VFJ72_11965"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7480220.1"
                     /translation="MQSSEIRQKFLNFFTGRDHRLYPSSSLIPHNDPTVLLTTAGMQQ
                     FITYFTGEDRPPSPRATSAQKCFRTQDIEDVGDPTHLTFFEMMGNFSFGDYFKKEAIE
                     WAWEFLTRDLGLAPERLWITIFEGDENAPEDLEAKDFWMKVGVPVERIFGLPKSENWW
                     GPPGDSGPCGPCSEVYFDYGEEYGEGDPLADPKYGPGGDEGDSRFLEIWNLVFNQYEQ
                     RKDGTLTPLAKPGIDTGMGLERTAAVVQGVHTVYDTDLYAPIFEKAREISGAERGSSD
                     DTDRALYIIADHVRSIAFLIADGVRPGNQRREYVLRRIIRRTALQAYARLGMKPEQIA
                     DLVEVVVDSMGDHYEELERAREDIRRIVTSEAARFVEIYDSGMGLLEAEIERVGGGSF
                     PGEVAFTLHDTYGFPVEVTREVLAERGISLDEAGFERAMADQRERAREAMQGYDRVVA
                     AFRDQEIKSRFVGYEREQVETRIVAVEPVPGAEDEVFVVLAENPFYATGGGQVADEGW
                     ISSDNGQLEVVDTIPAGDYQVLRARVEHGDFHAGDSVTASINRVRRQQIEANHTATHI
                     LHWALRAVLGRDVVQAGSYVGPDRLRFDYRYAGKVSDDELRRIQELCLLKITENQPVR
                     YFTTTMDEARNLGAMMLFGEKYGDLVRVVEVDGFSRELCGGTHVRGTAEVGAFKILSN
                     RKHGADLYRIEVITGREALYYLIGAAERAEEVSEALRVELDRLPEAVENLRQSARQAR
                     EMEREQALKQGLQEVGALVESAEAVDGMKVVTGQVVATDVKGLRQISDDVKNRLGGPA
                     AVVLAADLGGKAVLIANLHPQVSEKIEAGEIVREASGVLGGGGGGGPTMAQAGGGDLS
                     AIPDALDKVRVILRGRLAGREG"
     gene            8342..8797
                     /gene="ruvX"
                     /locus_tag="VFJ72_11970"
     CDS             8342..8797
                     /gene="ruvX"
                     /locus_tag="VFJ72_11970"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7480221.1"
                     /translation="MQASSRATGRIAALDLGEVRTGVALSDPSRTIARPVEVVASEKL
                     EEYLRGLLEDEGVEEILVGVPKTLGGEVGFQARRVLATLGHLKTVFAAVTFIEWDERF
                     TTRMSIAGPGGGSTGRSRKRDRTSGTGGRVDHLAAARMLQEYLETRGNL"
     gene            8794..9912
                     /gene="mltG"
                     /locus_tag="VFJ72_11975"
     CDS             8794..9912
                     /gene="mltG"
                     /locus_tag="VFJ72_11975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7480222.1"
                     /translation="MRRYQKGRRRDREDLEARLNALQPSRSKSRRKRRSNAGPAILGI
                     LLVIGVLGVIYLIYTSATSGSASQQADTRAGKVEVVKGDTLSSVADKLDEAGVVSSAT
                     LFKLEARLSGQSTEIKPGEYTFGPDDDTDAIMKKLTTGKDVPTYAITIPEGLTLQQTA
                     ETVAQSKSGISAADFEKAAKKTDYGYAFLKDPAIKSTEGYLFPKKYDFEKGTTAPQIV
                     NRLLEQYLLETQELDFKGAEDRLNLSEYELVTVASLIEKEAASPEEKPIIASVIYNRM
                     RQGMPLQIDATIQYARGKPKEDLSLDDLEIDSPYNTYKNPGLPPGPICSPSRESLEAA
                     IEPKETDYVYYVLKANGQEHYFTNNYNDFLQAKAEAGR"
     gene            10167..13172
                     /locus_tag="VFJ72_11980"
     CDS             10167..13172
                     /locus_tag="VFJ72_11980"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7480223.1"
                     /translation="MLSREGNHPAAGPARELGEYLVAAGLISEGQADAARDRCRAERG
                     NLAKSLVSLGYLSADEVARALAKVQNVEYVDLSKESLDPDLISLIAEDVLLRHGAVPL
                     RAESGRLIVAMADPGDTEARSDLTISAGHPVTPVVASEEEIRRLHRRHFGTRAEPSSA
                     AAEAPPSGDNGSVRETRSSYAASPRERPGARRPAGRTPKIGEILISEGKLSETQLQEA
                     LTIQRNDPRDLGEILISLGLVEAEDLARALARRLKLDFVVISELSESEVDPDVLNLIE
                     ERTLRKYTALPLRFEDGRLVVAMSNPNDLFALEDLRIMTKHQITPVVATFEDISGALS
                     FVFGDEEALDSKLDEGGSSIEAYEMDEPAPEPEFAQAPPTELAEEPPTEEPSPVDESP
                     EPEAGVDQSRASGDGEWPPETPEPAEGAPEAGGASRGAAVERGARRLGGAGTRIGDIL
                     LAEGKVSAGQLEEAQLTARERRQDLGKTLLSLGYVSEEDLARALAARLRLEFVELTER
                     DVDRAAASLVDQRVLRRLGAMPLRVSDGRLVVAMSDPTNIYALEDLRMISGYPVTPVV
                     ALETEIRRVQNKLFAVGEEVTELLQEAAGESVFEDHGEVELGGDAGAEDAPIVRLVGS
                     VLQQAVGEGASDVHIEPRARELTVRYRVDGVLREVMSIPPKLQSGVIARLKILANLNI
                     AERRVPQDGRFSVRLGGQKIDVRAASLPTVFGEKIVLRLLDTSNVEANLEQLGFRTDI
                     FETYEQIFRRPYGAILVTGPTGSGKSTTLYATLSELNSPERNIITVEDPVEFRMEGIN
                     QIQVNAKAGLTFASGLRSILRSDPDVVMIGEIRDFETAKISVEAALTGHLVLATLHTN
                     NAPSAVNRLTDMGVEPFLTSSAVDCVIAQRLARRLCDYCKEPVEIPSDVLADINFPHE
                     RAFTNGLRFHRAVGCDRCGGSGYRGRLGIYEMMVVTPEIRDLILRRVSTGEISRVAEE
                     QGMVPLRDDGLLKAAEGLTTIEEVLRTVV"
     gene            13284..14417
                     /locus_tag="VFJ72_11985"
     CDS             13284..14417
                     /locus_tag="VFJ72_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HET7480224.1"
                     /translation="MMQEAGLNDYLFDAIRQGASDLHITVGVPPSVRVSGKIRHLDYP
                     ALTPNMTRELIYDILSNDQRQRLESDLELDFAYTLPRTARFRVNVYFQRGSMGAAFRT
                     IPSEIRSFSELGLPKAIEDMTDKPRGLVLVTGPTGSGKSTTLASMIDKINETREEHIM
                     SVEDPIEFLHTHKKCIVNQREVNQDTRSFAQALKHVLRQDPDTILVGEMRDLETIHLA
                     VSAAETGHLVFGTLHTQDAPQTVDRIIDVFPSHQQSQIRTQLANALQGIITQTLIPKR
                     DGKGRVAACEILVPTPGVRNLIREGKNHQIYSAMQTGGKFGMQTMDAALVDLVRKGLI
                     SREEAEKRSSNPEELNRLAGGSMGGASNRPAASSSGSYAALRR"
     gene            14429..15640
                     /locus_tag="VFJ72_11990"
     CDS             14429..15640
                     /locus_tag="VFJ72_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7480225.1"
                     /translation="MAVYTYKARSRSGEVLEDQIEANDQVSVASELRQQGLLVIDIKE
                     QGAAQKDLLEPFKKVKLSDVVIFTRQFATMINAGLPIVRSLYVLGEQTDNPKLNEVIV
                     AVRKDVEAGLGLSEALAKHPKVFSRLYIEMVRAGEIGGILDGVLLRVAAQLENDQELR
                     RKVKSAMTYPTAVLVIALLAAAFMLIFIVPIFAKMFADLGGTLPLPTRIAMGASDILT
                     SFYGILVAAALIGGVMFFLRWKNTEQGRKVWGRLILKMPVKIGDIVQKVALARFARTL
                     GTLSAAGVPILQALEITASSSGNWVIENALLRSRDSIRQGIPIYRPLEDEPVFPPMVT
                     RMIAVGEETGNIDGMLTKIAEFYESEVDASVKALTSIIEPLMIVVIGGIVGGIIIAMY
                     LPMFKIFELIE"
     gene            15679..16395
                     /locus_tag="VFJ72_11995"
     CDS             15679..16395
                     /locus_tag="VFJ72_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7480226.1"
                     /translation="MARLVESYREQGDRRALERLFEMHGKLLNHIVRRYAATSGEPYE
                     DLLQVGYVGLLKAANNYATDSTARFSSYAYSMIDGELRHHFRDTGMVRKPRWARSLYA
                     RVSEATARLTGELGRPPLLEEISREVNVAPEGVLELMKIFLDTDVRSLDDSAGEADLS
                     NIRSIGYETFSLPIEDRIQLEQALDTLSELQKKVVYLFFYKDLSQTEIGKMLGLPQRK
                     ISRIIADSTRSLRDRLSSDK"
     gene            16470..17144
                     /gene="rpe"
                     /locus_tag="VFJ72_12000"
     CDS             16470..17144
                     /gene="rpe"
                     /locus_tag="VFJ72_12000"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET7480227.1"
                     /translation="MAFWGELGDRIVGAPSILAADFANLAEEVRRAAEGGAELIHFDA
                     MDNHYVPNLTVGPVVAASLTEAVGLPVDAHLMVDNPDNLIQPFIDAGVVSISVQPEAV
                     THLHRTLTMIRDGGCEAGVALNPTTPPEFIEWALPYLDYVLVMSVNPGFGGQSFIPEM
                     ADKVRRIKEMTDLPVQVDGGITVETAPLMVEAGAQVLVAGSAVYRGDPVTEMRRIIEA
                     GRAAAR"
     gene            17363..18481
                     /gene="ribD"
                     /locus_tag="VFJ72_12005"
     CDS             17363..18481
                     /gene="ribD"
                     /locus_tag="VFJ72_12005"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET7480228.1"
                     /translation="MAVEAFMDRARDLAERGRFTAAPNPLVGVVIARDGRVIGEGWHA
                     RAGLEHAEVAALSANGTDMRGASMYVTLEPCNHHGRTPPCTDSILRAGISRVVIGHLD
                     PDPRMRGRSVELLRGAGVEVEVLDDPAFERQNEQFFHYMNTGLPFVHLKLAATLDGRI
                     AASGGDSKWITGEAARRRAHALRAEAGAVLVGAGTVRADDPSLTARDVPDQPPPLTRI
                     VLDPSLSLDPESQLARSVDEARVLLFADKTVLDGREKRLEALGVEVVGAPRGENGLDL
                     AYVLAEAGDRGLRGVLVEGGGETAGRFLRSRFVDKVTVFYAPKILGADGVPMIGPLGI
                     TSVQDAPHFSVSGVEQLGDDLAVTFYPRREENDVHRID"
     gene            18474..19094
                     /locus_tag="VFJ72_12010"
     CDS             18474..19094
                     /locus_tag="VFJ72_12010"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET7480229.1"
                     /translation="MIETTGWVVSLEPGGMARLVISAGEVAADARVGDSISVNGVCLT
                     VGEMEGDTLTFYAMPETLRRTALGDLKEGATVNLERAMTPQRRFGGHIVQGHVDGVGW
                     VAGVRPEGDAEIWEFGAPESVLRYTVEKGSICVDGISLTVVGVRESSLTVSILPQTKA
                     ATNLSQLSVGDRVNLEADVIGKYVERLLGPWIDSVRETERSFGDAV"
     gene            19084..20286
                     /locus_tag="VFJ72_12015"
     CDS             19084..20286
                     /locus_tag="VFJ72_12015"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET7480230.1"
                     /translation="MPFSPVEDVIEDIKNGKMVIVCDDEDRENEGDLTMAAELVSADD
                     INFMATYGKGLICLPLAGEIVDRLEIPEMVQHNSARLGTAFTTSIEARDGISTGISAA
                     DRAHTCRVAVGDETGPDDLVMPGHIFPLRARPGGVLQRAGQTEAAVDLSRLAGLKPAG
                     VICEIMNDDGTMARVPDLEKFSKEHGIKMVTVAQIIEYRNTYENHVEFAVETQLPTKF
                     GEFRLRAYENDMDELTHLALVMGEPEGKENVLVRVHSACLTGDALHSLRCDCGEQLDA
                     AMARIAAEGEGVLVYMQQEGRGIGLLNKMRAYHLQDKGFDTVEANQELGFPPDLRDYG
                     IGAQILKDLDLHSIKLLTNNLTKVVGLQGFGLEIVERISLEITPNGYNDRYLATKREK
                     LNHVFESH"
     gene            20296..20838
                     /gene="ribE"
                     /locus_tag="VFJ72_12020"
     CDS             20296..20838
                     /gene="ribE"
                     /locus_tag="VFJ72_12020"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET7480231.1"
                     /translation="MESVNGPNHIEGNLSAAGKSFGIVAARFNDFIVRPLLDGALDAV
                     RRHGGDVSLVDVAWVPGSYEIPLAAKQMAHSGRYDAVICLGTVIRGATAHFDYVAGGA
                     AGGISSAMLETNVPIIFGVITTETIEQAIERAGTKLGNKGFEAAVSAIEMADLMSRLE
                     DRTPEAGLSHTRPEADYSRS"
     gene            complement(20844..21068)
                     /gene="rpmB"
                     /locus_tag="VFJ72_12025"
     CDS             complement(20844..21068)
                     /gene="rpmB"
                     /locus_tag="VFJ72_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET7480232.1"
                     /translation="MAKICYSCGKAPSFGNARSHSLRATRRRWNPNLQKVRIQVGTAT
                     RREYVCTSCLKAFKVQKAEVNRRPAVAQSV"
     gene            21264..22853
                     /locus_tag="VFJ72_12030"
     CDS             21264..22853
                     /locus_tag="VFJ72_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET7480233.1"
                     /translation="MAIRMAFVVAAAHAALKSQVARVNALNVYPVPDGDTGTNMLLTL
                     ESILKEAKDKTYESAEAASKAGARAALMGARGNSGVILSQMIRGACEVLAKRASLTAE
                     TFADALEGARERAYASIRQPVEGTMLTVIKDAATAAREAVRDRNVDLPAAVKAAAQAA
                     HASVRRTPELLGVLREAGVVDAGGLGVAIILDGIYACITGQKIEVDEEDESGTPDLDA
                     IHAEEEAWGYCTEFLVTGFEGDAGEFEDYIYASGKSVLVIPDDDVIKVHLHTQDPGSA
                     LTYAGTFGRLSGVKVDDMEAQVHARGDGSTESSAESASIGVVAASRGAGNRKLFESMG
                     AVVIEGGQGANPSAADFAAAVDKTGAPAVILLPNNKNIVPTAEQVGELVEAETYVVPT
                     TTIASGLAAMVGFDGEGEADDVAEEMCEILDTLRSAEVTRAVRDVRIGDHEVSEGAFI
                     GLLDGDLHAVGESVEEVALKLAEDILDDGADVITLLAGEDLDGEILSRISDRIKALDE
                     DLTVEAKTGGQPMYPLQMVAE"
     gene            22850..23665
                     /locus_tag="VFJ72_12035"
     CDS             22850..23665
                     /locus_tag="VFJ72_12035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7480234.1"
                     /translation="MTTAIVTDSTTSLAPELRERPDVRVVPLTFHFGPDETYLDKVDM
                     SNAEFYERLADSDEFPTTSQPPAGAFVEAYESLSVYDDVLVLTLSGKVSGTYESAVAA
                     AEMVDRPVEVLDTRSAEMGSGLILLEALKVIDEGGEFRDARRAAEAAIRRCSILFAVG
                     TLQYLAKSGRIGRAQRMVGTALDIRPVLALEDGEVVPHKRARGRKRQMAALVEQLQPA
                     VEAGSSLYFGHIDAPEAVSELAAALGVEQERVAEIGGVVGCHVGPGAYGVAYL"
     gene            23646..25817
                     /gene="recG"
                     /locus_tag="VFJ72_12040"
     CDS             23646..25817
                     /gene="recG"
                     /locus_tag="VFJ72_12040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET7480235.1"
                     /translation="MAWPTSSSGAAPGQTPARLNLPERTTLTDLRARPVTGLPGVGPR
                     IAAALKDLGIESLADLVSHYPSRHEDLSNVKKISDLRVGERATVIGRIVSTRPVGKPV
                     RGRSPGFSAQLYDGTGYIPATVWGRGWLQGQLVPDTWVLVSGEVQRRYGIQLAAKSLE
                     VLDDRDAPGDGVSGPHAGRFVPVYPVNKGIQTRRMRTLIHRALDEAGRILDPLPAEVL
                     VRHRLPNLHDAMHEVHFPSEKKRLKSAMRRLIFHELFMIQTGLAARKARIERTERGGR
                     HIGDGSLLNPFLKGLPFGLTGAQERVIKEVLADMRSEKPMRRLVQGDVGSGKTVVAVA
                     ALLSAVESGGQGALMAPTEVLAEQHYLSVSSALAGLPVRVALLTGSQGAAERREMLEL
                     VRSGEANIVIGTHALIQKGVEFRDLSLVVVDEQHRFGVGQRMVFKEKGAMPDMMVMTA
                     TPIPRTLSLTLYGDLEVSVIDELPPGRKPVETVLMDLSGREEAYEAVRRELEDGRQAY
                     VICPLVEESEALEDVRAAEELYDELAEEIFPDRKVGLLHGRMKAQEKREVMAAFRAGE
                     IEVLVATVVVEVGVDVPNASAIVIEGAERFGLSQLHQLRGRVCRGLHPPKCFLIGEPK
                     TDDSRSRLEALCEHQDGFKLSEVDLAIRGEGTLFGSRQSGMPDLKVAKLLRDIDVLVE
                     ARREAFELVAKDPTLKAPVHRPLRREIRELLGADVEWLFRE"
     gene            25825..26889
                     /gene="coaD"
                     /locus_tag="VFJ72_12045"
     CDS             25825..26889
                     /gene="coaD"
                     /locus_tag="VFJ72_12045"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET7480236.1"
                     /translation="MRVIAGEARGVRLTNVPPGVRPTADRVRESLFNRLGQFFDGGEV
                     LDLYAGTGALGIEALSRGCERAVFVEKDRRAVAAIRENLRRTGFLERAEILRDDVGRA
                     LDALEGRGGQYNLIFIDPPYRIAALEVGSVLMRLRAILAPNGRAVVETGGLSPEVIES
                     KKGESRRYGGTVVTIFERSDLTMNVAVCPGSFDPITAGHLDIIRRAAGIFDHVVIAVG
                     RNLRKGPRLTASERAHLIETVTRDIDNVSVELMDGLLVDFAREQGARVIVKGLRAVSD
                     FESEFEQAQLNRNLSPEIETVFIMSAAEYSFLSSSAVREIAGLGGDVRGLVPDVILDV
                     VREIYSGSGEKTETTRQRIG"
     gene            26894..27358
                     /locus_tag="VFJ72_12050"
     CDS             26894..27358
                     /locus_tag="VFJ72_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HET7480237.1"
                     /translation="MDVLVLIDRLEELVEEARSFPGFGNTAMVDRDAAFDVIDQMRQT
                     IPEELKQARWIVKERQAMLDEARSESDRIIRGAQEEAEKITSEEEVLKRAEKRSTEIM
                     EDARRREREIRLGAEDYADEVLANLEDNLAKLLEAVQRGRSKLQGGEEEQAR"
     gene            27368..27826
                     /locus_tag="VFJ72_12055"
     CDS             27368..27826
                     /locus_tag="VFJ72_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480238.1"
                     /translation="MSNSIPLRRPRAEVGDRYEQRASFTVPAFDLYGRHHEIENAEAD
                     VAVTRLAEGLHLDLEVRVVLSTTCDRTLEPTEVELLFGESEFLPGPNNRELCIREWEL
                     DLVGYTERALPSEVPMQVFHPGTTAVRPEESAEETDPRWRGLGGLFASGF"
     gene            27857..28042
                     /gene="rpmF"
                     /locus_tag="VFJ72_12060"
     CDS             27857..28042
                     /gene="rpmF"
                     /locus_tag="VFJ72_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET7480239.1"
                     /translation="MAVPKKKTSQARRDQRRAHHALKSANLVPCPTCGYPRVPHRVCP
                     ECGSYKGRNVVAVEASE"
     gene            28061..29074
                     /gene="plsX"
                     /locus_tag="VFJ72_12065"
     CDS             28061..29074
                     /gene="plsX"
                     /locus_tag="VFJ72_12065"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET7480240.1"
                     /translation="MRIAVDALGGDNAPGEIFAGTLAAARRLPEDEFILVGASGGTQT
                     FAGAREVPPNISFRESGGAIGMEEEPAAALRSRPDASVAVAARMVRAGEAEAFFSAGS
                     TGATVAAALLSVGRLRSCRRPAIATVLPFPSPVLLLDAGATISCRAHDLVNFAILGSV
                     FAKRYFRLGGEARVGLINVGEEPSKGTDLTKEAYGMMRDSGAIDFVGNVEGRDIGRGV
                     ADVLVTDGFTGNVVLKTAEGVASEILQMVRSAVTVGIVSKAAAAVLRPRLIGVRDQVD
                     PENYGGSFLLGVRGSVVIGHGNSEARGVENALLGISRTGAGLAAVLEEALAASQTKEA
                     EHS"
     gene            29071..30018
                     /locus_tag="VFJ72_12070"
     CDS             29071..30018
                     /locus_tag="VFJ72_12070"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET7480241.1"
                     /translation="MSGAPVGKIVGVGRALGGRVVTNHDLENVLDTTDDWVKTRTGIR
                     ERHFVAEGETCATLATDAAVAALKHAGVTGESLDLILCATSTGPESMPSVACLVGEAV
                     GAPGVGAMDLAAACAGFSYAASVADSMMRGGIAERVLLIGADAMTAVVDGNDRATAVL
                     FGDGAGAVVLERGEGESGFVDHILGADGRMASAGRAGHPGDEQKLYQNGREVFKFAVR
                     ILPEMVEKLVARNGISLDDVQYIIPHQANARIIQAAARKLEIPEERIVINVDHFGNTS
                     AGSIPISYPDIYDDFEPGKYIITVGFGFGLTWAANLYRI"
     gene            30046..30864
                     /locus_tag="VFJ72_12075"
     CDS             30046..30864
                     /locus_tag="VFJ72_12075"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7480242.1"
                     /translation="MQIRRAIVFPGQGTVGGEVSGGIQDAVQRQVGDGEVPYQLSVFA
                     SSVHLLGRLREHGIKPNVVAGHSLGEYAAAHAAGSLGLEDGVRLVAERDRLMNEASRE
                     NPGAMVALIKADPMDVVSAVEEVEGVVVAANFNTPRQTVISGEEEAVQAVADSVKGRK
                     VRLDVAGAFHSPLMLEASRAMSRLLLEVELRKPEIPMVSAVDGAILATVDDLRQALTN
                     QMLSPVRWVAVIERFRDLGVREMVEAGEGGTLTRMLRDFPQDRLKGMTAKEALA"
     gene            30861..31310
                     /gene="fabZ"
                     /locus_tag="VFJ72_12080"
     CDS             30861..31310
                     /gene="fabZ"
                     /locus_tag="VFJ72_12080"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET7480243.1"
                     /translation="MKAPLGPAEIRALIPHRYPFLLVDRIEEIEPGIRAVGIKNVTQN
                     EPFFQGHFPDYPVMPGVLIVEAMAQVGAVGVMAVEEYRQKLALFAGIDGVRFRRQVVP
                     GDVLRMEVEISRLKGSVGRGKGRATVNGERVCEADLMFAFAARGEVE"
     gene            31307..32053
                     /gene="fabG"
                     /locus_tag="VFJ72_12085"
     CDS             31307..32053
                     /gene="fabG"
                     /locus_tag="VFJ72_12085"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET7480244.1"
                     /translation="MIESGRVAVVTGGGRGIGRAIAVRLAEEGANVAISYRSNDSAAE
                     ETAAAVREAGVECELFKGDVAEPDDVAALFKGAQEKFGRVDILVNNAGTTRDNIMLRM
                     KEEEFDEVLRTNLKGTYLCTRAVLRTMVRARWGRIVNVSSVVGLVGNAGQANYAASKA
                     GIIGFTKSVAREVAQRGITVNAVAPGYVETELTGKLPDTVKDQIKAQVPTGRFGEPEE
                     VAEVVAFLAGERAGYVTGQTLAVDGGMTMQ"
     gene            32124..32396
                     /gene="acpP"
                     /locus_tag="VFJ72_12090"
     CDS             32124..32396
                     /gene="acpP"
                     /locus_tag="VFJ72_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7480245.1"
                     /translation="MEESQRRDRVSRDEILAKIQEITADRLGVDEGDVTPDASFREDL
                     EADSLDLVELIMELEEQFGMEIPDDDAEKITTVEEAVDYVSEHQAA"
     gene            32393..33661
                     /gene="fabF"
                     /locus_tag="VFJ72_12095"
     CDS             32393..33661
                     /gene="fabF"
                     /locus_tag="VFJ72_12095"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7480246.1"
                     /translation="MTQGASNGKWAGNGHALITGTGAVTPLGTGTENFWDAALHGKSG
                     VGEITLFDSSPYPSHIAGECSDFDPTDFMSKKEARRMDRFAQFGIAAGIQAAESAGIV
                     DVIRERPERAAIVMGTGIGGLSTWEREYKVLNERGAGRVSPFLITMMVPNMAAGQLAI
                     MLGITGPSQCPVLACATGGEAIAEGLELIRRGDADVVIAGSSEAPITPLSMAGFCAIR
                     AISTRNDEPQRASRPFDAGRDGFVMSEGAGAVVLESPEHAARRGAEPIAAVAGYGRST
                     DAYHVTAPDEQGRGVERAMHAALKDSGLTAEDIGHVNAHATSTPTGDGPETKAISRVM
                     PHALVSGTKSMTGHSFGAVGATEGIMCALAIKNKIVPPTLNLDDLAEDCEPLNYVGSE
                     AQKAPDLRAAISNSMGFGGHNVVVAFRDVE"
     gene            33673..34332
                     /locus_tag="VFJ72_12100"
     CDS             33673..34332
                     /locus_tag="VFJ72_12100"
                     /inference="COORDINATES: protein motif:HMM:NF025973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HET7480247.1"
                     /translation="MSIKDLIAILPEPLRQRSLTHPSVADPYDSNGRLEFLGDAILNT
                     RVAELVFHSYPELLEGDLTRIRAHLVRKTFLATVGRGEGLEEIIESSVINDSVIADTV
                     EAILGAAHLIDRDLVARTIDEIFNPSEVDTDELRDWKTLLQETLQADGLRPTYHVLSK
                     QGPPHRPNFVSEVSVDGRNVGTGEGHSIKESEQAAARAALEILGIRPLPRSAVEVHVG
                     A"
     gene            34414..>36288
                     /locus_tag="VFJ72_12105"
     CDS             34414..>36288
                     /locus_tag="VFJ72_12105"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7480248.1"
                     /translation="MLSAVYIKGFKTFARPVRMPLEGGLTAIVGPNGSGKSNITDAVL
                     FAFGEQSPGVLRAGVMGDLIFSGSETLPASGVAEVTLVLDNESGEISLPYQEVSISRR
                     ISRNGDTEYRVNGSRARLADVRAVAGEAGLGRHSILRQGAVDAIVAGGAAACRLALEE
                     AAGLGVYRRRRLSASRRLEKAQAQLDQSRQLEAELASQLRRIEREAVAAREYREVEGR
                     YRKLSLAHLYRVADQGLDERRKQLERSEKSVVALSEQERALREEEAGISPQTQRLESA
                     MRSLERQAEELEEAEEDLRAETMRADRALLRLESRAGGTGDRQRNIATLEAELERIER
                     ALGAMRRELDLLEIEHTRKREKQTELRRMLDERRQRQGEADRSRSRIAAELEGLRGRR
                     ERILARGTKSGNVGEEYLRELAEVSGEIERLTETGFAAPGRNIRDTLGGLRAALDERA
                     AGINRRGGALEAALGAAEAVIRSLRDADTQDAGGPRLHEVIRARPGFEGAVEAALGDA
                     AGGLLAANVSEGIKLLSEGEPVALRLDARPVDHYADHPGLPLLDCVELVDEEYAEAVE
                     RLLAGIYVVEDPAHDHPENGHVVVTRNGFRLTRTSVRLRSEDGRFSRKARLDREQNRL
                     R"
BASE COUNT         6652 a        10870 c        12916 g         5850 t
ORIGIN      
        1 gaccgtgata ccttagcact ggctcataga gggagcagcg gttccgtagc gggctcccgg
       61 tcggtttgca ggggagagtt tatccgagac tacggggttg gaggctggcc cgccagactc
      121 tggttatgtc tggtgatggt attggccgga ctcttcttcg ccggatgcgg tggcggagag
      181 gagccagctt cccctcctcc cgcgaccacg acggcgcccg cggcggctcc cgccgcgcct
      241 cctgcgggcg gcacgacggt cgggtcggga caggccgaga cgatccggga ccgcccaatc
      301 tccttgaaca tggaccagcc ggtgccgccg gacttcaaga cagcttatca gcggcgcgcc
      361 ctgatcgcgg tagagtttta caagacggga cagaacccgt tctatcctca agggctcggt
      421 gtggactctc aggtgaacac gttcgtggac cagctccgga ctcagtaccc gaccgtagag
      481 ttctttagtt acaacataga taaccccggc gccgggggcg acaacgtgaa gcttcagagc
      541 gggcagtatg ggacgctcgc ggctcagctc ggggtcgggc tgaccccgtt cctggcgacg
      601 ctcgctccgg cgtcgaacgg ggacggctac atcataaaga acctcttcca gggctacgtg
      661 acgcagcccg tgatcagcca ggcgctcttc gacctttcgg ccgtgcaggt tgaggacaac
      721 acgagcgacg tggatgtgaa gatagaccaa ctggagctga ccgagcgggg aggcggtatc
      781 gaatacatca ccgtcaggaa tacgagcccg gacccggtga acctccaggg gttcacgctg
      841 cggacgctcg acccggagac gggacaggtg aacgaccagt ccgccaccgt cgagataaac
      901 gactcgatcg aggtccagcc cggaaagagc gtctccgtcg gccggtcggc cgaggtagta
      961 gacgcggacg gcaagaaggt tgccggggta ttcgtgggtg gagaagacct ggggctggcg
     1021 ccgggagatc aggtcgccct cctcgacgcc ggaggcgccg tcgccgccac gatcacggtc
     1081 tgagatccag ggattccgtc tgcataagcg ggcctgcaaa ttcgcccgct tacgctacac
     1141 taatcgtgta tgagcgactc tgtctacctg gataacgccg ccacgacgcc gctggactcg
     1201 cgagtcctgg aagccatgct gccacacctg cgcggacggc gcggcaacgc ttcctcgctg
     1261 cacgcctccg gggcggcggc gcgcgaggcc gtggaggagg cccgcgagtc ggtcgcggcg
     1321 ttcctgggag cgtcgccgga ggagatcatc ttcaccagcg gcggcacgga agccgacaac
     1381 atcgccgtcc tgggactcgc ggttgcccgg ccggagaagc gtcacgcagt cgtctcgcgc
     1441 atcgagcacc ccgcagttcg tggagccgcc gcgcgcctgg aaagagacgg ctacgaggtc
     1501 tcctggatcg gggttgacga gaacggaatg gtggacccgg agcagtttgc ccggtcgttg
     1561 cgaccggaca ccgcgttcgc ggcggtgatg tgggcgaaca acgaggtcgg gaccgtgcag
     1621 ccggtcgagg aactggccgc gatctgccgg gagcgaggag taccgctgca cgttgacgcg
     1681 gtgcaggcgg cggggagaat tcccttaaat acggcctcgg tccctgtctc gacgctggcg
     1741 gtctcggggc acaagctcta cgggccgcag ggggtgggcg cgctgtacgt ccgcgagggt
     1801 gtggatctgg agccgacggt attcggcggc ggccaggagc gcggcctgcg gagcggcacg
     1861 cacgatgtgg cggggatcgt cgggcttggc gcggcggccc ggctggcgtc agaggagttg
     1921 gaggagcgcg cgtcccacga agcggcgctc cgggaccgta ttttggaggg tgtccgtgag
     1981 aaagccggcg tgcggctcaa cggtcacgcg cgggagcggc tctcgaacaa cgtacacctg
     2041 tatgtggagg gggtcgaggc tgaagggctc gtgctgttct gcgatgctct ggggttcgag
     2101 atcggcagcg gctctgcctg cgcttcgggc gggcacaagg cctccccggt gctgctggcg
     2161 atgggccgga gcgagcagga ggctttctcc gcggttcgaa taacggtcgg caaggacaac
     2221 acggaggggg agatcgaagc tttcgtcgaa gcgttcgccg cggcggtcgc caggcttcgc
     2281 gagctatccc cactatacgc gggctgatac ccgatggccg aacgctaccg cccacaggtc
     2341 atcgaacacc tcgtaagacc tcgccacgtt ggcgacctgc ccgaacccga tggcgcggga
     2401 gagtcgggag actccgcctg cggcgacatc gcccgcttca cggtgcgtat cgaggagaac
     2461 gtcgtccgcg aggtcggcta caaggtctac ggatgcgccg cgtgcatagc ggcgggctcc
     2521 gcgctggcgg agatggtggc gggagaggat ctgctgcgcg cggcgagcgt ctccaaggag
     2581 gatgtgcagg acgcactcgg cggaccgctg cctcctggca aggagcacgc cctgacgctg
     2641 gtcctggacg cgctgcacaa ggcgctggag gactacctca accggcgggc cggttcgatg
     2701 ctggtggatg ggtacggagg cggacgcgct ggcgagaaga agacggtcgt cgcggcgatg
     2761 agcggcgggg ttgactcggc ggttacggcg ctcttgctca aggaggcggg ctacgaggtg
     2821 gcgtgcgtga cgttccggct gcacgacggc gagcgcggga gccggtcgtg ctgctcgccg
     2881 gacaccgtgc tcttcgcccg cgataccgcg caccggatgg gtcttccgca cttcacgctc
     2941 aacctcaagg agcttttcaa ccggcgcgtg atgcaggact tcgtcggtac ttatgaagcg
     3001 gggaagacgc ccaacccgtg cgtcgcgtgc aacgcccacg tcaagttcca cgcggcggcg
     3061 ttcctggcgg acaggctcgg cttcgaccac gtcgcgaccg ggcattacgc gcgtgtcggc
     3121 gtcgggccca cgctcgcgcg tcctgttgat gagacaaagg accagaccta tgttctgtgg
     3181 cccatgccga aggagttgct ggcccggacg gtcttcccgc tcggcgagta caggaagacg
     3241 gaggtacgca agatcgccga ggaccggggg ctcgcggttg cctacactcc cgagagccag
     3301 gacatctgct tcatcccgga cggcaactac cggggcttca tccgcaagaa ggtcgccgcc
     3361 gagccgggcg agatcgtgga tactagcgga gaggtgctgg gacgccacgc gggggtagtg
     3421 gacttcacgg tcgggcagcg gcggggtatc ggcgtctcgg ctccgacgcc gctctacgtt
     3481 acggaggtac ggcctcagag caagcaggtc gtcgtcgggc ggcgcaggga cctggaggtg
     3541 cgcgaggtgc gcgtctccgg gatgaactgg ttcctggacc ctggcgaggc tcgcgccgtt
     3601 caggtgcgct acaacagctc gccggttccg tgcgagatcg aagacgtggg cgacggggag
     3661 tgggtcgcgt atctggagga gcctgtaatg ggcgtggcgc cgggccagtc ggcggtcttc
     3721 tacacgggag acggccagcg cgtggctgga ggcggagtcg ttgcccggaa ccggccgaaa
     3781 gctgcggcgg ctgggggaca ggagatatag aatggtggtc cggtgttctt aagagaggtg
     3841 cgctgaactt tggatacgtt tgtggagctg atgaaagcct tgctcttcgc ggggatagcg
     3901 gtggcgttct tcgtgctggc gtggatgttc ttgaggctcg ccagtatctt ctcgacgacc
     3961 gagcagacga taaaggaggt ctccgagcag gtggtgccgc tgctcggcaa gacgcacgtc
     4021 acggtagaca acataaacag ccagctctcg caactcgaca cggcggtggg ggacgtcgcc
     4081 gggataacag gagaactgga ccagacgaca accgtcataa cgcgcggtgt gcggagtggt
     4141 gtaataaagg cttcgtcggc cacggcgggt ctctctcgcg gaatcagcac gttcctgggt
     4201 ggcaagaaga cgaccgaagg ggaggactag ccgttggagg ggtggggcaa gatcttgacc
     4261 ggagcagcgc tgggggtgct cggcacggtc tacgcgacta acgaggagtt ccgcaagcaa
     4321 ctgcccaaga gcgcccgcga actgccggtg gtggtgcgca agcgcttcga cgaggcggtc
     4381 tccgcggcgc gcgaggcgtc gtccagccgt cggcaggaga tcctccggga cctcgaagtc
     4441 cacggcggcg gagcgcacgc ggctcgcggc gtggagcccg atccggccgc gccggagaca
     4501 tccgtgccgg agccacccgt agtgactgat ccggacgcaa cccagcaact ctctcgcccg
     4561 acagaataaa ccgagccctc ggagcgaatc cgaggaagga gtcgatttga cgaccgaaac
     4621 ggatgccgag tacacgtatc tggagatccc gccgtcgccg gagatgcgag gatgcatcgg
     4681 gctggtgatg gccggtatgg cggcgcgcgc gaagatcggc gtgggcgggc ttgatgaggc
     4741 tgtggagata ctggagagcc tgcactccga gatcgagccc acggtgtacc gcttctcgat
     4801 ggatgaggat cgggtggtcg cgcagataga ggacccggaa gagggagcgt ctatatggcg
     4861 gacggtggtg gaactcgtat cgtgaaccgt cgttaccgtc gccctgacaa ggctcgtacc
     4921 cggcggctcc tcatcgctta ccacaaggag ggcgaccagc gcgcgcggga gcgtgtgatc
     4981 gcggagcagt tgccgctcgc ggagttcctg gcgcgcaagt tcgccggacg cggagagccg
     5041 gttgacgacc tcgtgcaggt cgcgtcggtc ggtctgatca aggccgtgga ccgtttcgac
     5101 gtggaccgga acatcgagtt ctcgacctac gcgacgccga atatcctggg tgagatcaag
     5161 cgctacttcc gggataaggg gtgggcgatg cgcgtccctc gcggccttca ggagttgcgc
     5221 cagtccgcca aggaggcgat ccaggaccag acgatcaaga ccgggcgctc gccgaccgta
     5281 gaggagctgg cgaagatgct ggactccgac gaggagagcg tcgccgaagc cctgaccctc
     5341 ggacgcgcct acaacaccgc cagcctcgat gctccggtga accaggaaga taccgacggc
     5401 gacacgatca tagacctcca ggccgacgat aacttcccga tagagggcct ggaggacaag
     5461 atcctgctcc agagcgccat gcacggcctg aaggagcagc agcagcagat cctcaagctc
     5521 cgcttcaacg agggcaagac gcagaccgag atcgccgacc agatcggggt cagccagatg
     5581 cacgtctccc gtctcctgcg ccgcgccatc gacgacctgc gcgtccggct cggcgagatg
     5641 gagaagaacg ggaactagaa aacgcccgcc atccggttac aataccccga catgacgccc
     5701 atgcagagca gtgaaatccg ccagaagttc ctaaatttct ttaccggtag agaccaccgt
     5761 ctctacccca gctcgtccct gatcccgcac aacgacccca cggtcctcct cacgaccgcc
     5821 ggtatgcaac aattcatcac ctacttcacc ggagaggacc gtccgcccag cccccgcgca
     5881 accagcgccc agaaatgctt ccgcacccag gatatcgagg atgtcggcga ccccacgcac
     5941 ctgaccttct tcgagatgat gggcaacttc tccttcgggg actacttcaa gaaggaagcc
     6001 atcgagtggg cgtgggagtt cctgacgcgg gacctgggac ttgcgccgga gcggctctgg
     6061 atcacgatct tcgagggcga cgaaaacgct cccgaggacc tggaagcgaa ggacttctgg
     6121 atgaaggtcg gtgtgccggt agagaggatc ttcggcctcc ccaagagcga gaactggtgg
     6181 ggaccacccg gcgactccgg tccctgcggt ccctgctccg aggtctactt tgattacggc
     6241 gaggagtacg gcgagggtga cccgctcgcc gacccgaagt acggccccgg cggcgacgag
     6301 ggagactcgc ggttcctgga gatctggaac ctcgtcttca accagtatga gcagcgtaag
     6361 gacggcaccc tcaccccgct cgcgaaaccc ggcatagaca ccggcatggg tctcgaacgg
     6421 accgccgccg tcgtacaggg cgtccacacc gtctacgaca ccgacctcta cgccccgatc
     6481 ttcgagaagg cgcgcgagat ctccggcgcg gagcgcggct cctccgatga taccgatcgc
     6541 gccctctaca taatcgcgga tcacgtccgc agcatcgcct tcctcatcgc cgacggcgtc
     6601 cgtcccggta accagcgccg cgagtacgtc cttagacgca taatacgccg cacggcgttg
     6661 caggcatatg cgcggcttgg gatgaagccc gagcaaatcg cggatctggt cgaggttgtg
     6721 gtggattcga tgggcgacca ttacgaggag ttggagcggg cgcgggagga catccggcgc
     6781 atcgtgacct ccgaggctgc gcggttcgtg gagatctacg actccggcat ggggttgctg
     6841 gaggcggaga tcgagcgtgt cggcggcggg agcttccccg gcgaggtcgc gttcacgctg
     6901 cacgacacct acgggtttcc ggtcgaggtt acgcgggagg tgctggcgga gcgcgggatc
     6961 tcgctggacg aggccgggtt cgagcgcgcg atggctgacc agcgcgagcg ggcgcgggag
     7021 gctatgcagg gctatgaccg ggtcgtggcg gcgttccggg accaggagat aaagagccgg
     7081 ttcgtgggct acgagcgcga gcaggtcgag actcgaatag tcgctgtgga gccggtgccg
     7141 ggagcggaag acgaggtctt cgtcgtgctc gcggagaacc cgttctacgc cacgggcggc
     7201 gggcaggtgg cggatgaggg gtggatctcc tcggacaacg gccagttaga ggttgtcgac
     7261 accatcccag ccggtgacta tcaggtgttg cgcgccaggg tggagcacgg cgacttccat
     7321 gcgggagact ccgtgacggc ttccataaac cgcgtccggc ggcagcagat agaggccaac
     7381 cacaccgcca cgcacatcct tcactgggcg ctgcgggcgg tgctcggccg ggacgtggtg
     7441 caggccggga gctacgtcgg accggaccgg ctgcgcttcg attaccggta cgccggcaag
     7501 gtgagcgacg acgagttgcg gcgcatccag gagctgtgcc tgctcaagat caccgagaac
     7561 cagcccgtgc gctacttcac caccacgatg gacgaggccc gtaacctcgg ggcgatgatg
     7621 ctcttcgggg agaagtacgg agacctcgtg cgggtcgtcg aggtggacgg gttctcgcgc
     7681 gagctgtgcg gcggcacgca cgtgcgggga acggccgagg tcggggcgtt caagatcctc
     7741 tccaacagga agcacggcgc ggacctgtac cggatagagg tcatcacggg tcgggaggcc
     7801 ctctactacc tcattggggc cgccgagcgg gccgaggaag tctccgaggc gctccgggtc
     7861 gagttggacc gtctgccgga ggccgtagag aacctgcgcc agagcgcgcg gcaggcgcgg
     7921 gagatggagc gcgagcaggc gttgaagcag ggacttcagg aagtcggggc gctcgtcgag
     7981 agtgcggagg ccgtggacgg catgaaggtt gtcacggggc aggtcgtcgc gacggacgtg
     8041 aaggggctgc gccagatctc cgacgacgtg aagaaccgtc tcgggggtcc agcggcagta
     8101 gtgctcgccg ctgaccttgg cggcaaggcc gtgctcatcg cgaaccttca cccgcaggtg
     8161 tcggaaaaaa tagaggctgg cgagatcgtg cgcgaagcat ccggcgtgct gggcggcggc
     8221 ggaggcggcg gaccgacgat ggctcaggcc ggcggtggag acctctccgc catcccggac
     8281 gctctggata aagtccgcgt catcctccgc gggcggctcg cgggccggga aggctaggct
     8341 catgcaggct tcgagccgcg ctacagggag aatcgccgcg ctcgatctcg gcgaggtccg
     8401 taccggggtg gcgttatccg acccctcgcg gaccatagcc cgtccagtgg aggtcgtcgc
     8461 gagcgagaaa ctggaggagt acttgcgagg gctcttagaa gatgagggcg tcgaggagat
     8521 cctggtcgga gtcccgaaga cgctcggcgg tgaggtgggg tttcaagcgc ggcgcgtttt
     8581 agctacactg ggccacctga aaacggtctt cgccgctgta acgttcatcg agtgggacga
     8641 gcggttcacg acccgcatgt ccatcgccgg tcctggcggc ggtagcacag gcaggagcag
     8701 gaagagggac cggacgtccg ggactggcgg gagggtggat cacctggccg cggcccgcat
     8761 gttgcaggag tacctggaaa cgagggggaa cctttgaggc gctaccagaa gggaagacgg
     8821 agagaccgcg aggatcttga ggctcgcctc aacgcgcttc agccttcccg ctcgaagtcc
     8881 cgccgcaagc gccgctcgaa cgccggtccg gcgatcctgg gcatattgct cgtcatcggc
     8941 gtgctcggcg tgatctatct gatctacacc tccgcgacca gcggcagcgc gtcccaacag
     9001 gccgatacca gggcggggaa ggtcgaggtg gtgaagggcg acacgctctc cagcgtcgcc
     9061 gataagctcg atgaggccgg ggtagtaagc agcgcgacgc tcttcaagct ggaggctcgt
     9121 cttagcggcc agagcaccga gatcaaaccc ggcgagtaca ccttcgggcc ggatgacgac
     9181 actgatgcga tcatgaagaa gctgactacc ggcaaggatg tcccgacata cgccataacc
     9241 atcccggagg gcctcaccct gcaacagacg gcggagacag tggcccagag caagagcggt
     9301 atctcggcgg cggacttcga gaaggcggct aaaaagaccg actacggcta cgctttccta
     9361 aaggatccgg cgataaagtc caccgagggc tacctcttcc caaagaagta cgatttcgag
     9421 aaggggacca ccgcgccgca gatagtcaac cggctgctcg aacagtacct gctggagacg
     9481 caagagctgg acttcaaggg ggccgaggac cggctgaacc tgagcgagta cgagttggtc
     9541 accgtcgcgt cactcatcga gaaggaagct gccagccccg aagagaagcc aataatcgcg
     9601 tccgtcatat acaaccgcat gcggcagggg atgccgctgc agatagacgc cacgatccag
     9661 tacgcccgcg gcaagccgaa ggaagatctc tccctggacg acctggagat cgactctccg
     9721 tacaacacgt acaagaaccc ggggcttccg cccggaccga tctgcagtcc cagccgggag
     9781 tcgctggagg ccgccataga gccgaaagag acggactacg tctactacgt gctcaaggcc
     9841 aacggtcagg agcactactt cacgaacaac tacaatgact tcttgcaggc taaagccgag
     9901 gctggccggt agggtcaggg accgctccgc cagataactg agagccgtcc ccgtgttgag
     9961 cgcgccgccc tccgaataaa gcgcgccaat atgctcccct gttgccgtta cggtgtatcc
    10021 tgaagaggcg atggatgctc tgagtggaaa gtacgggtgc tggttaacgc aacttataag
    10081 gggatcaagt ctgacagccg acttgtttgg ctccattcaa ggcgcggaga actcgccctg
    10141 tgatggagct atccgggagg tgagcgatgc tctcacgcga gggtaaccat ccggctgcgg
    10201 gtccggcgcg ggaacttgga gagtacctgg tggcggcggg cctgatctcc gaagggcagg
    10261 ccgacgcggc gcgggacagg tgcagggcgg aacgagggaa cctggcgaag tcgctcgtct
    10321 cgctcggcta cctgagcgct gatgaggtgg cgcgcgctct ggcaaaggtg cagaacgtcg
    10381 agtacgtaga cctctccaaa gagagcctcg accctgattt gatcagtctc atcgcagaag
    10441 acgtactctt gcgtcacggc gcggtcccgc tccgggcaga gagcggacgg ctcatagtgg
    10501 cgatggccga tcccggcgac accgaggcgc ggagcgacct cacaatcagc gcgggccacc
    10561 cggtcacgcc ggtagtcgct tccgaggagg agatccgccg gttgcaccgc aggcatttcg
    10621 gtactcgcgc agaaccctct tcggctgccg cggaagcgcc tcccagcggg gacaacggca
    10681 gcgttagaga gacccggagc agctatgccg cttcgccgcg cgagaggccg ggcgcaaggc
    10741 gtccggctgg caggacgccc aagatcgggg agatcttaat ctccgagggc aagctctcgg
    10801 agactcagtt gcaggaagcg ctgacgatac aaagaaacga ccccagggac ctcggagaga
    10861 tcctgatatc cctgggactg gtggaggccg aggacctggc gcgggcgctc gcccggcggc
    10921 tcaagctgga cttcgtggtc atctccgagc tgtcggaatc ggaggtggac ccggacgttc
    10981 tgaacctcat cgaggaacgg actctacgga agtacaccgc tctcccgctg cgcttcgagg
    11041 acgggcggct ggtcgtggcg atgagcaacc ccaacgacct cttcgccctc gaagacctgc
    11101 ggatcatgac gaaacatcag atcacgcccg tagtggcaac cttcgaagac atctccggcg
    11161 ccctgtcttt cgtgttcggg gacgaagagg ctctcgattc caagctggat gaaggcgggt
    11221 cttctataga ggcttacgag atggacgaac ccgcccctga gccggagttt gcgcaagctc
    11281 cgcctacaga gcttgcggaa gagccgccca cggaagagcc ctcaccggtg gatgagtcgc
    11341 cggagccgga ggccggggtg gatcaatccc gggcgtccgg tgacggggag tggccgcctg
    11401 agacaccgga accggctgaa ggcgcgccgg aggctggcgg ggccagccgc ggcgcggcgg
    11461 ttgaacgcgg ggctcgccgg ctcggtggag cggggaccag gatcggggat atcctacttg
    11521 cggaaggcaa ggtctctgcg ggacagcttg aagaggcgca actgacggcg cgggagcggc
    11581 gacaggatct cgggaagacc ctgctctcgc tcggctacgt cagcgaggag gaccttgcgc
    11641 gggcgttggc ggcccggctc cgcctggagt ttgtggagtt gacggagcgc gacgtggacc
    11701 gggccgcggc gagcctggtg gatcagcggg tgctgcgccg gctgggagcg atgccgctcc
    11761 gggtctcgga cggccggctc gtggtggcga tgagcgaccc gacgaacatc tacgccctgg
    11821 aggacttgag gatgatctcc gggtaccccg tgacgccggt ggtggccctg gagaccgaga
    11881 tccggcgggt ccagaataag ctattcgctg tcggcgaaga ggtcacggag cttcttcagg
    11941 aggcggccgg agaatcggtc ttcgaggacc acggcgaggt cgagcttggt ggggacgcgg
    12001 gagccgagga cgccccgata gtccggctcg tcggttccgt tttgcagcag gcggtgggag
    12061 agggcgcatc ggacgttcat atagagccgc gggccaggga actaaccgtc cgctaccggg
    12121 tggacggcgt gctgcgcgag gtcatgtcca tcccgccgaa gttgcagagc ggcgtgatcg
    12181 cgcgcctcaa gatcctggcg aacctcaaca tcgccgagcg ccgcgtcccc caggacggcc
    12241 ggttctccgt gcggctcggc ggccagaaga tcgacgtgcg agccgcctcg ctgcccacgg
    12301 tcttcgggga gaaaatcgtg ctgcggctcc tcgatacttc caacgtcgag gctaacctgg
    12361 agcaactcgg cttccgtacc gacatattcg agacttacga gcagatcttc cggcggccat
    12421 acggcgcgat cctggtgacg gggccgaccg gctcgggcaa gtccaccacg ctctacgcga
    12481 cgctcagcga gttgaactcc ccggagcgga acatcatcac ggtcgaggac ccggtcgagt
    12541 tcaggatgga ggggatcaac cagattcagg tgaatgccaa ggcgggactc accttcgcct
    12601 ccgggctcag gagcatactg cgcagcgacc cggacgtggt gatgatcggt gagattcggg
    12661 acttcgagac cgcgaagatc agcgtcgagg ccgcccttac cggccacctg gtcctcgcca
    12721 ccctgcacac gaataacgct ccctcggccg ttaaccgcct gacggatatg ggggtcgagc
    12781 cgttcctcac ttcctccgcc gtcgactgcg tgatcgctca gcgtctcgcg cgaaggcttt
    12841 gcgactattg caaggagccc gtagagatac cgtccgatgt gctggccgac atcaattttc
    12901 ctcatgagcg cgctttcacc aacggcctgc gcttccatcg cgccgtgggc tgcgaccgtt
    12961 gcggcggctc ggggtaccgt ggccggttag ggatatacga gatgatggtg gtgacgccgg
    13021 agatacggga cctcatcctg cgccgggttt cgaccggtga gatcagccgc gtcgccgagg
    13081 aacagggcat ggtgccgttg cgcgatgacg gtctcctgaa ggcagcggaa ggtctgacga
    13141 ccattgagga agtcctgcgg acggtggtct agtagcgggc cgcgggcgta taggtagtta
    13201 aagtatccgg gcggatgtga cgaatagtag aatgtaaccg aaggtatacg cgactagtcg
    13261 cagatatatg ggaggatagg actatgatgc aggaagccgg gctgaacgat tatctcttcg
    13321 atgcgatccg gcagggcgcg agcgacctgc atattacggt aggagtcccg ccgtccgtgc
    13381 gggtcagcgg gaagatccgg cacctcgatt atccggcgct cacacctaat atgacccgcg
    13441 agctgatcta cgacatcctc tcgaacgacc agcggcagcg cttggagagc gacctggaac
    13501 tggacttcgc gtacacgctc ccgcgcacgg cgcgtttcag ggtgaacgta tacttccagc
    13561 ggggttcgat gggcgcggcg ttcaggacga ttccgagtga gatccggagc ttcagcgagc
    13621 tcggcttgcc gaaggctatc gaggacatga ccgacaagcc gcggggactc gtgctggtaa
    13681 cggggcccac cggctccggc aagtccacga cgctcgcctc gatgatcgac aagatcaacg
    13741 agacccgcga ggagcacata atgagcgtgg aggacccgat agagttcctc cacacgcaca
    13801 agaagtgcat cgtcaaccag cgcgaggtga accaggatac ccggagcttc gcccaggcgc
    13861 tcaagcatgt gttgcgtcag gaccccgaca caatactcgt cggtgagatg cgcgacctgg
    13921 agacgataca ccttgccgtg agcgccgcgg agaccggcca cctcgttttc ggaacgctcc
    13981 acacccagga tgcgccacag acggtggaca ggatcataga cgtattcccg tctcaccagc
    14041 agtcccagat acggacccag ctcgccaacg cgctccaggg catcatcacg cagacgctaa
    14101 tcccgaagag ggacggcaag ggccgggtcg cggcctgcga gatactggtc cccacgcccg
    14161 gcgtgcggaa cctcatccgg gaggggaaga accaccagat ctactccgcc atgcagaccg
    14221 gagggaagtt cggcatgcag acgatggacg cggcgctcgt ggacctggta cgcaagggcc
    14281 tcatctcccg cgaagaggcc gagaagcgtt ccagcaaccc cgaagagttg aaccgtctgg
    14341 ccggcgggag catgggcgga gcgtccaacc ggcccgccgc gagttcctcc ggctcttacg
    14401 cggcgctccg cagataggga ggacgtaggt ggccgtatac acgtacaagg cgcgctcccg
    14461 tagcggcgag gtcctggagg accagatcga ggcgaacgac caggtctccg tcgcctccga
    14521 gctgcgccag cagggtcttc tcgtcataga catcaaggag cagggcgcgg cccagaagga
    14581 cttgctggag ccgttcaaga aggtcaagct ctccgacgta gtgatcttca cccggcagtt
    14641 cgccaccatg atcaacgcgg gcctgccgat agtgaggtcg ctctatgtcc tgggagagca
    14701 gacggataac cccaagttga acgaggtgat cgtggcggtc cgcaaggacg tggaggccgg
    14761 gctcgggctc tcggaggcgc tcgccaagca ccccaaagtc ttctcccgcc tctacatcga
    14821 gatggtgcgg gctggtgaga tcggcggcat cctagacggg gtgctgctcc gggtggccgc
    14881 ccagttggag aacgaccagg agctccggcg caaggtaaag agcgccatga cctatccgac
    14941 ggcggtgctc gtgatcgcgt tactcgcagc ggctttcatg ctgatcttca tcgtccccat
    15001 cttcgccaag atgttcgcgg atctcggagg gactcttccg ctccccaccc gcatagcgat
    15061 gggcgcctcg gacatcctta ccagcttcta cggcatactg gtcgcggccg cgctcatcgg
    15121 cggcgtcatg ttcttcctgc gttggaagaa caccgagcag ggccggaagg tgtggggccg
    15181 gctgatactc aagatgccgg tcaagatcgg agacatcgtc cagaaggtcg ccctggcgcg
    15241 gttcgctcga acgctcggaa ccctgagcgc ggccggagtc ccgatcttgc aggccctgga
    15301 gatcacggcc tcctcctccg gcaactgggt catcgagaac gctctgttgc ggagccgcga
    15361 ctccatccgg cagggaatac cgatctaccg cccgctcgaa gacgagccgg tcttcccgcc
    15421 gatggtcacg cgcatgatcg ccgttggcga ggagaccggg aacattgacg gcatgctcac
    15481 caagatcgcc gagttctacg agtccgaggt ggatgcgtcg gtcaaggcgc tgacttcgat
    15541 catcgagccc ttgatgatcg tggtgatcgg cggcatcgtg ggcggcatca tcatcgccat
    15601 gtacctcccg atgttcaaga tcttcgaact catcgaatag tggactcgct cgcaaccacg
    15661 cctctggaca acggccgggt ggcgcggttg gtcgagtctt accgggaaca gggggaccgc
    15721 cgggcgctgg agcgcctgtt cgagatgcac ggcaagctcc tcaaccacat cgtccgccgt
    15781 tacgcggcca cttctggaga gccatacgag gacctgttgc aggtaggcta cgtcgggctt
    15841 ctcaaggccg ccaacaacta cgccaccgat tcgacggccc gattcagctc ttacgcatat
    15901 tccatgatcg acggcgagct tcgccaccat ttccgcgaca ccgggatggt caggaagccc
    15961 cgctgggcgc ggagcctgta cgcgagggta tcggaggcga ccgcgaggct cacgggcgag
    16021 ctcggccggc cgccgctcct ggaggagatc tcgcgcgagg tcaacgtggc gcccgagggc
    16081 gttttggagc tgatgaagat cttcctcgac accgacgtgc gctctctcga tgacagtgcc
    16141 ggagaggcgg acctctcgaa catacgcagc atcgggtatg agacattctc gcttccgata
    16201 gaagaccgaa tacagctaga gcaggcgctc gataccctct ccgagcttca gaagaaggtg
    16261 gtctacctct tcttctacaa ggacctctcc cagaccgaga tcggaaagat gctcgggctg
    16321 ccgcagcgca agatctcgcg catcatcgcc gactctaccc gctctctgcg cgaccggctc
    16381 tcctcggata agtagctcgt taccgcctcg ggacaggctt ttctgtagaa tatcggcgct
    16441 gacgacacta ctcgacgagg gggaccgcat tggcgttctg gggtgaactt ggagacagga
    16501 tagtaggggc gccttcgatc ctcgcggcgg acttcgccaa ccttgccgag gaggtccggc
    16561 gcgccgctga gggcggggcg gagttgatcc acttcgacgc gatggacaac cactacgtcc
    16621 cgaacctcac cgtcggtccg gtcgtggccg cctcgctcac cgaggccgtc ggcctccccg
    16681 tggacgctca cctgatggtg gacaacccgg acaacctcat acagccgttt atcgacgccg
    16741 gggtagtaag catctccgtg cagccggagg ccgtcacgca cctgcaccgg acgctcacca
    16801 tgatccggga cggtggttgc gaggccggtg tcgccctcaa cccgaccacg ccgccggagt
    16861 tcatcgagtg ggcgctgccg tacctggact acgtgctcgt catgagcgtc aatcctggtt
    16921 tcggcggaca gagcttcatc ccggagatgg cggacaaggt acgcaggatc aaggagatga
    16981 ccgacctgcc cgttcaggtg gacggcggca ttaccgtcga gaccgctcca ctgatggtcg
    17041 aggccggggc gcaagtgctg gtcgccggtt ccgccgtgta tcgtggagac cccgtcaccg
    17101 agatgcgccg catcatcgaa gccggccgcg ccgctgccag gtaacggcct tccttgttga
    17161 aatcttgacc gtgtggtagc tttcgcctgt agctttcggg ggcgggtgga attcccgcac
    17221 cggcggtagc ccggagaggc gagccttttc cggggagccc gcgaaccctc ggcaggatcc
    17281 gggggttgag ccggtgaggg ccagcacttc ctggccgtag tccggcgccg acggttagag
    17341 tccggatggg agaaaggacg gcttggcagt cgaggccttc atggatcgcg cccgcgacct
    17401 cgcggagcgc ggacgtttta cagccgcacc caatccgctg gtcggggtgg tcatcgcgcg
    17461 cgacggcagg gttattggtg agggatggca cgcccgcgcg ggtcttgaac acgccgaggt
    17521 agccgcgctc tccgcgaacg ggacggatat gcggggcgcc tcgatgtacg tgacgctgga
    17581 accctgcaac caccacggcc gaactccgcc atgcactgac tccatcttgc gggccgggat
    17641 ctcgcgcgtc gtgatcggac acctcgaccc ggacccccgc atgcgcggac ggagcgtgga
    17701 gcttcttcga ggagcgggcg tggaggtcga ggtgctggac gatcccgcct tcgagcgcca
    17761 gaacgagcag ttcttccatt acatgaatac gggattgccc ttcgtgcatc taaagctcgc
    17821 ggccacgctg gacggcagga tcgcggcaag cggcggcgac agcaagtgga tcaccggcga
    17881 ggcagcgcgc cggcgcgctc acgccctccg ggctgaggcc ggggccgtac tcgtcggagc
    17941 tgggacggtc cgcgccgacg acccttctct aacggcccgc gatgtccccg accagccgcc
    18001 gcctctcact cgcatcgtcc tcgacccatc gttgagcctt gatccggaga gccaactcgc
    18061 ccggagcgtt gatgaagctc gcgtcctcct cttcgctgac aagacggtgc tggacggacg
    18121 ggagaagaga ctcgaagcgc tcggcgtgga ggttgtgggc gcgccgcgcg gcgagaacgg
    18181 cctggacctg gcttacgtgc tggcggaggc gggggaccgg ggcctgcgcg gggtgctggt
    18241 cgagggcggt ggcgagacgg cgggacgttt cctgcgaagt cgtttcgtgg ataaggtcac
    18301 ggtcttctac gcgccgaaga tactcggggc ggacggggtg cccatgatcg ggccgctggg
    18361 gatcaccagc gtgcaggacg ccccgcattt ctctgtgtca ggcgtggagc agcttggcga
    18421 cgacctcgcc gtgaccttct atcccaggcg tgaggagaat gatgttcacc ggattgattg
    18481 agactacggg ttgggtggtc tccctggagc ccggcgggat ggcgcgcctc gttatctccg
    18541 ccggcgaggt ggccgctgac gcccgcgtgg gcgacagcat ctccgtcaac ggcgtctgcc
    18601 tgacggtcgg ggagatggaa ggcgatacgc ttactttcta cgccatgccg gagacgctgc
    18661 gacggaccgc tctcggagac ctcaaggagg gcgccaccgt caacctcgaa cgggccatga
    18721 cgccacagag gcgcttcggc gggcacatcg tgcagggaca cgtggacggc gtgggttggg
    18781 tcgccggtgt ccggccggag ggggacgcgg agatctggga gtttggggcg ccggaatcag
    18841 tgctgcgcta caccgtcgag aaagggagca tctgtgttga tgggataagc ctgaccgtcg
    18901 tcggggtcag ggagagttct ctgacggtgt ccatcctgcc gcagacgaag gcggcgacca
    18961 acttgagcca actctcggtc ggggaccggg tgaacctgga ggcggacgta ataggtaagt
    19021 acgtggagcg gctgttgggg ccgtggatcg acagtgtaag agagaccgag aggagcttcg
    19081 gagatgccgt ttagtccggt agaggacgtt atagaggaca tcaagaacgg gaagatggtc
    19141 atcgtctgcg acgacgagga tcgggagaac gagggcgacc tgacgatggc cgctgagctg
    19201 gtgagcgcgg atgacatcaa cttcatggcg acctacggga agggtcttat atgtctcccg
    19261 ctcgcgggag agatcgttga ccggctggag atcccggaga tggtccagca caactcggcc
    19321 cggttgggaa ccgcgtttac tacctctatc gaggcgcggg acgggatctc gaccggcatc
    19381 tccgccgccg accgggctca cacctgccgc gtcgccgtgg gggacgagac aggacccgac
    19441 gacctggtga tgccgggtca catcttcccg ctgcgcgccc ggccgggcgg ggtgctgcag
    19501 cgcgccggac agacggaggc tgcggttgac ctcagccgtc tcgcgggcct caagccggcg
    19561 ggggtcatct gcgagatcat gaacgacgac ggcacgatgg cccgcgtccc ggaccttgag
    19621 aagttctcga aggaacacgg cataaagatg gtaaccgtcg cccagatcat cgaataccgc
    19681 aacacttacg agaaccacgt cgagttcgcg gtcgagacgc agctcccgac gaagttcggc
    19741 gagttccgcc tgcgcgccta cgagaatgac atggacgagt tgacacatct ggcgctcgtc
    19801 atgggcgagc ccgagggcaa ggagaacgtg ctcgtgcgcg tccactccgc gtgcctcacc
    19861 ggcgacgcgc tccactcgct gcgctgtgac tgcggcgagc agctcgacgc ggcgatggcc
    19921 cgaatcgctg ctgagggcga gggtgtcctc gtctacatgc aacaggaggg acgcgggatc
    19981 ggactcctca acaagatgcg cgcctaccac ctccaggaca agggcttcga caccgtcgag
    20041 gcgaaccagg agctaggttt cccgccggac ctccgggact acgggatcgg cgcacagatc
    20101 ctgaaggacc tggacctcca cagcatcaaa ctacttacga acaacctgac taaggtcgtt
    20161 ggcctgcagg gcttcggcct ggagatcgtg gagcgcatct cgctggagat aacgccgaac
    20221 ggctacaacg accgctacct cgccaccaag cgcgagaagc tgaatcacgt cttcgagagc
    20281 cactaggagg acattgtgga gagcgttaac ggaccgaacc acatagaggg taacctctcg
    20341 gcggcgggca agtcgttcgg gatcgtcgcc gcccgcttca acgactttat cgtgcgccca
    20401 ctgctggacg gcgccctgga tgcggtccgg cgccacggcg gcgacgtgtc gctggtggac
    20461 gtggcctggg tgcccggctc ctacgagata cccctggcgg ccaagcagat ggcccactcc
    20521 ggccggtacg acgccgtgat ctgcctgggt accgttatcc gcggcgcgac cgcccacttc
    20581 gactacgtcg cgggcggagc ggcgggcggc atcagctcgg cgatgctgga gacgaacgtc
    20641 ccgatcatct tcggcgtcat aacgacggag acgatagagc aggccatcga gcgcgcgggc
    20701 acgaagctgg gaaataaggg tttcgaggcg gcggtctccg cgattgagat ggccgatcta
    20761 atgagccggt tggaggaccg gacaccggag gccgggctct cgcacacccg gccggaggcg
    20821 gattacagca ggagctagag aggctagacg ctctgggcga cggcggggcg cctgttgacc
    20881 tcggccttct ggaccttgaa ggccttcaga caggaggtgc agacgtactc gcgccgggta
    20941 gcggtgccta cctggatgcg gaccttctgg aggttcgggt tccaacgccg ccgggtggcg
    21001 cggagggagt ggctccgggc gttgccgaag ctcggggcct ttccgcagga gtaacagatt
    21061 ttcgccatgt ctcgtctcct atgctaggat agctgtcgaa ttcgggggcc caagtcgcgg
    21121 ctgctcgcgc gccgcggacc cgggcatcgg gcggaaattg tatcagattc ttggagagcg
    21181 acagaacgtg cggagccgga tggtttcgcg gtggactgtg ccgggaggag cgatttggag
    21241 cgcgggttga gaggttcgtt ttcgtggcga taaggatggc cttcgtggtg gccgccgcac
    21301 acgcggctct gaagtctcag gtcgccaggg tcaacgccct gaacgtctac ccggtcccgg
    21361 acggcgatac gggcacgaac atgctgctga cgctcgaatc catcctcaag gaagcgaaag
    21421 acaagaccta tgagagtgcg gaggcggcga gcaaggccgg ggcgcgggcg gcactcatgg
    21481 gagcgcgggg gaactctggt gtgatcctgt cgcagatgat ccggggcgcc tgcgaggtgc
    21541 tggcgaagcg cgcctccctc acggccgaga ccttcgccga cgccctcgaa ggagcgcgcg
    21601 agcgagccta cgcttcgatc cgccagcccg tggagggcac gatgctcacc gtgatcaagg
    21661 acgccgccac cgcggcccgc gaggccgtga gggaccggaa cgtggacctt ccggctgccg
    21721 tcaaggcggc ggcgcaggcg gcccacgcat cggtccggcg cacgccggag ttgctcggcg
    21781 tgctgcgtga ggccggggtc gtggacgccg ggggcttggg cgtggcgata atcctggatg
    21841 gaatctatgc ctgtatcacc gggcagaaga tcgaagtgga cgaagaggac gagtccggga
    21901 cgccggacct tgacgcgatc cacgccgagg aagaagcctg gggctactgc acggagttcc
    21961 tcgtgacggg gttcgagggc gacgccgggg agttcgagga ctacatatac gcttccggca
    22021 agagcgtgct ggtcatcccg gatgacgatg tgatcaaggt ccacctccac acccaggacc
    22081 ccggcagcgc cctgacctac gcgggtactt tcggaaggct ctccggcgta aaggtggacg
    22141 acatggaagc ccaggtccac gcgcgcgggg acggaagcac ggaaagctcg gccgaatcgg
    22201 cgagcatcgg tgtggtggcc gccagccggg gcgccgggaa ccgaaagctg ttcgagtcga
    22261 tgggggcggt cgtgatcgag ggcgggcagg gagcgaaccc gagcgcggcc gacttcgccg
    22321 cggcggtcga caagacgggc gctccagccg tgatcctcct gccgaacaac aagaacatcg
    22381 tcccgaccgc cgagcaggtc ggagagctcg tcgaggcgga gacctacgtg gtaccaacga
    22441 cgaccattgc ctcgggactt gcggcgatgg tcggtttcga cggcgagggc gaggccgatg
    22501 acgtggccga ggagatgtgc gagatcctcg acacgctgcg gagcgcggag gtgacgcggg
    22561 ccgtgaggga cgtccgcatc ggcgaccatg aagtgtcgga gggagcgttc atcgggttgc
    22621 tggacggaga ccttcacgct gtcggagaga gcgtggagga agtcgcgctg aagctcgccg
    22681 aggatatcct ggacgatggg gcggacgtga tcacgctgct cgccggagag gacctggatg
    22741 gggagatcct ctcgcggata tccgaccgga tcaaggccct ggacgaagat ctcacggtcg
    22801 aggcgaagac cggcgggcag ccgatgtacc cgctccagat ggtggccgag tgacgaccgc
    22861 catcgtcacc gactccacga ccagcctcgc gcccgagctg cgcgaacggc ccgatgtccg
    22921 cgtcgtccct ctgacattcc acttcggccc ggacgagacc tacctggata aggtggacat
    22981 gtcgaacgcg gagttctacg agcggctcgc tgattccgac gagttcccga cgacctcgca
    23041 gccaccggcc ggcgctttcg tggaggctta cgaatctcta tcggtatacg acgacgtgct
    23101 agtgctcacg ctttcgggca aggtaagcgg cacctacgaa tcggccgtgg cggcggcgga
    23161 gatggtggac cgtccggtgg aggtgctcga cacgcggagc gcggagatgg gctcggggct
    23221 aatcctgctg gaggccctga aggtgatcga cgagggcggc gagttccggg acgcgcggcg
    23281 ggccgccgag gcggcgatcc ggcgctgcag tatcctgttc gctgttggta ctctgcagta
    23341 tctggcgaag agtgggcgta tcggacgggc gcagcggatg gtggggaccg cgctggacat
    23401 ccggccggtc ctggccctgg aggacggaga ggtcgtccct cacaagcggg cgcggggacg
    23461 caagcgccag atggcggcgc tggtggagca gttacagccg gcggtcgagg cggggtcctc
    23521 gctgtacttc gggcacatag acgcgccgga agctgtatcc gagctggccg ccgccctggg
    23581 cgtagagcag gagagggtcg cggagatcgg gggcgtggtc ggttgccacg tcggtccggg
    23641 agcctatggc gtggcctacc tctagctctg gcgccgcacc gggccagacg cccgcccgtc
    23701 tgaacctccc cgaacgcacg accctgacgg acctgcgggc tcgccccgtc accgggcttc
    23761 cgggcgtggg gccgaggatc gcggcggcgt tgaaggacct gggtatcgag tcgctggcgg
    23821 atctggtatc gcactacccg tcacgtcacg aagacctgtc gaacgtcaag aagatctcgg
    23881 acctccgcgt cggcgagcgc gctacggtga tcggccggat agtgagcaca cggcccgtcg
    23941 ggaagccggt ccggggccgg tcaccagggt tctccgcgca gctctacgac gggacgggat
    24001 acatcccggc gaccgtgtgg ggccggggct ggttgcaggg tcagctcgtc cccgacacct
    24061 gggtgctggt ctcgggcgag gtgcagcggc gctacgggat acagctcgcg gccaagagcc
    24121 tggaggtgct ggatgaccgg gacgcgccgg gcgatggcgt gagcggtccg cacgcgggac
    24181 ggttcgtgcc cgtgtacccc gtcaataagg ggatacagac caggaggatg cggaccctga
    24241 tccaccgcgc gcttgacgag gcgggacgta tcctcgaccc gctgccggcg gaggtcctgg
    24301 tgcgccaccg gctcccgaac cttcacgacg cgatgcacga ggtccacttc ccatcggaga
    24361 agaaacgcct gaagtcagcc atgcggcgtc tcatcttcca cgagctgttc atgatccaga
    24421 cgggcctcgc cgcccggaag gcgcgcatcg aacgcaccga gcgcggcggc cggcatatcg
    24481 gcgacggctc gctactgaac ccgttcctga aaggtctgcc gttcggactg accggggccc
    24541 aggagcgcgt gatcaaggag gtcctcgccg atatgcgctc cgagaagccg atgcgccggc
    24601 tggtccaggg agacgtgggg agcggcaaga ccgtcgtcgc cgtcgcggcc ctgctctcag
    24661 cggtcgagtc cggcggccag ggcgctctca tggccccgac cgaggtgctg gccgagcaac
    24721 actacctctc cgtctcaagc gcgctggccg ggttgccggt ccgggtggcg ctcttgacag
    24781 gctcgcaggg cgcggcggag cggcgggaga tgctggagct ggttcgaagc ggcgaggcaa
    24841 acatcgtcat cgggacgcac gccctgatac agaaaggtgt ggagttccgg gacctctcgc
    24901 tcgtggtggt ggacgagcag caccggttcg gcgtcggcca gcggatggta ttcaaggaga
    24961 agggcgcgat gcccgacatg atggtaatga cggcgacccc cataccgcgg accctctcgc
    25021 tcacgctcta cggcgacctc gaagtctccg tcatagacga gttgccgccg ggccgcaagc
    25081 cggtcgagac ggtgctgatg gacctctcgg gccgcgaaga agcttacgag gccgtgcggc
    25141 gggagctgga ggatggacgg caggcgtatg taatctgccc gctggtagaa gagtccgagg
    25201 cgctggaaga cgttcgggcg gcggaggagc tgtatgacga gctggcggag gagatcttcc
    25261 cggatcgcaa ggtcgggctg ttgcacggcc ggatgaaggc gcaggagaag cgcgaggtca
    25321 tggcggcgtt ccgcgcgggg gagatagagg tgctggtcgc caccgtcgtc gtcgaggtcg
    25381 gggtggacgt gccgaacgcg agcgcgatcg tgatcgaggg cgccgagcgg ttcggcctct
    25441 cgcaactcca ccagctccgc gggcgtgtct gccgcggact ccaccctccg aagtgcttcc
    25501 tcatcggcga gccgaagacg gatgactcgc gaagccgcct cgaagccctg tgcgagcacc
    25561 aggacggatt caagctctcc gaggtggacc tggcgatccg gggcgagggc accctcttcg
    25621 ggagccgcca gagcggtatg ccggacctca aggtcgcaaa gctcctccgg gacatagacg
    25681 tgctggtcga ggcgcggcgc gaggcgttcg agctggtggc gaaggacccg acactgaagg
    25741 cccccgtcca ccggccgctg cggcgtgaga tccgggagct gctcggcgcg gacgtcgagt
    25801 ggctcttccg ggagtaggag cgccgtgcga gtcatagccg gcgaggcgcg gggtgtgcgc
    25861 ctgacgaacg tgccgcccgg agtgcgtccg accgcggacc gcgtccggga gagcctcttc
    25921 aaccgactgg ggcagttctt cgacggcggc gaggtgctcg acctctacgc cgggacgggc
    25981 gctctgggca tcgaggccct cagccgggga tgcgagcggg cggtgttcgt ggagaaggac
    26041 cggcgggccg tggcggcaat ccgggagaac ctgcggagga cggggtttct ggagcgcgcc
    26101 gagatcctgc gcgacgatgt aggtagagcc ctagatgcgc tggaggggcg cggcggacaa
    26161 tacaatttga tattcattga tccgccatat agaatcgcgg cgctggaggt cggcagcgta
    26221 ctcatgcgtc tgcgtgctat tctggcgccc aatggtaggg cggtggtcga gactggcggc
    26281 ctctcgccgg aagttataga gagcaagaaa ggggagagcc ggcgctacgg cggcacggtc
    26341 gtaactatct ttgaacgttc ggacctcacg atgaacgtgg ccgtatgtcc cgggagcttc
    26401 gatcccatca ccgccggcca tctggacata atccggcgcg cggcggggat cttcgaccat
    26461 gtcgtaatcg ccgtgggccg gaacctcaga aaggggccga ggcttaccgc gagcgagcgg
    26521 gcgcatctta tcgagaccgt gacccgcgac atcgacaacg tgtcggtcga gttgatggac
    26581 ggcctgctgg tcgatttcgc gcgggagcag ggcgcgcgcg tgatagtaaa ggggctgcgg
    26641 gcggtctccg atttcgagtc ggagttcgag caggcgcaat tgaatcggaa cctctccccg
    26701 gagatcgaga cggtgtttat catgtcggcg gcggagtaca gtttcctgtc gtcgagcgcg
    26761 gtgcgcgaga tagcgggcct gggcggagac gtgcgcgggc tcgttcccga tgtaatactt
    26821 gatgtggtgc gcgagatata ttcgggcagc ggagagaaga cagagactac acgacaaagg
    26881 ataggataag aggatggacg ttctggtgct tatagacagg ttggaggagc tggtggaaga
    26941 agcccgaagc ttccccggct tcggcaacac ggcgatggtc gaccgcgatg cggcctttga
    27001 tgtcatagac cagatgcgcc agaccatccc ggaggaattg aagcaggcgc gctggatcgt
    27061 caaggagcgc caggcgatgc tggacgaggc gcgtagcgag agcgaccgta tcatccgggg
    27121 cgcgcaggag gaggccgaga agatcacctc cgaggaagaa gtcctcaagc gggccgagaa
    27181 gcgctccacc gagatcatgg aggacgcgag gcgccgggag cgcgagatcc gtctgggcgc
    27241 cgaggattac gccgacgagg tcctcgccaa cctggaagac aacctcgcca agctcctcga
    27301 agccgtccag cggggccgct cgaagctcca gggcggcgag gaagagcagg cgcggtagaa
    27361 taaccccatg agcaattcta tccccctcag gcgtccgcgc gccgaggtcg gagaccggta
    27421 cgagcagcga gcgagcttca ccgttccggc gttcgacctc tacggcaggc atcacgagat
    27481 agagaacgcc gaggccgacg tggccgtgac gcgcctcgcc gaaggtctgc acctggacct
    27541 ggaggtgcgc gttgtactgt caacgacgtg cgaccggacc ctggagccga cggaggtcga
    27601 gctgctgttc ggcgagtccg agttcttgcc cggcccgaac aaccgggagc tgtgtatccg
    27661 ggagtgggag ttggacctcg tggggtatac ggagcgcgct ctgccgagcg aggtgccgat
    27721 gcaggtgttc catcccggca caaccgcggt tcgccccgag gagagcgcgg aggagaccga
    27781 tcctcgctgg cgcggactcg gtgggctgtt cgcctcgggt ttctagattt aatcaaccaa
    27841 tagcaggagg taagtcatgg cagttccgaa gaagaagacg tcgcaggctc gcagagacca
    27901 gcgccgggcg caccacgcgc tgaagagcgc aaacctggtg ccgtgcccga catgcgggta
    27961 tccgagggtg ccgcaccggg tgtgcccgga gtgcggcagc tacaagggcc gcaacgtggt
    28021 cgccgtcgag gccagcgagt aacatccgac cccgcatccc gtgcggatag ccgtagacgc
    28081 cctcggtgga gataacgcgc cgggggagat ctttgccggg accctggccg cggcgcggcg
    28141 cctgcccgaa gacgagttca tcctcgtggg agcgagcggc gggacccaaa cgttcgccgg
    28201 tgcgcgggag gtcccgccga acatctcttt ccgtgaatcc ggcggcgcta tcggaatgga
    28261 agaagagccc gccgccgctc tgcgctcccg gccggacgcg agtgtggcag tcgctgcccg
    28321 gatggtgcgg gcgggggagg ccgaagcgtt cttctccgct ggcagtacgg gggcgacggt
    28381 cgccgctgcg cttctgtcgg tggggcggct caggagttgc cggaggccgg ctatcgcgac
    28441 ggtgctgccg tttccatcgc ccgtactgct tctcgatgcg ggggctacga tctcgtgccg
    28501 ggcgcacgac cttgtgaatt tcgctattct tgggagcgtg ttcgcgaaaa gatatttccg
    28561 gctcggcggc gaggcccgcg ttggtctgat caacgtcggt gaggagccgt cgaaggggac
    28621 cgacctgacg aaagaggcat acgggatgat gcgggactcc ggcgcgatag atttcgtcgg
    28681 caacgtggag ggccgcgaca tcggacgcgg cgtggcggac gtgctggtta cggatggatt
    28741 tacgggtaac gtcgtgctca agacagccga gggagtggcg agcgagatcc tccagatggt
    28801 ccgctccgcc gttacggtcg gtatcgtgag caaggctgcc gcagccgtct tgcgtccgcg
    28861 tctgatcggg gtgcgagacc aggtcgatcc cgagaactac ggcgggtcgt tcctgctcgg
    28921 agtgcgtggc tcggtcgtga tcggccacgg aaactcggaa gcccgcggcg tagagaacgc
    28981 actgctcgga atctcgcgca ccggcgcagg gctcgccgcg gtactcgaag aagccctcgc
    29041 ggcctctcag acaaaggagg cggaacactc gtgagcggag cgccggtcgg gaagatagtg
    29101 ggcgtgggcc gcgcgctagg cgggcgcgtg gtcaccaacc acgacctcga aaatgtcctc
    29161 gatacgaccg acgactgggt gaagacccgc acggggatca gagagcggca cttcgtcgcc
    29221 gagggggaga cctgcgccac gctcgccacg gacgccgctg ttgcggcgct gaagcatgcc
    29281 ggtgttacgg gtgagtcgct ggaccttatc ctttgcgcta cctcgaccgg gccggaatcc
    29341 atgccttccg tggcctgtct cgttggtgag gcagtgggcg cgccgggcgt cggggcgatg
    29401 gatctcgcgg cggcgtgcgc cgggttcagt tacgcggcct ccgtcgccga ttcgatgatg
    29461 cgcggcggca tcgcggaacg ggtgctcctg atcggggcgg acgcaatgac cgccgtcgta
    29521 gatggcaacg accgcgccac ggcggtcctt ttcggtgacg gggcgggcgc ggtagtgctg
    29581 gagcggggtg agggcgagtc cgggtttgtg gatcatatcc tcggtgctga cgggcggatg
    29641 gcttctgccg ggcgagccgg gcatcccggc gacgagcaga agctgtatca gaatgggcgc
    29701 gaggtcttca agttcgcggt tcggatcctg cccgagatgg tagagaagct cgtcgcccgt
    29761 aacggcatca gcctcgacga cgtacagtac atcatcccgc accaggccaa cgcgcggatc
    29821 atccaggcgg cggcgaggaa gctggagata ccggaggagc ggatagtcat aaatgtggac
    29881 cacttcggga acacgtcggc cggcagcatc ccgatctcct acccggacat ttacgacgat
    29941 ttcgagccgg gcaagtacat aatcacggtc gggttcgggt tcgggcttac gtgggcggcg
    30001 aacctttaca ggatctaggg ctttttaggg atggaaaggg gaggggtgca gataaggcga
    30061 gcgattgtct ttccagggca ggggaccgtg ggcggcgagg tctccggcgg gatccaggat
    30121 gctgttcagc ggcaagtcgg tgacggggaa gtcccgtacc aactctccgt cttcgccagc
    30181 tcggttcatc ttctgggccg tctgcgcgag cacggcatta aaccgaacgt cgtggccggg
    30241 cattcgctgg gcgagtacgc cgccgcccat gccgccggaa gtctcggcct cgaggacgga
    30301 gtgcggctcg tggccgagag ggaccggctg atgaacgagg cgtcccgcga gaaccccggc
    30361 gcgatggtcg ccctgatcaa ggcggacccg atggacgtgg tttccgcggt cgaggaggtc
    30421 gagggggtcg tggtcgcggc gaacttcaac acgccgcggc agactgtgat ctccggcgag
    30481 gaggaggcgg tgcaggcggt cgcggattcc gtgaagggcc ggaaggtccg tctcgatgtc
    30541 gcgggggcgt ttcactcacc gctgatgctg gaggcgtcga gggctatgtc gcggttgctg
    30601 ctggaggtcg agttgcgcaa gccggagatc ccgatggtca gcgcggtgga tggcgcgatc
    30661 ctggcgacgg tggatgacct gcggcaggcg ctcacgaacc agatgctctc tccggtcagg
    30721 tgggtcgcgg tgatcgagcg cttccgggac ctgggggtgc gcgagatggt cgaggcgggc
    30781 gagggaggca cgctgacgcg catgctgcgg gacttcccgc aggaccgcct gaagggaatg
    30841 accgcgaagg aggcgctcgc atgaaggcgc cactgggacc ggcggagatc cgggctctca
    30901 tcccgcaccg ttacccgttc ctgctggtgg accggatcga ggagatcgag cccggaattc
    30961 gagccgtcgg cataaagaac gttacccaga acgagccgtt cttccaggga catttccccg
    31021 actacccggt gatgccggga gtcctgatcg tggaggcgat ggcgcaggtc ggcgccgttg
    31081 gggttatggc ggtagaggag taccgccaga agctggcgct cttcgccggg atagatggcg
    31141 taaggttccg gcggcaggtg gttccgggag acgtgctgcg gatggaggtc gagatctcac
    31201 gcctcaaggg aagcgtcgga cgcggcaagg ggcgggctac ggttaacggg gagcgggtct
    31261 gcgaggcgga cctgatgttc gccttcgctg cgcgagggga ggtcgagtga tcgagtccgg
    31321 cagggtggca gtagtgaccg gaggaggccg cgggatagga cgggcgattg ccgtccggct
    31381 ggccgaggag ggcgcgaacg tcgccatctc ctaccgctcg aacgactccg ctgccgagga
    31441 gaccgccgcc gcagtccgcg aggcgggcgt cgagtgcgag ctgttcaagg gagatgtggc
    31501 ggagccggat gacgtggccg cgctgttcaa gggcgcgcag gagaaatttg ggcgcgtgga
    31561 catcctggta aacaacgcgg gcacgacgcg ggacaacatc atgctgcgga tgaaagaaga
    31621 agagttcgac gaggtgctgc ggacgaacct caaggggacg tacctctgca cgcgggcggt
    31681 cttgcggacg atggtgaggg cccggtgggg tcggatcgtc aacgtcagct ccgtggtggg
    31741 gctggttggg aacgcgggac aggcgaacta cgcggcgtcc aaggcgggca tcatcgggtt
    31801 tacgaagagc gtggcgcggg aggtggcgca gcggggtatc acggtcaacg cggtggctcc
    31861 aggctacgta gagacggagc taacgggcaa gttgccggat acggtgaaag accagatcaa
    31921 ggcccaggtt cctaccggga ggttcgggga gccggaggag gtcgccgagg tggtcgcgtt
    31981 cctagctggc gagagggccg gatacgtaac cgggcagacg cttgccgtgg atggcgggat
    32041 gacaatgcaa tagacgtgcg agggatggat ttgcgctaca ctacgcgcta tcttcaccgg
    32101 gagatggtga accttgttgg gagatggaag aatcgcaaag gagagacaga gtgagccgag
    32161 acgagatact ggcgaagata caggagatta ccgccgaccg gctgggtgtg gacgagggcg
    32221 acgtcacccc cgacgcctcc ttccgcgagg acctggaggc cgactccctg gacctcgttg
    32281 agctgatcat ggagctagag gagcagttcg ggatggagat cccggacgac gacgccgaga
    32341 agatcacgac ggtcgaagag gcggttgact acgtgtcgga gcaccaggcc gcgtgacgca
    32401 gggcgcttca aatggcaaat gggccggtaa cggccacgcg ctcatcaccg gcacgggggc
    32461 cgtcacgccc ctgggcaccg ggaccgagaa cttctgggac gcggcgctcc acggcaagag
    32521 cggtgtcggg gagataaccc tcttcgattc ttcgccttat ccctcacaca tagctggcga
    32581 gtgctcggac ttcgatccaa cggacttcat gagcaagaag gaagcccgga ggatggaccg
    32641 cttcgcccag ttcgggatag cggccgggat acaggccgcc gagagcgctg gtatcgtgga
    32701 cgtcatccgg gagagacccg agcgcgccgc catcgtgatg gggaccggca tcggtgggct
    32761 ctcgacctgg gagcgggagt acaaggtcct gaacgagcgc ggcgccggcc gggtgagccc
    32821 gttcctgatc acgatgatgg tcccgaacat ggccgccgga cagctcgcca ttatgctcgg
    32881 catcacgggt ccgtcgcagt gtcccgtcct ggcctgcgcc acgggcggcg aggcaatcgc
    32941 ggaaggcttg gagttgatcc gccgcggcga cgccgacgtg gtgatcgcag gctcctcgga
    33001 agcgccgatc acacccctca gcatggccgg cttctgcgct atccgggcga tctcgacgcg
    33061 caacgatgag ccgcagcggg cgagccggcc gttcgacgcc ggacgcgatg gcttcgtgat
    33121 gagcgagggc gccggcgcgg tcgtgctaga gagcccggag cacgcggccc ggcgcggagc
    33181 ggagccaata gccgccgtgg cgggctacgg acgctcgact gacgcctacc acgtcaccgc
    33241 ccccgacgag cagggccggg gcgtggagcg ggctatgcac gccgctctca aggactccgg
    33301 tctgacggcc gaggacatcg ggcacgtcaa cgcgcacgcc acctcgaccc cgaccggcga
    33361 cgggccggag acgaaggcca tctcgcgggt catgccgcac gcgctggtct ccggcaccaa
    33421 gtccatgacg ggccattcat tcggagcggt cggggcgacg gaggggatca tgtgcgcgct
    33481 ggcgatcaag aacaagatag tgccgccgac gctgaatctg gacgatctgg ccgaggactg
    33541 cgagccgctc aactacgtgg gttccgaggc acagaaggcg ccggacctcc gagccgccat
    33601 ctcgaactcg atggggtttg gcggtcataa tgtcgtcgta gctttcaggg atgtagaata
    33661 gttcgatacg agatgtccat aaaggatctc atagcgattc ttccagagcc gctgaggcag
    33721 cgttcgctca cgcaccccag cgtcgcggac ccctacgact ctaacggccg gctggagttc
    33781 ctgggggacg ctatcctgaa cacccgcgtg gcggagctag tcttccacag ctacccggag
    33841 cttcttgagg gcgacctaac tcgcatccgg gctcatctgg tccgcaagac gttcctggct
    33901 acggtcggac gaggcgaggg gctggaggag atcatcgaga gctcggtcat caacgactcg
    33961 gtcatcgctg acaccgtgga ggcgatactg ggagccgctc acctcataga ccgcgacctg
    34021 gtcgcccgga cgatagatga gatcttcaat ccttccgagg tagatacgga cgagcttcgt
    34081 gactggaaga cgctcctgca ggagacgctc caggcggatg ggttgcgtcc tacctaccac
    34141 gtcctgtcca aacagggacc gccgcaccgt ccgaacttcg tctccgaggt ctcggtggac
    34201 ggcaggaacg tcgggaccgg cgaggggcac tccatcaagg agagcgagca agccgccgcc
    34261 cgggcggcgc tggagatcct gggcatccgc ccgttgccgc gctcggcggt cgaggtacat
    34321 gtaggcgcgt agcgcctgct ctactcttct ggcagctaat ttgctgcact ttcgcgtgct
    34381 ttgcgagtga attgtgcgta aaatgcgtcc gtagtgctct cggcggtcta catcaagggg
    34441 ttcaagactt tcgcccgccc ggtacggatg cccctcgaag gcggcctgac cgccatagta
    34501 ggcccgaacg ggtccggcaa gagcaacatc acggacgcgg tcctcttcgc cttcggggag
    34561 cagagtcctg gggtgttgcg ggcgggggtg atgggagatt tgatcttctc gggttccgag
    34621 acgcttccgg cctcaggtgt cgccgaggtg acgcttgtcc tggacaatga atccggcgag
    34681 atctcgctgc cgtaccagga agtgtcgatc tcccgccgca tctcccgtaa cggcgacact
    34741 gagtaccgtg ttaacggctc tcgcgcccgg ctcgccgacg tgcgggccgt cgccggagaa
    34801 gccggactcg gccgccattc catcctgcgc cagggcgcgg tcgacgcgat agtggcgggc
    34861 ggtgccgccg cctgtcgtct ggcgctggag gaagcggccg gactgggggt gtaccggcgc
    34921 cggcgtctct ctgccagccg ccgtctggag aaggcccagg cccaactgga ccagagccgg
    34981 caactggagg ccgagctcgc ctcccagttg cggcgcatcg agcgggaagc cgtggccgcg
    35041 cgggagtacc gcgaggtcga ggggcgctac cgcaagctct cactggccca cctctaccgc
    35101 gttgcggatc agggcctcga tgaacgccgg aagcaactcg aacgctccga gaagtcggtc
    35161 gtggctctgt cggagcagga gcgggcgttg cgcgaggagg aggctggcat ctccccgcag
    35221 acgcagcgcc ttgagagcgc catgcggagc ctggagcgcc aggccgaaga gctagaggag
    35281 gcggaggagg acctccgcgc cgagacgatg cgcgctgacc gggcgctcct gcggctggag
    35341 tcgagagccg gaggaacagg cgaccggcag cggaacattg ccacgctgga ggccgaactg
    35401 gagcgtatag agcgcgcgct gggggcgatg cggcgggagt tggatctcct ggagatagaa
    35461 cacacccgta agcgcgagaa gcagaccgag ctgcggcgga tgctggatga gcgccggcag
    35521 cgccaggggg aggccgacag gtccaggtcc aggatcgccg ccgagctgga gggccttcgc
    35581 ggacgccgcg agcgtatcct cgcccgcggc acgaagtccg ggaacgtggg ggaggagtat
    35641 cttcgggaac tcgccgaagt ctcgggcgag atcgagcgac tgaccgagac tggcttcgcg
    35701 gctcccggac gtaatattcg agatacgtta ggcgggttac gcgccgccct ggatgagcgg
    35761 gcggccggga tcaaccgccg cggcggcgcc ctggaggccg ctcttggcgc tgcggaagcc
    35821 gtgattcgct cactgagaga tgccgacacg caggacgctg gcggtcccag gttgcacgag
    35881 gtgatccgcg cccggcccgg cttcgaaggc gcggtggagg ccgctcttgg ggatgcggcg
    35941 ggcgggctcc tggccgcgaa cgtgagcgag ggcatcaagc tgctctccga gggcgaaccg
    36001 gtcgccttgc gtctggacgc tcgcccggta gaccactatg cggatcatcc cgggctgccg
    36061 ctgctcgatt gtgtggaact ggtcgacgag gagtatgcgg aggctgtcga gcgtctcctg
    36121 gccggtatct acgtcgtaga ggacccagcg catgaccacc cggagaacgg tcatgtagtg
    36181 gtcacccgca acggcttccg tctcacccga acgagcgtgc gcctgcgttc tgaggacgga
    36241 cgcttctccc ggaaggcgcg gctggaccgg gagcaaaatc gcttgcgg
//
LOCUS       DASSMB010000198         8893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_938249, whole genome shotgun sequence.
ACCESSION   DASSMB010000198 DASSMB010000000
VERSION     DASSMB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938249"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFJ72_12110"
     CDS             complement(<1..228)
                     /locus_tag="VFJ72_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7480249.1"
                     /translation="MPKLWNETIEAHRRAVHDAILDATAVLVAEHGLLSVTMSRIAEE
                     TGIGRATLYKYFPDVEAVLLAWHERHVTGHLE"
     gene            353..541
                     /locus_tag="VFJ72_12115"
     CDS             353..541
                     /locus_tag="VFJ72_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480250.1"
                     /translation="MADPSSDTGTPRWVKVFGIIVIVVVLLFVVLQFTGVDGHGPGRH
                     IGIHTLPSSVTERGVQQS"
     gene            538..1059
                     /locus_tag="VFJ72_12120"
     CDS             538..1059
                     /locus_tag="VFJ72_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480251.1"
                     /translation="MTMTPRLRKLALTAHITSSVGWLGAVAAFLGLAIVGLTSQDAQT
                     VRGAYLVMEPAAWFVLVPLAFASLLTGLVQSLGTKWGLFRHYWVLFKLLITIFATIVL
                     LIYMETLSLMAGLAADPNTALAMVRDDSPLVHTVLALLVLLVATVLAVYKPRGMTRYG
                     QRKQHERRTESRP"
     gene            complement(1170..2309)
                     /locus_tag="VFJ72_12125"
     CDS             complement(1170..2309)
                     /locus_tag="VFJ72_12125"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7480252.1"
                     /translation="MAALLVFIGMSGLGSGESVGGPAPDEVTITEAAPEDGATSSDSG
                     SGTSVREVYTRDGPGVVSVDVASSEMGPGGGSGFVLDKNGYIVTNQHVVSGADGVSVR
                     FASGARRDAEIVGEDPSTDVAILKVDAPKDTLEPLTLGDSESVTVGDPVIAIGNPLNV
                     GLSVTTGIVSGTGRPIKAPNNYTINDAVQTDAAISSGNSGGPLLDARGAVIGINSQVA
                     SAGSNGVAQGVGFAVPINTVKGVVEQLITNGEVVHGYIGVRMFQIGVEELRAYTTLSE
                     QEFRDKYGLPKNGAIVSEVVPNGPADDAGISGGKGDEKEIAGGSVPLGDVITTVDGKR
                     VSDPDDVIEVVNSKKPGDMMDLIVVKPGGEPRNVRVKLGTQPEGA"
     gene            complement(2413..3903)
                     /locus_tag="VFJ72_12130"
     CDS             complement(2413..3903)
                     /locus_tag="VFJ72_12130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET7480253.1"
                     /translation="MLEREIGSGGMARVFLGRDEVLDRAVAVKILRHGYGDSDIGARF
                     RREGRTAAGLSHPNIVQVYDAGEAEFEGKESSYIVMEYLSGGDLKDLIDEKGSMRTGD
                     LAKLGAQVAAGLAHAHERGVIHRDIKPHNILLDEHGRPKLTDFGIARALDATTTSHPT
                     RTGAYLGTALYSSPEQLQGEKVTPKSDVYSLGATLYQAATGEPPFSGSALSVASQHVS
                     KPPPPPRELNGSIGAPMEALILECLAKDPDERPSAEEVQSRLAGIDSEAEDTRLYAAP
                     LPEQPTRQNNQEEATQAAPVRDISRNRLRTLLAILALLVILALVAAIALPALLGGNGG
                     NGQSADKPNGGKPSNSAKSSGSSGNNSPQATTPAKQETTQQPSPPKSEKTTEPTQPKG
                     LTAQAAEQTIRDHYAVAASDNYQDAWNFLSTGYQQRLGSRESWTSQFQDLESVEFTDG
                     PTAKVNGDTVTVTFSTIARHTNRTDRPSLTATLVDQNGEWKIDSLN"
     gene            complement(4002..4814)
                     /gene="aroF"
                     /locus_tag="VFJ72_12135"
     CDS             complement(4002..4814)
                     /gene="aroF"
                     /locus_tag="VFJ72_12135"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7480254.1"
                     /translation="MDVSTDVKTHVKTTNGMIQPSSFRVIAGPCTVESYDQFVASATA
                     VKAAGFEYVRGGAFKPRTSPYSFQGLGEEGLKILSEVSGGLGLKVVTEVMDPYDIEMI
                     MEHAHILQIGARNMQNFSLLKRVGAAIAGTDHGVVLKRGLAASVEEWILAAEYITAAG
                     GDNVVLCERGIRTFETSTRFTLDLSAVIVAKRLTDLPVIVDPSHAAGRRDLVVPLSKA
                     SVAAEADGLMVESHHDPQEALCDAEQALPVEALMGLKDVLQPFASAMGREVI"
     gene            complement(4817..5098)
                     /locus_tag="VFJ72_12140"
     CDS             complement(4817..5098)
                     /locus_tag="VFJ72_12140"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET7480255.1"
                     /translation="MRDRVAALRERVDAVDRDLIRALNERARIVQEITTIKAEAGIPV
                     FDPKREEEILRRAVEYNEGPIYDSSMREIFEIILHRIRDLEIQRGEFSK"
     gene            5299..6363
                     /locus_tag="VFJ72_12145"
     CDS             5299..6363
                     /locus_tag="VFJ72_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7480256.1"
                     /translation="MKFRPELDPLRPYVPPKTWRMSAEGTDENRYAKLTSNELAFGPL
                     PEAEAALSEVLPRANRYPDSHVSRLRQVIADANPGTETRNVLVGNGSSEVLLNSLQLL
                     PGGGEIVYPWPSFSLYPMVCAVLGMTARPVPLTKENEIPAEALLSAVGDRTRAVILCN
                     PNNPSGTYLTLEEVHNFADALPQEVLLILDEAYVEFVDDPAFVDSHTLGLERENVVVA
                     RTFSKAHGLAGLRCGYGIASGRIADYAERVRFPVSVNLAAQVAATASMLAREKVEKRA
                     EFVIRERRRLQEAFESANLDFIPSQGNFVMVRFEAEEFEKSGVLVREGAALGYPGWSR
                     VTVGNSEENDRVIASLGALG"
     gene            6367..7413
                     /locus_tag="VFJ72_12150"
     CDS             6367..7413
                     /locus_tag="VFJ72_12150"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET7480257.1"
                     /translation="MARSLGIVGVGLIGGSVGLAARNAGWEVVGVDAPGVLEKAVENG
                     AIHRASTIKEVRGADLVVLAAPISKVTGLVADLAPTDTLVTDVASAKSAIVRAAEEQK
                     LRFVGGHPMAGSQLAGVANAREELFKGARYFLTPTERTDPDDYREVATFVRDLGAVPT
                     AVDPEKHDLLMAALSHLPHLMAAALLKVASDISPEALSFAGPSFRDLTRVGASNPELW
                     SDILAENAPALGEALGAFAGAMAQLGSEIKDREVMEGRFRQAREAYDALGGILVEKSG
                     ENMDVAIPVENRPGVFAEVTTLMGSNSINILDLYVRHSNTERAALVLTLDAGDAVRAR
                     ELLRRAGFGAEIQS"
     gene            complement(7448..8458)
                     /locus_tag="VFJ72_12155"
     CDS             complement(7448..8458)
                     /locus_tag="VFJ72_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480258.1"
                     /translation="MAGNTVTAGRRGTLPGLYALLFFIFSGALAACGGETEQGSGQGG
                     TTTAQASGDTWSYELPGEEVYPEGVVYDRSSGDYFVGSTSNGEIFRGSVKEGGGSADV
                     FLEGGRDDRSSAIGLELNEDGSRLFVSGGDTGGMFVYDTGSGDLVDSFQNGQKKTFIN
                     DVAVVPSGDAYFTDSFSPVLYRLAATGDGYEFEEYLDLTETPIKYQDGFNLNGIVATG
                     DGRYLIVTHSGTGQLFRIDTRNKKVTEIDLGGDKVAGDGMVLDGRTLYAVAGGGILPV
                     QLSKDFTNGEVGEGFSGSSFKSPTTIAEYNDRMLVVNSQFDARESGNPDLPFTISDIP
                     IP"
     gene            complement(8582..>8893)
                     /locus_tag="VFJ72_12160"
     CDS             complement(8582..>8893)
                     /locus_tag="VFJ72_12160"
                     /inference="COORDINATES: protein motif:HMM:NF019938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HET7480259.1"
                     /translation="WVALDDVAGSIVHSLTHESVSGPVNVGSPNPMTNAEYTKVLGRV
                     LGRPTVLPLPAPAARLMLGEVADALLLSSQRMEPAKLKATGYDFRYPELEGALRHLLG
                     R"
BASE COUNT         1466 a         2970 c         2797 g         1660 t
ORIGIN      
        1 ttctaagtgg ccggtgacgt gtcgttcgtg ccaggcgagc aggaccgctt cgacgtccgg
       61 gaagtacttg tacagcgtcg cacgtccgat gccggtctcc tcggcgatcc gcgacatcgt
      121 caccgacagc agcccgtgct cggccaccag caccgcagta gcgtccagga tcgcgtcatg
      181 caccgcgcgg cgatgcgcct caatcgtctc gttccatagc ttcggcatca caccagtata
      241 cacgacggcg atatcgagac attatgattt gacaataaca tattgtctcg ataaactgct
      301 gccatcgtga atgaaagcac tgacgaccac gcgcgacaga gaggagacgc acatggctga
      361 cccgtcctcc gacaccggca caccacgctg ggtgaaggtg tttgggatca tcgtgatcgt
      421 tgtggtcctg ctgtttgtcg tcttgcaatt caccggcgta gatggccacg gccctggtcg
      481 ccacataggc atccatacgc taccctccag tgtcacggaa cgcggcgtgc aacagtcatg
      541 accatgacac cccgcctccg caagttagcg ctcaccgcgc atatcacatc ctcggtcggc
      601 tggctcggcg cggtcgctgc tttcctcggt ctcgccatcg tcggcctgac cagccaggat
      661 gctcagacgg tgcgtggcgc ctatctcgtg atggagccgg ccgcatggtt tgtcctcgtc
      721 ccgttggctt tcgcttcgct gctcaccggg cttgttcagt cgctgggcac caagtggggc
      781 ttgttccgtc actattgggt cctgttcaaa ctcctgataa ctatcttcgc caccatcgtc
      841 ttgctgattt acatggagac gcttagcttg atggcaggcc tggcggcgga tccaaatacc
      901 gctctcgcca tggtgcggga cgattcccct ctcgtccaca ccgtgctcgc cctgctggtg
      961 ttgctcgtcg ctacggtgct ggcagtgtat aagccgcggg gcatgacccg gtacgggcag
     1021 cgtaagcaac acgaacggcg tacggagtcg cggccgtaga tgcagcgatc cggccctgac
     1081 ctttgctttc ctggggtacg tcttagagta ggcaaggtct gaaagagacc gcaagagtat
     1141 ctgtcaagcc tgggatgagc ggcttcgccc taagctccct ccggctgggt gccgagcttc
     1201 acccggacgt tccggggctc tccgccgggc ttcactacga tcagatccat catgtcgccc
     1261 ggctttttgg agttaacgac ttctatgaca tcgtccgggt cggagacgcg cttcccgtcg
     1321 accgtcgtga tcacatcccc aagaggcacg gagccgccgg cgatctcctt ctcgtcgccc
     1381 tttccgccgg atatgccggc gtcgtccgcc gggccgttcg gcacgacctc gctaactatc
     1441 gccccgttct tcggcagccc gtacttgtcg cggaactctt gctcggagag ggtcgtgtag
     1501 gcccgaagct cctcgacgcc gatctggaac atccgaaccc cgatgtatcc gtggacgacc
     1561 tcgccgttgg tgatgagttg ctcaaccacg cccttgacgg tgttgatcgg gaccgcgaac
     1621 cccacgccct gcgcgacgcc gttgctaccc gccgaggcga cctgcgagtt gatcccgatg
     1681 acggcgcccc gcgcgtcgag cagcggcccg ccggagttgc cggagcttat ggccgcgtcc
     1741 gtctgcacgg cgtcgtttat ggtgtagttg ttcggagcct ttattggccg ccccgtgccg
     1801 ctcacgatgc ccgtcgtgac gctcagcccc acgttgagcg ggttgccgat ggcgatcacc
     1861 gggtccccca ccgtaacgct ctcggagtcg ccgagcgtga ggggctccag cgtgtctttc
     1921 ggcgcgtcca cctttagaat cgccacgtcg gtcgaggggt cctccccgac gatctccgcg
     1981 tccctgcgcg cgccgctcgc gaaccggacg gagacgccgt cggccccgct cacgacatgc
     2041 tggttggtca cgatgtagcc gttcttatcc aggacgaacc ccgaaccgcc tcccggaccc
     2101 atctcgctgg aggccacgtc cacgctcacc acgccgggac cgtcccgcgt gtacacctcc
     2161 cgcacggaag tcccggaccc ggagtccgag gacgttgccc catcttccgg cgccgcctcg
     2221 gtgatcgtca cctcatccgg cgccggcccg ccgaccgact ccccgctccc caagcccgac
     2281 attccgataa ataccagaag cgcggccacc agacctccga caatggcgga gaagagcgcc
     2341 gtcagtatgg tcttcaggag aagttccttc cgtagcttga tcccattata ttcgccgctc
     2401 tgggcggcgc gcctagttca ggctgtctat cttccactcg ccgttctggt ctactagcgt
     2461 ggccgtgaga cttggccggt cggtccggtt cgtatgccgc gcgatggtgc tgaaggtgac
     2521 cgtgactgta tcgccgttaa ccttcgccgt cgggccatcc gtgaactcga cactctccag
     2581 gtcctgaaac tggctcgtcc acgactcccg ggaacccagt ctctgctggt aacccgtcga
     2641 gaggaagttc caggcgtcct gatagttgtc gctggcggcg accgcgtagt gatctcggat
     2701 cgtctgctcc gccgcctgcg ccgtgagtcc cttcggctgc gtcggctccg tggttttctc
     2761 cgatttcggc ggggacggct gctgcgtggt ctcctgcttc gccggcgtcg tggcctgcgg
     2821 ggagttgttc cccgagctgc cggacgattt ggcggagttg gaaggtttgc cgccgttcgg
     2881 cttatccgcg gactgcccgt tcccgccgtt gccacccaat agcgccggga gagctatggc
     2941 ggcgacgagg gcgaggatga ccagaagagc gaggattgcg aggagcgtcc gcagcctatt
     3001 ccggctgata tctcgtaccg gcgcggcctg cgtcgcctcc tcctggttgt tctggcgggt
     3061 tggttgctcc ggcagtggcg cggcatagag gcgagtgtct tcggcctcag aatctatccc
     3121 cgcgagccgg gattggacct cttccgccga gggccgctcg tccgggtcct tagcgaggca
     3181 ctccaggatc agggcctcca tcggcgcgcc gatggagccg tttagttctc tgggtggcgg
     3241 cggcggcttg gagacgtgct ggctcgcgac ggagagggcg cttccggaga acggcggctc
     3301 tccggtcgcc gcctggtaca gggtggcccc cagagagtac acgtcgctct tcggtgtcac
     3361 cttctctccc tggagttgtt ccggggagga gtacagcgcc gtccccaggt aggcgcccgt
     3421 cctggtggga tggctcgtcg tggtggcgtc aagtgcgcgg gcgatgccga agtccgtcag
     3481 cttggggcgg ccgtgctcgt ccagcaagat attgtgcggc tttatgtcgc ggtggatcac
     3541 gccgcgctcg tgggcatgcg cgagcccggc ggcgacctgc gccccgagct tcgccaggtc
     3601 cccggtcctc atcgagcctt tctcgtcgat caggtccttc aagtctccgc cggagaggta
     3661 ctccatgacg atataggaag actctttccc ttcgaattcg gcctcgccgg cgtcgtagac
     3721 ctgcacgata ttgggatgag agagcccggc tgcggtgcgc ccctcgcgcc ggaagcgggc
     3781 gccgatgtcc gagtcgccgt aaccgtgacg caggatcttg accgccacgg cgcggtcgag
     3841 cacctcgtcg cgccccagga agacacgcgc catgccaccg gagccgatct cccgctccaa
     3901 cataaatctc tgcgccagac gtgtcacgca ggtgattata gaggtttttg gttcgctatc
     3961 ggagaagccg gcagacgcgg ccctgacagg cccgagcttc cctagatgac ttcccggccc
     4021 atcgcgctcg cgaacggctg caggacgtcc ttcagcccca tcagcgcctc gaccggcagg
     4081 gcctgctccg cgtcgcagag cgcctcctgc gggtcatggt gcgactcgac catcagcccg
     4141 tcggcctccg cggcgacgct cgccttgctg agagggacga ccaggtcgcg ccgtccggcg
     4201 gcgtgggacg ggtctactat caccggcagg tccgtcagac gcttggcgac gatgaccgcc
     4261 gagagatcca gcgtgaagcg cgtggaagtc tcgaacgtgc ggatgccgcg ctcgcagagg
     4321 accacgttgt ccccgcccgc ggcggtgatg tactcggcgg ccaggatcca ctcctccacg
     4381 ctcgccgcca gcccgcgctt gaggaccacg ccatggtccg tcccggcgat agccgcgccg
     4441 acccgcttca gcagggagaa attctgcatg ttgcgagccc cgatctgaag gatatgggcg
     4501 tgctccatga tcatctcgat gtcgtagggg tccatgacct ccgtgacgac cttcagaccc
     4561 aacccgccgc tgacctccga gaggatcttc agtccctcct cgccgagacc ctggaacgag
     4621 taaggggagg tgcggggctt gaaggcgccg ccgcggacgt actcgaatcc cgccgctttc
     4681 acggctgtcg cagaggcgac gaactggtcg tagctctcga ccgtgcaggg accggcgatc
     4741 acccggaaag aggagggttg gatcatgccg ttggtggtct ttacgtgggt ctttacgtcc
     4801 gttgaaacgt ccatcgctat ttggaaaact ccccgcgttg aatctctaaa tcacggatgc
     4861 ggtgcaggat gatctcgaag atctcgcgca tcgaggagtc gtagatcggc ccctcgttat
     4921 actcgacggc ccggcgcagg atctcctctt cgcgcttggg gtcgaagacc ggaatcccgg
     4981 cctccgcctt tatcgtggtg atctcctgca ctatgcgcgc ccgctcgttg agagcccgga
     5041 tgaggtcacg atccaccgcg tccacccgct cccgtagagc cgctaccctg tctcgcatct
     5101 ccgcgtctgc cataagacct gggattgtat atgccacgtg aatataaggc aaaccctgag
     5161 actatcggga ttcggattcg agccacgagc aagagatcgc caaaacccgt tgcaagctat
     5221 ccctccgggg tctagtctat tgaagccgct cggctggagc cgcccggcta cgccgaaaag
     5281 atcccaagga gaagctgctt gaagttcaga ccggagcttg acccgctgag accatacgta
     5341 ccgccgaaga cctggaggat gtcggccgag ggcacggacg agaaccgcta cgcgaagctg
     5401 acctcgaacg agctggcctt cggcccgctc ccggaggcgg aggcggcgct ctccgaggtg
     5461 ctgccccgcg ccaaccgcta cccggacagc cacgtctcaa ggctccggca ggtcatagcg
     5521 gacgccaacc ccggcaccga gacgcgcaac gtcctcgtcg gcaacggctc cagtgaggtc
     5581 ctcttgaact cgctccagtt gctgccggga ggcggcgaga tcgtctaccc ctggccctcg
     5641 ttcagcctct acccgatggt ctgcgccgta ctcggcatga cagcccgccc ggtcccgctg
     5701 acgaaagaga atgagatccc cgccgaagcg ctgctctccg ccgtgggcga ccggacgcgg
     5761 gccgtgatcc tctgcaaccc caacaatccc agcggcacgt acctcacgct ggaggaggtc
     5821 cacaacttcg ccgacgccct gccgcaagag gtgctcctga tcctggacga agcctacgtg
     5881 gagttcgtgg acgacccggc ctttgtggac tcccataccc tgggtctcga aagggagaac
     5941 gtcgtcgtcg cccgcacctt ctcgaaagcg cacgggctgg cgggtctccg gtgcggctac
     6001 ggcatcgcgt ccgggaggat cgccgactac gccgaacggg tccgcttccc cgtctccgtg
     6061 aacctcgccg cccaggtcgc ggccacggcc tcgatgctcg cgcgggagaa agtcgagaag
     6121 cgggccgagt tcgtgatccg ggaacgccga cggctgcaag aggcattcga gagcgcgaac
     6181 ctggacttca tcccctcgca gggcaacttc gtgatggtcc ggttcgaggc ggaggagttc
     6241 gagaagtccg gggtgctggt gcgggagggt gcggccctgg ggtatccggg atggagccgg
     6301 gtaacggtcg gcaactccga ggagaacgac cgggtaatag cttcgctcgg ggctctcggc
     6361 taggggatgg ctcgctcgct aggaatcgtc ggcgtcggac tgatcggggg ttccgtcggc
     6421 ctcgccgccc ggaacgcggg ctgggaagtc gtcggcgtgg acgcccctgg ggtactggag
     6481 aaagccgtgg agaacggcgc catacaccgc gcctccacga taaaggaagt ccgcggcgcg
     6541 gacctcgtcg tgctcgccgc ccccatctcg aaggtgacgg ggctcgtggc ggacctggcc
     6601 cccaccgaca ccctcgtcac cgacgtcgcg agcgccaaga gcgccatcgt gcgcgcggcg
     6661 gaggaacaga agctgcgttt cgtcggcggg cacccgatgg ccgggagcca gctcgccggg
     6721 gtcgcaaacg cccgcgaaga gttgttcaag ggcgcccgct acttcctgac ccccacggag
     6781 cgcacagatc ccgacgacta ccgcgaggtc gccaccttcg tgcgggacct cggcgccgtc
     6841 ccgacggccg tggaccccga gaagcacgac ctgctgatgg ccgccctctc gcacctgccg
     6901 cacctgatgg cggcggcgct cctgaaagtc gcctcggaca tctcgcccga ggcgctctcc
     6961 ttcgccggtc cctcgttccg ggacctcacg cgggtcgggg cgtcgaaccc ggagctatgg
     7021 tcggacatcc tggcggagaa cgcgccggct ctgggcgagg cgctcggtgc cttcgcggga
     7081 gcgatggcgc agcttggaag cgagataaaa gaccgcgagg ttatggaggg ccgcttccgg
     7141 caggcccgcg aagcctatga cgccctcggc ggaatcctcg tcgagaagag cggcgagaac
     7201 atggacgtgg ctatccccgt cgagaaccgt cccggcgtct tcgccgaggt aacgaccctc
     7261 atgggctcga acagcatcaa catcctcgac ctctacgtcc gccactcgaa caccgaacgc
     7321 gccgccctcg tcctgacgct cgacgccgga gacgcggtca gggcgcggga gcttctgcgt
     7381 agggcgggtt tcggggccga gatccagagc tagaccggtc cggtctcgcg gcgatccgtg
     7441 cgaccgccta cggtatcggg atgtcggaga tggtgaacgg caggtccgga tttccggact
     7501 cgcgcgcgtc gaactgcgag ttgaccacga gcatccggtc gttgtactcg gctatcgtgg
     7561 taggagattt gaaggaggag cccgagaacc cttctccgac ctcgccgttc gtaaagtcct
     7621 tcgagagctg gacgggtagg atcccacccc ccgccacggc gtagagcgtg cggccgtcga
     7681 gcaccatgcc gtcaccggcg actttgtctc cgcccaggtc aatctccgtc accttcttgt
     7741 tcctcgtgtc tatgcggaag agttgccccg tgccggagtg ggtgacgatc aggtagcgtc
     7801 cgtctccggt tgcgacgatg ccgtttaggt tgaatccgtc ctggtacttg atgggcgtct
     7861 ccgtcaggtc caggtattcc tcgaactcgt acccgtctcc tgtcgccgcg agccggtaga
     7921 ggaccgggct gaaggagtcg gtgaagtacg catcccccga cgggacgacc gccacgtcgt
     7981 ttatgaatgt ctttttctgc ccgttttgga agctgtccac gaggtcgccg cttcccgtat
     8041 cgtagacgaa catcccgccc gtatcgccgc cggagacgaa cagccggctc ccatcctcgt
     8101 tcagttccag gccgatggcg gagcttcggt cgtcccggcc gccttcgaga aagacatcgg
     8161 cgctaccgcc gccttccttc acggaacccc ggaagatctc accattcgac gtgctgccga
     8221 cgaagtagtc gccgctggat cgatcgtaga cgaccccttc cgggtagacc tcctcacccg
     8281 gaagctcgta gctccaggta tcgcccgaag cctgagcagt ggtcgtaccg ccctgtcccg
     8341 aaccctgttc cgtctcgccc ccgcaggccg cgagcgcgcc ggaaaagatg aaaaacagaa
     8401 gggcgtacag tccgggaagc gttccccggc ggcctgccgt aacggtgtta cctgccatct
     8461 cgtcctcctg gagtagcctg tgttgccgcc aaacaacacc acactcgata tatacctcga
     8521 cctaccggaa gtcgttcgtg gccgggagga aaagctgtgc gtaaaggatg cggatcgacc
     8581 tctacctgcc gagcaggtgc cgaagcgctc cttccagctc cgggtagcgg aagtcgtagc
     8641 cggtcgcctt caacttcgcg ggctccatgc gctggctcga caggagcagc gcgtccgcaa
     8701 cttcaccgag catgagccgc gctgcgggcg ccgggagcgg caagacggtc ggacgcccga
     8761 gcacccggcc cagtaccttc gtgtactccg cgttggtcat cgggttagga gagccgacgt
     8821 tgacggggcc gctcacgctc tcgtgcgtta gggagtggac gatggagccg gccacgtcgt
     8881 cgagagcgac cca
//
LOCUS       DASSMB010000199         5789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1059957, whole genome shotgun sequence.
ACCESSION   DASSMB010000199 DASSMB010000000
VERSION     DASSMB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059957"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..430
                     /locus_tag="VFJ72_12165"
     CDS             209..430
                     /locus_tag="VFJ72_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480260.1"
                     /translation="MVRTIEAVIDEQGTVRLLEDVHLPSTRRALVTILEDAPAEQTSE
                     SVLLSEEALAEDWNRPEEDAAWSHLQPER"
     gene            complement(831..1586)
                     /locus_tag="VFJ72_12170"
     CDS             complement(831..1586)
                     /locus_tag="VFJ72_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480261.1"
                     /translation="MWDYVRFYSVVQRQSREARELWEEKLRSNIMEIDKSHDFSIPRG
                     HAELFFDYLKYREHDFENAQSLLRTEEEALAYCEEIGAKVKKVRTKSQDHHQSSAAMV
                     AAVAYIAGEHCLERSVGFDKAPNRRCVWLVNNQLHVTARNLDGAIPSLDSPEVVWEIK
                     EYWGKTSGGSKMSDAVYECALVGRELRDFEERSDAQRVQHVVFLDGKDQWASRKSDLK
                     RFIDLFHQGLIDHLFIGRDVETEWKALLDSLLP"
     gene            complement(1640..1927)
                     /locus_tag="VFJ72_12175"
     CDS             complement(1640..1927)
                     /locus_tag="VFJ72_12175"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7480262.1"
                     /translation="MILIFCRLSSSIPYSAAMTSKDRECRAFGTTVRLYRKQAGYSQE
                     ELADRAGIHRTYIGGIERGERNPTLTMIHRLARALGVPSHLLLEEPPKSNV"
     gene            1936..2829
                     /locus_tag="VFJ72_12180"
     CDS             1936..2829
                     /locus_tag="VFJ72_12180"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HET7480263.1"
                     /translation="MIKANVAPVLKWAGGKQGIASRLVELFPSGFDRYFEPFVGGGSV
                     LFTLRPQKAVIGDANDWLIDTYEAIREDYTQVARILDGLENTKERYLRVRSILPRTLP
                     LFRRAAYLIYLNKTCFRGLFRVNRQGQFNVPYGQYDRRYYDPANLKAVAEALKNVEFR
                     RGDFELCLRDVTEEDFVYMDPPYYKLGGYSDFNRYTRDQFRENDHFRLAALCRELDLR
                     GVRWAVSNSDTALIRTLFEGYHLNLIENRREINLNSQSRNIMELLITNYAPQSRPTQG
                     TLFDKFAPKTLDLVSRAPEHK"
     gene            complement(2849..4174)
                     /gene="senA"
                     /locus_tag="VFJ72_12185"
     CDS             complement(2849..4174)
                     /gene="senA"
                     /locus_tag="VFJ72_12185"
                     /inference="COORDINATES: protein motif:HMM:NF041186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1903257 - selenoneine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine synthase SenA"
                     /protein_id="HET7480264.1"
                     /translation="MAKTRSNTEELTAMILDSRKRTLELISDLDEEQMMGPQLRIVNP
                     PLWEIGHLAWFQERWNLRRITDDTFAPSDILANTDDLYDSMQVHHDTRWSLPLLTRED
                     TLAYMQKILDLTLERIRTEGPTPETDYFARLATYHEDMHGEAFTYTRNTHGLPAPPIS
                     DHDPSILEDQPTGPLEGDAKVPGGEFMLGASQDAPFAFDNEKWEHPVEVAPFRMSRAP
                     VTNEQFAAFVEDGGYRSNDLWSEAGRKWRDDTGAEHPVYWSHRDGEWRRRHFDRVEPL
                     PPHHPAMHVNWYEAEAYCRWAGRRLPTEAEWEMAASAEPAPDGKGITGHKRVYPWGDG
                     PPEPRHANLDGRLLGPVDVAAFPDGDSAFRLRQMIGNTWEWCSDDFGPYPGFVRDPYK
                     DYSEPWFGDHKVMRGGCWATRGRMLRNTWRNFATPDRRDIFAGFRTCAL"
     gene            4299..5252
                     /gene="senB"
                     /locus_tag="VFJ72_12190"
     CDS             4299..5252
                     /gene="senB"
                     /locus_tag="VFJ72_12190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenoneine biosynthesis selenosugar synthase
                     SenB"
                     /protein_id="HET7480265.1"
                     /translation="MKISLITPAGPKSRYGNRRTAERWASFLRDLGHEVTVEETWNGE
                     PAEVMIALHARRSHDSIARYAATHPDHPLIVALTGTDLYRDIHQDADARKSLDVATRL
                     IVLQDAGLMELDPRHRKKARVVYQSAEQICRQPPEETSFDVCVIGHLRAEKDPFRTAL
                     ASQLLPPSSRVRVIHAGGARDGALAAEAQAHMRENPRYQWLGEVSREKVRVLLSRARL
                     LVQSSLMEGGANAVSEALAAGLPVIASDIPGNVGMLGEDYPGYYPPGDERALARLLER
                     TEQDPDFYELLEAKCRARRHLTRPGNEREALRAVIQEFRVR"
     gene            5311..>5789
                     /gene="selD"
                     /locus_tag="VFJ72_12195"
     CDS             5311..>5789
                     /gene="selD"
                     /locus_tag="VFJ72_12195"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5362..5364,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HET7480266.1"
                     /translation="MDERTRIRLTKYSTKAGUASKFAPGVLEQVLDDLTPGSPSSAAD
                     RFMSSMETRDDAGFVPFGGGLLLQSVDFFPPIVDDPYRFGQIAAANALSDIYAMGGEP
                     ITAMNLVGFPSKLDLGILREILEGGRSKIDESGARLLGGHSVQDDEPKYGLSVTGFV"
BASE COUNT         1095 a         1792 c         1746 g         1156 t
ORIGIN      
        1 gtttcgtggg cgcgggcggc atcgggcagc agatcctcat ctccatgaac ctcttcgact
       61 acccgaaagt agctacactg gtaggtgcga ccatcatagt cgtgctgctc gtggaccgtt
      121 tctccgcggc ggtgcggcga cagctcgtct actgagcctc atcccgaagt agcgggttct
      181 gctgtagaat tacggtggag gtaagcgcat ggtacgaacc atcgaagccg taatagatga
      241 gcaagggacc gtcagactcc ttgaggacgt gcatctgccg tccacgcgcc gagcgctcgt
      301 gaccatcctc gaagacgcgc cagcggagca gaccagcgag agtgtcttgc tgagcgaaga
      361 agctctagcg gaagactgga accgtcccga agaggatgcc gcatggtcgc accttcagcc
      421 ggaacggtag tcctggttcc atttcccttc tccgacctct ccaggtccaa gctgcgtccc
      481 gccgttgtcc tcgccgatgc gggccgggga gactgggtac tatgccaggt gaccagcaac
      541 ccttactcgg acttgcgagc ggtcgcgctc tctgacgaag acttcagtag cggttcccta
      601 cgacggaaaa gctatgcacg ttcgggcaag ctattcagac ctgatcgtta ccaaagtcgg
      661 ccacctgaaa acggagttac tcggacgcat aaccaatgct acggtaaatc tcctgagatc
      721 taaccgataa acctcttcga ctatccgaaa gtggccaccc tcgtcggcgc cacgataatc
      781 gtcgtgctgc tcgtggaccg cttctcggcg gcgcgacagc ggctcattta ctaggggaga
      841 agcgaatcca acaacgcttt ccactctgtc tccacgtcac ggccgatgaa gagatggtcg
      901 atcaagcctt ggtggaacag gtcgatgaag cgcttcaggt ccgatttacg cgatgcccac
      961 tgatccttac catcaagaaa tacgacgtgc tgaacccgtt gcgcatctga acgctcctcg
     1021 aagtcccgca gctctcgtcc gaccagagcg cactcgtata cggcgtcgct catcttgctg
     1081 ccgccggagg tcttacccca gtactccttg atctcccaca ccacctcggg agaatctaga
     1141 gagggaatag ctccgtcgag gtttcgcgcc gtgacgtgaa gttggttatt gaccagccac
     1201 acgcagcggc ggtttggagc cttgtcgaac cccactgatc gctccagaca atgctcaccc
     1261 gcgatgtacg ctaccgccgc caccatcgcc gcggaggact gatgatgatc ttggcttttg
     1321 gtcctgacct ttttgacctt tgctccgatc tcctcgcagt aggctagggc ttcctcttcg
     1381 gttcgcagca ggctctgtgc gttctcgaaa tcgtgctcgc ggtatttcag gtagtcgaag
     1441 aacaactcgg cgtgtccacg ggggatcgag aaatcgtgac tcttgtcgat ttccattatg
     1501 ttgcttcgaa gcttctcttc ccagagctcc cgagcttccc tgctctgtct ctggacgacg
     1561 gagtagaacc tgacgtaatc ccacaaggga tgccgccaac ccaaatcccg ccacttttcg
     1621 ttaatattca accgttcccc taaacattgg attttggagg ttcttcgagt aacagatgcg
     1681 atggaacgcc cagagctctc gcaagacggt ggatcatggt gagcgtggga ttcctttcgc
     1741 cacgctcgat cccacctatg tatgtgcggt ggatccccgc cctgtctgcc aattcctcct
     1801 gcgaatatcc agcttgcttg cggtacaacc gcaccgtggt cccaaaagcc cgacactccc
     1861 ggtccttcga agtcatcgcc gcactatacg gaatgctact tgagagtcta cagaatatga
     1921 gtatcatacg gtctcatgat taaggcaaac gtagcaccgg tcttaaagtg ggctggaggc
     1981 aaacagggta tagcctctcg attagtcgag ttgttccctt ctggtttcga ccgctacttc
     2041 gagccattcg tgggaggcgg gagcgtcctg ttcacgctgc ggcctcagaa ggccgttatc
     2101 ggcgacgcaa acgattggct catcgacacc tatgaagcta tccgggaaga ctatactcag
     2161 gtggcgagaa tactcgacgg tcttgagaac acgaaagagc ggtatctgag ggttcggagt
     2221 attctgcccc gtacgcttcc gttgtttcgg cgggcggcct atttgatcta cttgaacaag
     2281 acgtgctttc gggggctatt ccgtgtaaac cggcaaggtc agttcaacgt accttatggt
     2341 cagtacgacc gacggtatta tgatcccgcc aaccttaagg cggtggctga ggctcttaaa
     2401 aacgtcgaat ttcgccgggg tgacttcgag ttgtgtttgc gcgacgtaac cgaggaggac
     2461 ttcgtgtaca tggacccgcc ctactacaag ctcggtggat actcggattt caatcgctac
     2521 acaagagacc agtttagaga gaacgaccac ttcaggctag ccgcgttgtg ccgggagttg
     2581 gacttgagag gagtgcgctg ggccgtcagc aacagtgata cggcgctcat ccggactctc
     2641 ttcgagggat accatctgaa cctcatcgag aaccgccggg agataaacct gaactcccaa
     2701 agccgcaata tcatggagct gttgatcacg aactacgcac cacagagcag acccacgcaa
     2761 ggaactttgt tcgataagtt cgcgccaaaa acgctggacc tcgtatcgag agcccctgaa
     2821 cacaagtaac cagacgaacc tcgctcagct acaaagcgca cgtcctgaac ccggcgaaga
     2881 tgtcccttct gtccggggtg gcgaagttgc gccaggtgtt acggagcatc cttcctcttg
     2941 tggcccagca gccaccgcgc attaccttgt ggtcgccgaa ccagggctcg gagtagtcct
     3001 tgtagggatc gcgcacgaag ccggggtacg gcccgaagtc gtccgagcac cactcccagg
     3061 tgttcccgat catctgccgt aacctgaagg cgctgtcgcc gtccgggaat gcggccacgt
     3121 ctaccggccc gaggagccgt ccgtcgaggt ttgcatgccg gggctccggc ggcccgtcgc
     3181 cccaggggta aacgcgcttg tggcccgtga tccctttgcc gtccggcgcg ggttcggcgg
     3241 aggcagccat ctcccactcc gcctccgtcg gcaggcggcg tccggcccag cggcagtacg
     3301 cctcggcctc gtaccagttg acatgcatgg ccggatggtg cggtggcagc ggctcgaccc
     3361 tgtcgaagtg ccgcctgcgc cactcgccgt cccggtgcga ccagtagacg gggtgctccg
     3421 cgccggtgtc gtcgcgccac ttgcggcccg cctcgctcca gaggtcgttg ctgcggtaac
     3481 ctccgtcttc cacgaaggcg gcgaactgct cgttggtgac gggggcgcgg gacatcctga
     3541 acggcgcgac ctcgacggga tgctcccact tctcgttgtc gaaggcgaag ggcgcgtcct
     3601 gagaggctcc gagcatgaac tcgccgccgg ggaccttcgc gtcaccctcc agaggtccgg
     3661 ttggctggtc ttcgaggatg gagggatcat ggtcggagat gggaggcgcg gggagcccgt
     3721 gggtgttgcg ggtgtaggtg aacgcctcgc cgtgcatgtc ctcgtggtag gtggcgaggc
     3781 gggcgaagta gtctgtctcc ggggtcgggc cttcggtgcg gattctttcc agggtgaggt
     3841 cgaggatctt ctgcatgtag gcgagggtgt cctcgcgagt aagtagcggg aggctccatc
     3901 gggtgtcgtg gtggacctgc atggagtcgt agaggtcgtc ggtgttggcc aggatgtccg
     3961 agggcgcgaa cgtgtcgtct gttatgcggc ggaggttcca gcgctcctgg aaccaggcca
     4021 gatgccctat ctcccagagc ggcgggttca cgatgcggag ttgcggcccc atcatctgct
     4081 cttcgtcgag gtccgagatc agctccagcg tccgcttgcg gctgtcgagt atcatcgcgg
     4141 taagctcttc cgtattgctt cgggtcttag ccatgtcctc cacactttcc atccggttca
     4201 cccttcgcac ccgcatgtta gcgcaaacct ggacattcat ggaccgggac gcgcgggttt
     4261 atatatagcc gcgcatttat gagacgatac acagaagaat gaagatctcc ctgattaccc
     4321 ccgccggccc gaagtcgcgc tacggcaacc ggaggaccgc cgagaggtgg gcttctttcc
     4381 tgcgggacct cggtcacgaa gtaacggtcg aggagacctg gaacggcgag cctgcggagg
     4441 tcatgatcgc gctccacgcc cgccgcagcc acgactctat cgcccgctat gccgccacac
     4501 acccggacca tcccctcata gtcgccctca ccggcaccga cctgtaccgc gacatccacc
     4561 aggacgcgga cgcgcgaaag tccctggacg tggctacgcg cctcattgtg ttgcaggatg
     4621 cgggccttat ggagcttgat ccgcgccacc gcaagaaagc gcgggtcgta tatcagtccg
     4681 ccgagcagat ctgccgccag ccgcccgaag agacgagctt cgacgtgtgc gtgatcggac
     4741 acctgcgggc cgagaaggac ccgttccgga cggcgctcgc ctcccaattg ctacccccct
     4801 cctcccgcgt ccgcgtcatc cacgccggtg gcgcccgcga cggggcgctc gcggcggagg
     4861 cgcaggcgca catgagggag aatccccgct accagtggct gggcgaagtc tcccgcgaaa
     4921 aggtgcgggt tttgctctcc cgagcccgtc tgctggtgca gagctctctt atggaaggcg
     4981 gggcgaacgc ggtctccgag gcgctggccg ccgggttacc ggtcatcgcc tccgacatcc
     5041 ccggcaacgt cgggatgctc ggcgaggact accccggcta ctacccgccg ggcgacgagc
     5101 gcgccctcgc ccggttgctg gagcggacgg agcaggatcc cgacttctac gagctgctgg
     5161 aagcgaaatg ccgggcccgg cgccatctca cgcggcccgg caacgagcgc gaggcgctga
     5221 gagccgtgat ccaggagttc cgcgtccgct agaggccccc gaggaatcgg ggctccgtcc
     5281 acaaacgctt ttgcggatat aatgccgggc atggacgaac gcacgcgcat aagactgacg
     5341 aaatactcga cgaaggctgg ttgagcctcc aagttcgctc caggggtcct ggagcaggtg
     5401 ctcgacgacc tgacgcccgg ctcgccgtct tccgcggcgg acaggttcat gagcagcatg
     5461 gagacccgcg acgacgcggg gttcgtaccg ttcggcggcg gcctgctgtt acagtcggtg
     5521 gacttcttcc cgccgatagt ggacgacccg taccgcttcg gccagatcgc cgcggccaac
     5581 gccctctccg acatctacgc gatgggtgga gagccgatca cggcgatgaa cctcgtcggt
     5641 ttcccgagca agctcgacct cggtatcctg cgcgagatcc tggagggcgg acgctcgaag
     5701 atcgacgagt ccggcgcgcg cctgctcggt gggcactccg tccaggacga cgaaccgaag
     5761 tacggcctct ccgtcaccgg cttcgtcga
//
LOCUS       DASSMB010000200         3680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1446451, whole genome shotgun sequence.
ACCESSION   DASSMB010000200 DASSMB010000000
VERSION     DASSMB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3680
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446451"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFJ72_12200"
     CDS             <1..264
                     /locus_tag="VFJ72_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480267.1"
                     /translation="SFTKVSEGPVLGAAMLASVGAGIYPDVQTAAQNMVHTERTIEPN
                     LEAHEAYKFYLERYIETYPQMKDEMHKMSRHEAAQSENAAEQA"
     gene            416..1771
                     /locus_tag="VFJ72_12205"
     CDS             416..1771
                     /locus_tag="VFJ72_12205"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HET7480268.1"
                     /translation="MNDERIEAALREATDTREVTIATGALASVDGVFGRSFGNRAAVV
                     VADEHTFAVAGEETRRNLEATGKTLEPYIFSGRPMLYAGYDNIETLIEALREHDAIPV
                     AVGSGTLNDIVKRAAHECDRPYMCVGTAASMDGYTSFGASISKDGRKQTLECPAPRAV
                     LGDVDVLVEAPAWMTASGFADLLCKVTAGADWIIADALGTEEIHPQAWSLVQDNLREW
                     TARPAELRAGDGDAMDGLMEGLIMAGLAMQVAASSRPASGAEHMFSHLWEMEGLGHDE
                     DPPLSHGFKVGVGSIAVAALYERVLQRDLTNLDVDGIANDWPAWETVEREVRATHTVP
                     GLDEYAVEESRAKYVDADQLRRRLEQLREFWPGLRERLEGQLMPADELRSLLRAAGCP
                     VSPAEIGLSWDDFRATYARARTIRRRYNVLDLAVETGVFDECVEELFAPGGFWARETG
                     S"
     gene            1812..3458
                     /locus_tag="VFJ72_12210"
     CDS             1812..3458
                     /locus_tag="VFJ72_12210"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7480269.1"
                     /translation="MDQGDGEEAQAFTVDGRREGIGGKMAEGPYLMGIDFGTGGARVG
                     IFDQEGTPLVFHGVEWETKHPRPGWAEQDPDDWWSCLVQATKGALEESGVAPEEIAGI
                     AVDTTSCTVVAMDENDKHLRPAIMWMDVRASDQAERASNTGDPALKYNGYGAVSAEWG
                     LPKALWLKENERETYDNARHICDCQDWLNNRLTGEWVASINVVAAKYHYDRGVGGWPV
                     SFYEAVGVEDVLEKFPDPVLDLGAVVGELRKEAAEELGLKAGTPVAQGAIDGYSGAVG
                     LGVTEPGKMALITGSSHSMLGQSAEPIHGRGFWGAYTDAVIPGQYTVEGGQVSTGSVV
                     AWFKNQFAMKANQEAGERGEDPYVILNEMAENIPIGSNGLVVIDYFQGNRTPHTDSLA
                     RGIMWGLSLSHTEAHMFRAILEGICYGTEHILRTMRGHDFEPKLNVVSGGPAKSELWM
                     QMHADVSGVPISMTRVSEGPVLGSAMLASVGAGIYPDVQAAAQNMVHTERTIEPNLEA
                     HEAYKFYVDRYIETYPAMKELMHKTVRHEASGHETAAKKA"
BASE COUNT          714 a         1073 c         1335 g          558 t
ORIGIN      
        1 tccttcacga aggtgagcga aggtccggtc ctgggcgcgg cgatgttggc ctcggtcggc
       61 gcggggatct acccggatgt acagaccgcc gcccagaaca tggtccacac cgagcggacc
      121 atcgagccga acctcgaagc gcacgaggcg tacaagttct acctggagcg ctacatagag
      181 acctatccgc agatgaaaga cgagatgcac aagatgtcgc ggcacgaggc ggcccagagc
      241 gagaacgccg cagagcaggc gtaggcaccg tgtccgcagt tcctgaccgc cgggggcgaa
      301 ggcgacaggg gctgttggta aggggaaacc tggctggagc tacgaggtcg agcggcggag
      361 aagatgtgac gacgagcgag aaaccgttcc tttcgcacga aatgggagat agccggtgaa
      421 cgatgaacgc atcgaggcag cactacgcga ggcgaccgac acgcgagaag tcacgatagc
      481 cacgggggcg ctcgcttcgg tagacggcgt attcggacga agcttcggga atcgggcagc
      541 ggtcgtggtc gcggacgagc acacgttcgc ggtcgcgggc gaggagacgc ggaggaacct
      601 ggaggcgacc ggaaagaccc tggagccgta catcttctcc ggccggccga tgctgtacgc
      661 cggctacgac aacatcgaga cacttataga agcgctgcgc gagcacgacg ccatcccggt
      721 cgccgtcggc tcggggacgc tcaacgacat cgtcaagcgg gcggcccacg agtgcgaccg
      781 gccctacatg tgcgtcggta ccgcggcctc gatggacggc tacacgtcgt ttggcgcgtc
      841 gatcagcaaa gatgggcgca agcagacgct ggagtgcccg gcgccgaggg cggtgctggg
      901 cgacgtggat gtcctggtgg aggctcccgc ctggatgacg gcctccggct tcgccgacct
      961 cctgtgcaag gtgacggcgg gcgccgactg gataatcgcc gatgcgctgg gaaccgagga
     1021 gatccacccg caggcctggt cgctggtaca ggacaacctg cgcgagtgga ccgcgcgtcc
     1081 ggctgagttg cgcgccgggg atggagatgc gatggacggg ctcatggagg gcctgatcat
     1141 ggcgggcctg gcgatgcagg tcgccgcctc ttcccggccg gcgtccggcg ccgagcacat
     1201 gttcagccac ctgtgggaga tggaggggct gggccacgac gaagatccgc cgctctcgca
     1261 cggattcaag gtgggggtgg gctccatcgc cgtcgccgcc ctgtacgagc gggtactaca
     1321 gcgcgacctg accaacctgg acgtggacgg gatcgccaac gattggccag cctgggagac
     1381 ggtcgagcga gaggtgcggg ctactcacac cgtgccgggc ctggacgaat acgccgtcga
     1441 ggagagccgc gccaaatacg tggatgcaga tcaactcagg agacggctgg agcaactccg
     1501 ggagttctgg ccggggttgc gcgagcggtt ggaggggcag ttgatgccgg ccgatgagct
     1561 acgaagtctg ctgcgggcgg cggggtgtcc cgtcagcccg gccgagatcg ggttgagttg
     1621 ggacgacttc agggcgacgt atgccagggc gcggacgatc cggcgccggt acaacgtcct
     1681 ggatctcgcc gtcgagaccg gtgtcttcga tgagtgtgtc gaggaactgt tcgcgccggg
     1741 cggtttctgg gcccgtgaga ctggctcatg agaggttggc cagggaaaca gaggaaaatc
     1801 gcatccggag gttggatcag ggagatggcg aagaggcaca ggcgttcacg gtggacggac
     1861 gccgggaggg gataggagga aagatggccg aaggtccgta cttgatgggg atagatttcg
     1921 ggacgggtgg cgcaagggtc ggcatcttcg accaggaggg tacgcccctg gtcttccacg
     1981 gcgtcgagtg ggagacgaag cacccgcgtc cgggctgggc cgagcaagac ccggacgact
     2041 ggtggtcgtg cctggttcag gcgacgaaag gcgctctgga ggagagtggc gtcgcgcccg
     2101 aggagatcgc gggcatcgcc gtggacacta ctagctgcac cgtcgtggcg atggacgaga
     2161 acgacaagca cctgaggccc gccataatgt ggatggacgt gcgcgcctcc gaccaggccg
     2221 agcgcgcctc caacaccggc gacccggcgc tcaagtacaa tggatacggt gccgtttcgg
     2281 ccgagtgggg tctcccgaaa gccctgtggc tcaaggagaa cgagcgcgag acctacgaca
     2341 acgcccggca catctgcgac tgtcaggact ggctcaacaa ccgcctcacg ggtgagtggg
     2401 tggcctccat caacgttgtc gccgccaagt atcactacga ccgcggagtg ggcggctggc
     2461 ccgtgagctt ctacgaggcc gtcggggtcg aggacgtgct ggagaagttc ccggatcccg
     2521 tgctcgacct gggagcggtc gtcggggagt tgcgcaagga agcggcggag gagctcggcc
     2581 tgaaggccgg cacgccggta gcgcaggggg ccatagacgg ttactcgggg gccgtagggc
     2641 tcggggttac cgagcccggc aagatggcgc tcatcacggg gtcttcgcac tccatgctcg
     2701 gacagagcgc cgagcccatc cacggcaggg gcttctgggg cgcctacacg gacgccgtga
     2761 tccccggcca gtacacggtc gagggcggtc aggtctccac gggttccgtc gtggcctggt
     2821 tcaagaacca gttcgccatg aaggccaacc aagaggccgg ggagcgcggc gaggacccct
     2881 acgtcatcct caacgagatg gcggagaaca tacccatcgg ctccaatggc ctggtggtca
     2941 tcgactattt ccagggcaac cgcactcccc acaccgactc gttggcgcgc ggcattatgt
     3001 gggggctttc gctcagtcac accgaggcgc acatgttccg tgcgatcctg gagggcatct
     3061 gctacggcac cgagcacatc ctccgaacca tgcgcggcca cgatttcgag ccgaagttga
     3121 acgtcgtctc aggcggtccc gccaagagtg agctgtggat gcagatgcac gccgacgtct
     3181 cgggcgttcc catctccatg acgcgggtga gtgagggtcc ggtcctgggc tcggctatgc
     3241 tggcctcggt cggcgcgggg atctacccgg acgtccaggc cgccgcccag aacatggttc
     3301 acaccgagcg gaccatcgag ccgaacctcg aagcgcacga ggcgtataag ttctacgtgg
     3361 accgctatat cgagacctat ccggcgatga aggagctgat gcacaagacg gtccgtcacg
     3421 aggccagcgg ccacgagacc gcggcaaaga aagcatagga tgcgtgatcg gaggcgtcca
     3481 cacgaaggca acgacgaggg agcgagtgag gctgcatgaa gattgtatcc tgtgcgggcc
     3541 gcaaggttcg tttcgatata aaggaggcgg gaagttgacg accgtaacag tcctggggtc
     3601 gggcataatg gcgacggcgc tggcgtggcc gctctcggac aacggccacg aggtacggct
     3661 ggtggggact cacctcgacc
//
LOCUS       DASSMB010000201         5057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1826583, whole genome shotgun sequence.
ACCESSION   DASSMB010000201 DASSMB010000000
VERSION     DASSMB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826583"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VFJ72_12215"
     CDS             <1..229
                     /locus_tag="VFJ72_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480270.1"
                     /translation="SASNQIGPHLSYRRYLYLFPDLGDARYVILDETEPGYDTYINPV
                     LNLQSTRELRENPAYRKIFDKNGVLVFEKRR"
     gene            complement(240..836)
                     /locus_tag="VFJ72_12220"
     CDS             complement(240..836)
                     /locus_tag="VFJ72_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480271.1"
                     /translation="MAYMINRYAPLVVISFSIFLLAGCSALTGDPREQANESISAAND
                     SIAKHNRLFEQARDTYADVKKKVEAGDDPSKEKKRITDAKDTLQEARTNLKDAKDSLD
                     EVQDLDVDPAVKKYSSTLSDAMDAQLAAEAKEIEFYGILEKDPALKDQRDKALKLLSE
                     VGDGYSKAEKAYGEARQIANKNPKILGPAKTSKSGSGG"
     gene            835..1266
                     /locus_tag="VFJ72_12225"
     CDS             835..1266
                     /locus_tag="VFJ72_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480272.1"
                     /translation="MSEGSLPPFKSIWLRFSPVALVRGPLGRVSKRLRRRRAEPVDPD
                     LRSRAAAAVKEHSRIHAALFERASRLREKAERLESAGTPSESAHNRAARAEWEATESL
                     ADLRASFAVSDGEEGARTFDLVVRESYPALGSSYVRPERRS"
     gene            complement(1284..1736)
                     /locus_tag="VFJ72_12230"
     CDS             complement(1284..1736)
                     /locus_tag="VFJ72_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480273.1"
                     /translation="MFGRKSRAEKLREQAESSSLIPTAAIAGFLSGAKPIAERLLYDD
                     DLRDNIRTFIDAAREILDEINDESPTDIISKLWDDDKLRSRVETATTAAQQGSKRVRG
                     EKVKADRGSGRLFFLIVAAALGFLFLNPKTGPEVRRLTKQALGAVTGE"
     gene            complement(1802..5002)
                     /gene="ileS"
                     /locus_tag="VFJ72_12235"
     CDS             complement(1802..5002)
                     /gene="ileS"
                     /locus_tag="VFJ72_12235"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET7480274.1"
                     /translation="MAFDKVRPQVNFPKLEEDVLDLWRRESTFEKSVQSRPEDQPFVF
                     YEGPPTANGKPGFHHALARAFKDLIPRYKTMQGYRVVRKGGWDTHGLPVELQVEKELG
                     LTGKDDIERYGVEEFNKLCRKSVFRYVEDWQKMSERLGFWVDMDDPYQTLDNSYIQSV
                     WWALKTLHEKDLLYEGYKVSAHCPRDQTSLSSAEVALGYQQYPDPVVDPSVYVKLPLV
                     DEDHTSLLIWTTTPWTLISNAAVAVHPNVEYATVEVDNERLVIATDLLDKVLGEGEHH
                     IVACTKGHYLEGKKYRRPFDYMPVEESKSLWTVVLGEYVTTTEGTGLVHTAPAYGEDD
                     ARVGRRYGLPTIHPVKPDGTFDERVGPFAGQFVKGADKGLVAELRESGLLFRAEEYEH
                     AYPHCWRCGTPLLYYAKKAWYARTTAVLDDLLSENEGINWVPDHIKWGRFGDWLKNNI
                     DWALSRERYWGTPLPIWRTESGATVVIGSAEELRELAIDPVPEDLHRPYIDEVRIKHP
                     ETGEEARRVPEVLDVWFDSGSMPFAQWAYPHSGEAGAQRFERQFPADYICEAVDQTRG
                     WFYSLLAVSTMLFGKSSFKTCLVLGHILDSEGKKMSKSLGNVIDPWAIFGKQGADALR
                     WALYTATSPGNTRRFSEDQVDEAVRKYLLTLWNTYSFFVTYARIDGFDPLRDYVEPRD
                     RSLMDRWALSELQLTVKTLTERLDAYDVTAAGRAIGEFVDELSNWYVRRSRRRFWKGE
                     DDQDKKAAHSTLYECLTAVVRLTAPFTPFVAEEIYQNLTVNADADAPGSVHLASWPLV
                     DEALVDRSLSGRMEAARRVVALARAARNSAAIKTRQPLREVVVVGEASNNESDGFREG
                     VESLKEIILDELNVKELRFGEPEDVVAYDLKPNLGIVGPKYGKLVPGIRAALAEAPVE
                     VGARAAAGESVPVSVNGEEVTLSPEELLVEPTERTGYALEREGDLSVALRTELDPELV
                     DEGLVRELVHKVQNLRRERGFEIEEGISVGLSGSERFERLLQGSWGEYFKAEVLARRL
                     DLDGTPDGTPDGAAHVSVDGEELRVSLEPIGGASVR"
BASE COUNT          801 a         1678 c         1558 g         1020 t
ORIGIN      
        1 ctccgcgagc aatcagatag gaccgcacct ctcctaccgg cgctacctct acctcttccc
       61 ggacctgggg gacgcccggt acgtgatcct ggacgagacc gaaccgggct acgataccta
      121 tatcaacccg gtcctcaacc ttcagtcgac cagggagttg cgcgagaacc cagcctaccg
      181 aaaaatcttc gacaagaacg gtgtgctggt cttcgaaaag cgccggtagc cgctaccgtt
      241 tagccgccgg aaccgctctt agaggtcttt gcggggccga ggatcttggg gttcttgttg
      301 gcgatctgac gggcctcgcc gtaggctttc tccgccttgc tgtagccgtc tccaacctct
      361 gagagtagct tgagggcctt gtcgcgctga tctttgaggg ccgggtcttt ttccaggatt
      421 ccgtagaact ctatctcttt ggcttcggcg gctagctggg cgtccatcgc gtcggagagc
      481 gtgctggaat acttcttgac cgccgggtcc acgtcgaggt cttgaacctc atcgagcgag
      541 tccttcgcgt ccttcaggtt ggtccgggct tcctgcaggg tgtccttcgc atcggttatg
      601 cgctttttct ccttcgaggg gtcgtcgccg gcctcgacct tcttcttcac gtcggcgtag
      661 gtgtcgcggg cctgttcgaa gagccggttg tgcttggcta tggagtcgtt tgccgccgag
      721 atggactcgt tagcctgctc tcgggggtcg cccgtaaggg ccgagcaccc ggcgagaagg
      781 aatatcgaga aggatattac gacgagcggc gcataacggt ttatcatgta tgccatgagc
      841 gaaggaagtc taccgccttt caagtccatc tggttacgct tctctcccgt cgcgttggtc
      901 cgtggtccgc tcggcagggt ctccaagcgc ctgcggcgcc ggagggcgga gccggtggac
      961 ccggacctgc gttcccgcgc cgctgccgct gttaaggagc actccaggat ccatgcggcg
     1021 ctcttcgagc gcgcctccag gctgcgggag aaggccgaac gcctcgaaag cgccgggacg
     1081 ccgagcgaga gcgctcacaa ccgtgccgcc cgcgcggaat gggaagccac ggagagcctg
     1141 gcggacctgc gcgcttcctt cgcggtctct gacggagagg agggcgcgcg gaccttcgac
     1201 ctggtggtaa gggagagcta tccggcgctc ggctcgtcgt atgttcggcc ggagcgccgt
     1261 tcctagctct ctaaccaatc cttttattcg ccggtaacgg cgccaagagc ttgcttggtc
     1321 agcctgcgga cctcgggtcc ggtcttcggg ttcaggaaga ggaatcccag agcggcggcg
     1381 acgatcagga agaacagcct gccggagccg cgatccgcct tcaccttctc tccccggacg
     1441 cgcttggagc cctgctgggc ggcggtggta gcggtctcca cccgtgaacg tagcttgtcg
     1501 tcgtcccaga gcttggagat tatgtccgtc gggctctcgt cattgatctc atcgagtatc
     1561 tcgcgggccg cgtcgatgaa ggtgcggatg ttgtcccgga ggtcgtcgtc gtagagcagc
     1621 cgctcggcga tgggcttggc ccccgacaag aaaccggcta tcgccgcggt cgggatgagc
     1681 gagctcgact cggcctgctc tcttagcttc tctgcccggc tctttctgcc aaacatcgtt
     1741 accttcctct ctccgctttc ctggcctgca atcgctatat catacccgtt ttgaagcgct
     1801 tctatctgac ggatgcgccg ccaatcggct caagactgac gcgcagttct tcgccatcca
     1861 cgctcacgtg cgcggcgcca tccggcgtac cgtccggcgt accgtcgagg tccaacctcc
     1921 gcgcgagcac ctcggccttg aagtactcgc cccagctacc ctggagcagc cgctcgaacc
     1981 gctcgctccc ggagagcccg acggagatcc cctcttctat ctcgaaaccc ctctcccgcc
     2041 gcaggttctg gaccttatgg accagctccc gcacgagccc ctcgtctacc aactcagggt
     2101 ccagctccgt gcgcagagct accgagaggt ctccctcgcg ctccagcgcg tagcctgtcc
     2161 gctccgtagg ttcgacgagc aactcctccg gcgacagtgt gacctcctcg ccgttcacgc
     2221 tcaccgggac gctctcaccc gccgcggccc gcgcgccgac ctccaccgga gcctccgcga
     2281 gcgccgcccg gatgccgggg accagcttcc cgtacttcgg gccgacgatg ccgaggttcg
     2341 gttttaggtc ataggcgacc acgtcctccg gctccccgaa acgcagctcc ttgacgttca
     2401 gctcgtccag gatgatctcc ttgaggctct ccacgccctc gcggaaccca tcgctttcgt
     2461 tgttagacgc ctccccgact accactacct cgcgcagcgg ctgacgggtc tttatggcgg
     2521 ctgagttgcg ggccgcccgg gcgagcgcca caacccgccg cgccgcctcc atcctgccgg
     2581 agagacttct gtccaccagc gcctcgtcca ccagcggcca gctcgccagg tggacgctgc
     2641 ccggagcgtc ggcgtccgcg ttgaccgtga ggttctggta gatctcctcc gccacgaacg
     2701 gcgtgaaggg cgccgtgagc cgcaccacgg cggtgagaca ctcgtagagc gtcgagtggg
     2761 cggccttctt gtcctggtcg tcctcgccct tccagaaccg ccgccggctg cgcctgacgt
     2821 accagttgga cagctcgtcc acgaactcgc cgatggcgcg cccggcagcc gtaacgtcgt
     2881 aggcatccag ccgctccgtt agggtcttga ccgtcagctg caactccgat agcgcccagc
     2941 ggtccatgag gctgcggtcc cgcggctcca cgtagtcccg cagagggtcg aagccgtcta
     3001 tgcgggcgta ggtgacgaag aaggagtagg tgttccagag ggtcaggagg tactttcgga
     3061 cggcctcatc tacttgatcc tcgctgaaac gccgcgtgtt gccggggctc gtcgcggtgt
     3121 agagcgccca gcgcagcgcg tcggcgccct gctttccgaa gatggcccac gggtctatga
     3181 cgttacccag ggacttgctc atcttctttc cctcggagtc caggatgtgt ccgagaacaa
     3241 ggcaagtctt gaaggaagat ttgccgaaga gcatcgtcga gacggcgagc agcgagtaga
     3301 accagccgcg cgtctggtcc acggcctcgc agatgtaatc cgccgggaac tgacgctcga
     3361 accgctgcgc gcccgcctcg ccgctgtgcg gataggccca ctgagcgaac ggcatgctcc
     3421 ccgagtcgaa ccacacgtcc agcacctccg ggacgcggcg ggcctcctcg ccggtctcgg
     3481 ggtgcttgat gcggacctcg tctatatagg gacgatgcag gtcctccggg acagggtcta
     3541 tcgccagctc ccggagttcc tccgcgctcc cgatcacgac cgtcgcgccg gactctgtcc
     3601 gccagatggg gagcggcgtg ccccagtagc gctcgcggct tagggcccag tctatgttgt
     3661 tcttgagcca gtcgccgaag cggccccact ttatgtggtc ggggacccag ttgatcccct
     3721 cgttctccga gagcaggtca tccagcacgg ccgtggtccg ggcataccag gctttcttgg
     3781 cgtaatagag gagcggcgtc ccgcagcgcc agcagtgcgg gtatgcgtgc tcgtactcct
     3841 ccgcccggaa aagcagaccg ctctcgcgaa gctccgcgac cagtcccttg tcggcgccct
     3901 tcacgaattg cccggcgaag gggccgaccc gctcgtcgaa ggtcccgtcg ggttttaccg
     3961 gatggatcgt gggcagcccg tagcgccgtc cgacgcgcgc gtcgtcctca ccgtaggccg
     4021 gcgcggtgtg gacgaggccg gtaccctcgg tggtcgtcac gtattctccg aggacgacgg
     4081 tccacaggct cttgctctct tcgactggca tgtagtcgaa cgggcgccgg tacttcttgc
     4141 cttccaggta gtggcccttt gtacaggcta ctatatgatg ctcgccttct cctagcacct
     4201 tatcgagcag gtccgtagca atcactaacc gctcgttatc tacctcgacc gtcgcatact
     4261 cgacgttcgg gtggacggcg acggcggcgt tggagatcaa ggtccagggc gtagtggtcc
     4321 agatgaggag gctggtatgg tcttcgtcta cgagcgggag tttgacgtag acgctcgggt
     4381 ccacgacggg gtccgggtac tgctggtatc cgagggccac ttcagcggag gagagggagg
     4441 tctgatctcg cgggcagtgc gcactgacct tgtagccttc gtagagcagg tctttctcgt
     4501 gcagcgtctt gagcgcccac cagacgctct gaatgtagga attatcgagg gtctggtagg
     4561 ggtcgtccat gtccacccag aagccgagcc gctcactcat cttctgccag tcctcgacgt
     4621 agcggaagac ggacttgcgg cacagcttgt tgaactcctc gacgccgtag cgctcgatgt
     4681 cgtccttgcc ggtgaggccg agttctttct cgacctggag ctccacgggc aggccgtgcg
     4741 tgtcccagcc gcccttgcgc acgacccggt atccctgcat ggtcttgtag cgggggatca
     4801 ggtccttgaa cgctctcgcc agcgcgtggt ggaaccccgg cttaccattg gccgtcggcg
     4861 gtccctcgta gaatacgaag ggctggtcct cgggacggct ctgtacagac ttctcgaacg
     4921 tactctcccg ccgccagagg tccagcacgt cctcctccag cttcgggaag ttgacctgcg
     4981 gacgaacctt gtcgaatgcc atgcacgctc ctcaatatga aagctcccgt ccccggctaa
     5041 cgccagacgg agcgacg
//
LOCUS       DASSMB010000202         3276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1060602, whole genome shotgun sequence.
ACCESSION   DASSMB010000202 DASSMB010000000
VERSION     DASSMB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060602"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..837)
                     /locus_tag="VFJ72_12240"
     CDS             complement(31..837)
                     /locus_tag="VFJ72_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7480275.1"
                     /translation="MPGTETRVFDLEQPRTDRMPIHSAHQQAGYSYLLHRHHESEYRP
                     AESGPRSGAAGIIICGEHTGTHIDALCHQAGDLKLYGDIAVSEVQTSRGFSKLAVEEI
                     PPILAPGVLLDVAAKDGVDSLETGHAVTAEDLDECCEQQGVSVEAGSVLIVRTGNGRY
                     WEDTERYLAGPGMDASASYWAAEKGVLAVGADNMAWDAPGVKDPELGCLLPGHLILLV
                     RHGIYIVENLVLEELAAAGHHRFEFVCAPLKLVGATGSPVRPLAVVSGSM"
     gene            974..1768
                     /locus_tag="VFJ72_12245"
     CDS             974..1768
                     /locus_tag="VFJ72_12245"
                     /inference="COORDINATES: protein motif:HMM:NF003969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HET7480276.1"
                     /translation="MTTGLRVSDPESLWEACRTGGWDRPTPGVCGEYVQTNLVVLPHA
                     DAFDFLRFCGRNPKPCPLIEATDPGDPEPKLTAPGADLRTDLPRYRVYRDGRLTDEQT
                     DIRELWRDDFVSFLIGCSFSFEEALLAAGVPVRHIEAGTNVPMYRTNVPCTPAGRFAG
                     PLVVSMRPIPAHLVPLAVTTTARVPQVHGAPVHVGDPDALGISDLDRPDYGEPVKIRS
                     GELPVFWACGVTPQAVALEAAPEVMITHSPGHMFVTRMTNRELLAS"
     gene            1786..>3276
                     /locus_tag="VFJ72_12250"
     CDS             1786..>3276
                     /locus_tag="VFJ72_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET7480277.1"
                     /translation="MQTESKKVPGKTRLGVDVGGTFTDLVALGDGKLITAKVPSTPRD
                     QSEGVMNAIETSEVDASSVTALAHGMTVATNALLERRGAKMALVTTKGFRDVLEIARQ
                     NRPSLYDLSIDRPPPLVPRDLRFTIKERMGPDGEIEPLDEESLREAIKGVRDANVESV
                     AVCLLFAFAHPEHEKRVGEALREALPDVHVSLSSEVLPEFREYERFSTTAADAYLAPK
                     LAAYLKNLAGKAEEDGVPSPLIMQSSGGVVRVEDAVSDAAGCVLSGPAGGVVGAAYVG
                     SLGGYEDLLTFDMGGTSTDVAPIISGEAQTTTETVVAGVPIKLPMVDVHTVSAGGGSI
                     AWADAGGALRVGPHSAGAEPGPAAYGKGGEEPAVTDANLFLGYLNDGAELGGEVVLRR
                     DLSENALSTLGERLGLPAEEAALGIVRVANAEMIRALRVISVERGLDPREFALLAFGG
                     AGGLHACALAEELGMETVLVPRAGGVLSALGLAISDLRRDYVRPYLS"
BASE COUNT          536 a         1116 c         1098 g          526 t
ORIGIN      
        1 atctccatgt gtctctcctt tcccggttgt ttacatacta ccggagacga cggccagcgg
       61 cctcaccggg gagcccgtcg ctcccacgag cttgagcggc gcgcagacga actcgaaacg
      121 gtggtggccc gcggcggcca actcttccag caccaggttc tccacgatgt agataccgtg
      181 tctcacgaga aggatcagat gtccgggcag aaggcagccc aactccgggt ccttcacacc
      241 cggcgcgtcc caggccatgt tgtcggcccc gacagccagc acgcccttct ccgccgccca
      301 gtacgacgcg ctcgcatcca tcccaggacc ggcgaggtag cgctcggtgt cctcccagta
      361 gcggccgttt ccggtcctca cgatcagcac gctcccggcc tccacgctca cgccctgctg
      421 ttcgcagcac tcgtcgaggt cttcggcggt gacggcatgg ccggtctcta gcgaatccac
      481 gccgtctttc gcggccacgt cgagaaggac tccgggggcc agaatcggcg gtatctcctc
      541 gacggcgagc ttgctgaagc cgcgcgaggt ctggacttcg ctcacggcga tatcgccata
      601 gagcttgagg tctccggcct gatgacagag ggcgtctata tgtgtcccgg tatgttcgcc
      661 gcagataatg atcccggctg cgcccgagcg cgggccggac tcggcgggcc ggtactcgct
      721 ctcgtggtgg cggtgcagca ggtaggagta accggcctgc tggtgggcgg aatgtatcgg
      781 catcctgtcg gtgcgcggct gctccaggtc gaagacacga gtctctgttc ccggcataag
      841 gctcccctca ctctctgtcc gcatccaggt tagccgcctc tcccgccacg cgccagcggg
      901 cgcaggtcgc gccggaagcg tcgaatgctg ctagagtttt accggaggag agagacggac
      961 gagaggtgag gttatgacca ccggcctgag agtatccgat cccgagagtc tctgggaggc
     1021 gtgccggacg ggcgggtggg accgtcccac accgggcgtc tgcggggaat acgtccagac
     1081 aaacctggtg gtactgcccc acgccgacgc cttcgacttc ctgcggttct gcgggcgcaa
     1141 cccgaagccg tgcccactta tagaggccac cgaccccgga gacccggagc cgaagctgac
     1201 ggcccccggc gccgacctgc ggacagacct tccacgctac cgcgtctacc gcgacggcag
     1261 gctcaccgac gagcagacag acatccggga gttgtggcgc gacgattttg tgtcgttcct
     1321 gattggttgc agcttctcct tcgaggaggc gcttctcgcg gctggcgtgc cggtacgcca
     1381 catcgaagcg ggcacgaacg tgccgatgta ccggacgaac gtcccctgca cgccggccgg
     1441 gaggttcgcc gggccgctag tggtctcgat gcgtccgatc ccagcccatc tagttcccct
     1501 ggcggtgacg accaccgcgc gcgtcccgca ggtccacggc gcgccggtcc acgtcgggga
     1561 cccggacgct ctgggcattt cggacctgga ccggccggac tatggggagc cggtgaagat
     1621 acggtcgggc gagttgccgg tgttctgggc gtgcggggtc acgccgcagg cggtcgcgct
     1681 ggaggcggca ccggaggtca tgatcacaca ctcccccggc cacatgttcg ttacccgcat
     1741 gaccaacaga gagctgctgg cctcctagag aagagactac agccgatgca gactgagagc
     1801 aaaaaagtcc cgggcaagac acgcctcggc gtagacgtcg gcggcacctt caccgacctc
     1861 gtcgccctcg gcgacggcaa gctcattacc gccaaagtcc cctccactcc acgcgaccag
     1921 tccgagggcg ttatgaatgc gatcgagacc tccgaagtgg acgcatcctc cgtcaccgcc
     1981 ctcgcccacg gcatgaccgt cgcaaccaac gccctgctcg aacgccgggg cgcgaagatg
     2041 gcgctcgtca ccaccaaggg cttccgggac gtgctggaga tagcccgcca gaatcgcccc
     2101 tcgctctacg acctttcgat agaccgccct cccccgctcg tcccgcgcga cctgcgcttc
     2161 accatcaagg agcggatggg accggacggc gagatagagc cccttgacga ggagagcctg
     2221 agagaagcca ttaaaggcgt gagagacgcg aatgtcgagt cggtcgccgt atgcctcctc
     2281 ttcgccttcg cgcacccgga gcacgaaaaa cgagttggag aagccctgcg tgaggcgctg
     2341 ccggacgttc acgtctccct gagcagcgag gtgttgccgg agttccggga gtacgagcgg
     2401 ttctcgacga ccgccgcaga cgcctacctg gccccgaagc tcgccgccta tctgaagaac
     2461 cttgcgggga aggcagagga ggacggggtt ccgagcccct tgatcatgca gtcatccggc
     2521 ggcgtggtgc gggtggagga cgcggtctcg gacgcggcgg ggtgcgtgct ctcggggccg
     2581 gcgggcgggg tcgtcggcgc ggcctacgtc gggtcgctgg gcggatatga ggaccttctg
     2641 accttcgaca tgggcgggac gagcacggac gtcgccccga tcatctcggg cgaggcccag
     2701 accacgacgg agacggtcgt cgccggagtc cccataaagc tcccgatggt ggacgtacac
     2761 accgtcagcg cgggcggcgg ctccatcgcc tgggcggacg cgggcggcgc cctgcgcgtc
     2821 ggcccccact cggcgggcgc ggaacccggt cccgccgcct acggcaaggg cggcgaggag
     2881 cccgccgtca ccgacgcgaa cctcttcctc ggctacctaa acgacggcgc ggagctcggc
     2941 ggcgaggtgg tgctgcgccg cgacctctcg gagaatgcgc tctctacttt aggcgagaga
     3001 ctcgggctcc cggcggagga ggcggcgctg ggcatcgtcc gcgtggcgaa cgcggagatg
     3061 atccgagcat tgcgggtcat cagcgtggag cgcggcctgg accccaggga gttcgcgctg
     3121 ctggcgttcg gcggggcggg cgggctgcac gcctgcgcgc tcgccgagga gctgggtatg
     3181 gagaccgtgc tcgtaccgcg cgccggagga gtcctctccg cgctcgggct cgccatctcc
     3241 gacctgcgcc gcgactacgt gcgtccgtac ctctcc
//
LOCUS       DASSMB010000203         6993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_169702, whole genome shotgun sequence.
ACCESSION   DASSMB010000203 DASSMB010000000
VERSION     DASSMB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169702"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFJ72_12255"
     CDS             <1..252
                     /locus_tag="VFJ72_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET7480278.1"
                     /translation="LDQALRRAIEPHYPELKEIHLSNYKVRILDEHRATAATTRVLID
                     STDGKRVWGAVGVGENIIEASWQALVDGLEYGVNGTKKG"
     gene            complement(313..1089)
                     /locus_tag="VFJ72_12260"
     CDS             complement(313..1089)
                     /locus_tag="VFJ72_12260"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7480279.1"
                     /translation="MDVQNGATQDRVAIITGGSRGLGKNMVRSIAGRGTDVIFTYQSN
                     RAAADELVDELEGMGRRAAALQLDVGDASTFDAFVEEVREVLGDWSVDRFNFLVNNAG
                     IGVHASIAETTEEQFDQLFDIHVKGPFFLTQRLLPLMDDGGRIVNVSTGLARFALPGH
                     AAYASAKGAVEVMTRYMALELGPRRIAANTIAPGAIETDFSGGAVRDNPEMNEMVASD
                     TALGRAGLPDDIGGVVASLLSPEMGWVNAQRIEASGGQHL"
     gene            1214..1918
                     /locus_tag="VFJ72_12265"
     CDS             1214..1918
                     /locus_tag="VFJ72_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7480280.1"
                     /translation="MVSKRASTRDRIVRAAARLLAEGGREAVSTRAVAAAAGVQAPTI
                     YRQFGDMRGLLDEVASFGFSVYLRDKTTRERAEDPVEDLRRGWDLHVGFGLANPAFYT
                     LMYGDPRPRAEPTAALEAAEILRDLVRRVAEAGRLRVGEERAAQMIHAAGMGVVLSLI
                     AAEPGDRDLALSEATREVILAAVTTDAAPDTETGRDLASSRAVALKAVLPETAAFTPP
                     ERALLSDWLDRLANHP"
     gene            complement(1965..3293)
                     /locus_tag="VFJ72_12270"
     CDS             complement(1965..3293)
                     /locus_tag="VFJ72_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7480281.1"
                     /translation="MTETIQAQAQAVAGARMHEFSRIKEVVSGWSSVRQYLRGGDQGQ
                     IVPVVIPKDRRRYGWLFLIGFALYLVGFAVFSGNAALAVLAVLGAVVFLALAALWLWR
                     GAIVEIEQGTTGVLSRYGRIEGTLSPGRHYLWRPWDKVEFVVDTATEIPYLAPVAVCP
                     TQENVPLKAIEFFLKFRITEPVAFVRTIGASNFDLVLSSAVQDAIRQRSRQVSTEKAY
                     DLRGSDVGDMQEYLNRQLASYGVRILGANIPDVQLPDQYQTHLATRERVAKELAAYER
                     EWELTRKQRIDTLLMEIERSKKDREARLIEVNEARNRARQDVARMLEEKETEAQRVRL
                     EIETRGRAALTEAENEATALRHLGQTHRDNRAVVQYELARRRVEVGEKLMQRAPRPFL
                     MRGEGSEQSALSILILAQLLPQLTAQNGGASNGAQNAIRERVRDLVDGEG"
     gene            complement(3290..4960)
                     /locus_tag="VFJ72_12275"
     CDS             complement(3290..4960)
                     /locus_tag="VFJ72_12275"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET7480282.1"
                     /translation="MVERLGERSRDFARGMARDDETEQFRIDARDFLSARERGGTTGT
                     RIEQQEAALSDAAEIVNRSFPALDPSGQFINAISPVVIPNRQSLFWRLMPIGMMVVFF
                     VVGALVTLTIVGVAGGGIGLALFGPHLWLLLVLLAVFSWWRQSVVMVPDGCKAVITRF
                     GKLEEVVEAGRKLLFNPWKQVSYIVNTTREYPYNAPIRQAPTASRVNASVDLFLQFRI
                     EDPAEFIFTVGGVKGFSDKLENAISEVTRALIYEQRAEDIYDLVGESTQKFLDSLNEQ
                     FLPAVRFTNANITHAEPSSQEYRMDLAAPEMVRVAKEAYTYEYELRLKKEQDEGALKG
                     ELASLRETLSAISAEIATYQAQIDTARERETNRANARARQRMVEAESEANANAALLEA
                     QALDIRAVSSAGAPEILEYRFQQDVLEKIAAVADRLPQVVQIGAGGADGEEAVDFLQI
                     ARRMIGTGGDSLFTSEDMQAIRNRMQEIQARIREREREIEGLSTERAASDVPEDEPNP
                     QQAAEAETADRLEEIRQSVTDEAVRERVERIGHDGDGQPTGDGREESR"
     gene            complement(5059..>6993)
                     /locus_tag="VFJ72_12280"
     CDS             complement(5059..>6993)
                     /locus_tag="VFJ72_12280"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HET7480283.1"
                     /translation="DIGFDASINPYRGCSHGCVYCMSGDTPVLMADGTARRLEDVRVG
                     DEVYGTFRRGAYRRYTKTQVLDHWSVKKPAYRITLADGTVLVAGGDHRFLTERGWKFV
                     TGAMCGDGQRPYLTLNNKLIGTGAFAEQPVKGNDYEQGYLCGIVRGDGYLASYHYERV
                     GRLHDNQHQFRLALTDEEALRRADQYLLGLQIATRAFVFQEATGGAKLMRAIRTSVSA
                     SARASVERIREIVAWPLLPSDSWCKGFLAGIFDAEGGYTGSPRIFNTDREIIAHVVLG
                     LERLGFEYAVEQAGKNRSKPMQGIRLLGGLKEHLRFFHTVEPAITRKRDIEGLAIKSD
                     ADLRVVSIEPLGVRKLFDITTGTGDFIANGVISHNCYARPTHEYLGLSAGLDFESKVL
                     VKEDAPELLRRQLSSKRWEPKVLSMSGVTDPYQPVEKKLGITRGCLEVLAEFRNPVAI
                     ITKNHLVTRDIDLLSELARHEAAVVAVSLTTLDDDLRRVMEPRTSRPVRRLAAIEKLA
                     AAGIPVGVMTAPVIPGLTDHELPNLISAAAEAGASFAAFVPVRLPGAVQPLFEDWLEK
                     RFPERKEKVLNRIRSMRDGRLNDPEFGTRMRGEGIFAEHIARLFEISCRRAGIERGRL
                     PKLSTAAFRRPRKGQPSLFD"
BASE COUNT         1157 a         2386 c         2181 g         1269 t
ORIGIN      
        1 ctggaccagg cgctcagaag ggccatcgag ccgcactacc cggagctaaa agagatccac
       61 ctctccaact acaaggtccg catcctggac gagcaccgcg ccaccgccgc caccacccgc
      121 gtcctcatag actcgaccga cggcaagcgc gtctggggcg ccgtcggcgt cggcgagaac
      181 atcatcgaag cctcctggca ggctctcgtt gacgggctgg agtacggcgt gaacgggacg
      241 aagaaaggct agcgcccttc cgcccgcccg tcatcggggc gggcggcttc gtcgaggatg
      301 ccctgtgcgg ctctacagat gctgcccacc cgacgcctcg atgcgctgcg cgttcaccca
      361 gcccatctcg ggcgagagaa gcgacgccac aaccccgccg atgtcatcag gcagcccggc
      421 gcgacccagc gcagtgtcgg aagcgaccat ctcgttcatc tccgggttgt cgcgcaccgc
      481 gccgcccgag aagtccgtct cgatggcccc cggcgcgatg gtgttcgcag cgatccgacg
      541 cggcccgagc tcgagcgcca tgtacctcgt catcacctcc acagcgccct tggccgaagc
      601 gtacgccgcg tgtcccggca gggcaaagcg cgccaggccg gtggacacgt tcacgatgcg
      661 cccgccgtcg tccatcaacg gcaacagcct ctgggtcagg aagaacggcc ccttgacgtg
      721 aatgtcaaag agctggtcga actgctcctc ggtggtttcg gcgatgctgg catgaacacc
      781 tatcccagcg ttgttgacca ggaagttgaa acgatcaaca ctccaatctc ctaatacctc
      841 ccgcacctcc tcgacgaagg cgtcgaacgt ggaggcgtcg cccacgtcca gttgcagagc
      901 ggcggcgcgg cgacccatcc cttccaactc gtccaccaac tcgtcggcgg cggccctgtt
      961 gctctggtac gtgaagatca cgtccgtgcc gcgccccgcg atgctccgca ccatgttctt
     1021 gccaagcccg cggcttccac ccgtgatgat cgccaccctg tcctgcgtgg ctccgttttg
     1081 cacgtccatc cttgcctcca gtctccgacc cttcgagccg gaatagctaa catcgctaac
     1141 attatcacca ctccatcgat aacacaaccg atgctaacaa aattatgtta tcgtatgtgt
     1201 catgaatgag acaatggtga gcaagagagc tagtacgagg gataggatag tgcgcgccgc
     1261 ggcgcggcta ttggcggagg gaggccggga ggcggtgtca acgcgggcgg ttgcggcggc
     1321 tgccggggtg caggcgccca cgatctaccg ccagttcggc gacatgcggg gtctgctcga
     1381 cgaggtggca agcttcgggt tctcggtgta tctacgggac aagacgacgc gggaaagggc
     1441 ggaggacccg gtggaggacc tgcgtcgggg ttgggacctg cacgttgggt tcgggctcgc
     1501 caacccggct ttctacacgc tcatgtacgg ggaccccagg ccgagggccg agccaaccgc
     1561 ggcgctcgaa gccgccgaga tactgcgaga tttggtcagg cgcgtcgccg aagccggccg
     1621 tctacgcgtc ggggaggaac gcgccgcgca aatgatccac gccgccggga tgggcgtcgt
     1681 cctgtccctc atcgccgccg agcccggaga ccgcgacctc gcgctgtcgg aggcgacccg
     1741 cgaggtgatc ctggccgcgg tgaccacgga cgcggcaccg gatacggaga ccggccgcga
     1801 tcttgcctcc agccgcgccg tcgccctgaa ggccgtgctg cccgaaaccg ccgcgttcac
     1861 gcccccggag agagccctgc tctcggactg gctcgacagg ctcgccaacc atccctgaag
     1921 cctgacgcct ggcgccaagg tttgcagaag gactgcaaac ctctctagcc ttcgccgtct
     1981 acaagatcac gcaccctctc gcgtatggcg ttttgcgcgc cattcgacgc gccaccgttc
     2041 tgggccgtta gctgcggtag gagttgggcg aggatgagga tcgagagcgc ggactgctcg
     2101 ctgccctcac cacgcatgag gaatggacgc ggggcgcgct gcatgagctt ctcgccgacc
     2161 tcgacccggc ggcgggcgag ctcgtactgg actacggccc ggttgtcgcg gtgggtctgg
     2221 ccgaggtgcc gcagggcggt cgcctcgttc tcggcctcgg tgagggcggc gcggccgcgg
     2281 gtctctatct ccaggcgcac gcgctgggcc tcggtctctt tctcttcgag catccgggct
     2341 acgtcctggc gggcgcggtt gcgggcttcg tttacctcga tcaggcgcgc ctcgcggtct
     2401 ttcttggagc gttctatctc catcaagagc gtgtcgatgc gctgtttgcg ggtcagctcc
     2461 cattcgcgct cgtaggcggc gagttccttg gcgacgcgct cgcgggttgc tagatgggtc
     2521 tggtactggt ccgggagctg cacgtcgggg atgttggccc ccaggatgcg gacgccgtag
     2581 cttgcgagct ggcggttcag gtactcctgc atgtccccca cgtccgagcc gcgcaggtca
     2641 taggccttct ccgtgctcac ctggcggctc cgctgccgga tggcgtcctg caccgcgctg
     2701 gataggacga ggtcgaagtt ggaggcgccg atggtccgca cgaatgccac gggctcggtg
     2761 atgcggaact tgaggaagaa ctcgatggcc ttgaggggca cgttctcctg cgtcggacag
     2821 acggcgacgg gggcgaggta gggaatctcg gtcgccgtgt ccacgacgaa ctctacctta
     2881 tcccacggac gccacaggta gtgccgcccc ggcgagagcg taccttcgat cctcccgtag
     2941 cgcgacagca cgccggtcgt cccctgctct atctccacga tggccccccg ccacagccag
     3001 agcgcggcga gcgccaggaa gaccaccgct cccaggaccg ccagcaccgc gagcgccgcg
     3061 ttcccggaga agaccgcgaa gccaaccagg tacagcgcga agccgatcag gaacagccag
     3121 ccgtagcgcc tgcggtcctt cgggatgacg acgggtacga tctgcccctg gtcgccaccc
     3181 ctcagatact gccgcacgct ggaccagccg gagacgacct cctttatcct ggagaactcg
     3241 tgcatccggg cgccggccac cgcttgcgcc tgagcctgta tcgtctcggt catcggctct
     3301 cctccctgcc gtcgccagta ggctgtccgt ctccgtcgtg gccgattcgt tcgacccgtt
     3361 cgcgcacggc ctcgtcggtc acggactgac ggatctcctc taacctgtcc gccgtctccg
     3421 cctcggcggc ctgttgggga ttgggctcgt cttcgggaac gtcgctggcg gcgcgttcgg
     3481 tggagaggcc ctcgatctcc cgctcgcgct cgcggatgcg ggcctggatc tcttgcatcc
     3541 ggttccttat cgcctgcatg tcctcggagg tgaacagcga atctccgccc gtcccgatca
     3601 tacgccgcgc tatctggagg aaatccaccg cctcctcgcc gtcggctcca cccgcgccta
     3661 tctggaccac ctgcggcagc ctgtccgcca ccgccgcgat cttctccagc acgtcttgct
     3721 ggaaacggta ctccagtatc tccggcgctc ccgcgctgct gaccgcccga atatccagag
     3781 cctgcgcttc gagcagcgcc gcgttggcgt tcgcctcgct ctcggcctca accatcctct
     3841 gccgggcgcg ggcgttggcc cggttcgtct cgcgctcgcg agcggtgtcg atctgggcct
     3901 ggtaggtggc tatctccgcg ctgatcgccg agagcgtctc ccgcaaggac gcaagctctc
     3961 ccttcagcgc accctcgtcc tgctccttct tcagccgcag ctcgtactcg taggtgtacg
     4021 cctccttcgc cacacgcacc atctccgggg ccgccaggtc catccggtac tcctggctgg
     4081 aaggctcggc gtgcgtgatg ttcgcgttcg tgaagcgtac cgccgggagg aactgctcgt
     4141 tcagggagtc gaggaacttc tgggtgctct cgccgacgag gtcgtagatg tcctcggcgc
     4201 gctgctcgta gataagcgcc cgcgtaacct cgctgatggc gttctccagc ttgtccgaga
     4261 accccttgac cccgcccacc gtgaagatga actccgccgg gtcctcgatc ctgaactgca
     4321 agaacaggtc aacgctcgcg ttcacccggc tcgccgtcgg ggcctgccgg atgggggcgt
     4381 tgtacgggta ctcccgcgtg gtgttgacga tgtagctgac ctgtttccac gggttgaaga
     4441 ggagcttgcg gcccgcctcg acgacctcct ccagcttccc gaagcgcgtt atgaccgcct
     4501 tgcatccgtc cgggaccatc acgacgctct ggcgccacca ggagaagacg gcgagcagca
     4561 ccaggagcag ccacaggtgc ggcccgaaga gcgcgagacc gatcccacct ccagcgacgc
     4621 cgacaatggt gagtgtaacc agcgcgccca cgacaaagaa cacgaccatc atccctatag
     4681 gcatcagacg ccagaacaga ctctgcctgt tcgggatgac cacgggcgag atggcgttga
     4741 tgaactggcc cgaggggtcg agcgccggga agctgcggtt gacgatctcc gccgcgtccg
     4801 agagcgccgc ctcctgctgc tctatgcgcg tacccgtcgt accgccgcgc tcccgcgccg
     4861 agaggaaatc cctcgcgtct atacggaact gctcggtttc gtcgtcccga gccatccccc
     4921 tcgcaaaatc tcgcgacctc tccccgagcc tctccaccat acgaccctcc gaaacctcag
     4981 aactacctcg aacccatcta ccattccata ctacacagtg tgccctgcaa cacaacccgg
     5041 accagcgtcc ggttcgcctc agtcgaagag gctcggctga cctttgcggg gccggcggaa
     5101 cgcggcggtg gagagcttcg gcaagcgtcc gcgttcgatc ccggcgcggc ggcagcttat
     5161 ctcgaatagc cgcgcgatgt gctccgcgaa gataccctct cccctcatgc gcgttccgaa
     5221 ctccgggtcg ttgagcctgc cgtcgcgcat ggagcggata cggttcagaa ctttttcctt
     5281 gcgctcgggg aagcgcttct ccagccagtc ctcgaagaga ggctgtacgg cgcccggcag
     5341 ccgcaccggg acgaaagcgg cgaagctcgc ccccgcttcg gccgcggcgg agatgaggtt
     5401 cggaagctca tgatcggtga ggccgggtat gacaggcgcc gtcatcaccc cgaccgggat
     5461 tccggccgcc gcaagcttct cgatagcggc gagcctccta acgggccggg aggtgcgcgg
     5521 ctccatcacc cggcgcaggt cgtcgtccag ggtcgtaagt gagactgcga ccacggcggc
     5581 ctcgtggcgg gcaagctccg agagcaagtc gatgtccctg gtaacaaggt ggttcttggt
     5641 gatgatggcg acggggtttc ggaactccgc gaggacttcg aggcagccgc gggtgatccc
     5701 gagcttcttc tcgaccggct ggtaggggtc cgtgacgccg ctcattgaga ggaccttcgg
     5761 ctcccatcgc ttcgatgaga gctgtctccg caacagttcg ggcgcgtctt cctttacgag
     5821 gaccttgctc tcgaagtcca ggcccgcaga gagtccgagg tactcgtgag tcggacgagc
     5881 gtagcagttg tggctgatga cgccgttggc gatgaagtcg cccgtgccgg tggtgatgtc
     5941 gaagagcttc ctgacgccga gcggttctat cgagacgacg cgcaggtcgg catcgctctt
     6001 gatggctaga ccttcaatat cgcgcttgcg cgtgatcgcc ggttctaccg tgtggaagaa
     6061 gcgcaggtgc tccttcaggc cgccgagcag ccggatcccc tgcatgggct tcgagcggtt
     6121 cttgcctgcc tgctcgaccg cgtactcgaa gcccagtctt tcgagcccga ggactacgtg
     6181 agcgatgatc tcccggtccg tgttgaagat gcgcggagag ccagtgtagc cgccctccgc
     6241 gtcgaagatg cccgccagaa agcctttgca ccaggagtcg gagggtagca aaggccacgc
     6301 aacgatctcc cggatgcgct cgacgctcgc acgggcgctc gcactgacgc tcgtgcgtat
     6361 cgcccgcatg agcttcgcgc cgccggtagc ctcctgaaat acaaaagcgc gcgtcgctat
     6421 ctgaaggccc agaaggtatt ggtccgcgcg tcggagagct tcttcgtcgg ttagcgcgag
     6481 ccggaactgg tgctggttat cgtgtagccg ccccacccgc tcgtaatggt acgaagcgag
     6541 atatccgtct ccccggacga tcccgcacag atagccttgc tcgtagtcgt tgcccttgac
     6601 cggctgctct gcgaaagccc cggtccctat cagcttgttg ttgagtgtca ggtatggtct
     6661 ctgcccatca ccgcacatcg cgccggtcac gaacttccaa cctcgctccg tcaggaaacg
     6721 atgatcgccg ccggcgacga gcaccgtacc gtcggccagc gttatgcggt acgccggttt
     6781 cttcacgctc cagtgatcga gcacctgcgt cttcgtgtaa cggcggtagg ctccgcgcct
     6841 gaaagtgccg taaacctcat ccccaacccg cacgtcttcg agccgcctgg cagtaccgtc
     6901 cgccatcaag accggcgtgt cgccggacat acagtaaacg cacccatgag aacatccacg
     6961 gtacgggttg atgctggcgt cgaagccgat gtc
//
LOCUS       DASSMB010000204        12102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_170974, whole genome shotgun sequence.
ACCESSION   DASSMB010000204 DASSMB010000000
VERSION     DASSMB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170974"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VFJ72_12285"
     CDS             <1..1290
                     /locus_tag="VFJ72_12285"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7480284.1"
                     /translation="TTKSFTALATMQLVEAGKVDLDSPVQRYLPWFRAANKEASSRIT
                     VRDLLNQTSGLPTRETAAQLTKNDNSDGALENQVRDLKNTQLTAPVGTQFQYSNLNFD
                     VLGLIVQTVSGQPYEEYVQQNIFEPLEMSRSYTSPEEARENGRAMGHRYWFGRPFAYE
                     MPYNRGHFPAGFINASAEDVTHYLIAQLNDGRYEGERILSPSGIAEMHRPAVETGNEG
                     TSYGMGWFVGNTNGVPTVYHSGDTASFHADLVMAPENEWGVVVLTNGQNGMSNARIAG
                     IATGVTSLLVGKEPPVAASNSARPTLLVWVAAVLAIQILGIVRSAILLRRWYRDPARR
                     PRGWFRVAIRVVPFFLASFLWAMVAISVLPFFAGAPLLALPMFVPDLGYPLLLSIGIA
                     LGWGLLRPVLVLRVLRSRRKPPRDVDTATERTVTATT"
     gene            1338..2498
                     /locus_tag="VFJ72_12290"
     CDS             1338..2498
                     /locus_tag="VFJ72_12290"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HET7480285.1"
                     /translation="MRTEEQNATGGVEREPTVQGVSSEWQGGQYSLAKILGIWAAAAF
                     PIGALGWVATPWLSQYIHSRDPFIDSLLICFNLWMVALVLILVQRERRSLARARDWLV
                     AVLPVDRFGASRVRHKAVRHVIASHSEPDTTSLDWKRMRRATLLVVTAAITLLLAGCG
                     GNGPGDLRTESRAVDPHGAKSVSTHLEMGFGTLNVEGGTHKLMEADFAYGNDDWKPRI
                     EYGVNGEKGTLKVWQGSDNGNVPFSGLKDVRNDWTLHLNDRVPEDLRVEMGAGKSDLR
                     LGSISLKSLDVQMGAGAANVDLTGDWDHDLNVDIEGGAGYATLRLPTKVGARVNVRGG
                     FGRISADGLRKEGNAYVNDAYGNSDVTLNVDFEGGVGQVNLEAVEQGEAGQE"
     gene            2839..3024
                     /locus_tag="VFJ72_12295"
     CDS             2839..3024
                     /locus_tag="VFJ72_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480286.1"
                     /translation="MAALAWVVAPLVAEGLDGSVALARVLLVGLTGGLIWQLVLVMLL
                     VYREQSSLRWSILKDAL"
     gene            3193..3501
                     /locus_tag="VFJ72_12300"
     CDS             3193..3501
                     /locus_tag="VFJ72_12300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7480287.1"
                     /translation="MSGNWVWYALIVVLSVFNTFLGEELLFRGLLLPRMRGAFGRWDW
                     VANGVLFAGYHLHMPWTIPATLLDTFALYYPSRRYRSALIGILVHSSQQVMILTLTLK
                     "
     gene            3545..3670
                     /locus_tag="VFJ72_12305"
     CDS             3545..3670
                     /locus_tag="VFJ72_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480288.1"
                     /translation="MSFYEFRDGDTSMGAGRIGSTGWRSSIQDAERLRYLPEKEE"
     gene            3899..4681
                     /locus_tag="VFJ72_12310"
     CDS             3899..4681
                     /locus_tag="VFJ72_12310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7480289.1"
                     /translation="MTAYEPNESLNPMQGTLARESVDDQHPLGKSVALHLIPGVALLA
                     SYILLTPLVVQMRFPTIFAGSLSILFVLAPLELGYLLYLGRRRNGRLSLAGIVRYREP
                     MHIWQYALFVPLLVVWYSAVISAWQGIMPALAGALSWLPDWALNPLPADDPSAQYAAP
                     ILLTAAIFRVACTGIIAPVIEELYFRGYLLPRIDRLGVWAVVIGAVLFSFQHLWTPLL
                     DPGRVIAWLPAVYLVWRKRNIYLGIIVHLALNLAGVILPLMS"
     gene            complement(4715..5461)
                     /locus_tag="VFJ72_12315"
     CDS             complement(4715..5461)
                     /locus_tag="VFJ72_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET7480290.1"
                     /translation="MKLTIHHLYADMMNLYGDRGNVISIKKRCEWRGIPVEVVDVGLR
                     EKVQPTGCDIFLFGGGQDREQALLAEDLSGSKGADLRAIVEEGSVVLGVCGGYQLMGH
                     SYETPEGEKLPGVGIFDLTTEPRKPDEPRLIGNVLVCVRAPETGEVREIVGFENHGGR
                     TYLGDTEPLGEVVVGFGNNSKDGTEGARRLNAYGTYLHGSLLPKNPWLTDQLILSALR
                     QKDDSFELEPLDDAAEQSAFASVLGRIAGR"
     gene            5588..5797
                     /locus_tag="VFJ72_12320"
     CDS             5588..5797
                     /locus_tag="VFJ72_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480291.1"
                     /translation="MRDPGLAAVLSLIIPGVGQFYNGRILAGILWLIITPGLWIGTGG
                     LLGWVAHLIAAYTAYSYARDNPVRD"
     gene            complement(5819..7237)
                     /locus_tag="VFJ72_12325"
     CDS             complement(5819..7237)
                     /locus_tag="VFJ72_12325"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HET7480292.1"
                     /translation="MNLALDPRLSAAVLAARSAQLASRTLKTGGGSTVPGVVARRVDP
                     KVLTKLSRRLPMGSAAITGTNGKTTTTRMVSKILQTAGIKAVNNSTGANLVTGVTAAL
                     VSDSGVSGRPASEMGLFEVDEASVPKVAAEADLKLLAVLNLFRDQLDRYGELAYTGKV
                     IASAFESLPPDGAVVLNADDPLVASLGRQARRPVFYGVDEPALDTGRLQHVADSKDCP
                     VCGTALDYEAVYMGHVGVYGCGNCGFSRPELQYRASRVRFDGARGTEFLLNTPKGGTA
                     VRIELPGLYNVYNAVAAAAVAGESGVGLGEIARGLREFGGAFGRVERVKAGDREAFLL
                     LIKNPVGFNEILRTFVTSADARNVLIAINDNDADGRDVSWLWDVDFEMLADARAKGST
                     NALPPFMVSGIRAEDMAVRLKYAELPVGSVVPDRKRAIREALAATPPGETLYVLPTYT
                     AMLEIRQTLSEMGYTHPFWEDK"
     gene            7381..8358
                     /locus_tag="VFJ72_12330"
     CDS             7381..8358
                     /locus_tag="VFJ72_12330"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7480293.1"
                     /translation="MEIFENLSASSKLESGPLDAYSRAVRYVTEKLEPAVISVGIPDG
                     RGGGSGVILGSDGSTATAVTNSHVVRGLRSRRARNSTGGIQITLPDGTSAPAEVLGDD
                     PPSDLAVVRFAHEGEVTVATLGEAENLVVGQLVVAIGNPLGFQRSVTAGVVSALGRSI
                     RGESGRPIENVIQTDAAINPGNSGGPLADASGRVVGINTAIIGGAQGIGFAVPVSAAF
                     RRVVFALVTEGRVRRAYLGVMVASRPTKNGSGGGAGVESVAPNSPAERAGVKPKDLIL
                     GLGENTVRSTDDLLALLDESAIGNDTPIKILRGNRELTLTVRPREQPAE"
     gene            8406..8642
                     /locus_tag="VFJ72_12335"
     CDS             8406..8642
                     /locus_tag="VFJ72_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HET7480294.1"
                     /translation="MPDVTLNVRGLSNAGETEKLERALTRLDSVQLVNVDHEKSLVAV
                     SYEGGASELEEIESAIRDAGYDYTPSPGAENVGG"
     gene            complement(8670..9404)
                     /locus_tag="VFJ72_12340"
     CDS             complement(8670..9404)
                     /locus_tag="VFJ72_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7480295.1"
                     /translation="MDFFSGEILAGLVSVLVVNLILSGDNAVVIAMAARQLEGANRRK
                     AIFWGAGGAVVLRLIFAAIVTFLLQIPFLQVVGGLLLIWIAWQLVQDDHDDEGKVQAG
                     QSTWEAVRIIIIADAVMSLDNVIALVQAARIDGDVDFILLGVGLITTIPLVIFGAALL
                     TSLLDRFPILVYLGAGLLIYLAVEMFFADVFLHHYLKPYEGIEWIIGVTAAAIFMAVA
                     WMAARRSDRRDRMPEDVRKSAGTSKD"
     gene            complement(9515..10531)
                     /locus_tag="VFJ72_12345"
     CDS             complement(9515..10531)
                     /locus_tag="VFJ72_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480296.1"
                     /translation="MSLIELVLALAAILAAAALFTNAIEILGERLNLGEGAVGSVLAA
                     VGTALPETMIPLIAIFGALIFGGDAAAAGEIGVGAILGAPFLLATLAMFVAGVAAYGY
                     RRRRGSGREITMERATAIRDLGFFLVFYTIAAGVGVLRLPFLLKLLVVVVLIAAYVLY
                     VLRTLKAEGGTEGEAPSRLTLWPKGSAPTWAVVAQVVGSLVVMAAGAHFFVDAVEHGS
                     KALGIPAGLIALILAPLATELPEKVNSVLWLKNDKDTLALGNITGAMVFQSTIPVSLG
                     IAFTPWVLEPLNLLSVVLALISGGLVFYMLRRSKPIQIPYLLAGGALYGVFVIAAVFN
                     VVFL"
     gene            10622..11452
                     /gene="larB"
                     /locus_tag="VFJ72_12350"
     CDS             10622..11452
                     /gene="larB"
                     /locus_tag="VFJ72_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET7480297.1"
                     /translation="MEKLEEILKAVASGDISPSAAASRLKTMEVRYLDGYAVLDAGRA
                     TRKGVPEIVYARGKTPEQVAGICAALLDTAERVLVSAAAPEHEAAIRRALPETPLRES
                     GRAIVLGSGVTTPTGGRVAAFCAGTSDLPALGEAVAVVEEMGVEVARFHDVGVAGIHR
                     LAGPLEELASFDPDCLIVAAGMEGALPSVISGLVAVPVIGLPTSTGYGLGGDGTAAIL
                     GMLQTCSPGLSVVNVDNGVGAGTTAAMIANRAARGRSARGYTANSTPGPLENGASGQG
                     "
     gene            11462..12019
                     /gene="thpR"
                     /locus_tag="VFJ72_12355"
     CDS             11462..12019
                     /gene="thpR"
                     /locus_tag="VFJ72_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7480298.1"
                     /translation="MAVRSFVAIFPPRGIQEALHRSARGLPIEGAIRQVRPENIHLTI
                     KFLGDVQPESLPHIGEALAAVCERHGPFHIDLSGFGAFPSERRGRILWAGVDGGSARL
                     ISLSEDVEKTLEPLDFEREPRAFRPHLTLGRARGRSFVLGEAATAERLAFRAERLTLV
                     QSVLREDGARYEPIGDYPLGGTKPG"
BASE COUNT         2223 a         3896 c         3913 g         2070 t
ORIGIN      
        1 accaccaagt cgttcaccgc cttggcgacc atgcaactgg tcgaggcggg gaaggtggat
       61 ctcgactcgc ccgtgcaacg ctaccttccc tggttccgcg cggccaacaa agaagcttcc
      121 tcgcgcatca ccgtgcgcga cctcctgaac cagaccagcg gtctgccgac gcgggagacg
      181 gcggcccagc tcacgaagaa cgacaacagc gatggcgcgc tagagaacca ggtgcgcgac
      241 cttaaaaaca cccagctcac ggctccggtc ggcacgcagt tccagtacag caacctcaac
      301 ttcgacgtcc tggggctcat cgtgcagacg gtctccggcc agccctacga agaatacgtg
      361 cagcagaaca tctttgaacc gcttgagatg agccgctcct acacatctcc tgaagaggcc
      421 cgcgagaacg gacgggcgat gggccaccgt tactggttcg ggagaccgtt cgcctacgag
      481 atgccgtaca accgcggcca cttcccggcg ggcttcatca acgccagcgc ggaagacgtg
      541 acgcactacc tgatcgccca gctaaacgac ggacgttacg aaggcgaacg gatcctctcg
      601 ccgtccggga tcgccgagat gcacaggccc gcggtcgaaa ccggcaatga aggaacgtct
      661 tacggcatgg gatggttcgt ggggaatacc aacggcgtgc cgaccgtcta ccacagcggc
      721 gacacggcca gcttccacgc cgacctcgtc atggcgcccg agaacgagtg gggcgtcgtc
      781 gtactcacga acggccagaa cggcatgagc aacgcgcgca tcgccggtat cgccaccggc
      841 gtgaccagcc tgctcgtggg caaggagccg cccgtggcgg cgtctaacag cgcccggccc
      901 accctcctcg tatgggttgc ggcggtgctc gccatccaga tactcgggat cgtccggtcg
      961 gcgatattgc tgcggcgctg gtatcgggac ccggcgcggc gtccgcgcgg gtggttccgc
     1021 gtcgcgatcc gcgtcgtccc gttcttcctg gcgagcttcc tgtgggcgat ggtcgccatc
     1081 tccgtcctgc ccttcttcgc cggagccccc ttgctcgccc tgccgatgtt cgtcccggac
     1141 ctgggctacc cgttgctgct cagcatcggg atagcgctcg gctgggggct cttgaggccc
     1201 gtgctggtgc tccgggtgct gcgcagcagg cggaagccac ccagagacgt agacacggcc
     1261 acggaaagga cggtaacggc cacgacctga gcaccaataa ccctggcaga agcacgattt
     1321 cgcaaggagg acggaagatg aggacggaag agcaaaacgc aaccggcgga gttgaaagag
     1381 aacccaccgt acaaggggtt tcgagcgagt ggcaaggcgg gcagtactcc ctggcgaaga
     1441 tcctgggcat ctgggctgcc gcggcctttc cgattggcgc cctcggctgg gtcgccacgc
     1501 cctggctcag ccagtacatc catagtcgag acccgttcat agactcgctg ctgatctgct
     1561 tcaacctctg gatggtcgcg ctggtcctga tcctggtcca gcgggagcgg agatcgctgg
     1621 cccgggcgcg cgactggctc gtggctgtcc ttccagttga tcggttcggc gcttctcggg
     1681 ttcgccacaa ggcggtgagg catgtcatag cttcgcatag cgagccggac acgacttctc
     1741 tggattggaa aagaatgaga cgagcaacgc tcctcgtggt gacagctgcg ataacgctcc
     1801 tcctcgcggg ctgtggggga aacgggccag gcgacttgcg gaccgagtcc cgagcggttg
     1861 atccgcatgg agccaagtcc gtgagtacgc atctcgagat gggcttcggc actctgaacg
     1921 tggaaggcgg aacccacaag ctgatggagg cagactttgc ctatggtaac gacgactgga
     1981 agcccaggat cgagtacggc gtcaacggcg aaaagggaac gctcaaggtc tggcagggct
     2041 cggacaacgg gaacgtgcct ttctccggtc tcaaagatgt ccgcaacgac tggaccctcc
     2101 acctgaacga ccgcgtacca gaagatctga gggtcgagat gggcgctggc aagagcgacc
     2161 tccggttagg aagcatatct ttgaagagcc tcgatgtgca gatgggagcg ggcgcggcca
     2221 acgtggatct cacaggcgac tgggaccacg acctgaacgt cgacatagag ggcggagccg
     2281 gatacgccac cttgcggctg cccaccaaag tgggggcgcg cgtaaacgtc aggggcgggt
     2341 tcggccggat cagcgctgac ggcctgagga aggaaggcaa cgcctacgtg aacgatgcct
     2401 acggcaactc cgacgtgacg ctgaacgtgg atttcgaggg cggcgtcggg caggtcaacc
     2461 tggaagcagt cgagcagggc gaggcgggtc aagaatgatg aactccagta cccaaggaga
     2521 accgataaag gtaagagcgg aagtgaaaca tacggatcgc ttgcggtggg gttggtgggt
     2581 ggcatggggc ttcattgctc atccagcggt ccttgcgacc gctcgctcag acgcagcgcc
     2641 agcattcagc gacagagaca acttgattct ggcagaagca agacttagga aggaggatca
     2701 acaaaggtgg gggggaagga cagatcgtac gcggcagagc cgtaggagac tccatggcag
     2761 aaggtgtgcc gagcaagagc cttggcgagc agtactctcg caaagattct agacgtctga
     2821 gccgctgcgg cgctgccgat ggcagcactg gcctgggtcg tggccccgtt ggtcgcagaa
     2881 gggctcgacg gctccgtagc cctggcgcgc gtccttctcg tgggcctgac gggcggcctg
     2941 atctggcagt tagtgctggt gatgctcctg gtttaccggg agcaaagctc gctgcgctgg
     3001 tccatcctga aggacgccct gtagctgcga gcaccgcgca gcctccggac caggcacgtc
     3061 ggtggatggc tctggctcgt cctggttccg tgcgtgctga tcttctaggc cgaggaattc
     3121 atcccctcgt tcccatccgt agccagccac gacttcggct cgtttctcgg gtttcacgcg
     3181 ggccaggcat tcatgtccgg caactgggtc tggtacgctc tcatcgtcgt cctgagcgtg
     3241 ttcaacacct ttctcggaga ggagctgttg ttccgcggcc ttttgctgcc acggatgcgg
     3301 ggtgcgttcg ggcgctggga ctgggttgcc aacggggtgc tgttcgcggg ctaccacctg
     3361 cacatgccgt ggacgatccc ggcgaccctg ctcgacacct ttgcactgta ctacccatcg
     3421 cggcgctacc gcagcgcact gatcggaata ctcgtgcaca gctcccagca ggtcatgatc
     3481 ctcaccctga ccctcaagta gccagttcgg tctcggaggt gcacgagtct acggaaggag
     3541 caaggtgagc ttctacgagt tcagggacgg ggacacctcg atgggcgccg gtcggatagg
     3601 ttcgacgggc tggagatcat cgatccaaga cgcggagaga ctacgctatc tcccggagaa
     3661 ggaggagtaa tgatagaagg gcgcattcct atgggatttt cacctaagtt cataacacgc
     3721 tctagccatc ggcctgtctc tgagactgga acgggcgcaa tggggtcctg tcaaacaacg
     3781 tagtcgaaca cgaagcgcaa cgatccacat gaacggggcc caacgaacac aacccggccc
     3841 tggcaggagc gagacgtcgg aaggaggacc aacgaagatg aacccagagg agacagaagt
     3901 gactgcttac gaacccaacg agagcctgaa tccgatgcaa ggtaccctgg cgcgggagtc
     3961 ggtggacgac cagcacccgt tgggcaagtc ggtagccctg caccttattc cgggcgtggc
     4021 cctgctcgct tcctacatcc tcttgactcc gttggtcgtg cagatgagat tccctacaat
     4081 cttcgccggc tcgctctcta tattgttcgt cctcgcgccg ctggaactgg gctacctgtt
     4141 gtacctggga cggcggcgca atggacggct ctcgctggcg ggcatcgtgc gctaccggga
     4201 accgatgcac atctggcagt atgcgctttt cgtaccgttg cttgtagtct ggtactccgc
     4261 ggtcatctcc gcgtggcaag ggatcatgcc ggcgctcgcg ggcgctctct cctggctccc
     4321 ggactgggcc ttaaaccctc taccggcgga cgatcccagc gcgcagtacg cggctcccat
     4381 actgcttacc gccgccatct tccgggtggc gtgcaccggg atcatcgctc cggtcatcga
     4441 ggagttgtac ttccggggct acctgttgcc gcgcatagac cggctcggcg tctgggcggt
     4501 ggtgatcggc gccgtgctgt tctctttcca gcacctgtgg acgcccctgc tcgatcccgg
     4561 ccgcgtcatt gcctggctcc cggcggtgta tctggtgtgg cgcaagcgca acatctacct
     4621 ggggatcatc gtgcacctcg cgctcaatct cgcaggcgtc atcctgccgc tcatgagtta
     4681 ggcaactgga cccgcatcct ccgttagaga gcttctagcg cccggctatt cgtcccaaca
     4741 cagaggcgaa cgcgctctgc tccgccgcat cgtccagcgg ctccaactcg aaggagtcat
     4801 ccttctggcg tagggcgctc aggatgagct ggtcagtgag ccaggggttc tttggtagta
     4861 gggaaccgtg caggtacgtc ccgtaggcat tgagacggcg ggctccttca gtaccgtcct
     4921 tgctgttgtt gccgaaaccg acgaccacct ccccaagcgg ctcggtgtcg cccaggtagg
     4981 tgcgtccgcc gtggttctcg aagccgacga tctcccggac ctcgccggtc tcaggggcgc
     5041 gtacgcacac gagcacgttc ccgatcaagc gcggctcgtc gggcttgcgc ggctctgtgg
     5101 tcaggtcgaa gataccgacg ccgggcagct tctctccctc gggcgtctcg taggagtggc
     5161 ccattaactg gtagccgccg cagacgccga ggaccacgct gccctcctct acgatggctc
     5221 tcaggtccgc gcccttggag ccggagaggt cttcggccag tagcgcctgc tcgcggtcct
     5281 ggccaccgcc gaagaggaag atgtcgcagc cggtcggttg gaccttctcc cggaggccca
     5341 cgtccacgac ctcgaccggg atgccgcgcc attcgcagcg cttctttatg gagatgacgt
     5401 tcccccggtc cccgtagagg ttcatcatgt ccgcgtacag gtgatggatg gtgagcttca
     5461 aagttcttcc cttcggccga acgtcatctt cctagcttac taccggcccg tccggcttgc
     5521 tgctggctgc gctcgggtaa gaacagcgtg caacccgaac agccctcaaa cgtaaggaga
     5581 tagtgagatg cgtgatccag gactagcggc ggtactcagc ctgatcatcc ccggcgtggg
     5641 ccagttctat aacggacgta tcctggccgg gatactgtgg ctcatcatca cgccgggact
     5701 ctggatcggc accggcggac ttttgggctg ggtcgcccac cttatcgccg cctacaccgc
     5761 ctactcgtac gcccgcgaca acccggtgcg cgactgatcc cggcgccctc tatggcgcct
     5821 atttatcctc ccagaacggg tgcgtgtacc ccatctcgct gagggtctgc cggatctcca
     5881 acatcgccgt atacgtcggg agcacatata gcgtctcgcc cggcggcgtc gccgcaagcg
     5941 cctcccggat ggccctcttg cggtccggta cgacagaccc gaccggaagc tccgcgtact
     6001 tcagcctcac ggccatgtcc tccgcccgga tgccgctgac catgaacggc ggcagggcat
     6061 tcgtactgcc tttcgcacgc gcatcggcga gcatctcgaa gtccacgtcc cagagccagg
     6121 agacgtcccg gccatcggcg tcgttgtcgt ttatggcgat gaggacgtta cgggcgtcgg
     6181 cgctggtgac gaaggtacgc aggatctcgt tgaacccaac cgggttcttg atgagcagca
     6241 ggaacgcctc ccggtcaccg gcctttaccc gctcgaccct cccgaaagcc ccgccgaact
     6301 cccgcaggcc ccgcgctatc tccccgagcc cgacccccga ctctccggcc acggcggcgg
     6361 cggccacggc gttgtagacg ttgtacaggc ccggaagctc tatccgaacc gccgtcccgc
     6421 ccttcggagt gttgaggagg aactcagtcc cccgcgcccc gtcgaagcgc acccggctgg
     6481 cccggtactg gagctcgggc cgcgagaagc cgcagttccc gcagccgtac accccgacgt
     6541 gccccatgta cacggcctca tagtcgagcg ccgtaccgca gaccgggcag tccttcgagt
     6601 ccgcgacgtg ctgcaaccgg cccgtatcca gcgcgggctc gtccaccccg tagaagaccg
     6661 gcctgcgcgc ctggcggccc aggctcgcga cgagcgggtc gtccgcgttc aggacgaccg
     6721 cgccatccgg cggcaggctc tcgaaggcag aggcgatgac cttgcccgtg taggccagtt
     6781 ccccgtagcg gtcgagctgg tcgcggaaga ggttcagcac ggccagaagc ttcagatccg
     6841 cctcggcggc gaccttcggc acgctcgcct cgtccacctc gaagagtccc atctccgagg
     6901 ccggtctgcc ggagacgccg gagtccgaga ccagcgcggc cgtcacgccg gtgaccaggt
     6961 tcgcgccggt ggagttattt acggccttga tgccggccgt ctggaggatc ttagagacca
     7021 tccgggtcgt ggtcgtcttg ccgttggtgc ccgttatggc cgccgagccc atcggcagac
     7081 gccgggagag cttcgtgagg accttcgggt caacgcgccg ggccaccact cccggcaccg
     7141 tggagccgcc gccggtcttc aaagtccggc tggcgagttg cgccgagcgg gcggcgagga
     7201 cggcagcgga gagacgggga tccaacgcca ggttcatggc gcggattata tccggtagcc
     7261 gcggggcctt ttgcggacgc tccccgaagg ggacttcaca ggaccgcatg ctggttcaaa
     7321 gcgcaaaaac ctggccgttc ttccagggcc aacgcaaccc ggatgcggta aaaatagcga
     7381 atggagatct tcgagaacct ttcggcaagc tccaagctgg aatcgggtcc gctcgacgcc
     7441 tactcgcggg cggtgcggta tgtgacggag aagctggagc cggccgtgat ctcggtgggc
     7501 atcccggatg ggcgcggcgg cgggagcggc gtcatcctcg gctccgacgg ctccacggct
     7561 accgccgtta ccaacagcca cgtcgtccgc ggcctccgta gccgccgcgc caggaacagc
     7621 acgggcggca tccagataac gctcccggac gggacctcgg ccccggcgga ggtgctgggc
     7681 gacgacccgc cgagcgacct cgcggtggtc cgcttcgcgc acgaaggcga ggtaacggtc
     7741 gccaccctgg gggaggcgga gaatctggtc gtgggacagc tcgtggtcgc cattggcaac
     7801 ccgctcggtt tccagcgcag cgtgacggct ggcgtggtga gcgccctggg gcgttcgatc
     7861 cggggcgaga gcggacgtcc catcgagaac gtcatccaga cggacgcggc catcaacccc
     7921 ggcaactccg gcggtcccct ggccgacgct tcggggaggg tagtcggcat caacacggcg
     7981 atcatcgggg gcgctcaggg gatcgggttc gcggtgccgg tctcggcggc gttccggcgc
     8041 gtggtcttcg cgctcgtcac cgagggccgc gttcgccgcg cctacctcgg cgtgatggtg
     8101 gcgagccggc cgacgaagaa cggttccggc ggcggggccg gagtagagag cgtcgccccg
     8161 aacagtcccg ccgagcgcgc gggcgtgaaa cctaaagacc tgatcctggg cctcggcgag
     8221 aacaccgtcc gctccacgga tgacctcctc gccctgctcg acgagtccgc catcggcaac
     8281 gacactccga taaagatcct gcgcggcaac cgcgagctta ctctaacggt tcgtcctaga
     8341 gaacagcccg ccgagtagtc cgacggagta agattagatc tccgctacag ggagggagcc
     8401 agaacatgcc ggacgttacg ctaaatgtgc ggggtctctc gaacgcggga gagacagaga
     8461 agctggagcg cgccctgacc cgcctggact ccgtgcaact cgttaacgtg gaccatgaga
     8521 agagcctcgt cgcagtctct tatgaaggcg gcgcttccga gcttgaagag atagagagcg
     8581 ccataaggga cgccggttac gactacacgc cctcacccgg cgcggagaac gtcggcggct
     8641 gacttttccg ctcccggctc tccgcggcgt cagtctttcg aggttccggc gctctttctg
     8701 acgtcttccg gcattcggtc ccgcctatcc gagcggcgtg ccgccatcca ggccacggcc
     8761 ataaatatcg cggcggccgt aacgccgatg atccactcga tgccttcgta gggtttgagg
     8821 tagtggtgca ggaacacgtc cgcgaagaac atctccacgg cgagatagat aagtagtcca
     8881 gcaccgaggt agaccaggat cgggaagcgg tcgaggaggg aggtcaggag cgcggccccg
     8941 aagataacga gggggatggt cgtgatcagc ccgacaccga ggaggataaa gtcaacgtca
     9001 ccgtctatcc tcgcggcctg gaccaacgct atcacgttgt ccagggacat cacggcgtcg
     9061 gcgatgatga tgatcctgac ggcttcccag gtgctctgcc cggcctgcac cttgccctcg
     9121 tcatcgtggt catcctggac gagttgccag gcgatccaga tcagcagtag cccgccgact
     9181 acttgaagga acgggatctg aagcagaaac gtcacgatgg cggcgaagat tagacgcagc
     9241 accacggcac cgcccgcacc ccagaatatc gccttgcggc ggttcgcgcc ttcgagctgg
     9301 cgggcagcca tcgcgatgac taccgcgttg tcgccggaga ggatcaggtt gaccaccagc
     9361 acgcttacga gcccggccaa tatctctccg ctaaagaagt ccatctaggc gcttatacct
     9421 ccggcatcgg acccggcacg actcggaccg gcccgcagat cgttcgtaac tgtttgtatc
     9481 ttatcaggaa agctacatca tttattcagt aattctatag gaatactacg ttgaagaccg
     9541 ccgcgatcac gaagacgccg tagagcgctc cgccggcgag gaggtacggt atctggatgg
     9601 gcttgctgcg ccggagcata tagaagacga ggccgccgga gatgagcgcc agcacgacgg
     9661 agaggaggtt gagcggctcc aggacccacg gcgtgaacgc gatgcccaga gagacgggaa
     9721 tggtgctctg gaagaccatc gccccggtta tgttgccgag cgcgagcgtg tccttgtcgt
     9781 tcttcagcca gagtacggag ttgaccttct ccggcagttc cgtcgccagg ggagccagga
     9841 tcagggcgat gagaccggcg gggatgccga gcgccttcga gccgtgctcc acggcgtcca
     9901 cgaagaagtg cgcgcccgcc gccatcacga ccagagagcc gaccacctgc gcgacgacgg
     9961 cccaggtagg cgccgaacct ttcggccaga gcgtgagcct ggagggcgcc tcgccctcgg
    10021 tgccgccctc ggctttcagc gtcctcaaca cgtacaagac ataggcggcg atcaggacca
    10081 ctacgacgag gagtttgagg aggaacggca ggcgcaggac ccccaccccc gcggctatcg
    10141 tgtagaagac gaggaagaac cccaggtccc tgatggccgt ggcgcgttcc atggttatct
    10201 cccgcccgct gcccctgcgc cgccggtagc cgtatgccgc cactccggcc acgaacatcg
    10261 cgagcgtcgc gagcaggaaa ggcgcgccca gtatcgcccc gactcctatc tccccggccg
    10321 ccgccgcgtc accaccgaag atcagggctc caaagatcgc gatcagcggg atcatggtct
    10381 ccggtagagc ggtcccaacg gccgccagca cgctcccgac cgccccctcc ccgaggttca
    10441 acctctcgcc aagtatctcg atggcattag tgaacagcgc ggcagcggcc agtatggcgg
    10501 ccagggccag gactaactct attaacgaca acgcatacct ccctcggaga tgccaggcac
    10561 ggaaaacgtg ctagattcta cccgctggta atccggctca agctatagag ctataggaga
    10621 gatggagaag ctcgaagaga tcctgaaagc ggtggctagc ggcgacatct cgccctcggc
    10681 ggcggcctcg cgcctgaaga ccatggaggt gcgctacctg gacgggtacg ctgtcctgga
    10741 cgcgggacgc gccacccgca agggagtccc ggagatcgtc tacgcgcgcg gcaagacacc
    10801 ggagcaggtc gccggcatct gcgccgcgct cctcgacacc gcggagcgcg tcctcgtgag
    10861 cgccgccgcc ccggagcacg aggccgccat ccggcgcgcc ctaccggaga cgcccttgcg
    10921 cgagagtgga cgcgcgatcg ttctgggttc gggcgttacc acgcccaccg gcggacgggt
    10981 tgccgccttc tgcgcgggta cttcggacct cccggcgctc ggagaggcgg tcgcagtggt
    11041 cgaggagatg ggcgtggagg tggcgcgctt ccacgacgtg ggggtggcgg gcatccaccg
    11101 gctcgcgggg cctctggaag agcttgcgag cttcgacccg gactgcctga tcgtggccgc
    11161 cggcatggag ggcgccctgc cgagcgtcat ctccgggctc gtggccgtgc ccgtgatcgg
    11221 gctaccaacc tcgacgggct acggactcgg gggcgacggg accgccgcca tcctggggat
    11281 gctccagacc tgctcccccg gcctctcggt cgtgaacgtg gacaacggcg tcggggccgg
    11341 gacgaccgcc gccatgatcg ccaaccgcgc cgcgcgtgga cgctcagctc gcggatacac
    11401 cgcgaactct actcccggcc cgctggagaa cggcgcctcc ggccagggat aggaacctgc
    11461 gatggccgtg cgctccttcg tcgccatctt cccaccgcgt gggatacagg aagcgctgca
    11521 ccgctcggcg cgcggacttc caatcgaggg agccatccgg caggtgcgtc ccgagaatat
    11581 ccacctgacg atcaagttcc tgggcgacgt tcagccggag agcctgcccc atataggaga
    11641 agctctggcc gcggtatgcg aacgacacgg tccgttccac atcgatctat ccgggttcgg
    11701 agccttcccc tcggagcgca gggggcgcat tctatgggcg ggcgtagacg gaggaagcgc
    11761 gcgtctgatc tcgctctccg aagacgtgga gaaaaccctg gagccactgg acttcgagcg
    11821 cgagcctcgc gctttccggc cgcacctgac actcggacgt gcgcgcggca gatcatttgt
    11881 tctgggagaa gccgccacgg cggagagact ggcattccgg gccgagcgcc taactctagt
    11941 tcagagcgtg ttgcgggagg acggggcgag gtatgagcct atcggagact atcccctggg
    12001 tgggacaaag ccgggctaga tcgagaccag aggcccgacg gccatgagga tgaagagtag
    12061 gatcaggatc atcacgacga tcgctatgaa gttgttcccg ct
//
LOCUS       DASSMB010000205         3096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_435888, whole genome shotgun sequence.
ACCESSION   DASSMB010000205 DASSMB010000000
VERSION     DASSMB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435888"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VFJ72_12360"
     CDS             <1..831
                     /locus_tag="VFJ72_12360"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7480299.1"
                     /translation="FFAKILYAEGVSFEALVAFRAVVGWVAVVLFVLATGGARSLRVP
                     GRDLAFLAPLGLIGIGAFYLLYFYTVRESTIGTAAILLYSSPAFVVVLAWLFLGEKLG
                     RAKIAALVLTFCGISLVAGAYDPANLEVSPAVLATGLLSGLTYGLYSIFGRPVAGRLP
                     PSVILSYALFFGGILLLAFAVPTLGTLTGLSPVSYAILLMLAVVHTTLAFALYTFGLG
                     RLGAGRSAIVATVEPVVAGALGVLLLGEAATAAKVVGALLVLAGAALAQLRTRKARVS
                     "
     gene            965..2089
                     /locus_tag="VFJ72_12365"
     CDS             965..2089
                     /locus_tag="VFJ72_12365"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7480300.1"
                     /translation="MPIEFNGSGGYTLGVEEELHIVDATTGELVPKIEEIMARLPEDL
                     CEFVSYELFQSVLEIKTSVCRNVAEAEKQLRELRSRVNSWAAACGASLAAAGTHPFSR
                     YKDQKITDQERYRKVIETLRWVAQREVIFGQHVHVAVPGPEEAIIAHNRLSEQAPLLL
                     ALSANSPFWQGMDTGFESTRVKIFETFPRAGMPPAFPDWESFEGFVDMMVESGAMDDY
                     TYCWWDVRPHPKIGTIELRILDTQTGLRPAIALTALTQCLVARALEEIGPHVPYDREL
                     ALENKWRASRHGMDAAFYDAKEKFNVPARDMARDLVEQLKPHSQDLGCESELLEVLEI
                     VEGGTGAQQQREAYKESDDFLDVVAFLIEGTRPALAGEQS"
     gene            complement(2115..2354)
                     /locus_tag="VFJ72_12370"
     CDS             complement(2115..2354)
                     /locus_tag="VFJ72_12370"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7480301.1"
                     /translation="MFTGLESPTHLLFIVLVILLFFGGKRIPELARGLGKGMREFRQG
                     LEERDEEHKTQAHAEETKKLDRDEESDARTDRERS"
     gene            complement(2485..2769)
                     /locus_tag="VFJ72_12375"
     CDS             complement(2485..2769)
                     /locus_tag="VFJ72_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET7480302.1"
                     /translation="MVTAVVLITTEGERVQEAAQAMLGIEGVTEAYSVTGPYDLVVMV
                     RIPDFERLAEIVPEKIAQVPGVARTETMVAFRTYSNYDLDRAWSIGFEDE"
     gene            complement(2779..>3096)
                     /locus_tag="VFJ72_12380"
     CDS             complement(2779..>3096)
                     /locus_tag="VFJ72_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480303.1"
                     /translation="LYQGGNVVLTGDSETVFTVTVTNGGEVAEQAVPVEVILNTKAER
                     QSQKVTVESIAPNSGTQTVKVSGFRPGEYDETADVTVEVGPVKYEDYTDNNTLTGTVT
                     FGL"
BASE COUNT          509 a         1029 c          962 g          596 t
ORIGIN      
        1 ttcttcgcga agatactcta cgccgagggc gtctcgttcg aggcgctggt ggcgttccgg
       61 gcggtcgtcg ggtgggtggc cgtggtcctc ttcgtcctgg cgaccggcgg agcgcggagc
      121 ctgcgagtgc cgggcagaga cctcgccttc ctggcgccgc tggggttgat cgggatcgga
      181 gctttctatc tactctactt ctacacggtg cgagagagca cgatcgggac ggcggcgatc
      241 ctgctctaca gttcgccggc tttcgtggtg gtattggcgt ggcttttcct gggagagaag
      301 ctggggaggg ccaagatagc cgcgctcgtc ctgaccttct gcgggatctc gctcgtcgcc
      361 ggcgcctacg acccggccaa cctggaggtc agcccggcgg tgctcgcgac cggcctgctc
      421 tctggtctga cttacggcct gtactcgatc ttcggccgtc ccgtggccgg tcgcctgcct
      481 ccgtccgtca tcctgagcta cgcgctgttc ttcggaggca tactgctgct ggctttcgcc
      541 gttccgacgc tcggcaccct aaccggtctc tctcccgtct cctacgcgat cctgctaatg
      601 ctcgcggtgg tccatacgac gctggccttt gccctgtaca ccttcgggtt gggtaggctc
      661 ggggccgggc gttcggccat cgtggcgacc gtggagccgg tggtagcggg cgcgctcggt
      721 gtcctgctgc tcggcgaagc ggcgaccgcg gcaaaggtcg tcggcgcgct gctcgtgctc
      781 gccggagcgg cgctggctca gttgaggacc aggaaggcgc gggtctcgta gggcattctc
      841 cttggccttt gtgctccaaa ccgggtacta tgtcgcggca cgggaaagat acggatgagg
      901 tcttctagcg cgaaatccag aaagctctgg gcttgagcag gcccgtattg ggaaaggaga
      961 ggccgtgccc atagagttca acggcagcgg tggctacacc ctgggagtcg aggaagagtt
     1021 gcacatcgtc gacgccacga ccggcgagct ggtcccgaag atcgaggaga tcatggctcg
     1081 tctacccgag gacctctgcg agttcgtctc ctatgagctg tttcagtccg tacttgagat
     1141 caagacctcc gtctgccgga acgtcgccga ggccgagaag caactgcgcg agctgcggag
     1201 ccgggtcaac tcctgggcgg ccgcctgcgg cgcgtcgctc gcggcggcgg gtacccaccc
     1261 cttctcccgc tacaaggacc agaagatcac ggaccaggaa cgctacagga aggtgatcga
     1321 gacgctccgc tgggtcgccc agcgcgaggt gatcttcggc cagcacgtcc atgtcgccgt
     1381 ccccggcccc gaagaggcca taatcgctca caaccgcctc tctgagcagg ccccgctcct
     1441 tctggcgctc tcggcgaact ctccgttctg gcaggggatg gacaccggct tcgagtccac
     1501 gcgggtgaag atcttcgaga ctttcccgcg cgcggggatg cccccggcct tcccggactg
     1561 ggagtctttc gaaggcttcg tggacatgat ggtcgagtcg ggagctatgg acgactacac
     1621 ctactgctgg tgggacgtgc gcccgcaccc gaagataggg actatagagc tacgcatcct
     1681 cgacacccag accggcctcc ggcccgccat cgccctcacc gccctgaccc agtgcctcgt
     1741 ggcccgcgcg ctggaagaga tcggccctca cgttccatat gaccgcgaac tcgcgctgga
     1801 gaacaagtgg cgcgcctccc gccacggcat ggacgccgcc ttctacgacg cgaaagaaaa
     1861 gttcaacgtc cccgcccgcg acatggctcg cgacctcgtc gaacaactga agccccactc
     1921 ccaggacctc ggctgcgaga gcgagctgct agaggtgctt gagatagtag agggagggac
     1981 tggcgcccag cagcagcgtg aggcctataa ggagagcgac gacttcctgg acgtggtcgc
     2041 gttcctcata gaaggaaccc gccccgcgct cgccggggag cagagctgat caccggcgct
     2101 ggccggagtg gaagctaaga cctttcgcga tccgtccggg catcggactc ttcgtcacga
     2161 tccagcttct tcgtctcctc ggcgtgggcc tgggtcttgt gctcctcatc cctctcttcg
     2221 agcccctggc ggaactctct catccccttc cccagccccc ttgcgagctc ggggatgcgc
     2281 ttcccgccga agaacagcag gatcaccagc acgataaaca acaggtgggt tggtgactcc
     2341 agtcctgtaa acatgcgttt ctcctctgcg gcggtggtct ctagcgtacg ccggacaccg
     2401 aaatgatact accgaaacga caacaatggg ccaccgcgat taagcccgcc tcgacccgga
     2461 atctaccgct acggttcgga gaccctactc gtcctcgaac ccgatagacc aggccctgtc
     2521 gagatcgtag ttggagtagg tgcggaaggc gaccatcgtc tcggtgcggg cgacgccggg
     2581 gacctgcgcg atcttctcgg gcactatctc cgcgagccgc tcgaagtccg ggatacggac
     2641 catcaccacg aggtcataag ggccggtgac ggagtacgcc tccgtgacgc cctctatccc
     2701 gagcatcgcc tgagccgcct cctgaacccg ctcgccctcg gtggtgatta gcacgaccgc
     2761 ggttaccaag ctaccctcct acagtccaaa ggtcaccgtc ccggtgagcg tgttgttgtc
     2821 cgtatagtcc tcgtacttga ccggtccgac ctccacggtc acgtccgcgg tctcatcata
     2881 ctcgccgggc cggaagcccg ataccttcac cgtctgcgtg ccgctgttcg gggctatgct
     2941 ctctacggtc accttctggg actggcgctc ggccttggtg ttcaagatta cctccacggg
     3001 cacggcctgc tcggcgacct cgccgccgtt cgttaccgtg acggtgaaga ccgtctccga
     3061 atcacctgtc aacaccacgt tgcccccctg atagag
//
LOCUS       DASSMB010000206         4999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_812823, whole genome shotgun sequence.
ACCESSION   DASSMB010000206 DASSMB010000000
VERSION     DASSMB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812823"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFJ72_12385"
     CDS             <1..874
                     /locus_tag="VFJ72_12385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7480304.1"
                     /translation="GGSSMDAAKGISLHAANGGSVLELDYRNTPERPGFPVIAVPTTA
                     GTGSETNGFGVIMDHASGRKFYAGHASVRPEVAVLDPLLTVGLPPAATAATGVDALTH
                     ALEALMSRNGNPYAEGLALQVIRMVATWLPVAVRDGDDVEARSRMLMAAHLAGLAFGS
                     GTGLGLCHAIAHSISARLEAAHGVALASVLPQVMAFNLPASADELALAATSLGVSDPG
                     AGLEANAAAAVEAVERLVAEVMGRLTPGSLGVTGDVLPVLVRDTIEDGVISNTPRMPS
                     PEEVESLLVMDNSG"
     gene            1101..2153
                     /locus_tag="VFJ72_12390"
     CDS             1101..2153
                     /locus_tag="VFJ72_12390"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7480305.1"
                     /translation="MNTNGSRAGLLERLAEGPVICAEGYLFELERRGYLQAGAYVPEV
                     VLEHPEEVEQLHRQFVHAGSDVVEAFTYYLHREKLRLIGREGDLEKINRQALTIARGV
                     AEETGTLFAGNICNTNVYGPGDEESIRETRKMFEEQVGWAVEAGVDFIIGETFSYAGE
                     ALIASEVIKETGLPAVITLTVHRDPETREGWTVPDACKRVEDTGADVVGLNCIRGPWT
                     MLPLLREILPGLSVPVAALPVPYRTHEGHESFQSLQDPDYDNLPTGIPFPTALDPFTC
                     NRYEISDFTREAYDLGVRYFGVCCGAGPHHIRGMAEALGRTPPASRYSEDMSKHAFLG
                     TDPTLRSQNTDFSDKM"
     gene            2382..3500
                     /locus_tag="VFJ72_12395"
     CDS             2382..3500
                     /locus_tag="VFJ72_12395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480306.1"
                     /translation="MLAAATVATFFGFQLLLSVVPLYAEEIGGGSSGAGLATAVFMLS
                     TVLTQVRMPRILARLGYRRAWMAGLLFLGLPTFLYPVTRSVIEVLAVTLARGVGFGMV
                     TVIFMALVVELAPPERRGEALGVMGVAITLPNIFSNPLGLWLVDTSGYGLVFVLGGVA
                     FLAGLACALGIRDLPSAGKNNSGSGFFDGLRRGPLLRLFVLFGMATSAGGVVVTFLPL
                     AIREAGVYSAASALLVLGVASTASRWWGGRFGDRGDPHALLAPGLISAACGMAALSQG
                     GPIMLGGALLFGAGFGLLQNSTLLLTMDRVSEDERGLGSTLWNVAFDAGTGAGALFFG
                     FLVSATGFGAAFYISAILLLAAVALAFTDRYYQRASGP"
     gene            3796..4674
                     /locus_tag="VFJ72_12400"
     CDS             3796..4674
                     /locus_tag="VFJ72_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET7480307.1"
                     /translation="MEFIQPSDWQEALEAKAAHPEAAPIHGGTDVMVELNFARSRPQT
                     ILDLTRVPEITEWGEEDGLLRVGAGVTYTRIIAELGDRLPGLAMASRTVGSPQIRNRG
                     TVGGNLGTSSPAGDGLPPLYVSDAEVEISSAEGIRRVPVAEFVVGPKRNVLRPDELIS
                     AFYISEAEGPQQYAKIGTRNAMVIAVCSFGLALHPAQQKVGTCIGSAGPTPIRSGEAE
                     AFIEGVLEEEGLWESRGEIGDAALVRFGELAAMSARPIDDVRGTAEYRRHAVGVLARR
                     TLKWAWDEYREVTDAA"
     gene            4664..>4999
                     /locus_tag="VFJ72_12405"
     CDS             4664..>4999
                     /locus_tag="VFJ72_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7480308.1"
                     /translation="MRLRLEVNGEIREVEDVWEGESLLYVLRERLELPGSKNACEQGE
                     CGSCSVYLDGTLVCSCLVLAGQAEGREVVTVEGISEGEELHPVQEAFIEAGGVQCGFC
                     TPGFIVATHD"
BASE COUNT          824 a         1547 c         1735 g          893 t
ORIGIN      
        1 tggcggttcc tcaatggacg cggcgaaggg gatctcgctg cacgccgcca acggcgggag
       61 cgtgctggag ttggactaca ggaacacgcc ggagcggccc ggtttcccgg tcattgccgt
      121 gccgaccacg gcgggcacgg gctccgagac caacgggttc ggggtcatca tggaccacgc
      181 gagcggccgg aagttctacg ccggacacgc gagcgtgcgg ccggaggtgg cggtcctcga
      241 cccgttgctt accgtcggat tgccgccagc ggcgaccgcc gcgaccggcg tagacgccct
      301 gacgcacgcc ctggaggccc tgatgtcgcg caacggaaac ccgtatgccg agggtctggc
      361 gttgcaggtc atccgcatgg tggcgacttg gttgccggtc gcggtgcgcg acggcgacga
      421 cgtcgaggcc cggtcccgga tgctgatggc ggctcacctg gcgggtctgg cgttcgggag
      481 tggaacaggg ctcgggctgt gccacgctat cgcgcactcg atcagcgccc ggctggaagc
      541 ggctcacggt gtcgccctgg cttcggtgct cccgcaggta atggccttca acctccccgc
      601 aagcgcggac gaactcgccc tcgcggcaac ctctctcggc gtctcagatc ccggcgcggg
      661 ccttgaggcg aacgccgccg cggctgtcga ggcggtggag cggctcgtgg ccgaggtcat
      721 gggccgcctc acgccgggga gcctcggcgt taccggagac gtgctacccg tcctggtccg
      781 ggacacgatt gaggacgggg tgatctccaa cacgccccgc atgccctcac cagaggaagt
      841 cgagagcttg ctggtgatgg acaactccgg gtaaacaacg cttgagacag cgatgaagct
      901 gtccgggatg gatttgccaa gatcctgaat cctgccgtat acggcgtctt tcggatgtgc
      961 taattttcgc gccaggtggt tgagagggcg acagaaagat cggagccggc ggcaggtctc
     1021 ttgttcgggc cgctctctct ggaagatatc gggcagtcag caggcgaggg gttctctctc
     1081 gtggagtcag aggaggtctt atgaatacga acggttcgcg ggcggggctt ctggagaggc
     1141 tggctgaggg tccggtaata tgcgcggagg gctacctgtt cgagctggag cgcagggggt
     1201 atttgcaggc cggggcttac gtgccggagg tcgttctgga gcatcccgag gaggtggagc
     1261 agctccaccg gcagttcgtg cacgccggct cggacgtggt cgaagcgttt acctactacc
     1321 tgcatcggga gaagctgcgg ctcatcggcc gggaaggaga cctggagaag atcaaccgcc
     1381 aggccctcac catcgccagg ggggtggctg aggaaacggg tacgctcttc gccgggaaca
     1441 tctgcaacac gaacgtctac ggccccggcg acgaggagag catccgcgag accaggaaga
     1501 tgttcgagga gcaggtcggc tgggccgtgg aggccggggt ggacttcatc atcggggaga
     1561 cgttcagcta cgcgggggag gccctgatcg cctctgaggt cataaaggag acggggctgc
     1621 ccgccgtgat tacgctgacg gttcaccggg acccggagac gcgcgaggga tggaccgtgc
     1681 cggacgcctg caagcgcgtc gaggacacgg gggccgacgt ggtcgggttg aactgcatcc
     1741 ggggtccgtg gactatgctg ccgctgttgc gcgagatctt accgggcctg agcgtccccg
     1801 tcgcggcgct gccggtcccg taccgcacgc acgaggggca cgagagcttc cagtcgctgc
     1861 aagatcccga ctacgacaac cttccgacgg ggattccgtt cccgacggcg ctcgacccgt
     1921 tcacctgcaa ccggtatgag atctcggact tcacccgtga ggcttacgac ctcggagtgc
     1981 gctacttcgg cgtctgctgc ggcgccggtc cgcaccacat ccggggcatg gccgaagccc
     2041 tcggccgcac cccaccggcc agccgctact cggaggatat gtccaagcac gccttcctcg
     2101 gcaccgaccc gaccctgcga agccagaaca ccgacttcag cgacaagatg tagacgcgcg
     2161 ggattgccgg tgtgcgggat gggccggcgg gtcctcgccc tgcgctgtag acttcggcga
     2221 gcggtggact tcacggtgtt cgagcgcacg ccgcgttcgc cgctgctcgc cccagcggca
     2281 tcgtcttctg ccgcgcatca tggctctgct tagaggcgac aaggagatcc gagataaaga
     2341 gggaccggaa gaggggtagt ctgttcactc ccgctgtagc tttgctggcg gcggctaccg
     2401 ttgccacgtt ttttggcttc cagcttctgc tctcggtcgt ccctctgtac gcggaggaga
     2461 tcgggggcgg cagttccgga gcggggctcg ccacggcggt cttcatgctc tccacggtac
     2521 tgacgcaggt gaggatgccg cgtatcctgg ctcgcctcgg gtaccggcgg gcgtggatgg
     2581 ccggcttgct cttcctcggc ctgccgacgt ttctctatcc cgtgacccgg agcgtgatcg
     2641 aagtcctcgc cgtgaccctg gcgcgaggcg tcgggttcgg gatggttacg gtgatcttta
     2701 tggccctcgt cgtggagctg gcgccaccgg aacgccgggg tgaagctctg ggggtgatgg
     2761 gcgtggcgat cacgcttccc aacatcttct ccaacccgct gggcctctgg ctcgtggaca
     2821 cctccggcta cgggctcgta ttcgtcctgg gcggagtggc tttcctggcg gggctcgcgt
     2881 gcgctctcgg catccgggat ctcccctcag caggcaaaaa caactccggc tccggcttct
     2941 tcgacgggct gcggcgggga cctctcctgc gtctcttcgt gctcttcggc atggctacct
     3001 ctgccggcgg ggtcgtcgtt accttcctgc cgctggcgat cagggaagct ggcgtctact
     3061 cggcggcgtc cgcgcttctg gtactcggtg tggccagcac cgcgagccgg tggtgggggg
     3121 gacgtttcgg cgaccggggc gaccctcacg cgctcctcgc tcccggcctc atcagcgccg
     3181 cctgcggaat ggcggcactc tcgcagggcg gccctatcat gctcggcggc gcgctgctct
     3241 tcggcgcggg gttcggactg ctccagaact cgacgttgct actcacaatg gatcgcgtct
     3301 cagaagacga acgcggtctg ggcagcaccc tctggaacgt ggctttcgac gcgggcaccg
     3361 gcgccggcgc tctcttcttc ggcttcctcg tctcggccac cggcttcggc gcagcatttt
     3421 acatatcggc gattttgctc ctcgccgccg ttgcgctcgc cttcacggac cgttactatc
     3481 aacgtgcgag cggcccatag aagatcccga agcaaccccg gtaagagcct acagatttgg
     3541 cctcggacct ctccagaaaa attcccgccc cgaccgaata aacccttgac atggagatca
     3601 cagcctgggt atactacccg gccagtagaa aataatgtcc gtgatacgga ataaagggag
     3661 aaggaaaagg cgcgtttggg agcgatcacc tagctgcaag gtccctggtt gaggatccgg
     3721 aaaaggcgcg cgacaaacga tcacctggct tgatctccca tatagcgctc taatgagacg
     3781 cgaagggagg caaagttgga gtttattcaa ccttctgact ggcaggaggc tctggaggcg
     3841 aaggccgctc accccgaggc ggccccgatc cacggcggca ccgacgtcat ggtggagctt
     3901 aacttcgccc gcagccgtcc gcaaaccatc ctggacctga cgcgagtccc cgagataacc
     3961 gagtggggag aagaggacgg cctcctacgg gtaggcgccg gagttaccta tactcgcatc
     4021 atcgccgagc tgggcgacag gctccccggt ctcgcgatgg cgtcccggac ggtggggtcg
     4081 ccccagatcc ggaaccgcgg caccgtcggc ggcaacctgg ggacctcttc ccccgccggc
     4141 gacggcctgc cgccgctgta tgtctccgac gccgaggtgg agatctcatc tgccgaaggt
     4201 atccggcggg taccggtcgc ggagttcgtc gtcggcccca agaggaacgt gctccggccg
     4261 gacgagttga tctccgcttt ctacatttcc gaggccgaag gtccgcagca gtacgccaag
     4321 atcggcactc gcaacgctat ggtcatcgcg gtctgttcct tcgggctggc gctgcatccg
     4381 gcccagcaga aggtcgggac gtgcataggc tccgccggtc ctaccccgat ccgtagcggc
     4441 gaagccgaag catttatcga aggcgtgctg gaggaagaag ggctatggga gtccagagga
     4501 gagatcggag acgcggccct ggttcgtttc ggagagttgg cggcgatgtc cgcgaggccc
     4561 atagacgacg tgcgggggac cgcggagtac cggcgccacg cggtcggcgt gctggcgcgg
     4621 cggacgctca agtgggcctg ggacgagtac agggaggtga ccgatgcggc ttaggctgga
     4681 agtcaacgga gagatcaggg aagtagagga tgtctgggag ggcgagagcc tcctctacgt
     4741 gctgcgtgaa cggctggaac tgcccggctc caagaacgcc tgcgagcagg gcgagtgcgg
     4801 ctcctgctcc gtgtacctgg acggcacgct cgtctgctcg tgcctggtgc tcgcggggca
     4861 ggccgaaggg cgagaagtcg tgacggtcga gggcatctct gagggagaag aattgcatcc
     4921 cgttcaagag gcgttcatcg aggcgggggg tgtgcagtgc ggcttctgca cgccggggtt
     4981 catcgtggcg acgcacgac
//
LOCUS       DASSMB010000207         7752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_171443, whole genome shotgun sequence.
ACCESSION   DASSMB010000207 DASSMB010000000
VERSION     DASSMB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7752
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171443"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..383)
                     /gene="pqqA"
                     /locus_tag="VFJ72_12410"
     CDS             complement(288..383)
                     /gene="pqqA"
                     /locus_tag="VFJ72_12410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HET7480309.1"
                     /translation="MELQKWETPEFEEIGTSAEVTAYAGIWPDSE"
     gene            complement(574..2265)
                     /locus_tag="VFJ72_12415"
     CDS             complement(574..2265)
                     /locus_tag="VFJ72_12415"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HET7480310.1"
                     /translation="MTQEYIDAPPPVALGNLSATTHNAPPVTKDLTYERILDARSEPQ
                     NWITYYGAYDGKRFSPLDQINAENVKRLSPAFVFQSGSQGLHAGPSTYAFEAAPLVVD
                     GVMFVSGWDGFLWCLDATNGQTLWQYKHAIPFDVSLCCGNVNRGVAVAEGKVFLATLN
                     AHVVALNAENGEKIWDTTHGDVRAGESCTVAPLVVKDMVIVGSSGGEFGVRGHLDAYN
                     IDTGERVWRCYMVPKPGEPGSETWPEDGEAWQRGGGNCWVTGTYDPELNLLYWGTGNP
                     APDFDGEVREGDNLFTDSVVAVNPDDGTIQWHYQYTPHDLWDYDSNMENILFEQDGRK
                     LLGHFDKNGYFFVLDRTNGELVRVTPFVDRINWGEIDSDGKVTPSIYPEKEGEEVHFF
                     PGPAGAKEWTHACFNPETEMFYCPVQDTGSTIARRRREFKEGMPYWGAHVIVDSEDMK
                     GYVSAFSASTSEEVWRWETDLPMCASVLTTAGNLVFAGEPTGYLNALDARTGEHLWRF
                     QCGSGHHSSPTTYSVDGRQYIAVPVGWGAWVEGFLPGLMGGPHGSAIFVFALPEE"
     gene            complement(2293..2727)
                     /locus_tag="VFJ72_12420"
     CDS             complement(2293..2727)
                     /locus_tag="VFJ72_12420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:1901661 - quinone metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_3727 family PQQ-associated protein"
                     /protein_id="HET7480311.1"
                     /translation="MVTELGLDKQNKGTIGRLLNHGGIGQVEEDADGRMKIQVRIPED
                     ELTYSPSITVMPHAGELEIEFLNDDTNTHCAQVPSNGDYQWIWLPNGSRGKVTVTLDG
                     PGYYWYGSNVGNDEGRGLTGAIVVMGDVPEEARLDRPAQPRP"
     gene            complement(3022..4965)
                     /locus_tag="VFJ72_12425"
     CDS             complement(3022..4965)
                     /locus_tag="VFJ72_12425"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7480312.1"
                     /translation="MDTARDTSKVNTARDEPRVSATPTVDEYSRDLTQMAKEGKLDPL
                     IGRVEEVETIIEILCRRTKNTPVLVGDPGVGKTAIIEGLAQWIVNGEAPEALADKRVL
                     ALDLSGMVAGTQYRGEFEERFKKVIEEIRENPDDQIIFLDEMHTVVGAGVVEGSMDAS
                     NMLKPALAYGEMCLVGATTVEEYRKNIEKDAALERRLQPILVSEPSVEESIEILRGLK
                     DRYEAHHRVEITEEAIASAAELSDRYIAGRFLPDKAIDLMDLASARVRLRAKTKPADT
                     KALEEKDLALAVEDFGKPRELDIKVAEEPDGDRRSVAEVGADDIAEVVSRATGIPVSQ
                     LTEEDRERLLRLESQLHERIVGQEEAVEAVAEAIRRARAGLSDPNRPIGSFLFLGPTG
                     VGKTELARTLAHALFGDEATMVRIDMSEFQERHAVSRLVGAPPGYVGHEDAGQLTEAV
                     RRRPYSVMLLDEIEKAHPDVFNILLQILDDGRLTDAQGRTVDFKNAVVIMTSNFGAER
                     IKAHAHKNESFEDLKEDMSRLLRSALRPEFVNRIDEVILFRSLGRDQIARIARLLLDH
                     TSRRLKARGIGIDLTDAAVALLAEEGFDPEFGARPLRRAIQRRVDNVLSRMVLDCSLE
                     PGDKVTIGAGEDGLCFEVIYGAR"
     gene            complement(5155..5340)
                     /locus_tag="VFJ72_12430"
     CDS             complement(5155..5340)
                     /locus_tag="VFJ72_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480313.1"
                     /translation="MDDLNPIAVKGAQEIAKAQRRKYGPRIEKLVQMGTTRELAEYLV
                     GLEIRVAQLEQRPFTSR"
     gene            complement(5652..5921)
                     /locus_tag="VFJ72_12435"
     CDS             complement(5652..5921)
                     /locus_tag="VFJ72_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET7480314.1"
                     /translation="MAKTHKVTFRRRGVTIECAEDEYILEKAEEAGLILPYHCRGGTC
                     TTCIVKCLEGEMDQELAFALDDLDLEQGWRLICIGSPLSDVVLDR"
     gene            6342..7388
                     /locus_tag="VFJ72_12440"
     CDS             6342..7388
                     /locus_tag="VFJ72_12440"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7480315.1"
                     /translation="MGRNVFPVVFDGHNDVLLRLGGPDGGGPDAFFERGDQGHLDLPR
                     AREGGFGGGFFAVWVPSPREPSPEPTPRGLPPALDAAYALRRTIADTAMLFRIEDRSD
                     GRFRIVRTAAELRRCLDEEVMAAVLHMEGADAIDTDLDALHVLYRAGLRSLGLVWGRP
                     NAFAEGVAVHFRRSPDTGPGLTEAGRELVRACNRLRIMIDVSHLNERGFWDVAALTDA
                     PLVATHSNAYALCASTRNLTDPQLDAIAASDGMVGLNFAVNFLREDAARNASTPLEVM
                     VRQIDYLVERVGIDGVGFGSDFDGVLISREIGDVSGLPGLMSALRDRGYDDQALRKLA
                     HENWVRVLRKTWGE"
     gene            7467..>7752
                     /locus_tag="VFJ72_12445"
     CDS             7467..>7752
                     /locus_tag="VFJ72_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480316.1"
                     /translation="MPLLFRLLSNVPPLRRRVFWLLSQLWTNYRESPAIMGPDGQVAR
                     AGDRAPYGFFEAGPEAGKSIFETLGDLDHHLLLFEGLEPDPRLEAAREEIE"
BASE COUNT         1283 a         2553 c         2335 g         1581 t
ORIGIN      
        1 atgtcgggtg aagcgttgag caggaaccat cgccggtagt cggcgctcac cgcgaccgac
       61 gactgggtgc gcggacggca gggtcgcgag ccgtcgcgca cggcccggca ctgagggcag
      121 ttgcagttcc actgcggaaa ccctcctccc gccgccgctc cgagcacccg cacccacaca
      181 cccgtcctcc atccatttac ctcgatcatt ctccccggcc gcaatcgtcc tgcggcaaga
      241 tggggacagt gcgggcgccg gaccatccgg cccggcgccc tgtacgttta ctctgaatcc
      301 ggccagatac cggcgtacgc agtcacctca gcgctggtcc cgatctcctc gaactcgggg
      361 gtctcccact tttggagttc catggctatt acctcctttc gtatggactc tgtagtgcag
      421 gctcttcgtg tcccgaccct gcacgctcac ctccatcgtc cccggcagat caggcaacca
      481 tggctcgggg ccactccgca ccgaaccgga gacgttcctc cttcgtaccg ggtggcgcac
      541 cttctacgcc acccggtacc agacatctgt gctttattcc tccggcagcg cgaaaacgaa
      601 gatagcggaa ccgtggggcc cccccatcag tccgggcagg aacccttcga cccaagcgcc
      661 ccaaccgacc ggcacggcta tgtactgcct tccgtcaacg ctgtaggtcg tcgggcttga
      721 gtggtggccg cttccgcact ggaacctcca caaatgttcc ccggtgcgcg catctagtgc
      781 gttcaggtaa cctgtgggtt cgcccgcgaa cacgaggttg ccggccgtgg tgagcaccga
      841 cgcgcacatc ggaaggtcgg tttcccacct ccagacctcc tcgctcgtgg acgcgctgaa
      901 ggcgcttacg taccccttca tgtcctcgga gtccacgatg acgtgcgctc cccagtaagg
      961 catgccctcc ttgaactccc tgcgccgccg cgcgatcgtc gaaccggtat cctgcaccgg
     1021 gcagtagaac atctccgtct ctgggttgaa gcacgcgtgc gtccattcct tggccccggc
     1081 cggccccggg aaaaagtgaa cctcttctcc ctctttctcg gggtaaatgc tcggcgtcac
     1141 tttgccgtcg gagtcgatct cgccccagtt gatgcggtcc acgaagggcg ttacccggac
     1201 cagctcgccg ttcgtgcggt cgaggacgaa gaagtacccg tttttgtcga agtgcccgag
     1261 caacttgcgc ccgtcctgct cgaagaggat attctccatg ttgctgtcgt agtcccacag
     1321 gtcgtgcggg gtgtactggt agtgccactg tatcgtgccg tcgtcggggt tcacggcgac
     1381 gacgctgtcg gtgaagaggt tgtcgccctc gcggacttcc ccgtcgaagt cgggcgccgg
     1441 gttgccggtc ccccagtaca ggaggttgag ctccgggtcg taggtgcccg tgacccagca
     1501 gtttccgcca ccccgctgcc aggcctcacc gtcttcaggc caggtctccg atcccggctc
     1561 gccgggcttc ggcaccatgt agcagcgcca gacgcgctct ccggtgtcga tgttgtaagc
     1621 gtcgaggtgg ccgcgcaccc cgaactcccc gccggagcta cccacgatga ccatgtcctt
     1681 cacgacgagg ggagcgaccg tgcagctctc accggcccgc acgtcaccgt gcgtggtgtc
     1741 ccagatcttc tcgccgtttt cggcgttgag cgcgaccacg tgggcgttta gggtggcgag
     1801 gaagaccttc ccctccgcca cggcgacccc gcggttcacg ttgccgcagc acagcgaaac
     1861 atcgaacggg atcgcgtgct tgtattgcca cagcgtctgc ccattcgtgg cgtcgaggca
     1921 ccagagaaag ccatcccagc ccgagacgaa catgacgccg tccacgacga gcggggcggc
     1981 ttcgaaggcg taagtcgaag gcccggcatg aaggccctgc gagccggact ggaacacaaa
     2041 cgcgggagag aggcgcttga cgttctccgc gttgatctgg tccagcggac tgaaccgctt
     2101 cccatcgtat gcgccgtagt aagtgatcca gttttgcggc tccgagcggg catcgaggat
     2161 tcgctcgtac gtgaggtcct tcgtcaccgg cggcgcatta tgggtcgtgg cgctcaagtt
     2221 cccgagggct acgggaggcg gtgcgtctat gtattcttgc gtcaacggat tttcctttcc
     2281 cctatggcct tgctacggtc gcggctgggc tggacggtcg aggcgagcct cttccggaac
     2341 gtctcccatc acgacgatcg cccccgtcag gccccggcct tcatcgttgc ctacattgga
     2401 gccataccag taatagcccg ggccgtcgag ggtgacggtg accttgccgc gtgaaccgtt
     2461 cggcaaccat atccactggt agtcaccgtt gcttggcacc tgcgcgcagt gagtgttcgt
     2521 gtcatcgttt aagaactcga tctcgagctc gcccgcatgg ggcatgacgg tgatcgaagg
     2581 cgaatacgtc agctcgtcct cggggatacg gacctggatc ttcatgcgtc cgtcggcgtc
     2641 ctcctcaact tgcccgatgc ccccgtggtt gagcagccga cctatcgtcc ccttgttttg
     2701 cttgtccagc ccaagctcgg tcaccacctg ggatagctgt ttctccctag cccttgccac
     2761 tagcttgcct ccttcatgtg tttccccaac acttcgcagg tgaatacagg atcgcaaaaa
     2821 caggggtcaa cccctcgccc aagtgtccgc cgttggctcg gcctccttct agtaacgtcc
     2881 cttccatgac acaaggtagc atacaaacat atcggaggtc aactcatgtt ccaattggca
     2941 ttcaatcgct gttacacggg acagaaggcc gcacctgttt ttcaggggcc ttcagggtcg
     3001 agttcctaat tcacgccgtt ctcaacgcgc tccgtagatt acctcgaagc acagtccgtc
     3061 ttccccggcg ccgatagtca ccttgtcgcc tggttcgagc gaacaatcga ggaccatgcg
     3121 cgagagtacg ttgtccaccc tccgctggat ggcgcgccgc aggggacggg cgccgaactc
     3181 ggggtcgaag ccctcttcgg ctaaaagcgc cacggcggcg tcggtgagat ctatcccaat
     3241 cccccgggct ttcaggcgac gggatgtatg gtcgaggagg aggcgcgcta tccgcgctat
     3301 ctgatccctg cccagggagc ggaacaggat cacctcgtct atccggttga cgaactcggg
     3361 gcgcagggcg ctcctgagaa gccggctcat gtcctccttg agatcctcga acgactcgtt
     3421 tttgtgggcg tgcgccttga tgcgctccgc cccgaagttg ctcgtcatga tgaccaccgc
     3481 gttcttgaag tccacggttc tgccctgggc gtccgtgagc cgtccgtcgt ccaggatctg
     3541 gagcaggatg ttgaacacgt ccgggtgcgc tttctcgatc tcgtcgagca gcatgacact
     3601 gtacggacgg cgcctcacgg cctcggtcaa ctgcccggca tcctcgtgtc cgacgtagcc
     3661 cggaggcgcg ccgacgagcc ttgagaccgc gtgccgctcc tgaaactcgg acatgtctat
     3721 cctcaccatc gtcgcctcat ccccgaagag ggcgtgggcg agcgtgcgtg cgagctcggt
     3781 cttgccgacg ccggtcgggc cgaggaacag gaagctgccg atgggcctgt tcggatccga
     3841 aagtcccgcg cgggcgcgtc ttatggcctc ggccaccgcc tcgacggcct cttcctggcc
     3901 gacgatgcgc tcgtggagct ggctctccag gcgcagcagc cgctcgcgat cctcctcggt
     3961 gagctggctc accgggatgc ccgtggcccg agagacgacc tcggcgatgt catcggcccc
     4021 cacctccgcc accgagcggc gatctccgtc gggttcctcg gccactttga tgtcgagttc
     4081 ccgcggcttc ccgaagtcct cgacggcgag ggcgagatcc ttctcttcca gggctttcgt
     4141 gtcggcgggt ttggtcttcg ctcgcaggcg caccctcgcc gacgcgaggt ccatcaggtc
     4201 aatggccttg tccgggagga agcggccggc tatgtagcgg tcggaaagtt cggcggcgga
     4261 ggcgatcgcc tcttcagtga tctcgacgcg gtggtgcgct tcgtagcggt ccttgaggcc
     4321 gcgcaggatc tcgatagact cttccacgct cggctcgctg acgaggatcg gctgcaagcg
     4381 gcgttccagc gcggcgtcct tctctatgtt cttgcggtat tcctccaccg tggttgcgcc
     4441 cacgagacac atctcgccgt atgctagagc gggcttgagc atgttggagg cgtccatcga
     4501 accctccacc acgccggctc caaccacagt atgcatctca tcgaggaaga tgatctggtc
     4561 atccgggttc tcccgaatct cctctatgac cttcttgaag cgctcctcga actccccacg
     4621 atactgcgta cccgcaacca tccccgagag atccagcgcc aggacacgct tatcggcgag
     4681 agcctcggga gcctcgccgt tgacgatcca ttgtgcgagc ccctcgatga tggccgtctt
     4741 gccgacaccg ggatctccta cgagcacggg ggtgttcttc gttctccggc agaggatctc
     4801 gatgatggtc tcgacttctt cgacccgccc gatgaggggg tcgagcttcc cttctttcgc
     4861 catctgcgtc aggtcgcggc tgtactcgtc caccgtcggc gtcgcgctga cccgaggttc
     4921 gtccctggcg gtattcacct tggatgtgtc cctggcagtg tccacctcgt catcccccaa
     4981 ttcttcgctg acgggttgga gtggcttgat gatcgatgca tttcatcttc aacatcctac
     5041 taaatgcgca cgattgtact tcgttggagc ttcccggcgt caggcgaacc cggcgagcgt
     5101 agtctatcgc atcggcgatg gctgagaatc ccggccacag cgtcctgcgg gccgttaccg
     5161 gctcgtgaac ggcctctgct ctagttgggc gacccttatt tcgaggccga cgaggtactc
     5221 ggcgagttcg cgggttgtgc ccatctggac cagtttttct atacgtggcc catacttccg
     5281 tcgttgagcc ttcgcgatct cttgcgcgcc tttgacggct atgggattaa ggtcgtccac
     5341 gtcaatttcc tttcctgatc gcctttccaa atactatccc cttttacacg aaggcatcta
     5401 gcacgtcctg cgttcttgct ggtccgcgcc gagcgggatg gcgatcaagg gttggtaagg
     5461 cactaaagcg cagggggcgg aatgcctttc cgtcccggcc cgcaagcgat aagcgaaacg
     5521 ggtcctatcc ggttggcgcg gccgagagtg attcggtgac gttcgcgccg acgatgcgcc
     5581 cgcgagccaa gctggttcaa gcttctagaa ggttccaggg tgtccctggg gacagctaca
     5641 cactccagct actatctatc cagcaccaca tccgaaagcg ggctgccgat gcagatgagg
     5701 cgccagccct gctccagatc aaggtcgtcc agcgcgaagg cgagctcctg gtccatctcg
     5761 ccctcgaggc actttacgat acaggtggtg caggtgccgc cgcggcagtg gtaggggagt
     5821 atgagcccag cttcttcggc tttttcgagg atgtactcgt cctcggcgca ctctatggtg
     5881 acgcccctgc gccgaaaggt taccttgtgg gtcttcgcca tgtctctcct attgaccgtt
     5941 gtgtactcct gatcctttta gcacatcgga ggggtgatcg atccggatag cgcccctggc
     6001 cccggcgagc tgtccccgcg attctccggc gaattaaccg acagcattac aatggactcg
     6061 aaggcgatga ccaccatgcg cccgcttggg agcgcttcga agcacacgtt cgcggaagcc
     6121 ccgaaccaga tgccgcaacc ggcgagagag gaacgcgcca gtgaagatgc accccctccg
     6181 gcgcgttgtg ccctacgttc caggtccttc gggatcttcg cggtcgtgac gctacgctcg
     6241 gctttgatgg ctgactcccc gcgaaagcta caataccagg ccatcagctt catggctcta
     6301 caatcgcgcg agattgtgtc atcaaagaga aggatgctcg tatgggtcgt aatgtatttc
     6361 cggtcgtgtt cgatggacac aacgatgtac ttctaaggct cggtgggccg gacggaggcg
     6421 gacccgacgc attcttcgag cgcggtgacc agggacacct ggacctgcct cgggcgcggg
     6481 aggggggttt cgggggcggc ttctttgccg tctgggttcc ctcgcctaga gagccgtcgc
     6541 ccgaaccaac tccgagaggg cttccacccg cactcgatgc cgcgtacgcg ctgcgccgca
     6601 cgatagccga tacggccatg ttattcagga tcgaggaccg ctcggacggg aggttccgca
     6661 tcgtccgcac cgccgccgaa ctgcggcggt gtctggacga agaggtaatg gcggcggtgc
     6721 tccacatgga gggcgccgac gcgatcgata ccgacctcga cgcgctgcac gtgctctacc
     6781 gggcggggct gcgctcgttg ggcctcgtct ggggccgtcc gaacgctttt gccgaggggg
     6841 tcgccgtaca ctttcggcgc tcgccggata cggggccggg gctaaccgaa gccggtaggg
     6901 agttggtccg ggcctgcaac agactccgca tcatgatcga tgtgtcccac ctgaacgagc
     6961 gaggcttctg ggacgtggcg gctctcacgg acgccccgct cgttgcgacc cactccaacg
     7021 cttacgccct ctgcgcctcc acccgaaacc tcacggaccc gcagctcgac gccatagccg
     7081 cctccgacgg aatggtcggc ctcaacttcg cggtcaactt cctccgagag gatgcggcgc
     7141 ggaacgcaag cacccccctt gaagtgatgg tccggcagat cgactatctc gtagagcgag
     7201 tcgggatcga cggggtcggc ttcggctccg acttcgatgg cgttttgatc tcgcgtgaga
     7261 taggtgatgt gtcgggcctg ccggggctga tgtcagcgct cagggaccgg ggctacgacg
     7321 accaggcgct ccgaaagctc gctcacgaga attgggtccg tgtcctgcgc aagacctggg
     7381 gcgagtagac cgactccgcc tctcaactgt ctcgcgccga gatagcctct ccacacccgg
     7441 ccgcgcgaaa gctcaagccc gtcttcgtgc cgctgctctt caggctcctg tcgaatgtgc
     7501 cgcccctcag gagacgggtc ttctggctcc tctcccagct ctggaccaac taccgcgaga
     7561 gcccggccat tatggggccc gacgggcagg ttgcgagagc cggcgaccgt gccccttacg
     7621 gcttcttcga ggccggcccg gaggccggca agagcatctt cgaaacgctc ggagacctgg
     7681 accaccatct gctcctcttc gaggggcttg aaccggatcc caggctggag gcggcgcggg
     7741 aggagatcga gg
//
LOCUS       DASSMB010000208        10052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_564041, whole genome shotgun sequence.
ACCESSION   DASSMB010000208 DASSMB010000000
VERSION     DASSMB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564041"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VFJ72_12450"
     CDS             complement(<1..621)
                     /locus_tag="VFJ72_12450"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7480317.1"
                     /translation="MSARPNIVVIVSDTFRRDHLGAYGNPVIRTPYLDEFASSAVTFD
                     RHVISSFPTMPARADILTGTFSFTYMGWEPLPRHVPTLPGLLSEAGYLTMGVVDTPFF
                     VRGGFGYDRGFDDFIWVRGQGDDTRPHERSDYRATWRSESDRLVARTVTEAESWLERQ
                     HDEQFFLYVDTWDPHEPWDAPDYYTSRYHAGYDGRQIYPSYGRWEQA"
     gene            complement(648..860)
                     /locus_tag="VFJ72_12455"
     CDS             complement(648..860)
                     /locus_tag="VFJ72_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480318.1"
                     /translation="MAIFLCWLRLSLRFMRYLSGDPSALERFLQKRRGHLRWWSYGAF
                     GSKGRQEGGQYLAGRRAPPLLHLFSA"
     gene            1471..2229
                     /locus_tag="VFJ72_12460"
     CDS             1471..2229
                     /locus_tag="VFJ72_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480319.1"
                     /translation="MSGIRFDMVEEAMRVAGEAERAGQDLRLLGGVAVKVRAKNGLEP
                     AFRREYADLDWITPKGTSAGAQKVFESLGYTPQVRFNALYGQERLLFFDEEHDRQVDV
                     LVGSFRMCHEIRFGKRLAIEPVTIPLAELLLTKLQIIQLNEKDVRDALAILHDHPVEE
                     GDGDAVNGGRIAELCASDWGLWRTITANLEALGGHAERYELPGDGKERIAERVGSLLG
                     RIEEEPKSFGWKMRAKIGDRKRWYDLPEEVDGGP"
     gene            2230..3204
                     /locus_tag="VFJ72_12465"
     CDS             2230..3204
                     /locus_tag="VFJ72_12465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480320.1"
                     /translation="MISRFFNREPETRLFFATDIHGSETCWRKFLNSGKHYEAKVMVL
                     GGDMTGKALVPIVEESAGRWYATLLENRRDFTTEDEVKEFEDSVRRRGYYPFRTDPEE
                     MAELESNEQLRHDLFHKEMLGGVERWMRMADEKLEGTGIECFVSPGNDDQFEVDEIIA
                     NAKRVKLAEGRVMEFGDFQMVSTGWANRTPWDTYREEDEPDLAARLKKMTSQVTAAPE
                     KTIYNFHCPPYHSGLDEAPEIDADMRPKHGGRSIVPVGSTAVREAIEEGQPALALHGH
                     IHEARGNTRIGRTLCINPGSSYEQGQLLGAVVDLDGGKKVKRFVLTSG"
     gene            3215..4831
                     /locus_tag="VFJ72_12470"
     CDS             3215..4831
                     /locus_tag="VFJ72_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7480321.1"
                     /translation="MASNSTAGSSTSDLFQRKATGLLKGWSVWDGFIYAAFAINLITL
                     GLYIFSFAPFIPDGSLIFARVLSGLYLVFQAVTYASLIAVMPRAGGDYVWMSRVLGGG
                     IGFVLAVTGWWFILWHWVPIYANILNIQVLVPLATIVGLKGVADFFVSPGGVFTASMI
                     VAVIASVLIALGMRTYARVQKFCFYGGVIGLAIMLVLLLFHSKADFISAFNANAKELY
                     GASGDAYQQTLKAGAVKGASPGGFVFGPTLFLIPMIVFFNLWSNWGATLYGEVRGASD
                     FRKNIYAMGGALLATSFVAVIMLLLFAKTFGWDFYYAASNAYWSGAKDAPVSAWPYPG
                     LFAAFFFDSPILQFILVALLSLWFFGWVGSVFLSSTRVVFATAFDRILPEWVSKTSRN
                     GVPYAALALMLIPSIPISYLYAFGENFATYTLDATLVIAMTFLGSAIAAAVLPWRKPE
                     IYNASPIARYNVLGVPLITASAVLFSVFLIFCLYQWLSDPVYGINNGQSLIYMVVLYV
                     VAIVMYVAFRLVRRAQGMDLKMVYDEIPEE"
     gene            5166..5486
                     /locus_tag="VFJ72_12475"
     CDS             5166..5486
                     /locus_tag="VFJ72_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480322.1"
                     /translation="MSNEPRSYRESIASVLRDLRPNAEVKIVEQEELERWILRFAPDL
                     AICNQATYAVRSRVPVWVELYPEGRAVSTISIKGRRERSDGIDLSRLLEIVDFAEVVA
                     DARE"
     gene            5715..7670
                     /locus_tag="VFJ72_12480"
     CDS             5715..7670
                     /locus_tag="VFJ72_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7480323.1"
                     /translation="MSESVRAQSRLEKVASQEDSFHGGRFAAAFEQVAVGMAKLGLDG
                     GFLSVNRKFCEIVGYSRNELLDSVAYMEIVCREDRDDHRQDFRTLLADGEDTCSVERR
                     CVRSGGEVFWANITASLVRNDEERPAFFVIVVEDISERKRAEEALKESEELYRAVIEQ
                     AAEGIYLFDPEAMEILESNRALQEMLGYSAEELLGIPVYRLIVDERKSINANALRVIL
                     NDSYSFGERKYRRKDGSLVDVEVSGTRIFYGGKEAIYAVVRDVTERKEVEENLRQSLS
                     VLLALREAGQILGSTLELEEIGSRLLEIMKNVSNLTTTVISVQEDNGQLRIWRSVGLE
                     SLWSGARFFPAAEEARRVAMEEEEPRLFRLKPPGASRSSVMGLCLPLRMRDHVFGVLE
                     AYGPDSLAESDTMEVLRSITNQAASALENARLYGELAERERRLQELIGKLIVAQEEER
                     HRVAYEVHDGLAQVAVAAHQHLQAFAERHPPEMDHARADLQVVLKLVRRTVSDARRII
                     ANLRPTVLDDLGLAAALALEVEQMADEGYEVEYHEALGGERIPGPIEIAFFRVAQESL
                     TNMRKHARTRWARIDLERRDHQARLVVRDAGRGFDPRSEFASSGPGERVGLAGMRERI
                     GSLGGRLEIDARPGVGTSIIATVPLEA"
     gene            7801..8511
                     /locus_tag="VFJ72_12485"
     CDS             7801..8511
                     /locus_tag="VFJ72_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480324.1"
                     /translation="MRNTKLPTSKLSTAARLIIVDDHDMAREGLRDMLADVPDIEVIG
                     EASNGKEALVLCSMLRPDLALMDVRMPEMDGLLATSKIKQSHPEISILMVTMHENPEY
                     LFEALKAGAAGYVLKDASRQEVIDAVRQVRLGESPLDPDLASRLLRRIAAESTPGRRL
                     SGGENRHRFDKLTRRELEVLGLLKEGMTNRQIAGVLFVSPGTAKNHVEHIITKLGVSD
                     RTQAVVRALEIGIIDFNS"
     gene            8614..9024
                     /locus_tag="VFJ72_12490"
     CDS             8614..9024
                     /locus_tag="VFJ72_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480325.1"
                     /translation="MRKIFAQIQEGATNQGKGTLAVCGDPVAGRALVLLLRGADYDVK
                     FLPLSVLEEPGMLEGVQLLILGLTPGLNEEGRGALLKFLRSSPFAASMPTLELVVPEG
                     AQNAEPRDSRLVVPWPCSTEQLKERIETALRTRL"
     gene            9310..>10052
                     /locus_tag="VFJ72_12495"
     CDS             9310..>10052
                     /locus_tag="VFJ72_12495"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480326.1"
                     /translation="MSSIMEAGIRQSAPTPGSYSVAARSQLKRAVGQAVVVPALILSD
                     VVLATAVWEIVFAVQQVFGHAQLSIVSVVGFFPYVAAWVGLRALSGLYPGYGMGQIEE
                     LRRQTLALLTTLAITAVFALASQAEDVLSRLMLFGCSLGLLLVAPLVRYGVKHVLMRT
                     GTWGKPVVVLGAHEAGARMLRALRREWQLGLKPVGVFDNHRVPAGGEIEGIPYGGTLT
                     DALSAAGQLGIETAIFAMPYTRRESLAKLV"
BASE COUNT         1939 a         2939 c         3311 g         1863 t
ORIGIN      
        1 ggcctgctcc catctgccgt agctcgggta gatctgccgt ccgtcataac ctgcgtggta
       61 gcgcgaggtg tagtaatccg gcgcgtccca gggctcgtgc gggtcccagg tatccacgta
      121 gaggaagaac tgctcgtcgt gttgccgctc cagccagctt tcggcctccg tgacggtgcg
      181 cgccacgagc ctgtctgact ccgacctcca ggtagcgcgg tagtcggagc gctcgtgcgg
      241 ccgggtgtcg tcgccctggc cccgcaccca gataaagtcg tcgaagcccc ggtcgtagcc
      301 gaaacctccc cggacgaaga acggcgtatc cacgacgccc atcgtgaggt agccggcttc
      361 ggagaggagt cctgggagtg tgggcacgtg acgggggaga ggctcccagc ccatgtaggt
      421 gaaggagaag gtgccggtca ggatgtccgc ccgcgccggc atggtgggga aagaggagat
      481 cacgtgccgg tcgaaggtga ccgcggaact cgcaaactca tccaggtacg gcgtgcggat
      541 cacggggttc ccgtaagccc ccaggtgatc ccggcgaaac gtgtccgaca ctatgaccac
      601 gatgttcgga cgtgctgaca tactggtcct cctgcattct gttggatcta ggcgctaaag
      661 agatgcagga gcggcggggc gcggcggccc gcgagatatt gaccaccctc ttgacggccc
      721 ttgctcccga aagccccgta ggaccaccag cgcaggtgcc cccggcgctt ctgcaggaaa
      781 cgctccaggg cggatggatc gccggacaga taccgcatga aacgcaggga caggcgaagc
      841 cagcacaaga agatcgccaa ccgtctaccg ccgacgacgg tagaggcccg gaaccaacca
      901 tgagaaccag cccgaaaaga catcggcgaa ccacacgcta cactactaac atgttgcgta
      961 tgataaaaca cctttcccat agaacaacaa atatcatacg ttatatgact cgtcaatagt
     1021 tcgggtaggt gatttttcgg ggagtatggc tcgacctctc actgtccgga gaattcgtga
     1081 gatgtctacg aaatccggat aaacacaggc tatatcccgc accccacggc gggtctgatg
     1141 taataaaata tatatgatac atgtgccatt gggagagaag aagtttgcat tacgcaacgc
     1201 aacccggaaa ggcccgtgag tagggctcgt caatttcgcc tggggcttcg gggagtggga
     1261 ccatgacgct gttctacggg ggttatcggg ttggcggcgc ggcagttatc gaaaatccct
     1321 gtagtcgggc attgtgctat cgttcctgtg ttctgtcagg gcattgcaag cgagacaacg
     1381 tggggaaaag acgatgaaaa catcggcgga ctccggcgag tatgcgtatg ggacggagcg
     1441 attcaccaag tccgcgtcgg aggcgaaggt atgagcggca tccggttcga catggtggaa
     1501 gaggcgatgc gtgtcgccgg ggaggctgag agggcggggc aggatctccg tctgctcggc
     1561 ggggtggcgg tcaaggtgcg ggccaagaac ggtctggagc cggcgttcag gcgcgagtac
     1621 gcggacctcg actggatcac gccgaaaggc acgtccgccg gggcgcagaa agtcttcgag
     1681 tctcttggat atacgccgca ggtccgcttc aatgctctct acggccagga gcggttgctg
     1741 ttcttcgacg aggagcacga ccggcaggtg gatgtgctcg tcgggtcctt caggatgtgt
     1801 cacgagatcc ggttcggaaa gaggctcgcc atcgagcccg tgacgatccc gctggcggag
     1861 ctgctgctca ccaagctcca gatcatccag ctcaacgaaa aagacgtgcg ggacgccctg
     1921 gcgatactgc acgatcatcc cgtcgaggag ggcgacggcg atgccgtgaa cggcggccgc
     1981 atcgcggagt tgtgcgcgtc tgactgggga ctctggcgca ccatcaccgc caacctcgaa
     2041 gcgctgggcg ggcatgcgga gcgctacgag ttgcccggcg atgggaagga acgcatcgcg
     2101 gagcgcgtcg gtagtctgct cggacgaata gaggaggagc caaagtcgtt cggatggaag
     2161 atgcgggcca agatcgggga ccgcaagcgc tggtacgacc tgcccgaaga agtggacgga
     2221 ggaccgtaga tgatctctcg atttttcaac cgtgagcccg agacgcgcct tttcttcgcg
     2281 accgatattc acggctcgga gacgtgctgg cgcaagttcc tgaactctgg aaagcactac
     2341 gaggccaagg tcatggtcct cggcggcgac atgaccggca aggcgctcgt acccatagtc
     2401 gaggagagcg ctggccgctg gtacgcgacc ctgctcgaga accggaggga cttcaccacc
     2461 gaggacgagg tcaaggagtt cgaggactcg gtgcggcggc gcggatacta tccgttccgc
     2521 accgacccgg aggagatggc cgagctggag tcgaacgagc agctccgcca cgacctcttc
     2581 cacaaggaga tgctcggggg ggtcgagcgc tggatgcgga tggccgacga gaagctcgaa
     2641 ggcaccggca tcgagtgctt cgtcagcccc ggcaacgacg accagttcga ggtggacgag
     2701 atcatcgcga atgccaagcg cgtgaagctg gccgagggcc gggtgatgga gttcggagac
     2761 ttccagatgg tctctaccgg ctgggcaaac cgcaccccgt gggatacgta ccgggaggag
     2821 gacgagccgg acctcgccgc ccgcctgaag aagatgacct cgcaggtgac cgccgcgccg
     2881 gagaagacga tctacaactt ccattgcccg ccttaccact ccggcctgga tgaagctccg
     2941 gagatagacg cggacatgcg gcccaagcac ggcgggcgct cgatcgtgcc cgtcggctcg
     3001 acggcggttc gggaggcgat agaagaaggc cagcccgccc tggcgctgca cgggcacatc
     3061 cacgaggcgc ggggcaacac ccgcatcggg cggacgctgt gcatcaaccc cggaagctcc
     3121 tacgagcagg gccagctact cggggccgtc gtggacctcg acggcggcaa gaaagtcaag
     3181 cggttcgtac tcaccagcgg ataaggagag acagatggcg agcaacagta cggcgggttc
     3241 tagtacgagc gacctgttcc agcggaaggc gacgggtctc ctaaagggat ggtcggtgtg
     3301 ggacggcttc atctacgcgg cgttcgcgat caacctgatc acgctcggcc tctatatctt
     3361 ctcgttcgcg ccgttcatcc cggacggctc ccttatcttc gcgagagtcc tctcgggtct
     3421 ctacctggtc ttccaggccg tcacctacgc ctccctcata gcggtgatgc cgcgcgcggg
     3481 tggcgactac gtgtggatga gccgcgtgct cggcggcggc atagggttcg tcctcgcggt
     3541 aacgggctgg tggttcatac tctggcactg ggtcccgatc tacgcgaaca tcctcaacat
     3601 ccaggtcctc gtgccgctgg cgactatcgt gggtctcaag ggcgtggccg atttcttcgt
     3661 ctcgcccggc ggggtcttca ccgcctcgat gatcgtcgcg gtcatcgcct cggtcctcat
     3721 agcgctcggg atgcgcacct acgcccgcgt ccagaaattc tgtttctacg ggggcgtgat
     3781 agggctggcg atcatgctcg tcttgcttct cttccactcc aaggccgact tcatctcggc
     3841 cttcaacgcc aacgcgaagg agctgtacgg cgcgagcggc gacgcctacc agcagacgct
     3901 caaggcgggc gccgtgaagg gcgcgagccc cggcggcttc gtcttcggcc cgacgctctt
     3961 cctcataccg atgatcgtct tcttcaacct gtggtccaac tggggcgcga ccctctacgg
     4021 cgaggtgcgc ggggcgtcgg acttccgcaa gaacatctac gcgatgggtg gcgcgctcct
     4081 cgccacatcc ttcgtcgccg tcataatgct gctcctgttc gccaaaacct tcggctggga
     4141 cttctactac gcggccagca acgcctactg gtcgggcgcg aaggacgcgc cggtctcggc
     4201 ctggccctat ccgggtctgt tcgcggcgtt cttcttcgac agcccgatcc tgcagttcat
     4261 cctcgtcgcg ctgctctcgc tgtggttctt cgggtgggtc ggctcggtct tcctctcctc
     4321 tacgcgggtc gtgttcgcca cggcgttcga ccggatactt cccgagtggg tctccaagac
     4381 ctcgcgcaac ggcgtgccgt acgcggcgct ggcgctcatg ctcatacctt ccatcccgat
     4441 cagctacctg tacgccttcg gtgagaactt cgcgacctac acgctggacg cgacgctcgt
     4501 catcgcgatg accttcctgg gttcggcgat agcggcggcg gtgttgccgt ggcgcaagcc
     4561 ggagatctac aacgcctcgc ccatagcacg ctacaacgtg ctcggggtgc cgctcataac
     4621 cgcctccgcg gtgctgttct cggtgttcct gatcttctgc ctctaccagt ggctctcgga
     4681 tcccgtctac ggcatcaaca acgggcagtc cttgatctac atggtcgtcc tctacgtcgt
     4741 ggcgatagtc atgtacgtgg ccttccggct cgtccggcgc gcccagggca tggacctcaa
     4801 gatggtgtac gacgagatcc cggaggagta gctccgccag ccaaaatgtg ctgtttgggg
     4861 gatgcgattt cccctgtttg atgacatgat cctgaccatg ggatcaaggc atcggacgga
     4921 gaatgagcta tggcgttttt atggtctgtg tcgcgtaaaa gcggcgtaaa cgtgggcgag
     4981 acctcgcctt gctcgcgggc gggtagatca cccttcggga caggacgttc gaaccttctg
     5041 cccgctaccg cgtgaagact cgccttgaga ccgaaggtta atcagggggg agagacatgt
     5101 tcctatttgc tcattcgcac aagcccgtcc gtccccgcac cggtctccgc aagatgagga
     5161 tcctgatgag caacgagccc cgttcgtacc gggaaagcat cgcctctgta ctgcgggatc
     5221 tgcgtcccaa cgccgaggtg aagattgtgg agcaggagga gcttgagcgc tggatcctgc
     5281 ggttcgctcc agatctggcg atctgtaacc aggccacgta cgcggtacgg agcagggtcc
     5341 ccgtctgggt cgaactctat cccgagggga gggccgtgtc tacgatcagc atcaagggac
     5401 ggcgcgagag gtcggacggt attgacctct cccgcctcct ggagatagta gacttcgcag
     5461 aggttgtggc ggacgcgcga gaataattca cgagcattca ttcacgaatc atttatttac
     5521 gggtatttaa caaattagcc catttggttg atgccaggga tatactcacg ctctaatcta
     5581 ctgtccgatg acagctagag acgagatagc cttgatgata cgcacgagcg gcggatctgg
     5641 gacaagcttt ttcaggacgc ggaggatagt gtcgtctcga ccccgggcga gacgccatgg
     5701 tacgggtgat gaggttgagt gagtctgtcc gggcgcagag ccggctggag aaggtagctt
     5761 cgcaagagga ttcgttccac ggcggacgtt tcgcggcggc tttcgagcag gtggccgtgg
     5821 gcatggccaa gctgggtctg gatggcgggt tcctcagcgt caaccgtaag ttctgcgaga
     5881 tcgtcgggta ctcccggaat gagttactcg atagcgtggc ctatatggag atagtctgcc
     5941 gggaagatcg tgacgaccat cgacaggatt tccgcactct gctcgccgat ggcgaggaca
     6001 cttgttcggt ggagagaagg tgtgtccgaa gtggcggaga ggtcttctgg gccaacatta
     6061 cggcgtcgct cgtgcgcaac gacgaagaac gccccgcttt cttcgtcatc gtggtggagg
     6121 atatctccga gcgcaagcgg gccgaggagg cgctaaagga gagcgaggag ctctaccggg
     6181 cggtgatcga gcaggccgcc gagggcatct acctcttcga cccggaggcg atggagatcc
     6241 tggagtcaaa ccgcgcgttg caggagatgc tcggttactc cgccgaagaa ctcctgggga
     6301 taccggtgta caggctgata gttgacgagc gtaagagcat aaatgccaac gcgctgcggg
     6361 tgatactcaa cgacagctac tccttcggcg agcggaagta ccggcgcaag gacggttctc
     6421 tggtagatgt cgaggtaagt gggaccagga tcttctatgg cggcaaggag gcgatctacg
     6481 ctgttgtccg cgatgtcacg gagcgcaagg aagtggagga gaacctccgc cagagcctga
     6541 gcgtgctgct cgctctgcgg gaggcgggcc agatactagg atccaccctg gagctggagg
     6601 agatcggttc caggctgctg gagatcatga agaacgtctc gaacctcact acgacagtga
     6661 tcagcgtcca ggaggacaac gggcagttgc gtatctggcg ctcggtcggc ctagaaagcc
     6721 tgtggagcgg ggcgcggttc ttcccggcgg cggaggaggc gcgccgggta gcgatggagg
     6781 aggaagagcc ccggctgttc aggctcaaac cgccgggcgc atcgagaagc agtgtcatgg
     6841 gcctgtgtct gccgctgcgg atgcgggacc atgtcttcgg cgtccttgag gcttacggtc
     6901 ccgactcgct ggcggagagc gacacgatgg aggtcttgcg cagcatcacc aatcaggctg
     6961 cgagcgccct ggagaacgcc cgcctgtacg gagagctcgc ggagcgcgag cgccgcctgc
     7021 aggagttgat cggcaagctg atcgttgctc aggaggagga gcggcatcgc gtcgcgtacg
     7081 aggtgcacga tgggctggct caggtagcgg tcgccgcgca ccagcacctg caagccttcg
     7141 ccgaacggca tcctcccgag atggatcacg cccgggcgga tctccaggta gtgctcaagc
     7201 tcgtgcgccg cacggtctcc gacgcgcgac gtatcatcgc caacctgagg ccgacggtgc
     7261 tggacgacct ggggctcgcg gcagccctgg cgctggaagt agagcagatg gccgatgaag
     7321 gctacgaggt tgaataccac gaggctctcg ggggcgagcg aatacccgga ccaatagaga
     7381 tcgctttctt ccgcgtcgct caggagtcac tgaccaacat gcgcaagcat gcccggaccc
     7441 ggtgggcgag gatcgacctc gaacgccggg accatcaggc gcggctggtc gtcagggatg
     7501 ccggccgcgg gttcgacccg agatcggagt tcgccagcag cggtccgggc gagcgcgtag
     7561 gtctggccgg gatgcgggag aggatcggat ctctcggcgg caggctagag atcgacgcga
     7621 gacccggtgt cgggacctcg ataatcgcga ccgtcccgct ggaggcctga gatgaggctc
     7681 ttcgcgcacc gggaccatga tgcgaacgga ccaccggcat ggccgtagat cccgttcgcg
     7741 caagatattg gagcaccaaa accctaatct ccagccgaag cagcaataga aggagtcgcc
     7801 ttgagaaata cgaagcttcc caccagtaaa ttgagcaccg ccgcccgcct catcatagtc
     7861 gacgatcatg atatggcgcg cgagggactg cgggatatgc tggccgacgt cccggacatc
     7921 gaggtgatcg gcgaggcatc caacgggaag gaggcgctcg tactctgttc gatgctgcgg
     7981 ccagatctcg ctctcatgga cgtgaggatg ccggagatgg atggcctgtt ggcgaccagc
     8041 aagataaagc agagccatcc cgagatcagc atcctcatgg tgaccatgca cgagaacccc
     8101 gagtacctat tcgaggcgct caaggccggc gcggcggggt acgtgctaaa ggacgcttcc
     8161 cgccaggagg tcatcgacgc cgtgagacag gtgcgtctcg gcgagtcgcc gttagacccg
     8221 gaccttgcgt cccggttgct gcgcaggatc gccgccgaaa gcacgccggg acgcaggctc
     8281 tccggcggcg agaaccgcca tcggttcgac aagctgaccc gtcgtgagct ggaggtgctc
     8341 ggtctgctca aggagggtat gaccaaccgg cagatcgccg gcgtcctctt cgtgagcccg
     8401 gggaccgcca agaaccacgt ggaacacata attacgaagc tgggagtctc cgaccgtaca
     8461 caggcggtgg tgagggccct cgaaatcggt attatcgact tcaactcctg aatgagctac
     8521 cggctataag ccgagaaata tgccgcttgg ccgatgtaac cgtcggcgtc cgcgcgcatc
     8581 ataaacctcg atgagcactc ctttgattga aggatgagaa agattttcgc acagattcag
     8641 gagggggcca cgaaccaggg taagggcacg ctggccgtct gtggagatcc agtggccggg
     8701 agagccctgg ttcttctact gcgtggcgca gattacgacg tcaagtttct ccctctctcg
     8761 gtcctggaag agccgggaat gctggagggg gtgcagttgc tgatcctcgg gcttacgccg
     8821 ggactcaacg aggaagggcg cggcgctttg ttgaagtttc tcagaagcag cccgttcgca
     8881 gcctcgatgc cgaccctgga gttggttgtg cccgaagggg ctcagaatgc ggaaccgcgg
     8941 gactcgcgcc tggtggtccc ctggccttgc agcacggaac agttgaagga gcgcatcgag
     9001 accgctctac ggaccagact ctagctcctc ctgaccggat gctttacggc gcgctcgcaa
     9061 cgcgctcgca atagctaccc ggcctcgctg acctcagaga tacggcccgg agatacggcg
     9121 caacggcgcg cgaacgtcta tcggatgaga cctcgcgcgc gcccgctttc gcccgcgccg
     9181 acagtcgcgc tacgagtcgc gccacgaggg ggatcgggaa atgtgccacc tggccgatgc
     9241 tgccggatga cgccctatct aacatcacat taacaaatcc ttgaccccaa ccagaaggaa
     9301 gtagtagccg tgtcaagcat catggaggcc gggatcaggc agtcggctcc gacaccgggg
     9361 tcttattcag tggcagcgag gagccagctc aagagagcag taggtcaggc ggtagtggta
     9421 cccgccctga tcctctccga cgtggtgctt gcgactgcgg tctgggagat agttttcgcg
     9481 gttcagcagg tcttcgggca tgctcaatta tcgattgtat cggtcgtggg tttcttccct
     9541 tacgttgctg cgtgggtggg actccgcgcg ttatctggtc tgtatcccgg ctacgggatg
     9601 ggacagatag aggagttgcg gcggcagacg ctggccctgc tcaccacgct ggctattacc
     9661 gcggtcttcg cgctcgcttc tcaggctgag gacgtgctat cacgtctgat gctcttcggg
     9721 tgttctctcg ggctgttgct ggtcgcaccc ctggtgcgtt acggcgtcaa gcacgtgctg
     9781 atgaggacgg gcacctgggg gaagccggtc gtggtgctcg gcgctcacga ggcgggagcc
     9841 cgcatgttga gagccctgcg gcgggagtgg caactcggtc tcaagcccgt cggtgtcttc
     9901 gataaccacc gagtcccggc ggggggcgag atcgagggta tcccttacgg cggaaccctg
     9961 accgacgccc tgtccgcggc cggacagcta ggtatagaga cggccatctt cgcgatgccc
    10021 tacacgcggc gcgagagcct ggcgaagctc gt
//
LOCUS       DASSMB010000209         5172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1317229, whole genome shotgun sequence.
ACCESSION   DASSMB010000209 DASSMB010000000
VERSION     DASSMB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317229"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1634)
                     /locus_tag="VFJ72_12500"
     CDS             complement(279..1634)
                     /locus_tag="VFJ72_12500"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HET7480327.1"
                     /translation="MVPGKRELEDRVHEWRPGGYVVLFACLLLTVLLTGCGGDQSSRG
                     ESHAGNPAQARVADGGLPAETRLEKTPETRKRNVVLIVLEATRARSVTPYNPDLATMP
                     FLDELSKKSLLAKRAYTVVPHTTKALVASECGREPHLVRKITESQPDNLPFQCLPKLL
                     GEQGYKSVWFQNGAGNFEGRTQLVENFGYDEFYSLEDMDPDGFERVAFGREENMMLDP
                     SRQWLEQNGKTSFLATYKTVAPHSHYINPDRYGVVDFTEKEKLNRYLNCIRYVDFFLK
                     NLFDQYKKLGLYDDTIFIIYGDHGEGFGEHGRYQHDNVIYEEGLQIPLIVHDPERWQS
                     GERVDGLANQLDILPTIFDLLNYRVEGGDYPGYSLLDLPEDRTLMFNCWYDKRCLAST
                     HGNEKYIYHYGNRPEQLFDLEKDPLEKTNLADSRKKDLEARRDALLKWRSSVNAMYEQ
                     K"
     gene            1718..2389
                     /locus_tag="VFJ72_12505"
     CDS             1718..2389
                     /locus_tag="VFJ72_12505"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET7480328.1"
                     /translation="MWTKFVTERRLEFGGHTDPAWTNGHALSASLMVPVDAPSLHERL
                     EPMRDALRPFPFVSLHPDHFMHITLFLLGFVVPEPGEDDEVSPERLAEIEAASRRALA
                     DIPTFEVELANLNAFPAAAFVEVHDGGMLERVREALRGECGLREPPGPPHLTLAYFQP
                     SREMRAPADLISALEKFRDWPVGEVVVGEVELTLLDLHQDYPEPKPLARIPLAEPAGA
                     SSALT"
     gene            complement(2382..2894)
                     /locus_tag="VFJ72_12510"
     CDS             complement(2382..2894)
                     /locus_tag="VFJ72_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7480329.1"
                     /translation="MDLYLVRHAVAYERDSERWPDDAERPLTPRGEEDFREAARGVEQ
                     IVPEVRALLSSPYSRAWRTAEILAELEGWPEPKSFPALEPEVPPEKVALALQTYSEAE
                     SVVLVGHRPGLHELAVYLLTGDAGGADIKIKKGGAIRITFDGAPQPGAGRLHWLLTPK
                     VLRALRRTVT"
     gene            complement(2904..4550)
                     /locus_tag="VFJ72_12515"
     CDS             complement(2904..4550)
                     /locus_tag="VFJ72_12515"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET7480330.1"
                     /translation="MRERVSDHHEVEWQFDALDVRPVARWLESRSHWDGPAVAANETR
                     DLTDTYFDTGDWRLYRAGYALRVRQKTGAGGGFEATMKSLDAGEDGVRRRREISEPVS
                     GAGADDLLAAPGPVGVRLRALAGSVSLAPLFSIKTRRAAYAISLYGEGVGEVVLDTSE
                     IPLEDGAEPVMLRRVEVEMQPETEPEAVDGVRESLESFVGELREGCRLSPARASKYEA
                     AVFAHNLTPPGALDLGSTAVDESLTVGQVAFAVMREQFVEFLAHEPGTRIGEDPEELH
                     DMRVASRRLRAAVKVFDEALPVRLRGMEAEVKWIATALGEVRDLDVQLEQLEEWLAGA
                     EPEDREPLGELRGVLEDRHEKARRAMIRALDSRRYARLVESLELLLRRGPSPRSKAAG
                     RPVLAVAPDLVRKRYRKVRKAGDRISEESSAADYHDLRKKGKRLRYALEFLSGVYGDA
                     AGDLVSPLKKLQDVLGDHQDAEVAIDHLRELSDARAGRRRLSPRTVFVMGGIAHRYAL
                     QKAELRAQFPKTYKGIKGKPWKKLRTVMDNLRPSEDEKTS"
     gene            4765..>5172
                     /locus_tag="VFJ72_12520"
     CDS             4765..>5172
                     /locus_tag="VFJ72_12520"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET7480331.1"
                     /translation="MAELTISYWEVLIRLLATVVLCGAIGFERETRDQSAGFRTHILL
                     GLGAALFTLVSSYGFEAFTRAALESGGRGIQFDPTRIAAQVVTGIGFLGAGAIIRQGI
                     DVRGLTTAASLWAAAAIGMASGAGYYFGAAATTV"
BASE COUNT          823 a         1725 c         1649 g          975 t
ORIGIN      
        1 ctgcaggtct ctatatatga cctgtgggtg ttgggaccga cggggaagga cacggtaagt
       61 ttacagacgt gcatcaaccc gccggcatat gatgtgcggc tgatagttag gggagacctc
      121 accagcatag accccgcctg agagtaagaa cccgaaagct aaacgtccga gcgtcccttc
      181 caggggcgct cttcttttgt aactccctac tccaacggtc agacagctac agcaacggcc
      241 atctcgcgca tgaggcagcg ccctgtccgt ctactttttt acttttgctc gtacatcgcg
      301 tttaccgagg agcgccactt caacaaggcg tcgcggcggg cttcgaggtc tttcttgcgc
      361 gagtcggcga ggttggtttt ctccaggggg tctttttcga ggtcgaatag ctgttccggg
      421 cggtttccat agtggtagat gtacttctcg ttgccgtggg tgctggcgag gcagcgtttg
      481 tcgtaccagc agttgaacat cagggtgcgg tcttcgggaa ggtcaaggag cgagtagcca
      541 gggtaatcgc cgccttcgac tctgtagttc aggaggtcga agatggtcgg caggatgtct
      601 agctggttgg cgagcccatc cacgcgctcg ccgctctgcc agcgttcggg gtcgtggact
      661 atcaggggga tctgtaatcc ctcctcgtag atcacgttgt cgtgctggta gcggccgtgc
      721 tctccgaaac cctcgccgtg gtcgccgtag ataataaaga tggtgtcgtc gtagaggccc
      781 agtttcttgt actggtcgaa gaggttcttg aggaagaagt ccacatagcg gatgcagttg
      841 aggtagcggt tcagcttctc cttctcggtg aagtccacca ctccgtagcg gtcggggttt
      901 atgtagtgag agtgaggagc gactgtcttg taggtggcga ggaaggaggt tttgccgttc
      961 tgttcgagcc actggcggct gggatccagc atcatgttct cctcgcggcc gaaggcgacc
     1021 cgctcgaaac cgtccgggtc catgtcctcc agcgagtaga actcatcgta gccgaagttc
     1081 tcgacgagct gtgtgcgacc ctcgaagttg cccgcgccgt tctggaacca cacgctcttg
     1141 tagccctgct ctccgagtag cttcggcagg cattggaacg gcaggttgtc cggctgggac
     1201 tccgtgatct tgcgtaccag gtgcggctcc cggccgcact cgctcgccac gagcgccttg
     1261 gtggtgtgcg gcacaaccgt atatgctcgc ttggcgagca ggctcttctt cgagagctcg
     1321 tccaggaacg gcatggtggc gaggtccggg ttgtagggcg tgacggagcg ggcgcgggtc
     1381 gcctccagca cgatcaagac cacgttccgc ttcctggtct ccggcgtctt ctccagcctc
     1441 gtctcggctg gcaaaccccc gtccgccacc cgcgcctgcg ccgggttgcc ggcatggctc
     1501 tctccgcgcg aggattgatc tcccccgcac cccgtgagca acactgtcag caggaggcac
     1561 gcgaagagga caacatatcc tcctggccgc cactcgtgca ccctgtcctc caattcgcgt
     1621 ttgccgggaa ccatgatagt gctccttcgc gccgtccgtc caccggcggc cgtcttgcgg
     1681 gatctgttac agttccggga tggatggata tgcggaggtg tggacgaagt tcgtcaccga
     1741 gcgacgtctg gaattcggcg gacacacgga cccggcctgg acgaacggac acgccctctc
     1801 tgcttcgctg atggtaccgg tggacgcacc gagcctgcac gagcgcctgg agccgatgcg
     1861 ggacgctctg cggccctttc cgttcgtctc gctgcacccg gaccacttca tgcacataac
     1921 gctcttccta ctcgggttcg tggtccccga accgggcgaa gacgacgagg tctcgccgga
     1981 acgtctggcg gagatagagg ccgcgtcccg gcgggctctc gcggacatcc cgacgttcga
     2041 ggtggaactc gccaacctca acgctttccc ggccgccgcg ttcgtcgagg tccacgacgg
     2101 cgggatgctc gagcgggtac gcgaggcgct gcgcggcgag tgcggtctcc gggagccgcc
     2161 cggaccgccg cacctgacgc tcgcctactt ccagccctcg cgtgaaatgc gtgccccggc
     2221 ggacctcatc tccgcgctgg agaagttccg cgactggccg gtgggagaag tagtggtcgg
     2281 cgaggtggag ctaacgctgc tggatctaca ccaggactac ccggagccga agccgctggc
     2341 ccggataccg ctcgcggagc cggcgggcgc cagttcggcg ctcacgtaac cgtccggcgc
     2401 agggcgcgca ggaccttcgg cgtgaggagc cagtgaagcc tgccggcgcc aggctgcgga
     2461 gcgccatcga aggtgattcg gattgctcca ccttttttga tctttatatc cgcgcccccg
     2521 gcgtcgcccg tgagtaggta gaccgcgagc tcgtgaagac cgggccggtg gcctacaagg
     2581 accacggact ccgcctccga gtaggtctgt agggccagcg cgaccttctc gggcggcacc
     2641 tcgggctcca gggccgggaa ggattttggt tccggccacc cttcgagctc cgccaggatc
     2701 tccgccgtgc gccaagcgcg ggagtaagga ctgctcagga gcgcccgcac ctccgggact
     2761 atctgctcca ccccgcgagc cgcctcccgg aaatcctcct cgccccgcgg cgtgagcggc
     2821 cgctccgcgt cgtccggcca gcgctccgag tcgcgctcgt aggctacggc gtggcgcacc
     2881 aggtagagat ccaaccttcc ccctcagctc gtcttttcgt cttcggacgg acgcagatta
     2941 tccattaccg tacgcagctt tttccagggt ttgcccttta tgcctttgta ggtcttcggg
     3001 aactgcgctc gaagctccgc cttttgcagc gcgtagcggt gtgcgatccc gcccatcacg
     3061 aagaccgtgc gaggcgataa cctgcgccgc cccgcgcggg cgtcgctcaa ctcccgcagg
     3121 tgatcgatgg ctacctcggc gtcctgatgg tctcccagca cgtcctgcaa tttcttgagc
     3181 ggcgacacga ggtccccggc cgcatcgcca tagacgccgg agaggaactc cagggcgtag
     3241 cgcaggcgct tgcccttctt ccgcaggtcg tggtaatcag cggcggacga ctcctcagag
     3301 atacggtccc cggccttgcg gaccttgcgg tacctcttgc gtacgaggtc cggcgcgacg
     3361 gcgagcaccg gccgtcccgc ggccttcgag cgcggcgacg ggccgcgcct cagtaagagt
     3421 tcgagcgact cgaccagccg agcgtagcgc cgggaatcga gggcgcggat catcgcccgg
     3481 cgcgccttct cgtggcgatc ttcgaggacg ccgcggagct ctccgagcgg ctcccggtct
     3541 tcgggttcgg ccccggcgag ccactcctcc agttgctcca gttgcacgtc cagatctcgc
     3601 acctctccga gagcggtggc gatccacttt acctccgcct ccatgccccg caggcgcacc
     3661 ggcagcgcct cgtcgaagac cttgaccgcc gcccgcagcc tcctcgatgc gacccgcatg
     3721 tcgtgcaact cctccgggtc ctccccgatc ctcgtgcccg gctcgtgggc caggaactcg
     3781 acgaactgct cgcgcatgac ggcgaaggcg acctggccga cggttagcga ctcatccacg
     3841 gcggtggagc cgagatccaa cgcccccggc ggcgtgagat tatgggcgaa gaccgccgcc
     3901 tcgtacttgg atgcccgcgc gggcgagagc ctgcaaccct cgcgcaactc tcccacgaac
     3961 gattcaaggc tctcccgcac cccgtccacc gcctccggct ccgtctccgg ctgcatctcg
     4021 acctcgaccc gccgcagcat aacgggctcc gcaccgtcct ccaacggtat ctccgaggtg
     4081 tcgagcacca cctcccccac accctcgccg tacagcgaga tggcgtaggc ggcgcggcgg
     4141 gtctttatgg agaagagagg cgccagagac accgacccgg cgagcgcgcg caaccggaca
     4201 cccacgggac cgggtgccgc cagcaggtcg tcagccccgg cgcccgagac gggctccgag
     4261 atctcacgcc gccgcctgac accgtcttcc cccgcatcga gcgacttcat cgtcgcctcg
     4321 aagcccccgc cggcgcccgt cttctgacgg acccgaaggg cgtaaccggc gcggtagagt
     4381 ctccagtccc cggtgtcgaa gtaggtgtcg gtcaggtcac gggtctcgtt cgcggcaacc
     4441 gccggaccgt cccagtgaga gcggctctcc agccagcgag ccaccggacg cacgtccagg
     4501 gcgtcgaact gccactcgac ctcgtgatgg tccgaaaccc gctctcgcac gcccgtcgaa
     4561 aacctccgtc tgacccggtc cctgatggtc ccggaaaggt cgttatcgta gcagttgtgt
     4621 ctatcctggt caatcttcca cgtggcccag cttatgcatg gccgcggtcc atcatgtgca
     4681 gatagcgggc ggttccagac ggtacgattt gaaaggggct ccggcggtct atatattcgc
     4741 ggagtacgag agggaggtgc gagcgtggcc gaattgacca tctcttactg ggaggtcctg
     4801 atccggctcc ttgcgacggt ggtgttgtgc ggggcgatcg ggttcgagcg cgagacccgc
     4861 gaccagtctg cggggttccg cacgcacatc ctgctcgggc tcggagccgc gctcttcacg
     4921 ctggtctcgt cctacgggtt cgaggctttc acgcgggcgg cgctggagtc gggcgggcgc
     4981 ggcatacagt tcgacccgac cagaatagcc gcccaggtcg tcacgggcat cggtttcctg
     5041 ggtgcgggcg cgatcatccg ccaggggata gacgttcggg ggctcaccac ggcggcgagc
     5101 ctctgggccg ccgcagcgat aggcatggct tccggcgccg ggtactattt cggcgccgcg
     5161 gccacaaccg tc
//
LOCUS       DASSMB010000210        15086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_173424, whole genome shotgun sequence.
ACCESSION   DASSMB010000210 DASSMB010000000
VERSION     DASSMB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15086
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173424"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="VFJ72_12525"
     CDS             complement(<1..1348)
                     /locus_tag="VFJ72_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7480332.1"
                     /translation="MSPVQVESYPAIIGGEKVNGSRTFEVLDPSTGEPFAEVARCGEA
                     EIDQAVAAAREAHESTWRRTTPAERARILHKISELLRRDHEELARMESLDTGKPLKQA
                     KADATVAARYFEFYANTIESFYGDTIPAMADTFVYTLREPFGVTAHIIPWNYPIQIGC
                     RSVAPALAVGNCCVMKPAEEAPLTPIKLGELALEAGLPPGALNIVPGFGEEAGAPLAA
                     HTGIDHVAFTGSVEVGQLVSKAAADNVIPVTLELGGKSPNVVFADADIESAVPVIVNS
                     IIQNAGQTCAAGSRLLVEGRVQDQVVEAVAKRFGEIKIGPGLEDPDLGPIISREQLER
                     VNSYVQAGKDEAELLAGGDTPRDDRLRGGFFFEPTLFGGVSPGDRLAQEEVFGPVLVA
                     SSFDDLEEAAQIANNTEYGLVAAVWTRDVGKAHWLAREIRSGQVFVNTYGAGGGVEM"
     gene            complement(1401..2012)
                     /locus_tag="VFJ72_12530"
     CDS             complement(1401..2012)
                     /locus_tag="VFJ72_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HET7480333.1"
                     /translation="MRLHSAPMSGNGYKVRLLLGLLGEECEVVDYNTKGGETHTPEFL
                     KNVNPDGKVPALELDDGTMLPESGAIMLYFAEGTPYLPDDRLERAQVMRWMFFEQYSL
                     LLYLSRPRLWRMWEVEMTEAREAQLEEFFEQGYRALGVMERHLADREFFVGDVPTTAD
                     VALYVYPRVCHEGGYDLEGFPGVRAWMERIEALSGYVPPPGRP"
     gene            complement(2143..2709)
                     /locus_tag="VFJ72_12535"
     CDS             complement(2143..2709)
                     /locus_tag="VFJ72_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HET7480334.1"
                     /translation="MAGEIRLEAPLTREGNESLKTGDVVLLGGVLYTARDAAHARIKE
                     AIERGEELPFDPDGQIVYFTGPAPARPGHPIGPAGPTTASRMDPYSPLLIERGLKGMV
                     GKGVRSQAVRESMREHGCVYFGAIEGTAALLADCVKEAEVVAYEDLGAEAIRRLVVED
                     FPVIVVNDLHGGDLYTEGRARWRRTARR"
     gene            complement(2713..3555)
                     /locus_tag="VFJ72_12540"
     CDS             complement(2713..3555)
                     /locus_tag="VFJ72_12540"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HET7480335.1"
                     /translation="MREISAETLVETVRDLCIEANTELPQSHLAAMRRALGDEESPHG
                     REVLERLLQNAEVARERCVAFCQDTGYAVFFVEMGSEVRIAGGEISAAIDEGVRRGYE
                     EGYLRKSIVRSPVDRVNTGDNTPAIVYYDPVPGDGFKLTMLVKGAGCDNMSALKMLTP
                     AEGVEAMKDFIVETIEKAGPNASPPVTVGVGIGGPFEKAAMLAKKALTRPSGEPSPDP
                     KLAQLEREILDEINATGIGPAGYGGTQTALCVHIESFPTHIAAFPVAVNLDCHSHRTA
                     SREL"
     gene            complement(3585..4355)
                     /locus_tag="VFJ72_12545"
     CDS             complement(3585..4355)
                     /locus_tag="VFJ72_12545"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7480336.1"
                     /translation="MTVRDGRLEGKVAIVTGAASGIGRASVERFAAEGARVVVADLDA
                     DRAEEVARGISSRGGEAVHVRCDVTRAEDARAMVRAAVEHYGGLDILFNNAGFTNKPR
                     PGEETTEEDFDRALAVNVKGVFLCSQAAIPALREGGGSIIVTASVMGIRTRPGFTAYA
                     ASKAAAIHLARTLALELAEDGIRVNCLAPVATDTPMLGSFIGDRDPEEGRAAFISSIP
                     LGRLARPEDVAAAALYLASDDSEFITGTVLPVDGGRGI"
     gene            complement(4490..5419)
                     /locus_tag="VFJ72_12550"
     CDS             complement(4490..5419)
                     /locus_tag="VFJ72_12550"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HET7480337.1"
                     /translation="MVLAVFAKVLLPILVVVGLGYLLRRRFDLDVQSINRVSIYLLSP
                     ALIFTSLTRVRLAADETLRITAFMVLFVLVMGVLTWTMSRTLRLDAMTTSALLLCVMF
                     MNAGNYGLPLAQFAFGEEGLRRAVLFFVVQAVLAQTVAVYIAGAGHGDWLDGFKRLFR
                     MPQVYAVLAALAVRWTGIQLDASSGGILNDLFRGVALMGDATVPVLLIVLGLQLAESN
                     KTTQRREIALATVMRLVVSIPLALALAYLLQMDGLSTKLAVILASMPTAVNMMILAIE
                     FDVRPRLVSGVVAVSTFLSLISLTVLLLIFGVS"
     gene            5909..6238
                     /locus_tag="VFJ72_12555"
     CDS             5909..6238
                     /locus_tag="VFJ72_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480338.1"
                     /translation="MPELVRGPYVRVDEVCSLGKRAEDFVVVPFPIQEMQLLRHCTPA
                     GAVHFVRAYSACEWEKVRRIRAHEIAVEVLDPGSAKQVSGTGIREALRDGSPLEHLVP
                     TGSVGAK"
     gene            complement(6283..7464)
                     /locus_tag="VFJ72_12560"
     CDS             complement(6283..7464)
                     /locus_tag="VFJ72_12560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7480339.1"
                     /translation="MEKSAPYMEEIVSLRRHLHQHPELSGEERATSRTVQQKLEERGI
                     PFTTGYAGTGVLGLIEGGKPGGTVALRADIDALPIHEENGHDFVSKNEGVMHACGHDA
                     HTAMLVGAAWLLNESKEELPGRVLLVFQPAEEASPTGGARPMMKDGVFREHRPDVIFA
                     QHVWPELPVGRIGVRRGAMTGASDRFKVTIEGVGGHASKPHQTTDAIVVANQVINALQ
                     TVVSREVNPLESAVLTIGKIRGGTRYNAIADRVTLEGTVRTFRPETKETVKDRFHSIV
                     TGVTEAMGATARIQYRDGYPAAINDPVWAERVAETARDLLGDDATPEIEPSLGGEDFG
                     RFLLKYPGAYFRLGTARSDDREKKRLHDSRFDIEESALQIGVELLARIAADSLCQLES
                     R"
     gene            complement(7616..9601)
                     /locus_tag="VFJ72_12565"
     CDS             complement(7616..9601)
                     /locus_tag="VFJ72_12565"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET7480340.1"
                     /translation="MAENLVTKIFKEHMVHGDYEPGEEVGLRIDQTLLQDATGTMASL
                     QFERMGVDRARVDLAVQYVDHNIIQLDFKNPDDHRFLQAFAAKYGVYYSRPGNGICHY
                     LHVERFARPGATLVGADSHTTSSGALGSIAIGAGGLDVALVMAGQPFEIEVPKVVGVE
                     LTGKLKDWVTPKDVILELLRRRGVRGGLGRIFEFYGEGVESIDVTGRTTICNMIAELG
                     ATTGVFPSDERTREWLEAQQRTDDFRELAADEGADYDEYEHIELDKLEPLIAQPHSPG
                     NVVPVSEVAGTKAAQVCLGSSVNSGFEDLAIAAAVLRDETVYPGLVLTVTPGSRQILD
                     SIAQTGVYSDLVRAGARMLEPACGPCVGMGQAPPSDSVSVRTFNRNFPGRSGTMTDRV
                     YLSSPTTAVATALRGVITDPRELGDYPELPEAPANPAVDDRQILAPAPEEEASGIEIP
                     RGPNIKPPPEAPELPDSLECAVTIVVEDDISTGDLAPDGVEVMSFRSNVPEIARFTFR
                     RFDPDYHKKAQEMAPGFIVGGHNYGQGSSREHAALAPLHLGIRAVIAKSFARIHRRNL
                     ISQGILALRFKDEDDYDKFEQGQKWELPEIRSHLESGDTEVPLKGEGGEVTLLAEFSR
                     RERDILLAGGVLRKLRDESREDEQPESPQRVSGEAER"
     gene            complement(9779..10717)
                     /locus_tag="VFJ72_12570"
     CDS             complement(9779..10717)
                     /locus_tag="VFJ72_12570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480341.1"
                     /translation="MDDLAERGFTGVLHTFSENDLYYYRDQMRRIVEVSHAAGLEVQI
                     NPWAVGHAFGGESESLFTASHPEVGQIFDDVTPVGAACPNHPAFRAFVRSWADAAIET
                     GADRVFWDEPHWADPSRFGVSTDRWACRCERCRGAFFEQYGERMPHEMTPEVAAFREA
                     SLVGFVRELVAHVETRGSRSTVCLLPLTEGPLGLSDWSEVARMPGLDTLATDPYWKTF
                     GHPARDFVGGFASRVRDLGAEHGVSAQVWIQGFGLGPEDGDDLHAAVQAARSAGVEDL
                     WTWGYEACGHMTHLGTREPERVWATLTEALTGYQSG"
     gene            10974..11198
                     /locus_tag="VFJ72_12575"
     CDS             10974..11198
                     /locus_tag="VFJ72_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET7480342.1"
                     /translation="MSTSFPAIRFHDLRHSCLSLLAQRGEPIRDSQALAGHATDAFTL
                     QRYTHHYDASARRTADAMEDILPDGIPDGP"
     gene            complement(11300..11372)
                     /locus_tag="VFJ72_12580"
     tRNA            complement(11300..11372)
                     /locus_tag="VFJ72_12580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11337..11339),aa:Thr,seq:cgt)
     gene            11504..11893
                     /locus_tag="VFJ72_12585"
     CDS             11504..11893
                     /locus_tag="VFJ72_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET7480343.1"
                     /translation="MQRVMGVSREAVIQALDNCYDPCCRERRISVVDIGLIESIEIQD
                     RSVKIEMVLTTGWCPFASRLLEMVEEEVGGLPEVDNVEVEVVWDPTWTPERMSQGARE
                     KLRLPLEQLAPLREARLREARIRGERA"
     gene            11890..12897
                     /locus_tag="VFJ72_12590"
     CDS             11890..12897
                     /locus_tag="VFJ72_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7480344.1"
                     /translation="MINDDVLVFDCVAHPFNFDPSNVLGNAGELFRQHLFAFHNVLTP
                     ESEPKLSSDEFLKEWSTAEISRMVFDESDTDMLVAMPLPLTDLFHDGLSPWERCAELR
                     DRNPERTVFWGTVNPLEGKRALDQMEVQVKEYGAKAFKFYNVRYDYGEPFPWRMDDPR
                     VAFPIYEKAQELGINLIGVHKGVPLGPQPIEATQTWDMDGAAANFPDINFVIFHVGLP
                     FIDETCWQLVRFPNLYASIAATINFVVRSPRQFAEWIGKLMFWCGEDKIIYGSETPIW
                     HPKWALDAFWDFEIPQDLVEGYGYPQLTEQAKRKILGENLLRLSGMDVEETKQRLNGA
                     A"
     gene            complement(12940..13359)
                     /locus_tag="VFJ72_12595"
     CDS             complement(12940..13359)
                     /locus_tag="VFJ72_12595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7480345.1"
                     /translation="MTTRYIHTCYRILDPERSKDFYVGKLGMKLVGEMHFDTAANYFF
                     AMEPDASQPMLELTHNHDQTEPYDVGNGYSHVAFTVDDLEGTVAKLEQDGVEVSLAPK
                     TMTVEGHDYRIAFVVDPDGYRIELIERGTMKVGDIIQ"
     gene            complement(13667..14503)
                     /locus_tag="VFJ72_12600"
     CDS             complement(13667..14503)
                     /locus_tag="VFJ72_12600"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7480346.1"
                     /translation="MGEDRGVRLERRLSMLDVPPLDRDGGRGGAKFESIRRVFRILDL
                     VSRREGLTAKLLARDLGVSLSTCYYLVNILIEEGYLEKDPTRKGYRIGAAISTLHERR
                     SGTDLGSAAEPVLEELAQRSGRHAYLGVLSDGAVTVSEVKSPCKSPPVGLVRGFHGAS
                     HALALGKVLIAGTGAEGVEDYAEGFGLEQFTTRTIVQPGLFKVHLDRVRAEGVATDVE
                     EFEANLCCVAAPILGSSGEIEGAVGVSTSARRYASEAERMIELVQWAAQETSSLLREG
                     EG"
     gene            14817..>15086
                     /locus_tag="VFJ72_12605"
     CDS             14817..>15086
                     /locus_tag="VFJ72_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7480347.1"
                     /translation="MYEKDGEKYFVVDEHLHFWDASPENTTNKYGEGFIKCFYDYHAN
                     LSPDEYTWTFEKYRKYSEEDMMHDLFEVGYVDKAILQSTYLTDFYD"
BASE COUNT         2640 a         5137 c         4559 g         2750 t
ORIGIN      
        1 gcatctcgac accgccgccc gcgccgtagg tattgacgaa cacctggccg gaccggatct
       61 cccgggccag ccagtgcgct ttcccgacgt cccgcgtcca gaccgcggcc acgaggccgt
      121 actccgtgtt gttggctatc tgcgccgcct cttccaaatc gtcgaaggac gacgcgacca
      181 gcacgggacc gaagacctcc tcctgggcca gcctgtctcc gggcgagacg ccgccgaaca
      241 gggtgggctc gaagaagaag ccccctctca gccggtcgtc gcgcggggtg tcgccgccag
      301 cgagcagctc cgcctcgtcc ttgcccgcct ggacgtagct gttcacgcgt tccagttgct
      361 ccctggagat gatcggcccg aggtcggggt cttcgaggcc cggtccgatc ttgatctccc
      421 cgaacctctt cgcgacggct tcgacgactt gatcttgcac ccttccctca accagcagcc
      481 gcgagccggc ggcgcaggtc tgtccggcgt tctggatgat cgagttaact atgaccggga
      541 ccgcgctctc gatgtccgcg tcggcgaaga cgacgttcgg ggacttgccg ccgagctcca
      601 gcgtcaccgg gatgacgttg tcggcggcgg ccttgctgac gagctggccc acctcgaccg
      661 agcccgtgaa ggccacgtgg tctatgcccg tgtgcgcggc gagcggcgct cccgcctctt
      721 caccgaatcc gggcacgatg ttgagggcgc cgggcggcag gcccgcctcc agcgccagct
      781 cgccgagctt tatcggggtg agcggcgctt cctcggcggg cttcatcacg cagcagttgc
      841 ccaccgccag cgccggggcc accgagcggc agccgatctg gatcgggtag ttccacggga
      901 tgatgtgcgc cgtcaccccg aatggctcgc gcagcgtgta cacgaatgtg tccgccatcg
      961 ccggtatcgt gtcgccgtag aacgactcga tcgtgttggc gtagaactcg aagtagcgcg
     1021 ccgccaccgt cgcgtcggcc ttcgcctgtt tcaagggctt gccggtgtcc aggctctcca
     1081 tccgcgccag ctcctcgtgg tcgcggcgca gcaactcgga gatcttgtgg agaatcctgg
     1141 ctcgttcagc cggggtggtg cgccgccacg tgctttcgtg cgcctcgcgc gccgccgcga
     1201 ccgcctggtc tatctcggct tctccgcacc gagcgacctc cgcgaacggc tcgcccgtcg
     1261 acggatcgag gacctcgaac gttcggcttc cattaacctt ctcaccgccg atgatggcgg
     1321 ggtagctctc tacctgtacc gggctcatct gctcctcctg tcgttgtttc tgccggttct
     1381 ttctctcact ctttgtcaga tcagggccgg ccgggcggcg gcacgtagcc tgaaagggct
     1441 tcgatccgct ccatccacgc ccgcacccca gggaaccctt cgaggtcgta gccgccctcg
     1501 tggcagacgc gcgggtacac gtagagcgcg acgtcggcgg tagtcggaac gtccccgacg
     1561 aagaactcgc ggtccgcgag gtgccgctcc atcaccccga gcgcccggta gccctgctcg
     1621 aagaactcct ccagttgggc ctctctcgcc tccgtcatct ctacctccca catgcgccag
     1681 aggcggggac gtgagaggta gagcaggagg ctgtactgct cgaagaacat ccagcgcatc
     1741 acctgcgccc gctcaagccg gtcgtcgggc aggtacggcg ttccctcggc gaagtagagc
     1801 atgatcgcgc cggattcagg gagcattgtc ccgtcgtcca actccagcgc cggcaccttg
     1861 ccgtccgggt tgacgttctt caggaactcc ggggtgtgcg tctctccgcc cttggtgttg
     1921 tagtccacca cctcgcattc ctcgcccagc aaccccagga gcagccgtac cttgtatccg
     1981 ttccccgaca tcggggcgga gtgcaaacgc atcgagtccc acctctctcc gtagcgcaca
     2041 gtctaagtct tcttcatttt gccccgaccg ccgatcaccc gcaacgcaaa gggagcgatg
     2101 taaccccagc gcaacctgtc ctggatcttc ggcgagcgcc ggttatcgcc gcgccgtccg
     2161 gcgccatcgc gcgcggccct ccgtgtaaag gtcgccgccg tgcaggtcat tgacgacgat
     2221 aaccgggaag tcctcgacca cgagcctgcg tatcgcctcg gccccgaggt cctcgtacgc
     2281 gacgacctcc gcctccttca cgcagtcggc gagcagggcg gccgtcccct cgatagcccc
     2341 gaagtagacg cagccatgct ctctcataga ttcgcggacc gcctgggagc gcaccccctt
     2401 gccgaccatg cccttcaggc cgcgttcgat caggagcggc gagtaagggt ccatgcggga
     2461 cgcggtggtc ggaccggcgg ggccgatcgg atgtcccgga cgcgctggcg ccgggccggt
     2521 gaagtacacg atctgcccgt ccgggtcgaa cgggagctcc tctcctctct ctatggcctc
     2581 ctttatccgg gcgtgcgccg cgtcgcgggc cgtgtacaaa acgcccccga gcagcaccac
     2641 gtcgccggtc ttgagcgact cgttgccttc tctcgtcagc ggagcttcaa gacgtatctc
     2701 cccggccatc cgctacagct ccctggaggc ggtgcggtgg gagtggcagt ccaggttcac
     2761 ggcaaccggg aaggccgcga tgtgcgtcgg gaagctctcg atgtggacgc acagggcggt
     2821 ctgagtgccg ccgtatccag cggggccgat gccggtcgcg ttgatctcgt ccagtatctc
     2881 ccgctcaagc tgcgccagct tcgggtccgg gctcggctcc ccggagggtc gggtcagggc
     2941 cttcttcgcc agcatcgccg ccttctcgaa cggtcccccg atgccgaccc cgacggtaac
     3001 gggcgggctc gcgttcggac cggccttctc gatggtctcg acgatgaagt ccttcatcgc
     3061 ctcgacgccc tccgcggggg tgagcatctt cagcgcgctc atgttgtcgc agcccgcgcc
     3121 ctttacgagc atcgtcagct tgaacccgtc gccggggacc gggtcgtagt agacgatggc
     3181 cggtgtgttg tcgccggtgt tgacccggtc aaccgggctg cggacgatgg acttccgcag
     3241 atatccctcc tcgtagccgc gccggacgcc ctcgtctatg gcggcggaga tctcccctcc
     3301 cgcgatgcgg acctcactcc ccatctcgac gaagaagacc gcgtatccgg tgtcctggca
     3361 gaaggcgacg cagcgctcgc gggcgacctc ggcgttctgc agcagcctct ccaggacctc
     3421 ccggccgtgc ggcgactcct catccccgag ggcgcgccgc atcgccgcca ggtggctctg
     3481 cggcaactcc gtattggcct cgatgcacag gtcccggaca gtctcgacta gtgtttcggc
     3541 gcttatctct cgcaagtctc ctcctcatcc ctagctgata cttactatat tccccttccg
     3601 ccgtccacgg gaaggaccgt gcccgttatg aactccgagt cgtcggaggc caggtaaaga
     3661 gccgccgcgg ccacgtcctc cggccgggcg agccttccca gggggatgga ggagatgaac
     3721 gccgcccgtc cctcttccgg gtcccggtcc ccgataaacg aaccgagcat cggcgtgtcg
     3781 gtggcgaccg gggccaggca gttgacccgg atgccgtcct cggccaactc cagggcgagc
     3841 gtccgcgcga ggtgaatggc cgccgccttg gacgccgcgt aggccgtgaa gccgggccgg
     3901 gtccgtatcc ccatgacgga cgcggtgacg atgatcgatc ctccaccctc cctgagcgcc
     3961 ggtatcgccg cctgggagca caggaaaact cccttgacgt ttaccgccag cgcccggtcg
     4021 aagtcctcct ccgtggtctc ctcgccagga cggggcttgt tggtgaaacc cgcgttgttg
     4081 aacaggatgt cgaggccgcc gtaatgctcg accgccgccc ggaccatcgc ccgggcgtcc
     4141 tccgcccgcg tcacgtcgca gcggacatgt accgcctcgc cgcctcgcga cgagattccc
     4201 cgcgcaacct cctccgccct gtcagcgtcc aggtcggcta ccaccacccg cgccccctcg
     4261 gccgcgaacc tctccacgga ggcgcgcccg atccccgacg ccgcccctgt tactatggcg
     4321 actttccctt ccagtctccc atcacgaact gtcaccctcg cccctttcac tctcacgtac
     4381 ggccaggtcc aatacggtac cgccggcggc acctggcatc aatttaaacc tatccagcac
     4441 cctccggcat ctattcggct tatacaacag ctccaatcag ccgctcgcgc tacgagacgc
     4501 cgaagatcag tagcaggacc gtcagggaga tcaggcttag aaacgtagag acggccacga
     4561 cgccgctcac gagccggggc cgcacgtcga actcgatggc gagtatcatc atgttcaccg
     4621 cggtcggcat gctcgccagt atcacggcca gcttcgtcga gagcccgtcc atctgaagga
     4681 ggtaggcgag agccagcgcg agagggatgg agacgacgag cctcatgacc gtcgccagcg
     4741 cgatctcccg cctctgggtc gtcttgtttg actcggcgag ctgcaacccc aggacgatca
     4801 ggagcaccgg cacggttgcg tcgcccatca gcgcaacgcc ccggaacagg tcgttcagga
     4861 tgccgccgga ggaggcgtct aactgtatcc ccgtccaacg caccgcgagt gccgccagca
     4921 ccgcgtagac ctggggcatc cgaaagaggc gcttgaaccc gtcgagccag tctccgtgtc
     4981 ccgccccggc aatgtatacc gccacagtct gcgccaggac ggcctgaacg acgaagaaga
     5041 gcaccgcgcg ccgcagtccc tcctctccga aggcgaactg ggccagggga agcccgtagt
     5101 tgcccgcgtt catgaacatc acgcacaaca gcagcgcgga agtggtcatg gcatcgagcc
     5161 gcagcgtccg gctcatcgtc caggtcagga cgcccatcac caggacgaac aggaccatga
     5221 aggcggttat ccgcagcgtc tcgtcggccg cgaggcggac ccgcgtcagg gaggtaaaga
     5281 tcagggccgg actcagcagg tagatgctga cccggtttat ggactgcacg tccaggtcga
     5341 acctgcgccg gagcaggtaa ccgaggccca cgacgaccag gataggcaac agcacctttg
     5401 cgaagacggc aagcaccata gtagccctat atcctacaga tgtcgcgccg ctctacccac
     5461 cgccctgcgg acctattgcc ggcgggccag gtagacgccg atgagaagga cggctccgcc
     5521 gataatcttg ttgatgccaa aggtatcgct aaagaacacg atctccgaga ccacgccgac
     5581 caccgtgatg aggtactggt agacgagtac ccggttggcc cagatacggc tcttccctct
     5641 gccaacccgc gaaggggaac gccgcggcga aggcggccga gtaggctacg gccagcacgg
     5701 ttccgtcccg acgccgtccc gcccgaggcc gagcaggtac ggtgaagaga ggagtagcag
     5761 gaacggcgcg ctgaacagaa ccgggtacgt cgccaccgcc gcgctcttcc cctggtgcgc
     5821 ggacagattc ttcgatccgc gcccgcccga cgcacggagg accgtaaggc ttgagtatcc
     5881 atacggcgtg tagaactgga gcgactcggt gcccgagctg gtgcgcggtc cttacgtccg
     5941 cgtagatgag gtctgctcct taggcaaacg cgcggaggat ttcgtcgtcg tgcccttccc
     6001 catccaggag atgcagctcc tccgtcactg tacgcccgcc ggcgccgtcc acttcgtgcg
     6061 cgcctactcg gcctgtgagt gggagaaggt ccgccgaatc agggcgcacg agatcgcggt
     6121 cgaagtcctc gacccgggca gcgccaagca ggtctcggga accgggatac gggaggcgct
     6181 ccgcgatgga tcccctctgg agcatctcgt gcccaccggg tccgtgggcg cgaaatagtg
     6241 ggcgcttatg gaatctctcg attagccggc gaagatgtcc gcctaccggc tctctaactg
     6301 acagagagaa tcggctgcga tccgggccag caactcgacg ccgatttgca gggcggattc
     6361 ttctatgtcg aaacgcgagt cgtgcagacg cttcttctcc cggtcatcgg atctggccgt
     6421 accgagccgg aaataggcgc cggggtactt cagcaagaag cgtccgaagt cctctcctcc
     6481 cagggacggt tcgatctccg gcgtcgcatc gtcccccaac agatctcgcg ccgtctcggc
     6541 cacgcgctcc gcccataccg ggtcgtttat cgcggccgga tagccgtccc ggtactggat
     6601 gcgggccgta gctcccatcg cctcggtgac tccggtaact atggagtgga agcggtcctt
     6661 gaccgtctcc ttggtctccg gccggaaggt acggacggtc ccttcgagcg tcaccctatc
     6721 cgctatggcg ttgtagcggg tgccgccccg gatcttcccg attgtcaaaa ccgccgattc
     6781 cagcggattt acctcccggc tgacgacggt ctggagcgcg tttatcacct ggttggcgac
     6841 cacgatggcg tccgtggtct ggtgcggttt gctcgcgtgc ccccccacac cctctatcgt
     6901 caccttgaag cggtccgagg ctccggtcat cgcaccccgg cggacgccga tccggccaac
     6961 cggcagctcc ggccacacgt gctgggcgaa gatgacgtcg ggccggtgct cccgaaatac
     7021 tccgtccttc atcatcgggc gcgccccgcc cgtgggcgac gcctcctcgg cgggctggaa
     7081 gacgagcagc acccgccccg gcagctcttc tttcgactcg tttaacaacc acgccgcacc
     7141 cacgagcatc gccgtatgag cgtcgtgccc gcaggcgtgc atcacccctt cattcttcga
     7201 gacgaagtcg tggccgttct cttcgtgtat gggcagcgcg tcgatgtcgg cgcgcaacgc
     7261 caccgttcct ccgggcttgc ctccctcgat caagcccagc acccccgtcc cggcatagcc
     7321 cgtggtgaaa gggatgccgc gctcctccag cttctgctgt accgtgcgcg aggtggcgcg
     7381 ctcctccccg ctcagttccg ggtgctggtg gagatgtcgc cttagagaga cgatctcctc
     7441 catgtacgga gcgctctttt ccaaggccgc cctcacgcct tcagatgtcc gcgacgcgga
     7501 gcaagctcgc ttcttcgatc ctccgggcgt cgccaacccg gcgaaaagcc ggaaacggga
     7561 agagctaccg caggatctgt caacaacagc cggccaactc tccccgtgat ctccgctacc
     7621 tctccgcctc gcccgagacc cgctgcgggg actcgggctg ctcgtcctcg cggctctcgt
     7681 cgcgcagctt ccgcaggaca ccgcccgcga gcaagatgtc gcgctcgcgc ctggagaact
     7741 cggcgagcag cgtaacctcg ccaccctcgc ccttcagcgg cacctccgtg tcgccgctct
     7801 ccaggtgcga ccggatctcc ggtaactccc acttctggcc ttgctcaaac ttatcgtagt
     7861 cgtcctcgtc cttgaagcgc aaagccagga tgccctggga gatcaagttc cgccggtgta
     7921 tccgcgcgaa gctcttggct ataaccgccc ggatgccgag gtgcagcgga gcgagcgcgg
     7981 cgtgctcccg gctcgaaccc tgcccgtagt tgtgcccgcc gacgatgaac cccggcgcca
     8041 tctcctgcgc cttcttgtgg tagtcggggt cgaagcggcg gaaggtgaac ctggctatct
     8101 ccggcacgtt cgagcggaac gacatcacct cgaccccatc cggcgcaagg tcgccggtcg
     8161 atatgtcgtc ctcgaccacg atggtgacgg cgcattcgag cgagtcggga agttccggag
     8221 cctcgggcgg cggcttgatg ttcggtccgc gcggtatctc gatgccggaa gcttcctcct
     8281 ctggagcggg agccagtatc tgccggtcgt ccaccgcggg attcgccggg gcctccggca
     8341 actccggata gtcgccgagc tcacgcgggt cggtgatgac gccgcgcagg gccgtagcca
     8401 ccgccgtcgt cgggctcgac aggtagaccc ggtccgtcat cgtcccggag cgtccgggga
     8461 agttacggtt gaacgtcctg acgctcacgg aatccgaggg cggagcctgc cccatcccga
     8521 cgcacggtcc gcacgcgggc tccaacatgc gcgcccccgc gcgcacgagg tccgagtaga
     8581 cacccgtctg ggcgatggag tccaggatct gacggctccc cggcgtgacc gtaagcacga
     8641 gccccggata caccgtctcg tccctcaaga ccgcagccgc tatcgccagg tcctcgaagc
     8701 cggagttgac ggagcttccc aggcagacct gcgccgcctt ggtgcccgcg acctccgaga
     8761 cagggacgac gttaccgggc gagtgcggct gcgcgatcag gggctccagc ttgtcgagct
     8821 cgatgtgctc gtactcgtcg tagtccgctc cctcgtcggc ggccagctcc cgaaagtcgt
     8881 ctgtacgctg ctgggcttcg agccactcgc gggtgcgctc gtcggatggg aagacgccgg
     8941 tcgtggcgcc gagctcggcg atcatgttgc agatggtggt gcggcccgtc acgtctatgc
     9001 tctctacgcc ctctccgtag aactcgaaga tacgcccgag accgccgcgc acgccgcgcc
     9061 gcctgagcag ttccaggatc acgtccttcg gcgtcaccca gtccttgagc ttcccggtca
     9121 actcgacccc gacgaccttc gggacctcta tctcgaaggg ctgcccggcc atgacgagcg
     9181 ccacgtcgag cccgccggcc ccgatggcga tggagccgag agcgcccgag gaggtggtgt
     9241 gagagtcagc cccgacgagg gtcgctccgg gccgggcgaa ccgctcgacg tgcaggtagt
     9301 ggcagatgcc gttacccgga cgggagtaat agacgccgta cttcgcggcg aacgcctgca
     9361 ggaagcgatg gtcgtccggg ttcttgaagt ctagctggat gatattgtgg tccacgtact
     9421 ggacggcgag gtccacccgc gccctgtcca cacccatccg ctcgaactgg agcgaggcca
     9481 tcgtgccggt cgcgtcctga agcagtgtct gatctatccg caggcccacc tcctcgccgg
     9541 gctcgtaatc gccgtggacc atgtgctcct tgaatatctt cgtgaccaga ttctcggcca
     9601 tcgcgctacc ccttgctggt ttccctacca cctgccttca tgctacacca tcactcccaa
     9661 gggagccccc tacttcggcc gcctctgaga gaattctcca ctctattctc gctctcttga
     9721 gggcctctgg atctcgatgc gccatgacgg ttcggacagc ggtgcggagg gcccggatct
     9781 aaccggactg ataacccgta agcgcttcgg tcagcgtcgc ccacactctc tcgggctccc
     9841 gcgtgcccag atgggtcatg tgaccgcaag cctcgtatcc ccacgtccac aggtcctcta
     9901 ccccggcgga ccgggcggcc tgaacggcgg cgtggagatc gtccccatcc tccggaccga
     9961 gaccgaagcc ctggatccag acctgggcgc tgacaccgtg ctccgctccc aggtctcgca
    10021 cccggctcgc gaagccgccg acgaagtcgc gggcgggatg tccaaaggtc ttccagtaag
    10081 gatccgtcgc cagcgtgtcg aggccgggca tcctcgccac ttccgaccag tccgagaggc
    10141 cgagcggccc ctccgtcagc ggcagcagac acaccgtgct gcgcgagccc cgcgtctcga
    10201 cgtgggccac gagctcccgg acaaagccga cgagcgaggc ttcgcggaaa gcggccacct
    10261 caggcgtcat ctcgtggggc atgcgctccc catactgctc gaagaaggct ccccggcacc
    10321 gctcgcagcg gcaagcccac cgatcggtcg aaacaccaaa cctggaaggg tccgcccagt
    10381 gcggctcatc ccagaagacc ctgtcggctc cggtctcgat cgccgcgtcg gcccaggaac
    10441 gtacgaaggc gcggaacgcg gggtggtttg gacacgccgc gcctacagga gtcacatcat
    10501 caaagatctg cccgacctcc ggatgcgacg cggtaaacag gctctcggac tcgcccccga
    10561 aagcatgacc gaccgcccat gggttgatct ggacctcaag ccccgccgcg tgcgagacct
    10621 ccacgatgcg gcgcatctgg tcccggtagt agtacaggtc gttctcggag aaagtatgca
    10681 ggacgccggt aaaaccgcgt tccgccaggt catccatatc agcggcgacg tgcctgagaa
    10741 tacggttgcc gaaatatgag acccctatag agatagtcct tacccctccc tggaacttcc
    10801 gctgctattc aacatacatt ctacctgtgc cccaagccaa agacgtgtct tcaggcaagc
    10861 aactgccgga aatcccctca acactctaga aacctggagc gctggtgtca ggtctgacgt
    10921 cttccacaca ctagacgctc agaatgtcgc taatcgcttc tacaagctgc tacttgagca
    10981 cgtcgttccc ggctatccgc tttcacgatc tgcggcatag ctgtctatcc ctactcgccc
    11041 aacgcggcga gccgataagg gactcgcagg cgctcgccgg gcacgcgacg gacgcgttca
    11101 cgctccagcg ctacacccac cattacgacg ccagcgcacg ccgcaccgcc gacgccatgg
    11161 aagacatcct ccccgacgga ataccggacg gaccctaacc ggctcccggt ggtatcagcg
    11221 tggtatcaaa aagcccccgg atcatcgccg ggggctttcg tcattccgag aattccgctt
    11281 agataagccg aatcgagaga gccgacgagc ggactcgaac cgctgacctg ctcattacga
    11341 gtgagctgct ctacctgctg agctacgtcg gcctgtttcg ggcgcggatt atatcgcagg
    11401 ctccaggcgc tttcactaac tttctggttg accgtggccg ctggcgggcg tagcctgtaa
    11461 ctcctgttgt ttggaggcgt gggacctccg ggagaggagg agtatgcaga gggttatggg
    11521 cgtgtctcgt gaggcggtga tccaggctct ggacaactgc tacgacccgt gttgcaggga
    11581 gcgtaggatc agcgtggtgg atattggtct gatcgagtca atcgagatac aggaccgttc
    11641 tgtgaagatt gagatggtcc ttacgaccgg ctggtgcccg ttcgcctccc ggctgctgga
    11701 gatggtcgag gaagaggtgg gcggactgcc ggaggtggat aacgtagagg tcgaggtagt
    11761 gtgggacccg acgtggacgc cggagcggat gagccaggga gcgcgcgaga agctgcggtt
    11821 gccgcttgaa cagctcgcgc cgttgcgcga ggcgcgtttg cgcgaggcgc ggattagagg
    11881 agagagagca tgataaacga cgacgtgctg gtcttcgact gtgtggccca cccgttcaac
    11941 ttcgacccct ccaacgtcct agggaacgcg ggagagctgt tccgccaaca cctgttcgcc
    12001 ttccacaacg tgctgacgcc agagagcgag ccgaagctct cgtcagacga gttcttgaag
    12061 gagtggagca cggcggagat ctcgcggatg gtcttcgacg agtcggatac ggacatgctg
    12121 gtcgccatgc cgctgcccct tacggacctc ttccacgacg gtctctcgcc ctgggagcgg
    12181 tgcgcagagc tgcgcgaccg gaacccggag aggacggtct tctgggggac ggtcaacccg
    12241 ctggagggca aacgcgcgct ggaccagatg gaggttcagg tcaaggagta cggggcgaag
    12301 gcgttcaagt tctacaatgt acgctacgac tacggcgagc ccttcccctg gcggatggac
    12361 gacccgaggg tcgcgttccc catctacgag aaggcacagg agttggggat aaacctcatc
    12421 ggagtccaca agggtgtccc gctgggaccg cagcctatag aggcgaccca gacgtgggac
    12481 atggacggcg cggcggccaa cttcccggac ataaacttcg tcatcttcca cgtcgggctg
    12541 ccgtttatcg acgagacctg ctggcagctc gtcaggttcc cgaacctcta cgcctccatc
    12601 gcggcgacca tcaacttcgt ggtgcgttca ccgaggcagt tcgccgagtg gatcggcaag
    12661 ctaatgttct ggtgcgggga ggacaagatc atctacggtt ccgagacccc gatctggcac
    12721 ccgaagtggg cgctcgatgc tttctgggac ttcgagatcc cccaggacct cgtcgagggc
    12781 tacggttacc cgcaactaac cgaacaggcg aagcgcaaga tcctgggcga aaatctcctg
    12841 cgcctctccg gtatggatgt cgaggagaca aagcagaggc tcaacggagc cgcgtaggat
    12901 tgaggcgcgt cccgatctgg ccgggacgcg ccattacttc tactggataa tgtcccccac
    12961 cttcatcgtc ccgcgctcta taagctctat gcgatagccg tccgggtcca ccacgaacgc
    13021 gatacggtag tcgtgcccct cgacggtcat cgtcttgggc gcgagtgaga cctctacccc
    13081 gtcctgttcg agcttcgcca cggtgccttc caggtcgtcc accgtgaagg cgacgtgcga
    13141 gtagccgttc cccacgtcgt acggctcggt ctggtcgtgg ttgtgggtga gctccagcat
    13201 cggctgcgag gcgtcgggct ccatagcgaa gaagtagttg gccgccgtgt cgaagtgcat
    13261 ctcccccacg agcttcatgc ctagcttgcc gacgtagaag tccttgctcc tctccgggtc
    13321 caggatacgg tagcaggtat gtatgtatct ggtggtcatc ttgtagctcc tctcagtatc
    13381 ttgcagctaa taaacaaacg aggtctgtta tgccctccgc tcctccgttc tacacggcca
    13441 gagcttccgg tagagtccgg caaagactgc cccttctcgt aacgatctcg gactgcctgg
    13501 tgggtaacgg ccggaagctc agcctcgcca gccatgagca tccggcgctg gcagtcaaag
    13561 gcacccgcgg cgagcatggc gtcccatcac tattatcttt tatcttgcat cttcgcttcc
    13621 ctttcttcag gttacgctgc agtaccaggt acgcccagac gtctctctaa ccctctccct
    13681 cgcgcaacaa agaggaagtc tcttgagcag cccactggac cagttcgatc atgcgctccg
    13741 cctcgctcgc gtagcgccgg gcggaggtag acacgccgac cgcgccctcg atctctccgc
    13801 tgctcccgag aatgggcgcg gctacgcaac acaggttcgc ctcgaactcc tcgacatccg
    13861 tggccacgcc ctccgctcgg actctatcca gatggacctt gaacaggccc ggctggacta
    13921 tggtgcgtgt cgtgaactgc tccagcccga agccctccgc atagtcctct acaccctcgg
    13981 cgcctgtgcc ggctataaga accttgccca gagccagggc gtgtgaagct ccgtggaacc
    14041 cgcgcaccag gcccacgggc gggctcttgc atggggactt gacctcggac accgttacgg
    14101 ctccgtcaga caaaactccc agataagcgt gcctgccgga acgctgcgcc aactcctcca
    14161 gcacgggttc cgcggcagac ccgaggtccg ttccggagcg ccgctcgtgc agggtggaga
    14221 tagcagcgcc aatcctgtat cccttacgcg taggatcctt ctccaggtat ccctcttcga
    14281 tgagaatgtt gaccaggtaa tagcaagtgg agaggctcac cccgaggtct ctggccaaca
    14341 acttcgccgt cagtccctcc cgccggctga cgagatcaag aattctgaat acccgccgga
    14401 tgctctcaaa cttcgcgcca ccgcggccgc cgtccctgtc cagcgggggg acgtcgagca
    14461 tgctcaacct gcgttcaagc ctcactccgc gatcttctcc catcccattc gatcatccca
    14521 gtctccgcgt gggagacttg ctatcttgtg tccgaattta ctccgagcct gccccagacg
    14581 caaccgaagc cgggattttt cctacatcgt tggaaaatga gtagcttgtg ccttgcttgt
    14641 ggacacgcgc aggattattc tccattggca agttgcacga gggacagggg tgtccactcg
    14701 cagaaccgca cggccaatag agcacgatag gaggtggcta cggcggaaca gctttgagtg
    14761 aataggtcat gaatcaaaca catacccatg tgtcttcgta gggaaaggaa gataccttgt
    14821 acgagaaaga cggtgaaaag tacttcgtgg tggatgagca cctccacttc tgggacgcga
    14881 gcccggagaa caccacgaac aagtacggcg aagggttcat caagtgtttc tatgattacc
    14941 acgcgaacct gagccccgat gagtacacct ggaccttcga gaagtaccgc aagtactccg
    15001 aagaggacat gatgcacgac ctcttcgagg tgggatacgt ggacaaggcg atcctccagt
    15061 ccacctatct cacggatttc tacgat
//
LOCUS       DASSMB010000211         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_816038, whole genome shotgun sequence.
ACCESSION   DASSMB010000211 DASSMB010000000
VERSION     DASSMB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816038"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1333
                     /gene="eno"
                     /locus_tag="VFJ72_12610"
     CDS             38..1333
                     /gene="eno"
                     /locus_tag="VFJ72_12610"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7480348.1"
                     /translation="MTEIVAVHGREILDSRGNPTVEVEIATASGFVGRASVPSGASTG
                     ANEAVELRDGDKGRYGGKGVRTAAENVDGELAQVVLGLDATDQRTLDLAMIEADGTDN
                     KGRLGANAILGVSLAAAKASAEAAALPLYRYLGGAGAHTIPVPCANILNGGAHAANNV
                     DFQEFMVVPAGFDSYAEGLRAVAEVYQGLKKLLGERGLSGGIGDEGGFAPDLEDNGAA
                     LGLMSEAIERSGYSLGDQMSFALDPAASEFYEDGTYNLAGEGKKLSREEMVDYYARLC
                     DEYPIISIEDGLAEDDWEGWAMITERLGGRVQLVGDDLFVTNKKILAEGIEKGVGNSI
                     LIKVNQIGTLTETFETMDLAHKSGYTTMISHRSGETEDSTISDLAVAVNAGQIKTGAP
                     ARGERVAKYNQLLRIEEALGDSAFYPGRGAFNPKIRRGA"
     gene            1337..2755
                     /gene="pyk"
                     /locus_tag="VFJ72_12615"
     CDS             1337..2755
                     /gene="pyk"
                     /locus_tag="VFJ72_12615"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET7480349.1"
                     /translation="MRRTKIVATLGPATSSEEPIAALVRAGVDVTRFNFSHGDHAVHL
                     RNARTVRETAREMGRSVAVLQDIQGPKIRTGNVQDGTELVSGSRVTISQGDFLGDASR
                     LATSYENLAKDVEVGHRLLIDDGLIGLKVERIEGDDVVCEVIEGGPVSSHKGLNFPDS
                     QLSIHGLTPKDVEDLRFGMEEIHPDWVAVSFVRTADEVREVKERVREFGGNTPVISKI
                     EKHEAIENIEEIIQASDGIMVARGDLAVELSAERVPIEQKRMVARCRRLGKPVIVATQ
                     MLDSMIRNPRPTRAEVSDVANAIFDRADAVMLSGETAVGRYWLQSVQQMDRICCAAEG
                     AINYGRDIPSSASWGRGDRYDAVTHAACELAEDLEAEAILTSTQTGLSAIRVARFRPP
                     NKILAVSPIEATVRMLALVWGVTSIEGEQAGTLEDRFRGSIEAAKRAGHLQDGDEVIL
                     TGGVAGSMPGSTNALQVHTVGQ"
     gene            2769..3050
                     /locus_tag="VFJ72_12620"
     CDS             2769..3050
                     /locus_tag="VFJ72_12620"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET7480350.1"
                     /translation="MNLTRRSLRPLWVMLYAAFVGLLLASYVQPLKEIVQNRSDIPAL
                     EAKLHKTGERNTEQQRLIKELQTPAGIERAARERYGMIRDGEKVYIVEK"
     gene            3053..3499
                     /locus_tag="VFJ72_12625"
     CDS             3053..3499
                     /locus_tag="VFJ72_12625"
                     /inference="COORDINATES: protein motif:HMM:NF016314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HET7480351.1"
                     /translation="MAADPGDRAAVARQLGREPRPFRVAARCPFGMPSVIENERHRNM
                     PTSFWITCKTLDAAIARIESTGGVREAQEHLGEEKIEEIHTAHKTAYGTRVAGVREGG
                     YVKCLHAFTALHLAGAIPNPVAEWTLQRLQNPYPQDRCCTAEEPEN"
     gene            3523..3596
                     /locus_tag="VFJ72_12630"
     tRNA            3523..3596
                     /locus_tag="VFJ72_12630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3557..3559,aa:Leu,seq:taa)
BASE COUNT          708 a         1165 c         1259 g          553 t
ORIGIN      
        1 taaatacgtc tgctagtggt aagggaggat aactgctatg acggagatcg tcgctgttca
       61 cgggcgcgag atactggatt cacgcggcaa ccccacggtg gaggtcgaga tcgccacggc
      121 gagcgggttc gtcggacggg cttccgtacc ttccggggcc tccaccggag caaacgaggc
      181 cgtggagctt cgggacgggg acaaggggcg ctacggcggc aagggcgtcc gaaccgctgc
      241 agagaacgtg gacggtgagc tggcgcaggt agtcctcggg ctcgacgcca ccgaccagcg
      301 cacgttggac ctggcaatga tcgaagccga cggcacggac aacaaaggcc gcctcggcgc
      361 gaacgccatc ctcggggtct cgctggcggc ggcgaaggct tccgctgagg cggcggcgct
      421 gccgctatat cgctatctgg gcggggccgg ggcgcacacc atcccggtgc cgtgcgcgaa
      481 catcctcaac ggcggcgctc acgccgcgaa caacgtggac ttccaggagt tcatggtggt
      541 gccggctggc ttcgatagct atgccgaggg tctgcgggcc gtggcggagg tctaccaggg
      601 cctcaagaag ctgctcggcg agcggggtct ctccggcggc atcggggacg agggcgggtt
      661 cgccccggac ctggaggaca acggggcggc gctcgggctc atgtccgagg ctatagagcg
      721 gagcgggtac tccctggggg atcagatgtc cttcgcgctc gacccggcgg cctccgagtt
      781 ctacgaggac ggcacgtata acctggccgg agaagggaag aagctctccc gcgaggagat
      841 ggtggactac tacgccaggc tgtgcgacga gtacccgatc atcagcatcg aggacgggct
      901 cgcggaggac gactgggaag gatgggccat gatcacggag cggctcggcg gccgggtcca
      961 gctcgtcggc gacgacctgt tcgtgaccaa caagaagatc ctcgccgaag gcatcgagaa
     1021 aggggttggc aactccatcc tgatcaaggt caaccagatc ggcactctca cggagacctt
     1081 cgagacgatg gacctggcgc acaagtcggg atacacgacc atgatcagcc accgcagcgg
     1141 cgagacggag gactctacga tatccgacct cgccgtcgcc gtgaacgcgg gacagataaa
     1201 gaccggcgcc ccggcccgcg gcgagcgcgt ggcgaagtac aaccagctcc tacgtatcga
     1261 agaggcgctg ggcgactcgg cgttctaccc cggacgcggg gctttcaacc cgaagatccg
     1321 gcgcggagcc tagcggatgc gccggaccaa gatagtcgcc actctcggcc cggcgacctc
     1381 ctcggaggag ccgatagcgg cgctcgtgcg cgccggggtg gacgttaccc ggttcaactt
     1441 ttctcacgga gatcacgcgg tgcatctgcg gaacgcccgc accgtgcggg agacggcgcg
     1501 ggagatggga cgcagcgtcg ccgtgctgca ggacatccag ggtccgaaga taaggaccgg
     1561 caacgttcag gacggtacgg agctggtctc gggtagccgc gtgaccatct cgcagggaga
     1621 cttcctgggg gacgcgagcc ggcttgcgac ctcctacgag aacctcgcta aggacgtcga
     1681 ggtcggccac cggctgctca tagatgatgg cctgatcggc ctgaaggtcg agcgcatcga
     1741 gggcgatgac gtggtgtgcg aggtaataga gggcggcccc gtctcctcgc acaagggcct
     1801 gaacttcccg gattcccagc tctccatcca cgggctgacg ccgaaggacg tggaggacct
     1861 gcggttcggg atggaggaga tccacccgga ctgggtcgcg gtctcgttcg tgcgcaccgc
     1921 cgatgaggtg cgcgaggtga aggagagggt cagggagttc ggtggcaata cgcccgtcat
     1981 ctccaagatc gagaagcacg aggcgataga gaacatcgag gagatcatcc aggcctccga
     2041 cggcattatg gtggcgcggg gcgacctggc ggtggagctt tccgccgagc gcgtcccgat
     2101 agagcagaag cgcatggtcg cccgctgccg caggctcggc aagcccgtca tcgtcgccac
     2161 ccagatgctc gactccatga tccggaaccc ccgtccgacc cgcgccgagg tctcggacgt
     2221 ggcgaatgcg atcttcgacc gggcggacgc ggtgatgctc tccggcgaga cggcggtagg
     2281 gcgctactgg ctgcagagcg tccagcagat ggaccgcatc tgctgcgcgg cggagggcgc
     2341 tatcaactac ggtcgggata taccgtcgtc cgcctcctgg ggccgcggcg accgctacga
     2401 cgccgtgacc cacgccgcct gcgagctggc ggaagacctg gaggccgagg cgatcctgac
     2461 ctccacccag acgggccttt cggctatcag ggtggcgagg ttccgcccgc cgaacaagat
     2521 cctcgcggtc agccctatcg aggcgacggt gcggatgctg gcgctggtct ggggagtaac
     2581 gtctatagag ggcgagcagg ccgggacgct ggaggaccgc ttccggggct ctatcgaggc
     2641 ggccaaacgg gccggacacc tgcaggatgg agacgaggta atcctgaccg gcggtgtcgc
     2701 gggctccatg ccgggttcca ccaacgctct acaggtccac accgtcgggc agtagggttg
     2761 atcggacgtt gaacctcacc cggcggagtc tgcggcccct gtgggtcatg ctctacgcag
     2821 ccttcgtcgg actccttctc gcctcttacg tgcaacctct gaaagagata gtgcagaacc
     2881 gttccgatat ccccgccctc gaagcgaagc tccacaagac cggcgagcgc aataccgagc
     2941 agcagcggct gatcaaggag ttgcagaccc cggccgggat agagcgcgcc gcccgcgagc
     3001 gctacggcat gatccgggac ggcgaaaaag tctacatagt cgagaagtaa gcatggccgc
     3061 cgaccccgga gaccgcgccg ccgtcgcccg ccagctaggc cgggagcccc ggccattccg
     3121 cgtcgccgcc cgctgtccgt tcgggatgcc gagcgtcatc gaaaacgagc ggcacaggaa
     3181 catgccgacg agcttctgga tcacctgcaa gaccctcgac gcagccatcg cccggataga
     3241 gtccaccggc ggcgtccggg aagcccagga acacctgggt gaagagaaga tagaagagat
     3301 ccacaccgcc cacaagaccg cctacggcac ccgcgtcgcg ggcgtgcggg agggcggcta
     3361 cgtaaaatgc ctccacgcct tcaccgccct ccacctcgca ggcgccatcc cgaaccccgt
     3421 tgccgaatgg accctccagc gactacaaaa cccctatccc caggaccgct gttgcacggc
     3481 cgaggagccc gaaaactagc tgctatactc ccgaatgagt gcgggggcgt agcccaatcg
     3541 gcagaggcag aggacttaaa atccttccag tgtgggttcg agtcccaccg cccctactga
     3601 gaatagttcg gtcgacagag cgtcagattg tggctccgaa ggtcgcggtt tcgagccctg
     3661 tcgggcatcc tctctgatcc cgtat
//
LOCUS       DASSMB010000212        11049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1579999, whole genome shotgun sequence.
ACCESSION   DASSMB010000212 DASSMB010000000
VERSION     DASSMB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11049
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1579999"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFJ72_12635"
     CDS             complement(<1..523)
                     /locus_tag="VFJ72_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HET7480352.1"
                     /translation="MRLNRTIVLLAAIAVTLMAYMSFGAKTADAQTVTLDPTTVDFGA
                     VVLDTTSTPQTVTITNTTAGPLTITGATLSGPNAGDFTLLNQLPVGGVTINPNGTVTF
                     DVAFSPSALGSRVANLDFTTTTVPVSSAALTGTGVAPTSVNSAPTIAKVSPAPRSKVK
                     DTTPTIKATVRDRG"
     regulatory      complement(676..753)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            964..1950
                     /gene="gmd"
                     /locus_tag="VFJ72_12640"
     CDS             964..1950
                     /gene="gmd"
                     /locus_tag="VFJ72_12640"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7480353.1"
                     /translation="MITGITGQDGAYLAQHLLERGYRVVGAFRRASTVATTRLDSLGI
                     TDEIEMVPFDLTDHGNMRRVIDAVEPDEIYNLAAQSFVGVSFEQPVTTGEITGVGVVR
                     LLEAIREANPEIRFYQASTSEMFGKAPAPQNEETLFHPRSPYAVSKLYAHWSTVNYRE
                     AYGMHASSGILFNHESPLRGAEFVTRKITLAAARIKHGLQDELKLGNLDARRDWGYAR
                     EYVEAMRLMLQQPEPGDYVIATGETHSVREFVEAAFEAAELDWEQHVVTDPAFLRPAE
                     VDLLLGDITKAKKKLGWEPKTTFKDLVALMVEADLKRAAFEARHGRESAEIR"
     gene            1951..2868
                     /locus_tag="VFJ72_12645"
     CDS             1951..2868
                     /locus_tag="VFJ72_12645"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7480354.1"
                     /translation="MPHALVTGASGFAGPFLIQHLLKEGYEVTGAVHGPDKKLPGECH
                     AVSLDVTDARAVRRTVAELSPDEVYHLAGLTRPASGAVGEFYAVNFGGALNLLEAARE
                     EAPEIGILLVGSAYAYGRVDRPIAESEPLNPTNHYGVSKAAADMLGHSYALQGLRVVG
                     VRPFNHSGPGQSPDFLLPTLVEQFAEIKAGRREPSVKLGNLDSVRDLSDVRDVVRAYV
                     LALRKGNPGEAYNVGSGRGIPVRELFEMVRRQAGVEVELIVEPSRVRAGDIPYLVADI
                     NKTGKELGWRPTMPLERTVREMLEAAGAV"
     gene            3055..4425
                     /locus_tag="VFJ72_12650"
     CDS             3055..4425
                     /locus_tag="VFJ72_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET7480355.1"
                     /translation="MLAFAVLLLMNGGTASESALAHEVSKPNIILILTDDMRADDLKF
                     TPRTQNLLGGGGVTFKNAFVTDSICCPSRSTILRGQYAHNHSILSNHYPLGSGIKFRE
                     LGRDNSTVATWLHSSGYRTGLFGTYLKFYDGSYVPPGWDDWYGWMGSNQFNENGQVIA
                     NTSDIHITDRLSNRADAFVRQGVASGEPFFAYIAPTAPHPPAIPPDRYKDAYPNLVAP
                     RDASFNERDVSDKMSWVRNHPLLTANEMSGIDSLYRNRARSLLAVDDMVERLVNTLTE
                     TGALENTYIVFTSDNGFHMGEHRLPPMKQTAYEEDIRVPLLIRGPGLPAGESRTAFAL
                     NNDFAPTFADWADASVPISVDGRSLSPILSSPLTTGWRTSFLVEKWDTNAMPQSYKAV
                     RTADWTYVRYSNGEKELYNLSKDPQELRNVAGDTDEKTLHALDYRLDTLKACKATTCR
                     SAENRP"
     gene            complement(4422..5249)
                     /gene="rfbD"
                     /locus_tag="VFJ72_12655"
     CDS             complement(4422..5249)
                     /gene="rfbD"
                     /locus_tag="VFJ72_12655"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HET7480356.1"
                     /translation="MLVTGAGGQLGRELAELLPEGGHETIAVARSDLDIADFASVERA
                     LEEYVPDIVINAAAYTNVDGCETETDLAYRVNALGPRNLAQLCERMGCTLLHVSTNYV
                     FDGQRERPYEPFDPPNPISAYGRTKLAGEEYVRDLTTCWYVVRSAGVYGRGHNFVRTM
                     LRVAGERETLKVKNDEFISPTYARDLAGGIIQIIESGRYGLYHLTNSGSCSWYEFTKE
                     IFRLTGVETEVIPIPGSEYPLPAARPANGVLSALGSPELRHWREALEDYLRREAVRR"
     gene            complement(5252..6223)
                     /locus_tag="VFJ72_12660"
     CDS             complement(5252..6223)
                     /locus_tag="VFJ72_12660"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7480357.1"
                     /translation="MRCLLVGGSGFMGTHLTARLLDDRHSVRVYSRGPGRFRPTSADA
                     EFVEGELGNAGLVREAVAGMEVVYHFVSTTLPKTSNDDPIYDVRSNLVDTLVLLEACV
                     EAGVRKVIFTSSGGTVYGPPRTVPIGEDHPTDPITSYGIVKLAIEKYLGLFRYLHGLN
                     CAVLRISNPYGPYQDPAGQQGVISVFLNRILAGEPITIWGDGTVVRDYLYVADLVDAL
                     VLAAEVETESKVFNIGSGRGAALKEIIALMAEVTGEKPMVEYLPGRTLDVPANVLDVR
                     RAREELGWTPRTGLADGMAETWAWIQTLTSDIQERPRGEGSMRRSPH"
     gene            complement(6225..7577)
                     /locus_tag="VFJ72_12665"
     CDS             complement(6225..7577)
                     /locus_tag="VFJ72_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7480358.1"
                     /translation="MRILFLAHQFFPEHYAGTEVLTLGLAKELKARGHEPQVFAAKRS
                     RPIHDMEPGEIEDYEYAGIPVRRVGRPTEGLRRPYRLDYENETMAERAREYAREIWPD
                     VVHAMHLQGLSASVVPALKGMGLPVVYTATDFWAVCPVVDLMRHDGVMCSGPDIAHCP
                     RCILSRQPATRLRKAANFTPGLALKIAGRLSETGLAERSLPLRQIRDIKRRPQSIREW
                     LKLVDRIIAPTRLTRNLLSSNGVGDGDIEVSHYGIDVSDIMEAPRNENVPPPLRVGFI
                     GTLGYHKGADILVRAFKRLPEDLDATLTIYGNLERFESFVKGLRDLIGGDPRITLAGT
                     FPPERIGHVLADMDILVVPSRWYENTPLVVYSAFAAGTPVVATNLGGLSEVVRHGENG
                     LLFGLGDDDDLSRQLRRLCEEPDLLTRLRAGIGHVKTVEENVTELEEIYASLAREGTS
                     "
     gene            complement(7594..8724)
                     /locus_tag="VFJ72_12670"
     CDS             complement(7594..8724)
                     /locus_tag="VFJ72_12670"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7480359.1"
                     /translation="MNLERIVERLRDYRLKNERFDRLVGSVLSNKQGAKAVRSAVAAA
                     RSVSRRGQTLAPGDYDTVFFVAGEMRSGTSWLRHSLSDHPEIACGQEGSFFGRGYDHE
                     EIPVYTAPVSSLTRALAVSKEFKTWHDLPWNLWSDGYEQDLKALSRLSVDYFLSKEVA
                     RTGRRIVGDKSPQHTENLDEIYEIYPDARIIHIVRDGRDVAISAMHHWWRLAQDREGG
                     LFEMTPEELKIRDEYTEDREGFVASGRSIFTGERLAQLARRWDYRTGKGRRDGTALYG
                     EKYLEIRYEDLLLDTPHLFGRVLEFLGAGSDEKVIARCLRSSDFERASNRQQGQEDSA
                     SFFRKGVAGDWRNVFTERDREIYRDIAGERLVEFSYENSLDW"
     gene            complement(8745..9725)
                     /locus_tag="VFJ72_12675"
     CDS             complement(8745..9725)
                     /locus_tag="VFJ72_12675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7480360.1"
                     /translation="MTDSSLQVSVVIPTYNAGSGFEELLRRISAQKGDFERELILIDS
                     GSTDGTVELAERYGAVVRRVPQEDFNHGATRNLGISHARGRYVALTVQDAVPLDESWL
                     AAMVENLERDERVAVVYGRHVPRPDAGPLTRALVKGLASAGLERRVQEIRDPNEYEAL
                     RPQEKRRTAAFDNVSSCLRRSVWEEFPFEKTSFAEDLRWGKRVVEAGYRIVYEPCSVV
                     VHSHERGALYDLRRYYVDQLVLIELFGARMVPNLSRLALGIPYSTRHVYRLLRCEEPA
                     PGKRLRHLLTAFAYAVPTQLGAYLGGKSEVLARQSPRLHDRLHRFLSKGV"
     gene            complement(9722..10699)
                     /locus_tag="VFJ72_12680"
     CDS             complement(9722..10699)
                     /locus_tag="VFJ72_12680"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7480361.1"
                     /translation="MARRVRTGLNRSRLWLAGKYREVPLTVASWGMKLSRSSAEARAG
                     GVSPENLVWIFGSGRSGSTWLRSMMGDMDRHRVWEEPLVGRLFGEFYERAQHSNLSRP
                     DFIMAAATRKGWTRSIRNFVLDGAGYARPLLGPDDYLVIKEPNGSTGAPLLMEALPES
                     RMILLIRDPRDVVASTLDGAKEGNWLHEWRNEGGKNLAAPDGDPETFVRVRSRAYLQH
                     AGNAKRAYDLHRGRKVFVRYEELRENTLGTMKRIYSALEIPVDEKKLARVVEKHAWEN
                     IPEKNKGEGKFYRKATPGGWREDLTPRQARLVENIAGPLLKEFYPENTS"
     gene            complement(10746..>11049)
                     /locus_tag="VFJ72_12685"
     CDS             complement(10746..>11049)
                     /locus_tag="VFJ72_12685"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HET7480362.1"
                     /translation="KRAYDAHRGYKTVVRYEDLRADTLATMKRLYAELGIPVDEEGLI
                     RAVEKHSWENIPEEQKGEGKFYRKGKAGGWREDLTHHQVNTIERIAAPLIKEFYSA"
BASE COUNT         2000 a         3549 c         3459 g         2041 t
ORIGIN      
        1 tccccctgtc acgcaccgtc gccttgatgg tcggcgtggt gtccttgacc ttcgagcgcg
       61 gggcgggact gaccttggcg atggtagggg cggagttcac cgacgttggg gccactccgg
      121 tcccggtcag ggccgcactg ctcaccggaa cggtggtggt ggtgaagtcc aggttggcga
      181 cccggctacc gagggccgag ggcgagaaag cgacatcgaa cgtgacagtg ccattagggt
      241 tgatggttac accgccgacc gggagctgat tcagcaacgt gaagtcgccg gcattaggcc
      301 cagagagagt ggcgccggtg atcgtcagag gtccggcagt ggtgttggtg atggtcacgg
      361 tctgcggggt gctggtggtg tcgaggacca cagcgccgaa gtcgaccgtg gtgggatcca
      421 gggtcaccgt ctgcgcgtcg gcggtcttgg cgccaaagct catgtacgcc atcagcgtta
      481 ccgctatggc cgcgagtagg actatagtcc tattgagccg cacggaccca ttcgctaaac
      541 tagatatgtt catagagtat ctccgtatcg tttctatgga cttggcttcg gggtgttgat
      601 ggcactccga agcccttttc aaggcttcta cacttgctca agggttcgcg atcgagttaa
      661 ccttgtggac ctccttccgg cgacccggcc acctctcgaa gaggtccgta ggttttgcgc
      721 ccccgcctca cgacgggttt gcctttttca gggacatgag tagtgtaccc ttgatctgct
      781 tgatctaaaa ggaagattgt tcacaatttt gattgattta aacaagattt cacgagtatt
      841 cacagcgtat aactgactgg cacctcaaga gccgcgattt tacggcttgc cgcgtagacg
      901 gtagactcgg gcaatgtcga agcaatagtc ggatgatgga gggccattat taataaaggc
      961 gcgttgatca cgggcatcac cggccaggac ggagcatacc tggcgcagca tctgttagag
     1021 agagggtacc gggtagtcgg ggccttcagg cgggcgagca ccgtggcgac cacccggctg
     1081 gactctctag gcatcacgga cgagatagag atggtgccat tcgacctgac agaccacggc
     1141 aacatgaggc gcgtaatcga tgccgtcgaa ccggatgaga tctacaacct cgccgcccag
     1201 agcttcgtcg gggtctcctt cgagcagccg gtgacaacgg gcgagatcac cggcgtcggc
     1261 gtcgtgcgct tgctggaagc catccgcgag gcgaacccgg agatccgctt ctaccaggca
     1321 tccacgagcg agatgttcgg caaggccccc gctcctcaga acgaggagac tctgttccat
     1381 ccccgcagcc cctacgccgt ctccaagctc tacgcccact ggagcacagt caactaccgc
     1441 gaggcctacg ggatgcacgc ctcctcgggg atactcttca atcacgagag ccccctgaga
     1501 ggcgcggagt tcgttacgcg caagatcacg ctggcggcgg cccgcatcaa acacggcctt
     1561 caggacgagt tgaagctcgg caacctggat gcccggcgcg actggggcta cgcccgcgag
     1621 tacgtcgagg cgatgcgcct catgctccag cagcccgagc cgggagacta cgtgatcgcc
     1681 accggcgaga cccactcggt ccgcgagttc gtcgaggcgg ccttcgaggc tgcggaactc
     1741 gactgggaac agcacgtcgt caccgacccc gccttcctgc gcccggcgga ggtagacctg
     1801 ctcctcggcg atatcacgaa ggcgaagaag aagctcggct gggagccgaa gactacgttc
     1861 aaggacctcg tcgccctcat ggtggaggcc gacctaaagc gggcggcctt cgaggcccgg
     1921 cacgggcgcg agagcgcgga gatccgctaa gtgccccacg ccctcgtaac cggagcctcc
     1981 ggcttcgcgg gaccgttcct gatccagcac ctcctcaaag aaggctacga ggtcaccggg
     2041 gccgtccacg gaccagataa gaaactcccc ggagagtgcc acgcggtgag cctggacgtg
     2101 accgatgcca gggccgtgcg gaggaccgtc gcggagctat cgccggacga ggtctaccat
     2161 ctggctggcc ttacccgtcc ggcgagcggc gcggtcgggg agttctacgc ggtcaacttc
     2221 ggcggcgccc ttaacctgct ggaggccgcg cgcgaagaag cgccggagat tggaattctc
     2281 ctggtcggga gcgcctacgc ttacggcagg gtggaccgcc ccatcgccga atccgagccc
     2341 ctgaacccca ccaaccacta cggcgtctcg aaagcggcgg cggacatgct gggacacagc
     2401 tacgccttgc agggactgcg ggtcgtgggc gtccgcccgt tcaaccactc cggtcccggc
     2461 cagagcccgg acttcctgct cccgactctg gtagagcagt tcgccgagat aaaggccggc
     2521 aggcgcgagc cctccgtgaa gctcggaaac ctcgactcgg tgcgcgacct ctccgacgtg
     2581 cgggacgtgg tgcgagccta cgtcctcgcg ctgcgaaaag gcaaccctgg cgaggcgtac
     2641 aacgtcggca gcgggcgtgg aataccggta cgggagcttt tcgagatggt gcgtcggcaa
     2701 gccggggtcg aggtcgagct catcgtcgag ccctcccgcg tgcgggcggg cgatatcccc
     2761 tacctcgtag ccgacataaa caagaccggg aaagagttgg gctggcgtcc gacgatgcca
     2821 ctggagcgga cggtcaggga gatgctggag gctgccggcg cggtctgata aaaggtccgt
     2881 tagagacctc gcatccgttt acgctgatgt gaacggaccg tgcagaaccc ggctcagggc
     2941 atgatgcgcc cagacgtatg atgctgtaga gtaatgtctg ttacctgtga tatttgggga
     3001 gcgatgttgg gcaaagtact gagtctttca ggatgccgta tatggtttac gaccatgctc
     3061 gcattcgccg tcttgttact catgaacggc ggcaccgcca gcgaatcggc gctggcgcac
     3121 gaggtaagca agcccaacat catcctgatc ctcaccgacg atatgagggc cgacgacctc
     3181 aagttcacgc ccaggacgca gaacctcctg ggcggcgggg gtgtcacgtt caagaatgcg
     3241 ttcgtcaccg actcgatctg ctgtcccagc aggtccacca tcttgagggg ccagtacgcc
     3301 cacaaccaca gcatcctctc gaaccactac cctctcggga gcggaataaa gttccgcgag
     3361 ctcggccggg acaattccac cgtggcgacc tggctccatt cctcgggcta tcgcaccggt
     3421 ctcttcggca cctacctcaa gttctacgac ggctcctacg tgccgccggg gtgggacgac
     3481 tggtacggat ggatgggcag caaccagttc aacgaaaacg gtcaggtgat agccaacacc
     3541 tccgacatac acattaccga ccgcctctcc aacagggccg acgccttcgt ccggcagggc
     3601 gtcgcctcgg gcgaaccgtt cttcgcctat atcgcaccca cggcgcccca tccaccagcg
     3661 atcccgcctg acaggtataa ggatgcgtat ccaaacctcg tagcgcccag ggatgcaagc
     3721 ttcaacgagc gtgacgtctc ggacaagatg tcgtgggtca ggaaccatcc gctgctcacg
     3781 gcaaacgaga tgtccgggat agattctctc taccgcaacc gcgctcgctc gctgctggcc
     3841 gtggacgata tggtagagcg gctggtcaac accctcaccg agaccggcgc cttggagaac
     3901 acttacatag tcttcacctc ggacaacggt ttccacatgg gcgagcatcg cctaccaccc
     3961 atgaagcaga ccgcctacga ggaggacata agggtaccgc ttctcatccg cggccccggt
     4021 ttgcctgccg gagagagcag gacggccttc gccctcaaca acgacttcgc ccccaccttc
     4081 gcggactggg cggatgccag cgtgcccatc tccgtggacg gccgctccct ctcgcccatt
     4141 ctctcctcac ccctcactac gggctggagg acctcgttcc tggtagagaa gtgggacacg
     4201 aacgccatgc cgcagagcta caaggccgtg aggaccgccg actggaccta cgtcagatat
     4261 tccaacggcg agaaggaact ctacaacctc tccaaagatc cccaggagct aagaaacgtc
     4321 gccggggata cggacgagaa gacgctgcat gcgctggact atcgtctcga caccctcaaa
     4381 gcctgcaagg ccaccacatg ccggtcggcc gagaaccggc cctagcgccg caccgcttcc
     4441 cgccgcaggt agtcctccag cgcctcccgc cagtggcgaa gctcggggct tccgagcgcg
     4501 gagaggacgc cgttggccgg acgagcggcg ggcagtgggt actcggagcc gggtatgggg
     4561 atgacctccg tttcgactcc ggtgaggcgg aagatctcct tcgtgaactc gtaccaggag
     4621 caggacccgg agttggtcag gtggtagagg ccgtaccgcc cagactcgat tatctggata
     4681 atccccccgg cgaggtcgcg ggcgtaggtg ggcgagatga attcatcatt cttgaccttg
     4741 agcgtctccc tctctccggc gacacgcagc atggtccgaa cgaagttgtg gccccggccg
     4801 tagactcctg cggagcggac gacgtaccag caggtcgtga ggtccctgac gtactcctcc
     4861 ccggcgagct tggtgcggcc ataggcgctg atggggttcg gcgggtcgaa gggctcgtag
     4921 ggccgctcgc gctggccgtc gaagacgtag ttggtgctga cgtgcagcag ggtgcagccc
     4981 attcgctcgc agagttgcgc caggttgcgc ggaccgaggg cgttgacgcg gtaggcgagg
     5041 tctgtctccg tctcgcagcc gtccacgttt gtgtaggcgg cagcgtttat gacaatgtcc
     5101 ggcacgtact cctccagcgc ccgctctacg gaagcaaaat ctgctatgtc gaggtcgctc
     5161 cgggcaacag ctatggtctc atggccgcct tcggggagga gctcggctag ctcccgacct
     5221 agctgacctc cggcgccggt cacgagcacc ttcaatgcgg cgacctcctc atactccctt
     5281 ctccacgcgg tctctcttga atgtctgagg tgagggtctg gatccaggcc cacgtctcgg
     5341 ccatgccgtc ggccaacccg gtccggggtg tccagcctaa ctcttctcgc gcccgtctca
     5401 cgtcgagcac gttggccggg acatccagcg tccggcccgg cagatattcg accattggct
     5461 tctctccagt gacttcggcc atgagagcta ttatctcctt cagcgcggcg ccccggccgc
     5521 tgcctatgtt aaaaactttg ctctccgtct ctacctccgc ggccagcacg agggcgtcta
     5581 caaggtccgc gacgtagagg tagtcgcgca cgacggtgcc gtcgccccag atggttatcg
     5641 gttcaccggc gaggatgcgg ttgaggaaga cggagatcac accctgctgc cccgccgggt
     5701 cctggtaggg gccgtaggga ttggagatgc gtagcacggc acagttcagg ccgtgcaggt
     5761 aacggaagag ccccagatac ttttcgatag cgagcttcac gatgccgtag gaggttatcg
     5821 ggtccgttgg atggtcctcg ccgatgggca cggtccgggg cgggccgtag acagtgccgc
     5881 cggaggatgt gaagatcacc ttgcgcaccc cggcctccac gcaggcttcg aggagcacca
     5941 gggtatctac gaggttggaa cgtacgtcgt agatagggtc gtcgttcgag gtcttgggga
     6001 gggtggtgct gacgaagtga tagacgacct ccatcccggc cacggcctcc cggacgagcc
     6061 cggcgttccc gagctccccc tcgacgaact ccgcgtccgc ggacgtcggc cggaacctac
     6121 cgggaccccg gctgtaaacc ctgacggagt gcctgtcgtc gagcagccgc gcggtgaggt
     6181 gcgtgcccat aaagccgcta ccgccgacga gcaggcacct caagctaact ggtcccctcg
     6241 cgagcgaggg aggcgtagat ctcctccagc tcggtcacgt tctcctcgac ggtctttacg
     6301 tgtccgatgc cggcacgcag cctcgtgagc aggtccggtt cctcgcagag gcgccggagc
     6361 tggcgcgaca ggtcatcgtc gtctccgagc ccgaagagca gcccgttctc tccgtgccga
     6421 acgacctcgg agagcccgcc gaggttcgtg gcgacgaccg gcgtcccggc ggcgaaggcc
     6481 gagtagacga cgagcggagt gttctcgtac cagcgagagg ggacgaccag gatgtccatg
     6541 tcggcgagga cgtgcccgat cctctccggc gggaacgtcc ccgcgagcgt gatcctcggg
     6601 tcgccgccga tgaggtcccg caatcccttc acgaacgact cgaagcgctc caggttgccg
     6661 tagatcgtga gggtcgcgtc gagatcctcc ggcagcctct tgaacgcccg caccaggata
     6721 tccgcgccct tatggtagcc tagggtgccg atgaacccga cccgcagcgg cggcggcacg
     6781 ttttcgttgc gcggggcctc catgatgtcc gagacgtcga ttccgtagtg ggagacctct
     6841 atgtcgccgt ctcctacacc gttggaggag agcaggttcc gcgtgagtcg ggtaggagcg
     6901 atgatcctgt ccaccagctt caaccactcc cggatgctct gcgggcgacg ctttatgtcc
     6961 cttatctggc gcagcggcaa cgaccgctcc gcgaggccgg tctcggagag ccggccggcg
     7021 atcttgagag caaggccggg cgtaaagttc gcagccttcc gaagacgggt ggcgggctgg
     7081 cggctcagga tgcaccgggg gcagtgggct atgtccgggc cgctgcacat gacgccgtcg
     7141 tggcgcatca gatccacgac cggacagacc gcccagaagt ccgtggccgt atagacgacc
     7201 ggcagaccca tgcccttgag cgccggcacg acgcttgccg agagcccctg gagatgcatg
     7261 gcgtggacca catcgggcca tatctcccgg gcgtactcgc gggcgcgctc cgccattgtt
     7321 tcgttctcat agtccaggcg atacgggcgg cgaagacctt ccgtcgggcg tcccacacgg
     7381 cgcacgggga tgccagcgta ctcgtagtcc tcgatctcgc cgggctccat gtcgtggatg
     7441 gggcggctgc gcttggcggc gaagacttgc ggctcatgtc cgcgggcctt caactccttc
     7501 gccagcccga gcgtcaggac ctcggtgccc gcgtagtgct cgggaaagaa ctggtgggcc
     7561 aggaagagta tcctcacggg ctgccggcca ccgctaccag tcgagggaat tctcgtagct
     7621 gaactccacg agccgctcgc cggcgatgtc gcggtagatc tccctgtccc gctccgtgaa
     7681 gacgttccgc cagtcgcccg ccacgccctt cctgaagaac gaggcggagt cctcctggcc
     7741 ctgttggcgg ttcgacgccc gctcgaagtc gctggagcga aggcagcgcg caatgacctt
     7801 ctcgtcgctg ccggccccca ggaactccag cacccggccg aagaggtgcg gcgtgtcgag
     7861 gagcaggtct tcgtaccgga tctccagata cttctcacca tagagcgcgg tcccgtcgcg
     7921 gcggcccttg ccagtacggt agtcccaccg ccgggcgagt tgcgcgagcc gctccccggt
     7981 gaagatagag cgcccggagg cgacgaaccc ttcccgatcc tcggtgtact cgtcgcggat
     8041 cttcagctcc tccggtgtca tctcgaagag gccgccttcg cggtcctgtg cgagccgcca
     8101 ccagtggtgc atcgcggaga tcgccacatc gcggccgtca cggacaatgt ggatgatcct
     8161 ggcgtcgggg tagatctcat atatctcgtc caggttctcg gtgtgctgcg ggctcttgtc
     8221 ccccacgatc ctgcgccccg tccgggcgac ttctttggag aggaagtagt ctaccgaaag
     8281 cctgctcagg gccttcaggt cctgctcgta gccgtcggac cagaggttcc acgggagatc
     8341 gtgccaggtc ttgaactcct tagagacggc gagggcacgg gtgaggctcg agacgggtgc
     8401 ggtgtagacg ggtatctcct cgtggtcgta gccgcggccg aagaagctcc cttcctggcc
     8461 gcaggcgatc tcggggtggt cgctgaggct gtgccggagc caggaggtcc cggagcgcat
     8521 ctcaccagcg acgaagaaga ccgtgtcgta gtctccgggc gcaagggtct ggcccctgcg
     8581 agagacggag cgagccgcgg cgacggcgga gcgcacggcc ttggcaccct gcttattcga
     8641 tagcacgcta ccgaccagac ggtcgaaccg ctcgttcttg agccggtaat ctcgcaagcg
     8701 ttccacgatg cgttcgaggt tcatggagcg gattatatgg acgatcagac acctttgctc
     8761 aggaaccggt gcaatctatc gtggagtctc ggactctgac gagcaagcac ctcgctcttg
     8821 cctcccaggt acgctccaag ctgggtcggg acggcgtaag cgaaagcggt caagaggtgt
     8881 cgcaacctct tcccgggcgc tggctcctcg caccgcagca gacggtaaac gtggcgcgtc
     8941 gagtatggaa tgccgagagc gagacgcgaa aggttcggca ccatccgggc gccgaacagc
     9001 tctataagga ctagctggtc cacatagtag cgcctgagat cgtagagagc gccgcgttcg
     9061 tgggagtgca caaccacgga acatggctca tagacgatcc tgtacccggc ctcgactacc
     9121 cgcttacccc agcgcaggtc ctccgcgaaa ctcgttttct cgaaagggaa ctcctcccac
     9181 accgagcgtc gcaggcagga ggacacgtta tcgaaggcag cggtacggcg cttctcctgg
     9241 ggacgcagcg cctcgtactc gtttgggtcc cggatctcct gcaccctgcg ctccagtccg
     9301 gcagaggcaa ggcctttcac cagcgcccgc gtcagcggtc cggcatccgg gcgtgggaca
     9361 tgccgtccgt agaccaccgc aactcgctca tcgcgctcca gattctcgac catcgccgcc
     9421 aaccaactct catcgagcgg cacggcgtcc tgcaccgtga gggccacgta cctgcccctt
     9481 gcgtgcgaga tgccgaggtt gcgcgtcgcg ccgtggttga aatcttcttg cggaacccgg
     9541 cgcacgacgg ctccgtagcg ttcggcaagc tcgaccgtgc cgtccgtgga accggagtcg
     9601 atcaagatca actctcgctc gaaatcgcct ttctgtgcgg aaatcctccg gagcagttcc
     9661 tcaaaaccgg agcccgcgtt gtaggtcgga atgacgaccg aaacttgcag ggaactatcg
     9721 gtcaagaggt gttctcgggg tagaactcct tgagcagggg gccggctatg ttctcgacca
     9781 gccttgcctg ccggggcgtg aggtcctcac gccagccacc aggcgtggcc ttgcggtaga
     9841 acttgccctc gcctttgttt ttctccggga tgttctccca ggcgtgcttc tcgaccactc
     9901 gggccagctt cttctcatct accgggatct cgagcgcaga gtagatgcgc ttcatcgtcc
     9961 ctaaagtatt ctccctcagc tcctcgtacc tgacaaagac tttgcggcct ctgtgtagat
    10021 cgtacgcccg ctttgcgttt ccggcgtgct gaaggtacgc tcgggagcgc acccggacaa
    10081 acgtctccgg gtcgccatct ggggccgcca ggttcttgcc tccctcgttc ctccactcgt
    10141 ggagccagtt cccctccttc gcgccatcga gcgtggaagc gaccacatca cggggatcac
    10201 ggatcaagag gatcatacga ctctccggca acgcctccat cagtagtgga gcgccagtcg
    10261 agccgttggg ctccttgata acgaggtagt cgtcggggcc gaggaggggt ctggcatacc
    10321 cggctccgtc gaggacgaag ttgcggatcg atcgggtcca accctttcgc gtcgcggccg
    10381 ccataatgaa gtctgggcgg ctcagattgg agtgctgtgc cctctcgtag aactctccaa
    10441 agagcctgcc gaccaacggc tcctcccaga cccgatgccg gtccatatcg cccatcatgc
    10501 ttcggagcca cgtgctaccg ctgcgcccgg agccgaagat ccagacaagg ttttcgggac
    10561 ttaccccgcc agcacgtgcc tccgcgctac ttctggagag cttcatcccc caactggcaa
    10621 ccgtcagagg aacctcccgg tactttccgg ccagccagag gcggcttcgg ttcagcccgg
    10681 ttcttactcg acgcgccatg ctcacgatcc tggcgccggt gtggatcttt ggcctacgta
    10741 tccggtcatg cggagtagaa ctctttgata agcggcgccg ctatgcgctc gatggtgttc
    10801 acctgatggt gagtcagatc ctcgcgccat ccgccagcct tccccttgcg gtagaacttg
    10861 ccctccccct tctgctcctc ggggatgttc tcccaggagt gcttctctac cgcccggatg
    10921 aggccctcct catcaaccgg aatacccaac tcggcgtaga ggcgcttcat cgtcgccagg
    10981 gtgtctgccc gaaggtcctc gtagcggacc accgtcttgt atccccggtg tgcgtcgtag
    11041 gctctcttg
//
LOCUS       DASSMB010000213        14034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_175259, whole genome shotgun sequence.
ACCESSION   DASSMB010000213 DASSMB010000000
VERSION     DASSMB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14034
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175259"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1717
                     /locus_tag="VFJ72_12690"
     CDS             23..1717
                     /locus_tag="VFJ72_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7480363.1"
                     /translation="MPSTNGHGPKTAVLYARVSTDEQARSGYSLAQQVEALREYARSE
                     GYRVLEEVIDPGQSGASLERPGMDRVRDLVAAGDVSVVLAQDRDRIAREPAYHYLLRK
                     EFEDHGTKIQALNDRGDDSPEGELTDGILDQLAKYERAKFAQRSRRGMLRKAREAGIC
                     SRPPYGFRMDAPGKRLVTHEPELAVICEIFRLAAEGLDLNAIQNRLYHEGIPSPTGKP
                     LWDIQMIKKLVENDAYKPHACEEVSALVSSEVAATLDPDKVYGVQWYNRQKTTTRTVS
                     EPDGNGGRRYRKSRTFRWRPREEWIAIPIELEDHPPRELVDLARAMFEASKGNEKKYL
                     SREWELRGLMRCSCGSKIGTRNTPDKGNVYHYYVCRRRSAARKAYGCTQRCIRAEPAE
                     AEIWSFVSGLLSDPKRIRLGMEKLIEQERSAGRGDPKREAKVWANQVAECARLRTAYQ
                     DQQAAGYMTLEELGERLRELEDTRRTAEAELESLEARRQREEGLEADRDALLEYMAAS
                     VPEGLDALTGEERNKVYRMLRLEITPKPEGYEVRGAFCTSQTIDAHPRPSSRTEIV"
     gene            1738..1822
                     /locus_tag="VFJ72_12695"
     tRNA            1738..1822
                     /locus_tag="VFJ72_12695"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1772..1774,aa:Ser,seq:tga)
     gene            1983..2219
                     /locus_tag="VFJ72_12700"
     CDS             1983..2219
                     /locus_tag="VFJ72_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480364.1"
                     /translation="MKRKIVILATAALLALLMLAPAAMAQSTSMESTGMESTTSGQQS
                     GSNLPSSGGPAILLPAAALLLGSGILTFAVLRRR"
     gene            complement(2326..3732)
                     /locus_tag="VFJ72_12705"
     CDS             complement(2326..3732)
                     /locus_tag="VFJ72_12705"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET7480365.1"
                     /translation="MVLLGVIVGLILGSSLLLRGSEEADREAPTKVRPRPEKPPPSKF
                     SYPGFYPARYDIDAIYKEKPRSTAGTQTVRYVNAEDEPMRSLHFRLWTNETVFTDLDG
                     GTTVSSVTVDGEKSRSSLDGTDLEVFFPKPLPRNATAQVALRFETTIPEIAAPFGHDS
                     GVSSMGVWYPILAVYDEDGWNLAPTTEFGEPYFSEVADYTVRLTLPKGLTPATTGVEK
                     DHDEAGGEQTSTYEAESVRDFALAIGEEFERVSRRVGETTINVYHRPESAIRAGRALD
                     LASSSLAFFSKTFGAYPYPEFDLVDAPLVAGTEYSTLTFANLENTQDYVFDTVVPRKV
                     SHQWWYVQVGSDQFEEPWLDESLATYSEWLYTGDAASRFPDPVAPTTPLDTSVGGFPD
                     SYTYQNVAYLRGAQLYRDLSYEIGEEKLLRGLRDYASRYRYRTVNADGLISTLSDSTG
                     EDLSSFFGSHGIAPPKKN"
     gene            complement(4228..5436)
                     /locus_tag="VFJ72_12710"
     CDS             complement(4228..5436)
                     /locus_tag="VFJ72_12710"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7480366.1"
                     /translation="MDDAVKEAADRVLHGVADRENGVPGVVAMATDRDGNFYEGAAGV
                     RELGKDQEMTTDTVFCIFSCTKAITGVTVMQLVEEGVLGLDDPAKEYAPEIADIQVLE
                     GFDDDGEPRVRPPRSDITVSQLMLHTAGFGYDFFNHDLIQYGEKRDVPSVVTGTQASL
                     NSVLLFDPGERWEYGSNIDWVGKVVEGARGRRLGEVMRERVFEPLGMDDTAVTMSDSM
                     RSRRATIHQRSEDDTLSPLSDFELPQDPEQHMGGHALYGTVGDYMKFIRMILNDGAGE
                     HGRVLKAETVERMAQNGLGALKIKGLPGAIPSLSNDAEFFPGMPKSWGYTFMINDEDA
                     PTGRPAGELGWAGLANLFYWIDRKNGVGGFWATQILPFADPVSFGGYMDFETTVYSHL
                     KSGRPSLAAL"
     gene            5922..6308
                     /locus_tag="VFJ72_12715"
     CDS             5922..6308
                     /locus_tag="VFJ72_12715"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7480367.1"
                     /translation="MEISKDLIAASSTPIVLAILAEEDSYGYAILKRVRELSGGRMEW
                     TDGMLYPVLHRLERLGHVEARWEVAKSGRRRKYYRITPGGRAQMAEERRQWQAVDATL
                     RGIWQAFSLPVPAGHPASAAPVPRGA"
     gene            6311..7681
                     /locus_tag="VFJ72_12720"
     CDS             6311..7681
                     /locus_tag="VFJ72_12720"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HET7480368.1"
                     /translation="MGAPDHGVSLEEQIERWRSYLRRRQAIHPVDVAELEDHLREQVA
                     VLVDAGLTTDEAFLVAVKRMGDLDALSREFAREHSDRLWKQLVVVPSDSGEPRARART
                     DAIVAFCLAVAAAGLIKAPALFGVQLDEDAGFYARNLSLFVLPLLTGYFVWKRRLDTG
                     ILRWMVLAFVAAGVFANVYPFGPAGYTEALTALHLPISLWLVVGIAYAGGRWGQVGGR
                     MDFIRFSGELFIYYVLIALGGGVLTAFMAMIFQSIGIDVEPFFESWLLPCGAIGAVVV
                     ASWLVEAKQSVIENMAPVLTRLFTPLFAAVLITFLGAMLWTGRGVGIERNVLIAFDLL
                     LVLVLGLLLYSVSARDPQSPPGVFDVVQVVLVISALLADAVALWDIAARITEFGFSPN
                     RVAALGENVILLVNLAWSAVLYIRFLRGRGSFTTLERWQTNYLPVYAVWAAIVVIVFP
                     PVFGYI"
     gene            complement(7742..8128)
                     /locus_tag="VFJ72_12725"
                     /pseudo
     CDS             complement(7742..8128)
                     /locus_tag="VFJ72_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557948.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8619..9392)
                     /locus_tag="VFJ72_12730"
     CDS             complement(8619..9392)
                     /locus_tag="VFJ72_12730"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7480369.1"
                     /translation="MTVNVNRIKQAWSSSRPAFGFWSTIPDSFGIELIAGLDLDYVCV
                     DQQHGVVDYASMVSMLRTIKSAGAAPIVRVPYNEPWLLMRALDAGALGVIVPMVNNAD
                     EAARAVAACRFPPEGARSYGPIRASSVIGSKDPEALADEVLCIVQIETLEGLGNVEEI
                     AATPGLDGIYIGPADLALGLGLPLDLSEDKSRHDEAVERIREACRKNNIAAGLHGTSG
                     ESAREYAEQGFSMVNVGVDYLLFPAAVRQEVHAARSQRV"
     gene            complement(9855..10607)
                     /locus_tag="VFJ72_12735"
     CDS             complement(9855..10607)
                     /locus_tag="VFJ72_12735"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7480370.1"
                     /translation="MSKTRRRGREQPAYRRLSVALRADIEEGRFKEGERLPTEAELGE
                     QYGVSRHTVRHAFQDLVADGLVDRIPGRGTFVNEISRRGKYQRSIGTLEEMLTWVGTE
                     LRVLDPIEIVEDAEAASRLALPSPEVASLAIMRLYEGTPLFVSYISLPPDIGRRMRES
                     GLPSDGPGTVIGSVERLFSLPVAGASLDITAISAPPSKVSEVMDYEPGEAILRVERLF
                     YGKDGTPLEYANSYYNPKQNSYRIELRRWSPI"
     gene            11229..12137
                     /locus_tag="VFJ72_12740"
     CDS             11229..12137
                     /locus_tag="VFJ72_12740"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET7480371.1"
                     /translation="MEIALWKRALAEFFGVFLLASIGLMVVATAITTQAYDLFGVSIV
                     FALVIMTIIMVVGAVSGAQINPAITIALAVFRKFPWREVPVYVVSQISGGVVGALVLY
                     FLYRGPITAFEKANDIVRGQPGSEMTALIFSCFAPHPAFAAALKWSPSVVGTGTALLA
                     EAFATMVLAVVVFAVTDERNAFAPSLPVFALTLGLVVGFIVAVEAPLTMAGLNPARDL
                     GPRIAISLLGWAGVAWSHAGFNWWVWTVGPIIGAVAGGAVWTFLFGSFLAARPVDPLA
                     AETEAEELHSPTPGGNPPSSARTARD"
     gene            12279..13871
                     /locus_tag="VFJ72_12745"
     CDS             12279..13871
                     /locus_tag="VFJ72_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7480372.1"
                     /translation="MAQYDYIVIGAGSAGAVVAARLAENPETEVLLLEAGGSDQDYPE
                     IADPGAWPFTLQGTEFDWQYVTTPQEHAAGRVGVVPRGKVLGGTSAINALVYVRGHSS
                     DYDNWAYWGNYGWDYANVLPAYRRLEDYEYGSNDYHGSGGPISVRRFREDNSNPVSVA
                     MMEAALEAGYPYTDDFNGARMQGAGYNSVTINKDNTRSGTFMYLRNAPSNLTITTHAQ
                     ARKLLIENGRCTGVEYSRGNGRNGGLERAEAREEVIVSGGAIDSPRLLMLSGIGPADH
                     LRQVGIDVKVDIPGVGQNLHDHLLIGVVYESAREVPKATWANHSETTLFWPDDERGVG
                     LCPGMQIQFIHIPFAPEGFTAPEEGSGYTITPGPNRVLSRGELTLRSSNPDDAPLINP
                     NYLIEDADVAAHVRGIQMSREIGEQDALKEWRKKEVLPGPERKTDAELREFVRQAAST
                     IYHPVGTCKMGIDHMAVVDPDLRVYGVEGLRVADASIMPAITSGNTNAPCIMIGEKCA
                     EIILSGVGVDEVSRTHSVRDAG"
BASE COUNT         2631 a         4262 c         4454 g         2687 t
ORIGIN      
        1 gccgatctcc tggaggacta acatgccctc cacaaacggc cacggcccga agacggccgt
       61 cctctacgcc cgcgtctcga cagacgagca ggcccgcagc ggctactccc tcgcacagca
      121 ggtggaggct ctcagagagt acgcacggag tgaaggctat cgggtgctcg aagaggtcat
      181 tgaccccgga cagagcggag cgtccctgga gaggcccggc atggaccgtg tgcgggacct
      241 ggtagccgcc ggcgacgtct ccgttgtgct agcacaggat cgggaccgca tcgcgcggga
      301 gccggcctat cactacctgc tgcgtaagga attcgaggat cacggaacga agatccaggc
      361 gcttaatgat cgcggcgatg atagcccgga aggagagctt accgacggca tcctggacca
      421 gctcgccaag tacgagcggg ccaagttcgc gcagaggagc cggcggggga tgctacgtaa
      481 ggccagggag gcgggcatat gttcgaggcc tccttacggc tttcgcatgg atgctccggg
      541 taagcgcttg gtaacgcacg agcccgagct ggcggtgatc tgtgagatat tccgcctggc
      601 cgctgagggc ttggatctta atgccataca gaaccggcta tatcacgagg ggattccttc
      661 tcctacgggt aagccgctgt gggacatcca gatgataaag aagctcgtgg agaatgatgc
      721 ctacaagcca cacgcctgtg aggaggtgtc tgcgctggtc tcttccgagg tggcggccac
      781 actcgacccc gacaaggtct acggcgtaca gtggtacaac cgccagaaga cgacgacgcg
      841 caccgtctcc gaaccggacg gcaacggcgg gcgacgctac cgcaagtcga ggactttccg
      901 ctggcgtccc cgcgaagagt ggatcgccat acccatcgaa cttgaagacc acccgccccg
      961 cgaattggtg gatctggctc gcgccatgtt cgaggccagc aagggcaacg agaagaagta
     1021 tctgtcgagg gagtgggaac tcagaggact tatgcggtgc tcttgcggat cgaagatcgg
     1081 cacgcgcaac acgccggaca aaggcaacgt gtaccactac tacgtctgta ggcgccgcag
     1141 tgcggccagg aaggcttacg gctgtactca gcggtgcatc agggctgagc cggccgaggc
     1201 ggagatatgg agctttgtct cagggctgct cagtgacccg aagagaatac ggctcggcat
     1261 ggagaagctt atagagcaag agaggtccgc cggacgcgga gatccaaaac gagaggcgaa
     1321 ggtgtgggcg aaccaggtgg cagagtgcgc ccgtctgaga accgcctatc aggaccagca
     1381 agcggccggg tatatgaccc tggaggagct gggagagaga ctccgggagc ttgaggacac
     1441 ccgccggacc gcggaagccg aactggagtc cctggaggcc cgcagacagc gcgaggaagg
     1501 cctggaggcg gaccgggacg ccctgctaga gtacatggcc gcaagcgtgc ccgagggcct
     1561 ggacgcgctc acgggagagg agcggaacaa ggtctatcgg atgctgaggt tggagatcac
     1621 gccaaagcca gaggggtacg aggtaagagg cgctttttgt acttctcaaa cgatagatgc
     1681 ccatcctcga ccgtctagcc gaactgagat cgtataaact tcgcggagac accgaccggg
     1741 ggcatggcag agcggacgaa tgcgctggtc ttgaaaacca gaggtggtaa aacacccgta
     1801 ggttcgaatc ctactgcccc cgcttccaca gggagaccaa tacttccaaa atattaattt
     1861 tattaaaaat tgttaagata atagagactt ttgcgttttg agtatgtaca ctacgggtaa
     1921 ttctcacgag agaagacgtg caggccgcgt ggaacgattg agtagacaga aggaggaact
     1981 ctatgaagag aaagatagtt attttggcta cggccgctct tctggcgctg cttatgctcg
     2041 cgccggcggc tatggctcag agcaccagca tggaaagcac cggtatggag agcacgacct
     2101 ctggtcagca gagtggcagc aacctgccgt cgagcggtgg tccggcgatt cttctgcccg
     2161 cggccgctct gctcctgggt tcgggcatcc tgacgttcgc cgttctacgg cgcaggtagt
     2221 acccagtaaa attacctaga cgccgttcct tcaggggagc ggccgtcgtt gagccggggg
     2281 agagcctgca ctcccccggc tattttcgta tcagcgcttc cggggttagt tcttcttcgg
     2341 gggcgcgatg ccgtgggatc caaagaaaga ggagaggtct tcgccggtgc tatcggagag
     2401 ggtggagata aggccgtcgg cattcaccgt ccggtaacgg taacggctag cgtagtcccg
     2461 gagtcctcga agtagcttct cttcgccgat ctcgtatgag aggtctctgt agagctgggc
     2521 tccgcggagg taggcgacgt tctggtaggt gtagctgtcc gggaagccgc cgacggacgt
     2581 atccagcggg gtagttggcg cgacggggtc cgggaagcgg ctcgcggcgt cgccggtgta
     2641 gagccactcg gagtaggtgg cgagagactc atcgagccag ggttcctcga actggtcgct
     2701 accgacctgt acgtaccacc actgatgaga taccttgcgc gggacgacgg tgtcaaagac
     2761 gtagtcttgc gtattctcca ggtttgcaaa ggtcaaggta gagtactcgg tgccggctac
     2821 gagcggggcg tctaccagat cgaactccgg gtacgggtaa gcgccgaagg tctttgagaa
     2881 aaaagcgagg ctgctggagg cgagatccag agcccgtccg gcccggatgg cggattcggg
     2941 acggtggtag acgttgatcg tggtctctcc gactctccgc gagacgcgct cgaactcttc
     3001 gccgattgcc agggcgaagt ctcgcacgga ttcggcctcg taggtagagg tctgctctcc
     3061 gccggcctcg tcgtggtcct tctcgactcc tgtggtggca ggggtcaggc ccttcggaag
     3121 ggtcaagcgt accgtgtagt ccgcgacctc ggagaagtac ggctcgccga actcggtcgt
     3181 cggcgcgagg ttccatccat cctcgtcgta tacagcgagg atcgggtacc agactcccat
     3241 cgaagagacg ccggagtcgt gaccgaaggg agcggctatc tctggaatag tggtctcgaa
     3301 gcgcagtgcg acctgcgccg tggcattcct ggggagaggt ttcgggaaga agacctcgag
     3361 gtcggtgccg tcgagggagg agcgggactt ctcgccgtcc acggttacgg aggagacggt
     3421 agtgccgccg tccaggtccg tgaagacggt ctcgttcgtc cagaggcgga agtggagcga
     3481 gcgcatcggt tcgtcttcgg cgttcacata ccggacggtc tgcgtacccg cggtggatct
     3541 cggcttctct ttgtagatgg cgtcgatgtc atagcgggcg ggatagaatc cagggtaaga
     3601 gaacttcgaa ggtggaggct tctccggccg gggtcggacc ttggtggggg cctcccggtc
     3661 tgcttcctcg cttccccgga gcaggagaga gcttcccaga atcaggccca cgataacgcc
     3721 cagcaatacc aacagcgccg cgatccgccg cctgcggtac gtccgtttga ggttgaggga
     3781 tcgaacggag gctctccggg agaatgatgg atcagagcca gagaagccgg gggtcccgcg
     3841 actccggcgc ggacgaggcg aaccggagga tcttcttcgc ttcaattctc gctcggctgg
     3901 ttcggagacg gtcaacggtt ccccctctgt acctggctcc agactcatgg tacattcgcg
     3961 agtcttcgcg agcaatggaa gcgaggtccc ggagcaccgg tgtcagtcgg gtggcgcggc
     4021 tacgatgctt ctccccggat cacgacgggc gccacctcct ggagatcagc ccggcgcctt
     4081 gagcgcgaaa ccgcttaagc aggcatcttt tcgacgcttt gccggttttt accactgatt
     4141 cgcggacggc ggctccggga cacgtgtccc ggagccgctt ctcgtcaggg acctgaaggc
     4201 cggtgagtcc ggtctccacc cgcgcttcta aagagcggcc aggctcggac ggccggattt
     4261 caggtggctg tagacggtgg tctcgaagtc catgtagccg ccgaaggaga ccgggtccgc
     4321 gaacgggaga atctgggtcg cccagaaacc gccgacgccg ttcttacggt cgatccagta
     4381 gaacaggttc gcgagcccgg cccagcccaa ctcgcccgcc ggccgcccgg ttggagcgtc
     4441 ctcgtcgttg atcataaagg tgtatcccca ggacttcggc atgccgggga agaactccgc
     4501 gtcgttggac aggctcggga tggcgccggg gaggcccttg atcttcaacg ctcccagtcc
     4561 gttctgagcc atgcgctcta ctgtctccgc tttcagcacc cggccgtgtt cgccggctcc
     4621 gtcgttcaag atcatgcgga tgaacttcat gtagtcgccg accgtgccgt acagggcgtg
     4681 gccgcccatg tgctgctcag ggtcctgcgg cagctcgaag tccgagagcg ggctcagggt
     4741 atcgtcctcg ctgcgttgat gaatggtcgc gcgccgggaa cgcatcgagt cggacatcgt
     4801 gaccgcggta tcgtccatcc cgagcggctc gaagacgcgc tcgcgcatca cctcgccgag
     4861 acgcctgccg cgtgccccct cgaccacctt gccgacccag tcgatattgc tcccgtactc
     4921 ccagcgctcg cccggatcga agagcaagac ggagtttagt gacgcctgcg tgccggtgac
     4981 gacgctcggc acgtctctct tctcgccgta ctggatcagg tcgtggttga agaagtcgta
     5041 gccgaagccc gcggtgtgca gcatcagttg gctcaccgtg atatcggagc ggggcggacg
     5101 gacgcgcggc tcgccgtcat catcgaagcc ttcgagcacc tggatgtcgg cgatctccgg
     5161 cgcgtactcc ttcgcgggat cgtcgaggcc gagcacgccc tcctcgacca actgcatcac
     5221 cgttacgcct gtgatcgcct tagtgcagga gaagatgcag aatacggtgt ccgtggtcat
     5281 ctcctgatcc ttgccaagct cgcggacgcc cgcggcgcct tcgtagaagt tcccgtcgcg
     5341 gtcggtcgcc atagccacca cgccgggcac gccgttctct cggtccgcca cgccgtgaag
     5401 cacgcgatcg gcggcctctt ttacagcatc atccatattt tcctctttcc cacagccagg
     5461 cttgcgctct ctgtcctcgt accccctgca acaggcgcat catagcaccc gctcaggtta
     5521 gaggtcgcgc ggcccagggt tatcgtcttc aacggtcctt ctgaagtccg ccacacagca
     5581 ggacgctctt ttgccaggcg cgcgccgaga gattgagcct gacgtgccgg agcggctcct
     5641 tggcgctact tcaagttatc caaatccgga cacgcttggt tacggctctg atccagttgc
     5701 cgatccgtta cagcagagcg aggtacagaa tcttccggta gagtgccgga aactctttga
     5761 gccatcacgt tttagctgca aacatagact ttttaaagtg agtcgcccag gactcgaacc
     5821 cacggctctt tatatacata acactatcat gtatagttct gctatgtata cttgggtgag
     5881 ttcattagcg tcgcgcgacg cgtgaaatga tggagaaaat catggagatc agcaaggacc
     5941 tgatagccgc ttcctcgacc ccgatcgtcc tggcgatcct ggccgaagag gacagctacg
     6001 gctacgcgat cctcaagcga gttcgggagt tgtccggagg gcgcatggaa tggacggatg
     6061 ggatgctcta ccctgttctg catcggcttg agcggctcgg ccacgtcgag gcgcggtggg
     6121 aagttgcaaa gagcggtcgt cgtcgcaagt actaccggat cacgcctggg ggccgggctc
     6181 agatggcgga ggaacgcagg caatggcagg cggtggacgc tacgctacgg ggcatctggc
     6241 aggcgttctc ccttcccgtt ccggccggcc accccgcgtc ggccgcgcca gtgccacggg
     6301 gagcctgacg atgggggctc cagatcacgg ggtatcgctc gaggagcaga tcgaaaggtg
     6361 gcggagctac ctccgccgcc ggcaggcgat ccaccccgtc gatgtagcgg agctggaaga
     6421 ccatctgcgc gagcaggtgg cggtcctggt cgacgcggga ctcaccaccg atgaagcgtt
     6481 cctggtggcg gtgaagcgca tgggcgacct cgacgccctc tcacgggagt tcgcgcgcga
     6541 gcactcggac cgcctctgga agcagctcgt cgttgtcccc tccgattccg gggagccacg
     6601 agcgcgggcc cgaacggatg ccatcgtcgc cttctgtctt gcggtggcgg cggccgggct
     6661 catcaaggcg ccggcgctct tcggcgttca gttggacgaa gacgccggct tctacgcccg
     6721 caatctgagc ctcttcgtgc tgccactcct gaccggctat ttcgtctgga agcggcggct
     6781 cgacaccggc atcctccgct ggatggtcct ggcgttcgtc gcggcgggtg tattcgccaa
     6841 cgtctatcct tttggcccgg ccggctacac cgaggcgctc acggcgctgc acttgccgat
     6901 ctcgctctgg ctggtggtgg gcatcgcgta cgccggcggc cggtggggcc aggttggggg
     6961 ccgcatggac ttcattcgat tctcgggcga gctgttcatc tactacgtgc tgatcgccct
     7021 cggcggcggc gtgctcacgg cgtttatggc gatgatcttc cagtccatcg ggatcgacgt
     7081 cgagcccttc ttcgagtcgt ggctgctgcc gtgcggcgct ataggggccg tcgtggtcgc
     7141 ctcctggctg gtggaggcca agcagagcgt gatcgagaac atggcgcccg tgttgacgcg
     7201 cctcttcacg ccccttttcg ccgccgtgct catcacgttt ctgggagcca tgctgtggac
     7261 cggacgtgga gtcggcatcg agcggaacgt gctcatcgcc ttcgacctgc tcctggtgct
     7321 ggtcctcggc cttctgctct attccgtctc cgcccgggac ccccagtcgc cccccggcgt
     7381 cttcgacgtg gtgcaggtcg tgctggtgat cagcgcgcta ttggccgacg cggtagcgct
     7441 gtgggacatc gccgcgcgta tcaccgagtt cggcttcagc cccaaccgcg tggccgccct
     7501 gggtgagaac gtgattctcc tggtcaatct ggcgtggtcc gccgtgctct acatccgctt
     7561 tctgagaggg cgcggatcgt tcacgaccct cgaacggtgg cagacgaatt acttgccggt
     7621 ctacgccgtg tgggcggcga tcgtcgttat cgtctttccg ccggtgttcg ggtacatctg
     7681 atcgggtgcc gccgcacccg ttctccgcag cgacattcgc cttgcggatt ggcgggtcga
     7741 ccatcacgta gggccgtccc cgttcttcga gccatcctcg caaacctcgg gccgtcccgt
     7801 agaccgtgtc ggcgacgatc caccgcgccg ggacgtcggc ctcgaaggcc cgctccagta
     7861 tccgcttggc gagtcccctt ggttgcaaac cgcaccccct cgggaacgcc ggcttcggct
     7921 cggcgctctt cgtcccccgc ccaatctttg ggtaggtata gggcacgggg tccacgaagg
     7981 ccgcaccttt gcgcgaggcg tagaccgcga acacttctac ctgcgagttc tctatccggc
     8041 ccgccgttcc cgtgtactgg cgggcgacgc ctgcggactt ctcgcccttc ttgaagaagc
     8101 ccgtctcgtc cacgatgagc acccgctctc ctcgtctccc aggcgctcgg agacgtaccc
     8161 gcgcaggtcg ccgcgcacta cggcgtcgcg cacggcgtcc caacgagcgg cgttaaagag
     8221 gcgctgcgca ccttgcgctc cacgcgttcg agcaggctgg cgaggtagcg cctcgctcgc
     8281 tccctgacct acggcctcgg gaagcggcac gcgatgtggg catgcaaccc gccaagggca
     8341 tcgggacatc gttccacagc ggcgaattcg ccgcctccgg tcttgtagtt ctccgcgccg
     8401 acatgctgct cctctaaccg gttagaggag cagcacaccg cagctcaata gaagtgtcgc
     8461 tgtaggatta gtagccgcaa aagcggagaa cttctcggca gtcggcacat ctcccagggg
     8521 cacataagat acacagcgaa gatcccatct ttaccgaagc tgccattctt ggcatctacg
     8581 ggagtacggg atgccaaagc ttcgtcgaga ctcgaacgtc aaacccgctg gcttcgagcc
     8641 gcgtgtacct cctggcgaac cgcagcgggg aacaacagat agtctactcc cacgttgacc
     8701 atggaaaatc cctgctccgc gtactctctg gccgactccc ccgaggtgcc gtgcagaccc
     8761 gcggcgatgt tgtttttccg gcaggcctct cgtatacgct ccacggcctc gtcgtgcctg
     8821 gacttatctt ccgacaggtc gagtggaagt cccaggccca gggcaaggtc cgcagggcct
     8881 atgtagatac cgtcgagtcc cggagtggcg gcgatctctt ccacgttccc gagaccttct
     8941 agagtctcga tctggactat gcacaagacc tcgtcagcca aagcttccgg gtccttcgag
     9001 ccgatgacgc tggaggcccg gatcggaccg taggaacgag cgccctcggg cggaaagcgg
     9061 cacgccgcta cagcccgcgc agcttcgtca gcattgttga ccatgggaac gatcacgccc
     9121 aacgctccag catccagcgc cctcattaac agccagggct cgttgtacgg cacgcgcacg
     9181 atgggggcgg cgccggcgga cttgatcgtt cggagcatgg aaaccatgga ggcgtaatca
     9241 acgactccgt gctgctggtc tacgcagacg tagtcgaggt ccaaaccagc gatcaactcg
     9301 attccaaaag agtccggtat agtggaccag aacccgaatg ccgggcgaga cgaagaccac
     9361 gcttgcttta tacggttaac gtttaccgtc atcaaagtac ctcctaaaag ggttagagag
     9421 ctcacaccat gctacaacga ggcccccgga ctcccctcgc tggagagaac cgcgttgact
     9481 gcgggcttcc caaaccgggt ccgtggggcg ccctgactaa agcgcagcgt tttggactga
     9541 agacgccaac cagcgggcgt cggttgtggt gatcgttcaa cacatggaat cccggtgaac
     9601 gtccaaatgc tcataggagc gttcccgtcg cacctcctac tcgtacgtct ttcgacctct
     9661 cttacgttga gcggagaact tcgcgctgcg gacatgaccg caaccgccct ccaatatcaa
     9721 ccggatcaat catgtacgta caaatcgcgt ctgtcaatgc cactatctgt aaggaccttg
     9781 cacgagcgat tcaggaccta cgtaaaaaag ggaagcggat ggtgcgtgtt gcgtgttgga
     9841 tccagggtcc cgaattagat cggtgaccag cggcgcagct ctatacggta ggagttctgt
     9901 ttcgggttgt agtacgagtt ggcgtactcc aggggggtgc cgtctttgcc atagaagagg
     9961 cgctcgactc gcaggatcgc ctctccgggc tcgtagtcca tcacctccga gactttagaa
    10021 gggggagcgg agatagccgt gatatcgagg ctggctccgg cgacggggag cgaaaagagg
    10081 cgttccacgc taccgataac ggttccgggg ccatcagatg gcaggccgga ctcccgcata
    10141 cgcctcccaa tatctggagg aagagagatg tagctcacga agagcggagt cccttcatag
    10201 agcctcataa tcgcgaggct tgctacctcc ggagacggca gcgccagacg ggatgccgct
    10261 tcagcgtctt cgacgatctc tatcgggtcc aacacacgca gctccgtacc gacccacgtc
    10321 aacatctcct cgagcgtgcc gatcgagcgc tggtacttcc cgcgccttga aatctcgttg
    10381 acgaaggtgc cacgtccggg gattcgatca accaggccgt cggcgaccag atcctggaac
    10441 gcatgtctca cggtgtgccg gctaacgcca tattgctcac ccaactctgc ctctgtcggt
    10501 aagcgttcgc cttccttgaa gcgtccttcc tctatgtctg cgcgaagtgc taccgaaagt
    10561 cgccggtaag ccggctgttc gcgtcctcgc cgcctggtct tactcagtct ttctcacctc
    10621 cgcctttatc taggatcaga gtctttcgct gcattgcacg gatcgttgct ctaagtaaac
    10681 aaaatatacc atccgacttc cgccacgggc attacatctt tggatgtatg tctattcggt
    10741 gcatcgcttg gctgatgaat ctgtgtacgg ttgatagtcg tcggtttctc cgaagcagcc
    10801 tgctgccgcc acagatccaa tatgggttga tcagttgcgc aaaggatcgg cctcttcgag
    10861 cctccatgtt atgagatttt ctgactggaa ccaaccccta tcggtagacc aacttacatg
    10921 gatggagtgt cggaactgtc gagactgcac gggttcactg ccttctcccg ttttctaaac
    10981 catcagcaaa tttatcggca aatgctattg actgagttat gtacgtacat tatcctcgtc
    11041 gacataccat aatgtacgta cattatccgg tataggggaa cggaagcgag aggaggagag
    11101 cgtggtcgat gcctgaacgt ttgcccatcg ttggcttggg attttggttt gaggtggtgt
    11161 tcatccgctg ggtaggtggg tggcagagta gcagtcagca gtatcgcgga cgttagggag
    11221 gtattgaggt ggagattgct ctatggaaac gggcgttagc cgagttcttt ggcgtgttct
    11281 tgttggcctc gataggcttg atggtcgtcg cgaccgcgat cacgacccag gcgtacgacc
    11341 tgttcggggt gtccatcgtc ttcgcgttgg tgatcatgac catcatcatg gtggtcggtg
    11401 cggtgagtgg agcccagatc aacccggcca tcaccatcgc gctcgccgtg ttccggaagt
    11461 tcccgtggcg cgaggtgccg gtttacgtcg tgtcccagat ctccggtggg gtggtcgggg
    11521 cgctggtact ctacttcctc taccgggggc ccatcacggc gttcgagaag gccaacgaca
    11581 tcgtccgggg ccagcccggg agcgagatga ccgccctgat cttctcctgc ttcgcgccgc
    11641 acccggcctt cgccgccgcc ctcaagtgga gtccgagcgt tgtggggacg ggcaccgcgt
    11701 tgctcgccga ggcctttgcc accatggtgc tggccgtcgt ggtgttcgcg gtgacggacg
    11761 aacggaacgc gttcgcgccg agcctgcccg tcttcgccct taccctcggg ctcgtcgtcg
    11821 gctttatagt cgctgtggaa gcgccgctga ccatggccgg tctcaacccc gcacgtgacc
    11881 tcgggccgcg catcgcgatc tccctcctgg gctgggccgg cgtcgcctgg tcccatgccg
    11941 ggtttaactg gtgggtgtgg accgtgggac caataatcgg ggcggtcgcc ggcggtgccg
    12001 tgtggacgtt cctgttcgga agtttcctcg ccgcgcgtcc cgtggacccg ctggccgccg
    12061 aaaccgaggc cgaggaactg cactccccga cgccgggtgg caatcccccc tcttccgcca
    12121 ggacggccag ggactagacc aataaagagt gtttgcggcg cgcaaagaac acgagcgtcc
    12181 cgcaggcgaa aggaggccct cacggcgacg aggttagcaa agacgcaagt tgaacatcga
    12241 ttgatttcgg aacagacaaa caaggaggat tagccaccgt ggcgcagtac gactatatcg
    12301 tgatcggcgc tggttcggct ggcgccgtgg tagcagcccg gctcgccgag aacccggaga
    12361 cggaggtctt attgttggag gcgggcggtt cggatcagga ctacccggag atcgcggacc
    12421 ctggagcctg gccgttcacc ttgcagggca ccgagttcga ctggcagtac gtgaccacgc
    12481 cgcaggagca cgccgccggg cgcgtcggcg tcgtgccgcg cggcaaggtg ctgggcggca
    12541 ccagcgccat aaacgcgctg gtgtacgtgc gcggtcacag cagcgactac gacaactggg
    12601 cctactgggg caactacggc tgggactacg ccaacgtgct gcccgcgtac cgcaggctgg
    12661 aagattacga gtacgggtca aacgattacc acggctccgg cgggccgatc tccgtccgcc
    12721 gcttccggga ggacaactcc aacccagtct ccgtggcgat gatggaagca gcgctggagg
    12781 ctggttaccc gtacaccgac gacttcaacg gggccaggat gcagggcgcc ggctacaaca
    12841 gcgtgaccat caacaaggac aacacgcgct ccggcacctt tatgtacctc aggaacgcgc
    12901 cctcgaacct tacgataacc acccacgctc aggcgcgaaa gttgctcatc gagaacggac
    12961 gttgcacggg ggtcgagtac tcgcggggca acggacgaaa cggcgggctc gaacgcgccg
    13021 aagcacggga agaggttatc gtctccggcg gcgccataga ctcgccccgg ctgttgatgc
    13081 tctcgggtat cggaccggcg gatcacctgc ggcaggtggg catagatgtc aaggtggata
    13141 tcccaggcgt cgggcagaac ctgcacgacc acctgctcat tggcgtcgtc tacgagtcgg
    13201 ccagggaggt gccgaaggcc acgtgggcca accactcgga gacgacgttg ttctggcccg
    13261 acgacgagcg cggggtcggg ctgtgccccg gcatgcagat ccagttcatc cacataccct
    13321 tcgcccccga gggcttcacg gcgccggagg aaggcagcgg ctacacgata acgccgggcc
    13381 cgaaccgcgt cctcagccgc ggcgaactca cattgcgctc gtccaacccc gatgacgccc
    13441 cgcttatcaa cccgaactac ctcatagaag atgccgacgt ggcggcccac gtcaggggta
    13501 tccagatgtc gcgcgagata ggcgagcagg acgctctcaa ggagtggagg aagaaggagg
    13561 tgctgccggg accggagcgg aagaccgacg ccgaactgcg cgagttcgtg cgccaggcgg
    13621 cgagcaccat ctaccatccg gtcggcacct gcaagatggg catagaccat atggccgtcg
    13681 tggacccgga cctgcgcgtg tacggcgtgg aggggctgcg cgtcgcggac gcctcgatca
    13741 tgccggctat cacgtcgggc aacaccaacg cgccgtgcat catgatcggt gagaagtgcg
    13801 ccgagatcat cctctcgggc gtgggcgtcg acgaggtgag ccggacccac agcgtccgcg
    13861 acgccggatg aggcgccgaa cggcacgctg aaagctagcg caccgtagct ggcagggaac
    13921 cgcgccccac ttggaacgcg gttccctgcc agcgggaggt agagatcata cgaccaaccg
    13981 ggaggactct ctcttgaaag cagccgttct cgaagcggcg aaacgcctcg tcat
//
LOCUS       DASSMB010000214        15465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_816926, whole genome shotgun sequence.
ACCESSION   DASSMB010000214 DASSMB010000000
VERSION     DASSMB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 15465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15465
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816926"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /gene="tuf"
                     /locus_tag="VFJ72_12750"
     CDS             <1..151
                     /gene="tuf"
                     /locus_tag="VFJ72_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7480373.1"
                     /translation="EGVEMVMPGDNTVMNVELISPIAMDEGLNFAIREGGRTVGAGVV
                     TEIVE"
     gene            214..363
                     /gene="rpmG"
                     /locus_tag="VFJ72_12755"
     CDS             214..363
                     /gene="rpmG"
                     /locus_tag="VFJ72_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7480374.1"
                     /translation="MRQLVTLACEECKRRNYSSRKNRRNTPDRIQLQKFCPWCRQHTA
                     HRETR"
     gene            377..449
                     /locus_tag="VFJ72_12760"
     tRNA            377..449
                     /locus_tag="VFJ72_12760"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:410..412,aa:Trp,seq:cca)
     gene            480..743
                     /gene="secE"
                     /locus_tag="VFJ72_12765"
     CDS             480..743
                     /gene="secE"
                     /locus_tag="VFJ72_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET7480375.1"
                     /translation="MGKKRASAAQAKGTNKKSQQQPKKSFFSGIRDFVRGVRAELKRV
                     SWPNRDQLRQSTAVVLIIVLVLGAYVAAWDFVFQSIARLVFLR"
     gene            759..1295
                     /gene="nusG"
                     /locus_tag="VFJ72_12770"
     CDS             759..1295
                     /gene="nusG"
                     /locus_tag="VFJ72_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET7480376.1"
                     /translation="MKKWYVINTYSGHENKVRTTLERRVESLGVGRHFGEISIPMESV
                     IEIKDGKKVPVEQRQFPGYILVNMDMNENTWQIVRQTPGVTQIVGNGDKPLPLSRAEV
                     ERLLHPGEAADAAREKVKTTVDYTIGETVKVIGGPLSDFTGAISDINVDQSRLKVLVS
                     IFGRETPVELSFNQVAKL"
     gene            1317..1754
                     /gene="rplK"
                     /locus_tag="VFJ72_12775"
     CDS             1317..1754
                     /gene="rplK"
                     /locus_tag="VFJ72_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET7480377.1"
                     /translation="MGRGRGRRVARKLKLQITGGQANPAPPVGPALGQAQVNIGEFVR
                     QFNDATRDQMGQVVPVEITVFEDRSFTFITKTPPAAFLLKQAAGLEKGSGEPNSNKVG
                     SVSRAQVEEIARTKMPDLNAADIDGAAKIVEGTARSMGITVNG"
     gene            1747..2436
                     /gene="rplA"
                     /locus_tag="VFJ72_12780"
     CDS             1747..2436
                     /gene="rplA"
                     /locus_tag="VFJ72_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET7480378.1"
                     /translation="MGKRLLEQSTKIDRERLYGPREALELLKALEGAKFDESVEAHVN
                     LNVDPRRADQMVRGTLMLPNGTGKTRRVAVFAVGEKAREAEDAGADVVGADDLAARIR
                     DENFMDFDVAVATPDQMSVVGRLGQVLGPRGLMPNPKSGTVTMDVGRAVEDIKKGKIE
                     YRVDRYGIIHSILGKKSFDLDSLVANYFALREELQRVRPAPVKGRYFKSITLTTTMGP
                     SVKVDPSVEKE"
     misc_feature    2453..2585
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2596..3123
                     /gene="rplJ"
                     /locus_tag="VFJ72_12785"
     CDS             2596..3123
                     /gene="rplJ"
                     /locus_tag="VFJ72_12785"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET7480379.1"
                     /translation="MKREDKAKVIEDLAGKLRGGSAVLVDYQGINVAQSTDLRRQSRE
                     NGVEFVVAKNTLTQRAANEAGIEGLEQYLVGPTALAFSEDPVASAKLMAKFADQVETF
                     VLKGGLLEGDRVLNETEVVALSKLPGREQLIAQIVGGISSPLTGFVTVLNNTIQGLVV
                     ALGQIAEQKQSAEQS"
     gene            3164..3547
                     /gene="rplL"
                     /locus_tag="VFJ72_12790"
     CDS             3164..3547
                     /gene="rplL"
                     /locus_tag="VFJ72_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET7480380.1"
                     /translation="MAVTKEELMESIENMTVLELSELVKALEERFGVSATAVAAAPAA
                     GAAGDGAAAAEEEQTEFDVVLTGAGAKKIQVIKEVRAATGLGLKEAKALVDEAPNPVK
                     EALSKEDAEALKAKLEEAGATVELK"
     gene            3871..7245
                     /locus_tag="VFJ72_12795"
     CDS             3871..7245
                     /locus_tag="VFJ72_12795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7480381.1"
                     /translation="MVSPIVRRKRHPYARMQTADFQVPPLVEIQINSFQKFLDEGIQR
                     TLTEISPIEDYTGSYAVEFGESYFEDPPVSIEECISKDKTYAAPLFVKVRFIIKETGE
                     LREQDVFMGDFPLMTQSGTFIINGTERVVVTQLVRSPGAYIMEPKDVSKQVLTASLMP
                     SRGSWLEFEVEQKGYISVRIDRKRKLPVTVLLKALDMGGPEELLSRFGDSWLIRNTLE
                     KDDTASRDDALLEVFRRQRPGEPVNIENAQNLVDGLFFEPKRYDLSEVGRYKVNKKLK
                     LDVPMETQTLTIEDIEGLLKRLMEISEEVAEDEEFGRINYERIRHKLDEYEHFGNRRL
                     RTVGELVQEAFRIGMYRMERVVRERMTTQDTDTITPQTVVNTKPVASAIKEFFGSSQL
                     SQFMDQTNTLSGLSHRRRLSAMGAGGLSRERAPIEVRDVHPTHYGRMCPIETPEGPNI
                     GLIGQLSTYATVDKYGFVQTPYRKVVDGKVTDEIEYLSADEEEELTIAQANTPVDIKT
                     GKIEEGAQILARRRGGDVATVSPEEVDYMDVAPLQTVSVGTALIPFLEHDDANRALMG
                     ANMQRQAVPLLRSQAPYVGTGMEFRAAADTGDVLLARNAGTVERVVADRISVRREDGE
                     VDDYRLRKFARSNQGTCVNQRPLVTEGESIEAGTVLADGSSTDEGELALGKNLLVAFM
                     PWEGYNFEDAIIISERIVKDDVLTSIHIEEYEVQARDTKLGPEEITRDIPNASDDVLM
                     NLDIDGIIRIGAEVGSGDVLVGKVTPKGESEPTPEEKLLRAIFGEKAREVKDSSLKVP
                     HGEGGVVIDVKRFSRDDGDELQPGVNEMVRVFVAKKRKIAEGDKLAGRHGNKGVISKI
                     VPEEDMPYMEDGRPVDVILSPLGVPSRMNIGQILETHLGWAASQGLGENGGEPTFVST
                     PVFSGAEVEDIDKAIEKVRTTTGAGVKMGRGGKVTLRDGRTGEPFDGRITVGYMYILK
                     LLHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAHTLQELLT
                     IKSDDRVGRVKSYESIVKGENIPEPGVPASFKVLLKEMQSLGLSVKPVYSAEAAAEDT
                     ERRDWDEAARALGINLSREEPTGNLDDAPDFGRGGM"
     gene            7249..11133
                     /locus_tag="VFJ72_12800"
     CDS             7249..11133
                     /locus_tag="VFJ72_12800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET7480382.1"
                     /translation="MQGLERDIDINKLEKISIGLVSAEEIREWSRGEVTKPETINYRT
                     LRPEKDGLFCERIFGPSKDWECYCGKYKRIRFKGIICERCGVEVTRARVRRDRMGHVE
                     LAAPIAHVWFVKGVPSRMGYLLDISPKDLDRVLYFASSIVTWVDREERANDIDMLRER
                     VEEEIRQLEEDRDEEVMQTQALRTKRESVIKGESSPDELTEEYEDIPEEEREAAIAKV
                     HKEAEEAVADVQRSITEQIELVRRSWEEFQTLEPRQIVDDEELFREMKDRFGDEYGYG
                     VYFRGGMGAEAVRDLITQVDLEDEAEELRSVVSDSKGQKRQKAIKRLKVVDAFRTSGN
                     KPEWMIMDAVPVLPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLRRLLDLGAPDV
                     IVNNEKRMLQEAVDALFDNGRRGRAVTGAGNRALKSLSDMLKGKQGRFRQNLLGKRVD
                     YSGRSVIVAGPNLKMHQCGLPRLMAVELFKPFVMKVLVDRALAPNIRSAKQMVERLRP
                     EVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVEGKAIRVHPLVCSAFNADFDGD
                     QMAVHVPLSPEAQAEARVLMLSTQNILLPANGFPIAVPSQDMVLGLYYITYTDEGFED
                     AEVKGFISSYDEAEAAYKEGTLKLHDKVITRRNGERMETTLGRVLFNENIEQALRDYL
                     GDSYDPASYPYVNEVLKKGEIKDLVSDYVDRYPTELVSQLLDTLKRVGFHTATKAGLS
                     VGKNDIVVPVQKEGILETYQAKVDEIEDQWDQGFISDDESLERTIGLWTQAKNEVEQA
                     MKDNLYRLNPIYMMANSGARGNYSQITQLAGMRGLMTNTKGEIIPEPVKSNFMEGLSV
                     LEYFTSTHGARKGQADTALRTADSGYLTRRLVDVSQDVVVNGEDCGSDGYIEIPLYLN
                     GGRGDANDSVAARYLARDLVNPETGEVVAEAGTDITRKILDGWREQLPRETMVPVRTP
                     SKCENAHGVCQACYGRALSTYRPVDVGEAVGIIAAQSIGEPGTQLTMRTFHTGGIAGE
                     DITAGLPRVVELFEARHPKAEATLADIDGIVSLEEADSDRLIKVVITSSDNTESREYK
                     VERQLLRPEFVEGAEVLANTPITDGSVYPHQILENDLRYDRGTTATERYIVEEVQRVY
                     RTQGVDIHDKHIEVIVRQMLRKVVVDDPGDTNFLPEQVADRFAVRAENERVEEEGGKP
                     ATFHTILMGITKASLATDSFLSAASFQETARVLTDAAIEGKVDNLAGLKENVIIGKLI
                     PAATGLPRYRQLQVTVEGYKAREIDELDIAAS"
     gene            11251..11619
                     /gene="rpsL"
                     /locus_tag="VFJ72_12805"
     CDS             11251..11619
                     /gene="rpsL"
                     /locus_tag="VFJ72_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7480383.1"
                     /translation="MPTTNQLVRNERKLKKEKTATPALQSSPQRRGVCTRVSTTTPKK
                     PNSALRKIARVRLTNGMEVTAYVPGEGHNLQEHSVVLVRGGRVKDLPGVRYKVVRGAL
                     DTLGVNNRKQGRSKYGTKKA"
     gene            11631..12101
                     /gene="rpsG"
                     /locus_tag="VFJ72_12810"
     CDS             11631..12101
                     /gene="rpsG"
                     /locus_tag="VFJ72_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7480384.1"
                     /translation="MPRRAAPPKKKIEADPVYGSVLVQQVINKIMLDGKKSTAEKIVY
                     DALSFIEERTGTSPVQVLNNALDNIRPRLEVRSRRVGGATYQVPVEVIPRRANTLALR
                     WMVGFARARREKTMGRRLAGEILDAKDNIGSSVKRKEDTHRMAEANRAFAHYRW"
     gene            12113..14263
                     /gene="fusA"
                     /locus_tag="VFJ72_12815"
     CDS             12113..14263
                     /gene="fusA"
                     /locus_tag="VFJ72_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7480385.1"
                     /translation="MAVQVAKKIPLKRVRNIGIMAHIDAGKTTTTERILLYTGLTHKL
                     GEVHDGNAVMDWMAQERERGITITSAATTVFWGGIEGSGKNGKGQREGVHSRIPDQHR
                     INIIDTPGHVDFTVEVERSLRVLDGSIALFDSVAGVEPQSETVWRQADKYGVPRIAFV
                     NKMDRIGADFYKAVGTMVDRLGANAVPVQLPIGAESEFQGIVDLVEMRAIVYTDDLGA
                     TWEITDIPEDLRTLAEEYREKLLEAVADQDEELMMMYLEGEQIEVEQLRSAIRKATLD
                     NQMTPVFVGSAFKNKGVQPLLDGVVDYLPSPLDVPPIQGKTLDGEETVTREADENGPL
                     SALAFKVQSDPHVGKLTYMRVYSGTLKSGSYVMNTTKGKRERIGRLLQLHANSREQRE
                     EVYAGELVAAIGLSDTGTGDTLISADDPEQIVLEEMIFPEPVISQAIEPKTKADQEKL
                     SVALQRLAEEDPTFSVRGDEETGQTIISGMGELHLEIIVDRLLREFRVDANIGRPQVA
                     YRETIRKRVEGINGRFVRQTGGRGQYGHAVINAEHNEETGYLFENKIVGGVIPREYIG
                     SVDKGIKEALDSGVVAGFPVVDVRVELVDGSFHEVDSNEMAFQIAGSMAIKEAMKRAD
                     PVLLEPIMSVEVVMPEEFMGDVMGDLSSRRGQIQGMDARGGGQVIRAMVPLSEMFGYA
                     TTVRSKTQGRATFTMQFDHYAEVPKSIAAEIAER"
     gene            14273..>15465
                     /gene="tuf"
                     /locus_tag="VFJ72_12820"
     CDS             14273..>15465
                     /gene="tuf"
                     /locus_tag="VFJ72_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7480386.1"
                     /translation="MSKGKFERNKPHLNVGTIGHVDHGKTTLTAAITKVLAKRVSDDP
                     ANKIVAFDQIDNAPEERQRGITIATSHQEYATANRHYAHVDCPGHADYVKNMITGAAQ
                     MDGAILVVSAADGPMPQTREHILLARQVGVPYIVVFLNKADMVDDEELLELVEMEVRE
                     LLTEYDFPGDDIPVVTGSALKALEGDEGELGEEAIMQLMEAVDTYVPEPERAIDRDFL
                     LAVEDVFTIQGRGTVATGRVETGEIRLNEEIEIVGIRPTRKTVVTGVEMFNKSMDYAQ
                     AGDNIGALLRGVKRDDIERGQVLAKPGTITPHTRFKAEVYVLSKEEGGRHTPFFSNYR
                     PQFYFRTTDVTGEIRLEEGVEMVMPGDNTVMNVELISPIAMDEGLNFAIREGGRTVGA
                     GVVTE"
BASE COUNT         3217 a         4481 c         5276 g         2491 t
ORIGIN      
        1 agaaggagta gagatggtga tgcccggaga caacacggta atgaacgtgg agctcatctc
       61 gcccatagct atggatgagg gtctcaactt cgcgatacgt gagggtggca ggactgttgg
      121 tgctggtgtc gtcaccgaga tagtcgagta ggtaaaaagc ctgacggatg aggggcggtg
      181 cggtgtgccg cccctgtgat ttggaggaat gttgtgcgac aattagtaac gctggcttgc
      241 gaggagtgca agcgccggaa ctacagcagc cgtaagaacc ggcggaatac tccggaccgc
      301 atccagctac agaagttctg tccgtggtgc cggcagcata cggcgcaccg ggagacccgt
      361 taagggaccg agacagaggg cagtagctca gttggaagag cagcggtctc caaaaccgca
      421 ggtcgtgggt tcgagtccct cctgccctgc tccagtagat agaaccaggc gaggaagtga
      481 tgggtaaaaa gcgcgcctcc gcggctcagg ccaaggggac caacaagaag tcacagcagc
      541 agcccaagaa gagtttcttc agcgggatca gggatttcgt gcgcggggtc cgggccgagt
      601 tgaagcgggt tagctggccg aaccgggatc agctccggca gagcacggcg gtcgtgttga
      661 tcatcgtgct ggtgctcggc gcttacgtgg ccgcgtggga cttcgtcttc cagagcatcg
      721 cccgcctggt gttcctaagg tagcgggagt taagagattt gaagaagtgg tacgtcataa
      781 acacatactc ggggcacgag aacaaggtcc gcacgacgct tgagcggcgg gtcgagtctc
      841 tcggtgtagg ccgtcatttt ggagagatca gcatcccgat ggagagtgtc atcgagatca
      901 aggacggcaa gaaggtcccc gtcgagcagc gccagttccc cggatatatc ctggtgaaca
      961 tggacatgaa cgagaatacc tggcagatcg tgcgccagac gccgggcgtc acccagatcg
     1021 tcggcaacgg agacaaaccc ctgccgctga gccgcgccga ggtcgagcgt ctgctgcatc
     1081 cgggcgaggc cgccgacgcg gcgcgggaga aggtcaagac gacggtggat tacactatcg
     1141 gggagacggt caaggtcatc ggcggaccgc tctcggactt tacgggtgcg atctcggaca
     1201 taaacgtgga ccagtcgcgg ttgaaggtgc tggtctcgat cttcggccgg gagacgccgg
     1261 tcgagctttc tttcaatcag gtagcaaaac tctaacaatc cgaggagagg tttcttatgg
     1321 gtagaggacg cgggcgccgc gtagcgcgca aattgaagtt gcagatcacg ggcggtcagg
     1381 cgaaccccgc cccgccggtc ggcccggcgc tgggccaggc tcaggtgaat atcggggagt
     1441 tcgtgcggca gttcaacgac gccacgcgcg accagatggg gcaggtcgtc cccgtggaga
     1501 tcacggtctt cgaggaccgc tctttcacgt ttattaccaa gacgcccccg gcggcgttcc
     1561 tgctgaagca ggccgcgggt ctggagaagg gcagcggcga gccgaacagc aacaaggtcg
     1621 gctccgtcag ccgggcgcag gtcgaggaga tagccaggac aaagatgccg gacctcaacg
     1681 ccgccgatat agacggcgcg gcgaagatcg tcgagggcac ggcccggagc atggggataa
     1741 ccgtcaatgg gtaagcgact tttggaacag agcacgaaga tcgaccgcga gcgtctctac
     1801 ggaccgcgcg aggcgctgga gttactcaag gcgctcgaag gcgcgaagtt cgacgagagc
     1861 gtcgaggcgc acgtcaacct gaacgtggac ccccgacgcg ccgaccagat ggtgcgcggc
     1921 accctgatgc tcccgaacgg caccggcaag actcgccggg tggctgtctt cgccgtgggt
     1981 gagaaggccc gcgaggcgga ggacgccggc gcggacgtgg tgggcgccga cgacctggcg
     2041 gcacgtatcc gggacgagaa cttcatggac ttcgacgttg cggtcgcgac gccggaccag
     2101 atgagcgtgg tcgggcgtct cgggcaggtg ctgggaccgc gcgggctgat gccgaacccg
     2161 aagagcggca cggttacgat ggacgttggc cgcgcggtcg aggatataaa gaagggcaag
     2221 atcgagtacc gggtggaccg ctacggcatc atccactcca tcctcggcaa gaagtccttc
     2281 gacctggata gcctggttgc caactacttc gccctgcggg aggagcttca gagggtgcgt
     2341 ccggcgccgg tgaagggccg ctacttcaag tccattacgc tcacgaccac gatgggaccg
     2401 tcggtcaagg ttgacccttc cgtcgagaag gagtaatatc tcgcgcggac tttaagttaa
     2461 taagagtaga ccgaagacag ccggggtccg agaggactta atcatccggc cgaggttgcg
     2521 gaaagcttta cgggatcttt ttagccccga gcgcctctgc ggcctcgggg ttttacattt
     2581 ggcggaggag ttgaggtgaa gagggaagac aaggctaagg tgatcgaaga cctcgccggg
     2641 aagcttcgcg gcggctcggc ggtactcgtg gattatcagg gcatcaacgt ggcgcagagc
     2701 accgacctga gacgtcagag ccgcgagaac ggcgtcgagt tcgtagtcgc caagaacacg
     2761 ctgacgcagc gggcggctaa cgaggccggc atcgaggggc tggagcagta cctggtcggg
     2821 ccgacggcgc ttgcgttctc cgaggacccg gtggcgagcg cgaaactgat ggcgaagttc
     2881 gcggatcagg tcgagacttt cgtcctgaag ggcggtctgc tggagggcga ccgggtgctg
     2941 aacgagaccg aggtcgtggc tctgagcaag ctgccgggcc gcgagcagct catcgcccag
     3001 atcgtcggcg gcatctcgtc gccgctcaca ggcttcgtga cggtgttgaa caacaccatc
     3061 cagggcctgg tcgtggcgct cggccagatc gcggagcaga agcagagcgc cgaacagtcg
     3121 tagttcagta gtaggcaagt aagtagattc aaggaggaag aagatggctg taaccaaaga
     3181 ggagttgatg gagtcgatcg agaacatgac cgttctcgag ctgtcggagc tggtgaaggc
     3241 tctggaggag cgcttcggcg tctccgccac cgccgtcgcg gctgccccgg cggccggcgc
     3301 ggccggcgac ggcgcggctg ccgccgagga ggagcagacc gagttcgacg tcgtgcttac
     3361 cggcgcgggc gcgaagaaga tccaggtcat caaggaggtg cgcgccgcca ccggactcgg
     3421 cctgaaggag gccaaggctc tcgtggacga ggctccgaac cccgtaaagg aagccctctc
     3481 caaggaggac gccgaggccc tgaaggccaa gctggaagag gccggcgcca ccgtcgaact
     3541 gaagtaactc cgtcgctccc cgcctaccgg cttttgccgg agacgccggg gttcgattgc
     3601 atctacgaga ggcgctgccg cattgctgcg gtggcgtctc ttttgcgcgg tggtgccgat
     3661 tggcgtggta gcaagaagcc gatagctcca tccgtggcac attgcggagg gttatgctat
     3721 attgcccggc gtcggtgagc agttcgactg gcgaaaaata tttttgatca gaacgcggtt
     3781 cgcgcgtctt gacttgccct ctccgcgggg gtaagatatt cgtctgcgtg tttaaccgtt
     3841 cttcaacaac cctgccggag gagaactaac ttggtaagtc ccattgtgcg ccgcaagcgg
     3901 cacccctatg ctcggatgca gaccgccgat ttccaggttc cgcccctggt cgagatccag
     3961 ataaactctt tccagaagtt tcttgacgaa gggatccagc ggacgctgac ggagatctct
     4021 ccgatagagg attacactgg ctcttacgcc gtggagttcg gggagtctta cttcgaggat
     4081 ccaccagtct ctatagagga gtgcatctct aaggacaaga cttacgcggc tccgctcttc
     4141 gtgaaggtcc gcttcatcat aaaggagacg ggagagctcc gggagcagga cgtgttcatg
     4201 ggggacttcc cgctcatgac tcagtcgggg acgttcataa taaacgggac cgagcgcgtc
     4261 gtcgtaacgc agctcgtgcg ttctccgggc gcgtacatta tggagccgaa ggacgtctcc
     4321 aagcaggtcc tgacggccag cctgatgccg agccgtggct cctggctgga gttcgaggtc
     4381 gaacagaagg gctatatctc cgttcgcatc gaccgcaagc ggaagctgcc ggtaacggtc
     4441 ttgctcaagg cgctcgacat gggcgggccg gaggagttgc tgtcgcggtt cggggactcg
     4501 tggctcatac ggaacacgct ggagaaggac gataccgcgt cgcgggacga cgcgctcctg
     4561 gaggtcttcc ggcgccagcg tcccggcgag ccggtcaaca tcgagaacgc ccagaacctg
     4621 gtggacgggc tgttcttcga gcccaagcgc tacgatctct cggaggtcgg acgctacaag
     4681 gtcaacaaga agctcaagct cgacgtgccg atggagactc agacgctcac catagaggac
     4741 atcgagggcc tgctcaagcg cctcatggag atctccgagg aggtcgccga ggacgaggag
     4801 ttcgggcgca tcaactacga gcgcatccgg cacaagctcg acgagtacga gcacttcggc
     4861 aaccggcgtc tcaggactgt cggggagttg gttcaggaag cgttccggat cgggatgtac
     4921 cggatggagc gggttgtgcg cgagcgcatg accacgcagg atacggatac catcacgccg
     4981 cagaccgtcg tcaacaccaa gccggtcgcc agcgcgatca aggagttctt cggctcctcg
     5041 caactctcgc agttcatgga ccagacgaat actctctccg ggctttcgca ccggcggcgc
     5101 ctcagcgcga tgggcgcggg cggtctcagc cgggagcggg ctccgataga ggtccgggac
     5161 gtgcatccga cgcactacgg gcggatgtgc ccgatcgaga cgccggaggg accgaacatc
     5221 gggctcatcg ggcagctctc cacctacgcg acggttgaca agtacggctt cgttcagacg
     5281 ccgtaccgca aggtcgtcga tgggaaggtg accgacgaga tcgagtacct gtcggccgat
     5341 gaggaggaag agctcaccat cgcgcaggcc aacacgcccg tcgatatcaa gaccggcaag
     5401 atcgaggagg gagcccagat cctggctcgg cgtcgcggcg gcgacgtggc taccgtctcg
     5461 cccgaggaag tggactacat ggacgtggcc ccgctccaga cggtctccgt cgggacagcg
     5521 ctgatcccgt tcctggagca cgacgacgcg aaccgggcgc tcatgggcgc gaacatgcag
     5581 cgtcaggccg tcccgctgct ccgcagccag gccccgtatg tcgggaccgg tatggagttc
     5641 cgggccgcgg cggatacggg cgacgtgctg ctggcgcgta acgctggtac cgtggagagg
     5701 gtcgtggcgg acaggatctc tgtccggcgc gaggacggcg aggtggatga ttaccgtctc
     5761 cgcaagttcg cccgctccaa ccagggcacc tgcgtgaacc agcgcccgct cgtcacggag
     5821 ggcgagagca tagaggccgg cacggtgctg gcggacggct cctcgaccga tgagggagag
     5881 ctggctctcg gtaagaacct gctcgtggcg ttcatgccgt gggaaggcta caacttcgag
     5941 gacgccatca tcatctccga gcggatcgtc aaggacgacg tgctcacttc gatccacatc
     6001 gaggagtacg aggtccaggc gcgggacacc aagctgggac ccgaggagat cacgcgggat
     6061 atccccaacg cctcggacga cgtgctgatg aacctcgata tcgacggcat catccgcatt
     6121 ggcgccgaag taggctccgg cgatgtactc gtcgggaagg tcacgccgaa gggcgagtcc
     6181 gagccgacgc ctgaggagaa gctcctacga gcgatcttcg gcgagaaggc ccgtgaggtg
     6241 aaggactctt ccctcaaggt cccgcacggc gagggcggcg tcgtcataga cgtcaagcgc
     6301 ttcagccggg acgacggcga cgagctccag ccgggcgtca acgagatggt ccgggtgttc
     6361 gtggcgaaga agcgcaagat cgcggagggt gacaagctcg ccggacgtca cggcaacaag
     6421 ggcgtcatct ccaagatcgt gcccgaggag gacatgccgt acatggagga cgggcgtccg
     6481 gtagacgtta tcctgtctcc tctcggcgtg ccgagccgga tgaacatcgg ccagatcctc
     6541 gaaacccacc tgggctgggc cgcaagccag ggccttggcg agaacggcgg cgagccgacc
     6601 ttcgtctcga ccccggtctt ctccggcgcc gaagttgagg acatcgacaa ggccattgag
     6661 aaggtccgca ccaccacggg agccggggtc aagatgggcc gcggcgggaa ggtcacgctg
     6721 cgcgacggcc gcaccggaga gccgttcgac ggcaggatca cggtcggcta tatgtacata
     6781 ctgaagctct tgcacctggt ggacgacaag atccacgctc gttcgaccgg tccgtactcg
     6841 ctggtcacac agcagcccct gggcggcaag gcccagttcg gcggccagcg gttcggcgag
     6901 atggaggtgt gggcgctgga ggcatacggc gcggcgcaca ccttgcagga gttgctcacc
     6961 ataaagagcg acgaccgcgt gggccgggtc aagagctacg aatcaatagt gaagggcgag
     7021 aacataccgg agccgggcgt tccggcgagc ttcaaggtgc tcttgaagga gatgcagtct
     7081 ctcgggctgt cggtcaagcc ggtctacagc gccgaagctg cggccgaaga caccgagcgg
     7141 cgcgactggg acgaggctgc gagggcgctg gggatcaacc tcagccgcga ggagcctacc
     7201 gggaacctgg acgatgcgcc ggacttcggc agaggaggga tgtaagcgtt gcagggacta
     7261 gagcgcgaca tagacataaa taaacttgag aagatctcca tcggcctcgt ttcggccgag
     7321 gagatccggg agtggtcccg cggcgaggtc acgaagccgg agaccatcaa ctaccgcacc
     7381 ctccgcccgg agaaggacgg cctgttctgc gagcggatct tcggaccctc aaaggactgg
     7441 gagtgctact gcggcaagta caagcgcatc cgcttcaagg gcattatctg cgagcgctgc
     7501 ggcgtggagg ttacccgcgc ccgcgtgcgc cgcgaccgga tgggccacgt ggagcttgcc
     7561 gctccgatag cgcacgtctg gttcgtgaag ggcgtgccga gccggatggg ttatctgctc
     7621 gatatcagcc cgaaggacct ggaccgggtg ctgtacttcg cctcgtctat cgttacctgg
     7681 gtggaccggg aggagcgggc gaacgacatc gacatgctcc gcgagagggt cgaggaggag
     7741 atccgtcagc tcgaagagga ccgggacgag gaggtcatgc agacccaggc gctccgcacc
     7801 aagcgcgaga gcgtcataaa gggcgagtcc tccccggatg agctgaccga ggaatacgag
     7861 gacatcccgg aggaggagcg cgaggcggcc atcgcgaagg tccacaagga ggccgaggag
     7921 gccgtcgccg acgtgcagcg ctccatcacc gagcagatag agctcgtgcg gcgctcctgg
     7981 gaggagttcc agacgctgga gccccgtcag atcgtggacg acgaagagct gttccgcgag
     8041 atgaaggacc gcttcggcga cgagtacggc tacggcgtgt acttccgcgg cggcatgggc
     8101 gccgaggccg taagagacct catcacccag gtagacctcg aagacgaggc cgaagagttg
     8161 cggagcgtgg tgagcgattc aaagggccag aagcgccaga aggcgataaa gcgcctcaag
     8221 gtcgtggacg ccttccgcac gagcggcaac aagccggagt ggatgatcat ggatgcggtc
     8281 ccggtgctgc cgccggactt gcgcccgatg gtgcagctcg atggcggacg cttcgccacg
     8341 agcgacctga acgacctgta ccgcagggtc atcaaccgga acaaccgtct gcgccgtctg
     8401 ctggacctgg gcgcgccgga cgtgatcgtg aacaacgaga agcggatgct ccaggaggcc
     8461 gtggacgccc tcttcgacaa cggccgccgc ggacgcgccg taacgggcgc cggcaaccgg
     8521 gcgctcaagt cgctctccga tatgctcaag ggcaagcagg gccgtttccg gcagaacctg
     8581 ctcggcaagc gggttgacta ctcgggccgc tcggtcatcg tggccggccc gaacctcaag
     8641 atgcaccagt gcggtctgcc gcgtctcatg gcggtcgagc ttttcaagcc gttcgtgatg
     8701 aaggtgctcg ttgaccgggc gctcgctccg aacatccgga gcgcgaagca gatggtcgag
     8761 cggctccgtc ccgaggtctg ggacgtgctg gaggacgtga tcaaggagca ccccgtgctc
     8821 ctgaaccgcg ccccaacgct ccaccgcctc ggcatccagg ctttcgagcc ggtgctggtc
     8881 gagggcaagg ccatccgggt ccatccgctg gtgtgctccg cgttcaacgc cgacttcgac
     8941 ggcgaccaga tggcggtcca cgtgccgctc tcgcccgagg ctcaggccga ggcgcgggtg
     9001 ctgatgctct cgacgcagaa catccttctc ccggcgaacg ggttcccgat cgcggtcccc
     9061 tcgcaggaca tggtgctcgg gctctactac atcacctaca ccgacgaggg gttcgaagac
     9121 gccgaggtca agggatttat ctcttcctac gacgaggccg aggccgcgta caaggagggc
     9181 acgctcaagc tgcatgacaa ggtcatcacg cgccgtaacg gcgagcggat ggagaccacg
     9241 ctgggacggg tgctgttcaa cgagaacatc gagcaggcgc taagagatta cctgggcgac
     9301 tcgtatgacc cggcgtctta tccttacgtc aacgaggtgt tgaagaaggg tgagatcaag
     9361 gacctcgtct ccgactacgt ggaccgctat ccgacggagt tggtcagcca gcttctcgac
     9421 accctgaaga gggtcgggtt ccacacggcc acgaaggccg gcctctccgt cggcaagaac
     9481 gacatcgtgg ttccggtgca gaaagagggc atcctggaga cctaccaggc gaaggtcgat
     9541 gagatcgaag accagtggga tcagggcttt atctccgatg acgagagcct ggagcggacc
     9601 atcggcctgt ggacgcaggc caagaacgag gtcgagcagg cgatgaagga caacctgtac
     9661 cgcctgaacc cgatctacat gatggctaac tccggcgcgc gagggaacta ctcgcagata
     9721 acgcagctcg ccggcatgcg cggcctgatg acgaacacga agggcgagat catccccgag
     9781 cccgtgaagt ctaacttcat ggaaggcctc tcggtcctcg aatacttcac ctccacacac
     9841 ggcgcccgga agggccaggc cgacacggcc ctgcgtaccg ccgactcggg ctatctgacc
     9901 cgccgtctag tcgacgtctc tcaggacgtc gtggtcaacg gcgaggactg cggctcggac
     9961 ggatacatcg agataccgct ctacctcaac ggcggccggg gcgacgccaa cgactctgtc
    10021 gccgcccgtt acctggcgcg cgacctggta aatccggaga ccggcgaggt cgtcgccgag
    10081 gccgggacgg acatcacgcg caagatcctg gacggttggc gcgagcagtt gccacgcgag
    10141 acgatggtgc cggtccgcac gccgtcgaag tgcgagaacg cccacggcgt ctgccaggcg
    10201 tgctatgggc gcgcgctctc gacctaccgt cccgttgacg tgggtgaggc cgtcggcatc
    10261 atcgcggccc agtccatcgg cgagccgggg acgcagctta cgatgcgtac cttccacacc
    10321 ggcggtatcg ccggcgagga catcacggcc ggtcttcccc gggtggtcga gctcttcgag
    10381 gcccggcacc cgaaggccga ggcgactctg gccgacatcg acggtatcgt ctcgctggaa
    10441 gaggccgact ccgaccggct catcaaggtg gtaatcacgt cttcggacaa tacggagagc
    10501 cgggagtaca aggtcgagcg ccagctcctg aggcccgagt tcgtcgaggg cgcggaggtg
    10561 ctggcgaaca ccccgatcac cgatggctcc gtctacccgc atcagatcct cgagaacgac
    10621 ctgcgctacg accgcggcac gaccgccacc gagcgctaca tcgtcgagga agtgcagagg
    10681 gtgtaccgga cgcagggcgt ggacatccac gataagcaca tcgaggtgat agtccgccag
    10741 atgctgcgca aggtcgtggt ggatgacccc ggcgacacga acttcctgcc ggagcaggtc
    10801 gctgaccgct tcgccgtgcg cgccgagaac gagcgcgtgg aggaagaggg gggcaaaccg
    10861 gccaccttcc acactatctt gatgggtatc acgaaggctt cgctcgccac cgatagtttc
    10921 ctctccgcgg cttcgttcca ggagacagcg cgcgtcctta ccgacgccgc aatcgagggc
    10981 aaggtggaca acctggcggg cctcaaggag aacgtcatca tcgggaaact tatcccggcc
    11041 gctaccggac tgccgcgata ccggcaactc caggtgacgg tcgagggcta caaggcccgc
    11101 gagatcgacg agcttgacat agcggcctcc tgagcctaaa attatgcgtc gcggttacga
    11161 gcgagttact aagcgtagca agctcgatga tcgcggcgcg taattttgtt gtagttcctt
    11221 ttttcaagag ttagagagga aggcgcggcc atgccgacga ctaatcagct cgtgcgtaac
    11281 gagcgcaagt tgaagaagga gaagacggcg actccggcgt tgcagagctc tccgcagcgg
    11341 cgcggcgtgt gtactcgcgt ctcgacgacg acaccgaaga agccgaactc tgcgctgcgg
    11401 aagatcgcgc gcgtgcggct gacgaacggt atggaggtta cggcctacgt tccgggcgag
    11461 ggccacaacc tgcaggagca ctccgtggtg cttgtgcgcg gcggccgagt gaaggacctg
    11521 ccgggcgtgc gctacaaggt cgtgcgcggc gcgctggata ctctcggagt caacaaccgc
    11581 aagcagggcc gttccaagta cggcaccaag aaggcgtagg aggataatag atgccgaggc
    11641 gagcagcgcc cccgaaaaag aagatagagg cggacccggt ctacgggagc gtgctggtgc
    11701 agcaggtcat caacaagatc atgctggacg gcaagaagag taccgccgag aagatcgtct
    11761 acgatgctct ctcgttcatc gaggagcgca ccgggacgag ccccgtccag gtcctcaaca
    11821 acgccctcga caacatccgg ccgcgcctgg aagtgcgcag ccgcagggtc ggtggcgcga
    11881 cgtaccaggt gccggtcgag gtgatcccgc gccgggcgaa tacgctggcg ttgcgctgga
    11941 tggtcggctt cgcgcgggcg cgccgggaga agacgatggg ccgcaggctg gccggcgaga
    12001 tccttgacgc gaaggacaac atcggctcca gcgtgaagcg caaggaagac acgcaccgga
    12061 tggcagaggc gaaccgtgct ttcgcacact acaggtggtg acgaggtaga gaatggctgt
    12121 acaggtagct aaaaagatcc cgctgaagcg ggtacggaat atcggcatca tggcccacat
    12181 cgacgccggc aagacgacga cgacggagcg catcctgctc tacacgggcc tcacccacaa
    12241 gctgggcgag gtccacgacg gtaacgccgt gatggactgg atggcccagg agcgcgagcg
    12301 cggcatcacg atcacgtccg ccgcaacgac cgtcttctgg ggcggtatcg aaggctccgg
    12361 caagaacggc aagggccagc gggagggcgt ccatagccgt attcccgatc agcaccgtat
    12421 aaatattata gacacgcccg gacacgtgga cttcacggtc gaggtggaac ggtcgctgcg
    12481 ggtgctcgac ggctccatag ctctgttcga ctccgtggcg ggtgtggagc cgcagtcgga
    12541 gacggtgtgg cggcaggccg acaagtacgg tgtgccgagg atcgcgttcg tcaacaagat
    12601 ggaccgcatc ggggctgact tctacaaggc ggtcggtacg atggttgacc ggctgggcgc
    12661 gaacgccgtt ccggtgcagt tgccgatagg cgcggagagc gagttccagg ggatcgtgga
    12721 cctcgtggag atgcgggcca ttgtgtacac ggatgatctg ggggcgacgt gggagatcac
    12781 ggacatcccc gaggacctgc ggactctcgc cgaggagtac cgggagaagc tgctcgaagc
    12841 ggtcgccgac caggacgaag agttgatgat gatgtacctg gagggtgagc agatcgaggt
    12901 ggaacaactt cggtccgcta ttcgcaaggc tactctggat aaccagatga cgcccgtatt
    12961 cgtcggctcg gccttcaaga acaagggtgt ccagccgctg ctcgacggcg tggttgacta
    13021 cctgccgagc ccgctcgacg ttccgccgat tcagggcaag acgctggacg gtgaggagac
    13081 cgtaacgcgc gaggccgacg agaacggtcc gctgtcggcg ctcgccttca aggtgcagag
    13141 cgacccgcac gtcgggaagc tgacgtacat gagggtgtac tccggcacgc tcaagtccgg
    13201 ctcctacgtc atgaacacga cgaagggcaa gcgcgagcgc atcggacggt tgttgcagct
    13261 acacgccaat agccgggagc agcgcgagga agtgtacgcg ggcgagcttg tggcggccat
    13321 cgggctctcg gacaccggga cgggcgatac gctcatctcg gcggacgacc ccgagcagat
    13381 cgtgctggag gagatgatct tcccggagcc ggtaatctcg caggccatcg agcctaagac
    13441 gaaggccgat caggagaagc tgagtgtggc gctccagcgt ctcgccgagg aggacccgac
    13501 gttctccgtg cgcggcgacg aggagacggg gcagacgatc atctccggta tgggcgagct
    13561 tcacctggag atcatcgtgg accgtctgct gcgggagttc cgggtggacg cgaacattgg
    13621 ccgtccgcag gtggcgtacc gcgagaccat tcgcaagcgt gtcgagggca tcaacggccg
    13681 tttcgtgcgg cagaccggtg gtcgcggcca gtacggtcac gcggtcatca acgccgagca
    13741 caacgaagag actggctact tgttcgagaa caagatcgtc ggcggcgtca tcccgaggga
    13801 gtacatcggg agcgtcgaca agggcatcaa ggaggcgcta gatagtggcg tcgtggccgg
    13861 gttcccggtc gtggatgtca gggtcgagct ggtagacggt tcgttccacg aggtcgactc
    13921 caacgagatg gcgttccaga tcgcgggtag catggcgatc aaggaagcca tgaagcgcgc
    13981 cgatccggtg ctcttggagc cgatcatgag cgtcgaggtc gtcatgcccg aggagttcat
    14041 gggcgacgtc atgggcgacc tgtcgtctcg ccggggacag atccagggta tggacgctcg
    14101 cgggggcggt caggtcatcc gggcgatggt cccgctctcc gagatgtttg gttacgcgac
    14161 gaccgtccgc tccaagactc agggccgcgc gacgttcacg atgcagttcg atcattacgc
    14221 cgaggtgccg aagagcatcg cggccgagat agctgaaagg taggaggaga gtatgagcaa
    14281 gggcaaattt gagaggaaca agccgcacct gaacgtgggg acgatcgggc acgtagatca
    14341 cggcaagacg acccttaccg cggcgatcac gaaggttctt gcaaagcggg tctctgacga
    14401 cccggccaac aagatcgtcg ctttcgatca gatagacaac gctcccgagg agcgccagcg
    14461 cggcatcacc atcgcaacct cccaccagga gtacgccacg gccaataggc actacgccca
    14521 cgtcgactgc cccggacacg ccgactacgt aaagaacatg atcaccggag cggcccagat
    14581 ggacggggcg atcctcgtgg tatcggcagc cgacggcccc atgccccaga cgagagagca
    14641 catcctcctg gccaggcagg taggggtgcc atacatagtg gtgtttctaa acaaggccga
    14701 catggtagat gacgaggagc tcttagagct tgtagagatg gaggtcaggg agctcctcac
    14761 cgagtacgac ttccccggag acgacatccc cgtggtcacg ggctctgcgc tcaaggcgct
    14821 cgaaggagac gaaggggagc tgggcgaaga ggcgatcatg cagctgatgg aagcagtaga
    14881 cacgtacgtg ccagagcccg agcgagccat agaccgcgac ttcctcctgg cggtagaaga
    14941 cgtcttcacc atccagggtc gagggacggt agccacgggt agggtggaga ccggagagat
    15001 aaggctaaac gaagagatag agatcgtagg gatacggccc acaagaaaga cggtagtaac
    15061 gggagtagag atgttcaaca agagcatgga ctatgcccag gccggagaca acataggagc
    15121 actactccga ggagtcaagc gcgacgacat agagcgcggg caggttctgg ccaagcccgg
    15181 aaccatcacg ccccacaccc gcttcaaggc cgaggtgtac gtgctctcca aggaagaagg
    15241 aggaaggcac accccgttct tctccaacta ccggccgcag ttctacttcc gcacgacgga
    15301 cgtcaccggc gaaattcgtc tggaagaagg agtagagatg gtgatgcccg gagacaacac
    15361 ggtaatgaac gtggagctca tctcgcccat agctatggat gagggtctca acttcgcgat
    15421 acgtgagggt ggcaggactg ttggtgctgg tgtcgtcacc gagat
//
LOCUS       DASSMB010000215         3236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_817071, whole genome shotgun sequence.
ACCESSION   DASSMB010000215 DASSMB010000000
VERSION     DASSMB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_817071"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VFJ72_12825"
     CDS             complement(<1..304)
                     /locus_tag="VFJ72_12825"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HET7480387.1"
                     /translation="MAVPARERGNEQWLAELRGPERDRAIADLRVLLLRGLRAALHGR
                     TSGIDPSMEDFAQEALIKILDNLDSFRGECRFTVWAQKIAVRTAFAEIRHSRWRDVS"
     gene            complement(368..1135)
                     /locus_tag="VFJ72_12830"
     CDS             complement(368..1135)
                     /locus_tag="VFJ72_12830"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HET7480388.1"
                     /translation="MMESIIRTVDAEVADFAAAVTRGAGEVLGDNLHAAYLSGSVALG
                     GYVPGQSDIDIMAVCRHPLPDERKRKLADAVSHVADTCPTRGLEFVLYSRGAVATPSR
                     SPRFEINLNAGPEMPYHLSLDPASEPSHWFVLDVSIAREHGLRLAGPPSHEVFAPIPR
                     SWLLDALEDSLVWHAEHETLTHYSVLNACRAWRFAEEGVWSSKEAGAKWARPRVEDPT
                     VIDAALEIRRGQARRLDPSEVRAFVMGVRSRVARIPR"
     gene            1396..1722
                     /locus_tag="VFJ72_12835"
     CDS             1396..1722
                     /locus_tag="VFJ72_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480389.1"
                     /translation="MKRLEFGAGIRVCPFCETGELCTYGLLTRCDSCDRIVEEAVLKI
                     MEQIAALPDALGKHACECGHPEMRRLPDRVFHCPACGSEVVPTKPDALLRNSQESVNG
                     GLREAA"
     gene            1769..2815
                     /locus_tag="VFJ72_12840"
     CDS             1769..2815
                     /locus_tag="VFJ72_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480390.1"
                     /translation="MKRAATLFSALLSGTTIALLLIAVVFAASATAAAQILPSATNLN
                     AAQKTITLPLFKGKTATGQNTWYVVTESSSRADATRRGVNYAPKLKNALGTKSVQKVT
                     VSGGIVRFPGTVDFRPVRQVVPGPTGFPPDVAEPGSVGDANYSNLISTGNGIVLNAEQ
                     IANNTGRHDKVVAINFKARRVTVQLTEGRQGGHKVLYLSTDSSDRGAATLEASTYAPN
                     LNAAPGVADDDVDTSARTGLVAIVNEATGVNNPQRQGLSSAILDGQDPLNILQWPPTN
                     DRYSPLWDVHPAVWTDAAIKAGERERLSSFDQVAGEVGSGNIVSGGAGPSNDKLNGLK
                     AGGFIVNCPIIVTF"
     gene            2913..>3236
                     /locus_tag="VFJ72_12845"
     CDS             2913..>3236
                     /locus_tag="VFJ72_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HET7480391.1"
                     /translation="MRIEEASQTTEQISPDGHRGEDGSGYQPIENYGIIGDLHTTALV
                     GTDGSIDWLCLPRHDSPSVFAAILDDGKGGRFKIAPDMEGATCKQLYWPDTNVLVTRF
                     FTPDGV"
BASE COUNT          641 a         1035 c         1024 g          536 t
ORIGIN      
        1 gcgagacatc gcgccacctg ctgtgccgta tctcggcgaa ggctgttcta acggcgatct
       61 tctgcgccca caccgtgaag cggcactctc cgcggaatga gtccaggttg tccagtatct
      121 tgatgagcgc ctcctgggcg aagtcctcca tggagggatc tataccgcta gtccggccgt
      181 gcagggcggc tctcaatccg cgaaggagca agacgcgcag atccgcgatg gccctgtctc
      241 tctccggccc tcgcagctcc gcgagccact gctcgttgcc acgctctctc gcagggacgg
      301 ccataatgca tcatcttacc ggcgttacac gccgaccgcg acctccgacg ccccgtgtcg
      361 ggcgaggtca acgagggatg cgcgcgacgc gagacctgac ccccatcacg aaggcgcgca
      421 cctcggaggg gtccaggcgt cgcgcctggc cgcgacggat ctccagtgcc gcatctatta
      481 cggtggggtc ttccacacgc gggcgcgccc acttcgcacc ggcctctttc gaggaccaga
      541 cgccctcctc ggcgaagcgc catgcccggc aggcgttcag cacgctgtag tgggtaagcg
      601 tctcgtgttc ggcgtgccac accagagagt cttccagggc atccagcagc caggaccggg
      661 gtatcggcgc gaacacctcg tgggaaggag gaccggcgag ccgcagcccg tgctcccgcg
      721 cgatggagac gtccagcacg aaccagtggg acggttccga ggccgggtcg agcgagagat
      781 ggtacggcat ctccgggccg gcgttcaggt tgatctcgaa ccgcggcgac cgtgacggcg
      841 tcgcgaccgc tccccgagag tagagcacga actccagtcc gcgggtcgga caagtgtccg
      901 ctacatggga gacggcgtcc gcgagtttcc gcttccgctc gtccggcaga ggatgtcggc
      961 agaccgccat gatgtctatg tcgctctggc cggggacgta gccaccgagc gccaccgacc
     1021 ccgacaggta agctgcgtgg aggttgtctc ccaggacttc cccggcgccg cgtgtgacgg
     1081 cggcggcgaa gtccgcgact tcagcgtcta ctgtccttat tatagattcc atcatcccgg
     1141 atcatccctc gtatctcgaa ggtttcaatg ctctctgtca ataagagcgc ctggcgggcc
     1201 gactccttac ttatagcctc agcgaatcct atgcttcgtc ccgctctcgt ggtcggtggt
     1261 gtgagccggt tccaattccg acccacccgg ccgcacgaaa aaatcttcag gagcaggtaa
     1321 gaacccggcc cctcgaatac tcgatagtag tgatgggagt tacttgatgg ttgggcagcg
     1381 aggaaaggat cggagatgaa acgcctggag ttcggagccg gtatccgggt ctgtcccttc
     1441 tgcgagacgg gcgaattgtg cacctacggg ttgctgaccc gatgcgattc ctgcgaccgg
     1501 atcgtcgagg aagccgtgct gaagatcatg gaacagatag ccgcgttgcc ggacgccctc
     1561 gggaagcacg cctgcgagtg cggccatccc gagatgcggc gtctcccgga cagggtgttc
     1621 cactgcccgg cgtgtggttc ggaagtcgtt cctacgaaac cggatgcact cttaaggaat
     1681 tcgcaagaat cggttaatgg tggcttaaga gaagcagcct aacttctgcg tagcaagtgt
     1741 tggcaagcgc caggaaggag caagacagat gaaacgcgca gcaactttgt tttcggcttt
     1801 gctatcgggg acgactatcg ccctcctgct gatcgcggtg gtgttcgcgg cgtccgcgac
     1861 agccgcggcc cagatcctgc cgagcgcaac caacctcaac gcggcacaga agaccatcac
     1921 gctgcccctc ttcaagggca agacggccac cggacagaac acctggtacg tcgtaaccga
     1981 gtcctccagc cgcgcggacg ccacccggcg cggcgtcaac tacgcgccca agctcaagaa
     2041 cgccctgggc acgaagtccg tgcagaaggt gacggtatca gggggaattg tgagatttcc
     2101 tgggaccgtg gacttccggc ccgtgcggca ggtagtgccc ggaccgaccg ggttcccgcc
     2161 tgacgtggcc gagccgggtt cggtcggaga cgccaactac agcaacctga tctccaccgg
     2221 caacggcatc gtgctgaacg ccgagcagat cgccaacaac accggccgcc acgataaggt
     2281 cgtagccatc aacttcaaag cgagacgggt gaccgtgcag ctcacggaag gccgccaggg
     2341 tggacacaaa gtcctctacc tcagcaccga ctcttcggat agaggcgcag cgaccctcga
     2401 agcctcgacc tacgccccga acctgaacgc ggcgccgggc gtggctgacg acgacgtgga
     2461 cacctcggcc cgtaccgggc tggtcgccat cgtcaacgag gctacgggcg tgaacaaccc
     2521 gcagcgccag gggctctcct cggcgatcct cgacggacag gacccgctca acatcttaca
     2581 gtggccgccc acgaacgacc gctacagccc gctgtgggac gtgcatcccg ccgtgtggac
     2641 cgacgcggcc atcaaggccg gcgagcggga acggctttcg agcttcgacc aggtcgcggg
     2701 cgaggtcggg agcgggaaca tcgtcagcgg cggagcggga ccgagcaacg acaagctcaa
     2761 cgggttgaaa gccggaggct ttatcgtgaa ctgtccgatc atagttacct tctagttcgc
     2821 gaaacggatg gaccgggcgg cagttcccgc ccggttcact caccacgcca acacaccaga
     2881 acgaggaact ccgcgggaga ggaagttgag acatgaggat cgaagaagca tcacagacaa
     2941 ccgagcaaat cagccccgac ggccatagag gcgaggatgg atccggctat cagccgatag
     3001 agaactacgg cattatcggc gacctgcaca ccacggcgct ggtggggacg gacggctcca
     3061 tagactggct gtgcctgccg cgccatgact cgccgagcgt cttcgcggcg atcctggacg
     3121 acgggaaggg cgggcggttc aagatagccc cggatatgga aggggctacc tgcaagcagc
     3181 tctactggcc ggatacgaac gtcctggtca cgcgcttctt tacgccggac ggggtc
//
LOCUS       DASSMB010000216         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_176202, whole genome shotgun sequence.
ACCESSION   DASSMB010000216 DASSMB010000000
VERSION     DASSMB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176202"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VFJ72_12850"
     CDS             complement(<1..961)
                     /locus_tag="VFJ72_12850"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HET7480392.1"
                     /translation="MYEQVYEPIANNLALSTIVAVIPIVVLFVLLAGVRMAAQWAALI
                     TLATALVIATVIYGMPFGTAISATLMGAAFGLFPIVWIVINAIFIYNVIVDTGNFNTV
                     RDSLAGVSNDRRIQVVLIAFVFGALLEATAGFGTPVAITASLMGGLGFQPLYAAALAL
                     LANTAPVAFGGFGIPILTGASVSGLDPMELSQMVGRQTPLLAVIIPGMLVTVMAGFRR
                     MLEVLPVVLVSGIAFAGTQLLVSNLLGPELADLLAALVTVVAVLGLLSFWRPASEWHF
                     EDEPPASQRESYDRPPLGRTLYAWSPFIIIVALFLIVQIPPVKA"
     gene            1266..2720
                     /locus_tag="VFJ72_12855"
     CDS             1266..2720
                     /locus_tag="VFJ72_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7480393.1"
                     /translation="MDRNRLIKTMEGILGPQSVISDHEQLRTYECDGLMNFRVRPTLV
                     VLPETPEQVQQAVRVCHDEGVPFVARGSGTGLSGGALPHEEGVLIVLARMREILDVDI
                     PNQRVIVEPGVINLKVTQEVAPEGYYFAPDPSSQLVCSIGGNVAENSGGAHCLKYGFT
                     TNHVMALEVVLPDGEMVHLGSKAPDPPGYDLLGAFVGSEGTLGIATRITLKVLRQPET
                     VQTLLAAFPSPDDAGAAVSDIIAAGTVPAAAEIMDSLAIEAAEAAVHPGYPDADSILI
                     VELDGPAAEVEHQFDEVVRICEENNSTEIRIAEDDAQRALFWKGRKAAFAAVGRISPD
                     YFVQDGVIPRTALPEVLRKIKEMSDQHGLRVANVFHAGDGNLHPLVLYNGNVEGEAER
                     AEELAGEILVLCVELGGSITGEHGVGEEKKHYMPKMFSEDDLNTMQLLRCAFDPHALS
                     NPEKVFPTPRLCGEVRPGPRRIHPVEKAGLAQVF"
BASE COUNT          548 a          895 c          961 g          412 t
ORIGIN      
        1 cggcctttac cggcggtatc tggacgatca ggaacagcgc cacgatgatg atgaacggcg
       61 accaggcgta gagcgtgcgt ccgagcggcg gcctgtcgta gctctcgcgc tgcgaagcgg
      121 ggggctcgtc ctcaaagtgc cactcgctgg cgggccgcca gaacgagagc aggccgagga
      181 cggcgaccac ggttacgaga gcggccagaa ggtccgcgag ctccgggccg aggaggttcg
      241 agacgaggag ctgcgtcccg gcgaaggcga tgccgctgac caggaccacc ggcaagacct
      301 ccagcatccg ccggaaaccg gccatcacgg tgacgagcat gccggggatt atgaccgcca
      361 ggagcggcgt ctgacggccg accatctgcg agagctccat cgggtcgaga ccggacacgg
      421 aggccccggt caggatcggg atgccgaacc cgccgaaggc gaccggcgcg gtgttcgcca
      481 gcagggccag cgccgcggcg tagagaggtt ggaagcccag gccgcccatc agggaggcgg
      541 tgatggcgac cggcgtcccg aagccggcgg tcgcttcgag gagcgcgccg aagacgaacg
      601 cgatcaggac gacctgtatc cgccggtcgt tgctcactcc cgccagcgag tcccggacgg
      661 tgttgaagtt gccggtatcc acgatgacgt tgtagataaa gatggcgttg atgacgatcc
      721 agacgatggg gaagaggccg aaagcggctc ccatgagcgt tgcgcttatg gcggtgccaa
      781 agggcatgcc gtagatcacg gtcgctatca cgagcgccgt cgccagggtt attagcgccg
      841 cccactgcgc cgccatgcgt accccggcca gcaatacgaa caacacgact atagggatga
      901 ccgcgactat cgtagagaga gccaggttgt tggctatcgg ctcgtagact tgctcgtaca
      961 tccgccccac ctccgtgtgt cgccgccgta tccagttgtc cgtgtcgctc gcgcagcgct
     1021 accagaccat gcgagaacca cttaacagca tggcacctca cgccatccgg cgcaagccgc
     1081 gccgggaaga cgaatccgtc ccgcgggccc cggctttcgg ttagaatgag atgaggaacc
     1141 ggggctggac ctcgcggggg tggaaggact tctatccggc taccgccagc gcgggacaga
     1201 tgagagagtt agcgaaagcg ggacgggaca acggggccgc ggggtctcaa gaaggagggt
     1261 catacatgga tcgcaatcgc ctcataaaga ctatggaggg gatcctgggt ccgcagagcg
     1321 tgatctcgga ccacgagcaa ctccgcactt acgagtgcga cggcctgatg aacttccgcg
     1381 tcagaccgac cctggtggtc ctccccgaga cacccgaaca ggtccaacag gcggtccggg
     1441 tctgtcacga cgagggagta cccttcgtgg cgcgaggctc cggcaccggc ctctccggcg
     1501 gggcgctacc gcacgaggaa ggagtcctca tagtgctggc gaggatgcgc gagatcctgg
     1561 acgtagacat cccgaaccag cgcgtaatcg tcgagcccgg cgtcatcaac ctgaaagtga
     1621 cccaggaagt cgccccggag ggctactact tcgctcccga cccctccagc cagctagtct
     1681 gctccatcgg cggcaacgta gccgagaact ccggcggcgc ccactgcctg aagtacggct
     1741 tcacgacgaa ccacgtcatg gcgctagaag tcgttctgcc cgatggcgag atggtgcatc
     1801 tggggagcaa ggctcccgac cctccgggct acgacctgct cggcgctttc gtcgggtcgg
     1861 agggcacgct ggggatagcc accaggatca cgctgaaggt actgcgccag ccggagaccg
     1921 tgcagacgct tctcgcggcg ttcccgagcc cggacgacgc tggggcggcg gtctcggaca
     1981 tcatcgcggc cgggaccgtg ccggcggcgg cggagatcat ggactccctg gcgatagagg
     2041 cggcggaggc ggcggtccat ccgggctacc cggacgcgga ctccatcctg atcgtggagc
     2101 tggacggtcc ggcggccgag gtcgagcatc agttcgacga ggtggtcagg atctgcgagg
     2161 agaacaactc gaccgagatc cggatcgccg aggacgacgc ccagcgggcg ctgttctgga
     2221 agggtcgcaa ggccgcgttc gcggcggtcg ggcgcatcag ccccgactac ttcgtgcagg
     2281 acggcgtgat cccgcgcacc gctctcccgg aagtgcttcg gaagatcaag gagatgagcg
     2341 accagcatgg cctgcgggtc gccaacgtct ttcacgccgg cgacggcaac ctgcacccgc
     2401 tcgtcctcta caacggcaac gtcgagggcg aggccgagcg cgccgaggaa ctggccgggg
     2461 agatactggt cctctgcgtc gagctcggcg gctccattac cggcgagcac ggcgtcggcg
     2521 aggagaagaa acactacatg cccaagatgt tcagcgagga cgacctcaac accatgcagc
     2581 tactacgctg cgcctttgac ccgcacgcgc tctccaaccc cgagaaagtc tttcccacgc
     2641 cgcgcctgtg tggagaggtc cggcccggcc cgcgccgcat acacccggtc gagaaagccg
     2701 gactggcgca ggtgttctag gtggagaccg ctacgcttgt agagaggatg cgggggatcc
     2761 tgggcgcgga aggcgttatc tccgagcacg agcaactccg cacctacgag tgcgac
//
LOCUS       DASSMB010000217         8554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1199366, whole genome shotgun sequence.
ACCESSION   DASSMB010000217 DASSMB010000000
VERSION     DASSMB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199366"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1692)
                     /locus_tag="VFJ72_12860"
     CDS             complement(28..1692)
                     /locus_tag="VFJ72_12860"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET7480394.1"
                     /translation="MVSVTDSGTKYIFVTGGVVSSIGKGTSAAALGMLLKSRGYRVVL
                     QKFDPYINVDPGTMNPYQHGEVYVTEDGAETDLDLGHYERFLDENLGRLSNVTTGSVY
                     WEVISRERRGDYLGATVQVIPHITNEIKNRIGRLGQDKDIVITEIGGTVGDIESLPFL
                     EAIRQFRNDVGRSNVLYMHVSYVPYIEAAGELKTKPTQHSAQRLREIGISPDVLICRA
                     DRPIGEDIRRKISLFTDTELDSVIPAENAPTLYDIPLRLHDEGLDDMILQKLGMSSPP
                     TDLAEWRHMVSRMHDAEETVRIAIIGKYIKLQDAYLSIVEALGHAGGAHGLRVELDWV
                     DSETLPDREATKRRLGEVDGVLVLPGFGHRGTEGKIEAARYARENGVPYLGLCLGMQI
                     EVIEFARNVAGLEDANSTEFDQETPHPVIDIMADQVGVDKGGTMRLGRYPCSIVPGTL
                     AAEVYDTDLVNERHRHRYEVNNGYRPVLAEAGMVFSGTSPDGRLVEIAELKDHPFYIG
                     SQFHPEFRSRPLRPHPLFKGFVGACGESLKKTSELEQAGIEERAAR"
     gene            complement(1676..2485)
                     /locus_tag="VFJ72_12865"
     CDS             complement(1676..2485)
                     /locus_tag="VFJ72_12865"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480395.1"
                     /translation="MVTALLLTPIGAAATLLALQLARTRLPHRENFTGNRIPTATGLT
                     LLPIILMMIMAGAAGWYAVGLADWGYLFYSLLAGVVGYLDDVWGGARVRGFRGHLGAL
                     ARGRVTTGAVKVFALGGGAMVLGALMFEVSVAAVFAGFLIAGCVNLANLLDLRPGRAI
                     KFLALPILALLFLAPGTAALTVLPILGGMAALFYFDLRGRIMLGDAGAAILGAVPGYL
                     VASCGPGIVWWTAALVILGLTVIAEVSSISRLIQEVEILRRLDLWGRGKRD"
     gene            complement(2479..3177)
                     /locus_tag="VFJ72_12870"
     CDS             complement(2479..3177)
                     /locus_tag="VFJ72_12870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7480396.1"
                     /translation="MSQRAVAVVPARDEADRIQGTIESLSGIPMLLRIIVVDDGSADA
                     TAKVAGAAGAQVLSSTPSGRPAGKGRALISGLRHARTLGPDAFLLADADLGPSAAGVA
                     SLLGCLSPKTPAAIAVFPRAAATGGGFGAVKSFSRHAIARRNTRGFVPAEPLSGQRAM
                     TAPALDALPGLAPGFGVEVGMTLDWLAADIVPVEVEIPLSHRPTGRSISGFAHRARQG
                     LDILRALHGTRLPW"
     gene            complement(3174..3995)
                     /locus_tag="VFJ72_12875"
     CDS             complement(3174..3995)
                     /locus_tag="VFJ72_12875"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HET7480397.1"
                     /translation="MPDLRYHLVSLISVFLALAIGILLGTAMADRGVISDRLQAQITD
                     VQDRFEAQQKDMARRDEEINRLRQKTTQEQDLAERMSQVVITNRLRNEKVALVTGPWA
                     DDDVVGAVENVLSQAGVEFTSNTQLDAPQTAEITSPETTGETTVQSDDIYRDQTREVL
                     RGSEDAGQPQFVVFVGGGMVPPDAPPGALRIVTSSERTMLESWRGSGLNVVGAESSTT
                     RRSEIELFQDIPIPSVDNADQPAGQAALILVIADDLEGSYGTKSTASDLFPPAAT"
     gene            complement(3999..5171)
                     /gene="steA"
                     /locus_tag="VFJ72_12880"
     CDS             complement(3999..5171)
                     /gene="steA"
                     /locus_tag="VFJ72_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HET7480398.1"
                     /translation="MSRLSRPSMTGAEDDFRLSGRAVLGRKTKDIVSRLNSDTIPVLN
                     HENLDRVTAEMLVESGVNAVVNAAGSSTGAYPNLGPFILARSGVYILDGVGQKVFERL
                     KTGDQIELRGDGVYRDNELVAAGEHLGLETAERRLRESRAVVGKALESFAQNTVEFMR
                     REQDLLFSRLEVPEEIAHEIRGRHVLIVVRGYDYRQDLAALGPYLRGVRPFVIGVDGG
                     ADAVLEAGHRVDLVFGDMDSVSPRALQAAGRVLVHAYPDGSAPGLQRVVEVGIKDADK
                     LPAPGLSEDIAILLAEQCGAELIVAVGTHVGLVEFLDKGRRGASSTFLTRLKVGARLV
                     DAKGVSKLYPTHLPLGPLVALVVAGLVAISAVVYSSEQIAFIFKLLAIKLRLLLGV"
     gene            complement(5168..6841)
                     /gene="recN"
                     /locus_tag="VFJ72_12885"
     CDS             complement(5168..6841)
                     /gene="recN"
                     /locus_tag="VFJ72_12885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7480399.1"
                     /translation="MLTEISVENVALIEGATLELEPGLNAITGETGAGKTLLATALQM
                     LLGGRARSEAVRAGAKRATVEGTFVLPDDIARRVLSEALGDLAEELAEDAGEGIPLRR
                     TLTADGRSRCYVGGVTVPVKALAALGERLVSYHGQREQTRLTEPAEQLRILDDFLDEG
                     SLRARDEHETLWHTVEKDRRELQEISASSEARLREADFLRFQVAEIEESGYTSQEVAE
                     LTRDRDRLRNITDLMEATAAATAALSSEESGAVVAAANAASELDRAARHDDGLSGLLE
                     RLNGVSAELEDVVYELRTYVDELEADPGRLDAVEERLGTLRGLERKYGPDVEAYLDEA
                     RERLTRLENSDAATLEIEDRIAEGERRLGELAAEVSAGRERAAVSLAEKVRENLADLN
                     LGSTVFKAELTQVSPGPLGLERVEYVIQPNPGEPELPVRRYASGGELSRIMLAIRLSQ
                     ERVEPGATYIFDEVDAGIGGETATAVGRKLRKLGERNQVLTITHLPQIASEAGSHVVV
                     AKEEAEGRTITRVRRVDGDERRKELARMLSGRVDEASLAHASEMLVGRR"
     gene            complement(6855..7664)
                     /locus_tag="VFJ72_12890"
     CDS             complement(6855..7664)
                     /locus_tag="VFJ72_12890"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET7480400.1"
                     /translation="MGEGAKLTEVRRVGIVSNPAVSGGYFERLVRTLEGRGVEVAQVV
                     ESPDGEAGREDVDLVFVLGGDGTMLRASRIFPKQVLLGVNLGRVGFMSGMLRDDIEGG
                     VAKVLDGGLHVQEYRMLEVRVEGEKHLAANDAVLIKQRPHQLVSIDVSVAGEELFAFR
                     CDGFIAATPLGSTAYALSAGGPIISGDAECYVLVPIAPHALVSRPLVLGEDQVAELRL
                     VERTALLSVDGGEPREIPAGGRVEIRLSRESVRIGRTDEWTWWQAVRRTFL"
     gene            complement(7657..8448)
                     /locus_tag="VFJ72_12895"
     CDS             complement(7657..8448)
                     /locus_tag="VFJ72_12895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET7480401.1"
                     /translation="MREAGQRLDLLLVERGLAESRSRAQGLIMAGAVRVDGEVITKAG
                     SRVPSDRTLAVDGENPYVSRAGEKLAGALDDFGVDLSGRECLDAGASTGGFTDVLLQR
                     GAARVISVDVGYGQLDWRLRKDPRVTVMERTNVRRLSGGDLPFEPDLLVADLSFISLA
                     VALENLLSSTPSIREAILLVKPQFEAGPEQVGRGGLVRDPEVRSAVIAGVAKSFAGLG
                     FGAVGVARAAVAGRKSGNQEYPLWLARGADVQLDEAGIREVVGDG"
BASE COUNT         1424 a         3034 c         2674 g         1422 t
ORIGIN      
        1 gggtagccga tgccgtcgcg gcggcgttca gcgggccgcc cgctcctcta tgccggcctg
       61 ctctagctca gaagtctttt tcaagctctc gccacaggcc ccgacgaagc ccttgaagag
      121 cgggtgcggg cgcaggggcc gactcctgaa ctccgggtgg aactggctcc cgatgtagaa
      181 aggatggtcc tttagctcgg ctatctccac gagccgtccg tcgggtgaag taccggagaa
      241 gaccataccg gcctcggcga gcacgggccg gtaaccattg ttcacctcat agcgatgccg
      301 gtggcgctcg ttgaccaggt ccgtatcgta gacctcggcg gcgagtgtgc cggggacgat
      361 gctgcacggg tagcgcccca ggcgcatcgt gccgccctta tccacgccga cctgatccgc
      421 catgatgtca ataaccggat gcggggtctc ctgatcgaac tccgtcgagt tggcgtcttc
      481 cagccccgcg acgttccggg cgaactctat gacctctatc tgcatgccca gacagagacc
      541 caggtacggc acgccgttct cccgcgcgta gcgggcggcc tcgatcttcc cctccgtgcc
      601 ccggtgcccg aagcccggca gcacgagtac gccgtccact tcgccgagac gccgtttggt
      661 cgcctcccgg tccgggagag tctccgagtc cacccagtcg agctcgaccc tgagcccgtg
      721 cgcgcctccg gcgtgaccca gcgcctcgac tatcgagagg taagcgtcct ggagcttgat
      781 gtacttgccg atgatggcga tcctgacggt ctcctcggcg tcgtgcatcc gggagaccat
      841 atgacgccac tcagcgaggt ccgtcggcgg cgaggacatc ccgagcttct ggaggatcat
      901 gtcgtccagc ccctcgtcgt gcagcctgag cggtatatcg tagagcgtcg gggcattctc
      961 ggcggggatc accgagtcga gctccgtgtc ggtgaagagc gatatcttcc tgcggatgtc
     1021 ctcacctata ggacggtcgg cccggcagat caggacatcc ggcgagatgc cgatctcacg
     1081 cagccgctgc gcggagtgct gggtcggttt cgtcttcagc tcacccgccg cctcgatgta
     1141 cggcacgtag gagacgtgca tgtacaggac gttgctccgg ccgacatcgt ttcggaactg
     1201 ccggatcgcc tcaaggaacg gcaggctctc gatgtcgcca accgtgcccc ctatctccgt
     1261 gatgacgatg tccttgtcct gcccgagacg cccgatgcgg ttctttatct cgttcgtgat
     1321 gtgcgggatg acctgcaccg tcgccccgag ataatccccg cgccgctccc ggcttattac
     1381 ctcccagtag acgctccccg tcgtcacgtt ggagagacgc cccaggttct cgtccaggaa
     1441 acgctcgtag tggccgaggt cgaggtcggt ctcggcgccg tcctcggtaa cgtatacctc
     1501 accgtgctgg taagggttca tggtgccggg gtctacgttg atgtaagggt caaacttctg
     1561 gaggacgacg cggtagcccc ggctcttgag gagcataccg agcgcggctg cgctggtgcc
     1621 cttgccgatg gacgatacga cgccgcccgt aacgaatatg tacttggtcc cggaatcagt
     1681 cacgcttacc acgcccccaa agatctaatc tccgtaatat ctctacctcc tgtatcaacc
     1741 tggagatgga cgacacttcc gcgatcacgg tcaatcccag tatgaccagc gccgccgtcc
     1801 accagactat ccccggcccg cacgaggcca cgaggtagcc cggaacggct cccaggatgg
     1861 ccgctcccgc atcaccgagc atgatacgcc cccgaaggtc aaaataaaat agcgcggcca
     1921 tgccgccgag gatcggcagc acggtcagag ccgccgtccc cggcgcgaga aagaggagcg
     1981 ccaggatcgg gagcgcgagg aacttgatcg cccgccccgg acgaaggtcc aggaggttcg
     2041 ccaggttgac gcatccggcg atcaggaatc ccgcaaatac cgccgccaca gagacctcga
     2101 acatcagcgc gcccaacacc atcgctccgc cccccagcgc gaataccttg accgcgcccg
     2161 tcgtcaccct tccacgagcc agcgccccga gatgcccccg gaagccccgc acccgcgccc
     2221 caccccacac gtcatccaga taccccacaa cccccgccaa cagcgagtag aagagatacc
     2281 cccagtcagc caggcccacc gcataccatc ccgcggcgcc agccataatc atcatgagta
     2341 ttatgggtaa taacgtcagg ccggtggcgg tcgggatgcg gtttccggtg aagttctcgc
     2401 ggtgtggcaa tctggtccgg gcgagttgca aggcgaggag ggttgccgcg gcgccgatag
     2461 gagttaggag gagggccgtc accacgggag ccgggttccg tggagggcgc ggaggatgtc
     2521 gagtccctgg cgggcgcggt gggcgaagcc ggagatgctg cggccggtcg gacggtggga
     2581 cagcggtatc tcaacctcga ccgggacgat atcggcggcc agccagtcca gggtcatacc
     2641 gacctcgaca ccgaaacccg gagccagtcc ggggagggcg tccagagcag gggccgtcat
     2701 cgcgcgctgt ccggagaggg gttcggcggg gacgaagcct cgggtgttgc gtcgagctat
     2761 ggcgtggcgg gagaagctct tgacggcgcc gaatcctccg ccggtcgcgg cggcgcgcgg
     2821 gaatacggct atggcggcag gtgttttcgg agagaggcat ccgaggaggg aggcgacgcc
     2881 agcggcggag ggtccgaggt ccgcgtcggc gaggaggaag gcgtcgggtc caagcgtgcg
     2941 ggcgtgtcgg aggcccgaga tcagggcccg gcctttcccg gccgggcggc cggacggcgt
     3001 agaggacagc acctgcgcgc ctgcggctcc cgcgaccttc gcggttgcgt ccgcgctgcc
     3061 gtcgtccacg acgatgatac gcaatagcat tggaatcccg gaaagggact cgatggtccc
     3121 ctggatacgg tccgcctcgt cgcgggcggg gactacggct acggccctct ggctcaagtc
     3181 gcggcgggtg ggaagaggtc cgacgcggtg gacttggtgc cgtaggagcc ctccagatca
     3241 tcggcgataa ccaggatcag ggccgcctgt cccgccggct ggtcagcgtt gtccaccgag
     3301 gggataggga tgtcctggaa aagctcgatc tcgctcctcc tggtagtaga ggactccgcg
     3361 ccgacgacgt tcagaccgga gcctcgccac gactcgagca tggtccgctc cgaggaggta
     3421 acaatccgca gcgcgcctgg gggagcatcg ggcgggacca tccccccgcc gacgaagacc
     3481 acgaactgcg gctgtcccgc gtcctccgaa cctcgcagga cctcgcgcgt ctggtcccgg
     3541 tagatatcgt cggactggac cgtcgtctcg ccggtcgtct ccggcgatgt gatctccgcc
     3601 gtttgcggag cgtcgagctg ggtgttggag gtaaactcga cccccgcctg tgagagcacg
     3661 ttctcgacgg cgcctaccac atcgtcatcg gcccagggtc cagttaccag cgccaccttc
     3721 tcgttccgga ggcggttcgt gattacgacc tgcgacatgc gctccgccag gtcctgctcc
     3781 tgggttgtct tctgccgcaa cctgtttatc tcctcgtcgc gccgcgccat gtccttctgc
     3841 tgggcctcga agcggtcctg aacgtcggta atctgagcct gcagccggtc actgatcaca
     3901 ccccggtcgg ccatcgcggt ccccaggagg atgccgatag cgagggccag gaacacggag
     3961 atcagggata cgaggtgata cctgagatcc ggcatgcgct agaccccgag caagagccgg
     4021 agcttgatgg cgaggagctt gaatatgaag gctatctgtt ccgaggagta gacgacggcc
     4081 gagattgcga cgagccccgc cacgacgagc gcgaccagcg gtcccagcgg caggtgcgtc
     4141 gggtagagct tgctcacacc cttcgcgtcc accaggcgcg cccctacttt caggcgggtc
     4201 aggaaggtag aggacgcccc cctgcgaccc ttgtccagaa actccacgag cccgacgtga
     4261 gtcccgacgg caacgatcaa ctcggccccg cactgctcgg cgagcaggat cgcgatatct
     4321 tccgagagcc cgggcgcggg gagcttatcc gcgtccttta tcccgacctc gacgaccctt
     4381 tgcagtcccg gagcggagcc gtccgggtag gcgtggacca gcacgcggcc ggcggcctgc
     4441 agcgcccggg gcgagacgga gtccatgtcg ccgaagacca ggtccacgcg gtgtcccgcc
     4501 tccagcaccg cgtccgcgcc gccgtcaacc ccgataacaa acggccgcac gccccgcagg
     4561 taggggccga gagcggcgag gtcctgccgg tagtcgtagc cccgcaccac tattagcacg
     4621 tgacggcccc ggatctcgtg cgcgatctcc tccggcacct cgagccggga gaacagcagg
     4681 tcctgctccc gccgcatgaa ctcgacggtg ttctgggcga agctctcaag agccttcccc
     4741 acgacggccc ggctctcccg caggcgccgc tcggccgtct caaggccgag gtgctccccg
     4801 gcggccacca actcgttgtc ccggtagacg ccgtcgccgc gaagctcgat ctgatccccc
     4861 gtctttagcc gctcgaagac tttctggccg acgccgtcga ggatgtacac cccggacctg
     4921 gcgaggatga acgggccgag gttcggatag gcgcccgtcg agcttccagc agcgttcacg
     4981 acggcgttga ccccggactc gaccagcatc tccgccgtca cccggtccag gttctcgtgg
     5041 ttcagtacgg ggatggtgtc ggagttcaac cgggacacga tgtccttggt cttcctgccg
     5101 agcacggctc tacccgacag gcggaaatcg tcctcggcac ccgtcatgct cgggcgcgag
     5161 agccggctca ccgtcgcccc accagcatct cggaggcgtg ggccaacgac gcttcgtcca
     5221 cccgtccgga gagcatccgc gccagctcct tgcgccgctc gtctccatcg acgcgccgga
     5281 cgcgcgtgat agttcgcccc tcggcctcct ccttcgcgac cacgacgtga gagcccgcct
     5341 ccgaggctat ctgggggagg tgcgtgatcg tcagtacctg gttgcgctcg cccagcttcc
     5401 ggagcttcct gcccacggcg gtcgccgtct cgccgccgat accggcgtcc acctcgtcga
     5461 agatgtaggt agctcctggc tccacccgct cctgcgagag ccggatcgcc agcatgatcc
     5521 tggagagctc gcctccggag gcgtagcgcc gcaccggcaa ctctggctcg ccggggttcg
     5581 gctggatgac gtattcaacc cgctccaacc ccaacggtcc aggagagacc tgcgtaagct
     5641 ccgccttgaa gaccgtgcta cccaggttca ggtccgccag gttctcccgc accttctccg
     5701 cgagactcac cgcggcccgc tccctgcccg ccgaaacctc cgccgccagc tcaccgagcc
     5761 gccgctcgcc ctcggctatc cgatcctcta tctccaaagt cgcggcatcg gagttctcca
     5821 gccgggtcag gcgctctcgc gcctcatcca ggtaggcctc cacgtcgggg ccgtacttgc
     5881 gttccagacc cctcaaggtc ccgagacgct cctctacggc gtccagccgg ccggggtcgg
     5941 cctccagctc atccacgtag gtccgtagtt cgtagaccac gtcttcgagt tccgccgaga
     6001 caccgttaag gcgctctaga agaccggaga gcccgtcgtc gtggcgggcc gcgcggtcca
     6061 gctcggaggc ggcgttcgcg gctgccacga cagcgccgct ctcctccgac gagagcgcgg
     6121 cggtcgcggc ggcggtcgcc tccatcaggt ccgtgatgtt acgcaggcgg tcgcggtcgc
     6181 gggtcagctc ggcaacctcc tgcgaggtgt agccggactc ctcgatctcc gcgacctgga
     6241 agcgaaggaa gtccgcctcg cgcaaccggg cttcggagga ggcggagatc tcctggagct
     6301 ccctccggtc tttctctacg gtgtgccaga gcgtttcgtg ttcatccctg gcccgcaggg
     6361 agccttcgtc caggaagtcg tccaggatgc gaagctgttc ggccggctct gtgaggcgcg
     6421 tctgctcccg ttggccgtgg tacgagacta gccgctcgcc aagagccgcc agcgccttca
     6481 ccgggaccgt cacgccgccg acgtaacacc gcgaccggcc atcagcggtg agcgtgcggc
     6541 gcagcggtat gccttcgccc gcgtcctcgg ccagttcctc ggccaggtct cccagcgcct
     6601 ccgagagcac gcgacgagcg atatcgtcgg ggaggacgaa ggtgccttcg acggtggccc
     6661 gtttcgcccc ggcccggacg gcttccgagc gggcgcgtcc gccgaggagc atctgcagag
     6721 cagttgcgag cagcgtctta ccggcgcccg tctcgccggt gatcgcgttg agaccgggtt
     6781 ccagttcaag agtcgcccct tcgatcaggg caacgttctc tacggagatc tccgtgagca
     6841 atccaaatct cccgttatag gaaagtcctt cgcacggcct gccaccacgt ccactcgtcg
     6901 gtccggccta ttctaacgct ttcccgggag agcctgatct caaccctccc gccggcgggg
     6961 atctcgcgcg gctccccccc atccaccgat aacagagcgg tccgctcgac cagcctcagt
     7021 tccgctacct gatcctcccc gagaacaagc ggccggctga cgagagcgtg cggcgctatc
     7081 gggaccagca cgtaacactc ggcgtcgccg gagatgatcg ggcctccggc cgacagcgca
     7141 taggccgtgg aacccagcgg ggtggccgcg atgaagccgt cgcaccggaa cgcgaagagc
     7201 tcctctcccg ccaccgagac gtctatcgag acgagctggt gggggcgctg cttgatgagc
     7261 acggcgtcgt tggccgccag gtgcttctcc ccctcgacgc ggacctccag catccggtac
     7321 tcctggacgt gcaggccccc gtcaaggacc ttcgccacgc cgccttctat gtcgtccctg
     7381 agcatcccgc tcatgaagcc gaccctgccc aggttcacgc ccagcaggac ctgtttcgga
     7441 aagatcctgg acgcccggag catcgtcccg tcgcccccca gcacgaagac caggtccacg
     7501 tcctcgcggc ccgcctcgcc gtccggcgac tcgacgacct gcgcgacctc gacgcccctg
     7561 ccctccagcg tccgcacgag ccgctcgaag tacccgccgc tcaccgccgg gttcgacacg
     7621 atcccgacgc ggcgtacctc ggtaagcttc gccccctcac ccatccccaa ccacctcccg
     7681 gatacctgcc tcgtcgagtt gcacgtctgc gccccgcgcc agccacagcg ggtactcctg
     7741 gttgccggac ttccgtcccg caaccgccgc cctggcgacc ccaacggcac cgaaccccag
     7801 gccggcgaat gacttcgcaa cacccgcgat gacggcggag cgcacctcgg ggtcgcgcac
     7861 gaggcccccg cgcccgacct gctccggccc ggcctcgaac tggggcttca cgagcaggat
     7921 agcctccctt atggacggcg tgctggagag caaattttcc agcgccacgg ccagcgatat
     7981 aaacgaaagg tccgccacca gcaaatccgg ctcaaaaggc agatccccgc ccgaaagccg
     8041 ccgcacgttg gtccgctcca tcactgttac gcgcgggtct ttacgcagcc tccagtctag
     8101 ctgaccgtag ccgacatcca ccgagatcac gcgcgcggct cccctttgaa ggaggacgtc
     8161 cgtgaagccc ccggtagacg ctccggcgtc caggcactcg cgtccagaaa ggtccacgcc
     8221 aaaatcatcc agggcgccgg caagcttctc tccggcgcgg gagacatagg gattctctcc
     8281 gtccactgcg agggtcctgt ctgacggcac gcgggatccg gccttggtta tcacctcgcc
     8341 gtccacgcga acggctcccg ccattatgag cccctgggcg cggctgcggc tctccgccag
     8401 gccgcgttcc actagcagga gatccaggcg ctgtcccgct tctctcatcg gacggagcag
     8461 actctagctg ccccggtggc gcacgaagag cgccatctcc cgcagccccg acgtgtcgcc
     8521 gtccacccgc gagagcgctt ccagggcgcg tttg
//
LOCUS       DASSMB010000218        11395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_694326, whole genome shotgun sequence.
ACCESSION   DASSMB010000218 DASSMB010000000
VERSION     DASSMB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694326"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFJ72_12900"
     CDS             complement(<1..608)
                     /locus_tag="VFJ72_12900"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET7480402.1"
                     /translation="MLGSFLVMLREGFEASLLVAIVLAYLTQTGRKEDARGVWYGVGA
                     AVLISIVTAGVLFVTANGLEGEAGIYFKSGAMWLAVAFLTYMVLWMRRQARTMAQGIR
                     RDVDSAVERGGLLALGMLVFVMVLREGIETGLFMFGITQNATPLGVGIGAVAGTAAAV
                     GLGYAVYIGGKRINLGAFFKITGILLIVVAAGLLARGVAIME"
     gene            763..1719
                     /locus_tag="VFJ72_12905"
     CDS             763..1719
                     /locus_tag="VFJ72_12905"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7480403.1"
                     /translation="MNRVLVTGAAGFIGSHLVDRLLADGHEVIGLDAFTANYSRRSKE
                     RNLAGAIGEDGFRLVDGDLLTENLDALLQDVDGIAHLAGEPGVRRSWGDNFSRYLQRN
                     VLATQRLLEAAGRSAVAGFVHASSSSVYGSDRGGPVSEGHVREPASPYGLSKLAAEEL
                     VGVYAREQGVPATVLRYFTVYGPRQRPEMALSRFIFAANRGRPVEVFGDGEQVRDLTY
                     VSDVVDATLAALKTGPGGVYNVGGGARVTVNGMLDAVRRVTGSAVPVSYGPAACGDVV
                     STWADASRAARDLGYRPSVTLDEGVAAQVEWAIREMPAAGMV"
     gene            1727..3745
                     /locus_tag="VFJ72_12910"
     CDS             1727..3745
                     /locus_tag="VFJ72_12910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7480404.1"
                     /translation="MQAAILGISLYLPGHFLARRLARKGDGLAELILLRVAVSLALAG
                     PALVALALAGWFKTPVIAGCLAVISVGAFFIGRGGEGQGRRSGWDIGALGLVVGSFAL
                     YARPAEYVINSRDPGVYTLFAEKLARTGALISRDPLVGAVAPFHAFVEAKKYPGFYIL
                     GRDWIVPQFFPGPFAWLGLGDLAGGTWGLLYVVPVMGALAVGMAFALGSQLFERWAGL
                     LGAALLAASYTQVWWARHPSSEVMTQLLLLAGLWMAARFAREPDRMTGVLAGLLLGGA
                     MLVRVDAFLAALALPLLFGYDLLTRGDARRWLWPGVPLAVSATLALLYLNTLGGRYLY
                     TIYSQHGLDRVVAVAGYLIAAAILAVVALFVVRAKWGERTGRWVTARGGGLALVVALI
                     VAGVVLWAYFVLPVPWDSLPDGSRDYNAYRTQILLRLTWFTTPAVALLGLIGFVLAAS
                     RPSAGRIIVLGAFLSFGVLYTVVPNVSPDLPWATRRFVPVVFPVLCLLAGHAVAELGR
                     FLGHAGYRRVGFVAAGALTGLALGWTVYTALPVIRFQELQGAVRAFDHIERKVPESDV
                     VFMEMPDGYDITASTFEYAYGHPLLPYDHDRFRDEVDELAKAGLLKNAVYITVDGGPA
                     PLVSDVDFREVADAHLTLPRLAAVEKELPTKTETLRLDYRIFRVENEK"
     gene            3759..5201
                     /locus_tag="VFJ72_12915"
     CDS             3759..5201
                     /locus_tag="VFJ72_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7480405.1"
                     /translation="MRPASGFEILAARCWNVLNEGKSFHPVFVLGVFLLLHVADLGGA
                     EFWGRALPALALTAPLVLLFVRYDFPLRLRWSLWVFLGAFVAVFRFVDLGALALALGL
                     YVFFTVFFWGTFYYHLRTGAPRTNFIRFWRLVLENPDSTSGNFLEQVPKAFIVLLTLE
                     YLNAAPISTERAVLVLAFTGALAVASYLIHRRFFTWKPVYPSEPTADTNRGAPLAKRV
                     IVIVIDGCRLDRFHEAEKPYLERMMAGGTVFESVETTYPARTVVCFSSMFTGAAPERH
                     GITSNLVLKLGLRVESVFDSLRRAGKKGRLVGIAHLIDAFGDDVASVTSVAHNDKIDQ
                     NLIAAARRELTDHDPELLVLQLLAVDQNGHTRGTYYPEYVERIEITDALIEEFMGWCE
                     ERGYLEDAAVVLMADHGQGIGIGAHGHLSEGERFVPFAMWGSGVAAGKTVSEPRSILD
                     LAPTICYLLGVEPPSGSTGRTLIEALERRP"
     gene            5198..5881
                     /locus_tag="VFJ72_12920"
     CDS             5198..5881
                     /locus_tag="VFJ72_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7480406.1"
                     /translation="MSLLAVIVPAHNEEATIVELLERISAVRVPGRELRVFVIDDGST
                     DGTALLAGERGATVVSHPVNRGLGAALRTGLSVAVEVGAGAVVYLDADLEYFPEDIPA
                     VVAPVLDGRAEYVLGSRFMGGVRGMRAHRWIGNHVFTALLCLLTGRWMTDGQTGMRAF
                     SRKAARAAEIVHDYNYAQVLTLDLLDKGFSMREVPIQYRVREHGKSFINWRYPAKVLP
                     AIWRELRSP"
     gene            6018..6950
                     /locus_tag="VFJ72_12925"
     CDS             6018..6950
                     /locus_tag="VFJ72_12925"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7480407.1"
                     /translation="MGLDPQAEALLREMEAQGLPPFDEMSVPQARDVILAFRDLEGDP
                     EYVADVRDFTVPGPAGELPVRFYRPEVSGHVPLIVYFHGGGWVIGNVEVADKPCRSLA
                     NASGCAVASVEYRLAPESKFPAAPEDCYAATRWMAEHAEELGVDAGKIAVAGDSAGGN
                     LAAAVALMARDRGGPDLAYQLLIYPAVEAEADFPSVHENAEGYLLTSGAMKWFWGHYL
                     TGTADAENPYASPLRAEDLSGLPPALVVTCEFDPLRDEGEAYAERLRDAGVEVQATRY
                     DGMIHGFFWMAGVMDQTRPLMEEVGGALRSALLA"
     gene            complement(6954..8000)
                     /locus_tag="VFJ72_12930"
     CDS             complement(6954..8000)
                     /locus_tag="VFJ72_12930"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HET7480408.1"
                     /translation="MVKVAVIGGDGIGPEVTDAGVRVMRSAAESDPSLDLEFTEYPWG
                     CEYYLEHGRMMPEDALDTLAGFDTIYLGAVGWPTVPDHVSLWGLLLPIRRGFDQYVNL
                     RPARLLAGVKSPLAKPGELDITVVRENTEGEYSDSGGRIYRGTEHEIAVQESIFTRRG
                     VERVLRYAYETAKERQGNLVGATKSNGISITMPFFDEIFREVGEEYPDVRASLMHADA
                     LAARLVLSPQSFDVVVGSNLFGDLLSEITAAISGAIGIAPSANLNPEGRYPSLFEPIH
                     GSAPDIAGQGVANPSGAIWAASMLLENTGHPEAGQRILEALEATLQSGVKTRDLGGEA
                     STGEVTDAVTGRLR"
     gene            8170..8457
                     /locus_tag="VFJ72_12935"
     CDS             8170..8457
                     /locus_tag="VFJ72_12935"
                     /inference="COORDINATES: protein motif:HMM:NF016162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET7480409.1"
                     /translation="MPKTTLNGVTLAESGSTEVVEGNHYFPPDSVNRDHLEESATRTT
                     CPWKGEASYYSLRVDGETVEDAAWYYPEPKEAAENIRDHVAFYADKVRIED"
     gene            8557..8892
                     /locus_tag="VFJ72_12940"
     CDS             8557..8892
                     /locus_tag="VFJ72_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480410.1"
                     /translation="MDERNLDRANKAARQMAEISRESYRAVVERAFAARESNERLTRS
                     FFEEGLELFQDHAELNRRTLNRMAEQAQEHRDALLSLSRDSLTSYDDFVGSLSGFYDE
                     VTGEREREE"
     gene            9008..10417
                     /locus_tag="VFJ72_12945"
     CDS             9008..10417
                     /locus_tag="VFJ72_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HET7480411.1"
                     /translation="MKYDQSAQAAAGQPRADGSFKVNPLAAGVLRRNGLKSWTKPAPS
                     LYPHQWSWDSAFIALGLAHVDNRRATQELETLFASQWSNGKVPHIVFNPEAPPRSYFP
                     DAERWNSSALSEHAPSSPHTSGLCQPPVHAIAVWRIWETSQGKEGRRVEASLRFLREN
                     YPRLLSWHRYLMTARDPEDSGLVTIYHPWESGTDNSPRWDSALREVRVGDLPPYVRAD
                     LQHVSDPSHRPTDSEYDRFLWLLELLKRARYEEADIYRSHPFLVKDVLFSAILVAANE
                     ALLQIAEVVDAPGEDLSLIRAWIERGRHGIEASWDPVLNLSLDHDLRRGEPARVRTVA
                     GFAPLISGGLAPDRLQAQLRTLDSPHFAGNPDLERPLPPSTSPTEPGFHPRSYWRGPT
                     WPVANWLLWWSLLRAGERVRAAALRAEILAQLAHGGFAEYFEPFTGEPLGSREQSWTA
                     AVALDLIAAGDDAGNGTGG"
     gene            10470..>11395
                     /locus_tag="VFJ72_12950"
     CDS             10470..>11395
                     /locus_tag="VFJ72_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480412.1"
                     /translation="MSAPAGLYELVERFEGNIRAYKSGDYNETQARGDFIDPLMGLLG
                     WDVHNRQGKAPAYRDVVEEDRVKVGGGTKAPDYGFYAGGSRRFFLEAKKPSVDIAGDV
                     NPAVQLRRYAWSARLPLSILTDFEEFAVYDCRFEPIQDDRPATARLQLLTYDQYPEKW
                     DDIYSLFSGEAVLGGTLEKYAEENRKRRGTETVDAAFLREIEGWRSDLARDLAAHNNL
                     NSRGLNFAVQMTIDRIIFLRMAEDRGIEDYGRLLALVNGGDVYGRLLEVFRDADDRYN
                     SGLFHFRAERGRDEEPDTLTPHLKVGDATLKG"
BASE COUNT         1828 a         3766 c         3845 g         1956 t
ORIGIN      
        1 atctccatga tcgccacccc gcgcgccagc agaccggccg ccaccacgat gagcaggatg
       61 cccgtgatct tgaagaacgc gccgaggttt atccgcttgc ccccgatgta tacagcgtag
      121 cccaggccga cagccgcggc cgtaccagcc accgcgccta tcccaacgcc gagaggcgtc
      181 gcgttctggg taatgccgaa catgaacaat ccggtctcta taccctcgcg caagaccatg
      241 acgaagacga gcatccccag cgccagaagg ccgccgcgct cgaccgcgct atccacgtcg
      301 cggcgaatcc cctgggccat cgtgcgagct tgccggcgca tccagaggac catgtaggtg
      361 aggaaggcta ctgcaagcca catggctcca ctcttgaagt agattccagc ctctccctca
      421 aggccgttcg cggtcacgaa caagacccct gcggtaacga tagagatcag gaccgccgcg
      481 ccgaccccgt accaaacccc gcgggcgtcc tccttgcgtc cggtctgcgt cagatacgcc
      541 agcactatgg cgaccagcag agaagcctcg aacccctctc gcaacatcac caggaaagat
      601 cccaacaccc tcgttacacc tcccgccact tcggcgcttg acactaatga caatcatttg
      661 caataacttc accgtcaggg taatgtaccg ggcggtagtt cgtcaagtcg cggtcgggag
      721 ttttgtgaac tggatcaaaa cgtgggtcga aaagtggtcc ctatgaaccg ggtgctggta
      781 acgggtgcgg cggggtttat cgggtcgcac ctggtggacc ggcttctggc ggacgggcac
      841 gaggtcatag ggctggacgc cttcactgcc aactactccc ggcggagcaa ggagcgtaac
      901 ctcgccggag cgatagggga agacgggttc cggctggtgg acggggacct cctgaccgag
      961 aacctggacg cgctcttaca agacgtggat gggatcgcgc atctcgccgg cgagcctggt
     1021 gtgcgccgga gctggggaga caacttctcc cggtatcttc agcggaacgt gctcgccacc
     1081 caacggcttc tcgaagccgc cgggaggagc gccgtcgcgg gtttcgtcca cgcctcttcg
     1141 tcctcggtgt acggttcgga ccggggcggg cccgtgtccg agggacatgt ccgcgaaccg
     1201 gcctcgccgt acggtctctc caagttggcc gcagaagagt tggtaggcgt ttacgcgcgg
     1261 gagcagggtg tgccggcgac ggtgttgcgg tacttcaccg tctatggacc tcgccagcgg
     1321 ccggagatgg cgctctcgcg gttcatcttc gcggcgaacc ggggccgtcc ggtggaggtc
     1381 tttggcgatg gggagcaggt ccgagacctc acctatgtct ccgacgtcgt ggatgccacg
     1441 ctcgccgcct tgaagacagg gccgggcggc gtctacaacg tcggcggcgg ggcgcgggta
     1501 acggtgaacg ggatgctgga tgccgtgcgc cgggtcacgg gtagcgcggt gccggtatct
     1561 tatgggccgg cggcctgtgg ggacgtggtt tcgacctggg ctgacgcgag ccgggcggcg
     1621 cgggacctcg gctatcggcc gagcgtgacg ctcgatgagg gggtggcggc tcaggtcgag
     1681 tgggcgatcc gggagatgcc cgccgccggc atggtctgag aggataatgc aggctgcgat
     1741 actggggatc tcgctgtacc ttcccggtca cttcctggcg aggcggctcg cgcgcaaggg
     1801 tgacggcctg gccgagttga tcctgctccg ggtagcggtg agcctggcgc tggcaggtcc
     1861 ggcgctggtc gcactggcgc ttgccgggtg gttcaagacg ccggtgatcg cgggctgtct
     1921 ggcagtgatc tccgtcggag ctttttttat agggcgtggc ggagaaggac agggacgccg
     1981 gtcggggtgg gatatcgggg cgctggggct ggtggtgggg agcttcgcgc tgtatgcgcg
     2041 cccggcggag tacgtgatca acagcagaga ccccggcgtg tacacactct tcgcggagaa
     2101 gctcgcccgg accggcgctc tcatctcgcg cgaccccctt gtcggcgccg tcgcgccgtt
     2161 ccacgccttc gtcgaggcga agaagtaccc cggcttctac atcctggggc gggactggat
     2221 cgtcccgcag ttcttccccg gtcccttcgc ctggctcggc ctgggagacc tcgccggggg
     2281 gacctggggt ctactctacg tcgtccccgt gatgggcgcc ctcgcggtcg ggatggcctt
     2341 cgctctcggc tcccagctct tcgagcggtg ggccggtctg ctcggcgcgg cgctcctcgc
     2401 cgccagctac acgcaggtct ggtgggcgcg tcatccctcc agcgaggtca tgacccagct
     2461 actcttgctg gccggtctgt ggatggccgc gcgcttcgcg cgcgagccgg accggatgac
     2521 tggagtgctg gcgggcctct tgctcggtgg cgccatgctc gtgcgggtcg acgcctttct
     2581 agctgccctg gccctgccgc tgctcttcgg atacgacctc ctgactcgcg gcgacgcccg
     2641 gcgctggctc tggcccggcg tgccgctcgc ggtctccgcc accctcgctc tcctgtacct
     2701 gaatacgctc ggcgggcgct acctgtacac catctactcc cagcacggcc tggaccgcgt
     2761 cgtcgcggtc gcgggctacc ttattgccgc cgcgatcctg gccgtcgtgg ccctctttgt
     2821 cgtgcgggcg aagtggggag agcgtaccgg acgctgggtg acggcgcggg gcggcgggct
     2881 ggcgctcgtg gtcgcgctca ttgtggcagg agtcgtcctg tgggcctact ttgtgctgcc
     2941 ggtgccgtgg gacagcttgc cggacgggtc cagggactat aacgcttacc ggacccagat
     3001 cctgctccgc ctcacctggt tcaccacgcc ggccgtcgcc ttgctcggcc tgatcggttt
     3061 cgtcctcgcc gcctcgcggc cgagcgccgg acggatcata gtgctggggg cgttcctgag
     3121 cttcggggtt ctgtacacgg tcgtgccgaa cgtctcgccg gacctcccgt gggccacccg
     3181 acgctttgta cccgtagtct tcccggtact ctgcctgctg gccggacacg cggtggcgga
     3241 gcttggccgg ttcctcggac acgcagggta tcggcgggtg gggttcgtcg ccgccggagc
     3301 gctcaccggg ctggcgctcg gctggacggt gtacacggcg ctgcccgtca tccggttcca
     3361 ggagttgcag ggagccgtcc gagcgttcga ccatatagag cggaaggtgc cggagtctga
     3421 cgtggtgttc atggagatgc ccgacggtta tgacatcacg gcctccacct tcgagtacgc
     3481 ctacggacac ccgcttctgc cctacgatca cgaccgcttc cgggacgagg tggacgaact
     3541 cgcgaaggcc ggtctgctga agaatgccgt ctacatcacc gtggacggcg gacccgcgcc
     3601 gctcgtctcc gacgtggact tccgcgaggt cgccgatgct caccttacgc tgccccggct
     3661 ggcggcggtc gagaaggagc ttcccacgaa gaccgagaca ctgcggctcg actaccggat
     3721 cttccgggtg gagaacgaga aatgagagag gagaccccat gagaccggcc tccgggttcg
     3781 agatacttgc cgcccgctgc tggaacgtcc tgaacgaggg caagtccttc cacccggtgt
     3841 tcgtactggg cgtcttcctg ctgctgcacg tcgcggacct cggcggggcg gagttctggg
     3901 gccgggcgct acccgctctc gccctgaccg ctccgctggt gctgctcttc gtccgctacg
     3961 acttcccgct gcggctgcgg tggtcgttgt gggtcttcct gggcgcgttc gtggccgtgt
     4021 tccggttcgt ggacctcggg gcgctcgcgc tggctctcgg actgtacgtt ttctttacgg
     4081 tgttcttctg ggggacgttc tactatcacc tgcggaccgg ggcgccgagg accaacttca
     4141 tccggttctg gcggctcgtg ctggaaaacc cggactcgac gagcgggaac ttcctggagc
     4201 aggtcccgaa ggcgtttatc gtgttgctca cgcttgagta tctgaacgcc gctcctatta
     4261 gtactgaacg tgccgttctg gtgcttgcct ttaccggcgc tctggcagtc gccagctacc
     4321 tgatccaccg caggttcttc acctggaaac ccgtctatcc ctccgagccc acggcggata
     4381 ccaaccgcgg cgctccactc gcgaagcgcg tgatcgtcat cgtcatagac ggctgccgcc
     4441 tggaccgctt ccacgaggcg gagaagccct acctggagag gatgatggct ggcggcacgg
     4501 tcttcgagag cgtggagacg acctacccgg cccgcacggt ggtgtgtttc tcctccatgt
     4561 tcaccggcgc ggccccggag cggcacggca tcacgtccaa cctggtgctc aagctcggcc
     4621 tgcgggtcga gagcgtcttc gattcgttgc ggcgggccgg gaagaagggg cggctcgtgg
     4681 ggatagcgca cctcatagac gctttcgggg atgacgtggc gagcgttacc agcgtggcgc
     4741 acaacgacaa gatagaccag aacctcatcg ccgccgcccg ccgcgagctg acggaccacg
     4801 acccggagct tctggtgctg caactgctcg ccgttgacca gaacgggcac actcgcggca
     4861 cgtactaccc ggagtatgtc gagcggatag agatcacgga cgccctgatc gaggagttca
     4921 tgggctggtg cgaagagcgc ggctacctgg aagacgccgc tgtcgtcctg atggcggacc
     4981 acggtcaggg catcggcatc ggagcgcacg ggcatctctc cgagggagag cggttcgtgc
     5041 cgttcgcaat gtgggggagc ggcgtcgcgg cgggcaagac cgtcagcgag ccccgctcca
     5101 tcctcgacct cgccccgacg atctgctacc tgctcggcgt cgagccgccg tccggctcga
     5161 ccggccgtac cctcatcgaa gcgctggagc gccgcccgtg agcctgctgg ccgttatcgt
     5221 cccggcccac aacgaggagg cgaccatcgt ggagcttctc gaaaggatct ccgctgttcg
     5281 ggtgcccggc cgcgagttgc gcgtgttcgt gatcgacgac ggctcgaccg acggcaccgc
     5341 gctgctggct ggtgagcggg gcgccacggt cgtcagccat cccgtgaacc ggggtctggg
     5401 ggcggccttg cgcacggggc tctcggtagc ggtggaggtg ggcgcgggtg ctgtcgtcta
     5461 cctggacgcg gacctggaat acttcccgga ggacatcccc gccgtggtcg cgccggtgct
     5521 ggacggccgt gcggagtacg tgctcggcag ccgctttatg ggcggcgtgc gggggatgag
     5581 ggcgcaccgc tggatcggca accacgtctt caccgcgctc ctgtgtctgc tcaccggacg
     5641 atggatgacg gacggccaga cggggatgcg cgctttctcc cggaaagcgg cgcgcgcggc
     5701 ggagatcgta cacgactaca actacgccca ggtcctcacc ctggacctgc tcgacaaggg
     5761 cttctccatg cgggaggtcc cgatccagta ccgcgtccgg gagcacggca aatctttcat
     5821 caactggcgc taccccgcga aggtgctgcc cgctatctgg cgggagctac ggagcccgta
     5881 gcgcgccttc aaccacaggt tccttcgttc ctcttgacgg cgttcgcggc ggtgtgcaac
     5941 agtacggtcg ttggggatag gagtagcagc gttggccgcc gcgtcgaggt gggccgcgtt
     6001 tagccaggag gtgcgttatg ggtctggacc cgcaggcgga agcattgctc agggagatgg
     6061 aagcacaggg gctgccgccg ttcgatgaga tgagcgtgcc ccaggcgagg gacgtcatat
     6121 tggcattccg cgaccttgag ggcgatcccg agtacgtggc ggacgtacgg gacttcaccg
     6181 ttcccggacc ggcgggcgag ttgccggtcc ggttctacag gccggaggtt tcgggccacg
     6241 tgcccctgat cgtctacttc cacggcgggg gctgggtcat cggcaacgtc gaggttgcgg
     6301 acaagccctg ccgttcgctg gcgaacgcct ccgggtgcgc ggttgcctcc gtcgagtacc
     6361 gactggcccc ggagagcaag ttccccgccg ctccggagga ctgctacgcc gccacccgct
     6421 ggatggccga gcacgccgag gagcttggcg tggatgcggg gaagatcgcg gtggccggag
     6481 acagcgccgg gggcaacctt gccgccgccg tggcgctcat ggcccgggac cggggcggcc
     6541 cggacctggc ttaccagcta ctgatctacc cggccgtcga ggcagaggcc gatttccctt
     6601 ccgtacacga gaacgccgag ggatacctgc tgacctctgg agcgatgaag tggttctggg
     6661 gtcactacct gaccgggacc gccgacgcag agaaccccta cgccagcccg ctgcgcgccg
     6721 aagacctgtc ggggctgccg cccgcgctcg tcgttacctg cgagttcgac ccgctgcgcg
     6781 acgagggtga ggcgtatgcg gagcgcctgc gggatgcggg cgtcgaagtg caggccaccc
     6841 gctacgatgg catgatccac ggtttcttct ggatggccgg ggtcatggac cagacccggc
     6901 cgctcatgga ggaggtggga ggcgcgctgc ggtccgccct gctggcgtag cgcttaccgc
     6961 aacctgcctg taaccgcatc cgttacctcc ccggtgctcg cttctccgcc gaggtcgcgg
     7021 gtcttcacgc cgctctgcag cgtcgcttcc agcgcctcca ggatacgctg gcccgcttcg
     7081 gggtggccgg tattctccag cagcatggag gcggcccaga tcgcgccgga cgggttcgcg
     7141 actccctgcc cggcgatgtc cggagcggag ccgtggatcg gctcgaagag ggaaggatag
     7201 cgtccttcgg ggttgaggtt ggccgagggc gcgatgccga tggcgccgga gatggcggct
     7261 gttatctccg agagcaggtc tccgaagagg ttggagccga cgaccacgtc gaagctctgc
     7321 ggcgagagga cgagccgggc ggcgagggcg tcggcgtgca tcagggaggc ccgcacgtcc
     7381 gggtactcct cgccgacctc gcggaagatc tcatcgaaga acggcatggt gatgctgatg
     7441 ccgttggact tcgtcgcccc gacaaggttc ccctggcgct ctttcgccgt ctcgtaggcg
     7501 tagcgtagta cgcgctcgac accccgacgg gtgaagatgc tctcctgcac cgcgatctcg
     7561 tgctccgtgc cccggtagat gcgtccgcct gagtccgaat actcgccctc ggtattctcc
     7621 cgcacgacag taatgtcaag ctctccgggt ttcgccagcg ggctctttac gcccgccagc
     7681 agccgtgcgg gccgcaggtt cacatactgg tcgaagccgc gccggatcgg cagcagcagg
     7741 ccccagagcg agacgtggtc cgggaccgtg ggccagccga ccgcgcccag gtagatcgtg
     7801 tcgaaccccg ctagcgtgtc cagggcgtcc tccggcatca tgcggccgtg ctccagataa
     7861 tactcgcatc cccagggata ctccgtgaac tccaggtcga gtgatgggtc gctctccgcc
     7921 gctgaccgca tgacccgcac cccggcgtct gttacctccg gcccgatgcc gtcgcccccg
     7981 atcacggcta cctttaccat ctcacgcgcc ctttccctgg ctccaacacc cggtttcatc
     8041 gtctggaccg cactctaaca accagccagc gggctgtcga accgccgtag atagccgcgt
     8101 taacggaaat ttaacgctcg tttggagtgg ccggtatata atccgcggac gattggaaag
     8161 gagccggaga tgccgaagac gaccctgaac ggtgttacgc tcgccgagag cgggagcaca
     8221 gaggtggtgg aggggaacca ttacttcccg cccgactcgg tgaaccgaga tcacctcgaa
     8281 gagagcgcga cgcgaaccac ctgtccctgg aagggagagg cgagctacta cagcctgcgg
     8341 gtggatggcg agaccgtcga ggacgccgcc tggtactacc cggagccgaa ggaagcggcg
     8401 gagaacatca gggaccacgt ggccttctac gccgacaagg tgcggataga ggactaacgg
     8461 ccttcggagg tcgggcggcc cggtagagtg acgggcggcc tatccacgag cccgaaagcc
     8521 ggacagggtc cggcgcgggg cgaggaggac agagagatgg acgagaggaa cctggatcgc
     8581 gcgaacaagg cggcccggca gatggcggag atcagccgcg agtcgtaccg ggcggtggtg
     8641 gagcgcgcct tcgccgcgcg agagagcaac gagcggctca cccggagctt cttcgaggag
     8701 ggcctggaac tctttcagga ccacgccgag ctgaaccgcc gcacgctgaa ccggatggcc
     8761 gaacaggctc aagagcatcg tgacgcgctc ctgagcctct cccgcgactc gctcacctcc
     8821 tacgacgact tcgttggctc cctctcaggc ttttacgacg aggtgaccgg agagcgggaa
     8881 cgcgaagagt agacgacttc tgaggatttt gccgcaagtt gcggcccaga aggttgcagg
     8941 ccggacacat tcctggtacc actagcaggg gcaactcgca caagctagag agacgaagta
     9001 acagcggatg aaatatgacc agagcgcgca ggccgccgca gggcaacctc gcgccgacgg
     9061 ttcgttcaag gtaaacccgc tcgcggccgg tgtgctgcgg cgcaacggtc tcaagtcatg
     9121 gacgaagccc gctccctcgc tctatcctca ccagtggagt tgggatagcg cgttcatcgc
     9181 gctgggtctc gcgcacgtgg acaaccggcg cgcgacccag gagctagaga cgctcttcgc
     9241 gagccagtgg tccaacggca aggtcccgca catcgtcttc aacccggagg cgcctccccg
     9301 cagctacttc ccggacgccg aacgctggaa ctcctcggcg ctctcagagc acgccccttc
     9361 gtccccgcac accagcgggt tgtgtcagcc gcccgtacac gccatcgcgg tgtggaggat
     9421 ctgggagact tcgcagggca aagaggggcg gcgcgtcgag gcctcactgc gcttcctgcg
     9481 ggagaattac ccgcggctcc tctcctggca ccgttacctg atgaccgccc gcgacccgga
     9541 agactccggg ctcgtcacga tctaccaccc gtgggagagc ggcacggata actctccccg
     9601 ctgggacagc gccctgcgcg aagtccgcgt cggggatttg ccgccctacg tgcgggccga
     9661 cttgcagcac gtcagcgacc cgtcgcaccg gcccaccgac tccgagtacg accgcttcct
     9721 gtggctcctg gagctcctca agcgcgctcg ctacgaggag gcggacatct atcgctcgca
     9781 tcccttcctc gtaaaagacg tgctcttctc cgcgatcctg gtcgccgcca acgaggccct
     9841 gctccagata gcggaggtag tagatgcccc cggcgaagac ctctcgctca tacgggcctg
     9901 gatcgaacgc ggccgccacg gcatagaagc gagctgggac cccgtcctga acctctccct
     9961 ggaccacgac ctgcgccggg gagaaccggc ccgcgtccgc acggtcgccg gcttcgcgcc
    10021 cctgatctcc ggcggcctcg ccccggaccg gctacaggct caactgcgga cgctcgactc
    10081 cccgcacttc gccggcaacc cggacctcga acgtccgctg ccgccttcaa ccagccccac
    10141 ggagcccggc tttcacccgc gcagctactg gcgcgggccc acctggccgg tcgccaactg
    10201 gctgctctgg tggtctctcc tgcgcgccgg agagcgcgtc cgggcggcgg ccctgcgggc
    10261 cgagatcctg gcccaactcg cccacggcgg attcgccgaa tacttcgaac ctttcaccgg
    10321 cgaacctcta ggctccaggg aacagtcctg gaccgccgcc gtcgccctcg acctcatcgc
    10381 cgccggagat gacgcaggaa acggcaccgg tggctagacg cctccagccg ctacaataga
    10441 gcttcgcgcg ggttcaggga gaggggcaga tgagcgcacc ggcggggctt tacgagttgg
    10501 tcgagaggtt cgaggggaac atccgggcgt acaagtccgg tgactacaac gagacgcagg
    10561 cgcggggcga cttcatagac ccgctgatgg gtcttctcgg ctgggacgtt cacaaccggc
    10621 aggggaaggc tcccgcctac cgggacgtgg tcgaggagga ccgggtgaag gttggcggcg
    10681 gtacgaaggc gccggactac ggcttctacg cgggcggctc gcggcggttc ttcctcgaag
    10741 ccaagaagcc ctcggtggat atcgcgggcg acgtgaaccc cgccgtgcag ctccggcgct
    10801 acgcctggtc ggcgaggctg ccgctctcca tcctcacgga cttcgaggag ttcgcggtct
    10861 acgactgccg cttcgagccg atccaggacg accgtcccgc gaccgcgcgg ctacagctcc
    10921 tcacctacga ccagtacccg gagaagtggg acgacatcta cagtctcttc tccggcgagg
    10981 cggtcctcgg cgggacgctc gaaaaatacg ccgaggagaa ccgcaagcgg cgcggcacgg
    11041 agaccgtgga cgcggcgttc ctgcgggaga tcgagggctg gaggagcgac ctcgcccgcg
    11101 acctcgccgc ccacaacaac ctgaactccc gagggctcaa cttcgccgtc cagatgacga
    11161 tcgaccgcat catcttcctc cggatggccg aggatcgcgg gatcgaggac tacggacgcc
    11221 tgctcgccct ggtgaacggc ggcgacgtgt acgggcggct actggaggtc ttccgggacg
    11281 cggacgaccg ttacaactcc ggcctcttcc acttccgcgc cgaacgcgga cgcgacgagg
    11341 agcccgacac cctgacgccg cacctgaagg tgggcgacgc gacgctcaag ggcat
//
LOCUS       DASSMB010000219        11311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1696142, whole genome shotgun sequence.
ACCESSION   DASSMB010000219 DASSMB010000000
VERSION     DASSMB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1696142"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /gene="manB"
                     /locus_tag="VFJ72_12955"
     CDS             <1..546
                     /gene="manB"
                     /locus_tag="VFJ72_12955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564118.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET7480413.1"
                     /translation="LEKEPGATILYSAVCSKALPELVEREGGTAIRTKAGHSIIKPQM
                     RSNDAAFGGEHSGHFYFRDNYFADSGIIAMLTVAELVARQDGPVSALLEPIDPYVRSG
                     EINSEVGDQQAKLHKVEEYYTREGDPDVDHLDGLTVDFGDWWFNLRPSNTEPLLRLNV
                     EASDAKTMERRRDELLRLIRD"
     gene            complement(626..1216)
                     /locus_tag="VFJ72_12960"
     CDS             complement(626..1216)
                     /locus_tag="VFJ72_12960"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7480414.1"
                     /translation="MAGKRKAAMMGARVGVKVAPKAGRIAWKAGKAQAKLTRHALSSR
                     EPRSVRFMKYGIFALVGFAIGALVGRTSGGSSSSDSATGIFGGSGHQRPQDENRTGAE
                     RDYSDPASGPLIGERHRRRVGNIPEQQEEVENRIRTRIGEDPRTREMPHVNVEVNDGV
                     ADLRGVASSEEVKQAAAEIAANVEGVREVRNHLTVA"
     gene            complement(1296..2144)
                     /locus_tag="VFJ72_12965"
     CDS             complement(1296..2144)
                     /locus_tag="VFJ72_12965"
                     /inference="COORDINATES: protein motif:HMM:NF037718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HET7480415.1"
                     /translation="MSTPLVERPSSPNAAAESRAAFAAWIGESPAGRRFGQVLIRPRP
                     GGGYSLCHIEDEGVADLQPFENPRAAREIAKLTGNGEYRPLKSAPNLRRGWAIRVADA
                     EELVVAMNGLYPAAVVHWYLQREGRLEITSYRQNAARQSGIYKRVQRLTDEGVQNAAR
                     ACCDDAVCLKKTLWDVDEKTPLEMERGEGEIPCPEPCSVFVSFARRVRLFEREDKRDL
                     DAIGLSPSEKEDLAALVEAAATGRVEMAREAEFEKPLNERRMRYRRLTLVPKLRRSSG
                     DLQGEK"
     gene            complement(2125..2886)
                     /locus_tag="VFJ72_12970"
     CDS             complement(2125..2886)
                     /locus_tag="VFJ72_12970"
                     /inference="COORDINATES: protein motif:HMM:NF002671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HET7480416.1"
                     /translation="MKALVIVGHGSHLNEDSSLPVYEHAERIRQTGEYDEVVECFWKE
                     EPSMRHVLDTVESDEVYVVPAFISEGYFTQQVIPRELGLEGPITRRGEKVIRYAGPLG
                     TYEGMADVILERTEDLMRGKETSGRTALVLLGHGTDLNKNSSGVIYQNAERIRRREAY
                     DVVEVGFLDQEPEIGEVVSGVEAENVILIPVFIAEGWHTRETIPEDLGLSGEVTVRED
                     RTIFYGAPVGTHPSMAALISARARETEEEVVHTAS"
     gene            complement(3007..3921)
                     /locus_tag="VFJ72_12975"
     CDS             complement(3007..3921)
                     /locus_tag="VFJ72_12975"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HET7480417.1"
                     /translation="MQEPSAPPVSEERVSDSAMTALTLAYSAPMPGLGSRYADGPVAH
                     EVRLLTAMPAAQALAAAARQAVGQTILDAALGSARVAGYADVRAAAYLAPLARAALTG
                     SSSGRVLAALGHHEIQPFLRAFETSAGIGADAPSDGSLAVSLRVALDSGTDATLRDAM
                     RFAAARDALAREYARDYEVTGDLARPALLATLGRAESSRAALVQSYLEVLAEVPDLDV
                     ARRAGTREAEDVSRMAKGVLKAGGVRSRRGLQAVINLDGILREDPRLAPSATEPPVMA
                     AAFLVSLEYGASALSQRLGPVSGSDRGR"
     gene            complement(3928..4386)
                     /locus_tag="VFJ72_12980"
     CDS             complement(3928..4386)
                     /locus_tag="VFJ72_12980"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7480418.1"
                     /translation="MPADVGEKAPDFTLPTDKWDNTVSLEDFTKEGPVALFFYPGDWS
                     SVCTDQMGKLQAEMGLFEEKGARVLAVSADSPWSHRAFAEERGIEFPLLSDFHKKVIE
                     DYGVKHEVGFPKRAYFIIDREGVIRAKKVEDTPSDQPEVEAVLADLDKAL"
     gene            4550..5692
                     /locus_tag="VFJ72_12985"
     CDS             4550..5692
                     /locus_tag="VFJ72_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HET7480419.1"
                     /translation="MTSADNQSLIAPDAFETGGPEGTLGAEEELWLADPETFKLANGA
                     QKILAATASGEFSGELIDCEIEANTGVHADPAGVVSDLISRRRVLLERADSLGRLLGT
                     SGTHPIGDWREQEIIDKPHYQRLKRKLGWLIRRNNTFSLHVHYAVQGREKALYVFNRV
                     REYLPHMLALSVNSPFWQGEYTDMHSARVLMFSRTYHRAGLPEPLGSWSDYTDYVDYL
                     KRSGLIDTLGEIWWDVRLHPGLDTVEIRAFDAQTDVSRNEALIAFAAAVCDMLVSEYE
                     SGDLKPFISNREIEENKWSAQRNGLDGEFIDHTTHEPVPTRSAIETLIDNAVERSDRD
                     LSTLATLLTQPTESARQLAVWRETGSILEVARDVAQRTRSATEAKI"
     gene            complement(5704..6048)
                     /locus_tag="VFJ72_12990"
     CDS             complement(5704..6048)
                     /locus_tag="VFJ72_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480420.1"
                     /translation="MAYTTVLLQTITKIEGEGLDSPKALNLTYVVPQGCTAGVQYMRA
                     GSTVTGMTNITLLKNGKVMRYIPVAAGSTVHVPLAIVDDLRPGTGIEVVAAAEGSGTL
                     ILDIGILEVGTA"
     gene            complement(6065..7495)
                     /locus_tag="VFJ72_12995"
     CDS             complement(6065..7495)
                     /locus_tag="VFJ72_12995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480421.1"
                     /translation="MASTSVESKKRVEGVKDGSNWIEKWNPEDEHFWENGGKRVARRN
                     LVFSIFAEFLGFSVWQLFSIVGALLPFIGFGFDSNQVFWLVAIPGLVGATMRFPYTFA
                     VQRVGGRNWTIISVLLLLIPTLILGFSVMNPATPYWVFVVAAAAGGFGGGNFSSSMTN
                     INYFFPNRLKGSALGINAAGGNIGVAIVQLLVPMLTTLGFSIVAIGGSQTKTDPATDA
                     TTSVFMQNAAFFWVPLILIAAVCAYFFMNSLHISQTPAREQALVLRRKHTWLMSWLYI
                     GTFGSFIGYSASFPALITVQFPENIEYAGFAWLGPLVGSLARPFGGWLADKVGGARVT
                     FWTFVVMIAAVLGVLYFLSAKSFPGFLGMFLLLFTMTGIGNGSTYRMIPFIFRTERAR
                     EAEGGDEETQDRLVRQGLKEGATVLGFAGAIAAYGGFIIPRAYGSSIEATGGPQTALT
                     AFIVFYATCIAVTWWFYYRRNAEIPC"
     gene            7861..10083
                     /locus_tag="VFJ72_13000"
     CDS             7861..10083
                     /locus_tag="VFJ72_13000"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HET7480422.1"
                     /translation="MANTKAAKQPGRWVKSTCPYCGVGCGVEAGLENGRMVQIRGDKS
                     HPANFGKLCPKPTGLPEALSSPDRLIYPQRRTASGDLKRVSWDEALAEISWKLEGTIE
                     QHGPEAAAFYISGQLLTEDYYAVNKLAKGFLGVNNVDSNSRLCMSSAVAAYTGAFGTD
                     GPPSAYADIAFAECVLLWGSNTADCHPITFGRIKRRKKDPAVSVIVIDPRRTATAEIA
                     DLHLPIKPGTDLALANAMLCVLIEEGFVDRDFVERRTSGFEATAETAYEWTPRKAARV
                     CGVAAEDIVRAARMFGEAKAALSMWTMGVNQSTVGTLKNRAIINLHLATGNIGKPGAG
                     PFSLTGQPNAMGGREVGGLAHLLPGYRLIANEEHRREVEEAWGVEPGSISEKPGLPAT
                     EIFRALEDGRAKFLWIAATNPAASMPDLARTRRALENAEFVVVQDAYPTETTAYADLI
                     LPVAQWGEKTGTMTNTERRVSIVEKVVEPPGEARPDWEIFAATARKMGFEDDFAWQNS
                     AEVYEEYKELTRDTVVDVTGLSHARLRRGPVQWPVPETLPFLELPARPMMERTRDING
                     EHPGTPRLYANKKFNTPDSRARFEPTPHQELHEPPTESYPLTLSNGRIKNQWHTMTRT
                     GRSEKLMRDLDGPFVEVHPEAAGDAGIKDGESARISSARGSFVARVVVTEDIEASTVF
                     APFHWGDLWTDGGSLNNTTHDAADPISKQPELKGAAVRLEPASRYESADVATRNGANA
                     "
     gene            10080..>11311
                     /locus_tag="VFJ72_13005"
     CDS             10080..>11311
                     /locus_tag="VFJ72_13005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7480423.1"
                     /translation="MTDRLRIVVIGNGNAGAALVDSLLAKDPAGVRITLYGGEEVGTY
                     DRVRLSEYMAGMVNLEDLGMRSDDWYREQNVDFRRGVLVEELDTKAQKVRGTDGEWVE
                     YDRLVFATGSSSSMPPIEGRDKKGVFVFRTVRDVEEMLEAAPERAVVIGGGLLGLEAA
                     YGLTTHGSEVTVLHRSPHLMNQQLDPAAGRMLGRQIEKLGIDFKVNAHTEEIRGNGRV
                     HGVKLKSGEYLRADMVVICTGIVPNTKPAGRAGIRANAGILVNEHMETSARNVFAVGE
                     CTEYNGQLIGLVAPAMEQVRVVVDTILGERKQVYKGSGAATKLKVAGVDLVSAGDAYG
                     RDEACEAIVSSNPIEGVYRKAVVKDGKVTGTILLGDVSAGPQMTEAVKSAMPAEEVAG
                     LVIGNVDASSAPLELPDEA"
BASE COUNT         2098 a         3737 c         3631 g         1845 t
ORIGIN      
        1 ctggagaagg agcccggagc cacgatcctc tacagcgccg tctgctccaa ggcgctcccc
       61 gagctcgtcg agcgcgaggg cgggacggct atacgcacca aagccggcca ctcgatcatc
      121 aagccccaga tgcgctcgaa cgacgcggct ttcggcggtg agcactccgg ccacttctac
      181 ttccgggaca actacttcgc agactccggc atcatcgcga tgctgaccgt cgcggagctg
      241 gtagcccgcc aggacggtcc ggtctcggcg ctactggagc cgatagaccc ttacgtgagg
      301 tccggcgaga tcaactccga ggtcggagac cagcaggcga agctccacaa ggtcgaggaa
      361 tactacacgc gggaaggcga ccccgacgtg gaccacctcg atggccttac cgtggacttc
      421 ggcgactggt ggttcaacct ccgcccctcg aataccgagc ccctcctgcg cctgaacgtg
      481 gaagcgagcg acgcgaagac gatggagcgc cgccgtgacg agctactgcg gctgatccgc
      541 gactgaccag cggaccatag agcaaagaga gaggcccccg ctatctcgcg ggggcctctc
      601 tcttatccgt agtgagggga taaagttacg cgacggtcag gtggttgcgg acctcgcgga
      661 cgccctcgac gttcgcggcg atctcggcgg cggcctgctt tacctcctcg gaggaggcga
      721 cgcctctgag atctgccacg ccgtcattga cctcaacgtt cacgtggggc atctcgcggg
      781 tgcggggatc ctcgccgatg cgggtccgga tcctgttctc tacctcttcc tgctgctcgg
      841 ggatattgcc cacacgcctc cggtggcgct caccgatcaa cggtccggaa gccgggtccg
      901 agtagtcgcg ctccgcgccg gtgcggttct catcctgcgg cctctgatgc ccgctgcccc
      961 cgaagatgcc ggtcgcggag tcgctggatg acgagccacc gctggtacgc ccaacgagcg
     1021 cgccgatggc gaaaccaacc agcgcgaaga tcccgtactt catgaaccgg acgctcctgg
     1081 gctctctgga actaagggcg tgtctcgtca gcttcgcctg cgccttgccg gctttccagg
     1141 caatcctgcc ggccttcggc gccaccttga ctcctactct ggcccccatc atcgccgctt
     1201 tcctcttgcc cgccatcact aacctccgtt ctcgtggatc taatcttgcc gtgctactac
     1261 ccgcgcgcaa ccttcgagaa accgcgtttc accggtcact tttcgccctg gagatccccg
     1321 ctagagcggc gcagtttcgg gacgagagtg aggcggcggt agcgcatccg gcgctcgttg
     1381 agcggcttct cgaactccgc ctcgcgggcc atctccaccc tccccgtggc cgcggcctcg
     1441 actagcgcgg cgaggtcttc cttctcgctc ggggagagcc cgatggcgtc caggtcgcgc
     1501 ttgtcctcgc gctcgaagag cctcacgcgc cgggcaaagg agacgaatac gctgcacggc
     1561 tcgggacagg ggatctcacc ctccccccgc tccatctcta gcggcgtctt ctcatctacg
     1621 tcccacagcg tcttcttcaa acacaccgcg tcgtcacagc acgcccgcgc cgcgttctgg
     1681 actccttcgt ccgtgagccg ctgaacccgc ttgtagatac cgctctgccg cgccgcgttc
     1741 tgccggtagc tggtgatctc cagacggccc tcccgctgca gataccagtg gacgactgcc
     1801 gccggataca gaccattcat cgcgaccacc agctcttcgg cgtccgcgac ccggatcgcc
     1861 cagccgcgcc tcaggttggg cgcgctcttg aggggccggt actcgccgtt gccggttagc
     1921 ttcgctatct cgcgggccgc ccgcgggttt tcgaagggtt gcagatccgc cacgccttca
     1981 tcctcgatgt gacagagtga ataaccgccg cccggtctgg gccggatgag gacctgcccg
     2041 aaccggcgtc ccgcggggct ctcgcctatc caggcggcga atgcggcgcg ggactcggcg
     2101 gcggcgttcg gggacgaggg acgctcaact agcggtgtgg acaacctctt cctccgtctc
     2161 tctggcgcgc gcggagatca gagccgccat tgatggatgg gtgccgaccg gtgcgccgta
     2221 gaagatcgtg cggtcctccc ggacggttac ttctccgcta aggccgaggt cctcggggat
     2281 cgtctcgcgc gtgtgccagc cctcggcgat gaagaccggg atcaggataa cgttctccgc
     2341 ctccacaccg gacacgacct ctcctatctc cggttcctgg tccaaaaacc cgacttcgac
     2401 cacgtcgtat gcctcgcgcc gccggatgcg ttcggcgttc tggtagatga cgccgctgga
     2461 gttcttgttc aggtcggtgc cgtgtccgag cagcacgaga gccgtccgtc cggaggtctc
     2521 cttgccacgc atcaggtctt cggtccgctc caggatcacg tcggccatcc cctcataggt
     2581 accgagcggc ccggcgtagc ggataacctt ctctcccctg cgcgtgatcg gcccttcgag
     2641 accgagctcc cgcgggatga cctgctgggt gaagtacccc tcggagataa acgcgggcac
     2701 gacgtagacc tcgtcggact ctaccgtatc gaggacgtgg cgcatggagg gttcctcttt
     2761 ccagaagcac tccacgacct catcgtactc tccggtctga cggatgcgct cggcgtgctc
     2821 gtagaccggg aggctggagt cctcgttcag gtgcgaaccg tggcccacta tgacgagtgc
     2881 tttcaaacgc ggaacgcctc cttccgtata tcgggttggt tctgaaattc tatcccagca
     2941 gcaccgagcg ctcccgtaaa gatttcaccc tcgattccgc tccggggtcc tatcggtcct
     3001 atgggcctac cgtccccggt cggacccgga cacgggcccg aggcgttggc tgagagcgct
     3061 cgcgccgtac tccagagaga cgaggaaagc ggcggccatt acgggaggct cggtcgcgga
     3121 aggcgcgagg cgcgggtctt cccgcagtat tccgtccagg ttgatcaccg cctgaagccc
     3181 gcggcggcta cgcacgcccc cggccttgag tacgcccttc gccatccggg agacgtcctc
     3241 cgcctcccgc gtcccggctc gtcgcgcgac atcgaggtcc ggcacctccg cgaggacttc
     3301 gaggtacgac tgaacgaggg cggccctgga ggactccgcg cgtcccagag tcgccagcaa
     3361 ggcgggacga gcgaggtctc cggtaacctc gtagtcgcga gcatactccc gagccagcgc
     3421 gtcccgtgcg gccgcgaagc gcatggcgtc ccgcagggta gcgtccgtgc cggagtccag
     3481 cgcgaccctc agcgatacgg cgagcgaacc gtcggagggc gcgtcggcgc cgattccggc
     3541 gctcgtctcg aaggcccgca ggaacggttg gatctcatgg tgtcccagcg cggccaggac
     3601 gcggccggag gacgaacccg tgagcgcggc gcgggcgagg ggagcgagat acgcggcggc
     3661 ccggacgtcg gcgtagcccg caacccgcgc ggaaccgagg gccgcgtcga ggatcgtctg
     3721 gccgacagcc tggcgcgcgg ccgcggctag ggcctgggcc gccggcatcg cggtcagcag
     3781 ccggacctcg tgggcgactg gcccatcggc gtagcgggaa cccagtcccg gcatcggggc
     3841 gctgtaggca agcgtcaggg cggtcatcgc gctatccgac acccgctctt cagagaccgg
     3901 cggcgcgctc ggctcctgca tcgggctcta gagcgccttg tcgaggtcgg ccaggaccgc
     3961 ctctacctcc ggctggtcac tcggcgtatc ctcgaccttc ttcgcccgga tcacaccctc
     4021 ccggtcgatt atgaagtagg cgcgcttcgg gaagccgact tcgtgcttta ctccgtagtc
     4081 ttcgatgacc ttcttatgga agtccgagag cagtgggaac tctatgccgc gctcctcggc
     4141 gaacgcccgg tgcgaccacg gcgagtcggc gctcaccgcc agtaccctgg cgcccttctc
     4201 ttcgaacaaa cccatctcgg cctggagttt gcccatctgg tcggtacaca cgctcgacca
     4261 gtctcccgga tagaagaaca gggcgacagg accctctttc gtgaaatcct ccagcgagac
     4321 cgtattgtcc catttatccg tcggtagcgt gaaatctggg gctttctctc caacatcggc
     4381 cggcataagt acctcctgta cggatcgcga atacgccgtg caacactctc attctaaaca
     4441 cgtcccgcgt aaagcgccgc cactacaggt taagatacgg gtcggcagaa agaaccggag
     4501 acgcgccgtc cgcggacggc gtgggcgatc tagaacacgg aggacggact tgaccagcgc
     4561 cgacaaccag tcgctcatag cgccagacgc cttcgagacc ggcggcccgg aaggcaccct
     4621 gggagccgaa gaagagctct ggctcgccga cccggagacc ttcaagctcg ccaacggggc
     4681 gcagaagatc ctcgccgcga ccgccagtgg cgagttcagc ggagagttga tcgactgcga
     4741 gatagaagcc aacaccggcg ttcatgctga tccagcgggc gtcgtatctg acctgatctc
     4801 ccgccgccgc gtactgctgg agcgggcgga tagtctggga cggctgctcg gtaccagcgg
     4861 cactcatccc atcggggact ggcgcgagca ggagataatc gataagcccc actaccagcg
     4921 cctcaagcgg aagctggggt ggctgatccg gcgcaacaac accttctccc tgcacgtcca
     4981 ctacgccgtg cagggccgcg agaaggctct gtacgtcttc aaccgcgtgc gcgagtacct
     5041 gccgcacatg ctggcgctct cggtcaactc tccgttctgg cagggcgagt acacggacat
     5101 gcactccgcc cgcgtcctga tgttctcccg cacctaccac cgcgccggtc tccccgaacc
     5161 cctcggctcc tggagcgact acacggacta tgtggactac ctcaagcgct ccgggctaat
     5221 agacacgctg ggggagatct ggtgggacgt cagactgcat cccggcctcg acaccgtcga
     5281 gatccgggcc ttcgacgccc agaccgatgt ctcccgcaac gaggccctca tcgccttcgc
     5341 cgccgccgtc tgcgacatgc tggtcagcga gtacgaatcc ggcgacctca agccattcat
     5401 ctcgaaccgc gagatcgagg agaacaagtg gagcgcgcag cgtaacggcc tggacggaga
     5461 gttcatagac cacacgaccc acgagcccgt cccgacccgg agcgccatcg agaccttgat
     5521 agacaacgcc gtcgagcgct cggaccggga cctctcgact ctcgccactt tgctcacgca
     5581 gccgacggaa tccgcgcggc agttagcagt gtggagagag acgggctcca tactggaggt
     5641 ggcccgcgac gtggcgcagc gcacacgctc ggccacggaa gcgaagatct aggtaatccg
     5701 cggctacgcg gtgccgactt cgaggatgcc gatatccagg atcagagtcc cggagccttc
     5761 ggcggcggcg acgacctcga tcccggtccc cggccgcagg tcgtccacga tagccagcgg
     5821 cacgtgtact gtgctcccgg ccgcgacggg tatgtaacgc attaccttgc cgttcttgag
     5881 aagcgttatg ttcgtcatcc cggtcacggt gctgcccgcc cgcatgtact gcaccccggc
     5941 cgtgcatccc tgcggcacga cataggtcag gttcagggct ttcggagagt ccagcccctc
     6001 gccctcgatc ttggtgatcg tctgcaagag cacggtcgta taggccacgc ggcacgcttc
     6061 ctttctagca ggggatctcg gcgttgcggc ggtagtagaa ccaccacgta accgcgatgc
     6121 aggtcgcgta gaacacgatg aacgccgtta gcgcggtctg cgggccgccg gtcgcttcta
     6181 tggaagagcc gtaagccctg gggattatga agccgccgta ggcagctatc gccccggcga
     6241 acccgaggac ggtcgcgcct tccttgagcc cctggcggac gagccggtcc tgcgtctcct
     6301 cgtcgccgcc ctcggcctcg cgggcgcgct cggtacgaaa gataaagggg atcatgcggt
     6361 acgtggagcc gttgccgatg cccgtcatcg tgaacaggag caggaacatc ccgaggaatc
     6421 cggggaatga ctttgcgctc aggaagtaga ggacgccgag gaccgcggct atcatcacca
     6481 cgaaagtcca gaacgtaacc cgcgcgccgc ccaccttgtc cgccagccag ccgccgaatg
     6541 gccgggccag ggagcccacc agcggcccga gccaggcgaa gccggcgtac tcgatgttct
     6601 ccgggaactg gacggtgatc agggccggga agctcgccga gtaccctatg aaagagccga
     6661 acgtcccgat gtagagccag ctcatcagcc acgtgtgctt gcggcgcagc accagcgcct
     6721 gctcgcgggc cggggtctgg gagatgtgca ggctgttcat aaagaagtac gcgcagaccg
     6781 cggcaatgag gatcagcggc acccagaaga aggcggcgtt ctgcatgaag accgaggtcg
     6841 tggcgtcggt ggcggggtca gtcttggtct gcgagccgcc gatggcgacg atggagaacc
     6901 caagggtcgt gagcatcggc accagaagct gcacgatagc caccccgatg ttgccgcccg
     6961 ccgcgttgat cccgagcgcc gagcctttca agcggttggg gaagaagtag ttgatgttgg
     7021 tcatgctgga ggagaagttg ccgccgccga agcctccggc cgctgccgcg accacgaaca
     7081 cccagtacgg cgtcgccggg ttcattaccg agaagccgag gatgagcgtc gggataagca
     7141 gcaagagcac gctgatgatc gtccagttgc ggccccctac ccgctgcacc gcgaacgtgt
     7201 acgggaagcg catcgtcgcc cccacgagcc ctggtatcgc caccagccag aagacctggt
     7261 tggagtcgaa cccgaaccct ataaaaggca acagagcgcc cacgatgctg aacaactgcc
     7321 acaccgagaa ccccaggaac tctgcgaaga tcgagaacac caggttgcgc cgggcaaccc
     7381 tcttcccccc gttctcccag aagtgctcgt cctcggggtt ccatttctcg atccagttgg
     7441 acccatcctt cacgccctcc acacgcttct tgctctcaac agaggtacta gccatccgcc
     7501 gcaccaactc cttcacctcg tagaatctcc agtcgcaacc agcccgtttc ggacccgcaa
     7561 catcactaat gcaggcatag cgatgtcgga tatggagcca atgctatgga caggacggga
     7621 taaagtcttt gtccatttgg cggaaatata ggggcgcctc aggacgatat ttatgtgcga
     7681 cggtgtggtt atcaagctgt agcaatatcc ggctctaaca gaaccgcata tatgtacatc
     7741 gggccgatgc gcggctgtgc ctcggtgtct aacctttgcc gagaccgtcg cggagcgcgt
     7801 gcaagcatgt ccgggcgtgg gtttttctgg aatgggcggc cggcaatccg aaggagagat
     7861 ttggcgaata ccaaggctgc aaaacaaccc ggacgctggg tgaaatccac gtgcccgtac
     7921 tgcggcgtgg ggtgcggcgt cgaggccggg ctggagaacg ggaggatggt ccagatccgg
     7981 ggcgacaagt cccatccggc gaacttcggc aagctctgcc caaagccgac cggccttccg
     8041 gaagcgctct cctcccccga tcggctcatc tacccgcagc gcaggactgc cagtggcgat
     8101 ctgaagcggg tctcctggga cgaagccctc gcggagatct cctggaagct cgaaggcacc
     8161 atagagcagc acgggccgga ggccgccgcg ttctacatct ccgggcaact gctcaccgag
     8221 gactactacg cggtcaacaa gctggcgaag gggttcctcg gggtcaacaa cgtggactcg
     8281 aactcgcggc tgtgcatgtc gagcgcggtg gccgcttaca cgggcgcatt cgggaccgac
     8341 ggaccgcctt ccgcatatgc ggacatcgcg ttcgcggagt gtgtattgct ctggggctcg
     8401 aacacggcgg actgtcatcc gatcacgttc ggcaggatca aacgccgcaa gaaagacccg
     8461 gccgtctcgg taatcgtaat agatccgcgc cgcaccgcga ccgctgagat cgccgacctc
     8521 cacctgccga tcaaacccgg caccgacctc gccctcgcca acgcgatgct gtgcgtcctg
     8581 atcgaagagg gcttcgtgga ccgcgatttc gtcgagcgcc gcacgagcgg gttcgaggcg
     8641 acggccgaga cggcatacga atggacaccg aggaaggcgg cccgcgtctg cggcgttgcg
     8701 gctgaggaca tagtccgggc ggcccggatg ttcggagagg cgaaggcggc cctgagcatg
     8761 tggacgatgg gcgtcaacca gagcacggtc gggacgttga agaaccgcgc gatcatcaac
     8821 ctgcacctgg ctaccgggaa cattggcaaa ccgggggcag gaccattcag cctcaccgga
     8881 cagccgaacg cgatgggcgg ccgcgaagtc ggcgggctcg ctcacctcct ccccggctac
     8941 cgcctcatag cgaacgagga gcaccggcga gaggtcgagg aggcgtgggg cgtggagccg
     9001 ggcagcatct cggagaagcc gggtctgcca gcgacggaga tcttccgagc gctggaagac
     9061 ggcagagcga agttcctctg gatcgcggcg accaatcccg ccgcttcgat gccggaccta
     9121 gcgaggacga ggcgcgccct ggagaacgcg gagttcgtcg tcgtacagga cgcttatccc
     9181 acggagacga ccgcttacgc ggacctgatc ctgccggtcg cccagtgggg cgaaaagacc
     9241 ggaacgatga ccaacactga gagacgagtc agcatcgtcg agaaggtagt agagccgccc
     9301 ggggaggcgc ggccggactg ggagatcttc gccgccaccg caaggaagat gggcttcgaa
     9361 gacgacttcg cctggcagaa ctccgccgag gtctacgaag agtacaagga actgacccgc
     9421 gacaccgtcg tcgacgtaac ggggctctct cacgcgaggc tccggcgcgg tccggtgcag
     9481 tggccggtcc ccgagacgct gccgttcctg gaactgcccg cgcggcccat gatggagcgg
     9541 acgcgggaca tcaacggcga gcatcccggc acacccaggc tctacgccaa caaaaagttc
     9601 aacacccccg acagtcgggc ccgcttcgag ccgacgccgc accaggagtt gcacgagccg
     9661 ccaacagaga gctacccgct gacgctctcc aacgggcgca tcaagaacca gtggcacacg
     9721 atgacccgga ccgggcgctc ggagaagctg atgcgggacc tggacggccc gttcgtcgag
     9781 gtccacccgg aggccgccgg agatgcgggc ataaaagatg gagagtcggc cagaatttct
     9841 tcggcgcgag gctccttcgt ggcgcgggtg gtcgtcaccg aagacataga ggccagcacg
     9901 gtcttcgcgc cgttccactg gggagacctc tggacggacg gcggcagcct gaacaacacc
     9961 acgcacgacg cggcggaccc catctccaaa cagcccgagc tcaagggagc agcggtgcgt
    10021 cttgagcccg cgagccgcta cgagagcgca gatgtagcaa ccaggaatgg agctaacgca
    10081 tgacagacag actcaggata gtggtaatcg gcaacggcaa cgccggggcg gcgctggtgg
    10141 atagtctgct ggcgaaggac ccggccggag tccggatcac gctctacggc ggcgaggagg
    10201 tcgggacgta cgacagggtc cgcctctcgg agtacatggc cgggatggtc aacctcgaag
    10261 acctcggtat gcgctccgac gactggtaca gagaacagaa cgttgacttc cggcgcggcg
    10321 tactcgtcga ggaactggac acgaaggccc agaaggtccg cggcaccgac ggcgagtggg
    10381 tggagtacga ccgcctcgtc ttcgcgaccg gttcttcctc ttccatgccg cccatcgagg
    10441 ggcgcgacaa gaagggcgtc ttcgtcttcc gcacggttcg ggacgtggag gagatgctcg
    10501 aagccgcgcc ggagagggcg gtcgtaatcg ggggtgggtt gctggggctt gaggcggcgt
    10561 acgggctcac gacgcacggc tcggaggtca cggtgctgca ccggtctccg cacctgatga
    10621 accagcagct agacccggct gcgggccgga tgcttgggcg tcagatcgag aagctgggca
    10681 tagacttcaa ggtcaacgct cacaccgagg agatccgggg caacggccgc gtccacggcg
    10741 tgaagctgaa gagcggcgag tacctgcggg ccgacatggt ggtcatctgc accggcatcg
    10801 tcccgaacac gaaacccgcc ggacgcgccg gcatccgggc gaacgccggc atcctggtca
    10861 acgagcatat ggagacgagc gccaggaacg tcttcgccgt cggcgaatgc accgagtaca
    10921 acgggcaact gatcgggctc gtcgccccgg cgatggagca ggtccgagtc gtggtggaca
    10981 ccatcctcgg cgagcgcaag caagtctaca agggctccgg cgccgcgacg aagctcaagg
    11041 tcgcgggcgt ggacctggtc tcggcgggcg acgcctacgg ccgggacgag gcttgcgagg
    11101 cgatagtcag ctcgaacccg atagaaggcg tgtaccggaa ggctgtcgtg aaggacggga
    11161 aggttaccgg cacgatcctg ctcggcgacg tctcagccgg cccgcagatg accgaggccg
    11221 tcaagagcgc gatgcccgcc gaggaggtcg cagggctcgt catcggcaac gtggatgctt
    11281 cgtccgcgcc gctggagctg ccggatgagg c
//
LOCUS       DASSMB010000220        27797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1454545, whole genome shotgun sequence.
ACCESSION   DASSMB010000220 DASSMB010000000
VERSION     DASSMB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 27797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27797
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454545"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VFJ72_13010"
     CDS             complement(<1..187)
                     /locus_tag="VFJ72_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480424.1"
                     /translation="MIEERQRVVEPDLSKAELRGVASRPASGSLKNFFRDMKSLLSFQ
                     ALKATVQEFMRDDALGLA"
     gene            517..1077
                     /locus_tag="VFJ72_13015"
     CDS             517..1077
                     /locus_tag="VFJ72_13015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7480425.1"
                     /translation="MNGVADEVLVRRVAGTGDEGALRELYDRYSGLIFGAGVRYLGDR
                     GLAEDLTQDVFTAVWRNADSFDPSRASFATWIYRITRNRATDLIRRRRARVRTVGDEF
                     VPEMGEDDQSGELSRSFDVAAALGKLSPVHREILTLAYFEGLSQREISRATGTPLGTV
                     KSRTTAALKALRESMNASAERWPRDE"
     gene            1070..1738
                     /locus_tag="VFJ72_13020"
     CDS             1070..1738
                     /locus_tag="VFJ72_13020"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7480426.1"
                     /translation="MNDLEHRRMRDLLGPYVLGGLDPEEELEITDHLSNCARCRDEEQ
                     ELRQAHEYMSDLAGVFEAPPPELKSRATRAVRLRGPRWAWLTAAAALVVLIGLAAAYS
                     TGVFDSQEAAAATLKPTQLAPEAGGEIWIKGSGQNVKLRLDAWGLPPCKGDQYYELWF
                     VEGKERLSAGSFTVGPSGKVKVDFTAPALSREYRNVGITSETTPDGPGPSKKKMLGGE
                     LNES"
     gene            complement(1791..5489)
                     /locus_tag="VFJ72_13025"
     CDS             complement(1791..5489)
                     /locus_tag="VFJ72_13025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7480427.1"
                     /translation="MMFLDFLRSRPRRVAVAAVIGLLFTLVFTGVVDLISIGGVHGGA
                     ADIVLLSVVLALVAAGAVLSGVVIHTLGELRRMHIGEREQMEELAKSEERFRLLAENA
                     RDVIYRYRISPERGFEYISPAAAAITGYTPEEHYADPELVFKMIHPEDRHLIEDSSRS
                     LESPLESPLVLRWRRKDGQTICTEQRNKPVRDQEGNLIAVEGIARDITQRKQAEERLR
                     EREEQLRLALEASSLGLWRQDLDTGEFIASPQASIMHGLPPGTPWSPDLALRVVHPED
                     RDSVRERVDRAIADGGSYDSEFRVVWPDESVRWIASRGRVYEGSDGRPRSLIGVVQDV
                     TARKQAEEALREAEEKYRGIFENSTSGIFHTTVDGRYRDANPALARLLGYDSPEELVA
                     TLTDLAHQLYENPEDRDKFTNALREHGSVKGYEVRVRRKNDTLVWLSVNARLVRDESG
                     NTVGYEGVVEEITERKESEEALRESEEKFRSAFDSASIGMALVGLDGRWLKVNPSLCE
                     ILGYPEDALLEMAFQDITHPDDLDCNLEQQDRLISGEVRSIRLEKRYIHRLGHVVWAL
                     LDASIVRDASGEQIYHIVQVQDITRSKKDREQLQRLSRQNELILKSAGEGIYGLDPRE
                     RMTFVNPTASRMLGYEPDELVGHHQHNIIHHASPDGTPYDTEDCPIHAALQDGNVHVS
                     SDQVFWRKDGTSFPVEYVSTPIREDGAVVGAVVTFSDITERRRTEEELARSEERFRLL
                     AEKMSDLVCLHSPDGRYVYLSPSCRRILGYDPEDLVGVDPYDLFHPDDARRIREVPHK
                     EALEGGTPDPIRYRIRRKSGEYIWLETLTEPILNEDGEVIRLQTSSRDVTESRRVEEA
                     LRESEERLRMTLQVSSVVLFQQDRDLRYTWVHNPQLGLSAEEMRGRTDLELLSSEDGA
                     LLTEIKTRALQTASGVREVVRMVSEGTAYYYDLTVEPLRNAVGEVVGLNGVAVDVTDR
                     QRAEEALAEAARAKTNLLADVSHELRTPLTVIRGNADIGLELERGCAHEEILEEILRE
                     SSTMSRMVEDLLFLARSDSAAPMFRLEPVSTRSFLSALESRATLLSQKRGAVLETNLN
                     GDGHLRIDPMRAEQAVLALVDNAARYSPAGEKVSLTSKSADGELRIEVADRGPGIPAN
                     DLPRVFDRFYRVEGSEGYRQQSGSGLGLSIAATIAEAHGGRIEAESRVGAGTRMSLYL
                     PLLDSSRPLDSTDNQPVALQDQVFSKDI"
     gene            5639..6316
                     /locus_tag="VFJ72_13030"
     CDS             5639..6316
                     /locus_tag="VFJ72_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480428.1"
                     /translation="MRILIIEDEKAIVRFLTRGLNAHGHQVLSAENGEDGVRLAVEDG
                     VELVLLDITLPNLDGHAALERIRRRRPDLPVLMLTARDDLRNKVSALDGGADDYLTKP
                     FVFEELLARIRALTRRSDQTRSAQIEAGGLRIDLLERRVWRGEDQLELSSREFALLEY
                     FMRHPGQVLSRQQILSAIWDYDFDPGSNIVDVYVRYVRNKIDTPGEPSYISTVRGAGY
                     RFEPPQK"
     gene            6636..8747
                     /locus_tag="VFJ72_13035"
     CDS             6636..8747
                     /locus_tag="VFJ72_13035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HET7480429.1"
                     /translation="MRLIVAEKPSVARDIAGVLGRSRKGKGSLAGDGWTVTWALGHLA
                     ELAPPDAYGDEYKKWRLESLPILPEKFKVRVNYKTREQFATVKGLMRDPSVTEIVNAC
                     DAGREGELIFAYLYGLSGCKKPVLRLWISSLTPEAIREGLDSLRDGHSMKPLEDAARS
                     RGEADWIVGMNATRAYSVRFSRPGNVLSVGRVQTPTLRLLVDRETEIQDFKPEKFWTV
                     YARFTREEATYDGVWFKDKQNRLKEKEAADQIADKVRGGTGTVKKAEKKTATEKPPLL
                     YDLTELQRNANARFGFTAERTLRAAQALYEEHKLITYPRTSSRYLSKDMVSTLKKRVE
                     AAGALPELSPFAGELLALDGLPISKRIVDDSKVTDHHAIIPTNKKSSRELPPDEAKVY
                     DLVARRFLAVFFPDARFENTTVVTEVRAETFLSKGRVVLEAGWRALYPDGVGGRKEKE
                     PPVLPPVQVGQEWDVAKVAVKEGETKPPPRYSESALLGAMETAGKFVEDEELRQRMKD
                     SGLGTPATRAATIERLIKVGYVEREKKALVPTQKGRALIGLLGESSLSSPELTARWEE
                     RLAKMEKGAARRDEFMADIGRFTSALVEEVKSLEGEKLAAPDRVSREPVGACPKCGSP
                     VVETKKAYGCSAWKKSGCDFAIWKTVSGKRLSAAQAKQLLTQRRTPQLKGFKSKAGKP
                     YAAALKLDEENKVKLEFAERG"
     gene            8870..9916
                     /locus_tag="VFJ72_13040"
     CDS             8870..9916
                     /locus_tag="VFJ72_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase family protein"
                     /protein_id="HET7480430.1"
                     /translation="MKAVLLTGYGGPEKLEYREDVPAPEPAAGEVLVKVGACGVNNTD
                     LWTREGAYGQADDPEAVGGWRRGEPMRFPLIQGADIVGRIVAVGDGVAGSRRGERVLI
                     DPTLYAGEGDGIVGVGAIGSERDGGFAEYVTVPASNAHAVESPLSDEELATFPTAYVT
                     AERMLKRARVAGGETVLVTGASGGVGSALVQLAKVRGAWVIAVVGPGKEAKVREIGAD
                     AVIARGVLDLPAAVAEVSGERPIDVVADVVGGDSFPHLLNILRSEGRYVVAGAIAGPS
                     VRLDLRTVYLKHLELIGSTLGAREDFADLVRYVSGGKVKPLLAGVYPLSEIRRAQSDF
                     QGKGFFGKLVLVPV"
     gene            10060..11304
                     /locus_tag="VFJ72_13045"
     CDS             10060..11304
                     /locus_tag="VFJ72_13045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7480431.1"
                     /translation="MRVIIVGAGLAGLTCAKVLNERGAEVVVFEASDGVGGRVRTDAV
                     DGFLLDRGFQVYFTSYPVARRHLDHDALDFRVFDPGAVIQRGRERSVLSDPLRDPKAL
                     VPSLLSGAANFEDKARIVALAAKVLTGTAASAGEENGESDSTTLDYLRKSGFSERFVD
                     SFFRPFYGGVLLNRSLTTSARVFRFTLRMLATGSTVVPARGMAGIPNQLASHLPPDAI
                     HLNSPVESLLREDGRVTGVRTNGEEHEADAVVVATDAPAAGKLTGEAVPEDSVGEVCI
                     YYETSGMGSGKKILLNASEDAFVNNAVEISNVSEHYAPRGRRLLYAVTLTGFDLPDAQ
                     LYRRGIEDLSDWRPEATFDPIAIRRIPYGQFAQPPGIHEKLPENRTATPGLVLAGEYT
                     EDSSINGSMLSGERAAKEVLGV"
     gene            11301..12050
                     /locus_tag="VFJ72_13050"
     CDS             11301..12050
                     /locus_tag="VFJ72_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7480432.1"
                     /translation="MTDITPALRIEKLTKTYSTGLRALDGVSLEIESGKFFGLLGPNG
                     AGKTTLINSVVSLARPDSGSVEVYGKDAFREFREARRMIGVSPQEINLDKFLTVEEAL
                     LFHAGYYGVPKAKAKERANELLERFGLTGKRDQRTNTLSGGMKRRVLFARALMHDPKI
                     LFLDEPTAGVDVELRYKLWEYIRELNRGGLTILLTTHYLEEAEALCEEIALINGGRIA
                     AQGTSGDLKEHYGARNIEEVYMKVIGHDTDG"
     gene            12034..12801
                     /locus_tag="VFJ72_13055"
     CDS             12034..12801
                     /locus_tag="VFJ72_13055"
                     /inference="COORDINATES: protein motif:HMM:NF011648.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480433.1"
                     /translation="MILTDRTFRTLLNREILRFTRVWMQTIVPQLLTSLLYLVVFGVA
                     LGGRIKEIHGIPYLEYILPGIALMSLITNSQMNSSWSVFDAKRERYIDEVLISPMSDL
                     QITLAYSLGGMLRGVVMGAGVFLVGIPFVGLHIEHPLLLVLIGLLASFIFSSFGTMAG
                     ALATRIEHISFLTSVVIQPLAFLGGVFYSVQMLPQSLQFVTLLNPIFHTVDATRYATL
                     GISDINPYPTIAIVFLLAALSFGGAWWAIHRGPNLRY"
     gene            12931..13365
                     /locus_tag="VFJ72_13060"
     CDS             12931..13365
                     /locus_tag="VFJ72_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480434.1"
                     /translation="MRRMVNDGIMGRMKEEREKIDDSAVADSGGGTLVLVAVQPRAYS
                     HAIAAVIEDMRPRLRVRVAEPDDLEDQVRRLDPDLVLCSQPNTFTPNGRPFWVEFYPY
                     AESPDDEIRVNGKGSRQRAIELADLLALLDGALAPDAALSEG"
     gene            13667..14893
                     /locus_tag="VFJ72_13065"
     CDS             13667..14893
                     /locus_tag="VFJ72_13065"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HET7480435.1"
                     /translation="MSRTGPVGRTSATRGTRARASSAAGDWPFVLAVFASSRAFFLLA
                     GALAAYFLPWAEPAGSPLEPPGLLNYWAHWDGAWYSEIATDGYDARYPASTAFFPAYP
                     MLLRLGSELGGGPALWGVLISLGATLLSLYFLYSIAEKLYDNHTARAATLAFAFFPTA
                     FFLNAVYTESLFLALTTGTIWAAYVRRDLLLAGILGALAAATRNLGVLLLIPLFLEWR
                     RNRREFGTSGLVALGLVPVGLLAYTVFLAGRFGDPFVFVRQQGEYWGRTLTSPLVTAQ
                     HAWEAAGEGARYILHPATLFLGDPSAPSLQASNTVSLVFLIGFVALMVIGLSVLPPGL
                     SIYALIITLLPVLTPTPSFPLMSMPRFVLGAFPIFLVIGYLLARRPTALMVWLVTSAL
                     VGVALTALFTTWRWVA"
     gene            complement(14906..17491)
                     /locus_tag="VFJ72_13070"
     CDS             complement(14906..17491)
                     /locus_tag="VFJ72_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HET7480436.1"
                     /translation="MTESRTYSHLVVVGSSAGGVEALSVLVSTLPPDFSAPVVIAQHL
                     DPHRESHLGEILARKSTLPVRTVTDHEPLEAGVVFVVPANRHVNVTDSEIDLRVDPSG
                     RPKPSIDLLLGSAAEVIGERLIAIILTGTGSDGAAGAAAVKKAGGTVIIQNPRTAQYP
                     GMPLSLAPNTVDIISELDRIGPILQDLLASVRAPSEPGEQEALRTLLEEVRERNGIDF
                     SSYKTPTIMRRLQRRIVATGSGNIEGYNEYLEENPEEYQQLVSSFLINVTEFFRDAEL
                     FDHLKTEMLPRLIEESRNRGNQLRLWSAGCATGEEAYSLAILMAEALGDELGRFKVRI
                     FATDIDEEAIAFARRGIYSHSALAGVSEDLINRYFVKDDGDYQVNKLIRGMVIFGQHD
                     LAQRAPFPNTDMVICRNVLIYFTTALQKRALQLFAYSLRDDGRLVLGKAESTSPLSEF
                     FVPEDRQRKIYRRRGDRFLMPPALLESPVPISPKKSPSPGENTARIPGPVQQAGRDQQ
                     QADRLLNNLLLKLPVGMVAVDRRYDIQFINNVARRLLSIHSPATGEDLIHLVQGAAYT
                     TLRSAIDAAFREDDPVEVDELAVEEPDTGEFRYLRMVIRAQSTSDAREPSKTVMIFIQ
                     DITNLARTRSDLAQRLQSVTAELEQEKSLNERLAQTNRQLDEGNQELTRVNEDLQTAN
                     EELLLSTEEAQAATEEVETLNEELQSTNEELETLNEELQATIEELNTTTDDLQARSME
                     LQSLARDSEREQARLEAILDSMDTAVLVVDAAGQDAFANAAYQRMFGERGLVCLDESG
                     EPLPPDEVPRHRATREELFNMEFLAETRDGARRRFEATGGPLRDDEDGRQGGVIHIRD
                     VKERG"
     gene            complement(17581..18321)
                     /locus_tag="VFJ72_13075"
     CDS             complement(17581..18321)
                     /locus_tag="VFJ72_13075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7480437.1"
                     /translation="MLQNEQPEEGPIEDKARLLSLISILQPLSWDEISDLARRMPEVR
                     LGKGQTLYPPKYRSGMLFLLLEGQVRLFEVTEGREFTLLVVRTGGLFGEMRLMDRRVQ
                     GAYAQALEPSRISLMSRDIFDKTVLRRPEVGLRMVDLLSERVAQYGGRLADIGIKEVH
                     ARLASLILRLVESEGALVGGEIRISTRYTHYQLGTMIGANREAVTNAMTQLRVSGVLT
                     TRHRYIHVRDVTALKKAAGTMSDVESEY"
     gene            18722..19201
                     /locus_tag="VFJ72_13080"
     CDS             18722..19201
                     /locus_tag="VFJ72_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480438.1"
                     /translation="MVHNGGGNGASGVLAEAVQNSYEAFVDGVAAAQARNMNFALGWI
                     GSSTEMLKAQAEINLRVMEAVAEQSRRQREVSRTLVEESVRTFVDFAYGPLPGTAEPE
                     AGGDENLPIDDFDRLTVEEIGSRLRGLDAREVEEIKTYEKRHKNRAMLMERLERALT"
     gene            19291..19491
                     /locus_tag="VFJ72_13085"
     CDS             19291..19491
                     /locus_tag="VFJ72_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480439.1"
                     /translation="MLSEEVRIGMRVKVSSAHIRSDLRGLTGVVEQMYGHPAYRALEV
                     RLENGRCELFWHYELKRENAFV"
     gene            19550..19903
                     /locus_tag="VFJ72_13090"
     CDS             19550..19903
                     /locus_tag="VFJ72_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480440.1"
                     /translation="MTRPERAPYWLIADCDGGHFVPFTVMSDGGGEMLPVFGHRDEAG
                     RFLRLAAAEGAWEVRETGAGELVSILSSLCGHVKRVVLDPSPEEGIEVWTELASLGRR
                     SFIESLLEREESAAS"
     gene            19914..20318
                     /locus_tag="VFJ72_13095"
     CDS             19914..20318
                     /locus_tag="VFJ72_13095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480441.1"
                     /translation="MIETKNILLVDDHGSFRNALARVLDWHPDFRVVNQAGTLAEARR
                     LAAQDGFDIAIIDLFLPDGSGMDLIRELRQAKRRAGLLLLTINLNYAQHAQAIEDGAD
                     GVLEKSVSVAEIIGAVERLVDKKSSERTLREA"
     gene            complement(20328..20672)
                     /locus_tag="VFJ72_13100"
     CDS             complement(20328..20672)
                     /locus_tag="VFJ72_13100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480442.1"
                     /translation="MTTAPRASAISGVQIGVIVLTAATALIHLYLGLQGFPIFILNGL
                     GYLGLLGALYLPLPQLARYHNAARWALIGYAALTIFLWVLIGLRTPIGYTDKLIEVAL
                     IALLVLEGRRQR"
     gene            complement(20699..21904)
                     /locus_tag="VFJ72_13105"
     CDS             complement(20699..21904)
                     /locus_tag="VFJ72_13105"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480443.1"
                     /translation="MPLAGDRRTPLVFLFVTVFVDMIGYGIVVPLLPFFARQYAEGAL
                     LVGLLGSLYAMMQFAGGPFLGGLSDRIGRRPVLLMCLLGASLAYLLLGLAGSLSALVL
                     AVVLAGGASGTLATAQAYIADSTTKDDRARGLGLIGAAFGLGLMAGPALGGLLSFHSL
                     HAPAFAASALAFSNAVFGYLALPESHPPRLRRSVPLLRLNPVSQLSGVLKMPGIRALL
                     AAILLLNLSFAGLVSNFPLFSSARFEWGAAENAFFFAFVGVCAVVTQGALIGRLQPWL
                     GEERLLIGGLALMALNLALMALIPAGFLLYPVVGLLALGAGLAIPSVTSIVSNRASDY
                     EQGRLMGGVQAILSLALILGPALAGLVFDYVGVPAPYLMGGALAALALTVAAKSLAAP
                     SEKAVQQTV"
     gene            complement(21904..22803)
                     /locus_tag="VFJ72_13110"
     CDS             complement(21904..22803)
                     /locus_tag="VFJ72_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7480444.1"
                     /translation="MDNVFGLPAGPFRLPVPERQEHLDTLTRVCVDDLFSAFGLREKR
                     RSRAALEAVARIPARRLARQVLTYDAIVGDSGLGAGGAWALKQLARRAEVEGAESVPL
                     EGPLLVVSNHPGLADAVALFAATPRPDLRVIAAERPFLGALPNTSRHLITVAETSPGR
                     FGPVRAATRHLRDGGAVLTFPGGRIEPDPAILPGAIESLGSWSASVDLFAKLTPGLAI
                     VPVAVSGVISPAALRNPLTRLRRRRSDQEWLAATLQMLTPALRGVTVRVAFGNPIVAG
                     KKEAVGGAVRMEMRRLIESGGAR"
     gene            complement(23033..23419)
                     /gene="folB"
                     /locus_tag="VFJ72_13115"
     CDS             complement(23033..23419)
                     /gene="folB"
                     /locus_tag="VFJ72_13115"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET7480445.1"
                     /translation="MSEDRILLEGMVFTGRHGTLPAERELGQPFVVDIEMRLDLRPAG
                     SSDDLTKTVDYSEVHRMSRAIVEGEPVGLTETLAERIAAAVMENFPLVEAVKVKVTKP
                     HVRLDGTVLSGSAVEVLRRRETAASE"
     gene            23549..24250
                     /locus_tag="VFJ72_13120"
     CDS             23549..24250
                     /locus_tag="VFJ72_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HET7480446.1"
                     /translation="MTVSYAQTLDGRLATAGGSSQWISCRDSLRFAHALRAAHDAIIV
                     GAGTACKDDPRLTVRLVPGDDPLRVVVDSTLRTPLAAAVLADGAAKGTVMAVTGRAPE
                     DRCEAARALGATVLRLPEDGAGRVDLRALLSELREMGVESAMVEGGAALITGFFRERL
                     VRRLAVCIAPKILGSGIEAVGDLGVRDLADSLTLTGTTIQSCGVDLILDSRVEYPNGS
                     PNGSPNGNGAGARGA"
     gene            24234..25259
                     /locus_tag="VFJ72_13125"
     CDS             24234..25259
                     /locus_tag="VFJ72_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HET7480447.1"
                     /translation="MPEGLEARALWFAAPRTAELRSENAPAPGPGEVRVRALASAISG
                     GTEMLVYRGEVPEGMQLDLPTLAGSYGFPIKYGYAAVGHVLDIGSHVQRLSPGDPVFV
                     HHPHQDFFTVPAHTPVRLPEGLDPLLGVYCANLETALNIVHDTPLRLGETALVLGQGV
                     VGLLVTQLLKISGAGEVLVVDPFRKRRDLALAVGADAAFKPDEELGTRLREMTGGRGA
                     DVVVEVSGSGEALQAGIEHVADEGTVVVASWYGAKPVSLSLGGHFHRGRVKIRSSQVG
                     RISPELGPRWDRDRRMVAVLKLLPRLRLEGLISHRIPFEKAPEAYRLLDENPEEVVQV
                     ILTYERS"
     gene            25407..25784
                     /locus_tag="VFJ72_13130"
     CDS             25407..25784
                     /locus_tag="VFJ72_13130"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET7480448.1"
                     /translation="MYEIYVAARFEAAHRLIGDFGPAARTHGHTYRMEVIVRGERLKE
                     DETLYDIGELRPAVDGLAASLHYRDLNEVPGLEDVNTTAEAVARYCWEKLAPPLRGNG
                     LDSLAVRIWESSDVYAAREDSLG"
     gene            25788..26861
                     /locus_tag="VFJ72_13135"
     CDS             25788..26861
                     /locus_tag="VFJ72_13135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7480449.1"
                     /translation="MRVAFVTVGDTSRMTGGYLYNARVMDGLRERGVRVEEIVASSAS
                     PEEQRTAARRGDALDPTDFDVVVVDALARIAVAPHLDRWRMECPVVAMVHELPSIAGG
                     ESGAEAAAREREYEEPLLRSDLFIAVSDHGRRLLEIQGVPHPRVYIVPPGFDRLPAPA
                     EPVDNLDGTVRALCVAQWIPRKGILTLVEAWTRRERPGAALELIGETDADPEYTAQVR
                     ETIAAAPDSSLVVSGTVDDAELMRSYAAADLFALPSRYEGYGVVYAEALSFGLPVVAC
                     EVGPVPELVGRDAAVLVPPDDPVALAEALDLLLEDPDLRRRMSGAARRRAERLPRWED
                     TVSGFETALRAAVRDGARRTGAR"
     gene            26871..27767
                     /locus_tag="VFJ72_13140"
     CDS             26871..27767
                     /locus_tag="VFJ72_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7480450.1"
                     /translation="MDRRDLREQNRLSWNAVVGAHDSHRGDLVGFFRGGGNTLFPEER
                     ELLGDLEGKTLAHLQCNTGGDSISLAKLGAVVTGVDISDEAVLSARELSEKTGIPAAF
                     ERADLYDWLEESARGGHRFDVVYASYGVVCWLPDLAGWAAGIAAILEPGGRFVLVDFH
                     PVADMFDEDWNHARGYPHGGEPLPLGEGVGDYVAESGGGLTPAGFAEGVRSFQNPEPA
                     HLFRWGLGEVVTALAGAGLRITALKEYPYSNGERHFARMRELKGRRMVPPEDVPAVPL
                     MYGIRADKLGYGEKKNASAAMT"
BASE COUNT         4738 a         8842 c         9312 g         4905 t
ORIGIN      
        1 ccgcgagccc cagggcgtcg tcccgcatga actcctgcac ggtcgccttg agagcttgaa
       61 aactgagcag gctcttcatg tcgcggaaga agtttttcaa cgacccgctc gccggacggc
      121 tggcaacccc ccgaagctcg gctttagaaa gatccggttc caccaccctc tgcctctcct
      181 ctatcaacca ttcctatttt accggaaatg caactttgtc ctcagaagtc ttcgccgctg
      241 gtgggcaccc tatctacacg ccgtatcaac gggatttctc ctcgtagttc cctcgcacgt
      301 acctggttgc gcgggctgtt tcgcggttcc gggtagacgg tggcgctcgg ccgggtattc
      361 ttgcgtgttt cgggtcatcc gaaagcgttt ccgtagcgta taaaagttca tatggatgct
      421 ggtgtttaca ggagtttcgg cggctggtcg cgcatctttg ggcgttcggg ctgggaaccg
      481 gctatcattc tctatggtgg ctttgagagg ttcggaatga acggcgttgc ggacgaggtc
      541 cttgtacggc gggtcgccgg gacgggcgat gagggggctc tgagagagct ctacgaccgg
      601 tactcgggtt tgatctttgg cgcgggcgtc cggtatctgg gggaccgggg gctggcagag
      661 gacctgacgc aggacgtctt cacggctgtg tggcggaacg cggacagctt cgacccatcg
      721 cgggccagct tcgccacctg gatctaccgc atcacccgca accgggcgac cgacctgatc
      781 cgccgccgcc gggcgcgggt gcggactgtc ggagacgagt tcgtgccgga gatgggcgag
      841 gacgaccagt ccggagagct atcccggagc ttcgacgtgg cggcggctct cggaaagctc
      901 tcgccggtcc accgcgagat actgaccctt gcgtatttcg agggactgtc gcagcgcgag
      961 atctcacgcg ccaccggcac gccactcggc acggtgaaga gccgcaccac ggcggccctc
     1021 aaggcgctgc gcgagagtat gaacgccagc gcggaacggt ggccccgcga tgaatgacct
     1081 ggaacaccgc cggatgcgag acctcctcgg gccctacgtg ctcggcggcc ttgacccgga
     1141 ggaagaactg gagatcacgg accaccttag caattgtgcg cgatgccggg acgaagagca
     1201 ggagctgcgg caggcccatg agtatatgtc cgacctcgcg ggcgtcttcg aggctccgcc
     1261 cccggaactc aagtcccgcg ccacgcgcgc cgtgcgtctg cgcggtccgc gatgggcatg
     1321 gctaaccgcg gcggcggcgc tcgtcgtcct cattggtctt gcggccgcgt attcaaccgg
     1381 ggtgttcgac tcccaggaag cagccgccgc taccctcaag cccacgcagc tcgcgcccga
     1441 agcgggtggt gagatctgga taaagggcag cggacagaat gtcaagcttc gcctcgacgc
     1501 ctggggtttg ccaccgtgca agggagatca atactacgag ctctggttcg tggagggtaa
     1561 ggagcgcctg agcgccggca gcttcaccgt cgggccgagc ggcaaggtca aggtagactt
     1621 taccgccccc gcgctctcca gggaataccg gaacgtcggg ataacctctg agaccacccc
     1681 tgacggtccc ggtcccagca agaagaagat gctcggcgga gaactgaatg agtcgtgagc
     1741 ggcgcgccac cagaagctgc ccgtaagcag taaccaagca gtaaccgcgc tcagatgtcc
     1801 ttcgagaaaa cctggtcctg tagagcgacc ggctggttat cggtggagtc cagcggtcgt
     1861 gaggaatcca gcaacggcag gtagagtgac atccgggtgc ccgctcccac gcggctctcg
     1921 gcctcgatgc gcccgccgtg ggcctcggct atcgtggcgg ctatggagag gccgaggccg
     1981 gagccgctct gttgccggta gccttccgag ccctctactc ggtagaacct gtcgaacacg
     2041 cgcggcaggt cattggccgg tattccgggt ccccggtcgg cgacctcgat ccgcaactcg
     2101 ccgtcggcac tcttggaggt gaggctcacc ttttctccgg cggggctgta ccgggcggcg
     2161 ttgtccacca gggcgaggac ggcctgttcg gcgcgcatgg ggtcgatgcg gagatggccg
     2221 tcgccgttca ggttggtttc gagtaccgcg ccccgtttct gggagaggag tgtcgcccgg
     2281 ctctccaggg cgctcaggaa agatcgcgtg gagacgggtt ctaaccggaa catcggggcg
     2341 gcggagtccg agcgggccag gaagagcagg tcttctacca tccgcgacat ggtcgaggat
     2401 tcgcgcagga tctcctccag tatctcctcg tgggcgcagc cgcgctcgag ctccaaaccg
     2461 atgtcggcgt tgccccggat cacggtgagc ggtgtgcgaa gctcgtggga gacgtcggcg
     2521 agcaggttcg tcttggcgcg cgcggcctcg gctagcgcct cctcggcgcg ctggcggtcg
     2581 gtgacgtcaa ccgcgacccc gtttaggcca acgacctcac ccaccgcgtt gcgcaggggc
     2641 tcgacggtca ggtcgtagta gtaggcggtg ccctccgata ccatgcggac tacctcgcgg
     2701 acgccggacg ccgtctggag ggctcttgtc ttgatctccg tgaggagcgc gccgtcttcg
     2761 gaggagagta gctccaggtc cgtgcgcccg cgcatctcct cggcactcag gccgagttgc
     2821 gggttgtgca cccaggtgta ccggaggtcc cggtcctgct ggaagaggac cacggacgag
     2881 acctggaggg tcatccgcag ccgctcctcg ctctctctga gggcctcctc cacccggcgg
     2941 ctctcggtca cgtcgcgcga ggaggtctgc aggcggatga cctcgccatc ctcgttcagg
     3001 atcggctcgg tcagggtctc cagccagatg tactcgccgg acttcctgcg gatgcggtac
     3061 cggatcgggt cgggcgtacc gccttcgagg gcttccttgt gggggacctc gcggatgcgc
     3121 cgcgcgtcat ccgggtgaaa gagatcgtaa gggtccacgc cgacgagatc ctcgggatcg
     3181 tagccgagta tgcgccggca ggacgggctg aggtagacat agcggccgtc gggactgtgc
     3241 aggcagacaa ggtcgctcat cttctccgcg agcagccgga agcgctcctc gctcctcgcc
     3301 agctcttctt ccgtgcgccg ccgctcggtg atgtccgaga aggtcacgac cgcgcctacc
     3361 accgctccgt cctcccggat cggggtgctc acatactcca cggggaagct tgtgccgtcc
     3421 ttgcgccaga agacctggtc gctggagacg tgtacgttgc cgtcctgcag ggcggcatgg
     3481 atcggacagt cttccgtgtc gtagggagta ccatccgggc ttgcatgatg gatgatgttg
     3541 tgctgatggt ggcctacaag ttcatcgggt tcgtaaccga gcatccggga cgctgtgggg
     3601 tttacgaacg tcatccgctc tcgcggatcg agcccgtaga tcccctctcc ggccgacttg
     3661 aggatcaact cgttctgccg gctgaggcgc tggagttgct cccggtcttt cttgctccgg
     3721 gtgatgtcct ggacctggac gatgtggtag atctgctcgc ctgatgcgtc gcgcactatc
     3781 gaggcgtcta gcagcgccca caccacatgc cccagcctgt gtatgtaacg tttctccagg
     3841 cggatagagc ggacctctcc cgagatgagc cggtcctgtt gttcgaggtt gcaatcgagg
     3901 tcgtcggggt gggtgatgtc ctggaaggcc atctccagca gcgcgtcttc cgggtagccg
     3961 aggatctcgc acagagacgg gtttaccttc aaccagcggc cgtccagccc gacgagagcc
     4021 atccctatgg acgctgaatc gaacgcgctc cggaacttct cctcgctctc ccgcagcgct
     4081 tcttcgctct ccttgcgctc ggtgatctcc tcgactacgc cctcgtagcc gacggtgtta
     4141 ccggactcat cgcgcacgag acgggcgttg accgagagcc agacgagcgt atcgttctta
     4201 cgcctgacgc gaacctcgta acccttgacc gagccgtgtt cgcgtaaggc attggtgaat
     4261 ttgtcgcggt cttcggggtt ttcgtagagc tgatgcgcta agtctgtcag ggtggcgacg
     4321 agttcctctg gcgaatcgta gcccaggagg cgggccagcg cggggttcgc gtcccggtaa
     4381 cgtccatcca cggtggtatg aaaaatgccg ctggtggagt tctcgaagat gccgcgatac
     4441 ttctcctctg cctcgcgcag ggcctcctcg gcctgcttgc gggcggtcac gtcctggacg
     4501 accccgatca gagacctcgg acgcccgtcg gaaccttcgt agacccgccc gcgggaagct
     4561 atccatcgca cggactcgtc gggccagacg acccggaact ccgagtcgta ggaaccgcca
     4621 tcggcgatgg cccggtctac cctctccctg actgagtcgc ggtcctcggg atgcacgacg
     4681 cggagggcga ggtcggggct ccacggtgtt ccgggcggga ggccgtgcat gatgctcgcc
     4741 tgcggggagg cgatgaactc gccggtatcc aggtcctggc gccagagccc gagcgatgaa
     4801 gcctccagcg ccagccggag ctgctcttcc ctctcccgca gcctctcttc cgcctgttta
     4861 cgctgggtta tgtcgcgggc tatgccctca acggcgatga ggttgccttc ctggtcgcgg
     4921 acaggtttgt tgcgctgctc ggtacagatg gtctggccgt ccttgcggcg ccagcgcagc
     4981 accagcggcg actccagcgg agactccagc gaccgcgaag aatcctctat gagatgccgg
     5041 tcctcggggt ggatcatctt gaagaccagc tccgggtcag cgtagtgctc ttctggggtg
     5101 tatccggtga tggccgccgc cgcggggctg atgtactcga acccgcgttc gggagagatg
     5161 cggtagcggt agatcacatc ccgcgcgttc tccgccaaca gccggaaacg ctcctcgctc
     5221 ttcgccagct cctccatctg ctcccgctcc ccgatgtgca tcctgcgcag ctctccgaga
     5281 gtgtgtatca ccacgccgga gaggacagct cccgccgcta ccagcgccaa caccacggac
     5341 aacaggacta tatccgcagc gccgccatgc acgccgccga tagagatcag gtccaccacg
     5401 cccgtaaaga cgagggtaaa cagcaacccg atgaccgccg cgaccgcgac cctccgcggc
     5461 ctcgacctca agaagtccag aaacatcatc cgcataccct ccagagagaa ccggtctctg
     5521 tgcttgctcc gcagtttgcc ttctggctcg ccctttgatc tccgggcgag ttgtcatata
     5581 ttatactttg ggcgtagcag cgcgccgccc agcgcgccac gggtgtatgg ggaatacatt
     5641 gcggatattg attatcgagg acgagaaggc catcgtgcgc tttctgacgc gcgggttgaa
     5701 cgcccacggc catcaggtcc tcagcgcgga gaacggcgag gacggtgtgc gcctggcggt
     5761 cgaggacggg gtggagctgg tgctgctgga catcacgctg cccaacctcg acggacacgc
     5821 ggccctcgaa cgcatccgcc ggcgccgccc ggacctgccg gtgctgatgc tcaccgcgcg
     5881 cgacgacctg cggaacaagg tcagcgccct ggacggcggc gcggatgact acctgaccaa
     5941 acctttcgtc ttcgaggagt tgctcgcccg catccgggcc cttacgcggc gctcggatca
     6001 gactcgctcc gcccagatag aggccggcgg gttgcggata gaccttctgg agcgccgggt
     6061 ctggcgcggg gaggaccagt tagagctttc gagccgcgag ttcgccctgc tcgaatactt
     6121 catgcgccac cccggacagg tcctctcccg gcagcagatc ctctccgcca tctgggacta
     6181 cgacttcgac cccggctcca atatcgtgga cgtctacgtc cgctacgtca ggaacaagat
     6241 agataccccc ggcgaacctt catatataag caccgtccgc ggcgccggat accgcttcga
     6301 gccgccacag aaataaccca aaggcgtaca ccgaaagttg tatacaaaga tgagaagatt
     6361 ctaatctccc tctcacagtg ctctcatatt ggtttgagat acttcttttc gagagggtcg
     6421 caggcgtggc acccgtatcg ctacccatcg tgttaccgcc acctgcgcct cacaaattaa
     6481 agagtccggg gtcactggtg gatcctcctt tccctttccc cacctatcac ccatcaaaga
     6541 ccacctcctc ggactcttct ctttcgaaca ctccgtggtg cggcgccgga acatgaggga
     6601 tgggctcccg gactccatcc cctatactcc tctccatgag acttatcgtc gccgagaaac
     6661 cgtccgtggc gcgggacata gccggcgtcc tgggaaggtc gcgcaagggc aagggctcgc
     6721 tcgcgggcga cggctggacc gtcacctggg ctctggggca cctggcggag ctcgcgccgc
     6781 ccgacgccta cggggatgaa tacaagaagt ggcggctgga gagcctcccg atcctgccgg
     6841 agaagttcaa ggtgcgggta aattacaaga cgcgcgagca gttcgcgact gtgaagggcc
     6901 tgatgcgcga cccgtccgtc acggaaatcg tcaacgcctg cgacgccggg cgggaaggtg
     6961 agctcatctt cgcctacctc tacggtctct ccggctgcaa aaagccggtc cttcggctct
     7021 ggatctcctc gctcaccccc gaagccatcc gcgaaggtct tgactctctg cgcgacggac
     7081 actccatgaa gccgctcgaa gacgcggccc gcagccgcgg tgaggccgac tggatcgtgg
     7141 gcatgaacgc cacccgcgcc tactccgtcc gcttctcgcg ccccggcaac gtcctctccg
     7201 tcggccgcgt ccagaccccg accctccgcc tcctcgtgga ccgcgagacc gagattcagg
     7261 acttcaaacc ggagaagttc tggaccgtct acgcccgctt cactagagaa gaagccacct
     7321 acgacggcgt ctggttcaag gacaagcaga accgcctgaa ggagaaggag gccgcggatc
     7381 agatagcgga caaggtacgc ggcggcaccg gcaccgtaaa gaaagccgag aagaagaccg
     7441 ccaccgagaa accgccgctc ctatacgacc tgacggaact ccagcgtaac gccaacgccc
     7501 gattcggctt caccgccgag cgtaccctgc gcgccgccca ggcgctctac gaggagcaca
     7561 agctcattac ctaccctcgc acctccagcc gctacctctc caaagacatg gtctccaccc
     7621 tcaagaagcg cgtcgaagcc gccggggcgc tgccggagct atcgccgttc gccggggagt
     7681 tgctcgcgct cgacggcttg ccgatcagca agcgcatagt ggacgactcg aaggtgacgg
     7741 accaccacgc catcatcccg accaacaaga agtcctcgcg ggagctgccg ccggacgagg
     7801 cgaaggtata cgacctggtc gcgcgccgct tcctggccgt gttcttcccg gacgctcgct
     7861 tcgagaacac caccgtcgtc accgaggtgc gcgcggagac tttcctctcg aagggccggg
     7921 tcgtgctgga ggccgggtgg cgggcgctct acccggatgg ggtcggcggc cggaaggaga
     7981 aagagccgcc ggtcttgcct ccggtacagg ttgggcagga gtgggacgtg gcgaaggtcg
     8041 cggtcaagga gggcgagacc aagccgccgc cgcgctactc ggagtcggcg ctgctcgggg
     8101 cgatggagac ggcggggaag ttcgtggagg acgaggagct tcgccagcgg atgaaggact
     8161 ccgggctcgg caccccggcc acgcgggcgg cgaccatcga gcggctcata aaggtcggct
     8221 acgtggagcg cgagaagaag gcgctcgtcc cgacacagaa gggccgcgcg ctcatcgggc
     8281 tgctcggcga aagttcactc tcctcacccg agttgacggc ccggtgggag gagcgcctcg
     8341 cgaagatgga gaaaggcgcc gcccgccgcg acgagttcat ggccgacatc ggccggttca
     8401 cgagcgcgct cgtcgaggag gtaaagagcc tggaaggcga gaagctggcg gcgccggatc
     8461 gggtcagccg ggagccggtc ggcgcgtgcc caaagtgcgg ctcgccggtg gtcgagacga
     8521 agaaggcata cgggtgttcg gcctggaaga agtccggatg cgacttcgcc atatggaaga
     8581 ccgtctccgg caagcgtctg agcgccgccc aggcgaagca actcctcacg cagcgccgta
     8641 cgccgcagct aaagggtttc aagagcaagg ctggaaagcc ctacgccgcc gcgctaaagc
     8701 tggatgagga gaacaaggta aagctggagt tcgcggagcg tggctaaaag caaacctcat
     8761 cgttgaatcc gttgtcgcag cgtggcgcgg tcctggacgg gggcgctatg atgcttccga
     8821 cgcgcgacac aagaacgaga gccaaaatga cgaggaggct cgggtcagcg tgaaggcggt
     8881 tctgctcacc ggttatgggg ggccggagaa gctcgagtat cgggaggacg tccctgcgcc
     8941 ggaacccgcc gccggggagg ttcttgtgaa ggtcggcgcc tgcggtgtga acaacaccga
     9001 cctctggacc agggagggag cctacgggca ggcggacgac ccggaggcgg tgggcggctg
     9061 gcgccgcggg gagccgatgc gctttcccct gatacagggc gcggacatcg tcgggcgcat
     9121 cgtcgcggtc ggagacggag ttgccgggtc gcggaggggt gagcgggtgc ttatagaccc
     9181 gaccctctac gccggggagg gggacgggat cgtgggcgtc ggagcgatcg gaagcgaacg
     9241 cgacggcggc ttcgcggagt acgtcacggt ccctgcgagt aacgcgcacg ccgtcgagag
     9301 tcctttgagc gacgaagagc ttgcgacttt ccccacggcc tacgtcaccg cggagcgcat
     9361 gctgaagcgg gcgagggtcg cgggagggga gaccgtcctc gtgacgggcg cctcgggagg
     9421 ggtgggctcc gccctggtgc agcttgccaa agtgaggggc gcgtgggtga tcgcggtggt
     9481 agggccggga aaggaggcca aagtccgcga aatcggcgcg gacgcggtga tcgcccgcgg
     9541 cgtcctggat cttcccgctg cggtcgccga ggtctcaggg gaacgcccca tcgatgtggt
     9601 ggccgacgtg gttgggggag actctttccc gcatctgctg aacatcctca gatccgaggg
     9661 ccgatacgtg gtcgcgggcg ccatcgccgg tccttccgtg cgcctggatt tgaggactgt
     9721 ttatctcaaa catctagagt tgatcggctc gaccctcggc gcccgcgaag acttcgccga
     9781 cctcgtccgc tacgtgagcg gcggtaaggt gaagccgctc ctggccggcg tctatccgct
     9841 ctccgagata aggcgagcgc agagcgactt ccagggtaag ggcttcttcg gcaagctggt
     9901 gctcgtgccg gtctaatgcg ccggggcatc cgttcgggaa gtccgcctgt tccaccaccg
     9961 acgcggcgca ccaggatagc cgattaaacg gcgaagtgtg caagaacttg tgatctcttc
    10021 gatggtctat tccactcgat acgttaaact cgtctctcta tgagagtaat tattgtcggg
    10081 gccgggttgg ccggcctcac atgcgcgaaa gtattgaacg aacgtggcgc cgaggtcgtc
    10141 gtcttcgagg cgtcggacgg cgtcggcggg cgcgtgcgga cggatgcggt ggacgggttc
    10201 ctgctcgacc ggggctttca ggtgtacttc acctcgtacc cggtggcgcg gcggcatctg
    10261 gaccacgacg ctctggactt ccgggtcttc gatcccggag cggtcatcca gcggggccgc
    10321 gagcggagcg tcctctcgga ccctctgcgc gacccgaagg cgctcgtgcc gtctctgctc
    10381 tcgggcgcgg cgaacttcga ggacaaggcg aggatcgtgg cgctggcggc gaaggtgctg
    10441 accggaaccg cggccagcgc gggcgaggag aacggcgaga gcgactccac gacgctggac
    10501 tatctgcgga agagcggttt ctcggagcgg ttcgtggact cgttcttcag gcccttctat
    10561 ggaggtgtgc tgctgaaccg ctcgctgact acgagcgccc gcgtcttccg gtttacgctg
    10621 cggatgctcg cgacggggag caccgtagta ccggcgcggg ggatggccgg gatcccaaac
    10681 cagctcgcct cccacctgcc gccggacgct atccacctga acagccccgt agagtccctg
    10741 ctgcgcgagg acggacgggt aaccggggtt cggaccaacg gcgaggagca cgaggcggac
    10801 gccgttgtcg tcgccaccga cgcgccggcg gcgggcaagc ttaccggaga agcggtcccg
    10861 gaagactccg tcggggaggt gtgcatctat tacgagacga gcgggatggg gagtggaaag
    10921 aagatcttat tgaacgccag cgaagacgca ttcgtgaaca acgccgtgga gatcagcaac
    10981 gtctccgagc actacgctcc gcggggccgg cgcctgctct acgccgtcac gctcaccggc
    11041 ttcgacctac ccgacgccca gctctaccgc cgggggatag aggacctgag cgactggcgc
    11101 ccggaggcga cgttcgaccc catcgccatc cggcgcatcc cctacggtca gttcgcccag
    11161 ccgcccggca tccacgaaaa gctgccggag aaccgcaccg cgacgccggg actcgtgctc
    11221 gcgggcgaat acactgagga ctcctcgata aacggctcca tgctttccgg tgagagagcc
    11281 gcgaaggagg ttctcggcgt atgacggaca tcacaccggc gctcaggata gagaagctca
    11341 cgaagaccta ctcgaccggt ctgcgcgcgc tcgacggcgt ctcgctggag atagagtccg
    11401 gcaagttctt cgggctgctc gggccgaacg gggcgggcaa gactaccctc ataaacagcg
    11461 tcgtcagcct ggcgcgtccc gactccggga gcgtggaggt gtacgggaag gacgccttcc
    11521 gggagttccg ggaggcgcgg cgtatgatcg gggtctcgcc gcaggagata aacctggaca
    11581 agttcctgac cgtcgaggag gcgctgctct tccacgccgg ctactacggc gtcccgaagg
    11641 cgaaggcgaa ggagcgggcg aacgagctgc tggagcgctt cgggctgacc ggcaagcgcg
    11701 accagcgaac aaacacgctc tccggaggca tgaagcgccg ggtgctcttc gcgcgggcgc
    11761 tgatgcacga cccgaagata ctcttcctcg acgagccgac ggccggggtg gacgtggagc
    11821 tgcgctacaa gctgtgggag tacatccggg agttgaaccg cggcggtctc accatcctgc
    11881 tcacgacgca ctacctggaa gaggccgagg ctctgtgcga ggagatagcg ctcataaacg
    11941 gcggcaggat cgccgcgcag ggtacgagcg gggacctcaa ggagcactac ggggcgagga
    12001 acatcgaaga ggtctacatg aaagtgatcg gccatgatac tgacggatag gacattccgc
    12061 acgctgctta accgggagat cctgcgcttc acccgcgtct ggatgcagac catcgtgccg
    12121 cagcttttga cctcgctact ttacctggtc gtcttcgggg tggctctcgg cgggcgcata
    12181 aaggagatac acggcatccc gtacctggag tacatcctgc cggggatcgc gctgatgagc
    12241 ctgatcacga actcccagat gaactcctcg tggtcggtct tcgacgccaa gcgagagcgc
    12301 tacatagacg aggtcctgat cagcccgatg agcgatttgc aaattactct ggcttactct
    12361 ttgggcggta tgctgcgcgg cgtcgtgatg ggcgccggcg tcttcctggt cggcatcccg
    12421 ttcgtggggc tgcacatcga gcatccgctg ctcctggtcc tgatagggct tctcgcctcg
    12481 ttcatcttct cctcgttcgg gacgatggcc ggagcgctcg cgacgaggat agaacacatc
    12541 tcgttcctca ccagcgtcgt aatccagccg ctggcgttcc tgggcggggt cttctactcg
    12601 gtgcagatgt tgccgcagtc cctccagttc gtgacgctcc tcaacccgat cttccatacg
    12661 gtagacgcca cccgctacgc gacgctcggc atctccgaca tcaacccgta tccgactata
    12721 gcaatagtct tccttctcgc cgcactatcc ttcggcgggg cgtggtgggc catacaccgg
    12781 ggtccgaacc tccgctacta gcccgcaaga ccggagggag gcccggcgga tacccggaat
    12841 ccgtagcaag gacggacggg gacgtgtgac cttcggagcg ccggctccgt atagcccaaa
    12901 ctgtagagaa aaatgtcctc tgttctaccg atgcggcgga tggtaaacga tgggatcatg
    12961 ggccgtatga aggaggagcg ggagaagatc gacgattcgg ctgtcgctga tagcggtggt
    13021 ggcactctgg tactggtggc cgtccagccg cgagcctact cccatgccat agcagcagtc
    13081 atcgaggata tgagaccccg cctgagggtg agggtggccg agccggacga cctcgaagac
    13141 caggtccggc gcctcgaccc ggacctggtg ctgtgcagcc agcccaacac ttttactccg
    13201 aacgggagac ctttctgggt tgagttctat ccatatgccg agtcgcccga cgatgagatc
    13261 cgcgtcaacg ggaagggttc ccggcagagg gccatcgagt tggcggacct gctggcgctc
    13321 ctggacggcg cgctagcccc ggatgccgcg ctgtccgaag ggtagccgtg ccctatctgt
    13381 tgtttgcact cgtggcgctg gtggtcgtgg cgctggcggt gatcgcgatc tacctgctga
    13441 tccaaccctg gctgtaagct cacggcaaaa acgcctgacg gcatgggcgg atgtatgggt
    13501 ccacaccttt ccggcaccgc aaatccgtta caatccagcg catgaggatc gcttcttttc
    13561 ctggtttgtg tcggaccagc gccggggctg cctccgcgcc tgtcgctgcg cctgccaccg
    13621 cgcgtttcgg gcgggtcgga gacttcctgc gaagcagggg cgctgtatga gccggaccgg
    13681 gcctgtgggc cggacctccg ctaccagggg gactcgcgcg cgggcttcca gcgccgccgg
    13741 tgactggccg ttcgtgctgg cggtcttcgc ctcttcgcgg gcctttttcc tgctggcggg
    13801 cgctctcgcg gcgtacttcc tgccgtgggc cgagccggcc gggtctcctc tggagccgcc
    13861 cggtcttctc aactactggg cgcactggga cggggcctgg tactcggaga tagcgacgga
    13921 cggctacgat gcccgctacc cggcctcgac cgcgttcttc ccggcgtatc cgatgctgct
    13981 gcggctcggg tctgagcttg gcggcgggcc ggcgttgtgg ggcgtcctga tctcgctggg
    14041 cgccaccctc ctctcgctct acttcctcta cagcatcgcc gagaagctct acgataacca
    14101 cacagcccgc gccgcaaccc tcgccttcgc gttcttcccg acggccttct tcctcaacgc
    14161 cgtctacacc gagtcgctct tcctggcgct gacgaccggc acgatctggg cggcttacgt
    14221 ccggcgcgac ctgctgctgg cgggcatcct gggcgctctg gccgcggcca cccgcaacct
    14281 cggcgtactg ctcctgatcc cgctcttcct ggagtggcgg cgcaaccgcc gggagttcgg
    14341 cacgtcggga ctcgtggcgc tcgggctggt gcccgtgggt cttctggcct atacggtctt
    14401 tctggcgggg aggttcgggg acccgttcgt cttcgtgagg cagcagggcg agtactgggg
    14461 acgcacgctc acgagcccgc tcgtcaccgc gcagcatgcc tgggaagccg ccggagaggg
    14521 ggccaggtac atactccacc cggcgacgct cttcctgggc gacccgtccg ctccgtcgct
    14581 ccaggcgtcg aacaccgtca gcctcgtctt cctgatcggc tttgtagcgc ttatggtgat
    14641 agggctctcg gtgctgccgc cggggctctc tatctacgcg ctcatcatca cgcttctgcc
    14701 ggtcctgacg ccgacgccgt ctttcccgct gatgagcatg ccgcgcttcg tgctgggagc
    14761 attcccgatc ttcctggtta tcggatatct cctggcgcgc cgtccgaccg ctctgatggt
    14821 atggctggtc acgagcgcgt tggtgggtgt cgcgctcacc gcgctcttca ccacctggcg
    14881 ctgggtggcg tagcgcgagc gggggttatc cacgctcctt tacgtcgcgg atgtggatga
    14941 caccgccctg gcgaccgtcc tcgtcgtcgc ggagcggccc cccggttgct tcgaaccggc
    15001 gccgcgcgcc gtcccgtgtc tcggccagga actccatgtt gaacagctct tctcgcgtgg
    15061 cccggtgacg ggggacttcg tcgggtggca ggggttcgcc gctctcgtcc aggcacacca
    15121 gaccgcgctc tccgaacatt cgctggtagg ccgcattggc gaaagcgtcc tgaccggcgg
    15181 cgtccaccac cagcacggcg gtgtccatgc tgtccaggat cgcctcaagc cgcgcctgtt
    15241 ctctctcgct gtcccgcgcg agtgattgca actccatgct ccgggcctga aggtcgtcgg
    15301 tggtggtgtt gagctcctcg atggtcgcct gaagctcctc gttgagggtt tcgagctctt
    15361 cgttggtcga ctggagctcc tcgtttaggg tctcgacctc ctcggtagcg gcctgcgcct
    15421 cttcggtgga gagcaacaac tcttcgttgg cggtctggag gtcttcgttg accctggtca
    15481 gttcctggtt gccctcgtcg agctggcgat tggtctgggc cagccgctcg ttgagtgatt
    15541 tctcctgttc cagctcggcg gtgacggact gtagccgctg cgcgagatcg ctccgggtcc
    15601 tcgccaggtt cgtgatgtcc tggatgaaga tcattacggt cttcgacggc tcccgcgcgt
    15661 cagaggtgct ctgggctcgg atgaccatcc gcaggtagcg aaattcgccg gtatccggct
    15721 cctcgaccgc gagttcgtcg acttcgacgg ggtcgtcctc ccggaaagcc gcgtcgatgg
    15781 ccgagcggag cgttgtgtac gccgcgccct ggaccagatg gatcaggtcc tccccggtcg
    15841 ccgggctgtg gatggagagc aaccggcggg ccacgttgtt gatgaactgg atgtcgtagc
    15901 gccggtccac cgccaccatg cccaccggca acttgagcag caggttgttc aagagccggt
    15961 ccgcctgctg ttgatctctt ccggcctgtt gcacgggtcc cggaatcctg gccgtatttt
    16021 cgccgggaga cgggcttttc ttcggagaga tcggtacggg actctccagg agggcgggcg
    16081 gcatcaggaa gcggtcgccg cggcggcggt agattttccg ttgccggtcc tcgggaacga
    16141 agaattcact caacggggag gttgactccg ccttgcccag caccaggcgt ccgtcgtcgc
    16201 gcagggagta ggcgaatagc tggagcgccc gcttttgcag ggccgtcgtg aagtagatca
    16261 gcacgttgcg gcagatgacc atgtcggtgt tggggaacgg ggcgcgctgg gcgaggtcgt
    16321 gctggccgaa gatgaccata ccccggatca gcttgttgac ctgatagtcg ccgtcgtcct
    16381 tgacgaagta gcggttgatc aggtcttccg acacgcctgc cagcgccgag tgtgagtaga
    16441 tcccgcgccg cgcgaaggcg atagcctcct cgtctatgtc cgtggcgaag atacgcacct
    16501 tgaaccgccc caactcgtcg cccagagcct cggccatcag gatggcgagc gagtaagcct
    16561 cctctccggt ggcgcacccc gccgaccaca ggcgtagctg gtttcccctg ttgcgggact
    16621 cctctatcag gcgcggtagc atctccgtct tcaagtggtc gaacagctcg gcgtcgcgga
    16681 agaactcggt gacgttgatc aggaaggagc ttacgagctg ctggtactct tcggggttct
    16741 cctccaggta ctcgttgtac ccttcgatgt tccccgagcc ggtggcgact atccggcgtt
    16801 gcagccggcg catgatcgtc ggcgtcttgt agctggagaa gtctatgccg ttgcgctcgc
    16861 gcacttcttc gagcagcgta cgcagcgcct cctgctctcc cggttccgac ggcgccctga
    16921 cgctcgccag caagtcctga aggatcggcc ctatcctgtc cagctccgag atgatgtcca
    16981 ccgtgttggg agcgagcgag aggggcatcc cagggtattg ggcggtcctg ggattctgga
    17041 ttatcaccgt gccgccggcc ttcttgacgg cggccgcgcc tgccgccccg tcggagccgg
    17101 tgccggtgag gatgatggcg atcaaacgct cccctataac ctcggcggcg ctgccgagca
    17161 gcaggtctat ggagggcttg ggccggccgg acgggtccac tctaaggtct atctcggagt
    17221 cggtgacgtt tacgtgacgg ttggccggca ccacgaagac cacgcccgcc tccagcggtt
    17281 cgtgatccgt caccgtccgc accggcaggg tgctcttgcg ggccaggatc tcgcccaggt
    17341 gactctcccg gtgggggtcc aggtgctggg cgatcaccac cggagcggag aagtccgggg
    17401 gaagggtcga gaccagcacc gacagggcct cgaccccgcc ggcggaggag ccgaccacga
    17461 cgagatgcga atatgttcgg ctctcagtca aggctacacc tccgatggta caaagccggc
    17521 ccgcgcaaaa gcagcttaca gggcactgcc cggaccatca aacgcgattg gatctccacc
    17581 tcagtattcg ctctccacat ccgacatcgt ccccgcggcc tttttcagag cggtaacgtc
    17641 ccggacgtgt atgtagcggt gcctggtggt caacactccc gagaccctga gttgcgtcat
    17701 ggcgttggtc acggcctcgc ggttcgcccc gatcatggtc cccagttggt agtgggtgta
    17761 gcgggtcgaa attcttatct ccccgccaac gagcgcgccc tcgctctcga cgagccggag
    17821 gatgaggctc gccaggcgcg cgtggacctc cttgatgcca atgtccgcca gccggccgcc
    17881 atactgggcc actcgctcac tcaacaggtc caccatcctc agacctacct cgggccgcct
    17941 cagaaccgtc ttgtcgaaga tgtcccggct catgagggag atcctcgacg gttcgagagc
    18001 ctgcgcgtat gcgccctgga cccgccggtc catgagcctc atctcgccga aaagtccccc
    18061 ggtcctgacc accagaaggg taaactcgcg tccttctgtt acttcgaaca gccgcacctg
    18121 cccttcgaga agcaggaaga gcatcccgct ccggtacttc ggagggtata gagtctggcc
    18181 cttgccgagg cgaacttccg gcatcctccg ggcgaggtca cttatctcat cccacgatag
    18241 aggctgcagt atgcttatta gcgagagaag ccgcgccttg tcctctatgg gcccctcctc
    18301 cggctgctca ttctgcaaca acgaaatagc cctctatttc tctccgttaa ctcagcctaa
    18361 ataatttttt tgcctaccta aaagtctaac atcctgctag taatatcaca ataaccatga
    18421 agccttctaa tgcccaccaa catcgtgagg ctcgcgcccg ggttctctat tgatcttatt
    18481 gcgttccgtt caggttgttg aagatttgtg aaatgtatca cacgataccg caattcgctt
    18541 ccgcgtgttc gacaatccgt tatgtcgatc ctctcaggga cctgataggc ccggagaagg
    18601 gcaggggaaa gggagagagg atcgacgatg ggttggcgtc tcggatcatc cccctcccat
    18661 gcggggtgct ggcccatgta tacctgggta gcgcggcgga gggggaaaga gaggctaaat
    18721 cgtggtccac aacggtggag gaaacggggc gtccggtgta ttggccgagg cggttcagaa
    18781 ttcttacgag gcattcgtgg acggcgtcgc cgccgcccag gcacggaaca tgaattttgc
    18841 gttggggtgg atcggaagct cgaccgagat gctcaaggct caggccgaga tcaacctgcg
    18901 cgtgatggag gccgtcgcgg agcagtcgcg gaggcaacgg gaggtgtcgc ggactctggt
    18961 cgaggaatcg gtcaggacct tcgtggactt tgcctatggg cctcttcccg gcaccgccga
    19021 gccagaagct ggcggagacg agaacctgcc gatagacgat ttcgaccggc tgaccgtcga
    19081 ggagataggt tccagactaa gaggtctcga cgctcgcgag gtagaggaga tcaagaccta
    19141 cgaaaagcgg cacaagaacc gcgcgatgct catggaacgg ttggagcgcg ccctgacctg
    19201 aacgggacac aggtagcgaa aggcgaggcc ggacgcgagc gagtgcgacc ttcaggtgcg
    19261 gcaggccgct cccgaggagg tggagatttc atgctttcgg aagaggtgcg gatcgggatg
    19321 agggtcaagg tatcgagcgc tcatataagg tcggacctgc gagggctgac cggcgtcgta
    19381 gagcagatgt acgggcatcc cgcttacagg gcccttgagg tgcggctgga gaacgggaga
    19441 tgcgagttat tctggcatta cgaactgaag cgtgagaacg ccttcgtatg atccccgtga
    19501 tagcagtcgc cggcgccgga cgcttacatt cgcggaggag atgagagacg tgacgcgccc
    19561 agagcgggcg ccgtactggc tgatcgcgga ctgcgacggt ggtcactttg tcccgttcac
    19621 ggtcatgtcc gacggcggcg gggagatgct gccggtcttc ggccacagag acgaagccgg
    19681 gaggttcctg cggctagcgg ctgcggaagg cgcgtgggag gtcagggaga ccggggccgg
    19741 ggagctcgtc tcgatcctca gtagcctctg cgggcatgtc aagagggtcg tcctggaccc
    19801 ctcgcccgaa gaaggtatcg aggtgtggac cgagctcgca agcctgggac gcagaagctt
    19861 tatcgagtcc ctgttggaga gggaagagtc cgcggcatct tagctgatgg aaggtgatag
    19921 agacgaagaa cattttgctt gtggatgatc acggcagttt ccgaaacgcc ctcgcgcggg
    19981 tgctcgactg gcacccggac ttccgggtag tgaaccaggc cgggacgctg gccgaggccc
    20041 gcaggcttgc cgcgcaggac gggttcgata tagcgatcat agacctcttc ctgcccgatg
    20101 gcagcgggat ggacctgatc cgggagttgc ggcaagcgaa acggcgcgcc gggctgttgc
    20161 tgctgaccat aaacctgaac tatgctcagc atgcccaggc gatagaagat ggcgccgacg
    20221 gtgtgctcga aaaatcagtg tccgtagccg agatcatcgg cgccgtggag cgcctcgtgg
    20281 acaagaaatc gagcgagcga acgctccgtg aagcgtgagg gcgttgttta tctctgacgg
    20341 cggccttcca ggacgagcag cgctatcagg gcgacctcga tcagcttgtc cgtgtagccg
    20401 atgggcgtcc gcaacccgat cagtacccac aggaagatcg tcagcgccgc gtagccgatg
    20461 agggcccagc gcgcggcgtt gtggtaacgg gcgagctgcg gtaggggcag gtagagcgcg
    20521 ccgaggagcc ccaggtatcc gagaccgttc aggatgaaga tcgggaaccc ttgaagaccg
    20581 aggtagaggt ggatcagggc ggtcgcggcg gtgaggacga tcacgccgat ctggactccg
    20641 cttatggcgg atgcgcgggg ggcggtggtc atggcgggtt acgctccttc ctgtcggttt
    20701 acacggtctg ctggacggct ttttcggacg gcgccgcgag cgacttcgcc gcgacggtga
    20761 gcgcgagggc tgcgagcgcg ccccccatca ggtacggggc cgggacgccg acgtagtcga
    20821 agacgaggcc cgccagcgcg ggaccgagga tgagggcgag gctcaggatg gcctgcacgc
    20881 cgcccatgag acggccctgc tcgtagtccg aggcgcgatt cgagacgata ctggtgacgc
    20941 tcgggatggc aagccccgct cccagagcga gcagaccgac gaccggatag agcaggaatc
    21001 ctgccgggat gagcgccata agcgcgaggt tcagcgccat gagcgcgaga ccgccgatca
    21061 gtagccgctc ctccccgagc cagggttgca ggcgtcctat cagggctccc tgcgtcacca
    21121 ccgcgcacac gccgacgaag gcgaagaaga aagcgttctc cgccgctccc cactcgaagc
    21181 gggcgctgct gaagagcggg aagttgctca ccagcccggc gaacgagagg ttgaggagca
    21241 gtatcgccgc cagcaacgcc cggatgccgg gcatcttgag cacgcccgag agctgcgata
    21301 ccgggttcaa ccggaggagc ggcaccgacc ggcgaagccg cggcggatgg gattcgggga
    21361 gcgcgaggta gccgaagacc gcgttggaga acgccagcgc ggaggccgcg aaggccgggg
    21421 cgtgcagcga gtggaagctc aggagcccgc cgagcgcggg accggccatc agcccgagcc
    21481 cgaaagccgc cccgatgagg ccgagaccgc gggcgcggtc gtctttcgtg gtgctgtcgg
    21541 cgatgtacgc ctgcgcggta gctagagttc cgctcgcgcc gccggcgagt acgaccgcca
    21601 ggacgagagc ggacagagat cccgcaagcc ccagcagcag gtatgcgagc gaagccccga
    21661 gcaggcacat cagaagcacg ggacgccttc cgatgcggtc cgagagtccg ccgaggaagg
    21721 gtccgccggc gaactgcatc atggcgtaca gggaacccag gagccccacc agcagggctc
    21781 cttccgcgta ctggcgggcg aagaagggca ggagcgggac gacgataccg tacccgatca
    21841 tgtccacgaa caccgtgacg aacaggaaga ccagcggagt cctccggtct ccggcgagcg
    21901 gcactagcgg gctccgcccg actcgatcag acgccgcatc tccattcgca ccgcgcctcc
    21961 gacggcttct ttcttgccgg cgacgatggg gttgccgaac gcgacccgca cggtaacgcc
    22021 ccgcagggcg ggggtgagca tctggagcgt ggcggcgagc cattcctggt cgctcctgcg
    22081 gcggcgcagg cgggtcaggg ggtttcggag cgcggcaggc gagatgacgc cgctcacggc
    22141 gacggggacg atggcgaggc ccggcgtgag cttcgcgaac aggtccacgc tcgccgacca
    22201 gctcccgagc gactctatag cgccggggag tatggccgga tctggctcga tcctgcctcc
    22261 cggaaacgtg aggaccgcgc cgccatcgcg cagatgcctg gtcgccgccc gtaccggccc
    22321 gaaacgcccc ggagacgtct ccgcgacggt gatcaggtgc cgggaagtgt ttggcagcgc
    22381 ccccagaaac ggccgctcgg cggcgatcac gcgcagatcc gggcgcggcg tcgcggcgaa
    22441 cagggcgacg gcgtcggcga gccccggatg gtttgagacc acgagtagcg gtccctccag
    22501 cggcacgctc tccgcgcctt cgacctccgc gcgccgggcc aactgcttga gcgcccacgc
    22561 cccgcccgcg ccgaggccgg agtcgcccac gatcgcgtcg taggtcagga cctgacgggc
    22621 cagccggcgg gccggtatcc tggctacggc ctcaagcgcc gcgcggcttc gacgcttctc
    22681 tctcaacccg aacgcggaga acaggtcatc cacacagacc cgcgtcaggg tgtcgagatg
    22741 ctcctgcctc tcgggcacgg gcaggcggaa cggtccggcg ggtaatccga atacgttatc
    22801 caatgctctc catcctgccc gtatctctca tctacgcacc cagtatggcg gcggccccgc
    22861 ggctgctcat cgctaaaact aaccttattt tcctggccca tttgagctag agcctttagc
    22921 tagagccgtc ggtcagagcc gggcccgcac cgcgcagcca catcgtaggt tcttacgcac
    22981 ggcgcgccgg ggtggtttga gggggtggcg gattctcgcg cggagaggtt ggttactctg
    23041 aggccgcggt ctcgcggcgg cgcaggacct cgacggcgga accggagagg accgtgccgt
    23101 ccagccggac gtgcggcttg gtgaccttta ccttcacggc ctcgaccagc gggaagttct
    23161 ccatgacggc cgcggcgatc ctctcggcga gcgtctcggt gaggccgacg ggctcgccct
    23221 cgacgatagc gcgcgacatg cggtgtactt cgctgtagtc aacggtcttc gtgaggtcgt
    23281 cggaggagcc ggcggggcga aggtccaggc gcatctcgat atccacgacg aacggctggc
    23341 ccagctcgcg ttcggcgggc agcgtgccgt ggcggccggt gaagaccatg ccttctagca
    23401 gtatccggtc ctcgctcatg ctctcagtat gacagcatcc ggagcctcgc gtcaccctga
    23461 cggtcccctc gcgcggcatt gctggcgcca gccgtgttaa ccttcttgag tcagggaacc
    23521 ggcagggaga gtcatcacgt atccggagat cacggtcagc tacgctcaga cgttggacgg
    23581 gaggcttgcg acggctggtg gaagctcgca atggataagc tgccgcgact ctctgcgctt
    23641 cgcccacgcg ttgcgcgccg cccacgacgc catcatcgtc ggggcgggga ccgcctgcaa
    23701 ggacgacccg cgcctgaccg tccggctcgt tcctggagac gatccgctgc gcgtcgtggt
    23761 tgacagcacc ctccgcacgc ccctcgccgc cgccgtgctg gcggacgggg ccgcgaaggg
    23821 gactgtgatg gccgtgaccg ggcgggcgcc ggaggatcgc tgcgaggccg cgcgggctct
    23881 cggcgcgacc gtgttgcggc tgccggagga tggggccggc agggtggacc tgcgcgcgct
    23941 gctctcggag ctgcgggaga tgggggtaga gtccgcgatg gtcgagggcg gggcggcgct
    24001 catcacggga tttttccgcg agcggctcgt tcggcggctc gcggtgtgca tcgcgcccaa
    24061 gatcctgggc tccggcatag aggccgtggg ggacctgggg gtccgggacc tggccgactc
    24121 cctgacgctc accgggacca ccatccagtc ctgtggagtg gacctgatcc tggacagccg
    24181 cgtcgagtat ccgaacggta gcccgaacgg tagcccgaac ggtaacggag ccggtgccag
    24241 aggggcttga ggcccgcgcc ctctggttcg ccgcgccgcg aaccgcggag ttgcgctccg
    24301 agaacgcccc cgcgcccggt cccggagagg ttcgcgtccg agcgctcgcc tcggcgataa
    24361 gcggcggcac cgagatgctc gtctaccgcg gcgaggtccc ggaaggcatg cagctcgacc
    24421 tccccacact cgccgggagc tacggattcc ccataaagta cggctacgcc gccgtggggc
    24481 acgtcctgga tatcggctct cacgtgcagc gcctctctcc cggagacccg gtcttcgtcc
    24541 accacccgca ccaggacttc tttaccgtcc cggcccacac gccggtccgg ctccccgaag
    24601 gtctcgaccc gcttctcggc gtctactgcg ccaacctgga gacggccctg aacatcgttc
    24661 acgatacgcc cctgcgcctc ggtgagacgg cgctcgtatt ggggcaaggg gtggtcggcc
    24721 tgctcgtgac gcagctattg aagatctccg gcgccggaga ggtgctggtc gtggacccgt
    24781 ttcggaagcg gcgcgacctg gcactcgccg tcggcgcgga cgcggccttc aagccggacg
    24841 aagaactggg aaccaggctg cgagagatga ctggcggacg cggcgcggac gtggtggtcg
    24901 aggtgagcgg gtccggggag gcgttgcagg ccgggatcga gcacgtcgcg gatgagggga
    24961 ccgtggtggt cgcgtcgtgg tacggggcga agcccgtctc gctctcgctc ggcggacact
    25021 tccatcgggg gagggtcaag atacgttcct cgcaggtcgg gcggatctcg ccggagctgg
    25081 ggccgcgctg ggaccgcgac cgtcgtatgg tagccgtcct gaagctcctt ccccggctac
    25141 ggctggaagg gttgatctct caccgcatcc cgttcgagaa ggcgccggag gcttaccggc
    25201 tcctcgacga aaatcccgaa gaagtcgtcc aggtgatcct gacctacgag cgctcctgaa
    25261 gcgggaccat cgaacgggag cagctcccag ctctccgcac gctttccggt ccaacgggat
    25321 acttccgcca tctcggctgc gtgtcgcggg agcgcgatct cgcgcgcttc gttatccttg
    25381 tggtaccagc tcaaggagga agcaggatgt atgagatcta cgtggcggcc cggttcgagg
    25441 cggcgcaccg gcttatcggg gacttcggac cggcggcccg gacgcacggc cacacctacc
    25501 ggatggaagt catcgtgcgc ggcgagcgcc tgaaggaaga cgagaccctc tacgacatcg
    25561 gcgagctgcg tccggcggtg gacggcctcg ccgcctcgct ccactaccgc gacctgaacg
    25621 aggtgccggg gctggaggac gtgaacacca ccgccgaggc cgtcgcccgc tactgctggg
    25681 agaagctcgc cccacccctg cgcggtaacg ggctcgactc gctcgcggta cgcatctggg
    25741 aatcatccga cgtctacgcc gcccgcgagg actcgctggg atagaccttg cgcgtcgcct
    25801 tcgtcaccgt gggggacacg agccgcatga ccggcggata cctatacaac gcccgcgtca
    25861 tggacggtct gcgcgaacgt ggagtacggg tggaggagat agtggcctcc agcgcttccc
    25921 cggaggagca gcggaccgcc gcgcggcgcg gggatgctct ggaccccacg gacttcgatg
    25981 tagtcgtggt ggacgctctg gcgcgtatcg cggtagcccc gcatctcgac cgctggcgga
    26041 tggagtgtcc ggtagtggcg atggtacacg agttgccgag catcgcgggc ggcgagtccg
    26101 gcgccgaggc cgccgcccgt gagcgggagt acgaggagcc tctgctgcgc tcggatctct
    26161 ttatagccgt gagcgatcac ggccggaggt tgctggagat ccagggcgtc cctcacccgc
    26221 gcgtctacat cgtgcctccc ggcttcgacc gcctgcccgc gcccgcggag cccgtagaca
    26281 acctcgacgg taccgtgcgc gccctgtgcg tcgcgcagtg gataccgcgc aagggcatac
    26341 tgaccctcgt ggaagcctgg acccggcgag agcgtccggg agcggcgctg gagcttatag
    26401 gagagacgga cgccgacccg gagtacaccg cacaggtcag agagaccatc gcagccgcgc
    26461 ctgattcatc cctcgtcgtg tctgggactg tggatgatgc tgaactaatg agatcctacg
    26521 cggcggcgga cctcttcgcg ctgccctcgc gctacgaggg gtacggcgtg gtctacgcgg
    26581 aggcgctctc tttcggattg ccggtggttg cctgcgaggt ggggccggtc cccgaactag
    26641 tcggcaggga cgcggcggta ctggtcccgc cggacgaccc cgtcgcgctc gccgaagcac
    26701 tcgacctttt gctcgaagac ccggatctcc gccgccggat gtccggcgcc gcccgccgcc
    26761 gcgcggaacg tctgccgcgc tgggaagata ccgtgtccgg cttcgagacg gcattgcggg
    26821 cggcggtccg ggacggggcg cgccgaaccg gcgcgagata gaatcccgcc gtggaccgca
    26881 gagacctgcg cgagcagaac cggctctcct ggaacgccgt ggtcggggcg cacgacagcc
    26941 accgcgggga tctcgtgggg ttcttccgcg gcggcggcaa caccctcttt cccgaggagc
    27001 gcgagcttct gggcgacctc gaaggcaaga ctctcgcgca tctccagtgc aacactggcg
    27061 gagattcgat cagcctggcg aagctcggcg ccgtagttac cggcgtggac atcagcgacg
    27121 aggccgtact ctcggcccgc gaactatccg aaaagaccgg catcccggcc gccttcgagc
    27181 gcgcggatct atacgactgg ctcgaagagt cggcgcgggg aggacaccgg ttcgacgtcg
    27241 tctacgcctc ctacggcgtc gtctgctggc tcccggacct cgcaggatgg gccgccggta
    27301 tagccgccat cctggaacct ggcgggcgct tcgtgctcgt ggacttccat ccggtagccg
    27361 acatgttcga cgaggactgg aaccacgctc gcggctaccc gcatggcggc gagccgctac
    27421 cgctcggaga aggcgtcgga gactacgtag ccgaatccgg gggtgggctg actccggcgg
    27481 gctttgccga aggtgtccga agcttccaga acccggaacc cgcgcacctc tttcgttggg
    27541 gactcggaga agtcgttacg gcgctggcgg gggcgggatt gaggatcacg gctctcaaag
    27601 agtaccctta ttcaaacggc gagcgccact tcgcccggat gcgcgagctg aaagggaggc
    27661 gcatggtccc gccggaagac gttcccgccg tacccttgat gtacggtatt cgcgccgaca
    27721 agctcggcta tggcgagaag aagaacgcca gcgcggcgat gacgtagacg ccgcagagca
    27781 tcgcgccttc gagccag
//
LOCUS       DASSMB010000221        16135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_694728, whole genome shotgun sequence.
ACCESSION   DASSMB010000221 DASSMB010000000
VERSION     DASSMB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16135
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694728"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VFJ72_13145"
     CDS             <1..1023
                     /locus_tag="VFJ72_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HET7480451.1"
                     /translation="IAKGARVAQTVTLIPGDGIGPDLTDSVKEVIGALEVDIEWEIAE
                     AGETVMDREGTPLPESVLESIRRNKVALKGPLTTPVGTGFRSVNVALRKELDLYGNIR
                     PSLSLPGVETPYKDIDLIIYRENTEDLYAGVEHMVGKYAAESIKIITREGTERICRMA
                     FERSKEQGRKHITVIHKANIMKYTDGLFRDVFFEVGKEYENDFEEIDDRIVDNMCMQL
                     VMKPNLYDVLVCPNLYGDILSDLCAGLTGGLGVAPGANIGEEIAVFEPVHGSAPKYAG
                     QNRANPLATLLSAKNMLIHMGYEDEAERMLGGIEAALKSPETRTKDLGGSAGTKEFTQ
                     AIISNL"
     gene            1050..2171
                     /locus_tag="VFJ72_13150"
     CDS             1050..2171
                     /locus_tag="VFJ72_13150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET7480452.1"
                     /translation="MRIAFFTETFLPATDGVVTRLKHTIEELQRAGDEMLVIAPKYGE
                     GPGSYLGVPIHRVPSVPFPPYPQIKLAPVNRAVGRALRKFSPDLIHAVNPFILGPGGL
                     FYAKRLNVPLVASYHTNIATYADYYNLGFARRGTAWYTRNLHNRADLNLCTSQATFRY
                     LASERVERLRLWPQGVDAERFHPDKFSKHWRVRLTGGHPEDHLLLYVGRLAKEKGIER
                     LKDVLAEMPGVRLALVGDGPGRRDLEKVFAGTPTVFTGTRKGQELSAVYASADALVFP
                     STTETLGLAMLESLASGLPVLAARTGAAGEVVSDGENGVLYEPRSKVSLIEAVRHVLF
                     DEELRLCLSRNARAAAEGRDWERSTQALRGYYLEALAAR"
     gene            2168..2629
                     /locus_tag="VFJ72_13155"
     CDS             2168..2629
                     /locus_tag="VFJ72_13155"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7480453.1"
                     /translation="MSKRDKLKRGGKRFSQFTLVGILNAAVDFGVLNLFLWLAPTRDP
                     WQLAAFNGVALVAANINSYWWNTRWTFRGRAEHDFRQVFLFALQALVNIGISNGLFWA
                     LIHPLLTDTDIPAYLVGNVAKLISVIVASTISFFIMRYLVFSRKRWFGGRL"
     gene            2690..2762
                     /locus_tag="VFJ72_13160"
     tRNA            2690..2762
                     /locus_tag="VFJ72_13160"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2723..2725,aa:Gly,seq:gcc)
     gene            2798..2868
                     /locus_tag="VFJ72_13165"
     tRNA            2798..2868
                     /locus_tag="VFJ72_13165"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2830..2832,aa:Cys,seq:gca)
     gene            2881..2952
                     /locus_tag="VFJ72_13170"
     tRNA            2881..2952
                     /locus_tag="VFJ72_13170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2913..2915,aa:Val,seq:gac)
     gene            3082..5007
                     /gene="thrS"
                     /locus_tag="VFJ72_13175"
     CDS             3082..5007
                     /gene="thrS"
                     /locus_tag="VFJ72_13175"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7480454.1"
                     /translation="MAAVRLPDGKELEIESGERARDVVERIGPRLARDAVVAKLNGDL
                     IDLDAPLDGGGDFEVVTKDSPEGLYVLRHSTAHAMAQAITELYPGSKLTIGPPIENGF
                     YYDIEVAGRITDEDLPRIEERMREVVQRDLPIQRQSVPKADVEAMYEDNPYKLELIQD
                     LEDGDITVYRQGDFFDLCRGPHVPTTAKLGAFKLQNIAGAYWRGDEKNTMLTRIYGTA
                     WPTEKELKAYLRRLEEARQRDHRKIGKDLHLFTFSPDIGAGIPIFLPKGEMLRHLMES
                     YVREVQTRHGYDHVWTGHLANENLYIKSGHIEHYRDDMFPPMVDEDTRYRLKPMNCPS
                     HMTLYNTQSHSYRDLPLRYAEFATLYRYEKSGQLTGLTRVRALTQDDAHIFCTEGQVQ
                     EEFARALEIIREVLDTYGFEDYRVRLSMRDSESGKFIADDEKWTRAENALRQALDAAG
                     IAYEEVPGEAAFYGPKADFMAKDVLEREWQLSTIQVDFIQPARLGCEYIGEDGQAHTP
                     VLLHRAVTGTTERFMAVLIEHYGGAFPTWLSPVQAVIIPVADRHLEYARKVQASLDAA
                     GLRVEVDEALMSMQKKIRENARQKIPYLLIVGDEEAGEGTVNVRRRGEKQQEAVAVEE
                     FARRVGEEIAARDSYRQ"
     gene            5151..5696
                     /gene="infC"
                     /locus_tag="VFJ72_13180"
                     /pseudo
     CDS             5151..5696
                     /gene="infC"
                     /locus_tag="VFJ72_13180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            5850..6038
                     /gene="rpmI"
                     /locus_tag="VFJ72_13185"
     CDS             5850..6038
                     /gene="rpmI"
                     /locus_tag="VFJ72_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET7480455.1"
                     /translation="MKTHQGAAGRFEVGKKGKIKRRRSGHNHILEKKSRKRKRRLNTE
                     TFIHSSDEKRIKRLLGVR"
     gene            6040..6867
                     /gene="rplT"
                     /locus_tag="VFJ72_13190"
     CDS             6040..6867
                     /gene="rplT"
                     /locus_tag="VFJ72_13190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET7480456.1"
                     /translation="MARTARSLHARKKRRKVLEQAKGYRGTKHSSYKRAKEQVWKSGV
                     YAYQGRKQRKRDFRALWIQRINAGVREHGLSYSQFIHGLKLAEIDLDRKILADLAATE
                     PEVFAAVVTQAKNALEGNPGESRVKVERRAEPEPRPHPSRRKSRKTEKPAHEEAPESA
                     ATEEAAQAEAPQAVADEPVAEQASAEADEDVEATEAAERKAEELDVDLESVEGTGAEG
                     RVLVEDVEQTADEQGKVVATDTAEERARELGVDLESVEGTGANGRITVEDVEKAANK"
     gene            6897..7568
                     /locus_tag="VFJ72_13195"
     CDS             6897..7568
                     /locus_tag="VFJ72_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET7480457.1"
                     /translation="MISAKRAARLNQKKRRETEKLFLAEGASFLEESPEPPVALFETT
                     EEVRRISSFTTPAGPVGVFPFVDVEPERLLQSCERVILLHGVQDPGNVGTIIRAAHAF
                     GAGVALSIGCADLYNPKTVRATMGSIFHAPVARDLDSLDFIRMADAAEFVRVAAVVET
                     GGAALDRLAGKVLIVVGAEGKGLPEEIISACDRGVTIPSRAASLNAAMAASILLYEAY
                     RRVLP"
     gene            7577..8605
                     /gene="pheS"
                     /locus_tag="VFJ72_13200"
     CDS             7577..8605
                     /gene="pheS"
                     /locus_tag="VFJ72_13200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7480458.1"
                     /translation="MTTASRIEELKSEALAAVSAAGSTADLESVRVRYLGRSAELTEI
                     KKSIGTLAPDQRKEVGGSANRASRAVEASISERLEELSVREREERLRAEAVDVTLPGV
                     PVPKGHLHPSQRIIDEVVDFFVGLGYRVAEGPEVETDYYNFTALNIPPGHPARGMQDT
                     FFLDDGLVLRTHTSPVQVRTMLSQEPPVYVVCPGRTYRRDSDPTHTPMFHQIEGLAVD
                     RGLTLAHLKGTLAAMARHVFGDRVRLRLRPSYFQFTEPSVEMDVSCFVCDGTDPDCKV
                     CKGAGWLEMGGAGMVDPAVLEEVGYDSEEYTGFAFGFGPDRMAMVKYDIPDLRLFFEG
                     DLRFLRQF"
     gene            8627..11083
                     /gene="pheT"
                     /locus_tag="VFJ72_13205"
     CDS             8627..11083
                     /gene="pheT"
                     /locus_tag="VFJ72_13205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7480459.1"
                     /translation="MRVPLSWLHEYVDFDLSVEELVDLFSLRSQEIDGVYRLGVLDGE
                     VVVGEVQEFGPHPNADRLSVAKVDLDGQVVQIVAGAPNPYPGARVPVVLPGSVMADGT
                     KLKKAKLRGLESYGMMMSERELGISGDHGGILLLDESYEVGRPVSDYFPAGEVVIDID
                     VTPNRPDLWGMIGVARELAAILQTGFRIPEVSFETTNGKPTGEYTLRVEAEDLCPRYD
                     LRRISELTPGRQAPLEMRRRIYAAGMRPIGAVVDATNYVMLETGQPIHAFDAEKVREG
                     IVVRRGREGEKITLLDGSTRTLDEEMLVIADEERGLVIAGVMGAEDAEVSEQTTDTLI
                     EVANFDGTNILRTSQRLGLRTDASGRFERGLDPNMVDYAMDRVTALLTAYPGGEAAED
                     TLSHYPEPVRKWQVPLRPERARLLLGMPVDEDDAARRLESLGCEVRPEDGRLLVTAPT
                     FRRDLRREADLIEEVGRLIGLDRVPEEIPGVPTPGGLTPEQRKLRLLRHLLADLGLAE
                     AITYPFGPDRWDRDLAPGAEASEDCIRVKNPLSAEAANLRSLLLPGLLDAAARNRTFG
                     ARGGALFEVGHVFEPSPVSEEQREVALRFRLTGAKPDADATADGLMGVSEDLRLGVVL
                     AGTTRPVGWNAPESRANYFQAKGIVERILPDAVFEASAKPFLHPGRAAVVRVGSIEVG
                     WVGELHPATIEGFDLEGWPVAAFELDVAYCDPDPEPRFTPFVNVPAVSRDLAVILRAD
                     TLVGDILGAVKRLGSEILVEERVFDVYEGPQVPEGTKSVALNFTFQGRETLTDERVED
                     EMRRISERLTQEFDARLRDS"
     gene            complement(11080..11640)
                     /locus_tag="VFJ72_13210"
     CDS             complement(11080..11640)
                     /locus_tag="VFJ72_13210"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7480460.1"
                     /translation="MRLSGRSRKLVGERVELRRHESMDYELYGRWYGDPEIWRLTSWA
                     GEPLTGSAVRRLFEDRELSTTEDSFAIHPKEGRGPVGIVSLMNISEANGTADLSIIIG
                     PPEARGRGYGTDAIRAILRYGFEERGLGRVGLSVFDFNEAAIRAYTALGFREEGRLRA
                     ALEREGKRHDAILMSILASEWREGSG"
     gene            11694..12053
                     /locus_tag="VFJ72_13215"
     CDS             11694..12053
                     /locus_tag="VFJ72_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480461.1"
                     /translation="MKVVPDRIVSLGFGKYVRADKIAALVPIEEDRGSGRRTLVYVEG
                     MKEPLVAGRTESTILRDMVGAESGAMELLRELQMQIGQVRPLLKGSIRSEAGLDLDQL
                     SRRITNLSGEQESPRIF"
     gene            12047..12655
                     /locus_tag="VFJ72_13220"
     CDS             12047..12655
                     /locus_tag="VFJ72_13220"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET7480462.1"
                     /translation="MLGTGFYDRDSREVAVDLLGCVLSHESSEGLTSGVIIETEAYRP
                     DDPACHAYKGPTMRNRTIFGAPGLAYVYLSYGVHHLINAVCEEEGTGSAVLIRALRPL
                     EGEDLMAERRGRNDHLCEGPGRLTQALGIDLSKDGHDLTDGGLTIRPGPPPEAEIVAT
                     TRIGISRGTDLPWRYLVLGDPNVSLPPRGISRRGISRSDLLD"
     gene            complement(12652..14706)
                     /locus_tag="VFJ72_13225"
     CDS             complement(12652..14706)
                     /locus_tag="VFJ72_13225"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7480463.1"
                     /translation="MFRVLGIFFLCLFVAGLAFCIGAYLGLVKSVGQLDKPQSVESHP
                     TYIYSAPLGGDDGPRRVIGTIFQGENRKTASLDEMPPNLLNALVAKEDERFREHGGVD
                     LWGIMRALWVDFRAGKKVEGASTITQQYVKNAYLNQNQTWTRKIKEALIAVEIERSTY
                     KNKDQILADYLNTVYFGSNAYGVEAAAETYFNKSVDDLTTGESAALIGILWSPSTLGS
                     DRDAATEQRDLVLKKMFDAGYLTQQDRDKALDEQLPKEWPVAPMVESGLTGPQLTRNF
                     TELVQQELIDKYGSTTVLNGGMSVYTTLDLKAQVAAQDTIYGPNGYLPSPDDPDAALV
                     SIEPKTGQIKAMVGNRDQSSHFNLVTQAQRQPGSSFKPFALIAALEQGIDPMTQFVSE
                     KKVYEVPNGSGGTERWKVENFGKKERGPISIEEALWQSDNTVFTDLAMNTDGKGLKNG
                     PESIARVANKLGITADFGKHPHPSIVLGAKEVSPLDMATAYATIANGGKRIEPTAITK
                     VVEQEGQKDEKVLFTAGKPKGKQVIDPKVAAKATEIMMGDITNGIAGRAALPDRPVAG
                     KTGTSENFFDAWFEGFTPQLATGVWMGYGEGGATLQELLDPLEGSMYGQVGSPTEIWH
                     DYMLEVMDGKPVEQFKGVDSTTAANTVNAANGTTEIQTGTSTGTVTEAPADTGTTSTT
                     QP"
     gene            14897..16063
                     /gene="tyrS"
                     /locus_tag="VFJ72_13230"
     CDS             14897..16063
                     /gene="tyrS"
                     /locus_tag="VFJ72_13230"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7480464.1"
                     /translation="MTDILNFSSIFQNAVDNVPADELGRRLRSGEALRVKLGLDPTAP
                     DIHLGHAVVLGKLREFQKLGHTAVLVVGDYTARVGDPSGRSKTRPVLTPEEIEANIRT
                     YLEQAYLILDPDQTEVRHNSEWLAPLTLADIIHLTRATTVARILERDDFSKRYAANDQ
                     ISLTELLYPLMQAYDSVAIDADVELGGTDQLYNLLMGRQIMEYYGKRPQCVLTTPLLV
                     GTEGKMKMSKSLGNYIGVSEPPNDMFGKAMSIPDGLMPEYYSLVLGRTPPDAEPVRQK
                     RELARSLVRTFHGQDAVEGAEQTFDTVVRRGVPEDVPEVALPEGDHAWIVDLVVRAGF
                     AKTNGEARRFIKGGAVRIGGEVLTDERAALPTKALSGKILQVGKRRYARFIASG"
BASE COUNT         3011 a         5065 c         5251 g         2808 t
ORIGIN      
        1 atagcgaagg gagcgcgcgt ggcgcagact gttactctca tacccggcga tggcattggc
       61 cccgatctca ccgattccgt caaagaggtg atcggggctc tagaggtaga catcgagtgg
      121 gagatcgccg aagctggcga gacggtgatg gaccgggagg ggacgcccct gcccgagtcc
      181 gttctggagt ccatccggcg caacaaggtt gccctcaagg ggccgctgac aactcccgtg
      241 gggacgggct tccgctcggt caatgtcgcg ctgcgcaagg agctggacct ctacggcaac
      301 atccggccga gcctgagctt gccgggggtg gagacgccgt acaaggacat agacctgatc
      361 atctaccgcg agaatactga ggacctctac gccggggtcg agcacatggt cggcaagtac
      421 gcggcggagt ccatcaagat catcacccgc gagggcacgg agcgcatctg ccgcatggcc
      481 ttcgagcgct ccaaggagca gggccgcaag cacattacgg tcatccacaa ggcgaacatc
      541 atgaagtaca cggacgggct cttccgggac gtgttcttcg aggtcggcaa ggagtacgag
      601 aacgacttcg aggagatcga cgaccgcatc gtggacaaca tgtgcatgca gctcgtgatg
      661 aagccgaacc tctacgacgt gctggtctgc ccaaacctct acggcgacat cctctcggac
      721 ctgtgcgccg gcctcaccgg tggcctgggc gtcgcgccgg gcgcgaacat cggggaggag
      781 atcgcggtct tcgagccggt ccacggctca gcgcccaagt acgcgggcca gaaccgcgct
      841 aacccgctcg ccacgctgct ctcggcgaag aacatgctga tccacatggg ctacgaggat
      901 gaggccgagc ggatgctcgg cggcatcgag gcggcgctta agagccccga gacgcgcacg
      961 aaggaccttg gcggcagcgc cgggaccaag gagttcacgc aggctatcat ctcgaacctc
     1021 taaccggcga cgggaccggc cgcgactact tgcgcatagc gttcttcacg gagactttcc
     1081 tcccggcgac ggatggcgtc gtcacgcgcc tgaagcacac catcgaggag ctgcagcgag
     1141 cgggagatga gatgctggtc atcgccccga agtacggcga agggccgggg tcttacctcg
     1201 gcgtgcccat acaccgggtg ccgagcgtgc cgtttccgcc gtacccgcag ataaagctcg
     1261 cgcccgtcaa ccgtgccgtc ggacgggcgc tcaggaagtt ctcgccggac ctgatccacg
     1321 ccgtcaaccc gttcatcctg gggccgggcg gcctgttcta cgcgaagcgc ctgaacgtcc
     1381 cgctcgtggc ctcgtaccac acgaacatcg ccacctacgc cgactactac aacctcggct
     1441 tcgcccgcag aggaaccgcc tggtacacgc gcaaccttca caaccgcgct gatctcaacc
     1501 tctgcacctc gcaggccacc ttcagatatc tggcgagcga gcgggtcgaa cgtcttcgcc
     1561 tttggcccca gggcgtggac gcggagcggt tccacccgga caagttctcg aagcactggc
     1621 gcgtccggct gaccggtgga cacccggagg accacctgct cctgtacgtg ggccggctgg
     1681 cgaaggagaa ggggatcgaa cgcctcaagg acgtcctggc ggagatgccc ggagtccggc
     1741 tcgcgctggt cggggacggt ccggggcggc gggacctgga gaaagtcttc gccgggacgc
     1801 cgacggtctt caccggcacc cgcaagggac aggagctgtc ggcagtgtac gcctccgccg
     1861 acgcgctggt cttcccctcg acgaccgaga cactggggct cgcgatgctg gagtctctcg
     1921 cgtccgggct gcccgtcctc gcggcccgca cgggcgccgc cggagaggtg gtcagcgacg
     1981 gtgagaacgg cgtgctctac gaaccgaggt ccaaggtctc cctgatcgaa gccgtccgcc
     2041 acgtgctgtt cgatgaggag ctccggttgt gcttgagccg caatgctcgc gccgccgccg
     2101 aagggcgcga ctgggagcgc tctacccagg cgctgcgcgg ctattacctt gaggcgctgg
     2161 cggcgcgatg agtaagcgcg acaagctcaa gcggggcggc aagcggttct cgcagttcac
     2221 gctcgtgggt atcctgaacg ccgccgtgga cttcggggtc ttgaacctgt tcctgtggct
     2281 cgctcccacg cgcgacccgt ggcagctcgc cgccttcaac ggcgtggcgc tcgtcgcggc
     2341 caacatcaac agctactggt ggaacacgcg ctggaccttc cgcgggcgcg ccgagcacga
     2401 cttccggcag gtcttcctct tcgcgcttca ggcgctcgtg aacatcggga tcagcaacgg
     2461 gttgttctgg gccctgatcc acccgctact caccgatacg gacatacccg cctatctcgt
     2521 cggcaacgtc gcaaagctca tctcggtgat cgtcgcctcc acgatcagct tcttcataat
     2581 gcgctacctg gttttctcgc ggaaacgctg gttcggggga cgcttgtaag agcggaaagc
     2641 ccgtgctaaa ttattgcggc gcgagagcgg tagctgacgc gagcgcgatg cggaagtagc
     2701 tcagctggta gagcatcacc ttgccatggt gagggtcgcg ggttcgaatc ccgtcttccg
     2761 ctctggccgc ggcgccgcag tggcaggcgg cacgactggc ggcatggccg agtggttagg
     2821 caagggtctg caaaaccctg taccccggtt cgattccggg tgccgcctct cgacggatac
     2881 gggcgattag ctcaggggga gagcgcttcc ttgacgcgga agaggtcagt ggttcgaatc
     2941 cactatcgcc cactcccctg accggcaccc ggtagacttt atccccggtt tcacttccag
     3001 ttttatttca cccccgccta gcaggtagaa tcggacatga tggtgcatgc tctgaaactc
     3061 agataagcga gaggatggtc gatggctgcg gtcaggctac ccgacggtaa agaacttgag
     3121 atagagtccg gcgagcgggc tcgggacgtg gttgagagga tcggtccgcg cctcgcccgc
     3181 gacgctgtcg tggcgaagct gaacggagac ctcatagacc tcgacgctcc cctggacggc
     3241 ggaggagact tcgaggtggt gacgaaggac tcgccggagg gcctctacgt cctgcgccac
     3301 tccacggcgc acgctatggc ccaggccatc acggagctgt atcccggcag caagctgacc
     3361 atcgggccgc cgatagagaa cggcttctac tacgacatcg aggtagccgg gcgcatcacc
     3421 gacgaggatc tgccgcgcat cgaggagagg atgcgcgagg tagtacagcg ggatctcccg
     3481 atacagcgcc agtccgtccc gaaggctgac gtcgaggcca tgtacgagga caacccgtac
     3541 aagcttgagt tgatacagga cctcgaagac ggcgacatca ccgtctaccg ccagggagac
     3601 ttcttcgacc tgtgccgggg gccgcacgtc ccgaccacgg cgaagctcgg cgcgttcaag
     3661 ctccagaaca tcgctggagc ctactggcgc ggcgacgaga agaataccat gctcactcgc
     3721 atctacggaa cggcctggcc gacggagaag gaactgaagg cttacctgcg gcgtttggaa
     3781 gaggcgcggc agcgcgacca ccgcaagatc ggcaaggacc tgcacctctt caccttctcg
     3841 ccggacatcg gtgcgggaat cccgatcttc ctgccgaagg gcgagatgct gcggcacctg
     3901 atggagagct acgtccgcga ggttcagacg cggcacggct acgaccacgt ctggaccgga
     3961 cacctggcga acgagaacct ctacatcaag tccgggcata tcgagcatta ccgcgacgac
     4021 atgttccccc cgatggtgga cgaagacacc cgctaccgtc tcaagccgat gaactgcccg
     4081 agccacatga cgctctacaa cacgcagtcc cactcgtacc gcgacctgcc gctccgctac
     4141 gccgagttcg ccacgctcta ccgctacgag aagagcggcc agctaacggg tctaacgcgg
     4201 gtgcgcgctc tcacgcagga cgacgcgcac atcttctgca ccgaggggca ggtccaggaa
     4261 gagttcgcgc gcgcgctgga gatcatccgc gaggtccttg acacctacgg tttcgaggac
     4321 taccgggtcc ggctctccat gcgcgactca gagagcggca agttcatcgc cgacgacgag
     4381 aagtggacgc gggcggagaa cgccctgcgc caggccctcg acgccgccgg aatcgcctac
     4441 gaggaggtgc ccggcgaggc agcgttctac ggacccaagg ccgacttcat ggcgaaggac
     4501 gtgctggagc gggagtggca gctctccacg atccaggtcg acttcattca gcccgcgcgg
     4561 ctgggatgcg agtacatcgg ggaggacggg caggcccaca cgccggtcct tctccaccgg
     4621 gccgtaaccg gtacgacgga gcggttcatg gcggtcctca tagagcatta cgggggtgcg
     4681 ttcccgacct ggctctcgcc ggtgcaggcc gtgatcatcc cggtcgccga ccgccacctg
     4741 gagtacgccc gcaaggtgca ggcgtcgctc gatgccgccg ggctgcgggt cgaggtagac
     4801 gaggcgctga tgagcatgca gaagaagatc cgggagaacg cccgccagaa gataccgtac
     4861 ctgctgatcg tgggcgacga ggaggcgggg gaggggaccg tcaacgtgcg ccggcggggc
     4921 gagaagcagc aggaggcggt cgcggtcgag gagttcgcgc ggcgggtcgg tgaggagatt
     4981 gcagcgcgcg attcatatag acagtagtgg cgacggcgcg gtataatctg gcttgtcgtt
     5041 tggggtccgc gcctgtccgg cgcctaatca tggcagtcca gtgagggcta gtggtcccga
     5101 aaaataggtg ttgagaggag tggtgcacag tagcaactga agaagagccg aggatcaacg
     5161 gacagatccg ggcacgctcg gtacggttga tctccgcgga tggcgagcag atgggtatca
     5221 agcacatccg cgaggctatg gattacgccg agaggctcga cctcgacgtg gtggaggtcg
     5281 caccgaacgc caacccgccc gtggtccgca tcatggacta cggcaagttc aagtataaga
     5341 aggaacagga ccgcaaggcc gcccgaaaga agcaggtcaa cgtcaacgtc cgggagataa
     5401 agctccggcc gaagatcggc gaccacgact tcgagacgaa gaagggccac gtcgtgcgct
     5461 tcctgaaggg cggtgacaag gtgaaggtca cgatcatgtt ccgcggacgc gaggtgcagc
     5521 accccgacct gggtgagcgg ctactccggc gactcgaaga ggacgttgcg gatctgggcc
     5581 gcgtcgagag ccagccgaac ctcgacgggc gtaacatggt aatggtcctg gccccgaaga
     5641 aggagcagaa agaagcctct tccgaggagc cgcaggagag cgcctcccga cgttaatccg
     5701 acactaacgc aacagtttgc tacaataccg cccggcggat cgtcaagcga tgcgccgaaa
     5761 gaggcgaacc ggcttcgact cccgtgccgc gagccctgtg agttgtagtg tagttagaga
     5821 cgaaacgtgg aggaaaacca gtgccgaaga tgaagaccca ccagggcgcc gccgggcgct
     5881 tcgaggttgg gaaaaagggc aagatcaagc gccgccgcag cgggcacaac cacatcctgg
     5941 agaaaaagag ccggaagcgg aagcgtcgcc tgaacacgga gacgtttatc cactcgtcgg
     6001 atgagaagcg gatcaagcgt ctgctggggg tgaggtagca tggcacggac cgcgcgcagt
     6061 ctccacgccc gcaagaagcg ccgcaaggta ctcgagcagg cgaagggtta ccgggggacc
     6121 aagcacagct cgtacaagcg ggccaaggag caggtctgga agagcggtgt ctacgcctac
     6181 cagggccgca agcagcgcaa gcgggatttc cgggcgctct ggatccagcg gatcaacgcc
     6241 ggtgttcggg agcacggact ctcctactcg cagttcatcc acggcctcaa gctggcggag
     6301 atagacctgg accgcaagat cctggccgac ctcgccgcca ccgagccgga agtcttcgcc
     6361 gctgtcgtaa cccaggcgaa gaacgccctg gaaggcaacc ctggcgagag ccgcgttaag
     6421 gtggagcgca gggccgagcc cgagccccgt ccgcaccctt ccaggcgcaa gtcccgcaag
     6481 accgagaagc ccgcgcacga ggaggctccc gagtcggctg cgaccgagga ggccgcgcag
     6541 gccgaggcgc cacaagccgt cgccgacgag ccggtcgcgg agcaggcgtc cgctgaagcc
     6601 gacgaagatg tcgaagccac cgaggccgcc gagcgcaagg ccgaggagct tgatgtggat
     6661 ctagaatccg tcgagggcac gggtgccgag ggacgagttc tcgtagagga tgtcgagcag
     6721 accgccgacg agcagggcaa ggtggtcgcc acagacaccg ccgaggagag ggccagagaa
     6781 ctcggtgtgg acctcgaatc cgtcgaaggg accggcgcga acggccgcat cactgtggaa
     6841 gacgtggaga aagccgcaaa caagtagccg gagcggatgt ccgtagcgtc ctacttgtga
     6901 tctctgctaa gcgggccgcg aggctcaacc agaagaagcg ccgcgagacc gaaaagcttt
     6961 tcctcgctga aggcgcgtcg ttcctggagg agtcccccga gccgcccgtg gcgctcttcg
     7021 aaacgaccga ggaagtgcgg cgcatatcca gctttacgac cccggccggc ccggtcgggg
     7081 tcttcccatt tgtggacgtg gaaccagagc ggctcctgca aagttgcgag agggtcatcc
     7141 tgctgcacgg cgtccaggac cccggtaacg tcggcacgat catccgcgcc gcgcacgcct
     7201 tcggggcggg cgtcgccctc tcgataggct gcgcggacct ctacaacccc aagaccgtgc
     7261 gggccacgat gggctccata ttccacgcgc cggtagcgag agacctcgac tcgctagatt
     7321 tcatccgtat ggccgacgca gcggagttcg ttcgggtcgc cgctgtggtg gaaacgggag
     7381 gagccgcgct cgaccgtctg gcaggcaagg tgcttatagt cgtcggagcg gagggtaagg
     7441 ggctgccgga ggagatcatt tcagcctgcg atagaggcgt gactatacct tcccgcgcag
     7501 cctcgctcaa cgccgcgatg gcagcttcca tactgctcta cgaggcgtat aggcgtgtgc
     7561 taccatagcg cccactatga cgaccgccag ccggatagag gagcttaaga gcgaggccct
     7621 ggcggcggta tccgccgccg gttctaccgc cgatctcgag agcgttcggg tacggtatct
     7681 cggccgctcg gccgagctaa cggagatcaa gaagagtatc ggcactctgg ccccggatca
     7741 gcgcaaggag gtaggtgggt ccgctaacag ggcttcccgt gcggtcgagg cgtccatctc
     7801 ggagcggtta gaggagttat ctgtaaggga acgcgaagag cgtctgcggg ccgaggccgt
     7861 ggacgttact cttcccggcg tgccggttcc caagggacac ctgcatccct cgcagcggat
     7921 cattgacgag gtcgtggact tcttcgtcgg gctcggttac agggtcgcgg aggggccgga
     7981 ggtggagacc gactactaca acttcacggc gctgaacata ccgcccggac acccggcgcg
     8041 cgggatgcaa gacacgttct ttctggacga cgggcttgtc ttacggacgc acacctcgcc
     8101 ggtccaggtg cggacgatgc tctcgcagga gccgccggtc tacgtggtgt gtccggggcg
     8161 cacttaccgg cgcgactcgg acccgacgca caccccgatg ttccaccaaa tcgaaggtct
     8221 cgccgtggac cggggactga cgctcgccca tctcaagggc acgctcgcgg cgatggcgcg
     8281 gcacgttttc ggagatagag tgcggctgcg gctgcggccg agctacttcc agttcacgga
     8341 gccgagtgtc gagatggacg tgagctgctt cgtctgtgat ggaacggacc ccgactgcaa
     8401 ggtctgcaag ggcgccggct ggctggagat gggcggggcg gggatggtag acccggcggt
     8461 gctcgaagag gtcggctacg actccgagga gtacacgggg ttcgccttcg ggttcggtcc
     8521 cgaccgcatg gcgatggtca agtacgacat cccggacctg cggctctttt tcgagggaga
     8581 cctccgcttc ctccggcagt tttagtttct agaaggagac agacctttgc gcgttccgtt
     8641 gagctggttg cacgagtatg tggacttcga cctctccgta gaggagctgg tagacctctt
     8701 tagtcttcgc agccaggaga tcgacggcgt ctaccggctc ggcgtgctcg acggtgaagt
     8761 cgtagtgggg gaggtgcagg agttcggacc ccaccccaac gccgaccggc tctcggtcgc
     8821 taaggtagac ctcgacgggc aggtcgtcca gatagtagcc ggcgcgccca acccgtaccc
     8881 cggcgcccgc gtcccggtcg tccttccagg gagcgttatg gccgacggca cgaagctcaa
     8941 gaaagcgaag ctccgtggcc tggagtccta cggcatgatg atgagcgagc gagagctcgg
     9001 gatctcgggc gaccacggtg gcatcctgct gctcgatgag tcctatgagg tcggccgtcc
     9061 cgtctcggac tatttccctg ccggagaggt cgtcatagac atagacgtta ctcccaaccg
     9121 accggacctg tggggcatga tcggcgtcgc ccgcgaactg gccgccatcc tgcagacggg
     9181 tttccgcatc cccgaagtct cgttcgagac gacgaatggc aagccaaccg gcgagtacac
     9241 gctgcgggtc gaggcggagg acctgtgccc gcgctacgac ctgcgccgta tctctgaact
     9301 aactcccgga cggcaggccc cgctggagat gcgccgtcgc atctacgccg ccggcatgcg
     9361 gcccatcgga gccgtcgtgg acgccaccaa ctacgtcatg ctggagacgg gacagccgat
     9421 ccacgccttc gacgccgaga aggtccgcga gggcatcgta gtccgtagag gcagggaagg
     9481 cgaaaagatc acgctgctcg acggctcgac ccggactctg gatgaggaga tgctggtgat
     9541 cgccgatgag gagcgcggcc tggtcatcgc cggagtgatg ggcgctgagg atgcggaagt
     9601 gagcgagcaa acgaccgaca cactgatcga ggtcgccaac ttcgacggga cgaacatcct
     9661 caggacttcg cagcgcctcg gtctacgcac cgacgcctct ggccggttcg agcgcggtct
     9721 ggacccgaac atggtggatt atgcgatgga tcgggtcact gctctcctca cagcatatcc
     9781 cggcggcgag gcggcagaag acaccctgag ccactacccg gagccggtga ggaaatggca
     9841 agtcccgctg cgccccgagc gggcgaggct gctacttggc atgccggtgg acgaggacga
     9901 tgcggcgcgg cggctggagt cgctcggatg cgaggtccgg ccggaggacg gcaggctcct
     9961 cgtaaccgcg cccaccttcc gccgggacct tcggcgcgag gcggacctca tcgaggaggt
    10021 cgggcgcttg atcggccttg accgggtccc ggaggagatc cctggcgtac ccacccccgg
    10081 tggtctcacc ccggagcagc gcaagttgcg cttgctccga caccttctcg cggacctcgg
    10141 tcttgcggag gccatcacct acccattcgg ccccgaccgg tgggaccggg atctcgctcc
    10201 cggcgccgaa gcctccgagg actgcatccg cgtgaagaat cctctcagcg ccgaggccgc
    10261 gaacctgcgc tctctcctgc tgccgggtct cctggatgcc gctgcccgca accggacgtt
    10321 cggtgcgcgg ggcggcgcgc tcttcgaggt cgggcatgtc ttcgaaccca gcccggtttc
    10381 cgaagagcaa cgcgaggttg cgctgcgctt ccggctcacg ggtgcgaaac ctgatgccga
    10441 cgccacagcg gacggtctta tgggcgtgag cgaggacttg agactagggg tagtccttgc
    10501 cgggaccacg cgccctgtcg gctggaacgc tcctgagtct cgagcgaact actttcaggc
    10561 aaaggggata gtggagcgga tcttgccgga cgccgtattc gaggcgtccg cgaagccatt
    10621 cctgcatccc ggacgcgcgg ctgtagtccg ggtgggtagc atagaggtcg ggtgggtggg
    10681 cgagttgcat ccggctacca tcgagggctt cgacctggaa ggatggccgg tcgcggcgtt
    10741 cgagctggac gtggcgtact gcgatccgga tccagagccc agattcacgc ccttcgtaaa
    10801 cgtgcccgcc gtcagccggg acctcgccgt gatcctgcgc gcggacaccc ttgtaggaga
    10861 catactcggt gcggtaaaga ggctcgggag cgagatactc gtcgaggagc gcgtcttcga
    10921 cgtgtacgag ggaccgcagg tcccggaagg aaccaagagc gtcgcgctga acttcacgtt
    10981 ccagggccgc gagacgctca ccgacgaacg ggtcgaagac gagatgcgcc ggatctcgga
    11041 gcgccttacg caggagttcg acgcccgcct gagagactcc tagcccgaac cctctcgcca
    11101 ctcgcttgcg aggatagaca taaggatggc gtcgtgacgc ttcccttcac gttcgagggc
    11161 cgcgcgtagc cgtccctcct cccggaaccc gagggctgtg tacgctctga tcgccgcttc
    11221 attgaagtcg aagacgctca ggccgacgcg cccgaggccg cgctcctcga acccgtagcg
    11281 caggatcgcg cgtatcgcat ccgtgccgta gccgcgtcct cgggcctcag gtgggcctat
    11341 gatgatcgag aggtccgccg tgccgttcgc ctcgctgata ttcatgaggc ttactatccc
    11401 gaccggaccg cgtccttctt tcgggtggat ggcgaaggag tcctccgtcg tggagagttc
    11461 ccggtcctcg aagagcctgc gcacggcgct tccggtgagc ggctcgccgg cccaactggt
    11521 gagccgccag atctccgggt cgccgtacca ccggccgtag agctcgtagt ccatgctctc
    11581 gtggcggcga agctcgaccc gctcaccgac cagcttcctg gatctcccgg acagacgcac
    11641 ggcctcaaat ataccgggtt ggaagagacg gcgctatgct agactgccct gctatgaagg
    11701 tggttccgga tcgcatagtc tcgctgggct tcgggaagta cgtgcgcgcg gacaagatag
    11761 ccgccctggt gcccatcgag gaggaccgcg gcagtggacg ccgcacgctc gtctacgtcg
    11821 agggtatgaa ggagccgctc gtagccggcc gcacggagtc aactatactg cgagacatgg
    11881 tgggggccga gagcggggcc atggaactcc tacgcgagct tcagatgcag atcgggcagg
    11941 tccggccgct gctcaagggc tccatccgct ccgaggccgg actcgacctg gaccagctct
    12001 cccggcgcat caccaacctg tccggcgagc aggaatcccc gcggatattc tagggaccgg
    12061 cttctatgac agagactccc gggaggtcgc cgtagacctc ctcggctgcg tgttgagcca
    12121 tgagtcctcc gaaggtctca cctccggggt catcatcgag accgaagcct accggccgga
    12181 cgatcccgcc tgccacgcct acaaggggcc tacgatgcgg aaccgcacga tcttcggcgc
    12241 gccaggtctc gcttacgtct acctgtcgta cggggtacac cacctgatca acgccgtctg
    12301 cgaggaggag gggacgggca gcgcggtcct gatccgtgcc ctacgcccac ttgaaggaga
    12361 ggacctgatg gccgaaagac gcggccgaaa cgaccatctg tgcgaagggc cgggccgtct
    12421 tacccaggca ctcggcattg atctctcgaa agacggtcac gacctgaccg atggtgggct
    12481 caccatccgc cctggcccgc ctcccgaagc cgaaatcgtg gcgacgaccc gcatagggat
    12541 atcgcggggc acagaccttc cgtggcgcta cctagtgctc ggtgacccca atgtttcgct
    12601 gccgccgcgc ggcatctcgc ggcgcggaat ttcgcgttcg gacttattag attagggctg
    12661 ggtggtcgag gtcgtgccgg tatctgctgg tgcctcagta actgttcccg tggacgtccc
    12721 cgtctgtatc tcggtcgtgc cgttggcggc gttgaccgtg ttggcggcgg tggtcgagtc
    12781 aacgcccttg aactgttcga cgggcttgcc gtccatgacc tccagcatgt agtcgtgcca
    12841 gatctccgtc ggagatccca cctgtccgta catcgagccc tctagcgggt cgagcagttc
    12901 ctggagcgtc gcgccgccct ccccgtagcc catccagaca cccgtcgcca gttgtggcgt
    12961 gaagccctcg aaccaggcgt cgaagaagtt ctcgctcgtg ccggtctttc cggccaccgg
    13021 cctgtccggg agggctgccc tgcccgctat gccgttcgta atatcgccca tcatgatctc
    13081 ggtcgccttg gcggcgacct tgggatctat gacctgcttt cccttgggct tgccagccgt
    13141 gaagaggact ttctcatcct tctgtccctc ttgttcgacg acctttgtga tcgccgtggg
    13201 ctctatccgc ttgccgccgt tggcgatggt cgcgtaggcc gtcgccatgt cgagcggcga
    13261 gacttctttc gcgccgagga caatagacgg atgaggatgc ttgccgaaat ccgccgtgat
    13321 gccgagcttg ttcgcgacgc gcgcgatgct ctccggtccg ttcttaagcc ccttgccgtc
    13381 cgtgttcatg gcgaggtcgg taaagaccgt gttgtccgac tgccaaagcg cctcctcgat
    13441 gcttatcgga ccgcgttcct tctttccaaa gttctccact ttccaacgtt ccgtgccgcc
    13501 ggaaccattt ggcacctcat agactttctt ctccgagacg aattgagtca tcgggtctat
    13561 tccctgttcc agagccgcta ttagcgcgaa cggtttgaac gagctgccgg gctgccgctg
    13621 ggcctgggta acgaggttga agtgcgatga ctggtcccgg ttcccgacca tggcctttat
    13681 ctggccggtc ttgggctcta tggagacgag agcggcgtct ggatcgtccg gggagggcag
    13741 gtagccgttc ggaccgtata tggtgtcctg ggccgcgacc tgggccttga gatcgagggt
    13801 cgtgtacacg ctcataccgc cgttcaggac ggtcgtggag ccgtatttgt cgatcaattc
    13861 ctgctggacc agctcggtga agttcctggt gagttgtggt cccgtaagcc ccgactcgac
    13921 catcggcgcg acgggccact cttttggaag ctgctcgtcc agcgccttat cccggtcctg
    13981 ctgggtcaga tagcccgcgt cgaacatctt cttcagtacc aggtcgcgct gttcggtggc
    14041 tgcgtcgcgg tccgagccga gggtagaagg cgaccagagg attccgatga gcgcggcgga
    14101 ttcgccagtg gtcaggtcgt ctacggactt gttgaagtag gtctctgccg ccgcctccac
    14161 accgtaggcg ttagacccga agtaaacagt gttcaagtaa tcggcgagga tctggtcctt
    14221 gttcttgtag gtgctccgct cgatctcgac ggcaatgagt gcttctttga tcttccgcgt
    14281 ccaggtctgg ttctggttga ggtaggcgtt ctttacgtac tgctgggtta tggtgctggc
    14341 gccctcgacc ttcttgcccg cccggaagtc cacccacagc gcgcgcatga ttccccagag
    14401 atccacccct ccatgttccc tgaaccgctc gtcctccttg gcgacgagcg cgttcagcag
    14461 gttgggcggc atctcatcca gcgaggcggt cttccggttt tcaccctgga agatagttcc
    14521 gatcacccgc cgcgggccgt catcgccccc gagcggcgcg gagtatatgt acgttggatg
    14581 gctctccacg ctctgcggtt tgtccaactg accgacgctc ttcacgagcc ccaggtacgc
    14641 tcctatacag aacgccagtc ccgcgacgaa gaggcacaga aagaagattc ccaggaccct
    14701 gaacactttg gttcggaagt tgccaccacc cgagcggcct ttcttggatt tcgacggtac
    14761 gcggagtggc cgccccgaaa ctgcgcgcct ctgtcctccg accctgtttc tgggataggt
    14821 acgagccatc ggttgggtat actacaacac cggagccccc gttacattgt cgttgcggat
    14881 aagttacatt tgtttaatga ccgacatact taatttcagc tccatttttc agaatgctgt
    14941 cgacaacgtc ccggccgatg aactcggacg ccggcttcgg agtggcgagg ccttacgggt
    15001 caaactcggg ctggatccaa ccgctcctga tatccatctg gggcacgccg tggtcctcgg
    15061 caagctgaga gagttccaga agctcgggca cacggcggta ctggtagtcg gcgactacac
    15121 ggcgagagtt ggagacccgt ccgggcgatc caagacccgg cccgtactca cgccggagga
    15181 gatcgaggcg aacattcgca cctacctgga gcaggcgtac ttgatcctgg atccggacca
    15241 gacggaggtg cgccacaact cagagtggct cgcgcctctc acgctcgccg acatcatcca
    15301 cctcacccgc gccaccaccg tcgctcgcat cctggagcgt gacgacttca gcaagcggta
    15361 cgcggcgaac gaccagatct ccctgaccga actcctctac ccgctcatgc aggcctacga
    15421 ctcggtcgcc atagacgccg atgtcgagct aggaggcacg gaccagctct acaacctcct
    15481 gatgggacgt cagatcatgg agtattacgg taagcgtccc cagtgcgtgc tgaccacccc
    15541 gttgctcgtc ggcaccgaag ggaagatgaa gatgagcaag tccctcggca actatattgg
    15601 cgtctccgag cctccgaacg atatgttcgg aaaggccatg agtatccccg acggcctcat
    15661 gcccgagtat tattctctgg tgctcggccg caccccgccc gatgcggagc ccgtcaggca
    15721 gaagcgggaa ctcgcccgtt ccctcgtccg taccttccac gggcaagacg cagttgaggg
    15781 cgcggagcag accttcgata cggttgtacg ccggggcgtg cccgaagacg tacccgaagt
    15841 cgcgttgccg gaaggagatc acgcctggat agtggacctg gtcgtgcggg ccggctttgc
    15901 gaagaccaac ggagaggctc gccggttcat aaaaggtggg gcggttcgca tcggcggaga
    15961 ggtactcacc gacgagcggg ccgcgctgcc gaccaaagct ctctccggga agatcctcca
    16021 ggtagggaag cgccgctatg cgcggtttat cgcctccggc tagacgcttt tcaagaaaat
    16081 tcagcaaacc gcttgacgtg ggaggaagtg ccgctacaat acctctcgcc gacat
//
LOCUS       DASSMB010000222         4154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_695606, whole genome shotgun sequence.
ACCESSION   DASSMB010000222 DASSMB010000000
VERSION     DASSMB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695606"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1145)
                     /locus_tag="VFJ72_13235"
     CDS             complement(162..1145)
                     /locus_tag="VFJ72_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7480465.1"
                     /translation="MKIPGIRLVKKAEQTGNEVDVPLTRGRLGLVDLLKRTFKEAGED
                     HLAAFAGNLTYKGLFALFPFFVFLLSLLGLFGSDTLLPKLLDSASGVLPQGATTFLRD
                     QLLTIADNKAKDTFTIGAIISILFALWGVSGAFRSVMEAMNVMYEVDEARPFWKVYGI
                     SIFLSLIVAVLLISALVLVVFGPQLAALVAGIVGFGQVFQILWIVVQWPVLVCVVLLA
                     FALIYYFAPDVEQRFRYVSPGSIVAVILWLLFSLAFSLYVNNFGGQSYSASYGALAGV
                     AILMLYIYYTTYILLMGAEMNQVIEEHAPEGKNEGEKTFNAQERAEEKGQT"
     gene            complement(1269..2447)
                     /locus_tag="VFJ72_13240"
     CDS             complement(1269..2447)
                     /locus_tag="VFJ72_13240"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7480466.1"
                     /translation="MRDVFVIDGARTAFGSFGGGLKDVTSQQMGVAAAKGAMERSGVD
                     PSKVDNVVMGNVIQTHEGAPYLARHVALDAGVPIETPALTLNRLCGSGLQAVVSATQS
                     IKLGESEIGLAGGSESMSQAPYVSRKARWGVRMGPDTLVDTLSEALADNRAGCGMGVT
                     AENLAEKYGITREDQDAFAARSHQEATRATQSGRLEDEMVTVTVQTRKGPVEINKDEH
                     IREGATQEGLSKLRPAFKKDGTVTPGNSSGINDGAAMLVVVSEDAVNEEGLTPMGRIV
                     SWAVAGVEPTIMGIGPVPASRLALKRANLTIDDVDLIELNEAFAAQYLACEEELGLDR
                     EKVNVNGGAVAFGHPVGASGARILLSLLYELKRREKKYGLATLCIGGGQGIAMVVESV
                     "
     gene            complement(2485..3534)
                     /locus_tag="VFJ72_13245"
     CDS             complement(2485..3534)
                     /locus_tag="VFJ72_13245"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7480467.1"
                     /translation="MAPTSMTAARFYEVNEPLRLEEIPVPEIQEDEVLLQMKAAGLCG
                     SDVHIVFEGVTPTAFKPITLGHEPAGVVAATGARVEGWEEGDRACVVPGLFCGSCQNC
                     VMGFPNICLNRNILGIQREGALAEYLVVPAKNLVRLPESVPFTVGAIITDAVATPFYA
                     MTERVALRAGETVAIFGAGGLGLHAVQISRLLGAAKVIVVDPRDDQLERARKMGADIT
                     INPQETQPVEAILEATGGLGVDVAAEFVGLQQTITQCVESVVMGGRVVVVGLGPDPIS
                     IVPPTVFVRKQIALLGSYGFTKRGIEQLVELSATGKLKLEESITHTFPLEEVNTALKY
                     LHEKIENPIRVAVTF"
     gene            complement(3553..>4154)
                     /locus_tag="VFJ72_13250"
     CDS             complement(3553..>4154)
                     /locus_tag="VFJ72_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7480468.1"
                     /translation="VNPPDRPKLQCLGVPHFDVDARVVDPATLEELGPNEEGEIVVSG
                     PQVMRGYWKRPEADEEAFFERDGKRFFRTGDIAVYDDEGYFFMVDRLKRMINASGYKV
                     WPSEVESILYKHPAIQEACVIGIPDEQRGETAKAVVVLREGEDVSAEEIIEWSKGEMA
                     AYKYPRSVEFTDALPKSGSGKILWRELQEKEQQKMAEAK"
BASE COUNT          674 a         1460 c         1274 g          746 t
ORIGIN      
        1 cctacggcgg catcttcatc gtcatggcgc tgggctgggg cgtcgtggtg gacggcttcc
       61 ggcccgaccg ctacgacctg ctcggagccc tgatctgcgt ggcgggcgta ctggtcatgg
      121 tagccccgca acgcggatag agcgcagggt ccagcgccat cctacgtctg gcccttctct
      181 tccgctctct cctgcgcatt gaaagtcttc tcgccctcgt tcttgccctc gggggcgtgc
      241 tcctcgatca cctggttcat ctccgcgccc atcaggagta tgtaggtggt gtagtagatg
      301 tagagcatca ggatggcgac gcccgcgaga gcgccgtagc tcgcgctata ggactggccg
      361 ccgaagttat tcacgtagag cgagaaggcg agggagaaga ggagccagag tatcacggcg
      421 acgatggagc cggggctcac gtagcggaag cgctgctcca cgtccggggc gaagtagtag
      481 atcagggcga aggcgagcag gacaacgcac accagcaccg gccactggac gacgatccag
      541 agtatctgga agacctgtcc gaagcccact atcccggcga cgagggccgc tagctgtggc
      601 ccgaacacga cgagcaccag cgcggagatc aagagcacgg ccacgatcag ggagaggaag
      661 atggagatgc cgtatacctt ccagaagggc cgcgcctcat cgacctcgta catcacgttc
      721 atcgcctcca tgaccgagcg gaacgccccc gagactcccc acaacgcgaa taggatggag
      781 ataatcgccc cgatggtgaa ggtatctttc gccttgttgt cggcgatggt gaggagctgg
      841 tcccggagaa atgtcgtcgc gccctgcggc agcacgccgg aggcggagtc gagcagcttc
      901 ggcagcaggg tatcggaccc gaagagcccg agcagcgaga gcaggaacac gaagaacggg
      961 aagagggcga agagcccctt gtaggtgagg ttgcccgcga aggccgcgag gtggtcctcc
     1021 cccgcctcct tgaacgtcct cttgagcagg tccacgagcc cgagacgtcc tcgcgtgagc
     1081 ggcacgtcca cttcgttgcc ggtctgctcc gccttcttga ccaacctgat gccggggatc
     1141 ttcaacgcgc cctccctgat agcgccgcct gtacttcccc caatctatac ccaatccgcc
     1201 ggaagagcca gaggactcga atcttcggtc cccggctctc ccggtgtcga gctagtctct
     1261 tgcgagattc agaccgactc gaccaccatc gctatgccct gcccgccgcc gatgcagagg
     1321 gtggcgagac cgtacttctt ctcccgccgc ttcagctcgt agaggagcga gagcaggatg
     1381 cgcgccccgc tcgccccgac ggggtggccg aaggcgaccg cgccgccgtt tacgttgacc
     1441 ttctcccggt cgagaccgag ctcctcctcg caggcgaggt actgggcggc gaaggcttcg
     1501 ttcagctcga tcaggtcaac gtcgtctatc gtgaggttcg cccgcttgag cgccagacgc
     1561 gaggccggga ccggacctat ccccatgatg gtcggctcca cgcccgctac cgcccagctc
     1621 acgatacggc ccatcggcgt caagccctcc tcgtttaccg catcctccga gaccacgacg
     1681 agcatggccg cgccgtcgtt gatgccgctg gagttcccgg gcgtgaccgt gccgtccttc
     1741 ttgaaggcgg gcctgagctt ggagagcccc tcctgcgtcg cgccctcgcg gatgtgctcg
     1801 tccttgttta tctcgaccgg acccttgcgg gtctgtacgg tgacagtcac catctcgtct
     1861 tccaggcgtc cgctctgggt tgcccgtgta gcctcttgat ggctccgggc ggcgaaggcg
     1921 tcctggtcct cgcgcgttat gccgtacttc tccgccaggt tctccgccgt cacgcccata
     1981 ccgcagccgg cgcggttgtc cgccagcgcc tcgctgaggg tgtccacgag cgtgtccggt
     2041 cccatacgca cgccccagcg ggccttgcgc gagacgtagg gggcctggct catgctctcc
     2101 gagccgcccg ccagcccgat ctcggactcc cccagcttga tggactgggt cgccgagacg
     2161 acggcctgga gtcccgagcc gcagagccgg ttcagggtga gggccggtgt ctctatcggg
     2221 acgccggcgt ccagggcgac gtggcgggcc aggtacgggg cgccctcgtg agtctggatc
     2281 acgttcccca tcaccacgtt gtccaccttc gaggggtcaa cgccgctgcg ctccatcgcg
     2341 cccttcgccg ccgcgacgcc catctgctgg ctcgtcacgt ccttcagacc gcctccgaac
     2401 gaaccgaacg ccgtccgggc tccgtcgata acgaatacgt ctctcatgcc gctcctctca
     2461 aatacgccgc tccgcgggca ctgctcagaa tgtcaccgcg acgcggattg ggttctctat
     2521 cttctcgtgc aggtacttca gggccgtgtt cacctcctcg agcgggaagg tgtgggtgat
     2581 cgactcttcg agcttcagct tgccggtggc ggagagctcg acgagctgct cgatgccgcg
     2641 cttcgtgaag ccgtaggagc cgagtaacgc aatctgtttg cgaacgaaca ccgtcggcgg
     2701 gacgatgctg atcgggtccg gcccgagtcc cacgaccacg accctgccgc ccatcaccac
     2761 cgactcgacg cactgcgtga tggtctgctg cagcccgacg aactcggcgg ccacgtccac
     2821 gccgagcccg cccgtcgcct ccaaaatggc ctcgacgggc tgcgtctcct gcgggttgat
     2881 ggtgatgtcg gcgcccattt tccgggcgcg ttcgagctgg tcgtcacgcg ggtccacgac
     2941 tatgaccttc gcggccccga gcaagcgtga gatctgtacg gcatgcaacc cgaggccgcc
     3001 cgcgccgaag atagccaccg tctcgcccgc gcgcagggcg acccgttcgg tcatcgcgta
     3061 gaacggcgtc gccaccgcgt ccgtgatgat cgcgccgacc gtaaacggca cgctctccgg
     3121 caaccgcaca aggttcttgg ccgggaccac caggtactcg gcgagagccc cttcccgctg
     3181 gatgccgagt atattgcggt tcaggcagat gttggggaac cccatcacgc agttctggca
     3241 cgagccgcag aagagacccg gcacgacgca ggcccggtct ccctcctccc agccctcgac
     3301 ccgcgcgccc gtcgcggcga ccacccccgc cggctcgtgg cccagcgtga tcggcttgaa
     3361 cgccgtcggc gtcacgccct cgaagacgat gtgaacgtcc gagccgcaga gcccggccgc
     3421 cttcatctgg agcaggacct cgtcctcctg tatctccggc acggggatct cctccagccg
     3481 cagcggctcg ttgacctcgt agaaacgggc cgccgtcatg ctcgtcggcg ccataagcac
     3541 tccttccggt agctacttcg cctccgccat cttttgctgc tccttctcct gaagctcgcg
     3601 ccacaggatc ttgccggacc cgctcttcgg cagagcatcg gtgaactcca cgctccgcgg
     3661 gtacttgtag gcggccatct cccccttcga ccactcgatg atctcctcgg ccgacacgtc
     3721 ctctccttcc cgcaacacga ccacggcctt cgccgtctct ccgcgctgct cgtccgggat
     3781 gccgatgacg cacgcctcct ggatcgccgg gtgtttgtag aggatggact cgacctcgga
     3841 aggccacacc ttgtagccgg aggcgttgat catgcgcttg agccggtcca ccatgaagaa
     3901 gtagccctcg tcgtcgtaga ccgcgatgtc gcccgtcctg aagaaccgct tgccgtcgcg
     3961 ctcgaagaac gcctcctcgt cggcctcggg gcgtttccag tagccgcgca tcacctgcgg
     4021 gccgctgacc acgatctcac cctcctcgtt cggacccaac tcctccagcg tcgccgggtc
     4081 cacgacccgc gcgtccacgt cgaaatgcgg cacgccgagg cactggagct tgggccggtc
     4141 gggcgggttg acgt
//
LOCUS       DASSMB010000223         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1455711, whole genome shotgun sequence.
ACCESSION   DASSMB010000223 DASSMB010000000
VERSION     DASSMB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455711"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..592)
                     /locus_tag="VFJ72_13255"
     CDS             complement(419..592)
                     /locus_tag="VFJ72_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480469.1"
                     /translation="MSIAIVSLILLVVVAIVAIPVLIRVFGEAARRNPNRPGGPGDDE
                     TGERDRTSGPGRE"
     gene            complement(649..1110)
                     /locus_tag="VFJ72_13260"
     CDS             complement(649..1110)
                     /locus_tag="VFJ72_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480470.1"
                     /translation="MMGRAVRGAMGGAAGTVALTVLRQTLKHMGLVHTSAPEQVVARL
                     EELRVVHEWSPAAQRALSVAAHFAYGVGTGAFFGMLRRDRGGKVEEAAVGSALGILSW
                     GAGWSTWLPLAGVHEPPWRQRTWKVLLPVLDHAAFGVVWGLLYRALAREPQ"
     gene            complement(1175..>2347)
                     /locus_tag="VFJ72_13265"
     CDS             complement(1175..>2347)
                     /locus_tag="VFJ72_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET7480471.1"
                     /translation="VLLVSTLGAYAFGRSQSPAGISPQDRQAVSLYAEALDKVREDYV
                     DQKAVDPKKQTYAAIEGMLDSLGDEGHTRFLTPKEVKENQAGLSGTYVGIGVQLEDRD
                     GRAVIAAPIEGSPAERAGLKTGDVVIAVDGKNVRDLALSDIVDRVKGPEGTTVKLTVE
                     RDSRERTFSMERAEIRAPVASWAKIPGTDIAQVRLSSFSDDSAKELGKSLDEARAAGA
                     RRFVLDLRSNPGGRLDQATEIAGLFLKPGKVIYIRKDASGKREEVKVDDEAEPIGAPM
                     TVLVNEGSASSAEILAGALRDNGRAKVIGTRTFGTGTVLSEFDLRDGSAILLGIAEWL
                     TPDGDFIRETGIEPDIKVKLGKDEEPVTPSEARDLSEQQILDRDPQLRRAVQELED"
BASE COUNT          408 a          821 c          687 g          431 t
ORIGIN      
        1 tgcgaggatg aacgtgcctg cgatgcgcat gcgcgggccg tcgctacgaa acgagagccg
       61 cctggaccgc tcgtactcga caagttccgt gcgcagggaa cctgtcccct cgtataactc
      121 ttgaaaagtg gcgccgatac cgcttgtcga tacgtgccac cctggggttc cacgccgcct
      181 cattacggat atccgcgaga aagtcgaaca cctcctcaac gggacgtttc acggcacact
      241 ctaaacatgc ccttcatacc gctattttct cctcggcgat caccgcgcaa cttccttcag
      301 ggatcttcaa atagagtgtc cgccatcaca tccatgaacg gccaagcgac gatccggacg
      361 tgatgagcag cccacgagcg agcccgggat tactacaaat ctttagctct gacgcagact
      421 attccctgcc gggaccggag gtgcggtcgc gttccccggt ctcgtcatcc cccggtccac
      481 cgggacggtt ggggttgcgg cgggcagcct cgccgaacac ccggatgagg accggtatgg
      541 ctactatagc cactaccacg agaaggatta gagatactat cgcgatactc aagggctccc
      601 ctttctcaag tcgccggctt tcgtaccggc gctcgtatac taccaatctt attggggctc
      661 gcgcgccagc gcccggtaga ggagccccca caccacacca aacgccgcat ggtccagcac
      721 cggcaacagt accttccacg tccgctgacg ccacggtggc tcgtggaccc ctgcgagcgg
      781 cagccaggtg gaccacccag ccccccagga gaggataccg agcgctgagc ctacggcggc
      841 ttcctcgacc tttccaccgc ggtcccggcg taacatcccg aagaacgccc ccgttcccac
      901 accgtacgcg aagtgcgccg cgacggagag ggcgcgctgg gccgccggcg accactcatg
      961 gaccaccctt agctcttcga ggcgggcgac gacctgctcc ggagccgagg tgtgcacgag
     1021 gcccatgtgt ttcagggtct ggcgcaacac cgtcagcgcg accgtgccag ccgccccacc
     1081 catagctcca cgaaccgccc tgcccatcaa ccgcatcgca tacccccaaa agaccctgat
     1141 gttacaaatc cagtgtaccc ttagatgcgg gtggctagtc ttccagctcc tgcacggcgc
     1201 ggcggagttg agggtcgcgg tccaggatct gctgttcgga gaggtcgcgg gcttcggagg
     1261 gcgtgacggg ttcctcgtct ttgccgagct tcacctttat gtcgggttcg atgccggtct
     1321 cccggataaa gtctccgtcc ggcgtcagcc actcggcgat gccgagcagg atggccgagc
     1381 cgtcgcgcag gtcgaactcg gagagcacgg tccccgtccc gaaggtcctg gtgccgatga
     1441 ccttggcgcg gccgttatcg cggagggcgc cggccaggat ctcggcgctg gaggccgacc
     1501 cctcgttgac gagcaccgtc atcggggcgc ctatcggctc ggcctcgtcg tccaccttca
     1561 cctcctcgcg cttgccggag gcgtccttgc ggatgtagat gaccttcccg ggtttcaaga
     1621 agagaccggc gatctcggtc gcctgatcca gcctgccgcc gggattgctc cgcaggtcga
     1681 ggacgaatcg gcgcgcgccc gcggcccggg cctcatcgag agacttacca agctccttcg
     1741 cgctgtcatc ggagaaagag gagagcctca cctgggcgat gtccgtgccc gggatcttcg
     1801 cccatgaggc gacgggagcc cggatctccg cccgctccat cgagaacgtg cgctctctgg
     1861 agtcgcgctc gaccgtcagc tttacggtcg tcccctccgg tccctttacg cggtccacga
     1921 tgtccgagag agcgaggtca cgcacgtttt ttccgtccac ggcgatcacc acgtcgcccg
     1981 tcttgagccc ggcccgctcc gcgggagagc cttctatcgg cgcagcgatt acagcccgcc
     2041 catcccggtc ttctagctgc accccgatgc cgacgtaggt cccggagagt cccgcctggt
     2101 tctcctttac ctccttcgga gtcagaaacc gcgtgtgtcc ctcgtcgccc agagagtcga
     2161 gcatcccctc tatcgcggcg taggtctgct tcttcgggtc taccgccttc tggtccacgt
     2221 agtcttcccg gactttatcc agcgcctcgg catacagcga caccgcctga cggtcctgcg
     2281 ggcttatgcc agccggactc tgggaacgcc cgaaagcata cgcgccgagg gtcgaaacga
     2341 gcaggac
//
LOCUS       DASSMB010000224         2676 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_62949, whole genome shotgun sequence.
ACCESSION   DASSMB010000224 DASSMB010000000
VERSION     DASSMB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2676
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62949"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..125
                     /locus_tag="VFJ72_13270"
     CDS             33..125
                     /locus_tag="VFJ72_13270"
                     /inference="COORDINATES: protein motif:HMM:NF023298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HET7480472.1"
                     /translation="MPGPMKGWTDSREMPPLAPRSFRELWKEGI"
     gene            125..802
                     /locus_tag="VFJ72_13275"
     CDS             125..802
                     /locus_tag="VFJ72_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HET7480473.1"
                     /translation="MSDHTEFLQSIRHRTRAGKYRPTVAPDAVWTRTGETPEVEPIED
                     PPARFIQELEALGGHGRRVGSMDEAREYVLALARDREAKLLIRWDVEELDRLGVDGPL
                     AEAGTEVVPWRDLEDFREVARRAEIGLSTAEWAIAETGSLVLESGVGKGRTVTLLPPT
                     YVAVVPVDRVLRTVPEAIEKYADAGPGALPANVCFHTGPSRSGDIEMSLVVGMHGPGD
                     VHVILVG"
     regulatory      884..1041
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            1115..>2676
                     /locus_tag="VFJ72_13280"
     CDS             1115..>2676
                     /locus_tag="VFJ72_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HET7480474.1"
                     /translation="MADSTVSAQRPVARLQTWLLEHRLVETKGPGAKEGQEEQHSWWQ
                     VVCLTGVDYFSTLGYIPGIAGLAAGALSPVATLLIVLLTLFGMLPMYRRVSEHSPHGQ
                     GSIAMLESLLSFWKGKLLVLCLLGFVATAWLVTITLSAADATAHIVENPLVPDFFQGQ
                     RVIITLVLLAVLGAVFLKGFREAIGIAVALVAAYLLFNLVVVAVGMYEILTVPKNLAN
                     WQDSLFSNYGSPLAMLGVSLLVFPQLALGLSGFETGVSMMPLVRGKPDDDPEKPAGRI
                     RNTRKMLTVAALIMSFYLITTSFVTAVLIPAQEFKANGSANGRALAYLAHEYLGGVFG
                     TIYDLSTISILWFAGASAMAGLLNIVPRYLPRYGMAPRWTRALRPLVLVYVTIACAIT
                     IFFSASVDAQAGAYATGVLAMMTSAAFAVMLTAWRGGSRLVAAAFGLVFGIFIYAIIA
                     NEVQRPDGIVISLFFIGTIIITSLVSRIQRSTELRQERIELDKKAQKFIEEASKGEIH
                     IVAHRRRIGDDP"
BASE COUNT          427 a          851 c          919 g          479 t
ORIGIN      
        1 ggcccgcggt gaaagacggt tcggtcaggc cgatgcccgg tccgatgaag ggctggacgg
       61 actcgcggga gatgccgccg ctcgccccca ggtccttccg cgagttgtgg aaggagggga
      121 tctagtgtcc gatcacacgg agttcctcca gtccatccgc caccgcaccc gcgccgggaa
      181 gtaccgtccg accgtcgcac ccgatgctgt ctggacccgc acgggggaga cacccgaggt
      241 agaacctatc gaggaccctc cggcccgctt tatccaggag ctagaagcac tcggcgggca
      301 cggtcggcgg gtcggcagca tggacgaggc gcgggagtac gtgctggcgc tggcgcggga
      361 tcgggaggcc aagctcctca tccggtggga cgtggaggag ctggaccggc tcggcgtgga
      421 cggaccgctc gcggaggccg ggacggaggt cgttccctgg cgggacctcg aagatttccg
      481 ggaggtcgcg aggagggcgg agatcgggct ctcgaccgcc gagtgggcta tagccgagac
      541 cgggagcctg gtgctggaga gcggagtggg aaagggccgc acggtgacac tcctgccgcc
      601 gacttacgtg gccgtcgtgc cggtggaccg ggtcctgcgg acggtgccgg aggctatcga
      661 gaagtatgcc gatgccgggc ccggcgctct tccggccaac gtgtgcttcc acacgggacc
      721 gagccggtcg ggagacatag agatgtcgct ggtcgtcggg atgcacgggc cgggcgacgt
      781 gcacgtgata ctcgtaggct gacgtttctc gcgccctctg gcgttcgcct ccggcgttga
      841 caagatctaa tgtcgggagt atatgtgcga gcgtagatat cgtagccgta aagcgccgag
      901 ctccgggtgg ctccggaggc gggggaccca catcggattg caggagtcca ggggtgaatc
      961 gccagtgaaa gctagcgtag ggcttgacct cttttggccc gaacccgaca gctaacctcg
     1021 caggcgcgga agaaggaggt aatcgggtgt cccaggaaaa attctcccgg atacgctttc
     1081 cttggggctg tcgcacggct tccggggagt tgtaatggct gattccacag tttcggcgca
     1141 gcgtccggtg gcccgtctcc agacctggct gctcgaacac cggctcgtgg agacgaaggg
     1201 gccgggggcg aaggaggggc aggaggagca gcactcctgg tggcaggtcg tgtgcctcac
     1261 gggtgttgac tacttctcga cgctcggcta tatacccggc atcgcggggc ttgcggccgg
     1321 ggcgctctcg cccgtcgcca ccctgctcat cgtgctgctg acgctcttcg ggatgctccc
     1381 catgtaccgg cgcgtctccg agcacagccc gcacgggcag ggctccatag cgatgctgga
     1441 gagcctgctc tctttctgga agggcaagct gctggtcttg tgcctcctgg ggttcgtggc
     1501 gaccgcgtgg ctcgtcacga tcacgctctc ggcggcggac gccacggccc acatcgtcga
     1561 gaacccgctg gtcccggact tcttccaggg gcagagggtc attatcacgc tcgtgctgct
     1621 cgcggtgctc ggcgcggtct tcctcaaggg gttcagggag gcaatcggca tcgcggtcgc
     1681 cctcgttgcc gcatacctgc tgttcaacct ggtcgtcgtg gcggtcggga tgtacgagat
     1741 cctgacggtc cccaagaacc tcgccaactg gcaggactct ctcttctcca actacggtag
     1801 cccgctggcg atgctcgggg tgtcgctcct ggtgttcccg cagctcgccc tggggctctc
     1861 ggggttcgag accggcgtga gcatgatgcc gctcgtgcgc ggcaagccgg atgacgaccc
     1921 ggagaagccg gcgggccgca tccgcaacac gcgcaagatg ctgaccgtcg ccgccctgat
     1981 catgagcttc tacctcatca cgaccagctt cgtcacggcc gtgctcatcc cggcccagga
     2041 gttcaaggcc aatgggagcg ccaacgggcg cgctctcgcc tatctggcgc acgagtatct
     2101 gggcggcgtc ttcggcacca tctacgacct cagcacgatc agtatcctct ggttcgccgg
     2161 ggcgtcggcg atggctgggc tgttgaacat agtcccccgc tacctgcccc gctacggaat
     2221 ggctccccgg tggacccggg cgctccggcc gctggtgctc gtctacgtca ccatcgcctg
     2281 cgcgattacg atcttcttct cggccagcgt ggacgcgcag gccggggcct acgccaccgg
     2341 cgtcctggcg atgatgacct cggccgcctt cgccgtgatg ctgaccgcct ggcgcggagg
     2401 ctcaaggctc gtggcggcgg ccttcgggct cgtcttcggc atcttcattt acgccatcat
     2461 cgcgaacgag gtgcagcggc ccgacggcat cgtcatctcc ctgttcttta tcggcacgat
     2521 catcatcacc tccctcgtct cgcggataca gcgctccacg gagttgcgcc aggagaggat
     2581 agagctggac aaaaaggccc agaagttcat cgaggaggcg agcaagggtg agatccacat
     2641 agtcgcccac cgacgcagga tcggcgacga cccgga
//
LOCUS       DASSMB010000225         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1320455, whole genome shotgun sequence.
ACCESSION   DASSMB010000225 DASSMB010000000
VERSION     DASSMB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1320455"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFJ72_13285"
     CDS             <1..519
                     /locus_tag="VFJ72_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7480475.1"
                     /translation="REYPTEEDRMRLVIELSRLNVEHGTGGPFGAAIFDLETNRLVAP
                     GINLVTASNLSSAHAEVVAIMVAQQVIGHFDLGASGQPPRELAASTEPCSMCFGSVPW
                     SGVRRLVCGARDEDARAIGFDEGPKMPDWKAALEERGISVARDVCRDEAASVLRDYAE
                     RGGEIYNARAGY"
     gene            577..1110
                     /gene="uraD"
                     /locus_tag="VFJ72_13290"
     CDS             577..1110
                     /gene="uraD"
                     /locus_tag="VFJ72_13290"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HET7480476.1"
                     /translation="MRQKATVDEVNRMTKQEFVARFGTLYEHSPWVADGAYSQHPFGG
                     ISELHEAFARTVRDAPRERQVELIRSHPDLAGKAAMAGELTDESTAEQSSAGLDRLSP
                     QEYETFTRINREYRDKFGMPMVVAVREHTRESIIAQAKARLSHSRDEEIETALGEIDK
                     IAWIRLQDRVAAETGAG"
     gene            1132..1998
                     /gene="pucL"
                     /locus_tag="VFJ72_13295"
     CDS             1132..1998
                     /gene="pucL"
                     /locus_tag="VFJ72_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HET7480477.1"
                     /translation="MTETARGQIVLGQNNYGKSEVRVFKVKRDAERHEIWDLDIRISL
                     EGDFEAAHVEGDNTGLLATDTMRNTAYALAKDHLTGSIERYGLALVDHFLEAGPSVMG
                     CRVEITQFIWERIEVDGQPHEHSFVRQRGERKAKVWGDESGERKVKAGIGDVYVLKTT
                     NSGWEGFLRERYTTLPDTNDRILATIVTAKWEYNTTDADFDRLWDGVMQTTLKTLTDH
                     YSPSVQNTLYRMGRAVLESFPEIARIWYSFPNVHHIPYDLARFGMENNGEIFHATQDP
                     YGQIEGWVERRA"
     gene            1995..2348
                     /gene="uraH"
                     /locus_tag="VFJ72_13300"
     CDS             1995..2348
                     /gene="uraH"
                     /locus_tag="VFJ72_13300"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HET7480478.1"
                     /translation="MSGFLTTHVLDTAHGHPAAGMPLELFRIQDGQRRSLKTVTTNED
                     GRTDEPLLTESEFETGVYEIVFDVGAYFAGEPGVPEPPFLGRVPLRFGVADPESHYHV
                     PLLASPWSYSTYRGS"
     gene            2513..>2724
                     /locus_tag="VFJ72_13305"
     CDS             2513..>2724
                     /locus_tag="VFJ72_13305"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565766.1"
                     /note="Plays a crucial role on both purine and pyrimidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HET7480479.1"
                     /translation="MSSYDVVIRGGVVVTGSGVSESDVGISDGVVAAVEPELEGSAGM
                     EIDASGLHVFPGLIDAHVHFNEPGRT"
BASE COUNT          543 a          839 c          913 g          429 t
ORIGIN      
        1 cgcgagtacc cgaccgaaga ggaccggatg cggctcgtga tcgagctgtc gcgcctgaac
       61 gtggagcacg ggaccggcgg acctttcggg gccgcgatct tcgaccttga gacgaaccgc
      121 ctggtcgccc ccggcattaa cctggtcacg gcctcgaacc tctcctccgc ccacgcggag
      181 gtggtggcga tcatggtcgc gcaacaggtg atcggacact tcgacctggg agcctcgggt
      241 cagccgcccc gcgagctggc cgcgagcacc gagccgtgct cgatgtgctt cgggtcggtc
      301 ccgtggtcgg gcgtccggcg gctggtgtgc ggcgcccgcg acgaggacgc ccgcgccatc
      361 ggctttgacg agggccccaa gatgccggac tggaaggccg ccctggaaga acgcggcatc
      421 tccgtcgcca gggacgtctg ccgtgacgaa gccgcttcgg tcctgagaga ctacgccgaa
      481 cgcggaggcg agatctacaa cgcccgtgcg ggctactagc cgccggtaac caggttcgag
      541 acccggactc ggatgagtct ggcgaacagg aggacgatgc gacagaaggc cacagtcgac
      601 gaggtaaacc gtatgacaaa acaggagttc gtggcaaggt tcgggacgct ctacgagcac
      661 tcgccgtggg tggccgatgg ggcttactct cagcatccct tcggcggaat ctcggagctg
      721 catgaggctt tcgcgcggac cgtccgtgat gccccgcggg agcgacaagt cgaactcatc
      781 cggtctcacc cggaccttgc agggaaggcg gcgatggcgg gggagctgac cgacgagtcc
      841 acggcggagc agtcgtcggc gggcctggac cggctttcac cgcaggagta cgagaccttc
      901 acgcggatca accgcgagta tcgcgacaag ttcggcatgc cgatggtcgt cgcggtccgg
      961 gagcacacca gggagtcaat aatcgcgcag gcgaaagccc ggctaagcca ctcgcgagac
     1021 gaagagatag agaccgcgct gggcgagatc gacaagatcg cctggatccg tctgcaagac
     1081 cgggtcgcgg cagaaacagg cgcgggatag cccgcggaga ggagaatgag catgacagag
     1141 acggcgagag gccagatagt tctgggccag aacaactacg ggaagtccga ggtccgggtc
     1201 ttcaaggtga agcgcgacgc ggagcggcac gagatctggg atctcgacat ccgcatctcg
     1261 ctggagggcg acttcgaggc cgcccacgtc gagggcgaca acaccggcct cctggcgacc
     1321 gacacgatgc ggaacacggc ctacgcgctg gcgaaggacc acctcaccgg cagcatcgag
     1381 cggtacgggc tggctctcgt ggatcacttc ctggaggccg gtccgagcgt gatgggctgc
     1441 cgggtcgaga tcacgcagtt catctgggaa cgcatcgagg tggacggcca gccccacgag
     1501 cactccttcg tccgccagcg cggcgagcgg aaggcgaagg tgtggggcga cgagagcggc
     1561 gagcggaagg tgaaggctgg gatcggggac gtatacgtgc tcaagacgac caactccggc
     1621 tgggaaggct tcctgagaga gcggtacacc accctcccgg acacgaacga ccgcatcctc
     1681 gccaccatag tcacggcgaa gtgggagtac aacacgacgg acgcggactt cgaccggctg
     1741 tgggacggcg tgatgcagac gacactcaag accctcacgg accactacag tccctcggtc
     1801 cagaacaccc tgtaccgcat gggcagggcg gtcctggagt cgttcccgga gatagcgagg
     1861 atctggtact ccttcccgaa cgtccaccac atcccctacg atttggctcg gttcgggatg
     1921 gagaacaacg gtgagatctt ccacgccacc caggacccct acggccagat cgaaggctgg
     1981 gtagagcggc gcgcatgagc ggcttcctca ccacccacgt cctcgacacc gcccacggcc
     2041 atcccgccgc cgggatgccg ctggagctct tccgcataca agacggacag cggcgatccc
     2101 tcaaaacggt caccacgaac gaggacggcc gcaccgacga gccgctgctc accgaatcgg
     2161 agttcgagac cggcgtctac gagatcgtct tcgacgtggg cgcctacttc gccggagagc
     2221 cgggcgttcc ggagccgcca ttcctgggcc gggtgccgct ccgcttcggc gtcgccgatc
     2281 ccgaatccca ctatcatgtc ccactgctcg cctcaccctg gtcctactct acctaccggg
     2341 gaagctgagg agatcctttt ctatagaggc attcctgagc agggtccaga agaccgctcc
     2401 gtctggaagc ccccgaaaaa agtttgtaaa agtgttgaca ttccgttttt gcatctgtat
     2461 gatccgcaat acggaatcaa attattgatg ggtggaagag aagtagaggg taatgagtag
     2521 ttacgacgta gtgatccgtg ggggcgtcgt ggtaaccggt tcgggggtgt cggagtctga
     2581 cgtggggatc tccgacggcg tggtggccgc ggtcgagccg gagttggaag gctccgccgg
     2641 tatggagatt gacgcttccg ggttgcatgt gttccctggc ctcatagacg cgcacgttca
     2701 cttcaacgag ccgggacgga ccca
//
LOCUS       DASSMB010000226         5461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1697267, whole genome shotgun sequence.
ACCESSION   DASSMB010000226 DASSMB010000000
VERSION     DASSMB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1697267"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..961
                     /locus_tag="VFJ72_13310"
     CDS             218..961
                     /locus_tag="VFJ72_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7480480.1"
                     /translation="MAKLDGKVAIVTGASSGIGEATAEALAAEGAAVVVAARRKDRLD
                     DLTKRIEGNGGKVLSVECDVTEEEQAHALIQAAEREFGSVDILVNNAGVMLLSRIERG
                     LSDEWRQMFDVNVLGLLYATDAAVEVMKRQGSGHIVNISSVAGRKVRETGGVYSGTKF
                     AVGAISEGLRMELLGDNIRVTVVEPGAVATELATHITDEEAQQGMSRFAGIEILQAED
                     IANAIAYAVSQPQRVSVNEILIRPSQQAN"
     gene            complement(971..1807)
                     /locus_tag="VFJ72_13315"
     CDS             complement(971..1807)
                     /locus_tag="VFJ72_13315"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HET7480481.1"
                     /translation="MAKDAAPQGEKRERLVFGGMARWGGLDLYGPNYMSEAWRKDGEI
                     HVRVEPSNLWRPTDPLALRLSRMPIIRSVFFWARLLVQVVGSIWTLVFFAASMAALWL
                     FVYVMDLGRGVGGGFLGGLFGFFADFPILPLLVLFFAAMKFTSIGRYHGAEHKAVAAY
                     EKYGEVTLDNAKRSSRIHPRCGTNILSYIILAALIDPLIDGWWYAIAQFIFISEAWFM
                     FGQTRPSIAVGNFLQRYFTTSEPNRSELEVAVESMKQLLEAEEGEAPSPGGKRVRVPA
                     RF"
     gene            1957..3132
                     /locus_tag="VFJ72_13320"
     CDS             1957..3132
                     /locus_tag="VFJ72_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7480482.1"
                     /translation="MQDRGQTGGYGRERTLYLGVALVLALLLGGYLVYRLAPIVLVLL
                     LTLLFSIIISGPVGYLERRGIIRSVATLIVLGAIGLALYLAGMVIASTVETQARELSE
                     RLPTLLREIRVLASRLQESFGIDLGLDELRPNLIFDRIKGFLSGDTVAVAADIGGSIA
                     NGLSLAFVAFIATIYLVLNPKPLVNGFVSFFPAGSRQRVREILREMYHAVQRWFLGQL
                     TSMTLIGVLSAIALSIIGIDFAVLIGLVSGLISFIPFVGPVISVIPPVLLALVSHPIN
                     AVWVVLAYAGIQLVESNLIQPVVMSRAVSLHPATIVFALLIMGTLFGFVGLLLAVPLV
                     AALHVLVRELWIERMDRHGTDPDPPPKEDTVGVDPPKQRARRARRMLRAARDLLRRS"
     gene            complement(3065..4465)
                     /gene="accC"
                     /locus_tag="VFJ72_13325"
     CDS             complement(3065..4465)
                     /gene="accC"
                     /locus_tag="VFJ72_13325"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7480483.1"
                     /translation="MIGKVLVANRGEIALRVVRACRELGIRSVAVYSTADADSLHCAL
                     ADEAICIGPPPAAGSYLNIPAIISAAELAGADAIHPGYGFLAENARFAEICERVGIKF
                     VGPASRTIARMGDKAAARHAAEQAGVPITPGSDGICESATEVRRVGDRLGYPLVIKAS
                     AGGGGKGMRVVHSASGVEDAFTAASREAGAAFGDGAVYVEKYLEELRHIEVQVLADSS
                     GTVVAFPERDCSIQRRYQKLLEESPAPGLPHEAREGMMDAATALVDALGYEGAGTVEF
                     VYSEGEFYFIEMNTRLQVEHPVTEMVSGVDLVAEQLKVAAGEPLTVPEDVGTPEGHAI
                     EFRINAEDPRRNFLPQSGPVEFYNPPGGPGVRVDSHLYAGYKVPPYYDSLLAKLIVYA
                     ADREAAIRRGARALDEFAILGLETTLPLHLAILEDEDFRRGGVTTSFLAGKELRSDEG
                     GPLRLAASGAPSVPSA"
     gene            complement(4475..4963)
                     /gene="accB"
                     /locus_tag="VFJ72_13330"
     CDS             complement(4475..4963)
                     /gene="accB"
                     /locus_tag="VFJ72_13330"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET7480484.1"
                     /translation="MHELDETQQDGFERLPIDEVVRLVDLLKESGVEEIRVRRGDVEI
                     AVKAKAEAPAPQSAPASGSTPSVEPAPAPDEIPSESDGLHAVRSPVVGTFYRAPTPDA
                     ESYVEVGDRVAAGDTLCIVEAMKMMNEIPADISGEVVEVLAENSAGVEYDQPLFYLRP
                     EE"
     gene            5255..>5461
                     /locus_tag="VFJ72_13335"
     CDS             5255..>5461
                     /locus_tag="VFJ72_13335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7480485.1"
                     /translation="MSIRVLDPRVAQQVAAGEVVDRPASVVKELVENALDAEASRIEV
                     ELGEGGTASILVRDDGSGMSAEDAR"
BASE COUNT          956 a         1765 c         1779 g          961 t
ORIGIN      
        1 ctcgtccggg cggagtacac cgcggcggcg atagcgttgg gcgccgtcgg aggcacaatc
       61 ctgctggctg tcttcttcgt cctctccgcc gtcctcggca ccttcaactc cagctactgg
      121 acgctcgcct acctgcggct cgccgccccg cgagcggaca cctccgttca gtagtattgg
      181 tcttttagaa tcgttcgacg gatagaaagg ttctcgcatg gcgaagctcg acggtaaggt
      241 agcaatcgta accggagcct ccagcggtat cggcgaggcg acggcggagg ctctggcagc
      301 cgagggagcg gcggtcgtcg tcgcggcgcg gcggaaggac cgcctcgacg acctcacgaa
      361 gcgtatagag gggaacggcg ggaaggtgct ctccgtggag tgcgacgtta cggaagaaga
      421 gcaggcgcac gccctgatcc aggcggccga gcgggagttc gggagcgtgg atatcctggt
      481 caacaacgcg ggcgtgatgc tcctctcccg gatagaacgc ggcctctccg atgagtggcg
      541 ccaaatgttc gacgtgaacg tcctcggcct cctctacgcg accgacgcgg cggttgaagt
      601 gatgaagcgc caggggagcg ggcacatcgt caacatctcc agcgtcgccg ggcgcaaggt
      661 gcgcgagacc ggcggagtct actccgggac caagttcgcc gtcggcgcga tctcggaggg
      721 cctgcggatg gagcttctgg gagacaacat ccgcgtcacc gtcgtggagc cgggcgcggt
      781 cgccaccgag ctcgccacgc acatcaccga tgaggaagcg cagcagggca tgagccgctt
      841 cgcgggcatc gagatactcc aggccgagga catcgccaac gccatagcct acgctgtcag
      901 ccagccgcag cgcgtgagcg tcaacgagat cctgatccgc ccatcccagc aggctaatta
      961 gtaagggcgg ctagaacctc gcagggaccc ggacgcgctt gccgcccggc gagggcgcct
     1021 cgccttcctc ggcttcgagg agttgcttca tggactctac cgcgacctcc agctcgctcc
     1081 ggttgggctc ggaggtcgtg aagtagcgct gaaggaagtt accgacggcg attgagggcc
     1141 gcgtctgacc gaacatgaac cacgcctcgg agataaagat gaactgcgcg atggcgtacc
     1201 accagccgtc tatcagggga tcaataaggg cggccaggat tatgtacgag aggatgttgg
     1261 tgccgcagcg gggatggatg cggctggacc gcttcgcgtt gtccagcgtt acctctccgt
     1321 acttctcgta ggcggcgacg gccttatgct ccgcgccgtg gtagcgcccg atggaggtaa
     1381 acttcatcgc ggcgaagaag aggaccagga gcggcaggat cgggaagtcg gcgaagaacc
     1441 cgaagagacc gccgaggaac ccgccgccga ctccccggcc tagatccatg acgtacacga
     1501 agagccagag ggcggccatc gaggctgcga agaagaccag cgtccagatg gagccgacca
     1561 cctgaaccag gagccgcgcc cagaagaaga cggatcggat tatgggcatg cgggagagac
     1621 gcagggcaag ggggtccgtc gggcgccaca ggttcgaggg ctcgacgcgg acgtggatct
     1681 cgccgtcctt ccgccaagcc tcgctcatgt agttgggacc gtagaggtcg agcccacccc
     1741 agcgggccat gcccccaaag acgaggcgct ctctcttctc cccctggggg gcggcatcct
     1801 ttgccaagct tctccctcaa atattcggaa ccagaggaca aaagatagca cgtcggacag
     1861 cgtaatcgag gtttcgcgaa ggttccgcgc gcggcgacgg accttgcaaa gggtagaatg
     1921 ggcggcagac gagcgcgctc tcgatgggag aaagaggtgc aggaccgagg tcagaccgga
     1981 gggtacgggc gggagcggac cctgtattta ggtgtcgcgc tggtcctggc gctgctgctc
     2041 ggtggctatc tggtgtaccg gctcgcgccg atagtccttg tcttgctcct gacactgcta
     2101 ttctcgatca tcatcagcgg tccggtcgga tacctggagc gccgcggcat catacgaagc
     2161 gtcgccaccc tgatagtgct cggcgctatc ggtctcgcac tctacctggc gggcatggtc
     2221 atcgcttcga cggtggagac gcaggcaagg gaactatcgg agcggctacc tactctgctg
     2281 agggagatcc gggtcctcgc aagccgcctt caggagtcat tcggtataga cctggggctc
     2341 gacgagcttc gtccgaacct cattttcgac cggataaagg gtttcctttc cggtgatacg
     2401 gtcgcggttg cggcggatat cggggggagc atcgccaacg ggctctcgct ggcgttcgtg
     2461 gcgttcatcg cgacgatcta cctggtactc aacccgaagc cgctggtcaa cggtttcgtc
     2521 tccttcttcc cggcgggctc gcggcagagg gtgcgcgaga tcctgcgcga gatgtaccac
     2581 gcggtgcagc ggtggttcct cggccagctt acctccatga cgctcatcgg ggtgctctcg
     2641 gccatcgcgc tttcgatcat cgggatagac ttcgcggtgt taatagggct cgtatccggc
     2701 ctgatctcgt tcatcccgtt cgttgggccg gtgatctcgg tgataccgcc ggtgttgctg
     2761 gcgcttgtct cgcatcccat aaacgccgtc tgggtcgtac tggcctacgc gggcatccag
     2821 ctagtagagt cgaacctgat acagccggta gtgatgagtc gggccgtctc gctgcacccg
     2881 gcgacgatcg tcttcgccct cctgatcatg ggcaccctat tcggcttcgt gggcctgctc
     2941 ctggcagtgc cgctggtcgc cgccctacac gtcctggtgc gggagctgtg gatagagcgc
     3001 atggaccggc acggcaccga cccggacccg cctccgaagg aggatacggt gggggtcgac
     3061 cctcctaagc agagggcacg gagggcgcgc cggatgctgc gagccgcaag ggacctcctt
     3121 cgtcgctcct gagctctttt ccggccagga agcttgtcgt taccccaccc cgccggaagt
     3181 cctcgtcttc cagaatcgcc aggtgcagcg gaagcgtggt ctccaggccc aggatggcga
     3241 actcgtccag cgcccgagcc ccgcgacgga tcgccgcctc ccggtccgcc gcgtaaacga
     3301 tgagcttcgc cagcagcgag tcatagtacg gcgggacctt gtacccggca tacaggtgcg
     3361 agtcaacccg tactccggga ccgcccggcg ggttgtaaaa ctccaccgga ccggactggg
     3421 gcaggaagtt gcgccgcggg tcctcggcgt tgatcctgaa ctctatcgcg tgtccctccg
     3481 gcgtcccgac gtcctccggc accgtcagag gctccccggc ggcgaccttc agttgttcgg
     3541 cgacgaggtc caccccgctc accatctccg taaccgggtg ctcgacctga agccgcgtgt
     3601 tcatctctat aaagtagaac tcgccctccg agtatacgaa ctcgaccgtc cccgccccct
     3661 cgtagcccag ggcgtccacg agcgcggtcg cggcgtccat catgccctcg cgggcttcgt
     3721 gcggcagacc cggcgcggga gactcctcca ggagcttctg gtagcggcgc tggatcgagc
     3781 agtccctctc cgggaaagcg accaccgtcc ccgaggagtc ggcgagtacc tggacctcta
     3841 tgtgacgcag ctcctccagg tacttctcga cgtacacggc gccgtctccg aaggccgcgc
     3901 cggcctcccg gctcgcggcg gtgaatgcgt cctcgactcc ggacgcggag tggaccacgc
     3961 gcatcccctt gccgccgccg cccgcgctgg cctttatgac gagcggatag ccgagcctgt
     4021 ctccgacccg cctgacctcc gtcgcgctct cgcagatccc gtccgagccg ggtgttattg
     4081 ggacacccgc ctgctccgcg gcatggcggg cggcggcctt gtcacccatg cgggctatcg
     4141 tgcgggaagc gggaccgacg aacttgattc cgacgcgctc gcagatctcc gcgaagcgcg
     4201 cgttctcggc gaggaagccg tagccggggt gtattgcgtc cgcgccggct agctcggcgg
     4261 cggagattat cgccgggatg ttcaggtagc tgccggcggc gggcggcggt ccgatgcaga
     4321 tcgcctcgtc agccagggcg cagtgcagcg agtcggcgtc ggcggtcgag tatacggcca
     4381 cgctgcggat gccaagctcc cggcacgccc gcaccacgcg cagcgcgatc tccccccggt
     4441 tggcgacgag gaccttaccg atcacgtagg gcctctactc ttcagggcgc aggtagaaga
     4501 gcggctgatc gtactcgacg ccggccgagt tctcggccag gacctccacg acctcgccgg
     4561 agatgtcggc cgggatctcg ttcatcatct tcatcgcctc gacgatgcac aaagtatccc
     4621 cggccgcgac cctgtccccc acttccacgt atgactccgc atccggcgtc ggagcccggt
     4681 agaacgtccc gacgaccggc gagcggaccg cgtgcagccc atccgactcc gaggggatct
     4741 cgtcgggcgc gggcgcgggt tcgacggacg gcgtagaccc ggatgcggga gcgctctgag
     4801 gagccggagc ctccgccttc gccttcaccg cgatctccac gtcgccccgg cggacccgga
     4861 tctcctcgac cccgctctct tttaacaggt ccacgagccg gaccacctcg tctatcggca
     4921 gccgctcgaa cccatcctgc tgtgtctcgt ccaattcgtg catagccgcc aattctactg
     4981 taagaatgtt tgaaacgctt tctgtcaacc gtggcaggac gtaaaagacg gcccctccgc
     5041 agattacttg gcggttatca gtggagaggg cctcgaacca aaggagactg attcctatgg
     5101 ctcgtagcca cattgtacgc gagagcgtac ccgaagttca cgtccgtaag cagcatcgcc
     5161 gaagcgtagc tgaagtatca ctggttggaa agccgtccgc cgcctgtaca atgctctagg
     5221 caatgatgga acgctctcgc ataccggagt ttgcatgagc atccgggtac tcgacccgcg
     5281 agtggcgcag caggtcgccg ccggagaggt ggttgaccgt ccggcgtccg tggtgaagga
     5341 actggtcgag aatgctctgg acgccgaagc ctcacgcatc gaggtagagc taggagaagg
     5401 cggtaccgcc agtatcctgg tgcgcgacga cggctccgga atgagcgcgg aggatgcccg
     5461 c
//
LOCUS       DASSMB010000227        11246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_319969, whole genome shotgun sequence.
ACCESSION   DASSMB010000227 DASSMB010000000
VERSION     DASSMB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319969"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="cofH"
                     /locus_tag="VFJ72_13340"
     CDS             <1..1188
                     /gene="cofH"
                     /locus_tag="VFJ72_13340"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HET7480486.1"
                     /translation="PGTIEPVPEKTVAEARGGRPSKVRGEFARALSGAGERDLSEEEI
                     ALLFTARGTELADLCRVADELRREVNGDEVSYVINRNINYTNLCYFRCRFCAFSKGPK
                     SLNLRGDPYLLDTTEVARRAREAWEKGATEVCMVGGIHGSFTGQNYIDYLRAVKDEVP
                     EMHVHAFTPLEVFQGAHTLGISVEEFLEELKDAGLGTLPGTAAEILDDEIRAIICADK
                     INTQQWADVMRAAHAVGLRATTTIMFGHVDGPVNWARHLMVLRDIQAETGGFTEFVPL
                     PFVHMGAPLYLQGRSRKGPTFAETVKIHAVGRIALHGYIDNVQVSWVKMGEEGAKLCL
                     QAGCNDLGGTLMNESISRAAGASHGQEMLPEELERIIREIGRTPRRRTTLYGTPDREY
                     SAR"
     gene            1333..2397
                     /locus_tag="VFJ72_13345"
     CDS             1333..2397
                     /locus_tag="VFJ72_13345"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7480487.1"
                     /translation="MRGRVPGLSVAVVHPKGVVWKKGFGIADLGSGSPAAPETIYPWF
                     SMTKIVTATAVAQLADRGLLSLEDPVSLHYPPFAELRPTHHAERVTIRHLLNHSAGLA
                     NPLPITWVHPADRPAPDQRAFVRGVLEKHPRLRFSPGAKGSYSNVGYLVLGEIVGSAS
                     GRPYPEYVRENILEPLGMRHTSFVYVGDASKGSFATGYQLRRSPMTWLMKLMLPDGIL
                     DGTEGRFVAFNRFYVDGAAYGGLVGPVSDAARFLQAHLAGGELEGKRILSEATAARMR
                     STLVSGGKRDFGLGWYRPREARNMRPGFVEHLGGGGGFWNCMRLYPDVPSGIVTMGNA
                     TRYDHEVIIGLLGKRRGLAR"
     gene            complement(2429..3478)
                     /locus_tag="VFJ72_13350"
     CDS             complement(2429..3478)
                     /locus_tag="VFJ72_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET7480488.1"
                     /translation="MVLSADQSAFLVRQRVARLATASASGEPHVVPVCFAYTPDSVYI
                     ALDEKPKDVPPERLKRVRNILENPKVALVADRYAEDWSQLAFVMVRGAAELLEPGSDE
                     HSAAVRLLRGKYHQYEGMRIEANPVIAIRVERVSSWGALEASEDPEPPLLDAVRGRRS
                     VRRYLPKAVPKEVVERVLEAARWAPSPHGRQPWRFAVITRQATKQRLADAMGKEWRAN
                     LRMDGQSKEVVEKRLEGSHHRLLDAPVLVLMCLYLKDLDDYPDAARQENETTMAVQSL
                     GAAAQNVLLAAYEVGLDTGWMCAPLFAPGTVTASLGLDPKLIPHALLTLGYAEGDPPK
                     RRARMPLEDLVVFRG"
     gene            complement(3490..4518)
                     /locus_tag="VFJ72_13355"
     CDS             complement(3490..4518)
                     /locus_tag="VFJ72_13355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7480489.1"
                     /translation="MRDGGAGPTFGLWYDFRQKLPLGDYARFYAECLEEIEEGERLGL
                     TGVWLSEHHFVDDGYLPSPLVVAAAIAARTSSMTIGTNVLLLPMHHPLRVAEDAAVAD
                     LVSGGRFVLGVGQGYVQHEFETLGFNRKNRPSLFQEGIEVIRQAHKQGRTGYEGKRWR
                     FDDLPFEPRPVGKLPIYIGAFADPAIDRAARLADGFLASAGGGDFGETYRKVQEALKR
                     HGREGEVFPYIASGVAFVHEDAGRAREIVAPAIAYQRTRYAEWGTDREKPRPQPVREE
                     DLPWERYLVGTPDEVAEGLIHLYQEAPYDHLCFWGRLPGVTHEQALDNMRLFAAEVAP
                     RVRAAVSA"
     gene            complement(4599..5396)
                     /locus_tag="VFJ72_13360"
     CDS             complement(4599..5396)
                     /locus_tag="VFJ72_13360"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7480490.1"
                     /translation="MIDPAPALFGLLVGFLVGLTGVGGGSLMTPFLLSIGVPAPTAVG
                     TDLTFATLTKLTGSFQHFRQRSVNLEVALFLGMGSIPAGLLGVGTLEWIKGSFDPETV
                     QSIMVTIISITLVIVGASLIYREFFMPERDEFVKPGIWDGKSPMSRRRRMYTVLFGAF
                     GGYLVGLTSIGSGSVMAVILLLLYPIAPAVIVGTDVTHAMVLSFVVGIAHMTQGNVNF
                     GLAGTLLIGGIPGVLVGSRLAPWIPGRPLKLILACMLIFVGARLLLT"
     gene            complement(5400..5972)
                     /gene="cysC"
                     /locus_tag="VFJ72_13365"
     CDS             complement(5400..5972)
                     /gene="cysC"
                     /locus_tag="VFJ72_13365"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HET7480491.1"
                     /translation="MENGTKYGRGFTLWFTGLSGAGKTTIAEIVEHELRERHGGIEVL
                     DGDIVRTNLSKGLGFSREDRDTNVLRIGFVADLLTRNGVGVIVSAISPFKEVRDQVRR
                     NIGEDFIEVFVDAPLEVCAERDVKGLYAKAFSGEIPQFTGVSDPYEAPAAPELHIKTN
                     EEEPQDSARRVIGYLEEQGYLRRARVFDRA"
     gene            complement(6007..7221)
                     /gene="sat"
                     /locus_tag="VFJ72_13370"
     CDS             complement(6007..7221)
                     /gene="sat"
                     /locus_tag="VFJ72_13370"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET7480492.1"
                     /translation="MTIKTEYTAIAPHGGELVDRRAPRDEREERKQQAEGFKKVGLGP
                     RTLSDLEMIATGVFSPISGFMVREDYESVVNTMRLSNGLVWSIPVTLSVSEDEASAIS
                     DGEEVALTDGDGRVVATMTVEDRYTYDKEHEAREVYRTDDGEHPGVAAVYRQGDVLLG
                     GEITLLEDEPNPRPFPGYYYEPAELRALFAEKGWKRVVGFQTRNPVHRAHEYIQKSAL
                     ETVDGLLLNPLVGETKSDDISAAVRMKSYETILDRYYPRNRTVLAVFPAAMRYAGPRE
                     AIFHAMCRKNYGCTHFIVGRDHAGVGSYYGTYDAQHIFDEFTAEDLDITPLFFEHSFF
                     CKACEGMGTTKTCPHDKENHVFLSGTKVREMLANGEYPPPEFSRPEVVEVLINGLRDE
                     ERTIKQPRNFAE"
     gene            complement(7218..7568)
                     /locus_tag="VFJ72_13375"
     CDS             complement(7218..7568)
                     /locus_tag="VFJ72_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480493.1"
                     /translation="MEDVRRRLPLPEEPTELKAETRSLLEESPEEGARLIGDGRFVAD
                     VVWEDWGEGLEDAGMSYERFVEISRGYAGELRLWVVGERPWDHCAAGLAGRVWRRLPA
                     RDSGKVLVASEVCR"
     gene            complement(7571..8764)
                     /locus_tag="VFJ72_13380"
     CDS             complement(7571..8764)
                     /locus_tag="VFJ72_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HET7480494.1"
                     /translation="MAGFDIRELMNDIDEAPGKVWFWDLEKAVIDADRCVQCGVCVAA
                     CPTDSIGIGDDDLPTLVKMCTGCSLCWDFCPRGGLQYESTWKIMGADHSVEGMGKVEE
                     SYTARVRQKIDGVQDGGFVSALLISLLEAGEIDGALLARESETERWKGEAFLATTPEE
                     VRECAGSFYNQTLALGHVDLKDADLPPNPRIAVVGTPCEIEGIKAMQARPWTWGSSKV
                     EAITLTIALLCTKSFNYEKLMLEEIQDKRGVDLDNVGRVDIIRGKMIVQDLDGETIFE
                     EPIRDFHGAALKGCDECADFMGHAADISVGSVGSADGYSSVLVRSEEGLAAFESVRNS
                     LELRELDKPEALDKLDALDKRVAFKSLKRAFDPEAPLFIDYEEHLENYAGTDRAPVEH
                     EAVRY"
     gene            complement(8765..10405)
                     /locus_tag="VFJ72_13385"
     CDS             complement(8765..10405)
                     /locus_tag="VFJ72_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--nitrite reductase"
                     /protein_id="HET7480495.1"
                     /translation="MQILREDQKLNKVEKIKLERHPLEVRQAIIDTYSKEGYEAMDAV
                     AGEVERLKWVGVYPQKQGGDAFMIRVKIPGGVLTPEQTKTIGQIAVDFANGPYPNPHF
                     GNNFLDITTRQDVQLHWVKMADIPKIWSRLEEVGMTTVQACGDSARNVLCCPVSGLGR
                     DEVIDAYPVAQAISDYFTGNREYANLPRKFKLSVTGCTEDCAQAEINDIGMVPARLDD
                     GTIGFNLRVGGGLSDGPRMASDIDVFVHPEEAVEISRGIAQVFGELGNRENRWTARMR
                     YLVQELGPERFREELEKRVSVDLTPAGEELTKHYRGDHVGVHEQKEEGLYYVGLNVPV
                     GRMSGEQFVEAGRLAEEYGSDVRLATDQNLIITGVREERLEELLAEPLLERFSPEPGA
                     FERGVVACTGSEFCRFAIVETKIRAVEWAKEMDERVGDVGQEAIRMHFSGCSASCAQP
                     QIGDIGFRGETAKTRDGIVEGVDIGLGGSLGKDAAFIDWVEGAKPADDVPDALVAVFV
                     QFKEERREGERFHEWARRKNNRDLREALRSAGKNRGRG"
     gene            complement(10392..11147)
                     /locus_tag="VFJ72_13390"
     CDS             complement(10392..11147)
                     /locus_tag="VFJ72_13390"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HET7480496.1"
                     /translation="MHKALRTGHNGVVVSDHEIPDIEENPEAASPQEVLRWAVERYGE
                     RLTLSVSFGGPEGMVLLDMLSRITDRVPVFTLDTGFLFDETVAFREEVMERYRVPLNV
                     LRPGLSLEEQVERYGPELWSCKPDICCEVRKVEPQRRYLRDYDAWVTGIRRDQTPQRA
                     GTPVLGHDEFFGVAKIAPLAGWSADDVEAYVHEYDVPLNPLLSMGYRSIGCEPCTRPV
                     APGEDPRAGRWADSDKTECGIHIAGGTVRRADT"
BASE COUNT         1902 a         3928 c         3480 g         1936 t
ORIGIN      
        1 ccagggacga tcgagcccgt gccggagaag accgtcgccg aagcccgcgg cgggcggccc
       61 tcgaaggtgc ggggcgagtt cgcgcgggcg ctctccgggg ccggggaacg cgacctctca
      121 gaagaggaga tagcgctgct cttcaccgct cgcggcacgg agctcgcgga cctgtgccgc
      181 gtggccgacg agctcaggcg cgaggtcaac ggcgacgagg ttagctacgt catcaaccgg
      241 aacatcaact acacgaacct gtgctacttc cgctgccgct tctgcgcgtt ctcgaagggt
      301 cccaagtcgc tcaacctgcg cggggacccg tacctgctcg acaccaccga ggtcgcgcgg
      361 cgcgcgcggg aggcgtggga gaagggcgcg accgaggttt gcatggtcgg cggcatccat
      421 ggcagcttca cgggccagaa ctacatagat tacctgcggg ccgtcaagga cgaggtgccg
      481 gagatgcacg tccacgcctt tacgccgctt gaggtgttcc agggcgcaca cacgctcggc
      541 atcagcgtcg aggagttcct ggaggagcta aaggacgcgg ggctcgggac gctgccgggg
      601 acggcggcgg agatactgga cgatgagata cgcgcgatca tctgcgccga caagatcaat
      661 acccagcagt gggccgacgt gatgcgggcg gcgcacgccg tagggctgcg agcgaccacc
      721 acgatcatgt tcggccacgt ggacggaccc gtaaactggg ccaggcacct gatggtcctg
      781 cgcgacatcc aggcggagac gggcggcttc accgagttcg tgccgctgcc gttcgtccac
      841 atgggcgcgc cgctctactt gcagggacgc tctcgcaagg gacctacctt cgcagagacg
      901 gtcaagatac acgccgtcgg gaggatcgcg ctgcacggct acatagacaa cgtgcaggtc
      961 tcctgggtca agatgggcga ggagggcgcg aagctgtgcc tgcaggccgg ctgcaacgac
     1021 ctcggcggca ccctgatgaa cgagagcatc tcgcgcgccg ccggagccag ccacggccag
     1081 gagatgctgc cggaggagct ggagcgcatc atccgggaga tcggccgcac cccccgccgc
     1141 cgcaccacgc tctacggcac gccggaccgg gaatactccg caaggtagac gggagcctcg
     1201 gcacacgccc gcgcgaggac aacccggcgc gggcacgacg aaccggcgag aagactcgtc
     1261 cggatccacg gggagggcga aatagcctcg gataccgcac gtaacccacc ggaggccggg
     1321 ataccgcgcg ccatgcgggg cagagttccg gggctttccg tggccgtcgt ccacccgaag
     1381 ggagtcgtgt ggaagaaagg gttcggcatc gccgacctcg gctcgggctc ccccgccgcg
     1441 ccggagacca tctacccgtg gttctcgatg accaagatcg tgacggcgac cgccgtcgcg
     1501 caactcgccg acagggggtt gctcagtctg gaagatcccg tctccctcca ctacccgccg
     1561 ttcgccgagc tgcggcccac gcaccacgcc gaacgggtga ccatacgcca cctcctcaac
     1621 cacagcgccg ggctcgcgaa ccctctcccc ataacgtggg tgcatcccgc cgacaggccg
     1681 gcgccggatc agcgcgcgtt cgttcgtggt gtgctggaaa agcatcccag gctccggttc
     1741 tctcccggcg cgaagggcag ctactccaac gtcgggtacc tggtgctcgg cgagatcgtc
     1801 ggttcggcgt cggggcgccc gtaccccgag tacgtgcgcg agaacatcct cgaaccactc
     1861 ggcatgcgcc acacctcgtt cgtctacgtc ggcgacgcct cgaagggctc gttcgccacc
     1921 ggctaccagt tgcggcgcag cccgatgacg tggctgatga agctgatgct accggatggg
     1981 atcctggacg ggaccgaggg ccggttcgtg gcgttcaacc gtttctacgt ggacggcgcc
     2041 gcttacggcg gcctcgtcgg cccggtctcg gacgccgcgc ggttcctcca ggcccatctg
     2101 gccgggggcg agctggaggg caaacgcatc ctctccgaag cgaccgcggc acgtatgcgg
     2161 agcaccttag tctccggcgg gaagcgggac ttcggcttgg gatggtaccg gccccgcgag
     2221 gctcgcaaca tgcggcccgg tttcgtggag cacctgggcg gaggcggcgg tttctggaac
     2281 tgcatgcgcc tctacccaga cgttccttcg ggcatcgtca cgatgggcaa cgctacccgc
     2341 tacgatcacg aggtcatcat cggcctgctc ggtaaacgtc ggggtctcgc gaggtagcgt
     2401 ccaccggcgc ggcagtcgcg ccgcggggtc agccccggaa gaccacgagg tcttccaggg
     2461 gcatgcgcgc acggcgtttc ggtggatcgc cctccgcgta acccagcgtc agtagggcgt
     2521 gcgggatcag cttcgggtcg aggcccaagg acgccgtgac cgtcccgggg gcgaagagcg
     2581 gggcgcacat ccagccggtg tccagaccaa cctcgtaggc ggcgagcagt acgttctgcg
     2641 ccgccgcgcc gaggctctga acggccatcg tggtctcatt ctcctggcgg gccgcgtccg
     2701 gatagtcgtc caggtctttg aggtagaggc acatcagcac caggaccggg gcgtcgagga
     2761 gccggtggtg ggagccttcg agacgcttct cgacgacttc cttgctctgg ccgtccatcc
     2821 ggaggttcgc ccgccactcc ttccccatcg cgtccgcgag acgctgcttc gtcgcctgcc
     2881 gggtgatgac ggcgaagcgc cacggctgcc gtccgtgcgg cgagggggcc caccgggccg
     2941 cctccagcac gcgctcgacg acctccttag gcaccgcctt cggcaggtag cgccggacgg
     3001 agcgccggcc ccgcaccgcg tcgagcaacg gcggctccgg gtcttcgctc gcctccaggg
     3061 cgccccagga cgaaaccctc tccacacgga tggcgattac cgggttcgcc tcgatccgca
     3121 tgccctcgta ctggtggtac ttgccgcgca agagcctcac agcggctgaa tgctcgtcag
     3181 agccgggttc cagaagctcc gccgccccac gcaccataac gaaggcgagc tggctccagt
     3241 cctcggcgta ccggtcggcg acgagcgcga ccttcgggtt ctccaggatg ttgcggacgc
     3301 gcttgaggcg ttcaggcggc acgtccttcg gtttctcgtc cagggcgatg tagacggagt
     3361 ccggcgtgta ggcgaaacag acgggtacca cgtgcggctc gccggaggcg ctagccgtcg
     3421 cgaggcgggc gaccctctgc cggaccagga aggcgctctg atcagcgctc aggaccatgg
     3481 gacgggcttt caagcgctca ccgcggcgcg gaccctcggc gcgacctcgg cggcgaagag
     3541 gcgcatgtta tccagcgcct gctcgtgggt gacgccgggc aaccgccccc agaagcagag
     3601 gtggtcgtag ggagcttcct ggtaaaggtg gatcagacct tcggccacct catccggcgt
     3661 accgaccagg tagcgctccc acggcaggtc ttcctcccgg accggctgcg gccggggctt
     3721 ctcccggtcc gtgccccact cggcgtagcg cgtgcgctgg taggcgatgg ccggggctac
     3781 gatctcccgc gcccttcccg catcctcgtg cacaaacgca accccggagg caatatacgg
     3841 aaacacctcg ccttctcgcc cgtggcgctt cagcgcctcc tgtaccttcc tgtacgtctc
     3901 accgaagtct ccgccgcccg ccgaggcaag aaagccgtcg gccagccggg cggcgcggtc
     3961 tattgccggg tctgcgaagg cgccgatgta gatcgggagc ttgccgacag gacgcggctc
     4021 gaacggcagg tcgtcgaaac gccagcgctt gccctcgtag ccggtgcgtc cctgcttgtg
     4081 agcctggcgg atcacctcga tcccctcttg gaagagcgag ggccggttct tgcggttgaa
     4141 ccccagggtc tcgaactcgt gctgcacgta tccctggccc acgccgagaa cgaaccgacc
     4201 gcccgagacc aggtccgcaa ccgccgcgtc ctccgccacg cgcagcggat ggtgcatcgg
     4261 caggagcagc acgttcgtac ctatcgtcat gctcgacgtt cgggcggcta ttgcggcggc
     4321 tacgaccagc ggcgacggca gatatccgtc gtcaacgaag tggtgctccg agagccagac
     4381 gcccgtgagc cccagccgct cgccctcctc gatctcctcc aggcactccg cgtagaaccg
     4441 cgcgtagtct cccagaggca acttctgccg gaagtcgtac cagagcccga aagtcgggcc
     4501 agccccgccg tccctcaaaa cgcctcctcg aaagagtctc taactccgac cagtccgtac
     4561 cagtataagg ccggcgcgga tacgcggagg gatgtccgtc aggtgaggag caggcgagcg
     4621 ccgacaaaga tcagcatgca ggcgaggatc agcttgaggg gccggccggg gatccagggc
     4681 gccaggcggc ttccgaccag gacaccgggg attccgccga taagcagggt tccggcgagg
     4741 ccgaagttta cgttgccctg ggtcatgtgc gctatcccga cgacgaagga gaggaccatc
     4801 gcgtgggtga cgtccgtgcc gactatgacg gccggagcta tcgggtagag caggagcagg
     4861 atcacggcca tcacgctgcc ggagcctatc gaggtgagtc ccaccaggta gccaccgaag
     4921 gcgccgaaca ggacggtgta catcctgcgg cggcggctca tcggggactt gccgtcccag
     4981 atccccggct tgacgaactc gtcccgctcg ggcatgaaga actcgcggta gatcagggaa
     5041 gcccccacaa taaccagggt gatggatatg atcgtgacca ttatggactg gaccgtctcc
     5101 gggtcgaagg agcccttgat ccactccagc gtcccgactc ccagaagccc cgccgggatg
     5161 ctccccatgc ccaggaacag cgcgacttca aggttcaccg accgctggcg gaagtgctgg
     5221 aaagagccgg ttagcttcgt cagggtggcg aaggtcaggt ccgtgccaac ggcggtcggc
     5281 gccggaactc caatcgagag gagaaacggc gtcatgagag agccgccccc gacgcccgtc
     5341 aggcctacga gaaaaccgac cagcaggccg aagagcgcgg gagcgggatc tatcatttct
     5401 tacgcccggt cgaagaccct ggcgcgccgc aggtagccct gctcttccag gtagccgatg
     5461 acccggcggg cgctgtcctg aggctcttcc tcgttggtct tgatgtgaag ctccggcgcg
     5521 gcgggagcct cgtaggggtc ggagacgccg gtgaactgcg ggatctcacc cgagaacgcc
     5581 ttagcgtaga gtccctttac gtcccgctcg gcgcacactt cgagcggcgc gtccacgaag
     5641 acctcgatga agtcctcgcc gatgttgcgc cgtacctgat cccggacctc cttgaacggc
     5701 gagatggccg acacgatcac gccgacacca ttgcgtgtca gcaggtccgc cacgaacccg
     5761 atgcggagga cattcgtgtc gcggtcctcc cgcgagaagc cgagaccttt cgagaggttg
     5821 gtcctgacga tgtctccgtc gagtacctcg atgccgccgt gccgctcgcg caactcgtgc
     5881 tcgacgatct cggcgatggt cgtcttcccg gcgccggata gcccggtgaa ccagagcgtg
     5941 aacccgcgac cgtacttcgt cccattctcc attaccattt actccttttt cgagactctc
     6001 ttacgcttac tccgcgaagt tacgcggctg ctttatagtc ctctcctcgt cacgcaaccc
     6061 gtttataagg acctccacga cctccgggcg gctgaactcc ggcggcggat actcgccatt
     6121 cgccagcatc tcccgaacct tcgtcccgga caggaacacg tggttctcct tgtcgtgcgg
     6181 gcaggtcttg gtagtaccca tgccctcgca ggccttacag aagaacgaat gctcgaagaa
     6241 gagcggcgtg atgtccagat cctccgccgt gaactcgtcg aagatgtgct gcgcgtcgta
     6301 ggtgccgtag tagctgccaa cgcccgcgtg gtcgcgtccg acgatgaagt gcgtgcagcc
     6361 gtagttcttg cggcacatcg cgtggaagat cgcctctctc ggtcccgcgt agcgcatcgc
     6421 cgccgggaac accgccagga cggtccggtt cctcgggtag tagcggtcca ggatcgtctc
     6481 gtaagacttc atcctgaccg ccgccgagat gtcgtctgac ttggtctcgc cgacgagcgg
     6541 gttgagcaag agaccgtcaa cggtctcaag ggcgctcttc tggatgtact catgcgcgcg
     6601 gtggaccggg ttgcgcgtct ggaagcccac gacgcgcttc cagcccttct ccgcgaacag
     6661 ggcgcgaagc tccgccggct cgtagtagta gccggggaac gggcgcggat taggctcgtc
     6721 ttccagaagc gtgatctcac cgccgaggag cacgtcgccc tgccggtaca cggcggctac
     6781 ccccggatgc tcgccgtcat cggtgcggta gacctcgcgc gcctcgtgtt ccttgtcgta
     6841 ggtgtagcgg tcctcgaccg tcatcgtggc gaccacgcgg ccgtcgccgt cggtcagcgc
     6901 cacctcttct ccatccgata tggcgctcgc ctcgtcctca ctcacggaga gcgtaacggg
     6961 gatgctccag accagaccgt tagagagccg catggtattg accaccgact cgtagtcctc
     7021 ccggaccatg aagccagaga tcggcgagaa gacgccggtc gcgatcatct ccaggtcaga
     7081 gagggtcctg ggaccgaggc cgaccttctt gaacccctcc gcctgctgct tgcgctcctc
     7141 ccgctcgtct cgcggggcgc ggcggtccac aagctcgccg ccgtgcggcg cgatcgcggt
     7201 gtactccgtc ttgatcgtca tctacatacc tccgaagcta caagcacttt tccggaatcg
     7261 cgcgccggca acctccgcca gacccggccg gcgagtccgg cggcgcagtg gtcccagggg
     7321 cgctcgccga ctacccaaag cctcaactca ccggcgtatc cgcgagaaat ctccacgaac
     7381 ctctcgtagc tcatgccggc atcttcgaga ccttcgcccc agtcctccca gactacatcc
     7441 gcgacgaacc gaccgtcgcc gatgagccgg gcgccctctt ccggagactc ttccagcagg
     7501 gaacgagtct cggcctttag ctccgtcggc tcctcgggca gaggaagcct tctgcgcacg
     7561 tcttccaaag ttagtacctc accgcttcat gctcgaccgg ggcgcgatcc gtcccggcgt
     7621 agttttcgag atgctcctcg tagtctatga agagcggagc ttcgggatcg aaggcccgct
     7681 tcaggctctt gaaggcaacc cgcttgtcca aagcgtctag cttatctaaa gcctccggct
     7741 tgtcaagctc ccgcaactcc agactgttcc gaacgctctc gaacgccgca agcccctctt
     7801 cgctacgcac gaggaccgaa gagtacccgt ccgcgctacc gacgctcccg acggagatgt
     7861 ccgccgcgtg gcccatgaag tccgcgcact cgtcgcagcc cttcagtgcg gcgccgtgga
     7921 agtcccggat cggctcctcg aagatcgtct ccccgtcgag gtcctgaacg atcatcttcc
     7981 cccggatgat gtccacccgg ccgacgttgt cgagatcgac gccgcgcttg tcctggatct
     8041 cctccagcat caacttctcg tagttgaagc tcttggtgca gagcaaggct atcgtcagcg
     8101 ttatcgcctc gactttagag gagccccacg tccacggcct cgcctgcatc gccttgatgc
     8161 cctcgatctc gcacggcgtc cccacaaccg cgatcctggg attcggcggc aggtcggcgt
     8221 ccttgaggtc cacgtgcccg agcgcgagcg tctggttgta gaagctcccg gcgcactcgc
     8281 gcacctcttc gggcgtcgtc gccaggaacg cctcgccctt ccaccgctcg gtttcgctct
     8341 cgcgggcgag cagggcgccg tcgatctcac cagcttcgag cagcgagatc agaagcgcgc
     8401 tcacgaaccc gccgtcctgc acgccgtcta tcttctgccg gacgcgggcg gtgtagctct
     8461 cctcgacctt ccccatcccc tcgacggagt ggtcggcgcc catgatcttc cacgtcgact
     8521 cgtactggag ccccccgcgc gggcagaagt cccagcacag cgagcagccc gtgcacatct
     8581 tgacgagcgt cggcaggtcg tcgtcgccga tgccgatgga gtccgtcgga caggccgcga
     8641 cgcacacgcc gcactgcacg cagcggtcgg cgtctatgac ggccttctcc aggtcccaga
     8701 accatacctt tccgggagcc tcgtcgatgt cgttcatcag ctcccgtata tcgaatccag
     8761 ccaattaacc tctacctcta ttcttaccgg cgctgcgcag cgcctcccga agatccctgt
     8821 tgttcttccg ccgcgcccac tcgtggaacc gctcgccctc acgccgctcc tccttgaact
     8881 gcacgaagac ggcgaccaga gcgtccggca cgtcgtcggc gggcttcgcg ccctcgaccc
     8941 agtctatgaa cgccgcgtcc ttgccgagcg agccgccgag gccgatgtcc acgccctcga
     9001 cgatgccgtc gcgcgtcttc gcggtctcgc cccggaagcc gatgtcaccg atctggggct
     9061 gcgcgcacga cgccgagcac ccggagaagt gcatccttat cgcctcctgg cccacgtccc
     9121 cgacgcgctc gtccatctcc ttcgcccact cgaccgcccg gatcttggtc tccacgatgg
     9181 cgaaccggca gaactcactc cccgtacagg cgaccacgcc gcgctcgaac gcgcccggct
     9241 ccggcgagaa ccgctccagg agcggctccg cgagcagctc ctccagccgc tcctcccgaa
     9301 cgcccgttat gatgaggttc tggtccgtgg cgagccgcac gtccgagccg tactcttcgg
     9361 ccagccgtcc ggcctccacg aactgctcgc cgctcatccg gccaaccgga acgttcagcc
     9421 cgacgtagta gagtccctct tccttctgct cgtgcacccc gacgtggtcc ccgcggtagt
     9481 gcttcgtcag ttcctcaccg gccggcgtca ggtcgacgga gacccgcttc tccagctcct
     9541 cccggaaacg ttccgggccg agctcctgca ccaggtagcg catgcgggcg gtccagcggt
     9601 tctcgcggtt gccgagctcg ccgaagacct gggcgatgcc gcgcgagatc tccaccgcct
     9661 cctccgggtg aacgaacacg tcgatgtccg aggccatccg cggcccgtcg gagagcccgc
     9721 cgcccacccg caggttgaag ccgatggtgc cgtcatccag ccgcgccggg accataccga
     9781 tgtcgttgat ctcggcctgc gcgcagtcct ccgtgcagcc cgtcacgctc agcttgaact
     9841 tcctcggcag gttcgcgtac tcgcgattgc ccgtgaagta gtccgagatg gcctgcgcca
     9901 ccgggtaggc gtctatgacc tcgtcgcggc cgagcccgga gacgggacag cacagcacgt
     9961 tccgggccga gtctccgcac gcctgcacgg tcgtcatgcc gacctcttcg agcctgctcc
    10021 agatcttcgg gatgtcggcc atcttcaccc agtggagctg cacgtcctgg cgcgtggtga
    10081 tgtccaggaa gttgttgccg aagtgagggt tcgggtatgg gccgttggcg aagtccaccg
    10141 ctatctgccc gatggtcttg gtctgctccg gcgtcagcac cccaccaggg atcttgacgc
    10201 ggatcatgaa agcgtccccg ccctgcttct gcgggtacac cccgacccac ttgaggcgct
    10261 cgacctcgcc ggctaccgcg tccatcgcct cgtacccttc cttcgagtac gtgtcgatga
    10321 tcgcctgccg cacctccagc gggtgccgct ccagctttat cttctctacc ttgtttagct
    10381 tctggtcttc tctaagtatc tgcacggcgt actgtgcctc ccgctatgtg gataccgcat
    10441 tcggtcttgt ccgagtctgc ccagcgtccg gcccgagggt cttcccccgg cgcaacgggt
    10501 ctggtacagg gctcgcagcc gatgcttctg tatcccatgc tcaacagcgg gttgagcggc
    10561 acgtcgtact cgtgaacgta ggcttccacg tcgtcggcgc tccacccggc gagcggagcg
    10621 atcttcgcaa ccccgaagaa ctcatcgtga ccgagaaccg gcgtaccggc gcgctgcggc
    10681 gtctgatccc gccggatacc cgtcacccag gcatcatagt cgcgcagata ccggcgctgc
    10741 ggctcgacct tccgaacctc gcaacagatg tccggcttgc agctccagag ctccggcccg
    10801 taacgctcga cctgctcctc taaagagagc cccggtctga gcacgttcaa cggcacccgg
    10861 tagcgctcca tgacctcctc gcggaaagcc accgtctcgt cgaacaggaa cccggtatcc
    10921 agcgtgaaga caggcacccg gtccgtgatg cgggagagca tatccaggag gaccatcccc
    10981 tcgggaccac cgaacgacac ggagagcgtc aaccgctcgc cgtaccgctc caccgcccac
    11041 cgcaacacct cctgcggaga cgcggcttcc gggttctcct ctatgtctgg tatctcgtgg
    11101 tcgctcacaa ccacaccgtt atgccccgtt ctcaaagctt tatgcatctc actccttctt
    11161 ctgaaagaga aaagtttcta aaattgaggg aaaaattagc tacagcagcg aacgctacat
    11221 cgaatacaca tcgccggcgc acacag
//
LOCUS       DASSMB010000228         3580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_823268, whole genome shotgun sequence.
ACCESSION   DASSMB010000228 DASSMB010000000
VERSION     DASSMB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3580
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823268"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VFJ72_13395"
     CDS             complement(<1..839)
                     /locus_tag="VFJ72_13395"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480497.1"
                     /translation="MKLRRFSGISVRNLRARPQRTLLTAIGIVLGVGIVFGVLTLSDT
                     MSGTFKNLFTRAYGAAELTVTANGGSGTFTEKTVEKVRGYDGVGSAAPRLSLPASLIL
                     PEKDANGLPKVQSMRLFGVEPKSAALATGFELTKGRFPRSGREITLDGGSAKSAGVKV
                     GEKVTVGTPEGPKKMKVVGLLRIPGGSFGGVAFGMAPLPYAQEVFDKPGKISGIAVQP
                     AEGVDAGELQRKLDRGLGTGLQAERSETRTQQVGSQLQGFRISLLFFAGTALFVGAFL
                     VFNA"
     gene            complement(843..1601)
                     /locus_tag="VFJ72_13400"
     CDS             complement(843..1601)
                     /locus_tag="VFJ72_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7480498.1"
                     /translation="MENGAGGAGIAVRTVDLGKVHGAGSMAVRALDGVSLEFPSGEFA
                     AIMGPSGSGKSTLLHLLGALDRPTSGRVVVGETDLSGLSDRKLTLLRRARMGFVFQFF
                     NLIPTLTAEENVLLPALIAGEKAAKYSGRLDELLDLVGLTARRSHRPDELSGGEQQRV
                     AIARALVRNPDIILADEPTGNLDSKTGVGVLELLRESAERYEQTILMVTHDPRAAATA
                     DRVVFLSDGRVVDEARDPDADGILERIKSLESKG"
     gene            1799..2125
                     /gene="rpsF"
                     /locus_tag="VFJ72_13405"
     CDS             1799..2125
                     /gene="rpsF"
                     /locus_tag="VFJ72_13405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7480499.1"
                     /translation="MLIVIPELDEEQVESTIGRFRTIIERTGGEAGEVNHWGRRKLAY
                     EIDHRQDGFYAVMQFTAGERTLIELKRILRVSDDILRHMIVKLPPSYAHQPLPEPELE
                     SAEASA"
     gene            2159..2410
                     /gene="rpsR"
                     /locus_tag="VFJ72_13410"
     CDS             2159..2410
                     /gene="rpsR"
                     /locus_tag="VFJ72_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015071887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET7480500.1"
                     /translation="MGKRRAPSSRRERPVKSKPCVFCKENIAYVDYKDYNVLRRFTSD
                     RGKIRARRVTGLCPQHQREAAVAIKRAREMALLAYVAGQ"
     gene            2421..2870
                     /gene="rplI"
                     /locus_tag="VFJ72_13415"
     CDS             2421..2870
                     /gene="rplI"
                     /locus_tag="VFJ72_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7480501.1"
                     /translation="MQVILLGDVEKIGQRGDIVDVSRGYVRNFLAPRGLAEVATPAKM
                     EEARRQMEEAEERDRRLAERAEEVAATLNKSVITIEARTGEDERIFGSITAANIASAI
                     ERARGIHLDRRRVKLAEPIRSLGTHQVPVQVHGDVEANVKIIVVPKL"
     gene            2991..>3580
                     /locus_tag="VFJ72_13420"
     CDS             2991..>3580
                     /locus_tag="VFJ72_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HET7480502.1"
                     /translation="MREVPTGPDAARVPPHDLDAERAVIGAMLVSETAVAAVAERLRA
                     EDFYSETHRILYRAMMKLYSNGDPIDQLTLTNELRGMGEYDRVGGRAYVFQIVESVPT
                     AANAGRYAEIIRGKSLLRAVIDAGSRIAEDAFREPDDVNEALDAAEQLIYSVSNRSLG
                     EQLSPVSELAPGALEMIQKLYEQEGEVTGVETGFDDL"
BASE COUNT          660 a         1205 c         1147 g          568 t
ORIGIN      
        1 gcgttgaaga ccaggaacgc cccgacgaag agcgcggtcc cggcgaagaa gagcagcgag
       61 atcctgaacc cctgcaactg gctcccgacc tgctgcgtcc tcgtctccga gcgctccgcc
      121 tgcaaccccg ttccgagccc ccggtctagc ttcctctgca actctccggc gtccacgccc
      181 tccgccggct gtacggcgat acccgagatc ttgccgggct tgtcgaagac ctcctgcgcg
      241 taagggagcg gcgccatccc gaaggccacc ccgccgaacg agccgcccgg tatccgcaag
      301 agcccgacga ccttcatctt cttcggcccc tccggcgtcc cgaccgttac cttctctccg
      361 accttaaccc ccgcgctctt cgccgagccg ccgtccagcg tgatctcccg gccgctcctc
      421 gggaaccgtc ccttcgtcaa ctcgaagccc gtcgccagcg ccgccgattt cggttcgacg
      481 ccgaagagac gcatgctctg gaccttcggc aacccgttcg cgtccttttc gggcagtatc
      541 agggacgccg gcagcgagag ccgcggcgcg gccgatccta ccccgtcgta gccgcgcacc
      601 ttctcaaccg tcttctcggt gaacgtcccg ctgcctccgt tggcggtcac ggtcagctcg
      661 gcggccccgt aggcgcgggt gaaaaggttc ttgaaggtgc cggacatcgt gtccgagagc
      721 gtgagcaccc cgaagacgat ccccacgccc agcacgatgc cgattgccgt aagcagcgtg
      781 cgctgcggcc gggcgcgcag gttcctcacg ctgatgccag agaagcgccg gagcttcacg
      841 gcctagcctt tcgactccag agacttgatg cgctccagga tcccgtcggc gtcggggtcg
      901 cgggcctcgt ccaccacccg cccgtccgag aggaagacca cccggtcggc ggtcgcggcg
      961 gcgcgcgggt cgtgcgtgac catcaggatc gtctgctcgt agcgctccgc ggactcgcgc
     1021 aacagctcca gcactccaac tccggtcttc gagtccaggt tgccggtcgg ctcgtcggcc
     1081 aggattatgt ccgggttgcg gacgagggcg cgcgcgatcg ccacccgctg ctgctccccg
     1141 ccggatagct cgtccggccg gtgcgaccgg cgcgccgtga gcccgacgag atccagcaac
     1201 tcgtccaacc tccctgaata cttggcggcc ttctcgccgg ctatgagcgc gggcagcaac
     1261 acgttctcct cggcggtgag cgtggggatc aaattgaaga actggaacac gaagcccatt
     1321 cgggcccgcc gcaagagcgt cagcttcctg tcagataacc ccgataggtc cgtctctccg
     1381 acgacgaccc tgccggaagt aggtcgatcc agcgcgccga ggaggtgtag tagcgtgctc
     1441 ttgcccgacc cgctcggacc catgatcgcc gcgaactcgc cgctcgggaa ctccagagac
     1501 accccgtcca gcgcccgcac cgccatcgag cccgcgccgt ggaccttccc gaggtccacc
     1561 gtcctgaccg cgataccggc tccgcctgct ccgttctcca cccgctgagt atatatccgg
     1621 gcgtaggtct aaaggtgaaa gaaaggtgaa agcggcgaca ccatgcgtaa gaccgtttgt
     1681 aacacctcgc tctcgctgct aaactattcc ggcaatccaa taggcccctg cttcgacggg
     1741 atacaccctc gaagccgatt ccagaccgga ggaggcaaac atcgacactt acgaagccat
     1801 gctcatagtc atcccggagc tcgacgagga gcaggtcgag agcaccatcg ggcgctttcg
     1861 cacgatcatc gagcgcacgg gcggcgaggc cggggaagtc aaccactggg gacgccgcaa
     1921 gctggcttat gagatagacc accgtcagga cggtttctac gcggtcatgc agttcaccgc
     1981 gggggagcgg accctgatcg agttgaagcg catcttgcgc gtctcggacg acattctgcg
     2041 gcacatgatc gtgaagctcc cgccgagcta cgcgcaccag ccgctgccgg agccggagtt
     2101 ggagtccgcc gaggcgtcgg cctagatcct ttagagcatt tcaggagagg agatttagat
     2161 gggtaagagg agagctcctt cttctcgcag ggagcgcccg gtcaagagca agccgtgcgt
     2221 cttctgcaag gagaacatag cgtatgtgga ctacaaggac tacaacgtgc tgcgacgctt
     2281 cacgtcggac cgcggcaaga tccgtgcccg ccgcgtaacc gggctctgcc cgcagcacca
     2341 gcgcgaggcc gctgtcgcga taaagcgggc ccgcgagatg gctcttctgg cttacgtggc
     2401 cggacagtag ggggtatctg atgcaggtca tccttttggg agacgtcgag aagatcggtc
     2461 agcgcggaga catcgtggac gtgagccgcg gctacgtgcg caacttcctg gccccgcgcg
     2521 gtctcgccga ggtggcgacg ccggcgaaga tggaggaggc ccgccgccag atggaggagg
     2581 ccgaggagcg cgaccggcgc ctggccgaac gcgccgagga ggtcgccgcg acactcaaca
     2641 agagcgtcat caccatcgag gcgaggacgg gcgaagacga gcggatcttc ggctccatca
     2701 ccgctgcgaa catcgcctcc gccatcgagc gggcgcgtgg gatccacctg gaccgccgcc
     2761 gcgtgaagct cgccgaaccg ataagatccc tcgggaccca ccaggtcccc gttcaggtcc
     2821 acggagacgt cgaagccaac gtaaagatca tcgtcgtccc caaactctag agagcagcct
     2881 gtagctcgac tgtagcttca ggcacaacct gaaccaaaag gtaaagggtc ccgtcttgta
     2941 acggggccct ttatgctgca tgctaggaga ttatggagcg acgggggagg atgcgggagg
     3001 tacccaccgg gccggacgcg gctcgcgtac cgccgcacga cctggatgct gaaagggccg
     3061 ttataggggc gatgctcgtc tccgagacgg cggtcgcggc ggtggcggag aggctgcggg
     3121 cggaggactt ctactccgag acgcaccgca tcctgtaccg ggcgatgatg aagctctatt
     3181 cgaacggcga cccgatagac cagctcacgc tcaccaacga gctacggggg atgggcgagt
     3241 acgaccgcgt cggggggcgg gcgtacgtgt tccagatcgt cgagagcgtg ccgacggcgg
     3301 cgaacgcggg ccggtacgcg gagatcatcc gcggcaagtc gctgctgcgg gccgtaatag
     3361 acgcggggag ccgcatcgcc gaggacgcct tccgcgagcc ggacgacgta aacgaggcgc
     3421 tcgacgcggc ggagcagctc atctacagcg tctccaaccg ttcgctcggc gagcagctct
     3481 cgccggtgtc ggagctcgcg ccgggggcgc tggagatgat ccagaagctc tacgagcagg
     3541 agggcgaagt aaccggcgtc gagaccggct tcgacgacct
//
LOCUS       DASSMB010000229        13862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1460307, whole genome shotgun sequence.
ACCESSION   DASSMB010000229 DASSMB010000000
VERSION     DASSMB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13862
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1460307"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFJ72_13425"
     CDS             <1..499
                     /locus_tag="VFJ72_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480503.1"
                     /translation="ATVEVCDLDPSARLAAAHLGFATPGLEDGLSRADVVFTATGRDG
                     AIPFDALFACKSGAFLANVGHTSGELPVDELRAHITDTPRSFVERCDVDGKTLYLLAG
                     GAMFNLAAGQGVPYDTFDLVTALMLEAMAFLVNEGVGYPPGLHPLPKKVQDRAARLYL
                     GEAVG"
     gene            complement(503..700)
                     /locus_tag="VFJ72_13430"
     CDS             complement(503..700)
                     /locus_tag="VFJ72_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480504.1"
                     /translation="MEQPKSEAEVALELMRLVLDQAGSRRLSPTRTLALYHLCKEAVA
                     YSPEGSVTADYLEESLKKLLQ"
     gene            821..1486
                     /locus_tag="VFJ72_13435"
     CDS             821..1486
                     /locus_tag="VFJ72_13435"
                     /inference="COORDINATES: protein motif:HMM:NF021501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HET7480505.1"
                     /translation="MGELRQAYTRCPICSHVFRAPSSGPHVTVGRESDLCPKVPGRAS
                     DAARLIQSDLTMCPACSFAAAGSFEDLDLTFDERHGIEERLREDGLLRVFRGSPPPWL
                     AFHSAEICGKERGLRARDLGDLCLRASWVCRKEREQPFESTFQLRAIRHFIRSLEQDA
                     LVGRELSVTTYLVGELNRRLGNHREALNWYVNAERTLEGDNLAWLGRLISRQRELAKE
                     QAA"
     gene            complement(1489..2322)
                     /locus_tag="VFJ72_13440"
     CDS             complement(1489..2322)
                     /locus_tag="VFJ72_13440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7480506.1"
                     /translation="MKVYRSGRRPGRERERRDGRLRPVLLVLLALCVLFLAYLLLPIG
                     GNRAVLLGSDARADEASRSDTIVVAKAGGGMLAVPRDTLVEIPGVGQDKINAAFANGG
                     PELTVETLEKLTGLSIGNYVVVDFGGVEEIVNALGGITIDVEEPISYTLDGRPVSIPA
                     GTQELDGPQALGYVRYRGDPTADIGRIGRQQRFLQALATEATSPSKLLRLPATARAVW
                     RNIDTNMNPIAALRFAIRMRLADMGEAELYPGTPKYIDGISYWVPDKDAGREVVTRTV
                     E"
     gene            2498..2668
                     /locus_tag="VFJ72_13445"
     CDS             2498..2668
                     /locus_tag="VFJ72_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480507.1"
                     /translation="MTEEEIARFDLVLREADRIRREVMMEEVLYVTDEVAPAHVIYGL
                     DRIKEDVLLFAA"
     gene            2774..3139
                     /locus_tag="VFJ72_13450"
     CDS             2774..3139
                     /locus_tag="VFJ72_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480508.1"
                     /translation="MRQQREQNVLGSVRVLVAMEPQSYREVIGYAIQGLRPNIAVSVA
                     DPRMLAAEVARLGPEMVIYSSPGEPPAGITTWLEVPENPALPATLYQEGRRSELPEFD
                     LPILLAVLDNAHLGTHQVA"
     gene            3162..3635
                     /locus_tag="VFJ72_13455"
     CDS             3162..3635
                     /locus_tag="VFJ72_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480509.1"
                     /translation="MIQVALYSVTPVMEPQETRAAFERLTSGTKRVLLVGSCVWFREA
                     LAYPINRDPGLFVVQQAGSLEGLREDAVDHDVILTDLDPLLHREPDIVSRLSDCSFRA
                     PLLALTGSPDLGVLLDALEGGADMLLSKSASVAEIISSVKLLASTGATRTRRGRS"
     gene            complement(3676..3930)
                     /locus_tag="VFJ72_13460"
     CDS             complement(3676..3930)
                     /locus_tag="VFJ72_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480510.1"
                     /translation="MPAPRDIPDDAPGRIPPGEGYELYRHRNAANMKTRNWHGLYWAR
                     RTAEGEYEIRSVPTMLGEPSAPGGKMPGARFERLYERVEI"
     gene            complement(3956..4324)
                     /locus_tag="VFJ72_13465"
     CDS             complement(3956..4324)
                     /locus_tag="VFJ72_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480511.1"
                     /translation="MKRILIVEDHLSFRQALAKLFEWNTAFEDDVQTSTLAESRRRIG
                     RMDGFEVAIIDLGLPDGDGTDLIRDLRATDPEVPILVLTQSTDPARHDRALGAGADKV
                     LTKGVSVEEILGAVRELDKI"
     gene            4470..5318
                     /locus_tag="VFJ72_13470"
     CDS             4470..5318
                     /locus_tag="VFJ72_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480512.1"
                     /translation="MSILTVMEKLEAKSFDIGEERVAWEGLASACSPPFRRLAAFLLV
                     GFTFFIAITTAVVLFYNLFGERLIEVQGVAVPERAFYTTMILSLGLALGAYLFWLAWS
                     LRAYSSFADVLRRGGLDPKRPTVGGLRAYSDEQLLALRSRYEKTANQKLKARLGRTFG
                     FHENDSFALAPLSVLPGTFEMNALRVEWELNLILREAEPMPEVSWWTEGRNAILPRRA
                     DEIRKLLYALRYTGDSVRELKRRYGYRPDRWHATVPEGKLWDAVRDYESARRIQAALG
                     RKSRVG"
     gene            5371..6459
                     /locus_tag="VFJ72_13475"
     CDS             5371..6459
                     /locus_tag="VFJ72_13475"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET7480513.1"
                     /translation="MEARFGATPPYTVGVEEEFQLVDSGSRELSPAVDGVLAAGKDSK
                     LITSELSQSCVEMLSPVFGTVADLSRELPGLRREVRRFASEGGVEIASAGTHPFSDPT
                     GQPLNQSEHHRTVEKEMGWVARTQAIYGLHVHVAVPDGETAIRAVGALSRYVPLLIAL
                     ASNSPFWRGEDTRLASTRIKIFEIFPRSGLPPAFRNWKHFERHIETLVASGSIPDYSW
                     CWWDVRPHQRFGTVEVRALDAQTDPACTTSLTALIQCVVATSRDHAPEDPLLTAENKW
                     RAARFGMDATFYDFDARREINARALARSLVERLRPVSQDLGCEEELAGVLKIVDHGTG
                     AELQRAAFGRTGSLQGVVEYLVSGTAAE"
     gene            6494..8161
                     /locus_tag="VFJ72_13480"
     CDS             6494..8161
                     /locus_tag="VFJ72_13480"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET7480514.1"
                     /translation="MGKVPGMAPSLILGPLLRYAGASDATVWVETDAACEVEVLGHRA
                     RTFAVEGHHYALVLITDLEPGETYEYAVALDDAQVWPESDSSFPPPVIRTIAPESDLT
                     LAFGSCRVAMPQEPPYTLTRDEDSNGYERDALRALALRMMREPRESWPDVLLMLGDQV
                     YADEVSPGTREFIRSRRDPDEGPGETVADFEEYTRLYWESWKDPVVRWLLSTVPSAMI
                     FDDHDVHDDWNTSGTWVRQMRSKWWWEERIVGAFVSYWIYQHLGNLSPRELDEDGLFN
                     RVREADDATRILREFAYRADREVAGTRWSYHRDFGNVRLLMIDSRAGRVLEEGRRFMV
                     DEEEWAWIVDKADGDFDHLLFGTSLPLLMAPGLHRLEAWNEAVCAGAWGNLAARAGEF
                     LRQLIDLEHWSAFQKSFEALADLLRSVAAGERSGGDPPASIVVLSGDVHHGYLAEALF
                     GEEAKSPVYQAVCSPMRNPLGRPERIAMRFGWSKLGERIGMALTRLAGVEEPSFRWHM
                     KHEEPWFDNQVSTLRLEGRRATLECEKTSPDDPEDPQLHDVFHHRLA"
     gene            8221..8817
                     /locus_tag="VFJ72_13485"
     CDS             8221..8817
                     /locus_tag="VFJ72_13485"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HET7480515.1"
                     /translation="MAEHRGAGFFEALQLDPVIPAVHAEGEPLEKALSEDHPAIFVLG
                     GDIFKLTHRIEGEKRRPPVFVNVDLIGGIAGDKTGIAFLSRHVEGVISTNRNVIEMAN
                     STALITVQRLFGLDAMAIERGLRLVKRAKPQCVEILPALSCPPMVSRYPEVLNRPVLA
                     GGLLKSREEVMSVLNAGALGVSTSALDLWSIGRLTRSS"
     gene            9199..10761
                     /locus_tag="VFJ72_13490"
     CDS             9199..10761
                     /locus_tag="VFJ72_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET7480516.1"
                     /translation="MAEGPYLIGIDGGTGGVRAGIFDREGTALIFNGVEHGTKFPRSG
                     WAEQDPDDWWSCLVQAVRGAMEESGVAPEEIAGISTDATTCTVVAMDAQNRVLRPALL
                     WMDVRASDQARRIQETGDPALKYNGYGAVSAEWMPSKALWIKENEPETYNEAARICDY
                     ADWMTFRLTGEWTASVNTAAFRAYYDRDAGGWPESLYSAIGLEDVLEKFPPKVLDMGT
                     AVGGLTKEAADELGLPEGIPIAQGGGDAFVGTLGLGVTEPGKIAFITGSSHVIVGQAA
                     EPHYGRGFFGSYTDGIVPGQYSVEGGQVSTGSIVAWFKNSFAGDAAAEAERRGVDPYL
                     VLNEMAAEVPIGSNGLIVVDYFQGNRTPYTDSLARGMIWGLSLGHGTGHIFRAILEGI
                     CYGTEHIFRTMRQNGFQPGEAVAAGGPTKSELWMQMHADVSNVPISFTRESEGPVLGC
                     AMLASVGAEIYPDIQTAADNMVHTERTIEPNPEAHEAYQFYVDRYVETYPRMRELMHK
                     VVGHEAAHGHMQ"
     gene            10883..11761
                     /locus_tag="VFJ72_13495"
     CDS             10883..11761
                     /locus_tag="VFJ72_13495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET7480517.1"
                     /translation="MADQAGAGSTGTLLLPDRLYKGYVFDLDGTIYLGDELLPGAKRL
                     ILELRERGKKVVFLSNNPTKDPEMYAEKLTKLGLPTPQEETVNTVVTMTQWLLQNHPD
                     ATVFPIAEEPLKRSLRNAGIKMSEAPEEIDIVIASYDRTFEYRKLQIAFDAIWFHKRA
                     RLVTTNPDKYCPFPGGRGEPDAASIVAAIEACTGAKCEVNTGKPDPIMLETIMGMIGM
                     DASECVMTGDRLYTEIRMALDAGMPSAIVLTGETTTEMLAAESEENKPDYVLDRIDRL
                     IPQDIWDELGWTEEDE"
     gene            11783..13153
                     /locus_tag="VFJ72_13500"
     CDS             11783..13153
                     /locus_tag="VFJ72_13500"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-1-phosphate dehydrogenase"
                     /protein_id="HET7480518.1"
                     /translation="MSAERINAALRDATDTQTVEIGPGALATVAEVFEQSFGDRTAIV
                     VTDENIWAVAGEEVQRNLEAAGRDTAEPYIFPGKPTLYAGYDNIQILVEALRPQDAIP
                     VAVGSGTLNDIVKRAAYELERPYMNVATAASMDGYTAFGASIEKDNKKQTLTCPAPRA
                     VVGDVEVLVNAPERMTASGYADLLGKVTAGADWLVADALGVEPISQKVWSLVQDSLRD
                     WTERPAELRGGDQAAMDGLIEGLILSGLAMQSHQSSRPASGAEHQFSHLWEGEGLGRD
                     LDPPLSHGFKVGVGSIAIAALFERVLERDLSNIDVDALCASWPAWEDMEKSVREAYSG
                     SKLEQAAVNETQAKYIDADTLRERLERLREVWPELRGKVEEQLMPAGELREQLRAAGC
                     PTSPEEIGLSREEFKATYFRAQMLRRRYTILDLALETGILDECVEELFAPDGFWASDT
                     ASQEIR"
     gene            13165..13641
                     /locus_tag="VFJ72_13505"
     CDS             13165..13641
                     /locus_tag="VFJ72_13505"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET7480519.1"
                     /translation="MTKKIGIGADDAALELKATLVEHLKEKGYEVEDYSPRSAEEAQA
                     AGVDYPDVAVSVAEAVARGDHDRAILCCGTGIGVNITANKVPGVRAAQCHDVFSAERA
                     RKSNNAQILTMGQRVIGPELAKTITDAWLESEFGGGGSTRKVEKMEEVDRRYHAAG"
BASE COUNT         2522 a         4537 c         4546 g         2257 t
ORIGIN      
        1 cgcgaccgtg gaggtgtgcg acctcgaccc ctccgcccgg ctcgccgccg cccacctcgg
       61 cttcgccacc ccaggactcg aagacggcct ctcccgcgcc gacgtggtat tcaccgccac
      121 cggccgcgac ggcgcgatcc cctttgatgc gctcttcgcc tgcaagagcg gcgcgttcct
      181 ggcgaacgtc ggccacacca gcggcgagct gcccgtagac gagttgcggg ctcatataac
      241 agacacaccg cgctccttcg tcgagcggtg cgacgtggac ggaaagaccc tctacctgct
      301 ggccgggggc gcgatgttca acctggccgc cgggcagggc gtcccctacg acaccttcga
      361 cctcgtaacc gccctgatgc tcgaagcgat ggccttcctc gtaaacgagg gcgtgggcta
      421 tccgccgggc ctgcacccgc tgccgaagaa ggtccaggac cgggctgcgc ggctctatct
      481 cggcgaagca gtgggctgag agctactgaa ggagcttctt gaggctctct tcaaggtagt
      541 cggcggttac ggagccttcg ggagagtagg cgacggcttc cttgcagagg tggtagaggg
      601 cgagggtgcg agtcggggag aggcgtcggc tcccggcctg atccaggacc aggcgcatca
      661 gctccagggc tacttcggcc tcgctctttg gctgctccat ggacccattc tataccggcc
      721 ggcggatgac gctatggtag acttgagcgt actttgatcc tcgtaagctc aagatccttg
      781 acgaccgtgg aggagaggct ccgcagggtg aaaggcgttc gtgggggagc ttaggcaggc
      841 ttacacccgg tgtcccatct gcagccacgt gttccgcgcg ccgtcttccg gcccgcacgt
      901 gaccgtcgga cgcgagagcg acctctgtcc caaagtcccc ggccgcgcct ccgacgccgc
      961 gcgcctcatc cagagcgatc tgacgatgtg cccggcctgc tccttcgcgg ccgccggcag
     1021 cttcgaggac ctggacctga ccttcgacga gcgccacggc atagaggagc ggctgagaga
     1081 ggacggtctg ctgcgcgtct tccgcggcag cccgccgccg tggctcgcct tccactcggc
     1141 cgagatctgc ggcaaggagc gcggcctccg cgcccgggac ctcggagacc tctgcctccg
     1201 cgcctcgtgg gtctgccgca aggagcgcga gcaacccttc gaaagcacct tccagctccg
     1261 cgccatccga cacttcatcc gctccctgga gcaggacgcc ctcgtcgggc gcgagctctc
     1321 cgtgaccacc tacctggtcg gagagcttaa ccgccgcctc ggcaaccacc gcgaggcgct
     1381 caactggtac gtcaacgccg agcgcaccct cgaaggcgac aacctcgcct ggctcggccg
     1441 cctcatctcc cgccagagag agctggcgaa ggaacaggcc gcctagagct actccaccgt
     1501 ccgcgtaacg acttcccgac ccgcgtcctt gtccgggacc cagtaggaga tgccgtctat
     1561 atacttcggg gtgccgggat agagttcggc ctctcccatg tcggcgagcc gcatcctgat
     1621 ggcgaaccgg agcgccgcga tggggttcat gttcgtgtct atgtttcgcc agacggcccg
     1681 cgccgtcgcc gggaggcgga ggagcttcga gggggatgtg gcctctgtgg cgagcgcctg
     1741 caggaacctc tgctggcggc cgatgcgccc gatgtccgcc gtagggtctc cccggtagcg
     1801 gacatagccc agggcctgcg gaccgtccaa ctcctgggta ccagccggga tggagacggg
     1861 gcggccgtcg agggtgtagg agatgggctc ctcgacatct atcgtaatgc cgccgagggc
     1921 gttcactatc tcctctaccc cgccgaaatc aactacgaca tagttgccga tggagaggcc
     1981 ggtcagcttc tccagcgtct cgacggtcaa ctccgggccg ccgttggcga aggcggcgtt
     2041 gatcttgtcc tgcccgacgc cggggatctc gacgagcgta tcgcgcggca ccgcgagcat
     2101 gccgccgccg gccttcgcca cgactatcgt gtccgagcgc gaggcttcgt ccgcgcgggc
     2161 atcgctcccg agcaggaccg ccctattgcc tcctatcggc agcagcaggt acgcgaggaa
     2221 gaggacgcac agcgctagaa gtaccagcaa caccgggcgc aggcgtccgt cccgccgctc
     2281 ccgctcgcgt ccgggacgcc gtcccgacct gtagaccttc atgcccatgc ggccattcta
     2341 tatagaaagg ctgtgagagg ccgaagccaa taatgattag atttctctac tccatttgga
     2401 ctaagcaaag atcatcttaa ttcgtgatgc ggtattccgg gccgggagag aagatatcgg
     2461 tataagaact agaaaagaac ccggaaggag gagagccatg acggaagaag agatcgcccg
     2521 gttcgatctg gtgctcaggg aggcggaccg aatccgccgg gaggtgatga tggaagaagt
     2581 cctctacgtc acggacgaag tcgctccggc acatgtgata tacgggttgg atcgcatcaa
     2641 ggaggacgtg ttgctcttcg cggcctgatc cgcataacgt aaacagcctg caagtcatcg
     2701 agtcaagaac cagcgctcta caacggtcgc ctgtccggcc cggacgtgag catggagatt
     2761 cagaggagga agcatgagac aacagagaga gcagaacgtt ctgggaagcg taagagtgct
     2821 ggtggccatg gaaccgcagt cttacagaga ggtcatagga tacgccatcc aggggctgcg
     2881 tccgaacatc gcggtctccg tcgcagaccc gaggatgctc gctgcggagg tagcgcggct
     2941 gggaccggag atggtgatct acagcagtcc cggcgagccc cctgccggta tcaccacctg
     3001 gctggaggta cccgaaaacc cggcgctacc ggcgacgcta taccaagaag gccgccgctc
     3061 ggagctgccc gagttcgacc tgcccattct gctggcggtg ctggacaacg cgcacctcgg
     3121 cacccaccag gtcgcctgag acgaacgagc gacgtgtccc gttgatccag gtcgctctct
     3181 actccgtaac tcctgttatg gagccccaag agacacgagc ggcgttcgaa cgtctgacct
     3241 ctggaacgaa gcgcgtcctg ctcgtgggga gttgcgtctg gttccgcgaa gctctggcgt
     3301 atccgatcaa ccgggacccc ggcctcttcg tcgtccagca ggctggctct ctcgaaggac
     3361 tccgcgaaga tgcagtggat cacgacgtga tcctgacaga cctggacccg ctcctccatc
     3421 gggaaccgga catcgtctcc agactcagtg attgcagctt ccgcgccccg ttactggcgc
     3481 ttaccggcag cccggacctc ggggtccttc tcgacgctct cgaaggcggc gccgacatgc
     3541 tcctctcgaa atccgcctcc gtagcggaga tcataagctc cgtcaaactc ctcgcgtcca
     3601 ccggcgcaac gcggacgcgg cgcggccggt cgtgagtccg ggcttgatca ttcggctcac
     3661 gcgcctctaa agcctctaga tctccaccct ctcgtataac ctctcgaacc gcgctccggg
     3721 catcttgcct cccggcgccg aaggctctcc tagcatggtc gggacgctcc ggatctcgta
     3781 ttctccctcc gccgtgcgcc gggcccagta gagcccgtgc cagttccgcg tcttcatgtt
     3841 cgcggcgttc ctgtgccggt agagttcgta cccttcgccg ggcgggatcc ggcccggagc
     3901 gtcatccggt atgtctcgcg gcgcgggcac tgcggctcct ctctccctct ccgtcttata
     3961 tcttatccaa ttcgcggacc gcgccgagga tctcctcaac cgagacccct ttcgtcaaca
     4021 ccttatccgc cccggcgccg agtgcgcggt catgacgtgc gggatcggtg ctctgcgtca
     4081 gcaccaggat cggcacctcc gggtccgtgg cgcgcaggtc cctgatcagg tccgtcccgt
     4141 ccccgtccgg cagtccgagg tctataatgg cgacctcgaa gccgtccatc cggccgatcc
     4201 gcctccggct ctccgcgagt gtggaggttt gcacgtcgtc ctcgaacgcc gtgttccact
     4261 cgaataattt tgccagagcc tgccgaaagc tgaggtggtc ctcgacgatc aaaatccttt
     4321 tcatctccgt cccttccaga ctgcatggtc atgcgatttc acatcctcgc agcctgaaag
     4381 ctctcgcaca tcacgcaaat ggactatttt cgcggggtcc tatgtccgcc tcgggccgtg
     4441 atctctaacc caacctcatc gggcgggatg tgagtatact gaccgtcatg gagaagctgg
     4501 aagcaaagtc tttcgacatc ggcgaggagc gggtggcctg ggagggtctg gcctccgcgt
     4561 gttcgccccc cttccggcgc ctcgcggcct tcctgctcgt cgggttcact ttctttatcg
     4621 cgatcaccac ggccgtcgtc ctgttctaca acctcttcgg agagcggctg atcgaagttc
     4681 agggcgtcgc ggtccccgaa cgggctttct acaccaccat gatcctgagt ctcggcctcg
     4741 cgctcggcgc gtacctgttc tggctggcgt ggagcctgcg cgcctactcc tctttcgcgg
     4801 acgtgctccg ccggggcggc ctggacccga agcggccgac cgtcggcggc ctgcgcgcct
     4861 actccgacga gcaactgctc gcgctgcgat cccgctacga gaagactgcg aaccagaagc
     4921 tcaaggcgcg cctcggacgc accttcgggt tccacgagaa cgactccttc gcgctcgccc
     4981 ccctgagcgt gctgcccggc accttcgaga tgaacgccct gcgggtcgag tgggagctaa
     5041 acctgatcct ccgcgaggcg gagccgatgc cggaggtctc ctggtggaca gagggccgga
     5101 atgcgatcct cccccgccgg gccgatgaga tccgcaagct cctctacgcc ctgcgctata
     5161 ccggcgactc tgtacgcgag ctgaagcgcc gctacggata ccgccccgac cgctggcacg
     5221 ccaccgtccc cgaaggcaaa ctctgggacg ccgtccgcga ctacgagagc gcccgccgca
     5281 ttcaggccgc cctcggccgc aagtcccgcg tcggatagcg ctcgcttgct gacgctggca
     5341 atcccttccg ccgctagcta gagtagctag atggaggcca gattcggcgc gacgccgccg
     5401 tacaccgtcg gggtcgagga ggagttccag cttgtggact ccggctcgcg cgagctgtct
     5461 ccggccgtgg acggcgtgct cgccgccggt aaagattcga agctcatcac ctcggagctc
     5521 tcccagtcgt gcgtggagat gctctccccg gtcttcggca ccgtggcgga cctctcccgc
     5581 gagcttccgg ggctacgccg cgaggtccgg cggttcgcga gcgaaggcgg ggtcgagatc
     5641 gcctccgccg ggactcaccc tttctcggac ccgaccggac aaccgctgaa ccagagcgag
     5701 caccatcgaa ccgtcgagaa agagatgggc tgggtagcga ggacgcaggc gatctacggc
     5761 ctgcacgtac atgtcgcggt accggacggg gagacggcaa tccgggcggt aggggcgctc
     5821 tcgcggtacg tgccgctcct tatagcgctg gcctccaatt ctcccttctg gcgcggcgag
     5881 gacacccggc tggcttccac caggatcaag atattcgaga tctttcctcg ctccggtctg
     5941 ccgccggctt tccgtaactg gaagcatttc gagcgtcata tagagacgtt ggtcgcctcc
     6001 ggcagcatcc cggattactc gtggtgctgg tgggacgtgc gtccccacca gaggttcggt
     6061 accgtcgagg tccgcgccct ggacgcgcag acggaccccg cctgcaccac gtctctcacg
     6121 gcccttatcc aatgcgtcgt agccacctcc agagaccacg ctcccgaaga cccgctgctc
     6181 accgccgaga acaagtggcg cgcggcgcgc ttcgggatgg atgcgacctt ctatgacttc
     6241 gacgccagaa gagagatcaa cgcccgcgcg ctcgcccggt cgctcgtcga gaggctgcgt
     6301 cccgtctccc aggacctcgg ctgtgaagaa gagcttgcag gggtgttgaa gatagtggac
     6361 cacggcaccg gcgcggagct tcagcgggcg gcgttcggga ggaccggatc tctccagggc
     6421 gtggtcgaat acctcgtctc cggcaccgct gcggagtagc ccgtctcgcc gggctggtgc
     6481 gaggtagcgg aacgtgggta aagtacccgg catggccccc agcctcatac tcggtcccct
     6541 cctacgctac gccggtgcat cggatgccac cgtctgggtc gagacggacg ccgcctgcga
     6601 ggtcgaggtc ctgggccacc gcgcccgcac gttcgcggtg gagggccacc attacgctct
     6661 cgtcctcatc acggacctgg aacccggcga gacttacgag tacgccgtag ccctggacga
     6721 cgcgcaggtc tggccggaga gcgacagcag cttcccgccg cccgtcataa ggaccatagc
     6781 gccggagagc gacctcaccc tggccttcgg ctcgtgccgc gtcgccatgc cgcaggagcc
     6841 gccgtacacc ctcacccgcg acgaggacag caacggctac gagcgcgacg cgctccgcgc
     6901 cctcgccctg cgcatgatgc gcgagccccg ggagagctgg ccggacgtgc tgctgatgct
     6961 cggggatcag gtctacgctg acgaggtctc gcccggaacc cgcgagttca ttcgctcgcg
     7021 ccgcgacccg gacgaaggtc ccggcgaaac ggtcgcggac ttcgaggagt acacgcggct
     7081 gtactgggag tcctggaagg acccggtcgt gcggtggctc ctctcgacgg tgccatcggc
     7141 catgatcttc gacgaccacg acgtgcatga tgactggaac acctccggga cgtgggtgcg
     7201 ccagatgcgg agcaaatggt ggtgggagga gcgtatcgtc ggagcgtttg tgtcttactg
     7261 gatctaccag cacctcggca acctctcccc gcgcgagttg gacgaagacg gcctcttcaa
     7321 cagggtccgc gaagcagacg acgcgacccg catcctgcgg gagttcgcct accgggccga
     7381 ccgcgaggtg gccggcaccc gctggagcta ccaccgcgac ttcggcaacg tccgcctcct
     7441 gatgattgac tcccgagccg ggcgggtgct ggaagaaggc cgccgattca tggtggacga
     7501 ggaggagtgg gcctggatcg tcgacaaggc cgacggagac ttcgaccatc tcctcttcgg
     7561 cacctccctc cccctcctca tggcccccgg cctgcaccgc ctcgaagcct ggaacgaggc
     7621 cgtctgcgct ggcgcgtggg gaaacctggc ggccagggcc ggagagttcc tacgccagct
     7681 aatagacctg gagcactggt cagcctttca gaaatccttc gaggcgttgg ccgacctcct
     7741 ccgctccgtg gccgccgggg agcgctcggg cggcgacccg ccggcctcca tagtcgtcct
     7801 ctccggcgac gtgcatcacg gctacctggc cgaagccctc ttcggggagg aggcgaagag
     7861 ccctgtctat caggctgtct gctccccaat gcggaaccct ctcggacggc ccgaaaggat
     7921 cgccatgcgc ttcggctggt cgaagctcgg ggagcgcatt ggcatggccc tgacacgcct
     7981 cgccggggta gaggaaccct ctttccggtg gcacatgaag cacgaagagc cgtggttcga
     8041 caaccaggtc tccaccttaa ggctcgaagg ccggagagcc actctggagt gtgagaagac
     8101 cagccccgac gacccggaag acccgcaact gcacgacgtc ttccaccaca ggctcgcctg
     8161 ataccggcga tgagatttgg cagcaagaag tattaacggt aacataggtt tatgggaaag
     8221 gtggcggaac acaggggcgc cggtttcttc gaggcgctac agctggaccc cgtgatcccc
     8281 gcggtccacg ccgaggggga gccgctggaa aaggccctct ccgaggacca cccggcgatc
     8341 ttcgtgctgg gcggagacat cttcaagctc acccacagga tcgagggcga gaagcgccgg
     8401 ccaccggtat tcgtcaacgt ggacctgatc ggcgggatcg cgggcgataa gaccggtatc
     8461 gcgttcctct cccggcacgt ggagggtgtc atatccacga accgcaacgt catcgagatg
     8521 gcgaacagca ccgccctgat tacggtccaa cgccttttcg ggctggacgc catggcgatc
     8581 gagcgcggcc tgaggctcgt caagcgggcg aaaccccagt gcgtggagat actccccgcc
     8641 ctgtcgtgcc cgccgatggt gtcccgctac cccgaggtgc taaaccggcc ggtccttgcg
     8701 ggcgggcttc tcaagagccg ggaagaggtg atgtccgtac taaacgccgg cgctctaggc
     8761 gtctccacca gcgccctgga tctctggagt atcggacgct tgacccggag ttcctagccg
     8821 gatatagtgc cataaaatac aaatcgtagc gtggcacgga gatattgatg gcctcgcacg
     8881 agaccgcgag tttccgtctc agggcgggac atggttttgt gcgaggcctt ttcgctgtcg
     8941 gagtaggagg ctctgcggga tctccgggga aggtgccggg catcggggga agggggacat
     9001 gattaataga gtcgagcgtg cgttgtggca tccgcgggac tcgtgaatag cggaatatag
     9061 agttctggac aggggtagag taaagcgaag gggtgtcggc cagcggagcc ggggatatcg
     9121 cgcgagccgg taaaagtctg cctgcgagga gcccggccgg gggggttccg gcgggcggac
     9181 agcggaaagg ggaaagagat ggcagaggga ccatacctca taggcattga cggcggcact
     9241 ggaggcgtga gggccggcat cttcgaccgc gagggcacgg cgctgatctt caacggcgtc
     9301 gagcacggga cgaagttccc gcgttcggga tgggcggagc aggacccgga cgactggtgg
     9361 tcgtgcctcg tgcaggcggt gcggggcgcg atggaagaga gcggtgtcgc gcccgaggag
     9421 atcgccggta tctccacgga cgctacgacc tgcacggtcg tagccatgga cgcccagaac
     9481 cgcgtgctga ggccggcgct cctgtggatg gacgtgcgcg cctccgacca ggcccgacgg
     9541 atccaggaga ccggcgaccc ggcgctgaag tacaacggct acggggcggt ctccgccgag
     9601 tggatgccct caaaggcgct gtggatcaag gagaacgaac cggagaccta caacgaggcg
     9661 gcgcgcatct gcgactacgc ggactggatg accttccgcc tgaccggcga gtggaccgcc
     9721 tcggtcaaca ccgccgcctt ccgcgcatac tacgaccgcg acgcgggcgg ctggccggag
     9781 agcctgtact cggccatcgg gctggaagac gtgctggaga aattcccgcc aaaggtgctc
     9841 gacatgggca cggccgtcgg cgggctcacg aaggaagcag cggatgagtt gggcctgccg
     9901 gaaggcatcc cgatagccca gggcggcggg gacgcttttg tgggcacgct gggcctcggg
     9961 gttacggagc cgggtaagat cgcgttcatc accggctctt ctcacgtcat cgtggggcag
    10021 gccgccgagc cccactacgg ccggggcttc ttcggctctt ataccgacgg catagtgccg
    10081 ggccagtaca gcgtcgaggg cgggcaggtc tcgaccggct ccatcgtcgc ctggttcaag
    10141 aacagcttcg cgggcgacgc ggcggcggag gccgagaggc gcggcgtgga cccctacttg
    10201 gtgctaaacg agatggccgc ggaggtgccc atcggctcca acggcctgat cgtggtggac
    10261 tacttccagg gcaaccggac cccgtatacc gactcgctgg cgcgaggcat gatctggggc
    10321 ctctcgctcg gtcacggcac gggccacatc ttccgcgcga tcctcgaagg catctgctac
    10381 gggacggagc atatcttccg caccatgcgt cagaacggct tccagccggg agaggccgtc
    10441 gccgctggag gcccgaccaa gagcgagctt tggatgcaga tgcacgccga cgtctccaac
    10501 gtgccgatct ccttcacccg cgagagcgaa ggcccggtcc tgggctgcgc catgctggcc
    10561 tcggtcgggg ctgagatcta ccccgatatc cagaccgcgg cggacaacat ggtccacacc
    10621 gagcggacca tagagccgaa ccccgaggcg cacgaggcgt accagttcta cgtggaccgc
    10681 tacgtcgaga cgtatccgcg gatgcgggag ctgatgcaca aggtggtggg gcacgaggcc
    10741 gcccacgggc acatgcaata ggccgggata cggatcgatc cggagcatcc attagcgcag
    10801 gcgtactggc gttatccggt tacgggtctt atccgggcag cgccgcgagc accacgaaac
    10861 aagacagaga ggagcgcgaa caatggcaga tcaggccggg gccggatcca ccggcaccct
    10921 actgctcccg gaccggctct acaaaggcta tgtcttcgac ctggacggga ccatctacct
    10981 gggcgacgaa ctgctgcccg gagcgaagcg cctgatcctc gagctgcgcg agcggggcaa
    11041 gaaggtggtc ttcctctcca acaaccccac caaagacccg gagatgtacg ccgagaagct
    11101 caccaaactc ggccttccca ccccgcaaga agagaccgta aacaccgtcg tcaccatgac
    11161 ccagtggctc ctccagaacc acccggacgc gacggtcttc cccatagccg aggagcccct
    11221 gaaacgttcg ctgcggaacg ccggcatcaa gatgtccgag gcccccgagg agatagacat
    11281 agtcatcgcc tcctacgacc ggacattcga gtaccggaag ctccagatcg ccttcgacgc
    11341 catctggttc cacaagcggg cgcgcctcgt caccaccaac ccggacaaat actgcccgtt
    11401 ccccggaggc cggggcgagc ccgacgcggc gtccatcgtc gcggccatag aggcgtgtac
    11461 cggagcgaag tgcgaggtca acaccggtaa gcccgacccg atcatgcttg agacgatcat
    11521 gggcatgatc gggatggacg cctccgagtg cgtgatgacc ggggaccggc tctacacgga
    11581 gatccggatg gccctcgacg ccgggatgcc ctcggccatc gtcctcaccg gcgagacgac
    11641 cacggagatg ctcgccgcgg agagcgagga gaacaagccg gactacgtgc tcgacaggat
    11701 agaccgcctc atcccgcagg acatctggga tgagcttggc tggaccgaag aagacgagta
    11761 acaggaggag atgagttcaa gcatgagcgc agagcggata aacgcggcgt tgcgtgacgc
    11821 gaccgacacc cagacggtcg agatcgggcc cggagccctc gccacggtcg ccgaagtctt
    11881 cgagcagagc ttcggcgacc ggacggccat cgtcgtaacg gacgagaata tatgggcggt
    11941 ggccggagag gaggtgcagc ggaacctcga agcggccggc cgcgacaccg ccgagccata
    12001 catctttccc ggcaaaccga ccctgtacgc agggtacgac aacatacaga tactagtaga
    12061 ggccctgcgc ccccaggatg ctatcccggt cgccgtgggc tccgggacgc tcaacgacat
    12121 cgtcaagcgg gcggcatacg agctggagcg gccgtacatg aacgtcgcca ccgccgcctc
    12181 gatggacggc tacacggcgt tcggcgcctc catcgagaaa gacaacaaga agcagacgct
    12241 gacctgcccg gcgccccgcg cggtggtagg cgacgtggag gtcctggtaa acgcgcccga
    12301 gaggatgacc gcatcgggtt acgccgacct gctgggcaag gtaacggcag gcgccgactg
    12361 gctggttgcc gacgcgctcg gggtggagcc catctcgcag aaggtctggt ccctggtgca
    12421 ggactccctg cgggactgga ccgagagacc ggcggagttg cgcggggggg atcaggccgc
    12481 gatggacggg cttatagaag gtttgatcct gtccggcctc gccatgcagt ctcaccagtc
    12541 ctcccgtccc gcctccggcg ccgagcacca gttcagccac ctgtgggaag gagagggact
    12601 cggccgcgac ctggacccgc cgctctccca cggattcaag gtgggggtgg gctccatcgc
    12661 catcgcagcc ctcttcgagc gggtactgga gcgcgacctg agcaacatag acgtggacgc
    12721 gctctgcgct agctggcccg cctgggagga catggaaaag agcgtgcgcg aggcatactc
    12781 gggctcgaag ctggagcagg ccgccgtcaa cgagacccag gccaagtaca tagacgccga
    12841 cacgctccgg gaacggctgg agaggctgcg cgaggtctgg ccggagctgc gcgggaaggt
    12901 ggaagagcag ctaatgcccg ccggcgagtt gcgggagcag ttgcgggcgg cgggatgccc
    12961 gacgagcccc gaggagatcg gactaagccg ggaagagttc aaggcgacgt acttcagggc
    13021 gcagatgctc cgcaggcgct acaccatcct cgacctggca ctggagaccg gcatcctgga
    13081 cgagtgcgtg gaggagctgt tcgcgccgga tggtttctgg gcgtccgaca ccgcctcgca
    13141 agagatccgc taaggaggtc tgaagtgacg aagaagatcg gcataggcgc cgacgacgcg
    13201 gccctggaac tgaaggccac gctcgtcgag cacctgaaag agaagggcta cgaggtcgag
    13261 gactacagtc cccgctccgc ggaagaggcc caggccgccg gggttgacta cccggacgtg
    13321 gccgtctcgg tcgcggaggc ggtcgccagg ggggatcacg accgggcgat actctgctgc
    13381 gggaccggga tcggggtgaa tatcaccgcg aacaaggtgc cgggggtgcg ggcggcccag
    13441 tgccacgacg tcttctccgc cgagcgggcc aggaagtcca acaacgccca gatcctgacg
    13501 atggggcagc gcgtgatcgg gccggagctc gccaagacga tcacggacgc ctggctcgaa
    13561 tccgagttcg ggggcggcgg ctcgacgcgc aaggtcgaga agatggaaga ggtggaccgc
    13621 cgctaccacg ccgccgggta aagggaggtc ggggagccac gcacggagca ggtcgtccgg
    13681 cgagagccgg cgtacacgcg acggcccggc tccccaaccg ggcaaaagga gatatcttgg
    13741 aagtcaagac cgggaacgtc gagcaactct ctgtaaacac tattcggact ctgtcgatgg
    13801 acgcggtaca ggcggcgaac tccgggcatc cggggacgcc gatggccctg gcgccggtgg
    13861 tc
//
LOCUS       DASSMB010000230         8452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_448529, whole genome shotgun sequence.
ACCESSION   DASSMB010000230 DASSMB010000000
VERSION     DASSMB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448529"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /gene="tilS"
                     /locus_tag="VFJ72_13510"
     CDS             <1..792
                     /gene="tilS"
                     /locus_tag="VFJ72_13510"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7480520.1"
                     /translation="NLTGKYARNRVRLEVLPVLEELYPGAGANIARAADLVRQDLDAL
                     ESMAARLVSRRNREMVVPLGALMEAPPALRRYAVRRAYAALAPEAPPLAQALVENVLR
                     LAEGENGTRMLDLPGGVVVAVRFAEEVAFYRRPDVVDTEHQLRAGEASFGGWEISVRE
                     VAELDARDAARAEVAYLDEALGPYRVRYAREGDAIRPLGLGGTKKVLRAMMDRKVPRD
                     LRRRMPVVVDRRDRVAWIFLGELDEAFAVTPATEKVLRMEVLRTP"
     gene            789..1346
                     /gene="hpt"
                     /locus_tag="VFJ72_13515"
     CDS             789..1346
                     /gene="hpt"
                     /locus_tag="VFJ72_13515"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET7480521.1"
                     /translation="MKMSSMMPDVQEVLIPSDEIQAKLRELGERITEDYRGEKPLLVG
                     VLRGAILVMSDLMRNIDLQCEIDFMDISSYGSGTSSSGVVRILKDLEEDITDRRVLIV
                     EDIIDTGLTLSYLLRSLLARKPASLEVCALLSKPSRRRVELDVKYLGFEVPDEFVVGY
                     GLDYAGAYRNLPDICILKPEVFGGA"
     gene            1509..3344
                     /gene="ftsH"
                     /locus_tag="VFJ72_13520"
     CDS             1509..3344
                     /gene="ftsH"
                     /locus_tag="VFJ72_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7480522.1"
                     /translation="MRVLSAGSPDVKQLNSQQFQKALDQKSFVTDVPEGKDGQLTVKD
                     QDQTVTGLLKNGKGGEKFEYAYPQQYDIASAFNKAKIPFTTDPQNTGFWLSVLATLAP
                     IFLIVLLFLFIMSSMQGGGNRVMSFGKSRARRMTKDSPKVTFADVAGANEAVQELTEI
                     KEFLEAPQKFQKLGARIPKGALLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVEMF
                     VGVGASRVRDLFEQAKQNSPCIIFMDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMD
                     GFDSKSGIIMIAATNRPDILDPALLRPGRFDRQIVVDRPDLPGREKILRVHTRGKPLG
                     DDISIETLARSTPGFTGADLANLVNEAALLAARHDKDQIEQSEMEEAIDRVIAGPERK
                     TRLISKKEKKITAYHEAGHAVVGALLPDADPVHKITIIPRGQALGVTMSLPTEDRFMM
                     SKSELMAQLAMMLGGRAAERVTFDEITTGASNDLERVTQTAKQMVTRYGMSEKLGPMA
                     LGHANQQVFMGRDFGAQPDYSDEIAFQIDKEIRRIVDESYDTAEDLLIRNRTLVEQLA
                     KDLIEYETVDAKHLLRLVEEYAVDKVSPNGAVPDADGHQHNRYLD"
     gene            3345..4217
                     /gene="folP"
                     /locus_tag="VFJ72_13525"
     CDS             3345..4217
                     /gene="folP"
                     /locus_tag="VFJ72_13525"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET7480523.1"
                     /translation="MRRAPAAEKTIRAGDRELGPGPVIFGILNITPDSFSDGGEFFDR
                     DAAVAHATGMLDEGAHVIDVGGESTRPGSDPVPGEDEVRRVVPVIRDILAERPEALIS
                     IDTYRASTAEAALEAGVRIVNDVTALRGDPRMADLVAEAGCPVVLMHMLGEPKTMQRD
                     PRYGDVVREVCDFLAARAEFAVAAGVDPANLILDPGIGFGKTLEHNLTLLNSIETIVE
                     IGLPVLVGASRKRFLGKITGAETASKRVAATVATTVLAYERGAALFRVHDVRENADAL
                     AVAEAVRKAQSSRA"
     gene            4214..4702
                     /gene="folK"
                     /locus_tag="VFJ72_13530"
     CDS             4214..4702
                     /gene="folK"
                     /locus_tag="VFJ72_13530"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET7480524.1"
                     /translation="MSRAFLSFGSNLGDRLYYLRSAVSALRFAPSVEVLNVSRVYETE
                     PVEVEEAQPEYLNCVVEAECGLPAVELLRFCQGVEAGLGRVRKGEKAPRTVDIDVLLF
                     GEEVVEGRELALPHRGILRAFNLRGLADLDPSVYIPGRGMVGDLLAEADLSGVREYGE
                     GV"
     gene            4703..5476
                     /locus_tag="VFJ72_13535"
     CDS             4703..5476
                     /locus_tag="VFJ72_13535"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET7480525.1"
                     /translation="MLLAVDIGNTQTVLGLFEGEELRGHWRLATESHRTADELAVVFA
                     GLLDLNGQGMRDVSAMIVSSVVPALSRSYSDLADDVFGVPFYRVTMDMKTGLDNRYDD
                     PSAVGADRIVNAVAAGHYYGFPAIIVDIGTATTVEAVDGDGCYLGGAIMTGLDVALEA
                     LVSRTAKLPSVDLEGDPPNAIATNTPDSIRSGFIYGYAGAIDALIRRSREELGAENVR
                     TIVTGGPAGAIARHCQEIQEYDPDLTLKGLRVLYEMNVS"
     gene            5593..6078
                     /gene="greA"
                     /locus_tag="VFJ72_13540"
     CDS             5593..6078
                     /gene="greA"
                     /locus_tag="VFJ72_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET7480526.1"
                     /translation="MTDKNQELVTREGLEKLQEELTHLTEVRRKEVADRIRQAREFGD
                     ISENSEYDDAKTEQGLLERRISEVQRRVRNAKVVDPSETEQGSVNVGTVVTLRRVGKD
                     EERTFQIVGANESDPTSGKLSHASPVGRAVLKRRVGEKVTVATPRGSTEYEIINVEAA
                     S"
     gene            6130..7593
                     /gene="lysS"
                     /locus_tag="VFJ72_13545"
     CDS             6130..7593
                     /gene="lysS"
                     /locus_tag="VFJ72_13545"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF014031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET7480527.1"
                     /translation="MSETRLPIPDWAVKVADTLGEGPHVVVTGISPSGNIHNLREVLV
                     AEAVANALKARGEVVRFIFHADTIDPLRKISPGIPESFEKYMGHSLSHIPDPEGCHAS
                     YADHFLVPFEEALNEMEMEVDVLRSHELYESGVYAEVTREAIEHTGELRRILQEVSAR
                     QLPESWSPFLPRAASGDLRDNRVVEHRPEENVVAFVDREGNEGVADYSKGEGKLGWRT
                     ELAARWKALGVTFEPFGKDHTSRGGSTDTADRMAREVFDYPAPGRYEYEWISIKGQGP
                     MSSSKGIVLLPKDLLRIMPPDALRRMMLGRDPARALDLDLGEGFPRFMDEYRGETEEN
                     RVPFTHLVTVAQTVGKDTGAAVQMLRRGGYEEAAKDPDKLARDLYYARNWAEEWAPES
                     LRLRILEPGESGEAAASLDEEQRDYLAAVARKLEPGMEGEAVQDLLYKTAVERGIKPK
                     RAFAAVYTVLIGKKSGPKAGPFVAGLPFAEVRKRFAV"
     gene            7895..>8452
                     /locus_tag="VFJ72_13550"
     CDS             7895..>8452
                     /locus_tag="VFJ72_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7480528.1"
                     /translation="MFERFTERARKVVVLAQEEARHFNHNYIGTEHLLLGLLREDEGV
                     AARALSSLNVTLDEVREQVESIVGYGEEGTGGQAPFTPRSKKVLELALREALQLGHNY
                     IGTEHILLGLVRESEGVAARVLSNLDVDPDRIRRQVVQQLGGGRSQRGGRGGEAGGRG
                     GVEAKRPKTRQLDQYGRNLTAYAEED"
BASE COUNT         1611 a         2543 c         2894 g         1404 t
ORIGIN      
        1 aacctcaccg gcaagtacgc ccgcaaccgc gtgcgcctgg aggtcttgcc cgtccttgag
       61 gaactgtatc ccggcgccgg tgccaacatc gcgcgcgccg cggacctggt gcgccaggac
      121 ctggacgctc tggagagtat ggcggcgcgg ctggtgagcc gccggaacag ggagatggtc
      181 gtcccgctcg gagcgcttat ggaggcgccg ccggcactgc ggcggtacgc ggtccgccgg
      241 gcctacgcgg ctctggcgcc ggaagcgccg ccgctggcgc aggcgctcgt cgaaaacgtg
      301 ttgcggctcg cggagggaga aaatggcacg cggatgctgg atctccccgg cggcgtggtc
      361 gtagctgtcc gctttgcgga ggaggtggcg ttctaccgca ggccggatgt ggtggatacg
      421 gagcaccagc ttcgggcggg cgaggcttct ttcgggggat gggagatctc ggtccgcgag
      481 gtcgcggagc tggacgcgcg ggatgcggcg cgcgccgagg tcgcgtatct tgatgaagcc
      541 ctggggcctt atcgggtgcg gtacgcgcgg gagggcgatg cgatccgtcc tctcgggctc
      601 ggtgggacga agaaggtatt gcgggctatg atggaccgga aggtgccgcg ggatctccgg
      661 cgtcggatgc cggtggtggt cgacaggcgg gatagagtcg cctggatctt cctcggcgag
      721 ctagacgagg cgttcgcggt aacgccggcg acggagaagg tgctacgtat ggaggtgctc
      781 agaacgccat gaagatgtcc agcatgatgc ccgacgttca ggaagtgctc ataccatcgg
      841 atgagatcca ggcaaagctc cgcgagctgg gagagcgcat aaccgaggac taccgcggcg
      901 agaaaccgct gctcgtcggc gtcctgcgtg gggcgatact cgtaatgagc gacctcatgc
      961 gaaacataga cttgcagtgc gagatcgact tcatggacat cagttcctac ggctcgggca
     1021 cctcttcgag cggcgtcgtc cgcatcctca aggacctgga agaagacata accgaccgcc
     1081 gcgtcctcat cgtcgaggac ataatagaca ccgggctcac gctctcctac ctgctgcgct
     1141 cgctgctcgc ccgcaagccc gcctccctgg aggtctgcgc cctgctctcg aaaccgtccc
     1201 gccgtcgggt ggagctagac gtgaagtacc tgggcttcga ggtccccgac gagttcgtcg
     1261 tgggctacgg cctcgattac gccggggcct accgcaacct cccggacatc tgcatcctca
     1321 agcccgaggt cttcggcggc gcgtaagctg gagcggtaag acatacactc ttcgattccg
     1381 ggcgcaggta gtataatccg cgtgttcctc aatctaccct acgagaggca aaggcggttt
     1441 tgggcagaat cttaagaagt ggcggactgc tttactttat tatcctgatg gtattcgcgg
     1501 tggtcctggt gagggttctc tcggctggca gcccggatgt caagcaactg aactcccagc
     1561 agttccagaa ggcgctggat cagaagtcgt tcgtgaccga cgtacccgag ggcaaggacg
     1621 gccaactcac ggtcaaggac caggatcaga ccgtaaccgg cctgctcaag aacggcaagg
     1681 gcggcgagaa gttcgagtac gcctatccgc agcagtacga tatagcctcc gctttcaaca
     1741 aggccaagat acctttcacc accgacccgc agaacaccgg gttctggctc agcgtgctgg
     1801 ctacgctcgc gccgatcttc cttatagtcc tgctcttcct cttcataatg agctcgatgc
     1861 agggcggcgg caaccgcgtt atgagcttcg gcaagagccg cgcccggcgc atgacgaagg
     1921 actccccgaa ggtgacgttc gcggacgtgg ccggagcgaa cgaggcggtc caggagctca
     1981 cggagatcaa ggagttcctc gaagccccgc agaagttcca gaagctcggc gctcgtatac
     2041 cgaagggcgc cctgctcgtc gggcctcccg gaacgggcaa gacgctcctg gcgcgggccg
     2101 tggccgggga ggccggggtc ccgttcttct ccatctccgg ctcggacttc gtggagatgt
     2161 tcgtcggcgt cggcgcgagc cgggtgaggg acctgttcga gcaggccaag cagaactcac
     2221 cgtgcatcat cttcatggat gagatcgacg ctgtcggcag gcagcgcggg gccggcatgg
     2281 gcggcggcca tgacgagcgc gagcagacct tgaaccagct cctcgtcgag atggacgggt
     2341 tcgactccaa gtccggcatc atcatgatcg ccgcgaccaa ccgcccggac atcctcgacc
     2401 cggccttgtt gcgtccgggg cgcttcgacc ggcagatagt cgtggatcga cccgaccttc
     2461 cgggacggga gaagatcctg cgggtccaca cccgcggcaa gccgctcggc gacgacatct
     2521 caatcgagac gctagcccgc tcgacgcccg gcttcaccgg cgcggacctc gccaacctgg
     2581 tcaacgaggc ggccttgctc gccgccaggc acgacaagga ccagatagag cagtccgaga
     2641 tggaagaggc catagaccgg gtcatcgccg gtcccgagcg caagacgcgc ctcatctcca
     2701 agaaggaaaa gaagatcacg gcgtaccacg aggcgggaca cgccgtcgtt ggggcgctgt
     2761 tgccggatgc ggacccggta cacaagatca cgatcatccc tcgcggccag gcgctcggcg
     2821 tgacgatgag cctgccgacg gaggaccgct tcatgatgag caagagcgag cttatggctc
     2881 agctcgccat gatgctcggc ggccgggccg cggagcgggt tacgttcgac gagatcacga
     2941 ccggcgcctc gaacgatctg gagcgcgtta cgcagaccgc caagcagatg gtcacgcgct
     3001 acgggatgag cgagaagctg gggccgatgg ctctggggca tgccaatcag caggtcttca
     3061 tgggccggga cttcggcgcg cagccggact actccgacga gatcgccttc cagatcgaca
     3121 aggagataag gcgcatagtg gacgagtcct atgataccgc cgaggacctc ctgatccgca
     3181 accgtactct ggtcgagcag ttggcgaagg acctgatcga gtacgagacc gtggacgcga
     3241 aacacctttt gcgcctggtc gaggagtacg ccgtggacaa ggtatcgccc aacggggccg
     3301 tccctgacgc tgacggccac cagcacaacc gctacctgga ctagatgcgc cgcgcaccgg
     3361 cggcagagaa aacgatcagg gccggagacc gtgaactcgg tcccggcccc gtcattttcg
     3421 gcatcctcaa cataaccccc gactccttct ccgacggcgg agagttcttc gaccgcgatg
     3481 cagccgtcgc ccacgcgaca gggatgctgg acgagggcgc ccatgtcata gacgtgggcg
     3541 gtgagtccac ccggcccggc tcggacccgg tgccgggaga ggatgaggtc cggcgcgtcg
     3601 tccctgtcat ccgggacata ctcgcggagc gcccggaagc actcatctcc atagatacct
     3661 accgtgcctc gactgccgaa gccgcgctcg aagccggggt ccgcatcgtc aatgacgtga
     3721 ccgcgctgcg cggcgacccg cggatggccg acctagtcgc cgaagccggt tgtccggtgg
     3781 tcctgatgca catgctcggc gaaccgaaga ccatgcagcg agacccccgg tacggcgatg
     3841 tggtgcgcga ggtgtgcgac ttcctggcgg cgcgggcgga gtttgccgtt gcggcgggtg
     3901 tggaccccgc gaacctcatc ctggaccccg gcatcggctt cgggaagact ctggagcaca
     3961 acctgacgct cctcaatagt atcgagacca tcgtggagat cggcttgccg gtgctcgtcg
     4021 gggcctcgcg caagaggttc ctcggaaaga tcacgggcgc agagacagcc agcaagcgcg
     4081 tcgccgccac ggtagctacc acggttctgg cctacgagcg cggcgcggcc ctcttccgtg
     4141 tgcatgacgt gcgggagaac gcggacgcgc tcgccgtcgc cgaggccgtt cgtaaggcgc
     4201 agtcgtctcg cgcttgagcc gggcgttcct cagtttcggt agcaacctcg gagaccgtct
     4261 ctattacctg cggtcggcgg tcagcgcgct ccggtttgcg ccctcggtcg aggtgctgaa
     4321 cgtgtctcgg gtctatgaga cggagccggt cgaggtcgag gaggcgcagc cggagtacct
     4381 gaactgcgtg gtggaggcgg agtgcggcct gccggcggtc gagttgctgc ggttctgcca
     4441 gggagtcgag gcgggactgg gacgggtgcg gaaaggtgag aaagcgccgc ggacggtgga
     4501 tatagacgta ttgctattcg gggaggaagt cgtcgagggg cgggagttgg cgctgccgca
     4561 ccgggggatc ctgcgggcgt tcaacctgcg ggggctcgcg gatctcgacc ccagcgtata
     4621 tattccgggg cgcggtatgg tcggagatct tctggcggag gcggacttga gcggggtccg
     4681 ggagtatggg gagggcgtct aggtgctgct ggcggtggat ataggtaaca cgcagacggt
     4741 actcggtctc ttcgagggcg aggagcttag gggacactgg cggctagcca cggagtcgca
     4801 caggaccgcc gacgaactgg cggtggtctt cgcggggctg ctggacctga acgggcaggg
     4861 gatgagggac gtgagcgcca tgatcgtctc tagtgtcgtg ccagcgctct cgcgatcgta
     4921 cagcgacctg gcggatgatg tgttcggcgt gcccttctac cgggtgacga tggacatgaa
     4981 gaccggcctc gacaaccgct acgacgatcc gagcgcggtg ggggccgaca ggatcgtgaa
     5041 cgccgtcgcc gcgggacact attacgggtt cccggcgatc atagtggaca tcgggacggc
     5101 gacgaccgtg gaggccgtgg atggagacgg ttgctatctg gggggcgcca taatgaccgg
     5161 cctcgatgtc gcgctggagg cgctagtctc ccgaactgcc aagctcccga gcgtggatct
     5221 tgaaggcgat ccgcccaacg ctatagcaac taatacgccg gactccatcc gcagcggatt
     5281 tatatacggt tacgctggcg ccatagacgc tctcatccgc cgctcgcgcg aggagcttgg
     5341 ggcggagaac gtccgcacca tcgtcaccgg tgggcccgcg ggagccatcg cccgccactg
     5401 tcaggagata caggagtacg acccggacct cacgctcaag gggctgagag tactgtacga
     5461 gatgaacgtc tcctagccgc gaaccatcag agtcgcgcac tcggctgccg ctcttgctga
     5521 aagccaatcg ccgggtgctg atgtatctcc ccgttgacac tccataagcc cgctcgtata
     5581 ctctcacggc ttatgacgga caagaaccag gagctcgtga cccgtgaagg tctggagaag
     5641 ctccaagagg aacttacaca tctcaccgag gtgcgccgca aggaggtcgc ggaccgcatc
     5701 cggcaggccc gcgagttcgg ggacatctcc gagaactccg agtacgacga cgccaagacc
     5761 gagcaggggc ttctggagcg caggatcagc gaggtccagc gccgggtccg caacgcgaag
     5821 gtagttgacc cttcggagac ggaacagggc tccgtcaacg tcgggaccgt ggtgaccctc
     5881 cggcgggtcg gcaaggacga ggagcggacc ttccagatcg tcggcgccaa cgagtccgac
     5941 ccgacgagcg gcaagctctc gcacgcctct ccggtcggtc gcgcggtatt gaagcgccgc
     6001 gtgggagaga aggtgacggt cgccacgccc cgcggatcga cggagtacga gatcatcaac
     6061 gtcgaggccg cgagttaact tctccaccgc gctccgcccc ttatcaccct accaaatgtg
     6121 gagcaccgaa tgagcgagac cagattaccc atacccgatt gggccgttaa ggtcgcggat
     6181 accctcggag aaggaccgca cgtcgtcgtc accgggatca gtccttccgg gaatatccac
     6241 aatctgcgcg aggtgctcgt cgccgaggcc gtggcgaacg ccctgaaggc gcgcggcgag
     6301 gtggtccgct ttatctttca cgccgatacg atagacccgc tgcggaagat ctctccaggc
     6361 atccccgaga gtttcgagaa atatatggga cacagtctct ctcatatccc cgaccccgaa
     6421 ggctgccacg cttcctacgc ggatcacttc ctcgtgcctt tcgaggaggc attgaacgag
     6481 atggagatgg aagtagatgt cttgcgttcg cacgagctct acgagagcgg cgtctacgcg
     6541 gaggttacgc gggaggccat agaacatacc ggggagctgc gccgtatctt gcaggaggtc
     6601 agtgcgcggc aactgccgga gtcctggtcg ccgttcctgc cgcgggcggc gtccggggat
     6661 ctcagggaca accgcgtagt cgagcaccgc ccggaggaga acgtggtcgc cttcgtggac
     6721 cgggagggga acgagggcgt cgcggactac tcgaagggtg agggcaagct cggatggcgg
     6781 acggagctcg cggcgcggtg gaaggcgctc ggggtgacgt tcgagccgtt cggcaaggac
     6841 cataccagcc gcggcggctc cacggatacg gcggacagga tggctcgcga ggtcttcgac
     6901 tacccggcgc cggggaggta cgagtacgag tggatctcca taaagggcca ggggcccatg
     6961 agcagttcga agggcatagt ccttctgccg aaagacctgc tcaggatcat gccgcctgac
     7021 gccctgcgac ggatgatgct cggccgcgac ccggcccgcg ccctggacct ggacctcggc
     7081 gaaggtttcc cgcgttttat ggacgagtat cggggagaga ccgaggagaa ccgtgttccc
     7141 tttactcatc tggtaacggt cgcccagacc gtcggaaagg acaccggcgc tgcggtccag
     7201 atgttgcgcc ggggcggata cgaggaggct gcgaaagatc ccgataagct ggctcgcgat
     7261 ctctactacg ctcgtaactg ggccgaggag tgggcgccgg agtcgttgcg gctcaggata
     7321 ctggaacccg gagagtccgg tgaggctgct gcgagcctgg atgaggagca gcgcgattac
     7381 ctcgccgccg tggcgcggaa gctggagcca gggatggaag gcgaagcggt gcaggacctc
     7441 ctgtacaaga ctgcggtgga gcgggggatc aaaccgaaac gcgccttcgc ggccgtctac
     7501 accgtcctga tcggtaagaa gagcggcccg aaggccggcc cgttcgtcgc cggactgccg
     7561 ttcgcggagg tgcgaaaaag gttcgccgtg tgatggcgga caccgcgccg cggcatgccg
     7621 gactgtaatt tagaaagcgc aagagagagg gtttcgcggt gacgctacac aacctatagc
     7681 gctgagagcg taaagcgctg gagagggtca taatgaggac gaaacctttg tatactcata
     7741 agcagccgag gctcgaccag agaggtaccg agatttcaga ggaaagattt ttgacgatgt
     7801 ataatctatt tgtttctatc agtcgtatac catttacaat agccactgcc cccgaaacgg
     7861 tgaaggaccc tcaaaataga ggaggctccc gctaatgttc gagagattca cggagcgagc
     7921 ccgcaaggtc gtggtcctgg ctcaagagga agcccgccac ttcaaccata actacatcgg
     7981 cacggagcac ctgctcctcg gcctcctgcg cgaggacgag ggtgtggcgg ctcgcgccct
     8041 gtcttccctg aacgtcaccc tggacgaggt gcgcgagcag gtcgagagca tcgtcggtta
     8101 cggcgaggag gggacgggcg gtcaggcgcc gttcacacca cgctccaaga aggtgctgga
     8161 gttggcgctc cgcgaggcgc tgcagctcgg gcacaactac atcggcaccg agcacatcct
     8221 gctcgggctg gtcagagagt ccgagggtgt tgcggcaagg gtcctcagca acctggacgt
     8281 tgacccggat cgcatacgtc gccaggtcgt acagcagctc ggcggcggcc ggtcgcagcg
     8341 ggggggccgg ggtggcgagg ccggtggacg cggcggagtg gaggccaagc ggcccaagac
     8401 ccgccagcta gaccagtacg gcaggaacct cacggcgtac gccgaggaag ac
//
LOCUS       DASSMB010000231         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_68098, whole genome shotgun sequence.
ACCESSION   DASSMB010000231 DASSMB010000000
VERSION     DASSMB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68098"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFJ72_13555"
     CDS             complement(<1..569)
                     /locus_tag="VFJ72_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480529.1"
                     /translation="MSGTLLLLTSFLASGVEAVEALTIVLAVGITRGWRTSLAGMLLA
                     LVALTVVVAALGPALVLYVPLDALRVLVGALLLVFGLQWLRKAVLRASGLKALRDEDR
                     AFQDDVARLSGGTKQRTIDRTGFVLSFKGVFLEGLEVAFIVITFGASSGRLGLSATGA
                     LIAVGLVALVGVAVHRPLSRVPENVIKFAV"
     gene            complement(648..1298)
                     /locus_tag="VFJ72_13560"
     CDS             complement(648..1298)
                     /locus_tag="VFJ72_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7480530.1"
                     /translation="MKVLVAGAHGQVARHLVRMLAEDGHEVRGLVRKEEQLPDVESDG
                     AEPVLCDLEKEEVEGGVGEAVEGCDAIVFAAGAGPGSGNPRKETMDYGGAAKLIEAAE
                     KHGVPRYLILSSTGADDPESRDEAMRPYLRAKARADERLEQSGLDYTIIRPGRLTDDP
                     GTGRIDAAESLGRYAEIPREDVARVFAAALEAPNTYGKKFEILAGETPIEEAVEKI"
     gene            complement(1436..1990)
                     /locus_tag="VFJ72_13565"
     CDS             complement(1436..1990)
                     /locus_tag="VFJ72_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480531.1"
                     /translation="MAKLTTAAWIAHNLGIGASFGGTLFGKLALNPKLDVLSSEPDRG
                     KLLNAAWNRYNVVNALTFGTAALTWFVGRAGISGESIDEDARNLVLTKDALFIAAALT
                     GLASIISGLSLARQAPDGAVPIESGTTPSARTPEEASKLLRRVNVLGNVNLALVGAIG
                     AITTILSQKAGQSAKWSAVSRFLP"
     gene            complement(2084..3430)
                     /locus_tag="VFJ72_13570"
     CDS             complement(2084..3430)
                     /locus_tag="VFJ72_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7480532.1"
                     /translation="MSMDARGGAVRGTRRFRRGEFLRIGGAGLAGAALLGSGALAGCG
                     GGEEGGSGTVTYAEGPDDTGTLKKLLDGFNKKYKGKYTARHREMPADTQGYFDKLRTE
                     LQAGNSDIDVIAGDVIWPAQLASNGWLMDLSDRFTKGLQDKFIKGNVESNVYKGKIYG
                     VPFVTGAGLIYYRKDLLEKSGFTSAPETWDELKQMALKVKQDSGTRFGFVFEGANYEG
                     GVCDGLEYIWTHGGDVLKGDRVVIDSPEAAAGLATYHSMVSDGVTTEAMATYKEEEAQ
                     GAFLRGDAVFMRNWSYVYALTSDPAQSKIKPEQVGLSTLPVARKGMPLSSALGATNLF
                     INSTSKNADGAWALIKYFTAPEQQKMRAIEGARLPVTKVSYEDQELLKKVPVMKLGKE
                     ALKTARPRPVSPYYSDMSLKMAEEFFNVLKGDTAPEQAVGRLQGELQSIIKQGKNA"
     gene            3519..>3779
                     /locus_tag="VFJ72_13575"
     CDS             3519..>3779
                     /locus_tag="VFJ72_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480533.1"
                     /translation="MHPPTPPTERPHRNHGLFSDHFLNATLPGRPDWRALADEARPAM
                     EDISRLLDSYTPSTNEAQVEEELVRPVLRLLGHERSYEVQPAL"
BASE COUNT          614 a         1308 c         1173 g          684 t
ORIGIN      
        1 acggcgaact tgatgacgtt ctcggggacg cggctcagag gccggtggac ggccaccccc
       61 acgagcgcga ccagtcccac cgcgatgagg gcgccggtcg cgctcaggcc cagtcgtccg
      121 gagctggcgc cgaacgtgat cacgatgaac gccacctcca gcccctccag gaagactccc
      181 ttgaacgaca gcacgaagcc ggtccggtct atggtcctct gcttcgtacc tcccgagagc
      241 cgcgccacat cgtcctgaaa ggcccggtcc tcatcgcgga gcgccttcag gccggaggcg
      301 cgcaggaccg ccttccgcag ccattgcagg ccgaagacga gcaagagagc gccgaccagg
      361 acccgcagcg cgtccagcgg cacgtagagc acgagcgccg gtccgagcgc cgccaccacc
      421 acggttagcg ccactagcgc caggagcatt cccgcaagag acgtgcgcca gccgcgcgtg
      481 atcccaacgg ccagcacgat ggtgagcgcc tctaccgcct cgactcccga ggccagaaaa
      541 gatgtgagaa gcagcagagt cccgctcaag ggttcgacct cctggcaagc gcgctacacg
      601 gaacgaagag atgatacccc ctcccggcgg cgaagcgcgc gtggacgtta gatcttctcg
      661 accgcctcct caataggagt ctccccggcc agtatctcga acttcttgcc gtaggtgttc
      721 ggcgcttcga gagcggcggc gaacacgcgg gccacgtcct cgcgcgggat ctccgcgtac
      781 cgccccagcg actccgccgc gtctatccgg cccgtgccgg gatcgtccgt gagccttccg
      841 ggccggatga tggtgtagtc cagaccgctc tgttccagcc gctcgtcggc acgggccttt
      901 gcccggaggt agggacgcat cgcctcgtcg cggctctccg ggtcgtcggc gcccgtggaa
      961 ctgaggatga ggtagcgcgg cacgccgtgc ttctcggcgg cttcgatgag cttcgcggcc
     1021 ccgccgtagt ccatcgtctc cttgcgggga ttcccgctac ccggtccggc cccggcggcg
     1081 aagacgatcg cgtcgcaacc ctcgacggct tctccgacgc ctccctctac ctcttctttc
     1141 tccaggtcgc acagcacggg ctccgcgccg tcggactcca cgtccggcag ttgctcctcc
     1201 ttgcggacca gcccccgcac ctcgtggccg tcttccgcca gcatccgcac cagatgccgc
     1261 gctacctggc cgtgcgctcc cgctaccagg actttcatgt gaggtctcct ctctggccta
     1321 ccgtcaagat cccttccaag atatcaactc ttgcgactcg ccgggacgcg gggataggac
     1381 aaaggccggg aatttcccgg cctttgtcca tacttatgtt gtcaagacga acagtctacg
     1441 gcaggaagcg ggagacggcg ctccacttgg cggactgtcc agccttctgc gagaggatgg
     1501 tcgtgatcgc gccgatggcg cctacgagcg cgaggttcac gttgcccagc acgttgacgc
     1561 ggcgcagaag cttcgacgcc tcctcgggcg tccgggctga aggggtagtc ccggactcta
     1621 tcggcacggc gccgtccggg gcctggcggg cgagcgagag cccgctgatt atgctggcga
     1681 gcccggtcag ggcggcggcg atgaacagcg cgtctttggt gaggacgagg ttgcgggcgt
     1741 cttcgtcgat ggactctccc gagatgccgg cccggccgac gaaccatgtc agggccgcgg
     1801 taccgaacgt gagcgcgttg accacgttgt agcggttcca ggcggcgttg agcagcttgc
     1861 cgcgatccgg ctcggaactc aggacatcga gcttcgggtt gagggcgagc ttgccgaaga
     1921 gagtcccccc aaaactggcg cctattccta ggttgtgggc gatccatgcg gcggtagtga
     1981 gtttagccat gacgaggctc cttccggttg actgcgcgtt agtttccccc gcccgcgttt
     2041 ctccaaacag ccccgccgga tacgccacga gaagggtacc ggttcaggcg ttcttcccct
     2101 gctttatgat gctctgaagc tcgccctgga gccgaccgac agcctgctcc ggcgcggtat
     2161 cgcccttgag gacgttgaag aactcctcgg ccatcttgag cgacatgtcc gagtagtaag
     2221 gggagacggg ccggggccgg gcggtcttca gggcctcctt gccgagcttc atcaccggga
     2281 ctttcttcag caactcctgg tcctcgtaag agactttcgt aacgggcagg cgggcgccct
     2341 cgatggcgcg catcttctgc tgctcggggg cggtgaagta tttgatcagg gcccacgccc
     2401 cgtccgcgtt cttgctcgtg gagtttatga agaggttggt cgcgccgagg gcgcttgaga
     2461 ggggcatgcc cttacgcgcc acgggcagag tcgagagccc gacctgctcc ggctttatct
     2521 tagactgagc cgggtccgag gtgagcgcgt agacgtagga ccagttgcgc atgaatacgg
     2581 cgtccccgcg caggaacgcg ccctgggcct cttcctcctt gtacgtcgcc atcgcctcgg
     2641 tggtgacgcc gtcggagacc atcgaatggt aggtggcgag accggcggcc gcctccgggg
     2701 agtctatgac gaccctgtcg cctttcagga cgtctccgcc gtgagtccag atgtactcca
     2761 gcccgtcgca aaccccgccc tcgtagttgg cgccctcgaa gacgaagccg aacctggtcc
     2821 cggagtcctg cttcaccttg agggccatct gcttgagttc gtcccaggtc tcgggcgcgc
     2881 tggtgaagcc gctcttttcg agaaggtctt tacggtagta gatcaggccg gccccggtca
     2941 cgaaggggac gccgtagatc ttgcccttgt agacgttgga ctcgacgttg ccctttatga
     3001 acttgtcttg caagcccttc gtgaaccggt ccgagaggtc catgagccag ccgttggagg
     3061 cgagctgggc gggccagatg acgtcgcccg cgatcacgtc tatgtcgctg ttccccgcct
     3121 gaagctccgt cctgagcttg tcgaagtagc cctgcgtgtc cgcgggcatc tcccgatgcc
     3181 tggccgtgta cttgcccttg tacttcttgt tgaagccgtc gagcagcttc ttgagcgtgc
     3241 cggtatcgtc cggcccctcg gcataagtga cggtcccgct cccgccttcc tctccaccac
     3301 cgcatcccgc caaagctccg ctgcccagca acgcggcgcc ggccagcccc gcgcccccaa
     3361 tccgcaggaa ctctccacgc ctgaagcgcc gcgttccacg aacagctccg cccctcgcat
     3421 ccatgctcat gcttcccctt cccgcacccc gtcacttttc ttagcaacaa ttgtaactca
     3481 gtaggcggca gagcggatag agccactata cttgggcgat gcacccgccg acgccgccga
     3541 cagagcgccc gcaccgcaac cacggtctct tctccgacca cttcctgaac gcgacgctcc
     3601 ccggacgccc ggactggcgg gcgctcgcgg acgaggcccg ccccgcaatg gaggatatat
     3661 cccgcctcct cgactcgtac acgccgagca ccaacgaggc gcaggtcgag gaggaactgg
     3721 tccggcccgt gctgcggctc ctcggacacg agcggagcta cgaggtgcag ccggcgctc
//
LOCUS       DASSMB010000232         4459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_825480, whole genome shotgun sequence.
ACCESSION   DASSMB010000232 DASSMB010000000
VERSION     DASSMB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825480"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VFJ72_13580"
     CDS             <1..518
                     /locus_tag="VFJ72_13580"
                     /inference="COORDINATES: protein motif:HMM:NF026109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HET7480534.1"
                     /translation="VEPVTELLAGTGVEIDNGVLVDEYCRTNIEGIYAAGDVANHYHP
                     VFGRHIRIEHWQNALRQGPAAARSMLGKGEPYEEIPWFWSDQYEHNLQYAGFHTEWDE
                     LVVRGSMKERTFVAFYRKDERVLAAVAINRGRDLRRSIPLIKAQRPVAASRLRDPEVD
                     LRTLVATAGKR"
     gene            743..1378
                     /gene="ribA"
                     /locus_tag="VFJ72_13585"
     CDS             743..1378
                     /gene="ribA"
                     /locus_tag="VFJ72_13585"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HET7480535.1"
                     /translation="MAVPDRARRVAVARLPTRHGEFDMIVYEAADLKEHVALAVGLVA
                     SEEPVLVRVHSECLTGDAFGSRRCDCGEQLEDSLEILQRAGRGVLLYLRQEGRGIGLT
                     SKVQAYAFQEIGLDTVEANLIQGLPEDSRDYRIAAEMLHDLGVRRVRLLTNNPAKVED
                     LRRHGVEISERLPLRVLPNPSNIRYLRTKRDRMGHLLPALATAEDIREDTP"
     gene            1966..2376
                     /locus_tag="VFJ72_13590"
     CDS             1966..2376
                     /locus_tag="VFJ72_13590"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HET7480536.1"
                     /translation="MSERTVEGACMCGAVRVLVRGEPFGAPYCHCKDCRKATGAPVSL
                     LVGYEEEQVQIEGTALQFYESSPGVHRSFCGRCGTSISYQDGRLPGEIYIHVGVLDDP
                     ASFEPTVHSWHSQRLDYMSIVDDLPRHRESSRPR"
     gene            2504..3061
                     /gene="efp"
                     /locus_tag="VFJ72_13595"
     CDS             2504..3061
                     /gene="efp"
                     /locus_tag="VFJ72_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET7480537.1"
                     /translation="MISTSQFRNGAAIRVDGKRFTILYFQHVKPGKGAAFVRTRLRNI
                     DTGATLEKTFRAGEKLDSVRTESRPMQYLYRDGDLFYFMDEDTYEQTAIPESVLGEAA
                     GFIVPNGTVNVLSADGEVVSVEPPAHVDLEVTETDPGLKGDTATGGSKPATLETGLTV
                     QVPLFVNVGDRVRVDTRSREYLTRV"
     gene            3095..3511
                     /gene="nusB"
                     /locus_tag="VFJ72_13600"
     CDS             3095..3511
                     /gene="nusB"
                     /locus_tag="VFJ72_13600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7480538.1"
                     /translation="MSRRTARKQAFLTLYQADLNEQPVSEVLARWRSYRGHLDEHAVS
                     LADGVERERAGLDAVLSEVSEGWPIHRMSVVDRAILRLALYEMLYVEDVPAEVAISEA
                     MEIAKGFSSEESPAFVGGVLRGAAARNPDKDRVGHG"
     gene            3504..4409
                     /locus_tag="VFJ72_13605"
     CDS             3504..4409
                     /locus_tag="VFJ72_13605"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HET7480539.1"
                     /translation="MDKSGMSGTAARWERHRQTFEAYLEGLYFSTEPRLAGLVEAMRY
                     SMLGGGKRVRPTLCMETAWVFEKDPAHVLPSAGAIELIHTYSLIHDDLPAMDNDDFRR
                     GRPTAHKKYGEAMAILAGDAFFGEALTLITVHQEGSCEQVVEVVRELADSTGVNGMVG
                     GQVIDMEQTGVGSEADPETLHMIHKYKTGALIKSSARIGAILAGVSEKQQTAISEYAN
                     ELGLCFQIVDDLLNATSTTAELGKSAGSDAEQGKATFVGVYGLDGARREADEAVKRAL
                     EALSRVDGDTSGLREMALFVRHRGS"
BASE COUNT          854 a         1306 c         1537 g          762 t
ORIGIN      
        1 gtgtcgagcc ggttacggag cttcttgccg ggaccggagt agagatcgac aacggcgtcc
       61 tggtagacga gtattgccgg acgaacatcg aaggcatcta cgcggcgggc gacgtggcca
      121 accactacca tccggtcttc gggcggcaca tccgcataga gcactggcag aacgcgctga
      181 gacaggggcc ggctgccgcc aggagtatgc tgggcaaggg agagccctac gaggaaatac
      241 catggttctg gtccgatcag tacgagcata acctccagta cgccgggttc cataccgagt
      301 gggacgagct cgtcgtgcgg ggcagtatga aggagcgaac cttcgtggcc ttctaccgaa
      361 aggacgaaag agttctcgcg gccgtggcta ttaaccgggg tagggacctg cgtcgctcga
      421 tcccgctgat caaagcgcaa cgtcccgtgg ccgcgtccag gctgcgggac cccgaagtcg
      481 atctgcgaac gctcgtggct acggcgggaa aacggtgagg ggttttggcg cagaacacgc
      541 aaggggtgag ccgctcgtgg ctcgtccaga atgccggcgg acgatgattg gaggaactaa
      601 agtggtcgga gcggaggtcg aagggcgcga tccgggccga gcgcgtcgcc ggtcgcgtgc
      661 gatcgagacc gaatgcaagg tggatgctgg gaagggcctg gcactccaac atccccccgt
      721 aagcggaccg aggggttgta aggtggccgt accagatcgc gcccggcgcg tcgcggtcgc
      781 ccggctgccg acccgccacg gcgagttcga catgatcgtg tacgaagcgg ccgacctcaa
      841 agagcacgtg gccctcgccg tcggtctggt agcgagcgag gagcccgtac tggtccgtgt
      901 ccactccgag tgcctgaccg gagatgcttt tggctcccgg cgctgcgact gcggagagca
      961 actcgaagac agcctcgaga tcctgcaacg ggcgggtcgc ggtgtcctgt tatacctgag
     1021 gcaagagggt cggggtatag gcttgacgag caaggtccag gcttatgcct tccaggagat
     1081 cggcctcgac accgtcgagg cgaacctgat ccagggcctg ccagaggact cacgggacta
     1141 ccggatcgcg gcggagatgc tgcacgacct tggggtacgt cgcgtccgtc tgctgaccaa
     1201 caaccccgct aaggtcgaag atctgaggcg ccacggagtc gagatatctg agcgcctccc
     1261 gctccgggtt ttaccgaatc cctcgaacat ccggtacctg cgcacgaagc gggacaggat
     1321 gggccacctc ctgcccgcgc tcgctacggc cgaagacatc cgggaggata cgccatgaga
     1381 cctgataaga gtgaggttgt cactacgttt gtctggctgt tgcggaacga cttgcacggg
     1441 gtgcctcggc cgccgaggca acttggggtc gcatgagcgg cgtgaaacgt tcgccgcagt
     1501 ggcggccgcc gttgccagag ggcggacgct tgatccgtcc tcctcggacg tcggtacccg
     1561 aaggcgagca gcatcgggtt gtggccggtc accaccagca acgggagatc tcccatgagt
     1621 ccgaagactt gaccgctcaa gcagttacaa tcctacgcca aaacgacatg ggtggctgga
     1681 cccgccacgc catcgatccc tattctcacc tgtggagccg ggactcggcg ttcatagcca
     1741 tcggtcacgc tctgttggat gcccgccgcg ccgccctcga actcgtacgc ttttcgctca
     1801 ccagtggaag accagcaagg tcccccatat cgtcttcaat cctgatgccc cgccgcacag
     1861 ctactatccc ggagcggagc accgggccta tccgcaatac gcttatggct gtatctctac
     1921 aaaaggtaga gtcgcgtctc tcttatccgc tcttgcgagg tcgcaatgag tgagcgaacg
     1981 gtagaggggg cctgcatgtg cggggccgtg agagtgttgg tgcgcggcga gcccttcggg
     2041 gcgccgtact gccactgcaa ggactgccgg aaggcgacag gagcgccagt ctcgctgctc
     2101 gttgggtacg aagaggagca ggtacagatc gaaggcacag cgctgcaatt ctacgagtct
     2161 tcgccggggg tacaccggtc cttctgcggg cgttgcggaa cttcgatctc ttatcaagac
     2221 ggccggttgc caggtgagat ctacatccac gtcggcgtcc tcgacgatcc ggcgtccttc
     2281 gagcctacgg tgcatagctg gcactctcag agactcgact atatgagtat tgtcgatgac
     2341 ctgccccggc accgtgagag tagccgcccc aggtaaacgg gcgggcgggg cctgacgggc
     2401 cggatggatc gggagcaatt atgcgataat atcgcggcgt ctgcgccctg cactgttggt
     2461 ggggcctcat ttgtagtgaa ggaatatcga ggggttgtaa gtcatgatat ccacgagcca
     2521 gttcagaaac ggggccgcca ttcgcgtgga cggcaagagg ttcaccatcc tgtacttcca
     2581 gcacgtcaag ccggggaagg gcgcggcgtt cgtgaggacg cgcctgcgca acatagatac
     2641 cggggcgacg ctggagaaga cattccgggc gggtgagaaa ctagactcgg tgcggacgga
     2701 gtcgcgcccg atgcagtacc tgtaccgcga cggggacctc ttctacttca tggacgagga
     2761 tacctacgag cagacggcga tcccggagag cgtgctcgga gaggctgccg ggttcatagt
     2821 cccgaacggg acggtcaacg tgctctcggc tgacggagag gtcgttagcg tcgagccgcc
     2881 ggcccatgta gacctggagg tgacggagac ggaccccggc ctcaagggag acacggcgac
     2941 cggcggcagc aagcccgcca cgctggagac gggcttgacg gtgcaggtgc cgctcttcgt
     3001 aaacgtcggt gatagggtgc gggtggatac gcgctccaga gagtacctta cgcgggtttg
     3061 atcttttgat ctttccggga gatcaggtgt cggtatgagt cgtcggacgg cccgcaagca
     3121 ggcgttcctg acgctatatc aggctgactt gaacgagcag cccgtcagcg aggttctcgc
     3181 cagatggcgc tcctaccggg ggcatctcga cgagcacgcg gtcagcctcg cggacggcgt
     3241 cgagcgggag cgggcgggac tcgacgcggt gctctccgaa gtctcagagg ggtggccgat
     3301 ccaccggatg agcgtcgtgg accgggcgat actcaggctc gcgctctacg agatgctcta
     3361 cgtcgaggat gtgccggcgg aggttgctat cagcgaggcg atggagatcg ccaagggctt
     3421 ctccagcgag gagtcgccgg ctttcgttgg aggagtgctg cgcggcgcgg cggccaggaa
     3481 cccggataag gacagagtcg ggcatggata agtcgggtat gtccgggact gcggctcgct
     3541 gggagagaca ccgccagact ttcgaggcgt atctagaagg gctttacttc tccacggagc
     3601 cgcgtctcgc ggggctcgtc gaagcgatgc gctactcgat gctcggtggt ggaaagcggg
     3661 tgcgccctac gctgtgcatg gagaccgcgt gggtcttcga aaaggacccg gctcacgtgc
     3721 tcccctcggc gggggcgata gagttgatcc acacttactc gctcatccac gacgacctcc
     3781 cggcgatgga taacgacgac tttcgccgcg ggcggccgac cgcgcacaag aagtacggcg
     3841 aggcgatggc tatactcgcg ggcgatgcgt tcttcggcga ggccctcacg ctcataaccg
     3901 tgcaccagga gggtagctgc gagcaggtcg tggaggtagt acgggagctc gcggattcga
     3961 caggagtgaa cggcatggtc ggcgggcagg tcatcgacat ggagcagaca ggggtgggct
     4021 ccgaggccga cccggagacg ctgcacatga tccacaagta caagaccggc gccctcatca
     4081 agtcctcggc gcgcatcggc gcgatactgg ctggagtgtc cgagaagcag cagacagcta
     4141 tctccgagta cgccaacgag ctcgggctgt gcttccagat agtggacgac ctcctcaacg
     4201 ccacctcgac gacggcggag ctcggcaaaa gcgcgggcag cgacgccgag cagggcaagg
     4261 cgacgttcgt gggcgtctac ggcctggacg gcgcgcggcg cgaggccgac gaagccgtca
     4321 aacgcgccct ggaagcgctc tcgcgggtgg acggcgacac gtcggggctg cgggagatgg
     4381 cgctcttcgt gcgccaccgg ggcagctaga gtctgctccg tccgatgaga gaagcgggac
     4441 agcgcctgga tctcctgct
//
LOCUS       DASSMB010000233         3639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_70004, whole genome shotgun sequence.
ACCESSION   DASSMB010000233 DASSMB010000000
VERSION     DASSMB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70004"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..811
                     /gene="purC"
                     /locus_tag="VFJ72_13610"
     CDS             86..811
                     /gene="purC"
                     /locus_tag="VFJ72_13610"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET7480540.1"
                     /translation="MAEDLLYEGKAKKVFATDDPQVLLHRFKDDATAFNAKKRGSWKD
                     KGKTNAAISAALFEYLESRGVPTHFIERVDDTTIKTKKLRMIPVEVIVRNVAAGSLAK
                     LLGYEEGRELKAPIVELCYKDDALGDPMLNWYHLRELGVSDEDLEFCEELGLKINEVL
                     APFFDDKGITLVDFKIEVGRDESGTPMLADEISPDTCRFWDKRTGERMDKDRFRRDMG
                     GVEEAYEEILRRVTDMGPRGTMD"
     gene            808..1032
                     /gene="purS"
                     /locus_tag="VFJ72_13615"
     CDS             808..1032
                     /gene="purS"
                     /locus_tag="VFJ72_13615"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET7480541.1"
                     /translation="MKVRVLVRPKEGILDPQGEAVKGALPALGFGGVTSVHIGRLIEM
                     EVESEDEVDAMCRRLLANPTIEEYEWEVVG"
     gene            1029..1691
                     /gene="purQ"
                     /locus_tag="VFJ72_13620"
     CDS             1029..1691
                     /gene="purQ"
                     /locus_tag="VFJ72_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET7480542.1"
                     /translation="MRIGVTVFPGSNCDRDALYAVERMGAEPVELWHADADLKGVDAV
                     IVPGGFSYGDYLRPGAIARFANVMGPLEEFARSGGPVLGVCNGFQVLTEAHLLPGALL
                     RNTGMRFVCDRVRVRVEATDTPWTTGCEPGEELTLPVAHNEGNYFADAETLARLEDEG
                     RVVLRYLENPNGSANDIAGVCNEGRNVVGIMPHPERVSDPALGSDEGLRILRSVLVGA
                     GV"
     gene            1757..>3639
                     /gene="purL"
                     /locus_tag="VFJ72_13625"
     CDS             1757..>3639
                     /gene="purL"
                     /locus_tag="VFJ72_13625"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET7480543.1"
                     /translation="MGRAPNYLELSLFSVMWSEHCGYKNSRPLLRRFPNEGARVLQGP
                     GENAGVVEIGDGWALAFKMESHNHPSAVEPYEGAATGVGGIIRDILAMGARPVALLDS
                     LRFGPLDEERNRYLFREVVAGIGGYGNAVGVPTVGGEVEFSRAYSGNPLVNAMCVGLI
                     RVEDLMSAQATGEGNLVVLIGSKTGRDGIHGATFASDELTEESESKRSNVQVGDPFCE
                     KMVIECCLKLLEQDLLVALQDLGAAGVTSSASEMAASGGVGIEIETDKVPLRETGMEP
                     WEVMISESQERMLAVVEPEKADQVLAMAERYGLVGAVVGRVADHGDLRVIHDGEVVGT
                     VPAEYLADAPVYERDVIRPKYLDQIPPLDLDLTAFPPPEDYNAALLKMLDHPNLCSRR
                     SIYRQYDHQVGTATVVLPGADAAVVRIKGTNLGFAVTTDGRGRHCYLDPRGGGAATVA
                     EAYRNLSCVGAEPVAVTDCLNFGSPEKRDGYYQLAECIEGMSEACEAFGTPVVSGNVS
                     LYNETDRGAIYPTPTVGMVGVMKDVNRHATPAFKRENDAIFVVGDFRPSLAGSDFLEI
                     LHGQVSGVPSEPNAEAEKAAADAVRRTIEAGIVDTAHDLSGGGLAAALAEMATAGGIG
                     AEV"
BASE COUNT          653 a         1078 c         1339 g          569 t
ORIGIN      
        1 gacgggctct tcgatccctc ctacgcgctg cggaacctgg gggtcgtgtt cgagcgggtg
       61 gaggagctga aggagaggtt gggcgatggc tgaggacttg ctctacgagg gcaaggcgaa
      121 gaaggtcttc gccacggacg acccgcaggt gctgctgcac cgcttcaagg acgacgcgac
      181 ggcgttcaac gccaagaagc gcggttcgtg gaaggacaag ggcaagacaa acgcggcgat
      241 cagcgcggcg ctcttcgagt acctggaatc caggggcgtg ccgacgcact ttatcgagcg
      301 ggtggacgac accacgataa agacaaaaaa actccgcatg atcccggtgg aggtcatcgt
      361 gcgcaacgta gccgctggct cgctggcgaa gctgctgggc tacgaggagg gccgggaact
      421 caaggccccg atagtcgaac tgtgctacaa ggacgacgct ctcggcgacc ctatgctcaa
      481 ctggtaccac ctccgggagc tcggggtatc ggatgaggac ctggagttct gcgaggagct
      541 ggggctcaag atcaacgagg tcctcgcgcc gttcttcgac gataagggca tcacgctcgt
      601 ggacttcaag atagaggtcg ggcgcgacga gagcggaacg ccgatgctgg ccgacgagat
      661 cagcccggac acctgccggt tctgggacaa gaggacggga gagcgtatgg acaaggaccg
      721 cttccggcgc gacatggggg gcgtcgagga agcctacgag gagatactgc gccgggtgac
      781 ggacatggga ccgcggggga cgatggattg aaggttcgag tcctggtccg cccgaaggaa
      841 ggcatcctcg acccgcaggg cgaggcggta aagggagcgc taccggcgct cggcttcggc
      901 ggtgtgacgt ccgtacacat cgggcgcctt atcgagatgg aggtcgagag cgaggacgag
      961 gtagacgcta tgtgcaggcg cctgctcgcc aacccgacca tcgaggagta cgagtgggag
     1021 gtggtcgggt gaggatcggg gtgacggtct tccccggatc gaactgcgac cgggacgccc
     1081 tctacgccgt cgagcggatg ggcgccgagc cggtcgagtt gtggcacgcc gatgcggacc
     1141 tcaagggcgt ggacgccgtg atcgtgccgg gcggattctc ctacggcgac tacctgcgac
     1201 ccggcgcgat agcccgcttc gcaaacgtga tgggaccgct ggaggaattc gccaggagcg
     1261 gcggcccggt cctcggcgtc tgcaacggtt tccaggtgct caccgaggcg cacctgctcc
     1321 ccggagccct gctccgcaac accgggatgc gcttcgtctg cgaccgcgtg cgcgtgcggg
     1381 tcgaggcgac cgatacgccc tggactacgg gctgcgagcc gggcgaggaa ctgacgctgc
     1441 ccgtcgccca caacgagggc aactacttcg cggacgcgga gacgctggcg cggctggagg
     1501 acgagggacg cgtggtgctc cgctacctgg agaacccgaa cggttcggcg aacgacatcg
     1561 cgggcgtgtg caacgagggc cgcaatgtcg ttggtatcat gccgcacccg gagcgggtct
     1621 cggacccggc gctcggctcg gacgaggggc tacggatact acgctcggtt ctggtgggag
     1681 caggggtctg aacatccagg agacatacac ggctctcggg ctctcggact ttgagtacga
     1741 ccgaatactg gagctgatgg gccgggcgcc gaactacctg gagttgtcgc tgttcagcgt
     1801 gatgtggagc gaacactgcg gctacaagaa ctctcgtccg ctactccggc gttttccgaa
     1861 cgagggtgcg cgggtgttgc agggaccggg ggagaacgcg ggcgtcgtcg agatcgggga
     1921 cggctgggcg ctggcgttca agatggagag ccacaaccac ccttcggccg tcgagcccta
     1981 cgagggggcg gcgacgggtg tcggcggcat tatccgggac atcctggcga tgggcgcgcg
     2041 gcccgtggcg ctgctggact ctttgaggtt cggacctctg gacgaggagc ggaaccgcta
     2101 tctgttccgg gaggtcgtgg cgggtatcgg gggttacggg aacgcggtgg gggtgccgac
     2161 ggtcggtggg gaggtggagt tctcgcgggc gtactcgggg aacccgctgg tcaacgcgat
     2221 gtgcgtcggg cttatccggg tggaggatct gatgagcgcg caggcgacgg gggaggggaa
     2281 cctggtagtc ctgatcggct cgaagaccgg ccgcgacggc atccacggcg ccaccttcgc
     2341 ctccgacgag ctgactgagg agtccgagtc caagcgctcc aacgtgcagg tcggcgaccc
     2401 gttctgcgag aagatggtca tcgagtgctg cctgaagctg ctggagcagg acctgctggt
     2461 ggcgttgcag gacctcgggg cggcgggggt tacgtcgtcg gcgtcggaga tggcggcgag
     2521 cggtggcgtc gggatcgaga tagagaccga caaggtcccc ctgcgcgaga ccgggatgga
     2581 gccgtgggag gtcatgatct ccgagtccca ggaacggatg ctcgccgttg tggagccgga
     2641 gaaagcagac caggtcctcg cgatggctga acgctacggt ctcgtcggcg ccgtcgtggg
     2701 ccgcgtggcg gaccatggcg acctgcgcgt catccacgac ggcgaagtcg tcggcaccgt
     2761 ccccgccgaa tacctcgcgg atgctcccgt ctacgagcgg gatgtgatcc gcccgaagta
     2821 cctcgaccag ataccgccgc tcgacctgga cctgacggcc ttcccgccgc cggaggacta
     2881 caacgccgca ttgctgaaga tgctcgacca tccgaacctc tgctcgcggc gctccatcta
     2941 ccgccagtac gatcaccagg tcggcacggc tacggtcgtc ctgcccggcg cggacgccgc
     3001 cgtcgtgcgg ataaaaggaa cgaacctcgg tttcgccgtt acgaccgatg gcagaggacg
     3061 ccactgctac ctggaccctc gtggcggggg agccgcgacg gtcgcggagg cgtaccgcaa
     3121 cctctcgtgc gtcggggcgg agccggtcgc cgtcaccgac tgcctcaatt tcggcagccc
     3181 cgagaagcgc gacggctact accagctcgc cgagtgcatc gaggggatgt ccgaggcgtg
     3241 cgaggcgttc gggacgccgg tcgtcagtgg aaacgttagc ctgtacaacg agacggatcg
     3301 cggcgcgatc tacccgacac cgaccgttgg aatggtcggc gttatgaagg atgtcaaccg
     3361 ccacgccaca cccgccttca agcgggagaa cgacgcgatc tttgtggtgg gagacttccg
     3421 gccctcgctg gccggatcag actttcttga gatcctgcac ggacaggtgt ccggcgtccc
     3481 ttcggaaccg aacgccgaag cggagaaagc cgcagcggac gccgtgcgga ggaccatcga
     3541 ggcgggcata gttgacacgg cccacgacct ctccggcggc ggactcgcgg ccgcgctggc
     3601 tgagatggcg accgcgggcg gtatcggggc ggaggtgca
//
LOCUS       DASSMB010000234         6340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1590419, whole genome shotgun sequence.
ACCESSION   DASSMB010000234 DASSMB010000000
VERSION     DASSMB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6340
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590419"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..478)
                     /locus_tag="VFJ72_13630"
                     /pseudo
     CDS             complement(260..478)
                     /locus_tag="VFJ72_13630"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            complement(579..1604)
                     /locus_tag="VFJ72_13635"
     CDS             complement(579..1604)
                     /locus_tag="VFJ72_13635"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HET7480544.1"
                     /translation="MDLRTLQQTARALVAEGKGILAADESFGTIEKRFKAVGIEATEE
                     SRRDYRQMLFTTPGIGEVLSGVILFDETIRQESSDGAPLPKILEDQGIIPGIKVDAGA
                     KEMALAPGEKVTEGLDGLRERLAEYRAMGARFAKWRAVITIGDGIPTRRCIRANAHAL
                     ARYAALCQEQDLVPIVEPEVLIDGDHTIEQCYEATEGTLRETFHELYEQRVELSGMLL
                     KPNMVISGKEAAEQSGVEEVARMTVECLRRSVPAAVPGIVFLSGGQSDKQATAHLNAM
                     NRDYENLPWEISFSYARALQEKPMETWAGNASNVEAAQKAFQHRLKMNGAARSGSYSE
                     EMERGAA"
     gene            complement(1713..1838)
                     /locus_tag="VFJ72_13640"
     CDS             complement(1713..1838)
                     /locus_tag="VFJ72_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480545.1"
                     /translation="MAEEARRRREEEDRRRREEEVRRTEKIEKVSAKKERELVRA"
     gene            2300..3244
                     /locus_tag="VFJ72_13645"
     CDS             2300..3244
                     /locus_tag="VFJ72_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480546.1"
                     /translation="MVTSRRVPSGSYARLAVVGSLVVIAAFVAASAVQAAVSISRAEI
                     SGTQLRIEGQAIANSTITVDGVAMGTSDGAGKFKIDRSGFTAPADCTVDVNDGSATAA
                     TARLSGCTVSSTPPPASSVSLSSLTVSPTDVVGGDPATGTVTLSSAAPTGGFVIDLSS
                     DNTAAATVPPSVTVPAGSTSATFTVSTKAVTNAQSAVIIGTVGGDFSTEKHAVITAWD
                     PFHFSNGSISILPGGNGSGRVTSQPAGIDCAITLGNGSGACSVFFPVGTVVKLDARAA
                     AGSAFVGWRTGLPGCFDPSKVTVARGTNITCQPGFVLK"
     gene            3490..5541
                     /locus_tag="VFJ72_13650"
     CDS             3490..5541
                     /locus_tag="VFJ72_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7480547.1"
                     /translation="MANRLANETSPYLLQHKDNPVDWYPWGEEALGRAREEDKPVLLS
                     VGYSACHWCHVMERESFEDEATARIMNQHFVNIKVDREERPDIDSIYMSALQAMTRGG
                     GWPMTVFMTPDGAPFYAGTYFPPVPSRGMPSFQQLLVSLADAYENRREDVLQSAESVR
                     DYLQASTGAAIPKAESAGSALLDAAAASLVSQLDRRFGGFGGAPKFPQAMNLEVLLRH
                     HHRTGDRDALSGVELTLRHMANGGIYDQLGGGFARYSVDEYWLVPHFEKMLYDNALLS
                     RLYLEAYEATGDPFYRRIAEETLDYVLRDMTSEEGGFYAAEDADSEGVEGKFYVWTPG
                     EVEAALDPEDAKLALRYWDVTERGNFEGKNILNVPRPPEAVAAEFGLSSEELWDRVVG
                     IRAKLLAAREKRVRPGRDEKVLAAWNGLMLRSFSLAARVTGREDYREAARKNAAFILE
                     KLKEGGRLHRSYKDGRARFNGYLEDYAMAADGLAALYEATFETRWLLEADAMCDAISE
                     LFWDEGSKSFYDTPADHEQLVTRPRDIYDSAAPSGTSVAVEVLLKLALLLDRNDYRQR
                     AETVLEELSGGMEKIPGAFGRLLCALDFSLGQTREIAIIGDPGDPDTQALMETVYSRY
                     LPNKVVAGRAPEDDEAAGLIPLLADRPLRGDRATAYVCEGYACKTPATEPEELADQLV
                     G"
     gene            5594..5902
                     /locus_tag="VFJ72_13655"
     CDS             5594..5902
                     /locus_tag="VFJ72_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480548.1"
                     /translation="MIAPPLQLPARDDAWKEWRDGAGKQERGGFEWLGRFLGRRRCEP
                     CAHLERLTRPGVLRGLVYRGGLRADISPADAYRPATAWKPLAFIIKPAAHGGVRHKQP
                     "
     gene            complement(5904..>6340)
                     /locus_tag="VFJ72_13660"
     CDS             complement(5904..>6340)
                     /locus_tag="VFJ72_13660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7480549.1"
                     /translation="AGQKDTLMQRYNDLERFPDVVPGLERLKEAGHAMIVFSNGTPRM
                     LEALMDAAELRPYFQGFVSVDEIGVYKPSPKVYRHVAQRLERPIGEVRLISSNPFDDI
                     GAEAAGMRAAWVDRSGGLFDTLGPPPEMVVGTLTDLADALEA"
BASE COUNT         1011 a         2158 c         2060 g         1111 t
ORIGIN      
        1 cgcctggtac ataacgcgcc caacatattc ctgcgagagg ccgggcgcat cacccaaatg
       61 agtgaacttt gtaattttta tgtgtcgccg agaagaatgc ccctgatgtt atgttcgaga
      121 ggcgctgatg agcagtcgca aaacaaccga ccgcgaggcg aggagcacgt aattcgtccc
      181 ggacgtgcct gttaatagcg gcattttggc agcgaggccc gccacaactg acgagccccg
      241 ctccctggtg tgtcggtggc tattggccgt tcgtaacgta gttggtgtcg aggtactcga
      301 tgatggcgtc ggactcgggc atcatcacgc cccgctcggc gtccacgagg aaggggacct
      361 ggcggctgcc ggagagcttc tccagtatct cggctgactt gctgccggac tcggcggagt
      421 gcgccaccca ggagagcccc ttctcgctca ggtgcgcccg gaccttcttg cagtaggggc
      481 agccttcttt ctgttagacc tcgatcatct tcgctccctc gcttcttgga ctcgcggaac
      541 cacccgagcc taacacgccg cgcgaagaag agagtatgtt acgcggctcc gcgttccatc
      601 tcctcggagt agctgccgga gcgggctgcg ccgttcatct tgaggcggtg ttggaaggct
      661 ttctgcgcgg cctcgacgtt cgaggcgttg ccggcccagg tctccatcgg tttctcctga
      721 agggcccgcg cgtaggagaa ggagatctcc cacggcaggt tctcgtagtc gcggttcatc
      781 gcgttgaggt gggcggtggc ctgcttgtcg ctctggccgc cggagaggaa gacgataccg
      841 ggcacggcgg ccggcacgct ccgccgcagg cactcgaccg tcatgcgggc gacctcctcg
      901 accccggact gctccgccgc ctccttgccg gagatgacca tgttcggctt gagcagcatc
      961 ccgctcaact ccacgcgctg ctcgtacagc tcgtggaagg tctcgcgcag ggtgccctcc
     1021 gtcgcctcgt agcactgctc gatggtgtgg tccccgtcta tgagtacctc cggctccact
     1081 atcgggacga gatcctgctc ctggcagagc gcggcgtagc gggcgagagc gtgcgcgttg
     1141 gcgcggatgc agcggcgggt gggtatgccg tcgccgatgg taatgaccgc ccgccacttg
     1201 gcgaaccgcg cgcccatcgc ccggtactcg gcgagcctct cgcgcaggcc gtcgagaccc
     1261 tcggtcacct tctcgccggg ggcgagggcc atctccttcg cgcccgcgtc caccttgatg
     1321 ccggggatga tgccctggtc ttcgaggatc ttcggcagcg gcgcgccgtc ggaggactcc
     1381 tgacggatcg tctcgtcgaa caggatcacg ccggagagca cctccccgat gccgggcgtg
     1441 gtgaacaaca tctgccggta gtctcggcgg ctctcctccg tcgcctcgat gcccacagcc
     1501 ttgaagcgct tctcgatggt cccgaagctc tcgtcggcgg cgaggattcc tttcccctcg
     1561 gccaccagcg cgcgggccgt ctgctgcagc gtccgtagat ccatgcctct ccttcctcct
     1621 gtcccacctg tgaggataac aaagagcccc ggttctcgaa gtccggggct ccctgtcgct
     1681 atgcggtttt cgtagtccct ttctagcgcc tgctaggctc ttaccagctc ccgctccttc
     1741 ttcgcgctca ccttctcgat cttctcggtc cgccggacct cttcctcccg gcgcctgcgg
     1801 tcctcttctt ctcgcctgcg acgggcctcc tccgccataa ggcgttctct gtaggagtag
     1861 cacatggctt ccgcctcctt gttagtgctt taccttacaa aattctatac ccccggcggc
     1921 ctcgggtaaa cggtcctcgg gagctttatg cgttagcggc gccaggcaac cgcttcgggc
     1981 aaccgcttcg ggctggcgtg ggcgcccgga gggcgtcgag caactcgttg gcatctactg
     2041 ggacgccttc ccggacatac acttccaaac cggaacggcc gcatggcacg gtctctcatt
     2101 ctgcgtgtat ctctctcccc tgtcctcgca taactattgt gcaaggccag ttgaggggtc
     2161 gcttcatggg gggtctcggt cgggcggctg cgtagtaacg caggttctcc gtgacactcg
     2221 aacgaaccaa tggctcgcgc cgcgcgtaag aaatagtgca aggtggtggg cgggagttct
     2281 gcccaggaag gagagcacta tggtcacgtc ccggagggtc ccgtctggct cctacgctcg
     2341 cctcgcggtg gttgggtctc tcgtcgtgat cgccgcgttc gtcgcggcct cagcagtaca
     2401 ggccgcggtc agcatctcgc gggcggagat cagcgggacg cagctacgga tcgagggcca
     2461 ggccatcgcc aacagcacga tcacggttga tggggtcgcc atgggcacga gcgacggcgc
     2521 cgggaagttc aagatcgacc gttccggctt tacggcgccc gccgactgca cggtagacgt
     2581 gaacgacgga tcggccacgg ccgcgaccgc ccgtctgtcc ggctgcacgg tcagttcgac
     2641 gccaccgccg gcctcgtcgg tatcgctctc ctcgctcacc gtcagcccga ccgacgtggt
     2701 cggaggggac ccggcgaccg ggaccgtcac cctttcctcg gccgcgccga ccggcggttt
     2761 cgtgatcgac ctgtcaagcg acaacacggc cgcggcgacg gtccctccga gcgtcaccgt
     2821 accggccggt tccaccagcg cgaccttcac ggtctccacc aaggcggtaa ccaacgcgca
     2881 gtcggcggta atcatcggga cggtgggagg agacttcagc acggagaagc acgcggtcat
     2941 cacggcgtgg gaccccttcc atttctcgaa cggaagcatc tcgatccttc ctggagggaa
     3001 cgggagcggg cgggtcacct cgcagcccgc ggggatcgac tgcgccatca ccctcggcaa
     3061 cggctcgggc gcctgcagcg tgttcttccc ggtcggcacg gtcgtgaagc ttgacgcccg
     3121 cgccgcggcc ggctcggcct ttgtggggtg gagaaccggc ctgccgggat gtttcgatcc
     3181 ctccaaggta acggtcgcgc gcgggaccaa tatcacctgc cagccgggat tcgtgctcaa
     3241 ataatcaacg gacttcgccc ctcgaacgcg gcgtctcgac agaacctgtg tctgcccatc
     3301 ggatatctct caccggcgtg tgatctctcg cgctctcgac cgcgctccga gcccgtccgg
     3361 gcgccgcgtt cgcactcgtt cgcattctga ggctcgtatc cgcgccaccg cgtgaagggt
     3421 atccgagacg ctcgctcgtg tggtacgtta gacatggcga aggcgcaccc acggcgagag
     3481 ggagacgaga tggcgaaccg tctggcgaac gagacgagcc cctacctctt acagcacaag
     3541 gacaaccccg tggactggta cccctggggc gaggaggcgc tcggccgggc gcgggaggag
     3601 gacaaacccg ttttgctgag cgtcggttac tccgcctgcc actggtgcca cgtcatggag
     3661 cgcgagtcct tcgaggacga ggccacggcc cggatcatga accagcactt cgtcaacatc
     3721 aaggtggacc gcgaggagcg gccggacata gactctatct acatgtccgc gctccaggcc
     3781 atgacgcgcg gcggcggctg gccgatgacc gtcttcatga cccccgacgg cgcgcccttc
     3841 tacgccggga cgtacttccc gccagtcccg tcccgcggca tgccctcctt ccagcagctc
     3901 ctcgtcagcc tcgccgacgc ctacgagaac cgccgcgagg acgtgttgca gagcgccgag
     3961 tccgtccgcg actacctcca ggcctcgacc ggcgccgcaa tcccgaaagc cgaatcggcc
     4021 ggcagcgcgc tcctcgacgc cgccgccgct tccctcgtct cgcagctcga ccggcgcttc
     4081 ggcggtttcg gcggcgcgcc caagttcccc caggccatga acctggaggt gctgctgcgc
     4141 caccaccacc gcaccggcga ccgcgacgcg ctctccggcg tggagcttac gttgcgccac
     4201 atggcgaacg gcggcatcta cgaccagctc ggcggcggct tcgcccgcta ctccgtggat
     4261 gagtactggc tcgtcccgca cttcgagaag atgctctacg acaacgcgct actgagtcgc
     4321 ctgtacctgg aggcgtatga ggcgaccggc gacccgttct accgccgcat cgccgaggag
     4381 accctggact acgtcctgcg cgacatgacc agcgaagagg gaggcttcta cgccgccgag
     4441 gatgccgact ccgagggcgt cgagggcaag ttctacgtct ggacgcccgg cgaggtagag
     4501 gccgcgctcg acccggaaga cgcgaagctc gccctgcgct actgggacgt cacggagcgg
     4561 ggcaacttcg agggcaagaa catcctcaac gtcccgcgcc cgcccgaggc cgtcgccgcc
     4621 gagttcgggc tttcgagcga ggagctgtgg gaccgcgtcg tggggatacg ggcgaagctc
     4681 ctcgccgcgc gcgagaagcg cgtccggccg ggacgggacg agaaggtcct cgccgcctgg
     4741 aacggcctca tgctccgctc cttctccctc gccgcccgcg tcaccggccg cgaggactac
     4801 cgcgaagccg cgaggaagaa cgcggcgttt atcctggaga agctcaagga gggcggccgt
     4861 ctccaccgct cgtacaagga cggccgggcg cgcttcaacg gctacctgga ggactacgcg
     4921 atggccgccg acgggctcgc ggctctgtac gaggccacgt tcgagacacg ctggctcctg
     4981 gaggccgacg ctatgtgcga cgcgatctcc gagttgttct gggacgaggg gagcaagagc
     5041 ttctacgata cgccggcgga tcacgagcaa ctcgtgacgc gcccgcgcga catctacgac
     5101 agcgccgcgc cctccggcac ctccgtcgcc gtcgaggtgc tgctaaagct cgccctcctc
     5161 ctcgaccgga acgattaccg tcagagggcc gagacggtgc tggaagagtt gagcggcggg
     5221 atggagaaga tccccggcgc ctttggacgc ctgctctgcg ccctcgattt ctcgctgggc
     5281 cagactcgtg agatcgctat tatcggcgat ccaggcgatc ccgatacgca ggccctcatg
     5341 gagaccgtct actcacgcta tctgccaaac aaggtcgtcg cgggccgcgc cccggaggac
     5401 gacgaagccg ccggcctcat cccgctcctc gccgaccggc cgctgcgcgg ggatcgggcg
     5461 accgcatacg tctgcgaggg ctacgcctgc aagaccccgg cgacggagcc ggaggagttg
     5521 gcggatcagc tagtaggcta ggggtcatgg ggcgcggggt ccggcgccgc caatacgtgc
     5581 cggcgtttgc gccgtgatcg caccgccgct acagttgccc gcgcgagacg atgcgtggaa
     5641 ggagtggcga gatggcgccg gtaaacaaga gagaggcggc ttcgagtggc tggggcggtt
     5701 cctcggccgg cgccgctgcg agccgtgcgc gcacctggag aggctcacgc gccccggagt
     5761 gctgcgtggc ctggtgtacc gcggcggact gcgcgccgac atatctccgg cggacgcata
     5821 caggccggcg accgcgtgga agccgttagc cttcatcatt aaacccgcgg cgcacggcgg
     5881 ggtgcggcac aaacaaccgt gagctatgcc tccagagcgt ccgcgagatc ggtgagcgtc
     5941 ccgacgacca tctccggcgg cggaccgaga gtgtcgaaga ggccgccgga gcggtccacc
     6001 cacgcggccc gcatgccggc ggcctccgcc ccgatgtcgt cgaacgggtt ggacgagatg
     6061 agccgcactt ccccgatggg tcgctccagc cgctgcgcga cgtgccggta gaccttcggg
     6121 gaaggtttgt agacgcctat ctcatcaacg ctcacgaacc cctggaagta cgggcgtagt
     6181 tccgcggcgt ccatgagcgc ttcgagcatg cggggggtgc cgtttgagaa gacgatcatg
     6241 gcgtgaccgg cctccttgag gcgctccagc cccggtacga cgtcggggaa gcgctccagg
     6301 tcgttgtagc gctgcatgag cgtgtccttc tgcccggcgt
//
LOCUS       DASSMB010000235         5028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_327341, whole genome shotgun sequence.
ACCESSION   DASSMB010000235 DASSMB010000000
VERSION     DASSMB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327341"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFJ72_13665"
     CDS             complement(<1..359)
                     /locus_tag="VFJ72_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7480550.1"
                     /translation="MIKDSVIKDPSLAPEGHRKIDWAAQHAPVLNKIAKEQLADGSLR
                     GRKIAMTIHLEAKTAYLACLLHEAGADVTVSGSNPLSTQDDVCAALAERGVRVYATHD
                     PSDEDFERYLHQTIETGP"
     gene            543..842
                     /locus_tag="VFJ72_13670"
     CDS             543..842
                     /locus_tag="VFJ72_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480551.1"
                     /translation="MSYLGIQTFWGLCVLLTPVALGLYPSRLWAVPLCLVTIANGLHL
                     TVFRHEYNALLRKTVHLIPYARYVTPAQRDPKYFLPFGLAYTLFGVISALMVILH"
     gene            complement(829..1695)
                     /locus_tag="VFJ72_13675"
     CDS             complement(829..1695)
                     /locus_tag="VFJ72_13675"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET7480552.1"
                     /translation="MISAFTALMAVATLRREKPRVIQRDWPVNLAHRGASARAPENTL
                     EAFRLALQSGAGGLEMDAHMTRDGRLVVIHDDTVDRTTDGFGAVREMTLGEIRSLDAG
                     YRFSPDDGLTHPYRGLGLMVPTLEEVYQSFPETPINVEIKEDQAGVEEAVLRVIEEAA
                     AEDRTIVAAAIHEVVDRFRRVSEGRVPTAASRREIGWFFAFARLYLESLLRPAYAALQ
                     VPTEHRRIPLATPRFLAAAHNRNVRVDVWTINDPDEMRRLLDLGADTIMTDRPEELTK
                     VLEERRSNLNAE"
     gene            1777..2499
                     /locus_tag="VFJ72_13680"
     CDS             1777..2499
                     /locus_tag="VFJ72_13680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET7480553.1"
                     /translation="MLRRVLLATILIVIFASCSAQSAPVQRPPMTYVSLGDSLAVGVG
                     SSEPDHRGYAPLLRERFSRDTGREVRLVQLGVSGETSASLVGGYPDRETSQLARAREA
                     LKEYPGATVTLSIGGNDLLRTAGEDDQARRDAVSRYGQNLDFILKTLNGSSRPAPRIT
                     VLALYNPAPGSFTDKWSRRLNDEIRTTASRNGVAVADGDRTFEGHAAQYTHYTRETPD
                     IHPTDRGYEALTRAFARTFSKA"
     gene            2596..2680
                     /locus_tag="VFJ72_13685"
     tRNA            2596..2680
                     /locus_tag="VFJ72_13685"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2630..2632,aa:Leu,seq:gag)
     gene            complement(2860..3315)
                     /locus_tag="VFJ72_13690"
     CDS             complement(2860..3315)
                     /locus_tag="VFJ72_13690"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480554.1"
                     /translation="MRSYVVLFVSVAAITLILTGVALAATIKCTGGECQGTNMRDTLY
                     GTPVNDIIFGNGDNDHLYGGGGMDSLKGGPGNDRQYGSVGDDRVKGGPGSDTLYGGDG
                     NDIMRGGLHNRTNDGAPDVINCGAGRDTVYFTPGVDTINGCEIRNPSTP"
     gene            complement(3391..3615)
                     /gene="secG"
                     /locus_tag="VFJ72_13695"
     CDS             complement(3391..3615)
                     /gene="secG"
                     /locus_tag="VFJ72_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7480555.1"
                     /translation="MVYVLAFFHTVLCVALVGLILLHSGKGGGLSSSLGGGLGGTFSG
                     TTIMEKNLTRLTMIVVGLFALSIVLLIFFG"
     gene            complement(3649..4434)
                     /gene="tpiA"
                     /locus_tag="VFJ72_13700"
     CDS             complement(3649..4434)
                     /gene="tpiA"
                     /locus_tag="VFJ72_13700"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7480556.1"
                     /translation="MARRPMMAANWKMNKTVREAQNYTAALLPHAAEAEGVDVAVFAP
                     LTCLSEVARMSEDSAVIAGAQNFYYEDSGAFTGEVSVPMLQDIGVRAVIVGHSERREV
                     FGESDDLVARKTKRALEAGLIPVVCVGETKEERDGGGMWDKISGQVQAVMDALDDPDG
                     DVLVFAYEPIWAIGTGDTATPEDAQDAIGQMRGQLKELAGDDFAESVRILYGGSVKPG
                     NVAEIMAQDDVDGALVGGASLEVESFVSLIEAAAETAPEVESE"
     gene            complement(4465..>5028)
                     /gene="pgk"
                     /locus_tag="VFJ72_13705"
     CDS             complement(4465..>5028)
                     /gene="pgk"
                     /locus_tag="VFJ72_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7480557.1"
                     /translation="VIESLLGVADTLLIGGAMCFTFFKAQGYEIGNSLVEDDYIAEAN
                     RLMGEAGDKLVLPTDVVIAQKMEEGAQSRAVGVDEIPAGWMGLDVGDATVGLFREHIS
                     NAKTIFWNGPMGVFEMEAFAKGTEGVARAVAESGATSVVGGGDSVAAVGKLGLEDEMS
                     HISTGGGASLEYIEGKELPGVAVLPGK"
BASE COUNT          879 a         1716 c         1431 g         1002 t
ORIGIN      
        1 gggccggtct ctatggtctg gtgcaggtag cgctcgaagt cttcatcgga agggtcgtgg
       61 gtcgcgtaga cccggacgcc gcgctccgcc aacgcagcac agacatcgtc ctgggtcgag
      121 agcgggttac tcccggatac ggtcacgtca gcgccggctt cgtgtagcag acacgctaga
      181 taggccgtct tggcttccag gtggatagtc atggcgatct tgcgtccccg cagcgagccg
      241 tcggcgagct gctccttcgc tatcttgttc aggaccggcg cgtgctgggc ggcccagtcg
      301 atcttgcggt gtccctccgg cgcgagagac ggatctttta tgacggaatc tttgatcatg
      361 ggttcgtggc tccttttcgg gatgcttcgg gcaacgctcg aatattttct actgcgcgca
      421 tagtttacag atgaggcggg cgggattccg tgagcacgat acctgctcgt atagtcgctc
      481 tgggacgggt agaatctttt accgtcagaa tgcggcacag ataccggata aagatggcat
      541 ggttgagtta cctggggatc cagaccttct gggggctctg cgtcttgcta acccccgtag
      601 ctctaggact ctatccatcg aggctctggg cggtgcctct gtgtttggtc acgattgcca
      661 acggcctaca cctgaccgtc ttccgccacg agtacaacgc gctgctgcgg aagacggtac
      721 acctgatccc ctacgcccgc tacgtcaccc cggcccagcg cgaccccaag tacttcctgc
      781 ctttcggcct cgcctacacc ctattcggcg tgatctccgc cctgatggtc attctgcatt
      841 aaggttgctc cggcgctcct ccagtacttt cgtcaattcc tccgggcggt cggtcataat
      901 ggtgtccgcg ccgaggtcca ggagccgccg catctcgtcc gggtcgttta tcgtccacac
      961 atccacccgc acattccggt tatgcgccgc cgcgaggaag cgaggcgttg cgagcggtat
     1021 cctccgatgc tccgtcggca cctgaagggc cgcgtaagcc ggacggagca gggactccag
     1081 atacagccgg gcaaaagcga agaaccaccc gatctcccgc cgggacgccg ccgtcgggac
     1141 tctaccttcc gacacgcgcc ggaaccgatc caccacctcg tgtattgctg cggcgacgat
     1201 agtcctgtct tccgccgccg cctcctctat aacacgcagc acggcctctt cgactcctgc
     1261 ctgatcctcc ttgatctcca cgtttattgg tgtctccggg aaactctggt agacctcctc
     1321 aagcgtgggc accataagac ccaacccccg ataagggtgg gtcagaccgt catccgggct
     1381 gaagcggtat ccagcatcca ggcttcttat ctcgccgagc gtcatctccc gcacggcgcc
     1441 gaagccgtct gtagtccggt ccacggtgtc gtcgtggatt accacgaggc ggccgtcgcg
     1501 ggtcatgtga gcgtccatct ccagcccgcc cgcgccgctc tgcagtgcca ggcggaaggc
     1561 ttcgagggtg ttctccggcg cccgggcgga ggctccccgg tgagccaggt taacaggcca
     1621 atctctctga atgacccgcg gcttctccct gcggagcgtc gcaacagcca taagagcggt
     1681 aaacgcggag atcacgccaa gcagtatcca ctttctgcgc atccgtagaa cttagcacac
     1741 gaaatgcccg gtctctctgg tagactcccg cgagtcatgc tccgaagagt tctcctcgcg
     1801 actattctga tagtgatctt cgcctcctgc tccgcgcaga gcgcgcccgt acagcgtccg
     1861 cccatgacct atgtctccct gggcgactcg ctcgccgtcg gcgtcggttc ttcggaaccg
     1921 gatcaccgtg gttacgctcc gctgctgagg gagagatttt cccgcgatac cggacgcgaa
     1981 gtgcgtctcg tccagctcgg cgtgagcggc gagactagcg cgagcttggt gggcggatat
     2041 cccgacagag aaacctccca gctcgcccgt gccagggaag ccctcaagga gtatcccggc
     2101 gccaccgtga cgctctccat aggcggcaat gacctcttga gaaccgccgg cgaagacgat
     2161 caagcccgcc gggacgccgt ctcgcgttac ggccagaacc tcgacttcat cctcaagacg
     2221 ctcaatggct cctcgcgtcc tgcgcccagg atcaccgtcc tcgcgctcta caatcccgct
     2281 cccggcagct tcaccgacaa atggagcagg aggctcaatg atgagatccg tacgaccgcc
     2341 agccgtaacg gcgtcgccgt ggcggacgga gaccggacat tcgagggtca cgcggcgcaa
     2401 tacacccact acacccgaga aaccccggac atacacccca cggaccgcgg ttacgaggcc
     2461 ctgacccgcg cgttcgcaag aaccttcagc aaggcgtaaa taatccgaca tccgggacac
     2521 cggagaacca tcggacgatc acctcagcct cgttgaccct tcaaggggtg tcccttatac
     2581 tctgacggct gctttgtcgg agtggcggaa ttggtagacg cgctaggttg agggcctagt
     2641 gcccgataag ggcgtggggg ttcgagtccc ccctccgaca catctatcgt tgttcatcgc
     2701 gggatgacga attggccgga gccgccccgg cactttctct gtaagtcgtt atacgctgaa
     2761 ctatatagcg gggtgtataa ttacgtccgg agtcgcctgt tctccccatc ctccccgggc
     2821 ggctcctttc tcgtgcgcag tttgtagggc tgcgtgctct tacggagtgg aggggtttct
     2881 tatctcgcag ccgtttatag tatctacgcc tggcgtaaaa tacaccgtgt ctctgccggc
     2941 gccgcagtta atgacatcgg gtgctccgtc gttcgtccgg ttatggagac cgccgcgcat
     3001 aatgtcgtta ccatcaccgc catagagcgt gtcactaccg ggaccgccct tgaccctgtc
     3061 gtcacccacg gacccgtact gcctgtcgtt gcccggtccg cccttcagcg agtccatgcc
     3121 gccaccgccg tacagatgat cgttgtcacc gttgccaaag attatgtcgt tcacgggcgt
     3181 gccgtagagc gtgtcgcgca tattcgtccc ctggcattcg ccgcctgtac atttgatggt
     3241 cgccgcgagc gcaacccccg taaggatcag cgtaattgcc gccacggaca cgaacaagac
     3301 aacgtatgat ctcactagtt acccctctcg acgcagatgt acgaacacct gctcctgtga
     3361 tcggaccgga ccgacgaaga ctttagcccg tcagccgaag aagatgagga gcacgatgct
     3421 caacgcgaag agaccgacca ctatcatggt cagacgggtc aggttcttct ccatgatggt
     3481 cgtaccggag aaggtgccgc ccagaccgcc cccgagcgag gaggagagcc ccccaccctt
     3541 gccggagtgc agcaggatca ggccgacgag cgccacgcag agcaccgtat gaaagaacgc
     3601 gagaacgtat accatgcgct ttactctacc acaccggccc gtactgctct actcactctc
     3661 gacctccgga gccgtttcgg cggcggcttc tatcagggag acgaacgact ccacttccag
     3721 gctcgccccg ccgacgagcg cgccgtccac atcgtcctga gccatgatct ccgcgacgtt
     3781 gcccggcttg accgagccgc cgtaaaggat tcggacgctc tccgcgaagt cgtctccagc
     3841 gagttctttt agctggcccc gcatctggcc tatcgcgtcc tgcgcgtcct ccggcgtcgc
     3901 cgtatcgccc gtgccgatgg cccagatcgg ctcgtaggcg aagacgagaa cgtccccgtc
     3961 ggggtcatcg agcgcgtcca taaccgcctg gacctggccg gagatcttgt cccacatccc
     4021 gccgccgtcg cgctcttctt tcgtctctcc cacgcagacg accgggatca ggccagcctc
     4081 cagcgcccgc ttcgtcttcc tggcgaccag gtcgtccgac tcgccgaaga cttcgcgccg
     4141 ctcggagtgt ccgacgatga ccgcccgcac cccgatgtcc tggagcatcg ggaccgagac
     4201 ctctccggtg aacgcgccgg agtcttcgta gtagaagttc tgcgccccgg cgattaccgc
     4261 cgagtcttct gacatccggg cgacctcgga gaggcacgtc agcggcgcga agaccgcgac
     4321 gtccaccccc tcggcctcgg cggcgtgcgg tagcaaggcg gccgtatagt tctgcgcctc
     4381 ccgcacggtc ttgttcatct tccagttggc cgccatcatc gggcgtcgtg ccatgagctg
     4441 ttctcccctc tccggctctg ttgtctactt gccgggcagg accgccacgc cgggcagctc
     4501 cttgccctcg atgtactcca gcgacgctcc accgccggtg gagatgtggc tcatctcatc
     4561 ctccaggccg agcttgccga cggcggccac ggagtcgcca cccccgacga cgctcgtcgc
     4621 gccgctctcg gcgaccgccc gcgccacgcc ctcggtgccc ttcgcgaagg cttccatctc
     4681 gaagacgccc atcgggccgt tccagaagat ggtcttcgcg ttcgagatgt gctcccggaa
     4741 gagacctacg gtcgcgtctc ctacgtccag tcccatccag cccgcgggga tctcgtctac
     4801 accaacggca cggctctggg caccctcttc catcttctgg gcgattacca cgtccgtcgg
     4861 gaggacgagc ttgtctccag cctcgcccat gagacgattc gcctctgcga tgtagtcgtc
     4921 ctcgacgagc gagttcccga tctcgtaccc ctgcgccttg aagaacgtga agcacatcgc
     4981 gccgccgatg aggagcgtat ccgccacgcc gagcaggctc tctattac
//
LOCUS       DASSMB010000236         2647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1211988, whole genome shotgun sequence.
ACCESSION   DASSMB010000236 DASSMB010000000
VERSION     DASSMB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211988"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VFJ72_13710"
     CDS             <1..361
                     /locus_tag="VFJ72_13710"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HET7480558.1"
                     /translation="EEALMLDPTGAVATCNATNFFIVRRGEVWTSTGHYCLNGITRRM
                     VIELCRANGIPVRERAFSLTDVYGADEAFVTGTFGSLTPVVEVDGRGIGDGRRGELTA
                     RLSELYADAVSRATSGS"
     gene            365..1093
                     /locus_tag="VFJ72_13715"
     CDS             365..1093
                     /locus_tag="VFJ72_13715"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480559.1"
                     /translation="MADCLKLSVWSGPRNVSTALMYSFRQRSDTLVVDEPLYGHYLKT
                     TGADHPGDDEVMERMDCDGERVVRETILGPCERPVHFFKNMAHHLAGLDRGFLSRITN
                     VLLIRAPEEVLPSLATQLHDPTLRDTGFAWQVEILQTVLEQGRDPLVLDSKQLLLDPP
                     GVLREACARLGLPFEDAMLRWPAGPKPEDGVWAKHWYANVRASTGFMAYRPKTEPPPE
                     RLRPLLEECAPLYERLRRYAIAAG"
     gene            complement(1111..2199)
                     /locus_tag="VFJ72_13720"
     CDS             complement(1111..2199)
                     /locus_tag="VFJ72_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7480560.1"
                     /translation="MTDKIVFTRGVPPPEAFPAKELGECFEAAVLEDTAVVLQYGHQQ
                     GYAPLREELAREYGVSPANVMVGNGSLQLQDLVAAHLVRPNGVVYTEQPSYDRAILTF
                     RRRGARVVGIPLEEDGISTDRLEAALAREVPAFLYLVPDFQNPAGATLSLEKRRRVVE
                     LADEYGFTVIEDVPYRKLRYRGEDLPLLREIDDSNVITMSSFSKLLSPGIRVGFMLGP
                     EELVRDLTRLGENTYLSPVLPTQAAVAEYLRRGWLQPNIEKLKDLYRPRWQATEEAVR
                     RELPDAQAFIPDGGFFVSVMLPEEANTDDLVGRAKEIGLVLTPGAAFFADPDDEGATP
                     GDRFVRLPFCAVTPGQISEGVSRLASLL"
     gene            complement(2306..2557)
                     /locus_tag="VFJ72_13725"
     CDS             complement(2306..2557)
                     /locus_tag="VFJ72_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480561.1"
                     /translation="MRPAFGSGEDWNFASRPWPCKVSRLRPAFGSGEDWNIAALAVRG
                     GQMSGLRPAFGSGEDWNYINMRKKGWTCVIVAIVLGPGF"
BASE COUNT          459 a          971 c          790 g          427 t
ORIGIN      
        1 cgaagaggcc ctgatgctcg accctaccgg cgccgtagcc acctgcaacg cgaccaactt
       61 ctttatcgtg cggcgcggcg aggtctggac ctccaccggc cactactgcc tcaacggcat
      121 cacccgccgc atggtcatcg agctgtgcag ggcgaacggc atcccggtgc gcgaacgggc
      181 cttctccctc accgacgtct acggcgcgga cgaggcgttc gtcaccggaa ccttcgggag
      241 cctgacgccg gtcgtggagg tggacgggcg cgggatcggt gacggcagac gaggcgaact
      301 tacggcccgc ctctcggagc tgtacgccga tgccgtctcc cgcgccactt cggggtcata
      361 ggctatggcc gactgcctca agctcagcgt gtggtccggg ccgcgcaacg tctcgacggc
      421 gctcatgtac tcgttccggc agcgttcgga cacgctggtc gtggatgaac cgctctacgg
      481 gcactacctc aagacgaccg gcgcggacca tccgggtgat gatgaggtaa tggagcggat
      541 ggactgcgac ggggagcggg tggttcggga gaccatactc ggcccgtgcg aacgtccggt
      601 ccacttcttc aagaacatgg cccatcacct ggccggtctg gaccgaggct tcctctcacg
      661 tattacgaac gtgctcctga tccgagcccc ggaagaggtg ctgccgtcgc tcgcgacgca
      721 actgcacgac cccaccctgc gagacaccgg ctttgcctgg caggtcgaga tcctgcagac
      781 cgtcctggag cagggccggg atcccctcgt cctcgactcg aagcaactac ttcttgaccc
      841 gcccggcgtc ctgcgcgaag cctgcgcccg gctcggcttg ccgttcgagg acgcgatgct
      901 gcgctggccc gccggaccga agccggagga cggcgtgtgg gcgaagcact ggtacgccaa
      961 cgtccgcgcc tcgaccggct tcatggcgta ccgcccgaag acggagcccc cgccggagcg
     1021 gctgcgtccc ctgctggagg agtgcgcgcc gctctacgag aggctccgcc gctacgctat
     1081 cgcggcggga taggccgagt ccatacggcg ctacagcagg ctcgccagac ggctgacacc
     1141 ctctgagatc tggccgggag taaccgcgca gaacgggagc cgcacgaacc ggtctccggg
     1201 cgtcgcgccc tcgtcgtccg ggtcggcgaa gaacgccgcg ccgggcgtga gcaccagacc
     1261 gatctccttc gcccgcccta ccagatcgtc cgtgttagcc tcctccggca gcatgacgct
     1321 cacgaaaaag ccgccgtccg ggatgaacgc ctgcgcgtcc ggcagctccc ggcgcaccgc
     1381 ctcctccgtg gcctgccagc gcggccggta gagatccttc agcttctcga tgttcggttg
     1441 cagccatccc cgccgcaggt actccgcgac cgccgcctgc gtcggcagca ccggcgagag
     1501 gtaagtgttc tccccgaggc gggtcaggtc ccgcaccagt tcctccggcc cgagcatgaa
     1561 cccgacccgg atgccggggc tgagcagctt gctgaacgag ctcatagtga tgacgttcga
     1621 gtcgtcaatc tcgcgcagga gcggcaggtc ctcgccccgg tagcgcagct tgcggtacgg
     1681 tacgtcctcg atcacggtga agccgtactc atccgccagc tctaccaccc tccgccgctt
     1741 ctccagcgag agcgtcgccc cggccgggtt ctggaagtcc ggcaccaggt acaggaaagc
     1801 cggcacctcc cgcgcgagcg ccgcttcgag ccggtccgtg ctgatcccat cctcctccaa
     1861 cgggatgccc acgacccgcg ccccacgccg ccggaaggtg agtatggccc ggtcgtagct
     1921 cggctgctcc gtgtagacga ccccgttcgg ccgcaccaga tgagccgcca ccaggtcctg
     1981 caactgcaat gacccgttcc cgaccatcac gttcgccggc gagaccccgt actcccgcgc
     2041 cagctcctcg cgcagcggcg cgtagccctg ctggtgcccg tactgcaaca ccaccgccgt
     2101 gtcctccaga acggccgcct cgaaacactc tccaagctcc ttcgccggga acgcctccgg
     2161 tggcggcacc ccacgagtaa agactatttt atcggtcaag atcctaactc cttatctatc
     2221 tctccaaagg tacgcatcct agtccgagat acaggtggta cgcaactgcc tgaattacat
     2281 ataggcgttt caatccctgc ccagcctaaa aaccggggcc aaggacaatc gccacgatta
     2341 cgcaagtcca acccttcttc cgcatgttga tgtagttcca atcctcgccc gacccgaagg
     2401 ccggacgcaa ccccgacatc tgcccgccgc gcaccgccag ggccgcgatg ttccaatcct
     2461 cgcccgaccc gaaggccgga cgcaacctcg acaccttgca gggccacggc ctcgacgcga
     2521 agttccaatc ctcgcccgac ccgaaggccg gacgcaacct ttacggacat cggaggcgtc
     2581 ggctatctag atgagttcca atcctcgccc gacccgaagg ccggacgcaa cccccattag
     2641 ttcggcc
//
LOCUS       DASSMB010000237         6283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_327925, whole genome shotgun sequence.
ACCESSION   DASSMB010000237 DASSMB010000000
VERSION     DASSMB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327925"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFJ72_13730"
     CDS             <1..924
                     /locus_tag="VFJ72_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET7480562.1"
                     /translation="VRDSTFDRYEGAVRLHIKPALGRLKLKKLTPAHVQSFYRDRLDA
                     GCAQASVNKMHVVLHKALKQAVEWHMVPRNVAGAVKAPRPAPREMHPLSAEETRRLLE
                     AARGDRLEALYVLAVHTGMRQGELLALKWQDVDLENATMSVRRTVTKSGGKLLLGEPK
                     TKKSRRTIQLTGAAVRALRGHLDRQMKEIEHLGNLYRDDGLVFTSEVGTIINPTNLRK
                     RSFASLLKKARLPGIRFHDLRHTCATLLFGRNVHPKYVQELLGHSNVGITLDTYSHVI
                     PGMGDHAARAMEDALSRPVPRPSRLASPPME"
     gene            complement(1026..1098)
                     /locus_tag="VFJ72_13735"
     tRNA            complement(1026..1098)
                     /locus_tag="VFJ72_13735"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1063..1065),aa:Ala,seq:tgc)
     gene            1210..1458
                     /locus_tag="VFJ72_13740"
     CDS             1210..1458
                     /locus_tag="VFJ72_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7480563.1"
                     /translation="MALYEYKCAECEERFDLMRSMSAADEPAECPECGSVESRRLISN
                     FASITPGASALNTNPMMDARIASGNAAGGCCGGGCGCH"
     gene            1463..1852
                     /locus_tag="VFJ72_13745"
     CDS             1463..1852
                     /locus_tag="VFJ72_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET7480564.1"
                     /translation="MSADTEQVPDLVLKGIEEFNRGEFFECHEYLEEAWMQEPRRVRF
                     LYQGILQVGVGFYHLKNGNWRGATGLLRNGTARLREFEPDAMGIDVGRLIRESERCLA
                     ELEELGRERVGEFDLSAIPRVEWVQER"
     gene            complement(1918..3030)
                     /locus_tag="VFJ72_13750"
     CDS             complement(1918..3030)
                     /locus_tag="VFJ72_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET7480565.1"
                     /translation="MPGFLHRFELAFGRLWSEGGQVAQKALAAGVAYWVASALLGHNQ
                     PAFAPIAALISLSVVNGREWRQAVELVFGVACGLTVADLLLSLVGSGALQIALVVGTA
                     TAIVTFFGGGALSITEAGVSALLMVILASPSSGASGDRFVEALVGSGLALAIRAILPN
                     NPGHTVAQAAHPIFDEFIGVLEEIYEALYTRDIEQAEEALVKARHMDARVGGFKEALE
                     NGYGTVRLSPARRRALGQLGFYAAAADQLDLAIRNTRVLARAAVNMIRVRNMPPGPIA
                     ESILKLAQAVEALSRYLEDPEHPLETRGLALEAAGNATIVLHERNDLETNMLVGQIRA
                     TALDLLQASGMDYAAALQAVDEAALSSAGEQSVTVE"
     gene            complement(3570..4961)
                     /locus_tag="VFJ72_13755"
     CDS             complement(3570..4961)
                     /locus_tag="VFJ72_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET7480566.1"
                     /translation="MSITTTASTHELASFLAGIQSKDLPSALVERTEELFLDWAASAL
                     ASRGARPVVALERLAERMGPESGPSEVLVSRRRTSPLFAALVNAASSHFVEQDDLHNA
                     SVFHPGTVVFPAALATAQETGASGKDFLTAAVVGYEAGVRVGSYLGRSHYKVFHTTGT
                     AGTLAAAAAVSHLLGADEDTMLHALGSAGTQAAGLWEFLRDAADSKQLHAAKAASDGL
                     LAAYLARDGFTGARRILEGPQGMAAGMSSDADSSKLLEGMGERWAVLETSFKYHASCR
                     HTHPAADALLTAMRERGLKADDIRRVRARVHAAAIDVLGPVTDPRTIHQSKFSMGFVL
                     AQIAVRGSAGIDDFTEEALGDPELRAFHDRVEMVLDPEVDAAYPGRWIGLVDVETEDG
                     ETFTSRVDVPKGDPDNTLSRAEIEEKARRLAAYGGGVASKDLERLMSRSWSLGDAPDV
                     RDLLPPNVPPASR"
     gene            complement(4998..>6283)
                     /locus_tag="VFJ72_13760"
     CDS             complement(4998..>6283)
                     /locus_tag="VFJ72_13760"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET7480567.1"
                     /translation="SWVLEPVPLAATSVPIIFMLAATGAASPDKAVSGFGSSATLLLT
                     TGFMMAAAIEKTPLARRLTYRFLLRVPPSSSGVLAGLLGALVVLAVFVPSTVVRAVAL
                     LPVVVAVAQAFVRYGSPNGAKRLLLGLSFGATLGGIAILPAAVVNVLAVDLVAGAGGE
                     RITYFDWLALTWPVEIFAFLALWAGLVVFFPTRGERRLNQDTVAERLRDLGPVTGAEK
                     RLAGILVLTATLWCLEPLTGWHPSVPAFLAVALMTVSPLRVVEWNEVLGISWGSIFLF
                     GASLSLAFTLRDTGAAEWLGGQLLGELTTNLAHAGAVAGVLVIAAIMLVYQLAFAGST
                     PAAATLLPIMLAGSGSLQLPVGAVGVTVALSGLATFVLPSQAMANLVTYETGLYTVRD
                     LLKVGLLLTLVFLAILGVVAALYWEPIDFIGPDAR"
BASE COUNT         1215 a         2020 c         2011 g         1037 t
ORIGIN      
        1 gtacgcgaca gcaccttcga ccgatacgag ggggccgtcc ggctccacat caagccggcc
       61 ctgggcaggc taaagctcaa gaagctcacc cccgcccacg tccagagctt ctaccgggac
      121 aggctcgacg ccgggtgcgc ccaggcgtcc gtcaacaaga tgcacgtggt gctgcacaag
      181 gctctcaagc aggcggtcga gtggcacatg gtcccgcgca atgtcgccgg ggcggtcaag
      241 gccccgcggc cggcgcccag ggagatgcac ccactctcgg cggaggagac gcgccgcctc
      301 ctcgaagcgg cgcgcggtga cagactcgaa gcgctgtacg tgctggccgt ccacacgggg
      361 atgcgccagg gcgagctctt ggccctgaag tggcaggacg tggacctgga gaacgccacg
      421 atgagcgtgc ggcgcacggt cacgaagagc ggaggcaagt tgctgctggg cgagccgaag
      481 acgaagaaga gccggcgcac catccaactc accggggcgg cggtgcgggc gctgcgcggg
      541 cacctcgaca gacagatgaa ggagatcgaa cacctgggca acctctacag ggatgacggc
      601 ctggtcttca ccagcgaggt cggcaccatt atcaacccga ccaacctgcg gaagaggtct
      661 ttcgcgtcgc tcctgaagaa agcgaggctg cccgggatcc gcttccacga cctccggcat
      721 acctgcgcga cgttgctctt cggccgcaac gtccacccca agtacgtcca ggagctcctc
      781 ggacactcaa acgtggggat cacactagac acttacagcc acgtcatacc ggggatggga
      841 gatcacgccg ccagggcgat ggaggatgcg ctctccaggc cggtaccacg cccttcccgg
      901 ttagcgtcac cgccgatgga gtagaaggtc gcgttggtgt actactggtg tacaaagggc
      961 cggaggagat cccggccctt ctttatgtcc tcgcgtttct cctgcaaatc gacgtatttt
     1021 aagagtggag ccgaggggat tcgaacccct gacctccgcc gtgcaaaggc ggcgctctcc
     1081 caactgagct acggccccat tggataagag tctgagtcta cgcgcccgga gcgtgttctt
     1141 caatgcgcgg ggcattccgc agagggtaca atgtgtatcc agaaggacct tgactgagag
     1201 gtgggacaga tggctcttta cgaatacaag tgcgccgagt gtgaggagcg cttcgacctg
     1261 atgcgctcga tgagcgccgc cgatgagccg gcggagtgtc cggagtgcgg ctcggtggaa
     1321 tccaggcgcc tcatctcgaa ctttgcctcc ataacccccg gcgcctcggc cctcaacacc
     1381 aaccccatga tggacgcccg catcgcgagc ggcaacgccg ccggcggatg ctgcggtgga
     1441 ggctgcggct gccactaaag gtttgtccgc ggatactgag caggtcccgg acctcgtcct
     1501 caaggggata gaggagttta accggggcga gttcttcgag tgccacgagt atctggagga
     1561 agcctggatg caggaacctc gcagggtgcg gttcctttat cagggaatcc ttcaggtcgg
     1621 ggttggcttc taccatctca agaacggtaa ctggcgtgga gcgacagggc ttctgcgcaa
     1681 cggcacggcc cggctgagag agttcgagcc cgacgcgatg ggcatagacg tcgggaggct
     1741 tatccgggag agcgagcgat gcctggccga gttggaggag ctgggacgcg agcgggttgg
     1801 ggagtttgat ctctccgcca tacccagggt ggaatgggtc caggagcgtt agtgcccggt
     1861 ggttaggaca aagactttaa atcactcatc gcccagtata tagccggagc cgcgtcttta
     1921 ctcgaccgtg acggactgtt ctccggcgga gctaagagcg gcctcatcta cagcttgcag
     1981 cgcggccgcg tagtccatgc cggaggcctg caacagatcc agggccgtcg ctcttatctg
     2041 gccgacgagc atgttggttt cgaggtcgtt gcgctcgtgg agcacgatgg tcgcgttccc
     2101 ggcggcttcc agggcgagcc cgcgggtttc gagggggtgt tcgggatctt cgaggtacct
     2161 ggatagcgcc tcgaccgcct gggccagctt caggatgctc tcggcgatcg gtcccggcgg
     2221 catgttcctg acccgtatca tgtttacggc ggcgcgggcc aacaccctgg tattgcggat
     2281 cgcaaggtcg agttgatcgg cggctgcggc gtagaacccg agctggccga gcgcccgtct
     2341 cctggcggga gagagacgca ctgtcccgta tccattctcg agcgcctcct tgaagccgcc
     2401 cacccgagca tccatgtgac gcgccttcac cagagcctcc tccgcctgtt cgatgtccct
     2461 ggtgtagagc gcctcgtaga tctcctccaa cacgccgatg aactcatcga agatcggatg
     2521 cgccgcctga gcaacggtgt ggccggggtt gttcgggaga atcgcccgga tcgcgagcgc
     2581 gaggccgctg cccaccagcg cctctacgaa gcggtctccc gacgcgccgg agctcggaga
     2641 ggcgaggatc accatcagca acgccgaaac acccgcttcg gtgatggaga gcgctccacc
     2701 tccgaagaac gtgactatcg ccgtcgccgt gcccacgacg agggcgatct gcaacgctcc
     2761 cgagcccacc agggataata agaggtccgc caccgtgagc ccgcaggcta caccgaagac
     2821 caactccacc gcctgcctcc actctctccc gttcacgacg gagagagaga tcaacgccgc
     2881 tatcggagcg aaggccggct gattgtgccc gagcagagcc gaagcaaccc agtaggccac
     2941 gccagcggcc agcgccttct gggcgacctg acctccttca gaccacaggc gaccgaacgc
     3001 cagctcaaaa cgatgtagaa aaccgggcat caaaccgccc aacctcgacc gccctatcgt
     3061 ctccgcgcta atggtcggcc acctcctttc taatatcctg tccgcgaatc gctctttcat
     3121 ccgcatcagc aaacttactt cctggtatat gcaacgtgca tataccttcg gagtttactc
     3181 attcctcggg gataggcaag ctttctgtac gcgattcgac cggttgttat caaactgttg
     3241 ccaacagttt acaagcgtgt aacttggtag cagatggact cccatggacc atgtagtgcc
     3301 ggcaggtagt tagcgtcgct gatcttcgtt agcggcagac tgcccgcctc tacccgcgac
     3361 caacgaagct cataaatgct cccgagaata ggaggctcgt tcgggaagat atgacgaagc
     3421 gactccttat agagaagctg ccacacgggg cgcggggcgg ctctccaact cgcggacgaa
     3481 aaccatatga gagaaacccg ggggctgaga cgcgccttcc ttcggcgagc gggacttcca
     3541 ctcgccgggc atccgggtgg cggcttcgac tatctggacg ctggcgggac gttcgggggc
     3601 aacaggtccc gtacatccgg ggcgtctcca agactccacg agcgggacat caggcgttcc
     3661 agatccttgc tcgctacccc gccaccgtag gcggccagcc ttctcgcctt ctcttcgatc
     3721 tccgcccggc tcagggtgtt gtccgggtct cctttcggca cgtctacgcg cgaggtgaaa
     3781 gtctcgccgt cctccgtctc cacatccacc aagccgatcc atcgtccggg atacgccgcg
     3841 tccacctccg ggtccagcac catctccacc ctgtcatgga aggcccggag ttcggggtct
     3901 ccaagcgcct cctcggtgaa gtcgtcgata ccggcgctcc ccctgacggc gatctgggcg
     3961 agcacgaacc ccatcgagaa cttggattgg tggatggtcc gcgggtcagt aaccggcccg
     4021 agcacgtcta tcgccgcggc gtgtacccgg gcccgtaccc tgcgtatatc gtccgccttc
     4081 aggccgcgct cccgcatcgc cgtgagcagt gcgtcggcgg ccgggtgggt gtggcggcag
     4141 gaagcatggt acttgaagga ggtctccagg acggcccaac gctcgcccat accttcgagg
     4201 agcttcgacg agtccgcgtc cgaggacatc ccggcggcca tgccctgcgg cccttcgagg
     4261 atgcgccgcg ctccggtgaa cccatcgcgg gcgagatagg cggcgagcag tccgtccgag
     4321 gcggccttgg cggcgtgcag ttgcttggag tcggcggcgt cgcgcaggaa ctcccagagc
     4381 ccggcggcct gggttccggc ggagccgagc gcgtgaagca tcgtatcctc atcggcgccc
     4441 aaaaggtgcg agacggcggc cgcagccgcg agcgtgccgg cagtgcccgt ggtgtggaaa
     4501 accttgtagt gcgaacgtcc gaggtagctt ccgaccctca ccccggcttc gtagccgacc
     4561 actgcggccg tcaggaagtc cttgcccgag gccccggtct cctgggcggt cgccagagcg
     4621 gcggggaaga ccacggtacc cggatggaat acggatgcgt tgtgcagatc gtcctgctcg
     4681 acgaagtgcg aagaggccgc gttcaccagc gcggcgaaga gaggagaggt gcgccgtcgg
     4741 gagacgagca cctcggacgg tccgctctcc ggacccattc tctccgcgag gcgttccaga
     4801 gccactaccg gcctggcgcc tcggctcgcg agcgcggagg ccgcccagtc caggaaaagc
     4861 tcctccgtcc gctcgaccag cgcggatggc aggtctttgg actgaatgcc ggcaagaaaa
     4921 gacgccagtt cgtgagtgct agcggtagtc gtgatgctca tatctctcct tcgaagtccg
     4981 ttcttctgtt cgcgggatca ccgggcgtcc ggacctataa agtcgatggg ctcccagtag
     5041 agcgcggcga ccactcccag gatagccaga aacaccagtg ttagcagcag cccaaccttt
     5101 agaaggtcgc gtaccgtgta gagaccagtc tcatacgtga cgaggtttgc catcgcttgc
     5161 gagggcagga cgaaggtggc gagtcccgag agggctaccg ttactcccac cgctccgacc
     5221 ggaagctgca gtgacccgga gccggcgagc atgatgggca gaagcgtcgc tgctgcgggc
     5281 gtggagccgg cgaaggctag ttggtagacg agcattatcg cggctatcac cagcacgcct
     5341 gccacggctc ccgcgtgcgc gaggttggta gtcaactctc cgagcagttg tccgccgagc
     5401 cattccgcgg ctcccgtatc tcgcagggtg aacgcgagcg agagacttgc gccgaacagg
     5461 aagatggaac cccaggagat cccgagtacc tcgttccatt caacgacccg gagaggtgag
     5521 accgtcatga gcgcgaccgc caggaacgcc ggcaccgaag gatgccagcc ggtgagaggc
     5581 tccagacacc agagcgtcgc ggttagcacg agtatgcccg cgagacgttt ctccgctccc
     5641 gtaacaggtc cgaggtctcg aagacgttcg gcaacggtat cctggttcag ccgccgctct
     5701 ccgcgcgtcg ggaaaaagac gaccagaccc gcccagagcg ccaggaaagc gaagatctcc
     5761 accggccacg tcagcgcgag ccagtcgaag tacgtgatgc gctcgccgcc agcaccggca
     5821 accaagtcta cggccagcac gttgacgacg gcggcgggca ggatcgctat tcctccgagc
     5881 gtcgccccga aggacagacc gagcaagaga cgtttcgccc cattcggcga cccgtagcgg
     5941 acgaacgcct gcgccaccgc gacgacgacc ggaaggagcg ccacggcccg aaccaccgtc
     6001 gaggggacga acacggcgag caccacgagc gcgccaagta gacccgccag cacgccggac
     6061 gaagaaggcg gaacgcgcag caggaaacgg taggtcagcc tgcgggcaag aggggtcttc
     6121 tcgatggccg ccgccatcat aaagccggtc gtgagcagca acgtcgccga cgacccgaac
     6181 ccggagaccg ccttgtctgg agacgccgct cccgtcgccg cgagcatgaa gataatgggg
     6241 actgaagtcg cggcgagcgg gaccggctcc agcacccagg agg
//
LOCUS       DASSMB010000238        13693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_454648, whole genome shotgun sequence.
ACCESSION   DASSMB010000238 DASSMB010000000
VERSION     DASSMB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454648"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..941)
                     /locus_tag="VFJ72_13765"
     CDS             complement(210..941)
                     /locus_tag="VFJ72_13765"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480568.1"
                     /translation="MEQQKPVALWCVPRSISTAFERVFVERDDFEVFHEPFSASYYHS
                     EDRLSERFADDPPQPEHNYDRVVERIFEPRDQRVFLKDMAYQAKPLLNREFASRFVNT
                     FIVRDPKYVLTSMYKMWPDFTFEEAGYEELYRLFRICEEAGQEPVVVDAMTFSENPVE
                     VLSVYCERVGIPFREDDLTWQPREVRRWENWEGWHDVAQRSSGIKRAERRDPVLPREL
                     EEMYERCLPAYYDLAAHAIPGTRHR"
     gene            complement(958..2343)
                     /locus_tag="VFJ72_13770"
     CDS             complement(958..2343)
                     /locus_tag="VFJ72_13770"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET7480569.1"
                     /translation="MSQTVTMEKLVAFCKRRGFVFQSSEIYGGFRSSYDYGPLGVEMK
                     RNIKEEWWRRTVHMRDDVVGVDAAIIMHPKVWEASGHTATFNDMLVESRTSGRRYRAD
                     HLIEQATGRDDVEGLSAEELTEIIQSDDRIKDPMDGGRDFAPVRPFNLMFETYTGPVK
                     GPENIAYLRPETAQGIFVNFKNVMQTSRVKVPFGIAQQGKSFRNEITPGNFIFRTREF
                     EQMEMEFFVKPGTDEEWHEFWIEERWNWYISLGLNPENLRRYEHPKEKLSHYSKRTVD
                     IEYNFPFAGWSELEGIANRTDFDLKQHAQFSGENLEYFDQATGERYTPYVIEPAGGPD
                     RIMLAFLSDAYTEEEVNGEERTVLKLHPRIAPIKAAVFPLSKKEPVSTVARDLYDDLK
                     GDYRIFYDDSGSIGRRYRRQDEAGTPFCVTVDFDTMDDKSVTIRDRDTMQQERIPIQA
                     VRERLQKLISG"
     gene            2457..3041
                     /locus_tag="VFJ72_13775"
     CDS             2457..3041
                     /locus_tag="VFJ72_13775"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET7480570.1"
                     /translation="MRAVETFLAIGIGVGLASVAGVRAFLPPALASLFALFGLFYVPE
                     QVVGIGPEILLGGLAALAVLEIVLDKVRALERGFNMAMVPVRMASGAVLFAAAMGLDV
                     DMGSLVWLGAGAVIAGVVAVLKLVLRPALGNSAGVSGSFLSLIEDAVALVGSAAGCFV
                     PLIPLLLVAFLLFFFYRIRKRRGRRYGGLRILGD"
     gene            3071..4015
                     /locus_tag="VFJ72_13780"
     CDS             3071..4015
                     /locus_tag="VFJ72_13780"
                     /inference="COORDINATES: protein motif:HMM:NF003259.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HET7480571.1"
                     /translation="MDQREITIVKGNRETPFSRGVLAQTLAQAGARQELAHRVASEIR
                     SELLAEERYTVEEEEVLARVRSKLMTTDAIIVTRLDKWRILRESSEPVVVLIGGATGV
                     GTSTLAADVARRLNIQSVIGTDSIREVLRRAISPDLLPLLHKSSYEITARDIRVPVDE
                     NETVLFGFRQQASQVAVGVEAIVDRGLKEGTNIIVEGVHLVPEIILDRYRDHPNVCTL
                     VVYLSDQDAHRSRFYIRALGTAMRRPAEEYIAHFKEIRQIHDYILESAARTDVQTVEN
                     LSIENTSDAAVEIVANRVSGIAETARRRSASLLLDANT"
     gene            4082..4579
                     /locus_tag="VFJ72_13785"
     CDS             4082..4579
                     /locus_tag="VFJ72_13785"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7480572.1"
                     /translation="MIDKERLRTFILGGVVGALAGILLAPRSGREIRGTVANRAGEAR
                     ERSRERYFEAQERMQERFSESREGIRRRPDPAREPTVGGGSPEAPGKTYGAPGRPPLR
                     DVSRGVVDEPMREPTAEPVDGEEAGADSDARSEELRRKVRETRARLRGRLQANEEGGE
                     EPDDI"
     gene            4589..5266
                     /locus_tag="VFJ72_13790"
     CDS             4589..5266
                     /locus_tag="VFJ72_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480573.1"
                     /translation="MKHISFVPLVEEISPEEAGKTPEIRAVVSYWTEGSSDAAEWFEE
                     LNGEFGTVGLMMRRGEELLGYLVYAPRRYLPRAGRYPLGPLDGETVLLAYVAGEQRAR
                     RHLLVRMLRDLRYRNVGKVEAVASDIGLPGHVSTRFLIESGWRPVRGCLYRGLPYTLA
                     RTDLASVVEVGDLARGLLGRVKLPNLKGPYPLPGAFVRTVEEPEPARAEENPHLGGPL
                     RPAGSRS"
     gene            complement(5241..5732)
                     /locus_tag="VFJ72_13795"
     CDS             complement(5241..5732)
                     /locus_tag="VFJ72_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480574.1"
                     /translation="MKGVILWLSGAALAFLLTSGAFLLLANASGGPSEKSLRPDSPSA
                     ESGPALDLQLGRDYLSSLKQRPDQKLSIGVHNAGSQKLSDINVSVKVYSENTALSDVR
                     YYRSSIKDLAAGETVTADFTLDLSFAGQNTDQGAEPARAIIEVRAATPSGVSSVRTAI
                     LPA"
     gene            complement(5729..6925)
                     /locus_tag="VFJ72_13800"
     CDS             complement(5729..6925)
                     /locus_tag="VFJ72_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480575.1"
                     /translation="MRCRVLARALARVLTPLLILAAFATGCGNTARSVPISPLPTGQD
                     QTDFGLGPGVKPLSFGPGDKSAPAWSPDDERVAFVVDGYVVDKPLDAQDLRRWTTKDF
                     GAERAEWRSEDSLTIFAREPSSEEAPGTVYRTLPKDTPFAVKQIAADVLTISAGPGDE
                     GLVVALETNQYESGLARIGADGRMDQVYTPLVGGRVTGLSISPDGRRIAIAARGAATF
                     ALHVLDLTSGSLRTIARLNPGMEIHGGPEWTDQGIYYIAGEEDTGDRSVVPHHLYRVP
                     PSSTTPEPAPGVGEDFVASSLRASPDGKQLAIVGRRNPTSSTNLYILDLATDNLKAVT
                     ANEDMEIKIGSDDLAWSNSGDRVAIVARGVISGPRVLSAPADTLLTDFYNLYEVPVTA
                     SRKSAS"
     gene            complement(6915..8711)
                     /locus_tag="VFJ72_13805"
     CDS             complement(6915..8711)
                     /locus_tag="VFJ72_13805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7480576.1"
                     /translation="MTSLVIGLVLYYFAEQRLVNAESDLLVQRSRTANAGASDFLEGL
                     RDPDSQDLPTPDTYANELVRSVSDPTGLGVLYVGPEGKPLAARDGLGNSIPPSEDYKR
                     LELDKGIIQEAMDSPQRQGRLVTSGGLRRIAAWPLTGADGRVMGVMVYNATGDELENT
                     LAYLRYGILGAILTSVALAALASYLLTRQITRPLSETRDAAIRVASGDYAATVPVTSD
                     DELGEVARSFNYMAEEIEHYVGEIQEQKSRLEAVLEASPEAVIATDAAERITMANPAA
                     ARMLGIRPNDSGRTLEEIGSPEGIMLCLREAAATGVAVREVESNGKTYWTYAARMNRE
                     DAPGAGNGRGAEAGTILAVRDITEHRSLEKAKTAFVSDVSHELRTPLTTIQSAVSLLE
                     RAQERLDPLEHRALQLADQELQRIRGMVEELLTLAQMDSWQYALELNPSDLAIIVQNA
                     VEALENRAERFGIEIVFHDGIGHPCVCDSEKLYQVFLNLLDNAIKYSDPGGHVGITIE
                     EDASDLIVRVRDKGVGIPEEDLEQLFERFYRVHKDRSRATGGSGLGLAISKQIVEMHG
                     GEISVQSEVGEGSTFTVRLPRAPLPRSANYAM"
     gene            complement(8820..9512)
                     /locus_tag="VFJ72_13810"
     CDS             complement(8820..9512)
                     /locus_tag="VFJ72_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480577.1"
                     /translation="MEETILLVDDDAALLEVTSIVLASEGYRVITAEDGIEALQVVGR
                     DNLDLVVLDIMLPRMSGFEVLKKIREQSDVPVILLTAKSQSVDKVVGLELGADDYITK
                     PFDTKEVLARIKAILRRFGRQEGKSRNGTLRLGPLELDPEGYTVTRDGELLDLTPTEF
                     KILALLLRRPGQAFTRGQISEAVSTSQHYLASRYIDVHISRLRGKIERDPSRPEIIQT
                     VPSVGYRAARPA"
     gene            9698..11605
                     /gene="dnaK"
                     /locus_tag="VFJ72_13815"
     CDS             9698..11605
                     /gene="dnaK"
                     /locus_tag="VFJ72_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET7480578.1"
                     /translation="MGKSIGIDLGTTNSCVAVLEGGDPIVITNSEGERTTPSVVAFDR
                     KSGERLVGQLARRQAVTNPERTIYSIKRFMGRRYSDVKTEAERVGYTVKSGNGGDIRV
                     QVDDKEYSPPEISAMTLQKLKRDAEEYLGEEITDAVITVPAYFEDAQRQATKDAGRIA
                     GLNVKRIINEPTAAALAYGLDKEQEQTILVFDLGGGTFDVSILELGEGVFEVKATSGN
                     NHLGGDDFDAKVVEWLTSEFKKAEGIDLSKDKMATQRLVEAAEKAKRELSSTTSTSIN
                     LPFITADQNGPKHLDLTLTRAQFNNLTADLVEATAGPVRQAMSDAGLQSGQVDQVILV
                     GGSTRIPAVQDKVKEITGKEPHKGINPDEVVAIGAAIQAGVLAGEVKDVLLLDVTPLS
                     LGIETKGGVFTKLIERNTTIPTRKAETFTTADDNQNSVEIKVYQGEREIAAYNKLIGN
                     FQLVGLPPAPRGVPQIEVSFDIDANGIVNVGAKDLGTGKEQRITITASSGLSDTDIEQ
                     MVRDAESHADEDRRRREEADVRNSADNLIYTVERSLKDVDGKVDGATKLEIENAIKEA
                     REALAGNDVEEIKAKQEALMTASHKLSETLYQQAQQQQEAASSSGTENPDEDLVEDAE
                     VIDEDDEEQRK"
     gene            11676..12335
                     /locus_tag="VFJ72_13820"
     CDS             11676..12335
                     /locus_tag="VFJ72_13820"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7480579.1"
                     /translation="MSNEERKQGSPEEEIQPGDQPVEEGQELPEVAAEEPKADNSAAA
                     TQEEPAETGEDAPQDEPDSDAGTLEEVTALSEKLEITSRERDDYLDALRRLKAEFENS
                     RKRQERERQRILQTASERLITELLPVLDNLDRALDHEGDIREGVRATRDQLADVLGRE
                     GLLPVASDGQSFDPNVHEAVMSQPSEDHEEGTIIQTFERGYVLNGKPIRAAKVVVASQ
                     G"
     gene            12338..13453
                     /gene="dnaJ"
                     /locus_tag="VFJ72_13825"
     CDS             12338..13453
                     /gene="dnaJ"
                     /locus_tag="VFJ72_13825"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7480580.1"
                     /translation="MQTKDLYKVLGVEKEASQDDIRRAYRKLARKHHPDANRDDPKAE
                     DRFKEIQSAYEILSDPNKRREYDEGPRTFFGQGAGRPGARGAGTTGASQADFSDLFGT
                     FGDIFGAGASARSQPAATRGNNITVNVNLKFKDALNGVTTRVAVPVEEVCGDCRGTGA
                     APGTMPRTCPDCGGLGTQSRDQGFFALSTSCSRCGGEGTIVEKPCPRCAGQGRVKKTR
                     QVTVKIPAGARDGMKIRVPGRGSAGQKGGPAGDLYVVTRVEEHPVFKRRGDDFVVEVP
                     VSFAEAALGAEIRVPKPDGGTVKLRLPAGTQEGRQFKVRGAGAPRPKSGGQGDLIVRA
                     KIVVPKKLNRRERDILEALAEERDEDVREDLFRRVGS"
     gene            13450..>13693
                     /locus_tag="VFJ72_13830"
     CDS             13450..>13693
                     /locus_tag="VFJ72_13830"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7480581.1"
                     /translation="MSFRKRPVFMIGVAAELIGVHPQTLRMYEQKGLLRPRKSIKNTR
                     LYSQEDVELGRYIQKLTQEMGMNLAGVGKVLDLERKL"
BASE COUNT         2472 a         4128 c         4628 g         2465 t
ORIGIN      
        1 acgtcgtcgc gatagtcatg tacgtggcct tccgtctcgt ccggcgcgcc cagggcatgg
       61 acctcaagat ggtgtacgac gagatcccgg aggagtagcg agcgggtatc cgctctcagc
      121 aatcggtccg agaaatgtag accgctgatt acacaagaga ggaccccccg cttctcaaag
      181 gcgggggtcc tctttcgtct attggtagtt tacctgtggc gtgtgccggg gatagcgtgc
      241 gccgcgaggt catagtaggc gggcagacac ctctcataca tctcttcaag ctccctgggt
      301 aacacggggt cccgcctctc ggcacgcttt atgccgctac tcctctgcgc cacatcgtgc
      361 catccctccc agttctccca gcgccttacc tccctcggct gccaggtgag atcgtcctcc
      421 cggaacggta tcccaacccg ctcgcagtac acggataaga cctctaccgg gttctcggag
      481 aacgtcatcg catcaaccac gacgggctcc tgaccggcct cctcgcagat cctgaacaga
      541 cgatacagct cctcgtagcc cgcttcctcg aaggtgaagt cgggccacat cttgtacatg
      601 gaggtaagga cgtacttcgg atcgcgcacg atgaaggtgt tgacgaagcg tgaggcgaac
      661 tcgcggttga ggagaggctt tgcctggtag gccatgtcct tgaggaagac ccgctggtct
      721 cggggctcga agatcctctc gaccacccgg tcgtagttgt gctccggctg gggcgggtca
      781 tccgcgaacc tctcggagag gcgatcctct gaatggtagt acgaggcaga gaagggttcg
      841 tggaagacct cgaagtcgtc gcgttcgaca aagacccgct cgaaagctgt cgagatcgaa
      901 cgcggtacgc accacagcgc cacgggtttt tgttgctcca cggagagcct ctttcttcta
      961 tcccgagatg agcttctgca gccgctcccg aaccgcctgt atcgggatgc gctcctgctg
     1021 catcgtgtcc cggtcacgga ttgttacgct cttgtcgtcc atggtgtcga agtctaccgt
     1081 gacgcagaag ggcgtccccg cttcgtcctg gcggcggtag cggcggccta tagagccgga
     1141 gtcgtcgtag aagatgcggt agtcgccctt caggtcgtcg tagaggtccc tggcgaccgt
     1201 cgagacgggc tccttcttgg agagcgggaa gacggcggcc tttatgggcg cgatgcgcgg
     1261 gtggagcttc aggacggtgc gttcctcgcc gttcacctct tcctccgtgt aggcgtccga
     1321 gaggaaggcg agcatgatcc ggtcgggtcc gcccgccggc tctatgacgt agggggtgta
     1381 gcgctcgccg gtcgcctggt cgaagtattc caggttctcg ccggagaact gcgcgtgctg
     1441 cttcaggtcg aagtccgtgc ggtttgcgat gccttcaagc tccgaccagc cggcgaacgg
     1501 gaagttgtac tcgatgtcca cggtgcgttt cgagtagtgg gagagcttct ccttcgggtg
     1561 ctcgtagcgg cggaggttct ccgggttcag gccgagggag atgtaccagt tccagcgttc
     1621 ctcgatccag aactcgtgcc actcctcgtc cgtaccgggc ttcacgaaga actccatctc
     1681 catctgctcg aactcgcggg tgcggaagat gaagttccct ggcgtgatct cgttccggaa
     1741 agacttgccc tgctgcgcta tcccgaacgg caccttcacc cggctcgtct gcatcacgtt
     1801 cttgaagttc acgaagatgc cctgcgccgt ctccggacgc aggtacgcta tgttctccgg
     1861 tcccttgacc ggcccggtgt acgtctcgaa catcaggtta aaggggcgta cgggcgcgaa
     1921 gtcccggccg ccgtccatcg ggtcctttat ccggtcgtcg gactggatga tctccgtcag
     1981 ttcctcggcg gagagacctt ccacgtcgtc gcggccggtc gcctgttcga tgaggtggtc
     2041 cgcccggtag cggcgtccgc tggtgcggct ctcgacgagc atgtcgttga aggtcgccgt
     2101 gtggccgctg gcctcccaca ccttcgggtg catgatgatg gcggcgtcca ctccgactac
     2161 gtcgtcgcgc atgtggactg tgcggcgcca ccattcctcc ttgatgttgc gcttcatctc
     2221 gacgcctaaa ggaccgtaat cgtaagagga gcggaagccg ccgtagatct cggagctctg
     2281 gaacacgaag cctcggcgct tgcagaaggc gactaacttt tccatcgtta cggtctgcga
     2341 cacgggaccc tccgaactgt tagatgcaag acacttgtaa gagtccggca agtatagctc
     2401 agggggtgga actgtttgcg gatggagaga gcttgttgcg gagcacaatg gttataatgc
     2461 gcgctgtgga aacctttctc gccataggaa tcggcgtcgg actcgcctcg gtagccgggg
     2521 tccgggcttt cttgccgccc gctctggcct ctctctttgc gctattcggc ctcttctatg
     2581 tccccgagca ggtggtcggg ataggaccgg aaatccttct cggcgggctg gcggcgctgg
     2641 ctgttctgga gatagtcctc gacaaggtcc gggcgctgga gcgcggtttc aacatggcga
     2701 tggtgccggt ccggatggca tcgggggcgg tgctgttcgc cgcggcgatg ggcctggatg
     2761 tagatatggg ctccctggtg tggctgggtg ccggggccgt gatcgccggg gtggtggcgg
     2821 tgctcaagct ggtgttgcgt cccgcgctgg ggaactcggc gggtgtctca ggtagcttcc
     2881 tgagcctgat cgaggacgcg gtggcgctcg tgggttcggc ggccgggtgc ttcgtgccac
     2941 tcattccttt gctcctggta gcattcctgc tattcttctt ctaccgcatc aggaaacggc
     3001 gcggtagaag gtacggaggc ctccgcattc tcggggatta ggggctccgg cgctcttttg
     3061 gaggggtgct atggaccagc gggagatcac gatagtcaag ggcaaccggg agaccccgtt
     3121 ttcgcgcggt gtcctggcgc agactctcgc tcaggccggg gcgcgtcagg aactggccca
     3181 tcgcgtagcc agcgagatcc gctcggagct tctggccgag gagcggtaca cggtcgagga
     3241 ggaggaggtc ctcgcgcggg tgcgctcgaa gctgatgacg accgatgcta tcatcgtgac
     3301 ccggctggac aagtggcgaa tcctgcgcga gtcctcggag ccggtggtag tgcttatcgg
     3361 aggggcgacg ggggtcggga cgagcacgct ggccgcggac gtggcgcggc ggctgaacat
     3421 ccagagcgtc atcgggacgg actccatccg ggaggtactg cggcgggcca taagcccgga
     3481 cctgctgccg ctcctgcaca agagttccta cgagatcacc gcgcgggaca ttcgtgtgcc
     3541 ggtggacgag aacgagacgg tactcttcgg tttccggcag caggcgtcgc aggtggcggt
     3601 gggcgtggag gccattgtgg accgggggct caaggaaggg acgaacatca tcgtcgaggg
     3661 tgtgcatctg gtgccggaga tcatacttga ccgctaccgg gaccacccga acgtctgcac
     3721 gctggtggtt tacctctccg accaggacgc gcaccgctcg cgcttctaca tccgggctct
     3781 gggtacggcg atgcgccgtc cggccgagga gtacatcgcg cacttcaagg agatacgcca
     3841 gatccacgac tacatcctgg agagcgcggc gcgcacggac gtgcagacgg tggagaacct
     3901 ctccatagag aacacctccg acgccgccgt ggagatcgtg gcgaaccggg tctccgggat
     3961 cgccgagacg gcccgccgcc gatccgcctc tctgcttctc gacgcgaaca cctgaccccg
     4021 gacccgatac tccggccgca aaggggttag aatacgcgca gatcccagga ggtagacggc
     4081 gttgatcgat aaggagcggc tgaggacttt tatcctcggt ggagtggtag gggctctggc
     4141 gggtatcctt ctcgcgccgc gcagcggccg ggagatcagg gggacggtgg cgaaccgcgc
     4201 cggagaggcg cgcgagcgga gccgcgagag gtacttcgag gcgcaggagc ggatgcagga
     4261 acgtttctcc gaatcccgcg agggcatccg gcgccgcccg gacccggcgc gcgagccaac
     4321 cgtgggcgga ggctcgccgg aggctcccgg aaagacttat ggagcgccgg gcaggccgcc
     4381 ccttagggat gtctcccgcg gtgtggtgga tgagccgatg agggagccga cagcggagcc
     4441 ggtggacggc gaggaagcgg gcgcagactc cgacgcaagg tccgaggagc ttcggcggaa
     4501 ggtgcgcgag acgcgggccc gtctacgggg ccgcttacag gcgaatgaag agggtggcga
     4561 agagccggat gatatctagg agccgccgat gaagcacata tcattcgtgc cgctggtcga
     4621 ggagatctcg ccggaagagg cggggaagac gccggagatc cgggcggtcg tatcttactg
     4681 gacggaaggc tccagtgacg ccgccgaatg gttcgaggag ttgaacggcg agttcgggac
     4741 cgtgggactc atgatgcggc gcggcgagga acttctcggg tatctggtct acgctccgag
     4801 gaggtatctg ccgcgagccg gccgttaccc gctcgggccc ctggatgggg agaccgtcct
     4861 tctggcgtac gtggcgggcg agcaacgcgc gcgccggcac ctgctagtgc gtatgctccg
     4921 cgacctgcgc taccgcaatg tcgggaaggt cgaggcggtc gccagcgata tcgggctgcc
     4981 cggccacgtg tctaccaggt tcctgatcga gagcggctgg cgtccggtac gaggatgcct
     5041 gtaccgcggt ctgccctaca ccctggcgcg caccgatctg gcgagcgtcg tcgaagtagg
     5101 ggacctggcg cggggtctgc tcggtagggt gaagctgccg aacctgaagg gaccgtatcc
     5161 gttgcccgga gcattcgtcc ggacggtaga ggagccggag cccgcgaggg cggaagagaa
     5221 cccgcacctc ggaggacctc tcaggccggc aggatcgcgg tcctgacaga ggagactccc
     5281 gaaggggtcg cggcccggac ctcgatgatc gcgcgcgccg gttccgcgcc ctgatccgta
     5341 ttctgtcccg cgaacgacag gtcgagcgtg aagtccgcgg tgaccgtctc tccggcggcg
     5401 agatctttga tggaagaacg gtagtagcga acgtcggaga gcgcggtgtt ctcggaatac
     5461 acttttaccg agacgtttat gtccgagagc ttctgactac ccgcattgtg gactccgatg
     5521 gataactttt ggtctggccg ctgtttaagc gaggagagat agtctctccc gagttggagg
     5581 tccagggcgg gaccggactc cgccgaaggc gagtcgggcc gcaggctctt ctctgacggg
     5641 ccgccgcttg cgttggcgag gaggaggaag gctccggacg tcagcaagaa ggccagagcc
     5701 gctccggaga gccacaggat cacccccttc acgacgcgct ctttctggag gcggtgacag
     5761 ggacttcgta gaggttgtag aagtccgtga gcagggtatc ggccggggcg gagagcaccc
     5821 tggggccgga gatcacaccg cgggcgacta tcgccacgcg gtcgccggag ttcgaccagg
     5881 ccaggtcgtc ggacccgatc tttatctcca tgtcctcgtt tgccgtgacg gccttcagat
     5941 tgtccgtcgc gaggtccagg atgtagaggt tcgtcgagga ggtggggttg cggcggccga
     6001 cgatggcgag ttgcttccca tccggggagg cccgcaggct cgacgcgacg aagtcctctc
     6061 cgacgcccgg cgcgggttcg ggggtggtgg aggatggcgg aacgcgatag agatggtggg
     6121 ggaccacgct tctatctccg gtgtcctctt cgccggctat gtagtagata ccctggtccg
     6181 tccactcggg accgccgtgg atctccattc cggggttcag gcgggctatg gtcctcaggc
     6241 ttccgctcgt gaggtcgagg acgtggaggg cgaaggtagc ggctccgcgc gcggcgatag
     6301 cgatccggcg gccgtccgga gagatggaga gcccggttac gcgacccccg acgaggggag
     6361 tgtagacctg atccatcctc ccatccgcgc cgattcgcgc gaggccgctc tcgtactgat
     6421 tggtctcaag cgccactacc agaccttcat cgcccggtcc ggcgctgatc gtcaggacgt
     6481 cggcggctat ctgctttacg gcgaacggcg tgtctttcgg gagggttcgg tagacggtgc
     6541 cgggcgcttc ttccgacgac ggttccctgg cgaagatcgt caggctatct tcggacctcc
     6601 actcggccct ttcggctccg aagtctttcg tggtccagcg gcgcaggtcc tgggcgtcga
     6661 gtggcttgtc cacgacgtag ccgtccacga cgaacgccac gcgctcgtca tccgggctcc
     6721 aggcgggggc gctcttgtcg ccgggaccga agcttagagg cttgactccg gggcccagcc
     6781 cgaaatccgt ctggtcctgg ccggtgggga gcggcgagat cgggacgctc cgggccgtgt
     6841 tcccgcagcc ggtggcaaat gccgccagga tcaggagagg cgtgaggact ctagcgaggg
     6901 ctctggcgag gactctacat cgcatagttg gcggagcggg ggaggggcgc tctgggtagc
     6961 cggaccgtga acgtcgagcc ttcgccgacc tcgctctgga cggagatctc accaccgtgc
     7021 atctcgacta tctgcttgct gatggcgagc ccgagcccgc tgccgccggt ggcgcgggag
     7081 cggtccttgt ggacgcggta gaaacgctcg aagagctgtt cgaggtcctc ctccgggatg
     7141 ccgacgccct tgtcccggac cctgacgatg aggtccgagg cgtcttcctc tatcgtgata
     7201 ccgacgtgcc cgccggggtc ggagtacttg attgcgttgt ccagcaggtt caagaagacc
     7261 tggtagagct tctcagagtc gcagacgcac gggtgcccga tgccgtcgtg gaagacgatc
     7321 tcgatcccga accgctcggc tctgttctcc agagcctcca cggcgttctg cacgataatg
     7381 gcgaggtccg aagggttcaa ctccagggcg tactgccagg aatccatctg ggcgagcgtc
     7441 aacaactcct ccaccatccc ccggatgcgt tgcaactcct gatctgcgag ctgcaacgcc
     7501 ctgtgctcca ggggatcaag cctctcctgt gcccgctcca gcaggcttac ggcgctctgg
     7561 atggtggtga gcggcgtcct cagctcgtgt gagacatcgg atacgaaggc agtcttggct
     7621 ttctccagcg atcggtgctc ggttatgtcc cggacggcga ggatagtgcc cgcctcggct
     7681 ccccgtccgt ttccggctcc gggcgcgtct tcccggttca tccgggcggc gtaagtccag
     7741 taggtcttcc cgtttgactc gacctctcgg acggcgacgc cggtcgcggc ggcctcgcgc
     7801 aggcacagca tgatcccctc cggagagcct atctcctcca gcgtgcggcc ggaatcgttg
     7861 gggcggatgc cgagcatccg ggctgcggcc gggttagcca tcgtgatccg ctccgcggcg
     7921 tcggtcgcta tgaccgcctc gggcgaggct tcgagtacgg cctccagacg ggatttctgc
     7981 tcctggatct ccccgacgta atgctctatc tcctcggcca tgtagttgaa cgaccgggcc
     8041 acctcgccca gctcgtcgtc gctcgtgacc ggcacggtcg ccgcgtagtc accggaggcg
     8101 acccggatcg ccgcgtccct agtctcggag agggggcgtg tgatctgccg cgtcagcaga
     8161 tagctcgcga gggcggccag cgccacgctg gtcaggatcg ccccgagtat cccgtagcgc
     8221 aggtacgcca gggtgttctc caactcatcc ccggtcgcgt tatagaccat tacgcccatg
     8281 acgcgcccgt ccgcgccggt gagcggccag gcggctatgc ggcgcagtcc gccggatgtt
     8341 accagccggc cttgcctctg cggagagtcc atcgcttcct ggatgatgcc tttgtcaagc
     8401 tccagacgtt tgtaatcttc ggacggcggg atggagttgc ccagtccatc gcgggcggcg
     8461 aggggtttgc cttccggtcc gacatagagc accccgagtc cggtcgggtc cgagacggag
     8521 cggactagct cattggcata cgtatcgggc gtggggaggt cctggctgtc cgggtcgcgc
     8581 agaccttcga ggaagtcgct cgcccccgca ttcgccgtcc gggagcgctg gacgagcagg
     8641 tcgctctccg cgttcaccag ccgctgctcg gcgaagtagt agaggacgag ccctattacc
     8701 aggctcgtca ccgacgatat gccgacgagc gcgaggaaga tcctggccct gaagctcagg
     8761 ccctgcagta tctccctttt gcccttcttc tctacgatct ctcctccggc cagggaccgc
     8821 taggccggac gggcggcgcg gtatccgacg ctcgggacgg tctggattat ctccggcctt
     8881 gaggggtcgc gttcgatctt gccgcgcagc ctggatatgt gtacgtctat gtaacggctc
     8941 gccagatagt gctggctcgt ggagacggcc tccgagatct gaccccgcgt aaacgcctga
     9001 cccggcctcc tcaacagcag cgccagtatc ttgaactccg tcggcgtcag gtccagcaac
     9061 tccccatcgc gggtgacggt gtacccctcc gggtccaact ccagcggccc gagacggagc
     9121 gtgccgttgc gcgacttgcc ctcctgccgc ccgaaacgcc gcagtatcgc ctttattcgc
     9181 gccaggactt ccttggtgtc gaacggcttg gtgatgtagt cgtccgcccc gagctccagt
     9241 cccaccacct tgtcaaccga ctggctcttg gccgtcagca ggatcactgg tacgtcgctc
     9301 tgctcccgga tcttcttcaa cacctcgaac ccgctcatcc tcggcaacat gatgtccaga
     9361 accaccaggt ccaggttgtc ccgtccaacg acctgcaggg cctcgatccc atcctcagcc
     9421 gtaatcaccc ggtacccctc ggaggcgagc acgatggaag tgacctccag caaagccgcg
     9481 tcatcatcca ccagcaaaat agtctcttcc aacccccgaa aacctccctc tgaaaattct
     9541 cttctcgaac cttaatccta cctcatgcca cgcctctaaa gaaacgccaa gagatccttc
     9601 gctcccacag caacttcatc aataaaactt gcaagtccta gacttagatt ttatactcta
     9661 ggcagtttag aaaacgacgt actttggagg agcctggatg ggcaagagca ttggcataga
     9721 cctcgggacg acgaacagtt gcgtcgcggt gctggaaggt ggagacccga tagttataac
     9781 gaactccgag ggggagcgca cgacgccctc cgtcgtggcg ttcgacagga agagcgggga
     9841 gcggctagtg gggcagctag cccggcgtca ggccgttacg aacccggagc gcaccatcta
     9901 ctcgatcaag cgcttcatgg gccggcgtta tagtgatgtc aagaccgagg ccgagcgtgt
     9961 tggttacacc gtcaagtccg ggaacggggg cgacatccgg gtgcaggtgg acgacaagga
    10021 gtactcgccg ccggagatct ccgccatgac cctccagaag ctcaagcggg acgccgagga
    10081 gtacctgggc gaggagatca ccgacgcggt cattaccgta ccggcgtact tcgaggacgc
    10141 ccagcgccag gcgacgaagg acgcgggccg gatcgcgggg ctaaacgtca agcgcatcat
    10201 caacgagcct actgccgcgg cgctggctta cggactggac aaggagcagg aacagaccat
    10261 cctcgtcttc gacctcggcg gcgggacgtt cgacgtctcg atcctggagc tcggtgaggg
    10321 tgtctttgag gtcaaggcga cgagcggcaa caaccacctc ggtggcgacg acttcgacgc
    10381 gaaggtggtc gagtggttga cctccgagtt caagaaggcc gaggggatag acctctcgaa
    10441 ggacaagatg gcgacccagc gtctcgtcga ggccgccgag aaggccaagc gggagctgtc
    10501 atccacgacg agtacctcca tcaacctgcc gttcatcacc gctgaccaga acggcccaaa
    10561 gcacctcgac ctcacgctca cgcgcgcgca gttcaacaac ctgaccgcgg acctcgtgga
    10621 ggccacggcc ggtccggtgc gccaggcgat gagcgacgcc gggcttcaga gcgggcaggt
    10681 ggatcaggtc atcctagtcg gcggctcgac ccgtataccc gcggttcagg ataaggtcaa
    10741 ggagatcacc ggtaaggagc cccacaaggg catcaacccg gatgaggtcg tcgccatcgg
    10801 cgccgctatc caggccggcg ttctggcggg cgaggtcaag gacgtgttgc tcctcgacgt
    10861 tacgccgctc tccctcggca tcgagaccaa gggcggcgtg ttcaccaagc tgatcgagcg
    10921 taacaccact atacctacac gtaaggctga gactttcacg accgcggatg acaaccagaa
    10981 cagcgtggag atcaaggttt atcagggaga gcgcgagata gctgcctaca acaagctgat
    11041 cggcaacttt cagctcgtag gtctgccgcc cgcgcccaga ggtgtacctc agatcgaggt
    11101 ttcgttcgac atcgacgcga acggcatagt gaacgtgggg gcgaaggacc tggggacggg
    11161 taaggagcag cggatcacca tcaccgcctc cagcggtcta tcggatacgg atatagagca
    11221 gatggtccgt gacgcggagt cgcacgctga cgaagaccgg cgccgccgcg aggaggctga
    11281 tgtccgcaac agcgccgaca acctgatcta caccgtcgag cgttcgctca aggacgtgga
    11341 cggtaaggtg gatggcgcta ccaagctgga gatagaaaac gccatcaaag aggctcgcga
    11401 agcgctcgcc ggcaacgacg tggaggagat caaggcgaag caggaagccc tgatgacggc
    11461 ttctcataag ctctccgaga cgctctacca gcaggcccag cagcagcaag aggctgctag
    11521 ttcctccggc accgagaacc cggatgagga tctggtcgag gatgctgagg tcatcgacga
    11581 ggacgacgaa gagcagcgca agtagcagca tctgtgtgga ggccgggggc tgtttcgccc
    11641 tcggcccccg attgcgacga gcacggagga gctttttgag caacgaagaa cgtaaacagg
    11701 gatcgccgga agaggagatc cagcccggag accagccggt cgaagagggc caggagttgc
    11761 cggaggtcgc ggcggaggag cctaaagcgg acaatagcgc cgcggccacg caggaggagc
    11821 ctgcggagac cggggaagac gctccgcagg atgagccgga ctctgatgca ggcacgctgg
    11881 aagaggtcac ggctctgtcg gagaagttgg agatcaccag ccgcgagcgc gatgattacc
    11941 tggacgccct gcgccgcctg aaggccgagt tcgagaactc gcgcaaacgc caggagcggg
    12001 agcggcagcg aatcctccag accgcttcgg agaggctgat cacggagctg ctgcccgtcc
    12061 tggataacct ggatcgcgct ctggaccacg agggtgacat ccgggagggg gtgcgcgcca
    12121 cccgcgatca gctcgccgat gtcctcggcc gcgagggtct gcttccggtc gcctccgacg
    12181 gccagagctt tgaccctaac gtccatgagg cggtaatgag ccagccctcc gaggaccacg
    12241 aggaagggac catcatccag accttcgagc ggggctatgt gctcaacggt aagcccatac
    12301 gtgccgccaa ggttgtagtt gcttctcaag gttagagatg cagactaagg atctctacaa
    12361 ggttctgggg gtagagaagg aggcttcgca ggatgacatc cggcgcgcct accgcaagct
    12421 ggcgcgcaag caccacccgg acgccaaccg cgacgacccc aaggccgagg accgcttcaa
    12481 ggagatccaa tcagcctacg aaatcctctc ggaccccaat aaacgccggg aatacgacga
    12541 gggtccccgc acgttcttcg gccagggagc cggacgtccc ggcgccagag gcgctggcac
    12601 aacgggcgcc agtcaggccg acttctcgga cctcttcggt accttcggtg acatcttcgg
    12661 ggccggagcg agcgcccgga gccagcccgc cgcaacccgc ggcaacaaca taacggtaaa
    12721 cgtaaacctg aaattcaaag atgccttgaa tggcgttacg accagggttg cggttccggt
    12781 ggaggaggtt tgtggtgatt gccgggggac gggggctgct ccggggacga tgccgaggac
    12841 ctgtccggac tgcggcggcc tggggacgca gagccgggac caggggttct tcgcgctctc
    12901 gacttcctgc tcgcggtgcg ggggcgaggg gacaatagtg gagaagccgt gtcccaggtg
    12961 cgcggggcag gggagagtca agaagacgcg gcaggtcacg gtcaaaatac cggctggcgc
    13021 gcgggatggg atgaagatcc gggtccctgg aaggggcagc gcggggcaga agggtggtcc
    13081 ggcgggcgac ctgtacgtgg tcaccagggt cgaggagcat ccggtattca agcgtagggg
    13141 ggatgatttc gtggtcgagg tgccggtcag cttcgcggag gcggcgctcg gggcggagat
    13201 ccgggtcccg aagcccgacg gtgggacggt caagctgcgg ctgccggcgg ggacgcagga
    13261 gggacggcag ttcaaggtgc gcggggccgg ggctccgagg ccaaagagtg ggggacaggg
    13321 cgacctcatc gtgcgggcga agatcgtggt ccccaagaag ctcaaccggc gcgagcggga
    13381 catcctggag gcgctcgccg aggaacggga tgaggacgtg cgggaagacc tgttcaggag
    13441 ggtagggtcg tgagcttcag gaagcggccg gtgttcatga tcggggtggc ggcggagctt
    13501 ataggggtac atccgcagac cctcaggatg tacgagcaga agggattgct gcgcccgcgc
    13561 aagagcataa agaacacccg gctctactcg caggaggatg tcgagcttgg acgttacatc
    13621 cagaagctca cccaggagat ggggatgaac ctcgccgggg tggggaaggt gctggatctg
    13681 gagcgcaagc tgg
//
LOCUS       DASSMB010000239         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_580796, whole genome shotgun sequence.
ACCESSION   DASSMB010000239 DASSMB010000000
VERSION     DASSMB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580796"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFJ72_13835"
     CDS             complement(<1..210)
                     /locus_tag="VFJ72_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480582.1"
                     /translation="MARNRLLGVLAVAAAACTWGTLGFFAKILYAEGVSFEALVAFRA
                     VVGWVAVVLFVLATGGARSLRVPGRD"
     gene            complement(213..1376)
                     /locus_tag="VFJ72_13840"
     CDS             complement(213..1376)
                     /locus_tag="VFJ72_13840"
                     /inference="COORDINATES: protein motif:HMM:NF018485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480583.1"
                     /translation="MSGARRLGLAGFFAIAVAFGPARNGYGLFLPDIRSEFGLSTELS
                     GIIASASQAGYLISLSLVGLLVGRLGPRIPVVIGGLSAGLGMGLVALAPNAAVLAIGF
                     VLAATYAGWSWAPYNDAADLMVAQRYRDRVLSVISTGTTFGILMAGLAALAASADWRT
                     AWLVFSGAALAATALNAWFLPGGSHEAPGQAPRPRPKLGWLVRSESAPLFVTAFSFGL
                     VSAFYWAFAVDLISRSVTFAGTGPIFYVVLGIVGFVGVFTGDAVSRFGLRRVLLAILL
                     ALAAAAFLLGVAPGWWPAVGASAILYGACVMFMSALLAVWSSAVFAEQPSTGFSATLV
                     LFGIGSTIGPATLGAFASGFGLEAAFLMSATLALLPMLVLPTKELHPQTEQSS"
     gene            complement(1408..>3141)
                     /gene="mutL"
                     /locus_tag="VFJ72_13845"
     CDS             complement(1408..>3141)
                     /gene="mutL"
                     /locus_tag="VFJ72_13845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET7480584.1"
                     /translation="PASVVKELVENALDAEASRIEVELGEGGTASILVRDDGSGMSAE
                     DARLSVLRHATSKIQTVEDLESVMTMGFRGEALPSIASVSDFTMTTSTGAGAGTRVAV
                     AGGAEAEVSPASHPRGTTVLVDRIFYNVPARRAFLKGSRAERAAILETLTHIAIAHPD
                     VAFRLTEKGRDYLSLPAANDLLERLAQILGVAKARAMRRVEYESGAFEVRGYAALPSI
                     TEGSRAHQTVSVNGRWVRAENLNRGLDDAYRATVPSGRYSPVALEMKVDPRRVDVNVH
                     PTKQIVRFSEERAARAAIASAVRSAIEWRPPAPTTAPRQSERTFTQVRFTVPSAAPQT
                     PPRESYPAFAAESRPVYPTPPSRSLQEARERISEASRPLDELAFPVEAPERGALPPLE
                     DLRVIGQLAAGYILVEEPESLWIVDQHVAHERAILDRLNDSESPATVQNLLIPEVVEL
                     SAGEAEMAADSLEELAVYGFEAEPFGPRSVRVTAVISTLADRDVAGAFKDALSAVSGT
                     GPGHAREDRILATIACHSAVKMGDRMSHPEMEALIKDWLTSRLPATCPHGRSICYRIG
                     LNEVARKLDRH"
BASE COUNT          554 a         1079 c         1034 g          474 t
ORIGIN      
        1 gtctctgccc ggcactcgca ggctccgcgc tccgccggtc gccaggacga agaggaccac
       61 ggccacccac ccgacgaccg cccggaacgc caccagcgcc tcgaacgaga cgccctcggc
      121 gtagagtatc ttcgcgaaga accccagcgt tccccacgta cacgctgcgg cggcgacggc
      181 caggacgccc agcagcctgt ttcgcgccaa gttcaagagc tttgttcggt ctgtgggtgt
      241 agctctttag tgggcagtac gagcatgggc agcagtgcga gtgtggccga catgagaaac
      301 gccgcctcaa gtccaaatcc gctggcgaat gcgcccagcg tcgcgggtcc tatcgtgctg
      361 ccgatcccga agagtaccag cgtcgcgcta aagccggtcg acggctgctc ggcgaatacg
      421 gccgaactcc acaccgccag cagagcgctc atgaacatga cgcacgcccc ataaaggatc
      481 gcggaggcgc cgaccgccgg ccaccacccc ggcgctacgc ccaacaaaaa ggccgcggcg
      541 gccagggcga ggagtatggc gagaagcacc cgacgcaacc caaagcggct caccgcgtcc
      601 ccggtgaaga ccccgacgaa gcccacgatc cccaagacga cgtagaagat cggtcccgtc
      661 cccgcaaacg ttacgctgcg ggagatcagg tccaccgcga aagcccagta gaaggcgctg
      721 accagcccga aggagaacgc cgtgacgaac agcggcgccg actcgcttcg caccaaccat
      781 ccgagcttcg gtcgaggtct cggagcctgt ccgggtgcct cgtgggatcc gccgggcaga
      841 aaccaggcgt tcagagccgt tgcggcgagc gcggcccccg agaagactaa ccaggcggtg
      901 cgccaatcgg cgctcgccgc gagcgccgcg aggccagcca tcaggattcc gaatgtcgtc
      961 cccgtgctta tgaccgacaa gacgcggtcc ctgtaccgct gcgccaccat gagatcggcg
     1021 gcgtcgttgt agggcgccca ggaccagccc gcgtaggtgg ccgccaagac gaaccctatc
     1081 gcgagcacgg cggcgttcgg agccagggcg acgagcccca tccccagacc cgccgatagt
     1141 cctccgatta ctaccgggat gcgaggtccc aggcgaccga cgagcagccc aacgagggac
     1201 agcgagatca ggtagccggc ctgagaagca ctggcgatga tccccgaaag ctccgtggat
     1261 aatccgaatt cactacgaat atcgggcaag aacagcccat agccgttgcg cgccggtccg
     1321 aaggccaccg cgatagcgaa gaaccccgcc agacccaggc gcctggctcc actcaccgct
     1381 caacctcgat cgctacggca tcgtacatca atgccggtcg agcttgcggg cgacctcgtt
     1441 caacccgatc ctgtagcaga tcgaacgccc gtgcggacag gttgctggca gccggctcgt
     1501 cagccagtcc tttatgagcg cctccatctc gggatgcgac atcctgtcgc ccatcttgac
     1561 ggcggagtgg caggcaatgg tagcgaggat tcggtcttcg cgcgcgtgtc cgggtccggt
     1621 acccgagacg gcggagagag catccttgaa ggcgcctgcc acgtcccggt cggcgagcgt
     1681 cgagatcacg gccgtcacac gcacggaacg cgggccgaag ggctcggcct cgaagccgta
     1741 gacggcgagt tcttccaggc tatcggccgc catctccgct tcgccggcgg agagttctac
     1801 gacctcgggg atgaggaggt tctgcacggt ggccgggctc tcggagtcgt tgagcctatc
     1861 caggatcgcc cgctcgtgag ccacgtgctg gtccacgatc cacagcgact ccggctcctc
     1921 cacgagtatg taaccggccg cgagctggcc gatcacgcgc agatcctcca gcggcggcag
     1981 cgcgccccgc tccggcgcct ccaccgggaa cgccagctcg tcgagcggtc tcgaagcctc
     2041 ggagatgcgc tcgcgcgcct cctgcaagct tctcgaaggc ggcgtgggat acacgggtcg
     2101 gctctcggcg gcaaacgcgg gatagctctc tcgcggcggg gtttgtggcg cggcgctcgg
     2161 gacggtgaag cggacctgag tgaatgtcct ctccgactgc ctcggcgcgg ttgtcggggc
     2221 gggaggacgc cactctatcg cgcttcggac tgcggaggcg attgcggcgc gggctgcgcg
     2281 ctcctcggag aaccgcacga tctgctttgt cggatgcacg ttcacgtcca cgcggcgcgg
     2341 gtccaccttc atctccagag ccacgggcga atagcggccg gagggcacgg tggcgcggta
     2401 ggcgtcgtcc agaccgcggt tcaggttctc cgcccggacc cagcggccgt tcacggagac
     2461 tgtctggtgc gcgcggctgc cctcggtaat actcggcagg gccgcgtacc cccgtacctc
     2521 gaaggcgcct gactcgtact cgacgcggcg catggcgcgg gccttcgcca cgcccagtat
     2581 ctgggcgaga cgctccagca ggtcgttcgc ggctggtagc gagaggtagt cgcggccttt
     2641 ctccgtgagg cgaaaggcaa cgtccggatg ggcgatggct atgtgcgtga gggtctccag
     2701 tatggcggcc cgctcggcgc gcgatccctt gaggaacgcc cgccgcgccg ggacgttgta
     2761 gaagatgcgg tccaccagga ccgtcgtgcc ccttgggtgg gaggcggggg agacctcggc
     2821 ctcagcgcct cccgcgacgg caactctcgt gcccgcgccc gcgccggtcg aggtggtcat
     2881 cgtgaagtcc gagacgctcg ctatagaggg gagcgcctcg ccccggaagc ccatcgtcat
     2941 cacggactcc aggtcttcga cggtctggat cttgctggtc gcgtggcgaa ggacggagag
     3001 gcgggcatcc tccgcgctca ttccggagcc gtcgtcgcgc accaggatac tggcggtacc
     3061 gccttctcct agctctacct cgatgcgtga ggcttcggcg tccagagcat tctcgaccag
     3121 ttccttcacc acggacgccg g
//
LOCUS       DASSMB010000240        10292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_191949, whole genome shotgun sequence.
ACCESSION   DASSMB010000240 DASSMB010000000
VERSION     DASSMB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10292
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191949"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VFJ72_13850"
     CDS             <1..540
                     /locus_tag="VFJ72_13850"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7480585.1"
                     /translation="RSLRGYESFTSVAATAEATTMEDHSVDFVAAGQAFHWFEPGRAR
                     TEFIRILRPGGWTVLVWNTRRGQGKRALFLQAYERLLDTYGTDYGEVGHGRHGSQEEV
                     RAFFAPSPFEAATFDNRQVLDLCGLKGRLLSSSYVPAEGEPNSAEMLRDLERTFHEHQ
                     ENGAVTIEYDTRVYYGRLW"
     gene            complement(595..1065)
                     /locus_tag="VFJ72_13855"
     CDS             complement(595..1065)
                     /locus_tag="VFJ72_13855"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7480586.1"
                     /translation="MVKHIVLLAGTVVLTLTLTGCGGAAGSSSGGKSSGTSGTGGGST
                     TKSAIVKTVVVKESEFKLVPSSITLKKPGTYVFKAENVGSASHALEIEGNGVETETKV
                     LDAGKSGGIQVKLKPGNYEMYCPVDNHKDNGMKGKITVAGDGDSGGGGGPGGGY"
     gene            1201..1725
                     /gene="ruvC"
                     /locus_tag="VFJ72_13860"
     CDS             1201..1725
                     /gene="ruvC"
                     /locus_tag="VFJ72_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET7480587.1"
                     /translation="MITATGGKGQTILGIDPGTAIMGWGVVRQEGNRLRYVQHGAITT
                     PSDWEMPRRLSRLFDGVTELVRGYRPETVAVEELFFNTNVTTGITVGQARGVALLAAY
                     RAGIEVAEYTPLQVKQAITSYGRADKRQVQEMVKTLLNLPAIPRPDDAADGLAIAITH
                     AFSSRMGGKVGVGK"
     gene            1750..2373
                     /gene="ruvA"
                     /locus_tag="VFJ72_13865"
     CDS             1750..2373
                     /gene="ruvA"
                     /locus_tag="VFJ72_13865"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7480588.1"
                     /translation="MIHRLRGQLVEKDAEGVVVDVVGVGYRTATSQGTLRALPAIGEE
                     CVLHTRMVVREDAMLLFGFATRDERAAFDALVTVSKVGPKLALSVLSSMSPPEIAECV
                     ARGDIVKLASVPGLGRKTAERLVLELRGKDLVAAFAPEPSSANAGSSSGGGHGGGGPF
                     MEAREALTALGYSLDEAEGALKEVPPQDTVEKYIKEALRRIGSRRRA"
     gene            2370..3494
                     /gene="ruvB"
                     /locus_tag="VFJ72_13870"
     CDS             2370..3494
                     /gene="ruvB"
                     /locus_tag="VFJ72_13870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7480589.1"
                     /translation="MTGMEEMDDFTVRGDGRSGEPPEAPEDITGAASGFGAVDDALDP
                     ERFEGDDEPKLRPATLDEFIGQERLKDNLRIFVEAAKRRGEALDHVLLAGPPGLGKTS
                     LCRILAAEMEVNLSTTSGPSLERAGDMAAILTSLEEGDFLFIDEIHRLNRQIEEVLYP
                     AMEDFAMDIVLGQGPSARTIRMDVPKFTLVGATTRTGLMTKPLLDRFGFSARLEYYEP
                     AELEKIVVRNSKILNIPVTQDGARQLATRSRGTPRIANRLLRRVRDYAEVVGDGQIDE
                     ETAKAALAMQGVDDLGLDRADRDYLGLIIDKFDGGPVGVGTISVALGEARDTVEDVYE
                     PYLLQSGLIQRTARGRIATHRAYNHLGVARPDAENNLWSD"
     gene            3716..4009
                     /locus_tag="VFJ72_13875"
     CDS             3716..4009
                     /locus_tag="VFJ72_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480590.1"
                     /translation="MSRKKYAGFEETTALLEEHLPERPYVRFTDLRRNGRLVFSQLWA
                     GFEGTEECATAGWRVRIPINPARVRRDPQGSAEFIIRKVDDAARNAGVFYWEI"
     gene            complement(4062..5003)
                     /locus_tag="VFJ72_13880"
     CDS             complement(4062..5003)
                     /locus_tag="VFJ72_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7480591.1"
                     /translation="MLKNPLQSHQTLEKAYAALGRVPLFEGVRRDNVEIEPLGSLTNS
                     SYKITTSGGAYVLRLPGDSTSEYIDRVAEEHNSRIAAAAGVGAEVVYFDTMTGAMVSR
                     FIQGVNMDNDHFHQDPEAPARAALVLRRVHGIGPVFKYRFNAFGMIDHYLDLLRGMRM
                     PPPEGYYGVEKRMEAVRQALEVLPASLASCHNDPWPPNFIDTGSRMYLIDWEFSGMND
                     PYWDLAHLSVEAGFGAGQDHAMMQAYTDGAATLESYSRLELYKATSDLHWSLWALVEH
                     ASGNTTFDFRAYSQRRFDACRARIDTTDFGRSLAAVR"
     gene            complement(5087..5920)
                     /locus_tag="VFJ72_13885"
     CDS             complement(5087..5920)
                     /locus_tag="VFJ72_13885"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7480592.1"
                     /translation="MSSYENYTEASRHYDRTREPVGTEIIVGCMAHAPVPLSSTTILD
                     AGCGTGSYSRALLDYVGRIEAVDLNDGMLEVAGNKLARARQEDRIAFTSSRIDELPFG
                     DGSLDGVMINQVLHHLPDDASDGFPEHGKVFREFARVLRPGGVLTVNTCSQQQLRHGY
                     WYYDLIPRGAQTLRERFAPITELKRLLQDSGFDYRGRFTPTDATIQGDSYFDPRGPLS
                     QEWRNGDSTWSLVREDELRQALSKVRELEERGEIEDYLSRNDARRKDIGQITILFAIR
                     Q"
     gene            6064..6972
                     /locus_tag="VFJ72_13890"
     CDS             6064..6972
                     /locus_tag="VFJ72_13890"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7480593.1"
                     /translation="MAPGGVGINRVGGIAGLPSELEALKSGGADFVELWVGELGVIRG
                     GNLDEARLRPVRESLLDADLAYTVHSPIELNLMQLAGADLHRDVLATSIRFAGEIGAK
                     VVVCHAGQRSWSRDARVSFKDQLAAERYALREAGDLAADLGVSIAVENYYPDMSVLSG
                     AVYDYSVRPSELADQVSGVDHPSIGVCLDVGHAALAANAFGFDLIEECAAVSPLVRHL
                     HLHDNLGRTDLDVDPLSYGNPVYGIGDLHLPPGSGSIPLEDLFRRVDFPRDPARCVEL
                     APELVSETREALNAARRLTTRERVIS"
     gene            complement(7137..7625)
                     /locus_tag="VFJ72_13895"
     CDS             complement(7137..7625)
                     /locus_tag="VFJ72_13895"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HET7480594.1"
                     /translation="MNYAPTPYALGSEDGEALWFFGTLLTVKAGAEQTGGRFALVEQY
                     APRGMATPLHIQPEDDETFYVLEGDLTFYIEDGQPIPAPAGSFVHIPAGTPHAFQVDS
                     KTARFLDLTTPQHESFMRAAGEPAQERVLPPPGPPDMERVGAAAQKYGVDIVGPPPGA
                     PA"
     gene            7893..8312
                     /locus_tag="VFJ72_13900"
     CDS             7893..8312
                     /locus_tag="VFJ72_13900"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7480595.1"
                     /translation="MQNVAVVGQIYEAFGRGDVPAILDHLAEDVEWEYGVDSTDVPWL
                     QPRRGRQEVPGFFEALGALEIHSFAPKTMLESDDVVVAIVDFEATVKETGQRISEEDE
                     AHIWHFAEGKVVRFRHRSDTHQHQLAYQGQGVSGAIR"
     gene            8411..>10292
                     /locus_tag="VFJ72_13905"
     CDS             8411..>10292
                     /locus_tag="VFJ72_13905"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HET7480596.1"
                     /translation="MTKMTPQTFASKWSEAALSERSSYQQHFLDLCAMLGQPTPAEVD
                     PTGEFFTFERGVEKMGGGRGFADVWYRGHFAIEYKGKHKDLGAAYVQLQNYREALENP
                     PLLVVTDMERFEVHTNFESTVSRVYSFTNAELPESENLRVLRAMFEDPASLRPARTVE
                     SVTEEAAGKFARIADGLRGRGVEPHAAAHFLNKLLFCLFAEDIGLLPKGLFTQVVERG
                     VRRPETFNANVGGLFAAMRDGGEFALQDIPRFNGGLYSEEDEESAVVPLAAEELRLLA
                     EAARLDWESVEPAIFGTLFERSLDPSQRARLGAHYTSREDILAVVEPVLMAPLRREWD
                     AVRERAEADAEAARKASGQKAANAQRSAERALVEFAGRLRRVRVLDPACGSGNFLYVS
                     LKELLDLEKEVYTFAGSIGLTQFFPEISPEQLYGIETSPYAHELAQVAVWIGYLQWMH
                     DNGFGTRQEPILGNMTNIVEMDAILARDEDGNPTEPDWPEADVIVGNPPFLGGNKIRA
                     ELGGEYVESLFELYKGRVPAFADLVCYWFERAREQIETGKVRRAGLLATNSIRGGVNR
                     RVLERIKESGDIFFAESDRPWILNGAAVRVSMVGFDSGTETAKILDGVPADTIHANLT
                     ATA"
BASE COUNT         1792 a         3012 c         3625 g         1863 t
ORIGIN      
        1 cggtccttgc gggggtacga atcctttacg agcgtcgcgg ccaccgcgga ggctacgacg
       61 atggaggacc acagcgtcga cttcgtggca gcgggccagg cgttccactg gttcgagcct
      121 ggccgggcgc ggacagagtt cattcgtata ctcaggcccg gtggctggac cgttctcgta
      181 tggaatactc gccgcgggca ggggaagcga gccctgttcc tgcaggcgta cgagcggttg
      241 ctggacactt acggcacgga ctacggggag gtcggacacg gccgtcatgg gagtcaggag
      301 gaggtccggg ctttcttcgc tccctcgccg tttgaggcgg cgacgttcga taaccggcag
      361 gtcctggacc tctgcggcct gaagggacgg ctgctgtcgt cgtcctacgt gcccgccgag
      421 ggtgagccga acagcgcgga gatgctacgg gatctggaga ggaccttcca cgaacaccaa
      481 gagaacggcg cggtgactat cgagtacgac acgcgagtct actatgggcg tctttggtga
      541 ttcgatctag aaaccctgct gcgtcccgcg tagaaacgga tgatatgcgc cgggctagta
      601 gccacctccc ggccctccac cgcctcccga gtctccgtcg ccggcgaccg tgatctttcc
      661 tttcatccca ttgtctttgt ggttgtcgac tggacagtac atctcgtagt tgccaggctt
      721 gagctttacc tggatcccac cgctcttgcc cgcgtccagc accttcgtct cggtctccac
      781 gccgttacct tcgatctcca gggcgtgact ggcgctccca acgttctcgg ccttgaagac
      841 gtaggtgccg ggtttcttca aggtaatgct tgacgggacc agcttgaact ccgactcctt
      901 gaccacgacc gtcttgacga ttgcgctttt ggtcgtggag ccgccgcctg tgccggacgt
      961 tccgctactc ttcccgccgg aactgctgcc tgcggccccg ccacatccgg tcagcgtcaa
     1021 tgtcagaact accgtgcccg ccagcaggac gatgtgcttc accattgctc ctctctttcc
     1081 cctgcgggtt tatacgcgcg gggcggcggt ctggatctgg ctgtgttgtg gatcgcggat
     1141 ggcggagccc catatgtgga acacggtgca aggcgggtgg gtagcgagta cccttagcgc
     1201 atgatcacgg ctaccggcgg caaggggcaa actattctgg ggatagatcc cggcacggcc
     1261 attatggggt ggggcgtggt acggcaagag gggaaccggt tgcgctacgt tcagcacggc
     1321 gcgattacga ctccttcgga ctgggagatg ccgcgccggc tctcgcggtt gttcgacggc
     1381 gtgactgagc tggtccgggg ttaccggccg gagacggtgg cggtggagga gcttttcttc
     1441 aacacgaacg tcacgacggg gatcacggtc ggccaggcgc ggggcgtcgc gctgctggcg
     1501 gcctaccggg ccggtatcga ggtggcggag tacacgccgc tacaggtgaa gcaggcgatc
     1561 acctcctacg gacgggcgga taagcggcag gtgcaggaga tggtgaagac gttgttgaat
     1621 ctgccggcga tcccgcgtcc cgacgacgcg gcggacgggc tcgccatcgc catcacgcac
     1681 gccttctcgt cccggatggg cggcaaggtc ggggtgggga aataacggtt tgtgcgggat
     1741 aattagcgca tgatacatag actgcgcgga caactggtcg agaaggacgc cgagggcgtc
     1801 gtggtggacg tggtcggcgt cgggtaccgg accgcgactt ctcaggggac gctgcgggcg
     1861 ctaccggcta tcggggagga gtgcgtgttg cacacccgga tggttgtgcg ggaggacgcg
     1921 atgctcctgt tcgggttcgc cacccgcgac gagcgggcgg cgttcgacgc gctggtgacc
     1981 gtgagtaagg tcgggcccaa gctggcgctc tcggtgctct cctcgatgtc gccgccggag
     2041 atcgccgagt gcgtggcgcg gggggatata gtgaagctgg cctcggtgcc ggggctgggg
     2101 cgcaagacgg cggagcggct ggtgctggag cttcggggca aggacctggt cgcggccttc
     2161 gcgccggagc cgtcgagcgc gaacgccggg agcagcagtg gtggcggcca cggtggcggc
     2221 gggccgttca tggaggcgcg ggaggcgttg acggcgctcg ggtattcgct ggacgaggcg
     2281 gagggcgcgc tgaaagaggt gccgccgcag gatacggtcg agaagtacat caaggaggcg
     2341 ttacggcgga tcggaagcag gcgccgggca tgacgggcat ggaggagatg gacgacttca
     2401 cggtacgcgg cgatggacga agcggagagc cgccggaggc gccagaggac ataaccggag
     2461 cggcttcggg tttcggagcc gtggatgacg cgctggaccc ggagaggttc gagggggacg
     2521 atgagccgaa gctgcgtccg gcgacgctgg acgagttcat cggacaggag cggctgaagg
     2581 acaacctccg gatcttcgtg gaggcggcga agcggcgcgg ggaggcgctg gaccacgtgc
     2641 tgctggcggg accgccggga ctggggaaga cgagcctgtg ccggatactg gcggccgaga
     2701 tggaggtcaa cctctcgacg acgagcggac cgagcctgga gcgggcgggg gacatggcgg
     2761 cgatcctcac gtcgctggag gaaggcgact tcctgtttat agatgagatc caccgcctca
     2821 accgccagat agaagaggta ctctacccgg cgatggagga cttcgcgatg gatatagtgc
     2881 tcggccaggg gccgtcggcg cgcacgatac gcatggacgt cccgaagttt acgctggtgg
     2941 gggcgacgac gcgcacgggg ctgatgacga agccccttct tgaccgattc gggttctcgg
     3001 cgcggctgga atactacgag ccggcggagc tggagaagat cgtggtccgc aactcgaaga
     3061 tcctgaacat acccgttact caggacggcg cgaggcaact ggcgacccgc agccggggca
     3121 cgccgcgcat cgccaaccgg ctcctgcggc gcgtccggga ctacgcggag gtcgtgggtg
     3181 acggtcagat cgacgaggag accgcgaagg cggcgctcgc gatgcagggc gtggacgacc
     3241 tgggtctgga ccgcgcggac agggactacc tgggactgat catagacaag ttcgacggcg
     3301 ggccggtggg ggtcgggacg atctcggtgg cgctcggcga ggcgcgcgat acggtcgagg
     3361 atgtctacga gccatacctg ctccagagcg gcctgatcca acggaccgcc cgcggacgca
     3421 tcgccaccca ccgcgcatac aaccacctcg gcgtcgccag gcccgatgcg gaaaacaacc
     3481 tctggagcga ctgaaccgca ctgcctgtga gctggttcac cgcaagggtc ctgtttccgt
     3541 tgcggcgctg tggtgtacgc gtaacggcgg tgatatgctg gctggagagt ttagtattgc
     3601 ggtcctccga ggaggcgggc cgagagagcg aggttccggg gatgcagagt ttgggaagcc
     3661 gtttcgggaa gcggcgggtt gcgcgtttga agccgcggag cgggaggagt tgagggtgag
     3721 ccgtaagaag tacgcgggtt tcgaagagac gacggcgctg ctggaagagc accttccgga
     3781 gcggccctac gtccggttca cggacctccg ccgcaacggg aggcttgtct tctcgcaact
     3841 gtgggccggt ttcgagggga ctgaggagtg cgccacggcg ggctggcggg tgaggatacc
     3901 gatcaatccg gccagggtgc gccgggaccc gcaggggtcg gccgagttca tcatccgtaa
     3961 ggtggacgat gccgcccgga acgccggcgt gttctactgg gagatctaaa gcgagagctt
     4021 caggctgtgt gatgcgcctg aagaggcagg agcggtagtt tctaacgcac ggcggccaga
     4081 ctccggccaa agtccgtggt gtcgatccgc gcgcggcagg cgtcgaacct cctctgcgag
     4141 taggcccgga aatcgaaggt ggtgttcccg ctggcgtgct cgacgagcgc ccacagagac
     4201 cagtgcagat cgctcgttgc cttgtagagc tccaggcggg agtaggattc cagggtagcg
     4261 gcgccgtccg tgtaagcctg catcatcgcg tggtcctgac cggctccgaa gccggcctcc
     4321 acggagaggt gcgcgagatc ccagtaaggg tcgttcatgc cggagaactc ccagtcgatg
     4381 aggtacatgc ggcttccagt gtcgatgaag ttcggcggcc acgggtcgtt gtggcaggac
     4441 gccagggacg cgggaagcac ctccagcgcc tggcgcaccg cctccattct cttctcgact
     4501 ccgtagtatc cctcgggtgg cggcatccgc atgccgcgca ggagatccag gtaatgatct
     4561 atcatcccga aggcgttgaa gcgatacttg aagaccggcc cgatgccgtg aacccgcctt
     4621 agcaccaacg cggcccgggc cggcgcctcc gggtcctgat ggaagtggtc gttgtccatg
     4681 ttcacaccct gtataaaccg ggagaccatg gcgccggtca tggtgtcgaa gtagacgacc
     4741 tccgcgccca cgccggcagc cgccgctatc cgcgagttat gctcctcggc gacccggtct
     4801 atgtactcgg aggtgctgtc tcccggtagc cggagcacgt aggcgccgcc gctggtggtt
     4861 atcttgtagc tcgaatttgt cagagagccg agcggctcga tctccacgtt gtcccgccgc
     4921 acaccctcaa agagcggcac ccgacccagc gcggcataag ccttctccag ggtttgatga
     4981 ctctgtaacg ggtttttgag caagcgtctc caaagcccgt ctccgggtct tgttcgatat
     5041 tagtcttatg ttaacttatt gtgaaatatg cgtcaatgag gcgggatcat tggcggatgg
     5101 cgaacaggat ggttatctgg cctatgtcct tgcggcgggc atcgttgcgg gagaggtagt
     5161 cctcgatctc gccccgctcc tccagttccc ggactttcga gagggcttgc cgaagctcgt
     5221 cctcgcggac gagtgaccag gtcgagtctc cgttgcgcca ctcctggctc aaggggccac
     5281 gggggtcgaa gtaggagtcg ccctggatcg tggcatcggt cggtgtaaac cggccccggt
     5341 aatcgaagcc gctatcctgg aggagcctct tcagctcggt tatgggagcg aaccgctcgc
     5401 gcagggtctg agcgccgcgc gggatgaggt cgtagtacca gtaaccgtgg cgcaactgct
     5461 gctgggagca ggtattgacg gtgagtacgc cgccgggtct gaggacgcgg gcgaactccc
     5521 ggaacacctt gccgtgttcg gggaaaccgt ccgaggcgtc gtccggcagg tggtggagga
     5581 cctggttgat catgacgccg tcgagcgagc cgtcgccgaa gggcaactcg tctatccggg
     5641 aggaggtaaa ggcgattcgg tcctcttgcc tcgcgcgggc cagcttgttg cccgcaacct
     5701 ccagcatgcc gtcgttcagg tctaccgcct cgatgcggcc gacgtagtcg aggagggcgc
     5761 gggagtaact gccggtgccg cacccggcgt cgagtatcgt cgtgctactg agcggcacgg
     5821 gcgcgtgagc catgcacccg acgatgatct cggtcccgac cggctctcgt gtccggtcgt
     5881 agtgcctgga ggcttccgtg taattctcgt agctgctcat ccgatccctt ccctcttttc
     5941 tcccgcgcgg gcgatacaaa gtgccgttaa accgaggttt tacacttgtg taacgctctt
     6001 gttacccgag cctcgaaccc tctcaatata ctagccgcgg ctctcacagg gaggacagac
     6061 cgtatggctc ctggcggcgt ggggatcaac cgtgtgggcg gcatcgccgg cctccccagt
     6121 gaactcgaag ctctgaagtc cggtggagcg gacttcgtgg agttgtgggt gggtgaactg
     6181 ggtgtcattc gcggaggaaa tctggatgag gctcgtctgc ggcctgtccg ggagtccttg
     6241 ctggatgcgg acctcgcgta tacggtgcat tctccgatag agttgaacct aatgcagctg
     6301 gccggggcgg acctgcaccg ggacgtcctc gcgacgagca tccggttcgc gggggagatc
     6361 ggggcgaagg tcgtagtctg ccacgccgga cagcggtcat ggtcgcggga cgcgcgcgtc
     6421 agcttcaagg accagctcgc ggcggagcga tacgccttgc gggaggcggg ggaccttgcc
     6481 gcggacctgg gcgtgagcat cgccgtcgag aactactatc ccgatatgag tgttctaagt
     6541 ggcgcggtgt acgactactc cgtgcggcct tcggagctgg cggatcaggt ctccggggta
     6601 gaccatccat ccatcggcgt ctgcctggac gttggacacg cggccctcgc ggctaacgcc
     6661 ttcgggttcg acctcattga agagtgcgcc gccgtgtcgc cgctggttcg tcaccttcac
     6721 ctgcacgaca acctgggaag gacggacctc gacgtggacc ccctctccta tgggaatccg
     6781 gtctacggga tcggagacct ccacctgcca ccgggcagcg gctccatccc gctcgaagac
     6841 ctgttccggc gggtagactt cccgcgcgac cccgcccgct gcgtggagct cgcccccgaa
     6901 ctcgtgtccg agacccgcga agctctaaac gccgcccgcc ggctgacgac ccgcgagcgc
     6961 gtcatttcct gactagctga ttgatccagc gccgatgctc caagcagttg caaagccccg
     7021 gcgcctggcc gcaatcgcgc cgccgaaaca atctcttccg cggaaataga ggctgtccgt
     7081 tgtccccggc tctaaagatt tggtccgagt cgcctgatga acaccttaca gttttgctag
     7141 gcgggggcgc cgggcggcgg accgactatg tctaccccgt acttctgggc ggcggccccg
     7201 actctctcca tgtccggcgg tcccggaggt gggaggaccc tctcttgcgc cggctctcca
     7261 gcggcgcgca tgaagctctc gtgttggggc gtggtgagat ccaggaaccg cgcggtcttc
     7321 gagtctacct ggaaggcgtg cggagtcccc gccgggatgt ggacgaaaga ccccgcgggg
     7381 gccggtatgg gctggccgtc ctcgatatag aaggtgaggt ccccttctag cacgtagaac
     7441 gtctcgtcat cctccggctg gatgtggagc ggcgtcgcca tcccccgggg cgcgtactgc
     7501 tctacgagag cgaaccgccc gccggtctgc tcggcgccgg ccttgacggt cagcagggtg
     7561 ccgaagaacc acagcgcctc tccatcctct gaaccgagcg catagggtgt tggtgcatag
     7621 ttcacgggat cctcctccac tcgcctgctg aaaacctgct gactggcaca ccgtaattct
     7681 gccggcgttt gcagtttggc gtgtcctccg aatgaatgac ttttcgtaac cgggacaaaa
     7741 tgtctacagc agacctgctc ctaacgcatt gacataataa gtgcaatatt caagaatggg
     7801 cactggatgg aaaggacagt ccgcgcgttc gggcgccagc ggcgatcggt ccgttgcacg
     7861 aacgtcgggc gtaatcaggc aaggagctgg atatgcaaaa tgtagccgtg gtcgggcaga
     7921 tctacgaggc attcggaagg ggcgacgtcc ccgcgatcct ggaccatctc gcggaggatg
     7981 tggagtggga gtatggcgtg gactcaacgg acgttccctg gctgcaaccg cgccgcggac
     8041 gccaggaggt gccgggtttc ttcgaggctc taggcgcgct ggaaatacat agcttcgcgc
     8101 cgaagacgat gcttgagtcg gatgatgtcg tggtcgctat tgtagatttc gaggccaccg
     8161 tcaaggagac cggacagagg atcagcgaag aggacgaggc ccatatctgg cacttcgcgg
     8221 aggggaaggt cgtccggttc cggcaccggt cggacaccca tcaacaccag ctcgcctacc
     8281 agggccaggg cgtatccggc gcgattcgct gactgagccc gatcatccgg gcggcgttgg
     8341 ttacgctcat ctggtagccg acgaccggga cgctggccga ataggggaga atgctggtag
     8401 catattaggc atgacgaaga tgacgccgca gacgttcgcg agcaagtggt cggaggcggc
     8461 gctctcggag cggtcgtcgt atcagcagca cttcctggac ctgtgcgcga tgctggggca
     8521 gcccacgcca gccgaagtgg acccgacggg cgagttcttc acgttcgagc ggggcgtgga
     8581 gaagatgggc gggggccggg gcttcgcgga cgtgtggtac cggggacatt tcgccatcga
     8641 gtacaagggg aagcacaagg acctgggcgc ggcctacgtg cagctccaga actaccggga
     8701 ggcgctggag aacccgccgc ttttggtggt gacggacatg gagcggttcg aggtccacac
     8761 gaacttcgag agcaccgtct cccgcgtcta cagcttcacg aacgcggagt tgccggaatc
     8821 ggagaacctg cgcgtgctgc gggcgatgtt cgaggacccg gcctcgctgc gcccggcgcg
     8881 gacggtcgag agcgtaaccg aagaggcggc ggggaagttc gccaggatcg ccgacggcct
     8941 tcgcggccgg ggcgtggagc cgcacgcggc ggcacacttc ctgaacaagc tgctgttctg
     9001 cctcttcgcg gaggatatcg ggctgctgcc gaagggactt ttcacgcagg tcgtggagcg
     9061 cggggtgcgc cgcccggaga cgttcaacgc caacgtcggc ggcctgttcg cggcgatgcg
     9121 cgacggggga gagttcgcgc ttcaggatat cccgcgcttc aatggcgggc tgtattcgga
     9181 ggaagacgag gagagcgcgg tggtgccgct ggcggctgag gagttgaggc tgctggcgga
     9241 ggcggcgcgg cttgactggg agtcggtgga accggcgatc ttcggtacgc tcttcgagcg
     9301 gtcgctggac ccgtcgcagc gcgcccggct cggggcgcac tacacgagcc gcgaggacat
     9361 cctggctgtt gtggagccag tgctgatggc tccgctgcgg cgagagtggg acgctgtgcg
     9421 cgagcgggcc gaggccgacg cggaggcggc ccgcaaggcg agcgggcaga aggcggccaa
     9481 cgcgcagcgg agcgcggagc gggcgctggt cgagttcgcc gggcggctgc ggcgcgtgcg
     9541 ggtgctcgac ccggcgtgcg gctcggggaa cttcctgtac gtgagcctca aggagctgct
     9601 ggacctggag aaagaggtgt acaccttcgc gggctccatc gggctcacgc agttcttccc
     9661 ggagatcagc ccggagcagc tatatgggat agagaccagc ccctacgcgc acgagctggc
     9721 gcaggtggcc gtctggatcg gctacctcca gtggatgcac gacaacggat tcggcacccg
     9781 acaggaaccc atcctcggca acatgaccaa catcgtcgag atggacgcga tcctggcccg
     9841 cgacgaagac ggcaacccga ccgaacccga ctggcccgaa gcggacgtga tcgtcggcaa
     9901 cccgccgttt ttgggtggta ataagattcg cgcagaactc ggcggtgagt acgtcgaaag
     9961 tctgttcgag ctttacaaag gtcgcgtgcc agcgttcgcg gatctcgtct gttactggtt
    10021 cgagagagca cgggagcaga tagagacagg caaggttcgg cgagccggac tcctagcgac
    10081 caattctatt cgaggtggag tgaaccggcg tgtgttggag cgcatcaagg agtctggaga
    10141 tattttcttt gccgaatcgg atcggccctg gattttgaac ggtgcggctg tgcgggtctc
    10201 gatggttggt tttgatagcg gcacagagac agccaagatt ttagatggtg tcccggctga
    10261 tacgattcac gccaatctga ccgctaccgc ag
//
LOCUS       DASSMB010000241         2492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_459256, whole genome shotgun sequence.
ACCESSION   DASSMB010000241 DASSMB010000000
VERSION     DASSMB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459256"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..1066)
                     /gene="secF"
                     /locus_tag="VFJ72_13910"
     CDS             complement(161..1066)
                     /gene="secF"
                     /locus_tag="VFJ72_13910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HET7480597.1"
                     /translation="MAFAKALSRVDFVGRWKTWFVLSGIFLLIGIGFIAVRGLNLGID
                     FEGGAKFNAASPKNDLTVGEVRAAMPPEHRQDAVIQRAGNNGWEVRTPVLSQPETSKV
                     RDALAKALGAEISVQSISPTFGGQVRDQALQAVGAALLVIVVFISFRFEFSFAMAAMV
                     ALLHDILMTIGFYAIVGREVNLVTVVAVLTILGYSLYDTIIIFDRIRENTPAVGYNKR
                     RFDDMINTSIRQVVTRSIFTSVSTLIPITALLIFGESTLSDFAFALLVGIIAGTYSSI
                     FIASPILSLYKAWRAGGSRRPARAT"
     gene            complement(1066..>2492)
                     /gene="secD"
                     /locus_tag="VFJ72_13915"
     CDS             complement(1066..>2492)
                     /gene="secD"
                     /locus_tag="VFJ72_13915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET7480598.1"
                     /translation="LLGVAAYLIFIRQPVGQSTKLGLDLRGGVSAQLQGSQTGGGKVT
                     RDEIEQAANLIRERIDRLGVSEPQVQVQGDDQVIVQIPGITNTERTLKIIGDTAQLGF
                     YQVLATDGRQSVPDDQIDKTRTEIRKSLEKDSAYKKDKTKILFEESPALQGKGTDVIG
                     YVVDKNPQLTGDALKQNGANVDTDPSTGKRIVTLELTREGGNKMSELTQKIINAKLAG
                     KPGNLAIALDEDVQSAPVVQDRLSQQISISNNGLPQGLPEQEAKQLVTVLNTGALPIN
                     MKVLSVTTIGPTLGTDSLKSGLLAALVGFGLVLIFLLVIYRALGLVADLALLIYALLL
                     WGLIVAIPVTMTLPGIAGIVLSIGVAADANIVIFERIKEEVRRGKTPRSAIALGYEKG
                     FRAILDGNLTTIIVAFILFALSSGSVRGFAVLLVIGVVLSMFTAIVVTRALLGLLSSR
                     GIHLSPGLMGVSKASISTRNERVS"
BASE COUNT          427 a          803 c          812 g          450 t
ORIGIN      
        1 tcttcgggca tatcggcctc ttctcttttc tcaggcgctc cggcacgacg acgagaaacg
       61 ccaccttttg cacttgagcg catcgtatcc acctatcggg cgaggcgtat cacccgttta
      121 gcgtattttc gaggaagaac cggccggctg aagtcggaga tcaggtagcg cgggcggggc
      181 ggcggctccc tccggcccgc cacgccttgt agagcgagag gatcggagag gcgatgaaga
      241 tggagctgta ggtgccggcg atgatgccga cgagcagggc gaaggcgaag tccgagaggg
      301 tcgactcgcc gaagatcagg agcgcggtga tcgggatgag ggtcgagacg gaggtgaaga
      361 tcgagcgcgt gacgacctgc cggatggagg tgttgatcat gtcgtcgaag cgccgcttgt
      421 tgtagcccac ggcgggcgtg ttctcccgga tgcggtcgaa gatgatgatg gtgtcgtaga
      481 gcgagtaacc caggatggtc agcaccgcga cgaccgtcac caggttcacc tcccgtccga
      541 cgatggcgta gaacccgatg gtcatgagta tgtcgtgcag gagcgcgacc atcgccgcca
      601 tcgcgaacga gaactcgaag cggaagctga taaacaccac tatcacgagc agcgccgccc
      661 ccaccgcctg cagggcctgg tccctgacct gccccccgaa cgtcgggctg atgctctgca
      721 cgctgatctc cgcgccgagg gccttcgcca gcgcgtcccg caccttcgag gtctccggct
      781 ggctcaggac cggcgtgcgg acctcccagc cgttgttgcc ggcccgctgg atcacggcgt
      841 cctggcggtg ctcggggggc atcgccgccc ggacctcgcc gacggtaagg tcgttcttcg
      901 ggctcgccgc gttgaacttg gcgccgccct cgaagtctat tccgaggttc aggccgcgca
      961 ccgcgatgaa cccgataccg atgagcagga agatgccgga gagcacgaac cacgtcttcc
     1021 accggccgac gaagtccacc ctgctcaacg ctttcgcgaa agccatcagc tcaccctttc
     1081 gttccgcgtc gagatactgg ccttcgagac gcccatcaag cccggcgaga ggtggatgcc
     1141 gcggctggag agcaggccca gaagcgccct cgtcaccacg atagccgtga acatcgagag
     1201 cacgacgccg ataaccagca gcaccgcgaa cccgcgaacc gagccgctgg agagcgcgaa
     1261 gaggatgaag gcgacgatga tcgtcgtcag gttgccgtcc aggatggccc ggaagccctt
     1321 ctcgtaaccg agggcgatgg ccgagcgcgg ggtcttgccg cgccggacct cttccttgat
     1381 ccgctcgaag ataacgatgt tcgcgtccgc cgccacccca atactgagca cgatccccgc
     1441 gatgcccggc aacgtcatcg tcaccgggat ggcgacgata aggccccaca acagtaacgc
     1501 gtagatgagc agagccaggt ccgccacgag cccgagggcg cggtagatga ccagcaggaa
     1561 gatcaggacc agcccgaagc ccacgagcgc cgccagcagg ccgctcttga gcgagtccgt
     1621 gccgagcgtc ggcccgatag tcgtcaccga gaggactttc atgttgatcg gcagcgcgcc
     1681 ggtgttgagc acggtcacga gctgcttcgc ttcctgctcc ggaagtccct gcggcaggcc
     1741 gttgttggag atggagatct gctggctcag cctatcctgg accacgggcg cgctctgcac
     1801 gtcctcgtcg agcgcgatgg cgaggttccc cggcttcccg gcgagcttgg cgttgatgat
     1861 cttctgcgtc agctcgctca tcttgttccc gccctcgcgg gtaagctcca gagtaacgat
     1921 gcgcttgccg gtggacgggt cggtgtccac gttcgcgccg ttctgcttga gcgcgtcgcc
     1981 cgtcagttgc gggttcttgt ccacgacgta gccgatcacg tccgtgccct tgccctgtag
     2041 agcggggctc tcctcgaaga ggatcttggt cttgtccttc ttgtaggcgg agtctttttc
     2101 gagactcttg cggatctcgg tccgggtctt gtctatctgg tcgtccggga cgctctggcg
     2161 gccgtcggta gccagcacct ggtagaagcc gagctgggcc gtgtcgccga tgatcttgag
     2221 tgtccgctcg gtgttcgtga tgccggggat ctggacgata acctggtcgt ctccctgcac
     2281 ctgcacctgc ggctccgaga cgccgaggcg gtctatgcgc tcccggatga ggttggcggc
     2341 ctgttcgatc tcgtcccgcg taaccttgcc gcccccggtc tgcgaaccct ggagctgcgc
     2401 gctcaccccg ccccggaggt ccaggccgag cttcgtggac tggccgacgg gctgccggat
     2461 aaagatcagg tacgccgcca cccccagcag ga
//
LOCUS       DASSMB010000242        23131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_835567, whole genome shotgun sequence.
ACCESSION   DASSMB010000242 DASSMB010000000
VERSION     DASSMB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 23131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23131
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835567"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2540)
                     /locus_tag="VFJ72_13920"
     CDS             complement(<1..2540)
                     /locus_tag="VFJ72_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET7480599.1"
                     /translation="MICENCGVRPASVQVFQQQGGQRVANYLCTQCARQLGLIGGGQG
                     GANPFEMLQNLIQQQNQGPGSLFDALSQEAQEAVMRAREASAGTTSTAAIGTDHLLAG
                     IVTGENVATEALGRAGADVDGMRRRFSEFRGDPGEQIYTFTPSAKRALQLSFAAARQM
                     GAGQVGSEHLLLGLLGEGDGNAYRILSEHGANDAEALRREILRLMQQRGAQASPGAGM
                     PGAMAGGPGMGGGQQGPPSKTPTLDQVSRDLTQMARDGELDPVIGRAEEIARVIRILS
                     RRTKNNPVLIGEAGVGKTAVAEGLAQRIVSEDIPESLKGKRVLALDVGGLVAGTRFRG
                     DFEERMQQMIKELQQEEKDIILFIDELHQIVGAGGAEGAVNASNMLKPALARGELQVV
                     GATTLDEYRKHVEKDPALERRFQPVLVDEPTVDETVAILFGLRDRYEAHHRVRISDEA
                     IIAAAQLGDRYITDRFLPDKAIDLLDEAAAEVRLRSTVPPVDVKRIEEEIANLEREKD
                     DAVRAEDYEKAASLKQRQEQLKVELREHQEGWVGRRETDAPEVCREDIARILEEWTGV
                     PATNIVQEEAERLLSLEAVLHERVVGQEKAVKAVAEAIRRARAGIKDPKRPVGSFIFL
                     GPTGVGKTELARTLAEYLFGEEDAMIRVDMSEYQEKHTVSRLVGAPPGYVGYDEAGQL
                     TEAVRRRPYSVVLFDEIEKAHPDVFNILLQVLDDGRLTDAQGRTVNFENTVVIMTSNV
                     GSQHLVSTKQFGFTSRDGVDFKETERRATEALERAFRPEFLNRVDEIIVFQPLTKEDV
                     LKIVDIMLKRLNKHLESQRVAVEVTPAAKELLAEEGYDPKFGGRPLARA"
     gene            2695..3663
                     /gene="pfkA"
                     /locus_tag="VFJ72_13925"
     CDS             2695..3663
                     /gene="pfkA"
                     /locus_tag="VFJ72_13925"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET7480600.1"
                     /translation="MRIAVLTSGGDAPGMNAALRAVARTGFFRGWEVMGVWDGYRGLL
                     EGRIYPMDRRRLGGIIHRGGTVLGTERSAEFATPEGQRKAAGKLAEAEIDGLIVIGGN
                     GSLTGALKLADLGVSVVGIPASIDNDIWGTDTAIGVDTALNTALEAIDRIKDTASSHR
                     RAHVVEVMGRDCGYLALMSAIAGGAEAAVVPEFEPRPEGLLRFLKMAYEQGKSHFIVV
                     VAEGAPVSAEDLHEYFNNEGTYDSRLTVVGHIQRGGSPTASDRILASRFGAAAIEALA
                     DGEVGTMVALRGEKVERVPIKDVIGKERPLDPDLYKIAEVLAELPE"
     gene            3715..4383
                     /locus_tag="VFJ72_13930"
     CDS             3715..4383
                     /locus_tag="VFJ72_13930"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7480601.1"
                     /translation="MQERGLVGLTARTAAGDEVGRITEVVTDEESGELTHVIVENQEG
                     DQFEVPITAVSLDPDADFATFHADPSDDEPGDHVGDEEEPEDYAPRDSDEEDSRHEGQ
                     LVTEPESDEEGARPEEDLAREVWEDESYTADSGYPRNDVYVDPDTGEEGTDPLLKDNQ
                     SLEDDVADLLDGTGLTVRSARDGVVELTGASSTQEDLESVIEELMGLDDVLEVDTVDV
                     DVSG"
     gene            4424..5236
                     /locus_tag="VFJ72_13935"
     CDS             4424..5236
                     /locus_tag="VFJ72_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7480602.1"
                     /translation="MTTPQQRVRRGPKSSLGWLEHFRANEEPEVLTREGPRLSEAEKA
                     AVVASVQEFQLGESSEGRHLHRLAAEHARRTDDEDYLRAIEYFIREEQRHARYLRDFL
                     ALEGFGTIKHRWTDTVFRRLRNLAGLEVSIGVLLTAEVIAKVYYEGLKGATRSLVLRR
                     ICERILRDEEAHVRFQSERLAVLRRGRPEPGRKLTGLPHRTLFFGACLVVWKNHGEAM
                     RRGGMDFRGYWLRCWEEFGTAAGMMAPESYTFPAHGKEGLSPRRMTDDGPYL"
     gene            complement(5195..5614)
                     /gene="queF"
                     /locus_tag="VFJ72_13940"
     CDS             complement(5195..5614)
                     /gene="queF"
                     /locus_tag="VFJ72_13940"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HET7480603.1"
                     /translation="MDLRGEEKTVQENPVLGRETRGPIGAEGLDTVPWNHADTDAVVE
                     FTTNELTAMCPVTGQPDFYDLKLSYRPKEKLLESKAMKLYLWGFREKGMFAEDMAATL
                     LKDLVAACDPVEMTVDLTQQVRGGLEIRTVVRHPSRG"
     gene            complement(5648..6391)
                     /locus_tag="VFJ72_13945"
     CDS             complement(5648..6391)
                     /locus_tag="VFJ72_13945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7480604.1"
                     /translation="MDNTLRGRAAVVTGASSGIGAAVARALNAEGARVALAARREDAL
                     HEVRSSLGGGAESLAVPTDVTDRAAVKALVERAGAELGPVDILVNCAGVMYYTLMKNL
                     REDEWERTVEVNCKGALNCAGAVLPGMLERGRGHIVTISSDAGRKVFPGLAVYSASKF
                     FVEALSQGLRLETAGTGLKVTTIQPGNVATDLLSMSSDPEALKLYGEPTGARVLDPED
                     VAASVIHALKQPDHVAVNEVLVEPRDEPV"
     gene            complement(6384..9482)
                     /locus_tag="VFJ72_13950"
     CDS             complement(6384..9482)
                     /locus_tag="VFJ72_13950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HET7480605.1"
                     /translation="MSGNPTDRCLHEIFEEQARRTPEALALVDQHTSLTYAELDRRAD
                     LLAIRLRRSGVGPGELVGVYMERRAEYVVACLAALKSGGAYLTLELAYPPSLLEEVIS
                     DADPRIVLTRESYTERLPDGVERFCLDEGWETGLEEALEDAPAEDARVSQENLAFVSY
                     SSGTTGKPKGIANPHRAPVRSYLWRFGVSDYSPGDRVGCNVFFIWEMLRPLLRGAATV
                     VIPDDVIYDPNALVRFLREYEVTETLVTPSLLEAVLDASGPEIGDRLPALRTVWLNGE
                     VVTRTLARRAMSLLPDTRFLNVYSCSETHEVAAGDLRELVENPDSTYCPVGLPMDPER
                     LYVLDEEGRTVPDGEAGELFVGGECLARGYVNLPEKTEESFPEDPFSSVSGARMYRSG
                     DRARILSDGSLEIMGRVDFMVKIRGYSVELGAVEAAIEKDLAVRTCVAIAEGGEGEDK
                     RLVAYLVPDPEDGERYSGWDLDPKTGRSRGIRAALRDSLPHYAVPAVFVELESLPIQA
                     TSGKVDRDELPPPPPRPSRAAPDPHRSAMELPADAPRSEKEALLERVWEEVLRLDEGE
                     VRPEDDFFDLGGHSLAAAQLSGRVEESFGVHVSMPLFMEDPTVGGLCDRIESLQRGGS
                     NDAARISADDLRAEAVLDPEIIPERADAGGAKTLRDARNVFLTGATGFLGAFLLDGLL
                     SSTEARVHCLIRPRGDEDSLETIRANLRDYGLDGAARSERVVAVPGDLGEPLLGIDER
                     DFDALARETDVVIHAGAVVNLVYPYSVLKAANVGGTREVLRLACRHRVKPVHYVSTNG
                     IFPSGRGLCEEDADLDALAGGREDGYGQSKWVAERLVREAAERGVPVSVYRPGNISGH
                     SASGSSNPRDLLGAVVVESLRLGAAPEVEGWRMEMTPVDFVAAAILHIASDSEAVDGN
                     YHLANPDPIHASEFFDRLEELGYPLERLPYPEWLDAVKDVPHRDDDVTGGVLRGASPE
                     DDEIWDGNIYDDRNTREALRGGGPQRPVFDTELIETYARYFVEQGWVEAPAALQEANR
                     RG"
     gene            complement(9823..10014)
                     /locus_tag="VFJ72_13955"
     CDS             complement(9823..10014)
                     /locus_tag="VFJ72_13955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480606.1"
                     /translation="MIITYASLGALFMPFLAITLMWLLNRRVDREYRSGLLSNIILGA
                     SVLLFAVLAVREILGQFGG"
     gene            complement(10011..11120)
                     /locus_tag="VFJ72_13960"
     CDS             complement(10011..11120)
                     /locus_tag="VFJ72_13960"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7480607.1"
                     /translation="MTDVTGGAGRESVERREAPSTWGSRLKLVGPGLVVAATGVGAGD
                     MVSSLTAGTEYGTVLIWAIILGAALKFALTEGLGRWYMATKTTVLDGWHSMGWWATGY
                     FLVYLALVTFFFGAAAPSASALAVMAMFPGVMPLWAWAVLHSVVFGFLICIIGKYGLF
                     EKVMEAFVGIMFLTVVGLALFLGPNIGELALGTVVPRIPEGSLPYALAVIGGVGGTFT
                     LVSYTYWVRERGWRRPSWIPMMRTDLAVGYIMTGLFMVAMLVIGAELLFAGGKSIADE
                     GGLVALSDPIARRFGVVPSWLFLIGFWAAATSSITGAWNGGAYLFGDLIRTIRRVPEE
                     QGEEYLSEKSVWFRAFLVWVTFPPRSFSPSMSRCG"
     gene            complement(11210..11680)
                     /locus_tag="VFJ72_13965"
     CDS             complement(11210..11680)
                     /locus_tag="VFJ72_13965"
                     /inference="COORDINATES: protein motif:HMM:NF017941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET7480608.1"
                     /translation="MLKGSPARRVAIAGVVGALYVVLSLLVAPLAYGPIQFRLGEVLK
                     PLVIKYPATIPAFAVGVGIVNLFSPQAGGLELILMPIVNLVGGALCWVLARRIGGVFA
                     TYLASLVFALIIAAGVATVLHFAAGLPYLISFGSVAVSEIILLLLGNTVLVRRV"
     gene            complement(11697..13319)
                     /gene="ggt"
                     /locus_tag="VFJ72_13970"
     CDS             complement(11697..13319)
                     /gene="ggt"
                     /locus_tag="VFJ72_13970"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET7480609.1"
                     /translation="MFRSEEAATSVRRPDQGGRSTVFGTKGAVACEHPSAALAGIRAL
                     DAGGTAADACVAMAAAMAVLAPMTTGMGGDAFLLFYEAGTGRVLGANGSGRAPRGATI
                     EKLRDRGIAEIPERGGLSITVPGAVRLWEDAADRLGRLPLADLLEPARDFAEEGRPVA
                     EVVARYWEVAEALLRRNEAAASTFLPDGRAPRPGDLFRQPDLAETLAAVAEEGADAFY
                     KGRIARSITDAAQEAGGYLAGEDLAAHETTWVEPIHTDYRGVRVYEIPPPGQGIAALE
                     MLNILEGFDMSALDPLGAERIHLEVEAKKLAFADLHAKVGDPEFAGVPTEELLSKDYA
                     AKLRGRISRDRVGVSVGPALGDDTTYLCAVDAEGNGCSFINSLYMGFGSGVVAGGTGV
                     CLQNRGHSFRLDPDHPNVLVPGKRPLHTIIPGLATCDGALWSVFGNMGGPMQPQGHFQ
                     VLSNMIDHGMNPQETVDHPRHFHDHNSNVLLVEGRVPEAEVEELRSLGHEVEVGEDYI
                     VPVGGAQIIRVMESGARACGSDPRKDGCALAQ"
     gene            13418..13573
                     /locus_tag="VFJ72_13975"
     CDS             13418..13573
                     /locus_tag="VFJ72_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480610.1"
                     /translation="MGKGAYVGGTLVSILISLALYFSGRREAGIFVGLWAPTILNLGQ
                     TLIDDES"
     gene            complement(13684..14019)
                     /locus_tag="VFJ72_13980"
     CDS             complement(13684..14019)
                     /locus_tag="VFJ72_13980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480611.1"
                     /translation="MVAFEDEYRAYQEALASALRMLRPHAEVSTDRPEDETSASARLR
                     PDLAIRNRADPGEGLAWLELSMEPGLPSRVRIGEQSSTVVNPSLEEILYIVDEVERLT
                     RATTGPEDE"
     gene            complement(14095..14505)
                     /locus_tag="VFJ72_13985"
     CDS             complement(14095..14505)
                     /locus_tag="VFJ72_13985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480612.1"
                     /translation="MTKTKTRRLLLIEDHLWFRQALALFLDGEPDLHVVAQAGTLDEC
                     HGELVDDGLEDVDVAIVDLTLPDGDGIDLIHWLREYAPHIAVLVLTVSLETERHDLAL
                     GAGASEVISKSDSIENILDAVRRLADSPAIPRSA"
     gene            complement(14595..15146)
                     /locus_tag="VFJ72_13990"
     CDS             complement(14595..15146)
                     /locus_tag="VFJ72_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480613.1"
                     /translation="MATPITDAADLPDGGFETHQTFAEDGRGAAIMTRSLADRVIEAA
                     NVLFGSYSSKAARLDRQADEMTTSLLRAAAGLIGSLAGKHERSAPAQPSRNAPAPAAP
                     ASPAPGPASVPMPAPFPVPAGGGTSCSSSSGSWQCHNGSTGLMLFVLAGMLALGVPRG
                     STRFFQDVLKPNSIIPQVAERPG"
     gene            complement(16053..17642)
                     /gene="mutL"
                     /locus_tag="VFJ72_13995"
     CDS             complement(16053..17642)
                     /gene="mutL"
                     /locus_tag="VFJ72_13995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET7480614.1"
                     /translation="MKLFPRLIAEELAEYGGKVHVLPPEVAHRIAAGEVIERPASAVK
                     ELVENSLDAGASRVEVDIEGGGVSLIRVRDDGSGMSPEDAERAVAEHATSKIETADDL
                     ARVVSLGFRGEALHAIGSVSLLALTTCERGAARGTRVRVLAGETVEAGPAAHPSGTTV
                     EARDLFLNLPVRRGFLGTARAEANAVATVVQALALSRPEAGFFLRSDGRGVLALPAAA
                     DLRERISQAHGLSLARSLVELEDPVVWGFVSPLSVSFPTRRYLHVAVNGRVVDTDSFA
                     PAVAKAYADLLPKGRHPAAFLRLDLGPGEVDVNVHPAKSAVRLRGGRSAYPLVVGTIR
                     AALAVEREVVGGAAPDERNTELKLIGQFAGRCIVAQEGEELIILDQHGAHERILYERL
                     SENPKGEAMKLPDPVVARLPEDLAPEIWGLEAELNSIGFRFEPFGADAVRVLAAPDLV
                     TDPEVALLAAVEALAGGEDLAKALACKGSTKFGEELSVEEMRALLADWSDCEFGEVCP
                     HGRPIVRRIALADLLRDFGRA"
     gene            complement(17639..19999)
                     /gene="mutS"
                     /locus_tag="VFJ72_14000"
     CDS             complement(17639..19999)
                     /gene="mutS"
                     /locus_tag="VFJ72_14000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET7480615.1"
                     /translation="MLGRHAELKAQLPPGTILFYQVGTFFETFEEDAKKISQALSIRL
                     TSREAAGYDGGRVPLAGVPGHALQEHLATLLRKGYSVAVAEQRPHPTKPKQFTREVTR
                     ILTPGTVIEDNVLSAGEANYLAAFVVRDGQAGIAVVEASTGEFTGTEVPEEQLAAELE
                     RWQPRELVVPERTAAENLPKVRATVSSAPRWTFEPSAGEQALKRHFQVSGLKGFGLDG
                     RPALVGAAGALLQYLASLRGGHAPEQVVTFRPYDPGSGMVLDAATRRNLGLDDLIETI
                     DRTRTPMGQRALRRWLERPLLEVDRIEQRLAAVDALVPDYMLREEVRELLSRIPDIER
                     IATRIVRLSASPNDLLSLKGALEAIGPLKEVLAPATERSSLLSRVLAAMEEPPGVREL
                     ISAAISEESGEVIRAGYSSELDEAREFRDGAHDWLTRFEADERLKTGLKSLKVGYRDG
                     EGYFIEVAGKDTGAVPDRYEHRKALKHNARFVTVELKEHESRMLTARDEVERLERRIL
                     SEIRAAVKEAAPELQKIARAVAVVDVVASFAAAATELRYCRPAVTQRRGIRVIGGRHP
                     VVEHNTGTPFVPNDSEVYGDSRLQIITGPNMAGKSVYLRQVAIITLLAQTGSYVPADE
                     ATLGVVDRIFTRVGAEDRLASGESTFMVEMTEAASILNGATERSLVILDEVGRGTSTY
                     DGMSLAWAIAEYLHDDVQSLTLFATHYHELTRLAEILPGCRNYKASVEELGGEIVFLH
                     RIEAGAESSSYGVHVARLAGLPRQVTDRAGEILTRLEAEGVKDFAG"
     gene            20076..20777
                     /locus_tag="VFJ72_14005"
     CDS             20076..20777
                     /locus_tag="VFJ72_14005"
                     /inference="COORDINATES: protein motif:HMM:NF016155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HET7480616.1"
                     /translation="MYDADPGLSKVILAHAALGAALNLAFSGRDRGLRRALVLFALGA
                     GLPALGELLVTAPLGLLRHRTRPRVAGVPLGVLLGWYCAIHGSFTVTARILARLPIDE
                     DRRHAALPFGAALVGTSLDLILDPFGLDAGLWEWTTDGVYAPEVVGANGRSGVPLINY
                     LGWISLVASVVYVHKRLFEDGTSPDLDASRIPGLLLLPYYLAAAVWAVRRRRLRYLVY
                     SVIFPVALGVSLRGR"
     gene            complement(20788..21702)
                     /locus_tag="VFJ72_14010"
     CDS             complement(20788..21702)
                     /locus_tag="VFJ72_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7480617.1"
                     /translation="MGYFPTKILLATDGSEDAAVARRAATDLAGRSGSELHVVHAFEF
                     IPPREYMSVALRLRSPEFTCHGQRLLDEQVERIEESGGVVAEARLMMGSPVDQILTAS
                     EEIGAGLLIVGRRGLGGVKRLLMGSVSEGVVHHTRCPVLVLRCEDDAWPPSRIIMADD
                     SSDDAGKSAELAASIGALFGARGSLVQVYPRLLKSSQTHSPLEVRMVEQALKQAEAEL
                     RERAEDLERILGSRPDVKLMVDEDADGIDGIALTLLDTASETGEPTMISVGSRGLGRI
                     QRSRVGSVSTKVVRAADGPVLIYPHSQD"
     gene            complement(21724..22737)
                     /locus_tag="VFJ72_14015"
     CDS             complement(21724..22737)
                     /locus_tag="VFJ72_14015"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480618.1"
                     /translation="MYTIIEFVLALVVILLGAAFFTNAVELLGGRLGMQQGAVGSLLA
                     AVGTALPESMIAVVAILQPVISGDTSGDGASIGIGAILGAPFMLATLALFVVGASVLV
                     FRRRRSQGSAVSVDNATIGRDIGFFLVFFAIAAGVGMVDLPLVLKVVVALALVAGYAW
                     YAKLTLSSGGHLENVPERLLFLPRTQNPPTFAIAAQALASLGAIVAGAHFFVDAVEHA
                     AAGLGLPAGLIALVLAPLATELPEKFNSIFWVRDGKDTLAMGNVTGAMIFQSTIPVTF
                     GILFTEWDLTGLDLFAVALALVSAGIAYLVLKSSGKLRAGHLLIGGVFYAAFVVGAVV
                     AVL"
     gene            complement(22775..>23131)
                     /locus_tag="VFJ72_14020"
     CDS             complement(22775..>23131)
                     /locus_tag="VFJ72_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480619.1"
                     /translation="IVMIGTTDVVFAVDSIPAIFSITSSAFVVWSANAFAVLGLRPLY
                     FMLAGMIDRFVYLSLGLSIILIFVGAKFFYSGIFDAKVPIWVSLPFIAVVVAASIAAS
                     LWKTRNGRTPPEETRQ"
BASE COUNT         3885 a         7984 c         7482 g         3780 t
ORIGIN      
        1 gcgcgggcga gtgggcgtcc gccgaacttg gggtcgtaac cctcttccgc cagaagctcc
       61 ttcgcagccg gtgttacctc tacggcgacg cgctggcttt cgaggtgctt gttcaggcgc
      121 ttcagcatga tgtccacgat cttgagcacg tcctccttcg tgagcggctg gaagacgatg
      181 atctcatcaa cgcggttcaa gaactccggc ctgaatgccc gctccagcgc ctctgtcgcc
      241 cgccgctcgg tctccttgaa gtccacgccg tcgcgcgagg tgaacccgaa ctgcttcgtc
      301 gagacgaggt gctggctccc gacgttcgag gtcatgataa ccaccgtgtt ctcgaagttg
      361 accgtgcggc cctgcgcgtc cgtcaggcgg ccgtcgtcga gcacctgcaa gaggatgttg
      421 aacacgtccg ggtgagcctt ctcgatctcg tcaaagagaa ccacgctgta cgggcggcgg
      481 cgaaccgctt ccgtcaactg tccggcctcg tcgtagccga cgtagcccgg aggcgccccg
      541 acgagccggg agacggtgtg cttctcctgg tactcggaca tgtccacccg gatcatggca
      601 tcctcctcgc cgaagaggta ctcggcgaga gtgcgcgcca gctccgtctt gccgactccg
      661 gtaggaccga ggaagatgaa gctcccgacc ggccgcttcg ggtcctttat accggcccgc
      721 gcgcggcgta tagcctcggc gacggccttt acggccttct cctggcccac gacccgctcg
      781 tgcagcacgg cctccaggct caggagccgc tcggcctcct cctggactat gttggtggcg
      841 ggtacgccgg tccactcctc caggatgcgg gcgatatcct cgcggcagac ctcgggagcg
      901 tcggtctcac ggcgtccgac ccagccctcc tggtgctcgc gcaactcgac cttgagttgc
      961 tcctggcgct gcttgaggct cgcggccttc tcgtagtcct cggcccgcac ggcgtcgtcc
     1021 ttctcgcgct ccaggttcgc tatctcctcc tctatgcgct tcacgtcaac cggcgggacc
     1081 gtagagcgca gccgcacctc ggccgccgcc tcgtccagca ggtctatcgc cttgtccggc
     1141 aggaagcggt cggttatgta gcggtcgccg agctgggcgg cggcgattat ggcctcgtcc
     1201 gagatcctca cccgatggtg cgcctcgtag cggtcgcgca gcccgaagag tatggctacc
     1261 gtctcgtcca cggtcggctc gtccaccagc accggctgga agcggcgctc aagcgccggg
     1321 tccttctcga cgtgcttgcg gtactcgtcc agcgtcgtcg cgccgaccac ctgaagctcg
     1381 ccgcgcgcga gggcgggctt gagcatgttc gaggcgttca ccgcgccctc ggcgccgccg
     1441 gcgcccacga tctggtgcag ctcatcgata aacaggatga tgtccttctc ctcctgctgc
     1501 agctccttga tcatctgctg catccgctcc tcgaagtccc cccggaagcg cgtgccggcc
     1561 acgagaccgc ccacgtcgag cgccaggaca cgcttgccct tgagcgactc cgggatgtcc
     1621 tcggagacta tgcgctgcgc gagcccctcg gccacggcgg tcttgcctac gccggcctca
     1681 cctatgagga cggggttgtt cttggtgcgc ctggacagga tgcggatgac gcgggctatc
     1741 tcctcggcgc ggccgatgac cgggtcgagc tcgccgtcgc gggccatctg ggtgaggtcc
     1801 ctactgacct gatccagggt cggcgtcttg ctcggcggac cctgctgacc accacccatt
     1861 ccgggacctc cggccatcgc gcccggcatt ccggcgccgg gactcgcctg ggcgcctcgc
     1921 tgctgcatga ggcgcaggat ctcacgtctc agcgcctcgg cgtcgttggc gccgtgctcc
     1981 gagagtatcc ggtatgcgtt cccgtccccc tcgccgagca gcccgagcaa caaatgctcg
     2041 ctgccgacct gccccgcgcc catctgccgg gcggcggcga aagagagctg caaggcccgc
     2101 ttggcgctcg gcgtgaacgt gtagatctgc tcgccagggt ccccccggaa ctcggagaaa
     2161 cgacgccgca tcccgtcaac gtccgcgccg gccctgccaa gcgcctcggt cgccacattc
     2221 tcgccggtca ctatgcccgc gagcaggtgg tcggtcccga tggccgccgt gctggtcgtc
     2281 ccggccgagg cctcgcgggc gcgcatcacg gcctcctgcg cctcctggga gagcgcgtcg
     2341 aagaggctcc ccggcccctg gttctgctgc tgtatcaggt tctgaagcat ctcgaacgga
     2401 ttggctccgc cctgcccgcc cccgatcagg ccgagctgcc gcgcgcactg cgtgcacagg
     2461 tagttcgcaa ctcgctgccc gccctgctgc tgaaacacct gcacactggc cggccgcacc
     2521 ccgcaattct cacagatcat ctctcggaat ctcccatctg tctgaaatct aagtcttcaa
     2581 cgctcatatt ttattaccgg aaactgatag atcaagagtg atagatcaag agcagtatcc
     2641 gggggccgcg cgttcgtata attggcgttc aggacggagc gagaggagac ggagttgagg
     2701 atagcggtat tgacgagcgg gggcgacgct ccggggatga acgcggcgct gcgggcggtg
     2761 gcacgtacgg ggttcttccg gggatgggag gtcatggggg tatgggacgg ctacaggggg
     2821 cttctggaag gcaggatcta cccgatggac cggcgacggc tcggagggat catccaccgg
     2881 ggcgggacgg tgctcgggac ggagcgctcg gcggagttcg ccacccccga ggggcagcgc
     2941 aaggcggcgg gaaagctggc ggaggccgag atagatggtc tgatcgtcat cggtggcaac
     3001 ggtagcctga cgggcgctct caagctcgcg gacctcgggg tatccgtggt gggcatcccg
     3061 gcctccatag acaacgacat atggggcacg gacacggcca tcggggtgga cacggcgctc
     3121 aacacggccc tcgaagccat agaccgcata aaagacacgg cgagcagtca ccgccgggcg
     3181 cacgtggtcg aggtcatggg gcgcgactgc gggtatctgg cgctgatgag cgcgatcgcg
     3241 ggcggcgcgg aggcggccgt ggtccccgag ttcgagcccc ggccggaggg cttgctgcgc
     3301 ttcctcaaga tggcatacga gcaggggaag tcgcacttca tcgtcgtggt ggcggagggg
     3361 gcgccggtct cggcggagga cctacacgag tacttcaaca acgagggcac ctacgactcc
     3421 cggctcacgg tcgtggggca catccagcgc ggcggcagtc ccacggcctc ggaccggata
     3481 ctggcgagcc ggttcggcgc ggcggcgata gaggcgctcg ccgacggcga agtcgggacg
     3541 atggtagcgc tgagaggaga gaaggtcgag cgcgtcccga taaaggacgt catcgggaag
     3601 gagcgtccac tggaccccga cctctacaag atcgccgagg tgttggcaga actgccggag
     3661 tagcgtcatc ggggtatatt tcctcaacga tccagaacga gcgaggaggc cagcttgcag
     3721 gagcgcggac ttgtaggact tacggcgcgg acagcggcgg gcgacgaggt cggccgcatc
     3781 acggaggtcg tcaccgacga ggagagcggc gagctgacgc acgtaatcgt ggagaatcag
     3841 gaaggggatc agttcgaggt tccgatcacg gccgtctcac tcgacccgga cgcggacttc
     3901 gccaccttcc acgccgaccc ctcggacgac gaacccggcg accatgtagg tgacgaagaa
     3961 gagccggaag attacgcgcc ccgcgactcg gacgaagaag actcgcgcca cgagggtcag
     4021 ctagtcaccg agcccgagag cgacgaggaa ggagcgcgac cggaggaaga cctcgcgcgc
     4081 gaggtctggg aagacgagtc ctacaccgcc gattccggct acccgcgcaa cgacgtctac
     4141 gtcgacccgg atacgggcga ggaagggaca gaccctctcc tgaaagacaa ccagagtctg
     4201 gaagacgacg tcgctgacct cctcgacggc acgggcctta cagtacgctc cgccagagac
     4261 ggcgtcgtgg agcttacggg ggcttcgtcc acgcaggagg acctggagag cgttatcgag
     4321 gagcttatgg gtctcgacga tgtccttgag gtggataccg tcgacgtaga cgtctccggt
     4381 tagcccaggg cgttgtgagc cgacgccggg ttagacccgc gccgtgacca cgccgcaaca
     4441 gagagtgcgc cgcggcccga aaagttccct gggctggctg gaacacttcc gcgccaacga
     4501 ggagcccgag gtcctgacgc gagaagggcc ccggctctcg gaggcggaga aggcggcggt
     4561 ggtcgcatcg gtgcaggagt tccagctcgg cgagagttcg gagggcaggc atctgcaccg
     4621 gctcgccgcc gagcacgcgc ggcggacgga cgacgaagac tacctgcgag ccatcgagta
     4681 cttcatccgc gaagagcagc ggcacgctcg ctacctgcgg gacttcctcg ccctggaagg
     4741 tttcgggacg atcaaacatc ggtggaccga cacagtcttc aggaggctgc ggaacctggc
     4801 cggactggag gtctccatcg gagtgttgct cacggcggag gtcatcgcca aggtctacta
     4861 cgaaggactg aagggagcca cccggtcgct ggtcctgcgg aggatctgtg agcgcatact
     4921 ccgggacgaa gaggcgcacg tccgctttca gtccgagcgg ctggcggtcc tccgccgggg
     4981 acgcccggag ccgggtagaa agctcacggg tttgccgcac cgcacgctgt tcttcggcgc
     5041 ttgcctcgtg gtgtggaaga accacggcga agccatgagg cgcggcggga tggactttcg
     5101 gggctactgg ctccggtgct gggaggagtt tggaaccgca gccgggatga tggccccgga
     5161 gagctacacg ttcccggcgc acggcaagga aggcttatcc ccgcgaagga tgacggacga
     5221 cggtccgtat ctctaacccg ccccgtacct gctgcgtcag gtcaaccgtc atctcgaccg
     5281 ggtcgcaggc cgcgacaagg tccttgagta gcgtggccgc catgtcctcc gcgaacatgc
     5341 ccttctcccg gaagccccac aggtagagct tcatcgcctt agattcgagc agtttctctt
     5401 tcggacggta gctcaacttc aggtcgtaga agtccggctg accggttacg gggcacatcg
     5461 cggtcaactc gttcgtcgtg aactcgacga ccgcatccgt gtccgcgtgg ttccagggga
     5521 ccgtgtcgag tccctccgcc ccgatagggc ctcgcgtctc gcggcccaga actggattct
     5581 cttgcaccgt cttctcctcg cctctgagat ccatgatgtt gctatgttac cgcagccggg
     5641 atccggttca gacaggctcg tcgcgcggct cgaccaggac ctcgttgacg gcgacgtgat
     5701 ccggctgctt aagagcatgg atcacggacg cggcgacgtc ctccgggtcc agcacccgcg
     5761 ctccggttgg ctctccgtag agcttgagcg cctccgggtc gctgctcatc gagagcaggt
     5821 ccgtagccac gttgcccggc tggatggtgg tgaccttcag ccccgtcccg gccgtttcga
     5881 gccgcagccc ctgggagagc gcctcgacga agaacttgct agccgagtag acggcgagtc
     5941 cggggaatac tttcctgcca gcgtcggagg agatggtgac gatgtgtccc cggccgcgct
     6001 ccagcatccc cggcagcacc gctccggcgc agttgagagc gcccttgcag ttgacctcga
     6061 ccgtccgctc ccactcgtcc tcgcgcaggt tcttcatcag cgtgtagtac atgacccccg
     6121 cgcagttgac gaggatgtcc accggaccca actccgcccc ggcgcgctcc acgagcgcct
     6181 tcaccgccgc ccggtccgta acgtccgtgg gaaccgccag gctttcggcc ccgcccccca
     6241 gcgaggaccg aacctcatgc agcgcgtcct cccggcgggc ggcgagcgcg acgcgagcgc
     6301 cctcggcgtt tagcgcgcgg gcgacggccg cgccgatgcc gctcgaagcc ccggtgacca
     6361 ccgcggcccg gccccggaga gtgttatcca cgccggttcg cctcctgtag agccgcgggc
     6421 gcctccaccc atccttgctc cacgaagtac cgggcgtagg tctcgatcag ctccgtatcg
     6481 aagaccggcc gctgtgggcc gccgccacgc agagcctccc gcgtattgcg gtcgtcatag
     6541 atattgccgt cccagatctc gtcatcctca ggcgaggccc cacgcaggac gccgccggtg
     6601 acgtcgtcat cccggtgcgg cacatctttg accgcgtcca gccactcggg gtatgggagt
     6661 cgttccagcg ggtagccaag ctcctccagc cggtcgaaga actcggaggc atggataggg
     6721 tccgggttcg ccaggtggta gttgccgtct acagcctccg agtcggaggc gatgtgcagg
     6781 atcgcggcgg ccacgaagtc caccggcgtc atctccatcc gccatccctc gacctccggc
     6841 gcggccccga gcctcagtga ctccacgacg acagcgccga gcagatcgcg cgggttcgac
     6901 gagcccgacg cgctgtggcc cgagatgttc cccggccggt acacgctcac cggcacaccg
     6961 cgctccgccg cctcccgcac gagcctctcc gcgacccact tggactggcc gtagccatcc
     7021 tcgcggccac cggcgagagc atccaggtcc gcgtcttcct cgcagagacc cctcccggac
     7081 gggaagatgc cgttggtgga gacgtaatgc acgggcttta cccggtggcg gcaggccagg
     7141 cgcaagacct cgcgggtccc gcccacgttg gcggctttca ggacggagta cgggtagact
     7201 aggttcacga ccgcgccggc gtggatcacc acgtccgtct cacgcgccag agcgtcgaag
     7261 tctctctcgt caatacctaa cagcggctcg ccgaggtcgc ccggcacagc cacgacgcgc
     7321 tccgagcgag cggcgccatc cagaccataa tcccgcaggt tcgcccggat ggtctccagg
     7381 gagtcctcgt ctcctcgcgg acggatgaga cagtggacgc gagcctccgt gctggagagc
     7441 agcccgtcga ggaggaacgc ccccaggaag ccggtcgcgc cggtgaggaa gacgttccgc
     7501 gcgtcgcgca gagtcttagc gccgcccgcg tccgcgcgct cggggatgat ctccgggtcg
     7561 agaacggctt cggcgcgcag gtcgtcagcc gagatccggg ccgcatcgtt cgaaccgccg
     7621 cgttgcagcg actcgatcct gtcgcacagc ccgccgacgg tcgggtcttc catgaacagc
     7681 ggcatcgaga cgtggacgcc gaagctctcc tccacgcgac cggagagctg ggcggcggcg
     7741 agcgagtgcc cgccgaggtc gaagaagtcg tcctcgggac gcacctcgcc ctcgtccagt
     7801 cggagcacct cctcccacac ccgctcgagc agggcttcct tctcggagcg cggagcatcg
     7861 gcgggaagct ccattgcgct ccgatgcggg tccggggcgg cgcgggaggg ccggggcggc
     7921 ggtggcggca gctcgtcgcg gtccaccttg ccggaggtcg cctggatcgg taacgactca
     7981 agctccacga agaccgccgg gaccgcgtag tgcggcaggc tgtcgcgcag cgcggcccgg
     8041 atcccccggc tccgcccggt cttcgggtcg aggtcccatc ccgagtagcg ctccccgtcc
     8101 tccgggtccg gcaccaggta agcaacgagg cgcttgtcct cgccctcgcc gccctcggcg
     8161 attgcgacgc acgtcctcac cgcgagatcc ttctcgatgg ccgcctccac cgcgcccagc
     8221 tcgacgctgt aaccccgaat cttgaccatg aagtccaccc ggcccatgat ctccaggctc
     8281 ccatccgaga gtatgcgcgc cctgtccccc gaccggtaca tccgggcgcc gctcacggac
     8341 gagaatgggt cctccgggaa gctctcctcc gtcttctccg gcaaattcac gtagccgcgc
     8401 gccaggcact cgcccccgac gaacaactcc ccagcctcgc cgtcggggac ggtccggcct
     8461 tcttcgtcca ggacgtacag gcgctccggg tccatgggca gtccgacggg acagtaggtc
     8521 gagtccgggt tctcgacgag ttcccgcagg tcaccggcgg cgacctcgtg tgtctcgctg
     8581 caactgtaga cgttcaggaa tcgagtatcc ggcaggaggc tcatggcgcg ccgcgcgagc
     8641 gtccgggtga cgacctcgcc gttcagccac accgtccgca gggccgggag ccggtccccg
     8701 atctccggcc cgctcgcatc gagcacggct tcgagcaggg acggagtgac aagcgtctcc
     8761 gtgacctcgt actcccgcag gaaacggacc agggcgttcg gatcgtagat cacgtcgtcc
     8821 gggatgacga cggtagccgc gccccgcaag agaggacgca acatctccca gataaagaac
     8881 acgttgcacc cgacccggtc gccggggctg tagtcgctca cgccgaaccg ccacaggtaa
     8941 gagcgtaccg gcgcccggtg cgggttcgct atacccttcg gcttgcccgt ggtccccgac
     9001 gaatacgaca cgaacgccag gttctcctgg ctcacccgcg cgtcctccgc cggagcatcc
     9061 tccagagcct cctccagccc ggtctcccat ccctcgtcca gacagaaacg ctcgactcca
     9121 tccggcagac gctccgtgta gctctcccgg gtgaggacga tgcgtgggtc ggcgtcggag
     9181 atcacctcct ccagcagcga gggcggatag gccagttcca gcgtgaggta cgcgccgccg
     9241 gatttcaggg cggccaggca cgcgacgacg tactcggcgc gccgctccat atagacaccg
     9301 accaactcgc cggggccgac tcccgaacgc cggaggcgga tcgccagcag gtccgcccgg
     9361 cggtccagtt ccgcgtaggt cagggaggtg tgctggtcca ccagggccag ggcctcgggg
     9421 gtacggcggg cctgttcctc gaagatctcg tgcagacagc ggtcagtcgg gtttccgctc
     9481 acaccagcac ccggcctgcg gatagactcg tgttcatcgt tcgtcctgcg gagacttgca
     9541 tgatcagact ccccttctct aatacttgtc atcaacaacg ctcgacgctc taaaacaaac
     9601 caggaagcaa caggacgctg ccacagagat cctgccgggc cttgctccgg cgaatcccgg
     9661 aagcgaagca gccccgatag cattgccgga catactaccc ggcttgcgtc ctcctggacg
     9721 gggttatttc gaagatatct ttaagatcgc gttaagtttc gtgaacacgc tgccccgcac
     9781 aaatcgggag cggcgcatct catggagcct gtccgggagc ggttatcctc cgaactgtcc
     9841 gagtatctcc cgcacggcga gcacggcgaa gagcagcacg gaggcgccaa ggattatgtt
     9901 cgagaggagg ccgctgcggt attcgcggtc cacgcggcgg ttcaggagcc acatcagcgt
     9961 gatcgccagg aacggcatga acagcgcgcc cagggaggcg taggtgatga tcacccacac
    10021 cggctcatcg agggtgaaaa ggatcttggg gggaacgtaa cccagacgag gaatgcccgg
    10081 aaccacacgc tcttctcgga gaggtactcc tcgccctgtt cctccggcac tctgcggatc
    10141 gtccggatca agtctccgaa caggtaggcg ccgccgttcc acgctccggt gatggagctt
    10201 gtcgccgccg cccagaagcc gatgaggaag agccagctag gcaccacccc aaaacgccgg
    10261 gctatggggt ccgagagagc gacgagcccg ccctcgtcgg cgatgctctt gccaccggcg
    10321 aacagcaact cggccccgat gaccagcatc gccaccatga agaggcccgt catgatgtac
    10381 ccgacggcga ggtcggtacg catcatcggg atccaggacg gccgccgcca gccgcgctcg
    10441 cgcacccagt acgtatacga gacgagcgtg aacgtgccgc ccacgccccc gatgaccgcc
    10501 agagcgtagg gtaaagaacc ttccggtatc cgcggaacga ccgtccccag ggcaagctcg
    10561 ccgatgttgg gcccgaggaa cagcgccagc cccacgaccg tgaggaacat gataccgacg
    10621 aatgcctcca tcaccttctc gaagagcccg tacttgccga taatgcagat caggaacccg
    10681 aacaccacgg agtggaggac cgcccacgcc catagcggca tgacgccggg aaacatcgcc
    10741 atgacggcca gcgccgaggc cgagggagcc gccgccccga agaagaacgt gaccagcgcc
    10801 agatagacca ggaagtaccc ggtcgcccac cagcccatcg agtgccatcc atccaggacg
    10861 gtcgtcttcg tcgccatata ccagcgcccg agtccctcgg tgagagcgaa cttcagggcc
    10921 gcgccgagga tgatcgccca gatcaaaacc gtcccgtact cggtccccgc cgtgagcgag
    10981 gagaccatgt cgccggcccc cacgccggtc gccgcaacaa cgagccccgg ccccacgagc
    11041 ttcaggcggc tcccccacgt cgaaggagcc tccctcctct cgacggactc ccgtccggcg
    11101 cctccggtaa cgtcagtcat ccgtccccct tcccaagcct ccaatcgtta cattggacat
    11161 taaattagcc tggaagacaa atggaagccg gtaagctggc tggtgttgtt cagaccctcc
    11221 gcaccaggac ggtgttgccg aggaggagca gtatgatctc cgagacggcg accgatccaa
    11281 acgagatcag gtacggcagt ccggcggcga agtggagcac cgtggcgacg ccggcggcga
    11341 tgatcagggc gaagacgagg ctggcgaggt aggtcgcgaa tacgcctccg attcgccggg
    11401 ccagcaccca gcacagggct ccgccgacga ggttcactat gggcatcaag atcaactcca
    11461 gaccgccggc ctgcggggag aagaggttca cgatcccgac cccgaccgcg aaggccggta
    11521 tcgtcgccgg gtactttatg accagcggct tcaacacctc gccgagacgg aactggatcg
    11581 ggccgtaggc cagcggcgct accagcagac tcaacaccac gtacagcgcc ccgaccaccc
    11641 cggcgatcgc gacccgtctt gcgggactac ctttcagcat atctataact cctcgcctac
    11701 tgcgctaagg cgcagccgtc cttgcgcggg tcgctgccgc aggcccgcgc gccgctctcc
    11761 atgacccgga tgatctgcgc cccgccgacc ggcacgatgt agtcctcccc gacctccacc
    11821 tcgtgaccta gggagcgcag ttcctcgacc tccgcctccg gcacccggcc ctcgacgagc
    11881 aggacgtttg agttatggtc gtggaagtgc cggggatgat ccacggtctc ctgggggttc
    11941 atgccgtgat ctatcatgtt ggagagcacc tggaagtgcc cctgcggttg catcgggccg
    12001 cccatgttcc cgaagacgct ccagagcgcg ccgtcgcagg tggcgagtcc ggggatgatg
    12061 gtgtgcaacg ggcgctttcc ggggacgagg acgtttgggt gatccgggtc caggcggaag
    12121 gagtggccgc ggttctggag gcatactccg gtgcccccgg ccacgacgcc gctgccgaag
    12181 cccatgtaga ggctgttgat gaacgagcag ccgtttccct cggcgtccac ggcgcagagg
    12241 tacgtcgtat cgtcgcccaa ggccgggccc acggatactc cgacgcggtc gcgcgatatg
    12301 cgcccgcgca gcttcgcggc gtagtccttg gagagcaact cttccgtcgg cacgcccgcg
    12361 aactccgggt ctcctacctt cgcgtgcagg tccgcgaaag cgagcttctt cgcctcgacc
    12421 tccaggtgga tgcgctcggc gccgagcggg tccaaggccg acatatcgaa accttcgaga
    12481 atgttcagca tctccagcgc cgctatgccc tgtccgggag gcggtatctc gtagacccgc
    12541 acgccccggt agtcggtgtg gatcggctcg acccacgtcg tctcgtgcgc ggccaggtcc
    12601 tccccggcga ggtagccgcc cgcttcctgc gcggcgtccg taatgctccg ggcgatccgg
    12661 cccttgtaga aggcgtccgc cccctcttcg gcgacggcgg cgagcgtctc ggcaaggtcc
    12721 ggctggcgga agaggtctcc cggtctcgga gcccggccat ccggcaggaa ggtactcgcg
    12781 gcggcctcgt tcctcctcag caaggcttcc gccacctccc agtagcgggc gacgacctcg
    12841 gcgacgggac gtccctcctc ggcgaagtcg cgggcgggct ccagaaggtc cgcgagcggc
    12901 aggcgtccga gccggtccgc tgcgtcctcc cagagccgga cggcgccggg gactgtaatg
    12961 gagagtccgc cgcgctcggg gatctcggcg atgccccggt cgcgcaactt ctctatggtg
    13021 gctccgcgcg gggcgcggcc ggagccgttg gcgccgagga cgcggccggt cccggcctcg
    13081 tagaagagaa ggaaagcgtc gccgcccata ccggtcgtca tcggggcgag gacggccatc
    13141 gcggcggcca tcgcgacgca ggcgtcggca gcggtgcctc cggcgtcgag ggcgcggatg
    13201 cccgcgaggg cggcggaagg atgttcgcag gcgacggcgc ccttcgtgcc gaagaccgtg
    13261 gagcggccgc cctgatccgg acgccggacg ctcgtggccg cttcttcgct tcggaacata
    13321 agtctggaac ctctcttaca gtttggtatc aggtgcggag gttagtatag acgagcatgg
    13381 caagctacag agttgcagaa ctacagagga ggtattcgtg ggtaaaggtg cttacgtcgg
    13441 agggacgctg gtctctatct tgatctcgct ggcgctctac ttttccggca ggcgtgaggc
    13501 gggaattttc gtcgggctat gggccccgac catcctgaat ctgggtcaga cacttataga
    13561 cgacgagagc tagaacctcc aagagtcaga aggtctaccg ggcgtccgcg agaggttctt
    13621 atctctcgca cggcgtcttt ccgttgtctg gcgatagtgc cacgtatacg tgctctaacc
    13681 atcctattcg tcctccgggc cggtcgtggc gcgggtcaga cgctcgactt cgtccactat
    13741 gtagaggatc tcttccagcg aagggttgac gacggtcgag ctctgctctc ctatgcggac
    13801 cctcgacggc agtccgggct ccatcgagag ttccagccag gcgagcccct cgccggggtc
    13861 ggcgcggttg cggatggcga ggtccggtcg aagtcgggcg gacgcgctgg tctcgtcttc
    13921 cgggcggtcc gtcgagacct cggcgtgcgg gcgcagcatc cgtagcgcgg acgccagcgc
    13981 ttcctgatag gcccggtact cgtcctcgaa ggctaccaag atgcgcgtct ctccggcctc
    14041 ctctttgtcg tcgcgtcatg gtagcaactc tcgcgtcccg gcacttccgg cgcttcacgc
    14101 cgagcgaggg atagccggag agtccgcgag acggcggacg gcgtcgagta tattctctat
    14161 cgagtcggac ttcgagatca cctccgaggc gcccgcaccg agggccaggt cgtgccgttc
    14221 ggtctccagg ctcacggtca gcaccagcac agcgatatgc ggagcgtact ctctgagcca
    14281 gtggatcagg tcgatcccgt cgccgtccgg gagcgtgaga tctacaatag caacgtccac
    14341 atcttcgaga ccgtcgtcca ccagttcgcc atggcactcg tcgagcgtgc cggcttgcgc
    14401 gaccacgtga aggtcgggct ctccgtcaag aaacagggcg agcgcctgac ggaaccagag
    14461 atggtcctcg atcagcagca gacgccgcgt ctttgtcttc gtcatcaagg cattcctcct
    14521 tgccgagtag ttctcttccg ccagcgcgga agacatgggg ggacgcgggc agaggcccgc
    14581 ggcaaggagg caggtcagcc gggccgctcg gcgacctggg ggataatgga gttcggcttc
    14641 aggacatcct ggaagaaccg ggtggagcct ctggggacgc cgagagccag cataccggcg
    14701 agcacgaaga gcatcaggcc ggtggagccg ttatgacact gccacgaccc gctcgaagag
    14761 ctacaggaag tgccgccgcc agcagggacc gggaaggggg ccggcatcgg aacggaagcc
    14821 ggaccggggg ccggagacgc tggtgcggcg ggagcaggcg cgttcctgga aggttgagcg
    14881 ggagcggagc gctcatgttt gcccgcgaga ctgccgatca ggccggcagc cgcccggagc
    14941 aggctggtag tcatctcgtc agcctgccgg tcgagacgag ccgccttact agaataagat
    15001 ccgaacagga cattcgccgc ctcgattacc cggtccgcga gactccgtgt catgatcgcg
    15061 gctccgcgtc cgtcctcggc gaacgtctga tgtgtttcga acccaccatc gggcaggtcc
    15121 gccgcgtccg taatcggtgt cgccaaaggt agttccgcgg ccaaaacgcc accgctaccc
    15181 gaaggtggct cccgcggaga tacgggcgac gaagcgccca ttggagccgg cgcggaggcc
    15241 agctgagatt gagcagccac cggagcagaa gccacaggcg cgggtgcaag gtccgcaaca
    15301 ggcgcggtcg aatcgaccgg cgctcccgag accggccccg acacctccgg cgacgcagca
    15361 gtttccgata gcgatgggac aatgggagcg ggcgccggtt ccggactcgg ggctggcgct
    15421 acatcgctcg tcggcaacgg cgcggaatct gtagtcggct ccggcgcggg gtcggacact
    15481 ggcgtagggt ccgagaccag taccgggtcc ggtggcggcg aagtatctag cacgggcgcc
    15541 ggtgctgggt cgggagccgg cgtcggcgtc gggtcgggcg ctggtgccgg gtcgggcgct
    15601 ggtgccggac tcggtgaggc ttggtcggtc ggcgcggggt ccgtggtggg cggcgtagag
    15661 gtatccgcgt ctggcgcagg cgtaacgtcg gtcgatgggg cgggctccat tgtcgtcgta
    15721 gaggggtcta gcgaagcgtc actggtggtg tcactgacag ttgtatcggt ggtgggatcg
    15781 gcgctcgcca acgggtcgtc tagcgtctgc gcccgcgcct gtccggttgt cagcagtatc
    15841 gcggcgcaga gcgtggcgag tatcagcagg atctcgacgg tccttccgtg cggctcgcgg
    15901 cggcgctgta ggtggcaaag tctgcttgcc aaaaggttct cctgattccg gaacaggaat
    15961 aaggatacac caccgcacaa agttcatcta tagcaaagcc atctgaaaac acgatgaccc
    16021 ggctaccgcg agacccggaa gttctccccg cctcaggccc tcccgaaatc ccgcagcagg
    16081 tccgcgagcg cgatccgtct gactatcgga cgtccatgcg ggcagacctc cccgaactcg
    16141 cagtcggacc aatcggccag gagcgcccgc atctcctcta ccgacaactc ttctccgaac
    16201 ttcgtggacc ccttgcacgc cagcgccttc gccaggtcct ccccgcccgc cagcgcctcg
    16261 acggccgcca ggagcgccac ctccggatcc gtcaccaggt ccggcgcggc gagcacgcgt
    16321 accgcatccg ctccgaacgg ctcgaaccgg aagccgatgg agttcaactc cgcctccaga
    16381 ccccatatct ccggcgcgag gtcctccgga agccgcgcta ccacggggtc cggcagcttc
    16441 atcgcctcgc ctttcgggtt ctccgagagc cgttcgtaca ggatgcgttc atgcgctccg
    16501 tgctggtcca ggatgatcaa ctcctcaccc tcctgagcga ctatgcacct gcccgcgaac
    16561 tgcccgatga gctttagctc cgtgttccgt tcgtccggcg ccgcgccacc aacgacctcg
    16621 cgctccaccg ccagcgccgc ccggatggtc ccgaccacca gcggataggc cgagcgcccg
    16681 ccccgcagcc gcaccgcgct cttcgccggg tggacgttca catccacctc gcccggtccg
    16741 agatccagcc gcaggaacgc cgccggatgc cgtcccttcg gcaagagatc cgcgtaggct
    16801 ttcgccaccg ccggcgcgaa cgagtccgta tccacgaccc gcccgttcac cgcgacgtgc
    16861 aggtagcgcc gggtagggaa gctcaccgag agtgggctca cgaaccccca caccaccggg
    16921 tcctccagtt ctaccaggct ccgcgccagc gagagaccgt gagcctgaga gatacgctcc
    16981 cgcaggtctg ccgccgcggg cagcgccagc acgcctcgcc cgtccgagcg caggaagaag
    17041 cctgcctccg ggcggctcag ggcgagagct tgcaccacgg tcgccaccgc gttcgcctcg
    17101 gcccgcgccg tgcccaggaa gcccctgcgc accggcaaat tcaggaacag gtcccgcgcc
    17161 tcgaccgtag tgcccgacgg atgggcggcg ggtccggcct ccaccgtctc tccggccagg
    17221 accctcaccc gcgtgccgcg ggcggcgccg cgttcgcacg tcgtcagagc gagcaaagag
    17281 accgaaccga tagcgtgcag agcctcgccc cggaagccca gagacacgac gcgggccagg
    17341 tcgtcggccg tctcgatctt gctggtcgcg tgctccgcca ccgcgcgctc cgcgtcctcc
    17401 ggcgacatcc ccgagccatc gtcccgcacc cggatcagag acaccccgcc accctcgatg
    17461 tccacctcga ccctcgaagc tccggcgtcc agcgagttct cgaccagttc cttcaccgcc
    17521 gatgccggtc gttctataac ctccccggcc gcgatcctgt gcgccacctc cggcggcagg
    17581 acatggacct ttcccccgta ctccgccaac tcttcggcaa tgagacgcgg aaagagtttc
    17641 accccgcgaa gtccttgacg ccctcggctt cgaggcgggt gaggatctca ccggcccggt
    17701 ccgttacctg gcgcggcagt ccggcgagcc tcgcgacgtg aactccgtag gacgacgact
    17761 ccgcgccggc ttctatcctg tgcaggaaca cgatctcgcc tcccaactcc tcgacgctcg
    17821 ccttgtagtt gcggcatccg ggcagtatct ccgcgagccg cgtaagctcg tggtagtgtg
    17881 tggcgaagag cgtgagcgac tgcacgtcgt cgtgcaggta ctcggcgatg gcccaagcga
    17941 ggctcatccc gtcgtaggtc gaggtcccac gcccgacctc gtccaggatg accaggctcc
    18001 gctccgtcgc gccgttcagg atgctcgccg cctccgtcat ctcgaccatg aacgtggact
    18061 ccccgctagc cagccggtcc tccgccccga ccctggtgaa gatgcggtcc actacaccca
    18121 gggtagcctc atccgccggg acgtaggagc cggtctgggc caggagcgta atgatggcga
    18181 cctggcgcag gtagacggat ttgccggcca tgttggggcc ggtgatgatc tgtaaccgcg
    18241 agtccccgta cacttccgag tcgttgggca cgaagggtgt ccccgtgttg tgctccacga
    18301 cggggtggcg ccccccgatg acccggatgc cgcgcctctg ggtcaccgcc gggcggcagt
    18361 agcgaagctc ggtcgcggcg gcggcgaacg aggctaccac gtccacgacg gccacggctc
    18421 gggctatctt ctgcagctcc ggcgcggcct ccttcacggc ggcgcggatc tccgagagga
    18481 tccggcgctc cagccgctcc acctcgtcgc gcgccgtgag catccgcgat tcgtgctcct
    18541 tcaactcgac cgtgacgaag cgggcgttgt gctttagcgc cttgcggtgc tcgtagcgat
    18601 ccggcaccgc gccggtgtcc ttgcccgcca cctcgatgaa gtagccctcg ccatcccggt
    18661 agccgacctt caggcttttc agtccggtct tgaggcgctc gtcggcctcg aagcgggtca
    18721 gccaatcgtg cgccccgtcc cggaactcgc gggcctcgtc cagctcgctc gaatagccgg
    18781 cgcggatgac ctcgccgctc tcctccgaga tcgccgccga gataagctcc cgcacgcccg
    18841 gcggctcctc catcgccgcc agcactcgcg agagcaacga gcttctttcg gtcgccggag
    18901 ccaacacttc cttgagcggt ccgatggcct caagggctcc tttgagcgag agcaggtcgt
    18961 tcggggaggc ggagaggcgg actatccggg tggcgatgcg ctcgatgtcc gggatgcgag
    19021 agagcagctc gcgaacctcc tcgcgcagca tgtagtccgg cacgagggcg tccaccgccg
    19081 cgagacgctg ctcgatgcgg tccacctcca gcagcggccg ctccagccag cgcctgaggg
    19141 cgcgctggcc catcggggtc ctggtgcggt ctatcgtctc gatcaggtcg tccaaaccca
    19201 ggttccggcg ggtggcggcg tcgaggacca tcccggagcc ggggtcgtaa ggccggaagg
    19261 tcacgacctg ctccggcgcg tgcccgccac gcaatgacgc aagatattgc aacaatgcgc
    19321 cagccgcccc cacgagcgcg ggccgcccat caagtccgaa ccccttcaac cccgacacct
    19381 ggaagtgccg cttcagagcc tgctctccgg ccgacggctc gaacgtccag cgcggcgcgc
    19441 tgctcaccgt ggcgcgcacc ttcggcaggt tctcggcggc ggtccgctcc ggcacgacca
    19501 gctcccgagg ctgccagcgc tccagctcgg cggccaactg ctcctccggc acctccgtgc
    19561 cggtgaactc gccggtggac gcctcgacca ccgctatccc cgcctgcccg tcccggacca
    19621 cgaacgccgc caggtagttc gcctctccgg cggagaggac gttgtcctcg atgacggtcc
    19681 ccggagtaag tatccgggtc acttcccgcg tgaactgctt cggcttcgtc gggtgcgggc
    19741 gctgctcggc cacggcgacc gagtagccct tgcgcagcag cgtcgcaagg tgttcctgca
    19801 gggcgtgtcc gggcactcca gcgagcggga cgcggcctcc gtcgtagccg gcggcctcac
    19861 ggctcgtcag gcggatggag agggcctgcg aaatcttctt cgcgtcctcc tcgaaggtct
    19921 cgaagaaagt accaacctgg tagaagagga tcgtccccgg cggcagttgc gctttcagct
    19981 ctgcgtggcg tcccagcatg aggctattct acggcccgat cccccacccg tcagccccca
    20041 acctcgcaca ccgaactacg gtgtatcctg agcccgtgta cgacgcggac cccggactat
    20101 cgaaagttat cctcgcccat gccgcactcg gcgccgcgct gaacctggct ttctcgggca
    20161 gggaccgcgg tctgcgccgc gctctcgtat tgttcgcgct cggcgccggc ctgccggcgc
    20221 tgggcgagct tctcgtcacg gctcctctgg gtctcctgcg tcaccgcacg cggccccgag
    20281 tagcgggtgt gccgctcgga gtcctgctcg gctggtactg cgctatccac ggctccttca
    20341 cggtgacggc gcggattctg gcgcgtttac cgatagatga ggaccggaga cacgcggccc
    20401 tgccgttcgg cgcggcgctc gtcgggacga gcctcgacct gatcctggac ccgttcggcc
    20461 tggacgccgg tctctgggag tggactaccg acggcgtcta tgcgccggag gtagtgggcg
    20521 ccaacggacg cagcggcgta ccgcttatca actacctcgg ttggatctcg ctggtggcga
    20581 gcgtcgtgta cgtccacaaa cgtctcttcg aggacgggac ttcgccggac ctggacgcga
    20641 gccgcatccc cggtttgctt ctcttaccgt actacctggc cgcggccgtc tgggcggtga
    20701 ggcggcgaag gcttcggtac ctggtctact cggtcatctt tccggtcgcg ctgggcgtga
    20761 gcctgagagg ccggtaagcg aagttcgcta gtcttgagag tgcgggtaga tgaggaccgg
    20821 cccgtcggcg gcgcggacta ccttcgtgga gacgctcccg acccgcgacc gttggatgcg
    20881 ccccaggccg cgggagccga ccgagatcat ggtcggctct cctgtctccg acgctgtgtc
    20941 caggagcgtc agcgctatgc cgtcgatgcc gtccgcgtcc tcatccacca tcagcttcac
    21001 gtcgggacgg ctccccagga tacgctccag gtcttccgct ctctcccgta gctcggcctc
    21061 cgcctgcttg agggcctgct cgaccatccg gacctcaaga gggctgtgcg tctgggaaga
    21121 cttcaagaga cgtggatata cctgaaccag agagcctcgc gccccgaaga gcgccccgat
    21181 gctggccgcc aactccgccg acttccccgc gtcatccgag gagtcgtccg ccattattat
    21241 gcgcgagggc ggccaggcgt cgtcctcgca gcggagaacc agtaccgggc agcgcgtgtg
    21301 gtggacgacg ccctccgaga cgctgcccat caggaggcgc ttcacgccgc ccagacccct
    21361 gcgacccacg atcaagagcc cggccccgat ctcctcgctg gcggtcagga tctgatctac
    21421 cggagagccc atcatgagcc gggcctcagc gaccacgccc ccggactcct cgatgcgctc
    21481 cacctgctcg tccagcagcc gctgtccgtg gcacgtgaat tccggagagc gcagcctgag
    21541 cgcgacgctc atgtactccc gcggcgggat gaactcgaag gcgtgtacga cgtgcagctc
    21601 cgagccgctc cgccccgcca ggtcggtcgc cgctcgcctc gcaacggcgg cgtcctccga
    21661 cccgtcggtc gccagcagta tcttcgtcgg gaagtatccc atctcctgct cctttccccg
    21721 tcgctataag acggccacca ccgccccgac cacgaacgcc gcgtagaaaa cgcccccgat
    21781 caacaagtgt cccgcccgta gcttcccgga gctcttgagc accaggtacg cgatccccgc
    21841 cgagacgagc gccagcgcca cagcgaagag gtccagcccc gtcaggtccc actccgtgaa
    21901 caggatgccg aacgtcaccg ggatggtgct ctggaagatc atggccccgg tgacgttccc
    21961 catagccagc gtatccttgc cgtccctgac ccagaagatc gagttgaact tctccggcag
    22021 ttcggtggcg agcggcgcga gcaccagcgc gatgaggccc gccggcagcc ccaggcccgc
    22081 cgccgcgtgc tcgaccgcgt ccacgaagaa gtgcgctccc gcgacgatgg ctcccaggct
    22141 cgccagcgcc tgcgcggcta tggcgaacgt cggcgggttt tgggtccggg gcaggaacag
    22201 gagcctctcc ggcacgttct ccaggtgtcc gccggaggag agggtcagct tcgcgtacca
    22261 ggcgtatccg gccacaagtg ccagcgccac gacgaccttc aggacgagcg gcaggtccac
    22321 cataccaacc ccggcggcga tggcgaagaa gaccaggaag aagccgatat cgcggccgat
    22381 ggtcgcgttg tccacgctca ccgccgagcc ctgcgagcgg cgccgccgga agaccaggac
    22441 cgaggccccg acgacgaaca gggcgagcgt cgccagcatg aacggggcgc ccaggatcgc
    22501 gccgatcccg atcgaggcgc cgtcgccgga agtgtcgccg ctaatgacgg gctgcaggat
    22561 ggcgaccacc gcgatcatgc tctccggcaa cgcggtgccg acggcggcga gcaggctgcc
    22621 taccgctccc tgttgcatcc ccagccgccc gcccagaagc tcgaccgcgt tcgtaaaaaa
    22681 cgctgcgccg agcaggatga ccactagtgc cagtacgaac tctattatcg tgtacaaggg
    22741 gttcctttct gatttctttt ctgataggta ctcgtcattg ccgggtctct tccggcgggg
    22801 tcctgccgtt gcgggtcttc cacagcgagg ccgctatcga ggccgccacc acgacggcga
    22861 tgaaaggcag cgacacccag atcgggacct tcgcgtcgaa gatgccggag tagaaaaact
    22921 tcgcccccac gaagatgagg attatcgaga ggccgaggct caggtagacg aaacggtcga
    22981 tcatcccggc gagcatgaag tacagcggcc tcaagcccag cacggcgaaa gcgttggccg
    23041 accacaccac gaaggcgctc gaagttatgg agaagatggc ggggatggag tcaaccgcga
    23101 agaccacgtc cgtcgtcccg atcatcacga t
//
LOCUS       DASSMB010000243         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1704818, whole genome shotgun sequence.
ACCESSION   DASSMB010000243 DASSMB010000000
VERSION     DASSMB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1704818"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(603..1400)
                     /locus_tag="VFJ72_14025"
     CDS             complement(603..1400)
                     /locus_tag="VFJ72_14025"
                     /inference="COORDINATES: protein motif:HMM:NF021465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HET7480620.1"
                     /translation="MQQASSFSPAVLQRWRRRENLLPGLTLLALAAAGWAYTVRRATE
                     MDNMGGAMPPVATGDIGGFAAFVFGWTAMMVAMMVPATLPMILLYRTISRKRLGLAPA
                     RAGVSFLLAGYIGVWAAVGLPVYVGFLLGEPLGSAGALLPGLLLVAGGVYQFTGLKKS
                     CHARCANPLFFLMRQWRPGAGGALRLGTLHGADCLGCCAGVMLALVALGMMNLAWMLT
                     AAVVIFIEKTIGSHRIARPLGVAMVAAGLALLGASWLDTTPPGMGLM"
     gene            complement(1449..2060)
                     /locus_tag="VFJ72_14030"
     CDS             complement(1449..2060)
                     /locus_tag="VFJ72_14030"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HET7480621.1"
                     /translation="MAWRLEGTYFENCSCDMVCPCGTSALTMPADYDRCQVVLAFHVD
                     SGEVDSVDVSGLTVAMLADTPPVMGEGNWRVGLFMDESASQEQSDKLGAVFSGQMGGP
                     PELVASLIGENLGMEVAPIEYVDDGLRHWVKIGDFVEMEIEDFVPPQTPGAEPTKITG
                     VFHPANSTLTVARATNNSQVSAFGLEFSNAEKNGHSAPFSWSG"
BASE COUNT          446 a          805 c          738 g          404 t
ORIGIN      
        1 ccgtccagcg ctgggcgtag gggtagcggt gggtggggac ggcgttgtcc atggggatcc
       61 agccccacgt cagggagtcc ccgtagcaaa ggatgcgctt cttctcggcc gtgccctgtt
      121 gctccatgcc tctcctcctg gttctgttac ggttccgtga ccgtgcggaa cggaagtata
      181 gggtacgctc catcgcgccg cccggcgctc tcgtcccgag tgtccgcgtg gacggcggtg
      241 gcatgggctt acgagggatc ggggcgtccc ggcccccggg cctccaaaga ggacatgggc
      301 tgtcggcacg cgccccggat ttgcgaagct ctcccccctg agccggagtt ttcccgacaa
      361 tgttggtctc gctacctccg tagccattgt gacggggtta ctgcggtcaa ggtcatctgc
      421 tacgaggacc atctactacg aagaagacgg catagccatg atctacctcc gcgagcgtat
      481 acggtggaca aggtcgtggg tgaggacact ctcaagggct tgcccgctct cgacctggag
      541 ggaccgagcg cactttagaa gaactgtagc ggcataccgg attacaccgg cttgcggtcg
      601 tcttacatga gacccattcc gggaggcgta gtgtcgagcc aggacgcccc cagcagggcc
      661 agcccggcgg cgaccatcgc aacacccaga ggccgagcga tgcggtggct tccgatcgtc
      721 ttctctataa agatcacgac ggccgccgta agcatccagg cgaggttcat catgcccagc
      781 gccacgagcg cgagcattac acccgcgcaa cagcccaggc agtcggcccc gtgcaacgtc
      841 cccagccgca gcgcgccgcc ggcgcctgga cgccactgcc gcatgaggaa gaacagcggg
      901 ttggcgcacc gggcgtggca acttttcttt agccccgtga actggtagac gccgccggca
      961 acgaggagca gccccggcaa caacgctccg gcggagccca ggggctcgcc gagcaggaag
     1021 ccgacataga ctggtagtcc caccgcggcc cagaccccga tgtatccagc cagcaggaac
     1081 gacacgccag cccgcgccgg cgcgaggccg aggcgtttgc gggagattgt gcggtagagc
     1141 aggatcatgg gcagcgtggc gggcaccatc atagctacca tcatcgccgt ccagccgaag
     1201 acgaacgcgg cgaaccctcc gatgtcgcct gtcgccaccg gcggcatcgc gccgcccata
     1261 ttatccatct cggtcgctcg acggaccgta taggcccacc ccgcagcggc cagcgccagg
     1321 agggtaaggc ccggcaagag gttttcgcgc ctgcgccacc gctgaagcac agcgggcgag
     1381 aacgaactag cttgctgcaa actccctcct ccgccgaagg tctacctcgg agacgacgcc
     1441 cggcgtggtt atcccgacca ggagaaaggc gcggagtgcc cgttcttctc cgcgttggag
     1501 aactccagcc cgaacgcgct gacctgggag ttgttcgtcg cccgagcgac ggtcagcgtc
     1561 gagttggccg ggtggaagac acccgttatc ttcgtcggct cggctcccgg cgtctgcggc
     1621 gggacgaagt cctcgatctc catctccacg aagtccccga tcttaaccca gtgccgcagg
     1681 ccgtcgtcca cgtactcgat gggagcgacc tccatgccca ggttctcccc gatgagactc
     1741 gcgaccaact cgggggggcc acccatctgt cccgagaaga cggcgcccag tttgtccgac
     1801 tgctcctggg aggcggactc gtccatgaac aggccgaccc tccagttccc ctcgcccatg
     1861 accggcggcg tgtcagcgag catggcgacc gtcaggccgc tcacgtcgac gctgtcaacc
     1921 tcacccgagt cgacgtgaaa tgccagcact acctggcagc gatcatagtc ggcgggcatc
     1981 gtcaacgccg aagtaccgca cggacacacc atgtcgcacg aacagttctc gaagtaggtg
     2041 ccttccaatc gccacgccat tattacctcc gtccctcgcg cttccccacg aagcattgtg
     2101 attataataa cattacgcct ctggagttgc caggacatgt ggcggcacgg caggcttgtc
     2161 ctcgatctgg tctggcggct cctagaaaag atacgatgct gcgttcggag gccatctccg
     2221 agaagccgac cgggtccgtg aacttcgctc caagagaggt caggtcttct tccgtcaccc
     2281 cacgggcgac cgcgcaagac cctcagacgt agactggtat ctcgaagtcc ctgaccttct
     2341 ggacgagctc gcggagcggc gggaagccga caccgtggac ggcgtctacc atg
//
LOCUS       DASSMB010000244         6190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_585661, whole genome shotgun sequence.
ACCESSION   DASSMB010000244 DASSMB010000000
VERSION     DASSMB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585661"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFJ72_14035"
     CDS             <1..463
                     /locus_tag="VFJ72_14035"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7480622.1"
                     /translation="TVTVAVLENNEAVVIHQAQSNSSALSVDWTGRHTPIHATGAGKV
                     FLAHMPGNQLDSIMADPLERFTEHTVVEPARLREDLEDIRARGYAICFEELETGLNVI
                     AAPVRFARGAITLAISVSGPAFRFPPDGAGDIGELTRGAADEISRCLGFWE"
     gene            706..1359
                     /locus_tag="VFJ72_14040"
     CDS             706..1359
                     /locus_tag="VFJ72_14040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480623.1"
                     /translation="MNSLSFPDYLASRWDDLLELSIEHVQVVLISLLIATVIGVSLGI
                     LTYRRPAAAQVVLAVASTILTIPSFALFGLLIPLLGLGYKPTVVALSAYAVLSILRNT
                     ITGLRSVDPAITESAKGMGLSNWQQLVKIELPLAWPVIIAGIRVSALLILGIAAIAAY
                     VNGPGLGGDIFSGLSRIGGANALNLALGGFLGIIVLALLFEVLFSVIGRLTTSKGLY"
     gene            1359..2540
                     /locus_tag="VFJ72_14045"
     CDS             1359..2540
                     /locus_tag="VFJ72_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7480624.1"
                     /translation="MDGAKDVDSTHAGTMIRLEHLSKTFPGQHEPAVDDLSLEIYEGE
                     IVVFVGPSGCGKTTTMKMINRIIEPSSGKIYIEGEDVTQADPDKLRRRIGYVIQQIGL
                     FPHMTIADNIATVPKMLGWDKKRISDRIDELLETVHISKTYRDRYPKQLSGGQRQRIG
                     VARAMAADPPIMLMDEPFGAVDPITRERLQDEFLRLQQDIKKTIVFVTHDIDEAIKMG
                     DRIAILRDQSKIAQYDTPERILTDPADEYVEDFIGSGASLKRLSLSKVRDIETSDWPV
                     AGLDAGHGEVREMMRRSGKEYVLLLDEQRKPKRWVSLSDVEHDGVPLSEAGWPAVAIV
                     DGGSSLYDTLDTMITSYKGSAIVVDGDGRYQGVVDFETVLNAIEDMRPHDDRRPLRER
                     A"
     gene            2537..3373
                     /locus_tag="VFJ72_14050"
     CDS             2537..3373
                     /locus_tag="VFJ72_14050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480625.1"
                     /translation="MRSTEEDRPSTISEDLYSSSAPEVSSGPPVEGKGPGTARTLARY
                     LVMPVILAAVCAGLYLWVGSLNLDSIEQRSLNREVITEKLTRHLEITAVATVIVVVLA
                     VATGVLLTRPAMRKVAPYIAAVASTGQGIPAIGVLVLSAILLDQFGFKIAVAALVIYA
                     FLPVLRNTMVGIRQVDSAVIESGRGMGMTKTAVLFRIELPLAIPIMLAGIRTALIIVV
                     GTATLATFINAGGMGDIINTGIKTSREPILITGSLLTAVLALGVDYIAGIAEDVLKPR
                     GL"
     gene            3443..4402
                     /locus_tag="VFJ72_14055"
     CDS             3443..4402
                     /locus_tag="VFJ72_14055"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7480626.1"
                     /translation="MSRSVLRMVGLLVVMVMVAAGCGGAGGGGGGGSASDVDLSDASF
                     TVGSKDFTEQLILGQITLQALEAAGANVDDQIGLSGTDATRQALLSKDIDIYWEYTGT
                     IWINHLKHTKPIPDSKKQYEAAAKEDLQKNKLKLLERAPFNNTYALAIRQEAYNKVGV
                     KKLSDLKNLVKDNPDEATVCVESEFNSRDDGLPGMEKAYGFQFPKDNIALFDTGVVYQ
                     RTDKGDPCNFGEVFTTDGRISALKLKLIQDDKNFFPIYNPAPEVREETYKEYGKQLDK
                     IFTPIAKKLDTKTMTQMNAEVDVKGQLPEDVAKDWLEKNGFVD"
     gene            4490..5002
                     /gene="fae"
                     /locus_tag="VFJ72_14060"
     CDS             4490..5002
                     /gene="fae"
                     /locus_tag="VFJ72_14060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HET7480627.1"
                     /translation="MSFAGHALIGESFVGDGANAAHINVVIGRRGGPVETAWTTALAT
                     PRMGHTPFVAVLQPGLPVLPLTLFVNKAAMESELHGNLTWGAAQAGVASGVAAAVADG
                     LIPQDEVHDLLLIAAVWVNPEAESEEEVFANNRESARGAIEAAVNRLPAVEDVLAERD
                     DPRNPFYGSS"
     gene            5006..6121
                     /locus_tag="VFJ72_14065"
     CDS             5006..6121
                     /locus_tag="VFJ72_14065"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET7480628.1"
                     /translation="MKITGIRILRLARELDPPFHAAWDPTPRRTFEATLVFVETSEGI
                     TGVGSGDTMAGFEGYEHLFVGEDPLAMVRHVRAIESINFHAGRYWPLEAALWDIAGQV
                     CGQPVATLFGGAVKRVPAYASCGELKPPEERAEVALALREEGFRAMKIRVDPERLEEG
                     IASVRAAREAVGSSMEIMVDLNQAWRMAGDARRSIDPVTAREIAGRLRDLDVFWLEEP
                     LDASDAKGLADLRAASGVRIAGGEMARTVPELLAYIEADSLDVYQPDVVLAVGMMRAR
                     TVAELAMARNRHFTPHTWTNGIGVLANLHVTAGVSGGPYLEFPYDPPGWTPERRDFML
                     AEPLRIDAGGCLNVPDLPGLGVVLDEDVLRGLGKVTG"
BASE COUNT         1163 a         1853 c         2128 g         1046 t
ORIGIN      
        1 gacggttacg gtcgcggtcc tggagaacaa cgaggccgtc gtgatccacc aggcgcagtc
       61 gaactcttcc gcgctgagcg ttgactggac ggggaggcac acgccaatac acgctacggg
      121 cgcgggaaag gtgttcctcg cgcacatgcc ggggaaccag ctagatagca tcatggcaga
      181 ccctctggag cgcttcacgg agcatactgt ggtagagcct gcgcggctgc gggaggattt
      241 ggaggacatc cgggccagag gctacgctat ctgtttcgag gaactggaga ccggcctgaa
      301 cgtgatcgcg gctccggtgc ggttcgcgag aggggccatc acccttgcga tatcggtctc
      361 cggtccggcc ttccggttcc cgccggacgg cgccggagac atcggcgagt tgacccgcgg
      421 ggccgccgac gagatatccc ggtgtctggg gttctgggaa tgatgggctc gccggggcgc
      481 cggatgcctg tgagcgtcaa agagacgcgt gaattgacag ttggccacac attcgtcaga
      541 attcactgtg cgctggggat ggtatcggcg ggatgagggg gcaggcaata aggcgcgcag
      601 gcctcgtctt gttcgctacg cttcgcgacc atgaagagac gatgtacagc accgagcgct
      661 agaggggatt gcatttatgc gccgcactga gcatcaggta acggtatgaa cagtctcagc
      721 tttccggact acctggcgtc gcgctgggat gacctcctgg agctttcgat agagcacgtt
      781 caggtggtgt tgatctcgtt gttgatcgcg acggtcatag gggtctcgct tggaatcctt
      841 acctaccggc gtccggcggc ggctcaggtg gtgctggctg tcgcctcgac gatcctgact
      901 attccatcgt tcgcgctgtt cgggctcttg atccccttgc tcgggctcgg gtacaagccg
      961 acggtcgtcg ctctctcggc ctacgcggtg ctctccatcc tgcggaacac gatcacgggc
     1021 ctgcgctcgg tggacccggc catcaccgag tccgcgaagg ggatgggact gagtaactgg
     1081 cagcagctcg taaagatcga acttcctctc gcgtggccgg tcataatcgc cggcatccgg
     1141 gtctcggcgc tcttgatcct gggcatcgcg gccatcgcgg cgtacgtaaa tgggccgggg
     1201 ctcggcgggg acatcttctc cggtctcagc cgcatcgggg gggctaacgc gctgaacctg
     1261 gcgctcggag gatttcttgg gatcatcgtg ctggcgctgt tgttcgaggt gctgttctcg
     1321 gtgattggca ggctcactac atcgaagggg ctctattgat ggacggcgct aaggacgtag
     1381 atagtacgca cgcggggacc atgatccgcc tggagcacct ctcgaagacg tttccggggc
     1441 agcacgagcc ggcggtggac gacctctcgt tggagatcta cgagggtgaa atcgtggtct
     1501 tcgtcgggcc ttccgggtgc ggcaagacga cgacgatgaa gatgatcaac cgcatcatcg
     1561 agccgtcgag cggcaagatc tacatcgagg gcgaggacgt tacacaggct gacccggaca
     1621 agctccggcg ccgcatcggg tacgtgatcc agcagatcgg gctcttcccg cacatgacca
     1681 tcgcggataa catcgccacc gtcccgaaga tgctcggctg ggacaagaag cgcatctccg
     1741 accggatcga cgagctgctc gagacggtcc acatcagcaa gacgtaccgc gaccgctacc
     1801 cgaaacagct ctccggcggg cagcgccaga ggatcggggt ggcgcgggcg atggcggcgg
     1861 accccccgat catgctcatg gacgagcctt tcggggcggt cgacccgatc acccgtgaac
     1921 ggctccagga cgagttcctc cggctgcagc aggacatcaa gaagaccatc gtcttcgtca
     1981 cccacgatat agacgaggcg ataaagatgg gcgaccgtat agccatcctg cgcgaccagt
     2041 ccaagatcgc ccagtacgac acgccggagc gaattctcac cgaccccgcg gatgagtacg
     2101 tcgaggactt tatcggctcc ggggcttccc tgaagcgcct cagcctgagc aaggtgcggg
     2161 acatagagac gagcgactgg ccggtggcgg gtctcgacgc cggtcacggc gaggtgcggg
     2221 agatgatgcg gaggtccggc aaggagtacg tgctgctgct ggacgaacaa cggaagccga
     2281 agcggtgggt gagcctgtcg gacgtcgagc acgatggcgt gccgctctcc gaggcgggct
     2341 ggccggcggt cgccatagtg gatggcgggt cgagcctcta cgacacgctg gacaccatga
     2401 tcacgtcgta caagggttcg gctatcgtcg tggacggaga cggccgttac cagggagtgg
     2461 tcgacttcga gacggtgctc aatgcgatag aggacatgcg gcctcacgac gaccgccgtc
     2521 ccctgcgtga gagagcatga gaagcacgga ggaggataga ccttcgacga tctcggagga
     2581 tttgtattcc tccagcgcgc cggaggtcag cagcggtccg cccgtggagg gcaagggtcc
     2641 gggtaccgcg aggacacttg cccgatacct ggtgatgccg gtgatcctcg cggcggtatg
     2701 cgcggggcta tacctctggg tggggagcct taacctcgac agcatagagc agcgctcgct
     2761 caaccgggag gtcatcaccg aaaagctcac gcggcacctg gagatcacgg cggtcgcgac
     2821 cgtgatcgtg gtggtgctgg cggtcgccac aggggtgttg ttaacccgtc ccgcgatgcg
     2881 gaaggtggca ccctatatag cggcggtggc gagcaccggg caggggatac cagccatcgg
     2941 cgttctcgtg ttgagcgcga tcctgctcga ccagttcggg ttcaagatcg cggtggccgc
     3001 gctcgtcatc tacgcttttc tgccggtgtt gcggaacacg atggtgggga tacggcaggt
     3061 ggactccgcc gtcatagagt ctgggcgggg gatggggatg acgaagacgg cggtgctgtt
     3121 ccgcatagag ttgccgctcg ccatacccat catgctcgcg ggtatcagga ccgccctgat
     3181 cattgtcgtg ggaacggcga cgctggcgac tttcataaac gcgggcggga tgggcgacat
     3241 aatcaacacc gggatcaaga cgagccgcga gccaatcctt attacaggct cgcttctgac
     3301 tgccgtgctc gcgctggggg tggactacat cgccgggatc gcggaggacg tcctgaagcc
     3361 ccgtggattg tgagcgcggg gttatccata atcgttagcc gtcgggttgg ccgtcggaac
     3421 ggaaccaaaa aggggaggaa ccttgagtag atctgtgttg cggatggtgg ggcttctggt
     3481 cgtgatggtg atggtcgccg cggggtgcgg cggggccgga ggcggaggcg gcggtggcag
     3541 cgccagcgac gtggacctct cggacgccag cttcaccgta ggctccaagg acttcaccga
     3601 gcagttgatc ctcggccaga tcacgctgca ggccctggag gcggccgggg cgaacgtgga
     3661 tgaccagatc ggtctctccg gcaccgacgc gacccggcag gcgctcctga gcaaggacat
     3721 agatatctac tgggagtaca cgggcaccat ctggatcaac cacctcaagc acaccaagcc
     3781 catccctgac agcaagaagc agtacgaggc ggccgcgaag gaagacctcc agaagaacaa
     3841 gctcaagctg ctggagcgcg ccccgttcaa caacacctac gctctcgcca tccgccagga
     3901 ggcttacaac aaggtcggcg tgaagaagct ctcggacctg aagaacctgg tgaaagacaa
     3961 cccggacgag gcgacggtct gcgtcgagag cgagttcaac tcccgtgacg acggcctgcc
     4021 gggcatggag aaggcgtacg gcttccagtt ccccaaggac aacatcgcgc tcttcgatac
     4081 cggcgtggtc taccagcgca ccgacaaggg cgacccctgc aacttcggcg aggtatttac
     4141 gactgacggg cgcatctcgg ccctcaagct gaagctcatc caggacgaca agaacttctt
     4201 cccgatctac aaccccgctc ccgaggtccg cgaggagacc tacaaggagt acggtaagca
     4261 gctcgacaag atcttcacgc cgatagcgaa gaagctggac accaagacca tgactcagat
     4321 gaatgccgag gtggacgtca agggccagct cccggaggac gtcgccaagg actggctgga
     4381 gaagaacggc ttcgtcgact agaccagcga taacaggctc cccggcgaga acagtaagca
     4441 aggcgtttct cgccggggag tacatccaac aattcaagga gatcgctcga tgagcttcgc
     4501 cgggcacgcc ctgatcggag agtccttcgt cggagacggc gctaacgccg cccacatcaa
     4561 cgtcgtcatc gggcgccggg gcggacccgt tgagaccgcc tggacgaccg ccctggcgac
     4621 accgaggatg ggccacactc cgttcgtggc cgtgctccag cccggtctgc cggtcctgcc
     4681 gctgaccctg ttcgtcaaca aggcggcgat ggagagcgag cttcacggca acctgacgtg
     4741 gggcgcggcg caggccggcg ttgcgagcgg agtcgccgcc gccgtagccg acggcctgat
     4801 accgcaggat gaggtacacg acctgctcct gatcgccgca gtctgggtca acccggaggc
     4861 ggagagcgag gaggaggtct tcgccaacaa ccgggagtcg gcgcgcggag cgattgaggc
     4921 cgccgtgaac cggctcccgg ctgtcgagga cgtgctcgca gaacgcgatg atccgcgtaa
     4981 ccccttctac ggaagctcct agcgggtgaa gatcaccggt atcaggatct tgcgcctcgc
     5041 gcgcgagctt gatcctcctt tccacgccgc ctgggacccg acgccgcgtc gcacctttga
     5101 ggcgaccctc gtcttcgtgg agaccagcga ggggattacg ggcgtcggct ccggggacac
     5161 gatggcgggc ttcgagggtt acgagcacct gttcgtcggg gaggacccgc tggcgatggt
     5221 ccgtcatgtc agagcgatcg agagcatcaa cttccacgcc ggacgctact ggccgcttga
     5281 ggccgcgctg tgggacatcg cgggccaggt ctgcggacag ccggtggcga cgcttttcgg
     5341 gggcgcagtg aagcgcgtcc cggcatacgc ctcatgcggc gagctgaagc cgccggagga
     5401 acgtgcggag gtggctctcg ccctgcggga agagggtttc cgggcgatga agatccgggt
     5461 ggacccggaa cgtctggagg agggtatcgc gagcgtccgg gcggcccgcg aggcggtcgg
     5521 ctcctcgatg gagatcatgg tggacctcaa ccaggcgtgg cgcatggcgg gggacgcgcg
     5581 gaggagcatc gaccctgtga ccgccaggga gatcgccggc cgtctgcgcg atctggacgt
     5641 gttctggctg gaggagccgc tggacgcttc ggacgcgaag gggctcgcgg acctgcgcgc
     5701 cgcgagcggg gtgaggatcg ccggcggcga gatggcccgc accgtgccgg agcttctcgc
     5761 gtacatcgag gcggattccc tcgacgttta ccagccggac gtggtcctgg cggtgggcat
     5821 gatgcgcgcc cgcacggtcg ccgagctggc gatggcccgc aaccgccact tcacgccgca
     5881 cacgtggacg aacggcatcg gcgtgctcgc caacctgcac gtcactgcag gcgtcagcgg
     5941 cggcccgtac ctggagttcc cctacgaccc gcccggctgg acccccgaac gccgggactt
     6001 tatgctcgcc gagccgctcc gcatagacgc cggcggctgc ctgaacgtcc cggacctgcc
     6061 gggcctcggc gtggtcctgg atgaggacgt gctgcgcggt ctcgggaaag ttacgggttg
     6121 acgatctcca caaacgcatt aggctgcgag aaaaccatga tcggagagag ggcatgacgg
     6181 aattgggcaa
//
LOCUS       DASSMB010000245        12895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_585978, whole genome shotgun sequence.
ACCESSION   DASSMB010000245 DASSMB010000000
VERSION     DASSMB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12895
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585978"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VFJ72_14070"
     CDS             <1..443
                     /locus_tag="VFJ72_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7480629.1"
                     /translation="PKKATEIAIQFRPTPHTPFAHDDTKSLDPNVLVVRVQPEEGISL
                     KIGAKVPGQGFEVSSVNMDLLYGTAFLEEAPDAYQRLLLDLMLGDPTLFIRADEAEGA
                     WDILAPVMQAWSEKKEVSMYAAGEWGPEDAEELLRHDGREWRRP"
     gene            428..1513
                     /locus_tag="VFJ72_14075"
     CDS             428..1513
                     /locus_tag="VFJ72_14075"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET7480630.1"
                     /translation="METAMIGESRRAPGGGPMSVEQIEHELAQLRMNEDGTLALRSSV
                     LNLVVVTDEESAGEVTRAVSNLAGRYPSRAIVLISDPEGERNVDVQLSAFCNVRGGGG
                     AQVCAEQITIHAEGPPAEHLDSLAGPLLISDLPVFLWYPGVFSPHSPEFAGMTDISDR
                     VILDSARSGDYEACLRGIHTLLENPETPAVGDLQWVGLSPWRSLLADTFSAPERAGEL
                     EKIERVEVVYAPGGECRALLLAGWLASTLGWSVEGVNRDGDSLQVEFAGPSGDVSFEM
                     SSSSPDARLRRIRIHSAEYSFQVSRHREMSDVRTTVLHGDELVAERTVHIGSFDLGVL
                     VGEELQYRGRDGAYEAALGVAVEILDH"
     gene            1510..2220
                     /gene="pgl"
                     /locus_tag="VFJ72_14080"
     CDS             1510..2220
                     /gene="pgl"
                     /locus_tag="VFJ72_14080"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET7480631.1"
                     /translation="MSVRVYEDKQELAEAAARDFAERAAEAIEDRGRFTVALAGGSTP
                     KATYEILARDYADEVDWSRVHVFFGDERTVPPDHEDSNYRMAREALLDHVPIGSVHRM
                     RGELSPEEAAASYENELREFFGGNLPSLDLVMLGIGEDGHTASLFPNTPALDVTDHWV
                     VENPVEKLDTVRLTLTKPVINAARAVVFAVAGEGKAEALREILEGEVDPHDYPAKFVQ
                     PAGGAVWMVDRSASVRLS"
     gene            2339..2632
                     /locus_tag="VFJ72_14085"
     CDS             2339..2632
                     /locus_tag="VFJ72_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480632.1"
                     /translation="MRKWKVRLSALLAATMMALVFASPAMAQVTIPQVNVPQQVNQAP
                     QVNAGNLVGVNVNALNNVNALNNVSVVVNNVLNNNNVAVNANVLGGTNIVVFQ"
     gene            complement(3069..4112)
                     /locus_tag="VFJ72_14090"
     CDS             complement(3069..4112)
                     /locus_tag="VFJ72_14090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7480633.1"
                     /translation="MDQQRVLITGGAGFLGINLARHLLSRGYAVTSLDVEDFDYPERD
                     RVATIKGDIRDRALLDRAMEGVDFVVHTAAALPLYSPDDIYTTDVEGTRNVLDAALRR
                     GVKRVVHVSSTAVYGIPDHHPLYETDKLEGVGPYGQAKIQAEMICLEYRAKGLVVPII
                     RPKSFVGPERLGVFALLYDWAVTGHNFPMIGSGNNRYQLLDVEDLCDAIALTLALPEE
                     KVNDTFNIGAKEFTTMKEDYQAVLDYAGHGKKVVGFPALPAILGLRVLDRLGVSPLYK
                     WVYETASKDSFVSIEKAERELGYTPKFSNKEALLRNFRWYLDNRKKFENASGVSHRVP
                     WKQGAIGLLKKVF"
     gene            4379..4930
                     /locus_tag="VFJ72_14095"
     CDS             4379..4930
                     /locus_tag="VFJ72_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480634.1"
                     /translation="MLTFAHMEIGGQYDPFRNASRGQSPLALRLGDAPYGPESGRVGV
                     MNSLARSGLPIAEGVVLMEECHRSFLESSGLAWDLLACGDGTEVRKLAQALRRRYRRC
                     TLKEELKGVIRAALAEIGGRSVAVVSEDMTRTGLRSIPRVEEAVLRAWLSPNGLRRQV
                     GAAMRGGEIPLWPVLIQHELHDG"
     gene            5012..5176
                     /locus_tag="VFJ72_14100"
     CDS             5012..5176
                     /locus_tag="VFJ72_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HET7480635.1"
                     /translation="MLAGAVQLGLLGAALADIYRRPADGIRGNKWLWAAASFVNFVGP
                     VSYFVFGRRR"
     gene            5296..5715
                     /locus_tag="VFJ72_14105"
     CDS             5296..5715
                     /locus_tag="VFJ72_14105"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HET7480636.1"
                     /translation="MYHSSPFIRFARWTSRATGHPVSFVAALMIIVAWAVTGPIFGFS
                     DTWQLVINTSTTIVTFLMVFLIQNTQNRDSEAMQLKLDELIRAMNCAHNAMLDLEDLT
                     EEDLNKIKRHYESLARKSREDLKRGLKDTGTIEVRDD"
     gene            5769..6728
                     /locus_tag="VFJ72_14110"
     CDS             5769..6728
                     /locus_tag="VFJ72_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HET7480637.1"
                     /translation="MIVDSAVYVDGRRSAACPLGEIRQTCRESGGFAWVGLHDPSSEE
                     FDSVARELGLSEDMIRDTIREHLRPKVERYGDLTFVVLRAARYLEIPETVEFGEVLAF
                     VGPDFVVTIRRGEAPALSGLRHQLEGEPQTLRRGPMAVLYAVMDRVVSDYGPVVEGLE
                     NDIDEIETEVFGGNSRVSRRIYELSREVIQFHRATQPLAAALKHLSDTEDQYPEFKRR
                     LRVTQDRLLRATEQVEGFRELLTNILSVNLTMVSVRQNEQVQKVSSWAAILVVPTIIT
                     GIYGMNFRYMPELSWSFGYPFSLLLMLAVSGLLYLGFKRSGWL"
     gene            6825..7577
                     /locus_tag="VFJ72_14115"
     CDS             6825..7577
                     /locus_tag="VFJ72_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7480638.1"
                     /translation="MTAGLLMAVVIGLSLGILGGGGSILTVPVFVYVMGFGVKGAIAM
                     SLALVGVTSLFGAVGHWRSGNVNLRAAAVFGAVAMGGTYLGARVAVFFSGPAQLILFA
                     LVMFAASYVMFRNGRGSGAAGENGRMNLALVMLAGVAVGVLTGLVGVGGGFMIVPALV
                     LLGGVDMKEAVGTSLLVIALNSAAGFVGYLGRVEIHWGVLTAFTLVAITGSLAGSYLV
                     RFIPEKALGRGFSVFLVVMGAFILFENRGAFF"
     gene            complement(7574..7918)
                     /locus_tag="VFJ72_14120"
     CDS             complement(7574..7918)
                     /locus_tag="VFJ72_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7480639.1"
                     /translation="MPEYRKENLSDEAFEMLGERFRALGEPMRLRLIYALMDGEKTVS
                     ELVEETGGLQANISKHLKLLLDAGVVGRRKQGLHAYYRISDPTIFELCDLMCGSIQDQ
                     LESNLGRFSRSS"
     gene            8046..8354
                     /locus_tag="VFJ72_14125"
     CDS             8046..8354
                     /locus_tag="VFJ72_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7480640.1"
                     /translation="MTVPGIGFEEVRERMTGGGVLLVDVREDEEWREGHLPGAVHAPL
                     SEFGKEVSRSFAGREVVVYCRSGRRSARVVERLVRMGHTGVWNLEGGIEAWRGRLVKD
                     "
     gene            8427..9140
                     /locus_tag="VFJ72_14130"
     CDS             8427..9140
                     /locus_tag="VFJ72_14130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7480641.1"
                     /translation="MSLEAEEGLEFDYIFEQFRVPRGCLGYLIADPETRLAAVVDPEL
                     EMVEPVLDAVFRHGLRPAYIIDTHTHADHVSGARELKAKTVAKLVMHEEAPSRSVDIR
                     VEDGDRLHLGKLPLKFLHTPGHAKDLVSVVLPGRILSADALLIGSCGRTDLLNGNATR
                     QYHTLYYTYRALPDDLLVYPGHDYKGRDCSTLGDEKRENGKMNFASEEEFVEYMDLEN
                     PKKLDPVYHLAEALKENMK"
     gene            9168..9584
                     /locus_tag="VFJ72_14135"
     CDS             9168..9584
                     /locus_tag="VFJ72_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HET7480642.1"
                     /translation="MMSQVRERGTYSIQTHLEAVPYDEAVEKVTGALKEEGFGVLSTI
                     DVKSTLKEKLDADFRPYIILGACNPPLAKRALDAELELGLLLPCNVIVYADESGAGSV
                     VSAVEPRAMLGVAQNPELVPIAENVQGRLTRALESL"
     gene            9701..9988
                     /locus_tag="VFJ72_14140"
     CDS             9701..9988
                     /locus_tag="VFJ72_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480643.1"
                     /translation="MTDTRKSARRGVWTPIAVLTPIFLLGLVVLFAAGCGQAQRSSSA
                     EEKQASKPSSEQSAKKPEPAARESRTGGRLEHPALGEKGAPVVMVEYADYQ"
     gene            10001..10453
                     /locus_tag="VFJ72_14145"
                     /pseudo
     CDS             10001..10453
                     /locus_tag="VFJ72_14145"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            10457..11302
                     /locus_tag="VFJ72_14150"
     CDS             10457..11302
                     /locus_tag="VFJ72_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HET7480644.1"
                     /translation="MEVSYLAAFLGGVLSLLSPCSALLLPAFFAYAFGGGGELVKKTG
                     VFYLGLAATLVPLGMGISAVSALFYGQRSLLIMLSGSLLIAFGVMQILGRGFAVGPLE
                     RLRGRIRGDSAGAVFSLGAVYGFAGFCSGPILGAVLTVAASSGGAFQGAALLAVYAAG
                     MVAPLFLMALFWERLDLSRRRWLRGREVSLGRLRFHTTNLISGTMFVLLGVAFIAYEG
                     TSALAGLYESNGASDAAFAAEEWANGFSQSVPAWVMVVVLAAILLGWWVYRRYGRHKK
                     SSASR"
     gene            11429..11809
                     /locus_tag="VFJ72_14155"
     CDS             11429..11809
                     /locus_tag="VFJ72_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480645.1"
                     /translation="MKTQRIPTIERILHRIEDRVEEWRREDAALKAAAEANRDSLWEE
                     ARERERLLRETINEEESARDSLEEITRQQRVVFVAHSEEVARTLEEFAADRDCLVRVV
                     PGRRGSHAREAGIKGSWLVFEPQD"
     gene            11930..12688
                     /locus_tag="VFJ72_14160"
     CDS             11930..12688
                     /locus_tag="VFJ72_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480646.1"
                     /translation="MELLMPGRFEDAATAYGYQRAVFEEVASGVRPPTISITPSTRHV
                     GVTRRDTFRPGFDEAVRSADCLGYPVLVRGAGGGATAADSGTFGFSIIRPTESGGSQS
                     GIQRRYDEVAALALAAFARLGVRAEVGEVRDEFCPGDHSIRIGGHESGMKVVGIAQRL
                     TSRATSVGGIVLVHGEEDLACVLEGVYGAMRLPFRSTSVGSLRRAGSPASVEEVVEAF
                     AAEAELRYGARSVPLGERTLESARRDGGRHLVSA"
BASE COUNT         2373 a         3660 c         4389 g         2473 t
ORIGIN      
        1 tgccgaagaa ggcgacggag atcgcgatcc agttccgccc gaccccacac acgcccttcg
       61 cccacgacga caccaagagc ctcgacccga acgtgctcgt tgtccgggtg cagccggagg
      121 agggcatctc gctcaagatc ggggcgaagg tccccggcca gggcttcgag gtaagctccg
      181 tgaacatgga cctcctctat gggacggcgt tcctcgaaga agccccggac gcctaccagc
      241 ggctcctcct ggacctgatg ctcggcgacc cgacgctgtt tatccgggcg gatgaggcgg
      301 agggagcgtg ggacatcctc gcgccggtga tgcaggcatg gtcggagaag aaagaggtct
      361 cgatgtatgc ggccggcgag tgggggccgg aggacgcgga ggagttgctc cggcacgacg
      421 gacgggagtg gagacggcca tgatcgggga gtcccggcgc gctcccggcg gcggcccgat
      481 gagcgtcgag cagatagagc acgagctggc gcaactccgc atgaacgagg acggtacgct
      541 ggctctgcgt tccagcgtcc tcaacctggt cgtcgttacg gatgaggagt ccgccggcga
      601 ggtgacgcgc gcagtctcca acctcgcggg acgatacccg tcgcgagcta tagtcttgat
      661 ctcagacccg gaaggcgagc gtaacgtgga cgtgcaactc tcggctttct gcaacgtgcg
      721 cggcgggggc ggcgcccagg tctgcgccga gcagatcact attcacgccg aaggcccgcc
      781 cgccgagcac ctcgacagcc tcgccggacc gttactcatc tccgatcttc cagtctttct
      841 ctggtatccg ggtgtgttct cgccccactc gccggagttc gccggaatga ccgatatctc
      901 cgaccgtgtc atcctcgact ccgcccgttc gggagattac gaagcttgtt tgcgtggtat
      961 ccacacgctg ctggagaacc ctgagacccc ggcggtagga gatcttcaat gggtcgggct
     1021 atcgccctgg cgctcactac tagcagacac gttcagcgcc cccgaacggg cgggagaact
     1081 ggagaagata gagcgcgtcg aggtcgtcta cgcccccggc ggcgagtgcc gggctctgct
     1141 gctggcgggc tggctcgcct ctaccctggg ctggagtgtg gagggcgtca accgcgacgg
     1201 agatagtcta caggtcgagt ttgccgggcc gtccggcgac gtatctttcg agatgtcgtc
     1261 cagttctccc gacgcccggc tgcgccgcat ccgcatccac tccgcggagt acagcttcca
     1321 ggtctcgcgc catcgggaga tgtcagacgt ccggacgacc gtgctgcacg gtgacgagtt
     1381 ggttgccgag cggaccgtgc acatcggctc cttcgatctc ggtgtcctgg ttggagagga
     1441 actccagtac cggggccgcg acggagcgta tgaagctgcc ctcggggttg ccgtggagat
     1501 cctggaccat tgagcgtccg ggtctacgag gacaagcagg aactggccga ggccgccgcc
     1561 cgagacttcg ccgagcgcgc ggcggaggct atcgaggacc gaggccggtt caccgttgct
     1621 ttagccggcg gctccacacc gaaggcgacc tacgagatcc tggctcgcga ctacgctgac
     1681 gaagtcgact ggagccgcgt ccacgtcttt ttcggcgacg agaggactgt cccgccggac
     1741 cacgaggact cgaactaccg catggcccgc gaagcgttgt tggatcacgt cccgataggc
     1801 tccgtccatc ggatgcgcgg agaactctcg cccgaagaag ccgctgcctc atacgagaat
     1861 gagttgcgcg agttcttcgg aggtaatctc cccagcctgg acctcgtaat gctcggcatc
     1921 ggggaggacg gtcataccgc gagcctcttc ccgaacaccc cggcgctcga cgtcaccgac
     1981 cactgggtag tcgagaaccc ggtcgagaag ctggacaccg tgcgcctgac gctcacaaag
     2041 ccggttatca acgcggcgcg ggcggtcgtg ttcgcggtcg ccggcgaggg caaggctgag
     2101 gcattgcgcg aaatacttga gggagaagta gacccgcacg attatcctgc gaagttcgta
     2161 caacccgcgg gcggagcggt atggatggtg gatcgctcgg cttcggtccg actctcgtag
     2221 agagcggcct caagccccat tagagcaatc ttctcaaaat cgcgagtttg ggtttccggt
     2281 tactagctta tgggtaacgc ttgccacgac gtgaaagcta cctctctgga ggatagacat
     2341 gaggaagtgg aaggtacggc tgtcggcgct actagccgca accatgatgg cgctcgtctt
     2401 cgcctcgccg gcgatggcgc aggtgaccat cccgcaggtc aacgtgcctc agcaagtaaa
     2461 tcaggcaccc caggttaacg caggcaatct tgtcggagta aacgtcaacg cattgaacaa
     2521 cgtcaatgcc ctaaacaatg tctctgttgt ggtgaacaac gtgctgaaca acaataacgt
     2581 ggccgtgaac gccaacgtcc tgggcggcac caacatagtt gtcttccagt aaggcaaaac
     2641 ccacaagata gacaattttg tagagagacg gggcctttta gccccgtctc tctacgtttg
     2701 tggacgaaca gctacatcct ttacaccggt ttgtttcgaa gtgtgtgttg ctggcgttgt
     2761 gcgttcttct ggaagctatc cggactactc tcgctgtgag tctaatggtt cctgtggctc
     2821 ctgactcggt tttcagggtg taatggttta cacctctcgg ggggtcaaat aggtacgtgc
     2881 atgtgtatat cgacgactta agcgcacgga gagcgggtcg gcgcttcatt gacgtgaaag
     2941 cagctctgaa gagcgacaaa gcgcgatagc cgtgccgacc cgcttgcata tcttacgcta
     3001 acaattgctg gtctggatca cgccaggagc aaaattcccc taaagattac acctttcggc
     3061 atagaaaact agaaaacctt cttcagcagc ccgatggcgc cctgcttcca cggcacccgg
     3121 tgcgagacgc cggaggcgtt ctcgaacttc ttccgattat ccagatacca ccggaagttt
     3181 ctcaagagag cctccttgtt ggagaacttc ggggtgtagc ctagctcgcg ttcggccttc
     3241 tcgatggaga cgaacgagtc tttcgaggcg gtctcgtaga cccacttgta gaggggagag
     3301 acgccgagcc tatctaggac gcgaagaccg agaatggcgg gcagcgccgg gaagccgacg
     3361 accttcttgc cgtgaccggc gtagtcgagg acggcctggt agtcctcttt cattgtagtg
     3421 aactccttcg cgccgatgtt gaaggtgtcg ttgaccttct cttccgggag cgcgagggtg
     3481 agggcaatgg cgtcgcagag gtcttcgaca tctagtagct gatagcggtt gttgccggag
     3541 ccgatcatgg ggaagttgtg gccggtaacg gcccagtcgt agagcagcgc gaagacgccg
     3601 agtcgttcgg ggccgacgaa ggacttgggg cggatgatgg ggacgacgag acctttcgcc
     3661 cggtactcca ggcagatcat ctcggcctgt atcttggcct gtccgtaagg cccgacaccc
     3721 tccagcttgt ccgtctcgta gagcgggtgg tggtccggga tgccgtagac agcggtcgag
     3781 gagacgtgga cgacgcgctt tacgccacgg cgcagtgccg cgtccagcac gtttcgggtc
     3841 ccttctacgt cggtggtgta gatgtcgtcc ggggagtaga gcgggagggc cgccgctgta
     3901 tggacgacga aatccacgcc ctccatcgct cggtcgagga gagccctgtc ccggatgtcg
     3961 ccctttatgg tcgcgacccg gtcgcgctcg gggtagtcga agtcttccac gtccagggaa
     4021 gttacggcgt agccgcggga gaggaggtgt cttgcgaggt tgatccccag gaaccccgct
     4081 ccgccggtta tcagaactct ctgctggtcc atcgaaaaat cgtctccttg cgtactctga
     4141 tctcgaggtc gcggaaaggg tccgcgcaag cggcgaattt tagcacggtg tccggcaatg
     4201 atgggcggag ctgggcactc caggctaatt gaacgcctga tgggcacggt gtttgttggg
     4261 gtacctggag gtgacgcccg gacgctcggc atgctgagtg cccgagccct ccgcgttgtt
     4321 tttgatcaat ccttgtcaaa tgattggacc gtactgctca tccggtagtt tgtcaggagt
     4381 gctaactttt gcgcatatgg agatcggcgg acaatacgac ccattccgga acgcctccag
     4441 ggggcagtct ccgctggcgc tgaggctcgg tgacgcgccc tatggtccgg agtccgggcg
     4501 cgtgggggtg atgaactcgc tggcccgctc ggggctgccg atcgcggagg gcgtggtgct
     4561 gatggaggag tgtcaccgaa gcttcctgga gtcgagcgga ctcgcctggg acctcctggc
     4621 ctgcggagac gggaccgaag tccgcaaatt ggcgcaggcg ctgcgccgaa gatacaggag
     4681 atgtactctg aaggaggagc tgaaaggtgt gataagagcc gcgctcgccg agatcggcgg
     4741 ccgctcggtg gcggtcgtct ctgaggatat gacgcgcacg gggctccgca gcatcccgcg
     4801 cgtcgaggag gcggtcttgc gggcctggct ctcgccgaac ggtctgaggc ggcaggtcgg
     4861 agcggcgatg agaggcggag agatcccgct gtggcccgta ctgatccagc acgagcttca
     4921 tgacggataa tataaggcgc cttccgggaa ggagagcata gtggcgaaac gaaactggag
     4981 cgaattgagc gtcccacaga ggactggcgt tatgctggcg ggtgcggtgc agctcggact
     5041 gctgggagct gcgcttgcgg acatctaccg ccggcccgcg gacgggatac ggggcaacaa
     5101 gtggttgtgg gccgcggctt ccttcgtcaa tttcgttggt ccagtctcat acttcgtctt
     5161 cggacggagg cgttagagag cttttggcat cccttcgaag tgggcgtatg attccagtcg
     5221 aacgcggccg gggatcttgt ttcggaagtc cgttcggagc gcgcgtagag ctcgttcaag
     5281 aggaggtcac caaggttgta ccatagctct cctttcatcc gtttcgcccg gtggacctca
     5341 cgcgccacgg gacaccctgt ctccttcgtc gccgccttga tgatcatagt ggcctgggct
     5401 gtcaccggtc cgatattcgg gttcagcgat acctggcaac tagtcataaa cacgagcacc
     5461 accatcgtca ccttcctgat ggtgtttctc atccagaaca ctcagaaccg ggacagcgaa
     5521 gccatgcagc tcaagctgga cgagttgatc cgggcgatga actgcgcgca caatgccatg
     5581 ctggacctgg aagacctgac ggaggaggac ctgaacaaga tcaagcgcca ctacgagtcg
     5641 ctggctcgca agtccaggga ggacctgaag cggggtctga aggacacggg aactattgaa
     5701 gtgcgcgacg actagcctcg tgcagggatc tttgctcgaa cgtgtgggat tttggatgac
     5761 aggctcccat gatcgtagat agcgccgtct acgtggacgg gcgccggagc gcggcttgtc
     5821 ctctgggcga gatccggcag acctgccgcg agagcggcgg gttcgcctgg gtcgggctgc
     5881 atgacccaag ctccgaagag ttcgactcgg tagcgaggga actcgggttg agcgaggaca
     5941 tgatccggga caccatccgg gagcatctgc gtccgaaggt agagcgctat ggagacctca
     6001 ccttcgttgt gttgcgggcg gcccggtatc tcgaaatacc cgagacggtc gagttcgggg
     6061 aggtcctcgc cttcgtagga ccggatttcg ttgtgacgat ccggcgcggc gaggctccgg
     6121 cgctctctgg attgcggcat cagttggagg gtgagccaca gacgctgcgt cgggggccga
     6181 tggcggtact gtatgccgtc atggaccggg ttgtgagcga ctatggtccg gtggtcgagg
     6241 gtcttgagaa cgacatagac gagatcgaga ccgaggtctt cggcgggaac tccagagtgt
     6301 cgcgcaggat ctacgagctg tcgcgcgagg tgatccagtt ccaccgggcg acccaacctc
     6361 tggcggctgc cctgaaacac ttgagtgata ccgaagacca gtacccggag ttcaagcgtc
     6421 gccttcgcgt tacccaagat cgcctgttac gggcgacgga gcaggtcgag ggtttccgcg
     6481 aactgctcac caacatcctg agcgtcaacc tcacgatggt gagtgtgcgc cagaacgaac
     6541 aggtccagaa ggtcagctcc tgggccgcca tcctggtggt gccgacgatc atcaccggca
     6601 tctacggcat gaactttaga tatatgccgg aactctcctg gagcttcggc taccctttct
     6661 cactccttct catgcttgcc gtgtccggac tcctgtacct cggcttcaaa cgctccggct
     6721 ggctctaatc caccactgcc gtccggctcc ataattatac ttattgacca attggtaata
     6781 tagttatacg atgtggtcat ggacgaggga tgtggagaga ggctgtgact gctggtcttt
     6841 tgatggcggt tgtgatcggg ctctcgctcg ggattcttgg gggtggggga tccatcctca
     6901 cggtcccggt cttcgtgtac gtgatgggtt tcggggtgaa gggggccata gccatgagcc
     6961 tcgcgctggt gggggtgacg agcctgttcg gggcggtggg gcactggagg tccggcaacg
     7021 tgaacttgcg ggccgcggcg gtcttcgggg cggttgcgat gggcgggacg tatctggggg
     7081 cgcgggtggc ggtcttcttc agcggaccgg cgcagttgat cctgttcgcg ttggtgatgt
     7141 ttgccgcctc ctacgtcatg ttccgcaacg gacggggctc tggcgcggcg ggtgagaacg
     7201 ggaggatgaa cctcgcgctg gtcatgctgg ccggggtcgc ggttggggtg ctgacgggac
     7261 tcgtgggcgt gggcggaggg ttcatgatcg tccccgcgct ggtcctgctc gggggggtgg
     7321 atatgaagga agcggtcggc acctctctgc tggtgatcgc cctgaactcc gccgccggct
     7381 tcgtcggata tctcgggcgc gtggagatcc actggggagt cctgaccgcc tttacactgg
     7441 tggcgataac cgggagtctt gccggcagtt acctggtccg tttcatcccg gagaaggcgc
     7501 tcgggcgagg gttctctgtc tttctggtgg taatgggcgc gttcatactg ttcgagaacc
     7561 ggggcgcctt cttttaagag cttctcgaaa acctgccgag gttcgactcc agttgatcct
     7621 ggatggagcc gcacatcaag tcgcacagct cgaatatggt cgggtcggag atccggtagt
     7681 aggcgtgaag gccctgtttc ctgcgcccga cgacccccgc atcgagcaac agcttgagat
     7741 gcttggagat attggcctga agtccgccgg tctcctcgac caactccgag acagtcttct
     7801 ctccatccat cagagcgtag atcaaacgca gccgcatcgg ctcccccagc gcccgaaacc
     7861 tctcccccaa catctcgaaa gcctcgtcac tcaggttttc cttccggtat tctggcatgc
     7921 taaccctcca aaataagata ttaaatgtac atatagtaat agtctaacac cgccacctca
     7981 ctttccttga caccgcatca cactaatata ctatctggtt atgtagtaat aaaaaggagg
     8041 tttgtatgac ggttcctggt atcgggttcg aggaggtccg tgagcggatg actggtggcg
     8101 gggtgttgct ggttgacgtg cgggaggacg aggagtggcg tgagggacac ctccctgggg
     8161 cggttcatgc gccgttgagc gagtttggga aagaggtgtc gcggtcgttt gcggggcggg
     8221 aggtcgtggt ctactgccgg agcgggaggc gcagcgcgcg ggtggtggag cggctcgttc
     8281 ggatggggca caccggggtc tggaacctgg aaggagggat cgaggcctgg agaggacggc
     8341 tggtgaagga ttgagacggg ttgtacggga ggtaaggagg ccgcaagatg gtaagtgata
     8401 cgaggttcag ggttggagag gtcgagatga gtttagaggc ggaagagggg ctggagtttg
     8461 attacatatt cgagcagttc cgggtcccgc gaggttgtct cgggtatctg atcgcggacc
     8521 cggagacgag gctcgccgcc gtggtggacc cggagctgga gatggtggag ccggtactcg
     8581 atgctgtctt cagacacggt ttgaggcccg cctacataat agatacgcac acgcacgcgg
     8641 atcacgtctc cggcgccagg gagctcaagg cgaagaccgt cgcgaagctg gtgatgcacg
     8701 aggaggctcc ttcaagaagc gtggacatcc gggtcgagga cggcgaccgg ctacacctgg
     8761 gcaagctgcc gctcaagttc ctgcatactc cgggccacgc caaagacctc gtctcggtcg
     8821 tgttgccggg ccggatactc agcgcggacg ctctattgat cggctcctgc ggccggacgg
     8881 acctgctgaa cggcaacgcg acccgccagt accataccct ctactacacc taccgcgccc
     8941 tgccggacga tctgctcgtc tatcccggcc acgattacaa gggcagggac tgctccacgc
     9001 tcggcgatga gaagcgcgag aatggaaaga tgaacttcgc ctccgaagaa gagttcgtcg
     9061 agtatatgga cctcgaaaac cccaagaaac tcgatcccgt ctaccacctc gccgaagctc
     9121 tcaaagagaa tatgaagtag aagagagtag aagagaacga ggaggtaatg atgagtcagg
     9181 tacgagaacg aggcacttat agcattcaga cccatctgga agccgtgccc tacgacgagg
     9241 ccgtcgagaa ggttaccgga gccttaaaag aagaagggtt cggcgtcctg agcacgatag
     9301 acgtgaagtc tacgctcaag gagaagctcg acgcggactt tcggccctac atcattctcg
     9361 gggcctgcaa cccgccgctg gcgaagcggg cgctggacgc ggagctggag ctggggctcc
     9421 tgttaccctg caacgtcatc gtctacgcgg atgaaagtgg cgcgggcagc gtcgtctcgg
     9481 cggtcgagcc gcgcgctatg ctcggcgtcg ctcagaaccc cgaactcgtt cctatcgccg
     9541 aaaacgtgca ggggcggctg acgcgggcgc tggagtctct gtaggttcgg ttctccgcgg
     9601 gcttgcctcg ctccgcgggc ttgcctcgaa gacggggtat atgatccagg cgtattaccc
     9661 gtgcgtaagt tccagggtac gccccgaagg aggtagcctg ttgaccgaca cccgaaaatc
     9721 cgcgagaaga ggcgtgtgga ctcccatcgc agtcctgacg ccgatttttt tgttggggct
     9781 cgtcgtcctt ttcgccgctg gttgcggaca ggctcagaga tcatcgtccg cggaggagaa
     9841 acaggcatcg aagccatcct ccgagcagtc agcgaagaag ccggagccgg ccgccaggga
     9901 gagccggacg ggcggccggc tggagcatcc cgcgctcggc gagaaagggg cgccggtcgt
     9961 gatggtcgag tatgccgact atcagtgacc gttctgcggc aagttcgccc gtgaggtgga
    10021 accggaactg gtcaagaagt acgtggatag cggggtgctc cgcatagagt ggcgggactt
    10081 tccttatctg gggcaggaat cggtgaacgc ggcgctggcg gcgcgggccg cgcaggagca
    10141 ggggaagttc tggaagtatc acgacctgct ctacgagaac cagacctcga ccaatagcgg
    10201 cggtttcagc gacgagaagc ttacggaact ggcgcgcaag gcggggctcg acgtaaaaca
    10261 gtttcagaaa gacctgcgga gcggccgata ccaggaggcc gttcagtcgg acttcgagga
    10321 ggggcagcag cgcggcatct cggccacgcc ggcgttcatc gtgaacgacc gggctctcat
    10381 cggtttgcag ccgctggaga ccttcgagca ggcgatagaa gaggagcggc gaaaggccga
    10441 ggacagctcc tagttggtgg aagtctctta tctggcggcg ttccttggcg gcgtgctctc
    10501 gctcctcagt ccctgcagcg ccctgctact gccggcgttc ttcgcctacg ccttcggggg
    10561 cgggggagag cttgtcaaga agaccggcgt gttctacctg gggctcgcgg cgacgctcgt
    10621 gccgctgggg atggggatct ccgcggtcag cgcgctcttc tacggtcagc ggagcctgct
    10681 catcatgctc tcggggagtc tgctcatcgc ttttggtgtc atgcagatac ttgggcgcgg
    10741 tttcgcggtc ggaccgctgg agcggttgcg gggccggata cggggagatt ccgcgggcgc
    10801 cgtcttctcg ctgggggctg tctacgggtt cgcggggttc tgctccggcc ctattttggg
    10861 cgccgtcctg acagtcgccg cctcatccgg aggagccttt cagggggccg cgctcctcgc
    10921 cgtctacgcc gccgggatgg tcgccccgct cttcctgatg gcgctgttct gggagcggct
    10981 ggacctgagc cgtcgccgct ggctccgtgg ccgggaggtc agtctgggaa ggttacggtt
    11041 tcacacgact aacctgatct ccgggacgat gttcgtgctg ctgggcgtgg ctttcatcgc
    11101 ctacgagggg acctcggcgc tcgcgggact ctacgaatcg aacggcgcct cggatgcggc
    11161 gttcgccgca gaagagtggg cgaacgggtt ctcccaaagc gtcccggcgt gggtcatggt
    11221 cgtggtgctg gccgcgatcc tgctcggatg gtgggtctac cgccggtatg gacgccacaa
    11281 gaagtcttcc gcgtcccggt aggctgtgtt ccggtggtat tggtcaatcc tgattataca
    11341 aatagcgcat cctggtcagt aagacgctgc cgctccagat tacagtaaat gcacggcaga
    11401 actaccggaa ctggagagga gcgcacagat gaagacgcag cgtatcccga cgatagagcg
    11461 gatactgcac aggatcgagg accgcgtcga ggagtggcgc agggaggacg ccgccctcaa
    11521 ggccgcagcc gaggcgaaca gggacagcct ctgggaagag gcccgagagc gggagcggtt
    11581 gctccgggag acgataaacg aggaagagtc cgcgcgggac tctctggagg agatcacgcg
    11641 ccagcagcgg gtagttttcg tggcgcattc cgaggaggtc gcccggacgc tggaggagtt
    11701 cgccgccgat agagactgcc tggtgcgggt ggtccccggt cggcgcggca gccacgcgag
    11761 agaggcgggc atcaagggct cctggctggt cttcgaaccc caggactgaa cggccgactg
    11821 ctacagcggg ctcgaaagac acacctccgg gacggactac gagcggccgt cccggtctcc
    11881 gcgctgctag aatccggggg tcagggaccg ttaaggaggg agaggcgcga tggagttgct
    11941 catgcccgga aggttcgagg acgccgccac ggcttacggc taccagcgcg ccgtcttcga
    12001 ggaggtcgcc tccggagtcc gcccgccgac gatctccatc acgccttcca cccggcacgt
    12061 cggggtgacg cgccgcgaca ctttccgtcc cggtttcgat gaggcggtgc gctccgcgga
    12121 ctgtttgggg tatccggtcc tcgtccgcgg cgcgggtggg ggcgcgaccg cggcggatag
    12181 cggcactttc gggttctcga tcatccgtcc gacggagagc ggagggtccc agagcggcat
    12241 ccagagacgc tacgatgagg tcgccgccct ggcgctcgcg gcgttcgcgc ggctgggagt
    12301 gcgggcggag gtcggggagg tccgcgacga gttctgcccc ggagatcaca gcatccgcat
    12361 cggaggccac gagagcggga tgaaggtcgt cggcatcgcg cagcgcctga cgagccgggc
    12421 gacgagcgtc ggcggcatag tgctggtcca cggggaggag gacctggcgt gcgtcctcga
    12481 aggcgtctac ggcgccatgc ggctcccatt ccgaagcacg agcgtcggaa gcctgcgccg
    12541 ggcggggagc cccgcgtcgg tcgaggaggt cgtcgaggcg ttcgccgcgg aggcggagct
    12601 tcgctatggc gcgagatccg tcccgctcgg tgagcggacg ctggagtctg cccgcaggga
    12661 cggtggcagg caccttgtct ccgcatagga gggcctatta tcatgagtct ttgccggaag
    12721 agagggaaag ggtgtccggg ctagagcgga ggatagagag cggcgaggtc atcgtcctgg
    12781 acggggcgac ggggacggag ctggagaggc gcggggtgcc gatggacgac gcggcatggt
    12841 gcgcggccgc gctcgttacg cacccggagg tcgtgcgcga ggtacacgag gacta
//
LOCUS       DASSMB010000246         9876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1705355, whole genome shotgun sequence.
ACCESSION   DASSMB010000246 DASSMB010000000
VERSION     DASSMB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9876
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1705355"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /gene="allB"
                     /locus_tag="VFJ72_14165"
     CDS             <1..1285
                     /gene="allB"
                     /locus_tag="VFJ72_14165"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HET7480647.1"
                     /translation="GISDGVVAAVEPELEGSAGMEIDASGLHVFPGLIDAHVHFNEPG
                     RTHWEGFETGSRAFAAGGATTVFDMPLNAYPPTVDGKSFDLKLAAAEKSALVDFALWG
                     AIQPDNLGGMEELAGRGVVGFKAFMSASGTEDFKNVDDLELYEGMVQAERLGLLVGVH
                     AESQPITSRLTQRALAGVRVSARDYLDSRPVIAELEAISRAILFAEDTGCALHIVHVS
                     TGRGVDLVNDARERGVDVTCETCAHYLMLTEDDLEELGAVAKCAPPLRTAEEREALWE
                     RILDGRIPMVTSDHSPCPPEMKSGNDMFRAWGGISGGQSTMQVLLTGGEERGVLLPTL
                     ASVTSEAVAGRFGLSKKGRIEAGYDADLTLVDLDHSGTFKAEDLLYRHKQSPYVGREL
                     KGKVVHTLLRGNPVFSEGRVVSEPKGRLVRPDVPA"
     gene            1418..2857
                     /locus_tag="VFJ72_14170"
     CDS             1418..2857
                     /locus_tag="VFJ72_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HET7480648.1"
                     /translation="MSERPLPASYSDRLYNEDLAPVTERTWTGYSLFAMWMADVHSIG
                     GYTFAAGLFFLGLAGWEVFLALLAGITAVYFLMNLTGLAGQRNGIPYPVLARISFGVF
                     GANIPALIRAIIAIFWYGIQTWLASVAVVILLLRIFPGLEPYTQNSILGLSTLGWICF
                     LALWGAQLFVLRHGMETVRRFVDWAGPAIYVAMFALAIWILIEAGGKIDFSLGSKDLS
                     AGGAVLAFFTAASLVVAYFSTLLLNFCDFSRFSPSERMVKVGNFWGLPVNFTAFSLVT
                     VIVTAGSVAVYGKAITDPVQLVAKIPNTFVLIVGAVTFAVATVGINIVANFVSPAYDL
                     SNVAPKYIDFKRGGLITALLALLVTPWNIYSSPVAVNYFLGGLGAFLGPLFGILMVDY
                     YLVRRGQVDVEALYRAGESEPYWYQGGVNRLAVGVFVISAAVAALIALVPAFSALAPF
                     SWFVGAGLSGAMYWALARRGVERTPETAA"
     gene            2854..4080
                     /locus_tag="VFJ72_14175"
     CDS             2854..4080
                     /locus_tag="VFJ72_14175"
                     /inference="COORDINATES: protein motif:HMM:NF006775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HET7480649.1"
                     /translation="MKDTAATVMDRCDLLANISEESGLIVRPFGSQAMREANDIVSGW
                     MRGAGMSVRRDEIGNLIGRYEGSGDKTLILGSHLDTVRDAGRYDGILGVMASIACVQQ
                     LHNRDERLPFAIEVVAFADEEGLRFGTTFLGSSVFAGAFDRKRLSLKDADGVSIEDAV
                     RAFGGDPCALEVSGRDADDLIGYFEVHIEQGPVLEELDLPVGVVTAIAGQSRVRAGFT
                     GKAGHAGTVPMQSRKDALCAAAELVLEVERAAKAESGAVGTVGQISALPGAGNVVPGE
                     AQLSLDIRHQDDAARVRLRDHLEVRTGEIAAARGCEHEWQLRQETPAVPADEKLSAAL
                     EAAVEGAGIKVHRLPSGAGHDAAQMADLAPIAMLFVRCEEGISHNPAESVTKEDVTVA
                     LDVMGRFLESVAEKEE"
     gene            4105..4518
                     /locus_tag="VFJ72_14180"
     CDS             4105..4518
                     /locus_tag="VFJ72_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HET7480650.1"
                     /translation="MSDVKITAGPYEFLARWERERCPKTVEAFEKLLPYRQKIIHVRW
                     SGEACWIPLGDYDLGVPWEDATSVPQAGEILFYPGGYSETEILFPYYGTVFKSKLGEL
                     AANHLMTITEGRENLRALGELCLWEGAQDILFERA"
     gene            4606..5391
                     /locus_tag="VFJ72_14185"
     CDS             4606..5391
                     /locus_tag="VFJ72_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7480651.1"
                     /translation="MAETTASKSVPQAGGVQSLERALDILEVLGHSEGELGVSEIGMA
                     VGLANGTVHRLLSTLTQRGYARQVSGNRKYTLGPKAITLASSARERLGPLARPFLREL
                     MEVSQESANLAALDRNSVVYLEQVAAPRMVRMFTEPGNRVPPHASGTGKVLLAYQPPE
                     AVTAILGRGGLTRFTPNTITDINRLREELDHIREQGYATDSEEIEEGVRCVAAPVSGA
                     EGKVVAAISISGPAGRLDDGRLEEIVPHIKRIAQDFSKSLSGA"
     gene            complement(5388..6122)
                     /locus_tag="VFJ72_14190"
     CDS             complement(5388..6122)
                     /locus_tag="VFJ72_14190"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET7480652.1"
                     /translation="MKIRQTAVWLSLGILGLLAACGPEDRPRAPDTTSESASKSGTAS
                     READAALISAAQRGDVGAVERLLDEGASVEARDDGGRTALVAASYGNHLGVAKTLIEA
                     GADINSKDDTEQSAYLISTAEVGDDPRLLDLTLENGADVRSLDSYNGTGLIRAADRGY
                     VRVVERLLETDTDIDHVNDLGWTALLEAIILGDGDARHTEVVRTLVEAGADVNIADRD
                     GVTPLQHARERGYGAMVEILRDTGAR"
     gene            complement(6154..7482)
                     /locus_tag="VFJ72_14195"
     CDS             complement(6154..7482)
                     /locus_tag="VFJ72_14195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7480653.1"
                     /translation="MAGKKAILLILLLWALSCAACEAAAERSIEQEVNQPSTSAKGPA
                     NDERSDALGGSATGSADRGRDGIEADLVFTGGTLVDGTGGRPLADAAVAVRDGRIVAV
                     GKADKVTVADGARTLDVSGKTVLPGFINAHVHSRYVGLEETRAWTRAGVTTVRDLGGP
                     LEEMVGRKRDSEASGDPRLPGLLVSGPMISVPGGHPFAIYGDDYPALAVRGPDGARTQ
                     VDRLLDAGVDHIKFAVSGRTETGWPELSDEEIQAIADTAHARGARVSAHVDAAAGLRR
                     AVENGIDDAAHSPRDTIPDDLIREMVERDVALIPTIDVYQNIAEEGGTVAAWNAATLP
                     VVQDNLRRFVAAGGTVALGDDFGNPGVEIGMPMDEIEQWTAAGLDPMRIIVAGTRDAA
                     KVSGVEKELGTVERGKTADLLVVSGDPLKDPRSLERVELVVHEGRLAFRR"
     gene            7694..8506
                     /locus_tag="VFJ72_14200"
     CDS             7694..8506
                     /locus_tag="VFJ72_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7480654.1"
                     /translation="MRFSANVSILFKESPFLERFGRAAEAGFSAVEFWWPAGEDLGKV
                     EKAIKDAGLRVALFNFDAGDMPGGERGLVGDPDRREQFRENVPVALDLARSLGCGRMN
                     ILAGHEKEGMSREEQLDLARENVAFAADAAKDAGVEVVVEAVNTFENGPYLLYRTEQA
                     VEFVRSVGRENVKIQHDFYHMQRMEGNLVATLREHIGVIGHVQIADSPGRGEPGTGEI
                     HYSYVLGELEKLGYDGYVGLEYNPSEGDTEASFWWLPKELRGGDVSVEDLKL"
     gene            8522..9409
                     /locus_tag="VFJ72_14205"
     CDS             8522..9409
                     /locus_tag="VFJ72_14205"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HET7480655.1"
                     /translation="MAEKIGFIGLGIMGKPMAKNLLEAGYELTVYNRSPEKAEELAGD
                     GATVGGNPKEVAENSDIIITMLPDSPQVNEVVAGENGVLEGIKEGALIVDMSTISPVV
                     TEELAAKVKEKGASMLDAPVSGGDVGAIEGTLSIMVGGDENDFERAKPLFDVMGKTIT
                     HVGPTGAGQVAKAANQIVVALTIEAVSEALVLGSKGGVSPEKILDVLGGGLAGNKVME
                     VKREKFLSHTFDPGFRAELHHKDLGIALAAGREYGVALPVTAIVDQMLLAMRKKGWGG
                     EDHSALLRIIEDLSQHEIG"
     gene            9463..>9876
                     /locus_tag="VFJ72_14210"
     CDS             9463..>9876
                     /locus_tag="VFJ72_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7480656.1"
                     /translation="MARMDVMDAVVKVMEDEGVEVAFGVPGAAILPLYKSLSKSEQIK
                     HYSVRHEEGGTHAADGYTRVTGKVGINIGTSGPAGTNMITGLYTCMADSIPQICITGQ
                     APTNVLHKEAFQAVDIVEIAKPVTKWAVQVREPAQL"
BASE COUNT         1715 a         2968 c         3448 g         1745 t
ORIGIN      
        1 ggggatctcc gacggcgtgg tggccgcggt cgagccggag ttggaaggct ccgccggtat
       61 ggagattgac gcttccgggt tgcatgtgtt ccctggcctc atagacgcgc acgttcactt
      121 caacgagccg ggacggaccc attgggaggg gtttgagacc gggtcgcggg cgttcgcggc
      181 gggtggggcc acgaccgtct tcgacatgcc gctcaacgcc tatccgccga ccgtggacgg
      241 gaagagcttc gacctcaagc tggctgcggc ggaaaagtct gcgctcgtgg acttcgcgct
      301 ctggggagct atccagccgg acaacctcgg cggcatggag gagctagccg ggcgcggcgt
      361 cgtggggttc aaggcgttca tgtcggcgag cggcaccgag gacttcaaga acgtggacga
      421 ccttgagctg tacgagggga tggtccaggc cgagcggctg gggttgctgg tgggtgtgca
      481 tgcggagagc cagccgatca cgagccgcct gacccagcgt gcgctggccg gggtccgggt
      541 ctccgcgagg gactatctgg attcccgtcc ggtcatcgcg gaactggagg cgatcagccg
      601 cgccatcctc ttcgcggagg ataccggctg cgcgctgcac atcgtccacg tgagcaccgg
      661 acgcggagtg gacctcgtga atgacgcccg cgagcgcggc gtggacgtta cgtgcgagac
      721 ctgcgcgcac tacctgatgc tgacggagga cgaccttgag gagctcggag ccgtcgccaa
      781 gtgcgccccg ccgctgcgaa cggctgagga acgggaggcg ttatgggagc gtatcctcga
      841 tggtcgcata ccgatggtta cgtcggatca ctcaccctgt cctccggaga tgaagtccgg
      901 gaacgacatg ttccgggcgt ggggaggtat ctcgggtggt cagtccacca tgcaggtgct
      961 gctaaccggg ggtgaggagc gtggagtgct gcttccgacg ttggcttcgg ttacatcgga
     1021 ggctgtcgcc gggcggttcg gactatcgaa gaagggacgg atcgaagctg gatacgatgc
     1081 tgatctcacg ttggttgacc tggaccacag cgggactttt aaagcagagg acctgctcta
     1141 cagacacaaa caaagtcctt acgtcggcag agagctaaag gggaaagtcg tacatacgct
     1201 gcttcgcggt aatccggtct tttcagaagg gagggtagtt tcggagccga aggggcggct
     1261 ggtgagacct gatgtcccgg catgacatac ggcattgatt ggcggtttgg gtcagatgaa
     1321 gagcggtggg cgaacggact aaatgcgtag aagggatatc tcttagaggt acgaatcttg
     1381 atggtgggag tcaagcaagg gaaaggaaga tagagtcatg agtgagagac cgttgccagc
     1441 gagctacagc gatcgcctct ataacgagga tctcgcgccg gtcacggagc gtacatggac
     1501 ggggtacagc ctcttcgcca tgtggatggc cgacgtacac agcatcggtg gatacacgtt
     1561 cgcggcgggg ctgttctttc tgggtcttgc gggctgggaa gtattcctgg cgctgctggc
     1621 cggaataacg gccgtctact tcctgatgaa cctcacgggg cttgcggggc agaggaacgg
     1681 gataccgtat ccggtgctgg cgaggatcag cttcggggtc ttcggggcca acataccggc
     1741 tcttatccgg gcgatcatcg ccatcttctg gtatgggata cagacctggc tcgcgtccgt
     1801 tgcggtggtc atactgttgc tcaggatctt tccggggctc gaaccctata cgcagaactc
     1861 tatcctggga ctctcgactc tgggttggat ctgcttcctc gctctctggg gcgcgcagct
     1921 atttgtcttg cggcacggga tggagaccgt ccgccggttc gtggattggg cggggccggc
     1981 gatctacgtc gccatgttcg cgctggctat ctggatcctg atcgaagccg gcggcaagat
     2041 agacttcagc ctgggctcga aggacctctc cgccggagga gcagtcctgg cgttctttac
     2101 cgccgcctct ctggtcgtgg cgtacttctc gacgctccta ctgaacttct gtgacttctc
     2161 gcggttctcg ccctcggagc ggatggtcaa ggtcggaaac ttctggggcc tgccggtgaa
     2221 cttcacggct ttctcgctgg ttaccgtcat cgtgacggcg gggtcggtgg ccgtctacgg
     2281 gaaggccatc acggacccgg tccagctagt ggcgaagatc ccgaacacat tcgtcctgat
     2341 cgttggcgcg gttacgttcg cggtcgcaac ggtggggatc aacatagtgg cgaacttcgt
     2401 ctcgcccgcg tatgacctct cgaacgtcgc cccgaagtac atagacttca agcgtggcgg
     2461 attgatcacc gccctcctcg ccctcctcgt gacgccctgg aacatctaca gcagccccgt
     2521 cgccgtgaac tacttcctcg gcggtctggg cgcgttcctc ggcccgctct tcggcatcct
     2581 catggtcgac tattacctcg tccggcgagg ccaggtagac gtggaggctc tatatcgggc
     2641 gggagagtcc gagccgtact ggtatcaggg gggcgtgaac cgtctggcgg tcggggtgtt
     2701 cgtgatctcc gcggcggtgg ccgcgctcat cgcgctggtc ccggcgttca gcgccctggc
     2761 tcctttctca tggttcgtgg gggccggtct ctcgggggcc atgtactggg cgctggcgcg
     2821 gcgcggcgtg gaacgtaccc cggagacggc ggcttgaaag ataccgcggc gacggttatg
     2881 gaccgctgcg acctgctcgc caacatcagt gaggaatccg gcctgatagt gcgtcccttc
     2941 gggtcgcagg cgatgcggga ggcgaacgac atcgtctccg gctggatgcg tggcgccgga
     3001 atgagcgttc ggcgcgatga gatcggcaac ctcatcggcc gctacgaggg gtccggggac
     3061 aagactctta tcctcggctc ccacctagac accgtccgcg acgccggcag gtacgacggc
     3121 atcctgggcg ttatggcctc catcgcctgc gtccagcaac tgcacaaccg cgacgagcgg
     3181 ctgcccttcg ccatagaagt ggtcgccttc gcggacgagg agggcctgcg cttcggcacg
     3241 accttcctgg gcagttccgt cttcgccgga gcgttcgacc ggaagcgtct gagcctcaag
     3301 gatgccgacg gcgtcagcat cgaagacgcg gtccgcgcct tcggcggaga cccctgcgcc
     3361 ctggaggtaa gtggccggga cgcggacgac ctgatcgggt acttcgaggt ccacatagag
     3421 cagggtccgg tgctggaaga actggacctg cccgtcggcg tagtcaccgc catcgcgggc
     3481 cagagccgcg tgcgggccgg cttcaccggc aaggccggac acgccgggac cgttccgatg
     3541 cagagccgta aagacgccct gtgcgcggcg gcggagctgg tcctcgaagt cgagcgagca
     3601 gcgaaggcgg agagcggcgc cgtgggcacg gtcggccaga tatccgcgct ccctggagcg
     3661 ggtaacgtcg tgcccggcga ggcgcaactc tcgctcgaca tccgccatca ggacgacgcg
     3721 gcgcgtgtgc ggctgcgcga ccatctcgaa gtcaggacgg gcgagatagc cgccgcccgc
     3781 ggctgcgagc atgagtggca actgcgccag gagaccccgg cggtccccgc ggacgagaag
     3841 ctcagcgcgg cgctcgaagc ggctgtcgag ggcgcaggta taaaggtgca ccggctgccc
     3901 agcggcgcgg gccatgacgc ggcgcagatg gcggatctcg ccccgatagc catgctcttc
     3961 gtgcgctgcg aggaggggat cagccacaac cccgccgagt cggtcacgaa ggaggatgtg
     4021 accgtagcgc tggatgtgat gggccggttc ctggagtcgg tcgccgaaaa agaggaatag
     4081 aatccgagct agaaggagtt acaggtgagc gacgtgaaga taacggccgg gccctatgag
     4141 tttttggcgc ggtgggagag ggagcggtgt ccgaagaccg tggaggcgtt cgagaagctg
     4201 ctgccttacc ggcagaagat catccacgta aggtggagcg gggaagcttg ctggattcct
     4261 ctgggcgact acgacctcgg ggtcccgtgg gaggacgcca cgagcgtgcc gcaggccggg
     4321 gagatcctgt tctaccccgg aggctactcg gagacggaga tcctgtttcc ctattacggc
     4381 acggtcttta agagcaagct gggcgagctc gccgcgaatc acctcatgac gatcaccgag
     4441 ggccgcgaga accttcgcgc gcttggagaa ttgtgcctct gggaaggcgc tcaagacata
     4501 ctcttcgaac gcgcctgatt cacccgagta catcgcgtcc gctagggtat actctgcgaa
     4561 gcattccgat atagggaaga gatattctag aaagcgggtg agtctgtggc agagacaacg
     4621 gcaagcaagt cggttccgca ggctggcggg gttcagtctc tggagcgggc gctggacatc
     4681 ctggaggtgc tgggccattc agaaggcgag ttgggggtca gcgagatcgg tatggccgtt
     4741 gggctggcga acggcacggt tcaccggctg ctgtcgacgc tgacacagcg agggtatgcc
     4801 cgccaggtct cgggcaacag gaagtacacc ctgggtccga aggccataac gctcgcctcg
     4861 tcggcgaggg agaggttggg tccgctggct cggccgtttc tcagggagtt gatggaggtg
     4921 agccaggagt cggccaatct ggccgcgctg gacaggaact cggtcgtcta cctcgagcag
     4981 gtggcggcgc cgcgcatggt ccgcatgttc acggagcccg gcaaccgggt cccgccgcac
     5041 gccagcggca cggggaaagt gctgctcgcc taccagccgc cggaggctgt aacggcgatc
     5101 ctgggccgcg ggggtctgac ccgctttacg cccaacacca tcaccgacat taatcggctg
     5161 cgggaagagc tggatcacat ccgggagcag ggctacgcga cggactccga ggagatcgag
     5221 gagggcgtgc gctgcgtcgc cgcgcccgtc tccggcgccg agggcaaggt ggtcgcggcc
     5281 atcagcatct cggggccggc ggggaggctc gacgacggga ggcttgagga gatcgtgccg
     5341 cacatcaagc gcatagcgca agacttctcg aagagcctga gcggcgccta gcgcgctccg
     5401 gtgtcgcgca gtatctcgac catcgcgccg tagccgcgct cgcgggcgtg ctggagcggc
     5461 gtgaccccgt cccggtcggc gatgttcacg tctgcgccgg cttcgacgag cgttcgtacc
     5521 acctccgtgt gtcgggcgtc gccatctccg aggatgatag cttcgagcaa ggccgtccat
     5581 cccaggtcgt tgacgtggtc tatatccgtg tcggtttcga gtaacctctc gacgacgcgc
     5641 acgtagccgc ggtccgccgc ccggatcagg ccggtcccgt tgtaggagtc gaggctgcgg
     5701 acgtctgcgc cgttttcgag ggtgagatcc agcaggcgag ggtcgtcgcc gacttcggcg
     5761 gtcgagatca ggtacgcgct ctgctcggtg tcgtccttgg agtttatgtc cgccccggct
     5821 tcgatgagcg tcttcgccac gcccaggtgg ttcccgtagg atgcggcgac cagggccgtc
     5881 cggccgccgt cgtcgcgggc ctccacgctc gcgccctcgt ccagaagccg ctcgacggct
     5941 cccacgtcgc cgcgttgggc ggccgagatc agggcggcgt ccgcttctct actggctgtg
     6001 ccgcttttcg aggcggactc cgatgtcgtg tcgggtgcac ggggccggtc ctccgggccg
     6061 cacgcggcca gcaatcccag gatgccgagg cttagccaga cggcggtctg ccggatcttc
     6121 atgcctcact gcctttctgt ctcgcggtct tttctaccgg cggaaggcga gtctgccttc
     6181 gtggacgacc agctccacgc gctccagcga gcgagggtcc ttgagcgggt cgccgctcac
     6241 gacgagcagg tctgcggtct tgcctctctc caccgtgccc agctctttct ctacgccgct
     6301 cactttcgcc gcgtcgcggg ttccggcgac gattatccgc atcgggtcca ggccggcggc
     6361 ggtccactgc tcgatctcgt ccatcggcat cccgatctcg actccggggt tgccgaagtc
     6421 gtcgccgagc gcgaccgtcc cgccggccgc gacgaaccgg cgcaggttat cctgcacgac
     6481 ggggagggtc gccgcgttcc acgccgccac cgtcccgcct tcctcggcga tgttctggta
     6541 gacgtctatc gtcgggatga gcgccacgtc gcgttcgacc atctcccgga taaggtcgtc
     6601 cgggatggtg tcgcggggcg aatgggcggc gtcgtctatg ccgttctcga cggcccgccg
     6661 cagcccggcg gccgcgtcca cgtgcgcgct gacccgcgcg ccccgcgcgt gggcggtatc
     6721 ggctatggcc tggatctcct cgtccgaaag ctcgggccac ccggtctccg tcctcccgct
     6781 cacggcgaac ttgatgtggt ccacgccggc gtcgagcagc cggtctacct gggttcgggc
     6841 gccatcggga ccgcgcaccg cgagcgccgg gtagtcatcg ccgtagatag cgaacgggtg
     6901 cccgcccgga acgctgatca tcggccccga gaccagcaac cccggcagtc gcgggtcccc
     6961 actcgcctcc gagtcgcgct tgcgcccgac catctcctcc aggggaccgc cgaggtcccg
     7021 gaccgtcgtg acccccgcgc gcgtccacgc cctcgtctcc tccaggccga cgtaccggct
     7081 gtggacgtgg gcgttgataa agccgggcag tacggtcttg ccgctcacgt ccagcgtccg
     7141 ggccccgtcc gcgacggtca ccttatccgc cttgccgacg gcgacgatcc ggccgtcacg
     7201 aacagccacc gcggcatcgg ccagcggccg tccgccggtc ccgtccacca gcgtgccgcc
     7261 ggtaaacacg aggtccgcct cgatgccatc ccgcccacgg tccgccgaac ccgtcgccga
     7321 acctccgaga gcatccgacc gctcgtcgtt cgccggacct ttcgcggatg ttgagggttg
     7381 atttacttct tgttcgatgg accgttcggc cgccgcttcg cacgccgcgc acgagagcgc
     7441 ccatagaagc agaataagca ggatcgcctt cttgccagcc acatctttca attacgtgcc
     7501 tcgccgcccc gccggacgac gattgccgaa gcctaggggc tcatacgttc ctgtccatgc
     7561 ccgcgggatc tcacccgaca gcgacaggca ccgtatttcg ctcttgacat cgttcatgtc
     7621 ctcattctat actgagttcc gcgaaatgaa atataaattc tgaataatgg tagaaaatgg
     7681 aaaggtggca gagatgaggt tcagcgcgaa tgtttctatc ctgttcaagg agtcgccgtt
     7741 tctggagcgt ttcgggcggg cggcggaggc cgggttctcg gcggtggagt tctggtggcc
     7801 ggcgggggag gatctcggca aggtggagaa ggctataaag gacgctggtc tccgcgttgc
     7861 gctcttcaac ttcgacgcgg gggacatgcc gggtggtgag cgggggctgg ttggcgaccc
     7921 ggaccgccgg gagcagttcc gggagaacgt tccggtggcc ctggatctcg cccgctcgct
     7981 cggttgcggg cggatgaaca tactcgccgg acacgagaaa gaagggatga gccgcgagga
     8041 gcagttggac ctcgcccgcg agaacgtcgc gttcgcggcg gacgcggcga aggatgccgg
     8101 ggtcgaggta gtggtggagg cggtcaacac gttcgagaat gggccgtacc ttctgtaccg
     8161 gacggagcag gccgtggagt tcgtgcggag cgtgggccgg gagaacgtca agatacagca
     8221 cgacttctac cacatgcaga ggatggaggg gaatctggtg gcgacgctgc gggagcacat
     8281 cggcgtgatc gggcacgtcc agatagcgga ctctcccgga cgcggggagc ctggtaccgg
     8341 ggagatccac tactcctacg ttctcggcga gctggagaag ctcggatacg acggctacgt
     8401 cggcctggag tacaacccgt ccgaaggcga cacggaggcg agcttctggt ggctcccgaa
     8461 ggagttgcgc ggcggcgacg ttagcgtaga ggacctcaag ctctagaagg aggagtcagg
     8521 catggcggag aagatcggat tcataggact cgggatcatg ggcaagccca tggcgaagaa
     8581 cctgctggaa gccggctacg agctgaccgt ctacaaccgc agcccggaga aggccgaaga
     8641 gctcgccgga gacggcgcga cggtgggtgg caaccccaaa gaggtcgccg aaaacagcga
     8701 catcatcatc accatgctcc cggactcgcc gcaggttaac gaggtagtcg ctggagagaa
     8761 cggtgttctc gaaggcatca aagagggcgc cctgatcgtg gacatgagca ccatatctcc
     8821 cgtcgtcacc gaagagcttg cggccaaagt gaaggagaag ggcgcgagta tgctggatgc
     8881 gccggtctcc ggcggtgacg tgggggccat cgagggcacg ctctccatca tggtcggcgg
     8941 cgacgagaac gacttcgagc gggcgaaacc tctgttcgat gtgatgggca agaccatcac
     9001 gcacgtcggg ccaaccgggg cggggcaggt cgccaaggcg gcaaaccaga tcgtggtggc
     9061 gctgacgata gaggcggtct ccgaggcgct ggtgttggga tcgaagggcg gggtatcgcc
     9121 ggagaagatc ctggacgtcc tgggaggggg gctggcgggc aacaaggtga tggaggtgaa
     9181 gcgggagaag ttcctctccc acaccttcga ccccggtttc cgcgccgagc tccaccacaa
     9241 ggacctgggg atcgcgctgg cggcgggccg ggagtatggt gttgcgctac ccgttacggc
     9301 gatagtagac cagatgctac tcgcgatgcg gaagaagggc tggggcggcg aggaccactc
     9361 ggcgctgcta cggatcatcg aggacctgtc gcagcacgag atcggctagt tcggacagag
     9421 caggggaagc ccgcaggggc cacgggaaag aggagaaaaa gcatggcgcg gatggacgtg
     9481 atggacgcgg tcgttaaggt gatggaggat gagggcgtag aggtcgcctt cggggtgccg
     9541 ggggcggcga tactgccgct gtacaaatca cttagcaagt ccgaacagat aaagcattac
     9601 tcggtccggc acgaggaggg cggcacgcac gccgccgacg ggtacacgcg ggttacgggg
     9661 aaagtcggga tcaacatagg gactagcggc ccggctggga cgaacatgat caccggcctc
     9721 tacacctgca tggcggactc gataccccag atctgcatca cgggacaggc ccccacgaac
     9781 gtgctgcaca aggaggcgtt ccaggcggtt gacatagtcg agatcgccaa gcccgtgacc
     9841 aagtgggcgg tgcaggtgag ggagccggcg cagctc
//
LOCUS       DASSMB010000247         8415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_196369, whole genome shotgun sequence.
ACCESSION   DASSMB010000247 DASSMB010000000
VERSION     DASSMB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8415
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196369"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1081
                     /locus_tag="VFJ72_14215"
     CDS             200..1081
                     /locus_tag="VFJ72_14215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7480657.1"
                     /translation="MRIGLQVPRFTWPDGTEGLGRKLAEIGRTADSAGFDSLWVMDHF
                     FQIAMVGEAEEPMLEGYSALSYLAGVTERVKLGTLVTGVHYRYPGILAKTATTLDVLS
                     GGRAYLGIGAGWYERESAGLGVPFPTTSERFERLEEALQITRQMWSDEVATYEGKHYR
                     LAETLNSPQPVSRPHPPIMIGGSGEKKTLRLVALYADACNLFAYGGPETIRHKLDVLR
                     GHCEDVGRDYEEIEKTALGTANLAPDGMTADDVISMCRELNGAGIQHLIFNMPNVHDI
                     EPLERFGEEVIPAVREF"
     gene            complement(1068..1643)
                     /locus_tag="VFJ72_14220"
     CDS             complement(1068..1643)
                     /locus_tag="VFJ72_14220"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7480658.1"
                     /translation="MRGAEVLRDLPRLETDRLVLRKMRLEDADAMFDYASDPEVTRYV
                     IWPTHRTMEDSRAFLELTVSNYATGEGFDWGIVYKGDEKFIGTCGFVSRSPEHARAEV
                     GYVISRRYWGRGLTAEALRAIIRFGFERMELNRIEARCIAGNTASARVLEKAGMTCEG
                     TLRERERIQGKLRDMKMYSILKREYLAARTP"
     gene            complement(1640..1888)
                     /locus_tag="VFJ72_14225"
     CDS             complement(1640..1888)
                     /locus_tag="VFJ72_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480659.1"
                     /translation="MESRDARNRRQGLDEGAPRIPDVLARGKFVSVSARIGNDLMGHV
                     CDWNETGLLLDAQDPSGDATGHEFLPWHSIERVSIEER"
     gene            complement(1958..2716)
                     /locus_tag="VFJ72_14230"
     CDS             complement(1958..2716)
                     /locus_tag="VFJ72_14230"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET7480660.1"
                     /translation="MRRLTVLLAAVVLALCLAGSVPAGAASSADTLLGVRFGDHGTYE
                     RAVLDLGMGKSPAQFVPYYEWDRINGDTVVRIELPSVDSTMATGGKGLGLSISRYYTV
                     RAMDGDHLFVDLLLKNNAGPVTVFYLNDPARIVVDVPVNGASSYPPAATGERAVVMRP
                     RAGFSAGPGEVRVKGYARPFEGQGRWRIKNSAGKAVSEGSYRTTDWSTTWGRYRFTAS
                     YPDSLSGEEGVLQVGENSARDGSFQGVSVPLAFR"
     gene            complement(2785..3360)
                     /locus_tag="VFJ72_14235"
     CDS             complement(2785..3360)
                     /locus_tag="VFJ72_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480661.1"
                     /translation="MKRILGVDVDSTIWDVESKVREAVLRVTGDELDSGSVSTWTHLL
                     DTYGEETTTRIFDMVLAPERILEREPYPGAREVLRSFQERHDLSVHFVTRHPFPGDIA
                     PALRSWLKENFGPEVGLSVTPAEKLGVLRELGAFGMIDDRPDTLAAVADAGLWTAAKV
                     QPWNRVFLEGRPDILRFMDWREVPGLLPDAG"
     gene            complement(3357..4016)
                     /locus_tag="VFJ72_14240"
     CDS             complement(3357..4016)
                     /locus_tag="VFJ72_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480662.1"
                     /translation="MAEFGRELTLEDLPRPPLFGLAHPDGRYGRAEAEVGGQPATVAA
                     LFADRELAGEFSSGAAEQGLSGLAGLDPRELSGWRDVEEYALSGPDYVLVVSPEGAGL
                     FHAGDVARVAAEKAGEIPLPLYVISDPGGEAPLVSVETGEGMVLVAVLFSAPERARAF
                     RERAAHLDLPDRLAEIDDADGLRRHALVARRAGASHAVIDPEFGSVEAMPVEDLISWR
                     P"
     gene            complement(4009..5529)
                     /locus_tag="VFJ72_14245"
     CDS             complement(4009..5529)
                     /locus_tag="VFJ72_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET7480663.1"
                     /translation="MGLSRRRKALITLATLIGTFLAALDSTVVGTAMPTVIGDLGGLS
                     LYSWVFAAYLLAATVTGPIFGTLSDTYGRKPVYLAGIFLFLLGSVLAGTAESMTGLIA
                     FRTLQGLGAGAVQPVAITIVGDIFELETRARIQGLFGAVWGISAVVGPAAGGLITDYI
                     SWRWVFFVNVPFGVIAAVLLTLTLRESFEHRSRGMDYLGTALLAGGLISVLFALLGDG
                     GAPTLSAATLAFFFGGLATLALFVVVESRVDDPVVPLGLFSERLFAVAALGNLAAGGV
                     LLGVSVYVPLFVQGSLGGTALTAGAVVAPLSIGWPVGSFVGGRMLLRTGYRTTLLLGA
                     VLIAAGAALCVPMNAGTSLAYVVFAVVVIGLGLGFTSTSYLVAVQNAVPWRRRGIATS
                     SIVFFRTIGGSVGVAVMGALLNYSLGDRYRAAVERASGGDSSLTRLLSDPNALLQPAV
                     RSRIPESAYKQLAGALAAALSPAFLVLLVFGVATVAIAALFPRGGAKDLVSREEHG"
     gene            complement(5653..6021)
                     /locus_tag="VFJ72_14250"
     CDS             complement(5653..6021)
                     /locus_tag="VFJ72_14250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7480664.1"
                     /translation="MLADDDLAALQTSTDALTGAGFKVVSATDGQRALQEALGRRVDL
                     IIMDVSIPHVGGIEACHCLKAMPKTQKIPIVLTAAKKDPAAKAIADRTHGSVRVLRKP
                     FTSEDLVSLARQLVRPKSLI"
     gene            6166..7398
                     /locus_tag="VFJ72_14255"
     CDS             6166..7398
                     /locus_tag="VFJ72_14255"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET7480665.1"
                     /translation="MSVQQEKYFRRGFKLRGNVQHLLDRDYRSEVVDRVRENGNVFTA
                     DGLTLRLADEFGFCYGVDRAVDYAFQTREKFPDKNIYITGDMIHNPSMNDRLREMGIQ
                     FLSEGFNGNPDAFFDELGPEDVVLLPAFGAPITWVNKLREQDCIVVDTTCGSVLSVWK
                     RVARYASRGFTSIIHGKYYHEETKATASQTLREGGNGKYLIVRNLKETGFVTDFILGR
                     MSAEELCEKLAHGMSPGFDPEEDLQRVGVANQTTMLMSETMAVGEELRKAMVERYGAE
                     TLPEHFELFDTICSATQDRQDALFELLENELDVVIIVGGYNSSNTNNLAIIAKERVPR
                     SYHIASGDCITGDLIRHKPPGTPLDAREEIEEGGWLPDGPVSIGLTAGASTPNSQIGL
                     AIGRILEARGLPPEIALS"
     gene            complement(7405..7617)
                     /locus_tag="VFJ72_14260"
     CDS             complement(7405..7617)
                     /locus_tag="VFJ72_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480666.1"
                     /translation="MKKIFGRLVLLGALVGGAVALGGYLKKSGPSGEEVVQITFDDNT
                     TQSLPASSMESQEFTDIARKLVGMGV"
     gene            complement(7620..8015)
                     /locus_tag="VFJ72_14265"
     CDS             complement(7620..8015)
                     /locus_tag="VFJ72_14265"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET7480667.1"
                     /translation="MATEGNTMTNEGLERVVGDLRALSEDMRSLAVLSADGSLRSSSH
                     APGVDRERAGAMLAALSNLAERAARENGKSHVSQVRVKTEEGFLLLVALEGGGALAAT
                     TGPEARVGLVLYDMRNARGEVQKALGEGR"
     gene            8153..8227
                     /locus_tag="VFJ72_14270"
     tRNA            8153..8227
                     /locus_tag="VFJ72_14270"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8185..8187,aa:Val,seq:cac)
BASE COUNT         1522 a         2863 c         2701 g         1329 t
ORIGIN      
        1 ggccccggcg tcccgcgaga cctcgccgcc cggctcgcgt ccgagaacgt cttcgtcagc
       61 gtgcgcggcg gtagcgtgcg cgtctctccg cacctgtaca acacggagag cgacgtggac
      121 cggctcttcg aggcgctgga ccgcgcgata tactgagccg gtctcagagc cgccgtcagc
      181 ccacgagagg agtcgcgccg tgcgaatcgg tctgcaggtc ccgcgtttta cctggccgga
      241 tggtacagaa gggttgggaa ggaagctggc ggagatcggc cggaccgccg acagcgccgg
      301 tttcgacagc ctctgggtga tggaccactt cttccagatc gcgatggtgg gagaggccga
      361 ggaaccgatg ctcgaaggct acagcgccct gagctacctg gcgggcgtga ccgaacgggt
      421 gaagctcggc actctggtga cgggcgtgca ttaccgttat ccgggcatcc tggcgaagac
      481 tgctaccacg ctcgacgtgc tctccggcgg gcgggcgtac ctcgggatcg gcgcgggctg
      541 gtacgagcgg gaatcagcgg gcctcggcgt gccgtttcca accacgagcg agcggttcga
      601 gcggctggag gaggcgctcc agatcacccg ccagatgtgg tccgatgagg tggcgaccta
      661 cgagggcaaa cactaccggc tggccgagac gctcaacagc ccgcagcccg tatcgcggcc
      721 gcacccgccg atcatgatcg gcggctccgg tgagaagaag accctccggc tcgtggccct
      781 ctacgccgac gcctgcaacc tcttcgccta cggcgggccg gagacgatcc gccacaagct
      841 cgacgtgctg cgcggacact gcgaggacgt gggccgcgac tacgaagaga tcgagaagac
      901 cgccctgggc acggcgaacc tcgcgccgga cggcatgacg gcggacgacg tgatctccat
      961 gtgccgcgag ctcaacggcg cgggcatcca gcacctcatc ttcaacatgc ccaacgtcca
     1021 cgatatagag ccgctggaac gcttcggcga ggaggtcatc ccggccgtca gggagttcta
     1081 gcggcgagat actcgcgctt gagtatcgag tacatcttca tgtcccggag cttgccctgg
     1141 atcctctctc gctcgcgcag ggtcccctcg caggtcattc cggctttctc caggacgcgg
     1201 gcggaggcgg tgttcccggc aatgcagcgg gcctctatcc tgttgagttc catccgctcg
     1261 aagccgaagc ggattatggc gcgtagggcc tcggccgtca ggccccgtcc ccagtagcgg
     1321 cgggagatga cgtagccgac ctcggcgcgg gcatgctccg gactgcggct cacgaagccg
     1381 cacgtcccga tgaacttctc gtcgcccttg tagacgatgc cccagtcgaa cccctcgccg
     1441 gtggcgtagt tgctgaccgt cagctccagg aaggcgcgcg agtcctccat agtgcggtgg
     1501 gtgggccaga tcacgtaccg cgtcacctcc gggtcggagg catagtcgaa catcgcgtcg
     1561 gcgtcttcga ggcgcatctt gcggaggacg agcctgtcag tctccaaccg cggcaggtcc
     1621 ctcaaaactt ccgcgcccct caccgctcct cgatgctcac ccgctctatg ctgtgccacg
     1681 ggaggaactc gtgtccggta gcgtcgccgc tcgggtcctg cgcgtcgagg agcaggccgg
     1741 tctcgttcca gtcgcagacg tggcccatta ggtcgttgcc gatcctggca gacaccgata
     1801 cgaacttgcc ccgcgccagt acatcaggga tcctgggcgc tccctcgtcc agcccttgcc
     1861 gcctgtttct cgcgtctctg ctctccaaag tcctcctccg taatcccggt gcctttgacc
     1921 caaggttaca ccatcgcgcg cccggcgcgc ccggactcta cctgaaagcc agaggcacgg
     1981 agaccccctg gaagctcccg tcgcgcgcgg agttctcccc cacctgcagc acgccctctt
     2041 caccactcag agagtccggg taactcgcgg tgaagcgata cctgccccag gtcgtcgacc
     2101 agtctgtcgt cctgtaggag ccctcgctca ctgccttgcc cgcgctgttc ttgatgcgcc
     2161 acctgccctg accctcgaac ggccgcgcgt agccctttac acggacctcg ccgggaccgg
     2221 cgctgaagcc ggcgcggggc cgcatgacga ccgcccgctc gcccgtggcc gccggagggt
     2281 acgagctcgc gccgttcacc ggtacgtcca ctactatgcg cgccgggtcg ttcaggtaga
     2341 agaccgtgac gggtccggcg ttgttcttca gcaagaggtc cacgaagagg tggtcgccat
     2401 ccatcgcgcg cacggtgtag tagcgggaga tgctcagccc gagccccttg ccgccggtcg
     2461 ccatcgtcga gtccaccgac ggcagctcta tgcggactac ggtgtcgccg ttgatcctgt
     2521 cccactcgta gtacgggacg aactgcgcgg ggctcttccc catccccagg tccaggaccg
     2581 cccgctcata cgtcccgtgg tccccgaacc gcacgccgag cagcgtatcc gcggatgacg
     2641 ccgctcccgc cgggacggaa cccgccagac agagagccag gaccacagcc gccagaagga
     2701 ccgtcagacg ccgcattaga gcgctcctct ccgggatctc cccattgctc gatcccagaa
     2761 taacatcctg cactctatcg cgcctcagcc ggcgtccggc agcagaccgg gcacttcccg
     2821 ccagtccata aaacgaagga tatccggcct cccttcgagg aagacccggt tccacggctg
     2881 tactttcgcc gccgtccaca gaccggcgtc cgcgacggca gcgagggtgt cgggacggtc
     2941 gtcgatcatg ccgaacgcac ccagctcgcg caggacgcct agtttttctg cgggcgtcac
     3001 cgacaacccc acctccgggc cgaagttctc tttcagccag gaccgcaagg cgggcgcgat
     3061 gtcgccgggg aacgggtgac gggttacgaa gtggacgctt agatcgtgcc gttcctgaaa
     3121 gctccgcagg acttcccgcg cgccgggata gggctcgcgt tccaggatgc gctcgggagc
     3181 gaggaccata tcgaagatcc gggtcgtcgt ctcctcccca taggtatcca gcagatgggt
     3241 ccacgtagag acggatcccg agtccagctc gtcgcccgta acccgcagga ccgcctcgcg
     3301 gaccttcgac tcgacgtccc agatggtcga gtccacgtca actccgagga tacgcttcat
     3361 gggcgccagg agatcaaatc ctccaccggc atcgcctcta ccgacccgaa ttctgggtct
     3421 atgacggcgt ggcttgcccc ggcccgccgc gccacgagcg cgtggcggcg caggccgtcg
     3481 gcgtcgtcta tctcggcgag ccggtccggc aggtcgagat gagcagcccg ctcccggaat
     3541 gccctcgccc gttccggcgc gctgaagagg acggcgacga ggaccatccc ctctccagtc
     3601 tctacgctga cgagcggcgc ctcgccgccg gggtcggaga tgacgtagag cggcagcgga
     3661 atctcgccag ccttctccgc ggcaacccgc gccacgtccc cggcgtgaaa gagcccggcc
     3721 ccttctggcg agacgacgag cacgtagtcc gggccggata gagcatactc ttccacgtcc
     3781 cgccagccgg atagctcgcg cgggtccagc ccggcaagac cggagagtcc ctgttcggcg
     3841 gccccggagg agaactcgcc ggcaagctcc cggtcagcga acagggcggc gacggtagcg
     3901 ggctgacctc cgacttccgc ctcagcgcgc ccgtagcggc cgtccgggtg cgcgagcccg
     3961 aagagcggcg gtcgcggcag gtcttcgagc gtcagctcgc gtccgaactc agccatgctc
     4021 ctcccggctc acgaggtcct tcgctccccc gcgcgggaag agtgcggcga tggccaccgt
     4081 cgcgaccccg aagaccagca gcaccaggaa ggccggggag agagcggcgg ccagggctcc
     4141 ggcaagttgt ttataggcgc tctcggggat gcgggagcgg acggcgggct gcaagagggc
     4201 gttcgggtcg gagagcaggc gggtgaggga tgaatcaccg ccggaggccc gctctacggc
     4261 cgcccggtag cggtcgccca gggaatagtt caggagcgcg cccatcacgg cgacgccgac
     4321 cgacccgccg atggtgcgga agaacacgat agaagaggtg gcgatgccgc gccgccgcca
     4381 ggggacggca ttctggacgg ccaccaggta gctcgtgctg gtgaaaccca ggcccagacc
     4441 gatcacgacg accgcgaaca ccacgtaggc gagcgaagtc ccggcgttca tcgggacgca
     4501 cagcgcggcc ccggcggcga tgaggacggc cccgagcaag agcgtcgtcc ggtagcccgt
     4561 ccgcaagagc atccttccgc cgacgaacga tccgaccggc catcctatcg agagcggcgc
     4621 gacgaccgcc ccggcggtca gggccgtgcc gccgagagag ccctgcacga acagcggcac
     4681 gtatacggag acgccgagca agacgcctcc cgccgccagg ttccccagcg cggccacggc
     4741 aaagagccgc tccgaaaaga ggcccagcgg aacgaccggg tcgtccaccc tgctctccac
     4801 gacaacgaag agcgcgagcg tggcaagccc gccgaaaaag aacgccagtg tggccgcgga
     4861 taaagtggga gcgccgccat ctccgagcag cgcgaagagg acggagatca gaccgccggc
     4921 caggagggcg gtgcccagat agtccatgcc gcgggaccga tgctcgaaag actcgcgcag
     4981 ggtgagcgtc aggagaacgg cggcgataac gccgaacggg acgttcacga agaagaccca
     5041 ccgccaggag atgtagtccg tgatgaggcc gccggcggcg ggacctacga ccgccgagat
     5101 cccccaaacc gccccgaaga gtccctgtat ccgcgcccgc gtctccagct cgaagatgtc
     5161 gccgactatg gtgatcgcca ccggctgcac cgcccccgcc ccgagcccct gcaacgtcct
     5221 gaaggctatg agcccggtca tgctctccgc ggttcccgcc agcacgctgc ccagcaggaa
     5281 gaggaagata ccggccaggt aaaccggctt tcggccgtag gtatccgaga gggtgccgaa
     5341 gatggggccg gttacggtag cggccagcag gtaggcggcg aagacccacg agtacagcga
     5401 gagcccgccg aggtccccga tcacggtcgg catcgccgtc ccgactaccg tcgaatccag
     5461 cgccgccagg aaggtcccta tgagcgtagc cagcgttatg agcgccttgc ggcgtctgct
     5521 cagtcccaat ctactacctg cctgtagaac ggtctataga atgatccttg cgaaaggtgg
     5581 atcttagcac cgcagcggtg aaggacccgt gagcacgccc acgatcagag acaaatatcg
     5641 gataggggat tgttagatca gggacttcgg ccggacgagt tgccgcgcca gcgataccag
     5701 atcttcggag gtgaatggtt ttcgtagcac ccggaccgaa ccgtgcgtgc ggtccgcgat
     5761 ggccttcgcg gcggggtctt tcttcgcggc ggtgaggact atggggatct tctgggtctt
     5821 cggcatcgcc ttgaggcagt gacacgcctc gatcccgccg acgtgcggta tcgagacgtc
     5881 catgatgatc aggtcaaccc gccggccgag tgcctcctgc agggcccgct gaccgtccgt
     5941 ggcgctgacg accttgaacc cggcgccggt cagggcgtcg gtcgaggtct gtaacgcggc
     6001 gaggtcatcg tcggccagca ttatgagggg gcgtcctctt tccacgatgg gatattgtac
     6061 acccgcgcgc gacgttgggt agtcccagga atatcctcgc cgacgccccg tccccgatgc
     6121 gttaaactca gcgcgcaaga cctgtccata tcgtaggtgc gagaggtgag cgtgcagcag
     6181 gaaaaatact tccgccgggg cttcaagctg cggggcaatg tccagcatct cctcgaccgg
     6241 gactaccgct cagaggtggt ggaccgcgtc cgggagaacg gcaacgtgtt taccgccgac
     6301 ggcctgacgc tcaggctcgc cgacgagttc gggttctgct atggggtgga tagggcggtt
     6361 gactacgcct tccagaccag ggagaagttc ccggataaga acatctacat taccggggac
     6421 atgatccaca atccctccat gaacgaccgg ctgcgcgaga tgggcatcca gttcctctca
     6481 gagggcttta acggtaaccc ggatgcgttc ttcgacgagc tcggtcccga ggacgtggtt
     6541 cttctgcccg ccttcggcgc tcccatcacc tgggtgaaca agctgcgcga gcaggactgc
     6601 atcgtcgtgg acaccacgtg cggctcggtc ctctccgtgt ggaagcgcgt ggcgcgctac
     6661 gcgagcaggg gcttcaccag catcattcac ggcaagtact accacgagga gacgaaggcg
     6721 accgcctcgc agacgctccg ggagggcggc aacggcaagt acctgatcgt ccgcaacctc
     6781 aaggagaccg ggttcgtgac ggacttcatc ctcggccgga tgagcgcgga ggaactttgc
     6841 gagaagctcg cccacggcat gagccccggc ttcgacccgg aggaggacct ccagagggtc
     6901 ggggtcgcca accagaccac gatgctgatg agcgagacga tggccgtcgg agaggagctg
     6961 cgcaaggcga tggtcgagcg gtacggggcc gagaccctgc cggagcactt cgagctcttc
     7021 gacaccatct gctcggcgac ccaggaccgg caggacgccc tcttcgagct gctggagaac
     7081 gagctcgacg tcgtgatcat cgtcggcgga tacaactcct cgaacacgaa caacctggcg
     7141 atcatcgcca aggagcgggt cccgaggagc tatcacatag cgagcggaga ctgcattacg
     7201 ggagacctca tccggcacaa gccgcccggc acgccgctgg acgcccgcga agagatcgaa
     7261 gagggtggct ggctccccga tggtcccgtg agcatcggcc tgaccgccgg cgcctcgacc
     7321 cccaactcgc agataggtct ggctatcggg cgcatcctcg aagcgcgcgg gctcccgccg
     7381 gagattgcgc tctcctaggt tctcctagac gcccatcccg acgagcttac gggcaatgtc
     7441 ggtaaactcc tggctctcca tggagctcgc agggagtgac tgggtggtgt tgtcatcgaa
     7501 ggtgatctga accacctcct cgccggaggg gccggatttc ttgaggtagc ctcccagggc
     7561 gacggcgccc ccgacgagcg cgcccaacaa gaccagccgt ccgaagatct tcttcatggc
     7621 tacctcccct ctcctagagc cttctggacc tcgccacgcg cgttccgcat gtcgtagagg
     7681 acgagcccga cccgcgcctc cgggccggtg gtggcggcga gcgccccgcc gccttcgagc
     7741 gccaccagca gcaggaaccc ttcttcggtc ttcactcgca cctgggagac atgagacttg
     7801 ccgttctcgc gagcggcacg ttcggccagg ttggagaggg ccgcgagcat agctcccgca
     7861 cgctcccgat ccacccccgg agcgtgcgac gacgaacgaa gactgccgtc cgccgagagc
     7921 acagccagcg atctcatatc ctcggagaga gcccgaaggt ctcccaccac cctctccaga
     7981 ccctcgttcg tcatggtatt accttccgta gccacgcacg ctcctcccgt cccagagatc
     8041 cctgctcgac cgtcgattat atccgatgcc cgtctcccgc tccctcccgg cgccgagcgg
     8101 cggccagcac cttaacaacg cgcctctccg atggtatatt ttccgtccgc aagggcgatt
     8161 agctcagcgg gagagcgctg ccttcacacg gcagaggtcg ctggttcgaa accagcatcg
     8221 cccaccacga agtacctgca aatggcagga aaagatacgc ccccggcttt cggtccgggg
     8281 gctctttact gcaaccgtac tgcaacgcgc tcgctgcact gctgggaagg gacattgggg
     8341 cacgaaagtg gcgccggctg aaagttgcgt ttggaggatt gatgttcgca agggctgatg
     8401 cgaaccgccg gggtc
//
LOCUS       DASSMB010000248         4420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1474303, whole genome shotgun sequence.
ACCESSION   DASSMB010000248 DASSMB010000000
VERSION     DASSMB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474303"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1376)
                     /locus_tag="VFJ72_14275"
     CDS             complement(243..1376)
                     /locus_tag="VFJ72_14275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7480668.1"
                     /translation="MNRPSRPPLRSLGTKLFASHLLVALVGTLTFLAAVSFIAPALFG
                     RLMSGVMSPEHLMTMGEMMDSVSRAFMQTLLYSLLIAALAAAVTAVAASLFTARRISN
                     PVRHMLEATRRISSGSYGERVPAGENDELGALSESLNTMAAALEATEQRRREFIADVS
                     HELRTPLSTLQGYMEGLMDGVVEPSEETWALLYAEAERMRRLVDDLQQLSSAEAGQLT
                     LRLERIFAGEIVDTATESMLPLFADKGVELERSIPKDLPAVTADRDRSVQVLTNLLNN
                     ALRYTPAKGKVTVEAGKRDDEVLFSVRDTGAGMSAEHLPHVFERFYRVEKSRSREGGG
                     SGVGLAISRALIESMGGSIRAESAGPGKGATFSFTLPMARTRK"
     gene            complement(1373..2056)
                     /locus_tag="VFJ72_14280"
     CDS             complement(1373..2056)
                     /locus_tag="VFJ72_14280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480669.1"
                     /translation="MSVRILIVDDEKNLVNLLRGYLERESFEVHEALDGPSAVEVARR
                     VRPDLIVLDWMLPGLDGTRVLGEVRRFSDAYVIMLTARSEEVDKIVGLSAGADDYLTK
                     PFSPGELVARIRAMLRRPRGGDSAADAPLEFGELTIDSARREVRLRGEEVSLTALEFD
                     LLSALASRPGLVFTRRQLLERVWDETYFGSDHVVDVHVANLRKKIEEDPANPRYVQTV
                     RGVGYRFAR"
     gene            complement(2127..2591)
                     /locus_tag="VFJ72_14285"
     CDS             complement(2127..2591)
                     /locus_tag="VFJ72_14285"
                     /inference="COORDINATES: protein motif:HMM:NF025700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HET7480670.1"
                     /translation="MAGRDDSLNRTALSATLHCLTGCSIGEILGMVRGSAFGWSNLAT
                     IIAAIVLAFFFGYSLTMLPLLRSGMALAAVLPLAFASDTLSITVMEIVDNLVVVVIPG
                     AMDAGLLTFLFWGSLALALAIAFVAAFPVNRWLIARGKGHAVVHQHHGGHQA"
     gene            complement(2683..>4420)
                     /locus_tag="VFJ72_14290"
     CDS             complement(2683..>4420)
                     /locus_tag="VFJ72_14290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HET7480671.1"
                     /translation="TLIALGILVSYGYSVGATFFFEGEVFYDAAAMLTTFSLVGHWLE
                     MRSRFATGRAVEALLRLAPDTARVRRGGQEMEIALDQVKVEDEILVRPGDRVPVDGVV
                     ISGSSYVDEAMITGEPVPVAKNEGDRVTGGTVNQTGAFDFRATAIGADTALSRIVQLV
                     QDAQASKAPAQRLADIAGKYLVFVALSAGLISFLVWYFVGSEGLLFAITAAVSTIVIA
                     CPDALALATPTAITVGVGKSAKEGVLFKNATELETTASVDTVVFDKTGTLTVGRPALT
                     DVIPTGGMAEEELLRLAASADSRSEHPMARAIVEGSEARGIQLDPAESFQAIPGHGVE
                     ANVDGKRVLIGNKALMDREDIPLDELEARAAALANDAKTPMYVAVNGEASGLVAVADT
                     IRESARRAVSGLHGLGVKTVMLSGDNRHTAEAVARQLGIDTVLSEVLPGDKASEVNKL
                     QEAGGRVAMVGDGVNDAPALAQADVGIAIGAGTDVAVETAGVVLLKDDPAAVAIAVAM
                     ARKVRGKIKQNLFWAAIYNLLAIPVAAGALYPSLGILLQPEWAALAMSASTVTVTLNA
                     LALNRIRFTNPS"
BASE COUNT          783 a         1428 c         1434 g          775 t
ORIGIN      
        1 ggatatgccg tggaacaggc cccacaccat gagcagcaag aacgcgaccg gcaggacgac
       61 gagcagggtc acggccagta aggtcaacac gatctccata gcttcaactc ctttccttct
      121 acaagaacaa tgtaaagcgg aaagggtgac ctggcttgat cagattggct cattcgtata
      181 gctaaatgtg atgattggct ggcgcatcgc tacagctttc cgggagggaa gcgtggtccc
      241 ttctacttcc tggttcgtgc catcggcagg gtgaagctga acgtcgcccc cttgcccggt
      301 ccggcgctct ccgctcggat actgccgccc atagattcga tgagcgcgcg ggagatggcg
      361 agccccaccc cggaaccgcc gccctcgcgc gagcgggatt tctccacccg gtagaaccgc
      421 tcgaagacgt gcggcaggtg ctcggcggac atgcccgctc cggtgtcccg cacgctgaag
      481 aggacttcgt cgtcccgctt cccggcctcc accgtgacct tgcctttcgc cggggtgtag
      541 cgcagggcat tgttgagcag gttcgtcaat acctgcacgg aacggtccct gtccgccgtc
      601 actgccggca ggtcttttgg gatggacctc tccagttcga ctcctttgtc ggcgaacagc
      661 gggagcatac tctccgtggc ggtatccacg atctctccgg caaagattcg ctccagacgc
      721 agcgtgagtt gcccggcctc ggcgctggag agttgctgga ggtcgtccac gagccggcgc
      781 atacgttcgg cctcggcgta gaggagggcc caggtctctt ccgagggctc gactacgccg
      841 tccatgagac cctccatgta accctggagc gtcgagagcg gcgtgcgaag ctcgtgggag
      901 acgtcggcga taaactcccg gcggcgctgc tcggtcgcct ccagcgcggc ggccatggtg
      961 ttcaggctct cggagagagc gcccagttca tcgttctctc ccgccggcac gcgctcgccg
     1021 tagctgccgg acgagatcct ccgggtcgcc tccagcatgt gccgcacggg gttcgagatc
     1081 ctgcgcgccg tgaacaggct cgcggcgacg gcggttaccg ccgccgccaa cgccgcgatg
     1141 agcagcgagt agaggagcgt ctgcataaag gcgcgggaga cggagtccat catctctccc
     1201 atcgtcatca ggtgctcggg gctcatgacg ccgctcatca accttccgaa gagagcgggc
     1261 gctataaagg agacggccgc gaggaaggtc agggtcccga cgagggcgac gagcaggtgg
     1321 gaggcgaaga gcttggtgcc gaggcttctg agtggaggac gggacggacg gttcaccgag
     1381 cgaacctgta gcctaccccg cgcacggtct ggacgtagcg ggggttcgcg ggatcttctt
     1441 cgatcttttt gcggaggttc gccacgtgta cgtccaccac gtgatcgcta ccgaagtagg
     1501 tctcgtccca gacgcgctcc agtagctgtc ggcgggtgaa gaccagcccc ggacgggacg
     1561 ccagagccga caggagatcg aactcaagag ccgtcaggct cacctcttcg cctcgcagac
     1621 ggacttcgcg gcgcgcagaa tctatggtga gctcgccgaa ctccagcggc gcgtccgccg
     1681 ctgaatcgcc gcctcgcggg cgacgcagca tcgcccggat gcgggccacc aactcaccgg
     1741 ggctgaacgg tttggtcagg tagtcatccg cgccagccga gagcccgacg atcttgtcca
     1801 cctcctccga acgggcggtg agcatgatga cgtaggcgtc ggagaaacgc cggacctcgc
     1861 ccaggacccg cgtcccgtcc aggcccggca gcatccagtc cagcacgatc agatccggcc
     1921 ggacccggcg agcgacctcg acggcggacg gcccgtcgag agcctcgtgg acctcgaagc
     1981 tctcccgttc gaggtacccg cgaagcaggt tcacgaggtt cttctcgtcg tccactatca
     2041 gtatccgaac gctcatgctt ccattctaca gccggctctg gcggagaaac gagagggcag
     2101 gtcggccccc ctccgtgacg cggcatctac gcttggtggc cgccgtggtg ctggtggacg
     2161 acggcgtgac ccttgccacg ggctatcagc caacggttta ccgggaaggc cgcgacgaac
     2221 gctatcgcga gcgcgagcgc cagactcccc cagaaaagga aggtgagcag cccggcatcc
     2281 atggcgccgg ggataacgac caccacgagg ttatccacta tctccatgac cgtgatcgag
     2341 agcgtatccg acgcgaaggc tagcggcaat accgccgcga gcgccatgcc tgaccgcagg
     2401 agcggcaaca tcgtcagcga ataaccgaag aagaacgcga gcacgatggc ggcgattatg
     2461 gtcgctaagt tgctccaccc gaacgcgcta cctctgacca tgcccagaat ttcgccgatg
     2521 gaacagccgg tgagacagtg cagcgtcgcg ctcaacgccg tgcggttgag agagtcgtcc
     2581 ctgccagcca tgacactcct tccgtaactt tggtgcagaa cggcttttat atacccgtag
     2641 ggggtatatt ttaaattcgt gtggcccctg gtcgctccgg tgtcaggagg ggttggtgaa
     2701 ccggatgcgg ttcagggcga gggcgttcag cgtaaccgtg acggtggagg cgctcatggc
     2761 gagggctgcc cattccggct ggaggaggat gccgaggctg gggtagagcg ctccggccgc
     2821 cacggggata gcgaggaggt tatagatcgc ggcccagaac aggttctgtt tgatcttgcc
     2881 gcgcaccttg cgggccatgg cgacggctat cgctaccgcc gccggatcgt ctttgaggag
     2941 caccaccccg gccgtctcga ccgccacgtc ggtgcccgcg ccgatggcga tgcccacgtc
     3001 ggcctgagcg agcgctggcg cgtcgttgac cccgtctcct accatcgcga cccgtccgcc
     3061 cgcctcttgc agcttgttga cctcggaggc cttatctccc ggcaggacct cggagagtac
     3121 ggtatctatg ccgagctggc gcgcgaccgc ttcagcggta tgccggttgt cgccactgag
     3181 catgaccgtc ttcaccccga gaccatgaag cccagacacc gctcgccggg cgctctcgcg
     3241 gatggtgtcg gccacggcga ccaggccgga ggcttcgccg tttactgcga cgtacatcgg
     3301 ggtcttggcg tcgttggcta acgccgcggc gcgggcttcg agttcgtcca acgggatgtc
     3361 ctcacggtcc atcagggcct tgttcccgat caacacgcgc ttgccatcaa cgttcgcctc
     3421 tacgccgtga ccggggatag cctggaagct ctcggcggga tcgagctgta tcccgcgtgc
     3481 ctcggacccc tcgacgatgg cccgtgccat cgggtgttcg ctgcgggagt cggcggaagc
     3541 ggcgaggcgc aacaattctt cctcggccat gcctccggtg gggatcacgt cggtgagagc
     3601 gggcctgccg acggtgagcg tgccggtctt atcgaaaacg acggtgtcca cgctggcggt
     3661 cgtctccagt tcggtggcgt tcttgaagag aacgccctcc ttcgcgcttt taccgacgcc
     3721 cacggtgatg gcggttggcg tggcgagcgc cagggcgtcc gggcatgcga tcacgatggt
     3781 cgagaccgcc gccgttatcg cgaacagtag cccctcggag ccgacgaagt accagacgag
     3841 aaaagagatc aaacccgcgc tcaacgccac gaataccagg tacttgccgg cgatgtccgc
     3901 cagacgctgg gccggagcct ttgaagcctg ggcgtcctga acgagctgca cgatcctgga
     3961 gagagcggtg tccgcgccga tggcggtagc gcggaagtcg aaggcgccgg tctggttcac
     4021 ggtgccgccc gttacccgat cgccttcgtt cttcgctaca gggacgggct cgccggtgat
     4081 catggcctcg tccacgtagc tggagccgga gatcacgact ccgtccaccg gtacccggtc
     4141 gccgggacgc accaggattt cgtcttcgac ctttacctga tccagggcta tctccatttc
     4201 ctggccgccg cgcctgacgc gagcggtgtc gggagccagc cgcaatagtg cctcaaccgc
     4261 gcgtccggtg gcgaagcgac tgcgcatctc cagccagtgt ccgaccagag agaaagtcgt
     4321 gagcatcgcg gcggcatcgt agaagacctc gccctcgaag aagaaggtcg cgccgaccga
     4381 gtacccgtag ctgaccagga tgcccagcgc gatcagggtc
//
LOCUS       DASSMB010000249         5232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1079895, whole genome shotgun sequence.
ACCESSION   DASSMB010000249 DASSMB010000000
VERSION     DASSMB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079895"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="VFJ72_14295"
     CDS             complement(<1..982)
                     /locus_tag="VFJ72_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7480672.1"
                     /translation="MENTALRIEYKPRTGSEAMCEALLDEGVEYLFGYPGGAIMPFYD
                     TLPDYPLKHILTRHEQAAAHAADGYARSTGRVGVVVATSGPGATNLVTGLATAQMDSV
                     PLVAITGQVGRPAMGKDSFQETNVMGMTLPFTKHSFLVENPDELYPTIRRAFEIARSG
                     RPGPVLVDVPKDVQFAACNDNGYRRLREAPAPVESPGLERAAMLLRNAKRPIILAGHG
                     VTLSGAEMELRLLAERTGIPVVTTLLGISAFPEDHPLYLGWPGMHGQASVNRAIDRSD
                     LLFAIGMRFDDRITGAAHKFAPNAKVIHIDVDPSELGKVIKPTVGIAADAK"
     gene            1644..3176
                     /locus_tag="VFJ72_14300"
     CDS             1644..3176
                     /locus_tag="VFJ72_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480673.1"
                     /translation="MSIVTLAGGMPAGAAPDGVQEKKAEIQAAQERLQEIQTKASVSY
                     ETYNSALLGLNDLDEKIDGSKKDLTQAEERVAEAQKNLEDAAAQVYKSGNVGFIDVLV
                     GSEDFSEFASRLKLWVRLLGEERDRFEVVLEARNDLKADQAKLEEQRDRRVATLKSAV
                     KEKEQAEDAASEAEDYLQSLSGDLRAEIQAAAAQRAADAQAAAEAAVEKAAVEKTGPA
                     PAPEMAQVAQVAPQASQSDLRAERKAAEQRAAERADKRAAADAAAKKAERQAELAAKE
                     AAEKKSAKREAAQKRAEEQRAQAEAARQAAKEAAIEQAAAERAAERAAQRETAKKAAE
                     EQAAAEQAAAEQQAAEQAAADQAAAEQAAAEQTTPEATTPETTAPESTVPEATTPAPE
                     PAPSTGGGSCGDTFGGVQPYVAEVGCAVRAQFGLETIYGYRAGDPGDHGTGRALDFMV
                     YSDSALGDQVAEYLLANQSEFGISYIIWQQRINFGSGWQPMEDRGSITANHYDHVHAS
                     FY"
     gene            complement(3250..4182)
                     /gene="thrB"
                     /locus_tag="VFJ72_14305"
     CDS             complement(3250..4182)
                     /gene="thrB"
                     /locus_tag="VFJ72_14305"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET7480674.1"
                     /translation="MISVRVPATSANLGPGYDAVGLALSLSMRISLDRSPTPIVEVHG
                     EGADLIPHGPEHPAYRAARFVAEMVDESDAQFHLIQENSIPPARGLGGSAAALVGGAV
                     AANDLLGRALAPPDLLNLVCDLDGHPDNAAPALLGGLVIGTLTPTGVSAVRLEPKNLK
                     AVVAVPDFEVSTTEARRALPEQVPHRDAVFNVGRSGLLLGALATGEYDLLRVAMQDRL
                     HQPYRSHLIPGLEDVIEAAISNGAYGACLSGSGPTVLAFAPEAPARKIAAAMRSAFEA
                     RGVRAKAWALDVDLRGARVEPPEEVPAPSVVPVI"
     gene            complement(4179..>5232)
                     /gene="thrC"
                     /locus_tag="VFJ72_14310"
     CDS             complement(4179..>5232)
                     /gene="thrC"
                     /locus_tag="VFJ72_14310"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7480675.1"
                     /translation="REWLPEIPDDAIATLGEGETPLIEATKLSERVGARVYLKFEGMN
                     PTGSFKDRGMTVALSRAVAAGSRACICASTGNTAAAAAAYSARAGIKCFVMVPAGKIA
                     LGKAVQVLAHGAEIIQIDGNFDAALKLSIQAAEEMGNVALVNSVNPDRIEGQKTAAFE
                     VCEVLGRPPDALAIPVGNAGNITAYWKGFSEWRAAGYSDESPRMLGFQAEGASPLVAG
                     HDFENPETVASAIRIGAPASKEGALTAARESGGAIESVTDEEILAAQTLLAREEGVFC
                     EPASAAGIAGLLKLAREGRAPKGTVVSVLTGHGLKDPDVILSRVELPEPVEATFEAIA
                     GRMELATAQSEASAKP"
BASE COUNT          937 a         1634 c         1792 g          869 t
ORIGIN      
        1 ccttcgcgtc cgccgctata ccgacggtcg gcttgatgac cttgccaagc tccgacgggt
       61 ccacgtctat gtggatcacc ttcgcgttcg gggcgaactt gtgggcggcg ccggtgatcc
      121 tgtcgtcgaa tcgcattccg atcgcgaaca ggaggtccga gcggtctatg gcccggttga
      181 cgctcgcctg cccgtgcatc cccggccagc ccaggtacag cgggtgatcc tccgggaacg
      241 cgctgattcc gagcagcgtt gtgacgactg ggatgccggt gcgctccgcc aggaggcgca
      301 gttccatctc cgcgccggag agcgtaacgc cgtgtccggc caggatgatc gggcgcttcg
      361 cgttccttag aagcatcgcc gcccgctcca ggcccggtga ttcgaccggg gccggagcct
      421 cgcgcagcct gcggtagccg ttgtcgttgc aggcggcgaa ctgcacgtcc ttcggcacgt
      481 ccacgaggac cggaccgggc cttccgctgc gcgcgatctc gaacgcccgc cggatcgtcg
      541 gataaagctc atcggggttc tcgaccagga acgagtgctt ggtgaagggc agcgtcatgc
      601 ccatcacgtt cgtctcctgg aacgagtcct tgcccatcgc cggacgaccg acctggcccg
      661 tgatcgccac gagcggcacc gagtccatct gcgccgtagc gagtccggtt accaggttcg
      721 tcgcccccgg cccgctggtc gccacgacca cgccgacgcg gcccgtagac ctcgcgtatc
      781 cgtcggcggc gtgcgcggcg gcctgctcgt ggcgggtcag gatgtgtttc agcgggtagt
      841 cggggagggt gtcgtagaac ggcatgatcg cgcctcccgg atacccgaag aggtactcga
      901 ccccttcgtc cagtagcgcc tcgcacatcg cctcgctgcc cgtgcgcggt ttgtattcga
      961 tcctgagagc ggtattttcc acggtttacc tcgttctagt tagtagagaa gacttgtgta
     1021 ccgaccttgc cgaagagcgg ggaatatccc caggcttgcg gctctaggcg gaccggatga
     1081 cgctgtcccg gcccgcccgg ctaattagtc gccgtagggg caggggtaca gctaccgtgg
     1141 atactcgtgg gtgaatgggt ggagtgagct ggtgcgcagc cgcgagcttg agaccggaga
     1201 tctcgatctg ctctccatgt gaaactgtgc tcaaggactt tccttactca ctcgactaca
     1261 agtgcgtgaa gggtatgaca ctttgcaacg gtttgtcaag gcaagaaggg cttcttgccg
     1321 gatcctctac taaaatatca acccgcatgt gatcctccgt caaacgcctg tccttcgtta
     1381 gacgttacgt agattaaaat ttattaaaat ttcgtttcgt gtaagaaaca cattttgtcc
     1441 ggttttaagc catatatagt ggttgtgatt gccaatcatg caacatatag ggtattgcgc
     1501 gtgattagga tggttcctaa agtgtagcgg atttttagga ggggggattc tcctaagcag
     1561 attcccctac gggacaaacg atagttagaa ggagccgggg ccatatgtct cgaagggtaa
     1621 ggtttgcttt tatatttggt gtcttgagta tcgtgaccct tgccgggggc atgccggcgg
     1681 gggccgctcc ggatggagtg caggagaaga aggccgagat ccaggcggcc caggagcggc
     1741 tgcaagagat ccagacaaaa gccagcgtct cctatgagac ttacaacagc gccctgctgg
     1801 gactgaacga tctggacgag aagatagacg gttccaagaa ggacctcacg caggccgaag
     1861 aacgggtggc ggaggcgcag aagaacctgg aagacgccgc cgcccaggtc tacaagagcg
     1921 gcaacgtggg ctttatagac gtgctcgtcg gctcggaaga cttctcggag ttcgcctctc
     1981 ggctgaagct gtgggtgcgg ttgctcggtg aggaacgaga ccgtttcgag gtcgtgctgg
     2041 aggccaggaa cgacctgaag gcggatcagg caaagctcga ggagcagcgc gatagacggg
     2101 tcgccacgct caagtcggcc gtaaaggaga aagagcaggc cgaagacgcc gcctccgagg
     2161 cggaggacta ccttcagtcg ttgagtggcg atctgcgggc cgagatccag gcggcggccg
     2221 cgcagcgggc ggcggacgcc caggcggctg cggaggccgc ggtcgagaag gccgcagtcg
     2281 agaagaccgg gccggctccc gcccctgaga tggcgcaggt ggcgcaggtc gctccgcaag
     2341 cgtcacagtc cgacctgcgg gccgagcgca aggccgcaga gcagcgagcc gccgaacgcg
     2401 ccgacaaacg cgccgcggca gacgccgccg cgaagaaggc cgagcgtcag gcggagcttg
     2461 ccgcaaagga ggccgcggag aagaagtcgg cgaagcggga agccgctcag aaacgcgccg
     2521 aagagcaacg cgcccaggcc gaagcagccc gccaggcggc aaaagaggca gccatcgaac
     2581 aggcagccgc cgagcgcgca gcagagaggg cggctcagcg ggaaaccgcg aagaaggccg
     2641 ccgaagagca agcagcagca gagcaagcag cagcagagca acaagccgcc gagcaagcag
     2701 cggcggatca ggcagcggcc gaacaagctg cggcagagca gacaaccccg gaggctacaa
     2761 ccccggagac gaccgctcca gagagcacgg ttccggaggc cactacaccg gcgccagaac
     2821 cggcgccgag cacggggggc gggtcttgcg gagatacgtt cggtggtgtg cagccgtacg
     2881 tggccgaggt gggctgcgcg gtgcgggctc agttcgggct ggagactatc tatggataca
     2941 gggcggggga cccgggagat cacggcacgg gaagggcgct ggacttcatg gtctattccg
     3001 actcggcgct tggggatcag gtagcagagt acctgctcgc caaccagagc gagtttggga
     3061 tcagctacat catctggcag cagcggatca acttcggttc cggctggcag ccgatggaag
     3121 accgaggttc gatcaccgcg aaccactacg atcacgtcca cgcctcgttc tactaagagg
     3181 cctgtagaga gccgcgcttt atcgggagag cctgtcacag gctctcccgt tttcttcatt
     3241 cgattgaaac tagatcaccg gcacgacgct tggggctgga acctcctccg gcggttcgac
     3301 ccgcgcccct ctgaggtcca cgtcgagcgc ccaggcttta gctcgcacgc ctcgcgcctc
     3361 gaaggcggag cgcatggcgg cggcgatctt tcgggccgga gcttccggcg cgaaggccag
     3421 cactgtcggc ccggatccgg agaggcacgc gccgtacgct ccgttggaga tggccgcctc
     3481 tattacgtct tcgaggccgg ggatgaggtg cgagcggtag ggttggtgca gccggtcctg
     3541 catggcgacg cgcagcaggt cgtattcgcc ggtcgccagg gcgccgagca gcagcccgga
     3601 gcggcccacg ttaaacacgg cgtcccggtg ggggacctgc tccggtagcg cgcgcctcgc
     3661 ctccgtcgtc gagacctcga agtccgggac ggcgacgacg gccttgaggt tcttcggctc
     3721 caggcgcacg gcgctcacgc cggtcggcgt gagggtgccg atgacgagac ctccgagcag
     3781 agccggggcg gcgttgtccg ggtggccgtc gaggtcgcag acgaggttca gcaggtcggg
     3841 cggagccaga gcgcggccga ggaggtcgtt ggcggcgaca gccccgccga cgagggccgc
     3901 cgcgctaccg ccgaggccgc gagctggggg gatggaattt tcctggatca ggtgaaactg
     3961 cgcgtcggac tcgtccacca tctccgccac gaaccgggcg gcccggtagg ccgggtgttc
     4021 gggaccgtgg gggatcaggt ccgccccctc gccgtgtacc tctactatcg gcgtgggcga
     4081 gcggtcgagg ctgatcctca tcgagagcga gagcgccagc ccgaccgcgt cgtagcccgg
     4141 tcccaggttc gccgaggtcg ccgggacgcg gacggagatc acggcttcgc cgaagcttcc
     4201 gactgggcgg tagccagctc catccgcccg gctatcgcct cgaacgtcgc ctccacgggc
     4261 tctggcaact cgacccgcga gaggatgacg tccgggtcct tgagaccgtg ccctgtaagc
     4321 acgctcacga ccgtcccctt cggcgcccgg ccctcgcggg cgagcttgag cagaccggcg
     4381 atccccgccg ccgaggccgg ttcgcagaag acgccttcct cccgagccag tagagtctgg
     4441 gcggcgagga tctcctcatc cgtcacgctc tctatcgcgc cgccagactc gcgcgcggcg
     4501 gtcagagcgc cttccttgct cgccggagcg ccgatgcgga tggcgctcgc gaccgtctcc
     4561 gggttctcga agtcgtgtcc ggcgacgagt ggcgaggctc cctcggcctg gaatccgagc
     4621 atccggggcg actcgtctga gtagcccgcc gcccgccact ccgaaaagcc cttccagtag
     4681 gcggtgatgt tgcccgcgtt gccgacgggg atggcgaggg cgtccggcgg gcggcccagc
     4741 acctcgcaga cctcgaaggc ggccgtcttc tggccctcga tgcggtccgg gttaacggag
     4801 tttacgaggg cgacgttgcc catctcttcg gcggcctgga tcgagagctt cagggccgcg
     4861 tcgaagttgc cgtctatctg gatgatctcc gcgccgtgcg ccagcacctg gacggccttg
     4921 ccgagcgcga tcttacctgc cgggaccatt acgaagcact tgatgccggc ccgagcggag
     4981 taggcggcgg cagccgccgc cgtattgccc gtggatgcac agatacaggc ccggctgcca
     5041 gcagcgaccg cccgagagag ggcgaccgtc atcccccgat ccttgaacga gcccgtcggg
     5101 ttcatcccct cgaacttcag gtagacgcgg gcgccgacgc gctcggagag cttcgtggct
     5161 tctatgaggg gcgtctcgcc ctcgccgagc gtcgcgatgg catcgtccgg gatctccggc
     5221 agccattcac ga
//
LOCUS       DASSMB010000250         7826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1477309, whole genome shotgun sequence.
ACCESSION   DASSMB010000250 DASSMB010000000
VERSION     DASSMB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477309"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..802
                     /locus_tag="VFJ72_14315"
     CDS             275..802
                     /locus_tag="VFJ72_14315"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7480676.1"
                     /translation="MTVEREMLALRDGTVVPVREVRAEDAPALRRLVGRSSERSVYLR
                     YFGPMKELSKEQARHFAEVDGRNRFALVALDPEDEDEIVAVVRYDREGESDTAEYAAL
                     VEDRWQGRGVGIGLTRRVIEAARQNGLRRLCALVLHENTGMLQLLQSLELPEKQRFED
                     GVEHIDIYLRPDQVA"
     gene            complement(1106..2314)
                     /locus_tag="VFJ72_14320"
     CDS             complement(1106..2314)
                     /locus_tag="VFJ72_14320"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7480677.1"
                     /translation="MSFGNGNGTEIVISTPLRTAIGTFGGSLKDVPATDLGATVGKEV
                     ISRSGIEAEQVDQCIIGNILSAGQGMNPGRQVGMKSGLPVETPGMTLNRMCGSGLQAV
                     ISAAQEIALGDADVVMAGGIENMDRAPFLLDKARYGYRMGMPKADMYDHMVYDGLWDV
                     FNDYHMGVTAENVAENYGITREESDEYAVRSHQRAAKAHEEELFDGQIVPVEIKQKKE
                     TVQFTKDEHIREGASVEGLSKLRPVFKRDGGTVTAANASGINDGAAMMLVTNSRKAEE
                     LGLPVAGRLISAAVAGVDPSVMGTGMIPASRLAMKKAGLTVDDLDAVEANEAFSSIAI
                     AVGRELEVPEEKLNPVGGAVALGHPIGATGAVLTVKILHHLERTGGRYGLVTLCIGGG
                     MGIAAIFERI"
     gene            complement(2439..2780)
                     /locus_tag="VFJ72_14325"
     CDS             complement(2439..2780)
                     /locus_tag="VFJ72_14325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480678.1"
                     /translation="MDQKQQKQVNEAAEKFANAIKESYQAVAERGVSAQELNAQLTQD
                     FFNGVINNLRTQADKNRDLAQDLIAQQQKQREASQALAQEGANAYMDFLNSMFSYYQG
                     NLEQAKKSAKQ"
     gene            complement(2954..3286)
                     /locus_tag="VFJ72_14330"
     CDS             complement(2954..3286)
                     /locus_tag="VFJ72_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480679.1"
                     /translation="MANRDKANKAAERLAETTRDSYLTVVDHTVGLQERNVRFAQGLV
                     DSSIKELRYQAESNRAMTQELVERAEKQRDAFQTLVEESVDAYMDFVYAPFSYYKEGL
                     QVAKKATR"
     gene            complement(3352..3768)
                     /gene="phaQ"
                     /locus_tag="VFJ72_14335"
     CDS             complement(3352..3768)
                     /gene="phaQ"
                     /locus_tag="VFJ72_14335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-hydroxybutyrate-responsive repressor"
                     /protein_id="HET7480680.1"
                     /translation="MSKDERKPESALRGPEGRVAEARPRNWLVPVILLSLKDWSSYGY
                     ELMERASTFGFEAMNPGTLYRTLRQMEKEGIVESTWETSKGGPARRMYSITDAGKAYL
                     DFWAKSLEQYQQTMDTFFRLYTGQPLRPEKKGEEKK"
     gene            4027..4764
                     /locus_tag="VFJ72_14340"
     CDS             4027..4764
                     /locus_tag="VFJ72_14340"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET7480681.1"
                     /translation="MSSLEGAVAVVTGAGRGLGRAIAEELGRGGAKIVVNYANSKGPA
                     EEVVQGLGEDNAVAIQADVSDAGQAASLIEQSIEKFGQVDVLVNNAGITIDKTMKKLS
                     PEDWDKVVQTNLNSCYYTVKAALAGFTEQKSGKIINISSFVGQAGNFGQTNYAAAKSG
                     IIGFTKASALELARYNVCVNAICPGFMETDMFSAVSEDVREKIKARIPLGRIGDPIEV
                     ARAVRYLVEDGDYITGQTLNINGGIYM"
     gene            5042..5812
                     /locus_tag="VFJ72_14345"
     CDS             5042..5812
                     /locus_tag="VFJ72_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HET7480682.1"
                     /translation="MGRLSGRVAVVTGAARGIGAAEAIRLAQDGANVAVLDLSAESCQ
                     ETVEAIEAHGAEAIAVACDVSSEQQVEKAFEEIVNRFGRVDILVNNAGLLRDNLSFKM
                     SEDDWDKVLDVHLKGSFLCSRAAQKYMVEQEYGKIVMTSSIVALGNKGQANYSAAKAG
                     LQALARTLALELGRFNVNVNAVAPGWIETEMTKEAAERVGMTMEDMKERFAKNIPLKR
                     FGRVDDIANVVAFLVSDDASYISGETIYVAGGPSSSVI"
     gene            5961..6734
                     /locus_tag="VFJ72_14350"
     CDS             5961..6734
                     /locus_tag="VFJ72_14350"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7480683.1"
                     /translation="MGKRKRYEPGTFSWADLSTSDADSAKTFYGGLFGWEFEDNEIPG
                     GGIYTMCQVEGDTVAAIVQQNEQPGHWNNYVTVTSADETAAKAKQLGANVFEEPFDVM
                     EAGRMAVLADPSGAMLCVWEPREHIGAGRVNDPGCMGWNELQTRDPETAAAFYSALFD
                     WTTEPIEQDGATVYMTIKNQADWMNGGIMPVTEQQGDAPPFWMTYFIVTSCDGAVARV
                     KELGGEVLAGPLEPGAGRIAVASDPQGAVFAVFEGETDE"
     gene            complement(6760..7530)
                     /locus_tag="VFJ72_14355"
     CDS             complement(6760..7530)
                     /locus_tag="VFJ72_14355"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7480684.1"
                     /translation="MSPLAYLEAGGGEQPVLLIHGNFAGKSWWREVLAGPVPNARLLA
                     PDLPGFGESTGVRGFSPSMSRYAESLVRFLDGMGVRRPVLVGHSFGAAVAVELALTSP
                     ERFPAMLLLSPTPLDGLYTPEYLYPVLQSYRFDWHGLRQSLQYVMSPCNPSYLDDLVD
                     EARKMHPANFTGNARILAEWRVNGKARLYGGPVLVASGQRDNLISPYSAQATARAFHA
                     GKYVSLGNVGHSPQIEAPEKVLSLLDVLLDLTEGDASL"
     gene            complement(7565..>7826)
                     /locus_tag="VFJ72_14360"
     CDS             complement(7565..>7826)
                     /locus_tag="VFJ72_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7480685.1"
                     /translation="GERGSIIPLALMKRCHAVVGFYLPQIMSRPDLFVPSLRETLGWI
                     SSGDLKLTIGGSYPLEKAAEAHADLEGRKTTGKLLLHPGGGE"
BASE COUNT         1515 a         2334 c         2430 g         1547 t
ORIGIN      
        1 ggtgatgctt tgacgatact ggagggcgca ctccgatcta ccctcgccga aggagctaag
       61 agtgctgctt gagcaggtgt tctcggagcg ggaactctac ctctatgtcc actaataaca
      121 atccaccggt aattgcggcg agcgtaaagt ctcacgaaag tgaggtctat cacggcctcg
      181 tttgtcatca tccctcaagg gcattagaat accgctatat gtattttgca catagacaag
      241 cagtctccga gagatttgag aggccctggt tccggtgacg gtagagcgag agatgctggc
      301 gctccgagat ggcacggtcg tgccggtacg ggaggtccgg gcggaggatg ctccggctct
      361 caggcggctg gtcgggcgct cgagtgagcg ttccgtgtat ctgcgctact tcgggccgat
      421 gaaggagttg tccaaggagc aggcccggca cttcgccgag gttgacggcc ggaaccgttt
      481 cgcgctggtg gccctggacc cggaggacga ggatgagata gtcgccgtcg tgcgctacga
      541 ccgcgagggt gagagtgaca ccgccgagta tgcggccctc gtggaggacc gctggcaggg
      601 acggggtgtt gggatcggcc tgacccgcag ggtcatagaa gctgcccgac agaacggtct
      661 gcggcgcctc tgcgctctcg tcctgcacga aaacaccggg atgctccagt tgcttcagag
      721 cctggaactg ccggaaaaac agcgctttga ggacggcgtc gagcatatag acatctacct
      781 tcgaccggat caggtcgcct gagcgtggcc gctttcctcg ctgccgcgat aacgcagcgt
      841 ggaaggtacg atcccagaga gtacgaggtt caggtcaaga ccggcgagct ggacctcgac
      901 tccatcaacg cttcttctcc aaggacttcc tcgacgagac cgaaggcatg ctacgggtcg
      961 aggtcgccta caagggcaaa gtcgtcaaac gatccgagac cagtgcccag gccggcgccg
     1021 tgaccgtcaa ctggtcgctc tcacagcagt agacgcaaaa ggcggccccg aagggccgcc
     1081 cacgttccgc aagctgactg ccggcttaga tgcgctcgaa gatggccgca atacccatgc
     1141 cgccaccaat gcagagcgtt accagaccgt agcggccgcc cgtccgctcc aggtggtgca
     1201 ggatcttgac cgtaagcacc gcgcccgtgg cgccgattgg gtgtcccagg gccaccgcgc
     1261 cgcccaccgg gttgagcttc tcctccggca cctccagctc gcgccctact gctatcgcta
     1321 tggacgagaa agcctcgttg gcctccacgg cgtccaggtc atccaccgtg agcccggctt
     1381 tcttcatcgc cagccgggac gcggggatca taccggtccc catcaccgac gggtccactc
     1441 ctgccactgc ggcggagatg agcctgcccg cgactggaag ccccagttcc tcggccttgc
     1501 gggagttggt cacgagcatc atcgccgcgc catcgttgat gccgctggca ttggcggccg
     1561 taacagtgcc gccgtccctc ttgaagaccg gcctgagctt gctgaggccc tcgacactcg
     1621 cgccctcgcg gatgtgctcg tccttcgtga actgcacggt ctctttcttc tgcttgatct
     1681 cgacgggtac gatctggccg tcgaaaagct cctcctcgtg agccttggcc gcccgctgat
     1741 ggctgcgcac ggcatactcg tcggactcct cgcgggttat gccgtagttc tccgcgacgt
     1801 tctcggccgt cacgcccatg tggtaatcgt tgaagacgtc ccacaggcca tcgtagacca
     1861 tgtggtcgta catatccgcc ttcggcatac ccatccggta accgtacctc gccttatcca
     1921 gaaggaacgg cgcccggtcc atattctcga tgccgccggc catcaccacg tcggcgtcgc
     1981 caagggctat ctcctgggcg gcggagatta cggcctgaag acccgagccg cacatccggt
     2041 tcagcgtcat gccgggcgtc tcgaccggaa gtccgctctt catgccgacc tgacggccgg
     2101 ggttcatacc ctgtccggcc gagaggatgt tgccgattat gcactgatcg acctgctcgg
     2161 cctcgatgcc ggagcgggag atgacctcct tgccaaccgt cgccccgagg tccgtcgccg
     2221 gaacatcctt gagagaaccg ccgaacgtgc ctatagccgt cctgagtggc gtagagatca
     2281 cgatctccgt gccgtttcca ttgccaaaac tcatgctgct cctttcccgc ttgatcttgt
     2341 aaaagaatac accaaaaacg agagggccgg accaaatggc ccgaccctct aaaaagacct
     2401 gttcaggcct taactctctg ctacgtagtt gttactgctt actgcttggc gctcttcttg
     2461 gcctgctcca ggtttccctg gtagtaggag aacatggagt tcaggaaatc catgtaggcg
     2521 ttcgcgcctt cctgggcgag cgcctgagaa gcctcgcgct gcttctgctg ctgcgcgatc
     2581 agatcctgcg ccaggtcccg gttcttgtcc gcctgcgtcc tcaggttgtt gatgacgccg
     2641 ttgaagaagt cctgcgtgag ctgcgcgttt agttcctgag ccgaaacacc ccgctccgcg
     2701 accgcctggt aagactcctt tatcgcgttg gcgaacttct ccgccgcctc gtttacctgc
     2761 ttctgctgct tctgatccat gatctactcc tctctgtctt tcaacttcca actagtctct
     2821 atacccacct ggaagcctct ctacacttct acctcaagcc tttgctattg cgcttatccg
     2881 tgtgacccct ggagtaagca aaaggagcga gccgcaaagc ccgctccttt gtacctccaa
     2941 aacttgcggg agcctaccgg gtagccttct tggcgacctg gagaccctcc ttgtagtagc
     3001 tgaagggcgc gtagacgaag tccatgtaag cgtccaccga ctcctccact agggtctgga
     3061 aagcgtcgcg ctgcttctcg gcgcgctcga cgagctcctg ggtcatggcc cggttgctct
     3121 cggcctggta gcgaagctcc ttgatcgagc tgtccaccag tccctgcgcg aagcgaacat
     3181 tccgctcctg cagcccaacg gtgtgatcca caaccgtcag ataggagtcc cgcgtggtct
     3241 ccgcgagccg ctccgccgcc ttgttagcct tgtctctgtt agccattagt gccaactcct
     3301 tcctcgcggt actcccgcga aattgcttta ccgccgcctg ctttaccgtt tctacttctt
     3361 ctcttcgcct ttcttctccg gcctcagcgg ctggccggtg tagagccgga agaaagtgtc
     3421 catggtctgc tggtattgct ccagcgactt ggcccagaag tcgaggtaag ccttccccgc
     3481 gtccgtgatg gaatacatcc ggcgcgccgg accacccttg gatgtctccc aggtggactc
     3541 gacgatgcct tctttctcca tctgccgcaa cgtccggtac agcgtcccag ggttcatggc
     3601 ctcgaaccca aatgtcgacg cccgctccat gagctcatac ccgtaggaac tccagtcctt
     3661 gagcgacaac aggatcaccg gcaccagcca gttcctcggc ctcgcttcgg ctacccgacc
     3721 ctcagggccg cggagcgccg actccggctt ccgttcgtct ttactcatca ctctctcttc
     3781 catactctat gtgcatttta catatatata ctgcaacgtc aaggatattg acgaaattat
     3841 tattttgaca ttgtaactct ttgcgcctca aatcttaagg gtcatctaga tgaccagagc
     3901 acatatttcg gtacgagatc gcccatagcc gtctatatat gcctattgta tatagtttgc
     3961 tccgaatttt acctatcgct agacttgggt agaggagagg accaaaccag gggaaaggag
     4021 aatttgatga gtagtctaga aggagctgtc gctgtcgtaa cgggggccgg gagaggtctc
     4081 gggcgcgcca tcgccgagga gttagggcgg ggtggggcga agatcgtagt caactacgcc
     4141 aacagcaagg ggcctgcgga ggaagtggtt cagggtctcg gcgaagacaa tgcggttgct
     4201 atacaggccg atgtctccga cgcgggacag gcggcgagtt tgatcgagca gtccatcgag
     4261 aagttcggcc aggtagacgt gctggtgaac aacgccggca tcaccataga caagacgatg
     4321 aagaagctct cgcccgagga ctgggacaag gtcgtgcaga cgaacctgaa cagttgctac
     4381 tacactgtca aggcggcgtt ggccgggttc accgagcaga agtccggcaa gatcatcaac
     4441 ataagctcgt tcgtcgggca ggcgggtaac ttcggccaga ccaactacgc ggcggcgaag
     4501 tccgggatca tcggcttcac gaaggcgtcg gcgctggagc tggcccgcta caacgtgtgc
     4561 gtgaacgcca tctgccccgg ctttatggag acggacatgt tcagcgcggt ctccgaggac
     4621 gtgcgggaga agataaaggc ccgcataccg ctcgggcgca ttggtgaccc catagaggtc
     4681 gcccgcgccg tccgctacct ggtcgaggac ggcgactaca tcaccggcca gacactcaac
     4741 atcaacggcg gtatctacat gtaacccgcc ccgaacagcc cagagagccc ccttcccaaa
     4801 ttctggagag ggggctatat cttcgccatg agtggcgcaa ggcgcgtcag ccaaaacatc
     4861 tgctcacctc gccaatacgc tatactgcgt gcctgatcta cttcgtaagc taacctcccg
     4921 ctttctgcac atatctacgc tagacactta tattcgattc gtatacaatt aacgaatatg
     4981 caacgtattc cacatcttct ggcgtggaat ggtaggtatg tctagcaagg aaggtggatt
     5041 cgtgggaaga ctcagtgggc gtgtagcggt cgtaacgggt gctgcccggg gaatcggggc
     5101 ggcggaggcg attcggctgg ctcaggacgg tgccaatgtg gcggtcctgg atctttccgc
     5161 ggagtcctgt caggagacgg tcgaggccat cgaggcgcac ggggcggagg ctatcgccgt
     5221 ggcgtgtgac gtgtcgagcg agcagcaggt ggagaaagcg ttcgaggaga tcgtgaacag
     5281 gttcggccgg gtggacatcc tggtgaacaa cgcggggctt ctcagggaca acctgtcgtt
     5341 caagatgagc gaggacgact gggataaggt cctggacgtg catctcaagg gcagtttcct
     5401 ttgttcgcgg gcggcccaga agtacatggt cgagcaggag tacggcaaga tcgtgatgac
     5461 ctcctctatc gtggcgctcg ggaacaaggg gcaggccaac tactcggcgg ctaaggcggg
     5521 gcttcaggct ctggcgcgga cactggcgct ggagctcggg cggttcaacg tcaacgtcaa
     5581 tgcggttgcg ccgggttgga tcgagacgga gatgaccaag gaggctgccg agcgcgtcgg
     5641 catgacgatg gaggacatga aggagcggtt cgccaagaac atcccgctca agcgcttcgg
     5701 gcgggtggat gacatcgcca acgtcgtggc gttcctggtc tccgatgacg cttcgtacat
     5761 cagcggcgag acgatctacg tcgcgggcgg gccttccagc tccgtgatct agggctctcg
     5821 aatatcagga cttttgggcg gggatgcctc cccgcctttt ttgttgcccg cacgctcttg
     5881 atactcgttt tgctacttgg gaatcaccca ttcgcccgta gacttttgct aagagcgaag
     5941 gcgttatcga ggaggcaagg atgggcaaga gaaagcgtta cgagccggga actttcagtt
     6001 gggcagacct gtcgacgagc gacgccgata gcgccaagac cttctacggc gggctgttcg
     6061 gctgggagtt cgaggacaac gagatccccg gcggcggtat ctacacgatg tgccaggttg
     6121 agggcgacac ggtagcagcc atagtccagc agaacgagca gcctggacac tggaacaact
     6181 acgtcaccgt tacgagcgct gacgagaccg ctgcgaaggc gaagcaactg ggcgccaacg
     6241 tgttcgagga gcctttcgat gtgatggaag ccggtcgtat ggcggtactc gccgacccga
     6301 gcggcgccat gctgtgcgtc tgggagccgc gggagcacat cggcgcgggc cgggtcaacg
     6361 accccggctg catgggctgg aacgagctac agacgcgcga ccccgagacg gccgctgcct
     6421 tctactcggc gctcttcgac tggactacgg agcctataga gcaagacggc gcgacggtct
     6481 acatgacaat caagaatcag gccgattgga tgaacggcgg aatcatgccg gtgacggagc
     6541 agcaaggcga tgcgccgccc ttctggatga cctacttcat cgtgacctcg tgcgacgggg
     6601 ccgtggcgcg cgtgaaggag ttgggcggag aggtgctggc cgggccgctg gagccggggg
     6661 cgggcaggat cgctgtcgcg agcgacccgc agggcgcggt gttcgccgtg ttcgagggcg
     6721 agacggacga gtagatccgg ctcagggcga tgcttccctc tagagggaag catcgccctc
     6781 cgtgagatcc aacaggacat cgagtaggga cagcaccttc tccggagcct cgatctgcgg
     6841 gctgtggccg acgttcccga gggagacgta cttaccggca tggaatgcgc gggccgtggc
     6901 ctgggccgag taaggagaga tcaggttatc ccgttggccg gacgctacca ggaccggccc
     6961 gccgtagagc cgggctttgc cgttcaccct ccactcagcg aggatgcggg cattgccggt
     7021 gaagttcgcg ggatgcatct tcctcgcctc atcaacgagg tcgtcgaggt acgaaggatt
     7081 gcatggactc atcacgtact ggagagactg gcgcagcccg tgccagtcga acctgtagct
     7141 ctggagcacc gggtagaggt actccggagt gtagaggccg tccaggggag tggggctgag
     7201 gagaagcatg gctgggaacc gctcgggcga ggtcagggca agctccacgg cgaccgccgc
     7261 tccgaaggag tgaccgacga gcacgggacg ccggacgccc atgccatcga gaaagcgaac
     7321 caggctctcc gcataacggc tcatggaagg ggagaagccg cggactccgg tactctctcc
     7381 gaaacccgga aggtcagggg ccaggaggcg ggcgtttgga accggaccgg ccagcacctc
     7441 gcgccaccag gacttgccgg cgaagttgcc gtgaatgagg aggacgggct gttcgccgcc
     7501 gccagcctcc agatatgcca gcggactcat cggccgggga gcagcgtgtt gttgagggaa
     7561 gtagttactc tccgccgcct gggtgtaata acagcttgcc ggttgtcttg cgtccttcga
     7621 ggtcggcgtg ggcttcggcg gccttttcga gagggtagga acctccgata gtgagcttga
     7681 ggtcgccgga ggagatccaa ccgagagtct cccgcaggct ggggacgaag aggtccgggc
     7741 ggctcatgat ctggggcagg tagaacccga cgaccgcgtg gcagcgcttc attagcgcga
     7801 gggggatgat actaccccgc tccccg
//
LOCUS       DASSMB010000251        13422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1711512, whole genome shotgun sequence.
ACCESSION   DASSMB010000251 DASSMB010000000
VERSION     DASSMB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 13422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13422
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1711512"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /gene="zwf"
                     /locus_tag="VFJ72_14365"
     CDS             complement(<1..1234)
                     /gene="zwf"
                     /locus_tag="VFJ72_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7480686.1"
                     /translation="MADTQVLQNVLRDEQRLPRVPEPGIMVIFGASGDLTARKLVPAL
                     YDLAAQRRLPLEFAVVGVSRTEMSHEEFREKLHKSLKEHRSGKVSDDVWESFSKGIFY
                     LAGDSKKAEAYDELKEFLKKLDSERGTQGNRAFYLSTSPSLVPAIVQRMGEAGMNDGE
                     DGGWSRLVVEKPFGRDLESAQELNEEIRRYFDESQIYRIDHYLGKETVQNILALRFAN
                     GIFEPIWNQHYVDHVQITVAEDIGVGTRGAFYEEAGALRDIVQNHVMQILCLTAMEPP
                     VAFDADSLREEKVKVLKAVRRIPEDDVEHYAVRGQYERGWIWGEEVEGYREGKDVASD
                     SVTETFVALKVYVDNWRWAGVPFYIRAGKRMPKKATEIAIQFRPTPHTPFAHDDTKSL
                     DPNVLVVRVQPEEGISLKI"
     gene            complement(1252..2187)
                     /gene="gnd"
                     /locus_tag="VFJ72_14370"
     CDS             complement(1252..2187)
                     /gene="gnd"
                     /locus_tag="VFJ72_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET7480687.1"
                     /translation="MEIGIYGLGRMGGNMVRRLVRSGEHRVVAGNRSSGPVDEAVSDG
                     AEGAYSMEELVEKLSGDGPRAIWTMVPAGDVTEQALLKFADMMDAGDILIDGGNSYFR
                     DSIRRAEMLKERDIRFLDAGTSGGVWGLQVGYCLMVGGDSDAYEHVEPALKTLAPENG
                     YAYLGDAGAGHFTKMVHNGIEYGMMQAYAEGFEILEKSQYDLDLRAISSLWNQGSVVR
                     SWLLELAESAFEKDANLDSIRGYAEDSGEGRWTVIEAINESVPAATIAGSLFARFASR
                     QDDSFAMKVIAALRGEFGGHAIQETSTKYPEDGKK"
     gene            complement(2255..3352)
                     /gene="tal"
                     /locus_tag="VFJ72_14375"
     CDS             complement(2255..3352)
                     /gene="tal"
                     /locus_tag="VFJ72_14375"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7480688.1"
                     /translation="MNERLRKLQEIGQSVWIDSLSRDDIKEGNLEGMIEDGVVGVTSN
                     PTIFQKAISESSLYDEQLEELSQREEDPKEIFLELAKQDIQDACDLLMPVYEQSGKLD
                     GYVSLEVSPDIAYDTPKTVAEAKRLHEMVDRPNLLVKIPATVPGLAAIEEMIAAGKSI
                     NVTLIFSLSRYRAVAGAYIRGLQRLVASGGDPSSVASVASFFVSRVDTEGDKRLDEVG
                     RDDLKGRLAIENAKLAYAAFEEIFSGDDWEFLESKGATKQRPLWASTSTKNPDYPDTI
                     YVDNLIGPDTVNTMPASTLEATMDHADVKPTLQGGADEARKFLKELREAGVDYDDVVE
                     TLEREGVEKFFDSFEELLEGIKSKGRQLARS"
     gene            complement(3443..5518)
                     /gene="tkt"
                     /locus_tag="VFJ72_14380"
     CDS             complement(3443..5518)
                     /gene="tkt"
                     /locus_tag="VFJ72_14380"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7480689.1"
                     /translation="MDLKSRNIEAVSINTIRALAMDAVEKAASGHPGTPMSMAPVAYT
                     IYTKLMRHNPENPDWKDRDRFILSAGHASMLQYSLLHLTGYDLTIEDLKNFRQWESRT
                     PGHPEHFMTPGIETTTGPLGQGFANGVGMAMAERFLAGRYNRIGHEIVDHHIYAICSD
                     GDLMEGVCQEAASIAGQNALGKLVYVYDDNRITIDGTTALSFDGEDKGKRFEAYGWHV
                     QHVDDSEDLDAIEDALINARDEEERPSLVIVRSHIAYPAPNAIDTAAAHGSALGEDEI
                     RLAKEAMSMDPDEHFAVSDEVYEHMNMAEKGRDLEAEWEDRFSAWSEEFPELAEEWEV
                     AWSGRPMPGYEEKLPRWDPAETEKLATRKAGAAVMDAFQDYTPTMVGGAADLVQSTST
                     TFSNGGMFSSTHTGRNIAWGIREHGMGSAVNGIAVHGGMVRPYGSTFLIFSDYMRPAI
                     RLSALMDLQVAWVYTHDSVGLGEDGPTHQPIEHYMALRAIPNLTLIRPADPNETSMAW
                     RATLEAEGPVALLLTRQNVPVLDPNTAEGALRGAYVLADADGGEPDVILIGTGSEVSV
                     ALEARKALSEKGVDARVVSMPSWEIFDAQDQGYKDSVLPPNIETRVSVEAGITSGWER
                     YVGFKGTSIGINRFGASAPGETVLEKLGIIPEKVANAALELLGSSERVSEESGTPAVE
                     ATSPEEGHS"
     gene            complement(5657..6094)
                     /gene="ssb"
                     /locus_tag="VFJ72_14385"
     CDS             complement(5657..6094)
                     /gene="ssb"
                     /locus_tag="VFJ72_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET7480690.1"
                     /translation="MVSFNRVIIAGNLTRDPELRFTGSGIPVCEFGMAVNRVRSKNEE
                     VDFFDVSAWRELGETIANYKKKGDPILVEGRLQYRTWESQDGSKRSKVDIVADNVQFL
                     GGRSDGDGASGGGGGNRRGAGQRQGGQRDDVDINEEDFDDIPF"
     gene            complement(6162..6905)
                     /locus_tag="VFJ72_14390"
     CDS             complement(6162..6905)
                     /locus_tag="VFJ72_14390"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7480691.1"
                     /translation="MNQRADYGIDAPDIVRNFALAGISSLSAGAFYYAFAGSRPALAA
                     LVVGIGVIWGICGLGTAALMILSSRRGKLKERDRILGSIPWRGDEAVLDAGCGRGLML
                     IGAAKRLIGGRAVGVDIWRKRKQSGNGPEAALANARIEGVEERIEVVEADLQKLPFED
                     DSFDVILSSLVLHNIPEREGRAAALLALARTLKPGGRIAILDIWDTGEYAKMLRESGM
                     REVSRSKLRFDIFPPARLVTARKPGSGEV"
     gene            complement(6910..7878)
                     /locus_tag="VFJ72_14395"
     CDS             complement(6910..7878)
                     /locus_tag="VFJ72_14395"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET7480692.1"
                     /translation="MDRPPHSRGPGIAKTNRRPLGETLRRALRGTLPLLLSCLLLLAS
                     GCAGVRDLASGRSLGGVSPPPSGSLSVSFIDVGQGDGVLVQAGGENYLIDAGRSEAGP
                     EVVDFLRSRGVRDLDGMVATHPDADHIGGLPEVLDAFDVATVYLSGDTDKSTITYNTF
                     LRRVQAEGSRVEEARAGKLMDWGGVDITVIAPPPDAGGGLFEDSNDNSVSLLLTYGTA
                     RILLAGDAEAKEEEYMANGPYTGPVTVLKVDHHGSNTSSTPLFLNAFRPKIAVIQVGE
                     NSYGHPTPETLARLREYGAKVFRNDEDGDIIVTIKDQKVDVAVTKP"
     gene            complement(7842..8546)
                     /locus_tag="VFJ72_14400"
     CDS             complement(7842..8546)
                     /locus_tag="VFJ72_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480693.1"
                     /translation="MRDEFMITRQGKQYVLFAGLLDEAHNRGLKGIDTELIQVPDDSN
                     GNVAVVKATVRMEDDRVFSGIGDASPNNVGRNIAPHLIRMAETRAKARALRDAVNVGA
                     TALEELSDGDDAPPADNFTRTRNQPTPIHGGRQSERREEDPAQSSRAAKEATVNGQAE
                     APAAPAEKPPAGKSRSGTLKARKSQVDLLKTLAVEMRGENGVERLESRLGKPLTDLTR
                     AEADEWIDRLTPEDRE"
     gene            complement(8639..8989)
                     /locus_tag="VFJ72_14405"
     CDS             complement(8639..8989)
                     /locus_tag="VFJ72_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480694.1"
                     /translation="MVRGLILLAVGVVPAFFLVFVTLFGDSSGLGDYALAYGMTFFAY
                     LLVAFVAGFVWDEGRPVYVLWLVAPAVIVIVWYVTQEPQVTVLGIGSLAIALVGTLLG
                     VFVGTRTRSRARSS"
     gene            complement(9065..9931)
                     /locus_tag="VFJ72_14410"
     CDS             complement(9065..9931)
                     /locus_tag="VFJ72_14410"
                     /inference="COORDINATES: protein motif:HMM:NF005992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7480695.1"
                     /translation="MERIDNLLPDAGKAMSGSSARRSKQAEALRLDDQVCPHCGRELM
                     AEWVEFPPTLQKKYEKPGEWYYHPCTPECEQKNEQREWELMRRDSRVATLKERSGLSK
                     RMKGFTFASFDPFANPPSSTRALEKVQTYLKSWEENRETGRGLYFCGDVGTGKTHLAV
                     AVMNELISRRRVPALFVTVPELLDNMRGAYNDPGRDIDEWMAAVKNADLLLLDDLGAE
                     RANEWVRERIFVIVNHRYREELPTIFTSNIGPKDLASQLGERTASRIIGMCDWISLEG
                     EDYREAEVRRGL"
     gene            complement(9861..10646)
                     /locus_tag="VFJ72_14415"
     CDS             complement(9861..10646)
                     /locus_tag="VFJ72_14415"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET7480696.1"
                     /translation="MERVRLTPPGGRGARGERARLYVRWMPHIGAEAVALYELLRILP
                     DIGRDSVEVEEVAELLRSTPESVEAAFETLTETGFIVERDGWMEVAEHPPTAAAKDQK
                     QERTTRIVRETAEEKPVYDIEVIRAEPEGVSADDYFRYMGSLPAPHILEFLNGYCGRD
                     GLSPEVVREALKIASERDARRVGYVRSILERWVERGVKTVEDVERFEQDRRLRLVEQS
                     GAVRGGALKLREGGKGEGVSDGANRQSAARRREGYEWLFGETE"
     gene            complement(10714..11052)
                     /locus_tag="VFJ72_14420"
     CDS             complement(10714..11052)
                     /locus_tag="VFJ72_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480697.1"
                     /translation="MSEDRDRNNTNSEREDQAKLLAGRISCVIGVLFAVYGVVIVLGG
                     GGASVTSGVIGAGLGILGYFLGSRSLGTATIIFSVVVLFFGLAASQGLIPGLDASDRG
                     LPSTELRGKD"
     gene            11108..12520
                     /locus_tag="VFJ72_14425"
     CDS             11108..12520
                     /locus_tag="VFJ72_14425"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET7480698.1"
                     /translation="MRFERKLPGTNEVFQRDGTPRRTYRQVLDELARTGTSRWREKLK
                     QAHGLLLNEQYAFGIKEGDKTHPTDWIPRIVPATDWERLERGLTQRLRAINEFLRRLE
                     AGREEVVPREVVESSILYDTSWQDRFGPVPVRQIAFDVVAVEGPSGGWDYVVIEENAK
                     MPVGLAAMTRRRRMSSEVFFPESHRELPVRSLDGWLPRLGDALRAASPKGADATLAVV
                     SSGPADQFYLDHNIYAAEMDAILTETKDIGVDGDGYLMHGPTGRRIDVIYERVDEDRL
                     YAEVPGLLECHVSGKTHVLFAPNSEIIDDKGVGAFVPEMVRTYLGEEPLIQNARTWSL
                     AVEEDRRYVMDHFEELVVKSRGGYGGKEVMIGPEEPKESIEKFRRQVEENPVEYIAQE
                     MIDFSTHVLCEPDEDDFLLRDSFADYRMLVLSPDPEDPDVVEMVPGSLSRVAAPGKHI
                     VNISSGGKMKDTWVLGEAGQ"
     gene            12517..>13422
                     /locus_tag="VFJ72_14430"
     CDS             12517..>13422
                     /locus_tag="VFJ72_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480699.1"
                     /translation="MNYGEIISSAVRLTWRNKFLWFFGFFAGAGTSFNVPGGGSSGGD
                     GLGGGLPAPLRGITSWVQDNLALFLTVVITLAVLFVVVGIALSIISQGALVESVAALD
                     RGEERRFGSTWRAGTANFWRVLGLKVLFVLISLGLALVMTLVLVLPTVGIFFATDSTA
                     ARVIAVIFAVLAGIALLLLIFLPLAVIAQWALRRLVVDRERIVESVRTGYGLFRRNIG
                     RSLLVWLIQLGLMLGAGIVMLIALIILGLVLVGPAFLLVRAEYTAAAIALGAVGGTIL
                     LAVFFVLSAVLGTFNSSYWTLAYLRL"
BASE COUNT         2239 a         4458 c         4103 g         2622 t
ORIGIN      
        1 cgatcttgag cgagatgccc tcctccggct gcacccggac aacgagcacg ttcgggtcga
       61 ggctcttggt gtcgtcgtgg gcgaagggcg tgtgtggggt cgggcggaac tggatcgcga
      121 tctccgtcgc cttcttcggc atcctcttcc ccgcccggat gtagaacggc acccccgccc
      181 accgccagtt gtccacgtac accttgagcg ccacgaaggt ctcggtcacg gagtcggagg
      241 caacgtcctt cccctcgcga tagccctcga cctcctcgcc ccagatccag ccgcgctcgt
      301 actgcccgcg cacggcgtag tgctccacat cgtcctccgg gatgcgccgg accgctttca
      361 agaccttcac cttctcctcg cgtagcgagt cggcgtcgaa ggccacgggc ggctccatcg
      421 ccgtgaggca caggatctgc atgacgtggt tctggacgat gtctcgcagg gcgcccgcct
      481 cctcgtagaa cgcgccgcgg gtaccgacgc cgatgtcctc ggcgaccgtg atctggacgt
      541 ggtccacgta gtgctggttc cagatcggct cgaagatccc gttggcgaaa cgcaaggcca
      601 ggatattctg gaccgtctcc ttacccaggt agtggtctat ccggtagatc tgagactcgt
      661 cgaagtagcg tcggatctcc tcgttcaact cctgtgcgct ctccagatcc cgcccgaacg
      721 gcttctcgac cacgagccgc gaccagccgc cgtcctcccc atcgttcatc cccgcctctc
      781 ccatccgctg cacgatagcc ggaacgagcg acggcgacgt ggagagatag aaggcccggt
      841 tcccctgcgt cccgcgctcc gaatcgagct tcttcagaaa ctccttcaac tcgtcgtagg
      901 cttcggcctt cttagagtcg ccggcgaggt agaagatgcc cttggagaaa gactcccaca
      961 cgtcatccga caccttgccg gaccggtgct ccttgagtga tttatgtagc ttctcccgga
     1021 actcttcgtg gctcatctcc gtgcgagaga cgccgaccac cgcgaactcc aaaggcagtc
     1081 ggcgctgggc ggccagatcg tagagggcgg gtaccagctt gcgcgccgtc aggtcgccgg
     1141 acgccccgaa gatgaccatg atccctggct ccggcacgcg cggtagccgc tgctcgtccc
     1201 ggagaacatt ttgaaggact tgagtatcgg ccacgtctgg cccctctcta actacttctt
     1261 gccgtcttcg ggatacttcg tgctcgtctc ctgaatggcg tggccgccga actcgccgcg
     1321 cagcgccgcg atgaccttca tggcgaagga gtcgtcctgg cgggacgcga accgggcgaa
     1381 gagcgagccc gcgatggtcg ccgccggtac gctctcgttt attgcctcta ttaccgtcca
     1441 gcgtccctcg ccggagtcct ctgcgtaacc ccggatgctg tcgaggttcg cgtccttctc
     1501 gaaggcgctc tcggccaact cgaggagcca gctacggaca acgctgccct ggttccaaag
     1561 gctggatatc gcgcgcaggt ccaggtcgta ctgggacttc tccaggatct cgaacccctc
     1621 ggcgtaggcc tgcatcatcc cgtactcgat gccgttgtgg accatcttgg tgaagtgtcc
     1681 ggctccggca tccccgagat aggcgtagcc gttctctgga gcaagtgtct ttagcgcggg
     1741 ctcgacatgc tcataggcgt ccgaatctcc gccgaccatc aggcagtaac ctacttgaag
     1801 gccccagacg ccaccgctgg taccggcgtc gaggaagcgg atgtcccgct cctttagcat
     1861 ctcggcccgg cgaatcgagt cgcggaagta ggagttgcca ccgtctatga ggatgtctcc
     1921 cgcgtccatc atgtcggcga acttaagaag ggcctgctcg gtcacgtcgc cggcggggac
     1981 catcgtccag atagcgcgcg gcccgtcacc ggagagcttt tcgaccagct cctccatcga
     2041 ataggcgccc tcggccccgt cggagaccgc ctcgtccacc ggcccggagg aacgattccc
     2101 tgcgaccacc cggtgctccc cgctccggac gagccggcga accatgttgc cgcccatgcg
     2161 gcccagaccg tagatcccta tctccatcgt tcctcctctt ccgaagcggt cggctctact
     2221 tccagttttg ccttcaggtt ttgccttaag gtctctagct gcgggctagc tggcggcctt
     2281 tgctcttaat cccctccagc aactcctcga aggagtcgaa gaacttctcg acaccctctc
     2341 tctccagggt ctccaccacg tcatcgtagt ccactccggc ctcgcgcagc tccttcagga
     2401 atttcctggc ctcgtccgcg ccgccctgca gggtcggttt gacatcggcg tggtccatcg
     2461 tggcttcgag cgtggaggcg ggcatggtgt tgaccgtgtc gggaccgatg aggttgtcca
     2521 cgtagatcgt gtccgggtag tccgggttct tggtcgaggt cgaggcccac aagggacgct
     2581 gcttggtcgc gcctttcgac tcaaggaact cccagtcatc gccggagaag atctcctcaa
     2641 acgccgcata cgcgagcttc gcgttctcta tggcgaggcg gcccttgagg tcgtcgcggc
     2701 ctacttcatc cagtctctta tcaccctcgg tgtccacgcg ggagacgaag aagctggcga
     2761 cgctcgcgac acttgaaggg tctcctccag aggcgaccag acgttgcaga ccccggatgt
     2821 aagctccggc cacggcgcgg tagcgcgaga gtgagaagat caaggtaacg tttatggact
     2881 tgccggcggc gatcatctcc tctatggccg cgagccccgg caccgtcgcc ggtatcttca
     2941 ccagcaggtt cggccggtcg accatctcgt gcagccgctt cgcctcggcc acggtcttcg
     3001 gcgtgtcgta ggcaatgtcc ggggagactt ccagcgagac gtagccgtcg agcttgccgg
     3061 actgctcgta gacaggcatg agcaggtcgc aggcgtcctg aatgtcctgc tttgcaagct
     3121 ccaggaagat ctccttcggg tcttcttctc tctgggaaag ctcctctagc tgctcgtcgt
     3181 agaggctaga ttcggagatg gctttctgga agatggtcgg gttcgaggtt acgccgacca
     3241 ccccgtcctc gatcatgcct tcgaggttcc cctccttgat atcgtcgcgg gagagggagt
     3301 ctatccagac actctgccca atctcctgca acttccgtag acgctcgttc accttcgcct
     3361 cctagatgcc ccgttcggag ctactgctcg tgccaccagt tccggtagtc tacaactcgt
     3421 ttcttacgag tatcttaagt tcttaggaat gtccctcctc cggggaagtg gcctcgaccg
     3481 ccggagtacc ggactcttca ctaaccctct cggagctgcc gagcaactcc agggccgcgt
     3541 tcgccacctt ctcggggatg atgccgagct tctccaggac cgtctcgccg ggcgccgaag
     3601 cgccgaagcg gttgatcccg atgctcgtgc ccttgaagcc gacgtagcgc tcccatccgg
     3661 aggtgatccc ggcctcgacc gagactcggg tctcgatgtt cggcggcagc acggagtcct
     3721 tgtaaccctg gtcctgggcg tcgaagatct cccacgacgg catactcacc acgcgagcat
     3781 ctacgccctt ctccgagagc gctttccgcg cttcgagcgc cacggaaacc tctgagccgg
     3841 tcccgatgag gatcacgtcc ggctctccac cgtcggcgtc ggccagcacg taggctccgc
     3901 gcagggctcc ttcggcagta ttcgggtcga ggaccgggac gttctggcgg gtgaggagca
     3961 gggctactgg accttcggcc tctagcgtgg cacgccacgc catcgaggtc tcgttcgggt
     4021 cggcaggacg gatgagggtc aggttcggta tggcgcgaag ggccatgtag tgctctatgg
     4081 gctggtgggt cgggccgtcc tcgcccagac ccacggagtc atgggtgtat acccaggcga
     4141 cctggaggtc catcagggcg gagagcctga tggccgggcg catgtagtcc gagaagatca
     4201 agaacgtcga gccgtagggc cggaccatcc cgccgtgtac ggctatgccg ttcaccgcgc
     4261 tgcccatccc gtgctcccgg atgccccagg ctatgttgcg tccggtgtgc gtcgaagaga
     4321 acataccgcc gttactgaac gtggtggagg tggactgcac gaggtccgcc gccccgccga
     4381 ccatcgtcgg ggtgtaatcc tggaaggcgt ccatgacggc ggccccggcc ttccgggtgg
     4441 cgagcttctc ggtctccgcg gggtcccagc ggggcagctt ctcctcgtaa ccgggcatgg
     4501 gcctgccgct ccaggctacc tcccactcct cggcgagttc cgggaactcc tcgctccagg
     4561 cggagaagcg gtcttcccat tcggcttcca ggtcccggcc cttttcggcc atgttcatgt
     4621 gctcgtagac ctcgtcggag acggcgaagt gctcgtcggg atccatactc atcgcttctt
     4681 tcgccagacg gatctcgtct tcccccagcg ccgagccgtg ggccgcggcg gtgtctatgg
     4741 cgttcggagc gggataggcg atatggctcc gcacgatgac gagagacggg cgctcttctt
     4801 cgtcgcgggc gttgattagc gcatcttcga tggcgtcaag gtcctcggag tcgtctacgt
     4861 gctggacgtg ccagccgtag gcctcgaagc gcttgccctt gtcctcgccg tcgaaggaga
     4921 gggccgtggt gccgtcgatg gtgatgcggt tgtcgtcgta gacatagacg agcttgccga
     4981 gggcgttctg gcccgcgatg gaggcggctt cctggcagac gccctccatc aggtcgccat
     5041 cggagcagat agcgtagatg tggtggtcca cgatctcgtg tccgatccga ttgtagcgcc
     5101 ccgccaaaaa ccgctcggcc atagccatcc cgacgccgtt ggcgaagccc tggcccagag
     5161 gtccggtcgt cgtctcaata ccaggcgtca tgaagtgctc ggggtggccg ggggtgcggg
     5221 attcccactg ccggaagttc ttcaggtcct cgatagtgag gtcgtagccg gtgaggtgca
     5281 ggagcgagta ctggagcatc gaggcgtggc cggcggagag tatgaatctg tcgcggtcct
     5341 tccaatccgg gttctccggg ttgtggcgca tgagcttggt gtagatggtg taggcgaccg
     5401 gcgccatgga catcggcgtt ccgggatggc cggaggcggc cttctcgact gcgtccatcg
     5461 ctagcgcccg gatggtattg atggagacgg cctcgatgtt cctggatttg agatccacgc
     5521 ttcacctttc ctcgctcgaa acagccagtg tgaagctacc attctatcca ctcatccaaa
     5581 cctcgcgcca acttttccga ggacgcaaaa ggccgggctc aaaacagagc ccggcctcaa
     5641 gacaggtcag gatccctcag aagggaatgt cgtcgaaatc ttcttcgttg atgtccacgt
     5701 cgtcgcgctg accgccctgc cgctggcctg cgccgcgccg attgccgcct ccaccgccgg
     5761 aggcgccgtc accgtcgctc cggccgccga ggaactggac gttgtcggcg actatgtcta
     5821 ccttgctccg cttcgagccg tcctgggact cccaggtgcg gtactggaga cgcccctcga
     5881 cgaggatggg gtcgcccttc ttcttgtagt tggcgatagt ctcgccgagc tcgcgccagg
     5941 ccgagacgtc gaagaagtcc acctcctcgt tcttagagcg cactcggttg acggccatgc
     6001 cgaactcgca gaccggtatg ccgctacccg tgaacctgag ctccggatct cgcgtgaggt
     6061 tacccgcgat tatcaccctg ttgaaactga ccacttcggc gtcctcctat tctcagtcct
     6121 cctgaagcta gtctacagaa acatccccgc ttctcaagag cctaaacttc gccggaaccc
     6181 ggcttccggg ccgtcacgag ccgcgcgggc gggaagatgt cgaagcggag cttggaccgg
     6241 ctgacttccc gcatgccgga ctcgcgcagc atcttcgcgt actcgccggt atcccagatg
     6301 tccagaatgg cgatgcggcc tccgggcttg agggttcttg ccagggcgag cagcgccgcc
     6361 gcacgtcctt ctcgctcggg gatgttgtgc aggacgaggc tggagaggat cacgtcgaaa
     6421 gagtcgtcct cgaagggcaa cttttgcagg tcggcctcga cgacctcgat ccgctcctca
     6481 actccctcga tgcgggcgtt ggcaagcgcc gcctccggac cgttgcccga ctgcttcctc
     6541 ttccgccaga tgtccacccc aacagcccta ccacctatca ggcgcttggc cgccccgatc
     6601 agcatgagcc cccgaccgca accggcatcg aggacggcct catcgccgcg ccaggggatc
     6661 gagccgagga tgcggtcgcg ctccttgagc ttgccgcgac ggctgctcag tatcatcaac
     6721 gcggcggtac ccagaccgca gatgccccag atgacgccga tgcccactac gagagcagca
     6781 agggccgggc ggctcccggc gaaagcgtag tagaaggcgc cagcggagag cgaggagata
     6841 cccgccagcg cgaagttccg cacgatatcc ggcgcgtcta tcccgtaatc cgcgcgctgg
     6901 ttcatgcgtt cacggcttcg tcacggccac gtcaaccttc tggtctttta tggtgacgat
     6961 gatgtctccg tcctcgtcgt tgcggaagac tttcgcccca tactcccgca ggcgggcaag
     7021 cgtctcgggc gtggggtggc cgtaggaatt ttcgccgacc tggataacag ctatcttggg
     7081 ccggaaagcg ttgaggaata gcggcgtcga ggaggtattc gagccgtgat gatccacctt
     7141 gaggactgtt acggggcctg tgtacggtcc gttcgccatg tactcttcct cttttgcctc
     7201 agcgtcgcca gcgagcagga tacgggccgt tccgtaggtc aggagaagag agacagagtt
     7261 gtcgttcgag tcttcgaaga gacctccgcc agcgtccggc ggaggagcga tcaccgtgat
     7321 gtccactccg ccccagtcca ttagcttccc agcgcgcgct tcctccactc ttgatccttc
     7381 ggcctgtacg cggcgcagga aggtattgta agtaatggtc gacttgtcgg tgtcaccgga
     7441 gaggtagact gtcgctacgt cgaaggcgtc gaggacttcg ggcagaccac cgatgtggtc
     7501 tgcatcgggg tgggttgcta ccatgccatc gagatccctc acgccccggc tgcgcaggaa
     7561 gtccaccacc tccggtcccg cctccgagcg tccggcgtct atgaggtagt tctcgccgcc
     7621 agcctgcacg agcaccccgt caccctggcc cacgtcaata aaactgacgg agagcgagcc
     7681 tgagggtggg ggcgagacgc cgccgaggga cctgccggag gcaaggtccc gaacccccgc
     7741 gcacccggat gccagaagga gcaggcagga caagagcagc gggagcgtcc cgcgaagcgc
     7801 ccggcgaagc gtctcgccga gcgggcgccg gttggtcttg gctattcccg gtcctcggga
     7861 gtgaggcggt cgatccactc gtcggcctcc gcgcgggtga ggtcggttag cggcttgccg
     7921 agccggctct ccagccgctc gacgccgttc tcgccgcgca tctcgaccgc gagggtcttc
     7981 aacaggtcca cctggctctt tcgggccttc agcgtgccgc tcctgctctt cccggcgggc
     8041 ggcttctcgg caggcgcagc gggagcctcg gcctgcccgt ttacggtagc ctctttggcg
     8101 gcgcgcgagc tctgcgccgg gtcctcttcg cggcgttcgc tctggcgtcc gccatgtatg
     8161 ggggtgggct ggttcctggt gcgggtgaag ttatcggcgg gcggagcgtc gtcgccgtcg
     8221 gagagctctt cgagggcggt cgcgccgacg ttgacggcgt cgcgcagagc gcgcgccttg
     8281 gcgcgagtct cggccatcct tatgaggtgc ggagcgatgt tgcgtccgac gttgttagga
     8341 gaagcgtctc ctatgccgga gaagacgcgg tcgtcctcca tgcgaacagt cgccttcacg
     8401 acggctacgt tgccgttcga gtcgtcgggg acctggatca actcggtgtc tatccccttc
     8461 aagccgcgat tgtgcgcctc gtctagcaga cccgcgaaca ggacgtactg tttcccctgg
     8521 cgcgttatca tgaactcgtc tctcatatca atcctccctg tgcctgataa tagctggctg
     8581 tcagacagaa ttcctcacct ttttggagac aaccctaaat tctttagcgg ctccgtcttt
     8641 agctgctccg ggcccgggag cgagtccgtg ttccaacaaa tacgccgagc agcgtcccga
     8701 ccagcgcgat agcgagcgac ccgattccaa gcaccgtgac ctgaggctcc tgggtcacgt
     8761 accatacgat gacgatcacg gccggcgcga cgagccacaa gacgtagacg ggacgcccct
     8821 catcccatac aaatcccgca acgaacgcta ctaggagata ggcaaagaag gtcatcccgt
     8881 aggcgagagc atagtctccc agcccggagc tatcaccgaa cagcgtcacg aagaccagga
     8941 aaaaggccgg gacgacccca accgctaaca gaatcaagcc ccggaccacc acgttcgcct
     9001 ctctacggat tacctggaga catctctccg agctctaatc accggtctct cgcagacaac
     9061 gctctcaaag accgcgccgc acttccgcct cgcggtagtc ttcgccttcc agcgagatcc
     9121 aatcgcacat cccgatgatc ctgctggctg tcctctcccc gagctggctc gcgaggtcct
     9181 tcgggccgat gttcgacgtg aagatggtcg gaagctcttc ccggtaacgg tggttgacga
     9241 tgacgaagat tcgctcccgc acccactcgt tcgcgcgctc ggcgccgagg tcgtcgagga
     9301 gcaagaggtc ggcgttcttt acggcggcca tccactcgtc tatgtcccgc cccggatcgt
     9361 tgtacgctcc gcgcatgttg tccagaagct ccgggacggt cacgaagagc gccggcaccc
     9421 gccggcgcga gatcaactcg ttcatcaccg cgaccgcgag gtgggtcttc cccgtcccaa
     9481 cgtcgccgca gaagtacaac ccccgcccgg tctcccggtt ctcctcccaa ctcttgaggt
     9541 acgtctggac cttctccagg gccctcgtgg agctgggcgg gtttgcgaag ggatcgaagc
     9601 tcgcgaaggt gaagcccttc atgcgctttg acaggccgct gcgctccttg agggtcgcca
     9661 cgcgcgagtc gcggcgcatg agctcccact cgcgttgctc gttcttctgc tcgcactcgg
     9721 gggtgcaggg gtggtaatac cattcgcccg gcttctcgta cttcttctgg agggtcggcg
     9781 ggaactcgac ccactcggcc ataagctccc ggccgcagtg cggacagacc tggtcgtcca
     9841 gcctcagcgc ctccgcttgc ttactccgtc tcgccgaaga gccactcata gccttcccgg
     9901 cgtcgggcag cagattgtct attcgctcca tcactcaccc cctctccctt tccgccttcg
     9961 cgcaacttca acgctccgcc ccggaccgcg ccgctctgct ccacgagcct caggcggcgg
    10021 tcctgctcga agcgctccac gtcctcgacc gtcttcacgc cccgctccac ccagcgctcc
    10081 aggatgctcc gcacgtagcc cacgcgccga gcgtcgcgct cgctggcgat cttaagagcc
    10141 tcccgcacca cctccggcga gagcccgtcc cgtccgcagt agccgttcag gaactccagg
    10201 atatgcggcg ccggcaggct tcccatgtat ctgaagtaat catccgccga gaccccctcc
    10261 ggctccgccc gtataacctc tatatcgtaa acgggcttct cctcggctgt ctcccgaacg
    10321 atcctggtgg ttctctcttg tttttgatct ttcgccgccg cagtcggcgg atgttccgca
    10381 acctccatcc aaccgtcccg ctcgacgata aagcccgtct ccgtgagagt ctcgaacgcg
    10441 gcttcgacgc tctccggagt ggagcgcagg agttcggcga cctcctcgac ctcgacggag
    10501 tcccggccga tgtccgggag gatgcggagc agctcgtaga gggcgacggc ctccgcgcct
    10561 atgtgcggca tccagcgcac gtagagccgg gcgcgctcgc cccgcgcccc acgccctccc
    10621 ggaggtgtga gccttactct ctccatcctg ccgccaagta taacggtcga gaggcgggct
    10681 ctctacggtc aatgctcacc aagacggttc cgtctagtct ttgccgcgaa gctcggtcga
    10741 agggaggccg cggtccgagg cgtctaagcc ggggatgagt ccctggctcg cggcgaggcc
    10801 gaagaagagg accaccacgg aaaagataat ggtcgccgtt ccgagactgc gggagcccag
    10861 gaaataaccc aggatgccca gaccggcccc tatcacaccg gaggtgacgc tggcgccgcc
    10921 gccacccagc acgatcacga ctccgtacac cgcgaacagg accccgatga cgcaagagat
    10981 cctgccggcc agcagcttcg cctgatcctc gcgctccgag tttgtgttat ttctgtctcg
    11041 gtcctcactc aaggacagcc ctccgcttcg tccgcaggtc catgcatcct agtataatcc
    11101 tcatcctgtg aggttcgagc gcaaactccc cggcacgaac gaggtgttcc agagagacgg
    11161 aacgccacgc cggacctacc gtcaggtcct ggatgagctt gcgcgcacgg ggacctcgcg
    11221 ctggcgggag aagttgaagc aggctcacgg tctcctgcta aacgagcagt acgccttcgg
    11281 catcaaggag ggcgataaga cgcatcctac cgattggatc ccccgcatcg tcccggccac
    11341 cgattgggag cgcctcgaac gcggtctaac ccagcgcctg cgcgccatca acgagttcct
    11401 gcggcgcctc gaagccggca gggaggaagt agtcccgaga gaggtcgtgg agagcagcat
    11461 cctctacgac actagctggc aggaccgctt cggccccgtg ccggtacgcc agatcgcctt
    11521 cgacgtggtg gcggtagaag gaccgtccgg cggctgggat tacgtcgtca tcgaagagaa
    11581 cgccaagatg cccgtagggc tcgccgccat gacccgccgg cgccggatga gcagtgaggt
    11641 cttcttcccg gagtcacacc gggaactgcc ggtgcggtcc ctcgacggct ggcttccgcg
    11701 tctcggcgac gctctacgcg ccgcctctcc gaagggcgcg gacgcaacgc tcgccgtcgt
    11761 ctccagcggt cccgctgacc agttctatct cgaccataat atctacgccg cggagatgga
    11821 cgccatcctc acagagacga aagacatcgg cgtggacggc gacgggtatc tcatgcacgg
    11881 accgacgggg cgccgcatag acgtgatcta cgagcgcgtc gacgaggacc gtctctacgc
    11941 cgaggtgccc ggcctcctcg aatgccacgt ctccggcaag acgcacgtcc tcttcgcccc
    12001 gaactccgag ataatcgacg acaagggcgt cggcgcgttc gtgccggaga tggtgcggac
    12061 atacctcggg gaagagccgc tgatacagaa cgctcggacg tggtcgctcg ccgtggagga
    12121 agatcgccgc tacgtcatgg atcacttcga ggaactggtg gtaaagagcc ggggcggcta
    12181 tggcggcaaa gaggtcatga tcggcccgga ggagccaaag gaatctatag agaagttccg
    12241 cagacaggtc gaggagaacc cggtcgagta catagcgcag gagatgatcg acttctccac
    12301 ccacgtcctc tgcgagccgg acgaggacga cttcctgctg cgcgactcct tcgccgacta
    12361 ccggatgctc gtcctctccc ccgacccgga agatccggac gttgtcgaga tggttccggg
    12421 ctccctgagc cgcgtcgccg cgccaggcaa gcacatcgtc aacatctcca gcggaggcaa
    12481 gatgaaagac acctgggtgc tcggggaggc aggacaatga actatggcga gatcatctcc
    12541 agcgccgttc ggctgacctg gcgcaacaag ttcctgtggt tcttcggctt cttcgcggga
    12601 gccggcacat cgttcaacgt ccccggcgga ggtagctctg gaggagatgg cctgggaggc
    12661 ggcctcccgg ctccactgcg gggcatcacg tcctgggtcc aggacaacct ggccctcttc
    12721 ctgacggtag tcataacgct ggcggtgttg ttcgttgtgg tcgggatcgc gctgtccata
    12781 atctcgcagg gcgcgctcgt tgagagcgtg gccgcgctgg accggggcga ggagcgccgt
    12841 ttcgggtcca cctggagagc ggggaccgcg aacttctggc gggtgctggg tctgaaggtc
    12901 ctgttcgtac tcatctcgct cgggctcgcg ctcgtgatga cgctggtgct ggtgctgccg
    12961 acagtgggca ttttcttcgc caccgactca acggcggcgc gggtcattgc cgtgatcttc
    13021 gcggtactgg cggggatagc gctgctactc ttgatcttcc ttcccctggc cgtgatcgcg
    13081 cagtgggccc ttcggcggct ggtggtggac cgggagcgca tcgtggaatc ggtccgcacc
    13141 ggctacgggc tcttccggcg caacatcggc cggagcctgc tcgtatggct cattcagctc
    13201 gggttgatgc tcggggccgg catcgtaatg ctcatagcgc tcattatcct cgggctcgta
    13261 ctggtcggtc cggcgttctt gctcgtccgg gcggagtaca ccgcggcggc gatagcgttg
    13321 ggcgccgtcg gaggcacaat cctgctggct gtcttcttcg tcctctccgc cgtcctcggc
    13381 accttcaact ccagctactg gacgctcgcc tacctgcggc tc
//
LOCUS       DASSMB010000252         6894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1343826, whole genome shotgun sequence.
ACCESSION   DASSMB010000252 DASSMB010000000
VERSION     DASSMB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1343826"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="VFJ72_14435"
     CDS             complement(<1..434)
                     /locus_tag="VFJ72_14435"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET7480700.1"
                     /translation="MTDTADQKMQEREYGFDTLALHGGQEVDSATTARAVPIYQTTSY
                     VFHDTDHAANLFSLSEFGNIYTRIMNPTTDVFEKRMAALEGGVGALAVSSGQAAETLA
                     ILNIAGAGDEIVSAAALYGGTYNLFHYTLPKIGINVKFVDSR"
     gene            complement(427..1578)
                     /locus_tag="VFJ72_14440"
     CDS             complement(427..1578)
                     /locus_tag="VFJ72_14440"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HET7480701.1"
                     /translation="MTLDPGAGASQAPARPLRSHHRIGSFEPELGGTLQDVTLAYETW
                     GELNASADNAVLIVHALTGDSHAAGGPSPEYRRGGWWEPMVGPGRAIDTDTQFVVCSN
                     VLGGCSGSTGPASLDPASGRPYGMRFPVITIRDMVRAQKRLLDDLGVKRLALVIGGSI
                     GGQQALEWAVEFPDSVEKAVPIAATGALGPQGLGMSEIGRRAIMADPDWQHGDYYGTG
                     RSPDDGLAIARMAGMMTYQSASGQWERFGRRPASRPALFEEHGGRFEIESYLHYQGKD
                     LVGRFDANSYLYLLRAMDIYDVALGYESDEEAYSRVEAEVLFVGVTSDWLFPAAEVRQ
                     SAERARNAGAKADYAEIDTLSGHDAFLKDWTELREAIGPFLNKQEERDD"
     regulatory      complement(1696..1817)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(1872..3302)
                     /gene="proS"
                     /locus_tag="VFJ72_14445"
     CDS             complement(1872..3302)
                     /gene="proS"
                     /locus_tag="VFJ72_14445"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7480702.1"
                     /translation="MTEAVEKLTKKSEDPSKWYLQLVRMAKLADYGPVRGTFAIRPYG
                     FEIWERVQADLDARFKATGHKNAYFPLLIPESYLKKEAEHVEGFDPELAWVTIGGREE
                     LEERLAIRPTSESIICDFYRNWIQSYRDLPVLINQWCNVLRWEKVTRPFLRTSEFLWQ
                     EGHTVHATAEEARAETLQMLGVYQDCFHEAMSIPVLKGIKSPSERFAGAVETFTCEAL
                     MGDGRALQAATSHDLGQNFARAFDITFLDENQERVHPYQTSWGFSTRTIGGLILVHGD
                     DRGLKIPPKLAPTQAVVVPIWRGNNKGEVRREAEALFSELKDSGLRMEVDLDEEHSPG
                     WKFNEHELRGVPVRVELGPKDIEKGQAVLVRRDTGEKEFVGRKELPDRLLDLMGEIQG
                     NMFRQADSFRRENTREAASYEDFREIIAEKRGFVVAPWDGTEETEQKIKEDTKATIRL
                     LPFEREEGKDLVSGKPGKTAIFARAY"
     gene            complement(3389..4405)
                     /locus_tag="VFJ72_14450"
     CDS             complement(3389..4405)
                     /locus_tag="VFJ72_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET7480703.1"
                     /translation="MESGVSLALVAIALAYAFDFANGFHDTANAVATIIYTKSMTSRN
                     AIIMSGVLNFLGAVIVGTAVAMIITEVIPADVVTMKLILAALIGGLIWNLGTWYYGLP
                     VSSSHTLVGSLVGAGLAAGWISGVQWGSLTKIILSLLVSPVLGIVLGWIVTRMTLPLA
                     GRRTSAKKPAADHPQLLKWMTILSGAAVSFSHGSNDGQKTMGVITLVLIAEFGRSPEA
                     GVPFWVVVTAAAAIGLGTMIGGWRIIRTVGEKISQVGITPVQGFGAQLSAALIILIGS
                     RFGAPVSTTHVLSSAVAGATIGEHTRKGLNFKVASNIFLAWIVTLPATALMAALIYFV
                     IAAL"
     gene            complement(4627..5409)
                     /locus_tag="VFJ72_14455"
     CDS             complement(4627..5409)
                     /locus_tag="VFJ72_14455"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HET7480704.1"
                     /translation="MEASGQARVWYQSFVDPDEQRPYIDRLRDHLDSVAEAGFSYDVA
                     GISPPDRYFHPITEFRIAGQVIRNALEAERQGFDAFIVGHFQEPGLNECKGAVDIPVV
                     GLGEAAMLHALTLGRKIGLITINPIFIPWHEDQITRYGLGQRIVGVRAVNTGVDDYMR
                     AFEDVDAYKGVLEQFRRQAEPLLEMGVEVIIPAGGLPMLLFSGESNFTVGGATVLNGI
                     TVAAKAAEMAVKLRRLDGTSVSRRSWFAKASSEAIREFEREQ"
     gene            complement(5411..6703)
                     /gene="serS"
                     /locus_tag="VFJ72_14460"
     CDS             complement(5411..6703)
                     /gene="serS"
                     /locus_tag="VFJ72_14460"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET7480705.1"
                     /translation="MLDLKYIRENAQAVQENCKNRGVEADVGLVVELADRRSALIGEL
                     NELKQRQNAMAKSIGQERDQEKRGRLIEESRGMKEEIPAKESELHGVEERLREEQLKI
                     PNMTHPDSPIGKDDTENVEIRRWGDIPDFSFEPRDHVQIGEALGIIDFDAGAKTTGSK
                     FYFLRGDAVLLELGLIRYAMDILMERGYEPTITPDLARDEALVGTGFIPRGPETQIYS
                     VEDSDLSLIATAEITLAGQHADEIVDESKLPIRYAGLSHCFRTEAGSHGRASRGLYRV
                     HQFTKVEMFAFTTPEQSESVHNEMVEIEERIFQGLGLPYRVVDICTGDLGGAAYRKYD
                     LEAWMPGRGEAGEFGEVTSTSNTTDYQSRRLKIRHRKEGGRPQLLHTLNGTALAISRA
                     LIALLEIYQREDGSVRLPESLVPYVGREVLSLSRTQRA"
     gene            complement(6788..>6894)
                     /locus_tag="VFJ72_14465"
     CDS             complement(6788..>6894)
                     /locus_tag="VFJ72_14465"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HET7480706.1"
                     /translation="FGAWVTAVVFTALDAAFLFVRIREEERALREIAG"
BASE COUNT         1133 a         2270 c         2163 g         1328 t
ORIGIN      
        1 tcgcgcgagt caacgaactt gacgtttatc ccgatcttgg ggagcgtgta gtggaagagg
       61 ttgtacgtcc cgccgtagag ggccgcggcc gagacgatct catcccccgc ccccgcgatg
      121 ttgaggatcg ccagcgtctc cgccgcctgc ccggaggaga ccgccagggc gccgacaccc
      181 ccttcgagcg cggccatccg cttctcgaag acgtccgtgg tcgggttcat tatgcgcgta
      241 tagatgttgc cgaactcgga gagcgagaac aggttggcgg cgtggtcggt atcgtggaag
      301 acgtaggacg tggtctggta gatcgggacg gcgcgggccg tcgtcgccga gtcaacctcc
      361 tggccgccgt gcagcgccag ggtgtcgaac ccgtactcgc gctcctgcat cttctggtct
      421 gccgtatcag tcatctcgtt cctcctgttt gttcaggaaa ggcccgatgg cctctcttag
      481 ctctgtccag tctttcagaa atgcgtcgtg gccgctgagc gtgtctatct ccgcgtagtc
      541 cgctttcgct ccagcgtttc gcgcccgctc ggcggattgt cgcacctcgg cggccgggaa
      601 gagccagtcg ctcgtgacac cgacgaagag cacctccgcc tctaccctac tgtatgcttc
      661 ctcgtccgac tcgtagccga gggccacgtc gtagatgtcc atcgcgcgca gcaggtagag
      721 gtaggagttg gcgtcgaatc ggccgacgag gtccttgccc tggtagtgca ggtagctctc
      781 tatctcgaac ctgccgccgt gctcctcgaa gagcgccgga cggctggcgg gcctgcgtcc
      841 gaaccgctcc cactgcccgc tagcgctctg gtaggtcatc atcccggcca tgcgggcgat
      901 ggcgaggccg tcgtcggggc tcctgccggt gccgtagtag tcgccgtgtt gccagtccgg
      961 gtcggccatt atggcgcggc ggccgatctc gctcatccca agcccctgcg gcccgagagc
     1021 cccggtggcg gcgatgggca cggctttctc cacggaatcc gggaactcca cggcccactc
     1081 cagcgcctgc tgcccgccga tggacccgcc tataaccagc gcgagccgct tcacgccgag
     1141 gtcgtccagc agccgcttct gggcgcggac catatcccgg atcgtgatga cggggaagcg
     1201 catgccgtaa ggtcggccgg acgcgggatc cagcgaagcc ggccccgtgc tgccggagca
     1261 gccgccgagc acgttcgagc acaccacgaa ctgcgtatcc gtatcgatag cccggcccgg
     1321 ccccaccatc ggctcccacc atccgccacg ccggtactcc ggcgaaggcc ctccagccgc
     1381 gtgcgagtcg cccgtgaggg cgtgaacgat cagcacggcg ttgtcggcgg acgcattcaa
     1441 ctcgccccag gtctcgtagg cgagcgtgac gtcctgcagc gtgccgccga gttccggctc
     1501 gaaggagcct atgcggtgat gggatctgag cgggcgggcc ggggcctgac tagcgccggc
     1561 cccagggtct agtgtcatac tcggtagtaa tgcagcctcc ttctatctcg tcgggtccta
     1621 cgtcgcggcg tccgccgtga tgtgcggcgt ctgccctacg cgcaacccgc ccgtacaaga
     1681 ggctgcccac ctctcttcac atcttccagg ccggatatga ccgccctgct ggacttggca
     1741 cctcgcggcc gatgttgtct tgccgctggt tgccgcggtt tctcggggcc agttccctcc
     1801 accgctctcg atgcgagtta gcgctaacgc tggatgctaa aacatcgagg ggcttatcgt
     1861 caagagcaat ctcagtaggc gcgggcaaat atagcggtct tgcccggttt gccggagacc
     1921 aggtccttcc cctcctcgcg ctcgaatggt aggaggcgga tcgtggcctt cgtgtcttcc
     1981 ttgatcttct gctcggtctc ctcggtgccg tcccacggcg cgacgacgaa gccgcgcttc
     2041 tccgcgatga tctccctgaa atcctcgtaa gacgcggcct cgcgagtgtt ctcgcggcgg
     2101 aaagagtcgg cctgccggaa catgttcccc tggatctcgc ccataaggtc tagcaggcgg
     2161 tccggcaact ccttccgccc cacgaactcc ttctctcccg tgtcccggcg cacgagcacg
     2221 gcctgaccct tctcgatatc cttcgggcca agctcgacgc gcaccggcac gccgcgcagc
     2281 tcgtgctcgt tgaacttcca gccgggcgag tgctcctcgt ccaggtccac ctccatccgc
     2341 aaaccggagt ccttcagttc ggagaaaagg gcttcggcct cgcggcggac ctcgccctta
     2401 ttgttgccgc gccagatcgg gacgacgacg gcctgggtcg gcgcgagctt tggcggtatc
     2461 ttcaatcccc ggtcgtcgcc gtggaccagg atgagcccgc cgatggtccg ggtcgagaag
     2521 ccccacgaag tctggtacgg atgcaccctc tcctggttct cgtccaggaa ggtgatatcg
     2581 aacgcgcggg cgaagttctg gccgaggtca tggctggtgg cggcctgaag cgcgcgtccg
     2641 tcgcccatga gggcttcgca ggtgaacgtt tcgacggctc cggcgaagcg ctcggaagga
     2701 ctcttgatgc ccttgaggac ggggatagac atggcctcgt ggaagcagtc ctggtagacg
     2761 cccagcatct ggagggtctc ggcgcgggcc tcctcggcgg tagcatggac ggtgtggccc
     2821 tcctgccaga ggaactccga ggtacggagg aacgggcggg tgaccttctc ccagcgcagg
     2881 acgttgcacc actggttgat aagtacgggt aggtctctgt agctctggat ccagttccgg
     2941 taaaaatcgc agatgatgct ctccgaagtt ggacggatcg cgagccgctc ctccagctcc
     3001 tcacgccccc cgatggtcac ccacgccagt tccgggtcga agccctcgac gtgttcggcc
     3061 tctttcttca ggtagctctc ggggatgagg agcgggaagt aggcgttctt gtggccggtc
     3121 gccttgaagc gggcgtcgag gtcggcctgc acgcgctccc agatctcgaa cccgtaaggt
     3181 cttatcgcga acgtgccgcg cacgggacca tagtcggcta gcttcgccat cctgacgagc
     3241 tggaggtacc acttgctcgg gtcttcgctc ttcttcgtca gcttctctac ggcttcggtc
     3301 atcaggggct cctctccggg tgcaaggtta cttgatcgag gagtatagcg cccatcctcg
     3361 cgccaatgtc gctacaccgc ggtcgcgcct agagcgccgc gatgacgaaa tagatcaggg
     3421 ccgccatcag ggcggtcgcg gggagggtga cgatccaggc caggaatatg ttcgaggcta
     3481 ccttgaagtt cagccccttg cgcgtgtgct cgccgatagt ggccccggcg acggccgagg
     3541 agagtacgtg cgtggtggag accggggcgc cgaagcggct gccgatcagg atgattaggg
     3601 ccgcggagag ctgggcgccg aagccctgta cgggcgtgat gccgacctga ctgatcttct
     3661 ctcccacggt gcggatgatg cgccagcccc cgatcatggt cccgagccct atcgccgcag
     3721 ccgccgtcac cacgacccag aacggcactc ccgcctccgg cgaacgtccg aactccgcga
     3781 tcagcaccag cgttatgacc cccatcgtct tctgaccgtc gttggagccg tgtgagaaag
     3841 agaccgccgc gccggagagg atcgtcatcc acttcaagag ctgtggatga tccgcggcgg
     3901 gtttcttcgc ggaagtcctg cgcccggcga gcggcagggt catgcgggta acgatccagc
     3961 cgaggacaat accgagcacg ggcgagacga gtagcgagag tattatcttg gtcagggagc
     4021 cccactgcac cccggagatc caaccggccg ccagccccgc tcctaccagg ctgccgacga
     4081 gcgtgtggga gctggagacc gggagcccgt agtaccaggt ccccaggttc cagatgaggc
     4141 cgccgatcaa ggcagcgagg atcagcttca tcgtcaccac gtccgccggg atgacctcgg
     4201 ttatgatcat cgccacggcc gtacctacaa taacggctcc caggaagttc agaacgccgc
     4261 tcatgatgat ggcgttgcgg ctcgtcatgg acttcgtgta gatgatggtc gccacggcgt
     4321 tggcagtgtc gtggaacccg ttagcgaaat cgaaagcgta cgccagagct atggcgacaa
     4381 gggctagaga cacgccagac tccaccgttc gccctccttc cccgaaaccc cgctgagata
     4441 cttcatcacc gcgagtatat tctcacaagt gtcaagtccg tgtccgcgcg agggctgctt
     4501 cttcgcccaa gcggcgacaa cgcctatcgg gccgcggcct ctcgctcctt gcatacgcaa
     4561 gaagtccgcg gttcgggcaa acgcgccggg ccgtccttgc ggtgccggat cttctggagg
     4621 cgctggtcac tgctcccgct cgaactcccg tatcgcctcg gacgaggctt tggcgaacca
     4681 ggagcgtcgg ctgacggagg ttccgtcgag acggcggagc ttcaccgcca tctctgcggc
     4741 tttggctgcg acggttatgc cgttaaggac ggtagcgccg ccgacggtga agttgctctc
     4801 tccagagaag aggagcatcg gcaggccgcc ggcggggatg atgacttcga cgcccatctc
     4861 caggagcggt tcggcctgcc ggcggaactg ttcgagcacg cccttatagg catccacgtc
     4921 ctcaaaggcc cgcatatagt cgtccacgcc ggtgttgacg gcgcgaacgc cgacgatcct
     4981 ctggccgagg ccatagcggg tgatctggtc ctcgtgccag gggataaaga tcgggttgat
     5041 ggtgatgagc ccgatcttgc gtcccagggt cagggcgtgg agcatggcgg cctcaccgag
     5101 tccaaccacc gggatgtcga ccgctccctt gcactcgttg agacccggtt cttgaaaatg
     5161 accgacgatg aaagcatcga agccctgcct ctccgcttca agagcattgc ggatgacctg
     5221 cccggcgatc cggaactccg ttatagggtg gaaatatctg tccggcggcg agatacctgc
     5281 cacgtcgtag gagaacccgg cctccgcgac cgagtcgaga tgatccctca agcggtcaat
     5341 gtacgggcgt tgctcgtcgg ggtccacgaa gctctggtac cagacccgcg cctgcccgga
     5401 cgcttccacc tcaagccctc tgagtcctgg aaagagacag cacctctctg ccgacgtaag
     5461 gtacgagcga ttccggcaag cgcaccgagc catcttcccg ctggtagatc tccagcagcg
     5521 cgatcagggc gcggctgatg gcgagcgccg taccgtttag ggtatgaagg agctgcggac
     5581 ggcccccctc cttgcggtgc cggatcttca atcgccgcga ctgatagtcg gtggtgttgg
     5641 aggtgctggt aacctcgcca aactcgccgg cttcgccgcg tccgggcatc caggcttcca
     5701 ggtcgtactt gcgatacgcc gcgccgccga ggtcgccggt gcagatatct acgacacggt
     5761 acggcaggcc gagcccctgg aagatccttt cctcgatctc gaccatctcg ttgtggacgc
     5821 tctcggactg ctcgggcgtg gtgaaggcga acatctcgac cttcgtgaac tggtggaccc
     5881 gatagaggcc gcggctcgcg cgtccgtgag aaccagcctc cgtgcggaag cagtgggaga
     5941 gtccggcgta gcggataggg agtttgcttt catccacgat ctcatcggcg tgctgcccgg
     6001 cgagcgtgat ctcggcggtc gcgatcaggg agaggtccga gtcctctacg gagtagatct
     6061 gggtctccgg ccccctcgga atgaaccccg tcccaacgag cgcctcgtcc cgcgccaggt
     6121 ccggtgtaat agtcggctcg tagccgcgct ccatcaggat gtccatcgca tagcggatga
     6181 gcccgagctc tagcagcacc gcgtcgccgc gcaggaagta gaacttgctc cccgtcgtct
     6241 tagcgcccgc gtcgaagtct atgatgccga gggcctcgcc gatctggacg tggtccctcg
     6301 gctcgaagct gaagtccggg atgtcgcccc agcgccggat ctcgacgttc tcggtgtcgt
     6361 cctttccgat gggcgaatcg gggtgggtca tgttggggat cttgagttgt tcctcgcgca
     6421 gccgctcctc gaccccgtgc agctcggact ccttggcggg aatctcctcc ttcatccccc
     6481 gcgactcctc gatgagcctc ccccgcttct cctggtcccg ctcctgccca atgctcttcg
     6541 ccatcgcgtt ctggcgctgc ttcagctcgt tcaactcccc gatgagcgcc gagcgccggt
     6601 cggccagctc cacgaccagc cccacgtcgg cctcgacgcc gcggttcttg cagttctcct
     6661 ggacggcttg cgcgttctcg cggatgtatt tgagatctag catggtggct ccctccggtc
     6721 gccggatatc agctttcagc ggtccgtttt cggctgtttc gtagtacctg actgctggcg
     6781 gctaatatta tcccgcaatt tcccgcagcg cccgctcctc ttctcgtatc cgaacgaaca
     6841 ggaacgcggc gtcgagcgcg gtgaagacca cagccgtaac ccaggctcca aaga
//
LOCUS       DASSMB010000253         6800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_342525, whole genome shotgun sequence.
ACCESSION   DASSMB010000253 DASSMB010000000
VERSION     DASSMB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342525"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VFJ72_14470"
     CDS             complement(<1..856)
                     /locus_tag="VFJ72_14470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7480707.1"
                     /translation="MSETLVIVGASLAGGSAAATLRQEGFDGRVILIGAEPQPPYERP
                     PLSKEYLRGETPFEGALVQTADFYRKNGIETRFGVRAIHVDAAAKAVELDGGEHVSYD
                     GLLIATGARNRRVRFPGSDLGGIYELRTVADCDRIREEIAPGRKAVVVGMGFIGSEVA
                     ASLRQSGVEVAVVDRNKVPLRRVLGEEVGRVMAEIHRDHGTELIFEDTVAAFEGTRRV
                     EKLITHRGRRIECDFVVVGLGVEPVTELLAGTGVEIDNGVLVDEYCRTNIEGIYAAGD
                     VANHYHPVF"
     gene            complement(856..1164)
                     /locus_tag="VFJ72_14475"
     CDS             complement(856..1164)
                     /locus_tag="VFJ72_14475"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HET7480708.1"
                     /translation="MAEFAVVAGANELREGEMLAFEVRGTRIAVANVAGTFYAFDDTC
                     THLRCSLAGGDLEEATVICPCHGSEFDVRSGEVLQGPARRPVETYETRIEGGSLEVRG
                     "
     gene            complement(1198..1377)
                     /locus_tag="VFJ72_14480"
     CDS             complement(1198..1377)
                     /locus_tag="VFJ72_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480709.1"
                     /translation="MWRGASNVVMAAPFASGWLLRIAAPGDPGALAIIVSFVEAGLAS
                     LGGFLGGDSTSTRRM"
     gene            1766..2422
                     /locus_tag="VFJ72_14485"
     CDS             1766..2422
                     /locus_tag="VFJ72_14485"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET7480710.1"
                     /translation="MAAYWDRRFFESTRGRLVTLLRRASRTVDELAADLGMTGNGVRA
                     HLATLERDGLVRQGGSVRRSGGGGGKPAHIYELTPEARDLFPKGYEAVLGGLLDVLTE
                     GVGDEGSKALLREAGIRMAGELGLPLGSVQERLQAAVGVLNRFGGLAELEEHDGAFVI
                     RGYGCPLTAVVVEHPQVCDLVEALIAELTGMRVCECCERGEIPRCRFEVRSADADAQR
                     "
     gene            complement(2727..2981)
                     /locus_tag="VFJ72_14490"
     CDS             complement(2727..2981)
                     /locus_tag="VFJ72_14490"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET7480711.1"
                     /translation="MILCAITSRVPRILSDWEVLLEAHDLMEEKLPRRSMVKVGKLFT
                     MHKGLVAARYGALKEQKLHEVLERLQNLFALDRSLARQKT"
     gene            3197..4786
                     /locus_tag="VFJ72_14495"
     CDS             3197..4786
                     /locus_tag="VFJ72_14495"
                     /inference="COORDINATES: protein motif:HMM:NF006182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET7480712.1"
                     /translation="MLDTDQTITRARRNTIGDAVKRSAARNPSKDALVFGERRWSYAK
                     LDAGANRVANALLELGLKKGDRVAAYGTNSDAYVLLWLGCVKAGLIHVPINFALTGDE
                     LLYILDQSGSQALFYDPALRENVEKVRAEVGVTIRGTLRDGADVDILGFAQGPEEDSE
                     PDIELDDHDPAQILYTSGTTAAPKGAVLTHGALMAEYVSCIEALEYRNDDRALHSLPL
                     YHSAQMHVFLMPYLLIGATNFVVEKPDPERCTSIIEGERIGSMFLPPTVWISFLRHPA
                     FDSRDLSSLEKVQYGASIMPVPVLRELRERLPNPRFYNCYGQSEIAPLATVLRPEEHE
                     EERLASAGRPVLLVETRVVDPEMNDVASGEPGEIVHRSPHLMVGYWGKPEDTREAFEG
                     GWFHSGDLGYFDEEGYLYIVDRVKDVINTGGVQVSGREVEEALYDHEAVSEVAVIGLP
                     DELWIEAVSAVVALKSGAEATEELARGLKERAREHLAPFKVPKHVFFVDDLPRNGAGK
                     ILKRELRERFAERVSPPAGRN"
     gene            complement(4834..5432)
                     /locus_tag="VFJ72_14500"
                     /pseudo
     CDS             complement(4834..5432)
                     /locus_tag="VFJ72_14500"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
     gene            complement(5669..>6800)
                     /locus_tag="VFJ72_14505"
     CDS             complement(5669..>6800)
                     /locus_tag="VFJ72_14505"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480713.1"
                     /translation="LLFAARRYRESLEAARRTLSLASEAGDDYTVVRARMACANALLF
                     LRRPEDARKELEAAMPLAKRAGATLNLLFGTLTLDLLDTASGEFERSLEWARRGLDLA
                     EQLDNRHEVSLRLARIGLILFYKGEWREARKYLERGTEAARSVGPLLLCAWAPGYLGM
                     LCMAEGLWEEAKKRLEEAIGLAEQVRWPTPLRYVLSLRAMLDLLEGRPQAALARLGSL
                     ADFPDLDWLCATVLLTALAQAHLESGDVARAGEIADRAIAEAATMHNRVDMVEALRLK
                     GTALTHQGRGKEAAVVLEDALSLARSMPMPYAEARIIHEYGVLHARRGEHGQARELLC
                     AALDIFVGLGAAKDADQTRRELLVPGPLTIAPRPREAPSRGR"
BASE COUNT         1192 a         2246 c         2147 g         1215 t
ORIGIN      
        1 cgaagaccgg atggtagtgg ttggccacgt cgcccgccgc gtagatgcct tcgatgttcg
       61 tccggcaata ctcgtctacc aggacgccgt tgtcgatctc tactccggtc ccggcaagaa
      121 gctccgtaac cggctcgaca ccgagaccca ctaccacgaa gtcgcactcg atacggcggc
      181 cacggtgggt tataagcttt tcgacgcgcc tcgttccctc aaaagcggcc accgtgtcct
      241 cgaagatcag ttcggtgcca tggtcacggt gaatctccgc cattacccgg cctacctcct
      301 cgcccaagac ccggcgcagc ggcaccttgt tgcgatccac gacagccacc tccacgccag
      361 attgccgcag agaggccgcc acctccgaac cgataaagcc catgcccacg accaccgcct
      421 tgcggcccgg agcgatctct tcacggatgc ggtcgcagtc ggccaccgtg cgtagctcgt
      481 agatccctcc caggtccgag cccggaaatc gaacacgtcg gttccttgcc cctgttgcta
      541 tgagaagccc atcataggat acgtgttctc cgccgtcgag ttcgaccgcc tttgcggcgg
      601 cgtccacgtg gatggcccga accccgaacc gggtctcgat gccgttcttg cggtagaaat
      661 cggctgtctg caccaaagcg ccctcgaatg gggtctcgcc acgcaggtac tccttggaga
      721 gcggtgggcg ctcgtaggga ggttgcggct cggcgccgat caggatcacc ctcccatcga
      781 agccttcctg gcggagggtg gccgccgcgc ttcctcccgc gaggctggcg ccgacgatca
      841 caagggtctc ggacatcagc ccctgacctc caggctgcca ccttcgatgc gtgtctcgta
      901 ggtttcgacc ggccgccggg ccggaccctg gagcacctct ccgcttctca cgtcgaactc
      961 cgaaccgtgg caggggcaga tcacggtcgc ctcctccagg tctccccctg ccaaagagca
     1021 tcgcaggtgc gtacacgtat cgtcgaaggc gtagaacgtg ccagccacgt tcgcaacggc
     1081 gatcctggtg ccccgcactt cgaacgccag catctcgcct tcgcgcagct cgttcgctcc
     1141 ggctacgacc gcgaattcag ccatcgtttt caactccttt cagtaaggca gccttcatta
     1201 catcctccgc gtgctggtcg agtctccccc gagaaagcct cccagcgagg ccagcccggc
     1261 ctccacgaag gagacgatga tggcgagcgc tcccggatcg cccggcgcag ctatacgcag
     1321 cagccaacct gaggcgaacg gcgcggccat gaccacgttg ctggctccgc gccacaaccc
     1381 gaccgctttg gcgcgcgtgc cgggtgtatc gccagccagt cgatcaggcc gaacactgca
     1441 gcggcaagcc caccaatcac accagcggcg atcatccaga aggagatcac cgaccatttc
     1501 ccgctacccg taaggagatg aacgatacga agactaccga tgtcgtaagc aaccccagcg
     1561 aaaacgggat cagtatcggg tgccccatca cccggaccct gctctccatc actctcttct
     1621 ccttctccaa cccatccgcg gcccgagcct ggcctcttct ggcacgatac acctgtacca
     1681 ataataaatc aagtaaatcc ttgaaatatt tgagtaactc tatatcttct gctacagtgt
     1741 ggatgtcaca gagaagggat agcgtttggc ggcttattgg gaccgcaggt ttttcgagag
     1801 cacgcgtggt cggctcgtca cgcttctcag gcgtgcgagc cggacggtcg atgagcttgc
     1861 ggcggatctg gggatgacgg gcaacggggt tcgggcgcac ctggcgacgc tggagcgtga
     1921 cgggcttgta cggcagggtg gttccgtgcg tcgttccgga ggaggaggcg gcaagcctgc
     1981 gcacatttat gagctgacgc cggaagcgcg ggatctgttt ccgaaaggct acgaggcagt
     2041 gcttggcggg ttgttggatg tcctgactga aggtgttggg gacgagggat ccaaggcgct
     2101 gctaagagag gcggggatac gcatggcggg tgaactcggc ttgcctttgg gcagtgtcca
     2161 ggagcgtctg caggcggccg tgggtgtgct caacaggttc ggcggcctcg ccgagctcga
     2221 agagcacgac ggcgccttcg tgatccgcgg ttacgggtgt ccgctgacgg cagtggtggt
     2281 agagcatcct caggtttgcg acctggtcga ggcccttatc gcggagttga ctggcatgcg
     2341 ggtctgcgaa tgctgcgagc gcggggagat cccccgatgc cgcttcgagg tgaggtcggc
     2401 tgacgctgat gctcaaaggt agccacgcca gccgtcggga gagcgcgacg agcgcgacgg
     2461 tcacggacgg gcgaacctct gcttgaatgc ttgctcggcg gcgcggtggc cttctccggt
     2521 gagcgtgacc gacctagtca ttatgttggg gtcggagatc aggccgcggt ggtgcaaccg
     2581 gtacacgaag tcccggccga aggtcttcca tgctcgggcc tgttcgcggt cgtcgaagtc
     2641 attcgaccag agcagagcca gggtcgcctc gtcgatctcg tccgggcggg ataccccccc
     2701 ttccgcgcct gcggtgtcgc ccgcggttac gtcttttgtc gcgccaacga gcgatcaagc
     2761 gcgaacaggt tttgcagcct ttcgagcacc tcgtgcagct tctgctcctt tagtgcgccg
     2821 tagcgagcgg ctacgagccc cttgtgcatg gtgaagagct tgccgacttt gaccatgctc
     2881 ctcctgggca gcttctcctc catcaggtcg tgcgcttcga ggagaacctc ccagtcggag
     2941 aggattcgcg gtaccctgga ggtgatcgcg cagaggatca gatcctcccc gtcgaatccg
     3001 tcccaactat cattaggagg agatcgtcct tacgcatgat ccgcccgcga acacgcaaca
     3061 gctccagggg agctatcgct ttagggaacg cttaggagat ccggccgaaa ccccttgcgg
     3121 tagggtaatg cttgagaggc gcgtgggttg ggagtgggtt aggatttcct caacaaggcc
     3181 gccgaggagg tcccagatgc ttgatacgga ccagacgata acccgcgccc gccgcaacac
     3241 catcggcgac gccgtgaaac gctccgccgc ccgcaatccc agcaaggacg cgctcgtctt
     3301 cggcgagcgc cgctggagct acgctaaact ggacgccggg gccaaccgcg ttgcaaacgc
     3361 tctgctggag ttagggctta aaaagggcga ccgggtggcc gcgtatggta ctaactccga
     3421 cgcgtacgtc ctgctctggc tggggtgtgt gaaggcggga ctgatccacg tccccatcaa
     3481 cttcgcgctt accggcgacg agctactcta catactcgac cagtccggct cccaggcgct
     3541 cttctacgac ccggcgctaa gagagaacgt cgagaaggtg cgggcggagg tcggtgtgac
     3601 gatccgcggc accctgcgcg acggggcgga cgtggacatc ctgggcttcg cgcaaggtcc
     3661 agaggaggac tccgaacccg acatcgagct cgacgaccat gacccggcgc agatactcta
     3721 cacttccggg acgaccgcgg ctcccaaagg cgcggtgctc acgcacgggg cgcttatggc
     3781 cgagtacgtg agctgcatcg aggccctgga ataccgtaac gatgaccgcg ctctgcactc
     3841 cctgcccctc taccactcgg ctcagatgca cgtcttcctc atgccgtatt tgctaatcgg
     3901 ggcgaccaac ttcgtggtgg agaagccgga ccccgagcgg tgtacctcga tcatcgaggg
     3961 cgagcgcatc ggctccatgt tcctgccgcc gacggtctgg atctccttcc tcagacaccc
     4021 ggccttcgac tccagggacc tctcaagcct ggagaaggtg cagtacggag cctcgatcat
     4081 gcccgtgccc gtgctgcggg agctgcgaga gagactgcca aacccccgct tctacaactg
     4141 ctacggccag tccgagatag cgcccctggc gaccgtcctg cgtcccgaag agcacgaaga
     4201 agagaggctg gcctcagccg ggcgtcccgt gctcctggtc gagactcgcg tcgtggaccc
     4261 cgaaatgaac gacgtagcga gcggcgagcc cggcgagatc gtccaccgct ccccgcacct
     4321 catggtcggc tactggggca aacccgagga tacgcgggag gccttcgagg gcggctggtt
     4381 ccactccggc gacctcggct acttcgacga ggagggctac ctctacatcg tggaccgggt
     4441 caaggacgtc ataaacaccg gcggcgtgca ggtctccggc cgcgaggtcg aggaagccct
     4501 ctacgaccac gaggcggtct cggaggtcgc cgtcatcggg ctgccggacg agttgtggat
     4561 cgaggccgta agcgccgtcg tcgccctcaa gagcggcgcg gaggcgaccg aagagcttgc
     4621 cagggggcta aaggagcgcg cccgcgagca cctcgccccg ttcaaggtac ccaagcacgt
     4681 cttcttcgtg gacgacctgc cgcgcaacgg cgccggcaag atactaaagc gcgaacttag
     4741 ggaacggttc gccgaacgcg tctccccgcc ggctggccgg aactgactgc aggagcccgc
     4801 cctgccaaac tttttcctta cgagttcccg gcatcactgg ccggaagggc tcgtccaggg
     4861 gagaaaggct ccgacccacc cccgcgaatc cctaaagagg ccgacgccgt tgagcgtccc
     4921 ggtgaggatc acgtctccgg gctccagcgc gacggactgg gagacgaaag ccacgagctg
     4981 cgccacggtg aagaccatct ccttggtgtt tgagtcctaa agcgtctcgc cgttcacccg
     5041 gcacactacc tgcaggtctt gcaggttcgt cacctcctcc cggctcacca cgaccggccc
     5101 cagggggagg aaggtgtcta tggccttgcc tcgggtccac tgcctgccct cgcagaactg
     5161 caggtcgcgc gcggtgacgt cgttggcagc ggtgtagccg aagaccgcgt ccagggcgtc
     5221 ttcctctctc aggcaacgtc cccctcgccg attatcacgg cgagctcggc ctcgtagtcc
     5281 acctgcttcg tgaggttttt gggccagcgg atctcctctt ccggccctat tacgtccgtg
     5341 gggaatttgg ggaacagaac cggaactccg ggcgaggccc gtagcgttgg gggcgctggc
     5401 gtgtagatgg gcgtcagaga agcataagag atccccggtt tctatggcca cccgcagttc
     5461 cgaggcgaca ctcacgggct ccgctggctc cggcaccaaa ggagtaggtt tgccggctcg
     5521 gagctcttca aggtcccagc gtcccagcct gcgctggtgt tcttcagggg cttctcccag
     5581 tccgggtaga aggcgatggt gcgcccggcg gttatggggt agaccggcgc ccaccagttg
     5641 gtttgcgagt agacgtcagg cgtctctgct accgtcctct tgatggagct tcccgtggac
     5701 ggggagcgat agttaatggt cccggaacca gaagctcccg cctggtctgg tcggcgtctt
     5761 tcgccgctcc tagacccacg aagatgtcca gtgccgcaca gagcagctcc cgcgcctgcc
     5821 cgtgctctcc ccggcgagcg tggagcaccc catactcgtg gatgatgcgg gcctcggcgt
     5881 agggcatcgg catggagcgt gccagggaga gcgcgtcctc cagcaccacg gccgcttcct
     5941 tcccacgtcc ctgatgtgtc agcgccgttc ccttgaggcg tagcgcctct accatatcca
     6001 cccggttatg catcgtcgcg gcctccgcga tggcccggtc cgcgatctct ccggctctcg
     6061 ccacgtcgcc cgattcgagg tgggcttgcg ccagcgccgt cagcaggacg gtggcgcaga
     6121 gccagtcgag gtcggggaag tcggccagag atcccaggcg ggcgagcgcg gcctgtgggc
     6181 ggccctccag gagatcaagc atcgcccgca gcgacaggac gtaacgcagc ggggtgggcc
     6241 accggacctg ctccgcgagc ccgatggcct cctccaggcg cttctttgcc tcctcccaga
     6301 gcccttcggc catgcacagc atgcccaggt accccggcgc ccaggcgcag aggagcaggg
     6361 gacctaccga acgcgccgcc tccgtccccc gctccaggta tttgcgggcc tcccgccact
     6421 cgcctttgta gaacaggatc aggccgatcc tggccagacg gagcgagact tcgtgccggt
     6481 tgtcgagttg ctctgctagg tcgagccccc gcctggccca ttcaaggctg cgctcgaact
     6541 ccccactggc cgtatccaag aggtcgagcg tgagggtgcc gaagaggagg ttgagagtgg
     6601 cgccggcccg cttcgccagc ggcatcgccg cttcgagctc cttgcgggca tcctcgggac
     6661 ggcgcaggaa gaggagggcg ttggcgcacg ccatcctggc ccggaccacc gtgtaatcgt
     6721 ccccggcctc gctcgcgagg ctcaacgtcc gccgggccgc ctccagcgac tcgcggtagc
     6781 ggcgggcggc gaagaggagg
//
LOCUS       DASSMB010000254        16720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1087557, whole genome shotgun sequence.
ACCESSION   DASSMB010000254 DASSMB010000000
VERSION     DASSMB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 16720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16720
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087557"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VFJ72_14510"
     CDS             complement(<1..878)
                     /locus_tag="VFJ72_14510"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7480714.1"
                     /translation="MTEARNGTRPLEGVRVVEMGSLLAGPFCGQLLADFGAEVIKVEP
                     PGNGDPMRVWGRHRKEGRTLWWPIIARNKKSVTLNLREEEGQDLARKLIAEADVVVEN
                     FRPGTMERWGLGYEKLSEINPGLVMVRVSGFGQTGPYREQAGFGAIGEAMGGIRHVTG
                     FPDAPPPRTGISLGDSLAATFGALGALTALYHREAHGGKGQVVDVGIYEAVLALMEST
                     IPEYALTGHVRGRTGTVLPFVAPSNIYPTKDGDYALIAGNADTVFGRLAKALGHPEWA
                     EDEKFATHHARGENME"
     gene            complement(875..1576)
                     /locus_tag="VFJ72_14515"
     CDS             complement(875..1576)
                     /locus_tag="VFJ72_14515"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7480715.1"
                     /translation="MGAALRRGEERPGSSAVFERLRRMILEGEYGPRERLIEEQLAER
                     LGVSRTPIRQALTMLEAEGLVEIEPNKGAMVCTFGVDEVWDIYDLRAVLEGHAARRAA
                     ERIDERGLVRLRELAGEMENLVEKSFEEREEEIRLLVTLNNEFHGTIVEASRNRRLER
                     MIRRTVELPLVFKAFFWYTPRERMVSNHYHRQILGALEKGDGERAEIVMREHVYEGRD
                     FVIHALEEDRKGEKQ"
     gene            1760..2146
                     /locus_tag="VFJ72_14520"
     CDS             1760..2146
                     /locus_tag="VFJ72_14520"
                     /inference="COORDINATES: protein motif:HMM:NF026182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HET7480716.1"
                     /translation="MPFYQFTVPADSPSTGLKADIATAVTRVHTEVTGAPARYVSVSF
                     HEVPPGSIFAAGEQVSQGRLVGIIRSGRTQEIKRVLITGLATAWSEVTGEPMEGFALF
                     ILDRPGSNIMDEGHSLLPLLVAGRAV"
     gene            complement(2104..3645)
                     /locus_tag="VFJ72_14525"
     CDS             complement(2104..3645)
                     /locus_tag="VFJ72_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7480717.1"
                     /translation="MVQPPTNEHLRELADRFGLRLDDAAATSFRGLVEGTLGSYRRLD
                     QLVEPELPVRYPRSRSRRPEPEENTLGAWYQKCSIEGAADGQLSGKRVAIKDNVCVAG
                     VPMMNGTSTLEGYVPEFDATIVTRILDAGGEIVGKAVCEHLCFSGGSHTSDSGPVLNP
                     HDHTRSAGGSSSGSAALVAAGEVDMAIGGDQGGSIRIPSSWCGVYGLKPTYGLVPYTG
                     VFPIELTLDHTGPIAASVSDVALLLGAIAGEDGHDPRQKDVRVGDYGSLDGGASGLRV
                     GILTEGFGWPGLSEDDVDEAVREAARAFGEMGAKVSEVSVPLHRDGIHIWNGIAIEGA
                     TELMVRGNSMGTNWKGHYSTSLLDAYARGRTTRAADLSETVKLTMLVGEYMRESYHGR
                     YYAKAQNLGRKLAAAYDAALESVDLLVIPTLPMKATPIPSADASREEIVTRALEMIPN
                     TAPFDVTGHPAMTVPCALSDGLPVGMMLIGKKWDEETILRAAHSFEEAGTYTARPATR
                     SGSSE"
     gene            complement(3765..5729)
                     /locus_tag="VFJ72_14530"
     CDS             complement(3765..5729)
                     /locus_tag="VFJ72_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7480718.1"
                     /translation="MTVGKLLVANRGEISVRVIRTCRELGIRSVAVYSEADERAMHRR
                     LADEAYAIGPAPAPESYLNIERLVEAIERSGAAAVHPGYGFLSESAAFARAVREAGAV
                     WVGPPPEAMEAMGFKVRAKEIARRADVPTVPGYDGSDDSEERLAEEAERIGYPVLVKA
                     SAGGGGRGMRAVTNPKEFAEAVRGARREAEAAFGDGSVFLEKLLEDPRHVEVQIIGDD
                     HGNVVHLYERECSIQRRHQKVVEEAPSPALSPELRETICDAAVRLAREAGYSNAGTVE
                     FLLDGEDFYFLEMNARLQVEHPVTEMVAGLDLVRLQLAVAAGDALPFRQEDVTLRGSA
                     IEARIYAEDDAGMPVGGKLLDLILPQGAGVRNDTGFDVGDEVPINYDSMLAKLIVHAP
                     DRSAAVRRLRRALDDYSVLGVPTNLPLLDRIAGHPAFAAGETTTGFLDQHALADPAQK
                     DIPRTALLIGAAADLSRSATSGDAFTLGPWRMMGALQIRYDVCGDTRAVEAARAGPRT
                     LKLTVTEETSTLEVLHVTDGRIRALVDGESAEAEFAADRSGLTLSFGGETYRLARPAP
                     PDVDEAGPGGDSAGAGLTAPMPGTIVKIVASEGDEVEEGQLLLVLEAMKMEQPVTAPH
                     AGTVTSLPYGEGSLVPGGAVLAEIERPESE"
     gene            complement(5726..6529)
                     /locus_tag="VFJ72_14535"
     CDS             complement(5726..6529)
                     /locus_tag="VFJ72_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7480719.1"
                     /translation="MADYRNSYEHLRRTDEGPVTTVSLARPDSRNALNAPLIEELTAC
                     FEEVSDDERARVVVLTGEGRSFCAGADIGYMRDTANLSYEKNLEDARRLAAMFRALDE
                     CPKPVVAKVRGAAMGGGAGLVAASDIVVAAEGARFAFSEVRLGIAPATIAPFVVRKIG
                     YSHARSLFLSGERFGAERAREIGLVHEVVAEDALNSAVSGKVDGLLAGGPYAQAAIKM
                     LLREIETVEPMDALALMSPLISELRTGVEGQEGLGAFLEKREPRWRENP"
     gene            complement(6532..8139)
                     /locus_tag="VFJ72_14540"
     CDS             complement(6532..8139)
                     /locus_tag="VFJ72_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET7480720.1"
                     /translation="MSKLRSHIDPRSEDFRRNAGAYENVLSELRERSAEARRGGGEKA
                     RKRHRERGKLPVRERIDLLLDPGTAFLELSPLAAHGMYDGRVPAAGIVTGIGRVSGRE
                     VMVVANDATVKGGTYYPMTVKKHLRAQEIAEQNHLPCVYLVDSGGAFLPLQDEVFPDR
                     EHFGRIFYNQARMSAKGIPQIASVMGSCTAGGAYVPAMSDEVVIVKGEGTIFLGGPPL
                     VKAATGEEVTAEELGGADVHTRRSGVADHFAEDDRHALAIVRSIVENLNTQKKPPWPI
                     EEPEEPLHDIEELYGIVPTDYRQGYDAREVIARIVDASRLHEFKPLYGETLVCGFARI
                     MGHPVGILANNGILFSESAQKGAHFIELCCERGIPLVFLQNITGFMVGKEYEAGGIAK
                     DGAKMVMAVASANVPKFTVIVGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGVQA
                     ANVLLTVQEENLKREGKEMNEDERTEFRRPILEKYEDEGNPYHSTARLWDDGVIDPVD
                     TRMVLALGLSAAANAPLEETTFGIFRM"
     gene            8326..8856
                     /locus_tag="VFJ72_14545"
     CDS             8326..8856
                     /locus_tag="VFJ72_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7480721.1"
                     /translation="MKRTTLTAVTIALLISLCASTALAATAATSGADTLRGTRSVDEL
                     RGLGGGDTLTALAGDDTLRGDGGRDLISGGGGNDTIYGGPGDDASTHIFAGLSGGAGN
                     DRVYGGGGNDYIIGDTGRDLLSGGDGDDYIASTSGESYDEPTSKDNISCGPGFDTVSA
                     DTADYVAPDCEDVTRF"
     gene            complement(8907..9266)
                     /locus_tag="VFJ72_14550"
     CDS             complement(8907..9266)
                     /locus_tag="VFJ72_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET7480722.1"
                     /translation="MPLYALNLFDLAENDLYRRYSRRSVEAVGKHGGRVVALGRLEDA
                     VQSDPDTEPRSVMVLVEWPSREAFDAFLEDPDHEDLHPLREGGTRNYLWWTYESLEDL
                     RPLFKDARGSSGSRGTQ"
     gene            complement(9268..10455)
                     /locus_tag="VFJ72_14555"
     CDS             complement(9268..10455)
                     /locus_tag="VFJ72_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7480723.1"
                     /translation="MNFDLTDEQRRWRDLARDFAQGEIQPRAAQLDKEQKFPYDLVSE
                     MARLGLMGLTLPEEYGGTGGDFVGYNLALEEISRADTSVGITMEAHISLGCAPLAAYG
                     TPEQKEKFLSKCATGEHLWAFGLTEENAGTDAGGTETTAELRDGEWVVNGSKKWITNG
                     GTDITAGVTAIAKTGTRENGKPELTAIIIPQDTSGFTRGEPYEKTGWRASDQRPLYFE
                     DCRVPEENTLGERGRGFHQFLATLDAGRVAVAALSVGLAQACFDEALARAKEREQFGK
                     SIAEFQAIQFKLADMAMEIELARNMVLKAAWLRDQGRSFGREASMAKVFASETAKRAA
                     DQAVQIWGGEGFMETSAVARYWRQVKINEIGEGTSEINRQIIARSLLSGSTEEVKARM
                     VTQ"
     gene            10593..10862
                     /locus_tag="VFJ72_14560"
     CDS             10593..10862
                     /locus_tag="VFJ72_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7480724.1"
                     /translation="MSIGAIIGWIIIGLIAGALGKLIMPGDDPGGIIVTIILGIAGAF
                     IGGFLASLIGIGGGGVIWTIIIATIGAIILLAIYRAVVGGRTARR"
     gene            11074..12438
                     /locus_tag="VFJ72_14565"
     CDS             11074..12438
                     /locus_tag="VFJ72_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480725.1"
                     /translation="MEKLAGVEIGQQAPMKKIAFASFIGTAIEFYDFYIYGTAAALVF
                     GGVFFPDLSPVAGTLASFATFGVAFLARPLGGALFGHFGDRLGRKAMLIFSLLVMGIA
                     TFLVGLLPGYAAIGIAAPILLVLLRFLQGIGLGGEWGGAVLMAAEHSPPDKRAFYSGF
                     AQVGPPVGFLLSSGLFLLFSSSLSNEQFASWGWRIPFLFSIVLVGVGLFVRATVAETP
                     VFKEVMESRTEAQVPIVDVLRTYPRTVALATLAGSVMFTFFFVITTFSLSYGVTQLRL
                     EQSTMLYCVMVSIALMGAGIMFFATVSDKVGRRNLSLLSTGVLALWAFPMFWLVDTRS
                     PVLITLAFSVSLVAWSAMYGPMGAFFSELFGTRVRYSGASLSYALAGVVGGSLAPYIS
                     ARLLASTGSSWPVSLYLLIAAVVSFVSLLLLSETYRTDLATTRAEERQLIAESQDSNG
                     VRDT"
     gene            complement(12566..12943)
                     /locus_tag="VFJ72_14570"
     CDS             complement(12566..12943)
                     /locus_tag="VFJ72_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480726.1"
                     /translation="MTRKEKVLYHQIHPLKLLTDSTAAFAALPLLWRHRLRAALLVMF
                     VPPVLASIVIIKYVDLEPYKRSSLGRYVKRYMTNEMQAVRLAGALTMALGAWHRRPWF
                     IPFGLLVVLFGWLRGVLFSWRGE"
     gene            complement(13056..13598)
                     /locus_tag="VFJ72_14575"
     CDS             complement(13056..13598)
                     /locus_tag="VFJ72_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7480727.1"
                     /translation="MNRMILLLTVTGTVILLAAGVALAAAINGTNHADRLVGTNAGDT
                     IKGRGGGDSMYGKDGPDVLFGGRGADTMVGSVGNDAMFGGEGNDTLSGGPGLDKLYGG
                     VGDDTIKGGEDNDTTDGQQGSDTLIGGPGNDRIIAGTGDNHHIYARDGEQDLICVDPN
                     SAGTVETSDPNDDFIFNLSC"
     gene            complement(13669..15231)
                     /locus_tag="VFJ72_14580"
     CDS             complement(13669..15231)
                     /locus_tag="VFJ72_14580"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme peroxidase family protein"
                     /protein_id="HET7480728.1"
                     /translation="MSTSRRKDAEERDDEGRISRRNLLFGAATSLIAPILGRPAAASA
                     APAPWTVKRSSASVPGTPRWQHAVGDPRGADIAVAVKRTREARFGLMFKHLPPFLPPD
                     ELLIDLAWEMVDPRPPAPDVSEAADAFDNHDIPAGFTFLGQFIDHDMTRDTSPLSSWQ
                     LDPHGLTNFDTPFFDLGSVYGRGPRLDPQLYDQSTPGKLLLVEHDGVLDLPRAPDGTA
                     FLGDPRNDENLIICQLQIAFIRLHNQFLAAGRSFAEAQRLTRWHFQWVIVHDFLPHVV
                     GQDVVDSLLVQTGSGPIRTKNAFYNPVNPTRPMMPIEYSVAAYRFGHSMIRPEYEAHD
                     GHTRPIFGVEGFDLRGSRPLPPDLKIDWTYFYDIPGLPVPMGRNFARLIDTKLSLPLT
                     HLPPTVIADTRGAITSLAERNLLRGKRLGLPAGQDVARAMGMTPLSNAELGLTDPGWN
                     EKAPLWFYVLKEAELGGGHRLGPIGGRIVAEVILGILALDKTSYFNARTPFSPDTADF
                     KMGDLLHLAGAV"
     gene            complement(15525..>16720)
                     /locus_tag="VFJ72_14585"
     CDS             complement(15525..>16720)
                     /locus_tag="VFJ72_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7480729.1"
                     /translation="TEGLPVLTYEEFLTRAESVSQDELKGRQASLDAEDVINMQYTSG
                     TTGFPKGVQLTHTNIVKNAFLIGECMKLGPEDRVCIPVPFFHCFGCVLGTLNTVTHEA
                     TMVPVEQFDAEKVLQAVDQERCTAVLGVPTMFIAELEHPDFEEYDTSSLRTGIMAGSP
                     CPMEVMKKVVNVMGADEITIAYGQTESSPVITQTRTDDPLELRVSTVGRKLPDTEVRI
                     VSVETGEDCAPGEQGELWTRGYLVMKGYYKMEEKTREVIDEDGWLHTGDLAVMDEDGY
                     VKITGRAKDMIIRGGENVYPREIEEFLYTHPDISDVQVYGVPDEKYGEQVAAAVKVKP
                     GASLTAEEVKDYCRENIAYYKVPEYVDFVEEYPMTASGKIQKYKLRETAVERYNLQTA
                     TADQA"
BASE COUNT         2706 a         5675 c         5155 g         3184 t
ORIGIN      
        1 tcctccatgt tctcgccccg cgcatggtgc gtggcgaact tctcgtcttc cgcccactcg
       61 ggatggccga gcgccttcgc cagccgcccg aagacggtgt cggcgttgcc ggctatcagg
      121 gcgtagtcgc cgtccttcgt cggatagatg ttggaaggcg cgacgaacgg cagcacggtc
      181 ccggtgcggc cgcggacgtg gccggtgagc gcgtactcgg ggatggtgct ctccatgagc
      241 gcgagcaccg cctcgtagat gccgacgtcc acgacctgcc ccttgccgcc gtgagcctcc
      301 cggtggtaga gggcagtgag cgcccccaga gccccgaagg tcgccgcgag cgagtctcca
      361 aggcttatgc cggtgcgcgg cggcggcgcg tcggggaagc cggtgacgtg ccggatgccg
      421 cccatcgcct ctcctatcgc gccgaatccg gcctgctccc ggtacggtcc cgtctgcccg
      481 aagccggaga cccgcaccat taccagtccg gggttgatct cgctgagctt ctcgtacccg
      541 agcccccatc gctccatcgt gccgggccga aagttctcga ctaccacatc cgcctcggcg
      601 attagcttgc gggcgagatc ctggccttcc tcctcgcgca ggttgagcgt cacgctcttc
      661 ttgttgcggg cgatgatggg ccaccagaga gtgcggccct ccttgcgatg ccggccccag
      721 acccgcatcg ggtctccgtt gcccggcggc tcgaccttta tgacctcggc gccgaagtcc
      781 gcgagtagct ggccgcagaa cggaccggcg agcaggctcc ccatctccac tacgcggaca
      841 ccttcgagcg gccgggtgcc gtttcgcgcc tcggtcactg cttctctccc ttccggtcct
      901 cttcgagggc gtggatcacg aagtcccgcc cctcgtagac gtgctcgcgc atgacgatct
      961 cggcccgctc cccgtccccc ttctcaagcg cgcccaggat ctgccggtgg taatggttgg
     1021 agaccatgcg ttcccgcggc gtataccaga agaaggcctt gaacacgagc ggcaactcta
     1081 cggtgcgccg gatcatgcgc tccagccgcc ggttcctgct ggcctcgact atcgttccgt
     1141 ggaactcgtt gttgagcgtg acgagcaacc gtatctcctc ctcccgctcc tcgaagctct
     1201 tctccacgag attttccatc tcgcccgcaa gctcccgcag ccttaccagc ccgcgctcgt
     1261 cgatgcgctc ggcggcccgc cgcgccgcgt ggccttcgag gacggcccgc aggtcgtaga
     1321 tgtcccatac ttcatccacc ccgaacgtgc agaccatcgc tcccttgttc ggctcgatct
     1381 ccacgagacc ctcggcttcg agcatggtga gagcctgccg gataggcgtc cggctcaccc
     1441 ccaacctctc cgcgagctgc tcctcgatca gacgctccct cggaccgtac tcgccttcga
     1501 ggatcatgcg ccgcaaccgc tcgaaaaccg ccgacgatcc cggcctctcc tcacctctac
     1561 gcaaagcggc tcccatggag ttcccttcct ctggaatcac tgctctttcg cagttgttgt
     1621 atacaatctt acgagggagc aagttttgtg tcaagcggtg tatcggcgca cgggtacgct
     1681 actctgaagg gggtccgtca ttccggaccg cgggctcctc acctggtaag gcgaaaggag
     1741 ttttggagag gagaatccta tccccttcta tcagttcacc gtaccggctg acagcccgag
     1801 caccggactc aaggccgaca tcgccaccgc ggtcaccagg gtgcataccg aagtcacggg
     1861 tgccccggcg cgctacgtaa gcgtctcctt ccatgaggtc ccgcccggaa gcattttcgc
     1921 tgccggtgag caggtgtccc aaggacggct ggtaggcatc atccgcagcg gacgcacgca
     1981 ggagatcaag cgcgtactca tcaccgggct ggcaaccgcg tggagtgagg tcaccgggga
     2041 gccgatggaa ggtttcgcgc tctttatcct ggatcggccc ggcagcaaca tcatggatga
     2101 aggtcactcg ctactaccac tcctggtagc gggacgcgcc gtataagttc cggcctcctc
     2161 gaaggagtga gcagccctaa ggatagtctc ctcgtcccac ttcttgccga tcagcatcat
     2221 gccgacgggc aggccgtccg agagggcgca ggggacggtc atcgccgggt gcccggtcac
     2281 gtcgaatggg gcggtattcg ggatcatctc cagggcacgg gtgactatct cctcgcggga
     2341 ggcgtccgcc gacgggatgg gtgtcgcctt catggggagg gttgggatca cgagcaggtc
     2401 caccgactcc agcgcggcgt cataggccgc agcgagcttg cgcccgaggt tctgcgcctt
     2461 ggcgtagtag cggccgtggt agctctcgcg catgtactcg cccaccagca tcgtgagctt
     2521 gacggtctcg gagaggtcgg cggcgcgggt ggtgcgcccc cgggcgtagg cgtccaggag
     2581 ggaggtgctg tagtgcccct tccagttcgt ccccatgctg ttgccgcgca ccataagctc
     2641 ggtggcgccc tctatcgcaa tcccgttcca tatgtggatt ccgtcgcggt gcagcggcac
     2701 ggagacctcg ctcaccttcg ctcccatctc cccgaaggcg cgggccgcct cacgaacggc
     2761 ctcgtcaaca tcatcttccg agaggcccgg ccacccgaaa ccctcggtca ggatgcctac
     2821 tcgcagaccg ctcgcgccgc cgtccaggga accgtaatct ccaacccgga cgtccttctg
     2881 gcgcgggtcg tgaccatcct ctccggctat ggctcccagg aggagcgcga cgtccgagac
     2941 gctggcggct ataggtccgg tgtggtcgag cgtcagctct atcgggaaga cgccggtgta
     3001 cgggacgagc ccgtaggtcg gcttgagacc gtagaccccg caccaggagg aagggatacg
     3061 gatggagccg ccctgatccc cgccgatagc catgtccacc tcgcccgcgg ccactagcgc
     3121 ggcgcttccg ctcgacgaac cgcccgccga gcgtgtgtgg tcgtgcgggt tcaggaccgg
     3181 accggagtcg ctggtgtggc tgccgcccga gaaacagagg tgctcgcaga cagccttacc
     3241 aacgatctcg cctccagcgt ccaggatgcg ggtgacgatg gtggcgtcga actccggcac
     3301 gtatccctcc agcgtggacg tgccgttcat catcgggacc ccggcgacac agacgttgtc
     3361 cttgatcgcc actcgctttc cggacagttg tccatctgcg gcgccttcta tggaacactt
     3421 ctggtaccac gcgccgagag tgttctcctc cggctcgggg cggcggcttc gggagcgcgg
     3481 gtagcggacc ggcagctcgg gctcgactag ctggtccaac ctgcggtaag agccgagcgt
     3541 gccctcgaca agaccgcgaa aagaggtcgc ggcggcatcg tccagtctca gaccaaaacg
     3601 gtccgcgagt tcccgcaagt gctcgttagt cggcggttgc accatgatcc ggactcctct
     3661 cgattgacgg acgcgcccgt acaagagcac gcccgttttc aataaccttt cccgcaagaa
     3721 cggtagcaaa gaccgacttc gcggtcaaag cgatggcgtg cgttctactc gctctccgga
     3781 cgctctatct ccgcgagcac tgctccaccg gggacgagag acccttcgcc gtaggggagc
     3841 gaggtgacgg ttccggcatg gggcgccgtc accggctgct ccatcttcat cgcttccagg
     3901 acgaggagga gctggccctc ctcgacctcg tcgccctcgg acgccacaat ctttacgata
     3961 gttcccggca tcggggcggt cagtccggcg cccgcggaat ctccacccgg tccggcttcg
     4021 tccacgtccg gcggcgcggg acgagcgaga cggtaagtct cgccgccgaa cgagagcgtg
     4081 aggcccgacc tatcggcggc aaactctgct tcggctgact caccgtccac gagcgcccgt
     4141 atccgcccgt cagtgacgtg tagcacctca agggtggatg tctcctcggt caccgttagc
     4201 tttagagtcc ggggaccggc gcgcgcggcc tcgaccgcgc gggtgtctcc gcagacgtcg
     4261 taacgtatct ggagagcgcc catcatgcgc cacggaccca gggtgaaggc atcgccggag
     4321 gtggcggaac gcgagagatc ggccgctgct ccgatgagga gcgcggttcg cggaatatct
     4381 ttctgcgctg gatccgcgag cgcgtgctgg tccagaaagc ctgtcgtagt ctctccggcg
     4441 gcgaaggcgg gatgcccggc gatccgatct agcagcggca ggttggtcgg gacgccgagg
     4501 accgagtagt cgtccagggc gcgccgcagg cgccggacag cggcggagcg gtcgggggcg
     4561 tggacgatca acttggcgag catcgagtcg tagttgatcg gcacctcgtc acccacgtcg
     4621 aagccggtgt cgttacggac gcccgcgccc tgcggcagaa tcagatccag cagcttgccg
     4681 cccacgggca tgcccgcgtc atcttcggcg tagatgcggg cctcgatggc gctgccccgc
     4741 agggtcacat cctcttgcct gaacggcaga gcgtcccccg ccgcaaccgc gagctgaaga
     4801 cgcacgaggt ccagtcccgc aaccatctca gtaacggggt gctcgacctg aagccgcgcg
     4861 ttcatctcca ggaagtagaa gtcctcgccg tccagcagga actctaccgt cccggcgttc
     4921 gagtacccgg cctcgcgggc gagacgcacg gcggcgtcgc agatggtctc gcgcaactcg
     4981 gggctcaggg ccggagatgg cgcctcctcg acgaccttct ggtggcggcg ttggatcgag
     5041 cactcccgtt cgtacagatg cacgacgttg ccatgatcat cgccgattat ctgtacctcg
     5101 acatgccggg ggtcttcgag gagcttctcc aggaataccg atccgtcccc gaaggccgcc
     5161 tccgcctcgc gcctcgcgcc ccgcacggcc tcggcgaact ctttcgggtt cgttacggcc
     5221 cgcatcccgc gcccgccgcc gcccgcgctg gctttcacga ggaccgggta gccgatcctc
     5281 tccgcctcct ccgccaggcg ctcctcggaa tcgtcgcttc cgtcgtatcc ggggacggtg
     5341 ggcacgtcgg cccggcgcgc tatctccttg gccctcacct tgaagcccat cgcctccatc
     5401 gcctccggcg gcggcccgac ccagacggct cccgcctccc ggaccgcccg cgcgaacgcc
     5461 gcgctctcgg agaggaaacc gtatcccgga tgtaccgccg ccgcccccga ccgctcgatg
     5521 gcctcgacga gcctctcgat gttcagatag ctctccggcg ccggagcggg cccgatagca
     5581 taagcctcgt cagccagacg acggtgcatc gcgcgctcgt ccgcctccga gtagacagcg
     5641 acgctccgta tcccgagctc ccggcaggtt cggatgacgc gaacggagat ctcaccccgg
     5701 tttgctacga gcagcttgcc gacggtcacg ggttctctct ccagcggggc tctcgcttct
     5761 ccagaaacgc ccccaggccc tcctgaccct caactccggt ccgaagctcg gagattagag
     5821 gagacatcag agctagagcg tccatcggct ctacagtttc tatctctctg agcagcatct
     5881 tgatggccgc ctgcgcgtac ggcccgccag ccagcagccc atccaccttc ccggagacgg
     5941 cgctgttcag agcatcttca gccaccacct cgtgtaccag cccgatctcc cgcgcccgct
     6001 ccgcgccgaa ccgctctcct gagaggaaca acgagcgggc gtgcgagtac ccgatcttgc
     6061 ggaccacgaa gggcgctatc gtcgccggag ctataccgag ccgcacctcc gagaacgcga
     6121 acctggcacc ttccgcggcc accacgatgt ccgacgcggc cacgagcccc gcgccgcccc
     6181 ccatcgcggc cccgcgaacc ttcgccacca ccggtttcgg acactcgtcc agcgcccgga
     6241 acatcgccgc gaggcgccgg gcatcctcca ggttcttctc gtaggagaga ttcgcagtat
     6301 cgcgcatata ccctatgtca gctcccgcgc agaaagagcg tccctcgccg gtcaggacca
     6361 ccactcgcgc gcgttcgtcg tccgacactt cctcgaagca tgccgtcaac tcctcgatca
     6421 ggggcgcgtt cagcgcgttg cgcgagtcgg gacgcgccag gctgacggtc gttaccggcc
     6481 cttcatcagt ccgccgcagg tgctcgtacg agtttcgata atcggccatc gctacatcct
     6541 gaagatgccg aaggtcgtct cttcgagcgg cgcgttcgcc gcggccgaga ggccgagggc
     6601 caggaccatg cgcgtgtcaa ccgggtctat caccccatcg tcccagagcc tcgccgtcga
     6661 atggtagggg ttcccctcgt cctcgtactt ctccaggatc ggccgccgga actccgtccg
     6721 ttcgtcctcg ttcatctctt ttccctcccg cttgaggttc tcttcctgca cggtcagcag
     6781 cacgttcgcc gcctgaactc cgcccatgac gctgatgcgg gcgttcggcc acatccacag
     6841 aaagcgcgga gagtatgccc tgccgcacat cccgtagttc cccgcgccga aggagccgcc
     6901 cacgatcacc gtgaacttcg ggacgttggc gctggctacg gccatgacca tcttcgcgcc
     6961 gtccttggct atgccgccgg cctcgtactc ctttccgacc atgaagccgg tgatgttctg
     7021 aaggaagacc agcgggatac cgcgctcgca gcaaagctcg atgaagtgag cgcccttctg
     7081 cgcgctctcg gagaacagga tgccgttgtt cgccaggatg ccgaccgggt ggcccatgat
     7141 cctcgcgaac ccgcacacca gcgtctcgcc atacagcggc ttgaactcgt gcaggcggct
     7201 cgcgtcaact atgcgggcta tgacttcacg cgcgtcgtag ccctggcggt agtccgtcgg
     7261 cactatcccg tatagttctt cgatgtcgtg gaggggctct tccggctcct cgatgggcca
     7321 gggcggcttc ttttgcgtgt tcaagttctc tactatggaa cggacgatgg ctaaagcgtg
     7381 ccggtcgtcc tcggcgaaat ggtcggcgac gccggagcgg cgggtgtgga catccgcgcc
     7441 gcccagttcc tctgccgtta cctcctcgcc ggtggcggcc ttcacgaggg gagggccgcc
     7501 caggaagatc gtgccttcgc ccttcactat aactacctcg tcgctcatag ccggcacata
     7561 ggctcctccc gccgtgcagg aacccatcac tgacgctatc tgtgggatgc ccttcgcgct
     7621 catcctggcc tggttgtaga agatgcgacc gaagtgctcc ctgtccggga agacctcgtc
     7681 ctgaagcggt aggaacgcgc cgccggagtc aacgaggtag acgcagggca ggtggttctg
     7741 ctcggcgatc tcctgggccc ggaggtgctt cttgacggtc atagggtagt acgttccgcc
     7801 cttcacggtg gcgtcgttgg cgacgaccat cacttcccgg cccgagacgc gcccgatgcc
     7861 ggtcactatg ccggccgccg ggacccgccc gtcgtacatc ccgtgggccg ccagcggaga
     7921 tagctccagg aaagcggtcc ctggatcgag gagtaggtct atcctctcgc gcaccgggag
     7981 cttgccgcgc tctctgtggc gcttccgcgc cttctccccg ccgccgcgcc gggcctcggc
     8041 ggaccgctcg cgaagctccg atagcacgtt ctcgtaggct ccggcgttgc gccggaagtc
     8101 ctcgcttctg gggtctatgt ggctgcggag tttgctcatc tggcgcccct cgccgtggat
     8161 atcatctcga ccgccatgtt atccggcacc cgcgccagcc gccacgatca ccggccattc
     8221 gagaactgtt gccaaatagt tatcgtttcg acgcccctcc tgtaacctcc gctgcttacc
     8281 atgcgctcat aggataaaga gaacgcgagc ggggaggaga tcaggatgaa gaggacgaca
     8341 ctaacggcgg taactatcgc gctcctgatc tccctctgcg ccagcacggc tctggccgcg
     8401 actgccgcaa cctccggcgc cgacaccctg cgcgggacgc ggagcgtgga tgagcttcgc
     8461 ggcctgggcg ggggcgacac cctgacggcc ctggcgggcg acgacacgct tcggggcgat
     8521 ggcgggcgcg acctcatctc cggcgggggc ggcaacgaca ccatctacgg cggcccgggc
     8581 gacgacgcct cgacccacat cttcgccggt ctcagcggcg gcgcgggcaa cgaccgcgtc
     8641 tacggcggag gcggcaacga ctacataatc ggggacacgg gccgggacct ccttagcggc
     8701 ggggacggcg acgactacat cgccagtacc tccggcgaga gctacgacga acccacctcc
     8761 aaggacaaca tctcctgcgg ccccggattc gataccgtct ctgccgacac ggccgactac
     8821 gtcgcccccg actgcgaaga cgttacgcgc ttctagtaga tcatcaacgg cagcaaccgg
     8881 cgggacggga ggcgcctccc gtcccgctac tgcgtacccc gcgagccgga gcttccgcgc
     8941 gcatccttga agagcgggcg caggtcctcc aggctctcgt aggtccacca gaggtagttc
     9001 cgggtgccgc cctcgcgcag cgggtggagg tcttcgtggt ccgggtcttc gaggaaggcg
     9061 tcgaaggcct cgcgggaggg ccactcgacc agcaccatca ccgagcgcgg ctcggtatcg
     9121 gggtcgctct gcacggcgtc ctccaacctg ccgagcgcca ccacccgccc gccgtgcttg
     9181 cccaccgcct cgacggagcg gcgcgagtac cgacggtata ggtcgttctc cgcgaggtcg
     9241 aagaggttca aggcgtacag gggcatgcta ctgggtgacc atcctggctt tgacctcctc
     9301 ggtggatccc gagaggaggg agcgggcgat gatctggcgg ttgatctcgc tagtcccctc
     9361 cccgatctcg tttatcttta cctgccgcca gtagcgcgcc accgcgctcg tctccatgaa
     9421 cccctcgccg ccccagatct ggaccgcctg gtccgccgct cttttagccg tctcggaagc
     9481 gaacaccttc gccatgctcg cctcccgccc gaacgaacgg ccctgatccc tgagccacgc
     9541 cgccttcagg accatgttgc gcgccagctc tatctccatc gccatgtcag cgagcttgaa
     9601 ctggatcgcc tggaactctg caatagactt cccgaactgc tcgcgctcct tcgcccgcgc
     9661 cagagcctca tcgaaacacg cctgcgccag ccccaccgag agcgccgcca ccgcgaccct
     9721 cccggcgtcg agcgtagcca ggaactgatg gaagccccgg ccccgctcac ccagagtgtt
     9781 ctcctcgggg acccgacaat cctcgaagta gagcggacgc tggtcggagg cgcgccaacc
     9841 ggtcttctcg tagggctcgc cgcgggtaaa gccggaagtg tcctggggga tgatgatcgc
     9901 ggtgagctcc ggcttgccgt tctcgcgcgt cccggtcttc gcgatggccg tgacgcccgc
     9961 ggttatatcg gtgccgccgt tggtgatcca cttcttcgag ccattgacga cccactcgcc
    10021 atcccgtagc tcggcggtcg tctcggtgcc gccggcgtcg gttccggcgt tctcttccgt
    10081 caatccgaac gcccacaggt gttcgccggt ggcgcatttc gagaggaact tctccttctg
    10141 ctccggcgtg ccgtaggcgg cgagtggggc gcagcctaaa gagatatggg cttccatcgt
    10201 aatgccgacg gaggtatcgg cgcgggagat ctcctcaaga gccaggttat aaccgacgaa
    10261 atctccgccc gttccgccgt actcctcggg gagtgttagg cccatcaggc ccaggcgggc
    10321 catctcggag acgagatcgt aggggaactt ctgttcctta tcgagctggg cggcgcgggg
    10381 ttggatctca ccctgcgcga agtcgcgcgc caggtcgcgc cagcgccgct gctcgtcggt
    10441 caggtcgaag ttcatactta tcctcctctt cccggtgatg ccgctgataa tagcagggag
    10501 cggccgccaa aaacgaaaca aatgttcatt acacgacgta taagggaata catggcggcg
    10561 ttagcgatca ccacagaagg aggcagtagg aaatgagcat tggcgcaata atcggctgga
    10621 tcatcattgg cctgatcgcc ggagcgctgg gcaagctgat catgcccgga gacgaccccg
    10681 gcgggatcat cgtcacgata attctaggta ttgccggagc tttcataggc ggcttcctgg
    10741 cgagcctaat cggcatcggc ggcggtggag tcatctggac gatcatcatc gccaccatcg
    10801 gcgcgattat cctgctcgcg atctaccgag cggtagtggg cggtagaacc gcccgccggt
    10861 aaggcaaaac gcaagcgggc cgggatctct cccggccctt tttcgcgtcc gtcctacctg
    10921 ctcgccgtcc cgatccctca gagccgacaa gcaaggtatt gaaccctgtc cgtcccgcgc
    10981 ttaacatgac ccgtatccgg ctaaggtctt cgatggaccg gacaacacta tccggggtgg
    11041 gtctctggct cgcctgcggc aagggaggca ggagtggaga agctcgcggg tgtcgagatc
    11101 gggcagcaag cgccgatgaa gaagatcgcc ttcgccagct tcatcggcac ggcgatagag
    11161 ttctacgact tctacatcta tgggaccgcg gcggcgctag tcttcggcgg ggtgttcttc
    11221 ccggacctgt cgcccgtggc cggtacgctg gcctccttcg cgacgttcgg ggtcgctttc
    11281 ctggcgcgcc cgctgggagg cgcgctcttc gggcacttcg gggaccgcct cggacggaaa
    11341 gccatgttga tcttctccct cctggtcatg ggtatcgcca cgttcctcgt gggcctactg
    11401 cccggctacg cggctatagg tatcgcggcc ccgatactgc tggtcttgct gcgcttcttg
    11461 cagggcattg gtctcggcgg cgagtggggc ggcgctgtac tgatggcggc cgagcactcg
    11521 cccccggata aacgcgcctt ctactcgggc ttcgcccagg tcgggccgcc ggtcggcttc
    11581 cttctctcca gcgggctctt tctactcttc tcctccagcc tgtcgaatga gcagttcgcg
    11641 tcctggggct ggcggatacc cttcctattc agcatagtgc tcgttggggt cgggctcttc
    11701 gtccgggcga cggtcgcgga gacgccggtc ttcaaggagg tcatggagag ccggaccgag
    11761 gcgcaggtcc cgatcgtgga cgtgctgcgg acgtatccgc ggacggtggc gctcgccacg
    11821 ctcgcgggca gcgtcatgtt cacgttcttc ttcgtcataa ccacgttctc gctctcctac
    11881 ggtgtcacgc agctcagact ggagcagtcc accatgctct actgcgtgat ggtctctatc
    11941 gcgctcatgg gagccgggat catgttcttt gccacggtct ccgataaggt cggacgcagg
    12001 aacctgtcgc tcctcagcac cggagtcctg gcgctctggg cgttcccgat gttctggctc
    12061 gtggacacga ggagcccggt attgatcacg ctcgccttct ccgtgagcct cgtcgcgtgg
    12121 tcggcgatgt atggcccgat gggagcgttc ttctccgagt tgttcggcac ccgcgtgcgc
    12181 tactccggcg cctccctcag ctacgccctg gccggggtcg tcggtggttc tctggctccc
    12241 tacatctccg cccgtctatt ggcctcgacc ggaagttcct ggccggtctc cctctacctg
    12301 ctaatcgcgg ccgtggtcag cttcgtctct ctcctgctgc tctcggagac ctaccggacg
    12361 gacctcgcca ccacccgtgc cgaggagcgc cagctcatcg ccgaaagtca ggactcgaac
    12421 ggcgtccggg acacctagcc gccagagtta tgccgtagac agtactttgc gtccgggaag
    12481 ccgccatccg gtcctgtgga ctcacgccgc tcttcctgtg ctgataggcg tcaccaagag
    12541 agcatttcaa gacctgcttc ttgaatcact cgcctctcca cgaaaaaagc actccacgca
    12601 accagccgaa caggacgacg agcagaccaa agggtatgaa ccagggcctt cgatgccagg
    12661 cgccgagtgc catggtcagg gcgcccgcca aacggaccgc ctgcatctcg ttcgtcatgt
    12721 agcgcttgac gtaacgcccg agcgatgatc gcttgtacgg ctctagatcg acgtacttga
    12781 tgatcactat agaggcgaga accggcggga cgaacatcac gagcagcgct gcacggagac
    12841 gatgccgcca gagcagggga agagcagcaa aggcggcggt cgagtccgtg agtagcttga
    12901 gcgggtggat ctgatggtag aggactttct ctttgcgggt catgcgggag acctctgatg
    12961 tgttaggagc gatagctttt gtggctactt cccattgtgc acacgacaca ccaatgacgc
    13021 caagtgtggc ccaccctgag gtcttgaaat actctctaac agctcaggtt gaagatgaaa
    13081 tcatcgttgg ggtccgatgt ctccaccgtg cccgctgaat tcgggtccac acagatcagg
    13141 tcttgctccc cgtcccgggc gtagatgtgg tggttgtcgc cggttccggc aatgatcctg
    13201 tcgttgcccg gaccaccgat gagcgtatcg cttccctgct gaccgtcagt agtatcgttg
    13261 tcctcgccgc cttttatggt gtcgtcgcca acgccgccgt agagcttgtc cagccctgga
    13321 cctccgctca aggtgtcgtt tccttcccca ccgaacatgg cgtcattgcc aacactgcct
    13381 accatggtgt ccgcgcccct gcctccgaac aggacatcgg ggccgtcttt gccgtacatg
    13441 ctatcgccgc ccccacggcc ttttatggta tcgcccgcgt tggtgccgac cagcctgtcg
    13501 gcgtggttgg ttccgttgat cgcggccgcg agcgccactc cggcagccag gaggatcacg
    13561 gtccccgtga ctgttaacag caagatcatc cggttcattt ctggactcct tccgtatcag
    13621 ctacggcgcg cgctgagtaa gtgattggcc actcgagatc cgggctgctc acaccgcgcc
    13681 ggccagatgg agcaggtcgc ccatcttgaa gtcggcggta tctggcgaga atggcgttcg
    13741 ggcgttgaag tacgaggtct tgtccagcgc gagaatcccg aggatcacct cggcgacgat
    13801 ccgtccgccg ataggaccca gccggtggcc gccgcccagc tcggcttcct tgagcacgta
    13861 gaaccacagc ggcgccttct cgttccagcc ggggtcggtg agcccgagct cggcgttgct
    13921 gagcggtgtc attcccatgg cgcgtgcgac gtcctgaccg gctggtagcc cgagccgctt
    13981 gcctcggagc aggttgcgtt cggcgagaga ggtgatcgct ccgcgcgtat ccgcaatgac
    14041 ggtcggaggc aggtgggtca gaggaagcga tagttttgtg tcgataagac gcgcgaagtt
    14101 gcggcccatc ggcaccggca agccggggat gtcgtaaaag taggtccagt cgatcttaag
    14161 gtcgggaggc agcggacggg agccgcgcag gtcgaagccc tccacgccga atatcgggcg
    14221 ggtgtggccg tcatgcgcct cgtactccgg gcggatcatg ctgtgcccga agcggtacgc
    14281 cgcgaccgag tactcgatcg gcatcatcgg ccgggtcggg ttgaccggat tgtagaaggc
    14341 gtttttcgtc cggatcgggc cggagccggt ctgcacgagc aggctgtcca ccacgtcctg
    14401 gccgacgacg tgtggcagga agtcgtggac aatcacccac tggaagtgcc agcgggtgag
    14461 ccgctgcgcc tcggcgaagc tccggccagc ggccagaaac tggttgtgga gccttatgaa
    14521 cgcgatctgc aactggcaga tgatgaggtt ctcgtcgttc cgtggatctc ccaggaacgc
    14581 cgtgccgtcc ggcgcccgcg gcaggtcgag cactccatcg tgctccacga ggagcagctt
    14641 gccgggcgtg ctctggtcgt atagttgggg atccagcctc ggtccccgtc catatacaga
    14701 gccgaggtca aagaacggcg tgtcgaagtt ggtcagcccg tgagggtcga gctgccaact
    14761 ggacagcggc gaggtgtcgc gcgtcatgtc gtgatctatg aactgcccca ggaaggtgaa
    14821 ccccgccggg atgtcgtggt tgtcgaacgc atctgcggcc tccgagacgt ccggcgccgg
    14881 gggccgggga tccaccatct cccaggcgag gtcgatgagc aactcgtccg gcggtaagaa
    14941 cggcgggagg tgcttgaaca ttaggccgaa ccgcgcctct cgggtccgct tcaccgcgac
    15001 cgcgatatcg gcgcccctcg ggtccccgac tgcgtgctgc cagcgcggcg tcccgggaac
    15061 ggaggcgcta cttctcttga ccgtccacgg ggcgggcgcc gcggacgccg cagcgggccg
    15121 gccgaggatc ggagctatca gcgacgtcgc cgctccgaac agaaggttac gacgggagat
    15181 gcgcccctcg tcgtcgcgtt cctcggcatc cttgcgcctg gatgtgctca tttacccggt
    15241 cccttctccc actccggcag ataaaggagc ttgcgtgccg cgcgttcaga ccttttgccg
    15301 tgcatcccgg tccgggcgct ggtaaagacg gctttcgacc gtctgcctat atcccgttgg
    15361 atgagcacaa ggaaccccac tgccgagagt caactgtcaa ctaccggcgc actgtaaccc
    15421 tggcggtggc gctggccatc ggccgtttga ggtatctttg actagctctt tctggctacc
    15481 cgcgattgct ctatctcggg tagcgcggtt gggctagtcc ggggtcacgc ctggtcggcc
    15541 gtggcggtct ggaggttgta gcgctccacc gcggtctccc ggagcttgta cttctggatc
    15601 ttgccgctcg ccgtcatcgg atactcttcc acgaagtcca cgtactccgg caccttgtag
    15661 taggcgatgt tctcccggca gtagtccttg acttcctccg ccgtcaggct cgcccctggt
    15721 ttcaccttca cggcggcggc tacctgctcg ccgtacttct cgtccggcac gccgtagacc
    15781 tgcacatccg agatatccgg gtgggtgtag aggaactcct cgatctccct cgggtacacg
    15841 ttctccccgc cccggatgat catgtccttc gcccgccccg taatcttgac gtagccgtcc
    15901 tcgtccatga cggccaggtc tccggtgtgc agccaaccgt cctcatctat tacttctcgg
    15961 gtcttctcct ccatcttgta gtaacccttc atcaccaggt agccgcgcgt ccataactcg
    16021 ccctgctcgc cgggagcgca gtcctcgccg gtctcgacgc tgacgatgcg gacctccgtg
    16081 tccgggagct tgcgcccgac ggtggagacg cgaagctcca gcgggtcgtc ggtgcgagtc
    16141 tgggtgatga cgggactcga ctcggtctga ccgtaagcta tcgtgatctc atcggccccc
    16201 atcacgttca cgaccttctt catgacctcc atcggacacg ggctaccggc cattatgccg
    16261 gtcctgagag aagaggtgtc gtactcctcg aagtccgggt gctcgagctc cgctatgaac
    16321 atcgtgggca cccccaggac cgccgtacac cgctcctgat ctaccgcctg caacaccttc
    16381 tccgcgtcga actgctccac gggaaccatc gtcgcctcgt gggttacggt atttagcgta
    16441 ccgagcacgc acccgaagca gtggaagaac gggaccggga tgcacaccct gtcctccggg
    16501 ccgagcttca tgcactcccc gataaggaaa gcgttcttta cgatgttggt gtgggtgagc
    16561 tgcacgccct tcgggaagcc cgtcgtcccg gaggtgtact gcatgttgat cacgtcttcg
    16621 gcgtcgagcg atgcctgtcg tcccttcagc tcgtcctgcg atacgctctc ggcgcgggtc
    16681 aggaactcct cataagtcag gacgggcagc ccttccgtgg
//
LOCUS       DASSMB010000255        10244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_976459, whole genome shotgun sequence.
ACCESSION   DASSMB010000255 DASSMB010000000
VERSION     DASSMB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10244
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976459"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..911
                     /locus_tag="VFJ72_14590"
     CDS             111..911
                     /locus_tag="VFJ72_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480730.1"
                     /translation="MNSAIKIPSPRGAFRVWQRNATIFRKYWKSIMFPNFVEPLLYLA
                     ALGLGLGAFIQQGGINGQSYVQYIAPGLLASNAMFAASFESTFNTFVKLKIDRIYDAI
                     ITTPVNAEDVVAGEYLWAGTRAALYGTGFLAMITALGIAFGEPLITSVWAVFIPPMLL
                     VIGMMFSVMGTLFTSLIKRIDLYAYYFTLVVTPLFLFSGIFFPVENFPPPVPTIAWFT
                     PLYHAVNVCRDLATGPSSGTLADVAWILVFTGALALIPVQIMRRRLIS"
     gene            950..1564
                     /locus_tag="VFJ72_14595"
     CDS             950..1564
                     /locus_tag="VFJ72_14595"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7480731.1"
                     /translation="MAEQFTIRRAEPDDDPLIAALVVEGFLDKFRPVFGRRMDHSVKI
                     MQRWVGLEHTSGGVESLVVDWAAEIVGSVGVRTTESREDVLARGLWRTLTRNLGLTRA
                     LWATTLLSYPRYTWTSSEAYVERLVVAPEFRKKGVARGLLHAAEDLARESGKETIGLH
                     VSGQNIPALKLYEDEGYEEVSRQRSLITGYFIGIRDWLYLRKDL"
     gene            complement(1604..3139)
                     /locus_tag="VFJ72_14600"
     CDS             complement(1604..3139)
                     /locus_tag="VFJ72_14600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7480732.1"
                     /translation="MSVIVILVVVFLVVGLVLAASIALGRTAYEVDSARDTEYLDLEG
                     NWIRYNVIGGGPPVVLVHGWLSSSRVWEGLAGRLAQRFTVYTLDLSGFGESDKPLSGY
                     GIRYGSRLLYAFCAHFGLTRTSVIGHDIGGNMAVKLAADHPDVVGRLVLVSTPANEEQ
                     IDLPTPLWLATLPMVGPIFYTLGRLVRGVRRMWLKPFVVHPEDLTEDAVEDAAMSTPS
                     AASKSLNITRREIAGGRLARQARIIKVPVLLVTGEEDQIVDPQAVGVWARSFPQAEVV
                     LMDECGHMPMIERTAEFNAQVLAFLTGDARYLDYVEETRATEDEAFETDEEQEEYAQP
                     VGEPGYEPGYEPADEEIELPPRDDLDRETHPGGEAMYGDDTDAIPTEPVSDESTAGRG
                     DDRRSEPPTIHRKRGGSYSASQSEAGREAGREAERDASAMSFGNGVDGNSQPRDDYRD
                     IFDREPRTRNQGTGKGEIPEVPEDLFSWPSSRREPRARDRSRERSREDEGDFADPETP
                     PER"
     gene            complement(3270..3491)
                     /locus_tag="VFJ72_14605"
     CDS             complement(3270..3491)
                     /locus_tag="VFJ72_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480733.1"
                     /translation="MNDETQKRVITILSSGIAYVLAGRLADKFIKEPEVRGVRDDVKE
                     ALLKAAFSLVSTVIASFIIRRVVGSRWGS"
     gene            complement(3626..3952)
                     /locus_tag="VFJ72_14610"
     CDS             complement(3626..3952)
                     /locus_tag="VFJ72_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480734.1"
                     /translation="MAKEGVETQDIAPGLIVRHTNRRIGILEPCEACERLISGRISFS
                     EFVDQVGDGYRELLDFMMEYDLHPGRPEEVLHCIECYRGPYEGSVLRTQLGYLYDHSR
                     QPEKVC"
     gene            4146..5438
                     /locus_tag="VFJ72_14615"
     CDS             4146..5438
                     /locus_tag="VFJ72_14615"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7480735.1"
                     /translation="MREQTTRRLREAGDGTPRSATLVVVGAGLAGAALIRNLPSSLRR
                     PGATLLIDKNDCYTFAPLLHEVAVGRIHPDSVLSEIPALSRNRCGFLHAGVTSLSPET
                     RTLHTSSGPVSYEYLVLATGSAAASPPEGLVPHLQSFHTLDDALRLRNVLNDAWWTAV
                     EPSNRQDFPGALTVVIVGGGATGVELSAEIAALFGYLRRRSRRSSLTPRIVLVEATDR
                     LMGWLNPYFHDAAMKHLSRLGVEVRLGTFVEEANPDGVRAGEEWIPAAMRLWTAGIEA
                     AGPAARSGMESDESGRVRVDEHLTVLDHPEVYILGDAGIYADPRLGPLPPTGSLAVQQ
                     GPWTARDLGRRLHGSKRRPPFRFSDRGYAVSLGPENAIAEVLGTRIQGPAAQALYRGV
                     LLYFLKNSRDRVLTGADWAMERTIGRLGFERPRERTES"
     gene            complement(5435..5701)
                     /locus_tag="VFJ72_14620"
     CDS             complement(5435..5701)
                     /locus_tag="VFJ72_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET7480736.1"
                     /translation="MTPSQIFVCATPGEGRCGEKGGNELLERFRAEVERRGLSSSAVL
                     RNVCTRRHEEGPVVFVYPDDVWYTRVAASEVPGIVERHLERARV"
     gene            complement(5767..7212)
                     /locus_tag="VFJ72_14625"
     CDS             complement(5767..7212)
                     /locus_tag="VFJ72_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HET7480737.1"
                     /translation="MEAIPSASYSMTLRVEFPHRAGSLGRILTTVGETGGMVGAVDIV
                     RMGEQQTIRDITVNAHDSEHGQQIVRAVEDLPEVRVINVSDRTFLMHLGGKIEVQSKL
                     PIRTRDDLSMVYTPGVARVCRAIHQDPDRAFNLTIKRNTVAVVSDGTAVLGLGDIGPR
                     AAMPVMEGKAALFKQFAGVDAFPICLDTKDTEEIINTVKAIAPAFGGINLEDISAPRC
                     FEIEDRLKRDLDIPVFHDDQHGTAVVVLAALINSLKIVDKRIEDLRIVVNGVGASGVA
                     CAKIMMSAGAKNIVGCDSRGIVHLGREGLNASKQWYARHTNPEGRTGGLSDAIEGADL
                     FLGLSVPDVLTVEHLKSMNEDPIVFAMANPDPEIRPERAYGHAKIIATGRSDYPNQIN
                     NVLCFPGIFRGALDVRAREIDEDMKLAAAQAIANVIPENEVSPDYIIPSVFDERVAPA
                     VAETVAEAAKASGMARRIQERSETDLAATSF"
     gene            complement(7302..7754)
                     /locus_tag="VFJ72_14630"
     CDS             complement(7302..7754)
                     /locus_tag="VFJ72_14630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480738.1"
                     /translation="MNLDELFGGSFVDWEAVATSWSKRSMPSRLLLFAARRYLAEADA
                     PLVPARAQVVEPTPLPDEIKDAFAAPPGPEDPAAARWGEFVDAAVVAELEMISYGERP
                     LVLHELRAGLGQAADEAGARTPLGRWFQARHDALPGTDLPDTPEYLPV"
     gene            complement(7795..9738)
                     /locus_tag="VFJ72_14635"
     CDS             complement(7795..9738)
                     /locus_tag="VFJ72_14635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET7480739.1"
                     /translation="MNSFFQTSKKATAKPRKGFVVRALRALFNLTIILSVTALVLVTG
                     LYLLVVQKYEGQIHQRYPDLAQDSYVYDVNGDRIGEFTAAQSRETVGPDRLGKYLPRA
                     VVAVEDRRFYDHFGVDLEGVARAAWIDARARKVKEGGSTITEQLMKNLFIPEEDRMEV
                     SFWRRFSQSSLAFAYEREHSKKEILTAYLNTVYFGDGAYGAEKAAERYFHKDARQLTL
                     SESAALAGFLHAPSTYASGDVSLAVRRRNEVLDLMRTQGLITKGEYRSARAASLKFAP
                     PPEPEDPAYRPFLEKVRREVQQNMGADTLKEGGLRIYTTLDPDLQREAVVSAEDTLPE
                     TTDPSAAIVTVEPQNGAIRALAGQKDDFNLALDARRQPGSAFKPFVLAAAIHKGISPE
                     STYLSQELALDFGGKSYVIDNYDFIQRGQISLYNAMAESDNTVFVRLARDIGYGNVSA
                     MAENLGIREQVDPYPSTAIGGLAVGVSPLDMASAYATLAGGGIYREPYAVERVDRENF
                     GKSAADYDHRVSGRRVLSGNEAAAVTQVLRGVVKSGTASRFHDLDDETGHPSAGKTGT
                     TDDFVDAWYVGYTPRLSTAVWVGYPEGRRPMVGVHGLSEVNGETLPLDLWSEYMTQAT
                     KEDPVLDFTQPDLSDFTYVQVNN"
     gene            9884..10195
                     /locus_tag="VFJ72_14640"
     CDS             9884..10195
                     /locus_tag="VFJ72_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480740.1"
                     /translation="MPELICTARRIGDYESRTEEYELLFSVGEAPPRELQVRYQAARA
                     YEINAALDSATETARAAISDAHPNLSGKPTDEALAKFAWRAVQSVKYDEGASKTSALE
                     I"
BASE COUNT         1712 a         3421 c         3244 g         1867 t
ORIGIN      
        1 ctaatggaga cggtgcgccg ctccggcatt caggtcgaga acaccgtcta ccggcaatcc
       61 gggctcgaag acgtgttcct gcggctcact ggaaggagcc tgaccgactg atgaatagcg
      121 cgatcaagat tccgtcgccc cgcggagcgt tccgggtgtg gcagcgcaac gcgacgatct
      181 tccgcaagta ctggaagagc atcatgttcc cgaacttcgt ggagccgctg ctgtacctgg
      241 cggcgctcgg cctcgggctc ggcgcgttta tccagcaggg cggcatcaac ggtcagagct
      301 acgtgcagta catagctccg gggctgctcg cgagcaacgc catgttcgcg gcctccttcg
      361 agagcacctt caataccttc gtcaagctca agatagaccg catctacgac gccatcatca
      421 caaccccggt caacgccgag gacgtggtcg ccggagaata tctgtgggcc gggacgaggg
      481 cggcgctcta cgggacgggg ttcctcgcga tgatcacggc gctcggcatc gcgttcgggg
      541 agccgctgat cacctcggta tgggcggtct tcatcccgcc aatgctgctc gtgatcggga
      601 tgatgttcag cgtcatgggg acgttgttca ccagcctgat caaacgcata gacctctacg
      661 cctactattt tacattggtc gtaacaccgc tgttcctgtt ctccgggatc ttcttccccg
      721 tcgagaactt cccgccgccg gtgccaacca tcgcatggtt cacgccgctc taccacgcgg
      781 tgaacgtctg ccgggacctg gctacgggac catcgtccgg cacgctggcg gacgtggcct
      841 ggatactcgt cttcacgggc gcgctggctt tgatcccggt ccagatcatg cgccgccggc
      901 tcatcagcta ggctagttgc tcggggggtc tggtacgatg acatccgaga tggccgaaca
      961 gttcaccata cgccgcgccg agccggacga cgacccactg atcgccgcgc ttgtcgtgga
     1021 ggggttcctg gacaagttcc ggcccgtgtt cggccggcgc atggaccact cggtcaagat
     1081 catgcagagg tgggtcggac tggagcacac ctccggcggc gtagagtctc tcgtagttga
     1141 ctgggcggcg gagatcgtcg gcagcgtcgg cgtccgcact acggagtcgc gggaggacgt
     1201 gctggcccgc ggcctgtggc gaaccctcac gcgcaacctc ggcctgaccc gcgccctgtg
     1261 ggcgacgacc ctgctctcct accctcgata cacctggacc tcatccgagg cttacgtgga
     1321 gcggctagtc gtggctcccg agttccgcaa gaagggggtg gcccgcgggc tcctgcacgc
     1381 cgccgaggac ctggcgcgtg agtccggcaa ggagaccata gggctccacg tcagcggcca
     1441 gaacatcccc gcgctcaagc tgtacgaaga cgaggggtac gaggaagtct cccgccaacg
     1501 ctcgctgatc accggttact ttatcggtat ccgcgactgg ctgtatctaa ggaaagatct
     1561 ctgagcggag caaacgttcc gcccggctga tacgcctggc tacctaacgc tcgggcgggg
     1621 tctccgggtc tgcgaaatcc ccttcgtcct ctcgcgaccg ttcgcgcgag cggtcgcggg
     1681 cgcgcggttc gcggcgcgag gagggccagc taaacaggtc ttctggaacc tccgggatct
     1741 cacctttccc ggtgccctgg ttcctggtgc gcggttcgcg atcgaagatg tcccggtaat
     1801 cgtcgcgtgg ctgtgaattc ccatcgaccc cgttgccgaa gctcatcgcc gacgcgtctc
     1861 gctccgcttc gcgccctgct tcgcgccctg cttcgctctg gctcgccgag taactcccgc
     1921 cccgcttgcg gtggatggtg ggaggctcgg aacggcggtc atcgccgcgt ccggcagtac
     1981 tctcatccga gacaggctcg gttggaatgg cgtccgtatc gtcgccgtac atcgcctccc
     2041 cgccaggatg tgtctcgcgg tcgaggtcgt cccgcggcgg cagctctatc tcttcgtcgg
     2101 caggctcgta gccgggctcg tagccgggct cgccgaccgg ctgcgcgtat tcctcctgtt
     2161 cctcgtccgt ttcgaacgcc tcgtcctcgg tggcgcgcgt ctcttcgacg tagtccaggt
     2221 agcgagcatc gccagtcagg aaggcaagga cctgcgcgtt gaactccgcg gtccgctcta
     2281 tcatcggcat gtggccgcac tcgtccatca acaccacctc ggcctgcggg aagctgcgcg
     2341 cccacacacc gaccgcctgg ggatcaacta tctggtcctc ctcgcccgtt acgagcagca
     2401 ccgggacctt tatgatccgg gcctgccgcg cgagccggcc gccggcgatc tcgcgccgcg
     2461 tgatgttgag agacttgctc gccgccgagg gcgtggacat ggccgcgtcc tcgactgcgt
     2521 cctcggtcag gtcttctggg tgaacgacga acggtttgag ccacatccgg cgcacgccgc
     2581 gcaccagccg cccgagggtg tagaagatcg gcccgaccat cggcagcgtc gcgagccaca
     2641 gaggcgtcgg caggtctatc tgctcctcgt tggccggcgt cgagacgaga acgagacgcc
     2701 cgacgacgtc cgggtggtcc gccgcgagct tcacggccat attaccgccg atgtcgtggc
     2761 cgatgacgct ggtccgggtg agcccgaagt gcgcgcagaa ggcgtacagg aggcggctac
     2821 cgtaacggat gccgtacccg gagagcggtt tgtccgactc cccgaagccg ctgaggtcga
     2881 gcgtatagac cgtaaaccgc tgggcgagcc gtccggcgag accttcccag acccgcgagg
     2941 aagagagcca gccgtgaacg agcactacag gcggcccacc gccaattacg ttgtagcgga
     3001 tccagttgcc ctccagatcc aggtactccg tatcgcgggc gctgtccacc tcgtaggccg
     3061 tgcggcccag ggcgatggac gccgccagga ccagcccgac gacgaggaac acgacgacca
     3121 gaatcacaat cacgctcatg cccgcaatga taacccacta ccgctcgcgg gacacgcggc
     3181 gccagttcgc caatccctcc ggctgcggcg agccttaccc taccttgttc ggtcagaaat
     3241 acagcgccag cgtaagctgt ctagcctctc taggagcccc agcggctgcc caccacgcgc
     3301 cggattatga acgaggctat aaccgtcgag acgagcgaga acgccgcctt gaggagagcc
     3361 tccttcacat cgtcgcgcac gccccggacc tcgggctcct tgatgaactt gtcggcgagc
     3421 cttccggcca acacataggc gatgccgctc gacagtatcg tgataactcg cttctgagtc
     3481 tcgtcgttca tggatgtcct ccctgctatc ggccatcttg acggaccgtt atactctcgc
     3541 ggcccttgcg gattctaccc gctcggcggc ccatgtaacg gagctataac gcggagctat
     3601 aacggaaggg tgccgaacgc gaaggctaac agaccttctc tggctggcgg ctgtggtcgt
     3661 agagatagcc gagctgcgtc cgcaggactg acccctcata cggccctctg taacattcga
     3721 tgcagtggag cacctcttca ggacggccgg gatgcagatc gtactccatc atgaaatcca
     3781 gaagctcgcg gtatccgtct ccgacctgat ccacgaactc cgaaaaagag atgcggcccg
     3841 agatcagccg ctcacacgct tcgcatggtt ccaggatccc aatccgccga ttggtgtgcc
     3901 ttacgatgag cccaggcgca atgtcctggg tctctactcc ctccttcgcc actgcggccc
     3961 ctttctagcc tcgtgagacg attcccaaat tgtcctgtta cccctaagtc tccggccacc
     4021 gccggaggat gcccgtagag tgtacggact tttagacatt ttgtaaagcc tcgcgctaaa
     4081 gaagtttcgc caccccgata tactcgcaga agcgaacgca aaacggtcca aaggagctcc
     4141 ctccgatgcg cgaacagact acccgcaggc tgcgggaagc gggagacgga acgccacgct
     4201 cggcgaccct cgtagtagtt ggcgccgggc ttgcgggggc ggccctgata cgtaacctcc
     4261 cgtcctctct acggcggccg ggcgcgacgc tcctgatcga taagaacgac tgctacacgt
     4321 tcgctcctct cctccacgag gtcgctgtcg gacgcatcca cccggatagc gtcctgtccg
     4381 aaataccagc gctgtcccgg aaccgctgcg gcttcctgca tgccggggta acaagtctct
     4441 caccagagac ccggaccctg cacacgtcct ccggcccggt cagctacgag tatttggtcc
     4501 tcgccacggg gagcgccgcc gccagtcctc ccgagggtct cgtgccgcat cttcaaagct
     4561 tccacaccct cgacgatgcg ctgcgcttgc ggaatgttct caacgacgcg tggtggaccg
     4621 ccgtcgagcc cagcaaccgg caagacttcc ccggcgcgct aacggtagtc atcgtcgggg
     4681 gcggcgccac gggcgtcgag ttgtccgcgg agatagccgc gctcttcggt tacctccggc
     4741 ggcgctcgcg gagatcttcc ttgacgccga ggatcgttct ggtcgaggcg acagaccgcc
     4801 tgatgggatg gctaaatccc tacttccacg acgccgccat gaagcatctc tcccggctcg
     4861 gcgtcgaggt acggctcggc acgttcgtcg aggaggcgaa cccggatggc gtccgggccg
     4921 gcgaggagtg gataccagcc gcaatgcggc tctggaccgc gggcatcgaa gccgcgggcc
     4981 cggccgcgcg ctccggtatg gagagcgatg agtctggaag agtgcgggta gacgagcacc
     5041 taaccgtgct ggatcacccg gaggtctata tcctcggtga tgccgggata tacgcggacc
     5101 cgcggctggg ccccctgcct ccgacgggct ccctggcggt tcagcaggga ccgtggacgg
     5161 ctcgcgacct cggccgaagg ctgcacggct ccaaaaggcg cccaccgttc cgcttctccg
     5221 accggggata cgcggtcagt ctcgggccgg agaacgcgat cgccgaggtg ctgggaacca
     5281 ggatccaggg tcccgccgcc caggcgctgt accggggcgt gctcttgtat ttcctcaaga
     5341 atagccgcga ccgcgtgctc acgggcgcgg actgggcgat ggagcggacc atcgggcgtc
     5401 tcggcttcga gaggccgcgc gaacggacag aatcctagac ccgcgccctc tccaggtgcc
     5461 gctcgactat tccgggcacc tcgcttgccg ccacgcgggt gtaccagacg tcatccgggt
     5521 agacgaaaac caccggccct tcttcgtggc gccgggtaca cacgttgcgc agtacggcgg
     5581 acgaggagag cccgcgccgc tcgacctcgg cccggaacct ctccagcagt tcgttaccgc
     5641 ccttctcacc gcaccggcct tcgcccggcg tggcgcagac gaatatctgg gacggggtca
     5701 tactcgtatt ttattccacg ccggcagcga cggcctctcc agagatcgcg cctcgcctaa
     5761 gctcgcctag aagctggtcg cggcgaggtc cgtctcggag cgttcctgga tgcggcgggc
     5821 cataccgctc gccttggcgg cctcggcgac cgtctcggcg acggcggggg ccacgcgctc
     5881 gtcgaagacg ctcgggatga tgtagtccgg tgagacctca ttctccggga tcacgttagc
     5941 tatagcctga gcggcggcga gcttcatgtc ctcgtcgatc tcgcgggcgc gcacgtccag
     6001 ggcgccccgg aatataccgg ggaagcacag gacgttgttg atctggttcg ggtaatcgct
     6061 gcggccggtc gcgattatct tcgcatgccc gtaggccctc tccggccgga tctccgggtc
     6121 cgggttcgcc atcgcgaaga ctatcgggtc ttcgttcatg ctcttcaggt gctctaccgt
     6181 cagcacgtcc ggcaccgaga gcccgaggaa gaggtccgcc ccctcgatag cgtccgagag
     6241 gccgcccgtg cgcccctccg ggttggtgtg ccgggcgtac cactgcttcg aggcgttaag
     6301 gccctcgcgg cccaggtgga cgatgccccg cgagtcgcag ccgacgatat tcttcgcgcc
     6361 cgcggacatc atgatcttcg cgcacgcaac gccggaggcg cccacgccgt tcaccactat
     6421 ccgcaggtcc tctatccgct tatccacgat cttcagggag tttatgagcg ccgcgagcac
     6481 gacgaccgcc gtgccgtgct ggtcgtcgtg gaagactggg atatccaggt cacgcttcaa
     6541 gcggtcctcg atctcgaagc accgcggcgc cgagatgtcc tccaggttga tcccgccgaa
     6601 ggccggagcg atggccttga ccgtgttgat gatctcctcg gtgtccttgg tatccagaca
     6661 gatcgggaac gcatccaccc cggcgaactg cttgaagaga gccgccttac cctccatcac
     6721 cggcatcgcc gcccgcggcc cgatgtctcc gagccccagg accgccgtgc cgtcggagac
     6781 gaccgccacg gtgttgcgct tgatggtcag gttgaaggcc cggtcggggt cctggtggat
     6841 cgcccggcag acccgcgcca caccgggcgt gtagaccatc gagaggtcat cgcgggtgcg
     6901 tatcgggagc ttggactgga cctcgatctt gccgcccagg tgcatcagga aggtcctgtc
     6961 agagacgttg atgacccgga cctccggcag gtcctcgacc gcgcgcacga tctgctgtcc
     7021 gtgttcggag tcgtgggcgt tgacagttat gtcccggatg gtctgctgct ctcccatccg
     7081 cacgatgtct accgccccta ccatcccgcc ggtctctccg acggtcgtca gaatccttcc
     7141 gagagacccc gcccggtgcg ggaactcgac ccgcaacgtc atgctgtagc tcgcgctcgg
     7201 tatggcttcc atactgtttc tctctccctc tctggagcct gccccacaaa ctcgttgaga
     7261 gagattctat tgcagcctcc agtccgggta aactcgggcc gctagactgg caggtactcc
     7321 ggggtgtccg ggaggtcggt gccgggcagg gcgtcgtggc gggcctggaa ccagcggccc
     7381 agcggcgttc gcgctccagc ctcgtcggcg gcctgcccga gcccggcgcg caactcgtgc
     7441 agcacgagcg ggcgttcacc gtaggagatc atctcaagct cggcgacgac agcggcatcc
     7501 acgaactctc cccagcgggc cgccgccggg tcttcgggac cgggcggcgc agcgaaggcg
     7561 tctttgatct cgtccggcaa cggcgtgggc tcgaccacct gcgcgcgggc cgggacgagc
     7621 ggcgcgtcag cttccgcgag atagcgtcgg gcggcgaaga ggagaagccg ggacggcatg
     7681 ctccgcttgc tccacgaggt agccaccgcc tcccagtcca cgaaagatcc accgaagagt
     7741 tcatccagat tcatggcagc tttataccac gggtccacgg caggcctggc gagtctagtt
     7801 attgacttgg acgtaggtga aatcgctcag gtccggctga gtaaagtcga ggacggggtc
     7861 ttctttcgta gcctgcgtca tgtactcgga ccagaggtcg aggggcagcg tctctccgtt
     7921 tacctcgctc agtccgtgaa cgcccaccat cgggcgccgg ccctcgggat atccgaccca
     7981 gacggccgtg gagagacggg gcgtgtagcc gacgtaccag gcgtccacga agtcgtcggt
     8041 ggtcccggtc ttgccggcgg agggatgccc cgtctcatcg tcgaggtcgt ggaagcgcga
     8101 ggcggtcccg gacttcacga cgccgcgcag cacctgcgtc accgccgccg cctcgttgcc
     8161 ggagagcacg cgccgcccgc tcacgcgatg gtcatagtca gccgcgctct tcccgaagtt
     8221 ttcgcgatcc acgcgctcga cggcgtatgg ctcacggtag atgccgcctc ctgcgagcgt
     8281 ggcgtaggcc gaggccatgt ccagagggct cacgcccacg gcgagccctc cgatggcggt
     8341 cgatgggtaa gggtccacct gctctcttat acctaggttc tccgccattg ctgaaacgtt
     8401 cccgtacccg atatcgcggg cgagacgcac gaagacggtg ttgtccgact ccgccatggc
     8461 gttgtagagg gagatctggc cgcgctggat aaagtcgtag ttgtctatga cgtagctctt
     8521 gcccccgaag tccagcgcaa gctcctgcga gagatacgta gactccggcg agatgccctt
     8581 gtgaatggcc gccgcgagca cgaacggctt gaacgcgctc cccggctgcc gccgggcgtc
     8641 gagggccagg ttgaagtcgt ccttctggcc cgcgagagcc cggatcgccc cgttctgcgg
     8701 ctcgaccgta acgatagccg cggacgggtc ggttgtctcc gggagggtgt cttcggcgga
     8761 cacgacggcc tccctctgca ggtccgggtc cagggtggtg tagatcctga gtccgccttc
     8821 cttcagggta tccgcgccca tgttctgctg gacttcgcgc cgcactttct caaggaacgg
     8881 tctgtaggct gggtcttcgg gctcgggcgg cggcgcgaac ttcagggagg cggcgcgggc
     8941 actgcggtac tctccctttg ttatgagtcc ctgggtccgc atcaggtcca ggacctcgtt
     9001 gcggcgcctc acggccaggc tcacgtctcc ggaggcgtag gtggacggag cgtgcaggaa
     9061 cccggccagg gcggcggact ccgagagggt gagctggcgg gcgtccttgt ggaagtaacg
     9121 ctcggcggcc ttctctgcgc cgtaggcccc atcaccgaag tacacggtgt tcaggtaggc
     9181 ggtcaggatc tccttcttgc tgtgctcccg ctcgtaggcg aaggcgagcg aggactggga
     9241 gaagcgccgc cagaacgaga cctccatccg gtcctcctcc gggatgaaga ggttcttcat
     9301 gagctgctcg gttatggtcg aaccgccctc cttcaccttc ctggcgcgcg cgtcgatcca
     9361 ggccgcccgc gccacgcctt cgaggtccac gccgaagtgg tcgtagaacc tgcgatcctc
     9421 gacggccact accgcgcgcg gcagatactt cccgagcctg tcaggtccga cggtctcccg
     9481 gctctgcgcg gccgtgaact ccccgatcct gtccccgttg acgtcgtaga cgtaggagtc
     9541 ctgagcgagg tccggatagc gctgatggat ctgtccctcg tacttctgca ctacgagcag
     9601 atacagtcca gtaacgagga ccagggcggt cacggagagg attatggtga ggttgaagag
     9661 cgcccggagt gcgcggacga cgaagccctt gcgaggcttt gccgtcgcct tcttgctggt
     9721 ttgaaagaag ctgttcactg atctagcacc tcagtctacc gcaagcggct acacacgata
     9781 cgagcgcacg agataccctg tcactagcgc attccggcgc ttcatatggc gcgggacggt
     9841 tcgctgagga cgcaaccgcg cgggcgcgtg ttaggatgcg cccatgccag agctgatctg
     9901 cacggcccgg cgcatcggcg actacgagag ccgcaccgaa gagtacgagt tgctattctc
     9961 tgttggggag gcgccccctc gggagcttca ggtacggtat caggcggcgc gcgcctacga
    10021 gatcaacgcc gctctcgact cggccaccga gaccgcccga gccgcgatct cggacgcgca
    10081 ccccaacctg tccggcaagc cgaccgacga agccctcgcc aagttcgcct ggcgcgccgt
    10141 ccagtcggta aagtacgacg aaggcgcgtc gaagacctcg gcgttggaga tataagcgtc
    10201 gcggaagtct cagcgccccg gtggtaagcc ggggcgctga gact
//
LOCUS       DASSMB010000256        10560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1346550, whole genome shotgun sequence.
ACCESSION   DASSMB010000256 DASSMB010000000
VERSION     DASSMB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346550"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VFJ72_14645"
     CDS             <1..734
                     /locus_tag="VFJ72_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480741.1"
                     /translation="FGRLIRNAVVLPLLRRDRVQDRLVRKMSQLDMNYRGASLSVDQN
                     ADGLLPRLRRPKTRVRAGDRTPDVVFRDVEAGGRTSLFTLLGRSRPLVLIGSGRTTER
                     TTSITDLVQALDRLGVDCRLVTPHQVGTEPPLADRPLIDVHGDFGRLYGAGDEFLYFI
                     RPDGYVGLFERSIDAQALRGYLKKLFSADDVDDAFSLPDAGRALDPTSQMGAVLVGSR
                     RPEARTASDEQENAMTKKPTLDPDR"
     gene            772..1545
                     /locus_tag="VFJ72_14650"
     CDS             772..1545
                     /locus_tag="VFJ72_14650"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7480742.1"
                     /translation="MNAPTFLVTGGTGSLGRLVSRGLRDAGHDVRVLSHSGRSGTVRG
                     DLLTGEGLDEATSGIDTIVHCASNPRDARHADVEGTGRLLRAAERSDVAHLVYISIVG
                     VDRNPLAYYRAKLDAERVVERSAVPWTILRATQFHHFVLGMLRPLDCVPLFMPVPRGF
                     LFQPVDAGEVADRLVELSLSEPGGRVGDMGGPEVRTAASLAGAYLKAMGRRKRVLEVP
                     LPGKTARAVRNGAQLAPESMWGTLTWKEFLVRTINAKPG"
     gene            1568..2056
                     /locus_tag="VFJ72_14655"
     CDS             1568..2056
                     /locus_tag="VFJ72_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480743.1"
                     /translation="MNTDQDRHGPRRGWLRAGLLILAATVLQAGFWALLFPRAFYDDF
                     PFSGWDWVSTLGAYNGHLVRDYGAMNLAMCFLLVAAIFLERRLSQVALGTLLVFAVPH
                     FAFHLTEIQHFSPFQNAAQFGGLGLQILLPSVLLTLTGRRGDPKTLGHVEHTESCLKV
                     DG"
     gene            2129..3022
                     /locus_tag="VFJ72_14660"
     CDS             2129..3022
                     /locus_tag="VFJ72_14660"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET7480744.1"
                     /translation="MSSAIAGSSGGTRRQARRGLTIYFAVVVMLSASIESFIIKRPDI
                     DGPFIVGLMFVPAIASIVARLVLKEGFSDVSFRFGGRHTWRAIGLALIFPTVVGLTAY
                     GIAWTTGLVRFSSDGSSPIVVLLIGIILATTLWTALSSLVTAGEEIGWRGYMLTRLID
                     SKVSRPVLVSGLIWGLWHVPLVLAEVYAAGPSPALSAAFIMVSIISFGYVIARLRLET
                     GSIWPAIILHSSWNSIIQGPFDGATTGAGAAVWVGESGILTALTLVVVAVVFSRGRWA
                     ILRQPPNPSVAVSLPTSTITG"
     gene            complement(3180..4376)
                     /locus_tag="VFJ72_14665"
     CDS             complement(3180..4376)
                     /locus_tag="VFJ72_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7480745.1"
                     /translation="MNFKLSKRQQEIKERAAELADREVAPHAADLDREDRVPFETLEK
                     MAEAGFMGLCVPEEYGGAGADFLSYCLLLEEISRADAGLGATLAVHTSAGTMPIVVYG
                     TEDQRSRWIPDLARGEKIGCFALTEAEAGSDAAAIKTTAERVDGGYRVNGRKQWITNG
                     RVAGTMVLFARAAGGATEGVTAFIVPMDTEGISFGKHARKMGVISATTDDVILEDVFV
                     PEEDLLGEEGKGLRVALGTLDFGRIGIAAQALGIAEAAFRYATAYAAERTTFGKPIGE
                     HQAIAFKLADMQTKIKAARLLTHEAAWMKDEGLRHTEAGARAKLYASEIANEVAHDAV
                     QILGGQGYMKDHPVERYYRDARVTEIYEGSSEIQRLVISRAILREHAESLEAPGGVED
                     SRTIIG"
     gene            4523..9361
                     /locus_tag="VFJ72_14670"
     CDS             4523..9361
                     /locus_tag="VFJ72_14670"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HET7480746.1"
                     /translation="MLVKDELLDKVLARVREQMPEDQAPQVEEFVRQFYGWITPEDLA
                     GRSSIDVYGAAVSHWTLAQTRETGSTKMRVYNPQFEEHGWQSSHTAVEIVTDDMPFLV
                     DSVRMEINREGYAIHLMIHPIMRVRRDEDGSLVEVLDHEAPDEDAISESVIHVEIDRQ
                     TEPEVLQSIHDNLTRVLGQVRVAVEDWPRMREQAEDIIAGLDEGSIPIDEETVAEVRA
                     FLEWIDDNNFTFLGYREYDLLKQGDEDSLCPVTGSGLGILRETDSKPASHSFAKLPPD
                     VRRLARAPKLLNLTKANTRSTVHRPSYLDYVGVKKFDDAGEVTGERRFLGLYTFTAYS
                     KSAFDIPLVRRKVRYVLERAGFPEGSHNEKDLVEILETYPRDELFQIDREELFDIAMG
                     ILHLQERQRVRLFLRRDTYGRFFSCLVFVPRDRYNTEIRERMQRILQDALSGENVEFN
                     VRLSESVLARLHFIVYTSPERVPTYDVEEVENRLADTVRSWADSLYDALIEQCGEESG
                     VEFYHRYRDAFPPGYRAGFLARTAVNDLRKIERLRSEDDIAMTLYHPLEEPENFLGFK
                     LYRSGEQISLSHVLPILEDMGVEVVDERPHKIEPAGSPPVWIYDFGLVHEAGGELQTG
                     EVKDIFQDAFARTWRGEVEEDGFNRLVLRARLTWREISVLRAYCKYLRQTGVTFSQDY
                     MEDTLFANPHIARLLVELFEARLDPANQGRAERETERLTGEIKAALDEVVSLDEDRIL
                     NSFLNVTLATLRTNYYQSGEDGEPKPYLSFKLDPQKIPELPLPRPRFEIFVYSPRTEG
                     VHLRGGEVARGGIRWSDRREDFRTEILGLMKAQTVKNAVIVPVGAKGGFVVKQPPAQG
                     GREALQNEVVACYKTLIRGMLDLTDNISGDAVVPPRDVVRYDDDDPYLVVAADKGTAT
                     FSDIANGISEEYGFWLGDAFASGGSVGYDHKEMGITARGAWESVARHFRELGLDTQSE
                     DFTVVGIGDMSGDVFGNGMLLSRHIKLVGAFNHLHVFLDPDPDPEASFEERERLFRLP
                     RSSWSDYDQGLISEGGGVFERTAKSIPLSPQVREMLGVEDETMTPTDLISAMLKAEVD
                     LLWNGGIGTYVKASTESNAEVGDRANDVLRVNGDELRCRVVGEGGNLGFTQRGRIEYA
                     LNGGSIYMDAIDNSAGVDCSDHEVNIKILLDAIVEAGDMTTKQRNELLASMTDEVGDL
                     VLRDNYQQTQAINIAKALSHSMADVNSRYIHALEQAGRLNRELEFLPNDETLGERRSE
                     GLGLTAPELAILISYSKISLYKDLLDSDAPEDPYLSRELERYFPTPLRERFREQIHQH
                     RLHREITATHVTNSLVNRCGPTFAYRLADETGAGTPGIAQAYTAARDIFDMRGLWGEI
                     EALDTTVEARIQTGMILDTRKLVERATRWLLRNRRRPFDISAAIAHFSEGAAQLQGLI
                     PDLLLDGDRETLEKSVKKLTEANVPEDLARRVTILGPMFSTLDIVDVAATTGQPLDET
                     AAVYFTLGDRLHLHWLRRHIEKLPRDNRWRTLARSALRDDLYGQQAALTAEILRAIPD
                     QHDAQKRIEAWTEANRGPVDRTIQVLSDINSSSTFDLSTLSVALREIRNLISSSESAS
                     TK"
     gene            9434..9667
                     /locus_tag="VFJ72_14675"
     CDS             9434..9667
                     /locus_tag="VFJ72_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480747.1"
                     /translation="MSFEVVGKITNVETIAVGASIREVQRLRRVYGTGRWRKLKGHAT
                     VRLPDGTVHEAEVHWYEAHGVGKKELKIKYLLD"
     gene            9673..9906
                     /locus_tag="VFJ72_14680"
     CDS             9673..9906
                     /locus_tag="VFJ72_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480748.1"
                     /translation="MDISRAENRFAVCVHNEDYKASLELRKIYRIIPDDTAVGHNLLR
                     VVDESGEDYLYPKHYFIPIELSQEIEEALLAAS"
     gene            complement(9933..>10560)
                     /locus_tag="VFJ72_14685"
     CDS             complement(9933..>10560)
                     /locus_tag="VFJ72_14685"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7480749.1"
                     /translation="GGSTAAARDDFDHRIRPAVDARSEKKGTIYMREAGELLPYGGAS
                     PEVAESAWVAPGAYVIGRVHLGEESSVWYGAVLRGDTEPIRVGARTNIQDGCVLHADP
                     GYPAVVGEGCVIGHKAIIHGCEIGDNCLVGMNATILNGAKIGAGSIVAAGAVVPENRE
                     FPSRSLIVGIPAKRVGEVSEEQAADVERGVRTYVERARAHRASLKVDG"
BASE COUNT         1910 a         3480 c         3300 g         1870 t
ORIGIN      
        1 gcttcggcag gctcatccga aacgccgtcg tcctgccgct gctacgccgc gatagggtgc
       61 aggaccggct cgtgcgcaag atgtctcaac tggatatgaa ctaccgaggc gcgagcctct
      121 ccgtcgacca gaacgcggat ggccttctgc ctcggctgcg gagaccaaag acgcgcgtac
      181 gggccggaga ccggaccccc gacgtcgtct tccgcgacgt ggaagcgggt gggcgaacgt
      241 cactcttcac gctcctcggc cgctctcgtc ctctggtctt gatcgggtcc gggcggacca
      301 ccgaacggac gacgagtatc accgatctgg tgcaggcgct cgaccgtctc ggtgtcgatt
      361 gccgcctcgt gacgcctcat caggtaggta cggagccgcc actggccgac cgccctttga
      421 tcgatgtcca tggagatttc gggcggctct atggcgcggg ggatgagttc ctctacttca
      481 tccggcctga tggttacgtc gggcttttcg agagatctat agacgcacaa gccttgcgcg
      541 ggtacctgaa gaagctgttt tcagcggacg acgtggacga cgcgttcagc ttacccgatg
      601 ccggtcgtgc gctagacccc acaagtcaga tgggagcggt gctggtaggg tcccgccggc
      661 ccgaggcgcg gactgctagc gacgaacagg agaacgcgat gacgaagaaa cccacgctgg
      721 acccggatcg gtgacacaga agcactaact agaggcgaag gaggcttgga gatgaacgcg
      781 ccgacatttc tcgtaacggg aggtacgggt tcgctgggac gtctcgtatc gagaggtctg
      841 cgcgacgccg gacacgatgt tcgtgtcctc agccacagcg gacgttccgg cacggtcagg
      901 ggagatctgt tgaccggtga ggggctcgac gaggccacca gtggtatcga caccatagtc
      961 cattgcgcct cgaacccacg cgacgcccgc cacgcggacg tggaaggtac ggggcgcctg
     1021 ctccgggctg cggagagatc ggatgtggcg cacctcgtgt acatctccat agtcggcgtg
     1081 gaccgaaacc cgcttgccta ctaccgggcg aagctggacg ccgaaagggt ggtagagcgc
     1141 tctgcggtgc cgtggaccat cttgagggcc acccagttcc accatttcgt actcgggatg
     1201 ctccggcccc tggactgcgt gcctctcttt atgccggtac ccagagggtt cctcttccag
     1261 cccgtagacg cgggcgaggt cgccgaccgc ctggtcgagc tctcgctctc ggagcccggc
     1321 ggacgtgtgg gagatatggg tgggccggag gtcaggaccg cagcaagcct ggcgggtgcc
     1381 tacctgaagg ccatgggccg tagaaagagg gtactggaag tgccgctgcc cggcaagacg
     1441 gcgcgagccg tccgcaacgg cgcccagctg gcacccgagt cgatgtgggg cacgctcacc
     1501 tggaaagagt tcctggttcg gacgatcaat gccaagcctg gatagaagag taaggaaagg
     1561 agcgttcatg aacaccgatc aagaccgaca cgggccacgc cgcggctggc tccgggcagg
     1621 tttgctgatc ctggccgcga cggtgcttca ggcgggcttt tgggccctct tgttcccccg
     1681 cgccttctac gacgatttcc ccttctcagg ttgggactgg gtctcgacat tgggcgctta
     1741 caacgggcat ctggtaagag actacggcgc gatgaacctc gctatgtgtt tcctgctggt
     1801 ggcggcgatc ttcctcgaac gtcgtttatc gcaggtagcc ctcggcacct tgctcgtctt
     1861 cgccgtgccg cacttcgcct tccacctcac cgagatacaa cacttctcgc ccttccaaaa
     1921 tgcggcgcag ttcggcggtt tggggttgca gatcctgctc ccctcagtcc tcctcaccct
     1981 taccggaagg cgcggtgacc cgaagacact cggccatgtt gagcacaccg aaagttgcct
     2041 gaaggtggac ggataagggt gcgggagagg tcgccgtcaa tatgtcaagc ccgagtaaca
     2101 taagaactcc tttgaaggga gattggagat gtccagtgca atcgcaggat cgtcaggggg
     2161 cacccggcgc caggcgcgcc gaggactgac catatacttc gccgtcgtcg tgatgctctc
     2221 ggcttcgata gagtccttta ttatcaaaag accggacata gacggcccgt ttatcgtggg
     2281 gctgatgttc gtgcctgcca tcgcctcaat cgtggcgcgg cttgttctta aggaaggctt
     2341 ctcggacgtc tcgttccgct ttggggggcg ccacacctgg agagccatcg ggctggcctt
     2401 gatcttcccg actgtcgtag ggcttaccgc ctacggcatc gcctggacga ccggcttggt
     2461 caggttctcc agtgatggtt catcacccat cgtagtgctc ttgatcggta tcatcctggc
     2521 aactaccctg tggaccgcac tgtcctcact cgttaccgcg ggagaagaga tcggctggcg
     2581 cgggtacatg ctcacgcgcc tcatcgactc caaggtgtcc cggcccgttc tggtgagcgg
     2641 tctgatctgg ggcctctggc acgtgcccct cgtccttgcg gaggtgtacg cggcggggcc
     2701 ttcaccagcc ctctcggccg cgttcatcat ggtctcgatc atatctttcg gctacgtgat
     2761 cgcgcgactt cgactggaga ctggcagcat ttggccagcg atcattttgc actcctcctg
     2821 gaacagcatt atccagggac cattcgatgg ggccacgacg ggcgccgggg ctgccgtgtg
     2881 ggtgggcgag tctgggattc tcaccgcgct cacgctggtc gtagtcgccg tggtcttctc
     2941 ccgggggcga tgggcaatac tccgccaacc gccgaacccg agcgtcgccg tctcgttgcc
     3001 gacgagcacg atcaccgggt gaaactccgg ttcggacaca ccgctcatca gctccccgtg
     3061 cttccgacag cggcgacgct tactttagag ccggcggtgc gaagccggtt cgaaagatac
     3121 aatggctact gatgggtcag ttcgccggac ggtggttgcc ggaagcggga cgcctctggc
     3181 tatccgataa tcgttcttga gtcctcgacg ccgcccggag cttcgaggga ctcggcgtgc
     3241 tcgcgcagga tggcgcggga gatgacgagg cgctgtatct cggaggagcc ttcgtagatc
     3301 tccgttacgc gcgcgtcccg gtagtagcgt tctacgggat ggtctttcat gtagccctgg
     3361 ccgccgagaa tctggaccgc gtcgtgggcg acctcgttgg cgatctcgga ggcgtagagc
     3421 ttggcacgcg ccccggcctc ggtgtggcgc agcccctcgt ccttcatcca ggccgcctcg
     3481 tgggtgagga gacgcgccgc ctttatcttc gtctgcatgt ccgcgagctt gaatgcgatg
     3541 gcctgatgct cgcctatcgg cttgccgaag gtggtgcgtt cggcggcgta ggccgtggcg
     3601 tagcggaaag ccgcttcggc gatgccgagc gcctgggcgg ctatcccgat cctgccgaag
     3661 tccagcgtcc cgagcgccac gcgcaggccc ttcccctcct cgccgagaag gtcttcttcc
     3721 gggacgaaga catcctccag aatcacgtcg tccgtggtcg cggagatgac gcccatcttc
     3781 cgagcgtgct tgccgaagga gatgccctcg gtatccatcg ggactataaa cgctgtaacg
     3841 ccttccgttg ctcctccggc ggcgcgggcg aagaggacca tcgtcccggc gacgcggccg
     3901 ttcgtgatcc actgcttccg tccgttgact cggtatccgc cgtccacccg ttcggccgta
     3961 gtctttatag cggcggcgtc ggagccggcc tcggcttcgg tcagggcgaa gcacccgatc
     4021 ttctctcccc gcgcgagatc cgggatccag cgcgacctct ggtcctccgt gccgtagacg
     4081 actatgggca tcgtgcccgc gctggtgtgg accgccagcg tcgccccgag cccggcatcg
     4141 gcgcggctga tctcctccag cagcagacag taagacagga aatccgcccc cgccccgcca
     4201 tactcctccg ggacgcacag gcccatgaac ccggcctccg ccatcttctc cagcgtctcg
     4261 aacgggaccc ggtcttcgcg gtcgaggtcg gcggcgtgcg gcgcgacctc ccggtcggcg
     4321 agctccgccg cccgctcctt gatctcctgc tgccgcttgc taagtttgaa gttcatgcgg
     4381 tcctccaatg ttccgggttc ctgttcctgc ccccatagta agcgcaacgg ccccaggaat
     4441 gccatcttcg acggcgaacc gggcatgggg ctggtctaca atgatcctac tggaaaggga
     4501 agccgcgtag gagtgtgtgc cgatgctggt gaaggacgag cttctggaca aggtcttggc
     4561 ccgcgtcagg gagcagatgc cggaggatca ggcgccgcag gtcgaggagt tcgtgcgcca
     4621 gttttacggt tggatcacgc cggaggacct ggccggtcgc tcatccatcg acgtctacgg
     4681 ggccgccgtt tcccactgga ccctggctca gactcgtgag acgggctcga caaagatgag
     4741 ggtgtacaac ccgcagttcg aggagcacgg ctggcagtcc tcgcacaccg ccgtcgagat
     4801 cgtcacggac gacatgccct tcctggtgga ctcggtgcgg atggagataa accgcgaggg
     4861 ctacgcgata caccttatga ttcatccgat catgcgcgtg cgccgcgacg aggacggaag
     4921 cctggtcgaa gttctcgatc acgaagctcc cgacgaggac gccatctcgg agtccgtgat
     4981 ccacgtcgag atagaccgcc agacggagcc ggaagtcctc cagagcatcc acgataacct
     5041 cacccgcgtg ctcggacagg tgcgcgtggc cgtcgaggac tggccgagga tgcgcgagca
     5101 ggcagaggac atcatcgccg ggctggacga gggctcgata cccatagacg aggagaccgt
     5161 agccgaggtc cgggctttcc tggagtggat cgacgacaac aacttcacct tcctgggcta
     5221 ccgcgagtac gaccttctga agcaaggcga cgaggactcg ctctgccccg tcacgggctc
     5281 gggcctcggt atcctgcggg agacggactc gaagcccgcc tcgcacagct tcgccaagct
     5341 gccgcccgac gtgcgccggc tcgcccgcgc gcccaagctc ctcaacctca cgaaggccaa
     5401 tacccgctcc accgtccacc ggccctcgta cctggactac gtcggcgtca agaagttcga
     5461 cgacgccgga gaggtaacgg gcgagcggcg cttcctcggc ctctacacgt tcaccgccta
     5521 cagcaagagc gcgttcgaca tcccgctcgt ccggcgcaag gtgcgctacg tgctggagcg
     5581 ggcgggcttc ccggagggca gccacaacga gaaggacctg gtcgagatcc ttgagaccta
     5641 cccccgcgac gagctgttcc agatcgaccg ggaggagctt ttcgacatcg cgatggggat
     5701 tctccacctg caggagcgcc agcgcgtccg gctcttcctg cgccgcgaca cctacgggcg
     5761 cttcttctcg tgcctggtct tcgtacccag ggaccgctac aacacggaga tccgggagcg
     5821 gatgcagcgg atattgcagg acgctctctc cggcgagaac gtggagttca acgtgcggct
     5881 ctcggagtcc gtgctggccc ggctgcactt catcgtgtac acgtcgccgg agcgcgtccc
     5941 gacttacgac gtggaagagg tcgagaaccg cctggccgac acggtacgct catgggcgga
     6001 cagcctctac gacgccctga tcgagcagtg cggcgaggag agcggcgtcg agttctacca
     6061 ccgctaccgc gacgccttcc cccccggata ccgcgctggc ttcctggccc gcacggccgt
     6121 gaacgacctg cggaagatag agaggctccg ttccgaggac gacatcgcca tgaccctcta
     6181 ccacccgcta gaggagccgg agaatttcct gggcttcaag ctctaccggt cgggagagca
     6241 gatctcgctc tcccacgtcc tcccgatcct ggaagacatg ggtgtggagg tcgtggacga
     6301 gcggccgcac aagatagagc ccgcgggctc cccgccggtc tggatctacg acttcggcct
     6361 cgtccacgag gccgggggcg agcttcaaac cggcgaggtg aaggacatct tccaggacgc
     6421 cttcgcccgc acctggcgcg gcgaggtcga agaggacggt ttcaaccgcc tcgtcctgcg
     6481 cgcccggctg acgtggcgag agatctcggt cctcagagcg tactgcaagt acctgcgcca
     6541 gacgggcgtc accttctccc aggactacat ggaggacacc ctcttcgcca acccccacat
     6601 cgcccgcctc ctcgtggagc tattcgaagc ccgcctcgac cccgccaacc agggccgcgc
     6661 cgaacgggag accgagcggc tcaccggcga gataaaggca gccctggacg aggtcgtgag
     6721 cctggacgaa gaccgcatcc tcaacagctt cctgaacgta acgctggcga ccctgcggac
     6781 gaactactac cagagcggcg aggacggaga gcccaagccc tacctctcgt tcaagctgga
     6841 cccgcagaag atcccggagc ttccgctgcc gcgtccccgg ttcgagatct tcgtctactc
     6901 cccccgcacc gagggcgtcc acctgcgcgg cggcgaggtc gcccgcggcg gcatccgctg
     6961 gtccgaccgg cgcgaggact tccgcaccga gatcctcggt ctcatgaagg cccagaccgt
     7021 caagaacgcc gtcatagtcc ccgtcggggc gaagggcggc ttcgtcgtaa aacagccgcc
     7081 cgcgcagggc ggccgggagg cgctacagaa cgaggtagtc gcctgctaca agaccctgat
     7141 ccggggcatg ctcgacctta cggacaacat ctccggcgac gcggtggtcc cgccacgcga
     7201 cgtggtccgc tacgacgatg acgaccccta cctcgtggtc gccgccgaca aggggaccgc
     7261 caccttctcg gacatcgcca acggcatctc cgaggagtac ggcttctggc tcggcgacgc
     7321 cttcgcctcc ggcggctcgg tcggctacga ccacaaggag atgggcataa ccgcccgcgg
     7381 cgcgtgggag tccgtcgccc gccacttccg ggagctcggc ctagacacgc agagcgagga
     7441 cttcaccgtg gtcggtatcg gcgacatgtc cggcgacgtg tttggcaacg ggatgctcct
     7501 gtcccggcac ataaagctcg tcggcgcgtt caaccacctg cacgtattcc tcgacccgga
     7561 ccccgacccg gaggcgagct tcgaggagcg cgagcgcctc ttccgcctcc cgcgctcctc
     7621 gtggagcgac tacgaccagg gcctcatctc cgagggcggc ggcgtcttcg agcggacggc
     7681 caagtccatc ccgctctccc cgcaggtgcg ggagatgctc ggcgtggagg acgagacgat
     7741 gacgccgacg gacttgatca gcgcgatgct caaggccgag gtggacctgc tgtggaacgg
     7801 cggcatcggg acgtacgtaa aggccagcac cgagtcgaac gccgaggtcg gcgaccgggc
     7861 gaacgacgtg ttgcgggtga acggcgacga gcttcggtgc cgggtcgtcg gggagggcgg
     7921 caacctgggc ttcacgcagc gcggaaggat cgagtacgcc ctgaacggcg gcagtatcta
     7981 catggacgcc atagacaact cggccggtgt ggactgctcc gaccacgagg tgaacatcaa
     8041 gatcctgctc gacgccatcg tcgaggccgg ggacatgacg acgaagcagc gcaacgagct
     8101 tctggcgtcc atgaccgacg aggtaggcga cctggtgctg cgcgacaact accagcagac
     8161 gcaggccatc aacatcgcca aagccctgtc gcattcgatg gcggacgtaa actcccgcta
     8221 catccacgcc ctggagcagg ccggcaggct aaaccgcgaa ctcgaatttt tgcccaacga
     8281 cgagactctg ggcgaacggc gctccgaagg tctggggctg accgcccccg agctcgccat
     8341 cctcatctcg tacagcaaga tctcgctgta caaagacctg ctcgactccg acgctccgga
     8401 ggacccgtac ctctccaggg agctggagcg gtacttcccc acgcccctgc gggaacgctt
     8461 ccgcgagcag atccaccagc accggctgca ccgcgagatc accgccaccc acgtcaccaa
     8521 cagcctggtg aacaggtgcg gacctacctt cgcctaccgc ctcgcggacg agaccggggc
     8581 cggaactccc ggcatcgccc aggcttacac ggcggcgcgc gacatcttcg acatgcgcgg
     8641 cctgtgggga gagatcgaag cccttgacac cacagtcgag gcccggatac agaccgggat
     8701 gatcctggat acgcgcaagc tcgtggagcg cgcgacccgc tggctcctcc gcaaccgccg
     8761 ccgaccgttc gacatatccg ccgccatcgc ccacttctcc gagggcgccg cccaactaca
     8821 agggctcatc ccggatctcc tcctagacgg cgaccgcgag accctggaga agtccgtgaa
     8881 gaagctcacc gaagccaacg tccccgaaga cctcgcgcgc cgcgtaacta tcctcggccc
     8941 gatgttctcg actttagaca tagtggacgt agccgccacg accggccagc ccctggacga
     9001 gaccgccgcc gtctacttca cgttgggcga ccggctgcac ctgcactggc tgcgccggca
     9061 catcgagaag ctccccaggg acaaccgctg gcgcaccctc gcccgctccg ccctgcgcga
     9121 cgacctctac ggccagcaag cggccctgac ggccgagatc ctgcgcgcca tccccgatca
     9181 gcacgatgcc cagaaacgca tcgaagcctg gaccgaggcg aaccgcggcc ccgtggaccg
     9241 taccatccag gtgctatcgg atatcaactc cagcagcacc ttcgacctct ccacgctatc
     9301 ggtggcgctg cgcgagatcc ggaacctcat cagttcatcg gagagcgcgt ccacgaaata
     9361 ggcttccaca aaaccaggtg taggatgtgg ttgtttgcca gaaatccgca gcagtgttgg
     9421 tatacggata acgatgagct tcgaggtagt cggcaagatc acgaacgttg agaccatagc
     9481 cgttggtgct tccatccgcg aggtccagcg tctgcggaga gtttatggaa ccggtcggtg
     9541 gcggaagctc aaaggccatg caacagttcg cctgccggat ggcacggtgc acgaagctga
     9601 ggtacattgg tacgaggctc acggcgtcgg caagaaagaa ctgaagataa agtacctttt
     9661 ggattagaga gtatggatat atccagagcg gaaaatcggt tcgcggtttg tgtccacaac
     9721 gaggactaca aagcctcgct ggagttgcga aagatataca ggatcatccc cgacgatact
     9781 gctgtcggac acaatttact gcgcgttgtt gacgagtcgg gagaagatta cctctacccg
     9841 aaacactact tcatccccat cgaactgtca caggagatcg aagaagccct gctggcagca
     9901 tcctgaaaca ccagattcgg gacctcgacg attcagccat ccaccttgag cgatgcccgg
     9961 tgcgctctgg cacgctccac gtatgtcctt acgccgcgct cgacatctgc cgcctgttcc
    10021 tcgctcacct ctccgacgcg ctttgccggg atgccgacta tgaggctgcg ggacgggaac
    10081 tcgcggttct cggggacgac ggccccggcg gctactatgg agccggcccc gatctttgct
    10141 ccgtttaata tcgtggcgtt catgcctacg aggcagttgt cgcctatctc gcaaccgtgg
    10201 atgatggcct tgtggccgat cacgcacccc tcccccacga ccgccgggta gccggggtcg
    10261 gcgtgcagca cacagccgtc ctggatgttg gtgcgcgccc cgacgcggat gggctccgta
    10321 tcaccacgca gcacggcccc gtaccagacg ctcgactcct cgccgagatg caccctgccg
    10381 atgacgtagg ctcccggcgc gacccaggcg gattccgcga cctccgggga ggcgccgccg
    10441 taggggagta gttctccggc ttctctcatg taaatggttc ctttcttctc cgatcgggcg
    10501 tcaaccgcag ggcgtatccg gtggtcgaag tcgtctctag ccgctgccgt tgatccgcca
//
LOCUS       DASSMB010000257         5505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1347050, whole genome shotgun sequence.
ACCESSION   DASSMB010000257 DASSMB010000000
VERSION     DASSMB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347050"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFJ72_14690"
     CDS             complement(<1..1049)
                     /locus_tag="VFJ72_14690"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7480750.1"
                     /translation="MEQRRDSGAGESSDGLRAVWRALGYLRSYKTEAFGAFVALVLAS
                     AASLSTPQLIRIAIDDGIAPRRLNIILLAVGGLVGAAVVRGLFQFLQGYLAERASQSV
                     AYDLRNDLFARIERLGFGYYDRVETGQLVTRLTSDVEQIRTFAGNGVVQLAAAALMLL
                     GTTALLFSLNWQLALVALAIVPVIFVLLLRFVRRIGPLFRSVQQTLGRLNSVLQEDLT
                     GLRVIRTFTREDYETERYRVVNDELYGKNIETVRAFSNNFPFVFLLANLGTLAIILFG
                     GLQVIGGRLTIGELVAFNTYLGFLLFPILTIGFLAAGISRAGASSQRIFEVLDAPLEV
                     RDAPDARPLPPISCRV"
     gene            complement(1055..1471)
                     /locus_tag="VFJ72_14695"
     CDS             complement(1055..1471)
                     /locus_tag="VFJ72_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7480751.1"
                     /translation="MTTENGRAKIPADYKDLLESTALAHVATLGPDGEPQNNPVWFDW
                     DGEHLKFSQTKARQKYRNVGRDPRIALSIVDPENPYRYLEVRGEVVHIEEDPDLDFIN
                     AMAKKYLDQDKYPFHQPGDERVVIHIEPRHTTQMGA"
     gene            complement(1523..2416)
                     /locus_tag="VFJ72_14700"
     CDS             complement(1523..2416)
                     /locus_tag="VFJ72_14700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011566000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7480752.1"
                     /translation="MTDYGRDLEFGVSAEPLADPPDHAAKIVKAADRAGLDLVGIQDH
                     PYQRRFLDTWTLISTLVPVTERVRFFPDVANLPLRPPAMLAKAAASLDVLSGGRVEIG
                     LGAGAFWDAIVAMDGPRRSPAEAVRSVEEAIQVMRLVWSEERSLRFEGEIYALNGMRP
                     GPRPVHDIGIWIGAAGPRMLQLVGRLADGWVPSLFWAPPERLPELHARIDAGAAEAGR
                     QTSEIKRVYNLSGRIGAEGEGLLEGPASTWIEQLTRFVLECGMDTFIYWPSEDHERQV
                     EAFANEVVPAVREAVAKERRG"
     gene            complement(2413..3831)
                     /locus_tag="VFJ72_14705"
     CDS             complement(2413..3831)
                     /locus_tag="VFJ72_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480753.1"
                     /translation="MSKEQTATGGKVSTRLLLGVLISAVFVSVLNSSMVNVVVPVIGR
                     DYGASEAQVGWVITGYLLTYAVGIPLYGRVADIFSIRRAFAVGLLAFAVGSLLCAWAP
                     SLGTLVFGRIVQGAGGAAIPALSSVSIAKVLPPGERGSALGLIVSSVGIGASVGPIVG
                     GAVAALAGWHYLFIGTLLLTLALLPAALYVLPDDSPEGSRGFDLPGGVLLGLAAGLFL
                     YGITRGQVSGFGSASSWGSFIGAALAAAGFAWRIRSAAEPFVSPALFKNRAYVAAVAV
                     GYFSMLANVSALVFVPLLVSQVNGLSPGQAGLVLTPGAVALAVLSPVAGRLSDRIGAR
                     VLILVGLGIMLLSILSISTFGAGASPVIVALGMLGVGTGFALANSPNVNAAASVLEPD
                     EVGAGLGIFNGLFFLGGGTGPAVIGAFLAARKEADAGALNPLYGLDAAPYSDAFLVLI
                     VALLISLVATLGLRGNTGKEKK"
     gene            complement(3828..4403)
                     /locus_tag="VFJ72_14710"
     CDS             complement(3828..4403)
                     /locus_tag="VFJ72_14710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET7480754.1"
                     /translation="MGGTLRKGSTSLGALRQALASAEEAGAEVELLDLRELRLPMYEP
                     GLALDEYGPEVRRFVEEIQDADALIVSTGAYHGTLAGVTKNALDFMQFLSRDERPYLD
                     GRVVGLISTAGGEQAAANSNAAMVHAVHALRGIVAPLMVAIPKAWQRSEDGEITDDTY
                     AARLDQLGALVVDLSGRLQRPDEARIAGAVA"
     gene            complement(4507..4995)
                     /locus_tag="VFJ72_14715"
     CDS             complement(4507..4995)
                     /locus_tag="VFJ72_14715"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7480755.1"
                     /translation="MRGTARTEKGIQTHPVKRLTGFTLAKTCKAHRGNVGGMLSRHGL
                     HVGQEMVLMELWQEDGLRGGELAVRLGVEPPTITKMLRRLEGCGMVERRPDPTDARSF
                     RVHLTSEGRALEEPVARCWETAEERLLRGMSAEERRVFHELLVRARSNLERGSGAAIT
                     TH"
     gene            complement(4992..5393)
                     /locus_tag="VFJ72_14720"
     CDS             complement(4992..5393)
                     /locus_tag="VFJ72_14720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7480756.1"
                     /translation="MSSFTKAEISYMREQRLGRLATVDAAGVPHVVPVGFRYNEEMDT
                     IDIGGRNLGRTKKFRDARTTGKAAFVVDDVPPPWKARGVEVRGRAEVYSEGGGEIVEG
                     FSDEVIRIHPQRIVGWGLDSDAFRPNSRSVG"
BASE COUNT          917 a         2004 c         1724 g          860 t
ORIGIN      
        1 acccggcacg agataggggg gagaggcctg gcgtccggcg cgtcccgcac ctccagcggc
       61 gcgtccagga cctcgaagat gcgctgcgac gaggccccgg cccgcgagat gcccgcagcc
      121 agaaaaccta tcgtcaggat cgggaacagc aggaagccca ggtacgtgtt gaacgcgacc
      181 agttccccga tggtgagtct gcccccgatc acctgcaacc cgccgaacag gatgatcgcc
      241 agcgtcccca ggttggcgag caggaacacg aacgggaagt tgttggagaa cgcgcgcacg
      301 gtctcgatgt tcttgccgta aagctcgtcg ttgaccacgc ggtagcgctc cgtctcgtag
      361 tcctcgcggg tgaaggtgcg gatgacacgc agtcccgtca ggtcttcctg aagcacggag
      421 ttcaagcgtc cgagcgtctg ctgcacgctg cggaagagtg gtccgatcct gcggacgaag
      481 cgcaggagca gcacgaagat caccggcact atggcgagcg ccacgagcgc gagctgccag
      541 ttgagcgaga acagcagggc ggtcgtgccc agcagcatca gtgcggcggc ggcgagttgc
      601 acgacgccgt taccggcgaa cgtccggatc tgctccacgt cgctcgtcag ccgcgtcacg
      661 agctggcccg tctcgacccg gtcgtagtag ccgaacccta accgttcgat cctcgcgaac
      721 aggtcgttcc gcaggtcgta ggcaacgctc tgggaagccc gctccgccag atacccctgc
      781 aagaactgga acaacccgcg caccaccgcc gccccgacca gcccgccgac cgccagcagg
      841 attatgttca aacgacgcgg cgcgatgccg tcgtctatgg cgatcctgat gagctgcggt
      901 gtagacaggc tcgccgccga cgcgagcacc agcgcgacga aggccccgaa agcctcggtc
      961 ttgtacgagc gcaggtagcc cagagccctc cagacggcgc gcaggccgtc cgaggactct
     1021 ccggcgccgc tgtcccggcg ctgctccaaa cttgctatgc gcccatctgg gttgtgtggc
     1081 gcggctcgat gtggatgacc acccgctcgt cgccgggctg gtggaagggg tacttgtcct
     1141 gatccaaata tttcttcgcc atcgcgttga tgaagtccag gtccgggtct tcctcgatgt
     1201 ggacgacctc gcctcgtacc tccaggtagc ggtaggggtt ctccgggtcc acgatggaga
     1261 gggcgatgcg ggggtcgcgg cccacgttgc ggtacttctg gcgggccttc gtctggctga
     1321 acttgaggtg ctcgccgtcc cagtcgaacc agacggggtt gttctgcggc tctccgtcgg
     1381 ggccgagggt ggcgacgtgc gccagcgccg tgctctccag caggtccttg tagtccgcgg
     1441 ggatcttcgc tctaccgttc tccgtggtca tccgtgtcct ttcgttgtcg ggttgctgtc
     1501 gccaaagagt ttatcctctc atctaaccgc ggcgctcctt cgccacggcc tcgcgcaccg
     1561 ccgggacgac ttcgttcgcg aacgcctcta cctggcgctc gtggtcctcc gagggccagt
     1621 agataaacgt gtccatcccg cactccagaa cgaaccgggt tagctgttcg atccaggtcg
     1681 aggccggtcc ttccagtaga ccttcgccct cggccccgat cctgccggag aggttgtaga
     1741 cgcgctttat ctccgaggtc tgcctgcccg cttccgcggc cccggcgtct attcgggcgt
     1801 gcagctccgg caggcgctcc ggcggagccc agaacagcga cgggacccag ccgtccgcga
     1861 gccgcccgac gagttgcagc atccgggggc cggccgcccc gatccagatg ccgatgtcgt
     1921 ggacgggccg cggtccgggc cgcatgccgt tgagcgcgta gatctcaccc tcgaaccgca
     1981 gtgagcgctc ctcgctccac acgagccgca tgacctgtat cgcctcctcc acggaacgga
     2041 ccgcctccgc cgggctcctg cgcgggccgt ccatcgccac gatcgcgtcc cagaaagctc
     2101 ccgcgccgag cccgatctcg acccgcccgc cggagagcac gtcgaggctc gccgccgcct
     2161 tcgccagcat cgcgggggga cgcagcggca ggttcgccac gtccgggaag aaccggacgc
     2221 gctccgttac cgggacgagc gtggagatga gcgtccacgt atccaggaag cgccgctggt
     2281 acgggtggtc ctggatgccg acgaggtcga gacccgcccg gtccgccgcc ttcactatct
     2341 tcgccgcgtg gtccggcggg tccgccagcg gctccgcgct gacgccgaac tccaggtccc
     2401 tgccgtagtc ggtcacttct tctcctttcc ggtgttgccc cgcagtccca gcgtcgccac
     2461 cagcgagatc aggagcgcca cgatcaggac caggaaagcg tccgagtacg gcgcggcgtc
     2521 cagcccgtag agggggttga gcgctccggc gtccgcctcc ttgcgggccg ccaggaacgc
     2581 gccgatcacc gccggacccg taccgccgcc gagaaagaac aggccgttga agataccgag
     2641 ccccgccccg acctcgtccg gctccaggac cgaggcggcg gcgttgacgt tcggggagtt
     2701 tgcgagggcg aagccggtcc cgacgccgag catccccaac gccacgatca ccggcgacgc
     2761 cccggccccg aaggtcgaga tagagaggat tgagagcagc atgatcccca gccccacgag
     2821 aatgaggacg cgcgccccga tcctgtccga caaccttccc gccaccggcg acagtaccgc
     2881 cagcgccacc gcgcccggcg tgagtaccag ccccgcctga cccggagaca ggccgttgac
     2941 ctgcgagacg agcagcggca cgaacaccag cgccgacacg ttcgccagca tggagaagta
     3001 cccgaccgcc acagccgcca cataggcccg gttcttgaac agcgcggggg agacaaacgg
     3061 ctccgcggcg ctacgtatgc gccaagcgaa tcccgcggcc gccagagctg cgccgatgaa
     3121 gctgccccac gaggacgctg acccaaaacc ggacacctgt ccgcgagtta tcccgtacag
     3181 gaagagtccc gccgccagtc cgagcaggac gccgccgggt aggtcgaagc cgcgcgagcc
     3241 ttccgggctg tcgtccggga ggacgtagag ggcggcgggg aggagggcga gggtcaggag
     3301 caaggtgccg atgaacaggt agtgccagcc ggcgagggcc gccaccgcgc cgccgactat
     3361 agggccgacg gacgccccga tgccgacgct agagacgatc aggccgagcg ccgatccccg
     3421 ctctccgggc ggcagcacct tcgctatgga gacgctggag agggcgggga tggccgcgcc
     3481 gcccgcgccc tgcacgatac gtccgaatac cagcgtcccg aggctcggcg cccacgcgca
     3541 caacaagctc cccacggcga acgccaggag gccgaccgcg aaggcgcgcc ggatgctgaa
     3601 gatgtcggcg acgcggccgt agagggggat gccgacggcg taggtgagga ggtagcccgt
     3661 gatcacccac ccaacctgcg cctccgaagc gccgtagtcc cggcctatga ccggcacgac
     3721 cacgttcacc atagagctgt tcaggaccga gacgaagacc gccgagatca ggaccccgag
     3781 caggagccgc gtggagacct ttcctcccgt cgcggtttgc tccttgctca agcgacggcc
     3841 ccggctatcc gcgcctcgtc cggtcgttgc agcctgccgg acaggtccac gacgagcgcg
     3901 ccgagctggt ccagccgcgc ggcgtaggtg tcgtccgtga tctcaccgtc ctccgaacgc
     3961 tgccacgcct tcgggatcgc caccatcagc ggcgccacga tgccgcgcag cgcgtgaacg
     4021 gcatggacca tcgcggcgtt agagttggcc gccgcctgct cgccgcccgc cgtggagatc
     4081 agtccgacca cccggccgtc caggtatggc cgctcatccc gcgagaggaa ctgcataaag
     4141 tccagcgcgt tcttcgtaac gcccgcgagc gtcccgtggt aggcgcccgt gctgactatc
     4201 agggcgtcgg cgtcctggat ctcctcgacg aaacgccgga cctccggacc gtactcgtcg
     4261 agagccagcc ccggctcgta catcgggagc cgcaactccc gcaggtccag aagctccacc
     4321 tccgccccgg cttcctctgc gctcgccagc gcctgcctga gcgccccgag gctggtcgaa
     4381 cccttccgca gcgtgccacc caatccaact accttcaaat ggtctctcaa aacgtacccc
     4441 tctcaaactc tcaaaataag agtcttaatg ccggactaat tgtaagccgc ccggctcctt
     4501 gtgttgtcag tgcgtggtta ttgccgcgcc ggagcctcgc tccaggttgg accgcgctct
     4561 gaccagaagc tcgtgaaaca cgcgccgctc ctccgcgctc ataccccgca gaagccgctc
     4621 ctcggccgtc tcccagcaac gggcgacggg ctcttcgagg gcacgtcctt ccgatgtgag
     4681 gtgtacccgg aagctccggg cgtccgtggg gtccggcctg cgctcgacca taccgcaccc
     4741 ctccagccgg cgcagcatct tggtgatggt cggcggctcg acgccgagcc ggaccgcgag
     4801 ttctccgccg cgcagcccgt cctcctgcca cagctccata agcaccatct cctgcccgac
     4861 gtgcaggccg tgccgggaca gcatccctcc gacatttccc cggtgcgcct tgcaggtctt
     4921 cgccagcgta aagccggtca atcgctttac ggggtgagtc tgtatcccct tctccgttct
     4981 cgcggtgccc ctcaaccgac ggacctgctg ttcggacgga aggcgtcgga gtcgagaccc
     5041 cacccgacga tgcgctgggg gtggatccgg atcacctcgt ccgagaagcc ctcgacgatc
     5101 tcaccaccgc cctccgaata gacctccgcc cggccgcgca cctccacgcc ccgcgccttc
     5161 cacggcggcg gcacgtcgtc caccacgaac gccgcctttc cggtggtcct cgcgtcccgg
     5221 aacttcttcg tcctgcccag gttacgcccg ccgatgtcta tggtgtccat ctcctcgttg
     5281 tacctgaagc ccacgggtac cacgtgcgga acgcccgccg cgtccaccgt ggcgagccgc
     5341 cccagacgct gctcccgcat gtaagagatc tccgccttcg taaagctgct catgcccgct
     5401 ccgacttcct gcgcgccgtc agccgctcgt agttgagccg gaacagctcg accgctcctt
     5461 ccacgtcctc ctcgccccgg atgcggtagc tcacccaccc ggtct
//
LOCUS       DASSMB010000258         8808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_207974, whole genome shotgun sequence.
ACCESSION   DASSMB010000258 DASSMB010000000
VERSION     DASSMB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207974"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VFJ72_14725"
     CDS             complement(<1..321)
                     /locus_tag="VFJ72_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7480757.1"
                     /translation="MDLGGRVALVTGASRGIGAAIAERLSGVGADVAVGYGNDEESAG
                     KVVARVEPEGRRAVAVGGDLRDPAAVEEMCSAVESSLGPVDVLVSNAGIAPKQSFEEI
                     TDSDW"
     gene            complement(390..1376)
                     /locus_tag="VFJ72_14730"
     CDS             complement(390..1376)
                     /locus_tag="VFJ72_14730"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7480758.1"
                     /translation="MKQRTLGSQGLVVSELGLGCMGMSEFYGTPDEDESIATIHRALE
                     LGINFLDTADMYGPFTNEKLVGKAIADRRDDVVLATKFGNERGEDGARLGINGRPEYV
                     RRACDDSLKRLGIDYIDLYYQHRVDPDTPVEETWGAMKELVEAGKVRYLGISEAAPDT
                     IRKAHAVHPVTALQTEYSLWSRDPEDEILPTVRELGIGFVAYSPLGRGFLTGQFEKFE
                     DLPEGDYRRSSPRFQGENFEKNLQMVERVREIAEEKGATPGQLALAWLLAQGDDIVPI
                     PGTKRRKYLEENAAAVDIELTDEDLARIEEAAPVGSAAGERYSEESMKSVNV"
     gene            1744..3222
                     /locus_tag="VFJ72_14735"
     CDS             1744..3222
                     /locus_tag="VFJ72_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HET7480759.1"
                     /translation="MKNSRHSKPGAYNVAGLAWTRLRIGPIDLALVATTAVMLATMVS
                     AAKLTIGCFHVVFVLLTFGAFFWKFRAFVWRAVFWVAVDTGVVLLAAVYGSLPYDELI
                     EIPLLSIILVLVFMIARRRAEAQARLQEQDEAFLEAILGNMEDAVVACDAGGRLAFSN
                     PAARTIHGLPEGLPEGLPEELPLEQWTDHYELRTETGATVDEADIPILRALRGERIKD
                     EEFVISTKDNPARYLLVSGNRMQGSLEQTLGAVVVMRDITERKLAERRLREVQEDERE
                     RIARDLHDAVLQDLSWAMRKIEYDRMMGLVDLKNDLASEQVVPALRRAVQGVRNSIYD
                     LFPRDASKLPFAELLESALDLNREISPEIDFEAVIGEFPRKLPQPAGAEVMKIIQEAL
                     VNARRHSSASRVRVTLDIEGDQLVVCVEDDGRGFEPHTVPGLGMAGMRERAERLGGSL
                     EIHSRELEGTRVCFSAAKERLTHPQQPQEKLSWVSGKQPGSS"
     gene            3321..3992
                     /locus_tag="VFJ72_14740"
     CDS             3321..3992
                     /locus_tag="VFJ72_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480760.1"
                     /translation="MAEEPIESVRIMVVEDHSSFRQALAHMLDLEPGFSVVAEAGTLA
                     EARKRSKEKPDVAIVDMALPDGEGTELIRELHKSGDRVTALVLSGSLSRRQYARAVEA
                     GAAGVMHKTSSIEEIVDAVRRLKAGESLLSLDEVVALFRDVGQEREQNRDAKQAVESL
                     SQREREVLSALAEGLDSREIAERLNITVDTERSHFIRIFAKLGVHSRLQALVFAVRHG
                     IVEIH"
     gene            4100..4441
                     /locus_tag="VFJ72_14745"
     CDS             4100..4441
                     /locus_tag="VFJ72_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480761.1"
                     /translation="MRKTRLLLVNEPLSYRDAIAHVFRELRPNLEVLACDPDDVESGL
                     EALAPDVVICSALTPAIRDGVRVWIELYPGGEPLAVVSIAGERATFSDIELPDLLSVV
                     DSTTEILQHNL"
     gene            4744..5115
                     /locus_tag="VFJ72_14750"
     CDS             4744..5115
                     /locus_tag="VFJ72_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480762.1"
                     /translation="MSLVASLLHLLVVPEHFAEWWGYGAFFLITALAQGIGGVALLRW
                     PMRPLFLAGIAGNGAIVAMWLASRTVGIPFFGPHAWEVEGVGTIDLACTISEVALIAA
                     LGALARSKRRHQRETSGAYAT"
     gene            5195..>8808
                     /locus_tag="VFJ72_14755"
     CDS             5195..>8808
                     /locus_tag="VFJ72_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480763.1"
                     /translation="MTPAKTLKVRRWDRGARLLAALLLLLAAALLVSQSPALVQQAGA
                     QTSGPSSLVVKDLKTGDPIANFNYTINADNVGDPADPANMPSVIKGQSNSPVAAVGDQ
                     GSAGSVDLDPGKYVASVSADGYKLGGEHFSAGDTVTVLLEPEPKAGLDPSRLAKIRVH
                     AFEDNRMVNGENDAPLESGLEGFDVVIKDEVGHVTQDYYGNAIGTEYEKDANGDLVLD
                     PNGQPIPVPNTGGNILTDANGDAVVENLAPGKYGVQVIAPDGSGWIQTSTLEGTHTID
                     AWIDEGNDGTISEVPGVSPSPMAEIGFVKEQSFANPGSGSIKGRARTALEYITEAANP
                     PLGDPVKSPYIALTALDLNDELSYLGRGNPDGTFDIQNVPDGKYQLTIWDEPMDYIIS
                     FRTATVSGGNVVDMGDIGVPRWFGKTLGTVFEDNGKDGNGNPITRADGTDAAGNQILD
                     CDQNDPNSCEKGLPGVDVGTRYKDGSTQYGTLTDAQGHYALNETFPLGRFTVAEVSNV
                     RYAPTGASVRSEYNGTDLKSLSVPGALLMSTFSWTGKNYIDWGKKRYTGSENGGISGI
                     VYYATMRTEENARYQSGDPYEPGVPGVKVDLYQADTDANGDPIKEADGSVKTKTADPI
                     ATVNTDSWQQPTGCDVLDENGNTPTDPAYAPTAQQIADNCLEVPALGNETRDGVFDGG
                     YAFSTYTDPSDGQTKPLPPGDYVVKMRIPKDKNGNDLYKIVKEEDVNIEKGENFVPQV
                     PPPPCAGALHTVNDPRQGPYDGQQMPLCDSKLVHLNAQQNAGADFFMFTDTPIPGRVW
                     GLVTDDLNIETDPSLLYYGDKKGIAHIPVGVYDHTGRLIQTVYTDDNGIFETILPSTG
                     TFNVPTPSGVSPNMYTFLANDPGPDPNNPNPVQGPGTVMGYDPNYSTLPLIYDVWPGK
                     TTAADMAILPNTPGGDPGGAQAGEPEDGAPDVQNVQNPVVQAAGARNLVIKGKDFGAA
                     PQVTLDGKQITVNSMSGDTQINAAVPAGFPVGPHELLVKSGGRTSATGLKVHVLGTSG
                     KTTYRPTVIRVNQGQKIQDAINRASGNTLIVVGPGTYNENLIVHKGVKLQGVGPGSVT
                     INGSYVDAHSAAWHSLLGSVNFDGDRNVNEGSAITVLAKAGQFTPTFRPSIDGFTIKG
                     GRGDSGGGIFVNGYGRYLDISNNVIQGNRGYYGGAVTLGRPYSGDAHNTNVRIRYNRV
                     L"
BASE COUNT         1697 a         2943 c         2750 g         1418 t
ORIGIN      
        1 ccagtcagag tccgttatct cctcaaagct ctgcttcggc gcgatcccgg cgttgctcac
       61 caatacgtca accggaccta aagagctctc gaccgcgcta cacatctcct ccaccgccgc
      121 cgggtctctc aaatctcccc caacggccac cgccctacgg ccttcaggct ccacgcgcgc
      181 gacgaccttg cccgccgact cctcgtcgtt cccgtagccg acagccacgt ccgcgccgac
      241 cccggagagc cgttccgcta tcgccgcccc gatcccgcgg ctcgctcccg ttaccagcgc
      301 gacgcgccct ccaagatcca tgtctacccc cgtagagatc ggggcgagcc ttgtggcccg
      361 ccccggtaaa gacgcttcct taccgcccgt tatacgttga cggacttcat gctctcctcg
      421 ctgtagcgtt cgcccgcagc cgacccgaca ggcgcggcct cttctatgcg agccagatcc
      481 tcatcggtga gttcgatgtc cactgcggcg gcgttctctt caaggtactt gcgacgcttg
      541 gtgccgggga tcgggacgat atcgtcaccc tgagccagga gccaggcgag cgcgagttgg
      601 ccgggcgtag ctcccttttc ttccgctatc tcccgcaccc gctcgaccat ctggaggttc
      661 ttctcgaaat tctcgccctg gaagcgcggg gagcttcggc ggtagtcgcc ctccggcagg
      721 tcctcgaact tctcgaactg tccggtaagg aatccccgcc ccagcgggct gtaggcgacg
      781 aagccgatcc cgagctcgcg cacggtcggc aggatctcat cctccgggtc ccggctccac
      841 agcgagtact ctgtctggag cgccgtgacc gggtgtacag cgtgagcctt gcggatggta
      901 tcgggcgcgg cctcggagat gcccaggtaa cgtaccttgc ccgcctcgac cagttccttc
      961 atggcgcccc aggtctcctc gaccggcgta tccgggtcca cccggtgctg gtagtagagg
     1021 tctatgtaat cgataccgag gcgcttcagg gagtcgtcgc aggctcttct cacgtactcg
     1081 ggcctgccat tgatgcctag ccgggctccg tcttcgcccc gctcgttgcc gaacttggta
     1141 gcgagcacca cgtcgtcgcg ccggtccgct atcgccttgc cgacgagctt ctcgttggtg
     1201 aacgggccgt acatatccgc cgtgtccagg aagtttatgc cgagttccag agcgcggtgg
     1261 atggtggcta tcgactcgtc ctcgtcgggg gtgccgtaga actcgctcat ccccatgcac
     1321 ccgagtccca gctccgagac cacgagtccc tggcttccga gagtgcgctg tttcatacgt
     1381 gcctccttct caaatcgccg tgcccgcccc gtggaaataa gatcgcggcc agcgggctca
     1441 tgaagatcct tgcagtacct tgccgtgtcg ccgcggacca agttttacca tcacccgtac
     1501 ttcctaaaca ggctgcgccg actgtgccgg cagagtctac ccgccgtttt gcccgccgga
     1561 acccggcgac ccggcggagg gtgctacaga atactccgga aggcgaggat ttccggtctc
     1621 gcatcgaaac gtctaatcaa tttgggctag aatttaatcc ccctattggt ggtagcggag
     1681 ccaagtattt aggaatatct ttctggttga cgagtaccag ctaactcacg agaggcgttc
     1741 gtcgtgaaga actcgcgaca cagtaaaccc ggcgcgtaca acgtggcagg tctcgcgtgg
     1801 acgcgcctcc gtatcggacc catagacctg gccctcgtcg cgacgacggc ggtgatgctg
     1861 gcgacgatgg tgtccgccgc gaagttgacg ataggctgct tccacgtggt cttcgtattg
     1921 ctgaccttcg gggcgttctt ctggaagttc agggccttcg tgtggcgggc ggtcttctgg
     1981 gtcgctgtgg acacgggagt agtattgctg gcggccgtgt atggctcact cccttacgat
     2041 gagcttatcg agattccgct cctcagcatt atcctggtgc tggtgttcat gatcgcccgc
     2101 cgccgggccg aagcgcaggc gcggctgcag gagcaggacg aagccttcct cgaagcgatc
     2161 ctcggtaaca tggaggacgc ggtcgtggca tgcgacgccg ggggacggct ggcattctcg
     2221 aaccccgccg cccggacgat ccacgggctg cccgaggggc tacccgaggg gttgcccgag
     2281 gaactgccgc tggagcaatg gaccgatcac tacgagctac ggacggagac cggcgctacc
     2341 gtggacgaag cggatatccc gatactccgg gccctccggg gagagaggat aaaggacgag
     2401 gagttcgtga tctcgacgaa ggacaatccg gcgcgctatc tgctggtcag cggaaaccgc
     2461 atgcagggca gcctggaaca gacgctcggg gcggtagtag tgatgcgcga cattaccgag
     2521 cgcaagctgg ccgaacgcag gctgcgcgag gtgcaggaag acgagcgcga gcgcatagcc
     2581 cgcgacctgc acgacgcggt cttgcaggac ctctcgtggg ccatgcggaa gatcgagtac
     2641 gaccgcatga tggggctcgt ggacctgaaa aacgacctcg cctccgagca ggtggtgccc
     2701 gctctcagac gcgcggtcca gggggtacga aactcgatct acgacctatt ccccagggat
     2761 gcctcgaagc taccgttcgc ggagctgctg gagtccgcgc tggatctgaa ccgcgaaata
     2821 tcgccggaga tagatttcga ggccgtgatc ggcgagttcc cgcggaagct cccgcagccg
     2881 gcgggtgcgg aggtaatgaa gatcatccag gaggcgctgg tgaacgcccg gcgccactcc
     2941 tcagcctccc gcgtgcgggt aacgctcgac atcgaggggg accagctcgt cgtgtgcgtg
     3001 gaggacgatg gtagaggctt cgagccccac acggttcccg gactggggat ggcgggcatg
     3061 agagagcggg ccgagagact cggcggcagc ctggagatcc acagccgcga gctggagggt
     3121 acccgggtgt gcttcagcgc cgcgaaagag cgcctgacac acccccaaca gccgcaagag
     3181 aagctgtcct gggtctcggg aaagcagccg ggtagctcgt agaaggctga gaacaccgtg
     3241 atagaaaacg ccgtgataga agctcgccgc gaaagtggat gtaagccggg gtgtccggcc
     3301 ggggatagag aaggagccag atggccgaag agcccataga atcagtccgc atcatggtcg
     3361 tcgaggacca tagctcgttc cggcaggccc tggcgcacat gctggacctg gaaccagggt
     3421 tctccgtggt cgcggaggcc ggtacgctgg cagaggcgcg taagcgctcc aaagagaagc
     3481 ccgatgtcgc catcgtggat atggcgctcc ctgatggcga agggaccgag ctcatccgcg
     3541 agcttcacaa gtccggggac cgggtgacgg cgcttgtgct cagcgggagc ctcagccgcc
     3601 ggcagtacgc ccgggcggta gaggcgggcg cagccggggt gatgcacaag acgtcgagca
     3661 tcgaggagat cgtggacgcg gtccggcgcc tgaaggcggg cgagtctcta ctctcgctgg
     3721 acgaagtcgt cgcgctattt cgcgacgtgg gccaggagcg cgagcaaaac cgggacgcga
     3781 agcaggctgt ggagagcctc agccagcgcg agcgggaggt gctctcggcg ctcgcggagg
     3841 gactcgacag cagagagatc gccgagcggc tcaacatcac cgtggacacc gaacggtcgc
     3901 acttcatcag gatcttcgcc aagctcggcg tacactcgcg gttgcaggcc ctcgtcttcg
     3961 ccgtgcgcca cggcatcgtg gagatccact gataccggcc cgtccaggac tacagtctca
     4021 aactttagac catttggtgt aaagaaagtt aatgtgtatg gacgatgacg ggctctgcgg
     4081 gtctgtacac aataggtcca tgcgaaagac gcgcctcctg ctggtcaacg agcctctctc
     4141 ctaccgcgac gccattgctc acgtcttccg ggagcttcgc ccgaacctgg aggtgctggc
     4201 ctgcgatccg gatgatgtgg aatccggcct ggaggccctc gctcccgacg tcgtgatctg
     4261 cagcgcgctc acacccgcca ttcgagacgg ggtcagggtc tggatcgagc tttaccccgg
     4321 aggcgagccg ttggccgtgg tgagtatcgc gggagagcgc gccaccttct ccgacataga
     4381 actgccggac cttctctcgg tcgtggactc gacgaccgag atactccagc acaacctcta
     4441 actctctgca cgagcggctt ttcctgtctc tacgcctccg cgggtcggtg ccggccgcgc
     4501 tcacccgagc ctctgtcccg gcgagcgcgc gggagagcgt tcaatcgttc aatcaaatga
     4561 gttaatccag gcgcatcaag caaagttcat tgatctgcgc gatggcagac ccggcgctcc
     4621 attccattat tcagccacag gtcagccgag gaagggatac gaatgtcagc actacgggtt
     4681 tctggagaac gcgggagccc gcgctctcga agattcgacg cccggctcac agctgtggcg
     4741 gcgttgtcgc ttgttgcttc ccttctccac ctcctggtcg tcccggagca cttcgcggag
     4801 tggtggggct acggcgcctt ctttctgatc actgccctgg cccagggcat cggcggcgtg
     4861 gcgctactgc gctggcccat gcggcctctc ttcctcgcgg gcattgccgg caacggggcc
     4921 attgtggcga tgtggctcgc gagccggacg gtcggcatcc cgttcttcgg gccgcacgcc
     4981 tgggaggtag agggcgtcgg gaccatagac ctggcctgca cgatctccga ggtggccctg
     5041 atagccgcgc tcggcgcgct ggcgcgttcg aagaggcggc accagcgcga gacgagtgga
     5101 gcgtatgcga catgaccgga tttccgtgcc gcggggggat cccgcgcgga cctacgagta
     5161 gagccagaac agagatgagc aaggaggaag atcgatgaca ccggccaaga cgttgaaggt
     5221 tcggagatgg gacagggggg cgcggcttct cgcggccctt cttctgctgc tcgcggccgc
     5281 gctgctcgtc tcgcagtcgc ccgcgctggt acagcaggcg ggcgcgcaga cctccggccc
     5341 gtcttctctg gtcgtcaagg acctgaagac cggagacccc attgctaact tcaactacac
     5401 catcaacgcg gacaacgtcg gggacccggc ggacccggcg aacatgccgt ccgtcataaa
     5461 gggccagagc aacagccccg ttgcggctgt cggggatcag ggcagcgccg gaagcgtaga
     5521 cctggacccc ggcaagtacg tggcgagtgt ctcggccgac ggctacaagc tcggcggcga
     5581 gcacttcagc gccggggaca ccgtaacggt gttgctggag ccggagccga aggcgggcct
     5641 agatccctcg cggctcgcca agatccgggt ccacgccttc gaggacaacc ggatggtcaa
     5701 cggcgagaac gacgctcccc tggagtccgg tctcgaaggc ttcgacgtcg ttatcaagga
     5761 cgaggtcggc cacgttacgc aggactacta cggcaacgcc atcggcaccg agtacgagaa
     5821 ggacgccaac ggcgacctcg tgctcgaccc gaacggccag cccatcccgg tcccgaacac
     5881 gggcgggaac atactgacgg atgccaacgg cgacgcggtg gtcgagaacc tggcgccggg
     5941 caagtacggc gtccaggtca tcgcgcccga tgggtctggg tggatccaga cctccaccct
     6001 tgaaggcacg cacaccattg acgcctggat agacgaggga aacgacggca cgatcagcga
     6061 ggtccccggc gtctccccct cgccgatggc ggagatcggg ttcgtcaagg agcaatcctt
     6121 cgccaacccc ggaagcggca gcataaaggg ccgcgcccgc accgccctcg aatacatcac
     6181 cgaggcggcg aacccgccgc tcggagaccc ggtaaagagc ccctacatag cgctcacggc
     6241 gctcgacctg aacgacgagc tctcctacct tggacgcggc aacccggacg gcaccttcga
     6301 catccagaac gtcccggacg gcaagtatca gctcacgatc tgggacgagc cgatggacta
     6361 catcatctcc ttccgcaccg cgaccgtctc cggcggcaac gtcgttgaca tgggcgacat
     6421 cggtgtcccc cgctggttcg gcaagaccct cggcaccgtc ttcgaggaca acggcaagga
     6481 cggtaacggc aaccccataa cccgcgccga cggcacggac gcggccggga accagatcct
     6541 ggactgcgac cagaacgatc cgaatagctg cgagaagggc ctgccgggcg tggacgttgg
     6601 gacccgctac aaggacggct ccacccagta cggcaccctc accgacgcgc agggccacta
     6661 cgcgctgaac gagaccttcc ccctcggccg cttcaccgtc gccgaggtca gcaacgtccg
     6721 ctacgcgccg acaggagcct ccgtccgcag cgagtacaac ggcaccgacc tcaagagcct
     6781 ctcggtaccc ggcgccctgc tgatgagcac cttctcctgg accggcaaga actacataga
     6841 ctggggcaag aaacgctaca ccgggagcga gaacggcggc atctccggca tcgtctacta
     6901 cgcgacgatg cggaccgagg agaacgcccg ctaccagagc ggcgacccct atgagccggg
     6961 cgtccccggc gtgaaggtgg acctctacca ggccgacacc gacgccaacg gcgacccgat
     7021 caaggaggcc gatggctccg tcaagaccaa gaccgccgac ccgatagcca ccgtcaatac
     7081 cgactcctgg cagcagccca cgggttgcga cgtgctggac gagaacggca acacccccac
     7141 ggacccggcc tacgcgccga ccgcgcagca gatagcggac aactgcctcg aagtgcccgc
     7201 cctgggtaac gagacccgcg acggcgtctt cgacggcggc tacgccttca gcacctacac
     7261 ggacccttcc gacgggcaga ccaagccgct cccgccgggc gactacgtcg tcaagatgcg
     7321 gatacccaag gacaagaacg gcaacgacct ctacaagatc gtcaaggaag aagacgtaaa
     7381 catcgagaag ggcgagaact tcgtcccgca ggtgccgccc ccgccgtgcg ccggcgcgct
     7441 gcacaccgtc aacgacccgc gccagggtcc ctacgacggc cagcagatgc cgctgtgcga
     7501 cagcaagctc gttcacctga acgcgcaaca gaacgcgggc gccgacttct tcatgttcac
     7561 cgacacgccc ataccgggcc gcgtctgggg tctcgtcacc gacgacctga acatcgagac
     7621 cgacccgagc ctcctctact acggcgacaa gaagggcatc gcccacatcc cggtcggcgt
     7681 ctacgaccac accggcaggc tcatacagac cgtctacacc gacgataacg ggatcttcga
     7741 gacgatcctg ccctccacgg ggaccttcaa cgtccccacc ccctccggcg tctctcccaa
     7801 catgtacacc ttcctcgcca acgaccccgg cccggacccg aacaacccta accccgtcca
     7861 gggacccgga accgtcatgg gctacgaccc aaactacagc acgctgcccc tgatctacga
     7921 cgtctggccc ggcaagacga cggccgccga catggcgatc ctgccgaaca cccctggtgg
     7981 ggatcccggc ggcgcgcagg cgggagagcc ggaggacggc gcgcccgacg tgcaaaacgt
     8041 ccagaacccg gtcgtccagg ccgcgggcgc caggaacctc gtcatcaagg gcaaggactt
     8101 cggcgctgct cctcaagtca ccctcgacgg caagcagata acggtcaact ccatgagcgg
     8161 cgacacgcag ataaacgcgg ccgttccggc gggcttcccg gtcggaccgc acgagctgct
     8221 cgtcaagtcg ggcggcagga ccagcgcgac gggcctcaag gttcatgtgc tgggcacgtc
     8281 cggcaaaact acctacaggc cgaccgtgat ccgagtcaat caggggcaaa agatccagga
     8341 cgccataaac cgggcgagcg gcaacaccct gatcgtggtc ggacccggaa cgtataacga
     8401 gaacctgatc gtgcacaaag gcgtgaagct acagggcgtc ggccccggca gcgtcaccat
     8461 taacggcagc tacgtggacg cccactcggc ggcctggcac agcctcctcg gcagcgtcaa
     8521 cttcgacggc gacaggaacg tcaacgaagg atcggcgatc accgtcctcg ccaaagcggg
     8581 ccagttcacg ccgaccttcc gtccctccat cgacggcttc accatcaagg gcgggcgcgg
     8641 cgactccggc ggcggcatct tcgtcaacgg gtacggccgc tacctcgaca tatccaacaa
     8701 cgtcatccag ggcaaccgcg gctactacgg aggcgcggtc acgctgggac ggccctactc
     8761 cggcgacgct cacaacacga acgtccgcat ccgctacaac cgcgtgct
//
LOCUS       DASSMB010000259         5311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_210427, whole genome shotgun sequence.
ACCESSION   DASSMB010000259 DASSMB010000000
VERSION     DASSMB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210427"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VFJ72_14760"
     CDS             complement(<1..1074)
                     /locus_tag="VFJ72_14760"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7480764.1"
                     /translation="MSWTVALLSLLAGFVAAGRLVPAVRWFLRQQSERVVEEAGRRGI
                     KFPTFKLTRRRALIRRLTHDQEILRAAQTYADANGVSMASAMARVERYAREIVPSFNA
                     FLYFKVGIPLADRVSRSLYDVRVVSDEGLAAVDEGANEGASVVFVMNHRSNMDYVLLA
                     HLTAHRTALSYAAGEWACVWPLGDLIGGMGAFFVRRGSGDTLYRRVLERFVQMAVEGG
                     LVQVFFPEGGLSRDGKPREPKIGLLDYMLRRFDPQAGDILFVPVGVNYHWVLEDHSLL
                     QPGGPEAGVRGRGGLFASTGVSVARNLLRARRAGRFRLGTAAVGFGAPVSAREYAAAR
                     GVEFREMEREERIEEVKRLARRLM"
     gene            complement(1294..2046)
                     /locus_tag="VFJ72_14765"
     CDS             complement(1294..2046)
                     /locus_tag="VFJ72_14765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7480765.1"
                     /translation="MTTLNGKIALVTGGAGGIGVGICEVLAEHGATVIVGYNSSRDEA
                     VTLVERLPNPGAGHAAYAAPVTDSAALSELARKLEQHHGRLDILVNCHGTTRFVPAED
                     LDDLDDELIDRVLATNVRGTFATVRALKGLLARDGDGLVVNISSIAAKLAIGSNIMYC
                     ASKAAVDTLTKSLALALAPRVRVVSVSPGLVLTEAAKKFDPDFLESHISRTPLGRLTT
                     PRDIGATIVAVATLMTAVTGVVIPVDTGRSLS"
     gene            complement(2393..3505)
                     /locus_tag="VFJ72_14770"
     CDS             complement(2393..3505)
                     /locus_tag="VFJ72_14770"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7480766.1"
                     /translation="MAFEPSLSTPLCELLGIRYPLIQAPMAGGWTTPELVAEVCNAGG
                     FGMLAGARVSPEKLREDIRTVKELTDRPFGVNFLIAPPGEGNPDVGSVQRFLDRFRER
                     LDLPAGETDIALPPSPLREQLEVVFEERAAVLSVALGEPGDLVERAHDQGMLFSAMVT
                     TVEEAVLVAERGADVIVAQGAEAGGHRSTFELGPNREAPLVGTMALVPQVVDAVDVPV
                     VAAGGIADGRGLLAALALGADGVQVGTRFLSARESGVHPAYRERLLSSTETDTVVTHV
                     FTGRPARGFRNRLADEYLREGPEPLGWILHGMAAGDIYAAAQAADNGDYSPLLAGQGL
                     RLLKDGQGAAEIVAELMEEAGNALSRLSGGAHSETR"
     gene            complement(3599..4819)
                     /locus_tag="VFJ72_14775"
     CDS             complement(3599..4819)
                     /locus_tag="VFJ72_14775"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET7480767.1"
                     /translation="MLTEFAGTPGKEAYTRLERETLEAVVTNRGAARARSGHPWIYRS
                     DVADASGEPGDVVRVVDRRGSFLGRAFYNPRSQISLRVATREDEPLDEAWFRNRLRRA
                     IAYRDSLHIDADVYRLLHSEADRLPGLVVDRYGNHLVLQIGSAGVERRLEWVVAALQD
                     LQASDGILLRGDTTARKREGLDVDVRVLAGDVPESVVVREGPVRYRADLWGGQKTGGF
                     LDQRENHLAAGRYSRGRVLDVFSYAGGFALHAARNADTVECVDASGPALEAARANAAL
                     NGYENLTFIRANAFDLLREISDAGERYDTVILDPPAFAKTKRELPKARRAYKEINLRA
                     MKLLAPGGVLVTCSCSYHFTRDLMEETLRGAAADAGATMRLREWRGQALDHPEILTIP
                     ETHYLKCAILERMA"
     gene            4874..>5311
                     /locus_tag="VFJ72_14780"
     CDS             4874..>5311
                     /locus_tag="VFJ72_14780"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7480768.1"
                     /translation="MTAARKRVVIVGAGMGGLAAAIRLSALGFEVEVFEKNPTVGGRM
                     NRLEAEGFTFDTGPSLLLMTDTYRELFRFAGRDLDDYVNLIPLDGQYRVTFGDGDTLT
                     IRRALPELIKEIERIEPGVTPNFYRFLEDACYKYRLGRSEFVER"
BASE COUNT          888 a         1884 c         1692 g          847 t
ORIGIN      
        1 catcagacgc cgggcgagtc tctttacctc ctcgatccgc tcctcgcgtt ccatctcccg
       61 gaactccacc ccgcgcgccg ccgcgtactc gcgggccgag accggcgccc cgaagccgac
      121 ggcggctgtc ccgaggcgga atctaccggc gcggcgcgcc cgaaggaggt tgcgtgctac
      181 cgagacgccc gtggaggcga aaagtccgcc ccggcctctt actccggcct ccgggccgcc
      241 gggctgcagg aggctgtgat cctccagcac ccagtggtag ttgacgccga cgggcacgaa
      301 gaggatgtcg ccggcctgcg gatcgaagcg gcgcagcatg tagtccagga gccctatctt
      361 cggttctcgg ggctttccgt cccggctcag tccgccctcg gggaagaaga cctgtacgag
      421 accgccttcg acggccatct gcacgaagcg ctccaggacg cgccggtaca gcgtgtctcc
      481 cgacccgcgc cgcacgaaga aagcgcccat acccccgatc aggtccccca gcggccacac
      541 gcaggcccac tcccccgccg cgtagctgag agcggtgcgg tgggccgtga ggtgggccag
      601 caatacgtag tccatgttgc tccggtggtt catgacgaag accacgctcg cgccctcatt
      661 cgcgccttcg tccaccgccg cgagtccctc gtcggagacg acgcggacgt cgtagaggga
      721 gcgggagacc cggtcagcca gcggtatccc gaccttaaag tagaggaagg cgttgaagga
      781 cggcacgatc tccctggcgt agcgctccac gcgggccatc gcggaggcca tggagacgcc
      841 gttcgcgtcg gcgtatgtct gggcggcccg cagtatctcc tggtcgtggg tgagtcggcg
      901 gatgagagcc cgccgccggg tcagcttgaa ggttgggaac ttgatacccc gccggccggc
      961 ctcctcgacc accctctcgc tctgctgccg caggaaccag cgcaccgcgg ggaccagccg
     1021 tccggccgca acgaaaccgg cgagcaggga cagcagagcc accgtccagc tcaaccggct
     1081 tctcctatcg ctcacggctc tccccgcatc cgatcccctg acacgaaccg cagtatagcc
     1141 ggatacggga agccggcgtc cacatcggcg cgccacgagc tcacatcatc cgtagcccgg
     1201 cacgataggc gcgggggaac cgtccacacg gcctcagcta ttgccccagt cgggaaacac
     1261 gaccggccga gccgcgccga accgcaaagg tctctagctc aggctgcgcc ccgtatcgac
     1321 cgggatgacg acgcccgtca ccgccgtcat caacgtcgcc acagccacga tcgtggcccc
     1381 gatgtcgcgc ggcgtggtca ggcgcccgag cggcgtgcgg gagatgtggc tctccaggaa
     1441 atcggggtcg aacttcttcg ccgcctccgt caacacgagg ccgggagaga ccgagaccac
     1501 acgaacgcgc ggcgcgagag ccagcgcgag cgatttcgtg agcgtgtcca cggcggcctt
     1561 cgaagcgcag tacatgatgt tggagccgat ggcgagtttc gcggcgatgg acgagatatt
     1621 caccaccagg ccgtcgccat cgcgcgcgag caagcccttg agcgcgcgca cggtggcgaa
     1681 agtgccgcgc acattcgtcg cgagcacgcg gtcgatcaac tcgtcgtcga gatcgtcgag
     1741 gtcctcggcg gggacgaagc gcgtcgtgcc gtggcagttc accaggatgt ccaggcggcc
     1801 atgatgctgc tccaacttcc gcgccagctc cgacagcgct gcgctatcgg tgaccggcgc
     1861 ggcgtacgcc gcgtgtcccg cgcccggatt cggcaggcgc tcgacaagcg ttaccgcttc
     1921 gtcgcgcgag ctgttgtagc cgacgatcac ggtcgcgccg tgctcggcga gcacctcgca
     1981 gatgcccacg ccgatgccgc cggcgccgcc ggtgaccaga gcgatcttcc cattcagggt
     2041 cgtcatccat tctcctccaa acttcagcgt cagttgcaat aaccagctct cttatgagcg
     2101 cgtatcgcga atatgtcagg ggcgcggacg ccttcgcgaa cacctgctct cccggcaccc
     2161 gggcgacggc cttgaggtac tctcgcggtt cctccagact cggtggcaca acggcaggct
     2221 tcggctactt gcccccgtac tcgactcccg cttcgattgt cctcgatggg gcggcggcca
     2281 ttcaactccc gctggatagc tcgccgcacc tgtagtaccg ggatctcgcc accggacgtc
     2341 cgacaacagg aaccgcccga gcggcgtgag atcttcaccc gagagatacg ccctagcggg
     2401 tctccgagtg cgcgccgcca ctcaggcgag acaacgcatt cccggcctcc tccatgagtt
     2461 ccgccactat ctccgccgct ccctgcccgt ccttgagcag ccggagcccc tgcccggcga
     2521 gcagcggcga gtagtcgccg ttgtccgccg cctgcgccgc ggcgtagata tctccggcgg
     2581 ccatgccgtg aaggatccag cccagcggct cggggccttc tctcaggtac tcatcagcca
     2641 gccggttgcg gaaccctcgc gccggacgcc ccgtaaacac gtgggtaacg accgtgtccg
     2701 tctcggtgga gctcaaaagt cgctcgcggt aggcgggatg cacgccgctc tcccgcgccg
     2761 acaggaaccg ggtccccacc tggaccccgt cggccccgag cgccagagcg gcgagcaggc
     2821 cccggccgtc cgcgatgcct ccggcggcga ccaccggcac gtccaccgcg tccacgacct
     2881 gcggtaccag cgccatcgtc ccgaccagcg gcgcctcgcg gttcgggccc agctcgaacg
     2941 tggagcggtg ccccccggcc tcggcgccct gggcgacgat cacgtccgcg ccgcgctccg
     3001 ccacgaggac cgcctcctcg accgtcgtga ccatcgccga gaagagcatc ccctgatcgt
     3061 gcgcccgctc taccaggtct ccgggctctc ccagcgccac gctcaacacg gccgcccgct
     3121 cctcgaacac cacctctagc tgctcccgaa gcggcgaggg cggcaacgcg atatcagtct
     3181 ctcccgccgg caggtccagc ctctcccgga accggtccaa aaaacgctgc acggacccta
     3241 cgtcggggtt cccttcgcct ggaggtgcga tcaggaagtt gaccccgaac ggcctgtcgg
     3301 tcaactcctt gaccgtccgg atgtcctccc gcaacttctc cggcgacacc cgcgcgcccg
     3361 ccagcatccc gaaccctccg gcattgcaga cctcggcgac aagctccggc gtcgtccacc
     3421 ctccggccat cggagcctgg atgaggggat agcgtatgcc gagaagctcg cacaacgggg
     3481 tcgagagaga aggttcaaac gccacaacag acctccttgt catagagttc cggcaaggtc
     3541 cccaccgtac ctgagaacta cttacgccat cacggtcacg atactctaca cgcggctctc
     3601 atgccatccg ctctaagatg gcgcatttga gatagtgggt ctcggggatg gtcaggatct
     3661 ccgggtggtc cagggcctgt ccccgccact cccggagccg catcgttgcc cccgcgtccg
     3721 ccgccgcgcc gcgcagggtc tcctccatga gatcgcgcgt gaagtggtag gagcaggaac
     3781 aggtgacgag cacgccgccg ggggcgagaa gcttcatggc gcggaggttt atctccttgt
     3841 acgcgcggcg cgccttcggc agctcgcgtt tggtcttggc gaaagcgggc gggtcgagga
     3901 tgacagtgtc gtagcgctcc ccggcgtcgg agatctcgcg cagcaggtcg aaggcgttgg
     3961 cgcggatgaa ggtgaggttc tcgtagccgt tgagcgcggc gttggcgcgg gcggcctcca
     4021 gcgcgggccc gctcgcgtcc acgcactcca ccgtatcggc gttgcgggcg gcgtgcaggg
     4081 cgaaaccgcc agcgtaggag aatacgtcga ggacgcggcc gcgcgagtaa cgcccggcgg
     4141 cgaggtggtt ctcgcgctgg tccaggaacc cgccggtttt ctggccgccc cagagatctg
     4201 cgcggtagcg tacggggcct tcgcgcacaa cgacactttc gggtacgtcg cctgccagga
     4261 cgcgcacatc cacgtctaaa ccctcgcgtt tgcgggcggt ggtgtcccca cggagcagga
     4321 tgccgtccga cgcctggagg tcctggagcg cggccacgac ccactccagg cgccgctcta
     4381 cgcccgcgga cccgatctgt agcacgagat gattcccgta gcggtccacc acgagccccg
     4441 gcagcctgtc agcctcggag tggaggagcc ggtagacgtc ggcgtctata tgtaaagagt
     4501 cgcggtaggc gatggctcgt cgcagtcgat tacggaacca ggcttcgtct aatggttcgt
     4561 cctcgcgggt ggcgacgcgg agtgagatct ggcttcgggg gttgtagaag gcccgtccga
     4621 ggaagctgcc cctgcggtcc acgacgcgga cgacgtcacc aggctccccc gaggcatcgg
     4681 ccacgtcgga gcggtagatc caggggtgtc cagaccgggc gcgggcagcg ccccggttcg
     4741 tcacaacggc ttccagcgtc tctcgctcca acctcgtgta agcctcctta cccggcgtac
     4801 cggcgaattc tgttagcatc ttcagcgtat acttgaatat acctttatct cgcggaggaa
     4861 agacggtgga tcggtgacgg cggccaggaa gcgtgtggtt atcgtggggg ccgggatggg
     4921 cggactggcc gcggcgatcc ggctgtcggc actgggcttc gaggtcgagg tcttcgagaa
     4981 gaacccgaca gtcggcggac gcatgaaccg cctggaagcc gagggcttta ccttcgacac
     5041 cggtccctcg ctcctcctga tgaccgacac ctaccgcgag ctgttccgct tcgccgggcg
     5101 ggatctcgac gactacgtga acctcatccc gctggacggg cagtaccgcg taaccttcgg
     5161 ggatggcgat acgctcacga tccggcgcgc cctcccggag cttataaaag agatcgaacg
     5221 catagaaccc ggcgtcaccc cgaacttcta ccgcttcctc gaagacgcct gctacaagta
     5281 ccgtctcggc cgctccgagt tcgtcgaacg c
//
LOCUS       DASSMB010000260        14661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_350764, whole genome shotgun sequence.
ACCESSION   DASSMB010000260 DASSMB010000000
VERSION     DASSMB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 14661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14661
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350764"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VFJ72_14785"
     CDS             <1..363
                     /locus_tag="VFJ72_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480769.1"
                     /translation="AMPTSTQITLGNDLDGNRKIACPNTQSKCEYITYRLSGATLLRN
                     NTANASSGGDAVAENVKDVDGDGKALTFSYMKSNGTTATSESDIRIVRVKLEVSKSGG
                     SFDDGTQTVTTDIALRNR"
     gene            370..2001
                     /locus_tag="VFJ72_14790"
     CDS             370..2001
                     /locus_tag="VFJ72_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480770.1"
                     /translation="MVRRLLKDESGMTMALAVMMVVIIGVMGAGLLTFTMRDLDTVVQ
                     TNQGQTAQAMADAGMQAAKLQLHTMDSNPLHYDGVSNATNTESSWSRTASDPTTWKHM
                     TFGGNDMWVDIKYLPPSSNATQASDKNFAPVILPSGATDSNRDPDTDPDYPSDRHYFR
                     VTVEANADGAVRWYQAIYQTENYNFPVSYFATRDINVTGASATVNGVSLFAQRYITNL
                     TPDSKTGSKVKCGTICGVDQAYSNWATNPDGTANGYNKTARPTTSAGAAALGGTPPTA
                     ACSSAAISRDSGIGYASASDNTNQKKMSADPQDYGQRDYDRDSDFKCPSSTAVSSGRP
                     EFTLNTWTASGATQPSTKITFPFAVNDSSTDAAILSSLKDKAQAQNHYVQVDNGSSSK
                     EIDIADSTGFNTPSGAVYPPNSTLDTVMYIEFTGSVKGSVLYKTGGNGDQWKGTIVVI
                     NGDLDTSASSASYQGVMIVRDGVSNGTATTESQITTFNNSGSLNIDGYVNVEGDLFLQ
                     GSVGGVFPGELLNGIPGVVTVNRWSWRECYAANCP"
     gene            2269..2601
                     /locus_tag="VFJ72_14795"
     CDS             2269..2601
                     /locus_tag="VFJ72_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480771.1"
                     /translation="MTRVRLSVDVDPELRRRVKVVAAARDQSVKEWVEGVVREALIRE
                     YREATEYAPGPPDSDEDRAWLDNDLSGLGEFESYEWEEGELEEGRPVSYEPGVGAVVE
                     GGKQRGGR"
     gene            2588..2986
                     /locus_tag="VFJ72_14800"
     CDS             2588..2986
                     /locus_tag="VFJ72_14800"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET7480772.1"
                     /translation="MAEGNGESGERLEIGDVVSALFPLHRPGGREQEGYRPAVVVGLP
                     EFAGTPRFGALIFTPMTTDRGQAWARRSPELYPRYPEGTANLKSPSVCLLDQVRALDV
                     GRVRDYRGTLSTEEYRPIGKGLAKMIRDPR"
     gene            3301..3612
                     /locus_tag="VFJ72_14805"
     CDS             3301..3612
                     /locus_tag="VFJ72_14805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480773.1"
                     /translation="MISTERRVVTFVWRCTDCASVYDGESLLFLHGDGFNRYCSCGGR
                     LVEDVESRLERHCRLVLPEHLGGTRLHRQLERRLSGEDSVEVSTGRGDDGRIHLFNCA
                     N"
     gene            3623..4000
                     /locus_tag="VFJ72_14810"
     CDS             3623..4000
                     /locus_tag="VFJ72_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480774.1"
                     /translation="MSGRAPAGSRALLLSHLLRAVLFGGLAGWISSLLGGVVDGWVLY
                     LGCAALAGAAGWVLARPMERNSLLAVLVLWLVAGLLYYSLGTPVEAGLLGVSVGLGEY
                     VELGGVVIGLGIGALLGRAVARP"
     gene            4050..4517
                     /locus_tag="VFJ72_14815"
     CDS             4050..4517
                     /locus_tag="VFJ72_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480775.1"
                     /translation="MEERETRGGALRVGMRWGGIGGIVGFAASLLGSLVGILVAGFVG
                     VACGRRAAEADGGDSAGAGALAGLVGGAVAAPVYVVGASAGALVAARTVEMGMVASML
                     SEMLGTSITSEEAWRIFLIAVVFAAAVQAAVFILTSVAAGAWVKRNSGQAPGL"
     gene            complement(4562..5038)
                     /locus_tag="VFJ72_14820"
     CDS             complement(4562..5038)
                     /locus_tag="VFJ72_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET7480776.1"
                     /translation="MDRQLQIQILLDHYEKPRHRGPLEDADVAMPGGNPGCGDVVTVY
                     LKGAGDQEHIEDVSYEGEGCTISMAASSMILEQVHDGDLTMDEVLQMDYNEMIEQLGR
                     QIVASRPKCATLGLGTLKAAIRKYQRDQRLDAAGVTRPEDEGLVFGEGAAEAAKQQ"
     gene            5190..6125
                     /locus_tag="VFJ72_14825"
     CDS             5190..6125
                     /locus_tag="VFJ72_14825"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7480777.1"
                     /translation="MRGRDIGLLLALGAMWGSSFLFIKVALGEVAPAFIVACRVFFGA
                     LTLLAAVPFLDRIFGKTPDGLLRSVARLWRPVLVLGVFNAALPFLAISWGTQFLPSGT
                     AAILNSTVPLFTALLAGLLPWITDERLGVLGVAGILLGVAGVAVLAGGFGEGFSASPS
                     EALLGAGAIMTGSTSYAVGGLYARRAMKGVPVLVSAVGQSVAALVVILPFAALSLPDT
                     VPSLPVLGSLVGLGAIGTGFSLLLYFKLIANVGATRTSTVTYLVPMWALVYGALLLGE
                     DITVRAVLGLVLILLGVAGVTGFARLPRRRPPAAP"
     gene            6153..7235
                     /gene="ychF"
                     /locus_tag="VFJ72_14830"
     CDS             6153..7235
                     /gene="ychF"
                     /locus_tag="VFJ72_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET7480778.1"
                     /translation="MRVGIVGLPNVGKSTVFNSLTRAGAEAQNYPFTTIDPNLGVAVV
                     PDVRLDALAEVMKSRSVVPATVDFVDIAGLVRGASAGEGLGNQFLGHIRECEAIAHVV
                     RCFEDENVVHVHGGVDPVGDIETIQTELLLADLATVERRLERTSKAAKSGDAKLRAEA
                     AELEKLREHLSDGSPARTYPESEAIAEAMGTLITAKPTLYVANVDEESVAEGNRYSAE
                     VEKLAAREGAEVVRLSARLEAEVLDLPEDEAREYLEMLGVEKTGFEEFVRAAYSLLGL
                     ITFFTAGEKESRAWTVKKGATVRRAAGGIHSDMERGFIAAEVADWRDVVSAGSWSKTK
                     EEANMRLEGRDYLVRDGDTLLVRFNV"
     gene            7250..7645
                     /locus_tag="VFJ72_14835"
     CDS             7250..7645
                     /locus_tag="VFJ72_14835"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HET7480779.1"
                     /translation="MPPFVHPTKPLAESRVALVTTGGVHLPEQPRFDIDDPLGDCSYR
                     EIPVGAEELTWTHAYYRPDEGADLDAVFPLWTLRRLAAEGVVGRPNHRHFSFMGAIHD
                     AGPLIEETAPEVARKLVEDQVDAALLTPS"
     gene            7721..7915
                     /locus_tag="VFJ72_14840"
     CDS             7721..7915
                     /locus_tag="VFJ72_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480780.1"
                     /translation="MERGVEAPRAAFVPFPYNCPMGAPGDDERHREVAMAALSLLHEM
                     DAPGEVPLPFKWRKGTGAGR"
     gene            7997..8686
                     /locus_tag="VFJ72_14845"
     CDS             7997..8686
                     /locus_tag="VFJ72_14845"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HET7480781.1"
                     /translation="MALLFVLFVAFFVVLFLFLLPILFSLQAVGSLFVYPRQLKAMFG
                     NRILRRNHALEHATIVVMMEREPGRKLNGFSTDDGFFVQGVRSVEEVESAAREALRRL
                     RAGESNLAIHRNCGTTIVAANLLTAILFLGALGLGLYMSWPLYLLILGSIAVALALRV
                     PLSLLLQRYVTTDADLTNAEVGWVEPAKPADLRSGILGVLLAASTARVRVFHTDPDAV
                     EIIRDDGAVVR"
     gene            complement(8683..9672)
                     /locus_tag="VFJ72_14850"
     CDS             complement(8683..9672)
                     /locus_tag="VFJ72_14850"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7480782.1"
                     /translation="MNHPPLRLGILGAARIALNAIIPAANRSETVEVSAVATRGGGKS
                     AGVRDLAPRAGIFEDYEALLASPDVDAVYIPLPNSMHVEWTLRAIDAGKHVLCEKPFS
                     QDAAAAEKAARAARRSGLTLMEGFMFRFHPQTLRLKELLAEGAVGEVRQVIAEFGHRL
                     DDPEDVRGVGSLGGGSLGDVGCYCVSGVRLAFGAEPRRAAAFGRFAEDGADREVAGVL
                     EFDGGVGTVSCSISSARRERMEIVGTEGRISLDAPFRADKSGSSLNITRGGETATESF
                     PEADPYLAELEEFAAAIREGREPAIGPDEILGNARAIEALLKSARSGGAARDL"
     gene            10066..10518
                     /locus_tag="VFJ72_14855"
     CDS             10066..10518
                     /locus_tag="VFJ72_14855"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7480783.1"
                     /translation="MEREHTGRHFTVAVFVVWEGKVLLHHHRKLGMWLPPGGHIEKDE
                     LPDDAALREVWEETGVRVALVGERREDVEDPVQLHRPAGVQLEDIGPGHQHIDLIYFA
                     RPTGSTGIHEDFSVDRVGWYAPADWDEMQVNAEVRGWCERALLAFEGA"
     gene            complement(10495..11133)
                     /gene="pcp"
                     /locus_tag="VFJ72_14860"
     CDS             complement(10495..11133)
                     /gene="pcp"
                     /locus_tag="VFJ72_14860"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET7480784.1"
                     /translation="MRATVLVTGFDPFGGEEINASGEVVKRLEDSTFDNAEVSTRILP
                     TDFSRAPRALEDAIRDSAPEVVICLGQARGRSQVTPERVAINAADARRIPDNADRRPL
                     DEPVAADGPAAYWSTLPVRELEATLLACGIPARISNTAGTFVCNQVFYSLMHLLATRR
                     SPTRGGFIHVPVLPEQALEEAAPSMTLALSVRAVELAIEVAVRAALRALKSQ"
     gene            complement(11138..11293)
                     /locus_tag="VFJ72_14865"
     CDS             complement(11138..11293)
                     /locus_tag="VFJ72_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480785.1"
                     /translation="MGDERTGEQTNAPEALAPSGEECEICGAEMYGLHCKLICPNCGY
                     RRDCSDP"
     gene            complement(11298..11693)
                     /locus_tag="VFJ72_14870"
     CDS             complement(11298..11693)
                     /locus_tag="VFJ72_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480786.1"
                     /translation="MAAILLILISLGAVLALISAVRALRAWLGYRRARAAFQTDVADE
                     VARLAKRAGELERGVASLDARASQLPIRISELQQSLAMLKTLTGVLGTSLRQAQRLLS
                     LTSVKTSGTAHLADILGKRWQSWKTRRAV"
     gene            11766..11839
                     /locus_tag="VFJ72_14875"
     tRNA            11766..11839
                     /locus_tag="VFJ72_14875"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11800..11802,aa:Met,seq:cat)
     gene            12025..12507
                     /locus_tag="VFJ72_14880"
     CDS             12025..12507
                     /locus_tag="VFJ72_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7480787.1"
                     /translation="MTEKSGTQTQRSPLHSDKGRTTISDAVVQQVAGIAAQEVEKVQM
                     GGGTSAAVGGFLQSVTGGITGGSTSGGGNYSKGVSVEVGEEETAIDLTMAIEYGKSIP
                     QVTEAVRRNVINRVESMVGLRVTEVNVTVSDVQTPEERPMLEQQEQTEQMARREEQRA
                     "
     gene            complement(12604..13806)
                     /locus_tag="VFJ72_14885"
     CDS             complement(12604..13806)
                     /locus_tag="VFJ72_14885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HET7480788.1"
                     /translation="MVLIASALAALMLWPASGASADTGRVVDETGDGAAYVAGELIVT
                     YKEDASSSVMEKLPEQVRARIEDDLPIVDAQLVEFPGLKDVSSKSEREDTLARKKREL
                     EKDPAVEAVDYNYIRKFSLTPDDPRFGEQYGLRQIKAPSAWDRTQGNSTVRIGLVDSG
                     IKASHPDLNGKVAAQHDFGNGDGVAEDTVGHGTHVAGVAAAETGNGTGVAGTCPNCKL
                     LMAKVDRRGEIDSAAVIKGIDWAADRGAKVINLSLGAPGFVEAEARAVQYAQDHGAVV
                     VAAAGNENSTVRSYPAAYRGVLAVAATNRSGTRASFSNKGDWIDLAAPGVNILSTAPG
                     GYSALSGTSFSSPYVSGVAGLLASQGRGPRAISRRLTSTATDLGPDGRDPYYGAGRLD
                     AAAAVGAR"
     gene            13981..>14661
                     /gene="tilS"
                     /locus_tag="VFJ72_14890"
     CDS             13981..>14661
                     /gene="tilS"
                     /locus_tag="VFJ72_14890"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7480789.1"
                     /translation="MKASEFPQRVARTAERYGMDLSRPLAMVSGGPDSVALLRALVEL
                     GGEPVVLHVDHGLRGDESREDARFVRDLCKRLGLVCEMREADLPARNLQEEARRERYR
                     MAEDVAAARGLSVIVTGHTSDDVAETVLLNLSRGAGLRGLAGIPPIRGRLVRPLIERR
                     RAEVLAYLKYLDQPYRTDPTNLTGKYARNRVRLEVLPVLEELYPGAGANIARAADLVR
                     QDLDALESM"
BASE COUNT         2472 a         4590 c         5031 g         2568 t
ORIGIN      
        1 gccatgccga cctccacaca gatcactctc gggaacgatc tggacggcaa caggaagatc
       61 gcgtgcccca acacccagag caagtgtgag tacatcacct acaggttgag cggcgccacg
      121 cttcttagga acaatactgc caacgccagc agcggtggag acgcggtggc ggagaacgtg
      181 aaagacgtgg acggcgatgg aaaagcgctc actttcagtt atatgaaaag taacggtacg
      241 accgcaacca gcgagagcga cataaggata gtgcgggtca agctggaggt ctcgaagagc
      301 ggcgggtcat ttgacgacgg cacccagacg gtcacgacgg atatagctct aaggaacagg
      361 taggctcgaa tggtacgccg gctcttgaaa gacgaatcgg ggatgacgat ggctctcgcg
      421 gtcatgatgg tcgtcatcat cggggtcatg ggggccgggc tcctgacctt caccatgcgc
      481 gacctggata ctgtcgtcca gaccaaccag ggacagaccg cccaggcgat ggccgatgcc
      541 gggatgcagg ccgccaagtt gcagctacac acgatggact cgaaccctct gcactacgat
      601 ggtgttagca acgccaccaa cactgagtcg tcctggtcca ggaccgccag cgacccgacg
      661 acctggaaac atatgacctt cggcggcaac gacatgtggg tggacatcaa gtacctgccg
      721 ccgtcgagca acgctacgca ggcgtcggat aagaacttcg cgcccgtgat cctccctagc
      781 ggcgcgacgg acagtaacag ggaccccgac acggaccctg actacccgag cgaccgccac
      841 tacttccggg taacggtgga ggcaaacgcc gacggcgcgg tgcgctggta tcaggccatc
      901 taccagacgg agaactacaa cttccccgtc tcctacttcg cgaccaggga cataaacgtt
      961 accggagctt ccgccaccgt aaacggggtc agcctgttcg ctcaacgata cataacgaac
     1021 ctgaccccgg acagcaagac cggcagcaag gtcaaatgcg gaaccatatg tggagtggat
     1081 caagcctata gtaactgggc cactaacccc gacggcacag ccaacggcta caacaagaca
     1141 gcacgtccga ccacgtccgc aggcgcggcg gctctgggag gcactccccc cacagcagct
     1201 tgctcgtcag ccgctatatc gagggatagc ggtatcggat acgcctcggc cagcgacaac
     1261 acgaaccaga agaagatgag tgcggatccc caggactacg gtcagcgcga ctatgatcgc
     1321 gacagcgact ttaaatgtcc cagcagtacc gccgtatcgt ctggcagacc ggagttcacc
     1381 ctcaatacct ggaccgcgtc tggcgcgaca caaccctcga caaagataac gttccctttc
     1441 gcggtcaacg actcctcgac cgacgccgcg atcctgagta gtctcaagga taaggcacag
     1501 gctcagaatc actatgtgca ggtcgataac ggcagcagct cgaaagagat agacattgct
     1561 gattcgacgg gcttcaacac gccgtcagga gccgtttatc ctccaaactc gacccttgac
     1621 accgtgatgt acatagagtt caccggaagt gtcaagggaa gcgttctgta caagacgggt
     1681 gggaacggcg accagtggaa gggcaccata gtggtcatca atggagacct cgacaccagc
     1741 gcttcgtccg ccagctatca gggcgtcatg atcgtccggg acggtgtcag taatggcact
     1801 gccactaccg agagccagat caccaccttc aacaacagcg gaagtctgaa catcgacggt
     1861 tacgtcaacg tcgagggaga cctgttcctt caggggagcg tgggcggagt cttccctggc
     1921 gagctgttga acggcattcc cggcgtcgtt accgtgaacc gctggagttg gcgggagtgc
     1981 tacgcggcaa actgcccctg aagccgacgc gatgtaggag ttgaagggag ccgcgcgcga
     2041 cgcggtccgg tatcacttcg agcctgcagg cggagccgcg gttggatcac agggcttggt
     2101 cctccttctg gtccatcgtg ttcaactcta tctagtgctc ggaggacatc ccgacgtgca
     2161 atcggcatag catcctgctc tccgtgagat cggaccactc tcgaagatgt aaaatggaca
     2221 tgacacagtg acacagaaca ttctgatttt gacggaaggc atttgaggat gacgagggtg
     2281 aggttgtcgg ttgatgtaga tccggagttg aggcgccggg tgaaggtcgt ggccgcggcc
     2341 agggatcaat cggtaaagga gtgggtcgag ggggtagtcc gggaggcctt gatccgggaa
     2401 taccgagagg ctacggagta cgcgccgggc ccgccggata gcgacgagga tcgggcgtgg
     2461 ctcgataatg atctctccgg gctgggggag ttcgaatcct acgaatggga ggaaggggag
     2521 ctggaagaag gtcggcccgt tagctacgag ccgggtgtcg gcgccgtcgt ggagggcggc
     2581 aagcagcgtg gcggaaggta acggggagtc cggcgagcgg ctggagatcg gggacgttgt
     2641 ctccgccctg ttcccgctgc accgtcctgg cgggcgcgag caagaagggt acaggccggc
     2701 cgtggtcgtc ggattgcccg agtttgcggg gactccacgt ttcggagctt tgatcttcac
     2761 tcccatgacg accgaccggg gacaggcatg ggcgcggcgg agcccggagt tgtatccacg
     2821 ctacccggag ggcacggcga atctcaagtc gccttcggtg tgtctgctgg atcaggtaag
     2881 ggctctggat gttggacggg ttcgcgatta tcgcgggacc ttgagtacgg aagagtacag
     2941 acctatagga aaaggcctgg cgaagatgat aagggaccct cgctagatac agagcccgcg
     3001 aaggacaggt cacggagacg acgagtcagg tctgacatat tgttgcccct cttcatcatc
     3061 cagggtgttg ttcaacggag catcaagggt gaggaggtgc ctctcgacta ctgacgcctc
     3121 acgatgcgtc gctttccatg ttctcctcgc tcccaatacg ctccagtatc cgtcgagata
     3181 ctcgcggcga gtctactatt gcttcgagct ccttaacatg ctcttaatcc gtcttccttc
     3241 cagtggagaa tgggagtctg tagactatgt atcattaaat acaaaacgag gtggtggtac
     3301 atgatctcca cggagagaag agtggtgacg tttgtgtggc gatgcacgga ctgcgcgagc
     3361 gtgtacgatg gtgagagcct gctattcttg cacggcgatg ggttcaacag gtattgctcg
     3421 tgcgggggac ggctggtgga ggatgtggag tcacggttgg agcggcactg ccgtctggtc
     3481 ttgccggagc atctgggggg gacgcggctg caccggcagt tggagcggcg gttgtccggg
     3541 gaggactcgg tggaggtctc gaccgggcgc ggggacgacg ggcgcattca tctctttaac
     3601 tgcgccaatt aggggcggct gattgagcgg tagggctccg gcggggagcc gggctctgct
     3661 gttgagccac ctgttgcggg cggtattgtt cggggggctt gcgggttgga tctcttctct
     3721 cctcggaggt gtggtggatg gttgggtcct ttatctgggg tgcgcggcgc tggccggagc
     3781 cgcaggatgg gtgctcgcgc gcccgatgga acggaactcg ttgctggcgg tcctggtcct
     3841 gtggctggtg gcgggtctcc tgtattattc gctagggacg cccgtggagg cggggttgct
     3901 gggggtcagc gtggggctcg gagagtacgt ggagttgggc ggggtagtga tcggtctcgg
     3961 gatcggagcc ctgttgggga gggcggtggc gcgaccgtag cggacggcag atcgggtaga
     4021 gggtcgcgcc ggacgctaga atgcttcgtg tggaagagcg ggagacacga ggcggggcct
     4081 tgcgggttgg gatgcggtgg ggcggtatcg gcgggatcgt cggtttcgcc gcgtcgctgc
     4141 tcgggtcgct cgtcgggata ctggtcgcgg ggttcgtcgg ggtcgcctgc ggccgtcggg
     4201 ccgcggaggc cgatggggga gactcggcgg gggccggggc tctcgcgggg ctcgtgggcg
     4261 gagctgtcgc ggccccggtc tacgtggtcg gcgcgtcggc cggagcgctc gtggcggcga
     4321 ggaccgtcga gatgggtatg gtcgcctcca tgctctcgga gatgctcggc accagcatta
     4381 cctcggaaga agcctggcgg atcttcctca tagccgtcgt cttcgccgcc gcggtccagg
     4441 ccgctgtctt catcctgacg tccgtcgccg cgggagcgtg ggtgaagagg aacagcggac
     4501 aggctccggg cctgtagtag acgaagacgc gccggaccgc cgagagccga acgcttaggg
     4561 cttactgctg cttcgcggct tccgcggctc cctcgccgaa gaccaggccc tcgtcttcgg
     4621 gacgggttac gccggcggcg tccaggcgct ggtctcgctg gtacttgcgg atggcggctt
     4681 tcagggtgcc tagtcccagg gtggcgcact tggggcggga ggcgacgatc tggcgtccaa
     4741 gttgctcgat catctcgttg tagtccatct gcaggacctc gtccatcgtc aggtcgccgt
     4801 cgtggacctg ctccaggatc atggacgaag ccgccatgct gatggtgcag ccctcgcctt
     4861 cgtaggagac gtcctcgatg tgctcttggt ctccggcccc cttcaggtag accgtcacca
     4921 cgtcgccgca gccggggttg ccgcccggca tcgccacgtc ggcgtcctcc agcggaccgc
     4981 gatggcgcgg cttctcgtag tggtccagca ggatctggat ctgtaactgc ctgtccaaca
     5041 cttgcccctc tgtctctaat ctctcgtaac gcccggattc tatcgtaaga atactccggc
     5101 gtgtccggcg gagccgtcca cccatgcagc ccggccccgc gaggcgcata atctccatcc
     5161 gtgagggtcg agcgcgcgga gcatcggagg tgaggggccg ggacatcggc ctgttgctcg
     5221 ccctcggagc gatgtggggc tcatcgttcc tgttcataaa ggtggcgcta ggtgaggtcg
     5281 cgcccgcgtt tatcgtggcc tgccgggtct tcttcggggc gctgacgctt ctcgccgcgg
     5341 tcccgttcct tgaccggatc ttcggcaaga ccccggacgg cctgctccgc agcgtcgccc
     5401 ggctctggcg gccggtgctc gtgctgggcg tcttcaacgc cgccctgccg ttcctggcga
     5461 tctcctgggg tactcagttc ctccccagcg gcaccgccgc catcctcaac tcgaccgtcc
     5521 cgctctttac ggccctgctc gccggactgc tgccgtggat tacggacgag cggctcggag
     5581 tcctcggcgt ggcggggata ctgctcggcg tcgccggggt cgcggtgctg gcgggcgggt
     5641 tcggcgaggg gttttcggcg tctccttcgg aggcacttct gggcgccgga gccatcatga
     5701 cgggctccac gagctacgcc gtcggcgggc tgtacgcgcg ccgggcgatg aagggcgtcc
     5761 cggtgctggt ctccgccgtc ggccagagcg tagcggccct cgtcgtgatc ctgccgttcg
     5821 cggcgctctc gctgccggat accgtgccgt ctctacccgt actcggctcg ctggtggggc
     5881 tcggcgctat cgggaccggg ttctcgctgc tcctgtactt caagctcatc gccaacgtcg
     5941 gggccacccg gacctcgacg gtaacgtacc tggtcccgat gtgggcgctc gtttacggcg
     6001 cgctactgct gggagaggac atcaccgtcc gcgccgtgct gggcctggtc ctgatcctgc
     6061 tcggagtggc gggcgttacc ggcttcgccc gcctcccgcg ccgccgcccg cccgccgctc
     6121 cctgaattgc aacagtttga tacactagcc ccatgagagt cggaatagta gggttgccga
     6181 acgtcggcaa gtccacggtc ttcaatagcc tcacccgcgc gggagccgag gcccagaact
     6241 acccgttcac caccatagac ccgaacctgg gcgtggcggt cgtcccggac gttcgcctgg
     6301 acgcgctggc ggaggtcatg aagagccgga gcgtcgtccc ggcgacggta gacttcgtgg
     6361 acatcgccgg gctggtgcgc ggggcgagcg cgggtgaggg cctgggcaac cagttcctcg
     6421 ggcacatccg ggagtgcgag gccatcgcgc acgtcgtgag gtgcttcgag gacgagaacg
     6481 tcgtccacgt ccacggtggg gtggacccgg tgggggatat cgagacgata cagaccgaac
     6541 tcttgctcgc ggacctcgcc acggtcgagc gtcgcctgga gcggacgagc aaggccgcca
     6601 agagcggtga cgcgaagctc cgggccgagg cggcggagct tgagaagctg cgggagcacc
     6661 tctccgatgg cagcccggcg cgtacttacc cggagagcga ggcgatagcg gaggctatgg
     6721 gcacgctcat cacggccaag ccgacgctct acgtggcgaa cgtggacgag gagtcggtgg
     6781 cggaggggaa ccgctacagc gcggaggtcg agaagctggc ggcgcgcgag ggggcggagg
     6841 tagtgaggct ctcggcgcgg cttgaggcgg aggtcctgga cctgccggag gacgaggcgc
     6901 gcgagtacct ggagatgctc ggcgtcgaga agaccggctt cgaggagttc gtgcgggcgg
     6961 cttactcgct gctcggcctc ataacgttct tcaccgccgg cgaaaaagag agccgggcct
     7021 ggaccgtgaa gaagggcgcg acggtccggc gggcggcagg tgggatccac tccgacatgg
     7081 agcgcggttt tatcgccgcc gaggtcgccg actggcggga cgtcgtctcc gcgggctcct
     7141 ggtcgaagac gaaggaggag gcgaatatgc gcctcgaagg ccgcgactac ctggtccgcg
     7201 acggcgacac cctgctcgtc cgcttcaacg tctaggctgg cggtaacctg tgccgccgtt
     7261 cgtccacccg acaaagcctc tggcagagag cagggtcgcg ctggtaacca ccggcggagt
     7321 acacctcccc gagcagcccc gcttcgacat agacgacccg ctaggtgatt gctcctaccg
     7381 cgagatcccc gtcggggccg aagagcttac ctggacccac gcttactacc ggcccgacga
     7441 aggcgccgac ctcgacgccg tcttcccgct ctggacgctc cggaggctgg ccgcggaggg
     7501 cgtcgtcggc aggccgaacc accgtcactt cagcttcatg ggcgccatcc acgacgccgg
     7561 cccgctcatc gaggagaccg cgcccgaggt cgcgaggaag ctggtcgaag accaggtgga
     7621 cgctgcgctg ctcacacctt cctgaccgct gtgtcaccgg tccgtcgggc tggtaagcga
     7681 ggccatagag gagcagggga taccgaccgt cacgctcgcc atggagcggg gcgtcgaggc
     7741 gccgcgggcc gcgttcgtcc cgttcccgta caactgtccg atgggcgcgc cgggcgacga
     7801 tgagcggcac cgggaggtgg cgatggcggc gttatccctg ctgcacgaga tggatgcgcc
     7861 cggtgaggtt ccgctgccgt tcaagtggcg gaaggggacg ggcgccggac gttaggccct
     7921 cgatatgaag ccgaccgctc ggaccggcga gcggtataat gagtgccaac aaatcctctc
     7981 ccgaaggtga tgcagagtgg ctctcttatt cgttttgttc gtggcgttct tcgtggtcct
     8041 cttcctgttc ctgctgccga tcctgttctc gttgcaggcg gtcgggagct tgttcgtgta
     8101 tccgcggcag ctaaaggcga tgttcgggaa caggatcctg cgccgcaacc acgccctgga
     8161 gcacgcgacc atcgtggtga tgatggagcg ggagccgggc cgaaagctca acggcttctc
     8221 gaccgacgac gggtttttcg tgcagggcgt gcgttcggtg gaggaggtcg agagcgccgc
     8281 gcgcgaggcc ctgcgccggc tgcgggcggg ggagagcaac ctcgccatcc accgcaactg
     8341 cgggacgacc atcgtcgccg ccaacctgct gacggctatc ttgttcctcg gggcgctggg
     8401 cctcggtctg tatatgagct ggccgctgta cctgctcatc ctgggctcca tagcggtcgc
     8461 gctcgcgctg cgggtgccgc tgagcctgct cctgcaacgg tacgttacga ccgacgccga
     8521 ccttaccaac gccgaggtcg ggtgggtaga gcccgcgaag ccagcggacc tgcggagcgg
     8581 catcctcggt gtgctgctcg ccgcctccac ggcgcgggtg cgcgtcttcc acaccgaccc
     8641 ggacgccgtc gagatcatcc gcgacgacgg agcggtagtc cgctagaggt cccgcgccgc
     8701 gccgccggaa cgggcggact ttagcagggc ttctatggcg cgggcgttgc cgaggatctc
     8761 gtcggggccg atggcgggct cccggccctc gcggatggcg gcggcgaact cttccagttc
     8821 ggccaggtat gggtcggctt ctgggaagct ctcggtggcg gtctctccgc cgcgcgtgat
     8881 gttcaggctg ctgccggact tgtccgcccg gaagggggcg tccagggaga tcctgccttc
     8941 cgtacccacg atctccattc gctcccgccg ggcagaggag atgctgcaag agacggtccc
     9001 gactccaccg tcgaactcca gcacgcccgc gacttcccgg tccgccccgt cctccgcgaa
     9061 cctcccgaac gccgccgccc ggcgaggctc cgcgccgaag gccagccgca caccgctcac
     9121 gcagtagcag ccgacatcgc ccagagagcc gccgcccaga gagcccacgc cgcgcacgtc
     9181 ctccggatcg tccagccggt gcccgaactc cgcgatgacc tgccggacct cgccgaccgc
     9241 gccttcggcg agcaactcct tcaggcgcag cgtctgcgga tgaaaccgga acatgaagcc
     9301 ttccatgagc gtcaggcccg accttcgcgc cgcccgcgcg gccttctccg ccgccgccgc
     9361 gtcctgggag aacggcttct cacacaggac gtgctttccg gcgtctatcg ccctgagggt
     9421 ccactccacg tgcatcgagt tcggcagggg gatgtagacg gcgtccacgt ccgggctcgc
     9481 cagcagcgct tcgtagtcct cgaagatccc ggctcgcgga gcaaggtcgc ggacccccgc
     9541 gctcttcccg ccgccgcggg tggctacggc ggagacctcg accgtctccg agcggttcgc
     9601 ggccggaatg atggcgttca gggcgatccg ggcggcgccc aggattccca ggcgcaacgg
     9661 tgggtggttc aagaagctct cctttttcct ttcgcggaag acctccagga ttatattggt
     9721 tggcgcttca ccgcatccag cagggccggg tcccgctctc atcccggtcg ggttcggagg
     9781 ggacgcccca gttctccacc cgtgccgcgt acgcccacgc cgcccggacc gaggggtagg
     9841 catctacggc gctgcgggag acgggcgccg ggaggcatgg cgcttacgca ggaggtcagc
     9901 aggagcgggc ctcctgctag aacatcttgc cgcctggcgc ccggaagagc atcatcgcgc
     9961 tgccggaccc caaacgccgg tacgcgagcc ggtagcggta cttgctcccc ggcggttgct
    10021 gcgatcccgc gtacccgatc ccggagtgtt gtaccctgca ccctcatgga acgcgagcat
    10081 accggcagac atttcaccgt tgcggtcttc gtggtctggg aggggaaggt cctgctccac
    10141 catcaccgca agctcggcat gtggctgccg ccgggcggac atatagagaa agacgagcta
    10201 cccgacgacg cggccctgcg ggaggtgtgg gaggagaccg gggtgcgggt cgcgctcgtg
    10261 ggggagcggc gcgaggacgt agaggacccg gtgcaactcc accggcccgc cggggtgcag
    10321 ctagaggaca tcgggccggg gcatcagcat atagatttga tctacttcgc ccggcccacg
    10381 ggctcgaccg gcatccacga ggacttcagc gtggacagag taggctggta cgcccctgcc
    10441 gactgggacg agatgcaagt caacgccgag gttcgcggct ggtgtgagcg ggcgctactg
    10501 gcttttgagg gcgcgtaacg ccgcgcggac ggctacttct atggccagtt ccacggcccg
    10561 aaccgagaga gccagcgtca tgctgggcgc cgcctcttcc aacgcctgct ctggtaggac
    10621 ggggacgtgg ataaaccctc ccctggtcgg cgagcggcgc gtggcgagca ggtgcatcag
    10681 cgagtagaag acctggttgc acacgaacgt gcccgccgtg ttcgaaatcc gcgccgggat
    10741 cccgcaggcc aggagcgtcg cttcgagctc ccttaccggc agtgtcgacc aataggccgc
    10801 gggtccgtcc gcggcgaccg gctcatccag tggccgccga tccgcgttgt ccggtatccg
    10861 ccgggcgtcg gccgcgttga tcgcgacccg ctctggagtc acctgagaac gccctcgcgc
    10921 ctgcccgagg cagatcacga cctccggcgc ggagtcccgg atcgcgtctt cgagagcccg
    10981 cggcgcacgg gagaagtcgg tcggaaggat gcgggtcgag acctccgcgt tgtcgaaagt
    11041 actgtcctcc agtctcttga cgacttcgcc ggaggcgttg atctcttcgc cgccgaaagg
    11101 atcgaacccc gttaccagta cggtcgcgcg caccttttta cgggtccgag cagtcgcggc
    11161 ggtagccgca gttcggacag atgagcttgc agtggagccc gtacatctcg gcgccgcaga
    11221 tctcgcactc ctcgccggag ggcgcgaggg cttccggggc gtttgtctgt tcgcctgtcc
    11281 gctcgtcgcc caagagatca gactgcgcgc cgggtcttcc aggattgcca gcgtttgcca
    11341 aggatgtccg ccaaatgggc ggtaccggag gtctttacgc tcgtcaggga gagcagccgc
    11401 tgggcctgcc gcagggaagt cccgaggact ccggtgagcg tcttgagcat ggcgaggctc
    11461 tgctgcaact cggagatgcg gatcggcaac tggctcgctc gcgcgtcgag gctggcgacg
    11521 cccctctcaa gctcgccggc gcgcttcgcg aggcgggcga cttcgtcggc cacgtccgtc
    11581 tggaaggcgg cgcgcgctcg acggtatccg agccaggcgc gcagggcgcg gaccgccgag
    11641 atcagggcga gtaccgcgcc gagggagatc aggatgagca agatagcggc catttcgaca
    11701 tctaaagtag cacgagcctc ggcgacttta caaacgcggc gacggtgcta gaatcgctct
    11761 cgcgacgcgg ggtggagcag tctggtagct cgtcgggctc ataacccgaa ggtcgcaggt
    11821 tcaaatcctg cccccgctac taccggcccc ggaactccgg ggcttttctt ctgcctgaat
    11881 taccgatccg cgctcgcctt tatcgccttt gtttggaggc ggttgaggga gggtaggcac
    11941 gaccgtgccg catgaatcct tcctggcgct ctggatgcca ggtatttctg gcggaagacc
    12001 gtagcctcga cgaagggaga gggaatgacc gagaagagtg gaacgcagac gcagcggagc
    12061 ccgttgcaca gcgacaaggg caggaccacc atcagcgacg cggtggttca gcaggtcgcg
    12121 ggcatcgcgg cccaggaggt cgagaaggtc cagatgggcg gcggcacgtc cgcggccgtg
    12181 ggcggcttct tgcaaagcgt taccggaggc ataactggtg gcagcacgtc cggcggcggc
    12241 aactacagca agggcgtcag cgtcgaggtc ggcgaggaag agaccgcgat agacctgacg
    12301 atggctatag agtacggcaa gtccatcccg caggtaacgg aggcggtacg ccggaacgtg
    12361 atcaaccggg tagagagcat ggtcgggctg cgcgtcacgg aggtcaacgt gacagttagc
    12421 gacgtgcaga cacccgagga gcggccgatg ctcgaacagc aggagcagac ggagcagatg
    12481 gcccggcgcg aggagcagcg cgcctagaga ggctacttcg ggcttccgcg aagccggaga
    12541 actaataacg ttgcagagcc ccgccaggcg cggggctctt ttcgtgggcg ctaccggggt
    12601 gcgctacctg gcgccgacgg cggcggccgc gtcgaggcgt ccggcgccgt agtatgggtc
    12661 gcgtccgtcc gggccgaggt ctgtggcggt gctcgtaaga cgccggctta tggcgcgcgg
    12721 gccgcgtccc tgggaggcga gcagaccggc gacgcctgac acgtacgggc tcgagaagct
    12781 ggtgccgctg agggcgctgt agccgccggg cgctgtggag aggatgttga ctccgggggc
    12841 ggcgaggtcg atccagtcgc ctttattgga gaagctcgcc cgcgtaccgg agcggttggt
    12901 ggcggctacg gccaggaccc cgcggtaggc ggcggggtag gagcggacgg tgctgttctc
    12961 gttgccggcg gccgccacga cgaccgcgcc gtggtcctgc gcgtactgga cggcgcgagc
    13021 ttccgcctcg acgaatccgg gagcgccgag ggagaggttg atcaccttcg ctcccctgtc
    13081 ggcggcccag tctatgccct tgatgacggc ggccgagtcg atctcgccgc gccggtccac
    13141 cttcgccatc aggagcttgc agttcgggca ggtccccgca actcccgtcc cattgccggt
    13201 ctccgcggcg gcgacgcccg ccacgtgggt gccgtgacct acggtgtcct cggccacgcc
    13261 gtcaccgttc ccgaagtcgt gttgcgcggc caccttgccg ttaaggtccg ggtgcgacgc
    13321 cttgatgcca ctgtccacca ggccgatcct gacggtgcta tttccttgcg tcctgtccca
    13381 ggcgctcgga gctttgatct gccgcagccc gtactgctcc ccgaacctgg ggtcgtccgg
    13441 cgtcaggctg aactttcgga tgtagttgta gtccaccgcc tcgaccgccg gatctttctc
    13501 caactccctt ttctttcggg ccagagtgtc ttcgcgctcg ctcttggagc taacgtcctt
    13561 gaggccggga aactcgacga gttgcgcgtc cactatcggc aggtcgtcct cgatcctggc
    13621 cctgacctgc tctggcagct tctccatgac ggacgaagaa gcgtcctcct tgtaggtgac
    13681 gatcaactcc ccggcgacgt atgcggctcc gtcgccggtc tcatctacga cccgtcccgt
    13741 gtcggccgac gctcccgacg ccggccacaa catcagcgcg gcgagcgccg atgcgatcaa
    13801 gaccatacga agtcgccatc cgcgctccga aactcccgcg tctctctgca aagaaaaact
    13861 acctgcctct catatagcgc aggaccccct ccagagagcc ccctgaccgt gcacgcccat
    13921 attatcgtgc gtgcgcggag ataactttag cgctccggcg gagtgactta aactgtccgc
    13981 atgaaagcgt ctgaattccc gcagcgcgtg gcccggaccg cggagcgtta cggcatggac
    14041 ctctcccgcc cgctggcgat ggtctccggc ggccctgatt cggtcgcgct cctgcgcgcg
    14101 ctcgtggaac ttggcggcga gccggtggtg ctgcacgtgg atcacggcct gcgcggggat
    14161 gagtcgcgcg aagacgcccg gttcgtgcgg gacctgtgca agaggctcgg cctcgtctgc
    14221 gaaatgcgcg aagcggatct gccggcccgg aacctgcagg aagaagcccg gcgcgagcgt
    14281 taccggatgg ccgaggatgt cgccgccgcg cgcggactct ctgtcatcgt taccggacac
    14341 acctcagacg acgtggccga gacggtgctg ctgaacctgt cgcggggagc cggactgcgc
    14401 ggtctggcgg gcataccccc gatacgcggg cggctggttc ggcccctcat cgaacgccgc
    14461 cgcgcggagg tccttgcata cctgaagtac ctggaccagc cctaccgcac cgatccgacc
    14521 aacctcaccg gcaagtacgc ccgcaaccgc gtgcgcctgg aggtcttgcc cgtccttgag
    14581 gaactgtatc ccggcgccgg tgccaacatc gcgcgcgccg cggacctggt gcgccaggac
    14641 ctggacgctc tggagagtat g
//
LOCUS       DASSMB010000261         8139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1489263, whole genome shotgun sequence.
ACCESSION   DASSMB010000261 DASSMB010000000
VERSION     DASSMB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489263"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFJ72_14895"
     CDS             complement(<1..491)
                     /locus_tag="VFJ72_14895"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HET7480790.1"
                     /translation="MDLTTDAVIIGNPNSGRAGDEGYLERFAKMLREGGLETEVLNTE
                     RPDHATELAALAGDRLVVAAGGDGTVNEVINGLSPGATLGILPLGTANVLARELGLPR
                     NPEAACHRILKGDHSRIDLGVATNREGAERRFACMAGVGFDASVVRTVTPRLKRYLRV
                     LAFP"
     gene            546..941
                     /gene="acpS"
                     /locus_tag="VFJ72_14900"
     CDS             546..941
                     /gene="acpS"
                     /locus_tag="VFJ72_14900"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7480791.1"
                     /translation="MDNFVVPEIGVDVVDCERMKFALERTPRIRKRLFTEAEIAYCEK
                     FRFYERHYAGRWAAKEAVTKALGCGLIQWNGVEVLRRPRRAPTVRIFGKIKEFADRVG
                     VREEDLSISITHSELSAVAVCVVRRNANA"
     gene            942..2444
                     /locus_tag="VFJ72_14905"
     CDS             942..2444
                     /locus_tag="VFJ72_14905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET7480792.1"
                     /translation="MKLYSAPEMSRADETAQELGIPGGVLMERAGAAMAREILDRHDP
                     QRALVVCGGGNNGGDGFVIARELHRAGTEVIVLPTKDGYTGDPETNLRALRNLDVEFA
                     TAEDLEEELAKADLVVDALLGTGFSGAVREKEAALIERMNASGLPVVAVDVPSGVDGS
                     TGEVEGAAVRARLTVCAHATKIGCVISPGREHAGEVAVVDIGIPPEADVEPSLLWTDA
                     DSLRGLTPRTEDPAHKYSAGALLVVAGSRSMTGAAVMSVEGAQRAGCGITFLATPRSA
                     SAALDLKLTETLVYGVQDDEGYMNLKALEDILEHAGRASALVVGPAIGTGDGSRELLE
                     GILREAEAPVLIDADGISLLAGSDALARRDAPTVITPHAGELGRLLGTGAKEVSARRL
                     ESARHAAREHGCCVLLKGSDTLIVEGGRAAVNSTGGVALATAGTGDVLSGVIGALLSR
                     GMDVYDAARTGAWAHGRAAELWLERTDWPAESMVATDLFDYIPPAVREIL"
     gene            2535..3023
                     /locus_tag="VFJ72_14910"
     CDS             2535..3023
                     /locus_tag="VFJ72_14910"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7480793.1"
                     /translation="MPELEQQDIRELKVGEIMHSEWPTLDRDQTVEDVIKVFAESGIS
                     GAPVVEDGRLVGIVTEGDLIFRDADIKAPGFLDILGGMIPLGSWDDYRREAIKSAGVT
                     VGEVMTENVTTVGTDATLAEAATMMAEERIKILPVTEDDRLHGVITRMDILTLHILNP
                     RQ"
     gene            3025..4146
                     /gene="alr"
                     /locus_tag="VFJ72_14915"
     CDS             3025..4146
                     /gene="alr"
                     /locus_tag="VFJ72_14915"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7480794.1"
                     /translation="MAGPEGTILNAATSRTWAEIDLSAIRHNVRSLKARAPRSRLMAV
                     VKADAYGHGAVEVARAAVEAGADFLAVVTVEEGMELRRGGVTVPVLIFTDLAPEKLPL
                     ALEHRLAVTAHSITSARRISARPGLTVHLKVNTGMNRWGVEPDEVGEARKVLGDQLAG
                     VYTHLASADSDEEATLRQLSVFDKVLAAHSFGGALVHAANSAGTLWHPRAHYDCVRPG
                     VAVYGLHPAGDTGDPAQEGMRPAMQIKSYVAAVRRLEPGAGVSYGLTFRARETMYAAT
                     VPVGYAEGYRRGLSGRAEALIRGRRRPLLGRVTMDACVFGVDSSVEEGDEVVLLGEQD
                     GARIGAEELGRWAGTINYEITTGLNPRRVERSYGNVRGT"
     gene            4130..4471
                     /locus_tag="VFJ72_14920"
     CDS             4130..4471
                     /locus_tag="VFJ72_14920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480795.1"
                     /translation="MFEGLNWSNALKRAAMFTGIWLALVYVMHAINPKLFKLGDSGDF
                     LVLALNAVLFFFLYAAFFAFMERRKNRNQKTSSSKKTGTAKNDDEAEPGRFKGQVNPN
                     TSRKKAARRRR"
     gene            4479..5234
                     /locus_tag="VFJ72_14925"
     CDS             4479..5234
                     /locus_tag="VFJ72_14925"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7480796.1"
                     /translation="MKASEALFRALAPPADFDALLDLVPELTLSRELRQGPYHHLDTL
                     DHTQEVVRRVERELEEGRIGARVGEDHVEGLRLAALLHDVAKPVTRGEFEGRVIFVAH
                     DTLGARMIHGICRRLDLSAAVTDAAATLTTLHLKIGFMANPRSDYPPERLVRAAGPFG
                     ESLAVLSWADRLAAQGPRLKDEHIERHRALCGDFLKASRRAGPHPAQDYEDVARRLGL
                     SNCADTGYAASRARLLASRGMGASAALEYVRPR"
     gene            5320..5520
                     /locus_tag="VFJ72_14930"
     CDS             5320..5520
                     /locus_tag="VFJ72_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET7480797.1"
                     /translation="MNIKRAKRDRWIMGVCGGIAHTYGWNPNMVRLATAILAVIIPGP
                     SAVLTLIAYLILGAILPESDEF"
     gene            5537..6169
                     /locus_tag="VFJ72_14935"
     CDS             5537..6169
                     /locus_tag="VFJ72_14935"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7480798.1"
                     /translation="MDFAEAQKEALGCTKCGLCHSRTRVVFGEGPLNAELFIVGEAPG
                     FNEDAEGKPFRGASGMLLDRMLDSLQMGRDEVYLTTLVKCRPPETPPRAPKPGEVSSC
                     RPYLMAQLQAVDPKVVVAMGDLASRVLTGRKEAVSRIRGRAIPLDGRYVFPTLSLNRA
                     LYTPADRALLLSDFSRLPELLDAERPSTYDTLNVPRTPDAEREPLQPGLW"
     gene            6177..6647
                     /gene="tsaE"
                     /locus_tag="VFJ72_14940"
     CDS             6177..6647
                     /gene="tsaE"
                     /locus_tag="VFJ72_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET7480799.1"
                     /translation="MTWEALDQGALESLAARIARVLRPGDVVVLAGEVGAGKTTFVRA
                     AAAALGVQERVTSPTYQLARGYEGTTDGGRRIAVNHLDLYRLEGLEDRDALELDDYLD
                     RRAVTFIEWAEPALGHLAEPTVVRLEHETPETRRLSVEGPVAERIDKDEERRPC"
     gene            6641..7312
                     /gene="tsaB"
                     /locus_tag="VFJ72_14945"
     CDS             6641..7312
                     /gene="tsaB"
                     /locus_tag="VFJ72_14945"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET7480800.1"
                     /translation="MLILTLDASTSTIAVGLARVGDGRRDVLAEMSVSARGASEALLP
                     AVHAVLEIAGGELAEVDLILAGTGPGTFTGIRIAAATSRSLSLGTGAALSANSTLAAL
                     AAPALPVTGDVLAVIDARRGQVFAQRFMDGGPAGEIFCVKPEDLRVDGMPLLAGDGAV
                     RYREVLSRLGNIPPDDSPLHRVSAAGHILSADLAVVAAVDLVPIYVREPDAEVRRDLN
                     PWGRP"
     gene            7309..7752
                     /gene="rimI"
                     /locus_tag="VFJ72_14950"
     CDS             7309..7752
                     /gene="rimI"
                     /locus_tag="VFJ72_14950"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET7480801.1"
                     /translation="MREPLAGPVLRPMCAADLSVVMDIDAESLPRPWSAALWREELAS
                     PFGSYLVLEEEGEVVAHIGTKTVADELHIMTVAVRPRCRRRGYARAMISAAIAASPEA
                     RRVHLEVRPGNAAARALYESLGFVVTGVRPRYYGDEDAILMTLRL"
     gene            7799..>8139
                     /locus_tag="VFJ72_14955"
     CDS             7799..>8139
                     /locus_tag="VFJ72_14955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET7480802.1"
                     /translation="MATLFTKIIEGELPARFVYKDERCVAFMTINPIRAGHVLVVPIE
                     EVDHWLDLDPDLMDHLMRVSRTIGNAIQKSYNPEKVGLMLAGLEVPHVHIHLLPIDRL
                     EDMSFEYAEKDP"
BASE COUNT         1318 a         2713 c         2847 g         1261 t
ORIGIN      
        1 gggaaggcca gcacccgcag gtatcgcttg aggcgcggcg taacggtgcg gaccacgctc
       61 gcgtcgaagc cgacgcccgc catgcaggcg aagcggcgct cggcgccctc acggttggtc
      121 gcgacgccga ggtctatgcg cgagtggtcg cccttcagta tgcggtggca ggccgcttcc
      181 gggttgcgcg gcagccccag ctcccgcgcc agcacgttcg ccgtgccgag cgggagaatc
      241 cccagggtcg cgccggggga gaggccgttg atcacctcgt tcaccgtgcc gtcgccgccg
      301 gccgccacta caaggcggtc tccggcgagg gccgcgagct cggtggcgtg gtccgggcgt
      361 tcggtgttca agacctcggt ctccaggccg ccctcgcgca gcatcttggc gaagcgttcc
      421 aggtagcctt catcccctgc gcgcccagag tttgggttgc cgatgatcac cgcgtcggtc
      481 gtaagatcca tgctgcgtac tatatgccct ttttccggcc gcgtatctcc cgtaagatac
      541 gggccatgga taatttcgtg gtgccggaga taggtgtgga cgtggtggac tgcgaacgga
      601 tgaagttcgc cctggagcgg acgccgcgta tccgcaagcg gctgttcacg gaggcggaga
      661 tcgcctactg cgagaagttc cgcttctacg agcgccacta cgccgggcgc tgggccgcga
      721 aggaggccgt tacaaaggcc ctcggctgcg gcctgatcca gtggaacggc gtcgaggtcc
      781 tgcgccgccc gcgccgcgcg ccgaccgtga ggatcttcgg gaagatcaag gaattcgccg
      841 acagggtcgg cgtccgcgag gaagacctct ccatctcgat cacccactcg gaactctccg
      901 ccgtcgccgt ctgcgtcgtg cgacgtaacg ctaacgctta gatgaagctt tactccgcgc
      961 cggagatgtc ccgcgccgac gagaccgcgc aggagttggg cattccaggc ggcgtcctca
     1021 tggaacgggc cggcgccgcg atggctcggg agatactcga ccgccacgat ccacagcggg
     1081 cgctcgtcgt ctgcggcggc ggcaacaacg gcggtgacgg cttcgtaatc gcccgcgagc
     1141 tacaccgcgc cggaacagag gtaatcgtgc tccccacgaa ggacgggtac accggcgacc
     1201 cggagaccaa cctgcgggcg ttgcgcaacc tggacgtcga gttcgcgacc gccgaagacc
     1261 tggaggaaga gctggcgaaa gcggacctcg tcgtggacgc gctgctcggg accggcttct
     1321 caggggccgt gcgggagaag gaggcggctc tgatcgagcg gatgaatgcc tcggggcttc
     1381 cggtcgtggc ggtggacgtg ccttcgggcg tggacggctc gaccggggag gtcgagggcg
     1441 ctgcggttcg ggcgcggctc acggtctgcg cgcacgcgac gaagataggg tgtgtgatct
     1501 cccccggccg cgaacacgcc ggggaggtcg ccgtcgtgga catcggcatc ccgcccgagg
     1561 ccgacgtgga accctcgctc ctgtggacgg acgcggactc gctccgtggc ctcactccac
     1621 gcaccgaaga ccccgcccac aagtactccg ccggggcttt gctcgtagtc gcgggctccc
     1681 gcagcatgac cggagccgcc gtgatgagcg tggaaggcgc gcagcgcgcc gggtgcggca
     1741 taaccttcct cgccacgcca cggagcgcct cagccgccct cgacctgaag ctcacggaga
     1801 cactcgtcta cggcgtgcag gacgatgaag gctacatgaa cctcaaagct ctggaggaca
     1861 tcctggagca cgccgggcgg gcgtcggcgc tcgttgtcgg tccggccatc gggacgggcg
     1921 acggcagccg agagttgctg gagggaatcc tgcgcgaggc ggaagcgccg gttttgatcg
     1981 acgccgatgg gatctcgctc ctcgccggct cggacgctct cgccaggcgc gacgccccga
     2041 ccgtcataac gccgcatgct ggagagctgg gccggctgct gggaacgggc gcgaaggagg
     2101 tctccgcgcg caggctcgaa tctgcccgtc atgcggcgcg ggagcacggg tgctgcgtgc
     2161 tgctcaaggg ctccgacacg ctaatcgtcg agggcgggcg ggcggcggtc aactcgaccg
     2221 gcggcgtggc gctggctacc gctgggacgg gggatgtgct ctccggggtg atcggggcgt
     2281 tgctctcacg cggcatggac gtctacgatg cggcgcggac cggggcctgg gcgcacggcc
     2341 gggcggcgga gttgtggctg gagcggacgg actggcccgc cgagagcatg gtagcgacgg
     2401 atctcttcga ctacatcccg ccggcggtgc gcgagatcct gtgaggtcca gttccgggag
     2461 atgcggcggg gcggtcggct gaggtagtat ccagggcaga agacctgggt agttggacga
     2521 gatcggttcc gggggtgcca gagttggagc agcaagacat aagggagtta aaggtcgggg
     2581 agatcatgca ctccgaatgg cccacgctcg accgagatca gacggtggag gatgtgatca
     2641 aggtcttcgc cgaatcgggt atctccgggg cgccggtcgt ggaggacggc aggctcgtcg
     2701 ggatagtgac ggaaggagac ctgatcttcc gggacgcgga catcaaggcg ccgggatttc
     2761 tggacatcct gggtggtatg atcccgctcg ggagctggga tgattaccgg cgggaggcga
     2821 tcaagagcgc cggcgtcacg gtgggcgagg tgatgaccga gaacgttacc acggtcggca
     2881 ccgacgccac tctcgccgag gcggcgacca tgatggccga ggagcggatc aagatactgc
     2941 ccgtcacgga ggacgaccgg ctccacggtg tcatcacccg catggacatc ctcaccctcc
     3001 acatcctcaa cccgcgccag tagcttggcc gggccggaag gcacgatcct caacgctgcc
     3061 acgagccgca cctgggccga gatagacctc tcggcgatcc ggcacaacgt ccgctccctg
     3121 aaggcccgcg ccccgcgctc gcgcctgatg gccgtggtaa aggccgacgc ctacggtcac
     3181 ggcgcggtcg aggtggcccg cgcggccgtc gaggcgggcg ccgacttcct ggccgtcgtc
     3241 acggtggagg aggggatgga gctccggcgg ggcggcgtca cggttcctgt actgatcttc
     3301 accgacctcg cgccggagaa gctgccgctc gcgctggagc atcgcctggc ggtgacggcg
     3361 cactccatca cgagcgcgcg ccggatctcg gcgcgaccag gtctcaccgt tcacctgaag
     3421 gtcaataccg gcatgaaccg ctggggcgtc gagccggatg aggtggggga ggcgcgaaag
     3481 gtcctcgggg atcagctcgc aggcgtctac acgcacctgg cctccgccga ctcggacgaa
     3541 gaggctaccc ttcgccagct ctcggtcttc gacaaggtcc tcgctgcgca ctcattcggc
     3601 ggtgccctgg tccacgcggc gaactcggcc ggcacgctct ggcaccctcg cgcccactac
     3661 gattgtgtgc gtcccggcgt ggccgtctac gggctgcatc cggcaggcga taccggagac
     3721 cctgcacagg agggaatgcg tccggcgatg cagataaaga gctacgtcgc ggcggtgcgg
     3781 cgtctggagc cgggcgccgg ggtctcctac gggctcacgt tccgcgcccg cgagacgatg
     3841 tacgcggcga cggtcccggt tggttatgcg gagggctacc ggcgggggct ctcgggacgg
     3901 gcggaggccc tgatccgggg caggaggcgt ccgctgctcg gacgggttac gatggatgcg
     3961 tgcgtcttcg gggtggattc ctcggtggag gagggagacg aggtcgtgct gctcggggag
     4021 caggacggcg cgcggatcgg ggccgaagag ctcgggcgct gggcgggcac gataaactat
     4081 gagataacta ccgggctgaa cccccggcgc gtcgagagga gctacggaaa tgttcgaggg
     4141 acttaactgg tcaaacgccc tgaaaagggc cgctatgttc accggcatct ggctcgctct
     4201 cgtgtacgtg atgcacgcca tcaacccgaa gctcttcaag ctgggcgaca gcggcgactt
     4261 cctcgtcctc gccctgaacg ccgtcctgtt cttcttcctc tacgccgcct tcttcgcctt
     4321 catggagcgg cgtaagaacc gcaatcagaa gacctcgtcc tccaagaaga ccggaaccgc
     4381 gaagaacgat gatgaagccg agcccggacg attcaagggg caggtcaacc cgaacaccag
     4441 ccgcaaaaag gccgcccgcc gccggaggta ggcacgccgt gaaggcctcc gaggcgctgt
     4501 tccgggcgct cgctcccccg gcggacttcg acgcgctact ggacctggtg ccggagctga
     4561 cgctctcaag ggagttgcgg caggggccgt accatcacct ggacaccctg gatcacaccc
     4621 aggaggtcgt ccggcgcgtg gagcgcgagc ttgaggaagg acgcatcggg gcgcgtgtcg
     4681 gggaagatca cgtcgaaggt ctgcggctcg cggctctcct gcatgacgtc gccaagcccg
     4741 tcacccgcgg cgagttcgag gggcgtgtga tcttcgtcgc ccacgacact ctgggggccc
     4801 gcatgatcca cggcatctgc cgccgcctgg acctctccgc cgccgttacg gacgccgccg
     4861 ccacgctgac aaccctgcac ctgaagatag gctttatggc gaacccccgc tcggactacc
     4921 ctcccgaacg tctcgtccgc gccgccggac ctttcggcga gtcgctcgcc gtcctctcct
     4981 gggccgaccg gctcgccgcg cagggcccgc gcctcaagga cgagcacata gagcgccacc
     5041 gcgcgctctg cggggacttc ctcaaagcca gccgacgcgc cgggccgcac cccgcgcagg
     5101 actacgagga cgtcgcccgc cggctgggtc tctcgaactg cgcggacacc gggtacgccg
     5161 cgagccgggc gcggctcctc gcctcccgcg gcatgggcgc gagcgcggcc ctcgaatacg
     5221 tccgcccgcg ataggattcg ggaaagggag agaaaagccg gtcatggtag aatcgcggcg
     5281 tcggcggaac gattttgtgg agaccggggg aggttgatct tgaacatcaa gcgcgccaag
     5341 agagatcgct ggatcatggg agtctgcggg ggtatcgccc acacgtatgg atggaacccg
     5401 aacatggtac ggctcgccac ggccatcctg gcggtcatca tccccggacc gagcgcggtc
     5461 ctgaccctga tcgcctacct catcctcggc gcaatcctcc ccgagagcga cgagttctag
     5521 ggagcattct ccagtcttgg acttcgcgga ggcgcagaaa gaggcgctcg gctgcacgaa
     5581 gtgcggcctg tgccactcca ggacgcgggt cgtcttcggc gaagggccgc tgaacgcgga
     5641 gctattcatc gtcggggagg cgccgggatt taacgaggac gccgaaggga agcccttccg
     5701 gggcgcgtcc gggatgctgc tcgaccggat gctcgattcg ttgcagatgg ggcgcgacga
     5761 agtctacctg accacgctgg tcaagtgccg cccgcccgag accccgccgc gcgctcccaa
     5821 gcccggcgag gtcagctcct gccggcccta cctgatggcg cagcttcagg cggtcgaccc
     5881 gaaggtcgtg gtggcgatgg gagacctggc gagccgcgtc ctgaccgggc gcaaggaggc
     5941 cgtctcgcgc atccgggggc gggccatacc gctcgacgga cgctacgtct tccctacgct
     6001 ctcactcaac cgggcgctct acacccccgc cgaccgggcg cttttgctct cggacttctc
     6061 ccgcctcccg gagttgctgg acgccgaacg tccctccacc tacgataccc tcaatgtccc
     6121 ccgcaccccc gacgccgagc gcgaacccct ccagcccgga ctctggtaga ggacccatga
     6181 cctgggaagc cctggaccag ggcgctctgg aatcccttgc cgcgcggatc gccagggtct
     6241 tgcggccggg cgacgtcgtg gttctggcgg gagaggtggg agcgggcaag acgaccttcg
     6301 tccgggcggc tgctgccgct ctgggcgtac aggagcgtgt aaccagcccg acctaccagc
     6361 tcgcccgagg ctacgagggc accacggatg gcgggcgccg gatagcggtg aaccacctcg
     6421 acctctaccg gctcgaaggt ctcgaagacc gcgacgcgct ggagctggac gactacctgg
     6481 accgacgggc cgtgacgttt atcgagtggg ccgaacccgc gctcggacat ctggccgagc
     6541 cgaccgtcgt ccgcctagag cacgagaccc ccgagacgcg ccggttgagc gtggagggtc
     6601 cggtcgcgga gcggatcgat aaagacgagg agcgccgccc gtgctgatcc tgaccctgga
     6661 cgcctcgacc tcgaccatag ccgtcggcct cgcccgcgtg ggggacgggc ggcgcgacgt
     6721 gctggccgag atgtcggtct ccgcgcgcgg cgcctccgag gccctgctac cggcggtcca
     6781 cgccgtcctg gagatcgccg ggggggagct tgcggaggtg gatctcatcc tggccgggac
     6841 cgggcctggc accttcaccg gcatccgcat cgccgccgcg acctcccgct cgctctcgct
     6901 agggaccggg gccgcgctct cggccaactc caccctcgct gcgctggccg cgcccgccct
     6961 tccggtgacg ggagacgtcc tggcggtcat agacgctcgc cgggggcagg tcttcgctca
     7021 acgctttatg gatggcggtc cggcggggga gatcttctgc gtaaaaccgg aggacttgag
     7081 ggtggacggc atgcctctgc tcgccggaga cggggcggtc cgttaccggg aagtcctctc
     7141 ccgtctgggc aacatcccgc cggacgactc gccgttgcac cgggtaagcg cggcggggca
     7201 tatcctctcg gcggacctcg cggtagtggc cgcggtagac ctcgtgccga tctacgtgcg
     7261 cgagccggac gccgaggttc gccgggacct caacccctgg ggccgaccgt gagggagccg
     7321 ctcgccgggc cggtgctgcg gccgatgtgc gccgcagacc tctccgtggt aatggacatc
     7381 gacgccgaga gtctcccgcg cccgtggagc gcggcgctct ggcgtgaaga gcttgcgagc
     7441 ccgttcggtt cttacctggt tctggaggag gaaggagagg tcgtcgccca catcgggacg
     7501 aagaccgtgg cggacgaact gcacatcatg acggtcgccg ttcgtccccg gtgccgccgc
     7561 cggggttacg cgcgcgccat gatctccgcc gccatcgccg ccagtcccga ggcgcggcgc
     7621 gtccacctgg aggtgcggcc cggcaacgcc gccgcccgcg ccctctacga gtcattgggc
     7681 ttcgtcgtca ccggcgtgcg cccccgctac tacggcgacg aagacgccat cttgatgacc
     7741 ctgcgcctct gaggtgctac cctcgccctg acgagcaggg acggcaggga ggtcggcatt
     7801 ggctacgttg ttcacgaaga taatcgaggg agagctaccg gcgcgcttcg tctacaagga
     7861 cgagcggtgc gtcgcgttca tgaccataaa ccccatccgg gcgggccacg tcctcgtcgt
     7921 gcccatcgag gaagtggacc actggctaga cctcgacccc gacctgatgg accatctgat
     7981 gcgagtctcc cgcaccatcg gcaacgctat ccagaaatcg tacaaccccg agaaggtcgg
     8041 cctgatgctg gcgggcctcg aagtgccgca cgtccacatc cacctcctgc ccatagaccg
     8101 gctcgaagac atgagcttcg agtacgccga gaaggaccc
//
LOCUS       DASSMB010000262        23811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1352154, whole genome shotgun sequence.
ACCESSION   DASSMB010000262 DASSMB010000000
VERSION     DASSMB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 23811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23811
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352154"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VFJ72_14960"
     CDS             complement(<1..292)
                     /locus_tag="VFJ72_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480803.1"
                     /translation="MLVRRGLLSSFLGGLLRSQFLSRSLFGSSLLGGGLFGGSLLGGG
                     LFGGLLSSGLFYRGLFGGVTGLLGGGLSLLRPLLGSGALIGFLLSSLLLGEFC"
     gene            437..736
                     /locus_tag="VFJ72_14965"
     CDS             437..736
                     /locus_tag="VFJ72_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET7480804.1"
                     /translation="MPYVYGGASRSGVDCSGLTMLVFQKFGISLPHSAAAQYGYGSTG
                     SGTAGDLVFWDEGGGISHVGIATGSGTTIHAPYPGTVVREEGIWSNGYVGAKTLL"
     gene            878..1828
                     /locus_tag="VFJ72_14970"
     CDS             878..1828
                     /locus_tag="VFJ72_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HET7480805.1"
                     /translation="MSLSLLQAFGFGFAAIVLVFGAIVFVSTILSAVKIVNEYERGVI
                     FRLGRVLGAKGPGLFLLAPIFDRMVKVDLRTVTMDVPPQDVITRDNVPARVNAVVYFR
                     VVDPNKSVIEVENYVLATSQISQTTLRSVLGQKDLDDLLTNREAINDELQKIIDEQTD
                     PWGIKVSVVEVKDVEIPQQMQRAMARQAESERERRAKIIAAEGEYQASERLRQAADRL
                     ESPTALQLRLFQTLGEVANQQNSTIILPVPMDLFRPYLNAHESPANGRTASRAERQTS
                     DGDRQPERLYEKTVEETRAGMPQAPAEEPPDREFPGASGI"
     gene            1980..2420
                     /locus_tag="VFJ72_14975"
     CDS             1980..2420
                     /locus_tag="VFJ72_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7480806.1"
                     /translation="MGHETTETKRGEEIGFSEPIALRIPSRSEYVLLARLVVSQVGQL
                     ASFEPEDIYDLKLAVTEAATNVIRHAAVDHFDIEYRVSRHAVEITVTDAGGGFDADGL
                     THAPDERGGFGLAVIQNLVDEVVLDTAAGGGTRLKMIRRASATV"
     gene            2546..3796
                     /locus_tag="VFJ72_14980"
     CDS             2546..3796
                     /locus_tag="VFJ72_14980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7480807.1"
                     /translation="MLQRVNPGTKSLADYRSIIRRELYDEIRDLSARLDGARVLHVNA
                     TSFGGGVAEILYTLVPLMRDVGLQADWGVMFGAEPFFNVTKGFHNALQGADYDLTVEA
                     RAIYEEYNRQSAAALVEAGEEWDLMFIHDPQPALLKHFSGGLSEKTKWIWRCHIDTST
                     PNKQVLNYLAPYIEDYDAQIYTMQEYTPPGVELPNLTLIPPAIDPLSPKNMALSPDDA
                     RYIANQFGVDTSRPFILQVSRFDPWKDPLGVIDVYRAVKEEVPETQLVLIGSMAHDDP
                     EGWDYWYKTVNYAAGDQDIFLFSNLTNVGAIEVNAFQSLADVVIQKSIREGFGLVVSE
                     ALWKGRPMVAGRVGGIPMQVTAGGGILVDTVPEAAAACAKLLKDSEFAAEMGRRGKEH
                     VREHYLTPRLLRDDLALFAKLLGV"
     gene            complement(3864..4694)
                     /locus_tag="VFJ72_14985"
     CDS             complement(3864..4694)
                     /locus_tag="VFJ72_14985"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET7480808.1"
                     /translation="MFTSARMHRRRMRLAIAIVMLALLATAGYALLRDNPSPLVFSTG
                     EAAAVKDAPAPPKAKQDKKAKAPEKQIAKGPPLPAPAESCDDLRVLVDHKHPLPYGYV
                     PQDLVSLSAFGVPVVDGDQLLRREAAGQLQLLMAAAAADGEDLVIASSYRSYTDQQAS
                     HDKWANFYGDPNAGGMSALPGHSQHQLGTAVDFTNSAAGYKIWQPFGRTSGSTWLKKN
                     AGEYGYVLSYPEGLEAETGYQWEPWHYRYIGVRNAERLKESGMSLQRFLTEEGVLPRC
                     "
     gene            complement(5241..5441)
                     /locus_tag="VFJ72_14990"
     CDS             complement(5241..5441)
                     /locus_tag="VFJ72_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480809.1"
                     /translation="MATWTNKQWGALIGGLVIFLIAVYGIGVAALVVLFAIIGYFIGK
                     FLDGEIEVDDIRDRAQGRRSGM"
     gene            complement(5460..6020)
                     /locus_tag="VFJ72_14995"
     CDS             complement(5460..6020)
                     /locus_tag="VFJ72_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480810.1"
                     /translation="MNAFNRFIMLIIALLLVAIPVLLLLVAFGALSARLIDQYTNYSA
                     AVSTLGNLSPSNLSGNAGIIIGIIGLLVALVALLLLLRELTFGRRVARSTLIDDTPGK
                     ETTITARAVKTLAEGAAWEVGAVSPSVSLTSKDRTYLVSCKIKAPRTSNYTELATRTR
                     ENIRQVLEGQSVPVKDVEVTVQGTVV"
     gene            complement(6017..6583)
                     /gene="amaP"
                     /locus_tag="VFJ72_15000"
     CDS             complement(6017..6583)
                     /gene="amaP"
                     /locus_tag="VFJ72_15000"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET7480811.1"
                     /translation="MRIFNRIVMVLLFCGLFLLGVYMVAYGFNLFGYQLSDLPVSSFG
                     QGVSDFVSGVENGNLPVLMTITLALIALLGLFLFIAELKPRTPRRVRMQKGTYITRGT
                     VEGEVEHATEETPNVLDSSVRVKARRRPGAQIDLAANVRRGDEVNTIKSDLQNRVQQH
                     LASSGIPVNRLKIRMVESDPRETKTRVQ"
     gene            complement(6570..6872)
                     /locus_tag="VFJ72_15005"
     CDS             complement(6570..6872)
                     /locus_tag="VFJ72_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480812.1"
                     /translation="MADELQLARVASEAALATEGVHSMGVGRYAEAATYEGREKVTGV
                     VVKPDEVEIHIVARYPLPSPIPEIASNIMARVAPQVGSRTTRIVVEDLEMGADENL"
     gene            complement(6890..7414)
                     /locus_tag="VFJ72_15010"
     CDS             complement(6890..7414)
                     /locus_tag="VFJ72_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7480813.1"
                     /translation="MTEQRTGSTGGSGSGSASSSSSSPLQTDRGNTTIQDTVVSKIAG
                     IAAQEVEGIRMGGGTSQAVSGLLGSITGGSGSGSQTQGVSVEVGQEEAAVDLTLTAAY
                     GKSIPQLAEAVRRNVVNRVESLVGLRVTEVNITVSNIFFPEQEAEQQRQKELEQQQRE
                     QQRQQEAEQQQRVQ"
     gene            complement(7481..7678)
                     /locus_tag="VFJ72_15015"
     CDS             complement(7481..7678)
                     /locus_tag="VFJ72_15015"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7480814.1"
                     /translation="MPGPDPGGIIVTILIGIAGSFVGGFLLKPLFGGGGANLSLGSVL
                     MATLGAIILLAVYRLITRRAA"
     gene            complement(7988..8560)
                     /locus_tag="VFJ72_15020"
     CDS             complement(7988..8560)
                     /locus_tag="VFJ72_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480815.1"
                     /translation="MLVRLFIVMVFTFCGYLAVANLSFGLVGDPKVITAEQINRGKLP
                     AGIEQGDYVEVRGIPEFGSETRVLGTPESEVGVVSRYTSANYFYFRLKNTGDNLLIQK
                     AQTPPDFQNPPHVFRGKLATVGTVIFHDTTQAGLKQARLPHKETIPVIQSSDTPEYYR
                     QLFPAYLIIIGLWILSVIYLIWRKNKPFLD"
     gene            8859..9527
                     /locus_tag="VFJ72_15025"
     CDS             8859..9527
                     /locus_tag="VFJ72_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480816.1"
                     /translation="MERGREEPKSGSEYTVIGVISDGAELNQAVTEVRELGVGRDDLT
                     VILKRQDPSEPEPFPEGTRYIVIPNDRRGLEVPIGFAIAFVVFGIFFALSTPAIGIPT
                     LMVFVSLASILVAGSLTRVGVSPILTEMEAPREEAGTWNDEFEMGKVLIFATTRERRL
                     IRPIREAMQRGGAMYYIVNRRLEPRAVHQAILKRAGSYADSITGGPKSRVGETRDTAS
                     EGTR"
     gene            9539..10891
                     /locus_tag="VFJ72_15030"
     CDS             9539..10891
                     /locus_tag="VFJ72_15030"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7480817.1"
                     /translation="MGTPTISTLQFLAQLDLSNTFGFLGGARVITGLSMLVHMFFAQI
                     FVGFALAAPVLQAWGARTGSPRMDRLAHSMARFNVLTFSTGATFAVLFIVLLVGFYPR
                     VTASLFTHFFYFLAIAMLSMVLSLWGMYSYYYKWDRYSVLKKKRHIALGFTMGFFIWI
                     WMLIMTGIDTYMTTGGGPNSTPLNGNSIESLGVAFQGIFNPMFVEMLLHRTFANLSWP
                     AFALAAWAAFMYIRSKTQEDRAFYDWSTSVGLTWGTGFLIVQPLVGFAIVYAMKLAVP
                     QSPAEETARGGAYERLTSGDTSNILYLNLLMVVGLFVLSNIAMYMGAGRHPDRGGRIP
                     IRFFGLVAAVAGLYSISPLASFPYESPFFYLRYIAILIMLLATFGAFITYLRGRMRFR
                     YGSPGAAYRVTLLALGVLAAVVALDMGLMKSNSRVPYTVYNQPDYTVESGPPPSGFGR
                     "
     gene            10929..11627
                     /locus_tag="VFJ72_15035"
     CDS             10929..11627
                     /locus_tag="VFJ72_15035"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET7480818.1"
                     /translation="MTELQKDPITRSDFLGFGFIGAIMGAILTIPPVAFVLGPVIKTD
                     VLGKSDISKGWQEVGKVSEIKAGEPKDFTIEFPLTQSYGDKSIQEKAPSSPRSNKQFT
                     LRNAVWASWKTKYKPNGSQGKVSRPSFLDQKTEGFSQSEIKEIEKNLNVLSNSCAHLG
                     CPVRWVITEGEGEFLCPCHGGLYDINGDYQGGPPPRGMYHYKTWEVRENGKLYVKHGY
                     IIQDRPDATQPYVV"
     gene            11670..12494
                     /locus_tag="VFJ72_15040"
     CDS             11670..12494
                     /locus_tag="VFJ72_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET7480819.1"
                     /translation="MIGRLRSQTSSASKVIVDWMEHRAGIVTAMEHFLYEPVPKRGAW
                     LYTLGSAVLFLISLQFLTGILLLFYYVPTTDHAWNSVYYIMNEAYFGTLIRGIHYWSA
                     NFLMAMIGLHMAQVFFSGGYKAPRELNWIVGVVLLLLVIGLAFTGYLLRWDQDGFWAS
                     VVGIKIGSYSPFVGAYITHFLLGGDAVGPATLSRFFAIHVWLLPAAIAPFIGIHLYLL
                     RRHGVFGSEFEYSERIAKMHEHQRMEEYARYELSDDEHEEEDGYEGNGRGRYDGGR"
     gene            12577..13062
                     /locus_tag="VFJ72_15045"
     CDS             12577..13062
                     /locus_tag="VFJ72_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochromesubunit B of the bc complex-like
                     protein"
                     /protein_id="HET7480820.1"
                     /translation="MYAPATGKAKPVEEEVITEENVFDRPDETIGFFPGQVLKDAYVS
                     FFLLLACCALSYIAPAPLTAPANTATITYVPRPEWFFFFYEQMLMFFPGYALVSFGAV
                     VVPTIFIVTLFALPWLDRGPNFSSLRRPFTAVVGILIIVVVLLNMLLAVSRIMNFPSG
                     G"
     gene            13075..14424
                     /locus_tag="VFJ72_15050"
     CDS             13075..14424
                     /locus_tag="VFJ72_15050"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7480821.1"
                     /translation="MPIGDLQVPVIGKNVVIASLVQTHILFAAFIIGAVLIAATSEYL
                     GLVTKNPRYERFARNLARFVVLLFASGAALAITFVLALVTLFPVFFSYLQNVFFWVFL
                     VEAFMFLGQIIIVYAWYNVWDKLAYRKSLHVVFGFVAGFMGLMAMTMIDGVASFMLTP
                     AETEVGNVARTFLNQTNVPLNMHRFVGNFSYAGFLIAGWGAWRYLRSTREDDREYYDW
                     MGHFGLIWGFGFLIIQPVIGYGYLKGIRESSPDAFNTLMLGDKAWLFNFLALWLAIMS
                     IASTAYFVHKLRFAVKPMETLRKLSVGALGFTALFTVLNVIPANTSFIPQIGLIFLGG
                     EKTKIPLGSMYPYKYIGLIGMMLVGIFVVALYLRAAASGFHWGRASRWSQFALLACSV
                     TVVLTMVTMGYTRETARRVDYTPAGNGGGYLIYKCITLDQKPTPSTCPTKGLGGGGE"
     gene            14424..14561
                     /locus_tag="VFJ72_15055"
     CDS             14424..14561
                     /locus_tag="VFJ72_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480822.1"
                     /translation="MDLALLLQDGATSGIGIFRFFAGFILVLCFYLGALGFTYWISKD
                     E"
     gene            complement(14684..15514)
                     /locus_tag="VFJ72_15060"
     CDS             complement(14684..15514)
                     /locus_tag="VFJ72_15060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7480823.1"
                     /translation="MSDKLSLLKRSTTGILSHDPAHGSLTGYDVFVSAHSPASRTAII
                     TGASSGIGEATARLLAQNGYNVVLAARRADRIEALATALGQRALAVPTDITDPAACED
                     LVTRAVERFGALDLLVNNAGIGLYSTIADADPEDWRRMFDVNVLGALCCTRAAIRHMI
                     PHGSGDVVFISSVAGRRVPNAYGTVYAATKHALSAITGGLRMEVEGKNIRVISVEPGL
                     VRTEFPNNSYSSAAEYYAQKEYAPLEAGDVAEAVLYAVSQPRSVSVDEVLLRPTDQIN
                     "
     gene            15504..16157
                     /locus_tag="VFJ72_15065"
     CDS             15504..16157
                     /locus_tag="VFJ72_15065"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET7480824.1"
                     /translation="MSDIPLFPLNVVLMPGAPLPLHIFEDRYKQMVDECLESESEFGM
                     VLADEAGTREVGCTARIVELVERYEDGRMVILVEGSRRFKLNSVMTGKPYYVGEVEYI
                     EEEPEEDVTELAEECKALLERVVEAATEGSVAIEIEPPYRNLSFAIAGRIEFDVETRQ
                     QILELMTERARLEKVKELLSSAAERLERDRQAREKAQTNGHLRPNYRPGEKPEESPS"
     gene            complement(16154..17161)
                     /locus_tag="VFJ72_15070"
     CDS             complement(16154..17161)
                     /locus_tag="VFJ72_15070"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7480825.1"
                     /translation="MLIALLQNNAEETTSSIRAAADKAGDAAKGASKGTFDVFKGIND
                     FAAASFKYLTGAEFISNIIASVVVILLGIFAYRILTRGVPRVLRWRRSHRDVLDAEAV
                     ARIKRQDTAITLIRNALRYVVFVVIILFVLSIFVRNPLPATAGATILAAVLGFGAQSF
                     LRDVIAGFSIIFEGQYSVGDFIQIKPQDISGIVEELGLRMTKIRSLSGEVSYIPNGTM
                     QSVTNFVSGQQRFTVEVQLSDPEAVERVLGSLDEASNLYLTPPRLVGQEENEGRIRLR
                     IFAGVLPSMAWLVEENLTGRIKAAAGEDALASEPLIYKVDQANLRRIRDLIPQEVDKE
                     D"
     gene            complement(17306..17878)
                     /locus_tag="VFJ72_15075"
     CDS             complement(17306..17878)
                     /locus_tag="VFJ72_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480826.1"
                     /translation="MILVLIFTLAVPALAQDPGSAPTGVADQYGGLADIEETGDAVTV
                     EGTVREAGITSYQYGTHVLLGESGNLLFALRSETVTLDDYVGQRVTVHGARVSGYQNG
                     AVEGGPDLLDVTRVDNGSATHPGDQSDTDTSGADSSGTGDSTPSHQDTGDAAAAVSVL
                     PDTGGIPVSSLLAINAALISGAVLARRIVG"
     gene            18054..19673
                     /locus_tag="VFJ72_15080"
     CDS             18054..19673
                     /locus_tag="VFJ72_15080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7480827.1"
                     /translation="MFRSRIASSGARWWWTLAAGAGIAFLTFVVMFVMIDVYLTIATM
                     VSRGDVDQSQVQTFADFASVWALPVLFLVLTAGATAWMARRFGVTSLWFGVLVGAISA
                     IGFQLIGLLFSPPETRELIVYPLCGVAGGLLGIFLGRISIAGREALYRASRDVGAASS
                     ADEVASAIGRHLAGSNVEQVSLWSVASREKDEDLRLDLTGSWSARTEALWPSGASLDS
                     ELIPDLVGMRRQSPRVVRGREVPESERANWERRGVRAVLLVPLSASGEGPDGLLAVAS
                     RGPRFSAGAVRAYLTVGAQAALALENLRLVRGARHSGMMDERRRLAHEIHDTLIQGFA
                     SIVMNLEAAEGKSGDDPARAYRHLREARRTARESMDEARRMVWALRPEALEGASLSEA
                     FSRLAERWSESNGVEAVVTVTGSPRRLAPETEVTLLRVAQEALANVRKHAAASRVVLT
                     LSYMEDRVTLDVQDDGVGFETAASRNGSGSSVNGDGLGGFGLRAMRERVEQNGGRLLV
                     ESEPGRGTALVAELPLAGGVSQGARASQEAR"
     gene            19670..20302
                     /locus_tag="VFJ72_15085"
     CDS             19670..20302
                     /locus_tag="VFJ72_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480828.1"
                     /translation="MMGRIKLLIADDHPVVRMGLRGMLDGQEDFEVVGEVENGAQAVE
                     ATERLRPDVVLMDLRMPEMDGVAATTRIVERTPEARVLVLTTSDSGADILRAVEIGAT
                     GYILKDAPREELFRAVRAAAEGKPLLAPDVAAHLMNRVRWPSDEALSSREIEVLELVA
                     QGTSNRNIAGKLWISEATVKSHLLHIYGKLGASDRASAVSVAMKRGILRS"
     gene            complement(20317..21366)
                     /locus_tag="VFJ72_15090"
     CDS             complement(20317..21366)
                     /locus_tag="VFJ72_15090"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET7480829.1"
                     /translation="MVIFGGRSGEHDVSLASARAITDALEKSGRYEVVPVGITREGRW
                     ISSGDPMRELESASKALDSANGSESPISDSPASISEKLPANLGSVDVVFPVLHGPYGE
                     DGTIQGMLELAGVPYVGSGVLASAASMDKITMKKIFAHHGLPQVEWLGLTRTNWERDS
                     KSILRQIEDSIGYPCFVKPANLGSSVGISRATDAGSLRNALDVAAEHDRRIIVEAGVD
                     AREIEVSVLGNEDPEVSVPGEVLVTANEFYDYEAKYTEGGSKIVAPADIPDEIASEMR
                     RVARIAYESVDAAGMSRVDFFLERGTDRLLLNEINTIPGFTPTSVYAYLWDASGLPYE
                     QLLDRLIQLALERHG"
     gene            complement(21431..21994)
                     /locus_tag="VFJ72_15095"
     CDS             complement(21431..21994)
                     /locus_tag="VFJ72_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7480830.1"
                     /translation="MNPMSRDDAWNLMCEWTESDSLRKHMLAVEAAMRAYARHFGEDE
                     EKWAVTGLLHDMDYEKHPTPAEHPMVGVRELERLGYPDDVLHAIKGHADYLDVPRDTL
                     MSKTLYAVDELSGFIVACALMRPEGFEGMKAKSVRKKMKQKSFAAAVSREDITRGAEE
                     LGVDLNEHIEFVAAALRERAGSLGFAA"
     gene            complement(22305..22769)
                     /locus_tag="VFJ72_15100"
     CDS             complement(22305..22769)
                     /locus_tag="VFJ72_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480831.1"
                     /translation="MAALLLAAGCSTGGDTDEGSGSTGGSGKQTTAFEEPTISEESSQ
                     DETTTNATVQETTLGAGKEEPDVVLRLEGDPKTRFSGRCTDGKQETVLKGKVPKRYAY
                     DLAGDELQCSIQKRDAGNGSLKVILTAGNTTRSVQQTNTRGGEIKISYSSSG"
     gene            complement(22844..23533)
                     /locus_tag="VFJ72_15105"
     CDS             complement(22844..23533)
                     /locus_tag="VFJ72_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET7480832.1"
                     /translation="MSARALAALEAEITTCRRCPRLVAWREEVARVKRAAYAVEDYWA
                     RPIPGFGDPAARVVLLGLAPAAHGANRTGRVFTGDRSGDFLFAALHRTGFANQPVSRG
                     RDDGMRLTGAWISAAVRCAPPQNKPTPAERDTCLPYSARELELIGAKVIVCLGAFAWD
                     AALRLYKVRPKPKFGHGAEYEADGMTLLGCYHPSQQNTFTGVLTPPMLDSVLQRARQL
                     SARQSSLIVSD"
     gene            complement(23530..>23811)
                     /locus_tag="VFJ72_15110"
     CDS             complement(23530..>23811)
                     /locus_tag="VFJ72_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480833.1"
                     /translation="VEIIGAGPRANARRDAVLEAFAQMLDEQNAIAHERYGAPRFASP
                     DDAFALVGAVVELASRQLRHGRPANIQELEPVIARFVRGLLDQGTPAPS"
BASE COUNT         4350 a         7081 c         7762 g         4618 t
ORIGIN      
        1 ggcaaaactc gcccagcagg aggctactca gaagaaagcc gatcaacgcg ccgctgccga
       61 gcagcgggcg gaggagagac aggccgccgc ccagcaggcc cgtgacgccg ccgaacaggc
      121 cgcgatagaa gaggccgctg ctaagcaagc cgccgaacag gccgccgccc agcaagctgc
      181 cgccgaacag gccgccgccc agcaggctgc tgccgaacaa gctgcggctg agaaactggc
      241 tgcgcagcag gccgccaagg aagctgctca acaggccgcg gcggaccagc aaaccacgca
      301 gcctactaca gactccggtg ggtccacagg tggatctacc ggcgggtcca cgcagaccgc
      361 gccggctccg accggtggtg gcggtgggtc cgcttcggga tctgcggttg tcgccgaggc
      421 tcagacctgg ctcggtgtgc cttacgtata cggcggggcg tcgcgttccg gcgtcgattg
      481 ctcgggcctg accatgctgg tcttccagaa gttcggtatc tcgctgccgc actcggccgc
      541 agcccagtac gggtacggct ctaccggctc cggcaccgct ggcgacctgg tcttctggga
      601 tgagggcggc ggcatcagcc acgtcggcat cgctaccggc agtggaacga ctatccacgc
      661 tccgtacccc ggaacagtgg tgcgcgagga gggtatctgg tccaacggat acgtgggagc
      721 taagacgctg ctctagcgct cgctgtcaga caaccacctt gcaatggaga gggaggcccc
      781 gcggggtctc cctcttatct cgtggagcga tgctagaatc aactatcgca ggccggataa
      841 ttggagcttg cgataacgtg gaaggagtac ctcgctcatg agtctttcgc tactgcaggc
      901 gttcggtttc gggttcgcgg cgatagtatt ggtcttcggc gccatagtct tcgtctctac
      961 cattctcagc gccgtcaaga tcgtcaacga gtacgagcgg ggtgtgatct tccgcctcgg
     1021 tcgcgtactc ggagcgaaag gtcccggcct attccttttg gcgcctatct tcgaccgtat
     1081 ggtcaaggtg gatctcagga ccgtcacgat ggacgtgccg ccgcaggacg ttattacccg
     1141 cgacaacgtc cccgcaaggg taaacgccgt tgtgtacttc cgggtcgttg acccgaacaa
     1201 gagcgtcata gaggtagaga actacgtgct ggctaccagc cagatctcac agaccaccct
     1261 ccgaagcgtc ctgggacaga aagatcttga cgacctgctc accaaccgcg aggcaataaa
     1321 cgacgagttg cagaagatca tagacgagca gaccgatccc tggggaataa aggtcagcgt
     1381 ggtcgaggtc aaggacgtgg agataccgca gcagatgcag cgggcgatgg cccggcaggc
     1441 ggagagtgag cgcgagcggc gcgccaagat catcgccgcc gagggcgagt accaggcgtc
     1501 cgagaggctc cgtcaggccg cggaccggct ggagagcccg accgctctgc aactgaggct
     1561 cttccagact ctgggcgagg tggcgaacca gcagaactcg accatcatcc ttcccgtacc
     1621 aatggacctc ttccgaccct acctgaacgc gcatgagagc ccggccaacg gccgaaccgc
     1681 gtcacgcgcc gaacggcaaa cttctgatgg ggatcgtcag cctgaacgcc tctacgagaa
     1741 aaccgtcgaa gagacacgcg ccggcatgcc tcaagctcca gccgaagagc cgccggaccg
     1801 cgagtttcct ggcgcatccg ggatttagac gcttcaatca agtcctatcg aggcgcctcg
     1861 tctcgcgagg ttagcttaca gtatcgctgg gtaacatagc cacgaagtag caaagcttat
     1921 ctttccagaa aacttccgca agagtagtgg gagaaaattt cgggggagtg aaagatcaga
     1981 tgggtcacga gacaacggag acaaagaggg gagaggagat cgggttctct gaaccaatcg
     2041 cgctcaggat tcccagccgt tccgagtatg tgctgctagc tcggctggtg gtatcccagg
     2101 tcgggcagtt ggccagtttc gagcccgaag acatctacga cctgaagctc gccgttaccg
     2161 aggcggcgac aaacgtcata cgtcatgcgg ccgtagatca cttcgacatc gaataccggg
     2221 tctcccggca tgcggtcgag atcaccgtga ccgacgccgg tggcggcttc gacgccgacg
     2281 gtttgaccca tgctccggac gagcgaggcg gctttggtct ggccgtgatc cagaacctcg
     2341 tcgatgaggt cgtactcgat acggccgctg gcggcggaac tcgcctcaag atgatccggc
     2401 gcgcctccgc gaccgtatag ttttttcgag ccagttaggc gtagaacagc ggttttacgg
     2461 acgtccttgg cgttgatttg cgcttccgcc gtccactgta tacagttagc cgcaaatttt
     2521 gtgtaaccga ccgccagagg acgtcgtgct acaacgcgta aaccccggta cgaaatctct
     2581 cgccgactac cgcagtatca tccgccggga gctctacgac gagattcggg atctctcggc
     2641 gcggttggat ggcgctcgtg tcctgcatgt aaacgccacg agtttcgggg gcggcgtcgc
     2701 ggagatcctc tacacgctcg tgccgctgat gcgtgatgtg ggacttcagg ccgactgggg
     2761 cgtcatgttc ggcgcggagc cgttctttaa cgtcaccaag ggtttccaca acgccctgca
     2821 gggcgccgac tacgacctta cagtggaggc ccgcgctatc tatgaagagt acaatcgcca
     2881 gtcggcggcg gcgctcgtag aggccgggga agagtgggac ctcatgttca tccacgatcc
     2941 gcagcccgcg ctactcaagc acttctccgg cgggctctct gagaagacaa agtggatctg
     3001 gcgctgtcat atagatacct ccacgccgaa caagcaagtt ctcaactatc tggcgcccta
     3061 catcgaggac tacgacgcgc agatctacac gatgcaggag tacactccgc cgggcgtcga
     3121 actgccgaac ctgacgctca taccaccggc gatagaccca ctctcaccca agaacatggc
     3181 actctctcca gacgacgccc gctacatcgc gaaccagttc ggggtggata ccagccggcc
     3241 gttcatactc caggtctccc gcttcgaccc gtggaaggac cctctgggtg tcatagacgt
     3301 ataccgggcc gtcaaggaag aggtgcccga gacgcaactc gtccttatcg ggtccatggc
     3361 tcacgacgac ccggagggat gggactactg gtacaagacc gtcaactacg cagccgggga
     3421 tcaggacatc ttcctgttct cgaacctaac gaacgtcggc gcgatagagg ttaacgcctt
     3481 ccagtcgctc gcggacgtgg tgatccagaa gtccatccgc gaggggttcg gtctggtcgt
     3541 ctccgaggcc ctctggaaag gacgtccgat ggtcgccggg cgggtcggcg gcatcccgat
     3601 gcaggtaacg gccggcggcg gcatcctggt ggataccgtc cccgaggcgg ccgccgcctg
     3661 cgccaagctc ctcaaagact cggagttcgc cgctgagatg ggccgccgcg gcaaagaaca
     3721 cgtccgcgaa cactacctaa ctccgcgcct tctgcgcgac gacctggcgc ttttcgccaa
     3781 actgctcggc gtataacgtt gcgcagaagc tgaggactct ccgcagcccg acaagatcgg
     3841 ctcttatgct tccgggaagc ttgctaacag cgcggtagaa cgccctcttc tgtgaggaac
     3901 ctttgtaggc tcatcccgct ctctttgagc cgctcggcat ttcgcacacc gatatagcgg
     3961 tagtgccagg gctcccactg gtaaccggtc tcggcctcca ggccctcggg ataagacaac
     4021 acgtagccgt actctccagc gttcttcttc agccatgtgc ttccgctcgt gcggccgaag
     4081 ggctgccaga tcttgtaccc cgccgcggag ttggtgaagt ccacggctgt gccgagttgg
     4141 tgctgactgt ggccgggaag agcgctcatg ccgcccgcgt tcgggtcgcc gtagaagttg
     4201 gcccacttgt catgcgaggc ttgttggtcc gtatatgaac ggtaggaaga cgcgattacc
     4261 aggtcctcgc catcggccgc agccgcagcc atcagaagct gcagttgccc ggcggcctct
     4321 cgccggagta actggtctcc gtccacgacc ggcacgccga acgcgctcag agagacgagg
     4381 tcttgtggca catatccgta ggggagggga tgcttatgat ccaccagtac ccgcagatcg
     4441 tcacagctct cggccggcgc gggtagaggt ggtcccttgg ctatctgctt ctccggagcc
     4501 ttcgcctttt tgtcctgttt cgctttcggg ggtgcgggag cgtcttttac cgctgcggcc
     4561 tctccagtcg agaataccag cggggaaggg ttgtcgcgca gtaacgcata accggcggtt
     4621 gccagaaggg ccagcatgac aatggcgatc gcgagccgca tccggcgccg gtgcatgcgg
     4681 gcggacgtaa acacggtttt ggacagacgt ttccccaaag gtttcacgaa gacaaagcta
     4741 acgcactatg tcggtactgt ccagacaaag taagagacaa cgggcatcaa ttggacttgc
     4801 tggaggtact catagtgcga gatatctggc gaacgatgcc ggagaccgca tcggtgagag
     4861 cctgccggtt tgagagcagc ggtaacctga agccaatctt cgccagaagg tccaggatca
     4921 ggcagacttc caccgaacgg ctggtcgcac cgagtagagg ggtagtgaac acccaatcta
     4981 tgtaacggac gaagtgaaag ctactgccaa ctctccaggg tccgcctccg tcgcgccatc
     5041 ctaagagaaa gccgcgatgc cggtgatggc cgatgtgatg ataaagagat gacggtcccc
     5101 ttccggagcg cgggacatcg agaagaacac cgtagcctct atgaagccag ggtccctgta
     5161 gtcagccaga gagtctgcat atgttactct tcccgcgccg gtatacgaaa gcgcgggaac
     5221 actctccaac ttacataccg ttacataccg ctgcggcggc cctgggcacg gtccctgatg
     5281 tcgtcgacct cgatctcgcc gtccaggaac ttgcctatga agtaaccgat aatcgcgaag
     5341 agcaccacga gagccgcaac gccgattccg tagacggcga tcaggaatat cacgagcccc
     5401 ccgatcaaag ctccccactg tttgttggtc caggtagcca ttacaacctc ccctttcgtt
     5461 taaaccacgg ttccttgtac tgtgacttcc acgtccttta cgggcacgct ttggccctcc
     5521 agcacctgcc ggatgttctc tctggtgcgg gtcgcaagct ccgtgtagtt gctggtgcgt
     5581 ggagctttga tcttgcacga aaccaggtaa gttcggtctt tagaagtcaa tgacactgat
     5641 ggagagaccg ctccgacttc ccaggcggcg ccctcggcga gcgtctttac ggccctggcg
     5701 gtgatagtcg tctctttacc cggcgtgtcg tctatcagcg tgctccgggc gacgcgccgt
     5761 ccgaaggtta gctcccgcag cagaagtaac aatgcgacga gcgctaccag caacccaatt
     5821 atgccgatta tgatcccggc gtttccgctg aggttcgacg gagagagatt gcccagagtg
     5881 ctaaccgcgg cgctgtagtt cgtgtactgg tcaatgagac gagccgaaag cgcgccaaac
     5941 gccaccagga gcaacagaac gggtattgct accaggagca gcgctatgat cagcatgatg
     6001 aagcggttaa atgcgttcat tgcaccctcg tcttcgtctc tcgcggatcg gactcgacca
     6061 tccggatctt gagccggttc acgggtatgc cgctgctggc gagatgctgc tggacccgat
     6121 tttgtagatc actcttgata gtgtttacct catcgccacg ccgcacgttg gccgcaagat
     6181 ctatctgcgc gccaggacgc cgccgggcct tgacccggac cgaggaatcc agcacgttcg
     6241 gcgtctcctc cgtagcgtgc tcgacctcgc cctctacggt accgcgcgtt atatatgtgc
     6301 ctttctgcat ccgtacccgg cgtggcgtgc gaggtttcaa ctcggcgata aaaaggaaca
     6361 gacccagcag ggctatcaat gccagggtta tggtcatcaa gaccgggagg ttcccattct
     6421 cgacgccgct cacgaagtcg ctcacaccct ggccgaacga ggacaccggg agatcggaga
     6481 gttggtagcc aaagagattg aacccatagg caaccatgta gaccccgagc aggaacagtc
     6541 cgcaaaagag cagcaccatt actatcctgt taaagattct catcggcacc catttcaagg
     6601 tcttccacta cgatcctggt cgtccggctt ccgacctgtg gagctactcg cgccatgatg
     6661 ttgctggcta tctccgggat agggctgggt agcgggtagc gcgccactat gtgtatctct
     6721 acctcatccg gcttcaccac aacgcccgtg actttctccc ggccctcata ggtggctgct
     6781 tctgcgtagc gtccgactcc catggagtgg acgccttccg tggcaagggc cgcctccgag
     6841 gcgacccttg caagctgtag ttcgtcagcc aaagcgcaac cagatctctc tactggaccc
     6901 gctgctgttg ttcagcttct tgctgacgct gctgttcgcg ctgctgctgt tcgagctcct
     6961 tctggcgctg ctgctccgcc tcctgctccg ggaagaatat gttgctgacc gtgatgttga
     7021 cctcggttac ccgcaggccg accaggctct ccacccggtt gacgacgttg cggcgcacgg
     7081 cctcggcgag ctgcggtatc gatttgccgt aagcggccgt gagggtcagg tccacagcag
     7141 cctcttcctg gccgacctcg actgacacgc cctgcgtctg gctgccactg ccgctgccgc
     7201 cggtgatgct gcccagaagg ccgctaacgg cctgggaagt gccgccgccc atccggatac
     7261 cctcgacctc ctgcgccgca ataccggcga tcttcgagac gacggtgtcc tggatggtgg
     7321 tgttgccgcg atcggtctga agcgggctgc tgctggagct gctcgcgcta ccagaaccgc
     7381 taccgccagt gcttccggtc ctctgctcag tcattctctg ttctccttcc cctcattagc
     7441 ttgggtcttt cgtttacgat tgtcggataa cctgtaccgg ctacgccgcc cggcgggtga
     7501 tcaacctgta cactgccagc aggatgatcg ctcccagagt cgccatcaaa acagaaccta
     7561 gagataagtt ggctccgcct ccgccaaaca acggttttag gagaaagccg cctacgaagg
     7621 acccggctat gccgatcaat atagtaacga tgatgccgcc tgggtccggg cccggcatca
     7681 cccatttcgc cagtgctccg gcgatcaggc caattattac taaaactatg acgcccccga
     7741 tgcttatgcg ctctcacctc cccctaccgt gcgagtaaat ggcctgccgg cgacatccag
     7801 tccgcaatat agcagatgcc acgctttgac tacttcgtac ctgcgcttca tacccccgct
     7861 taacaccgca taaacgccta tcctggagga cggacgggaa ctaaatgtgc tctggagtca
     7921 cattgaggtc gcacaccatt ccgtatcctc ttgagcggaa cacaagagag aagcgggtca
     7981 aaagatgcta atcgaggaaa ggcttgttct ttctccagat cagatagatc accgagagta
     8041 tccagaggcc gatgatgatc agatacgcag ggaagagttg ccggtagtac tcgggagtgt
     8101 cgctgctctg aataactggg attgtctcct tgtgaggcag tctggcttgc tttagtccgg
     8161 cctgggtggt gtcgtggaaa ataaccgtgc ccaccgtggc tagtttaccc ctgaaaacat
     8221 gcggagggtt ctggaagtcc ggtggagtct gcgccttctg gatcaggagg ttgtcaccgg
     8281 tgttcttcag gcggaagtag aagtaattgg cgctcgtgta acgcgacact acccccacct
     8341 cagattccgg tgtgccgagg acgcgggtct ccgagccaaa ctcagggatg ccccggacct
     8401 cgacgtagtc gccctgctct atacctgcgg gcaatttacc gcgattgatc tgctcggcgg
     8461 tgatgacttt cggatcacct accagaccga acgagaggtt agccaccgcg aggtatccgc
     8521 agaaagtgaa gaccatgacg atgaacagcc ggaccagcaa ccaggggttg cgacggatcc
     8581 aagccccaag ccccggcggc tcgtagtggt caccgtatcc gccggaggcc ggcgtctgtc
     8641 tatcactgcg ggtatccaca ccatgcagtt tacccgagga ataacctgca ggtggggccg
     8701 cgttcacagc gccgcagggt cgccgggcgg cagaatgtga acgagctatc cgccgttggc
     8761 gggggtgggt ggtgtagcag gttccaacac tgtatgctgt tacccggcga aaatcaggta
     8821 agagggtgga tacgggatac cctccgtgga ggtaggtttt ggaacgaggg cgggaagagc
     8881 cgaagtccgg ctccgagtac accgttatag gggtgataag tgacggggcc gagctcaacc
     8941 aggccgtgac ggaggtccgg gagctcggtg tgggccggga tgacctgact gtgatattga
     9001 agcgtcagga cccatccgag cccgagccgt tccccgaggg gacgcgctac atcgtgatcc
     9061 ccaacgaccg gcgcggtctg gaggtcccaa taggattcgc gatagccttc gtcgtgttcg
     9121 ggatcttctt cgcgctctcg accccggcta tcggcatccc gacgctgatg gtgttcgtct
     9181 cgctggcgtc catcctggtc gcgggctcgc ttacgcgggt gggagtctcg ccgatactca
     9241 cggagatgga ggcgccgagg gaagaggccg gtacctggaa cgacgagttc gagatgggga
     9301 aagtcctcat cttcgccacc acgcgggagc gccgtctcat ccgcccgatc cgggaggcga
     9361 tgcagcgcgg cggcgccatg tactatatcg tcaaccgccg gctggagccg agggccgtgc
     9421 atcaagccat cctgaaacgc gccgggtcct acgccgattc gataaccggc ggaccgaaaa
     9481 gccgtgtcgg ggagacccgc gataccgcct cagaaggcac tagataggag tttagagcgt
     9541 gggcacacca accatttcga cactccagtt tctcgcgcag ctagacctct ccaatacgtt
     9601 cgggttcctg ggcggggcgc gggttattac gggcctctcg atgctcgtcc acatgttctt
     9661 cgcgcagata ttcgtaggct ttgccctggc cgcaccggtg ttgcaggcgt ggggcgcccg
     9721 gacgggcagc ccgcgcatgg accggctcgc gcactcgatg gcccgcttca atgtcctgac
     9781 cttcagcacc ggcgcgacgt tcgcggtcct ctttatagtc cttttggtag gtttctatcc
     9841 cagggtgacg gcgtcgctct tcactcactt cttctacttc ctcgctatag ccatgctctc
     9901 gatggtgctc tcgctgtggg ggatgtacag ctactattac aagtgggacc ggtacagcgt
     9961 gctcaagaag aagcgccaca tagcgctcgg tttcacgatg gggttcttta tctggatctg
    10021 gatgctcata atgacgggca tcgacaccta tatgacgacc ggcgggggac cgaacagcac
    10081 gccgctcaac ggcaacagca tcgagagcct gggcgtggcg ttccagggca tcttcaaccc
    10141 gatgttcgtc gagatgctac tgcaccggac gttcgccaac ctgtcgtggc cggccttcgc
    10201 gctcgccgcc tgggccgctt tcatgtacat acgatccaag acgcaagaag accgggcgtt
    10261 ctatgactgg agcacctccg tcggactgac gtggggtacc gggttcctga tcgtgcagcc
    10321 gctcgtcggc ttcgcgatcg tatacgcgat gaagcttgcg gtgccgcaga gtccggccga
    10381 ggagactgcg cggggcggcg cctacgagcg gctcacctcc ggcgacacct cgaacatcct
    10441 gtacctaaac ctgctcatgg tcgtgggact cttcgtgctc tcgaacatag cgatgtatat
    10501 gggggcgggc cggcacccgg accggggcgg gcgcatcccg atccgcttct tcgggctcgt
    10561 ggcggcggtg gcgggactgt actcgatctc gccgctggcg agctttccgt acgagtcgcc
    10621 gttcttctac ctgcgctata tcgccatcct gataatgctg ctcgcgacat tcggggcgtt
    10681 catcacttac ctgcggggcc ggatgcgttt ccgatacgga tctccggggg cggcctatcg
    10741 tgtcacgctg ctcgctctgg gggtcctcgc ggcggtcgtg gcgctcgata tgggactaat
    10801 gaaatccaat tcgagggtgc cgtacacggt atataatcag cccgattaca ccgtcgagag
    10861 cggtccgccg ccgagcggat tcggcaggta gggtcggcgc tcggtgagag tctagaaggg
    10921 gccagacgtt gacggagttg cagaaagatc ccatcactcg gagtgacttc ctgggctttg
    10981 gtttcatagg agccatcatg ggggcgatac tgaccatccc gccggtggcc ttcgtgctgg
    11041 gtccggtcat aaagacggat gtgctcggca agtccgatat aagcaagggc tggcaggagg
    11101 tcgggaaggt tagcgagatc aaggcgggtg agccgaagga cttcacgata gagtttccgc
    11161 tcacgcagtc ttacggcgac aagagcatcc aggagaaagc tccgagcagc ccgaggtcta
    11221 acaagcagtt cacgctccgc aacgccgtct gggcgtcgtg gaagacgaaa tacaagccga
    11281 acggctcgca gggcaaggtc agccggcctt ccttcctcga ccagaagacc gagggatttt
    11341 cgcagtcgga gataaaggag atcgagaaga acctcaacgt cctctccaat tcgtgcgcgc
    11401 acctgggatg cccggtgcgc tgggtgatca ccgagggcga gggcgagttc ctgtgtcctt
    11461 gccacggcgg cctgtacgac attaacgggg actaccaggg aggtccgccg ccgcgaggta
    11521 tgtatcacta caagacttgg gaagtccgcg agaacggcaa gctctacgtc aagcacggct
    11581 acatcatcca ggaccgtccg gacgctacac agccctacgt ggtgtaggga atattaggga
    11641 gcgggcatgc agagaggaga accgaggaca tgatcggcag gctgaggtcc cagacttcat
    11701 cggcgagcaa ggtcatagtg gactggatgg agcaccgggc cgggatagtg acggccatgg
    11761 agcacttcct ctacgagccg gtcccgaagc ggggagcgtg gctctacact ctggggagcg
    11821 cggtgctctt cctcatatcg ctccagttcc tgacgggcat cctgctgctc ttctactacg
    11881 tgccgacgac ggaccacgcc tggaactcgg tctactacat aatgaacgag gcgtacttcg
    11941 ggacgctcat ccgcggcatt cactactggt cggcgaactt cctgatggcg atgatcgggc
    12001 tgcacatggc ccaggtgttc ttctccggcg gctacaaggc gccgagggag cttaactgga
    12061 tcgtgggcgt cgtgctcttg ttgctggtca tcgggctggc gttcaccgga tatttgctcc
    12121 ggtgggacca ggacgggttc tgggcttctg tggtgggtat caagatcggc tcctactctc
    12181 cgtttgtcgg ggcgtatata acgcacttcc tgctcggcgg cgatgcggtc ggaccggcta
    12241 cgctgtcgcg cttcttcgcg atccacgtat ggctcttgcc tgcggcgata gcgccgttta
    12301 tcgggattca cctttatctg ctgcggcgcc acggcgtctt cggcagcgag ttcgagtaca
    12361 gcgagcggat cgccaagatg cacgagcatc agcgcatgga ggagtacgct cgttacgagt
    12421 tgagcgacga tgagcacgag gaagaggacg gctacgaggg gaacggccgc ggccggtacg
    12481 acggaggacg ttagcgtcga gtaagagtat ttaccgaggg cggatgcggg atgctgctat
    12541 gatccagaca caggttttcg gaagagaggt gtaggtatgt acgctccggc gacaggcaaa
    12601 gccaaaccgg tcgaggaaga agtaatcacc gaggagaacg tctttgaccg tcccgacgag
    12661 acgatagggt tcttccccgg acaggtgctg aaggacgctt acgtctcctt cttcctgcta
    12721 ctcgcctgct gcgccctctc ttacatcgct ccggcgcctc tcacggcgcc cgccaacacg
    12781 gccacgatca cgtacgtgcc gaggcccgag tggttcttct tcttctacga gcagatgctg
    12841 atgttcttcc ccggttacgc gctggtgtcg ttcggggcgg tcgtggtgcc gacgatcttc
    12901 attgtgacgc tctttgcgct gccgtggctg gaccgaggtc cgaacttcag ctccctgcgg
    12961 cggcccttca cggcggtggt ggggatcctg atcatagtgg tcgtgctgtt gaatatgctg
    13021 ctggcggtga gccgcatcat gaacttcccg tcgggcgggt agaaggagca aaggatgccc
    13081 ataggagatc tccaggtccc cgtaatcggc aagaacgtgg tgatcgcgtc tctggtgcag
    13141 acgcacatcc tgttcgccgc gttcatcatc ggggcggtcc tgatcgccgc gacgagcgag
    13201 tacctggggc tggtgaccaa gaacccgcgc tacgaacggt tcgcccggaa cctggcgcgc
    13261 ttcgtggttc tcctcttcgc ttccggcgct gctctggcga tcacattcgt gctggcgctg
    13321 gtgacgctgt tcccggtttt cttttcttac ctgcagaacg tctttttctg ggtgttcctg
    13381 gtcgaggcgt tcatgttcct cggacagatc atcatcgtgt acgcctggta caacgtctgg
    13441 gacaagctgg cctatcgcaa gagcctgcac gtggtcttcg ggttcgtggc tggcttcatg
    13501 gggctgatgg cgatgaccat gatcgatggg gtcgcttcct ttatgctcac gccggcggag
    13561 accgaagttg gcaacgtcgc ccggacgttc ctgaaccaga ccaacgttcc actgaacatg
    13621 caccgcttcg taggcaactt ctcctacgcg gggttcctga tcgccggttg gggcgcctgg
    13681 cgctacctgc gctccacgcg ggaggacgat agagagtact acgactggat gggccacttc
    13741 ggcctcatct ggggcttcgg gttcctgatc atccagccgg tcatcgggta cgggtacttg
    13801 aagggcatcc gcgagagttc tccggacgcc ttcaacactc tcatgctggg cgacaaggcc
    13861 tggctcttta acttcctggc gctctggctc gcgataatga gcatcgcctc cacggcgtac
    13921 ttcgtgcata agctcaggtt cgcggtgaag ccgatggaga ccctgcggaa gctatccgtg
    13981 ggcgctctgg gcttcacggc gctcttcacg gtgctcaacg tcataccggc aaacacttcg
    14041 ttcatcccgc agatagggct catattcctc ggcggtgaga agacgaagat accgctgggg
    14101 agcatgtacc cgtacaaata catcggtctg atcggcatga tgctcgttgg aattttcgtg
    14161 gtggcgctct acctgcgggc tgcggcgtcg ggcttccact ggggacgggc gagccggtgg
    14221 agccagttcg cgctcctggc ctgctcggtc acggtggtcc tgacgatggt gacgatggga
    14281 tatacccgcg agacggcgcg ccgggtagat tacacgcccg ccggtaacgg tggcgggtac
    14341 ctgatctaca agtgcatcac cctggatcag aaacccactc cgagcacctg tccgacaaag
    14401 ggactcgggg gaggagggga gtgatggacc tggcgctgct tcttcaggac ggggccacat
    14461 ccggcatcgg gatcttccgg ttcttcgcgg gcttcatcct cgtgctgtgc ttctacctcg
    14521 gcgccctggg ctttacgtac tggatcagca aggacgagta ggttagaagc gtcaagctgt
    14581 cagctatctg agaagggtgg cgccccgcac cggggtcgtc gcccttcttc tcctggaagg
    14641 ttgttccggc tgcgccacag ggcgcgaagc tgaggaccag cggctagttg atctggtcgg
    14701 taggacggag gaggacttcg tcgacgctca cggaacgggg ctggcttacg gcgtagagga
    14761 ccgcttccgc tacgtccccg gcttccagcg gggcgtactc tttctgagcg tagtattccg
    14821 cggcgctgga gtaggagttg ttggggaact ccgtgcgtac caggccgggt tcgaccgaga
    14881 taacgcggat gttctttcct tctacctcca tgcgtagccc cccggttatg gcggagaggg
    14941 cgtgcttggt ggcggcgtag acggtgccgt aggcgttcgg gacccggcga ccggcgacgc
    15001 tggagatgaa gactacgtcc ccggagccgt gggggatcat atgacggatg gcagcgcggg
    15061 tgcagcagag agcgccgagc acgttgacgt cgaacatcct gcgccaatct tccgggtcgg
    15121 cgtcggcgat ggtggagtag agtccgatgc cggcgttgtt gacgagaagg tcgagagcac
    15181 cgaagcgctc tacggcccgc gtcacgaggt cctcgcaggc ggcgggatcg gtgatgtccg
    15241 ttggtacggc gagagctctc tgcccgagag cggtggcaag ggcctcgatg cggtccgccc
    15301 ggcgcgcggc gagtacgaca ttgtagccgt tctgcgcgag caggcgggcc gtggcctcac
    15361 cgatacccga tgaagctccg gtgatgatgg ctgtgcggct ggccgggcta tgagcgctca
    15421 cgaagacatc atagccggtg agagagccgt gggccgggtc gtgagataga attcccgtgg
    15481 tagatctctt aaggagtgaa agtttgtcag acataccgct cttccccttg aacgtggtgt
    15541 tgatgccggg ggctccgctg ccgctgcaca tcttcgaaga tcgctacaag cagatggtgg
    15601 acgagtgcct ggagtccgag agcgagttcg ggatggtgct ggcggatgag gccggcacgc
    15661 gggaggtcgg gtgtacggcc aggatcgtgg agctcgtgga gcgctacgag gacgggcgga
    15721 tggtgatcct ggtcgaaggg tcgcggcgct tcaagctcaa cagcgtcatg accggaaagc
    15781 cgtactacgt aggagaggtc gagtatatcg aggaggaacc ggaggaagac gtaacggagc
    15841 tggccgagga gtgcaaggcg ctcctcgagc gcgttgtcga ggcggctacc gaaggatcgg
    15901 tagccatcga gatagagcct ccctacagga acctctcctt tgcgatagcc gggcgcatcg
    15961 agttcgacgt ggagacgcgg cagcagatcc tggagctgat gaccgagcga gcgcgtctcg
    16021 agaaggtgaa ggagctactc tcctcggcgg ccgagcgcct ggagcgcgac cggcaggccc
    16081 gcgagaaggc tcagaccaac gggcacctgc gccccaacta ccggccagga gagaagcccg
    16141 aagagagtcc ttcctagtcc tctttatcta cctcctgggg tatgaggtct cttatgcggc
    16201 ggaggttggc ctggtccacc ttgtagatca gcggctccga tgccagagcg tcctcgccgg
    16261 cggcggcttt tatgcgcccg gtgaggttct cctcgacgag ccaggccatc gagggtagca
    16321 ccccggcgaa gatccgcagc cgtatccgac cttcgttctc ctcctggccc acgagacgcg
    16381 ggggcgtcag gtagaggttc gaagcctcgt cgagcgaacc caggactcgc tcgaccgcct
    16441 cggggtcgga gagctgcacc tcgacggtga agcgttgctg accggagacg aagttcgtca
    16501 cgctctgcat cgtgccgttg gggatgtagc tgacctcgcc ggagagcgac cgtatcttgg
    16561 tcatccgaag tcccaactcc tccacgatac cggagatgtc ttgcggtttg atctggatga
    16621 agtctccgac ggagtactgg ccctcgaaga tgatcgagaa tccggcgatt acgtcgcgca
    16681 ggaagctctg cgccccgaag cccaacactg cggcgaggat cgtcgcgccc gccgtcgcgg
    16741 gcagcgggtt gcggacgaag atggagagca cgaacagtat gatcaccacg aagaccacgt
    16801 agcgcagggc attgcggatg agcgttatgg ccgtatcctg gcgcttgatg cgggcgaccg
    16861 cctccgcatc caggacgtcg cggtggctcc gtcgccagcg caggacgcgc ggcacgccgc
    16921 gcgtcaggat cctgtaggcg aaaatgccga gcagtatgac taccaccgaa gcgatgatgt
    16981 tgcttatgaa ctccgcgccg gtcaggtact tgaaagaggc tgcggcgaag tcgttgatcc
    17041 ccttgaagac gtcgaaggtg cccttgctgg cgcctttcgc ggcgtctccg gccttgtccg
    17101 cggcggctct gatgctgctc gtggtctctt cggcgttgtt ttgtaggaga gctataagca
    17161 tcgcggctgc acattaaagt atcctgaccc accgacttca aggtggtacg ggatcgaacc
    17221 tccagaccgg agcggtggga ggggaccgga ggcaggcaac tccggtcccc ggaaggaggg
    17281 ggcaaatggt tcgcgacagt cctggctaac cgacgatccg gcgggcgagc acggcgccgc
    17341 tgatgagcgc ggcgttgatc gccagtagcg atgagaccgg gatcccgcct gtatccggga
    17401 gtacgctgac cgcagcagcg gcgtccccgg tgtcctgatg ggatggggta gagtcgcctg
    17461 tcccggagct gtccgcgccg gaagtgtctg tatcagattg atcgccggga tgagtagcgg
    17521 agccattatc cacgcgggta acgtcgagga gatctggccc accctcgacg gctccgttct
    17581 ggtagccgga tacgcgggcg ccatggaccg ttacccgctg gcccacatag tcgtcgaggg
    17641 tgaccgtttc gctccgcagg gcgaacagga gattgccgct ctcgcctagt aggacgtggg
    17701 tgccgtactg gtaggacgtt atcccggctt cccggacggt tccctcgacc gtcacggcgt
    17761 cacccgtctc ctcgatatcc gcaagtccac cgtactgatc ggcaacgccc gtcggtgcgg
    17821 agcctggatc ctgagccagt gccggaacgg cgagcgtgaa tatcaggacc aagatcatga
    17881 tagatagctt tgtcatgagc atctccattc tccggtgatc ttcataatgc ttaacaatta
    17941 tcggtggccg gaggagaggg ggcgtcagcc gaaaggatga cccggctcgc cagatggatg
    18001 attgtccgcg gggcggcttt ctcgcggggt ctcggatgca ctagactgtg gtcatgttcc
    18061 gttccaggat agcaagctcc ggtgcgcgat ggtggtggac gctcgccgcc ggggcgggaa
    18121 tagcgttcct gaccttcgtg gtgatgttcg tcatgatcga cgtgtacctg accattgcca
    18181 cgatggtttc gcggggagat gtggatcaga gccaggtcca gaccttcgcg gatttcgcgt
    18241 ccgtgtgggc gttgccggtg ctgtttctgg tcctgactgc gggagcgacg gcgtggatgg
    18301 cgcgccggtt cggcgttacg agcctgtggt tcggggtctt ggtcggcgcg atctccgcta
    18361 tcggcttcca gctcatcggg ctgctcttca gccctcccga gacccgcgag ttgatcgtct
    18421 accctctctg cggggtggcg ggaggtctgc ttggcatctt cctcggacgg atctccatcg
    18481 ccgggcgtga ggcactctac cgcgccagcc gggacgtggg agcagcgtca agcgcggacg
    18541 aggtcgcttc agccatcggc agacacctgg cgggctcgaa cgtggagcag gtctcgctgt
    18601 ggagcgtagc ctccagggaa aaggacgagg acttgaggtt ggatctcacc ggctcatgga
    18661 gcgcgcggac ggaggcgcta tggccctccg gcgcgagcct cgattcagaa ctgataccgg
    18721 atctcgtcgg gatgcggcgg cagtcgccga gggtcgtccg cgggcgagag gtgccggagt
    18781 cagaacgagc gaactgggag cggcgtggcg tgcgcgctgt acttctggtc cccctcagtg
    18841 cttccggcga ggggccggac gggttgctgg ccgttgcttc gcggggcccg aggttctcgg
    18901 ccggtgcggt gcgcgcgtac ctgacggtcg gggcgcaggc ggcgctcgcg ctcgagaacc
    18961 tgcggctggt acgtggggcg cgccactccg ggatgatgga cgagcggcgg aggctggcgc
    19021 acgagatcca cgacaccctc attcaggggt tcgccagcat cgtgatgaac ctggaggccg
    19081 ccgaagggaa gtccggggac gacccggcgc gggcgtacag acacctgcgc gaggcccggc
    19141 gcacggcgcg cgagagtatg gacgaggccc gccggatggt ttgggccctc agacccgaag
    19201 ctcttgaagg ggcctcgctc tccgaagcct tctcgcgtct ggcggagcgc tggtcggagt
    19261 caaacggcgt cgaggccgtg gtcacggtta ccggttcgcc gcgccggctg gctccggaga
    19321 ctgaggtgac tctgttgcgg gtggcgcagg aggcgctcgc taacgtccgt aagcacgccg
    19381 ccgcgagccg cgtagtcctg acactctcct acatggagga ccgtgtcacg ctcgacgtgc
    19441 aggacgatgg tgtgggcttc gagacggccg cctcgcgaaa cggctcggga agtagtgtga
    19501 acggagatgg gctgggcggg tttggtctgc gcgcgatgcg cgagcgggtc gagcagaacg
    19561 gcggcaggct attagtcgag agcgaacccg gacgagggac cgcgctagtg gctgagttgc
    19621 cgctcgccgg tggagttagc cagggcgcgc gggcctcaca ggaggcgcga tgatgggacg
    19681 gataaagctc ctcatcgccg acgaccatcc cgtggtgcgg atgggtttgc gggggatgct
    19741 cgacggccag gaagacttcg aggtcgtcgg ggaggtcgag aacggcgcgc aggccgtcga
    19801 ggcgacggag aggctgcggc ctgacgtggt cctgatggac ctgcggatgc cggagatgga
    19861 cggtgtcgcg gccaccacca ggatcgtgga gcggacgccc gaagcgcgcg tgctggtgct
    19921 tacaacctcg gactccggcg cggacatact acgggccgtc gagatagggg cgaccggcta
    19981 catcctgaaa gacgcgccgc gcgaggaact gttccgggcg gtgcgcgcgg cggccgaggg
    20041 caagccgcta cttgcgccgg acgtggcggc gcacctgatg aaccgggtca ggtggccgtc
    20101 ggacgaggcg ttgagcagcc gcgagatcga ggtcctggaa ctcgtggccc aggggacgag
    20161 caaccggaac atcgccggga agctctggat aagcgaagcg accgtaaagt cccacctttt
    20221 gcacatctac ggcaaactgg gcgcgagcga ccgcgcctcg gccgtctcgg tggcgatgaa
    20281 gcgcggcatc ctgcgctcgt agctggtgga gaggatctag ccgtggcgtt cgagggcgag
    20341 ctgtatgaga cggtcgagga gctgttcgta ggggaggccg ctcgcgtccc agagataggc
    20401 gtagacgctg gtcggggtga agccggggat cgtgttgatc tcgttcagta gcagacggtc
    20461 cgtgccgcgt tcgaggaaga agtccacgcg ggacatgccg gcggcgtcca cggattcgta
    20521 ggcgatccgg gctacgcgcc gcatctcgga ggcgatctcg tccggtatgt cggcgggggc
    20581 gacgatcttg ctgccaccct cggtgtactt ggcctcgtag tcgtagaact cgttggccgt
    20641 aaccaggacc tcacccggca ccgagacttc cgggtcttcg ttgccgagca cggatacctc
    20701 tatctccctc gcatccaccc cggcctcgac tatgatgcgc cggtcgtgtt cggcggcaac
    20761 gtcgagggcg tttcgaaggc tcccggcatc cgtcgcgcgg ctgatcccga cgctggaccc
    20821 gaggttggcg ggtttgacga agcacgggta gccaatggaa tcctctatct ggcgcaggat
    20881 ggatttactg tctctctccc agtttgtgcg cgtgaggccg agccactcta cctgcgggag
    20941 accgtggtgg gcgaagattt ttttcatcgt gatcttgtcc atgctcgcag cgctcgcgag
    21001 cactccgctc ccgacgtaag ggacgcccgc aagctccagc atcccctgaa tggtcccgtc
    21061 ctcgccgtag gggccgtgca agaccgggaa gaccacgtcc accgacccga ggttcgccgg
    21121 tagcttctcc gagatggaag caggagagtc ggagatcgga gattcggaac cgttggcgct
    21181 gtcgagggcc ttgcttgcgg actccagctc ccgcatgggg tcgccggagg agatccaacg
    21241 tccttcccgc gttatgccaa cgggcactac ctcgtaccgg ccgctttttt cgagggcatc
    21301 ggtgatggcg cgagcggagg cgagggaaac atcgtgctcc ccacttcgcc cgccgaagat
    21361 gaccatgacc cgcactaaag aaactcctct cgccgccagc agcccatcgt agcagagagt
    21421 gtatttggga tcaggccgcg aaccccaggg agccggccct ctcgcgcagc gctgcggcga
    21481 cgaactctat gtgctcgttg aggtccacgc cgagttcctc cgctccgcgc gtgatgtcct
    21541 cgcggctgac ggcggcggcg aaggatttct gcttcatctt cttgcggacg ctcttggcct
    21601 tcatgccctc aaatccctcg ggacgcatga gggcgcaggc gacgatgaag ccggagagct
    21661 cgtccacggc gtagagggtc ttggacatga gggtgtcgcg gggtacgtcc aggtagtccg
    21721 cgtggccctt tatggcgtgc aacacgtcgt ccgggtagcc gagccgctcc agctcccgga
    21781 cgccgaccat cggatgctcg gccggggtgg gatgcttctc gtagtccatg tcgtgcagga
    21841 ggccggtgac ggcccacttc tcctcgtcct cgccgaagtg gcgggcgtag gcgcgcatgg
    21901 cggcttcgac ggcgagcatg tgctttcgca aggaatcgct ctcggtccac tcgcacatca
    21961 ggttccaggc atcgtcgcgg ctcatcgggt tcatggcatc tccttgtacg ttggttcgag
    22021 ggactattgt aggcctatgg ccgagaaacg tgcaaaatgg cgtcattccg tgtcaatcga
    22081 gtcttataga caggcgctac ccggtcaatt ccggctgtca tgtttttcgc tctgcccatc
    22141 cgccagcatg gatggatcta ctttaaatcg caagcaatcc agtatacgtt gacgacgaca
    22201 caggcgaggc ggaagacagc atgggtagcg cgaccgaaga gcgtccggcg gcactgtttt
    22261 aacgtctctc cgctgaacct tttgtagtgt tattccggga ggtcttatcc gctggagctg
    22321 taggagatct tgatctcgcc gccgcgggtg ttggtctgct ggacggagcg ggtggtattg
    22381 ccggcggtca ggatgacctt gaggctgccg ttgcccgcgt ctcgcttctg gatgctgcac
    22441 tggagctcat cacccgccag gtcgtaagcg taacgcttgg gcaccttgcc cttgaggacg
    22501 gtctcttgct tgccgtcggt gcagcgaccg gagaagcgcg tcttcgggtc cccttcgagc
    22561 cgcagaacga cgtccggctc ctccttgccc gcgccgaggg tggtctcctg tacggtggcg
    22621 tttgtggtgg tctcatcttg gctggactct tcggagatcg tgggctcctc gaaagcggtc
    22681 gtctgtttgc cggaacctcc ggtgctgccg gaaccctcgt cggtgtcgcc gccggtggag
    22741 cagccggcgg cgagcaggag cgcggccacc aggagaacga gaaagcttgt tcttcgaggt
    22801 tttatggtct tgccgggcga gtcttcacga tagtgtcatt ctatcaatcg gagacgatga
    22861 gggaggactg ccgcgccgat agttgccggg cgcgctggag caccgagtcg agcatcgggg
    22921 gcgtgagcac gccggtgaag gtgttctgct gcgaggggtg gtagcagccg agcagggtca
    22981 tgccgtctgc ctcatactcg gcgccgtgac cgaactttgg tttgggacgc actttgtaga
    23041 ggcgcagggc ggcgtcccag gcgaaggctc cgaggcagac gatgactttc gcgccgatga
    23101 gttccaactc gcgggcggag taggggaggc aggtgtcgcg ttcggcgggg gtcggtttgt
    23161 tctgtggcgg ggcgcaccgg acggcggcgg agatccaggc tccagtgagc cgcatcccgt
    23221 cgtcccggcc gcgcgagacc ggctggttgg cgaagccggt acggtgcagg gccgcgaaga
    23281 ggaagtcgcc cgagcggtcg ccggtgaaga cgcgcccggt gcggttcgcg ccgtgcgcgg
    23341 ccggcgcgag gccgagcagg acgacgcgcg ccgccggatc gccgaagccg gggatcggcc
    23401 gcgcccagta gtcctcgacg gcgtacgcgg cgcgcttgac gcgcgcgacc tcctcgcgcc
    23461 acgcgacgag gcgcgggcag cgccggcagg tggtgatctc ggcttcgagc gccgcgaggg
    23521 cgcgggcgct cacgagggag cgggcgtgcc ctggtcgagc aggccgcgga cgaaccgggc
    23581 gatgaccggc tcgagctcct ggatgttcgc cgggcggccg tggcgcagct ggcgcgaggc
    23641 gagctcgacg accgcgccga cgagcgcgaa cgcgtcgtcg ggggaggcga agcgcggcgc
    23701 gccgtagcgc tcgtgtgcga tcgcgttctg ctcgtcgagc atctgcgcga aggcctcgag
    23761 cacggcgtcg cggcgggcgt tcgcccgggg cccggcgccg atgatctcga c
//
LOCUS       DASSMB010000263         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1724397, whole genome shotgun sequence.
ACCESSION   DASSMB010000263 DASSMB010000000
VERSION     DASSMB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1724397"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VFJ72_15115"
     CDS             complement(<1..594)
                     /locus_tag="VFJ72_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480834.1"
                     /translation="MAESIGVDGVVERSPIRKVAFASAIGATIEWYDFFLYGTAAGLV
                     FNQLFFPSGNPTAGTLAAYATFAAGFVARPVGGLIFGHFGDRIGRKKMLVLTLLIMGI
                     ATFAIGLLPTYAQVGIWAPALLLALRLVQGIGLGGEWGGAVLMAVEHAPEGRRGFFGS
                     WPQVGVPAGLLLGTGVFTLLSVGLTDAQFLTWGWRAAF"
     gene            973..2064
                     /locus_tag="VFJ72_15120"
     CDS             973..2064
                     /locus_tag="VFJ72_15120"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7480835.1"
                     /translation="MVNRERSGRGPGGEEGLSVVDVDVHEMLTSIQDLVPYLDEPWRS
                     RVAINDGFKGPPGNPYAFPQATGVAIADAVTDDGSPAGSKLELLQEQVLDRYNIDPAV
                     LVSLFHPTDHQIQPEFAVALASAYNDWIVENWLERDDRLRSSITVAAQEPLAAAREID
                     RVGSDPRFVQVILPAVAHHVFGREFYWPIFEAAQRHNLVVAFHQSGGTRTAVGLPPYY
                     IEWHTAISQAWQSQLIGLIAHGVFDRFEELKVAMVESSWTWVPHLMWRFDYNYRSLRR
                     EVPWVKRLPSEYIRERVKFSTQPMEYPEDPQELYRMFEMIHSEDFLMFATDYPHWDFD
                     SPEQALPSTFPKDLRRKILTQNARDFYDF"
     gene            2084..>2286
                     /locus_tag="VFJ72_15125"
     CDS             2084..>2286
                     /locus_tag="VFJ72_15125"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET7480836.1"
                     /translation="MREIVREGLRETPSGRGEQRHVLCGRDDIPPGSVEGFRVGGRRI
                     ALARLDDGTFRVVADTCPHQAAQL"
BASE COUNT          459 a          695 c          739 g          393 t
ORIGIN      
        1 gaaggccgcc cgccagcccc aggtgaggaa ctgcgcgtcg gtaagcccca cggagagcaa
       61 cgtgaatacg ccggtcccga gcaggagtcc ggccggcacc ccgacctgcg gccagctacc
      121 gaagaagccc cgccgtccct cgggcgcgtg ctccacggcc ataaggaccg cgccgcccca
      181 ctcgccgccg agtccgatgc cctgcaccaa ccgcagcgcg agcagcaggg ctggagccca
      241 gataccgacc tgcgcgtagg tcggcagcag cccgatggcg aaggtggcta tgcccatgat
      301 gaggagcgtc aacacgagca tcttcttgcg accgattctg tccccgaagt gcccgaagat
      361 gagtccgccc accggccgcg ccacgaaccc cgcggcgaac gtcgcatacg ccgccagcgt
      421 cccggccgtc gggttgccgc tcgggaagaa tagctggttg aacaccagcc cggccgccgt
      481 cccgtacagg aagaagtcgt accactcgat ggtcgcccct atggcgctcg cgaaggcaac
      541 cttccggatc ggcgaccgct caaccacccc gtctacgcca atactctcag ccaagccaac
      601 acacctttct cgaatactgc actttttcta ctaatccaat gtacaagatg cggaagatac
      661 acctgaggga tggagaggtc aagacgtcgg acgtaaattt ttcgtaaacc ttctaaagct
      721 tggacagaga tgaagtggcc tctggcggga ctctggcagg cctagaaggc ccgactgccg
      781 aaaatatttc ggcggcgagg gatctctgat tttcggcttg cgaatgcgga taaacgttac
      841 cgcaacagga atcggtgttg taacctggat acgtttctcc cttgactctt tgcagcattc
      901 ccgttagcgt ttgtattgag aagtaaaaag gtagacaaag ttaagttaaa gggaggttaa
      961 gcgttgactg atgtggtgaa cagggagcgt tcggggagag ggcctggtgg ggaggagggg
     1021 ctctccgtgg tggacgtgga cgtgcatgag atgcttactt cgatccagga cctggtccct
     1081 tacctggacg agccgtggcg gagccgggtg gcgatcaacg acggtttcaa ggggcctccg
     1141 ggcaacccgt atgcgttccc gcaggcgacg ggcgtggcga tagcggacgc cgtgacggac
     1201 gacgggagtc cggcggggtc caagctggag ttgttgcagg aacaagtgct cgaccggtac
     1261 aacatagatc cggcggtgct ggtgagtctg ttccatccga cggaccatca gatccaaccg
     1321 gagttcgccg tcgccctcgc ttccgcctac aacgattgga tcgtcgagaa ctggctggag
     1381 agggacgacc gtttgcgaag ctccatcacc gtcgcggctc aagaaccgct cgcggcggcc
     1441 cgcgagatag accgggtggg gagtgacccg cggttcgtgc aggtaatact gccggcggtc
     1501 gcccatcacg tgttcgggcg cgagttctac tggccgatct tcgaggccgc ccagaggcac
     1561 aacctggtgg tggctttcca ccagagcggt ggcacgagga cggcggtggg gctgccgccg
     1621 tactacatcg agtggcacac ggccatcagc caggcgtggc agtcgcagct aatcgggctc
     1681 atcgcccacg gcgtcttcga caggttcgag gagctgaagg tggcgatggt cgagtccagc
     1741 tggacatggg ttccgcacct catgtggcgc ttcgactaca actaccgctc tttgaggcgc
     1801 gaggtcccgt gggtcaagcg gctgccgagc gagtacatcc gggagcgggt caagttctcg
     1861 acgcagccga tggagtaccc ggaggacccg caggagctgt accggatgtt cgagatgatc
     1921 cactccgaag acttcctgat gttcgccacg gactacccgc actgggactt cgactcgccg
     1981 gagcaggccc tgccgagtac cttcccgaaa gacctgcggc gcaagatcct cacccagaac
     2041 gcccgcgact tctatgactt ctagggagac cgttcgggaa tccgtgcgag agatcgtccg
     2101 cgaagggctg agggagacgc cttccggacg cggagagcaa aggcacgtcc tctgcggacg
     2161 ggacgacata ccacccggaa gcgtggaggg tttccgggta ggcggacgga ggatagcgct
     2221 cgcccgcctg gacgacggta ctttccgcgt cgtggccgac acctgtccgc accaggcggc
     2281 gcagct
//
LOCUS       DASSMB010000264         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_98658, whole genome shotgun sequence.
ACCESSION   DASSMB010000264 DASSMB010000000
VERSION     DASSMB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98658"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..2374)
                     /gene="hypF"
                     /locus_tag="VFJ72_15130"
     CDS             complement(47..2374)
                     /gene="hypF"
                     /locus_tag="VFJ72_15130"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET7480837.1"
                     /translation="MRIPTRERREVSVRGIVQGVGFRPFVYALARKHGLTGLVRNDAE
                     GVRIEAEGTPEELERFVRALEEDAPPLAVVESVEWKPLPALGDREFRIEESREGLRRR
                     ALVSPDVATCDDCLAELLDPADRRYRYPFTNCTNCGPRFTITRQVPYDRATTTMCGFT
                     MCPECLREYDDPADRRFHAQPNACPVCGPRVRLVDKFGSELRDKREDPIARTARMLRG
                     RAVVAIKGLGGYHLACDPFDEKAVRILRGRKVRQDKPFAIMARDLAQARDLCRISPQE
                     EALLTSPARPIVLLERKEDSGVAEEVAPRQKTLGLMLAYTPLHHLLLRDAGIPLVMTS
                     GNNSDEPIAYRDGEAFDQLCEIADYFLVHDREIHMRCDDSVVRAAGGRPYPIRRSRGY
                     APAPLRLAGGFSRHTLACGGELKNTFCLAKDRHAFLSHHIGDMENYETLRSFREGIEH
                     YSRLFDVQPELVAYDLHPEYLSTKYARELEEAGLPAVGIQHHHAHVASCLADNERPAE
                     ERVIGVALDGTGYGTDGAVWGGEFFEGSVEEGFTRRAHLEYAPLPGGSAAIKQPWRMA
                     LAYLISLHGEEETLNLPLAVVREAGERNARLIARLVQHKLNTPPTSSAGRLFDAVAAL
                     AGVPGSQRTTYEGQAAIELELAATGPTNRAYPVQLRTEGDGWVVETRQIVAAVVEDLL
                     AGRPAAEVSATFHRTMAEIVTSVCEAVREAGGAEAVALSGGTFQNMLLLNQMVGLLEE
                     KGFVVHVHRRVPANDGGISLGQAVLADRVMREREA"
     gene            complement(2551..3063)
                     /locus_tag="VFJ72_15135"
     CDS             complement(2551..3063)
                     /locus_tag="VFJ72_15135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HET7480838.1"
                     /translation="MSKRVLVAGVGNIFLGDDGFGVEVAKRLAGRELPEGVEVKDFGI
                     RGMDLAYALHDDYDLVVFVDATPRGGEPGTVYLIEPEVEEDGELSLDTHGMDPVKVIK
                     LSRALGARPTRTLVVGCEPGVILSGEDYDDMLMELSGPVRAAVDEAARLVESLAEEVC
                     ETNGEREVRS"
BASE COUNT          513 a         1146 c          959 g          566 t
ORIGIN      
        1 catccagtat ctccacgatc cgtcccggaa ttcccaagca cacgtcctat gcctccctct
       61 ctctcataac cctatccgcc agtaccgcct gtcccaatga gattccaccg tcgttggccg
      121 gtacgcgtcg gtgtacgtgg accacgaacc ccttctcctc taataatccg accatctggt
      181 tcagtaacaa catgttctgg aacgtgccgc ccgagagagc gactgcttct gctccacccg
      241 cctcccgcac cgcctcgcag accgaggtta ctatctccgc catcgtccgg tgaaaggtgg
      301 ccgaaacctc cgccgccggt cgtccggcca gcaagtcttc gaccactgcg gctactatct
      361 gacgagtctc gactacccac ccgtcgccct ccgtccgtag ctggacgggg tacgcccggt
      421 tcgtcggacc ggtcgcggca agctccagct ctatcgccgc ctgcccttcg taagtcgtgc
      481 gctgcgagcc cggcacgccg gcgagtgcgg ccacggcgtc gaagagccgg ccggcgctgg
      541 atgttggcgg ggtgttcagc ttgtgttgca cgagccgggc gatgagcctc gcgtttcgtt
      601 cgcccgcttc ccgcactacc gcgagcggaa ggttgagggt ctcttcctcg ccgtggagcg
      661 agatcaggta cgcgagcgcc atgcgccagg gctgcttgat cgccgccgat cctccaggca
      721 agggagcgta ctccaggtgc gcccggcgcg tgaacccttc ttccacgctg ccctcgaaga
      781 actccccgcc ccagacggcg ccgtcggtcc cgtagccagt cccgtcgaga gctaccccga
      841 tcacacgctc ttccgcagga cgttcgttgt ccgcgaggca acttgctacg tgcgcgtggt
      901 ggtgctggat accgaccgcc ggaagtcccg cttcctccaa ttcgcgggcg tacttggtgg
      961 agaggtattc ggggtgcagg tcataggcga cgagttccgg ctgtacatcg aagagcctgc
     1021 tgtagtgctc gatcccctcc cgaaacgacc gcagcgtctc gtagttctcc atgtccccga
     1081 tgtgatggct caggaaggcg tgccggtcct tcgccagaca gaaggtgttc ttcaactctc
     1141 caccgcaggc caacgtgtgc cgggagaagc ccccggccag ccgcagcggg gcgggggcgt
     1201 agccgcggga gcggcgtatc gggtacggtc tccccccggc ggcccggact acactgtcgt
     1261 cgcagcgcat gtggatttcg cggtcgtgga ccaggaagta gtccgcgatc tcgcagagct
     1321 gatcgaacgc ctccccatcc cggtaggcga tgggctcgtc cgagttgttg ccgctggtca
     1381 tcaccagcgg gatgcccgcg tcgcgcagca gcaggtggtg cagcggcgtg taggccagca
     1441 tcaagccgag cgtcttctgg cgcggggcga cctcctcggc caccccgctg tcttccttac
     1501 gctccagcag cactatcgga cgggccggcg aggtgagcag cgcttcttct tggggcgaaa
     1561 tccggcacag atcacgggcc tgcgcgagat ctcgcgccat gatcgcgaag ggcttgtcct
     1621 ggcgcacctt acggccccga agaatcctca ccgccttctc gtcgaacggg tcgcaggcca
     1681 ggtggtagcc gccgagcccc tttatggcga cgacggcccg gccgcgcagc atccgggcgg
     1741 tccgcgcgat ggggtcctcc ctcttgtccc gcagctcgct gccgaacttg tccacgagcc
     1801 gcacccgcgg cccgcagacc gggcaggcgt tgggctgggc gtggaagcgc ctatcggcgg
     1861 ggtcgtcgta ctcccgcagg cactcgggac acatcgtaaa gccgcacatc gtagtcgtgg
     1921 cccggtcgta ggggacctgc cgagtgatgg tgaagcgcgg gccgcagttg gtgcagttag
     1981 tgaatgggta gcggtaacga cggtccgccg ggtcgagtag ctcggcgagg cagtcgtcgc
     2041 aggtggcgac gtccggggag acgagagccc gccgacggag cccctcccgg ctctcctcga
     2101 tgcgaaactc ccggtccccg agcgccggaa gcggcttcca ctcgacggac tcgaccacgg
     2161 ccagcggtgg ggcgtcctcc tccagcgcgc gcacgaagcg ctctagctcc tccggcgtgc
     2221 cttccgcctc tatgcggaca ccctcggcgt cgttacggac caatccggtg agcccatgct
     2281 tgcgcgccag ggcatagacg aacgggcgga agccaacccc ctgcacgatt ccccgcacgg
     2341 agacctcgcg gcgctcgcgc gtaggaattc tcacggcgtc aaccttcgca atcgcgcgga
     2401 agtccaaaca atttctctct tcaccggtgc tctcaaccag ctcgcttccc cggacctaca
     2461 tgttccgcat ctttatgtaa cgttggatct ccggccactg cgcgaccact gcggcaacac
     2521 ccgcggccac gcccaggagc aatagtttcc tcacgatctc acctcccttt ctccgtttgt
     2581 ctcacagacc tcctctgcca gcgactccac cagcctcgcg gcctcgtcca ccgccgcgcg
     2641 caccggcccg ctaagctcca tcagcatgtc gtcgtagtct tcaccgctca ggatcacccc
     2701 cggctcgcaa cccactacca gcgtccgggt cggacgggcg cccagagcgc gggacagctt
     2761 gatcaccttg accgggtcca tcccgtgcgt gtccagcgac aactccccgt cctcctcgac
     2821 ctccggctct atgaggtaga cggtgcccgg ctcccctccc cgcggcgtcg cgtccacgaa
     2881 gaccacgagg tcgtagtcgt cgtgcagagc gtaggcgagg tccatcccgc ggataccgaa
     2941 gtcctttacc tcgacaccct ccggcaactc gcgcccggcg agccgcttcg ccacctcgac
     3001 cccgaaaccg tcgtcgccca aaaatatatt gccgacacca gcgaccagaa cgcgcttact
     3061 catcggcggt cccggtgtcg tccagcggca cgacctcctc gggggagaag aagaagcggt
     3121 gtccgaggat gtgcatatct cccaggtccc tgccggggtc gtcctccagc gtgacggaca
     3181 ggtg
//
LOCUS       DASSMB010000265        25915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1353946, whole genome shotgun sequence.
ACCESSION   DASSMB010000265 DASSMB010000000
VERSION     DASSMB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 25915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25915
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353946"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1057)
                     /gene="mraY"
                     /locus_tag="VFJ72_15140"
     CDS             complement(59..1057)
                     /gene="mraY"
                     /locus_tag="VFJ72_15140"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7480839.1"
                     /translation="MFSVILGAAVAFIVTVALGSKFIEFLQTRKFGQYVREEGPQTHL
                     IKAGTPTMGGVVMLMGLLAALVVVARPNVATFTTLLLVGSVAVVGMYDDWQKISRRSS
                     EGLSARYKFLLLTLTVVVADVLALRYVGVTQNVIVPGIKRNLVLGPGFLGVALFSIFL
                     LLVIAGTTNAVNLTDGLDGLAAGAGGIALLAYTAIAFLERQYDVAIICGAMVGAIIGF
                     LWYNSHPAEIFMGDTGSLAIGGVLAAAAVLTKTEMLLPVIGGLFVLEALSVMIQYGVF
                     RLSGRKKRVFKMAPIHHHFEMSGWEENKVVVRFWIVQSAFAALGFVLYYFYLYNAV"
     gene            complement(1050..2423)
                     /gene="murF"
                     /locus_tag="VFJ72_15145"
     CDS             complement(1050..2423)
                     /gene="murF"
                     /locus_tag="VFJ72_15145"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET7480840.1"
                     /translation="MRAASLGQIAEVLGATFKGCDPETLAHGAATDSRIVRDGDLFFA
                     LRGATDGADFASEAHLRGAVATVADRALDVPTLVVEDALAALQQLARWSLRREDPPVV
                     VGITGSVGKTTTKDALAAILRVAGRRVSATAGNFNNEIGLPLTILAANERTGILVLEM
                     GATHRGDIEHLCSIAPPQVGVLTAIAPVHLDSFGSLEGVAEAKGELALALPESGSFVS
                     PHGAPDAATGYRRNLARRIRFSRDHAEGVDLWISGLEERDEGLRFVVHLRDEEAEVRC
                     PVFGTHLVEPLLAAIGGALALGLSLDECARGVSRVRRTGLRGEVFRLRDDIIVYDDSY
                     NASPVATAAVLRYGEEGSREQGRRLVAVLGGMFELGAGARAYHHEIGELAEELRVDLL
                     VCVGDEARWYAETFSGETLMFPDAESAAEGLREALRPRDYVVVKGSRGVRLDALTRKL
                     RESLALV"
     gene            complement(2420..4015)
                     /locus_tag="VFJ72_15150"
     CDS             complement(2420..4015)
                     /locus_tag="VFJ72_15150"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7480841.1"
                     /translation="MRAVVAAVTVASLLLTGRAMHLSFADGERYQAFAAEQDAQQRQV
                     SPQGRGSIVSADGRKLAVSLEASTIVATPYQIEAPKNAAGDLAQILKSETGQDEREIE
                     SKLTQKDSRGHLSGYSVLGTVGPEAAEKVRNLGIEGITLVPGSDRVYPDGELAGQLTG
                     YLSDHGKAFGGVEARYDKRLKSGQDVRVTIDTAVQEELQTALVSAVKEYHAKSALGVV
                     MRVDDGAVVAVANEPGYDNNLYTEASADVQRDRVLTDPYEPGSTFKPFTIASALEEGT
                     LTPDDTFTVADQIQVADRVVHDSEPHPTEVMYPENILQRSSNVGTIQIAEGLGGQLLY
                     QYIRRFGFGDRTGVDLWGEDPGSVPAYEDWSGSSIGNIPMGQGLTVTPLQLAAGYAAL
                     ANGGLKVTPYVAQDAAPKGPGPRVISAKTSDIVRGMLQSVVDSGTGRFAQIPGYTVAG
                     KTGTSQKVDPVTGTYGEEYVSSFVGFAPASRPEYVTLIAVDEPQGLIWGERVAAPAFE
                     KVMSFTLGYFNVVPDAPEESGSE"
     gene            complement(4158..4601)
                     /locus_tag="VFJ72_15155"
     CDS             complement(4158..4601)
                     /locus_tag="VFJ72_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480842.1"
                     /translation="MAVPRPGRRDRIYESGVFVPPIRERERLHVDRAHQAKVRRKEIE
                     RRRGFAALVVIPVLLMLGSIYLHTVSGSLGERVAGLEERVARAEADGQKLNVQVAELS
                     APGRIREKARDLGLSEPGGEDMRVDGGNGEDGKAYAGEKQREENP"
     gene            complement(4601..5548)
                     /gene="rsmH"
                     /locus_tag="VFJ72_15160"
     CDS             complement(4601..5548)
                     /gene="rsmH"
                     /locus_tag="VFJ72_15160"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET7480843.1"
                     /translation="MMVAGHRPVMLEEAVAALAVSDGDVVVDATFGGGGHSRRILEKL
                     APDGRVIGIDRDPEAAGRASDLLRDRRFSFRAGEFGDVLRTLVEEGAWVDAVLFDLGL
                     SSFQIDAAGRGFSYVNEGPLDMRMDHGSGVSAAEFLDTVTESELVRILINYGDVPRPE
                     ARRIAREVLRHRPLRTTKDLADAVRAAVGWKERGGNPAKRVFQAVRVHVNDEVRGLER
                     ALRAAERLLVAGGRLVVISFHSGEDRIVKRFISEREGRCVCPPDLPVCVCGARPIFRR
                     GAAIKPTQEEVEANPRSASARLRVAIRTAEPLNDHGDRD"
     gene            complement(5545..5985)
                     /gene="mraZ"
                     /locus_tag="VFJ72_15165"
     CDS             complement(5545..5985)
                     /gene="mraZ"
                     /locus_tag="VFJ72_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET7480844.1"
                     /translation="MALLGEYEHTLDEKGRLTLPSRLRSYFEGGIVVTKGVDRCLFAF
                     PPDEWAAFKANIKANADLTAKGRQLSRMFFSMAFEATLDRQGRVLIPTKLGQYAGLRR
                     DVTIAGVDDRLEIWDTEEWDRYVTGADESFAEIVEEFAGREFGG"
     gene            complement(6355..6428)
                     /locus_tag="VFJ72_15170"
     tRNA            complement(6355..6428)
                     /locus_tag="VFJ72_15170"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6392..6394),aa:Ile,seq:cat)
     gene            complement(6475..6547)
                     /locus_tag="VFJ72_15175"
     tRNA            complement(6475..6547)
                     /locus_tag="VFJ72_15175"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6512..6514),aa:Asn,seq:gtt)
     gene            complement(6587..8368)
                     /gene="dnaG"
                     /locus_tag="VFJ72_15180"
     CDS             complement(6587..8368)
                     /gene="dnaG"
                     /locus_tag="VFJ72_15180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET7480845.1"
                     /translation="MRISQRSIETIRETSNIVEVASEFTALRRQGARFSGLCPYPDHQ
                     EKTPSFSVTPEQGFYYCFGCQRGGDAIKLVTDLKSLSFAEAVSYLAERSGTELEFEGG
                     SEADSKAARDRTLRRRKIHKALAAAAIYYHKYLLKSQTPQAEESREYLKQRGLVLSTI
                     EEFRLGYAPPRGSSGFSGVAGRFELDRRILDAAGILSSRGGERFAGRITFPISDRRGR
                     MVGFGARALGDDHPKYLNSPETDVFNKRELLYGFPQVAEAIRRERAALVVEGYTDVLM
                     LYQSGIKNAVATLGTATTPAHLKTLSGYADTIYLLFDPDTAGEKAIERAAVTASELKL
                     DLRVVRLSEDPADWLLKHQPEEFTDLLTAATSVLEYIFRRKADQARGSDAAERARTVS
                     EIRDLLRDIDDPVFYRDAIRVATEALGVSPEALQGSNGPDGRRDARTRPSTVQSAAQS
                     PILRAGREVLALILTRPDLTSRPLREGVSAAEIPEPVMLGAPDFADEAQARLFTLLSE
                     HAGDDLDAVLADDRARDLMEQIAALYAEGEKLYPSEAVVRESWLRLAILSRQRAKQDA
                     PDLDAKERLHSEIQRLKEALRAVSSEP"
     gene            8449..11268
                     /gene="ppc"
                     /locus_tag="VFJ72_15185"
     CDS             8449..11268
                     /gene="ppc"
                     /locus_tag="VFJ72_15185"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7480846.1"
                     /translation="MNISRYLDQKSDNEYANLPPGVEFEKKDEPLRRDINLLGRILGQ
                     VLVEQEGADLLDAEEDLRLLCKRLRFDYDPELDERLRRRIEKMDAGELGRIVRAFSVY
                     FQLVNIAERYHRVRRRRQYETSPDNPPQRASLASALSRLAADGMDAAQIQETIDDLNV
                     GLVLTAHPTEALRRSVRRKHERIGEMLEQIESGLTTWKERQRLEEKLAEETTILWQTE
                     ELRSRRPAVSQEIQRTLLFFEHPLIDATLDVYREFENELANRFPDSALEVGRVLEFGS
                     WVGGDQDGNPFVKPETLTTALGLHREVILKRHISAVLALADHMSQSSKLVEVSEELAA
                     SVERASVSQPADVERHRGEDTNEIYYRKLTIIAERLRLTLDSPGSRDAYSGVSEFLDD
                     LMMIRRSLIGRGGERVADGGLRDLIRQAEVFGFHLAKLDVRQESSRVMEAAGALISPH
                     EGKDLADMDEEERAALLRDLAGDPDLDLPQPDGVTEEVRDVLETFERIRRAKDEFSEP
                     PVETFILSMARQASDVLSVQLLARRVGLLEVDSEGRCEASYLRITPLFETIEDLERAP
                     EVLRRLLDDSFYRSSLAKSGDVQEIMLGYSDSGKDAGYVTSNWTLYKTQSRLTDVAHE
                     YGVKLRLFHGRGGSAGRGGGPSYNAIMAQPPGTLDGSIRITEQGEVISFKYSMRGLAR
                     RNLDTVLAAVLEATVTQSHEDLAPEWTSVLDEISGRASETYRALVYEDEDFLPFFSGA
                     TPIEEISLLNMGSRPARRVGKPDVSSLRAIPWVFAWTQNRFLLPSWYGAGTALGNYIQ
                     QPDGLATLRDMYRRWPFFRTLTDFMQMTLAKSDLRIARTYTSLVEDAGVRERLWQRIA
                     EEHAACSEAILLITENDNLLDDSPVLQRSIRLRNPYVDPLSYIQVSLLRRLRSLPEDS
                     PERAGVLEALLLTVSGISSGLLNTG"
     gene            complement(11272..12225)
                     /locus_tag="VFJ72_15190"
     CDS             complement(11272..12225)
                     /locus_tag="VFJ72_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HET7480847.1"
                     /translation="MTTLRSRACEKSAGRVKPEPPDGVRNEFQRDRDRIIHAKAFRRL
                     MHKTQVFIAPDGDHYRTRLTHTLEMMQISRTIARALELNEDLAEAVAFGHDLGHTPFG
                     HTGEEALARALAPHGRSFRHNEHSLRVVDKLEKGGKGLNLTLEVRDGILNHTGKGHPA
                     TLEGEIVRIADRIAYVNHDVDDALRAGVIDFEDLPQAPLAILGREMSARIDTLVRDMI
                     STSEKQGEIALSDRVHVALMELRTWLFDNVYRNPDSRENRKAAGVVSSLFEYYLEHAE
                     ERANSDEDPISETIDFVAGMTDRYAIATYRRLFLPRGEIFA"
     gene            complement(12222..12980)
                     /gene="recO"
                     /locus_tag="VFJ72_15195"
     CDS             complement(12222..12980)
                     /gene="recO"
                     /locus_tag="VFJ72_15195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7480848.1"
                     /translation="MALYRSKGIVLRSIRYGEADRILDLYTQDAGLISAIAKGIRRTR
                     SRFGARLEPLSCVDFMAYGGRTLDTVTQVEVLRSFHVVREDLARFEAAGRMVQAVRAL
                     SGGDEADRRVFNLLYHALDSLESRDSGYRAVEAAFVLKLTILAGYAPHLDACLNCGAD
                     PEVSGPPYFAPGLGGVLCAECRAAASDTFPLAPQVLESLHALIERPVRDAMVQDGLEE
                     SVLRVVRAHLSAHAPGATVNTAAPLSDTGTRRPA"
     gene            complement(12961..13662)
                     /gene="deoC"
                     /locus_tag="VFJ72_15200"
     CDS             complement(12961..13662)
                     /gene="deoC"
                     /locus_tag="VFJ72_15200"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET7480849.1"
                     /translation="MRVREFAKTVDHTLLKPDATEQDIARLCEEAAHYHFAAVCIPPC
                     YVRFAAKQLRGVDVKVATVVSFPFGADTTSIKVQAVRDAIVGGASELDVVMNISRFLS
                     GDFNYVAEELVSLNQEVSVVAMNNGLNDVVVKVIVETAYLTDKMKRLATQIVAASGAD
                     FIKTSTGFAPSGATVEDVALLREEAPEGLAVKASGGIRTLDEALDILNAGASRLGMSA
                     SVAIMEEAEGGALQE"
     gene            complement(13712..14635)
                     /gene="era"
                     /locus_tag="VFJ72_15205"
     CDS             complement(13712..14635)
                     /gene="era"
                     /locus_tag="VFJ72_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET7480850.1"
                     /translation="MSQALDHALAEEDLRSGFVAVVGRPNVGKSTLVNRLVGKKVSIT
                     SPRPQTTRGPVRGVRNGPGYQAVFIDTPGSQKPRDTLRARMQQQVLDSLGESDVILFL
                     LDAAQAARGIGSGDRYVADLVARSGTPVLVGINKVDLLSRETDALPLVDEVSKLAGWK
                     DVFLLSAVTGRNVEPLMETVSRLLPLGPQYFPEGTVTDYPETLILGEYVREKALSVLR
                     EEVPHAVAVEIDEVERKENVTVVYAVIHVERATQRMIVLGKGGKTIKQIGTEARRDVE
                     RLLGTRIYLDLKVKVTPGWRSDPKFLERLGL"
     gene            complement(14632..15021)
                     /locus_tag="VFJ72_15210"
     CDS             complement(14632..15021)
                     /locus_tag="VFJ72_15210"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7480851.1"
                     /translation="MDRQDVMAAARSAACNAYAPYSNFHVGAAILTEGGKLFPGCNVE
                     NASYGLGICAERNAAAAMALSNPDDRSVRLVAVHSPNAAPCFPCGACRQVLREFGCKE
                     VVVEDADGLRSYPFEEILPNSFGPESL"
     gene            complement(15045..15806)
                     /locus_tag="VFJ72_15215"
     CDS             complement(15045..15806)
                     /locus_tag="VFJ72_15215"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET7480852.1"
                     /translation="MSEQDRRLEEPRPAEQPKKGQQGVARSFNHAYRGLIFAVRTQRN
                     MRFHVVAAVLVLVLSLLVQVSALELAALVIVILVVLVTEMFNTAIEFTVDLATREYHP
                     LAKLAKDVSAGAVLVTSIGAVLVGYLILADNLGPLSLNALQTVRAWPGHLTLVSLGVV
                     TLAVLLIKALTRAPNSFSGGMPSGHAAVSFAGWVAASFIAAEGRYGGLVSLITLLMAL
                     LVCQSRVESGTHDVYQVVAGALIGVLITVAIFQFL"
     gene            complement(15790..16206)
                     /gene="ybeY"
                     /locus_tag="VFJ72_15220"
     CDS             complement(15790..16206)
                     /gene="ybeY"
                     /locus_tag="VFJ72_15220"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET7480853.1"
                     /translation="MPFSVALLDEVSYGGLDAARVTDLCATAFSARGFDLERLGEVSV
                     ALVDPETIHELNREYRGVDRTTDVLSFEVDGPYGEMVGEVVIAPDCAHPEMGVEELVV
                     HGALHLSGMDHGDQFEDTEMARVQAAVMESARERAG"
     gene            complement(16221..18464)
                     /locus_tag="VFJ72_15225"
     CDS             complement(16221..18464)
                     /locus_tag="VFJ72_15225"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HET7480854.1"
                     /translation="MKLKEKRAALNGNRTDEFHRPPTRFERFRAWVERLPRMRLYVAI
                     TAVTWISLTGLIGLDDRPLTALGFSDNGQDYQVGAVSDADVYAPRSVTYTDPTATEEA
                     RQEAADAVAVVYRESSKVPADVEGNVKQFFDKVRKIRASDKPAEQKTQEISDASPSYL
                     PQTSARALVFVDDKELENIETYVTENLQEMYASTAVADDTVEEVPASVVRISEARNRL
                     SEAAVRDASGEEGALVEVLSREFVEPNYVVDRAATQKARDEAASKVGPVKGSIKQGER
                     ILARGEVIDRQAIAQLQALSIVRDTNPWTVILGVALVVAAEMGVAWYFLERFGKRILR
                     NNAMIRIVLAASLTILFTLLARGFVLLSLDPYLIPLAGLSIIGTILLGPRLMFLMVVI
                     SAVNVGIIGGNDFLLSAALLLTSGFAIYTVVRVTSRQQLLKAGLFIAVVMAGITFAVS
                     LVGGSDLGVALQQGALGLGNGLLSLMIAMMLLPLLEDAFNILTPMKLLELSDPGNELL
                     QKLLRTTPGTFSHSMQVGNLAENAAERIGADTLLARVGAYYHDVGKMEHPAYFIENQI
                     AQVNPHATLSPALSARVIKRHVKDGLEIGRMWGLPQEILDIIAQHHGTTRIEYFFRKA
                     RDLAPDPTTVRESEFRYSGGRPRSKEAGIVMIADSVEATVKSIAKPTPKRIEDVVSDT
                     IRHKLEDGQFDECELTMQEIHAVGEAILEALIGFLGPRIEYPEATPAEKKPPVGQIAG
                     KPAGSQT"
     gene            complement(18488..19399)
                     /locus_tag="VFJ72_15230"
     CDS             complement(18488..19399)
                     /locus_tag="VFJ72_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006681752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET7480855.1"
                     /translation="MLEVFGDRDTVLRRVEELVECEVIVRGNEIVLRGTEAAVERAET
                     VFNGLVGLAEDGHHPTPETAERLYRMGDGGEGRRVLGDVILTHRGRRVAPKTRNQKAY
                     TDAIREKPVVFGIGPAGTGKTYLAVALAVDALNRGEVSRIILTRPAVEAGESLGFLPG
                     DMMAKVDPYFRPLYDALYEMIDPGKFQGYLERGIIEIAPLAFMRGRTLNDAFIILDEA
                     QNTTPQQMKMFLTRLGFGSKMVVTGDVTQVDLPHGRRSGLEDARQTLTGVEGVKFVGL
                     KRDDIVRSDLVMRIVDAYEDAQGGSGA"
     gene            complement(19495..19938)
                     /locus_tag="VFJ72_15235"
     CDS             complement(19495..19938)
                     /locus_tag="VFJ72_15235"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET7480856.1"
                     /translation="MSIADQIQQDTKAAMKNRDRRRVETLRMLNAALKNGEIEAGRSL
                     SEEEEQAVLRRQLKQREESAEAFGKAGREEQAASEAAEAEVVREYLPAPLSEDELERI
                     VEQAIDETGASEMKDMGRVMGRATALAGGRADGRHLAALVRGRLQ"
     gene            complement(20005..20712)
                     /locus_tag="VFJ72_15240"
     CDS             complement(20005..20712)
                     /locus_tag="VFJ72_15240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HET7480857.1"
                     /translation="MDWTLIKGESFNLPEEESHHVLKVLRGRPGDAFEVVDGARCLFL
                     AELREGREAVVVESLGGEPAPGGEVILYQAVPKGRHMDLVVEKATELGAAGVVPLVTE
                     HGVVRLDERAAKLERWRRISESAARQSLQLRIPEVPEPVMFEEAVRRVGATGVILHNA
                     PDLPPLENCLREDEVSLFVGPEGGWSAGELRIATEAGLMAAQLGPYRLRSETAGIIAV
                     ARARAVLDRSDLGRARS"
     gene            complement(20751..21878)
                     /gene="dnaJ"
                     /locus_tag="VFJ72_15245"
     CDS             complement(20751..21878)
                     /gene="dnaJ"
                     /locus_tag="VFJ72_15245"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET7480858.1"
                     /translation="MATKRDYYEVLGLPREASEQEIKKAYRRLARDHHPDANPDDHGA
                     EERFKELTEAYEVLSNSESRRAYDTYGHQVPRGGGAYPGGDPFGGFQDIFEAFFGDRF
                     GGDPFFGSTGSRGPARGADTQVEVEVDLREAAFGADRDVKVQTVKNCSVCDGVGGTES
                     HVCGTCGGAGAVRTVRQSLLGQMVTTQACSACDGRGRVIDVYCDNCRGSGRVSEVESH
                     RLRVPPGIEEGMKLRISGGGHAGEPGAPPGDLYVVVKVKEDPELMRDGEDLIHRMRVN
                     VVEAALGTEVEVPTLNGGTEVKIEPGTQPGTTLKLRGEGMPRLRGRGRGDLKVVVDVM
                     VPTRLTAEQRELLERFGAVSGEETYNGSHGTFFDRLRGVFR"
     gene            complement(21881..22909)
                     /gene="hrcA"
                     /locus_tag="VFJ72_15250"
     CDS             complement(21881..22909)
                     /gene="hrcA"
                     /locus_tag="VFJ72_15250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7480859.1"
                     /translation="MRAFDIPDRQRDILTKTVEMYISTGAPVGSHTLSEEIDASSSTI
                     RAELADLESRGLLTHPHTSAGRVPTDAGYRLYVDALLAGKPPEETLFETPDGYGNVDE
                     LLRGVSEGMSEVTRLLAVVAGPSALGDSLARVDCLPLREGAFLLVVSMESGASASATI
                     SLPSSVNEEEMREILSSLNSWLGGRPVGTDLELGLAARKALSDHNPLVVGAILEAVAV
                     LGRATERGVFIQGASALLARLDDLDPASLAAVIEIFERRRWLLNLLGDALARSVSSSG
                     VVVSIGAENGFHNLTGTSLIAAAYNHRERPYGVVSLIGPKRMDYDAAISTVRSAADAL
                     THCLNTRF"
     gene            complement(22964..24742)
                     /gene="lepA"
                     /locus_tag="VFJ72_15255"
     CDS             complement(22964..24742)
                     /gene="lepA"
                     /locus_tag="VFJ72_15255"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET7480860.1"
                     /translation="MKNIERTRNFCIIAHIDHGKSTLADRLLDITDAVPERDRMAQIL
                     DTMDIERERGITIKAQAVRVLYKRDDGEWALNLIDTPGHVDFAYEVSRAIAACEGALL
                     IVDASQGIQAQTLANLYLAMEHDLEIIPVLNKIDLPIADVPAVTEEIVELLGVDEEDI
                     LKISAKTGEGVTDVLDAIVDRVPAPATEREETRALIFDSYYDPYRGVVSLARLFDGEL
                     KKGDKVRAMGSDEQFELLEVGCYSPKPTALPVLGTGEVGYIIAGLKDIEALRVGDTLT
                     TVEHPASEPLPGYAEALPTVFSGLYPTDGDDFERLRDALSKLQLNDASLFFEPENSRM
                     GFGFRCGFLGLLHMEIIQERLEREFDLDLIASSPSVRYEVVVNGETIEVTNPSDLPEI
                     FDEIREPVVRATIISPKEYVGAVMGLCHEKRGVSIGMEYISTRRVQLTYDLPLAEIIT
                     DFFDALKSRTKGYASYDYEPLGYEASDLVKVEVLVSGDPVDALSIIAHRDKAYARGRA
                     LTEKLKELIPRQQFEVPVQASVGKRIIARETVRAYRKDVIAKCYGGDISRKKKLLEQQ
                     KKGKRRMKQVGRVEIPQEAFLAALRI"
     gene            24832..25089
                     /gene="rpsT"
                     /locus_tag="VFJ72_15260"
     CDS             24832..25089
                     /gene="rpsT"
                     /locus_tag="VFJ72_15260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7480861.1"
                     /translation="MPAPSKRDKQSIKRGAQNKGVRTHLRNLSKNFYKALEAGDEDKA
                     REIRDQSQRAYSKAAGKGIIHANKASRKVSRFDKALATDRG"
     gene            complement(25153..>25915)
                     /gene="holA"
                     /locus_tag="VFJ72_15265"
     CDS             complement(25153..>25915)
                     /gene="holA"
                     /locus_tag="VFJ72_15265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET7480862.1"
                     /translation="TMVLLRNLDAWNAAQKNRVLGYVEDPSPDTDLVMLGKKLGAREK
                     LLAAVKKSGEVHDFAQPTGKALVKWVVGYARKQGLEISEDVAQDLTDRCSGDKVRSAR
                     EAEKLALYVENGAATLEDVDKLCLPDLQSNIFAFVDSLGSGDRGRAISLLEDLIGTGE
                     PALRVTYMMRRQFHLLARARALSEAGTPRSEIASQLKVPPFVARKLEDQSRRLDGGTL
                     EDALGIVLDLERGLKGGRDLADELQVEMAVMKLSR"
BASE COUNT         4698 a         8610 c         7851 g         4756 t
ORIGIN      
        1 cgtcgcagaa acccccgact ctcccaggcc gtatacgaga gtcctcacaa tcctggtctc
       61 acaccgcgtt gtagaggtag aagtagtaga ggacgaagcc cagagccgcg aaggcggact
      121 gcacgatcca gaaccgcacc acgaccttgt tctcctccca gccggacatc tcgaagtggt
      181 ggtgtatcgg cgccatcttg aacacgcgct tcttgcggcc gctgaggcgg aagacgccgt
      241 actggatcat cacggacaaa gcctccagga cgaagagacc accgatcacg ggcagcagca
      301 tctcggtctt ggtcagcacc gccgccgccg ccaggacgcc cccgatggcg agagaacccg
      361 tatctcccat gaagatctcc gccgggtgcg agttgtacca gaggaaccca atgatcgccc
      421 cgaccatcgc cccgcagatg atcgccacat catactggcg ctccaggaac gctatggccg
      481 tatacgcaag caacgcgatg ccgcccgccc ccgccgccag cccgtcgaga ccgtccgtaa
      541 ggttcaccgc gttcgtggtg ccagcgatga ccagcaacag gaagatgctg aacagtgcga
      601 cccccaggaa ccccggaccc agcacgaggt tgcgcttgat gcccggcacg atgacgttct
      661 gcgtgacgcc gacgtacctg agcgccagca catcagccac aaccactgtc agagtgagca
      721 gcaagaactt gtaacgcgcg ctcaacccct cgctgctacg gcgcgatatc ttctgccagt
      781 cgtcatacat cccgacaacg gccaccgatc ccacgagaag caatgtggtg aaagtcgcca
      841 cgttcggacg cgccaccacc accagcgccg ccaggagtcc catcagcata accaccccgc
      901 ccatggtcgg tgtcccggcc ttgatcaggt gcgtctgcgg cccctcctcc cggacgtact
      961 gaccgaactt ccgggtctgc aagaattcta tgaacttcga gccgagcgct accgtcacta
     1021 taaacgccac ggcggcgccg agaataacgc taaacaagag ctaaactctc cctcaacttg
     1081 cgggttaagg catcgagtct aacacccctg gaacccttga cgacaacgta gtctcgtggt
     1141 cgaagcgctt ctctcaggcc ctccgcggcg gattcggcgt ccgggaacat gagggtctcg
     1201 ccggagaacg tctcggcata ccagcgggcc tcgtccccga cgcagaccag gaggtccacg
     1261 cgcagttcct cggcgagctc gcctatctcg tggtggtagg ctctcgcgcc ggccccaagt
     1321 tcgaacatgc ctccaaggac ggcgaccagc cgccgccctt gctcgcgcga gccctcttcg
     1381 ccgtagcgca ggaccgcggc ggtcgccacc ggggaggcgt tataagagtc atcatagacg
     1441 atgatgtcat ctcgaagacg gaagacttct ccgcggagcc cggtgcgccg caccctcgat
     1501 acaccccgcg cgcactcatc cagggagagc cccagcgcca gcgcacctcc gatagccgcc
     1561 agcagcggct ctaccagatg agtcccgaag accggacagc gcacttccgc ctcctcatcc
     1621 cgcaggtgaa ccacgaagcg cagcccctca tcgcgctcct ccaagccgga gatccagagg
     1681 tccaccccct cggcgtggtc ccgggagaac cggatgcgtc gggcgaggtt gcgtcggtat
     1741 ccggtcgcgg cgtcgggagc gccatggggc gagacaaagg acccgctctc cggcaaggcc
     1801 agcgcgagct ctcccttggc ctcggccact ccctccaggc ttccgaacga gtcgaggtga
     1861 accggcgcga tggcggtaag tacgcccacc tgcgggggag ctatgctgca gaggtgttcg
     1921 atatcgccac ggtgggtggc gcccatttct agcaccagga tcccggtccg ctcgttggcg
     1981 gcgagtatgg tgaggggcag ccctatctcg ttgttgaagt tgccggctgt ggcggagact
     2041 ctcctgcccg cgacgcgcag gatagccgcg agcgcgtcct tggtcgtcgt ctttcccacg
     2101 ctccccgtta ttccaacgac caccggaggg tcttcgcggc gcaggctcca gcgggcaagc
     2161 tgctgcaacg cggcgagggc atcctcgacc acgagggtcg gaacatccag tgcgcggtcc
     2221 gcgacagtcg ccaccgcccc gcgaagatga gcctccgagg cgaagtctgc gccgtcggta
     2281 gcgccgcgca gcgcgaagaa aaggtcgccg tcccgtacga tccgggagtc cgtcgcggct
     2341 ccgtgggcga gcgtctccgg gtcgcatccc ttgaatgtag cgccgagtac ctcggcaatc
     2401 tgaccaagcg acgcagctct cattcgctcc ccgattcctc gggcgcgtca gggacgacat
     2461 tgaaatatcc gagggtaaag ctcatgactt tctcgaaggc cggagcggcc acgcgctcgc
     2521 cccagatcaa accctgcggc tcatccacgg ctattagcgt gacgtactct ggacgagatg
     2581 ccggcgcgaa acccacgaaa gatgagacat actcctcgcc ataggtgccc gtgacggggt
     2641 ccactttctg ggaggtgccg gtcttgccgg ccaccgtgta accgggtatc tgagcgaagc
     2701 ggcccgtgcc actgtctacc acgctttgaa gcatcccgcg tactatatcc gaggtctttg
     2761 cgcttatcac cctgggtccc ggtcccttcg gagccgcgtc ttgcgccacg tacggcgtca
     2821 ccttgagccc gccatttgca agtgcggcgt acccggcggc aagctggagc ggcgttaccg
     2881 tgagcccctg ccccatgggg atgttgccga tagaagaccc gctccagtct tcgtaggcgg
     2941 ggaccgaacc ggggtcctcg ccccacaggt ccacgcccgt tctatctcca aacccgaagc
     3001 gccggatgta ctgatatagg agctgcccgc cgagcccctc ggcgatctgg atggtcccga
     3061 cgttgctgga gcgctgaagg atgttctccg gatacatcac ctcggtcggg tgaggctcgg
     3121 agtcatggac caccctgtcg gcgacctgga tctggtccgc gaccgtaaac gtatcgtccg
     3181 gcgtcagcgt gccctcttcg agagccgagg cgatggtgaa cggcttgaac gtggatcccg
     3241 gctcgtaggg atcggtcagc acgcggtcgc gctgcacgtc ggcagacgcc tcggtataga
     3301 ggttgttgtc gtagcccggt tcgttggcga cggccacgac cgctccgtcg tccacacgca
     3361 tgacgacgcc cagcgcgctc ttcgcgtggt actccttcac ggcagataca agcgcggtct
     3421 ggagctcctc ttgcacggct gtgtctatag taaccctgac gtcctgaccg gacttcagcc
     3481 tcttgtcgta gcgcgcctcg accccgccga acgccttccc atggtcgcta agataaccgg
     3541 tgagttgtcc ggcaagctct ccatcaggat agacgcgatc cgaccccggc acgagagtta
     3601 tgccctctat tcccaggttt ctgacctttt cagcagcttc cggtccgaca gtccccaata
     3661 cactataacc ggacaaatgt cctcttgaat ctttctgcgt gagcttgctc tcgatctcgc
     3721 gctcgtcttg cccggtctcc gatttgagga tctgagcgag gtctccggct gcgtttttag
     3781 gggcttctat ctggtacggc gtggctacga tagtcgaggc ctccaggctt accgccagct
     3841 tgcggccgtc ggcgctcact atgctgccgc gcccttgcgg ggaaacctgc ctctgctggg
     3901 cgtcctgttc cgcggcgaaa gcctggtacc gctcgccatc agcgaagctc agatgcatgg
     3961 cgcgtccggt caggagcaga cttgctaccg taacggcggc tacgacggct cgcaatctcg
     4021 cgcgcccgac gagcggcgca tcctgggaga gctcagggga gaccgtccgg gtacgatgac
     4081 ggcgagcgcg cctgtgcgtc gggaaatcga cgaccttcgc ttcacgctgt tgccgttgcg
     4141 cggctcggcg ggcagcgcta cgggttttcc tcccgctgct tctctcccgc atatgcttta
     4201 ccatcctccc cgtttccgcc atccactctc atatcctcgc cgcccggctc gctcaggccg
     4261 aggtctctcg ctttctcgcg tatccggccc ggggccgaga gttccgccac ctgaacattg
     4321 agcttctgac cgtccgcttc cgcacgggct acccgctctt ccaggcccgc gacccgctcg
     4381 ccgagacttc ccgaaacggt gtgcaggtag atgctcccca gcatcagcag caccgggata
     4441 acgacaagcg ccgcgaaccc tctacggcgt tcgatctcct tgcgccggac ctttgcctga
     4501 tgggcccggt ctacgtggag ccgctcgcgc tctctaatcg gcgggacgaa gacgccggac
     4561 tcataaatcc ggtctctcct cccgggccgg ggaacagcca tcaatctcta tccccgtggt
     4621 cgttgagcgg ctcggcggtg cggatagcga cccggaggcg cgccgaggcg ctgcgaggat
     4681 tagcctcgac ctcctcttgc gtcggcttga tcgcggctcc cctccggaaa atcggcctgg
     4741 ctccacacac gcacaccgga aggtccggcg ggcatacaca gcgcccttcc cgctccgaga
     4801 taaagcgctt tacgatgcga tcctcaccgg agtgaaacga gatcaccacc agccggccac
     4861 cggctaccag cagacgctcc gcagccctca gcgcccgttc gagtcctcga acctcgtcgt
     4921 tgacgtggac gcgcacggcc tggaacacgc gcttcgcggg attcccccca cgctccttcc
     4981 aacccaccgc agcccggacg gcatccgcaa ggtctttcgt cgttcgcagc ggccggtggc
     5041 gcaagacctc tcgcgctatc ctgcgcgcct ccggacgagg tacgtcgccg tagttgatca
     5101 agatccgcac caactccgac tccgttaccg tatcaagaaa ctccgccgcc gaaacgcccg
     5161 agccgtgatc catcctcata tccagcggtc cctcgttaac gtaactaaag ccacgtccag
     5221 cggcatctat ctgaaagctc gacagaccga gatcgaagag gaccgcatcc acccacgccc
     5281 cttcctcgac cagcgtccgc agcacgtcgc caaattcgcc ggctctaaag ctaaagcgcc
     5341 ggtcccgaag gagatccgac gcccgtcccg ctgcttccgg gtcgcgatct atcccgatga
     5401 ccctcccatc gggagccagc ttctcgagga tgcgacgcga atgaccgccc ccgccaaacg
     5461 tggcgtccac gaccacgtcg ccatccgaca cagcgagtgc cgccaccgcc tcttccagca
     5521 tcaccggcct gtgacccgcc accatcatcc tccgaactcc cgccccgcaa actcctcgac
     5581 gatctccgcg aacgactcat ccgctccggt cacgtaacga tcccattctt ccgtatccca
     5641 gatctccaac ctgtcgtcca ctccggcgat cgtcacgtcc cgcctcaacc ccgcgtactg
     5701 accgagcttc gtcggtataa gaacccgccc ctggcgatca agcgtggcct cgaacgccat
     5761 cgagaagaac atcctggaga gctgccgccc cttcgccgtg agatccgcgt tagccttgat
     5821 attggccttg aaagccgccc actcgtcagg ggggaaagcg aagagacacc tgtccacccc
     5881 cttcgtaacc acgatcccac cctcaaagta ggaccggagc cttgaaggga gagtcagacg
     5941 ccctttctca tccagggtgt gctcgtactc gcctaaaaga gccagggtct ccgcctccca
     6001 ctatgctcca ctttgaccca caactgcgga aattgttaca taagcaggga atatacgcaa
     6061 cgcagcgccg taaatccgcc ctcagtacgt ccatctagag cggttttctg gctttatccc
     6121 tacccccaag gggtaagagt ccctacaaaa tcagagttga ctgccgtttt ggtgactgga
     6181 ggatctggag cgcacttggt cgaattgaag gcttatatag atggaatggt gtgcgaaacg
     6241 caatatgtag tgttttgttg aaaagttttg aggtgggacg aatttgaggc ggtgttcgat
     6301 tgcggtggga gtacgacccc gtttcggcgg gagggtgtat ggagcggccg ggggtgggcc
     6361 caccaggact cgaacctggg accgtccgat tatgagtcgg atgctctaac caactgagct
     6421 atgggccccc agtggctacg ggccgagacc tggggcctgg cctgctccac cgtgctcccc
     6481 tggtaggact cgaacctaca acccttcggt taacagccga atgctctgcc gattgagcta
     6541 caggggaaag ctgcgggtgc gattatatgc gtcgggcgag cgagcttcaa ggttcggagg
     6601 atacggctcg cagggcttcc ttgagcctct gtatctctga gtggagtcgc tccttggcat
     6661 cgaggtccgg cgcgtcctgc ttggcgcgct ggcggctcag aatggcgagc ctcagccacg
     6721 actccctgac aacggcttcg gagggataga gcttctcgcc ttccgcgtat agggctgcta
     6781 tctgctccat caagtctctg gctcgatcgt cggccaggac tgcgtcgagg tcatctcctg
     6841 cgtgctcgga taatagagtg aagagtcgag cctgggcctc gtccgcgaag tcaggcgcgc
     6901 ctagcatgac gggttccggt atctcggcgg ctgaaacccc ctcgcggagt ggtcgactgg
     6961 tgagatccgg ccgggtgagg atcagcgcga ggacttcgcg tccggcccgc agtatggggc
     7021 tctgggcggc gctctgtacg gttgagggcc gtgtcctcgc gtcccgacga ccgtctggac
     7081 cgtttgaccc ttgcagagcc tccgggctga ccccgagagc ctccgtggct acccggatcg
     7141 catcccgata gaatacggga tcgtcgatgt ctcttagcag gtcccgtatc tcggagaccg
     7201 tgcgagcgcg ttcggcggca tcagaacctc gggcctggtc ggccttgcgg cgaaatatgt
     7261 actccagaac ggacgtggct gcggtgagta agtcggtaaa ctcttccggc tggtgcttaa
     7321 ggagccagtc cgctggatct tcggagagac gcacgacgcg gagatccagt ttcaactcgg
     7381 aggccgtgac ggcggcgcgc tctatggcct tctcccccgc cgtgtcggga tcgaagagca
     7441 ggtagatcgt gtccgcgtag ccgctcaggg tcttgagatg agcgggggtg gtggcggtgc
     7501 ccagcgtcgc gacggcgttc tttatcccgg attggtacag catgagcaca tccgtatagc
     7561 cctcgacgac gagcgcggcc ctctcccggc ggatcgcctc cgcgacctgc gggaacccat
     7621 agagcagctc gcgcttgttg aatacgtcgg tctccggcga gttgaggtac ttggggtgat
     7681 cgtctccgag cgcgcgggca ccgaacccga ccatcctccc ccgccggtca gagatgggaa
     7741 aagtgatcct gccggcgaac cgctcgccgc cgcgggagga gagaatcccg gcggcgtcca
     7801 ggatgcgacg gtccagttca aaccttccgg ccacccccga aaatcccgaa ctgcctctcg
     7861 gcggcgcgta gcccaaccga aattcttcta tagtagaaag taccaatccg cgctgtttca
     7921 ggtactcgcg agactcttcg gcctggggag tttgggactt cagcaggtac ttgtgatagt
     7981 agatcgcggc ggctgccagc gccttgtgta ttttgcgtcg gcgaagggtg cgatcccggg
     8041 cggctttcga gtcggcttca gagcctccct cgaactccag ttccgtcccg gagcgttccg
     8101 ccaggtacga gacggcctcg gcgaaggaga gggatttcag gtccgtgacg agtttgatgg
     8161 cgtcgccgcc ccgctggcaa ccgaagcagt agtaaaaacc ctgctctgga gtaacgctaa
     8221 aagaaggcgt cttctcctga tggtcgggat agggacagag accggagaag cgggcgccct
     8281 gtcttctgag cgcggtgaac tccgaggcga cctcgacgat attagaggtc tcacggatag
     8341 tctcgatgct gcgctgcgag atcctcatct acgctcgttt gttatacgtt cgtacaaaaa
     8401 tattccctga tcctagatcg aatgcgcgct aatgttactc tacaaaccat gaatatctcc
     8461 cgctacctgg atcagaaatc cgacaacgag tacgcgaacc tgccgccggg cgtagagttc
     8521 gagaagaaag acgagcctct ccgacgggat ataaacctgc tcggccgcat cctgggacag
     8581 gtgttggtcg agcaggaggg cgcggatctg ctggatgcag aggaggacct gcggctcctg
     8641 tgcaagcggc tgcgcttcga ctacgacccg gagctcgacg agcgtctgag acggcgcata
     8701 gagaagatgg acgcgggcga actgggacgg atcgtgcggg cgttctccgt gtattttcag
     8761 cttgtgaaca tcgcagagcg ttaccatcgg gtacggagac ggcggcaata cgagacctcg
     8821 cccgacaacc cgccccagag ggcgtcgcta gccagcgcgc tctcacggct cgccgcagac
     8881 ggcatggacg ccgctcaaat tcaggagacg attgacgacc tgaacgtggg gctggtgctg
     8941 acggcccacc cgacagaggc tctgcgaagg agtgtccggc gcaagcacga gaggatcggg
     9001 gagatgctgg agcagatcga atccggtctc actacctgga aggagcgcca gcgtcttgag
     9061 gagaagctgg cggaggagac aacgatcctg tggcagacgg aagagttgcg ctcgcggcgc
     9121 ccggccgtct cgcaggagat acagcgcacc ctgctcttct tcgagcaccc gttgatagac
     9181 gccaccctag acgtctaccg ggagttcgag aacgagctgg cgaaccgctt cccggattct
     9241 gccttggagg tcgggagggt actcgagttc ggctcctggg tgggggggga tcaggacggc
     9301 aatccgtttg tgaagccgga gaccctcacc acagccctcg gcctgcaccg ggaggtgatc
     9361 ctcaagcgcc acatatcggc ggtcctcgca ctggcggatc acatgagcca gtcctccaaa
     9421 ctggtcgagg tctctgagga gctggcggct tcagtggagc gggcgtccgt gtctcagccg
     9481 gctgatgtgg agcgccaccg cggcgaggac acgaacgaga tctactaccg caagctcacg
     9541 atcatcgccg agcggttgag gctgaccctg gactcccccg gctcgcgcga tgcctactcg
     9601 ggcgtctcgg agttcctgga cgacttgatg atgatccgcc gcagcctcat cggtcgcgga
     9661 ggagaacgag tcgccgatgg aggtttgaga gacctcatcc ggcaggctga ggtattcggg
     9721 tttcacctgg cgaagctgga cgtccggcaa gagagcagca gggtgatgga ggcagcaggg
     9781 gcgctaatct cgccacatga aggcaaggat ctcgccgaca tggacgaaga ggagcgcgca
     9841 gctctcctgc gggacctggc cggagacccg gacctggacc ttcctcagcc ggacggagtg
     9901 acggaggagg tccgggacgt actggagact ttcgagcgta tccggcgcgc gaaggacgag
     9961 ttctcggagc cgccggtcga aactttcatc ctgagcatgg cgcggcaggc gagcgatgtg
    10021 ttgagcgtgc agttgctggc gaggcgagtt ggcctgttgg aggtagacag cgagggccgc
    10081 tgcgaggcga gttatctgcg gataacgccg ctcttcgaga ccatcgagga cctggagcgc
    10141 gcgcccgagg tcctgcggcg gctcctcgat gactcgttct accgctcgtc tctcgcaaag
    10201 agcggcgacg tgcaggagat catgctcggc tacagtgact ccgggaagga cgcgggctac
    10261 gtgacgagta actggacgct gtacaagacg cagagccggc tgaccgacgt ggcgcacgag
    10321 tacggcgtaa agctgcgact cttccacgga cgcggcggca gcgccggacg gggcggcggc
    10381 cccagctaca acgccatcat ggcccaaccc cccggaaccc tggacggcag catcaggatc
    10441 acggagcagg gcgaggtcat ctcgttcaag tacagcatgc ggggcctcgc ccggcgtaac
    10501 ctggacacag tcctcgcggc cgtgctggaa gcgaccgtga cgcagagtca cgaagacctc
    10561 gcccctgaat ggacatcggt cctcgacgaa atctccgggc gagcgtccga gacataccgc
    10621 gccctggtct acgaggatga ggactttcta ccgttcttct ccggggcgac cccgatcgag
    10681 gagatctctc ttctgaacat gggcagccgc ccggcccgcc gcgtagggaa gccggacgtc
    10741 tcaagcctgc gggcgatacc gtgggtcttc gcctggaccc agaaccgttt cctgctcccg
    10801 tcctggtacg gcgcgggcac ggcgctcggt aactacatcc agcaacctga cggcctggcg
    10861 accctgcgag acatgtaccg gcggtggccg ttcttccgca ccctgacgga cttcatgcag
    10921 atgaccctgg cgaagagcga cctgcgaatc gcgcggactt acaccagcct tgtggaagat
    10981 gcaggggtgc gggaacgcct gtggcagcgt atcgccgagg agcatgcagc gtgctccgag
    11041 gccatcctgc tcatcactga gaacgacaac ctcctcgatg acagcccggt gctgcaacgt
    11101 tcgatcaggc tccgcaatcc ttatgtggac ccgctgtctt atattcaggt cagcctgttg
    11161 cgccgcctcc gaagccttcc cgaagactct ccggagcggg cgggcgtgct cgaagctctg
    11221 cttctgacgg tatcaggtat atcctccggc ttgctgaaca ccggctagcg tctacgcgaa
    11281 gatctcgcct cgcggcaaaa agagcctgcg gtaagtcgcg atagcatagc ggtcggtcat
    11341 accagccacg aagtcaatcg tctctgaaat agggtcctcg tcgctgttgg cgcgctcctc
    11401 ggcgtgctcc aggtaatact cgaagagcga agatacgacc cccgccgcct tgcggttctc
    11461 tctggagtcg ggattgcggt acacgttatc gaagagccac gtccgcaact ccatgagcgc
    11521 aacgtgaacc ctatcggaga gagctatctc accctgtttc tcagatgtag aaatcatgtc
    11581 gcgcaccagc gtgtctatcc tggcgctcat ctccctgccg agtatggcca gaggcgcctg
    11641 tggaaggtct tcaaagtcta ttacgcccgc tcttagggcg tcatccacgt cgtggttcac
    11701 gtaggcgatc ctatccgcga tccggacgat ctcgccttcc agcgtcgcgg gatgaccttt
    11761 ccctgtatgg ttaagtatcc catctcttac ctccagggtg aggttgagtc ctttaccacc
    11821 tttctccagt ttgtccacca cgcgcaacga gtgctcattg tggcggaagc tgcggccatg
    11881 gggagcgaga gcgcgggcga gagcctcctc cccggtgtgt ccgaacggag tgtggccgag
    11941 gtcatggccg aaggccacgg cctccgcgag gtcttcgttc agttcgaggg cgcgggcgat
    12001 ggtgcgtgag atctgcatca tctccagcgt atgggtgagg cgggtacggt aatggtctcc
    12061 atctggagcg atgaagacct gcgtcttgtg catcaggcgc cggaaggcct ttgcgtgaat
    12121 tatgcgatct cggtcccgct gaaactcgtt gcggacgccg tcgggtggtt ctggcttgac
    12181 gcgaccggcg ctcttctcac acgcccgaga gcgtagagtt gtcacgcggg tcgccgggtc
    12241 ccggtgtcgg acaacggcgc ggcggtattg accgtcgcac caggagcgtg cgcggaaagg
    12301 tgcgcgcgga ctactcgaag gacgctctct tccagaccat cctggaccat ggcgtcccgg
    12361 acgggacgct cgatcaaggc atggagactc tccagcacct gcggcgccag gggaaaagtg
    12421 tcgctcgccg cagcccggca ctccgcgcac aggacgccgc ccagacccgg tgcgaagtac
    12481 ggcggtccgg atacttcggg atcggcgccg cagttcaggc aggcgtcgag gtgcggagcg
    12541 tacccggcca ggatcgtgag cttgaggacg aacgcggctt cgaccgcccg atagccagag
    12601 tcgcggcttt cgagcgaatc gagagcgtgg taaaggaggt tgaagacccg gcggtcggcc
    12661 tcatccccac cagagagggc gcgcacggcc tgaaccatcc tgcccgccgc ctcgaaccgc
    12721 gccaggtcct cgcggactac gtggaagctc cgcagcacct cgacctgggt gacggtatcg
    12781 agcgtgcggc ccccgtaggc cataaagtcc acgcaagata gcggctccag ccgcgctcca
    12841 aaccgggagc gagtgcgccg gatgcccttg gctatcgccg agatcagacc ggcgtcctgg
    12901 gtatacaggt caaggatgcg gtcggcctcg ccgtaacgga tcgagcgcaa cacgatccct
    12961 ttactcctgt agagcgccac cctcggcctc ttccataatc gccacgctgg cgctcatacc
    13021 gaggcgcgac gccccagcgt tgagtatgtc gagcgcctcg tccagggtgc gaatgcctcc
    13081 cgatgccttt acggccagtc cctccggcgc ttcttcgcgc aacaacgcca catcctctac
    13141 ggtcgcgccc gacggagcga agccggtaga ggtcttaata aaatccgcgc cgctggcggc
    13201 gacgatctgg gtggccagcc gcttcatctt gtccgtgagg tacgcagtct caactatcac
    13261 tttaactaca acgtcgttga gcccattgtt catcgcgaca acgctgactt cctggttgag
    13321 gctgacgagc tcctcggcga catagttgaa gtcgcccgag aggaaccggg agatattcat
    13381 gaccacgtcc agctccgagg ccccgccgac gatcgcgtcc cggacagcct ggaccttgat
    13441 agaggtggtg tccgctccga aggggaagga gactacggta gcgactttca cgtccacgcc
    13501 gcgcagctgc ttggccgcaa aacgtacgta gcacggcgga atgcacacgg cggcgaagtg
    13561 gtagtgggcc gcctcttcac acaggcgagc gatgtcttgc tccgtagcat ccggtttgag
    13621 gagcgtgtga tccaccgtct tcgcaaactc ccggaccctc atagccacgg ctcgacctcc
    13681 cgactccact ctacccaaca cacccccgac attataaccc cagcctctcc aggaacttgg
    13741 gatcactcct ccatcctgga gtaaccttta ccttcaggtc gagatagatc cgggttccga
    13801 gcagacgttc gacgtcgcgg cgggcttcag tgccgatctg cttgatggtc ttgccgccct
    13861 tgccgaggac tatcatacgt tgggtggcgc gctcgacgtg gatgacggcg tagacaacgg
    13921 tgacgttctc cttacgctcc acctcgtcta tctcgacggc gacggcgtgc ggcacctcct
    13981 cccggagcac gctcaaagcc ttctcccgga cgtactctcc gaggatcagg gtctccgggt
    14041 aatctgttac ggtcccctca gggaagtatt gggggccaag aggcagaagc cgggagaccg
    14101 tttccataag cggctcgacg ttccgcccgg tgacggcgct gagtagaaag acgtccttcc
    14161 atccggcgag cttcgagacc tcatccacca ggggaagcgc atcggtctcg cgcgagagca
    14221 ggtccacctt gttgatcccc acgaggaccg gagtgccgga gcgagcgacg aggtccgcaa
    14281 cgtagcggtc gccgctgccg atgcccctcg cggcctgcgc ggcgtccagt aggaagagga
    14341 tcacgtccga ctcgcccaga ctgtcgagga cctgttgctg catccgggcg cgcagggtat
    14401 cgcgcggttt ctgggaaccg ggggtgtcga tgaagaccgc ctgatagccg ggaccattac
    14461 gaacgccccg caccggcccc cgcgtcgtct gaggacgcgg agaggtgatc gagaccttct
    14521 ttcccaccag ccggtttacc agcgtggact tcccgacgtt tgggcggccc acgacagcga
    14581 cgaagccgct ccgcaggtct tcctcggcga gcgcgtggtc cagcgcctga ctcatagcga
    14641 ctccggcccg aaggagttgg gcagtatctc ttcgaaggga tagctgcgaa gaccatccgc
    14701 gtcttcgacc accacctcct tgcagccaaa ctcccggagc acctgccggc aggcgccgca
    14761 ggggaagcac ggcgcggcgt tagggctgtg gacggcgacg agccggacgc tcctgtcgtc
    14821 cgggttcgag agcgccatcg cggctgcggc gttgcgctcg gcgcagatgc cgagcccgta
    14881 agaggcgttc tcgacgttac agccggggaa gagctttcca ccctcggtca ggatagcggc
    14941 accgacgtgg aagttgctgt agggcgcgta ggcgttgcag gcggcggaac gagccgcggc
    15001 catcacgtct tggcggtcca tacttacctc cttcaaaagc ggcgtcatag gaactgaaag
    15061 atcgcgactg ttatgaggac cccgatcagg gccccggcca cgacctgata gacgtcgtgg
    15121 gtgccgctct ccacgcggct ctggcacacc aacagggcca tgagtaaggt tatcagggac
    15181 acgagacccc cgtagcgacc ctcggccgct atgaagctgg cagccaccca gcccgcgaaa
    15241 gacaccgccg cgtgcccgga cggcatcccg ccggagaacg agttgggcgc ccgcgtgaga
    15301 gccttgatga gcagcaccgc aagagtcacg acgcccagag agaccagcgt gaggtgtccc
    15361 ggccaggcgc gcaccgtctg cagggcgttc agggagagcg gcccaaggtt gtcggccaga
    15421 atcagatagc ctaccagcac cgcgccgata ctcgtaacca acacggctcc ggcggagacg
    15481 tccttggcga gtttggcgag aggatggtat tcgcgcgttg cgaggtccac cgtgaactcg
    15541 atggcggtat tgaacatctc ggtgaccagc acgaccagga tgacgatcac gagcgccgcc
    15601 aactcgagcg cgctcacctg aaccagcagg ctaagtacca gcactaacac cgcggccacg
    15661 acgtggaagc gcatgttccg ctgcgtccgg accgcgaaga tgagaccacg atatgcgtgg
    15721 ttgaagctgc gggcgacgcc ctgctggccc ttctttggct gctccgccgg tcgcggctcc
    15781 tctagccgcc tatcctgctc gctcacgcgc gctctccatt acggccgcct gcacgcgggc
    15841 catctcggtg tcctcgaact gatctccgtg gtccatgccg ctcaggtgca gggctccgtg
    15901 aaccacgagc tcctccacgc ccatctccgg gtgcgcgcag tccggggcga tgacgacctc
    15961 tccgaccatc tctccatatg gcccgtccac ctcgaagctg aggacgtccg tcgtgcggtc
    16021 caccccgcga tactcgcggt tcaactcatg gatcgtctcc gggtctacga gtgcgacgga
    16081 gacctcgccc agccgctcca ggtcgaagcc ccgggcggag aaggcggtgg cgcacaggtc
    16141 cgtgacgcgg gcggcatcca gtcctccgta gctgacctcg tccagtaacg ctaccgagaa
    16201 aggcacggga gtaccgcggt tcaggtctgg ctcccggcgg gcttgcccgc gatctgaccg
    16261 acaggcggct tcttctcggc gggggtagct tccgggtact cgatgcgggg tcccaggaag
    16321 ccgatcaggg cctccagtat cgcctcgccc acggcgtgta tctcctgcat cgtcagctcg
    16381 cactcgtcga actgaccgtc ttccagcttg tgccggatcg tgtcggagac cacgtcctct
    16441 atgcgcttag gcgtcggctt ggcgatggac ttcacggtcg cctctacgga gtccgcgatc
    16501 atgacgattc cggcctcctt gctgcgcgga cgtccgcccg agtaccggaa ctctgactcg
    16561 cgcacggtcg tggggtcggg ggcgaggtcg cgcgccttgc ggaagaagta ttctatgcgc
    16621 gtggtgccgt ggtgctgagc gattatgtcc aggatctcct gcggcagccc ccacatgcgc
    16681 ccgatctcca gtccgtcctt gacgtggcgc tttatgaccc tggcggagag agccgggctc
    16741 agagtcgcat gcgggttcac ctgagcgatc tggttctcga tgaagtacgc tggatgctcc
    16801 atcttgccga cgtcgtgata gtaagccccg acacgcgcga gcagagtatc cgccccgatt
    16861 cgctccgcgg cgttctccgc caggtttccc acctgcatcg agtgcgagaa cgtgccgggc
    16921 gtcgtccgca ggagcttctg caacagctcg ttgccggggt cagagagctc cagcagcttc
    16981 attggagtga gtatgttgaa agcgtcctcc agcagcggca gcagcatcat ggcgatcatg
    17041 agcgagagca gaccatttcc cagaccaaga gcgccctgct gcagcgccac gcccaggtcg
    17101 ctgccgccca ccaaactgac cgcgaatgta ataccggcca tcacgacggc gatgaacaga
    17161 ccggccttca gaagctgttg acgcgaggtt acgcggacta ccgtatagat ggcgaagccc
    17221 gatgtcagga gcagcgcggc ggagagcagg aagtcattac cgccaattat gccgacgttt
    17281 accgccgaga tgacgaccat caggaacatc aggcgcgggc cgaggaggat agtgccgatg
    17341 atcgagagcc cggccagcgg tatcaggtag gggtcgagcg agagcaggac gaaccctcgc
    17401 gcgagcaggg tgaagaggat cgtaagggag gcggccagga cgatgcggat catggcgttg
    17461 ttacgcagga tgcgttttcc gaaccgctcc aggaagtacc acgcgacgcc catctcggcg
    17521 gccacgacga gcgcgacacc cagtataacc gtccacgggt tcgtatcgcg cacgatgctc
    17581 aaggcctgca actgcgctat cgcctgacgg tctataacct cgccgcgcgc gaggattcgc
    17641 tctccctgtt tgatgctgcc ctttaccggc ccgaccttcg aagcagcctc gtcacgggcc
    17701 ttctgggtgg cggcgcggtc aaccacgtaa ttcggctcca caaactcgcg gctcagtacc
    17761 tccaccagag cgccctcctc cccagaggca tcccgcacag cggcctccga gagccggttg
    17821 cgagcctcgg agatcctcac cacggaggcc ggaacctctt cgaccgtatc gtcagccacg
    17881 gcggtagagg cgtacatctc ctggaggttc tcggtgacgt aagtctcgat gttttcgagc
    17941 tccttgtcat cgacgaatac cagagcccgc gccgacgtct gaggcagata cgacggcgag
    18001 gcgtcggaga tctcctgcgt cttctgctcc gcgggtttgt cggaggcccg gatcttcctt
    18061 accttgtcga agaattgctt gacgttgccc tctacgtccg ccgggacctt ggagctctcg
    18121 cggtagacca cggcgaccgc gtcggcggcc tcctggcggg cttcctcggt ggcggtaggg
    18181 tcggtgtagg tgacgctccg cggcgcgtac acgtcggcgt cggagacggc cccaacctgg
    18241 tagtcctgac cgttatcgct gaaccccaac gcggtaagcg gccggtcgtc gagacctatg
    18301 agcccggtaa gggagatcca tgtcacggcc gtgatcgcca cgtagagcct catacggggc
    18361 aaccgctcga cccacgccct gaatcgctcg aaccgcgtcg gtgggcggtg gaattcatcc
    18421 gtcctgttgc cgttgagcgc ggcccttttc tctttgagtt tcacgacatc tcccacgcgg
    18481 gcacttttta ggccccgcta cctccctggg catcctcgta ggcgtccact atacgcatta
    18541 cgaggtcgct ccgtacgata tcgtcgcgct tgagcccaac gaacttcacc ccctcgacac
    18601 ccgtgagcgt ctgacgggcg tcttcgagac cactgcggcg gccatgcggg aggtctacct
    18661 gcgtgacatc gcccgtgacc accatcttcg acccgaaacc gaggcgggtc aggaacatct
    18721 tcatctgctg gggggtagta ttctgcgcct catccagaat gatgaacgcg tcgttcaaag
    18781 tccggcctct cataaacgcc aggggtgcta tctcgattat accgcgctca aggtagccct
    18841 gaaacttgcc cggatctatc atctcgtaga gcgcgtcgta gagcggccgg aagtacgggt
    18901 ccaccttcgc catcatgtct cccggcagga accctagaga ctcccccgcc tcgacggcag
    18961 gacgggtcag tatgatccgt gacacctcgc cccggttgag agcgtccacc gccagagcga
    19021 ccgcgagata ggtcttcccc gtccccgccg gcccgatgcc aaacaccacg ggcttctccc
    19081 ttatggcgtc ggtatatgct ttctggttgc gcgtcttggg ggccacgcgg cgtccgcggt
    19141 gagtgaggat aacgtcgcca agcacccggc gcccttcgcc gccgtcgccc atcctgtaga
    19201 gccgctcggc cgtctccggc gtcgggtgat ggccgtcctc ggcaagcccg acgaggccgt
    19261 tgaataccgt ctccgcccgc tcgacggcag cctcggtgcc gcgcagtacg atctcgttcc
    19321 cccgcacgat gacctcgcac tccaccaact cctcgacccg gcgcagcacc gtgtcccggt
    19381 cgccgaagac ctccagcata cgtcccggcg gtatgacgat cttggcctct accttgctac
    19441 ccgttgaagt atccgtgtgt cctcctccgg tgaaacttac cttaaccggc gcacctactg
    19501 caagcggcct ctaaccagcg ccgccagatg acgcccgtcg gcccttcccc cggcaagcgc
    19561 cgtcgcccgt cccatcactc ttcccatatc cttcatctcc gaagccccgg tctcatctat
    19621 cgcctgctcg acgatacgct ccaactcatc ctccgagagc ggcgccggca ggtactccct
    19681 gaccacctcg gcctcagcag cctccgaagc ggcctgctcc tcgcgcccag ccttgccgaa
    19741 agcctcggcg gactcctcgc gctgcttgag ctgacgccgc agtacggcct gctcctcctc
    19801 ttccgagagt gatctcccgg cctctatctc gccattcttt agtgccgcgt tcagcatcct
    19861 gagcgtctcc accctgcgcc ggtcgcggtt cttcatcgcg gctttcgtgt cctgctgtat
    19921 ctgatccgct atgctcatga attctatcct ctctccacct tgtggccgaa actttaatcc
    19981 tacaaggtac atgctcgcaa gcagtcagga cctggccctc ccgagatcag acctgtccag
    20041 caccgcccgc gcgcgcgcca cggcgatgat ccctgccgtc tcgctcctta acctgtaggg
    20101 tcctagctgc gctgccatca acccggcctc tgtcgcgatg cgcagctccc ccgcgctcca
    20161 tccgccctcc gggccgacga agaggctgac ttcgtcttcc cgcaaacagt tttcgagcgg
    20221 cggaaggtcg ggcgcattgt gcaatatcac cccggtcgca ccaactctgc ggacagcctc
    20281 ctcaaacatc acgggctccg gcacttcggg gatgcgaagc tgcagggact ggcgggccgc
    20341 ggattccgag atcctccgcc agcgctccaa cttcgcagcg cgctcgtcca accggaccac
    20401 tccatgctcc gttacgagcg gcacaacgcc agcggccccc aactccgtgg ctttctccac
    20461 gacgagatcc atatgccgcc ccttcgggac cgcctgataa agaattacct ctccacccgg
    20521 cgcgggttcg ccaccgagcg actccaccac gaccgcctcc cgtccctccc gaagctcggc
    20581 caggaataag cagcgcgcgc catcaacgac ctcgaaagca tctcccggcc tgccgcgcag
    20641 taccttgagt acgtgatgcg actcctcttc cggaaggttg aaggactcgc ccttgatcaa
    20701 ggtccagtcc acgaagacgc gcgtgatcgg cgtccgagag atccgggctg ttatcggaaa
    20761 acacctctta gccggtcgaa gaacgtcccg tgcgaaccgt tgtacgtctc ttccccgctc
    20821 accgcgccga acctctccaa gagctcgcgc tgctcggccg tgagccgggt cggtaccatt
    20881 acgtccacca cgaccttcag gtcgccgcgc ccgcgccccc gcagacgcgg catcccctcg
    20941 cccctcaact tcagcgtagt gcccggctgc gtacccggtt cgatctttac ctcggtccca
    21001 ccgttcaacg tcggaacctc gacctcagtg ccgagagccg cctccacgac gttgaccctc
    21061 atccggtgga tcaggtcttc cccatccctc atcaactccg ggtcttcctt gaccttcacc
    21121 acaacgtaca gatctcccgg cggagcgccc ggctcgccgg catgtccgcc gccggagatc
    21181 ctgagcttca tcccctcctc gatgccgggc gggacgcgca gcctgtgaga ctcgacctcg
    21241 gagacccggc cactcccccg gcagttgtcg cagtacacgt caataaccct gccgcggccg
    21301 tcgcacgccg aacatgcctg cgtggtgacc atctgaccca ggaggctctg ccggaccgtc
    21361 ctcaccgctc cggctccgcc gcacgtccca caaacgtgcg attctgtccc ccctactccg
    21421 tcacacacgg agcagttctt aacagtctga acttttacat cacggtcagc tccgaacgcc
    21481 gcctcgcgaa gatcaacctc gacttcaacc tgcgtatccg cgccgcgagc cgggccgcgg
    21541 gagccggtgg agccgaagaa gggatcgccg ccgaaacggt ctccgaagaa ggcctcgaag
    21601 atatcctgga agccgccaaa gggatcgccg ccgggatatg cgccgcctcc gcgcggcacc
    21661 tgatgaccgt aagtgtcgta ggcgcgccgc gattcggagt tggagaggac ttcgtaggct
    21721 tcggtcagct ccttgaaacg ctcctcggct ccgtggtcgt ccgggttggc gtcagggtga
    21781 tggtcgcggg cgaggcgccg gtaggctttc ttgatctcct gctcggaggc ttcgcgggga
    21841 aggcccagca cttcatagta gtcgcgcttc gtcgccaagc ctaaaacctg gtgttgaggc
    21901 agtgggtcag ggcgtcggcg gcagagcgca cagtggaaat ggccgcgtcg tagtccatac
    21961 gcttcggacc tatgaggctc acgacgccgt agggacgctc gcggtggttg taggcggcgg
    22021 ctatgaggct cgtgcctgtc aggttgtgga agccgttctc cgcgccgata gagaccacga
    22081 cgccgctgct ggatacagag cgggcaagcg cgtcgcccag tagattcagc agccaccggc
    22141 ggcgctcgaa gatctcgatg acggccgcga ggctcgccgg gtcgaggtcg tccagccgcg
    22201 ccagtagcgc cgacgcgccc tggataaaga ctccgcgctc cgtggcgcgt ccgaggaccg
    22261 caacggcctc aaggatcgcg ccgaccacca gcgggttgtg gtcggagaga gctttccggg
    22321 cggcgaggcc cagttccagg tcggtgccga cgggccttcc gcccagccac gagttgaggg
    22381 aagagaggat ctcgcgcatc tcctcctcgt ttacggagga gggcagcgag atcgtcgcgc
    22441 tggcagaggc tccgctctcc atcgagacga ccagcaggaa cgctccctcg cgcagcggta
    22501 gacagtccac ccgagccaga gaatcgccca gggcgctcgg tcccgcgacg acggccagca
    22561 ggcgcgtaac ctcgctcatc ccctccgaca cgccacgcag aagctcgtcc acgttaccgt
    22621 aaccgtccgg cgtctcgaac aacgtctctt ccggcggctt tccggccagc agggcgtcca
    22681 cgtacaggcg atagcccgcg tccgtgggga cccgtccggc ggaggtgtgc gggtgggtca
    22741 ggagaccgcg ggactccagg tccgcgagct cggcccggat ggtcgagctg ctggcgtcta
    22801 tctcctcgga gagcgtgtgg ctgcccaccg gggcgccggt cgagatgtac atctccacgg
    22861 tcttcgtgag gatgtcgcgc tgcctgtcgg gtatgtcgaa agctctcata ctgctccgaa
    22921 ttctaccatg caaggctccc ggcccaagag cctcgggccg ggatcagata cggagagcgg
    22981 ccaggaacgc ttcctgcgga atctccacgc gcccgacctg cttcatgcgg cgcttgccct
    23041 tcttctgctg ctcgaggagc ttcttcttcc ggctgatgtc gccgccgtag cacttcgcga
    23101 tcacgtcctt gcggtatgcg cggacggtct cgcgggcgat gatgcgcttg ccgacggatg
    23161 cctggaccgg cacctcgaac tgctggcgcg ggatcaactc cttcagcttc tcggtcaggg
    23221 cgcgtccgcg agcgtatgcc ttgtcgcggt gggcgatgat cgagagcgcg tccaccgggt
    23281 cgccggagac cagcacctcg actttcacca ggtccgacgc ctcgtagcca agcggctcgt
    23341 aatcgtagga ggcgtagccc ttcgtgcggc tcttcagggc gtcgaagaag tccgtgatga
    23401 tctccgcgag cggcaggtcg taggttagct gcacgcggcg cgtggagatg tactccatcc
    23461 cgatggagac gccgcgcttc tcgtggcaga gacccattac agccccgacg tactctttcg
    23521 gcgagatgat cgtcgcccgc accacgggct cccggatctc gtcgaagatc tccggcagat
    23581 cgctcgggtt tgtgacttcg atagtctcac cgtttaccac gacctcgtac ctcacgctgg
    23641 gtgaagaggc gatcaggtcg aggtcgaact cccgctccag acgttcctgg atgatctcca
    23701 tgtgcaacag ccccaggaaa ccgcacctga acccgaaacc catccgagag ttctccggct
    23761 cgaagaagag ggaagcatcg tttagttgca gcttggagag cgcgtcccgc agccgctcga
    23821 agtcgtctcc atccgtcgga tacagcccgg agaagacggt cggcagggcc tccgcgtatc
    23881 ccggaagcgg ctccgaggcc ggatgttcaa ccgtcgtcag cgtgtcgccc acgcgcagag
    23941 cctcgatgtc cttcaggccc gcgatgatat agccgacctc gccggtcccg agcaccggaa
    24001 gagcggtggg tttcggggag tagcagccga cttccagaag ctcgaactgc tcgtcgctgc
    24061 ccatcgcgcg gaccttgtcg cccttcttca actcgccgtc gaagagccgg gccagggata
    24121 ctacgccgcg gtagggatcg tagtaggagt cgaagatcaa ggcccgcgtc tcctcgcgct
    24181 ccgtagccgg cgcgggaacc cggtccacga tggcgtcgag gacatccgta acgccttcgc
    24241 cggtcttggc ggagatcttg agtatgtcct cctcgtccac tcccaacagc tcgacgatct
    24301 cctccgtaac ggcgggcacg tcggctatgg ggaggtctat cttgttgagt acggggatga
    24361 tctccaggtc gtgctccatc gccaggtaca ggttcgcgag cgtctgggcc tggatgccct
    24421 ggctcgcgtc cacgatgagc agcgctccct cgcaggcggc gatagcgcgc gagacctcgt
    24481 aggcgaaatc cacgtgccct ggggtgtcga tcaggttcag cgcccactcc ccgtcgtccc
    24541 gtttgtacag gacgcggacg gcctgggcct tgatcgtaat gccccgctcg cgctcgatgt
    24601 ccatggtgtc caagatctgg gccatgcggt cacgctcggg gacggcgtcc gttatatcca
    24661 gcagccggtc cgcgagtgtg ctcttgccgt gatcgatgtg cgcgatgatg cagaagttgc
    24721 gcgtacgttc gatgttcttc acccgcagag tctaacatga ttgccgcttt gttccccatg
    24781 tgttacattc ttcaggttga gtcgttgaca gtagcaagag gagcaagcag cgtgccagca
    24841 ccatccaagc gggacaagca gagcattaag cggggagcgc agaacaaggg tgtccgcaca
    24901 cacctgcgca acctctccaa gaacttctac aaggcgctcg aagccggaga cgaggataag
    24961 gcccgcgaga tccgggacca gtcccagagg gcctacagca aggctgccgg aaagggcatc
    25021 atccacgcca acaaggcgag ccgcaaggtc agccgcttcg acaaggctct cgccaccgac
    25081 cgcggctaga accagccact agatacgcac ttctccgccg cctccgggtg gcggttttct
    25141 tttgctcgtt ttttaccggg aaagcttcat cacggccatc tcgacctgaa gctcgtcggc
    25201 caggtcgcgc ccgcccttga gaccacgttc gaggtccagg acgatgccta gcgcgtcttc
    25261 gagggtccca ccatctaacc ggcggctctg atcttccagt ttcctggcta caaaaggcgg
    25321 cacctttagc tggctcgcga tctcgcttcg cggcgtgccg gcctccgaga gtgcgcgggc
    25381 gcgggccagc aggtggaact ggcgccgcat catgtacgtg acgcgcagcg ccggctcgcc
    25441 ggtcccgatg aggtcctcca gcaaactaat cgccctgccc cggtccccgc tacccaggct
    25501 atccacgaag gcgaagatgt tggactgcaa gtcagggaga catagcttat ccacgtcttc
    25561 gagcgtagca gcgccgtttt ctacatagag ggctagcttc tcagcttcac gggccgaccg
    25621 caccttgtcc ccggagcaac ggtccgtcag gtcctgagcg acgtcctcgg atatttccag
    25681 tccctgtttc ctggcgtagc cgactaccca cttcacgaga gccttgccgg ttggctgcgc
    25741 gaagtcgtgg acctcgccgg acttcttgac cgcggcgagc agcttctctc tcgcgccgag
    25801 cttcttaccg agcatgacga ggtcggtgtc gggagacggg tcctccacat aaccgaggac
    25861 cctgttcttc tgggccgcgt tccacgcatc gaggttgcgg agcagcacca tcgta
//
LOCUS       DASSMB010000266         5321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1238168, whole genome shotgun sequence.
ACCESSION   DASSMB010000266 DASSMB010000000
VERSION     DASSMB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5321
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238168"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1048
                     /locus_tag="VFJ72_15270"
     CDS             128..1048
                     /locus_tag="VFJ72_15270"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7480863.1"
                     /translation="MERREESLAALAALSDGMAALVEQTGPAIVRVHGRRRPSSGVVF
                     TPEMVLAASHALEREEDLSVGTHDGRTLGARLIGRDPATDLAVLKVEGLAVEAARLAE
                     GEARVGQLSLAVGRSGRGEGPIVSVGVISAVGGPLRMRWGMRLERYIRTDAQVFPGLS
                     GGALVDARGGVLGVLTTGLARGVSLAVPAELAWRVAGEISERGSIRRGYLGILSQPVR
                     LPDAQRSGLSQRDGLLVVGVEDGSPAASGGMMVGDIIMTLDGHPVEETEDLLARLTGD
                     RVGHPAEVGVLRGGANETLEVTVGERASRR"
     gene            1123..1761
                     /locus_tag="VFJ72_15275"
     CDS             1123..1761
                     /locus_tag="VFJ72_15275"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7480864.1"
                     /translation="MKVFVVASAPVARAGLRAMLAEEDGVEVVGAADSFDAPGFSGAD
                     VVLVADEALVPEAVGDLAEYRSQGLLVLSDDEGAVAMLRGTGPGGWGMLAPDAPPEEI
                     SAAIQAVGNGLVVLPEALAERLLGAPSASGVMDGDGGGDLMEPLAEPLTAREREVLDL
                     LGQGLSNRLIARELHISEHTVKFHVSSVYAKLGVGSRAGAVSQGARRGLISL"
     gene            1835..2590
                     /locus_tag="VFJ72_15280"
     CDS             1835..2590
                     /locus_tag="VFJ72_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480865.1"
                     /translation="MRTPGMLLLVLLACISLGCGGGRDASSPESAPDARSGPSAAGQR
                     AERQEPQPATKVIVTSDDAALPDGCHPREAALLVSDFINAFNRGDGEALGRVFFISEG
                     PSPPDFSDTSVYPWSWYSVSQVGDGGKIEDGFTTYDQGELLRYFAWRHERGERLRLLK
                     VGLTGPGMLGERGNVGIVYVLTRTAGDLKAGMGGPDGVAFGRGAINCATRRIFAWSME
                     MLAGETRGSRAASEWLCSNPPGWRPGDAVVACA"
     gene            complement(2674..3741)
                     /locus_tag="VFJ72_15285"
     CDS             complement(2674..3741)
                     /locus_tag="VFJ72_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET7480866.1"
                     /translation="MKAIQLPDDHNLFHTLDFDLPPDLEASEPPEARGLARDEVRLMV
                     SYRRSDRVVHSTFRNLPDFLDAGDALVINTSGTMNAALSARRADGTPLELRLSTSLPD
                     GLRAVEVRRPVGTSTEPFRRARAGETLRLPAGGMATLQAPYVSGSRLWTSTLDLPEPL
                     DEYLEHHGFPIRYGYVREDWPLDYYQTVYATERGSAEMPSAGRAFTAELIARLVANGV
                     QVAPLLLHTGVASLEDDEPPYEEHYRVPPETARLVNAAHASGRRVVAVGTTVVRALET
                     VTGEDGVTRPGEGWTGLIVTPERGVRAVDALLTGLHEPRSTHLAMLEAVAGREHLEVA
                     YEEALRSGYLWHEFGDLHLIL"
     gene            complement(3743..4885)
                     /locus_tag="VFJ72_15290"
     CDS             complement(3743..4885)
                     /locus_tag="VFJ72_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7480867.1"
                     /translation="MNDDLFRTALVTGASRGLGLALARRLAADGWNLIIDARGAEALE
                     AVRAELADLTNVTAIPGDVTDAEHRRKLAEAAREAGGLDVVVNNASILGPSPQPNLLD
                     YPLDVLEEVYRANTVAPLAVIQALKDVLKPGARILNITSDAAVEPYPGWGGYGSSKAA
                     LEQISNILAAENPELRVYHVDPGDMRTQLHQEAFPDEDISDRPLPDESVPGLLALISD
                     DLPSGRYQARNLTETDNSPEKLRQLRVALTVDDFDRAARVYRDAFGLAVEKEWSRPEG
                     RGIILRAGPASIELVDHSQAAYIDEVEADEIEAGGHVSGPVRLAFEVEAPGSTAEALQ
                     EGGAKALGGEVETPWGHLNRRLQTAEGLQITLYRSPDRETAGREVV"
BASE COUNT          816 a         1624 c         1922 g          959 t
ORIGIN      
        1 tccccggccc ttcttctttg tccgcgaact agccgtttct ccagttataa actagccgaa
       61 cgagggatgg cggtctctct tgcgagggat acattatctc cagatggaac ctgatctgga
      121 gatgagtatg gagcgtagag aagagagcct ggccgctctg gcggctctat cggatgggat
      181 ggccgcgctc gtggagcaga ccgggcctgc gatagtgcgg gtgcacggac ggcggcggcc
      241 ttcgagtggt gtggtgttca cgccggagat ggtgctggcg gcgagccacg ccctggagcg
      301 agaggaagac ctgtcggtcg ggactcatga cggtagaacg ctgggggcgc ggctgatcgg
      361 tcgggaccct gcaacggacc tggcggtact gaaggtggag ggtctggcgg tcgaggcggc
      421 gaggctggcg gagggagaag cccgggtggg gcagctctcg ctggcggtcg ggcgatccgg
      481 gcggggagag ggaccgattg tctccgtggg tgtgatcagc gccgtcgggg ggccgttgcg
      541 gatgaggtgg gggatgaggt tggagcggta cataaggacc gatgctcagg tgttccccgg
      601 actctccgga ggggcgctgg ttgatgcgcg gggcggtgtg ctgggtgtgc tcacgaccgg
      661 gctggcgaga ggagtgtcgc tggctgtccc ggcggagctt gcctggcggg tggccggtga
      721 gatctccgag cgcggttcga tccgccgcgg gtacctcggt atcctgagcc agccggtccg
      781 tctgccggac gcccagcggt cggggctgtc tcagagggac ggcctgctgg tcgtcggcgt
      841 cgaggatggt agccccgcag ccagcggcgg catgatggtc ggagacatca tcatgacgct
      901 ggacggccat ccggtcgagg agaccgagga cctgctcgcg cgtctgaccg gcgatagggt
      961 gggccatccg gcggaggtcg gcgtgctgcg cggcggagcg aatgagacgc tcgaagtcac
     1021 ggtcggcgag cgagcctccc ggcgctgaac gtcgaaagct cccggcggcg tccgcgtgag
     1081 cccggacgcc gcgcgagcag agtgggcggt atgatgccgg gcatgaaggt gttcgtagtg
     1141 gcgtcggcgc cggtcgcgcg ggcggggctg cgcgccatgc tcgcggagga ggacggcgtc
     1201 gaggtcgtgg gcgcggccga ctcgttcgac gcgccaggtt tctccggggc tgatgtcgtg
     1261 cttgtcgcgg acgaggccct ggtcccggag gccgtgggag atctggccga gtaccgttct
     1321 cagggactac tcgtactctc tgatgatgaa ggcgcggtag ccatgctccg cggaacgggg
     1381 ccgggaggat ggggcatgtt ggccccggat gcgccgccgg aggagatctc ggcggccatc
     1441 caggccgtcg gtaacgggct cgtagtcctc cccgaagccc tggcggagcg tctgctcggc
     1501 gcgccgagcg cgtcgggcgt gatggacgga gacggcgggg gcgatctcat ggagcccctc
     1561 gcggaacctc tgacagcgcg ggagcgcgag gtgctggatc tcctcggcca gggcctctcc
     1621 aacaggctca tcgcgcgcga gctacacatc agcgagcata ccgtgaagtt ccacgtctcc
     1681 tccgtgtacg ccaagctcgg cgtgggcagc cgggctggcg cggtgagcca gggcgcccgg
     1741 cgcggtctga tctcgctcta accctcccgc taccgcgcac tgccgggtat agaatgaggt
     1801 cctatcgggg ctgtacggag gtgcgagagc ggatatgagg acgcccggaa tgcttctcct
     1861 ggtgctcctc gcgtgcattt cgctgggatg cggcggagga cgggacgcct cttcgccaga
     1921 gtcggcgccg gatgcgcgct cgggaccctc cgctgccgga cagagagcgg agcggcagga
     1981 accacaaccc gccacgaagg tcatcgtaac cagcgacgac gccgcgcttc cggacggatg
     2041 ccatccgcgg gaggcggcgt tgctggtctc ggacttcatc aacgccttca accgcggcga
     2101 tggggaggcg ttgggccgcg tcttctttat ctcggaaggg ccgagtccgc cggacttctc
     2161 cgataccagt gtctatccgt ggtcctggta ctcggtcagc caggtggggg acggaggcaa
     2221 gatcgaggac ggcttcacga cctacgatca gggcgaactc ctgcgctact tcgcctggcg
     2281 gcacgagcgg ggggagcgcc tgcggctgct caaggtcggg ctgaccggac cggggatgct
     2341 cggagagcgg ggcaacgtgg gcatcgtcta cgttctcacc cgtaccgccg gcgacctgaa
     2401 ggccgggatg ggaggcccgg atggcgtggc gttcggcagg ggcgccataa actgcgcgac
     2461 gcgcaggatc tttgcctgga gcatggagat gctcgcgggg gagaccagag gctctcgcgc
     2521 cgcctccgag tggctgtgct ctaacccgcc cggctggcgt ccgggagacg cggtggtcgc
     2581 ctgcgcctga tatagagatt cacaggatag cggttacggg gcgatcctcg tgtccgctca
     2641 cgtcatcccg cgctctctac gctgcggggc tacctacagg atcaggtgca ggtccccgaa
     2701 ctcgtgccag agatatccgc tccggagggc ttcttcgtag gcaacctcca ggtgctctct
     2761 ccccgctacc gcttccagca tcgccaggtg tgtcgaacgg ggctcgtgta gcccggtgag
     2821 cagcgcgtcc acagcccgta ccccccgctc cggcgtgacg atcaggcccg tccagccctc
     2881 gccggggcgc gttacgccat cctcgccggt tacggtctcc agcgcccgca cgaccgtggt
     2941 cccgacggcc acgacgcgcc tgccgctggc atgggcggcg ttcacgagac gggcagtctc
     3001 cggggggaca cggtagtgct cctcataggg aggctcgtcg tcttcgagac tggcgacgcc
     3061 ggtgtgcagg agcaggggag ccacctggac gccgtttgcg acgaggcgtg cgataagttc
     3121 cgccgtgaaa gccctcccgg ctgaaggcat ctcggcgctg cctctctccg tcgcgtagac
     3181 ggtctggtag tagtcgagcg gccaatcttc tctgacatag ccgtaccgga tcgggaaccc
     3241 atgatgttcg agatactcgt cgagcggttc cggcaggtcg agggtcgagg tccacagccg
     3301 cgacccggag acgtagggag cttgaagcgt tgccataccg cctgccggca accggagcgt
     3361 ctcgccggct cgcgcgcgcc ggaaaggctc cgtgctcgta ccaaccgggc ggcgaacctc
     3421 caccgccctg agcccgtctg gcaggctggt ggagagccgc agttcgagcg gcgtgccgtc
     3481 cgcgcgccgg gcagagaggg cggcgttcat cgtgccgctg gtgttgatga ccagcgcatc
     3541 tccggcgtcg aggaagtccg gtaagttccg gaaggtagag tggaccacgc ggtcgctcct
     3601 acggtaagag accatcaggc gtacctcgtc gcgcgcgagg ccgcgggctt cgggcggctc
     3661 gctcgcctcc aggtccgggg gcaggtcgaa gtccagcgtg tggaagaggt tgtgatcgtc
     3721 ggggagctgg atcgccttca attcagacta cttcccttcc ggcggtctcc ctgtcgggag
     3781 accggtagag cgttatctgc aagccctcgg cggtctggag ccgccggttg aggtggcccc
     3841 agggcgtctc gacctcgcca ccaagagcct tcgcgccacc ttcttgtagc gcctcggcgg
     3901 ttgacccggg ggcctcgacc tcgaaggcca ggcgcaccgg gccggagacg tgcccgccgg
     3961 cttcgatctc gtctgcttcg acctcgtcta tataggcagc ctgcgaatga tctaccagct
     4021 cgatgctcgc gggaccggct cgcaggatga tcccccgccc ttccgggcgc gaccactctt
     4081 tctccacagc cagcccgaag gcgtccctgt aaacgcgcgc cgcccggtcg aagtcgtcta
     4141 ccgtgagcgc gacgcgcagt tgccgcaact tttctggact attgtccgtt tcagtgaggt
     4201 tgcgagcctg gtagcggcca ctcgggaggt catccgagat caaggccagc aggcccggca
     4261 cgctctcatc tggtagcggt cggtcgctaa tgtcctcgtc cgggaaagcc tcctggtgca
     4321 actgggtcct catgtcgccg gggtccacgt ggtagacgcg caactccggg ttctcggcgg
     4381 cgaggatgtt ggagatctgc tcgagcgcgg ccttgctgga gccgtagccg ccccatcccg
     4441 gatacggctc gacggccgcg tcactggtga tattcaggat acgggcgccg ggcttcagta
     4501 cgtctttgag cgcctggatc acggcgagcg gcgctaccgt gttcgcccgg tagacctcct
     4561 ccagcacgtc cagcgggtag tccaggaggt tcggctgcgg gctgggtccg aggatgctcg
     4621 cgttgttgac gactacgtcc agaccgccgg cttcccgcgc ggcctcggca agtttccggc
     4681 gatgctccgc atcggtgacg tcgccgggga tcgcggttac gttggtaagg tccgcgagtt
     4741 ccgcgcggac cgcttcgagc gcttcggcgc cgcgggcgtc aattatgagg ttccagccgt
     4801 cggccgcgag ccgccgggcc agcgcgaggc cgaggccgcg cgaagcgccg gttacgaggg
     4861 ccgtgcggaa gagatcgtcg ttcatggcgt gcctccgcgt ccatcatctg tccgttggta
     4921 tttgaataga atgtatcgtg ctgtaggtct cctggaatcg ccaggacgga cggtcttccg
     4981 tctgtacttt cgtacaggtt ccgtgagact ctacgagtgt gttcgagggt gtcaggctag
     5041 tagctctcgg atgttctgag gcgaggcggc tacgttgtcg tccgtatcga gatgagactg
     5101 gtacacatgg gtctcaccaa ctcccatccc acgcggatgt cgcttgccgc aatactggac
     5161 gaagtctatg atgtaacagc aggtacgaag ctctgattat tggttaagaa cgcagcggag
     5221 gaaagctatg gtccagcagg aagcggtctc ggttgaaacg ttgaaggcaa tcaccaatgc
     5281 gttcaacgct catgatctgg acgcgatcat ggggttcttc g
//
LOCUS       DASSMB010000267         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_855017, whole genome shotgun sequence.
ACCESSION   DASSMB010000267 DASSMB010000000
VERSION     DASSMB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855017"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VFJ72_15295"
     CDS             <1..990
                     /locus_tag="VFJ72_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET7480868.1"
                     /translation="KAEKCTLCYPRIEAGQPTICSETCVGRLRYIGLILYDADAVEEA
                     ASVPDEKDLLEAQRRVFLDPNDPGVVEQARLDGVPDDWISFAQRSPIYRLAIEWGIAL
                     PLHPEYRTLPMVWYVPPLSPVMGFVEGEGSEADPDDVFPAIDELRIPIKYLANILSAG
                     DEEVVRRVLKQLAAMRRYMREKNLSGTVDASVAASVGMSPQAIEDMYRLLAIAKYEDR
                     YVIPLAHRELAHELYEQRGAAGLDFAGVRAPAAGGEHNAAYSCSLDFEGGPGSCATTA
                     SGGGRNEAFYAMKGELEKRARELGGKVRAPNVSFFSSKADFEAFHLKGEGSGA"
     gene            987..1622
                     /gene="narJ"
                     /locus_tag="VFJ72_15300"
     CDS             987..1622
                     /gene="narJ"
                     /locus_tag="VFJ72_15300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HET7480869.1"
                     /translation="MIFFKLLSILLRYPDDEVLEHRNEIAEAVCELPDSPAKDAILRF
                     LDYWGNEPPQRLQERYIAAFDFNRRGCLYLSYYRYGDMRARGQALVDMKSVYERSGYP
                     LDTTELPDYLPLVLEFASEDLEEGLAMIAEHCAAVEVIRRSLRHDRSPYAHLIEALGF
                     FLPRIDAQTEMEMRKLIVQGPPQETVGLEPYTADAPAGGALGNVAFYGRKG"
     gene            1619..2395
                     /gene="narI"
                     /locus_tag="VFJ72_15305"
     CDS             1619..2395
                     /gene="narI"
                     /locus_tag="VFJ72_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HET7480870.1"
                     /translation="MSLWDQFLWVIFPYLALGTFVLGHVYRYVYDQYGWTPKSSQFLE
                     RRLLMWGVLLFHWGFLFVLAGHVMGLLVPIGVYRSLGVTDESYHFLSLWAGSVAGVAA
                     MVGILLLNVRRWFVGRVRNNTDTMRFLTDAVLMLVILLGLAATIGYRVYVSQYELPHA
                     FEYRENIGPWFRSLFAFSPDPQLMVGAPLIFQIHTLSAFLLFALWPFSSLVHVWSVPL
                     GYLRRSYIQYRSSNPRAALDRERARKGDGNAPAKRQGRTF"
     gene            2419..>2892
                     /locus_tag="VFJ72_15310"
     CDS             2419..>2892
                     /locus_tag="VFJ72_15310"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7480871.1"
                     /translation="MAEENGHRGMERDSRAVAEAARESRWERPSFGKELFLGRFRPDL
                     IHPHPEPDPEEQRRAAEFIGKLEGFLRENVDAEEIDRAGKVPDRVVKGLAELGAFGIK
                     IPEEYGGLGLSQFSYNRAMGLVASSSAALVTLLSAHQSIGVPQPLKLFGTGEQKRK"
BASE COUNT          505 a          913 c          953 g          521 t
ORIGIN      
        1 aaggccgaga aatgcacttt atgctacccg cgcatcgagg ccggtcagcc gaccatctgc
       61 tccgagacct gcgtcggcag gctgcgttac attggattga tactttatga cgcagacgcg
      121 gtcgaggaag cggcctccgt cccggatgag aaggatctgc tcgaagcgca gaggcgggtc
      181 ttcctcgacc ccaacgatcc gggagtcgtg gagcaggcgc gcctggacgg ggtgccggac
      241 gattggatct ccttcgccca gcgatccccg atctaccgtc tggctataga gtggggcatc
      301 gcgctcccgc tccaccccga gtaccggacc ctccctatgg tctggtacgt gccgccgctc
      361 tctccagtga tgggcttcgt cgagggcgag ggctcggagg ccgacccgga cgacgtcttt
      421 ccggccatag acgagcttcg aatcccgata aagtacctgg cgaacatcct atcggcggga
      481 gacgaggagg tcgtacgccg cgtcctcaag cagctcgccg ccatgcgccg ctacatgcgt
      541 gagaagaacc tctcgggtac ggtggacgcg agcgtggccg cctccgtcgg catgagcccg
      601 caggccatag aagacatgta ccgcctgctc gccatcgcga agtacgagga ccgctacgtc
      661 atcccgctcg cccacaggga gctcgcccac gagctatacg agcagcgcgg cgccgccggc
      721 ctggacttcg ccggcgtccg cgctccagcc gccggcgggg agcacaacgc ggcatactcc
      781 tgcagcctgg acttcgaggg cggccccgga agctgcgcca ccaccgcgag cggcggaggc
      841 cgcaacgagg cgttctacgc catgaaggga gagctggaga agagggctcg cgaactcggc
      901 gggaaggtgc gggcgccgaa cgtcagtttc tttagtagca aggcggactt cgaggcgttt
      961 catctcaagg gagaggggtc gggcgcgtga tcttcttcaa gctcctctcc atcctgctgc
     1021 gctaccctga tgacgaggtc cttgagcatc ggaatgagat agcggaggcc gtgtgcgaac
     1081 tgcccgactc tcccgcgaag gacgccatac tccggttcct tgattactgg gggaacgagc
     1141 cgccgcagcg attgcaggaa cgctacatcg ccgccttcga cttcaaccgg cggggctgcc
     1201 tgtacctgag ctactaccgc tacggggaca tgcgggcgcg cgggcaggcc ctcgtggaca
     1261 tgaagagcgt ctacgagcgc tccggttacc cgcttgatac caccgagctg ccggattacc
     1321 tcccgctcgt gctggagttt gcctccgagg atctggagga gggactggcg atgatcgcgg
     1381 aacactgtgc agccgtcgag gtgatccggc gctccctgcg ccacgaccgc agcccatatg
     1441 ctcatctcat cgaggccctg ggcttcttcc tgccgcggat agacgcgcag acggagatgg
     1501 agatgcggaa gctcatcgtg cagggaccgc cgcaggagac ggtcgggctg gagccgtaca
     1561 ccgctgatgc tccggcggga ggcgctttgg gcaacgtcgc gttttatggg aggaagggat
     1621 gagcctctgg gaccagttct tgtgggtcat attcccctat ctcgccctgg gcaccttcgt
     1681 gctcgggcac gtctaccgct atgtctacga ccagtacggc tggaccccga agtccagcca
     1741 gttcctggag cgccggctcc ttatgtgggg cgtcctcctg ttccactggg ggttcctatt
     1801 cgtcctggcc gggcacgtga tggggctttt ggtaccgata ggcgtctaca ggtcgctcgg
     1861 cgtcacggac gagtcgtacc acttcctctc cctgtgggcc ggttcggtgg ccggagtagc
     1921 ggcgatggtc ggcatcctgc tgttgaacgt tcggcgctgg ttcgtcgggc gcgtcaggaa
     1981 caataccgat accatgcggt tcttgacgga cgcggtgctg atgttggtga tcctgctggg
     2041 actcgcggcg accatcggat acagggtgta cgtcagccag tacgagcttc cgcacgcctt
     2101 cgagtaccgc gagaacatcg gaccctggtt ccgtagcctc ttcgcgttct cgccggaccc
     2161 gcagctcatg gtcggggcgc ccctgatctt ccagatccac accctctcgg catttttgct
     2221 attcgccctc tggccctttt ccagcctggt ccacgtctgg agcgtgcctc tcggctacct
     2281 gcgccgcagc tacatccagt atcgcagctc caatccccgc gcggccctgg accgcgaacg
     2341 cgcccgcaag ggggacggta atgctcccgc caagaggcag ggcaggacat tttagacggc
     2401 ctcaatccgg agacgatcat ggctgaagag aacggacata gaggcatgga acgcgactct
     2461 cgtgcggtcg cagaagcggc gcgggagtcc aggtgggagc gtccgagctt cgggaaagag
     2521 ctgttcctgg ggcggttccg gccggacctg atccacccgc accccgagcc ggaccccgag
     2581 gagcagcgcc gggccgcgga gttcatagga aagctggagg gtttcctgcg ggagaacgtg
     2641 gacgcggaag agatagaccg cgccggaaag gtgccggaca gagtcgtaaa ggggctcgcg
     2701 gagcttggcg cattcgggat caagatcccc gaagagtacg gcggactggg actctcccag
     2761 ttcagctaca accgggcgat ggggctggta gcttcctcat cggcggcgct cgtcaccctg
     2821 ctctcggcgc accagtccat cggcgtgccg cagcctttga agctcttcgg gaccggagag
     2881 cagaagcgga ag
//
LOCUS       DASSMB010000268         4809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1354402, whole genome shotgun sequence.
ACCESSION   DASSMB010000268 DASSMB010000000
VERSION     DASSMB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354402"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="VFJ72_15315"
     CDS             complement(<1..615)
                     /locus_tag="VFJ72_15315"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET7480872.1"
                     /translation="MGKHSRVKKFRHRVGFFLFASLCLVAGAFAVWYLILAPDGSSKT
                     EPFNKADTPKVAQAPKPAKKEAASKPAMVKVPEIPKGTTEAKAKALLKSKDLKLGEVT
                     KKSSDDVDKGGVISQDPAPKKEVKEGSAVDIAVSSGPKEPAKNSEKGSKDISVANVPA
                     PPSTAMTLTVPAMGKSGEPIGEGVDEGTLYNGPGHAPGTGYPWIA"
     gene            complement(741..1100)
                     /locus_tag="VFJ72_15320"
     CDS             complement(741..1100)
                     /locus_tag="VFJ72_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480873.1"
                     /translation="MPPTVQVLYFEGCPGGAASREALREALPEVKIDMVELPPREFRK
                     LGIPGSPTLLLNGRDLFPAPPSDNRSIEIDTGPSRCRLYGTPEGLKSHPTANMIRQAF
                     EESATGTAAASEPGDTG"
     gene            complement(1135..1449)
                     /locus_tag="VFJ72_15325"
     CDS             complement(1135..1449)
                     /locus_tag="VFJ72_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7480874.1"
                     /translation="MCTLAKFFNGFANSTRLSILLLLSRQGETKVGDLVEALGAPQPR
                     VSDHLRCLSWCGYVTVRREGRNAYYSVTDPRVMEMLELGESLLRDNLEHVEACDVTRK
                     GC"
     gene            complement(1671..3491)
                     /locus_tag="VFJ72_15330"
     CDS             complement(1671..3491)
                     /locus_tag="VFJ72_15330"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HET7480875.1"
                     /translation="MGLRFLLAVLMFSALAPLLAPAASAQENRVFDQADVLSKAQESK
                     VLSAFDKTSADSGDPIYAFLVNDTNVAPADRPDLLMEEANKAGAPDNADVILVAPDDR
                     WGLVDVSGGSDQAVYDAMAPSFADGKYADGLIAGAAQIQDSLSIVPEVLTTIGVLGGL
                     AALAGGTTFVLFRRRKQQRLEEERQAAEEEFAGLTTRIDEFDEKERLVSGYLEVQRPL
                     LDQRTEEWIEERISEAKTAGFASEFNEAAAELVSDPFSARQRLARGRELLDGALARLD
                     EAERTMDDYRAADEVLDGRLRAVAEEIASAEETEASVRAEGVSVRPLDLRPEYDRLAK
                     EAAERAARRDEFDPRKALAAADDLVERARERRESLRAEVEARDALPEERRSCEDALGR
                     ARQTLSDYDGAYKSALREWGPAALGDAPNPGELSSGLRQAEGCMESADRAADSGSFAK
                     ARSLLQQSSGLAHGISGAPAALRAATVEADRKKREGEERLRELEERLEQARANEYRMS
                     PVQQRQLREYERRLEDARGGFFGGDWLTAILLFEALDTDYVYMDDPSYMAGDGGSGDG
                     GDWSGWGDGGGSGGGGDWGGGDFGGGGDFGGGGDFGGGGW"
     gene            complement(3576..4484)
                     /locus_tag="VFJ72_15335"
     CDS             complement(3576..4484)
                     /locus_tag="VFJ72_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta(1)-pyrroline-2-carboxylate reductase
                     family protein"
                     /protein_id="HET7480876.1"
                     /translation="MKVLGPEETASRLPYAELADSIREVALSSKAHTPPRLALPLSED
                     GVLLAMPAADSDIAITKLVTVHPNNAERGLPTIQGEVVVMEAGTGERLGVLDGATVTA
                     RRTAALSLLAAREFAPHPDGPLLIVGAGTQGRSHLEAFREGLGVSQVFITSRTADSAA
                     KLAEYAESLGMEARTVDGPGEALEEVSLIVTATTSREPVLPEEVPDGVFVAAVGAFKP
                     EMAELPLALISRSTVVVDTLEGAKEEAGDLLAAEQAGAFRWGDAATLEQALRWSGPPG
                     GTVVFKSVGLALWDLAAARTAFLPRQ"
     gene            complement(4487..>4809)
                     /locus_tag="VFJ72_15340"
     CDS             complement(4487..>4809)
                     /locus_tag="VFJ72_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7480877.1"
                     /translation="PETIDREVHGEEVEFIRRYLARHVPALNGDLLHTKTCMYTNTPD
                     EHFVICAHPEHPQVSVAAGFSGHGYKFCSVVGEILADLATEGATSHPIDLFSPTRLKD
                     AARG"
BASE COUNT          761 a         1581 c         1556 g          911 t
ORIGIN      
        1 ggcgatccag gggtatccgg tgcctggggc gtgtccggga ccgttgtaga gggttccctc
       61 gtcaacgccc tctccgatag gctcgcctga cttacccatc gcgggaaccg taagcgtcat
      121 cgccgtgctg ggcggcgcgg gaacgttcgc caccgaaatg tcctttgagc ctttctccga
      181 attcttcgct ggttccttcg gaccggagct caccgcgata tccacggcgc tgccttcctt
      241 gacctctttc ttgggcgccg ggtcctgaga gatcacgccg cccttgtcca catcatcgct
      301 cgacttcttg gtcacctcgc cgagcttgag atccttgctc ttgagcagag ccttggcctt
      361 cgcctccgtc gtccccttcg ggatctccgg gaccttgacc atggcgggct tggaagcggc
      421 ttctttcttc gccggcttgg gagcctgagc caccttggga gtatcagcct tgttgaacgg
      481 ctcggtcttg ctcgacccat caggcgccag aatgagatac cagacggcga aagcgccggc
      541 aaccagacat agagaggcga acaggaagaa ccctacccga tgcctgaact tttttactct
      601 ggagtgtttg cccatatatc cgtggtcctt tccccgcttc gaccgccgtt gtagattgag
      661 tttcgcttac caggattgta tcaggataga tgtatcagac ggaagagatg ttgcgaagat
      721 ttaatatttt ttgtgaattg ttaaccggtg tctcctggtt ctgacgcggc cgccgtgccg
      781 gtggcggact cttcgaaggc ctgccgtatc atgttcgctg tcggatggct cttcaggccc
      841 tcgggcgtgc cgtagagtct gcaacgcgaa gggccggtgt cgatttcgat gctccggtta
      901 tcggaaggag gagcggggaa gaggtctctg ccgttcagga ggagggtggg gctgccaggg
      961 atgccgagct tgcggaactc tcgcggaggt agctcgacca tatcgatctt gacctctggg
     1021 agcgcctcgc gcagcgcctc tctggaggcc gctccgcccg gacagccctc gaagtagagt
     1081 acctgtacag tgggcggcaa cggagagctt tcggaggcgg agcgtaggat accgctaaca
     1141 acctttacga gtgacatcgc aggcctctac gtgttccagg ttgtctctaa gcagcgactc
     1201 gcccagctcc agcatctcca tcacgcgcgg atcggtgacc gagtagtatg cgtttcggcc
     1261 ctcgcgccgg accgtgacgt aaccgcacca ggagagacag cggagatggt ccgagacccg
     1321 cggctgcggc gccccgaggg cttcgactag atccccgacc ttcgtctctc cctggcgaga
     1381 caggaggagc aggatcgaga gccgcgtgga attggcgaag ccgttgaaga actttgccag
     1441 agtacacatc tgttcactat cgcgacctac cgggacgtcc gtaagagaac ccgttctaat
     1501 cacctcttcc tgcacgttca ctcctctcag accgtgactg tccgggacct gtaccccgaa
     1561 gttcgtatat gtgccggctg acatactcct gccgaggaga agatgtcccc gattgccgtg
     1621 actttatagt ataggccggt aacaggttgc acaatgccgt ctcggcgctg ttaccagcct
     1681 ccgccgccga aatctccccc gccgccgaag tcgccgccgc caccgaaatc tccgccaccc
     1741 cagtcgccgc ctccgccact tcctccgccg tcgccccagc cggaccagtc tccgccatcg
     1801 ccggaacctc cgtctcccgc catgtaggaa gggtcgtcca tgtagacgta gtcggtgtcg
     1861 agagcctcga agagcaggat cgcggtgagc cagtcacccc cgaagaagcc gccgcgcgca
     1921 tcttccaggc ggcgctcgta ctctcggagc tgccgttgct gaacggggct catgcggtac
     1981 tcgttggctc tggcctgctc caggcgctct tccagctccc ggagcctttc ttcgccctca
     2041 cgcttcttgc ggtcggcctc gacggtcgcg gctctcaggg cggcgggagc acccgagatt
     2101 ccgtgcgcga gccctgatga ttgttgcagc agggaccggg ccttggcgaa gctgccggaa
     2161 tctgcggcac gatccgcgct ctccatgcag ccctcggcct gccgcagacc ggacgagagc
     2221 tcgccggggt tgggcgcatc cccgagcgcg gccggacccc actcccggag ggcggactta
     2281 taagcgccgt cgtaatcgct taaggtctgc cgggctctgc cgagcgcgtc ctcgcacgag
     2341 cggcgctcct ccggcagcgc gtcgcgggcc tcgacctcgg cgcgcagcga ctcccggcgc
     2401 tcgcgggcgc gctctacgag gtcgtcggcg gcggcaagag ctttgcgggg atcgaactcg
     2461 tcccggcggg cggcgcgctc cgccgcttcc tttgccagac ggtcgtactc cgggcgcagg
     2521 tcgagcggcc gcacggatac accttcggcc cggacagagg cttcggtctc ctcggcgctc
     2581 gctatctctt cggccaccgc tcgcagccgg ccgtcgagga cctcgtcggc ggctcggtag
     2641 tcgtccatag tcctctcggc ctcgtcgagt ctggcgagcg cgccgtcgag cagctcccgt
     2701 ccgcgcgcca gacgctgtcg ggcggagaag ggatcggata cgagctcggc cgcggcctcg
     2761 ttgaactcgc tcgcgaagcc ggcggtctta gcctcggaga tgcgctcttc gatccattcc
     2821 tccgtccgct ggtccagtag cggacgctgg acctccaggt agccggagac gagccgctcc
     2881 ttctcgtcga actcatctat ccgggtcgtc aggccggcga actcctcttc agccgcctgg
     2941 cgttcttctt cgagccgctg ctgcttgcgg cggcggaaca ggacgaaggt tgtgccgccg
     3001 gcgagggcag ccagccctcc gagcacgccg atggtggtga ggacctccgg cacgatgctg
     3061 agtgagtcct gtatctgtgc ggctccggcg ataaggccgt cggcgtattt accgtcggcg
     3121 aagcttggcg ccatggcgtc gtagacggcc tgatccgacc cgccggacac atccacgaga
     3181 ccccagcggt catcgggagc aacgaggatg acgtccgcgt tgtccggcgc tccagccttg
     3241 ttggcctctt ccatgaggag gtccggacgg tcggcgggag cgacgttggt gtcgttgacg
     3301 aggaaagcgt agatcgggtc tcccgaatcg gcgctcgtct tgtcgaaggc gctcaagacc
     3361 ttgctctcct gagccttgct cagtacgtcg gcctggtcga agaccctgtt ctcctgggcc
     3421 gaagcggcgg gagcgagcag gggcgcgagc gccgagaaca tcagcaccgc tagaaggaac
     3481 ctgagaccca ttcgccctcc tcgtgtttgc ccggataact gattatacgg gccgcgcgcc
     3541 gcgaggttgc aacccaggca tggcggcgcc agcacctact gtcgcggcag aaacgccgtc
     3601 cgggccgcgg cgaggtccca tagagcgagg cccacgctct tgaacactac cgtcccgccc
     3661 ggaggtccgg accaccggag cgcctgttcg agcgtcgcgg catcccccca ccggaaggct
     3721 ccggcttgct ccgcggccag caggtcaccg gcttcttctt tcgcgccctc cagagtatcc
     3781 acgacgacgg tggagcgcga gatcaaagcc agcggaagct ccgccatctc cggcttgaac
     3841 gcgccgaccg ccgccacgaa cacgccatcc ggtacttcct ccggcaggac cggctcccgg
     3901 ctggtcgtcg cggtgacgat gaggctcacc tcttccagag cctctcccgg cccatcgacc
     3961 gttcgagcct ccatccccag gctctccgcg tactccgcca gcttcgcggc gctatcggcg
     4021 gtgcgggagg ttatgaagac ctgcgagacc ccgagcccct ctcggaaggc ttcgaggtgg
     4081 gagcgtccct gagtgccggc gcccacgatc aggagcggac catcggggtg cggcgcgaac
     4141 tctcgggcgg cgagcaggct gagcgccgcg gtgcgccggg cggtgacggt tgcgccgtcc
     4201 aggacgccga gtcgttcacc cgtcccggcc tccatgacga ccacctcgcc ctggatggtc
     4261 ggcaggccgc gctccgcgtt attcgggtgg accgtgacga gcttggtgat ggctatgtcg
     4321 gagtcggcgg cgggcatggc gagaaggacg ccgtcttcgg agagtggcag cgctaggcgt
     4381 ggcggggtat gggctttgct ggagagcgcg acctcgcgga tagaatcggc cagctctgcg
     4441 taaggcaggc gcgaggcggt ctcttcaggg ccgaggacct tcacgactag ccgcgcgcgg
     4501 cgtccttcag gcgggtgggg gagaagaggt ctatcgggtg ggatgtcgcg ccctcggtgg
     4561 cgaggtcggc caggatctca cccaccacgc tgcagaactt gtagccgtgt cccgagaagc
     4621 cggcggctac ggagacctgg ggatgttccg gatgcgcgca gatgacgaag tgctcgtcgg
     4681 gggtgttagt gtacatgcag gtcttggtgt gaaggagatc tccgttcaga gccgggacgt
     4741 ggcgagcgag gtagcgccgg atgaactcca cctcctcgcc gtgcacctca cggtctattg
     4801 tctccgggt
//
LOCUS       DASSMB010000269         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_101176, whole genome shotgun sequence.
ACCESSION   DASSMB010000269 DASSMB010000000
VERSION     DASSMB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101176"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFJ72_15345"
     CDS             complement(<1..401)
                     /locus_tag="VFJ72_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7480878.1"
                     /translation="MPGFGDPEASVVIFGLAPAAHGANRTGRFFTGDRSGDFLFAALH
                     RTGFANQPVSRGRDDGMRLSGAWISAAVRCAPPQNKPTPAERDACLPYSARELELIGA
                     KVIVCLGAFAWDAALRLYKVRPKPKFGHGAE"
     gene            complement(573..1781)
                     /locus_tag="VFJ72_15350"
     CDS             complement(573..1781)
                     /locus_tag="VFJ72_15350"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HET7480879.1"
                     /translation="MNLDLMRELSVSTDSKIVLLVLDGLGGLPMEAGGPTELEAASTP
                     NLDRLAATSDLGLSLPVAAGVSPGSGPGHLALFGYSPLEYVVGRGVLSALGVGFDLGR
                     DDLAARINFATLDDSGKISDRRAGRIPSEKGSELVDLLNSKIDLDGVEVFITHEKEYR
                     AVVVFRAEGLSGSLADTDPQRTGLEPLPVKPTDDTGEAEKSAKLTNEFVRQANEILKD
                     EHPANTVLLRGFDTHPDLPSFEEAYKLRAAAIASYPMYKGLARLAKMELLKEGEGLSG
                     EFDTLESNWDDHDFFFVHIKPTDAAGEDGDFERKASVIEEVDALIPRLLDLAPDALAI
                     TGDHATPAKMKSHSFHGVPFLLHSDYTLPTAQSFGERVCAGGSLGVFPAEEIMGLLMG
                     HALKLNRYGA"
     gene            1875..>2471
                     /locus_tag="VFJ72_15355"
     CDS             1875..>2471
                     /locus_tag="VFJ72_15355"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7480880.1"
                     /translation="MKIEIIDLNFMNTERVTASFLLLGDSGASAAIVETGPTTCLDHL
                     TSGLKEHGVSHEDVRQVFLTHIHLDHAGASGHLAELLPNATFYVHEIGYPHLVDPSKL
                     VKSATRIYGDRMDELWGEVRPVPEDRIKIIGEGEEIEAAGGVLSAHDTPGHAYHHLAY
                     LEPDSGALFAGDVAGIRLPGQSYVRPPTPPPEIDVDAWI"
BASE COUNT          394 a          791 c          856 g          430 t
ORIGIN      
        1 tactcggcgc cgtgaccgaa ctttggtttg ggacgcactt tgtagaggcg cagggcggcg
       61 tcccaggcga aggctccgag gcagacgatg actttcgcgc cgatgagttc caactcgcgg
      121 gcggagtagg ggaggcaggc atcgcgttcg gcgggggtcg gtttgttctg tggcggggcg
      181 caccggactg cggcggagat ccaggctcca gagagccgca tcccgtcgtc ccggccgcgt
      241 gagaccggct ggttggcgaa gccggtacgg tgcagagccg cgaagaggaa gtcgcccgag
      301 cggtcgccgg tgaagaagcg ccccgtgcgg ttcgcgccgt gggcggcggg tgcgaggccg
      361 aagatcacga cgctcgcctc cgggtcgccg aagccgggca cgggccggcc ccagtaggtt
      421 tcatcccggt gggcggcttt cttctcgcgg gcgaccttct cgcgccattc gaccaggcgc
      481 ggacagaggc ggcaggccgt tatctcacgc tccagcttcg ttagcgcggc gagcgccgga
      541 tgccgttcgc cggcttctat ggtcgttctt ctctaggcgc cgtagcggtt cagcttcagg
      601 gcgtggccca tgaggaggcc catgatctcc tctgccggga acacgccgag agagccgccc
      661 gcgcagacgc gctccccgaa gctctgtgct gtcggcaagg tgtagtccga gtgcaggagg
      721 aacgggacgc cgtggaagga gtggctcttc atcttcgcgg gcgtggcgtg gtcgcccgtg
      781 atggcgagcg cgtcgggggc caggtcgagg aggcgtggga tcagggcgtc cacctcctct
      841 atgacgctcg ccttccgctc gaagtccccg tcctcgccag ccgcgtccgt cggctttatg
      901 tggacgaaga agaagtcgtg gtcgtcccag ttggattcca gggtgtcgaa ctcgcctgag
      961 agcccttcgc cctccttgag aagctccatt ttggcgaggc gggcgaggcc cttgtacatc
     1021 gggtagctcg ctatggcggc ggcccggagc ttgtaagcct cctcgaagga cggcaggtcc
     1081 gggtgcgtgt cgaagccgcg gagcaggacg gtattggccg gatgctcgtc tttcaggatc
     1141 tcgttcgctt gccggacgaa ctcgttcgtc agcttcgcgc tcttctctgc ctcgcccgtg
     1201 tcgtcggtcg gctttacggg gaggggttcg aggcccgtcc tctgcgggtc ggtgtcggcg
     1261 agcgagccgg agaggccctc ggcgcggaac acgactacgg cccggtattc tttctcgtgc
     1321 gtgataaaga cttcgacgcc gtcgaggtct atcttcgagt tcaggaggtc tacgagctcg
     1381 gagcccttct cggagggaat gcgtccggcg cggcggtcgg agatcttgcc ggagtcgtcg
     1441 agggtggcga agttgatcct cgcggccagg tcgtccctgc cgaggtcgaa cccgactcct
     1501 aaagcgctga ggacgccgcg gccgacgacg tactcaagcg ggctgtagcc gaagagcgcg
     1561 aggtgtccgg ggccggagcc ggggctgacg ccggcggcca ccgggagcga gagcccgagg
     1621 tcggaggtgg cggcgagccg gtcgaggttc ggggtgcttg cggcttcaag ctcggtcggg
     1681 ccgcccgcct ccatcggcag accgcccagt ccgtccagga cgagcagcac gatcttcgag
     1741 tccgtcgaga cggatagctc gcgcatgaga tccaggttca cgtcaaagtc ctcctctttc
     1801 gtggtccctc gcggttccgg cagtctagca ggtctgtata atctccgggc cggactttgg
     1861 atcagggagg gaaaatgaag atagagatca tagacctgaa cttcatgaac accgaacggg
     1921 ttacagcctc cttcctccta ctcggcgaca gcggtgcttc ggccgccatc gtcgagaccg
     1981 gcccgacaac gtgcctggac cacctgactt ccggcctgaa ggagcacggc gtctcccacg
     2041 aggacgtgcg gcaggtcttc ttgacccaca tccacctcga ccacgccgga gcctcgggac
     2101 acctggccga gctactgccg aacgcgacgt tctacgtaca cgagatcggc tacccgcacc
     2161 tggtagatcc ttccaagctc gtcaagagcg ccacccgcat ctacggcgac cgcatggacg
     2221 agctatgggg cgaagtgcgc cccgttcccg aagaccgcat caagataatc ggagagggcg
     2281 aggagataga agccgccggc ggcgtcctca gcgcccacga cacgcccgga cacgcctacc
     2341 accacctcgc ctacctggag ccggactccg gcgcgctctt cgccggagac gtggcgggca
     2401 tacgcctgcc cggacagtcc tacgtccgcc cgccgacccc gccgccggag atcgacgtgg
     2461 acgcctggat c
//
LOCUS       DASSMB010000270         8005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_481717, whole genome shotgun sequence.
ACCESSION   DASSMB010000270 DASSMB010000000
VERSION     DASSMB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481717"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFJ72_15360"
     CDS             <1..976
                     /locus_tag="VFJ72_15360"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7480881.1"
                     /translation="LSLHLKSLAMCMDLGIKNLEANQLINCGRLYLSLGAPEDALEHF
                     RHAARLGRETGYARDEGYSLMGVGVCLEHTGRPASAANAYRQAIKLLETSYELSEMPE
                     ELSGKADALVLMAAVLDRSLGRPVDALDAYEEAAEIYSRLGDPLKLRSLLVRRAGLRW
                     RTGNFEGSARDYEEALERARGQGEPAREAAALASLSVVYRDLGRLQESLRSGRAALKL
                     MRDLEDPQAEAYVLSSLAESHEQLGHYPSALSCLKRSLRLRRKIGDREGEVGALHDLA
                     RIYETLGDLDRSSEASDEAISKREALEDGPKEGLKEGREVIATGEGRN"
     gene            977..1240
                     /locus_tag="VFJ72_15365"
     CDS             977..1240
                     /locus_tag="VFJ72_15365"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HET7480882.1"
                     /translation="MPKYIIVRTVGQISEEEIEAASMRSIEALDQMPGVRWIRSYYSA
                     EEGKIYCEYEAPSVELIYEHAQMAGLPVDRCSVVQELEPSMFR"
     gene            complement(1278..2207)
                     /locus_tag="VFJ72_15370"
     CDS             complement(1278..2207)
                     /locus_tag="VFJ72_15370"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7480883.1"
                     /translation="MIDNITLATDGSEDARLAMRAAVDLAERTGAELSVVHAWRVVPL
                     YSNSQDAAWDDYAQIYEREARRVLGSQVDAIEALGGTVASAHLLKSSPIDAILDLCEE
                     TMPDLLVMGSRGRGRLRRALVGSVPEGVAHHIRCPVLVVRGVDGGWPPDRVVAGHDGS
                     EDAQSAAEFAASIGGLFDARCVLVRAHQNPPEPAGGWSAEDRARLDRVALRVEENLKD
                     RAEDLGKTSNKRPEARVIGSDPTLSLLQVAEEEEESRTLLAVGSRGFGVTRRTRLGSV
                     STNLLRVARGPVLIYPHQTMVKRRGKMADREMR"
     gene            complement(2260..3063)
                     /locus_tag="VFJ72_15375"
     CDS             complement(2260..3063)
                     /locus_tag="VFJ72_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7480884.1"
                     /translation="MEATVKVRHKTQEDYGTVPGSAFARKGHQKGEGQAMTTMTEKEE
                     RGAAEDPARLPGAVEALIGARTADAVFVVDPEYRIVHWDAQMESLTGLMAEEVAGGTC
                     HAALSAECEGSGSFCGSRCSVMRLVRAGEAPPSYDMRVSTRWGNKRWVNVSVLSVESD
                     AGPYLIHLMRDSQKMHETLEMARGLIRLSSTRDVPVPDPRDVPALSPRQSEVLGLLAE
                     GKSVKEMCSVLYLSQATVRNHVRSVLQALGAHSQLEALAKARKLGLLSE"
     gene            3337..5556
                     /locus_tag="VFJ72_15380"
     CDS             3337..5556
                     /locus_tag="VFJ72_15380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7480885.1"
                     /translation="MTGEGGRGMSARERSGEHPSPPEDKEHRSIFENSLEGPLKDVTE
                     RKEIAEALRESNERITNILESVPDAFYALDREWRFTYLNEHAARDLKRSREELLGKNV
                     WEEFPGIVNSPFYQEYREALASGCPAHFEAYYPPLGRWFETYIHPSEPGLAVYFRDIT
                     ERKRAEGELRGALKELSDLKFALDESAIVAFTDQRGRITYVNDTFCEISKYSREELIG
                     QDHRIINSGFHPKGFIRNLWRTIAQGRVWRGELRNRAKDGSIYWVDTTIVPFLNERGK
                     PYQYVAIRHDVTDRKEAEAALREIREVERDRIARDLHDVVLQDLSYALQRLEDSRTSP
                     DGGRAAGMEDVEGVEEAVAALRRSTQGLRGAVYDLSQEASGGSFTRSLKTLAELQKRM
                     APECEVNLDVEEDFPEKLPQELEKEALLIVKEALTNVRRHSGAGRVAVTAGASAKKMW
                     VEVSDDGRGFQPDVDTGMGTRGMRQRARSLDGSLKITSTPGEGTRVRFEASLESGEYG
                     SGDIVEETRVLLVDDHASFRQGVAAALEREPGLAAIEQAGSLAEAREMLEGVEVGIFD
                     LNLPDGYGGELIKELRSANPRAAALVLSASLDRAEMARAVEYGAAGVLHKTAAMDEVV
                     DAIRRLKAGETLVPLEEVVELLRFASARKDQDYEAGQAIAQLTGREREVLEALAEGLD
                     AEEIARRLHISAKTERNHVARILAKLGVHSRLQALVFAARHGVVEVGRNSGTDPGNG"
     gene            complement(5668..5928)
                     /locus_tag="VFJ72_15385"
     CDS             complement(5668..5928)
                     /locus_tag="VFJ72_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480886.1"
                     /translation="MAGLEREMRDYEAGFMDADDAIRLFQKLVDTGIAWRLDERYQRT
                     ARALLEAGMIFPARGEAPLEAPEETKAFEDPAEWLAFAEATR"
     gene            complement(5948..6178)
                     /locus_tag="VFJ72_15390"
     CDS             complement(5948..6178)
                     /locus_tag="VFJ72_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480887.1"
                     /translation="MNITRQRARGHYEILETPYAKDYIWVTESEDTEVEVAEAEVAEE
                     LLRPWHVEYEEWVREQQVHHEQQQWIEIQALG"
     gene            complement(6429..>8005)
                     /locus_tag="VFJ72_15395"
     CDS             complement(6429..>8005)
                     /locus_tag="VFJ72_15395"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET7480888.1"
                     /translation="SQFSYNRAMGLVASSSAALVTLLSAHQSIGVPQPLKLFGTGEQK
                     RKWLPRLARGAVSAFALTEPEVGSDPARMKTYATPTESGGAYIINGEKLWCTNGPIAE
                     LVVVMARTPSRDGRRGITAFVVESDTPGFEVAHRSEFMGLRGIENGVLRFTNMRVPKE
                     NIIWGEGLGLKLALTTLNTGRLTIPAATAASAKWCLGVARRWANERRQWGAPIGKHEA
                     IAQKLADIAATTFAMEAIADLTALLADSGEVDIRIEAALAKLWNTEAAWRVADETMQI
                     RGGRGYETAESLERRGELPVPMERVMRDLRINTIFEGSSEIMRLFIAREAVDEHLKVA
                     GGLISSDLSTKDKLSAFPKIASYYASWYPRQWAALKTPRYEEFGELARHLRFIERSSR
                     RLARSTFHAMVRFGAKLEKKQAVLFRMVDIGAELFAMSAVCVRARWLLRQNPQDRSPI
                     RMADLFCRQSRRRVNDLFAALFRNDDRYAYEVSREVLAGEHDWIEAGIIGLEAPEGSM
                     TPVTHLPVEDGAGSAR"
BASE COUNT         1509 a         2616 c         2512 g         1368 t
ORIGIN      
        1 gctctccctg cacctcaaga gcctggcgat gtgcatggat ctcgggatca agaacctgga
       61 ggccaaccag ctcatcaact gcggaaggct gtacctgagc ctcggtgccc cggaagacgc
      121 cctggagcac ttccggcacg cagcccgcct cggccgggaa acggggtacg cccgggacga
      181 agggtactcg ctcatgggcg tcggcgtctg cctggagcac actggcagac ccgccagcgc
      241 cgcgaacgcc taccggcagg cgatcaaact tctggaaaca agctacgagc tgtccgaaat
      301 gccggaagaa ttgtccggga aagccgatgc cctggtcctc atggccgccg tgctcgaccg
      361 ctcgctcggc cgcccggtgg atgccctcga cgcttacgaa gaggccgccg agatttacag
      421 ccggctgggt gatccgctca agctacgcag tctccttgtg aggcgcgccg gtttgcgctg
      481 gcgtacaggg aacttcgaag gctccgcccg cgactacgag gaagcgctgg agcgggcgcg
      541 cggccaggga gagccggcgc gggaggctgc ggcgctcgcc agcctcagcg tggtctacag
      601 ggacctggga cgcctccagg agtcgctccg atccggccgg gccgccctga agttaatgag
      661 agacctggaa gatccccagg ccgaagccta cgtcttgagc agcctggccg agagccacga
      721 acagctcggg cattacccga gcgccctctc gtgcctcaag cgctcgttac gcctgcgccg
      781 gaagatcggg gacagagagg gcgaggtcgg ggctcttcac gacctggcaa ggatctacga
      841 gaccctggga gacctggatc gctccagtga agcttcggat gaagccatat cgaaaagaga
      901 agcattggaa gatggaccaa aagagggatt gaaagagggt cgtgaagtca tcgctaccgg
      961 ggaagggaga aactagatgc cgaaatacat catcgtccgg acggtcgggc agatctccga
     1021 ggaggagata gaagccgcgt ccatgaggtc catagaagcc ctggaccaga tgccgggggt
     1081 gcgttggatc cgctcctact actccgcgga ggaagggaag atctactgcg agtacgaggc
     1141 cccgagcgtg gagctgatct acgagcacgc tcagatggcg ggattaccgg tcgaccggtg
     1201 ttcggtggtg caggagctcg agccctcgat gttccgttag gtgtctcgga ttcgaagcga
     1261 tcactgctcc ccggatacta gcgcatctcc ctgtctgcca tcttgccgcg cctcttcacc
     1321 attgtctggt gcggatagat caggaccgga ccgcgagcca ccctgagcag gtttgtcgag
     1381 acactgccga gtctcgtacg acgggttacg ccaaacccgc ggcttcctac cgcgagcaaa
     1441 gtccttgatt cttcctcttc ttcggcaacc tgtagtaagg agagtgtggg gtcgctcccg
     1501 ataaccctgg cctcaggtcg cttgttcgag gtcttcccaa ggtcctccgc ccggtccttg
     1561 aggttctctt cgactctaag cgccactcta tcgagcctgg cacgatcctc ggcgctccag
     1621 ccacccgccg gttccggcgg attctggtgg gctcgcacca gcacacaacg agcatcgaac
     1681 aacccgccta tgcttgccgc gaactcggcc gcgctctggg catcctccga gccatcgtgc
     1741 cccgcgacga cacgatccgg cggccaccct ccatctacgc cgcgcaccac gagtacggga
     1801 caacggatgt ggtgggccac gccctccggg acgctgccga ccagcgcccg acggagccgt
     1861 ccccggccac ggctacccat caccaggaga tcgggcatag tctcctcgca tagatcgaga
     1921 atggcatcta ttggagaact cttgaggaga tgcgccgacg cgaccgtgcc tccgagagcc
     1981 tcgatcgcat cgacctgcga gccgagcacg cgccgggctt ccctttcgta gatttgcgcg
     2041 tagtcatccc aggccgcgtc ctgggaattg gagtacaggg gcacgacccg ccaggcgtgt
     2101 acgacgctta actctgcacc ggtcctctcc gcgaggtcta cggccgcccg catcgccaaa
     2161 cgggcatcct cggatccatc cgtggccagc gtgatgttat cgatcatgct cccagcctaa
     2221 ccagtaccat tccggctcgc tcatgccgac cctcggatac tattcggaca ggagacccag
     2281 cttgcgggcc ttcgccaggg cttcgagctg cgagtgcgcc cccagggcct gcaataccga
     2341 gcgcacgtga ttgcggaccg tggcctgcga caggtagagg accgagcaca tctccttgac
     2401 cgacttgccc tcggccagca gtcccagcac ctcggattgc cggggtgaga gcgccggcac
     2461 gtctctcgga tcgggaacag gaacatccct tgtggaggag agccgtatca ggccgcgggc
     2521 catctccagg gtctcgtgca tcttctgcga gtcgcgcatg aggtggatca ggtacggtcc
     2581 cgcatccgac tccacgctca ggacgctgac gttgacccat cgtttgttgc cccaccgggt
     2641 cgagacgcgc atatcgtagg agggcggcgc ctctccggcc ctcacgagcc tcatgacgga
     2701 acacctggaa ccacagaacg aaccgctgcc ctcgcactcg gcggacagcg ccgcatggca
     2761 ggtcccgcca gcaacctcct cggccatcag tcctgtcagg gactccatct gggcatccca
     2821 gtgaactata cggtactccg ggtccaccac gaaaaccgcg tccgccgtgc gcgccccgat
     2881 cagcgcctcg accgcgccgg ggagtctcgc gggatcttca gcagcgcccc gctcttcttt
     2941 ctcggtcatc gtggtcatcg cttgtccctc tcctttctga tgccctttac gagcgaatgc
     3001 gctacccggc accgtgccgt agtcctcctg ggtcttgtgc cggaccttta cagtagcttc
     3061 catcaccaca acctctcctc ctccgcggct tacgtaccga atgatcgccc ggagagcggc
     3121 tggctgtcgt gagcaaagct gctggtcttt acctgggcaa cacgcaccgc cggcacgtgg
     3181 gcaattttgc tcacgctgga tcttcggtgg tatcctgaga tgagtggaga gcgacctgaa
     3241 gcgacaccct atcggggctt caatgcaatc atcggggtcg cgaccgttgc cggtttgcgg
     3301 ccgtccatca cgccggccag acaggaggcc cctgacgtga caggagaagg cggtcgcggg
     3361 atgagcgcgc gcgagcggtc cggagagcat ccgagtccgc cagaggataa agaacaccgc
     3421 tccatcttcg agaactcgtt agagggcccg ctgaaagacg tgacggagcg caaggagatc
     3481 gcagaagcgc tgcgagagtc gaacgagcgg atcacgaaca tcctggagag cgtcccagac
     3541 gccttctacg ccctggaccg cgagtggcgg ttcacctacc tcaacgagca tgcggcacgc
     3601 gacctcaaga gatcgcgcga ggaacttctg ggcaagaacg tgtgggaaga gttcccgggg
     3661 atcgtaaata gtccgttcta ccaagagtac cgcgaggcgc tcgcgtcggg atgcccggcc
     3721 cacttcgagg cttactatcc gccgctcggc aggtggttcg agacctacat ccacccttcg
     3781 gagccaggac tggcggtcta cttcagggac atcaccgaga ggaagagagc cgaaggggaa
     3841 ctgcgcggag cgctcaagga gttatccgac ctcaagttcg ccctcgacga atcggccatc
     3901 gtcgccttca ccgaccagcg cggcaggatc acctacgtca acgacacgtt ctgcgagatc
     3961 tccaaatact cgcgagagga gttgatcggc caggaccacc gcatcatcaa ctccggcttc
     4021 catccgaaag ggttcatccg caacctgtgg cggaccatcg cccaggggcg cgtctggcgc
     4081 ggggagttga ggaaccgggc gaaggacggt tcgatctact gggtggacac gacgatcgtg
     4141 cctttcttga acgagcgcgg caagccctac cagtacgttg ccatccgtca cgacgtaaca
     4201 gaccgaaagg aagccgaggc ggcgctgcgc gagatccggg aggtagagcg cgaccgaata
     4261 gccagagacc tgcacgacgt ggtgctccag gacctctcct acgccctgca gaggctggaa
     4321 gattcccgaa cctcgccaga cggaggccgg gccgccggta tggaagatgt ggaaggcgtg
     4381 gaagaagccg tggcggcgct ccggcgctcc acacagggtc tgcgcggcgc cgtatacgac
     4441 ctgagccagg aggcgagcgg cggttctttc acccgttccc tgaagacgct ggccgaactg
     4501 caaaaacgca tggctccgga atgtgaagtg aacctcgacg tggaggaaga cttccccgaa
     4561 aagctcccgc aagagctgga gaaggaagct ctgctcatcg tcaaggaagc cctgaccaac
     4621 gtgagacgcc actcgggagc cggccgagtg gcggtaaccg cgggcgcctc cgcgaaaaag
     4681 atgtgggtcg aggtgtcgga cgacggacgg gggttccagc cggacgtgga taccggaatg
     4741 ggcacgaggg gtatgcgcca gcgggcgcgt tcgctggacg gaagcctgaa gataacgagc
     4801 actccagggg aaggcacgcg agtgcggttc gaagcgtctc tggagagcgg cgagtacggg
     4861 agcggggaca tcgtagagga gacgcgcgtc ctgctggtag acgatcacgc ctccttccgg
     4921 cagggtgtcg cggcggctct ggagagggaa ccgggactcg ccgccataga gcaggccggg
     4981 tcgctcgccg aggccaggga gatgctcgaa ggggtcgagg taggtatctt cgacctgaac
     5041 ctccccgatg gctacggcgg ggagttgatc aaagagcttc gctcggcaaa tccgcgcgcg
     5101 gccgctctgg tcctgagcgc tagtttggac cgggcggaga tggcccgggc ggtggaatac
     5161 ggggcggccg gggtcctgca caagacggcc gcaatggacg aggtcgtgga cgctatacgg
     5221 cgtctgaagg cgggagagac gctcgtgccc ctggaagagg tcgtggagtt actccggttc
     5281 gcgagcgcgc gaaaagacca ggactacgaa gccgggcagg ccatcgcgca actcaccggt
     5341 cgagagaggg aggtcctgga ggcgctggcg gagggcctcg acgccgaaga gatagccaga
     5401 cggctgcaca taagcgccaa gacggagcgc aaccacgtag ccaggatact cgcgaagctg
     5461 ggagttcact cccgcctgca ggccctcgtt ttcgccgccc ggcacggcgt tgtcgaagtc
     5521 ggccgcaact ccggtacaga tcccgggaac gggtaacacc cacggctacc atacacaagc
     5581 cccgcttcag agaagccccc gcgccaatcc aggactcttc tggaaagaac ccgggaaatg
     5641 tggcgtctag ctacgtcccg agcgctgtca gcgggtagct tccgcgaacg ccagccactc
     5701 cgccgggtcc tcgaaggctt tcgtctcctc cggggcctcc agaggcgcct ccccgcgagc
     5761 ggggaagatc atacccgcct caagaagggc ccgagcggtc ctctggtagc gctcgtccag
     5821 acgccatgcg atccccgtgt ccacaagctt ctggaagagc cttatcgcgt cgtccgcgtc
     5881 catgaagccg gcctcatagt ccctcatctc tcgctctagc ccggccatga cccgcacctc
     5941 ctctatatta gccgagggcc tgaatctcga tccactgctg ctgctcgtga tgaacttgct
     6001 gctccctgac ccattcctcg tattccacgt gccagggccg caggagttcc tcggcgacct
     6061 cagcctccgc aacctccact tcagtgtcct ccgattccgt cacccagatg tagtccttgg
     6121 cgtacggggt ctccaggatc tcgtagtgcc cccgcgctct ttgtctggtt atgttcatcc
     6181 ggacactcct ttcacgctcc cgcggtcgca ccggtcatac tcccggcttc tctcccgtct
     6241 gtgaacccct ccaggcttct ttcggggtcc ctctaaagga attgtgttcc ctgcgtggtc
     6301 atccgccatg atcaatagag actcattgtg tgatcaaatt tgatcaagcg ttcctcccgt
     6361 ggcggctcct tgtggacttg aagctccacg aataacagcc atcatcccta aaaggctcgc
     6421 gtcaacgctc aacgagcgga tccagcacca tcctctaccg gaagatgggt gaccggggtc
     6481 atggagccct ccggcgcttc tagcccgata atgcccgcct cgatccagtc gtgctctccg
     6541 gcgagcacct cgcgggagac ctcgtaggcg tagcggtcgt cgtttcggaa cagggccgcg
     6601 aagaggtcat tcacccggcg gcgagactgg cggcagaaga ggtccgccat cctgatcggg
     6661 gatctatcct gcgggttctg acgcagcagc cagcgcgccc ggacacacac ggcgctcatg
     6721 gcgaacaact ccgcccctat atccaccatg cggaagagga cggcctgctt cttctccagc
     6781 ttcgcgccga agcgcaccat ggcgtggaac gtggagcggg cgagcctgcg cgagctacgc
     6841 tcgataaagc ggaggtgacg cgccagctcg ccgaactcct cgtagcgggg cgtcttcagg
     6901 gcggcccact ggcgcgggta ccaggaggcg taatacgagg cgatcttcgg gaaggccgag
     6961 agcttatcct tcgtagagag gtcggaggag atcaagccgc ccgcgacctt caggtgctcg
     7021 tcaacggcct cgcgggcgat aaagaggcgc atgatctcgc tggagccctc gaagatggtg
     7081 ttgatccgca gatcccgcat cacacgctcc atcggcaccg ggagctcacc gcgccgctct
     7141 agagactcgg cggtctcgta gcccctgccg ccccggatct gcatcgtttc gtccgcgacc
     7201 cgccaggccg cctcggtgtt ccagagcttc gccagcgccg cctcgatcct tatgtccacc
     7261 tcgcctgagt ccgccaacag ggccgtgagg tccgcgatag cctccatcgc gaaggtggtc
     7321 gccgctatat cggcgagctt ctgggctatg gcctcgtgct tcccgatggg agcgccccac
     7381 tgccgacgct cattcgccca ccggcgcgcc acgcccaggc accacttcgc gctcgcggcc
     7441 gtcgcggcgg ggatagtcag ccggccggtg ttcagggtgg tgagcgccag cttcagaccc
     7501 agcccctcgc cccagatgat gttctccttc gggacgcgca tattggtgaa gcgcaggaca
     7561 ccgttctcga tcccgcgcag ccccatgaac tccgagcggt gcgcgacctc gaagcccggc
     7621 gtgtccgact ccaccacgaa cgccgttatt ccccgccgcc cgtcgcggga tggcgtgcgg
     7681 gccatcacga ccacaagctc ggctatcggg ccgttggtgc accacagctt ctcgccgttg
     7741 atgatgtacg cgccgccgct ctcggtgggc gtggcgtaag tcttcatccg ggccgggtcg
     7801 gagcccacct ccggctcggt gagagcgaag gcgctgacgg ccccacgggc gagccgggga
     7861 agccacttcc gcttctgctc tccggtcccg aagagcttca aaggctgcgg cacgccgatg
     7921 gactggtgcg ccgagagcag ggtgacgagc gccgccgatg aggaagctac cagccccatc
     7981 gcccggttgt agctgaactg ggaga
//
LOCUS       DASSMB010000271        19311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1357013, whole genome shotgun sequence.
ACCESSION   DASSMB010000271 DASSMB010000000
VERSION     DASSMB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 19311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19311
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357013"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /gene="larE"
                     /locus_tag="VFJ72_15400"
     CDS             <1..664
                     /gene="larE"
                     /locus_tag="VFJ72_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET7480889.1"
                     /translation="SELDLAAEFARSLGVEHLVVNTRELDDPNYASNPTNRCYFCKST
                     LYSNLGELAVARGYGCVVDGANKDDEGDYRPGRKAAKELNVVSPLSLAGVTKDEVRKL
                     AKYLGLPSWGKPALACLSSRFPYGQEITPEKLAQVARAEEFMRREGFQQVRVRHHGEI
                     ARLEVGSEELERAFAEREKITAELKDAGFLYVTLDLAGYKSGSLNAALGKPGKKSLPV
                     IG"
     gene            672..1241
                     /locus_tag="VFJ72_15405"
     CDS             672..1241
                     /locus_tag="VFJ72_15405"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HET7480890.1"
                     /translation="MAESEPLLWVTLAVMVVGLAGSVLPGLPGVPLIFLSALVYSIFT
                     DFEYVGILILALLFVLAALAFVADFFATSYGARRFGASNWGTVGGAVGGIVGALAGLL
                     FLGIGSLFGLILGTIGGVFVGEYLRRREQSGTSGERPRVERARSSRGDWRRASRAAGG
                     VLVGYLVSTITQGILGIVSIVVFVIALIY"
     gene            complement(1251..2150)
                     /locus_tag="VFJ72_15410"
     CDS             complement(1251..2150)
                     /locus_tag="VFJ72_15410"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HET7480891.1"
                     /translation="MDNRLINVEWRPDLERPVLIAGFTGWNDAAEAASVAVDTLSGSW
                     EAKRFGAFDSEEFFDFQVTRPHVSLIEGVTRKIEWPENELRATTRSLREIGDRGAVLL
                     SGPEPNFRWRTFSRVLAELAKELNVELVVTLGALLADVPHSRPVAVSANSQDPALIEN
                     LGLSSSRYEGPTGITGVLHSLCADHGVPSVSFWASVPHYLPSVPSAPAALALLASLSD
                     LLGIQADTSHLERTAGDYQEQVSAAVAQDTDLASYVQMLEERFDTAAAAGGIRDLPSG
                     DELAQELEGFLREQRREDGEDQI"
     gene            complement(2209..3315)
                     /locus_tag="VFJ72_15415"
     CDS             complement(2209..3315)
                     /locus_tag="VFJ72_15415"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7480892.1"
                     /translation="MAVAVALILLTVFAGKAWSAANLPTGFTQTRVATGLDNPTAMAF
                     APDGRIFVNQQGGKLRVIKNGTLLAAPAIDLTSRIDARGERGLLGVAFDPGFSSNHWI
                     YLYYTRKAAGSVPAHNRVARFRVVGDRLVASSQKVILDLNNLSSATNHNGGAIHFGSD
                     RKLYVAVGENANGDNSQSFKNLLGKMLRINKDGTMPRDNPFYKNKNVTGKNKAIWARG
                     LRNPYSFAVQPGTGRIYINDVGAQTWEEINAGRKGANYGWPIKEGPESAPKYTPPIFA
                     YRHGNSATTGCAITGGAFYNTSIVRFPSSFVGDYFFADFCNGWIRRYDPATDRAAAFA
                     TGIQNPVDLRVNNGYLYYLERGTGSLYQVDHPGA"
     gene            complement(3512..4072)
                     /gene="aat"
                     /locus_tag="VFJ72_15420"
     CDS             complement(3512..4072)
                     /gene="aat"
                     /locus_tag="VFJ72_15420"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HET7480893.1"
                     /translation="MEILEACYRAGAFPMADGYGRIQFYRSDPRSILELDSLHVSKSL
                     ARVIRKGTYEVYVDRDFGGVIRACARREETWIDAEIIAGFMRLHHAGRAHSVEAYREG
                     ELVGGLYGVALGGAFMGESMFSRMTDASKVCLVHLVERLKERGYTLLDCQIQNDHLAR
                     MGAVEIPEREYLRRLERALRLERNFV"
     gene            complement(4137..5078)
                     /locus_tag="VFJ72_15425"
     CDS             complement(4137..5078)
                     /locus_tag="VFJ72_15425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7480894.1"
                     /translation="MNYTELDWREAIILGAALRDGLLNAVANRLRSVREVAEELGMDA
                     RAVYVVLSALAELGLLDEEGGFRLREEHRRTLLDKEYSGYVGESVIHRFELMQSWGRL
                     PEVLRSGEPVEDRTLPDFGGTATFIQAMRRGARPGAEAVARTVLPRLPEGARILDVGG
                     GPGTNAEAFARGGARVTVFDRPEVIGQMRDILGRAGIETAAGDMNESLPEGPFDAIYF
                     GNTSHMYGPDENRALFARMRGSLAPGGLLVIREYLRGMSEDAALFAVNMLVLTARGGT
                     YTSAEYEEWLRAAGFEEVEILPVPGRGTHFVLARSPR"
     regulatory      5213..5284
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            5385..6116
                     /locus_tag="VFJ72_15430"
     CDS             5385..6116
                     /locus_tag="VFJ72_15430"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HET7480895.1"
                     /translation="MSTQERQQSPVSGSYPAVAQATLRLRSEPECERVIGLAHRRGIA
                     STMAYRGPEEDDSPIFVLVIDEVGILESFLPELQESVPGARISVIREEVAHVSPPDFL
                     RGGAIRNKPFRTEAGHLGWVFAGGALGAGARILTETGARYISPVHETFPWGTILVNAS
                     GSFAIAILGTLIAERFIGERERLFWVLGFLGSFTTFSSFVLQTTESWGDSAMLGTLYG
                     GGSIFLGLGAALAGIWLTRRTLRWE"
     gene            6107..6475
                     /locus_tag="VFJ72_15435"
     CDS             6107..6475
                     /locus_tag="VFJ72_15435"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HET7480896.1"
                     /translation="MGVIEVLVTLIGAGAGAVLRYVFGGWVNHRAGAGFPWGTLAVNT
                     VGCLALGLLEGIAAGDGFLLFVLGSGVIAGFTTFSTLMLETLNLSLAKEHDRALSNVL
                     GSVVLGIPALLAGLYLGGLV"
     gene            6472..6831
                     /locus_tag="VFJ72_15440"
     CDS             6472..6831
                     /locus_tag="VFJ72_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7480897.1"
                     /translation="MKPDDLRPAKRLRVFVGEDERYRGRPLFEAIVLAARDNGLAGAT
                     VFKGFMGYAPHADIAAAGILRLAENLPIVVDIVDDEERIRAFLPFLRQAVKTGLVICS
                     DVESEQVVPDVEGEDRR"
     gene            6828..7151
                     /locus_tag="VFJ72_15445"
     CDS             6828..7151
                     /locus_tag="VFJ72_15445"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7480898.1"
                     /translation="MTFLPATRARIYLRDSAEFEGRPAHALVFSAFRDAGVEDVSVHH
                     GVMGYDPASGLLSSRPLRFHADLPVVVEAVGSRERIEGALPRVREVLSRGLITLADVE
                     LYSAG"
     gene            complement(7136..8155)
                     /locus_tag="VFJ72_15450"
     CDS             complement(7136..8155)
                     /locus_tag="VFJ72_15450"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET7480899.1"
                     /translation="MGVPGPPPTDELSADAPLVAVRRGPLVESYHRGRIVFCGPDGEF
                     LDAVGAPDGYAYVRSSAKPFQALPLILSGAADACGLTDEDLAVACASHNAEERHLGAV
                     RSILRKAGLSESDLQSGAHPPMYAPAAAALARRGEEPRGIHGNCSGKHAGMLAVCAHQ
                     GWETETYRKPDHPLQRWISDLVSKVCAVEQDALVVSGDGCGLPAFAMPLRSLATGFAR
                     LATGRCLPDDLAQAALRIRDAMRAHPFMVAGTGRLDTEVMEATDLVTKMGAEGVLGVG
                     SLDGWGMALKVSDGAGRAVRPAALAALSRRGVEASVSASAPVNDLHGVQVGEITSLWT
                     SQPAE"
     gene            complement(8158..8478)
                     /locus_tag="VFJ72_15455"
     CDS             complement(8158..8478)
                     /locus_tag="VFJ72_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480900.1"
                     /translation="MRRAKRESSAADLRLGHLLRVNGDLEIAILSMWMRSPRAKVMIG
                     MAQASLRGEGPGGTDADLLEKLRPLVIEAREFYADDDFPAAMARMRVAHDLVGLRIIS
                     VGKG"
     gene            complement(8475..9308)
                     /locus_tag="VFJ72_15460"
     CDS             complement(8475..9308)
                     /locus_tag="VFJ72_15460"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7480901.1"
                     /translation="MSTETEREHNLDALTSGAMSGIWEWVRSKIPADLEIFDAHAHIG
                     ADVDTRTMTADGLRDRMRAAGVGRSIVFALNDPNARDDFSGPNDVVWNAYEEYPNLYV
                     PFFRLNPHRDYEKEFERCVELGFQGLKLHPVSQVFELDEPRVVKLLGMAAEADLPVLI
                     HTGFAMQRIVEPLIPTVEAHPELRLILGHSAMVEILDAVRAFEDHPNVLFETSVVRAK
                     DLYVLFSILDPARISYGSDIPYGDYPSTLHATLAAADAAGVPDSALPGLLSGNIRRWF
                     P"
     gene            complement(9412..9795)
                     /locus_tag="VFJ72_15465"
     CDS             complement(9412..9795)
                     /locus_tag="VFJ72_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480902.1"
                     /translation="MRRAEYEQRVLQELRRILLGPGGEPKRFKDRGYRKIQVEDVRLD
                     ESKPEHELIILFRDLTRPECLFGFNMEAVERGQLIGPDDAHTFYTTLEDAAQGWTIVV
                     WANFQERIIGEPLPTDCLPDETTWV"
     gene            complement(9782..11053)
                     /locus_tag="VFJ72_15470"
     CDS             complement(9782..11053)
                     /locus_tag="VFJ72_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7480903.1"
                     /translation="MLVGLNAFFVAAEFALVRVRESRIVQLEQEGSVRAGGVRAALRD
                     LDAYLSICQVGITVASLALGWVGEPAVSHLIRPVLEAVGIGSERIIEIIAVALGFAAI
                     TYAHLVFGEQAPKYFSIQKAERVAMFIIRPLKFFMLVLRPVVWLVNASTNFALRPWGI
                     RLGEEMEAHSEEELRIMISSSTASGVLDPEERDYLNNVFDFGDTVAREIMVPRPDIEA
                     LPYDAPLPELAEKAAFGRYTRYPVYEEDLDHVLGAVHVKDLFRAAREEPENFDIRPLI
                     RECLVIPENKRIDGILKEFQQRKLQMAIVIDEWGSVEGLITIEDIVEELVGEIQDEFD
                     EGEAAIEKIGDDLYAIDGRIPITDVNEHFDLDLPHEDFDTIGGYILGSLGRPPEPGDT
                     VEAEGVALNVKSVDGPRVSMLTLRRDADASG"
     gene            11307..12446
                     /locus_tag="VFJ72_15475"
     CDS             11307..12446
                     /locus_tag="VFJ72_15475"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HET7480904.1"
                     /translation="MLAAAFGVLLVAILALAAWSLTGSRPETASAEPGTYAQVSTPRT
                     GEKDDLTTWGDSADPVKAIYLTAYNAGSANLEAYLKLLDRTELNAVVVDVKDVTGEVM
                     YPSKVPLANEIGATRQVLDLKSLTKQLKERDVYAIARVAVFEDDILPRQRPDLAVTDS
                     STGAPWLNDAGQTWANQYNKEVWKYNVAVAKEAARAGFDEIQFDYIRFPSDGPMETID
                     YGKETYPKQQEALAAFLKYADSELHPMGVRVASDVFGLAATEDGAGVGQYIDTLAPYL
                     DVVCPMAYPSHYPPGSYGYDDPNSEPYKVIEHTMTDFEKKTKDANPGIEIRPWLQDFT
                     LGTPPYGPEEVRAQMDAVYDSGATGWLLWNADNDYTEMALKASSR"
     gene            complement(12509..13018)
                     /locus_tag="VFJ72_15480"
     CDS             complement(12509..13018)
                     /locus_tag="VFJ72_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7480905.1"
                     /translation="METNRGSEGILSEEMAGKREEIVELLKKAYFMELETVINYVTNS
                     TNPDGVRAQEIKESLEEDIQEELQHAQDFAARIKELYGVVPGSAEFVAEQSFLQPPED
                     QVDVVHVIKGVIEAETGAIQHYTRLIEATEEVDPVTQDMVIAILRDEQGHRKLFEGFL
                     REYEAEGLA"
     gene            complement(13140..14891)
                     /gene="glpD"
                     /locus_tag="VFJ72_15485"
     CDS             complement(13140..14891)
                     /gene="glpD"
                     /locus_tag="VFJ72_15485"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HET7480906.1"
                     /translation="MDRQVPEDIRDYPFDLIVVGAGINGAGIARDAAMRGLKVLLLDK
                     GDIASGTTQWATRLIHGGLRYLEHYEVHLVRESLAEREKLLHIAPHLVKPLKFVVPIY
                     ERSKRGPGLIRLGMMGYDALSFDKSVPNHKMLSRDEALEQYPGINPDGLRGAATYYDG
                     QVEYAERVAVENAISAREHGATVLTYAEVKRLLFEDGGSRVAGVEFEDRLGGGTHTAH
                     APVTVNVAGPWVDRVLRGSGASGFAEAPENGAEEDSGRMIGGTKGSHIVVDAFPGAPR
                     DALYVEARRDGRPYFIVPWNGRYLIGTTDFRYRGDLDYVSADDDEIQYLIDETNAVVP
                     QAALTRESVLFTYSGVRPLPFKPEGSESSITRSHVVYDHATGASAAGGRLTVSGAGPK
                     ADGLISIVGGKLTTYRNLARQTVDVVYDKLHLHAPPCRTDKVPLPGGAAEDFDNFAAE
                     FKATSGLTDMLAERLLKLYGTRAPDVIAEARDDHSLKMPLSASATVETGIIGAEVLYA
                     FRREMAETLSDVLLRRTMVGMGPRVALDLDSAAAEVAVRYLGWSSERSKDEVRKYREY
                     VQRYRPKDFRKRKTVRV"
     gene            complement(14907..15299)
                     /gene="dhaM"
                     /locus_tag="VFJ72_15490"
     CDS             complement(14907..15299)
                     /gene="dhaM"
                     /locus_tag="VFJ72_15490"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HET7480907.1"
                     /translation="MVGLVLVSHSPRIADGTAELIGHMAGDVEVVAVGGDSDGGLGTD
                     PEGVQAAIEGLTAEEVLVFMDIGSAVLSAETVLEMLSSETREKVRLVDAPFVEGAFAA
                     GVMASTGADAGECVEAAMEARTESKLHD"
     gene            complement(15299..15925)
                     /gene="dhaL"
                     /locus_tag="VFJ72_15495"
     CDS             complement(15299..15925)
                     /gene="dhaL"
                     /locus_tag="VFJ72_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HET7480908.1"
                     /translation="MGATEATLNVIREMAAAMEEHRRYLTKLDSEIGDGDHGTNMHRG
                     FQAALERLDGAELNSPADVLKAVSMALISKVGGAAGPLYGTAFLRASTALGDKEEVSG
                     EDTAAALEAALGGIKQRGKAEVGDKTIVDALTPAVEAAKGASGGSAAEVFRAAADAAE
                     EGAESTVPMTAKRGRASYLGARAAGHRDPGATSTQFLLDAAARALESE"
     gene            complement(15928..17445)
                     /locus_tag="VFJ72_15500"
     CDS             complement(15928..17445)
                     /locus_tag="VFJ72_15500"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HET7480909.1"
                     /translation="MNGGSPASIVESFIRHAERAPERLCLRFEGEEWTYGSLRQRAQA
                     FASALRGRGLGPGDRVALFLENCPDLLAAYLGTHLSGGVVVPFNTQYRRTELRHIFED
                     SGARLCLTDAERRVELERVRKDLAHLETVVEVGEELEDFLGSGEERKFGMPDGEDLAV
                     VAYTSGTTGRSKGAMLLHRNLAANAAAVCEAWRWTEEDRLLLALPLFHTHGLMVGAHG
                     TFYMGASAELHRRFDAAKVYEALLEGWVTMFFGVPTMYTRLLREAEKRDERPAPLRLY
                     VSGSAPLSPQAFEEFERLFGERILERYGMTETIMNLTNPFDGERRPGTVGGPFPGQEA
                     RVVDLETRKLLPPGEDGEIEVRGPHVFAGYLNRPDATEESFYEDGWFRTGDLGSVSED
                     GYYTISGRAKELIISGGYNVYPREVEEVLERFPGVVEAAVVGLPDPEYGERVVAAVVR
                     DDPSLTAEKLTEFCREELAGYKKPREVAFVDELPRNALGKTLKHEVREKLMESEE"
     gene            complement(17446..18882)
                     /locus_tag="VFJ72_15505"
     CDS             complement(17446..18882)
                     /locus_tag="VFJ72_15505"
                     /inference="COORDINATES: protein motif:HMM:NF025065.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7480910.1"
                     /translation="MNAADRLPLADPTDLDSMREALAAADPEGALNPIEMRRIEIGPE
                     AIELLSEVVSEVARGRNVVLVCDSTPILRSGEDLKDWAERTLGEIFEVRRVVIGDDEH
                     ELHADEEALAEVEAAVEGADCVVAVGSGTITDLCKEATARKGDIPLVVVQTAASVNAF
                     AINMSVLLKSGVKRTGTSKWPDALLIDLPILAGAPRVMSLAGFGDLLATWTSPADWYL
                     AALVGMDDSYNPAPLNMVFDQAREVLDGAAAVRRHEPEALEKLARCLALGGMAAGVIG
                     KTAPLSGTEHLVSHLLDMNAGKRGLPFNFHGAQVGIATVPVAAAWEELFFRLDPAEID
                     IDACFPDPEAVEPMVRDAFSDLDPTGAIAEECWNDYALKLERWRTARPRFEEFFANWS
                     RLRNELREMVFSPEWLGKALQESGAPARFRDLDPPVPAETAFWALRNCHLMRNRFTVA
                     DLLFFLGWWDDAFVEGLLERARSVGGGL"
     gene            complement(18920..>19311)
                     /locus_tag="VFJ72_15510"
     CDS             complement(18920..>19311)
                     /locus_tag="VFJ72_15510"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7480911.1"
                     /translation="THSRFATTLACLGWEIPPVHYMLTTLGPDGRIPLAPYVLYGTEQ
                     LAGNAASALGESHNACLLANHGTIAVGENAEKAFARTVVLEEMAEIYYRTRLAGEPVL
                     LDPEQISEVAAKIQGYGQPTSVSESAE"
BASE COUNT         3173 a         6598 c         6152 g         3388 t
ORIGIN      
        1 ttcggagctg gatctggcgg cggagttcgc ccgttccctg ggcgtggagc acctggtggt
       61 caacacccgc gagttggacg acccgaacta cgccagcaac cccaccaacc gctgctactt
      121 ctgcaagagc acgctctact ccaacctcgg agagctggcg gtagcgcgcg gctatgggtg
      181 cgtcgtagac ggggcgaaca aggacgacga gggcgattac cgtccggggc gcaaggcggc
      241 gaaggagctg aacgtagtct cgccgctctc cctcgcggga gtgacgaagg atgaggtgcg
      301 gaagcttgcg aagtacctgg ggctgccgag ctggggcaag cccgctctgg cgtgcctgtc
      361 cagccggttc ccttacgggc aggagatcac gccggagaag ctcgcccagg tcgcgcgagc
      421 ggaggaattc atgcgccgcg agggcttcca gcaggtgcgg gtgcgtcacc acggcgagat
      481 agcccgcctg gaagtcggct cggaggagct ggagcgggcc ttcgccgagc gcgagaagat
      541 aaccgccgag ctaaaggacg ccggtttcct ttacgtcacg ctcgacctcg cgggatacaa
      601 gtccggtagc ctcaacgccg cgctcggcaa gcccggcaag aagagcctgc cggtgatcgg
      661 ctaggtccgt agtggctgag agcgagccgc tgctctgggt cacgctcgcc gtcatggtcg
      721 tcgggctggc cgggagcgtg ctgccgggcc ttcccggagt acccctgatc ttcctctccg
      781 ccctcgtcta ctcgatcttc acggacttcg agtacgtcgg tatcctgata ctcgcgctgc
      841 tcttcgtcct ggccgcgctc gccttcgtcg cggacttctt cgcgacctcc tacggggccc
      901 gcaggttcgg agcgagcaac tggggcacgg tcggcggagc cgtgggcggc atcgtcgggg
      961 cgctcgccgg gctgctgttc ctcggcatcg gttccctctt cggcctgatc ctgggcacca
     1021 tcggcggcgt cttcgtcggc gagtacctgc gccgcagaga gcagtccggt acgagcggtg
     1081 aacgaccgag ggtcgagagg gcgcggtcct cgcgaggaga ctggcggcgg gcgtcccgcg
     1141 cggcgggcgg cgtgctagtc ggctatctgg tctcgaccat cacccagggg atactgggga
     1201 tcgtcagcat cgtggtcttc gtgatcgccc tgatctacta gaaagacctg ctagatctgg
     1261 tcctcgccgt cctcccggcg ctgctcgcgc aggaagccct ctagctcctg ggcaagctcg
     1321 tcgccggagg gcaggtcccg tatcccgccc gcagccgccg cggtgtcgaa gcgctcttcg
     1381 agcatctgga cgtaggaggc gaggtccgtg tcctgggcga cggcggcgga gacctgctcc
     1441 tggtagtcgc cggcggtccg ctccaggtgc gaggtgtccg cctggatacc cagcaggtcg
     1501 gagaggctcg ccaggagagc gagagcggcc ggcgcggagg gcaccgaagg caaatagtgc
     1561 ggcaccgagg cccagaagct caccgagggc acgccatgat ccgcgcagag gctgtgcaac
     1621 acgccggtta tgccggtcgg gccctcgtag cgcgaggacg agaggccgag gttctcgatc
     1681 agggccgggt cctgcgagtt ggccgagacg gcgacgggcc gcgagtgggg cacgtcggcg
     1741 agcagggcgc ctaaagtgac cacaagttcc acgttcaact ccttcgccag ctccgcgagc
     1801 accctcgaaa aagtgcgcca ccggaagttc ggctccggcc cggagaggag cacggccccc
     1861 cggtccccga tctcgcgcag gctccgggtg gtagcgcgca actcgttctc gggccattcg
     1921 atcttccgcg tcacgccctc gatcaggctc acatgcgggc gcgtaacctg gaaatcgaag
     1981 aactcctccg agtcgaaagc gccaaacctc ttcgcttccc aggagccgga gagcgtatca
     2041 acagccacgc tcgccgcctc cgccgcgtcg ttccaacccg taaatcccgc gataagcacc
     2101 ggacgttcga ggtccgggcg ccactcgacg tttataagtc tgttatccat cccgcaagct
     2161 taccacgcgc cctgcccggc acgatgcaga cgcccacttc ggacacggct aggcccccgg
     2221 atggtccacc tggtagaggg aacccgtgcc gcgctccagg taatacaggt agccgttatt
     2281 cacccgcagg tccaccggat tctggatgcc ggtcgcgaat gccgccgctc tgtcggtagc
     2341 gggatcgtag cgccggatcc aaccgttaca aaaatccgcg aagaagtagt cgccaacgaa
     2401 gctggacggg aaccttacga tggaggtgtt gtagaaggct ccgccggtga tcgcgcatcc
     2461 cgtcgtcgcg gagttgccgt gccggtaggc gaagatggga ggagtatact tcggtgccga
     2521 ttcggggcct tccttgatcg gccatccgta gttggcgccc ttgcggccgg cgttgatctc
     2581 ctcccaggtc tgcgcgccca cgtcgttgat gtagatgcgc cccgtgcctg gctgcacggc
     2641 gaagctgtaa gggttgcgaa gcccgcgcgc ccagatcgcc ttattcttcc cggtgacgtt
     2701 cttattcttg tagaaagggt tgtccctcgg catggtgccg tccttgttga tcctcagcat
     2761 cttgccgagc aggttcttga agctctggga gttgtcaccg ttggcgttct cacccaccgc
     2821 gacgtacagc ttgcggtccg agccgaagtg aatggcgcca ccgttgtggt tcgtggcgct
     2881 actcaggttg ttgagatcga ggatcacctt ctgactgctc gcgaccagcc ggtcgcccac
     2941 caccctgaac cgcgctacgc ggttgtgcgc cggcacggag cccgcggcct tccgcgtgta
     3001 gtacaggtag atccagtggt tgctgctgaa gccgggatcg aacgcgaccc cgagcagccc
     3061 gcgttcaccc ctggcgtcta tgcgggaagt gaggtctatg gccggcgcag ccagcaacgt
     3121 cccgttcttg atgaccctca gctttcctcc ctgctggttg acgaagatcc tgccgtccgg
     3181 cgcgaaggcc atcgcggtcg ggttgtcgag ccccgtggct actctggtct gcgtgaaccc
     3241 ggtaggcagg ttggccgcgc tccacgcttt cccggcgaag accgtaagca atatcagcgc
     3301 tacggctacc gccacgccag cgcctatcgt caggccacgc ttgctggttt catgcccgaa
     3361 gaagtgaccg gggaaaggtg tccgggccga ccttatatcg cgttccaaag ctacctcagt
     3421 tcgtcacggg gctctaatcg ctggcatact acatcctgcc agctctcgcc gagtagttgg
     3481 cgacacaact tcacaattac tttagggctg ctcagacgaa atttcgttcg agccggagcg
     3541 cccgttccag acgccggagg tactctcgct cggggatctc gacggcgccc atgcgggcca
     3601 ggtggtcgtt ctgtatctgg cagtcgagta gcgtgtaccc ccgctccttg agccgctcta
     3661 cgagatgaac caggcagact ttcgaggcgt ccgtcatgcg gctgaacatg gactcaccca
     3721 tgaacgcccc gccgagggca accccataga gtccgccaac cagttccccc tcccggtacg
     3781 cctcgacgct gtgcgccctg cccgcgtggt gcagacgcat gaacccggca atgatctcgg
     3841 cgtctatcca ggtctcttcc cggcgggcgc aggcccggat cacgccgccg aagtcccggt
     3901 ctacgtacac ctcgtaagtc cccttgcgga tgacccgcgc gagcgatttc gagacgtgca
     3961 gcgaatccag ctccaggata gacctcgggt ccgagcggta gaactgtatc cggccatacc
     4021 cgtcggccat cgggaaagcg cccgcccggt agcaagcctc cagaatctcc accgtcaacc
     4081 tcacgccccg aataatagtg cggcctaccg ccggcgcacc agcacggacc ccatcctcac
     4141 ctcgggctgc gggcgagtac gaagtgcgta ccgcgtcccg gcacgggtag gatctcgacc
     4201 tcttcgaatc ccgccgcccg gagccactcc tcgtactctg ccgaggtgta ggtcccgccg
     4261 cgcgcggtca ggacgagcat gttgacggcg aagagcgccg cgtcctcgct catgccgcgt
     4321 aggtactccc ggatcaccag cagcccgccc ggagctaacg agccgcgcat cctggcgaag
     4381 agcgcgcggt tctcgtcggg tccatacata tgcgaggtgt tgccgaagta gatcgcatcg
     4441 aacggccctt ccggcaggct ctcgttcatg tctcccgccg cggtctcgat cccggcccgc
     4501 cccaggatgt cccgcatctg cccgatgacc tccggccggt cgaagaccgt cacccgcgcc
     4561 ccaccgcgcg cgaacgcctc cgcgttggtt cccggaccgc cgccgacatc gagtatccgc
     4621 gctccctccg gcagccgggg caggacggtc cgtgccacgg cctcggctcc agggcgcgct
     4681 ccgcggcgca tggcctggat gaacgtcgcc gtccccccga agtccgggag ggtccggtcc
     4741 tcgacgggct cgccggagcg tagtacctcc ggcagcctgc cccagctctg catgagctcg
     4801 aagcggtgga tcacgctctc ccccacgtag ccggagtatt ctttatccag caaagtccgc
     4861 cggtgctcct cccgcaaccg gaagccgccc tcctcatcca gcaagcccag ctccgcgagc
     4921 gccgagagaa ccacgtaaac cgcccgcgca tccatcccga gctcttccgc cacctctctg
     4981 accgagcgga gtctattagc gacagcgttg aggagaccgt ccctcagcgc cgcgccgagg
     5041 atgatcgcct cccgccaatc gagctccgtg tagttcatct cacccatcga agtccatgat
     5101 tgtatcctcc gatatccgaa cgcggtgcgg ggacagttcg aagatagttc cgggccgcgg
     5161 ctccgggata ctccttgatc aagctcgcca ctctttgtaa acttcttgta gacaactcgc
     5221 gggtgatgag gctcgccttt aaccgcgttc gttcgctacg ttcgctaatg gcttctacgc
     5281 gccgggccgc cggggtgcca acggggtacc ggaaggcccg ggtaggagct tttttcgtgt
     5341 ccggtccggc tggcgcgaga acacgaagga gggctggtag gaccatgagt acgcaggagc
     5401 ggcagcaaag tccggtatca ggctcttatc cggcggtggc tcaggccacg cttcgtctgc
     5461 gttcggagcc ggagtgtgag cgcgtgatcg gtctggccca tcgccggggg atcgcctcca
     5521 ccatggccta ccggggaccg gaagaagacg actccccgat cttcgtgctg gtaatagacg
     5581 aggttggcat cctcgaatcg ttcctgcccg agttgcagga gtcggtgccc ggtgcgcgca
     5641 tctccgtgat ccgcgaggag gtggcccacg tatcgccgcc ggacttcctg cgcgggggcg
     5701 ctatccgcaa caagcccttc cggacggagg ccggacatct cggctgggtc ttcgcgggcg
     5761 gcgccctggg ggccggggcc agaatactca cggaaacggg ggccaggtac attagcccgg
     5821 ttcacgagac gttcccctgg ggcactatac tggtcaacgc ctccgggtct ttcgcaatag
     5881 cgatccttgg gaccctgatc gccgagcggt tcatcgggga gcgcgagcgg ctgttctggg
     5941 tgctgggctt cctgggctcg ttcacgacgt tcagttcctt cgtacttcag acgaccgaga
     6001 gctggggaga ttccgcgatg ctcgggactc tctacggcgg tgggagcata ttcctcgggc
     6061 tgggagccgc gctggccggg atctggctga ccagaaggac gctccggtgg gagtgatcga
     6121 ggtgctggta accctgatcg gagccggagc gggcgcggtc ctgagatacg tcttcggagg
     6181 ctgggtcaac catcgggcag gagcgggctt tccgtggggc acgctggcgg tgaacaccgt
     6241 gggctgtctc gcgctgggcc ttctcgaagg gatcgccgcc ggagacggat tcctcctgtt
     6301 cgttctcggc agcggagtca tcgccggctt cacgacgttc tccacgctga tgctagagac
     6361 cctgaacctc tcgctggcga aggagcacga ccgggcgctc tccaacgtgc tcggcagcgt
     6421 ggtactcgga attccggcgc tcctggccgg actgtacctc ggagggctgg tgtgaagccg
     6481 gacgatctgc ggccggcgaa gaggctgcgg gtcttcgtgg gagaggacga gcgttaccgc
     6541 ggacgtccgc tcttcgaggc gatcgtgctc gccgccagag acaatgggtt ggcgggggcg
     6601 accgtcttca aggggttcat gggctacgct ccgcacgccg acatcgccgc ggcaggcatc
     6661 ctgcgtctgg cggagaacct gccgatcgtc gtggacatag tggatgacga ggagcgcatc
     6721 cgggcgtttc taccgtttct gcgccaggcg gtcaagaccg ggctcgtcat ctgctcggat
     6781 gtggagtctg agcaggtcgt tcccgatgtt gagggagagg ataggcgatg accttcctac
     6841 cggcgacgcg ggccaggatc taccttcgcg acagcgccga gttcgaggga cgcccggctc
     6901 acgccctggt cttctcggcg tttagagacg cgggtgtaga agacgtctcc gtacaccacg
     6961 gggtcatggg ctacgacccc gcttcgggac tactctcctc gcgccccctg cgcttccacg
     7021 cggacctgcc cgtggtagtc gaggctgtcg ggagcagaga acgaatcgag ggcgcactcc
     7081 ctcgcgtaag agaggtcctc agccgcggtc tcatcacgct cgcggacgtg gaactctact
     7141 cggcgggttg actggtccag agagaagtta tctcccccac ctggacaccg tggaggtcgt
     7201 tgaccggcgc ggacgccgaa acagacgctt ccacgccccg gcgcgagagc gcggcgagcg
     7261 cggccgggcg aacagccctc cccgcgccgt cggagacctt gagcgccatc ccccacccgt
     7321 ccaggctgcc gacgccgagc acgccctcgg cgcccatctt ggtgacgagg tctgtggcct
     7381 ccatgacctc cgtgtcgagt ctccccgtac cggcgaccat gaacggatga gcgcgcatcg
     7441 cgtcccggat gcggagcgcg gcttgcgcga ggtcgtccgg caggcatcta ccggtggcga
     7501 gccgcgcgaa gccagtcgcg aggctcctta gcggcatggc gaaggcgggt aggccgcagc
     7561 catcgccgct aacgacaagc gcatcctgct cgacggcgca tacctttgat acgaggtccg
     7621 agatccagcg ctgcagcgga tggtccggct ttctgtaggt ctccgtctcc cagccctggt
     7681 gcgcgcagac ggcgagcatc ccggcgtgct tgccggagca gttgccgtgg atgccgcggg
     7741 gctcctctcc ccggcgagcc aacgcggccg ccgccggagc gtacatcggc ggatgagccc
     7801 cgctctgaag gtcactctcc gaaagaccgg ccttgcggag gatcgagcgc accgcgccca
     7861 ggtgacgctc ctcggcgttg tgcgaggcgc aggcgacggc cagatcctca tccgtcagcc
     7921 cgcaggcgtc ggccgcgccg gagaggataa gcgggagcgc ctggaagggc ttcgccgagg
     7981 agcggacata ggcgtacccg tccggggcgc cgactgcgtc caggaactca ccatcaggtc
     8041 cgcagaagac tatacgtccg cgatggtagc tctctacgag aggaccgcgg cggacggcga
     8101 cgagcggcgc gtcggcggac aactcatccg tcggcggcgg gccgggaacc cccaccgcta
     8161 ccctttgccg acgctgatga tcctgagccc gaccaggtcg tgcgcgacgc gcatgcgggc
     8221 catagcggcc gggaaatcgt cgtccgcgta gaactcgcgc gcctcaatga ccagcggccg
     8281 caacttctcc aggaggtcgg cgtcagtccc gccgggcccc tcgccgcgca ggctcgcctg
     8341 ggccatcccg atcatcacct tcgcgcgcgg gctcctcatc cacatggaga gtatcgcgat
     8401 ctccaggtcc ccgttcacgc gcagcagatg cccgagacgc aggtcggcgg cggagctctc
     8461 ccgcttcgcc cgcctcacgg gaaccaccgt cggatgttcc ccgagagcaa ccctggcagc
     8521 gccgagtccg gcacccccgc ggcgtcggcg gcggcgagcg tggcatgcaa cgtcgaggga
     8581 taatcgccgt agggtatgtc cgaaccgtag cttatgcgcg ccgggtccag gatgctgaac
     8641 agcacgtaga ggtccttcgc ccgcaccacc gaggtctcga agagcacgtt gggatggtcc
     8701 tcgaaagccc ggacggcgtc caggatctcg accatggccg agtgtcccag tatgagccgc
     8761 aactccgggt gcgcctcgac ggtcggaatc agcggctcga cgatccgctg catggcgaac
     8821 ccggtgtgta tgagcaccgg cagatcagcc tccgccgcca taccgagcaa cttcacgacc
     8881 cgcggctcgt ccagctcgaa cacctgggag accgggtgca gctttagtcc ctggaagccc
     8941 agctccacgc accgctcgaa ctccttctca tagtcgcggt gcgggttcag ccggaagaac
     9001 ggcacgtaga ggttcggata ctcctcgtag gcgttccaga ccacgtcgtt cggccccgag
     9061 aagtcgtcgc gcgcgttcgg gtcgttcagc gcgaagacta tcgaccgtcc cacgcccgcc
     9121 gcccgcatcc ggtcgcgcag tccgtcggcg gtcatcgtgc gggtatccac gtccgctccg
     9181 atgtgggcgt gggcgtcgaa gatttccagg tctgcaggga tcttgctccg cacccactcc
     9241 cagatcccac tcatcgcccc gctggtcagg gcgtcgaggt tatgctctct ctcggtctcc
     9301 gtagacaatg gctcgcctct tagctaggtc tcaccagaca caacattcgt gcaatggtag
     9361 caggcgaggt atgacaccgc taagtccggc aagatctaca accagataac ctcagaccca
     9421 ggttgtctca tcaggtaaac agtctgtcgg aagtggctcg ccgatgatcc gttcctgaaa
     9481 gttggcccag acaacgattg tccatccctg ggcagcatct tccagagtgg tgtaaaacgt
     9541 gtgcgcgtca tcaggtccga ttagctgtcc ccgctctaca gcctccatgt tgaacccgaa
     9601 caggcactcg ggacgggtca gatcccggaa caagattatt agctcgtgct ccggcttcga
     9661 ctcgtcgagc cggacatcct caacctggat cttcctgtat cccctgtcct tgaacctctt
     9721 cggctctccg cccgggccga ggagtatcct gcggagttcc tgtaggaccc tctgctcgta
     9781 ctcagcccga cgcatcggcg tcgcggcgca gtgtgagcat ggagacgcga gggccgtcca
     9841 cgctcttgac gttgagggcg acgccttcgg cttcgacggt gtcgccgggc tcgggggggc
     9901 gtccgagaga gcccagtatg tagccgccga tggtgtcgaa gtcctcgtgc gggaggtcca
     9961 ggtcgaagtg ctcgttcacg tcggtgatgg ggatgcgtcc gtctatggcg tagaggtcgt
    10021 ccccgatctt ctctatggcc gcctcgccct cgtcgaactc atcctgtatc tccccgacaa
    10081 gctcctccac gatgtcctca atcgtgataa gcccctccac gctcccccac tcgtctatga
    10141 ctatcgccat ctggagcttg cgctgctgga actccttgag gatcccgtct atgcgcttgt
    10201 tctccgggat gacgagacac tcccttatga gcggccggat gtcgaagttc tccggctcct
    10261 ctctggccgc ccggaagagg tcctttacgt ggaccgcgcc gaggacgtgg tcgaggtcct
    10321 cctcgtagac cgggtagcgc gtgtagcgtc cgaaggccgc cttctccgcc agttccggca
    10381 gcggagcgtc gtagggcagc gcctcgatat ccgggcgggg aaccatgatc tcgcgggcca
    10441 ccgtgtcccc gaagtcgaag acgttgttca ggtagtcgcg ctcctccggg tcgagcaccc
    10501 ccgacgcggt agaggaggag atcatgatcc gaagctcctc ctcggagtgg gcctccatct
    10561 cctcgccgag ccggatgccc cacggcctga gcgcgaagtt cgtcgaggcg ttaacgagcc
    10621 acaccacggg acgcaacacc agcatgaaga acttgagggg gcggataatg aacatcgcta
    10681 ccctctccgc cttctggata gagaagtact ttggcgcctg ctctccgaag accagatgcg
    10741 cgtaggtgat ggcggcaaac cccagcgcga ccgcgatgat ctcgatgatc cgctcgctgc
    10801 cgatgcccac ggcctccagc accggacgta tcaggtgcga gaccgccggc tcgccgaccc
    10861 aaccaagagc cagcgacgcg accgtgatcc cgacctggca gatggagagg taggcgtcca
    10921 ggtccctgag agccgcccgc accccgccgg cccgcacgct tccctcctgc tctaactgca
    10981 ctatccgcga ctcgcgcacg cgcacaagag cgaactccgc cgccacgaag aaggcgttca
    11041 aaccgaccag caccagggcc gccgccagat ttaaccagga taagagaggc tcttgcacgt
    11101 ctacacgctc acgccgcggc cagaagtata cgaagagcgc cggatgtgtt ttcggtaagg
    11161 atcgtccatc gtgttacgag cgtcattata ccgcagccgc gccaccaccg gcgcgactct
    11221 gcgcgccttt ggcaaaaacc cttcgcgcag ttatagttcg ctgcgtgtac gccagccgcc
    11281 gccaacgccg acagcgacag cgcacgttgc tcgccgcggc gttcggtgtc ctactcgtag
    11341 caatcctggc gctcgccgcg tggagcctca ccggctcgcg ccccgagacc gcgtcggccg
    11401 aacccggcac ctacgcgcag gtctctacgc cccgtaccgg cgagaaggac gatctcacga
    11461 cgtggggcga cagtgccgat cccgtcaagg cgatctacct gaccgcctat aatgccggat
    11521 ccgcgaacct ggaggcgtac ctcaagctac tcgaccgcac ggagttgaac gccgtggtcg
    11581 tggacgtaaa ggatgtcacc ggcgaggtga tgtacccctc caaagtgccg ctggcgaatg
    11641 agatcggggc gacccggcag gtgctcgacc tgaagtcgct cacgaagcaa ctcaaggagc
    11701 gagacgtcta cgccattgcc cgcgtcgcgg tgttcgagga cgacatcctg ccacgccaga
    11761 ggccggacct ggccgtcacc gactcgtcta ccggagcgcc ctggctcaac gacgccggtc
    11821 agacgtgggc gaaccagtac aacaaggagg tctggaagta caacgtggcg gtagcgaaag
    11881 aagccgcgag ggccgggttc gatgagatcc agttcgacta catccgcttc ccttccgacg
    11941 gcccgatgga gaccatcgac tacggcaagg agacctaccc taaacaacag gaagctctcg
    12001 ccgccttcct gaagtacgcg gacagcgagc tgcacccgat gggcgtgcgg gtggcgtccg
    12061 acgtgttcgg gctcgccgcc acggaggacg gagccggggt cggccagtac atcgacacgc
    12121 ttgcgccgta cctggacgtg gtctgtccca tggcataccc ctcccactac ccgcccggca
    12181 gctacggcta cgacgacccg aactccgagc cgtacaaggt catcgagcat acgatgacgg
    12241 acttcgagaa gaagaccaaa gacgcgaacc cgggcatcga gatccgtccg tggctccagg
    12301 acttcaccct cggaactccc ccctacggac ccgaggaggt tcgcgcccag atggacgctg
    12361 tctacgactc cggcgcgact ggatggctcc tctggaacgc tgacaacgac tacaccgaga
    12421 tggcgctcaa ggcctccagc cgatagaggc agaacaaagg cccggccgag atggccgggc
    12481 cttcttggtg cggtgttctc gggtgcgtct aggccagtcc ctcggcctcg tactcgcgga
    12541 ggaagccctc gaagagcttg cggtggccct gctcgtcgcg caggatggcg atgaccatgt
    12601 cctgcgttac cgggtcgacc tcctccgtag cctctatcag gcgggtgtag tgctggatgg
    12661 cgccggtctc ggcctcgatg acgcccttga tcacgtggac cacgtctacc tggtcttccg
    12721 gcggctgcag gaagctctgc tccgccacga actccgccga gccggggacg acgccgtaca
    12781 actcctttat gcgcgccgcg aagtcctgcg cgtgctgcaa ctcctcctgg atatcctcct
    12841 ccagggactc cttgatctcc tgcgctcgca caccatcggg gttggtggaa ttcgtgacgt
    12901 agttgatgac cgtctcaagc tccatgaaat acgccttctt gagaagctcc acgatctcct
    12961 cgcgtttccc ggccatctct tccgagagta tcccctcgct accgcgattc gtctccacgt
    13021 ctttgacctc ctgtgtgatt acaagaatct tacggaattt actttaccct cgcgactcgc
    13081 ccataaacgt gaacttgcga acacccgaag ggtgccccgg caaacctcgg acaccacgtt
    13141 caaacccgca cggtcttcct cttgcggaag tctttcggcc ggtagcgctg cacgtactcc
    13201 cggtacttcc tgacctcgtc tttcgaccgc tccgaggacc accccaggta cctgacggcg
    13261 acttcggccg ctgcggagtc caggtcgagg gccacccgcg gccccattcc gaccatcgtc
    13321 cgccggagca ggacatccga gagcgtctcg gccatctccc gccggaaagc gtagagcacc
    13381 tccgccccga ttatcccggt ctcgaccgta gccgaagccg agagcggcat cttgagcgag
    13441 tggtcgtcgc gggcctcggc gatcacgtcc ggggcgcggg taccgtagag cttgagcagg
    13501 cgctcggcga gcatgtcggt gagcccgctc gtggccttga actcggcggc gaagttgtcg
    13561 aagtcctcgg ccgcgccgcc ggggagcggg accttgtctg tccggcacgg cggcgcgtgg
    13621 aggtggagct tgtcgtagac gacgtcaacg gtctgccggg cgaggttgcg gtacgtggtc
    13681 agcttgccgc cgacgatgga gatgaggccg tccgccttcg gtccagcccc ggagacggtg
    13741 agcctgcccc cggcggcgga ggcgcctgtg gcgtggtcgt agacgacatg gctgcgggtg
    13801 atggagctct ccgagccctc cggcttgaac ggcaggggcc ggactccaga gtaggtaaac
    13861 aggacgctct cgcgggtgag ggcggcctgc gggaccaccg cgttcgtctc gtctatgagg
    13921 tattggatct catcgtcgtc cgccgacacg tagtcgaggt cccccctgta gcggaagtcc
    13981 gtggtgccga tgagatagcg gccgttccac ggaacgatga agtacggccg tccgtccctg
    14041 cgcgcctcca cgtaaagggc gtctcgcggc gcgccaggga acgcatccac cacaatgtga
    14101 gagcccttcg tgcccccgat catccgcccc gagtcctcct ccgcgccgtt ctccggcgcc
    14161 tcggcgaacc ctgacgcccc cgatccgcgc agcacccgat ccacccacgg acccgcgacg
    14221 ttgacggtta ccggagcatg ggccgtgtgc gtgccgcctc cgagcctgtc ctcgaactcc
    14281 acccccgcca cgcgggagcc gccgtcctcg aagagcaggc gcttcacctc ggcataagtc
    14341 agtacggtcg cgccgtgctc gcgggcggag atggcgttct ccaccgcgac gcgctcggcg
    14401 tactctacct gcccgtcgta gtaggtggcg gcgccgcgca ggccgtccgg gttgattccg
    14461 gggtattgct ctagagcctc gtcgcgggag agcatcttgt ggttcgggac cgacttatcg
    14521 aatgagagag cgtcgtagcc catcatgccg agccggatga gaccgggtcc gcgtttggac
    14581 cgctcgtaga tcgggaccac gaacttgagc ggcttgacga ggtgcggcgc gatgtgcaag
    14641 agcttctcgc gctcggcgag cgattcgcgt accagatgta cctcgtagtg ctccaggtag
    14701 cgcaggccgc cgtgtatgag gcgggtggcc cactgggtag tgccgctggc gatgtcgccc
    14761 ttgtcgagca gcaggacttt cagcccgcgc atggcggcgt cgcgcgcgat ccccgccccg
    14821 ttgataccgg ctccgaccac gatcaggtcg aacggatagt cccgtatgtc ctccggtacc
    14881 tgacggtcca tgcgctctcc tccttgtcaa tcgtgcagct tggactccgt gcgagcctcc
    14941 atcgcagcct ccacgcactc cccggcatcc gccccggtcg aggccatcac ccccgccgcg
    15001 aacgctccct ccacgaacgg cgcgtccacc agccgcacct tctctctcgt ctccgaggat
    15061 agcatctcga gcaccgtctc ggccgagagc accgcgctcc cgatgtccat gaagacaagg
    15121 acttcttcgg ccgtcagtcc ctctatagcg gcctgcacgc cctccgggtc ggtccccagt
    15181 ccgccgtcag agtcgccccc gacagcgacg acctccacgt ccccggccat gtgcccgatc
    15241 agctcggccg tgccgtcggc gatccggggg ctgtgcgaga cgagcacgag tccgaccatt
    15301 actcgctctc cagagcgcgg gccgccgcgt ccagcaggaa ctgcgtggag gtcgcgccgg
    15361 ggtcccggtg ccccgccgcc cgagcgccca ggtagctcgc ccgaccccgc ttcgccgtca
    15421 tcggcaccgt ggactccgcc ccctcctcgg ccgcgtccgc cgcagcccgg aagacctccg
    15481 ccgcgctccc gccgctggct cccttcgcag cctcgaccgc cggtgtcaga gcatccacga
    15541 tggtcttgtc tccaacctcg gccttgccgc gctgctttat cccgccaagc gccgcctcca
    15601 gcgccgccgc agtgtcctcc ccggagacct cttccttatc cccgagcgcc gtcgaagccc
    15661 gcaggaacgc ggtgccgtac agaggccccg ccgcgccgcc gaccttgctg atgagcgcca
    15721 tcgagacggc cttcaggaca tcggccgggg agtttagttc ggctccgtcg aggcgttcca
    15781 gggcggcctg gaagccgcgg tgcatgttcg taccgtggtc tccatcccca atctccgagt
    15841 cgagcttcgt taaatagcgg cgatgttcct ccatcgcggc ggccatctcc cggatcacgt
    15901 tcagcgttgc ttccgtcgct cccaaatcta ctcctccgat tccattagct tctcgcgcac
    15961 ctcgtgtttt aacgtcttgc cgagcgcgtt tcgcggcaac tcgtccacga acgcgacctc
    16021 gcggggcttc ttgtagcccg caagctcctc gcggcagaac tccgtcagct tctcggcggt
    16081 cagcgagggg tcgtcgcgca cgacggcggc gaccacccgc tccccgtact ccgggtccgg
    16141 caagccgacc acggccgcct cgacgacgcc ggggaacctc tccaggacct cctccacctc
    16201 gcgcggatac acattgtatc cgccggagat gatcaactcc ttcgcccgcc cgctgatggt
    16261 gtagtacccg tcctcgctca cggagccgag gtcgcccgtg cggaaccaac catcctcgta
    16321 gaagctctcc tccgtggcgt ccggacggtt caggtagccc gcgaagacgt gcggccctcg
    16381 aacctcgatc tcaccgtcct cacccggcgg tagtagtttt cgagtctcca ggtccacgac
    16441 ccgcgcctcc tgtccgggga acgggcctcc aaccgttccg ggccgccgct cgccgtcgaa
    16501 cgggtttgtg aggttcatga tcgtctcggt catcccgtac cgctccagta tgcgctcccc
    16561 gaacagccgc tcgaactcct cgaacgcctg cgggctcaac ggcgcgctcc ccgacacgta
    16621 cagccgcagc ggagccgggc gctcgtcccg cttctccgcc tcccggagca ggcgcgtgta
    16681 catcgtcggc accccgaaga acatcgtgac ccatccctcc agcagcgctt cgtagacctt
    16741 cgcggcgtcg aagcggcggt gcagctcggc gctcgccccc atgtagaacg tgccgtgagc
    16801 gccgaccatc aagccgtggg tgtggaagag cgggagcgcc agcagcaggc gatcctcctc
    16861 cgtccagcgc cacgcctcgc agacggcggc ggcgttggcg gcgaggttcc ggtgcaggag
    16921 catcgcgccc ttgctgcggc ccgtggtccc ggaggtgtag gcgacgacgg caagatcttc
    16981 accgtccggc atcccgaact tccgctcctc cccggagccc aggaagtcct ctaactcctc
    17041 cccgacctcg acaacggtct ccagatgcgc gaggtcctta cggacccgtt ccagttcaac
    17101 acgccgctcc gcgtccgtga ggcagaggcg ggcgccggag tcctcgaaga tgtggcgcag
    17161 ctccgtccgc cggtactggg tgttgaaggg cacgaccacg ccgccggaga gatgcgtccc
    17221 gagataggct gcgaggagat ccggacagtt ctccagaaag agcgcgaccc ggtcgccggg
    17281 tcccaggccg cgtccacgga gcgcggaggc gaaggcttgc gcgcgctgac gcagacttcc
    17341 gtaggtccac tcctcgccct cgaagcgcag gcagagtctc tccggcgcgc gctcggcgtg
    17401 acggataaag ctctccacga tggatgccgg tgaaccgccg ttcacctaga gccctcctcc
    17461 gacggagcgg gcgcgttcga ggagaccttc gacgaaggcg tcgtcccacc agccgaggaa
    17521 gaagagcagg tccgcgacgg tgaaccggtt tcgcatcagg tggcagttcc gcagcgccca
    17581 gaaggcggtc tccgcgggga ccggcgggtc caggtctctg aagcgcgcgg gagccccgga
    17641 ctcttgcagg gccttaccga gccactcagg agagaagacc atctcccgca gctcgttccg
    17701 gagccgggac cagttcgcga agaactcttc gaagcggggg cgcgcagtcc gccagcgctc
    17761 cagcttcagg gcgtaatcgt tccagcactc ttccgctatc gctcctgtcg ggtccaggtc
    17821 cgagaaggcg tcgcgcacca tcggctccac ggcttcgggg tcggggaagc aggcgtctat
    17881 gtcgatctcc gcggggtcca gccggaagaa gagttcttcc caggcggcgg cgacggggac
    17941 cgtggcgatg ccgacctggg cgccgtggaa gttgaacggt aggccgcgtt tgccggcgtt
    18001 catgtcgagc aggtggctga ccaggtgctc ggtgccggag aggggcgcgg tcttgccgat
    18061 cacacccgcc gccatcccgc cgagggccag gcagcgggcg agcttctcca gcgcctctgg
    18121 ctcgtgacgc cgcacggccg cggcgccgtc gagcacctcg cgggcctgat cgaagaccat
    18181 gttcagcggc gccgggttgt aggagtcgtc catgccaacg agcgcggcca ggtaccagtc
    18241 cgccggggag gtccaggtcg ccagcaggtc gccgaacccg gccaggctca tgacacgcgg
    18301 cgctccggca aggatgggaa ggtctatgag gagggcgtcc ggccacttgg aggtcccggt
    18361 ccgcttaact ccgctcttga gcagcacgga catgttgatg gcgaaggcgt tgaccgaagc
    18421 ggcggtctgc actaccacga gcggtatgtc tcccttcctg gccgtggcct ccttgcagag
    18481 gtcggtgatg gtgccggagc cgaccgccac cacgcagtcc gccccctcta cggcggcctc
    18541 gacctccgcc agcgcctcct cgtcggcgtg cagttcgtgc tcgtcgtcgc cgatgaccac
    18601 gcgtcgcacc tcaaagatct cgcccagcgt ccgctcggcc caatccttca ggtcctcccc
    18661 gctgcggagt atgggcgtcg agtcgcacac gagcacgacg ttcctgccgc gggcgacctc
    18721 ggagactacc tcgctcaaga gttcgatggc ttcgggtcct atctcgatcc ggcgcatctc
    18781 gatagggttg agtgcgcctt ccgggtccgc ggccgccagc gcctcgcgca tggagtcgag
    18841 gtccgtgggg tcggctaggg gtagcctgtc ggcggcgttc atatgagtag cttccttcca
    18901 tccggggtcc tggcgggtct cattccgccg actcggagac cgacgttggc tggccgtatc
    18961 cctggatctt ggcggccacc tcggagatct gctccgggtc caggaggacg ggttccccgg
    19021 cgaggcgcgt ccggtagtag atctcggcca tctcttcgag gaccacggtg cgggcgaacg
    19081 ccttttcggc gttctctccg acggcgatcg tgccgtggtt cgccagcagg catgcgttgt
    19141 gggactcacc cagggcgctc gcggcgttcc cggccagttg ctcggtgccg tagagcacgt
    19201 atggcgccag cgggatgcgg ccgtccgggc cgagggtggt tagcatgtag tggaccggtg
    19261 gtatctccca accgaggcac gccagggtgg tggcgaagcg ggagtgggtg t
//
LOCUS       DASSMB010000272         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_609029, whole genome shotgun sequence.
ACCESSION   DASSMB010000272 DASSMB010000000
VERSION     DASSMB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609029"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1718)
                     /locus_tag="VFJ72_15515"
     CDS             complement(<1..1718)
                     /locus_tag="VFJ72_15515"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7480912.1"
                     /translation="MEAATTSAEIGSRTGARLIAEFLARRGVRTIYGLCGGHVQPIWD
                     EAARAGIRIVDVRHECAAVYMAHAQADLTGSLGVAMVTAGPGLTNAITGIANASVSRS
                     PVLVISGRPPRPQAGMGGLQDIPQGELIKPICRRVEAVYESHHILPRLEAAVRAAEGV
                     GGPAGPAYIDFPTDLLRADIPDADVDDLFFEPRPRDEPGPSEDAVTRAASVIREGKRL
                     LVISGKGARAGAKELSDFLDASGALYLDTAESRGVIPLGHPASVPASRAKAMAEADVI
                     VTVARKLDFQLAYGSRAVFPEDAKFVRLGASGDELFDNRRGDAEVLGDVAASLRALIE
                     AGAVPERPDTEWVESLKSYDREKSEKFLARMGKQPPGSDGYMHPSSLLAAVNEFIDED
                     TIVVADGGDILSFARGGLQAETYLDPGPLGCLGVGVPFANSAALNFPERRVIAVIGDG
                     SFGLTAMEIDTAVRSGARAVFVVANNESWAIERNDQLVGYGGNLVGVDLPGCRYDRLA
                     EGLGAYAERVEKAEDLPGALERAFENAPALLDVAVTKEAQSPDFKNGLAWVPDRQALE
                     VWDQAE"
     gene            complement(1830..2249)
                     /locus_tag="VFJ72_15520"
     CDS             complement(1830..2249)
                     /locus_tag="VFJ72_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012117385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HET7480913.1"
                     /translation="MQKLYGASVTAEGDRKGRVVSSDGVLDLDLSMPEGLGGPGGDGT
                     NPEQLFAAGYAACFESAIRLVARQEKVDVTGASVTANIDIGHPPEGGFALEAELVGRM
                     PNLSRAEAEGMMQKAHQICPYSKATRGNMEVKLSVAE"
BASE COUNT          336 a          870 c          721 g          380 t
ORIGIN      
        1 ttctcggcct ggtcccacac ctccagggcc tgtcggtcgg gcacccaggc cagcccgttc
       61 ttgaagtcgg gcgactgagc ctccttcgtg acggccacgt ccagcagggc cggggcgttc
      121 tcgaaggcgc gttccagagc gccgggcagg tcttcggctt tctcgacccg ttccgcgtac
      181 gcgcccagcc cctcggccaa cctgtcgtag cggcagccgg ggaggtcaac cccgaccagg
      241 ttgccgccgt atccgacgag ctggtcgttg cgctcgatgg cccacgactc gttgttagcc
      301 accacgaaaa ccgcccgggc gccgctacgg accgccgtgt cgatctccat ggcggtcagg
      361 ccgaacgagc cgtccccgat caccgcgatc acccgccgct ccgggaagtt cagggccgcc
      421 gagttggcga acgggacgcc gacgccgagg catcctaaag gaccgggatc caggtaagtc
      481 tccgcctgca aaccgcccct ggcgaacgag aggatgtccc cgccgtccgc caccactatg
      541 gtgtcctcgt cgataaactc gttgaccgcc gccaggaggg acgagggatg catgtacccg
      601 tccgaacccg gcggctgttt ccccatccgc gccaaaaact tctcgctctt ctcccggtcg
      661 taggacttca gggattcgac ccactccgtg tccggccgtt cgggaacggc gcccgcctcg
      721 atcaatgctc gcaaggacgc cgcgacgtcg cccaggacct ccgcgtcgcc gcggcggttg
      781 tcgaagagct cgtcgccgct cgcccccagc cgcacgaact tcgcgtcctc cgggaacacg
      841 gcccgcgaac cgtaggcgag ctggaagtcc agcttgcgcg cgaccgtgac gatgacgtcc
      901 gcctccgcca tcgctttcgc acgggaagcc ggtacggagg cgggatggcc cagcgggatc
      961 accccacggc tctcggccgt gtcgaggtaa agggcgccgc tggcatccag gaagtccgat
     1021 agctccttgg cgcccgccct cgcgcctttg cccgagatca cgagcagccg cttgccctcc
     1081 cgtatgacgg acgccgccct agtgaccgcg tcctccgagg gaccgggctc gtctctgggg
     1141 cgcggctcga agaacaggtc gtccacgtcc gcgtcgggga tgtccgcccg gagtagatcc
     1201 gtggggaagt ctatgtacgc cgggccggcc gggccgccca cgccctcggc cgcccggacc
     1261 gccgcctcca gccgggggag gatgtggtgg ctctcgtaga ccgcctcgac cctccggcag
     1321 atcggcttga tcaactctcc ctggggtatg tcctggagcc cgcccatccc ggcctgcggc
     1381 cgcggcggcc tccccgagat gaccagaacg ggggaacgcg agacgctcgc gttggcgatg
     1441 ccggtgatgg cgtttgtgag tccggggccg gcggtcacca tcgccacgcc cagcgacccc
     1501 gtcaggtccg cctgggcgtg ggccatgtac actgccgcgc attcgtggcg cacgtccacg
     1561 atccggatgc cggcccgcgc ggcctcgtcc cagatcggct gtacgtgccc tccgcacagc
     1621 ccgtatatgg tgcgcacgcc ccggcgcgcc aggaactccg cgatcaggcg ggctcccgtg
     1681 cgagagccga tctcggcaga agttgtcgcc gcttccatca ctgcctcctg ttttcagatg
     1741 ttggcgatac tcaaactggc cctatccctc ccccaagaga cccgcatccc cggcggcctt
     1801 gagcgccccc gccgtggacc gaccccggct cactccgcca cggagagctt gacctccata
     1861 ttgccccgcg tggccttgga ataggggcag atctggtgcg ctttctgcat cattccctcg
     1921 gcctccgccc tcgacaggtt tggcatacgc ccgaccagct cggcttccaa cgcgaaaccg
     1981 ccctcgggag ggtggccgat gtctatgttc gccgtcacgg aggccccggt cacgtcgacc
     2041 ttctcctgac gggcgacgag tcgtatggcg ctctcgaagc acgcagcata cccggcggcg
     2101 aagagctgct cggggttcgt gccgtccccg ccgggcccgc caagcccctc cggcatcgag
     2161 aggtcaagat ccagcacgcc gtccgaggac accacgcggc ccttgcggtc cccctccgcg
     2221 gtcacgctcg cgccgtacag tttctgcatt gttcctcctg tctttcgtgt accgctatct
     2281 cgccaccccg cgcccggcgc ggctcgg
//
LOCUS       DASSMB010000273         7117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_719771, whole genome shotgun sequence.
ACCESSION   DASSMB010000273 DASSMB010000000
VERSION     DASSMB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7117
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719771"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFJ72_15525"
     CDS             <1..333
                     /locus_tag="VFJ72_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480914.1"
                     /translation="MSGVTTGDAGLASGLINTTAQVGGALGLAVLATLSASRSSELIR
                     DGQPTAAALTSGYHLAFGIGAALVAVAIAVAVTVLKDERRAEQGAGLDPDPWELEDPD
                     EQEDENAA"
     gene            323..1099
                     /locus_tag="VFJ72_15530"
     CDS             323..1099
                     /locus_tag="VFJ72_15530"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7480915.1"
                     /translation="MLLEDRNAVIYGAGGAIGGAVARSFAREGARVFLAGRTLARLDA
                     VAGEIRSAGGVAETAQVDALDERAVEKHVGEVVEQAGSIDVSFNAISIRDVQLIPLVE
                     MSREDFVSPILTGATTHLLTARAAARRMTERGSGVILTLSASAVRAYVPGVYVGGFGV
                     ACAAIEALTKQLAAELGPHGIRVNCLRSEGIPESWKGASTEGWSAPTEEIEAQMKARS
                     LLGRVTTLADVGNAAAFLASDLAGATTGTVFNLTSGTVVD"
     gene            1190..1600
                     /locus_tag="VFJ72_15535"
     CDS             1190..1600
                     /locus_tag="VFJ72_15535"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7480916.1"
                     /translation="MSMDLRLEVVPIPVSDVDRAKRFYGEQVGFIVDLDTLIGDEMRL
                     VQLTPPGSGCSIHLSAGILDMPPGVLEGLQLVVADIEAARTELVERGVEAGPVQHMDG
                     GLWVDGRGGDWNSFVFFSDPDGNGWVLQERPARA"
     gene            1622..2242
                     /locus_tag="VFJ72_15540"
     CDS             1622..2242
                     /locus_tag="VFJ72_15540"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7480917.1"
                     /translation="MGKVSIGLSMSLDGFIAGPNDGPGSPLGDGGERLFAWYSGGGTE
                     YRLPGTEMVFEVSPQSAELLREAHGKMGAFVTGRRTFDITTGWGGRPPLGVPTFVVTH
                     AIPQEWVHEGSPFTFVTDGVESAVEQAREAAGDKDVAVGAASIAQQCIRAGLLDEIHV
                     DLVPVLLGGGVRLFDRLGAEPIELERTRVVEAPGVTHLTFRVVRED"
     gene            2244..2804
                     /locus_tag="VFJ72_15545"
     CDS             2244..2804
                     /locus_tag="VFJ72_15545"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7480918.1"
                     /translation="MRKVIVSEFVSLDGVMEDPSWTFQFTSEEQPKFKLDELAAADAL
                     LLGRVTYEGFAAAWPSMTDEEGYADMMNSYPKYVVSETLEEPLEWNNSTVIKGNVAEE
                     IRKLKQQDGKDILVFGSAALVNTLMERDLIDEYRIMIFPVAVGSGKRLFTEGSDTKIM
                     RLVEMRTFGSGVVVLSYRPAGKEAEG"
     gene            2837..3232
                     /locus_tag="VFJ72_15550"
     CDS             2837..3232
                     /locus_tag="VFJ72_15550"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7480919.1"
                     /translation="MSLSDYRVAAALAVSDMDRAREFYEGKLGLSVGMDSGDNLQYWC
                     AEGTMIHVYLSPEHAGKSTATLAGWGVDDVERVVDELASKGVIFERYDKGPIITDDKG
                     IATFDRGARVAYFRDPDGNTLSLAQNPRP"
     gene            3462..3845
                     /locus_tag="VFJ72_15555"
     CDS             3462..3845
                     /locus_tag="VFJ72_15555"
                     /inference="COORDINATES: protein motif:HMM:NF015298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HET7480920.1"
                     /translation="MHKKLLLSALVAAILAFYASPAMAYPQEVSWYGYSPVTGASDGS
                     DGSVTASGEMFDSQEYTCAAGYDYPFGTLLKITYEGRSVICRVNDTGDYQGRWDLSYA
                     AAKEIGLADAADVAVVDVEVASQPE"
     gene            complement(3870..4793)
                     /locus_tag="VFJ72_15560"
     CDS             complement(3870..4793)
                     /locus_tag="VFJ72_15560"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7480921.1"
                     /translation="MGVVRRPVDERGLVGALFHPPSSAAHPAVVALGGAGGGLSEGGA
                     RTLASEGFAALALAYFGTGSLPEDLVEIPLEYFERAIAWLKAQPAADADRVAVVGNSK
                     GGELALLLGATYPEEIRAVVGYAPSGVVWQGNSFDREVYHWGPRSPWSLRGDPVPFAG
                     LPGPRVSEMVRLAESYFDGQLISLRPFYERALEDEASVAAASIAVEKIEAPVLLVSGA
                     DDRLWPSTRLSEMVMARLDAHGHPFSREHLRYEGAGHLISLPGYVPARSWANRFELGG
                     SREANEFANADSWTKVLGFLKEHLGDEGPPA"
     gene            complement(4919..5212)
                     /locus_tag="VFJ72_15565"
     CDS             complement(4919..5212)
                     /locus_tag="VFJ72_15565"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HET7480922.1"
                     /translation="MNFVADEGVDRQIMDRLRNEGHAVIYVAEMEPGITDDSVLNAAN
                     KESAVLVAADKDFGEIVFRQGRSSSGVVLIRLAGISPDARARRTRITTTDTRG"
     gene            complement(5209..5445)
                     /locus_tag="VFJ72_15570"
     CDS             complement(5209..5445)
                     /locus_tag="VFJ72_15570"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET7480923.1"
                     /translation="MATNDLIQSDPEVLMGKSVVSGTRITVELILEKLAAGESIEQIL
                     DEHPRLSRGSVLAAISFASEALRADVVYPTRDTA"
     gene            5873..6325
                     /locus_tag="VFJ72_15575"
     CDS             5873..6325
                     /locus_tag="VFJ72_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7480924.1"
                     /translation="MSNDFILLADDNTRSADLFVKVLRMRDIGTGVVLARDGAETLDY
                     LFGEGEHAGRDTSVMPRLVILDLNMPGMDGLEVLTRLRADERTALLPIIIFSASGTTR
                     DMAQAYRMGANAYVDKVSSTTFPEAVWHMVYFWLNVNQPPPVLEHLPS"
     gene            complement(6695..6907)
                     /locus_tag="VFJ72_15580"
     CDS             complement(6695..6907)
                     /locus_tag="VFJ72_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480925.1"
                     /translation="MARGEIIFAVEESPEGGLEARALGHSIYSEADTMEELKEAVRDA
                     VRCHFDQEDLPSVIRLHIVRDELLSV"
BASE COUNT         1296 a         2246 c         2300 g         1275 t
ORIGIN      
        1 atgtcgggcg ttacgacggg cgacgcgggt cttgcctccg gcctgatcaa cacgacggcg
       61 caggtcggtg gcgccctggg attggccgtg ctcgcgaccc tgtcggcgtc gcggagtagc
      121 gagttgatcc gggacggcca gcccaccgcg gcggcgttga cgagcgggta ccacctcgcg
      181 ttcgggatcg gggccgccct tgtcgccgtg gctatcgccg tcgccgtaac cgtgctaaag
      241 gacgagcgcc gggcagagca gggagctggg ttggatcccg acccctggga gcttgaagat
      301 ccagacgagc aggaggatga aaatgctgct tgaggacagg aacgccgtga tctacggagc
      361 gggaggggcc atcggcggcg cggttgcccg ctccttcgcc cgcgaggggg ccagagtatt
      421 cctcgccggt cgtacccttg caaggctcga tgcggtggcc ggggagatcc gctccgccgg
      481 aggagtggcc gagacggcgc aagtcgacgc cctcgacgag cgggccgtcg agaagcacgt
      541 cggcgaggta gtggagcagg cggggagcat agatgtctcc ttcaatgcca tctcgatccg
      601 ggacgttcag ttgatcccgc tcgtcgagat gtcacgcgag gacttcgtga gcccgatctt
      661 gaccggcgcg acgacccacc tcttgacggc cagggcggct gcgcggcgca tgaccgagag
      721 gggctccggc gtgatcctga cgctctccgc atcggcggtc cgcgcgtacg tcccgggcgt
      781 ctacgtgggg ggcttcgggg tcgcgtgcgc ggcgatagag gccctcacga aacagctcgc
      841 ggcggagctc gggccacacg gcatccgcgt gaactgcctg cggtcggagg ggataccgga
      901 gagctggaag ggcgcctcga ccgagggctg gtcggctcct accgaggaga tcgaggcgca
      961 gatgaaggcc cgttccttgc tggggcgggt gacgacgctg gccgacgtgg gtaacgcggc
     1021 ggcgtttctg gcctcggatc tggccggcgc gacgacaggg accgtcttta acctgaccag
     1081 cggcacggtc gtcgattagg agcacctcca tgaccggcac gttcgtcaat gtcaccggcg
     1141 gagtgttccc caactatatg atcagtgaaa tcaatccgaa aggaagcgaa tgagtatgga
     1201 cctccggctc gaagtggttc cgattcccgt ctccgacgtg gaccgcgcga agcgtttcta
     1261 tggcgagcag gtcggtttca tcgttgacct cgacaccctg atcggcgacg agatgcgact
     1321 cgtccagtta accccgcccg gatctggctg ctcgatccac ctgagcgccg ggattctcga
     1381 catgccgcca ggggtgctgg agggcttgca actcgtcgtc gccgacatcg aggctgcccg
     1441 gaccgagctg gtcgagcggg gcgtggaggc cggcccggtc cagcacatgg acggcggact
     1501 ctgggttgac gggcggggcg gagactggaa ctcgttcgtc ttcttcagcg atccggacgg
     1561 caacggatgg gtcctgcagg agcgccctgc ccgcgcttag gccatcgaaa ggagaacgaa
     1621 catggggaaa gtctctattg ggttgtcgat gtcgctggat ggcttcatcg ccgggccgaa
     1681 cgatggcccc gggtcaccgc tgggtgacgg aggtgagcgg ctcttcgcgt ggtactcggg
     1741 cggcggcacc gaatacaggt tgccgggtac cgagatggtg ttcgaggtct cgccgcagag
     1801 cgccgagctc ctccgggagg cgcacggcaa gatgggggcg ttcgtgacgg gacggaggac
     1861 gttcgacatc accaccgggt ggggtggcag acctcccctg ggcgtaccga ccttcgtcgt
     1921 tacccatgcg atcccccaag aatgggtcca cgaaggatcg cctttcacct tcgtcacgga
     1981 cggtgtcgag agcgccgtcg agcaggcgcg ggaagccgcg ggggacaagg acgttgctgt
     2041 aggcgccgcg agcatcgcgc agcagtgcat aagggctggt ctgctcgatg agatacacgt
     2101 cgaccttgtg cccgtcctgc ttggaggggg cgtccgcttg ttcgaccgtc tgggcgccga
     2161 gccaatcgaa ctggaacgca cgagggtggt agaagcccct ggtgtaaccc acctcacgtt
     2221 ccgcgttgta agagaggatt agcgtgagaa aagtaatcgt atcggagttc gtgtcgctgg
     2281 acggcgtgat ggaggatcca agctggacct tccagttcac gagcgaagaa cagccaaagt
     2341 tcaagctcga cgaactggct gcggccgacg ccctcctgct ggggcgggtg acctacgaag
     2401 gtttcgcggc cgcctggcct tccatgaccg acgaagaggg ctacgccgac atgatgaaca
     2461 gctaccccaa gtacgtcgtt tcggagaccc tggaagagcc gctagagtgg aacaactcga
     2521 cggtcatcaa ggggaacgtc gcggaggaaa tccgtaagct gaagcagcaa gacggcaagg
     2581 acatcctggt cttcggcagc gccgccctcg tgaatacgct gatggagcgc gatctcatcg
     2641 acgagtacag gattatgatc ttccccgtag ccgtgggtag cggaaagcgc ctcttcacag
     2701 aaggtagcga tacaaagatc atgaggcttg tagagatgag gacgttcggt tcgggcgtcg
     2761 tcgttctctc ctaccggccg gcaggaaagg aggcagaggg atagggaccc gaattacggg
     2821 atcggaggag gctcaaatga gcttgagcga ctacagggtg gccgcggcgc tcgcggtctc
     2881 cgacatggac cgcgccaggg agttctacga agggaagctc ggactttcgg tgggcatgga
     2941 ctcaggcgac aacctgcaat actggtgcgc ggaagggacc atgatccacg tctacctctc
     3001 accggagcac gccggcaagt ctacggcgac cctggccgga tggggcgttg acgacgtcga
     3061 gagagtcgtc gacgaacttg cctcgaaggg cgttatcttc gagcgttacg acaaagggcc
     3121 gatcataacg gacgacaagg gcatcgccac gttcgaccgc ggcgccaggg tcgcgtactt
     3181 cagggacccg gacggcaaca ccctctcgct cgcccagaac ccccgtccgt gaacgccatc
     3241 ggcgagttcc tctcgcggcg ccggcgggat ctggccgggc tggccccaac cgtaccggac
     3301 ccggcgtgaa gcgccacccg tagtacgcct cgcggagtct ccgcaaccgg ggtcgcctct
     3361 ttgtgagctt gcgatccctt tcacatctac ttctggtcac atccttgctc cgtggtaggg
     3421 tctccgcccg ctatgcaagt tagtgagggg tatgtttctc tatgcataag aagctgctgt
     3481 tgtccgcgct cgtcgccgcg atcctggcgt tttatgcgtc gcccgcgatg gcgtatccgc
     3541 aggaggtctc ctggtacggt tactctcccg ttaccggcgc gtcggacggc tccgacggga
     3601 gcgtcaccgc ctctggagag atgttcgact cccaggaata cacgtgcgcg gcggggtacg
     3661 actacccgtt cggcacgctg ctcaagatca cctacgaggg ccgctccgtt atctgccggg
     3721 tcaacgatac cggcgactac cagggacggt gggacctctc ctacgccgcc gcgaaggaga
     3781 taggtctcgc ggacgcggcg gatgtcgccg tcgtggacgt ggaggtcgct tcccagcctg
     3841 agtgatcccg gcccgggaga ggaccgccgc taggccggcg gtccttcgtc cccgaggtgc
     3901 tccttcaaga agccgagtac cttcgtccac gagtcggcgt tggcgaactc gttcgcttcc
     3961 cggctcccgc ccagctcgaa gcggttcgcc caggacctcg ccggcacata gcccggcagc
     4021 gagatcaggt gtcccgctcc ctcgtagcgc aggtgttcgc gggagaaggg atggccgtgg
     4081 gcatccaggc gagccatcac catctccgag agtcgcgtcg agggccacag ccggtcgtcg
     4141 gcgccggaga ccagcagcac gggagcctcg atcttctcca ccgcgatgct cgccgcggcc
     4201 acggacgcct cgtcctcgag tgcccgctcg tagaaaggac gcaggctgat cagttgaccg
     4261 tcgaagtacg actccgcgag ccggaccatc tcggagaccc ggggacccgg cagcccggcg
     4321 aacggcacgg gatctccccg cagagaccag ggagacctgg gaccccagtg atagacctcg
     4381 cggtcgaaag agtttccctg ccagacgacc ccgctcggcg cgtacccgac gaccgcccgg
     4441 atctcctccg gatatgttgc cccgagcagg agcgccagct cgccgccctt cgagtttccc
     4501 acgacggcca cgcggtccgc gtcggccgcg ggttgggcct tcaaccacgc gatggcccgc
     4561 tcgaaatact ccagggggat ctccaccagg tcctccggca gcgagccggt cccgaagtag
     4621 gcgagcgcca gagcggcgaa cccctccgag gcgagcgtcc tggctccgcc ctcgctgagg
     4681 ccgcctcccg ccccgccgag ggccaccacc gccgggtgcg cggcggacga gggcggatgg
     4741 aacagcgcgc cgaccagccc cctctcatcc accggccttc gaaccacccc catctctcgc
     4801 acgaatacat gctaccggac tgcgcaaccc cgcgtaagat ggccgctccc gtcaggcccg
     4861 ccgcgtaaac accacgccgc aagaggctct ccatcatggt cttctcgaac cttccgggct
     4921 atcctcgtgt atcggtggta gtaatgcggg tcctccgagc cctggcgtcg ggagatattc
     4981 ccgcgagccg gatcaggacg accccgctgc tcgagcgtcc ctgacggaac acgatctccc
     5041 caaagtcctt gtcagccgcg accaggaccg cggactcctt gttagcggca ttcagcacgg
     5101 agtcgtccgt gatgccaggt tccatttcgg caacgtagat caccgcgtgt ccttcgttcc
     5161 tgagccgatc catgatctga cggtccacgc cttcatcggc gacgaagttc aagccgtgtc
     5221 gcgcgtagga taaactacgt cggcccgcag agcctcggac gcgaaagaga tcgcggccag
     5281 cacagaaccc cggcttaacc ggggatgctc atccaggatc tgctcgatgc tctcacccgc
     5341 ggcgagcttc tctagtatca actccaccgt gatccgcgtg cccgagacca cggacttgcc
     5401 catcaaaacc tccggatcag actgaataag atcgtttgta gccactgcgc atcctcgcct
     5461 cggactccgc caacaactct attgtaccgg gacagagact ggcgccgtcg ggcgggacgc
     5521 tagcgtacat cctccagcta cagatggact taccgccatc gccaccggcg taccttccgg
     5581 gccatccccg gcgtctccgt ggcgatcatg cgtctgcaaa ggacccgata tttagaagct
     5641 ttggaagcga aggagtgtca tgatagagtc cgcgtagggc cgcgtggagc gaaattcttc
     5701 gattccagat cacccgccgt atctacgggg ctccatcctc cggggccgcc tcgactgtct
     5761 ccgggctgac ttagatcccg ggcgccgggg ccggatctcc gccgtcgcta aagttccgcg
     5821 gcgaacgtgc cgataactct tttaaggata tggatagctg gggagaggcg cgatgagcaa
     5881 cgactttatc ttactggccg atgacaacac caggagcgcg gacctgttcg tgaaggtgct
     5941 gaggatgaga gatatcggca ccggggtggt cctggcccgc gacggggccg agacgctgga
     6001 ctacctgttc ggggagggag agcacgccgg gcgggacacg agcgtcatgc cccgtctggt
     6061 gatactggac ctgaacatgc cgggcatgga cggtctggag gtgctgacgc gcctgcgcgc
     6121 cgacgagcgg accgcgctac tgccgatcat catcttctcc gcttcgggta cgacgcgaga
     6181 catggcccag gcctaccgga tgggagccaa cgcctacgtg gacaaggtgt cgtccacgac
     6241 gttcccggag gcggtgtggc acatggtcta cttctggctg aacgtaaacc agccaccgcc
     6301 ggtcctcgaa catctcccgt cctaaggacc tgcacgcccg ctgccgggag gaggagctag
     6361 ccggcgtctc ttttcgtcag ccctacccgg ccgggatcga tcaggagcag cggagtcggt
     6421 tcagtcggga atgccgatta ttatcgaact gccatttatc cgaatagctc tctgaccaaa
     6481 gtatcacggt ctatctccag gtgatccgcg acatccctga gtatgctgct tagcgtgcct
     6541 gctttcagag gcttgtgatc gggaatggtg acgtgatgtt tgttgccttc tcttttcgag
     6601 agtcgcatgt ggcggcccgt ctgccgggta acttcgtacc cgtactttcc aagtagtttc
     6661 gccagatctc taccgccgac atctctcgga agcctcatac ggacagaagc tcatccctta
     6721 caatatgcaa ccggatcacg ctcggcaggt cttcctggtc gaagtggcac ctgaccgcgt
     6781 cgcgcacggc ctcctttagc tcttccatcg tatcggcctc cgagtagatg gagtggccca
     6841 gggctctggc ctccaggccg ccttccggtg actcctcgac cgcgaaaatg atctcgcccc
     6901 gcgccatgca agctccttct ctccgacatg ttgtacccat atttaataac actttcccgc
     6961 ttcgtatatc tttcgtagac gctgttcctc gcctttgagc acagttggat cgttcagagg
     7021 aatacgcgta ctaggaaaac gtgaggatac atctcacatg gctgcggcga aggtcgatca
     7081 ggagcagcgg acccggaccg gaacagggcc agcaccg
//
LOCUS       DASSMB010000274         9509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1101607, whole genome shotgun sequence.
ACCESSION   DASSMB010000274 DASSMB010000000
VERSION     DASSMB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101607"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..1594)
                     /locus_tag="VFJ72_15585"
     CDS             complement(359..1594)
                     /locus_tag="VFJ72_15585"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7480926.1"
                     /translation="MLRLGLGPEYRKLWTASTISNLGDGITLVAGPLLAASLTRDPVL
                     VAGLTFAQQLPWLLFSLLSGALVDRLDRRLLMASVDAFRTGVIGILGLAVLTGWASLP
                     LMYGVFFLLGTAETLFANASLSIMPSMVQKEQLEKANGQMFAAELVTNQFAGGPVGGA
                     LFAVAAAFPFLIDAGTFAASSALILALRGRFRVVRPDGVQKAGILVEIAEGIGWLARH
                     RLLLALAVMLGAGNLTFSATFSVMVLFAQDTLGLGSFGYGVLMTAGGVGGFFGSLLAG
                     RVGGWIGTGRALFLVMLIQGPMFAGIALSGNPVVVALIWAVGGFAAFAWNVLTFALRQ
                     TLIPDRLLGRVTSVYRLAGVGSGAFGALIGGLLAKHFGLSAPFWFSAAVMILAALAFL
                     PFVNNRTVSEARRNANSRA"
     gene            complement(1598..2176)
                     /locus_tag="VFJ72_15590"
     CDS             complement(1598..2176)
                     /locus_tag="VFJ72_15590"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7480927.1"
                     /translation="MTEKRGKPDYDFAQEVLEVRDPEQFKAVGDATRQRIISLLTERA
                     ATTSQLAEALGQPKGSVGHHLKVLEKADLIRVVRTRQVRALTEKYYGRTARLFLFSDM
                     DESEASEPFMFLRQAMNEYLAPESGEEFGGSSTLAHARIPPERAQEFANRVWELAEEF
                     RSLEGVPGERVYGFLAQVYLTDLPEIPSEEEE"
     gene            complement(2173..2361)
                     /locus_tag="VFJ72_15595"
     CDS             complement(2173..2361)
                     /locus_tag="VFJ72_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480928.1"
                     /translation="MFAGSDPEHLLGMISQRQEEMRVAAREEAEVRRARPLRGARLAA
                     FRLWRLHVMVWFEDARRA"
     gene            complement(2496..2894)
                     /locus_tag="VFJ72_15600"
     CDS             complement(2496..2894)
                     /locus_tag="VFJ72_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480929.1"
                     /translation="MDLTHEYIHHRRGYRFGVGFCWIRIYGGEPGDAPVIVCEELPDL
                     GDMAKMSGYLAAEVIVEHFANGLPDLPRPMLWIEHRPRRRGPGRYFLLTFPGYAPHPE
                     GAGFVRRVTLGQPLREPLTPEEVAVLTGES"
     gene            complement(2912..3535)
                     /locus_tag="VFJ72_15605"
     CDS             complement(2912..3535)
                     /locus_tag="VFJ72_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET7480930.1"
                     /translation="MERRPELVVPYVTEQSPRGERTMDIYSRLLADRIIFLGTPVDDQ
                     VANVIMAQLLHLDSEDPEQDINLYINSPGGSVYAGLAIYDTMNFINADVATTALGMAA
                     SMGSFLLAAGARGKRSALPNTRILLHQPSIGGGIGGQVSDVEIHARELVRTKRRMNEL
                     YAEMTGQPMEKIEHDTDRDYMLSAGDAVEYGVIDRVVSRPGETVGAE"
     gene            3625..3894
                     /locus_tag="VFJ72_15610"
     CDS             3625..3894
                     /locus_tag="VFJ72_15610"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7480931.1"
                     /translation="MDLPHLRSFRQRAVMSQEQLAAKCGVARDTISKLESGKRRAYPT
                     TIRKLATGLEVEPQLLMGGVEYLDDAPSDEPVEERTENKGGKIGF"
     gene            complement(3895..4812)
                     /locus_tag="VFJ72_15615"
     CDS             complement(3895..4812)
                     /locus_tag="VFJ72_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480932.1"
                     /translation="MDLNELGLILASTIRNATPLIFAALGGMFSERSGVVNIGLEGLM
                     IIGAFAGVVGAFFSGSAVIGLATAVAAGLIFALIHAVMCITFEADQIISGTAINLLAL
                     GGTGFLMVVIFGSGGTSPKVESFKPIAIPGLSDIPLIGQAFFNQSILVYLMYLLVPVT
                     LFVVFRTPFGLRLRATGEVPEAVDTAGVSVTRMRYYGVALSGILASFGGVYLSMSLLS
                     AFTEGMTNGRGFIALAALVFGRWNPLGAAGAALLFGFAQAITFRVPQDVIPNEFVHML
                     PYILTIVVLAGFGGRAIAPAAIGKPYRKE"
     gene            complement(4812..5939)
                     /locus_tag="VFJ72_15620"
     CDS             complement(4812..5939)
                     /locus_tag="VFJ72_15620"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7480933.1"
                     /translation="MTEERREGRGSGGTSTARRLLGNLGGALLFPVFAILISFVIGAV
                     IILATGNNPVAAYAALVNGAFGNPAAIGRTLINTTPLVFTGLAVAFAFRAGLFNIGGE
                     GQVYIGALTAAWLGVSLGFLGPLAIPIVLAACVLTGFLWGAIPGVLKAYFGAHEVITT
                     IMLNFIAIDLTVWLALNPLRSKGLIPGTADIDAAARIPLLGLGLGRANFGIFIALLAA
                     VVAYLLLWRTRRGFQIRAVGLSPGAANYAGIGIGVNTILALAIGGAFAGLGGGVEVMG
                     IYGKMAIPFVSNLGFNGIGVALLGRNHPVGVVLGALLFGALASGAQQMQFDTNVPLDL
                     TSVLLAIILLFVTATKILEFVLGKRARELAAGTRLEKGLGS"
     gene            complement(5936..7528)
                     /locus_tag="VFJ72_15625"
     CDS             complement(5936..7528)
                     /locus_tag="VFJ72_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7480934.1"
                     /translation="MAEPRADGGTTGATETPVVLQMRNVTKTFGPVTANDDISLTLRR
                     GEILGLLGENGAGKSTLMKILYGLYHPDSGTIVVDGEEVNIRDPKDAVSRGIGMVHQH
                     FTLIPPLTVAENIVLGAEPRRRGTLDMNRAIRETEQLCERYGLRVDPRARVADLSVGV
                     QQRVEILKTLYRDARILVLDEPTAVLTPQETEDLFSVLKELVADGLSIILITHKLGEL
                     LGVSDEITIIRDGKVVDTVKTAGTDEGALARLMVGREVLLRVEKTESEVGEARLTAED
                     LVVHSSTGAVAVDGVSLEVRAGEILGIAGVDGNGQVELAEALAGTRPVHSGTVHLDGE
                     DVTRLGADARQERGLAYVPEDRSHKGLVQDFALYENNALKTFDEPPFSKGLFILPRAM
                     RRKAAENLKAYDVRPPDPDARAGSLSGGNQQKAILARELSGDPNVIIAAQPTRGVDVG
                     AIEFIHRQLLGQRSQGKAILLISLELEEIRSLSDRILVIYAGKIVGALDAADATDEKL
                     GLLMAGRDVPSENGSGRTEDRP"
     gene            complement(7538..8584)
                     /locus_tag="VFJ72_15630"
     CDS             complement(7538..8584)
                     /locus_tag="VFJ72_15630"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7480935.1"
                     /translation="MFGVCISVLALALVLAGCGGSGGGGNSGGGSANSNVKPGLVLDV
                     GGLGDQGFNDSAYAGMKRADKDLGVKGKYLESSAPTDYANNLTQLAENQADPIIAVGF
                     LMTDDVTEISKQFPDTQFAIVDSVVDTKNTISLVFREQEGSYLAGVVAGLMTQEKTPY
                     TNPDDKVVGFVGGQEGPLIGKFQAGYEAGVKSVCSDCKVLVQYAGSTPDAFNDPAKGK
                     EISQQQIEQGADVIYHAAGNTGRGLFEAAGEEKIFAIGVDLDQAKLFKDAPILTSVIK
                     RVDVAVEDTIKGVAKGGKPKGEIVDRGLKEKGLSLAPFGRFDKYVPQKVKDDVAKAKK
                     GIIDGKIKVPDKVQ"
     gene            8725..>9509
                     /locus_tag="VFJ72_15635"
     CDS             8725..>9509
                     /locus_tag="VFJ72_15635"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7480936.1"
                     /translation="MNPKNEPNTNFERDLAWMRAFTPATRASASALSSLEGERVLTVC
                     HLDLKMIPYFEALIDKGAEVWACAANPATTRNEVAERLNSIGVHVPARKDDPPGLHAD
                     HLSAAIEAAPTLLSEMGADATAATGGRLESVRGGLEATGTGIARIRDLDLSYPVFNWD
                     GVPIKQGLHNRHLVGLMAANTFLNVAALSLYGRTVLVVGYGPVGRGVADAARSFGATV
                     EVCDLDPSARLAAAHLGFATPGLEDGLSRADVVFTATGRDGAIP"
BASE COUNT         1716 a         3254 c         2904 g         1635 t
ORIGIN      
        1 atgcagcgca ccctctcgca accgcggcgc cggaccttct cgaagaacgt ttcgtacagt
       61 cgccgcccga gtccctcgtt cctgcaccct gggtccacac cggcgaagtg tacgtaagcc
      121 tctcttgcgg gggattggga gacgaacccg acgacgaaag cgacgatctc catcctcctc
      181 caccacgaag ctcgtgtcct ggaagtggtc gaagaatagc ctgggcagct tgccggctac
      241 attcctgccg tcccaccact cgttgacgac ggcgataatt ggtacgtggt ccgtctcccg
      301 gagcgctcta actatctatc aagcgttctc ctctcatcta cttcttgacc aggacatttt
      361 acgctcggga attggcgttt cggcgggctt cggagaccgt ccggttgttc acgaacggca
      421 ggaaggccag agcggctaag atcatcacgg cggcggagaa ccagaagggt gcggagaggc
      481 cgaagtgttt ggcgaggagg ccgccgatca aggctccgaa tgcgccgctg ccgacgcccg
      541 ccagccggta gacactcgtt acccggccca gcaggcgatc cggtatcaag gtctggcgta
      601 gagcgaaggt gaggacattc caggcgaaag ccgcgaagcc gccaaccgcc catatcagcg
      661 ccacgaccac gggattgccg ctcaacgcga tcccggcgaa cattggtccc tggataagca
      721 tgaccaggaa gagcgcccgt cccgtaccga tccatccgcc aacacgcccg gcgagcagcg
      781 agccgaagaa gcctccgacg ccaccggcgg tcatcaagac tccgtacccg aagctaccga
      841 ggccgagagt gtcctgggcg aagagcacca tgacggagaa agtagccgaa aatgtgaggt
      901 tcccggcccc cagcatgacc gccagggcga gcaacaaccg atgacgcgcc agccacccga
      961 tcccttccgc tatctcgacc aagatgccgg ccttctgtac gccgtctggt ctaacaaccc
     1021 gaaaccggcc ccggagagcc aggatcaaag cggaagaagc ggcgaaggtc cccgcgtcga
     1081 tgaggaacgg aaatgccgcg gcaaccgcga agagcgctcc tccaacggga ccgcccgcga
     1141 actggttcgt caccagctcc gcggcgaaca tctgaccatt ggccttctcc agttgctcct
     1201 tttgcaccat ggaaggcatg atcgacaacg aagcgttagc gaagagagtc tccgccgttc
     1261 ccaacaggaa gaagactccg tacatcagag gcaggctcgc ccagccggtg agcacggcga
     1321 gccccaggat tcctataaca ccagttcgga aggcgtcaac gctcgccatc aagaggcggc
     1381 ggtcgagcct gtccactagc gcgccgctga ggagcgagaa aagcaaccac ggaagctgct
     1441 gggcgaacgt caacccggcg accaaaaccg ggtctcttgt gaggctagcg gcgagcagcg
     1501 ggccagcgac cagcgtgatg ccgtcgccca ggttggagat cgtgctcgcg gtccagagct
     1561 tgcggtactc cgggccgaga ccgagccgga gcaaacgcta ttcctcttcc tctgagggga
     1621 tctccggtag atctgtcagg tagacctgcg ccaggaaacc gtagaccctc tcacccggca
     1681 cgccttcgag gcttctgaac tcctcggcca gctcccacac tcggttggcg aactcctggg
     1741 ctctctccgg cgggatgcgg gcgtgggcga gcgtggaact tccgccgaac tcctcccctg
     1801 actctggcgc caggtattcg ttcatggcct gccttaagaa catgaacggc tcggatgcct
     1861 cagactcatc catatcggag aacagaaaga gacgagccgt ccggccgtag tatttctccg
     1921 tgagcgcgcg cacctgccgc gtgcggacca ctcgaatgag atccgctttt tcgagcacct
     1981 tgagatgatg cccaacgctt cccttcggct gacctagcgc ctcggcgagc tggctggtcg
     2041 tcgccgctcg ctcggttagg agactgataa tcctctgcct cgtggcgtcc ccgaccgcct
     2101 tgaactgctc gggatctctt acctccagca cctcttgcgc gaagtcgtaa tccggtttcc
     2161 ctcgcttctc cgtcatgcgc gccgcgcgtc ctcgaaccac accatcacgt gcagccgcca
     2221 caacctgaac gcggcgagtc tcgctccccg gagaggacgc gcccgccgga cctcggcctc
     2281 ctcgcgcgcc gcaactctca tctcctcttg ccgctgcgag atcatcccca agagatgctc
     2341 gggatcagag ccagcgaaca ctcttcgtcc tttcggtcgt tatacgccaa acgttcgtca
     2401 tttaccgtat tgtcgaatat tgccgaccat aaatcaagca tcgatcttgc cgggccgaag
     2461 catgagccag ctatggtaaa aaggtcgcgc ggcctctagc tctctcccgt cagcacggct
     2521 acctcttccg gcgtaagggg ttcgcgtaat ggttgaccga gcgttacgcg tcgcacgaag
     2581 ccagcgcctt cgggatgggg cgcgtagccg ggaaaggtca gcaggaagta tcggcccggc
     2641 cctcgccgac gggggcggtg ctcgatccag agcatggggc gcgggaggtc cggcagcccg
     2701 ttcgcgaagt gttccacgat gacctcggca gccagatacc cggacatctt cgccatgtca
     2761 ccgagatccg gtagctcctc acacacgatt acgggagcgt cgccgggctc gcccccgtag
     2821 atccggatcc agcagaaccc caccccgaaa cggtagcccc gcctgtggtg aatgtactcg
     2881 tgcgtgaggt ccatcccgga ccgcttttct cctactctgc tcccaccgtc tcccccggac
     2941 gactcaccac ccggtctatg acaccgtatt ctacggcgtc cccggcgctg agcatatagt
     3001 cccggtcggt gtcgtgctct atcttctcca tcggttgacc cgtcatctcg gcgtacaact
     3061 cgttcatccg ccgcttggtc cgcacgagct ctctcgcgtg gatctccacg tcagagacct
     3121 gcccgccgat cccccctcca atgctcggct ggtgcagcag tattcgcgtg ttcggcagcg
     3181 cgctccgctt gcccctcgcg cccgccgcca gcaggaagga tcccatcgag gcagccatcc
     3241 cgagcgccgt cgtcgcaacg tcggcgttga tgaagttcat cgtgtcgtag atcgccagcc
     3301 ccgcgtacac gctcccgccc ggagagttta tgtacaggtt gatgtcctgc tccgggtcct
     3361 cactatctaa atgaagaagt tgcgccatga tgacgttcgc tacctgatcg tccaccggcg
     3421 tccccaggaa gatgatccta tccgccagaa gccgagagta aatatccatc gtccgctccc
     3481 cccgcggact ctgctccgta acatagggga ccaccaactc cggcctgcgc tccatcgcca
     3541 ctccttccag tgagctatac ttccagtaga gtagcattgt tgtagcgttt atgcaagttt
     3601 tatgtagcgt ttggaggtaa gggcatggat cttccgcatc tacggagctt ccggcagcgg
     3661 gcggtaatga gccaggagca gttggcggcg aaatgcgggg tggcgcgcga caccatctcc
     3721 aagctggaga gtggcaagcg ccgcgcgtat ccgaccacca tccgcaagct cgccaccggc
     3781 ctggaggtag agccgcaact gctcatgggc ggcgtcgagt acttggatga cgcgccctct
     3841 gatgagccag tcgaggagcg cacggagaac aagggcggga agatcgggtt ctagctattc
     3901 cttgcggtac ggcttgccga tggcggcggg ggcgatggcg cggccgccga agccggcgag
     3961 gacgacgatg gtgaggatgt aggggagcat gtggacgaac tcgttcggga tcacgtcctg
     4021 cggcacgcgg aaggttattg cctgggcgaa gccgaagagc agggccgcac cggccgcgcc
     4081 gagcgggttc caacggccga agacgagggc ggcgagcgcg atgaagccgc ggccgttggt
     4141 catcccctcg gtaaacgcgg agagcaggct catcgagagg tacacgccgc cgaaggaggc
     4201 caggatgccg gagagcgcca cgccgtagta gcgcatcctc gttacgctca cgcccgccgt
     4261 gtccaccgcc tcggggacct cgccggtggc gcgcaggcgc aggccgaacg gggtgcggaa
     4321 gacgacgaag agggttacgg ggacgaggag gtacatcagg tagacgagga tgctctggtt
     4381 gaagaacgcc tgccctatca gggggatgtc gctcaggccg ggaatggcga tgggcttgaa
     4441 gctctcgacc ttcggggagg ttccgccgga accgaagatc acgaccatca ggaagcccgt
     4501 gccgccgagg gccaggaggt ttatggcggt gccggagatg atctggtccg cctcgaatgt
     4561 tatgcacatg acggcgtgga tcagggcgaa gatgagtccc gccgccaccg ccgtcgccag
     4621 gccgatgacc gcgctcccgg agaagaacgc tccaactacc ccggcgaagg cgccgatgat
     4681 catgagccct tcgagtccga tgttcaccac cccgctccgc tcgctgaaca tgcccccgag
     4741 ggccgcgaag atcagcggcg tcgcgtttcg gatggtcgag gccaggatca gtccaagctc
     4801 gttgaggtcc attacgagcc cagtcccttc tccagccggg tccccgcggc cagctcgcgg
     4861 gcgcgcttgc cgaggacgaa ctccaggatc ttggtcgccg tcacgaagag gaggattatg
     4921 gcgagcagaa cgcttgtcag gtcgagcggg acgttggtgt cgaactgcat ctgctgcgcc
     4981 cccgaggcga gcgccccgaa gagcagcgcc cccagcacca ccccaaccgg atggttgcgt
     5041 ccgagcaacg ccaccccgat gccgttgaag cccaggttcg agacgaacgg gatcgccatc
     5101 ttgccgtaga tccccatcac ctccacgcct ccgccgagcc ccgcgaaggc cccgccgatg
     5161 gcgagcgcca ggatagtgtt caccccgatc ccgatccccg cgtagttcgc cgcccccgga
     5221 gagagcccga ccgcccggat ctggaacccc cgccgcgtcc gccacagcag caggtaagcc
     5281 accaccgccg ccaaaagcgc gatgaagatg ccgaagttcg cccgccccag acccagcccg
     5341 agcaggggta tgcgggccgc ggcatctatg tccgccgtgc cggggatgag ccctttagag
     5401 cgcaacgggt tcagcgcgag ccacaccgtg aggtcgatag ctatgaagtt cagcatgatc
     5461 gtcgtgatga cctcgtgcgc cccgaagtat gccttcaaga cccccggtat cgctccccac
     5521 aggaaacccg tcaacacgca ggcggcgagc acgatcggta tcgccagcgg tccgagaaac
     5581 ccgagagaca cccccagcca ggccgccgta agcgcaccga tgtagacttg cccctccccc
     5641 ccgatgttga agagcccagc ccggaacgcg aacgccacgg ccagccccgt gaagacgagc
     5701 ggtgtagtgt tgattagcgt ccggccgatg gccgccgggt tcccgaacgc gccgttcacc
     5761 agagccgcgt aggcggcgac cgggttgttc cccgtggcga ggattatgac tgctcctata
     5821 acgaaggaga tgaggatggc gaagaccggg aagagcagcg cgcccccgag gttgcccaac
     5881 aaacgtcgcg ccgtcgaggt cccgccggag cctcggccct cccggcgctc ctcggtcacg
     5941 gacggtcctc tgtcctgccg ctgccgtttt cagaaggaac gtcgcgcccg gccatgagga
     6001 ggccgagctt ctcatcggtg gcgtcggcgg cgtccaacgc gcccacgatc tttccggcgt
     6061 agatcaccag tatgcggtcg gagagcgagc gaatttcctc cagctccagc gagatgagga
     6121 gtatcgcctt gccctgcgac cgctgcccca gtagctgccg gtggatgaac tctatggccc
     6181 ccacgtccac gccgcgggtc ggctgcgccg cgatgatgac gttcgggtcg ccggagagct
     6241 cgcgcgccag gatggccttc tgctggttcc cgccggagag agatcccgcc ctcgcgtccg
     6301 ggtccggcgg tcgcacgtcg tacgccttga ggttctcggc ggccttgcgg cgcatcgctc
     6361 gcggcaggat gaagagcccc ttggagaacg gcggttcgtc gaaggtcttg agggcgttgt
     6421 tctcgtagag cgcgaagtcc tgcacgagac ccttgtgcga gcggtcctcc ggcacgtagg
     6481 ccagaccgcg ctcctgccgc gcgtcggccc cgagcctcgt aacgtcttca ccgtcgaggt
     6541 ggacggtccc gctgtgtacc gggcgcgtcc cggccagcgc ctcggccagc tccacctgcc
     6601 cgttaccgtc cacgccggcg atgcccagga tctcgccggc ccgcacctca aggctcaccc
     6661 cgtccaccgc caccgcgccg gtcgaggaat gaacgaccag gtcttcggcg gtcagccgcg
     6721 cctccccaac ctcggactcg gttttctcga cgcgcaacag cacctcgcgc ccgaccatca
     6781 gccgggccag ggcgccctcg tccgtcccgg cggtctttac cgtgtcaacg actttgccat
     6841 cgcggattat ggtgatctca tcggaaacgc ccagcagctc gcccagcttg tgcgtgatca
     6901 ggataataga gagaccatcg gcgaccagtt ccttgaggac ggagaatagg tcttccgtct
     6961 cctggggagt gaggacggcg gtcggttcgt cgaggaccag gatgcgggcg tcgcggtaga
     7021 gggtcttgag gatctcgacc cgctgctgca cgccgaccga gaggtccgcg accctggcgc
     7081 gcgggtcaac ccgcaggccg tagcgctcgc agagctgctc cgtctcgcgg atggcgcggt
     7141 tcatgtccag cgtcccgcgc ctgcggggct ccgcgcccag tacgatgttc tcggcgacgg
     7201 tgagcggtgg gatcagggtg aagtgctgat ggaccatgcc gataccgcgc gagacggcgt
     7261 ccttcgggtc ccttatgttc acctcctcgc cgtccaccac gatggtgccc gagtccgggt
     7321 ggtagagccc gtagaggatc ttcatcaggg tggacttccc ggcgccgttc tcgccgagga
     7381 ggcccaggat ctcgccgcgg cgcagcgtga gggagatgtc gtcgttggcc gtgacgggac
     7441 cgaacgtctt ggtgacgtta cgcatctgga ggactaccgg ggtctcggtg gccccggtag
     7501 tcccgccgtc tgcccgcggc tcggccaagt tggttaccta ctgcaccttg tcggggacct
     7561 tgatcttgcc gtctatgatg cccttcttgg ccttggccac gtcgtccttg accttctgcg
     7621 gcacgtactt gtcgaagcgt ccgaacggcg cgagagagag gcccttctcc ttcaggccgc
     7681 ggtccacgat ctcgcccttc ggcttgccgc ccttggcgac gcccttgatg gtgtcctcga
     7741 cggccacgtc cacccgcttg atgacgctcg tcaggatcgg cgcgtccttg aagagcttcg
     7801 cctggtcgag gtcgacgccg atggcgaaga tcttctcctc gcccgccgcc tcgaagagac
     7861 cgcgcccggt gttgccggcg gcgtggtaga taacgtccgc gccctgctcg atctgctgct
     7921 gggagatctc cttgcccttg gccgggtcgt tgaaggcgtc cggcgtcgaa ccggcatact
     7981 ggacgagtac cttgcagtcg gagcagacag acttgactcc ggcctcgtag ccggcctgga
     8041 acttgccgat caacggccct tcctgcccgc cgacgaaccc gaccaccttg tcgtcggggt
     8101 tggtgtacgg ggtcttctcc tgggtcatca ggcccgccac cacgcccgcg aggtagctgc
     8161 cctcttgctc ccggaagacg aggcttatgg tgttcttcgt atccacgacc gagtccacga
     8221 tcgcgaactg cgtgtcgggg aactgcttgg agatctcggt gacatcatcg gtcatgagga
     8281 acccgacggc gatgatcgga tcggcctggt tctccgcgag ctgggtgagg ttgttggcgt
     8341 agtcggtcgg cgcgctagac tccaggtact tgcccttgac gcccaggtcc ttgtccgccc
     8401 gcttcatgcc cgcgtaggcg gagtcgttga acccctggtc accaagccct ccgacgtcca
     8461 gcacgagccc cggcttgacg ttgctgttgg ccgaaccgcc gccggaatta cccccgccac
     8521 cggagccgcc gcatccggcc aggaccagcg ccagagctag aacggaaata cagactccga
     8581 acaacctcga taccttcata tgcctccctc gcagtaggac ctcgcacgat aaacgcccgg
     8641 cgggctcgtt cccccggaac ctgccccgat tgcagggatg atatagagta atgagcctcg
     8701 acgcaaaggg tagagttccc atccatgaac cccaagaacg agccaaacac aaacttcgag
     8761 agagacctcg cctggatgcg cgccttcacc cccgccacca gggcctccgc ctccgcccta
     8821 tcttctttgg aaggtgaacg agtcctcacc gtctgccacc tggacctcaa gatgatcccc
     8881 tacttcgaag ccctgataga taaaggagcc gaagtctggg cctgcgccgc aaaccccgcc
     8941 acgacccgca acgaggtcgc cgagcgcctg aactcgatag gcgtacacgt cccagcccgc
     9001 aaggacgacc caccagggct acacgccgac cacctgagcg ctgctatcga agccgccccc
     9061 accctactct ccgagatggg cgcggacgcc accgccgcca ccgggggtcg tctggagagc
     9121 gtccgcggtg gtctcgaagc caccggaacc ggcatcgccc gcatccggga cctggacctc
     9181 tcctaccctg tctttaactg ggacggcgtc cccataaagc agggtctcca caaccgtcac
     9241 ctcgtcggcc tcatggccgc caacaccttc ctcaacgtcg ctgccctctc cctctacggc
     9301 aggacggtcc tcgtggtcgg ctacggtccc gtcggccggg gcgtcgccga cgccgcccgc
     9361 tccttcggcg cgaccgtgga ggtgtgcgac ctcgacccct ccgcccggct cgccgccgcc
     9421 cacctcggct tcgccacccc aggactcgaa gacggcctct cccgcgccga cgtggtattc
     9481 accgccaccg gccgcgacgg cgcgatccc
//
LOCUS       DASSMB010000275         7616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_990249, whole genome shotgun sequence.
ACCESSION   DASSMB010000275 DASSMB010000000
VERSION     DASSMB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7616
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990249"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="VFJ72_15640"
     CDS             complement(<1..1240)
                     /locus_tag="VFJ72_15640"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7480937.1"
                     /translation="MSDFPNGSPENGGDAHAGPGAGKAEEALRARVRQQEATGMLSLS
                     ALSGTGLPDLLNLVAETVASVLSVEFCEVLELLPDGDRLLLRAGVGWREGRVGEATVG
                     TNLESQAGYTLISRGPVVVEDLRTEGRFRGPPLLREHGAVSGISTTIRIGEHFYGVIG
                     AHTARKRSFTDDEVLFLQEVADVVGAAIERLRDESDVRELLGERTELAAVAERRFEFL
                     TEANALLSTSTDYGTVLATAVRLAVPAVADWCFVDVVEDSGGAVERFTVARSEPGDES
                     LTRELQSRYPLDPNTQHGTPGVLRTGRPELIPDVDDAVIRETVRDRANLEMLQSLDPK
                     SYMCVPLRVGGRTLGAMGFVSSDAGRRYGEEDLALAEGLAYSAAVAIDNARHHVSEAE
                     LARELVQRARQDQRTAAPLGR"
     gene            complement(1330..1743)
                     /locus_tag="VFJ72_15645"
     CDS             complement(1330..1743)
                     /locus_tag="VFJ72_15645"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HET7480938.1"
                     /translation="MSEGGIRAGIVALAVAGLMISAYLTWTHFQGTTPVCVGGGSGCE
                     TVQTSRYSVILGVPVAVLGLFAYTGLLVSAAVRGGNAALLGLFLALVGVLFSAYLTWL
                     EIFVIRAICQWCVASAVLITGALLLAFVRVRRAGN"
     gene            complement(1740..2399)
                     /locus_tag="VFJ72_15650"
     CDS             complement(1740..2399)
                     /locus_tag="VFJ72_15650"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7480939.1"
                     /translation="MQQGNMRLVAALVVVAVAVAGVMVLLSQIGGGGSSEDLAGIPQD
                     GTTLGEKDAPVMIKLYEDFQCPACGMFARDTLPQVIEDYVKSGKVKLVSETLTFIGPD
                     SVPAARAALAAGEQDRYWQYASLLFENQGAENSGYVTEKFLNSLADQTKGLDVEEWNA
                     ARKGSSTEEELKAVQERAVKDKVQATPTLVISGPGGERTLRGAVPVDEVGEAVEKVRG
                     S"
     gene            complement(2446..2784)
                     /locus_tag="VFJ72_15655"
     CDS             complement(2446..2784)
                     /locus_tag="VFJ72_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480940.1"
                     /translation="MKSDTISGMVSKARRPRLFLLAALLAAFIFFLPYLEATGSCGDP
                     GCPQISQAHGPVSGELAAAAIAAILAAAPATPVLAAALIGPRPASDRTPAQVYLSPDP
                     GPPRLRTGGL"
     gene            3007..3612
                     /locus_tag="VFJ72_15660"
     CDS             3007..3612
                     /locus_tag="VFJ72_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET7480941.1"
                     /translation="MMRRFTLILLAIFLASGLAWYVQPGTEPASAATVRKPDQILGAP
                     RYSQKKVERYARSIGCTRYIMKTIPIYYKLAPKRNIAPDVLVAQAILETGKGYYGGDS
                     RPWNMAGIKKGGIVGDSPEDFERPATAYEGVRMHVNHMAAYTGKKPIGKPHDRYYDAR
                     SAQKSRGWWVRKVSQLGGGVWATDTTYGGKIRNILDAMGRQ"
     gene            3827..4222
                     /locus_tag="VFJ72_15665"
     CDS             3827..4222
                     /locus_tag="VFJ72_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480942.1"
                     /translation="MASEETKLDTYSIDDVIKVPVSLKDEDGVAHVRVIFRRMRRPGG
                     IGPRGLDPEDTLELRGNGRGEVEATVETTLKVADGHKPGEYLCVAIQVYDARGNMVMI
                     KNPTPTRLFKIVDLGNKDDKKTTFLGWGD"
     gene            complement(4291..5493)
                     /locus_tag="VFJ72_15670"
     CDS             complement(4291..5493)
                     /locus_tag="VFJ72_15670"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET7480943.1"
                     /translation="MGAKITHVETAAIRAVGPSVLVKVWAGDEYGLGECYPSAPAAGI
                     HQIITNLEDQLVGEDPRNVEMLYEKLRRWNIFTGGQAGAVLTAISGIEMALWDLAGKL
                     QGVPIYRLLGGAFRTRIRLYADFNAGTVDAAAHHTTDGGLLSEELQQEYVDVAREAVS
                     MGFDAIKFDVDDVTGPLHRDFWNGAISGPEREAMVARVAAVREAVGPEVDLAIDMHGR
                     FDLPSSIRFARAVEEFDLLWLEEPTPPENYDALAEIRRSASTPICAGENTYTRFDFKR
                     LFDGRCVDYVMPDVAKCGGLAEGKRIANMAELDYIPFATHNVSSPIGTIAAAHVCAAV
                     SNFAVLEWHAIDMPHWADFVRYSGGDVISDGHIELTEAPGLGLELNEEAAFEHRHDKG
                     GMPFFERG"
     gene            5821..6267
                     /locus_tag="VFJ72_15675"
     CDS             5821..6267
                     /locus_tag="VFJ72_15675"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7480944.1"
                     /translation="MKTTGFTHVSVSARDLEESARFYKDIFGMEEVPSPDFPFPVRWL
                     RVGDLQLHLFRSDDPAPAAHHFGVEVDDFEAAYKKVNELGVGIGDGYFSNVYELPDGA
                     VQLYIRDPAGNMLEINWPDASSLDRSVVPETQKVPGSRDARLYMRR"
     gene            6329..6814
                     /locus_tag="VFJ72_15680"
     CDS             6329..6814
                     /locus_tag="VFJ72_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HET7480945.1"
                     /translation="MAEDLGMRIVEEMGLPDQAIDSDELPWVPQGERVWFKPLRFDLS
                     SGRWINLLKVEGSGKVNRHRHSGGQVMGYCIEGSWHYLEREWVARPGTLVYEPPGDIH
                     TLVVDEGVERMITLFILEGTVQYLDDNDGLIYQDDVFSKLERYLNFCEQQGIEPQDLR
                     Y"
     gene            6814..7581
                     /gene="kduD"
                     /locus_tag="VFJ72_15685"
     CDS             6814..7581
                     /gene="kduD"
                     /locus_tag="VFJ72_15685"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008678 - 2-deoxy-D-gluconate
                     3-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HET7480946.1"
                     /translation="MSILDAFDLSGKVALVTGSSRGLGQGISLGLAEAGADIAALDIV
                     PVTETGEQVSALGQRFHPINFDLLETKAEQAAKIVGECVEELGRLDILVNNAGIIRRA
                     PALEFGERDWEDVIAINLSAAFYLSQAAARHFVERGGGKIINTASVLSYEGGITVPSY
                     SAAKAGIANLTRALANEWASLGINVNAIAPSYFTTELTSALREDTERSEALLARLPAG
                     RYGEPEDLKGAVVFLASDAASYVHGAILPVDGGWLGR"
BASE COUNT         1416 a         2548 c         2386 g         1266 t
ORIGIN      
        1 gtcggcccag gggagccgcc gtccgctgat cctgcctcgc gcgctggacc agttcccgcg
       61 ctagctccgc ttcggagacg tggtggcggg cgttgtctat ggcgacggcc gcgctgtagg
      121 ccaacccctc ggcgagcgcc aggtcttcct cgccgtaccg gcgcccggcg tcggaggaga
      181 cgaagcccat cgctcccagg gtgcggcccc cgacccgcag cggtacgcac atgtacgact
      241 tcgggtcgag gctctggagc atttccaggt tcgcccggtc gcggaccgtc tccctgataa
      301 cggcgtcgtc gacatccggg atcagctcgg gcctgccggt cctgaggacc cctggcgttc
      361 cgtgctgggt gttgggatcc agggggtagc gactctgaag ctcgcgcgtc aatgactcgt
      421 cgccgggctc ggaacgcgcg accgtgaagc gctcgacggc gcctccggag tcctcgacca
      481 cgtctacgaa gcaccagtcg gccaccgcgg gtacggcgag gcggaccgcc gtggcgagca
      541 cggtcccgta gtcggtcgag gtcgagagca gcgcgttggc ctccgtgagg aactcgaacc
      601 gccgctcggc cacagccgcc agctccgtgc gctcgcccag gagttcgcgg acgtcgctct
      661 cgtcccgcag gcgctcgatg gcggctccta ccacgtcggc cacctcctgc aggaacagca
      721 cctcgtcatc ggtgaacgag cgcttcctgg ccgtgtgcgc cccgatgaca ccatagaagt
      781 gttcgccgat gcggatcgtg gtgctgatac cgctcacggc gccatgctcc cgcaggagcg
      841 gcgggcctct aaagcggccc tcggtccgca ggtcctcgac cactactgga cccctggaga
      901 ttaacgtgta ccccgcctgc gactccagat tggtcccgac cgtggcttcg cccacgcggc
      961 cttctctcca accgactcct gcccgcagca agagccggtc cccatccggc agcaactcca
     1021 gcacctcgca gaactcaacg gagagtacgc tggcgacggt ttcagccacg aggttcagga
     1081 ggtccggcag gccggtcccc gagagcgccg agagactcag catccccgtc gcctcctgct
     1141 ggcgcacccg ggcccttagc gcctcttcgg ccttcccggc tccaggcccg gcatgtgcgt
     1201 ctccaccgtt ctccgggcta ccgttcggaa agtcgctcac accctacctg tccttccagc
     1261 ttgcgcccaa cacgcggcat gccacgcatc atatccaagc ctctcttctc ccgcagcggc
     1321 gtgggtccgt cagttccccg cccgcctcac ccggacgaag gcgaggagca gagcgcccgt
     1381 aatgaggacc gcgctcgcca cgcaccactg gcagatcgca cgaatcacga agatttcgag
     1441 ccacgtcaaa taggcgctga acagcacgcc gaccagggcg aggaagaggc cgagcagggc
     1501 cgcgttcccg ccccggacgg cggccgagac caagagaccc gtgtaggcga acaggcccag
     1561 cacagcgacg ggaaccccca ggatgaccga gtagcggctc gtctgcaccg tctcgcaccc
     1621 gctgccgcca cccacgcaga cgggagtcgt cccctgaaaa tgcgtccacg tcaggtaggc
     1681 gctgatcatg agccccgcca cggccagggc gacgatgccg gccctgatgc ccccctcgct
     1741 cacgaacctc tgaccttctc tacggcctcc ccgacctcgt ctaccggcac cgcgcccctg
     1801 agcgtccgct cgccgcccgg accggagata acaagcgtcg gggtggcctg gactttgtcc
     1861 ttcaccgccc tctcctgaac ggccttcagc tcctcctcgg tggagcttcc tttacgcgcg
     1921 gcgttccatt cttccacgtc gagtcccttt gtctgatccg ccaggctgtt caagaacttc
     1981 tccgtgacgt agccgctgtt ctccgctccc tggttctcga agagcaggga ggcatactgc
     2041 cagtagcggt cctgctctcc ggccgccagc gccgccctgg cggcggggac cgagtcggga
     2101 cctatgaagg tcagcgtctc ggagacgagc ttcactttcc cggactttac gtagtcttcg
     2161 atcacctgcg gcagcgtgtc gcgcgcgaac atgccgcaag ccggacactg aaagtcctcg
     2221 tagagcttta tcatgaccgg cgcgtccttc tcacccagcg tcgtaccatc ctgcgggatt
     2281 cccgccaggt cttcggaact gccgccaccg ccgatctgac tcaacaacac catcactccg
     2341 gccacggcga ccgccaccac gaccagcgcg gcgacgagcc gcatgtttcc ttgctgcaca
     2401 gccagcacct ctcttctccg atacctccag accgcgtagg ggcctttaga gcccgccagt
     2461 ccggagccgg ggcgggcctg ggtcgggaga gaggtatacc tgcgcgggcg tccggtcgga
     2521 ggcgggacgg ggcccgatca aagccgccgc gagaaccggg gtcgcgggcg cagccgccag
     2581 tattgccgct atagccgccg cggccaactc gccggaaaca ggtccatgag cctgcgagat
     2641 ctgcggacat ccgggatctc cacaggaacc cgtcgcttcc aggtaaggga ggaagaagat
     2701 aaaagcggcc agcaacgccg cgagcagaaa gaggcgggga cggcgcgctt tcgagaccat
     2761 gccagagatt gtatcgctct tcatcagagc tctctcgaaa accattctaa aagctggcgc
     2821 cgcggtcgga taccgcgcaa tgaatcaata cctccggctc tcgaatgcgc tattctgggc
     2881 cgcttccctc ttacggacgc tcctaggtca ttcacgggct ctaaagtttc atcgcccggc
     2941 gtccgatgat atcgtgtagg atactgaacg gatcgccgga ggcggcaact tcggctattg
     3001 agggggatga tgcggcgctt cacattgatc ttgctggcta tatttctcgc ttctggactg
     3061 gcctggtatg tccagccggg taccgaaccg gcctccgcgg cgacggtacg caaacccgac
     3121 cagatactcg gcgctccccg atacagccag aagaaggtgg agagatacgc ccgttcgata
     3181 ggatgcacgc gctacataat gaagaccatc ccgatctact acaagctggc cccgaagcgg
     3241 aacatagcgc cggacgtgct ggtcgcccag gccatcctgg agaccggcaa gggatactac
     3301 ggcggcgact cgcggccgtg gaacatggcc ggcatcaaga agggcggcat cgtgggcgat
     3361 agccccgaag acttcgagag accagccacc gcctacgagg gcgtgaggat gcacgttaac
     3421 cacatggccg cgtacacggg gaagaagcca atcggcaagc cgcacgaccg ctactacgac
     3481 gcccgctccg cgcagaagag ccgcggatgg tgggtaagga aagtcagcca gttgggcggc
     3541 ggcgtctggg ctaccgacac cacgtacgga ggtaagatcc gcaacatcct cgacgccatg
     3601 ggcaggcaat aacccctaag aacgtccgga gcgccgatcc gcgtcggcgc tcctctccac
     3661 gtaaaaccag acagagtgcg gagctcgcca cagaggccgc gcaagtcctg ttgggcagcc
     3721 ccgtctccga accggaggcg agtagttgac aaggagaatc aggtcattaa gctctgtatt
     3781 atctctccgc gtagcagtgc agtagcttac ataacaggag gtgccaatgg cttccgagga
     3841 gaccaaactc gatacttact cgatagacga cgtaataaag gtccccgtct cgctgaaaga
     3901 cgaggacggc gtggctcacg tgagggtcat cttccggcgt atgcgccgcc ccggcggcat
     3961 cggacccagg ggtctcgacc ccgaagacac ccttgaactg cgcggcaacg ggagaggcga
     4021 agtcgaagcc acggtcgaaa cgacgctgaa agtcgcggac ggtcataagc cgggggagta
     4081 tctgtgcgtg gcgatacagg tctacgacgc caggggaaac atggtaatga tcaagaatcc
     4141 cacgcctacc aggctcttca agatcgtgga cctgggcaat aaggacgata agaagactac
     4201 gttcctcggg tggggagatt agatactcag gctcctgata ctggcggctc gcaaggggcg
     4261 ggtggcgcat cgccctttcg agttttcgtg ctaccctcgc tcgaagaacg gcatcccgcc
     4321 cttgtcgtgg cggtgctcga acgcggcttc ctcgttcagc tccaggccga gaccgggagc
     4381 ctctgttagc tcgatgtgac cgtccgagat cacgtcgccg cccgagtagc ggacgaagtc
     4441 ggcccagtga ggcatatcta tggcgtgcca ctccaggacg gcgaagttgg agacggcggc
     4501 gcagacgtgg gcggctgcga tggtccctat ggggctggat acgttgtgcg tggcgaaggg
     4561 gatgtagtcc agctcggcca tgttggcgat gcgcttgccc tcggcgagcc cgccgcactt
     4621 ggcgacatcg ggcatcacgt agtcaacgca ccggccgtcg aagagccgct tgaagtcgaa
     4681 gcgggtgtag gtgttctccc cggcgcagat cggagtggag gcgctgcggc ggatctcggc
     4741 cagcgcgtcg tagttctccg ggggcgtagg ctcctccagc cacagtagat cgaactcctc
     4801 caccgcccgc gcgaaacgga tagaagaggg taggtcgaag cgcccgtgca tgtctatagc
     4861 gaggtccacc tccgggccga ccgcctcccg gacagccgcc acgcgcgcaa ccatcgcctc
     4921 ccgctccggc ccggagatag cgccgttcca gaagtcccgg tgcagcggtc cggtaacatc
     4981 gtccacgtcg aactttatgg cgtcgaagcc cattgagaca gcctccctcg ccacgtccac
     5041 gtactcctgc tgcagttcct ccgagagcag gccgccgtct gtggtgtggt gggccgccgc
     5101 gtcaaccgtg cccgcgttga agtcggcgta gagccgtatt ctggtccgga aagcgcctcc
     5161 cagcagccgg tagatgggca cgccctggag cttccctgcc aggtcccaga gcgccatctc
     5221 gatgccggag atagccgtga gaaccgcccc ggcctgcccg ccggtgaaga tgttccagcg
     5281 ccggagcttc tcgtagagca tctccacgtt gcgcgggtct tccccgacga gctggtcttc
     5341 caggttcgtg atgatctggt gtatgcccgc cgccggcgcg gatgggtaac attcgccgag
     5401 gccgtactcg tcccccgccc agaccttcac cagcaccgac ggcccgaccg cccgaatagc
     5461 cgccgtctcg acgtgcgtga tcttcgcgcc catccagccc tcccgctcgg taataggttt
     5521 ccgcattgcg tgcgcccgga cgccggggga gttcacgtct caacccaggc ccgctcctgc
     5581 cacgcggcga acggcctata ccggcgatgg cgcgaggatc gcgctaacat ccccgccgtt
     5641 ccgcgcaacc ggcatctagc ggcgctgccg gggaacattc aaaatacaga ctctgacaaa
     5701 gaaaccttcc aggagaaaga ccatgatcga ggaccagata gagccgctca tccagacgcc
     5761 gttctactcc accagcgaga ccgccgagat ccgggcggag atccgaggac agtttaggag
     5821 atgaagacta ccggctttac gcacgtaagc gtcagcgccc gcgacctgga agaatcggcg
     5881 cgtttctaca aggacatctt cgggatggag gaggtgccat cgccggactt cccgttcccg
     5941 gtgcggtggc tacgggtggg cgacctgcaa ctgcacctct tccggagcga cgaccccgcg
     6001 cccgccgccc accacttcgg cgtcgaggtg gacgatttcg aggccgcgta caagaaggtg
     6061 aacgagctgg gagttggaat cggagacgga tacttctcga acgtctacga gcttccggac
     6121 ggtgcggtgc aactatatat ccgcgacccg gcgggcaaca tgctggagat caactggccc
     6181 gacgccagtt cgctcgaccg ctcggtggtg ccggagacac agaaggtccc cggcagccgg
     6241 gacgcccggc tctacatgcg ccgctgaact gttgctcgcg gctcaccggg gcgttaggct
     6301 ggaggcaggc tagggaaagg ggttattcgt ggccgaggac ttggggatgc ggattgtcga
     6361 ggagatgggg ctaccggacc aggcgataga ctcggacgag ttgccgtggg tgccgcaggg
     6421 ggagcgggtg tggttcaagc cgctgcgctt cgacctgtcg agcgggcgtt ggatcaattt
     6481 gctcaaagtc gagggctcag ggaaggtcaa ccgccaccgg cactccggcg gtcaggtaat
     6541 gggttactgc atcgagggtt cgtggcatta tctggaacga gagtgggtgg cgcgtccggg
     6601 cacgctagtc tacgagccgc cgggagacat ccacacgctg gtcgtggacg aaggggtgga
     6661 gcgcatgatc acgctcttca tcctcgaagg gaccgtgcaa tacctcgacg ataacgacgg
     6721 cctgatctac caggacgacg tcttctcgaa gctggagcgc tacctgaact tctgcgaaca
     6781 gcagggcatc gaaccgcagg accttcggta ctagtgagca tcctcgacgc cttcgacctg
     6841 agcggcaagg tggccctcgt gaccggctcg tcccgtggcc tgggacaggg gatctcgctc
     6901 ggtctcgccg aagccggcgc cgacatcgcc gccctcgaca tcgtacccgt cacagagacc
     6961 ggcgagcagg tctctgccct cggacaacgc ttccacccca taaacttcga cctcctggag
     7021 acgaaggcgg agcaggcggc aaagatcgtc ggcgagtgcg tcgaggagtt ggggcgcctg
     7081 gacatcctgg tcaacaacgc cggcatcatc cgccgggcgc ccgcgctgga gttcggcgag
     7141 agggactggg aggacgtaat agcgatcaac ctgagcgccg cgttctacct ctcccaggcg
     7201 gcggcccggc acttcgtcga acgtggcggc ggcaagatca tcaataccgc ctcggtcctc
     7261 tcctacgagg gaggcataac cgtgccgtcc tactcggcgg cgaaggccgg catcgccaac
     7321 ctaacccgcg ccctggcgaa cgagtgggcc tcgctcggca tcaacgtcaa cgccatcgcc
     7381 ccgagctact tcaccaccga gctcacgagc gcgctgagag aagacacgga acgctccgaa
     7441 gctctactcg cccgcctccc cgccggacgc tacggcgaac ccgaggacct caagggagcg
     7501 gtggtcttcc tcgcctcgga cgccgcctcc tacgtccacg gcgcgatcct gccagtagat
     7561 ggcggttggc tgggacgcta acgcctgcat cgaaaccccg gcgttcttcg gtgtcc
//
LOCUS       DASSMB010000276         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_360651, whole genome shotgun sequence.
ACCESSION   DASSMB010000276 DASSMB010000000
VERSION     DASSMB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360651"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VFJ72_15690"
     CDS             complement(<1..571)
                     /locus_tag="VFJ72_15690"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET7480947.1"
                     /translation="MNEFEVIKQITDLLPGTPPEVLVPIGDDCAVLRLGDKDWVAAAD
                     MLVSGHHFKGWASPEDVGYKAVAVNVSDVAAMGGTPRFVLASGGAPDPEITLRCFRGV
                     AEACEAFGAYPIGGDTTRADALTVDISILGELSSPPALRSGARPGDLLAVTGELGASA
                     AGFLALEAGLDGPERLIRRHLRPKPRVEAG"
     gene            complement(568..774)
                     /locus_tag="VFJ72_15695"
     CDS             complement(568..774)
                     /locus_tag="VFJ72_15695"
                     /inference="COORDINATES: protein motif:HMM:NF033684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter suffix domain-containing protein"
                     /protein_id="HET7480948.1"
                     /translation="MIPAALTLSTLAVPFLPLPTEVKVWTVSGLLISTEVVFWGAALY
                     FGGEVAARYRRFFNPRNWFGKKRR"
     gene            complement(879..1748)
                     /locus_tag="VFJ72_15700"
     CDS             complement(879..1748)
                     /locus_tag="VFJ72_15700"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET7480949.1"
                     /translation="MRADLVSGILPVREDGRMLLLQRPAGTWEPPAGRLATGETFQEG
                     AVRELYEETGMLVAPQRILATWVGDSPNGGSLAAVTYIGRTDEEEVRLSHEHLGYKWV
                     TLDEWLELPSWWSKENIRGVAGPLRELPRDPSPEPEPPEPGRDHAVVPANLGAGAVLV
                     DFDGAEPRALLLRRRKPPVGLWENPGGMLEGGEDFVACARRELFEETGVEAEPVSPWW
                     ARVEPWRGPRDHDLYAGVGFLARHPGGGISPEHVAHDTFRWVTETEWRSLRTWYTNDE
                     SDVLWSKVREMGA"
     gene            complement(1817..2446)
                     /gene="thiE"
                     /locus_tag="VFJ72_15705"
     CDS             complement(1817..2446)
                     /gene="thiE"
                     /locus_tag="VFJ72_15705"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7480950.1"
                     /translation="MKPRERLRGTRLLLVTDPRPDLAERVAAGVRGGVDIVQLRDKRA
                     AREDLLPLAEELKEVCARGALFTVNDDVELARISAADGVHLGQEDGPLARARELLGPD
                     TIVGRSVGSVEEAREAVREGADYLGVGTIYGTPTKTDTEVAGVALLERISREDPPVPW
                     FAIGGVTLETAPEVAAAGAYGFAVVRAILDAEDPESAARELRSLLLGSK"
     gene            complement(2443..>2654)
                     /locus_tag="VFJ72_15710"
     CDS             complement(2443..>2654)
                     /locus_tag="VFJ72_15710"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HET7480951.1"
                     /translation="YALYATDRHGRLRRGDVHHVMWPLQPAEVEVEKLEMTGQIGLSL
                     PGADPVLHFARRLDVLAWPPERVGP"
BASE COUNT          404 a          989 c          868 g          393 t
ORIGIN      
        1 gcccggcctc cacccgcggc ttcgggcgca ggtgcctgcg gatgaggcgc tcaggaccgt
       61 cgagcccggc ctccagagcc aggaacccgg cggccgacgc tcccagctcg ccggtgacgg
      121 ccaggaggtc gccgggccgc gccccggagc ggagcgcggg cggcgacgat agctcgccca
      181 gtatcgagat gtccaccgtg agcgcgtcgg cgcgggtggt gtcgcctcct atcggataag
      241 cgccgaacgc ctcgcacgcc tcggccactc cgcggaagca gcggagcgtg atctcgggat
      301 ccggcgcgcc cccggaggcc agcacgaagc gcggcgtccc acccatcgcc gccacgtccg
      361 agacgttgac ggccacggcc ttgtatccca cgtcctccgg cgaggcccag cccttgaagt
      421 gatggccgga gaccagcatg tccgcggcgg ccacccagtc tttatctccc agccgcagca
      481 cggcgcagtc atccccgatg ggaaccagca cctcgggcgg cgtgccgggc aggaggtccg
      541 ttatctgctt tattacctcg aactcgttca acgccgtttc ttcccgaacc agttgcgagg
      601 attgaagaag cggcggtagc gggcggcgac ctccccgccg aagtacagcg ccgcacccca
      661 gaacacgacc tccgtagaga tcagtagccc cgagacggtc cagaccttga cctccgtcgg
      721 gagcggcaag aacgggacgg ccagcgtgga gagcgtcagg gccgccggta tcaccagcag
      781 gaacagcagt gggagccatc ttcggcggtg atcgtccgca cccttctctc cggctctggc
      841 tgcttccgga tctctccccg gctgtttatt cccgacttct atgcgcccat ctcccttacc
      901 ttactccaca ggacatcgga ttcgtcgttc gtgtaccagg tacgcagcga acgccattcg
      961 gtttccgtga cccagcggaa ggtgtcgtgg gcgacgtgct ccggtgagat cccacctccc
     1021 ggatgccgcg ccagaaagcc caccccggca tacaggtcgt ggtccctcgg accgcgccag
     1081 ggctcgaccc gcgcccacca cggagagacc ggctccgcct ccacccccgt ctcctcgaac
     1141 agctcgcgcc gggcgcaggc cacgaagtcc tcgccgccct ccagcatccc gccggggttc
     1201 tcccacagcc cgaccggcgg cttgcggcgg cgcaggagga gggcgcgcgg ctcggcgccg
     1261 tcgaagtcta cgaggaccgc cccggcaccc aggttcgccg ggacaacagc gtggtcccgt
     1321 ccgggctccg gcggctcggg ctccggcgac gggtctcgcg gaagctcgcg cagcggaccg
     1381 gcgacacccc ggatgttctc cttgctccac cagctcggca actccagcca ttcatcgagc
     1441 gtcacccatt tgtaaccgag gtgctcgtgc gagaggcgca cctcttcttc atccgtccgt
     1501 cctatatagg taacggccgc aagagacccg ccgttcgggg agtcgccgac ccaggtggcg
     1561 aggatgcgct ggggagcgac gagcattccc gtctcctcgt acagctcgcg cacggccccc
     1621 tcctggaacg tctcgccggt agcgagcctt cccgcaggcg gctcccaggt cccggcggga
     1681 cgctggagca ggagcatgcg gccgtcctcc cggacgggca ggatgccgga gacgaggtcc
     1741 gccctcaagg aaggtcccgc gcggttgtcg gcccagcact cacgcgcccg agtatagcgc
     1801 gagtcttacg cggcgctcat ttcgagccga ggaggagcga acgcagctcc ctggcggcgc
     1861 tctcagggtc ttcggcgtcg aggatcgccc ggacgacggc gaagccgtat gcgccggccg
     1921 ccgcgacctc cggggcggtc tccagcgtca cgccgccgat ggcgaaccag ggcacgggcg
     1981 ggtcctcgcg ggagatccgt tcgagcagcg ccactcccgc gacctccgta tcggtcttgg
     2041 tcggggtccc gtagatggtc cccaccccga ggtagtcagc gccctcccgc acggcctcgc
     2101 gagcttcttc gacgctcccc acagaccgtc cgacgatggt gtccgggccg agaagctccc
     2161 tcgcccgcgc cagtgggccg tcttcctgtc cgaggtggac cccatcggcg gccgagatcc
     2221 ttgcaagctc cacgtcgtcg tttaccgtaa agagggcgcc gcgagcacag acctccttca
     2281 actcctccgc gagcgggagc aggtcctccc ttgcagcacg cttgtcccgg agttgcacga
     2341 tatccactcc gccacggacg ccggccgcga cgcgctccgc caagtccggg cgcgggtccg
     2401 tgacgagcag gagccgcgtc ccgcgcaggc gctcgcgggg cttcacggcc cgaccctctc
     2461 gggcggccag gcgagcacgt cgagacggcg cgcgaagtgg aggaccggat cggcgcccgg
     2521 cagggagagg ccgatctgtc cggtcatctc cagcttctct acctcaacct ccgcgggttg
     2581 caggggccac atcacgtggt gcacgtcgcc gcgccggagc cgcccgtgcc ggtccgtcgc
     2641 gtagagggcg tatc
//
LOCUS       DASSMB010000277        10461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_488259, whole genome shotgun sequence.
ACCESSION   DASSMB010000277 DASSMB010000000
VERSION     DASSMB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488259"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VFJ72_15715"
     CDS             <1..509
                     /locus_tag="VFJ72_15715"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET7480952.1"
                     /translation="RPGDLLAVTGELGASAAGFLALEAGLDGPERLIRRHLRPKPRVE
                     AGLTAARLGAHAMIDLSDGLASDVRHICERSGVGCRLDLGSLPVAVDTRMLAESLGRD
                     AEVLAATGGEDYELLIAAPEGVIEALSDALDVPLAVIGEVTGSEVVFYRGGEVVEGLS
                     GWDHFRSG"
     gene            589..792
                     /locus_tag="VFJ72_15720"
     CDS             589..792
                     /locus_tag="VFJ72_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HET7480953.1"
                     /translation="MPYDRITELPTGVKNNLPKHAQEIYKEAFNSAEEQYGEESRAHR
                     VAWSAVEQKYEKNENGNWVSKDS"
     gene            complement(799..1488)
                     /gene="tenA"
                     /locus_tag="VFJ72_15725"
     CDS             complement(799..1488)
                     /gene="tenA"
                     /locus_tag="VFJ72_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET7480954.1"
                     /translation="MAGNKTIRERGFTADLWRSIDSIYAEIIAHPFLRGLTDGTLPME
                     CFRHYVVQDALYLRDYARALSLAGVRSPGEDAFLMFNEHAVGAITVERSLHAGFLKDL
                     DVSEADLDQTSPAPTTLAYTSYMLRRAALGDYHEVLGAVLPCYWIYAEVGRTLLENGS
                     PDPMYQTWIGTYGGEDFGAIVEAVLDLTDRACEDLSPAQRAAVTDAFVTTSRYEWMFW
                     NMGWTLEGWPV"
     gene            complement(1513..2544)
                     /locus_tag="VFJ72_15730"
     CDS             complement(1513..2544)
                     /locus_tag="VFJ72_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET7480955.1"
                     /translation="MRPFHGASPALRAMLALALISLAFTTAGCGKKESSDARITEFHP
                     ALGTYHAGEDATSLLRFENTSREPDTFWVGYSVRDGLGQWHDVPARRVKLESGKESAM
                     QYLDWTVPDDPPPPSGSYEVVMAVWSGKPGEDGAKRLADATKRDAFRVAGLDEEFAAL
                     NGERWDRPSKKLGRGSLAPDNVEVRSGRLEIKLPAGTRDGGEIQSAKLYQYGSYRARI
                     KVADAPSSITGFFLYSPPDFENEVDVEIYNDHNRRILFTTYSGGKQTNTVTKKLPFDP
                     TAGFHDYRFDLAPDRAEFYADGERLQTFRDGLPTGPMRLFVNSWFPAWLPGEKPEEDR
                     YTYVDRVRH"
     gene            complement(2541..4844)
                     /locus_tag="VFJ72_15735"
     CDS             complement(2541..4844)
                     /locus_tag="VFJ72_15735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480956.1"
                     /translation="MYQYYEERRRPGARSRILRATLIVFVLVLGVVAAFLFLNWGEVD
                     RPVSDSDGVKQVSTVKDAQLAVYDGSGWDKRFWTGMNLGATLPGHAPGELAPTKEDYL
                     RWFPEIKKMNVDVLRVYTILNPDFYEALREFNSTQKEPLWLIQGVWSPEEELTGADEE
                     GRDAYTPEITNIFRGEISDAVHVVHGDAQIPERPGHASGDYDADVSEYMLGWIVGTEW
                     FAPAVDLTDRSNPGTPPFSGRYFRATDDATPFESWGASMLDHLAEEEMKYGWQHPAAL
                     SNWPTTDPLSHPDEANRQEDLVSVDPMHIEPTSSWKAGYFASYHIYPAFPDFMRYEEK
                     YQNYRTADGTKDPYAGYLHELRAHHKGIPLVAAEYGTSSARGMAHRGALGRNQGMHTE
                     EAQGKINASLLDDIHNEGLDGGILFSWQDEWFKFAWNTGEMEIPSDRRPMWLNRLTNE
                     QNYGVIATEPGDSAEDMIHLDGKTDDWDRRTGGPDGAGQFFKWASDRILGRIPETRQS
                     RYGDFDLTVSQDEAYVYLLLKKHAGDWDFSKETADVGFGTLAGGSEKADKAPGLGFPD
                     GGAQFLLQIKGADDSHLWVNSAYDPHTWLYGDQLHYMPDPVIERDPQAGVFLPWRLAL
                     NRPLTLPQTKRKVPFEDYEVGKMKPGITDPSSKDYDSLADWYAKGGVLEVRIPWAMLG
                     FTDPSSLKVWDYPYEAGRIEAKKVDGLRVYPSIRATGETSDESVDPLDYAWDGWDQPS
                     YHERKKQSYYELKESFEGRDQVKPPPR"
     gene            complement(4884..6371)
                     /locus_tag="VFJ72_15740"
     CDS             complement(4884..6371)
                     /locus_tag="VFJ72_15740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7480957.1"
                     /translation="MTLNDFVLGVNYFVLGYFLLINGVYLVLYMISFTEIADYVRREV
                     FSGLPELFTSNYAPPVSVIVPAYNEEATIADSVRSFLTLHYPLHEVVVVNDGSKDGTL
                     DVLMEEFGLYESDQPVRMQLDTAPLRGVYASETERLVVVDKENGGKADALNVGICAAN
                     YPLVCCMDADIILEEDALLRIARPMIESSEVAAVGGIIRVANGCEFEKGRIIRARTPR
                     KALPNFQIVEYLRAFIASRTAWSKLNCLLIISGAFGMFRKRDLISAGGYQHDTVGEDM
                     ELTTRIHRALRENDRKYRLSFVPDPIAWTEVPDTLRVLRRQRDRWHRGLMDTLVRHRR
                     MLFNPRYGTVGLIGMPYFFLFEFLGPVIEILGYAVFIVGLLLGAINAPFALAFFLAAV
                     GLGVLLSTAAVFLEELRLERYPSWRDLFKLTVYGILENFGYRQINTLWRVLAVVSFLR
                     GNTNWGAMERKGFDPGKTGDKPEKRESPANLEDEPSEASERSKKT"
     gene            complement(6368..7603)
                     /locus_tag="VFJ72_15745"
     CDS             complement(6368..7603)
                     /locus_tag="VFJ72_15745"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HET7480958.1"
                     /translation="MGELTIIWIAVSAMILLNLLMVILTLGIRAARSVRRRSTEKRKR
                     RLEAALENSVFSDETDPALRNLDGRDLDLLALLMIDYLYVLSGEQRARLVALAEEAGL
                     LRRYLDRLEARGRWRKARAAENLGHFGGPENIEPLSKLLYHPDETLRAVAARALARIG
                     TPEAARELARRLGDPSELTRLRMAENLERIGAPSIEPLIEVLETARESGEVRLYGPVQ
                     AAQVLGQLRDSEARPALGHAALSGGSVDLRAKAALALGQVGDPEDLPTLFTAAEDEAW
                     PVRAQAASSLGAIGDLSAVPVLQKLTLDREWWVRLNASRSLANMGPAGERALASLLED
                     EDRYARDRAAATMEERGITRRAVEELTEPGERGESARAMIRAMVRAGATRYLGRLART
                     LPDEDARLALREIMAAEAS"
     gene            7692..8096
                     /locus_tag="VFJ72_15750"
     CDS             7692..8096
                     /locus_tag="VFJ72_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7480959.1"
                     /translation="MSEGGAKVLVADDDRVVRRIVTAKLSGLGYEVSEVRDGQEAIDR
                     LEDGERPDLLITDQLMPRMNGLQLVRAVRASSREGLADLPIIMLTSRGSERDIIEGLR
                     TGLDDYVVKPFSPDELAARVRTVLWRAQRLQS"
     gene            complement(8093..9061)
                     /locus_tag="VFJ72_15755"
     CDS             complement(8093..9061)
                     /locus_tag="VFJ72_15755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7480960.1"
                     /translation="MKILIAEDDAVSRMILQRAVEKFGHECLTAEDGEKALNLHRENP
                     DVDVIISDWMMPGMDGLELCRRVREERRDGYTFFIFLTALGDKEHLLEGMQAGADDYL
                     AKPLDREQLQVRLISASRVTSLHRQLNVKNKELEFLNEELFAMARRDPLTRLGNRLQL
                     REDLDTLSAQAERYGHSYCAVLCDVDHFKLYNDTYGHLAGDEVLKAVAGVMEKTVRKG
                     DTVYRYGGEEFLAILPEQPLESAAQAAERLRREVEDLKLPHEARDAPNVVTISLGLAI
                     LEPGEKKSAEKLLKQADDALYQAKKTGRNRMAVYEETAEEKEEAAG"
     gene            complement(9058..>10461)
                     /locus_tag="VFJ72_15760"
     CDS             complement(9058..>10461)
                     /locus_tag="VFJ72_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7480961.1"
                     /translation="GGTGLGLAISRQLVEIMGGRIWVESEPGAGSTFFFELPFEKQPR
                     QASPRPRTDLQGLRALVVDDNATNRQILDKQLSLWGMASDGADGSLEALRLLRAARER
                     DTRYDLAILDMQMPEMDGLSLARRIKEDPGLSSIRLVLLTSIGRRGEGGAARQAGIEA
                     YLTKPVRQQELYEALAAVMSETGHSGEAERRLVTRHSIRERQAGSRARVLLAEDNLVN
                     QKVAVRMLERLGYRVDVAGNGREALDAIRRNQYAAVLMDVQMPEMDGYEATAEIRWRE
                     RNEPGVRHLPVIAMTANAMQGDREKALAAGMDDYLPKPVKPDELRDVLDRWTGGQERD
                     SQDGEVFLGSHAAVDGAALDPSVLAGLQELQEDGEPDLIAELSVLFVQDSTLRLGTLR
                     EAVESEDARVIRETAHTLKGSSGNIGAWRMSRLAAELQDAGEARDLSRAEELVVRLEE
                     EFDRARLALDDLTGELK"
BASE COUNT         1575 a         3396 c         3568 g         1922 t
ORIGIN      
        1 cgcggcccgg cgacctcctg gccgtcaccg gcgagctggg agcgtcggcc gccgggttcc
       61 tggctctgga ggccgggctc gacggtcctg agcgcctcat ccgcaggcac ctgcgcccga
      121 agccgcgggt ggaggccggg ctgaccgcgg cccggctcgg ggcgcacgcg atgatagacc
      181 tctccgatgg gctggcctcc gacgtgcgtc acatctgcga gcggagcggg gtcgggtgca
      241 ggctggatct gggctctctg ccggtcgcgg tggatacgcg aatgctggcg gagtccctgg
      301 gacgggacgc ggaggtgttg gcggcgaccg gcggcgagga ctacgagctg ctcatcgccg
      361 cgccggaggg cgtgatcgag gcgttatcgg acgcgctgga cgtgccgctc gcggtgatcg
      421 gggaggtcac gggctcggag gtcgttttct atcgcggggg agaggtcgtg gagggtctct
      481 ccggctggga ccatttccgg tcgggctgag agaggctgag aggccggttc gctccgcggt
      541 aaaatcgggt agaaagaccg caggacaatt ttgcggaagg ggtcgggaat gccatacgac
      601 aggatcaccg aactgcccac gggcgtgaag aacaacctgc ccaagcacgc ccaggagatc
      661 tacaaggaag ccttcaactc cgccgaggag cagtacggcg aggagagccg cgcccaccgc
      721 gtagcctgga gcgccgtcga gcagaagtac gagaagaacg agaacggcaa ctgggtctcg
      781 aaggatagct gaccggctct ataccggcca gccttcgagc gtccagccca tgttccagaa
      841 catccactcg tagcggctgg tggtgacgaa ggcgtccgtg acggccgctc tctgagccgg
      901 gctcaggtct tcgcaggcgc ggtcggtgag gtcgaggacg gcctccacga tggcgccgaa
      961 gtcttctccg ccgtaggtgc cgatccaggt ctggtacata gggtcggggg agccgttctc
     1021 caggagcgtc ctgccgacct cggcgtagat ccagtagcac ggcagcaccg cgcccaggac
     1081 ctcgtggtaa tccccgagag cggcgcgcct tagcatgtag ctggtgtagg cgagcgtggt
     1141 cggggccgga gaggtctggt cgaggtctgc ctcggagaca tccaggtcct ttaggaagcc
     1201 ggcgtgcagg ctccgttcca cggtgatggc gccgacggcg tgctcgttga acatcaggaa
     1261 cgcgtcctct cccggagagc ggaccccggc gagcgagagg gcgcgggcgt agtcgcgcag
     1321 gtacagagcg tcctggacga cgtagtggcg gaagcattcc atggggagcg tgccgtctgt
     1381 gagaccgcgc aggaacgggt gagcaattat ctccgcgtag atggagtcta tagagcgcca
     1441 gaggtccgcg gtgaaccccc gctcacggat ggtcttgtta ccggccatga tcctcccttc
     1501 ggagcatttt ccctagtgcc gcacccgatc cacgtaagta tagcggtcct cctccggctt
     1561 ctctccgggg agccacgccg ggaaccagct gttgacgaac agcctcatcg gcccggttgg
     1621 caggccgtcg cggaaggtct gtagccgctc gccgtccgcg tagaactccg ccctatcggg
     1681 ggcaaggtcg aagcggtaat cgtggaagcc cgcggtcggg tcgaagggga gcttcttcgt
     1741 cacggtgttg gtctgtttgc cgccggagta ggtggtgaac aggatgcggc ggttgtggtc
     1801 gttgtagatc tccacgtcca cctcgttctc gaagtccggc gggctgtaga ggaagaagcc
     1861 ggtgatagat gacggagcgt ccgcgacctt tatcctcgcc cggtagctgc cgtactggta
     1921 gagcttcgcg gactgtatct cgccgccgtc gcgggtaccg gcgggcagct tgatctccag
     1981 acgcccggac ctcacctcga cgttgtccgg cgcgagagag ccccgtccca gcttcttcga
     2041 cggcctatcc caccgctcac cattcagggc ggcgaactct tcgtcgagtc cggccacccg
     2101 gaaggcgtct ctcttggtgg cgtcagccag ccgttttgct ccgtcctctc cgggcttccc
     2161 gctccacacc gccatgacca cttcgtagga gccggagggg ggcggcgggt cgtccgggac
     2221 ggtccagtcc aggtactgca tcgccgactc cttaccggat tcgagcttga cccggcgggc
     2281 gggaacgtcg tgccactgcc ccagcccgtc tcgcacgctg tacccgaccc agaaagtgtc
     2341 tggctcgcgg ctggtgttct cgaatcgcag gagggaggtc gcatcctcgc cagcgtggta
     2401 ggtcccgaga gcggggtgaa actccgttat cctggcatcg gatgattctt tctttccgca
     2461 tcccgccgtt gtgaatgcca gggagatcag ggccagggcc agcatcgcgc ggagagcggg
     2521 ggaagcgcca tgaaacggcc tcatcgcggc ggcggcttca cctgatcccg cccctcaaag
     2581 ctctctttta gctcgtagta gctctgcttc ttgcgctcgt gatagctcgg ctggtcccag
     2641 ccgtcccagg cgtagtctag cggatcgacg ctctcgtccg aagtctcgcc ggtggcgcgg
     2701 atggacgggt acacgcgcag gccgtccact ttctttgcct cgatcctgcc ggcctcatac
     2761 gggtagtccc acaccttgag gctgctcggg tcggtgaagc cgagcatcgc ccaggggatg
     2821 cggacctcca gcacgccgcc tttcgcgtac caatcggcga ggctatcgta gtccttgctc
     2881 gacgggtccg tgatgccggg cttcatcttt ccgacctcgt agtcctcgaa cgggaccttc
     2941 cgcttcgtct gcggcagcgt gaggggccgg ttgagggcga gcctccacgg gaggaagacg
     3001 ccggcctgcg ggtctctctc gatcacgggg tccggcatgt agtggagctg gtcgccgtag
     3061 agccaggtgt gcgggtcgta ggcgctgttg acccacaggt gcgagtcgtc cgcgcccttt
     3121 atctggagca ggaactgcgc gccgccgtcc gggaagccta ggcccggtgc cttatccgcc
     3181 ttctcgctgc cgcccgcgag cgtgccgaag ccgacgtccg ccgtctcctt cgagaagtcc
     3241 cagtctcccg cgtgcttctt caggagcagg tagacgtatg cctcgtcctg ggagacggtg
     3301 aggtcgaagt ccccgtaccg gctctgcctg gtctcgggga tgcggccgag gatgcggtcg
     3361 gacgcccact tgaagaactg cccggcgccg tccgggcctc cggtgcggcg gtcccagtcg
     3421 tcggtcttgc cgtcgaggtg gatcatatcc tcggcgctgt cgccgggctc ggtggcgatg
     3481 acgccgtagt tctgctcgtt tgtcagtcgg ttgagccaca tcggacgtcg gtcgctgggg
     3541 atctccatct ctccggtgtt ccaggcgaac ttgaaccact cgtcctgcca ggagaacagg
     3601 atgccgccgt cgagcccctc gttatggatg tcgtcgagga ggctggcgtt gatcttcccc
     3661 tgcgcctcct ccgtgtgcat cccctggttg cggcccagag cgccgcggtg ggccatgccg
     3721 cgggcggagg aggtcccgta ctcggcggcg acgaggggta tgcccttgtg gtgggcgcgc
     3781 agctcgtgca ggtatccggc gtaggggtcc ttcgtgccgt cggcggtgcg gtagttctgg
     3841 tacttctcct cgtagcgcat gaagtccggg aaggccggat agatgtggta ggaggcgaag
     3901 tagccggcct tccacgagga ggtgggttct atgtgcatgg ggtcgacgga gaccaggtct
     3961 tcctgacggt tcgcctcatc cgggtggctc agggggtcgg tggtcggcca gttggagagg
     4021 gcggcggggt gctgccagcc gtacttcatc tcctcctcgg cgaggtggtc gagcatcgag
     4081 gctccccagc tctcgaacgg cgtggcgtcg tcggtggcgc ggaagtacct gccggagaag
     4141 gggggcgtac cggggttgga gcggtcggtc aggtccacgg cgggggcgaa ccactcggtg
     4201 ccgacgatcc agccgagcat gtactctgag acgtccgcgt cgtagtcgcc gctggcgtgt
     4261 ccgggacgtt cgggtatctg cgcgtcgccg tggacgacgt ggacggcgtc cgagatctcg
     4321 ccccggaata tattcgtgat ctccggcgtg taggcgtcgc ggccctcctc gtccgcgccg
     4381 gtcagctctt cctccggcga ccagacgccc tggatgagcc acagcggctc tttctgggtg
     4441 gagttgaact cgcgcagagc ctcgtagaag tccgggttga ggatggtgta gacccgcagc
     4501 acgtccacgt tcatcttctt gatctccggg aaccaccgca ggtagtcttc cttcgtcggc
     4561 gccagctctc ccggcgcgtg ccccggcagc gtcgcgccca ggttcatccc ggtccagaac
     4621 ctcttgtccc aaccggaccc gtcgtagacg gcgagttgag cgtccttcac ggtcgagact
     4681 tgcttcacgc cgtcggagtc ggagacaggc cgatccacct ctccccaatt cagaaagagg
     4741 aacgccgcga ccacgccgag gacgagcaca aagacgatga gcgttgcgcg caggatgcga
     4801 ctcctcgcgc cgggccgtct tctctcttcg tagtactggt acacgctact ctccccgtca
     4861 ggtacttgcg atcttcgtaa gggttacgtt ttctttgatc tttcggatgc ttccgagggc
     4921 tcgtcttcga ggttcgcggg gctctctcgc ttctccggtt tgtctccggt cttgccgggg
     4981 tcgaagccct tgcgctccat cgcgccccag ttcgtgttgc cgcgcaggaa ggaaaccacg
     5041 gcgaggaccc gccagagggt gtttatctgc cggtagccga agttctctaa gataccgtag
     5101 acggtcagct tgaagaggtc gcgccagctc gggtagcgct ccaggcgcag ctcttccagg
     5161 aagaccgccg ccgtcgagag caggacgccg aggccgacgg cggcgaggaa gaaggccagg
     5221 gcgaacgggg cgtttatggc tcccagcagg agccccacta taaagacggc gtaacccagg
     5281 atctcgatga cgggacccag gaactcgaag aggaagaagt agggcatccc gatcaggccg
     5341 accgtgccgt agcgcgggtt gaagagcatc ctgcggtggc gcaccagcgt gtccatgagg
     5401 ccgcggtgcc agcggtcgcg ctggcggcgc agcacgcgca acgtatcggg gacctcggtc
     5461 caggctatgg ggtcggggac gaacgagagc cggtacttcc ggtcgttctc gcgcagggcg
     5521 cggtggatac gggtggttag ctccatgtcc tcgccgaccg tgtcgtgctg gtagccgccg
     5581 gcggagatca ggtcccgctt ccggaacatg ccgaacgccc cggagatgat gagcaggcag
     5641 ttgagcttgc tccacgccgt ccggctggcg atgaatgccc gcaggtactc gacgatctgg
     5701 aagttcggca gggccttgcg cggggtcctg gcccgtatga tgcggccctt ctcgaactcg
     5761 cagccgttgg cgacgcggat gatgccgccg accgccgcga cctcgctcga ctcgatcatg
     5821 ggacgcgcta tcctcaagag cgcgtcctcc tccagtatga tgtccgcgtc catgcaacaa
     5881 acgagcggat agttggccgc gcagatgccc acgttgagcg cgtcggcctt ccctccgttc
     5941 tccttgtcca ccaccaccag acgctccgtc tccgaggcgt agacgccgcg cagcggcgcg
     6001 gtgtcgagct gcatcctcac cggctggtcc gactcgtaga ggccgaactc ctccatcagc
     6061 acgtcgagag tgccgtcctt cgagccgtcg tttacgacga cgacctcgtg cagcgggtag
     6121 tggagggtga ggaaggagcg gacgctgtcc gctatggtcg cctcctcgtt gtaggccggg
     6181 acgatgaccg agacgggcgg ggcgtagttg gaggtaaaca gctccgggag gccggagaag
     6241 acctcgcgcc gcacgtagtc ggcgatctcg gtaaaggaga tcatgtacag gaccaggtac
     6301 acgccgttga tgagcaggaa gtagcccagc acgaagtagt tgacgccgag tacgaagtcg
     6361 ttgagcgtca cgaggcttcc gccgccatta tctcccgcag cgccagccgc gcgtcctcgt
     6421 ccggcagggt gcgggcgaga cgcccgaggt agcgggtcgc gccggcccgg accatcgccc
     6481 ggatcatggc ccgcgcgctc tcgccgcgct ctcccggctc ggtcaactcc tccacggccc
     6541 ggcgggtgat gccgcgctcc tccatcgtgg cggcggcccg gtcgcgggcg tagcggtcct
     6601 cgtcttcgag cagactcgcc agggcgcgct ccccggccgg ccccatgttc gccagcgacc
     6661 ggctcgcgtt caggcgcacc caccactccc ggtccagggt cagcttctgc aagaccggga
     6721 cggcggagag gtctccgatg gcgccgagcg agctcgccgc ctgcgcccgc acgggccagg
     6781 cctcgtcctc ggcggcggtg aagagcgtgg gcaggtcttc cgggtctccg acctggccca
     6841 gcgccagagc ggccttcgcc cggaggtcca cgctcccgcc ggagagcgcc gcgtggccca
     6901 gggccggacg cgcctccgag tcgcggagct gtccgagcac ctgcgccgcc tgcaccgggc
     6961 cgtagaggcg gacttccccg ctctcgcggg ccgtttcgag gacttcgatc aacggctcga
     7021 tggacggggc gccgatcctc tccaggttct cggccatcct gagccgggtg agctccgagg
     7081 ggtctccgag ccgcctcgcc agctcgcggg cggcctccgg cgttccgatg cgggccaggg
     7141 cgcgggcggc cacggcccgg agcgtctcgt ccgggtggta gagcagcttc gagaggggtt
     7201 ctatattctc gggaccgccg aagtgtccca ggttctcggc ggcgcgggcc ttgcgccagc
     7261 gcccgcgggc ctccagacgg tcgaggtatc tcctcagcag accggcctct tcggcgaggg
     7321 caacgaggcg ggcgcgctgc tcgccgctca ggacgtagag gtaatcgatc atcagcaggg
     7381 ccaaaaggtc caggtcccgg ccgtcgaggt tacggagggc cgggtcggtc tcgtcggaga
     7441 agacggagtt ctcaagggcg gcctccaggc ggcgcttccg cttctcagtg gagcggcggc
     7501 ggacggagcg ggcggccctg atgccgagag tcaggatcac catcagcagg ttcaggagga
     7561 tcatggccga gaccgcgatc cagattatcg ttagctcacc cattgatata gtactcgtcc
     7621 ataacctcta taatgtttag tggctggtcc gccggccgta agtgtacgcg catgatgggg
     7681 gttgtggatc tgtgagcgaa ggtggcgcga aggttctggt tgccgacgac gaccgggtgg
     7741 tgcggcgcat cgtgaccgcc aagctctccg ggctcgggta cgaggtctcg gaggttcggg
     7801 acgggcagga ggccatagac cggctcgaag acggtgagag gccggacctc ctgatcaccg
     7861 accagctcat gccccggatg aacggcctgc aactcgtgcg ggcggtgcgc gccagttcca
     7921 gagaaggact ggctgacctc ccgatcatca tgctcacctc caggggcagc gagcgggata
     7981 tcatcgaggg gctgaggacc ggcctggacg actacgtggt caagccgttc tcccccgacg
     8041 agctggccgc ccgcgtgcgg accgtcctct ggcgcgcgca acgtctccaa agctagcctg
     8101 ccgcttcttc tttttcttcg gcggtctcct cgtagacggc catccggttg cgccccgtct
     8161 tcttggcctg gtagagcgcg tcgtcggcct gcttgagcag cttctcggcg gacttcttct
     8221 cgcccggctc caggatcgcc agacccaggc ttatagtcac gacgttcggc gcgtcccttg
     8281 cctcgtgcgg cagcttcagg tcctcgactt cccggcgcag gcgttcggcg gcctgcgcgg
     8341 cgctctccag gggctgctcg ggcaggatcg ccaggaactc ctcgccgccg taacggtaga
     8401 cggtgtctcc cttgcggacg gtcttctcca tcacaccggc cacggccttc aggacctcgt
     8461 cgccggcgag gtgtccgtaa gtgtcgttgt agagcttgaa gtggtccacg tcgcagagga
     8521 ccgcgcagta gctgtgtccg taacgctcgg cctgggcgct cagggtatcc aggtcttccc
     8581 ggagctggag ccggttgccg aggcgagtca gggggtcgcg gcgcgccatg gcgaacaact
     8641 cttcgttgag gaattctagc tctttgttct ttacgttcaa ctgccggtgc agcgaggtta
     8701 cgcgggaggc cgagatgagc cgcacctgga gctgttcgcg gtcgagcggt ttggcgaggt
     8761 agtcgtcggc gccggcctgc ataccttcca ggagatgttc cttgtctccg agcgccgtaa
     8821 ggaagatgaa gaacgtgtac ccgtcgcgcc tctcctcgcg caccctgcgg cacagttcga
     8881 ggccatccat ccccggcatc atccagtcgc tgatgatcac gtcaacgtcc gggttctcgc
     8941 ggtgcagatt cagggccttc tcgccgtctt cggccgtcag acactcgtga ccgaacttct
     9001 ccacggcgcg ctgtaggatc atgcgcgaga cggcgtcgtc ttcggcgatc aggatcttca
     9061 ctttagctct cccgtcaggt cgtcgagcgc gagccgggcg cggtcgaact cttcttccag
     9121 tcggacgacg agctcctccg ctctcgaaag gtcccgcgct tctccggcgt cctgcaattc
     9181 ggccgcgaga cggctcatcc gccaggctcc gatgttgccg gagctgccct tgagggtgtg
     9241 agcggtctcc cgaatgaccc tggcgtcctc gctctccacg gcctctcgca acgtcccgag
     9301 acgaagggta gagtcctgga cgaagagtac cgaaagctcg gcgatgaggt ccggctcgcc
     9361 gtcctcctgg agttcttgca ggcccgccag caccgacggg tccagggccg ccccgtctac
     9421 cgccgcgtgg gaaccgagga agacctctcc atcctgggag tcccgctcct gtccgccggt
     9481 ccagcggtcg agcacgtccc gcagctcatc gggcttgacg ggtttgggga ggtagtcgtc
     9541 catgccggcg gcgagagcct tctcccggtc gccctgcatg gcgttggccg tcatggcgat
     9601 tacgggcagg tggcggactc cgggctcgtt gcgctctcgc caccggatct ccgcggttgc
     9661 ctcgtacccg tccatctccg gcatctgaac gtccataagc acagcagcat actggttgcg
     9721 ccggatcgcg tcgagagcct cgcgcccgtt gccggccacg tccacccggt atccgagccg
     9781 ctccagcatc ctgaccgcca ctttctggtt tacgaggttg tcctccgcga gcagcacgcg
     9841 ggcgcgggag ccggcctgcc gctccctgat gctgtggcgg gtgacgagcc gccgctccgc
     9901 ctctcccgaa tgcccggtct cgctcatcac cgcggccagg gcctcgtaga gttcctgctg
     9961 ccggacgggt ttggtcaggt aggcctcgat gcccgcctgc cgagccgcgc ctccttcccc
    10021 gcggcgcccg atggacgtca ggaggacgag ccggatggag gagaggccgg ggtcttcctt
    10081 gatgcggcgg gccagggaga gaccgtccat ctccggcatc tgcatgtcca ggatcgccag
    10141 gtcgtagcgg gtgtcccgtt cgcgggcggc gcgcaacaac cgcagggctt ccaggctccc
    10201 gtccgcgccg tcgcttgcca tcccccacag ggagagttgt ttgtcgagga tctggcggtt
    10261 cgtcgcgttg tcgtccacta ccagcgcccg cagtccctga aggtcggtgc ggggtctcgg
    10321 agacgcctgc cgcggctgct tctcgaacgg cagttcgaag aagaaggtgg agcctgcgcc
    10381 gggctcgctc tccacccata tgcgcccgcc cattatctcc accagctgcc tgctgatggc
    10441 gagccccagc cccgtccccc c
//
LOCUS       DASSMB010000278         5000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_488685, whole genome shotgun sequence.
ACCESSION   DASSMB010000278 DASSMB010000000
VERSION     DASSMB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488685"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VFJ72_15765"
     CDS             complement(<1..186)
                     /locus_tag="VFJ72_15765"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7480962.1"
                     /translation="MSPSRRCLAGSVGATRSLLAVPATNLKMVQKGLASSADGVFLDL
                     EDAVAPDRKEDARNDVIH"
     gene            complement(155..1687)
                     /locus_tag="VFJ72_15770"
     CDS             complement(155..1687)
                     /locus_tag="VFJ72_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET7480963.1"
                     /translation="MTDRHNGSSDHPRESGYSQQGGPGRVDSPEPAHEKAVAGTVGPG
                     RPSHGNLGQDAGAASASADKATVGRGRSESGQVIFPAFDAHHPPEQDLIDDCVHCGFC
                     LPTCPTYVLFGEEMDSPRGRIYLMNQGNQGEPMNDTMVRHFDLCLGCMACVTACPSGV
                     QYDKLIESTRAQVDRRYERTADDKAFREMIFQLFPYKNRLRLAASPLKLYQRFGIGDR
                     LRKAGVMKVLPARMRAMEALMPKLQKERKIPEYTAPVGEKRRRVGVLTGCVQQVFFSE
                     VNAATVRVLAAEGCEVLAPNQGCCGALSTHAGREEESLNFARKTIDTFGDLDLDNIVI
                     NAAGCGSTMKEYGYLLRDDPDYAEKAKAFSEKVRDVSEFLQELGPAAERNPLPVSAAY
                     HDACHLAHAQGIRKQPRDTLRQIPGMEIKEIREAEICCGSAGIYNMVEPEAAGQLGER
                     KANNILKTGAQMIVTSNPGCMLQIQASLKKMGHDGLPMAHPMEVLDASLRGEPVETLL
                     GR"
     gene            complement(1687..2973)
                     /locus_tag="VFJ72_15775"
     CDS             complement(1687..2973)
                     /locus_tag="VFJ72_15775"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7480964.1"
                     /translation="MSVSEVAQVSVEDLQSIVGREHARDASEADAVDGVAPSFVVEPG
                     TVEEASAVMKLAHEKGLKVTPRGSGTKIGWGNPPSEVDLIVSTARLNRLLEHAAGDLV
                     TRVEAGMKLEDLQKELAGANQMLALDPPEAGATVGGIVAANASGPRRLRYGTVRDLLI
                     GITVVLPNGTVAKAGGKVVKNVAGYDLGKLYTGSLGTLGLIVETIWRLHPIPETSRTV
                     VVEVESPEEAGAAVQSLMHSTLVPTMIEVHRPESNGATVAALIEGIEPGVLAQSVSAL
                     ELTRLHGDARVLERDEVAGFWRDALKTPWDESGVGLKIGALPAELPNILQLVSDAAER
                     NGLTPRVTGHAATGVTLVGLSGGDDEGYAAMVQELREIIGSGEGSVVVVQGSPELKRK
                     VDAWGAVGDSLPLMRRVKGRFDPGALMNPGRFVGGI"
     gene            complement(2973..4427)
                     /locus_tag="VFJ72_15780"
     CDS             complement(2973..4427)
                     /locus_tag="VFJ72_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7480965.1"
                     /translation="METATLVERMRGILGAEGVISEHEQLRTYECDGLMSYRVVPRIV
                     VLPETADQLQRIVQVCYEEGIPFVARGSGTGLSGGALPHEEGVLIALARMREIVEVDI
                     PNQRVIVEPGVINLWVTQEVAPEGYYFAPDPSSQLVCSIGGTVAENSGGAHCLKYGFT
                     TNHVTALEVVLPDGEVVHLGSKAPDPPGYDLLGAFVGSEGTLGIATKVTLRIVRQPET
                     VKTLLAAFPTVDEAGAAVSEIIASGIIPSAAEIMDSLSIQAAEMAVHAGYPEGTGGIL
                     VAELDGPQAEVDHQFGEVERICRENHATEVRVARDEEERVVLWKGRKAALAAMGRISH
                     DCYLQDSVVPRTALSDVLKKIAALSDEWGLRVANFFHAGDGNLHPLVLYDERIEGESE
                     KAHELAGQIVLACLEYGGSITGEHGVGEDKKHYMPKMFTENDMNTMQLLRCAFDPHGL
                     ANPGKVFPTPRLCGEVPGPYKVHPLQKAGMAEMF"
     gene            complement(4427..>5000)
                     /locus_tag="VFJ72_15785"
     CDS             complement(4427..>5000)
                     /locus_tag="VFJ72_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7480966.1"
                     /translation="RICEENNSTEIRIAEDDAQRALFWKGRKAAFAAVGRISPDYFVQ
                     DGVIPRTALPEVLRKIKEMSDQHGLRVANVFHAGDGNLHPLVLYNGNVEGEAERAEEL
                     AGEILVLCVELGGSITGEHGVGEEKKHYMPKMFSEDDLNTMQLLRCAFDPHALSNPEK
                     VFPTPRLCGEVRPGPRRIHPVEKAGLAQVF"
BASE COUNT          733 a         1755 c         1604 g          908 t
ORIGIN      
        1 gtggatgacg tcgtttcggg catcctcttt cctgtccggg gcgacggcgt cttcgaggtc
       61 gaggaagacg ccgtcggcgc ttgaggccag tcccttctgg accatcttga ggtttgtggc
      121 cgggacggcc aggagggagc gggtcgcgcc cacgctacct gccaagcagc gtctcgacgg
      181 gctcaccgcg cagggaggcg tcgaggacct ccatcgggtg agccatcggg aggccgtcgt
      241 ggcccatctt cttgagggac gcctgtatct ggagcatgca gccggggttg gaggtgacga
      301 tcatctgcgc gccggtcttg aggatgttgt tcgccttgcg ctcgccgagc tgtccggcgg
      361 cctcgggctc gaccatgttg tagatgccgg cggagccgca gcagatctcc gcctccctta
      421 tctccttgat ctccatgccc ggtatctggc gcagcgtgtc gcgcggctgc ttcctgatcc
      481 cctgcgcgtg cgcgaggtgg caggcgtcgt ggtaggccgc cgagaccggg agcgggttcc
      541 tctccgcggc cgggccaagc tcctgcaaga actccgatac gtcccggact ttctcggaga
      601 acgctttggc cttctccgcg tagtcggggt cgtcgcgcag caggtagcca tactccttca
      661 tcgtcgagcc gcacccggcg gcgttgatga cgatgttgtc caggtcgagg tctccgaagg
      721 tgtctatggt cttgcgggcg aagttcaggg actcctcctc gcgcccggcg tgggtggaga
      781 gggcgccgca gcagccctgg ttgggagcga ggacctcgca tccctcggcg gccaggacgc
      841 gcacggtggc ggcgttgacc tcggagaaga aaacctgctg cacgcaaccg gtcaggacgc
      901 ccacgcggcg gcgcttctcc ccgacggggg cggtgtactc ggggatcttg cgctccttct
      961 ggagcttggg catgagcgcc tccatcgccc tcatgcgggc ggggaggacc ttcatcaccc
     1021 cggccttcct gagccggtcg ccgatgccga agcgctggta gagcttcagg gggctcgcgg
     1081 cgaggcgcag acggttcttg taggggaaga gctggaagat catctcccgg aaagccttgt
     1141 cgtccgccgt ccgttcgtag cggcggtcta cctgcgcgcg ggtggactcg atgagcttgt
     1201 cgtactgcac gccggacggg catgccgtga cgcaggccat gcagccgagg cagaggtcga
     1261 agtggcgcac catcgtgtcg ttcatcggct cgccctggtt gccctgattc atcaggtaga
     1321 tgcgcccgcg cggggagtcc atctcctcgc cgaagaggac gtaggtcggg cacgtcggca
     1381 ggcagaagcc gcagtggacg cagtcgtcta tcagatcctg ctccggcgga tgatgcgcgt
     1441 cgaaggctgg gaagatgacc tgcccactct ccgagcgtcc cctgccgacc gtcgccttgt
     1501 cggccgacgc gctcgccgcc ccggcgtcct ggccgaggtt gccgtgcgag ggacggcccg
     1561 gccctacggt cccggctacg gccttctcgt gggcgggctc cggggagtcc acccgtccgg
     1621 gaccgccctg ctgagagtaa ccggactccc tggggtgatc cgaggagccg ttgtgcctgt
     1681 ccgtcattag atacctccca cgaacctgcc ggggttcatc aaggcccccg gatcgaaacg
     1741 tccctttacg cgccgcatga gcggcagcga gtctccgacc gccccccacg catccacctt
     1801 ccgcttcaac tcgggcgagc cctggaccac caccacgctc ccctcgccgc tgccgatgat
     1861 ctcccgcagt tcctgcacca tagcagcgta gccctcatcg tctccgccgg agagaccgac
     1921 cagcgtaacc ccggtcgccg catgtccggt tacccgaggc gtgagaccgt tccgttccgc
     1981 cgcgtccgag accagttgca ggatgttcgg caactccgcc gggagcgcgc cgatcttcag
     2041 accgacgccg ctctcgtccc aaggcgtctt cagggcatct ctccagaaac cggctacctc
     2101 gtcccgttct agaaccctgg catcgccgtg gaggcgcgtg agctccagcg ccgacaccga
     2161 ctgcgccagg acgccgggct ctatgccctc gatcagggcg gcgaccgtgg cgccgtttga
     2221 ctcgggcctg tggacctcga tcatggtcgg caccaaggtg gagtgcatga ggctctgcac
     2281 cgccgcgcca gcctcctccg ggctctcgac ctccacaacg accgtccggc tcgtctccgg
     2341 gatcgggtgc aggcgccaga tcgtctccac gatcaacccc agcgtcccca acgaacccgt
     2401 gtagagcttg ccgaggtcgt agccggccac gttcttgacg acctttcctc ccgccttcgc
     2461 cacggtcccg ttcggcaaca cgacggtgat cccgatgagc aggtcccgca cggtcccgta
     2521 gcgcagccgc ctcggccccg aggcgttggc cgcgacgatc ccaccgaccg tcgcgccggc
     2581 ctccggcggg tcgagggcga gcatctggtt cgcgccggcg agttccttct gcaggtcctc
     2641 cagcttcatc ccggcttcga ccctggtcac gaggtcgccg gcggcgtgct ccaggagccg
     2701 gttgagccgc gcggtgctga cgatgaggtc cacctcggag ggcgggttgc cccacccgat
     2761 cttggtcccc gacccgcgag gcgttacctt cagccccttc tcgtgcgcga gcttcatcac
     2821 cgcgctcgcc tcttccaccg tccccggctc gacgacgaag gacggcgcaa cgccgtccac
     2881 ggcgtccgcc tcgcttgcgt cgcgggcgtg ctccctgccg acgatgctct ggagatcctc
     2941 caccgacacc tgcgccacct ccgatacgct catcagaaca tctccgccat tccagccttc
     3001 tgcaacgggt gaaccttata cggacccggc acctccccgc acaacctggg cgtggggaag
     3061 accttgccgg ggttggcgag gccgtgcggg tcgaaggcgc accgcaagag ttgcatggtg
     3121 ttcatgtcat tttcggtgaa catcttcggc atgtagtgtt tcttgtcctc tcccacaccg
     3181 tgttcgccgg tgatggagcc gccgtactcc aggcaggcca ggactatctg cccggccaac
     3241 tcgtgcgcct tctcggactc gccctcgatc cgctcgtcgt acaacaccag cgggtgcagg
     3301 ttgccgtcgc ccgcgtggaa gaagttcgcc acgcgcagcc cccactcgtc gctcagggcg
     3361 gctatcttct tcaatacgtc cgagagggcc gtgcggggta cgacgctgtc ctgcaggtag
     3421 cagtcgtggg agatgcggcc catcgccgcc agcgccgcct tgcggccctt ccacagcacc
     3481 acgcgctcct cctcgtcgcg ggcgacccgg acctccgtgg cgtggttctc ccggcagatc
     3541 ctctcgacct ccccgaactg gtggtccacc tccgcctgcg gcccgtcaag ctcggcgacc
     3601 aggatgccgc cggtcccctc agggtagccc gcgtggaccg ccatctccgc cgcctggatg
     3661 gagagggagt ccatgatctc cgccgccgaa ggaatgatcc ccgacgcgat gatctccgac
     3721 accgccgccc cggcctcgtc aaccgtcggg aacgccgcaa gcaaggtctt caccgtctcc
     3781 ggctggcgca cgatcctgag cgtcaccttc gtggctatcc ccagagtgcc ctccgaacca
     3841 acgaaagcgc cgagcagatc gtagcctggc gggtccggcg ccttgctgcc aagatgcacg
     3901 acttctccgt ccggcagcac cacctccaaa gcagtcacat ggttcgtcgt gaagccgtac
     3961 ttcaggcaat gggcgccgcc ggagttctcg gcgacggtcc cgccaatgga gcagaccagt
     4021 tgactcgatg gatctggggc gaagtaatag ccctccggcg ccacttcttg tgttacccag
     4081 aggtttatga cgccgggctc gacgatcacg cgctggttcg ggatgtccac ctcgacgatc
     4141 tcgcgcatgc gggcgagggc gatcaatacc ccttcctcgt gcggcagggc gccgccggag
     4201 aggccggtgc ccgagccgcg cgccacgaac gggatgcctt cctcgtagca gacttgcacg
     4261 atccgctgga gctggtcggc ggtctcgggg aggacgacga tgcgcgggac gacccggtag
     4321 ctcatcaggc cgtcgcactc gtaggtacgc agttgctcgt gctcggagat aacaccttcc
     4381 gcgcccagga tcccccgcat cctctctacg agcgtagcgg tctccactag aacacctgcg
     4441 ccagtccggc tttctcgacc gggtgtatgc gccgcggtcc gggccggacc tctccgcaca
     4501 ggcgcggcgt cgggaagact ttctcggggt tggagagcgc gtgcgggtcg aaggcgcagc
     4561 gcaggagctg catggtgttc aggtcgtcct cgctgaacat cttgggcatg tagtgcttct
     4621 tctcctcgcc gacgccatgc tcgccggtga tggagccgcc gagctcgacg cagagtacca
     4681 gtatctctcc ggcaagctcc tcggcgcgct cggcctcacc ctcgacgttg ccgttgtaga
     4741 ggacgagggg gtgcaggttt ccgtcgcccg cgtggaagac gttcgccacg cgcaggccgt
     4801 gctggtcgct catctccttg atcttgcgca gtacctcggg gagggcggtg cgtgggatca
     4861 cgccgtcctg cacgaagtag tcggggctga tgcgaccgac cgccgcgaac gccgccttgc
     4921 gacccttcca gaacagcgcc cgctgggcgt cgtcctcggc gatccggatc tcggtcgagt
     4981 tgttctcctc gcagatcctg
//
LOCUS       DASSMB010000279         7124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_722590, whole genome shotgun sequence.
ACCESSION   DASSMB010000279 DASSMB010000000
VERSION     DASSMB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7124
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722590"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFJ72_15790"
     CDS             <1..203
                     /locus_tag="VFJ72_15790"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HET7480967.1"
                     /translation="DGYVHAGVQATMVDRTAGAAAGALIARGQLRLTVEFKINLLRPG
                     RAAPLRSAGPDLRGRRDPGRPG"
     gene            complement(258..947)
                     /locus_tag="VFJ72_15795"
     CDS             complement(258..947)
                     /locus_tag="VFJ72_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HET7480968.1"
                     /translation="MREPAKRDDRTAPLALVIHTSDEPGTLHELTRVIFEHRANITYV
                     DIAERREDSSTVYFELEDVAGGAADVLIGDLSALPIVRGVEQAPSFAKIYGKRIIVVG
                     GGAQVGQVVVGAVAEADRHNIRGERISIDTIPLVGEENLAAAARAVARLPRAVALVLA
                     GALMGGDVAQAVREIREKGIIVLSLNMPGSVPDAADLVVTDPVQCGVMAVMAVSSSGR
                     FDIRRQRGRRY"
     gene            complement(1114..2046)
                     /locus_tag="VFJ72_15800"
     CDS             complement(1114..2046)
                     /locus_tag="VFJ72_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7480969.1"
                     /translation="MRQDVTFTSEGLNCAGWLYVPDDLEGGERRPAVVMAHGFSAVKE
                     MYLANFAEKFEEAGLVVLVFDYRYFGDSEGEPRGRLFPAEQHEDYKNAISWVSQRPEV
                     NPDRIGVWGSSYSGAHVLHLAAFDRRIKAAVSQVPLVDGWANAQRLMRADIFAGFHEM
                     LAEDRLARYGGEESARIPVVAPEGEPSALPTPDSYEWFTQTGQSIAPNWLNEVSLESM
                     EKFLEYNPAANIHRISPTPLLMIVAGRDTLTPTDLAVAAYERALEPKSLVITGGAHFD
                     AYTEPGLSETAIPAVQWFERYLLGRQFVEVETAR"
     gene            2297..3178
                     /locus_tag="VFJ72_15805"
     CDS             2297..3178
                     /locus_tag="VFJ72_15805"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HET7480970.1"
                     /translation="MPESVGFVGLGIMGRPMARNLMAAGYDLVVHNRSPARADELAAE
                     GAAAARSPREVAEKSGVVITMLPGPPEVREVVTGEDGLLEGASAGSLIIDMSTSSPVL
                     ARKLALEAGKRKIGMLDAPVSGGDVGAIEGTLSIMVGGDENDFERAKPLFEVMGKTVT
                     HVGPAGAGQVTKAANQIVVALVIEAVSEALVLGSKGGVAPEKILDVLGGGLAGNKVME
                     VKREKFLSRDFGPGGKVEYHLKDLRIALDTAREYEVALPVTAIVDQLFAALVAKGRGG
                     WDHSALLTLIEDMSGSG"
     gene            3214..3918
                     /locus_tag="VFJ72_15810"
     CDS             3214..3918
                     /locus_tag="VFJ72_15810"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7480971.1"
                     /translation="MSEANKALVRRWLDEVFSRGDLGGVGEIFAPEYVLHDPSFPLDV
                     HGLEGVKKYVATYRAAFPDARFAVEDQIAEGDEVVTRWTAQGTHEGEFLGLAPTGEKV
                     TVSGIEFDHVAGGRISEAWVGYHPFAGPSPDPEQLRGGVAAMREAFSDLRVIEAASIR
                     EGDMAAFRWLMAGTQQGEFLGVSPTGKRVEAMGMDIVRLADGEIAEHWGEFDAIGLLR
                     QLGVIPPPGQTGDERA"
     gene            3977..4654
                     /locus_tag="VFJ72_15815"
     CDS             3977..4654
                     /locus_tag="VFJ72_15815"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7480972.1"
                     /translation="MFDVRASDRLAAWRDRRTVPEGRFEEVPHGDPYGLLGVPRGASQ
                     EDVRRAYRKLVRQHHPDANPEDPAAEERFKEIQAAYETLSDPKKRREHDERSRSSTRK
                     RSTSSRKASSGGTRRQNVGSVDLSDLLSNLGDVSSRTGGRKEVNWQLRGEDLARVAKV
                     LGLDITRISKLLGENIKANTKVSFGNRPTDGSRKTSESAASGMPPKPRKPPKPNKPPK
                     TRKPRDV"
     gene            complement(4685..5185)
                     /locus_tag="VFJ72_15820"
     CDS             complement(4685..5185)
                     /locus_tag="VFJ72_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7480973.1"
                     /translation="MSYEPIPAETRIGHVHLRVSDLDRAIEFYRDLMGFDLVTRLGDE
                     AAFLSAGGYHHHIGLNTWQSKGGSPPPHGTTGLYHFAINYPARRDLAVALKRLLDNDW
                     SIDGASDHGTHEAIYLHDPDHNGIELAWDRDQSQWPRIGDQVNFTRRPLDFNSLLAEI
                     EEPVSR"
     gene            complement(5227..5856)
                     /locus_tag="VFJ72_15825"
     CDS             complement(5227..5856)
                     /locus_tag="VFJ72_15825"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7480974.1"
                     /translation="MKLLIIGAAGILGSRILQEAVSRGHDVTAVVRREGALSSEQDVR
                     VVQADVRDAGVAASIFAGHDAVVVAVSARRGGDAPVPEVVRAAIDGARDASVTRLFVL
                     GGAGSLEVAPGVQLLDAPGFPEAYRAEAIQGREALNVIRDSDVNWTYLSPAAEISPGE
                     RTGRYRVGGDQLLTDAEGKSFISAEDYAVAVLDELEHPRHERQRFTVAY"
     gene            complement(5928..6566)
                     /locus_tag="VFJ72_15830"
     CDS             complement(5928..6566)
                     /locus_tag="VFJ72_15830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HET7480975.1"
                     /translation="MNVTIIGSGNMARGIGDRLVAGGNSVRIIDRDPEKAGALAGELR
                     GVDGAKAEAGSLGDPIEGDIVILAVPYGAVESIAGQYGSGLAGKVVVDITNPVDWESF
                     DRLVTPPDSSAAEEIGNLLPESAVVKAFNTTFAGTLVGGEVDGQPLDVFVAADDTDAK
                     QTVISLIEAGGLRPIDAGPLRRARQLEALGFLHMTLQQPLGTGFGSTVKVLG"
     gene            complement(6593..7003)
                     /locus_tag="VFJ72_15835"
     CDS             complement(6593..7003)
                     /locus_tag="VFJ72_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7480976.1"
                     /translation="MLDVGLLVLRVVLGVIFAAHGAQKLFGSFGGPGLKGTAGFHEQL
                     GIKPPYLMAVLAGLAEFAGGILVAVGLLTPLAAVALIVTMAVAALTVHLKNGFFAMNG
                     GYEFNLALAGMALTLLLTGAGAISLDAALGIVLW"
BASE COUNT         1277 a         2310 c         2343 g         1194 t
ORIGIN      
        1 aagacgggta cgttcacgcc ggggtgcagg cgacgatggt ggaccgtacc gccggcgccg
       61 cggcgggcgc cctgatagcg cgaggccagc tacgtttgac tgtcgagttc aagatcaacc
      121 tgctccgacc ggggcgagcg gctcctctgc gaagcgcggg tcctgacctc cgaggccgcc
      181 gtgaccctgg ccgccctgga taggcgaaca tccgaggggc agcccgcgta gccgataccg
      241 gcacggccgc cgggctgtca gtagcgccgg cctcgctgtc ggcggatgtc gaagcgcccg
      301 gaggacgaga cggccatcac ggccatcacg ccgcactgga caggatcggt caccaccagg
      361 tcagcggcgt cggggacgct gccgggcatg ttgagggaga ggacgatgat ccctttctcc
      421 cggatctcgc ggactgcctg ggcgacgtcg ccgcccatga gcgctcccgc caggacgagc
      481 gccaccgctc gcggaaggcg agcgacggcc cgcgcagcgg cggccaggtt ctcctcgccc
      541 accagcggga tggtgtctat tgagatacgc tctccgcgga tgttgtggcg gtcagcctcg
      601 gcgacggctc ccaccaccac ctgtcccacc tgggctccgc ctccgacgac gatgatccgc
      661 ttgccgtata tcttggcgaa cgagggagcc tgctcgaccc cccgcacgat aggcaacgcg
      721 ctgaggtcgc cgatcaggac gtcggcggcc ccgccggcga catcttcgag ctcgaagtag
      781 accgtggaac tgtcctcgcg ccgctcggcg atgtccacgt aggtgatgtt cgcccggtgc
      841 tcgaagatca cacgggtcaa ctcatggagc gtgccgggct cgtccgatgt atggattacc
      901 agggccagcg gggcagtccg gtcgtcacgt ttcgcgggct ccctcacgga cgcgcgactc
      961 cggcggtccg gcacacagag aatgtcgtat ctacgcgggg cacggctaga gtttaagaca
     1021 gagcgtccct cgacgctcgg acgccgcgat cttctccagg ttcaagcccg ccggagttgc
     1081 gtgactgacg cctccgggag tgccgcgccg atctcagcgc gcggtctcga cctcgacgaa
     1141 ctggcgtccg agaagatacc gctcgaacca ctgcacggcg gggatcgcgg tctcggagag
     1201 ccccggctcg gtgtaggcgt cgaagtgcgc gccgcccgtg atcaccagcg acttcggctc
     1261 cagggcccgt tcgtaggccg ccaccgccag gtccgtcggg gtcagggtgt ctctgccggc
     1321 gacgatcatc aggagcgggg tgggtgagat cctgtgtatg ttcgccgccg ggttgtactc
     1381 caggaacttc tccatggact ccaggctcac ctcgttgagc cagttcgggg cgatgctctg
     1441 tccggtctgc gtgaaccact cgtaggagtc cggcgtcggc agcgcggacg gctcgccctc
     1501 gggcgcgact accggtatgc gggcgctctc ctctcccccg taccgcgcga gacggtcctc
     1561 ggcgagcatc tcgtggaacc cggcgaagat gtccgcgcgc atcagccgct gggcgttggc
     1621 ccacccatcc actagcggca cctgggacac cgcggccttt atgcgccggt cgaacgccgc
     1681 gaggtgcaag acgtgcgcgc cgctgtagga cgagccccac acaccgatgc ggtccgggtt
     1741 gacctcgggc cgctgagaga cccagcttat ggcgttcttg tagtcctcgt gctgctccgc
     1801 cgggaacagc ctaccgcgcg gttcgccctc cgagtctccg aagtagcggt agtcgaagac
     1861 cagcaccacg agccccgcct cctcgaactt ctcggcgaag ttggcgaggt acatctcctt
     1921 gacggcggag aacccgtgcg ccataaccac cgcgggacgc cgctctccac cctccaggtc
     1981 atccggcaca taaagccacc ccgcgcagtt cagcccctca ctcgtaaacg ttacgtcctg
     2041 tctcatgtcg ctcccttcct aaccgctcac cagcgcagat cgcccccgga atagggtcgg
     2101 cgcatcccgc gaacgtaccg tgacaaactc taccgccgct cctttccgca tatccccgac
     2161 cgccatgtta tatgccttac tatcccgcca caagaggtct catggcgcgg gtttccgggc
     2221 tggctagtct gcgaagatac gctggggtaa gctggtagtt gccgtcgcag gagtcacgga
     2281 cgcataggga ggcgggatgc cggagagcgt gggtttcgtg gggttgggga tcatgggcag
     2341 accaatggct cgtaacctca tggcggcggg ctatgatctg gtggtccaca accgcagtcc
     2401 tgccagagcc gatgagctgg ccgccgaggg agccgcggcc gctcgcagcc cgagagaggt
     2461 cgccgaaaag agcggcgtcg tgatcaccat gctcccgggt ccgccagagg tgcgcgaggt
     2521 cgtgaccggc gaagacggcc tcctggaagg agccagcgcg ggctccctca tcatagacat
     2581 gtccacctcc tctccggtcc tcgccagaaa actcgccctg gaagccggta aacgtaaaat
     2641 cgggatgctg gatgccccgg tctcgggcgg agatgtcggg gccatcgagg gtacgctctc
     2701 catcatggtt ggtggcgacg agaatgactt cgagcgggcg aagcccttgt tcgaggtgat
     2761 gggcaagacc gtcacgcacg tcgggcctgc gggggcgggg caggtcacga aggcggccaa
     2821 ccagatcgtg gtggcgctcg tcatcgaggc ggtctctgag gcgctggtgc tcggctcgaa
     2881 gggcggggtt gcgccggaga agatcctgga tgtcctggga ggagggcttg cgggcaacaa
     2941 ggttatggag gtgaagcggg agaagtttct ctcgcgcgac ttcgggccgg gtggcaaggt
     3001 cgagtaccac ctcaaggacc tccgcatagc gctggatacc gcccgagaat acgaggtagc
     3061 cctcccggtc acggccatag tagaccagct cttcgcggct ctggtggcga aggggagagg
     3121 tggttgggac cactcggccc tgctcaccct catcgaagat atgtccggga gcggatagcg
     3181 cagccccgtt atcgagccgc aaggagactc ccgatgagcg aagctaacaa ggctctggtc
     3241 cgcaggtggc tcgacgaggt gttcagccgg ggagacctcg ggggagtagg cgagatcttc
     3301 gctccagagt acgtcctcca cgatcccagt ttcccccttg acgtgcatgg ccttgagggc
     3361 gtaaagaagt atgtagcgac gtaccgcgcg gccttccccg atgcccgctt cgcggtcgag
     3421 gaccagatcg ccgaaggaga tgaggtggtc acccggtgga cagcgcaggg aacccatgaa
     3481 ggtgagttcc tggggctcgc tcccaccggg gaaaaggtaa cggtgagcgg gatcgagttc
     3541 gatcacgtag ctggaggcag gatctccgag gcgtgggtcg gctaccaccc gttcgcggga
     3601 ccatcgcccg accccgaaca gctccgtgga ggggtcgccg ccatgcgcga agcattctcc
     3661 gacctgcggg tgatcgaagc cgccagcatt cgggaaggcg acatggcggc cttccgctgg
     3721 ttgatggccg gcacccaaca gggcgaattt ctcggcgtct cccccaccgg caaacgggtc
     3781 gaagccatgg gcatggacat cgtgaggctc gcggacggag agatcgccga gcactggggc
     3841 gagttcgacg cgatcggtct gctgcggcag ttgggagtta tacctccgcc gggacagacc
     3901 ggggacgaac gcgcatagga ccgacctcgc tccccggccg ggtttgtgat agccgggaga
     3961 ttgggaaagc ataggcatgt tcgatgttcg cgcgtccgac cggctcgcgg cgtggcggga
     4021 ccggcgcacg gtaccggaag ggaggttcga ggaggtgccc cacggagatc cctacggcct
     4081 gctcggcgta cccagaggag cctcgcagga ggacgtgcgc cgggcgtacc ggaagctggt
     4141 ccgccagcac cacccggacg caaacccgga agatcccgca gccgaggagc ggttcaagga
     4201 gattcaggcc gcttacgaga ccctctcgga cccgaagaag cgccgggagc acgacgaaag
     4261 gtctcgatcc tctacccgga agagatctac ttcgtcccgc aaggccagca gcggcggaac
     4321 ccggcgtcag aatgtaggct ccgtagacct ctccgacttg ttgagcaatc tcggcgacgt
     4381 ctcgagccgg accggcggac gcaaggaggt aaactggcaa cttcggggag aagacctggc
     4441 ccgcgtggcg aaggtactgg gcttggatat cacccgaatc tcgaaactcc tcggcgagaa
     4501 tatcaaggcg aacacgaaag taagttttgg aaacagacct accgatggtt cccgcaagac
     4561 gtcggaaagc gcggcttccg ggatgcctcc gaagccgcgc aaaccgccta aacctaacaa
     4621 accgccgaag acccgcaaac ctcgcgacgt ctgagcgtgt agaggaaacc ctgtagagcc
     4681 gactctagcg ggagaccggc tcctcgatct ccgccaggag gctattgaag tccagcgggc
     4741 gcctcgtaaa gttgacctgg tcgcctatcc gcggccactg actctggtcc cggtcccagg
     4801 ccagctcgat gccgttgtgg tccgggtcgt ggaggtatat agcctcgtgg gtgccgtggt
     4861 cagaggcgcc gtcgattgac cagtcattgt ccagcaggcg cttgagcgcg acagccaggt
     4921 ccctgcgagc cgggtagttg atggcgaagt gatagaggcc ggtcgtgcca tgtgggggcg
     4981 gagagcctcc cttgctttgc caggtgttca ggccgatgtg gtgatggtag ccgcccgccg
     5041 agaggaaagc cgcctcgtca ccgaggcgcg tgacgaggtc gaagcccatt aggtcccggt
     5101 agaactcgat ggctcggtcg aggtccgaga ccctgagatg cacgtggccg atcctggtct
     5161 cggcggggat gggttcgtaa ctcatgatca atatcctttc gtagtgccta tcgatctctc
     5221 agtatctcag tacgcgacgg tgaagcgctg gcgctcgtgc ctcgggtgtt ccaactcgtc
     5281 gagcaccgcg acggcgtagt cctcggcgct gatgaagctc ttgccctccg cgtctgtgag
     5341 caactggtcg cctcccacac ggtaacggcc cgtgcgctcg cccggtgaga tctccgccgc
     5401 cgggctgagg taggtccagt tcacgtcgga gtcgcggatg acgttcaggg cctcgcgccc
     5461 ctggatggcc tcggccctgt acgcctccgg gaaaccgggc gcgtccagaa gctgcacgcc
     5521 gggcgcgacc tccaggctgc ccgcgccgcc cagtacgaac aggcgtgtca cgctcgcgtc
     5581 acgagcgccg tcaatggcgg cccgcacgac ctccgggacg ggcgcgtcgc ccccgcggcg
     5641 ggcgctgaca gccaccacga ccgcgtcgtg acccgcaaag atgctggcgg ccacgccggc
     5701 gtcccgcacg tcggcctgga cgacccgcac atcctgctca gagctcaacg cgccctcgcg
     5761 acggacgacg gcggttacgt cgtggccgcg cgaaacggcc tcttgcagga tacgggatcc
     5821 cagaattccg gccgcgccga ttatgagtag tttcatacga acccccttgt gtgaagagaa
     5881 ggagcggcga cgcgaaggtc ccgctctcct gctgcgaaac gttttatcta gccgaggacc
     5941 ttcaccgtgc tcccgaagcc ggtgccgagc ggctgctgga gcgtcatgtg caggaacccg
     6001 agcgcttcta gctggcgcgc ccggcgcaag ggaccggcgt cgatgggccg caaacctccg
     6061 gcctcgatca acgagatcac agtctgcttc gcgtctgtat cgtcggcggc gacgaatacg
     6121 tcgagcggct ggccgtccac ttcccctccg acgagcgtgc cggcgaaggt ggtgttgaac
     6181 gccttgacga cggcggattc ggggaggaga ttgccgatct cctcggccgc ggagctgtcc
     6241 ggcggagtga cgagccggtc gaaggactcc cagtcaacgg ggttggtgat gtccacgacg
     6301 accttgcccg cgagcccgga cccgtactgt ccggctatgg actccacggc gccatagggg
     6361 acggcgagga tgacgatgtc gccctcgatg ggatccccga gggatcctgc ctcagccttc
     6421 gcgccgtcta ctccccgcaa ctctcccgca agagcgcccg ccttctcggg gtcccggtct
     6481 atgatgcgga cgctgttccc gccggccacg agccggtcgc cgatcccgcg agccatgttc
     6541 cccgagccga tgatggttac gttcatgatc tctccttact ctttaacgac cgttaccaga
     6601 ggacgatgcc cagcgcggcg tcgagactga tggcgcccgc gccggtgagc agcagcgtca
     6661 gggccatccc ggcgagcgcc aggttgaact cgtaaccgcc gttcatggcg aagaagccgt
     6721 tcttcagatg caccgtgagg gccgcgacgg ccatcgtcac gatcagggcc accgccgcca
     6781 gcggcgtgag gagcccaacc gccaccagta tcccgccagc gaactcggcc agtcccgcga
     6841 ggaccgccat caggtaaggc ggctttatgc ccaactgctc atgaaacccc gccgtaccct
     6901 tcaaccccgg cccaccgaag gaaccgaaca acttctgagc gccgtgggcg gcgaagatca
     6961 caccgagcac aaccctcaat accagcaaac caacatccaa catctgcgac ctccctgggt
     7021 ctttagttac tcaacgtaac caagtataat ttcatatgtt ggttacgtca agtctctact
     7081 cgtgataaaa tgcggttgtg atgattggtt cggatgagat ctat
//
LOCUS       DASSMB010000280        19853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1500825, whole genome shotgun sequence.
ACCESSION   DASSMB010000280 DASSMB010000000
VERSION     DASSMB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 19853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19853
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500825"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFJ72_15840"
     CDS             <1..214
                     /locus_tag="VFJ72_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480977.1"
                     /translation="TLRDLGGTSWVVQGTITEAERRVVRVAAAVVRVAVEAVPVVRAV
                     AADATGTVGAAVEVGSARTPISRTGW"
     gene            208..657
                     /gene="rpsE"
                     /locus_tag="VFJ72_15845"
     CDS             208..657
                     /gene="rpsE"
                     /locus_tag="VFJ72_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET7480978.1"
                     /translation="MVEIRRVAKVVKGGRRFNFSALVVVGDGKGRVGVGLGKANTVPV
                     AISKGQDKAKREMFNVPMRNTTIPHGVIGKYESSEVLLKPASEGTGVIAGGGVRAVLE
                     LAGIKDVLTKALGSTTSVNLVRATVEGLQRLRTKAQIEQQRGVKITS"
     gene            654..845
                     /gene="rpmD"
                     /locus_tag="VFJ72_15850"
     CDS             654..845
                     /gene="rpmD"
                     /locus_tag="VFJ72_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET7480979.1"
                     /translation="MSPTKLKVTQLKSVIGSKEPHKRTIKALGLKRIRDSRVHGDTPQ
                     IRGMLHKVRHLVRVEEVDE"
     gene            845..1306
                     /gene="rplO"
                     /locus_tag="VFJ72_15855"
     CDS             845..1306
                     /gene="rplO"
                     /locus_tag="VFJ72_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7480980.1"
                     /translation="MRLNDLHPAPGSTKERKRLGRGKGSGDGKTSGRGHKGAGARSGA
                     STHPTYEGGQMPLQRRLPRLKGMARGRHTVARPKVYSAVNVGQLEDVSGDTIGLDELR
                     AAGLVGKREGLVKILGDGEIGRSVKVSAHAFSKTAREKIEAAGGSAETLES"
     gene            1309..2589
                     /gene="secY"
                     /locus_tag="VFJ72_15860"
     CDS             1309..2589
                     /gene="secY"
                     /locus_tag="VFJ72_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7480981.1"
                     /translation="MLGALGNAWRVPELRQKLMFTAIMIALYRVGAYVPLPGINRDAI
                     AQGAQSGNAITGLFGAFTGGAFNNFAIFSLGIMPYITAAIVMQLLTVAIPRLQELAKE
                     GETGQQKITQYTRYFTLILALLQSVALALFFRSGQFGPVLADATMIDMFLIVITLTTG
                     VMLTMWIGELITQRGLGNGISLIITASIISRAPSAIKTLYEDGSILNVVILAILAVLV
                     VAAIVFVNEGQRRIPITYAKRQVGRRMSQGGTTYLPLKVNMAGVIPIIFASSLLIFPV
                     VLGQFAAGGNPDSILGSIANFFQPGNAPYLILYALLIVMFTYFYTAVQFNPIEHADNL
                     KKQGGYVPGIRPGQPTALYLNGVLTRITLFGAIFLATVAVLPYLITGVMNLPNSIFLG
                     GTSMLIVVGVSLDTVRQLESQLMMRNYEGFLKKR"
     gene            2586..3221
                     /locus_tag="VFJ72_15865"
     CDS             2586..3221
                     /locus_tag="VFJ72_15865"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET7480982.1"
                     /translation="MRVILLGPQGAGKGTQAHRIAQKTGAKHISTGDIVRAEIKSGSE
                     LGQKVQDYNDRGELVPDEIIVEMAKPYLNDADSWLLDGFPRNESQARALDEALEELGI
                     ELDKAIALEAPDDALVTRLSGRRTSEATGNIYHVKYDPPPEDSDEDPGPFVQRKDDTE
                     DSIRRRLEIYHEQTEPLKDYYAERGLLATVDATQEISKVTDDVLKAVGETA"
     gene            3218..3982
                     /gene="map"
                     /locus_tag="VFJ72_15870"
     CDS             3218..3982
                     /gene="map"
                     /locus_tag="VFJ72_15870"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET7480983.1"
                     /translation="MIVRKSKTELEAMREGGEITAACLKMLAQNVRAGVTTADLDRLA
                     EAFIYDHGGKPEFKGYQGFPASICASPNAMIVHGIPGSYRLKDGDVISLDVGVRYDGF
                     VTDSATTVAVGEISRDADGLLRATRKCLSAATEQMRAGNHLGDIGHAIQSVAEARGYG
                     VVRDLVSHGVGRQMHEDPQIPNYGEPGTGPRLQPGMVFAIEPMITLGSYEIDLDERDG
                     WSIYTADRSLAAHYEHTVAVTENGPLVLTGSGVPGA"
     gene            4087..4308
                     /gene="infA"
                     /locus_tag="VFJ72_15875"
     CDS             4087..4308
                     /gene="infA"
                     /locus_tag="VFJ72_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7480984.1"
                     /translation="MAKEDVIEVEGTVTEALPNTQFRVELESGHNVLAHISGKMRMNY
                     IRILPGDRVKVELSPYDLSRGRITYRFRN"
     gene            4319..4432
                     /gene="rpmJ"
                     /locus_tag="VFJ72_15880"
     CDS             4319..4432
                     /gene="rpmJ"
                     /locus_tag="VFJ72_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET7480985.1"
                     /translation="MKVRASVKPICERCKVIRRRGVVRVICSNPRHKQRQG"
     gene            4434..4805
                     /gene="rpsM"
                     /locus_tag="VFJ72_15885"
     CDS             4434..4805
                     /gene="rpsM"
                     /locus_tag="VFJ72_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET7480986.1"
                     /translation="MARVAGVDLPREKRVEIGLTYVYGVGRSTAKKICRETEVDPNTK
                     VRDLAEGEVAKIRTYIDQNVEVEGDLRRETNQNIRRLIEIGTYRGIRHRRGLPVRGQR
                     TKTNARSRKGPKPSIGGRKKK"
     gene            4806..5228
                     /gene="rpsK"
                     /locus_tag="VFJ72_15890"
     CDS             4806..5228
                     /gene="rpsK"
                     /locus_tag="VFJ72_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET7480987.1"
                     /translation="MGRQRQQRGGQRTRGASRSRRKVRRNITTGVVHIKSSFNNTIVS
                     VSDQEGNVIAWESAGALGFKGSRKSTPYAAQMTAESCANKAMDQGVRRVDIEVKGHGS
                     GRDMAARTFQSMGIEVLSIKDVTGQPHNGCRPPKRRRG"
     gene            5232..5834
                     /gene="rpsD"
                     /locus_tag="VFJ72_15895"
     CDS             5232..5834
                     /gene="rpsD"
                     /locus_tag="VFJ72_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7480988.1"
                     /translation="MARYTGPRGRRDRRAGVMLSSMRKNPLERKPYPPGEHGRGRQRQ
                     TEYGMRLMEKQKARWFYCVSEKQFRKAYDKATRMQGVSGENLLRLMELRMDNVIFRLG
                     FATSRPQARQLVVHGHFQLNGRKHNIPSAILKPGDVITVKEKSRKLEPIENAVENVVA
                     VPAWLEADHDNFTGRLLHAPGREEIDAPVEEQLIIEFYSR"
     gene            5871..6818
                     /locus_tag="VFJ72_15900"
     CDS             5871..6818
                     /locus_tag="VFJ72_15900"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7480989.1"
                     /translation="MLDIAPPRFRVEEEDERRAIFVAEPLPRGLGHTLGNSLRRVMLS
                     GLTGSAVTKIRIEGVQHEFSTVPGVKEDVVDIILNVKGLKFKLEREEPIELEITKDGP
                     GEVTAADIDLKADVEVIDEDHYLASVSEGGHLDLRLTVERGQGYVRAEQNKSDADPIG
                     VIAVDSLFSPVQRVNYSVAETRAGARTDLDSLTLEIFTDGRIEPREALQDASRQLVDI
                     LALFTDGYQGSGGGGEARRGGGRPVIQDERPVEDLELTVRSYNCLKREGVDTIGQLAT
                     MTEEELMNIRNLGMKSVDEIRSKLSEYGYQMESEEYEAR"
     gene            6805..7170
                     /gene="rplQ"
                     /locus_tag="VFJ72_15905"
     CDS             6805..7170
                     /gene="rplQ"
                     /locus_tag="VFJ72_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7480990.1"
                     /translation="MRHAKRGRKLGRDAQHRNLMLGTMAGQVITHGRIKTTAPKAKEL
                     RGVVDKLINTAKKDDLAARRHAVKILKDKTIVRRLFEEVAPELDGRDSGYTRILRLGP
                     RLGDGAEAVYLELVNHSAE"
     gene            7290..8054
                     /locus_tag="VFJ72_15910"
     CDS             7290..8054
                     /locus_tag="VFJ72_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7480991.1"
                     /translation="MDGVGFEVRAGEHVGVVGPNGGGKSTLVRLLNGLLRADSGRVRV
                     MGVDPSVEPFEVRRRLGILFQNPENGLVAPFVEDDVAFGLENAGVPREHMRERVAGAI
                     RAVGLEGYERREPHTLSGGEKQRVALAGLLAVEPEILVLDEPTSMLDAEGRREVLEHL
                     EALRGTKTILHVTHRLEELLDADRVLILNGGRLVAEVTPERLLSDTELLRENRLSLPT
                     VSRIALDLGLPPAMLRTPEELARAIVARTVSETRAR"
     gene            8051..8866
                     /locus_tag="VFJ72_15915"
     CDS             8051..8866
                     /locus_tag="VFJ72_15915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7480992.1"
                     /translation="MRLELKDVRHVYSRGTPFEVEALRGVSLTVEPGEVLGIVGGTGS
                     GKSTLVQHMNLLLAPASGTVLVDGADATSLKKSELRRRVGLVFQFPEAALFAPTVEED
                     VAFAPQRLGLDEEEVRARVRQSLEALGAGHLAGRSPFALSGGEKRRVAIAGVLAMGPE
                     VLVLDEPTAGLDPATREDLLGLVRGLREAGMSVVLVSHDLDEVAEVADRVCLMERGEV
                     RAIGNPAEVFYADPANAPATVQVMAALKETHPGVSEPVRFGETVAALKRLLEH"
     gene            8867..9658
                     /locus_tag="VFJ72_15920"
     CDS             8867..9658
                     /locus_tag="VFJ72_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET7480993.1"
                     /translation="MAASRSIGQFYPVASPLHALDPRAKILATAILVVGVFLIHSIAG
                     FLVVAAAVAVLVTVGRIPALTFLRLLRPVLFIVVLTVFFQVLFSREGATLFGWGIFEV
                     HVGGLRLGLLLALRILLLVSVAGLLTATTAPVALADGIEDLLSPLKRVRFPAHEVAMM
                     MTIALRFIPTLGEEAEKITKAQAARGADFSEGGLLHRVRALVPVLVPLTVGAFRRADE
                     LAEAMESRGYRGGEGRTRYRELRFRARDALALVLTVLILAAGVLA"
     gene            9655..10419
                     /gene="truA"
                     /locus_tag="VFJ72_15925"
     CDS             9655..10419
                     /gene="truA"
                     /locus_tag="VFJ72_15925"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7480994.1"
                     /translation="MKLAGLVEYDGTEFAGWASQPGMRTVEGTLTQALQTVLRQPVKL
                     SVAGRTDAGVHASGQVVSFGADTDLSPSLISYKATAVLPKDLALRRCVPAPQGFDARR
                     SARSRSYEYRVVNHEIRSPLQRHRASYVARSLDFGRLSQTARLVQGIHDFRAFTPSRS
                     YHVRFERIVSESRWERGGDVLTYHITADSFLYGMVRTLVGTMLEVAGEKRDMKDFERL
                     LAGGERSEAGPAVPSRGLTLVGVGYDLDLWGGASDG"
     gene            10412..11086
                     /locus_tag="VFJ72_15930"
     CDS             10412..11086
                     /locus_tag="VFJ72_15930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET7480995.1"
                     /translation="MGEDKTIELLREIDAYVEGLFAPPDEALEAALRDSRAAGLPEIN
                     VSASQGRLLGLLTEISGARRILEIGTLGGYSAIHFARALPDGGAMISLELDERHADVA
                     RRNIERAGLSEKVEILVGDARELLAAMVEAGIEPFDLTFIDADKEGYPEYLDFALQLS
                     RPGSLILGDNTIRGGSVLDPRDGSARATREFNERIARDPRLSSVLLPLIRERIDGLTI
                     ARVKMV"
     gene            11184..11618
                     /gene="rplM"
                     /locus_tag="VFJ72_15935"
     CDS             11184..11618
                     /gene="rplM"
                     /locus_tag="VFJ72_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019394039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7480996.1"
                     /translation="MMKSYMARPQEVERKWYVVDAEGQTLGRLAAEITKVLRGKNKPQ
                     YTPHVDTGDFVVVVNADRVEVTGRKAEQKVYRRHTGYPGGLRETSYEVMMQRKPTEIL
                     RKAVWGMMPKSRLARQQFKKLKIYAGSEHPHAAQTPEPYEVK"
     gene            11620..12012
                     /gene="rpsI"
                     /locus_tag="VFJ72_15940"
     CDS             11620..12012
                     /gene="rpsI"
                     /locus_tag="VFJ72_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7480997.1"
                     /translation="MAEALYTGTGRRKTAVARVRILPGDGKITVNGRDYKEYFPRPAY
                     QTVVSSPLTLLGSASNYDVVAKLEGGGPTGQAEALRHGIARALAEESQEARGELKNAG
                     MLTRDARAVERKKYGLKKARKRPQFSKR"
     gene            12100..13452
                     /gene="glmM"
                     /locus_tag="VFJ72_15945"
     CDS             12100..13452
                     /gene="glmM"
                     /locus_tag="VFJ72_15945"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET7480998.1"
                     /translation="MERRPITFGTDGVRGVANRGLTPEDALRLGLAGARAFGGSILIG
                     RDTRLSGGMLSGALAAGAASGGARVIDLGVLPTPGVAALAPKLGASAAAVVSASHNPY
                     PDNGIKFFSGEGRKLSEEKEREFERLTAEDFERPTGEGIGAVEVLPDAAALYVEGVLE
                     RLRPSAGGMKVLLDCANGAAYEAAPMAFGELGVDLTLSGVEPSGVNINEGCGSTHIGE
                     LDAAGHDVAFAFDGDADRVLAVDERGNVVDGDRILAILARDLMERGKLAGGVVVTVMS
                     NLGFFKAMDSLEIPYEVTPVGDKYVAEAMGRLGASVGGEQSGHVILSEHVTTGDGLVT
                     ALALLDVMSRSGRPLSELARVMEVYPQALINVRVEEPGAAKTVAASEPVERAVSEAEA
                     RLGGEGRILLRPSGTEPVVRVMVEHVDETVCREVCEEVAEAVSRAGMNADRADREVEV
                     "
     gene            13449..14303
                     /locus_tag="VFJ72_15950"
     CDS             13449..14303
                     /locus_tag="VFJ72_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7480999.1"
                     /translation="MRFIGCALAWRPGEAKEKPSCLVVLDERGSIIANSFAGSAQELA
                     SAAEKYTSERRGVLVGIDAPLAVPNERGTRKIERILSKVALPAYSASRKMFGGEPFSE
                     EVLQELEEVGIQYTDYPFPRERGQWAVTEVDSSATLKVLSLERNGQKNGDLVEALRAM
                     PEPKLRKGNKEVRAAAIKSAVDILWNTPGLRLRTGNLSADLTLSENVDLSKLDVDASL
                     SHADLDRVLSLVEGTLAAYTVHRHWRGRDGSMVVGTGDAGSVLLPAGGALHDRIATEC
                     RAAKVPFV"
     gene            14305..15069
                     /gene="hisF"
                     /locus_tag="VFJ72_15955"
     CDS             14305..15069
                     /gene="hisF"
                     /locus_tag="VFJ72_15955"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET7481000.1"
                     /translation="MGLKVRIIPCLDVDAGRVVKGTQFKDLRDAGDPVELAALYDREG
                     ADEIVFYDITASHEARNTATALARRAAEEVFIPYTIGGGVRTVEDMRDMLRAGADKVS
                     INSAAVRTPDLIDSGAERFGSQCVVLSVDVKRRAPGAKAGWEVYLNGGRVNTGMDAVE
                     WLVEGERRGAGEFVLNSMDSDGMESGYDLDLISAVAERTSVPIIASGGAGSPEHMIAA
                     VNAGAGAVLAASIFHFGEWSIREVKEHMTQAGIPVR"
     gene            15066..15707
                     /gene="hisIE"
                     /locus_tag="VFJ72_15960"
     CDS             15066..15707
                     /gene="hisIE"
                     /locus_tag="VFJ72_15960"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HET7481001.1"
                     /translation="MSAAERVRFDQNGLVPVIAQDAHTGEVLTLAYANREAVEKTLSS
                     GEAHYYSRSRAELWRKGATSGNTQKVVEVRLDCDGDALLYRVEPRGPACHTGERTCFF
                     TALAEEGVGLAMGEAGAPEGFGAMVEHLAGTIAERHREMSEESYTAALIRRGTPRVAQ
                     KVGEEAVEVVVAALQDDRLAEETADLVYHLLVLLEERGVGIDEVARVLRERHR"
     gene            15691..17211
                     /gene="trpE"
                     /locus_tag="VFJ72_15965"
     CDS             15691..17211
                     /gene="trpE"
                     /locus_tag="VFJ72_15965"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET7481002.1"
                     /translation="MNVTARSLTPTLDEARRLARTHDVVPVYAEFIGDLETPISAVLK
                     FAGEENVFLLESAESAERFGRYSFLGFDPKRTLSYRNGIYTVVDADGVREVPAKDPFR
                     GLAEIVGKKSVAPLPNLPAFVGGAVGYFSYDAVRYLERLPDAPPDELDVPEAYFAVTD
                     TLVVFDHLRHKVLVISLVDASLLRDVEGEGFSAAYRRAADDVRRVAERLSAPLVRRAL
                     PDGAGGSFEVTSNFTEAGYVEAVEKAKEYIRAGDAFQIVPSQRFQAGIGDLDPLLLYR
                     GLRTVNPSPYMTYLKLGDLAIVGASPEPLVRVEGRRVMTRPVAGTRPRGGTPEEDAAL
                     AEELLADEKERAEHVMLVDLGRNDLGRVSEIGSVELESFMEIERYSHVMHIVSTVEGN
                     LREDLTALDALAAAFPAGTVSGAPKVRAMEIIDELEPTRRGPYAGATGYYGIDGRLDT
                     CITLRTALLKNETAYFQAGGGVVADSVPKLEYEESRNKARAMARALEVARSREMWL"
     gene            complement(17246..17422)
                     /locus_tag="VFJ72_15970"
                     /pseudo
     CDS             complement(17246..17422)
                     /locus_tag="VFJ72_15970"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
     gene            complement(17541..18386)
                     /locus_tag="VFJ72_15975"
     CDS             complement(17541..18386)
                     /locus_tag="VFJ72_15975"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7481003.1"
                     /translation="MTQDDVPAAYRAGSIALTESAEEAERARNRSAEEVARREARYRH
                     ALEHDPGGAWVAVDGDRVAGAALALVREGVWILSLLAVDEAYRNAGVGRDLLDHALRY
                     AAGCEGAMIAASTHPAALRRYALAGFTLLPTLMASGTIRREALPADPSVRDGEKQDLE
                     LATEVDRKLRGAAHGPDLELMLRAGARLFVAEGPAGRGYAVEYEGHPAIVAATAPEVA
                     GDLLWACLAECTGETEVRWLTGSQDWAVRVALDAGLSLSPQGPVCVRGELGPLTPYLP
                     SGAFL"
     gene            18477..19562
                     /locus_tag="VFJ72_15980"
     CDS             18477..19562
                     /locus_tag="VFJ72_15980"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET7481004.1"
                     /translation="MFREKSERKSRRRHLGVWDPTGGEAREGSGLPVEEGVEKIRILA
                     RDVDGGVREVRAEELEGAMAGAAVWIDLVRPRERAERILRDALKLPPLTVEDCLGPLR
                     MPKMDVFTVDGARGGFVAGFAARFERAEMEVGLRAVEVDLVAGPGYLVTVRDGPVPEV
                     GERLERRMADKDLTAQPGFALAYEAMDALIDGHLPALVEAATAAEELDEALDPSDERA
                     SVAALEALISLRRDLQAFRRLATAQQEILRRLGRAAPDLREYLSDAADNQREAVDMAE
                     ATRDYAEGAVEAYRIRRDERSELGIRRLTVFAAILGPLTLVSGIYGANFARIPGTESP
                     LGFPIFVIGQALFVVAAVWFLYRRGLL"
     gene            19595..>19853
                     /locus_tag="VFJ72_15985"
     CDS             19595..>19853
                     /locus_tag="VFJ72_15985"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HET7481005.1"
                     /translation="MVTVFLVLLGYVLGSVPTGYLVGRAFGVDVRQVGSGNIGTANVL
                     RAAGKWPAVFTLAGDMAKGLLPVVVARISTDNVWVHALVAFA"
BASE COUNT         3512 a         5989 c         7170 g         3182 t
ORIGIN      
        1 aactctaaga gatttaggag gaacatcttg ggtcgtccaa ggaacgataa cagaggcgga
       61 gcgccgggtg gtccgggtgg ccgcggcggt ggtccgggtg gccgtggagg cggtcccggt
      121 ggtcagggcg gtcgcggcgg acgcgaccgg gacggtcggg gcggccgtgg aggtcggcag
      181 cgcgagaact ccgatttcca ggaccgggtg gtagagatcc ggcgtgtcgc gaaggtcgtc
      241 aagggcggcc ggcggttcaa cttctcggcg ctggtcgtcg tgggtgacgg caagggccgc
      301 gtcggtgtcg ggctcggcaa ggcgaacacg gtccccgtgg ctatctcgaa gggccaggac
      361 aaggccaagc gcgagatgtt caacgtcccg atgcggaaca ccacgatccc gcacggcgta
      421 atcggcaagt acgaaagttc cgaggtgctc ctgaagccgg cctccgaggg tacgggggtt
      481 atcgcgggcg gcggcgtccg ggcggtgctg gagctggcgg ggataaagga cgtgctcacg
      541 aaggcgctcg gttcgaccac ctcggtcaac ctggtccggg cgacggtcga gggtctgcag
      601 aggctccgca cgaaggccca gatcgagcag cagcgggggg tgaagatcac gtcatgagcc
      661 caacgaagct aaaggttacg cagctaaaga gtgttatcgg ctcgaaggag ccgcacaagc
      721 ggacgatcaa ggcgctggga ctcaagcgca tccgggactc gcgggttcac ggtgatacgc
      781 cccagattcg cggcatgctc cacaaggtgc gccacctggt ccgtgtcgag gaggtggacg
      841 agtagtgagg ctgaacgacc tgcatccggc cccaggctcc actaaggagc gcaagagact
      901 cggacggggc aagggctccg gagacggcaa gacgagcggc cggggccaca agggcgccgg
      961 ggctcgctcc ggcgcatcca cgcacccgac ctacgagggt ggccagatgc cgctccagcg
     1021 ccgcctgccg cggctgaaag gcatggcccg cgggcggcac accgtcgcga ggccgaaggt
     1081 ctactcggcg gtcaacgtcg ggcaactcga agacgtatcc ggtgatacga tagggctcga
     1141 tgagctgcgg gccgccgggc tcgtcgggaa gagggaaggg ctggtcaaga tcctcgggga
     1201 cggggagatc ggacgctccg tgaaagtctc ggcccacgcc ttctcaaaga cggccaggga
     1261 gaagatcgag gcggccggcg ggagcgccga gacactggag tcgtagatat gctaggagcc
     1321 ctcggaaatg cgtggcgcgt tccggaactg cgccagaaac tgatgttcac ggcgatcatg
     1381 atcgccctgt accgggtcgg ggcctacgtg cccttgccgg gtatcaaccg ggacgccatc
     1441 gcgcagggcg cccagagcgg caacgccata accgggcttt tcggggcttt taccggcggg
     1501 gcgttcaaca acttcgccat cttctctctg ggcatcatgc cgtacatcac tgctgctatc
     1561 gtcatgcagt tgctcaccgt ggcgataccg aggcttcagg aactggcaaa agaaggcgag
     1621 accgggcagc agaagatcac gcagtacacc cggtacttca cgctaatcct ggcgctcctt
     1681 cagtcggtgg cgctggcgct cttcttccgc tccgggcagt tcgggccggt gctcgcggac
     1741 gcgacgatga tagacatgtt cctgatcgtg atcacgctga cgaccggcgt gatgctgacg
     1801 atgtggatcg gggagttgat cacccagcgg ggcctcggaa atggtatctc gctgatcatc
     1861 acggcttcga tcatctcccg cgctccgagc gcgataaaga cgctctacga ggacgggagc
     1921 atcctgaacg tcgtcatact cgccatactg gcggtcctcg tggtggcggc catcgtgttc
     1981 gtaaacgagg gccagcggcg catcccgatc acctacgcca agcggcaggt cggacgccgg
     2041 atgagccagg gcgggaccac ctacctgccg ctgaaggtga acatggcggg cgtcatcccg
     2101 atcatcttcg cctcgtcgct cctgatcttc ccggtcgtgc tggggcagtt cgcggcgggc
     2161 ggcaacccgg actcgatcct cggcagcatt gccaacttct tccagccggg caacgcgcca
     2221 tacctgatcc tgtacgcctt gctgatcgtg atgttcactt acttctacac ggcggtccag
     2281 ttcaatccaa tagagcacgc cgacaacttg aagaagcagg gcggttatgt gccgggcatc
     2341 cgtccgggac agccgacagc gttgtatctc aacggggtat tgaccaggat tacgctcttc
     2401 ggcgcgatct tcctggcgac ggtggcggtg ctgccgtatc tgatcacggg ggtcatgaac
     2461 ctgccgaact cgatcttcct gggtggtacc tcgatgttga tcgtggttgg cgtctcgctg
     2521 gatacggtcc gccaacttga gagtcagcta atgatgcgca actatgaagg gttcttgaag
     2581 aaacgatgag agttatactg cttggccccc agggggctgg aaaagggaca caagcgcacc
     2641 gcatcgcaca gaagacgggc gcgaaacata tatccaccgg cgacatcgtg cgggccgaga
     2701 taaagagcgg ctcagagctc ggacagaagg tccaggacta caacgaccgg ggcgagctgg
     2761 tgccggacga gatcatcgtc gagatggcga agccgtacct gaatgacgcc gactcctggc
     2821 tgctggacgg cttcccgcgc aacgagtcgc aggcgcgggc gctcgacgag gcgctggagg
     2881 agctcggtat cgagttggac aaggccatcg cgctggaggc gccggacgac gctctcgtca
     2941 ccaggctctc cggtcggagg actagcgagg cgacgggcaa catctatcac gtgaaatacg
     3001 acccgccgcc ggaggacagc gacgaggacc ccggtccctt cgtgcagcgt aaggacgaca
     3061 ccgaggactc cattcggcgc aggctggaga tctatcacga gcagaccgag ccgctgaagg
     3121 actactacgc cgaacgcggc ctgctcgcca ccgtggacgc cacgcaggag atctccaaag
     3181 tcaccgacga cgtcctgaaa gccgtgggag agactgcttg atcgtccgca agagcaagac
     3241 cgagctggaa gcgatgcgcg agggcggcga gatcacggcc gcctgcctga agatgctcgc
     3301 gcagaacgtc cgggccggcg tgactaccgc cgacctcgac cggctcgccg aggcgtttat
     3361 ctacgatcac ggcggcaagc cggaattcaa gggctaccag ggcttcccgg cttccatctg
     3421 cgcctcgccg aacgcgatga tcgtccacgg catccccggc tcgtaccgcc tgaaggacgg
     3481 agacgtgatc tctctggacg tgggcgtccg ttacgatggc ttcgtcaccg actcggccac
     3541 gacggtcgca gtaggcgaga tctcccgtga tgccgatgga ctgctgcgcg cgaccaggaa
     3601 gtgcctctcc gccgcgacgg agcagatgcg ggcgggcaac cacctcggcg acatcggaca
     3661 cgcgattcag tccgtggcgg aggcgcgggg ctacggagtg gtgcgggacc tcgtctcgca
     3721 cggcgtcgga cgccagatgc acgaggaccc ccagatcccg aactacggcg agcctggcac
     3781 gggtccgagg ttgcagccgg gaatggtctt tgcgatagag cccatgatca cgctcggcag
     3841 ctacgagata gatctggacg agcgcgacgg ctggtccata tacactgctg accgatccct
     3901 cgccgcccac tatgaacaca ccgtcgccgt caccgagaac gggccgctgg tactcactgg
     3961 aagcggggtg cccggagcgt aagttttgcc cagagggatt gggaaaatcg gggctggcgt
     4021 ggtagcatat tcgtttggct ccagccactt tgtaacttgt aacgaagaga gagtttgagg
     4081 aggtccttgg ctaaggaaga tgtaatagag gtagagggga cggtgacgga ggcgttgccg
     4141 aacacccagt tccgggtcga gctggagagc gggcacaacg ttctcgcgca catctcgggc
     4201 aagatgcgga tgaactacat ccggatactg ccgggagacc gggtgaaagt agagttgagc
     4261 ccctatgacc tcagccgggg acgtattact tacaggttca ggaactaagg agagcgttgt
     4321 gaaggtcaga gcgagtgtga aaccgatatg cgagcggtgc aaggtgatcc gccggcgtgg
     4381 cgtggtgcgg gtgatctgct ccaacccccg gcacaagcag aggcagggtt agtatggcgc
     4441 gcgtagccgg cgttgacctg cccagggaga agagggtcga gatagggctc acctacgtct
     4501 acggcgtcgg gcgttcgacg gccaagaaga tctgccgcga gaccgaggtg gacccgaaca
     4561 cgaaggtccg ggacctggcc gagggcgagg tcgccaagat ccggacctac atcgaccaga
     4621 acgtcgaggt cgagggcgac ctcaggcgtg agacgaacca gaacatccgg cggctcatag
     4681 agatcgggac ctaccggggc atccggcatc gccggggcct cccggtgcgc ggacagcgga
     4741 cgaagaccaa cgcccgctcg cgcaagggtc cgaagccgtc cataggcggg aggaagaaga
     4801 agtagatggg taggcagagg cagcagcgcg gcggccagcg cacccgtggg gccagtcgca
     4861 gccggcgcaa ggtccgcagg aacattacga ccggcgtcgt ccacatcaag agctcgttca
     4921 acaacaccat agtcagcgtc tccgaccagg aaggcaacgt catcgcctgg gaatcggcgg
     4981 gcgctctggg cttcaagggg agccgcaaga gcacgcccta cgccgcccag atgacggccg
     5041 agagctgcgc gaacaaggcg atggaccagg gcgtgcggcg cgtggacatc gaggtcaagg
     5101 gccacggctc gggccgcgac atggccgcga ggacattcca gtcgatgggc atagaggtcc
     5161 tctcgataaa ggacgttacc ggccagccgc acaacggatg ccggccgccc aagaggcgta
     5221 gggggtagct gatggcgcga tacacgggac caagaggacg cagagaccgc agggccgggg
     5281 tgatgctctc ctcgatgcgg aagaacccgc tggagaggaa gccctacccg cccggtgagc
     5341 acggccgcgg caggcagcgt cagacggagt acgggatgcg gctcatggag aagcagaagg
     5401 cccgctggtt ctactgcgtc tccgagaagc agttcaggaa ggcctatgac aaggcgaccc
     5461 ggatgcaggg cgtctccggc gagaacctct tgcggctcat ggagctccgg atggacaacg
     5521 tgatcttccg cctcggcttc gcgacgagcc ggccgcaggc taggcaactc gtggttcacg
     5581 ggcacttcca gctcaacggc cgcaagcaca acatcccttc ggccatcctc aagccgggcg
     5641 acgtcatcac ggtcaaggag aagagccgga agctggagcc catcgagaac gccgtcgaga
     5701 acgtcgtggc tgttccggcg tggctggagg ccgaccacga taacttcacg ggccggctgc
     5761 tgcacgctcc gggccgggag gagatcgacg ctcccgtaga agagcagctc attatcgagt
     5821 tctacagccg ttaatagaac aactcatatt cagcaggtgg taatttgacg atgttagaca
     5881 tagcgccacc ccgtttcagg gtggaagaag aagacgagcg ccgggcgatc ttcgtggcgg
     5941 agcctctgcc acgcgggctc ggccatactc tgggtaactc gctgcggcgg gtgatgctct
     6001 ccgggctgac gggttctgcc gtcaccaaga tccggataga gggcgtccag cacgagttct
     6061 cgaccgtccc cggcgtcaag gaagacgtcg tggacattat cctcaacgtg aagggcttga
     6121 agttcaagct ggagcgcgag gagccgatag agcttgagat aacgaaggac ggtcccggcg
     6181 aggtcaccgc cgccgacatc gacctgaagg ccgacgtcga ggtgattgac gaggatcact
     6241 atctggcgag tgtctcggag ggtggacacc tggacctgcg cctgacggtc gagcgcggcc
     6301 agggctacgt gcgcgccgag cagaacaaga gcgacgcgga ccccatcggc gtcatcgcgg
     6361 tggactctct attctcgccg gtgcagcgcg tgaactactc ggtagcggag acccgcgccg
     6421 gggcgaggac cgacctcgac tccctgacgc tggagatctt tactgacggc cgcatcgagc
     6481 cgcgggaggc tttgcaggat gcctccaggc agctcgtgga catcctggca ctgtttacgg
     6541 acggctacca gggctccggc ggcggtggcg aggcgcgccg gggaggcggc aggccggtaa
     6601 tccaggacga gcgaccggtc gaggacctgg agctcacggt ccggtcgtac aactgtctca
     6661 agcgcgaggg cgtggacacc atcggccagc tcgccaccat gaccgaggag gagctgatga
     6721 acatccgcaa cctcggcatg aagagcgttg atgagatccg gtcgaagctc tccgagtacg
     6781 ggtaccagat ggagagtgag gaatatgagg cacgctaaga gaggacgcaa gctcggccgc
     6841 gacgcccagc accgcaacct gatgctcggc accatggccg ggcaggtcat cacgcacggc
     6901 cgcataaaga ccaccgcccc gaaagccaag gagcttcgcg gcgtcgtgga taagctcata
     6961 aacacggcca agaaagatga cctggcggcc cggcggcacg cggtcaagat cctgaaggat
     7021 aagaccatcg tccgccggct ctttgaggag gtcgcccccg agcttgacgg ccgcgactcc
     7081 ggctacacac gcatccttcg gctgggaccg cgtctcggtg acggtgccga ggcagtctac
     7141 ctggagctgg tcaaccactc cgcggagtaa ccgtaacgtc acacacggat aaacccgcca
     7201 cagggacccg cccggcgcga gccagcgggt ccctcgccgt ttcctgcgat aacctctctt
     7261 actcctatcc cggaacggaa cgcccggcgc tggacggcgt cgggttcgag gtccgggccg
     7321 gagagcatgt cggcgttgtt gggcccaacg gcggcggaaa gtctaccctg gtacgtctcc
     7381 tgaacgggct gctccgggca gactccggcc gcgttcgcgt gatgggtgtc gatccttccg
     7441 tggagccgtt cgaggttcgc cggaggctcg gaatcctgtt ccagaacccg gagaacggtc
     7501 tggtcgcgcc gtttgtagag gacgacgtgg cgttcgggct ggagaacgcg ggcgtaccgc
     7561 gggaacacat gcgggagcgg gtcgccggag cgatacgggc ggtcggcctt gaaggctacg
     7621 agcgccgcga gccgcacaca ctctcgggcg gcgagaagca gcgtgtcgct ctcgcgggac
     7681 tcctggcagt ggaacccgag atcctcgtcc tcgacgaacc tacctcgatg ctcgacgccg
     7741 agggacgccg cgaggtgctc gaacacctcg aagctctgcg cgggacgaag acaatcctgc
     7801 acgtaaccca tcgcctggaa gaactactcg acgcggaccg cgtcctgatc ctgaacggcg
     7861 gaaggctcgt ggcggaggtg acgccggagc ggttgctgtc cgatacggag ttgctgcgcg
     7921 agaaccggct ctctctcccg accgtgtcgc ggatcgccct ggacctcggt ctgccgcccg
     7981 cgatgcttcg tacgccggag gagcttgcgc gagcgatagt ggcgcgaacg gtgagcgaga
     8041 cgagggcgcg atgaggctgg agttgaaaga tgtacgtcac gtctactcgc gaggaacgcc
     8101 cttcgaggtc gaggcgttgc gcggcgtgtc gctcaccgtg gagccgggag aggtgctggg
     8161 tatagtcggt gggacaggct ctgggaagag cacgctcgtt cagcacatga acctgcttct
     8221 cgccccggcg agcgggacgg tgctcgtgga cggcgcggac gcgacgtcgc tcaaaaagag
     8281 cgaactgcgg cgcagggtgg ggctggtgtt ccagttcccg gaggccgctc tcttcgcgcc
     8341 gacggtggag gaggacgtgg ccttcgcgcc gcaacggctc gggctggacg aggaggaggt
     8401 gcgggcaagg gtccgccagt cccttgaggc gctgggagcc gggcatctgg ccggacgttc
     8461 gccgttcgcg ctctccgggg gagagaagcg gcgggtagcc atcgctggtg tgctcgcgat
     8521 gggaccggag gtcctggtcc tcgacgagcc gaccgctggt ctcgacccgg ccacgaggga
     8581 ggacctgctc gggctggtga ggggtctgcg ggaggcgggt atgtcggtgg tgctcgtctc
     8641 tcacgacctg gacgaggtcg cggaggtcgc ggaccgggtg tgcctgatgg aacggggcga
     8701 ggtccgggct atagggaatc cggcggaggt gttctacgcg gacccggcga acgccccggc
     8761 taccgtacag gttatggctg cgctgaaaga gacgcatccg ggcgtgagcg agccggtgcg
     8821 cttcggggag acggttgccg cattgaagag actattggag cattaggtgg cggcgagccg
     8881 gagcatcggg cagttctatc cggtggcgtc gccgctgcac gcgctggacc cgagggccaa
     8941 gatcctggcg acggcgatac tggtcgtcgg ggtgttcctg atccactcga tagccgggtt
     9001 cctcgtggtc gcggcggcgg tcgcggtcct cgtgacggtg ggccgcatac cggcgcttac
     9061 tttcctgcgg ctcctgcggc cggtgctgtt catcgtggtc ctgacggtgt tctttcaggt
     9121 gctcttctcg cgcgaggggg cgacgctctt cgggtggggg atattcgagg ttcatgtggg
     9181 cgggctgcga ctggggttat tgctcgcgct ccggatactg ctcctggtct cggtagccgg
     9241 gctgcttacg gcgacgacgg cgccggtcgc cctggcggac ggcatcgagg acctgctctc
     9301 tccactgaag agggttcggt tcccggccca tgaagtagcg atgatgatga cgatagcgtt
     9361 gcgtttcatc ccgacgcttg gtgaggaggc cgagaagatc acgaaagctc aggccgcccg
     9421 cggcgcggac ttctccgagg gcggcctcct gcaccgggtc cgggcgctcg tcccggtcct
     9481 ggtccctctc accgtcgggg cctttcggcg cgcggacgag cttgcggagg ctatggagag
     9541 ccggggctac cggggcggcg agggacgcac ccgataccgc gagctgcgtt tccgcgcccg
     9601 cgacgccctg gctctcgtcc tcaccgtgct cattctcgcg gcgggagtgc tggcgtgaag
     9661 ctcgccgggt tagtggagta cgatgggacg gagttcgccg ggtgggcatc gcagccgggg
     9721 atgaggaccg tcgagggtac actcacgcag gcgctccaga ccgtcctgcg ccagccggtg
     9781 aagctgagcg tggccgggcg cacggacgcg ggggtgcacg cgagcgggca ggtcgtctcg
     9841 ttcggcgcgg acacggacct ttcgccatcc ctgatctcct acaaggccac ggccgtgctg
     9901 ccgaaagacc tggcgctccg gcgctgcgtc ccggcaccgc aagggttcga tgcccgacgt
     9961 agcgcccgga gccggagcta cgagtatcgg gtggtgaacc atgagatccg gtctccactg
    10021 caacgccacc gggccagcta cgtcgcccgc tcgctggact tcggccgcct ttcgcagacg
    10081 gcgaggcttg tccagggcat acacgacttc cgggcgttca ctccgtcgag gagctaccac
    10141 gtccgcttcg agcggatagt ctcggagtcg cgctgggagc gtggcggaga cgtcttgact
    10201 taccatataa ccgccgactc gttcctgtac gggatggtgc ggacgctggt cgggacgatg
    10261 cttgaggtgg ctggcgaaaa gcgtgacatg aaggacttcg agcggctgct ggcgggtgga
    10321 gagcggagtg aggcggggcc ggccgtaccg agcaggggcc tcacgctcgt cggtgttggc
    10381 tatgacctgg acctctgggg aggagcgagc gatgggtgag gacaagacga tagagcttct
    10441 gcgggagata gacgcctacg tggagggact cttcgcgccg ccggatgagg cgctggaggc
    10501 ggctctgcgg gactcgcgag ctgccgggct gccggagatc aacgtctcgg cgagccaggg
    10561 gcggctgctc gggctgctca cggagatctc gggtgcgcgg cggatactgg agatcgggac
    10621 tctcggagga tacagcgcga tccacttcgc ccgcgccctg ccggacgggg gagccatgat
    10681 ctcattggaa ctcgacgaac gccacgccga tgtcgcccgc cgcaacatcg agcgcgccgg
    10741 tctctcggag aaggtcgaaa tactggtggg ggatgcgcgg gaactactcg cggcgatggt
    10801 cgaagcagga atagaaccgt tcgacctgac cttcatagac gccgacaagg agggctatcc
    10861 cgagtacctg gacttcgcgc tccagctctc gcgtcccggc tcactcatcc tcggcgataa
    10921 caccatccgc ggcggcagtg tgctggaccc gcgggacggc tccgcgcggg cgacgcggga
    10981 gttcaacgag cggatagccc gagatcccag gctctcgtcc gtcctgctgc ccctgatccg
    11041 ggagcgcata gacggtctca ccatcgcccg cgtaaagatg gtctgagagg agggaatttc
    11101 ccatagattc tgaccgtatt gtagatatat gctactattt gcagcgtttg gaattccgtt
    11161 gaactttgca aggactccgg aggataatga agagttatat ggcgcggccc caggaggtcg
    11221 agcggaagtg gtatgtcgtt gacgcggagg ggcagaccct cgggcgtctg gcggcggaga
    11281 taacgaaggt cctgcgcggc aagaacaagc cgcagtacac accgcacgtg gacactggcg
    11341 acttcgtggt cgtggtgaac gcggatcggg tcgaggtgac gggccgcaag gcggagcaga
    11401 aggtttaccg gcggcacacg gggtatccgg gtggtctccg ggagaccagc tacgaggtta
    11461 tgatgcagcg caagccgacc gagatcctcc gtaaggccgt ctgggggatg atgccgaaga
    11521 gccggctcgc ccgccagcag ttcaagaagt tgaagatcta cgccgggtcc gagcatccgc
    11581 acgccgctca gacccccgaa ccttacgagg tgaagtagca tggcagaagc tctgtatacg
    11641 gggaccgggc ggcgcaagac cgccgtggcg agggtccgca tcctgccggg agacggcaag
    11701 atcacggtta acggccgcga ttacaaggag tatttcccgc gcccggccta ccagaccgtg
    11761 gtctcctcgc cgctgacgct cctgggctcc gcgagcaact acgacgtggt ggcgaagctc
    11821 gaagggggcg gcccgacggg acaggccgag gccctgcggc acggcatcgc ccgcgctctg
    11881 gccgaggagt cgcaggaggc ccgcggcgag ctcaagaacg ccgggatgct gacccgcgac
    11941 gcccgagccg tggagcggaa gaagtacggt ctcaagaagg cccgcaagcg cccgcagttc
    12001 tccaagcgct aggcaagcgc caggccgcgc aaaccgcttt attacggtcg gacccctttc
    12061 gtcgcccgga aggggtccgc tgtctctcta ggaggttgtt tggagcgtag accgatcact
    12121 ttcggcaccg atggcgtgcg cggcgtcgcg aaccgcggac tgacccccga agacgcgcta
    12181 cgcctcggac tggccggcgc gcgggccttc ggcggctcca tcctgatagg ccgcgacact
    12241 cgcctctccg gcgggatgct ctcgggcgct cttgccgccg gagccgcgag cggcggagcc
    12301 agagtaatag acctcggcgt gctgccgaca ccgggcgtcg ccgcgctggc gcctaagctc
    12361 ggcgcctcgg ccgccgccgt tgttagcgcc tcgcacaatc cgtacccgga caacggtatc
    12421 aagttcttct ccggcgaggg ccgcaagctc tcggaggaga aggagcgcga gttcgagcga
    12481 ctgaccgccg aggactttga acgtccgacc ggggagggca tcggggcggt ggaggttctg
    12541 cccgacgccg cggctctgta cgtcgagggt gtgctggaac ggctgcggcc gtccgccggt
    12601 gggatgaagg tcttgctgga ctgcgcgaac ggggcggcct acgaggcggc gccgatggcg
    12661 ttcggggagc tgggcgtgga cctcacgctc tccggcgtcg agccgagtgg cgtgaacatc
    12721 aacgaggggt gcgggtccac gcatataggg gagctggacg ctgccgggca cgacgtggcg
    12781 ttcgccttcg acggggacgc cgaccgggtg ctggctgttg atgagcgggg gaacgtggtg
    12841 gatggggaca ggatcctcgc catcctcgcc cgcgacctca tggagcgcgg caagctggca
    12901 ggcggcgtgg tcgtcaccgt gatgagcaac ctggggttct tcaaggcgat ggactctctg
    12961 gagatcccct acgaggttac gccggtcggg gacaagtatg tcgcggaggc gatgggacgg
    13021 ctcggagcgt cggtcggcgg ggagcagtcg ggccacgtta tcctctcgga gcatgtgacg
    13081 accggggacg ggctggtcac ggcgctggct cttttggacg tgatgtcgcg gagcggccgg
    13141 ccgctctccg agcttgcgcg ggtgatggaa gtgtatccgc aggccctgat caacgtgcgg
    13201 gtagaggagc ccggcgcggc gaagacggtg gcggcatcgg agccggtcga gagggctgtg
    13261 tcggaggctg aagccaggct tggcggggag ggacgtatcc tgctgaggcc gagcggaacg
    13321 gagccggtgg tgcgggtgat ggtcgagcac gtggacgaga cggtgtgccg ggaggtctgc
    13381 gaggaggtcg cggaggccgt ctccagagcc gggatgaacg cggacagagc cgatagggag
    13441 gtcgaggttt gaggtttatt ggctgcgcgc tggcgtggag gccgggcgag gcgaaggaga
    13501 agccgtcgtg tctggtcgtg ctggacgagc gggggagcat catcgccaac tcgttcgcgg
    13561 ggagcgcgca ggagctggcg agcgccgccg agaagtacac ctcggagcgg cggggggttt
    13621 tagtggggat cgacgcgccg ctcgcggtcc cgaacgagcg agggacgcgg aagatcgagc
    13681 gaatcctctc gaaggtcgcg ttgcccgcct actcggcgag ccgcaagatg ttcggtggcg
    13741 agcctttctc cgaggaggtc ctgcaggagc ttgaggaggt cggtattcag tacacggact
    13801 atcctttccc cagggagcgg ggacagtggg cggtgacgga ggtggattct tccgcgacgc
    13861 tgaaggtcct ctccctggaa cgcaacggtc agaagaacgg agacctggtg gaggccctgc
    13921 gggcgatgcc ggagccgaag ctccgcaagg gcaacaagga ggtccgggcg gcggcgatca
    13981 aatccgccgt ggacatcctg tggaacacgc cggggctccg gctgcggacg ggcaacctct
    14041 cggcggacct caccctctcg gagaacgtgg acctctcgaa actggacgtt gacgcctcgc
    14101 tctcgcacgc ggaccttgac agggtcctga gcctcgtgga aggcacgctc gcggcctaca
    14161 cggtccaccg gcactggagg ggacgcgacg gctcgatggt cgtggggacg ggcgacgcgg
    14221 gctcggtctt gctgccagcg ggcggggcac tgcacgaccg gatcgccacc gagtgccgcg
    14281 cggcgaaggt gccgttcgtc tagcatgggg ctcaaggttc gcataatccc gtgtctggac
    14341 gtggacgctg gccgggtcgt gaagggcacg cagttcaagg acctgcgcga cgcgggagac
    14401 ccggtggagc tggccgccct ctacgaccgg gagggggccg acgagatcgt cttctacgac
    14461 atcaccgcct cccatgaggc ccgcaatacg gcgaccgccc tggcccgccg cgccgccgag
    14521 gaggtcttta tcccctatac catcggcggc ggcgtgagga ccgtcgagga catgcgcgac
    14581 atgctgcgcg ccggggcgga caaggtctcc atcaacagcg ccgccgtgag gacgccggac
    14641 cttatagaca gcggcgccga gcggttcggc agccagtgcg tcgtcctgag cgtggacgtg
    14701 aagcgccggg cgcccggagc gaaagccggc tgggaggtct acctgaacgg cgggcgggtg
    14761 aacaccggga tggatgccgt ggagtggctc gtcgaggggg agcggcgcgg ggccggggag
    14821 ttcgtgctca acagcatgga ctccgacggg atggagagcg ggtacgacct ggacctcatc
    14881 tcggcggtcg cggagaggac cagcgtgccg atcatcgcgt cgggcggcgc ggggagcccg
    14941 gagcacatga tcgccgcggt caacgccggg gccggggcgg tcctcgccgc gagcatcttc
    15001 cacttcgggg agtggagcat aagggaggtc aaggagcaca tgacgcaggc cgggatcccg
    15061 gtccgatgag cgcggccgaa agagtccggt tcgaccagaa cggcctcgtc cccgtaatcg
    15121 cccaggacgc ccacaccggc gaggtgctga cgctcgccta cgcgaaccgc gaggccgtcg
    15181 agaagacgct ttcgagcggc gaggctcact actactctcg ctcccgcgcc gagctgtggc
    15241 gcaagggggc gacgagcggc aacacccaga aggtcgtcga ggttcgcctg gactgcgacg
    15301 gcgacgcgct gctctaccgg gtcgagcccc gcggtccggc ctgccacacc ggggagagga
    15361 cctgcttctt taccgctctg gccgaagaag gcgtgggcct cgccatgggt gaggctggcg
    15421 ctcccgaggg tttcggggcg atggtggagc atttggcggg gacgatagcc gagcggcacc
    15481 gggagatgtc cgaggagtcg tacacggcgg ccctgatccg tcgcggcacg ccccgcgtgg
    15541 cgcagaaggt cggcgaggag gctgtggagg tcgtggtcgc cgccctgcaa gacgaccggc
    15601 tggccgagga gacggcggac ctcgtctatc acctgctggt gctcctggaa gagcgcggcg
    15661 tcggcattga cgaggtggcg agggtactgc gtgaacgtca ccgctaggag cctcaccccg
    15721 acgctagacg aagcgcgcag gctcgctcgc acccacgacg tggtgcccgt ctacgcggag
    15781 ttcatcggcg acctggagac cccgatctcc gccgtcctca agttcgcggg cgaggagaac
    15841 gtcttcctgc tggagagcgc cgagtcagcg gagcgcttcg gacgctactc gttcctcggc
    15901 ttcgatccca agcgcaccct ctcctaccga aacggaatct acacggtggt ggacgccgac
    15961 ggcgtgcggg aggtcccggc gaaggacccg ttccggggct tggcggagat cgttggtaag
    16021 aagagcgtgg cgcccctgcc gaacctgccg gcctttgtcg gtggcgcggt cgggtacttc
    16081 tcatatgacg cggtgcgcta cctggagcgg ctgccggacg ccccgccgga cgagctggac
    16141 gtgccggagg cttacttcgc ggtcacggac acgctcgtcg tcttcgacca tttgcggcac
    16201 aaggtgcttg tgatctcgct ggttgacgcc agcctgttgc gtgacgtgga aggagaaggt
    16261 ttctccgccg cttaccgccg ggccgccgat gacgtgcgcc gggtcgccga gcggctctcg
    16321 gcccctctgg tgcgccgggc gctgccggac ggagccggag gcagcttcga ggtcacgtcc
    16381 aacttcaccg aggcggggta cgtcgaggcg gtcgagaagg cgaaggagta catccgggcg
    16441 ggggatgcgt tccagatagt gccgtcgcag cggttccagg ccgggatcgg agacctcgac
    16501 cccctgctcc tctaccgcgg cctccgaacc gtgaaccctt caccctatat gacgtatctg
    16561 aagctcggcg acctggctat cgtgggcgct tcgccggagc ctctggtgcg ggtcgagggc
    16621 cggcgggtga tgactcgtcc cgtggccggg acccgtccgc gcggagggac gccggaggag
    16681 gacgcggcgc tcgcggagga gcttctcgcg gacgagaagg agcgggccga gcacgtcatg
    16741 ctcgttgacc tcggacggaa cgacctgggg cgggtgagcg agatagggtc ggtggagctg
    16801 gagagcttca tggagataga gcgctactcg cacgtcatgc acatcgtctc gaccgtcgag
    16861 gggaacctgc gcgaggacct caccgccctc gacgccctgg ccgccgcgtt tccggcgggg
    16921 acggtatcgg gcgcgccgaa ggtaagggcc atggagatca tagacgagct ggagcccacc
    16981 cgccgcggcc cctacgccgg cgcgaccggc tactacggca tagacggccg cctcgacacc
    17041 tgcataacgc tccgcaccgc gctgctaaag aatgagacgg cctacttcca ggcaggaggc
    17101 ggcgttgtgg cggactcggt gccgaagctg gagtacgagg agagccgcaa caaggcaagg
    17161 gcgatggcgc gagcgctgga ggtagcccgc agccgggaga tgtggcttta gccaaagggc
    17221 atgaggttct atgcctgagc gctccttata gtcccagctt ctcggatatt tcccggcggt
    17281 caaagccgat gatgacttcc ccgtctatga cagtcgtggg ggtagcctga aaattcatcg
    17341 cgatcagttc ctcgatggcg ttgggatcca tctgaatgtc cttctcggta aaaggtacat
    17401 tattttgaga cagaaactct gtctcctggt gacaggcgtg tcaaccgggc tgggtgtaga
    17461 cgattacttc gtgttcggac acgttttctc ctttcagtcg ctgtggctca gagtacatac
    17521 cacggagccc ggcggaacaa tcagaggaac gccccgctgg ggaggtaagg cgtcagcggt
    17581 ccgagctctc cccggacgca gacgggaccc tgcggcgaga gcgagagacc ggcgtcgagc
    17641 gcgacgcgga cggcccagtc ctgagagccc gtgagccagc gcacctcggt ctctccggtg
    17701 cactccgcaa ggcaagccca cagcaggtcg ccggcgactt cgggagctgt tgcagccaca
    17761 atcgcagggt gcccctcgta ctccacggcg tagcctcgcc cggccggacc ctcggcgacg
    17821 aacaggcgtg ctccggcgcg gagcatcagc tccaggtccg ggccgtgggc ggcgccgcgc
    17881 agtttccggt ctacctctgt ggcgagttcg aggtcctgct tctctccgtc gcggacggat
    17941 ggatccgctg gcagggcttc ccggcgaatg gtgccgctcg ccatgagcgt cggcaggagg
    18001 gtgaagcccg ccagggcgta gcggcgcagg gcggctggat gggtggaggc ggcgatcatg
    18061 gccccctcgc agcccgcagc ataccgtagg gcgtgatcca ggaggtcccg gcctacgccg
    18121 gcgttccggt acgcttcgtc taccgccagg agggagagta tccacacgcc ctcgcgcacg
    18181 agcgccagcg ccgcaccggc cacccggtcc ccatccacgg cgacccacgc gccgccgggg
    18241 tcgtgctcca gcgcgtgccg gtagcgggcc tcgcgccggg cgacctcttc ggcgctccgg
    18301 ttccgcgccc gctccgcctc ttccgcgctc tcggtgaggg cgatgctgcc ggcgcggtag
    18361 gcggcgggaa cgtcatcctg ggtcatgggt cggacacggg ggcgattgct catgcacgta
    18421 tgttagcgtg ccaagagccg aatatttatc gcactctttt tggttacact gcggggatgt
    18481 tcagagagaa gagtgagagg aagtcccggc gccggcatct gggggtatgg gacccaacgg
    18541 gcggcgaggc gcgcgagggt agcgggttgc cggtcgagga gggggtggag aagatccgta
    18601 tcctcgcgcg ggatgtggat ggcggggtgc gggaggtccg ggcggaggag ttggagggcg
    18661 cgatggccgg tgccgcggtc tggatagacc tcgtccggcc gcgggagagg gcggagcgga
    18721 tactgcgcga cgccctgaag ctgccgccgc tcacggtgga ggactgtctg ggaccgctgc
    18781 ggatgccgaa gatggacgtg ttcacggtgg acggggcgcg gggcggcttc gtagcgggtt
    18841 tcgcggcccg cttcgagcgt gccgaaatgg aggtcggact gcgggcggtg gaggtggatc
    18901 tcgtcgccgg tccgggctac ctcgttacgg tgcgggatgg gccggtgccg gaggtcgggg
    18961 agcggctgga gcggaggatg gcggacaagg accttactgc gcaacccggc ttcgcgctgg
    19021 cctacgaggc gatggacgcg ctcatcgacg ggcacctgcc ggcgctggtg gaggcggcga
    19081 cggcggcaga ggagttggac gaagcgctgg acccgagcga cgagcgcgcc tcggtcgcgg
    19141 cgctggaagc tctgatctcg cttcgaagag accttcaagc cttccgccgg ctcgctactg
    19201 cccagcagga gatcctccgc cgcctgggac gtgccgcccc cgacctgcgc gagtacctat
    19261 cagacgcggc ggacaaccag cgcgaggccg tggacatggc cgaggcgacc cgcgactacg
    19321 ccgagggggc ggtcgaggcc taccgcatcc ggcgcgacga gcggagcgag ctcggtatcc
    19381 gccgtctcac ggtcttcgcg gcgatactcg gcccgctcac cctcgtctcc ggtatctacg
    19441 gcgccaactt cgcccgtatt ccggggacgg agagcccgct cggattcccc atttttgtca
    19501 tcgggcaggc actattcgtg gtcgccgccg tatggttcct ctaccggcgc gggctgctgt
    19561 aagaagagca tcccgttaag ctatactctc gcttatggtg acggtattcc tcgtattact
    19621 cggctatgtt ctgggctcgg tgccgaccgg ctatctggtc gggcgcgcct tcggcgtgga
    19681 cgtgcggcag gtcggctccg gcaacatcgg tacggcgaac gtcctgcggg cggcggggaa
    19741 gtggccagcc gtcttcacgc tcgccgggga catggcgaag gggctcctgc ccgtcgtcgt
    19801 cgcccggatc tccacggata acgtgtgggt acatgccctg gtagctttcg cgg
//
LOCUS       DASSMB010000281         4217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1855385, whole genome shotgun sequence.
ACCESSION   DASSMB010000281 DASSMB010000000
VERSION     DASSMB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1855385"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /locus_tag="VFJ72_15990"
     CDS             <1..1782
                     /locus_tag="VFJ72_15990"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7481006.1"
                     /translation="AGTALFVGAFLVFNALSMTILERTRELGMLRALGSTRAMIARSV
                     IVEAAVLGVFGSLVGVLFGYGMAKGLVYLFGRAFLFQITQLVLSPFALVSALVVGVAV
                     TVLAALYPALRAGRVSPVEAMRARSGAAGSEKRRGRVVSVLAPLLGILLVGVGAPWIY
                     YLAKNFSANLSGIIFASGIAAVIGAFLGISLIIPVLVKPLAVVFSPVLRLLFGVEGRM
                     AAANATRNRMRTALTASALMVGISLVVAFSALGGSVLGSIRSYLEDSLGSDYVVQPAA
                     QNSDVTFSSKLPEKIEKVTGVVATTSLASTFRRSGKETSIVFGVDREYPRIFRINYKA
                     GSGTPADAFSKLQSGGAIVGKQLAKSRKLKVAEEISLPGPKGKEKYPVVGIIDNDILG
                     GGQGIYLSKKVLARDFDEKESEFLAIKAAPGSDREAIASGIRKVLKDYPQFGVYSNAE
                     WKARIESDFNRQYVFFYAIMGVSVAVSAFGVVNTLSMSVFERTREIGILRAVGTTRFQ
                     IGRLIVDEGIVISLIGCLVGVAVGSALGYLFVKGSGAGGFEVAFFYPKVPALAALSSG
                     LFIGVFAGLLPARSAARKDIVEAVQYE"
     gene            1908..2408
                     /locus_tag="VFJ72_15995"
     CDS             1908..2408
                     /locus_tag="VFJ72_15995"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET7481007.1"
                     /translation="MAGFPPISVRQPRPHDLVDDPVGVCGIGTGFEGTISARVRAANG
                     NELARRSIHAGGSGIWGNFRVGIDLPGRPPTPRGTLEVFEESESGSGQEPHKVVVPIV
                     FGTNLITGYVGFAQYEVQSGDTLSSIAQQFYGDTDKWRIIFQANRNQIEDPDVIFPGQ
                     VLRIPQ"
     gene            2546..4006
                     /locus_tag="VFJ72_16000"
     CDS             2546..4006
                     /locus_tag="VFJ72_16000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481008.1"
                     /translation="MERCDLRAVERPGTTALPIWLLAAALPAVVGGLWLWWDARSGVP
                     GLLVGALPGALLLSTGLSTLLWARDKRIFQFMSLGAASGMTLSFPAALVFGPVAALVL
                     LAGSAAGFVAAGYLSVWQERVPAEVPEPEMTPGIAARAAQDELSMCGIVLTTWPLSVG
                     ERATQVGRELDEALVLFEERGWKEYSASYHRTPPPLEKPGRGDLRYDHDGFEHLSFES
                     GYEPWPEEPGRERWLSYENNRTAHAWVLRHPGEPRPWMVCIHGIRMGSPRADIRLFQP
                     DYLYRDLGLNLLFPVLPIHGPRRVGPVSGDRILAGDVMDTLHAGSQAIWDIRRLILWL
                     EKGEGAPAVGVLGHSLGGYVAALLAGLAPGLDCVISGNPAVDPSRLFWNNALSLATRY
                     LKAAGVDEARMHRLTRAVSPLEFAPLVNRERLAIFAGVADRVVPPIEAHSLWKHWGHP
                     RIAWYNGTHRGFLRVPEGRDVLEDTLRAAGMLPPNS"
     gene            complement(4003..>4217)
                     /locus_tag="VFJ72_16005"
     CDS             complement(4003..>4217)
                     /locus_tag="VFJ72_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481009.1"
                     /translation="DADGEPLSEEDLIARAWSLSNGEKLDFEAALRTLMLRELVDVKS
                     GGCRVAVGSDEILAYYANLAPSSARR"
BASE COUNT          644 a         1350 c         1460 g          763 t
ORIGIN      
        1 gccgggaccg cgctcttcgt cggggcgttc ctggtcttca acgcgctctc gatgaccatc
       61 ctggagcgga cgcgggagct ggggatgctc cgggctctgg gttcgacccg cgccatgatc
      121 gcgcgctccg ttatagtcga ggcggcggtc ctgggagtgt ttgggtcgct ggtcggcgtg
      181 ctgttcgggt acgggatggc caagggtctc gtgtacctct tcgggcgggc tttcctgttc
      241 cagatcacac agctcgtcct ctcccccttc gccctggtct ccgcgctggt cgtcggcgtc
      301 gcggtaacgg ttctggcggc gctgtatccg gcgctgcggg ccgggcgggt cagcccggta
      361 gaggcgatgc gcgcccgctc cggcgcagcg ggttcggaga agcggcgcgg gcgggtcgtt
      421 tcggtcctcg ctccgctgct cgggatcttg ctggtggggg tcggggcgcc ctggatctac
      481 tacctcgcca agaacttctc ggcgaacctc tccggcatta tcttcgcctc cgggatagcg
      541 gcggttatcg gagctttcct gggcatctcc ctgatcatcc cggtgctcgt gaagccgctg
      601 gcggtcgtgt tctcacccgt cttgcgcctg ctgttcgggg tggagggccg gatggcggcg
      661 gcgaacgcga cgcgcaaccg gatgcggacg gcgctcacgg cctcggcgct gatggtcggc
      721 atctcgctcg tcgtggcgtt cagcgctctg ggcggcagcg tgctgggctc catccgctcc
      781 tacctggagg actcgctcgg cagcgactac gtcgtgcaac ccgccgccca gaactccgac
      841 gtcaccttct cctcgaagct gccggagaag atagagaagg ttacgggcgt cgtggcgacg
      901 acgagcctcg cctccacgtt ccggcgaagc ggcaaggaga ctagcatcgt cttcggggtg
      961 gaccgggagt acccgcgcat attccggatc aactacaagg ccgggtcggg caccccggcg
     1021 gacgccttct cgaagctgca aagcggcggc gccatcgtcg ggaagcagct cgcgaagtcc
     1081 cgcaagctga aggtcgcgga ggagatctcg ctgcccggcc cgaagggcaa ggagaagtac
     1141 ccggtggtcg ggatcataga caacgacata ctgggcggcg ggcaggggat ctacctgtct
     1201 aaaaaggttc tcgcccgcga cttcgacgag aaggagagcg agttcctcgc cataaaggcc
     1261 gcgcccggct ccgaccgcga ggctatagca agcggcatac gaaaggtcct caaggactac
     1321 ccgcagttcg gcgtctactc gaacgcggag tggaaggcgc ggatagagag cgacttcaac
     1381 cgccagtacg tgtttttcta cgcgatcatg ggcgtatcgg tggcggtatc ggcgttcggg
     1441 gtggtgaaca cgctctcgat gagcgtcttc gagcggacgc gcgagatcgg gattctcagg
     1501 gccgtcggga cgacgcggtt ccagattgga cgcctgatcg tggacgaggg gatcgtcatc
     1561 agcctgatcg ggtgcctcgt cggcgtcgcg gtcggctcgg cgctcggcta cctgttcgtg
     1621 aagggctctg gcgccggcgg cttcgaggtc gctttcttct acccgaaagt cccggccctc
     1681 gcggccctct cctccgggct gtttatcggg gtgttcgcgg gactgctccc tgcgagatcc
     1741 gcggcgagga aggacatcgt ggaggcggtg cagtacgagt agccagtgcg ggtagctttg
     1801 cgcctggtcc ctggtgttcg gcgggagtaa gatgtcgttg gaagggacag cgtggcgttc
     1861 gggcatacgg gcacgtcctg tgtccgggcg gcaaggaggt agggatcatg gcaggtttcc
     1921 cgcccatcag cgtccggcaa ccccggccgc acgacctggt agacgatccg gtcggagtct
     1981 gcggcatcgg tacgggtttc gagggcacca tctcggcgcg cgtccgggca gccaacggca
     2041 acgagctggc gcggaggtcc atccacgcag gaggctcggg gatctggggc aacttccggg
     2101 ttgggatcga ccttccgggc cgcccgccga cgccgcgcgg cacgctggag gtattcgagg
     2161 agtcggagtc cgggagcggc caggaaccgc acaaggtagt cgtaccgata gtcttcggca
     2221 cgaacctcat caccggctac gtcggcttcg cgcagtacga ggttcagagc ggggacacgc
     2281 tatcgagcat cgcccagcag ttctacggcg acacggacaa gtggcgcatc atcttccagg
     2341 cgaaccggaa ccagatcgaa gaccccgacg tcatctttcc gggccaggtc ctgcgtatac
     2401 cgcagtagac ggcacttcag acgctgtagc gggtcggtaa gctcgggttc cttctccgga
     2461 gcgttcaacg ttaccggaaa cggctgtcgt cattccgtcg cacggcaggt tccgggatgg
     2521 tacgtttacc ggatcgtaga gacccgtgga gaggtgtgat ctgagagcag tcgagaggcc
     2581 ggggaccacg gcgctgccaa tatggcttct ggcggcggcg ctgccggcgg tggtcggagg
     2641 actgtggctg tggtgggatg cccgaagcgg cgttccgggt ctgctcgtgg gcgctcttcc
     2701 gggagccctt cttctctcca cgggactatc gaccctgctc tgggcccgtg acaagcggat
     2761 cttccagttc atgtcgctcg gagcggcatc cgggatgacg ctctccttcc ccgccgcgct
     2821 cgtcttcggc ccggttgcgg cgctcgtgct gctcgccggg tcggcggcgg gcttcgtggc
     2881 ggcgggctat ctctccgtct ggcaagagcg agtgccggcg gaggtgcccg agccggagat
     2941 gacccccgga atcgcggcgc gggcggccca ggacgagcta tccatgtgcg ggatcgtgct
     3001 gaccacctgg ccgctctcgg tcggggaacg agccacccag gtcgggcggg agttggatga
     3061 ggcgctggtg ctctttgagg agcggggctg gaaggagtat tccgcgagct accaccggac
     3121 gcccccgccg ctggagaaac ccggacgcgg agacctccgc tacgatcacg acgggttcga
     3181 gcacctctca ttcgagagtg gatacgagcc gtggccggag gagccgggcc gcgagcgctg
     3241 gctctcatac gagaacaacc gcacggcgca cgcctgggtg ctccggcatc ccggagagcc
     3301 gcgcccgtgg atggtgtgta tccacggcat aaggatgggt tcgccgaggg cggacatccg
     3361 cctcttccag ccggactatc tctaccggga tctcggtctc aacctgctct tcccggtgct
     3421 accgatccac ggtcctcgca gggtcgggcc ggtcagcggc gaccgcatcc tggccgggga
     3481 cgttatggac accctacatg ccggctccca ggcgatatgg gacatccggc ggctgatcct
     3541 ctggctcgag aagggcgagg gcgctcccgc ggtcggagtg ctcggccact ccctcggcgg
     3601 ctacgtggcg gcgctgctcg cgggactcgc gcccggactc gactgcgtga tctccggcaa
     3661 cccggccgtg gacccgtcgc gcctgttctg gaacaatgcc ctctccctgg ctacccgtta
     3721 cctgaaggcc gccggcgtgg acgaggcgcg gatgcaccgg ctgacgcgcg ccgtctcgcc
     3781 gctggagttc gcccccctgg tgaaccggga acgcctcgcc atcttcgcgg gcgtcgccga
     3841 ccgggtcgtg ccccctatcg aggcgcacag cctctggaaa cactggggcc atccgcgcat
     3901 cgcctggtac aacggcaccc accgaggctt cctccgcgta ccggaaggta gggatgtcct
     3961 cgaagacacc ctccgcgccg ccggaatgct tccacctaac agctaacgcc gcgcagaaga
     4021 gggcgctaag ttcgcatagt aggctaggat ctcatcgctg ccgaccgcga cgcggcatcc
     4081 gccacttttc acgtccacca gttcccgtag cattagcgtc cgcaaggcag cctcgaagtc
     4141 cagcttctca ccgttcgaga ggctccaagc tcgtgcgatg agatcctcct ctgatagagg
     4201 ttccccgtcc gcgtcta
//
LOCUS       DASSMB010000282         6518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1734593, whole genome shotgun sequence.
ACCESSION   DASSMB010000282 DASSMB010000000
VERSION     DASSMB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734593"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VFJ72_16010"
     CDS             complement(<1..881)
                     /locus_tag="VFJ72_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481010.1"
                     /translation="MRSQHATAAPVIRPESAAPDLSGRRLALIRAGWAVLVSLNLILF
                     VVAVPALFVQRSAPPEPVRAGLEQLGLSEGLYAAYFTGLMTIFGLGCFAVAAMIAWRR
                     STDFMGLLASLYLVLAGAVNAPNVQALEAMHPAWELSVKLAQGTFLVSLLLFPFLFPN
                     GRFVPRWTRFLVGFLGLGAVVALFFGGGSASDPPDSLGMLLIAGLLTGAAAQIYRYFR
                     VSDPTQRQQTKWVVSGITVSIVVQVAGTLAPISLSRPGVPALLYGMADVTVVTLAGFL
                     VPLTIGIAILRYRLWDI"
     gene            complement(966..1646)
                     /locus_tag="VFJ72_16015"
     CDS             complement(966..1646)
                     /locus_tag="VFJ72_16015"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HET7481011.1"
                     /translation="MSTVTRTRVAAALGLFSIILSAVGLSIHGYPAIGASGEEIARWA
                     ASTNQQQFGVGVYIEALGTLLFLPFAAWLWSVARDAEGGLGWLATTGFGAVVLFSGIS
                     VMDNGIWSSLLDGARHGTSPQALASIRDIAEYIFHATLLFGGMFFVLTGYVLFRTRVL
                     PRWVGASAVVIGLGLLVPPLAIFAALLVWVWTVVVSLYLLVRPRAVAAVREPSGAMAV
                     PAAAGTRS"
     gene            complement(1738..2400)
                     /locus_tag="VFJ72_16020"
     CDS             complement(1738..2400)
                     /locus_tag="VFJ72_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481012.1"
                     /translation="MSGLALLIALPLQALGFVLHPPSEQVADVLRPLYGPSHLILFVS
                     WIFALPGLAGLYARQANRAGILGLLGFAATVYAAAYHFYLLLYEAYATPLLAHNEATH
                     ALIGPGGPLAHGAGALGPLGGILSLLAFPLFGIAILRAGVLPRLSGWLQIASVLAFFL
                     GPIIVSPDAPAPFGTMAASPIAWMYFLVFLGYAVGGYALWTGKERAHRSAVRSGASQP
                     VA"
     gene            complement(2616..2993)
                     /locus_tag="VFJ72_16025"
     CDS             complement(2616..2993)
                     /locus_tag="VFJ72_16025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481013.1"
                     /translation="MQGSWDGFDVFRERQRELLRETEGRRFVRDALPRRNWRERLSHI
                     SGFLVGIVSKSAHDRRPDEGEDFRVTDSGVPDAVYVIREASGAPCFVVEVFRGRTGRV
                     LRRTDLETGASSYSFLAGRTDGR"
     gene            complement(3130..3954)
                     /locus_tag="VFJ72_16030"
     CDS             complement(3130..3954)
                     /locus_tag="VFJ72_16030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7481014.1"
                     /translation="MGDGRDHKENARLVHDAWERKADFWDERMGDGNLFQRVLVGPAT
                     ERLLGIKPGERVLDVACGNGVFSRRLAALGARVVATDFSEKFIELARARTERAGFDER
                     VEYVMADATDEGRMLALGEKSFDAVVCNMALMDMPTIEPLLRAVRGLLRSDGRFVFSV
                     QHPAFNSNAIRLSLEEEDRDGALIETYSVKVSGYLNVPPGPGAGMPGEPVPHPYFHRP
                     LSELFGACFSAGFVIDGLEEPSFPPQESGQRTARPLSWENFRQIPPVLVARAIPRP"
     gene            4126..5046
                     /locus_tag="VFJ72_16035"
     CDS             4126..5046
                     /locus_tag="VFJ72_16035"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET7481015.1"
                     /translation="MIISAAITGGMTVPGQSAAIPVTPDEIVESAVEAHAAGATIVHI
                     HVREPYTGKPSSDLELFREVVSGIKERCDVIVQPTTGGGKGMTLEERAAVLPGLQPEM
                     ATFNTGSFNFGLFPIAERGGGDRFEEWEVDYLEGTRDYIFRNTFADMEKVCGMFREAG
                     AKPELEAYDVGHLYNIKYLLDKGLLDTPIHLQFVLGVLGANAATIEQFMHMRRTATDL
                     FGSGYTFSAAGVGYPAEYHLAAMCLMLGGHVRVGLEDNLRVRRDKRTDSNAELVEKAV
                     ALAAMFDREPATPDEAREAFGLKGRAAVAF"
     gene            5058..5723
                     /locus_tag="VFJ72_16040"
     CDS             5058..5723
                     /locus_tag="VFJ72_16040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET7481016.1"
                     /translation="MSRVCVFDVNETLLDLAALDPHFERAFGDASVRQQWFMQMLQST
                     FVATITDAYATFGEIGVAALQMTAERQGITISDEDRQSILGGMRELPPHPEVSEALDR
                     LRDAGIRLAALTNSTPEVAEAQLNHAGLAEKFERILSADTAKRLKPAAAPYRMAARSL
                     GIDIRELRLVAAHAWDVAGAMRAGCAAAFVARQPFDPLVEKPDVVGADLAEVADGIIA
                     AET"
     gene            complement(5739..>6518)
                     /locus_tag="VFJ72_16045"
     CDS             complement(5739..>6518)
                     /locus_tag="VFJ72_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7481017.1"
                     /translation="GDFNLCQKAYAFWSQPPEVEPHFHGAFATHYYVHPEQYFYKVPD
                     NVPDEVVAGANCGLTQVLFGLHQIGLTAGDSLVIQGAGGLGLYAAAVGRDMGAQVIMV
                     EGISERIELARRFGAEEAVDMREHETAEERVERVQDLTGGYGADVVLEVTGVPAAFVE
                     GVQLARPGGKMVEIGNVSVGKEHEVSLAPGLITRKMLRVQGFVRYQPWFLHRALRFLE
                     RKHKDHPFDELSDREYSLEDVGEAITREEAKKVARPAIVPA"
BASE COUNT         1217 a         2253 c         2067 g          981 t
ORIGIN      
        1 tctatgtccc acagccggta gcgtaggatg gctatgccga tggtgagcgg gacaaggaac
       61 ccggcgaggg taactacagt cacatcggcc atgccataga gcagcgctgg gacgccgggg
      121 cgggaaagtg agatgggcgc cagggtaccc gcgacctgca ccactatgga cactgtaata
      181 ccagagacga cccacttggt ctgctggcgc tgcgtcgggt ccgagacgcg aaagtaacgg
      241 tagatctggg ccgccgctcc ggtgaggaga ccggcaatga gcaacatacc gagggagtcg
      301 gggggatcgc tcgccgagcc tccgccgaag aacagcgcga cgacggcccc caggcctaag
      361 aaaccaacca aaaaccgcgt ccagcgaggc acgaacctcc cgttggggaa aaggaaagga
      421 aagaggagca gcgagaccag gaaagtcccc tgcgcgagct tgaccgacaa ctcccaggca
      481 ggatgcatgg cctccagcgc ctgcacgttg ggagcgttga ccgcgccggc caacactaga
      541 tacaacgatg ctaacaaccc catgaagtcg gtggagcggc gccaggcgat catggcggcc
      601 acggcgaagc accccagccc gaagatggtc ataagtccgg taaagtacgc ggcgtagaga
      661 ccttcggaga ggccaagctg ctccagtccc gcccgcacgg gctcgggtgg ggcgctgcgt
      721 tggacgaaca gcgcgggcac ggcgacgacg aagaggatca ggttcaggga gaccagcacg
      781 gcccacccgg cccggatgag agcgagccgg cggccgctca ggtccggcgc cgcactctcc
      841 gggcgtatca ccggcgctgc tgtggcatgt tgcgaacgca tcaagataat tctactcccc
      901 taccgcagcc ctcgacgagt tgtgctcacg ccgtactgcg ggttcgccct ccggttcctc
      961 gccggtcaag agcgcgtacc tgccgcggcc ggaaccgcca tggcgccgga tggctcgcgc
     1021 accgccgcga cggcgcgggg acgcaccagg aggtacaggc tcaccaccac ggtccaaacc
     1081 cagacgagta gggccgcgaa gatggcgagg ggtgggacaa gcaaccccag accgatcacg
     1141 accgcgctgg cccccaccca acgcgggagg acgcgggtgc ggaacaagac gtagcccgtg
     1201 agaacaaaga acatgccgcc aaacagaagc gtggcgtgga agatgtattc cgcgatgtct
     1261 cgaatgctgg cgagcgcctg cggactcgtg ccgtggcgag cgccatcgag aagtgaagac
     1321 cagatgccat tatccatgac gctgataccg gagaagagca cgacggcgcc gaagccggtg
     1381 gtggcgagcc aacccaggcc cccctctgcg tcgcgggcca cactccagag ccaggcggcg
     1441 aacgggagga agagcagggt tccaagtgcc tcgatgtaga ccccgacccc gaactgctgc
     1501 tggttcgtcg aggccgccca gcgcgcgatc tcctcgccgc tcgcgcctat ggccgggtag
     1561 ccgtggatag acagcccgac ggcagacagg atgatgctga acaggccgag agcggcggct
     1621 acacgggtac gcgttacggt cgacatttct ctctcctttc cttgcccggt agttatccgg
     1681 cgcttccatt ggcttctatt gatttgaagg gcgaggggtt gctcccctgt tgaggcccta
     1741 cgcaacgggc tgggatgcgc ctgatcggac cgcggatctg tgtgcccgct ccttccccgt
     1801 ccagagcgcg tatccgccga cggcgtatcc caggaaaacc aggaagtaca tccaggcgat
     1861 gggcgacgcc gccatcgtcc cgaacggagc gggagcgtcg ggcgacacga tgatcggacc
     1921 gaggaagaaa gccaggacgc tggcgatctg caaccacccc gaaaggcgcg gcaggacacc
     1981 ggcgcgcagg atagctatcc cgaagagcgg gaaggccagg agcgaaagga tgccgccgag
     2041 aggaccgagc gccccggccc cgtgcgccag cggtccgccg ggaccgatca gtgcatgagt
     2101 ggcctcgttg tgggcgagca gcggggttgc gtacgcctca tacagcaaga ggtagaaatg
     2161 atacgcggcg gcgtacacgg tggcggcgaa gccgagcagg ccaagaatac ccgcccggtt
     2221 cgcctggcgc gcgtagagtc ccgctaggcc aggcagcgcg aaaatccacg atacgaacag
     2281 gatcaggtgc gatggtccat aaagtggcct gagcacgtct gccacctgct cgctcggcgg
     2341 gtgcaggacg aaacccaggg cctggagcgg cagggcgatc agtagagcca gaccgctcaa
     2401 ccggaacaac attcgcgacg acatcgctta cctccttcgc gggacctccc gcacttgctg
     2461 gagcaggccc catgcctgcg tgtatgagag cactatgggc gcgggacttt cccgggaggt
     2521 agaggtcgtt ttcccgaacg gaccgggcaa tttttcacat gaggttctcg gactctggag
     2581 cttgctggac cggcgggaga gcttccccgt cgcgtttacc gcccgtccgt ccggcccgcg
     2641 aggaaagagt aggagcttgc gccggtctcc aggtccgtcc gccgcagtac gcgcccggtg
     2701 cggccccgga agacctctac cacgaagcat ggtgcgccgg aggcttcccg gatcacgtag
     2761 acggcatccg ggacgccact gtccgtcacc cggaagtcct ccccttcgtc cggcctccgg
     2821 tcatgcgccg acttactcac aatcccgacc aggaaccccg atatatggct caggcgctcg
     2881 cgccagttcc gacgcggcaa ggcgtcgcgc acaaaccgcc ggccctccgt ctccctcaac
     2941 aactcccgct ggcgctcccg gaatacgtcg aaaccgtccc aagaaccctg catcttcgct
     3001 cccctcgtcg tcgccgcggc gaccgtggat tccgccagat ctcaacctcg attacgcgct
     3061 ctaaagatac tggagataga ggcttctgac atcgccgtat ttgccgtacc tatgcggaaa
     3121 atcgtggatt caaggtctcg ggatcgcccg cgcaacgagg accggcggta tctgccggaa
     3181 gttctcccag gagagcggtc tcgcggttct ctggccgctc tcctgcggag gaaacgacgg
     3241 ctcttcgagg ccgtcgatca cgaaccccgc agagaagcac gccccgaaca gttcgctaag
     3301 gggacgatgg aagtacgggt gcggaacggg ctctccgggc atgcccgctc ccggtcccgg
     3361 cggtacgttc aggtagccgc tcaccttgac cgagtaggtc tcgatcaggg ccccgtcccg
     3421 gtcctcttcc tccagagaca gccggatggc gttggagttg aaggcgggat gctgcaccga
     3481 gaacacgaac cgtccgtcgg acctgagcag acccctgacc gcccggagca gcggctcgat
     3541 ggtcggcatg tccatcagcg ccatgttaca gaccaccgca tcaaagctct tctctccgag
     3601 agcgagcatc cggccctcgt cggtggcgtc cgccatcacg tactcgaccc gttcgtcgaa
     3661 ccccgcccgc tcggtacggg ctctcgccag ctcgatgaac ttctcgctga agtccgtggc
     3721 caccacccgc gcgcccagag cggcgagcct gcggctgaac accccgttgc cgcaggccac
     3781 gtcgagcacc cgctcgccgg gctttatccc gagcagcctc tcggtggcgg ggccgacgag
     3841 cacgcgctgg aacaggttcc catcgcccat cctctcgtcc cagaaatccg ccttcctctc
     3901 ccaggcgtcg tggacgagac gcgcgttctc cttgtgatct ctaccatcgc ccatgccggc
     3961 tctcctcccg tatacgcacc cggatcggtc ccggtccggg gcatcacgat acaggcgaga
     4021 ccggaaaggg ggaagtgcgg ggatctacat acgcgattgc ggcggcccgg atgggcctct
     4081 agtattgccg gagggacgcg agggaaggag cgggtcttgg agaaggtgat catcagcgcg
     4141 gcgatcacgg gcggcatgac ggtgccgggc cagagcgcgg ccataccggt aacgccggac
     4201 gagatcgtag agtccgcggt cgaggcccac gcggcgggcg cgaccatcgt ccacatccac
     4261 gtccgcgagc cgtacaccgg caagccaagc tccgacctgg agctcttccg cgaggtcgtc
     4321 tccggcatca aggagcggtg cgacgtgatc gtacagccca ccaccggcgg cggtaagggc
     4381 atgaccctcg aagagcgcgc cgccgtcctg ccggggctcc agcccgagat ggcgaccttc
     4441 aacaccggca gcttcaactt cggcctcttc cccatcgccg agcgcggcgg cggggaccgc
     4501 ttcgaggagt gggaggtaga ctacctggag gggacccgcg actacatctt ccgcaacacc
     4561 ttcgcggaca tggagaaggt ctgcggcatg ttccgcgagg caggcgcgaa gccggagctg
     4621 gaagcctacg acgtgggcca cctctacaac ataaagtacc tgctggacaa ggggctcctc
     4681 gacactccga tccacctgca gttcgtcctc ggcgtgctgg gcgcgaacgc cgccaccatc
     4741 gagcagttca tgcacatgcg ccgcaccgcg accgacctct tcggctccgg ctacaccttc
     4801 tccgccgccg gcgtcggata ccctgccgag taccatctgg ccgcgatgtg cctgatgctc
     4861 ggcgggcacg tccgggtcgg cctggaagac aacctccgcg tccgccgcga caagcgcacc
     4921 gactcgaacg ccgagctggt cgagaaagcc gtcgccctcg ccgccatgtt cgaccgcgag
     4981 ccagcaaccc ccgacgaagc ccgcgaggcg ttcggcctca agggccgcgc cgccgtagcg
     5041 ttctagagag gaacttcatg agtagagtct gcgtcttcga cgttaacgag accctgctgg
     5101 accttgccgc cttggatccc cacttcgagc gggccttcgg cgacgcctcc gtgcgtcagc
     5161 aatggttcat gcagatgctc cagtccacgt tcgtcgccac gatcacggac gcctacgcga
     5221 ccttcgggga gatcggggtc gccgccctcc agatgaccgc cgagcgccag ggcataacga
     5281 tctccgacga ggatcggcag agcatcctgg gcggtatgcg ggagcttccg ccccacccgg
     5341 aggtctccga agccctcgac cgtctgcgcg acgccggtat ccgcctcgcc gcgctcacca
     5401 actcgactcc ggaagtcgcc gaggcgcagc tcaaccacgc cggtctcgcg gagaagttcg
     5461 agcggatcct ctccgccgac accgcgaaac gcctcaagcc ggccgccgcc ccctaccgaa
     5521 tggccgccag gagcctcggt atagatattc gcgagctgcg cctcgtcgcg gcccacgcct
     5581 gggacgtggc gggagccatg cgcgccgggt gcgccgccgc cttcgtcgcc cgccagcctt
     5641 tcgatcccct tgtggagaag ccggacgtcg tcggcgcgga cctcgccgag gtcgccgacg
     5701 gcatcatcgc cgccgagacg taaggctgcg cgcgtagctt atgccggcac tattgccggc
     5761 cgcgccacct tcttcgcctc ctcccgagta atagcctctc cgacgtcctc cagggaatac
     5821 tcccggtccg agagttcgtc gaacggatgg tccttatgct tgcgctccag aaagcgcagc
     5881 gcccggtgga ggaaccatgg ctggtagcgc acgaagccct gcacgcgcag catcttgcgc
     5941 gtgatcagtc cgggcgccag cgagacctcg tgctccttgc cgacgctgac gttcccgatc
     6001 tcgaccatct tcccgccggg ccgcgcgagc tgcacgccct cgacgaacgc cgcgggaacg
     6061 cccgtgacct ccaggaccac gtccgcgccg tagccgccgg tgaggtcctg cacccgctcc
     6121 acccgctcct cggccgtctc gtgctcccgc atgtccaccg cctcctcggc accgaagcgc
     6181 cgggcaagct cgatccgctc cgagataccc tccaccatga tgacctgcgc acccatgtcc
     6241 cggccaacag ccgccgcgta cagacccagg ccgccggcgc cctggatcac caggctgtct
     6301 ccggcagtga gcccgatctg gtgcagcccg aagagcacct gcgtgagccc gcagttcgcc
     6361 ccggccacca cctcgtccgg gacgttatcc ggcaccttgt agaagtactg ctccgggtgg
     6421 acgtagtagt gcgtggcgaa cgccccgtgg aagtgcggct ccacctccgg cggctgcgac
     6481 cagaacgcgt aagccttctg gcacaggttg aagtcccc
//
LOCUS       DASSMB010000283         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_493183, whole genome shotgun sequence.
ACCESSION   DASSMB010000283 DASSMB010000000
VERSION     DASSMB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493183"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFJ72_16050"
     CDS             complement(<1..644)
                     /locus_tag="VFJ72_16050"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7481018.1"
                     /translation="MNLREMMETRVLIGDGAMGTLLAERGVGFGHPYARANLTHPEMV
                     AGIHEEYLRAGATVIETNSFSANRYKLAAHELEDKVREVNLEGARLARKAADTVAGSG
                     EKALVLGAIGPLGRPLAPIGPVSPEEARTVFLEQAEALLEGEADALLLETFTDLAELK
                     LAYDAVRGLGVPVLAYKTFVEDGETLAEGLPERAAREISSWGADLTGANCTVGP"
     regulatory      complement(722..844)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(905..1777)
                     /locus_tag="VFJ72_16055"
     CDS             complement(905..1777)
                     /locus_tag="VFJ72_16055"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET7481019.1"
                     /translation="MSHSRTYYFSTQLFPKGVRPHVHALYAFMRYADEIVDTPGALSL
                     EEQLAGLERFERETIRAISGEEVLNPVLRAFAYTVRTRGIKLESITAFMKSMKMDTDT
                     FRYRTYGELEAYTYGSAAVVGLMMCRVAGVRDDRATTHAEALGVAMQLTNFLRDMGED
                     WRRGRVYVPLEDLERFGYSEGDLAKGVVDERFAALMEFEIERARRLYAQADEGMRYIP
                     RGRRYPVYVARKLYAAILDRIEAQDYDVFSRRADTSRARKVLVAGAVAARHPGELLPG
                     GGGGKVDPEIAIRR"
     gene            complement(1863..>3059)
                     /gene="crtI"
                     /locus_tag="VFJ72_16060"
     CDS             complement(1863..>3059)
                     /gene="crtI"
                     /locus_tag="VFJ72_16060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HET7481020.1"
                     /translation="TLTIRRALPELIKEIERIEPGVTPNFYRFLEDACYKYRLGRSEF
                     VERDFDKAKDFFGLRNLRLLLKTKAVNNYYKQVSKFFTSDKLRQAFSLQTMYLGLSPF
                     EAPAVYSLLPYTELAEDGLWFPEGGMYALVEAMEKLATELNVRFRLNTEVEKVVVTKG
                     RARGVRVNGEEIEADAVLANADLPYAYRDLLDGAADSDFKLRKRDNLKYTASAFMLYL
                     GIDGKLEQMLHHNFYLSSRYRENFEAIFRDGRLPEDPSFYAVVPSKTEPRMAPEGMDS
                     LFVLVPVPHLGPNVDWGRDGEVFREKVYGLLERRCGVARERVVYEKVKTPLDWRDDYN
                     LEEGAAFGIGHGIFQVGYFRPPMVSRAVEGMYFVGASTRPGTGVPLVTIGARLVAERI
                     EREVGV"
BASE COUNT          492 a         1062 c          926 g          579 t
ORIGIN      
        1 tgcggcccga cggtgcagtt cgccccggtc aggtccgcgc cccaggagga gatctcgcgc
       61 gccgcccgct ccggcaggcc ctccgcgagc gtctccccat cctccacgaa cgtcttgtac
      121 gccagcaccg gcacccctaa ccctcgcacg gcgtcatacg ccagtttcag ctccgccagg
      181 tccgtgaagg tctccagcag cagcgcgtcg gcctcgcctt cgaggagcgc ctcggcctgc
      241 tccagaaaga ccgtgcgcgc ctcctccggg ctaacgggac cgatgggggc gagcggccgt
      301 ccgagcggtc cgatggcgcc gaggaccagg gccttctctc cgcttccggc gacggtgtcg
      361 gcggccttgc gggcgaggcg ggcgccttcg aggtttactt cgcggacctt gtcttcgagc
      421 tcgtgagcgg cgagcttgta gcggttcgcc gagaaggagt tggtctcgat gacggtggcg
      481 ccggcgcgca ggtactcctc gtggatgccg gcgaccatct ccgggtgggt gaggttggcg
      541 cgggcgtagg gatggccgaa gcccacaccg cgctcggcca gcagggtgcc catcgcgccg
      601 tccccgatca gtacccgcgt ctccatcatc tctctaaggt tcaagctcaa agataaacct
      661 ctctgtaagt ttccactcgt gcggaagaga gaggctgcgt cgaatcaagt tctctgtctt
      721 cgactcatct ctcggagtct cctccgcggg agttggcacc tggcacgagg cttacgctct
      781 ggcgttcgtc tcgccggttg ccgcggtttc acagggccca tccctccacc gctctcgatg
      841 agcgccgcga cagccgttgg atcgtcctcg gctggcggct ttcagtcagg caattgaacc
      901 acgatcagcg ccggattgca atttcggggt ctaccttgcc gcctccacca cccggcagta
      961 actcgcccgg atgccgcgcc gcgacagccc ccgcgacgag gactttcctc gcgcgcgacg
     1021 tatccgctct ccgggagaac acgtcgtagt cctgagcttc gatcctgtcg aggatcgccg
     1081 cgtagagttt tcgcgccacg tataccggat accgccgacc gcggggtatg tagcgcatcc
     1141 cctcgtccgc ctgcgcatag agcctccgag cacgctcgat ctcgaactcc atcagcgccg
     1201 cgaaccgctc gtctaccacg cctttcgcca ggtctccctc cgagtatccg aacctctcca
     1261 ggtcttccag gggcacgtag actcgcccgc gccgccagtc ctctcccatg tcccgcagga
     1321 agttggttag ctgcatcgcc acgcccaggg cttcggcgtg ggtcgtggcc cggtcgtccc
     1381 gtaccccggc cacccggcac atcatcaggc cgacgaccgc tgcgctcccg taagtgtagg
     1441 cctccagttc tccgtaggtc cggtagcgga aggtgtccgt gtccatcttc atgctcttca
     1501 tgaacgccgt gatgctctcc agcttgatcc cgcgggtacg gaccgtgtag gcgaaggcgc
     1561 gcaagaccgg gttcaggacc tcctcgccgg agatcgcccg gatggtctcc cgctcgaagc
     1621 gttcgagacc ggcgagctgt tcttcgaggc tcagggcgcc cggagtgtcc acgatctcgt
     1681 cggcgtagcg catgaaggcg tagagcgcgt ggacgtgcgg ccggacccct ttggggaaga
     1741 gttgcgttga aaagtagtat gtcctgctgt gggacatctg gatcctacgg cacaactcgt
     1801 aacagcccgc gagactcaac ctgccatcga gggtctggcc gctcgccggg tccagcctca
     1861 cgttaaaccc cgacctcgcg ctcgatgcgc tcggccacca gccgcgctcc tatggttacc
     1921 agcggcacgc ccgtccccgg ccgcgtgctc gcccccacga agtacatccc ctcaacggcc
     1981 cgcgagacca tcggaggccg gaaatacccg acctggaaga tgccgtgacc gatcccgaac
     2041 gccgccccct cctccaggtt gtagtcgtcc cgccagtcca gcggcgtctt gaccttctcg
     2101 tacaccaccc gctcccgcgc caccccgcaa cgccgctcca ggagcccgta caccttctcc
     2161 cggaacacct ccccatcccg gccccagtcc acgttcggac caagatgcgg aaccggcacc
     2221 agcacgaaca gcgagtccat cccctccggg gccatgcgcg gctccgtctt cgacggcacg
     2281 acggcgtaga aggagggatc ctccggcagc ctcccgtccc ggaagatcgc ctcgaaattt
     2341 tccctgtacc tcgacgagag atagaagttg tgatggagca tttgctccag ttttccgtct
     2401 atcccgaggt agagcatgaa cgccgacgcc gtgtatttga ggttatccct cttcctgagc
     2461 ttgaagtccg agtcggctgc tccgtcgagc agatcccggt aggcgtaggg taggtcggcg
     2521 ttcgccagca ccgcgtccgc ctctatctcc tcgccgttta cccggacgcc gcgcgcccgg
     2581 cccttcgtca cgacgacctt ctcgacctcg gtattcaacc tgaacctgac attcagctct
     2641 gtcgccagct tctccatcgc ctcgacgagc gcgtacatcc cgccctccgg gaaccagagg
     2701 ccgtcctcgg cgagctcggt gtaggggagg agcgagtaga cggcgggcgc ctcgaagggg
     2761 gagaggccca ggtacatggt ctggaggctg aacgcctggc gcagcttgtc cgatgtgaag
     2821 aacttcgaga cctgcttgta gtagttgttg acggcctttg ttttcaggag caggcggagg
     2881 tttcggagtc cgaagaagtc cttggcctta tcgaagtcgc gttcgacgaa ctcggagcgg
     2941 ccgagacggt acttgtagca ggcgtcttcg aggaagcggt agaagttcgg ggtgacgccg
     3001 ggttctatgc gttcgatctc ttttataagc tccgggaggg cgcgccggat cgtgagcgt
//
LOCUS       DASSMB010000284        56479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_620077, whole genome shotgun sequence.
ACCESSION   DASSMB010000284 DASSMB010000000
VERSION     DASSMB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 56479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56479
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620077"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VFJ72_16065"
     CDS             <1..898
                     /locus_tag="VFJ72_16065"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7481021.1"
                     /translation="ESGEVIVLDGATGTELERRGVPMDDAAWCAAALVTHPEVVREVH
                     EDYIRAGADVVTTNTFPTARHVLEPAGMGERFRELNALAVELARQSRENAAGSAGPVY
                     IAGSISTFSARHDNSYEPSEKTARANYREQAEILAEAGVDLIALEMLRDIEQAGYAVE
                     AAVSTGLPIWAGFSCKRAADGTVVLWDGGHTLAEALAEIPPLGVSLVSVMHTLIEDAV
                     PALREVVEGWDGPVGAYPHAGKFIMPNWQFTDMISPEDFAERARGWVDLGARVIGGCC
                     GIGPDHIRAIRESFPSSVAAGR"
     gene            1030..2592
                     /locus_tag="VFJ72_16070"
     CDS             1030..2592
                     /locus_tag="VFJ72_16070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7481022.1"
                     /translation="MAIGLATVTVAWLIVNLLTGWTHFALLVNPKLSGGEEAASALTR
                     FFGALVLVLFFAENEGWRLRWVAAGLVVLGLGHLTFGYLEPTIQNDPAELNESLYEAL
                     VTQILACALFVIGLVPRRSPRLRTWVPLVILACVPLAGYIYIFELLRDETWMPALTVL
                     ESAAETVEAGTPPEWLTVWHWIFSSIPLVLATTAAVGGIMRSRGSGGDVRRAPWWWLT
                     PAIVLLAGAQLHEVFWPSGYAGRVLTTAEGLRLAFSVVVVVGGIIELWRIGSERATLL
                     AAEEERRRRLEELAVMKADFSAMVAHELGSPLAAIRGYADMLAAPELEPDARSSALKA
                     IRGEISALNTLVDDVRDAATGEREDFAVEPRPVSLETLLTEAAAFAATLPGGHELTVV
                     RETGARVVRADAERIGQVLRNLLSNAAKYSPEGAPIQLRAVPGGGGRVRLEVADKGPG
                     IDPGDIGRILEKFGRGKDAAGKKVAGVGLGLYLSRRIVSSHGCELEVRSTSGEGSVFA
                     FELEVIEEGDEQ"
     gene            2589..3257
                     /locus_tag="VFJ72_16075"
     CDS             2589..3257
                     /locus_tag="VFJ72_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7481023.1"
                     /translation="MIRVMLVDDHASFLQPLAFMFDREPDIMVVARAESVAQARERIE
                     ELKAADSNVDLALLDLDLPDGSGTEVISILRDANPEALALILTSFSERDQLAPAIEAG
                     AAGVMHKSVEISEIVDAARRLHAGEQLISPSEVIEAMRFIGRRRQEDYEAQRMAESLT
                     QREREVLQALADGLGDREIADRLHVGIGTVRTHMVNILRKLDVASRLQALLFAVRHDV
                     VTIN"
     gene            3378..3749
                     /locus_tag="VFJ72_16080"
     CDS             3378..3749
                     /locus_tag="VFJ72_16080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481024.1"
                     /translation="MDMTEHTLTPDAGAPERPRVLVANDPGAYRDAISRALRVMRPEV
                     EVLDIAPAELDREYARLRPALTVCSKLTGAVEAGPSDWVELYPDGGSVAHLSVGGERS
                     TLARPDFCIILSLVDRLLYRT"
     gene            3852..5030
                     /locus_tag="VFJ72_16085"
     CDS             3852..5030
                     /locus_tag="VFJ72_16085"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7481025.1"
                     /translation="MKKPERLARTRARKRARDRTPAREAAPTPIFVSPRVLALLVGII
                     VVVFALVIYAAPIIPVVALGGMALAIVLSFPVRALSRLMPRGVAILVTFLGLIGLVTL
                     ALVFLVPLLVRQLGNFILIVPTIANDVNRFLLDLIQPLRERDVFLIPSDQFMQGLVQD
                     LFGRAQEVIQGILGGLVGFISGAFSFGIALFGVLFVAVYLLVDVRKVKAAFLKAAPVH
                     YRHDARELWDAFGFSLSRYLGGLLLVVVVQGVLATLALQILGVPYAILLGVWVSLTAI
                     IPYLGPFLGAIPALIVALTFGSPAFENNTTTAILTMVAYVLIQQFEGNFLTPRVQGHA
                     LHVHPILVLLAVIGGGQLAGLAGVIFAVPTLAILRVFFDFFRARLRTPLARAPQEPEL
                     "
     gene            complement(5037..6413)
                     /locus_tag="VFJ72_16090"
     CDS             complement(5037..6413)
                     /locus_tag="VFJ72_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7481026.1"
                     /translation="MTHSRTNDGGSGRATDGMTRKQFLKVGGAGLAGAALLGSGALAG
                     CGGGGGSGAGGGSGSITFSWIPEQTGTLQKMIDRFNKENEDGIKVKFQEMPTDSTQHF
                     DKLRTQFQAGSTDMDVIGGDVVWPAQFAAPGYYLDLSDRFSESARNKFLPAPMQANTF
                     KGKPYGVPWYTDSGMLYYRKDLLEKSGFSDPPKTWDELEQMAKKVMQDSGTKYGFVFQ
                     GAQYEGGVCNGLEYIWTHGGDVLDPDNPNKVVVDSPGSIAGLAAERSMVDAGVAPGAV
                     SSYKEQEAQTTFLNGDSVFMRNWPYIYGLAADESESKVKQSKIGVAPLPVGKEGDSSF
                     SALGGWNFSINAKSKNPDAAWSFIQYMTAPAQEKEFALKTARIPALKDLLDDQELVSK
                     VPVIKLGKEAISRTRPRPVSAYYSDISLAMAKQFNSSLNGETSPKGAIKTLDSELKDI
                     VKQGKSQE"
     gene            complement(6492..7571)
                     /gene="dapE"
                     /locus_tag="VFJ72_16095"
     CDS             complement(6492..7571)
                     /gene="dapE"
                     /locus_tag="VFJ72_16095"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET7481027.1"
                     /translation="MSGEPLAERLLETLLFFLERPSVIGEEKRLCDDLEARIAKLPGW
                     ETRRVSNNLIVRRAQRDASRPTIVFAGHLDTVPEPAGGIEVRVEGDRVYGRGASDMKA
                     GDAVMLALLEGLEWETCRAEPVFVFYEREEGPYAENGLETVFAEVPWVLDANLALVPE
                     PTAGALEVGCVGTAQVEVTFRGKPAHAARPWQGENALTKAGAFLAALHERAANEVIVE
                     GLPFYEVLTPTMARGGRAKNVVPDEFWINVNHRFAPGRDIEDVKRLFDSLLDGGASYE
                     ITDFAPSGPVSLDNSLLRELVEGGLEVRPKQAWTDVARFAQRGVAAANYGPGLPSQAH
                     QQAEYAGLPLLAECYERLVSFLKHP"
     gene            complement(7568..8374)
                     /locus_tag="VFJ72_16100"
     CDS             complement(7568..8374)
                     /locus_tag="VFJ72_16100"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET7481028.1"
                     /translation="MREKIEAAFEDRGLLKDEEHRSAVFDTIAALDRGELRVAEKRNG
                     DWESNAWVMKAINLYFGVAEMKTIEAGPFEYHDKIPTKKNLAEAGVRVVPPGTVRYGA
                     FVEPGVVVMPGYVNIGAYVGTGTMVDTWATVGSGAQIGRNVHLSGGVGIGGVLEPPGA
                     MPVVIEDGAFIGSRAIVVEGVRVEEEAVLGANVVLTGSTRIIDVTGPEEVVHRGRVPA
                     RSVVVAGTRARDFPAGSYQIQTALIIGQRRESTDQKTSLNDALREFGVSA"
     gene            8552..9415
                     /locus_tag="VFJ72_16105"
     CDS             8552..9415
                     /locus_tag="VFJ72_16105"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7481029.1"
                     /translation="MTSLALALVLAAAVFHATWNLFAKRVGDGGAPFVWLFGALSALI
                     YAPLAAVVVFVERPHFGPMELLFMGGSGVLHLGYFVLLQRGYATGDLSLVYPLARGTG
                     PLLATAAAIALLGERPSALALCGVALIAVGVFLLTREPGSVRGAGFGRAAIYGLLTGG
                     FIAAYTLWDKHAVSALLIPPLLQSWATTVVTTALISPLAFRHRGKVRALWRTHKFEAI
                     GVGVLSPLSYILVLTALVFTPVSYVAPAREISILIGTAMGARLLAEGDTARRLVAASA
                     MVAGVVALALG"
     gene            complement(9503..9943)
                     /locus_tag="VFJ72_16110"
     CDS             complement(9503..9943)
                     /locus_tag="VFJ72_16110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7481030.1"
                     /translation="MAIKRLDHVSVVVDDLAAAIAFFSELGLELEGEAAVEGEWVDRV
                     NRLESVQVDIVMMRTPDGHGRLELTKFHNPKLVEIEPAIAPPNTLGLRSVMFTVESVD
                     DTVARMHAHGAELVGEVAQYENLYRLCYMRGPGGIIVALAEELF"
     gene            complement(10126..10623)
                     /locus_tag="VFJ72_16115"
     CDS             complement(10126..10623)
                     /locus_tag="VFJ72_16115"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7481031.1"
                     /translation="MEIRHAIEADLPVILEIYNSTIPSRMVSADTEPVSVEQRLPWFR
                     AHDPARRPILVAEEGGEVVGWISLSDFYDGRPAYHQTAEIGIYVRDGFRGRGIGRRLL
                     EEVIRRAPELGIKTLTAGAFGHNGPSLKLFERFGFERWARFPQVAELDGAERDLVVLG
                     LRLEA"
     gene            complement(10651..11859)
                     /gene="dapC"
                     /locus_tag="VFJ72_16120"
     CDS             complement(10651..11859)
                     /gene="dapC"
                     /locus_tag="VFJ72_16120"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HET7481032.1"
                     /translation="MSEPGATTSTDHQPLKSGPPPLLLNPVLEWQGAYPLLRLDERRR
                     ELQERGTDLYDFGTGDPREPTDAKIRQALIQGVPEVSQYPSSPGKRELREAFAGWMRR
                     RHGVALDPETEVLPATGSKEAIFHAPLALLHHSHERRGAAYGTPGYPVYERGTQFAGG
                     EPLPVRLRREDGFLLPVESVDPARTRILWINYPHNPTGATATYEYLEGVAEFCREHDI
                     LLFSDECYNDIYSDTPPPSILEVTRERTLAFCSLSKRSGMTGYRSAMMAGDPELIAAL
                     KKMRPSIGVATQAFVQDAATAAWSDDEHVEDRRRIFADKRRLFIDFFERSGLEYLPTD
                     ASFYLWVTVPEAFAGDDEAYALRLMEEGIVVAPGRSFGPGGAGYVRVALVPELADCRR
                     AIELWERVGV"
     gene            11947..13206
                     /gene="lysA"
                     /locus_tag="VFJ72_16125"
     CDS             11947..13206
                     /gene="lysA"
                     /locus_tag="VFJ72_16125"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7481033.1"
                     /translation="MDDFHYEGGDLYCEDVPLAEIARAAGTPTYVYSHAALERAYRDL
                     DDAFSGLDHLVCYAVKANGNLAVLRALASFGAGADIVSGGELYRVMRAGFDPKKVVFA
                     GVGKTEQELMAGLGERILLFNIESASELEHLERLASRHGKKARVSLRVNPNVDAETHE
                     HITTGRERDKFGIPVDEALALAERIGEFRCVDLIGVHQHIGSQITKIDPHTEAIERSA
                     GLVEELKHRGFDIKYFNIGGGLGIRYKDEKVPTAKDLVDAVRPTLESLGASGTKILCE
                     MGRYIAGNAGALLTRVIYRKKSGEKSFIVADAGMNDLIRPSLYDAHHEVRAAREDGSL
                     ANADLVGPVCESGDYLARDRELPDAAEGELLAVMSAGAYGFSMASNYNSRPRPAEVMV
                     RGDSFAVVREREHNADLIKGELVPAFL"
     gene            complement(13291..13785)
                     /locus_tag="VFJ72_16130"
     CDS             complement(13291..13785)
                     /locus_tag="VFJ72_16130"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HET7481034.1"
                     /translation="MRAMAWVRILIGAVWLNGAIEKLLNPDFPQQFAQSLKAGGFVSQ
                     APPFFQGFMKGTVVPNAELFAQVARVVELSLGIALILGLLTNLAAIGSIAMSINIMLS
                     QGGVRLGTGLGAPEFLTINVVVALISLLILLSRSAKVLSIDSALARRRPGLSPLLVNQ
                     RQRT"
     gene            13935..14420
                     /locus_tag="VFJ72_16135"
     CDS             13935..14420
                     /locus_tag="VFJ72_16135"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET7481035.1"
                     /translation="MGEERVPRREFIRYGAAVGLSVAGASVLASCGGGGDSAQSEEST
                     KKKSSSSGGTTDPTVGSGEPIVLAAFLPPGFAFPFTIAESGKPALLVHLKDDSWAAYE
                     AVCTHKGCEIGYQVDQPLLRCPCHGSGFDPADGAVANGPAQKPLQKVKVKLKGDNVVR
                     A"
     gene            complement(14514..14966)
                     /locus_tag="VFJ72_16140"
     CDS             complement(14514..14966)
                     /locus_tag="VFJ72_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481036.1"
                     /translation="MSAEKSGQGRIAVETGVGAYQITGARPERGSFARFTRPEGTSRA
                     ALEAACGIGVIVEAGVIPRLAGEQHYEAARREFGRPYAEGFAHGFDGAPVPREQWSRE
                     TDLERVIEGHRDGEALWAAVRDGVSPVPDFPPDEWSPETIPSGAPAAP"
     gene            complement(14966..15187)
                     /locus_tag="VFJ72_16145"
     CDS             complement(14966..15187)
                     /locus_tag="VFJ72_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481037.1"
                     /translation="MFPVEALSGIEKGDLPVETSLKIKAPGYGWRSVEARIFPRNGDG
                     VTEDGARSVGLSVPGYGNIPLSAVFGGAR"
     gene            complement(15437..16369)
                     /locus_tag="VFJ72_16150"
     CDS             complement(15437..16369)
                     /locus_tag="VFJ72_16150"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7481038.1"
                     /translation="MVELRHLRYFVAVAEELHFGRAAARLRVSQSPLSQQIIQLEREL
                     GVELLERTKRRVELTSAGEVYLREARRVLTYNERAEEAARRAGRGELGRLVLGFAGSA
                     AYGVLPLLLKASRERLPDLGIELRGELLSSELVRALGEDEIQIAILLRTSTENPDLEV
                     AVVRREPFVAALPADHPLARRRVVDPRELSEEPFVIFPRRRGAVLHDAVINTCQDAGF
                     SPRVVQEATELPTQVSLIAAGMGVALVPASMRHLRYHGVVYRSLRGHPPAPDTAVAWR
                     RGGETPTVRAFLQVVRAVADVADIPDGVPGVSDL"
     gene            16662..17201
                     /locus_tag="VFJ72_16155"
     CDS             16662..17201
                     /locus_tag="VFJ72_16155"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET7481039.1"
                     /translation="MERISRERFIRVGAALGVSAASASVLAACGGGDSGSSGGGNSGG
                     STGSSGSSGEASTVKSKDGSGETTKTENASGKASGGSSSGAIASESEVATGSAKSFKN
                     SGQPAVLVHLDSGDFVAYSAVCTHQGCTVAYKGGQLACPCHGSIFDPANGGSVVNGPA
                     QQPLPEIPVKVEGGQVVKA"
     gene            17273..17893
                     /locus_tag="VFJ72_16160"
     CDS             17273..17893
                     /locus_tag="VFJ72_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481040.1"
                     /translation="MDSSEERIGLNGAAVVLGVAPEEVRRFVNDGRLRAERTENGGYS
                     LNFAEVHALARELEVESAEEEVFEGEVIGEESDPRSRLPAVPFERYERLLQQGQDYKT
                     RLEERNRQMEDIRVERDAARVEIDRLWSEIERLNLIEFQRELQEKTISTLEEEKKKLE
                     AERERLINDRLELVEEKSGLVEERIRLEAELQALKNRGFWSRLFGG"
     gene            complement(17983..19131)
                     /locus_tag="VFJ72_16165"
     CDS             complement(17983..19131)
                     /locus_tag="VFJ72_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7481041.1"
                     /translation="MKALVPLVLTLLMAALAFASPAHAATVANGDFESGTLTGWTVVN
                     QNGGNGDWYAYSGTASPQSGRQITAPPQGNFAATTDQTGPGSHVLYQDIALEENAEHT
                     LSFILYYQNEAQGFSTPDTLDYSTGANQQYRVDILKPSADPFSVDPADILDSVYRTNA
                     GDPNTLEPTPMTFDLSPYAGQTVRLRFAGVESQFFFRASVDDIKVETEIPPPPPDPTP
                     DPKPACNDGRDNDADSKVDLRDPGCSSRSDNSEKNPTPDPNACTIKGNDRDNILKGTS
                     KRDVICGLGGNDIIKGLGGNDLIKGGAGNDTIQAGSGKDVVYGGEGKDAIQGQDGADT
                     LYGQENDDTLQGNGGNDKLYGSSGNDTLQGGLGRDVLRGGPGKDVTQQ"
     gene            19304..19564
                     /locus_tag="VFJ72_16170"
     CDS             19304..19564
                     /locus_tag="VFJ72_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481042.1"
                     /translation="MTTLCIQQPSGVRRDYFRVRQGAWLEATRGQVSVTMVFWKMAER
                     GLDLQGTDSAETRRNMEEMRDFYAHWERELPAIFERWEKEKQ"
     gene            19561..20469
                     /locus_tag="VFJ72_16175"
     CDS             19561..20469
                     /locus_tag="VFJ72_16175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7481043.1"
                     /translation="MSLAIETRNLTKTYGRKARGISEVNLQVAPGEVFGFLGPNGAGK
                     TTTIRTLLGFLRPTGGEASVFGLDIRKESVGIRARVGNLPGEFALEDRMTGEGLLRFF
                     ARLRGVKDLGYAHELAERLGAELRRPMRRLSRGNKQKIGLVQAMFHRPPLLILDEPTS
                     GLDPLVQEEFLDIIEEVRDEGRTVFFSSHNLAEVERVCDRVGIIRNGRLAAVETTETL
                     VDKSFRHVVLTFAESVDPKPFEALPGVKNVKFDGAKVSFTLYDNLDEMVKLAARHRLV
                     NLEYERPSLEEVFLTYYGNHRNGEGA"
     gene            20466..21323
                     /locus_tag="VFJ72_16180"
     CDS             20466..21323
                     /locus_tag="VFJ72_16180"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7481044.1"
                     /translation="MSAAQAVKKRAGRQRVERRAPRDALLALISHTVRMQLRGVLIWG
                     GILGLYSAAIVATYPTFGDMQGFDQFVQAYPKGLREAFNITDMSTIEGFLDTEIFSFM
                     APLALAFFPILAASSAIAGAEERGTIDVLLGNPIQRWQLVAGSFVATAVSLLGILAVA
                     GLFAWITAALVDVDLSVENAVNGYLNLWPICLFFGGLALLCSAIFHRRSLAIAVPGVV
                     LLGMYLMDVLGKVSEDLEDLRPYSLFYYYGSAIRDGIDWVNFGGITLVTLALIVLAMI
                     AFRRRDIYT"
     gene            21376..23052
                     /locus_tag="VFJ72_16185"
     CDS             21376..23052
                     /locus_tag="VFJ72_16185"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7481045.1"
                     /translation="MTERTNRDDRSAAFPTLARGQIEVLKRFGEVREIEEDEVLFKEG
                     DASYDFIVILEGLVEIRENFRGESRTVARHGAGRFLGEMNLFTGQAVYLTALVTEPGE
                     AIFIPPERLKEIVAEEPGLSELILRAFLMRRVILSGLGTGLKVVGSRYSEDTLRLREF
                     AVRNGIPHAWIDLEEDEGSEALLREFGVEPSETPVVIWQGTDVLRNPTNAELSRAIGL
                     GADVTPEDTYDLVVIGAGPAGLAASVYGASEGLSTIALDAVAPGGQAGTSSRIENYLG
                     FPAGLSGSELASRAMIQADKFGARFPGPREVVGLRRENGLYRVALSDGEEVAGRSVII
                     ATGARYRRLDAPRLEDFEGVSVYYAATEVEAQMCAGSEVIVVGGGNSAGQAAVFMAGR
                     TRKVYVLIRGGDLSRGMSRYLVNRVEGTPNIEVLTRTEVRELLGDEHLEGVIVEDNSS
                     GERRTLGARAVFVFIGADAHTEWLEDVLELDGRGFVVTGPSLESSDGVAWPLARDPFL
                     LETSLPGVFAAGDARSGSVKRVASAVGEGSMAVRFVHQYLLESRVEAVSG"
     gene            23080..24384
                     /locus_tag="VFJ72_16190"
     CDS             23080..24384
                     /locus_tag="VFJ72_16190"
                     /inference="COORDINATES: protein motif:HMM:NF017485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7481046.1"
                     /translation="MESRDLDRLLLRTRGLAVLRGAIDSPVARDFLALLRLLSVERPE
                     AGELAEVFGRLWEGLAMENERLLPDAWQSHLVGRILDDENSFSRGAELGGAAVAIRDQ
                     ARRDLRTLRTLFDLDAPTLLGMIEAAVPGLTSIWAPWTAPEPDSESARHTIARKLAAA
                     GDWDSCAELLEGHFARHGAGLFGGNRAFRWWEGSLHPVPRPDPVRLSGLVAYDRERRP
                     LIENTERFLSGLPAHHALLYGLPGTGKSSTVKALANEYADDGLRIVEVAKEDLSELPR
                     VLETLRGRGPRFILFVDDLSFEEHEVEYKSLKALLEGSVEEPPENVRLYATSNRRNII
                     RESFSDREDGFAGDDVHARDTMQEKLSLSARFGLRVTFPSPDQNRYLEIVSGLARERG
                     VEIPEGELRERALLWDRWHAGRSGRTARQFVDEIQAETSTRV"
     gene            24632..26131
                     /locus_tag="VFJ72_16195"
     CDS             24632..26131
                     /locus_tag="VFJ72_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HET7481047.1"
                     /translation="MLLVAILLTLASVATFAGRANALVQDSSPTVESPDKPNAEDDAY
                     SVDEDNTLVVAEPGVLGNDTDPNGRPLHAELKTGPANGTLDLNEDGSFSYTPAADFSG
                     TDSFVYESCNNIEPEICDEGTVTITVNPVNDPPFADDDSATTDEDTAVVIDVKDGDSP
                     GPADESGQSLTVDSVTDPAHGAAEIITTGGDAGKVLYTPDPNYSGSDAFDYTVCDDGS
                     PIKCDVGTVTVTVNQVDDLPEINLDEESARVVNEGDSRTYSYSVEDVDAPSQISESCG
                     ANATYTDTPDANSFECFFPDGPKTTQVSALVNVPQELSQASDTRVSSLEDLGARITVQ
                     IKNVAPRLSLTGSSKAKKGQTLPFRFTITDPGRNDTMSFGAGYPLCGQGARLASRPLI
                     GGGLLKCSYLKSGSRPVLSMQVKDSDGAMSNRVNHMVSVAAAKPAPKPHHDKPHHDKP
                     HHPKPHHDKPRPNCKDIPSKPGHVGSTHGGDPSQAGDARGSCSGHATAG"
     gene            complement(26232..27557)
                     /locus_tag="VFJ72_16200"
     CDS             complement(26232..27557)
                     /locus_tag="VFJ72_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7481048.1"
                     /translation="MTTPASGPKVINRREFLKLGGVGIAGVSLLGAAGCSKGEREGPA
                     KLIFTHGPDGAQIWQEQIDRFNRRHQGEIKVVLHQAPADTGQYFEMLRTGFQAGETRA
                     DVISGDVIWPAQFAAKGWISDLSNRFSPDLQSRHLPGAIASNTSDGGIYGVPWFTDAG
                     MLYYRKDLLEKSGFSEAPRTWDKLMEISQKVQRDQNIKYGYAFQGADYEGGVVNALEF
                     VWNAGGDVLGPKDRVVIGEPRAVDGLRAARGMVESGTSPEAVSSFKELEAYTVFLAGD
                     AVFMRNWPFVYALSSNPSLSKVKPEQIGLAPLPTARSGERSSSGLGGWNLMINAATHK
                     MDAAWAFIEYLSGSEQQKERALKGGYLPTLKALYDDSEILKEVPVAALGKEAIKNVRS
                     RPTSPYYYDMSLAISGRFHASLTGSISPKEEAAALQRDLQKILARAEKL"
     gene            complement(27554..29383)
                     /locus_tag="VFJ72_16205"
     CDS             complement(27554..29383)
                     /locus_tag="VFJ72_16205"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HET7481049.1"
                     /translation="MPSRIKGTGEIRQAVRDHLRRTAAAYGVLVVALLLTVLAALYVK
                     QNVEARENARFDETTSGVQRALDRRMDTYVDAMLEGRGLFAASNSVTREEWSRYVAAS
                     DLQRRYPGIRAMGYAPRVTLEGRDKHVSRLRGEGFPDYELRPAGERYEYFPITYVEPF
                     EGANRALFGRDLYAERVSRAAMEQARDTGLPRASGKIDLPSKKGSGGGPGFVVYTPVY
                     KNGAPHDNPDARRQSLQGYIVGVFGADELLAGIFGDQTSPGVDLEVFDGAELTSSGLL
                     HDDDGALHAADSSYSPPFEDTTTMEVAGRIWTLYFSSLPGFESGWRTNLPLFVLLSGG
                     AISLMLFGVTWMLSVNRAAAERVGRDLESANRELEATNQELTSANRELAATNAELQAF
                     SYSVSHDLRTPLRSIEGFSQIILEDYADRLDEEGRGHLERVKAASRHMSHLIDDLLDL
                     SRVTRKPLFRETVDLSALAREIAAELQAADPDRRVKLAVEDGLRAEGDTRLLRVALEN
                     LLGNAWKFTAGEDVAQVEFGSEPGSDGEEPVYYVRDNGAGFDMAYAGKLFGAFQRLHP
                     ADEFEGNGIGLATVQRVIRRHGGRVWAEGKPGVGATFYFTLPK"
     gene            complement(29388..29825)
                     /locus_tag="VFJ72_16210"
     CDS             complement(29388..29825)
                     /locus_tag="VFJ72_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7481050.1"
                     /translation="MSNKVILLVEDNPDDELLTLRALKKNNVMNRVVVAHDGVEALEY
                     LFATGSHTDPEVIPQLVLLDLKLPKVDGLEVLRRMRSDDRTRLVPVVILTSSTERQDM
                     LEGYGLGANSYVRKPVNFVQFTAAIEQLKSYWLVLNENPPVGA"
     gene            complement(29936..30361)
                     /locus_tag="VFJ72_16215"
     CDS             complement(29936..30361)
                     /locus_tag="VFJ72_16215"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HET7481051.1"
                     /translation="MQEPHNETERQAYPTTMSPQDEQTWSIISHLSVLAALIGVMPLG
                     ALIIWLVYRDRSERVRFHALQALWYQLAWVVLLIAYTILSLILSLVIVGIFMFFLLPL
                     IALVPLVHGCYAAYRVSQGEDYRYPFIADRIDGGPRRVA"
     gene            30813..32312
                     /locus_tag="VFJ72_16220"
     CDS             30813..32312
                     /locus_tag="VFJ72_16220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7481052.1"
                     /translation="MGNPLRVLVVEDSEDDTLLLLHELRRGGYQPFHQRVDTEQAMKA
                     ALREWEWDLVISDHSMPNFSSSAALDLLRSEGYMDLPFIIVSGQIGEDSAVLAMKSGA
                     QDYLMKDNLRRLTSAIERELREAEVRRERRLAEEAQRRAEEKYRGIFENSVEGIFQTT
                     VDGAFLIANPALARMFGYETAEELLRSVSSIGEQLYVDPERRAEFDRLVQRDGFVRGF
                     EIQMYRADGSVMWASVSARAVRDEETGEFAGFEGTLEDITARKNAEEALREIREFERR
                     RIARELHDVVLQDLTYSLQSMQLSRAVAATAGSVGGPGRGSGSEADEERDRQIEALRR
                     AVKGLRDAIYDLRLEGVREQPLVRSLEALVELNRQMVPDREIELAVDEGFPDSIAGVS
                     GVEITRIAQEALANIRRHSGARRVKVHLSSSGEEVCVSIEDDGRGFAPGTSPGMGMTG
                     MRERALALGGTLDVSGEPGAGTRVCFRADLSTLMEAPVDAAGGKRQPGV"
     gene            32390..33037
                     /locus_tag="VFJ72_16225"
     CDS             32390..33037
                     /locus_tag="VFJ72_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7481053.1"
                     /translation="MLVEDHASFRQTLALMFDRETEFEVAAQAGSLGEARQVAVEFGD
                     AMDLGVIDLTLPDGEGIELIQEIREVSPHFSALILTASLDQADHARAIEAGAAGVLHK
                     SADVDEIVGAVRTLAEGKTLISPEEIVTLLRLAGSNREQEREARAGIERLTPRELQVL
                     QALAEGLSNKEIAERLHVSVDTERTHMMNILTKLGVHSRLQALLFAVKYDIVELT"
     gene            complement(33059..33838)
                     /gene="dapF"
                     /locus_tag="VFJ72_16230"
     CDS             complement(33059..33838)
                     /gene="dapF"
                     /locus_tag="VFJ72_16230"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET7481054.1"
                     /translation="MRFTKMHGVGNDFIIFDPGEVEGVDLPDLARRVCDRHFGVGADG
                     ILVPTPSEVADIEMVYLNSDGSVSEMCGNGIRCLARYARDFEMVEGDALTIGTGAGVK
                     KVVLREDGSSRVNMGPPVLGTEVEVGGFALLRVSMGNPHAVAFLESAAAVDALELRDV
                     GPLIENDPTFPEKTNVEFAFARGRRDVRMRIWERGAGETLASGSGSCATAVAAVSRGL
                     AESPALIHLDGGIVEIEWAGEDEPVYMTGPAEYVCEGELLL"
     gene            complement(34120..34425)
                     /locus_tag="VFJ72_16235"
     CDS             complement(34120..34425)
                     /locus_tag="VFJ72_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HET7481055.1"
                     /translation="MLVFVDVRVDPKDMSLDELWEAWEEEANAAIGAIEAGKIVGLYK
                     VSGQRRVLGVLDVESHDELDQILMAGLPMAHYLTMAEVLPVREYTAFADDVRRRWQQ"
     gene            34745..34921
                     /locus_tag="VFJ72_16240"
     CDS             34745..34921
                     /locus_tag="VFJ72_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481056.1"
                     /translation="MEDQREVCLEEEVWRLRRAGNKPEEISARMGVDVSWVEQVLAML
                     PDEEYPGESANQER"
     gene            34958..35749
                     /locus_tag="VFJ72_16245"
     CDS             34958..35749
                     /locus_tag="VFJ72_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7481057.1"
                     /translation="MPNENPHRSVRRRRRTEDERLLSASGLDLAAFTETDTWRVFRIM
                     GEFVQGFEELADLGPAVTIFGSARVSAGDAMYETATEMGRLLGEAGFTIITGGGPGVM
                     EAGNRGARQAGAPSVGLNIELPFEQHVNPYVDIEVDFRYFFVRKMMLVKYSQAFVIFP
                     GGFGTLDELFEALTLIQTGKVRNFPVVLFGSDYWRGLLDWLNTTMVTEGKISSADLGL
                     LAVTDSPAEARDLILDSVREQQWSAEHEEGAREATREAFGEKPGS"
     gene            complement(35746..36534)
                     /gene="dapB"
                     /locus_tag="VFJ72_16250"
     CDS             complement(35746..36534)
                     /gene="dapB"
                     /locus_tag="VFJ72_16250"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET7481058.1"
                     /translation="MAIIGTAGRMGRELCRAALETDGIELAGGTVEPGAPELGADLGE
                     LCGAGKVGVTASEEPPENAEVLVEFTTPEATVAHLSYGKPHVIGTTGLSEDQMSQVAD
                     AAKSAPVMLAPNMSVGVNLLREVVRELSAKLGSGYDIEVVEAHHRNKKDAPSGTALFL
                     ARAAAEGRGQDLDEVATYGREGLAPRKEGEIGIHALRGGAVVGEHRLIFYSGGEEVEV
                     IHRALSRRTFADGALRAAKFVAGAEPGLYSMGDVLGIGGPETGD"
     gene            complement(36548..37444)
                     /gene="dapA"
                     /locus_tag="VFJ72_16255"
     CDS             complement(36548..37444)
                     /gene="dapA"
                     /locus_tag="VFJ72_16255"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET7481059.1"
                     /translation="MFSGAFTALVTPFRNGEVDVEALENLVEFQIQHGIHGLVPCGTT
                     GESPTLSESEDRLVIETVVRVANGRVPVIAGTGSNNTDSAIKYTKMAEDVGADGSLQV
                     APYYNKPTQEGLYRHFAAIAENTDLPLILYNIPGRTSVAIEPETIARLYEEVPNVVGV
                     KEATLSTGIAGEIKRLCGEEFDLLSGDDPMTLPLMALGGSGVIAVTSNIAPGIYADMV
                     NALLEGDFARGREIHYQLLPLFKALFVETNPIPVKTAASLLGLCSDEMRLPLMPMSGD
                     KLRGLEEALERHGHLLPTPEEV"
     gene            complement(37437..38666)
                     /locus_tag="VFJ72_16260"
     CDS             complement(37437..38666)
                     /locus_tag="VFJ72_16260"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET7481060.1"
                     /translation="MGIVVQKYGGSSVATAEHIKAVAARVGRARESGLDIVVVVSAMG
                     KTTDRLLRLAGEVSREPSPREIDQLLATGEEQSVALLAMALHDRGVPAVSLTGPQAGF
                     RITGRYGSGVISEIQPKRIREYIGEGQAVIVAGFQGMNALGDVMTLGRGGSDTTAVAL
                     AAALEAERCEIYTDVDGIYTADPRIVPEARRIPVISSEEMAEMSWRGAKVMHPRAVEL
                     GALYGVEIHVRSSFDEEPGTAIVGGKHLEHLETRETVAGIVHDLDVSRITLNRIRTGP
                     GTMSRVFAPLAERGISVDVIVESGLEGGLSDVAFTVSRGNFAEARKLAAEVADSLGGA
                     VEGEEDLGKVSVVGTGMLNRPGYAARMFSSLGEAGIPIRMVSTSEIQVTCVLPAERVE
                     EAVRRLHKDFELEERDV"
     gene            38902..40572
                     /locus_tag="VFJ72_16265"
     CDS             38902..40572
                     /locus_tag="VFJ72_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481061.1"
                     /translation="MRFTSVRHAAGSGGYGRGLFLGVAVSVVMFVGIISHAAQAQTPE
                     IEAKQAEVTQAQARIDATLEDLNKAQAAYDESQDKLAKVEGDISKNQDALKKTQKRLD
                     DAKEKLSDRAVGSYKSGGVMYLDVLLSVKSFADLSNKGQFILQVLKQDRDAVIKIAKI
                     KDDLAGQQRALKEKKQTQAGISDQMRKQQAAIDDRLAEQRATYASLSGEVQRMIQEEQ
                     ARQAREAAERLAKAEAQANAKKAAEQEAQAEKQAAAQATRQQMAQANAGEQASAEAEK
                     QALAETTARQQRQQLAVQADEADKRAEKAKDKTEKQRLAAEAEQARKKAAQQAAAEKA
                     ARQKQQQLIEQAQQAAQAAKQEAKQAADQSDQQSDQSAAADQYASGGESAAQDQYQDS
                     SSGGETTDASSAEPDPAATSSDPGAQAILDNPNVSLTSMAQQDLSAGIVDSRVLDVVA
                     FAAQEHSIAISVFKTGHPYGPTLDAIGLAGYPNSHYYGRAVDIYEVDGVPVSSGNQAA
                     HDLAQSIEDQFAPAELGSPWVFGPGSFTNEMHQDHIHVGWAYSADGGL"
     gene            complement(40695..41591)
                     /locus_tag="VFJ72_16270"
     CDS             complement(40695..41591)
                     /locus_tag="VFJ72_16270"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7481062.1"
                     /translation="MTDQHGQQDPREKYPQPEFPEQDQRDQHPGLESEMEPKPDYGYE
                     TYRGSGRLEGKKAVITGGDSGIGRAVALAFAREGADVLISYLPEEESDAEETARAVEE
                     SGRRCVRFPGDISEEATCVSLIEKAVEEFGRIDVLVNNAAHQMTVGSISEVSTDLLDR
                     TFKTNIYAMFWTVKAALPHMPKGGAIINVGSAQAYKPSPPLLPYSATKGAIVTFTEGL
                     AQEVVEYGLRANVVAPGPVWTPIIPASMPGDTVSSFGQQSPTGRPGQPVEVSPAFVFL
                     ASDESSFVNGAVIDITGGQPVA"
     gene            41760..44087
                     /locus_tag="VFJ72_16275"
     CDS             41760..44087
                     /locus_tag="VFJ72_16275"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HET7481063.1"
                     /translation="MGEYEAAFQNQPVDISGQFARQENHFFLGEKVESFDPLMASGEI
                     IWKSLSLKQRVSYHQLTLQFEDYKVWEDTPPGEYEDDQVLPFDISFVSPRTVRLRVAA
                     RREPIREKDSLMLVGEPGSDGSWEMSDEGGRVTYTAPHGSVVVEREPFHFEFRDASGR
                     LLTRTNHLSDTRSVVNSMPTPFSFVRNASNLHRHIAASFKLSPGEKLFGGGESFTRLD
                     KRGQKMVLYTYDAYSAQTPYMYKPVPFFMSDRGYGMFVHTSAPLTFDLGGSYDGANVI
                     YLGDDFLDLFVFIGSPKEILTEYTALTGRAPTPPLWSFGLWMGRESYSSEQETREVAR
                     KLREHRIPSDVIHLDTDWTETPHRCDYEFSPSRFPTAEKMLSDLREDGFRVSLWQLPY
                     LNPMNELHAEAIEKDLVVLSANGKEPVDDAVIDLGKPEAVEWYQGKLKKLLEKGVGVF
                     TADFGEAAPLSGIYAARHGGFHEHNLYPLRYNKAVAEITEEVNGYGVIFARSGWAGSQ
                     RYPLHWGGDAENTDTALAATLRAGLSLGLCGFSFWSHFIGGFAYQSPADLYLRWLAFG
                     MFCSHSRCHGAPPREPWEYGKEFMDGFRRIVETKYRLMPYVYAQARLCSERGHPMIRP
                     LFFEFPEDRTSWLIEDEYLFGEDILVAPRLEDAPGRDVYLPPGFWIDYQTGEVYEGAR
                     WHDIRAGDLPIVLLVRDGAAIPHAGLAQSTEWIDWSEIELRVYGAETRAARGLFCLPE
                     DGESHVLRLAREGGSFTLAEDPLPHRVRWSVNVYP"
     gene            44191..44694
                     /locus_tag="VFJ72_16280"
     CDS             44191..44694
                     /locus_tag="VFJ72_16280"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HET7481064.1"
                     /translation="MSEDERPEGIFEGYDKNDEVEISSYAALVGLFNLLFALFLFLAR
                     RTGRPIPERIETRDILLLGVATHKLSWLLAREPITSPIRAPFTELEEVKSPTKADEKP
                     RGTGLRRSVGELLTCHFCLGMWVASFFSYALVLFPAATRLVGAIFAMLTVSDHLHQTY
                     KALMDRT"
     gene            44829..45266
                     /locus_tag="VFJ72_16285"
     CDS             44829..45266
                     /locus_tag="VFJ72_16285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7481065.1"
                     /translation="MSEGHILFVDDRPQNAALFSRVLKIHGITNEVIPAGDGIEALEY
                     LFGTGQHAGRGTRMPRLIILDMRMPRMGGLETLRRIRADRRTEIVPVVVFSSSDDPQD
                     MLEAYRLGANSYVDKVSLNLPYPEIAGMIARYWLLVNEPPPGT"
     gene            complement(45290..46822)
                     /locus_tag="VFJ72_16290"
     CDS             complement(45290..46822)
                     /locus_tag="VFJ72_16290"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7481066.1"
                     /translation="MSGRYDAIVVGAGPNGLSAAIRLAEAGHSVLVLEAEDRPGGAVK
                     TEALTIPGFLHDTFSGVYPATAASPVFARMPLGEHGLRLVHPPVAMVHLLDESRGAAL
                     YRDLDRTAASLDDLTAGDGEAWRRFISPYLKVWPALRRAMLGGFPPVGGSLRTAAGLG
                     LTGSLETARMILQPVSALADELFEGDGAKAWLYGSALHGDVAPDASGSAVFGFYLNLL
                     GHAVGWPVAEGGSGRITAALVSYLNSLSGELRVNAPVERIVAENGRMRGVEVGGEIFE
                     AGVVVCDVTPQGLLGISAHALPDSYKRKMARYRYGPGSLKLDWALSGPAPWSYETARG
                     AGTLHIGGGTRELSEGIATEKLGGLPESPFLLAAQPTVADPSRAPEGCHTLWAYTHVP
                     SGADPASLEGHVERIEAQVERFAPGFRDLIQGRHVLTPADLESRNCNLVGGDVNAGSQ
                     APDQLFFRPLPSLSPYRTPLRGLYIGSAATFPGGAVHGVPGDSAAKAALTDLRLRRVR
                     LA"
     gene            complement(46826..48202)
                     /locus_tag="VFJ72_16295"
     CDS             complement(46826..48202)
                     /locus_tag="VFJ72_16295"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7481067.1"
                     /translation="MQGVGVGGLEGRLDLQPLNPGHWRVTLLSGFGWMFDAMDIGLLS
                     FVMVVLAQEWGLSEGQVGLAISTGLFGMFVGAAVSGALSDRYGRKTLLMTTLLVYSVA
                     TGLTALAWGFGALLVLRFVTGVGLGGELPVASTLVAETAPARHRGRLIVLLESFWAYG
                     WILAALIGYLVIPEFGWRIAFVLGSLPALYVLVLRRGLPESPRFLLRKGRYGEAREAA
                     RRLGIEDTGPDREVTDEEAGKTGIGALWATSYARRTAMLWVLWFGMVFSYYGIFTWLP
                     QILATSGYGVVKSFEYVLLITLAQIPGYFSAAYLVEKWGRKPTLVSYLLCCAAATLVF
                     GLRGFGAETSGAELVLWGSLVSFFNLGAWGVIYGYTPELYPTAVRGSGAGWAAGVGRI
                     GGIAGPYIVGMMLGAPGFGTLAVFSMFAVVLFVIAADVWLLGEETRGRTLDEISMPAR
                     RAATASGS"
     gene            complement(48354..49397)
                     /locus_tag="VFJ72_16300"
     CDS             complement(48354..49397)
                     /locus_tag="VFJ72_16300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7481068.1"
                     /translation="MPEVKGPASLLLEERTGRLRPRAVWRMLLQYLGFRVLIALLVNL
                     LAVASLLLLSGGRAFSSGAVDTSLLSDSPLVPLISAAASLSAALLSVWLAGRLLDRRP
                     FADFGFHINVRWWLDLLFGLLLGATLMTVIFLTELALGWISITATFESVYAGTPFAQA
                     VLVPVVVFTGVGVYEELIFRGYQLRNAAEGLNYPMLGAKNAVLLAWALSSIFFGWLHS
                     GNPNFPAIGLLNITVAGLMLGLGYVLTGELAIPIGLHITWNFFQGAVYGFPVSGLDTI
                     GASFLTVKQSGPVLLTGGAFGPEAGLIGLSAMALGGLLIVLWIKLRHGGVELHTPLAE
                     PSESGRPSAPGPE"
     gene            49575..50144
                     /locus_tag="VFJ72_16305"
                     /pseudo
     CDS             49575..50144
                     /locus_tag="VFJ72_16305"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(50164..50895)
                     /locus_tag="VFJ72_16310"
     CDS             complement(50164..50895)
                     /locus_tag="VFJ72_16310"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HET7481069.1"
                     /translation="MAEISVGEKAPTFELPDQTGYPWNLSGQLELGPAMLVFYRGDWC
                     PYCNGQLASLARGFERFARYNTEVAAISVDPPEHSLALSNKLLLPFPVLTDPKGELAK
                     RYGLWDAKEGVAVPATVLIDRTGEVAYLYAGKDFADRPAEEEVLSVLKRLKNTHKHGL
                     EGPEVQVNAVDSHDSVRPDRPAMTLDQLIPYYQGVFFSTVALGGRFSGWGRSGRHASG
                     EVSNYRTTTSRYAEALRETIRLHES"
     gene            51003..52766
                     /locus_tag="VFJ72_16315"
     CDS             51003..52766
                     /locus_tag="VFJ72_16315"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HET7481070.1"
                     /translation="MCWKKRGTILDLQFTFYTLALTAGAAVAAALAFYSWRRRGSSGA
                     RELALLMAAVSVWSLCYGFEFLFTALNAKLFWASVRYAGVVTVPVAWVLFALRYTGRE
                     RWISGRNLALLAVVPAVTLALVWTNWAHHLFWLHATTDTTETFLLVEQGAWFWVFFAY
                     SYLLVALGAFFLVSMFVRSPGLYRGQGGALLVGALAPWVGNGMYFLGFWPLPNLDPTP
                     LAFLVSGIAVSWSLFRYRLLDIVPVARDTVIERMNDAVIVTDTRRRVVDLNPAARRLL
                     GAQGTGTVGSPLALLAPDFDALLEGYGEADEAHKEVPFGDGAEGREYEMSLSSLRDRR
                     GYLTGHLLVLRDVTERKLAERELLRQRADLARTNAELEHFAYLIAHDLRAPLRAINGF
                     SQILLEDHGDTLDAGGRDYLTRVADAARHMGHIIDELLDLSSLTRAELRRETVDLSAL
                     VRGISDELRQGDPTRAVTFDIQRDLTAECDRRLLRIALANLLDNAWKFTSRQPEARIE
                     FGTEERDGAVVYFVRDNGVGFDMAHAKKLFGAFQRLHSASEFEGAGIGLAAVERVVER
                     HGGRVWAESDIGRGATFYFTL"
     gene            52995..53849
                     /locus_tag="VFJ72_16320"
     CDS             52995..53849
                     /locus_tag="VFJ72_16320"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET7481071.1"
                     /translation="MLYSLPLRAPSRQPQEGPGRAPERTPGRIPDQTPNRKPRRRAFG
                     RKISRKKIYRRRRRAALAIILVFMLAVAWMLWRSGEDATTAGPAPIPQSEVRKSETRQ
                     SEAPAKSGTATAEPARAAGDPDVWGSKKKDAEQKLPPPPADKTLYLTVPKMGRYDDVV
                     RNDDSEWALDNGAIKLPTSGFPWQPSANVYIAGHVLGYAGTGSYMQFANLPSMVKGDE
                     IFLTDANGTTYEYRVTEILTVRPDENWVTAPVAGKDLVSLQTCINPPNYDHRLIVRGE
                     RVGVKTTA"
     gene            54038..54622
                     /locus_tag="VFJ72_16325"
     CDS             54038..54622
                     /locus_tag="VFJ72_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481072.1"
                     /translation="MKKLSMSLLLAVVLALALASVAFAQGGTHQREFRAKLATLNQSG
                     ATGVADLTLRGRRLEAEIKTRGLEPNLHHLQHIHGMAQAISECPTLAADTDGDGLVSV
                     AEGAPFYGPIQVSFTETGDTSAASGGALDRFPLANAKGRLDYSRTFRIPADVAANVGD
                     FAIVQHGVDLNGNGSYDNAMEVSLPATCGKIVRN"
     gene            complement(55054..55236)
                     /locus_tag="VFJ72_16330"
     CDS             complement(55054..55236)
                     /locus_tag="VFJ72_16330"
                     /inference="COORDINATES: protein motif:HMM:NF017353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET7481073.1"
                     /translation="MSDKSSNQDRAEGAVDKAKGRAKEAGGALTGDKDKKAEGRGDQT
                     KGTLKEKKGAAKDLLK"
     gene            complement(55523..>56479)
                     /locus_tag="VFJ72_16335"
     CDS             complement(55523..>56479)
                     /locus_tag="VFJ72_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7481074.1"
                     /translation="PDPGIWWELVERYGVATMFTSPTAIRALRKQDPEYMRRHDTSTL
                     RKLFLAGEPLDEPTSQWTSETLGTSVRDNYWQTETGWPILSSMLPGLEDTPVKAGSAG
                     FPCYGYDTRLIREDTGEEAGPGERGVLAIAPPLPPGCMSTVWGDDERFVQTYFSDFKE
                     QLYSTFDWAMKDEEGYHYIMGRSDDVINVAGHRLGTREIEEAINAHDDVAEVAVVGVK
                     DEDKGQVVFAYAVPKDGDKVESEAGRSELEQSIKEIVAKKIGAIARPSAVYFVEALPK
                     TRSGKMLRRGIQALAESRDPGDLSTLEDPAALESVRASNTGE"
BASE COUNT         9694 a        18364 c        18554 g         9867 t
ORIGIN      
        1 agagagcggc gaggtcatcg tcctggacgg ggcgacgggg acggagctgg agaggcgcgg
       61 ggtgccgatg gacgacgcgg catggtgcgc ggccgcgctc gttacgcacc cggaggtcgt
      121 gcgcgaggta cacgaggact acatccgggc gggcgcggac gtggtcacca ccaacacctt
      181 cccgacggcg cggcacgtcc tggagcccgc cgggatgggg gaacgcttcc gggagctgaa
      241 cgcgctggcc gtggagcttg cgaggcagtc gcgggagaat gcggcgggct ccgccggccc
      301 ggtgtacata gccgggtcga tctccacgtt ctccgcccgc cacgataata gctacgagcc
      361 ctccgagaag acggcgcgag ccaactaccg ggagcaggcc gagatcctcg cggaggcagg
      421 ggtggacctc atcgctctgg agatgctgcg agacatcgag caggcaggat acgcggtcga
      481 ggcggccgtc tcgacggggc tcccgatctg ggccggcttc agttgcaagc gggccgcgga
      541 cgggacggtc gtgctatggg acggcgggca cacgctcgcg gaggcgttgg cggagatacc
      601 gccgctcggg gtgtcgctgg tctccgtaat gcacacgcta atcgaggatg cggttccagc
      661 gctgcgcgag gtggtggagg gctgggatgg accggtcgga gcgtatccgc acgcggggaa
      721 gttcattatg cccaactggc agttcacgga catgatctcg ccggaggact tcgcggagag
      781 ggcgcggggg tgggtagacc tgggagcgcg tgtaatcgga ggctgctgcg ggatcgggcc
      841 ggatcacatc cgggcgatca gggagagttt tccttcgagt gtcgcggcgg gcaggtagcg
      901 gtggtcagat ccaaacggca caatgaagcg tatatatctt cggatatggt gagggagaga
      961 atgctggcgc aacgcctcca ggcgtctcga tccaagactc ggccgcgggc ctccgggagc
     1021 cggacagcgg tggctatcgg tctggccacg gtaaccgtcg cgtggctgat cgtcaacctg
     1081 ctcaccggct ggacgcattt cgcgttgctc gtgaacccga agctgagcgg cggcgaggag
     1141 gcggcgtcgg cgctgacccg gttcttcggc gctctggttc tcgtattgtt ctttgcggag
     1201 aacgagggct ggcgtctgcg atgggtggct gccgggcttg tggtactcgg cctgggacac
     1261 ctgaccttcg gatatctgga gcctacgatc cagaacgacc cggccgaact gaacgaatcg
     1321 ctctacgagg ccctggtcac ccagatcctg gcgtgcgcgc tgttcgttat cgggctggtg
     1381 ccccggcgat cgccccggct gcggacgtgg gtcccgctgg taatcctcgc ttgcgtgccg
     1441 ctagccggct acatttacat cttcgagctt ctgcgggacg agacctggat gccagcgttg
     1501 accgtcctgg agagcgcggc ggagaccgtg gaggccggaa cgccccccga gtggctcacc
     1561 gtgtggcact ggatcttctc ctcgataccg ttagttctgg cgacgacggc cgcggtcgga
     1621 ggcatcatgc gtagccgggg gagcggtggc gatgtgcgga gagcaccctg gtggtggctg
     1681 acgccggcca tagtgctgct tgccggcgcc cagttgcacg aggtcttctg gccctccggt
     1741 tacgcgggga gggtactaac gacggcggag ggtctgcgac tcgccttctc ggtggtcgtg
     1801 gtcgtgggcg gcatcataga gctctggcgg atcggctccg agcgggcgac ccttctcgcc
     1861 gccgaggagg agcggcgtcg gcggctggag gagctcgcgg tgatgaaggc cgacttcagc
     1921 gcgatggtcg ctcacgagct cggttcgccg ctcgcggcca tccggggata cgccgacatg
     1981 ctggccgcgc cggagttgga accggacgca agatcctccg cgctgaaggc gatacggggt
     2041 gagatctccg cgctgaacac tctggtcgac gacgtgcggg acgccgccac gggagagcgc
     2101 gaagacttcg ccgtagagcc gcgtccggtc tctctggaga cgctcctcac ggaagccgcg
     2161 gccttcgccg cgaccctccc cggaggacac gagctcaccg tcgtccgcga gaccggcgcc
     2221 agagtcgtcc gggccgacgc ggagaggatc gggcaggtac tcaggaacct gctttcgaac
     2281 gcggccaagt actctcccga gggcgcgccg atacaactcc gggccgtgcc gggtggcggg
     2341 gggcgcgtgc gcctggaggt tgcggacaag ggtcccggca tagaccccgg cgacatcggg
     2401 cgcatcctgg agaagttcgg ccggggcaag gacgccgccg gcaagaaggt cgccggcgtg
     2461 ggacttgggc tgtacctcag ccgccgcatc gtcagctctc acggctgcga gctcgaggtg
     2521 cggtccacgt ccggggaagg ctccgtgttc gcgttcgagt tggaagtcat cgaggaggga
     2581 gacgagcagt gatccgggta atgctggtgg atgatcacgc ctccttcctt cagccgctag
     2641 ccttcatgtt cgaccgggag ccggatatca tggtcgtggc gcgggcggag tccgtcgcgc
     2701 aggcgcggga gaggatcgaa gaattgaagg cggcggactc caacgtggac ctggcgctcc
     2761 tggatctcga ccttccggac ggctccggaa cggaggtgat ctcgatcctc cgggacgcca
     2821 accccgaggc cctcgcgctc atcctgacct ccttctccga gcgggatcaa cttgcgccgg
     2881 ccatcgaggc gggggcggcg ggcgtcatgc acaagtcggt cgagatctcc gagatcgtgg
     2941 acgccgcgcg tcgcctgcac gccggcgagc agctcatctc gccttctgag gtcatagagg
     3001 cgatgcgctt catcggccgt cgtcgtcagg aggactatga ggcgcaacgc atggccgaga
     3061 gtctcaccca gcgcgagcgg gaggtcctcc aggccctcgc cgacgggctt ggggaccggg
     3121 agatcgccga ccggctccac gtcggtatcg gcaccgtcag gactcacatg gtcaacattc
     3181 tcagaaagct ggacgtggct tcccggctcc aggctctact cttcgccgtc cgccacgatg
     3241 tcgtgacgat aaattagata gactagccgg cgaaccggcg aaaccatgaa tccataacgc
     3301 atctatatag aaaaatttat acataaaggt agatgcgttc tcagcttccg cggagtaacg
     3361 tgtagcggat gctttcgatg gatatgaccg aacatactct gacccctgac gctggcgccc
     3421 ccgaacggcc gcgtgttctt gttgcgaacg atcccggagc ttaccgggac gctatctcca
     3481 gggccctgcg ggtgatgcgg ccggaagtgg aggtcctcga tatcgcgccc gcggaactgg
     3541 accgcgaata cgctcgcctt cggccggctc tgaccgtgtg cagcaagctc acgggagccg
     3601 tagaagcggg gccctcggat tgggtcgagc tatatcccga tgggggttcc gtcgcccacc
     3661 tcagcgtcgg gggggagcgc tccacccttg cccggcctga cttctgcata atcctctcac
     3721 tcgtggaccg gcttctgtac cggacctgat ccacggttcg tgttcggagc ctgccagcct
     3781 gtctaaaagg tattttcggc ttagaatatc gtgtgcggca gggtagccgc gcagcgaaaa
     3841 aggccggagc catgaagaag ccggagaggc tagcgaggac aagagccagg aagcgggcca
     3901 gggaccggac gccagcgcgg gaagctgcgc caacgcccat attcgtatca cctcgcgtgc
     3961 tggcactcct ggtcgggatc atcgtggtgg tgttcgcgct ggtgatctac gcggccccga
     4021 tcatacccgt cgtcgcgctc ggcgggatgg cgctggctat cgtgctctcg ttccccgtgc
     4081 gggcgctctc tcggttgatg ccgcgggggg tggcgatcct ggtgacgttc ctgggtctga
     4141 tcggtctggt cactctcgcg ctcgtcttcc tggtgccgct gctcgtgcgg cagctcggga
     4201 acttcatact gatcgtgccg accatcgcca acgatgtcaa caggttcctg ctcgacctga
     4261 tccaaccgct ccgcgagcgc gacgtgtttt tgatcccctc cgaccaattt atgcagggcc
     4321 tggtgcagga cctcttcggg cgcgcccagg aggtcattca gggtatcctc ggcggtctcg
     4381 tcggctttat ctccggggct ttcagcttcg gcatagctct cttcggcgtc ctgttcgtgg
     4441 ccgtctatct gctggttgac gtgcggaagg tgaaggcggc cttcttgaag gcggcgcccg
     4501 tacactaccg ccacgacgcc cgcgagctct gggacgcttt cgggttctcg ctctcccgct
     4561 acctcggcgg tctgctcctc gtggtcgtgg tccagggcgt gctcgccacc ctggcgctcc
     4621 agattctggg cgtaccatac gccatcctac tcggcgtgtg ggtctcgctg acggccatca
     4681 tcccgtacct cggccccttc ctcggcgcca taccagctct gatagtcgct ctcaccttcg
     4741 ggtcgccagc tttcgagaac aacacgacga ccgccatcct cacgatggtc gcatacgtcc
     4801 tgatccagca gttcgagggc aacttcctga ccccgcgcgt ccagggccac gcactgcacg
     4861 tccacccgat cctggtcctg ctcgccgtga taggcggcgg acaactggca ggactggcgg
     4921 gcgtcatctt cgccgtgccg accctcgcca tcctgcgcgt cttcttcgat ttcttccgcg
     4981 cgcgcctccg cacgcccctc gcgcgagccc cccaggagcc cgaactctaa agatttctac
     5041 tcctggctct tcccctgctt gacgatgtcc ttgagttcgc tgtcgagcgt cttgatggcc
     5101 cccttcgggc tcgtctcgcc gttgagcgag ctgttgaact gcttcgccat cgccagggat
     5161 atatcggagt agtaggcgga gaccggacgg ggacgggtgc gcgagatggc ctccttgccg
     5221 agcttgatga ccggcacctt gcttaccagt tcctggtcgt ccagcaggtc cttgagcgcc
     5281 ggtatccggg ccgtcttgag cgcgaactcc ttctcttgag ccggggcggt catgtactgg
     5341 atgaagctcc aggcggcgtc cgggttcttc gatttagcgt tgatcgagaa gttccatccg
     5401 ccgagggcgc tgaagctgct gtcgccctcc ttgccgaccg gcagcggcgc gaccccgatc
     5461 ttactttgct taaccttgga ctcgctctca tcggcggcca gaccgtagat gtacggccag
     5521 ttgcgcatga acaccgaatc tccgttaagg aacgtcgtct gcgcttcctg ctccttgtaa
     5581 gagctcacgg ctcctggagc gacaccggca tcgaccatgc tgcgctccgc ggcgagtccc
     5641 gcgattgagc cggggctgtc gaccacgacc ttgttggggt tgtcggggtc caggacgtct
     5701 ccgccgtgcg tccagatgta ctccaggccg ttgcagaccc cgccctcgta ctgggctccc
     5761 tggaagacga acccgtactt ggttcccgaa tcttgcatga ccttcttcgc catctgctct
     5821 agttcatccc acgtcttggg gggatcgctg aagccgctct tctccaagag gtccttacgg
     5881 tagtagagca tcccggaatc ggtataccag gggacgccgt acggcttgcc cttgaaagtg
     5941 ttggcctgca tcggagccgg gaggaacttg ttgcgcgcgc tctcggagaa acggtcggag
     6001 aggtccaggt agtagccggg agcggcgaac tgggccggcc agactacatc tccgccgatc
     6061 acgtccatgt cggtactgcc ggcctggaac tgggtgcgca gcttgtcgaa gtgctgggtg
     6121 ctgtccgtgg gcatctcctg gaactttacc ttgatgccgt cctcgttctc cttgttgaag
     6181 cggtcgatca tcttctgcaa cgtcccggtc tgctccggta tccacgagaa cgtgatgctc
     6241 ccggaccctc cgcccgcgcc gctgccgcca ccgccgccgc aacccgccag cgcgccgctg
     6301 ccgaggagcg ccgcgcccgc cagccccgcg ccgccgacct tgagaaattg tttccgggtc
     6361 atgccgtccg tggcgcgacc gctcccgcca tcgttcgtcc gcgaatgcgt catgcttgcc
     6421 ccttccttcg ccaccaatcc cccgccggct cgccggccct ccaattatga cgcatcagcg
     6481 gatcgaatgc ttcagggatg cttgaggaac gagaccagac gctcgtagca ctccgcgagc
     6541 agcggaagcc ctgcgtactc cgcctgctgg tgggcctgcg acgggagccc cggcccgtag
     6601 ttcgccgcag ccaccccgcg ctgcgcgaag cgggccacgt ccgtccacgc ctgcttcggc
     6661 cgcacctcca ggccgccctc cacgagttct ctgagcagcg aattgtccag gctgaccgga
     6721 ccgctcgggg cgaagtccgt tatctcgtac gaggcgccgc cgtccagtaa gctgtcgaag
     6781 aggcgcttca cgtcttcgat gtccctgccg ggcgcgaagc ggtggttgac gttgatccag
     6841 aactcgtccg ggaccacgtt cttcgcccgc ccgccgcgcg ccatcgtcgg cgtcaggacc
     6901 tcgtagaacg gcagcccctc gacgatgacc tcgttcgccg ccctctcgtg cagcgccgcg
     6961 aggaacgcgc ccgccttcgt cagggcgttc tctccctgcc agggccgggc ggcgtgggcc
     7021 ggtttgcccc gaaacgtgac ctccacctgc gccgtcccga cgcagcccac ctccagcgcc
     7081 ccggccgtcg gctccgggac gagcgccagg ttcgcgtcca acacccacgg aacctcggcg
     7141 aagacggtct ccagaccgtt ctccgcgtag ggcccctcct ctcgctcgta gaagacgaag
     7201 accggctcgg cccggcacgt ctcccactcc agaccttcga ggagcgccag catcaccgcg
     7261 tcgccggcct tcatgtccga ggcgccgcgg ccgtagaccc tgtcgccctc cacgcggacc
     7321 tcgatgccgc ccgccggctc cggcaccgtg tccaggtgcc cggcgaagac gattgtgggc
     7381 cgcgaggcgt cccgctgcgc gcggcggacg atcaggttgt tcgagacccg gcgtgtctcc
     7441 catccgggca gcttcgctat tcgggcctcc aggtcgtcgc agagacgctt ctcctcgccg
     7501 atcacgctcg gacgctccag gaagaagagg agcgtctcca gcaggcgctc cgcgaggggt
     7561 tcgccgctca agcgctcacc ccgaactcgc gcagggcgtc gttgagggag gtcttttgat
     7621 ctgtggactc gcggcgctgg ccgatgatga gggcggtctg gatctggtag gagccggctg
     7681 ggaagtccct ggcgcgggtc ccggcgacga cgacggagcg ggccgggacg cggccccggt
     7741 ggaccacctc ctcggggccg gtcacgtcta tgatgcgggt cgagcccgtc aggactacgt
     7801 tcgccccgag gaccgcctcc tcctcgacgc gcacgccttc aaccacgata gctcgcgacc
     7861 cgataaacgc cccgtcctcg ataacgaccg gcatcgcgcc tggcggttcg aggacgccgc
     7921 cgatacccac gccgccggag aggtgcacgt tgcgcccgat ctgggcgccc gagcccaccg
     7981 tcgcccacgt gtccaccatc gtgccggtgc ccacgtaggc cccgatgttc acgtagcccg
     8041 gcatcactac gacgcccggc tcgacgaagg caccgtagcg gacggtccct ggcggcacca
     8101 cccggacccc ggcctcggcg aggttcttct tcgtcgggat cttatcgtgg tactcgaacg
     8161 gcccggcctc gatggtcttc atctccgcga ctccgaagta caggttgatc gccttcatca
     8221 cccaggcgtt cgactcccaa tcgccgttac gcttctcggc caccctcaac tcgccccgat
     8281 ccagggcggc tatcgtgtcg aagacggcgg agcggtgctc ctcgtctttc agcagtccac
     8341 ggtcctcgaa cgcggcctcg atcttctctc tcatcccggc ggtcctcctc tgatcgtcta
     8401 aacgtattcg actggcacgt gcgccagttt aacaggagga tggaacgtat ccgtggcatc
     8461 cgcgcgccgg agaaacggtg gctaggggca gctatactgg ccgcgtgtct tcccggaact
     8521 cgcgatccga acccggtgga gagaagagtc cgtgacctcg ctcgcgttgg cgctggtgct
     8581 cgcggcggcc gtcttccacg cgacctggaa cctcttcgcc aagcgtgtcg gcgacggcgg
     8641 ggctcccttc gtctggctct tcggcgcgct ctcggcgctc atctacgcgc cgctggcggc
     8701 ggtcgtggtc ttcgtcgaga gaccgcactt cgggccgatg gaactgctct tcatgggtgg
     8761 cagcggagtt ctgcacctgg ggtacttcgt gctgttgcag cggggctacg ccacaggaga
     8821 cctgtctctc gtctatcctc tcgctcgcgg tacggggccg ttgctggcga ctgcggcggc
     8881 catcgcgctc ctgggtgagc ggccgtccgc cctggcgctc tgcggcgtcg ccctgatcgc
     8941 ggtcggcgtc ttcctgctga ccagggagcc gggtagcgtc cggggcgcgg ggttcggacg
     9001 ggcggcgatc tacgggctgc tcaccggggg gttcattgcc gcctatacgc tgtgggacaa
     9061 gcacgccgtg agcgcgctcc tgatcccgcc tctcttgcag agctgggcca cgaccgttgt
     9121 gacgacggcc ctgatctcac cgctggcgtt tcgccacagg ggaaaggtcc gggcgttgtg
     9181 gcgaacgcac aagttcgagg cgatcggggt cggagtgctc tcgccgctct cgtacatcct
     9241 ggtgctaacg gcactcgtct ttactcccgt gagctacgtg gccccggcgc gtgagatctc
     9301 catcctcatc ggcaccgcga tgggggcgcg cctgctcgcc gagggcgaca ccgcccggcg
     9361 cctagtcgcg gcgagcgcga tggtcgctgg cgtcgtggcc ctggcgctgg gctaaatgtg
     9421 tctaacaaaa ccgcggtgcg agttggcgcc gaagcagtgt ggttccgcgg aggatcggag
     9481 ccgtgacgaa gcggtgaact cttcagaaga gctcttcagc cagcgctaca atgatgcccc
     9541 ctggaccccg catgtagcag agccggtaca agttctcgta ttgggccact tcgccgacga
     9601 gttcggcgcc gtgagcgtgc atgcgagcga cagtgtcgtc gacgctttcg actgtaaaca
     9661 tgacgctccg gaggcccagc gtgttcggcg gtgcgatcgc cggttcgatc tcgaccagtt
     9721 ttggattgtg aaacttcgtc aactcaagcc gcccgtgccc atctggggtc cgcatcataa
     9781 cgatgtcgac ttggacactt tcgagcctgt tgacgcggtc tacccactct ccctctaccg
     9841 ccgcctcacc ctccagctcc aaaccgagct cggaaaagaa agcgatagcg gctgcaaggt
     9901 cgtcgacgac gacgctgacg tggtccagtc tcttgatcgc catgtcctca gtataggact
     9961 ggcggagcgc gacggttcag gtcgactgtt cttaccgcta ccgtctaacc cagccgacca
    10021 cctggcttcc cagaggtggg tcccttaacg attgcttacg ctacgtgggt taggcgggtt
    10081 ttggtaggtg tactgagtgc caacaggggc acgcgcgcgc taagattatg cttctagacg
    10141 gagtccgagc actacgaggt cccgctcggc gccatcgagt tcggcgactt gcgggaagcg
    10201 agcccagcgc tcgaacccga accgctcgaa gagcttgagg ctgggcccgt tgtggccgaa
    10261 agcgccggcg gtcagggtct ttataccgag ttccggggcg cgacggatga cctcttcgag
    10321 caggcgcctg ccgattcctc tgccccggaa cccgtccctg acgtagatgc cgatctcggc
    10381 ggtctggtgg tacgcggggc ggccgtcgta gaagtcgctc aggcttatcc atcccaccac
    10441 ctccccgcct tcctccgcga ccaggatcgg acgcctcgcc gggtcgtgcg cccggaacca
    10501 cggcagacgt tgctcgacgg agaccggctc ggtgtccgcg ctgaccatcc ggctcgggat
    10561 cgtggagttg tagatctcca agattaccgg caggtctgcc tctatcgcgt ggcgtatctc
    10621 catactcgcc cccggtagtc tctcggcttg ctagactcct acccgctccc aaagctctat
    10681 cgcccgccgg cagtccgcaa gctccggcac cagggcgacc ctcacgtacc ccgcgccgcc
    10741 cggcccgaac gacctccccg gcgccaccac gatgccctcc tccatcagcc gcagggcgta
    10801 agcctcgtca tctccggcga acgcctccgg caccgtcacc cagaggtaga agctcgcgtc
    10861 cgtgggcagg tactcaaggc ccgagcgctc gaagaagtct atgaaaagcc tccgcttgtc
    10921 ggcgaagatg cgacgccgat cctcgacgtg ctcgtcgtcg ctccaggcgg cggtcgcggc
    10981 gtcctgcacg aaggcctggg tcgccacgcc gattgacggc ctcatcttct tcagagctgc
    11041 gattagctcc gggtctccgg ccatcatcgc cgagcggtag ccggtcatgc cgctgcgctt
    11101 ggagagagag cagaaggcga gggtccgctc ccgcgtcact tcgaggatgg agggcggagg
    11161 cgtgtcggag tagatgtcgt tgtagcactc gtcggagaag agcaggatgt cgtgctcgcg
    11221 gcagaactcc gccacaccct ccaggtactc gtaggtcgcg gtcgcgccgg tcgggttgtg
    11281 ggggtagttg atccagagta tccgcgtcct ggcggggtcc acgctctcca ccggcagcag
    11341 gaagccgtcc tcgcgccgca accggaccgg cagaggctcg ccgccggcga actgggtccc
    11401 tcgctcgtag actggatatc cgggcgtgcc gtaggcggct ccacgccgct cgtgcgagtg
    11461 gtgcagtaag gccagcgggg cgtggaagat cgcctctttc gagcccgtcg ccggtaagac
    11521 ctcggtctcc gggtcgagcg cgacgccgtg gcgacggcgc atccagccgg cgaacgcttc
    11581 gcgcaactcg cgcttgccgg gcgaggacgg atactggctc acctccggga cgccctggat
    11641 gagggcctga cggatctttg cgtccgtcgg ctcgcgcggg tcgccggtgc cgaagtcgta
    11701 gaggtccgtg ccgcgctcct gcaactcccg gcggcgctcg tcgaggcgca ggagggggta
    11761 cgcgccctgc cactccagta ccgggttgag cagcaggggc ggaggaccgc tctttagtgg
    11821 ctggtggtct gtgctggtgg tcgcgccggg ttctgacacg gggtatattc tccccagggc
    11881 tctcttgcag gagccagaaa tatagcacgc cccggagcgt gaaggcggag ccagaggagg
    11941 taggttttgg acgactttca ctacgagggg ggcgacctct actgcgagga cgtgccgctc
    12001 gccgagatag cgcgggcggc gggaaccccc acctacgtct acagccacgc cgccctggag
    12061 agggcctacc gggatctgga cgacgctttt tcgggccttg accacctcgt ctgctacgcg
    12121 gtaaaggcca acgggaacct ggcggtcctt cgggcgctgg cctctttcgg ggctggggcg
    12181 gacatcgtct ccggcggaga gctttaccgg gtgatgaggg ccgggttcga cccgaagaaa
    12241 gtcgtcttcg ccggcgtggg caagaccgag caggagctga tggccgggct cggggagcgc
    12301 atcctgctct tcaacatcga gtcggcctcg gagcttgagc acctggagcg cctggcgtcc
    12361 cggcacggca agaaggcccg cgtctcgctg cgcgtcaacc cgaacgtgga cgccgagacg
    12421 cacgagcaca taaccacggg gcgggagcgg gacaagttcg gcatcccggt ggacgaggcg
    12481 ctcgcgctcg ccgagaggat cggggagttc cggtgcgtgg acctgatcgg ggtccaccag
    12541 catatcgggt cccagatcac caagatagac ccccacaccg aagccatcga gaggagcgcg
    12601 ggactcgtcg aggagctcaa gcaccgcggc ttcgacatca aatacttcaa catcggcggc
    12661 ggcctcggca tccgctacaa ggacgagaaa gtgccgaccg cgaaagacct ggtggatgcc
    12721 gtccggccca cccttgagtc gctcggggcg agcggcacga agatactctg cgagatgggc
    12781 cgctacatcg ccggcaacgc gggggctctc ctgacgcgtg tgatctaccg caagaagagc
    12841 ggcgagaaga gctttatcgt cgccgacgcg ggcatgaacg acctcatccg cccgagcctc
    12901 tacgacgccc accacgaggt gcgggccgcg cgcgaggacg ggtctctggc gaacgcggat
    12961 ctcgtcggtc ccgtctgcga gtccggcgac tacctggccc gcgaccgcga gctgcccgac
    13021 gccgccgagg gcgaactgct cgccgtcatg agcgccgggg cctacggctt ctcgatggcc
    13081 tccaactaca actcccgccc gcgccccgcc gaggtcatgg tccggggaga tagcttcgcc
    13141 gtcgtgcgcg agcgcgagca caacgccgac ctcataaagg gagagctggt cccggctttc
    13201 ttgtaacggg agccgacggg cgcatgctcc gccagagttc tactcgttaa gtcgtgcgtc
    13261 gctacggcgt agcggaccag tggggctact ctacgtcctc tggcgctgat tgacgaggag
    13321 gggggagagg cctggacggc ggcgggccag cgcggagtca atggagagca ccttcgcgga
    13381 ccggctgagg aggattagca gcgagatcag ggcgaccacc acgtttatcg tgaggaactc
    13441 gggcgccccg aggccggtgc cgagccggac gcctccctgg gagagcatga tgttgatgct
    13501 catggctatg ctgccgatgg ccgccaggtt ggtgagcaga cccaggataa gggctatccc
    13561 cagcgatagc tccacgacgc gggcgacctg agcgaagagc tctgcgttgg gcacgacggt
    13621 gcccttcatg aacccctgga agaacggcgg ggcctgagag acgaacccgc ccgccttgag
    13681 gctctgggcg aactgctgcg ggaaatccgg gttcaggagc ttctcgatag cgccgttgag
    13741 ccagacggcc ccgatcagga tccgcaccca cgccatagct ctcatccgcg aagcacctcc
    13801 ctgatagccc gagccctcta ctttttagta tacgcgcgcc cggaacgaaa ggatggacaa
    13861 accgcggcgc aattctctgc gtaagtagca gatgtacggg aaagggcgga agcccacaga
    13921 tgaaggagga tgccttgggc gaggaaagag tacccaggag ggagttcatc cgctatggtg
    13981 cggcagtcgg gctcagtgta gccggcgcct ccgtgctcgc ttcctgcggc gggggcggag
    14041 attccgcgca gagcgaagag agcacgaaga agaagtcttc ctccagcggc ggcactacgg
    14101 accccacggt gggctcgggg gaacctatag ttttggcggc cttcctgccg ccgggcttcg
    14161 ccttcccatt caccatagcc gagtcgggca agcccgcgct cctggtacac ctcaaggacg
    14221 atagctgggc ggcctacgag gctgtatgta cgcacaaggg atgcgagata ggctaccagg
    14281 tggatcagcc cctgctgcgc tgcccgtgcc acggctcggg cttcgatccg gccgacggcg
    14341 cggtcgcgaa cggacccgcc cagaagccgc tgcagaaggt aaaggtcaag ctcaagggtg
    14401 acaacgttgt gcgcgcgtag cgccgtttgt cgaacatctt gaaacgccgg gagccgggag
    14461 cgggctgtta cgccatggct cccggcccgc gttcatgcgc tggatcccct gggttacggt
    14521 gctgcgggcg ctccgctcgg gatcgtctcg ggtgaccatt cgtccggcgg aaagtcgggc
    14581 acgggcgaca cgccgtcccg cacggccgcc caaagggcct ctccgtcacg gtgaccttcg
    14641 atcacccgct ccagatcggt ctcgcgagac cattgctcgc gtggtaccgg cgctccgtcg
    14701 aagccgtggg cgaacccctc ggcgtaggga cggccaaact cccggcgcgc ggcctcgtag
    14761 tgttgctcac cggcgagacg ggggatcacg ccagcctcca cgataacgcc aatgccgcac
    14821 gccgcctcga gcgccgcccg gcttgttcct tccggacggg taaagcgggc gaacgatccc
    14881 cgctcgggcc gcgcgcccgt gatctgatac gccccgacgc ccgtctcgac agctatgcga
    14941 ccctggccgc tcttctccgc ggacatcagc gcgcccctcc gaagaccgcg ctaagtggta
    15001 tgttgccgta gccggggacg gacagcccca ccgaccgcgc cccgtcttcc gtcacaccgt
    15061 ctccgttgcg tgggaagatc cgggcttcga cggagcgcca tccgtaaccg ggagccttga
    15121 tcttgaggct cgtctcgacc gggaggtctc ccttctcgat gcccgaaagg gcctcgaccg
    15181 ggaacacagt tctgacaggt actccgccgt agccggctgc ctcgacccgc agggatactt
    15241 ccctggtttc tacagaccat ctctcggtcc ctagcgtttc gttcctgaga gcttgcgcca
    15301 tcttgcctga acctccgttt ggatttcgga gtccggatct acatcgctat cggcggcgcc
    15361 gaaccggacc ttacgctctc aaaggtaacg tcgcggtatg aacttgtcca agacaatttg
    15421 cagcagtatt aagacctcag agatctgaaa ctccgggaac gccatccggg atgtccgcga
    15481 catccgcgac ggccctcacg acctgcagga aggctctcac ggtcggggtc tcgcccccgc
    15541 gccgccaggc gacggccgtg tcgggggcgg gaggatgccc gcgcagggaa cggtagacga
    15601 cgccgtggta gcgcaggtgg cgcatcgagg cggggacgag tgcgaccccc atcccggcag
    15661 cgatcaggct cacctgggtg ggtagctcgg tagcctcctg caccacccgc gggctgaagc
    15721 cggcgtcctg gcaggtgttg ataacggcgt cgtggagcac ggcgcccctg cgccggggga
    15781 agatcacgaa cggctcctcc gacagttcgc gggggtcgac gaccctgcgg cgggcgagcg
    15841 gatggtctgc cgggagcgcg gccacgaaag gttcccggcg cacgaccgcc acctccaggt
    15901 ccgggttttc ggtcgaggtg cgcaggagta tggcgatctg gatctcgtct tcgcccagag
    15961 cccgcaccag ctccgagctc agcaactcgc ctcgcaactc gatcccgagg tccggcagcc
    16021 gctcccgcga cgccttgagc agcaggggca atacgccata tgcggccgag cccgcgaaac
    16081 ccaggaccag ccgtcccaac tcaccgcgtc ccgcgcgtct ggcagcctcc tccgcccgct
    16141 cattgtaggt gagcacgcgc cgggcctccc gaaggtacac ctcgcccgcg gacgtcagct
    16201 ccacccggcg cttggtgcgt tccagtagct cgacgccgag ctcgcgctct agctggatga
    16261 tctgctggct caatggagac tgagatacgc gtaaccgggc cgccgcccgg ccgaagtgaa
    16321 gctcctcggc gacagccacg aagtaacgca ggtgccgcaa ctctaccatc ggctgctcct
    16381 cccgtccctg gtcgcgcgcc ggtcatttac tttcacgcat cggtgtggag gcttgtgaag
    16441 atctccgcca ttcatcacgg tcgctccttg acactcgccg atctcgccgt tgtagctcct
    16501 gaatatccga atcttctccg gcgtgtggcg tcgtgccggc tactcgtcag agacatttgc
    16561 gtagcgatgc tccgccgcgt ccctgtcggc gtagaaccat agtacatgtc cgtgcgtata
    16621 tagaccaaga tccctgaaag cctactgtgg aggaggaagt tttggagagg atttcgcgcg
    16681 aaaggttcat tcgtgttggc gccgcgctgg gcgtgagcgc cgccagcgcg tcggtgcttg
    16741 cggcctgtgg cggaggagac tccggctctt ccggcggcgg gaactcggga ggcagcaccg
    16801 ggagcagcgg ctcctcgggc gaggcctcca ctgttaagtc gaaggacggt tcgggcgaga
    16861 cgaccaagac ggagaatgcc tctggcaaag ccagcggcgg gtccagcagc ggagcgatag
    16921 cttcggagtc cgaggtggct actggtagcg cgaagagctt caagaactcg gggcagccgg
    16981 ctgtcctggt ccatctggat agcggcgatt tcgtggctta ttcggccgtg tgcacgcatc
    17041 agggatgcac ggtggcctac aagggcggtc agctagcctg cccgtgccac ggctccatct
    17101 tcgacccggc aaatggggga tcggtggtta acggacccgc gcagcagcct ctgccggaga
    17161 taccggtcaa ggtggagggc gggcaggtcg tcaaggcgta gcagtcagcc gctctttaca
    17221 agaacccggc cgccggatgg atcaccgctg gcggctaaag tagaatgccc tcatggactc
    17281 cagtgaggag cggatcgggc taaacggcgc ggcggtggtt ctgggcgtgg cgccggagga
    17341 agtccggcgc ttcgtgaatg atggacgcct gcgcgccgag cggacggaga acggcggtta
    17401 cagcctcaat ttcgctgaag tccacgctct ggcgagagag ctggaggtcg agagcgcgga
    17461 ggaagaggtc tttgagggag aggtgatcgg ggaggagagc gatcctcggt cccgcctccc
    17521 cgccgtcccg ttcgagcgct acgaacgtct cctccagcag ggccaggatt acaagacccg
    17581 cctcgaagaa cgcaaccgcc agatggaaga catccgcgtc gagcgcgacg ccgcccgtgt
    17641 cgagatagac cgcctctggt ccgagataga acgcctcaac ctaatcgagt tccagcgcga
    17701 gcttcaggag aagacgatct ccactctcga agaagagaag aagaagctcg aagccgaacg
    17761 cgaacgcctg atcaacgacc gcctggaact cgtcgaggag aagtccggcc tcgtcgagga
    17821 acgtatccgc ctcgaagccg aactccaggc cctcaagaac cgcggcttct ggtcccgcct
    17881 cttcggcggc tgataaaccc cgtaccgggc ttgtacataa aaaggaaagc tcccggctct
    17941 cgtgagagaa gccgggagct cgaatacgct atctgtactg gactactgct gagttacgtc
    18001 ctttccgggg ccaccgcgaa gaacgtccct gccgaggcca ccttgcagcg tgtcgttacc
    18061 gctgctgcca tagagcttgt cgttgccacc attaccctga agcgtatcgt cgttttcctg
    18121 accgtagagg gtatcggcac cgtcctgtcc ctgtatcgcg tccttgcctt ctccaccgta
    18181 cactacgtct ttgccgctac cggcctgaat ggtgtcgttg ccagcgccgc ccttgataag
    18241 gtcgttaccg ccgagaccct tgatgatatc gttgccgccg aggccgcaga tcacgtcgcg
    18301 cttggaggtg cccttgagga tgttgtccct gtcgttgccc ttaatggtgc aggcgttggg
    18361 gtcaggcgtg gggttcttct cggagttgtc gctcctagag gagcagcctg gatccctaag
    18421 gtccaccttg ctgtcggcgt cgttgtcgcg gccgtcgttg caggctggct tggggtcagg
    18481 cgtcgggtcg ggcggaggag gcggaatctc cgtctcaacc tttatgtcgt ccaccgaagc
    18541 ccggaaaaag aactgactct cgaccccggc aaagcgcaag cgcacagtct ggccagcgta
    18601 aggactgaga tcgaaggtca tcggcgttgg ctcgagggtg ttgggatctc ccgcgttggt
    18661 gcggtagacc gagtccagga tatccgcggg atccaccgag aaggggtcgg cggacggctt
    18721 gaggatgtcc acccggtatt gctgattagc tccggtactg tagtctagcg tgtcaggggt
    18781 ggagaagccc tgggcctcgt tctggtagta gaggataaac gagagcgtgt gctccgcgtt
    18841 ctcctctaga gcgatgtcct ggtagaggac gtgtgaccct gggccagtct ggtccgtggt
    18901 ggcggcgaag tttccctgcg gcggagccgt gatctgccgc ccagactggg gcgacgcggt
    18961 gccactgtac gcataccagt cgccgttgcc accgttctgg ttcacgaccg tccatcccgt
    19021 gagcgtgccg ctctcgaagt caccgttcgc taccgtggcg gcgtgagccg ggctggcgaa
    19081 cgccagagcc gccatcaaga gcgtcaggac taggggcacc agcgccttca ccgaccatac
    19141 tctcaataaa ccattactca acatgttttg ctttcttcgc ggtcctcatc agcataggtc
    19201 tctccttgtc acctttcgcc tcgcggcgaa tttgcctttt gtatccgtca atacgattat
    19261 cggacgcatc ggagaaaaac tgtaaccata cgatatcctt gagttgacga ctttgtgcat
    19321 ccaacagccg tctggcgtgc ggcgggacta cttccgggtc aggcagggcg cttggctgga
    19381 ggcgacgcgg gggcaggtgt ccgttaccat ggtattctgg aagatggcgg agaggggcct
    19441 ggatcttcag ggcaccgatt ccgcggagac tcgccgcaac atggaggaga tgcgcgactt
    19501 ctacgctcac tgggaacgcg agttacccgc catcttcgag aggtgggaga aggagaagca
    19561 atgagcctgg ctatagagac caggaacctg accaagacct acgggcgcaa ggcgaggggg
    19621 atctcggagg tgaacctcca ggtggcgccg ggcgaggtct tcggtttcct cgggccgaac
    19681 ggagcgggca agacaacgac catacggacg ctgctcgggt tcctgcggcc tacgggtggg
    19741 gaggccagcg tcttcgggct agacatccgc aaggagagcg tggggatacg cgctcgtgta
    19801 ggcaacctgc ccggcgagtt cgcgctggag gacaggatga ccggcgaggg tctgctcagg
    19861 ttcttcgccc ggttgcgcgg cgtgaaggat ttgggttacg cgcacgagtt ggccgagagg
    19921 ctcggagcgg agttgcgccg tccgatgcgg cggctctcgc gggggaacaa gcagaagatc
    19981 gggctcgtgc aggccatgtt ccatcgtccg cctcttctca tactggacga accgacgagc
    20041 gggctcgacc ctctggtgca ggaggagttc ctggacatca tcgaggaagt cagggatgag
    20101 gggaggacgg tcttcttctc gtcgcacaac ctggccgagg tcgagcgggt gtgcgaccgg
    20161 gtcgggatca tccgcaatgg caggctcgcg gcggtggaga ccaccgagac cctcgtggac
    20221 aagtccttcc gccacgtcgt cctgaccttc gcggagagcg tggacccgaa accattcgag
    20281 gcgctgccgg gcgtgaagaa cgtaaagttc gatggcgcga aggtgtcctt cacgctctat
    20341 gacaacctgg acgagatggt gaagctggcg gcgaggcacc ggctggtgaa cctggagtac
    20401 gagcggccga gcctggagga agtcttcctc acctactatg gcaaccacag gaacggagaa
    20461 ggggcatgag cgctgcgcag gcggtcaaga agagagcggg aaggcagagg gttgagcgcc
    20521 gcgcgccgcg ggacgcgctg ctggctctga tctcacacac cgtccggatg cagctccggg
    20581 gggtgcttat ctggggtggc atcctgggcc tctacagcgc cgcgatcgtg gccacctacc
    20641 cgaccttcgg agacatgcag gggttcgatc agttcgtgca ggcgtatccc aagggattga
    20701 gagaggcgtt caacattacg gacatgagca ccatcgaggg tttcctggac acggagatct
    20761 tcagcttcat ggcgccgctg gccctggctt tcttcccaat cctggcggcg tcgagcgcga
    20821 tcgcgggcgc cgaggagcgc ggcactatag atgtcttgct tggcaacccg atacagcgct
    20881 ggcagctcgt ggccggcagc ttcgtcgcga cggccgtatc gctgctgggc atcctcgcgg
    20941 tcgcgggact attcgcctgg atcacggcgg ccctggtgga cgtggacctc tccgtcgaga
    21001 atgcggtgaa tggatacctg aacctgtggc cgatatgcct gttcttcggt ggcctcgcgc
    21061 tgctgtgctc ggcgatcttc caccggcggt cgctcgcaat agctgttccg ggcgttgtgc
    21121 tcctcgggat gtacctcatg gacgtgctcg gaaaagtctc cgaagacctt gaggacttac
    21181 gaccatactc cctcttctac tattatggat ctgcgatcag agacggcata gactgggtga
    21241 acttcggtgg gataaccctc gtcacgctgg cgctgatcgt tctggcgatg atcgctttcc
    21301 ggcggcgcga catctacacc tagccgttcg tccgggttca ctgaacggtg gcggcggtta
    21361 gaataaccgt tgcctatgac ggagcgcacc aacagagatg accggagcgc cgccttcccg
    21421 acgctggcgc gcggacagat cgaggtcctc aaacgcttcg gggaggtgcg cgagatcgag
    21481 gaggacgagg tcctctttaa ggaaggcgac gcctcctacg acttcatcgt gatcctggag
    21541 gggctcgtgg agatccggga gaacttccgc ggcgagtcgc gcaccgtcgc ccggcacggg
    21601 gcggggcgct tcctgggcga gatgaacctg ttcaccggcc aggccgtcta cctgacggcg
    21661 ctcgtgacgg agccgggcga ggccatcttc atcccgccgg agcgtctcaa ggagatcgtc
    21721 gccgaagagc cgggcctgag cgagttgatc ctccgcgcct tcctgatgcg gcgtgtcatc
    21781 ctctcggggc tcgggacggg gctgaaggtc gtgggctccc gctactcgga ggacaccttg
    21841 cggctgcggg agttcgcggt gcgcaacggc atcccgcacg cctggatcga ccttgaggaa
    21901 gacgagggtt cggaggcgct gctgcgggag ttcggcgtcg agccctcaga gacgccggtc
    21961 gtgatctggc agggcaccga cgtgctcagg aacccgacga acgcggaact ctcccgcgcc
    22021 atcggcctcg gcgcggacgt aacgccggaa gacacctacg acctcgtcgt gatcggggcc
    22081 ggccctgcgg ggctcgcggc ctcggtctac ggagcctccg aggggctctc cacaatcgcc
    22141 ctggacgccg tggcccccgg tgggcaggcg gggacttctt ccaggatcga gaactacctc
    22201 gggttcccgg cggggctctc gggttcggag ctggcgagcc gggccatgat ccaggccgac
    22261 aagttcggcg cgcgctttcc ggggccgcgc gaggtggtgg ggctgcgccg cgagaacgga
    22321 ctctaccgcg tcgccctctc ggacggcgag gaggttgcgg gccgcagcgt gatcatcgcc
    22381 accggcgccc gctaccgcag gctggacgcg ccgcgcctgg aagacttcga gggcgtaagc
    22441 gtctactacg ccgccaccga ggtcgaggcc cagatgtgcg cgggctctga ggtgatcgtc
    22501 gtgggcggcg gcaactcggc ggggcaggcc gccgtgttca tggcgggacg cacgaggaag
    22561 gtctacgtcc taatccgcgg cggcgacctg agccggggca tgtcccgcta cctggtgaac
    22621 cgcgtggagg ggaccccgaa catcgaggtc ctgacccgca ccgaggtgcg cgagctgctg
    22681 ggggatgaac atctggaggg cgtcatcgtg gaggacaaca gttccgggga gcggcgcacg
    22741 ctcggagcgc gggcggtctt cgtgttcatc ggggccgatg cgcacacgga atggctggag
    22801 gacgtgctgg agctggacgg gcgcggcttc gtggtcacgg gaccgtcgct ggagtcttcc
    22861 gacggcgtcg cgtggccgct cgcgcgggac ccgttcttgc tggagacgag cctgccgggg
    22921 gtgttcgccg ccggggacgc gcgcagcggc tccgtcaagc gggttgcatc tgccgtgggc
    22981 gagggctcga tggcggtgcg cttcgtccat cagtatctct tggagagccg cgtggaggcg
    23041 gtctccggct gaacttactg tgctagagtt ccgccctgta tggagagtag ggatctagac
    23101 cggctgctgt tgaggacgcg gggtctcgcc gtactgcgcg gggccatcga ctcgcccgtg
    23161 gcgcgggact tcctggcgct gctgcggctt ctgagcgtgg agcggccgga agccggggag
    23221 cttgcggagg tcttcgggcg tctctgggag ggactggcga tggagaacga gcgtctgctc
    23281 cccgacgcct ggcagtcgca tctcgtgggg cgcatcctgg acgatgagaa ctccttcagc
    23341 cgcggcgcgg agttgggcgg cgcggctgtc gcgatccggg atcaggcgcg gagggacctc
    23401 cgcacgttgc ggacgctctt tgacctcgac gccccgacgc tgctcggcat gatcgaggcc
    23461 gccgtccccg gcctcaccag catctgggcc ccctggaccg ccccggagcc ggactctgag
    23521 tccgcccgcc ataccatcgc ccgcaagctc gccgccgccg gagactggga ctcgtgcgcg
    23581 gagcttctcg aaggccactt cgcccgtcac ggcgcggggc tcttcggcgg caaccgggct
    23641 ttccgctggt gggagggaag cctccacccc gttccgcgcc ccgacccggt gcgtctctcc
    23701 ggtctcgtcg cctacgaccg cgagcggcgg ccgctgatag agaacaccga gcgcttcctc
    23761 tccgggctcc cggcccatca cgccctgctc tacggcctgc ccggcaccgg caagtcctcg
    23821 accgtcaagg ctctcgccaa cgaatacgcg gacgacgggc tgcggatcgt cgaggtggcg
    23881 aaggaggacc tgtcggagct gccgcgcgtg ctcgaaacgc tgcgcgggcg cgggccgcgc
    23941 ttcatcctct ttgtcgacga cctctcgttc gaggagcacg aggtcgagta caagtccctc
    24001 aaggcgctgc tcgaagggag cgtcgaggag ccgccggaga acgtccggct ctacgccacc
    24061 tccaaccgcc gcaacatcat ccgggagagc ttctccgacc gggaggacgg cttcgccggc
    24121 gacgatgtac acgcccgcga caccatgcaa gagaagctct cgctctccgc gcgcttcggg
    24181 ttgcgtgtta cctttccctc tccagaccag aaccgctacc tggagatagt cagcggccta
    24241 gcccgcgaga ggggcgtcga gatcccggag ggcgagcttc gggagcgcgc tctgctgtgg
    24301 gaccgctggc acgccggacg ctccggacgc accgcgagac agttcgtgga cgagatccag
    24361 gcggagacat ccaccagggt ctagcccatc cgtcgtatcc ctctaagacc ttttatcctc
    24421 ctcatatatc gcgtttgcca gctacgttag cccatttgga ctagataaag ttcactgatt
    24481 tacgcgatgg cgtaagggga tccgcgaggc atgatacccg catagcctcc acgctaacgc
    24541 ggatgaccca ttgccgaaga ggccgcaagc aaacaacgaa gccgaaaggg gcctcatgta
    24601 cacggagtcg cggggatcat ataagttctt catgttgctg gtagcgatac ttctaacttt
    24661 ggcgtcggtt gcgacgtttg cggggagggc gaacgccctc gttcaggaca gctccccgac
    24721 agtagagtcc ccggacaagc cgaacgccga ggacgacgcc tactcagtgg atgaggacaa
    24781 cacgctcgtg gtagcggagc ccggcgtcct cggcaacgac acggacccca acggtagacc
    24841 gctgcacgcc gagctcaaga ccggccccgc gaacggtacg ctcgacttga acgaggatgg
    24901 ctccttctcc tacacgcccg ccgccgactt ctccggcacg gacagcttcg tatacgagtc
    24961 ctgtaacaac atcgaaccgg agatctgcga cgaggggacg gttaccatca cggtcaaccc
    25021 ggtcaacgac ccgcccttcg cggacgatga tagcgcgacg accgacgagg acaccgccgt
    25081 cgtcatagac gtcaaggacg gcgactctcc cggcccggcc gacgagtccg ggcagagcct
    25141 gacggtcgat tccgttaccg acccggcgca cggcgcggcg gagatcatca cgaccggcgg
    25201 cgacgccggc aaggtcctct atacccccga cccgaactac agcgggtccg acgccttcga
    25261 ctacaccgtc tgtgacgatg gttctcccat caagtgcgat gtcggcacgg taaccgtcac
    25321 cgtcaaccag gttgacgacc tgccggagat aaacctggat gaggagagcg cccgcgtggt
    25381 gaacgagggc gactccagga cctacagcta cagcgtggag gacgtggacg cccccagcca
    25441 aatctccgag agctgcggcg ccaacgccac ctacaccgac actccggatg cgaacagctt
    25501 cgagtgcttc ttcccggatg gcccgaagac cactcaggtc tccgctctgg ttaacgtgcc
    25561 acaggagctc tcccaggcca gcgatacccg agtctcctcc ctcgaagacc tcggcgcccg
    25621 gatcacggtc cagatcaaaa acgtcgctcc ccgcctctcc ctgaccggtt cttctaaggc
    25681 caagaaggga cagaccctgc ccttccggtt caccatcacc gatcccggca ggaacgacac
    25741 catgagcttc ggagccggct acccgctttg cggccagggc gccaggctcg cctcccggcc
    25801 cctgatcggc ggcggcctgc tgaagtgctc ttacctgaaa tccggctccc ggccggtcct
    25861 ctccatgcag gtgaaggact cggacggcgc gatgtcaaac agggtcaacc acatggtctc
    25921 ggtcgccgcc gcgaagccgg ctccgaagcc gcaccatgac aaaccgcatc acgacaagcc
    25981 gcaccacccg aagccgcatc atgacaagcc gcgcccgaac tgcaaggata tacccagcaa
    26041 gccgggtcac gtggggagca cgcacggcgg agacccctcc caggcgggcg atgcccgggg
    26101 ctcgtgcagc gggcatgcca cagccggctg aacgggatgt ctcgcgcata gataaagcga
    26161 taggatagac acgacgaagc cccggtccgc acctcccgga ccggggctct aatttgcggt
    26221 gggcatcccg gctagagctt ctcggcgcgg gccagtatct tctggaggtc tctctggagc
    26281 gcggcggctt cttccttcgg ggagatgctg ccggtgagcg aggcgtggaa gcgtcccgat
    26341 atcgccaggg acatgtcata gtagtatggt gaggtcggcc gagagcggac gttctttatg
    26401 gcctccttgc ccagcgccgc aacgggcacc tcctttagta tctcggagtc gtcgtagaga
    26461 gcttttaggg tagggaggta gccgcctttg agggcgcgtt ctttctgttg ctcggagccg
    26521 ctcaggtact cgatgaaagc ccaggccgcg tccatcttgt gcgtggcggc attgatcatc
    26581 aggttccagc cgccgagccc gctggaggac ctctcgccac tccgggccgt cgggagcggc
    26641 gccagaccta tctgctccgg tttcaccttc gagagcgaag ggttggacga gagcgcgtat
    26701 acgaacggcc agttccgcat gaagaccgcg tcgcccgcga gaaagaccgt gtaggcttcg
    26761 agctccttga agctcgatac cgcctccggc gacgtcccgc tctcgaccat gccgcgcgcg
    26821 gcccggagcc cgtccacggc ccgcggctcg ccgatgacga cccggtcttt cggtcccagc
    26881 acgtcgccgc ccgcgttcca gacgaactcc agcgcgttca ctaccccgcc ctcgtagtcc
    26941 gcgccctgga aggcgtagcc gtacttgata ttctggtctc tctggacctt ctgcgagatc
    27001 tccatgagct tgtcccaggt cctgggggct tcggagaagc cgctcttctc cagcaggtcc
    27061 ttgcggtagt agagcatccc cgcgtccgta aaccacggca cgccgtagat gccgccatcg
    27121 gatgtgttgg aggcgatggc gccggggagg tggcggctct ggaggtcggg agagaagcgg
    27181 ttggagaggt cggagatcca acctttcgca gcgaactgag cgggccagat gacgtcgccg
    27241 cttattacgt ccgcgcgcgt ctcaccggcc tggaagccgg tgcgcagcat ctcaaagtac
    27301 tggcccgtgt cggccggggc ttgatgcagc acgaccttta tctcgccctg gtggcgccgg
    27361 ttgaagcggt cgatctgctc ctgccagatc tgagctccgt cgggaccgtg agtgaagatg
    27421 agcttcgccg ggccttcgcg ctcgcctttc gagcatcccg cggctcccag cagagatacc
    27481 cccgcgattc ccacgccgcc tagcttcagg aactcccggc gattgatgac ctttggtccg
    27541 gacgccgggg tcgtcatttc gggagcgtga agtagaaagt cgcgccgacg ccgggcttgc
    27601 cctcggccca cacccggccg ccgtggcgcc ggatcacgcg ctgcaccgtc gccagtccta
    27661 ttccgttccc ctcgaactca tcggccggat gcaggcgctg gaacgccccg aagagcttgc
    27721 ccgcgtaggc catgtcgaaa ccggccccat tgtcgcgcac gtagtagaca ggttcttctc
    27781 catcgcttcc gggttcggag ccgaactcca cctgagctac atcctctccc gcggtgaact
    27841 tccaggcgtt gccgagcaag ttttcgagcg ccacccgcag tagcctggtg tccccctcgg
    27901 cccgcaggcc atcctccacg gctagcttta cgcggcggtc cgggtcggcg gcctgtagct
    27961 cggcggctat ctctcgcgcc agggcgctca ggtccacggt ctccctgaag agcggcttgc
    28021 gcgttacgcg cgagaggtcg agcaggtcgt ctatgaggtg gctcatgtgc cggctcgcgg
    28081 ccttaacgcg ctctaggtgt ccgcggccct cctcgtcgag acggtccgcg tagtcttcga
    28141 ggattatctg cgagaagccc tcgatgctcc tgagcggggt ccgcaggtcg tgagagaccg
    28201 agtaggagaa cgcctgaagc tccgcgttgg tcgccgccag ctcgcggttg gccgaggtga
    28261 gctcttgatt ggtggcttcc agctcccggt tcgcggattc tagatctcgg ccgacccgct
    28321 cggcggcggc gcggttgacc gagagcatcc acgtaacccc gaagagcatc aggcttatag
    28381 cgccgccgct caggagtacg aagagcggga ggttggtccg ccagccgctc tcgaagccgg
    28441 gaagcgagct gaagtacagg gtccagatgc gccccgcgac ctccatcgtg gtggtgtcct
    28501 cgaagggcgg agagtaggag ctgtcggcgg cgtgcagggc cccgtcgtca tcgtgcagca
    28561 gccccgagga ggtcagctcc gcgccgtcga agacctccag gtcaacgccg ggtgatgtct
    28621 gatctccgaa gatacccgcg agcaactcgt ccgctccaaa gacgccgacg atgtaaccct
    28681 gcaaggactg acggcgcgcg tcggggttat cgtgaggcgc gccatttttg tacaccggcg
    28741 tgtagactac gaagcccggg cctccgccgg agcctttctt gctcggaagg tctattttgc
    28801 ccgaggcgcg gggcaggccc gtgtctcgcg cctgctccat cgccgcccgg ctcactcgtt
    28861 cggcgtagag gtcccggccg aagagagcgc ggttcgctcc ctcgaacggc tccacgtagg
    28921 taatggggaa gtactcgtag cgctcgcccg cgggccgcaa ctcgtagtcc gggaagccct
    28981 cgccgcgcag ccgggacacg tgtttatctc tcccctccag cgtgacgcgc ggggcgtagc
    29041 ccatggcccg gatgccggga tagcggcgct ggaggtcgga ggccgccacg taccggctcc
    29101 actcctcgcg ggtaacggag ttgctggcgg cgaagagtcc ccggccttcc agcatggcgt
    29161 ccacgtaagt gtccatgcgc cggtccagcg cgcgctgaac tccgctggtc gtctcgtcaa
    29221 agcgcgcgtt ctcccgcgcc tcgacgttct gctttacgta gagagcggcg aggacggtga
    29281 gcaacagagc gactaccagt acgccatagg ccgcggcggt gcgtctcagg tggtcccgga
    29341 cggcctgccg gatctctccg gttcctttga tcctggacgg caactcatca cgctccgact
    29401 ggcgggttct cgttgagcac cagccagtag gacttgagct gttcgatggc cgcggtgaac
    29461 tgcacgaagt tcaccggctt tcggacatag ctgttggcgc ccagaccgta gccttccagc
    29521 atgtcctgcc gctccgtcga agaggtcagg atcacgacgg ggacgagccg ggtgcggtcg
    29581 tcggagcgca tccgccgcag cacctccaga ccgtccacct ttgggagctt caagtccagc
    29641 aggacgagtt gcggaatgac ctccggatcg gtgtgggatc ctgtggcgaa gaggtactcc
    29701 agggcctcga ctccgtcgtg ggctaccacc accctgttca tgacgttgtt cttcttcagc
    29761 gcccggagcg tgagcagctc atcatcgggg ttgtcttcta ccagcaatat gaccttgtta
    29821 ctcatgtaaa cacccccgga ttatgccaga tgctacattg cgtaggggct cggcgcaagg
    29881 tctcgctgtt cgctccggag cgtcttttga gcggggttcg caaatccggt cttttctagg
    29941 ctacccggcg aggtccgccg tctatcctgt cggcgatgaa cgggtaacgg tagtcctcgc
    30001 cctggctgac cctgtatgcc gcgtagcagc cgtggacgag cgggacgagc gcgatcaggg
    30061 gcagcaggaa gaacatgaag atcccgacga tgacgaggct caggatcaga ctcaggatag
    30121 tgtaggcgat gagtaggacg acccatgcta gctgatacca gagcgcctgc agggcatgga
    30181 aacgcaccct ctctgagcgg tccctgtaca ccagccagat gatgagagcg ccgagcggca
    30241 tcacgccgat cagggccgcc agcacgctca ggtgcgagat gatggaccac gtctgctcgt
    30301 cctgcgggga catggtagtg ggatatgctt gccgctcggt ttcgttatga ggttcttgca
    30361 tagccgcttc tcctaagatt gccaggttac tttaaccgct cacatcatat tcccctggtg
    30421 ggtcgagcgc atcactcaaa tgagtgattt tctatgagtc gccatccgag tattctctgc
    30481 tggcaaaaga gaagggccgg cgatctagga gggggaaatt ggacgccggc ccggtaatgt
    30541 tcaaagtatc taccctggaa gtgtgtcccg gcatcactca aatgagtgat tttttgcgat
    30601 ttgcgaaaat tttttctgtt cgggttgcga gacgggcgcg cggatatttg ccaccggtcc
    30661 ggtacaagaa tagaattgga aggagtgaat ggagcgaggg agatctcttt gcctatcccg
    30721 aaatgggtag agcttgtccg gtatcgggtc gcgaagtgtg gccgcgccac gattattttt
    30781 gcgccggggc cgcgagagag agaggagtcc gggtgggcaa tccgctgaga gtcctggtgg
    30841 tggaggactc ggaggacgat accctgcttc tcctgcatga gctccggcgc ggaggatacc
    30901 agccgttcca ccagcgggtc gacaccgagc aggccatgaa agccgctctc cgcgagtggg
    30961 agtgggacct ggtcatctcg gatcactcca tgccgaactt cagctcgtcg gcggcgctgg
    31021 acctcttgcg gagcgagggc tacatggacc tgccgttcat catagtctcc ggtcagatag
    31081 gcgaggactc ggcggtcctg gcgatgaagt ccggggcgca ggactacctg atgaaggaca
    31141 acctccgccg cctcaccagc gccatcgaac gcgagcttcg ggaggcggag gtgcgccggg
    31201 agcgacgcct ggcggaggag gcccagaggc gggccgagga gaagtaccgg ggcatcttcg
    31261 agaattccgt cgagggcatc ttccagacca cggtggacgg tgcgttcctc atcgctaacc
    31321 cggcgctggc ccgcatgttc ggctacgaga ccgccgagga attgctgcgc agcgtctcca
    31381 gtatcgggga gcagctatat gtggacccgg agcgccgggc ggagttcgac cggctcgtgc
    31441 agcgggacgg attcgtgcgg gggttcgaga tccagatgta ccgcgccgac gggagtgtga
    31501 tgtgggcttc cgtaagcgcg cgcgccgtgc gcgacgagga gactggagag ttcgccggtt
    31561 tcgaggggac ccttgaagat ataaccgccc gcaagaacgc cgaggaggcg ctgcgcgaga
    31621 tccgggagtt cgagcggcgg cgtattgcgc gcgagctaca cgacgtggtg ctccaggacc
    31681 tgacctactc cctccagtcc atgcagcttt cgagggcggt cgccgcaacc gccgggtctg
    31741 tcggcgggcc cggccggggg tccggcagtg aggcggacga ggagcgggac cggcagatag
    31801 aagcgttgcg ccgggcggtc aaggggctgc gcgacgcgat ctacgacctg cggctggagg
    31861 gtgtccggga gcagccgctg gtgcggtcgc tggaagcgct cgtcgagctc aaccgccaga
    31921 tggttccaga ccgtgagata gagctggcgg tagacgaggg gttcccggac tccatagccg
    31981 gcgtctccgg ggtcgagatc acgcgcatcg cccaggaggc gctcgccaac atccggcgcc
    32041 actccggggc gcggcgagtg aaggtccatc tcagttcttc cggcgaagag gtgtgcgtga
    32101 gcatcgagga cgacgggcgg ggtttcgcgc ccggaacctc tcccggcatg ggcatgaccg
    32161 ggatgcggga gcgcgcgctc gccctcggcg gcacgctgga cgttagcggc gagcccggag
    32221 ccggaacacg ggtgtgcttc cgggccgatc tctcgaccct catggaggcg cccgtggacg
    32281 cggctggtgg taagaggcag ccgggagttt agtctcgcaa gctgctatat ttcttgctgg
    32341 taaccccggg ctgcgaaagt agtaatgaaa ggcagagatg catcgaatct tgcttgtcga
    32401 agatcacgca tccttccggc agacccttgc cctgatgttc gaccgggaga cggagttcga
    32461 ggtggccgcg caggccggtt cgctcggaga ggctcggcag gtggctgtgg agttcggaga
    32521 cgcgatggat ctcggggtaa tagacctgac tctcccggac ggcgagggga tagagttgat
    32581 ccaggagatc cgggaggtca gcccgcactt ctctgccctg atcctcacgg cgagcctgga
    32641 ccaggcggac cacgcccgcg ccatcgaggc gggcgccgcc ggtgtcctcc acaagtccgc
    32701 cgacgtggac gagatcgtcg gcgcggtaag gaccctcgcg gagggcaaga ccctgatctc
    32761 gccggaggag atcgtgactc tattgcgcct ggccgggtcc aaccgcgagc aggagcgcga
    32821 ggcgcgggcc ggcatagagc gcctgacccc ccgcgagctt caggtgctcc aggctctcgc
    32881 cgaaggcctc tcgaataagg agatcgccga gcgcctgcac gtcagcgtgg ataccgaacg
    32941 cacccacatg atgaatatac tgaccaagct gggcgtccac tcgcgcctgc aagccctcct
    33001 cttcgccgtc aagtacgata tcgtcgagtt gacttagagt cacgagtaga gccgcgagct
    33061 agagcagcaa ctctccctcg cagacatact ccgccggccc ggtcatgtag acgggctcgt
    33121 cctcgccggc ccactctatc tccacgatgc cgccgtcgag gtgtataagc gccgggctct
    33181 cggcgaggcc ccgagagacc gccgctaccg ccgtcgcgca ggagccggag cccgacgcga
    33241 gcgtctcacc cgcgccgcgc tcccagatcc tcatccgaac gtcgcgccgt ccccgcgcga
    33301 acgcgaactc cacgtttgtc ttctccggga aggttgggtc gttctcgata agcggcccga
    33361 catcgcgcaa ctccagcgcg tccaccgccg ccgcgctctc caggaacgcg accgcgtgcg
    33421 ggttgcccat cgagacgcgc agtaaggcga agccgccgac ctctacctct gtgccgagca
    33481 ctggaggacc catgttcacc cgtgaggagc cgtcttcccg cagcaccacc ttcttgacgc
    33541 cggccccggt acctatagtg agggcgtcgc cctccaccat ctcgaagtcc cgcgcgtagc
    33601 gggcgaggca gcggataccg ttaccgcaca tctccgagac cgagccgtcg gagttgaggt
    33661 agaccatctc gatatccgcg acctccgagg gggtggggac gagaatcccg tccgctccga
    33721 cgccgaagtg ccgatcgcag acgcgccggg ccagatccgg caggtccacg ccctcgacct
    33781 cgccgggatc gaagatgatg aagtcgttgc cgacgccgtg catcttggtg aatcgcatag
    33841 accgctaagg ttgcttcccg atctccaggt caacttcagc ctgccgtcat ggcgcgccac
    33901 gacgtccaag cctaggtcag tgtacacgga ccgatagctc ccggcacgct cgcgctttca
    33961 ctcccggcgc tgctccacat ctccgccgcc gtgcgcttcc tgagtatctc gggaaccacc
    34021 aactgcgcgg tttgtgcgga actttccgct ggtgctcttc atacctctcg ctcgcctgac
    34081 tttgcgctcg aacgaactgc gaggtcaacc gagacgcggc tattgctgcc agcgcctcct
    34141 tacatcgtcg gcgaaggccg tgtactcgcg aaccggcagg acttccgcca tcgtgaggta
    34201 atgggccatt ggaaggccgg ccatgaggat ctgatctagc tcgtcgtggg actcgacatc
    34261 cagaacgccc agcacgcgcc gctggccgga gaccttgtag agcccaacga tttttccggc
    34321 ctctatggcc ccgattgccg cgtttgcctc ttcctcccag gcctcccaca gctcatccag
    34381 cgacatatcc ttgggatcaa cacggacatc aacgaagacc agcatgttcg gctctccttt
    34441 ctcggacttt ccccaccgca tcttatacac cagattcgag agccctggca agagaacaca
    34501 gtcaggagga ccgcagcgtc gccatcgcaa gcgtggccga agtcaggcgc tgtcggatga
    34561 tctcttgcta tctgaccttc ggcgagtacg actggttcac cctcatcgaa gcagatcgcg
    34621 ccggcgcatg ttggtgaacc gcatgatagg cgtattttgg ctccgccgtt cgggtaagac
    34681 caggtcacgt tgtcggtgtc gctagtatgt acgagagaac aggccgtgta ggaaggtgga
    34741 gacgatggaa gatcagagag aagtgtgcct cgaagaagag gtctggagac tgcggcgagc
    34801 ggggaacaaa cccgaggaga tctcggcccg gatgggcgtg gacgtctcct gggtcgagca
    34861 ggtactggcg atgctcccgg atgaggaata tcccggcgag agcgcgaacc aggaacggtg
    34921 aggggtgcga gggggcgttc cggggtagat tgaggatatg ccgaacgaga acccgcacag
    34981 aagcgtgcgc cggaggcggc gcacggagga cgagcggcta ctctcggctt cggggctgga
    35041 cctcgccgcc ttcacggaga ccgatacctg gcgggtgttc aggatcatgg gcgagttcgt
    35101 ccagggtttc gaggagctgg cggatctcgg tccggcggtg acgatcttcg gctccgcgcg
    35161 ggtgagcgcc ggggacgcga tgtacgagac ggcgaccgag atgggacgcc tcctgggcga
    35221 ggcggggttc acgatcatta ccggcggggg accgggagtc atggaagccg gcaaccgcgg
    35281 cgcgcgacag gccggcgctc cctcggtcgg actcaatatc gagttgccgt tcgagcagca
    35341 cgtcaacccg tacgtggaca tcgaggtgga cttccggtac ttcttcgtcc gcaagatgat
    35401 gctcgtcaag tactcgcagg ccttcgtcat cttccccggc ggtttcggta ctctggacga
    35461 gctgttcgag gcgcttaccc ttatacagac cggcaaggtc aggaacttcc ccgtcgtcct
    35521 cttcggctcg gactactggc ggggacttct cgactggttg aacaccacga tggtgacgga
    35581 aggcaagatc tcctccgccg acctggggct cctggctgtc accgactcgc ccgccgaggc
    35641 gcgggacctg atactggatt ctgtcagaga gcagcaatgg agcgccgaac acgaggaggg
    35701 cgcgcgcgag gcgacccgcg aggcgttcgg cgaaaaaccg ggaagttagt ccccggtctc
    35761 agggcctcca atccccagca cgtcgcccat cgagtacagg cccggctccg ctccggccac
    35821 gaatttcgcc gcccgcagcg cgccatcggc gaaggtgcgc cgcgaaaggg cgcggtggat
    35881 gacctcgacc tcctcgccgc cggagtagaa gatgagccgg tgctctccga cgaccgcgcc
    35941 gccgcgcagg gcgtggatcc cgatctcacc ctccttccgc ggcgcgaggc cctcccgccc
    36001 gtaagtggct acctcgtcga gatcctgccc ccggccctcg gcggcggccc gtgccaggaa
    36061 gagcgccgtc ccggaaggcg catccttctt gttgcggtgg tgcgcctcca cgacctcgat
    36121 gtcgtagccg cttccgagct ttgcggacag ttcccgcact acctcgcgca gcaggttcac
    36181 cccgacgctc atgttcgggg cgagcattac cggcgcgctc ttcgccgcat ccgcaacctg
    36241 cgacatttga tcctccgaga gccccgtcgt cccgatgacg tggggcttgc cgtaggacag
    36301 gtgcgcgacg gtcgcctcgg gggtggtgaa ctcgaccagg acctcggcat tctccggcgg
    36361 ctcctcgctg gcagttactc ccaccttacc cgccccgcac aactcgccga ggtcggcgcc
    36421 cagctccggc gctccgggct cgacggttcc tcccgccagc tcgatgccgt ccgtctccag
    36481 ggcggcgcgg cacagctcgc gccccatgcg cccggcggtg ccaattatgg ccacgcgagt
    36541 caagggccta cacttcctcg ggcgtgggga ggaggtgacc gtggcgctca agggcttctt
    36601 cgaggccgcg cagcttgtcg ccggacatcg gcatcagggg gaggcgcatc tcgtcggagc
    36661 agagtccgag gagggaggcg gcggtcttta ccgggatggg gttcgtctcg acgaagagcg
    36721 ccttgaagag tgggaggagc tggtagtgga tctcgcgtcc gcgcgcgaag tcgccttcca
    36781 gcagcgcgtt gaccatgtcc gcatagatgc cgggggcgat gttggaggta acggctatga
    36841 cgccggagcc gcccagggcc atcagcggga gcgtcatcgg gtcgtcgccg gagagcaggt
    36901 cgaactcctc gccgcagagg cgctttatct cgcccgcaat ccccgtcgag agcgtcgcct
    36961 ccttgacccc gacgacgttc ggtacctcct cgtagagccg cgctatcgtc tccggctcga
    37021 tggcgacgct ggtgcgtccg gggatgttgt agaggatgag cggcaggtcc gtattctccg
    37081 cgatggcggc gaagtgccgg tagaggccct cctgggtcgg cttgttgtag tagggggcca
    37141 cctgcaacga gccgtccgcc ccgacatcct cggccatctt ggtgtacttg atcgcgctgt
    37201 cggtgttgtt cgagccggtc ccggcgatca ccggcacccg cccgttggcg acccgcacga
    37261 cggtctctat gaccagccgg tcctcagact cgctgagggt cggggactct ccggtggtgc
    37321 cgcaggggac gaggccgtgg atgccgtgct gtatctggaa ctcgaccagg ttctccaggg
    37381 cctcgacatc cacctcgccg ttcctgaacg gtgtaacgag ggccgtgaac gcgccgctaa
    37441 acatctctct cctccagttc gaagtccttg tggagacgcc ttaccgcctc ctctactctc
    37501 tccgccggca acacgcacgt tacctgtatc tccgaggtcg agaccattcg tatggggatg
    37561 cccgcctcgc ctaaagaact gaacatcctc gccgcgtagc cgggacggtt cagcatcccg
    37621 gtcccgacga ccgagacctt gcccaggtct tcctcgccct cgaccgcgcc gcccaaagag
    37681 tccgcgacct ccgcggcgag ttttcgcgcc tcggcgaagt tgcctcggga cacggtgaag
    37741 gccacgtcgg agaggccgcc ttccaggccg ctctccacga tcacgtcaac cgagatgccg
    37801 cgctccgcga gcggtgcgaa gacgcggctc atggtaccgg ggccggtcct tatcctgttc
    37861 agggttatgc gcgagacgtc caggtcgtgg acgatgccgg cgacggtctc ccgggtctcc
    37921 aggtgttcca agtgcttacc tcctactatc gccgtgcccg gctcctcgtc gaaggagctg
    37981 cggacgtgga tctcgacccc gtagagcgcc cctagctcga ccgcccgcgg gtgcatgacc
    38041 ttcgccccgc gccaggacat ctcggccatc tcctccgatg agatgacggg gattcgccgc
    38101 gcctcgggga ctatgcgagg gtccgccgtg tagatgccgt ccacgtcggt gtagatctcg
    38161 caccgctccg cctccagcgc cgccgcgagt gcgaccgccg tcgtatccga gccgccgcgg
    38221 cccagcgtca tcacgtcgcc cagggcgttc atcccctgga aaccggcgac gatgacggcc
    38281 tgtccctcgc cgatatactc tcggatgcgc ttcggctgga tctccgatat aacccccgag
    38341 ccgtagcggc ctgtgatcct gaaccccgcc tgcggccccg tgagcgagac ggccggcaca
    38401 ccgcggtcgt gcagcgccat cgccaggagc gccaccgact gctcctcgcc ggtggcaagg
    38461 agctgatcta tctcccgcgg cgacggctcc ctgctgacct ctcccgcgag cctcaagagc
    38521 cggtccgttg tcttgcccat cgcggagacg actacgacga tgtccaggcc cgactcccgc
    38581 gctcgtccga ccctcgcggc tacggccttt atgtgctccg ccgtggccac cgaactgcca
    38641 ccgtatttct gaacgacgat ccccaaagac ggcttcctcc cgctgttcct gctcccgtgt
    38701 agcctagcgt atcaggcgcg ttttcgcagc atggctctcg ccgggcgcac ccaagatgtt
    38761 gtgggtgatt ttcaggtgtt cgcaacatga agaactttgg tgacgggcga aaaaagatgc
    38821 gtgaaggtct tgcgcggcgg ctgacagtgg ttcagaatag cgaccgcgtt caaaatctca
    38881 tttctcgctg gaggggggct cttgcgattt acatctgtac gtcacgccgc gggcagcggc
    38941 ggttacggcc gaggactctt tctcggggtc gcggtatccg tggtgatgtt cgtggggata
    39001 atctcccatg cggcgcaggc ccagacgccg gagatagagg ccaagcaggc cgaggtaacg
    39061 caggcgcagg cccggataga cgcgaccctc gaagacctga acaaggccca ggccgcctac
    39121 gacgagtcgc aggacaagct ggcgaaggtc gagggtgaca tctccaagaa ccaggacgcg
    39181 ctgaagaaga cccagaagcg gctggatgac gcgaaggaga agctctccga ccgggcggtc
    39241 ggctcttaca agtccggggg agtcatgtac ctggatgtcc tgctctcggt gaagtccttc
    39301 gccgacctct ccaacaaggg ccagttcatc ctgcaggtgc tcaagcagga ccgggacgct
    39361 gtgatcaaga tcgcgaagat aaaggacgat ctcgccggtc agcagagagc cttgaaggag
    39421 aagaagcaga cgcaagctgg catctctgat cagatgcgga agcagcaggc cgccatagac
    39481 gaccggctcg ccgagcagcg ggcgacctac gcctccctca gcggcgaggt gcagcgcatg
    39541 atccaggagg agcaggcccg gcaggcgcgg gaggccgccg aacgtctcgc gaaggccgaa
    39601 gcccaggcga acgctaaaaa ggccgccgag caggaggccc aggccgagaa gcaggccgcc
    39661 gctcaggcga cccgtcagca gatggcccag gccaacgccg gcgagcaggc cagcgccgag
    39721 gccgagaagc aggccctcgc cgagaccacc gcccgccagc agcgccagca gctcgccgtc
    39781 caggctgacg aggccgacaa gagagcggag aaggcgaaag acaagaccga gaaacagcga
    39841 ctcgccgccg aggcggagca ggcgcgcaag aaagccgccc agcaggccgc cgccgagaag
    39901 gccgcccgcc agaagcagca gcaactcatc gagcaggccc agcaggctgc tcaggctgca
    39961 aagcaggagg caaaacaggc ggcggatcag agcgatcagc agagcgatca gagtgcggcc
    40021 gcggatcagt acgcgagtgg aggcgaatcc gctgctcagg atcagtatca ggactcttcg
    40081 tcgggagggg agacgaccga tgcctcctca gcggagccgg accccgcggc cacctcttcc
    40141 gatccgggcg cgcaggccat actcgacaac ccgaatgtca gcctcacctc gatggctcag
    40201 caggacctct ccgccggcat cgttgactcg cgggttctgg acgtggtggc tttcgcagcc
    40261 caggagcaca gcatcgcgat ctcggtcttc aagaccgggc atccctacgg cccgaccctc
    40321 gacgctatcg gcctcgcggg ctacccgaac tcccactact acggccgggc ggtagacatc
    40381 tatgaggtgg atggggtgcc ggtatcgagc ggcaaccagg cagcccacga cctggcccag
    40441 tccatcgaag accagttcgc gccggcggag cttggcagcc cctgggtctt cggacccgga
    40501 tctttcacca acgagatgca ccaggaccac atccacgtcg gctgggccta cagcgcggac
    40561 ggcgggttgt aacagccagc agattgacta atgaacggtg aagagcgccc cgtctgcaca
    40621 ggcggggcgc tcggacttga tgcccgccgc cgacaaaaag ctgacgggtt gaagctggtt
    40681 ggctgaccgg ttccctacgc caccggctgg ccgcccgtga tgtctatgac cgcgccgttg
    40741 acgaagctgg actcgtcaga ggcgaggaag acgaaggcgg gggagacctc taccggctga
    40801 ccgggacggc ccgtcggcga ctgctggccg aaagaggaga ccgtatctcc gggcatggag
    40861 gcgggtatga tcggggtcca caccgggccg ggcgcgacga cgttggcccg gagcccatac
    40921 tctacgacct cctgcgccag cccctcggtg aaagtcacta tcgcgccctt cgtggcggag
    40981 taggggagga gcggcggcga gggcttgtac gcctgcgcgg agcccacgtt gatgatggcg
    41041 ccgcccttcg gcatgtgcgg cagggcagcc ttcaccgtcc agaacatggc gtagatgttc
    41101 gtcttgaagg tgcggtccag caggtctgtc gagacttcgg agatgctccc gaccgtcatc
    41161 tgatgggcgg cgttattgac cagcacgtct atccgaccga actcctcgac ggccttctcg
    41221 atgaggctga cgcacgtcgc ctcctcgctg atgtctccgg ggaacctgac gcagcgcctg
    41281 ccggactcct cgacggcccg cgccgtctcc tctgcgtcgg actcctcttc ggggaggtag
    41341 gagatcaaga cgtccgcccc ctcacgggcg aaggcgaggg cgacggcccg tccgataccc
    41401 gagtcgccgc cggttatgac ggctttcttg ccttcgagcc gtccgctgcc ccggtacgtc
    41461 tcgtagccgt agtcgggctt cggttccatc tcggactcaa gaccggggtg ctggtctcgc
    41521 tggtcctgct ccgggaactc cggctgcggg tacttctctc tcgggtcctg ttgcccgtgc
    41581 tggtctgtca tcttcggtcc tttctctagt cgtggacacg cctatacccg cagttgcgga
    41641 actgttattc accgcccgtg catatttcgg atcatttcga gcgggcgcgg cgtctctttt
    41701 cggaatggtg aaaaatgggg agttacccgg cgacgagtcg tccggggaag gagacgttga
    41761 tgggagagta cgaggcagcg tttcagaacc agccggtgga catcagcggg caattcgccc
    41821 ggcaggagaa ccacttcttc ctcggtgaga aggtcgagag cttcgatcct ctgatggcgt
    41881 ccggagagat aatttggaag agtctctccc tgaagcagcg cgtctcgtac catcaactca
    41941 ccctccagtt cgaggactac aaggtgtggg aggacacgcc gccgggagag tatgaggacg
    42001 accaggtcct ccctttcgac atctccttcg tgagcccgag gaccgtccgg ctccgggtgg
    42061 cggcccggcg agagcctatc cgcgagaagg actcgctcat gctcgtcgga gaacccggct
    42121 ccgacggctc ctgggagatg agcgacgaag gagggcgggt aacctacacg gctcctcacg
    42181 gctccgtcgt ggtggagcgc gagccctttc acttcgagtt ccgcgatgcc tcgggcaggc
    42241 tcctgacccg caccaaccac ctctccgaca cccggagcgt ggtcaactcg atgccgacgc
    42301 cgttctcgtt cgtccgcaac gcctcgaacc tgcaccggca catcgcggcc agcttcaagc
    42361 tctctccggg cgagaagcta tttggcggcg gggagtcctt cacgcggctc gacaagcggg
    42421 gccagaaaat ggtcctctac acctacgacg cctacagcgc ccagaccccc tacatgtaca
    42481 agccggtgcc gttcttcatg agcgaccgcg gatacgggat gttcgtccac acgagcgcgc
    42541 cgctcacttt cgacctcgga ggttcgtatg acggggcgaa cgtgatctac ctgggcgacg
    42601 acttcctgga cctgttcgtc ttcatcggct cgccgaagga gatcctcacc gagtacaccg
    42661 ccctcaccgg ccgcgccccg accccgccgc tctggtcgtt cgggctctgg atgggccgcg
    42721 agtcctactc ctcagagcag gagacgaggg aggtggcgag gaagctgcgc gagcaccgca
    42781 tcccttcgga cgtgatccac ctggataccg actggaccga gaccccgcac cgctgcgact
    42841 acgagttctc cccttcccgc ttcccgaccg ccgaaaagat gctctccgac ctgagagaag
    42901 acggattccg cgtgagcctg tggcaattgc cctatctcaa cccgatgaac gaactgcacg
    42961 ccgaggcgat agagaaggat ctcgtggtcc tcagcgccaa cggtaaggag cccgtggacg
    43021 acgccgtgat agacctcggc aagccggagg ccgtggagtg gtatcagggc aagctgaaga
    43081 agctcctgga gaaaggagtc ggggtcttca cggcggactt cggcgaggcg gccccgctct
    43141 cgggcatcta cgccgcgcgg cacggaggat ttcacgagca taacctctac ccgctgcgct
    43201 acaacaaggc cgtcgccgag atcacggagg aagtaaacgg ctacggtgtg atcttcgccc
    43261 gcagcggctg ggccggctcc caacgttacc cgctccactg gggcggcgac gccgagaaca
    43321 ccgacacggc tttagccgca accctgcggg ccgggctctc tttagggctc tgcggcttct
    43381 cgttctggag ccacttcatc gggggcttcg cctaccagag cccggcggac ctgtacctgc
    43441 gatggctggc gttcgggatg ttctgctccc acagccgctg ccacggcgcg ccacccaggg
    43501 agccctggga atacggcaag gaattcatgg acggcttccg gcgcatagtc gagacgaaat
    43561 accggctcat gccctacgta tacgcccagg ccaggctctg ctccgagcgc gggcacccca
    43621 tgatccggcc gctcttcttc gagttcccgg aggaccgcac ctcttggctg atcgaggacg
    43681 agtacctctt cggcgaagac atcctcgtcg ccccgcggct ggaggacgcg cccggccggg
    43741 acgtgtacct gccgccgggg ttctggatcg actaccagac cggggaggtc tacgagggcg
    43801 cgaggtggca cgacatccgg gccggagatc tcccgattgt gctgctcgtc cgggacggcg
    43861 cggcgatacc gcacgcggga ctcgcccaga gtacggaatg gatcgactgg agcgagatag
    43921 agcttcgtgt ctacggcgcg gagacacgcg ctgccagggg cctcttctgc ctccctgaag
    43981 acggcgagtc gcacgtattg cgccttgcgc gcgagggtgg gagcttcacg ctggcagagg
    44041 atcccctgcc gcatagagtg cgctggagcg tgaacgttta cccatagacc ggcccggtac
    44101 gcgccacatt ctgcgatgtt tggctcccag gaatcacggg ggatatcccg gtagactcgg
    44161 gagagacaca agaccgacga aggagaggac atgagcgagg acgagcgtcc ggaggggatc
    44221 ttcgaaggct acgacaagaa cgacgaggta gagatctcct catatgccgc gctggtaggt
    44281 ctgttcaacc tgctcttcgc cttattcctg tttcttgccc ggcgtacagg gcggccgatc
    44341 ccggagcgga tcgagactcg ggacatcctg ctcttaggcg tggcgaccca caagttgagt
    44401 tggctgttgg ctagagagcc cataacgagc cccattcgcg ccccgttcac cgagctcgaa
    44461 gaggtcaaga gcccgacgaa ggcggatgag aagcctcggg gcaccggcct ccggcggtcg
    44521 gtcggcgagc ttctgacctg ccatttctgc ctcgggatgt gggtggcgtc gttcttctcc
    44581 tacgcgctgg tgctctttcc ggcggcaacc cggctcgtcg gggccatctt cgccatgctc
    44641 actgtctcgg atcacctcca ccagacctac aaggccctga tggaccggac gtaatatctc
    44701 cagcgcggac gaaccggcta tctctgctcc ctcctttggc ggggcgcaat ggtcggatac
    44761 cagcctcgag gcgtccgaac aacggttgcc gggtagaaag ctctccagcc ctgataatgg
    44821 gagagagctt gagcgagggc cacatccttt ttgtggacga ccggccgcag aacgcggctc
    44881 tcttctcgcg cgtactcaag atccacggta tcaccaacga agtgatccct gccggggacg
    44941 gcatcgaggc tctggaatac ctcttcggaa cgggacagca cgccggacgg ggcacgcgca
    45001 tgccgcgact gatcatcctg gacatgcgca tgccgcgtat gggcgggctg gagacgctgc
    45061 ggcgcatccg cgccgaccgg cgcaccgaga tcgtgccggt ggtggtgttt tcgtcctccg
    45121 acgacccgca ggatatgctc gaagcctatc gtctgggggc caacagctac gtggataagg
    45181 tgtcgctgaa cctgccgtat cccgagatcg ccggtatgat cgcccggtac tggctcctgg
    45241 tgaacgagcc tccgccgggg acgtagcctg cgctagcagg acgcgcgatc tacgcgagcc
    45301 gcacccggcg cagccgcagg tccgtcaggg cggcctttgc cgcggagtcg ccggggacac
    45361 cgtggaccgc gccgccgggg aaggtggccg cgcttccgat gtagaggccg cgcaggggcg
    45421 tgcggtaggg cgagagcgag ggcagtggac ggaagaagag ctggtccggg gcctggctcc
    45481 cggcgttcac gtcccctcca acgaggttgc agtttcggga ttccaggtcc gctggagtca
    45541 ggacgtgccg gccctggatg aggtcccgga agccgggagc gaaacgctct acctgagcct
    45601 cgatcctctc tacgtgacct tccagactcg ccgggtccgc gccggacggg acgtgcgtgt
    45661 aggcccagag ggtgtggcag ccctccgggg cgcgggaggg gtcggcgacc gttggctggg
    45721 cggcgagcag gaacggactc tccggcagcc cgccgagctt ctccgtagct atgccctcgg
    45781 atagctcccg cgtgccgccg ccgatgtgga gcgtgcccgc gccacgcgcc gtctcgtaag
    45841 accacggcgc cggacccgag agcgcccagt ccaacttcag ggagcccggc ccgtaacggt
    45901 agcgggccat ctttctcttg tacgagtccg gcagcgcgtg ggccgagatc ccgagcaggc
    45961 cctgcggagt tacgtcgcac acgactacac cagcctcgaa tatttctccg ccgacctcga
    46021 cgccgcgcat cctgccgttc tccgccacga tgcgctccac gggggcgttc acgcgcaact
    46081 cgccgctcaa ggagttcagg taactcacga gggcggccgt gatgcggccg gaaccgccct
    46141 cggcgaccgg ccatccgacg gcgtggccga gcagattgag atagaagccg aagaccgcgc
    46201 tcccgctagc gtccggcgcc acgtctccgt gcagcgccga gccgtagagc caggccttcg
    46261 ctccatcgcc ctcgaacagc tcgtccgcga gcgccgagac gggctggagg atcatgcgcg
    46321 ccgtctccag cgaccccgtg agtccgagac ccgccgccgt ccgcagcgac cccccaaccg
    46381 gcgggaaccc gccgagcatc gcccgccgga gcgcgggcca gaccttcagg tagggcgaga
    46441 tgaaacgccg ccacgcctcg ccgtcgcccg cggtcaggtc gtccagggaa gcggctgtac
    46501 ggtcgaggtc gcggtagaga gcggcgccgc ggctctcgtc gaggagatga accatcgcca
    46561 cgggcgggtg gacgaggcgc agaccatgct cgccgagcgg catgcgggcg aagacggggg
    46621 aggcggcggt tgccgggtag acaccggaga aggtgtcgtg aaggaagccg gggatggtga
    46681 gcgcttcggt cttcaccgcc ccgccaggcc ggtcctcggc ttcgaggacg agcacggagt
    46741 ggccggcttc ggcgagccgt atggcggcgg agagaccgtt gggacccgcg ccgacgacta
    46801 tggcgtcgta gcggccgctc atactctagc ttccgctggc tgtggcggcg cggcgcgctg
    46861 gcatggagat ctcatctagt gtccggccgc gagtctcctc gcctaagagc cacacgtcgg
    46921 cggctatgac gaacaggact acagcgaaca tcgagaatac ggcgagcgtc ccgaatccgg
    46981 gggcgccgag catcatcccg acgatgtagg gaccggcgat cccgccgatg cgtccgacgc
    47041 ccgcggccca gcccgcgcca gagccgcgca ccgccgtcgg gtagagttcc ggggtgtagc
    47101 cgtagatgac gccccacgcc ccgaggttga agaacgagac gagcgagccc cagagcacaa
    47161 gctccgcgcc ggaggtctcc gccccgaagc cccgcagacc gaagacgagg gtcgccgccg
    47221 cgcagcagag caggtagctc accagggtcg gcttgcggcc ccacttctcg acgagatatg
    47281 cggctgagaa gtaaccgggg atctgggcga gcgttatgag cagcacgtac tcaaaggact
    47341 tcactactcc gtagccggag gttgcgagta tctgcgggag ccaggtgaag atcccgtagt
    47401 acgagaacac catgccgaac cacagcaccc acaacatcgc cgtccggcgg gcgtaggacg
    47461 tggcccacaa cgctccgatc ccggtcttcc cggcttcctc gtcggtaacc tcccggtccg
    47521 gtccggtgtc ttcgatcccg agtcgccggg ccgcctcgcg cgcctcgccg tagcggcctt
    47581 ttcgcagcag gaacctgggt gattccggca gaccgcggcg caggacgagg acgtagagcg
    47641 ccggcagcga cccaaggacg aacgcgatcc tccagccgaa ctccgggatc acgaggtagc
    47701 ctatgagggc cgcgaggatc caaccgtacg cccagaagct ctccagcagc acgatcaggc
    47761 gcccccgatg gcgagctggg gccgtctcgg cgacgagcgt gctcgcgacc ggcaactcgc
    47821 caccgagccc gacccccgtg acgaaccgca ggacgagcag cgccccgaac ccccacgcga
    47881 gagccgtgag gcccgtggcg acggagtaga cgagcagcgt cgtcatgagg agcgtcttgc
    47941 gcccgtaacg gtcggagagc gcgccggaga cggccgcgcc gacgaacatg ccgaagagac
    48001 cggtactgat cgccagccca acctgcccct cgctcagacc ccactcctgc gccagcacga
    48061 ccatgacgaa cgacagcaac ccaatgtcca tcgcgtcgaa catccagcca aaccccgaga
    48121 gcagcgtaac cctccagtgc cccggattca gcggctggag atccaacctg ccctccaatc
    48181 ctcccactcc cacaccctgc atcccgaacg cctccttagt tcagccgcct ggatcagctg
    48241 ctcgtaacag aaacagaagt gcttgataca cgaatctgct acaaggtaag ctgtttgtaa
    48301 caaacctcta catgaagtat cacacaagct ataaagtccg gcaagctctg tgtctactcc
    48361 gggccgggtg cggaaggacg gccagattca gagggttcgg ctagaggagt gtggagctca
    48421 actccgccgt gccggagctt tatccagagt acgatgagca ggccgccgag cgccatcgct
    48481 gacaggccga tcaggcccgc ctcgggtccg aaggctccgc cggtcaggag gacggggccg
    48541 gattgtttta cggtcaggaa gctcgcgccg atggtgtcca ggccgctgac ggggaagccg
    48601 tagacggcgc cctggaagaa gttccaggtt atgtgcaggc cgatggggat ggcgagttct
    48661 ccggtcagga cgtagccgag cccgagcatc agaccggcga cggttatgtt aaggagtccg
    48721 atggccggga agttgggatt gcccgagtgc agccagccaa agaagatcga tgagagcgcc
    48781 caggcgagga gcaccgcgtt cttggcgccg agcataggat aattgagtcc ttcggccgcg
    48841 ttgcgaagct ggtagccgcg gaagatcaac tcctcgtaga ccccaactcc cgtgaatacg
    48901 acgaccggga cgagcacggc ctgcgcgaaa ggagttccag cgtagacgga ctcgaacgtt
    48961 gccgttatcg agatccatcc gagggcgagc tccgtcagga agatgaccgt catgagcgtc
    49021 gcacccagga gtagcccgaa gagtaggtcc agccaccacc tgacgttgat gtggaaaccg
    49081 aagtccgcga acgggcggcg gtcgaggagg cggcccgcca gccagacgct caggagcgcg
    49141 gctgagaggg aggcggcagc ggagatcagg ggcaccagcg gcgagtcgga gaggagtgag
    49201 gtgtctaccg ctccgctgga gaaagccctg ccgccggaga gtaacaacag cgacgccacc
    49261 gccagcaggt tgaccaggag cgcgatcagg accctgaacc ccaggtactg cagtagcatc
    49321 cgccagacag cgcgcgggcg cagcctgccg gtacgctcct ccaagagcag gctggccggt
    49381 cccttaactt caggcatgcg ttttcaggtt cggcatagag acaaactata acctcgcgag
    49441 catcattgct cgccgcgcgg cggggtggat ctagtacgct taccgggtgt ggccctgatc
    49501 tcgagaaagg aagacccatg agcgatcccg tccgtctaac cgtctactcg gactatctct
    49561 gaccctggtg ctttaacggg gccgtgcgtc tcgaaaagat agcgtccgag ttcgaggata
    49621 ccgccgaagt cgtgtggaag agcttcatgc tccggccctc gccgcatccc cggagcctgg
    49681 aggagcaccg cgagtacacc cggaactggc ggcgacccgc cgagcagccc gagtccggta
    49741 gtttcaccgt ctgggccacg gagaacgccc cgccatccca cagcgtcccc gcgctcgtcg
    49801 ccggaaaggc cgccgcgaca ttcggggagg aagcgttcca caggttccat ctggaggtca
    49861 tggaggcgta cttcaacgag aaccgcacca tctccgacac cgacgtgctc gtgaacgtag
    49921 ccggacggtc cggcatcccg gaggacgagt tccgcgagcg gctggttagc ggctggcagg
    49981 gtttcgagga cgaggtcttc gccgagcaca cggaggcgat cagcctcggc ataaacggca
    50041 taccggcggt cgtggtagac gacgcgctcc tgatccccgg cgccgtggac gcggaggttt
    50101 accggcgcgt gatccgggag cgcctggagc tacgggatgg atgatactcc cggcgcggtt
    50161 cttctaggac tcgtgtagcc ggatggtctc ccgaagcgct tcggcgtagc ggctggtcgt
    50221 ggtccggtag ttgctcacct ctcctgaggc gtgacggccc gagcgccccc acccgctgaa
    50281 gcggcctccg agcgcgaccg tgctgaagaa gacgccctga tagtagggga tgagttggtc
    50341 cagggtcatg gccggacggt ccgggcgcac ggagtcgtga gaatcgacgg cgttcacctg
    50401 cacctccggc ccctccaaac cgtgcttgtg cgtgttcttc aggcgcttca ggacggagag
    50461 gacttcctcc tcggcgggcc ggtcggcgaa gtccttgccc gcgtagaggt aggcgacctc
    50521 gccggtccgg tctatgagga ctgtggcggg taccgcgacg ccctccttcg cgtcccagag
    50581 gccgtagcgt ttcgccagct cgcccttcgg gtcggtcagg accgggaaag ggaggagaag
    50641 cttattcgac aacgccaggc tgtgctccgg cgggtcaacg cttatcgcgg cgacctcggt
    50701 gttgtagcgc gcgaaccgct cgaacccgcg ggccagactg gcgagctgtc cgttacagta
    50761 cgggcaccag tcgccccggt agaagacgag catcgccggt cccagctcca gttgcccgga
    50821 gagattccag gggtagcctg tctgatccgg cagctcgaag gtcggcgcct tctctcctac
    50881 gcttatctcg gccacacgcg cccctcctat aagagacctt acacctatca ttccgcatgt
    50941 tagcaggcaa cggcggctgg cggtgactga cggcgagcgc agggctcctg gaatgatcta
    51001 atatgtgctg gaagaagcga ggaacgatcc tggacttgca gttcacattc tacactctgg
    51061 cattgaccgc gggcgcggcg gttgcggcgg cgctcgcctt ctactcctgg cggcggcgcg
    51121 gctcttcggg ggcgcgggaa ctggcgctcc tgatggcggc ggtctccgtg tggagcctgt
    51181 gctacgggtt cgagttcctc tttaccgccc tcaatgccaa gcttttctgg gcgagcgtgc
    51241 ggtacgcggg cgtggtgacg gttccggtcg cctgggtcct gttcgcgctc cggtacacgg
    51301 gccgggagcg ctggatctcc ggccgcaacc tggcgctgct cgccgtcgtt ccggccgtta
    51361 cgctggcgct ggtgtggact aactgggcgc accatctgtt ctggctccac gccaccacgg
    51421 acaccacgga gacctttctc ctcgtggagc agggcgcgtg gttctgggtc ttcttcgcct
    51481 actcttacct gctcgtggcg ctcggcgcat tcttcctggt ctccatgttc gtccgctcgc
    51541 cgggcctgta cagggggcag ggcggcgcgc tgctcgtggg ggccctcgcg ccgtgggtcg
    51601 gcaacggtat gtatttcctg ggcttctggc cgctcccgaa cctcgacccg acccctctcg
    51661 cgttcctcgt gagcgggata gcggtctcct ggagtctctt ccgctaccgg ctgctggaca
    51721 tcgttccggt ggcccgcgat accgtcatag agaggatgaa cgacgccgtg atcgtcaccg
    51781 acacccgccg ccgcgtcgtg gacctcaacc cggcggcgcg gcgtctgctg ggcgcacagg
    51841 ggaccgggac cgtgggcagc ccactcgccc tgctggctcc cgacttcgac gctctcctgg
    51901 aagggtacgg cgaggcggat gaggctcaca aggaggtgcc gttcggcgat ggcgcggagg
    51961 ggcgcgagta cgagatgtcg ctctcctcgc tccgggatcg tagagggtat ctcacgggac
    52021 acctgctcgt cctgcgcgac gtcaccgagc gcaagctggc ggagagggaa ctgctgcgcc
    52081 agcgggcgga cctcgcgcgc accaacgccg agctcgaaca cttcgcctac ctcatcgccc
    52141 atgacctgcg cgcccccctc cgggccatca acggcttctc ccagattctc ctcgaagacc
    52201 acggcgacac gctcgacgcc ggaggccgcg actacctgac gcgagtggcc gacgccgccc
    52261 gtcacatggg acacatcata gacgagctcc tcgacctctc cagcctgacg cgcgcggaac
    52321 tgcgccgcga gacagtggac ctcagcgctc tcgtccgggg catctcggac gaactgcgac
    52381 agggggaccc cacccgtgca gttaccttcg atatacagcg agacctcacg gccgagtgcg
    52441 accggcggct gctcaggata gccctggcga acctcctgga taacgcctgg aagttcacct
    52501 cgcgccagcc cgaggcgagg atagagttcg ggaccgaaga gcgggatgga gccgtggtct
    52561 acttcgtgcg cgacaacggc gtcggcttcg acatggccca cgccaaaaaa ctcttcgggg
    52621 cctttcagcg cctgcactcc gcgagcgagt tcgagggcgc cggcataggt ctcgcggcgg
    52681 tagagcgcgt ggtcgagcgc cacggcgggc gcgtatgggc cgagagcgat atcggccggg
    52741 gcgccacctt ctacttcacg ctctgatccc gctccggcga cgccccggca aatttttccc
    52801 gagaccgttt cgcagcggcc cattattgta taaaatcccc gcctatggtc ctgatggaga
    52861 agccgggggg caggacgagg tcgcgcgcgc cgctgcggcg cctgcacgag cgtcagccag
    52921 agcagcagcc gcaatcacta ccgcggacgc ctttgcggac gcctccgcaa tcggcagagc
    52981 atacgcctca agcagtgttg tattcgttgc cgctgcgcgc gccgtcgcgc cagccgcagg
    53041 aaggtccggg gcgagccccc gaacgtactc ccggccggat acccgatcaa acacccaatc
    53101 ggaaaccgcg ccgccgcgct ttcggccgca agatcagccg taagaagatc tatcgccgcc
    53161 gtcgcagggc ggcgctcgcc atcatcctgg tcttcatgct ggccgtcgcc tggatgttat
    53221 ggcgctccgg ggaggacgcc actacggcgg gacccgctcc cattcctcag tccgaggtgc
    53281 ggaagtccga gacccggcag tccgaggccc cggcgaagtc cggcacggcg acggcggagc
    53341 cggcgcgggc ggcaggggac cccgacgtat ggggctcgaa aaagaaggac gcggagcaga
    53401 agctgcctcc gccgcccgcg gacaagacgc tctacctgac ggtacccaag atgggccgct
    53461 acgacgacgt ggtgcgaaac gacgactcgg agtgggcgct ggacaacgga gcgataaagc
    53521 tgcccacgag cgggttcccc tggcagccga gcgcaaacgt atacatcgcc ggacacgtcc
    53581 tggggtacgc gggcaccggg agctacatgc agttcgccaa ccttccgagc atggtgaagg
    53641 gcgatgagat cttcctgacc gacgccaacg gaaccaccta cgagtaccgc gtaaccgaga
    53701 tcctgaccgt caggcccgat gagaactggg tgacggcgcc agtagcgggc aaggatctgg
    53761 tctccctgca gacctgcatc aacccgccga actacgacca ccggctcatc gtgcgcggtg
    53821 agcgggtggg cgtgaagacc accgcataga gaaggtccga aagctcccgc gctaatccgc
    53881 agccgcaagt agcagccgcg agacgttctt ttccccgttc cggtagatcc ggtagaagcc
    53941 ccggtgcgta tcatcggatg caaggcattg aaagggcgag tgaggacggg ctccgtgatc
    54001 ctggcggcgg agtctccggc gaaggaggaa gtctctcgtg aagaaattaa gcatgtcgtt
    54061 actgctggcc gtggtcctgg ctctggccct cgcctcggtg gccttcgcgc agggaggaac
    54121 ccaccagaga gagttcaggg cgaaactcgc cacgctcaat caatcggggg cgacaggcgt
    54181 cgccgacctg acattgcgtg gcaggaggct tgaggcagag atcaagacca gaggcctgga
    54241 gccgaacctt caccacctcc agcacatcca cgggatggcg caggcgataa gcgagtgccc
    54301 gaccctggcc gccgacacgg acggcgacgg tctggtgagc gtcgccgagg gagcgccgtt
    54361 ctacggtccg atccaggtct ccttcaccga aacgggcgat acgagcgccg ccagcggcgg
    54421 agccctggac cgcttcccgc tcgcgaacgc caaggggcgc ctcgactatt cacgcacttt
    54481 ccgcatcccg gcggacgttg ccgcgaacgt gggagacttc gccatcgtcc agcacggcgt
    54541 ggacctgaac ggcaacggca gctacgacaa cgccatggaa gtatcgctac cggcgacctg
    54601 cggcaagatc gtgcggaatt agcgtctggc cgtctatcgc actgtatggc gccagccgga
    54661 gcctggtatt caggaaccgg ctggccgtcc caggatgacc tgtatcaagc cggggaggaa
    54721 gaggccaggg gagcgccggt aagagtcctg ttgctcatct acccggtgtc gcctggcgaa
    54781 gttgttcgca tgaacctccc gccgcagttc gccctgacga gcgtacagta gagatcggtt
    54841 caaatacacg ctgttcatct ttccctcttt ctcataaccg ttggcagtcc gtttccttac
    54901 atacgaacta tgacgccagg acgcgccggg cacatcacac aaatgcgtga tctttctcta
    54961 gcacttttgg atgagtttgt atgagatgtc tcgggacaaa caaaagggcc ggaaccttct
    55021 cggctccggc ccgcaggatc agtcggtggc cggttacttc aggaggtcct tggccgcgcc
    55081 cttcttctcc ttgagggtgc ccttggtctg atcgccgcgg ccctcggcct tcttgtcctt
    55141 gtcgccggtc aaggctccgc cggcttcttt cgcccggccc ttggccttgt ccacggcccc
    55201 ctccgccctg tcctggttgc tgctcttgtc cgacatactg tctctccttc cctggctctc
    55261 gcgttaccgg gccctctacc cgttcgccgc gagatgcaca cagagatgca cacaagaaga
    55321 gcgtagaatg cctcagcccg cgtgacgagc gccggagttc tcatccggca ctcactttcc
    55381 ctttccccac gctcgcgaga tcgctaccca ggctacccgc gccgaacggc atcataggcg
    55441 gcccgccggc tgctggaaat aggaaatatg acccaaacgc gccggcgtcc ttgtccgaac
    55501 ggctgtcctc tcgtccgccg ccctactcgc cggtgttcga ggcgcgcacg ctttcgaggg
    55561 ccgccgggtc ctccagggta gagaggtctc cggggtcgcg gctctcggcg agggcctgga
    55621 tgccgcgccg gagcatcttc ccggagcggg tcttgggcag ggcctcgacg aagtagaccg
    55681 ccgacgggcg ggctatggcc ccgatcttct tcgctactat ctccttgatg gactgctcca
    55741 gctccgagcg ccccgcctcg gactcgacct tatcaccgtc tttcggcacg gcgtaggcga
    55801 acacgacctg tcccttatcc tcgtccttca cgccgactac ggccacctcc gcgacatcat
    55861 cgtgggcgtt gatcgcctcc tctatctcgc gggtgccgag acgatgcccc gcgacgttga
    55921 tcacgtcgtc cgagcggccc atgatgtagt ggtacccttc ctcgtccttc atggcccagt
    55981 cgaaggtcga gtagagctgc tccttgaagt ccgagaagta ggtctggacg aagcgctcgt
    56041 cgtcgcccca gaccgtgctc atgcagccgg gaggcagcgg aggcgctatc gccaggacgc
    56101 cgcgctcgcc ggggcccgcc tcctcgccgg tgtcctcccg gatcaggcgc gtgtcgtacc
    56161 cgtagcacgg gaagcccgcc gaacccgcct tgaccggcgt atcttcgagc cccggcagca
    56221 tgctgctgag gatgggccag ccggtctccg tctgccagta gttgtcccgc acggaggtcc
    56281 ccagggtctc cgaggtccac tgcgaggtcg gctcgtcgag cggctccccg gccaggaaga
    56341 gtttgcgcag cgtgctcgtg tcgtgcctgc gcatgtactc ggggtcctgc ttcctcagag
    56401 cccggatggc cgtcggcgag gtgaacatgg tggctacgcc gtaccgttcc accagctccc
    56461 accagatgcc ggggtccgg
//
LOCUS       DASSMB010000285         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1250898, whole genome shotgun sequence.
ACCESSION   DASSMB010000285 DASSMB010000000
VERSION     DASSMB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250898"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFJ72_16340"
     CDS             <1..410
                     /locus_tag="VFJ72_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481075.1"
                     /translation="TDEVRAEAEGAVERLISLSRSGQEARRDMLDWLGTEFGIEKPGQ
                     KLQDFAALDADAFVEEVRKRRPKSEGRLTPADLRDLRSGYAEIATPVRERRAEAAGLE
                     RRLSDVVNQAYGLTPEEIDLLWSSAPPRMPKFL"
     gene            434..571
                     /locus_tag="VFJ72_16345"
     CDS             434..571
                     /locus_tag="VFJ72_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481076.1"
                     /translation="MKTTGYFENVILQVKRPYLEREWCERVRRNPESTAVEEDTGRIP
                     H"
     gene            703..951
                     /locus_tag="VFJ72_16350"
     CDS             703..951
                     /locus_tag="VFJ72_16350"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HET7481077.1"
                     /translation="MKLNYYPETDSLYIHLSEKPGADAVEIAEHVVIDVDERGIPVGI
                     DIDANASKIVDLSRLDLSGISLGDLAFTSEPKAREAAS"
     gene            972..1181
                     /locus_tag="VFJ72_16355"
     CDS             972..1181
                     /locus_tag="VFJ72_16355"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7481078.1"
                     /translation="MRRAQLKLAQLDQVGSVEELRKPPGNKLHKLGGDREGQWAIRIN
                     DQYRICFRFDNEAGDAYDVEATDYH"
     gene            1192..1536
                     /locus_tag="VFJ72_16360"
     CDS             1192..1536
                     /locus_tag="VFJ72_16360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET7481079.1"
                     /translation="MGDWTEEIEREERIHPPVHPGEMLREEWLGPLNMNANQLAKALG
                     VDRQNIYDIVNEKRGVSAEMALRLARWSGMRPEFWLGLQEHYELELARMESGEKIERE
                     VQPLTTATAEPQ"
     gene            1579..1833
                     /locus_tag="VFJ72_16365"
     CDS             1579..1833
                     /locus_tag="VFJ72_16365"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HET7481080.1"
                     /translation="MKVYYSPETDSLNIKLRTGEAGVVGEIVGEDLNENVVLHRDDAG
                     TLYEIEVVGSASKVFDLSRLEVEGLPVGVSPAIRQESKAF"
     gene            complement(1959..2360)
                     /locus_tag="VFJ72_16370"
     CDS             complement(1959..2360)
                     /locus_tag="VFJ72_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481081.1"
                     /translation="MKKTMALVLVVALMVAMFAGIAEAKGRPAGKGKPADKGNPVVTY
                     VFKGDVASVQDGSVTVDVQRANKFAQSYVGQQVEISVSDSTRIVKDDAPATLTDLVRG
                     DKVVVQSRAPRLGAESFTASGIVAESAAAQN"
     gene            2573..>2980
                     /locus_tag="VFJ72_16375"
     CDS             2573..>2980
                     /locus_tag="VFJ72_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HET7481082.1"
                     /translation="MSIEDYRSFYRRSIDDPEEFWAEQARIIDWHRDFERVLDFSNPP
                     FARWFVGGETNLCHNAVDRHLEERGDQNALIYVSTETDERREYTYRDLHAEVNAFAAT
                     LRELGVGRGDRVIIYMPMMAEAVFAMLACVRLGA"
BASE COUNT          630 a          880 c          971 g          499 t
ORIGIN      
        1 caaccgacga ggtgcgggcg gaggcggagg gagcagtgga gcggttgatc tcgctgtcca
       61 ggtccggcca ggaggcgcgg cgggacatgc tcgactggct ggggaccgag ttcggcatcg
      121 agaagcccgg ccagaagctc caggacttcg ccgccctcga cgccgatgcc ttcgtggagg
      181 aagtccgcaa gcgccgccca aagagcgagg gccgcctcac ccccgccgat ctcagagacc
      241 tgcgttccgg ctacgcggag attgccaccc cggtgcgcga gcggcgcgcc gaagccgccg
      301 gtctggaacg ccgcctctcg gacgtcgtca accaggcata cggcctcacg cccgaggaga
      361 tagacctgct ctggtcatcc gctccaccca ggatgccgaa gttcttgtag tggtagagta
      421 gcgtcatacg ggcatgaaga cgacggggta tttcgagaac gtgatcctcc aggtaaagag
      481 accgtacctc gaacgcgagt ggtgcgagcg ggtccgccga aaccccgagt caaccgccgt
      541 ggaagaggac accggccgta tccctcacta ggcctacgtc gaggaactcg acgcgctcgg
      601 gatgccaccg tacctgcgcg tcatcaccct tcaagatggc gagactatcc acaatgcgtt
      661 tccagacagg aacttcaccc gcgagcagag gagaaaaaac acgtgaagct caactattat
      721 cccgagacgg attcactgta cattcatctc tcggagaaac ccggcgcgga tgccgtggag
      781 atcgccgaac atgtagtcat agacgtggac gaaagaggca tcccggtcgg gatcgacata
      841 gacgcgaacg cgagcaagat cgtggacctc tcccggttgg atctcagcgg tatctctctg
      901 ggagatctgg cattcaccag cgaacctaaa gctcgggaag cggcaagcta ggggcagttt
      961 ccgggcaggc ggtgcgtagg gcgcagctca agctggcgca actagatcag gtcgggagcg
     1021 tggaagagtt gaggaagccg ccgggtaaca agctccacaa gctcggcggg gaccgggagg
     1081 gtcagtgggc catcaggatc aatgaccagt accggatctg cttccgcttc gacaacgaag
     1141 ctggagacgc ctacgacgtg gaggccacgg attaccacta gaggagaagc aatgggcgat
     1201 tggacggaag agatagagcg cgaggagcgg atacatcctc cggtgcatcc aggcgagatg
     1261 ctgcgcgagg agtggctggg gccgttgaat atgaacgcga accagctcgc gaaggctctc
     1321 ggggtagatc gccagaacat ttacgacatc gtgaacgaga agcgcggagt atctgcggag
     1381 atggccctga gactcgctcg ctggtcgggc atgcgccccg aattctggct cgggctacag
     1441 gagcattacg aattggagct cgccaggatg gaaagcggtg agaagataga gcgggaagtc
     1501 caaccgctga caaccgcgac agcggaacct caataaacca gcgcgaactc gtggctgcgc
     1561 gaagaggaag ctaagaaaat gaaagtctat tattcacccg agacagacag cctgaacatc
     1621 aaactccgaa ccggagaagc gggagtcgtc ggcgagatcg tgggcgaaga cctgaacgag
     1681 aacgtggtgc tccaccgcga cgacgccgga acgctctacg agatagaagt ggtcggaagc
     1741 gcgagcaagg tgttcgacct ctcccgcctg gaggtggaag gtctcccggt cggcgtctcc
     1801 cccgccatcc ggcaagagag caaagccttc tgagcgaaca gggtcgaacc cggatggaga
     1861 gcgccgcctc ccggctggaa tcattggagc gaaaagaggc cggacccctg tctctgggga
     1921 gtccggcctg agcgtttccg ttcgttgtca ggtgaaagct agttctgagc cgcggcgctc
     1981 tccgccacga tcccgctcgc cgtgaagctc tcggctccaa gcctcggagc ccgcgactgg
     2041 accactacct tgtcgccgcg aacgagatcc gtaagggtcg ccggggcgtc gtccttgacg
     2101 atcctggtgc tgtcgctcac cgagatctcg acctgctgtc ccacgtagga ctgggcgaac
     2161 ttgttggccc tctgcacgtc gaccgtcacc gagccgtcct gcacggaggc cacatcgccc
     2221 ttgaagacgt aggtgacgac cgggttgccc ttgtcggcgg gcttgccctt gccagcggga
     2281 cgccccttcg cctcggcgat cccggcgaac atcgcgacca tcagggctac taccagaacc
     2341 agagccatcg tcttcttcat cgttctctcc ttctgtgtga atatctgtta tcgcttgcag
     2401 gacagttatc ggatcacgcg gggtaggact ttagcgttct gagacgttct tcataagaat
     2461 ttccaaaact cttccgcgag cgcgcagcgc gctgcgagga ctcgtcttct ggcccggctc
     2521 cgagcccgtg cgtatactgg atagtggtgg cggacaatgg aggagtaagg ctgtgagcat
     2581 cgaggattac cggtcgttct accggcgttc gatagacgac ccggaggagt tctgggccga
     2641 gcaggcacgg atcatcgact ggcacaggga cttcgagagg gtgctggact tcagcaaccc
     2701 accgttcgcc cggtggttcg tcgggggcga gacgaacctg tgccacaacg cggtggaccg
     2761 gcacctggag gagcgcgggg atcagaacgc cctgatctac gtctccaccg agacggacga
     2821 gcgccgggag tacacctacc gggacctgca cgccgaggtc aacgccttcg ccgcgactct
     2881 aagggagctt ggcgtgggcc ggggggaccg ggtcatcatc tacatgccga tgatggcgga
     2941 ggcggtcttc gccatgctcg cctgcgtccg gctgggcgcc
//
LOCUS       DASSMB010000286         4577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1735216, whole genome shotgun sequence.
ACCESSION   DASSMB010000286 DASSMB010000000
VERSION     DASSMB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1735216"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VFJ72_16380"
     CDS             <1..895
                     /locus_tag="VFJ72_16380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET7481083.1"
                     /translation="ISLFITVGYLAWMAEELKLRAGWTALAMGMLLLSSPLLATLALG
                     QMYSVLALGLVVAWNADRRDRPMLSGVALGLVVALKPSLAPIVLWPLVRRRWRAFAAT
                     MAAGILGALVGAVVLGVDTTVQWFEILLRSPLSTYWDNASLPSSVARMFTENEFARPL
                     AVLPWLVPVAYIAGIGVIILTAVKVRHGSEAGLWALTAASLLASPIAWNNYLMLLAPG
                     ILLLMARGRVAVALLLLALQFIPPQWPLLWQGDDTALATLALTLYLYILAMHWAAFLT
                     FGAEREAEPEYGPDLATEKRG"
     gene            968..1864
                     /locus_tag="VFJ72_16385"
     CDS             968..1864
                     /locus_tag="VFJ72_16385"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET7481084.1"
                     /translation="MGSTISQRIVESLPFLDRVADRVQPKVQEAVAAGGTAVKNVLDG
                     TPMELPLHPVLTDVPAGSWTAAIVFDGLDVATGSRAMRNAADASLAMGIAGGLAAAVT
                     GFSDWRYLSGGSRRMGMAHGLLNVAGLALSTVSLILRLTGRRNAGRLAFLAGFSISGT
                     AMHLGGELSYNYGLRVNRAASQPTGPEGFTAVLPESELRGDELRAVSVEGAKVLISRS
                     QSGEICAMSAVCTHMGGPLEEGRREGDTVVCPWHGSRFELCTGKPLDGPAVFHQPGYE
                     VRVRDGEIEVRAGAENIQEKVR"
     gene            1870..2532
                     /locus_tag="VFJ72_16390"
     CDS             1870..2532
                     /locus_tag="VFJ72_16390"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET7481085.1"
                     /translation="MADDYVSPDWANAVLLTIDTQRDFTLRGAPAEIMGTEEIVPSMR
                     RLVGEFRLQNRPIIHVVRIYLPDGSNVDICRRGEIERGKRVVAPESDGAELVDELKPT
                     PETRLDAERLLSGELQRVAPAEWIMYKPRWGAFYQTPLEGHLQELGVDTVVVCGCNFP
                     NCPRATVYEASERDYRVALVADATSGLYERGAQELENIGVKLMDVEEVLKRLRVSALG
                     AP"
     gene            complement(2529..3143)
                     /locus_tag="VFJ72_16395"
     CDS             complement(2529..3143)
                     /locus_tag="VFJ72_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7481086.1"
                     /translation="MSLFYPTLGALVILAVFLFFIGRQNRDLLRVKAYREFLWRLIPA
                     LSTGVFIAGLPFVMNEATFDNYAPVLLVYLGGAAVLTAVMARAVAPEERRAGTAFRKG
                     DYEKAATLYEDLTERRPLPRYYSALSASLDATGDPHRALEAAEQAIKLDPKLGIAYYN
                     RASAFAALGEKTRARADLQRIFTVDSSRALRRAATEAMEILEKS"
     gene            complement(3140..3742)
                     /locus_tag="VFJ72_16400"
     CDS             complement(3140..3742)
                     /locus_tag="VFJ72_16400"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7481087.1"
                     /translation="MGAAAIPIVLVFLLVFFSWQTIYSANRDLVRAKAWGEMARRALP
                     FGALLLMAASLLIVRSFQESWVLLTWMVVSGVVIIGASRLAMPPTERKASKAFRSGDF
                     EKASDLYGELVEEQPLARNYAFLGAALGASERLDESVSSSTRAIEIDPQYGLAYYNRA
                     LVLQQQNKKSRAVKDLKKALEVDLPRRFRSAVRKRLEELS"
     gene            complement(3752..4345)
                     /locus_tag="VFJ72_16405"
     CDS             complement(3752..4345)
                     /locus_tag="VFJ72_16405"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HET7481088.1"
                     /translation="MDSSDSLHHALKEWAVAVRALEIGATALVVRKGGIREKAFAIED
                     SRFLLLPGYEHQRPELLKPEYRDLMDDLPKLSDEGPLRFSSFAEVAGAYEVSEAEELS
                     ALDEHHIWTPEYAESRFKWRPKKPLTVLLLRTYVLPETVELPYREEYGGCKSWIQLQD
                     SVSTEGARPALPDEEFERLTAPALAVLRRLEPAPVRS"
     gene            4387..>4577
                     /locus_tag="VFJ72_16410"
     CDS             4387..>4577
                     /locus_tag="VFJ72_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481089.1"
                     /translation="MTKTFELRREARMTQHETAHDTPHRKERDSMGEVEVPRDALFGA
                     QTRRALDNFPISDLRFPRR"
BASE COUNT          767 a         1422 c         1605 g          783 t
ORIGIN      
        1 gatctcactc ttcatcaccg tcggttacct ggcgtggatg gccgaggagt tgaagctgcg
       61 cgccggatgg acggcgctcg cgatggggat gctcctgctc tcttcgccgc tcttggcgac
      121 gctcgcgctc ggacagatgt attcggtgct ggcgctgggg ctggtcgtgg cctggaacgc
      181 cgaccgccgc gaccgcccca tgctctccgg cgtggctctc ggactcgtcg tcgcgctaaa
      241 accgtcgctc gccccgatag tcctctggcc gctcgtgcgg cggcgatggc gggccttcgc
      301 ggctacgatg gccgccggaa tcctgggggc gctcgtcggg gcggtcgtac tcggagtgga
      361 tacgacggtt caatggttcg agatactgct gcgctctccc ctcagcacct actgggacaa
      421 cgcttcgctc ccgtcctcgg tggcgcggat gttcaccgag aacgagttcg cccggccgct
      481 ggcggtgctg ccgtggctgg tgccggtggc ctacattgcg gggatcgggg tcataatcct
      541 gaccgccgta aaggtccgtc acggctccga ggccggactc tgggctctga cggccgcatc
      601 gctgctcgcc tcgcccatcg cgtggaacaa ttatcttatg ttgctcgcgc cgggaatact
      661 gctgctgatg gcgcgggggc gcgtggcggt ggccctgctg ctgctcgccc tgcagttcat
      721 cccgccgcag tggccgctac tgtggcaggg ggacgatacg gccctcgcta ccctggcgct
      781 aacgctctac ctgtatatcc tggcgatgca ctgggccgcg ttcctcacct tcggcgcgga
      841 acgggaggcg gagcctgagt acggaccaga cctcgctacc gaaaaacgcg gatagcgaac
      901 ggccccggca tactcgcgcg tggcataatc ttgccgtggg gttcactcgt gtagagaggt
      961 gaggggtatg ggatccacca taagccagcg catcgtcgag tcgctgccgt tcctggatag
     1021 ggtagcggac agggtccagc cgaaggtgca ggaggccgtc gcggcgggcg ggaccgcagt
     1081 caagaacgta ctcgacggca cgccgatgga gctgccgctt cacccggtgc tcacggacgt
     1141 accggctggc tcctggaccg cggccatcgt cttcgacggg ctggacgtgg cgacgggctc
     1201 cagggccatg cgcaacgcgg cggacgcctc gctggccatg gggatagccg gaggtctcgc
     1261 ggccgccgta acggggttct cggactggcg ctacctgagc ggcgggtcgc gccggatggg
     1321 catggcgcac ggcctgttga acgtcgccgg gctggccctc agcacagtct cactcatctt
     1381 gaggctgacg ggacgccgga acgccgggag gctggcattc ctggcggggt tctcgatcag
     1441 cggcacggcc atgcacctcg ggggagagct ctcctacaac tacggactgc gggtcaaccg
     1501 ggccgcctct cagcccaccg gccccgaggg attcacggcc gtcctgcccg agagcgagct
     1561 gcgcggcgat gagctgcgcg cggtttcggt ggagggcgcg aaagtcctga tcagccgctc
     1621 gcagagcggc gagatatgcg cgatgtccgc tgtatgcacc cacatgggcg ggccgctcga
     1681 agagggacgg cgcgagggcg atacagtcgt ctgtccctgg cacgggtcgc gcttcgagtt
     1741 gtgcaccggg aagccgctgg acggtccggc cgtcttccac cagccgggct acgaggtacg
     1801 ggtgagggac ggcgagatag aagtccgcgc cggggctgag aatatccagg agaaagtacg
     1861 gtaggacgct tggcggatga ctacgtgagc ccggactggg cgaacgcggt cctgctcacc
     1921 atagacacgc aacgggactt caccctccgg ggagcgccag cggagatcat gggtacagag
     1981 gagatcgtcc cgagtatgcg gcgtctggtc ggtgagttca ggctacagaa caggcccatc
     2041 atccacgtcg tgaggatcta cctgccggac ggctcgaacg tggacatctg ccgcagaggg
     2101 gagatagagc gcggcaagcg ggtagtggcg ccggagagcg acggggcaga gctggtggat
     2161 gagctgaagc caacaccgga gaccaggctc gacgcggagc gcctgctgtc cggggagcta
     2221 cagcgcgtgg cgcctgcgga gtggatcatg tacaagcccc gctggggcgc tttctaccag
     2281 actccgctgg aaggacattt gcaggagctt ggggttgata cggttgtggt gtgcgggtgc
     2341 aacttcccga actgtccgcg cgccacggtt tacgaggcga gcgagagaga ttaccgggtc
     2401 gcgctagtcg cggacgctac atccgggctc tacgagcggg gtgcgcagga actggagaac
     2461 atcggggtga agctgatgga tgttgaggag gtcctgaaga ggttacgagt atcggctctg
     2521 ggagcgccct agctcttctc caggatctcc atcgcttcgg tggcggcgcg gcgcagggcg
     2581 cggctggagt ctacggtgaa gatcctttgc aggtcggcgc gggcgcgggt cttctcgccg
     2641 agggccgcga aggcggaggc gcggttgtag taggcgatgc cgagcttcgg gtcgagcttg
     2701 atggcctgtt cggcggcttc gagggcgcgg tgggggtctc cggtggcgtc gaggctcgcg
     2761 gagagggcgg agtagtagcg agggagcggg cgccgctccg tgaggtcttc gtatagggtg
     2821 gcggcctttt cgtagtcgcc cttgcggaag gctgtcccgg cgcggcgctc ctcgggggcg
     2881 acggcgcggg ccatgacggc ggtcaggacg gcggccccgc cgaggtagac gaggaggacg
     2941 ggcgcgtagt tatcgaaggt cgcctcgttc atgacgaagg ggagtccggc tataaagacg
     3001 ccggttgaga gggccgggat caggcgccaa aggaattccc tgtaagcctt tacccgcagc
     3061 aggtcgcggt tctgtcgtcc gatgaagaac aagaagaccg ccaggataac gagcgcgccg
     3121 agtgtcgggt agaagaggct catgacagct cttcgagcct tttgcggacg gcgctcctga
     3181 agcggcgcgg caggtccacc tccagggctt tcttcaggtc ctttacggcc cggctcttct
     3241 tgttctgctg ctgcagcact agcgcccggt tgtagtaggc gagaccgtac tgcgggtcta
     3301 tctcgatggc tcgcgtcgag gaggagacgg actcgtcgag ccgctcggag gcgccgagcg
     3361 ccgccccgag gaaggcgtag ttgcgggcga gcggctgctc ctcgaccagc tcgccgtaca
     3421 gatccgaagc cttctcgaag tcgccggagc ggaacgcctt actggcctta cgctcggtgg
     3481 ggggcatcgc gagccggctg gcgcctatga tcacgacgcc cgagacgacc atccaggtca
     3541 aaagcaccca gctctcttgg aagctccgca cgataaggag gctcgcggcc atgaggagca
     3601 gcgcgccgaa cggcagcgcg cgccgcgcca tctctcccca cgccttcgcc cggaccagat
     3661 ccctgttcgc cgagtagatc gtctgccagc taaagaacac cagcaggaag accagcacta
     3721 ttggtatcgc cgccgcaccc aaggcgcgcc cctacgacct caccggcgct ggttcgagtc
     3781 tccgcaacac cgcgagcgcc ggagcggtca gccgctcgaa ctcctcgtcc gggagcgccg
     3841 ggcgcgcgcc ttcagtagag acggagtcct gaagctggat ccaggacttg catcccccgt
     3901 actcctcccg gtacggtaac tcgaccgtct ccggcagcac gtaggtcctc agcaggagca
     3961 ccgtcagagg cttcttgggg cgccacttga agcggctctc cgcgtactcc ggcgtccaga
     4021 tgtggtgctc gtcgagcgct gaaagctctt ccgcctccga gacctcgtag gcaccggcga
     4081 cctccgcgaa cgacgagaac cgcagcggac cttcatcgga cagcttcgga aggtcgtcca
     4141 tcagatcgcg gtactcgggt ttcaagagtt cggggcgctg gtgctcgtag ccgggtagca
     4201 ggaggaagcg cgagtcctcg atggcgaacg ccttctcccg gatgccgccc tttcggacga
     4261 cgagggctgt tgcgccgatc tccagcgccc ggacggcgac ggcccattcc ttcagggcgt
     4321 ggtgtagcga atcagaagaa tccaaaagat cacctctcgt ataatgctcc cgtaagtgaa
     4381 gattatatga ccaaaacgtt cgagcttcga cgggaggccc gcatgacgca gcacgagacc
     4441 gcgcacgaca ccccgcaccg caaggaacgc gactcgatgg gagaggtgga agtcccccgc
     4501 gacgccctct tcggcgccca gacccgtcgc gccctcgaca acttcccgat aagcgacctg
     4561 cgctttccca ggcgctt
//
LOCUS       DASSMB010000287         2860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_494131, whole genome shotgun sequence.
ACCESSION   DASSMB010000287 DASSMB010000000
VERSION     DASSMB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494131"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFJ72_16415"
     CDS             complement(<1..618)
                     /locus_tag="VFJ72_16415"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET7481090.1"
                     /translation="MNHLLAELGSSGVVLVSSYGYAGLAVLMLLGDMFMPVPTEIVLP
                     LVGFLALRGEFSFVAAAAVATAGSVAGALAVYALGHRLGEEPLRRFVGRFGRFVLVGE
                     PDLDRVLGYFERRGGMAVMVGRILPGTSSFVSVPAGLARMPLLRYVAYTTAGCAVWNV
                     AHIGLGWVLGTQWGLVREYAPVLQYAAMAAMVSGALWFVWRRKSLA"
     gene            complement(774..1691)
                     /locus_tag="VFJ72_16420"
     CDS             complement(774..1691)
                     /locus_tag="VFJ72_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7481091.1"
                     /translation="MDKSLWIADRVPGEVELAIWRSGDGPDGGQDPVICLHGITAQHR
                     AFTTAARLLEGERSLVGVDLRGRGDSDKPDSGYGLEAHASDVVRVLDHLGLDRADIAG
                     HSMGGFVGLQTALTYPERVRSLVLLDGGWPRIEVSPDDMTEEQKEEAAAVQEGLARAF
                     RRLDMVFETPDAYLDFWFPGQDLTMGDLPPDLADYYTYDLGRVEGGYQPKCSSAAAKE
                     DSTWLSERAPTAEQMKGVSCPVALVRPTAGFFPDSDPLIPEETRAVMAETLGLRSEVL
                     LQGANHYTMMWEPFAERWAELLSSDSWFR"
     gene            1763..2764
                     /locus_tag="VFJ72_16425"
     CDS             1763..2764
                     /locus_tag="VFJ72_16425"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7481092.1"
                     /translation="MSYYENVLDAVGNTPLIRMSRIERDEGIEATLLAKVEYLNPGGS
                     VKDRPAIRMLEVAEEKGLLKPGGTVVEPTSGNTGAGLALAAAVKGYKCICVMPEKASK
                     EKQDFLKAMGAEVVVTPSLPPDDPESYYSVAERLANEIPGGFKPGQYENPANPLAHYE
                     TTGPELWKQTAGRITHFVAGMGTCGTITGTGRYLKEVNPEVQVVGADPEGSIFSDPDN
                     IHKYAVEGVGEDFYPGNYDGGVVDEVIQVDDRESFLMTRRLMAEEGLFVGGSCGMAAV
                     AAVRAAKGLPQSAVVVVLLPDTGRNYVSKVFDDEWVLANSTATKEDLEKPYRIFGEG"
BASE COUNT          536 a          949 c          966 g          409 t
ORIGIN      
        1 cgccagactc ttccggcgcc acacgaacca gagcgcgccc gagaccatgg ccgccatcgc
       61 cgcgtactgg agtaccgggg cgtactccct gacgagcccc cactgggtcc ccaggaccca
      121 tccgagccct atgtgcgcca cgttccagac ggcgcagccg gccgtggtat aggcgacgta
      181 tctgagaagc ggcatgcgcg cgagcccggc gggcacggag acgaagctgc tcgtgcccgg
      241 caggatcctg ccgaccatca cggccatccc gccgcgccgc tcgaagtacc cgagcacccg
      301 gtccaggtcg ggctcgccca ccagaacgaa gcgcccgaac cgcccgacaa agcgccggag
      361 cggctcctca ccgagccggt gtcccagagc gtacacggcc agcgccccgg ccacggaccc
      421 cgccgtggcg acagccgccg ccgcgacgaa cgagaactcc ccccggaggg ccaggaaacc
      481 cacgagcggc agcacgatct ccgtggggac tggcatgaac atgtccccca ggagcatcag
      541 caccgccaga cccgcgtacc cgtaggagct tacgaggacg acgccggacg aaccaagttc
      601 ggcgagcagg tggttcatca ggttctctcc gtacctctct attagcgagc gttcaagaag
      661 tgtacggagc cgggagcgcg agagatcaca ccggtcgggg tcagccggac gggctacgaa
      721 acgcgccgtc gtatttacgg cgtccgattc gccgctgtgt tgtctccagc ctactaccgg
      781 aaccagctat cggagctcag aagctcagcc caccgctccg cgaagggctc ccacatcatc
      841 gtgtaatggt tcgctccttg gagcaggact tccgagcgga ggcccagggt ctcagccatt
      901 accgctcggg tctcttcggg gatgagggga tcgctgtcgg ggaagaagcc cgccgtcggc
      961 cggacgaggg cgacagggca ggagacgccc ttcatctgct cggcggtcgg ggcgcgctcg
     1021 gagagccagg tagagtcctc tttggcggcg gcggaggagc atttgggttg atagccgccc
     1081 tccaccctac cgaggtcgta ggtgtagtag tcggcgaggt ccggcggcag gtcgcccatc
     1141 gtgaggtcct gtccggggaa ccagaagtcc aggtaagcgt ccggggtctc gaagaccatg
     1201 tcgaggcggc ggaaggcgcg ggccagcccc tcctgcacgg cggcggcctc ctccttctgc
     1261 tcctcggtca tgtcgtcggg ggagacttcg atccgcggcc agcctccatc gagcaggacg
     1321 agggatcgga cgcgttcggg gtaggttagc gcggtctgga ggccgacgaa gccgcccatc
     1381 gagtgcccgg ctatgtccgc gcggtctagt ccgaggtgat ccaggacccg taccacgtcg
     1441 gatgcgtggg cttcgagacc gtagccggag tcgggcttgt ccgagtcgcc ccggccccgc
     1501 aggtctacgc ccaccagaga tctttcccct tcgaggaggc gggcggcggt ggtgaaggcg
     1561 cggtgctggg cggtgatgcc gtgcaggcag atgacggggt cctgaccgcc gtccgggccg
     1621 tcgccggagc gccagatcgc cagctccacc tcgccgggta cgcggtcggc gatccacaag
     1681 ctcttatcca tgaccgcagt atacgcgaga gcgggttcgg ggctgcggcg ggtggtacat
     1741 taccagcatg aataactccg ggatgagcta ttacgagaac gtgctggacg ctgtggggaa
     1801 cacgcccctg atccgtatga gccgcatcga acgcgacgag ggcatcgagg cgacgctgct
     1861 ggcgaaggtc gagtacctga accccggcgg ctccgtgaag gaccggcccg ccatcaggat
     1921 gctggaggtc gccgaggaaa aaggtctcct gaaacccggc ggcacggtcg tcgagccgac
     1981 gagcggcaac accggcgcgg ggctcgcgct cgcggcggcg gtcaagggct acaagtgcat
     2041 ctgcgtgatg ccggagaagg ccagcaagga gaagcaagac ttcctgaagg ctatgggcgc
     2101 ggaggtggtc gtaaccccga gcctgccgcc cgacgacccg gagagctact actccgtcgc
     2161 cgagcggctc gccaacgaga tccccggcgg cttcaagccc ggacagtacg agaacccggc
     2221 caaccccctc gcccactacg agacaacggg accagaactc tggaaacaga ccgccggccg
     2281 catcacccac ttcgtcgccg gcatgggcac ctgcggcaca atcaccggca ccggacgata
     2341 cctgaaagag gtcaatcccg aagtgcaggt cgtcggcgcg gacccggagg gctcaatctt
     2401 ctccgacccg gataacatcc acaagtacgc cgtcgagggc gtgggagagg acttctatcc
     2461 ggggaactac gacggcggcg tggtggacga ggtcatccag gtggacgacc gcgagtcgtt
     2521 cctgatgacc cgccgcctga tggcggaaga agggctcttc gtcggaggct cgtgcgggat
     2581 ggccgccgtc gccgcggtgc gggcggcgaa ggggctgccg cagagcgccg tggtcgtcgt
     2641 gctcttgccc gacaccggcc gcaactacgt ctccaaggtc ttcgacgacg agtgggtgct
     2701 cgccaactcg accgcgacaa aggaagacct cgaaaaaccg taccggatct tcggggaggg
     2761 atgatatgaa gttctccacg cgagccatcc acgtcgggca agaggcagac ccggcgacag
     2821 gcgccacgat agtcccgatc taccagacct ccacctacac
//
LOCUS       DASSMB010000288         3416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1506991, whole genome shotgun sequence.
ACCESSION   DASSMB010000288 DASSMB010000000
VERSION     DASSMB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1506991"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VFJ72_16430"
     CDS             <1..549
                     /locus_tag="VFJ72_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481093.1"
                     /translation="EIYALQVHFNPLFLVYVALLGGSLYALIGGLATTDLAGIKARFT
                     GRTPVKTASIFLAAVAVLFYFAWLSEVVPALVAGEVPQSVTQNGTPTNAVHVLDMAWI
                     LPAAILTAIWLWRRRAIAYAIAGALLTFFSLLALAIMAMMISMSLFGQPVALGMAAVF
                     GVVSATGLGTLIWYMRAFRDRM"
     gene            complement(684..1427)
                     /locus_tag="VFJ72_16435"
     CDS             complement(684..1427)
                     /locus_tag="VFJ72_16435"
                     /inference="COORDINATES: protein motif:HMM:NF021998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HET7481094.1"
                     /translation="MIKQEANTATSSQHSETSRGHYVLELCGILAFTALAILIGVEAY
                     EGMANFGYPWLLPILAIIAYLAADLLSGIVHFLADNFGSYDTPVIGPGFIDAFREHHV
                     DPKGIVGHDFVDTNGNNSLVAVPFMLLIWLAVPVETALWGYLLGAFSLFLCLATFLTN
                     QFHKWAHMEAPPAPIAWLQRRGFILSAEHHDIHHASPYDTYYCITAGFWNPLLDRLRF
                     FERTERLIRRVVPGTDPLLRAERDGSLND"
     gene            complement(1766..2716)
                     /locus_tag="VFJ72_16440"
     CDS             complement(1766..2716)
                     /locus_tag="VFJ72_16440"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7481095.1"
                     /translation="MKIGYFLSSEEFGPHQQIEQARMADEAGFEGLWISDHYHPWIDA
                     QGQSPFVWSVIGALSQVTKLPVTTAVTCPTVRIHPAILAQAAATSAVMHEGRFRFGIG
                     SGEALNEHILGDAWPSTDTRLEMMEEAVEVIRLLWEGGTQSHEGKYYLVDNARIYTLP
                     DEPPPILISGFGPKAIELAGRIGDGYVNVAPDRDMLDLFRSSGGGDKPAHGAFKVCYG
                     DDEAECVRTAHRLWPNEALPGELAQVLPTPAHYEQATQLVTEDMISESVPCGPDPEPH
                     RALIRQFADAGYDEVYVQQIGPNQKQFFDLYEKEILPEFR"
     gene            complement(2816..>3416)
                     /locus_tag="VFJ72_16445"
     CDS             complement(2816..>3416)
                     /locus_tag="VFJ72_16445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7481096.1"
                     /translation="SVRPLGEALAAEGFTVAGPRLKGHGTSMEDHARSTARDWISSIE
                     EDLAWLEERTNSVFFAGLSMGGMFSLYFGGMRPDLIRGIIPINACVFLNNPDLARIVF
                     DPKAPPSVPGVGSDIKAPGVVLHARSMPRQARSRHREALRGERLAQRPDALRGPREPV
                     RDEHSDLVPLEAKRFRALHDLLLHSRTSHHSGSQFPEVL"
BASE COUNT          601 a         1030 c         1149 g          636 t
ORIGIN      
        1 gaaatctatg ctctccaggt ccacttcaac ccgttgttcc tcgtctacgt cgcgctgctc
       61 ggaggttctc tgtacgccct gatcggaggt ctcgccacta ccgatctagc agggatcaag
      121 gcgcgcttca ccggaagaac gcccgtgaag accgcgagca tcttcctggc tgcagtggct
      181 gttctgttct acttcgcgtg gttgagcgag gtcgtccccg ccctggtcgc gggagaggtg
      241 ccgcagagcg tcacgcagaa cgggaccccg accaacgccg tccacgtgct ggacatggcg
      301 tggatactcc ccgcggcaat cctgacggcc atctggctgt ggcgcaggcg agcgatagct
      361 tacgcgatag cgggcgccct gctcaccttc ttctcgctgc tcgcgctggc aatcatggcg
      421 atgatgatat ccatgagcct attcggccaa cccgtcgcac tcgggatggc ggcggtcttc
      481 ggcgtcgtct cagcgaccgg tctggggacg ctcatctggt acatgagagc attcagagac
      541 cgaatgtagc ctggagggag cgcagaatct ccgtgcgctt cactgcgact tccggcacgt
      601 cccatggccc agagtaggcg tgcggtaagt ggcgcataca gggctgattc ctgctcccat
      661 tcgccgcgcc tcaccgtatg aggtcagtcg ttaagactac cgtcgcgctc ggctcgcagg
      721 agcgggtccg taccggggac tacgcgccgg atcaggcgct ccgtccgctc gaagaagcgg
      781 agcctatcca gcagcgggtt ccagaaaccg gcggtgatgc agtagtaggt gtcgtagggg
      841 ctagcgtggt ggatgtcgtg gtgctcggcg gagaggatga agccacgcct ctggagccat
      901 gcgatagggg ccggaggagc ttccatgtgc gcccacttgt ggaactggtt ggtcaggaac
      961 gtcgcgaggc ataggaagag ggagaacgcc ccgaggagat aaccccagag cgccgtctcg
     1021 acgggtacgg cgagccagat gagcagcatg aacggcacgg cgaccaggct attgttgccg
     1081 ttggtgtcca cgaagtcgtg accgacgatc cccttggggt cgacgtggtg ctcgcggaag
     1141 gcgtctatga agccggggcc gatcaccggg gtgtcgtagg agccgaagtt gtcggccagg
     1201 aagtgaacga tgcccgagag caggtccgcc gccaggtacg cgatgatggc gaggatcggc
     1261 agcagccacg ggtagccgaa gttcgccatc ccttcatacg cctccacccc gatcaaaatg
     1321 gcgagcgcgg tgaaggccag aatgccgcag agctcaagga cgtagtgccc tctgctagtc
     1381 tcggaatgtt gactgctcgt cgctgtattc gcttcctgtt tgatcacaaa agcctccttc
     1441 atggcaaggt ttcagtcttt ggcgctatcg ggacaacggg gacaacgttt tggcctgatg
     1501 cggactccga aaatcggatc tttggtagct atacggatca ggcagacgtg ccgatcacag
     1561 atggagcaga agcgccatga cgaaagcggg cgctggttct tccaccggca ccgattccct
     1621 gtttttcgcc cttactgttc gggtggccgg ccgctggagc cagcctcttc tgcgccagga
     1681 cttgtcgtga agaacaatgc gatcttgcat ggccggcgca tccgaattct tctatgcgct
     1741 ccgggaagga ggttatgttc tcacgctagc ggaactccgg cagtatctcc ttctcgtaaa
     1801 ggtcgaagaa ctgcttctgg ttcgggccga tctgctgcac atagacttcg tcatagccgg
     1861 cgtcagcgaa ctggcggatc aacgcgcggt gcggctccgg gtccgggccg caaggtacgg
     1921 actccgagat catgtcctcc gtgacgagct gcgtggcctg ctcgtagtgg gcgggggtcg
     1981 gcaggacctg ggccagctct ccgggcagcg cctcgttcgg ccagagccgg tgcgcggtcc
     2041 tgacgcactc ggcctcgtcg tcgccgtaac agaccttgaa ggcgccgtgg gcgggcttgt
     2101 ctccgccgcc ggaggagcgg aagaggtcga gcatgtccct gtcgggggcc acgttgacgt
     2161 agccgtcgcc tatcctgccg gcaagctcga tggcctttgg gccgaagccg gagatgagga
     2221 tcggcggcgg ctcgtcaggg agcgtgtaga tcctcgcgtt gtccaccagg tagtatttgc
     2281 cctcgtgact ctgcgtgccg ccctcccaca acaggcggat tacctcgacg gcctcctcca
     2341 tcatctccag gcgcgtatcg gtcgagggcc aggcgtcgcc gaggatgtgc tcgttcaggg
     2401 cctcgccaga gccgatgccg aagcggaaac gtccctcgtg catcacggcg gaggtcgcgg
     2461 cggcctgggc caggatcgcc gggtggattc ggacggtcgg gcaggtcacg gcggtggtta
     2521 cgggaagctt cgtcacctgg gagagcgcgc cgatgacgct ccacacgaac gggctctggc
     2581 cctgggcgtc gatccaggga tggtagtggt ccgagatcca caacccctcg aatcccgcct
     2641 catcggccat cctggcctgc tctatctgct ggtgtggccc gaactcctcg ctggacagaa
     2701 agtacccgat cttcacgcaa ccctcctggc gtgccacggt catggacaga gcggatacta
     2761 cccccggctc ccaagaaggt aaccgttgct cgaaagcgac gggagattcc ggtagctaca
     2821 atacctccgg gaattgtgaa ccagagtgat gggaggtcct ggaatggagc agcaagtcat
     2881 ggagggcgcg gaaccgtttc gcttcgaggg ggacgagatc ggagtgctcg tctcgcacgg
     2941 gttcacgggg accacgcaga gcgtccggcc gctgggcgag gcgctcgccg cggagggctt
     3001 cacggtggcg ggaccgcgcc tggcggggca tggaacgagc atggaggacc acgcccggag
     3061 cctttatgtc cgagccgacg cccggcacgc tcggcggggc cttcgggtcg aatacgatgc
     3121 gggccaggtc cgggttgttc aggaagacgc aggcgttgat ggggatgatg ccccggatca
     3181 ggtccggccg catgccgccg aagtagagtg agaacatgcc tcccatggag aggccggcga
     3241 agaacacgga gttggtgcgc tcttcgagcc aggcaaggtc ttcttcgatg gaggagatcc
     3301 aatctcgggc cgtgctgcgg gcgtgatctt ccatgctcgt gccgtgcccc ttgaggcgcg
     3361 gtcccgccac cgtgaagccc tccgcggcga gcgcctcgcc cagcggccgg acgctc
//
LOCUS       DASSMB010000289         6940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1737261, whole genome shotgun sequence.
ACCESSION   DASSMB010000289 DASSMB010000000
VERSION     DASSMB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1737261"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFJ72_16450"
     CDS             <1..344
                     /locus_tag="VFJ72_16450"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7481097.1"
                     /translation="ALAEGLAYSAAVAIDNARHHVSEAELARELVQRARQDQRTAAPL
                     GRPDAPELTARQLEVLRLLSDGRSAKEIGADLYLSQATVRNHIRSLLQALGAHSQLEA
                     LARARKAGLIP"
     gene            complement(373..753)
                     /locus_tag="VFJ72_16455"
     CDS             complement(373..753)
                     /locus_tag="VFJ72_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481098.1"
                     /translation="MGGQRTSMIERLVHRIEEHIEEWRKEEAARHRAAEENRDRLWAA
                     AAERERLLVETINEEESRRDSLEEITKQHRLVFVAHSEEVAETLEEFASEGDRLVKVI
                     PGRQGSYAKEVGVKGSWLVFEAPE"
     gene            complement(793..2058)
                     /locus_tag="VFJ72_16460"
     CDS             complement(793..2058)
                     /locus_tag="VFJ72_16460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7481099.1"
                     /translation="MINGSKPNIAVLGGGFGGLEAAFYMRMKLQDRANITLVSDKDYF
                     LFKPNTIYIPFGLDPDKLKIDLKRPTRRKDITFIKDRVREVDPDSKMVATEGRKISYD
                     YLVVATGSDMRPEEVPGLRQHAETIWTPQEMLKLRLAYYELLEEAREGRNKRVLFLVA
                     PNNKCAGPLYEMVMMLDTWLRRKKVRANVDITWSTYEQSYIQAFGPRLHEVVTKEFER
                     RVIAGHTKYVAEKVEDGEVHYANGEVLPYDLLITFSPYIAEMTYPTLPGDDRGFITTD
                     LKTRQVVGHPDIYAVGDAGDFPVKQAFLAFMQGDAAAEHLSARVLGTEPEISFQPTSM
                     CVMEEFDRATFAQVPLRLTGVPEKPIDVDPDAMDMYKVGTSVPWRLGKKMIGLYLPMR
                     FGWGNPFHAGLPWKGMEVGLKAMSGVLAK"
     gene            complement(2119..2724)
                     /locus_tag="VFJ72_16465"
     CDS             complement(2119..2724)
                     /locus_tag="VFJ72_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7481100.1"
                     /translation="MSAQAINSRESQIPEPNVSKVLFADTRLAPVWLIVRVYLGYLWL
                     TAGWEKLTDPAGVWIGSKAGAAVAGFAQGAIQQTTGEHPQVTGWYAGFLKDIVVPNAA
                     LFSYLVTFGEVLVGLGLIFGLFTGIAAFFGGTMNASYIFAGVAGANPLMFILATWIVL
                     AWRVAGYWGLDRWVLPRVGVPGSPGTLFRGRHREDTGTAHA"
     gene            complement(2787..3041)
                     /locus_tag="VFJ72_16470"
     CDS             complement(2787..3041)
                     /locus_tag="VFJ72_16470"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET7481101.1"
                     /translation="MAFARFMSEPTGRILRVVAGLALIVLGFLIGGTAGVLIVIVGLV
                     PVAAGAFNYCLIGPLIGGYFDGRKNLEGRPPRTHRGAHQH"
     gene            3416..4477
                     /locus_tag="VFJ72_16475"
     CDS             3416..4477
                     /locus_tag="VFJ72_16475"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7481102.1"
                     /translation="MEAFREEALGVLEHVFGDRLKFGPGREADLASGGSLAQVSPVNV
                     GEVETLARLAGAHGFPVTVAGAGLSGEVGAEEGSVVVRFDLMRSTRLPEGEELWAEAE
                     PGALWLTLDNELRLRGKGLAVYPTSAPRATVGGWLATGGLGVGSFEYGWLHDNVLSAS
                     VVLPGGERREVPGEELRSAVGEERTGIVVSARLRARRADADVPFAATFEDPEGLIGAL
                     DAIVGEGLPFWHLAFLSPGMTRARGLGEDYMIFGAYPEPRAARIEAGARSIFDSHNGR
                     TLPAAETHRAWGERFFPVAPSHPTPEASRELIAAPDLARTIRDERLRAGEVAAQGTVV
                     SSGDALLLSFDTQRGGLAR"
     gene            complement(4530..5675)
                     /locus_tag="VFJ72_16480"
     CDS             complement(4530..5675)
                     /locus_tag="VFJ72_16480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7481103.1"
                     /translation="MRVERVFSVEDVDETAWRALEPPDFPFFDLEFLRALERSGSIGG
                     KSGWSPVYLVCEDEGRVLGALCLYIKTDSYGEYIFDWEWARAYGEHGLPYYPKLVSAV
                     PFTPATGPKLLVRSDASEAERAAVVGALMDSAGRLGDECRVSSSHALFLPERELEEFA
                     RRGYAVRHSMQFHWRNRGYGAFSDYLDALDGKKRRQVARERRQLGEEGLEIERLNGDA
                     LPSEYGALMYRYYLRTLDGKWGVPYLNKAFFDEVFRTMKDRILFVLARDGETGRPVAG
                     ALNFYKGRTLYGRYWGASQARRNLHFELCYYQEIEFAIERGLELFEAGAQGEHKLARG
                     FLPTITYSAHDIRHPAFRHAIAQYLDEERELLSGVMAEYALHDPYRC"
     gene            complement(5888..6262)
                     /locus_tag="VFJ72_16485"
     CDS             complement(5888..6262)
                     /locus_tag="VFJ72_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7481104.1"
                     /translation="MIGSDEIYSERFCPRYQRAVEIVGRRWSGAILRAMLAGASRFGE
                     IQETIPDLSNRMLSERLREFEAEGIVERVVVPERPVRVEYRLTEKGRALNEVVEALSR
                     WSQDWVELEGAGEDSGRPAAAS"
     gene            6360..6770
                     /locus_tag="VFJ72_16490"
     CDS             6360..6770
                     /locus_tag="VFJ72_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET7481105.1"
                     /translation="MLDVGLLVLRVVLGVIFAAHGAQKLFGSFGGPGLKGTAGFHEQL
                     GIKPPYLMAILAGLAEFAGGILVAAGLLTPLAAVALIVTMAVAVFAVHLKNGFFAMNG
                     GYEFNLALAGMALTLLLTGAGAISLDAALGIVLW"
BASE COUNT         1153 a         2290 c         2210 g         1287 t
ORIGIN      
        1 tggcgctcgc cgaggggttg gcctacagcg cggccgtcgc catagacaac gcccgccacc
       61 acgtctccga agcggagcta gcgcgggaac tggtccagcg cgcgaggcag gatcagcgga
      121 cggcggctcc cctgggccga ccggacgctc ccgaactgac cgcccgccaa ctcgaggtcc
      181 tgcgtctgct ctccgatggg agaagcgcga aggagatcgg ggcggatctc tacctatcgc
      241 aggctaccgt gcggaaccac atccgctcgc tcctgcaagc tctaggggcg cactcccagc
      301 tagaggccct ggcgcgagcc cgcaaggccg gtttgatccc gtagcgtcgg tctggagcgc
      361 ctttacttcc ctttattctg gggcctcgaa gacgagccag gagcccttca cgcccacttc
      421 cttcgcgtag cttccctggc ggccggggat cacctttacc agccggtctc cctcgctggc
      481 gaactcctcc agcgtctccg ctacctcctc cgagtgcgcc acgaacacca gccggtgctg
      541 cttggtgatc tcttcgagcg agtcgcgcct cgactcctct tcgtttatgg tctcgaccag
      601 caacctctcc cgctcggcgg cggcggccca cagcctgtcc ctgttctcct ccgctgccct
      661 gtgacgagcg gcctcctcct tgcgccactc ctcgatgtgc tcctcgatcc tatgcacgag
      721 cctctcgatc atggacgtgc gttgccctcc catctcactc ctcctttgtc tgatcgtttt
      781 catcataatc cctcacttgg ctagcacacc actcatcgcc ttgaggccca cctccattcc
      841 cttccacggg agacccgcgt ggaacgggtt gccccacccg aagcgcattg gcaggtagag
      901 cccgatcatc ttcttcccca gccgccacgg gaccgaggtg ccgaccttgt acatgtccat
      961 cgcgtccggg tccacatcta tcggcttctc cggcacgccc gtcagtcgca gcgggacctg
     1021 ggcgaaggtc gcccggtcga actcctccat gacgcacatg ctggtgggct ggaaggagat
     1081 ctccggctcg gtgcccagca cgcgggccga gaggtgctcg gcggcggcgt ctccctgcat
     1141 gaaggccagg aaggcctgct tgaccgggaa gtcacccgcg tccccgacgg cgtagatgtc
     1201 ggggtgtccc accacctggc gggtcttgag gtccgtggtg atgaagcccc ggtcgtctcc
     1261 cggcagcgtg ggataggtca tctcggcgat gtagggagag aaggttatga gcaggtcgta
     1321 gggcaggact tcgccgttcg cgtagtggac ttcgccgtcc tccaccttct cggcgacgta
     1381 tttcgtgtgt ccggctatca cccggcgctc gaactctttc gtgacgactt cgtgcagacg
     1441 gggaccgaag gcctggatgt agctctgctc gtaagtggac caggttatgt ccacgttggc
     1501 gcggaccttc ttacggcgca gccaggtatc gagcatcatg accatctcgt agagaggtcc
     1561 ggcgcacttg ttgttcgggg cgacgaggaa gagcacccgc ttgttacgac cttcgcgggc
     1621 ctcttcgagc agctcgtagt aggccagcct gagcttgagc atctcctggg gcgtccagat
     1681 ggtctcggcg tgctgcctga gtccgggcac ctcctctggg cgcatgtcgg agccggtagc
     1741 cacgacgaga tagtcgtaag agatcttccg gccttccgtc gcgaccatct tcgagtccgg
     1801 gtccacctcg cgcacccggt ccttgataaa cgtgatgtcc ttgcggcggg tgggacgctt
     1861 caggtcgatc tttagtttat cgggatcgag cccgaagggg atgtagatcg tgttcggttt
     1921 gaataggaaa tagtctttgt ccgagacgag cgttatgttc gcccggtctt gcagcttcat
     1981 tcgcatatag aaggccgcct ctagccctcc gaaccctccg ccgagtaccg ctatgtttgg
     2041 tttgcttccg tttatcattg ccgatgctcc tttcggagcg gggtgactct ccgtggagag
     2101 ccaccccgtc gtgagttttc aggcgtgagc tgtgcctgtg tcttctctgt gccgtccgcg
     2161 gaagagtgtt ccgggggaac cggggacgcc gacccggggc agcacccagc ggtcgaggcc
     2221 ccagtacccg gcgacacgcc aggcgagcac gatccaggtc gccaggatga acatcagcgg
     2281 gttggctccc gcgaccccgg cgaatatgta gctggcgttc atcgtgccgc cgaagaaggc
     2341 cgcgatgccg gtgaacaggc caaatatcaa accgagcccg accaggacct cgccgaaggt
     2401 aaccaggtag gagaacaacg cggcgttcgg aactacgatg tccttcagga agcccgcgta
     2461 ccatccggta acctggggat gctcgccggt ggtctgctgg atcgcgccct gggcgaagcc
     2521 cgcgacggcg gctcccgcct tgctcccgat ccagacgccc gcggggtctg ttagcttctc
     2581 ccagccggct gttagccaca ggtagcccag gtacacccgg acgatcaacc ataccggggc
     2641 caggcgagta tcggcgaaca ggactttgct cacgtttggc tcgggtatct gggactctct
     2701 ggaattaatt gcttgcgcgc tcattgattc tcctctctac agtgtgggcc gggctcgggg
     2761 cgagcccggc ttgcgggacc ggcctctcag tgctggtgcg ctccccgatg ggtccggggc
     2821 ggacggcctt ccaggttctt gcgtccgtcg aagtagcctc cgatcagcgg accgatcaga
     2881 cagtagttga aggcccccgc cgcgaccgga acgagaccga cgatcacgat caggacgcct
     2941 gcggtcccac cgatcaggaa ccccagcacg atcagcgcaa gccccgctac tacccggagg
     3001 atccggccgg taggctcgga catgaatctc gcgaatgcca tgacttctca cctccttctt
     3061 tgctccggtt tctctactcg aaatctaccc ctagagcttg tgcctgcctt gatcagcctg
     3121 gctcatttgc ctggttagtt ttgaccaagt ctcgccctat accgccgctg gcacggaatc
     3181 cgaggggcgc gtggtgatcg agaactcgac cgagcggcgg ctccgctctc cgccaggctc
     3241 tgtggagaac gggtagatcc cctcaccggc aacgcaagag cgcgcgagag gacgcaagag
     3301 gagcgggaga gaaatagtca atctggatgg tagggcggtc aagatagatc atgcgtcgcc
     3361 gcttaccggg tagtatgctg ccgaactacg gctgatcgga aggaaggtgt gccggatgga
     3421 agcttttcgg gaggaggcgc tgggtgttct ggagcacgtc ttcggggacc gcctcaaatt
     3481 cggaccgggc cgggaagcgg acctcgcctc cggcggttcg ctcgcccagg tatcgccggt
     3541 caatgtcggg gaggtggaga cgcttgcgcg gctagccggg gcgcacggtt tcccggtgac
     3601 cgtcgccggc gccgggctgt cgggagaggt cggcgcggag gaggggagcg tcgtggtccg
     3661 gttcgacctg atgcgctcga cccggctccc ggagggggag gagctctggg ccgaggccga
     3721 gcccggcgcg ctgtggctca cgctggacaa cgagctgcgg ctgaggggca aagggctggc
     3781 cgtatatccc acgagcgcgc cgcgggctac cgtgggaggc tggctcgcca cgggcggtct
     3841 cggcgtgggc tccttcgagt acggctggct ccacgataac gtgctctccg cgagcgtcgt
     3901 gctgccggga ggcgagcgcc gcgaggtgcc cggtgaagag ctgcgttccg ccgtcgggga
     3961 agaacggacc gggatcgtcg tgagcgcccg ccttcgcgcc cgccgcgccg acgccgacgt
     4021 gccgttcgcc gctaccttcg aagacccgga gggtctcatc ggcgccctgg acgccatcgt
     4081 cggagagggc ctgccgttct ggcatctggc cttcctgagc cccggcatga cccgtgcccg
     4141 cggtctcggg gaagactaca tgatattcgg cgcctatccg gagccgcgcg cggcaaggat
     4201 cgaagccggc gcgcgttcca tcttcgactc acataacggg cgcacactgc ccgccgccga
     4261 gacacaccgc gcgtggggcg agaggttctt cccggtcgcg ccttcccatc cgaccccgga
     4321 agcgagccgc gagttgatcg ccgcccccga cctcgcccgg acgatcaggg acgagcgtct
     4381 gcgcgccggg gaggtcgccg ctcagggaac cgtcgtaagc tcgggcgacg cccttctcct
     4441 ctcgttcgac acgcagcggg gaggcctcgc gcggtagcgc cacgcgcaag cctccggtga
     4501 tgccgcccgg ccaaacggat atccgttgtt cagcatctat acggatcgtg gagcgcgtac
     4561 tccgccatga ccccggagag cagctctctc tcctcgtcga ggtactgcgc gatggcgtgt
     4621 ctgaatgccg ggtggcggat gtcgtgcgcg ctgtaggtga tggtcgggag gaagccgcgc
     4681 gctagcttgt gctccccctg cgcgccggcc tcgaagagct ccaacccacg ttcgatggcg
     4741 aactcgatct cctggtagta gcagagctcg aagtgcaggt tgcgccgcgc ctgcgaggcg
     4801 ccccagtagc gcccatagag cgtccggcct ttgtagaagt tgagcgcgcc cgcgaccgga
     4861 cgccccgtct caccgtcacg cgctagcacg aacaggatac ggtccttcat ggttcggaag
     4921 acttcatcga agaaagcctt attcaggtac gggacgcccc actttccatc gagcgtcctg
     4981 aggtaatagc ggtacatcag agcgccgtac tccgacggga gagcgtctcc gttaaggcgt
     5041 tctatctcca gcccttcctc gccgagttgg cggcgctcgc gcgctacctg gcggcgcttc
     5101 ttgccgtcga gcgcgtccag gtaatcggag aacgcgccgt atccccggtt gcgccagtgg
     5161 aactgcatcg agtgccgcac cgcatatccg cgccgcgcga actcttccag ctcccgctcg
     5221 ggcaggaaga gcgcgtgcga tgagctgacg cggcactcgt cgccgagcct cccggccgag
     5281 tccatcaggg ctccgaccac cgccgcgcgt tccgcctcac tggcatcgga ccgcacgagc
     5341 agcttgggtc cggtggcggg tgtgaacggg acggccgaga cgagcttggg gtagtacggc
     5401 agcccgtgct ccccatacgc ccgcgcccac tcccagtcga agatgtattc gccgtaggag
     5461 tcggtcttga tatagagaca tagcgcgccg agcacgcgcc cctcatcctc gcagacgagg
     5521 tagaccggcg accacccgct cttgcccccg atgctgccgg agcgttcgag cgcccgcagg
     5581 aactccaggt cgaagaacgg gaagtccggc ggctccagcg cgcgccacgc cgtctcgtcc
     5641 acgtcctcga cgctgaagac ccgctcgacc cgcatcacgc taccggagag gagacgggga
     5701 acggctcatt ccgcgcgcct cgcggaatgc ttcgcggaga agcggtcgtc ttgacgctcg
     5761 tgggcaactc ctctcgaaag ccggacacga caacgggtat tttactcgcc cccggaccac
     5821 tgaaaagcac cgctgaaaac catgcgcccc ccgatgggag cgatgcggac tacggactta
     5881 ctgccggcta actcgcggcg gcaggacggc cgctgtcctc cccggcccct tcgagttcca
     5941 cccaatcctg ggaccagcgc gacagagctt cgacgacctc gtttagcgcg cgccccttct
     6001 ccgtgagccg gtactcgacc cgcaccggcc gctccggcac caccacccgc tcgacgatac
     6061 cctctgcctc gaactccctc aaacgctccg agagcatccg gttcgagagg tcggggatag
     6121 tctcctgtat ctccccgaaa cgcgaagcgc ccgcgagcat cgcccgcagt atcgcaccgc
     6181 tccaccggcg acccacgatc tcgaccgccc gctgatacct gggacagaac ctctccgaat
     6241 agatctcatc cgaaccaatc atcacaaccg cattttacca cgcttagaga cttgacgtaa
     6301 ctaatatatg aaattatact tggttacgtt gagtaactaa aggcccaggg aggtcgcaga
     6361 tgttggatgt tggtttgctg gtattaaggg ttgtgctcgg tgtgatcttc gccgcccacg
     6421 gcgctcagaa gttgttcggt tctttcggtg ggccggggtt gaagggtacg gcggggttcc
     6481 atgagcagtt gggcataaag ccgccttacc tgatggcgat cctcgcggga ctcgccgagt
     6541 tcgctggcgg gatactggtg gcggctgggc tcctcacgcc gctggcggcg gtggccctga
     6601 tcgtgacgat ggccgtcgcg gtgtttgcgg tgcatctgaa gaacggcttc ttcgccatga
     6661 acggtggtta cgagttcaac ctggcgctcg ccggcatggc cctgacgctg ctgctcaccg
     6721 gcgcgggcgc catcagtctc gacgccgctc tgggcatcgt cctctggtaa cggtcgttaa
     6781 agagtaagga gagatcatga acgtaaccat catcggctcg gggaacatgg ctcgcgggat
     6841 cggcgaccgg cgagtcgccg gcgggaacag cgtccgcatc atagaccggg accccgagaa
     6901 ggcgggcgcc ctcgcgggag agttgcgggg gacggatggc
//
LOCUS       DASSMB010000290         5152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_868608, whole genome shotgun sequence.
ACCESSION   DASSMB010000290 DASSMB010000000
VERSION     DASSMB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868608"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1654
                     /gene="radA"
                     /locus_tag="VFJ72_16495"
     CDS             251..1654
                     /gene="radA"
                     /locus_tag="VFJ72_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7481106.1"
                     /translation="MATKTLYVCSNCGHQEPKWLGRCPDCGEWSTFVEEAREDKKVVG
                     FAARAAGAKAGGGRAAGSVMSLSEVPPAESEGRMDAGVGELNRVLGGGIVPGSMVLVG
                     GEPGVGKSTLLLQVMGHLGEGCLMISGEESPRQVALSARRLGVNEAGFRVLSETDVDV
                     IEATILEERPSIVVVDSIQTLYSPELSGAPGGVGQVRECAARLMRLAKSEGIAVFLVG
                     HVTKEGSIAGPRVLEHMVDTVLQFEGDRFQSFRVLRALKNRFGSTNEVGVFEMTGSGM
                     VEVEDPSAFFLSRRGEKTPPGVVTVCLLEGTRPMLVEIESLVAPSPLANPRRVANGVD
                     TGRVNMLCAVLARRAGLPLHDQDVYVNVTGGVRVEEPAADLGVALAIASAMRDRPAGT
                     GTACFGEVGLTGDVRFVSGAGRRVSELLKMGFERIIRPEAMSESPSGTESDGRKKREA
                     NVLEVGTLEEAVAVALL"
     gene            1651..2736
                     /gene="disA"
                     /locus_tag="VFJ72_16500"
     CDS             1651..2736
                     /gene="disA"
                     /locus_tag="VFJ72_16500"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HET7481107.1"
                     /translation="MSPRRRDLPAELAGALEMVAPGTEIRDAIENIIRAHNGALIVVA
                     NPEKLEQLGIISGGMKVELEFAAMRLYELAKMDGAIVVAPDLSMIHYANVQLSPDPKF
                     ESGETGMRHLAGHRTAQQAGELVIVVSERRRVVSLYQGAYGPHVLEDIGVVLSKANSA
                     IATLEKFTRRLREEARSLTIHEYDGSVTLREVVAAIGTFEYSVRIAEEIEAFVRELGQ
                     EGRLIQMQLEQAFHNVPEQYDALLKDYVAEETDYRELRAALRTFSSEQLSDTMEITQS
                     LGYGSVGQTEDFFVKPRGYRQLVRVPRLPRKVAESLIAEFGSLEGLLDATEEDLDDVE
                     GVGQARARAIQRGLRRQRELEPSREVL"
     gene            2736..3275
                     /locus_tag="VFJ72_16505"
     CDS             2736..3275
                     /locus_tag="VFJ72_16505"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET7481108.1"
                     /translation="MAGEDQSKDGVANTQSVAFSEGDLVVYPSHGAGCVAGIEQKTIL
                     GEERRYYIVYIPDAELTLSIPADGETGLRSCSDEESVEAALSALEDGATEMPSNWNHR
                     LKHNREKIRSGEIAEVAEVVRNLSVYGGEHGLSTGERNMLMKARQILISEIALVREMD
                     ISEAESLVDGALSNGGSGN"
     gene            3308..4018
                     /gene="ispD"
                     /locus_tag="VFJ72_16510"
     CDS             3308..4018
                     /gene="ispD"
                     /locus_tag="VFJ72_16510"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET7481109.1"
                     /translation="MLAGGTGSRMGRPKQFIDLLGRPALYHTLRAFEEAPGIDRIYTV
                     GDAARIGELAAMSGISKYSGCASPGESRSLSTRSGLGLCAEDPESVVLIHDGSRCLVT
                     PELIQRVIEAAHGDADGVIPTLPVSDTIKVAENGAVMETLDRSRLHATQTPQAFRLGL
                     LRRIYAASEEFLLTATDDASLVERAGGCVAIVHGDKTNIKLTSPEDLILAEAILTSRS
                     RLHNGDGAHRGILWRTRH"
     gene            4000..4512
                     /gene="ispF"
                     /locus_tag="VFJ72_16515"
     CDS             4000..4512
                     /gene="ispF"
                     /locus_tag="VFJ72_16515"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET7481110.1"
                     /translation="MEDATLSFRVGLGVDVHAFAQPEEDRKLILGGVEIPSERGLTGH
                     SDADVLAHAITDALLGAAGLEDIGHYFPDTDERYRDADSIELLRHVRELVGDSWKVVN
                     VDSVVVCERPKIRDHRDAMRRNLAVALDTDLSRVTVRGTTSERLGFTGRGEGIAAQAI
                     CLLESLREGA"
     gene            4518..>5152
                     /locus_tag="VFJ72_16520"
     CDS             4518..>5152
                     /locus_tag="VFJ72_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HET7481111.1"
                     /translation="MAEVRTRFAPSPTGMLHLGGARTALFNYLFARHHEGTILLRVED
                     TDAARSTREFETAQLEDLDWLGLSFDEGPYRQSERGDYYERAAERLSEAGLTYEAEDE
                     GGRRALYFRPPERGGSFRDELRGKVRFSRISDFVLRKSDGTPSYNFAAVVDDLEMGIS
                     HVIRGEEHLSNTGRQALLYQALGASEPTFVHLGIILGPDGKKLSKRHGDASV"
BASE COUNT          931 a         1528 c         1861 g          832 t
ORIGIN      
        1 gatggatggt cacgtcgagt tcctttgagg caaaggtctt aaacggctca aaggtcatca
       61 tcgagccgaa cgaaatctcc aacgacgacg tggccgaaga cgagaccatc gtggacatcc
      121 gggtcacgca gccgaaggaa gtcgtaaagg ccgagtaagg gaatcgggct agcaatatca
      181 gggccgaggc gcgaaagcgc tccggtcttt ctgttgctgt acttctgtag cgatctcgta
      241 caataacctt atggctacta aaactcttta cgtctgctct aactgcggac accaggagcc
      301 gaaatggctg ggacgctgcc cggactgtgg ggagtggagc acctttgtgg aggaggcgcg
      361 ggaggataag aaggtggtcg ggttcgcggc gcgggcagcc ggggcgaagg ctggcggcgg
      421 gcgagccgcc ggcagcgtga tgtcgttgag cgaggtgccg ccggccgaga gcgagggccg
      481 gatggacgcg ggagtcgggg agttgaaccg ggtgctgggc ggtgggatcg tgccgggctc
      541 tatggtgctc gtcggcggcg agccgggggt tggaaagagc acgctgctct tgcaggtgat
      601 gggccatctc ggcgaggggt gcctgatgat ctccggagag gagtcgccgc ggcaggtggc
      661 tctctcggcc cggcggctcg gagtgaacga ggccggtttc cgggtgctct cggagacgga
      721 cgtggacgtt atcgaggcga cgatcctgga ggaacggccg agtattgtcg tcgtggactc
      781 tattcagacg ctgtattcgc cggagctttc cggggcgccg gggggcgtgg ggcaggtgcg
      841 ggagtgcgcg gcgcgtctga tgcggctggc gaagtccgag gggatcgcgg tgtttctggt
      901 cgggcacgtg acgaaggaag gctccattgc cgggccgcgc gtgctggagc acatggtgga
      961 cacggtcctg cagttcgagg gcgacaggtt ccagagtttc cgggtcttgc gggcgctcaa
     1021 gaaccggttc ggttcgacta acgaggtcgg ggttttcgag atgacgggta gcgggatggt
     1081 ggaggtcgag gacccgtcgg ctttcttttt gtcgcggcgg ggtgagaaga cgccgccggg
     1141 ggtggttacg gtgtgtcttt tggaggggac gcggccgatg ctggtggaga tagagagcct
     1201 ggtcgcgccg agcccgctgg ctaacccgag gcgggtggcg aacggggtgg acacggggcg
     1261 ggtgaacatg ctgtgcgcgg tgctggcgcg gcgggcgggg cttcctttgc acgatcagga
     1321 cgtgtacgtg aacgtgacgg gcggggtgcg ggtcgaggag ccggcggcgg acctcggggt
     1381 ggcgctggct atcgcgtcgg cgatgcggga ccggccggcg ggaacgggga cggcttgttt
     1441 cggtgaggtc gggctcacgg gggacgttcg gttcgtctcc ggggcgggcc ggcgggtctc
     1501 cgaacttctc aagatgggct tcgagcgtat aataaggccg gaagctatgt ccgaaagtcc
     1561 gtccggtaca gagtccgatg ggcggaaaaa gagagaggcg aacgtgttgg aagtaggtac
     1621 gctggaagag gccgtggcgg tggcgctcct gtgagcccgc gacggcgaga cctgccggcg
     1681 gagcttgcgg gggcgctcga gatggtggct ccggggacgg agatccggga cgccatcgag
     1741 aacatcatcc gggcgcacaa cggcgcgctc atcgtggtcg ccaacccgga gaagctggag
     1801 cagttgggga tcatatccgg cggcatgaag gtcgagttgg agttcgccgc gatgcgcctc
     1861 tacgaattgg cgaagatgga cggcgcgatc gtggttgctc cggatctctc catgatccat
     1921 tacgccaacg tgcagctcag cccggacccg aagttcgagt cgggcgagac cggcatgcgc
     1981 catctggccg gacaccgcac cgcgcagcag gcgggagagt tggtgatcgt ggtctccgag
     2041 cggcggcggg tggtgagcct gtatcaggga gcttacgggc cgcacgtcct ggaggacatc
     2101 ggggtcgtgc tctccaaggc gaactccgct atcgccacgc tggagaagtt cacccggcgg
     2161 ctgcgcgagg aggcgaggag cctgaccatc cacgagtacg acggttcggt gacgctcagg
     2221 gaggtggtcg ccgccatcgg gacgttcgag tactcggtga ggatcgccga ggagatagaa
     2281 gcgttcgtgc gggagttggg ccaggagggg cggctcatac agatgcagct agagcaagcg
     2341 ttccacaatg taccggagca gtacgacgcg ctcctcaagg actacgtggc ggaggagaca
     2401 gactacaggg agttacgggc ggcgctgagg acgttctcgt ccgagcaact ctcggacact
     2461 atggagatca cgcagtccct cggttacggg tcggtggggc agacggagga cttcttcgtc
     2521 aagccgcgcg gataccggca gctcgtgcgc gtgccgcgcc tcccgcgcaa ggtggcggag
     2581 agcctgatcg cggagttcgg gtcgctcgaa gggttgctcg acgctaccga ggaagacctg
     2641 gacgacgtgg aaggcgtcgg acaggctcgc gcccgcgcga tccagcgcgg actcagacgg
     2701 cagcgggaac tcgaaccatc gagagaggta ctctagtggc aggcgaagat cagagcaagg
     2761 acggagtggc gaacacgcag tcggtagctt ttagcgaggg ggacctggtc gtgtacccga
     2821 gccacggggc ggggtgcgtc gccggtatag agcagaagac catcctcggc gaggagcgcc
     2881 gctactacat cgtctacatc ccggacgcag agcttacgct gagcattccc gcggacggcg
     2941 agacgggact caggtcctgc tcggatgagg agagcgtcga ggcggcgctc tccgcgctgg
     3001 aggacggggc gacggagatg ccgagcaact ggaaccaccg gctcaagcac aaccgggaga
     3061 agatccggtc cggcgagatc gccgaggtcg ccgaggtcgt caggaacctc tccgtctacg
     3121 gtggcgagca cggtctctcg accggcgagc gcaacatgct gatgaaggcg cgccagatcc
     3181 tgatctccga gatcgctctc gtgcgcgaga tggacatctc tgaggccgaa tctctggtgg
     3241 atggcgccct gagcaacggt gggagcggga actaatagat tccgggcggg catccacgat
     3301 cgcgctcgtg ctcgccggcg ggaccggcag ccgtatgggc cgtcccaagc agtttataga
     3361 cctgctcgga cgtcccgccc tctaccacac cctccgggcc ttcgaggaag cgccggggat
     3421 agaccgcatc tacaccgtcg gggacgcggc gcggataggc gaactggccg cgatgtccgg
     3481 catcagcaag tattccgggt gcgcctcgcc gggtgagagc cggtccctct ctaccaggag
     3541 cggtctcggg ctctgcgccg aagatccgga gagcgtcgtc ctgatccacg acggctcccg
     3601 ctgtctcgtc accccggagt tgatccagcg ggtcatagag gcggcgcacg gcgacgccga
     3661 cggcgtcata ccgacactcc ccgtctccga caccatcaag gtcgccgaga acggtgctgt
     3721 tatggagacg ctcgaccgca gcaggctcca cgccacccag accccccagg ccttccggct
     3781 cgggcttctg cgccggatct acgccgcctc cgaagagttc ctcctgaccg ccactgatga
     3841 cgcctcgctg gtcgagcgcg ccgggggttg cgtcgccata gtccacgggg acaagaccaa
     3901 tataaagctc acatcacccg aggacctgat cctggccgag gccatcctga cctcccggag
     3961 ccgcctgcac aacggcgacg gcgcccaccg cggcatcctg tggaggacgc gacactgagc
     4021 ttcagggtcg gcctgggcgt ggacgttcac gccttcgcgc agccggaaga agaccggaag
     4081 ctcatcctcg ggggcgtgga gatcccctcc gaacgcggcc taaccggtca ctccgacgcc
     4141 gacgtcctcg cccacgccat aaccgacgcc ctcctcggcg ccgccggcct cgaagacatc
     4201 ggccactact tcccggacac cgacgagcgc taccgggacg ccgactccat cgagctgctg
     4261 cgccacgtcc gagaactggt cggagactca tggaaggtcg tcaacgtgga ttcggtcgta
     4321 gtctgcgagc ggcccaagat ccgggaccac agggatgcca tgcgccggaa cctcgccgtc
     4381 gccctcgaca cggacctctc ccgcgtgacc gtccgcggca cgacgagtga acgcctcggt
     4441 ttcacgggac gcggcgaggg gatcgccgcc caggcaatct gcctcctgga gagccttcgc
     4501 gaaggtgcct gaagcccgtg gctgaggtcc gaacgcgctt cgccccgagt ccgacgggga
     4561 tgctccacct cggcggcgcc cgcaccgcgc tcttcaatta cctcttcgcc cgccatcacg
     4621 aaggtacgat actcctccgc gtcgaggaca cggacgcggc tcgcagcacg cgggagttcg
     4681 agacggcgca gctagaagac ctcgactggc tcggactctc cttcgacgaa gggccgtacc
     4741 ggcagagcga gcgcggcgac tactacgaga gagccgccga acggctctca gaggcagggc
     4801 ttacatacga ggcggaggac gagggcggac gccgggcgct gtactttcgt cctcccgaac
     4861 gcggcgggag cttccgggac gaacttcgcg gcaaggtccg tttctcccga atatccgatt
     4921 tcgtgctccg caagtccgac ggcacaccgt cctacaactt tgccgccgtg gtggacgacc
     4981 tggagatggg tatttcccat gtcatcaggg gcgaggaaca cctctccaac acgggacgac
     5041 aggcgttgct ctaccaggcg ctcggggcaa gcgagccgac gttcgtacat ctcggcatca
     5101 tccttggccc cgacggcaaa aaactcagca aaaggcatgg cgacgcgagc gt
//
LOCUS       DASSMB010000291         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1511898, whole genome shotgun sequence.
ACCESSION   DASSMB010000291 DASSMB010000000
VERSION     DASSMB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511898"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFJ72_16525"
     CDS             <1..745
                     /locus_tag="VFJ72_16525"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7481112.1"
                     /translation="ETSTGTVNDIEGFARATRDVVSIVDAVSGLGACELRTDEWGVDV
                     VVAGSQKALMTPPGLGFVSVSERAWRMHDESRMPRYYFDWTAAKKAYEKDPPQTAWTP
                     AVSVIIQLDVALQQLLDEGVENVFERHVLLGRASREGVKGMGLSLFGPDEDLNSAVTA
                     AWVPEGVDGKQFVKMLFREHGIQVAGGQGEMTGRIFRIGHCGYFDAYDIIATIAAAEL
                     ALESLGHPVELGKGVGAAQRVFSRSGAVA"
     gene            742..2310
                     /gene="serA"
                     /locus_tag="VFJ72_16530"
     CDS             742..2310
                     /gene="serA"
                     /locus_tag="VFJ72_16530"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET7481113.1"
                     /translation="MKVLIPEKLADPGIELLKQEFDVDVQLGLSPEELLEKIGEYDGL
                     IIRSATRVTAEVIERAENLKAIGRAGIGVDNIDIEAATKRGIIVANAPESNTVAAAEH
                     TLGLMLAAARRIPAADASLREGEWKRSAFKGVEVDEKTLGLIGLGHVGAIVARGAIGM
                     GMRVLAYDPYVSEDRMRSMNVERAASTDEVFEKADFVSLHVPRTPQTVGMVNEATLEK
                     MKPTAYLINVARGGIVDETALYNALKEGQIAGAALDVFVEEPTTDSPLFALPNVVVTP
                     HLGASTAEAQDRAGITAAEQVAVALRGAVPTHAINAPVPSGEGAEFVARFSGLCESLG
                     SLLYQLTDRPGDMLKIEYHGEVANYDTRLLDVSAQKGLLAPMVFEPLNFVNTPLLAKE
                     RGLRVETARSSESSDYTSLVVLRMGENVVSGTLFGPRFQPRLVEAMGFDFDVVPEKHM
                     LFIRNEDVPGMIGKVGSILGDHGINIGNMAVGRGEPGSRAAMAVTVDEPVPDDVIQVL
                     LETPGFNEARAVTL"
     gene            2311..2526
                     /locus_tag="VFJ72_16535"
     CDS             2311..2526
                     /locus_tag="VFJ72_16535"
                     /inference="COORDINATES: protein motif:HMM:NF025533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HET7481114.1"
                     /translation="MAADLDDILARYRPYGPSFKPGEKAPYSGTYLCDRGTMLPPVRI
                     QLSKGEEFPEAEGLGAHTRWRETNIQA"
     gene            2591..>3436
                     /locus_tag="VFJ72_16540"
     CDS             2591..>3436
                     /locus_tag="VFJ72_16540"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7481115.1"
                     /translation="MRRFLLFRNPVFGRFWGARIVSYLGSAATVAVLPLYVYETQGDP
                     ISVGILTLAMTLPRLLGPFAGTVSDRMDGKRLMILCDLGQAAMIGLAALLLPPFPVLV
                     LLVAGSATLSTLFFPAGRSAVPALVAPDDLTPANAFLGTAANLGFALGPALGGSAFAV
                     IGARGALAFDAVTFLVSAAFLASLPALPSAPKDGAALSAGFLAEVRDGLRYVSHQPLV
                     RALVVGLFFGITFIAVDTVALVFLARGPLETGGAGYGLLASAHGVGMILGPLLLLRRV
                     RLALVP"
BASE COUNT          530 a         1137 c         1200 g          569 t
ORIGIN      
        1 cgagacctct accgggaccg tcaacgacat cgagggcttc gcgcgggcga cgcgggacgt
       61 cgtctccatc gtggacgcgg tctccgggct cggggcgtgc gagcttcgga cggacgaatg
      121 gggcgtggac gtggtcgtcg cgggctccca gaaggcgctg atgacgcctc cggggctcgg
      181 gttcgtgagc gtgagcgagc gggcgtggcg gatgcacgac gagtcccgga tgccgcggta
      241 ctacttcgac tggacggcgg cgaagaaggc ctacgagaag gacccgccgc agacggcgtg
      301 gaccccggcg gtcagcgtga tcatccagct agacgtggcg ctccagcagc ttctggacga
      361 gggcgtcgag aacgtcttcg agcggcacgt cctgctcgga cgcgcctcgc gcgagggcgt
      421 gaagggcatg gggctctcgc tcttcgggcc ggacgaggac ctcaactcgg cggtcacggc
      481 ggcttgggtg ccggagggcg tagacggcaa gcagttcgtg aagatgctct tccgggagca
      541 cgggatacag gtcgcgggcg gccagggtga gatgaccggc aggatcttcc gcatcgggca
      601 ctgcggctac ttcgacgcct acgacatcat cgccaccatc gccgccgcgg agcttgcgct
      661 ggagtcgctc ggacacccgg tcgagctcgg caagggcgtt ggcgcggccc agagggtctt
      721 ctcccggagt ggagcggtag cttgaaggtc ctgatcccgg agaagctcgc cgaccccggt
      781 atagagcttc tcaagcagga gttcgacgtg gacgtgcagc tcggcctcag ccccgaggag
      841 ctgctggaga agataggcga gtacgacggc ctcatcatcc gcagcgccac cagggtgacg
      901 gccgaggtca tcgagcgggc cgagaacctc aaggccatcg ggcgcgcggg catcggggtg
      961 gacaacatag acatcgaggc cgcgaccaag cgcggcatca tcgtcgccaa cgcacccgag
     1021 agcaacaccg tcgccgccgc cgagcacacg ctcggcctga tgctcgccgc cgcccgccgc
     1081 atcccggccg ccgatgcttc gctgcgcgag ggcgagtgga agcggagcgc cttcaagggc
     1141 gtcgaggtgg acgagaagac gctcggccta atcgggctcg ggcacgtcgg ggcgatagtg
     1201 gctcggggtg ccatcggcat ggggatgagg gtgctcgcct acgaccccta cgtctcggaa
     1261 gaccggatgc gttcgatgaa cgtcgagcgg gccgcgtcca cggacgaggt cttcgagaag
     1321 gcggacttcg tctcgctgca cgtcccgcgc acgccgcaga cggtgggcat ggtcaacgag
     1381 gcgacgctgg agaagatgaa gccgacggct tacctgatca acgtcgcccg cggcggcatc
     1441 gtggacgaga cggcgctcta caacgcgctg aaggagggcc agatcgccgg cgccgccctc
     1501 gacgtgttcg tggaggagcc gacgaccgac tccccgctct tcgcgctccc gaacgtggtt
     1561 gttacgccgc acctcggggc gagcacggcc gaggcccagg atcgggccgg aatcacagcc
     1621 gccgagcagg tcgcggtcgc gctcaggggt gctgtcccca cgcacgccat aaacgcgccc
     1681 gtcccgtcgg gcgagggcgc ggagttcgtg gcgaggttct cggggctatg tgaatctctg
     1741 gggagcctgc tctaccagct aaccgaccgt ccgggcgaca tgctcaagat cgagtaccac
     1801 ggcgaggtcg ccaactacga cacgcgcctc ctggacgtgt cggcccagaa gggtctgctc
     1861 gcgccgatgg tcttcgagcc cctgaacttc gtgaacacgc cgctgctggc gaaggagcgg
     1921 ggtctgcgcg tcgagaccgc gcgctcgtcc gagagctcgg actacaccag cctcgtggtc
     1981 ttgcgcatgg gtgagaacgt ggtgagcggc acgctcttcg gtccacggtt ccagccgcgg
     2041 ctcgtcgagg cgatgggctt cgacttcgac gtcgtgcccg agaagcacat gctcttcatc
     2101 cgcaacgagg acgtacccgg catgatcggc aaggtcggga gcatccttgg ggatcacggt
     2161 atcaacatag gcaacatggc cgtcggccgc ggagagcccg gctcccgcgc cgcgatggcc
     2221 gtaaccgtgg acgaacccgt ccccgacgac gtcatacagg tcttgctgga gacgcccggc
     2281 ttcaatgaag caagggccgt gacgctctag atggccgcgg acctggacga catcctcgca
     2341 cgctatcgtc cctatggccc gagcttcaag cccggcgaga aagcgcccta ctccggcacc
     2401 tacctgtgcg accgggggac gatgctcccc ccggtccgca tccagctatc gaagggcgag
     2461 gagttcccgg aggccgaggg gctcggcgcg cacacccgct ggcgcgagac gaacatccag
     2521 gcgtaactcg cagctacaca agggaggtgg tggggtgcgt gatcgcggat agcgccaaac
     2581 cgcctctcta atgcgccggt tcctactctt ccgaaatccg gtgttcggac gtttctgggg
     2641 ggcccggatc gtctcgtatc tcggcagcgc cgcgaccgtc gccgtgttgc ccctctacgt
     2701 gtacgagacg cagggagatc ccatctccgt cggcatcctc acgctggcga tgacgctgcc
     2761 gcgccttctg gggccgttcg ccgggaccgt ctccgaccgc atggacggga aacggctgat
     2821 gatcctctgc gacctcggcc aagccgccat gatcggtctg gcggcgctcc tgttgccgcc
     2881 gttccccgtg ctggtgttgc tcgtcgccgg ctccgcgacg ctctccacgc tgttcttccc
     2941 cgccggccgg agcgccgtcc cggcgctcgt ggccccggat gacctgactc ccgcgaacgc
     3001 cttccttggc acagcagcaa acctgggctt cgccctggga ccggccctcg gcggttcggc
     3061 cttcgccgtg atcggggcgc ggggagcgct ggctttcgat gccgttacct tcctcgtctc
     3121 cgccgccttt ttggcaagtc ttccggcgct gccttcggct ccgaaggacg gagccgcgct
     3181 gtccgcggga tttctggccg aggttcggga tgggctgcgc tacgtgtcgc accagccgct
     3241 cgtgcgcgcg ctcgtggtgg gtttgttctt cgggataacc ttcatcgccg ttgataccgt
     3301 ggcgctggtc ttcctggcgc ggggtccgct ggagaccggc ggggcgggtt acgggttgct
     3361 ggcttcggcg cacggtgtcg gcatgatcct cggcccgctc ttacttctcc gccgcgtccg
     3421 gctggccctg gtccct
//
LOCUS       DASSMB010000292        17574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_629255, whole genome shotgun sequence.
ACCESSION   DASSMB010000292 DASSMB010000000
VERSION     DASSMB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 17574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17574
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629255"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VFJ72_16545"
     CDS             <1..267
                     /locus_tag="VFJ72_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HET7481116.1"
                     /translation="RLIKEAAATNTALELNSYVDRLDLAVPYVREAMNAGVKITVDTD
                     AHDETALSFMKYGVSQARRAWVEKESVINCMPWSDFEEYLKSGK"
     gene            complement(314..454)
                     /locus_tag="VFJ72_16550"
     CDS             complement(314..454)
                     /locus_tag="VFJ72_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481117.1"
                     /translation="MTLSGERNRLLARAAAHVYDTGRHSEMGDEPAVYELVADDSAQG
                     VS"
     gene            404..1039
                     /locus_tag="VFJ72_16555"
     CDS             404..1039
                     /locus_tag="VFJ72_16555"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7481118.1"
                     /translation="MGGGAGQQAIPLSRKGHEVTILDPSPKMLAEARRRLARENEDVC
                     RRVRLVEGTGERAREALDGETFDAALCHGVLMYLEDPRPMIHALSALVRPGGVVSVLT
                     KNAAALAARPALEGRYHDALSSLKVDRDRGRLGVVTRGDTVENLTLYFGEAGLELESW
                     YGVRVFTDHLGERMPGEDLPEVLELEWEAGRRDPYRSVARLIHLVGRKKSL"
     gene            complement(1078..1518)
                     /locus_tag="VFJ72_16560"
     CDS             complement(1078..1518)
                     /locus_tag="VFJ72_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7481119.1"
                     /translation="MSDKVILLVEDDENQVLLAMRALRQHGIVDEVDEVVIAGSGREA
                     LDYLFGTGSHSGRDTSVLPEFILLDVHLNGMDGHGVLEKLRGDERTHLVPVILFSSSN
                     EHTDVVKGYELGANSYVTKPTNFDKFSEAMQYLGWYWLNFNESP"
     gene            complement(1615..2616)
                     /gene="trxB"
                     /locus_tag="VFJ72_16565"
     CDS             complement(1615..2616)
                     /gene="trxB"
                     /locus_tag="VFJ72_16565"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET7481120.1"
                     /translation="MTGEVYDVVIVGSGPAGYTAALYAARANLNTVVFQGFESGGQLM
                     LTSDVENYPGYKDGVTGPDMMDELEAQAARFGAEMRPDNVERVDFSERPFRLWAEGED
                     EPVLAHAVVIATGAKAKWLGLESEQRLMGRGVSGCATCDGFFFREKRVAVIGGGDTAM
                     EEALFLTKYASEVLVIHRRDQFRASKIMLERAQNNPKVTFLTDTVVEEILGDDSVEGI
                     RIKNTTSGEVETLEVDGFFTAIGHEPATKLFAGQVELDAAGYILQKEHTMTSVPGVFA
                     AGDVSDTRYRQAVTAAGDGCRAAIDAERWLEEQGEAPEGAVDPGVWEAEKDRSASAL"
     gene            complement(2683..3165)
                     /locus_tag="VFJ72_16570"
     CDS             complement(2683..3165)
                     /locus_tag="VFJ72_16570"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7481121.1"
                     /translation="MELRTERKSEADVPELDAARGAAWRTLLTAHAAVVERIERELAA
                     EGVLPLGHYDVFLALYEAPDRRLRMSELARAVVLSRSGLTRLVDRLEKSGLLRRERCD
                     DDGRGCYAALTEEGLGALRRSWPVYSRGISRHFGLYLDDEEVRVLDDALGRVLVAARG
                     "
     gene            3261..3473
                     /locus_tag="VFJ72_16575"
     CDS             3261..3473
                     /locus_tag="VFJ72_16575"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET7481122.1"
                     /translation="MEEVRIAAYEDGPFLVTGAVTITDAEGNSFPVQKETVALCRCGA
                     SAGRPFCDGTHTKIGFRAAEKAVREL"
     gene            3521..3871
                     /locus_tag="VFJ72_16580"
     CDS             3521..3871
                     /locus_tag="VFJ72_16580"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7481123.1"
                     /translation="MAVKEISREGLRAKLDGGEGVVIVEALPHRYYEEAHLPGAVSIP
                     HIEVDELAPSLLPDKSARPIVYCPNRACQNSPQAARRLEALGYENVCDYEDGEQDWMA
                     AGLPIERGAVKTIG"
     gene            complement(3933..4934)
                     /locus_tag="VFJ72_16585"
     CDS             complement(3933..4934)
                     /locus_tag="VFJ72_16585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET7481124.1"
                     /translation="MSNTKWIVVLAASLVLGVLMVGLFGAPLSAQQRPKLLEACKAGG
                     DEVSARDLPSRIEPGQCPTEKVITDGGVGSALPEPGESVYVEALTTTGSQELQISHFK
                     DGTIEFNHVGGDSEEADSAEAQISLAAKGPNECRDRAHTNLSYRVESGFRYYINLGSK
                     PPELTRTATKRAIVSGGTNITRTRNKCGMGDRVPVGTSYAGRTGLSADVSSTGRCRGV
                     DRRNVVSFGSLPKGTLAVTCTYSANSPGYDKVVTSDMKINKTRANWTTNPGSRSCKRK
                     FDLESVVTHERGHTFGLGHVSESKHGNLTMSTRINGPCQASERTLGRGDVLGLDRKY"
     gene            complement(5109..6374)
                     /locus_tag="VFJ72_16590"
     CDS             complement(5109..6374)
                     /locus_tag="VFJ72_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HET7481125.1"
                     /translation="MQSESIVKDPSLAPDGHRKIDWAAEHSPVLNIVRDKYLKDGTFE
                     GLGVGVALPIEAKTAYLTVVLAEAGANVAVASPGPFFVQDDVAAALAERGVTVYASSE
                     SAPEDADRELERVIDRALDGKEAVFIDDRAGLVRLAHTTRRELLPRIRGASEETTSGV
                     SKFRAMEREGILEFPVIAANDARCKYLFDNRYGTGQSTLTAIMQNTNLMVGGKRVVVL
                     GYGWCGKGIARYAAGLGARVTVCEVDPVRGLEAYADGFDVLPSLEAAELGEVFITATG
                     SRDVLAASHFERMRDGAILANAGGVDVEIDVDGLTATAAGRCEVRRNVEEFTTADGRR
                     LHLIGRGMVVNLTAGDGHPVEIMDLTFAIQALCAYHLANNHASMENRVHLLPKEIDDE
                     VARTKLDAVGMGVDALTPEQEAFLAGWRE"
     gene            complement(6386..7246)
                     /locus_tag="VFJ72_16595"
     CDS             complement(6386..7246)
                     /locus_tag="VFJ72_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET7481126.1"
                     /translation="MKEAFTMWRHTQMVVLVALSAAIYAAVLIPFKGFAIIPGFTEIR
                     PANVFPFVFGLLFGPAGAWGAAIGNLIGDFFGTLGIGSIFGFVGNFFLGFIPYKMWGA
                     FFRRGENMEPTVNTGKKLAVYIAISILASAVCADWIAWFNDLIGFVPFAALGSIITVN
                     NAVAGIVLGPILLLVLYPRVKRWGLLWTEIMRPDEVSAARFQTLGNLLMWVGGIGALV
                     VGLAIGLGIYDQGFAAAGFAPTAGGAGGAAIGLALLPFLALIVVASFLLGGREQVEAA
                     EEEAGAPRVR"
     gene            complement(7272..8051)
                     /locus_tag="VFJ72_16600"
     CDS             complement(7272..8051)
                     /locus_tag="VFJ72_16600"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HET7481127.1"
                     /translation="MDPFLYLDRDTFVHRLDPRTKMLLLVGAFVLTFLFVNPLYQLVV
                     FAVVIVFGAIARSLVNLKRIWFILVMVGLMTVILWSIFASGETPLFWFVEREAVLYGV
                     AIALRIDITIIAGMIFLSTTRNEEIATGLVRIGIPYRFAFAISTALRLVPTIAATGST
                     IGQAQRSRGLDLESGNLIQRIRKNTPLLIPVFVSTIRSTNVFSMALESKGFGASPERT
                     YFLQLSMGRRDALVLLMFAVLLAAALALRFAGYGGLEGFSR"
     gene            complement(8052..9779)
                     /locus_tag="VFJ72_16605"
     CDS             complement(8052..9779)
                     /locus_tag="VFJ72_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET7481128.1"
                     /translation="MEQTIQPAIRIRDLSYRYRGQETLALDGVDLEVAEGEFVVVMGP
                     SGAGKSTLCASLNGLIPHFFRGRMEGEVEVRGRSTRDGKVGEFAREVGLVFQDFEAQL
                     FSTNVALEVAFGPENFQVEREEMVRRVESVLGRVRLEGFEGRQPATLSGGQKQRLAIA
                     SVLAIEPRILCLDEPTTDLDPLGKLGVFEIAEELRNRDDVTLIVVEHETEETLEADRI
                     VILREGKIIADRPAREVLRDVDLLEESRIMPLQVTRFFRQMGLWEAQLPLTPAEGIVE
                     FKRRGWRIDPERHRELVAADEAREGRYGEPLIEVEGLTHRYPNGVVALEGVDMTVRRG
                     EFLAVLGQNGSGKTTLVKHFNGLLKPSGGTVRVEGEETTRQGIRRLGQTVGYVFQNPD
                     HQIFSDTVFDEVAFGPKIRGMQESDINERVSEALAAVGLSGYEKEDPFGLTKGERQRV
                     AVASVLAVRPEVLILDEPTTGLDYAEQRSMMDLVKRLNESGSTIIAVTHTMWVVAEYA
                     HRAAVIKDGRMILSGTVREVFAKEEILRDASLRPPHIVSMGNSLGSTVLTVEELIRVT
                     EPGPKGEVE"
     gene            9987..11543
                     /locus_tag="VFJ72_16610"
     CDS             9987..11543
                     /locus_tag="VFJ72_16610"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HET7481129.1"
                     /translation="MAEFKYVGGALPAPGSQEERRRFARIQERLAPLAQRLVTDPGSP
                     QTVVVVPSLSLDAAELAKISGVHHYEERLLCMLMLLRLPRTHLVLVTSQHVPMAIVDY
                     YLHLLPGVPLRHARRRLTLLSCHDASDVPLTRKILDRPRLVERIRSAITDPFSAHLSC
                     FNSTALERSLAVRLGIPLYGNDPALNDLGTKSGGREVFREAGVPLPDGFEHLRDDGDI
                     AEAIVELKRRDPGLRRVAIKLNEGFSGEGNAVFSYDGAPENEALTRWVRDELPKRIKF
                     EANGETWERYLHKFAEMGGIVECWVEGEEIRSPSVQCRINPLGEAAVVSTHDQVLGGP
                     SGQIFLGSSFPADEAYSRDIKDAGERVGQILKERGALGRFAIDFISVKREDDGSPGWD
                     HQAIEINLRKGGTTHPYLILRFLTDGSYDADDGTYCTPTAKPCYYYASDNIQSPAYKG
                     LTPDDLVDIAVDNGLHFDSASQQGVVFHLIGALSQYGKLGAVCIGDSHESARKFYRDT
                     VEVLDRETQR"
     gene            complement(11548..12558)
                     /locus_tag="VFJ72_16615"
     CDS             complement(11548..12558)
                     /locus_tag="VFJ72_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HET7481130.1"
                     /translation="MDFGVTFQTDPPAQRVIELTQRAERLGFTHAWTFDSHILWQEPY
                     VIYSQMLSATEKIIVGPFVTNPVSRDPSVTASTFATLNDMFGNRTICGIGRGDSVVRV
                     LGRKPTTLRQLEEAMHVIKELAEGREVDYGGTSVRIPWIKDGRLDVWMAGYGPKALGL
                     IGRKADGFILQLADPVILEWTMKHVHDAAREAGRDPKDVKVCVAAPAYVGRDLAHQRD
                     QCRWFGGMVGNHVADLVSRYGEDGEVPHALTDYIKARQAYDYSHHGKAGNPTTDFVPD
                     GIVDRFCVLGSVEDHIAKLTQLRELGVDQFNVYLMHDAQESTLDAYGREIIPALESRS
                     AV"
     gene            complement(12569..13213)
                     /locus_tag="VFJ72_16620"
     CDS             complement(12569..13213)
                     /locus_tag="VFJ72_16620"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HET7481131.1"
                     /translation="MPEASTFGLFLIASLALTVTPGPAVLYIVARSVDGGLRAGLASV
                     LGVGVGGLVHIAAATIGLSALLASSAAAFSVVKWLGAAYLVWLGLQRILARDEDIATI
                     TVESERLPRVFLQAVVVDVLNPKVALFFLAFLPQFVDPSQGAAWVQILVFGAVFVAVA
                     LFTDGLYALLSSKAGGWLRRRNESPRFRRGQRYVSGGVYLALGAATAISGSGKD"
     gene            complement(13223..14752)
                     /locus_tag="VFJ72_16625"
     CDS             complement(13223..14752)
                     /locus_tag="VFJ72_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HET7481132.1"
                     /translation="MDREHYRELGVNDPAELARIKAEVSASSLYNEDLAPTGPDERTW
                     TTYNIAALWIGMAIVITTYTLASGLIAAGMNWWQALLTISLGNVIVLIPMLLNAHAGT
                     KYGVPFPVFVRASFGVRGANFAAMARALVACGWFGIQTWIGGLALDALVTAAWNGWSD
                     VAGHSFIMFFAFWAVQLVIILTGIEGVKWFESYTAPLLIAGGIALLIWGFYAGGGIGN
                     VFSTSAKLQEGNAPFWSLFWPSLAANVGYWITLSLNIPDFTRYAQSQRSQVVGQAIGL
                     PLTMTAFSFIGISVTAATLVVFKEPIWDPVALITRLASGIPLVLILAMIIIVLAQIST
                     NMAANVVSPSFDFSNLAPKYISFRTGGIITAVLGVVSFPWVLFNNLGAYIFTWLVGYG
                     SLLGAIGAVMIMDYWIVRRRTLDLADLYKYEGRYAYSNGWNWRAVIAVFIGVVPVVPG
                     FLKAATTPGFAGVFQDPTFIESLYNYGLFFTFGVAAISYLILSMLGGRSTEPAREAET
                     A"
     gene            complement(14784..16025)
                     /locus_tag="VFJ72_16630"
     CDS             complement(14784..16025)
                     /locus_tag="VFJ72_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HET7481133.1"
                     /translation="MQAEQVDARRAVEELKELAELTGGPDGARRVAWTDTWKQAREWL
                     RGKLEEIDGVEVERDEAGNLWATAPGDSEKAVLLGGHIDSVPNGGWLDGCLNTIAALE
                     VMRVLAPQQRPVTLRLVDWADEEGARFGRSLFGSGAAAGTLKPDDVRGLKDRDGVALP
                     DALREHGVDLDQVNEAGKQIENAVAYLELHIEQGPVLERMDLPLGAVLGTFGVERHVV
                     RFTGQHAHSGSTPMDVRKDAFIAAARSAIAFRDDAARRDDVRATTGFVNVSPAIVTAF
                     NGWCEMSLDQRALDADVLADMLKSAKEASERIAAEEEVTVEWEKVWEIEPIPFDDELI
                     SFAEDACNEVSGSSHRLPSGPLHDAAEMARIMPTVMLFVKSLRGLSHTKDEDTPEEDL
                     ELSVRALHRLAVRTIDWAAEK"
     gene            complement(16025..17344)
                     /locus_tag="VFJ72_16635"
     CDS             complement(16025..17344)
                     /locus_tag="VFJ72_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7481134.1"
                     /translation="MTNPDELLKRHKNVLPEWISLYYDEPIELARGEGFRVWDSEGKE
                     YLDFFGGIVTTISGHNIPEIVEAVREQAGKILHSSTLYLIENQVKLAEKLISVSPISG
                     DQKVFFVNSGSEANEAALLFATQHRRSSEVLAMRGSYHGGSFGTMGITGQSTWRPTSR
                     SALDVTYAMPPHHSYSSIYGSYAKGDPEFVRACAEDLRDLIRTATTGHVAALIAEPIQ
                     GVGGFIELPPEYIRGVKEVLDETGILYISDEVQTAFGRTGSHFWGIEESGVEPDMITM
                     AKGLGNGLAIGAVMGRAEILDSISGKLHLATFGGNHVSTAGALANLNYILDNDLQKNA
                     DEVGGYLKDRLVKMAKDSPVVGEIRGRGLMLALEMVGPDGSPDPAAAVRFLQACRERG
                     VLVGKGGIGANAIRIAPPLTITREAAETAANAFEEALAEIGTSAKVG"
     gene            complement(17341..>17574)
                     /locus_tag="VFJ72_16640"
     CDS             complement(17341..>17574)
                     /locus_tag="VFJ72_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481135.1"
                     /translation="ADIVLWDPELSITATVDNRHGNVDYTPYEGMTFHGGPASVYVRG
                     GLVYKDGEVVGERGSGRFVERRFTATRGLEVRV"
BASE COUNT         3082 a         5960 c         5453 g         3079 t
ORIGIN      
        1 cgcctcatca aagaagccgc cgccacgaac actgccctgg aactcaactc ctacgtggac
       61 cgactcgacc tcgccgtccc ctacgtccgc gaggcgatga acgccggcgt gaagatcacg
      121 gtagacaccg acgcccacga cgagacggcc ctctcgttca tgaagtacgg cgtctcccag
      181 gcgcggcggg cgtgggtcga gaaagagagc gtcatcaact gtatgccatg gagcgatttc
      241 gaggagtact tgaagagtgg gaaatagtcc cttgaaagac cccttctcaa gccttgccga
      301 gggctacatc tcctcaggag acaccctgcg ccgagtcgtc cgccacgagc tcgtagaccg
      361 cgggctcgtc gcccatctca ctgtgccggc cagtatcgta gacgtgggcg gcggcgcggg
      421 ccagcaggcg attccgctct cccgaaaggg tcacgaagtc accatcctcg atccttcacc
      481 gaagatgcta gctgaagccc gccgcaggct cgcgcgggaa aacgaggacg tttgcaggcg
      541 ggtgcggctc gtcgagggta ccggggagcg ggcgcgcgag gcgctggacg gggagacgtt
      601 cgacgcggcg ctctgtcatg gcgtacttat gtacctcgaa gacccacgtc ccatgatcca
      661 cgctctctcg gccctcgtcc gccccggcgg cgtcgtctcc gtcctcacca agaacgccgc
      721 cgctctggcc gcgcgtcccg ctctcgaagg tcgctatcac gacgccctct cctccctaaa
      781 ggttgatcga gaccgcggac gcctcggcgt ggtgacccgc ggagacaccg tcgagaacct
      841 caccctgtat ttcggggagg ccgggctcga gttggagagc tggtacgggg tgcgggtgtt
      901 cacggatcac ctcggggaga ggatgcccgg tgaggatctg cccgaggtgc ttgagctgga
      961 gtgggaggcg ggacggaggg acccttacag gtcggtggcg cgcctcatcc acctggtggg
     1021 gcgcaagaag tctctatagg tgtgacgctg gagaactccc agcaacggca gggagtctta
     1081 cggagactcg ttgaagttga gccagtacca gccgaggtac tgcatggcct cggagaactt
     1141 gtcgaagttg gtgggcttgg tgacgtagga gtttgcaccc agctcgtagc ccttgacgac
     1201 gtccgtgtgc tcgttggagg aggagaacag gatcacgggc accaggtgcg tccgctcgtc
     1261 cccgcgcagt ttttccagga cgccgtgccc gtccatcccg ttgaggtgaa cgtctagcag
     1321 gatgaactcc ggcaagacgc tggtatcgcg gccggagtgg gacccggtcc cgaagagata
     1381 atcgagcgct tccctaccgc tcccggcaat cacgacctca tccacctcat ccacgatacc
     1441 gtgctggcgc agggcgcgca tggcgaggag aacctggttc tcgtcgtcct cgacgagcag
     1501 tattacttta tcggacaatc cgtcatcacc tgaaacggca ttatacagac gaggcgccgg
     1561 gtctgaacac atcttgaccc ggcgcctcgt cagacagcgg tatctttgtc tccgctacaa
     1621 ggcggatgcg ctgcggtctt tctccgcctc ccacacgccg gggtctacgg ccccctccgg
     1681 agcctcgccc tgctcctcca gccaccgctc ggcgtctatg gccgcccggc agccgtcgcc
     1741 ggcggcggta acggcctgcc ggtagcgcgt gtccgagacg tcgcccgccg cgaagacgcc
     1801 cggcacgctg gtcatggtgt gctccttctg caggatgtag cccgccgcgt ccaactcgac
     1861 ctgtccggcg aacagcttcg tggccggctc gtggccgatg gccgtgaaga agccgtccac
     1921 ttcgagcgtc tccacctcgc cggacgtcgt gttctttatg cggatgccct ccacggagtc
     1981 atcgcccagg atttcctcta ccaccgtatc ggtgaggaag gtgaccttcg ggttgttctg
     2041 ggctctctcc agcatgatct tggacgcccg gaactggtcc cggcggtgaa tgacgagcac
     2101 ctccgaggcg tacttggtca ggaagagcgc ctcctccatc gccgtatccc cgccgccgat
     2161 gaccgcgacc cgcttctccc ggaagaagaa accgtcgcaa gtcgcgcagc cgctcacccc
     2221 gcgccccatc aggcgctgct cactctccag cccgagccac ttcgccttcg cgcccgtcgc
     2281 tataacgacg gcgtgggcca gcaccggctc atcctccccc tccgcccaaa gcctgaacgg
     2341 acgctcggag aagtccacac gctccacgtt gtccgggcgc atctcagctc cgaagcgcgc
     2401 ggcctgggcc tctagctcgt ccatcatatc cggccccgtg acgccgtcct tgtaaccggg
     2461 atagttctca acgtccgagg taagcatcaa ctgcccgccg gactcgaacc cctggaagac
     2521 gaccgtattc aggttcgccc gcgcggcgta gagagcggca gtgtaacccg ccggtcctga
     2581 acccactata acgacgtcgt agacttctcc agtcactcta aaccctcctc gaacgtgata
     2641 tccaccgttc cacgcattct agtacaaagg tctgtcttta ccttacccac gggccgccac
     2701 gagcacccgt cccagcgcat cgtccagcac ccggacttcc tcatcgtcga gatacagtcc
     2761 gaagtgcctg gagataccgc gcgagtagac cggccacgaa cgccgcaacg cccccagacc
     2821 ctcttcggtg agcgccgcgt agcacccgcg tccatcatca tcgcaccgct cgcgcctcaa
     2881 cagcccggac ttctccaatc ggtccaccag ccgcgtcaga ccgctccggc tgagtacgac
     2941 cgcccgcgcc aactcgctca tacgaagacg gcggtccgga gcctcgtata gcgccaggaa
     3001 cacgtcgtag tgcccgagcg gcaacacccc ctccgccgcg agttcgcgct ctatgcgctc
     3061 tactaccgcc gcgtgcgccg tcagaagcgt ccgccacgca gcgccgcgcg ccgcgtccaa
     3121 ctctggcacg tcggcttcgc tctttcgctc cgttcgtaac tccatcctag gattgtacca
     3181 cagatagttg cacacgcaac aacactctgc tatgcttgtt tttagttgta tacgcaacta
     3241 cttaagagag gcggtgtaat gtggaagagg tgaggatcgc ggcctacgag gacggtccgt
     3301 ttctggttac gggggcggtg acgatcacgg acgctgaggg gaacagtttc ccggtgcaaa
     3361 aggagacggt ggcgttgtgc cggtgcggcg cctcggctgg caggccgttc tgcgacggca
     3421 cgcacacgaa gatcggcttc cgggcggcgg agaaggccgt gcgcgagttg taggaaacgt
     3481 cactctagag agtagcggca cgaacgagcg aggagctagg gtggcggtaa aggagatctc
     3541 gcgggaagga ttacgggcga agctggacgg cggcgagggg gtggtcatcg tcgaggctct
     3601 gccgcacagg tactacgaag aggcgcactt gccgggggcg gtcagcatcc cccacatcga
     3661 ggtggacgaa ctcgctccga gtctcttgcc ggacaaatcg gcccggccaa tcgtctactg
     3721 cccgaaccgg gcctgccaga actcgccgca ggccgcgagg cggctggagg ctttgggata
     3781 cgagaacgtc tgcgactacg aggacggcga gcaggactgg atggcggccg gcctgccaat
     3841 cgagagagga gccgtcaaga caatcggcta gacctggaca cctgcacaag cgtcgccggg
     3901 ggctttgtgc gcgcccccgg cggctggcgt cgctagtact tcctgtcgag cccgagcacg
     3961 tcgccgcggc ccagggtcct ctccgaggcc tggcaagggc cgttgatccg agtgctcatc
     4021 gtcaggttgc cgtgtttgct ctcggatacg tggcccagcc cgaaggtgtg gccgcgctcg
     4081 tgcgtcacga cggactcgag gtcgaacttt ctcttacacg aacgcgagcc agggttcgtc
     4141 gtccagttag cgcgggtctt gttgatcttc atgtctgaag tgacgacctt gtcgtaaccg
     4201 gggctgttcg ccgagtacgt gcacgtcacg gctagagtac ccttcggaag cgaaccaaag
     4261 gatacgacgt tcctccggtc cacgccgcgg catctgccgg tcgagcttac gtcagccgag
     4321 aggccggtgc gtcccgcgta gcttgtcccc accgggaccc ggtcgcccat gccgcacttg
     4381 ttgcgggtcc tggttatgtt agtcccgcca cttacgatgg cgcgtttggt cgccgtgcgc
     4441 gtcagctcgg gcggcttgct gcccaggttg atgtagtaac ggaacccact ctctacccgg
     4501 tagctcaggt tcgtgtgggc ccggtcgcgg cactcgttcg ggcccttggc ggccagcgag
     4561 atctgggcct ccgcgctatc ggcttcttcg gaatcgccac cgacgtggtt gaactctatc
     4621 gtcccgtcct tgaagtgaga tatctgcagc tcctgggacc cggtcgtggt cagcgcctcc
     4681 acgtagacac tctctccagg ctcggggagc gcgcttccga ccccgccgtc cgttatgact
     4741 ttctcggtgg ggcattgtcc cggctctatc cgggagggca ggtcccgggc cgacacctca
     4801 tcgccgccgg ccttgcatgc ttcgagcagc ttcggacgct gctgggcgga gagcggcgct
     4861 ccgaataacc ctaccatcaa tacgcccaac acaagagacg ctgcgagtac gactatccac
     4921 ttcgtgttgg acatcaagat ccctcccgtt cgccccacca gccctccggc tatcccggca
     4981 cacgtccagc gcacccatac cagactcttt atcatcccac ccgtcggaaa actttagaca
     5041 gggtcaggtt ccgtcaggtt acgaaccggg acaccgaacc ggtatacgcg ccagaaccga
     5101 cgcctggatc actcgcgcca gcccgccagg aacgcctcct gctccggcgt cagggcgtcc
     5161 acacccatgc cgacagcgtc gagcttggtc cgggcgacct catcgtctat ctccttcggg
     5221 agcagatgaa cccggttctc catcgaggcg tggttgttgg cgaggtggta ggcgcagagg
     5281 gcctggatgg cgaaagtgag gtccatgatc tcgacgggat gcccatctcc cgccgtcagg
     5341 ttgacgacca tcccccgccc gatgaggtgc agcctccgcc cgtccgccgt cgtgaactcc
     5401 tctacgttac ggcgcacctc gcatcttccc gctgccgtcg ctgtcaatcc atcaacgtct
     5461 atctccacat ccaccccgcc agcgttcgcc aggatcgcgc cgtcccgcat ccgctcgaag
     5521 tgcgacgccg ccagcacgtc ccggctcccg gtcgccgtga tgaagacctc gcctagctcg
     5581 gcggcctcca gggaaggcag cacgtcgaaa ccgtcggcgt aggcttcgag gccgcgtaca
     5641 gggtcgacct cgcagaccgt aacgcgggca cccaggcccg ctgcgtagcg ggcgatgccc
     5701 ttcccgcacc agccgtagcc gagcaccacg acccgcttgc cgccgaccat caggttcgtg
     5761 ttctgcatga tcgccgtgag cgtcgactgc cccgtgccgt aacggttgtc gaacaggtac
     5821 ttgcagcggg catcgttcgc cgcgatgacc gggaactcaa gtatcccctc ccgctccatc
     5881 gccctgaact tcgacacccc gctcgtcgtc tcctccgaag ccccccggat gcgcggcagc
     5941 aactcccgcc gcgtcgtatg cgccagccgc acgagccccg cccggtcatc tataaagacg
     6001 gcttccttcc cgtccagcgc ccggtcaatg acccgctcaa gctcgcggtc ggcgtcctcc
     6061 ggcgcgctct cggacgaggc gtagaccgtg acgccccgct cggcgagggc ggcggccacg
     6121 tcgtcctgca cgaagaacgg tccggggctc gccacggcca cgttcgctcc agcttctgcc
     6181 agcacgaccg tgaggtaggc ggtcttcgcc tctatcggga gggcgacgcc gacgccgagg
     6241 ccctcgaaag tgccgtcctt gaggtactta tcccggacga tgttcaggac cggggagtgc
     6301 tcggcggccc agtctatctt cctgtgcccg tccggggcga gggatgggtc ctttactatg
     6361 gattcgctct gcatgacggg cctttctagc gcacgcgggg agcgccggcc tcttcctcgg
     6421 cggcctcgac ctgttcacgt ccgccgagca gaaagctggc gacgactata agggcgagga
     6481 atggcaggag agccagtcct atcgcggcgc ctccggctcc tccggcggtc ggggcgaaac
     6541 ccgcggcggc gaagccctga tcgtagatac cgagaccgat ggcgagaccc acgaccagcg
     6601 cgccgatacc gccgacccac atcagaaggt tgcccagggt ctggaacctc gcggcggaga
     6661 cctcgtcggg gcgcatgatc tcggtccaca gaaggcccca gcgcttcacc cgcgggtaga
     6721 ggacgaggag aagtatggga ccgagcacta tccccgcgac ggcgttgttg acggtgatga
     6781 tcgaaccgag ggcggcgaat gggacgaagc cgatcaggtc gttgaaccag gcgatccagt
     6841 ccgcgcagac cgccgaggcg aggatggata tggcgatgta caccgcgagc ttcttgccgg
     6901 tgttcacggt cggttccatg ttctcgccgc ggcggaagaa cgcgccccac atcttgtacg
     6961 ggatgaagcc gaggaagaag ttgccgacga acccgaagat gctgccgatc cctaaagtgc
     7021 cgaagaagtc cccgatcagg ttcccgatgg ccgcgcccca ggcgccggcc ggaccaaaca
     7081 atagcccaaa gacgaacggg aagacgttag ccggacgaat ctccgtgaag cccggaatga
     7141 tcgcgaaccc cttgaacggt atcaggaccg ccgcgtagat cgccgcgctc agggccacca
     7201 gcactaccat ctgcgtgtgc cgccacatcg taaacgcttc cttcatcacg tacctcccct
     7261 tttccgttgc cctacctgct gaacccttcc aacccaccat atcccgcgaa ccgcagcgca
     7321 agcgccgcgg ccagcaacac cgcgaacatt agcaaaacca gcgcatcccg tcgtcccatc
     7381 gagagctgga ggaaatacgt cctctccggg ctcgctccga accccttcga ctccagcgcc
     7441 atgctaaaga cgttcgtcga gcggatggtg gagacgaaga ccgggatcag gagcggagtg
     7501 ttctttctta tgcgttggat caggttcccc gactccaggt cgagcccgcg cgaacgctgc
     7561 gcctgcccga tggtcgagcc cgtcgccgct atcgtcggga cgagccgcaa ggccgtcgag
     7621 atggcgaagg cgaaacggta cggaatgccg atccttacga gcccggtcgc tatctcctcg
     7681 ttgcgggtgg tggagaggaa gatcatgccg gctatgatcg ttatgtctat ccgtagcgcg
     7741 atcgccactc cgtaaagtac cgcctcgcgc tccacgaacc agaagagcgg cgtctccccg
     7801 ctggcaaaga tggaccacag tatcaccgtc atcaagccca ccatgacgag gatgaaccag
     7861 atacgcttga ggttcacgag ggacctggct atcgccccga agacgatcac taccgcgaac
     7921 accaccagtt ggtataacgg gttcacgaat aggaacgtga gcacgaaggc ccctacgagc
     7981 agcaacatct tcgtacgggg gtcgagccgg tgtacgaacg tatcccggtc caggtacaga
     8041 aacgggtcca tctactccac ctcccccttc gggcccggtt ccgtcactct aatcaactcc
     8101 tcgaccgtga ggaccgtgga gccgagcgag ttgcccatag agactatgtg cggcggccgg
     8161 agagaggcgt cgcgcaggat ctcctccttg gcgaagacct cgcgcacggt cccggagagg
     8221 atcatgcgcc cgtcctttat taccgccgcc cggtgcgcgt actccgcgac gacccacatc
     8281 gtgtgcgtca cggcgatgat cgtggagccg gactcgttca gacgcttcac gaggtccatc
     8341 atggagcgct gctcggcgta gtcgaggccg gtggtcggct cgtccaggat caggacctcc
     8401 ggcctcaccg ccagcacgct cgccacggca acccgctggc gctcgccctt cgtcagtccg
     8461 aacgggtctt ccttctcata gccgctcagc ccgacagcgg cgagtgcctc ggagacgcgc
     8521 tcgtttatgt ctgactcctg catgccccgg atcttcggcc cgaaagccac ctcgtcgaag
     8581 acggtgtccg agaagatctg atggtccggg ttctggaaga cgtacccgac cgtctgcccg
     8641 agacgccgta tcccctgccg ggttgtctcc tccccttcca cccgcaccgt ccccccgctc
     8701 ggcttcagca gcccgttgaa gtgcttgacg agcgtggtct tcccggagcc gttctgtccc
     8761 agcaccgcga ggaactcacc ccgccgcacc gtcatgtcca cgccctccag cgccaccacg
     8821 ccgttcggat agcgatgcgt gaggccctcg acctcgatta gcggctcccc gtagcggccc
     8881 tcgcgggcct cgtccgccgc gaccagctcc cggtggcgct ccgggtctat ccgccagccc
     8941 cggcgtttga actccacgat accttctgcg ggtgtcagcg gtagctgggc ctcccacagc
     9001 cccatctgcc ggaagaagcg ggttacctgt agcggcataa tgcgagactc ttccaggagg
     9061 tccacgtcgc gcaggacctc gcgggcggga cggtcggcga ttatcttgcc ttcgcgcagg
     9121 atgacgatcc ggtcggcttc cagcgtctcc tcggtctcgt gctcgacgac gatgagcgta
     9181 acgtcgtccc ggttcctcaa ctcctcggcg atctcgaata ctcccagctt accgagggga
     9241 tccaggtccg tcgttggctc atccaggcac aggatgcgcg gctctatcgc gagcaccgag
     9301 gctatagcga gccgctgctt ctgcccgcct gagagcgttg ccggctgccg cccctcgaac
     9361 ccttcgagcc gtacccgccc cagcacgctc tcgacgcgcc ggaccatctc ctcccgctcg
     9421 acctggaagt tctccggccc gaaggcgacc tccagggcta cattagtgga aaatagctgc
     9481 gcctcgaagt cctggaagac gagaccgacc tcccgggcaa actcgccgac cttgccgtcg
     9541 cgggtcgagc ggccgcggac ttcaacttcc ccctccatcc gaccgcggaa gaagtgcggg
     9601 atcagtccgt tcagcgaagc gcagagtgtg ctcttccccg ccccagatgg tcccatcact
     9661 accacgaact cgccctcggc cacctccagg tccaccccgt ccagcgcgag cgtctcctgc
     9721 ccccggtaac ggtagctgag atcccggata cggatcgccg gttgtattgt ctgttccact
     9781 atctcgtggt cactctcatc tcacgaagca tattagataa accacatact ccgctacctc
     9841 ccgcgagtcg cgtggagtac cggagaagat gtaacatacg gagtaggata ggttggacgg
     9901 gaacgcgaag gcgggcagtc tcatcgccgc cgcgttcgcg atgggtttgc cggagggtgc
     9961 gtatgagact caccggacgt gatgcggtgg cggagtttaa gtacgtcgga ggcgctttgc
    10021 cggcacccgg ctcgcaggaa gagcggcggc gcttcgcccg gatacaggag cggctggcgc
    10081 cgctggcgca gcgtctggtc actgatccgg gtagccccca gaccgtcgtg gtggtaccga
    10141 gcctctcgct cgatgcggcg gaactggcga agatctcggg cgtccaccac tacgaggagc
    10201 gcctcctgtg catgctgatg ttgctgcgcc tgccccgcac ccatctcgtc ctggtcacca
    10261 gccagcacgt ccccatggcg atcgtggact actatctgca tctccttcca ggcgtccccc
    10321 tgcgccacgc gagacggcgg ttgacgctcc tctcctgcca cgacgcctcc gacgtgccgc
    10381 tgacacggaa gatcctggac cgtccccgtc tggtggaacg catccggtcg gctatcaccg
    10441 atcccttctc ggctcacctg agttgtttca acagcaccgc tctggagcga agcctcgccg
    10501 tgcggctggg catcccgctc tacggtaacg acccggccct caacgatctg ggcaccaaga
    10561 gcggcggccg ggaggtgttc cgggaggccg gcgtgccgct gccggacggt ttcgagcacc
    10621 tgcgcgacga tggggacatc gcggaggcca tcgtggagtt gaagcgccgc gatccgggat
    10681 tgcgccgcgt ggctatcaag ctgaacgagg gcttttcggg ggagggcaac gccgttttct
    10741 cctacgatgg cgccccggag aacgaggcgc taacacgctg ggtccgggac gagttgccga
    10801 agcgcatcaa gttcgaggcg aacggcgaga cctgggagcg ttacctccac aagttcgcgg
    10861 agatgggcgg catcgtcgag tgctgggtgg agggcgagga gatccgttct ccatcggtgc
    10921 agtgccggat aaacccgctg ggcgaggcgg ctgtcgtctc gacccacgat caggtcctcg
    10981 gcggtccctc cggacagatc ttcctgggca gttccttccc cgccgacgaa gcgtacagcc
    11041 gagacatcaa ggacgccggg gagcgagtgg gacagatact caaggagcgc ggggccctgg
    11101 gacgcttcgc tatagacttc atctccgtaa aacgcgaaga tgacggtagc cccggctggg
    11161 atcaccaggc catcgagata aacctccgta agggcggcac gacacacccg tacctgatcc
    11221 tgcgcttcct taccgacggc tcttacgacg ccgacgacgg cacctactgc acccccacgg
    11281 ccaagccctg ctactactac gcctcagaca acattcagag cccggcctac aagggcctca
    11341 cccccgacga cctcgtagac atcgccgtgg acaacggcct ccacttcgac agcgcctccc
    11401 agcagggcgt tgtcttccac ctcatcggcg ccctctccca gtacggcaag ctcggcgccg
    11461 tctgcatcgg cgacagccac gaaagcgcga ggaagttcta ccgggataca gtggaggtgc
    11521 tcgaccgcga gacgcaacga taattcgcta cacggccgac cgtgactcca gcgccggtat
    11581 gatctcccgc ccgtaagcgt ccagtgtgga ctcctgcgcg tcgtgcatga ggtagacgtt
    11641 gaactggtcc acgccgagtt ccctcagttg cgtgagcttg gctatatggt cctctacact
    11701 ccccagcacg cagaagcggt ccactatgcc gtccgggacg aagtcggtcg tggggtttcc
    11761 ggccttgccg tggtggctgt aatcgtaagc ctgccgcgcc tttatgtagt cggtgagggc
    11821 gtgcggcacc tcgccgtcct cgccgtagcg ggagacgagg tcggctacgt ggttcccgac
    11881 catgccgccg aaccaccggc actggtcgcg ctggtgcgcg aggtctctgc cgacgtaggc
    11941 cggcgcggcg acgcagacct ttacgtcctt cgggtcgcgt cccgcctccc tcgcggcgtc
    12001 gtggacgtgc ttcatggtcc actccaggat gaccgggtcg gcgagctgca ggatgaagcc
    12061 gtcggccttg cgtccgatga ggccaagcgc cttcggaccg tagccggcca tccacacgtc
    12121 tagcctgccg tcctttatcc acgggatgcg gacgctggtg ccgccgtagt cgacctcgcg
    12181 gccctcggcc agctctttta tgacgtgcat cgcttcttcc aactgccgca gcgtggtcgg
    12241 tttgcgtccg agcacccgca ccacagagtc gccgcgccca atgccgcaga tggtgcggtt
    12301 gccgaacatg tcgttgagcg tggcgaaggt cgaggcggta acggacgggt cgcggctcac
    12361 cgggttggtg acgaacgggc cgacgatgat cttctcggtc gcggagagca tctgcgagta
    12421 gatgacgtag ggctcctgcc agaggatgtg tgagtcgaag gtccaggcat gggtgaaccc
    12481 gagccgttcg gctctttgtg ttaactcaat gacacgttgg gctggcgggt cggtctggaa
    12541 ggttacgccg aagtccatgt tgcctcctct aatccttccc ggagccggag atcgcggtgg
    12601 cggcgccgag cgcgagatag acgccgccgg agacgtaacg ctggccgcgc cggaacctgg
    12661 ggctttcgtt tctccgccgc agccagccac cggccttgct cgagagtagc gcgtagagac
    12721 cgtcggtgaa cagcgcgacg gctacgaaca cggctccgaa caccaatatc tgtacccatg
    12781 ccgcgccctg cgaggggtcc acgaattgcg gcaggaaggc cagaaagaac agcgcgacct
    12841 tggggttgag cacgtccacg accacggcct gcaagaagac tcgcgggagg cgctcgctct
    12901 cgacagttat cgtggcgatg tcttcatcgc gggccaggat gcgctgcagg ccgagccaga
    12961 cgaggtaggc cgcgcccagc cacttcacca cggagaaggc ggccgcggag gatgccagaa
    13021 gcgccgatag cccgatggtc gcggcggcga tgtgtaccag gccgccaacg cccacgccca
    13081 gtacggaggc caggcccgcc cggagtcctc catccacgct gcgcgcgacg atgtagagga
    13141 ccgccgggcc gggcgttacg gtcagcgcca gggaggcgat gaggaagagc ccaaaggtgg
    13201 aggcttccgg catcggcgac cttcaggcgg tctccgcctc gcgggcgggt tcggtagaac
    13261 ggccgccgag catggacagg atcaggtacg atatcgcagc gacgccgaag gtgaagaaca
    13321 ggccgtagtt gtacaggctc tcgataaacg tcgggtcctg gaacacgccg gcgaaaccgg
    13381 gggtcgtggc ggccttcagg aagccgggga ccaccgggac cacgccgatg aagacggcta
    13441 ttacagcccg ccagttccag ccgttcgagt aagcgtatct tccctcgtac ttatagaggt
    13501 cggccaggtc tagggtgcgg cggcgaacga tccagtagtc catgatcatc acggccccga
    13561 tggcgccgag caaactgccg tatcccacga gccaggtgaa gatgtacgcc ccgaggttgt
    13621 tgaagagcac ccacgggaac gagacgacac cgaggaccgc ggttattatg ccacccgtcc
    13681 tgaacgagat gtacttcgga gcgaggttcg agaaatcgaa cgagggcgag acgacgttcg
    13741 cggccatgtt cgtcgagatc tgggccagca cgataatgat catggcgagg atcaagacca
    13801 agggtatacc gctggcgagc cgcgtgatga gcgccaccgg gtcccagatg ggctccttga
    13861 agacgacgag ggtagccgcc gtgaccgaga tacctataaa gctgaacgcc gtcatcgtga
    13921 gcgggaggcc tatggcctgg ccgacgacct gcgagcgctg gctctgggcg tagcgggtga
    13981 agtccgggat gttgagggac agcgtgatcc agtaccccac gttcgccgcc aacgacggcc
    14041 agaacagcga ccagaacggc gcgttaccct cctggagctt cgccgaggtc gagaacacgt
    14101 tcccgatccc gccgcccgcg tagaaccccc agatgagcag cgcgatgccg cccgctatga
    14161 ggagcggcgc ggtgtaggac tcgaaccact taacgccctc gatgccggtc aggatgatca
    14221 cgagttgcac cgcccagaag gcgaagaaca taataaacga gtgtccggcc acatccgacc
    14281 agccgttcca ggccgccgtc accagcgcgt cgagggcgag gccgccgatc caggtctgta
    14341 tcccgaacca tccgcaggcg acgagcgcgc gggccatcgc cgcgaagtta gcccctcgca
    14401 ccccgaacga ggcgcggacg aagaccggga acgggacgcc gtacttggtg ccggcgtggg
    14461 cgttcaggag catcgggata agcacgatca cgttacccag cgagatcgta agcagcgcct
    14521 gccaccagtt cataccggcg gcgatgagcc cggaggccag cgtataggtc gtgatgacta
    14581 tagccatccc gatccagagc gccgcgatgt tgtacgtcgt ccacgtccgc tcatcgggtc
    14641 ccgtgggcgc caggtcctcg ttgtagaggc tggaggcgct tacctccgcc tttatccgcg
    14701 ccaactccgc cggatcgttg accccaagct ccctgtagtg ttcccggtcc atcgtcctcc
    14761 ctttcgccag ctttcagcta tggttacttc tccgccgccc aatctatcgt ccgcaccgcg
    14821 agccggtgga gcgcccggac cgacaactcc aggtcctcct ccggcgtgtc ctcatccttc
    14881 gtatggctca ggccccgcaa gctcttgacg aacaacatca ccgtgggcat gatgcgcgcc
    14941 atctcggcgg cgtcatgcag gggtccgctc ggcagtcggt gcgagctgcc cgagacctcg
    15001 ttgcacgcat cctcggcgaa ggagatcaac tcgtcgtcga acgggatggg ctctatctcc
    15061 cacaccttct cccactcgac ggtaacttcc tcttccgccg cgatcctctc gctcgcctct
    15121 ttcgcgctct ttagcatgtc cgcgagcacg tcggcatcca gggcgcgctg gtcgagggac
    15181 atctcgcacc agccgttgaa cgccgtcacg atggccgggc tgacgttgac gaagccggtc
    15241 gtagcgcgca cgtcgtcgcg gcgcgcggcg tcgtcccgga acgcgatggc cgaacgggcc
    15301 gccgcgatga aggcgtcctt ccgcacgtcc atcggcgtgg agccggagtg ggcgtgctgg
    15361 ccggtgaacc ggacgacgtg ccgctccacc ccgaacgtcc cgagcaccgc gccgagcggc
    15421 aggtccatcc tctccagcac cggcccctgc tcgatgtgaa gctccagata cgcgacggcg
    15481 ttctctatct gcttccccgc ctcattcacc tggtccaggt ccaccccgtg ctcgcgcagc
    15541 gcgtccggca gcgccacgcc gtcccggtcc ttgaggccgc gcacgtcgtc gggctttagc
    15601 gtaccggcgg cagccccgga gccaaagagg ctacggccaa agcgggcgcc ctcctcgtcg
    15661 gcccagtcca cgagccgcag ggtcacgggg cgctgttgcg gcgccagcac ccgcattact
    15721 tcgagggcgg cgatggtgtt gaggcagccg tcgagccagc cgccgttggg gacggagtct
    15781 atgtggccac cgagcaggac cgccttctcg gagtcgccgg gggccgtggc ccagaggttc
    15841 ccggcctcgt cgcgctctac ctcgacaccg tctatctctt cgagcttgcc gcgcagccac
    15901 tcgcgcgcct gtttccaggt gtcggtccag gcgacgcgcc gggcgccatc gggaccgccg
    15961 gtcagttcgg ccagctcctt cagctcctcg acggcgcggc gggcgtctac ctgctcggcc
    16021 tgcatcagcc gaccttcgcc gaagtgccga tctccgctag ggcctcctcg aaggcgttgg
    16081 ccgcggtttc ggcggcctcg cgggtgatgg tgaggggcgg ggcgatgcgg atggcgttcg
    16141 cgccgatgcc gcccttgccg accaggacgc cgcgctcgcg gcaggcttgc aggaagcgga
    16201 cggcggcggc cgggtccggg ctaccgtcgg ggccgaccat ttcgagggcg agcatcaggc
    16261 cgcggccccg gatctcaccg acgaccgggc tgtctttcgc catctttacg agacggtcct
    16321 tgaggtagcc gcccacctcg tccgcgttct tctggaggtc gttgtccagg atgtagttca
    16381 ggttggcaag agcgccggcg gtggagacat ggttgccgcc gaaggtggcg aggtggagct
    16441 tgcccgagat ggagtccagt atctcggcgc ggcccatcac ggcgccgatg gcgaggccgt
    16501 taccgagacc cttcgccatc gtgatcatat ccggctccac ccccgactcc tcgatgcccc
    16561 agaaatgcga tcccgtcctg ccgaacgccg tctgcacctc gtcggagatg tacaggatgc
    16621 cggtctcgtc cagcacctcc tttacgcccc ggatgtactc cggcggcagc tctataaacc
    16681 cgccgacgcc ctggatgggt tcggctatga gggcggcgac gtgtccggtg gtggcggtgc
    16741 ggatcaggtc ccgcaggtcc tcggcgcacg ccctcacgaa ctccgggtcg cccttcgcgt
    16801 agctcccgta gatggaggag taggagtggt gcggcggcat cgcgtaggtt acgtcgaggg
    16861 ccgagcggct cgtcgggcgc cacgtactct gtccggttat ccccatcgtg ccgaaagaac
    16921 cgccgtggta cgagccgcgc atcgcaagga cctcgctgga gcggcggtgc tgagtggcga
    16981 acaggagcgc cgcctcgttc gcctccgagc cgctgttcac gaaaaagacc ttctggtcgc
    17041 cggagatcgg ggagacggag atcagcttct ccgccagctt cacctggttc tctatgaggt
    17101 agagggtaga ggagtgcagg atcttcccgg cctgctccct caccgcctcc acgatctcgg
    17161 ggatattgtg cccgctgatg gtcgtgacga tgccgccgaa gaagtccagg tactctttcc
    17221 cctccgagtc ccagaccctg aagccctcgc cgcgcgcaag ctcgatcggc tcgtcgtagt
    17281 agagagagat ccactccggc aaaacgttct tgtgtcgctt cagcagttcg tccgggttcg
    17341 tcatacccgc acctccagtc ccctcgtcgc cgtgaacctc cgctcgacaa accgcccgga
    17401 gccgcgctcg cccacgacct cgccatcttt atagaccaac ccgccccgca cgtacacgga
    17461 cgccggtccg ccgtggaacg tcatcccctc gtagggcgtg tagtccacgt tgccatgccg
    17521 gttgtccacg gtggccgtga tcgagagttc cgggtcccag agcacgatgt ccgc
//
LOCUS       DASSMB010000293         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1379104, whole genome shotgun sequence.
ACCESSION   DASSMB010000293 DASSMB010000000
VERSION     DASSMB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379104"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFJ72_16645"
     CDS             <1..804
                     /locus_tag="VFJ72_16645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HET7481136.1"
                     /translation="GTHPFSDSMEQPITGDNRYRQVEERMGWTARMQAIYGLHVHVAV
                     PDAEHAIRAVSALSRHVPLFLALSANSPFWNGSETRLASVRAKVFELVPRSGLPPAFR
                     GWEDFEGYVETLIGAGLIPDYSWCWWDARPHPRLGTVELRAPDAQTDAARTAALAALA
                     QCLVATAGEHAPENPLLTGENKWNATRYGLDARFHDFSTGESFTAREAARKLVKDLTP
                     VSQDLGCEAELEGVLEIVDHGNSAEEQRRVFERRGSLEDVAEHLVARTA"
     gene            complement(824..2152)
                     /locus_tag="VFJ72_16650"
     CDS             complement(824..2152)
                     /locus_tag="VFJ72_16650"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7481137.1"
                     /translation="MNIVVAGGGLAGLTAALRAIELGARVTLLEKGDRPGGSFLYSSG
                     YIWSYNDLPTYRREAPGGDPTLQTLILERLGPALDWLENATHPALTRETGNPLTFGAR
                     FDPERTVSALAERIEDSGSPILTGAALDGLVKDSNGGVAGLRFSSVGEEYEQAADAVV
                     LASGGFAANEELVGRYIIGGPGRMRPRAHPRSTGDGLLAALDAGALQSSGLDEFYGRN
                     LPAPPASFSPQEFVKVSQLYGRYAVAINANGQRYADEGADWSETTLTRATARQPGLHA
                     WYILDAAGLESRFRERTAGEMVETARRVGGIVLEAASLERLADRLAERGVPRGTLLHT
                     LEEYNTAVADGKGAELSPPRSGPASPVKIPPFVAVEVAPTITHTVGGLAVDEGCRVLR
                     RTDRCPLPGLYAAGVEVGGVSTGGYTSGLASALVFGGVAAESAVADGGAL"
     gene            complement(2156..2851)
                     /locus_tag="VFJ72_16655"
     CDS             complement(2156..2851)
                     /locus_tag="VFJ72_16655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7481138.1"
                     /translation="MKVKKLDHIALYMADRATAADFLTSHLGFHVVDHTDRYTLIGAG
                     GRIGKLTLFDAPEGTTPSPGVIERINIRVAGPEAAAAKLPAGTEAEPRDGGYFFTGPE
                     GLPFALVPGDGEFTDYDLEGFVVRSTEPEASARGFLDMGFAPGDGSTTVMAGEYRLHL
                     ADSAPEADGQGMLYHIGCLVDSAEEHRREAEEQGFEIQNFVEGPNTLAVFVRGPEDVS
                     VEYVEHKPTFSLT"
BASE COUNT          478 a         1029 c         1070 g          508 t
ORIGIN      
        1 gggacgcacc cgtttagcga ctcgatggag cagccgatca cgggcgacaa ccgttaccgg
       61 caggtggagg agaggatggg ctggacggcc cggatgcagg cgatctatgg cctgcacgtc
      121 cacgtcgccg tcccggacgc cgaacacgcg atccgggcgg tctccgcgct ctcgcggcat
      181 gtgcccctct tcctcgccct ctcggccaac tccccgttct ggaacggctc cgagacgcgc
      241 ctggcctcgg tgcgtgcgaa agtcttcgaa ctggtcccgc gctcgggatt acccccggcg
      301 ttccgcggct gggaagactt cgagggctac gtcgaaacac tgatcggggc cggcctcata
      361 cccgactact cctggtgctg gtgggacgcc cggccccacc ccaggctcgg aaccgtggaa
      421 ctgcgggcac cggacgccca gaccgacgcc gcccgcaccg ccgccctcgc cgccctcgcc
      481 cagtgcctcg tcgcgaccgc cggggagcac gcgccggaga acccgctact caccggcgag
      541 aacaagtgga acgccacgcg ctacggcctc gacgcccgct tccacgactt ctcgaccggc
      601 gagagcttca ccgcccgcga agcggcccgg aaactcgtaa aagatctaac gcccgtctcc
      661 caggacctcg ggtgcgaggc tgagttggaa ggcgtgcttg agatcgtgga tcatggtaac
      721 agcgccgagg agcagcgccg cgtcttcgag aggcggggtt cgctggagga cgttgcggag
      781 catcttgtag caagaacggc ctgatccccg aacgccaatc cttctataag gccccgccat
      841 ccgcgaccgc cgactccgcc gctaccccgc caaagactag agcggaagcc aggccgctcg
      901 tgtagccgcc ggtcgagacc ccgccgacct cgaccccggc ggcgtacagg ccgggaagcg
      961 gacacctgtc cgtccggcgc agcacccggc agccttcgtc caccgccaac ccgccgaccg
     1021 tgtgcgttat ggtcggcgcg acctctacgg ccacgaacgg tggtattttc acgggcgaag
     1081 cggggccgct cctcggaggc gaaagctccg cgccctttcc gtcggcgacg gctgtgttgt
     1141 actcctccag cgtgtgaagc agggtgccgc gtggaactcc gcgctccgcg agccggtccg
     1201 cgagccgctc caggctcgcc gcttcgagaa cgatgccgcc gacgcgccgg gccgtctcga
     1261 ccatctcccc ggcggtgcgt tcacgaaagc ggctctccag accggcggcg tccaggatgt
     1321 accaggcgtg gagtcccggt tggcgggcgg tggcgcgggt cagggtcgtc tcggaccagt
     1381 cggcgccctc gtcggcgtag cgctggccgt tggcgttgat cgccaccgcg tagcggccgt
     1441 agagctggga gactttgacg aactcctgcg gggagaaact ggcgggcggg gcgggcaggt
     1501 tgcggccgta gaactcgtcg aggccgctgc tttggagcgc gcccgcatcc agggccgcaa
     1561 ggagaccgtc gcccgtgctc cgaggatgcg cccgcggccg catccttccc ggaccgccga
     1621 tgatgtagcg tccgaccagc tcctcgttgg cggcgaatcc gccggacgcc aggaccacgg
     1681 cgtccgctgc ctgttcgtac tcctctccga cggacgagaa acgcagtccg gctacacctc
     1741 cattggagtc tttgacgagt ccgtccagtg ccgcgcccgt gagtatcggg ctgccggaat
     1801 cttctatgcg ctccgcgagc gcggagactg tgcgctcggg gtcgaaacgg gcgccgaagg
     1861 tgagcgggtt gcccgtctcg cgggtgagcg cggggtgggt ggcgttttcg agccagtcga
     1921 gggcggggcc gaggcgctcc aggatcaggg tttgcagggt gggatctcca cccggagcct
     1981 cgcgccggta ggtgggcagg tcgttgtagg accagatgta gccgctggag tagaggaagg
     2041 agccgccggg gcggtcgccc ttctccagca gcgtgacgcg cgcgccgagt tctatggcgc
     2101 gcagagcggc ggtgaggcca gcgagaccgc cgcctgcgac gacgatattc acctgttagg
     2161 tcagggagaa ggtgggttta tgttcgacgt actcgacgct cacgtcttcg gggccgcgga
     2221 cgaagacggc gagggtgttt gggccttcga cgaagttctg gatctcgaac ccctgctcct
     2281 cggcttcccg gcggtgctcc tccgccgaat ccaccaggca gccgatgtgg tagagcatcc
     2341 cctgcccatc cgcctccggt gcggaatctg cgaggtgcag ccggtactct ccggccatca
     2401 ccgttgtcga gccgtctccc ggcgcgaagc ccatatcgag aaagccgcgc gcggacgctt
     2461 ccggctccgt ggagcgcacg acgaagcctt ccaggtcgta gtcggtaaac tcgccgtctc
     2521 ccggaaccag ggcgaacggg agcccttcgg ggccggtaaa gaagtatcct ccgtcgcgcg
     2581 gctctgcttc tgtcccggcc ggcagtttcg cggcggcggc ttcggggccg gcgacgcgga
     2641 tgtttatgcg ctcgatcacc cccggcgagg gcgtggttcc ctccggggcg tcgaagaggg
     2701 tgagcttgcc gatacggcct cccgccccga tcagggtgta gcggtcggtg tggtccacga
     2761 cgtggaaccc gaggtgtgac gtcaggaaat ccgcggcggt ggcgcggtcg gccatgtaga
     2821 gggctatgtg atccagcttc ttaaccttca tggattctcc ctttcgcggg acgttcgact
     2881 cgtcaagaag atagcacgcc gtggcccgtt tttggcaagg agtcgtgacg caaaccaggc
     2941 cgggagagct taaactgtct ccattgatct cgacctcgga ggaagagtgg tggaacggac
     3001 ggtaatactc ggggcggcgc ggacgccgtt cggcaggttc ggcggggcgt tggcttctct
     3061 tagcgcgccg gagcttgggg gagcg
//
LOCUS       DASSMB010000294         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1740214, whole genome shotgun sequence.
ACCESSION   DASSMB010000294 DASSMB010000000
VERSION     DASSMB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1740214"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFJ72_16660"
     CDS             <1..532
                     /locus_tag="VFJ72_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HET7481139.1"
                     /translation="VSRIQRSTELRQERIELDKKAQKFIEEASKGEIHIVAHRRRIGD
                     DPEEYAAKEKEQREDNHIPDGEPIIFLEVEVEDASEFEDVLEVTGVEIGGHEVLRAQS
                     SVVPNAIAAFLLHVRDMTGKTPHCYFGWTEGNPIVYLFRYILFGEGDTAPVTHEVLRE
                     AEPEAERRPAIHVGGR"
     gene            529..738
                     /locus_tag="VFJ72_16665"
     CDS             529..738
                     /locus_tag="VFJ72_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481140.1"
                     /translation="MSNSRSFDDVARKQCVDLEVVRKLEITYDRTKPGKVELAVEYVG
                     GRMRVLEVDRYLIERLLERIEDQEA"
     gene            832..1614
                     /locus_tag="VFJ72_16670"
     CDS             832..1614
                     /locus_tag="VFJ72_16670"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7481141.1"
                     /translation="MANTNPLREIWNAGRTAFGLWAVVPGSFGAEVLGRAGPDYVCVD
                     QQHGLIGYDAMLPMLQAAGAAGAAPITRVLSNDSYDIMKSLDAGAWGVIVPLVNSAEE
                     AARAVAACRYPPRGIRSYGPIRASGVVGSGDPEDLDREALCFVMVETREALERVDEIA
                     ATPGLDGIYVGPADLALSLGLAPTLQVTEKEHVNAVRRILETCHRRGIAAGIHSPSGE
                     WARRHAEAGFDLVTVATDASLLAGAARAELEEARARSAAGGP"
     gene            complement(1684..1821)
                     /locus_tag="VFJ72_16675"
     CDS             complement(1684..1821)
                     /locus_tag="VFJ72_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481142.1"
                     /translation="MAKLEREVKRVLKSKSGSKKGKKGKRSSTGRSGASLEKEIKKLL
                     K"
     gene            complement(2021..3490)
                     /locus_tag="VFJ72_16680"
     CDS             complement(2021..3490)
                     /locus_tag="VFJ72_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7481143.1"
                     /translation="MAEATERRGELATELPSKHLPGVQYRDMPEPLPLGKIVGASVVL
                     LATALGSGELIIWPYITSQVGIGLLWLAFVGFSMQYFLNMEIERYTLATGETAVTGFT
                     RFWKPWGIIFCLGAFLPNMWPGWATGGATMISYLFGFETEGTKTVAILAIIGLVAIGV
                     AITVSPVIYQTIEKVQFFLVGFIALFMVVALVFATKASSWAAMVTQAPSGVANFPSLL
                     GELGIATLLGAIAFAGAGGANNLTQSNYIRDKGMGMGIHIPRIVSPITGEEVAAPSLG
                     YMTKTDEPNVRRWKGWWSVANKEQIVTFYIVGLITLIGLSVLVHSTGAKGEAADPSFL
                     QTEAQVLGRQIGYWFEVFFYLAGFAILFSTNLGIIDYVSRLIADSLKVSFFSRSEAVT
                     ESRIYFTVAWLMIIFGSIVLLAGFDQPLVLLIIASSGGGVVMAFYSWMLIVLNRRALP
                     APIRLKGWRLPIMYVIAVFFSIFSVLLVYDVVRTNVFGG"
     gene            complement(3549..4355)
                     /locus_tag="VFJ72_16685"
     CDS             complement(3549..4355)
                     /locus_tag="VFJ72_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7481144.1"
                     /translation="MSVPAGSAKNLERAFAMLRVLSQHAEGLSLAEICRRSEVSKPTG
                     HRLLGVLGEAGMVREFGRGVYGLGPQCLVLGSAFLDGLDLRREAKGVLEDLVSKTGET
                     CHLGIRDGDRIVYIEKLDSPKLVQLKTRVGLTNPLHSTALGKVLLAYDGEADIEAMFA
                     RGVERRTPNTITDVEVFRAELEEVRERGFAVDDVENEEGIRCVAAPVFDHGGAAVASI
                     SVSSPEYRVSRERLQEIGALTREAALVLSRRMGYRHGANEIGEEARKDIV"
     gene            4548..>4811
                     /locus_tag="VFJ72_16690"
     CDS             4548..>4811
                     /locus_tag="VFJ72_16690"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7481145.1"
                     /translation="MTESTGETGQGLDRLRGKSAVVTGAAKGIGRATAELFAKEGARL
                     VVTDVDEAGLQGLKEKLEGSGAEVEAVVGDVSVVEDARRMISAA"
BASE COUNT          890 a         1528 c         1556 g          837 t
ORIGIN      
        1 cgtctcgcgg atacagcgct ccacggagtt gcgccaggag aggatagagc tggacaaaaa
       61 ggcccagaag ttcatcgagg aggcgagcaa gggtgagatc cacatagtcg cccaccgacg
      121 caggatcggc gacgacccgg aggagtacgc cgcaaaggag aaggagcagc gcgaggacaa
      181 ccacatcccg gacggcgaac caatcatctt cctcgaagtg gaggtcgagg acgcctcgga
      241 gttcgaggac gttctggagg tgacgggcgt ggagatcggg ggtcacgagg tcctgcgcgc
      301 ccagagctcc gtcgtcccta acgccatagc cgctttcttg ctccatgtcc gcgacatgac
      361 cggcaagacg ccgcactgct acttcggctg gacggagggc aaccccatcg tctacctgtt
      421 ccgctacatc ctcttcggcg agggcgacac cgctccggtc acccacgagg tcctgcgcga
      481 agccgaaccc gaggcagagc gccgtcccgc catccacgtc ggcggacgat gagtaactcc
      541 cgctcgttcg acgatgtagc tcgcaagcag tgtgtagacc tcgaagtcgt ccgcaagctg
      601 gagatcacct acgatcgcac gaagccgggg aaggtagagc ttgccgtcga gtacgttggt
      661 ggtcggatga gggtgcttga ggtagacagg tacttgatcg agcgtctgct ggaacggata
      721 gaagaccagg aggcttgacc ctcgcacgag cccgcaacgc tcgacgcgga agggagcgac
      781 gcgccggacg ggttgccgga ccgcgaccgg ctttcgtgct agattggtcc ggtggcgaat
      841 accaaccccc taagagagat ctggaacgcg ggccggacgg cgttcgggtt gtgggcggtc
      901 gtgccggggt ctttcggggc ggaggtcctg ggccgcgcgg gcccggacta cgtgtgcgtg
      961 gaccagcagc acgggctcat cggctacgac gctatgctgc cgatgctcca ggccgccgga
     1021 gcggcgggcg cggccccgat cacgcgggtg ctctcaaacg actcgtacga cattatgaag
     1081 tccctggacg ctggggcgtg gggcgtgatc gtgccgctcg tcaacagcgc cgaagaagcc
     1141 gcgcgggccg tcgccgcctg ccgctacccg ccgcggggca tccgctccta cggacccatc
     1201 cgggcgtccg gcgtggtcgg ctccggcgac ccggaagacc tcgaccgcga ggcgctgtgc
     1261 ttcgtcatgg tcgagacgcg cgaagccctc gaaagggttg acgagatcgc cgcgactcct
     1321 ggcctcgacg gtatctacgt aggaccggct gatcttgctc tctcactcgg cctcgctccg
     1381 acgttgcagg tcacagagaa agagcacgta aacgccgtgc ggcgcatcct ggaaacctgc
     1441 caccggcgcg gcatcgccgc cggcatacac tccccttcgg gagagtgggc gcggcggcac
     1501 gcggaggccg gcttcgatct ggtaaccgtg gcgacggatg cgagcctgct cgcgggagcc
     1561 gcccgcgccg aactcgaaga agcccgcgcc cggagcgcgg cgggcggccc gtaagagacc
     1621 tcgacgcgcg aaaaaggccg gagcgtcaga gctccggcct cgataagcgt gtttgttctg
     1681 ggtttacttg aggagcttct tgatctcttt ctccaggctc gcgccgctcc tgccggtact
     1741 gctcctcttg cctttcttac ccttcttgct gccagacttg ctcttgagga ctctctttac
     1801 ttctctctcc agtttagcca cgtttggcgg gcctcctttg tcgtacagtg ttttgatcta
     1861 gatgttgcta cccctggacc tgcgccttca aaccgcctgg tagtagagcc ggggttgccc
     1921 tattcgagca accccagcag gtaggctatg gtggtcagga cgcaaaatac cgccaggccc
     1981 acgaggatac gaagccagag ctgtttcagc acctgggcgg ctatcctccg aagacgttcg
     2041 tgcggaccac gtcgtagacg agtagtacag agaagatgct gaagaacacg gcgatcacgt
     2101 acatgatcgg cagccgccag cccttgagcc ttatcggcgc cgggagggcc cggcggttca
     2161 gcactataag catccacgag tagaacgcca tgacgacgcc gccgcccgag gaggcgatga
     2221 tcaggagcac cagcggctgg tcaaagcccg cgagcagcac gatggagccg aagatgatca
     2281 tgagccacgc caccgtgaag tagatgcggc tctccgttac ggcctcgcta cgggagaaga
     2341 agctgacctt caacgagtcc gcgatgaggc ggctgacata gtcgatgatc ccgaggttgg
     2401 tcgagaagag gatggcaaac ccggccaggt agaagaagac ctcgaaccag taccctatct
     2461 gccggccgag cacctgggcc tccgtctgaa ggaacgaggg gtctgccgcc tcgcccttcg
     2521 cgccggtgga gtgaacgagc accgagagcc cgatgagggt tatgagcccg acgatgtaga
     2581 aggttacgat ctgttccttg ttggcgacgc tccaccagcc cttccagcgg cgcacgttcg
     2641 gctcgtcggt cttggtcatg tagccgagag acggcgcggc gacctcttcg ccggtgatgg
     2701 gggagacgat cctcgggatg tgtatcccca tgcccatgcc cttgtcgcgt atgtagttac
     2761 tctgggtgag gttgttggcg ccgcccgcgc ccgcgaaggc gatggctccg agaagcgtcg
     2821 cgatgcctag ctccccgagg aggctcggga aattggctac gcccgaaggc gcctgcgtaa
     2881 ccatcgccgc ccaggacgag gctttcgtgg cgaagaccag cgccacgacc atgaagaggg
     2941 cgataaaccc gacgaggaag aactgcacct tctcgatggt ctggtagatg accggcgaga
     3001 cggttatcgc gacgccgatg gcgacgaggc cgatgatcgc gagtatggct acggtcttgg
     3061 tgccctcggt ctcgaagccg aacaggtagg agatcatggt cgccccgccc gtcgcccagc
     3121 cgggccacat gttgggcaag aacgccccca ggcagaagat gatcccccac ggcttccaga
     3181 agcgggtgaa gcctgttacc gccgtctcgc cggtcgccag agtgtaacgc tcgatctcca
     3241 tgtttaggaa gtactgcatc gagaagccca cgaacgcgag ccagagaagc ccgatcccca
     3301 cctgcgatgt gatgtatggc cagatgatca actcccccga tccgagggcc gtggcgagca
     3361 agacaacgct cgcgcccacg atcttcccga gtggcaaggg ctcgggcatg tcccggtact
     3421 ggacccccgg taaatgcttg cttggtagct cggtggcgag ttccccccgt cgctccgttg
     3481 cctcggccat gcttattctc ctccctctct atccttcacg gtatcccaac ctgagacttc
     3541 aaattcttct aaacgatatc tttcctggcc tcctccccga tctcgttcgc gccgtggcgg
     3601 tagcccatcc tccgcgatag aaccagcgcc gcctcgcggg tcagagcccc gatctcctgt
     3661 agcctctcgc gggatacccg gtactccggg ctggagacgc tgatgctggc gaccgccgcg
     3721 cccccgtggt cgaagaccgg cgccgcgacg caccttattc cttcctcgtt ctcgacgtcg
     3781 tccaccgcga acccgcgctc gcgcacctct tccaactcgg ctctgaagac ctcgacatcg
     3841 gttatggtgt tcggcgtcct gcgctccacg ccccgcgcga acatcgcctc gatgtctgcc
     3901 tccccgtcgt atgccagcag gactttgccg agcgccgtgg agtggagcgg gttggtcagc
     3961 cctacccgtg tctttagctg caccagcttg gggctgtcca gcttctcgat gtagacgatg
     4021 cggtcgccgt cccggatgcc gaggtgacag gtctctccgg tcttcgatac caggtcctcc
     4081 aggaccccct tcgcctcccg ccggagatcc aacccatcca gaaacgcgct ccccaggacg
     4141 agacactgcg gcccgagacc atacaccccc cggccaaact ccctgaccat gcccgcctcc
     4201 ccgagcaccc cgagcagccg gtggccggtc ggcttggaga cctcgctccg gcggcagatc
     4261 tccgccagcg atagcccctc cgcatgctgc gagagcaccc gcaacatggc gaacgctctc
     4321 tccaggttct tcgccgaacc tgccggtacg ctcacaggtt agatttgaaa tagcgttcca
     4381 tttccccgcg gccagtatac ggaaaggtgc gctcgttagt caaggcaagc ggggacttgc
     4441 gtctggagcg gcctggaggc atgagcggga gttcggccct ctatggtagg ctggagcatg
     4501 gaaagtaggg gtagatacaa gggaaggcgt agaccgaagg aggcggcgtg acggagagca
     4561 cgggagagac ggggcagggc ctggaccggc tgcgcgggaa gtccgcggtc gttacagggg
     4621 cggccaaagg tatcggccgg gcgaccgccg agctcttcgc gaaagaggga gcgcggctcg
     4681 tcgtgacgga cgttgacgag gccgggctcc aggggcttaa agagaagttg gagggttcgg
     4741 gggctgaggt ggaggcggtc gtcggggacg tctcggtggt cgaggacgcg aggaggatga
     4801 tctctgcggc c
//
LOCUS       DASSMB010000295         4700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_731732, whole genome shotgun sequence.
ACCESSION   DASSMB010000295 DASSMB010000000
VERSION     DASSMB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731732"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..815
                     /locus_tag="VFJ72_16695"
     CDS             <1..815
                     /locus_tag="VFJ72_16695"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET7481146.1"
                     /translation="TLGQLIITGSSVADFGAVILLSLFFSGDSSSVGSTLLLIGAFVV
                     LVAAIGIALAEIEHLERLSMVLQKLQDSSAQIRVRGALLFLISFAVLAQFLGLEVILG
                     AFIAGTVLRLVDKDRMMTHPFFRQKLEAVGFGVFIPFFFVTSGIQLDVGALLAGGGAA
                     LASVPLFLLALLLARGLPAILYRPTVGTRGVLAAGLLQATSLPFIVATTGIGMELGIL
                     APATGAALVVAGLLSVVLFPLGALTLLRAGKKPSADSQGGLSGDAGPEPGPA"
     gene            863..1906
                     /locus_tag="VFJ72_16700"
     CDS             863..1906
                     /locus_tag="VFJ72_16700"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7481147.1"
                     /translation="MTQTHVPTDRGARLGRLRELMERLKLEALLLRRSANFAWYTGGA
                     DSRVDHASPFGVADVLVTLEAEYIFTNNIEAARMREEQTPDFEVIEHPWHEDETAAIR
                     EVVGSVSLGADFPLENALDVSGEVSPLRYVLDPDAIERYREVGADTAAAMAEAAGSVE
                     PGMTEYEAAANLAAACRKRGLFSSVLLSAADNRIASYRHPIPHGDEIERRVMLVVSAE
                     RGGLYANVTRIVDFEEPDPKIAWRQEACNSILRRMREEATHPGHTLAEAFADCRRFYA
                     EAGYPEEWKLHHQGGMTGYGSREVIANPQTQQEIQIGQAFAWNPSITGAKAEETFILT
                     ESGPEIITRLPKR"
     gene            complement(1927..2796)
                     /gene="folD"
                     /locus_tag="VFJ72_16705"
     CDS             complement(1927..2796)
                     /gene="folD"
                     /locus_tag="VFJ72_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HET7481148.1"
                     /translation="MAEPEKILDGKAVAAEIRAEVAEGVRNLAERGVPVRLDVILVGD
                     DPASTTYVNMKQRDCAEVGIESRAHRFGADVSQKELAVLVENLNDDPAVSGFFIQLPL
                     PNGLDHVPLISSIVPSKDVDGLSPMSAGRLAVGLPSLLPCTPHGVIQLLKRNGIALEG
                     KEAVVIGRSNLVGKPLAQLLLRENATVTVCHSRTKNLPDVARRADVLIVAAGRREMIG
                     AEYVKDGAAVVDVGIHRKEEGGLTGDVKFAEVEPVASRITPVPGGVGPMTRAMLLYNT
                     LEAARAGADIAQG"
     gene            complement(2800..4422)
                     /locus_tag="VFJ72_16710"
     CDS             complement(2800..4422)
                     /locus_tag="VFJ72_16710"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011443.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HET7481149.1"
                     /translation="MPHVYVTGHKNPDTDTIASAIGYAELKNLVDPDNHYAPARLGEV
                     NAQTAWALKESGAEEPKFLEHIMLRVKDAMSPEIAVVHKYDPLRNVGLVMSKRNVSQV
                     PIVDDDGSLIGLITERNLARMYIRESREPSTFADTPVSVSAIVEVLEGELVAGKEREA
                     NGKLWVISRSTDSMGQQIQDGDVVVIADRPKGQRIAIELGASVLVISNGVRPDKEVLE
                     LAEEKGTAVVLSQLDSYVTSRMIQLAVPCWEVMSEGPLTVSPDDLISDITQQVMEVHY
                     RAAIAVDDDNVPIGVVTRTDLLNAKPRRVLLVDHAEVGQSVKGVEKAQIVEILDHHHI
                     GNIETSTPIAATFDPVGSTATLVVERFKTAGLAPSESTAKMLLAALLSDTVILNSPTT
                     TERDREVARYLEELLDLSAEEFGMEMFEASSDVSELSAEEIVNRDAKEYGLGSGETMC
                     ISQVETVGKGLLDRKDELIEMLETMRRRNDYLLAALMVTDIIEGGTELLCAGDCSMVE
                     QAFEARAKDGVIDLPGVMSRKKQVAPKLLAVL"
     gene            complement(4505..>4700)
                     /locus_tag="VFJ72_16715"
     CDS             complement(4505..>4700)
                     /locus_tag="VFJ72_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481150.1"
                     /translation="ALPKRGSSSPEPASDSPSFRAGEKVRHAKFGEGEILESGAGKVV
                     VRFGSDVKTLVPELARLSKV"
BASE COUNT          757 a         1600 c         1475 g          868 t
ORIGIN      
        1 ccacgctggg acagttgatc atcaccggct cgtccgtcgc ggatttcggg gcggtgatcc
       61 tgctctcgct attcttctcc ggggactcca gcagcgtcgg gtccacgctc cttttgatcg
      121 gggcgttcgt cgtgctggtg gccgccatcg ggatcgcgct agccgagatc gaacacctgg
      181 aacgtctgag catggtcctg caaaagctcc aggactccag cgcccagatc cgggtgcgcg
      241 gcgccctcct cttcctgatc agcttcgccg ttctcgctca gttcctgggc ctggaggtca
      301 ttttgggcgc atttatcgcg ggtacggtat tgaggctcgt ggacaaagac aggatgatga
      361 cgcacccgtt cttccgccag aagctcgaag ccgtcggctt cggcgtcttc atcccgttct
      421 tcttcgtcac cagcgggata cagctcgacg ttggagcgct gctggccggc ggcggggcgg
      481 cactcgcatc cgtgccgctg ttcctgctcg ccctgctgct cgcccgcggt cttccggcga
      541 tcctctacag gccgacggtc ggcaccaggg gcgtgctggc ggcggggttg ctacaggcga
      601 cctcgctgcc gttcatcgtc gccacgacgg ggatcgggat ggagcttggc atcctcgccc
      661 ccgcgaccgg cgccgcgctt gtagtggccg ggctactgtc ggtggtgctg ttcccgctcg
      721 gcgccctcac gctgctgcgg gccggcaaga agccgtccgc ggacagccag ggaggtctct
      781 ccggcgacgc cgggccagaa cccggcccgg cctgacacga aaccgcatcg agcttgtcca
      841 tattcggaat cgaggtatcg agatgaccca gacacacgtc ccgacggatc ggggagcccg
      901 gcttgggagg ctcagggagt tgatggaacg tctgaagctg gaggcgctcc tgctgcggcg
      961 ctccgcgaac ttcgcctggt acacgggcgg agcggatagc cgggtggatc acgcctcccc
     1021 attcggcgtc gcggacgtgc tcgtcaccct tgaggcagag tacatcttca ccaacaacat
     1081 cgaagcggca cggatgcgcg aggagcagac gccggacttc gaggtgatcg aacacccctg
     1141 gcacgaagac gagaccgccg ctatccgtga ggtcgtaggt agcgtctctc tgggagcgga
     1201 ctttccgctg gagaacgctc tggatgtctc cggcgaggtc tcgcccctgc ggtacgtcct
     1261 cgacccggat gccatcgaac gctacagaga ggtcggagcc gacaccgcgg cggcgatggc
     1321 ggaggcggcg ggctccgtgg aacccggcat gacagagtac gaggcggcgg cgaacctggc
     1381 ggcagcctgc cggaagcgcg gactattctc ctccgtgctg ctgtctgccg ccgataaccg
     1441 catcgccagt taccgccatc ccataccgca cggagatgag atcgagcgcc gcgtcatgct
     1501 cgtggttagc gcggagcgcg gcggtctcta cgccaacgta acccggatcg tcgatttcga
     1561 ggaaccggac ccgaagatcg cctggcggca ggaagcctgt aactccatcc tacggcggat
     1621 gagagaagag gccacgcatc ccggacacac cctggcggaa gccttcgccg actgccggcg
     1681 tttctatgcg gaggcgggat atccggagga gtggaagctc caccaccagg gcggcatgac
     1741 gggctatgga tcacgcgagg tcatagcgaa cccgcagacg cagcaggaga tacagatagg
     1801 acaggcgttc gcctggaacc cttccatcac cggcgccaag gcagaggaga ccttcatcct
     1861 tacggagtcc gggccggaga tcataacccg gcttcccaag agatagaaag attcccgagc
     1921 aagaccctag ccctgagcga tatctgctcc cgcccgcgcc gcctccagcg tgttgtacag
     1981 gagcatcgcc cgcgtcatcg gaccgacgcc gccggggacc ggcgtgatgc gcgacgccac
     2041 cggctcgacc tccgcgaact tcacatcgcc agtgaggccg ccctcttctt tgcggtggat
     2101 tccgacatcc acgacggctg ctccatcctt tacgtactcc gcgccgatca tctcccggcg
     2161 tccggcggcg acgatcagga cgtccgcccg ccgcgccacg tccggcaggt tcttcgtgcg
     2221 cgagtggcag acggttacgg tcgcgttctc gcgcaggagg agttgggcga gcggcttgcc
     2281 gacgaggttc gagcggccga tgacaacagc ttccttgcct tctagcgcga tcccgttgcg
     2341 cttgagcagt tggatcacgc cgtgcggggt gcagggcagg aggctcggca gcccgaccgc
     2401 gagcctcccg gcgctcatcg ggctgagtcc gtccacgtct ttcgacggta cgatgctgga
     2461 gatgagcggc acgtggtcca gtccgttggg aagcgggagc tggatgaaga acccggagac
     2521 ggcggggtcg tcgttcaggt tctcgacgag gaccgccagc tctttctggg agacgtccgc
     2581 gccgaagcgg tgggcgcggg actcgatgcc gacctcggcg cagtcgcgct gcttcatgtt
     2641 cacgtaggtc gtggaggccg ggtcgtcgcc gaccaggatc acatccagcc ggaccggcac
     2701 gccccgctcc gcgaggttcc gcacgccttc ggccacttcg gcccggatct cggccgcgac
     2761 ggccttgccg tcgagtatct tctcgggctc cgccacggcc tagagtaccg cgaggagctt
     2821 gggggcgacc tgcttcttgc ggctcatcac gccgggcagg tctatgacgc cgtccttcgc
     2881 ccgcgcctcg aacgcctgct cgaccatgga gcagtcgccc gcgcatagta gctcggtgcc
     2941 gccctcgatg atgtccgtca ccatcagcgc ggcgagcagg tagtcgttcc tccggcgcat
     3001 ggtctcgagc atctcgatca gctcgtcctt gcggtccagc aagcccttcc cgacggtctc
     3061 gacctgcgag atgcacatgg tctcgccgga gcccaggccg tactccttgg cgtcgcggtt
     3121 tacgatctcc tcggcagaga gctccgagac gtccgaggag gcctcgaaca tctccatccc
     3181 aaactcctcg gcgctgaggt ccagaagctc ctccaggtag cgggcgacct ccctgtcgcg
     3241 ctcggtggtg gtcggagagt tgaggatcac ggtgtccgag agtagcgccg cgagcagcat
     3301 cttggccgtc gactcggagg gcgcaagtcc cgccgtcttg aaccgctcca ccacgagcgt
     3361 cgcggtcgag ccgacggggt cgaaggtcgc ggctatgggg gtgctggtct cgatgttgcc
     3421 gatatggtgg tggtccagga tctcgacgat ctgggccttc tccacgccct tgacgctctg
     3481 cccgacctcg gcgtggtcca cgagcaggac gcgccggggc ttggcgttca gcaggtccgt
     3541 ccgcgtcacg acgccgatgg ggacgttgtc atcgtccacc gctatcgccg cgcggtagtg
     3601 gacctccatc acctgctggg ttatgtccga gatcaggtcg tccgggctga cggtcagcgg
     3661 tccctcgctc attacctccc agcaaggtac ggcgagctgg atcatgcggc tcgtcacgta
     3721 ggaatcgagc tgcgagagca ccaccgccgt acccttctcc tcggcgagct ccaggacctc
     3781 tttatccggc cggacgccgt tcgagattac gagcaccgac gctccgagct ctatcgctat
     3841 ccgctggccc tttggacggt cggcgatgac caccacgtct ccatcctgta tctgctggcc
     3901 catcgagtcc gtgctgcgcg agatgaccca cagctttccg ttggcctccc tttccttccc
     3961 ggccaccagc tcgccttcga gcacctcgac gatagcggag acggagaccg gagtgtcggc
     4021 gaacgtggaa ggctcgcgag actcgcggat atacatacgc gccaggttcc gctcggtgat
     4081 cagaccgatc agcgatccgt cgtcatcgac tatcgggacc tgactcacgt tcctcttgga
     4141 catcaccagg ccgacgttcc gcaggggatc gtacttgtga accaccgcga tctccgggct
     4201 catcgcgtcc tttacccgca gcatgatgtg ctccaggaac ttcggctcct ccgccccact
     4261 ctccttcaac gcccacgccg tctgcgcgtt gacctccccc agccgggccg gagcgtaatg
     4321 attgtccggg tcgaccaggt tcttcaactc cgcgtacccg atggccgatg cgatggtgtc
     4381 cgtgtccggg ttcttgtgtc ctgttacgta aacgtgcggc acccgcgcct cctctagact
     4441 ctccgaaacc cttttgacgc taccctctca cccatactgc ccccatattc tacccggata
     4501 accgtcaaac tttgctcaac cgcgcaagct ccggcacgag ggtctttacg tccgacccga
     4561 aacgaacgac gaccttcccg gccccggact ccaggatctc accctccccg aacttggcgt
     4621 gccgcacctt ctccccggcc ctgaacgagg gcgaatccga cgcaggctcc gggctgctgc
     4681 tcccccgttt cggcagcgcg
//
LOCUS       DASSMB010000296        21459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1124629, whole genome shotgun sequence.
ACCESSION   DASSMB010000296 DASSMB010000000
VERSION     DASSMB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 21459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21459
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124629"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..946
                     /locus_tag="VFJ72_16720"
     CDS             110..946
                     /locus_tag="VFJ72_16720"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET7481151.1"
                     /translation="MLGSFLVMLREGFEASLPVAIVLAYLTQTGRKEDARGVWYGVGA
                     AVLISIVTAGVLFVTANGLEGTAGIYFKSGAMWLAVAFLTYMVLWMRRQARTMAQGIR
                     RNVDSAVERGGLLALGMLVFVMVLREGIETGLFMFGITQNATPLGVGIGAVAGTAAAV
                     GLGYAVYVGGKRINMGAFFKITGILLIVVAAGLLARGVAIMEIGGIIPAFFYPVWDLS
                     SIHLLSSGSVSGQFLTAFLGWDAKPDLLEVGVWAVYLLTVGFFFLRPQTRTAAQPAAA
                     HN"
     gene            985..1611
                     /locus_tag="VFJ72_16725"
     CDS             985..1611
                     /locus_tag="VFJ72_16725"
                     /inference="COORDINATES: protein motif:HMM:NF022101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HET7481152.1"
                     /translation="MRTSFIIAVASLGLILAAAGCGNAPVTNERAAQETTGETTGSSG
                     AASASLRGVVDSYKLAHEEIEAEGGEKDAGGYEIGYIVEPAEGWWEGDPGDLTWRGPR
                     GGETNHIEILPFDAETGLLVPQASIKLDVYDESGKEIQSKQLEFYYSEFYHYANNFSL
                     PKSGKYTLEAEISPPDFRRHGEENGEGKVFTEPVTVKFENVEISTKEE"
     gene            1689..2438
                     /locus_tag="VFJ72_16730"
     CDS             1689..2438
                     /locus_tag="VFJ72_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET7481153.1"
                     /translation="MNVFGLDIGGSGIKGATVDTESGELVSERVRIKTPQPATGQAIL
                     ETCIAVVRQSEWEGSVGCGFPAVIKDGVIQTAANVDDSVIGFDLRRELEKDLGNPVRV
                     VNDADAAGLAEVRWGAGRDVEGVVLMLTLGTGIGSALFVGGRLVPNTELGHLEIDGHD
                     AESRAGDGARKREDLSWEQYAKRLQRYLTTVENLFWPDLIVIGGGISKKSEKYFPYLE
                     TRTELVTATLLNEAGIIGAALAGAIETSVNR"
     gene            complement(2451..3020)
                     /gene="folE"
                     /locus_tag="VFJ72_16735"
     CDS             complement(2451..3020)
                     /gene="folE"
                     /locus_tag="VFJ72_16735"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET7481154.1"
                     /translation="MSGKVDLPRIERAVREILAAIGEDPDRDVMRETPERVAEAYAHL
                     FGGLHEDPDRYLDVAFAGDFGDPVMLRDVPVASLCEHHLLPFIGRAHVGYVPDGRVVG
                     LSEVVKVVEGYARRPQIQERLTAQVADALYGSLGSRGSIVVIEAEHTCMTMRGAEKPG
                     TITVTRAARGVYAEDAALRAEFLAHVSGR"
     gene            complement(3017..3712)
                     /locus_tag="VFJ72_16740"
     CDS             complement(3017..3712)
                     /locus_tag="VFJ72_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7481155.1"
                     /translation="MRYDTVFLDVDGTLLWVELDVEGYVRDLAPYASNGGLTVERATG
                     PMRESMRRHIEENIKHRTEESLFSFKRRNAEITAEALGVEAPAEVLTEVADRRISFNP
                     YPESERVMEDLRTMGAKLYVVSNWDILLEGILEDLGWTRYFDGVVVSAVIGSEKPDGA
                     IFDEALRLSGNTESADRVVHVGNDPMTDVFGASERGIDTVFVDRKGNGGAPGATYVLP
                     DLTALPDLVRGQL"
     gene            complement(3813..4043)
                     /locus_tag="VFJ72_16745"
     CDS             complement(3813..4043)
                     /locus_tag="VFJ72_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481156.1"
                     /translation="MDGTTLRVANIGSEADLELVRDALDTIGADYEHVDSDPEDLYPQ
                     TAYFQISTSLASDVEALFARLSDEHGFDAEVL"
     gene            complement(4054..5382)
                     /locus_tag="VFJ72_16750"
     CDS             complement(4054..5382)
                     /locus_tag="VFJ72_16750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET7481157.1"
                     /translation="MGRTASARRYDREILRLALPALGALAAEPTYVLVDTAIVGHLGT
                     SQLAALAIAAILLSTGVTLFNFLTYGTTAQVARMHGAGETAAAGRLGAQALWLSVGLG
                     GGLFALTLLLAAPAVALMGGEGEAGTMAVTYLRISALGLPFAMVALAGQGYLRGVGDL
                     RRPLVIVVAANVANLVLELLFVYGFGWGIAGSAWGTVLAQAGMGAAFVMELLRAPADS
                     RRPRWSLMRPMLRIGGDIAVRTGALLLSFALASAVVARMGEASLGAHQVAFQLFVFLA
                     LVLDAIAIAGQILVGRSLGAGDAAGAYDAARRMISWSVAVGVVFAAALLAGTGLIPRA
                     FTGDPAVIERAREIWPLFALMQPVGSAVFALDGILIGASDTRFLKWSMVAAFATFAPL
                     ALLSLAFGLGVTGVWVALNVLMAVRLLTCGGRFLGRRWAVVGASRDAGKV"
     gene            complement(5383..6483)
                     /locus_tag="VFJ72_16755"
     CDS             complement(5383..6483)
                     /locus_tag="VFJ72_16755"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET7481158.1"
                     /translation="MRDERLTKLARVLVDYSVAAGEGDQVLLAGGVAAEPLIKAVYAR
                     LLDVGATPIPQVALPGMQEVFFEHARDLHYEKTPPVARGIYEGADAFISIMAPTNTRA
                     LANVDPSKQQALGKRDKDLQEIVLGKDRWALTLFPTEALAQESEMGLAEYEEFVFEAM
                     ALNEEDPVRYWSEKAREQGRLIERLEQGREVRITGPETDLTLSIAGRKFLNGDGKKNM
                     PCGEVFTGPVEDSANGRVYFGVPVAVGGREVTGVRLRFEDGKVVEASAEKGEEYLNTM
                     LDADAGSRYLGELGIGTNYGIPRATKNILFDEKLGGTVHLAVGRSYAETGGKNDSSVH
                     WDLICDLRDGGALVVDGEEIQANGAFRDYEFG"
     gene            complement(6555..7334)
                     /locus_tag="VFJ72_16760"
     CDS             complement(6555..7334)
                     /locus_tag="VFJ72_16760"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET7481159.1"
                     /translation="MSVPVIFSTGSLYPFGLSRVFGWASAAGFDGVEVMMDERWDTHQ
                     AEYIEELAEGSGIPVLALHPPLYRGAWNLEPGETLVRVARLAQRLSVPVIVAHPPPPG
                     RPLRRWVAGPIQKAREQGVAVCVENMPRNAKRRMFSVRRGNCHRPEDLVGLGDVTLDT
                     SHLGASGEDPLRAWSVLGHQVRHIHLSDSRLSGKDEHRLPGHGKLPLRELLSAVGKSE
                     YPGAVSLELKPWPLGAPDPETIQERMREALGFVREGLGGEG"
     gene            complement(7331..8128)
                     /locus_tag="VFJ72_16765"
     CDS             complement(7331..8128)
                     /locus_tag="VFJ72_16765"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HET7481160.1"
                     /translation="MQERFSLENPAEIEPPEKGLYLGTSGWSYADWEGTVYPEATPSG
                     ARLAEYVKRFATVEIDSTFYGTPRRSAVQKWREVAPEGFLFAAKFPQEITHEKNLVAC
                     ERETETFVRTMSDLGDRLGPLLVQLPPSFTVEGMGVLEDFLKGLPEGYRYAVEVRHRS
                     WLRSDLAAMLREYGAALTLIDYPRMPRLEEATAGFSYIRWLGDRREFPSGHTHLKKER
                     DEDLRWWSGVVYRFLAEGRTVFAYANNHYQNHSPSTLERFLEIRRGG"
     gene            8259..9185
                     /locus_tag="VFJ72_16770"
     CDS             8259..9185
                     /locus_tag="VFJ72_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HET7481161.1"
                     /translation="MKVFYSDQFVLPLPEGHKFPMVKYSMLRERVAESGICGQGELRV
                     PRAVTDEEILRAHEPAYLKSVVNGTLTRQEMRRIGFPWSERMVERSRRASGGTLGACM
                     AALEDGVAANLAGGTHHAFADRGEGYCVFNDSAIAARALLEAGLVERVVVIDTDVHQG
                     NGTAAILRSDPRVFTLSIHGDKNFPFHKEESDLDIPLPDGADDSEFLSALEGGLECAL
                     EGSGSRIAIYLAGADPFEDDRLGRLKVSKSGLAERDRLVLESCSERGVPVAVTMAGGY
                     ARNVEDTVGIHFQSIRRAADVRTAIEKGASRA"
     gene            9252..10259
                     /locus_tag="VFJ72_16775"
     CDS             9252..10259
                     /locus_tag="VFJ72_16775"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7481162.1"
                     /translation="MTRGSSGGSCIIVGAGMSGLLAARELRAAGWDVTVLDKGRGVGG
                     RMATRRVGDGTCDHGAQFFTVRSERFERLVRDWLDTGVAEEWSRGFPDSSGGPREDGY
                     PRYRGSEGMTFIPKHLARGLDVRTGERVLRTRIQNGSWEAVCESGLRLSAKALLLTAP
                     APQSLEILDAGEVELSEGTRQQLEDIAYDPCLALLATVDREGLVPDPGGIQIKGEPLD
                     WISDNARKGISSIPALTIHAGPQWSRSHFSEDELYITNALLGFAGEQLGVELSPRVRE
                     TSLARWRYSWVTRPHPEPCLLAHEEPRLVFAGDAFGQPKVEGASLSGLAAADALLDRS
                     S"
     gene            10267..10701
                     /locus_tag="VFJ72_16780"
     CDS             10267..10701
                     /locus_tag="VFJ72_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481163.1"
                     /translation="MQLLFLTHLASTLVMTGIIWFVQVVHYPLFSRVGAAGFPAYSEA
                     HSHLTTFVVGPPMIVEAATAVLLVFVRPEDVPDYLVWIGLILLAAVWLSTAALQVPRH
                     TKLGLGFDPAVWTGLVATNWVRTAAWSLRAGVVLTMAGYAMK"
     gene            10802..11149
                     /locus_tag="VFJ72_16785"
     CDS             10802..11149
                     /locus_tag="VFJ72_16785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481164.1"
                     /translation="MGTRRVALKPHVGKIRRWVDEGRGDEWIAQELNTTSSSVQSFRS
                     RNSIYRRDPVRRGKVSEHPVVMEQSEEGLLLRTDVEDSDVFRREWRTHLGGSPTDLQL
                     VITQDRIYLEKIH"
     gene            11230..11442
                     /locus_tag="VFJ72_16790"
     CDS             11230..11442
                     /locus_tag="VFJ72_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481165.1"
                     /translation="MAGVLTRPELERVYRLLGWRILASKEGSGLTAHLSDLARDAATL
                     EEYESARDEYLGPILGGLERGENRDP"
     gene            11662..12672
                     /locus_tag="VFJ72_16795"
     CDS             11662..12672
                     /locus_tag="VFJ72_16795"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7481166.1"
                     /translation="MVAILTLFATLVVGGLAVLAQWARKSRGAEISLIVTLLALSLLV
                     AGLGALTGIGLLVAASEGRGAVSGSDQLAFVGAGVAAILAGLIGAGLCVPPLLKVTGR
                     PPKRAFWADPPMFLALWLLVVVLTNNAVSFLIFTQESDVSTLFPGGRLSLGEIVSSQL
                     PFVIVAVLGVGIGVRRTFRETLRRLGYTRISPSQAGIAVLFIILATLLSVGADAAFQT
                     LQPGLYKTVGDVTGSLFDTGGLSPASAILFSLLIGVGAALGEETLFRGAVQPRLGIPL
                     TSVLFASMHIQYGPSVLLVFVFVLSVGLGLLRRRVNTTASFIAHAGYNATGVLLSYFL
                     GF"
     gene            complement(12701..13000)
                     /locus_tag="VFJ72_16800"
     CDS             complement(12701..13000)
                     /locus_tag="VFJ72_16800"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7481167.1"
                     /translation="MTTIEIPAVKQRSDLLSVIGETLRAERNERGLTLREVAEGAHVS
                     VSYLAEIERGEKDPSSRVLESISSGLGMEISDLLMRVASAMEPAEVVPVSRSLAA"
     gene            complement(13010..13936)
                     /locus_tag="VFJ72_16805"
     CDS             complement(13010..13936)
                     /locus_tag="VFJ72_16805"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HET7481168.1"
                     /translation="MIESKNESNKQSKTQFQNGRTNRIGRHLGTSGGLKKTLATAESL
                     GCETVQIFVSNPQGWAEPVPRPDAEAFAEGSRRLGLDPVVVHSKYLINLASPKGESRE
                     RSARALAYEMVAAGALGASLVVVHSGSHGGDGEEAGLSRLVDSLDRAREIAAGLAPDS
                     GQVAEPIVENSVGGGTQLCSTFETLGEVSRRAGVRVCVDTAHAFVAGYDLSTPEGARG
                     VAAELREHLGSGVALLHLNDAKNEMGSRRDGHRKILNGQVARDAWAELFAGLPGVPVV
                     METPYADLEADAKEVLLVKELAGGLPVTPAGV"
     gene            14022..14468
                     /locus_tag="VFJ72_16810"
     CDS             14022..14468
                     /locus_tag="VFJ72_16810"
                     /inference="COORDINATES: protein motif:HMM:NF027852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HET7481169.1"
                     /translation="MAVEGKVAKILGNREIVINRGRNDGVRPGMTFEVFAPEGEEVWD
                     PDTGETLGTVEDVKAKAEVTEVKERLAVARLLGAESPFGAAGIGEMQENLQRIFGQMF
                     GEGVQVQGFGTTGAGDDPDLESMLGGPLDDLSKVQVGDAAREITRR"
     gene            14488..15186
                     /locus_tag="VFJ72_16815"
     CDS             14488..15186
                     /locus_tag="VFJ72_16815"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7481170.1"
                     /translation="MSNLSRLPVTFALISACVVVYVGVAYAGSVAEIAPLNVGLVNQP
                     SPMLQRGALVPDLVARGEYYRLFTSVFLHSGAIHIGFNMLSLYYLGSFVEESFGRGRF
                     LALYILSGLAGGIAYLYFGGFNTPAVGASGAIFGLLGGVLGYSLRRGTFSWQNPIVRQ
                     LLILTALNLYLGFSIPNISNTAHIGGLVGGAVFGWLTAPTVYARKRLPTLAPVLIVLG
                     AEVLLFGVWLAYFV"
     gene            complement(15202..15936)
                     /locus_tag="VFJ72_16820"
     CDS             complement(15202..15936)
                     /locus_tag="VFJ72_16820"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HET7481171.1"
                     /translation="MDPVTAFLLALIAAAGFMVSSSLGLLGRKTMFAGRSYPAAVAAG
                     ILLALAFADLFPEGLELARDAAILGFVGGFALLFVIELFTHAHTHHVPEEGVPRHMLG
                     SFVLGLMIHNLADGFVLGVGARTVEVTSGLVGLGIVIHQLPVGISLAAVFVASGASRS
                     RVLRTTALLGLAIPLAAGLTVLLPVPGSGTLGLLTGMAGGVLTYVGAAHLLPEARSEH
                     PNKSSGVALVASLLVTTVLILTVLHG"
     gene            complement(15974..16366)
                     /locus_tag="VFJ72_16825"
     CDS             complement(15974..16366)
                     /locus_tag="VFJ72_16825"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET7481172.1"
                     /translation="MDVGAVVGKLRGGGFKVTPQREAIVRAVAAEQHQSMEGIRARCP
                     GVGMVTVYRTLDLLSELGLVRRLDLGDRPRYELAEDHHHHLICEGCGEITEFDLCPLD
                     PRLLDPGAVGFEVRSHSVEVYGRCATCR"
     gene            complement(16478..17308)
                     /gene="lgt"
                     /locus_tag="VFJ72_16830"
     CDS             complement(16478..17308)
                     /gene="lgt"
                     /locus_tag="VFJ72_16830"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET7481173.1"
                     /translation="MTFALLSLPSPPSPIIFQIGPFPLRYYGLFIALGIVAATWLAGR
                     ELERKGYDRALALDSLFFLVPLGFVGARLYHVITDYELYRDDPFPAVFMVQNGGLGIY
                     GAVVGGLLGLLIFARIRGISVLAFADAAAPGVILAQAIGRWGNYFNQELFGRPSNLPW
                     AIRIAPENRPVGYSDATSFHPTFLYECIWNVIVCLILLWVARRFSERLKDGDIFFLYV
                     SLYSVGRFFVETLRIDPAFLIGGTIRGNLLVSSALALGFAMILLLRHSGSGRKRPTSP
                     "
     gene            complement(17325..19385)
                     /gene="mrdA"
                     /locus_tag="VFJ72_16835"
     CDS             complement(17325..19385)
                     /gene="mrdA"
                     /locus_tag="VFJ72_16835"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET7481174.1"
                     /translation="MLRVPKRRHHEPVPAKKRGVTPPATKGNMQVRVVTIAVLVAVLF
                     AILGTRLWYLQVLTSENFTKSAQQTHTREVKIPAQRGVIKDRNGDVLANNIPGLNVTV
                     IPNEISREKVQKLAKIMQADVKAVTDRYNAAMDPETGNRYGSMLVKENADRDAVTYVM
                     ERTNEFRGVTVTDDFVRNYPQGELAAHVLGYTGAVTAEELKQKHFKGLSNDSVVGKSG
                     VELTYENELRGEPGSKKYNVDAVGRVVTVRRADGTRADGAPEVSPELGRPASITDPQP
                     GKDLTLTLDMKLQKTAESELDAAIVRAQQEGYSGEGGAVIAMDPRNGEILAMASRPTF
                     DPQLFVGGISGADEAETFKYLNSEYANSPFTNRAIAGAYPGASTFKVFTGLAGFAYGV
                     LDAATTVTDNGGCFEPAGTTGSCWYSWRQSYGTGSTHGTQNYAEAIADSNDKFFYQVA
                     DWLWQKTNDENLLPDFYKRFGFGSTTGVDLPGETPGRIPDKQWFESYQKEVYGYLDRP
                     WGIADWINLSIGQGDILVSPIQMAQAYSAVENDGTLVTPHVAKAITDRDGKKQEISPK
                     PEGKIEFNESARNELLKGLRGVTGPEGTAAPAFEDSKLSIVGKSGTGERGNKDPVNWF
                     AGWAENEKDPLVVVVMVEGGGHSEVTAAPAVRKILEARYGVEDAAAQQDSGLVSTYAG
                     TPAQ"
     gene            complement(19389..20069)
                     /locus_tag="VFJ72_16840"
     CDS             complement(19389..20069)
                     /locus_tag="VFJ72_16840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HET7481175.1"
                     /translation="MRFANIALNLMSLVLVVAGLALITTFFFGSPFVSSGNAQESSSV
                     SKDEFNVPVLDESAQKEPAIAPAAPAEKDKEKKPEPAIPVPKDKTLRITIPKMARVDD
                     TDIPYTTGDDEQALHDHTAIHLKGTGFPWEKEANVYIAGHRLGYLNTKSFLAFYDLNV
                     LTEGDKIFITDSMGRRYVYKVFKDFVVDPSDVYITRPVKNKNIVTLQTCTLPDYSQRL
                     IVQAERVA"
     gene            complement(20185..20733)
                     /locus_tag="VFJ72_16845"
     CDS             complement(20185..20733)
                     /locus_tag="VFJ72_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481176.1"
                     /translation="MTALPTTKKRDPAPRRASGYLLGGLAIVVLVALVWALLAGRGFE
                     PGEKMEAEGIKDVAVSEEDTLYPPSDVLRFSEAPAEIYVYVAVDGLPSGTKLTARVDR
                     AGRESVLGRLFSSAADIEVVDGQEDQLSPASRGVAGVVKFAVRTRSGESLPAGNYTVI
                     IRRAGANVQDSEVARKLFVVRG"
     gene            complement(20730..21272)
                     /locus_tag="VFJ72_16850"
     CDS             complement(20730..21272)
                     /locus_tag="VFJ72_16850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7481177.1"
                     /translation="MGPRFVKQAALFYGFLLVAAALWNGLRDRALFAVGDSVPESVSL
                     GVLAAAVTVSAGLLTYRAVPLLRRLADELAPMLVDGTSRRDLVLLSVFSGIGEEVFFR
                     GAVQPEFGIVIASLAFGLLHVGPDRRFVIWTVWAVAAGFLFGALFIATGGIAAPVIAH
                     TLHNGATFLVWKRARSEKRP"
BASE COUNT         3721 a         7203 c         6837 g         3698 t
ORIGIN      
        1 ccgaccgcga cttgacgaac taccgccccg tacattaccc tgaccgtgaa gttattgcaa
       61 atgattgtca ttagtgtcaa gcgccgaagt ggcgggaggt gtaacgaggg tgttgggatc
      121 tttcctggtg atgttgcgag aggggttcga ggcttctctg ccggtcgcca tagtgctggc
      181 gtatctgacg cagaccggac gcaaggagga cgcccgcggg gtttggtacg gggtcggcgc
      241 ggcggtcctg atctctatcg ttaccgcagg ggtcttgttc gtgaccgcga acggcctcga
      301 aggaacggcc gggatctact tcaagagtgg agccatgtgg cttgcagtag ccttcctcac
      361 ttacatggtc ctctggatgc gccggcaggc tcgcacgatg gcccagggga tacggcgtaa
      421 cgtggatagc gcggtcgagc gcggcggcct tctggcgctg gggatgctcg tcttcgtcat
      481 ggtcttgcgc gagggtatag agaccgggtt gttcatgttc ggcattaccc agaacgcgac
      541 gcctctcggc gtcgggatag gcgcggtagc tggtacggcc gcggctgtcg gcctgggata
      601 cgcggtctac gtcggcggca agcgcataaa catgggcgcg ttcttcaaga tcacgggcat
      661 cctgctcatc gtggtggcgg ccggtctgct ggcgcgcggg gtggcgatca tggagattgg
      721 gggcatcatc cccgctttct tctatccggt ctgggatctc tcctctatac atttgctctc
      781 tagcggcagc gtgagcggcc agttcctgac ggcgttcctc ggctgggacg cgaagccgga
      841 ccttctggag gtcggggtgt gggcggtcta cctgctgacg gtcggcttct tcttcctgcg
      901 cccgcagacc cggacggcgg cccaaccggc agcggcccac aactagtccc aacgctccgc
      961 ggagcatata gaacaggaga caacttgaga acctctttca ttatcgccgt ggcatcgctc
     1021 gggctgatac tagccgcggc cggttgcggc aatgctcccg tcacgaacga gcgggccgcg
     1081 caggagacca cgggagagac taccggatct tccggggcgg cctcggcgtc gctgcggggg
     1141 gttgtggact cctacaagct cgcgcacgag gagatagagg ccgagggcgg cgagaaggac
     1201 gcgggcgggt acgagatcgg atacatcgtc gagccggcgg agggctggtg ggaaggagac
     1261 cccggcgacc tcacgtggcg cggaccgcgc ggcggagaga cgaaccacat cgagatcctg
     1321 ccgttcgacg ccgagacggg actactggtc cctcaagcca gcatcaagct cgacgtctac
     1381 gacgagagcg ggaaagagat ccagagcaag cagctagagt tctactactc ggagttctac
     1441 cactacgcca acaacttcag ccttccgaag agcggaaagt acacgctgga ggcggagatc
     1501 tcgccgcctg acttccgccg ccacggtgag gagaacggcg agggcaaggt cttcaccgag
     1561 cctgtaacgg tgaagttcga gaacgtcgag ataagcacca aggaagagta gctcctcccg
     1621 gagagtcttg gcggcgggcc ggtcgctccc tgtagaatag acgctggact gctctacggg
     1681 gaggtagcat gaacgttttc ggcttagata tcggcggctc cgggataaag ggcgcgacag
     1741 ttgatacgga aagcggagag ctggtctccg aaagggtcag gatcaagact ccccagcccg
     1801 cgaccggtca ggcgatactt gagacgtgca tcgcggtggt ccggcaatcg gagtgggaag
     1861 gctccgtggg gtgcggcttc ccggcggtaa ttaaggacgg cgtcatacag accgcggcca
     1921 acgtggacga ctcggttatc ggcttcgatc tcaggagaga gctggagaag gatctgggga
     1981 acccggtgcg ggtcgtcaac gacgccgacg cggcgggact cgcggaggtg cgctggggag
     2041 cgggacgcga cgtggagggc gtggtcctga tgctgacgct cgggaccggc atcgggagcg
     2101 cgttgttcgt cggcgggagg ctcgtcccga acacggagct cggccacctg gagatagacg
     2161 gccacgacgc cgagagccgc gcgggcgatg gcgcccgcaa gcgcgaggac ctgagttggg
     2221 agcagtacgc caagcgcctt cagaggtacc tgacgaccgt cgaaaacctg ttctggccgg
     2281 acctgatcgt gatcggcggt ggcatctcca agaagtccga gaagtacttc ccgtacctgg
     2341 aaacccggac cgaactcgtc acagccacac tacttaacga ggccggcatc atcggcgccg
     2401 cgctcgccgg agctatcgaa acgtctgtga accggtagaa gccccggtct ttaccggccc
     2461 gaaacgtgcg ccaggaactc cgcccgtaga gccgcgtcct cggcgtacac gccgcgagcc
     2521 gcccgcgtga ccgtgatagt gccgggcttc tcggcgccgc gcatcgtcat acacgtgtgc
     2581 tccgcctcga tcacgacgat ggagccccgc gatccgagag atccgtacag ggcatcggcg
     2641 acctgggcgg tgaggcgctc ctggatctgg ggccgcctcg cgtaaccttc caccaccttc
     2701 actacctccg acagcccgac cacccgcccg tccggcacat aaccgacgtg cgccctgccg
     2761 atgaacggca ggaggtgatg ctcgcacagg ctggcgaccg gcacgtcccg cagcatcacc
     2821 gggtcgccaa agtctccggc aaaggccacg tccaggtagc ggtccgggtc ctcgtgcagg
     2881 ccgccgaaga ggtgagcgta agcctccgcg acccgttccg gcgtctcgcg catcacgtcg
     2941 cggtcagggt cctcgccgat ggccgccagg atctcgcgca ccgcccgctc gatgcgcggc
     3001 aggtctacct tcccgctcac agctgacctc gcaccaggtc cggcaaggcg gtcaggtccg
     3061 ggagcacgta ggtcgccccc ggtgccccgc cattgccctt ccggtccacg aagaccgtgt
     3121 cgatcccgcg ctccgacgct ccgaacacgt ccgtcatggg gtcgttgccg acgtgaacga
     3181 cgcggtccgc gctctccgtg tttccgctca gacgcagggc ctcgtcgaag atggccccgt
     3241 ccggtttctc cgagccgatg accgccgaga ccacgacgcc gtcgaagtag cgcgtccagc
     3301 cgaggtcttc gaggatgcct tccaggagga tgtcccagtt cgagacgacg tagagcttcg
     3361 cgcccatcgt ccgcaagtct tccatcaccc gctccgactc cgggtacggg ttgaaggaga
     3421 tcctgcggtc cgcgacctcc gtcagcacct cggcgggagc ctcgacgccg agagcctccg
     3481 ccgtgatctc ggcgttgcgg cgcttgaaac tgaagaggct ctcctcggtg cggtgcttga
     3541 tgttctcctc tatgtgacgc cgcatgctct cccgcatggg accggtcgcc cgctcgaccg
     3601 tcaggccgcc gttggatgcg tagggcgcga ggtcccggac gtagccttcc acgtccaatt
     3661 cgacccacag gagcgtccca tcaacgtcca ggaacacggt atcgtagcgc attctcgcct
     3721 ctctacttgc tgcatatcct cttgaccgga gacaaccgcg ggctcgctcc ggccacagga
     3781 tcagagtata aaccgcaggt tgcggagggc tcctagagga cctccgcgtc gaagccgtgc
     3841 tcgtcggaaa gacgtgcgaa gagcgcttct acgtcgctgg cgaggctggt ggagatctgg
     3901 aagtacgccg tctgcgggta caggtcctcc gggtcggagt ccacgtgctc gtagtccgct
     3961 ccgatggtgt ccagcgcgtc ccgcaccagc tccaggtccg cctcgctgcc tatgttagcc
     4021 acgcgcaggg tagtgccgtc catgcgtaga agtctacact tttccagcat cccgcgaggc
     4081 cccgactacg gcccagcgcc gcccgaggaa gcggccgccg caggtcaaaa gacgcaccgc
     4141 catcaacacg ttcaacgcga cccagacgcc ggtaacgccg agcccgaagg cgagtgacag
     4201 tagcgccagc ggagcgaagg tcgcgaacgc ggcgaccatg gaccacttga gaaaacgagt
     4261 gtcgctcgcc ccgatcagga ttccgtccag cgcgaagacc gcactcccta cgggctgcat
     4321 cagggcaaag agcggccaga tctcccgcgc ccgctcgatc accgccgggt ctccggtgaa
     4381 ggcgcgcggg atcagccccg tgccggcgag gagcgccgcg gcgaagacta ccccgacggc
     4441 gacggaccag gagatcatgc gccgcgcggc gtcgtaagcg ccggccgcgt ccccggcccc
     4501 gagggagcgg ccgaccagga tctgcccggc tatcgcgata gcatccagca cgagcgccag
     4561 gaacacgaag agttgaaacg ccacctgatg cgcccctaga gacgcctccc ccattcgcgc
     4621 cacgaccgcg ctcgccagcg cgaacgagag caggagcgcc cccgtccgca ccgcgatgtc
     4681 gcccccgatc ctcaacatag gccgcatcaa ggaccaccgc ggacggcgcg aatcggcggg
     4741 cgcgcgcaac aactccatca cgaacgccgc gcccatgccg gcctgggcca gcaccgtacc
     4801 ccacgccgat cccgcgatgc cccacccgaa cccgtacacg aacagcaact ccagcaccaa
     4861 gttggcgacg ttcgcggcga ccacgatcac gagcggccgc cgcaggtctc ccacgccccg
     4921 caggtaaccc tgcccggcca gcgcaaccat tgcgaacggc aacccgagcg cggagatcct
     4981 gaggtacgta acggccatcg tcccggcctc cccttccccg cccatgagcg ctacggcggg
     5041 cgcggcgagg agcagcgtca gggcgaacag cccgcctccg agcccgaccg agagccagag
     5101 cgcctgcgcg ccgagccgac cggcggcggc cgtctcaccc gcgccgtgca tgcgggcgac
     5161 ctgggccgtg gtgccgtagg tcaggaagtt gaagagcgtg acgccggtgg agaggaggat
     5221 cgcggcgatg gcgagggcgg cgagctggga cgtgccgagg tgtccgacga tggccgtgtc
     5281 cacgagtacg tacgtcggtt cggcggcgag ggcgccgagc gcggggaggg ctagacgcag
     5341 gatctcacgg tcgtagcgcc gggctgacgc ggtccggccc acttatccga attcgtagtc
     5401 ccggaaggcg ccgttcgcct ggatctcctc cccgtccacc acgagcgctc cgccgtcgcg
     5461 caggtcgcag atgagatccc agtgaacgct ggagtcgttc ttgccgcccg tctccgcgta
     5521 ggagcgtccg acggcgaggt ggaccgtgcc gccgagcttt tcgtcgaaga ggatgttctt
     5581 cgtcgcgcgg gggatgccgt agttcgtccc gatccccagt tctcccaggt accgtgaccc
     5641 cgcgtcggcg tcgagcatgg tgttcaggta ctcttcgccc ttctccgcgc tcgcctcgac
     5701 gaccttcccg tcctcgaagc gcaggcgcac tcccgtaacc tcgcgccccc cgaccgccac
     5761 cggcacgccg aagtagacgc gcccgttcgc cgagtcctcg accgggccgg tgaagacctc
     5821 gccgcacggc atgttcttct taccgtcgcc gttcaggaac ttgcgcccgg ctatcgagag
     5881 cgtcaggtcc gtctccgggc cggttatgcg gacctcgcgg ccctgctcca gccgttcgat
     5941 caatcgcccc tgctcccgcg ccttctcgct ccagtaccgg accgggtctt cctcatttag
     6001 cgccatcgcc tcgaagacga actcctcgta ctccgcgagc cccatctcgg actcctgggc
     6061 gagcgcctcg gtcgggaaaa gcgtcagcgc ccagcggtcc ttgccgagca ctatctcctg
     6121 caggtccttg tcccgcttgc cgagcgcctg ctgcttggac gggtcaacgt tcgccagggc
     6181 acgcgtgttc gtcggggcca tgatggagat aaaggcgtcg gcgccctcgt agatcccgcg
     6241 agccaccggc ggcgtcttct cgtagtggag atcccgcgcg tgctcgaaga acacctcctg
     6301 catccccggc agcgcgacct gcggtatcgg ggtggcgccg acgtcgagga gccgggcgta
     6361 gaccgctttg atcaggggct ccgccgcaac ccctccggcg agcaagacct gatccccctc
     6421 ccccgccgcg accgagtaat ccaccaacac ccgcgccagc ttcgtcagcc gttcgtctct
     6481 cattcaacct cccgcaaagt tagaaatccg cccgtgagct taccacccgc gcgcggacga
     6541 tcgtcggatc gtcgtcaccc ttcaccaccg agcccctccc tgacgaaccc cagcgcctcc
     6601 cgcatccgct cctggatcgt ctccgggtcc ggcgccccga gcggccaggg cttcaactcc
     6661 aaactgacgg cacccggata ctcgctcttg cccacggccg atagaagctc ccgaagcggc
     6721 agctttccgt gtccggggag ccgatgctcg tccttgccgc tgagcctcga atcggagagg
     6781 tggatgtgcc gcacctgatg tcccagtacc gaccacgccc gcaacgggtc ctcgccgctc
     6841 gcccccaggt ggctggtatc cagcgtaacg tcgcccaacc ccaccaggtc ctccggccgg
     6901 tggcagttcc cgcgccgcac gctgaacatc cgccgcttcg cattgcgcgg catattctcg
     6961 acgcagacgg cgacgccctg ctctctcgct ttctggatcg gccccgcgac ccagcgccgc
     7021 agcggcctcc ccggtggcgg cggatgggcc acgatgaccg gcacagagag ccgctgggcg
     7081 agccgggcga cgcgcaccag cgtctccccc ggctccaggt tccacgcccc ccgatacagc
     7141 ggcggatgga gcgccagcac cgggatgccg ctgccctccg caagctcctc gatgtactcc
     7201 gcctggtgcg tgtcccagcg ctcgtccatc atgacctcca cgccgtcgaa gcccgcagcg
     7261 gacgcccatc cgaagacccg gctcaagccg aacgggtaca gggagccggt gctgaagatg
     7321 actgggacgc tcacccgccc cgccggatct ctaaaaatcg ctccagcgtg gagggcgagt
     7381 ggttctggta gtggttgttc gcgtaggcga agacggttcg tccctcggcg aggaacctgt
     7441 acactacccc agaccaccag cgcagatcct cgtcgcgctc cttcttcaga tgcgtgtgcc
     7501 cggacgggaa ctcgcgccgg tcccctagcc agcggatgta cgagaacccc gccgtcgcct
     7561 cctccagccg gggcatcctc ggataatcta tgagcgtcaa cgccgcgccg tactcgcgca
     7621 gcatggcggc caggtccgag cgcagccagc tccggtgccg cacctccacc gcgtacctgt
     7681 agccttccgg caaccccttc aagaagtcct ccagcacgcc catcccttcc acggtaaacg
     7741 acggcgggag ttgcacaaga agcggcccga gacggtcacc gagatcgctc atggtgcgga
     7801 cgaaggtctc agtctcccgc tcgcacgcga cgaggttctt ttcgtgcgtg atctcctgcg
     7861 ggaacttcgc cgcgaacagg aacccctccg gcgccacctc ccgccacttc tggaccgccg
     7921 agcggcgcgg cgtcccgtag aaagtcgagt ctatctccac cgtcgcaaat cgcttcacgt
     7981 actccgcgag ccgggcgccg gacggggtgg cctcggggta gaccgtcccc tcccagtccg
     8041 cgtacgacca gccggacgtg ccgaggtaga gccccttctc gggcggttcg atctccgccg
     8101 ggttctccag agagaagcgt tcctgcatag atagcctcca cggactccgc aaaaattcga
     8161 tgctaccaca gccgcccgcg ccggtttatg aacgcagtcg ccgcgcgagg gcgcgagatg
     8221 tattagcctc tacgcatgag atccaaggcc agggaccgat gaaagtcttc tacagcgacc
     8281 agttcgtcct gccactcccg gaggggcaca agttcccgat ggtcaagtac tcgatgctgc
     8341 gcgagcgcgt ggcggagtcc ggcatctgcg gccagggcga gttgcgtgtc ccgcgagctg
     8401 tcacggacga ggagatactt cgcgcccacg agcccgcgta cctgaagagc gtcgtcaacg
     8461 gcaccctgac gaggcaggag atgcggcgga tcggcttccc ctggtccgag aggatggtcg
     8521 agcgctcccg gcgcgcctcg ggcggcacgc tcggcgcctg catggccgcg ctggaggacg
     8581 gcgtggccgc gaacctggcc ggaggaaccc accacgcctt cgcggaccgg ggcgagggct
     8641 actgcgtctt caacgactcc gccatcgcgg cgcgggctct gctggaggcc ggtctcgtcg
     8701 agcgggtcgt cgtgatagac accgacgtgc atcaggggaa cgggacggcg gcgatcctgc
     8761 gctccgaccc ccgagtgttc acgctctcca tccacggcga caagaacttc ccgttccaca
     8821 aggaggagag cgacctggac atcccgctcc ccgacggcgc ggacgactcc gagttcctct
     8881 ccgccctcga agggggccta gagtgtgctc ttgaaggctc gggctcgcgg atcgcgatct
     8941 acctggccgg agcggacccg ttcgaggacg accggctcgg acgcctgaag gtgagcaaga
     9001 gcggcctcgc ggaacgcgac cgcctcgtcc tggaatcgtg cagcgagcgc ggcgtcccgg
     9061 tcgccgtgac gatggccggc ggctatgccc gcaacgtcga ggacaccgtc ggcatccact
     9121 tccagagcat ccgccgcgcg gcagatgtgc gaaccgcgat agagaaggga gcatctcgtg
     9181 cataacggta gcgccgctcc cagccgcgcg cccagcggct tcgtcctccc gcaagccggg
     9241 gcggtagctc catgacgcgg ggctcatcag gcggctcgtg catcatcgtc ggggccggga
     9301 tgtcgggcct gctcgcagcc cgcgagttgc gagcagccgg gtgggacgtg acggttctcg
     9361 acaagggccg cggcgtcggc ggccggatgg cgacccggcg cgtgggcgac ggtacctgcg
     9421 accacggcgc ccagttcttc accgtcagga gcgagcgctt cgagcggctc gtcagggact
     9481 ggctcgacac gggagtagcg gaagagtggt cgcgcggctt ccccgactcc tccggcgggc
     9541 cgcgcgagga cggttatccg cgctaccgcg gctcggaggg catgaccttc atccccaaac
     9601 acctcgcccg cggcctggac gtgcggaccg gagagcgggt gctgcgtacc cgcatccaga
     9661 acggaagctg ggaggccgtc tgtgaatcag gtctgcgcct gagcgctaag gccctgctcc
     9721 tcaccgctcc ggctccgcaa tcgctggaga tattggatgc cggagaggtc gagctttcag
     9781 aaggcacacg ccagcagctc gaagacatcg cctatgaccc ctgcctggcc ctgctcgcta
     9841 ccgtggaccg ggagggtctc gtccccgatc ccggcggcat ccagataaaa ggcgaaccgc
     9901 tggattggat ctccgacaac gcccggaagg gcatctccag catacctgcc ctcaccatcc
     9961 acgcgggacc gcaatggagc cggtcccact tcagcgaaga cgagctatac ataacaaacg
    10021 cgctcctcgg gttcgcgggc gaacagctcg gcgttgagtt gtctccacgt gtccgggaga
    10081 cgtccctcgc ccgctggcgc tatagctggg taacgcggcc gcacccggag ccctgcctcc
    10141 tcgcgcatga ggagccccga ctcgtcttcg ccggtgacgc cttcgggcag ccgaaggtcg
    10201 aaggggcctc tctctccggc ctcgccgccg ccgatgcgct gctcgaccgc agcagttaaa
    10261 ggattcgtgc agctactctt cctcacacat ctcgcctcga ctctggttat gaccgggatc
    10321 atctggttcg tccaggtcgt ccattatccg ctcttctccc gcgtcggcgc cgcaggattc
    10381 cccgcctact ccgaggccca ttcccacctc acgacgtttg tagtcggccc gccgatgatc
    10441 gtggaggcgg cgacggccgt actcctggtg ttcgtccggc ccgaagacgt accggactac
    10501 ctggtctgga tcggcctgat cctgctggcg gccgtgtggc tctcgaccgc ggccctgcag
    10561 gtcccgcgtc acacgaagct cgggctcggg ttcgaccccg ccgtatggac gggcctcgtg
    10621 gcgaccaact gggtccgcac cgcggcctgg agcctgcgag ccggggtcgt cctgacgatg
    10681 gcaggctacg cgatgaaata ggagatccag agtcccggcg cagacgcaaa catatctggc
    10741 gcggagtaac aaaaagtggt acatataaac tataaatcta aagcggtgaa gagagggagc
    10801 cgtgggcacg agaagagtag cgttaaagcc tcatgtcggt aagatccggc gctgggtaga
    10861 tgagggccgg ggtgacgagt ggatcgccca ggagctcaat acgacctcgt cgagcgtgca
    10921 gagcttccgg tcgaggaact ctatctaccg gcgggacccc gttcggcgcg ggaaggtatc
    10981 cgagcacccg gtggttatgg agcagagcga agagggactg ttgttgagga cggacgtgga
    11041 ggattccgac gtcttccgca gagagtggcg cacccatctg ggcggctccc ccaccgatct
    11101 tcaactcgta atcacccagg accgcattta cctggagaag attcactgaa cccggaagag
    11161 agagcgccgg tagacctgag cctcacccag gaagaggccg acgccctgca cgaagcgctg
    11221 gaagagtctt tggcgggcgt cctcacccgc cccgagttgg agagggtcta ccgtctcctc
    11281 ggctggcgta tcctggctag taaggagggc tccggcctga ccgcgcacct ctccgacctg
    11341 gcgcgcgacg ccgcgacgct tgaagagtac gagagcgccc gcgacgaata cctcggcccc
    11401 atactcggcg ggctggagcg cggcgagaac cgcgacccgt aagacaaact aactgttatc
    11461 aaactgttac ctgcgcgtgg cggtaatgta atccgctggc gttatattac cggcaatggg
    11521 ctttccccct tccggcccag aagacgctcc cggaccacgg atttcgcgcg gtccgggatt
    11581 ttttctgaaa ttttttctgt gataacctct ttcgcggacc catcgggccg tctttatccc
    11641 gctgactaga aaggcttgcg gttggttgcg atcctgacct tgttcgccac cctcgttgtc
    11701 ggaggcctgg cggtgctcgc gcagtgggcg cggaagagcc gcggcgcgga gataagccta
    11761 atagtcacgc tgctggctct ctcgttgctc gtggcggggc tcggcgcgtt gacgggcatc
    11821 gggttgctgg tcgcggcgtc ggagggccgg ggcgccgtct ccgggtcgga tcaactcgcg
    11881 ttcgtcggcg cgggggtggc ggctatcctt gcgggtctca taggagcggg gctttgcgtg
    11941 ccgccgctgc tcaaggtcac gggccgaccg ccgaagcgcg ccttctgggc cgatcccccg
    12001 atgtttctgg cgctgtggct cctcgtggtc gtgctcacga acaacgcggt gagcttcctt
    12061 atcttcacgc aagaatccga cgtgagcacc ctgttccccg gcggcaggct ctctttaggg
    12121 gagatcgtct ccagccagct accgttcgtg atcgtggccg tactcggggt tggtatcggg
    12181 gtccggcgta ccttccgcga gacgctgcgc aggctcggtt acaccaggat ctcaccctcg
    12241 caggccggga tagccgtcct cttcattatc ctcgccaccc tcctgtcggt cggcgcagac
    12301 gcggctttcc agacgcttca gccgggcctg tacaagaccg tcggggatgt tacgggcagc
    12361 ctcttcgaca cgggcgggtt gagccccgcc tcggcaatac tattctcgct cctgatcggc
    12421 gtcggagccg cgctgggaga ggagacgctc ttccgcggag cggtccagcc gaggctaggc
    12481 atccccctta cgtccgtgct gttcgcctcg atgcacatcc agtatgggcc atccgtcctg
    12541 ctcgtctttg tcttcgtcct ctccgtcggc ctcggtctgc tccgccgccg cgtcaacacg
    12601 accgcaagct tcatcgccca cgcgggctac aacgcgacag gggtgctgct ctcgtacttt
    12661 ctggggttct agaagacgtt ctacagatta ggcgcgagcg tcaggcggcc agcgaccgtg
    12721 agaccggcac cacttccgcg ggctccatgg cgctcgccac ccgcatgagg aggtcgctga
    12781 tctccatgcc cagcccggag gagatgctct ccagcaccct ggaggatggg tccttctcgc
    12841 cgcgctctat ctcggccaga taagagacgg agacgtgggc gccttcggcg acttcccgga
    12901 gggtaaggcc gcgttcgttg cgctcggccc gcagggtctc cccgatgacc gagaggaggt
    12961 cgctccgctg cttaaccgcc ggtatctcga tagtggtcat ggacaaattc tataccccag
    13021 ccggtgtaac aggcaacccg cccgcaagtt ccttgaccag caggacctcc ttggcgtccg
    13081 cctccaggtc cgcgtagggc gtctccatca ccaccgggac gcccggcaga ccggcgaaca
    13141 gctccgccca cgcatccctg gctacctgcc cgttcaggat cttgcggtgc ccgtcccggc
    13201 ggctgcccat ctcgttcttc gcgtcgttca gatgcagcaa ggccaccccc gaaccgagat
    13261 gctcgcgcag ctccgccgcg acaccccgcg ctccctccgg cgtcgagagg tcgtagcccg
    13321 cgacgaatgc gtgggcggtg tccacgcaga cgcgaacccc ggcgcggcgg gatacctcgc
    13381 cgagagtctc gaacgtcgag cagagctgcg tgccgccacc gaccgagttc tccacgatag
    13441 gttccgcgac ctgcccggag tcaggagcga gtcctgcagc gatctcgcgg gcgcggtcca
    13501 gcgagtccac gaggcgcgag agccccgctt cctccccgtc gccgccgtgg ctgccggagt
    13561 ggacaacgac gagggacgcc ccgagcgcgc cggcagcgac catctcgtag gccagcgcgc
    13621 gggcggagcg ctcgcggctc tcccctttcg gcgaggcgag gtttatcagg tacttcgagt
    13681 gaacgactac cgggtcgagc cccagtctcc ggctcccctc ggcgaaggct tcggcgtcgg
    13741 gacggggtac aggttcggcc catccctgcg ggttcgagac gaagatctgg accgtctcgc
    13801 agcccaggct ctcggccgtc gcgagcgtct tcttcagccc accggatgtc ccgagatgcc
    13861 gaccgatccg gttcgtgcgc ccattctgga attgcgtctt cgattgctta ttcgattcgt
    13921 tcttcgattc gatcatggcg cagattgtag ccattaccgg ctgagagcgt tgtgagagat
    13981 ataatcttcg ggaagtaaac ggatgagaag gagcgtaccg catggcggtc gaaggcaagg
    14041 tagcgaagat attaggcaac cgggagatcg tcataaaccg gggccgtaac gatggagtgc
    14101 gtccgggcat gacgttcgag gtcttcgccc ccgagggcga ggaggtctgg gacccggaca
    14161 ccggagagac gctcggtacg gtcgaggacg tgaaggcgaa agccgaggtc acggaggtga
    14221 aggagcggct cgcggtcgcc cggctgctcg gcgctgagtc tccgttcggg gcggcgggca
    14281 tcggcgagat gcaggagaac ctccagcgga tatttggcca gatgttcggg gagggcgtac
    14341 aggtgcaggg cttcggcacc acgggcgccg gcgacgaccc ggacctggag tccatgctcg
    14401 gcggcccgct ggatgacctc tccaaggtgc aggtcggcga cgctgcgcgc gagataacgc
    14461 gccgctgaaa gcgctgctga agtccgtttg agcaacctct cgcgcctgcc ggtcaccttc
    14521 gccctgattt cggcgtgcgt cgtggtctac gtcggcgtgg catatgccgg tagcgtagcg
    14581 gagatagcgc cgctcaacgt gggcctcgtc aaccagccgt ctccaatgct ccagcgcggc
    14641 gcgctcgtcc cggatctggt agcgagaggc gagtactacc ggctcttcac cagcgtattc
    14701 ctgcactccg gggccatcca catcggcttc aacatgctgt cgctctacta cctgggctcc
    14761 ttcgtcgagg agtcgttcgg gcgggggcgt ttcctcgcgc tctacatcct gagcggtctg
    14821 gcgggcggca tcgcgtacct gtacttcggc ggcttcaaca ctccggcggt cggggcttcc
    14881 ggcgcgatct tcggcctgct cggcggcgtg ctggggtact cgctgcgccg ggggacgttc
    14941 tcctggcaga acccgattgt ccgccagcta ctaatcctta ccgccctgaa cctctacctc
    15001 ggcttttcca tccccaacat atccaacacc gcccacatag gtggcctggt aggaggcgcg
    15061 gtcttcgggt ggctcacggc ccccacggtc tacgcccgta agaggctccc cacgctggcg
    15121 ccggtcctga tcgtgctcgg cgcggaggtc ttgctgttcg gcgtctggct ggcctacttc
    15181 gtgtaaccct aatagtctcg cctacccgtg caagacggtc aggatcagga cggtcgttac
    15241 gagcagagag gctactagag cgacaccgct gctcttgttg gggtgctcgc tccgggcttc
    15301 cggcaacaga tgcgcggccc ctacgtaggt cagcacgcct ccggccatgc ccgtcagtag
    15361 acctagcgtc ccgctacccg gcaccggcag gagcaccgta agtccggcgg cgagcgggat
    15421 cgcgagacct aacagagccg tcgtgcggag aacgcgagat cggctcgcgc cactggcgac
    15481 gaagacggcg gccagcgaga tccccaccgg gagctgatgt atcacaattc ccagcccgac
    15541 cagtcccgac gtgacctcca cggtccgcgc gccgactccc agcacgaatc cgtcggcgag
    15601 gttgtggatc atcaatccca ggacgaaaga tcccagcatg tgcctgggga cgccttcttc
    15661 ggggacgtgg tgcgtgtgcg cgtgtgtgaa caactcgata acgaagagca gcgcaaaccc
    15721 tccgacgaag ccgagtatgg ctgcatccct ggcaagctcc agtccctccg gaaagaggtc
    15781 cgcgaacgcg agcgcgagga gtatgccggc cgcgaccgcc gccggatagg agcgacccgc
    15841 gaacatggtc ttccgcccga gcagcccgag agagctggaa accatgaacc cggccgcggc
    15901 gatcagcgcc agaagaaagg cggtcacggg gtccacccgc tctccgcgcc ggaagcgcgc
    15961 cggaagcgcc catctatctg caagtcgcgc accgtccgta cacctcgacg ctgtgggagc
    16021 gcacctcgaa cccaacagcg ccagggtcca gaagccgagg atctagggga caaagatcga
    16081 actccgtgat ctctccgcac ccctcgcaga ttaaatggtg atgatggtcc tcggctaact
    16141 cataccgcgg cctgtcgccc aggtccagac gccgcacgag cccaagctcc gagagcagat
    16201 ccagcgtccg gtacaccgtt accatcccca cacccggaca ccgcgcccgg atcccctcca
    16261 tactctgatg ctgctccgcc gctaccgccc gcactatcgc ctcccgctgc ggcgtaacct
    16321 tgaaaccccc acctcgcaac ttcccgacaa ccgccccgac gtccaacctc tctccttcta
    16381 aatgaaaaaa tttttcactt acttgaagag gttagcagag gagtgtggcg cgttcaaggc
    16441 gaaggtcttg aggtatcagg gaggtgtcag ggagatgtca gggagatgtc gggcgtttgc
    16501 ggcccgagcc ggagtggcgg aggaggagga tcatggcgaa gccgagggcg agggcgctgc
    16561 tcacgagcag gttgccccgg atggtgccgc cgatgaggaa ggcgggatct atcctcagcg
    16621 tctccacgaa gaagcggccg acggagtaga ggctgacgta gaggaagaag atgtcgccgt
    16681 ccttcaggcg ctcggagaag cggcgggcga cccacaggag gatcaggcac acgatcacgt
    16741 tccagatgca ctcgtagagg aacgtcgggt ggaacgaggt agcgtcggag taacctacgg
    16801 gccggttctc cggagctatc cggatagccc acggcaggtt cgacgggcgt ccgaacagtt
    16861 cctggttgaa gtagttcccc caccgtccga tagcctgggc caggatcacg cccggcgccg
    16921 ccgcgtcggc gaaggccagc acgctaatgc cgcggatgcg ggcgaagatc aggagcccga
    16981 ggagcccgcc gacgacggcg ccgtagatcc caagtcctcc attctggacc atgaagacag
    17041 ccgggaacgg gtcgtcccgg tagagttcgt agtccgtgat tacgtggtag agacgggcgc
    17101 ccacgaaacc gagcggcacc aggaagaata gcgagtccag ggccagagcg cgatcgtagc
    17161 ctttccgctc cagttcccgg cccgccagcc acgtggcggc tacgatcccg agggcgatga
    17221 acaacccgta gtagcgcagg gggaacgggc cgatctggaa tatgatcggc gacggcgggc
    17281 ttggcagaga gagtagagcg aaagtcatgg cgaccaacct taccttattg ggccggcgtt
    17341 ccagcatagg tggagaccag gccggagtcc tgctgcgcgg cagcatcctc gacgccgtag
    17401 cgggcttcca ggatcttacg caccgcgggc gccgccgtaa cctcgctgtg tccgccaccc
    17461 tcgaccatca ccactactac cagcgggtcc ttctcattct cggcccatcc ggcgaaccag
    17521 ttcaccgggt ccttgttgcc ccgctcgccc gtgccgctct ttccgacgat ggagagcttc
    17581 gagtcctcga aggccggggc ggccgtaccc tcagggccgg tcaccccgcg cagacccttc
    17641 agcaactcgt ttcgggccga ttcgttgaac tcgatcttac cctcgggttt cggggagatc
    17701 tcctgcttct tgccatcgcg gtcggttatc gctttggcga cgtgcggggt tacgagagtt
    17761 ccgtcgttct ccacggctga gtaggcctgg gccatctgga tcggactgac caggatatct
    17821 ccctgtccaa tcgagaggtt tatccagtcg gcgatccccc acggacggtc cagatatccg
    17881 tagacttctt tctgatacga ctcgaaccac tgtttgtccg ggatgcggcc gggagtctcg
    17941 ccagggagat ccacgcccgt cgtcgagccg aacccgaagc gcttgtagaa gtcgggcagc
    18001 aggttctcgt cgttggtctt ctgccagagc cagtccgcca cctggtagaa gaacttgtcg
    18061 ttggagtccg cgatagcctc ggcgtagttc tgggtcccgt gcgtcgagcc cgtcccgtaa
    18121 gactgccgcc aggagtacca gcaactgccc gtcgtcccag ccggctcgaa gcacccgccg
    18181 ttgtccgtta cggtcgtcgc cgcgtccagg acgccatagg cgaaccccgc caaccccgtg
    18241 aagaccttga acgtcgaggc gccgggatac gctcccgcta tggcgcggtt ggtgaacggc
    18301 gagttggcgt actcggagtt gaggtacttg aacgtctccg cctcgtcagc cccggagatt
    18361 ccccccacga agagttgcgg gtcgaaagtc ggacggctcg ccatcgccag tatctccccg
    18421 tttcgcgggt ccatggcgat tacggccccg ccctcgcccg agtaaccctc ctgctgggcg
    18481 cggactatgg ccgcgtcgag ctccgactcg gcggtcttct ggagtttcat atcgagggtg
    18541 agcgtgaggt ccttgccggg ctgcgggtcc gtgatgctcg cgggacgtcc gagttccggg
    18601 gagacctccg gcgctccgtc ggcgcgggtg ccgtccgcgc gccggacggt gaccacccgt
    18661 cccacggcgt ccacgttgta cttcttgctg cccggctcgc ccctcaactc gttctcatac
    18721 gtcagctcca cgccgctctt accgacgacc gagtcgttgg agaggccctt gaagtgcttc
    18781 tgcttcaact cctccgcggt gacggcgccc gtgtagccga ggacgtgcgc ggcgagctcg
    18841 ccctgcgggt agttgcggac gaagtcgtcg gtaacggtta cgccgcggaa ctcgttggtt
    18901 cgctccatca cgtaagtgac cgcgtcccgg tcagcgttct ccttgaccag catcgagcca
    18961 tagcggttgc ccgtctcggg gtccatcgcg gcgttgtagc ggtcggtcac cgccttcacg
    19021 tcggcctgca tgatcttcgc gagcttctgg accttctcgc gcgatatctc gttcgggata
    19081 acggtaacgt tcaggccggg gatgttgttc gcgagcacgt ctccgttgcg gtccttgatc
    19141 acgccgcgct gagccgggat cttgacctcg cgggtgtgcg tctgctgggc tgacttggtg
    19201 aagttctcgc tcgtgaggac ctgcaggtac cacagccggg tccccaggat ggcgaagagc
    19261 acggcaacga gcaccgcgat ggtcaccacc cgcacctgca tgttgccctt agtcgccggc
    19321 ggcgtgacgc cgcgtttttt ggcggggaca ggctcatggt gcctgcgctt cggtacgcgc
    19381 agcaaggcct aggccaccct ctccgcctgg acgatcaggc gctgcgagta atccggcagg
    19441 gtgcaggtct gcaaggtgac tatattctta ttcttaaccg ggcgggttat gtagacgtcc
    19501 gaagggtcaa caacgaagtc cttgaatacc ttgtatacat atcgcctgcc catcgaatcg
    19561 gtgataaaga tcttgtcccc ctctgtgagc acgttcaggt cgtagaacgc gagaaagctc
    19621 ttcgtgttca ggtagcccag cctgtgaccg gcgatgtaga cgttcgcctc cttctcccag
    19681 gggaacccgg tccccttgag atggatggcc gtatggtcgt gcagagcctg ctcatcatcg
    19741 cccgtagtgt agggtatatc cgtgtcgtcc acccgcgcca tcttggggat ggtgatcctg
    19801 agcgtcttgt cctttggtac gggaatcgcg ggctcgggct tcttctcctt gtccttctcc
    19861 gccggggccg ccggcgcgat ggcgggctcc ttctgagccg actcgtcgag gacggggacg
    19921 ttgaactcgt ccttgctcac gctggaagac tcctgcgcgt tgccggagga gacgaaagga
    19981 gacccgaaga agaacgtcgt gataagcgcg agccctgcta ccaccagtac gaggctcatc
    20041 aaattcaggg ctatgttcgc aaaacgcact ctaagttatc ctccgcgagc gagccggtat
    20101 tctcctgaga acctctcctg gatcgcaacc cattgtatcc gaaaacaact tgacgactag
    20161 cctttcgctc cgggtctttg caaactatcc ccgaacgacg aatagcttcc tggcgacctc
    20221 gctatcctgt acgtttgccc ccgcgcgccg gattatgacc gtatagttgc cggccggcaa
    20281 ggactccccc gatcgcgtcc tgaccgcgaa cttgacgacg cccgccacgc ctctggaagc
    20341 tggcgagagc tggtcctcct gtccgtccac cacctcgata tccgccgcgc tggagaacag
    20401 ccggcccagg acggactcgc gcccggcgcg gtccacgcgc gcggtgagtt tcgtcccgga
    20461 aggcagtcca tctaccgcaa cgtaaacgta gatctccgcc ggcgcctcgc tgaaccggag
    20521 gacgtccgag ggcgggtaga gcgtgtcctc ctcgctcacc gccacgtcct tgataccctc
    20581 ggcctccatc ttctcgcccg gctcgaatcc tcttccggcc aaaagcgccc acacgagcgc
    20641 caccagaacg actatcgcca gccctccgag cagatacccg gaggcgcgcc ggggtgcagg
    20701 atctcttttc ttcgtcgtcg gcagcgcggt cacggcctct tctcagatct cgcgcgcttc
    20761 cagaccagga aagtagctcc gttgtgcagc gtgtgggcta taaccggcgc agcaatgccg
    20821 cccgtcgcga taaacagcgc gccgaagaga aacccggccg ctacggccca gaccgtccag
    20881 atcacgaacc tgcggtcggg accgacgtgc aagagcccga aagccaggga ggcgatcacg
    20941 atcccaaact ccggctgcac ggcgccgcga aagaagacct cctctccgat accggagaag
    21001 accgagagca agaccagatc ccgccgcgag gtcccgtcaa ccagcatcgg cgccagctcg
    21061 tctgccagcc ggcgcagcaa ggggacggcc cggtacgtca gcagacccgc gctcacggtg
    21121 acggccgcgg ccagaacgcc gagcgagacg gattcgggca ccgagtctcc gacggcgaaa
    21181 agggcccggt cccgaaggcc gttccacagg gcggcggcga cgagtaggaa cccgtagaag
    21241 agggctgctt gcttaacgaa gcgcggtccc aagcggaacc tctcgggcct ctagcgtagg
    21301 ctggactgca agcgcgatca taacccgcta ttgtaccgga gacgaattcg ggtggtctct
    21361 cggcttcccg gactcgtgga gatccgtagg aagaaaggtg cgatgataga cgagtccata
    21421 ttcaaggcct acgacatccg cggcgtctat cccgactcg
//
LOCUS       DASSMB010000297        12569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1741418, whole genome shotgun sequence.
ACCESSION   DASSMB010000297 DASSMB010000000
VERSION     DASSMB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12569
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741418"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /gene="nuoB"
                     /locus_tag="VFJ72_16855"
     CDS             complement(<1..539)
                     /gene="nuoB"
                     /locus_tag="VFJ72_16855"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HET7481178.1"
                     /translation="MQQFKEPNIVTTSTDKLFNWARKNSLWPLQFGLACCAIEMMSTG
                     MAHNDLDRFGAGFFTASPRQADVMIVSGTVSTKMAERMTRLYEQMPEPKWVIAMGACA
                     ISGGPFLDGYSVLMGADRVIPVDVYVPGCPPRPEALIFGIMTLQKKIERDQQVGYTRP
                     RPALVDEDGTVMEYLPERE"
     gene            complement(539..862)
                     /locus_tag="VFJ72_16860"
     CDS             complement(539..862)
                     /locus_tag="VFJ72_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET7481179.1"
                     /translation="MLLITGFVATVLVLARLISPSRRSRTKAQIYETGESTVTDPWRP
                     FPVRYYVFALLFLIFDVEAAFLYPWAVIYKSLGVYGFVEMVIFVVILGVGLAYAWKKG
                     ALKWV"
     gene            complement(1026..2096)
                     /locus_tag="VFJ72_16865"
     CDS             complement(1026..2096)
                     /locus_tag="VFJ72_16865"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7481180.1"
                     /translation="MRYKSYTPGVAQSAMDFISGWFEARGLDPMQYKGAGPGGELTSL
                     VVSVGSGGPKILLHGHVDVVPGEEWQFEPHEEDGELYGRGVYDMKGALAAMMYAVHDL
                     NETGCDCTVELLVVPDEEHEYEGPTGAEILIRNGHVGDFMITGEPTDFHVGTQAKGVL
                     DLRITLLGKSAHGSRPWLGKNAVLLAYEHFRRVGELPFATERSELFPYPSINLARIIG
                     GDVINRVPERCTYDLDIRYLPNQDPREIVRQIRSIELPAEVEVLFSREPTYVSRRNPY
                     VKALREVAARHFHGNPVGVGRHGASDIVYFQRAGVPGVEFGPIGGGHHGPREYVVTDS
                     LETYRQMLVQFVQILGRNGKGH"
     gene            complement(2261..3547)
                     /gene="murA"
                     /locus_tag="VFJ72_16870"
     CDS             complement(2261..3547)
                     /gene="murA"
                     /locus_tag="VFJ72_16870"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET7481181.1"
                     /translation="MERFLIEGGARLEGTVEISGAKNAALKFIAASLLAPGRTTLGNV
                     PRIKDVDTIRAVLEGLGASTDFSEGILKIDAAEIESYTAPYELVRQMRASIVVLGPLV
                     ARFGEAEVSAPGGCNLGLRKFNFHLDGLRALGADVELDHGFIKATAPNGLRGAEVNFE
                     YPSVTATENVMMAAVLAEGMTIIENGAREPEVVDLAEFLNAMGARIQGAGSGRIVIEG
                     VEELRPVEWTIMPDRIEAGTFIMATAAAGGDVTVRNARPEYLKMEFEKLRETGLDLRL
                     LDDGVRVQVDDPRELHGVDVSTLPFPGFATDLQAQMMVLLSQVSGAGIITENVYENRL
                     QVAEELNRMGAGVDLFGGHRALVRGPRRLSGTIVQSPDLRGGAALVMAGLVAEGTTVV
                     DGAKHILRGYENFEEKLTGLGATVAFESRAGTTVST"
     gene            complement(3589..4047)
                     /gene="atpC"
                     /locus_tag="VFJ72_16875"
     CDS             complement(3589..4047)
                     /gene="atpC"
                     /locus_tag="VFJ72_16875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HET7481182.1"
                     /translation="MAESREERSEGRQLFCRVITPEEVVYDGEVDLVIARIADGEIGV
                     LVDHAPLVSSLEFGDVRIKEGEEQHVYATSDGFFKVSENLVQILVEEAVPPGEIDIDE
                     AETRIEEAERELAELADQDEEERKPVEREIERRRRMGENLARSARRYGEG"
     gene            complement(4047..5765)
                     /gene="atpD"
                     /locus_tag="VFJ72_16880"
     CDS             complement(4047..5765)
                     /gene="atpD"
                     /locus_tag="VFJ72_16880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7481183.1"
                     /translation="MSESGQEFNRTTFGNREGGGIEGGQVNGGDGQPTEQRTHQNGHD
                     GRGSGVGTVVEIKGPVVDVRFSPEDIPETYNALTVPIGGGEGKEVSQLTLEVQQLLGD
                     DMVRTVAMSSTDGLQRGLDVTDTGQPIAVPVGKSVLGRVLDVLGQPVDEQGPVEDPED
                     YWPIHRPSPKFEDLTTQAEQFVSGIKVIDLLCPVRRGGKVGLFGGAGVGKTVVITELI
                     NNIALKYEGTSVFAGVGERTREGNDLYGEMEEAGVLPNMAMVFGQMNEPPGARFRVAL
                     TGVTVSEYFRDELGQDVLFFVDNIFRFVQSGNEVSALMGRMPSEVGYQPTLGTEMGAL
                     QERITSTKNGSITSFQAVYVPADDYTDPAPFTVFAHLDSTVELSRGLAEQGIYPAVDP
                     LTSSSTMLDPSIVGDEHYEVAQQVKNILQRYKELQDIIAILGIDELSEEDKVIVGRAR
                     RLQRFLSQPFFVAEQFTGLEGKFVELDETIRSFKEVVEGKHDDLPEQAFYLVGNIDEA
                     VEKARKIGGDEDGEDGQAEDETSAEAEEDAGSQASAEDESPVAESGSEQSDEESGSGD
                     QEEQKS"
     gene            complement(5782..6651)
                     /gene="atpG"
                     /locus_tag="VFJ72_16885"
     CDS             complement(5782..6651)
                     /gene="atpG"
                     /locus_tag="VFJ72_16885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HET7481184.1"
                     /translation="MATLRDLKRQIGSVKNIAKVTEALQTVSAVKFRKAEARVKKARP
                     YAENVEQMMRDVARSATGSSPLLAGREVNTVLIASITADRGLAGGFNAQILRRTMQFR
                     NEQSAELKQVASGRKSVSFFRFRRVGLEEVYTGFSDSPTYEKAREIGQALVRLFDDEE
                     ADEVYLIYNRFETALTQKPVIKRLIPVAPEEEEDEEERDDSVSYMEYVPDAETVLKRL
                     VPRYVETMVWQALLESAAGEHGARMTAMKSATDNANDLAKSLTLQMNKARQAQITSEI
                     SEIAAGAEALSAG"
     gene            complement(6655..8289)
                     /gene="atpA"
                     /locus_tag="VFJ72_16890"
     CDS             complement(6655..8289)
                     /gene="atpA"
                     /locus_tag="VFJ72_16890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET7481185.1"
                     /translation="MGRLRPEEISSILKGAITDYENRVRTEEVGQVLEVGDGIARVHG
                     LTNAMYQEMLEFEDTRGNKVIGLALNLEEDNVGVIIAGDDTHIQEGNTVRRTERLASV
                     PVGEGVLGRVITALGVPMDGKGEIEAEDTLPVEIVAPGIIKRQPVKTPLQTGIKAIDA
                     MVPIGRGQRELIIGDRKTGKSAIAIDTIINQADQNVKCIYVAIGQKDSTVAGVVSALE
                     EAGAMEFTTIVNASAAQSATLQYLAPYAGCAIGEYFMNKGEDALVIYDDLSKHAVAYR
                     QMMLLLRRPPGREAYPGDVFYLHSRLLERAARMSDAAGGGSLTALPIIETKAGDISAY
                     IPTNVISITDGQIFLDSDLFNSGQRPAIDTGNSVSRVGGSAQIKAMKSVAGTLRLDLN
                     QYKELQSFAQFGSDLDRATQQTLNRGERLTAVLKQEERDPLPVEEQVVVIYAASNGYL
                     DEVEPDNVPDWERQFRDHLKDSHGEILDSIRDEKQISDETKKKLNEVVERFSENYEPE
                     RESLVNVTAGGDAGADEGATGDEPRGEDKNASGERA"
     gene            complement(8290..8829)
                     /gene="atpH"
                     /locus_tag="VFJ72_16895"
     CDS             complement(8290..8829)
                     /gene="atpH"
                     /locus_tag="VFJ72_16895"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HET7481186.1"
                     /translation="MSAVSTYAGALFEAAKERDELEGVLEDLREFVDALRESEELRLF
                     FYGAQIPERQKRKALEGLSEGMKPTTTNFLKVLVDNGREHIIGDVLRNYEDLVKEHLG
                     RIEVEVTTAVELSEESRERLRERMGKILDGREVVLETRVDPDLLGGAVFRFGGQMMDG
                     SVRGRLESLREGMFERSVV"
     gene            complement(8826..9401)
                     /gene="atpF"
                     /locus_tag="VFJ72_16900"
     CDS             complement(8826..9401)
                     /gene="atpF"
                     /locus_tag="VFJ72_16900"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET7481187.1"
                     /translation="MSSIDPKGIFDLPNTSLIFWTVITFLILLFLLIRYVYPPIRDHI
                     LKRQSEIERSIDEAEMTRKEARELLAEYRRQLDEARGESRRILDDARKQAEAQRERTK
                     KEAREEGDKIIQRAREEIGRERESALREVRREVAGMVIQASERVIGREVNQDEHERLI
                     SQALDDLEAEVAGTPTGSAAGTATGNGGRTA"
     gene            complement(9435..9692)
                     /gene="atpE"
                     /locus_tag="VFJ72_16905"
     CDS             complement(9435..9692)
                     /gene="atpE"
                     /locus_tag="VFJ72_16905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HET7481188.1"
                     /translation="MLEMLALLQSSSEGLKLVGTGIAAGTAVIGPGAGMGYLIGQTIA
                     SIHRQPEAFEQTRTLMFLGIGLVEAFALYGIVFALLIAFVL"
     gene            complement(9728..10510)
                     /gene="atpB"
                     /locus_tag="VFJ72_16910"
     CDS             complement(9728..10510)
                     /gene="atpB"
                     /locus_tag="VFJ72_16910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET7481189.1"
                     /translation="MLALMEVTQEELREKILHTFKSGKDAWEIPLHLGPIDISITKAV
                     WFLFFGAAVTFLILFIGSRLLKDRPGAYQVIVEEIYGFGRNLGGQVGEEGRKWFPYTL
                     SLFVFILVLNLIGLIPNTYPVTANISFTLMLAILTFAITQFEGFRRYGAVTYLKSFAP
                     PGLPAKPLMVPLMWFIEVLGEFTKPLTLGMRLYANILAGHLIIYVFLGLILFFGSFVA
                     LISVPLSVVFYAFEIFVAVLQAYIFAILTQVYIELAMFREDH"
     gene            complement(10516..10944)
                     /locus_tag="VFJ72_16915"
     CDS             complement(10516..10944)
                     /locus_tag="VFJ72_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481190.1"
                     /translation="MSGHWRVAIRCAAWMIVLAAAAAGAIFYVYGPDHSLAFCYGAGV
                     GIVSFASTAITVSLLTGRSRVIGVLLGATSFGVRYGFAAVALGIPAYLELWPVVAMLM
                     GFTAVYFVETLVLVPWAVRAMGVPGERRPAGEAVERRIEA"
     gene            complement(10941..11168)
                     /locus_tag="VFJ72_16920"
     CDS             complement(10941..11168)
                     /locus_tag="VFJ72_16920"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HET7481191.1"
                     /translation="MPHTDSQGNRSGGSYGRFIGLGFTLILIFALPTVVGFFVDKFAG
                     TLPLFLLLGLALGFVGGLYYVYRALQRLDGG"
     gene            complement(11263..12138)
                     /locus_tag="VFJ72_16925"
     CDS             complement(11263..12138)
                     /locus_tag="VFJ72_16925"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7481192.1"
                     /translation="MAVTRVSGGMREVRLEQGVIRYRDAGSGPALVFVHGILVNGTLW
                     RHVVARLSDKFRCIVPDLPLGSHSIPLGADADLSPKGVARIVADLIETLNLEDVTLIG
                     NDTGGAICQIVIAEHSDKISRLVLTNCDAYEAFFPLLLRPFQYAARIFGVRFVDALAW
                     LLRARAAQRGLFWTVSKRRQDTRTLDAYIGPLISDAGVRRDLTRYLQGVSNRYTIDAA
                     RSFREFHKPVLLVWGEDDIFFPPRYAERLRRDFPAAVLKRVSDSRLRPGRSATVVGRA
                     HRSLRRAAGKRWGVR"
     gene            12271..>12569
                     /locus_tag="VFJ72_16930"
     CDS             12271..>12569
                     /locus_tag="VFJ72_16930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7481193.1"
                     /translation="MSERPRRRHTSQQAERAILAAARSFLAEHPFRELTAEAVMTRTG
                     LSRTAFYAYFRDRYDLVTRLLEGIGALLFAVDRYWLEGGEEAGAPASESLGEALRR"
BASE COUNT         2246 a         4230 c         3714 g         2379 t
ORIGIN      
        1 tactcccgct ccgggaggta ctccatgacg gtgccgtcct cgtccacgag cgccggacgc
       61 ggcctcgtat agccgacctg ctggtcgcgc tctatcttct tctggagcgt cattatgccg
      121 aagatcaggg cttccggacg cggcggacaa ccggggacgt acacgtccac cgggatcacg
      181 cggtcagccc ccatgagcac cgaatatcca tcgaggaacg gaccgccgga gatggcgcag
      241 gcgcccatcg cgatgaccca cttgggctcg ggcatctgtt cgtagagccg ggtcatccgc
      301 tcggccatct tggtcgagac ggtcccggaa acgatcatga cgtccgcctg acgcgggctc
      361 gcggtaaaga acccggcccc gaagcggtcg aggtcgttgt gggccatgcc ggtggacatc
      421 atctcgatgg cgcaacacgc caggccgaac tgcagcggcc acagcgagtt cttgcgggcc
      481 cagttgaaga gcttgtccgt gctcgtcgtg acgatgttcg gctctttgaa ctgctgcatt
      541 acacccattt aagggctccc ttcttccagg cgtaggcaag tccgacaccc agtatcacga
      601 cgaagatgac catctcgacg aaaccataga cgccgaggct cttgtagatg accgcccacg
      661 ggtaaaggaa agcggcctcc acgtcgaaga tcaggaacaa gagggcgaag acgtagtagc
      721 gtaccgggaa cggacgccag ggatccgtga cggtggactc tccggtctcg tagatctgag
      781 ccttcgtccg ggacctgcga ctcggcgaga tgagccgggc gagaaccaga acggtcgcca
      841 cgaagcccgt tatcaacaac atgaagaccc caacgtacaa gtacaatgtc gtgtacgcca
      901 tttgctgctg aattagaccg agcgccaaac taaacctttc ttcccggatg acactcgcaa
      961 tatactcgtg ttcagaaaaa ctttcaagac gctactaaca gtagcaaatc tcacttaatg
     1021 ttcttttaat gtcctttgcc gttgcgcccc agtatctgca cgaactgcac gagcatctga
     1081 cggtaggttt cgagcgaatc ggtcaccacg tactccctgg gaccgtggtg tccgccgccg
     1141 attgggccga actcgacacc gggcacaccg gcgcgctgga agtagacgat gtctgaggcg
     1201 ccgtgacgtc cgacgccgac ggggttgccg tggaagtgac gggcggcaac ctcgcgcagg
     1261 gccttcacgt atgggttgcg gcgggagacg taggtcggct cgcgggagaa caggacttcc
     1321 acctccgccg gcagctctat cgaccggatc tggcgcacaa tctcccgcgg gtcctggttc
     1381 ggcaggtacc ggatgtcgag gtcgtaggtg caacgctcgg ggacgcggtt tatgacgtcg
     1441 ccgccgatga tgcgggccag gttgatgctc gggtacggga agagctcgct cctctccgtc
     1501 gcgaacggga gttccccgac gcgccggaag tgctcgtagg cgaggaggac ggcgttcttg
     1561 cccagccagg ggcgcgagcc gtgggcgctc ttcccgagga gcgtaatccg caggtccagc
     1621 acgcccttcg cctgcgtacc aacgtgaaag tcggtgggtt cgcccgtgat catgaagtcc
     1681 ccgacatgcc cgttacggat caggatctcc gccccggtcg gtccttcgta ctcgtgctcc
     1741 tcatccggca ccacgagcaa ctcgaccgtg cagtcgcacc cggtctcgtt caggtcgtgg
     1801 accgcgtaca tcatcgcggc caaagcgccc ttcatgtcat agacgccacg accgtagagc
     1861 tcgccgtcct cctcgtgagg ctcgaactgc cactcctcgc cgggcaccac gtccacgtga
     1921 ccgtgcagaa ggatcttcgg tcccccggac cccacgctca cgacgaggga ggtaagctcg
     1981 ccgccgggtc ccgccccctt gtattgcatg ggatccaggc cccgagcctc gaaccagccg
     2041 ctgatgaaat ccatcgccga ctgggccacg cccggagtgt aggacttgta gcgcacgagg
     2101 cgccgggtga gcgcgaatac ttcggtggtg tgtgccttta ccgccagccc aggtctccgt
     2161 ccagctagtc cactgtatac aaagagcgta ttctatatta tgcggcgcga aagatcgccg
     2221 gtagacgcgg caaagggtgc gcagttttac gtggatacga ttatgtggat acggtcgtcc
     2281 cggcccggct ctcgaaagct acggtcgccc cgagcccggt gagtttctcc tcgaagtttt
     2341 cgtatccgcg cagtatatgt ttcgcgccgt ccacgaccgt cgtgccctcg gccacgagac
     2401 cggccatgac gagtgccgcg ccgccgcgca ggtcaggcga ttgcacgatg gtgccggaga
     2461 gcctgcgggg gccgcgcacc agagcccggt gaccgccgaa caggtcgacg cccgcgccca
     2521 tccggttcaa ctcctcggcg acctgcagcc ggttctcgta gacgttctcg gtaatgatcc
     2581 ccgcccccga cacctgcgag agcagaacca tcatctgagc ctgaagatcg gttgcgaagc
     2641 cgggaaaagg cagcgtagag acgtctacac cgtgcaactc cctggggtcg tccacctgca
     2701 cccggacgcc atcatccagc aaccgcaagt ccagccccgt ctcccgaagc ttctcgaact
     2761 ccatcttcag gtactccggc cgcgcgttcc tcacggtgac gtcgccaccg gccgcggcgg
     2821 tcgccatgat gaacgtgccg gcctcgatcc ggtccggcat gatagtccac tcaacgggcc
     2881 gcaactcctc gacgccttcg atcacgatcc tgcccgaacc cgcgccctgg atgcgggcgc
     2941 ccatggcgtt gaggaactca gcgagatcca cgacctccgg ctcccgtgcg ccgttctcga
     3001 taatcgtcat gccttcggcc agtacggcag ccatcatcac gttctccgtg gccgtgacgc
     3061 tcgggtactc gaagttgacc tccgccccgc gcagcccgtt cggcgcggtc gccttgataa
     3121 agccgtggtc caactccacg tcggcgccca gagcccgcag gccgtccaga tggaagttga
     3181 acttccgcag acccaggtta cagccgcccg gagccgagac ctccgcctcg ccgaaccggg
     3241 ccactagggg tcccagcacc acgatagagg cccgcatctg gcgcaccagc tcgtaaggag
     3301 cggtgtagga ctcaatctcc gcggcgtcta tcttgaggat accttccgag aaatccgtac
     3361 tcgccccgag cccttcgagg acggcccgga tagtatctac gtccttgatc cggggtacat
     3421 tgccgagcgt ggtgcgtcca ggcgccagaa gtgacgcggc gatgaatttc aaggcggcgt
     3481 tcttagcgcc ggagatctct accgtgcctt cgagacgcgc tccgccctcg atcaggaacc
     3541 gctccatcaa tatcctccca gaagtagatt cgggcctagt ttacccgatc aaccctcgcc
     3601 gtaacggcgc gccgaccggg ccaggttctc gcccatgcgg cggcggcgtt cgatctcccg
     3661 ctccaccggc ttcctctcct cctcgtcctg atccgcaagc tccgccagct cgcgctcggc
     3721 ctcttcgatc cgggtctcag cctcatcaat gtcgatctca cccggcggaa ctgcctcctc
     3781 gaccaggatc tgcaccaggt tctccgagac cttgaagaag ccgtccgagg tggcgtagac
     3841 gtgctgctcc tcgccctcct tgatgcgcac gtctccgaac tccaggctcg acaccagcgg
     3901 cgcgtgatct accaacaccc cgatctcacc atccgctatc cgggcgatga cgagatccac
     3961 ctcgccatcg tagacgacct cctcgggcgt tatgacccgg cagaataact gccgcccctc
     4021 actccgctct tcgcgactct cggccactag ctcttctgct cttcttgatc gccagagccc
     4081 gactcctcat cggactgctc gctgccggat tccgccacag gagactcgtc ctcagccgag
     4141 gcttggctgc cagcatcttc ttcggcctcc gcggatgtct cgtcttccgc ctgaccatct
     4201 tccccatctt catcgccgcc gatcttgcgc gctttctcca cggcctcatc gatgttgccg
     4261 acgagataga aggcctgctc gggcaggtca tcgtgcttgc cctcgacgac ctccttgaac
     4321 gaacggatcg tctcatccag ctccacgaac ttgccttcga gcccggtgaa ctgctcggcc
     4381 acgaagaacg gctgcgagag gaaccgctgc aggcgccgcg cccgtccgac gatgaccttg
     4441 tcctcctccg agagctcgtc gataccgagg atggcgatga tgtcctgaag ctccttgtag
     4501 cgctgcagga tgttcttgac ctgctgcgcc acctcgtagt gctcgtcgcc gacgatggac
     4561 gggtcgagca tcgtggagga tgaggtcagc gggtctaccg caggatagat accctgctcg
     4621 gccaggccgc gagacaactc caccgtggag tcgaggtgag cgaacaccgt gaacggggcc
     4681 gggtcggtat agtcgtccgc cggcacgtat acggcctgga agctggtgat ggagccgttc
     4741 ttggtcgagg tgatgcgctc ctgcagcgcg cccatctcgg tcccgagcgt cggctggtaa
     4801 ccgacctcgg agggcatccg gcccatcagg gcggagacct cgttgcccga ctgcacgaag
     4861 cggaagatgt tgtccacgaa gaagagcaca tcctggccca actcgtcccg gaagtactcg
     4921 gagacggtca cgcccgtgag cgccacccgg aagcgcgcgc ccggcggctc gttcatctgt
     4981 ccgaagacca tcgccatgtt cgggaggacg cctgcctcct ccatctcgcc gtagaggtcg
     5041 ttcccctcgc gggtgcgctc cccaaccccg gcgaagaccg aggtgccctc gtacttgagg
     5101 gctatgttgt tgatcagctc ggtgataacc accgtcttac ccacgcccgc gccgccgaac
     5161 agtcccacct tgccgccccg gcgcaccgga cagagcaagt cgataacctt gatgcctgat
     5221 acgaactgct cggcctgcgt cgtcaggtcc tcgaacttcg gggaggggcg gtggatcggc
     5281 cagtagtcct cggggtcttc taccggaccc tgctcgtcaa cgggttgtcc cagcacgtcg
     5341 agcacgcgac cgaggacgct cttccctacg ggcacggcta tcggctggcc cgtgtcggtc
     5401 acgtcgaggc cgcgctgcag gccgtccgtg gaggacatcg ccacggtgcg gaccatgtcg
     5461 tcgccgagga gctgctgcac ctccagggtc agttgcgaca cttccttgcc ttcgccgccg
     5521 ccaatgggga cggtgagggc gttgtaagtc tccggtatgt cctccgggga gaagcgtacg
     5581 tccacgacgg gacctttgat ctccacgacc gtgcccacgc cgctgccgcg tccatcgtgg
     5641 ccgttctgat gcgtgcgctg ctcggtgggc tggccgtcgc cgccgtttac ctgtccgccc
     5701 tcgatgccgc cgccttcgcg gttgccgaag gtagtccggt taaactcctg gccgctctcg
     5761 ctcatagaaa agtacctcct gctatccggc ggacagggcc tcggcgcccg ccgcgatttc
     5821 gctgatctcg ctcgtaatct gcgcctgccg cgccttgttc atctgcaggg tcaggctctt
     5881 ggccaaatcg ttggcgttat ccgtggcgct cttcatcgcc gtcatgcgcg ccccgtgctc
     5941 gccggccgcg ctctccagca gcgcctgcca caccatcgtc tccacgtacc gcggcaccag
     6001 ccgcttgagc accgtctccg cgtccgggac gtactccatg taggagacgc tgtcgtctcg
     6061 ctcctcctcg tcctcttcct cctctggagc cacgggtatg agacgcttta tcaccggttt
     6121 ctgagtgaga gccgtctcga agcggttgta gatcaggtac acctcatcgg cctcttcgtc
     6181 gtcgaagagc ctgacgaggg cctgcccgat ctcacgcgcc ttctcgtagg tcgggctgtc
     6241 ggagaatcca gtgtagacct cttcgagccc gacgcgccgg aaccggaaga acgacacgga
     6301 cttccggccg ctcgcgacct gctttagctc cgcgctctgc tcgttacgga actgcatggt
     6361 gcggcgcagt atctgggcgt tgaacccgcc cgcgagaccc cggtcagccg ttatcgaggc
     6421 tattagaacc gtgttgacct cacggccggc gaggagcggg ctgctgccgg tcgcgctgcg
     6481 cgccacgtcg cgcatcatct gctcgacgtt ctcggcgtag ggccgcgcct tcttgacccg
     6541 cgcctcggcc ttgcggaact tcaccgcgga gacggtctga agcgcctccg taaccttggc
     6601 gatgttcttg acggacccga tctgccgctt gaggtcccgg agagtcgcca ctgtttacgc
     6661 cctctccccc gaggcgttct tgtcctcgcc ccgcggctcg tcgccggtcg cgccctcgtc
     6721 cgcgccggcg tcgccaccgg cggtgacgtt caccaggctc tcgcgttccg gctcgtagtt
     6781 ctccgagaag cgctcgacga cttcattcag cttcttcttg gtctcgtcgg agatctgttt
     6841 ctcgtcgcgg atggagtcca ggatctcgcc gtggctgtcc ttgaggtggt cgcggaactg
     6901 gcgttcccag tccgggacgt tatccggctc tacctcgtcc aggtaaccgt tcgacgcggc
     6961 gtagatcacc acgacctgct cttcgaccgg cagcgggtcg cgctcctcct gcttgaggac
     7021 cgccgtgagg cgctcgccac ggttgagcgt ctgctgcgtc gcccgatcca ggtcgctccc
     7081 gaactgcgcg aagctctgaa gctccttgta ctggttcagg tccagacgaa gcgtaccggc
     7141 gacgctcttc atcgccttga tctgggccga cccgcccacg cggctcacgg agttacccgt
     7201 atcgattgcc ggacgctgac ccgagttgaa caggtcggag tcgaggaaga tctgaccgtc
     7261 cgtaatggag atgacgttcg tcgggatgta cgccgagatg tctcccgcct tggtctcgat
     7321 gatcggcagc gccgtcagcg agcctccacc ggcggcgtcg gacatccgcg cggcccgttc
     7381 caggagacgc gagtgcaggt agaagacgtc gcccggatac gcctcgcgtc ccggcgggcg
     7441 cctcagcaga agcatcatct gacggtaggc gacggcgtgc ttggagaggt cgtcgtagat
     7501 cacgagcgcg tcctcgccct tgttcatgaa gtactcaccg atagcgcacc ccgcgtaagg
     7561 cgcgaggtac tggagcgtcg cggattgcgc cgccgaagcg ttcacgatgg tcgtgaactc
     7621 catcgcgccg gcctcttcca gagcggacac gacgcccgcc accgtggagt ctttctggcc
     7681 gatggcgacg tagatgcact tcacgttctg gtcggcctgg ttgatgatgg tgtcgatggc
     7741 gatggccgac ttgcccgtct tccggtcgcc gataatcagt tcccgctggc cccggccgat
     7801 cggcaccatc gcgtcgatgg ccttgatacc agtctggagc ggtgtcttga ccggctggcg
     7861 cttgatgatg ccgggcgcga cgatctccac ggggagggta tcctcggcct cgatctcgcc
     7921 cttcccgtcc atcgggacgc cgagcgcggt tatgaccctg ccgagcacac cctcgccgac
     7981 cggcacgctc gccagccgct cggtgcgccg cacggtgttc ccttcctgga tgtgggtgtc
     8041 gtcgccggca ataatcacgc cgacgttgtc ctcttccagg ttgagggcga gcccgatcac
     8101 cttgttgccg cgagtgtcct cgaactccag catctcctgg tacatcgcgt tggtcaggcc
     8161 gtggacgcgc gcgatgccgt ccccgacctc cagtacctgc ccgacctcct cggtgcggac
     8221 gcggttctcg tagtctgtga tagcgccttt tagtatggag gagatctcct ccggcctgag
     8281 cctgcccacc tacacaacac tcctctcgaa catcccctcc cggaggctct ccagccgccc
     8341 ccggacactg ccatccatca tctgtccccc gaaccggaag accgcgccgc ccaacaagtc
     8401 agggtctacg cgagtctcca gcacgacctc ccggccgtcg aggatcttgc ccatgcgctc
     8461 ccgaagccgc tcgcggctct cttccgaaag ctccaccgcc gtcgtgacct cgacctcgat
     8521 ccggccgagg tgctccttga ccaggtcttc gtagttccgc aacacgtcgc cgataatatg
     8581 ctcgcgaccg ttgtcaacga gcactttgag gaagttggtc gttgtgggct tcatcccttc
     8641 gctcagtccc tccagagcct tccgcttctg gcgctccggg atctgggctc cgtagaagaa
     8701 gagccgcaac tcctcgctct cgcgcagcgc gtcaacgaac tcccgcaggt cttcgaggac
     8761 gccttcgagc tcgtcccgct ccttcgcggc ctcgaagagg gctccggcgt aagtcgagac
     8821 ggcgctcaag cggtcctgcc tccgttgccg gtggcggtgc cggccgcgct cccggtggga
     8881 gtgcccgcga cctcggcttc gaggtcgtcg agggcctggg agatcaaacg ctcgtgctca
     8941 tcctggttga cctcgcgccc gatgacccgc tcggacgcct gtatgaccat cccggcaacc
     9001 tcgcgccgca cctcgcgcag ggcggactcg cgctcgcggc cgatctcctc gcgggcgcgc
     9061 tggatgatct tgtcgccctc ctctcgggcc tccttcttgg tgcgctctcg ctgggcctcg
     9121 gcctgcttgc gggcgtcatc gaggatgcgc cgcgactcgc cacgggcctc gtccaactgg
     9181 cgacggtact ccgccagaag ctcgcgagcc tccttccgcg tcatctccgc ctcgtctatg
     9241 gaacgctcga tctcggattg gcgcttcagg atatggtccc ggatcggcgg atagacgtac
     9301 ctgatgagca ggaagagcag gatcaagaag gtaattaccg tccagaagat caggctcgtg
     9361 ttcggtaggt cgaatatgcc tttcgggtct atgctggaca tacgctctcc tcctccctcg
     9421 tctccgaatg ccgcctagag gacgaaagct atgagcaggg cgaagacgat gccgtacagg
     9481 gcgaacgcct cgaccagccc gattccgagg aacatgagcg tccgcgtctg ctcgaaagcc
     9541 tccggctggc ggtggatgct cgcgatggtc tgcccgatca ggtaccccat ccccgcgccc
     9601 gggccgatga ccgccgtgcc ggccgcgatg ccggtcccga cgagcttgag cccctccgag
     9661 ctggactgca gtagtgccaa catctccaac atttccttgc tcctttcttt ccgttttctt
     9721 cgagcttcta gtggtcctcg cggaacatcg cgagctctat gtacacctgg gtaagaatag
     9781 cgaatatata ggcttgcagc acggcgacga atatctcgaa cgcgtagaac accacggaga
     9841 gcggcaccga tataagcgcc acgaagctgc cgaagaacag gatcaggccg aggaagacgt
     9901 agatgatcaa atgccccgcc aggatgttgg cgtagagccg catcccgagc gtgaggggtt
     9961 tagtgaactc cccgagcacc tcgatgaacc acatcagcgg caccatcagc ggcttggccg
    10021 ggaggccagg aggcgcgaag ctcttaaggt acgtgaccgc cccgtagcgc cggaagccct
    10081 cgaactgcgt tatggcgaac gttagtatgg cgagcatgag ggtgaacgag atgttggccg
    10141 tcaccggata tgtgttgggg atcagaccga tcaggttcag caccaggatg aacacgaaca
    10201 gggagagcgt gtaggggaac cacttgcgtc cctcctcccc gacctgtccg cccaggttgc
    10261 gcccgaagcc gtagatctcc tcgacgatca cctggtaggc accggggcgg tctttcaaca
    10321 gccggctccc gatgaacagg atcaaaaacg tcacggccgc gccgaagaac aggaaccaga
    10381 ccgccttggt gatagatata tctatgggtc ccagatgcaa ggggatctcc caggcgtcct
    10441 tgccgctctt gaaggtatgc aggatcttct cgcgcaactc ctcctgagtc acctccatga
    10501 gcgctagcat actcgtcacg cttctatcct ccgttctact gcctcacccg ctggccgccg
    10561 ctcgcccgga acacccatcg cccggacagc ccacggtacc agcaccaatg tctctacaaa
    10621 atacactgcc gtaaacccca tcagcatcgc taccaccggc cagagttcaa gataagccgg
    10681 gatacccagc gccaccgccg cgaagccgta tcgaaccccg aacgacgtgg ccccgagcaa
    10741 caccccgata accctggacc gccccgtcag gagcgagacc gttatagccg tggaggcgaa
    10801 actcacaata ccgacccccg caccatagca gaaagcgagc gaatggtccg gtccgtagac
    10861 gtaaaatatc gccccggccg ccgcagccgc cagaacgatc atccacgccg cgcacctgat
    10921 agcgacccgc cagtgaccgc tcatccgccg tccagcctct ggagcgcccg gtagacgtaa
    10981 tacagtccgc cgacgaaccc gagcgcgagc cctaacaaca ggaagagcgg gagcgtgcca
    11041 gcaaacttat ccacgaagaa accgactacc gtgggtaacg cgaagatcaa gatgagcgta
    11101 aaacccaaac cgataaaacg cccgtagcta ccgccggatc tgtttccttg agaatcggtg
    11161 tgaggcatcg cctgcactat accctaccgg ccaaatcgtg gcaatcagtt tcaagccgcc
    11221 agactccttc tggtatcatc cgcgactctg tcctcggcga cactatctca ccccccagcg
    11281 tttgcccgct gcgcgacgaa ggcttcgatg tgcccggcca acaactgtgg ctgatcttcc
    11341 gggacgaagg cgcgaatcgc tgactcgctt gagcaccgcg gcgggaaagt ctctccgcaa
    11401 ccgctcggcg tagcgcggcg ggaagaatat gtcgtcctcc ccccagacca gcaagaccgg
    11461 cttgtggaat tctcgaaaag atcgggcggc gtcgatcgta tagcggttgg agacgccctg
    11521 caggtagcgt gttagatctc gccgaacccc ggcatcggag atcaagggtc cgatataagc
    11581 atcgagcgtc cgcgtatcct ggcggcgctt cgagacggtc cagaaaagcc cgcgctgcgc
    11641 ggcgcgcgcc cgcagtagcc aggcgagagc gtccacgaac cgtaccccga agattcgggc
    11701 cgcgtattgg aagggtctga gcagcagcgg gaagaaggct tcgtaagcgt cgcagttggt
    11761 gaggacgagg cgcgagattt tatcactgtg ttcggctatc actatctggc agatggcccc
    11821 gcccgtgtca ttgccaatca gcgtgacatc ttcgagattc aacgtctcga tgagatcggc
    11881 gacgatcctg gcaactcctt tcggactgag atccgcatcc gcccccaagg ggatcgaatg
    11941 actgcccagc ggcagatcag ggacgatgca acggaactta tcagagagac gcgccactac
    12001 atgcctccag agcgttccat tcaccagtat gccgtgaacg aagaccagcg ccggcccact
    12061 ccccgcgtca cggtaacgga tcacaccctg ctccagccga acttccctca ttccaccgga
    12121 tactcgggtg accgccatgc cgctctccct tcacacgatt ttgtaatagc atgatactca
    12181 gttattgacg tgatgtcaat agacaccgtg tcaataaggt ggaagatcca ttagtattgg
    12241 ctacatgttg ggagaggtcg cagtgtgagc atgtcggaga ggcctcggcg gcggcatact
    12301 tcgcagcagg ccgagcgggc gatactggcg gcggcgaggt cttttctggc agagcatcca
    12361 ttccgggagt tgacggcgga ggcggtaatg acccgcacgg ggctgtccag gacggccttc
    12421 tacgcctact tccgggaccg ctacgatctg gttacgcggc tactggaggg tataggcgcg
    12481 ttgctcttcg cggtggaccg gtactggctg gagggcggcg aggaggctgg cgctcctgcg
    12541 agtgagagcc tgggagaagc actgaggcg
//
LOCUS       DASSMB010000298         6122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_736451, whole genome shotgun sequence.
ACCESSION   DASSMB010000298 DASSMB010000000
VERSION     DASSMB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736451"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VFJ72_16935"
     CDS             <1..654
                     /locus_tag="VFJ72_16935"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HET7481194.1"
                     /translation="TILENGARGVYPVESLEEADRVREKLDNDSLLRGGEQDAARIDG
                     YDHGPFPFEYAPETVAGKDVIFVTTNGTRAIADVAGADRVLLGNLRNAPAVARCLEAS
                     GTDSIYLVCAGSAGRFTIEDFLGAAAILSHMDTGGWTLNDAAWLALDFMERHAGKIEE
                     PLKQSRAGRWFVRNDRMDAFHFVADVGASGTVPEVVGGKLRRAEPEESPPLEVSDSN"
     gene            670..1728
                     /locus_tag="VFJ72_16940"
     CDS             670..1728
                     /locus_tag="VFJ72_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET7481195.1"
                     /translation="MPETISVREGEGFDLDVVERYLRKYIEGLPGGDLEVRQFPSGAS
                     NLTFLLKIGDWEGVLRRPPLGPVPPRAHDMGRESGILSKLNAAFPLAPKPYFFCGDES
                     VIGAPFYVMERRTGVAIDDSFPDDVEPTEELCRGISHTVVDTLVELHAVDPWRAGLGD
                     LGKPEGFLERQTHGWISRYDKAKTDEIEEVGPLTEWLAKDIPESPEPTIIHNDYKLNN
                     LLLDPGDLSEVRAVLDWEMATIGDPLFDLAVSLTYWVEPGDSEDLKQVLPTVTDTPGF
                     LTRREFIDRYAEKSGRDLSDMHWYVVFGYFKLAGILQQIYARWHNGQTKDERFANFDE
                     RVRALILHAHDLSRTGEV"
     gene            1728..2582
                     /locus_tag="VFJ72_16945"
     CDS             1728..2582
                     /locus_tag="VFJ72_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET7481196.1"
                     /translation="MPPNGKVGVLGTGTMGAGIVQLAAQSGYGVVACDFSVEALERAQ
                     VYVRQGLSRFASRGAFSEAEAAAHYDRIHWTTSVEDFRGAEVVIEAIVEKVGPKKEAF
                     AALDALIPPEACIFTNTSSISITDLASATERPEKVCGTHFFTPPPVREAVEIPRGLLT
                     SDETVERAKALVTSFGKLPIVVRKDVPGFVANRFLMPMIIEACRLLEEGVASKEDIDL
                     LVKKGLGFPIGPFELGDFIGLDVALDVTAYVHEELDESYYNPPRILKEMVRAGRLGHK
                     TGEGFYKY"
     gene            2635..3396
                     /gene="fabG"
                     /locus_tag="VFJ72_16950"
     CDS             2635..3396
                     /gene="fabG"
                     /locus_tag="VFJ72_16950"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HET7481197.1"
                     /translation="MGRLEGRVAMVTGAARGLGRATAERFAREGARVCLTDVDLEGAS
                     IAAKEMVDGGMDAFASRVDVTSRTEVENAVGRVVDRYGRLDILVNNAGVTRDNLLFRM
                     EDDEWRTVLNVHLTGAFLCSRAAQKHMVENSYGRIVNVSSTSALGNRGQANYSAAKAG
                     LQGFTKTLAIELGRYGVTVNSVAPGFIETEMTRATAERMGVDWETFVAGRVANIPVGR
                     GGRPEDVASAILYFASEEAGFVSGQVLYVAGGPRA"
     gene            3399..3851
                     /locus_tag="VFJ72_16955"
     CDS             3399..3851
                     /locus_tag="VFJ72_16955"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET7481198.1"
                     /translation="MLYEEFLGKRSEPVKNLIERGAVRKFAEAIADPNPLYVDEAAAR
                     AGRHGRLLAPPTFPRTFDYGRIVGLELPVAGMIHGEFRIFYERPLFVWDEVMCGMELK
                     DSYDKQSRRGLLGFLIFERTGESLDGDLIFTMDDIVVVTETVRKVILA"
     gene            3848..4276
                     /locus_tag="VFJ72_16960"
     CDS             3848..4276
                     /locus_tag="VFJ72_16960"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HET7481199.1"
                     /translation="MSGVPDGIPAWSVGEELEELELPPVNRLQLIKYAGASGDYNPIH
                     LTDEAAGEAGLPGIIAHGMLTVATMGLLFSPYLEHGYIKTFRSRFSGMVFLGDVLRVG
                     GRVTKTEDLEEGRLYACEVYARRNGDTVASGELGFLVYDG"
     gene            4283..4738
                     /locus_tag="VFJ72_16965"
     CDS             4283..4738
                     /locus_tag="VFJ72_16965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7481200.1"
                     /translation="MGEKMETEGTLDREQLTRLAQYFDEKVTFSKHIKSRVEEVEKGR
                     AVCYIDVEDIHLNGNGTLHGGVYASLIDNAMGLSVAALVGLRTATIALNVSFLGAVRE
                     GRIVCESRVVHRTRRIATVEAKVHDAEENLVAIGTGTFRIFEKRGDPIV"
     gene            4860..>6122
                     /locus_tag="VFJ72_16970"
     CDS             4860..>6122
                     /locus_tag="VFJ72_16970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7481201.1"
                     /translation="MFSTDRPWLKAYEGYAEPETEIFPGSIYDFFRDSAERHGDKTAL
                     TFYGTQFSFQRLLALSEKMAASLAASGVKKGDRVALMLPNCPQYVVSFFGVLKLGAIV
                     TQLNPMYVEREIEHILRDSGAETIAIYSDMYERVKSVLPASSVRNVVVVDFRDEPEGL
                     DAGHHSFGDFLGMDADPAPQAEIEPAEDVAALQYTGGTTGVSKGAMLTHRNLVANVQQ
                     ALDLFIDDPGAFSNNQAVMGILPMFHIFGLTCVMLFAIRQGLNQLLLPRFDPGEVLKL
                     VREHRPVMFSGVPTMFMALNSSGADLRKHRFHEVRVYNSGGAALPVNLKRSFEDKTGR
                     PLFEGYGLSEASPVTHFNPPFTGAGREGSIGVPVPSTDARIVDVESGEREMPVGEPGE
                     LIIKGPQVMKGYRNMPEETARTLRDGWLY"
BASE COUNT         1114 a         1934 c         2115 g          959 t
ORIGIN      
        1 accatcctgg agaacggcgc tcgcggcgtc tatccggtcg agagcctgga agaggccgac
       61 cgcgtgaggg agaagctgga caacgactct ctactgcgcg gcggagagca ggacgccgcc
      121 cgcatagacg gctacgacca cggcccgttc cctttcgagt acgcgccgga gaccgtcgcc
      181 gggaaggacg tcatcttcgt gaccacgaac gggacccgcg ccatcgccga cgttgccggc
      241 gccgaccggg ttctcttagg caacctccgc aacgccccgg ccgtcgcccg ctgcctcgaa
      301 gcctcgggaa cggactctat ctacctggtg tgcgccgggt ccgccgggcg cttcacgatc
      361 gaggacttcc tgggagcagc cgccatactc tcccacatgg acaccggcgg ctggacgctg
      421 aacgacgcgg catggctcgc cctggacttc atggagcgcc acgcggggaa gatagaggag
      481 ccgctcaagc agagccgggc cggacgctgg ttcgtgagga acgaccggat ggacgccttc
      541 cacttcgtcg cggacgtcgg ggcgagcggg accgtcccgg aggtcgtggg cgggaagctt
      601 cgccgcgcgg agccggagga gagcccgccg ctggaggtct cggattcaaa ctagagaagg
      661 acggcgaaca tgccagagac gataagcgtc cgtgaaggag aaggcttcga cctcgatgtc
      721 gtcgagcggt acctgcggaa gtatatagag ggcttgcccg gcggggatct ggaggtgcgg
      781 cagttcccgt ccggcgcttc caacctgacg ttcttgctca agatcgggga ctgggagggc
      841 gtgctccggc gtccgccgct cggtccggtg ccaccgaggg cgcacgacat gggccgcgag
      901 tccggcatcc tctcgaagct caacgccgcc ttcccgctcg ccccgaagcc ctacttcttc
      961 tgcggggacg agtcggtcat cggggcgccg ttctacgtca tggagcggag gacgggcgtc
     1021 gccatagacg actcgttccc ggacgatgtc gagccgacgg aggagctgtg ccgcgggatc
     1081 tcgcacaccg tcgtggacac gctggtggag cttcacgcgg tcgatccgtg gagggccggg
     1141 ctcggggacc tcggcaagcc ggaaggtttc ctggagcggc agacgcacgg ttggatctcg
     1201 cgctacgaca aggccaagac cgacgagata gaggaggtcg ggccgctcac cgaatggctc
     1261 gcgaaggaca tccccgagag ccctgagccg accatcatcc acaacgacta caagctcaac
     1321 aacctcctcc tcgaccctgg cgacctgagc gaggtccgcg ccgtgctcga ctgggagatg
     1381 gcgaccatcg gagacccgct cttcgatctg gccgtctccc tgacgtactg ggtcgagccg
     1441 ggcgactccg aggacctcaa gcaggtcctg ccgaccgtga cggacacccc cggcttcctc
     1501 acgcgcaggg agttcataga ccgctacgcc gagaagagcg ggcgcgacct ctcggacatg
     1561 cactggtacg tcgtcttcgg ctacttcaag ctcgcgggga tactgcagca gatctacgcc
     1621 cgctggcaca acggccagac aaaggacgag cgcttcgcca acttcgacga acgggtgcgc
     1681 gccctgatcc tccacgccca cgacctctcg cggaccggag aggtctgatg cccccgaacg
     1741 gaaaggtagg cgtcctcggc accggcacga tgggcgcggg catcgtccag ctcgccgccc
     1801 agtccggcta cggagtcgtg gcctgcgatt ttagcgtcga ggcgctcgaa cgggcccagg
     1861 tctacgtccg ccagggcctc tcccgattcg ccagccgcgg cgcgttctcc gaggccgaag
     1921 ccgccgccca ctacgaccgc atccactgga cgacgagcgt ggaggacttc cgcggcgcgg
     1981 aggtcgtaat agaggcgata gtcgagaagg tcggccccaa gaaggaagcc ttcgccgcac
     2041 tagacgccct gataccgccc gaggcgtgca tcttcaccaa cacctcctcc atctccataa
     2101 cggacctcgc ctccgccaca gagcggcccg agaaggtctg cggcacgcac ttcttcaccc
     2161 cgccgccggt gcgcgaggcg gtggagatac cgcgcggcct gctcactagc gacgagacgg
     2221 tcgagcgggc gaaagccctc gtaacctcct tcggaaagct gcctatcgtg gtccggaaag
     2281 acgtgccggg gttcgtggcg aaccgtttcc tgatgcccat gatcatcgag gcctgccgcc
     2341 tcctcgaaga gggcgtggcg agcaaggaag acatagacct cctcgtaaag aagggtctcg
     2401 gcttccctat aggcccgttc gagctcggcg acttcatcgg tctcgacgtg gctctcgacg
     2461 tgacggccta cgtccacgag gagctggacg agtcctatta caacccgccg cgcatcctga
     2521 aggagatggt ccgcgccggc aggctcggcc acaagaccgg cgaagggttc tacaaatact
     2581 agaagcacta ggagcgcggt ttctctggcg cggcggctga ccggaaggag gcacatggga
     2641 agactcgaag gcagggtggc gatggtgacc ggcgcggcgc ggggtctcgg gcgggcgacg
     2701 gcggagcggt tcgccaggga gggcgcgcgg gtgtgcctga cggacgtgga cctcgaaggg
     2761 gcctccatcg cggcgaagga gatggtggac ggcggcatgg acgccttcgc gagccgggtg
     2821 gacgtcacca gccgcacgga ggtcgagaac gcggtcggca gggtggtgga tcgctacggg
     2881 cggctggata tcctggtgaa caacgcgggc gttacgcggg acaacctcct tttcaggatg
     2941 gaggacgacg agtggcgcac cgtactcaac gtacacctga ccggcgcgtt cctgtgcagc
     3001 cgcgccgccc agaagcacat ggtcgagaac agctatggcc gcatcgtcaa cgtctcctcc
     3061 acctcggccc tcggcaaccg gggccaggcg aactactcgg cggcgaaggc cggtctccag
     3121 ggcttcacca agacgctcgc catcgagctc gggcgctacg gcgtcaccgt caactccgtc
     3181 gctcccggct ttatagagac ggagatgacg cgggcgacgg cggagcggat gggcgtggac
     3241 tgggagacgt ttgttgcggg ccgggtggct aacatacccg tagggcgcgg tggccggccg
     3301 gaggacgtgg cctccgccat cctgtacttc gcctcggagg aggccgggtt cgtgagcggg
     3361 caggtactct acgtcgccgg cggaccgagg gcgtagcgat gctctacgaa gagttcctcg
     3421 gtaaacgctc cgagccggtc aagaacctca tcgagcgtgg ggccgttcgc aagttcgccg
     3481 aggccatcgc cgacccgaac ccgctctacg tggacgaggc cgcagcgaga gctggccgcc
     3541 acgggcgtct gctcgccccg ccgaccttcc cgcgcacctt cgactacggg cggatcgtgg
     3601 ggctggagtt gccggtggcg ggcatgatcc acggcgagtt ccgcatcttt tacgagcggc
     3661 cgctctttgt ctgggacgag gtgatgtgcg gcatggagct aaaggactcc tacgacaaac
     3721 agagccggcg ggggctgctg gggttcctga tcttcgagcg gaccggcgag agtctggacg
     3781 gagatctcat cttcaccatg gacgacatcg tcgtcgtcac ggagacggtg aggaaggtga
     3841 tcttggcgtg agcggtgtgc cggacggtat cccagcctgg agcgtgggag aggagctgga
     3901 ggagctggaa ctgccgcccg tgaaccggct gcagctcatc aagtacgcgg gggcatcggg
     3961 cgactacaac ccgatccacc tgaccgacga ggccgccggg gaggccggtc tccccggcat
     4021 catcgcccac gggatgctga ccgtcgccac gatggggctg ctgttctccc cctacctgga
     4081 gcacggttac atcaagacct tccgcagccg cttctccggt atggtcttcc tgggcgacgt
     4141 gctgcgcgtg ggtgggaggg tcacaaagac cgaggatctg gaagagggac gcctgtacgc
     4201 ctgcgaggtg tacgcccggc ggaacggaga cacggtggcg tccggcgaac tcgggttcct
     4261 ggtctacgac ggctaattcg agatgggaga gaagatggag acagagggca cactggacag
     4321 ggagcagttg acccgcctgg cgcagtactt cgacgagaag gtcacgttct cgaagcacat
     4381 aaagtcccgc gtggaggagg tcgagaaggg acgggcggtc tgctacatag acgtggagga
     4441 cattcacctc aacggcaacg ggacgctgca cggcggcgtc tacgcctcgc tcatagacaa
     4501 cgctatgggg ctctcggtgg cggcgctggt ggggctcagg acggcgacca tcgcgctcaa
     4561 cgtcagcttc ctcggggcgg tgcgggaggg acgcatcgtg tgcgagagcc gggtggttca
     4621 ccggacgcgc aggatcgcca cggtcgaggc gaaggtccac gacgcggagg agaacctggt
     4681 cgccatcggg acggggacgt tcaggatctt cgagaagcgc ggcgacccga tagtgtaggc
     4741 cctgtagtct ctgtaggccc gcgcgggtcc gcggcgtttg acccgtcccc gttccggtaa
     4801 gataggctca gaacggggga cacgcgccgg agcgctccgg cttcatccaa ggaggacgca
     4861 tgttcagcac cgacaggccg tggctgaagg catacgaggg atacgcggag ccggagacgg
     4921 agatattccc cggttcgatc tacgacttct tccgcgactc ggccgagcgg cacggcgaca
     4981 agaccgccct gacgttctac ggtactcagt tctctttcca gcggctactg gccctctccg
     5041 agaagatggc cgcctccctc gccgccagcg gcgtaaagaa gggagaccgg gtcgcgctga
     5101 tgctccctaa ctgtccgcag tacgtcgtct ccttcttcgg ggttctcaag ctcggcgcta
     5161 tcgtcacgca gctcaacccg atgtacgtcg agcgcgagat agagcacatc ctgcgcgact
     5221 ccggcgcgga gactatagcc atctactcgg acatgtacga gcgcgtgaag tccgtcctcc
     5281 cggcgtcgag cgtgagaaac gtcgtcgtgg tggacttcag ggacgagccg gagggcctgg
     5341 acgccggcca tcacagcttc ggagacttcc tgggtatgga cgccgacccc gcgccgcagg
     5401 cggagatcga gccagccgag gacgtggccg ccttgcagta cacgggaggc acgaccggcg
     5461 tctcgaaggg cgcgatgctc acgcaccgca acctggtggc gaacgtgcag caggcgctcg
     5521 acctgttcat agacgacccc ggagctttct ccaacaacca ggcggtgatg ggcatcctac
     5581 cgatgttcca catcttcggt ctgacgtgcg tgatgctctt cgctatccgg caggggctga
     5641 accagctact cctgccgcgc ttcgaccccg gcgaggtcct gaaattggtc agggagcacc
     5701 ggcctgtgat gttcagcggc gtgccgacga tgttcatggc gctcaacagc agcggcgcgg
     5761 acctgaggaa gcaccgcttc catgaggtca gggtctacaa ctccggcggc gcggccctgc
     5821 cggtgaacct caagcgctcc ttcgaggaca agaccggacg gccgctcttt gaaggctacg
     5881 ggctctcgga agcctcgccg gtgacgcact tcaacccgcc gttcacgggc gcggggcgcg
     5941 aggggagcat cggggtcccc gtgccgagca ccgacgcgag gatcgtggac gtggagagcg
     6001 gggagcggga gatgccggtc ggagagccgg gggagttgat cataaaggga ccgcaggtga
     6061 tgaagggcta ccggaacatg cccgaggaga cggccaggac tctcagggac ggctggctct
     6121 ac
//
LOCUS       DASSMB010000299         6007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_744222, whole genome shotgun sequence.
ACCESSION   DASSMB010000299 DASSMB010000000
VERSION     DASSMB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744222"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..1268)
                     /locus_tag="VFJ72_16975"
     CDS             complement(252..1268)
                     /locus_tag="VFJ72_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7481202.1"
                     /translation="MIAPLALITGGLLVGLIFEKLVMKKLKAVVSGTKFRGDDIVVDS
                     VKGITTLWFGISGVYLAVVKLPLDGAISDLLKKVLMVALISSAALVLARIAAGLIKLY
                     TGRVQGLPSSSLLTNLARMAILIVGALIILQSLGVAIAPVMAALGVGGLAVALALQET
                     LSNLFCGILIIVSRQLRPGDFVRLDSGDEGYVTDVTWRNTTIRSLSNNMIIVPNARLA
                     QSTVTNFYQPERRMSVVVPVGVSYDSDLSNVERVTVEVANHVMEEIEGGVPEFEPSVR
                     FNNFGDFSIDFSVSMQTREVVEQYRIKHEFIKRLHERYWKEGIEIPSPPVATVPVSGK
                     SQEA"
     gene            complement(1453..1878)
                     /locus_tag="VFJ72_16980"
     CDS             complement(1453..1878)
                     /locus_tag="VFJ72_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7481203.1"
                     /translation="MPEMTSEEYRGFMLDRARTASLATVRADGRPHVAPIWFDLDGDT
                     LVFMTGEDTVKGRNMRRDPRVSLCVDDEEPPFHFVFFEGVAELAPDDPDKLYWATRIA
                     GRYMGEGLAESYGRRNAVPGELLVRVRPSRVVAQKNIAD"
     gene            complement(1908..2447)
                     /locus_tag="VFJ72_16985"
     CDS             complement(1908..2447)
                     /locus_tag="VFJ72_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HET7481204.1"
                     /translation="MARDTEEAITVLGKAVQDAMNEQIKNELFSAYQYLSMAAYCESA
                     NLPGFAAWMRAQAREEMEHAMKFYDFILDRNGRVVLRAIEEPVVEFGSPLEVFERALE
                     HERKVTAMINDLYGLSVKENDYASQTFLQWFVTEQVEEEKNAGDVVETLKMVGDRSEA
                     LFLLDRELGQRRAGEQPAD"
     gene            2654..3217
                     /locus_tag="VFJ72_16990"
     CDS             2654..3217
                     /locus_tag="VFJ72_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481205.1"
                     /translation="MDERRIFLTLDGYYEHDEEKRSVQHQDERALRWRENLARARRRE
                     RARERGWARLAAAWVAAVRWEDLARKREQDEKRKRAEAEAAWEEFTRTERHELKLRQD
                     GQLARLLGERLPRELPATLQRLADEDRRQALAGMVALMSNGSTKYKNISDLCPADMPA
                     RIAANRLRTAWLKERRDQWLGRWEPPL"
     gene            complement(3610..3819)
                     /locus_tag="VFJ72_16995"
     CDS             complement(3610..3819)
                     /locus_tag="VFJ72_16995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481206.1"
                     /translation="MMPAEATAEVFWTAFTALPAETREKVIEKMLADSVLREEVEDLL
                     DLEVVRERSSEPARPLDDVLAELND"
     gene            complement(4077..4445)
                     /locus_tag="VFJ72_17000"
     CDS             complement(4077..4445)
                     /locus_tag="VFJ72_17000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET7481207.1"
                     /translation="MTRGKVVLVPFPFDDLSTTKVRPAVCLTDPVGVHRHIILAFITS
                     RVPDELLETDIVLDPARAEHAATGLRVRSVLRLHRMMTVSTSLLQRELGELSTDAQAE
                     VDGKIRYLFALEDPASGSSP"
     gene            complement(4442..4759)
                     /locus_tag="VFJ72_17005"
     CDS             complement(4442..4759)
                     /locus_tag="VFJ72_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481208.1"
                     /translation="MIDSCYAEQASYRPRRKQTGELVDGTLRAIEVEGTVDEQHRLHV
                     DEPLPVDGPGRVRVLVLIPDASGPEDPEWLRAAASNPAFDFLQHEDEDLYGPADGRPF
                     LDH"
     gene            complement(5024..5197)
                     /locus_tag="VFJ72_17010"
     CDS             complement(5024..5197)
                     /locus_tag="VFJ72_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7481209.1"
                     /translation="MAARYALEIKRSAQKSISRLPKRDAERVRDAIDALAEDPAPRGA
                     GTVVGTPFLRIRV"
     gene            complement(5201..5467)
                     /locus_tag="VFJ72_17015"
     CDS             complement(5201..5467)
                     /locus_tag="VFJ72_17015"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET7481210.1"
                     /translation="MQRSITASEARDEFADVINRVAYGGERVVIRRREKELAAVIPME
                     DLRLLERLIEQEEDRLDAEAARKALADSDDEAVPWEEARKELGL"
     gene            complement(5725..>6007)
                     /locus_tag="VFJ72_17020"
     CDS             complement(5725..>6007)
                     /locus_tag="VFJ72_17020"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HET7481211.1"
                     /translation="PQEGQAGVKFDYYPDTDSLYIELNPGRERKPGGTQEVVGWGDHD
                     IVVDVDGDGVPVGIDIDSFASRIVDITKLEAEGPIFGLVRSPDSERRVS"
BASE COUNT         1130 a         1990 c         1743 g         1144 t
ORIGIN      
        1 gtagacctcg cgccaggtat cgagcggttg ggccacggcc tcgcgcaacc gctccgcgac
       61 ctcatccggg gccgagccta tctccgcccc gcttccgtta gagtcaacat tcttcatgca
      121 aaagataata acccacggcc gcggaaggtg aagcctggcc gtcagcctgg catcagccgc
      181 atgaccgact gcggtaccga ccacggtacc ggccacgtat ctgcgggcag cagtcttgag
      241 ccgggtctac gttacgcttc ttggctcttg ccgctgacgg ggacagtggc gacgggaggc
      301 gacggtatct cgatcccttc tttccagtag cgttcgtgga ggcgcttgat gaactcgtgc
      361 ttgatgcggt actgttccac gacctcccgc gtctgcatgc tgacgctgaa gtcaatgctg
      421 aagtcaccga agttgttgaa ccggaccgaa ggctcgaact cggggacgcc gccttctatc
      481 tcctccataa catggttagc gacctcgacg gtcacccgct ccacgttgga gagatctgaa
      541 tcgtagctga cgcccaccgg cacgaccacc gacatccggc gctcgggctg gtagaagttg
      601 gtgacggtag actgcgccag ccgggcgttc ggcacgatga tcatgttgtt cgagagcgag
      661 cggatcgtcg tgttccgcca ggtaacatcc gtgacgtaac cctcgtcgcc cgagtcgagc
      721 ctcacgaagt cccccggacg aagctgccgg gagacgatga tcaggatgcc gcagaacagg
      781 ttggagagcg tctcctgaag cgccagggcg accgccagcc cgccgacccc cagagccgcc
      841 atcacgggcg ctatggcgac cccgagagac tggaggatta tgagggcgcc gacgatgagg
      901 atcgccatcc gcgccaggtt ggtaaggagc gatgacgagg gcaagccctg cacccgtccc
      961 gtgtacagct tgatgagacc ggcggctatc cgcgccagga ccagcgcggc ggatgagatc
     1021 agggccacca tcaagacctt cttgagcagg tccgagatcg caccgtccag cggaagcttc
     1081 acgaccgcca ggtagacacc ggagatcccg aaccacaggg tcgttatccc cttgacggag
     1141 tcaaccacga tgtcgtcgcc gcggaacttc gtcccggaga cgaccgcctt cagcttcttc
     1201 atgaccagct tctcgaagat cagcccgacg agcagccccc cggttatcag ggcgagaggc
     1261 gcgatcaact ctttcgtctc gatgagcgcc agacccggta gctccccggc atgaacagct
     1321 aggtaactga tagttctccc ctccgtgacc tcgcttgcgc actccctgca aacggcgcac
     1381 accctaacgc agacgagcgg cgacctcaat ccccacagcc gggcggccag tgttccagat
     1441 cacaggccgc cgctaatcgg cgatgttctt ctgggcaacg accctggacg gcctgaccct
     1501 cacgagcagc tcccccggca cggcgttccg gcggccgtag gactcggcga gaccctctcc
     1561 catgtagcgc ccggcgatgc gcgtggccca gtagagcttg tccgggtcgt cgggagcaag
     1621 ctcggccacg ccctcgaaga agacgaagtg aaacggcggc tcctcgtcgt caacacacag
     1681 gctcacccgc gggtcccgcc gcatgttccg gccctttacc gtatcctcgc ccgtcataaa
     1741 gacgagagtg tcaccgtcga ggtcgaacca gatcggggcg acgtgcggac gcccgtcggc
     1801 ccgcaccgtg gcgaggctgg ctgtgcgcgc ccggtcgagc atgaagcccc ggtactcttc
     1861 actggtcatc tccggcatcg cgtcagcctt cctgtcgggt cttcacatca gtcggcgggt
     1921 tgctcgcccg cgcggcgctg gccgagttcc ctgtcgagca ggaagagcgc ctcgctcctg
     1981 tcgccgacca tcttcagcgt ctccacgacg tccccggcgt tcttctcctc ctcgacctgc
     2041 tccgtcacga accactgcag aaatgtctgg ctcgcgtaat cgttctcctt gaccgacaga
     2101 ccgtagaggt cattgatcat ggccgtcacc ttccgctcgt gttccagcgc ccgctcgaag
     2161 acctccagcg gagagccgaa ctccacgacc ggctcttcta tcgcccgcag gacgacccgt
     2221 ccgttgcggt cgaggatgaa gtcgtagaac ttcatggcgt gctccatctc ctcccgcgcc
     2281 tgcgcccgca tccaggccgc gaagcccgga aggttcgccg actcgcagta cgccgccatc
     2341 gagaggtact ggtaggccga gaagagctcg ttcttgatct gctcgttcat cgcatcctgg
     2401 actgctttac ccagcacggt aatcgcctcc tcggtgtctc tcgccacgtc ccagcctcca
     2461 ctatagcgct ctcttcaccc ataagcccgt acaactcgcg gacgaagcag tcccgttcca
     2521 gatcaacctg accggattat gatacaataa gcattaatag gaaagtcggc tagctctctg
     2581 gagacggagc cgccttttcc gccccgccgg tatgcaaact cgggcgcgct cctctttcga
     2641 gattcgcgag cttgtggatg agagaaggat atttctgacg ttggacggct actacgagca
     2701 tgatgaagag aagcgctccg tgcagcatca ggacgagagg gcgctacggt ggcgggagaa
     2761 ccttgcgcgg gcgcgtagac gagagcgggc cagggagcga ggatgggcga ggctcgccgc
     2821 cgcctgggtc gccgcggtca ggtgggagga ccttgcgcgg aagcgcgagc aggacgaaaa
     2881 gagaaagcgg gcggaagcgg aggccgcctg ggaggagttc actcggacgg agagacatga
     2941 gctcaaactg cgccaggacg gccagctcgc caggcttttg ggcgaacggc ttccgagaga
     3001 gctaccggcg acattgcaga gactcgctga tgaggaccgt agacaggccc tggcggggat
     3061 ggtagcgcta atgagcaacg ggagcacgaa gtacaagaac ataagcgacc tttgcccggc
     3121 ggacatgccg gccaggatcg cggcgaacag gttgcgaaca gcgtggctca aggagcggag
     3181 ggatcagtgg ctcggtcggt gggagcctcc gctgtagaaa ctccgcgcgg ctaccgcgcc
     3241 tcctctcctc ctcggctaca gcgatctact cgtcggtgag gccgtacagc tcgtagacga
     3301 ggcggtctat ctgcttatcg gcggcgtagt aggtgcggct agatcttccg gtaaacctcg
     3361 cggcgatgcc caatggcacg aatcataacg atgcgagctt cgttatctac cgagtagatg
     3421 acccggtagt cgcctactcg aagtctaaag tgaggactac ccttcaaccg ctttgcttgc
     3481 gccggatatt gatcttcgcc aagcgcccgc atacgctcac ctatccgccg cgcaacttca
     3541 ggggctagag atttaagctc gcgctcggcc gagcggacga ttacgacatc gtagggcaac
     3601 agacaagggt tagtcgttta gctccgcgag tacatcgtcc aacggacggg cgggttcgct
     3661 ggagcgttct ctcacgactt ccaggtccag caggtcctcc acctcttcgc gcaggacact
     3721 atcggcgagc atcttctcga tgaccttctc gcgcgtctcc gcgggcaacg cggtgaaagc
     3781 cgtccagaaa acctcggcgg tcgcctctgc tggcatcatg attgcgctcc ttcttcctct
     3841 tgcaaacccg gcttcggaat ggccggccgc cggtccaagt ccagaatcgc aggcagctcg
     3901 ccaccaccat ctgctcacca gagtctatca ccgggccgtc gcctcctcga ctacccgtgt
     3961 ctcctcgtcg gtgagaccgt atagctcgta gacgagacgg tctatctggc ggtcggtggc
     4021 ggagaacggg tgcccgatca cgctcgatct tcgcccccgt caaccgctcc cgcggcttac
     4081 gggctcgacc cgctcgcagg atcttcgagc gcgaataggt atcgtatctt gccgtcgacc
     4141 tcggcctgcg cgtccgtcga aagctcaccc agttcgcgct ggagcaggga tgtcgaaact
     4201 gtcatcatcc ggtgcaaacg gagcacggat ctgacccgca gaccggttgc cgcatgttcg
     4261 gctcgcgcgg ggtccaggac gatgtcggtc tccaacaact cgtctggtac gcggctggtg
     4321 atgaacgcca ggatgatatg ccggtggact ccgacggggt ccgtgagaca tacggccgga
     4381 cgtaccttcg tcgtcgatag gtcgtcgaag gggaatggaa cgagtacgac tttacccctg
     4441 gtcaatggtc aagaaaaggc cgtccatcgg ccggaccgta aaggtcctca tcctcgtgct
     4501 ggagaaagtc gaatgccggg ttagaggccg cggcccgcaa ccattccgga tcctccggcc
     4561 ccgaagcgtc ggggatcaag accagcactc gcacacggcc cggaccatcc accggcaggg
     4621 gttcgtccac atgcagccgg tgctgttcat ccaccgtacc ctctacctcg atagctctca
     4681 aggtgccgtc cacaagctct ccagtctgct ttcttcgggg cctgtacgaa gcctgctctg
     4741 cgtaacagga gtctatcacc gggccgcagc ctcctcgact acggcggtct cctcatccgt
     4801 gaggcctgac aactcataga cgaggcggtc tatctggcgg tcggtagcgt agaacgggtg
     4861 cccgatcacc accacccact cccgctcgat ctccgccccg gccggccact cacagagcgc
     4921 tatcgccgct agaggctccg gtacacctcc cgcctatgtg tcctacccgg attaccagta
     4981 ccaggagctg ctcctgccgt acctcgtata cgattcggta acttcagacc cggattcgca
     5041 agaagggggt acccaccacg gtcccagccc cgcgcggcgc aggatcttcg gccaaagcat
     5101 ctatggcgtc acgtacccgc tcggcgtctc tcttcggcag gcgcgagatg gacttctgcg
     5161 cgctgcgttt gatctcgagc gcgtagcggg ccgccacctc ctatagcccg agctccttcc
     5221 tggcctcttc ccaggggacg gcttcgtcgt ccgaatcggc cagagccttc cgcgccgcct
     5281 cagcgtccag ccgatcctcc tcctgctcga tcagacgctc aagaaggcgc agatcctcca
     5341 tcgggatgac cgccgcaagc tctttctcgc gccgacgaat taccacccgc tctcccccat
     5401 aagccacccg attgataacg tcggcaaact cgtcccgagc ttcgctcgca gtgatgctcc
     5461 gctgcatttc gactcctacc ttacgttttg tacgaaacgt agtctaactg attttcgtac
     5521 tcgcgcattt cggaaacctg cggcctgcgc cctacggtac gctagctgga gtcgttaccg
     5581 aaccgaagct tcctcgacta cctggatctc ttcatcggtg aggccgtaca gctcatagaa
     5641 gaggcgatcc atctgccagt cggtggctgc tatttgttgc ccgatcaccg tccgctcgcg
     5701 tagcgtttcc cggaccagtt ccgtctaact cactcgtctt tcagagtccg gcgagcggac
     5761 aaggccgaag attgggcctt cagcctcaag cttggtgatg tcaacgatcc tggacgcgaa
     5821 gctgtctata tctataccca ccggcacccc gtcgccgtcc acatctacga cgatatcgtg
     5881 gtctccccag cccactactt cctgcgtgcc gccgggcttg cgttctcttc caggattcaa
     5941 ctcgatgtac agtgaatccg tatctggata gtagtcgaac ttcactccgg cctgcccttc
     6001 ttgcggg
//
LOCUS       DASSMB010000300         4904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Rubrobacteraceae bacterium isolate SMAG_U1187
            k141_1879316, whole genome shotgun sequence.
ACCESSION   DASSMB010000300 DASSMB010000000
VERSION     DASSMB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963343
            Sequence Read Archive: SRR25158187
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rubrobacteraceae bacterium (soil metagenome)
  ORGANISM  Rubrobacteraceae bacterium
            Bacteria; Actinomycetota; Rubrobacteria; Rubrobacterales;
            Rubrobacteraceae.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 04:35:09
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,408
            CDSs (total)                      :: 3,360
            Genes (coding)                    :: 3,344
            CDSs (with protein)               :: 3,344
            Genes (RNA)                       :: 48
            tRNAs                             :: 45
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 16
            CDSs (without protein)            :: 16
            Pseudo Genes (ambiguous residues) :: 0 of 16
            Pseudo Genes (frameshifted)       :: 2 of 16
            Pseudo Genes (incomplete)         :: 14 of 16
            Pseudo Genes (internal stop)      :: 3 of 16
            Pseudo Genes (multiple problems)  :: 3 of 16
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Rubrobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1879316"
                     /isolate="SMAG_U1187"
                     /isolation_source="soil"
                     /db_xref="taxon:2740537"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.08083333 N 115.01 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFJ72_17025"
     CDS             <1..896
                     /locus_tag="VFJ72_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HET7481212.1"
                     /translation="SGTTALWEYLNEHPQVYLCTPKHTRFFASEGKVPDFGGPKPPGL
                     GAKSTRRAPYAITDIEEYRRLFDGAAGRAAIGEASHSYLYTPGTPERVRYYAPDAKLI
                     AILRDPVKRAYSHFCFMVQNGREPLDDFARAVEEEEAGRRDDWWPTFQYVRVGFYYAQ
                     LRPYFDLFERDQIRVYLQEDLHSDPLSVVQDVFRFLNIDDSYVPDVTKKHNGSGIPKN
                     KMLETLLGRPNPLKSILKETLPEKQRAHISARFARMRSRNLARPPQLPAELRARLVEK
                     YREDILNLQGLIQRDLSSWLV"
     gene            1059..2351
                     /locus_tag="VFJ72_17030"
     CDS             1059..2351
                     /locus_tag="VFJ72_17030"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase"
                     /protein_id="HET7481213.1"
                     /translation="MFERCAVVFVFLLASTGLLLPLLHEKGGAAAQSAAQGDPVTQVV
                     WFGVYAVMFLMFLVQWRRFLGIALKDRLLLLLTVVALASVLWAVSPEVTERRSVALIG
                     STLFGIYLASRFSLSEMLRLLAWAMGIAVVLSLLFVLALPSYGISGASLTQGDWQGIF
                     GQKNTLGKNMALSITVFLILAMSSRSHRWIALSGIGLSSVLLLFSHSTTSLVVAVSII
                     CLVPFFRGLRWKRTLAVSLVILTILLSGSFAIWFVSNPQIVLGALGKDTTLTGRTVLW
                     SAVLKAIHERPWLGYGYSSFWLGWEYPSADVWLATRGNYYDSHNGILDLWLQLGFLGI
                     CIFALNFLRALLRAVAWVRATVTAEGIWPLAFLSFMLLSNLTEGTILQQNNLSWILYV
                     ATVFSIATPPRNPAGRSNNHSGVGEYPEPLERRRALIR"
     gene            2671..3585
                     /locus_tag="VFJ72_17035"
     CDS             2671..3585
                     /locus_tag="VFJ72_17035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7481214.1"
                     /translation="MCVITYRRVGGLRRLLESLEGLIFNKAELPEMEIVVVDNDPAGS
                     ARAVCEELGSDSRWLLRYHIEPRRGISHARNAAVAFAEAPDFIAFIDDDEVPKPYWLD
                     ELLDVQKAYAADVVSGPVLTYFCDPVPNWVVRGRFFEQAFANPRYVTGHPIQLTATGN
                     VLIRAEIFGDASFDERLGLTGGEDINFFGRVHQAGYKMVYAGDALVYEWVPGSRTNVR
                     WILKRAYRLGNALSLSEACLDPTPAVRLVRVVKGGGRIAQGLLVLPLFVPLSLALGRH
                     LSVKPLLHICRGTGMLAGLAGIRFEPYR"
     gene            3678..4889
                     /locus_tag="VFJ72_17040"
     CDS             3678..4889
                     /locus_tag="VFJ72_17040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7481215.1"
                     /translation="MDRPPSSLLFIHASADLYGSDITLLQIVSGLDRDRFRAVVVVPY
                     AGPLVPRLQEAGAEVLVYPGLPVVRRQCLRLTGLLRLTLSSLKTALWLAGVMRRRNVA
                     LVHNSTLAVALAGLAAGLARRPQLWHVQEIVTRPRVVASLLATISSVVSTVVVANSQA
                     TAGHYHRTRFTASAPVRVIPNGVDESRLRDRAGTASRSLVEASPEDTVFTLVGRINHL
                     KGHAVFLDAAERIAAESENVRFLIVGDSFAGQEELTEAVDERIESSDLLRGRAIRLPH
                     TAEIGNVYEASDVVVVPSTEPESFGLVAAEAMAAGLPVIASRIGALPEVVNDGSTGIL
                     VDPGDATSLLAAMRKLHLSPSGRVRMGRLGRERFERCFRVETYVEGFNRVYEELLRKE
                     RDYVFSNAGRG"
BASE COUNT          875 a         1499 c         1541 g          989 t
ORIGIN      
        1 gatccggtac cacggcgctc tgggaatacc tgaacgagca tccgcaggtt tacctgtgta
       61 ctcccaagca cacgaggttt ttcgcctccg agggcaaggt ccccgacttc ggcggaccga
      121 aaccccccgg tctgggcgcc aaaagcacga gaagggcacc atacgccatc accgacatcg
      181 aggagtatcg gcgcttgttc gacggagccg ccggtagggc tgcgataggc gaggcgtccc
      241 actcgtacct ctacacgcca gggacccccg agcgcgtgcg ctactacgcg cccgatgcaa
      301 agctcatcgc gatactgcgc gatccggtca agagggctta ctctcatttt tgctttatgg
      361 tccagaacgg ccgcgagccc ctcgacgact tcgcgcgcgc ggtcgaggag gaagaggctg
      421 gtaggcgcga cgactggtgg ccgacctttc agtacgtgcg ggtgggtttc tactacgctc
      481 agttgcggcc ctacttcgac ctgttcgagc gcgaccagat ccgggtctat ctccaggaag
      541 atttacactc cgatcccttg agcgtggtgc aggacgtctt ccgcttcctg aacatagacg
      601 attcttacgt cccggacgtg acgaagaagc acaatggatc gggcatcccg aagaacaaga
      661 tgctcgaaac cctgctggga agacccaacc cgctcaagag catactcaag gagactcttc
      721 cagagaagca gcgcgcccac atctccgcca ggttcgccag gatgaggtcc agaaacctcg
      781 ccagacctcc gcaactaccg gcagagttga gggcgaggct ggtcgagaag taccgggaag
      841 acatcctgaa ccttcaaggt ctcatccagc gagacctttc gtcctggctc gtctgatgga
      901 gagataacgc tgatcggtga catgcagcgt tccctactcg cgtcacttat ccgcgcaagt
      961 cattcgatgc tatgcgttcg taacgccgcc cggagaccgg gcggcgcgat ccgggcaggt
     1021 gcttgaccgt cgagatgaga acgcttaact tgggacatat gttcgagaga tgcgccgtgg
     1081 tgttcgtgtt cctgctcgcc tcgacggggc tcttgttacc gctgctgcac gagaagggcg
     1141 gtgcggccgc ccagagcgcc gcccaggggg acccggtgac gcaggtcgtc tggttcgggg
     1201 tctacgcggt catgtttttg atgttcctcg tgcagtggcg gcgctttctc ggtatcgctc
     1261 tcaaagacag gctcttgctg ctcctgacgg tcgtcgccct cgcctccgtg ctctgggccg
     1321 tctcgcccga ggtgaccgag cgccgcagcg tggccctgat aggatcgacg ctgttcggca
     1381 tctacctcgc gtcgcgcttc agcttgagcg agatgttgag gctcctggcc tgggccatgg
     1441 gtatagcggt agtcctgagc ctgttgttcg tcctggcgtt gccatcttac ggaatctcgg
     1501 gcgcctccct cacccaggga gactggcagg ggatcttcgg acaaaagaac accctgggca
     1561 agaacatggc cctgagcata accgtgttcc tgatccttgc gatgagcagc cgcagccacc
     1621 ggtggatcgc gctgtctggc atcggcttat catccgtcct tctcctgttt tcgcactcca
     1681 caacttccct ggtggtcgcc gtgagcatca tctgcctcgt gcctttcttt agaggtctac
     1741 gatggaaacg caccctggcg gtatcgctgg tgatcctgac gatattgtta agtggaagct
     1801 ttgccatatg gttcgtgagt aacccgcaga tcgtgctggg agccctcgga aaagatacga
     1861 ccctcaccgg acgcaccgtg ctgtggtccg cggtgctgaa ggcgattcac gaacgtccgt
     1921 ggctgggtta tggttacagc agcttctggc tgggctggga gtacccttcg gccgacgtat
     1981 ggctggcgac ccgcggcaac tactacgact cccataacgg tattctagac ctgtggcttc
     2041 agttaggctt tctggggata tgtatattcg cgctgaactt cctgcgcgcc ttactccggg
     2101 cggtcgcctg ggtacgcgcg actgtaacgg cggaaggaat ctggcccctt gccttcctgt
     2161 cgttcatgct gctgtccaac ctcacggagg gcacgatatt acagcagaac aacctctcct
     2221 ggatcctgta cgtggcgacg gtattctcga tcgctactcc cccgcgaaac ccggccggca
     2281 gaagtaataa tcactccggg gtcggagagt atccggagcc gcttgagcgg cggcgcgctc
     2341 ttatccggta gcggccctcg ccactccgag cagcgcagca agagccgctg atcgtatctt
     2401 gaagacctga tctatggatc tttctacggc agatatccgt ggcgcgtcgt atccctgacc
     2461 cggcatccaa gatatgccac ctgggggatg tgccgggcgc tcctcgacaa atacactgcc
     2521 accacatagc taccctgtcc ctgaaggtct tctcgcttac cggttgggag tagcgaaggt
     2581 caacaatccg aagcgaagag cgtatcagcg ggaggagcca cgagtgaacg gtcgtagaga
     2641 gaaggcccgg taaagagtat gcgcgtgtcc gtgtgcgtaa taacgtaccg ccgggtcgga
     2701 gggctgcgac gcttgctgga gagcctcgaa gggttgatct tcaacaaggc agagcttccc
     2761 gagatggaga tcgtcgtggt ggacaacgat cctgccggat ccgcgcgtgc ggtctgcgaa
     2821 gagttgggat cggattcgag gtggttactg aggtatcaca tcgaaccgcg tcggggcatc
     2881 tctcacgcgc gcaacgcggc ggtggcgttc gcggaagccc cggactttat agcgttcata
     2941 gacgatgacg aggttcccaa accgtactgg ctagacgagc tcctggacgt acaaaaagcg
     3001 tacgccgccg atgtggtcag cggcccggtc ctgacttact tctgcgatcc ggtccccaac
     3061 tgggtcgtca gggggaggtt cttcgagcag gctttcgcca acccgcgcta cgttacgggg
     3121 caccctatcc agctcactgc taccggcaac gtccttatca gggcggagat attcggggat
     3181 gcctcgtttg acgagcgtct gggactaacc ggcggagagg acataaactt cttcgggcgg
     3241 gtgcatcagg cgggttacaa gatggtgtac gcgggagacg ctctcgttta cgagtgggta
     3301 cccggaagcc gcaccaacgt acgctggatc ctgaagagag cctaccggct ggggaatgcc
     3361 ctgagcctca gcgaggcatg cctggacccc acaccggcgg tgcggctcgt ccgcgtcgta
     3421 aagggcggcg ggcggatcgc ccaggggttg ctggtccttc ccctgtttgt gcccctttct
     3481 ctggcccttg ggcggcacct gtccgtaaag cctctgctgc atatttgtcg cggtacgggc
     3541 atgctcgcgg gtctggccgg aatacgcttc gagccgtacc gatgaatctc acgataccag
     3601 gacggaggcg gcggaaggac tcgcaaggaa ggccgcgccg cacagcggcc gaagcgtccg
     3661 gtccaggcag tccattaatg gacaggcctc cttcatcgct gctctttatc catgcctcgg
     3721 cggacctcta cggctccgac attacgctac ttcagatcgt gtccggactg gaccgggaca
     3781 ggttccgcgc cgtcgtggtc gttccttacg ccggacccct agtgccgcgt ctacaggagg
     3841 cgggcgcaga ggtcctagtt tatcccggtc tcccggtcgt ccggcgccag tgcctgcgtc
     3901 tgacgggtct gctgcgtctt acgctctcct cgctcaagac ggccctctgg ctggccgggg
     3961 tcatgcgccg gcgcaacgtg gccctggtgc ataacagcac gctcgccgtg gcgctcgcgg
     4021 gtttggccgc cgggctggcc cgccggccgc agctctggca cgtacaggag atagtcacgc
     4081 gcccgcgcgt cgtcgcctcg ttgctcgcca cgatcagctc ggtggtatcc acggtggtgg
     4141 tcgccaactc gcaggctacc gccggtcatt atcatcgaac ccgctttacc gcatcggcgc
     4201 cggtcagggt gatcccgaac ggcgtcgatg agtctcgcct gcgcgaccgg gccgggacgg
     4261 catcgcgttc gctcgtcgag gcgagcccgg aggacacggt cttcaccctt gtcgggcgca
     4321 taaatcacct gaagggccac gccgtcttcc tcgacgccgc ggaacgcatc gcggccgagt
     4381 cagagaacgt acgcttcctg atcgtgggcg acagcttcgc cgggcaggag gagctcaccg
     4441 aagctgtgga cgagcgcatc gagtcctcgg acctcctccg tgggcgggcg atccggcttc
     4501 ctcacaccgc tgagataggt aacgtctacg aggcgtcgga cgtggtagtc gtgccctcga
     4561 ccgagcccga gtctttcggt ctcgttgccg cggaggccat ggcggcgggg ttgccggtca
     4621 tcgcctccag gatcggcgcc ctcccggaag tggtgaatga cggatctacc ggcatcctgg
     4681 tagatccggg cgacgcgacc tcgctgctgg cggcgatgag gaagctgcac ctttcgccgt
     4741 ccggccgggt caggatgggc cgcctgggga gggagcgctt cgagcgttgt ttccgggtcg
     4801 agacgtacgt cgagggattc aatcgggtgt acgaagaact cttgagaaag gaacgggatt
     4861 atgtcttctc gaacgcgggc cggggataga accatagcct tctg
//
